LOCUS       DAOPNH010000001        56960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_459_length_56960_cov_6.878447, whole genome shotgun sequence.
ACCESSION   DAOPNH010000001 DAOPNH010000000
VERSION     DAOPNH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_459_length_56960_cov_6.878447"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PKZ74_00005"
     CDS             <1..1241
                     /locus_tag="PKZ74_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91156.1"
                     /translation="LEQVFDEEHLVCQGVKLSELVHGQGLPEDNYTICLQAFDYLTGE
                     TVSSESPLGCSNPFTVSDLEPPVILFPVSGQDIFAGDFQNINFVWTWPPGAPNSTRFA
                     LKIIEVLPADRNINDAFRSATQPVFFEKIIASTNYLYGPSDPRLVPGKTYAFSITASD
                     PLQKSFFRNNGESEIARFNFLPEEKPIPASPIEWDVVSSRIIYYNVTSDTLSEDGAPV
                     SGQYAVKLDFRGNGDIIFQTDEKGETQTIACKMTPSGNLTWDNDRGMIPVPVLVSLSA
                     LKDPTSLIVTGDTWSKISVTDGEMNRNIDELILNFKGDFEALTVLQTGEEELICIHTE
                     VYNRIEPTDYSKDFYKNPNVTDGPFNWEKYLSGTVYFNIHLKCLERAEMILLPVFKDQ
                     APKSFKMERRIQNENKPDEK"
     gene            1231..3477
                     /locus_tag="PKZ74_00010"
     CDS             1231..3477
                     /locus_tag="PKZ74_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91157.1"
                     /translation="MKNSRGHFRAGLKRTAIVLLIIAITVGWCELTHNTITSEAINLI
                     LLKNSPAYKEIELFGERMIINQGRRVNKMEDDFDRAYNHYGVNRGFFCKEYLLTPWVV
                     NLYKKHHTPPPASFDMTDFNSAYEWAYSGSVQKNPGDFYLGNEPWNWEGAVRSYDYSM
                     ISKEAAYERLGHVIHMLQDICQPDHFRRIDHPGSSFSTEELDELMKNIRKIRIIPDIV
                     WRAAGVKIPEPVPGAVGFEKLADLYEDARTHELSGLSIRDWKNVGLADGKLFNYFDSA
                     GQGSEYFRSRFGQPALDTALGLHALPVPVNYNIRAASLNTRWIPPLSVKVKIDHVPII
                     PFIVWDMPSNYPNINGYIKLSGNCTKISVEFSAGLIQLFHDIVRHPPYASKINIYQKN
                     KDGQVLTGLSYEAMWEETDDDRNRKFLKTNAEPGLFQACRQTYIEVTFGPSLSVGELK
                     QATQINPESVRVKVGVRNKSEITVDMVLKEGSDNDGSSPPVFTGSFVPPLSWAGSGDG
                     EKFQGYAKIIAEDIFNHYDYGGRAGANSGKILDALPETKARVTDFKPPYPWADYTPGW
                     DETHNDIGSFVLPPPNIEKCRIYTIGGKEFQQYIGTWDDKGCPFPEFIALDRFGGMLL
                     YDRLAAMEVEIYPEVLKIDQASVSVEIGVNGSLVVYTLNNGIRDFQVQDNGFSLKFQP
                     DLSWKSVEDAVKYGYVRIFLNDNSGHRIDANPATVPVIRSRTDWDYYEEGPDLNHQGH
                     VFIEEKPE"
     gene            complement(3516..3806)
                     /locus_tag="PKZ74_00015"
     CDS             complement(3516..3806)
                     /locus_tag="PKZ74_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91158.1"
                     /translation="MEDQTQFQNQNQNPYQNYNAMQNQQPIPVGDWFVTILITAIPLV
                     NIIMLFVWGFSSSTHPSKANWAKASLIWLAIVIVLYIIIFATIGAALFSAYR"
     gene            4056..5912
                     /locus_tag="PKZ74_00020"
     CDS             4056..5912
                     /locus_tag="PKZ74_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91159.1"
                     /translation="MKFRRYFAVCLLIVTASPLFAQPGGYYPPPSVVVFQNDTLTIFP
                     PDSLPGEPVELLSYNILVDDEFYDNLPVLVPAEPAVFIFDITTLTPGNRSFCAKAVYN
                     EWISESACDTAQIFYGMELPVLEDWSSGSFETLQWTTDSENWIIENDEGNPAPSAAFL
                     GNPAVTGYEIALESFPMTAVGMTNGRIWLDFEIKLDANQSTGTEKLFLQVWNWTNETW
                     NAISEYSNFDGSFDWISERILITAKNKVFKVRFLATGENSSAINRWALDNIHIYRTCD
                     GLWDVWLEENPDYNTLEWDGYGGWGWFDGWFNWDDGVFSGNSIGLGEPAEFEFASRWE
                     PAQLIIFPGAVITEVAFVPAEVNADYRIRIWNGSAPDSLLIDQPVQYPVIGEWNYVTL
                     VDTVTIDVTKDLWVGYYVNAQTGYPGGVDDGPAIDGFGNMIYYQDQWQTLKDISDDLD
                     FNWNIACHVIPGLPFPNDKWLYKIYRNTDGGDYLFLDTTFSWNYEDHDIILEEKYCYK
                     VSAVWTENNDTCESDDNPEVCETLCLGVDSPQQDNKVKIYPNPASNWLWIESEDLIRE
                     IKLFNSLGETLMNLEIGNLKFAVYCSSLPAGIYVIEVKTQTTTFRSKFLKID"
     gene            complement(5889..6635)
                     /locus_tag="PKZ74_00025"
     CDS             complement(5889..6635)
                     /locus_tag="PKZ74_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPM91160.1"
                     /translation="MNKLKKIWAEEYPIYWYDTDCNGQLKMSAVANYLQESAWRHANH
                     LGFGYEDARKRNEFWVVISLMIRMIGQPVWGQKIIVETWPKGVDKLFAYRDFKIMDHN
                     GNVIGAATSAWMILDQETRKPKSVDLVKPVLHLATHQDVLDCNPPLLRPVKEIATTEP
                     RKVRFSEVDQNGHVNNIRYLDWSVDALPEEWHKEHRIRTLTINYLSEVMAGEMMMIST
                     GKAEGNSVVVQGTREDGKVAFRTLIDFQEF"
     gene            complement(6869..7429)
                     /locus_tag="PKZ74_00030"
     CDS             complement(6869..7429)
                     /locus_tag="PKZ74_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM91161.1"
                     /translation="MKKSLKILVIAAIAITASFTVQAQKYMTKNGNIKFYSETPIETI
                     EATNNQVNAALDSQTGDLVFKVLIKSFQFEKALMQEHFNENYMESDKFPNSTFVGKVT
                     NLTAVDFSKEGSYEATIEGDLTIHGVTKKISEKGTFTVKAGDKIHGISKFNVKPADYS
                     IKIPGAVVKNIAETIEVTVDIELSKM"
     gene            complement(7468..7908)
                     /locus_tag="PKZ74_00035"
     CDS             complement(7468..7908)
                     /locus_tag="PKZ74_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91162.1"
                     /translation="MKKRTIITIILSLFVIGVISAGLVYKFVINKPHENYEKAKPAFI
                     ISATDLYNSFQSDRTTAEGKFNGKVVLLSGKLDKIDLAESLVTGVFVFNQGMFGDEGI
                     RCVMLGNHAQALKAIGPGTEVKIKGYLTGYNDTDVVLEQCSIIR"
     gene            complement(8016..8444)
                     /locus_tag="PKZ74_00040"
     CDS             complement(8016..8444)
                     /locus_tag="PKZ74_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91163.1"
                     /translation="MKTTAKLFILALTISLASCSKSNDDNPSNTNNTGQVTGNWKVTY
                     YFDSGKDETSDYNGYTFNFNTGNILEAVTGSATYTGTWTLGEPGDDDNSSNKFVITIT
                     GNKAMDDLTDDWKILKMTDTEIWLQDDNLTSAEELRFGKL"
     gene            complement(8594..9352)
                     /locus_tag="PKZ74_00045"
     CDS             complement(8594..9352)
                     /locus_tag="PKZ74_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91164.1"
                     /translation="MKLLIIEDEPASAQRLKKMVEEMDPEVKVLGILDSITSTMDWFG
                     HHPEPDLILSDIHLADGLSFEIFRQINISCPVIFTTAYDQYAIQAFKLNSIDYLLKPV
                     RKEELAEAINKFKKVKPAEKQVDLSQLMALIGKQEKEYLRRVVIRLGQQIKIVEIKDV
                     AYFYIDEKIVFGVTFGKDRYPMDLSLDQLEKQLDPGRFFRINRAFIISLDSIETMITY
                     SKARIKIKLKPPCELESITSTERSAEFREWLRGG"
     gene            complement(9461..12337)
                     /locus_tag="PKZ74_00050"
     CDS             complement(9461..12337)
                     /locus_tag="PKZ74_00050"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM91165.1"
                     /translation="MFWLTAFIFLIAHSGFSQLPGFEKIRITVPGEGPVFKKAVMDAD
                     GYIWLGSGQGLFRFDGIQFKSFTPLPDTADFHVTALHLDQDHDLWIGCRDGKIYRLDQ
                     GRINLFNPEEGTSGVAVTDILVAQDRSIWWSTAGEGIYFYSGTRVFNINRDDGLPDDY
                     VHDLESVSDGYVVAGTDGGLVSCIYRNGEKIVNPLAASGELPDIMVTVVKEDSDGNLW
                     LGFQDGGIGFLTNDGRQFIQPAADHAWEEGPVSAVTVSGASAWAGTSSGELLEISLNE
                     NNRGMINLLPADLQTGKIYDLLEDREGNIWILSAEGLFRSTGNRIRYLDDVGGIPLAN
                     IHAIHADEFRPGLLWFSDDRGLFSLAVESGDVKSYPGPFKGPGLKITCLNQDRFGYIW
                     AGTFNYGAIRLNPANGTFDRITERDNLVNNNVLSISRHHDTLWMATLGGASEIILDPN
                     TSGRSYQARSYNYENGLVNNFIYYVYEDDKNRIWLATDGDGINVRTQDGWSSYNENNG
                     LTDDVIYSLTGDKYGNIWAGSASGGLFKFDGKNFIHYGLDEGLSSISFTGIMAADDEL
                     LAVHENGLDILHIPTGNIAHYGGDKGFEGIQPDLNTISRDREGNIWLGTRKGIIRYRP
                     GSAVSYMPVTVLEEMSVYLEPRELKAGQVFGYADNHISFRYAGLWFSNPERVNYQVML
                     EGYDLGWNDTYNRQVTYSSLPPGEFTFKVRSSLDRSFVNAGEVSYSFRIRQPVWIQPW
                     FIILLVIILAALIFLYIKMRENRLRKREQEKKEKIEFEFQVLKNQVNPHFLFNSFSTL
                     ISLIEDNPEQAVQYTEKLSDFFRTILQYKDQELITLDEELTLIQSYFFLLKKRYGDNL
                     YLDIQLGDELKKAFIPPMTLQILIENAVKHNVISMSKPLFIKVYEDDGKIVVENNLQP
                     KLTPEVSTGIGLENIRKRYKLITDKEVIIEISELLFRIFLPLIK"
     gene            complement(12369..12920)
                     /locus_tag="PKZ74_00055"
     CDS             complement(12369..12920)
                     /locus_tag="PKZ74_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM91166.1"
                     /translation="MIKHILFIIIVLLANNSFCQPVYKTKNGTASFVSNAPLEIIRAE
                     SAEVQGAIDPGKKTFAFSIDNKSFKGFNSPLQQEHFYENYLEVQSFPASTFKGKIIED
                     IDLNSSLEQTVRAKGMLNIHGVEQERIIKGTIRVVDGKISLKADFSVMLEDHQIRIPR
                     VVNQKIAESIDVSVTAELVKSND"
     gene            13049..14095
                     /locus_tag="PKZ74_00060"
     CDS             13049..14095
                     /locus_tag="PKZ74_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM91167.1"
                     /translation="MKILFIILIVILIIILGSLLLGWLFSAPVYHGPVSDHFDGKRFI
                     NPGGKKAGGLKDVIKWSRERQRGEWKEITEVNYGPAPEERVEGDSIVVTFVNHSTFLI
                     QTQGLNILTDPVWSERVSPVTFAGPKRMRQPGIRFEELPPIDIILLTHNHYDHLDIST
                     LKKLTSRFKPVIYTPLGVGLYLKKKGIDNFREMDWWDELTTPNGLKLVCTPAQHFSGR
                     GMFDRDRTLWSGFALRTENGSIYYSGDTGYGEFFNEIAERLSPIRLSFLPIGAYKPQW
                     FMSPIHTSPADAVNIHNILKSPQSIGMHFGTFPLADDGISDPAKELKEAKSREGILDE
                     RFLILEEGRAYSFH"
     gene            15227..16312
                     /gene="carA"
                     /locus_tag="PKZ74_00065"
     CDS             15227..16312
                     /gene="carA"
                     /locus_tag="PKZ74_00065"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPM91168.1"
                     /translation="MNKTVPATLILDDGTEFRGYSFGAEQSSSGEVVFSTAMNGYPES
                     LTDPSYKGQILVATYPLIGNYGVPEMRMKEGMTEFFESDKVQISGLVISDYSFSYSHW
                     NAVKSLSDWLKEHRVPGIAGVDTRKLTRIIREKGAMLGKIVVQDDVHFYDPNKDNLVN
                     QVSAREKITYGNGRRRILLVDCGTKNNIIRCLLKRDATVHRVPWDHPADPAEYDGILV
                     SNGPGDPKMCKATIESVKNLLDGDLPVFGICLGSQIMALAAGADTYKLKYGHRSHNQP
                     VIMKESHRCFITSQNHGYAVDTETLPEGWKPWFENLNDGTCEGIIHSSKPFFAVQFHP
                     EASAGPVDTEFLFDHFMEKVREYEARH"
     gene            16296..19652
                     /gene="carB"
                     /locus_tag="PKZ74_00070"
     CDS             16296..19652
                     /gene="carB"
                     /locus_tag="PKZ74_00070"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPM91169.1"
                     /translation="MKHAIKKVLLLGSGALKIGEAGEFDYSGTQALKALREEGIATVL
                     INPNIATVQTSEGMADKIYFLPVTPFFVEKVIEKENPDGILLSFGGQTALNCGVALHQ
                     SGILEKHGVRVLGTPVSAILDTEDRELFTRKLDQIHVKTPRSIAVTGVDEAVQAAATL
                     GYPVIVRSAFALGGLGSGFCGSEDEMRALTQKSFSYSSQVLVEESLKGWKEVEYEVVR
                     DGFDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNREYHKLRELSIRIVRHIGITGE
                     CNVQYALDPNSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKIALGYGLFELKNSV
                     TQDTSAFFEPALDYIVVKIPRWDLGKFHGVSREIGSSMKSVGEVMAIGRTFEEAIQKG
                     LRMIGQGMHGFVGNKDIEDIIIDKELAVPTDMRIFVIAMALMKGYSVERIFELTKIDR
                     WFLEKLKNIWDIRNEVITYNTLEELPLALLLRSKQLGFSDFQIARFIFKSDRDHIADD
                     LLKVRNYRKAKGILPYVKQIDTMAAEYPARTNYLYLTYNGNEHDIGFSAKNVTTPTTK
                     AVGSEDGLNLEPTALVVGRKEQREPTASVVGLQKEETPETVVVLGSGAYRIGSSVEFD
                     WCGVNALECVNKEGYRSVMINYNPETVSTDYDLCDRLYFEELTFERVMDILDLENPLG
                     VIVSTGGQIPNNLAIRLWEQEVAILGTSPQSIDKAENRHKFSAILDELGIDQPRWIES
                     SSVSEVYRFTDEVGFPVIVRPSYVLSGAAMNIVSNRSELEHFLEMAAEVSKQYPVVVS
                     RFIEDAKEIEMDAVAANGEIICYAISEHVEFAGVHSGDATMLFPPQKIYVETVRRIKR
                     ISRQIAQHLKISGPFNIQFLAKDNDVMVIECNLRASRSFPFISKVLKTNFIETATKIM
                     LGKTVAKPDKSLFDLDYVGVKASQFSFSRLQKADPVLGVEMVSTGEVGCIGDNYYEAI
                     LKSMLSVGYTIPEKAVLYSSGPSRSKQELLTSARMLQEKGLTIYATGGTHDFFIKNGI
                     NSIMLHWPGEDKTPNTMEYLRRKKIDLVINIPKDLSKSELDNDYRIRRSAVDFNIPLI
                     TNARLASAFIYSFCRIGIEEIGIKAWGEYE"
     gene            complement(19717..20178)
                     /locus_tag="PKZ74_00075"
     CDS             complement(19717..20178)
                     /locus_tag="PKZ74_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM91170.1"
                     /translation="MTIVTTTRLMNRKIFLTKPGIYCEKMGLIRKNNMNRIELEKRLI
                     SFASAIITLCKECRFDDYTFHLAKQIVRSASSAALNYGEAQSAESRADFTHKLSLVLK
                     ELRETQVNITIMSNSVICNNPLRIVDLLKECQDLIAIFQKSINTLNNKNNR"
     gene            20316..22100
                     /locus_tag="PKZ74_00080"
     CDS             20316..22100
                     /locus_tag="PKZ74_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM91171.1"
                     /translation="MIGQKYNNNLQYLLIVVAGALLFLPFLGNVALFDWDEVNFAEIA
                     REMIVTGDYLNVQINYQPFWEKPPLFIWMQALSMKLFGINEFAARFPNAICGITTLLV
                     IFAIGKKIHNEKMGLTWVLVYAGSILPFFYFKSGIIDPWFNLFIFLGIYFATISIDRS
                     RVQLFNGSIVNEPDISSYNPTVLQSNSHKFLHSPGLLVFLSGAAIGLAILTKGPVALL
                     IFGLVAIVFPFSFILTNIKKSTTSQNQISKFPNFQIITKSLSLFAIGLLLTGGSWFLL
                     MIANGHLDVVIDFIVYQVRLFSTKDAGHGGFPGYHVVILMLGVFPASAFFFGGLKAVP
                     EENFLQKNWRRVMIILLATVVVLFEIVQTKIVHYSSLAYFPLTYLAALALYRASLSER
                     AFSRSSRIILWTLAILWMTVIAGFTLVGLNKVWLMNSGLIKDPFAVANLAADIRWTGF
                     ETLVALVLIPGIAGFFILKKPFHKMIALFSTSLVFTFLTMTIFTGKIEGYVQRAPIEF
                     YEKHSSEECYINTLGFKSYAHLFYGKKMPPVNPISYDKEWLLNGKIDKNAYFVYKITK
                     KEEYSRLYPQLTVLYEKNGFIFAARYPE"
     gene            22115..22852
                     /locus_tag="PKZ74_00085"
     CDS             22115..22852
                     /locus_tag="PKZ74_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91172.1"
                     /translation="MIYGKKITVVLPAYNAEKTLEQTYREIPFDIVDEVILVDDKSRD
                     NTVEVAKRLGIHHIIEHQENKGYGGNQKSCYDKALEIGSDIVVMLHPDYQYTPKLIHS
                     MCYVIANDVYPVVLGSRILGKGALRGGMPLYKYISNRFLTLAQNMLMKQKLSEYHTGY
                     RAFSKEVLQNIKYQHNSDDFIFDNQMLAQIFFAGYEIAEVTCPTKYFDDASSINLKRS
                     MKYGLGVLGVSFRYFLHKHRIARYRIF"
     gene            complement(22934..25228)
                     /locus_tag="PKZ74_00090"
     CDS             complement(22934..25228)
                     /locus_tag="PKZ74_00090"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPM91173.1"
                     /translation="MKKIYLLFMICLMGVAVFAQPVGRQKVIVEIGTGTWCTYCPGAA
                     MGADDLVLYGCQVGNIEYHNGDPYANTASDYRNSYYNVSGYPTAHFDGVLEYVGGSHT
                     ESMYPNYLPLYQQRMAIPSDFLIDIYGEHTGNTYNIQLVISQTNGTWGNLTVHLVLTE
                     SEIVYSWQGQDHLNFVERLMAPTHMGTVVDFTSQDEVTLDLSFTMDASWVASNCELVA
                     FVQNEATKEVLQGNMVAIPDLEPMQATAGFTCEDVTPCITTAVEFEDQSMGEIISWNW
                     TFEAGNPATSAAQNPVVTYNTQGQYDVQLIVYDGTVYDTLLNPNYILVITPPVQPQTP
                     NGPTTVCQENDGIQYSTPLVQWATTYVWDVEPDAAGTISGPDPVATFTLAPGYLGTYT
                     IKVRADNDCGNGTWSQGLNCAAYMTPSEYTLSDGSGYCEGSDGIELTLDGSQNGVDYE
                     LYLDGDPTGQIMAGTGSAITFGNQTEQGIYTCVGYTDYCDKNMIGNAYIFIITPPDEG
                     ETPSGETQVCSSDSGTEYISDDIDGATSYFWNLSPTEAGSISGNDETATVDWNAEFSG
                     DAYISVAGVNSCFTGDFSDALTVVVNAAPQPVISGPQLVCDYEVGDIYSTELADGNDY
                     TWEINGGTITEGNGTNEITVTWGAAGDGWVKVTETNGVCSFTTLEYEVVIDECIGVGE
                     NGMASFMIYPNPVKEEMMISFTGKTTNCRMVILNQLGQVMIDQMTGNNKQISVNTSML
                     SKGLYSIVVYTDNGLIEKKFVKTE"
     gene            complement(25473..25619)
                     /locus_tag="PKZ74_00095"
     CDS             complement(25473..25619)
                     /locus_tag="PKZ74_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91174.1"
                     /translation="MVAAFRKQPDIAIGNVIGSNIFNILSILGIAAMVEPIEAPGSIL
                     PIPW"
     gene            complement(25814..26767)
                     /locus_tag="PKZ74_00100"
     CDS             complement(25814..26767)
                     /locus_tag="PKZ74_00100"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPM91175.1"
                     /translation="MRVVEAIAKSRSPLFTFEILPPVKGSDINSIYRAIDPLMEFEPA
                     FIEVTYHREEILYKEFPDGSIEKRVLRKRPGTVGISAAIKYKYKIEVVPHIICGGFNR
                     EETENGLIDLHFLEIYNLLAIRGDNLPGEKFFKPEKGGNQFAVDLIRQIMDLNKGKYL
                     DNELVNNAPTRFSVGVAGYPEKHAEAPNMRTDIRHLKEKIDAGGEYIVTQMFFENRFY
                     FEFVKQCREAGITVPIIPGLKPVSTAGQLNTLPKTFNISIPEDLQKEVEKCRDSKQVR
                     QVGIEWCTMQSKELMQAGVPALHYFTMGNSDNIREIVKHIF"
     gene            complement(27005..28663)
                     /locus_tag="PKZ74_00105"
     CDS             complement(27005..28663)
                     /locus_tag="PKZ74_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPM91176.1"
                     /translation="MLLLCCLQDFLGRHHHPQVDHFIIIAPQYYTHDVFADIVHIALD
                     SGKKHSGPPPPLGPLPQLGEGTKGRGRARVLLATVKGDVHDIGKNIVGVVLGCNNYEV
                     IDLGVMVPAEKILQTAQEQHVDVIGLSGLITPSLEEMAHVASEMQRLNFDIPLLIGGA
                     TTSEIHTAVKIAPRYDHPVIHVRDASRCVGVLSSLLSPENKKTYTQEIAEKYRNLKIK
                     HENRKNEDVLIPLHEARKNRLSVDWKNIEITAPAFTGITVFGDYPLAELRDYIDWTFF
                     FHAWKIPGKYPEIFDDPVKGEEAGKLFADAQKMIEEILDKGMLKASGVIGLFPAVSRG
                     DDVVLFKDEKMTESLSELHFLRNQEPKRDGVPNLSFADFIAPESYGKTDYIGLFAVTA
                     GIGIEKWVKHYEDALDDYSSFMLKILADRFAEAFAERLHERVRKEFWGYAAGENIPVS
                     DMLKEEYRGIRPAPGYPGCPEHSEKRAIFDLLKAEENAGINLTENFAMYPAASVSGYY
                     FSHDFSRYFNLGKITDDQVRDYAERKNISSDLARKYLRQNLMDE"
     gene            28568..29956
                     /locus_tag="PKZ74_00110"
     CDS             28568..29956
                     /locus_tag="PKZ74_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPM91177.1"
                     /translation="MGVVLGCNNYEVIDLGVMVPAEKILQTAQEQHVDVIGLSGLITP
                     SLEEMAHVASEMQRLNFDIPLLIGGATTSEIHTAVKIAPRYDHPVIHVRDASRCVGVL
                     SSLLSPENKKTYTQEIAEKYRNLKIKHENRKNEDVLIPLHEARKNRLSVDWKNIEITA
                     PAFTGITVFGDYPLAELRDYIDWTFFFHAWKIPGKYPEIFDDPVKGEEAGKLFADAQK
                     MIEEILDKGMLKASGVIGLFPAVSRGDDVVLFKDEKMTESLSELHFLRNQEPKRDGVP
                     NLSFADFIAPESYGKTDYIGLFAVTAGIGIEKWVKHYEDALDDYSSFMLKILADRFAE
                     AFAERLHERVRKEFWGYAAGENIPVSDMLKEEYRGIRPAPGYPGCPEHSEKRAIFDLL
                     KAEENAGINLTENFAMYPAASVSGYYFSHDFSRYFNLGKITDDQVRDYAERKNISSDL
                     ARKYLRQNLMDE"
     gene            30194..31147
                     /locus_tag="PKZ74_00115"
     CDS             30194..31147
                     /locus_tag="PKZ74_00115"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPM91178.1"
                     /translation="MRVVEAIAKSRSPLFTFEILPPVKGSDINSIYRAIDPLMEFEPA
                     FIEVTYHREEILYKEFPDGSIEKRVLRKRPGTVGISAAIKYKYKIEVVPHIICGGFNR
                     EETENGLIDLHFLEIYNLLAIRGDNLPGEKFFKPEKGGNQFAVDLIRQIMDLNKGKYL
                     DNELVNNAPTRFSVGVAGYPEKHAEAPNMRTDIRHLKEKIDAGGEYIVTQMFFENRFY
                     FEFVKQCREAGITVPIIPGLKPVSTAGQLNTLPKTFNISIPEDLQKEVEKCRDSKQVR
                     QVGIEWCTMQSKELMQAGVPALHYFTMGNSDNIREIVKHIF"
     gene            31342..31488
                     /locus_tag="PKZ74_00120"
     CDS             31342..31488
                     /locus_tag="PKZ74_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91179.1"
                     /translation="MVAAFRKQPDIAIGNVIGSNIFNILSILGIAAMVEPIEAPGSIL
                     PIPW"
     gene            31733..34027
                     /locus_tag="PKZ74_00125"
     CDS             31733..34027
                     /locus_tag="PKZ74_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPM91180.1"
                     /translation="MKKIYLLFMICLMGVAVFAQPVGRQKVIVEIGTGTWCTYCPGAA
                     MGADDLVLYGCQVGNIEYHNGDPYANTASDYRNSYYNVSGYPTAHFDGVLEYVGGSHT
                     ESMYPNYLPLYQQRMAIPSDFLIDIYGEHTGNTYNIQLVISQTNGTWGNLTVHLVLTE
                     SEIVYSWQGQDHLNFVERLMAPTHMGTVVDFTSQDEVTLDLSFTMDASWVASNCELVA
                     FVQNEATKEVLQGNMVAIPDLEPMQATAGFTCEDVTPCITTAVEFEDQSMGEIISWNW
                     TFEAGNPATSAAQNPVVTYNTQGQYDVQLIVYDGTVYDTLLNPNYILVITPPVQPQTP
                     NGPTTVCQENDGIQYSTPLVQWATTYVWDVEPDAAGTISGPDPVATFTLAPGYLGTYT
                     IKVRADNDCGNGTWSQGLNCAAYMTPSEYTLSDGSGYCEGSDGIELTLDGSQNGVDYE
                     LYLDGDPTGQIMAGTGSAITFGNQTEQGIYTCVGYTDYCDKNMIGNAYIFIITPPDEG
                     ETPSGETQVCSSDSGTEYISDDIDGATSYFWNLSPTEAGSISGNDETATVDWNAEFSG
                     DAYISVAGVNSCFTGDFSDALTVVVNAAPQPVISGPQLVCDYEVGDIYSTELADGNDY
                     TWEINGGTITEGNGTNEITVTWGAAGDGWVKVTETNGVCSFTTLEYEVVIDECIGVGE
                     NGMASFMIYPNPVKEEMMISFTGKTTNCRMVILNQLGQVMIDQMTGNNKQISVNTSML
                     SKGLYSIVVYTDNGLIEKKFVKTE"
     gene            complement(34109..34846)
                     /locus_tag="PKZ74_00130"
     CDS             complement(34109..34846)
                     /locus_tag="PKZ74_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91181.1"
                     /translation="MIYGKKITVVLPAYNAEKTLEQTYREIPFDIVDEVILVDDKSRD
                     NTVEVAKRLGIHHIIEHQENKGYGGNQKSCYDKALEIGSDIVVMLHPDYQYTPKLIHS
                     MCYVIANDVYPVVLGSRILGKGALRGGMPLYKYISNRFLTLAQNMLMKQKLSEYHTGY
                     RAFSKEVLQNIKYQHNSDDFIFDNQMLAQIFFAGYEIAEVTCPTKYFDDASSINLKRS
                     MKYGLGVLGVSFRYFLHKHRIARYRIF"
     gene            complement(34861..36645)
                     /locus_tag="PKZ74_00135"
     CDS             complement(34861..36645)
                     /locus_tag="PKZ74_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM91182.1"
                     /translation="MIGQKYNNNLQYLLIVVAGALLFLPFLGNVALFDWDEVNFAEIA
                     REMIVTGDYLNVQINYQPFWEKPPLFIWMQALSMKLFGINEFAARFPNAICGITTLLV
                     IFAIGKKIHNEKMGLTWVLVYAGSILPFFYFKSGIIDPWFNLFIFLGIYFATISIDRS
                     RVQLFNGSIVNEPDISSYNPTVLQSNSHKFLHSPGLLVFLSGAAIGLAILTKGPVALL
                     IFGLVAIVFPFSFILTNIKKSTTSQNQISKFPNFQIITKSLSLFAIGLLLTGGSWFLL
                     MIANGHLDVVIDFIVYQVRLFSTKDAGHGGFPGYHVVILMLGVFPASAFFFGGLKAVP
                     EENFLQKNWRRVMIILLATVVVLFEIVQTKIVHYSSLAYFPLTYLAALALYRASLSER
                     AFSRSSRIILWTLAILWMTVIAGFTLVGLNKVWLMNSGLIKDPFAVANLAADIRWTGF
                     ETLVALVLIPGIAGFFILKKPFHKMIALFSTSLVFTFLTMTIFTGKIEGYVQRAPIEF
                     YEKHSSEECYINTLGFKSYAHLFYGKKMPPVNPISYDKEWLLNGKIDKNAYFVYKITK
                     KEEYSRLYPQLTVLYEKNGFIFAARYPE"
     gene            36783..37244
                     /locus_tag="PKZ74_00140"
     CDS             36783..37244
                     /locus_tag="PKZ74_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM91183.1"
                     /translation="MTIVTTTRLMNRKIFLTKPGIYCEKMGLIRKNNMNRIELEKRLI
                     SFASAIITLCKECRFDDYTFHLAKQIVRSASSAALNYGEAQSAESRADFTHKLSLVLK
                     ELRETQVNITIMSNSVICNNPLRIVDLLKECQDLIAIFQKSINTLNNKNNR"
     gene            complement(37309..40665)
                     /gene="carB"
                     /locus_tag="PKZ74_00145"
     CDS             complement(37309..40665)
                     /gene="carB"
                     /locus_tag="PKZ74_00145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPM91184.1"
                     /translation="MKHAIKKVLLLGSGALKIGEAGEFDYSGTQALKALREEGIATVL
                     INPNIATVQTSEGMADKIYFLPVTPFFVEKVIEKENPDGILLSFGGQTALNCGVALHQ
                     SGILEKHGVRVLGTPVSAILDTEDRELFTRKLDQIHVKTPRSIAVTGVDEAVQAAATL
                     GYPVIVRSAFALGGLGSGFCGSEDEMRALTQKSFSYSSQVLVEESLKGWKEVEYEVVR
                     DGFDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNREYHKLRELSIRIVRHIGITGE
                     CNVQYALDPNSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKIALGYGLFELKNSV
                     TQDTSAFFEPALDYIVVKIPRWDLGKFHGVSREIGSSMKSVGEVMAIGRTFEEAIQKG
                     LRMIGQGMHGFVGNKDIEDIIIDKELAVPTDMRIFVIAMALMKGYSVERIFELTKIDR
                     WFLEKLKNIWDIRNEVITYNTLEELPLALLLRSKQLGFSDFQIARFIFKSDRDHIADD
                     LLKVRNYRKAKGILPYVKQIDTMAAEYPARTNYLYLTYNGNEHDIGFSAKNVTTPTTK
                     AVGSEDGLNLEPTALVVGRKEQREPTASVVGLQKEETPETVVVLGSGAYRIGSSVEFD
                     WCGVNALECVNKEGYRSVMINYNPETVSTDYDLCDRLYFEELTFERVMDILDLENPLG
                     VIVSTGGQIPNNLAIRLWEQEVAILGTSPQSIDKAENRHKFSAILDELGIDQPRWIES
                     SSVSEVYRFTDEVGFPVIVRPSYVLSGAAMNIVSNRSELEHFLEMAAEVSKQYPVVVS
                     RFIEDAKEIEMDAVAANGEIICYAISEHVEFAGVHSGDATMLFPPQKIYVETVRRIKR
                     ISRQIAQHLKISGPFNIQFLAKDNDVMVIECNLRASRSFPFISKVLKTNFIETATKIM
                     LGKTVAKPDKSLFDLDYVGVKASQFSFSRLQKADPVLGVEMVSTGEVGCIGDNYYEAI
                     LKSMLSVGYTIPEKAVLYSSGPSRSKQELLTSARMLQEKGLTIYATGGTHDFFIKNGI
                     NSIMLHWPGEDKTPNTMEYLRRKKIDLVINIPKDLSKSELDNDYRIRRSAVDFNIPLI
                     TNARLASAFIYSFCRIGIEEIGIKAWGEYE"
     gene            complement(40649..41734)
                     /gene="carA"
                     /locus_tag="PKZ74_00150"
     CDS             complement(40649..41734)
                     /gene="carA"
                     /locus_tag="PKZ74_00150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPM91185.1"
                     /translation="MNKTVPATLILDDGTEFRGYSFGAEQSSSGEVVFSTAMNGYPES
                     LTDPSYKGQILVATYPLIGNYGVPEMRMKEGMTEFFESDKVQISGLVISDYSFSYSHW
                     NAVKSLSDWLKEHRVPGIAGVDTRKLTRIIREKGAMLGKIVVQDDVHFYDPNKDNLVN
                     QVSAREKITYGNGRRRILLVDCGTKNNIIRCLLKRDATVHRVPWDHPADPAEYDGILV
                     SNGPGDPKMCKATIESVKNLLDGDLPVFGICLGSQIMALAAGADTYKLKYGHRSHNQP
                     VIMKESHRCFITSQNHGYAVDTETLPEGWKPWFENLNDGTCEGIIHSSKPFFAVQFHP
                     EASAGPVDTEFLFDHFMEKVREYEARH"
     gene            complement(42866..43912)
                     /locus_tag="PKZ74_00155"
     CDS             complement(42866..43912)
                     /locus_tag="PKZ74_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM91186.1"
                     /translation="MKILFIILIVILIIILGSLLLGWLFSAPVYHGPVSDHFDGKRFI
                     NPGGKKAGGLKDVIKWSRERQRGEWKEITEVNYGPAPEERVEGDSIVVTFVNHSTFLI
                     QTQGLNILTDPVWSERVSPVTFAGPKRMRQPGIRFEELPPIDIILLTHNHYDHLDIST
                     LKKLTSRFKPVIYTPLGVGLYLKKKGIDNFREMDWWDELTTPNGLKLVCTPAQHFSGR
                     GMFDRDRTLWSGFALRTENGSIYYSGDTGYGEFFNEIAERLSPIRLSFLPIGAYKPQW
                     FMSPIHTSPADAVNIHNILKSPQSIGMHFGTFPLADDGISDPAKELKEAKSREGILDE
                     RFLILEEGRAYSFH"
     gene            44041..44592
                     /locus_tag="PKZ74_00160"
     CDS             44041..44592
                     /locus_tag="PKZ74_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM91187.1"
                     /translation="MIKHILFIIIVLLANNSFCQPVYKTKNGTASFVSNAPLEIIRAE
                     SAEVQGAIDPGKKTFAFSIDNKSFKGFNSPLQQEHFYENYLEVQSFPASTFKGKIIED
                     IDLNSSLEQTVRAKGMLNIHGVEQERIIKGTIRVVDGKISLKADFSVMLEDHQIRIPR
                     VVNQKIAESIDVSVTAELVKSND"
     gene            44624..47500
                     /locus_tag="PKZ74_00165"
     CDS             44624..47500
                     /locus_tag="PKZ74_00165"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM91188.1"
                     /translation="MFWLTAFIFLIAHSGFSQLPGFEKIRITVPGEGPVFKKAVMDAD
                     GYIWLGSGQGLFRFDGIQFKSFTPLPDTADFHVTALHLDQDHDLWIGCRDGKIYRLDQ
                     GRINLFNPEEGTSGVAVTDILVAQDRSIWWSTAGEGIYFYSGTRVFNINRDDGLPDDY
                     VHDLESVSDGYVVAGTDGGLVSCIYRNGEKIVNPLAASGELPDIMVTVVKEDSDGNLW
                     LGFQDGGIGFLTNDGRQFIQPAADHAWEEGPVSAVTVSGASAWAGTSSGELLEISLNE
                     NNRGMINLLPADLQTGKIYDLLEDREGNIWILSAEGLFRSTGNRIRYLDDVGGIPLAN
                     IHAIHADEFRPGLLWFSDDRGLFSLAVESGDVKSYPGPFKGPGLKITCLNQDRFGYIW
                     AGTFNYGAIRLNPANGTFDRITERDNLVNNNVLSISRHHDTLWMATLGGASEIILDPN
                     TSGRSYQARSYNYENGLVNNFIYYVYEDDKNRIWLATDGDGINVRTQDGWSSYNENNG
                     LTDDVIYSLTGDKYGNIWAGSASGGLFKFDGKNFIHYGLDEGLSSISFTGIMAADDEL
                     LAVHENGLDILHIPTGNIAHYGGDKGFEGIQPDLNTISRDREGNIWLGTRKGIIRYRP
                     GSAVSYMPVTVLEEMSVYLEPRELKAGQVFGYADNHISFRYAGLWFSNPERVNYQVML
                     EGYDLGWNDTYNRQVTYSSLPPGEFTFKVRSSLDRSFVNAGEVSYSFRIRQPVWIQPW
                     FIILLVIILAALIFLYIKMRENRLRKREQEKKEKIEFEFQVLKNQVNPHFLFNSFSTL
                     ISLIEDNPEQAVQYTEKLSDFFRTILQYKDQELITLDEELTLIQSYFFLLKKRYGDNL
                     YLDIQLGDELKKAFIPPMTLQILIENAVKHNVISMSKPLFIKVYEDDGKIVVENNLQP
                     KLTPEVSTGIGLENIRKRYKLITDKEVIIEISELLFRIFLPLIK"
     gene            47609..48367
                     /locus_tag="PKZ74_00170"
     CDS             47609..48367
                     /locus_tag="PKZ74_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91189.1"
                     /translation="MKLLIIEDEPASAQRLKKMVEEMDPEVKVLGILDSITSTMDWFG
                     HHPEPDLILSDIHLADGLSFEIFRQINISCPVIFTTAYDQYAIQAFKLNSIDYLLKPV
                     RKEELAEAINKFKKVKPAEKQVDLSQLMALIGKQEKEYLRRVVIRLGQQIKIVEIKDV
                     AYFYIDEKIVFGVTFGKDRYPMDLSLDQLEKQLDPGRFFRINRAFIISLDSIETMITY
                     SKARIKIKLKPPCELESITSTERSAEFREWLRGG"
     gene            48517..48945
                     /locus_tag="PKZ74_00175"
     CDS             48517..48945
                     /locus_tag="PKZ74_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91190.1"
                     /translation="MKTTAKLFILALTISLASCSKSNDDNPSNTNNTGQVTGNWKVTY
                     YFDSGKDETSDYNGYTFNFNTGNILEAVTGSATYTGTWTLGEPGDDDNSSNKFVITIT
                     GNKAMDDLTDDWKILKMTDTEIWLQDDNLTSAEELRFGKL"
     gene            49053..49493
                     /locus_tag="PKZ74_00180"
     CDS             49053..49493
                     /locus_tag="PKZ74_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91191.1"
                     /translation="MKKRTIITIILSLFVIGVISAGLVYKFVINKPHENYEKAKPAFI
                     ISATDLYNSFQSDRTTAEGKFNGKVVLLSGKLDKIDLAESLVTGVFVFNQGMFGDEGI
                     RCVMLGNHAQALKAIGPGTEVKIKGYLTGYNDTDVVLEQCSIIR"
     gene            49532..50092
                     /locus_tag="PKZ74_00185"
     CDS             49532..50092
                     /locus_tag="PKZ74_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM91192.1"
                     /translation="MKKSLKILVIAAIAITASFTVQAQKYMTKNGNIKFYSETPIETI
                     EATNNQVNAALDSQTGDLVFKVLIKSFQFEKALMQEHFNENYMESDKFPNSTFVGKVT
                     NLTAVDFSKEGSYEATIEGDLTIHGVTKKISEKGTFTVKAGDKIHGISKFNVKPADYS
                     IKIPGAVVKNIAETIEVTVDIELSKM"
     gene            50326..51072
                     /locus_tag="PKZ74_00190"
     CDS             50326..51072
                     /locus_tag="PKZ74_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPM91193.1"
                     /translation="MNKLKKIWAEEYPIYWYDTDCNGQLKMSAVANYLQESAWRHANH
                     LGFGYEDARKRNEFWVVISLMIRMIGQPVWGQKIIVETWPKGVDKLFAYRDFKIMDHN
                     GNVIGAATSAWMILDQETRKPKSVDLVKPVLHLATHQDVLDCNPPLLRPVKEIATTEP
                     RKVRFSEVDQNGHVNNIRYLDWSVDALPEEWHKEHRIRTLTINYLSEVMAGEMMMIST
                     GKAEGNSVVVQGTREDGKVAFRTLIDFQEF"
     gene            complement(51049..52905)
                     /locus_tag="PKZ74_00195"
     CDS             complement(51049..52905)
                     /locus_tag="PKZ74_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91194.1"
                     /translation="MKFRRYFAVCLLIVTASPLFAQPGGYYPPPSVVVFQNDTLTIFP
                     PDSLPGEPVELLSYNILVDDEFYDNLPVLVPAEPAVFIFDITTLTPGNRSFCAKAVYN
                     EWISESACDTAQIFYGMELPVLEDWSSGSFETLQWTTDSENWIIENDEGNPAPSAAFL
                     GNPAVTGYEIALESFPMTAVGMTNGRIWLDFEIKLDANQSTGTEKLFLQVWNWTNETW
                     NAISEYSNFDGSFDWISERILITAKNKVFKVRFLATGENSSAINRWALDNIHIYRTCD
                     GLWDVWLEENPDYNTLEWDGYGGWGWFDGWFNWDDGVFSGNSIGLGEPAEFEFASRWE
                     PAQLIIFPGAVITEVAFVPAEVNADYRIRIWNGSAPDSLLIDQPVQYPVIGEWNYVTL
                     VDTVTIDVTKDLWVGYYVNAQTGYPGGVDDGPAIDGFGNMIYYQDQWQTLKDISDDLD
                     FNWNIACHVIPGLPFPNDKWLYKIYRNTDGGDYLFLDTTFSWNYEDHDIILEEKYCYK
                     VSAVWTENNDTCESDDNPEVCETLCLGVDSPQQDNKVKIYPNPASNWLWIESEDLIRE
                     IKLFNSLGETLMNLEIGNLKFAVYCSSLPAGIYVIEVKTQTTTFRSKFLKID"
     gene            53155..53445
                     /locus_tag="PKZ74_00200"
     CDS             53155..53445
                     /locus_tag="PKZ74_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91195.1"
                     /translation="MEDQTQFQNQNQNPYQNYNAMQNQQPIPVGDWFVTILITAIPLV
                     NIIMLFVWGFSSSTHPSKANWAKASLIWLAIVIVLYIIIFATIGAALFSAYR"
     gene            complement(53484..55730)
                     /locus_tag="PKZ74_00205"
     CDS             complement(53484..55730)
                     /locus_tag="PKZ74_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91196.1"
                     /translation="MKNSRGHFRAGLKRTAIVLLIIAITVGWCELTHNTITSEAINLI
                     LLKNSPAYKEIELFGERMIINQGRRVNKMEDDFDRAYNHYGVNRGFFCKEYLLTPWVV
                     NLYKKHHTPPPASFDMTDFNSAYEWAYSGSVQKNPGDFYLGNEPWNWEGAVRSYDYSM
                     ISKEAAYERLGHVIHMLQDICQPDHFRRIDHPGSSFSTEELDELMKNIRKIRIIPDIV
                     WRAAGVKIPEPVPGAVGFEKLADLYEDARTHELSGLSIRDWKNVGLADGKLFNYFDSA
                     GQGSEYFRSRFGQPALDTALGLHALPVPVNYNIRAASLNTRWIPPLSVKVKIDHVPII
                     PFIVWDMPSNYPNINGYIKLSGNCTKISVEFSAGLIQLFHDIVRHPPYASKINIYQKN
                     KDGQVLTGLSYEAMWEETDDDRNRKFLKTNAEPGLFQACRQTYIEVTFGPSLSVGELK
                     QATQINPESVRVKVGVRNKSEITVDMVLKEGSDNDGSSPPVFTGSFVPPLSWAGSGDG
                     EKFQGYAKIIAEDIFNHYDYGGRAGANSGKILDALPETKARVTDFKPPYPWADYTPGW
                     DETHNDIGSFVLPPPNIEKCRIYTIGGKEFQQYIGTWDDKGCPFPEFIALDRFGGMLL
                     YDRLAAMEVEIYPEVLKIDQASVSVEIGVNGSLVVYTLNNGIRDFQVQDNGFSLKFQP
                     DLSWKSVEDAVKYGYVRIFLNDNSGHRIDANPATVPVIRSRTDWDYYEEGPDLNHQGH
                     VFIEEKPE"
     gene            complement(55720..>56960)
                     /locus_tag="PKZ74_00210"
     CDS             complement(55720..>56960)
                     /locus_tag="PKZ74_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91197.1"
                     /translation="LEQVFDEEHLVCQGVKLSELVHGQGLPEDNYTICLQAFDYLTGE
                     TVSSESPLGCSNPFTVSDLEPPVILFPVSGQDIFAGDFQNINFVWTWPPGAPNSTRFA
                     LKIIEVLPADRNINDAFRSATQPVFFEKIIASTNYLYGPSDPRLVPGKTYAFSITASD
                     PLQKSFFRNNGESEIARFNFLPEEKPIPASPIEWDVVSSRIIYYNVTSDTLSEDGAPV
                     SGQYAVKLDFRGNGDIIFQTDEKGETQTIACKMTPSGNLTWDNDRGMIPVPVLVSLSA
                     LKDPTSLIVTGDTWSKISVTDGEMNRNIDELILNFKGDFEALTVLQTGEEELICIHTE
                     VYNRIEPTDYSKDFYKNPNVTDGPFNWEKYLSGTVYFNIHLKCLERAEMILLPVFKDQ
                     APKSFKMERRIQNENKPDEK"
BASE COUNT        14947 a        13533 c        13533 g        14947 t
ORIGIN      
        1 acctcgagca ggtgttcgat gaagagcacc tggtttgcca gggtgtcaaa ctttcagaac
       61 tagttcatgg tcaggggctt ccggaagata attatacgat ctgcctgcag gcctttgact
      121 acctcacggg agagacggta tcatcagaaa gcccgctggg ctgcagcaac ccgttcacgg
      181 tatcggacct tgaaccaccg gtgattctgt ttcccgtatc cggccaggat atcttcgcag
      241 gcgattttca aaatattaac ttcgtgtgga cctggcctcc cggggcaccg aactcaaccc
      301 ggtttgcctt aaaaatcatc gaagtacttc cggccgacag gaatatcaat gatgctttca
      361 gatcggccac acagccggta ttcttcgaga aaatcattgc ttcaacgaat tatctttacg
      421 ggccatccga tcccaggctg gtacccggaa aaacttatgc gttctccatt acagcatccg
      481 atccgcttca aaaatcattt ttccggaata acggggagag tgaaatcgcc cgttttaatt
      541 ttcttccgga agaaaaaccc attccggcat ctcccattga atgggatgtt gtatccagcc
      601 ggatcattta ttacaacgtc acctcggata ctttatccga agacggagcc cccgtatccg
      661 gacaatacgc tgtaaagctt gatttcagag gaaatggaga tatcattttc cagaccgatg
      721 aaaaggggga aacgcaaacg atcgcctgta aaatgactcc ttcaggcaat ttaacatggg
      781 acaatgatag aggcatgatt cctgtacctg tgttggtaag cctgtcagct ttgaaagatc
      841 ccaccagttt aatagtgacc ggtgatacct ggagtaaaat aagtgtcacg gatggtgaaa
      901 tgaatcggaa tattgacgag ctgatcctga atttcaaagg cgactttgag gcgttgactg
      961 ttttacaaac cggtgaagaa gaacttatat gcattcatac tgaggtttac aacaggattg
     1021 aaccgactga ctattcaaaa gatttttata agaatccgaa tgtgaccgat gggccattta
     1081 actgggaaaa atacctgtct ggcactgtgt atttcaatat tcatcttaaa tgtcttgaaa
     1141 gagccgaaat gatcctgttg cctgtattta aagatcaagc gccaaagtct tttaaaatgg
     1201 aaaggaggat acaaaatgaa aacaagccag atgaaaaata gcaggggcca tttcagagcc
     1261 ggattaaaga ggactgcaat cgtccttttg atcattgcaa taaccgtggg atggtgcgaa
     1321 ctgactcata atacaatcac ctcggaagct atcaatctca tcctcctcaa aaacagccct
     1381 gcttataaag aaattgaatt attcggggag cggatgatta tcaatcaggg tcgaagggtc
     1441 aacaaaatgg aggacgattt cgacagggcc tataaccatt atggagttaa ccggggattt
     1501 ttttgcaaag aatatttgct tactccctgg gtagtaaatc tttataaaaa acatcatacg
     1561 ccaccgccag ccagttttga catgacggac ttcaacagcg cttatgaatg ggcttattcg
     1621 ggatctgtcc agaagaatcc cggtgatttt tacctgggta atgagccatg gaactgggaa
     1681 ggggctgtcc gctcttatga ttattcaatg atttccaagg aagcggcata tgaacgtctc
     1741 ggccatgtca tacatatgtt gcaggacata tgccagcccg atcatttccg gcgtattgac
     1801 catcccggaa gcagtttcag cactgaagaa ctggatgaat taatgaaaaa catccgcaaa
     1861 atccggatta tacctgacat cgtctggagg gcagcagggg ttaaaatccc ggaaccagtt
     1921 cccggtgctg ttggatttga gaagctggcc gatctttatg aggatgccag gacacatgaa
     1981 ctttccgggc tgagtatcag ggactggaaa aatgtcggat tggctgacgg aaaacttttc
     2041 aattatttcg attcggctgg ccagggctcc gaatattttc gctccaggtt tggccaaccg
     2101 gctttggata ccgccttggg gcttcatgca ctccctgttc ctgtaaatta taacatccgg
     2161 gccgcttcac taaatacaag atggattccc cctttatcag ttaaagtaaa aatagatcat
     2221 gttccgataa tcccctttat tgtctgggat atgccgtcga actacccgaa tattaatggc
     2281 tacataaaac tttcgggcaa ttgcacaaaa atttccgtcg aattttccgc cggcctgatc
     2341 cagttatttc atgatattgt caggcatccc ccttatgcca gtaagatcaa catatatcag
     2401 aaaaataaag acgggcaggt gttgaccgga ttaagttatg aggccatgtg ggaagaaaca
     2461 gacgacgacc ggaacagaaa attcttgaag acaaatgctg aacccggact attccaggca
     2521 tgccgtcaga cctacattga agtgaccttt ggcccatctc tttcggtggg agaattaaag
     2581 caggctacgc agatcaatcc ggaatcggtc agggtaaaag taggggtcag aaacaaatct
     2641 gaaataacag ttgacatggt attgaaagag ggcagcgata atgacggatc atcaccgcct
     2701 gtttttacag gaagtttcgt tcctcccttg tcatgggctg gttcaggtga tggagaaaaa
     2761 ttccaggggt atgcaaaaat aattgcggag gatatattta atcattacga ttacggagga
     2821 agggcaggtg caaatagcgg caaaattctg gatgccttgc cggagacgaa ggcccgggtg
     2881 actgatttca agcccccgta tccatgggcg gactatacgc ccggttggga tgaaacacac
     2941 aacgatatcg gatcatttgt gttacccccg ccaaatattg agaaatgcag gatttatact
     3001 attggaggaa aagaatttca gcaatacatc ggcacatggg acgacaaagg gtgtccgttt
     3061 cctgaattca ttgcactgga ccgtttcgga gggatgctat tatatgaccg cctggcggca
     3121 atggaggtag aaatatatcc cgaagtcctc aaaatagacc aggcatcagt cagtgttgaa
     3181 atcggtgtta acggaagtct ggtagtttat acgcttaaca acggcatcag ggattttcag
     3241 gtgcaggaca atggtttttc cttgaagttt cagcccgatt taagctggaa atcggtcgaa
     3301 gatgcggtaa aatacggtta tgtgaggata ttcctgaacg acaactccgg ccataggata
     3361 gatgcaaatc ctgccactgt tccggtgatc cggtccagga cagattggga ctattacgaa
     3421 gaagggcccg atttaaatca tcagggtcat gtcttcattg aagagaagcc tgaatagcaa
     3481 atacagatgt gatttcggtt ccccggccgg ttttatcatc tgtacgcgct gaacaatgcc
     3541 gcgccaatag tggcaaaaat gatgatgtaa agcacgataa caatagccaa ccagatgagg
     3601 gatgcctttg cccagttggc tttgctcgga tgggtgctgc tgctgaaccc ccagacgaac
     3661 agcatgatga tgttgacgag cgggatcgca gtaatcaaaa tggtcacgaa ccagtcgccg
     3721 accgggatcg gttgctgatt ctgcatggca ttgtaattct ggtaaggatt ctggttctgg
     3781 ttctggaatt gggtctggtc ttccatttta gtttggattt tggttgtcct actcgtgtgg
     3841 cttttcggtt ccgccccgtt tgatggctgg tttccgggct ccaggtatat aaatttattt
     3901 taattcagga aagaaatatc ttgacagggt aaatttaagg aaaagaacaa ctgaattcaa
     3961 tacgaatcat tgaaatattt tcaaattcag ggaaatacag gatcattcta accgattata
     4021 cttatttcgt atctttatta ccaaaatacc agaccatgaa attccggcgg tactttgcgg
     4081 tatgccttct gatcgtgacg gcttctcctc tcttcgccca gcccggcggt tattatcctc
     4141 cgccttcggt tgtcgttttc cagaacgata cccttaccat cttcccgccc gattcgcttc
     4201 ccggagagcc ggtagagctg ctttcttaca atatcctggt cgatgacgaa ttttacgata
     4261 accttccggt cctcgttccg gccgaacctg ccgtctttat cttcgatata acaaccctca
     4321 ccccgggaaa ccggagcttt tgcgccaaag cggtctacaa tgaatggatc agcgaaagtg
     4381 cctgcgatac tgcacaaatt ttttatggga tggagctgcc ggtactcgaa gactggtcgt
     4441 cgggcagctt tgaaacgctg caatggacga ccgatagcga aaactggatt atcgaaaacg
     4501 atgaaggcaa tcctgcacct tccgcagcat tccttggcaa tccggcagtg accggttatg
     4561 aaatcgccct ggagagcttc cccatgacgg ctgtggggat gacgaacgga agaatctggc
     4621 tcgatttcga aatcaaactg gatgcaaacc aatcgacggg tacagaaaaa ttattcttgc
     4681 aggtgtggaa ctggaccaac gaaacctgga atgctatatc tgaatatagc aattttgacg
     4741 gcagttttga ttggatcagt gagcggattt taataacagc aaagaataaa gttttcaagg
     4801 tccggttcct ggctaccggt gaaaattcgt ccgcaataaa tagatgggca ttggataata
     4861 ttcacattta tcgcacctgt gatgggctct gggatgtgtg gctggaagaa aatccggatt
     4921 acaatactct tgaatgggat ggttatggag gatggggatg gtttgatggc tggttcaact
     4981 gggatgatgg cgtattcagc ggtaattcaa tcggtttggg tgaaccggct gaatttgagt
     5041 ttgcctcaag gtgggaaccg gctcagttga tcattttccc tggagccgta atcacggaag
     5101 ttgcttttgt ccctgcagaa gtaaacgcgg attaccggat caggatctgg aacggaagcg
     5161 cacccgactc cctgcttatc gaccagcctg ttcaataccc ggtgataggt gaatggaact
     5221 atgtgacact ggttgatact gtaactattg atgtaacaaa agatctctgg gtcggttatt
     5281 atgtcaatgc gcagaccggt taccctggag gagttgatga cggtccggct atcgatggat
     5341 ttggaaacat gatctactat caggaccaat ggcagacatt aaaagatatc agtgacgacc
     5401 tcgatttcaa ctggaatatc gcatgccatg taattcccgg gctgccattt ccgaatgata
     5461 aatggcttta taaaatctac cgtaacaccg atggcggcga ttacctattt ctcgatacga
     5521 ctttttcatg gaactatgaa gatcatgaca ttatccttga agaaaaatac tgttacaaag
     5581 tatcggcagt ctggactgag aacaacgaca catgtgaatc cgacgataac cccgaagtat
     5641 gcgaaacgct ttgcctgggt gtcgattcac cgcaacagga taataaggtg aaaatttatc
     5701 ccaatccggc atcaaactgg ctttggatcg aatctgaaga tttaatccgg gagatcaaac
     5761 tgtttaattc gcttggagag acgctgatga atctggaaat tggaaatttg aaattcgcag
     5821 tgtattgttc cagtttgccg gcagggattt atgttatcga agtaaagacg caaactacca
     5881 ccttccgctc aaaattcctg aaaatcgatt aatgtcctga acgccacctt cccgtcttcc
     5941 ctggttccct gcaccacaac cgagttccct tccgccttgc cggtcgagat catcatcatc
     6001 tccccggcca tgacctccga taggtaattg atggtcagcg tacggatgcg atgctccttg
     6061 tgccattcct ccggcagggc atccacgctc cagtcgagat aacggatgtt gttgacatgc
     6121 ccgttctggt cgacttcgct gaagcgcacc ttgcggggtt cggtcgtcgc aatctctttc
     6181 accggccgta gcagcggggg attgcagtcg agcacgtcct ggtgggtggc caggtgcagc
     6241 accggtttga ccaggtcgac gctcttcggc tttctcgttt cctggtcgag gatcatccac
     6301 gccgaggtcg ccgccccgat cacattcccg ttgtgatcca tgatcttgaa gtccctgtaa
     6361 gcaaataact tatccacccc tttcggccat gtttccacga tgattttctg accccaaacc
     6421 ggctggccga tcatccggat catcaggctg atgaccaccc agaattcgtt gcgtttcctg
     6481 gcatcttcgt agccaaagcc gagatggttg gcatgcctcc atgccgattc ctgcaggtaa
     6541 ttggcaacgg ccgacatttt caattgcccg ttgcagtcgg tgtcgtacca gtagatcggg
     6601 tattcctcgg cccagatttt cttcagtttg ttcatcaacg ctttacgatt attgcaggca
     6661 aatttattaa ttaaccacaa agagacacaa aggacttcac gaagtaacac aaagggttta
     6721 atttggtaat caattacttt gtggcctttg tgattccttt gtggccttgg tggttaaaac
     6781 tttttgagtc atctcagcat aaaaaacccc ggaaagccct gttaaaagcg atccggggag
     6841 gagatatatg gggaacagtg gtgtgctgct acattttcga cagctcgata tctaccgtta
     6901 cttcaatggt ttcggcaatg tttttgacaa ccgcacccgg gattttgatc gaataatcgg
     6961 caggcttcac gttgaatttg ctgatcccgt ggatcttatc cccggccttg acggtaaacg
     7021 tccctttttc gctgatcttc ttcgttacgc cgtgaatggt caggtcgccc tcgatcgtcg
     7081 cttcataaga cccttctttt gagaaatcaa cagcagtaag attggtcact tttccgacaa
     7141 aggtcgaatt cgggaacttg tcgctctcca tatagttctc gttgaaatgc tcctgcatga
     7201 gtgctttttc gaactggaac gacttgataa gaactttgaa taccaggtcg ccggtttggc
     7261 tgtcgagggc agcatttacc tggttgttag tagcctcgat agtttcgatg ggggtttcgg
     7321 agtagaattt gatattcccg ttcttggtca tgtatttctg ggcctggacg gtgaacgatg
     7381 cggttatggc tatagccgcg atgacaagga ttttaagtga ctttttcatt ttatttgatt
     7441 cttttgaatt aataatcgat tgaattttta acgtatgatg gaacactgct ccaggacgac
     7501 atcggtatcg ttgtagccgg tcaggtatcc tttgatcttt acttctgttc ccggtccgat
     7561 ggctttcagc gcctgggcat ggttgccgag cataacacag cggatccctt cgtcgccgaa
     7621 catcccctgg ttaaagacaa ataccccggt aacaaggctt tcagctaaat cgatcttatc
     7681 gagtttacca ctcagcagca ccactttgcc gttgaattta ccttcggctg tcgtccggtc
     7741 cgactgaaag ctgttataca ggtcggtggc agagataata aaggccggtt tggctttctc
     7801 atagttttcg tgaggcttgt tgatgacgaa tttataaaca aggcctgcgc tgatcacccc
     7861 tatgacaaac agggacagaa tgatggttat gattgttctt tttttcatgg tttctttatt
     7921 ttttgcacaa aagcgctata aatctctatt tactaacact attaactctg gaacctggaa
     7981 cattggaaca ctggaacttt ggaacactgg aacacttata gctttccgaa tctcagttct
     8041 tccgcgctcg tcaggttatc gtcctgcagc cagatctccg tatcggtcat tttaagtatt
     8101 ttccagtcgt cggtcaggtc atccatggct ttgtttcctg taatggtaat gacaaatttg
     8161 ttactggagt tatcgtcgtc gcccggttcg cctaaggtcc aggtgccggt ataagttgcg
     8221 gagcccgtaa cagcttccag gatattcccg gtattgaaat taaaagtgta cccgttgtaa
     8281 tcgctggtct cgtctttgcc gctgtcgaaa tagtaggtta ctttccagtt accggtcacc
     8341 tgtccggtgt tattggtatt ggaaggatta tcgtcgttgc tcttggagca ggaagccagg
     8401 ctgatggtca gtgcgaggat gaaaagtttt gctgtagtct tcatggtgtt tttgtttgat
     8461 gaggcaaagt aacgtcgaac cgagggcgag ttccaaataa tattggatga acgggaaaaa
     8521 gtgagacttg aatggggaag ttgggcagtt ggacagcctg tcccgagtag agcgagggat
     8581 ttgggcagat ggactaacct ccccgcagcc actccctgaa ctcggccgac cgctcggtgc
     8641 tggtgataga ctccagctcg cagggcggtt tcagcttgat cttgatgcgg gctttggagt
     8701 aggtgatcat ggtttcgatc gaatcgaggc tgatgataaa ggcgcggttg attcggaaga
     8761 accgcccggg atcgagttgc ttctccagct gatcgaggct caggtccatc gggtagcggt
     8821 ctttgccgaa ggtaacgccg aaaacgatct tttcatctat ataaaaataa gccacatctt
     8881 tgatctccac gatcttgatc tgctgcccca accggatcac cacccgcctg aggtattctt
     8941 tctcctgttt gcctatcagc gccatgagct gcgataaatc gacctgcttt tccgccggct
     9001 tcaccttttt gaatttattg atcgcctcag ccagttcttc cttcctgacg ggcttcagca
     9061 ggtaatcgat gctgttcagc ttgaaggcct ggatggcgta ctggtcgtat gccgtggtga
     9121 agataacggg gcaggaaatg ttgatctgcc tgaagatttc gaaactgagc ccgtcggcca
     9181 ggtggatgtc cgatagaatc agatcgggct cggggtgatg gccgaaccaa tccatggtgg
     9241 aggtgatgct gtcgagaatg ccgaggacct tcacctcggg atccatctct tccaccatct
     9301 ttttcagccg ctgggcgctt gccggttcat cttcaataat aagaagtttc attttcttta
     9361 tttcatttaa ttaaccacaa agtacacaaa ggtttcacaa aggagcacaa agtgattgac
     9421 tgccggacta aggccttcgt ggatctttgt agtataataa tcatttgatc aagggtaaaa
     9481 aaatcctgaa aagcagttcc gatatctcaa ttatgacctc tttatcggta atcagtttat
     9541 atcttttcct gatattttca agtccgatgc cggtggagac ttcgggagtg agtttgggct
     9601 ggagattatt ctctacaacg atcttcccat catcttcgta aactttaata aacaaaggct
     9661 tactcatcga tataacatta tgcttcacag cattttcgat caagatttga agcgtcatgg
     9721 gcgggataaa tgcctttttg agctcatcac caagctgaat atccaggtaa aggttatctc
     9781 cataacgttt cttcaaaagg aagaaataac tttgtatgag tgtaagttct tcatccaggg
     9841 tgatcagctc ctggtctttg tattgcagga tcgtcctgaa aaaatcagat agtttttccg
     9901 tatattgtac cgcctgttca ggattatctt cgatgaggga gatcagggta ctgaaactgt
     9961 tgaagagaaa atgtggattg acctggtttt tcagcacctg gaactcaaac tcgatcttct
    10021 cttttttctc ctgttctctc tttctgagcc tgttttcacg cattttaatg tatagaaaga
    10081 taagcgccgc caggataatg accagcagga tgataaacca gggttgtatc cagacaggtt
    10141 gccttatcct gaagctgtaa ctgacttcgc cggcattgac aaaagaccgg tcgagcgagg
    10201 atcttacttt aaaggtaaat tcaccgggcg gaaggctgga ataggtcacc tggcggttgt
    10261 aagtatcgtt ccagcccagg tcgtaccctt cgagcataac ctggtaattc actctttccg
    10321 gattggaaaa ccaaaggcct gcataccgga atgaaatgtg gttgtcggca taaccgaaca
    10381 cctggccggc cttcaactcc cggggttcca ggtaaaccga catctcctcc agaaccgtga
    10441 cgggcatata agaaaccgcc gaccccggcc ggtaccggat gatccctttc cgtgtcccca
    10501 gccatatatt cccttcgcgg tcgcggctga tcgtgttgag atcgggctgg atcccttcaa
    10561 atcctttgtc accgccataa tgagcgatgt ttcccgttgg aatgtgtagg atatccaggc
    10621 cattctcatg aacggccaga agttcatcgt ctgctgccat gatcccggtg aaggaaatgc
    10681 tgctcagacc ctcgtcaagc ccatagtgaa tgaagttctt cccatcgaat ttaaacaatc
    10741 cgcccgatgc cgatccggcc cagatattgc catatttatc ccccgtaagg gaatagatca
    10801 catcatcggt aaggccattg ttttcattgt atgaagacca tccgtcctgc gtcctgacat
    10861 tgatcccgtc tccgtcggtc gccagccaga tcctgttttt atcatcctca taaacataat
    10921 agatgaaatt attgaccagc ccgttttcat aattgtatga ccgggcctga taagatcttc
    10981 cggaagtatt cgggtcaaga atgatttccg aagctcctcc cagggttgcc atccacaagg
    11041 tgtcgtgatg ccgggatatg gataaaacat tgttattgac caggttatcc ctttcggtaa
    11101 ttcgatcaaa tgtgccattt gcaggattga gcctgatcgc cccgtagtta aaagtacctg
    11161 cccagatgta gccaaagcgg tcctggttaa ggcaggtgat tttcagtccc ggccctttaa
    11221 acggacccgg gtaagatttt acatcgcccg attcgacggc cagggagaac aacccgcggt
    11281 cgtcgctgaa ccacagcagg ccgggacgga actcgtcggc atggatggca tggatattcg
    11341 ccagcggaat cccgccaaca tcatccaggt atcgaatcct gttcccggtc gaccggaaca
    11401 gcccttcggc agaaagtatc cagatattgc cttcccggtc ttccagcaag tcataaattt
    11461 tgccggtctg aaggtcagcg ggtaacagat taatcattcc ccgattattc tcattcagac
    11521 tgatctccag taattcaccg cttgaagtgc ctgcccacgc cgatgcaccg gaaaccgtca
    11581 ctgcgctcac cggaccctct tcccaggcat gatctgccgc aggctgaata aattgccgcc
    11641 cgtcgttcgt caaaaaacca atccctccgt cctggaaacc cagccagagg ttgccatcac
    11701 tatcctcttt gaccacagtg accattatat ccggcaattc accggacgcc gcaagcggat
    11761 tgactatttt ctccccgttt ctgtaaatac acgaaaccag cccgccatcg gtacccgcca
    11821 caacatatcc atcgctcaca gattcaaggt catgcacata atcgtcggga aggccgtcgt
    11881 cacggtttat attgaagacc cgggtgccgg agtaaaaata gatcccttcg ccggctgtcg
    11941 accaccagat actacggtct tgggctacca ggatatcggt gacagccaca ccggaagtac
    12001 cctcttccgg attaaagagg ttgatccggc cctgatccaa ccggtatatt ttaccgtccc
    12061 tgcacccaat ccagagatcg tgatcctggt caaggtgaag tgcagtcacg tggaaatcag
    12121 ccgtatccgg taagggggta aaagatttga actgaatgcc gtcgaaacgg aacaggccct
    12181 gtccggaacc cagccagatg tatccatctg catccataac agctttttta aagacaggtc
    12241 cttcccccgg gacggtgatc ctgatcttct caaagcccgg aagttgtgag aatcctgaat
    12301 gggcaatcag gaagataaaa gccgtgagcc aaaacaaagg actccacaat ctccttattt
    12361 tctgaccatt aatcattgct ttttaccagt tcggcagtga ctgaaacgtc aatactttcc
    12421 gcaatcttct ggttaacgac ccggggtatc cttatctgat ggtcttccag catcacgcta
    12481 aaatctgcct tcaggctgat cttcccatct accacccgga tcgttccttt aataatcctt
    12541 tcctgttcaa cgccgtgaat attcagcatt cctttggccc tgaccgtttg ctccagtgaa
    12601 gagttcaggt cgatatcttc aatgatcttc cctttaaaag ttgaggcagg aaaagactga
    12661 acctccaggt aattttcata aaaatgctcc tgttgcagcg ggctgttaaa ccctttaaaa
    12721 ctcttattat caatggaaaa ggcgaatgtt ttcttccccg gatcgatggc gccctgcacc
    12781 tctgcagatt cggccctgat gatctccagc ggggcattgg atacaaagga agccgtgcca
    12841 ttctttgttt tataaacagg ctggcagaag gaattatttg ccagtaaaac aatgatgatg
    12901 aataagatat gtttgatcac cgtagagatt ttaaagcgaa gataatatat tcctgataaa
    12961 agttgattcc gtaatgaacc gatgtatcag cggattgttt agaccagatt aaacagttta
    13021 tacttttgca ttcgattaaa accaaactat gaagatattg ttcatcattc tcattgtaat
    13081 tctgatcatt attctgggtt cccttttact cggatggttg ttttcggcgc ctgtttacca
    13141 cggtcctgtc agcgaccatt tcgacgggaa gagattcatc aatccgggtg gaaagaaagc
    13201 gggagggctg aaagatgtca tcaaatggtc gagggaaagg caacggggtg aatggaaaga
    13261 gatcaccgaa gtgaattatg gccctgcccc ggaagaaaga gtcgaaggtg attctattgt
    13321 tgtgactttc gtcaaccatt ccaccttcct gatccagacg caggggttga atatcctgac
    13381 cgatccggtc tggagcgaac gggtcagccc tgtgacgttt gccggcccga agcggatgcg
    13441 ccagccggga atccggtttg aagaacttcc tcctatagac attattttat tgacccataa
    13501 tcactacgac catttggata tcagtacctt aaaaaaattg acaagccgtt ttaaaccggt
    13561 gatatacacg ccgctcgggg tgggccttta cttaaagaaa aagggcattg acaatttcag
    13621 ggaaatggat tggtgggacg aattgacaac gccaaacggg ttgaagctgg tttgtacgcc
    13681 ggcgcagcat ttcagcggcc gggggatgtt cgacagggac aggacgctct ggtcaggatt
    13741 tgcattacgc accgaaaacg ggagcatata ttattcggga gacaccggct atggagagtt
    13801 tttcaacgaa atagccgaac ggctctctcc gatccggctt tctttccttc caatcggggc
    13861 ctataaaccg cagtggttca tgtcgccgat ccacacatca ccggccgatg cggtgaatat
    13921 ccacaatatc ctaaaatccc ctcaaagcat cgggatgcat ttcggcacct tcccgctggc
    13981 cgacgacggg atatcagatc cggcgaaaga gcttaaggaa gcaaaaagca gagagggaat
    14041 tttagatgag agattcctga tacttgaaga agggcgagca tatagttttc attgaggtgt
    14101 ttcttcgaga tggcatctca atatgcaaaa gaaattttct atataagctg atttgcgggt
    14161 gaaaagaccg gatcttattt gattttgaga tgccatctca aagaattaga tttcatattc
    14221 taaactgctt atttataatc acatatcgct tccaggaact tgtaatcctg atgccatcta
    14281 aaaaatattt ttaagggtcc gggtattttg tagtcaaatt acggattaac aattgagaat
    14341 tcatcctttt gttaaaccta taatcaattt acaatgaaaa agcaatcttt aaagacacct
    14401 ctaattgcgg ggaagtatta tcatttattc aacaggggaa ataatagaga tagacttttc
    14461 tatttcgatt gcaattatga cttttttatt aaaaagtact tctactatat ggatccggtt
    14521 gctgaaacat tttgctattg tctgttacca aatcattttc attttttaat tcgtataaag
    14581 gattcagcaa ccgattcaag gtatgtttca aatcaactaa ggaagctgtt tataacttat
    14641 gcaatgtata ttaatcgtca agaatgcaga actggctgta ttattagtaa aaacttcagg
    14701 cgaattgagg taaatcgcga cgaatacttt attagcctag tgcgatacat ccatttaaat
    14761 ccgataaagc ataagattac taacgatatt tatacttata gatacagtac tttttacaaa
    14821 attctccgca atcaaatcaa tgattttgag tctgcggaat taattaattg gtttggagga
    14881 tactccaatc ttattgaaga tcatctgatt ataaagaatg ataaaaaaat taatggtttt
    14941 aaactggaag actagtcttg gattttactg agtaataagg ataataaact tggagttcag
    15001 aaggttgatt ttcttttaaa tagaaataat aagcaccatt ttatgagatg gcatctcaaa
    15061 tcaatagatt tcttctttaa ataatttatt atgtgtttgt taggaagcat tttcatgatg
    15121 tcagagatgc catctcaatt acttttcaac aatccgaaat atctttatca aaacatatat
    15181 ttttagatcc gggaaatcat ataattgaat atcaactgcc tggtctatga ataaaacggt
    15241 tcctgcaacg cttatcctgg atgatggcac ggaattccgc ggctattcct tcggcgccga
    15301 acaatcttca tccggcgagg tggtcttctc caccgcaatg aacggctacc cggaaagcct
    15361 caccgacccg tcgtacaaag gacagatcct cgtagccacc tacccgctta tcggcaatta
    15421 cggggtgcct gaaatgcgga tgaaagaagg catgacggaa ttcttcgaat cggataaagt
    15481 tcagatctcc ggcctggtca tttccgacta ctctttcagt tacagtcact ggaatgcagt
    15541 aaaaagcctg tcggactggc tgaaagaaca ccgggtgccc ggcattgccg gtgtggatac
    15601 acggaaactg acccggatca tccgggaaaa aggggccatg ctggggaaaa tcgtagtgca
    15661 ggatgatgta catttttacg acccgaacaa agacaacctt gtcaaccagg tttcggccag
    15721 ggaaaaaata acctacggga atggccgtcg tcgtatcctc ctggtggatt gcggcactaa
    15781 aaacaacatc atccggtgcc tgctgaaaag agatgccact gtgcaccggg tcccctggga
    15841 ccacccggcg gatcccgcgg aatacgacgg cattctggtc tccaatggtc cgggtgatcc
    15901 taagatgtgc aaagccacca tagaatcggt gaagaacctc cttgatggcg atctgccggt
    15961 ctttggcatc tgcctcggct cacagatcat ggccctggcc gcaggcgccg atacctacaa
    16021 gttgaaatac ggtcaccgga gccacaacca gcccgtaatc atgaaagaga gccaccggtg
    16081 cttcataact tctcagaacc acggctatgc tgttgataca gagacactcc cggagggttg
    16141 gaaaccctgg ttcgagaatc tgaacgacgg tacctgcgaa ggcatcatcc acagctcaaa
    16201 acctttcttc gccgtacagt tccacccgga agcctctgcc ggtccggtcg ataccgaatt
    16261 cctgttcgat cattttatgg aaaaagtcag ggaatatgaa gcacgccatt aaaaaagtcc
    16321 tcctgctggg atcaggtgcc ctgaagatcg gcgaagccgg cgaattcgac tattccggca
    16381 cccaggcgct caaagccctt cgcgaagaag gtattgctac cgtcctgatc aatcccaaca
    16441 tcgccacggt ccagacttcc gaagggatgg ccgataagat ctatttcctc ccggtaaccc
    16501 ctttcttcgt cgaaaaagtc attgaaaaag agaatccgga tggcatcctc ctgtcgttcg
    16561 gcgggcagac cgccctgaac tgcggcgtcg ccttgcacca gtcggggata ctggagaaac
    16621 acggggtcag ggtattggga acacccgtca gcgccatcct cgacacggaa gacagggaac
    16681 tttttacccg gaaactggat cagatccatg taaaaacacc gcgcagcatc gcggtgaccg
    16741 gtgtggatga ggcggtgcag gcggcggcaa ccctgggtta tcccgtgatc gtccgctcgg
    16801 cttttgccct cggcggactg ggcagcggct tctgtggatc ggaagacgaa atgagggcgc
    16861 tgacacagaa atccttctcc tattccagcc aggtgctggt cgaagaatcg ctgaaaggat
    16921 ggaaagaggt ggaatacgag gtggtacgcg acggtttcga caattgcatc acggtgtgca
    16981 atatggagaa tttcgatccg ctggggatcc acacgggtga aagcattgta gtagcacctt
    17041 cgcaaaccct gagcaacagg gaataccaca agctccggga actatcgatc cggatcgtca
    17101 ggcatatcgg aattaccggt gaatgcaacg tgcagtacgc ccttgatccc aactccgaag
    17161 actaccgcgt gatcgaagtc aatgcccgcc tttcccggtc gagcgccctg gcgtcaaaag
    17221 ccacaggata cccgctggcc tttgtggcag ccaaaattgc cctgggctac ggcctgtttg
    17281 agctgaagaa ttcggtgaca caggatacat cggcattttt cgaaccggcc ctcgactata
    17341 tcgtcgtgaa aatcccccga tgggacctgg gaaagttcca tggcgtttcg cgcgaaatcg
    17401 gcagcagcat gaagagtgtg ggggaagtca tggccatcgg gcgcaccttc gaagaagcca
    17461 tccagaaagg gctcaggatg attggccagg ggatgcacgg gtttgtcggc aacaaagaca
    17521 tcgaggatat catcatcgac aaagagctgg ccgtgcccac cgatatgcgc atctttgtca
    17581 ttgcgatggc gctgatgaaa ggctacagcg tggaacgcat tttcgaactg acgaagatcg
    17641 accgctggtt cctggaaaaa ctgaaaaaca tctgggacat tcgcaatgaa gtgataacct
    17701 acaacaccct ggaagaactg cccctcgccc ttttgctgag atcaaaacag ctcggcttct
    17761 ccgattttca gatcgccagg ttcattttta aaagtgaccg ggatcacatt gcggatgacc
    17821 tgctgaaagt gcgaaattat cgcaaagcaa aaggcatcct gccttatgtg aagcaaattg
    17881 acaccatggc agcagaatac ccggcccgga ccaattatct ctatttaaca tacaatggca
    17941 acgagcacga tattggcttt tccgcaaaga atgttactac tccaacgact aaagccgttg
    18001 gttccgagga tggattaaat ttggaaccga cggctttagt cgtcggaaga aaagaacagc
    18061 gtgaaccgac ggcttcagtc gtcggactac aaaaagagga aacaccagaa accgttgtcg
    18121 tccttggttc aggcgcctac cggatcggca gcagcgtgga attcgactgg tgcggggtga
    18181 atgcgctgga atgtgttaat aaggaaggat accgttcggt gatgatcaac tacaaccccg
    18241 agactgtcag caccgattat gacctgtgcg accggctcta tttcgaggaa cttacctttg
    18301 aaagggtgat ggacatcctc gatcttgaaa accccctggg cgtgatcgtt tcaaccggcg
    18361 ggcaaattcc gaacaacctg gcgattcggc tctgggagca ggaggtggcg attctcggca
    18421 cctcgccgca gtcgatcgac aaagctgaga accggcacaa attttcggct atccttgacg
    18481 aacttggcat cgaccagccc cggtggatcg agtcatcttc cgtaagcgag gtataccggt
    18541 ttaccgatga ggtcggtttc ccggtcattg taaggccgtc gtatgttttg tcgggtgcgg
    18601 ccatgaacat tgtgtcgaac cgcagcgagc tcgaacattt cctggagatg gcggcggagg
    18661 tgtcgaaaca gtatcccgtg gtggtttcca ggttcattga agacgcaaag gagatcgaga
    18721 tggatgccgt agccgcgaac ggggagatca tttgctatgc gatctcggaa catgttgagt
    18781 tcgccggcgt tcactcggga gatgccacca tgctgtttcc gccacagaaa atctatgtcg
    18841 aaacggtgag gcggatcaag cggatcagcc ggcagatcgc gcaacacctg aaaatttcag
    18901 ggccgttcaa catccagttc ctggccaaag acaacgatgt gatggtgatc gagtgcaatc
    18961 tcagagcctc tcgcagcttc ccgttcatct caaaagtact gaaaaccaat tttatcgaaa
    19021 ccgcaacgaa aatcatgctg ggaaaaacgg tggccaaacc ggataaatcg ttgttcgatc
    19081 tcgattatgt cggcgtcaaa gcctcgcagt tctcattctc ccgattgcaa aaagccgatc
    19141 ctgtgctggg cgtcgaaatg gtttccaccg gcgaggtcgg ctgcatcggc gacaattact
    19201 acgaagccat tctgaaatcg atgttgtcgg tcggctatac gatcccggag aaagctgtgc
    19261 tgtattccag cggcccgtcg cggtcgaagc aggaattgct caccagcgcc cggatgctgc
    19321 aggagaaagg tctgaccatt tatgccaccg gcggcaccca cgatttcttc atcaaaaacg
    19381 ggattaattc gatcatgctg cactggccgg gcgaggataa aaccccgaac acgatggaat
    19441 acctgcgccg gaagaagatc gacctggtga taaacatccc caaggacctg agcaaaagcg
    19501 agcttgacaa cgattaccgc atccgccgca gtgccgtcga tttcaacatc ccgctcatca
    19561 ccaatgcccg gctggccagc gcatttatct actcgttctg caggatcggg atcgaggaga
    19621 tcgggatcaa ggcgtgggga gagtacgagt gaggaacttc gatttacgat ttacaattta
    19681 cagtttacaa tctttgaagt ggattatgga atatgatcat cggttattct tattattcag
    19741 tgtgttaatg gatttttgaa aaatagcaat gagatcctga cattctttta aaagatctac
    19801 tattctaaga ggattattac aaattacaga gttactcatt attgttatat tcacttgagt
    19861 ttctctcagt tctttgagta caaggctaag tttatgagta aaatccgcac gtgattccgc
    19921 actctgtgct tcaccataat ttaatgcagc cgagctggca gatcttacaa tctgtttagc
    19981 aagatgaaaa gtataatcat caaaacgaca ttccttacaa agcgtaataa ttgccgatgc
    20041 aaagctaatt agtcgctttt ccagttcaat ccggttcata ttgttttttc taattaatcc
    20101 cattttctca caataaatac ccggtttagt taaaaaaatt tttctattca ttaatcttgt
    20161 ggtagttaca attgtcaaga attacaattt agagattact ttttacattc tgcagagtac
    20221 aatcttcaat gcaaaattca gattatagat tgtaaactgt aatccgtaaa ctgtaaatca
    20281 tttattattt ttaccacatt caaataattg aaatcttgat cggtcagaaa tacaacaaca
    20341 accttcagta cttactgata gtagtcgccg gggcgctcct tttcctgcct ttcctgggca
    20401 acgtcgccct gttcgactgg gatgaggtca attttgccga gatcgcccgg gagatgatcg
    20461 ttacgggcga ttacctcaac gtgcagatca attatcagcc attctgggaa aagccgccgc
    20521 tgttcatctg gatgcaggcg ttgtcgatga agctttttgg catcaatgaa ttcgccgccc
    20581 gtttccccaa tgctatttgc ggcatcacaa cgctgctggt catcttcgcc atcggaaaaa
    20641 aaatccataa tgaaaaaatg ggcctcacct gggtgttggt ttacgccggt tccatccttc
    20701 cgttcttcta tttcaaatcg ggcatcatcg atccctggtt caacctgttt attttcctgg
    20761 gcatatattt tgcaaccatt tccatcgaca gatcaagagt tcaattgttc aatggttcga
    20821 ttgttaatga acccgacatc tcgtcttaca atcctaccgt cttacagtcc aacagtcata
    20881 aattcctaca ttcccctggt ctccttgtct ttctttccgg tgccgccatt ggtctggcta
    20941 tcctcaccaa aggccccgtc gcccttctga tcttcggact ggttgccata gtcttcccgt
    21001 tctctttcat cctgaccaac atcaaaaaat ccacaacttc acagaatcaa atctccaaat
    21061 ttccaaattt ccaaattatc acaaaatccc tctctctttt cgccatcggt ctcctcctca
    21121 ccggcggctc ctggttcctt ctcatgatag ccaacggcca cctcgatgtg gtgatcgact
    21181 tcatcgtata ccaggttcgc ctgttcagca ccaaagacgc aggccacggc ggtttcccgg
    21241 gataccacgt ggttatcctg atgctgggtg tcttcccggc atcggcattc tttttcggag
    21301 gactaaaggc cgtcccggaa gagaatttcc tgcaaaaaaa ctggcgccgg gtgatgatca
    21361 tccttctcgc aaccgtggtg gttcttttcg agattgttca gacaaagatc gttcattaca
    21421 gttccctggc ctatttcccg ctgacctacc tggctgccct ggccctttac cgggccagcc
    21481 tttcagagag agcattttcc cgcagttccc gcatcatcct gtggaccctg gccatcctct
    21541 ggatgacggt aatcgcagga tttacactgg ttggtctgaa caaagtgtgg ctgatgaaca
    21601 gtggcctgat caaagacccc tttgccgtcg ccaacctggc ggccgacatc cggtggacag
    21661 gcttcgagac tctggttgcc ctggtgctga tcccgggaat tgccggcttc ttcatcctga
    21721 aaaaaccttt tcataaaatg attgcgcttt tcagcacctc cctggtcttt accttcctca
    21781 ccatgaccat tttcaccggt aaaatagaag gatatgttca gcgtgctccc atcgaatttt
    21841 atgaaaaaca cagtagtgag gagtgttata tcaataccct cggttttaaa agttatgccc
    21901 acctttttta tggaaagaaa atgccgccgg tcaatccgat ttcttatgat aaagaatggc
    21961 tgctcaacgg aaagattgat aagaacgcct attttgtata taaaataacg aagaaagaag
    22021 aatattcccg tctttatcct caactgaccg ttttatacga gaagaacggt tttatctttg
    22081 cggcgcgata tcctgaataa taaaatgcag gaatatgatt tatggcaaga agataactgt
    22141 cgtactaccg gcatacaatg cggaaaaaac ccttgaacaa acctaccggg agataccgtt
    22201 cgatatagtg gatgaggtga tcctggtcga tgataaaagc cgggataaca ccgtggaggt
    22261 ggcaaaacgg ctcgggatcc atcatatcat cgagcaccag gaaaacaaag gatacggagg
    22321 aaaccagaag agctgctacg ataaagcgct cgagatcggt tccgacatcg tcgtaatgct
    22381 tcatcccgat taccagtaca cgccaaagct tatccacagc atgtgctacg tcattgctaa
    22441 cgacgtttac cccgttgtcc tgggatcgcg tattctcggg aaaggtgctc tcaggggcgg
    22501 gatgccgctt tacaagtaca tatccaaccg attccttacg ctggcacaga acatgcttat
    22561 gaagcaaaag ctatcggaat accataccgg ctacagggct ttttcgaagg aggttcttca
    22621 gaatatcaaa tatcagcata actccgacga ttttatcttc gataaccaga tgctggccca
    22681 aatcttcttt gccgggtatg agattgccga agtcacctgc ccgaccaaat atttcgacga
    22741 cgcctcttcc atcaacctga aaagaagcat gaaatacggg ctgggcgtac ttggggtttc
    22801 attccggtac ttcctgcata agcacaggat tgcccggtac aggatcttct aaaacagaga
    22861 tccctcactg cgttcgggat gacgatgcgt cacgaacaca gtgagggatc ttactcaaaa
    22921 atgttttaca ggtctattcc gtcttaacga atttcttctc tatcaatccg ttatctgtat
    22981 agaccacaat ggaatacagg cctttggaca gcatggaggt gttgactgaa atctgtttat
    23041 tgttacccgt catctgatca atcatcacct gtccgagctg gttcaggata accatgcggc
    23101 agttggttgt tttacccgta aagctgatca tcatttcctc cttaacggga ttaggataga
    23161 tcatgaaaga agccattccg ttttctccaa cgccgataca ttcatcgatg accacttcat
    23221 attcgagggt ggtaaaagaa caaacgccat tggtttctgt gactttcacc cagccatcgc
    23281 cggcagcgcc ccaggtgacg gttatctcat ttgtcccgtt gccttcagta atggtcccgc
    23341 cgttgatctc ccatgtgtaa tcattaccat cggcaagttc tgtcgagtaa atgtcgccca
    23401 cctcataatc acaaaccagt tgcggcccac tgataacagg ctgcggagcg gcattgacca
    23461 ccacggtgag ggcatcggag aaatcgccgg taaagcagct attcacaccg gcaacgctga
    23521 tataagcatc tcctgaaaat tccgcgttcc agtcgaccgt ggctgtttca tcatttccac
    23581 tgatggagcc cgcctccgtt gggctgaggt tccaaaagta agatgtggcg ccatcaatat
    23641 catccgaaat atattctgtt cccgaatcgg aactgcaaac ctgtgtttcg cccgacgggg
    23701 tttcgccttc atccggcgga gtaatgataa atatataagc attgcctatc atatttttat
    23761 cacaataatc ggtgtatcca acgcaggtat aaatcccctg ttcggtctgg ttgccaaaag
    23821 tgatggccga acctgttccc gccataattt gtcctgtagg atcgccatcc agataaagct
    23881 cataatcgac accattctgc gacccgtcga gggtaagttc aatgccatcg ctgccttcgc
    23941 aataacccga accatcggat aaagtatatt cagatggagt catgtaagcc gcgcagttca
    24001 gtccctgcga ccaggtaccg ttgccacaat cgttatctgc cctgactttg atcgtgtagg
    24061 ttccaaggta tcccggggcg agggtgaaag tggctaccgg atcggggcct gagatggttc
    24121 ctgctgcatc gggttccaca tcccagacat acgttgtggc ccattgcacc agcggtgtcg
    24181 aatactgaat cccgtcattc tcctggcaaa ccgttgtcgg accgttaggc gtctggggct
    24241 ggaccggtgg ggtaatgacc aggatgtaat tgggattcaa cagtgtatca taaacggttc
    24301 cgtcataaac aataagctgg acatcatact ggccttgcgt attataagtt actaccgggt
    24361 tctgtgccgc ggacgttgcc gggtttcctg cttcaaaagt ccagttccag gaaatgattt
    24421 ctcccatcga ctgatcttca aattcgacgg ctgttgtgat acagggtgtc acgtcttcac
    24481 aggtaaatcc ggctgtggcc tgcattggct ccaggtcggg tatggctacc atatttccct
    24541 gcaaaacttc tttagtggct tcattctgaa cgaaggctac cagttcacag ttgctggcaa
    24601 cccagctggc atccattgta aacgaaagat ccagggtgac ttcgtcctgg ctggtgaaat
    24661 cgacaaccgt tcccatatgg gtcggggcca tgagcctttc cacaaagttc aggtgatcct
    24721 gcccctgcca ggaataaacg atttccgatt cggtcagcac cagatgtacg gtcagattgc
    24781 cccaggtgcc gtttgtttgc gaaatgacga gttgtatatt gtatgtattc cctgtatgtt
    24841 caccatagat atcaatgagg aaatcacttg gaatggccat ccgttgctgg tacagcggca
    24901 ggtagttagg atacatgctt tcggtatggc ttcctccgac atattcgaga accccgtcga
    24961 agtgggctgt cggatatcct gatacgttgt aatagctgtt cctgtaatcg gaagctgtat
    25021 tggcatacgg atcgccgtta tggtactcaa tgtttccaac ctggcacccg tataaaacga
    25081 gatcatcggc gcccatggct gcacccgggc agtaggtgca ccaggttccg gttccaattt
    25141 caacaataac tttttgcctt ccgacaggtt gtgcaaaaac agcaacaccc atcaggcaga
    25201 tcatgaaaag taagtagatt tttttcatcg gtaatgtttt ttgattcaga attttagggt
    25261 ctaaaaataa attattttca ggcatatata taaaatgcca gatatttata atctgtctaa
    25321 attagtgtgg gtaaaaccag gagggattca attcccctga agcaggtaaa cgatgtaacc
    25381 gatgtaaact gccagcatca aagccgcttc ccaacggcgt aaagtgaagc ctgtcttaat
    25441 aaaaggcagc agaagcaacg acatgccgat cattaccagg gtatcggtaa gattgaaccg
    25501 ggcgcttcga tgggctcaac catcgccgca atccccagaa tgctcagaat attgaagata
    25561 ttggagccga tcacgttgcc aatggcaata tcgggttgct tcctaaatgc tgcgacgatg
    25621 gaagtggcca gttcgggagt gctggtaccg acggcgaccc tttgacaagc ccgctgaccc
    25681 ccagatacaa tacagctaaa gaaatgatta tgaatagaat ataagtcaat atctgggtta
    25741 atattgaatt cttaattcat aattttaatt aaactttgaa ctttgatccg ccagcttgcg
    25801 gatgaacttt gaactaaaat atgtgtttca cgatctcccg gatattatcc gagttgccca
    25861 tggtgaaata gtgcagcgcc ggcacacccg cctgcatcag ctctttcgac tgcatggtgc
    25921 accattcgat ccccacctgc cggacctgtt tgctgtccct gcatttttcc acctctttct
    25981 gcaaatcttc cggaatggag atattgaaag tctttggaag cgtattcagc tgcccggcgg
    26041 tcgaaaccgg tttcaggccg ggaatgatgg gtaccgttat gcccgcttcc cggcattgtt
    26101 ttacaaattc gaaatagaac cggttttcaa aaaacatctg cgtgacgatg tattcaccgc
    26161 cggcatcgat cttttctttc aaatgcctga tatccgttct catgtttggc gcttcggcat
    26221 gcttttccgg gtatccggcc acaccgaccg aaaaccgggt cggggcgtta ttcaccagct
    26281 cgttgtcaag atatttgccc ttgttcaggt ccatgatctg gcggataagg tccacagcaa
    26341 attgattgcc ccctttttcc ggtttgaaga acttttcgcc cggaagatta tctcccctga
    26401 tcgccagcag gttataaatt tccaggaaat gaaggtcgat aaggccgttt tcggtctcct
    26461 ccctgttaaa tccgccgcaa ataatgtgcg gaacgacttc gatcttgtat ttgtacttta
    26521 ttgctgctga aatacccact gttccgggac gttttctcag gactcttttt tcaatacttc
    26581 catccggaaa ttccttgtat aatatttctt cccggtgata ggtaacctcg ataaaagcgg
    26641 gctcaaactc catcaacggg tcaatggcac gatagatcga attgatatcg cttcccttca
    26701 ccggcggaag gatttcaaag gtgaacaggg ggctccttga ctttgcaatt gcttcaacta
    26761 ctcgcatagg tattgatttt tctcacagat tttcacagat tcaagaggct gtctcaaaag
    26821 tccatatatt cacttttttt aggtagtccg acgactgaag ccgtcggttc taaccctcat
    26881 aaaacattcg gaaccaacgg ctttagtcgt tggagcacga actgttttat ggcaatcaaa
    26941 tcgaaatatc agacttttga gacatcctca tctgtatttt tctgtgttta tcagtgagaa
    27001 cttattattc atccattaaa ttttgtctta aatactttct ggccaggtct gaagagatat
    27061 tcttccgttc ggcataatcc cgtacctggt cgtccgtgat cttccccagg ttgaaatacc
    27121 tggaaaaatc atggctgaaa taatacccgc tgaccgatgc agcaggatac atggcgaaat
    27181 tctccgtcag gttgatcccc gcattttctt cagcctttaa aaggtcgaaa atggcccttt
    27241 tttccgagtg ttcggggcaa cccgggtacc ccggcgccgg ccgtatcccc cggtactctt
    27301 cctttaacat atcagaaacc ggaatgttct caccggcagc atatccccag aattctttcc
    27361 tgacgcgttc atgcaaccgt tcggcgaaag cctccgcaaa acggtctgcc agtatcttca
    27421 gcataaagct gctgtaatca tccagtgcat cttcgtaatg tttcacccat ttttctatcc
    27481 cgatgccggc cgtaacggca aaaagtccga tgtaatcagt ctttccgtaa ctttcgggtg
    27541 caataaaatc ggcaaagcta agattaggga cgccatcccg tttgggctcc tggtttctga
    27601 gaaaatgaag ctcggacaac gattctgtca tcttttcatc tttaaataaa accacatcgt
    27661 cgcccctgga aaccgccgga aataaaccga tgacaccgct ggctttcagc atccctttgt
    27721 ccaggatctc ttcgatcatt ttttgggcat ccgcgaataa tttccccgcc tcttcccctt
    27781 tcaccggatc gtcgaatatt tccgggtact tgccgggaat cttccaggcg tggaaaaaga
    27841 aggtccagtc gatataatcc ctcaattcag ccaatgggta atccccgaaa accgttattc
    27901 ctgtgaaggc aggcgcggtt atctcaatat tcttccaatc gacggataac cggttctttc
    27961 gggcctcatg aagcgggatc agcacatcct cattcttcct gttttcatgt tttatcttca
    28021 ggttacggta tttctcagca atctcctgcg tgtatgtttt tttattttcc ggagataaga
    28081 gtgatgacag cacaccgaca caccgggagg catccctgac atggatcacc ggatgatcgt
    28141 accggggcgc gatcttcacg gcagtatgaa tctcagaggt ggtggcgccg ccgatcagca
    28201 aagggatatc gaagttcagc cgctgcatct ccgatgccac atgcgccatc tcttcgagcg
    28261 aaggggtgat cagcccgctc aggccgatca cgtcgacatg ctgctcctgt gctgtctgca
    28321 ggattttctc ggcaggcacc atcaccccca ggtcgatcac ttcataatta ttgcacccca
    28381 gtactacacc cacgatgttt ttgccgatat cgtgcacatc gcccttgaca gtggcaagaa
    28441 gcactctggc ccgccccctc cccttggtcc cctcccccaa ttgggggagg ggaccaaggg
    28501 gagggggcgg gccagagtgc ttcttgccac tgtcaagggc gatgtgcacg atatcggcaa
    28561 aaacatcgtg ggtgtagtac tggggtgcaa taattatgaa gtgatcgacc tgggggtgat
    28621 ggtgcctgcc gagaaaatcc tgcagacagc acaggagcag catgtcgacg tgatcggcct
    28681 gagcgggctg atcacccctt cgctcgaaga gatggcgcat gtggcatcgg agatgcagcg
    28741 gctgaacttc gatatccctt tgctgatcgg cggcgccacc acctctgaga ttcatactgc
    28801 cgtgaagatc gcgccccggt acgatcatcc ggtgatccat gtcagggatg cctcccggtg
    28861 tgtcggtgtg ctgtcatcac tcttatctcc ggaaaataaa aaaacataca cgcaggagat
    28921 tgctgagaaa taccgtaacc tgaagataaa acatgaaaac aggaagaatg aggatgtgct
    28981 gatcccgctt catgaggccc gaaagaaccg gttatccgtc gattggaaga atattgagat
    29041 aaccgcgcct gccttcacag gaataacggt tttcggggat tacccattgg ctgaattgag
    29101 ggattatatc gactggacct tctttttcca cgcctggaag attcccggca agtacccgga
    29161 aatattcgac gatccggtga aaggggaaga ggcggggaaa ttattcgcgg atgcccaaaa
    29221 aatgatcgaa gagatcctgg acaaagggat gctgaaagcc agcggtgtca tcggtttatt
    29281 tccggcggtt tccaggggcg acgatgtggt tttatttaaa gatgaaaaga tgacagaatc
    29341 gttgtccgag cttcattttc tcagaaacca ggagcccaaa cgggatggcg tccctaatct
    29401 tagctttgcc gattttattg cacccgaaag ttacggaaag actgattaca tcggactttt
    29461 tgccgttacg gccggcatcg ggatagaaaa atgggtgaaa cattacgaag atgcactgga
    29521 tgattacagc agctttatgc tgaagatact ggcagaccgt tttgcggagg ctttcgccga
    29581 acggttgcat gaacgcgtca ggaaagaatt ctggggatat gctgccggtg agaacattcc
    29641 ggtttctgat atgttaaagg aagagtaccg ggggatacgg ccggcgccgg ggtacccggg
    29701 ttgccccgaa cactcggaaa aaagggccat tttcgacctt ttaaaggctg aagaaaatgc
    29761 ggggatcaac ctgacggaga atttcgccat gtatcctgct gcatcggtca gcgggtatta
    29821 tttcagccat gatttttcca ggtatttcaa cctggggaag atcacggacg accaggtacg
    29881 ggattatgcc gaacggaaga atatctcttc agacctggcc agaaagtatt taagacaaaa
    29941 tttaatggat gaataataag ttctcactga taaacacaga aaaatacaga tgaggatgtc
    30001 tcaaaagtct gatatttcga tttgattgcc ataaaacagt tcgtgctcca acgactaaag
    30061 ccgttggttc cgaatgtttt atgagggtta gaaccgacgg cttcagtcgt cggactacct
    30121 aaaaaaagtg aatatatgga cttttgagac agcctcttga atctgtgaaa atctgtgaga
    30181 aaaatcaata cctatgcgag tagttgaagc aattgcaaag tcaaggagcc ccctgttcac
    30241 ctttgaaatc cttccgccgg tgaagggaag cgatatcaat tcgatctatc gtgccattga
    30301 cccgttgatg gagtttgagc ccgcttttat cgaggttacc tatcaccggg aagaaatatt
    30361 atacaaggaa tttccggatg gaagtattga aaaaagagtc ctgagaaaac gtcccggaac
    30421 agtgggtatt tcagcagcaa taaagtacaa atacaagatc gaagtcgttc cgcacattat
    30481 ttgcggcgga tttaacaggg aggagaccga aaacggcctt atcgaccttc atttcctgga
    30541 aatttataac ctgctggcga tcaggggaga taatcttccg ggcgaaaagt tcttcaaacc
    30601 ggaaaaaggg ggcaatcaat ttgctgtgga ccttatccgc cagatcatgg acctgaacaa
    30661 gggcaaatat cttgacaacg agctggtgaa taacgccccg acccggtttt cggtcggtgt
    30721 ggccggatac ccggaaaagc atgccgaagc gccaaacatg agaacggata tcaggcattt
    30781 gaaagaaaag atcgatgccg gcggtgaata catcgtcacg cagatgtttt ttgaaaaccg
    30841 gttctatttc gaatttgtaa aacaatgccg ggaagcgggc ataacggtac ccatcattcc
    30901 cggcctgaaa ccggtttcga ccgccgggca gctgaatacg cttccaaaga ctttcaatat
    30961 ctccattccg gaagatttgc agaaagaggt ggaaaaatgc agggacagca aacaggtccg
    31021 gcaggtgggg atcgaatggt gcaccatgca gtcgaaagag ctgatgcagg cgggtgtgcc
    31081 ggcgctgcac tatttcacca tgggcaactc ggataatatc cgggagatcg tgaaacacat
    31141 attttagttc aaagttcatc cgcaagctgg cggatcaaag ttcaaagttt aattaaaatt
    31201 atgaattaag aattcaatat taacccagat attgacttat attctattca taatcatttc
    31261 tttagctgta ttgtatctgg gggtcagcgg gcttgtcaaa gggtcgccgt cggtaccagc
    31321 actcccgaac tggccacttc catcgtcgca gcatttagga agcaacccga tattgccatt
    31381 ggcaacgtga tcggctccaa tatcttcaat attctgagca ttctggggat tgcggcgatg
    31441 gttgagccca tcgaagcgcc cggttcaatc ttaccgatac cctggtaatg atcggcatgt
    31501 cgttgcttct gctgcctttt attaagacag gcttcacttt acgccgttgg gaagcggctt
    31561 tgatgctggc agtttacatc ggttacatcg tttacctgct tcaggggaat tgaatccctc
    31621 ctggttttac ccacactaat ttagacagat tataaatatc tggcatttta tatatatgcc
    31681 tgaaaataat ttatttttag accctaaaat tctgaatcaa aaaacattac cgatgaaaaa
    31741 aatctactta cttttcatga tctgcctgat gggtgttgct gtttttgcac aacctgtcgg
    31801 aaggcaaaaa gttattgttg aaattggaac cggaacctgg tgcacctact gcccgggtgc
    31861 agccatgggc gccgatgatc tcgttttata cgggtgccag gttggaaaca ttgagtacca
    31921 taacggcgat ccgtatgcca atacagcttc cgattacagg aacagctatt acaacgtatc
    31981 aggatatccg acagcccact tcgacggggt tctcgaatat gtcggaggaa gccataccga
    32041 aagcatgtat cctaactacc tgccgctgta ccagcaacgg atggccattc caagtgattt
    32101 cctcattgat atctatggtg aacatacagg gaatacatac aatatacaac tcgtcatttc
    32161 gcaaacaaac ggcacctggg gcaatctgac cgtacatctg gtgctgaccg aatcggaaat
    32221 cgtttattcc tggcaggggc aggatcacct gaactttgtg gaaaggctca tggccccgac
    32281 ccatatggga acggttgtcg atttcaccag ccaggacgaa gtcaccctgg atctttcgtt
    32341 tacaatggat gccagctggg ttgccagcaa ctgtgaactg gtagccttcg ttcagaatga
    32401 agccactaaa gaagttttgc agggaaatat ggtagccata cccgacctgg agccaatgca
    32461 ggccacagcc ggatttacct gtgaagacgt gacaccctgt atcacaacag ccgtcgaatt
    32521 tgaagatcag tcgatgggag aaatcatttc ctggaactgg acttttgaag caggaaaccc
    32581 ggcaacgtcc gcggcacaga acccggtagt aacttataat acgcaaggcc agtatgatgt
    32641 ccagcttatt gtttatgacg gaaccgttta tgatacactg ttgaatccca attacatcct
    32701 ggtcattacc ccaccggtcc agccccagac gcctaacggt ccgacaacgg tttgccagga
    32761 gaatgacggg attcagtatt cgacaccgct ggtgcaatgg gccacaacgt atgtctggga
    32821 tgtggaaccc gatgcagcag gaaccatctc aggccccgat ccggtagcca ctttcaccct
    32881 cgccccggga taccttggaa cctacacgat caaagtcagg gcagataacg attgtggcaa
    32941 cggtacctgg tcgcagggac tgaactgcgc ggcttacatg actccatctg aatatacttt
    33001 atccgatggt tcgggttatt gcgaaggcag cgatggcatt gaacttaccc tcgacgggtc
    33061 gcagaatggt gtcgattatg agctttatct ggatggcgat cctacaggac aaattatggc
    33121 gggaacaggt tcggccatca cttttggcaa ccagaccgaa caggggattt atacctgcgt
    33181 tggatacacc gattattgtg ataaaaatat gataggcaat gcttatatat ttatcattac
    33241 tccgccggat gaaggcgaaa ccccgtcggg cgaaacacag gtttgcagtt ccgattcggg
    33301 aacagaatat atttcggatg atattgatgg cgccacatct tacttttgga acctcagccc
    33361 aacggaggcg ggctccatca gtggaaatga tgaaacagcc acggtcgact ggaacgcgga
    33421 attttcagga gatgcttata tcagcgttgc cggtgtgaat agctgcttta ccggcgattt
    33481 ctccgatgcc ctcaccgtgg tggtcaatgc cgctccgcag cctgttatca gtgggccgca
    33541 actggtttgt gattatgagg tgggcgacat ttactcgaca gaacttgccg atggtaatga
    33601 ttacacatgg gagatcaacg gcgggaccat tactgaaggc aacgggacaa atgagataac
    33661 cgtcacctgg ggcgctgccg gcgatggctg ggtgaaagtc acagaaacca atggcgtttg
    33721 ttcttttacc accctcgaat atgaagtggt catcgatgaa tgtatcggcg ttggagaaaa
    33781 cggaatggct tctttcatga tctatcctaa tcccgttaag gaggaaatga tgatcagctt
    33841 tacgggtaaa acaaccaact gccgcatggt tatcctgaac cagctcggac aggtgatgat
    33901 tgatcagatg acgggtaaca ataaacagat ttcagtcaac acctccatgc tgtccaaagg
    33961 cctgtattcc attgtggtct atacagataa cggattgata gagaagaaat tcgttaagac
    34021 ggaatagacc tgtaaaacat ttttgagtaa gatccctcac tgtgttcgtg acgcatcgtc
    34081 atcccgaacg cagtgaggga tctctgtttt agaagatcct gtaccgggca atcctgtgct
    34141 tatgcaggaa gtaccggaat gaaaccccaa gtacgcccag cccgtatttc atgcttcttt
    34201 tcaggttgat ggaagaggcg tcgtcgaaat atttggtcgg gcaggtgact tcggcaatct
    34261 catacccggc aaagaagatt tgggccagca tctggttatc gaagataaaa tcgtcggagt
    34321 tatgctgata tttgatattc tgaagaacct ccttcgaaaa agccctgtag ccggtatggt
    34381 attccgatag cttttgcttc ataagcatgt tctgtgccag cgtaaggaat cggttggata
    34441 tgtacttgta aagcggcatc ccgcccctga gagcaccttt cccgagaata cgcgatccca
    34501 ggacaacggg gtaaacgtcg ttagcaatga cgtagcacat gctgtggata agctttggcg
    34561 tgtactggta atcgggatga agcattacga cgatgtcgga accgatctcg agcgctttat
    34621 cgtagcagct cttctggttt cctccgtatc ctttgttttc ctggtgctcg atgatatgat
    34681 ggatcccgag ccgttttgcc acctccacgg tgttatcccg gcttttatca tcgaccagga
    34741 tcacctcatc cactatatcg aacggtatct cccggtaggt ttgttcaagg gttttttccg
    34801 cattgtatgc cggtagtacg acagttatct tcttgccata aatcatattc ctgcatttta
    34861 ttattcagga tatcgcgccg caaagataaa accgttcttc tcgtataaaa cggtcagttg
    34921 aggataaaga cgggaatatt cttctttctt cgttatttta tatacaaaat aggcgttctt
    34981 atcaatcttt ccgttgagca gccattcttt atcataagaa atcggattga ccggcggcat
    35041 tttctttcca taaaaaaggt gggcataact tttaaaaccg agggtattga tataacactc
    35101 ctcactactg tgtttttcat aaaattcgat gggagcacgc tgaacatatc cttctatttt
    35161 accggtgaaa atggtcatgg tgaggaaggt aaagaccagg gaggtgctga aaagcgcaat
    35221 cattttatga aaaggttttt tcaggatgaa gaagccggca attcccggga tcagcaccag
    35281 ggcaaccaga gtctcgaagc ctgtccaccg gatgtcggcc gccaggttgg cgacggcaaa
    35341 ggggtctttg atcaggccac tgttcatcag ccacactttg ttcagaccaa ccagtgtaaa
    35401 tcctgcgatt accgtcatcc agaggatggc cagggtccac aggatgatgc gggaactgcg
    35461 ggaaaatgct ctctctgaaa ggctggcccg gtaaagggcc agggcagcca ggtaggtcag
    35521 cgggaaatag gccagggaac tgtaatgaac gatctttgtc tgaacaatct cgaaaagaac
    35581 caccacggtt gcgagaagga tgatcatcac ccggcgccag tttttttgca ggaaattctc
    35641 ttccgggacg gcctttagtc ctccgaaaaa gaatgccgat gccgggaaga cacccagcat
    35701 caggataacc acgtggtatc ccgggaaacc gccgtggcct gcgtctttgg tgctgaacag
    35761 gcgaacctgg tatacgatga agtcgatcac cacatcgagg tggccgttgg ctatcatgag
    35821 aaggaaccag gagccgccgg tgaggaggag accgatggcg aaaagagaga gggattttgt
    35881 gataatttgg aaatttggaa atttggagat ttgattctgt gaagttgtgg attttttgat
    35941 gttggtcagg atgaaagaga acgggaagac tatggcaacc agtccgaaga tcagaagggc
    36001 gacggggcct ttggtgagga tagccagacc aatggcggca ccggaaagaa agacaaggag
    36061 accaggggaa tgtaggaatt tatgactgtt ggactgtaag acggtaggat tgtaagacga
    36121 gatgtcgggt tcattaacaa tcgaaccatt gaacaattga actcttgatc tgtcgatgga
    36181 aatggttgca aaatatatgc ccaggaaaat aaacaggttg aaccagggat cgatgatgcc
    36241 cgatttgaaa tagaagaacg gaaggatgga accggcgtaa accaacaccc aggtgaggcc
    36301 cattttttca ttatggattt tttttccgat ggcgaagatg accagcagcg ttgtgatgcc
    36361 gcaaatagca ttggggaaac gggcggcgaa ttcattgatg ccaaaaagct tcatcgacaa
    36421 cgcctgcatc cagatgaaca gcggcggctt ttcccagaat ggctgataat tgatctgcac
    36481 gttgaggtaa tcgcccgtaa cgatcatctc ccgggcgatc tcggcaaaat tgacctcatc
    36541 ccagtcgaac agggcgacgt tgcccaggaa aggcaggaaa aggagcgccc cggcgactac
    36601 tatcagtaag tactgaaggt tgttgttgta tttctgaccg atcaagattt caattatttg
    36661 aatgtggtaa aaataataaa tgatttacag tttacggatt acagtttaca atctataatc
    36721 tgaattttgc attgaagatt gtactctgca gaatgtaaaa agtaatctct aaattgtaat
    36781 tcttgacaat tgtaactacc acaagattaa tgaatagaaa aattttttta actaaaccgg
    36841 gtatttattg tgagaaaatg ggattaatta gaaaaaacaa tatgaaccgg attgaactgg
    36901 aaaagcgact aattagcttt gcatcggcaa ttattacgct ttgtaaggaa tgtcgttttg
    36961 atgattatac ttttcatctt gctaaacaga ttgtaagatc tgccagctcg gctgcattaa
    37021 attatggtga agcacagagt gcggaatcac gtgcggattt tactcataaa cttagccttg
    37081 tactcaaaga actgagagaa actcaagtga atataacaat aatgagtaac tctgtaattt
    37141 gtaataatcc tcttagaata gtagatcttt taaaagaatg tcaggatctc attgctattt
    37201 ttcaaaaatc cattaacaca ctgaataata agaataaccg atgatcatat tccataatcc
    37261 acttcaaaga ttgtaaactg taaattgtaa atcgtaaatc gaagttcctc actcgtactc
    37321 tccccacgcc ttgatcccga tctcctcgat cccgatcctg cagaacgagt agataaatgc
    37381 gctggccagc cgggcattgg tgatgagcgg gatgttgaaa tcgacggcac tgcggcggat
    37441 gcggtaatcg ttgtcaagct cgcttttgct caggtccttg gggatgttta tcaccaggtc
    37501 gatcttcttc cggcgcaggt attccatcgt gttcggggtt ttatcctcgc ccggccagtg
    37561 cagcatgatc gaattaatcc cgtttttgat gaagaaatcg tgggtgccgc cggtggcata
    37621 aatggtcaga cctttctcct gcagcatccg ggcgctggtg agcaattcct gcttcgaccg
    37681 cgacgggccg ctggaataca gcacagcttt ctccgggatc gtatagccga ccgacaacat
    37741 cgatttcaga atggcttcgt agtaattgtc gccgatgcag ccgacctcgc cggtggaaac
    37801 catttcgacg cccagcacag gatcggcttt ttgcaatcgg gagaatgaga actgcgaggc
    37861 tttgacgccg acataatcga gatcgaacaa cgatttatcc ggtttggcca ccgtttttcc
    37921 cagcatgatt ttcgttgcgg tttcgataaa attggttttc agtacttttg agatgaacgg
    37981 gaagctgcga gaggctctga gattgcactc gatcaccatc acatcgttgt ctttggccag
    38041 gaactggatg ttgaacggcc ctgaaatttt caggtgttgc gcgatctgcc ggctgatccg
    38101 cttgatccgc ctcaccgttt cgacatagat tttctgtggc ggaaacagca tggtggcatc
    38161 tcccgagtga acgccggcga actcaacatg ttccgagatc gcatagcaaa tgatctcccc
    38221 gttcgcggct acggcatcca tctcgatctc ctttgcgtct tcaatgaacc tggaaaccac
    38281 cacgggatac tgtttcgaca cctccgccgc catctccagg aaatgttcga gctcgctgcg
    38341 gttcgacaca atgttcatgg ccgcacccga caaaacatac gacggcctta caatgaccgg
    38401 gaaaccgacc tcatcggtaa accggtatac ctcgcttacg gaagatgact cgatccaccg
    38461 gggctggtcg atgccaagtt cgtcaaggat agccgaaaat ttgtgccggt tctcagcttt
    38521 gtcgatcgac tgcggcgagg tgccgagaat cgccacctcc tgctcccaga gccgaatcgc
    38581 caggttgttc ggaatttgcc cgccggttga aacgatcacg cccagggggt tttcaagatc
    38641 gaggatgtcc atcacccttt caaaggtaag ttcctcgaaa tagagccggt cgcacaggtc
    38701 ataatcggtg ctgacagtct cggggttgta gttgatcatc accgaacggt atccttcctt
    38761 attaacacat tccagcgcat tcaccccgca ccagtcgaat tccacgctgc tgccgatccg
    38821 gtaggcgcct gaaccaagga cgacaacggt ttctggtgtt tcctcttttt gtagtccgac
    38881 gactgaagcc gtcggttcac gctgttcttt tcttccgacg actaaagccg tcggttccaa
    38941 atttaatcca tcctcggaac caacggcttt agtcgttgga gtagtaacat tctttgcgga
    39001 aaagccaata tcgtgctcgt tgccattgta tgttaaatag agataattgg tccgggccgg
    39061 gtattctgct gccatggtgt caatttgctt cacataaggc aggatgcctt ttgctttgcg
    39121 ataatttcgc actttcagca ggtcatccgc aatgtgatcc cggtcacttt taaaaatgaa
    39181 cctggcgatc tgaaaatcgg agaagccgag ctgttttgat ctcagcaaaa gggcgagggg
    39241 cagttcttcc agggtgttgt aggttatcac ttcattgcga atgtcccaga tgtttttcag
    39301 tttttccagg aaccagcggt cgatcttcgt cagttcgaaa atgcgttcca cgctgtagcc
    39361 tttcatcagc gccatcgcaa tgacaaagat gcgcatatcg gtgggcacgg ccagctcttt
    39421 gtcgatgatg atatcctcga tgtctttgtt gccgacaaac ccgtgcatcc cctggccaat
    39481 catcctgagc cctttctgga tggcttcttc gaaggtgcgc ccgatggcca tgacttcccc
    39541 cacactcttc atgctgctgc cgatttcgcg cgaaacgcca tggaactttc ccaggtccca
    39601 tcgggggatt ttcacgacga tatagtcgag ggccggttcg aaaaatgccg atgtatcctg
    39661 tgtcaccgaa ttcttcagct caaacaggcc gtagcccagg gcaattttgg ctgccacaaa
    39721 ggccagcggg tatcctgtgg cttttgacgc cagggcgctc gaccgggaaa ggcgggcatt
    39781 gacttcgatc acgcggtagt cttcggagtt gggatcaagg gcgtactgca cgttgcattc
    39841 accggtaatt ccgatatgcc tgacgatccg gatcgatagt tcccggagct tgtggtattc
    39901 cctgttgctc agggtttgcg aaggtgctac tacaatgctt tcacccgtgt ggatccccag
    39961 cggatcgaaa ttctccatat tgcacaccgt gatgcaattg tcgaaaccgt cgcgtaccac
    40021 ctcgtattcc acctctttcc atcctttcag cgattcttcg accagcacct ggctggaata
    40081 ggagaaggat ttctgtgtca gcgccctcat ttcgtcttcc gatccacaga agccgctgcc
    40141 cagtccgccg agggcaaaag ccgagcggac gatcacggga taacccaggg ttgccgccgc
    40201 ctgcaccgcc tcatccacac cggtcaccgc gatgctgcgc ggtgttttta catggatctg
    40261 atccagtttc cgggtaaaaa gttccctgtc ttccgtgtcg aggatggcgc tgacgggtgt
    40321 tcccaatacc ctgaccccgt gtttctccag tatccccgac tggtgcaagg cgacgccgca
    40381 gttcagggcg gtctgcccgc cgaacgacag gaggatgcca tccggattct ctttttcaat
    40441 gactttttcg acgaagaaag gggttaccgg gaggaaatag atcttatcgg ccatcccttc
    40501 ggaagtctgg accgtggcga tgttgggatt gatcaggacg gtagcaatac cttcttcgcg
    40561 aagggctttg agcgcctggg tgccggaata gtcgaattcg ccggcttcgc cgatcttcag
    40621 ggcacctgat cccagcagga ggactttttt aatggcgtgc ttcatattcc ctgacttttt
    40681 ccataaaatg atcgaacagg aattcggtat cgaccggacc ggcagaggct tccgggtgga
    40741 actgtacggc gaagaaaggt tttgagctgt ggatgatgcc ttcgcaggta ccgtcgttca
    40801 gattctcgaa ccagggtttc caaccctccg ggagtgtctc tgtatcaaca gcatagccgt
    40861 ggttctgaga agttatgaag caccggtggc tctctttcat gattacgggc tggttgtggc
    40921 tccggtgacc gtatttcaac ttgtaggtat cggcgcctgc ggccagggcc atgatctgtg
    40981 agccgaggca gatgccaaag accggcagat cgccatcaag gaggttcttc accgattcta
    41041 tggtggcttt gcacatctta ggatcacccg gaccattgga gaccagaatg ccgtcgtatt
    41101 ccgcgggatc cgccgggtgg tcccagggga cccggtgcac agtggcatct cttttcagca
    41161 ggcaccggat gatgttgttt ttagtgccgc aatccaccag gaggatacga cgacggccat
    41221 tcccgtaggt tattttttcc ctggccgaaa cctggttgac aaggttgtct ttgttcgggt
    41281 cgtaaaaatg tacatcatcc tgcactacga ttttccccag catggcccct ttttcccgga
    41341 tgatccgggt cagtttccgt gtatccacac cggcaatgcc gggcacccgg tgttctttca
    41401 gccagtccga caggcttttt actgcattcc agtgactgta actgaaagag tagtcggaaa
    41461 tgaccaggcc ggagatctga actttatccg attcgaagaa ttccgtcatg ccttctttca
    41521 tccgcatttc aggcaccccg taattgccga taagcgggta ggtggctacg aggatctgtc
    41581 ctttgtacga cgggtcggtg aggctttccg ggtagccgtt cattgcggtg gagaagacca
    41641 cctcgccgga tgaagattgt tcggcgccga aggaatagcc gcggaattcc gtgccatcat
    41701 ccaggataag cgttgcagga accgttttat tcatagacca ggcagttgat attcaattat
    41761 atgatttccc ggatctaaaa atatatgttt tgataaagat atttcggatt gttgaaaagt
    41821 aattgagatg gcatctctga catcatgaaa atgcttccta acaaacacat aataaattat
    41881 ttaaagaaga aatctattga tttgagatgc catctcataa aatggtgctt attatttcta
    41941 tttaaaagaa aatcaacctt ctgaactcca agtttattat ccttattact cagtaaaatc
    42001 caagactagt cttccagttt aaaaccatta atttttttat cattctttat aatcagatga
    42061 tcttcaataa gattggagta tcctccaaac caattaatta attccgcaga ctcaaaatca
    42121 ttgatttgat tgcggagaat tttgtaaaaa gtactgtatc tataagtata aatatcgtta
    42181 gtaatcttat gctttatcgg atttaaatgg atgtatcgca ctaggctaat aaagtattcg
    42241 tcgcgattta cctcaattcg cctgaagttt ttactaataa tacagccagt tctgcattct
    42301 tgacgattaa tatacattgc ataagttata aacagcttcc ttagttgatt tgaaacatac
    42361 cttgaatcgg ttgctgaatc ctttatacga attaaaaaat gaaaatgatt tggtaacaga
    42421 caatagcaaa atgtttcagc aaccggatcc atatagtaga agtacttttt aataaaaaag
    42481 tcataattgc aatcgaaata gaaaagtcta tctctattat ttcccctgtt gaataaatga
    42541 taatacttcc ccgcaattag aggtgtcttt aaagattgct ttttcattgt aaattgatta
    42601 taggtttaac aaaaggatga attctcaatt gttaatccgt aatttgacta caaaataccc
    42661 ggacccttaa aaatattttt tagatggcat caggattaca agttcctgga agcgatatgt
    42721 gattataaat aagcagttta gaatatgaaa tctaattctt tgagatggca tctcaaaatc
    42781 aaataagatc cggtcttttc acccgcaaat cagcttatat agaaaatttc ttttgcatat
    42841 tgagatgcca tctcgaagaa acacctcaat gaaaactata tgctcgccct tcttcaagta
    42901 tcaggaatct ctcatctaaa attccctctc tgctttttgc ttccttaagc tctttcgccg
    42961 gatctgatat cccgtcgtcg gccagcggga aggtgccgaa atgcatcccg atgctttgag
    43021 gggattttag gatattgtgg atattcaccg catcggccgg tgatgtgtgg atcggcgaca
    43081 tgaaccactg cggtttatag gccccgattg gaaggaaaga aagccggatc ggagagagcc
    43141 gttcggctat ttcgttgaaa aactctccat agccggtgtc tcccgaataa tatatgctcc
    43201 cgttttcggt gcgtaatgca aatcctgacc agagcgtcct gtccctgtcg aacatccccc
    43261 ggccgctgaa atgctgcgcc ggcgtacaaa ccagcttcaa cccgtttggc gttgtcaatt
    43321 cgtcccacca atccatttcc ctgaaattgt caatgccctt tttctttaag taaaggccca
    43381 ccccgagcgg cgtgtatatc accggtttaa aacggcttgt caattttttt aaggtactga
    43441 tatccaaatg gtcgtagtga ttatgggtca ataaaataat gtctatagga ggaagttctt
    43501 caaaccggat tcccggctgg cgcatccgct tcgggccggc aaacgtcaca gggctgaccc
    43561 gttcgctcca gaccggatcg gtcaggatat tcaacccctg cgtctggatc aggaaggtgg
    43621 aatggttgac gaaagtcaca acaatagaat caccttcgac tctttcttcc ggggcagggc
    43681 cataattcac ttcggtgatc tctttccatt caccccgttg cctttccctc gaccatttga
    43741 tgacatcttt cagccctccc gctttctttc cacccggatt gatgaatctc ttcccgtcga
    43801 aatggtcgct gacaggaccg tggtaaacag gcgccgaaaa caaccatccg agtaaaaggg
    43861 aacccagaat aatgatcaga attacaatga gaatgatgaa caatatcttc atagtttggt
    43921 tttaatcgaa tgcaaaagta taaactgttt aatctggtct aaacaatccg ctgatacatc
    43981 ggttcattac ggaatcaact tttatcagga atatattatc ttcgctttaa aatctctacg
    44041 gtgatcaaac atatcttatt catcatcatt gttttactgg caaataattc cttctgccag
    44101 cctgtttata aaacaaagaa tggcacggct tcctttgtat ccaatgcccc gctggagatc
    44161 atcagggccg aatctgcaga ggtgcagggc gccatcgatc cggggaagaa aacattcgcc
    44221 ttttccattg ataataagag ttttaaaggg tttaacagcc cgctgcaaca ggagcatttt
    44281 tatgaaaatt acctggaggt tcagtctttt cctgcctcaa cttttaaagg gaagatcatt
    44341 gaagatatcg acctgaactc ttcactggag caaacggtca gggccaaagg aatgctgaat
    44401 attcacggcg ttgaacagga aaggattatt aaaggaacga tccgggtggt agatgggaag
    44461 atcagcctga aggcagattt tagcgtgatg ctggaagacc atcagataag gataccccgg
    44521 gtcgttaacc agaagattgc ggaaagtatt gacgtttcag tcactgccga actggtaaaa
    44581 agcaatgatt aatggtcaga aaataaggag attgtggagt cctttgtttt ggctcacggc
    44641 ttttatcttc ctgattgccc attcaggatt ctcacaactt ccgggctttg agaagatcag
    44701 gatcaccgtc ccgggggaag gacctgtctt taaaaaagct gttatggatg cagatggata
    44761 catctggctg ggttccggac agggcctgtt ccgtttcgac ggcattcagt tcaaatcttt
    44821 taccccctta ccggatacgg ctgatttcca cgtgactgca cttcaccttg accaggatca
    44881 cgatctctgg attgggtgca gggacggtaa aatataccgg ttggatcagg gccggatcaa
    44941 cctctttaat ccggaagagg gtacttccgg tgtggctgtc accgatatcc tggtagccca
    45001 agaccgtagt atctggtggt cgacagccgg cgaagggatc tatttttact ccggcacccg
    45061 ggtcttcaat ataaaccgtg acgacggcct tcccgacgat tatgtgcatg accttgaatc
    45121 tgtgagcgat ggatatgttg tggcgggtac cgatggcggg ctggtttcgt gtatttacag
    45181 aaacggggag aaaatagtca atccgcttgc ggcgtccggt gaattgccgg atataatggt
    45241 cactgtggtc aaagaggata gtgatggcaa cctctggctg ggtttccagg acggagggat
    45301 tggttttttg acgaacgacg ggcggcaatt tattcagcct gcggcagatc atgcctggga
    45361 agagggtccg gtgagcgcag tgacggtttc cggtgcatcg gcgtgggcag gcacttcaag
    45421 cggtgaatta ctggagatca gtctgaatga gaataatcgg ggaatgatta atctgttacc
    45481 cgctgacctt cagaccggca aaatttatga cttgctggaa gaccgggaag gcaatatctg
    45541 gatactttct gccgaagggc tgttccggtc gaccgggaac aggattcgat acctggatga
    45601 tgttggcggg attccgctgg cgaatatcca tgccatccat gccgacgagt tccgtcccgg
    45661 cctgctgtgg ttcagcgacg accgcgggtt gttctccctg gccgtcgaat cgggcgatgt
    45721 aaaatcttac ccgggtccgt ttaaagggcc gggactgaaa atcacctgcc ttaaccagga
    45781 ccgctttggc tacatctggg caggtacttt taactacggg gcgatcaggc tcaatcctgc
    45841 aaatggcaca tttgatcgaa ttaccgaaag ggataacctg gtcaataaca atgttttatc
    45901 catatcccgg catcacgaca ccttgtggat ggcaaccctg ggaggagctt cggaaatcat
    45961 tcttgacccg aatacttccg gaagatctta tcaggcccgg tcatacaatt atgaaaacgg
    46021 gctggtcaat aatttcatct attatgttta tgaggatgat aaaaacagga tctggctggc
    46081 gaccgacgga gacgggatca atgtcaggac gcaggacgga tggtcttcat acaatgaaaa
    46141 caatggcctt accgatgatg tgatctattc ccttacgggg gataaatatg gcaatatctg
    46201 ggccggatcg gcatcgggcg gattgtttaa attcgatggg aagaacttca ttcactatgg
    46261 gcttgacgag ggtctgagca gcatttcctt caccgggatc atggcagcag acgatgaact
    46321 tctggccgtt catgagaatg gcctggatat cctacacatt ccaacgggaa acatcgctca
    46381 ttatggcggt gacaaaggat ttgaagggat ccagcccgat ctcaacacga tcagccgcga
    46441 ccgcgaaggg aatatatggc tggggacacg gaaagggatc atccggtacc ggccggggtc
    46501 ggcggtttct tatatgcccg tcacggttct ggaggagatg tcggtttacc tggaaccccg
    46561 ggagttgaag gccggccagg tgttcggtta tgccgacaac cacatttcat tccggtatgc
    46621 aggcctttgg ttttccaatc cggaaagagt gaattaccag gttatgctcg aagggtacga
    46681 cctgggctgg aacgatactt acaaccgcca ggtgacctat tccagccttc cgcccggtga
    46741 atttaccttt aaagtaagat cctcgctcga ccggtctttt gtcaatgccg gcgaagtcag
    46801 ttacagcttc aggataaggc aacctgtctg gatacaaccc tggtttatca tcctgctggt
    46861 cattatcctg gcggcgctta tctttctata cattaaaatg cgtgaaaaca ggctcagaaa
    46921 gagagaacag gagaaaaaag agaagatcga gtttgagttc caggtgctga aaaaccaggt
    46981 caatccacat tttctcttca acagtttcag taccctgatc tccctcatcg aagataatcc
    47041 tgaacaggcg gtacaatata cggaaaaact atctgatttt ttcaggacga tcctgcaata
    47101 caaagaccag gagctgatca ccctggatga agaacttaca ctcatacaaa gttatttctt
    47161 ccttttgaag aaacgttatg gagataacct ttacctggat attcagcttg gtgatgagct
    47221 caaaaaggca tttatcccgc ccatgacgct tcaaatcttg atcgaaaatg ctgtgaagca
    47281 taatgttata tcgatgagta agcctttgtt tattaaagtt tacgaagatg atgggaagat
    47341 cgttgtagag aataatctcc agcccaaact cactcccgaa gtctccaccg gcatcggact
    47401 tgaaaatatc aggaaaagat ataaactgat taccgataaa gaggtcataa ttgagatatc
    47461 ggaactgctt ttcaggattt ttttaccctt gatcaaatga ttattatact acaaagatcc
    47521 acgaaggcct tagtccggca gtcaatcact ttgtgctcct ttgtgaaacc tttgtgtact
    47581 ttgtggttaa ttaaatgaaa taaagaaaat gaaacttctt attattgaag atgaaccggc
    47641 aagcgcccag cggctgaaaa agatggtgga agagatggat cccgaggtga aggtcctcgg
    47701 cattctcgac agcatcacct ccaccatgga ttggttcggc catcaccccg agcccgatct
    47761 gattctatcg gacatccacc tggccgacgg gctcagtttc gaaatcttca ggcagatcaa
    47821 catttcctgc cccgttatct tcaccacggc atacgaccag tacgccatcc aggccttcaa
    47881 gctgaacagc atcgattacc tgctgaagcc cgtcaggaag gaagaactgg ctgaggcgat
    47941 caataaattc aaaaaggtga agccggcgga aaagcaggtc gatttatcgc agctcatggc
    48001 gctgataggc aaacaggaga aagaatacct caggcgggtg gtgatccggt tggggcagca
    48061 gatcaagatc gtggagatca aagatgtggc ttatttttat atagatgaaa agatcgtttt
    48121 cggcgttacc ttcggcaaag accgctaccc gatggacctg agcctcgatc agctggagaa
    48181 gcaactcgat cccgggcggt tcttccgaat caaccgcgcc tttatcatca gcctcgattc
    48241 gatcgaaacc atgatcacct actccaaagc ccgcatcaag atcaagctga aaccgccctg
    48301 cgagctggag tctatcacca gcaccgagcg gtcggccgag ttcagggagt ggctgcgggg
    48361 aggttagtcc atctgcccaa atccctcgct ctactcggga caggctgtcc aactgcccaa
    48421 cttccccatt caagtctcac tttttcccgt tcatccaata ttatttggaa ctcgccctcg
    48481 gttcgacgtt actttgcctc atcaaacaaa aacaccatga agactacagc aaaacttttc
    48541 atcctcgcac tgaccatcag cctggcttcc tgctccaaga gcaacgacga taatccttcc
    48601 aataccaata acaccggaca ggtgaccggt aactggaaag taacctacta tttcgacagc
    48661 ggcaaagacg agaccagcga ttacaacggg tacactttta atttcaatac cgggaatatc
    48721 ctggaagctg ttacgggctc cgcaacttat accggcacct ggaccttagg cgaaccgggc
    48781 gacgacgata actccagtaa caaatttgtc attaccatta caggaaacaa agccatggat
    48841 gacctgaccg acgactggaa aatacttaaa atgaccgata cggagatctg gctgcaggac
    48901 gataacctga cgagcgcgga agaactgaga ttcggaaagc tataagtgtt ccagtgttcc
    48961 aaagttccag tgttccaatg ttccaggttc cagagttaat agtgttagta aatagagatt
    49021 tatagcgctt ttgtgcaaaa aataaagaaa ccatgaaaaa aagaacaatc ataaccatca
    49081 ttctgtccct gtttgtcata ggggtgatca gcgcaggcct tgtttataaa ttcgtcatca
    49141 acaagcctca cgaaaactat gagaaagcca aaccggcctt tattatctct gccaccgacc
    49201 tgtataacag ctttcagtcg gaccggacga cagccgaagg taaattcaac ggcaaagtgg
    49261 tgctgctgag tggtaaactc gataagatcg atttagctga aagccttgtt accggggtat
    49321 ttgtctttaa ccaggggatg ttcggcgacg aagggatccg ctgtgttatg ctcggcaacc
    49381 atgcccaggc gctgaaagcc atcggaccgg gaacagaagt aaagatcaaa ggatacctga
    49441 ccggctacaa cgataccgat gtcgtcctgg agcagtgttc catcatacgt taaaaattca
    49501 atcgattatt aattcaaaag aatcaaataa aatgaaaaag tcacttaaaa tccttgtcat
    49561 cgcggctata gccataaccg catcgttcac cgtccaggcc cagaaataca tgaccaagaa
    49621 cgggaatatc aaattctact ccgaaacccc catcgaaact atcgaggcta ctaacaacca
    49681 ggtaaatgct gccctcgaca gccaaaccgg cgacctggta ttcaaagttc ttatcaagtc
    49741 gttccagttc gaaaaagcac tcatgcagga gcatttcaac gagaactata tggagagcga
    49801 caagttcccg aattcgacct ttgtcggaaa agtgaccaat cttactgctg ttgatttctc
    49861 aaaagaaggg tcttatgaag cgacgatcga gggcgacctg accattcacg gcgtaacgaa
    49921 gaagatcagc gaaaaaggga cgtttaccgt caaggccggg gataagatcc acgggatcag
    49981 caaattcaac gtgaagcctg ccgattattc gatcaaaatc ccgggtgcgg ttgtcaaaaa
    50041 cattgccgaa accattgaag taacggtaga tatcgagctg tcgaaaatgt agcagcacac
    50101 cactgttccc catatatctc ctccccggat cgcttttaac agggctttcc ggggtttttt
    50161 atgctgagat gactcaaaaa gttttaacca ccaaggccac aaaggaatca caaaggccac
    50221 aaagtaattg attaccaaat taaacccttt gtgttacttc gtgaagtcct ttgtgtctct
    50281 ttgtggttaa ttaataaatt tgcctgcaat aatcgtaaag cgttgatgaa caaactgaag
    50341 aaaatctggg ccgaggaata cccgatctac tggtacgaca ccgactgcaa cgggcaattg
    50401 aaaatgtcgg ccgttgccaa ttacctgcag gaatcggcat ggaggcatgc caaccatctc
    50461 ggctttggct acgaagatgc caggaaacgc aacgaattct gggtggtcat cagcctgatg
    50521 atccggatga tcggccagcc ggtttggggt cagaaaatca tcgtggaaac atggccgaaa
    50581 ggggtggata agttatttgc ttacagggac ttcaagatca tggatcacaa cgggaatgtg
    50641 atcggggcgg cgacctcggc gtggatgatc ctcgaccagg aaacgagaaa gccgaagagc
    50701 gtcgacctgg tcaaaccggt gctgcacctg gccacccacc aggacgtgct cgactgcaat
    50761 cccccgctgc tacggccggt gaaagagatt gcgacgaccg aaccccgcaa ggtgcgcttc
    50821 agcgaagtcg accagaacgg gcatgtcaac aacatccgtt atctcgactg gagcgtggat
    50881 gccctgccgg aggaatggca caaggagcat cgcatccgta cgctgaccat caattaccta
    50941 tcggaggtca tggccgggga gatgatgatg atctcgaccg gcaaggcgga agggaactcg
    51001 gttgtggtgc agggaaccag ggaagacggg aaggtggcgt tcaggacatt aatcgatttt
    51061 caggaatttt gagcggaagg tggtagtttg cgtctttact tcgataacat aaatccctgc
    51121 cggcaaactg gaacaataca ctgcgaattt caaatttcca atttccagat tcatcagcgt
    51181 ctctccaagc gaattaaaca gtttgatctc ccggattaaa tcttcagatt cgatccaaag
    51241 ccagtttgat gccggattgg gataaatttt caccttatta tcctgttgcg gtgaatcgac
    51301 acccaggcaa agcgtttcgc atacttcggg gttatcgtcg gattcacatg tgtcgttgtt
    51361 ctcagtccag actgccgata ctttgtaaca gtatttttct tcaaggataa tgtcatgatc
    51421 ttcatagttc catgaaaaag tcgtatcgag aaataggtaa tcgccgccat cggtgttacg
    51481 gtagatttta taaagccatt tatcattcgg aaatggcagc ccgggaatta catggcatgc
    51541 gatattccag ttgaaatcga ggtcgtcact gatatctttt aatgtctgcc attggtcctg
    51601 atagtagatc atgtttccaa atccatcgat agccggaccg tcatcaactc ctccagggta
    51661 accggtctgc gcattgacat aataaccgac ccagagatct tttgttacat caatagttac
    51721 agtatcaacc agtgtcacat agttccattc acctatcacc gggtattgaa caggctggtc
    51781 gataagcagg gagtcgggtg cgcttccgtt ccagatcctg atccggtaat ccgcgtttac
    51841 ttctgcaggg acaaaagcaa cttccgtgat tacggctcca gggaaaatga tcaactgagc
    51901 cggttcccac cttgaggcaa actcaaattc agccggttca cccaaaccga ttgaattacc
    51961 gctgaatacg ccatcatccc agttgaacca gccatcaaac catccccatc ctccataacc
    52021 atcccattca agagtattgt aatccggatt ttcttccagc cacacatccc agagcccatc
    52081 acaggtgcga taaatgtgaa tattatccaa tgcccatcta tttattgcgg acgaattttc
    52141 accggtagcc aggaaccgga ccttgaaaac tttattcttt gctgttatta aaatccgctc
    52201 actgatccaa tcaaaactgc cgtcaaaatt gctatattca gatatagcat tccaggtttc
    52261 gttggtccag ttccacacct gcaagaataa tttttctgta cccgtcgatt ggtttgcatc
    52321 cagtttgatt tcgaaatcga gccagattct tccgttcgtc atccccacag ccgtcatggg
    52381 gaagctctcc agggcgattt cataaccggt cactgccgga ttgccaagga atgctgcgga
    52441 aggtgcagga ttgccttcat cgttttcgat aatccagttt tcgctatcgg tcgtccattg
    52501 cagcgtttca aagctgcccg acgaccagtc ttcgagtacc ggcagctcca tcccataaaa
    52561 aatttgtgca gtatcgcagg cactttcgct gatccattca ttgtagaccg ctttggcgca
    52621 aaagctccgg tttcccgggg tgagggttgt tatatcgaag ataaagacgg caggttcggc
    52681 cggaacgagg accggaaggt tatcgtaaaa ttcgtcatcg accaggatat tgtaagaaag
    52741 cagctctacc ggctctccgg gaagcgaatc gggcgggaag atggtaaggg tatcgttctg
    52801 gaaaacgaca accgaaggcg gaggataata accgccgggc tgggcgaaga gaggagaagc
    52861 cgtcacgatc agaaggcata ccgcaaagta ccgccggaat ttcatggtct ggtattttgg
    52921 taataaagat acgaaataag tataatcggt tagaatgatc ctgtatttcc ctgaatttga
    52981 aaatatttca atgattcgta ttgaattcag ttgttctttt ccttaaattt accctgtcaa
    53041 gatatttctt tcctgaatta aaataaattt atatacctgg agcccggaaa ccagccatca
    53101 aacggggcgg aaccgaaaag ccacacgagt aggacaacca aaatccaaac taaaatggaa
    53161 gaccagaccc aattccagaa ccagaaccag aatccttacc agaattacaa tgccatgcag
    53221 aatcagcaac cgatcccggt cggcgactgg ttcgtgacca ttttgattac tgcgatcccg
    53281 ctcgtcaaca tcatcatgct gttcgtctgg gggttcagca gcagcaccca tccgagcaaa
    53341 gccaactggg caaaggcatc cctcatctgg ttggctattg ttatcgtgct ttacatcatc
    53401 atttttgcca ctattggcgc ggcattgttc agcgcgtaca gatgataaaa ccggccgggg
    53461 aaccgaaatc acatctgtat ttgctattca ggcttctctt caatgaagac atgaccctga
    53521 tgatttaaat cgggcccttc ttcgtaatag tcccaatctg tcctggaccg gatcaccgga
    53581 acagtggcag gatttgcatc tatcctatgg ccggagttgt cgttcaggaa tatcctcaca
    53641 taaccgtatt ttaccgcatc ttcgaccgat ttccagctta aatcgggctg aaacttcaag
    53701 gaaaaaccat tgtcctgcac ctgaaaatcc ctgatgccgt tgttaagcgt ataaactacc
    53761 agacttccgt taacaccgat ttcaacactg actgatgcct ggtctatttt gaggacttcg
    53821 ggatatattt ctacctccat tgccgccagg cggtcatata atagcatccc tccgaaacgg
    53881 tccagtgcaa tgaattcagg aaacggacac cctttgtcgt cccatgtgcc gatgtattgc
    53941 tgaaattctt ttcctccaat agtataaatc ctgcatttct caatatttgg cgggggtaac
    54001 acaaatgatc cgatatcgtt gtgtgtttca tcccaaccgg gcgtatagtc cgcccatgga
    54061 tacgggggct tgaaatcagt cacccgggcc ttcgtctccg gcaaggcatc cagaattttg
    54121 ccgctatttg cacctgccct tcctccgtaa tcgtaatgat taaatatatc ctccgcaatt
    54181 atttttgcat acccctggaa tttttctcca tcacctgaac cagcccatga caagggagga
    54241 acgaaacttc ctgtaaaaac aggcggtgat gatccgtcat tatcgctgcc ctctttcaat
    54301 accatgtcaa ctgttatttc agatttgttt ctgaccccta cttttaccct gaccgattcc
    54361 ggattgatct gcgtagcctg ctttaattct cccaccgaaa gagatgggcc aaaggtcact
    54421 tcaatgtagg tctgacggca tgcctggaat agtccgggtt cagcatttgt cttcaagaat
    54481 tttctgttcc ggtcgtcgtc tgtttcttcc cacatggcct cataacttaa tccggtcaac
    54541 acctgcccgt ctttattttt ctgatatatg ttgatcttac tggcataagg gggatgcctg
    54601 acaatatcat gaaataactg gatcaggccg gcggaaaatt cgacggaaat ttttgtgcaa
    54661 ttgcccgaaa gttttatgta gccattaata ttcgggtagt tcgacggcat atcccagaca
    54721 ataaagggga ttatcggaac atgatctatt tttactttaa ctgataaagg gggaatccat
    54781 cttgtattta gtgaagcggc ccggatgtta taatttacag gaacagggag tgcatgaagc
    54841 cccaaggcgg tatccaaagc cggttggcca aacctggagc gaaaatattc ggagccctgg
    54901 ccagccgaat cgaaataatt gaaaagtttt ccgtcagcca atccgacatt tttccagtcc
    54961 ctgatactca gcccggaaag ttcatgtgtc ctggcatcct cataaagatc ggccagcttc
    55021 tcaaatccaa cagcaccggg aactggttcc gggattttaa cccctgctgc cctccagacg
    55081 atgtcaggta taatccggat tttgcggatg tttttcatta attcatccag ttcttcagtg
    55141 ctgaaactgc ttccgggatg gtcaatacgc cggaaatgat cgggctggca tatgtcctgc
    55201 aacatatgta tgacatggcc gagacgttca tatgccgctt ccttggaaat cattgaataa
    55261 tcataagagc ggacagcccc ttcccagttc catggctcat tacccaggta aaaatcaccg
    55321 ggattcttct ggacagatcc cgaataagcc cattcataag cgctgttgaa gtccgtcatg
    55381 tcaaaactgg ctggcggtgg cgtatgatgt tttttataaa gatttactac ccagggagta
    55441 agcaaatatt ctttgcaaaa aaatccccgg ttaactccat aatggttata ggccctgtcg
    55501 aaatcgtcct ccattttgtt gacccttcga ccctgattga taatcatccg ctccccgaat
    55561 aattcaattt ctttataagc agggctgttt ttgaggagga tgagattgat agcttccgag
    55621 gtgattgtat tatgagtcag ttcgcaccat cccacggtta ttgcaatgat caaaaggacg
    55681 attgcagtcc tctttaatcc ggctctgaaa tggcccctgc tatttttcat ctggcttgtt
    55741 ttcattttgt atcctccttt ccattttaaa agactttggc gcttgatctt taaatacagg
    55801 caacaggatc atttcggctc tttcaagaca tttaagatga atattgaaat acacagtgcc
    55861 agacaggtat ttttcccagt taaatggccc atcggtcaca ttcggattct tataaaaatc
    55921 ttttgaatag tcagtcggtt caatcctgtt gtaaacctca gtatgaatgc atataagttc
    55981 ttcttcaccg gtttgtaaaa cagtcaacgc ctcaaagtcg cctttgaaat tcaggatcag
    56041 ctcgtcaata ttccgattca tttcaccatc cgtgacactt attttactcc aggtatcacc
    56101 ggtcactatt aaactggtgg gatctttcaa agctgacagg cttaccaaca caggtacagg
    56161 aatcatgcct ctatcattgt cccatgttaa attgcctgaa ggagtcattt tacaggcgat
    56221 cgtttgcgtt tccccctttt catcggtctg gaaaatgata tctccatttc ctctgaaatc
    56281 aagctttaca gcgtattgtc cggatacggg ggctccgtct tcggataaag tatccgaggt
    56341 gacgttgtaa taaatgatcc ggctggatac aacatcccat tcaatgggag atgccggaat
    56401 gggtttttct tccggaagaa aattaaaacg ggcgatttca ctctccccgt tattccggaa
    56461 aaatgatttt tgaagcggat cggatgctgt aatggagaac gcataagttt ttccgggtac
    56521 cagcctggga tcggatggcc cgtaaagata attcgttgaa gcaatgattt tctcgaagaa
    56581 taccggctgt gtggccgatc tgaaagcatc attgatattc ctgtcggccg gaagtacttc
    56641 gatgattttt aaggcaaacc gggttgagtt cggtgccccg ggaggccagg tccacacgaa
    56701 gttaatattt tgaaaatcgc ctgcgaagat atcctggccg gatacgggaa acagaatcac
    56761 cggtggttca aggtccgata ccgtgaacgg gttgctgcag cccagcgggc tttctgatga
    56821 taccgtctct cccgtgaggt agtcaaaggc ctgcaggcag atcgtataat tatcttccgg
    56881 aagcccctga ccatgaacta gttctgaaag tttgacaccc tggcaaacca ggtgctcttc
    56941 atcgaacacc tgctcgaggt
//
LOCUS       DAOPNH010000002        56516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_463_length_56516_cov_7.761605, whole genome shotgun sequence.
ACCESSION   DAOPNH010000002 DAOPNH010000000
VERSION     DAOPNH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_56516_cov_7.761605"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKZ74_00215"
     CDS             <1..193
                     /locus_tag="PKZ74_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPM91198.1"
                     /translation="YDKGQTYVSPTVENAVNKTYPISRPLYYYYLNNVSASVKPFIDF
                     ILSAEGQKIVKEVGYVPVK"
     gene            218..1423
                     /gene="pstC"
                     /locus_tag="PKZ74_00220"
     CDS             218..1423
                     /gene="pstC"
                     /locus_tag="PKZ74_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPM91199.1"
                     /translation="MRKYRKIWEKLAESVLFSSSTVTSLTVILIIVFLWREGVGLFNS
                     KPVDKGYVFALNQANPVNTLSAKQLKDIFNQDITNWKEVGGANDTIILLTINEIPNYF
                     TEEEMGTDLENLPVLIDRLILENSGAIVFISENYMPQDFHGRILDVEKISLKEFTFGT
                     EWYPTAEPVPLFGVWPLILGTLLVTLGAIVFALPLGLATAIYLAEVSSFRMRNIIKPL
                     VELLAGIPSVVFGFFGLVVLVPLIQQVFSLDVGETALAGSIILGIMALPTIITISEDA
                     LRTTPKSLKEASLALGATRWQTMVRVTIPYARSGITTAIILGIGRAIGETMAVLMVTG
                     NAAMIPTSFLMPVRTIPATIAAELGESPYGGLHFQALFALGIILFLFTLIINFFIELV
                     KGRKHIKTL"
     gene            1423..2289
                     /gene="pstA"
                     /locus_tag="PKZ74_00225"
     CDS             1423..2289
                     /gene="pstA"
                     /locus_tag="PKZ74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPM91200.1"
                     /translation="MMAMTKKRIIQKIAFSAFGAVSFIVVAVLFIILGFIAFKGAGVI
                     SWEFLTAMPEQGMTQGGILPAIIGTACLMVGSMIFAFPVGVLSGIYINEYLKDSFFKR
                     FVRMMTDNLAGIPSIVFGLFGMALFVNYLDFGDSIIAGSLTLGLLVLPVVIRTTEEAL
                     KSVDDSLRVGSLALGATKLQTIQRVVLPVAFPNIITGLILSIGRVSGETAPILFTVAA
                     YFLPRLPNSIFDQVMALPYHLYVLTTSGTNIEESRPMAYGTALVLILIVLLINVMAST
                     LRKYLGKKVKMN"
     gene            2301..3053
                     /gene="pstB"
                     /locus_tag="PKZ74_00230"
     CDS             2301..3053
                     /gene="pstB"
                     /locus_tag="PKZ74_00230"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPM91201.1"
                     /translation="MYKAEAKNVNFYYGDYLALKDINLSFPDKTVTALIGPSGCGKST
                     FLRLFNRMNDLIDHVKLEGEVLVNGENIYSKDIVIDELRKSVGMVFQKPNPFPKSVFE
                     NVAYGLRVNGVRDKNYIEDVVVNSLKKAALWDEVKDKLKKSAYELSGGQQQRLCIARA
                     LAVEPTLILMDEPASALDPISTAKIEELIHELKQQYTIIIVTHNLQQAGRVSDFTTFF
                     YLGELVEHDVTKKMFLNPGDERTQNYITGRFG"
     gene            3092..3769
                     /gene="phoU"
                     /locus_tag="PKZ74_00235"
     CDS             3092..3769
                     /gene="phoU"
                     /locus_tag="PKZ74_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPM91202.1"
                     /translation="MNHLEVELKNLKSSLLEMWSIVISQHEKAKMALDNHDKELANEI
                     HTNEKLLDAFELKHDMDCENLLVLNNPVAVDLRFVLAVLKINYNLERIGDYANYIANT
                     IRKSETPFDNKLLKDLNIGQMFDTAHLMMVGAYEAFDKCETSHVRHFSELDNKLDQWN
                     KDSFRILSEYILKYPTESKQALELFSIIRKLERVGDHNQNIAEEIVFYLDARVIKHKK
                     LHHKPNN"
     gene            3778..4830
                     /locus_tag="PKZ74_00240"
     CDS             3778..4830
                     /locus_tag="PKZ74_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91203.1"
                     /translation="MIRFTNPNRLALLISAANSLFISMVYGVGIMGNMIPFSWPFFFI
                     INALLFLFSYFFIRYIIQNFIFQKIKVIYKIIRSLKLSRTEKLEWRSNLGEDMIGQVS
                     QDVMDWAENKTAEIEQLRNIENYRREFLANVSHELKTPISNMQGYISTLISGGIDDPE
                     INRDYLQKTEKNINRLIELVDDLDIISSLESGEVNMDKSKFDMRALTREVFEFLEDSA
                     KSRGISFLFGNDDNNPVFVYADRYRIRQVLVNLVDNSIKYGRDGGRTKVSFYDMDDNY
                     LIEVSDNGIGIEEQHIPRLFERFYRVDKHRSRTEGGTGLGLAIVKHILEAHDQTVNVR
                     SSPKIGSTFSFTLRKG"
     gene            complement(4884..5231)
                     /locus_tag="PKZ74_00245"
     CDS             complement(4884..5231)
                     /locus_tag="PKZ74_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM91204.1"
                     /translation="MKKAINIFAIVVLSSFGIAAFADNNPDLTPKSVKITGQVVDKVN
                     QEVLAGALVKVEGTDIEVYTDFDGNFSIEGIAPDKYTLKCSLISYTECEKEICIDTAG
                     ETVEIELENFTSK"
     gene            complement(5340..5801)
                     /locus_tag="PKZ74_00250"
     CDS             complement(5340..5801)
                     /locus_tag="PKZ74_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91205.1"
                     /translation="MYKLLINSIFAMLMASVVFGQAQQGADKLYYDKDGRLFTGITRD
                     YYPDSALHAEFEIQNGVLSGISKVYFNNGKLEEIRSFREGMMHGKWEKWNQQEVKIAE
                     ANYTDNLKDGKWYIWDENGTLRYDMTYIKGEKSGVWMMYDEKGKVISQKSY"
     gene            complement(5878..8769)
                     /locus_tag="PKZ74_00255"
     CDS             complement(5878..8769)
                     /locus_tag="PKZ74_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91206.1"
                     /translation="MRAIKVFAALVFLILAASLQGQNGTIRGTVYDDATGEYLPGVTI
                     FLEGTTLGTLTDLDGKFNLSAPAGNYDLRVSFISYEAMIIKGIRVEEGDVTLFDNLRL
                     KEATVELTEVVIAAQGIRNTENALLTLKRKSANVIDGISATGFKKIGDSDAAASMKRV
                     TGVSVEGGKYVYVRGLGDRYTKTILNGVDIPGLDPDRNTIQMDIFPTNIIDNIIVHKS
                     FSADLPADFTGGVIDIAIKDFPEERKGSIAFGLAYNPGFHFRSDYLTYEGGKTDWLGY
                     DDGTRAIPATENIPQFVEAYLDPNGDAGLRYKEILGGFNTTLAAREQTSLMDYNIGAT
                     YGNQHVGNIVTLGYNFSLSYKSNTEMYKNAEYGRYGLTDSNTFEMDVREYQKGNFGVS
                     SVLWSGMAGFAVKTKASKYRINIVHLQNGESRAAVFEYQGSDQGSIFGGFQHNLEYSQ
                     RSLTNLIISGKHNLAGSEWEIEWKLSPTLSKMYDPDIRFTRYKADNGKFIIGTETGFP
                     ERIWRDLEEISYAGIVSVTRQFDLWKRNARVMFGGAYTSKERDFIIRNYAINIRNVPL
                     TGDPDEIFSPENLWPYNGNLNYGTSYEAAFYPNNANRFNSGVRNMAGYVSAEVSPFNR
                     LKAIIGVRVENYVQRYTGQNQQGTIVPDNEKVLDNTGFFPALNLIYALNEQQNLRFSY
                     ARTIARPSFKELSYAEIYDPISGRTFIGGLFRDADEQLGIEYWDGNLVSTDIDNIDLR
                     WELYKPNGQMVSVSAFCKMFDNPIEIVQYAVQAGSFQPRNVGDGQVFGAELEFRFNLI
                     NISQELRNLSLSSNLTYTRSFIRMSNVEFQSRVDNARVGETVGEYRDMAGQAPYIINL
                     GLSYDGGEKGFWNGFEAGLFYNVQGKTLQYVGMVDRPDIYALPFNSLNFNSNKSFGKN
                     NKFQVGIKAENILADWKDAVFISYMATDQYFSRLSQGITFQIRFSYSFF"
     gene            complement(8962..10401)
                     /locus_tag="PKZ74_00260"
     CDS             complement(8962..10401)
                     /locus_tag="PKZ74_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91207.1"
                     /translation="MKKILFYFVIFSSLIAFLTSCDKDENNMAPAVTAPANANVEINA
                     AIDLTFNFTAEAGFKSSAVTATNGTASVKTDGTAGSTSGSVVVTFTAGANAGAGSVVL
                     TLTDNDDQAGSATAVLNVVEEELIISVPGNITENTTWETGKVYILESRIAVVSGATLT
                     IEPGVIVKGQAGTGANATALLIARGGKIMAEGTADAPIIFTSVADEIEPGMIESPNLD
                     PVLNGLWGGLLILGNAPISADAASMQIEGIPPSDQNGLYGGDNAADNSGVLRYISIRH
                     GGANIGEGNEINGLTLGGVGSGTVIENIEIVSNQDDGIEWFGGTVSVKNAIIWNTGDD
                     AVDTDQAWAGTLDNFIVVNPGDECMELDGPEGALDNAGHTIKNGTVYAGNAEGLVDLD
                     ENTRCTITGIYFFGVAEGQDFDLNPAVLTCSNLQATLPGGAVVTDYFKGGSDAYVTIV
                     AEGANTVGADVTKFQTWSWAAVSGALNGF"
     gene            10696..11469
                     /locus_tag="PKZ74_00265"
     CDS             10696..11469
                     /locus_tag="PKZ74_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM91208.1"
                     /translation="MSNEPKSIHEFDFNLICEYFSSVERQGPGSPEMTIKALSFIDNL
                     TADSKIADLGCGTGGQTMVMARNVPGKITGIDLFQKFIDLFNQNAGNLNLQHRVKGIV
                     GSMDNLPFQPEELDLIWSEGAIYNIGFERGINEWRKFLKPGGFLAVSEASWFTPERPA
                     EIEEFWKDAYPEIDTIPNKVLQMQNAGYVPVATIVLPENCWIENFYAPLVKAREIFLQ
                     KYAGNKTAEEFIASELHETQLYFKYKAFYGYVFYIGKKI"
     gene            complement(11515..12597)
                     /locus_tag="PKZ74_00270"
     CDS             complement(11515..12597)
                     /locus_tag="PKZ74_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91209.1"
                     /translation="MKVLTSLIFLLFLLPGSIYGQGSSKAAYIISNDADTLFGAGNMG
                     PEQDFCLFREIGSDDARVYNPDEISAFRYINGKYYVSKQIKNKSGEVKWCFLEFLVDG
                     EIDLFRIRNAHRYFIKRDNQDLLELDDRNTEVVNSDGRYYLKKNRTFNGLMRYYMSDA
                     PAIFPEIDKIEDITQKSMVKIAIDYHNIVCKDAECINYTKSISKFIVSWKLEAVSGAT
                     YHNAYYSPYYGLQVHLGRKENIEHMFLKTGLIYSDKIYWAKDNTSSGERQYKIRIPLS
                     FQYVFCKGVVRPTVAIGWPSGFLINLSVQAGVLLSISKQFEIALNGSVDGLHNLMLDR
                     NQEYFNNDLAHTFSFGVIYNFSANKE"
     gene            complement(12889..14082)
                     /gene="kbl"
                     /locus_tag="PKZ74_00275"
     CDS             complement(12889..14082)
                     /gene="kbl"
                     /locus_tag="PKZ74_00275"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPM91210.1"
                     /translation="MYGKLQDWLKNELKSIEEAGLFKKERIIVTPQGAEIKVSGGQEV
                     LNFCANNYLGLSNHPKLVKAAKETLDSHGFGMSSVRFICGTQDIHKILEKKIAEFFGT
                     EDTILYAACFDANGGVFEPLFGEEDAIISDSLNHASIIDGVRLCKAQRFRYNNADMAD
                     LEEKLIEASKTARFKIIVTDGVFSMDGNVAPMDKINELAKKYDALIMVDECHSAGVVG
                     KTGRGVTELFNIRGEVDIITGTLGKAFGGGIGGFTTGRREIIELLRQRSRPYLFSNSL
                     IPAAVGAGIAMFDMMAETNELQDKLHANTDYFLEKMKAAGFDIKPTKSAICAVMLYDA
                     KLSQVMAEKLLAEGIYVIGFYYPVVAKGLARIRVQISAAHERHHLDKCIDAFTKVGKE
                     LGVIK"
     gene            14340..15332
                     /locus_tag="PKZ74_00280"
     CDS             14340..15332
                     /locus_tag="PKZ74_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPM91211.1"
                     /translation="MKKLLKIFLWFVLVIVIIIVIAVGYIQLTWNKTFDAPYPDITAS
                     NDPAMIERGKYLAYGWSHCAACHVPADKVREVENGLEMPLIGGWDEEIAGFGTFRAPN
                     LTPDPETGIGRMTDGELARAIRYCVKHDGKLLPPFMVFQGLSDEDLTAVISFLRSQEP
                     VKNQVAPSDYGFVAKAVIAFGLLKPQGPKSTPPASIRPDTTAVYGEYLATSVGNCLGC
                     HIKIDQAGNQVNPDFAGGGIFPPTAFSKGYGFVSPNLTPDKKTGAIADWSEEQFVNRF
                     QGGRLHEGSPMPWNMYSRMSVNDMKAIYRFLHTLDPVGFSVEKTVYQPGEKMPQ"
     gene            complement(15326..16195)
                     /locus_tag="PKZ74_00285"
     CDS             complement(15326..16195)
                     /locus_tag="PKZ74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91212.1"
                     /translation="MIRKYLLQQYPYEENKWKIILPISIFILFFMPVFQPFGLSEFQS
                     PDKLLILAGYGLVTFIILMIDMILIPVVFPKVYSEEKWTVFKEMLNFLLILFTIGLGN
                     LVYSHFYIGFRLTFQNILIFQLFTVAIGIIPVIAIVMIKQSYLNRKNAVSAGMITSGL
                     HPRPDVETQFSALKIFSEGGKEELEILFDDLVFIKSEGNYISTGYIKKGKMVRTLIRN
                     TMKYAEDLIAPIPAAYKCHRSWIVNLDKIEKVSGNSQGLKLTLAGFEEPVPVARNLAS
                     DFKRVMGERQATY"
     gene            16254..16949
                     /locus_tag="PKZ74_00290"
     CDS             16254..16949
                     /locus_tag="PKZ74_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91213.1"
                     /translation="MLLIRLSGHSCAGKTRLVNALPKYGITLPKVLRYTSRAPRPEEI
                     EGEDYFFRSREYIEALPGKDFLKGPVRNMLQAFDLNAIANLLGNHTGVIIEIYPGLWP
                     ELVSRLRIRLGNNLKTASVFMTAVDPERIRQFKKKDEIAGFISSEVYKMLLFRNKDEI
                     EDIRIRAYSAALEITGALSPQGRKEYDRIIFSAPEGPDGEDEWTRGPEPVGQALIALN
                     KFREFLYEFEKPY"
     gene            complement(16954..17634)
                     /locus_tag="PKZ74_00295"
     CDS             complement(16954..17634)
                     /locus_tag="PKZ74_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM91214.1"
                     /translation="MIEIKNLNKYYGDHHVVKNARMVFEKGCVYGLVGENGAGKTTLF
                     KCIAGLEKHDGEIITDYGHLKDHLGYLPTEPYFFQKITGMEYIRLLCNARRIKAENIH
                     EKNIFDLPLNQYASTYSTGMKKKLALLAILMQENEVFILDEPFNGVDIQSNILIADII
                     LRMKELGKTVIISSHIFSTLSETCDEIFLMKEGAIIRRVLKADFPALEYEMKQILVGD
                     RIEKLGLK"
     gene            complement(17631..18548)
                     /locus_tag="PKZ74_00300"
     CDS             complement(17631..18548)
                     /locus_tag="PKZ74_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91215.1"
                     /translation="MKLYFLIQFKMLNRHLAAFGIHPVIGCVLILAAFTGSSVLIFEK
                     TAFAEYLYLAASLSFLARLSDPARNQFLRSCFGKKDYRVVRMLENVIVAAPFAAFLVI
                     EKYYFHALAALFLAGFMSLTIVGNRSGFTIPTPFGKRPFEFTVGFRNSFPVIVLAWFL
                     TVMAVISGNFNLGIASLILVFLVCFSYYLNPENEFYVWIFNSRPGIFLFGKIKTALVY
                     ATFLTLPVSLVLSVFYSDKILIIGAFQLLGYIYLGAVVLAKYSSYPHPMNLPQFIILV
                     VSVWFPPLLLAVIPFFYRKSSIRLKTLLA"
     gene            complement(18545..20245)
                     /locus_tag="PKZ74_00305"
     CDS             complement(18545..20245)
                     /locus_tag="PKZ74_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM91216.1"
                     /translation="MPRTIAIIAPFIDTRQFNMDFTLRLARDNRELTLATFYNWAGNG
                     FSGVDLPRGIDYGEDIPAAGFYLQGILHAHGYETILTNKYDDLTLEALARKEIFAVCI
                     STTMIITTDSLVALCASVKRAMPGVPVIAGGVNIWKNYFQYERHLSSPEDFPLHSEML
                     FHPSNSAIKADVLVVAQHGIGSLMRVLEVIESEGFKGFKGGKGGKGFEDWGIGGLSNV
                     PNLCLPGEDGFVFTGMVEEKVDYDEDFTRWDLISEMPEKIPLRTSIGCPYRCRFCDFC
                     SLFPKIFLRSPESLCKELSMVKSRTGSTPAMIHVTDDNVFITRKRLFEICGTLSKSGL
                     NHWAGFMRGGEYSEDEMKAILDSGLMMGKMGVESGDQGQLDRMNKKQKIDRVKRGIEQ
                     FDAQGIGVLMTFVVGFPGETEETLRNTIDFMNNLALTHLGVSYQVYPLLIFNLSELAE
                     PAIRKKWKIEGFMDQWSHYSMNSEQADNACREVFRSVTNVPYNYSEESNFFNRGMFDP
                     DTRRKLFHLRQQLTVHLLDRENMMDNEHIIKEMARLMELPAEETGMVFTDEIVIPGSK
                     "
     gene            complement(20305..21147)
                     /locus_tag="PKZ74_00310"
     CDS             complement(20305..21147)
                     /locus_tag="PKZ74_00310"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPM91217.1"
                     /translation="MNNKIYPCLWYDSKANKAAVLYREAFEDVVILEENPIVITLEIF
                     GQKFMLLNGGPMYQPNPSISFMVTCKTTEEVDHAWEKLIVEGKPMMPLDAYPWSRRYG
                     WVQDRFGISWQLYLGNPKDTGQKICPTLMFTHQQFGKAEEAIRFYTALFPNSSVGGLL
                     KYSANDDDEEGKVKHAQFTLDGFGMMAMDSSFDHPFRFSEGVSIAVDCDTQDEIDHYW
                     NKLTGDGGEESMCGWLKDKYGVSWQIVPSILPALMADPEKSQKVINAFLKMRKFDLAT
                     LLAI"
     gene            21245..21763
                     /locus_tag="PKZ74_00315"
     CDS             21245..21763
                     /locus_tag="PKZ74_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM91218.1"
                     /translation="MDFLTEEEIIAGCLKNDRKAQKALYDQYKSRMFTLAYRITNSFE
                     DAGDVLQDGFIYVFENLDSFRGNSRLSTWIHTIMARTALKKIRERIVFSDLTENEGPE
                     TIDWGGEIDVQLLEQAIHGLPDGYRSVFTLYEIEGFKHTEIAEMMGISVNTSKTQLFK
                     AKRMLIQKLRGN"
     gene            21764..22363
                     /locus_tag="PKZ74_00320"
     CDS             21764..22363
                     /locus_tag="PKZ74_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91219.1"
                     /translation="MEKNHDILKKALGRMKVYDPPEEVWNAFVKITGEGDRALGFRKL
                     TVYSPPDSVWDHIARRLALKDRGKGEKRSIAILKWTAAVAAIVIPFVIIFSVTGDREI
                     RTHYSEEWVVMADIRNNTSDDSLSYHVIDNKCRKKPEICQSDEFLGLKKELAFLDNSK
                     KTIINRLNKYESGKDLEALLIRIDLEREEILGKLIAISN"
     gene            22365..23147
                     /locus_tag="PKZ74_00325"
     CDS             22365..23147
                     /locus_tag="PKZ74_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91220.1"
                     /translation="MKRGILYMLFFFILAGNSFPQVRVQVVSREINKTIAWKEGMSLV
                     VNGDNAEIYCSSGFHQDIGISMTIISKHEERTVAEADLEKMKWLVEVKDNTCFVRNYI
                     ELSRNQARPESALKVILRITVPADCPVSLHNYFGKTEISGLNSEVTLNNEYGPVLLSD
                     LNGNTTVNSVFGDVTINGSMGHTRITSTHSQIVLRLNALYQYGFNFELTDSEIIKPEE
                     MIIIYDKKEKGKITGQIKGQNSGPEISVILKNCSLTIEQSNN"
     gene            23247..24470
                     /locus_tag="PKZ74_00330"
     CDS             23247..24470
                     /locus_tag="PKZ74_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91221.1"
                     /translation="MPSFHRNKPVLPLYLIIILLSLLGLKPGKTFAQEENDSPVNYIN
                     SMEYAFMMHEDTRWLLKADLVVKNEYVNMNPFKISFEHRIYKSLTANASIDHVNMNMP
                     GDHMYFLPLNFSIEPRWYYRLSKRMKDGNAASSLSDNYLAMGVSYTWLLNYNQVESPV
                     DRRYVSYYAKWGMQRRYLKYGHIDMGIKAGMMDAAGNGFSPSLVLNTFVEMGLGFSKD
                     RQAMDRERLCPFLRCYEADRFIVKTNLTDLVNLGIFMDQKWVELSPQVALEHKLGTSS
                     FSLNASIGGTLGYAEFSEADKVLTDRYWIGEVSLEGRYYYNLKKSILNGLSGNGLSAN
                     YLAAGGGYCVTDGQYDEMIKETGPRLYLATGWQRLLNRHLYFDIQVGANYNFRPNRAG
                     NVYEPMFQYGLGYRF"
     gene            complement(24512..25003)
                     /locus_tag="PKZ74_00335"
     CDS             complement(24512..25003)
                     /locus_tag="PKZ74_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HPM91222.1"
                     /translation="MNIRFTEITEADFAVIKGIYDYYILNSTSTYYTELISIDELKQF
                     IPLNSQRYKSYMIEAGGECCGFCYLSQYKKRQAYDRTAEISLYLKNGYTGRGIGRIAL
                     DYLEEIAREKGIHVLLAIISGDNIASIKLFERAGYEKCGHLKQIGEKFHRVLDVLFYQ
                     RFL"
     gene            25111..26073
                     /locus_tag="PKZ74_00340"
     CDS             25111..26073
                     /locus_tag="PKZ74_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM91223.1"
                     /translation="MKNILVIGSVGQIGSELTMRLREVYGASHVVAGYRKTMPPKELA
                     ESGPLEMVDATDAAKMAEVVKKYKIDTIYNLAALLSAVAEGNPTMAWNVGVGGVFNCL
                     EVARENNCAIFFPSSIGAFGPSTPQDNTPQDTIQRPSTMYGVTKVTGELLSDYYFKKF
                     GVDARGLRYPGIISNVTLPGGGTTDYAVEIYYAAIKEKKFTCPLPAGTFLDMMYMPDA
                     LDAAIDLMEADPSKLKHRNAFNVSAMSFDPEIIAGEIKKHIPDFVMNYNVDPMKKAIA
                     DSWPNSMDDSCARAEWGWNPKWKLDNMTVDMLKVIGEKHAKGLI"
     gene            26164..27276
                     /gene="mnmA"
                     /locus_tag="PKZ74_00345"
     CDS             26164..27276
                     /gene="mnmA"
                     /locus_tag="PKZ74_00345"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPM91224.1"
                     /translation="MQIAALVSGGVDSSVIVPLLKEQGYDPVIFYIQIGMENEPGFMD
                     CPSEEDIEITTFIAKKYGCRFEIANLHEEYWNRVVNYTIESVSKGLTPNPDVMCNRFI
                     KFGSFEDKFGKDFDKIATGHYAQDLEKERQFFLGTSKDRRKDQTYFLGRITYPQLSKA
                     MFPVGGFEKEEVRAMAERLKLPSAGRPDSQGICFLGKVNYNEFIRRYTGEKEGDIVEL
                     ETGKILGKHKGYWFHTIGQRKGLGLSQGPWFVVKKDIPANIIFVSNGYDPVSQHKHEI
                     LLDDFLFINETPDRDYSVHQPVFFKIRHQPDFNEGTMRRIGGLGDLEIGGLEEGERYV
                     VQSVKSIAGVAPGQFGVIYDVEKKICLGSGVIAERE"
     gene            27520..28662
                     /locus_tag="PKZ74_00350"
     CDS             27520..28662
                     /locus_tag="PKZ74_00350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPM91225.1"
                     /translation="MEKFIMYNPVKLHFGRGVVSDLGDSISKYGKKVLLVYGGGSIRK
                     NGIYEEIISQLGKAGAEVFEYAGIRPNPVIEDVDAAASLGREKGVDVVLAVGGGSVID
                     SAKIISITIPVLHSGWDFIKGKSKPVCAIPLFAVLTLAATGTEMNRFAVVQNNLTKEK
                     LGYGDLLTYPKESFLDPAYTLSVPSDYTSFGITDLIAHCLEAYFGEGEATLSDRFVFS
                     IIGEAMEYGPQLMHDTGNYLLREKIMYAATCALNNLTVYGRKNGDWGVHSIGHVLSVL
                     YDVPHGASLSMVYPAWMRLHLDRAPERIAHLGMNLFGTTTAEETIEGFERFFTGIGSP
                     VRLSQYGLDTPENRAEIFRTLKANRAGGNHFRLSDQDYENLIQLMA"
     gene            complement(28670..32023)
                     /locus_tag="PKZ74_00355"
     CDS             complement(28670..32023)
                     /locus_tag="PKZ74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91226.1"
                     /translation="MARPILHHGNGWYRVMDKTAGSLKAQVLILIRTDYPYQNQYLIN
                     QFHEDFQLDPDAGLDTLKRDLNIFSEDGDFLFSLIPPDTWQTNEGIGLAVFGLAIIAF
                     ICMVLVLFYLYHLFDFFRRYPVIMFIAFCADAVVLRLAMIWFEIPGFLYQTALFSPLY
                     YATSFFSPSLGDLVINNILWLALAWLFYRTDLNIPIPKSEILKIAMGLFWMLINALLF
                     FLLIMALKHLVLDSNIELNLNNIFEADLYSGLGFLALSIAVLSCFFASAKLFSLLCKS
                     GMKTGSLFLISTITVILITLLFSQSLDHIAALVFIVIFCLYLLAFIYFSHRKRGFKNM
                     TGALVFIVLFSLTATFILDYYYSEKEKENRKILASKLASRRDPLTEFEFSRIRTDILK
                     DSVLIDLISKDVWAEKIDSLVTGHLQDHYFTRFWVRYELVLTICKKDEILNIQPGDYI
                     ANCYDYFNSRITSPFAENVGSGLYFLDDRMTNYNYLGIFKLPYGTKNRTDSLTVFAEL
                     FYKYARESGLGYPDLLIDGTAGMTGDKSGYSYAKYINDRLIYKNGEFVYPLGFDVYDI
                     PESNDYFIDNEGYNHYIARSANNNILIISKKNLTLLDLLAPFSYLFILFSLAILLFLG
                     GYTLFKGISQIEFSFSNRLQLAIISIILVSFLVLGMITRANIIHLYNDKNRDNLSEKA
                     FSVLTELEHKIGSEKAITEDLKEYIAEILHKFSMIFYSDINLYDVNGKLIASSRPEIF
                     DEELLSDKMNSLAYFRMTSGQSLLFIQNEKIGRQEYLSAYIPFRNTDDDIIAFVNLPY
                     FAKQTELQKEIGDFLAAYINVYVLLIVVAIMVTILVSRFITRPLQMIREKMARIGLGK
                     SNEKLYLKRRDEIGDLVEEYNRMIDELALSADKLARSERESAWREMAKQVAHEIKNPL
                     TPMKLSVQYLQKAWERKDPDWEKHLHRFTRTIVEQIDTLSEIASEFSDFAKMPAPVME
                     KIELTGVIRSAAELYFHHQNIRISIRQQKPAYFVKGDDKQLLRAFNNLIQNAVQAIGP
                     GPEGQIDISLSEEGSNYIVAITDNGCGITDEQAKKMFSPSFTTKSSGMGLGLAMVRSI
                     LNTMNGSITFESTPGTGTTFTLKIPALPE"
     gene            complement(32563..34434)
                     /gene="mnmG"
                     /locus_tag="PKZ74_00360"
     CDS             complement(32563..34434)
                     /gene="mnmG"
                     /locus_tag="PKZ74_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPM91227.1"
                     /translation="MFKEYDIIVVGAGHAGCEAAAAAANLGSSVLLITMNMANMAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVTDKTMIQFRMLNMSKGPAMWSPRAQSDRMLF
                     SIEWRKMLENTPNLDFWQDMVTGVLVKNGVATGVSTAMGHEIYSRSVILTNGTFLNGI
                     IHIGLKQFGGGRIGEKAAKGLTECLVELGFESDRMKTGTPVRVDGRSIDFSKLEEQKG
                     DSDPRRFSFTKTPPLTRQRSCYIAYTSREVHETLKTGFHESPMFTGRIEGIGPRYCPS
                     IEDKINRFSGKESHQLFVEPEGWETEEYYINGFSSSLPDHVQFNALRKIAGFENARIF
                     RPGYAIEYDYFPPVQLNYTLETKKVPGLYFAGQINGTTGYEEAAAQGIVAGINAHLKV
                     HRSEEFILKRSDAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQSNADQRLTPLAH
                     SLGLASAERYQTVMSKSEKIHEVVRFLQKTSTDPDQVNPYLVNAQTAPIDQKVKLATL
                     LLRPQISLDGLAGQIPFLQEYLGSVAPDRVEILEEAEIMIKYQGYIDKEGELAQKLER
                     LDSVTLKEDFDYHRLTALSFEAREKLSRIRPRSLGQASRIAGVSPSDISILIVHLGR"
     gene            complement(34490..34921)
                     /gene="ybeY"
                     /locus_tag="PKZ74_00365"
     CDS             complement(34490..34921)
                     /gene="ybeY"
                     /locus_tag="PKZ74_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPM91228.1"
                     /translation="MAKESNIFFFLEEISYQLKNKRKLRHWITQAVENEGHKINNLNY
                     ILTSDNILFQLNKEYLQHITLTDIITFDMSESEGELAGDIYISVDRARENAKEYHVSL
                     TNEISRLMIHGVLHLMGYKDKTNEERVVIRAREEFYLSLLI"
     gene            complement(34933..35415)
                     /locus_tag="PKZ74_00370"
     CDS             complement(34933..35415)
                     /locus_tag="PKZ74_00370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91229.1"
                     /translation="MNRPKEFTIGSTLEEMYRIEQFVDEISDEYLLYGSYFGNILMAL
                     TEAVRNAVVHGNGNDSRKRVYIRQEKVKAGLMLTVSDEGQGFDFKTALESAANLKAPE
                     TSGNGLFLMQKLSDEINFRNEGRTVEMLFRINGIDESIFNRRAECMNDFFKVYQKVNS
                     "
     gene            complement(35419..38775)
                     /locus_tag="PKZ74_00375"
     CDS             complement(35419..38775)
                     /locus_tag="PKZ74_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91230.1"
                     /translation="MTEDNYSILIRKLDEFIRKFYRNRLIRGAIFYVAIVTIAYLLIT
                     LTEFFGRFSPGIRTLFFYSFLVLSAGFLWYYILVPVIRLLGFGKIISHEEAAEIIGKH
                     FPEISDKLLNTLQLKKLQEHHTDEFPIELIRAGIDQKIEKLRPVPFALAIDFRKNGRY
                     LRYALPPVIIIILLLIMSPRVITEPSERIVKYQTVYLPPLPFTVQILNEKLESFQQED
                     FLLELKAEGEELPAELFVETGGVRYRMERSSPVLFRYNFRNLQESKLFIISNDLFKSE
                     EYQLTVYPKPIVLSFEISLDYPVYTGKSDEKSENTGDLIVPEGTVLNWRFFTRDTRNI
                     LFRWGTETSELSNPSSNAFSVSKRAHASVTYSVASVNQYLRNSDSLTYTVSVIPDAYP
                     SVSVEQVRDSIYDKRIYFRGLIKDDYGFSKLTFNYRLDPEGDADANDYPEIIAELPVS
                     KLHNQQQFFHFFELDSINIQPGEQIVYHFEVWDNDAVNGSKVTRSQPMIFRAPTLDEI
                     EEKTEESNQEIMEELENSIRNAKDLQKKVDELNRKLVDKEEIGWQEKQQIQELLDQQK
                     ELQETIEKIQKENELKSIQEQEYKEINEELVRKQQRLEELFEQIMTDEMKKMFEELQK
                     LLEEVDKDKVNEMLEEMKKDNKDMEKQLDRNLELFKQLEFEKKLQESIDKLKELAGKQ
                     DEQAKKSDDRKADAEKLKEEQDKLNEEFRQLRDDLDRLEQKNKELEEPHQLENTEQME
                     QEIQDNMEEGSDQLQQGKPSKASPKQKSAAEGMKSMASKMEQMQSEMYEEELGENIET
                     LREILENLVQLSFDQEELIGRTLEISVIDPQYPSVIEAQNRIRDDLVMVEDSLWALSK
                     RQQMIEPFITREIQDINHHVEAALEDLTERRKSSAAEDQQFVMTSVNDLALLLSETLQ
                     QMMNAMQMQCSGQCKNGSPKPGKGSMSMKSMREMQQKLNQQIQQMKQEGSTPAKTKES
                     RNSAQSEGFARMAAQQEAIRRMMGEYQDQMKEQGMGNSKELQEIMEQMERTETELVNK
                     IITQQTIERLKEIETRLLKHEKAELKREQEEKRESREGKDFNKGNPEQFLEYNKIQDK
                     EIELLRTVPPDLRPYYKEKVNQYFYYFEMR"
     gene            38895..40865
                     /locus_tag="PKZ74_00380"
     CDS             38895..40865
                     /locus_tag="PKZ74_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPM91231.1"
                     /translation="MLKRLVFLSILIPLTGILMAQYNHPEPDDAPYCYHVKSAAMAAN
                     EYYAPVVTNPLLEKYDVTFYFLDLNVENDTVAVSGSVTTKAIVTGTQMDTIVYELIDE
                     MTVDTVYVNGIQHSFVHNNDLILVPIEPIPNGVYFTTKICYHGTPPTGGFFSGVTTAY
                     STPFEKNVTWTLSEPYAAKDWWPTKQDLTDDADSVWVFLTTSAENKAGSQGLLTAVTP
                     VPGGKLRYEWKSYYPIDYYLISFAVADYQEYNIYAYPEGTEDSVLIQNYIYDHVNCLP
                     LYQVGIDRTAGFIELFSDLFGLYPFIDEKYGHCLTQLGGGMEHQTMTTIGAFNFNLVA
                     HELGHMWFGDKVTCATWSDIWINEGFATYSDYLAVSNLLSPAEDSAWLRSKHNQVKSA
                     PGGSVYVPEEELDNIYRIFNGRLSYSKGALLLHMIRFELQDDEMFFNVLRTYMEEYGD
                     SVATGADFREWLEYISGEDFTDFFDQWYYGEGYPIFDIVWNQNNGNLNVSSTQTTSTT
                     VTQFFKMKVPYHVVYSDGTDTTLILYQGENLETYSIPVSKEVEHIELDPEQWILHHLN
                     SLAVDIEERNNPLYFTVGPNPAHDYFRVFLLSNPEKEMQLSVTDLAGRTLLTQSITGR
                     SSAIDAGHLKPGVYQVIVSDGKGRMNRKILID"
     gene            40862..42397
                     /gene="gltX"
                     /locus_tag="PKZ74_00385"
     CDS             40862..42397
                     /gene="gltX"
                     /locus_tag="PKZ74_00385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPM91232.1"
                     /translation="MMKEVRVRFAPSPTGPLHIGGVRTALYNYLFARHSGGKMILRIE
                     DTDQNRYVPGAEQYIIDSLEWTGIRFDEGVHVGGPYAPYRQSDRKEIYRQYADELVRA
                     GHAYYSFDTADELDAKRKAAEASKQVFTYDSSVRNTMKNSLTMTGEEVQKRLESGEPY
                     VIRFMIPENTTLEFNDLIRGDMTVNTATLDDKVLFKSDGMPTYHLANIVDDHLMEITH
                     VIRGEEWLPSLPLHILLYRAFGWQAPLFAHLPLILKPEGSGKLSKRDGDRLGFPVFPL
                     EWTDPFTGDVSSGYRESGYFPETVVNMLALLGWNPGTEQEIFSMEELIGQFSIERVGK
                     SGSRFDPGKIKWFNHHYLITKTNEELTDLFCKVLEDKGIHAERSFVSRVVELVKDRSD
                     FVGQFWDQSYFFFRRPESYDADLARKKWKEDTPGLLRGFAEALPGLPDYRAEQIKLFA
                     ESYAAGKGAGLGQLMNPLRLCLVGGSFGPDLALICEMLGKQEVMDRVHLALNFLKSSG
                     TIV"
     gene            42402..42746
                     /gene="folB"
                     /locus_tag="PKZ74_00390"
     CDS             42402..42746
                     /gene="folB"
                     /locus_tag="PKZ74_00390"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPM91233.1"
                     /translation="MGTIAIEGMEFYAYHGCFHEEQIIGTRFTVDLFMEADTTSAELT
                     DDLSETVDYQSVYRVVSEEMAIKSKLLEHVARRILSRIGREFPQVGAARIKVSKMNPP
                     VGGKTERVSVEI"
     assembly_gap    42984..43083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(43088..45304)
                     /locus_tag="PKZ74_00395"
     CDS             complement(43088..45304)
                     /locus_tag="PKZ74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPM91234.1"
                     /translation="MKRFTIFFSLFILLSAPSLRADEGMWLPIFVERLNYVDMQKMGL
                     HLTAEEIYSINNSSLKDAIVGLGNGSQPGGFFCTAEVVSSQGLLFTNHHCGYDIIQKH
                     SSIEHDYLNDGFWAMSKEEELPNEDLSATFFVRMEDVTDSIVPFLSDTMSASKRSEKV
                     REITARLKKAANPDKKYFVNVTSFFNGNEYYLFVYEVYTDVRLVGAPPSAIGKFGGDT
                     DNWMWPRHTGDFSIFRVYTAPDGSPADYSKENVPMVPKHHLPVTLNGVEKGDFAMIWG
                     YPGSTDRYLTSYGVDFQINHFDPNIVRLLGEQLEIWKEDMTADREVGIKYASKYAGTA
                     NGWKFYIGELKGLKRLDVKSQKEELESQFTAWVEKDAARKEKYGDVLPGIKAGYEKIS
                     KSFGPFILAALGASSPEILGYAQNFSGVGGYLEGKDEAGLKAESESLREGAKEFFKDY
                     NAPTDQKVLARMMEIYSKEMPVEMQPQGFKDLVAKYKGDFKAMAADIFAKSFLSSEAK
                     VNAFLDKPSLKTFQKDPAIKLSNDLMGSMMNLSGDYRSANQEMGKYERLFIAGLREMM
                     PEKAFYPDANSTMRLTYGTVEDYKGADAVYYDYKTHLSGVMEKEDPANEEFIVPEKLK
                     KLFEEKDFGPYGCKDDLVTCFLTTNDITGGNSGSPVINGNGELIGLAFDGNWEAMSGN
                     IAYEPKLQRTICVDIRYVLFVIDKYAGAQNLIDELTFGPEKIKVPREKFETTLGKK"
     gene            complement(45408..47105)
                     /locus_tag="PKZ74_00400"
     CDS             complement(45408..47105)
                     /locus_tag="PKZ74_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPM91235.1"
                     /translation="MLNNVFSKILLVVTVIIAVSCQQPQKTEFQMEQQYPGTVTGQQP
                     LAPASPEHVANRLATFAPFTLTTDISHLTANEKQIVAIFFDVADIMDELFWLQAMGPK
                     EPFLARIQDETTKQFARINYGPWDRLDGNNSFIDGIGEKPAGANFYPADMTKEEFEAF
                     SDPNKKSLYTLIRRNADGSLRSVWYHQAYETQLKKAAELIKQAAALAEDAGLKKYLEL
                     RADALLTSDYQPSDFAWMEMRNSALDFVVGPIENYEDGLYGYKAAFESFILVKDPVWS
                     ARLVKYAAMLPDLQRGLPVREEYKTEIPGADADLNAYDVVYYAGDCNAGSKTIAINLP
                     NDEEVQLQKGSRKLQLKNAMQAKFDKIMVPIAELLIDPAQRKHVTFNAFFENTMFHEV
                     AHAIGVKNTINGKGPARTALKETYSSIEEGKADIMGLYLVTKLYEMGELTEGEVMDNY
                     VTFFAGIFRSSRFGAASAHGKANMMRFNFFRSEGAFTRSEEGIYTVDFDKTKAAMEKL
                     VQKIIYIQGDGDYEAAKNWVATEGIVTPALQTDLDRINASGIPVDVVFEKGKQMLGL"
     gene            complement(47198..48289)
                     /locus_tag="PKZ74_00405"
     CDS             complement(47198..48289)
                     /locus_tag="PKZ74_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM91236.1"
                     /translation="MKKALKILAGVIVLSIFVYTIYFLYQKSQAKPVVFETARAFKTE
                     IIKSTVATGSVVPRKEIMIKPQVSGIVEEVYVEAGDMIRKGDLIARIKIIPDMVTLNN
                     AQSRVNRAKIGLEDAKRQFERQGDLLKQGVIAKAEYEPYEIAYKNALEEVEAAENNLE
                     LIKEGQTKKGGQATNTIVRSTAEGMVLDVPVEVGFSVIESNTFNDGTTIASVADMGDM
                     IFEGKIDETEVGKIREGMPLILTIGAIDDASFDAVLEHISPKGIEENGAIQFEIKANV
                     SLKQDQFIRAGYSANAKVVLDRTDSVLAIPESLLLFEGDSAYVEVETAPQMFEKRYIK
                     TGLSDGINIEIKSGLTPEDKIKKPGTSLK"
     assembly_gap    48468..48567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(48617..49888)
                     /locus_tag="PKZ74_00410"
     CDS             complement(48617..49888)
                     /locus_tag="PKZ74_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91237.1"
                     /translation="MSIVDYDKWQEIVIVLKKNKLRTFFTAFGVFWGIFLLVVMMGSG
                     NGLKNSALEGFGNMATNSFFMWTQRTTMPYKGFQRGRWYNFKNADAEAVRLNIPEVDK
                     VAPRLQGGGFRGAGSTVVRGERTGAFSTFGDTPEFFEIMPMNVTMGRVINQNDLEEQR
                     KVIVIGDRVYQEMFKKDENPIGEYLQISGVYFKVIGVVKSKQRGPQAEQDEKAIHMPL
                     NTLQQTYNYGDIIGWFSITAKKGVPASVAEQKTKDYLKTRHHIHPDDNRAIGSWNMEN
                     EFNKFSNLFAGITILVWIVGSGTLFAGVVGVSNIMLIVVKERTKEIGIQRAIGAPPSA
                     IMSQIVIESVFLTTLAGYIGLVVGVGLIELVDFMITKSGAESEMFRNPEINFNMAMLS
                     LGILVISGILAGLIPARRAISIKPIDALRDE"
     gene            complement(50022..51263)
                     /locus_tag="PKZ74_00415"
     CDS             complement(50022..51263)
                     /locus_tag="PKZ74_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91238.1"
                     /translation="MGIFDWDKWQEIFETMKKNRLRTFLTGFSVAWGIFMLIILLGSG
                     NGLENGVKKAFEGDATNTLWINPGQTSTAYKGLKAGRVIQFTNEDYDLISREIPNIYK
                     ISGRVNVWQNNTISYNKEYGNFDIIACHPDYGYYETIDITGGRFINEEDIRDFRKSVV
                     IGKPVKEALFKDEDPIGKYIQVSGVPFKVAGVFSDVGGDRDMQRVYIPLSTAQRVFNL
                     GNRIFNVSVVTKDVTVEESREMAEDVRQTLAQKHKFDPEDQRAVFVWNNLEEYKKLMT
                     LFASIRMFIWIIGIGTIIAGVVGVSNIMMIVVKERTQEIGIRKAMGATPWSIVSLVLM
                     ESILITAFAGYVGLVMGVGLLELISPAFNNSDTFFKNPEVDIFIALGATIVMVVSGML
                     AGFVPARKAAAIQPIEALRYE"
     gene            complement(51339..52049)
                     /locus_tag="PKZ74_00420"
     CDS             complement(51339..52049)
                     /locus_tag="PKZ74_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM91239.1"
                     /translation="MISLRNINKSYGLGKTSLHVLKGIDLDIKKGELVSIMGSSGSGK
                     STLLNVIGILDNHDQGEYILDGLAIRDLTEKKAAQLRNQMIGFVFQSFNLISFKNAME
                     NVALPLYYKGISRKKRNKTAMEMLEKMGLEEWWHHLPNELSGGQKQRIAIARALVSNP
                     RIILADEPTGALDSKTSMEMMDLLKEINQSGITILIVTHEYDIAHSTDRIIHLKDGLI
                     ERTEVNGKNKNQYLDRVN"
     gene            complement(52067..53497)
                     /locus_tag="PKZ74_00425"
     CDS             complement(52067..53497)
                     /locus_tag="PKZ74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM91240.1"
                     /translation="MKKLTAAFLVLFSLAASSQVKEFTLEDCINFALDSNINIKKQVL
                     MVESQKAQLLQDYLVMLPNLNAGATHGYNWGKTIDRYTNEFATKRVQSNNFYLGTQLN
                     LFQGLRQINTVNQAKLDYAAIQYDLDLMMDDISVAVAGYYLDILYSQELLAVSQEQTG
                     ITRQQVGRMQKMVEAGTIARGDLLNLEAQLATEELQVVEAENRLTIAYLSLQQLIDHP
                     YSPDFRIETPKLKPIQAPQIVLTPQNVYDVALSQRPEIKSAELRVRSAEKGISIARGY
                     LSPTISFSGTLATGYSGLEVPTDETINITGVPIGYLQSNPTEIVVSDYTTSAGNFYIP
                     SFRDQFEDNQNTSLQFSLQIPIFNGWQVRNAISQAKIQKEQADLNLAQAKLDLDKKIQ
                     QAYADAVAALKNFNASEKKVNAQKEAFKYAEQKLAVGLVTAVDYNETKKNLAASESEL
                     LQAKYRFIYTTTILDFYMGKPISLVN"
     gene            complement(53807..55873)
                     /locus_tag="PKZ74_00430"
     CDS             complement(53807..55873)
                     /locus_tag="PKZ74_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91241.1"
                     /translation="MKNQTGILLFIILLLAGCTTPTTQPANESTEMKLSIVDEASRSD
                     VVRALKEKYGQNQALRIETGVKQAADFWNGTDGTTDEFKAFCMEKFIGNEAELDLVFN
                     RLSAALESLGGCYNKISVDLKLPLHLPLGDIHAVDEILGGYNPGAHFTDDMFENKLAF
                     IVLLNFPFHNLEEKTDKGEGWTRKEWAYARMGELFTSRVPASLNQNISKTLTDADNYI
                     SGYNICMDKLRDNEGRQLFPDNLRLISHWGIRDELKSHFGEEGGLEKQKLIYQVMLRI
                     INQEIPAVVINNNEVTWNPISNEVFQGDAAADNTAEPGTRYEVLLKNFKALSATDAYQ
                     PFYPNQISRAFDEGMEITQEEVEKLFVELVSSPEVKKAGELISKRLGRPLEPFDIWYN
                     GFKAGTSLNEAELNKITRAKYPDNKAFEKDLPNILVKLGFDKDSAAIIASRITVDPAR
                     GSGHAWGAEMRTDRARLRTRIGEQGMDYKGYNIAMHEFGHNVEQTLSLYNIDHYILHG
                     VPNTAFTEALAFLFQRRDLAVLGIAREDPMENHLMALDNLWANYEIMGVSLVDMRVWK
                     WLYEHPDATVPQLKDAVISIAKDVWNSYYAPVFGVKDTPILAIYSHMIDNPLYLSAYP
                     VGHLIQFQVEQYMEGKKLPDEVYRMYTLGLLAPQVWMQQAVGQPVSIRPVLTAAGDAI
                     KALSNR"
     gene            complement(55913..>56516)
                     /locus_tag="PKZ74_00435"
     CDS             complement(55913..>56516)
                     /locus_tag="PKZ74_00435"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPM91242.1"
                     /translation="METIFLTDSPLFTWVILPLLIFLARILDVSIGTLRLIFVSKGFK
                     FYAPILGFFEVIIWLLAIGQIMQHLDNILCYIAYGLGFATGNYIGIYLDEKLSLGTVL
                     IRIVSKSEITGLVDHLRKEEFGASLVDIEGMTGKQKMIFTIVKRIDLKEVMKIIQEHN
                     PHSFVTIEDVKTAREGYFRYSRRRSLVPVVGLFQDFRKGK"
BASE COUNT        14229 a        13594 c        13348 g        15145 t
ORIGIN      
        1 ctacgataaa ggacaaacct atgtgtcacc cacagttgaa aatgctgtga ataagacata
       61 ccctatctcg cgtccgctct actattatta cctgaacaat gtgtcagcgt cggttaagcc
      121 gttcatcgac tttatcctgt cggctgaagg gcagaaaatt gttaaggaag tgggttatgt
      181 tcctgtgaaa tagtatattt gataataaat tactgggttg agaaagtacc ggaaaatatg
      241 ggaaaaactg gctgaaagcg tgctcttttc aagcagcaca gtcaccagcc tgaccgtcat
      301 cctgatcatt gtttttctct ggagagaagg tgtaggatta ttcaacagca agcctgtcga
      361 taaaggatat gtttttgccc tgaatcaggc gaatccggtt aacaccttga gcgccaaaca
      421 actcaaagat atctttaacc aggatattac aaactggaaa gaggtcggcg gagctaacga
      481 caccatcatc ctcctgacaa ttaacgaaat accaaactat ttcacggaag aggagatggg
      541 caccgatctg gaaaatcttc cggtgctgat cgacaggctc atcctggaaa attccggtgc
      601 aattgtattc atatctgaga actatatgcc ccaggatttc catggccgga tcctggatgt
      661 ggaaaaaatc agtcttaagg agttcacttt tgggacagag tggtatccga cagcagagcc
      721 ggtgcctctg ttcggggtgt ggccattgat cctggggacc ttgctcgtga cgctcggggc
      781 catcgttttt gcccttccat taggcctggc aacggccatt tacctggccg aggtttcttc
      841 tttccggatg aggaatatta tcaaaccact ggtcgagctg cttgccggta tcccatcggt
      901 cgtcttcgga tttttcgggc tggtggtcct ggtacctctg atccagcagg ttttcagcct
      961 ggatgtaggt gaaaccgccc tggccggcag tatcatcctg gggattatgg ctttgcctac
     1021 catcatcact atttcagagg acgccctgcg aaccacgcca aaatcgctga aagaggccag
     1081 cctggctctt ggcgccacac gctggcaaac tatggtgcgt gtaaccatcc cctatgcccg
     1141 ctcagggatt acaacagcca tcattctcgg tattgggcgg gccatcggcg aaaccatggc
     1201 cgtcctgatg gtcacaggaa atgcagccat gatcccaaca tcattcctga tgccggtccg
     1261 taccatcccc gctaccattg ccgccgagtt aggagaatcc ccgtatggcg ggcttcattt
     1321 ccaggcgctc ttcgctttgg gcatcatcct cttcctcttt actctcatta taaacttctt
     1381 catcgaactg gttaaaggcc gtaaacatat taaaacgctc tgatgatggc gatgacaaaa
     1441 aaaaggatca tacagaaaat agctttttca gcattcggag cggtcagttt cattgttgtg
     1501 gccgtgctgt ttataatcct gggttttatt gctttcaaag gagccggcgt cataagctgg
     1561 gaattcctga ctgcaatgcc cgaacaggga atgacccagg ggggaatcct tccggctatc
     1621 atcggaacgg cctgcctgat ggtcggaagt atgatcttcg cctttccggt cggcgtactc
     1681 tccgggatct atatcaatga atatctgaaa gacagctttt tcaagcgttt tgtcaggatg
     1741 atgaccgata acctggccgg aatcccttct attgtctttg gactatttgg tatggcattg
     1801 ttcgttaatt atctcgattt tggagattca atcatagcgg gttcgcttac gctgggttta
     1861 ctggtgctgc ccgttgtcat ccgaacgacg gaagaagccc tgaagtcggt cgacgattct
     1921 ctcagggtcg gtagtcttgc tcttggtgcc accaagctgc aaaccatcca gagagtcgtc
     1981 cttccggtgg catttccgaa catcatcacc ggcctgatcc tctctatcgg gcgggtatcg
     2041 ggtgaaacgg cccctatcct gtttactgtg gcagcgtatt tcctgccccg ccttccaaac
     2101 agcatcttcg accaggtgat ggccctgccc taccacctgt atgtgctcac caccagcgga
     2161 actaatatcg aagaatcacg gccaatggcg tatggcaccg cactggtatt gatattgatc
     2221 gtgttattga tcaatgtcat ggccagcacc ctgcgcaaat acctcggcaa aaaagttaaa
     2281 atgaattagg attattgacc atgtataaag cggaagcaaa gaatgtgaac ttttattacg
     2341 gcgattatct cgctttgaag gatattaacc tctcttttcc tgataaaacc gtaacagcgc
     2401 tcatcggtcc gtccggatgc ggaaaatcga cgtttctccg gctctttaac cggatgaacg
     2461 acctgatcga tcatgtaaaa ctcgaaggcg aagtcctggt gaatggagaa aatatttatt
     2521 cgaaagatat cgtgatcgat gagctgcgga aaagtgtcgg gatggtattc cagaagccga
     2581 atccgttccc gaaatcggta ttcgagaatg tggcgtacgg attacgggtg aacggggtac
     2641 gcgataagaa ctacatcgaa gatgttgtgg tgaactcatt gaaaaaggcg gcgttatggg
     2701 atgaggtaaa ggataaactg aagaaatcgg cctatgaact gtcaggaggc cagcagcaac
     2761 ggctttgcat cgcccgcgcc ctggccgtcg aacccaccct catcctgatg gatgaacctg
     2821 cctccgctct tgatccgatt tcgacagcca aaatcgaaga actgatccat gaactgaagc
     2881 aacaatatac gatcatcatt gttacacata atttacaaca agcaggccgc gtgagcgatt
     2941 ttacgacgtt cttttacctg ggggaactgg ttgaacatga tgtaaccaag aagatgtttt
     3001 taaatcccgg cgatgaaaga acccagaact atatcacggg caggttcggt taatgattgt
     3061 aactctatca attatcttaa aatttattca gatgaatcac ctggaagtcg aattaaaaaa
     3121 cctgaaatcg agcctgctgg agatgtggtc gattgtgatc agccagcacg agaaggcaaa
     3181 gatggccctg gataatcatg ataaggaact cgccaatgag atccatacca atgaaaaact
     3241 gctcgacgcc tttgagttga aacatgatat ggactgcgag aacctcctgg tgctgaataa
     3301 cccggtcgcc gtcgacctcc ggttcgtcct ggccgtactt aaaatcaatt acaacctgga
     3361 acgcattggg gattacgcca attatatcgc caataccatt cgcaaatctg aaaccccttt
     3421 cgacaataag cttcttaagg atttgaacat cgggcagatg ttcgatacgg ctcacctgat
     3481 gatggtcgga gcctatgaag ccttcgataa atgtgaaaca agccacgtga ggcatttttc
     3541 agagttggac aataaactgg atcaatggaa caaggattcg ttcaggatat tgagcgaata
     3601 tatactcaaa tatcctacgg aatcaaaaca agccctggag ctcttctcta tcatccggaa
     3661 actggaaaga gtcggcgacc ataaccagaa tattgccgag gaaatcgttt tttacctcga
     3721 cgccagggtc atcaaacata aaaaattaca ccacaagccc aataattgaa cactgcaatg
     3781 atcaggttca ccaatcctaa ccggcttgca ttgcttatct ctgctgctaa ttctttattt
     3841 atcagcatgg tatatggggt cgggattatg gggaatatga tccctttcag ctggcctttc
     3901 ttttttatta tcaatgcatt gctctttcta ttttcatatt tctttatccg ttatattatc
     3961 cagaatttca tttttcagaa gatcaaagtc atttataaaa tcatccgttc actgaaattg
     4021 tcgcgcacgg aaaaactgga atggcggtca aatcttgggg aggacatgat cgggcaggta
     4081 agccaggatg tgatggactg ggctgagaac aaaactgctg aaatcgaaca gcttaggaat
     4141 atagagaact acaggcggga gttcctggcc aatgtatcgc atgaactgaa aacgcctatt
     4201 tcgaatatgc agggctatat ctccaccctc atctcaggcg gtatcgacga tcccgagatc
     4261 aacagggatt acctgcagaa aacagaaaaa aatatcaacc gtcttatcga actggtcgat
     4321 gacctcgata taatttccag cctggaatca ggtgaagtca acatggataa atcaaagttc
     4381 gatatgaggg ctttaacccg cgaagtcttt gaattcctcg aagacagcgc aaaaagcaga
     4441 ggtataagtt tcttattcgg aaatgatgac aacaacccgg ttttcgtcta tgccgaccgc
     4501 tacaggatca ggcaggtatt ggtcaatctg gtcgataatt ccataaaata cgggcgtgac
     4561 gggggccgca ccaaggtcag tttttatgat atggacgata attacctgat agaggtttcc
     4621 gacaatggaa ttgggatcga agaacagcat atacccaggt tgtttgagcg tttttacagg
     4681 gtcgataagc atcgttcacg cacggaagga ggcaccggac tgggcctggc tatcgttaaa
     4741 cacattctcg aagcccacga tcaaacggtt aacgtcagaa gttccccgaa gatcggctct
     4801 actttttcat ttacactcag gaagggataa aaaaaagagg gtgccgatga gcgacaccct
     4861 cttttctatt attaacgcgc ggactatttg gaagtgaaat tttcaagctc tatttccacg
     4921 gtctctccgg cagtatcaat acaaatttcc ttctcgcatt ccgtataaga gatcagggag
     4981 cattttaaag tgtatttgtc aggcgcaata ccttcaattg aaaaatttcc gtcaaaatcg
     5041 gtgtaaacct caatgtcagt cccctcaacc ttaaccaggg ccccggccag cacttcctgg
     5101 tttactttgt ctacaacctg tcctgtgatt ttaaccgatt tgggggtgag atcgggatta
     5161 ttgtcagcaa aggcggcaat cccaaaggat gataaaacaa cgatcgcgaa aatattaatg
     5221 gcttttttca tggctgtgat tcttgatttt actccgcaaa agtaaatcct caatgttaag
     5281 aaatcattaa gccagtttta ggtatatgta aagtttgagg gggattataa actgttttgt
     5341 taataggatt tttgtgagat gacctttccc ttctcatcgt acatcatcca aacacccgat
     5401 ttttctcctt tgatataggt catatcatat cttaatgtcc cattctcgtc ccagatatac
     5461 cattttccat ccttgaggtt atccgtgtaa ttggcttcgg caattttgac ctcctgctga
     5521 ttccactttt cccatttgcc gtgcatcata ccctctctga acgacctgat ctcctccagt
     5581 tttccattgt taaaataaac cttgctgata ccgcttaata cgccgttttg gatttcaaat
     5641 tcggcatgaa gggctgaatc aggataataa tcgcgggtaa tgcctgtgaa aagcctgcca
     5701 tccttatcat aataaagctt gtcggcaccc tgctgagcct gtccgaaaac tactgaggcc
     5761 atcagcatag cgaaaataga attgatcaag agtttataca tgttttagat atttaaacga
     5821 atttaagatt ttaatgatta agaaaaaatg atgccgcaat attctgtcct gaaaagatca
     5881 gaaaaacgaa taactgaacc gtatctgaaa agtaatcccc tgcgaaagcc ttgaaaaata
     5941 ctgatcggta gccatgtaag aaatgaaaac cgcatctttc caatccgcca gtatattttc
     6001 cgctttaatc cccacctgga atttattgtt tttcccaaag ctcttgttgc tgttgaaatt
     6061 caggctatta aagggaaggg catagatgtc gggacggtca accatgccga catactgaag
     6121 ggttttcccc tggacattat agaatagtcc ggcttcgaaa ccgttccaga accctttttc
     6181 cccaccgtca tacgacaatc ccaggttaat gatatatggc gcctgtccgg ccatatcgcg
     6241 gtactccccg accgtttcac caacccgcgc attatccacc ctcgattgaa attcgacatt
     6301 gctcatccgg ataaatgacc gggtataggt caggtttgag gataaagaga gattcctgag
     6361 ctcctggctg atatttatca ggttgaaccg gaattcgagc tcggcgccga aaacctgccc
     6421 gtcgcccacg tttcgcggct ggaaagagcc ggcctggacg gcgtattgca cgatctcgat
     6481 cgggttgtcg aacatcttgc agaaagcgct gaccgacacc atctgcccgt taggtttgta
     6541 tagttcccag cgaagatcaa tattgtcgat gtctgtcgaa accaggtttc catcccagta
     6601 ttcgatcccc agttgctcgt cggcatcccg gaaaagtccg ccgataaaag tccggccgct
     6661 gatcgggtcg tagatctcgg catacgataa ctctttgaag gaaggacggg cgatggtccg
     6721 tgcatagctg aagcgcaggt tttgctgctc gttcaaagca tagatgaggt tcagggcagg
     6781 gaaaaagcca gtgttatcca gaactttttc attatccggc acgatggtgc cctgctggtt
     6841 ctggccggtg taccgctgaa catagttttc aacccttacg ccgataatgg ctttgagccg
     6901 gttaaaaggg ctgacctccg ctgaaacata accggccatg ttgcgaacac ccgaattaaa
     6961 ccggttggca ttattcggat agaaagccgc ttcataactt gttccgtaat tcaggtttcc
     7021 attatacggc cataggttct ccgggctgaa aatttcatcg ggatcgccgg tcagcggcac
     7081 attccggata ttgatcgcat aattcctgat gataaaatca cgctccttgg aggtgtacgc
     7141 tccgccgaac atgacccggg catttctttt ccacaggtcg aactgccggg tcacgctgac
     7201 aatccccgca tagctgatct cctccaggtc gcgccatatc ctctccggga aaccggtttc
     7261 ggtgccgatg ataaatttac cgttgtcagc tttataccgt gtaaaccgga tatcgggatc
     7321 atacattttg gataatgtcg gcgacagttt ccattcgatt tcccattcag aaccggccag
     7381 gttatgtttc ccgctgatga taaggttggt cagcgagcgc tggctgtatt ccaggttgtg
     7441 ctggaaaccc ccgaagatac tcccctggtc ggagccctgg tattcaaaaa cagcggccct
     7501 ggattcgccg ttctggaggt gcacgatgtt gatcctgtat ttcgaagcct tcgttttaac
     7561 ggcaaatccg gccatgccgc tccacaaaac actgcttact ccgaaattac ctttctggta
     7621 ttcacgcaca tccatctcaa aggtgttgga atcggtcagg ccataacggc cgtattcggc
     7681 gtttttatac atctccgtat tgcttttata ggaaagcgag aagttatatc ccagggtgac
     7741 aatatttccc acatgctgat ttccgtatgt agcgccgata ttgtaatcca taagactggt
     7801 ctgttccctg gcagccaggg tagtattaaa accgcctaaa atctctttgt accgtaatcc
     7861 ggcatccccg ttgggatcaa ggtaagcctc cacaaattgc ggaatatttt cggtggccgg
     7921 aatagcacgg gtgccgtcgt catacccgag ccagtcggtt tttccgcctt cataggtcag
     7981 ataatcgctc cggaaatgaa agccgggatt ataagccaac ccaaaggcaa tactgccttt
     8041 tcgttcctca ggaaagtctt taatggcaat gtcgatcacg ccgccggtaa aatcggccgg
     8101 cagatcggct gaaaaggatt tgtgaacgat gatgttatca atgatattgg taggaaaaat
     8161 gtccatctgg atggtattcc ggtcgggatc cagcccggga atatcgacac cgttcaggat
     8221 tgttttcgta tacctgtcgc ccaggccgcg gacatacaca tatttccctc cttcaaccga
     8281 taccccggtg accctcttca tggaagccgc tgcatccgaa tccccgatct ttttaaatcc
     8341 tgtggctgaa ataccatcga tcacattagc cgattttcgc ttcagggtga gcaaggcatt
     8401 ttcggtattt ctgatcccct gcgccgcaat caccacttcg gtcaattcca cggtcgcctc
     8461 tttcagccgt aagttgtcga ataacgttac atcgccttcc tctacccgga tacctttgat
     8521 gatcatggct tcataggata tgaacgaaac cctcaggtca taatttcccg caggggcgct
     8581 caggttgaat ttcccgtcga gatcggtgag ggtcccgagg gtagtccctt ccagaaaaat
     8641 agtaacaccg ggcaggtatt ccccggtggc atcatcataa acggttccac ggatcgtccc
     8701 gttttggccc tgtaaagagg cggccagaat caggaaaacc aacgctgcga atacttttat
     8761 tgcccgcata gtttgtgttc taatcattat taagtaacag gatattaaat aaacaatctt
     8821 cgtggccctc cgtgccttct tcatgtttct cagcgtaact tatgagtata cgttacacga
     8881 agattcacga aggagtcacg caggatcacg aagtttaaaa caaattgatt aaagggttaa
     8941 gcccacttta ttcttaagtc attaaaatcc attgagcgct cctgaaacgg ccgcccatga
     9001 ccatgtctgg aatttggtaa cgtcggcgcc aacggtattg gctccttcag ccactatggt
     9061 tacgtaagcg tcgctgcccc ctttgaaata atcggtgacc acagcacctc caggcagtgt
     9121 agcctgaaga ttggaacagg tcagtactgc cgggttcagg tcaaaatcct ggccttcggc
     9181 tacaccgaaa aagtaaatac cggtaatggt gcaacgggta ttttcatcca ggtccaccaa
     9241 tccttcggcg tttccggcat aaacggttcc gtttttaatg gtatggccgg cattgtcaag
     9301 cgcaccttcc gggccgtcaa gttccataca ctcgtctccc gggttcacga cgataaaatt
     9361 atcgagcgtg ccggcccagg cctggtcggt atcaaccgcg tcgtcgcccg tattccagat
     9421 gatcgcgttt ttaacggaaa cggttccgcc gaaccactcg atgccgtcat cctggtttga
     9481 tacgatctcg atgttttcga taacggttcc ggagccaacg cctcccaggg taaggccgtt
     9541 gatttcgttg ccttcgccga tgttggcgcc accgtggcgg atggagatgt aacgcagtac
     9601 acccgaattg tcggcagcat tgtcgccacc gtaaaggccg ttctgatccg acggcggaat
     9661 accctcgatc tgcatggatg cggcatcagc ggagataggc gcatttccga ggatcagcaa
     9721 accaccccac aaaccgttga gaaccggatc caggttcggg ctttcgatca tacccggttc
     9781 gatttcatcg gccacggagg tgaaaataat gggtgcatcg gcagtgccct cggccatgat
     9841 cttgcccccg cgggcaatca ggagagcggt ggcgttagcg ccggtacccg cctggccttt
     9901 aacgataaca cccggctcaa tggtcagggt agcccccgaa accacggcaa tacggctttc
     9961 caggatatat actttacccg tttcccaggt ggtgttttca gtgatgtttc cgggaacgga
    10021 gataatcagt tcttcttcaa cgacgtttaa aaccgccgtc gctgaaccgg cctggtcgtc
    10081 attatcggtt aaggtaagta ccacagaacc tgcgcctgca ttggcgccgg cggtgaatgt
    10141 caccacaacg gagcctgatg tgcttccggc agtaccgtcg gtttttaccg aagccgtacc
    10201 gttggttgct gtcacagcag aggatttaaa cccggcttcg gccgtaaaat taaaggtcag
    10261 gtcaatagct gcattgattt caacgtttgc attggcaggg gcagtaacag ccggtgccat
    10321 gttgttttcg tctttatcgc aggaagttaa gaatgcgatt aacgatgaga agatcacaaa
    10381 atagaagaga atttttttca tatcagtatc ggttaaattt tttgcaaaag tcacgatacc
    10441 agattaacac tttgttttac aattgttaag aaattgttat gggtgatgag gttgtattaa
    10501 ggaaagatta ataatatatc attcgcatca aaaattccat acctttgccg ggactaaaac
    10561 cattggatgt tattctgtaa atcggattcg gtgaggtaaa taatcttcgg tgttctgccg
    10621 gagattatat cttccattca aacgggcatt ttcatgccct ctgttctatt tatccatttt
    10681 aaaacatttt acatcatgag taacgaaccg aaatccatac acgaattcga ttttaattta
    10741 atctgcgaat atttttcaag cgttgaacga cagggccccg gaagtcctga aatgaccatt
    10801 aaagcgctga gtttcatcga taacctcacc gctgacagca agatagccga cctcggttgc
    10861 ggaaccggcg gacaaaccat ggtaatggcc cggaatgttc ccggaaagat caccggaatc
    10921 gacctttttc aaaagtttat cgacctgttt aatcaaaatg caggcaacct gaatcttcag
    10981 cacagggtta aaggcatcgt gggatcgatg gacaacctgc cctttcagcc tgaagaactg
    11041 gacctgatct ggtcggaagg cgccatttac aatatcggat ttgaaagagg gatcaacgag
    11101 tggcgcaaat tcctgaaacc cggcggcttc cttgccgttt cagaggcttc gtggtttact
    11161 cccgaacggc ctgccgaaat cgaagaattt tggaaagatg cttatcctga gattgatacg
    11221 atcccaaata aggtcttgca gatgcaaaat gccggctatg tcccggttgc aaccattgtt
    11281 cttcctgaaa actgctggat agaaaatttc tatgccccgt tggttaaggc acgtgaaata
    11341 ttccttcaga agtatgccgg caataaaacc gctgaagaat tcatcgcctc agaactgcat
    11401 gaaacccaat tatattttaa gtacaaagcg ttttacggct acgtatttta tatcgggaag
    11461 aagatctgat gatgatagaa gaggctgcct caatggattg agcagcctct tttcctactc
    11521 tttattagca gaaaagttgt aaataacccc aaagctgaac gtatgggcca ggtcattgtt
    11581 gaagtattcc tggtttctat ccagcatcag gttatgcagt ccatccacag aaccattcaa
    11641 cgcaatctca aattgctttg aaatggacaa caacactccg gcctggaccg acaggttgat
    11701 taaaaagccg gagggccagc ctatggctac agttggtcgc accacaccct tgcaaaatac
    11761 atattgaaag gaaaggggta tcctgatctt atactgcctc tccccggaag atgtgttatc
    11821 tttagcccaa tatatcttgt cggaataaat tagaccggtc ttcaggaaca tatgttcaat
    11881 attctctttt cttcccaggt gaacctgcag tccgtaataa ggagaataat aggcattatg
    11941 gtaagtggcg cccgaaacgg cctcaagttt ccatgaaaca atgaactttg aaatactttt
    12001 tgtgtagttt atacactctg catccttaca tacgatattg tggtagtcaa tagcaatttt
    12061 gaccatgctt ttttgcgtaa tgtcttctat tttatcgatt tcagggaaga tcgcgggggc
    12121 atccgacatg taatacctca tcaaaccatt aaaagtcctg tttttcttca ggtagtaacg
    12181 cccgtcacta ttgacgactt cagtattcct gtcgtcaagt tccagtaaat cctgattatc
    12241 tcttttgatg aagtaccggt gagcatttct tattctgaaa aggtcaattt ctccatcgac
    12301 caaaaactct aaaaaacacc atttaacctc tcctgactta tttttaattt gtttcgaaac
    12361 ataatacttc ccattgatat acctgaaggc agaaatctca tcgggattgt aaacccgggc
    12421 atcatctgat ccgatttctc tgaacaggca gaaatcctgt tcagggccca tattccctgc
    12481 cccaaacagc gtatctgcgt cgttggaaat aatataggct gctttggaac tcccctgtcc
    12541 ataaattgaa cccggaagca ggaagagaag aaaaatcaga ctcgttaaaa ctttcatgct
    12601 ccggtttggt cagaaaagcc aattggtata aatgcgagtg caatataatt aatttaagct
    12661 ctccgcacaa aacaaaataa cagaggctgt atcaaaagta ataaaccaca aaggaacacc
    12721 aaggatttca caaaggaaca caaagtctat tatttgataa tctgatactt agtgcgcctt
    12781 cgtgatttac tttgtgcggc ctttgtggtt aaataatagc cttttgagac aacctcagca
    12841 taaagttcaa acagaaaaga atcctattcc ttcactttga actttgaact atttgatcac
    12901 acccagctct ttgccgactt tggtgaatgc atcaatgcat ttatccaggt ggtggcgttc
    12961 gtgagcggca gatatctgta cacggatacg agccaggcct ttagccacga ccgggtaata
    13021 gaaaccaatg acataaatcc cctcggccag gagcttttcg gccataacct gcgaaagctt
    13081 ggcatcgtag agcatcaccg cgcagatagc ggatttggtc ggtttgatat caaaaccggc
    13141 ggctttcatc ttttccagga aatagtcggt attggcgtgc agcttatcct gtaattcatt
    13201 ggtttcggcc atcatgtcga acatggctat gcctgcgccg acagcggccg ggatcagtga
    13261 gttcgagaaa agatacggac gggagcgctg cctgagcagt tcgatgatct ccctgcgccc
    13321 ggtggtaaaa ccgccgatgc caccgccaaa agcttttccg agtgtcccgg tgatgatatc
    13381 cacctcaccg cgaatattga acaattcggt tacacctctt ccggttttgc ctaccacgcc
    13441 cgctgaatga cattcgtcga ccatgatcag ggcgtcgtat ttcttggcca gctcgttgat
    13501 cttgtccatt ggagccacgt tgccgtccat ggaaaacacc ccgtcggtaa caatgatctt
    13561 gaacctggcg gtttttgagg cttcgatcag tttctcttcc aggtcggcca tgtcggcatt
    13621 attgtaacgg aaccgctggg ctttgcaaag acgaacgcca tcgataatgg aggcatggtt
    13681 gagggaatcg gaaataatag catcctcttc gccgaacaaa ggttcgaaaa ccccgccatt
    13741 ggcatcaaaa caggctgcat aaagaattgt atcctcggtg ccaaaaaact cagcgatctt
    13801 cttttcgaga attttgtgaa tgtcctgggt tccacagatg aaacgtacag acgacatacc
    13861 gaatccatgg gagtcaaggg tttcctttgc tgccttcacc agtttcgggt ggttcgacag
    13921 gcccaggtaa ttgttggcac agaaattcaa tacttcctgg cctccactga ccttgatttc
    13981 agctccctgg ggagtgacaa tgatccgctc ttttttaaac aaaccggctt cttcaatgct
    14041 tttcagttca ttcttcagcc aatcctgcaa ttttccgtac ataatcctat attttaaagt
    14101 tttagtgtta aagaattaac aaagtggtgc aaagataatt ttttttaata tcagaaagat
    14161 tcctctccgc cttccggcgg atcggaatga cgaataacaa gcatttgaaa aggagggtat
    14221 tcgtcatccg gagcgaagca aaggatctat aaatctcaaa atgaattatt taacatttca
    14281 ttaacacata tgcaacatat tgcataatat ttttggtatc ccaataaata ttcaccatta
    14341 tgaaaaagct gctaaaaatc tttctttggt tcgtccttgt tattgtcatt atcattgtca
    14401 ttgccgtggg ttacatccag ctcacatgga ataaaacctt tgatgccccc taccccgaca
    14461 tcacagccag taacgacccg gcgatgatcg aaaggggtaa atacctggcc tatggctggt
    14521 cgcactgcgc cgcctgccat gtgccggccg ataaagtcag ggaggtggaa aacggcctcg
    14581 agatgccgct catcggcgga tgggatgagg aaatagccgg ttttggcact ttccgcgccc
    14641 ccaatctcac ccccgatccg gagacaggga tcggcaggat gaccgatggc gaactggccc
    14701 gggccatccg ctactgcgta aaacacgacg gtaaacttct tcctcctttc atggttttcc
    14761 agggattgag cgacgaagac ctgacggctg tcatctcttt ccttcgctca caggaaccgg
    14821 ttaaaaatca agttgcacct tccgattatg ggttcgtcgc caaagctgtc attgcttttg
    14881 gtttgcttaa accacagggg ccgaaatcca ccccgccggc atccatccgt ccggatacaa
    14941 ccgctgttta tggagagtac ctggcaacga gcgtcggcaa ctgcctcggg tgccacataa
    15001 agatcgacca ggccgggaac caggtgaacc ccgatttcgc cggcggcgga atttttcctc
    15061 ctaccgcttt ttctaaaggt tacggatttg tttcaccgaa cctcacaccc gacaaaaaaa
    15121 ccggtgctat tgccgattgg agcgaagagc agtttgtaaa ccgtttccag ggcggccgcc
    15181 tgcacgaggg gagcccgatg ccctggaaca tgtattcccg gatgtcggtc aacgatatga
    15241 aagccatcta ccggttcctt cacacactgg atcccgtcgg gttttcagtg gaaaagaccg
    15301 tatatcagcc gggagagaaa atgcctcagt aggttgcctg tctttcgccc atgacccgct
    15361 tgaaatcgga tgcgaggtta cgggctaccg gcaccggctc ttcaaaacct gccagggtca
    15421 gcttcaatcc ctgcgaattg cccgaaactt tttcaatctt atcgaggttt acgatccatg
    15481 acctgtggca tttgtaagca gccgggatag gcgctatcag atcttcggca tatttcatcg
    15541 tgtttctgat cagggttctg accattttcc ctttttttat gtatccggtg gaaatataat
    15601 tcccttccga ttttataaag accaggtcat caaataaaat ctccagttct tctttgcccc
    15661 cttcggagaa tatcttcagt gcagagaatt gggtttccac gtcgggccgg ggatgcaaac
    15721 cggatgtgat cattccggcg ctgaccgcat tcttccggtt aagatagctc tgtttgatca
    15781 tgacgatcgc aatcaccggg atgatcccga tggcaaccgt aaatagctgg aatatcagaa
    15841 tattctggaa agtaagcctg aagccgatat aaaagtgaga atagaccagg tttcccagcc
    15901 cgatggtaaa gaggataagc aggaagttca gcatttcttt aaagaccgtc catttttcct
    15961 ccgagtaaac tttgggaaat accaccggaa ttaaaatcat gtcgatcatg agaatgataa
    16021 aggtcaccag gccatatccg gccaggatca gcaatttgtc gggcgactgg aactcactca
    16081 gcccgaatgg ctgaaaaacg ggcataaaga acagaatgaa aatggagatc ggaaggatga
    16141 tcttccattt gttctcctcg tatggatatt gctgcaaaag atatttccgg atcattgatt
    16201 tttgtatgtt tgaaaaagaa tgacaaagta aaattaaatg ttttcacaat aagatgttgt
    16261 tgatccgtct ctccggccat tcctgcgcgg gcaaaaccag gctggtcaac gccctgccga
    16321 aatatggcat cacgctcccc aaagtgttgc gctatacctc acgggcgccc cggcccgaag
    16381 aaattgaagg tgaagattat ttcttccgta gccgagaata catcgaagcg ctgcccggca
    16441 aagattttct gaaaggaccg gtaaggaata tgctccaggc cttcgacctg aatgccatcg
    16501 caaatcttct cggcaatcat acaggggtca ttattgagat ctatcccggt ttatggccgg
    16561 aactggtcag ccggctccgg attcggctcg ggaacaacct gaagaccgca tcggtattca
    16621 tgacggctgt cgatccggaa cggatcaggc agtttaaaaa gaaagacgaa atagcaggat
    16681 ttatatcgtc agaagtttat aaaatgcttc ttttcaggaa caaagatgag atcgaagaca
    16741 ttaggatccg tgcttattcg gcggctctgg aaattaccgg ggccctgagc ccgcaaggca
    16801 gaaaagagta tgaccgaatc atcttttctg ccccggaagg gcctgatggt gaagatgaat
    16861 ggacccgcgg accggaacct gtagggcagg ctttaatagc actgaataaa ttcagagaat
    16921 tcctgtatga gtttgaaaaa ccttattaga atactacttc agccccagtt tttcaatcct
    16981 gtcccctacc aggatctgct tcatttcata ttccagtgcc gggaaatccg cctttaaaac
    17041 ccgccggatg atcgctccct ctttcatcag gaagatttcg tcgcaggttt cacttaatgt
    17101 ggagaaaata tgcgaggata taatgacggt ttttcccagt tctttcatcc tgaggataat
    17161 atcggcaatc aggatattgc tctgaatatc aacaccgttg aatggttcgt cgagaataaa
    17221 tacctcattt tcctgcatca ggatcgccag cagcgccagc ttcttcttca tccccgtgga
    17281 ataggtcgaa gcatattgat tcaacgggag atcgaatata tttttttcat gaatgttttc
    17341 cgcctttatt cgcctggcat tgcacaataa ccggatgtat tccatcccgg tgattttctg
    17401 gaagaaatag ggttccgtag ggagatagcc caggtggtct ttaagatggc cgtaatccgt
    17461 aataatctca ccatcatgct tttccaatcc ggcaatacat ttgaacaacg tggttttccc
    17521 cgcaccgttc tcccctacca atccgtaaac acaacccttt tcgaatacca ttcgggcatt
    17581 ttttacgaca tgatgatcgc cataatactt atttaaattc tttatctcta tcatgcaaga
    17641 agggttttaa gccggatcga cgacttcctg tagaaaaacg gaatgacggc gagaagcaaa
    17701 ggcggaaacc agaccgatac gaccaggatg atgaactgcg gtaaattcat cgggtgcgga
    17761 tatgacgaat atttagccag gaccacggcg ccaaggtaaa tatagccaag aagctggaat
    17821 gctccgatta tcaatatctt atcggaataa aagaccgaca ggaccaggct taccggaagg
    17881 gtcaggaatg tcgcgtaaac cagggctgtt tttattttcc cgaacaggaa aataccggga
    17941 cgactattga aaatccagac atagaattca ttttcaggat tgaggtaata gctgaaacaa
    18001 acaaggaaaa ccaatatcag cgatgcaatg ccaaggttga agttgccgga gatcaccgcc
    18061 atgaccgtaa gaaaccaggc caggacaatc accggaaatg agttccggaa tcccacggta
    18121 aattcaaatg gccgcttccc gaagggggtt ggaatggtaa agccggaccg gttgccaaca
    18181 atggtcaacg acataaaacc ggcaaggaac aacgcggcaa gggcatgaaa ataatatttt
    18241 tcgattacga gaaaagcggc aaacggagcg gcgacgatga cattttcaag catcctgacc
    18301 acccggtaat cctttttccc gaaacagctt ctcaggaact ggttccgggc cggatcgctt
    18361 aatctggcaa gaaaactgag gcttgcggcc aggtaaagat attcggcgaa agctgttttt
    18421 tcaaagatga ggaccgatga gccagtaaaa gctgccagga tcagcacgca gccaataacg
    18481 ggatggatcc cgaaagccgc caggtgcctg ttgagcattt taaactgtat cagaaaataa
    18541 agtttcattt tgagccggga attacgattt catcggtgaa taccattcct gtctcctccg
    18601 ccggcagttc catcagcctt gccatttctt tgataatgtg ttcattatcc atcatattct
    18661 cccgatccaa caaatgtacg gtcagttgtt gccgtaagtg aaacagcttc cgcctggtat
    18721 ccggatcaaa catcccccgg ttgaaaaaat tgctctcttc cgaatagttg tacgggacat
    18781 tggtaactga ccggaagact tcccggcagg cgttgtccgc ctgttccgaa ttcatcgaat
    18841 aatgcgacca ttgatccata aatccttcga tcttccattt tttccggatc gcaggttctg
    18901 ccagttcgct taggttgaat atcagcaagg ggtaaacctg gtagctgacg cccaggtgag
    18961 tcagggccag gttattcatg aaatcgatgg tattgcgcaa cgtctcttcc gtttcgcccg
    19021 ggaagccgac gacaaaggtc atcagtaccc ctatcccctg cgcatcgaac tgctcgatcc
    19081 ctcttttcac cctgtcaatt ttctgctttt tgttcatccg gtcgagctga ccctggtcgc
    19141 ccgattcaac ccccatcttt cccatcatca gtcccgaatc gaggatcgct ttcatctcat
    19201 cttccgaata ttcccctccg cgcataaaac ccgcccagtg gttaagtccc gattttgaaa
    19261 gtgttccgca gatttcgaac aatcttttcc gggtgatgaa cacattatcg tcggtcacat
    19321 ggatcatggc gggagtgctc ccggtccggg atttgaccat gctcagctcc ttgcagaggc
    19381 tctccggaga gcgcaggaat attttcggga aaaggctgca gaaatcacag aaccggcaac
    19441 ggtatgggca tccgatcgat gtccggagcg gtattttttc cggcatctcc gaaatcaaat
    19501 cccatcttgt gaaatcttca tcgtaatcga ccttttcttc caccatgccg gtaaaaacaa
    19561 agccatcctc ccctggcaag cacaaattcg gaacatttga taatcctcca atcccccaat
    19621 cctcaaatcc cttacctccc ttaccacctt taaaaccctt aaaaccctca ctctcaatca
    19681 cctccagcac cctcatcaac gatccgatcc catgctgcgc cacgaccagg acatccgctt
    19741 ttattgcgga attggaagga tgaaacagca tctcgctgtg caaaggaaaa tcttccggcg
    19801 acgacaggtg acgttcatac tggaaataat tcttccagat gttaaccccg ccggcgatga
    19861 ccgggacgcc gggcatggct ctcttaaccg acgcacacaa cgctaccagc gaatcggtcg
    19921 tgatgatcat cgtcgtcgaa atacagacgg cgaagatctc tttccgggcc agcgcttcca
    19981 atgttaaatc atcatatttg ttggtcagga tggtctcata gccatgggca tggagtatcc
    20041 cctggaggta aaacccggcg gccgggatat cttcgccgta atcgataccg cggggcagat
    20101 caacgccgga aaatccattg ccggcccagt tgtaaaacgt cgccagggta agttccctgt
    20161 tatctctcgc cagccgcagg gtaaaatcca tattgaactg gcgggtatcg atgaacgggg
    20221 cgatgatggc tatggtccgg ggcatggaaa gtactttgtt ggatttagta agtgtttagt
    20281 gattaggtgt ttgacatttg taaattaaat agcaagaagg gttgcaagat caaatttcct
    20341 cattttcagg aaagcattga tcactttctg tgatttttcc ggatctgcca ttaatgcggg
    20401 aagaattgaa ggtacgattt gccacgaaac accgtatttg tctttcagcc atccgcacat
    20461 gctctcttct ccgccatcgc cggtcagctt attccagtag tgatcaattt catcctgtgt
    20521 atcacaatcc accgcaatgg atactccttc actgaaccgg aaagggtgat caaaagagct
    20581 gtccatggcc atcataccaa aaccatccaa agtgaattgg gcatgtttaa cttttccttc
    20641 ttcatcatca tcattggccg aatattttaa aagccctccg acagaagagt ttgggaaaag
    20701 agctgtgtaa aaacggatgg cttcttctgc ctttccaaac tgctgatgcg taaacatgag
    20761 ggtaggacag atcttttgcc cggtatcttt cggattgcca agatacaact gccagcttat
    20821 cccaaagcgg tcctgcaccc acccgtaacg ccggctccag ggataggcat ccagcggcat
    20881 cataggcttt ccctccacga tcagcttctc ccaggcatga tctacctcct cagttgtctt
    20941 acaggtaacc atgaatgaaa tcgacggatt tggctggtac atcggtcctc cattgagcaa
    21001 catgaacttc tggccgaaaa tttcaagagt aatgacgatc ggattttctt ccagaatgac
    21061 cacatcttca aaagcttcgc gataaagaac cgcagcttta ttcgctttgc tgtcatacca
    21121 taaacaagga taaattttat tattcatttt gggattaaat tagtgggttg aattttttac
    21181 aaatttaata ctgccaggca accataagac ctgtttttaa cactaatcat gtaaataccc
    21241 ggaaatggac tttctgactg aagaagaaat aattgcagga tgcctgaaaa atgacaggaa
    21301 agctcagaaa gccctttacg atcaatacaa atccaggatg tttacactcg cttacaggat
    21361 cacgaacagt tttgaagacg ccggggatgt tctccaggat ggatttatct atgtttttga
    21421 aaacctggat tcattccggg gaaattccag gctgtcgacc tggatccata cgatcatggc
    21481 ccggactgcc cttaaaaaaa tccgcgaaag gattgtcttt agcgacctta cggaaaatga
    21541 gggacctgaa actatcgact ggggcgggga aattgatgtc cagttgcttg aacaggctat
    21601 ccatggtctg cctgacggtt atcgttccgt ttttacactt tacgaaatcg aaggttttaa
    21661 acatactgag atagccgaaa tgatgggaat atcggtcaat acatcgaaaa cacagttgtt
    21721 taaagccaag cggatgctaa ttcagaaact tcgtggcaat tagatggaaa agaaccacga
    21781 catattgaag aaagcgctgg gccggatgaa ggtttacgat cctcccgaag aggtttggaa
    21841 tgcttttgta aagattacgg gagaagggga tagagccctg ggttttcgta aactgaccgt
    21901 ctattctccg cccgattctg tctgggatca tatcgcccgc cggcttgcct taaaagatcg
    21961 gggtaaaggg gaaaaacgga gtattgccat cctgaaatgg accgctgcgg tcgcggcaat
    22021 tgtcattcct ttcgttatca ttttcagtgt gacgggcgac agggaaatca ggacccatta
    22081 ttcagaggaa tgggtcgtga tggccgatat ccgcaataac acaagtgatg attccctgtc
    22141 ttaccacgtc attgacaata aatgccggaa aaagcccgaa atctgccagt ctgatgaatt
    22201 tctcggctta aaaaaggagc ttgcttttct tgataattca aagaaaacca ttataaaccg
    22261 cctgaataaa tatgaatcgg gcaaagatct cgaagccctg ctgatccgta tcgacctgga
    22321 acgggaagaa atcctgggca aattgatcgc tatttccaat tgagatgaaa cgaggaattc
    22381 tatacatgct ctttttcttc atcctggctg gaaactcatt tccgcaagtc agggtgcagg
    22441 ttgtttccag ggaaatcaat aaaacgatcg cctggaaaga ggggatgagt ctggtcgtca
    22501 atggggataa tgctgaaata tactgctctt caggctttca tcaggatatc gggatcagca
    22561 tgactattat ttccaaacat gaagaacgga cagtcgcaga agccgacctt gaaaagatga
    22621 aatggctggt tgaagtgaaa gacaacacct gctttgtcag aaattacatc gaactctcgc
    22681 ggaaccaggc ccggcccgag tcggccttga aagtcatcct ccggatcacc gtcccggccg
    22741 attgcccggt cagtctccat aactatttcg gaaagaccga aatcagcggt ttaaattcag
    22801 aagtgaccct aaacaacgaa tacgggccgg tgcttttgtc agacctgaat gggaatacta
    22861 cggtgaattc ggtctttgga gatgttacca taaatggctc catgggccat acccgaatta
    22921 cttccaccca ttcacagatc gttctccgtc tgaatgctct ttatcaatac ggctttaact
    22981 tcgaattaac ggattctgag attatcaaac cagaagaaat gatcatcatt tatgataaaa
    23041 aagagaaagg aaagatcacg ggacaaatca aggggcaaaa ttccggcccc gaaatatctg
    23101 ttatcctgaa aaactgctcc ttaacaatcg aacaatcgaa caattgaaca atcgaacaat
    23161 tgaacaatcg aacaatcgaa caatcgaaca attgaacaat cgaacaatcg aacacaataa
    23221 caatttaaca atttaacaat ttaacaatgc cgtcatttca ccgaaataag cctgtccttc
    23281 ccctgtacct gatcattatt ctcctgtcac tcctggggtt gaaaccgggt aagactttcg
    23341 cccaggaaga aaatgacagc ccagtaaatt atattaattc catggaatac gctttcatga
    23401 tgcatgaaga tacccggtgg ctgttaaagg ccgacctggt ggtgaaaaat gaatatgtga
    23461 acatgaatcc gttcaaaata tcatttgaac acaggattta taaaagtttg acggctaatg
    23521 cttccattga tcatgtcaac atgaacatgc ctggcgatca tatgtatttt ctgccgttga
    23581 atttttccat tgaaccccgc tggtattacc ggttgagcaa gcggatgaaa gatggaaatg
    23641 cggcctccag cctgtcggac aactatctcg ccatgggtgt gtcatacacc tggttgctga
    23701 attataacca ggtggaatcg ccggtcgacc gccggtatgt ttcgtactac gccaaatggg
    23761 ggatgcagcg ccgttacctg aagtacgggc atatcgacat gggcatcaaa gcggggatga
    23821 tggatgcagc gggcaacggc ttctcccctt ccctggttct gaatactttt gtggagatgg
    23881 ggctgggctt ttcgaaagac aggcaggcaa tggacaggga aaggctgtgc ccgttcctga
    23941 gatgctatga agccgaccgg ttcattgtca aaacgaacct gaccgacctg gtcaatctcg
    24001 gcattttcat ggatcaaaaa tgggtggaac tttctccgca ggttgcattg gagcacaagc
    24061 tcggaacatc gtcattttca ctgaatgcct caatcggtgg gactttgggc tatgctgagt
    24121 tcagtgaggc ggacaaagta ttgaccgacc ggtactggat tggcgaagta agcttagagg
    24181 gaagatatta ctataacctg aagaaaagca tcctcaacgg cctgtcgggt aacgggttgt
    24241 cggccaacta cctggctgca ggaggaggtt attgcgtgac cgatggccag tatgatgaaa
    24301 tgatcaaaga aaccggcccc cggctttacc tggcaacagg gtggcaacgg ctgctcaacc
    24361 ggcatttgta cttcgatatc caggtcgggg ccaattacaa tttcaggcca aaccgcgcgg
    24421 gaaatgttta tgagcccatg ttccaatacg gcctggggta caggttctag catgaactga
    24481 tcggtcttat cgcatataca ccggtttatt gtcacaggaa tcgctggtag aacaacacat
    24541 ccagcacccg gtggaacttc tccccgatct gtttcaggtg gccgcacttt tcatatccgg
    24601 ctctttcaaa caactttata ctcgctatat tatctccgga aatgatggca agcaaaacgt
    24661 gtatcccttt ctcccgggca atttcttcca ggtaatccaa tgctattctc ccgattcctc
    24721 ttcccgtata tccgtttttc aaataaagtg agatttcggc ggtcctgtcg taagcctgcc
    24781 tcttcttata ctgcgaaagg taacagaatc cgcagcattc cccaccggct tcgatcatgt
    24841 acgatttata tctctggcta ttcagtggaa tgaactgttt cagctcatcg atcgaaatca
    24901 gttctgtgta ataggtcgag gtcgaattca gaatataata atcatagatt ccctttatga
    24961 ccgcaaaatc ggcttctgtt atttcggtga atcggatatt cataaccggg atcatcgttt
    25021 aaagcaataa atttaattca aattaccaca tatcacaatt ttcttacttt tgccgcaaat
    25081 tgttaattat ttcacaaaaa ctagatcaaa atgaaaaaca tactcgttat cggctctgtg
    25141 ggtcagatcg gatcggaact gaccatgcgt ttaagggaag tttatggcgc ttcgcatgtc
    25201 gtcgccggct atcgtaaaac catgcccccc aaagaattag cagaatccgg cccgctggaa
    25261 atggtggatg cgaccgacgc tgcgaaaatg gccgaagtcg tgaaaaaata taagatcgat
    25321 actatttaca accttgccgc cctcttatcc gctgtagcgg aaggcaaccc taccatggca
    25381 tggaatgtgg gtgtcggcgg tgtatttaac tgcctcgaag tggcccgtga aaacaactgc
    25441 gccatcttct tcccgagctc catcggcgct ttcggcccct ccaccccgca ggataacacc
    25501 ccgcaggata ccatccagcg ccccagtacc atgtacggtg tgacgaaagt gaccggcgag
    25561 ctgctcagcg actattactt caagaaattc ggcgtcgatg cccgcggcct ccgctatccc
    25621 ggcattatct ccaacgtaac cctccccggc ggcggaacta ccgactacgc cgttgagatc
    25681 tattacgctg ccatcaagga gaagaaattc acctgcccgc ttccggccgg caccttcctc
    25741 gacatgatgt acatgcccga tgcgcttgat gcagctattg acctgatgga agccgatccg
    25801 tcaaagctga agcaccgcaa tgcgttcaac gtcagcgcta tgagcttcga tccggagatc
    25861 atcgccggcg agatcaaaaa gcatattccc gatttcgtga tgaactataa tgttgatccg
    25921 atgaagaaag ctatcgccga ttcatggccg aacagcatgg acgacagctg cgcccgcgcc
    25981 gagtggggct ggaacccgaa atggaagctc gacaacatga cggtcgatat gctgaaggtg
    26041 attggggaaa aacacgcgaa agggttgatc tgacaacttc tttaagttcc ttcgctctgt
    26101 tcgggatgat acgtcatccc gaacgaagtg agggatctcc ctgattttcc ctacctttgc
    26161 cccatgcaaa tcgccgccct cgtttccggt ggagtcgaca gctccgtgat cgttcctttg
    26221 cttaaagagc agggctatga tccggttatt ttctatatcc agatcgggat ggagaatgag
    26281 ccgggattta tggactgccc gtcggaagaa gacattgaga tcactacgtt tatcgcaaaa
    26341 aaatacggct gccgcttcga aatcgccaac ctgcacgaag agtactggaa ccgggtggtg
    26401 aattacacga tcgagtctgt cagcaagggg ctgacgccga acccggatgt gatgtgcaac
    26461 cgcttcatca agttcggcag cttcgaggat aaattcggga aggactttga taaaatcgcg
    26521 acgggacact atgcacagga tttggaaaaa gagcggcagt ttttcctcgg gacctccaaa
    26581 gaccggcgga aagaccagac ctatttcctg gggagaataa cctatcctca gctttcaaaa
    26641 gccatgttcc cggtaggagg gttcgaaaaa gaggaggtga gggcgatggc tgaaaggctg
    26701 aaactgccca gcgccggccg gcccgacagc caggggatat gcttcctggg aaaggtcaac
    26761 tataacgaat tcatccggcg ttataccggt gaaaaagaag gggatatcgt ggagctggag
    26821 accgggaaaa tcctcggaaa acacaaaggc tactggtttc ataccatcgg ccagcgaaaa
    26881 ggactggggc ttagccaggg accgtggttc gtcgttaaaa aggatatccc tgccaatatc
    26941 attttcgtct caaatggcta cgatcctgtt tcgcagcata aacatgaaat ccttctggat
    27001 gatttccttt ttatcaatga aacgcccgac cgcgattatt ctgttcatca gcctgtcttc
    27061 ttcaaaatcc gccaccagcc ggatttcaac gaaggaacga tgaggaggat tgggggattg
    27121 ggggatttgg agattggggg attagaggaa ggggagaggt atgttgttca atcggtgaaa
    27181 agcattgcgg gagttgcgcc ggggcagttc ggggttattt atgatgtgga aaagaaaata
    27241 tgccttggaa gcggagtaat cgccgaaagg gagtgatcga tattgtgata ttatgatttt
    27301 atgagcttgt gattttgtga tatcatgata ttatgaattt gggatattgt tattttgtga
    27361 actaatgaac ttgtgaacta ttgatattgt gatttataat cttatgatat catgaattta
    27421 ttatctgaac tctggaattt gggtcaattg aataatatgg caatggaaca attgaacaat
    27481 tgagccaaac aacagtttag caaatcaaaa ccaactgtca tggaaaaatt cattatgtac
    27541 aatcctgtta agcttcattt tggcagggga gtggtgagtg atctgggaga ctccatctca
    27601 aaatacggaa aaaaagtgct gctggtttat ggcggaggct cgatcaggaa aaatggcatt
    27661 tacgaagaaa ttatttcgca gcttggcaaa gcaggcgctg aagtatttga atacgccggc
    27721 atccggccta accctgttat tgaagatgtg gatgcggcgg caagcctggg cagagagaag
    27781 ggcgtcgatg tggtgttagc cgtgggagga ggcagcgtta tcgattctgc caagattatt
    27841 tccatcacca ttcccgtttt acacagtggc tgggatttta tcaagggaaa gtcaaaaccg
    27901 gtctgtgcca taccgctatt cgctgtactc acgctggctg ccaccggcac cgagatgaac
    27961 cgtttcgccg tcgttcagaa caacctgact aaagaaaagc tgggttatgg cgacctgctg
    28021 acctatccga aggaatcgtt cctggatccg gcttacacgc tcagtgtgcc atccgattac
    28081 acatccttcg ggataacgga cctgatagcg cactgcctgg aggcctattt cggagaaggg
    28141 gaggccacgc tttccgaccg gtttgttttt tcgattatcg gggaagcgat ggaatacggc
    28201 cctcaattga tgcacgacac agggaactac ctgctgcgtg aaaagatcat gtacgcagcc
    28261 acctgcgctt taaataacct gacagtttac gggaggaaaa acggggactg gggggtgcat
    28321 tccatcgggc atgtgttgtc ggttttatat gatgttccgc acggggcatc tttatctatg
    28381 gtttacccgg catggatgag actccatctc gacagggccc cggaaaggat cgcccacctg
    28441 gggatgaatc ttttcgggac tactactgca gaagagacca ttgaaggatt cgaaagattt
    28501 ttcaccggga tcggatcacc tgttcgatta agccagtacg gactggatac tcctgaaaac
    28561 cgcgcggaga ttttccggac gcttaaagcc aaccgggcag gcggaaatca tttcaggctt
    28621 tcggatcagg actatgagaa tctgatccaa ctgatggcct gatagcctgc tattcgggga
    28681 gtgccggaat tttcagcgta aaagtagtgc cggtgcccgg ggtggactca aaagttatgg
    28741 aaccgttcat ggtgttcagg atacttctga ccatggccag ccccagcccc ataccactgg
    28801 atttcgttgt aaagctgggc gaaaacattt tcttagcctg ttcatctgtt atgccgcagc
    28861 cgttatcggt gatcgcaaca atataattgc tcccttcctc cgacaatgag atatcgatct
    28921 ggccctcagg tccgggccct attgcctgta ccgcgttctg tatcagatta ttgaatgccc
    28981 tcaagagctg cttgtcgtcg cccttgacaa aatatgcggg tttttgctgc cggatcgaaa
    29041 tccggatatt ctgatggtga aagtataatt cagccgctga acggattacg ccggtgagct
    29101 caattttttc catgacaggc gccggcattt tagcaaaatc cgagaattcc gatgcgattt
    29161 ccgaaagggt atctatttgt tcaacgatgg ttcgggtaaa ccggtggagg tgcttttccc
    29221 agtcgggatc ctttctttcc caggcttttt gcaggtactg aacgctcaac ttcatcgggg
    29281 tgagcggatt tttgatctcg tgggctacct gttttgccat ttcacgccag gcgctttccc
    29341 gctccgatct tgccagttta tcggcactca gggccagttc atcgatcatc cggttatact
    29401 cctccaccaa atcgccgatc tcatcccttc tttttaggta cagtttttcg tttgattttc
    29461 ccaatcctat cctggccatt ttttctctga tcatttgcaa aggccttgtg ataaacctcg
    29521 acacgagaat ggtgaccatg atggcgacaa caataagaag gacataaaca tttatgtaag
    29581 ccgcgaggaa atcaccaatt tctttttgca gctcggtttg cttggcaaaa taggggaggt
    29641 tgacaaaagc gataatatca tcatctgtat tccgaaaggg gatatatgcg gaaagatatt
    29701 cctgtcggcc gattttttca ttctggataa agaggaggct ttgccctgat gtcatcctga
    29761 aataggccag gctgttcatt ttatccgaca gcagttcttc atcaaagatc tcgggacgcg
    29821 atgaagcgat cagtttgccg tttacatcat acagattgat atcggaataa aaaatcatgg
    29881 aaaacttatg caatatttcg gcgatatatt ctttcaggtc ttctgtaatg gctttttcag
    29941 atccaatctt atgttccagc tctgtcagta ccgaaaatgc tttttcgctc aggttatccc
    30001 tgtttttatc gttatacagg tgaatgatat tggcccgggt gatcataccc aggaccagga
    30061 aggaaacgag gattatggat atgattgcca gttgaagccg gttgctgaaa ctgaactcga
    30121 tttgtgatat ccctttaaat agggtatatc ctcctaagaa caagaggatg gccaggctaa
    30181 acaggataaa aaggtaagaa aatggggcga gtaggtcgag aagggtcagg ttctttttgc
    30241 tgataatcaa tatattatta ttggcggaac gagctatata gtgattatat ccttcattat
    30301 caataaaata atcattactt tccggaatat cataaacatc aaaacccagc ggataaacaa
    30361 actcaccatt cttatatatt aacctgtcgt tgatgtattt agcataggaa tagccggatt
    30421 tatcacctgt cattccagcc gtaccgtcga tcagcaaatc aggatatccc aggccgcttt
    30481 cccgggcata tttgtagaac agttccgcaa acaccgtcag ggagtcggtc ctgttctttg
    30541 taccatacgg taatttaaat atcccgagat agttgtaatt ggtcatccgg tcgtcgagaa
    30601 aatagagccc ggatcccaca ttttcggcga agggagaagt gattcttgaa ttgaaatagt
    30661 catagcaatt ggctatgtaa tctcccggct gtatgttgag tatttcatct tttttgcata
    30721 ttgtgagcac aagttcgtac ctgacccaga aacgggtaaa ataatgatcc tgtagatggc
    30781 cggtcaccag gctgtcgatc ttttccgccc atacatcctt gcttatcagg tcgatcagta
    30841 cgctgtcttt caaaatatcc gtcctgatcc gtgaaaattc gaattccgtc agcggatcgc
    30901 ggcgggatgc cagttttgag gccaggattt tcctgttctc tttttctttc tcagaatagt
    30961 aataatcgag gatgaaggta gcggtaaggg aaaacaatac aataaagacg agggcgccgg
    31021 tcatattttt aaatccacgt ttacggtgtg aaaagtaaat aaatgccagc agataaaggc
    31081 agaaaattac aatgaacacc aatgccgcga tatgatcgag agactggcta aacagcaggg
    31141 ttatcagaat aacggtgatg gtactgatca ggaataatga tcctgttttc ataccgcttt
    31201 tacaaaggag gctaaaaagt tttgcggacg caaagaagca ggacaacacg gcgatggaca
    31261 gggccaggaa tcccagtccg gaatataaat cagcttcaaa tatgttattc aggttcagtt
    31321 caatatttga atcgagcacc agatgtttaa gggccataat gagcagaaag aataacaggg
    31381 cattgatcag catccaaaat aaacccatgg caatcttcaa tatttccgat ttgggaatcg
    31441 ggatattcaa gtcggtgcga taaaataacc atgccagggc cagccagaga atattattga
    31501 tcaccaggtc gcccagtgaa ggagagaaga aggaggtggc gtagtatagt gggctgaaga
    31561 gggctgtctg ataaaggaat cccgggattt cgaaccagat catcgccagc cggagaacga
    31621 cagcatccgc acaaaaggcg ataaacatta tcaccggata gcgtcggaag aaatcaaaga
    31681 ggtggtacag gtaaaataaa accagtacca tgcaaataaa ggcgataata gccaggccga
    31741 aaacggccag gcctatccct tcattggttt gccacgtatc cgggggaatg agggaaaaga
    31801 gaaaatctcc atcctccgaa aagatgttca ggtctctttt cagggtgtcc agtccggcat
    31861 cgggatcaag ttgaaaatct tcatgaaact ggttgatcag gtactggttt tggtaagggt
    31921 aatcggtccg gataaggatc agcacctgtg ctttcaacga accggcagtt ttatccatga
    31981 ccctgtacca tccgttaccg tgatgtaaga tcggtctggc caatccattt ctttcaatgc
    32041 gtcccggggc cggaattgaa ttgtcggacc aaaatataag ggaatcgttt ctatacagca
    32101 gtgctgtcag tccgttttcc tgccaattaa aacttttttc gtttttccaa aactttagaa
    32161 acgggttttc tgcaaattca ggtagaagga tctgatccag aatttccgat gcctgagatt
    32221 ctttatgccg gaggacatgc tcaacattaa gttgtttaga aatgaactgg ttttttccat
    32281 tacctgcgga atagtgaatg atcaacatgg caataaccat caggatggtc acgctaaaaa
    32341 aaaggagagc attttgaccg tattttgaca ttcttgccat taaatgatct tattagctga
    32401 atttcattta tttaaatgcc gcttgatttc aggccctata acgaattatt ttcaaaaagc
    32461 cgggataatc cttatccggg cactaaaatg gatcacaggg cttaaaatta agaaatatcc
    32521 cgattggata gaatacttca aatattgttc cacgtggaac gttcaacggc caagatgaac
    32581 gatcaggatg gagatatcgg aaggggagac acctgcaatt ctcgaagcct gtccaagtga
    32641 ccgtggacgg atccgcgaca atttttcccg tgcttcaaaa gagagggcag tgaggcgatg
    32701 gtaatcgaag tcttctttaa gtgtaacgga atcgagtcgt tccagtttct gagccagttc
    32761 accctccttg tcaatataac cctggtattt tatcatgatc tctgcttctt cgagaatctc
    32821 aactcgatca ggggcgacac taccgaggta ctcctgcagg aacgggatct ggccggccag
    32881 tccgtcaagg gatatttgcg gccgaagcag aagggtggcc agtttcacct tctgatcgat
    32941 cggggcggtc tgggcattta ccaggtaagg gttgacctga tcgggatcgg tgctggtttt
    33001 ctgcaaaaac ctgaccacct cgtggatctt ttcacttttc gacatcacgg tttgataacg
    33061 ctcggctgaa gccagcccca gggaatgagc cagcggtgtc agccgttgat cggcattgct
    33121 ctggcgcaaa aggatcctgt attcggcccg gctggtgaac attcggtagg gctcgtcgac
    33181 ccctttggtg atcaggtcgt cgatcagcac gccgatgtag gcatccgacc ttttcaggat
    33241 aaattcttcg cttctgtgaa ccttcaggtg tgcattgatc ccggccacga tcccctgggc
    33301 cgcagcttcc tcataccctg tcgtgccgtt gatctgtccg gcaaaataca gcccgggcac
    33361 ctttttagtc tccagggtat aattcaattg caccggggga aaatagtcgt actcaatcgc
    33421 atagccgggg cggaaaatgc gtgcattttc gaagcccgct attttcctga gggcgttgaa
    33481 ctggacatga tcgggcaaag aggatgaaaa gccgttgata taatactctt ctgtttccca
    33541 tccttccggt tccacgaaga gctggtggct ctcttttccg gagaacctgt taatcttatc
    33601 ttcaatcgac ggacagtatc ttgggccaat tccttcgatc cgtccggtaa acatcggtga
    33661 ttcatggaaa cccgttttca gggtttcatg cacctcacgg ctggtataag cgatatagca
    33721 acttctctgc cgggtcaggg gtggagtttt tgtgaaagag aaccgtcgag gatcgctatc
    33781 gcctttctgt tcttccagtt tcgaaaaatc aatagatctt ccatcaactc tcaccggggt
    33841 acctgttttc atcctatccg attcaaaacc cagttccacc aggcattcgg tcaagccctt
    33901 cgctgctttt tcaccaatcc tccctccgcc gaactgtttt aatccgatgt ggatgatccc
    33961 gtttaaaaag gtgccgtttg tcaggatcac tgatctggaa taaatttcat gccccattgc
    34021 tgtagaaacg ccggtggcca cgccattctt taccagaaca ccggtaacca tatcctgcca
    34081 gaaatccaga ttgggggtgt tttccagcat ttttctccac tctatcgaaa acaacatccg
    34141 gtcgctttgg gcccgggggc tccacatggc cggccctttc gacatattca gcatcctgaa
    34201 ctggatcatc gtcttgtcgg tgacaatacc cgaatatccg ccaagggcat cgatttcccg
    34261 gacaatctgt ccttttgcta tgcctcccat cgccggattg cacgacatct gggccatatt
    34321 ggccatattc atggtaatca gcaatacgga tgaacccagg tttgcggcgg cggcggcggc
    34381 ttcgcaacct gcatggcctg cgcccacgac gattatatcg tattctttaa acatccctgt
    34441 aaatgaatgg gaattttaga tcattgatgt tccacgtgga acaaatcatt tatatgagca
    34501 aagataggta aaattcctcc cgggcccgta taaccacccg ctcctcgttc gtcttatcct
    34561 tatagcccat caggtgaagc actccgtgga tcataagacg gcttatctca ttggtaagcg
    34621 aaacatgata ctcttttgcg ttttccctgg ccctgtcaac actgatatag atatctccgg
    34681 caagttcacc ttccgattcc gacatatcaa aagtaatgat atcggtaagg gtaatgtgct
    34741 ggagatattc cttatttaac tggaaaagta tattatcact tgtcaggata taattcagat
    34801 tgttaatctt atgtccttca ttttcaaccg cttgcgtaat ccagtggcga agctttctct
    34861 tgttcttcag ttgataactg atctcctcga gaaaaaagaa tatgtttgat tctttcgcca
    34921 tttattatta ggtcaactgt tcactttctg gtaaactttg aagaaatcat tcatgcattc
    34981 tgcccgccgg ttgaatatgc tttcatcgat cccattgatc ctgaaaagca tttccacagt
    35041 cctgccctca ttccggaagt ttatctcatc ggaaagcttt tgcatcagga acaaaccatt
    35101 tcctgacgtt tcaggagcct tcaggttcgc ggctgattcc agggccgtct taaaatcaaa
    35161 gccctgacct tcgtctgaaa cggtcagcat caatccggct ttaacctttt cctgccggat
    35221 atataccctt tttcgggaat catttccgtt tccgtgaacg acagcatttc tgacggcctc
    35281 tgtcagcgcc atcagaatat ttccgaagta acttccgtaa agcaggtact catcactgat
    35341 ttcgtccaca aattgttcga tccggtacat ctcctccaga gtggagccaa ttgtaaattc
    35401 tttcggtctg ttcatctcct accgcatttc aaaataatag aaatactgat tcactttctc
    35461 tttataataa ggacgcaggt cgggtggtac cgtcctcaac aattcaattt ctttatcctg
    35521 tatcttatta tactctaaaa attgctccgg gtttcctttg ttgaaatctt ttccttcgcg
    35581 tgattctctt ttttcttcct gctccctttt tagctccgct ttttcgtgtt tgagaagcct
    35641 ggtttctatc tcctttaaac gctcaatcgt ttgctgggtt atgattttat taacaagttc
    35701 cgtctcggtt cgttccattt gctccatgat ctcctgaagc tctttactgt tgcccattcc
    35761 ctgctctttc atctggtcct ggtattcccc catcatccgc cggatcgctt cctgctgggc
    35821 cgccatcctg gcaaaacctt cgctctgggc tgaattccgg ctctctttcg tcttcgccgg
    35881 cgtggaacct tcctgcttca tttgctgtat ctgctgattc agcttttgct gcatttcacg
    35941 catgcttttc atcgacatgc tgccttttcc gggcttcggg ctgccgttct tgcactgacc
    36001 tgaacactgc atctgcatag cattcatcat ttgctgaagt gtttccgaca acaacaaagc
    36061 taaatcattt accgatgtca tcacaaactg ctggtcttct gccgcagaag attttctcct
    36121 ctcggtaagg tcttccagcg ccgcttcgac atgatggttt atgtcctgaa tttcccgtgt
    36181 gatgaaaggc tcgatcattt gctgcctttt gctcaaagcc cacagcgaat cctccaccat
    36241 aaccaggtcg tccctgatcc ggttctgggc ttcgatgacc gaaggatact gcgggtcaat
    36301 caccgaaatt tcgagtgttc tgccgatcag ctcctcctgg tcaaacgaaa gctggaccag
    36361 gttctccagg atctcccgca gcgtttctat attttcacca agctcctctt catacatttc
    36421 gctctgcatc tgttccattt tcgatgccat tgatttcatc ccttccgctg cgcttttctg
    36481 cttgggagat gcttttgacg gttttccctg ttgcagctga tcggatccct cttccatatt
    36541 atcctggatc tcctgctcca tctgttcggt gttttcaagc tggtgcggtt cctcaagttc
    36601 tttattcttc tgttcgagtc tgtctagatc atcgcgaagc tgccggaact cttcgtttaa
    36661 tttgtcctgc tcttctttaa gcttttccgc atcggcttta cggtcgtcgc tcttttttgc
    36721 ctgctcatcc tgttttccgg ctaattcctt caatttatct attgattcct gcagcttctt
    36781 ctcaaactcc agttgcttga acaattccag gttccggtcg agttgctttt ccatatcctt
    36841 attgtctttc ttcatctctt caagcatctc attaacctta tccttatcaa cttcctccag
    36901 aagcttctga agttcttcga acatcttctt catctcatcc gtcatgatct gttcgaacaa
    36961 ctcttcaagg cgttgctgct ttcttaccaa ctcctcgttg atctctttgt attcctgctc
    37021 ctggatggat ttcagctcat tttccttctg aatcttttca attgtttcct ggagctcttt
    37081 ctgctggtcg agcaattcct ggatctgctg tttttcctgc catccgatct cctctttatc
    37141 gacaagtttc cggttaagtt catccacttt cttttgaagg tctttcgcat tccggatact
    37201 gttctccagt tcttccataa tctcctggtt gctctcttct gttttttctt ctatctcatc
    37261 cagagtaggg gccctgaaaa tcattggctg agagcgggtt accttgctgc cgttaacagc
    37321 atcattatcc cagacctcga aatggtaaac gatttgctca cccggctgaa tattaatgga
    37381 atcgagttca aagaaatgga agaactgctg ctggttgtgc agtttactga ccggaagctc
    37441 ggcgatgatc tccgggtagt cgttggcatc ggcatctcct tcggggtcaa gccggtaatt
    37501 gaacgtgagt tttgagaaac cgtaatcgtc tttaattaat cccctgaaat atattcgttt
    37561 atcataaatc gaatccctaa cctgttcaac agaaactgaa gggtaggcat cagggataac
    37621 gctaaccgta tatgtcagcg agtcggaatt ccggaggtac tggttcacag aggctactga
    37681 ataggtaacc gatgcatgag cccttttcga aacggaaaat gcattggaag aagggttgct
    37741 cagttccgaa gtttccgtgc cccatctgaa cagtatattc cgcgtatccc gtgtgaaaaa
    37801 ccgccagttg aggacggtcc cctcggggac aatcagatcg cccgtatttt cagatttttc
    37861 atccgatttt ccggtataaa caggataatc cagtgaaatc tcaaagctga gcacaatggg
    37921 tttcgggtaa acagtcagtt ggtattcttc cgatttgaaa aggtcattac tgatgataaa
    37981 caatttggat tcctgcagat tgcggaaatt atagcgaaaa aggaccgggc ttgatctttc
    38041 catcctgtag cggactcctc cggtttcaac aaacaactcg gccggtaatt cttccccctc
    38101 ggcttttaat tccagcagaa aatcttcctg ctgaaatgat tccagttttt cgttgaggat
    38161 ttgaacggta aacggcaggg ggggcaaata aaccgtctga tactttacga tcctttccga
    38221 cggttcggtg atcacacggg gcgacatgat cagcaacagg ataataatga ttaccggagg
    38281 cagggcatac cggagataac ggccgttctt ccggaaatcg atggccaggg caaaaggcac
    38341 cggtctcagt ttttcaattt tctggtcgat gccggcgcgg ataagctcga ttggaaactc
    38401 atcggtatgg tgttcctgca gctttttaag ctgtaaagta ttcaggagct tatcactgat
    38461 ctcggggaaa tgtttgccga taatttccgc cgcctcttcg tgagaaatga tctttccgaa
    38521 tcccagcaac cggataacgg gcaccaggat ataataccac agaaaaccgg cagaaaggac
    38581 cagaaatgaa taaaaaaaga gagtccggat gccggggctg aaccgtccaa aaaactcggt
    38641 gagcgttatg aggagatagg ctatcgttac aatggccaca taaaatatgg ctcctctgat
    38701 cagccggttt cggtaaaact tccggataaa ttcatcgagt ttacggatca atatggaata
    38761 gttgtcttct gtcatgagat gcaagccttt cagggtatag aaccctaacg gtaacgaaca
    38821 aataaatatt atctttgttc gcttatacaa agttagttat agtttcacga ccttccggtt
    38881 aaattatttt ttctatgtta aaaagactgg tttttctttc tattctgatc ccgttaacag
    38941 gaattctgat ggctcagtac aatcatcccg aaccggatga tgcaccttac tgttatcatg
    39001 tcaaatcagc ggcaatggct gcgaatgaat attatgcgcc cgttgtgaca aatccattgc
    39061 tggaaaaata cgatgttacg ttttatttcc tggacctgaa tgtggaaaac gatacggtag
    39121 ccgtcagcgg aagtgttacc acaaaggcca tagtgaccgg tacccagatg gatacgatag
    39181 tttatgaact tattgacgag atgacggtag atacggttta tgtcaacggt atccagcatt
    39241 cgttcgtcca caataatgac ctgatcctgg ttccgatcga accaatccca aacggcgttt
    39301 attttacgac aaaaatttgt tatcatggta cacctccaac cggcggcttt ttctcagggg
    39361 tgacgacggc ttacagcacg ccatttgaaa aaaacgttac ctggacctta tctgaacctt
    39421 atgccgcgaa agactggtgg ccgacgaagc aggatctcac cgacgatgcc gactcggttt
    39481 gggtatttct gacgacgagc gccgaaaaca aggcgggttc gcagggactg ctgactgccg
    39541 ttacaccggt acccggcggc aaactccgtt acgaatggaa aagttattat cccattgatt
    39601 attacctgat ttcctttgcc gtagccgatt accaggaata caacatttat gcttatccgg
    39661 aagggacgga agattcggtc ctcatccaga attatattta tgatcatgta aactgcctgc
    39721 cattatacca ggtgggaatt gaccggacgg caggttttat tgagcttttc tccgatttat
    39781 tcggacttta tcctttcatc gatgaaaagt acggtcattg tcttacccaa ctaggtggcg
    39841 ggatggagca ccagaccatg acaaccatcg gcgctttcaa tttcaacctg gtagcacatg
    39901 aactgggcca tatgtggttt ggcgacaaag taacctgcgc gacctggagc gatatctgga
    39961 tcaacgaagg attcgccaca tacagcgatt atcttgccgt ctccaactta ctgtcgcccg
    40021 ccgaagattc ggcctggctg agaagcaagc acaaccaggt gaagagcgct cccggcggca
    40081 gcgtatatgt gccggaagag gaactcgaca atatctaccg gattttcaac ggcaggttat
    40141 cttacagcaa aggggcattg ctgcttcata tgatccggtt cgaattacag gatgacgaaa
    40201 tgtttttcaa tgtactgcgc acctatatgg aagaatacgg cgattcggtg gcaacgggcg
    40261 ctgatttcag ggagtggctg gagtatatat cgggtgaaga ttttaccgac tttttcgacc
    40321 agtggtatta cggtgaagga tacccgattt tcgatattgt ctggaaccag aataacggga
    40381 acctgaacgt ttcatcgacc caaaccacat cgacaactgt aacgcagttt ttcaaaatga
    40441 aagtccctta tcatgtggtt tattccgatg gtaccgacac cacgctgatc ctttaccagg
    40501 gtgaaaacct ggaaacctat tcgatcccgg tttcaaaaga ggtcgaacat attgaactgg
    40561 atcccgaaca gtggatactt caccacctga acagcctggc cgtggatatt gaagagcgga
    40621 acaacccgct ctattttacc gtcgggccca atccggcgca tgattatttc agggtatttt
    40681 tgctgtcgaa tccggaaaaa gaaatgcaac tgtcggtgac cgatctggcg gggaggactt
    40741 tactgacaca aagcataacc ggacgttcaa gtgccattga tgccgggcac ctgaaacccg
    40801 gtgtttacca ggtgattgtg agtgacggca agggaagaat gaaccgaaaa attttaattg
    40861 attgatgaaa gaagtcaggg taagatttgc gcccagccct acagggccgc tgcatatcgg
    40921 cggagtaagg acggcgctgt acaattattt gtttgcaagg cattccgggg ggaaaatgat
    40981 cctccggatc gaagatacgg atcagaaccg ttacgtaccc ggagcggaac aatatatcat
    41041 cgattcgctg gagtggaccg gtatccggtt tgatgaaggg gtgcatgtcg gtggccctta
    41101 cgcaccttac cggcaatcag accgcaaaga gatctaccgg cagtatgccg atgagctggt
    41161 gcgcgccggg catgcctatt acagttttga tacggcggat gagctggatg caaaacgaaa
    41221 agcggcggaa gcctcaaagc aggttttcac ctacgattca tcggtaagga acacgatgaa
    41281 gaactccctg acaatgaccg gtgaggaggt ccaaaagcgc ctggaatcgg gagaaccgta
    41341 tgtgatccgt ttcatgatcc cggaaaatac gacgctggaa ttcaacgatc ttatccgtgg
    41401 cgacatgacc gtaaacacag cgactttaga cgacaaggtg ctgttcaaat cggacgggat
    41461 gccgacttat cacctggcca acatcgtgga tgaccacctg atggagatca cgcacgtgat
    41521 ccggggcgag gaatggctcc cttcgctgcc cctgcatatt cttctttacc gggctttcgg
    41581 gtggcaggcg ccgctgtttg cccatcttcc gctcatcctg aagccggaag gatcgggcaa
    41641 gctgagcaag cgtgacggcg accggctggg attccccgtt ttcccgctgg aatggaccga
    41701 tccctttacc ggcgacgtct cctcgggcta ccgtgagagc ggctatttcc ccgaaacggt
    41761 ggtcaatatg ctggcattgc tgggctggaa cccaggcacc gagcaggaga tcttcagcat
    41821 ggaggagctg atcgggcagt tttcgatcga aagggtcggc aaatcgggct cccggttcga
    41881 cccggggaaa atcaaatggt tcaaccatca ctacctcatc acaaaaacca atgaagagct
    41941 gacggatctg ttctgcaaag tgctggaaga taaaggcatt catgctgaac gttcctttgt
    42001 ttcccgtgtg gttgagctgg taaaagaccg ttccgatttc gtcggccagt tctgggatca
    42061 gtcgtatttc ttcttccgcc ggccagagtc gtatgatgcg gatcttgcca ggaaaaagtg
    42121 gaaagaagat acgcccggtt tattgcgggg ttttgccgaa gcgttgccgg ggctgccaga
    42181 ttacagggcc gaacagataa aactctttgc cgagtcttat gctgccggaa aaggcgccgg
    42241 cctgggccag ctgatgaatc cgctgcggct ttgcctggtg ggagggagtt ttggtcccga
    42301 cctggccctg atctgcgaga tgctgggaaa gcaagaagta atggatagag ttcaccttgc
    42361 actgaacttc ctgaaaagtt cgggaacaat tgtatgatat tatggggact attgcaatag
    42421 aagggatgga gttttatgct tatcatggtt gttttcatga ggagcaaatc atcgggacac
    42481 gttttacggt ggatcttttc atggaagcgg atacaacgag cgctgaactg acggatgatc
    42541 tttccgaaac agtggattat caatctgttt acagggtggt ttcggaagag atggccatca
    42601 aatcaaaact gttggaacac gtagcccggc ggatcctttc ccggatcgga cgcgagtttc
    42661 cacaggtcgg cgctgcgcgg atcaaggttt caaagatgaa tccgccggta ggagggaaga
    42721 cggagagggt gagcgttgag atttgacgat ttggagattt agagatttga cgatttttcc
    42781 aacttttgac gactgatgac caaaaccaac gaccaagaac cttcaacctg cccaaaatgc
    42841 ggcaagcagt tttcctgcgg gaaatcagga aagtgctggt gctacgaggt gtatgtgccg
    42901 ccggatgttt tggaggaaat tgaagcccaa tacgaccgtt gcctttgccc ggattgtctg
    42961 aaaaattata atcagaataa aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43081 nnnagaatta tttctttccg agtgttgttt cgaatttttc gcgcggcact ttgatctttt
    43141 cggggccgaa tgtcagctca tcgatcaggt tctgtgctcc ggcgtattta tcaataacga
    43201 aaagcacata acggatatcg acgcagatgg tacgttgcag tttgggctca taagcgatat
    43261 tgccgctcat ggcttcccag ttgccatcga aagcaagtcc gataagttct ccgttgccgt
    43321 tgataaccgg gctgcccgaa ttaccgccgg tgatgtcgtt tgtggtgagg aaacaggtaa
    43381 ccaggtcatc cttacatccg taagggccga agtctttctc ttcaaaaagc tttttcagtt
    43441 tttcgggaac gataaattcc tcgttcgccg gatcttcttt ttccatcacg ccgctgagat
    43501 gggtcttata atcataataa accgcatcgg cgcctttata atcctcaacc gtgccgtacg
    43561 taagcctcat ggtcgaattt gcgtccggat agaatgcctt ttcgggcatc atttcgcgca
    43621 agccggcaat aaacagccgt tcatatttac ccatctcctg gttggccgaa cggtaatcgc
    43681 ccgacaggtt catcatggag cccatcagat cgttgctgag tttgatggcc ggatctttct
    43741 gaaaggtttt caggctgggt ttgtcgagga aggcatttac ctttgcctct gaagagagga
    43801 atgatttggc gaaaatatct gcggccatgg ctttaaaatc tcctttatat ttggccacga
    43861 ggtctttaaa tccctgcggc tgcatttcta ccggcatctc cttcgagtaa atctccatca
    43921 tccgtgccaa aactttctgg tcggtcggcg cattgtaatc cttgaaaaat tcttttgccc
    43981 cttcgcgaag cgattccgat tcggccttta aaccggcctc atcttttcct tccaggtaac
    44041 cgccaacacc tgaaaaattc tgcgcatagc ccaggatctc gggggatgag gctcctaaag
    44101 cagccaggat gaagggcccg aatgatttgc tgatcttttc ataaccggct tttatccccg
    44161 gcagaacgtc gccgtatttt tccttcctgg cggcatcttt ttccacccat gcggtgaact
    44221 gtgattccag ttcttccttc tgggatttaa catccagcct cttgagccct ttcagttcgc
    44281 cgatatagaa tttccagcca ttggccgttc cggcgtattt ggaggcatat ttgataccga
    44341 cttcgcggtc ggcagtcatg tcttctttcc agatttcgag ttgttcgccc agtagcctga
    44401 caatattggg atcgaaatgg ttgatctgaa aatcgacgcc ataggaggtc aggtagcggt
    44461 cggtgctgcc cggatagccc cagatcatgg caaagtcgcc tttttccacg ccattgaggg
    44521 taaccggcag atggtgcttc ggtaccatgg gtacattttc ttttgaataa tcggccgggc
    44581 tcccgtcagg ggcagtgtaa acccggaaaa tgctgaaatc gccggtatgg cggggccaca
    44641 tccagttatc ggtatctccc ccgaatttgc caatggccga agggggagcc ccgacgagcc
    44701 tgacatcggt ataaacctca taaacaaaca ggtagtactc attcccgttg aaaaaactgg
    44761 tgacattgac aaagtatttt ttatcgggat tagcggcttt tttcagcctg gcggttatct
    44821 cgcgcacttt ttcagaacgt tttgaggccg acatggtatc gctgaggaac ggaacgattg
    44881 aatcggtcac atcttccatc ctgacaaaga aagtagcgct caggtcttcg ttcgggagtt
    44941 cttcttcctt gctcatagcc cagaaaccgt cgttcagata atcgtgttcg atagagctgt
    45001 gtttctggat gatatcgtac ccgcagtgat ggttggtaaa taagaggccc tggcttgaca
    45061 ccacttccgc tgtgcagaag aatccgccgg gctggctgcc gtttccaagc ccgacgatgg
    45121 cgtctttcag gctggagtta ttgatactgt aaatctcttc tgcagtgaga tggagtccca
    45181 ttttctgcat atcgacatag ttcagtctct ccacaaaaat ggggagccac atcccttcat
    45241 ccgcccggag tgagggggca gacagaagga taaaaagcga aaaaaagata gtaaagcgtt
    45301 tcattatcat tttattaagt tattttaata atattttacc acgtagtaca cggagttaca
    45361 cagaaggact cggagtcgat aacggttgaa ataaattgac cgaatgacta aagccccagc
    45421 atttgttttc ctttttcaaa cactacatcc acggggattc cggaggcatt gatccggtcg
    45481 aggtctgttt gcagcgccgg ggtaacgatc ccttcggtgg ccacccagtt tttcgcggct
    45541 tcataatcgc catcaccctg gatgtagata attttctgca ccagtttttc catggccgct
    45601 tttgttttgt cgaaatcgac cgtataaatg ccctcttcgc ttctggtaaa agcgccttcc
    45661 gaccggaaga agttaaagcg catcatattg gccttaccgt gtgcgcttgc cgctccaaac
    45721 ctggaggagc ggaagatacc ggcaaagaaa gtcacataat tgtccatcac ttcgccttcg
    45781 gtcagttcac ccatttcata gagtttggta acgaggtaga ggcccatgat atcggctttg
    45841 ccttcctcga tggatgaata ggtctctttc agagcggtac gggccggtcc tttgccgttg
    45901 atggtatttt taaccccaat ggcgtgtgcc acttcgtgga acatggtgtt ctcgaaaaag
    45961 gcgttgaatg tcacgtgctt acgctgggcc ggatcgatca ggagttcggc tatcggcacc
    46021 atgatcttat cgaatttggc ttgcatcgca tttttcagtt gcaatttccg ggaacctttc
    46081 tgaagctgga cctcttcgtc gttgggcagg ttgatggcga tggttttgct tccggcgttg
    46141 caatcgccgg catagtaaac aacgtcatat gcattcaggt cggcgtcggc accggggatt
    46201 tcggttttgt actcctctct taccgggaga ccacgctgca ggtcgggtag catggcggca
    46261 tatttgacga gcctggcgct ccagaccggg tctttaacca ggataaagga ttcgaaggcc
    46321 gctttgtagc catacaatcc gtcttcgtaa ttttcgattg gtccgacgac gaagtcgagt
    46381 gccgagttac gcatctccat ccaggcaaag tcgctgggct ggtaatcgct ggtcagaagg
    46441 gcatcggcac gcagttccag gtattttttc aatccggcat cttcggccag tgcggccgct
    46501 tgtttaatga gttctgcggc ttttttgagc tgggtttcat aagcctggtg gtaccacacg
    46561 ctgcggagtg agccgtcggc attgcggcgg atcagggtgt agaggctttt cttattggga
    46621 tcgctgaagg cttcaaattc ttcctttgtc atatcggccg ggtaaaaatt agcgccggca
    46681 ggtttttcgc cgattccgtc gatgaaggaa ttattcccgt cgagccggtc ccaggggccg
    46741 taattgatac gggcaaactg ctttgtggtt tcgtcctgga tacgtgccag gaaaggctct
    46801 ttcgggccca tggcctgaag ccagaacagc tcgtccatga tgtccgccac atcgaaaaag
    46861 atagccacga tctgcttttc gttagccgtg agatggctga tgtcggtggt aagggtaaac
    46921 ggcgcaaatg tagcgagccg gtttgcaaca tgctcaggag aggccggagc aagcggttgc
    46981 tgcccggtaa cggtcccggg atactgttgt tccatctgga attcggtttt ctgcggctgc
    47041 tggcaggaga cggcaattat aaccgtaaca accagcagga tttttgaaaa tacgttatta
    47101 agcatgatat tggtttttta ggaaatttga acggcgaagg tacttaaaat ctgctttcac
    47161 agccctttcc gtgatgaaaa tcgactccaa acgatgatta tttaagggat gttccgggtt
    47221 ttttgatttt atcctccgga gtcagacccg atttaatttc aatattgatc ccgtcggata
    47281 atcccgtttt gatatatctc ttctcgaaca tttggggagc cgtttccact tctacgtaag
    47341 ccgagtctcc ttcaaagaga agcaggcttt cagggatggc cagcacgctg tcggtccggt
    47401 cgagcaccac ttttgcattg gcgctgtagc ctgccctgat gaactggtcc tgtttcagcg
    47461 aaacattggc tttgatctcg aactggatgg ccccgttttc ttcaatgcct ttcggtgaaa
    47521 tgtgttccag caccgcatcg aagctggcat cgtcgatagc gccgatggtc agtatcagcg
    47581 gcattccttc cctgatcttg cccacttcgg tttcatcgat cttaccttcg aagatcatat
    47641 cgcccatatc ggcaaccgaa gcgatggtgg taccgtcgtt gaacgtattg ctctcgatca
    47701 ccgaaaaacc cacttccacc ggtacatcga ggaccattcc ctcggctgtg gagcggacga
    47761 tggtattggt ggcctggccg cctttcttgg tctggccttc tttgatcagc tccaggttgt
    47821 tctctgccgc ctcgacctcc tccagggcat ttttataggc aatttcatag ggttcgtatt
    47881 ccgctttcgc gatgacgccc tgtttcaaca aatcgccctg ccgctcaaac tgcctttttg
    47941 catcttccag cccgatcttt gccctgttga cgcgcgattg cgcattattc agcgtaacca
    48001 tatcgggaat gatcttgatc ctggcgatca ggtctccttt ccggatcata tcgccggctt
    48061 cgacatagac ttcttccaca attccggaga cctgcggttt gatcatgatc tctttcctgg
    48121 gcaccaccga tccggtagcc acggtacttt ttatgatttc cgttttgaag gcccgggcag
    48181 tttcaaacac caccggcttg gcctgcgatt tctggtaaag gaagtagatc gtgtagacaa
    48241 agatggatag gacgatcact cctgccagta ttttcagcgc ttttttcata gttggttgtt
    48301 ttgatgagac gcaacgtgtt gcgtctttac tctgttgttg ctatctgttg atgagacgca
    48361 acatattgcg tctttactct gttgctgcca tctgttgaga cgcaacatgt tgcgtcttta
    48421 ctctgttgct gccatctgtt gagacgcaac atgttgcgtc tttactcnnn nnnnnnnnnn
    48481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48541 nnnnnnnnnn nnnnnnnnnn nnnnnnncgt ctttactctg ttgctgccat ctgttgagac
    48601 gcaacatgtt gcgtctttac tcatctctga gggcgtcgat gggtttgatg ctgatggcgc
    48661 ggcgggcagg tatcaggcct gccagtatcc ctgagatgac caggattccg agcgagagca
    48721 tcgccatatt aaagttgatc tcggggttcc ggaacatctc cgattctgca ccgcttttgg
    48781 tgatcatgaa gtcgaccagt tcgatcagcc cgacgcccac aaccaggccg atatagcctg
    48841 ccagagtggt taaaaagacc gattcgatga cgatctggct cattatcgct gacggcggtg
    48901 cgccgattgc ccgttgtatc ccgatttcct tggttcgttc tttaacgacg atgagcatga
    48961 tattgctgac gccgacaacc cccgcaaaca aggttcccga tcccacaatc cacaccagga
    49021 tggtgatccc ggcaaagagg ttactgaatt tattgaattc attttccata ttccaggaac
    49081 cgatggcgcg gttgtcgtcg ggatggatat ggtgccttgt tttaagataa tcctttgtct
    49141 tctgttcagc cacggaagcc ggaacccctt ttttcgccgt aatggagaac cagccgataa
    49201 tatcgccgta gttgtaggtc tgctgcaggg tattcagcgg catatggatc gccttttcgt
    49261 cctgctcggc ttgcgggcct ctctgtttgg attttaccac gccgatcacc ttgaaataga
    49321 ctcccgaaat ctgcaggtat tccccgatcg ggttttcatc ctttttaaac atctcctggt
    49381 aaaccctgtc gccaatcacg atcaccttgc gttgttcctc caggtcattc tggttgatca
    49441 cccggcccat ggtcacgttc atcggcatga tctcgaagaa ttcgggtgta tcgccgaaag
    49501 tggagaatgc gccggtcctt tctccgcgca caaccgtgct tcccgctccc ctgaaaccgc
    49561 ctccctgcaa tcgtggcgcc actttatcga cttccgggat attcagcctt acagcctcgg
    49621 cgtcggcatt cttgaaatta taccaccggc cccgctggaa tcctttatag ggcattgtgg
    49681 tacgctgggt ccacatgaaa aagctgttcg tcgccatatt gccgaagcct tccagggccg
    49741 aattcttcag tccgtttccc gaacccatca tcacgacaag gagaaaaatc ccccagaaaa
    49801 ccccaaaggc ggtaaaaaag gtcctcagtt tgttcttttt cagaacaatc acgatttcct
    49861 gccatttgtc gtaatcgacg atactcatat attgatattc ataattttca aataaggcag
    49921 ggggataaaa tagcatccct cagagacccc ctgcctatgc aagcattaac catttgctgc
    49981 attatctttg ccaggagccg ttgcatgcaa cggctccgct gttattcata cctgagcgct
    50041 tcgatcggct ggatggccgc ggctttacgg gccggaacaa aaccggcgag catgcccgat
    50101 accaccatta cgatcgtcgc tcccaaggcg ataaaaatat ccacttccgg attcttaaag
    50161 aacgtatcgg aattattaaa tgcgggtgaa atcagctcaa gcaatcccac acccatgacc
    50221 agaccgacat agccggcaaa ggcggtaatc agtatcgatt ccatcaaaac caggctgacg
    50281 atggaccatg gcgttgcccc cattgctttc cggatgccta tctcctgcgt ccgttccttc
    50341 acaacgatca tcatgatatt gctgacaccc accacgcctg caatgatcgt cccgatgccg
    50401 atgatccaga taaacatccg gatggaggca aacagggtca tcaatttttt gtattcttcc
    50461 aggttattcc atacaaacac cgcccgttga tcctccggat cgaacttgtg tttttgcgcg
    50521 agggtttgcc tgacatcttc tgccatttca cggctctctt caaccgtgac atccttcgtc
    50581 accacgctta cgttgaaaat cctgttcccc aggttaaaaa cgcgctgcgc tgtggacaag
    50641 gggatataga cacgctgcat atcgcggtcg ccgccgacat cgctgaatac acccgcaacc
    50701 ttgaagggga caccgctgac ctggatgtat ttaccgattg gatcctcatc cttaaaaaga
    50761 gcttccttta caggtttccc gataaccacc gacttgcgga aatccctgat atcttcttca
    50821 ttaataaatc ttccaccggt aatatcaatg gtttcataat aaccataatc gggatggcag
    50881 gcaatgatat cgaaattccc gtattcttta ttgtaagaaa tggtgttgtt ctgccaaacg
    50941 ttcacccggc ctgaaatctt gtaaatattg gggatttcgc ggctgatcag gtcataatcc
    51001 tcattggtga actgaatgac cctcccggct ttcagtcctt tgtaagccgt gctggtctgc
    51061 ccggggttga tccagagggt attggtcgca tcgccttcaa acgctttttt gacgccattt
    51121 tcgagtccat tgcctgagcc cagcaggatg atgagcataa agatgcccca ggccacgctg
    51181 aatcccgtca ggaaggtgcg caacctgttt ttcttcatgg tctcgaagat ctcctgccat
    51241 ttatcccagt caaagattcc cattgttgcc ggttatgttt agacgttgag agacgttgca
    51301 tgcaacctag acgttgcaag caacgtcttt acttaatatt agttgactct atcaagatac
    51361 tgatttttat tttttccatt gacttctgtt cgctcgatca ggccatcctt caggtggatg
    51421 atccggtcgg tcgaatgggc gatatcgtat tcgtgcgtca caatcaggat cgtgatgccg
    51481 ctctggttga tctccttgag cagatccatc atctccatgg atgttttgga gtcgagcgcc
    51541 cccgtaggct catcggcgag gatgatccgg gggtttgaaa ccagcgccct ggctatggca
    51601 atgcgctgtt tctgaccgcc cgacagttca ttcggcagat ggtgccacca ttcttccagt
    51661 cccatctttt ccagcatttc catagcggtt ttgttcctct ttttccggct gatcccctta
    51721 tagtaaagcg gtaatgccac gttttccatg gcgtttttga aagagatcag gttaaacgac
    51781 tggaaaacga aaccgatcat ctggttgcgt aactgggctg cttttttctc cgtcaggtcc
    51841 cggatcgcaa ggccatccag gatatattcc ccctgatcgt ggttgtcgag gatgccgata
    51901 acatttaaaa gggtagattt tccggatcct gacgatccca tgatcgaaac cagttctccc
    51961 tttttaatgt caaggtcaat tcctttcagc acgtgcaggg aggttttgcc cagcccgtat
    52021 gatttgttga tgttgcgcaa gctgattatt gtatcttcca tcatggttaa tttacaaggg
    52081 aaatgggttt tcccatatag aaatccaaaa tagtagtcgt atagatgaag cggtattttg
    52141 cctgcaggag ttcggactcg gaagccgcca ggttcttctt tgtttcatta taatcgacgg
    52201 ctgttacaag cccgacagcc agtttctgtt cggcatattt gaatgcctct ttctgtgcat
    52261 tgactttttt ctctgaagca ttgaaatttt tcaaagcggc aacggcatcg gcataggcct
    52321 gttgaatctt cttgtcgaga tcgagtttag cctgggccag gttgagatcg gcctgttctt
    52381 tctggatctt tgcctggctg atagcattgc gcacctgcca gccgttgaaa atgggtatct
    52441 gcaatgagaa ttgtaggctg gtattctggt tatcttcaaa ctggtctctg aaagagggaa
    52501 tatagaaatt gcctgcggat gtagtgtagt cggaaaccac aatttctgtt ggattgcttt
    52561 gcaggtaacc gatcggaacg ccggttatat taatggtctc atcggtaggc acctccaggc
    52621 cggaataacc ggtagccagg gttccggaga aagagatcgt gggactgagg tatccgcggg
    52681 caatggagat tcctttttca gcactcctga cgcgcagttc ggcgcttttt atctcggggc
    52741 gctgggagag ggctacatcg taaacgtttt gcggtgtcag cacaatttga ggcgcctgga
    52801 tcggtttcag cttcggggtt tcgattcgga aatcgggtga atacggatga tcgataagct
    52861 gctgcaggct caggtaggcg atggtcagcc tgttttcggc ctcgaccacc tggagttctt
    52921 cggtagccag ctgggcttcc aggttgagca gatcaccccg ggctatcgtc cccgcttcga
    52981 ccattttctg catccggccg acctgctgcc gggtgatccc ggtttgttcc tgcgaaacgg
    53041 ccagcagttc ctggctgtag agaatgtcaa gatagtatcc ggctacagcc actgagatat
    53101 cgtccatcat caggtcaaga tcatactgaa ttgcggcata atcgagtttg gcctggttca
    53161 cggtgttgat ctggcgcaat ccctggaaaa ggttcagttg ggttcccagg taaaaattat
    53221 tcgactgaac gcgttttgtg gcgaattcgt tggtgtacct gtcaatcgtc tttccccagt
    53281 tgtaaccgtg ggtggcgccg gcattgaggt tgggaagcat gaccaggtag tcctggagca
    53341 gttgggcctt ctggctttcg accatcaaca cctgcttttt aatgttgatg ttgctgtcga
    53401 gggcgaaatt gatgcaatct tccagtgtaa actctttcac ctgggaggat gcggccaggg
    53461 aaaaaagaac caggaatgcg gcggtcaatt ttttcacaat gtttttaaga acgctttttt
    53521 ggttaatgct tttggtgtac atagttaatc tgagttagat tattttttta agtaacgcga
    53581 ctttgcagcg aaaaatatgc cagtatcata atttattgat atttaatatt ataaaatgtt
    53641 gtatgatgat atcgttaaaa aagtcgtgtt cgccagctaa caacacttgt tcgataacga
    53701 acaattaatt tgcaaatatg gcaggagaaa tcaaaagaat tcgcgcctga tttgcaacag
    53761 ggcgggaaga ctttacgaat ggcagcaatt gggaattcag tgtcttttac cggttcgaga
    53821 gtgctttgat ggcgtcaccg gcggccgtca ggaccggccg gatcgaaaca ggctgtccga
    53881 ccgcctgttg catccacacc tgcggcgcca gtaagcccag tgtgtacatc cggtagactt
    53941 catcgggcag ttttttgcct tccatgtact gttccacctg gaactggatc aggtggccca
    54001 cgggataagc cgacaggtag agggggttat cgatcatatg cgaataaata gccaggatgg
    54061 gtgtgtcctt aaccccgaaa accggtgcat aatagctatt ccagacatcc tttgcaatgg
    54121 agataacggc atctttcagt tgcgggacgg tcgcatccgg gtgttcgtac aaccatttcc
    54181 agacgcgcat atcgaccagg gatacgccca tgatctcata attggcccac aggttgtcga
    54241 gtgccatcag gtggttctcc atcggatctt cccgggcaat ccccagcacg gccaggtcgc
    54301 gccgctggaa caggaaagcc agtgcctcgg taaaagccgt gttgggcacg ccgtgaagaa
    54361 tgtaatgatc gatattgtag agcgaaaggg tttgctcgac attatgcccg aactcgtgca
    54421 tggcgatatt gtagcctttg tagtccattc cctgctcccc gatccgggta cggagccggg
    54481 cgcggtcggt ccgcatttcg gcgccccagg catggccgga gccacgtgcc ggatccaccg
    54541 ttatccgcga agcgatgatc gcggccgaat ctttgtcaaa tcccagtttt accaggatat
    54601 tcggcagatc tttctcaaag gccttattat ccggatactt agcgcgggtg atcttattca
    54661 gctccgcttc gttgagagag gtgcctgctt tgaaaccgtt gtaccagata tcgaacggtt
    54721 ccagtggccg cccgagcctc ttgctgataa gctctccggc ctttttaact tccggagagg
    54781 agaccagttc gacgaaaagt ttttcgacct cttcctgtgt gatctccatc ccttcgtcga
    54841 aggcgcggct gatctggttg ggataaaaag gctggtaggc atcggtggcc gataaggctt
    54901 tgaagttttt cagcaacact tcgtagcgcg ttcccggctc ggctgtgttg tcagcagcgg
    54961 catctccctg aaagacctca ttggagatcg gattccaggt cacttcgttg ttgttgatga
    55021 caactgccgg gatctcctgg ttgatgatgc gaagcataac ctggtagatc aacttttgct
    55081 tttccagtcc accttcctct ccaaagtgtg atttcagttc gtcgcggatg ccccagtggg
    55141 agatcagccg gagattatcg gggaaaagct gccgcccttc attatccctg agcttgtcca
    55201 tacagatgtt gtacccggag atgtaattat cggcgtcggt cagggttttg ctgatattct
    55261 ggttcaaaga agccggcacc cgggaggtga acagttcacc catcctagca taggcccatt
    55321 ctttccgcgt ccagccctcc cctttatcgg tcttttcttc caggttatgg aacggaaaat
    55381 tgagcaggac gataaaggcc agtttgttct cgaacatatc gtcggtaaaa tgggctcccg
    55441 gattgtaacc tcccaggatt tcatccaccg cgtggatatc gccgagtggc aggtgcagcg
    55501 ggagcttcag gtcgacgctg atcttgttgt aacaaccgcc gagggattcc agtgcagccg
    55561 acaaacggtt aaaaacaagg tcgagttccg cttcattacc gataaatttc tccatgcaga
    55621 acgctttaaa ctcatcggtt gtgccgtcgg tgccattcca gaagtcggcc gcctgtttca
    55681 caccggtttc gatgcgtaaa gcctggtttt ggccatattt ttcttttaat gcgcggacga
    55741 catcgctccg ggacgcttca tcgacaatac tcagtttcat ttcggtgctt tcgttagcgg
    55801 gttgtgttgt tggcgtggtg catcccgcca gcagaaggat aataaataag aggattccgg
    55861 tctggttttt catcgtgtct gaattatatg tttcagccac gaaattaagg aattatttcc
    55921 ctttacggaa atcctggaat aagccaacga cggggacgag gcttctccgc cgggaatacc
    55981 ggaaataccc ttccctggcg gtttttacat cctcgatcgt cacaaatgag tgtggattgt
    56041 gttcctggat gatcttcatc acctctttca ggtcaattcg ctttacgatc gtaaatatca
    56101 ttttttgttt accggtcatc ccttcaatat cgacgagtga agcgccgaat tcctcttttc
    56161 gcaggtgatc gaccaggccg gtgatttcgg atttggagac gatgcggata aggacggtcc
    56221 ccagcgaaag tttttcatcc agatatatac cgatgtaatt gccggtagca aaaccgagcc
    56281 cgtaggcgat atagcacaaa atattatcca ggtgctgcat gatctgcccg atggccagca
    56341 accagatgat cacttcgaaa aatcccagga tcggggcata gaacttgaat cctttagaga
    56401 caaatatcag gcggagcgta ccgatgctca catccaggat gcgggccaga aagatcagca
    56461 aggggaggat aacccaggtg aatagcggtg aatcggtcag aaatattgtt tccata
//
LOCUS       DAOPNH010000003        52897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_520_length_52897_cov_7.724083, whole genome shotgun sequence.
ACCESSION   DAOPNH010000003 DAOPNH010000000
VERSION     DAOPNH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_520_length_52897_cov_7.724083"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1178)
                     /locus_tag="PKZ74_00440"
     CDS             complement(108..1178)
                     /locus_tag="PKZ74_00440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM91243.1"
                     /translation="MYFRILDLSNSEIWGNYLIRLPNKQQDIYYTPGYYQLYEDLGDG
                     KALCFLFEKDENLALYPFLINPINNLNFELNNTYFDIQGAYGYNGVLSSSNDPAFISD
                     FYNSFNSFCIQENIIAEFTRFHPILQNELFSLNDMSTIRDRETVMIDLKKPYADIWDD
                     DYSSKNRNMIRKAIRLGYICQIETSPGKDHIDQFISTYHYSMRQAGAEPYYYFNKDFF
                     NNMFKYLKDKIYLFNILNQTGEIICSSIFLHYNDYFHYHLSGRNENADNAVNNFLLDS
                     AVKFAQEMGAKLFHLGGGRSNSEDDSLLKFKRNFSGITIPFHIGKRVVNEKVYNEVVR
                     QWENKFPEKVEQYRNMLLKYRF"
     gene            complement(1407..1775)
                     /locus_tag="PKZ74_00445"
     CDS             complement(1407..1775)
                     /locus_tag="PKZ74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM91244.1"
                     /translation="MNPKGKIITLRAIEKNDLIDLQRWSNDQEIQYWLGGWHFPTSMD
                     TMEKWLDRINSDNLNQRFAIDHVDLGLVGTTNLVEINWKDRNAFHGMLIGDKDIRGKG
                     IGVDVVFTIMRYAFEELGLQ"
     gene            complement(1855..2925)
                     /locus_tag="PKZ74_00450"
     CDS             complement(1855..2925)
                     /locus_tag="PKZ74_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91245.1"
                     /translation="MYIGGEHTFSPGLLFKPSNNSFDNLISDLEKHYFINYTFGGYYS
                     LLAILDHLKSDIDEESEVLLPSYLCQSILQPFKARNIKYNFYRVDQNLYVDISHLLSL
                     INKNVKAVLFIDYFGATQKDRLSTTIDFLSSKNIFIIQDMVQCLHLDQDKMFGDYIFN
                     SFRKFFPIEGSVLLSKKEININFSKRTCSKFVLNKRMGQLLRYFHVNYGMFSSSSFLK
                     FFKTADKNYYDEQNYKMSKTSRAILKKFDIDLLIKTQKQYYQFLLDNFGNYVPELLNN
                     QRFVPLGFVIVVPERDLLRKKLFLENVYPPVHWILSDEIDDQLFSESIALSKSILTFP
                     LLNMNDNKFDFMIKKMLESFKD"
     gene            complement(3074..3961)
                     /locus_tag="PKZ74_00455"
     CDS             complement(3074..3961)
                     /locus_tag="PKZ74_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91246.1"
                     /translation="MLLLGITFLFDYAIGTTLKHYYFTQTSGAQYRTTYSMDSTNAQV
                     LIFGSSRANHHYNPSIIENMTGMSCYNTGRDGNFLLYNYAIFKTVINRYKPEIVIIDV
                     EKDELSSTIDDYERLSSLLPYLNRNKAIDQTIALRGPYEKIKCLSKIYPYNSLLLTIA
                     IGNTELNKARKPDIKGYVPLNNIWDNPYPELDSMVVKTIDQNKKNALLEIIQDCKLHG
                     IRLVLVISPLFIKSVDTTGGNPVMQIALKNNVEYLDFTSDTIFLNNHHYFTDPEHLNE
                     SGATLFSTLLIEHLLRGEK"
     gene            complement(4012..5400)
                     /locus_tag="PKZ74_00460"
     CDS             complement(4012..5400)
                     /locus_tag="PKZ74_00460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM91247.1"
                     /translation="MTTLYFVLPHRFRWFLLLAASCYFYMAFIPIYILILGFTIVIDY
                     FAGIWLEKTEGKKKKYFLIASLIANIGVLAVFKYFNFLNENLSALLNSFGYQNSIPNL
                     SILLPIGLSFHTFQAMSYTIEVYRGHQKAERNFGIYALYVMFYPQLVAGPIERPQNLI
                     HQFYEKHYFDYQRVVEGLKLMLWGLFMKLVIADRLAIYVNAVYNNPEQHSGQTLALAT
                     VFFAFQIYCDFAGYSNIAIGAAKVMGFTLMTNFNRPYFSRSISEFWKRWHISLSTWFR
                     DYLYISLGGNRVKIPRMYFNLFLVFVISGLWHGANWTFIIWGALNGFYLIFALFTQKF
                     RERFNHAVFLDRLPRIHSFIQILITFILACFAWIFFRANSTQDSFHIISSVIHLNGPL
                     YIGTPSVLIYAFFAILFLIAVELKKEYFDGKISFFHHKNVWVRNLSYTGVILLILLFG
                     VFDGGQFIYFQF"
     gene            complement(5581..5925)
                     /locus_tag="PKZ74_00465"
     CDS             complement(5581..5925)
                     /locus_tag="PKZ74_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPM91248.1"
                     /translation="MFKEAGLVERYGSGIKRISRALKSEGFPLPIFENFQSGFRVTVF
                     VKRKTLETGSEKDSEKILEIIKKNPAITAKELGNIIGISSRAVEKQIARLKKSGHLRR
                     TGGRKTGHWEVL"
     gene            complement(5880..6200)
                     /locus_tag="PKZ74_00470"
     CDS             complement(5880..6200)
                     /locus_tag="PKZ74_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91249.1"
                     /translation="MKIELLDNILFKGESDKVELKSRFNDETIETIVAFSNSTGGQIL
                     IGVDDKKNIKGLTIGKESIQKLLNEIKSKTAPSIIPAIEAIEVENKQMCSRRPDWWSV
                     MDQA"
     gene            complement(6202..7356)
                     /locus_tag="PKZ74_00475"
     CDS             complement(6202..7356)
                     /locus_tag="PKZ74_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HPM91250.1"
                     /translation="MDKLKEIVNFIRQAFNEPEAFIPLHEPRFIGNEKRYLAECIDST
                     FVSSVGKFVDTFEEVTAAYTGAKRAVVCVNGTEALHMALLLAGVERDEEVITQPLTFI
                     ATANAIAYTGAKPVFVDVDRDTLGMSPASLRNFLEESGEVRNDGFCYNKSTGRKLSAC
                     VPVHIFGHPCRIDEIVEICSRFHIPVVEDAAESIGTLYKGKHTGTFGKLGILSYNGNK
                     TITTGGGGMILTDDEALGKMAKHLTTQAKVPHQWEYVHDHVGYNYRMPNINAAMGVAQ
                     MESLAYFIEKKRELAAAYKSFFDSIGVSFFTEPEHAFSNYWLNAVILGDRKKRDELLE
                     YTNREKVMTRPAWRLMNRLPMFENCRAFHLENAMWLEDRIVNVPSSVVIK"
     gene            complement(7404..7754)
                     /locus_tag="PKZ74_00480"
     CDS             complement(7404..7754)
                     /locus_tag="PKZ74_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM91251.1"
                     /translation="MTRKRDIRIYLEDISESSYLIASYIADLSEGAFYNFPEKQDAVL
                     RRIQIIGEAAKHIPEDKRLKWNHIPWKAIAGMRDILVHEYFGITLSMVWKLAVEDIPV
                     LKQQVDEILNQSDV"
     gene            complement(7751..8041)
                     /locus_tag="PKZ74_00485"
     CDS             complement(7751..8041)
                     /locus_tag="PKZ74_00485"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM91252.1"
                     /translation="MQVDIDHIIKLCKPVFKKYGINSASIVGSMAYGDYSETSDLDVV
                     VEISQSLSLLTFSKIKIELEEVLNRKVDLIVRSTIKERMKKSMLAKEIIIAL"
     gene            complement(8073..9065)
                     /locus_tag="PKZ74_00490"
     CDS             complement(8073..9065)
                     /locus_tag="PKZ74_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HPM91253.1"
                     /translation="MELTNKTVLVTGADGFIGSHLVEALLEKGCNVRALSFYNSFNFW
                     GWLEDIPAYPNLEIITGDIRDPHFIKKIAGDTDIIFNLAALIAIPYSYVAPDSYVDTN
                     IKGTLNICQAALEHGGIRVIHTSTSEVYGTAQYVPIDEKHPKQPQSPYSATKIGADAM
                     ALSFYNAFGLPVTIARPFNTYGPRQSARAIIPTIISQIASGMKQINLGDLRPTRDLNF
                     VKDTCSGFIALAQCDKALGKEINISSNYEISMKDVFGLIREIMNSGVEFVTDEARIRP
                     ENSEVFRLWGDNRLIKELTGWQPEHDIRKGLEITCDWFSRPENLAKYKPGIYNL"
     gene            complement(9075..9770)
                     /locus_tag="PKZ74_00495"
     CDS             complement(9075..9770)
                     /locus_tag="PKZ74_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPM91254.1"
                     /translation="MKAVILAGGLGSRLRPFTQIIPKPLLPIGEKSVLEIQIEHLKKH
                     GFNEIFLATNYKSEYIENFFGDGSRFGIKLKISKEDKPLGTAGPITLLKNELTDPFLM
                     MNGDILTLLDYKNFYDFAVKTGADLTISVKEYITPFQFGNIEFEGDYVTYIKEKPQIR
                     MNVLAGIYIFRPEIFRLIPDNTFFGMDLLIDKMLNEKIPVAKYQIYEYWLDIGQVDDY
                     EKAQEIYKTHFSK"
     gene            complement(9947..11173)
                     /locus_tag="PKZ74_00500"
     CDS             complement(9947..11173)
                     /locus_tag="PKZ74_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91255.1"
                     /translation="MFDQKDVFNHQKHLIDRNIDLEKYIKTRQVVIISGIRRCGKSSL
                     LYLIKEKMKLDEQGYCYFNFDDERIIPDVSILEQIYNQHIETYGNDPVLFFDEIQNIS
                     SWEKFVNRMYEKGTKIFVTGSNAKLLSSQISTSLTGRNKILELFPFSFSEYIRYTGNV
                     YIPGRITPKQKSLLVKDFNSYFEIGGFPLVVKENDLELINAYFQDILYRDIISRYRLS
                     QVNEIKQIGLYFASNTGKLFSYSTLQNISGVKSLSSIKDYLWYYEQSYLFFYLKKFDY
                     SVKKQIMNPKKVYAIDPAVSNRLGFNFSENKGRILENIVYLELLRRGKEIYYHAGKSE
                     CDFLVKEGLKVTEAIQVAWTVDATNLRREIKGLQEAMRTYGLDTGVLIAYDAGDTIDT
                     PDNITIIPAWQWLLEE"
     gene            complement(11345..12520)
                     /locus_tag="PKZ74_00505"
     CDS             complement(11345..12520)
                     /locus_tag="PKZ74_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91256.1"
                     /translation="MFIPRKIYSGLVAHLEKKPITLLTGMRRTGKTTLVKQLLEDCAD
                     KNKLYIDLERIDNRLLFGEKNYENILLALAGRGLDINSKMTIAIDELQLLPEITSVIK
                     YLYDHYPIKFILTGSSSFYLKGLISDSLAGRKKIFELFPLDFGEFLTFKNMPHGQPGI
                     SKVFIESEYHRLKGFYDEFILYGGFPEVVLASDSTDKTDYLADIISSYINIDVKSLSD
                     FRNISMVQNLIKMLALRCSYKLDISKISSLTGISRPTVQSYIELFEKTYLIHLLPVYS
                     QNTDREIVKMKKIYFCDTGLLNVFANPGSGITFENALFLQLRQHSDLKYYSLKTGNEI
                     DFIADKEAYEVKETPAYYDLENLKKTSSRLTLRNHYVVGRFPPAKHFNEFIWGGNIV"
     gene            complement(12667..13479)
                     /locus_tag="PKZ74_00510"
     CDS             complement(12667..13479)
                     /locus_tag="PKZ74_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM91257.1"
                     /translation="MIRLDQIQSFFPPQVRDNPAHQKHILKEYIQLMILDYLSSTSYA
                     RKIVFIGGTNLRLIKGIDRFSEDLDFDCKSFTEDEFNEMTDSVLLYLERQGLRLETKE
                     NINKSLQAFRKNIFFPELLFELGLSGHREERFLIKIECQDQEVPYATQKVNIKGCGFY
                     FPFPVPSDGVLTAMKLSALISRQKGRDFYDAMFLLGQSGPDWAFLTARWGIGDAASLH
                     KALHDLLQKIDLDHKARDFDHLVFEKRHSTRILMFKEFIDTLPLFSSLNGEL"
     gene            complement(13476..14093)
                     /locus_tag="PKZ74_00515"
     CDS             complement(13476..14093)
                     /locus_tag="PKZ74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91258.1"
                     /translation="MSFQEFRIAFYDLACFSTNQVLAWYPGFDKNNLGRWIKKGFLVK
                     LRNSYYCFPEYINKPGFSFYVGNLIYRPSYISLHSALSYYGLIPEAVVQVTSVSSLKT
                     ASFTNSSGTYFYKTLKPELMFGYDLKTYSNSWTVHLSQPEKALLDLLYLNPYYNTQQE
                     MELLRLDGDIISESLDLERLKDYVTRYRNRSLENRVSMLLKAYSL"
     gene            complement(14241..14801)
                     /locus_tag="PKZ74_00520"
     CDS             complement(14241..14801)
                     /locus_tag="PKZ74_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPM91259.1"
                     /translation="MLDTLITSKTRLKLLLKFFLNSGARAHLRNLESEFGESTNAIRL
                     ELNKFEEAGLLKSEIKGNKKVFRANTGHPLFSDIHNILLKTIGFDQIIDRVVTKLGNV
                     EKAYVTGDFARGIDAPIIDLVLVGDDINQEYLVKLANKTEELIKRKIRYIIFRPEEFE
                     DYRRKISAKDLLLIWRSEERGVGSRQ"
     gene            complement(14929..15282)
                     /locus_tag="PKZ74_00525"
     CDS             complement(14929..15282)
                     /locus_tag="PKZ74_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM91260.1"
                     /translation="MNHKDLDVWKEGINFVKMVYQITSRFPDIEKFGLITQIRRAAVS
                     VPANISEGAARQSDKEFIHFLYISLGSIAELETLFIISADQNYLNEADSKEIDNSLQK
                     IRSMLIGLIRYLKNK"
     gene            complement(15567..16064)
                     /locus_tag="PKZ74_00530"
     CDS             complement(15567..16064)
                     /locus_tag="PKZ74_00530"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPM91261.1"
                     /translation="MNWFAVYTNPRAEKKAHADLLEKGIEAYLPLERKLKQWSDRKKW
                     VEEPLFRSYIFVHIPLARYFDVLNTAGIVRYITFEGKAVAVPQNQIDAIRYYLSETET
                     VPEEQDLKDLKPGAAVEIIRGPLRGLTGQLIEFQGRRKVRIEIEALGQFLNLNIDVKD
                     LRIIR"
     gene            complement(16071..17087)
                     /locus_tag="PKZ74_00535"
     CDS             complement(16071..17087)
                     /locus_tag="PKZ74_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM91262.1"
                     /translation="MNFELNNVRLLVTGGAGFIGSALCETFLQQDNEVVCLDNLITGF
                     KRNIEPFLSNPRFTFLEGDIRNPETCRQACEGVQYVFHQAALGSVPRSIENPARTNEI
                     NINGFLNMLIAARDAKVKRFVFASSSSVYGTSPALPKVEEQIGEPLSPYAVTKLVNEK
                     YARVFYDIYGLETIGLRYFNVFGRRQDPFGAYAAVIPKWVAQLMKHERPVINGDGSYS
                     RDFTYIDNVVEANEKAATAPTPESPPPAGGGDFGEGAVFNVAYGANTTLNELFSILRD
                     NLSKYDPEIAKIEPVYGSERAGDIPHSHASIEKARKLLGYNPQFDARKGFELACDWYY
                     KHLR"
     gene            complement(17105..18397)
                     /locus_tag="PKZ74_00540"
     CDS             complement(17105..18397)
                     /locus_tag="PKZ74_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPM91263.1"
                     /translation="MNYFVVEENFSSKAYLEMELLRFTTAGSVDDGKSTLIGRLLYDS
                     KSIFEDQLEAVKRASIRKGNEVINLALLTDGLRAEREQGITIDVAYRYFATPKRKFII
                     ADTPGHVQYTRNMVTGASTANAAIILIDARNGVTEQTYRHSYLASLLQIPHLIICVNK
                     MDLMNYSEEVYEKIKQDFGAFAAKLDVKDIQYVPISALNGDNVVEKMNNMPWYEGGTL
                     RYILENVHVASDQNHIDPRFPVQYVIRPISDKYHDYRGYAGRIAGGVFHKGEKVMVLP
                     SGFITTLESIDMYETELNEAYPPMSVTFRLADDLDISRGDMIVRENNVPEISQDIEVM
                     MCWMNEKPMAPGMKYYLRHTTSEVRAMIKEIIYKVDVNTLERMPDGKELRMNEIGRVQ
                     LRTTKPLYFDSYRRNRTTGSIILIEEGTFNTVGVGMIV"
     gene            complement(18508..19425)
                     /gene="cysD"
                     /locus_tag="PKZ74_00545"
     CDS             complement(18508..19425)
                     /gene="cysD"
                     /locus_tag="PKZ74_00545"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPM91264.1"
                     /translation="MTDHQRKYNLNHLRELESESIYVMREVAAQFERPGLLFSGGKDS
                     IVMVHLAKKAFWPAKLPFPLIHIDTGHNFEETMTFRDELVSKLGAELIVGSVQESIDT
                     GRAVEETGYNASRNLLQTVTLLDTLTRYKIDAAMGGGRRDEEKARAKERFFSHRDDFG
                     QWDPKNQRPELWNIFNGKKRVGEHFRVFPLSNWTEMDIWQYIYLENIDIPNIYFTHER
                     DVFNRDGVWLADAPFMKKKENEKVVRMRIRCRTIGDISCTGLTVSEASTLEDIIAEIA
                     AIRVTERGSRADDLRSESAMEDRKKEGYF"
     gene            complement(19430..20080)
                     /gene="cysC"
                     /locus_tag="PKZ74_00550"
     CDS             complement(19430..20080)
                     /gene="cysC"
                     /locus_tag="PKZ74_00550"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPM91265.1"
                     /translation="MPENIFPVFHKIIQRGDKEKRLGQKAVVLWFTGLSGAGKTTLAK
                     RLEEELFARNYMVQILDGDNIRSGINNNLSFSDDDRRENIRRIAEVSKLFLNCGIIAI
                     NSFISPTQEIRHLARSIIGKDDFIEVYINAPLQVCEKRDVKGLYAKARKGEIKDFTGI
                     SSPFELPEEADLEIRTDLLSIEESVQKLLAFVLPKIQPAVGSQQSAVYDQRPTTND"
     gene            complement(20132..22636)
                     /locus_tag="PKZ74_00555"
     CDS             complement(20132..22636)
                     /locus_tag="PKZ74_00555"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPM91266.1"
                     /translation="MENDNTRIYQQTQPIQQQEEGFDYKALFFKLYRHWYFFILTIVV
                     ALIIAFLFNKYTKPVYEVSTTVIIKSDRGNKIDPQQLIGLGFRNDLQNIENEIGVLTS
                     YSLTERTIRNLDFFVAYFEEENFISKELYGNSPYEVVFDTSHLQPVNVEFKVTILSSS
                     KYTIQAEYENVKLYSYSQFKESDQEYPFFKINDTLYFGEQLENEACSFHIVPNPRVKE
                     SNLAGKKLAFVFKDLKSLVGRYQGLNIEPINKDASILKISLRGFNEAKAVAFLNMLTR
                     EYLAQGLEKKNLIASNTIRFIDEQLMDISDSLNYAEMELQDYRSQNIVMDIDFQSQQA
                     VDYMKTLETQKADLSIKNMYYKSLKKYVETNKDVQQGLIVPSAMGIEDPLTTSLIQEL
                     STLFNERAEALLTSTEKNPTIIAYDLRIENTKNALLENITNIVKNSDLTLKDINSRIN
                     ALTDQMTNIPKTQRELLGITRKFTLSDNIYNFLQQRRAEAQITRASNVSDNEIVDRAR
                     LLDASPVYPKKSLNYIIAIILGLVLPVLYVLGKDYLNDKIVERGDIEKITHLPIIGHV
                     IHSNRLNTIVVAESPKSSIAESFRSIRTNLAFVTKGKEQITVLVTSDMVSTGKTFISI
                     NLASIFALYGKKTVLVGFDLRKPKIYQDFGLSNAEGVSSYLINKSTYEGIIQHSMIDN
                     LDIVMAGPVPPNPSELIASPKTDELFERLKKDYDYIIIDTPPVGLVTDAFLLMKYTDA
                     NLFAVRQNYTLKKVFGSIINDIEKRNIPNIHILINDVKLTKGSYGYGYGYGYGYGYGY
                     GYGYGYGYGYGYGYGYYSDDKEKEKESTMSRIFKKS"
     gene            complement(22645..23430)
                     /locus_tag="PKZ74_00560"
     CDS             complement(22645..23430)
                     /locus_tag="PKZ74_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPM91267.1"
                     /translation="MNWSTFLTGKAVFLPLIVLLALASCVPMKRVRYLQDEHQKTEAK
                     SDFKKPEPNEYRIHMGDNLYIKINSLDEKSNYFDYTGGINNYYSESAIYLNSYTVNDS
                     GYIEFPLIGKVMVDNLTIEEIQKVLQIEVNEYVKNTVVIVKLANFRISLFGEFNNPGK
                     YVIYQDKINIFEALAMAGDMTGFAKKNTILLVRQTEKGLKKHRLNLNDNSILESEFYY
                     LMPNDMIYAEPVKGKPFAFTEFPYAIIFSVITTSLLLYEVITK"
     gene            complement(23518..24693)
                     /locus_tag="PKZ74_00565"
     CDS             complement(23518..24693)
                     /locus_tag="PKZ74_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPM91268.1"
                     /translation="MDLSIYFEPVQLKGYHFSEKTRRKLMGDVVKAYIEPNDFPSLDH
                     VDLAFIGVGEERNCFFNNGCGLGPDAIRKEFYKLYQGNFKLRMVDLGNVRQGHSIEDT
                     YFAVTAIMAELLEHNIVPVILGGSQDITYAQYKAYEKIGRIINIASVDSQFDIGTGQE
                     KLDSRSYMSHIILHQPNYLFNYTNIGFQTYFVDQQAITLMKNLYFDTYRLGIVRNNLE
                     EVEPMVRNADMISVDMGAVRASDAPGNANATPNGFYGEEICQVMRYAGLSDKLTSIGI
                     YELNPRLDRQSLTAQLAGQMIWYFIDGFYSRKGDFPPSKKKELVKFTVNMKDFKDEVV
                     FYRSKKSERWWMEVPIRSKHRERYERHHLVPCSYQDYETACQNALPDRWWQTYQKLM"
     gene            complement(24749..27082)
                     /gene="topA"
                     /locus_tag="PKZ74_00570"
     CDS             complement(24749..27082)
                     /gene="topA"
                     /locus_tag="PKZ74_00570"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPM91269.1"
                     /translation="MIKNLVIVESPAKAKTIERFLGKDFTVRSSFGHVMDLSKKGLGV
                     DIEKDFIPIYEISPEKKKVVGELKKAAKEAEVVWLATDEDREGEAISWHLVSALGLDE
                     KATRRIVFHEITKSAILDAIDNPRQIDRDLVNAQQARRVLDRLVGFELSPLLWKKVKP
                     ALSAGRVQSVAVRLIVEREDEIKAFKETSSFKVTAKFTVTIKGAEYKFEAEFPTRFKT
                     REEARKFLEKCIGASYKVTGIETRPSKKSPAPPFTTSTLQQEASRKLGFSVANTMRVA
                     QQLYEEGLITYMRTDSVTLSKMALAMAKEEIIKLYGPEYSKTRQFATKTKGAQEAHEA
                     IRPTYIANAAISGSATQKKLYELIWKRTIASQMSDALTEKTIVNISASTLGGEEFIAQ
                     GEVIKFDGFLKVYRESFDDDSDEEGRSILPPLSVGDQPGMLEMNAEQRFTQPQRRFTE
                     ASLVKRLEELGIGRPSTYAPTISTIQKREYVEKGDVEGFIRTYDLLTLKNNEIKLHEK
                     KDKAGNEKGKLLPTDLGTLVNRFLMQYFQDIIDYQFTATVEKDFDEIAEGKKEWNEMI
                     RKFYKPFHSQIRDTVESTGKFSGERLLGIDPGSGKNVYVKLGRFGPVVQIGETESDEK
                     PRFAGLGKGQSLDTLTLEQALELFKFPRNLGLYENAEMIVAVGRFGPYIKHNNLYYSL
                     TKQDDPALIDQERAISLIELKRKQDREKIIKSFPENENVVVMNGRFGPYIAISKVNYK
                     IPKGTDPASLSLDECLQIAEKQEKSGKKKTIPTRKKS"
     gene            complement(27284..27955)
                     /locus_tag="PKZ74_00575"
     CDS             complement(27284..27955)
                     /locus_tag="PKZ74_00575"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HPM91270.1"
                     /translation="MSNILMDPIGRLMGLRYKSHPWHGVDIGREAPVIVTAFIEMVPT
                     DTVKYEIDKESGYLRIDRPQKYSNVVPALYGFVPQTFSSTAVAEFCMQKSGRTGIKGD
                     SDPIDICVLTEKEISHGNILVKARPVGGFRMIDRDESDDKIIAVLNNDTVYGHINDIA
                     ELPVLVVDRLKHYFLTYKDLPGKESNVVITHVFGIDEAHEIIRRSMEDYHHKFDTLER
                     ALSNV"
     gene            complement(28018..28575)
                     /gene="ruvC"
                     /locus_tag="PKZ74_00580"
     CDS             complement(28018..28575)
                     /gene="ruvC"
                     /locus_tag="PKZ74_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPM91271.1"
                     /translation="MSSERVILGIDPGTVIMGYGVVLVRDKQIELLTMGVLKLHRYSE
                     HEDKLKRIFERTIALIEEYHPDELAIEAPFFGKNVQSMLKLGRAQGVAMAAALSRTIP
                     IFEYSPRKIKQAITGKGSASKEQVALMLQHLLKFSESPEYFDASDGLAAAVCHYFQNN
                     PGGGKKEKKFTGWKAFLAENPGRKA"
     gene            28591..29610
                     /locus_tag="PKZ74_00585"
     CDS             28591..29610
                     /locus_tag="PKZ74_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM91272.1"
                     /translation="MTEQSAKIRKYVNFLLRAVIIILTYGFIYHQIFLENKLDDLSDV
                     FLRLAERQDVKWILALMMLLMFINWGIESFKWKLLISRIEHVSFFRSFKAVMAGISVS
                     LFMPNRTGDYLGRVFILEKGNHVEGIFSTIIGSFAQMIITFGIGLLCLLTFTDHYFRE
                     SLNIGEYMFMSLVFLVPVMVFALIMIYLKVGVLAEFLSRFLPGRWPRLKENAAIFSRY
                     SAGELSRVLLLSFLRYIVFSTQFYILIRVFGAPVPAAEGFVLIPVIYLAMALVPTFAL
                     VELGVRGTVSLFIFGLYFEKSGIVDPQILLAPLAASSLLWLINLVLPAVIGTFFVFSL
                     KFFRK"
     gene            29619..30749
                     /locus_tag="PKZ74_00590"
     CDS             29619..30749
                     /locus_tag="PKZ74_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM91273.1"
                     /translation="MATGFLMILILALLIALSYLVLIAIFTFGWYRKNGEKNFSGFSL
                     MKISVVVAVRNEESNMAGLLNDLIRQDYPGGLLEIIIVDDHSTDRTAGVTRNFIQDHP
                     LSAIIFILNNDEKKRGKKASVELGISRATGEVIVTTDADCRMREGWIRALAGRFTKEH
                     VQMVFGPVTIGKQDNFAGLFQAMEFDGLVASGGGAALAGYPFLCNGANLAYRREAFHT
                     TGGFSDNMRYISGDDVFLLHKIKKQFGAGAIVFCKDEQAIVDTAPVSGAGAFLRQRIR
                     WASKSKGYRDSLSIITAITIFSFSLTVLLSFLAGFTDPMYFSISGGLFLLKIFADLPL
                     LAGITKFTGKPFPFMWYPVFQLIYPLYIIIAGILSLSNRNKW"
     gene            complement(30771..31820)
                     /gene="queA"
                     /locus_tag="PKZ74_00595"
     CDS             complement(30771..31820)
                     /gene="queA"
                     /locus_tag="PKZ74_00595"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPM91274.1"
                     /translation="MKLSQFKFYLPSELIADRPPENRDDSRMMVLNRKTRTIEHKSFK
                     DILGYFSDGDVFVINNTKVFPARLYGTKEKTGAKIEVFLLRELNRESRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPYEDFKKTITDLGQTPLPKIH
                     KRTVEPLDDIRYQTIYAKHEGAVAAPTAGMHFSRELMKRLELLGVNYAEITLHVGLGN
                     FRTIEVEDLTKHKMDSEEMLIEETAAGIINSAKENRKKIVSVGTTTMKALETAVSISG
                     KVKPYRGWTNKFIFPPYDFTVADALVTNFHLPMSSMMMMAAAFSGYDFLMEAYREAID
                     QKYRFFTYGDAMLII"
     gene            complement(31933..32616)
                     /gene="truB"
                     /locus_tag="PKZ74_00600"
     CDS             complement(31933..32616)
                     /gene="truB"
                     /locus_tag="PKZ74_00600"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPM91275.1"
                     /translation="MFDFHKGELLLIDKPLDWTSFDAVNKIRRLIEKKTGVKLKVGHA
                     GTLDPKATGLLLICTGAMTKQIVNLTGLDKEYCGTFILGATTPCYDTEKPVDRTFPVD
                     HITEEMIRDTAKRFTGTIQQVPPQFSAKLVDGKRAYLSARKSREVRLDPVTITISEFE
                     ITHIALPEVGFRVSGSKGTYIRALARDFGEALGSGAYLASLRRTRIGDYRVDDAINLP
                     QFEVLVAQQ"
     gene            complement(32616..33422)
                     /locus_tag="PKZ74_00605"
     CDS             complement(32616..33422)
                     /locus_tag="PKZ74_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPM91276.1"
                     /translation="MNWFEALILGLVQGLTEFLPVSSSGHLELGKAILGVDPERSLVF
                     TVVVHGATVLSTIVVFRKDLWQLIREVFAIRWKDAVALRFTETMQYNFKLILSMIPVV
                     ILGLFFMEEIESLFTGRVVFVGFMLIVTALLLASTYLIKNNNRKITFADSLIIGIAQA
                     IAVIPGISRSGATISTGLLLGNKRELVTKFSFLMVLIPIIGANAKDLAGSEMMTETTV
                     SATSLLIGFAAAFISGFFACKWMIGIVKKGNLVYFSAYCFIIGLIAIIFG"
     gene            complement(33444..33734)
                     /locus_tag="PKZ74_00610"
     CDS             complement(33444..33734)
                     /locus_tag="PKZ74_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPM91277.1"
                     /translation="MVTKTKPVAKEKASPLKAPEPESRAGFAFGRENYRLLFIALGLI
                     VLGFLLMIGGGSKDPNTFSYGIFNFQRLTLAPILILAGYVVGIFSIMKKPKD"
     gene            complement(33773..34651)
                     /locus_tag="PKZ74_00615"
     CDS             complement(33773..34651)
                     /locus_tag="PKZ74_00615"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPM91278.1"
                     /translation="MSKMEERYHRRRYQSSVATTIVSITLVLTMLGLLGLTILHARKL
                     SDYVKENIGFRVYLKEDAATEDIIILQKKLDAEPFVKSSRYISPEEAARELTADLGED
                     FIDFLGYNPLPPSIDLRVRAAYANVESLETIERNLMQEVVVKEVFYQKSLVHLINKNV
                     NRISIVLLGFTGLLMLIAIALINNTIRLSVYSKRFIIRTMKLVGATRRFISRPFIVRG
                     VLQGFYSAIFAIILLVTILYFLMQQVPELVNLFDLYLYLAVFLLVMLTGMFLAWVSTW
                     FAVRKYLRMTEDELYS"
     gene            34892..37741
                     /gene="leuS"
                     /locus_tag="PKZ74_00620"
     CDS             34892..37741
                     /gene="leuS"
                     /locus_tag="PKZ74_00620"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPM91279.1"
                     /translation="MEYNFNAIEKKWQDYWRENKTFKAFNQSGKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLQGFNVLHPMGFDAYGLPAEQYAIQTGTHPAITTEK
                     NIARYREQLDQIGFSYDWDREVVTSDPFYYKWTQWTFIRLFKSWYNRESNKAEQIDKL
                     VNIFEQEGNANVKAVCNPTDTFTAESWKAMTEKEQQQILMNYRLAYLSDTWVNWCPAL
                     GTVLANDEVKDGFSERGGHPVERKQMKQWSLRITAYAQRLLDGLEKLEWSDSLKEVQR
                     NWIGRSEGAAVYFQLAAGSWQPAAGSWQSNMRPGTRNPEPGTFIEVFTTRPDTLFGAT
                     FMVLAPEHELVPQITTPDRKEKVAEYVEWAKNRSERDRMTEVKRITGEFTGAYGINPL
                     TGEQIPIWVADYVLAGYGTGAIMAVPAHDSRDFAFARHFDLPIIQVVKKEDEEESDPM
                     LWSESYDAKDGVMVNSGFITGLKVKAAITATIRKIEEQGVGYGTVNFRLRDAIFSRQR
                     YWGEPFPIYYREGMPYPLDESRLPLELPEVDKYLPTETGEPPLARAKNWHTPEGYPLE
                     TSTMPGFAGSSGYYLRYMDPWNNDAYFSKESNRYWQDIDLYVGGDEHAAGHLIYSRFW
                     NMFLFDIGLVCKEEPFKRLINQGKIQGRSSFVYRINGTSKFVSYGLKDQYDTTRLHVD
                     IEMVDNDVLDIEAFRNWRAEYRHAEFILENGKYICGWEIEKMSKSKHNVQNPDDLIVR
                     YGADTLRMYEMFLGPVELSKPWDTKGIEGVFRFLKKYWRLFFTEQGEFQVTDETPTAA
                     ELKILHKTILKTKEDIERFSFNTTVSSFMICVNELTDLKCNKRSILEPLNILISSFTP
                     HIAEEIWSLLGHDGTIAYASFPEFDEEFIRENTFNYPVSFNGKLRFQLELPVDFAPAD
                     VEAAVMAAPEARKWLAGGAPKKVIFVPKKIINVVV"
     gene            37738..38745
                     /locus_tag="PKZ74_00625"
     CDS             37738..38745
                     /locus_tag="PKZ74_00625"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPM91280.1"
                     /translation="MSSRFQIIIRPVGKVFHKSNQPVLLVVRVFQKSVISNDGLTLFL
                     APTTKAVGSYGFMKVGTDGFSRRTTKKSLGLRLSLGFLFLFISNQLISQDTFRKVENN
                     TFQHGEFLKYRVFYDSWVTYWMTAGYGTMEISPEPVKINGRDTYHITVNGNSAGLFNV
                     FYKVRDKFETYMDKEGLMPLKFIRYTREGGYKKDDTIFFDHQARKAVSIRKTKDITQY
                     VQDIVSAFYYVRSWDFDTAEVNDTYYVDFFLDDSLYHSEIVFMGRESVRTDFGTLPCM
                     RFKPRVAVGELFQDPYPMELWVTDDRNKIPVLMKSAVFIGSVKIELVEYRGLRWPFES
                     H"
     gene            38964..44987
                     /locus_tag="PKZ74_00630"
     CDS             38964..44987
                     /locus_tag="PKZ74_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPM91281.1"
                     /translation="MNKSILLFLIIAISCTTMGQKSYEKEWKEVEAAVRKGLPQSALK
                     IVDSVYLDARSSDNAPQFLKATLYQIKLRSDFQENFMESSILQVETELKTASVPVKQI
                     LHSIRAELYWRYYQGNRYRFMERTGVSNPDLTDIKTWDLKTLVNTVTESYLASVGQEY
                     ATLKIKLDFIDPILETEPGSKIYRPMLYDFLAHRALEYFANEEPAVVRPAEGFLLDQE
                     NYFSPAGEFSSLKITSPDENDLKWRALLIYQNLLRFHSGDDDPSALIDADLARLKFVQ
                     QHSVLQDKDLLYKNALAELQEKHKSAPGAADVAYELAASYNLSGTFYVRNQGEVNRWD
                     LKKAREICEKTIRDYPGTDGAKNCAILLAEISEPSLNFSVNYANVPDQPFLGSLNFRN
                     VSTVYFRIVKADPEEDRNRRQREMGENLVAKYLALQPVKAWSVKLPDDGDFQTHNTQV
                     RIPSLPKGYYVILASDNEGFSYENRPVAYSPAWVTGISYVSQRNPEDGKLEIYVLDRD
                     KGIPMKGVSVAAYIREYDYKTRSYLDKLINTLTSDENGYISMAAVRNDSKSFFLKFSQ
                     GNDLYFTENYFYLYPTGPTEDTRITTYFFTDRSIYRPGQTVYFKGIVLERTGTKSEIK
                     PGYSHVVTFYDVNNQKVSEKAVTTSDFGSFNGIFTAPAGGLTGEMSIRCETGSVGFSV
                     EEYKRPRFKVEVDPLEGSYKLNETVNVTGKALNYSGSAVDQASVSYRVVRTARFPVWR
                     SWWRWFPPTPETEITSGKTLTGADGSFSLSFKALPDLTVDRKYQPVFNYTVYVDVTDI
                     TGEVRSAEGSLSVGYQAMLLDAGIPDEVDQLGPDAFPLSATNLNRQPVPAEVKVSVHL
                     LDAPARLIRKQQWENPDVFTMTREEFLASFPYELYDDENDPETWNKKELMMEKSYRTP
                     SDSTIIINNLKSWKEGHYVLTIESKDAYGEKVETKKFFTLFNPSLKSLPSNDSFWYSV
                     IKPGGEPGDTVAFTAGTAEQGVRLLYEIENDGKIISREWISLSREKRTFRIPVKEEYR
                     GNFFVNLVFVKGNRSYGVSEQFTVPFTDKQLKITAETFRDKLTPGQEEEWKLRITGMN
                     GDKVAAELLASMYDASLDAFRGHSWSFGLYPVRSSAAGWSVFDAFTQASSHLVKKQPA
                     DPPSYIFHSYDRLNWFGFNYYGGIYPRMGGMEMRMKNAMPAMDGMAADKAQEEAEMTV
                     TESGNIPAEPLPDEKKPEVPDMPVRKNFNETAFFFPDLKTDEKGDVILSFTVPESLTA
                     WKLMALAYTKDLKTGYLEKEVATRKELMVMTNAPRFFREGDQVMFAAKLVSMSDKKLA
                     GKIQAEFFDAYTMIPVDALVKNLVKVVDFSVEKGKSEVFFWDIVIPEGLDALVCRVKA
                     SADEFSDGEEIVVPVLPNRMLVTETLPLPVNGKGTKNFKFSKLASSGKSGSLSSYRLT
                     LEFTSNPAWYAVQALPYMTGYVHESADGLFTRYYANSLASYIANSNPKIKAVFESWKN
                     ITPDALLSNLEKNQELKAVLLNETPWVMEAKSESERKKRIAVLFDLNRMAAEQQSALA
                     KLQQMQTPNGGWAWFKGMRDDRYVTQLIVTGIGRLQHLGVIELQRNPDLISMTQSAFR
                     YLCDRLKEDYDDIKKDSKGRMDENHLGSAQIQYLYAYSYLMEFVKVHPSNKEAFDYFT
                     SQAGKYWKDQNKYLQGMMAIALSRMNVPDVPAAILASLKENALVSEEMGMYWKVPEGY
                     FWYEAPVERQAMLIEAFAEVTRDEEAVELMKIWLLKQKQTQDWKTSRATADAVYALLL
                     KGTDLLASDQLVEVTLGTEKIDPLTMDGVTVQAGTGYFQVSKMGKEITPEMGNIKVVK
                     KDEGIAWGAVYWQYFEHLDKITPAASPLSIERELYLERNTSTGPVLDPVFDNSILKTG
                     DRVKARIIIRVDRDMEYVHMKDMRAAAFEPIEAISGYRWQDGLGYYESIRDASVNFYF
                     DYLRKGTYVFEYPLNVTQKGEFSNGITSIQCLYAPEFAAHSQGIRVKVE"
     gene            45317..46024
                     /locus_tag="PKZ74_00635"
     CDS             45317..46024
                     /locus_tag="PKZ74_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91282.1"
                     /translation="MTGDEIPGLINVTDFGHPLKQPYEAFTSNSAHVSKNGKWVIISS
                     HIIIDDFSDDENESELEAYAECLQVFDSNGNLFRKIFSDDAEMNDVGITDDGDYLFYD
                     YQILKDLYYDNHSNRNPGLRIINLKTMDIELDISDYDTNWVYLYPYVAGDFICIFSET
                     RKDYEDVDSTGCNSAVCYNFKKNIKYTGPIGPAFKEFWRIIDVSDEGIYFRVSSAREA
                     QPVLYRFDKDFTKEKIK"
     gene            46201..47418
                     /locus_tag="PKZ74_00640"
     CDS             46201..47418
                     /locus_tag="PKZ74_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91283.1"
                     /translation="MILKETIVEILHQQDEKIKAKDAGIPRQLNDNIAVGGNLILIIS
                     GIRRCGKSTLLNQIKTTTQKSCYFNFEDILATDFELSDFKKLDSIIKELYPGAYLFFD
                     EIQVVEGWENYARSAQDDGFSIIITGSNASLLSRELGTKLTGRHLTYELFPFSYAEFL
                     VYKKASPGIDSFSDYFITGGFPEYIKHSNPEILQQLLRDILNRDIIVRHGLKNSTLVG
                     KLATFLMTNAGKKFSYNSLMKTFETGSANTIIDYVSYFEDSYLLLTIPSFSYSIKDQI
                     RNPKKIYSIDHGMARVNSLSFSKDEGRILENMVFLHLRRKWKKIYYYSVKSECDFVVF
                     DRDKVEMVVQVCYELSDDNMAREISGLKEAMDFFQLKTGFLISYNQEDVIEDGERKII
                     VMPAWKWMMERKL"
     gene            47415..48425
                     /locus_tag="PKZ74_00645"
     CDS             47415..48425
                     /locus_tag="PKZ74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM91284.1"
                     /translation="MNPKHYTIPIFIPQLACPFQCVFCDQEKITARGHIPGIPEMVNI
                     IEQYHFSFPKGKKHVEIGFFGGTFTGIPLEEQEKYLQVAGEYIEKGWVQGIRLSTRPD
                     YIDETRLHLLKKYHVTTIELGAQSMDDEVLALSSRGHTAEDIEMASQMIIKSGFRLGL
                     QMMIGLPGDTLEKDVSTAEKIIALGAVETRIYPLLVIKDTVLEKWYHQGKYQPLTLEE
                     TIDRLKILLPMFEVSGVEVTRVGLHASIGLQTGKELVAGPYHPSIRELAMTGVWWDRL
                     EVLTAFEGTDELRITVHPSQYNFAVGYYGKNRKRLMQVIKEVKFLKDKDLNQMEFLVD
                     YR"
     gene            48481..49218
                     /locus_tag="PKZ74_00650"
     CDS             48481..49218
                     /locus_tag="PKZ74_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91285.1"
                     /translation="MLIIVDNKLSCGAKNILSSYGELMEIQTEGITYPAISGHPDIFF
                     CQGPGKLIVAPNLPERYFDQLRKNGIEFITGELPVGPEYPSSARYNAVATPKYLIHNF
                     RHTDVMITRTFMELEPVCVEQGYCRCNLLALKDDCFMTSDEGIEKVFMRWSLGRRRLH
                     LTNRGRHPLGPRPQGRHPYGILLEGFPHGFFGGCCGVLDDKIFINGNLDFFHEGKKVR
                     DFISSLNYEIVELHHGPLTDAGSILFC"
     gene            49400..51148
                     /gene="aspS"
                     /locus_tag="PKZ74_00655"
     CDS             49400..51148
                     /gene="aspS"
                     /locus_tag="PKZ74_00655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPM91286.1"
                     /translation="MLRSHTCGELRLTDNGKTVTLTGWVQRSRDLGGMTFIDLRDRYG
                     LTQLAFNLETNAELCLQARKLGREFVILATGRVIERSSKNLKIPTGEIEIAVEKLEVL
                     NISKVPPFTIEDETDGGEELRMRYRYLDLRRNPLRRNLELRHRMAIETRNYMDSQGFL
                     EIETPYLIKSTPEGARDFVVPSRVHNGKFYALPQSPQTFKQILMVAGYDRYYQIVRCF
                     RDEDLRADRQPEFTQIDCEMAFITQEDILNTFEGLAQHLFRRVKNYETGPFTRMTFAD
                     AMKFYGSDKPDIRFEMKFAELNDLVTGKGFGVFDQAELTVGICAPGCAEYSRKQLDEL
                     TEFVKRPQIGARGLVYIQCKADGTFKSTIDKFYSPEDLKKIAEAFHAGPNDLILILAG
                     DAHKTRKALNELRLEMGTRLGLRDPNVFKPLWVIDFPLLEWDEETKRFYAMHHPFTSP
                     KPEDIPLLETDPGQVRANAYDLVINGVEIGGGSIRIFNKELQKKMFQILGFSDEDAQE
                     QFGFLMNAFEYGAPPHGGIAFGFDRWVAMFSSVDSIREVIAFPKNNAGQDMMIDSPSE
                     ISGDQLKELGITTVGK"
     gene            complement(51180..52607)
                     /locus_tag="PKZ74_00660"
     CDS             complement(51180..52607)
                     /locus_tag="PKZ74_00660"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91287.1"
                     /translation="MANWPGYGYSQDYSLRISTFEDEFPAGSLSDLNNGIYSIVVRGQ
                     FELDPTPADFFHNYRNTIYHINDQGEIIDDIEIDKIDGYDLLLKSIIRTGDSLMVWGN
                     AYRPEDESYSLVILYFNSNMQLLGINIPYTSDSIIEFFCYTAVDQDNLVFSACKGEYG
                     WDQLLVKTSRTGNFIQERLNPGFAGPWPNIGYLPSVDQILCGRYHWLGYIDNTDFTID
                     SVYYPLSIDLLSYGFFRPYDSSHCIIPSQFYNWDATTDPGYFVIDTIAQATDTALFDL
                     RPDQNEAIDIDYLTIDTMFLGAIENSYMPEPNKGFNPVDRWFAVIKFSKDGEIIWENR
                     FGGEANYILYDITAMQDGSCVMLGTYYDWRNNTMMERDVMIIKSTPDGLYTGLAGHQS
                     PEVQIFPNPANERLFVRISGNSTSSYVIDEITVSDMTGKIWSTSGVNHEKEIMVDVSG
                     YPPGVYYISLFSGNQFLLGKKTVIF"
     gene            complement(52652..>52897)
                     /locus_tag="PKZ74_00665"
     CDS             complement(52652..>52897)
                     /locus_tag="PKZ74_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91288.1"
                     /translation="DYRFWGGTSGLEMLNTSICNQYLLSIKEVVDQYNPYPNPDFILG
                     TLIMNFFEYPYGYWHGCDIWHTADFLYFEVVYVGFPN"
BASE COUNT        13953 a        12281 c        12180 g        14483 t
ORIGIN      
        1 tccgataacc ggcaatacac cgtaaacgga aatatgttta agaaagtctt tcaaaataaa
       61 taagtggtgc gggtaaggat attaatccat ggagggaact taaataatca gaatctatat
      121 ttaagtaaca tatttctata ttgttctact ttttctggaa atttattttc ccattgcctt
      181 acaacttcat tatatacttt ttcatttact actctttttc caatatgaaa tgggatggtt
      241 atcccggaaa aattcctttt aaattttaat aacgaatcat cctcggaatt ggaacgcccc
      301 cctcctaaat gaaaaagttt tgcacccatt tcttgtgcaa attttacagc actgtccaat
      361 aaaaagttat ttacagcatt atctgcattt tcatttctgc ctgagagatg gtagtggaaa
      421 taatcattgt aatgcaagaa aatggaggaa cagatgattt cgccagtttg atttaagatg
      481 ttgaacaggt agattttatc tttcaaatat ttgaacatgt tgttgaagaa atctttgtta
      541 aaataatagt atggttcagc accagcctgt ctcatactat aatgataagt agagatgaac
      601 tggtcaatat gatctttacc tggcgaagtt tcaatctggc aaatatagcc caaccgaata
      661 gctttacgaa tcatattcct gtttttcgaa gaataatcat catcccaaat gtcagcatat
      721 ggttttttca aatcaatcat tacggtttcc cgatctctga ttgtgctcat atcattgagg
      781 gaaaagagtt cattttgcag gatgggatga aatcgggtga attctgcgat tatattttct
      841 tgaatacaga aagaattgaa agagttatag aaatctgaaa tgaatgctgg atcattggaa
      901 gaacttaaga caccattata tccatatgca ccttgtatgt caaaataagt attattcaac
      961 tcaaaattta aattgttgat aggattaatt aaaaatggat aaagtgcaag gttctcatct
     1021 ttttcaaaaa gaaaacaaag ggctttccca tcgccaagat cttcatatag ttgataatac
     1081 cctggggtgt aataaatatc ttgttgttta tttggcaacc tgataagata attaccccaa
     1141 atttcggaat ttgataagtc aagtattctg aaatacataa caaaatggac ttaattaaat
     1201 acctaaagac ccaattaact atcccaatag ttagtctttg caactaattc catgtaatcg
     1261 tttctatgaa cacctacaat tattttatcc caaaatctat tttttctcca gtaccagtct
     1321 cttaacacgc cttccttttg ccagccacac ttatcgatat aaagtttaag agaatgaatg
     1381 ttatattcaa tcatagatcc atctaatcat tgcaacccaa gctcttcaaa tgcatatctc
     1441 atgatagtaa aaaccacatc cacaccaatg ccttttcctc ttatatcttt atccccaatt
     1501 agcataccat gaaaggcatt tctgtctttc cagtttattt cgacaagatt ggtagtgcca
     1561 accaaaccga gatcaacatg gtcgatagca aagcgctgat ttaaattatc agaattgatc
     1621 ctatcaagcc atttttccat tgtatccatg gaagttggaa agtgccaacc ccccaaccaa
     1681 tattgaattt cctgatcatt tgaccatctt tgaaggtcta taagatcatt tttttcaatc
     1741 gcccgtaggg taataatttt tcctttagga ttcatagatt atagttttta aaatttggta
     1801 ttttgaaatt aaaccttaac agatagttct aaacatagtt ttttgaaata ttgctcaatc
     1861 tttaaagctt tccagcattt ttttaatcat aaaatcaaat ttattatcat tcatattcaa
     1921 taatgggaaa gttagaatac tttttgacag cgcaatgctt tcactaaata attgatcatc
     1981 aatctcatca gagagtatcc agtgaactgg agggtagacg ttttctaaga aaagtttttt
     2041 tcttagaaga tctctttccg gaacaacaat tacaaatcca aggggtacaa aacgctgatt
     2101 attaagcaat tccggcacat aattaccaaa attatcaagc agaaactggt aatattgttt
     2161 ctgagttttt attagcagat caatgtcaaa cttttttaaa atagcacggc ttgttttaga
     2221 cattttataa ttctgttcat cgtaataatt tttatccgct gttttgaaaa atttcaaaaa
     2281 tgatgagctt gaaaacattc cgtaattaac atggaaatat cttaatagct ggcccattct
     2341 tttattcaat acaaattttg aacaggttct tttgctaaaa ttgatattta tttctttttt
     2401 cgataataaa acacttccct caatgggaaa aaatttcctg aaactattga aaatataatc
     2461 gccaaacatt ttatcctgat ctaaatgaag gcattgtacc atatcctgaa taatgaaaat
     2521 gtttttggaa cttaaaaaat caattgttgt tgaaagtctg tctttttgag ttgcgccaaa
     2581 gtagtcgata aagagtacgg cttttacatt tttgtttatt agtgaaagca aatggcttat
     2641 atcaacataa aggttttggt caactctgta gaaattgtat ttaatatttc ttgctttaaa
     2701 aggctgaagg atactttgac ataaatagga aggtaaaagc acttcagact cttcatcgat
     2761 atcagacttc aaatgatcaa gaattgctaa taaggaatag tacccgccaa aagtataatt
     2821 tataaaataa tgcttttcta aatcgcttat tagattatca aaagaattat ttgatggttt
     2881 aaaaagcaga cccggagaaa aagtatgttc cccaccaata tacattctaa tttaatttga
     2941 tattcatttg tgttaatatg aatttgttaa ctttaagttg aactctctga acatggttat
     3001 taattccaat atagaatgaa agaatttata ttcattttag agaatattag cctcattatt
     3061 agttttcttc tattcacttt tcacctctta ataaatgttc aatcagtaaa gttgaaaaca
     3121 atgtagcccc agattcatta agatgttcag gatctgtaaa ataatgatga ttattcaaaa
     3181 agattgtatc agatgtaaaa tctagatact ccacattatt ttttaatgct atttgcatga
     3241 caggatttcc tccagtagta tcaactgatt taataaataa cggcgaaata actaatacta
     3301 gcctaatccc atgaagtttg caatcctgga taatctccag taatgcattt tttttatttt
     3361 gatcaattgt tttcactacc atggaatcaa gttctgggta agggttatcc caaatattgt
     3421 ttaatggcac ataaccttta atatcaggtt tacgagcctt gttcaattcc gtattcccaa
     3481 tggcaatagt caataagagt gaattatatg ggtatatttt tgacagacat tttatctttt
     3541 catatggccc cctcaatgct atagtttgat ctatagcttt atttcggttt aaataaggga
     3601 gcaatgatga taatctttca taatcatcaa tcgtagaact caattcatcc ttttcaacat
     3661 caataatcac aatctctggt ttatacctgt taatgactgt tttgaaaatg gcataattat
     3721 aaagtaggaa attcccgtca cgcccggtat tgtaacagct cattccggtc atattttcta
     3781 ttatacttgg gttataatga tggttggctc gggaagaacc aaaaatcaga acctgagcat
     3841 ttgtgctatc catagaatat gttgttctat attgagcgcc tgaagtttgg gtaaaatagt
     3901 aatgttttag ggttgtgcct attgcataat caaataaaaa agtaattcca agaaggagca
     3961 aaattttaca tagaaacttg attatttgat gcttaaaaaa catttaagaa tctaaaattg
     4021 aaaataaata aactgacccc catcaaatac tccaaagagc aggattaata aaatcacacc
     4081 agtatagctg agatttctga cccaaacatt cttatgatgg aaaaaggaaa tttttccatc
     4141 gaaatattct ttctttaatt caactgcaat tagaaaaaga atagcaaaaa aagcatatat
     4201 caaaacagaa ggggttccga tatatagtgg cccattaaga tgaataacac tacttatgat
     4261 gtgaaatgaa tcttgtgtag aatttgccct gaaaaagatc cacgcaaaac aagccaggat
     4321 aaacgtgatt agaatctgga tgaatgaatg tattcgtggt aatcgatcga gaaaaactgc
     4381 atgattgaaa cgctctctga atttttgtgt aaagagggca aagattaagt aaaatccatt
     4441 cagggctccc cagatgatga aggtccagtt tgctccatgc cagagtccgc ttattacaaa
     4501 gaccaggaaa aggttaaaat acattcgcgg gatcttgacc cggtttccgc cgagggaaat
     4561 gtataaataa tctctgaacc aggtagatag ggaaatatgc cagcgtttcc agaattcgga
     4621 aatggaacgg gagaaataag gcctgttaaa gtttgtcatc aatgtgaatc ccatcacttt
     4681 agctgcacca atggcaatat tggagtaacc tgcaaaatca caataaatct ggaaagcaaa
     4741 aaaaacagta gctaatgcaa gtgtctgacc agaatgttgt tccggattat tatagactgc
     4801 attaacgtaa atagcaagtc tgtcagcaat tacgagtttc ataaacagcc cccacaacat
     4861 cagtttcaaa ccctcaacca ctctttggta atcgaaataa tgcttttcat aaaattgatg
     4921 gatcaggttt tgcggacgtt cgataggtcc ggcaaccagc tgtggataaa acatcacgta
     4981 taaagcataa atgccaaaat tccgctcagc tttctgatgt ccacggtaca cttcaatcgt
     5041 gtaactcatg gcctgaaatg tatgaaaaga taatccaatc ggcaggagaa tgctgagatt
     5101 gggtatagaa ttttgatatc caaaagaatt tagaagtgca gataagtttt catttaaaaa
     5161 attgaaatac ttaaatacgg ccaacacacc aatatttgca atcaaactgg caatcagaaa
     5221 atatttcttc ttctttcctt cagtcttttc aagccagatt cctgcaaaat aatcgatgac
     5281 gatggtaaaa ccaagaatga gaatataaat tggaatgaat gccatgtaaa agtagcagct
     5341 cgcagcaagc agcaggaacc acctgaaccg atgcggcagg acaaaataaa gggttgtcac
     5401 taccacgaag aaaaacacga aatgtatgga gttgaatagc attaaagtgc taaagggtta
     5461 aactgttcca gggttccaat gttccagggt tccagggttc cagtgttcca aaattctgta
     5521 ttctgtattt tgtattctga cttctgtctt ctgactgacg aacattactt ttttctttta
     5581 ttataacact tcccaatgac cggtttttct tccacccgtt ctgcggagat gacctgattt
     5641 tttcagcctg gcaatttgtt tttccaccgc ccgtgatgat attccaataa tattacctag
     5701 ttcttttgcc gttatagctg gatttttttt aatgatttcg agaatctttt ccgaatcttt
     5761 ttccgaaccc gtttccaggg tttttctttt tacaaacacg gtaaccctga agcctgattg
     5821 aaaattttca aaaattggta atggaaaccc ttctgatttc aaagcccggg agattctttt
     5881 tatgcctgat ccataacgct ccaccaatcc ggcctccttg aacacatttg cttattttca
     5941 acttcaattg cttcaatagc gggaattata gaaggtgcag tctttgattt aatttcattt
     6001 aataactttt gaatggattc tttaccgatg gtcaatcctt tgatattttt cttatcatct
     6061 acacctataa ggatctgtcc cccagtagag ttcgaaaaag caacaatcgt ttcaatcgtt
     6121 tcatcattga accgactttt taactctact ttgtcgcttt ctcccttaaa gagaatatta
     6181 tcaagcagtt ctattttcat gtcactttat caccacgcta ctcggcacat tcacaatccg
     6241 atcttccagc cacatcgcgt tttcaagatg aaaagcccgg caattttcga acatcggcag
     6301 gcggttcatc agcctccatg ccgggcgggt cattactttt tcgcggttgg tgtattccag
     6361 taactcgtcg cgctttttcc ggtcgcccag gattaccgca ttcaaccagt aatttgagaa
     6421 agcgtgttcc ggttcggtga aaaaagaaac accaatggaa tcgaaaaacg atttgtaggc
     6481 tgcagccagt tcccgtttct tttcaatgaa ataagcaagc gattccatct gtgccacacc
     6541 catggcggca ttgatattag gcatccggta attgtacccg acatggtcgt gcacgtattc
     6601 ccactggtgc ggcaccttgg cctgggtggt caggtgcttg gccatcttac ccagcgcctc
     6661 atcatcggtc aggatcatac ccccgccgcc ggtggtgatt gtcttgttgc cgttgtagct
     6721 tagtataccc agcttgccga aagtaccggt atgcttgcct ttgtagaggg tgccgatact
     6781 ttccgccgca tcttcaacaa ccgggatatg aaagcggctg catatttcaa cgatttcgtc
     6841 aatgcggcat gggtgtccga aaatatgaac cgggacacag gctgataatt tcctgccggt
     6901 gctcttgtta tagcagaatc cgtcattacg gacttcaccg gattcctcca ggaaattcct
     6961 caggcttgca ggtgacatcc ctagtgtgtc gcgatccaca tccacgaaaa caggctttgc
     7021 acccgtgtag gcaatagcgt tggctgtagc aataaaggtg agcggctggg tgatcacctc
     7081 ctcatcgcgc tcaacgccgg caagcagcaa tgccatatgc agtgcttccg taccgtttac
     7141 gcatacaacg gctcttttgg caccggtata ggctgctgtt acctcctcga acgtatcaac
     7201 gaacttacct acacttgaaa caaaggtaga atcaatgcat tctgccagat atctcttctc
     7261 attgccgatg aagcggggct cgtgaagagg gatgaaagct tcgggttcgt taaaagcttg
     7321 ccttatgaaa ttgacgattt cttttaattt atccacaaat ttcaggaaag agggtcaata
     7381 ttgagaaaat gagaaaatgg ttttcaaacg tcagactgat tgagaatttc atctacctgt
     7441 tgcttcagaa caggaatatc ttcaactgca agtttccata ccattgaaag cgttatacca
     7501 aaatattcat gaaccagaat atctctcatt ccagcaatgg ctttccaggg gatgtgattc
     7561 cattttagcc ttttatcttc tggaatgtgt ttcgcggcct cgccaattat ttgaattctg
     7621 cgtaagacag catcctgttt ttcagggaaa ttgtaaaaag caccttcact aagatcagcg
     7681 atataggaag caattaaata agatgattca gaaatgtctt caagataaat gcgtatgtca
     7741 cgcttccttg tcataaagca atgataatct ctttagcaag catagatttt ttcatcctct
     7801 ccttaattgt tgatcgcaca attaaatcta ccttacggtt taatacctct tctaattcta
     7861 tttttatttt tgagaatgtt agtaaactta gagactgact gatttcaacc accacatcca
     7921 aatcgcttgt ttcagaataa tcaccatacg ccatagaacc cactatggat gccgaattta
     7981 ttccgtactt tttgaacacg ggtttgcata attttataat atgatcaata tcaacttgca
     8041 ttgaacaaaa ataatgtata ttatcgagtc acctataaat tataaatccc cggcttgtat
     8101 ttcgccaggt tttccggcct ggagaaccaa tcgcaggtta tttccagccc tttccggata
     8161 tcatgttcag gttgccagcc ggtgagttct tttataagcc tgttatcacc ccataacctg
     8221 aacacttctg aattttccgg ccgaatcctt gcttcatccg ttacaaactc cacacccgaa
     8281 ttcatgatct cccggatcag cccaaagacg tctttcatcg aaatctcata attggaactg
     8341 atgttaattt cttttcctaa agccttatca cattgggcca gagctatgaa gccagaacag
     8401 gtatctttca caaagttcag gtcgcgtgtt ggccttaaat cacccagatt gatctgtttc
     8461 atcccggatg cgatctggct gattatggtt ggaattatag cccgcgccga ttgccgggga
     8521 ccgtaagtgt tgaacggcct cgcaatggtg accggcaggc cgaaagcatt gtaaaagcta
     8581 agcgccatgg catccgcccc gatctttgtg gctgaataag gcgattgcgg ctgtttcggg
     8641 tgcttttcat caatcggcac atattgggcg gtaccataaa cttcagaggt agacgtgtga
     8701 attacacgga ttcctccgtg ttcaagcgcc gcctggcaga tgttgagcgt tcctttgata
     8761 ttggtatcca cgtagctgtc gggagccaca taggaataag gaatggcaat cagggccgca
     8821 aggttgaata taatatctgt gtctccggca atttttttta taaaatgagg atcacgaata
     8881 tccccggtaa tgatttccag gtttggataa gccggtatat cttcaagcca tccccagaag
     8941 ttaaaggaat tataaaatga aagcgccctg acgttgcatc ctttttcaag caatgcttcg
     9001 accaggtgcg aaccgataaa cccatcggcg ccggtgacca ggacagtctt gtttgtcagt
     9061 tccattcttc ggatttattt ggaaaaatga gtcttataaa tttcctgggc tttctcatag
     9121 tcatctacct gaccaatatc cagccagtat tcatagatct gatacttggc aacaggaatt
     9181 ttctcattca acatcttatc aatgagcaaa tccatgccaa aaaaggtatt atcaggtatt
     9241 aatcggaata tttcaggtct gaaaatataa atgcctgcca gcacattcat cctgatctgt
     9301 ggtttttctt ttatataagt aacgtagtcc ccttcaaatt caatgttccc aaactgaaaa
     9361 ggagtgatgt attcttttac cgaaatggta aggtctgcgc cggtcttcac ggcaaaatcg
     9421 tagaaatttt tgtaatccag caaggtaaga atgtcgccat tcatcatcag aaaagggtcg
     9481 gtaagttcat ttttcaacaa agtaattggc ccggctgttc ccagcggttt atcttccttg
     9541 ctgatcttaa gctttatccc gaaacgcgat ccgtcgccga aaaagttctc gatatattcc
     9601 gacttatagt tcgttgccag gaagatctcg ttaaacccgt gctttttcaa atgttcgatc
     9661 tggatctcca ggaccgattt ttcgccgatc ggcaggagcg gtttcgggat gatctgggta
     9721 aaaggccgta agcggctgcc gagtccgccg gcgaggatta ctgctttcat ttgttggaag
     9781 ggtaaatggt taaatggtta aattgttaaa ctgttgaatt gttccagggt tcctggtttc
     9841 cgggttccgg gttccaagtt ccgggttcca ggttcctaat ttcaaatttc aaatttccta
     9901 ctgcctactg cctactgcct accgccgact gacgactgac agtttttcat tcttccagca
     9961 accattgcca tgcgggtatg atagtgatat tatccggtgt atcaattgtg tcacctgcat
    10021 catacgcgat gagcacccct gtatccaggc catatgtccg catggcttcc tgaagtcctt
    10081 taatttcgcg acgcaggttt gtggcatcaa cggtccatgc cacctgtatg gcttcggtaa
    10141 cctttaagcc ttcttttacc aggaaatcgc attcgctttt tcctgcatga taataaattt
    10201 cttttccccg gcggagcaac tcaaggtaga cgatgttctc aagaatgcgc cctttgtttt
    10261 cagaaaaatt aaatcccagc ctgtttgata ctgccggatc tatggcatac actttcttgg
    10321 gattcatgat ctgttttttt acagaataat caaatttctt caggtaaaaa aacaggtaag
    10381 attgttcata ataccaaaga taatctttga tgctgctcag gctttttacc cccgaaatgt
    10441 tctgtaatgt agagtaggaa aacaatttcc ccgtatttga tgcaaaatac agcccgattt
    10501 gcttgatttc attgacctgg cttaacctgt aacgggatat gatgtcgcga tacaggatat
    10561 cctgaaaata agcgtttata agttccaggt cattttcttt caccactaac gggaaaccgc
    10621 ctatctcgaa gtagctatta aaatccttta ccagtaagga cttttgtttt ggggttatcc
    10681 ggcccgggat gtaaacattg ccggtatacc gaatatattc tgaaaatgaa aacgggaata
    10741 gctccagtat tttgttccgg ccggtcagac tggttgaaat ctgtgagctg agtaattttg
    10801 catttgaccc ggtcacgaaa attttagtgc ccttttcgta catgcggttc acaaattttt
    10861 cccagctgct tatattttgt atctcatcaa agaacaagac aggatcgttc ccatatgttt
    10921 caatgtgctg gttgtaaatt tgttccagta tcgaaacatc cgggatgatt cgttcgtcgt
    10981 caaaattaaa gtagcagtag ccttgctcat ccagcttcat tttttctttg attagataaa
    11041 gcaaggatga ttttccgcac cttcttatac cggaaattat aacaacctgt ctggttttta
    11101 tatacttttc taaatcaata tttcggtcaa taagatgttt ttgatgatta aaaacatctt
    11161 tctggtcaaa catgatttca ttaagtttat ctatattcat tatcataatt gtttgttcca
    11221 gaagaacaaa tataataata atacttcgtt agaaaagaaa gataatcaaa tccgggttcc
    11281 aaatttcaaa tttcaaattt tcgactgccg actgacgacc gacgactgac aactgacgac
    11341 cgcttcaaac tatattcccg ccccatataa attcattgaa gtgctttgcc ggaggaaacc
    11401 tgcccacaac gtaatgattc ctgagagtca accgggaaga ggttttctta agattttcaa
    11461 ggtcataata ggccggggtt tccttcactt cataggcttc cttatctgca ataaaatcta
    11521 tttcattacc ggttttaagt gaatagtatt tcaaatcgct atgttgcctc agttgaagga
    11581 acagtgcgtt ttcaaaagtg attccggaac ccggattagc aaaaacattc aagaggccgg
    11641 tatcgcaaaa ataaattttc ttcattttca caatttcacg gtcggtgttt tgggaatata
    11701 ccggaagcaa gtggatcagg taagttttct caaataattc tatataactc tgaaccgtag
    11761 gtcttgaaat accggtgagg cttgatatct tagaaatatc gagcttgtag gaacatctca
    11821 aagccagcat ttttatcaaa ttctgaacca tcgaaatgtt cctgaaatca gaaagtgatt
    11881 ttacatcaat attgatatag gaactgatga tatccgcaag ataatctgtt ttatccgttg
    11941 aatcgctggc caatacgacc tcgggaaatc ccccgtaaag aataaactca tcatagaatc
    12001 cttttaaccg gtgatactcg ctttcaataa acactttgct gattccgggc tgaccatgcg
    12061 gcatgttttt aaaagtgaga aattcaccga aatccagggg aaagagctca aagatctttt
    12121 tccttcccgc aagtgaatct gatatcaatc cttttaagta gaaagaactg gatccggtaa
    12181 ggatgaattt gatcgggtaa tgatcataaa gatattttat cacactggtg atttcaggta
    12241 ataattgtaa ttcatcaatg gcaatggtca ttttgctatt gatgtcaaga ccccgtccag
    12301 ccagagccag cagaatattt tcgtaatttt tttcaccaaa taacagacga ttatcaatcc
    12361 tctccagatc aatataaagc ttgtttttat cagcacagtc ttccaggagc tgtttaacaa
    12421 gagtagtctt tcctgttcgc cgcatgcctg taagtaaagt aatgggtttt ttttccaggt
    12481 gggctactaa accactatat atcttccggg gaataaacat tattcaaaaa tacaaaaaga
    12541 attactaaat gtaaagtata agttagcaaa tagtagatta ttttattaga tgaaggataa
    12601 attgttccgg ggttccagtg ttccaaattt ctaatttcaa atttctgacc gctgaccgcc
    12661 tgccgctcac agctcaccgt tcagcgatga aaacagcgga agggtatcta tgaattcttt
    12721 aaacatcagg atgcgcgtgc tgtgtctttt ttcaaaaacg agatgatcaa agtccctcgc
    12781 tttatgatcc aggtcaatct tttgaagcag atcgtgaaga gccttgtgaa gtgaggcggc
    12841 atcaccaata ccccatcttg ccgttaaaaa tgcccaatcg ggcccggatt gtccgagcag
    12901 aaacatggca tcataaaaat cgcgcccttt ttgcctggag atcagggccg acagcttcat
    12961 ggcagtcaga actccgtcgg aaggaacagg gaacgggaaa taaaaaccgc aaccctttat
    13021 atttaccttt tgtgtcgcat agggtacttc ctggtcctgg cattctattt taatcagaaa
    13081 tcgctcttcc cggtgtcccg ataatcccaa ttcaaacaat aattccggga agaagatgtt
    13141 ttttctgaat gcttgtaacg acttgtttat attctccttc gtttcaagtc tcaaaccctg
    13201 ccgctcaagg taaagtaaaa ctgaatcggt catttcatta aattcatctt ctgtgaaact
    13261 cttgcaatca aaatcaaggt cctcagaaaa ccggtctatt cctttgatca atctcaggtt
    13321 tgttcctcct ataaatacta ttttccttgc atatgaagtc gatgagagat aatcaaggat
    13381 catcaactgg atatattcct taaggatatg cttctgatga gcagggttat cacggacctg
    13441 aggagggaaa aagctttgga tctggtctaa ccggatcata aactataagc ttttaaaagc
    13501 attgaaactc gattttctaa cgatcggttt ctgtacctgg taacataatc ctttaggcgt
    13561 tccaaatcca ggctttctga tataatgtcc ccgtcaaggc gcaataattc catctcctgt
    13621 tgtgtattgt aatatggatt aagatataac aggtcaagta aagccttttc gggttgtgac
    13681 aggtgaactg tccagctatt tgagtaagtt ttcagatcat acccgaacat cagttccggc
    13741 ttgagagtct tataaaaata tgtgccactc gaattggtga aactggctgt tttaagcgat
    13801 gatacgcttg ttacctgcac aacagcttca gggatcaggc cataatagga aagcgctgaa
    13861 tgcaggctga tgtaagaagg acggtatatt aaattaccaa cataaaatga aaagcctggt
    13921 ttattaatat attcggggaa acaatagtaa ctgttgcgca atttgaccag gaagcctttt
    13981 ttaatccagc gccccaggtt gttcttatca aaacccggat accaggcaag tacctggttt
    14041 gtcgaaaagc aggctaagtc ataaaatgct attctgaatt cctgaaaact catgttattt
    14101 ctaaatatct gcaaatatac ggaataatag aaacaaaatt accgttgttc caatgttcca
    14161 gagttccaat gttccggggt tccagtgttc caaatttcaa atttcaaatt tcaaatttcc
    14221 tactgccttc cgccgaccgc ctactgccta ctgcctaccc ctcgctcctc gctcctccat
    14281 atcagcaaca gatcctttgc tgaaattttt cttcggtagt cttcaaactc ctccggccgg
    14341 aagattatgt accgtatctt ccgtttgatc agctcttcgg ttttgttggc aagctttacg
    14401 aggtactcct ggttaatgtc gtcgcctacc agcaccaggt cgatgatcgg ggcgtcgatg
    14461 ccgcgggcaa aatcacccgt cacataagcc ttttccacat tcccgagctt tgtcaccacc
    14521 cggtcgatga tctggtcgaa cccgatggtt ttaagcagga tgttgtggat atcggaaaac
    14581 agcggatgtc cggtgttggc gcggaaaacc tttttgttgc ctttgatctc cgacttcagc
    14641 aatccggctt cttcgaactt attcagttcc agccggatag catttgtgct ctcaccgaat
    14701 tccgattcca ggttgcgcaa atgggccctg gcaccggagt tcaggaagaa cttcaggagg
    14761 agcttgagtc gggttttgct ggttatgagg gtgtcgagca ttggggtgag cggtgaaggg
    14821 tgaggggtga agcgtgaggc gtgaggcgtg aggggtgaag ggtgaggcgt gaagggtgaa
    14881 gcgtgaaggg tgaggcgtga agcgtgaggg gtgaagcgtg aagcgagatt atttgttttt
    14941 taggtagcgg ataaggccta ttagcatcga tcttattttt tgaagagaat tatcgatttc
    15001 tttgctatct gcctcattta aataattttg atctgccgat ataatgaaaa gggtctctaa
    15061 ttcagcgatt gaacctaaac ttatataaag gaaatggata aattctttgt ctgattgtct
    15121 ggcagcgcct tcggatatat tcgccggaac agatactgca gctcttctga tttgtgttat
    15181 aaggccgaat ttttcaatat ccggaaatct tgaagtgatc tgataaacca tctttacaaa
    15241 atttatcccc tctttccaaa catccaaatc cttatgattc attacaacct cctttcacgc
    15301 ttcacgcttc acgcctcacg cctcacctga cacagcttcg ccccgtcagc tacatcctga
    15361 caagttttct cttcacgttt cacccttcac gcctcaccta aatcgagtag caaatttact
    15421 cattctttat acggttgtca agaggaaatt gttacttttt cgttaaaatg gttggattag
    15481 aacgcgaata acacgaatga aacgaattta tacgaatttt ttatccgtga tcatccgcca
    15541 tctggcggat tcgcgtcatt cgcgttctat cttattatcc ggagatcttt cacgtctata
    15601 ttcaaattca ggaactggcc gagcgcttcg atctcgatgc gtactttgcg ccggccctgg
    15661 aattcgatca gttgccccgt gagccccctg agcggaccgc ggatgatttc cacggcggcg
    15721 cctggtttca gatctttcag atcctgttcc tccggcaccg tttcggtttc ggagaggtaa
    15781 taacggatcg catcgatctg gttctgcggc acggcgacgg ctttcccctc aaaagtgata
    15841 tagcgcacga tccccgcggt gttcagaacg tcaaaatagc gggcaagggg gatgtggacg
    15901 aaaatatagg aacggaacag cggctcctcc acccatttct tccggtcgga ccactgtttc
    15961 agtttgcgct ccagtgggag ataggcttcg atcccttttt cgagaagatc ggcatgagct
    16021 ttcttttcag ccctgggatt ggtatagacg gcaaaccagt tcaaagttca tcaacgcaag
    16081 tgcttatagt accagtcgca tgccagttca aagcctttcc gtgcgtcgaa ctgcgggttg
    16141 taaccgagta attttcgggc tttttcgatc gaagcgtggg agtgcggaat gtcgccggcc
    16201 cgctcggagc cgtagacagg ctctatcttt gcgatctcag ggtcgtattt cgacaggttg
    16261 tcgcgcagga tgctgaaaag ctcattcagc gtggtgttgg cgccataggc aacgttgaag
    16321 actgccccct ccccaaagtc ccctccccct gcagggggag gggattcagg ggtaggggct
    16381 gtagccgctt tctcattcgc ttccaccaca ttatcgatat aggtgaaatc tctcgaatag
    16441 ctcccgtcgc cgttgattac ggggcgttcg tgcttcatga gctgcgctac ccatttgggg
    16501 atgacggcgg cataggcgcc aaacggatcc tggcgccgtc cgaacacgtt gaaataacgg
    16561 aggccgatcg tctccaggcc atagatatca taaaaaaccc gggcatactt ctcgttgacg
    16621 agcttggtaa cggcataggg cgacagcggt tcgccgatct gttcttccac ttttggcaag
    16681 gccgggctgg ttccgtagac cgacgaactc gaagcgaaga caaagcgttt gacttttgcg
    16741 tcgcgggcag cgatgagcat gttcagaaac ccgttgatgt tgatctcatt ggtccgggcg
    16801 gggttttcga tggatcgggg caccgagccg agagcggcct ggtgaaagac atattgcacc
    16861 ccttcgcagg cctgccggca tgtctccggg ttgcgaatat cgccttccag gaacgtaaac
    16921 cggggattgg aaaggaatgg ctcgatattc ctcttaaaac cggtgatcag gttatcgagg
    16981 cagacgactt cattgtcttg ctgtagaaaa gtctcgcaga gtgctgaccc gatgaaacct
    17041 gcgccacccg taaccaaaag cctgacattg tttaattcaa aattcaaaat tcaaaattta
    17101 aaatttatac tatcatgccc actcccaccg tattaaaagt tccttcctcg atcaggatga
    17161 tgctcccggt ggtccggttg cgccgatagc tgtcgaaata gagcggtttg gtggttcgca
    17221 gctgcaccct tccgatctcg ttcatccgca gctcttttcc atcgggcatc cgctcgagag
    17281 tgttgacgtc gaccttgtat ataatctctt tgatcatcgc cctgacctca gaggtggtat
    17341 gccggaggta gtatttcatt ccgggggcca tcggtttttc attcatccag cacatcatca
    17401 cttcgatatc ctgggagatc tcgggcacat tgttttccct gacgatcata tcgccgcggc
    17461 tgatatccag gtcatcggcc aggcggaaag ttaccgacat cggcgggtag gcttcattca
    17521 gttccgtctc gtacatatcg atcgattcga gggtagtgat gaaccctgac gggagcacca
    17581 tcaccttttc tcctttatga aaaacgcctc cggcgattcg ccctgcatat ccgcggtaat
    17641 cgtggtattt gtcggagatc ggccggatga catactgtac cgggaaacgg ggatcgatat
    17701 ggttctggtc gctggcaaca tggacatttt ccaggatata gcgcagggtg cccccttcgt
    17761 accagggcat gttgttcatt ttctccacga cattgtcgcc attcaaagcg ctgatcggga
    17821 catactggat atctttcacg tcgagtttgg cggcaaaagc gccgaagtcc tgtttgatct
    17881 tttcatagac ctcttcgctg taattcatca ggtccatttt gttgacacag atgatcaggt
    17941 gtgggatctg gaggagcgaa gcgaggtagg agtggcggta ggtttgctcg gtgacgccgt
    18001 tgcgggcatc gatgaggatg atggcggcat tggcggtaga ggcgccggtg accatgttgc
    18061 gggtgtactg tacatggccc ggcgtgtcgg cgatgatgaa tttgcgcttg ggggtggcaa
    18121 aatagcggta agccacgtcg atcgtgatcc cctgttcgcg ttcggcacgg aggccgtcgg
    18181 tgagcaaagc caggttgatc acttcgttcc ctttgcggat gctggcccgc ttgaccgctt
    18241 cgagctggtc ttcgaagatt gatttgctgt cgtagagcaa acgcccgatc agcgtgctct
    18301 tgccgtcgtc gacgctgccg gcggtggtga accgcagcag ctccatctcg aggtatgctt
    18361 tgcttgaaaa attctcttcc acaacaaaat agttcaaagt tcaaagggca aagttcaaag
    18421 tataattttc agcggatagt tctgttcatc atgccatgag atctttgccc gtttctcttt
    18481 gcactttgaa cttttcactt tgaactatca aaagtagcct tcttttttcc ggtcttccat
    18541 agccgattcg gagcggaggt cgtcggcgcg gctgcccctt tccgtaacgc ggatagccgc
    18601 aatctcagca ataatatctt caagggttga agcctcggag acggtcaggc cggtgcagga
    18661 gatatcgccg atggtgcggc aacggatcct catcctgacg accttttcat tctccttttt
    18721 cttcatgaac ggggcgtcgg ccagccagac cccgtcgcgg ttaaagacat ctctttcgtg
    18781 ggtaaagtag atattgggta tgtcaatgtt ttccaggtag atatactgcc agatatccat
    18841 ttccgtccag ttgctcagcg gaaaaacccg gaaatgttcg ccaacccgct ttttaccgtt
    18901 gaagatattc cacagttcgg gacgctggtt tttcgggtcc cattggccga aatcgtcgcg
    18961 atgggagaaa aatctttcct tggcccgggc tttttcctca tcccttcgcc ctcctcccat
    19021 ggccgcatca atcttgtacc tggtcaatgt atcgagcaac gttaccgtct gcagcaggtt
    19081 ccggctggca ttgtatcccg tctcttccac cgctcggccc gtatcgatcg attcctgcac
    19141 cgacccgacg atcagctctg cgccgagctt gctgacgagc tcatcgcgga aggtcatcgt
    19201 ctcctcaaaa ttatgtcccg tatcgatatg gatcagcgga aaggggagtt ttgccggcca
    19261 gaaagctttt ttggccaggt gcaccatcac gatcgaatct tttccgccgg agaaaagcag
    19321 ccccggcctt tcaaactgcg ccgccacctc cctcatcaca tagatcgatt ccgattccag
    19381 ttcacggaga tggttcaggt tgtatttgcg ttgatgatca gtcatttttt tagtcgttag
    19441 tcgttggtcg ttggtcatag actgccgact gctgactgcc gactgccggc tgaattttcg
    19501 gcagcacaaa agccagcaac ttctgcaccg attcttcaat gctcagcagg tcggtacgga
    19561 tttcgagatc ggcttcttcg ggcagttcga aaggggagct gatcccggta aaatctttga
    19621 tctcgccctt tctcgctttg gcgtaaaggc ctttgacgtc ccgtttttcg catacctgta
    19681 aaggggcatt gatgtagact tcgataaaat catctttgcc aatgatgctt cgggccaggt
    19741 gcctgatctc ctgcgtaggg ctgataaagc tgttgatggc gatgatgccg caattcagga
    19801 acagtttcga cacttcggcg atcctgcgga tattttcccg ccggtcatca tccgaaaagc
    19861 tgagattgtt gttgatgccg ctgcggatgt tatccccgtc aaggatctgg accatgtaat
    19921 tgcgtgcaaa caactcctcc tccagtctct tggccagtgt ggtttttccg gcgcctgaga
    19981 gccctgtaaa ccaaagcact actgctttct gcccgaggcg cttttcctta tccccgcgct
    20041 ggattatctt gtgaaaaaca ggaaaaatat tttccggcat ggtcttcata aaaaatgcaa
    20101 aggtcgtaaa ttaataccgt aaaatcaggg atcatgactt cttgaaaatc cggctcatgg
    20161 tcgattcctt ctccttctcc ttatcatcgg aataatatcc gtatccatac ccgtaaccat
    20221 acccataccc gtaaccatac ccataaccat atccgtagcc atatccgtag ccatatccgt
    20281 atccatagga tcctttggtg agttttacgt cgttgatcag aatgtggata ttggggatat
    20341 tccgtttttc gatatcgtta atgatcgagc cgaagacttt tttaagagtg taattctgac
    20401 gaacggcaaa gaggttggcg tcggtatatt tcatgaggag gaaggcatcg gtaacgagtc
    20461 cgacgggggg tgtgtcgatg ataatgtaat cgtaatcctt cttgagacgt tcgaacaact
    20521 cgtcggtttt cggggaggcg atcaattcgg aagggttcgg gggaaccgga ccggccatca
    20581 cgatgtcgag gttatcgatc atcgaatgct gaatgatccc ttcgtaagtg cttttgttta
    20641 tcaggtagga gctgacccct tcggcatttg aaaggccaaa atcctggtaa attttcggtt
    20701 tcctgaggtc aaatccgacc agtaccgttt tcttgccata aagggcaaaa atagaagcca
    20761 ggttgatgga tatgaaggtt ttgccggtgc tgaccatgtc ggaggtcacc agaaccgtga
    20821 tctgttcttt gcctttcgtg acaaaggcca ggttggtacg gatcgaccgg aacgactcgg
    20881 caatggacga cttgggcgat tcggccacga caatggtatt gagacggttg ctatggatga
    20941 catgcccgat gatcggcaaa tgggtaatct tttcgatatc gcctctttcc acgatcttat
    21001 cgttgagata atctttgccg aggacataaa gcaccggaag caccaggcca agaataatgg
    21061 cgatgatgta gttcaggctt tttttgggat aaaccggcga ggcgtcgagg agcctggccc
    21121 ggtcgacaat ttcattgtct gaaacatttg atgcccgggt tatctgcgcc tcggcacggc
    21181 gttgctgcaa aaagttgtaa atattgtcgc tcagtgtgaa ttttctcgta attcccagca
    21241 attccctttg tgttttcgga atattggtca tttggtctgt cagggcgttg atccggctgt
    21301 tgatatcttt cagggtcaga tcggaatttt tgacaatgtt ggtgatattc tccagcaggg
    21361 catttttcgt attttcaatc ctcagatcgt atgcaatgat ggtcggattt ttttctgtcg
    21421 atgtcaaaag ggcttctgca cgctcattga acaaggtgga cagttcctgg atgaggctgg
    21481 tagtgagagg atcttcgata cccattgccg aaggcacaat aaggccctgc tgtacgtcct
    21541 tattggtttc cacgtatttc ttcagcgatt tgtagtacat attcttgatg ctcagatcgg
    21601 ctttctgggt ttccagcgtt ttcatataat caaccgcctg ctgcgactgg aaatcgatat
    21661 ccatgacgat attctggctc cggtagtcct gcaattccat ttcggcataa ttcagcgaat
    21721 cgctgatgtc cataagctgt tcgtcgataa aacggatcgt gttgctggcg ataaggtttt
    21781 tcttttcgag gccctgggca agatattccc gggttaacat gttaaggaaa gccacggcct
    21841 ttgcttcatt gaaccccctg agtgatattt tgagaatgga agcgtctttg ttgatcggct
    21901 caatattcaa cccctggtac cgtccaacca gggatttcag atctttaaag acaaatgcca
    21961 gcttcttccc cgccaggttg gattctttaa cacggggatt gggtacaata tgaaaactgc
    22021 atgcttcatt ctccagctgt tcgccgaaat agagtgtatc attgatcttg aagaaaggat
    22081 attcctgatc cgattcttta aattgcgaat aactatagag ctttacattt tcgtattcgg
    22141 cctgtatggt atattttgag gaggaaagaa tggtcacttt gaattccacg ttaaccggct
    22201 gcagatggct ggtatcaaat acaacctcgt aagggctgtt tccgtaaagt tctttagaga
    22261 tgaaattttc ttcttcaaaa taggccacaa aaaaatcgag gttcctgatc gtcctttcgg
    22321 tcagggaata cgatgtgagg acgccgattt cgttttcaat attctggagg tcattgcgga
    22381 aacccagtcc gatcagttgc tggggatcga ttttattccc cctgtcggat ttaatgatga
    22441 ctgttgtact gacctcatat accggtttgg tgtatttgtt gaataagaag gcaatgatca
    22501 gcgccacgac aatggtcagg atgaaaaaat accagtggcg gtataatttg aaaaacaggg
    22561 ctttataatc aaaaccctct tcctgttgct ggatgggctg agtttgttga taaatacggg
    22621 tattgtcgtt ttccacggta ctgattattt ggtaatgacc tcgtagagca ggagcgatgt
    22681 tgtgataaca ctgaatatga tggcataagg aaattccgta aaggcgaacg gtttgccttt
    22741 gaccggctcg gcatagatca tgtcgtttgg catcaggtaa tagaactccg actcaaggat
    22801 actgttgtcg ttcaggttaa ggcgatgttt tttcaatccc ttttcggttt gcctgaccag
    22861 aaggatggta ttctttttgg caaaccccgt catatctccc gccatcgcca gtgcttcaaa
    22921 tatatttatc ttatcctggt aaatcacata tttccccgga ttattaaatt ccccgaaaag
    22981 agagattctg aaattggcca gtttgacaat caccactgta tttttaacat attcattcac
    23041 ttcgatttgc aaaactttct ggatctcttc aatggtaagg ttatcgacca ttactttgcc
    23101 gataaggggg aattcgatat aacccgaatc gttgacggta tagctgttta aatagattgc
    23161 ggattccgaa tagtaattat ttatcccgcc tgtatagtca aaataattac ttttttcatc
    23221 cagcgaattg attttgatat acagattatc gcccatatgg atccggtatt cattcggttc
    23281 gggtttctta aagtcgcttt tagcttcagt tttctgatgc tcatcctgga ggtacctgac
    23341 tcttttcatg ggaacacagg atgccagggc cagcaggaca atgagtggca ggaacactgc
    23401 tttcccggtt aaaaaagtag accaattcat aaaagatagt ttcacagacc tgtacattat
    23461 ggtgaaggtg cttttttagc acggattgca aaagtaatca taattcgttc atgagaacta
    23521 catcagtttc tggtaagttt gccaccagcg gtcgggcaat gcgttctggc atgccgtttc
    23581 gtaatcctgg taggagcagg ggaccaggtg gtgccgttcg taccgctcgc ggtgcttgct
    23641 gcggattggc acttccatcc accagcgttc actctttttg ctccggtaga acaccacctc
    23701 atccttgaaa tccttcatat ttacggtaaa cttcacaagc tcttttttct tgctgggggg
    23761 gaaatcgcct ttccggctat aaaatccgtc gataaaatac cagatcatct ggcctgccag
    23821 ctgggcggtc agcgattgcc ggtcgagccg cggattcagt tcatagatgc cgatggaagt
    23881 cagcttgtcg ctcaggccgg catagcgcat cacctggcag atctcctcgc cataaaagcc
    23941 gtttggcgtg gcatttgcgt ttcccggtgc gtctgatgcc cgcactgcgc ccatatccac
    24001 actgatcatg tcggcatttc ttaccatcgg ctctacctct tccaggttat tacggacaat
    24061 acccaggcgg taggtgtcga aataaagatt tttcatcagg gtgatcgcct gctggtcgac
    24121 aaaataggtt tgaaaaccga tattggtata attgaaaaga taattgggct ggtgaaggat
    24181 gatgtggctc atataactgc gggagtcgag cttctcctgc cccgtcccga tgtcgaactg
    24241 tgagtccacg gaagcgatgt tgatgatcct gccgatcttt tcatacgctt tgtactgggc
    24301 ataggtaatg tcctggctcc cgcccaggat gaccggaacg atgttgtgct ccagcagctc
    24361 ggccatgatg gccgttacgg caaaatacgt atcttcaatc gaatgaccct gcctgacgtt
    24421 tcccaggtcg accatccgca gcttgaaatt cccctggtac aatttgtaga actccttccg
    24481 gatggcatcg ggacccagtc cgcagccatt attgaaaaag cagttcctct cttcacctac
    24541 gccgataaag gccaggtcca cgtgatcgag agaggggaaa tcgttgggtt cgatataggc
    24601 ctttaccacg tcgcccatga gtttgcggcg tgtcttttca ctgaaatgat aacctttcag
    24661 ttgaacgggc tcgaaataaa tagagaggtc catgtagcgt ttttatccgt ggaaagtata
    24721 cggatttgac acggaaatgt tgccggtttt atgatttttt ccttgtcggg atggttttct
    24781 ttttcccgct tttttcctgt ttttcggcaa tctggaggca ctcatccagg ctcagcgagg
    24841 caggatcagt ccccttgggg attttataat taaccttgga gatcgctatg tatggcccga
    24901 aacgaccatt catcaccacc acattctcgt tttccgggaa tgatttaatg atcttttccc
    24961 tgtcctgctt ccgtttcagc tcgatgagcg atatggccct ttcctggtcg atcagcgccg
    25021 gatcgtcctg ctttgtcagt gaataataca ggttattatg tttgatataa gggccgaatc
    25081 tgccaacggc gacgatcatt tccgcgtttt cataaagccc caggttgcgg gggaatttga
    25141 aaagctccaa tgcctgttcc agcgtcagcg tgtcgaggga ttgccccttg ccaagacctg
    25201 caaaacgcgg tttttcgtca ctttccgttt caccgatctg gacgacaggg ccgaaccggc
    25261 ccagtttcac gtaaacattc tttcccgacc cgggatcgat gcccagcaac cgttcgccgc
    25321 tgaactttcc ggtcgattcg accgtatcgc ggatctggct gtggaagggc ttgtaaaact
    25381 tccggatcat ttcgttccat tcctttttcc cttccgcgat ctcgtcgaag tctttttcaa
    25441 ccgttgccgt gaactggtaa tcgatgatat cctggaaata ctgcatgagg aaccggttta
    25501 ccagtgtgcc gaggtcggtc ggtaacaact tgcctttttc attgccggcc ttatctttct
    25561 tttcatgcag ctttatctca ttattcttta aggtaagaag gtcgtaggta cgaataaatc
    25621 cttccacatc gcctttttca acgtactctc tcttctggat ggtggaaatg gtcggcgcat
    25681 atgtcgacgg gcggccgatt cccagttctt ccagccgttt taccaggctg gcctcggtaa
    25741 acctccgctg gggctgcgta aaccgctgtt cggcattcat ttccagcatt ccgggctgat
    25801 cgccgacaga aaggggtggc aggatactgc gcccttcttc atccgaatca tcatcaaacg
    25861 actcccggta aactttcaga aaaccgtcga atttgatcac ttctccctgg gcaataaatt
    25921 cctccccgcc cagtgttgaa gcactgatgt taacaatggt cttttcggtc agtgcatcgc
    25981 tcatctggct ggcaatggtc cttttccaga taagttcata aagctttttc tgtgttgcgc
    26041 tccctgaaat ggctgcgttg gcaatgtatg tgggccggat agcctcgtgt gcctcctgtg
    26101 cccctttggt tttcgtggca aattgccgtg ttttcgaata ttcggggcca tacagcttga
    26161 tgatctcctc cttggccatt gccagcgcca tcttcgacag cgttaccgaa tcggtacgca
    26221 tataggtgat caaaccttct tcgtagagtt gctgggcaac tcgcatggtg ttggccaccg
    26281 aaaaacccag tttgcggctg gcttcctgct gtaacgtcga tgtggtaaat ggcggagcgg
    26341 gcgatttttt cgaggggcgt gtttcgatcc cggtgacttt ataggaggcc ccgatgcatt
    26401 tttccaggaa tttccgtgcc tcttcccgtg tcttgaaacg ggttgggaat tcggcttcga
    26461 atttgtattc ggcccctttg atcgttacgg taaattttgc cgttacctta aaagaggagg
    26521 tctctttgaa agccttgatc tcatcttccc gttccacgat cagacggacg gccaccgatt
    26581 gcacacgccc tgccgacagt gccggcttaa cttttttcca aagcaagggc gaaagctcga
    26641 aaccgaccag cctgtcgagc acacggcgag cctgttgcgc gttcaccagg tcgcggtcga
    26701 tctgccgcgg gttgtcaatg gcatcgagaa tggccgactt ggtgatctcg tgaaagacga
    26761 tccggcgcgt ggctttttca tccaaaccca gcgccgatac caggtgccag gatatggctt
    26821 ccccctcgcg gtcttcatca gtggccagcc agaccacttc cgcttctttc gccgcttttt
    26881 tcaactcacc cactaccttt ttcttctccg gggagatctc gtagatcggg atgaaatctt
    26941 tctcaatatc cacacccagg cccttttttg aaaggtccat cacatggccg aaactcgagc
    27001 ggacggtaaa atccttcccg agaaagcgct cgattgtctt tgcttttgcc ggcgactcaa
    27061 caataaccag gtttttgatc atgctctctc tttaattatg atgcaagcta caagctgcaa
    27121 tctacgggcg ttttcacgcg attcctcctt caaaaaatgc cagtgcagac tttgcggccg
    27181 caaaagtaca acttttctta ttaatcggat tttcaggtaa aatatacccg gtcgaccgaa
    27241 aataattttt aattattaat ttataaaaca gaaaatcagg aagttagacg ttggataatg
    27301 cgcgctccaa cgtgtcgaat ttgtggtgat agtcttccat cgagcggcgg atgatctcgt
    27361 gcgcctcatc aatcccgaat acatgcgtga tgacaacatt gctctccttg ccgggaagat
    27421 ccttgtaggt caggaaatag tgtttgagcc ggtcgacgac caggacgggc agttcggcga
    27481 tatcgttgat gtgtccgtaa accgtatcgt tgttgagaac cgcgatgatc ttgtcgtccg
    27541 actcatcccg gtcaatcatc cggaaccctc cgaccggacg ggctttgacg agaatgtttc
    27601 catgtgagat ctccttttcc gtcaacacgc agatatcgat cgggtcgcta tcgcctttga
    27661 taccggtacg tcccgatttc tgcatacaga actcggccac cgcggtggag ctgaatgtct
    27721 gcggaacaaa tccgtataat gccggcacaa cgttggaata cttctgcggc cggtcgatgc
    27781 ggaggtaccc cgactccttg tcgatttcgt acttgaccgt atcggtcggg accatttcaa
    27841 taaaagccgt gacgatgacc ggtgcctcgc ggccaatatc cacgccatgc cagggatgtg
    27901 atttgtatct cagccccatc agccggccta tcggatccat cagaatgttt gacataccta
    27961 ttttttaatt ttcaaatttc caaattttca aatttccaca ttgttttcat ccaactgtta
    28021 tgccttcctt ccagggtttt ccgccagaaa cgctttccag ccggtaaact tcttctcttt
    28081 tttgcccccg cctggattat tctgaaaata gtggcaaacg gccgcggcaa gtccgtcgga
    28141 agcatcgaaa tactccggcg attcgctgaa tttcagcaga tgctgcagca tcagcgccac
    28201 ctgttctttc gaagcgctcc cttttcccgt gatggcctgt ttgatttttc tcgggctgta
    28261 ttcaaagatc gggatggtcc gcgacagcgc cgccgccatc gctaccccct gagcccggcc
    28321 cagtttcagc atcgactgca cattctttcc aaagaacggg gcctctatcg ccagctcgtc
    28381 gggatgatat tcttcgatca gggcgatggt gcgctcgaaa attcttttta acttgtcctc
    28441 gtgttcggaa taccggtgaa gcttaagcac gcccatggtc agcaactcga tctgcttatc
    28501 cctgaccagc acgacgccat accccatgat gaccgtgccg ggatcgatgc ctaaaattac
    28561 cctttccgac gacatactgc aaagtgacaa atgacagaac aatcggcaaa aatacgcaaa
    28621 tacgtcaact tcctgttaag ggctgtcatt atcattttaa cttatggatt catttatcat
    28681 cagatattcc ttgaaaacaa gctcgacgac ctgtcggatg tcttcctgcg gcttgccgaa
    28741 cggcaggatg tgaaatggat cctggcttta atgatgcttt taatgtttat caactgggga
    28801 atcgaatctt ttaaatggaa actgctgatc tccaggattg aacacgtttc attcttccgg
    28861 agcttcaaag cggttatggc agggatatcg gtaagccttt tcatgcccaa cagaaccggc
    28921 gactatctcg gacgggtttt catcctcgaa aaagggaacc acgtggaggg cattttcagt
    28981 accattatcg gcagttttgc acagatgatc attacgttcg gcatcgggct cctgtgtctg
    29041 ctgacattca ccgatcatta cttcagggaa tcgctgaaca tcggcgaata catgttcatg
    29101 agcctggttt tcctggtgcc cgtcatggtg tttgccctga tcatgatcta tttaaaggta
    29161 ggtgtgctgg cggaatttct aagccgtttc ctgccgggca gatggccccg gctgaaagaa
    29221 aatgctgcca tattttccag gtacagcgcc ggcgagctca gccgggtgct gctattgagt
    29281 tttttgagat acatcgtttt ctccacgcag ttttatatcc tgatcagggt atttggcgca
    29341 ccggtacccg cggcagaagg atttgtcctc attcccgtga tctacctggc gatggcgctg
    29401 gttcccacct ttgccctcgt cgagctgggg gtacggggaa cggtttccct ttttattttc
    29461 gggctctatt ttgaaaagtc cggcatagtt gacccacaaa tcctgctggc gcccctggcc
    29521 gcttcttcat tgctctggct gatcaacctg gttctccctg ctgtaatagg gacattcttc
    29581 gtgttcagcc tgaaattctt ccggaaataa ttgatagtat ggcaaccggc ttcctcatga
    29641 tcctcatcct ggcattgctg atcgcattaa gctatctcgt tctcatcgcg atatttactt
    29701 tcggatggta caggaaaaat ggggaaaaga acttttctgg cttttcttta atgaagatca
    29761 gcgtcgttgt tgcggtaagg aacgaggaga gcaatatggc aggattgctg aacgacctga
    29821 tccggcagga ttatcccggc ggcctccttg aaatcatcat tgtggatgac cattctacgg
    29881 atcgcactgc cggagtcacc aggaatttta tacaggatca tcccttgtca gctattattt
    29941 ttattttgaa taatgatgaa aaaaaaaggg ggaagaaagc atctgttgag ctcgggataa
    30001 gccgtgccac gggtgaggtc attgttacga ccgatgccga ctgccggatg cgggaagggt
    30061 ggatcagggc gctggccggc cggtttacta aggagcacgt gcaaatggta ttcggccccg
    30121 tgacgatcgg taaacaggac aattttgccg gcctgttcca ggctatggag ttcgacggcc
    30181 ttgtcgcttc aggaggcgga gcagccctgg cgggttatcc tttcctttgc aacggggcaa
    30241 acctggctta ccgcagagag gctttccaca cgaccggcgg attcagtgac aacatgcgtt
    30301 atatatccgg cgacgacgtt tttcttcttc ataaaataaa aaaacaattt ggcgccggag
    30361 ccatagtatt ttgtaaagat gaacaggcaa tcgtggatac ggcgccggtt tccggtgcgg
    30421 gagctttcct gcgccagcgt atccggtggg cttccaaaag caaaggctac cgcgactccc
    30481 tatccattat tactgcaata accatcttct ctttcagtct taccgtctta ctgtccttcc
    30541 tggccggttt cacagatcca atgtatttca gtatttcagg cggattattc ctgttgaaga
    30601 tcttcgctga tcttcctttg ctggcgggga tcacgaaatt caccgggaaa ccgtttcctt
    30661 tcatgtggta cccggttttc cagctcattt atcccctgta cataattata gcagggatct
    30721 taagtctttc gaatagaaat aaatggtaat acgcaggtcc cgccaaggct ttaaatgatc
    30781 agcatggcgt cgccgtaagt gaagaagcgg tatttctggt cgatggcttc ccggtatgcc
    30841 tccatcagga aatcgtagcc ggagaaagcg gccgccatca tcatcatcga cgacatcggc
    30901 aggtggaagt ttgtcaccag ggcgtcggca acggtgaaat cgtaaggcgg aaagatgaac
    30961 ttgttggtcc atccccggta gggctttact tttccggaga tcgaaacagc cgtttcaagc
    31021 gccttcatcg tagtggtacc aacagaaacg atcttcttgc ggttctcttt tgcagagttg
    31081 atgatgccgg ctgcggtctc ttcgatgagc atctcttccg aatccatctt atgctttgtc
    31141 aggtcttcca cttcgatcgt gcggaaatta ccgaggccga catggagtgt gatttcagca
    31201 tagttgacac cgagcagctc caggcgtttc atcagctcac ggctgaagtg catcccggcc
    31261 gtgggagccg caacagcgcc ttcatgcttc gcatagatcg tctgataacg gatatcgtcg
    31321 agcggctcga cggtacgttt atggattttc ggaagagggg tctgcccgag gtcggtgatg
    31381 gttttcttaa aatcttcata cgggccgtcg aacaaaaacc ggagggtccg tccacgggag
    31441 gtcgtattgt cgatcacttc ggcaaccagc gagtcgtcat ccccgaaata aagcttattt
    31501 ccgatcctga tcttccgggc gggatcaacg agcacgtccc acaaccgcga ttcgcggttc
    31561 agttccctga gcaggaacac ttcgatcttt gccccggttt tctctttcgt accgtagagc
    31621 cgggcgggga agactttggt attattgatg acaaagacat ccccgtcact gaaataaccc
    31681 agaatatctt taaaggattt atgttcaatg gtgcgggtct tacggttcag caccatcatc
    31741 ctcgaatcat ccctgttttc aggtggtctg tctgcaataa gttcagaagg taaatagaat
    31801 ttaaactgcg agagtttcat aacgacacat tacatctttt caaaaagctg tgcaaaggta
    31861 atacaataaa gagggtttgt caactgatta ttaaggaagt tttaagtttt gttgattttt
    31921 gcgatcccat cattattgtt gcgcaaccaa aacctcgaac tggggcaggt ttatcgcatc
    31981 atcgacacga taatctccga tacgggtgcg ccggagagaa gccaggtatg cccccgagcc
    32041 cagcgcttcg ccgaaatccc tggccagcgc ccggatatag gtccctttgc tgcccgacac
    32101 acgaaaaccg acctccggca atgctatatg cgtaatctca aattcgctga tggtgatggt
    32161 taccgggtcc agccgcactt ccctggattt ccgggccgac aaataagccc gctttccatc
    32221 gaccagcttg gctgaaaact gcgggggaac ctgctgaatc gtcccggtaa accgctttgc
    32281 cgtatcccgg atcatctctt ccgtgatgtg atcgaccgga aatgtccggt caaccggctt
    32341 ttcggtatcg taacacggcg tcgtggcccc gaggatgaaa gtcccgcagt attccttatc
    32401 caggccggtg aggttgacaa tctgtttggt catcgcaccg gtacagatca gcaacagccc
    32461 tgtggccttg ggatcaagcg ttccggcatg accgaccttc agcttaacac ccgtcttttt
    32521 ttcgatcagt cgcctgatct tgttcacggc atcaaacgag gtccagtcga gcggtttgtc
    32581 gatgagcaac agctctcctt tgtgaaaatc aaacatcatc cgaaaataat ggcgatcagc
    32641 ccaatgatga agcaataagc cgaaaagtaa accaggttgc cttttttcac aattccgatc
    32701 atccatttgc aggcaaaaaa tccggagatg aaggctgcag caaaaccgat gaggagcgaa
    32761 gtggcgctga ccgtcgtttc tgtcatcatt tccgaaccgg ccaggtcttt tgcattggcg
    32821 ccgatgatag ggatcagcac catcaggaac gagaatttgg taaccagctc ccgtttattg
    32881 cccagaagca ggccggtgga aatcgtggcc cccgacctcg agataccggg gatgactgca
    32941 atggcctgtg cgatgccgat aatcagggaa tcggcaaaag tgattttccg gttattgttt
    33001 ttaataagat aggtcgaagc cagcaggagc gcggtgacga tcagcatgaa gccgacaaag
    33061 acaacccttc cggtgaacag cgattcaatt tcttccatga agaaaagccc caggatcacc
    33121 acggggatca tggagaggat gagcttgaaa ttgtattgca tggtttcggt aaaacgaagc
    33181 gctaccgcat ctttccacct gatggcaaag acttcacgga tcagctgcca gaggtctttc
    33241 ctgaacacga cgatggtact gagcacggtg gcaccatgga ccaccacggt aaaaacaagg
    33301 cttctttcgg gatcaacgcc caggatggct tttccgagct caaggtggcc gctgctgctg
    33361 accggtaaaa attcggtcag gccttgtaca aggccgagga tcagcgcttc aaaccaattc
    33421 atctttaaat aaaaggtcag gaattaatct ttgggttttt tcattatgga aaagataccg
    33481 acgacatatc cggcaaggat caggatcggc gcgagtgtca accgctgaaa attgaaaatg
    33541 ccgtagctga atgtgttggg atctttcgat ccgccgccga tcatcagcag gaatcctaat
    33601 acgatcagtc ccaatgcaat gaacaggagg cggtagtttt ccctgccaaa tgcaaatccg
    33661 gcgcgtgatt ccggctccgg agcctttaac ggcgaagctt tttcttttgc aaccggttta
    33721 gtctttgtca ccatctttta agaatttaaa acaaaagtat aaaaattcag cattaactat
    33781 acagttcatc ttccgtcatc ctgagatatt tcctgacggc aaaccaggtg gagacccatg
    33841 ccaggaacat accggtgagc atcaccagca aaaacacggc caggtaaagg tagagatcga
    33901 aaagatttac cagttcgggg acctgttgca tgaggaaata caggatcgtt accaggagga
    33961 tgatggcgaa gattgcgctg taaaagccct gtaaaactcc cctgacaatg aacggacgac
    34021 tgatgaaccg ccgggtggca cccaccagtt tcatcgtccg gataatgaac cttttagaat
    34081 aaacggataa acgtatagtg ttgtttatca aagcaatagc gatcaacatc aacaaaccgg
    34141 tgaacccgag caagacgata ctgatccggt tgacattttt attgatgaga tggaccagcg
    34201 atttctggta aaaaacctcc ttgacgacca cctcctgcat caggtttcgt tcgatggttt
    34261 caaggctttc cacattggca taggcggctc ttaccctcag gtcgatggaa ggcggcagcg
    34321 gattgtatcc caggaaatcg atgaagtctt cgcccagatc ggcggtcagt tcccgggcag
    34381 cctcctccgg ggagatgtac cgtgaggatt tgacgaaagg ttccgcatcc agcttttttt
    34441 gcaggatgat aatatcctcc gtcgcagcat cctccttcag gtacaccctg aacccgatat
    34501 tctctttcac atagtccgat aacttgcggg catgaagtat cgtcagtccc agcaaaccca
    34561 gcatcgtcag caccagcgtg atgctgacga tcgtcgtggc tacgctcgac tggtagcgcc
    34621 tccggtggta tctttcctcc attttcgaca tggcgacgaa gatagcactt tttcacggac
    34681 tctgcatcgg gtgattattt gtaaatttgc tgcctggata gcctctttgg caaagatttg
    34741 aaaggactta accacaaagg cgcacaaagt acttcacaaa gggccacaaa gttacagtta
    34801 caaattcaaa acctttgtgt gacttcgtgt aactctttgt gttcctttgt ggttaaataa
    34861 taaactttta ggctggaaca ctggaacaat tatggagtac aactttaacg cgatcgaaaa
    34921 aaaatggcag gattactggc gggagaataa aaccttcaaa gcctttaatc aatctggcaa
    34981 accgaaatat tatgtgctcg acatgttccc ttacccctca ggggctggac tgcatgtcgg
    35041 ccatccgctg ggttacattg catccgatat ttacagccgc tacaaacgcc tgcagggatt
    35101 taacgtgctc cacccgatgg gcttcgacgc ttacggcctg ccggcagaac agtatgccat
    35161 acagacgggt acccacccgg ccattacgac cgaaaagaat attgcccgct accgtgagca
    35221 actcgaccag atcggctttt cgtacgactg ggaccgggaa gtggtgacga gcgacccgtt
    35281 ttattacaaa tggacgcagt ggactttcat ccggcttttc aagtcgtggt acaacaggga
    35341 aagtaacaaa gcagagcaaa ttgataaact cgttaatatt tttgaacaag aaggaaacgc
    35401 gaacgtgaag gcggtttgca atccgacaga tacattcacg gccgaatcat ggaaagccat
    35461 gaccgaaaag gaacagcagc agatactgat gaactaccgc ctggcttatc tttccgacac
    35521 ctgggtaaac tggtgcccgg ccctgggaac ggtgctggcc aacgacgagg tgaaagacgg
    35581 tttttcggaa cgcggcggcc acccggtgga acgcaaacag atgaaacaat ggtcgctgcg
    35641 cataacggct tacgcacaac gcctgctcga cgggctggaa aagctcgaat ggagcgactc
    35701 cctgaaagag gtgcagcgca actggatcgg gcgttcggaa ggcgctgcag tgtatttcca
    35761 gttggcagcc ggcagttggc agccggcagc cggcagttgg cagtcaaata tgagacccgg
    35821 aacccggaac ccggaacccg gaacatttat agaggtcttc accacccgtc ccgataccct
    35881 cttcggtgcg acattcatgg tgctggcacc ggagcacgaa ctggtgccgc agatcaccac
    35941 gcccgacagg aaagagaagg tggccgaata tgtcgaatgg gcgaaaaacc gctcggagcg
    36001 cgaccggatg accgaagtga aacggatcac cggcgaattt acgggagctt acggcatcaa
    36061 cccgctgaca ggggaacaga taccgatctg ggtggccgat tacgtgctgg ccggttacgg
    36121 aacgggagcc atcatggccg ttccggcgca cgacagccgc gacttcgcat tcgcccgtca
    36181 ctttgacctg ccgatcatcc aggtggttaa aaaggaagat gaggaggaga gcgatccgat
    36241 gctctggtca gaatcgtatg acgccaaaga cggggtgatg gtcaattcgg gcttcatcac
    36301 cggactgaaa gtaaaagcgg ccattaccgc cacaattcgc aaaatagaag agcagggcgt
    36361 tggatacgga acggtgaatt tccgcctccg cgatgcgatt ttcagtcgcc agcgctattg
    36421 gggtgagcct ttcccgattt actaccgcga agggatgcct tatccgctcg acgaaagccg
    36481 gcttccgctt gaattgccgg aagtcgacaa atacctgccc accgaaacgg gcgaaccgcc
    36541 cctggcacgg gccaaaaact ggcacacacc ggaaggttat ccgctggaaa ccagtactat
    36601 gccaggattt gccggctcca gcggctacta tctccgttac atggatccct ggaacaacga
    36661 tgcctatttt tctaaggaat ccaacaggta ctggcaggat atcgatctgt acgtcggtgg
    36721 cgatgagcac gccgccggcc acctgatcta ttcccgcttc tggaacatgt tcctcttcga
    36781 tataggtttg gtatgcaaag aagagccatt caaacggctg atcaaccagg ggaagatcca
    36841 ggggcggtca agctttgtct accgaatcaa cgggaccagt aagttcgttt cttatggcct
    36901 taaagatcag tacgatacga cccgccttca tgtcgacatt gaaatggtgg ataacgatgt
    36961 cctcgatatc gaagctttcc gaaattggag agcggagtac cgtcatgccg aatttatcct
    37021 ggaaaacggg aaatatatct gcggatggga gatcgaaaaa atgtcgaaat caaagcataa
    37081 cgtgcagaac cccgatgatc tcattgtccg ttacggcgcc gatacgctcc ggatgtacga
    37141 gatgttcctg ggcccggtgg aactgtcgaa accctgggat accaagggga tcgaaggagt
    37201 tttccggttc ctgaagaaat actggcggtt gttctttaca gagcagggtg aatttcaggt
    37261 taccgatgag actccaactg ctgccgagct caaaatcctt cataaaacca tccttaaaac
    37321 caaagaggat attgaacggt tttccttcaa tacgaccgtc tccagcttta tgatctgcgt
    37381 gaacgaactc accgatctga aatgcaataa acggtcgatc ctggagccgc tgaatatact
    37441 catctcatct tttacccccc acatcgccga agagatctgg tcgctgctgg gtcatgacgg
    37501 aacgattgct tatgcatctt tccctgaatt tgacgaagaa ttcatccggg aaaatacgtt
    37561 taattatccc gtttcgttta atggtaagct gcggttccag ctcgagctgc cggtcgactt
    37621 cgcaccggcc gatgttgaag ccgctgtgat ggctgcaccg gaagcccgga agtggctggc
    37681 cggcggagcg ccgaaaaagg tgatcttcgt gccgaagaaa ataataaatg tagtggtatg
    37741 agttccaggt tccagattat aatacgacct gtcgggaagg tatttcacaa atctaatcag
    37801 cctgttttat tagtagtgag ggtgtttcaa aagtctgtta tttcaaatga tggcctgaca
    37861 ttatttcttg ctccaacgac taaagccgtt ggttcctatg gatttatgaa ggtgggaacc
    37921 gacggcttca gtcgtcggac taccaagaaa agtttgggat tacgattatc tcttggattt
    37981 ttatttcttt ttatttcaaa ccagcttatt agccaggata catttcgaaa agttgaaaac
    38041 aacacctttc agcacggcga attcctgaaa taccgcgtct tttacgattc ctgggtgacc
    38101 tactggatga ccgccgggta cggaacgatg gaaatttctc ccgaaccggt gaaaataaac
    38161 ggcagggaca cttaccatat cactgtcaat ggcaattctg ccggcctttt caacgtcttt
    38221 tacaaggtca gggataaatt tgagacttat atggataaag aggggctgat gccgctgaaa
    38281 tttatccgct atacccggga gggaggctat aaaaaagacg atacgatctt ttttgaccac
    38341 caggcccgga aggcggtgag tatccgcaaa acaaaagata ttacacagta tgtccaggat
    38401 atagtatctg ctttttatta tgtcagatca tgggatttcg atacggccga ggtgaatgat
    38461 acatattacg tcgatttttt cctggatgat tccctttatc acagcgaaat tgttttcatg
    38521 ggccgtgaat cggtcagaac cgatttcggg actttgccct gcatgcggtt caaaccacgc
    38581 gtcgctgtgg gtgagttatt ccaggatcca tacccgatgg agctatgggt gaccgacgac
    38641 cggaataaaa tacccgtttt gatgaagtcg gctgttttta tcggatcggt gaagattgaa
    38701 ctggtggaat acagagggtt gcgatggccg tttgaaagtc attagctcgc tgcgctcgcg
    38761 tcataaagtg gtcataaagt agtcaatagc tcgctgcgct cgcgttatta ggttgtattg
    38821 tgctgtacgg atgttataaa aaactttggc ataaatttag cttatccttc atgtaacttc
    38881 atgtcttctt catgtaactt cgtgaaatga tatttatatt cgaaaaaaca taaagaagaa
    38941 ccaaccgaat aatcaattta gccatgaata aatcaattct gttatttctc atcattgcca
    39001 taagttgtac taccatgggg caaaagtctt atgaaaaaga gtggaaggag gtggaagccg
    39061 ctgtccgcaa aggcctgccg caatcggccc tcaaaattgt ggattcggtt tacctggatg
    39121 ccagatcgtc ggataatgcc ccgcaattcc tcaaagcaac cttataccag atcaaacttc
    39181 gatccgattt ccaggaaaat tttatggaaa gcagcatcct gcaggttgaa actgagctga
    39241 agaccgcttc tgttcctgtt aaacagatac ttcactcgat cagggccgag ctctactggc
    39301 gctattacca gggcaaccgc taccgcttca tggaacgcac aggggtttcc aaccctgatc
    39361 tgaccgatat caaaacttgg gacctcaaaa ccctcgtcaa tacggttaca gaaagctacc
    39421 tggcatccgt cggccaggaa tatgcaaccc tgaagatcaa acttgatttt atcgatccga
    39481 tcctcgaaac ggaaccgggt tcaaaaatct accgtccgat gctgtatgat ttcctcgccc
    39541 accgcgcgct ggagtatttt gccaatgaag agcccgccgt ggtcagaccg gccgaaggtt
    39601 tcctcctcga ccaggaaaat tatttctccc ccgccggtga gttctcatcc ctgaaaatca
    39661 cgtcgcccga cgagaacgac ctgaaatggc gcgccctcct gatctatcag aatcttctgc
    39721 ggtttcattc gggcgatgat gatccctccg ccctcatcga tgccgacctg gccaggttga
    39781 aattcgtgca gcagcattct gttcttcagg ataaagacct cttatataaa aatgccctgg
    39841 ccgaattgca ggaaaagcac aaatccgctc ccggcgctgc tgatgtggcc tacgagctgg
    39901 ctgcatcgta taatctctcg gggacgttct atgtcaggaa ccagggcgaa gtaaaccgct
    39961 gggatctgaa gaaggcccgg gagatctgcg aaaaaaccat cagggattat ccgggaaccg
    40021 acggggcaaa gaattgcgct atactccttg cagagatcag cgaaccttca ttgaactttt
    40081 cggtgaacta cgccaatgtg cccgatcagc cattcctcgg ctccctgaac ttcaggaatg
    40141 tcagtacggt ctatttccgg attgtgaaag ctgatccgga agaagacagg aaccgccggc
    40201 aacgggaaat gggcgagaac cttgtcgcga aatacctggc cttgcaaccc gtcaaagcct
    40261 ggtcggtgaa actgcccgat gatggcgact tccagacaca taacacccag gtcagaatac
    40321 cttcgttgcc aaaaggttac tacgtgatcc tggcttccga taatgaagga ttttcttatg
    40381 aaaaccggcc cgtcgcctac tccccggcat gggtaaccgg catcagttat gtcagccagc
    40441 gcaacccgga agacgggaaa ctggagatct atgtcctcga ccgcgacaaa ggaatcccga
    40501 tgaaaggtgt ttccgtggcg gcttacatca gggaatacga ttataagaca cgctcatacc
    40561 ttgataagct gatcaacacg ctcacttccg atgaaaacgg ttatatttcc atggccgctg
    40621 tcaggaacga ttcaaagtca ttttttctga aattttccca ggggaacgac ctctatttta
    40681 cggaaaacta tttctacctc tatcccaccg gaccgacaga ggatacccgc atcactacct
    40741 actttttcac cgaccggtcc atctacaggc ccggacaaac cgtttatttc aaagggatcg
    40801 tgctggaacg gaccgggacg aaaagcgaaa tcaagcccgg ttactcccat gtggtcactt
    40861 tttatgatgt caacaaccag aaggtatcgg aaaaagcggt tacgacaagc gatttcggtt
    40921 cgttcaacgg catttttacc gcccctgccg gagggctgac cggtgaaatg agcatacggt
    40981 gcgagaccgg cagcgtggga ttttcggtag aagaatacaa aaggccccgc tttaaggtgg
    41041 aggtcgatcc gctggaaggc agctacaagc tgaatgaaac ggtgaacgtg acggggaaag
    41101 ccttgaatta cagcggcagt gccgtcgacc aggcttcggt ttcctacagg gtggtcagaa
    41161 cggcccgctt cccggtgtgg cgctcctggt ggcgctggtt cccgcccact cccgaaacgg
    41221 agatcaccag cggcaagacc ctgaccgggg ccgacggatc gttcagcctg tcgttcaagg
    41281 cgttgcccga tcttacggtg gacaggaaat accagccggt tttcaattat acggtttatg
    41341 ttgatgtgac cgatataacg ggcgaggtgc gctcagctga agggagcctg tcggtcggct
    41401 accaggcgat gctcctcgat gccggaatcc ctgacgaagt ggaccagctc ggcccggatg
    41461 cattcccgct cagcgcaacg aatctcaacc gacagccggt gcctgccgaa gtgaaagtgt
    41521 cggtgcatct gctggatgcc cctgcacgcc tcatccggaa acaacaatgg gaaaatcccg
    41581 atgtgtttac catgacccgc gaagaattcc tggccagttt tccctatgag ctttatgatg
    41641 atgaaaatga tcccgaaaca tggaataaaa aggaattgat gatggaaaaa tcgtaccgga
    41701 caccatccga ttcaaccatc atcataaata atttaaaatc ctggaaagaa ggtcattacg
    41761 tgttgaccat cgaatcaaaa gatgcttatg gcgaaaaagt cgaaacaaag aaatttttta
    41821 ccctgttcaa cccctcgctg aaatcgcttc catcgaacga ttcattctgg tattccgtga
    41881 taaaaccagg cggtgaaccg ggcgacacgg ttgcttttac tgccggaacc gccgaacagg
    41941 gggtcaggct cctatacgag attgagaacg acgggaagat catcagccgg gaatggatct
    42001 ccttaagccg ggaaaagaga accttccgga tcccggttaa ggaagaatac cggggaaatt
    42061 tcttcgtcaa ccttgtcttc gttaaaggga acaggagcta tggtgtttcg gaacaattca
    42121 ccgtgccgtt taccgataaa cagcttaaaa tcactgcaga gaccttccgg gacaaactta
    42181 cgcccggaca ggaagaggaa tggaagctca ggattacggg tatgaatggc gacaaagtgg
    42241 ctgccgaact gctggcttcg atgtatgacg cttcgctcga cgctttccgg ggccactcct
    42301 ggtcattcgg cctgtaccct gttcgaagtt cagcagccgg atggtcggtg ttcgatgcct
    42361 tcacccaggc cagcagccat ctcgttaaaa aacagccggc cgatccacct tcctatattt
    42421 ttcatagtta cgaccgtttg aactggttcg gtttcaacta ctacggtgga atttatcccc
    42481 ggatgggcgg aatggaaatg cggatgaaaa acgcaatgcc agccatggac ggaatggcgg
    42541 ccgataaggc tcaggaagaa gctgaaatga cggtgaccga aagcgggaat attccggcag
    42601 aacctttgcc cgatgagaaa aaacccgagg ttccggatat gcccgtcagg aagaatttca
    42661 acgaaacggc attctttttc cctgatctga agaccgatga aaaaggcgat gtcatcctga
    42721 gctttaccgt tcccgaatcg ctcaccgcgt ggaagctcat ggccctggcg tatacgaagg
    42781 acctgaagac tggctacctt gaaaaagagg tggccacccg caaagagctg atggtgatga
    42841 ccaatgcgcc gcgcttcttc cgggagggcg accaggtgat gtttgccgca aaactggtga
    42901 gcatgtcgga taaaaaactt gccgggaaga tccaggctga atttttcgat gcctatacca
    42961 tgataccggt cgacgcgctg gtgaagaacc tggtgaaggt tgttgatttc tcggtcgaaa
    43021 agggcaaaag cgaagttttt ttctgggata tagtcatccc cgaagggctt gatgcattgg
    43081 tctgccgggt gaaagcttcc gcagatgaat tttccgacgg ggaggagatc gtcgtgccgg
    43141 ttttgcccaa ccggatgctg gtaacggaga cattgccgct gccggtgaat ggcaaaggga
    43201 cgaagaactt taagttcagc aagctggcct catccggaaa gtcaggatcc ctcagcagct
    43261 accggctgac gctcgaattt acctccaatc cggcctggta cgccgtccag gcgcttccct
    43321 acatgaccgg gtatgtgcat gaaagcgccg acgggctgtt cacccgctat tatgccaaca
    43381 gcctggcctc ttacatagcc aattccaacc cgaagatcaa agccgtcttc gaaagctgga
    43441 aaaatatcac acccgatgca ttgctgtcga acctggagaa gaaccaggag ttgaaagcgg
    43501 ttctgctgaa cgaaacgccg tgggtgatgg aagcgaaaag cgagtcagaa cgcaagaaaa
    43561 gaattgccgt cctgttcgat cttaaccgga tggctgccga gcagcagtcg gcgctggcga
    43621 agctgcagca gatgcagact cccaatggag gctgggcctg gttcaaaggg atgcgcgacg
    43681 accgctacgt aacacaactg atcgtcaccg gaatcggccg cctgcagcac ctgggcgtca
    43741 tcgaactgca gcgcaatccc gacctgatca gcatgaccca gagcgcattt cgctacctgt
    43801 gcgatagatt gaaggaggat tacgacgaca ttaaaaagga tagcaagggg cggatggatg
    43861 agaaccacct gggcagcgcc cagatccagt acctctatgc gtacagctac ctgatggaat
    43921 ttgtgaaagt gcacccctcc aataaagagg cgttcgacta ttttacgtct caggccggga
    43981 aatactggaa ggatcagaac aagtacctcc agggtatgat ggctattgcc ctgtcgagga
    44041 tgaatgtgcc cgatgtgccg gcagcaatcc tggcgtcgct gaaggaaaac gcgctggttt
    44101 cggaagagat gggaatgtac tggaaagtgc cggaaggcta tttctggtac gaggcgccgg
    44161 tcgagcggca ggcgatgctg atcgaagcct ttgcggaggt aacgcgtgat gaggaggccg
    44221 tggagctgat gaagatctgg ctgctgaagc agaaacagac gcaggactgg aagacgtcgc
    44281 gggctacggc cgatgcggtc tatgccttgc tgctgaaagg caccgacctg ctggcctccg
    44341 accagctggt tgaggtaacg ctgggcacag agaaaatcga tccgctgacc atggatggtg
    44401 tgacggtcca ggccgggacc gggtatttcc aggtctctaa gatggggaag gagataacgc
    44461 cggagatggg aaacataaaa gtggtgaaga aagatgaagg cattgcctgg ggcgctgtct
    44521 actggcagta tttcgagcac ctggataaga tcactccggc cgcgtcgccg ctgagcatcg
    44581 aaagagagct ttaccttgag cggaatacat caaccggccc ggttctcgat cctgtattcg
    44641 acaactcgat cctgaaaaca ggcgacaggg tcaaagccag gatcatcatc cgcgttgacc
    44701 gcgacatgga atatgtccac atgaaagata tgcgggctgc agccttcgag cccattgaag
    44761 ccatttccgg ataccgctgg caggacggtc tcggctatta cgaatcgatc cgcgacgctt
    44821 cggtgaattt ctacttcgat tatctccgca aggggaccta tgtcttcgaa tacccgctga
    44881 atgttacgca gaagggagaa ttttccaacg gcatcacctc gatccagtgc ctgtatgcgc
    44941 cggagtttgc ggcccattcg cagggaatca gggtgaaggt ggaatgattt ggcgatttgg
    45001 cgatttggag atttgaggat ttggagattt gcgggttgga cgattttgaa ttaatattga
    45061 ttatatgaga ttcctgctat cgtttttaat ccttgctgca tctgttttac agggacagaa
    45121 tattgtcagg tatgagaaaa atcatcggga tacggaactt tgcaagataa cgttcattga
    45181 taccaccacc aacgaaatcg taaaaacatt ctcgccggcc gaaaaatgtc catactggaa
    45241 tcttacttat aaaaggatcg gggatacgat caatgaccga agtgtaaaat attttgatct
    45301 gaaaggtatt gattctttga ccggggatga aattcccggc cttattaacg tcactgactt
    45361 tggccatcct ttaaaacaac cttatgaagc ctttacgagt aattcagctc atgtttcaaa
    45421 aaatgggaaa tgggtaatta tttcatcaca tatcattatc gatgatttca gtgatgatga
    45481 aaatgaaagc gaattggaag catacgccga atgcctgcag gtatttgatt caaatggcaa
    45541 tttattccgg aaaatatttt cagatgatgc tgaaatgaat gatgttggta ttactgacga
    45601 tggggattac cttttctatg attaccaaat cttgaaggac ctttattatg ataatcattc
    45661 aaacagaaat cccggtttaa ggattataaa tcttaaaacg atggatatcg aactagacat
    45721 ctctgattat gatacgaatt gggtttacct ctatccttat gtggccggag atttcatttg
    45781 tatcttctct gaaacccgta aagattatga agatgtggat tcaacaggat gcaattcagc
    45841 cgtatgttat aattttaaga agaatataaa gtacacagga cctatagggc ctgcttttaa
    45901 agaattctgg cggattatcg atgtttcgga tgaggggata tactttcggg taagttctgc
    45961 cagggaagca caacccgttc tttacaggtt tgacaaagat ttcacaaaag agaagatcaa
    46021 ataactggca ctcaccaaat cagtgaaaat ccgtataatc cgtgtcatcc gcggtctatt
    46081 aaaaacctga atcctcaatt agtgcatatt ttatgataat tttttaccca attatcattc
    46141 ccaatgaata atattatata tttgtaatga acaataatgt atattaaaaa tgcataaatt
    46201 atgattttaa aggaaactat tgttgagatc cttcaccagc aggatgagaa aataaaggcg
    46261 aaggatgccg gtataccaag acaactgaat gataacattg cagttggtgg aaatctgata
    46321 ttgatcatat ccgggataag acgttgtgga aagagcactt tattgaacca gataaaaaca
    46381 accacacaga aatcctgtta ttttaacttt gaagatatcc tggcaacgga ttttgaatta
    46441 tccgatttta aaaagcttga ttcaattatc aaagaattgt accctggagc ttatcttttt
    46501 ttcgatgaaa tacaggttgt ggaaggctgg gaaaattatg caagatctgc ccaggatgat
    46561 ggtttttcaa taatcattac ggggtcaaac gcttcactgc ttagccgtga attggggaca
    46621 aaactaacag gccggcattt gacgtatgaa ttgtttcctt tttcatatgc agagttcctg
    46681 gtttataaaa aagcaagccc gggcattgat tcattcagcg actatttcat caccggtggc
    46741 tttccggaat atatcaagca tagtaatcct gaaatccttc aacaattact cagggatata
    46801 cttaacaggg atatcatcgt caggcacggg ctgaaaaaca gtaccctggt cgggaaatta
    46861 gcaacttttt taatgaccaa tgccgggaaa aagttttcgt acaactccct tatgaaaact
    46921 ttcgaaacag gctctgccaa tacgattatc gattatgtat cctattttga ggattcttat
    46981 cttttgttga ccattccttc atttagttat tctattaaag atcagatcag aaatccaaaa
    47041 aagatctatt caattgatca tggtatggcc cgtgtcaatt ccctcagttt ttcaaaagat
    47101 gagggaagga tcctggaaaa catggtcttt cttcatcttc gcaggaaatg gaaaaagatt
    47161 tactattatt ccgttaaaag tgaatgtgat tttgtagtat tcgaccgcga taaagtagaa
    47221 atggttgtac aggtatgtta tgaattatca gacgataaca tggcgcgtga aatttccgga
    47281 ttgaaagaag cgatggattt ttttcaattg aaaaccggtt ttttgatcag ctacaaccag
    47341 gaggatgtta ttgaagatgg tgagaggaaa ataattgtca tgccggcatg gaaatggatg
    47401 atggaaagaa aattatgaac ccaaaacact acaccatccc tatcttcatc ccgcagctag
    47461 cctgcccgtt ccagtgcgtt ttctgcgacc aggagaagat cacggcccgg gggcacatac
    47521 ccgggatacc ggaaatggtc aatatcattg agcagtatca tttctcattt cccaaaggga
    47581 agaagcatgt ggaaataggg ttcttcggag ggacctttac cgggataccg cttgaggagc
    47641 aggaaaaata cctgcaggtt gcgggtgagt atattgaaaa aggatgggta cagggcatac
    47701 ggctttccac gcggcctgat tatatagacg aaacgagact ccatctgctc aaaaagtatc
    47761 acgtcaccac gatcgaactc ggcgcccagt cgatggatga tgaggttctg gctttgtctt
    47821 cacggggcca tacggcggag gatattgaga tggcatctca aatgatcatc aaatccgggt
    47881 tccgcctcgg gctccagatg atgatcggac tgcccggcga taccctggaa aaggacgttt
    47941 ccaccgctga gaagatcatc gcactgggcg ctgtggaaac ccggatctat ccgttgctcg
    48001 tcattaaaga tacggtgctt gaaaagtggt accaccaggg caagtaccag cccctgaccc
    48061 tggaagaaac gattgaccgg ctgaagatcc tgctcccgat gttcgaagtt tcgggcgtgg
    48121 aggtaacccg agtcgggctg catgcttcca tcggccttca gaccgggaaa gagctggttg
    48181 cagggcctta tcatccctcc atacgcgaac tggccatgac cggggtctgg tgggacaggc
    48241 tagaggtgtt gactgccttt gaaggtacag atgagcttcg catcacagtt catccttcac
    48301 agtataattt tgccgtcggg tattacggaa aaaaccgcaa aaggctgatg caggtcatta
    48361 aagaggttaa gttcctgaaa gataaagacc tgaatcagat ggagttcctg gtggattaca
    48421 ggtagaggtt caattgttca attgttcaat tgttaaactg ttatattgtc caactgagaa
    48481 atgttgataa tcgttgataa taaactatcc tgtggagcga agaatatact ttcttcctac
    48541 ggagagctca tggaaataca aaccgaaggc atcacctatc ctgctatttc tggtcacccc
    48601 gatattttct tctgccaggg gccggggaag ttgatcgtgg cgccgaatct gccggaacgt
    48661 tattttgatc agctccgtaa gaacgggatc gaatttatca ccggcgagtt gccggtcgga
    48721 ccggagtacc cgtcgtcggc ccgttacaat gctgttgcca caccgaagta cctgatccac
    48781 aattttcgcc acaccgatgt aatgatcaca cgaactttca tggaactgga gccggtctgt
    48841 gtggagcagg ggtactgccg gtgcaacttg ctggcgttga aggatgattg tttcatgacg
    48901 tcggatgagg ggattgagaa ggttttcatg agatggagtc tcggaagaag gagacttcat
    48961 ctcaccaacc ggggacgcca tcccttggga cctcgtcccc agggacgcca tccctacggg
    49021 atcctactcg aaggtttccc ccacggtttc ttcggcggct gctgcggcgt cctggatgat
    49081 aaaatcttca taaacggaaa cctggatttc tttcacgaag gcaaaaaagt cagggatttc
    49141 atttcttcat taaattatga gatcgtcgaa ttgcatcatg gacccctgac agatgccggg
    49201 agtattttgt tttgttaatt catgattatt tatagccggc tgtaatgtaa ttctccagat
    49261 cattatattg accatcagac aataatccct gtgtccaatt gtgatccttc gggcttcgcc
    49321 ctcaggacaa gctgccggct gctgactgcc ggctgccaac tttttcctat cttcgcaccc
    49381 aaattataat ccaacgacta tgcttcgttc ccacacctgc ggcgaactcc gcctgacaga
    49441 taacggtaaa acagtcaccc tcaccggatg ggtgcaacgc tcccgcgacc tcggcggaat
    49501 gacctttatc gacctgcgcg accggtatgg tcttacccaa ctggccttta acctcgaaac
    49561 caacgccgaa ctatgcctcc aggccaggaa gctgggccgt gaattcgtga tccttgccac
    49621 tggaagagtc atcgaacgca gcagcaagaa tcttaagatt cctacgggtg agatcgaaat
    49681 tgccgtcgaa aagctcgaag tgctcaacat atcgaaagtt cctcccttca ccattgaaga
    49741 cgagaccgac ggcggcgaag agctccgcat gcgctaccgc tacctcgacc tgcgccgaaa
    49801 tcccctgcgc cggaacctcg agctgcgcca ccggatggcc atcgaaaccc gtaactacat
    49861 ggactcgcag ggattcctcg aaatcgagac accctacctg atcaaatcca ctcccgaagg
    49921 agcacgcgat ttcgtcgttc cctcccgggt gcataacggc aagttctatg ccctccctca
    49981 gtcgccccag actttcaaac agatcctgat ggtggcggga tacgaccgct actaccagat
    50041 cgtgcgttgt ttccgcgatg aggacctccg cgccgaccgc cagcccgaat tcacccagat
    50101 cgactgcgaa atggccttca tcacccagga agatatcctg aataccttcg aaggcctggc
    50161 acaacatctt ttcaggagag tgaaaaacta cgagaccggc ccgtttaccc gcatgacctt
    50221 tgccgatgcc atgaaattct acggttccga taaacctgat atccgctttg agatgaaatt
    50281 cgccgaatta aacgacctgg tgacaggaaa aggtttcggc gtttttgacc aggcagagct
    50341 gaccgtcggc atttgtgccc cggggtgtgc cgaatactcc cgcaagcagc tcgacgagct
    50401 gaccgaattc gtcaaacgcc cgcagatcgg cgcccgtgga ctggtctata tccaatgcaa
    50461 agccgacggc accttcaaat cgaccatcga taagttctat tcgccggaag acctgaaaaa
    50521 gatcgcggaa gccttccatg ccgggcccaa cgacctgatc ctgatcctcg ccggcgatgc
    50581 gcataaaacg cgtaaggccc tgaatgaact gaggcttgaa atgggcaccc ggcttggact
    50641 gcgcgacccc aatgtcttca aacccttatg ggtcattgac tttccgctgc ttgaatggga
    50701 cgaggaaacg aagcgtttct atgccatgca ccacccgttc acctcgccaa aaccggagga
    50761 tattcctctg ctggaaaccg atccgggcca ggtccgcgcc aatgcctacg acctggtgat
    50821 caacggcgtg gagatcggag gaggttctat ccgtatcttt aacaaggaat tgcagaagaa
    50881 gatgttccag atcctcggct tcagcgatga agacgcccag gagcagttcg gattcctgat
    50941 gaacgctttc gaatatggcg ccccgccgca tggcggcatc gctttcggat tcgaccgctg
    51001 ggtagccatg ttcagcagtg tcgactcgat ccgcgaggtc atcgccttcc ccaaaaacaa
    51061 cgccggccag gacatgatga tcgactcccc ctcggaaatc tccggcgatc aactgaaaga
    51121 gctggggatt accacggttg gcaaataaac ttcaaaattt gaacctaagt aagagaaatt
    51181 cagaagatca ctgttttctt gcccaacaag aattgatttc cggagaataa gctgatatag
    51241 taaactcccg ggggataacc ggaaacatcc accatgattt ctttttcatg gttaacaccg
    51301 gaagtcgacc atatttttcc ggtcatatcc gataccgtaa tttcatcaat gacataagat
    51361 gaagttgaat ttccgctaat cctgacgaat aatctttcat tggccggatt tgggaaaatc
    51421 tgaacctccg ggctttgatg gcctgcaagt cccgtataaa ggccgtcggg agtagatttt
    51481 atgatcatta catctctttc catcatcgta ttattgcgcc agtcgtagta tgttccgagc
    51541 ataacacaac tgccgtcttg cattgcggta atgtcgtaaa ggatatagtt ggcttctcct
    51601 ccgaaacggt tttcccagat aatttcgccg tccttgctga atttaatcac ggcaaaccat
    51661 cggtcgaccg gattgaatcc cttattgggc tccggcatat acgaattttc aatggctccc
    51721 agaaacatcg tatcgatggt caggtaatcg atatcgatgg cctcattctg atcgggacgg
    51781 agatcgaata atgcagtatc ggttgcctga gcaattgtat ctattacaaa ataacccgga
    51841 tcggtcgttg catcccaatt ataaaactgt gacggtataa tgcaatggga actatcgtaa
    51901 ggccggaaaa aaccataact caaaagatcg atcgaaagcg ggtaatagac agaatcaata
    51961 gtaaaatccg tattatcgat gtaacctaac caatggtacc tgccgcataa aatctggtcg
    52021 acagaaggaa ggtaaccgat atttggccat ggacctgcaa aaccaggatt caacctctcc
    52081 tggatgaaat tcccggttcg gctggtcttg accagtaact ggtcccagcc atattcacct
    52141 ttacaagcgg aaaagaccag gttatcctga tcgacagccg tatagcaaaa gaattcaatt
    52201 atactgtcgg atgtgtatgg aatattgatc ccaagaagct gcatattact gttaaaatat
    52261 agtattacca ggctgtatga ttcatcttcc ggccgatagg cattccccca aaccatcagg
    52321 ctatcaccgg ttctgattat acttttaaga agcaggtcat acccgtcaat cttatcaatc
    52381 tcaatatcat ctattatttc tccctggtca ttgatatggt atatggtatt gcggtaatta
    52441 tgaaaaaaat cagcaggtgt gggatccaat tcaaattgac cacgtacgac aattgaataa
    52501 atcccattat ttaaatcaga aagactcccg gcaggaaatt catcttcgaa agtgctgatg
    52561 cgtaatgagt aatcctgaga ataaccgtaa cctggccaat ttgccaaaat aatggtcacc
    52621 agaagtccta atattttata tatattcatg tttaatttgg aaatcctaca taaacaactt
    52681 cgaaatataa aaaatccgca gtatgccaaa tgtcacaacc atgccaataa ccataaggat
    52741 attcaaaaaa attcattatt aaggtaccaa gaataaaatc aggatttgga tatggattat
    52801 attgatccac aacttctttt atggaaagaa gatattgatt acatatcgat gtgttaagca
    52861 tttccaatcc tgatgtgcca ccccagaacc gataatc
//
LOCUS       DAOPNH010000004        33525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_1096_length_33525_cov_7.022826, whole genome shotgun sequence.
ACCESSION   DAOPNH010000004 DAOPNH010000000
VERSION     DAOPNH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_33525_cov_7.022826"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="PKZ74_00670"
     CDS             <1..1733
                     /locus_tag="PKZ74_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPM91289.1"
                     /translation="WDFHYRKEIKLLTHFSFHRLFGETFIVYNPLYQQLKINEAYTVM
                     ADGKKVIVPQNAFNEVLPGYAANIAAYNHLREMVVTHTGTEVGSVITLDYTIHSAGNF
                     LPFFFGMEEIGENVPVKDLRIIVRVPEEIILKERILNSRLAPEVIQLAGQKSYTWSFR
                     DVPALPRSSNQDIEQKTALFFSTAKDMTWAYFSFVNQEAFKSRPGAEISRRVDEVKKD
                     KKSDLDIILALQAVVIDELRPADIPLANSGYKIRTPDEVWKSANATALEKAVLLANML
                     KLANINACPVASVTNAWYADDMGNPAMFDGFMVQVNPKETGRIYLPVTQKQAQNLMFD
                     VSDKTLIQLDGAIESMRTFREKAVENELEMKGELRLYDGKTVGGEVQMVMEGICHPFF
                     KLRKDSSYVKNMITGDLPLSSVRNIKLRKISELSSEYDLTWNPELVPAFVGMTRNPEP
                     GTSNDTGFFYFELPRFKTGFDAWNLVQFTGSGDTPVRLDFPLEEDYSFEIILPESYSL
                     FTPPVDIKIENDLGELAIKISQSGSKVKIDRSWELYHDVVLSDLIDEFKEMIIAWEKV
                     EHRKIILKSR"
     gene            complement(1823..2758)
                     /locus_tag="PKZ74_00675"
     CDS             complement(1823..2758)
                     /locus_tag="PKZ74_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91290.1"
                     /translation="MHTPIKKLSYDELLEILRHYDTESIFEKVNLVPDSEDLHESLAE
                     YINLRSFAKRAVLGIDIYRYGMYKHLEQVLIPPLFKILFEKAIRLCLENNRFVFQHYT
                     KEKIEKSFISTGDGGFVIFDTPLHALFFAINFEMVIRAYNSYHLFPKLRNIIGSLSLR
                     YAISYDTIFTFDNNFYGSAIITNARILNKDTLNRCLIDQQTYDWFLISIDGLENLQVL
                     TIADIANIYDFLDYDRKWIAEGTNEILDTNADNRRTGIINSDVLKVGQIQAKETLVNI
                     FNLHLQISMQIFSDRKVESKRTITVSLGNLNTSGI"
     gene            complement(2957..3532)
                     /locus_tag="PKZ74_00680"
     CDS             complement(2957..3532)
                     /locus_tag="PKZ74_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM91291.1"
                     /translation="MDDHSFLSLVQKRQSVRKYTRKPVEDEKLRRCLEAARLAPSASN
                     SQPWKFIVVNDPELVMKVAKETIGPLSTFNNFVPEAPVILAVVIEKMKVFTKIGASIQ
                     DREYPLIDIGIAAEHFCLQAAEEGLGTCMLGWFNEKPIKELLKIPKERRIGLLITLGY
                     APDDYRLREKIRKKPEEMISFNFYKSHKNTL"
     gene            complement(3707..5014)
                     /locus_tag="PKZ74_00685"
     CDS             complement(3707..5014)
                     /locus_tag="PKZ74_00685"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPM91292.1"
                     /translation="MNQIKIDLAYIVNFISEETINGFQKEIDIHYPALLNKTGKGNDF
                     LGWVDLPLKSDREIFIRIESDVERLRKISEIFVVTGIGGSYLGARAVIEALGHSFYDL
                     LEERKFPYVVYAGHHLSEDYTAELLEILVKKNYSLAVISKSGTTTEPAIAFRILKNHL
                     EKKYGKAGARERIIAITDKQRGALKGLSDKEGYPTYVVPDDVGGRYSVLTPVGLLPIA
                     MAGFDIRKLVQGAADMEAFCRSARTIGENPVMAYVAARNALYRSGRNVEILVNYLPNL
                     VYLTEWWKQLYGESEGKEGKGIFPAGVSFTTDLHSMGQYIQDGMRIIFETVISVENNK
                     KEVAIPLAEDDADGLNYLAGKRIGYVNHMAEKGTVMAHVDGGVPNIRIVVPEISEYVM
                     GQLIYFFELACGLSGYLLGVNPFDQPGVEAYKKNMFELLGKPK"
     gene            5313..6740
                     /locus_tag="PKZ74_00690"
     CDS             5313..6740
                     /locus_tag="PKZ74_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM91293.1"
                     /translation="MKQRTVIFAVIMLSIALTGLMIIQGYWIHNAYKVKHANFIRTVN
                     ESAHSVILVMEKLDLVRKLQQPRLLGGEEFPAVAAVDSINQVLLFEMQKIRTRKDLEV
                     FFNKYFMARELMEDRIFSHSIQDNRQPVDLPLVDSLLSNEFAQRNLNTEWSFGIHNPA
                     SDILVFNDEGIYSAELLNPEIGFHFELYPDDMLDNPDFLLLYFPHERQYLLGQVWPLV
                     LISIILVIIIISSFIYTLIMYFRQNRLSELKTDFINNMTHEFKTPVSTISLACEALND
                     KDIKKSEELYQAYINIINEENKRLGLMAERILQSAALEKGDLVLHREVFDIHDILHEA
                     IRNIGIQVEIKDGQIIKDFQAGRSVIEADRMHLVNIVQNLLDNANKYTPVKPQIIVGT
                     KDAANGIIFTVQDNGIGISKADQKKIFEKLYRVPEGNVHNFKGFGLGLSYVKTVVERH
                     GGYIRLDSELKKGTRFEVFLPDKSK"
     gene            6744..7442
                     /locus_tag="PKZ74_00695"
     CDS             6744..7442
                     /locus_tag="PKZ74_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM91294.1"
                     /translation="MENNELKVLLAEDDKNLGAVLKAYLEAKGYLTTLCINGKEAFDF
                     FNKKKFDFCIVDVMMPVKDGFTLAQEIRKLDKQIPILFLTAKSMQEDKIRGFEIGGDD
                     YLTKPFSMEELLMRMKAILRRVNEKSESEARTSVYKLGKMTFDYNRQILLTASGENKL
                     TSKEAELLRLLCENANDVLDRSVALNRIWQDDSYFNARSMDVYITKLRKYLKEDPSVE
                     LLNVHGIGFKLVLP"
     gene            complement(7495..8766)
                     /locus_tag="PKZ74_00700"
     CDS             complement(7495..8766)
                     /locus_tag="PKZ74_00700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM91295.1"
                     /translation="MIRLIYQFTRYIILPIGAAIGITAFILFVTEEIRESPRYIRKHT
                     FPSDSIFIDLDKPIMARKGHIIDSVFSELQAKGGFNGCILYAEKGRLVFKKAYGYDNF
                     KQKTRLTTSSSFQLASVSKMFTAMAIMILKEDGLLDYDDSVRKFIPELPYSGVTIRHL
                     LNHRSGIPDYMHFADEFWGQEKPMTNEDMIRVMKINAPPRFFSPDSGFDYCNSNYALL
                     ASVVERITVKPFEDFARENIFEPLGMNHTFIYKLDPAKEIPAFVPVGVSGHKAGRSMP
                     RVEPDFYQNGIVGDKGVYSSVEDLFKWDQALFTGQLVDEATLKEAFTPGSPKFSKWRD
                     NYGFGWRLRADRVNTVYHYGWWKGFRTYFIRDLYQEKTIIVLTNTTRSLSSNLLFEIL
                     DDRRYELGPVCPYNPPPKKPRSKDHRQATND"
     gene            8890..9072
                     /locus_tag="PKZ74_00705"
     CDS             8890..9072
                     /locus_tag="PKZ74_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91296.1"
                     /translation="MKDRVQNNASSGAVYGLGFIGAAIYYISTATGFWVGVLGFLKAL
                     VWPAFLVYEALKHLAM"
     gene            9151..10638
                     /locus_tag="PKZ74_00710"
     CDS             9151..10638
                     /locus_tag="PKZ74_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91297.1"
                     /translation="MSEKSFGRSFLLLFLSFLIILVYQVLYDHYPVFGEKYRNFAFFE
                     GWVNCSPADSSSAAKDSAALTVAIDTSLYHAAKDSASMPFTGPDSGSVAADLPARLDH
                     FATALHELKSGKRKKVRIAWFGDSMIEGDLIVMTLRNAIQKEFGGEGVGMVPMTSAVA
                     GFRITVKQTFSDNWQEWNVVEQKPPRFPQGLFCTVHTGDVSTSGDPFWVKFKSSNTFK
                     TTSVFHNASLFYGTPSMLRSGREKSMLATEPKAEVYNGDRLIGSFTLDTVGGLQKIDL
                     FTGPLSEFSVYLNSDSAVPYYGISIESDSGVFVDNFASRGNSGIGLWQIDDNMLWRFH
                     DLLRYDLVILQFGTNAMSPGMTDYNWYGHSMNKVLAKLKKGFSGSSFLLISVADRAMK
                     GDNGMQTDPGVPEMVGVQRRIAAKNGMEFLDLYSAMGGEGTIVKWSELEEPLANKDYT
                     HVNFKGARKISDIILNFLLQAYREYEPETAPAINDSLQPETDTLK"
     gene            10635..12098
                     /locus_tag="PKZ74_00715"
     CDS             10635..12098
                     /locus_tag="PKZ74_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPM91298.1"
                     /translation="MRIGLVAISFLFAVRIFSQVTLVPAGEYAKIHPFLRPDSNVIIH
                     PEALEQFFIKLACLEHDTLTRLRILHIGDSHIQADFFSGPVRKILQQQFGNAGRGLIF
                     PYKIANTNGPSDIDVFSNTSWTPKRNVFPKIDMPVGISGISLKTGNPFAVMRVSLKAV
                     DSVAQPYDQMTVYAHCSRRSFDLAVSLNASSGVLEKSAVYSENTYYTVKSGDNLGLIA
                     RKFGTTVSQLKAWNGLHSDFIRAGQKLIIKKERQAVQAMSLESFTMLDTLFFSDTLQG
                     QRGRVIRFDQPEKTCYLHMVKTDSIQEEFVLQGILLENADQPGILYSMSGVNGAMYEH
                     YNLSSDFLQQVRDYKPDLVILSLGTNETLGSYFDEEKTQIEMRRMIDSLRNLEPAPDI
                     LLTAPSDNMLRRRYPNKSVIRINELMAKEADSCHVALWNLFSVMGGHGSIDKWYKAGL
                     ARADRIHFTVAGYELQAALLMDALMEAYHKFKDNGLE"
     gene            12085..13545
                     /locus_tag="PKZ74_00720"
     CDS             12085..13545
                     /locus_tag="PKZ74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPM91299.1"
                     /translation="MDWNKIIGVFRYDPASPMLFNSGIFLVMFLGFYGIYVLIRDRRT
                     FLFIYTVLFSLYFYYKASGFYVAIFIASTIINFWLGNRIFLTGNKTGKKAWLVSGIIL
                     NLALLFYFKYTNLFLRSWGTISGSEVTHFDIFLPIGISFFTFQSISYLVDIYKKLLRP
                     PENLLNFSFYATFFPHLVAGPIVRAYVFLPQIYQKLKLTARQIGQAWWLIIAGLIKKA
                     VISDYISINFVDRVFDNPALYSGFENLMAVYGYAIQIYCDFSGYSDIAIGLALLMGFR
                     LPINFNSPYKATSVTDFWRRWHITLSTWLRDYLYIPMGGNRKGKLRTYGNLLMTMLIG
                     GLWHGASWKFVVWGGLHGTALAVEKLFQPLIGDRKSAWLKVAGWILTFHFVCLGWIFF
                     RAADYSIALAVIKAIAGFSGWDIALEIITAEWPVFLVLALGYAGHFFSRRYKLKLEEI
                     FSKSPLPVQALAVSLVIWIILQVASAQVQPFIYFQF"
     gene            complement(13596..14390)
                     /locus_tag="PKZ74_00725"
     CDS             complement(13596..14390)
                     /locus_tag="PKZ74_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91300.1"
                     /translation="MAKTSKKPVKNRKPAKKKSRSSGRKKSRISKISLGFAAFIILLM
                     AIWIWNPMLYVNLSDSVKRYYRYMTAEESVTYDETAPEAVLRTQKNYGAEIDKLAAQF
                     DLSPEFLKALIILECSGLKNVKPRFERHIYKRLQNVKHGKLARLENIVPADLNNASDD
                     ALKNMAKSWGPFQLMGYKCIWLDIPLSELRGDKALYWGVKWIDMTYGDYIRRGQYKDA
                     FHIHNTGRPYPESGPPKTYDPKYVPNGLMYMKYFKDYAIPVGGKGL"
     gene            14735..21682
                     /locus_tag="PKZ74_00730"
     CDS             14735..21682
                     /locus_tag="PKZ74_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91301.1"
                     /translation="MKRFVSYILLFLLFSGYIATAQGEDIIIDETNLSGTIGPGSYIT
                     DHNLFVDYTDCLTVLPGTTIKFISNTSLTVYGCLISIGTESSGIIYKSSATSVKWNGI
                     SFPGSKALASDPDDILQSKFKYSHFENVTGPGAIYFSNGNAERTLDSISYCSFTGVVN
                     AIHILFINQAGFVINNCDFSDVSNTGITFGNSPAVSGLQILNNTFSDLSGRFLYVYSN
                     SALESLTVKGNTLDNLVLKKSAIRIENNQYLNALTLESNDYSNFITDVTYPVADGIIY
                     IKGQNILNQFRLDDNTIQTILSSQQLNPPVSSFLYLDHAANAYFKLDNVVSVSSLNDF
                     ILVEDCETVRVEDSKFESLECSGALFNLSADKYCRVEDGIFKDISTNDGNGCFYFSQV
                     ASMDEILFRNNRFEAVTAENGPCLYVNSPSGSVDSVVFRNNDIFQDRMGYTAINGGVA
                     YLRLSSVSLFRSYDNEIRNCEVTGYGGAYCFDVGTVNNLDIYNDDLHTIISNYDGGYI
                     SVKNQITNTDISINGLTADTCFSKAGSGGILYLKSQNNNTGKVRIQNNHLNHFYARSE
                     SQQFAGGGFFIRGSHKELHFTGNALIPNPTPDQIPIADDGGVLYYAHTGSVLDSAIFS
                     GNSFIKADSVAITGSGGAVYLTSTGQTGRIRISDNEFDRFSTAGSGGVFWIGFEGQCN
                     RLDILNNEFSGCVADSSGGAIFFRSSGLNVLNVAGQPGENQLFTDCRARFASGGSLYV
                     LTQAQSIGEINFNHNSFNSPGSAQNALSDGGAVYVKSAGSLVSHLNLSENSVNENNMD
                     IRAGGNGGAFYFNLMGNCTEVNLLNNSLSSCLAGRSGGAVYLRTGNIGRLTVEGETAS
                     PQVKDCHAANGGAYYFDLSGKCDTLDITSNNFMNCAADTTGGAIYFKSNGIGLLNISA
                     QDGDQQKFNNCQAASGYGGVMYFNSLSQGIGEIGIRNNQILSSLNENNSGKDGGGFYF
                     KSVGSDVANIDIRNNTVGNSNKGLKAGMNGGAFYFDLSGKCNQLSIHQNNFNNCRAVN
                     GSGGSIFFLSGGAGTVSIDTNMLLSSSAIENASANGGGIYIKTNGGNVTQISLNGNEA
                     GDDSFGISSGENGGAFSFDLQGTTTLLAVTGNRFVKCRAQDSGGGLYFKANGLNELKL
                     QGFEDELQHFSGCSAADGSGGAVCITTTSQPVSYAYIEYNTVLPAAGMVNAGENGGGI
                     SITSAGNLVEECFVDHNIFGSASGSLKAGNSGGAISVDISGQCNTLRLFENSFTGCQA
                     VNNGGAVSIHAGSLGTLTVSGSPDQYQVFGDCSTENLGGCFYLESDGVGHADFQHLKF
                     ENSTAESGGALAFSSPDMDTLSLNTIEFTDNSASDFGGSIYLKGDGNNMPTIDVLEMN
                     KCTFINSEADNGDGGALFFKGNLGLFNCSSNSFNQCEANGANSGSGSAMYLELSDDFT
                     NELVFTSDTVKNCHSVSGGIFLKNIDKATFLEGHLVGNTATQDGAAIYIQDFDTVLIT
                     GGLFSQNESQQTGGAILLKAGNSESSCCDIGSSEFEFNKALNGGAVTLLDFNQSSLLN
                     NRFIQNSAFSQSMECSGGAVYTVDIDDLVFDGNEFISNSASTTSTQNNIQSYGGSLCI
                     LNNKYFEGSGNDFRFSNAEVGGAVFAKNTDATLNPSVKYDNNFFYNNGSTFGGAVYYD
                     LMNKGGIYATENVFLMNGAGFSGGAIDLRNIGRFESVRNIFRSNFNEGKNLSGVVGAS
                     AILTWNVPESNYYNTVFDYNFYEKDTITERESTIWIKNHDNVYFENCTFLNQIPQDNP
                     VIYKDQNSEPLTIVNSIFSKNVTPETPLVNDFAEIRHSYIEGLITEKIDTMNCFRYPP
                     PQFVDGTYNLNDNDFAFIDAGIPEAVYNDSIDYPIGKGTETCDLGVSGGRYNLWDEEQ
                     LSIPLANQYSRTIIIERYNGECGRYRIYFALNEGDEFTDYTWYIFDEIHKTGSVNEFI
                     GYFPQDQLIAVTGVASNESEGITVIGNNTFSTFYEITLDGSQISYEGSDYQCGATIEL
                     NDNCPVQLNNIVLHLEGLDMPFFSSYKFGWSFRDAIGIASQNITDQSYEEVVFSLDME
                     ADTTSLRSVTVVYNGYDTVCDITFEYECLIRFHYSNLENVLELVEIAPPDMILDPGNV
                     MFDVKLNDPAYYCNGVDFYLIEEGSVATSLDFIRLRSVATGNTVPIQGITYAGNIFQL
                     TFNVTDLPKGEYDVEMQNLCNSCGFPFTLVNRIDIKFEGIAGMADSELKIWPVPANEK
                     IFIENEKTASVMRFSLVDPLGEILFTDNIYPGKRYELNTGRYPGGIYILIFEPFDSKI
                     KSYRKIIIE"
     gene            21687..23444
                     /locus_tag="PKZ74_00735"
     CDS             21687..23444
                     /locus_tag="PKZ74_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91302.1"
                     /translation="MKKKSLLLIFSLFTLFGFCQQEVFWFPAKGRVYGDVMRIMDDNN
                     PYVRNRIQGFNEGDINVNPEYWFNGFKAPANDFEWSNQSGVYYSQLDYPSILADKDVT
                     TIILDFVTVWDEQFYDTAEYYYSKPVMECLTVNNGSYSENMLLVNPTEVTLDFRLYAS
                     LFNLPAPTDGDDRIDGIVEVIDGNVKPINIADTRNGTYIHGVDLSSLSYGLHELEYYA
                     YGQKSDVNLPDEFSDKIKIRILIFDFKLRYDEDYAVCKCDTAYDLEGRPEGGIFSGEC
                     VVGSSNVFNPELTQNASTLITYKYPVDGMYYNVERTVSFDPLPVIAVDATTLAGFPHE
                     ACGFEHGAVYEVTGTGFEDLFRLPPLGIIKQFHYDGNKLTIDWKGEGDGSIYVTALSD
                     KGCSASLEHMVHITFNRAPEDSAYVTLYDRMLFCNADTASVKVFDWYGVIGGSNVFYE
                     RTTNPYYVLDFIPAAGDSFYVLTADDTTSCSTHSHIYTKPAVPKSDLIGEGAEQPAVK
                     LFPNPVRGDVNCEILKPVKEGRIIITDVLGRQISRINHDGFLPGEVVRLETGDFKSGI
                     YYVVFSGNNLSGSYKFIVY"
     gene            23460..25064
                     /locus_tag="PKZ74_00740"
     CDS             23460..25064
                     /locus_tag="PKZ74_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91303.1"
                     /translation="MMDNRVKYTLFILLTFAALNGFSQKIPAKLEAEVSDQVSETIAK
                     FTRQISSIYTSAGETSFRFDSVALNRTRTMFYKYNDPSFKHISPNIIDPSKEFGLSSF
                     PKDTNILPENFIVPIDQFLDSLAAWYCNDSCITTLELGYMDPKTDWNNYYIDPQLLCL
                     QVFYKFNLFGEPYIPRYNEIVARQQITKDQNAAFDDLFEEDAGFAGQLDTRTTVIFLF
                     LLDNYKIKETYIYKIYDSFIDKSVFIEDVRQITSERNKYYLSIRPYIGTSVLKADFSN
                     INNFEQTEEFQPYLGVHIDFNYYFNDGPRRFTKGLSMGAGWSRFNATGKVFDYNASFT
                     EFSEPFDQNYTKYVYADNIHENLMLDYADVNLLIKLRYRLFKNTELIGSIGPQASFLI
                     NSKIEPEAGSEGFVNYEGYFTDIEFPNGTSGEFLLQNVPEYGFTKYTDLKFSENEIGI
                     NKFNFCAYADIGLNLKMSHKFHLSFGIGYMYGFGNLVSESSGEEFVLSKGEGEVDNLF
                     KNCTSLKISAPSAFVSAAYLINTKIK"
     gene            25074..26204
                     /locus_tag="PKZ74_00745"
     CDS             25074..26204
                     /locus_tag="PKZ74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM91304.1"
                     /translation="MKQFILSLFFLATSVMLFGQEEQCLFRTQPQDTETCLNGLAVFD
                     AGVDTLFFENSFFFEWQYKPAGAADWIPVQNNGTFQVTNTQLSSMLTVTINGDASLDG
                     ADFRCSVSDLYYSESASMVIHSLPVVSFTSQNYCFGDITGFTNTSPDAASMKSWAWEF
                     SDGSKYISRDINHRFLLPGDYKVKLTLTNPSDCEAADSAVVTILPLPEPVIKYTKDVF
                     CGFESNLTFYTEDIFESYLWEIRGANNVLASEETEITFDCDENFPTGQYEAILMVTDA
                     NGCSADVRKSFLVLSSQAPVDGTVLQKEDDSRLLVLLIDHQENSDFLWMKMDPVTKTV
                     LEQYKTKEPYHLFGEPIDKNSFEYGVEVIPPFSDCSAVFYLN"
     gene            26437..28734
                     /locus_tag="PKZ74_00750"
     CDS             26437..28734
                     /locus_tag="PKZ74_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91305.1"
                     /translation="MPTNRFSAAAAEKFTNFFLSKETLIFNDIAQDEDMSLYITPAEY
                     IKKVSELYPIGLYVYISDAEIIDVREYSSYILYIVEIEKTIYIDNMLRTNRPVPKFNS
                     PVRQIYYIIGYSNSPGIKIIGIEAKGTAADNKTAYNGLVPDKLEFNAHFNAYNLNFKD
                     IREEYAGLDGSGNQTRFGLLLGWDLGGKKSFRYGLASGLLYDKVNFSTDLESFADRPI
                     PAVDIDNYPYRKIITGHDISQSNSLDQLTLPVLFSMNWEFPPKWGNKYVKRSFTGDLA
                     YKRGIGINFRIGPHIQYNLSGKSDPYEGTFSYGGSYRFFNPVVQDSTTIIIDNLPEYG
                     FVNDTTFTDNSSVASYSKINIGFSTQLNVSIPIYKSLEFYFGPSLYYNFSGLAETTED
                     FILSGRIGEINPFITAAKTSSLSYGVNAGLVLNLHAPRVPYSSIELPDIAKDRYKVKT
                     AYTLENDNRMKMALNVNLIAGKSMEMERQAINYQIQSDWAKKPTKGKFAGFKTHILKY
                     EYPKIAGKVTFKSDLVIEKPFGYDVVCNDTIAFNSVNTNPLVIPLDNLNRLAENRQSL
                     DLTVNRLPDFTFVYVSLYNKFETLEQRRKIVELVRDLGNNAIINQEELLVYLSSEAGK
                     PIAYCNFASREDLPENVVTDWQEFLYKLKEEYGSQNLIYEDDIRNIDNILNPILNLDE
                     RMNAQRRFVNFNFIVNDPEKYYGYDSERLSDTRALIHFLSDKYCSKIPFRSSQFDFNV
                     YLTERNLNMRKSPSDININIIHLIK"
     gene            28745..31276
                     /gene="tssR"
                     /locus_tag="PKZ74_00755"
     CDS             28745..31276
                     /gene="tssR"
                     /locus_tag="PKZ74_00755"
                     /inference="COORDINATES: protein motif:HMM:NF036394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssR"
                     /protein_id="HPM91306.1"
                     /translation="MKKYISQLLILSSFFLSLQMVSGQELIRKPNSELINSVNEIIGK
                     TSYERGTTRWFVYSNRKGNIVYSDAGSVISSNVKFLETFNVVAEDNNRLKVKKLDGEV
                     VGWMDKDDLIILDHCERKANMVSKKAMIINTIDRASANANRDSSQFRFYFDPDLTKRN
                     PKEQSSVYEILYVYKESGNALLLGRREDCSVDVEAYLKESIIGWIAKESVTEWDSRGC
                     LLPNYKPEAKTERRTQGTQPAILDTSSRYTDYSLAKAYDSVLLTYANLKGYAETSFDR
                     SPIVWNEEIDNRWPPSKFRLPYLDKHNNDIDIMKVGVLGDITDAQGRQIQALIDNLRE
                     WERQNRTKNSLNLVFVIDGTYSMKNYFPAVANAISESHKIISKDPDFSDPDNPNRVTV
                     KCGAVVYRDLAEGEYLVETSPLQDFNATRNWISSNFSDMKNRNDRDIPEAVFNGLYYA
                     IEDVLVGHEEENNLIILIGDAGDRNDPEDEVYIEIDSLVYGLYKNNCNFIAYQTHWKN
                     PQENPDNISYTEFCEQMSTISERSAQMIQKKIQGIRNLDLEGSVRLVQVESTSSYNLY
                     ELDQPFLANKVYCNKGSQIDAARLTEFIIASIKASSKNVSLVNKFIHEILYSGMSWDE
                     ILRKYNISQDNAANLGPIATTLGYIVGELKKIYPREDVTAPGGRLEQLLQGKLQLYFE
                     GFTPLRINNSNNNLWTFEILTIAQTKKNLERDYMQVINAFRNQGSADQRRQLIYNAFM
                     TLARTYAGDETNYENLTIGVLFAKIIDCPGLKFSSRYRDCLSKTIRQIRLEQECPDEC
                     LDVFYTEIYRSYNRITNLSYKDYVTTAFGTFSAYYIPVELMPFVN"
     gene            31309..>33525
                     /locus_tag="PKZ74_00760"
     CDS             31309..>33525
                     /locus_tag="PKZ74_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM91307.1"
                     /translation="MNNSIEIKNASFVYKTKISKKETIEREVLHVRNLEIKQGEITFI
                     IGRSGSGKSTLLETLGMMNDTFPVDTKEMEINFHLKKDEEPLNIFKKKIWEKKKDTEA
                     LLNQIRLNNFSFIFQNTNLLNNFTAIDNVCITDMLNGNSYSEAYLKSLIIFSLLGLGD
                     LDVGIKPQHISGGQRQRVAFARAIIPDYQFLFGDEPTGNLDKETSTEVLQLLSDFIRG
                     TNSETSTIIVSHDIDLALKFADRIIVITKEDMGDIKPENVFYRGEDHQKKSGPAKSYI
                     FKSKIHKAFKDNPELIEGFKILVQDAVQQLEKESKSEFKIKPGFFQKMYSLFEAKEVR
                     FSEIRNIKVNSLLRKILFLMQNPEQRSAGIRGLNFFDETFFNELESILEAKGTNLAAK
                     NGEESEKAEAKSWRRKTDSGEKALGDGELRSMLSVLLKFFEREDKLAVSGSTKSIYKN
                     ENSFFFFLFISISRLLSRIITLKNDNLATPNADINNPDFARLFIRNEGGILLGAGKKL
                     THFRTLFFALFVTFLGIGFATGSLSYLKDKMDDPFVNFVTTSVPSEKQSEVASIIQHY
                     NRESELKNQYKINVFTGFTIFPVEIYNKKNNDLSLITGRTITLNDPLLKELFKDKDNI
                     ITGNETGLFNDYDCGFIVSESLIKKLGMEPDARFLKVKILTTNTSAPDTASTGEIAFN
                     EVTDNYELVPVPIRAILKKIPGDLNQDIDFIITEYAHLNLLDNHEPSKEYPFHPSHT"
BASE COUNT         9076 a         7741 c         7866 g         8842 t
ORIGIN      
        1 cctgggattt ccattaccgc aaggagatca aactgctcac ccatttttca ttccaccgcc
       61 tcttcggcga gacgtttatt gtttacaacc cgctatacca gcagttgaag atcaacgaag
      121 cctacaccgt gatggccgac gggaaaaagg tgatcgtgcc gcagaacgcc ttcaatgagg
      181 tgctgcccgg atatgcagcg aatatcgcgg catataacca cctccgcgaa atggtggtga
      241 cacacaccgg cacggaggtc ggcagtgtca taacactcga ctatacgatc cattccgccg
      301 ggaactttct cccgttcttc ttcgggatgg aagagatcgg ggagaatgtc ccggttaaag
      361 acctgcggat catcgtgagg gttcccgaag agatcatcct gaaagaaagg atactgaaca
      421 gccgcctggc ccccgaggtc attcagctcg caggacagaa aagctatacc tggtcgttcc
      481 gcgatgtgcc ggcgcttccc cgcagcagta accaggatat tgaacaaaaa accgccctgt
      541 tcttttcgac cgcaaaggat atgacctggg cctatttctc gtttgtcaac caggaggctt
      601 ttaaaagcag gccgggcgcc gaaatatcca ggcgtgtgga tgaagtgaag aaggataaaa
      661 agagcgacct cgacatcatc ctggcattac aggcagtagt tatcgacgaa ctcaggccgg
      721 ccgatattcc gctggccaat agcggctata agatccgaac ccccgacgag gtgtggaaaa
      781 gcgccaatgc gaccgccctt gaaaaagcgg tcctcctggc caatatgctt aagctggcca
      841 atatcaatgc ctgcccggtg gcatcggtca cgaatgcctg gtatgccgac gatatgggaa
      901 atcctgccat gttcgacggt ttcatggtgc aggtcaatcc gaaggaaacc ggcaggatct
      961 atctcccggt aacgcagaag caggcacaaa acctgatgtt cgatgtcagc gataaaaccc
     1021 tgatccagct cgatggcgcc atcgaatcga tgcggacgtt cagggaaaaa gcggtggaga
     1081 atgaattgga gatgaaaggg gagttgagac tttatgacgg taagacggta ggaggggagg
     1141 tgcaaatggt tatggaaggg atttgccatc cgtttttcaa attgagaaaa gattcgtcgt
     1201 atgtgaagaa catgattacc ggggatttac cgctatcctc cgtcaggaat atcaaattga
     1261 gaaaaatctc cgaactctct tcggaatatg atctcacctg gaacccggaa cttgttcccg
     1321 ctttcgtggg aatgacgcga aacccggaac ccggaacttc aaacgatacc gggttcttct
     1381 actttgagct tccccgcttc aaaaccggct tcgatgcctg gaacctggtg cagttcaccg
     1441 ggtcgggcga tacgcctgta aggcttgatt ttccgctgga agaggattac agttttgaga
     1501 tcatcctgcc cgaaagctac agtttgttta cccctccggt ggatataaag atcgagaacg
     1561 atctgggcga actggctatt aaaatcagcc agtcgggttc aaaagttaaa atagatcgtt
     1621 cctgggagct ttatcatgat gtcgttttat ccgatcttat agatgaattc aaagaaatga
     1681 tcatcgcctg ggaaaaagtc gaacaccgta aaatcattct gaaaagccgt taaaaaccgt
     1741 gttaattcgt ctaattcgtg taattcgtgt tctattgaga tgatagcaca cgaatgatac
     1801 gaattttacg aatattcacg gattatatac cggaggtatt aagattaccc aaactgacgg
     1861 tgatcgtccg cttgctctcc actttccggt cggaaaagat ctgcatcgat atctgcaaat
     1921 ggaggttgaa aatgttgacc agtgtctcct tcgcctgaat ctgcccgact ttcagaacat
     1981 cggaattgat gatgcctgta cgacgattgt cggcattcgt gtcaaggatc tcattcgtcc
     2041 cttctgcaat ccatttccgg tcataatcca ggaaatcgta aatattggcg atgtcagcaa
     2101 tggtaaggac ctgcaggttc tccagcccgt cgatgctgat caggaaccag tcgtaggtct
     2161 gctggtcgat caggcaccgg ttaagggtgt ctttgttcag gatacgggca ttggtaatga
     2221 tggcgctgcc gtagaaatta ttatcgaagg tgaaaatggt atcgtagctg atggcatagc
     2281 gcaggctcag gctgccgatg atgttccgca gcttcgggaa tagatgatag gaattgtaag
     2341 cccggatcac catttcaaaa ttgatggcga agaacagagc gtgcagcggg gtgtcgaaaa
     2401 tgacaaaacc tccgtcgccg gtgctgatga acgatttttc gatcttctcc ttcgtgtagt
     2461 gctggaatac aaaccggtta ttttccaggc aaagcctgat ggctttctca aacaggatct
     2521 tgaacagcgg cgggatcagg acctgctcaa ggtgcttgta catcccgtac cggtatatgt
     2581 cgataccgag aacggcccgt ttggcaaaag agcgcaggtt gatatattcg gcgagcgact
     2641 cgtggaggtc ttcgctgtcg ggcaccaggt tcaccttttc gaagatcgat tcggtgtcgt
     2701 aatggcgaag gatctccaat agttcatcat aggagagttt cttgatcggg gtgtgcattg
     2761 ggagttaaga ttttttgcaa agatagaaaa agagttccag gttccaggtt tcgcgtcatt
     2821 cccgcgaaag cgggaacagg ttccgggttt caggtttcgc gtctttcccg cgaaagcggg
     2881 aaccagttcc gggtttcgca tagaagtcga tgagaaacct tcacagtccg acgactaaag
     2941 ccgtcggttc aaacctttat aaagtatttt tgtgggattt gtagaagtta aagctaatca
     3001 tttcttcggg ttttttcctg atcttttctc tcaaccggta atcatccggg gcatatccga
     3061 gcgtgatcag caatccgatc cgcctctcct tcgggatttt cagcagttct ttgatcggtt
     3121 tttcgttgaa ccatccgagc atgcaggttc cgagcccttc ttcggcggcc tgcaggcaga
     3181 aatgctccgc cgcaatgccg atatcgatca gcgggtattc acggtcctgg atcgaagcgc
     3241 ctattttggt gaagactttc attttttcga tcacaactgc taggatgacg ggagcttcgg
     3301 gcacgaaatt gttgaaggtc gacagcgggc cgatggtctc tttggccact ttcatgacca
     3361 gctcggggtc attcaccaca atgaatttcc acggctggct gttgctggcc gacggcgcca
     3421 ggcgtgccgc ttcgaggcac cgccgcaatt tttcatcctc taccggctta cgggtatatt
     3481 tccgaacgct ttgacgtttt tgtactaatg agagaaaact atgatcatcc atgatatttt
     3541 gtaattgatt ctcttcgtgt aacttcgtgc cttcttcgtg aatcttcgtg tactaaagca
     3601 aggttaaatt gtttcacgaa gttccgcgaa gaagacacga agaaccacga agcattaata
     3661 attttcccgg actaaacact taaccctcaa tccttaacac ataaaactat ttgggtttcc
     3721 caagcaactc gaacatattc ttcttatacg cttccacccc cggctgatcg aaaggattga
     3781 cgccgagcag gtagccgctc agcccgcagg ccagctcgaa gaaatagatc agttgcccca
     3841 tgacatattc gctgatctcg ggtaccacga tccggatatt gggtacgcct ccgtcgacgt
     3901 gtgccatcac cgtccctttt tcggccatgt ggttcacata gccgattcgc ttgccggcca
     3961 ggtagttcag cccgtcggca tcgtcttcgg ccaaaggaat ggcaacctcc tttttattat
     4021 tctccacaga aatgaccgtc tcaaaaatga tgcgcatacc atcctggatg tattgtccca
     4081 tcgagtgcag gtcggtggtg aagctcaccc cggcagggaa aattcctttt ccttccttcc
     4141 cttcgctttc gccgtaaagc tgtttccacc attcggtgag atagaccagg ttcggcaggt
     4201 aatttaccag gatctccaca ttccggccac tcctgtagag agcgttgcgg gctgccacgt
     4261 aggccatcac cgggttttcc ccgatggtcc tggcgctgcg gcaaaatgct tccatgtcgg
     4321 ctgccccctg caccagcttc cggatatcga aaccggccat ggcaatgggc agcaaaccga
     4381 ccggcgtgag gaccgaatac cgtccgccaa catcatccgg aacaacatac gtgggatagc
     4441 cctctttgtc cgacagtcct ttcaacgctc ccctctgctt gtcggtaatg gcgatgatcc
     4501 gctcccgtgc accggctttc ccgtattttt tctcgagatg gttcttcagg atgcggaaag
     4561 cgatggccgg ctcggtcgtg gtgcccgatt tggagattac ggccagcgaa tagttttttt
     4621 taaccaggat ctccagcagt tcggccgtat aatcctcgct caggtgatgt ccggcataca
     4681 cgacatacgg gaatttgcgc tcctccagca ggtcgtaaaa cgaatgcccg agggcttcga
     4741 tcacggcccg cgccccgaga tacgatccgc cgatgcctgt cacgacgaag atctccgaaa
     4801 tcttccgcaa acgctccaca tcgctttcga tccggatgaa tatttccctg tcggatttca
     4861 acggcaggtc gacccacccc aggaaatcgt ttcctttacc ggttttgttc agcaacgcag
     4921 ggtagtgtat atcgatctcc ttttgaaaac cgttgatggt ctcttcggag atgaagttta
     4981 caatgtatgc caggtcgatt tttatttggt tcattttgtt ttgattttgt ttcacaatga
     5041 attatcaaag gatttcactg agggcgcaaa attagcaata ataccgctat aaaaagatga
     5101 agcccgcctg gcagcgggcc gcatccccca ataaaccaaa aaccaaaatt atgaatcaga
     5161 cttctcgcgc aatataaccc agtgattcat ttgtctggat tagcgagaca aaaatatatc
     5221 acaaaatccg ttccaactgt taacgaaatt taaggaaagt taaaatatgt taaaccccct
     5281 tgtatcgcgc tataattcag taattttgac tcatgaaaca acggacggtc atcttcgctg
     5341 ttattatgct ctccatagcc ctgacagggc tcatgatcat ccaggggtac tggatccaca
     5401 atgcctataa agtcaaacat gccaatttta tccggacggt gaacgagtcg gcccattcgg
     5461 tgatactcgt tatggagaaa ctcgatctcg tcaggaagct gcagcaaccg cggctcctgg
     5521 gcggagaaga atttccggcc gtagcggcag tcgattccat taaccaggtt ttgctctttg
     5581 agatgcagaa gatccgaacc cggaaagacc ttgaggtgtt tttcaataag tatttcatgg
     5641 cccgtgaact gatggaagac aggatattct cacatagcat tcaggacaat cggcagccgg
     5701 tggatcttcc gctggtcgat tcgcttctga gcaatgagtt tgcacagcga aaccttaata
     5761 cggaatggag cttcgggatc cataatccgg catcggatat cctggttttt aatgatgaag
     5821 gaatctacag cgccgaattg ctgaatccgg agatcggctt tcattttgag ctttatcccg
     5881 atgatatgct cgacaatccc gattttctgc tgctctattt tcctcacgaa aggcaatacc
     5941 tgctcggaca ggtctggccc ctggtgctga tctccatcat tctggtcatc atcattatct
     6001 cttcctttat ttacaccctg atcatgtatt tcaggcaaaa caggctgtcg gagctgaaaa
     6061 ccgatttcat caacaatatg acccatgaat tcaaaacccc ggtgtcgacg atttcactgg
     6121 cctgtgaggc tttgaatgat aaggacatca aaaagtcgga agaactgtat caagcctaca
     6181 tcaatattat caacgaggaa aataagcgat tgggcttgat ggccgaacgg atcctgcaat
     6241 cggctgctct tgaaaaaggc gatctcgttc tgcacaggga agtgtttgat attcatgaca
     6301 tcttgcatga agcgatccgg aatatcggca tccaggtgga gatcaaagac gggcagatca
     6361 ttaaggattt ccaggctggt cgcagcgtca ttgaagccga ccggatgcac ctggtaaata
     6421 tagtgcagaa ccttctcgac aatgccaaca agtacacacc ggtaaagccc cagatcattg
     6481 tcgggacaaa ggatgcggcg aacgggatca tttttaccgt gcaggataat ggcatcggga
     6541 tcagtaaagc ggatcagaag aagatatttg aaaaattata ccgtgtaccg gagggaaatg
     6601 tccataattt taaaggtttc ggactgggac taagttatgt aaaaacggta gtggagaggc
     6661 acgggggcta tatacggctc gacagcgaac taaaaaaagg aacccggttt gaggtttttc
     6721 ttcccgacaa atccaaatag aaaatggaaa acaacgaact caaagtgtta ctggccgagg
     6781 acgacaagaa ccttggcgcc gtgctgaaag cctatctcga agcaaaaggc tacctgacca
     6841 ccctttgcat caacggcaag gaggcttttg atttttttaa caagaagaaa tttgattttt
     6901 gcatcgtgga tgtcatgatg ccggtaaaag atggatttac gctggcccag gagatcagaa
     6961 agcttgacaa gcagatcccg atactgtttc tgacggccaa atcgatgcag gaagacaaga
     7021 tccggggttt cgagatcggg ggcgatgatt accttaccaa acctttcagc atggaagaat
     7081 tactgatgcg gatgaaagcc atcctgcgaa gggtcaatga aaagagcgaa tcggaagccc
     7141 ggacctcggt gtacaagctg gggaaaatga catttgacta taaccggcag attctactaa
     7201 ccgcctccgg ggaaaataag cttacttcaa aagaagccga actgctccgt ttgctttgcg
     7261 aaaatgccaa cgatgtgctc gaccgaagcg tggccctcaa ccgtatctgg caggacgaca
     7321 gctatttcaa tgcccgtagc atggatgtct acataaccaa attaaggaaa tacctgaagg
     7381 aagacccctc cgtagagctg ttgaatgtgc acgggatcgg ttttaaactg gtgcttccct
     7441 gagcaatctg aaagcagtcg tcagtcgcct gtcctgaggg caaagcccga aggattagtc
     7501 gttagtcgct tgtcgatggt ctttggaccg gggcttcttc ggtggagggt tataaggaca
     7561 gacgggcccg agttcatatc gcctgtcgtc aagaatttca aataaaaggt ttgaagaaag
     7621 cgaccgggtc gtattcgtca acacaataat agtcttttcc tggtaaagat cacggataaa
     7681 ataggtgcga aaacctttcc accagccgta gtgataaact gtattgaccc ggtcggcgcg
     7741 caaacgccat ccgaaaccat aattatcacg ccatttggaa aacttcgggc ttccgggtgt
     7801 aaaggcttct ttaagggttg cttcatcgac aagctggccg gtgaacagcg cctggtccca
     7861 tttgaacagg tcttccacgg aggaatatac ccctttatcg ccgacgatac cgttctggta
     7921 aaagtcaggt tcgaccctcg gcatgctccg cccggccttg tgtcccgaaa caccgacggg
     7981 gacgaaagcc gggatctctt tggccggatc aagtttataa atgaacgtat ggttcatccc
     8041 caggggttca aagatgttct cccgtgcaaa atcctcaaac ggctttacgg taatcctttc
     8101 aacgaccgaa gcaagcaggg cgtagttgga attgcagtaa tcaaatccgc tgtcgggcga
     8161 gaaaaaccgg ggcggtgcat tgattttcat gacccggatc atgtcttcgt tggtcatcgg
     8221 cttttcctgt ccccagaatt catcggcaaa atgcatataa tcggggatac ccgaccggtg
     8281 gttcagcaaa tggcggatgg taacccctga gtagggcagt tccggtataa atttccgcac
     8341 actgtcatca taatccagca gcccatcttc tttcaaaatc atgatggcca ttgccgtgaa
     8401 catcttcgac accgaagcca gttgaaaaga tgaagaggtg gtgagccggg tcttctgctt
     8461 gaaattgtcg tagccgtaag cttttttaaa aacaagccgc cctttttcag cataaagaat
     8521 gcagccgtta aatcctcctt tggcctgcaa ctcactgaat acactgtcga tgatatggcc
     8581 tttccgggcc atgatgggtt tatcgagatc gataaaaata ctgtcggagg gaaatgtgtg
     8641 tttcctgata tacctgggcg attccctgat ttcttccgta acaaacaaaa tgaaggctgt
     8701 gatcccaata gccgccccga tcgggaggat gatgtagcgg gtgaattggt atatcaggcg
     8761 tatcacggat ctttaaaatt gcggcaaaaa tagggctaat cctcaaaaaa aaacttatca
     8821 aacgtacaac tcaaaccaat ttttcttctg aattttacag gctgttacaa atcaattatt
     8881 taaataatca tgaaagacag agtacaaaac aatgcctcat ctggcgccgt ttatggattg
     8941 ggtttcatag gcgcggccat ttattacatt tccacagcta ccggtttctg ggtgggcgtt
     9001 ctgggttttt taaaagccct ggtatggccg gcattcctgg tttatgaagc cctgaagcac
     9061 ctggcgatgt aagagattat tctctctttt caattctttg cttaaatcct gtatttttac
     9121 agcctcaaat ttgcaggcag gtagattttt atgtcagaaa aatcattcgg caggtcattc
     9181 ttgttattat tcctttcatt tttgatcatc ctggtttacc aggtgctata tgaccattac
     9241 ccggttttcg gggaaaaata ccgcaacttt gccttttttg aaggttgggt gaattgtagt
     9301 cctgccgata gcagctcagc ggccaaagat agtgcggccc taaccgtggc catcgataca
     9361 agtctgtatc atgcagcaaa ggattctgcc agcatgccat tcacagggcc tgattccggt
     9421 tcggttgctg cggatttgcc ggccaggctc gaccattttg ccaccgcact gcacgaactg
     9481 aaatcgggca aacgaaaaaa agtgcggatt gcctggttcg gcgattcgat gattgaaggc
     9541 gaccttatcg taatgaccct gcggaatgcc atccagaaag agtttggggg agagggagtg
     9601 ggaatggtgc cgatgacctc ggctgtcgcc ggcttcagga tcacggttaa acagactttc
     9661 agcgacaact ggcaggaatg gaacgtggtc gaacagaaac ctccgcgatt tcctcaaggg
     9721 ctcttctgca ccgttcatac gggtgatgtc agcacaagcg gcgatccctt ctgggtgaaa
     9781 ttcaaatcca gtaacacctt taaaacgacc tccgtttttc acaatgcctc cctgttctac
     9841 ggcacaccat ccatgctccg gtcgggcagg gagaagtcga tgcttgcaac agagccgaaa
     9901 gcggaagtat acaacggcga ccggttgatc ggttcattta cactggatac ggtgggcgga
     9961 ttgcagaaaa tcgatctgtt caccggtccg ctcagcgagt tttccgtcta tctcaattcc
    10021 gacagtgctg taccctatta cggtatcagc attgaaagcg attcgggggt gtttgtcgac
    10081 aatttcgcct cgcgcggtaa ttcgggcata gggctctggc agatcgatga caatatgctc
    10141 tggcgcttcc acgacctgct ccggtacgac ctggtgatcc tgcagttcgg caccaatgcg
    10201 atgtcgcccg gcatgaccga ttacaactgg tacggccatt ccatgaacaa agtgctggct
    10261 aaactgaaga aaggcttttc aggcagttct ttcctgttga tatcggtggc cgaccgggcg
    10321 atgaaaggag ataacggcat gcaaaccgac cccggcgttc ccgaaatggt tggcgtgcag
    10381 cgcaggattg ctgcaaagaa cgggatggag ttcctcgatc tgtattcggc catgggaggg
    10441 gaagggacga tcgtaaaatg gtcggagctg gaagagcccc tggccaataa ggattatacc
    10501 catgtgaatt tcaaaggcgc caggaagatt tccgatatta tcctcaattt cctgcttcag
    10561 gcataccggg agtacgaacc ggaaacggcg cctgcaataa atgatagtct gcaacctgag
    10621 acagataccc tgaaatgagg atcggactgg ttgccatatc gtttttgttc gccgtcagga
    10681 tattttctca ggtgacattg gtccctgccg gtgaatatgc aaaaattcat ccgttcctgc
    10741 ggcccgattc caatgtcatc atccacccgg aagccctgga acagtttttt atcaaactgg
    10801 cctgccttga acacgacacc ctcacccggc tgaggatcct tcatatcggg gactcgcata
    10861 tccaggccga ttttttcagc ggacccgtcc ggaaaatcct gcagcagcag ttcggcaatg
    10921 ccggccgcgg cctcattttt ccttataaaa tcgccaatac caacgggcct tccgatatcg
    10981 atgtcttcag caatacttcg tggaccccca aacgaaacgt tttccctaaa atcgatatgc
    11041 cggtcgggat aagcggcatc agcctgaaaa ccggaaatcc tttcgccgtg atgcgcgtaa
    11101 gccttaaagc ggtcgactcc gtcgcacagc cctacgacca gatgaccgta tatgcccatt
    11161 gcagccgccg ctcctttgac ctggccgttt cattgaatgc cagctcgggc gtcctcgaga
    11221 aatcagcggt ctattcagag aatacctatt acacggtaaa aagcggtgat aacctgggcc
    11281 tgatcgcccg gaaatttggc acaaccgtct ctcagctcaa agcctggaac ggtttgcatt
    11341 ccgactttat ccgggctggt cagaaactga tcatcaaaaa agagcgtcaa gcggtacagg
    11401 cgatgagcct tgaatcgttt acaatgcttg atacattgtt cttctccgac acattgcagg
    11461 gccagcgggg cagggtcatc cggtttgatc agccggagaa aacctgttac ctccatatgg
    11521 tgaaaactga ttccatacag gaagagtttg ttctgcaggg tatccttctc gagaacgcgg
    11581 atcagccggg gatcctctac agcatgtcgg gcgtaaacgg cgccatgtat gagcattata
    11641 acctctcttc cgactttttg cagcaagtga gggattacaa acccgacctg gtcatcctgt
    11701 cgcttgggac aaatgaaacc ctgggcagct atttcgacga ggaaaagact caaatcgaaa
    11761 tgcgcaggat gatcgactcg ctccggaacc tggaaccggc gcctgatatc ctgctgacgg
    11821 ccccgtccga taatatgctc cgccgtcgct acccgaacaa atcggtcatc cggatcaatg
    11881 aattaatggc aaaggaagcc gacagttgcc acgtggctct gtggaacctg ttttcggtca
    11941 tgggcggaca cggcagtatc gacaaatggt acaaggccgg gctggcccgc gccgaccgca
    12001 tccatttcac cgttgccggg tatgaactgc aggccgctct gcttatggat gccctgatgg
    12061 aagcgtatca caaattcaag gacaatggac tggaataaga tcatcggggt ttttcgctac
    12121 gatccggcct cgccgatgtt gttcaacagc gggatattcc tggtcatgtt cctgggattt
    12181 tatggtattt atgttctcat tcgcgaccgc cggactttcc tctttattta taccgtacta
    12241 ttctctctct atttctatta caaagccagc ggattctatg tcgccatctt tatcgcctcc
    12301 accataatca acttctggct gggcaaccgt atctttctga ccggaaataa aaccggcaaa
    12361 aaagcctggc tggtatccgg gatcatcctg aacctggccc ttttgttcta tttcaaatac
    12421 accaacctgt ttctccgatc gtggggcacg attagcggaa gcgaagtcac tcacttcgat
    12481 attttccttc ccatcgggat ctcctttttc acctttcagt cgatcagcta cctggtcgat
    12541 atctacaaaa aattgcttcg gcccccggag aacctgctga acttctcctt ttatgccacg
    12601 tttttcccgc accttgtcgc cggccccatc gtcagggcct atgtgttcct tccccagatc
    12661 taccagaaac tgaaactgac cgcccgacag atcggacagg cctggtggct cattattgcc
    12721 ggactcatca agaaagcggt gatttccgat tatatcagca tcaattttgt cgaccgggtt
    12781 ttcgacaacc cggccctgta ttccggcttc gagaacctga tggctgttta cggctatgcc
    12841 atccagattt attgcgactt ttccggctat tccgatatcg ccatcgggtt agccctgctg
    12901 atgggattcc gccttcccat caacttcaac agcccctaca aggccacatc ggtgaccgat
    12961 ttctggcgcc ggtggcacat tacgctctca acatggctcc gggattacct gtatattccg
    13021 atgggaggga acaggaaagg gaagctcagg acctatggca acctgctgat gaccatgctg
    13081 attggcggtt tgtggcacgg cgcttcctgg aagttcgtag tatggggcgg gttgcatggc
    13141 acggcgctgg cagtggaaaa gctctttcag ccactgattg gcgacaggaa atcggcctgg
    13201 ctaaaggttg ccggctggat cctgaccttt cacttcgttt gcctgggatg gatcttcttc
    13261 agggcggccg actactcgat tgccctggcc gtcattaaag ccattgccgg attttcaggc
    13321 tgggacattg ccctggagat catcacggcc gaatggccgg tgttcctggt cctggcttta
    13381 ggctatgccg ggcatttttt ctcccggcgg tacaaattaa aactggagga gatcttctca
    13441 aagtcaccgt tgccggtgca ggccctggct gtttcgctcg tgatctggat catcctgcag
    13501 gtcgccagcg cccaggtgca gcccttcatc tattttcaat tctgaaaaac cggctaagat
    13561 cagtgtttat tcgtctaatt cgtgtcatcc gcgttctaaa ggccctttcc cccaaccggg
    13621 atcgcataat ccttgaaata tttcatgtac atcaggccat tgggaacgta tttcggatcg
    13681 taggttttgg gtgggcctga ttcagggtaa ggacgtcccg tgttatggat gtggaaggcg
    13741 tctttgtatt gtcctcgccg gatataatcg ccgtaggtca tgtcgatcca tttcactccc
    13801 cagtagaggg ccttgtcgcc tctgagctca ctgagcggaa tatccagcca gatgcacttg
    13861 taacccatga gctggaaggg cccccacgat ttggccatgt ttttcagcgc gtcatcggag
    13921 gcgttgttca ggtcggccgg tacgatattt tccagccggg caagcttgcc gtgttttaca
    13981 ttttgaagcc ttttatagat atgccgttcg aaccggggtt tcacgttctt caggccagag
    14041 cattcgagga tgataagcgc tttgagaaat tcgggtgaaa gatcgaactg ggccgccagt
    14101 ttatcgatct ccgcgccgta gtttttctgg gtacgaagaa ctgcctccgg ggcagtttca
    14161 tcgtaagtga cgctctcctc ggccgtcata taccggtaat accgtttcac agagtcggaa
    14221 aggttcacat acagcatcgg attccagatc catattgcca tcagcaggat gatgaaagca
    14281 gcaaagccca ggctgatctt gctgatcctg cttttcttcc tgccggagga acggctcttt
    14341 ttctttgctg gtttccggtt tttaaccggc ttcttactgg ttttcgccat gcgggctcgt
    14401 tggggttatc ggttcaccaa taacgcgttt attggttgaa attacatatt aatgaggtga
    14461 tagcaggctt ttatcagaac tacttatcga caaatatagg ataatagtag attgtgacca
    14521 acttcaggca tacttagaat ctttctattt tttacccgaa atgcatggat attaatcagt
    14581 tttcaatatt tttacgggga cttttggaaa aaaatgacta ttaatcggat aaagcattct
    14641 ttatcaggaa gataatttat attttaattg aggtttgctg aaagctgata atgaattaat
    14701 tttacctgtt tcaaaaaacc cagaaccaac cgttatgaaa agatttgtca gttatattct
    14761 attgttctta ctattttctg gttatatagc aaccgctcag ggcgaggata ttattattga
    14821 tgaaactaac ctttcgggaa cgatcgggcc cggcagttat attaccgatc ataacctctt
    14881 cgtcgattat acggactgtc tgaccgtttt acccggaacc accattaaat ttatttccaa
    14941 tacttcttta acggtttacg gatgtctgat ttccattgga actgaatctt ccggaattat
    15001 ttacaaatct tcggcaactt ccgtaaaatg gaatgggatc agtttccccg gcagtaaagc
    15061 tttggcatct gatcctgatg atatcctgca atccaaattt aaatattcac attttgagaa
    15121 tgtaactggc ccgggagcca tctatttttc gaacggaaat gctgaaagaa cgctcgattc
    15181 catcagctac tgctctttta ccggcgtggt taacgctatt cacatcctgt ttatcaatca
    15241 ggccggcttt gttatcaaca attgcgattt ctctgatgtt tccaataccg gtataacttt
    15301 tggcaacagc cctgcagttt caggcctgca aattctgaac aacaccttca gcgatctgtc
    15361 cggccggttt ctttatgtct attcaaattc tgcccttgaa tcactaactg ttaaaggtaa
    15421 taccctggat aacctggttt taaagaaatc agccatcaga attgaaaata accagtatct
    15481 gaatgcactg accctcgaat caaacgatta cagcaatttt ataacagacg tcacctaccc
    15541 ggtcgctgat gggataattt acattaaagg ccagaatatt ctcaaccagt tccgcctgga
    15601 tgataatacc atccaaacaa tcctgtcttc tcagcaatta aatccgccgg taagttcctt
    15661 cctttatctt gatcatgccg ccaacgctta ttttaaattg gacaatgtgg tttccgtttc
    15721 cagtttgaat gattttatcc tggtggaaga ttgcgaaact gtaagagtag aagattcaaa
    15781 attcgaatca ttagagtgtt ccggcgccct ttttaacctt tctgccgaca agtattgccg
    15841 tgtggaagat ggtattttca aagacatttc aaccaatgac ggaaatggct gtttctattt
    15901 cagccaggtt gcatcaatgg atgaaatcct tttcagaaat aaccgttttg aagctgtcac
    15961 agccgaaaat ggcccgtgcc tttatgtcaa ttcgccctcc gggtcggtcg attccgttgt
    16021 tttcaggaac aatgacatct tccaggaccg gatgggatat acggccatca atggcggagt
    16081 ggcctatctc cggttgtcat ccgtttcatt gttcaggagc tatgataatg aaatccggaa
    16141 ttgtgaagtc accggatacg gcggtgccta ttgttttgat gttggcacag tcaataatct
    16201 tgatatttat aatgatgatc tccatacgat tatctcgaat tatgacggcg gttacatttc
    16261 ggttaagaat cagataacca ataccgacat aagcattaac ggcctgactg ccgatacctg
    16321 tttttcaaaa gcaggatcag gcggtatcct ttatctgaag tctcaaaaca ataataccgg
    16381 aaaagtcagg atacaaaaca accatctcaa tcatttctat gcccgcagtg aatcgcagca
    16441 atttgccggg ggtggttttt ttatccgggg cagccacaag gagctgcatt ttaccggtaa
    16501 cgcccttatt cccaatccga caccggatca gatcccaatt gcagatgatg gcggagtgct
    16561 ttactatgcc cacactggca gtgttctcga ttcggccatt ttttccggta attcctttat
    16621 taaagccgat tctgtagcca ttacaggttc cggtggagcc gtttacctga caagtacggg
    16681 ccagaccggc cggataagga tcagtgacaa cgagttcgac cggttcagca ctgccgggtc
    16741 aggaggtgtt ttctggatcg gctttgaggg ccagtgtaac cggctggata ttctcaataa
    16801 tgaattctcg ggttgcgttg ccgatagttc cggcggcgcc atctttttcc ggtcatccgg
    16861 attaaatgtc ctaaacgtgg caggccagcc cggtgaaaac cagcttttta ccgactgcag
    16921 ggcccgtttt gcatccggcg gaagccttta cgttttaacc caggcccaat ccatcgggga
    16981 gatcaacttt aaccataatt cattcaattc ccccggctcc gctcaaaatg ccctgtcaga
    17041 cggcggtgct gtttatgtga aatcggccgg ttcactcgtg tctcatttaa acctgtcgga
    17101 aaactctgtc aatgaaaata atatggatat ccgggccggt ggaaacggag gagcattcta
    17161 tttcaatctg atgggtaact gtactgaggt caacctgttg aataattccc tttcttcctg
    17221 ccttgcaggc cgttccggcg gggcagttta tctcaggacc gggaatatcg gcagattgac
    17281 agtggagggc gaaacagcca gcccacaggt gaaagactgt cacgccgcaa acggtggggc
    17341 ttattatttc gatctgtcag gcaaatgcga taccctcgac atcacttcta acaatttcat
    17401 gaactgtgca gccgacacga ccggaggcgc tatttacttt aaatcaaacg ggatcggttt
    17461 gttgaatata tccgcccagg acggagatca gcaaaaattc aataactgcc aggctgccag
    17521 cggatatggc ggtgtgatgt attttaacag cctttcgcag ggcatcgggg aaatcggcat
    17581 tcgtaataac cagatcctgt catcgcttaa cgaaaataac agcggaaaag acggaggcgg
    17641 tttttatttc aagtcggtgg gcagtgatgt ggcaaatatc gatatccgga acaacaccgt
    17701 tggaaacagc aacaaaggat tgaaagcagg catgaacgga ggcgcatttt attttgacct
    17761 ctccgggaag tgcaaccagc tcagcattca tcagaataat ttcaacaatt gtcgcgctgt
    17821 gaacggatcg ggtggcagca tctttttcct ctccggcggg gcaggaactg tttcgattga
    17881 caccaacatg ctgctatcat catccgcaat cgagaacgct tcagccaatg gcggcggaat
    17941 ttacattaaa accaacggcg gaaatgtgac acaaatctca ctgaatggta atgaagcggg
    18001 tgacgacagt tttggcatca gctccggtga gaatggagga gcttttagct ttgatctgca
    18061 gggaactacg acattgctgg cagtgaccgg aaaccggttt gtcaaatgcc gggcgcagga
    18121 ctcgggcggc ggcctctatt ttaaagccaa cgggctgaat gaattaaaac tacagggatt
    18181 cgaagatgaa ttgcagcatt tcagcggttg ttcggctgcc gatggctccg gaggtgccgt
    18241 ttgtatcaca actacatcgc aaccggttag ttatgcctat atcgaataca atacggttct
    18301 tccggcagcc ggcatggtga atgccggtga aaacggcgga ggaatcagca ttacatcagc
    18361 cgggaacctt gtcgaagaat gttttgttga ccacaacata ttcggaagtg cttcaggtag
    18421 tctgaaagcc ggaaatagcg gcggggcaat atcagttgat atttccggtc aatgcaatac
    18481 tctcagatta tttgaaaatt cattcaccgg ctgccaggct gtaaataatg gcggtgcagt
    18541 gagcatccat gccgggagcc tcgggaccct cactgtttca ggctccccgg atcagtacca
    18601 ggtattcggt gattgttcga cagaaaacct tggtggctgc ttctacctag agtccgacgg
    18661 tgtaggtcat gcagatttcc aacaccttaa atttgagaac tcaaccgcag aaagcggagg
    18721 ggccttggct ttttcatccc ctgatatgga tacgctgagc ctcaacacca tcgaatttac
    18781 tgacaactca gcctcagact tcggcggatc gatatattta aaaggagacg ggaacaacat
    18841 gcccaccatt gatgtattgg aaatgaataa atgcaccttt atcaattcgg aagcggataa
    18901 cggggatggc ggcgctttgt ttttcaaagg taacctgggc ctttttaatt gttcatccaa
    18961 cagttttaac caatgtgagg ccaatggggc taacagtggt tcaggttctg ccatgtacct
    19021 cgaactatct gacgatttca cgaatgaatt ggtctttaca tccgatacgg tcaaaaattg
    19081 ccattctgtt tccggaggaa ttttccttaa aaatatcgac aaagccacct tcctggaagg
    19141 ccatctcgtg ggaaataccg ccacccagga cggagcagcc atctatatcc aggatttcga
    19201 taccgttctg attaccggag ggctcttcag ccagaatgaa agccagcaga ccggcggggc
    19261 tatcctgctg aaggccggga attcggaaag ttcctgctgt gatataggtt cctcggaatt
    19321 tgaattcaat aaagccttga acgggggtgc agttaccttg cttgatttta accaaagttc
    19381 attgctgaac aaccgtttca tccagaattc cgctttcagt caatccatgg aatgctctgg
    19441 aggagctgtt tatacagttg atattgatga tcttgttttc gatggaaatg agtttataag
    19501 caattcagcc agcaccactt ccacgcagaa caatattcaa tcatacggag gcagtctgtg
    19561 tatcctgaac aataagtatt ttgagggatc cggaaatgat ttccggtttt caaatgcaga
    19621 ggtgggtgga gctgtcttcg caaagaatac tgatgctaca ctgaacccgt cggtgaagta
    19681 tgacaataat ttcttctata ataatggaag tacttttggc ggggccgttt attacgacct
    19741 tatgaacaaa ggaggcatct atgcaactga aaacgtattc ctgatgaatg gcgcgggatt
    19801 cagcggcggt gcgatcgatc tgagaaacat tgggcggttc gaatccgtgc ggaatatttt
    19861 cagaagcaat ttcaatgagg ggaaaaacct cagcggcgtc gtgggcgctt cggccatctt
    19921 aacctggaac gtgccggaat ccaactatta caatacggtc ttcgattata atttttacga
    19981 aaaagacacg ataaccgaaa gggaatcaac catctggatt aaaaaccacg ataatgtcta
    20041 ttttgagaat tgtacattcc tgaatcaaat accccaggat aatccggtta tttacaagga
    20101 tcagaacagt gaaccgctga ccattgtcaa ttccatcttt tcaaagaatg taactcccga
    20161 aacacctttg gtaaatgact ttgccgagat ccggcactcc tatatcgaag gcctgattac
    20221 agaaaagata gatacgatga attgcttccg ctacccgccg cctcagttcg tcgatggcac
    20281 ttataatctt aatgataacg acttcgcttt tatcgatgcc ggaattcctg aggctgtgta
    20341 taacgattcg attgattacc ccatcggcaa aggaaccgaa acctgcgacc tgggtgtttc
    20401 cggcggccgc tataacctct gggatgagga acaattgtct attcccctgg caaaccagta
    20461 ttcccgtacc atcattatcg aaagatataa cggtgaatgt ggccggtaca gaatttattt
    20521 tgctctgaat gaaggagatg agtttacgga ttatacatgg tatattttcg atgagatcca
    20581 taaaaccgga tctgtaaatg agtttatcgg ttattttcct caggatcaac tcattgctgt
    20641 aaccggggtg gcatcaaatg aaagcgaggg cataacggtt atcggtaata atacgttcag
    20701 tacattctat gaaataacgc tggatgggtc acagatttcc tacgaaggga gcgactacca
    20761 gtgcggagcg accatcgaac tgaatgacaa ttgtccggtg caactgaaca atattgtatt
    20821 gcacctggag ggtctggata tgcctttctt cagttcttat aagtttggat ggtcattccg
    20881 ggatgccatc ggaattgcga gccagaacat aaccgaccag agctatgagg aggttgtatt
    20941 cagcctggat atggaagcgg acacaacttc tctgcgttcc gttaccgttg tgtacaatgg
    21001 ctatgatacc gtatgcgata tcacattcga atatgaatgc ctgatccggt tccattacag
    21061 taacctcgaa aatgtgctgg aactggtgga gattgcaccg cctgatatga tccttgatcc
    21121 tggtaatgtg atgtttgacg taaaattgaa cgacccggct tattactgca acggagttga
    21181 tttttaccta atagaagaag gcagtgttgc aaccagcctg gatttcataa ggctgagatc
    21241 ggtcgcaacc ggaaacacgg tgccgatcca ggggataaca tacgccggca atattttcca
    21301 gttgacattc aatgtaacag accttcccaa aggggaatac gatgtggaaa tgcagaatct
    21361 gtgcaattcc tgcggttttc cgtttacttt agtcaaccgg attgatataa aattcgaagg
    21421 gattgccggg atggctgatt cagagctgaa gatctggccg gtaccggcaa atgagaaaat
    21481 ttttattgaa aatgagaaga cggcatctgt catgcgtttt agcctggtcg acccgctggg
    21541 ggagatcctg tttacggata acatttaccc cggaaagcga tatgaactga ataccggccg
    21601 gtatcccggt ggcatttata ttctgatatt tgagccgttt gattcaaaaa tcaaatcata
    21661 cagaaaaata ataattgaat aaacatatga agaagaaaag tttattgctc attttttcac
    21721 tattcactct cttcggtttc tgccagcagg aggttttctg gtttccggca aaaggcaggg
    21781 tctatggcga tgtgatgcgt atcatggatg ataataatcc ctatgtcagg aacaggattc
    21841 agggttttaa tgaaggagat atcaacgtaa atcctgaata ttggttcaat ggatttaaag
    21901 cgcccgccaa tgattttgaa tggtctaacc agagcggggt ttattattcg caactggatt
    21961 atccatccat tctggcagat aaggatgtca ccacgattat cctggatttt gtaaccgtat
    22021 gggatgagca gttctatgat acggctgaat attattattc aaagccggtc atggagtgtt
    22081 tgaccgtcaa caatggcagt tattcggaaa acatgctgct ggtcaatccg acagaggtga
    22141 cgcttgattt caggttatat gcctcattgt tcaacttgcc cgcaccgacg gatggtgatg
    22201 acaggataga cgggatagtg gaagttatcg atggaaatgt taaaccgatt aatatagccg
    22261 atacccgtaa cgggacttat attcacggtg ttgatctctc ctctttgtcg tacggcttgc
    22321 atgaacttga atattatgct tacggccaga aatcggatgt gaatcttccc gatgagttct
    22381 ctgataaaat caagatcagg atcctgatct ttgattttaa actccgttac gatgaggact
    22441 atgccgtgtg caaatgcgat acggcgtacg acctggaagg aaggccggaa ggggggattt
    22501 tctcgggcga atgcgtagtc ggatcgtcta atgtgtttaa tcccgaactg actcagaatg
    22561 cctccaccct tatcacctat aagtacccgg tggatggaat gtattacaac gtggagcgga
    22621 cagtttcctt tgatccgctt ccggtcattg ccgtcgatgc cacaacgctg gccggttttc
    22681 cgcacgaagc ctgcggcttt gaacacggcg cggtttatga agtgaccggg acggggtttg
    22741 aagatttgtt cagacttccg cctttaggaa taattaaaca gtttcattat gacgggaata
    22801 aattgaccat tgactggaaa ggagagggcg acggctcgat atatgtgaca gccctgagcg
    22861 ataagggttg ttccgccagc ctggaacata tggtgcacat cacattcaac cgggcacctg
    22921 aagacagcgc ttatgttacc ttatatgaca gaatgctttt ctgcaatgcc gacacggcca
    22981 gcgttaaagt attcgactgg tatggtgtga ttgggggaag taatgtgttt tatgaaagga
    23041 ccacgaatcc ctattacgtc ctcgacttta tacccgccgc aggtgactct ttttacgtgc
    23101 tgaccgccga cgatacgacc agttgcagta cccattcgca tatctatacc aaacctgccg
    23161 tccccaaatc ggacctgatc ggggaaggtg cagaacaacc ggctgttaag cttttcccca
    23221 acccggtcag aggggatgta aactgtgaaa tcctgaagcc tgtgaaggaa ggaaggatca
    23281 tcataaccga tgtgcttggc cgccagataa gcaggatcaa ccatgacgga ttccttcccg
    23341 gggaagtggt caggctggaa acaggtgatt ttaaaagcgg gatctattac gtggtatttt
    23401 caggtaataa cctgtcagga tcctataaat tcattgttta ttaaaatcta aaataaccca
    23461 tgatggacaa tcgcgttaaa tatacactat tcatcctgct gacctttgca gcccttaacg
    23521 gattcagcca aaagatcccc gcgaaactgg aggccgaggt aagcgatcag gtctcagaaa
    23581 ctattgcaaa gtttacaagg cagatcagta gcatttacac ttcggccggc gaaacttctt
    23641 tccggtttga tagcgttgca ctcaatcgga cccgtaccat gttctataaa tataatgacc
    23701 cctctttcaa acatatcagt cccaatatta ttgatccttc aaaagaattc ggactatcat
    23761 catttccaaa agatacaaat attctgcctg aaaattttat tgttcccatc gaccaattcc
    23821 tcgactcact ggcagcctgg tattgtaacg acagttgcat tacaactctc gaactgggtt
    23881 atatggatcc gaaaacagat tggaataatt attatattga tcctcagttg ttatgccttc
    23941 aggtctttta taaattcaat ttgttcggag aaccctatat ccccaggtat aacgaaattg
    24001 tggcaagaca acagataacc aaagatcaga atgcggcatt tgacgattta ttcgaggaag
    24061 atgccggatt tgccgggcaa cttgatacaa gaactacggt tatcttcctt tttttattag
    24121 acaattacaa aataaaggaa acatatatct ataaaattta cgatagtttt attgacaaat
    24181 cggtttttat tgaagatgtc cgtcaaatta catctgaaag aaataaatac tacctttcga
    24241 tcaggcctta cataggaacc agtgtcctga aagccgattt ttcaaacatc aataattttg
    24301 agcaaacaga ggagtttcag ccctatctcg gggttcatat cgatttcaac tattatttta
    24361 acgatgggcc aagaagattt acaaaaggac tgagtatggg tgcgggctgg tcgaggttta
    24421 acgcgaccgg taaggttttt gattacaatg cctcatttac ggaattttcc gaaccttttg
    24481 accagaacta tactaaatat gtatatgccg ataatatcca tgaaaacctg atgctggatt
    24541 atgcggatgt caatctgctc attaaactcc ggtaccggtt gttcaagaat acggaactga
    24601 tcggctcaat agggcctcag gcttctttcc tcatcaatag taaaatcgaa cctgaagcag
    24661 gcagcgaagg atttgtcaat tatgaaggat attttaccga tatcgaattt cccaatggga
    24721 ccagcggaga attcctcctt cagaatgtcc ctgaatatgg tttcacaaaa tatactgacc
    24781 tgaagttcag tgagaatgaa attggaataa acaaattcaa tttctgtgcc tatgctgata
    24841 tcggccttaa cctgaagatg agccataagt tccacctatc tttcggtatc gggtacatgt
    24901 atggctttgg caacctggtt tccgaatcat ccggggagga attcgtcctg tcgaaagggg
    24961 agggagaagt ggacaacctc ttcaagaatt gcacatcttt gaaaatcagt gccccaagcg
    25021 cttttgtttc cgctgcttac cttattaaca ctaaaattaa atagccctgc catatgaaac
    25081 aattcatact atccttattt ttcctggcca cttcggtgat gcttttcgga caggaagagc
    25141 aatgcctttt caggacccag ccgcaggata ccgaaacctg tctgaatggc ctggccgtgt
    25201 tcgacgccgg cgtggatacc ctgttctttg aaaacagctt tttcttcgaa tggcagtaca
    25261 aacccgccgg agctgccgat tggataccgg tacaaaataa cggcacgttc caggttacca
    25321 atacccagct tagttctatg ctgacggtta caataaacgg cgacgcctcc cttgacggag
    25381 ctgatttccg atgctcagtc agcgatcttt actatagtga atcagcatcc atggtgatcc
    25441 attcgcttcc ggtcgtttcg tttacctccc agaattattg tttcggagat attaccggat
    25501 ttacgaatac cagcccggac gccgcctcca tgaaaagctg ggcctgggaa ttctcagacg
    25561 gctccaaata catcagccgc gatatcaatc accgcttcct gctgccgggc gactataaag
    25621 tgaaactgac actgaccaac ccgtcggact gcgaagcggc agacagtgcg gtagtgacaa
    25681 tcctgccact tcccgaacct gtaattaagt atacaaagga cgtcttttgc ggatttgaaa
    25741 gcaacctgac cttctacacc gaggatatct ttgaaagtta tctgtgggag atcaggggag
    25801 caaacaacgt cctggcaagt gaagagaccg agataacgtt cgattgcgac gaaaatttcc
    25861 ctacaggaca gtatgaagcc atactaatgg tgaccgatgc taacggttgt tcggcagatg
    25921 tcaggaagag tttcctggta cttagcagcc aggcgccggt cgatggaacc gttttgcaga
    25981 aagaggacga tagccgcctg ctcgtcctgc tgatcgatca ccaggagaat tccgactttc
    26041 tctggatgaa aatggacccg gtcaccaaaa cggtcctcga acaatacaag acgaaagagc
    26101 catatcatct tttcggtgaa ccgattgata aaaacagttt tgaatacggt gtggaggtca
    26161 tccccccatt ttccgactgt agcgcagtat tctatctaaa ttgattttgc gccgttacaa
    26221 tgatatctta agcgtacatt aaagaaatta aatatgatat tgatgaaaaa ggcgaatatg
    26281 aaatcctgga ataaatggtt ctcaatttgg tgctttgcaa tgttcttcgg atcatctgga
    26341 atattgaacg cccaaaatgt tcctttcgag gtaaggaatt tcctggctga ttatgagaga
    26401 gttgttgaca tcagtgaatt tgatgattcc gacacaatgc ctaccaatag gttttcagct
    26461 gcagcagcag aaaaattcac taattttttt ctatctaagg aaacattgat ttttaatgac
    26521 attgctcagg atgaagacat gtcactttat atcactcctg ctgaatacat taagaaagta
    26581 agtgagttat atcccatagg attatatgtt tatatttctg atgctgaaat tattgatgtc
    26641 agggaatatt catcttacat cctctacatc gtggaaattg aaaaaaccat atatatcgac
    26701 aacatgctgc gtacaaaccg gcctgttcca aagttcaatt ctcccgtcag gcagatatat
    26761 tatattatcg gttatagcaa ttctccgggc ataaagatca taggaattga agcaaaaggt
    26821 acggccgccg acaataaaac ggcttacaac ggactggttc cggataagct ggagtttaat
    26881 gcccatttca atgcctacaa cctgaatttt aaagacatcc gcgaggaata tgcaggcctt
    26941 gacggttcgg gcaatcaaac ccgctttgga ctgctgctgg gctgggattt gggtggtaaa
    27001 aagagtttcc ggtatggatt ggcctcggga ctgctttatg acaaggtcaa tttttctacc
    27061 gatctggaaa gttttgccga tcggccgatt ccagcagttg atatagataa ctacccatac
    27121 cggaaaatca ttaccggaca tgacatcagc cagtccaatt cactggacca gctgactcta
    27181 ccggtattat tcagtatgaa ctgggaattt cctcccaagt ggggaaataa gtacgtaaaa
    27241 agatcattca caggagacct ggcctataag cgcgggatcg gcatcaattt caggatcggg
    27301 ccgcatattc agtacaacct gagtggaaag agtgatcctt acgaaggcac attcagttat
    27361 ggaggaagtt accgtttctt caatcctgtt gtacaagaca gtacaactat tattatcgat
    27421 aacctgccgg aatatggttt tgttaacgat acgactttta cggataatag ttctgtcgcg
    27481 agttacagta agatcaatat tggattctcc acacaactga atgtttccat ccccatctac
    27541 aaatcactcg agttttactt tggcccaagc ctctattaca atttttcggg cctggctgaa
    27601 acaactgaag attttatcct gtcaggccgg atcggggaga tcaatccatt tattactgct
    27661 gctaaaacaa gctctttgtc atatggagtc aatgccgggc tggttctgaa cctccacgcc
    27721 ccgcgtgtgc cctattcatc cattgagtta ccggatatag ccaaggatcg ctacaaagtc
    27781 aaaacggcct atactcttga gaatgataat aggatgaaaa tggctttgaa cgttaatctg
    27841 atcgccggga aaagcatgga aatggaacgt caagcgatca attaccagat acagtcggat
    27901 tgggcgaaaa aaccgacgaa aggaaaattt gccggtttta agacccatat tctaaaatac
    27961 gagtacccga aaatagccgg caaagtcacc tttaaaagcg acctcgtcat cgaaaagcca
    28021 ttcggttatg atgtggtttg caacgatacg atcgcattca atagcgtaaa taccaatccg
    28081 ttggttattc cgctcgataa tctgaacagg cttgctgaaa accgtcaatc actcgatctg
    28141 accgtgaacc ggctgcctga tttcacattt gtctatgttt cactttacaa caaatttgaa
    28201 acattggaac aaagaaggaa aatcgtagaa ctggtgcggg atctgggtaa taatgccatc
    28261 atcaaccagg aagaactgct ggtgtacctg agttctgagg ccggtaagcc gatagcttac
    28321 tgcaacttcg cctcaagaga ggatctgcct gaaaatgttg ttaccgactg gcaggagttt
    28381 ctttataaat taaaagaaga atatggctct cagaacctga tttatgaaga tgatattcgt
    28441 aacattgata atatcctgaa ccctatcctg aatcttgatg aaaggatgaa tgcccagcga
    28501 cgttttgtca atttcaattt tattgttaat gacccggaga agtattatgg ttacgactcg
    28561 gaacgtcttt ctgatacaag ggctttaatc catttccttt ccgataaata ttgttcgaag
    28621 attcctttca ggtcctcaca attcgatttc aatgtctatc ttactgaaag aaatttgaat
    28681 atgagaaaat ctcccagtga tatcaatatt aatattattc atttaatcaa ataatgtttc
    28741 tgcaatgaaa aaatacatat cccaactgct aattttaagt tccttcttct taagcctgca
    28801 aatggtatcc gggcaggaac tgatcagaaa accaaattcc gagctgatca acagtgtgaa
    28861 tgagatcatt ggaaagacca gctacgagcg cggaacgaca agatggttcg tttattccaa
    28921 caggaaaggc aatattgtat acagcgatgc gggttcggtg ataagcagca acgtgaagtt
    28981 cctggaaact tttaatgtgg ttgcggaaga taacaacagg ctcaaagtta aaaagctgga
    29041 tggcgaggtc gtgggctgga tggacaagga tgacctgatc atcctggatc attgtgaacg
    29101 caaagccaac atggtttcca agaaagcaat gatcatcaat acgatcgaca gagccagcgc
    29161 taatgctaac agagacagca gccagttccg gttttatttc gaccctgacc tgacaaagag
    29221 aaacccaaaa gaacaaagca gtgtctatga gatcttatat gtttacaagg aaagtggaaa
    29281 tgcattactg ctgggcagac gcgaagactg ttcggtcgac gttgaagctt atctcaaaga
    29341 atcgatcatc ggatggattg ccaaagaatc ggtaaccgag tgggattccc ggggatgcct
    29401 tttgcctaat tataaacctg aagcaaaaac tgaaagaagg acccagggca cacagccggc
    29461 tattctcgat accagttcaa gatatactga ttattcgctg gccaaggctt atgactccgt
    29521 cttattgact tacgcgaacc taaagggtta tgctgaaact tcctttgaca gatcacctat
    29581 tgtatggaat gaagaaattg ataatcgatg gccaccttcg aaattcaggc tgccttacct
    29641 tgacaaacat aataacgata tcgatatcat gaaggttggt gttctgggtg atattaccga
    29701 tgcgcagggt agacagatcc aggcattaat tgataacctg agggaatggg agcgccagaa
    29761 caggacgaaa aacagcctga acctggtctt cgtcatcgat ggtacctata gcatgaagaa
    29821 ttattttcca gccgttgcca acgccatcag cgaaagccat aagatcatca gcaaagatcc
    29881 cgacttctcc gaccctgata acccgaacag ggttaccgtt aagtgcggcg cagttgttta
    29941 ccgcgatctt gccgaaggcg agtaccttgt ggaaaccagt cctcttcagg actttaatgc
    30001 cacaagaaac tggatatcat ccaatttcag cgacatgaaa aacaggaacg accgcgatat
    30061 ccccgaagcc gtattcaacg ggctctatta tgccatcgag gatgtattgg ttggccatga
    30121 agaggagaat aacctgatca ttctcatcgg tgatgccggc gaccggaacg atcctgaaga
    30181 cgaagtatat atcgaaatcg acagcctggt ctatggttta tacaaaaaca attgcaattt
    30241 tatcgcttat caaacccact ggaagaatcc ccaggagaat cccgacaata tcagctatac
    30301 cgagttttgc gaacaaatgt cgaccatcag tgagagatcg gctcaaatga tccagaaaaa
    30361 gatacagggc atccgtaatc tcgaccttga aggttctgtg aggctggttc aagtggaatc
    30421 gacatcatcc tataacttat atgaactcga ccagccattc ctggccaaca aagtatattg
    30481 caacaagggc agccagatcg acgcggcaag gcttaccgag ttcatcattg catctatcaa
    30541 agcatccagt aaaaatgtat cgctcgtcaa caagtttatc cacgaaatcc tttacagcgg
    30601 tatgtcatgg gatgaaatcc tccgaaaata taatatttcc caggacaatg cggccaactt
    30661 ggggccgatt gctacgaccc tcggctatat cgtcggcgaa cttaaaaaga tctatcccag
    30721 ggaggatgtt accgctccgg gggggcgcct ggaacaactc ctccagggta aactgcagtt
    30781 gtattttgaa ggatttaccc ctctcagaat caataattcc aataacaact tatggacttt
    30841 tgagattctg accatcgcgc aaaccaagaa gaatctcgaa agagattata tgcaggtgat
    30901 taacgctttc agaaaccagg gctctgcaga ccagagaaga caattgattt ataatgcatt
    30961 catgacgctg gcccgcacct atgccggtga tgaaactaat tatgaaaacc tgaccattgg
    31021 cgtcttgttt gcaaaaatca tcgattgccc cggattgaag ttctcaagca gatacaggga
    31081 ttgccttagc aaaaccattc gtcagatcag attggaacaa gaatgtccgg atgaatgtct
    31141 tgatgtgttc tatactgaaa tttaccggtc ctataacaga ataaccaatc tttcatataa
    31201 ggattatgta acgactgcat tcggcacttt ttcggcatat tatattcccg ttgagttaat
    31261 gccgtttgta aactaatagc gacgattttt tcttaaacct gttatatcat gaataatagt
    31321 atagaaatta aaaatgcctc tttcgtttat aaaaccaaga tttctaagaa ggagacgatt
    31381 gagagagagg tcttacatgt aagaaatctc gaaattaaac agggagagat cacttttatt
    31441 ataggccgct ccggatcagg gaaaagcaca ctcctggaaa ctcttggaat gatgaacgat
    31501 actttcccgg ttgatacgaa agagatggag ataaattttc atctcaaaaa agatgaagag
    31561 ccacttaata ttttcaaaaa gaagatctgg gaaaaaaaga aagataccga ggcgttgctc
    31621 aatcagataa gactgaacaa tttcagtttc atcttccaga acacaaacct gctgaacaat
    31681 tttaccgcta tcgataatgt gtgcattacc gatatgctta atggaaattc atacagtgaa
    31741 gcctatctta aaagcctgat tatattttcg ctcttaggcc tgggcgactt ggatgtgggt
    31801 attaaacccc agcatatttc cggtggtcag cgtcaaagag tcgcttttgc aagggccatt
    31861 attcctgatt accagttcct tttcggtgac gaacctaccg ggaacctcga caaggaaaca
    31921 tctaccgaag tacttcagct gctttccgat tttatccgcg gcacgaactc ggaaacatct
    31981 accattatcg tttctcatga catcgacctg gcactgaagt tcgccgacag gatcattgtc
    32041 attaccaaag aagacatggg agatattaaa cccgagaatg tcttctacag gggagaagac
    32101 caccagaaga aatcaggacc ggcaaaatct tatatcttca agtcgaaaat ccataaggcc
    32161 tttaaggaca atcccgaact gatcgaagga tttaagatcc tggtccagga tgctgttcaa
    32221 cagctcgaaa aggaatcaaa atcggaattt aaaattaagc ccggtttttt tcaaaagatg
    32281 tactccctgt ttgaagcaaa agaagtcagg ttttccgaga tccggaacat taaagtaaac
    32341 agtcttctgc gtaagatact gttcctgatg cagaacccgg aacaacggtc ggcaggtatc
    32401 agggggctga acttcttcga tgaaacattt tttaatgaac ttgaatcgat cctggaagcc
    32461 aaaggaacta atttagccgc taaaaacggt gaggagtcag aaaaagccga agccaaatca
    32521 tggagaagaa aaactgattc aggagaaaaa gccctgggcg acggtgaact cagaagcatg
    32581 ctgtcggtat tactgaaatt ctttgaaaga gaagataaac tggcagtctc aggatcaaca
    32641 aaatcgattt ataaaaatga aaattcattc ttctttttcc tttttatctc catcagccgg
    32701 ttgctcagcc gcattataac tttgaaaaat gataacctgg ctacccctaa tgccgatatt
    32761 aataatcccg attttgccag gctctttata cggaatgaag gagggatcct gctgggagcc
    32821 ggcaagaaac tgacgcattt caggacgctt ttctttgcac tctttgtgac cttcctgggt
    32881 atcggttttg ccactggcag tttgagctat ctgaaggata aaatggatga cccgtttgtg
    32941 aacttcgtca ccacatccgt cccctctgaa aaacaatccg aggtggcttc cattatccag
    33001 cattataaca gggaatcgga actgaagaat caatataaga tcaatgtttt taccggtttc
    33061 acgattttcc ctgttgagat ttacaataag aaaaacaatg acctctcttt gatcacgggt
    33121 cggacgatca cactgaacga tccgctgttg aaagagttat ttaaggataa ggataatatt
    33181 attacaggga atgagacggg attattcaat gattatgatt gcgggtttat tgtgagcgaa
    33241 tccctgataa aaaaactggg aatggagccc gatgcacggt tcctaaaggt caagatcctg
    33301 acaacgaata cctccgctcc cgatacggct tcaaccggtg agattgcttt taatgaagtc
    33361 acggataatt acgaactggt gccggttccc atccgggcca tactcaagaa gatcccgggc
    33421 gatttgaacc aggatatcga ctttatcatc accgagtatg cacacctgaa cctgcttgac
    33481 aatcatgagc cttcaaaaga gtatcccttc catcccagcc ataca
//
LOCUS       DAOPNH010000005        32468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_1152_length_32468_cov_6.489032, whole genome shotgun sequence.
ACCESSION   DAOPNH010000005 DAOPNH010000000
VERSION     DAOPNH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1152_length_32468_cov_6.489032"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKZ74_00765"
     CDS             complement(<1..204)
                     /locus_tag="PKZ74_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91308.1"
                     /translation="MKKASFRVFAVLLVTGAIAVLVANKPPVDDSYVIISWNDLGMHC
                     ANKDFANICVLPPYNNLKAQVILK"
     gene            complement(225..1151)
                     /locus_tag="PKZ74_00770"
     CDS             complement(225..1151)
                     /locus_tag="PKZ74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91309.1"
                     /translation="MKTKSTFTLITAIWITILMIAGTRAQSQENIIVQIGESSMSKGL
                     QTCYSVRIPQAEIKAVQQGWIKHLQEDNKAKVKEIGHELHFSGGLKTEIFPDTISLYS
                     LLIATDSAINLNVFLEIDSVFFTPNSDKTDLASDKIDNSLRNYIRNFAVDQYRIAVED
                     ELKAAEKVLKDLQNDLEKLQKDEKNLLNDNVKMENEIESTELELTQFDNSLAIKNQEI
                     IDHNARMQNIALETEKEAAKAKQKELEKEKNQLEKSRGKAKELISDNKSQIEKNKKAI
                     KQSQEDQDLKQQDITKQNNLIMVVQNKLKGIK"
     gene            1298..2185
                     /locus_tag="PKZ74_00775"
     CDS             1298..2185
                     /locus_tag="PKZ74_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91310.1"
                     /translation="MKALFHKKFLEHNIDSDSEGAYRLLSFMDTYEAPEVLGEPYINL
                     VHSERYIEYIREACENGDFVAEVALTPASYEAACLAVGMTIRASEQGDFAVVRPPGHH
                     AGREKAAGFCFFNNIAIAVQKLVNEGKKVFIFDIDGHHGDGTQSIFYDSDRVLYCSIH
                     QHYAYPYTGFPDETGDGAGKGFNFNFPLISGSGDKDFLIAVDKAIDVARKFNPDVVAV
                     SAGFDAYYKDKLLGLQVTQRAFYECAFRTRRAFSNIFAVLEGGYHNDVRECIDNFIEG
                     IHNGAKPPVLKWDTDMSIG"
     gene            2188..3087
                     /locus_tag="PKZ74_00780"
     CDS             2188..3087
                     /locus_tag="PKZ74_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM91311.1"
                     /translation="MNLSEHYGELAALATAMFWTITALSFEFASKRVGSLAVNMIRLT
                     FAFILLGIFTLITRGMFLPLDATSFNWMWLALSGLIGLVLGDYFLFKSYTVISSRVSM
                     LIMTTVPPMTALIGWLLLGEKMIPMHILGMFLTVGGIFLAIFSRPVDGGRIRLSYPAK
                     GILFALVGAAGQAGGLVLSKYGMAGYNSFAASHIRLIAGMAGYAMLILFLGKGGLIRK
                     AIKDGKGMRAIGLGSFFGPFLGISFSLLAVKYTETGIAATIMAIVPILIIPPSVWLFK
                     EKVTWMEIAGAVISVGGVVLMFL"
     gene            3425..5671
                     /locus_tag="PKZ74_00785"
     CDS             3425..5671
                     /locus_tag="PKZ74_00785"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPM91312.1"
                     /translation="MKINIKYLRISPLIFIFIILTSIPVITNAQDGLKTKTKVRPTVG
                     LVLSGGGAKGFAYIGLLKVIHEAGLPLDYIGGTSIGSIMGGLYALGYHPDTIDTMIRS
                     IDWDALLADKIERKYISFEEKEFGEKFIITLPVKSKGVILSPSLYQGQEIELLLNRYF
                     SPAFSIKDFDRLPTPFLCMGTDLFTGAEVVLDSGYLPKAIRASMSIPGYFSPVDYGSY
                     FLGDGGVVNNYPVKEVKERGIDIMIGGDVQPPLRTTREEFNSLTDVIDQIITFNRGSA
                     NQIGRELTDIYVPLNTRYGIMDFAEYDSLIAFGERVARPFFSRFKALADSLNQIEYRA
                     PLTFNTRPVSEVFINSVQIKGYKKFAGSYFRNYFNLKEDTVFTLDEIERSIRQIYGTR
                     FFEKVEYEVNTDGHRSDLVIRVREADPGYLSAGLHYDNDYQASLLITGSFRNVLGKRT
                     KLFADLVLGQNPRLRAFYMLDNGAQPGIGARFETYSLKFDIYEREKKINRIVFSNYKT
                     SAFINYSLKNLFNFRAGFDYEYFRFRQDVAADTALEAFSDFSSYGSFFAEFSADSRDR
                     AYFTRSGSKSELRLEYVMPLSKNWSRDLFTSSFILYWKYDVSEEVAPKLVLRPGIFLG
                     STMSSENRPPLQHNFGFGGLNPNQYVNTILPFTGLQFIQKTGYHAIILRMRLQYNFYK
                     KLYATALFDGGAAEEIFDDVFEPANFIAGYGAALGYDSFIGPLEITFMGSNRNSRPMV
                     FLNLGFWF"
     gene            complement(5703..6515)
                     /locus_tag="PKZ74_00790"
     CDS             complement(5703..6515)
                     /locus_tag="PKZ74_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPM91313.1"
                     /translation="MNNKKILVPVDFTRLSDKVIEHAATIGIRAGSDLVLLHCADRSA
                     DLDTLKIKLAEKAVKASRKFNITCDSKVRTGNIFTAIPEEADDSSYYLTIIGTHGVKG
                     LRQKLLGADILRLVTKIPGPTLVVQSSSDIAAAYEKIILPVATHQSYENILKSVIILA
                     KIFHSEVQVYSIERPGFVAPEQLKKNLQRTVEIFDSEGIKHQRINEKQTVLSVGFATQ
                     TLQFAVKSGAQMLAVMSVPSEEYHYFAQQDKENLLTNREGIPVLCASNQGNE"
     gene            6784..9582
                     /locus_tag="PKZ74_00795"
     CDS             6784..9582
                     /locus_tag="PKZ74_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPM91314.1"
                     /translation="MKNNKYIPRQIVLIFIILLPYWSVLSTFSQSNEDCLMCHEDPTL
                     TAIRQGKTVSMFVNEKILISSVHDGMECTSCHPDAGVEEFPHPEVLPPVDCGFCHDKA
                     FAEFDRGIHGQALRLNALYAPTCKECHGSHDILSRTNPKSRTYKMNIPVLCGKCHREG
                     APVARTYNISEHNILENYSESIHGEGLFKRGLIVTATCNDCHGNHLVLPHTSPNSTIS
                     INNIANTCMQCHAEIERVHTKIIKEELWEKEPGAIPACTDCHPPHKVNPQNIVATISD
                     KSCLECHNSDAVHKIADGQRVSLKVVPEDITFSVHKNINCVKCHSDVSPTLARPCETA
                     NRVDCSNCHAEVSNVYAASGHGQAYGRREANAPYCTDCHGTHRVKSRYDDTSPTYRTA
                     IPALCGDCHRSDGKASQVEGLKEINAYADYSTSVHGRGLTEKGLLPSAVCTDCHTSHF
                     ILKDSDERSSVFPKNIPATCATCHKGIYDEYIKSDHAITVHDEKLQYPTCADCHSAHV
                     ITDISQDVFVNEVTNQCGKCHKDLADTYFETYHGKAYLLGSEKAAKCSDCHGSHSIFM
                     ASNPMSSVGSRNIVETCKQCHPDANQRFTGYLTHATHHNKAKYPALYYTFWGMTTLLV
                     VVFAFFGIHLLLWLPRSFQAMKKKRMKQAETGEELHIRRFSRSQRITHLFVILSFILL
                     ALTGMTLKFAALPWAAFLADLMGGVHVAGNIHRFAAVITFGYFIYHVYYLIKSKIRNK
                     TKWGSFIFGKNSLMFNKQDLKDFWATFKWFLGLGPKPGYGRWTYWEKFDYFAVFWGVA
                     VIGFSGLILWFPEFFTTIFPGWLINVAMIIHSDEALLAVGFIFTIHFFNTHLRPDAFP
                     MDKVIFTGTVPMEDYKHERPREFEELEQSGRLEEVLVKPEQNKEIDRIIRVFGFTMLA
                     IGLTLVVLIIYSMLFGYN"
     gene            complement(9682..11754)
                     /locus_tag="PKZ74_00800"
     CDS             complement(9682..11754)
                     /locus_tag="PKZ74_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM91315.1"
                     /translation="MKKTLMAALLLTGLIMNSFSQTDTVYYDGFDDKRTGWAKDTEEN
                     SYKVENGNYVWTRKQAGEWNSWWTIPGINAEEFSIEARFRIDGDGSFGFLWGSKDLEN
                     SHYFLVNNAGMARSYIESRNTTSVIKENIPALGFQPGFNILTLSKKDNRIDVLINGNR
                     IHEEFYSGFFGNLFGFSFQGISTYSIDYFLIKHKINLVPGIKYTGKPENLGFNINTRS
                     SEICPIVTPDGQGLFFIRVEDPENTGGTSDYSDIYYAKTGADKKWQQAVNLKKPLNNS
                     STNAVNSVTPDGSTLFLLHTYNDDGTFKAGGLSLSNKTATSWEIPEDITVIDHYNLND
                     YNEFCMSNDKKVIIMAVEREDTYGEKDLYVSFNIEGNLWSVPLNLGPVLNSAQSEISP
                     FLAADNTTLYFSSDGHPGHGDNDIFLARRLDETWMNWSKPENLGKPINSSDWDAYYSV
                     PASGEYAYFVSSKSGFGNSDIFRIKLPEMARPNPVVLVKGKVLNQKTKTPVQAVINYF
                     DLQNSAISGTAMSNPETGYYQITLPAGRLYGFLASSEKFISVNENVDLKDLDEYQELE
                     RDLYLVPIEIGEKVRLNNIFFETGKWDLLPESFAELDKLVRILTDNPNMEIEINGHTD
                     NVGNDESNLMLSQKRAAAVVNYILDKGITSTRLDSAGFGEAQPVATNDTDEGRRLNRR
                     VEFMIVKM"
     gene            complement(11907..14144)
                     /gene="bglX"
                     /locus_tag="PKZ74_00805"
     CDS             complement(11907..14144)
                     /gene="bglX"
                     /locus_tag="PKZ74_00805"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HPM91316.1"
                     /translation="MRTYLFQLLTIAAFLYSINISAQHKTIDQKVDSVLSLMTLGEKI
                     GQLNQYNDDWGATGPVTLDTDKENQIRNGKVGSLLNCTGTVRTRLWQDIAMQSRLRIP
                     LLFGQDVVHGYKTTFPIPLAESCSWDPDAIEMAARVAAEEASACGIHWTFAPMVDIAR
                     DPRWGRVMEGAGEDPYLGSVIAFARVKGFQGNNPDGNGSLMACTKHYAAYGAAIGGRD
                     YNSVDMSERLLREVYLPPFKATVDAGVGTFMTAFNDINGVPATGNSLILRDILKGEWG
                     FEGFVVSDWGSVGEMIQHGNVKDEADAARCAITAGCDMDMESRCYIKTLEQQVNEGMV
                     PVSLIDAAAKRILKKKFELGLFADPYKNCDPERERMALENPSHTAAARDMARKSIVLL
                     KNENDLLPLSKDLKTIAFIGPLVKAVKQNKGFWDVEVPGVDSNFIVTQWEGVEAKVGA
                     KTKLLYARGCDIDGDSKDGFAEAVAVARQAEVVILSIGERRDLSGEARSRSDIGIPGV
                     QEELLKELLATGKPVVVLINAGRPLVFNYTADNAPAILYTWWLGSEAGNAIADVLFGD
                     YNPSGKLTMTFPLSVGQVPIYYSHLNTGRPATDDVDRFYRSAYIDLSIYPRYEFGYGL
                     SYTGFEYSNLMIDRKMIKTDEKITVSVTIKNTGRYDGEEIVQLYLRDRVASISRPVKE
                     LKDFTKIFLKAGESKTVTFIIDKEKLSFYNNDLEWVAEPGEFDLMIGASSRDIRLTEN
                     FVLTE"
     gene            14334..15569
                     /locus_tag="PKZ74_00810"
     CDS             14334..15569
                     /locus_tag="PKZ74_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91317.1"
                     /translation="MKPYPLKPGRGTYPAERIVGREREITQLLRLLITQSVSVEELRR
                     MGKTLLVKKLEYICNRNLVPEEFKQEGFKVKYLSFEGIQTLGGLIDTLIREFEKIKDW
                     SKIDWARTYEFIRKIIKSQKVKIAEAEFSINLPEYKRTWQEAFFKVLEDIADAQETNG
                     SKLILIFDELPLMLWEWHKKGNAGEGIELLDILRKKRQELEEKGLRFVYCGSIGMNII
                     LKTYKKLYGYAGPATNDMEPFSVDPFTEEDAFFLCDCFEAGGFKTSNKEQCYPLIYQL
                     SNGLPFYISQMFNIIQIDFDKEVNEETVKSAYRIILNNPDYHNDFNQLKDRINIYYPE
                     NEAKVMLYILNFLSQKETIINQEEILSGLAIEDRIIKDQLYQMTKDHLLSRTQSESGT
                     WQYQFKHEIFRQWWQINQA"
     gene            15545..17185
                     /locus_tag="PKZ74_00815"
     CDS             15545..17185
                     /locus_tag="PKZ74_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HPM91318.1"
                     /translation="MVANQSGINTLLSFGVKSVNPEILEKLLIGRQKAADYLYDAAKS
                     ISEDGINQHILVIGQRGMGKTHLLRVLFHRIQPFIKEKKVVVAYFSEEEYGIANYFDF
                     LVRILNSFLKWYGEEQDNIKNKIAELQNTPFSSQISKAEEIIEEFCSGKPLLILAENF
                     SDILAALSPGNEQGQLRSWLYTSDRISIIATSQALSNDFDKENRPFFGFFTNYYLKNL
                     TFPESLEFLIGISILDNRADVTDFLKHKGRPQIKALYELVKGNHRLLVTFYEFLKTDT
                     LARLSTHFIKTINDLKPYYESFIRYLPPQQQKILRYIALSRKPQNGVDICKNCFIEQR
                     TFSKQISELTRKGLIEAITDPTDKRNKLYDINEPLLRISIEVGEHREGITALFVDFLA
                     NFYSREELLERKSKFISQQQSCANEPEARQLYDESNDLDIAIIEKDIDILMQRFDKIN
                     DGYDLNPREKIFNQINDSIIQILKISHCKKQVNFESLKAIAIQYQLQFPVFKITEKYI
                     DIFQNYVVEGDKKAIYSLPKEQREFFKKEILGEKKEGI"
     gene            complement(17287..17460)
                     /locus_tag="PKZ74_00820"
     CDS             complement(17287..17460)
                     /locus_tag="PKZ74_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91319.1"
                     /translation="MRSQDNPFINQLQVLVISTRSHQHRIVKIRGIDPLLDRRVIIRD
                     VDDLARGFPENTG"
     gene            17548..20136
                     /locus_tag="PKZ74_00825"
     CDS             17548..20136
                     /locus_tag="PKZ74_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91320.1"
                     /translation="MGANARICGFSITNGLGDEGGGIKISSEYPIPEYRVIATLDHLI
                     VSNNEADKGGGIYIYWCDPILDDCRVYGNSAYGLGGGIYTENSSVSMNALCISSNSSG
                     YKGGGIYINGSSPALNNLTIAGNSTGAMGGGIYCSSGYSMPVLCNCNITGNNATSGAG
                     IGNDGNLSVNQCHVSGNIATWEGGGIYTDDSIIIINLVIDSNTAEYGGGIWINGDASI
                     DTTEITGNHANAYGGGLYLTNSSGSNLNKVLVSENHSESYGGGFYAENSADINLKAST
                     ISYNRSEILGGGIYQLNDQQISFDNINRCNIFLNRAPIGNDLYLYGDKLDVIVDTFTV
                     LHPTDHHARYLENFTFDILSGKVQQVEADLFVSPEGDDDNSGLTPEEPLKTINRAYAK
                     LITDKEHLHTVYLADGIYSQTTTGEIFPVHIINYSSLAGESCPGTILDAEGSYHVISL
                     SDDTVPSIRNMTISGAMNIYSGGGISCYTSQVCMNNLRVTGNFSGDTGGGIEIRYGSK
                     VTMSDMIIDHDSAQYGGGLYIDNGTDTVLMSNVQILDNYAAVQGGGIYIYFTKGFSLI
                     NSSLEGNSSKAGGGAYMDRTSPLIKNTLFANNSANYYAGGLEIYYQFSEDIENSILTN
                     CTFADNENLGLLCYGMGAKLTNCVFRGNTGAQVMLVGLWNNDTLFFDHSNVSGGLDSI
                     IVNGSAKLEWLEGNIAEDPHFVLDGDYPYALADDSPCIDAGTADTTGLFLPLNDLAGC
                     VRVWDGDNDGTTVIDMGAYEYGAPVGITDKNPDLDKKDGALRLYPNPATDKVTVSFSD
                     DRSGHRVIFMNSSGQVVMTEIISSGISKTDISVKPFSTGIYFVKAYSETGGIAMGKIV
                     VLRANW"
     gene            complement(20271..21635)
                     /locus_tag="PKZ74_00830"
     CDS             complement(20271..21635)
                     /locus_tag="PKZ74_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91321.1"
                     /translation="MKRLTVFWILITIYSFCPAQITFDKTYDFWEGMKLTDVFPVEDG
                     YFLTGHIERTGGFYFLIKTDLYGDTIFTKKYYPEKHLKDEDPFMLTRNDAEIYLAYEI
                     NNGIYIQKFNLNGDSLWSRKVTDFGGLTEIIWTADSNFLILASNANLHLYKCDLFGNK
                     IWESYCEMVDSNLIMSGIASSIIEMPDGKIKASVYYSALESHFGQTIYSFSDSGTFLQ
                     KNEIASPYDAASIYEMMNYEEDIIAIGNNMIDRFYASRLSPENDTLWVRHYLFNIFGG
                     YFDDITRDINNNYIMAGMIGEAHTGNFDLGLICFSADGDSLWWHNYHYEGYKSIAYDI
                     ELCPDGGLAVVGIIEDLPDVKGKFLKTNELGLISGPGISEIKPRNAVKIYPNPASGVL
                     YIEATEPVESIDMFDNLGCKVLRVYGCKGEKVEVPLYGLAPGLYLVRVDTDEGVVARK
                     VVVK"
     gene            complement(21746..25189)
                     /locus_tag="PKZ74_00835"
     CDS             complement(21746..25189)
                     /locus_tag="PKZ74_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91322.1"
                     /translation="MKNLSFLLLSLAIFLLFALQGLCQQDFLPPFNFRVDSTTLMATW
                     EAPKIVLLNEDFEGDMFPPEGWADTTLGSGWQCDENPLYHAWWMVPPHTGKVALVNDD
                     WIPNNNGSLDFLYFPEMDLTFADSFSLRFDSYFDGAYGQTAYVKYSVDDGDTWLLLKQ
                     MEPSLLWQRVEIDLSEFSGTNGISNFKLAFYTNDRPDNLGWWGSGWAVDNVAVISGET
                     TNQAFSYKVFLDTTLIDCVPSTYFQYSFEYNTTHICAVMACYNNYNSDPVEQSVHSAC
                     FPKPTELGGSAPDEAVILTWSPPMVMHDGPAISIAREHGDVLRNFPSPPPISSCYGIC
                     DDGTGLWVSDPNRSGITIFKVTYDGVNTGETITLSQGQSWIGDMVSDGNYLYCCLIGG
                     SNRIGKVYLPTGEIVGTIGGDFTVEPQMGLAADFENEEFYIGGWNSNMIWRTHFNGNT
                     ISTHNINNVSGLAWSPPGSNFPVGTLWVTTYASTSWIHRIDPNSNWIQFESFMIPGGE
                     PYAGAGIEIKTSGYFQWTSLWICNQVENRIYLADIEEILIPGPQPSVPDNLLGFNLYK
                     NGEFLDYIDYTAPDSCGYVDPISSSEYYEGSVLKYEVNAVYDMTPYGFPGETEESLPE
                     GPAEIILFIYWFELDFLEDWSAGNFDDNAWYITDSNWTVSQDIGNEAPCAVFMPAAPI
                     TDYEATLESYHFLELSAHELILEYDVSLSSVNPTGNEKLQVQVFDGTNRSWNTVKTFT
                     NAEGSFDWRKDSLDIAGAFNTGRLRIRFNATGVNSSDISYWAVDNIALTRICPAPDSI
                     ETTLIPSSEDSVQVTWAEPTPPIAEWRQWDDGVPANGIGFGTSKDSWFNFAIRWTPEL
                     LIELKGASITAVGFIPYDVSACFKIALWSGDDPVPFYTQAAGNLNALQWGVFQLDTPQ
                     KIDITKDLYVGYQISTYCDYPISVDNGPAVDSLGNLFRFGNGDQWTTLLEINPEFNWN
                     LNIKAFFERDGIQYESFYRVYRGIDGSEPELITETIENELIDPVGSGTGMYCYKVQTL
                     FYNGCESEFSPESCVLITKIPFIEEVETGTVKIYPNPASEVLYIESSEKIESVRIFDC
                     RGITIEQLSNRTVEQTNGRTGEQARDHVLKIPLNGLAPGLYLVRVETGGGVVARKVVV
                     K"
     gene            complement(25332..27137)
                     /locus_tag="PKZ74_00840"
     CDS             complement(25332..27137)
                     /locus_tag="PKZ74_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91323.1"
                     /translation="MKTPKTFFFIVWLLFAINGVVAQNGSFIQKQLPCQVSHEPRFTV
                     TATVQDDDTWCKVEWPDPVGYYPELWYDDGEADDYFLFMNPGGMNALKFITYFDNFIV
                     TGGRIFVGDGSFPGPFLGTTFRVLVYDDDGSDGFPGTVIDSTDIMVENYGWVEFEGLT
                     AQFAESNFYLAMKQLAPSPDAAPIGVDTDNPTYFRSYSNFSGTWVLCPLQDLMIRATV
                     MAYNVPVREVDSFEVARFSGFEMNEPPLQGDTLILDTILPSEYNDYAWDSLDDGLFAY
                     GVRTHFTNGQWSDYDVSNIVIHRYYLYMPSCFYQSDDMMLTACLPLDSNGNIPFNLLG
                     LMLYRDMDFVEYISCAPEPPADTIEIAISDDTPPGIYTFQLTAVYDLSSYGFPGETGE
                     SGYLATEAVVSNCNPPTELEANYDQGLGAMALGWSAPIGVFQDQFDTFAVYRSDNGSP
                     YYLRAYTGQLFFLDDYAICENENDYHRYRISALYTNGTDTCESEFSNEDGDLCLGIEH
                     RFNNSFLSIYPNPASEVLYIESPEKIERVSIFDCRGITIEQSSNRTGEQTNGRTGEQA
                     RDHVLKIPLNGLAPGLYLVRVETGGGVVARKVVVK"
     gene            complement(27275..28450)
                     /locus_tag="PKZ74_00845"
     CDS             complement(27275..28450)
                     /locus_tag="PKZ74_00845"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HPM91324.1"
                     /translation="MKKIDDRLVTAAEKHVSVLLTDQLPETVLFHNLSYVQKLDKQAE
                     TIGKHEGLTEEEMNILRIGALFLYSGYTGGGDKVREKSAEIAAEFLVKEGIEPEAVDL
                     ISRIILSAGAEAKPEGLIAEALCDADLMFLASENAIEQFDLLFDECVMLRPGQEKRPV
                     YEKRWVDFLTSHQYYTNYGKTKLQDAKEETLRRLSDRMNRRRLIEARKESASEKRFSY
                     SRGVDTMFRITARNQINLNSIADNKSNILISVNAIIISIIITMLAGNIGNMSNDIIPI
                     LVLLVVCMVTITIAILSTRPNIVTSKFSGADIQNHQVDLNFFGNFIRMDYDEYLNAVR
                     DMMRDDEYLYSVLLKNQYELGKILSKKFRLVRIAYNVFMIGMIVTVVAFIVNYLFVH"
     gene            28875..29924
                     /locus_tag="PKZ74_00850"
     CDS             28875..29924
                     /locus_tag="PKZ74_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91325.1"
                     /translation="MMRITPAIILFLLFLTGAESFSQDKNAGKTVPLFESDDIIHLDL
                     RADFKAVFAATDDSTYFPAELIWQSDDGKNAAFDVEIRQRGETRRKKTTCSFAPLRLK
                     FPKNITDGTPFEDQKTIKLVTHCNKGDAFEQNIIAEYLVYKAFNVLTDSSFKVRPALI
                     RYISTGKKADTLQKFAFFIEREKHLAQRLKGDEIETEKVHPDRLDPFQTCLVDLFEYM
                     IGNTDYSIYERHNMVIVSNKTGQPPFISVPYDFDWSGLVSASYAVPNPKFNTSDVKVR
                     IYRGLKRDRETVDRAIRLFNERKQEIYRVFESYELLEQNERKQIARYLDEFYWTINNE
                     AQVRVEFFERARERE"
     gene            29982..31607
                     /locus_tag="PKZ74_00855"
     CDS             29982..31607
                     /locus_tag="PKZ74_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM91326.1"
                     /translation="MDNGQCTMDNVRLILFLLISGLLFPYVVFGMKDAEKTAVKAIRA
                     DDTTGLRIYMASHPGPNCEFSNGKTGLYYAIVYDAINSAEFLLAGGAEPDDTVDGNAL
                     LKWAIKYNRPRITRFLIEFGAGINTPDDFGNTPLMYAASVNNLEICRLLVNRGADPMI
                     KNPYGRRASDFATAESETPLFRYLLFMERMIMEQDSLPDMLDGPYIFQENDRRLTMKY
                     YERSEEMNRTFISEKTLTPVIQDLVVSGFGWDTNTYRITGSFDPDSCIFETTGDVFVI
                     GDIHGRYQALVNLLINNKIIDDELNWRFGNGHLVLLGDVFDRGDKVTETLWLLYGLQF
                     QAKESGGNVHLLLGNHEVMAMTGDHRYLHDKYQFFMRYTSSYYDELYDETSLLGRWLR
                     SRNVILQINGNLFLHAGISPELASTGLTYSQINSSVRDYLSNPKKVKKGSPEELIMGE
                     NGPLWFRGYMNAEGYPSVPYRFVGEYLQVMGLNRMIVGHNEQQYINTSFEGKVIFADV
                     AIDATGKSSQGLLISGDEFYRCYSDGRRESMPL"
     gene            31712..>32468
                     /locus_tag="PKZ74_00860"
     CDS             31712..>32468
                     /locus_tag="PKZ74_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91327.1"
                     /translation="MQTSCFTESQRQAKLPENLKFLLALVLLTLSFEGFSQVAINSTG
                     LPPDPDAMLDVSAINQGVLIPRILILDRPPAKPGMLMYQIDGIPGYEPGFYYADGHTW
                     NRVGHSANDYWLPAVGPDIRFPYRVALGGFTDAEDHGLNVQNYLSGKAAVRGAEMDPN
                     FGTLYTEGMLGVLEPSLLGVPFNVHDAGVLGIARSSYLSSRGAAVYGWNNMSDAAETY
                     AGIFISDGSSFTNYGVYSQASVGITNYGLEARAT"
BASE COUNT         8568 a         7548 c         7625 g         8727 t
ORIGIN      
        1 tttaaggatt acctgggcct ttaaattgtt atatggcggc agaacacaga tatttgcaaa
       61 atccttattt gcacaatgca tgcccaggtc attccaggag atgataacgt aggaatcatc
      121 aaccggtggt ttatttgcaa ccagaacagc aatagctcct gtaacaagca ggactgcaaa
      181 aactctgaaa gaagcctttt tcataggtag tttttttaat taatctattt aattccttta
      241 agtttatttt gaacaaccat gatcaaatta ttttgcttgg tgatgtcttg ctgctttaag
      301 tcctggtctt cctgactttg ctttatcgct tttttatttt tctctatctg tgatttgtta
      361 tcggagatca gttctttcgc tttgcccctg cttttttcca actggttctt ctctttctcc
      421 agttcttttt gtttggcctt ggccgcctct ttttcggttt caagagcgat attctgcatc
      481 cgggcattat ggtcgataat ttcctggttc ttgatggcca ggctgttatc gaattgcgtt
      541 aactcgagtt ctgtcgattc aatctcattt tccattttga cattatcatt caaaaggttc
      601 ttttcatcct tttgtaattt ttccagatca ttctgcaaat ctttcaatac tttctccgcc
      661 gctttgagct catcctccac cgcaatcctg tattgatcga cggcaaagtt ccggatataa
      721 tttctaaggc tgttgtcgat cttgtcgctg gccaggtctg tcttatcgga atttggtgtg
      781 aaaaaaacgc tgtcgatttc caggaacaca ttcaggttta tagcgctgtc ggtagcgatc
      841 aacaggctgt aaaggcttat cgtatccgga aaaatttctg ttttgagccc gcctgagaaa
      901 tggagttcgt ggccgatctc ttttactttg gctttgttgt cttcctgtaa atgtttgatc
      961 cagccttgct ggactgcttt tatttccgcc tggggaattc tgaccgaata acaggtttgt
     1021 aacccttttg acatggagga ctctccaatc tgtacaatga tgttttcctg ggactgtgcc
     1081 cgcgtgccag caatcatgag tattgtgatc caaatggcag taatgagggt aaaggttgat
     1141 ttcgttttca taatcagttt atttaaaagg ataaatgtac tgcatttgga tcggttttcc
     1201 aagaattgag cccgataaat gcccatactg tttaaaaaca attgaattat tgtaatgaat
     1261 tagtaaattt gttcgatcta gatctggtta aaggagcatg aaagcattat ttcacaagaa
     1321 attcctcgaa cataacatcg acagcgacag tgaaggagcc taccgtttgc taagctttat
     1381 ggatacctat gaggcgccgg aggtgctggg ggagccgtat atcaacctgg tccattccga
     1441 acgatatatt gaatacatca gggaagcctg tgaaaatggg gattttgtgg ctgaagtcgc
     1501 acttactcct gccagttatg aggcggcttg cctggctgtt gggatgacga tcagggcttc
     1561 ggagcagggt gattttgccg ttgtgcgccc tccgggacat catgccggac gtgaaaaagc
     1621 ggccggtttc tgctttttca acaatatcgc gattgcggtc cagaaactgg tcaatgaggg
     1681 taagaaggtt tttattttcg atatcgacgg gcatcatggc gacggaacac agtctatttt
     1741 ctatgattcc gaccgcgttc tttattgttc tattcaccag cattatgcat atccatatac
     1801 tggctttccc gatgaaacgg gagatggggc cggtaaggga tttaatttca attttcccct
     1861 gatttcggga agcggagata aagattttct gattgccgtc gataaagcca tcgatgtggc
     1921 gcggaagttc aaccctgatg tggtcgcagt ttccgccgga ttcgatgctt attacaagga
     1981 taaactcctg ggtttgcagg taacacaaag ggccttttac gaatgcgctt tccgcacccg
     2041 ccgggccttt tcgaatatct tcgccgtact cgaaggcggt tatcataacg atgtaaggga
     2101 atgtatcgat aatttcatcg agggtatcca caatggcgcc aaaccgcctg tactgaaatg
     2161 ggataccgat atgtcgatcg ggtaggaatg aatttgtctg agcattacgg tgaactggcc
     2221 gcactggcga cggcaatgtt ctggaccata accgcccttt ctttcgaatt tgccagcaaa
     2281 cgcgtcggat cgctcgccgt gaacatgatc cggttgacat ttgcgtttat cctgctcggc
     2341 atctttaccc tgatcacccg cggcatgttc ctcccgctcg acgcaacatc gttcaactgg
     2401 atgtggctgg cgctgtccgg tctcatcggg cttgtcctgg gcgactactt cctgtttaaa
     2461 tcttatacgg tcatttcctc ccgcgtctcc atgctgatca tgaccaccgt tccgccgatg
     2521 acggccctga tcggatggct gctattaggg gaaaagatga tcccgatgca catcctgggc
     2581 atgttcctta cggtcggtgg cattttcctg gccatatttt cccgtccagt cgatggcggc
     2641 cgcatccgcc tttcctaccc ggccaaaggg attttgttcg ccctggtcgg agctgccggg
     2701 caagccggag gacttgtgct gagcaaatac ggtatggccg gctataactc atttgctgcc
     2761 tcccacatcc ggctgatcgc cggcatggca gggtatgcca tgctcatcct gttccttgga
     2821 aagggaggac tgatcagaaa ggcaattaaa gacgggaaag ggatgcgagc catcggactt
     2881 ggctcgttct tcggcccgtt cctgggaatc tccttctccc tattggctgt caaatacact
     2941 gaaacgggga ttgccgcgac gatcatggct atcgtcccca tcctgatcat tcccccgtct
     3001 gtctggcttt tcaaggaaaa ggtgacctgg atggagatcg ccggtgcggt gatcagcgtc
     3061 ggtggcgtgg tgctgatgtt tttgtaaaaa ggcggatttc gaatttcaga tgcgaaatgc
     3121 tttcagatcc ttcgctgcgc taccaatgac aagttagtaa atttctactg aactgctctc
     3181 ctccggagta atgaaaagct aatgtcatcc aatattgtgg agtttgtaaa agtctgaaaa
     3241 ctcaggatga cggttgccgc gccgaccatt cgtttgcgcg gttctgccgt catcctgagc
     3301 gcagcgaagg aactgccaat ttgtggaaat tccttcccca atccagtatc gagtttcgat
     3361 tatcttaaaa actgtccggc aaatttcaat atctttacct gccgatcatt cttggccaaa
     3421 catcatgaaa atcaatataa aatatcttag aatttctcct ctcattttca tatttattat
     3481 cttaacatct attccggtta tcactaatgc ccaggacggg ctgaaaacaa aaaccaaagt
     3541 tcgccccacc gtcggtctgg ttctcagtgg aggaggagca aagggctttg cttacatcgg
     3601 gctgttaaag gtcatccacg aagcgggcct gccactggat tacatcgggg ggaccagcat
     3661 cggaagcatc atgggcgggc tctacgccct gggataccac ccggatacga tcgataccat
     3721 gatacgctcc atcgactggg acgcgctgct ggccgataag atcgaacgaa aatatatctc
     3781 ttttgaagaa aaagagttcg gggaaaaatt cattatcacc cttccggtaa aaagtaaagg
     3841 cgttatcctg agcccgtcac tttaccaggg ccaggagatc gagcttttac tgaaccgcta
     3901 tttttctccg gcattctcca tcaaggattt tgaccggctg ccaacgcctt tcctctgcat
     3961 gggcaccgat ctgtttaccg gagctgaagt cgtcctggat tcgggttatc tgcccaaagc
     4021 catccgggcg agcatgtcga tcccgggcta tttttccccc gtcgattatg gcagttactt
     4081 cctcggcgat ggaggggtgg tgaataatta cccggtcaaa gaggtaaaag agaggggaat
     4141 tgatattatg atcggcggcg atgttcagcc tcctctccgg acaacccgtg aagagttcaa
     4201 ttcgctgacc gacgtgatcg accagatcat cacctttaac cggggcagcg caaaccagat
     4261 aggacgggag cttaccgata tctatgtgcc gctgaacacc cggtacggga tcatggattt
     4321 tgccgagtac gactcgctga tcgctttcgg agagagggtt gcgcgcccgt ttttcagccg
     4381 cttcaaagcg ctggccgatt ccctgaacca aattgagtat cgcgctccgt tgacttttaa
     4441 tacccggccg gtcagcgaag tttttatcaa ttcggtgcag atcaaaggct ataaaaagtt
     4501 tgccggatcc tacttcagga actatttcaa tctgaaggag gatacggttt tcacgctgga
     4561 tgagatcgaa aggagcatac ggcagatata cggcacacgt tttttcgaaa aggttgaata
     4621 cgaggtgaac acagatggtc accgcagcga tcttgtgata cgtgtcaggg aagccgatcc
     4681 gggatacctc tcggccgggc tgcactatga taacgactac caggccagcc tgcttatcac
     4741 aggctccttc cggaatgtgc tgggcaaacg gacaaagctg tttgccgacc tggtcctggg
     4801 acagaatccc cggctgcgtg cattctatat gctggataac ggtgcccagc cgggaatagg
     4861 cgccaggttc gagacttatt ctttaaaatt tgatatttat gaaagggaga aaaagatcaa
     4921 ccggatcgtt ttctccaatt ataagacatc agcctttatc aattacagcc tgaaaaacct
     4981 gttcaatttc cgggccggat ttgactacga atatttccgc ttcaggcagg atgtcgcggc
     5041 cgataccgcc ctggaagcct tcagtgattt cagcagctac gggtcgttct ttgccgaatt
     5101 cagcgccgat tcccgcgaca gggcctattt tacccgcagc ggttcgaaat cggaactgag
     5161 attggagtat gtcatgccgc tgtcgaaaaa ctggagccgg gacctgttca ccagttcctt
     5221 tatcctttac tggaagtacg atgtgagtga agaggttgcg ccaaaactgg tcctgcgacc
     5281 gggaatcttc ctgggcagta ccatgtcgtc ggagaacagg cctccgctgc agcataattt
     5341 tggttttggc gggttgaatc ctaaccagta cgtcaatacg atccttccgt tcaccggtct
     5401 ccagttcatc cagaaaaccg ggtatcatgc tataattctg aggatgagac tgcaatataa
     5461 tttttataag aagctgtatg ccaccgcgtt attcgacggc ggtgccgccg aagagatttt
     5521 cgatgatgtt ttcgaacccg ccaacttcat tgccggttat ggcgcggccc ttggatacga
     5581 cagttttatc ggcccgctgg agatcacttt catgggatcg aacaggaaca gcagaccgat
     5641 ggtgttcctg aatctggggt tttggttttg agagggttgg aaaggttgta atacaaccct
     5701 tatcactcgt tcccctggtt gcttgcacaa agcacaggaa tcccttcccg gtttgtcaga
     5761 agattttctt tgtcctgctg ggcaaagtaa tggtattcct ctgacggcac cgacataaca
     5821 gctaacattt gagcgcctga tttgactgca aattgtagag tctgtgtggc aaaacccacc
     5881 gaaagcacgg tctgcttttc gttgatccgc tggtgtttta ttccttctga atcaaagatc
     5941 tcaaccgttc gttgaaggtt cttcttcaat tgttcggggg ccacgaagcc gggcctttcg
     6001 atggaatata cctgcacttc cgaatggaat atttttgcca ggatgatcac tgatttgagg
     6061 atgttctcat acgactggtg agtggcaacc gggaggatga ttttctcata tgccgccgct
     6121 atatctgaag atgattgtac gaccagcgtc ggaccgggaa ttttagtaac caggcgtaga
     6181 atatccgctc ccaggagctt ctgcctgagg cctttaacac catgagtgcc tatgatggta
     6241 aggtaatagc tgctgtcgtc ggcctcttcc ggtatagcgg tgaaaatatt acctgtcctg
     6301 actttgctat cgcaggtgat attgaatttc ctggatgcct ttaccgcctt ttcagccagc
     6361 ttgatcttca acgtgtcaag atcggcggaa cggtcagcgc aatgaagcag gacaaggtcg
     6421 gaaccggccc ttataccaat ggttgctgcg tgttcaatga ctttgtcgga taagcgcgta
     6481 aaatcgacgg ggaccagtat ctttttgtta ttcatcgtga agattattat tcaatcagaa
     6541 ggtgtgaatg gttgaatctg taaaagtagt tcattccttt gcttatcaag ccaaaacaca
     6601 catatccaat ttgttaaata cctgttaaat aatgaatgaa tatttgaatg gactattttc
     6661 gttctggtat aaaaagaaat tgagagatta agggtaatcc aacttagaat ctttataaat
     6721 tacatcctga cgtataacat tcgtatatat ttgaatattt tcgtatttat aaattttcaa
     6781 tccgtgaaaa acaacaaata tatacccagg cagatagtgt tgatattcat cattttactg
     6841 ccttactggt ctgtcttgtc cactttctcc cagagcaatg aagattgcct gatgtgtcac
     6901 gaagacccga cccttacggc tatccggcag gggaaaacgg tgtcgatgtt cgtcaatgaa
     6961 aagatcctca tcagcagcgt ccacgacggg atggaatgta cttcctgtca tcccgatgcc
     7021 ggcgtggagg agtttcccca tcccgaagta cttcctccgg tcgattgcgg tttctgccac
     7081 gacaaggcct ttgccgagtt cgaccgtggg attcacgggc aggcattgcg gctgaatgca
     7141 ctttatgcac ctacctgcaa ggagtgccat ggaagccacg atattctttc ccgcaccaac
     7201 ccgaaatccc gcacctataa gatgaatatc ccggtgctgt gcggcaaatg ccaccgcgaa
     7261 ggggcgccgg ttgcccggac ctataacatc agcgaacata acattctcga gaattactcg
     7321 gagagtattc atggtgaagg ccttttcaaa agaggcctga tcgttacggc cacctgcaac
     7381 gattgccacg gaaatcatct cgtgctgccg cataccagtc ccaactcaac catctctata
     7441 aacaatattg caaatacctg tatgcaatgc cacgcggaga tcgaaagggt acacactaaa
     7501 attatcaagg aagagctatg ggaaaaggaa ccgggcgcta ttccggcatg taccgactgc
     7561 cacccgccgc acaaggtgaa tccccagaat atcgtcgcca ctatctccga taaatcctgc
     7621 ctggaatgcc acaactccga cgctgtgcat aaaattgccg acggccagag ggtatcgtta
     7681 aaggtagtgc ccgaagatat aactttctca gtacacaaga atatcaattg cgtgaaatgt
     7741 cattcggacg tttctccgac actggcccgc ccctgtgaaa cagccaaccg ggttgattgc
     7801 tccaactgcc atgcggaggt gtcgaacgtg tatgccgcca gcggccacgg gcaggcctac
     7861 ggccggagag aagccaatgc accctactgc accgactgtc acgggaccca ccgcgtgaaa
     7921 tcgagatatg acgatacttc gcccacctac cgaaccgcta tccctgccct ttgcggcgac
     7981 tgccacaggt ccgacgggaa agcatcgcag gtcgaagggc tcaaagagat caacgcctat
     8041 gccgattact ccaccagcgt ccatggaagg ggtctgaccg aaaaaggcct gttgccaagt
     8101 gccgtgtgca ccgattgcca tacctcccat tttatcttaa aggattcgga tgaacgatcg
     8161 tcggtatttc cgaaaaatat accggctacg tgcgctacct gtcacaaagg catttatgat
     8221 gaatatatca agagcgacca tgctattact gttcatgatg aaaaactgca gtatccgacc
     8281 tgcgccgatt gtcattcggc ccatgtgatc accgatattt cacaggatgt ctttgtgaac
     8341 gaagtgacca accaatgcgg aaaatgccat aaggaccttg cagatactta tttcgagaca
     8401 tatcacggca aagcatacct gctgggctct gaaaaagcgg ccaaatgttc cgactgtcat
     8461 ggctcccaca gtatatttat ggccagcaat ccgatgtctt ccgtcggcag ccgtaatatc
     8521 gtcgagacct gcaaacagtg ccatccggat gccaaccagc ggtttacggg ctacctgacc
     8581 catgctaccc atcacaataa agcaaaatat cccgctttgt attatacatt ctggggaatg
     8641 accaccctgc ttgtcgtggt atttgccttc ttcgggatcc acctgttact ctggctgccc
     8701 agatcgttcc aggcgatgaa gaagaaaaga atgaagcagg cggaaacggg cgaagagctg
     8761 catatccgac ggttctcccg gagccagcgg atcacccacc tgttcgttat tctcagcttt
     8821 atcttgcttg cattgaccgg aatgacactg aaattcgccg ccttgccctg ggccgctttc
     8881 ctggccgatc tgatgggcgg agtccatgtc gccggaaata tccatcgctt tgccgccgtc
     8941 attactttcg gctattttat ctatcatgtt tattatctga tcaaatccaa aatcagaaac
     9001 aaaacgaaat gggggagctt tatcttcggg aagaactccc ttatgttcaa taaacaggac
     9061 ctgaaagatt tctgggccac attcaaatgg ttcctgggat tagggcccaa gccgggttac
     9121 ggcagatgga cctactggga gaagttcgac tactttgccg tattctgggg agtggccgtc
     9181 atcggatttt caggcctgat cctctggttc cccgaatttt tcacgactat cttcccggga
     9241 tggctgatca atgttgccat gatcattcac agcgatgagg ccttactggc tgtcggattc
     9301 atcttcacga tccatttctt caacacccat cttcgtccgg acgcattccc gatggataag
     9361 gtcatcttta ccggaaccgt tcccatggag gattataagc atgaaaggcc gagggagttt
     9421 gaagaactgg aacaatcagg aaggctggag gaggtactgg tgaagcccga acaaaacaaa
     9481 gagatcgaca ggatcatacg ggtctttggc tttaccatgc tggccatcgg cctcacactg
     9541 gtggtgctca tcatctattc gatgctgttc gggtacaatt aggttccaga gttccaggtt
     9601 ccaggttcca aggttccagg ttccaggttc caaggttccc ataatttcca atgacgcgag
     9661 cgcagcgagc tgatgaccaa cctacatctt cacgatcata aactccaccc tgcggttcag
     9721 tcggcggcct tcatcggtat cgttcgtggc aaccggttgc gcttcgccaa aaccggctga
     9781 atcaaggcgc gtgcttgtaa tgcccttgtc caggatgtaa ttcaccacag cggctgccct
     9841 tttctgcgac agcatcaggt tggattcatc gtttccgaca ttatcggtat gcccgttgat
     9901 ctcgatctcc atgtttggat tgtcggtcag tatcctgacc agcttgtcga gttcggcgaa
     9961 tgattcgggg aggagatccc atttgccggt ctcaaaaaag atgttgttca gccttacttt
    10021 ttcgccgatc tcgatcggga ccaggtagag gtcgcgctcc agttcctggt attcatccag
    10081 gtctttcagg tcaacatttt cgttaaccga gatgaatttt tcgctcgaag ccaggaagcc
    10141 gtaaagcctc cctgccggca gcgtgatctg gtaatagcct gtttcgggat tgctcatggc
    10201 ggtgccggat atggccgaat tctggagatc gaaatagttg atgacggcct gcaccggtgt
    10261 tttcgtcttc tggttaagca cctttccttt gaccagcacc accgggttgg gacgggccat
    10321 ctcgggaagc ttgatccgga aaatatcgct gttgccaaag cccgatttgc tggatacaaa
    10381 ataggcatat tcgccggagg ccggcacgct gtagtaggcg tcccaatcgc tcgaattgat
    10441 cggcttgccg agattttccg gtttgctcca gttcatccag gtctcatcaa gacggcgggc
    10501 caggaagatg tcgttgtcgc cgtgcccggg atgaccgtcg gagctgaagt agagcgtcgt
    10561 attatccgcg gccaggaaag gcgatatttc gctctgcgcg gaattgagca ccggccccag
    10621 gttaagcggc accgaccaga ggttgccttc gatattgaat gaaacgtaca ggtctttttc
    10681 gccgtaagta tcttccctct caacagccat gatgatcacc tttttatcgt tcgacatgca
    10741 gaactcattg taatcattca ggttataatg gtcgataacc gtaatatctt ccgggatctc
    10801 ccacgaagtg gcggttttgt tggagaggct gagcccgccg gctttgaagg tcccatcatc
    10861 attatatgta tgcaaaagaa acagggtact gccgtcgggc gtcaccgaat tgaccgcatt
    10921 ggtcgaactg ttgttcagcg gtttcttcag gttaacggcc tgctgccatt ttttatccgc
    10981 tcccgttttt gcataataga tatcggaata atcggatgtt ccgcccgtat tttcgggatc
    11041 ttcgacacgg atgaagaaaa gtccctgccc gtcgggggtt acgatcggac aaatttcgga
    11101 cgaccgggtg ttgatattga aaccgagatt ttcgggttta ccggtgtatt tgatcccggg
    11161 tacgagattg atcttgtgtt tgatgagaaa ataatcgata gaataggtgg aaatcccctg
    11221 gaaagagaac ccgaacaggt taccgaaaaa cccggagtaa aattcttcgt ggatcctgtt
    11281 tccattgatc agtacatcga tccggttatc tttctttgaa agtgtaagaa tattgaaacc
    11341 gggttggaaa cccaatgccg ggatgttttc ctttatgacc gacgtagtgt tccggctttc
    11401 gatataggat ctggccatac ctgcattgtt caccaggaaa tagtgcgagt tttcgaggtc
    11461 ttttgatccc cacaggaatc cgaagctgcc atcgccatcg atccggaacc gggcctctat
    11521 cgaaaattct tccgcattga tcccgggaat agtccaccag gagttccatt cgccggcctg
    11581 tttccgggtc cagacatagt tgccgttttc aactttgtat gaattctctt ccgtgtcttt
    11641 ggcccagccg gtccgtttat catcgaaacc atcgtagtaa acggtgtcgg tctggctgaa
    11701 ggaattcatt atcaatcccg tcagaagcag ggcagccatt aatgtttttt tcatgaaaaa
    11761 ggggttgttt ggttttgaat agcaaaaata atcatcagtt ttatcaatgg ctgttaaagg
    11821 attgttaaag agatgaaaga tttgacagtc cgtcggtgta tgaaaggttt tcatcaacgg
    11881 gtaaatccgt cggtgaaaga aaccttctat tcggttagca caaagttctc tgtcagccgg
    11941 atatcccgag atgaagcgcc gatcatcagg tcgaattcgc ccggttcggc cacccattcc
    12001 aggtcgttgt tgtaaaacga taatttttct ttatcgatga tgaaggtgac ggtcttcgat
    12061 tcgccggctt tcaggaagat cttggtaaag tctttgagtt ctttcacggg acgggaaatt
    12121 gaagccaccc ggtcgcgcag gtagagctgc acgatctctt caccgtcgta ccggcctgtg
    12181 tttttgatag taacggaaac ggtgattttt tcatctgttt tgatcatctt cctgtcaatc
    12241 atcaggttgc tgtattcgaa accggtatag ctcaatccat aaccgaattc gtaacgggga
    12301 taaatggaga ggtcgatgta ggcgctgcgg taaaagcggt ctacatcatc ggtggccggc
    12361 ctgccggtat tcagatggct gtaataaatg ggcacctggc caacgctgag cgggaaggtc
    12421 atggtcagct tgcctgaagg gttgtaatcg ccgaacaaaa cgtcggctat ggcattgccg
    12481 gcttcgctgc cgagccacca ggtgtagagg atggccgggg cgttgtcggc tgtgtagtta
    12541 aataccagcg gccggccggc gttgatcagc accaccaccg gtttgcccgt cgccagcagc
    12601 tccttcaaca gctcttcctg cactccggga attccgatat cacttcggct cctggcctcg
    12661 cccgacaggt ccctccgctc accgatgctc aggattacga cttcggcctg gcgcgctacc
    12721 gcaacggctt cagcaaatcc atctttgctg tcaccgtcga tatcacagcc cctggcatac
    12781 aggagtttcg ttttagcgcc gactttagct tcgacgcctt cccactgcgt cacaatgaaa
    12841 ttggaatcga caccggggac ctcgacatcc cagaaacctt tgttttgctt gactgccttg
    12901 accagcgggc cgataaaggc aatggtcttc aggtctttgg agagggggag gaggtcgttt
    12961 tcatttttga ggaggacgat gcttttccgc gccatatccc tcgctgcggc ggtatgggac
    13021 gggttttcga gcgccatgcg ttccctttcc gggtcgcagt ttttgtaagg atcggcgaaa
    13081 agcccgagct cgaacttctt cttcaggatc ctttttgcgg cggcgtcgat cagggaaacc
    13141 gggaccatgc cttcattgac ctgctgctcc agggttttga tatagcaccg gctctccatg
    13201 tccatgtcgc aaccggcggt aatggcgcag cgggcggcat cggcctcatc ttttacattg
    13261 ccgtgctgga tcatttcacc gaccgagccc cagtcgctca ccacgaatcc ctcaaatccc
    13321 cattcccctt tgagtatatc gcggagaatg aggctgttgc cggtagccgg aacgccgttg
    13381 atatcgttga aggcggtcat aaaagtgccg acgccggcgt cgacggtggc tttgaacggg
    13441 ggcagataga cctcccggag cagccgttcg ctcatatcca ccgagttgta gtctcttccg
    13501 ccgatagcgg cgccataggc ggcatagtgc ttggtacagg ccatcagcga tccgttcccg
    13561 tcggggttgt ttccctggaa gcctttaacg cgggcaaagg cgatcacgga tcccaggtac
    13621 gggtcttcgc ctgccccttc catgacgcgg ccccagcgcg gatcgcgggc gatatcgacc
    13681 atcggggcga aggtccagtg gatcccgcag gcgctggcct cttcagccgc cacgcgggcc
    13741 gccatttcga tcgcatccgg gtcccagctg cacgattcgg ccagcgggat gggaaacgtg
    13801 gtcttatagc cgtgcaccac atcctggccg aacagcagcg ggatccgtag ccgcgactgc
    13861 atggcgatat cctgccacag ccgcgtgcgc accgtgcctg tacaattgag cagcgatcct
    13921 acttttccgt tcctgatctg gttctcttta tcggtatcga gcgtcaccgg gccggtagct
    13981 ccccagtcgt cgttatactg gtttagctgg ccgatctttt cgcccagcgt catcaggctc
    14041 agcaccgaat cgaccttctg gtcgatggtt ttgtgctggg cggagatatt tatggagtaa
    14101 agaaatgctg cgattgtgag cagttggaat aaataagttc tcatattcaa atggataatt
    14161 tctggttttt atcaaaataa aattactaaa tatcatgata aatgaacgga ggacaattaa
    14221 tctgatgatt ttgatagttt aacagcttag tagaataata tacgataaaa gtattgtacg
    14281 ataatattga cgtaagataa aattatcgta tatttgtccc tcaaatttta attatgaaac
    14341 catatccatt aaaacccggg agaggaactt accctgcaga gagaatagtt ggacgtgaaa
    14401 gagaaataac acaactgtta agattactta taacacaaag tgtttccgtg gaagagttaa
    14461 gaaggatggg aaagacactt cttgtaaaaa agttggaata tatatgtaat agaaatctcg
    14521 ttcctgaaga attcaaacag gaaggattca aagtcaaata cttgtctttt gaaggcatac
    14581 aaacactagg aggattaata gataccctga tccgtgaatt cgaaaaaata aaggactggt
    14641 ccaagataga ttgggccagg acatatgagt ttatccggaa gataattaaa agccagaaag
    14701 tcaaaattgc agaggcagaa ttttctataa atcttccgga gtataaaagg acctggcagg
    14761 aagctttctt taaagtcctg gaagatattg ctgatgcaca ggaaacgaac ggcagcaaat
    14821 tgatcctgat tttcgatgag ttgcctttga tgctttggga atggcataaa aaggggaatg
    14881 ccggcgaagg gatcgagtta ctggatattc tcaggaaaaa aaggcaggag ttggaggaaa
    14941 aggggcttcg gtttgtttat tgtggttcta ttgggatgaa tattatcctt aaaacataca
    15001 aaaagctata tggttatgcc ggtcctgcca ctaacgatat ggaacctttc agtgtcgatc
    15061 cttttactga ggaagatgca ttctttttat gtgattgttt cgaggccggc ggatttaaaa
    15121 cttcgaataa agagcagtgc tatccgttga tttatcaatt gtctaatggt cttccttttt
    15181 atatctcaca gatgtttaac attattcaaa ttgattttga taaagaagtc aatgaggaaa
    15241 ccgttaaatc tgcctaccgg atcattttaa ataacccgga ttatcataat gattttaacc
    15301 agttgaaaga caggataaac atttattatc ctgaaaatga agcaaaagtg atgctttata
    15361 tattaaactt tctttcacaa aaagaaacaa ttattaatca agaggagatc ctgtccggtc
    15421 tggcgattga agatcggatt atcaaggacc agctttacca aatgaccaaa gatcacttac
    15481 ttagcaggac gcagagtgaa tcaggtactt ggcaatatca gtttaaacat gaaattttca
    15541 gacaatggtg gcaaatcaat caggcataaa cacgctcctg tccttcgggg tgaagagcgt
    15601 aaacccggaa atccttgaaa aacttctgat cgggaggcaa aaagcggcag attatttata
    15661 tgatgctgcg aaatcaattt ctgaagatgg aatcaaccag catattctgg tcattggaca
    15721 gcgcgggatg ggaaaaaccc atttgttgcg ggttcttttc catcggattc aacctttcat
    15781 taaagagaaa aaggttgtag tcgcctattt ttccgaagag gaatacggca ttgcaaacta
    15841 ttttgatttc ctggtccgga tacttaactc attcttgaaa tggtatggcg aagagcagga
    15901 taatatcaag aataaaattg cagaattgca aaacacacca ttttcttcac aaatatctaa
    15961 agccgaagag attattgagg agttctgttc aggaaagcca ttattgatcc tggcagagaa
    16021 tttttccgat attctcgccg ccttaagccc cggcaatgag cagggacaat tacgctcctg
    16081 gctttatact tctgaccgga tcagcattat agcgaccagc caggctttga gcaacgactt
    16141 tgacaaagag aaccggccct tttttggttt ctttaccaat tattatttaa aaaacctgac
    16201 ctttcccgaa agccttgagt ttctcatcgg gatctccata cttgataaca gggcggatgt
    16261 caccgacttt ttaaagcata aaggcaggcc acagatcaag gcgctgtacg aattggtcaa
    16321 aggcaaccac aggcttctcg tgacctttta cgagtttctg aaaacagata ccctggccag
    16381 gctatcgact cattttatta agacaatcaa tgacctgaaa ccgtattatg aatccttcat
    16441 ccgatatctg ccgccacaac agcaaaaaat cctccgctac attgcgctga gccggaaacc
    16501 acaaaacggg gtggatattt gcaagaattg cttcattgaa caacggacct tcagtaagca
    16561 aatcagtgaa ctcacccgca aagggctgat tgaagccatc accgatccga ccgataaaag
    16621 aaataagctc tatgatatca atgagccctt gttacggatc agtattgaag taggcgagca
    16681 tagggaaggc attaccgcac tattcgtgga ctttctggct aatttttatt cccgggaaga
    16741 acttctggaa agaaaatcaa aatttatttc acaacaacaa tcttgcgcta atgaaccgga
    16801 agcaaggcaa ctttatgatg aaagcaatga tcttgatata gccattatcg agaaggatat
    16861 tgatattttg atgcaaaggt ttgataaaat caatgatggg tatgatttga atcctcgaga
    16921 aaagattttt aatcagatca atgattcaat aattcaaatt ctaaaaattt ctcattgcaa
    16981 aaagcaagtg aatttcgaga gtcttaaagc aatagcaatt caataccaac ttcagtttcc
    17041 tgtattcaag atcactgaaa agtatattga tatttttcag aattatgtgg ttgaagggga
    17101 taaaaaggcc atttattcac ttcccaaaga gcagcgtgag ttttttaaaa aagagatttt
    17161 gggagaaaag aaggaaggta tttgagaatg tcgtaatttt atgggaatag atgagtttct
    17221 ctggacaatt aatccatttc ccttatgaaa atccgaaaat ggtttcattt aattccagtc
    17281 atacttttaa ccggtatttt ctggaaaacc acgggccaga tcatccacat cccggatgat
    17341 tacccgacga tccagcaagg gatcgatgcc gcgaatcttg acgatacggt gctggtggct
    17401 ccgggtactt attacgagaa cctggagttg attgataaat ggattatcct ggcttctcat
    17461 tttcttacca caaacgatac aagctttatt tcatcaacca taattgacgg tagccatctt
    17521 gggactgtgc ttatcattca taacaacatg ggagccaatg ccaggatttg tggtttttca
    17581 ataaccaatg ggctgggtga tgagggagga ggtattaaga tttcgtcgga atatcccata
    17641 cccgaatacc gtgttattgc aaccctcgat catctaattg tctcgaataa tgaagcggat
    17701 aaaggtggtg gaatttatat ttattggtgt gacccgatcc tggatgattg ccgagtgtat
    17761 gggaattcag catatggctt gggcggaggc atctataccg agaattcttc tgtttcgatg
    17821 aatgcgcttt gcatcagttc taattcttcg ggttacaagg gaggcggtat ttatattaat
    17881 ggcagcagcc cggcacttaa taatttgaca atagccggga attcaaccgg tgctatggga
    17941 ggaggcattt attgttcaag cggatattca atgcctgttc tttgtaattg taatatcaca
    18001 ggtaataatg ccacatcagg tgcgggcatc ggaaatgacg gaaacttatc cgtgaatcaa
    18061 tgccatgttt caggtaatat cgcaacgtgg gaaggtgggg ggatttatac agatgattca
    18121 atcattataa ttaacttggt catagatagt aatacagctg aatatggggg cgggatatgg
    18181 ataaacggag atgcttcgat cgatacgacg gaaataaccg ggaatcatgc aaacgcatat
    18241 gggggtgggc tttatctcac taattcgtcg ggttcaaatc taaacaaggt cctcgtctcg
    18301 gagaatcact cagagtcata tggaggcggc ttctatgctg aaaactctgc agatattaat
    18361 ctgaaggcct ctactatatc gtataacagg tcggaaatat tgggtggagg aatatatcaa
    18421 ttgaatgatc agcagattag ttttgacaat atcaacaggt gtaatatttt tttgaacagg
    18481 gcaccaatcg gtaacgacct ctatttgtat ggtgacaaat tggatgtgat agtcgatacg
    18541 ttcactgttc ttcatccaac ggatcatcat gcgcggtacc tggagaattt tacgtttgat
    18601 attctttcgg gaaaggttca acaggttgaa gccgacctat ttgtttctcc cgaaggcgat
    18661 gatgataatt caggcctaac cccggaagaa cctctgaaaa ccatcaacag ggcgtatgcc
    18721 aaactaatta cggataagga gcatctgcat acggtgtacc tggctgatgg tatttacagc
    18781 caaaccacaa ccggtgaaat tttccccgtc catattatta attacagcag tcttgccggt
    18841 gaatcatgtc cgggaaccat tctggatgcc gagggttctt accatgtcat cagtttatca
    18901 gacgatacgg ttccttcgat aagaaatatg acaatctctg gtgcaatgaa tatttattct
    18961 ggtggcggaa tttcttgcta cacctcacaa gtgtgtatga ataatttgag agtaactgga
    19021 aatttttccg gtgataccgg gggaggaatt gagattagat acggatccaa agtgacgatg
    19081 agcgatatga tcatcgatca tgattcagca cagtatggcg gaggattata cattgataat
    19141 ggcacggaca ctgtgctgat gagtaatgta cagatccttg ataattatgc agcggtgcag
    19201 gggggcggga tttatattta ttttacaaaa ggtttttcac tgatcaattc cagtctggaa
    19261 ggcaacagtt cgaaagccgg tggtggagca tacatggata ggacctcgcc tttaataaaa
    19321 aatacactgt ttgcaaataa ttcggctaat tattatgcag gaggattgga gatctattat
    19381 caattttctg aagacataga gaattcaatt ttgaccaatt gcacatttgc tgataatgag
    19441 aacctgggac tattgtgtta tggtatggga gcgaaattga ccaactgcgt attccgaggc
    19501 aatacaggtg cacaggtcat gttggtcggc ttatggaata atgatacact atttttcgat
    19561 cattcgaatg tcagtggggg tttagatagc attatcgtaa acggatcggc aaaactggaa
    19621 tggctggagg gaaatattgc tgaagatccg catttcgtat tagatggtga ttatccttat
    19681 gccctggccg acgactcgcc atgtatcgat gcgggaacag cagacaccac cggtttattc
    19741 ctgccgctta acgacctggc cggttgtgta cgtgtctggg acggggataa cgatggtact
    19801 accgttatcg atatgggagc gtatgaatat ggcgcaccgg ttggcattac agataagaac
    19861 ccggatctgg ataaaaaaga tggagcttta aggctttacc cgaatccggc aacagacaag
    19921 gttaccgtct ccttttccga tgaccggagc gggcatcggg tcatctttat gaacagtagc
    19981 gggcaagttg ttatgactga gataatctcg tcaggcatta gcaagactga tatatctgtg
    20041 aaaccttttt caactgggat ttatttcgtc aaagcatata gtgaaactgg cgggatagca
    20101 atgggcaaaa tagtagtcct ccgagccaat tggtagaagc atcgaaagtt ttatttctca
    20161 cagatcttca cagatttaag acacagattt cacggatttt atctgtggtg atctgtgccc
    20221 ttaatctgtg ttatctgtga gaaattatat aagaaattat aattcccaga tcatttcacc
    20281 accacctttc gggccaccac cccttcgtcg gtatccaccc gcaccaggta cagccccggc
    20341 gccagcccgt atagcggcac ctcaaccttt tctcccttac aaccataaac ccttaaaacc
    20401 ttacacccca ggttgtcaaa catatcaatg ctttccaccg gctccgtcgc ctcaatgtaa
    20461 agcacaccgg aagcggggtt ggggtagatt ttgacagcat ttcttggttt aatttctgat
    20521 attcctggtc cgctgattag tcccagctca ttggtcttca agaatttgcc tttgacatcc
    20581 ggcaagtctt cgataattcc gacaaccgct aatcctccat ccggacacag ttcaatatcg
    20641 taagcgatcg atttatatcc ttcataatgg taattgtgcc accagagcga atcgccatca
    20701 gcactaaagc agatcaatcc tagatcaaaa ttgccagtat gggcttctcc aatcatccca
    20761 gccatgatat agttattgtt gatatccctg gtaatatcat caaaataccc gccaaagatg
    20821 ttaaacaaat aatgtcgcac ccaaagagtg tcattttcgg gagaaagtcg gctggcataa
    20881 aaccggtcga tcatattatt tccgattgca ataatatcct cttcataatt catcatctca
    20941 taaattgaag cggcatcgta cggactggca atttcatttt tctgtaaaaa agtacctgaa
    21001 tcgctaaacg aatatatggt ttgtccaaaa tgggattcca aagctgaata atagacactt
    21061 gctttaattt taccatccgg catttcgatt atgctcgaag ctattcctga cattattaaa
    21121 tttgaatcga ccatttcaca gtaactttcc catatcttat tcccaaataa atcacattta
    21181 tagagatgaa gatttgcatt tgaagctaaa atgagaaagt tgctatcagc cgtccagatt
    21241 atttcggtca ggccaccaaa gtctgtgacc tttcttgacc agagtgaatc cccatttagg
    21301 ttaaatttct ggatataaat tccgttgttg atttcatatg ccagatagat ttctgcatca
    21361 tttcttgtaa gcataaaagg atcttcatcc ttaagatgtt tttcaggata atactttttt
    21421 gtaaaaatcg tatcaccata caaatccgtt ttgatcaaaa aatagaatcc gccggttctt
    21481 tcgatatggc cggttaaaaa gtatccgtct tccaccggga aaacgtcagt aagcttcatg
    21541 ccttcccaga aatcgtacgt tttgtcgaaa gtgatctgtg ccgggcaaaa actgtatatc
    21601 gtaatcaaaa tccaaaaaac ggttagtctt ttcatatctc aaggttttta tctaattgaa
    21661 tccttgtcaa tccgttttat tcgtgtttta tccgtgttct ttaaatcgga acacggatga
    21721 cacgaatagg acggataatc acggatcatt tcacaaccac ctttcgggcc accaccccgc
    21781 cgccggtttc cacccgcacc aggtacagcc ccggcgccag cccgttaagc ggaatcttca
    21841 atacatgatc tctcgcctgt tcgcccgttc gcccgttcgt ctgttccact gttctattgc
    21901 tcaattgttc aattgttata cctctgcaat caaagatcct cacgctttcg atcttctccg
    21961 aagattcgat gtaaagcacc tccgacgcgg ggttggggta gattttcact gttccggttt
    22021 caacttcctc aataaaagga atctttgtaa tcagaacaca agattccgga gagaattcgg
    22081 actcacaacc gttgtaaaaa agggtttgta ctttatagca atacatacct gttccggagc
    22141 cgaccggatc gatcaattca ttttctatcg tttctgtgat cagttccggc tcacttccgt
    22201 ctatgccgcg ataaacacga taaaagcttt catactgaat gccatccctt tcgaaaaagg
    22261 ctttgatgtt caggttccag ttaaattccg gatttatttc aagtaatgtc gtccattgat
    22321 cgccattacc aaaccggaat aaattgccaa gactgtcaac ggccgggccg ttatcgaccg
    22381 aaatcggata atcacaataa gttgaaatct gataacctac ataaaggtct tttgtaatat
    22441 ctatcttctg cggagtatcg agttgaaaga caccccattg caaagcgtta aggtttccgg
    22501 cagcctgggt gtaaaaaggc acagggtcat cacccgacca aagcgctatt ttaaaacagg
    22561 ctgacacatc ataaggaata aatcctacag cagtaatgct ggcacctttt aattctatca
    22621 ataattcagg agtccagcga atggcaaaat taaaccatga atcttttgaa gtaccaaaac
    22681 caatcccatt ggccggaacc ccgtcatccc actgcctcca ttcggcaatc ggaggtgtgg
    22741 gctctgccca ggtcacctgg acggaatctt ctgatgatgg tattaaagtt gtttcgatgg
    22801 aatcaggagc agggcaaatg cgggttaaag caatattatc cacagcccag taactgatat
    22861 cggaagaatt tacaccggtg gcattgaacc tgatccttaa cctccctgta ttaaatgccc
    22921 cggcgatatc caggctatcc tttcgccagt caaaacttcc ttcggcatta gtaaatgttt
    22981 ttaccgtatt ccatgaccgg tttgtgccat cgaatacctg tacctgcaat ttctcatttc
    23041 cggtgggatt taccgacgat aaagacacgt cgtactcaag gattaactca tgagccgaaa
    23101 gctccaggaa gtggtaactc tctagtgtgg cctcataatc agttattgga gcagcaggca
    23161 taaatacggc gcagggagct tcattaccga tgtcctggct gaccgtccaa ttgctgtcgg
    23221 tgatatacca ggcgttgtca tcgaagttac cggccgacca atcttccagg aaatccagct
    23281 cgaaccaata aatgaataat attatttccg cagggccctc aggcaaagat tcttctgttt
    23341 ccccgggaaa accatacggg gtcatatcat agactgcatt gacttcgtat ttcaggactg
    23401 atccttcata atattcgcta ctactgatcg ggtcgacata accacagcta tcaggagccg
    23461 tataatcgat gtagtcaagg aattcgccgt ttttatagag gttgaaacct aacaggttat
    23521 cggggacaga aggttgcggc ccagggatta atatttcctc aatatccgca agatagattc
    23581 tattctcaac ctggttacat atccagagtg aagtccattg aaaatatccg cttgttttga
    23641 tttcaatgcc ggcacctgca tagggttcac cgcccggaat cataaaagac tcgaattgaa
    23701 tccaattaga gttcggatca atccgatgaa tccaggaagt gcttgcatat gtcgtcaccc
    23761 acaatgttcc cacaggaaaa ttactccccg gagggctcca agccagtccg ctaacattat
    23821 taatgttatg ggttgagata gtatttccgt taaaatgggt cctccagatc atgttactgt
    23881 tccagccgcc gatatagaac tcttcgttct caaaatcagc agccaggccc atctggggtt
    23941 ctacggtgaa gtctccgcct attgtaccga cgatttcacc cgtaggcaga tataccttac
    24001 cgatcctgtt tgatcctcca atcaggcaac aatagagata attaccgtca ctgaccatat
    24061 ccccgatcca tgactggcct tgacttaatg tgattgtctc accggtattt actccatcat
    24121 aagtcacttt gaatatagtt atacctgacc tgttagggtc actcacccac aacccggttc
    24181 catcgtcgca aattccataa catgaagaaa tgggtggcgg agatggaaaa ttccgaagaa
    24241 catcaccgtg ttcacgggcg attgatattg cgggtccatc gtgcatcacc attggtggag
    24301 accatgttaa tatcactgct tcatctggtg ccgaaccacc aagttcagtg ggtttcggaa
    24361 agcaggctga atgcacggat tgctccacag ggtccgagtt gtaattgtta taacaggcca
    24421 tcactgcaca tatatgggtt gtgttatatt caaaactgta ttggaaataa gttgaaggaa
    24481 cacaatcaat tagtgtagta tcaaggaaaa ccttgtaaga gaaggcttga ttggtagttt
    24541 caccggaaat taccgccaca ttatccaccg cccatccgga gccccaccag cccaggttgt
    24601 ccggtcggtc gttggtataa aaggcgagct taaaattgct gattccattg gttccggaaa
    24661 attctgaaag gtcgatctca accctttgcc acaataagga aggttccatt tgtttgagca
    24721 acagccaggt atcgccatca tcaacgctat attttacgta tgctgtttgc ccataggctc
    24781 cgtcaaaata agaatcgaat cgaaggctaa aactatcggc gaaagtcagg tccatttccg
    24841 ggaaatagag aaaatccagg cttccgttat tgttggggat ccaatcatca tttaccaaag
    24901 cgaccttccc ggtatgtggc ggcaccatcc accaggcatg gtaaagagga ttttcatcgc
    24961 attgccagcc actccccagc gtggtatcgg cccatccttc aggcggaaac atgtctcctt
    25021 caaagtcctc attcagtagc acaatcttcg gcgcttccca ggttgccatt aatgtggtgg
    25081 aatcaacccg gaagttgaag ggaggaagga agtcttgctg gcacaggccc tgcaacgcga
    25141 acaacaggaa tatggcaaga gataagagaa gaaaggatag atttttcatc ttcaatcctt
    25201 tatggttaat caatataaat ataggtaaat atatgaaata attgaatcct tgtcaatccg
    25261 ttttattcgt gttttatccg tgttctttaa atcggaacac ggatgacacg aataggacgg
    25321 ataatcacgg atcatttcac aaccaccttt cgggccacca ccccgccgcc ggtttccacc
    25381 cgcaccaggt acagccccgg cgccagcccg ttaagcggaa tcttcaatac atgatctctc
    25441 gcctgttcgc ccgttcgccc gttcgtctgt tcgcctgttc tattgctcga ttgttcaatt
    25501 gttatacctc tgcaatcaaa gatactcact ctttcgatct tctccggaga ttcgatgtaa
    25561 agcacctccg acgcggggtt ggggtagatt gataaaaatg agttattaaa acgatgttca
    25621 ataccgaggc acagatcacc atcttcattt gaaaattcgg attcacaggt atcggtgcca
    25681 ttggtataca gggccgagat cctgtatctg tggtaatcat tttcattttc acaaatggca
    25741 taatcatcca ggaaaaacaa ttgtccggta taggccctca gataatacgg agatccatta
    25801 tcactgcggt agacagcaaa ggtgtcaaat tggtcctgga agacgccaat aggagctgac
    25861 cagcccaggg ccatcgcccc aagtccctga tcatagtttg cttccagctc tgtcggggga
    25921 ttacagttgc tgacaacagc ttcagtagcc aggtatcccg attcgcctgt ttccccgggg
    25981 aatccataag agcttaaatc atatacggca gtaagctgga atgtgtaaat accgggcggg
    26041 gtatcatccg aaatggcgat ttcaatcgta tccgctggcg gctccggtgc gcatgaaata
    26101 tattccacaa aatccatatc cctgtaaagc atcaacccaa gtaaattaaa aggtatgttt
    26161 ccattactat ccaaaggcag acaggctgtc agcatcatat catctgattg gtagaaacat
    26221 gacggcatat aaagataata acggtgaatg acaatattgg aaacgtcata gtccgaccac
    26281 tggccattcg taaaatgcgt tctcacacca tatgcaaaca agccgtcgtc caatgaatcc
    26341 catgcataat cattatattc cgagggcagt atggtatcca gtattaaagt gtcaccttgc
    26401 aatggcggtt cattcatctc aaatcctgaa aaccgggcaa cttcaaaaga atcaacttct
    26461 ctaactggca cattataagc cattacggtg gcacggatca ttaaatcctg caaggggcag
    26521 agcacccacg tgccgctgaa attactgtaa ctcctgaaat atgtaggatt gtcggtatca
    26581 acaccaatgg gtgctgcgtc gggcgatggc gcaagttgct tcatggccag gtaaaagttg
    26641 ctttctgcaa attgagccgt caatccttca aattccaccc acccgtaatt ctcaaccatt
    26701 atgtcagtag aatcgatgac agtaccgggg aagccatcgc tgccatcatc atcataaacc
    26761 agtacacgga aggtggtgcc caaaaatgga ccggggaatg acccgtcacc aacaaaaatc
    26821 cttccgccgg ttacgataaa gttgtcaaaa taagtgatga atttgagtgc attcatgcct
    26881 ccgggattca taaataaaaa atagtcgtct gcttcgccgt cgtcgtacca taattcgggg
    26941 taatagccga ccggatcagg ccattccacc ttgcaccagg tgtcatcatc ctgaactgtt
    27001 gcagtaacag tgaatctcgg ttcatgtgaa acctggcagg gcaattgctt ctgtataaaa
    27061 ctaccgttct gggcaacaac cccgtttatg gcaaacagaa gccaaacgat aaaaaagaat
    27121 gtctttggcg ttttcatcgg tgaattggtt aacattcata agacagaaaa atatatgaaa
    27181 tggttgcatc agtgttcttt aaaccggaac acggatgaca cgaatgggac ggataatcac
    27241 gaatgggacg gataatcccg gatacgaaat tttcctaatg aacgaaaagg taattcacga
    27301 tgaaagcgac cacggtaacg atcatcccga tcataaaaac attgtaggca atcctcacaa
    27361 gccggaactt cttcgacagg atcttcccca gttcatactg gtttttcagc agtaccgaat
    27421 aaagatactc gtcgtctctc atcatatccc tcacggcatt cagatactca tcataatcca
    27481 tcctgatgaa attgccgaag aaattcaggt caacctggtg gttctgaata tcggcgccgg
    27541 aaaatttact tgtcacgatg ttgggccggg tactcaggat cgcaatggtg atggtgacca
    27601 tgcagaccac cagcaacacc aggatcggta tgatatcatt cgacatattg ccgatattgc
    27661 ctgccagcat ggtgataatg atggagatga taatagcgtt gaccgagatc aggatgttgg
    27721 atttgttgtc ggcgatggag ttcaggttga tctggttacg cgccgtgata cggaacatgg
    27781 tatcgacgcc gcgcgaatag ctgaaacgct tttctgaagc cgattctttc cgggcttcga
    27841 tcagcctgcg ccggttcatc cggtcggaca ggcgcctcag cgtttcctcc ttcgcgtctt
    27901 gcaacttggt ttttccgtaa ttggtgtaat attggtgaga tgtcagaaaa tcgacccagc
    27961 gcttttcata aaccggacgc ttttcctgcc ccgggcgcag catcacacac tcgtcgaaca
    28021 gcaggtcgaa ctgctcgatg gcattttcgg atgccaggaa catcaggtcg gcatcacaca
    28081 atgcttcagc aataaggcct tcgggcttcg cttcggcccc agccgacaaa attatccggg
    28141 aaatcaaatc caccgcctcc ggttcaatcc cttcctttac cagaaattcc gcagctattt
    28201 ccgcgctctt ttcacgaacc ttgtctccgc cgccggtata gcccgaataa aggaacaggg
    28261 cacctatccg gagaatattc atctcttctt ccgtcagtcc ctcatgtttt ccaatggttt
    28321 cagcctgctt atccagcttc tgaacatacg ataggttatg aaaaaggacc gtttcgggta
    28381 attgatcagt cagtagaacc gaaacatgct tctcagccgc ggtaaccagg cggtcgtcga
    28441 tttttttcat aaataattga ggataaatga acaaaagtaa ctgaacaaag ttatctaatt
    28501 ttgaggcacg aaacaaaatg ctgacatatt catccgacag atgagaccta cggttctgaa
    28561 cttgttaaaa cactggaaga gggtatctca aaaggattta accacaaagg aacaccaagg
    28621 aattcacaaa ggcacacaaa ggaatcacgt tgacattcaa cgacttcgtg cgcctttgtg
    28681 ttgtactttg cgcgcctttg tggttaattc agacccattt gagtaaaccc taccaattgt
    28741 tttgtaaaga tgggaaccga cgggaagctc gttggactac ttataaaaag taaagattaa
    28801 gactttcgat gcaacttaag atttaatcgg aaaactaaca caagatgaca gatttaacct
    28861 aatttaacag tacgatgatg agaataaccc cggcgataat attgttcctc ctgtttctaa
    28921 ccggagcgga atccttttcc caggataaga atgcaggaaa aacggtgcct ttattcgagt
    28981 cggacgacat cattcatctc gaccttaggg ccgactttaa agccgttttt gctgcaaccg
    29041 acgattcaac ctacttcccg gccgaactta tctggcaaag tgatgacggt aagaatgcgg
    29101 catttgacgt ggagatccgg cagaggggag aaacgcgccg gaaaaagacc acctgttcct
    29161 ttgccccgct gcggctgaaa tttcctaaaa acattaccga tggcacccca ttcgaggatc
    29221 aaaaaacgat caaactggtg acccactgca ataaaggcga tgcattcgaa cagaatatca
    29281 tcgctgagta cctggtatac aaagcattta atgtactgac cgacagctcc ttcaaggtcc
    29341 gcccggccct gatccgctat atctccaccg ggaaaaaggc cgacaccctg cagaaattcg
    29401 ccttctttat cgaacgggag aaacacctgg cccagaggct aaaaggggat gaaatcgaaa
    29461 cggaaaaagt ccatcccgac cggttggatc ctttccagac ctgcctcgtc gatctgttcg
    29521 aatacatgat cggaaatacc gattattcca tctacgaacg gcataatatg gtcattgtca
    29581 gcaataaaac cggtcagcct ccttttatct ccgtgccata cgatttcgac tggagcgggc
    29641 tcgtttcggc cagctatgcc gtgcccaacc ccaaattcaa taccagcgat gtcaaagtac
    29701 ggatctaccg gggactgaaa cgggaccggg aaacggtcga ccgcgccata cggcttttca
    29761 atgaaagaaa acaggaaatt tacagggtgt ttgagtcgta tgagctgctg gagcaaaacg
    29821 aacggaaaca gatcgccagg tacctggatg aattctactg gaccatcaac aacgaagccc
    29881 aggtgagggt ggagtttttt gaaagagcga gggagaggga atagtgaaga gtgaagagtg
    29941 aagtgtgaag tgtgaagagt gaagagtgaa atacatggac aatggacaat ggacaatgca
    30001 caatggacaa tgttaggctc atattgtttt tgctgatttc cggccttttg ttcccgtatg
    30061 tggtttttgg gatgaaagat gcggagaaga cggctgtcaa agcgatcagg gctgacgata
    30121 caaccggttt gcgaatctac atggcatcgc atccgggacc caactgcgag ttcagcaacg
    30181 gtaaaacggg cctgtattac gctattgttt acgacgcaat caattcggct gagttcctgc
    30241 tcgcaggcgg cgctgagccg gatgataccg tcgacgggaa tgcattgctg aaatgggcaa
    30301 taaaatacaa ccggcccagg atcacccggt ttctgatcga attcggggcc gggatcaata
    30361 ccccggatga ctttggaaac acaccgctga tgtatgcggc ttccgtcaat aacctggaga
    30421 tctgccggtt gctggtgaac aggggcgccg atcctatgat taaaaatccc tatggaagga
    30481 gagcttccga ttttgctacg gcggaaagcg aaaccccgtt attccgttac ctgctgttca
    30541 tggagagaat gataatggaa caggattctc tcccggatat gcttgatgga ccttacattt
    30601 ttcaggagaa cgaccgccgg ctcaccatga agtactatga gcggtcggaa gagatgaacc
    30661 ggacctttat ttcggaaaaa acgttaacac cggttattca ggatttggtc gtcagtggat
    30721 ttggatggga tacaaacact taccggataa ccggttcatt tgatccagat tcctgtattt
    30781 ttgaaacaac cggagatgtt ttcgtgatcg gggatattca cggccggtac caggccttag
    30841 tcaatttatt gattaataat aagataatag atgatgaatt gaactggcgt ttcggaaacg
    30901 ggcatctggt tttgctgggt gatgtgtttg accgggggga taaagtgacc gaaaccctgt
    30961 ggttgcttta cgggcttcag tttcaggcaa aggaatcggg cgggaatgtt catcttttac
    31021 tgggcaacca tgaggtcatg gccatgacgg gcgaccaccg gtaccttcac gacaagtacc
    31081 agttttttat gaggtacacc agcagttatt atgatgagct gtacgatgag accagcctgc
    31141 tgggccgctg gctgaggagc cgcaatgtga tcctgcagat caacggtaac ctgtttctcc
    31201 atgccggaat ttcgccggaa ctggcgtcta cgggtttgac ttattcacaa attaattctt
    31261 ctgtgcggga ttatctgtca aaccctaaaa aagttaagaa aggcagcccg gaggaattaa
    31321 ttatgggcga aaatggaccg ctctggttca gggggtatat gaacgcggag ggctatcctt
    31381 cggttcccta ccggtttgtc ggcgaatatc tgcaggtaat gggcctcaac cggatgatcg
    31441 tcggccataa cgagcaacaa tatattaaca cttcatttga gggtaaggtt atttttgccg
    31501 atgtggccat cgatgcaaca gggaaatcgt cgcagggcct tctgatctcc ggtgatgaat
    31561 tttacaggtg ttattcggat gggcgaagag aatccatgcc attgtaaaat ccgcattcct
    31621 gattttaaca ctacattaac tcttcattaa caaatagtta acaatcatgt agatatattt
    31681 gaggttttct caccttaaaa tataagccta catgcaaaca agctgcttta ccgaatctca
    31741 acgacaggcc aaattaccgg aaaatctgaa gtttttactt gctctggtcc tcttaacatt
    31801 atctttcgaa ggattttcac aggtggctat caattctacc ggcctgccac ccgatcccga
    31861 tgccatgctc gatgtatccg ctatcaacca gggggtcctg ataccccgga tactgatcct
    31921 cgacaggccg cctgccaagc cgggcatgct gatgtaccag atcgacggaa tccccggcta
    31981 tgaaccggga ttttattatg ccgacgggca tacctggaac cgggtgggcc attcggcgaa
    32041 tgattactgg ttgccggcgg ttggacccga tatccgtttt ccctacagag tagccctggg
    32101 cggttttacc gatgccgaag atcatggctt gaacgtgcag aactacctgt ccggcaaagc
    32161 cgccgtcagg ggggcggaaa tggatccaaa ctttggaacg ctttatacgg aaggtatgct
    32221 gggggtattg gagccctctc tgctgggcgt gccatttaat gtgcatgatg ccggagtcct
    32281 gggaattgcc aggtcatcct acctaagcag caggggagca gccgtttacg gatggaacaa
    32341 tatgagcgat gctgccgaaa cttacgccgg aatctttatc agcgacggca gttcctttac
    32401 taattacggt gtatattcac aagccagcgt cggaataaca aattacggcc tggaggcccg
    32461 ggctaccg
//
LOCUS       DAOPNH010000006        28580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_1429_length_28580_cov_7.463383, whole genome shotgun sequence.
ACCESSION   DAOPNH010000006 DAOPNH010000000
VERSION     DAOPNH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1429_length_28580_cov_7.463383"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKZ74_00865"
     CDS             complement(<1..833)
                     /locus_tag="PKZ74_00865"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPM91328.1"
                     /translation="MPYLVPVLSGIAACILVPVSGFSLPQVEQAPDTVHAISADTLSA
                     DSLRIDSIAPGKSSDALEYKVDYKSADSTFFDLANKKVYMYRNAEINYDDINLKADYI
                     EIDFNTDEIFAKGLPDSTGKDAGLPVFKQGDQQFESRTMRYNYKSKKGLITSVTTEDG
                     SGFLHGETVKKMDDETTNISSGWYTTCSNKDHPHFAFKYGKSKVVSKKRIVTGPAYLV
                     VEDVPTPLAIPFGWFPNKQGQRSGIILPTYGESAQRGFYFEGMGYYLYINDYLDFKVV
                     G"
     gene            1000..2103
                     /locus_tag="PKZ74_00870"
     CDS             1000..2103
                     /locus_tag="PKZ74_00870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPM91329.1"
                     /translation="MERLKYIFTTLLFVMACAIPTAAQYQIHRVVIDAGHGGKDPGAL
                     GKNSREKDIALAIALKTGDYIKKNHPDVEVIYTRKTDVFVELYKRAKIANDSKANLFI
                     SIHCNASKSTAASGTETFVMGLHKSDAQLEVAKLENAAILNEENFADMYEGFNPNQDE
                     DYITLTMFQDAFLEQSTMLADEIQRQFRERVKRKDRGVFQAGFLVLYKTTMPGVLVET
                     GFITNPEDERFLMSADGQTYIASAIYRAFKYYKEEMERKDNKAQQVVKEYYKEPDAPS
                     PPPEVWFRVQFTSSKTKKVWDSKKYKDMPDIREYRSDGWYKYSTGNFRTYDDAQRHLK
                     FIRETKKFKDAFLICFLKEERISLEKALELIKK"
     gene            2134..3084
                     /locus_tag="PKZ74_00875"
     CDS             2134..3084
                     /locus_tag="PKZ74_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPM91330.1"
                     /translation="MKLSREFRIGFLFILATAILVWGFSFLKNKNILYKETTMYAVYK
                     HVNGLNPSDPVYINGVKVGQVDKVHFDMEKKNGDIIVELNFTENFPIQSNSIARIFSE
                     DLMGSKAVEILLGDSPDFAQAGDTLVTEVETSLKDAVNAQIAPLKLKAEELLSSIDTM
                     VVAIQGIFNREAREDLSASIRSIRHTMNNLESTTQSLDTLLITQSSRLSSIIYNIDMI
                     TRNLNNNSQEINRVLDNLASLSDTIARSNISGIISNLDTTVASLSQVMLRIEKGEGTL
                     GMLINDDQLYKDLEKSASELNLLLEDIRLNPKRYVRVSVF"
     gene            complement(3013..4626)
                     /locus_tag="PKZ74_00880"
     CDS             complement(3013..4626)
                     /locus_tag="PKZ74_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPM91331.1"
                     /translation="MHLNAAHISRNLFTALIIITAALAPVFPLFAQKSTIVHIENADL
                     LRVAKINGMSVKTLKGNAILRQDSTYFYCDTASLDENNNLNAMGRVHINYGDSVHLYG
                     DYLTYSGNTRIAIMDSNVRMVDKRATLYTDHLEYDRNLAVAYYFTGGKIVDDDNVLTS
                     IRGKYLTRLNEFEFSDSVVATHPDYTMYSDTLRYNTETEIVYIEGPTNIYGKEDHIYS
                     EKGWYDTRSDLAELNQNNRIRHLEQILKGDWIYYDRENEYGKARGHVWVMDTVQDIIM
                     EGGIGEFHRSEKYSYITDRARAILIDGSDSLFMHADSFIMVMDSADEARYLFAYHRMK
                     FYKQDLQGLCDSLVYRVQDSVIALLKNPVLWSDDNQLTADSMWMHISDNRIDSMVLFN
                     MAFIVSRDSVETYNQIKGKQMRAYFRDNELYRIKVLGNAETIYYVRDEDGAMIGINKS
                     VSSNMAIMLNDSQVKTISYLSRPEAVLYPEKDLPKEEQFLRDFKWITGKRPYKKDDIF
                     IWERESSEDGNPDVPLRVQPDVLQEEVEF"
     gene            complement(4616..5776)
                     /locus_tag="PKZ74_00885"
     CDS             complement(4616..5776)
                     /locus_tag="PKZ74_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM91332.1"
                     /translation="MKRFSNMKYSFLAAVIVFLASCSAPETEQSIRDRIAANKKKITE
                     LNQEIAGLEKLLGDLNNDDNSSENIAVEVMDLTLSSFSHYIQVSGTAEAVRQAYISPE
                     VSGQIREVYVKEGDYVEKGKLLAKLNSEVTESSIADLQTSLDLAGVVFEKQKRLWEKG
                     IGSEIQFLNAKNNKESLEQKIQTLRAQLDMALIKSPVSGIVDKIYLKKGELAMPGVQL
                     MQVVNLDELYINADVSETYLSDVREGDEVKVEFPVFPDKTLETPIYRKGNVINPNNRT
                     FQIQLKLPNPDKMLKPNILSVIHINDFFADSAIVVPSSIIKQDISGSYLYAIMETGNK
                     WTAKKVYIKTGKSYLDKTMVTDGLKEGMKVITMGYNLVSDGTEVTVKQENAS"
     gene            complement(5780..7123)
                     /locus_tag="PKZ74_00890"
     CDS             complement(5780..7123)
                     /locus_tag="PKZ74_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM91333.1"
                     /translation="MLIIRYFTLITISILLIFNGVLPAQPLSFNLKEAGEYAVKNSYS
                     VQNARYDLEIARKKVKENLSFGFPQIDGTVDYSYFIALPTSLIPGDFFGQPGEMIEVQ
                     FGTKNNLTAGVTLNQLIFDGRYFIGLQYAKIFEQISAENLEKSEEDIRETVSRTYFSI
                     LVGEESLKILDSTLVILMETLYQTEELYKEGFAERTDFDQLKITVTDLQNMINLQRRQ
                     NELGYKLLKYQMGLDLSQEILLTETLDGLINDAGISALAEQQFNPANHIDYRLISTQE
                     KMQVLSLKNERAAYYPKLNGFVFLQENAQRDSFDFFDIDQPWFMTSSTGVSLKIPVFS
                     SGYRKSRVGQAKLELEKIRLTKNQVQAGLELSALQSRSAFITALENYFRDKQNIGLAL
                     DIYSRTLIKYKEGVATSVELTQQHQQFFNAESKYFQTVFNLLDAKNMLDKALGNY"
     gene            complement(7163..7786)
                     /locus_tag="PKZ74_00895"
     CDS             complement(7163..7786)
                     /locus_tag="PKZ74_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91334.1"
                     /translation="MDSTFRGIIEKFRDYLYSMNGKAVVTIEQVAVHTGIPEKVIRSC
                     FSNEAELVEKVLDFERHRFEEIFKIHDFEGVNAIDILLTVSKEVAEKFMDISPSVTFV
                     LKRHFPEIYQQHFILRRDFIYEKIKINLSKGISQGMYRDDLSIELVARLYLSRLIDLH
                     NPDFFPAEEFSFDTLFNVMFENFIRGIAKPEGLSYYEKKKKCFKFNA"
     gene            complement(7874..9436)
                     /locus_tag="PKZ74_00900"
     CDS             complement(7874..9436)
                     /locus_tag="PKZ74_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91335.1"
                     /translation="MKRFALTAILLSGIFLLHAQHQNILITTNASPNEPSIIINPKNT
                     NQLYGGANIASYFYSDDGGYNWQTGTLTSQQYGVWGDPVLLCDTAGAFYFFHLSNPPQ
                     GNWIDRIVCQKVEALGGEWNDGTYMGLDGTKAQDKHWAAVDWKTNNIYVTWTQFDEYG
                     TSDPSKFSNIMFSRSFDGGLSWSPAVQINQVSGDCVDEDNTTEGAVPCVGPEGQIYVS
                     WAGPAGLVFDRSYDQGDTWLDEDIFVSDIGGGWAYDIPGIYRANGLPVTSCDTSSGPY
                     RGTIYINWSDQRNGPGDTDVLLVKSTDGGDTWSDLIRVNDDPPGKHQFFTWMTIDQTN
                     GYLYFVFYDRRNHDNNYTDVYMARSTDGGETFTNFQVSEEYFNPNSNIFFGDYTNITA
                     HDNVIRPVWARCHNNQMSIWTAIVDPDAVGIEEEIAGKPPFSVDRSYPNPFENSTVFS
                     FKLHETAPVRLAVYDSYGRLVEVLADRVILRPGKYTFQFDVRGKNLAAGVYYFSLVSN
                     DKVSRQKVVLAN"
     gene            9500..10018
                     /locus_tag="PKZ74_00905"
     CDS             9500..10018
                     /locus_tag="PKZ74_00905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPM91336.1"
                     /translation="MINLVHGDVKLREFRMEDCHRLVHLANNENISRNLRDGFPHPYT
                     INHAEEFIRNAMMPDPATLFAIEYKEEYVGNIGMVKCKDVYRKSAEVGYFLGEQYWGK
                     GIMTRALGLICEYGFQNLDIVRIHTGVYEHNLASQRVLEKCGFVKEGVFRMAVYKYDR
                     YWNEVRYALIKE"
     gene            10036..10884
                     /locus_tag="PKZ74_00910"
     CDS             10036..10884
                     /locus_tag="PKZ74_00910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM91337.1"
                     /translation="MAKKRKNYKVDSKEVGLELGLRVYKFFLKSEYLHYGYFKDGLEA
                     DVANLRQAQENYAELIFSHIPEGVKSILDVGCGSGKTAQQLVARGYSVDVVSPGQILT
                     AYARKMLGDSVGFFQCRFENLVTDKKYDLVLFSESFQYIPMDKSIPGALKLLNPGGYI
                     IASDFFGDDPERKSKLGGGHNWAEWLKIKETFPVKTLVEKEITAETSYTIDLVNQLNQ
                     EVLAPVWSSAWALDEDRFPLVIRLVKRLYRKKLAKLENKHFTGQRNGEMFRKYKKYMF
                     YLFQSS"
     gene            11072..12577
                     /locus_tag="PKZ74_00915"
     CDS             11072..12577
                     /locus_tag="PKZ74_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM91338.1"
                     /translation="MNKHISLFNAGRILVILVLCTSLRLAAQDPAAGVSDDALIRQTA
                     LNYIEGFYSGDAARMEKAIHPDLNKATPRDLPQTGRTALNYTTWSGLIEFTRAGIGIL
                     ADSARHIGVNILDMGNDVAVVKAISANFTDYLQVVRINDEWKIINVLFASGLKTPARI
                     KDFDPEKERQEIEKAALAYMNALSGADAGRLSQVIDPEYNRVSLVPVGQTGKVTIRRQ
                     RYENVMENALAGVGKQDEMYRYNRVTVLDAFDGIAAVKCESAGATEQIQMYKGDGQWK
                     VFNSIVRPNTAFTLQQAMTATAGEPMPDFSLPVYGGGHFTLSDYKGKNVMLIFPRGWT
                     GQQWCAYCYYQYMEMEQLERTARIMARNDLQIAFVMPYSSDRIRDWQEKFPDAQQVVA
                     GIKNPTPAPAEGTIQAEYAAWARQKFPLTFEVSKDSPHDLIPVLVDENHYLSRQLKLS
                     TNFWDNVTADQNIASVFIIDKKGILRFKYIGQMTEDRPSTGYLLDYIRTMK"
     gene            12672..13928
                     /locus_tag="PKZ74_00920"
     CDS             12672..13928
                     /locus_tag="PKZ74_00920"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM91339.1"
                     /translation="MPERKKVIVIGGGFAGIQFIEHLDNDLFDVLLIDKLNHHQFQPL
                     FYQVATSQLEPSSISFPFRKIFQEHKNMQIRMAEVLRVDPVTNTVITTVGNFRYDFLV
                     IATGCRTNYFGNRNIEENVYSLKTTYEAIKIRNSILQDFEDLLTTPESEKEYLLNIVI
                     VGAGPTGVELAGAFAEIKRNILPKDYPMMDFKDLKIILLEGSGHTLNSMSETSRRVSE
                     KYLRSLGVSVMTNVFVTDYDGKLATLNNGTTIKTTRLIWTAGVTGNVIEGIDKSLIVR
                     NGRIMVDRYNKIRGYDNVFAVGDISYMETLKYPSGHPQVANVAINQARNLARNLRAMI
                     KGKHLKEYEYRNLGSMATIGKNKAVVDLPFIRFHGYFAWFVWMFLHLMLILSIRNKLI
                     VFINWAINYVTKDTSLRLILQEKGKS"
     gene            14025..14678
                     /locus_tag="PKZ74_00925"
     CDS             14025..14678
                     /locus_tag="PKZ74_00925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPM91340.1"
                     /translation="MTTTAALNAQQASGPFELIKLPYAYNALEPHIDAMTMEIHHSKH
                     HAAYVNNLNAAIKGTEAEKMTMEQILGSTKNFDMKVRNNAGGVYNHDLFFTTLSPNGG
                     GKPKGELAKAIDEAFGSFENMKEQMNTAGIGRFGSGWSWLYVTPYGKLAICSTPNQDN
                     PMMDIAENKGVPIFGIDVWEHAYYLKYQNKRADYMNAIWNVVDWNKVAERFEGSKVN"
     gene            complement(14683..15807)
                     /locus_tag="PKZ74_00930"
     CDS             complement(14683..15807)
                     /locus_tag="PKZ74_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91341.1"
                     /translation="MSAKRTFLFSGAIVALSLFLMSWGFQGHYKISSKSAMSFTSEME
                     QFLAWAVTLAEHASDADERKAYDPDEGPRHYIDIDNYPGFEIAGRIPQTYDSVVSLYG
                     EVFVIEQGILPWATLRTYDSLVSCFRRYDWEKAVLFAADLGHYIADGHQPLHLTRNYD
                     GQYTGNSGIHSRYETGMVNTFLNSVDYTGDAISYVEDVENYVFAYIYSNFRYADSIMI
                     ADDYAEAVAGNHSSYTYKQALWEKTGIYTTGFFHDASKSLAELIYSAWTDAGKPDMNS
                     GSGMDEQIRKRDSFRLKARPNPFRETLTISVNLPVAGDICILVIDSRGMIAAKPLDEK
                     REAGEIDIAVNLSRLPDGIYFISVNTGHEFRYQKIIKNSD"
     gene            complement(15815..16348)
                     /locus_tag="PKZ74_00935"
     CDS             complement(15815..16348)
                     /locus_tag="PKZ74_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91342.1"
                     /translation="MIKSKFILICSLLLITYTVAAQDTKPGYDVFSFELAVTVPGTTT
                     QIYDAMTCDISGWWDHSFSEKPYRLYIDAVPGGGFFEIFNAGGDGVKHATVIYSDRGK
                     MLRMDGPLGLSGKAVQMVTTYAFSPTGADSTTVKLSVHAAGEIGDGVPAVVESVWRHF
                     LVERFKPYVENGMKPVE"
     gene            16500..16931
                     /locus_tag="PKZ74_00940"
     CDS             16500..16931
                     /locus_tag="PKZ74_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPM91343.1"
                     /translation="MIYANLPQRIFAQLLDYLVFCIVFFPVTYLVKGTWLMTPEDHLW
                     IIFDPICGVFLIIIFAYFIVLEWLVGFTAGKYLLKMRVVTTEGRKISLKQSLVRNFGR
                     MIDGLPCFNIIGMVSIYKSENRQRVGDKLARTVVICSGKKA"
     gene            16991..17479
                     /locus_tag="PKZ74_00945"
                     /pseudo
     CDS             16991..17479
                     /locus_tag="PKZ74_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            17498..17905
                     /locus_tag="PKZ74_00950"
     CDS             17498..17905
                     /locus_tag="PKZ74_00950"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HPM91344.1"
                     /translation="MEKLSWLAGTWENRSEDGNFIETWRKINDTLYLGESFLVKEKDT
                     VFSEIISLSSASGEILYTVGTSNQNDGRQVPFRLVSDKERIFVFENKSHDFPQEIIYR
                     HPAPDSLHARIQGWVNGEERFQDFPMKRVTSKR"
     gene            complement(17921..21337)
                     /locus_tag="PKZ74_00955"
     CDS             complement(17921..21337)
                     /locus_tag="PKZ74_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91345.1"
                     /translation="MRKILPVFILTLMVITSAAQDTLIVQTFTFDSITTRRGTWQFPE
                     GESFRKILMFHTLKCDFATTHDQYACGEWDYLTYNTVYRHTGAYDSTLYHHPNFTLVE
                     GTAPDSLLLTSSPTYNYFLNRHYHVVFPDTLSFTQATIGNPAVENDKVIPAEYRAGRA
                     QYLWKSTELSAQGLEAGPVTGLKLNLAEGSNGSGDFMIRMKQVSLDELTPDTLIAGLD
                     TVFQNRISFSTGWNDFSFFRPFDWDGVSDIVVEFCYTGSGSSGEVMARAEDPGFNCGI
                     SSAGDNYGLDLDGVSDFLKLQPGTYFNSDFTFETWFFKRNNNNWSRIFDFGNGPDRQN
                     VIVALSTGTSGRLSFHVFMDGLNRSFELDNPTPLYEWTHLAVRLTAHIAWVYINGNLV
                     KLGLLQQPPNLERSINYIGRSNWNGDKAADVLLDEFRLWKTALDPATIKSHCRKGIAD
                     PAADSNLVFYYDFDEGSGNILHDRSINGHHAEGFGRPGFYPVRGPEVYMGFEQDNNRP
                     NIIFERIVSGTVDVSEVTVMDSLMDAPLQIVLFSDPDNPAFPTDTIEKWVAGFKPVYE
                     NWMVVDSVWTAPEEVIRKEMIPYYGEPFEVLEEFEIGRFITPYGIGLTLGDNGFTWVY
                     DVTDYAPLLQGMVDLSAGNQQELIDLKFMLIKGTPPREVKQIDRIWGGLRSFYYKDLD
                     DDLQMSAVTLPLRPDASQFRVKTRLTGHGHNSNTGEYPHCCEWKDNEHYLRVNGQQAG
                     AWHIFQYHDCSLNPVFPQGGTWSGAREGWCPGDLVKDHDFEITDFITGSQVTIDYDIT
                     PVPEDNLGMGWGNYVTNMDLVQYGENHFNVDAEVYNVITPSNVGYYSRVNPVCYGLKA
                     LLRNNGSAPLTSAKFSYGVSGGPQETFDWSGSLQPHTIDTVDLPVSGDLFWIGDENHT
                     FTVTVSEPNGTDDQYTANDTYRSKFNMPDLIDIPIVLELRTNKQAYRFSLEARDMSGN
                     VILRKDNLENTTTYRDTLDLPYGCYTIELTDIEDIGLSYWAYPEQGSGYFRIYDLEGH
                     MIKSFNSEFGRTIFYTYHVGEGFYIGERGFEHLVKIYPNPTDGIVNVDVNDFSGEYLL
                     NIFNMQGVLVRSERIGSGQMVYDLTEFPSGLYLFECKISGFPVFKKVVKR"
     gene            21569..22207
                     /locus_tag="PKZ74_00960"
     CDS             21569..22207
                     /locus_tag="PKZ74_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91346.1"
                     /translation="MKKLLLIVVLVPALFLTSCFKEKAGELGGDQSPMGEVGVTVSSS
                     SMEIAGVSNFTATVTALKDGVSSYTATATVTNPLLKNIAANYPGVEVNGDQVSITNVK
                     IQQTKEGIKSMEGPGAGVLVKYDSKVGDTYPIDGTDRERKVVSKSTDDDYYYGFFLIK
                     TIQVEMDQSHVKSSGITKYTYIANHKFGLVGVKVDFDDQTSVTFPIYTSAEN"
     gene            22241..22684
                     /locus_tag="PKZ74_00965"
     CDS             22241..22684
                     /locus_tag="PKZ74_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91347.1"
                     /translation="MGSFLLPYRWKIAGIVMAIAGMAGAAVYGFFDFRFKVPVFAVVS
                     IFVEPKFFTVFQTNFSEEVVLFFLITGLNLIIFSKEKEEGEDLERIRLKAFARAMITN
                     AAVQLFAVLFVYGAGFAAVMVFNLLSVNILYLIFFYRMRWSLLKN"
     gene            22746..23504
                     /locus_tag="PKZ74_00970"
     CDS             22746..23504
                     /locus_tag="PKZ74_00970"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HPM91348.1"
                     /translation="MNRIIFISLLLITAIIPTWAQTPGTLTVSVSTSAAGGNYAPKNI
                     VAIWIEDNQGNFVKTLLAYAQTRKTHLNTWEASTTAAGSPYNTVDAITGATKTSHATR
                     TCSWDGTDINGNIVSDGTYKLRMELTDKNGTGNFSTFTFEKDTLQVNLTPANVPSFSS
                     IVINWEPVITAIGEPETAGSITIYPNPSSGVINLTGKYDEVIVFGTSGRQIVKSAGNK
                     IDLTASPDGLYFVHVRTGVETEIFKVVKTNDLEK"
     gene            23488..23856
                     /locus_tag="PKZ74_00975"
     CDS             23488..23856
                     /locus_tag="PKZ74_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPM91349.1"
                     /translation="MTSKNKPSPSSDWKSRLGVVYSTNPDYSYATGESAEPGTLPPQQ
                     QNLRVSLDRKQRKGKSVTLIQGFIGRDEDLELLAKQLKSKFGVGGSAKEGEIIIQGDF
                     VVKTIEFLQNLHYKVKRSGG"
     gene            23953..25578
                     /locus_tag="PKZ74_00980"
     CDS             23953..25578
                     /locus_tag="PKZ74_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPM91350.1"
                     /translation="MTKSYLYFLILMIPAGLFAQVSPDWSMIYNGNDDYFDNGVAIAL
                     DPSGNVFVGGTSVSITNGAPDYVVLKYTSAGEFKWSYEYDGTGAYEDFLAAITTDSEG
                     NVYATGGSHEGSSRRDYVTLKIDGSGAFAWKSIYKGLDVNNDDAAADIVVDENGYVYV
                     TGASAGYSSSMWTDFATLKLTPGGDTVWTRRLGGPGMPNDRATAIAVDNFGYVYVTGY
                     FEGDVSTRYNYATAKYKPNGDTAWVRVYNGTGSDEDRAYAIAVDENRNVYVTGSSWGL
                     NADDYATVKYDSNGVQKWVARYNGPAGSYDKAWDLKLDDEGNVYVTGQSGGTSGNYDY
                     CTIRYNPDGGEDWVARYNGPDNLDDVATSVAIDPSGNIFVTGYSYNPPSPYISTTDMV
                     LIKYNPEGLEEWVSRFDGPGNNYDQGYAIAADPSGDVFVTGSTEYYGQQNSRDIFTVK
                     YAGPSLVEDFSSGNSINLYPNPADHQLTVQVPCGAALISVCDLTGRQIIVHELKVSEK
                     DEQTFNISGIPSGAYIVKVTTTLGISEKKLIVY"
     gene            25598..25981
                     /locus_tag="PKZ74_00985"
     CDS             25598..25981
                     /locus_tag="PKZ74_00985"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPM91351.1"
                     /translation="MTAVYHSADEYITCQPEQFRANLALIRQTIREAVPDATEAISYQ
                     MPAFKFHGILCFYAVFKDHYSLFVSPEIHKAFVEELKSYKTTKSAIHFPFDRPVPVEL
                     IVQVVRFAAITNLEKAEARLKKRKR"
     gene            26117..27334
                     /gene="ilvA"
                     /locus_tag="PKZ74_00990"
     CDS             26117..27334
                     /gene="ilvA"
                     /locus_tag="PKZ74_00990"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HPM91352.1"
                     /translation="MLTLPDYSDIIKAQMRLKGVVKNTPLEPSKSFSAMSGAHIYLKL
                     ENFQSTGSFKVRGAYNKISSLDPGELANGVVCASAGNHAQGVAFAARALGLRATVFMP
                     VFTPPLKVIATRSYGADVKLEGETFDDALSAAKKFQESSGAVFIHAFDDPLIIAGQGT
                     IGLELFQALETIQDVVVPVGGGGLIAGIAIALKKTNPDIRIIGVEAEGAASMQTSLRD
                     GKLTRLTSMSTIADGIAVKTPGELTFAAAKQFVDEIVTVSDAEIAHAAYLLLQRAKLL
                     AEPAGVASMAAVLHKKLDFKNRQVVTVISGGNINMGLLEQILEKGMMAEGLRARIAVL
                     IPDMPGELKSVITILENLFANINEISHERSITTVPVGHVLVTITFNLQQIEQLDMVCK
                     ALEGKMKKFEVLR"
     gene            27475..>28580
                     /locus_tag="PKZ74_00995"
     CDS             27475..>28580
                     /locus_tag="PKZ74_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPM91353.1"
                     /translation="MSLKIKLPALHWQILIALALAVLFGIYFSEYVKYVSWMGTIFLR
                     ALQMIIMPLVISSMICGVANIGDAQNLGRIGLKTMLFYVITTVIAILTGLVLVNFFQP
                     GVGADLNLIEKAEIPAASGSLGQILIDIVPENVFAVMSVNRQLLSVILFALLFGFFII
                     KVSEKPRKQLTDFFNAVFEVMMKITFFIIKFTPLGIFGLVAEKIAPHKDVAALFESLG
                     IYMVVVLLGLFIHAVVFMPLIIRFIGRINPLRHFNAVKTALFTAFSTSSSNATLPLTM
                     ESIEVNAGVSNKITSFTLPLGATINMNGTALYECVAVIFIAQAYGIPLSFGQQVIVVA
                     TSLLAAIGAAGVPMAGLVMMSIILSAAGLPLEGIG"
BASE COUNT         7396 a         6804 c         7091 g         7289 t
ORIGIN      
        1 tccccgacaa ctttaaaatc aagataatca ttaatataca gataatatcc catcccttcg
       61 aaataaaacc cccgttgtgc cgattcaccg taagtaggga ggatgatgcc tgaccgttgc
      121 ccctgcttgt tgggaaacca gccgaaagga atagccagcg gcgtagggac atcttccacg
      181 accaggtacg ccggccccgt cacgatcctc ttcttcgaaa cgacctttga ttttccgtat
      241 ttaaaggcga aatgaggatg atctttgttg ctgcatgtcg tgtaccaccc cgaggagatg
      301 ttggtggtct cgtcatccat ctttttcacc gtttcgccat ggaggaaccc gcttccgtct
      361 tccgtagtca cactggtgat caggcctttc ttcgatttgt aattataccg catcgttctg
      421 gattcgaatt gctgatcgcc ctgtttgaag accggcagcc cggcatcttt tccggtagaa
      481 tcgggaagcc ctttggcaaa aatctcatcg gtattgaaat ctatttcgat gtaatcggct
      541 ttcaggttga tatcatcgta attgatctcc gcattccgat acatataaac ctttttattg
      601 gccaggtcga agaaggtaga atcggccgat ttatagtcga ccttgtattc cagggcatcg
      661 gacgattttc cgggagcaat ggaatcgata cgcagggaat cagcggataa ggtatcggca
      721 gaaatagcat gaactgtatc cggtgcctgt tcgacctggg gcagcgaaaa tccggaaacc
      781 ggcaccagga tgcaggccgc gatccctgac aggaccggca ccaggtacgg catcgccgaa
      841 ctttgtcgcc gttttaaaac aaacgctgtt aataatttta tcagcaaagt aaaaatcccg
      901 gaaattatcc ctctaaattt gtattataac ggtttttcct gcaattattg caggttttat
      961 ccgtttttca actcaaagat attcaaactt cccaaaaagg tggaacgctt gaaatatatt
     1021 tttactactc tgcttttcgt gatggcatgt gccattccga cggcagcgca ataccagatc
     1081 caccgggtgg tgatcgacgc cgggcatggg ggcaaagacc ccggtgcgct ggggaaaaac
     1141 tcgagggaga aagacattgc cctggccatt gccttaaaaa caggtgacta catcaaaaag
     1201 aaccatcccg atgtggaggt gatctacacc cgcaagaccg atgtatttgt cgagctttac
     1261 aaacgggcga agattgccaa cgacagcaag gcgaacctgt tcatctcgat ccactgcaat
     1321 gcttcgaaaa gcacggcggc cagcggcacg gagacgtttg tgatggggct tcacaagagc
     1381 gatgcccaac tcgaggtggc caaactggag aatgccgcta tcctcaatga agagaacttc
     1441 gccgacatgt acgaggggtt taatcccaac caggacgagg attacatcac cctgaccatg
     1501 ttccaggacg ctttcctgga gcagagcacg atgctggccg atgagatcca gcggcaattc
     1561 cgcgaaaggg taaagcggaa agaccgcggg gttttccagg ccggtttcct ggtcctttat
     1621 aaaacgacga tgcccggcgt gctggttgaa acaggtttta tcacaaatcc cgaggatgaa
     1681 cggttcctga tgtcagccga cggccagaca tatattgctt cggccattta ccgggctttc
     1741 aagtattaca aggaggagat ggagcggaaa gacaacaagg cgcaacaggt agtgaaagag
     1801 tattacaaag agcccgacgc cccttccccg ccgccggaag tttggttcag ggtgcagttc
     1861 acatcttcca aaaccaaaaa agtctgggac agcaaaaaat ataaagatat gccggatatc
     1921 cgggagtaca ggtccgacgg ctggtataag tattctaccg gcaatttcag gacttatgac
     1981 gatgcccagc ggcatcttaa atttatccgg gagacgaaaa aatttaaaga cgcctttctc
     2041 atctgtttcc tgaaagagga gcggatttct ttggaaaaag cccttgaatt gatcaaaaag
     2101 taattatatt tgttttcatc aaaaacgacc attttgaaac ttagccggga attcaggatc
     2161 ggttttctct ttattcttgc cacagcgata ctggtgtggg gttttagttt tttaaaaaac
     2221 aaaaacatcc tttacaagga gaccacgatg tatgccgttt acaagcatgt gaacgggctg
     2281 aacccgtccg atcccgttta tatcaatggc gttaaggtcg ggcaggtgga taaggtccat
     2341 tttgatatgg aaaagaaaaa tggcgatatc attgtcgaac ttaatttcac cgaaaatttc
     2401 ccgatccagt cgaattcgat tgccaggata ttttcagaag acctgatggg ttcgaaagcc
     2461 gtcgagatcc tgctgggcga cagtcctgac tttgcgcagg ccggcgatac cctggttact
     2521 gaagtcgaaa catcgctgaa agatgccgtg aacgcacaga ttgcaccgct caagctgaaa
     2581 gcggaggagc tcctttcgtc gatcgatacg atggtggtgg ctatccaggg cattttcaac
     2641 cgcgaggcgc gcgaagacct gtcggccagc atccgcagca tccggcatac gatgaataac
     2701 ctggaaagca cgacacaaag cctggatacg cttctgatca cccaatccag ccggttatcg
     2761 agcatcatct acaacatcga catgatcacg cgcaatctca acaacaacag ccaggagatc
     2821 aaccgcgtgc tcgacaacct ggcatcactg agcgacacaa tcgcccgctc caacatttcc
     2881 gggatcattt ccaatctgga tactacagtg gccagcctgt cgcaggtgat gcttcggatt
     2941 gaaaaaggcg aagggacgct ggggatgctg atcaacgacg accagcttta caaggacctt
     3001 gagaaatcgg cttcagaact caacctcctc ctggaggaca tccggctgaa cccgaagcgg
     3061 tacgtcaggg tttccgtctt ctgagctctc tctttcccag ataaagatat catctttttt
     3121 atagggcctt ttgcccgtaa tccatttgaa atcacgcaga aactgttctt ctttcggaag
     3181 gtctttttcc ggatacagga ccgcttcagg acgggaaagg taactgatgg tcttcacctg
     3241 gctgtcgttg agcattattg ccatattact tgaaaccgat ttattgatcc cgatcatggc
     3301 tccgtcttca tccctgacgt aatagatcgt ttcggcattg cccaggactt tgatccggta
     3361 tagctcatta tccctgaaat aggctctcat ctgcttgcct tttatctggt tataggtttc
     3421 gacgctgtcg cgcgacacga taaaagccat gttgaacagt accatcgaat cgatgcggtt
     3481 atcgctgatg tgcatccaca tcgaatcggc ggtgagctgg ttatcgtcag accacagtac
     3541 cgggttcttc aaaagggcaa tgaccgaatc ctgcaccctg tagaccagcg agtcgcacaa
     3601 gccctgcagg tcctgtttgt aaaacttcat ccggtggtaa gcaaacaggt accttgcctc
     3661 atcggccgaa tccatcacca tgataaagct gtcggcatgc atgaacagcg aatcactccc
     3721 atcgatcagg atggcgcggg cccggtcggt gatataagaa tacttttcgc tgcggtgaaa
     3781 ttcaccgatg ccgccttcca tgattatatc ctgcactgta tccatgaccc agacatggcc
     3841 cctggctttg ccgtattcat tctcccggtc atagtagatc cagtcgcctt tcaggatctg
     3901 ttccaggtgc ctgatgcggt tgttctggtt tagctcggcc aggtcactgc gggtatcata
     3961 ccatcctttc tccgaataaa tatgatcctc tttaccataa atattggtcg ggccttcgat
     4021 ataaacaatc tccgtttcgg tattgtaacg gagggtatcc gaatacatcg tataatcggg
     4081 atgggtagcc accaccgaat cgctgaactc aaattcattg agacgtgtaa ggtattttcc
     4141 gcggatgctg gtcagcacat tgtcgtcgtc gacaatcttt ccgccggtaa aatagtaggc
     4201 tacagccagg tttcggtcgt attccaggtg atcggtatac agggttgccc ttttgtcgac
     4261 catcctgaca ttggagtcca tgattgcgat ccgggtattg cccgaatagg taaggtagtc
     4321 gccatacaga tggaccgaat cgccatagtt aatatgtacg cgccccatgg cattcaggtt
     4381 gttattttca tccagcgagg cggtatcgca gtagaagtag gtgctgtcct gcctgagtat
     4441 cgcattacct ttcaacgttt ttacgctcat tccattgatc ttggctacgc gaagcaggtc
     4501 agcattttcg atgtgaacga tggtcgattt ctgggcgaac aaagggaaaa caggggctaa
     4561 agccgctgtt attatgatca gagcggtaaa taaatttctg ctgatgtggg ccgcattaag
     4621 atgcattctc ttgttttacg gtaacttcag ttccatccga caccaggtta tatcccatgg
     4681 tgatcacctt catcccttcc ttcagcccgt cggtcaccat tgttttgtcg aggtaagact
     4741 ttccggtttt tatataaact ttcttcgccg tccatttatt gcctgtttcc atgatcgcgt
     4801 aaagatatga tcccgaaata tcttgtttta taatggaaga tggcacgacg atggccgaat
     4861 cggcaaaaaa atcattgata tggatgacgg agaggatatt cggtttcagc attttatccg
     4921 gatttggaag cttcaactgg atttgaaagg tcctgttgtt tggattgatg acgtttcctt
     4981 tccggtaaat gggagtttca agggttttat cggggaaaac ggggaattcc accttcactt
     5041 cgtcaccttc cctgacatcg ctgagataag tttccgacac atcggcattg atataaagct
     5101 catcgaggtt gaccacctgc atcaactgca cgccgggcat ggccagctca ccctttttca
     5161 ggtagatctt atcgacgatg ccgcttaccg gcgacttgat cagggccata tcgagctggg
     5221 ccctgagcgt ctggatcttt tgttcgagcg attctttgtt gtttttagcg ttcaggaact
     5281 gaatttcgga accgattcct ttctcccaaa gccgcttctg cttttcaaaa acgacgccgg
     5341 ccaggtcgag cgaagtctga agatcggcga tggagctttc ggtcacctcc gagttcagct
     5401 tggccaataa tttacctttt tcgacataat cgccttcttt gacataaact tccctgatct
     5461 gaccactgac ttcaggagaa atataagcct gcctgaccgc ttcggctgtt ccgctcacct
     5521 ggatataatg gctgaaagag ctcagggtga ggtccataac ttcgacggct atgttctctg
     5581 aactgttatc gtcgttgttt aaatcgccaa gtagtttctc cagcccggca atctcctggt
     5641 tgagttcagt gatcttcttt ttgttagcgg caatacggtc ccggatcgat tgttcggttt
     5701 cgggagcgct gcacgaagcg agaaaaacaa ttaccgctgc taaaaaactg tatttcatgt
     5761 ttgaaaatct tttcaagttt taataattac ccagggcttt atccagcatg tttttcgcgt
     5821 caagcaggtt aaaaacggtc tggaaatatt tactttcggc gttgaagaac tgctgatgct
     5881 gctgggtcag ttccacgctg gtagcaaccc cttctttgta tttaatgaga gtccggctgt
     5941 aaatatctag agccaggcca atgttttgct tatccctgaa ataattctcc agtgcggtga
     6001 tgaatgccga tctggattgt aaggctgaca gttccagccc ggcctgaacc tggttttttg
     6061 ttaaccggat cttttccagc tcaagttttg cctgtccgac ccggcttttc cggtaaccgc
     6121 ttgaaaagac aggtattttc aaagaaacgc cggtggacga ggtcataaac cagggctggt
     6181 cgatgtcaaa aaaatcgaaa gaatcgcgct gggcgttttc ctgcaaaaag acgaagccat
     6241 tgagcttggg ataataagcg gctctttcgt tcttcaggct tagcacctgc attttttcct
     6301 gtgttgagat caaccggtaa tcgatgtggt ttgccgggtt gaactgctgt tcggccagcg
     6361 cgctgatgcc cgcatcgtta atgagtccat caagtgtttc cgtcagcagg atttcctggc
     6421 tcaggtccag gcccatctgg tatttaagca gtttatatcc gagttcgttc tggcgcctct
     6481 gcaaattgat catattctgc agatcggtta ccgtgatctt tagctggtcg aaatcggtcc
     6541 tctcggcaaa tccctccttg tacaattctt cggtctgata aagtgtttcc atcagaatga
     6601 ccagcgtgga atcaagaatt ttgagcgatt cttcgccgac caggatgctg aaataggtcc
     6661 gggaaacggt ttcgcggata tcctcctccg atttctccag gttttcggcg ctgatctgct
     6721 caaaaatctt tgcatactgc aaaccgataa aataccgtcc gtcgaagatg agctggttga
     6781 gcgtaacacc tgccgtcagg ttatttttag tgccgaactg tacctcgatc atctcaccgg
     6841 gctgcccgaa gaagtcgccc ggaataagtg atgtaggcag tgcgatgaaa taagaatagt
     6901 cgaccgtacc gtcgatctgg ggaaatccaa aggaaaggtt ttctttcacc tttttccggg
     6961 ctatttccag gtcataacgg gcattctgaa cactgtaact gttttttacg gcatattcac
     7021 cggcttcctt caggttgaaa gaaagtggct gggctggaag aaccccattg aatatcaata
     7081 gtatagatat tgttattaat gtaaaatatc ttataatcaa cattaaccgt tttttttgat
     7141 cgattgtcat gggattggca atctatgcat tgaacttaaa acactttttt ttcttctcgt
     7201 aatagctgag cccttccggt ttggcgatcc cgcggataaa attttcaaac atcacattga
     7261 aaagagtatc aaatgaaaat tcttccgccg ggaaaaaatc ggggttatgc aggtcgatca
     7321 ggcggctgag gtaaaggcgg gcaaccagtt cgatactgag atcgtcacgg tacattccct
     7381 ggctgatccc tttcgacagg ttgatcttga tcttttcata aataaaatcc cgccgtagga
     7441 taaaatgctg ctggtatatt tccggaaaat gacgtttcag cacgaatgtc accgaagggg
     7501 agatatccat gaatttctct gctacttcct tgctgacagt caataggata tcgatagcat
     7561 taactccctc gaaatcatgt attttaaata tctcttcaaa ccggtgtctt tcaaaatcaa
     7621 gtaccttttc gaccaattcc gcctcattcg aaaaacagga acggatcacc ttttcaggga
     7681 tgccggtatg aacggcgact tgttcgatgg taacgacagc tttgccgttc atcgaataca
     7741 gataatccct gaatttctca atgatcccac gaaaggtcga atccataagg aaggttgttt
     7801 ttgaaagcgg cgcaaaatta ctaattctgc tgaagtaaca aaagaaaata aatccggttc
     7861 actgacgaaa aaattaattt gccagtacaa ctttttgccg gctgacctta tcattgctga
     7921 ccaggctgaa gtaataaacc ccggcagcga ggtttttgcc tctcacatca aactggaagg
     7981 tatattttcc gggccggaga atgacacggt cggccaggac ctccaccagc cggccatagg
     8041 aatcgtaaac ggccagtctg acaggcgctg tttcgtgcag cttgaaagaa aaaacggtcg
     8101 aattctcgaa ggggttggga tagctccggt cgacgctgaa tggtggcttt ccagcaatct
     8161 cttcctcgat gccgacggca tcgggatcga cgatggcggt ccagatgctc atctggttgt
     8221 tgtggcaccg ggcccaaacc gggcggatca cgttatcatg tgccgtaata ttggtataat
     8281 cgccaaagaa aatattgctg ttgggattga agtactcttc gctgacctgg aaattggtga
     8341 acgtttctcc tccatcggtc gagcgggcca tgtaaacgtc ggtatagttg ttatcatggt
     8401 ttcggcggtc atagaagacg aaatacaggt aaccgttggt ctggtcgatg gtcatccagg
     8461 taaagaactg gtgtttgccg ggggggtcgt cgttgacgcg gatgaggtcg ctccaggtat
     8521 ctccgccgtc ggtcgatttg acaagcaata cgtcggtatc gccagggccg ttgcgctgat
     8581 cgctccagtt gatgtagatg gttccgcggt acgggccgct gctggtgtcg cagctcgtta
     8641 cgggaaggcc gttggccctg tagatgccgg gaatatcgta ggcccatccc ccgccgatgt
     8701 cggaaacgaa aatatcctca tccagccagg tgtcgccctg gtcgtaggaa cggtcgaata
     8761 ccagtccagc cggtccggcc catgagacat agatctggcc ttccgggcca acgcagggca
     8821 ccgctccttc tgtcgtatta tcttcatcga cacaatcgcc cgaaacctgg ttgatctgaa
     8881 ccgccgggct ccagctcagg ccaccgtcaa atgaacggga gaacatgatg ttactgaatt
     8941 ttgaaggatc ggaagtgccg tattcgtcaa actgcgtcca ggtaacgtaa atattgttgg
     9001 ttttccagtc gactgcggcc cagtgtttat cctgcgcttt ggtgccgtcg agccccatgt
     9061 aagtcccgtc gttccattca cctcccaggg cttcaacttt ctggcaaacg atccggtcga
     9121 tccagtttcc ctgtggcgga ttggagaggt gaaaaaagta gaaagcgccg gcggtgtcgc
     9181 acagcaggac cgggtcgccc cagacgccgt actgctggga tgtcagcgtg ccggtttgcc
     9241 agttgtatcc tccgtcgtcc gaataaaaat agctcgcgat attggcgcca ccgtaaagtt
     9301 ggttggtgtt cttcgggttg atgatgatcg aaggctcgtt gggtgaagca ttcgtggtga
     9361 tcaggatatt ctggtgctgg gcgtgcaaca aaaaaatccc cgataacagg atggcagtta
     9421 aagcaaatct tttcatgatg ggttcaatga catcacaaag atattatttt cctacttttg
     9481 attgatttta atgtcgatta tgataaacct tgttcatggt gatgtaaaac tccgcgaatt
     9541 ccgtatggaa gattgccaca ggcttgtgca cctggctaac aatgaaaaca tcagccgtaa
     9601 cctgcgcgac ggttttcccc atccatatac gataaaccat gcggaagagt ttatccgcaa
     9661 tgccatgatg cctgatccgg cgactctctt tgccattgag tacaaagagg aatatgtcgg
     9721 gaatataggc atggtaaaat gcaaggatgt ttacaggaaa tcggccgaag tgggttattt
     9781 tctgggggag caatactggg gaaaaggaat tatgacccgg gcgctggggc tgatctgcga
     9841 gtacggtttt caaaacctcg atattgtcag gatccatacg ggtgtttatg agcacaatct
     9901 cgcatcgcaa agggtactgg aaaaatgcgg attcgttaaa gaaggtgttt tccggatggc
     9961 tgtttacaaa tatgaccggt actggaatga agtcaggtac gccctcatca aagaataaac
    10021 tatatcattg acttaatggc aaaaaaacga aaaaattaca aggtcgactc gaaagaggtc
    10081 ggactggaac tgggattaag ggtttacaaa ttctttctga aatcggaata tctgcattac
    10141 ggttatttta aagacggcct ggaagccgat gtggcaaatc tccgccaggc ccaggagaac
    10201 tatgcggaac tgatcttttc acatatcccg gaaggggtaa aatcgatcct tgacgtcggt
    10261 tgcggatcag gcaaaaccgc tcaacaactg gtagcccggg gctattcggt cgatgtggta
    10321 tcacccgggc agatcctgac tgcttatgcc cgtaaaatgc tgggtgattc ggtgggattc
    10381 tttcaatgcc ggtttgaaaa cctggtaacc gataaaaaat acgacctggt actcttcagt
    10441 gagagcttcc agtatatacc gatggacaaa tccatccctg gggcgcttaa gctgctgaat
    10501 cccggcgggt atataattgc cagtgatttt ttcggcgacg atcccgaaag gaaatcaaag
    10561 ttgggtggcg ggcacaactg ggccgaatgg cttaaaatca aggaaacttt tcccgtgaaa
    10621 acgctggttg aaaaagagat cacggcagag acatcctaca cgatagattt ggtcaatcag
    10681 ctaaaccagg aggtgctggc gccggtctgg tcgtcggcat gggcgctcga cgaagaccgt
    10741 tttccgctgg taatcaggct ggttaaacga ttgtaccgga aaaaactcgc caaattggaa
    10801 aacaagcact tcaccggcca gcgtaacggc gagatgttca ggaaatataa gaagtacatg
    10861 ttttatttgt tccaaagttc ctgagttccg ggttccgggt tccaggttcc gggttccggg
    10921 ttccaggttc cgggttccag gttccgggtt ccgggttctt ttcttttgaa attggctgag
    10981 gtttaccttt tgtattgtca ttcgtgccaa ttatagaatt ccccttctga taatatgatg
    11041 attggtttag atttatgaca aaaatattgt catgaataaa catatatcgc ttttcaatgc
    11101 cggccggatc ttagtgatcc tggtcctttg cacatcgctc cgtttagccg cgcaggatcc
    11161 cgctgcaggg gtgtcagatg atgccttgat caggcaaacg gctttgaatt acatcgaggg
    11221 attttactcc ggcgacgctg cccggatgga aaaagccatc catcccgacc tgaacaaggc
    11281 aactccgcgg gatttgcccc agaccgggcg cacagcgctc aattacacca catggtcggg
    11341 cctgattgag tttacccgcg ccggcatcgg catcctggcc gacagtgccc gccatatcgg
    11401 ggtcaatatc ctcgatatgg gaaatgatgt ggcggtggtc aaggcaatat ccgccaattt
    11461 caccgattac ttgcaggtgg tcaggattaa cgatgaatgg aaaattatca acgttttatt
    11521 tgcttcgggg ctgaaaacgc ctgcccgcat caaagatttc gatccggaaa aagagagaca
    11581 ggaaattgag aaagcggccc tcgcctacat gaatgctttg agtggagccg atgccggccg
    11641 cctgtcgcag gtaatcgacc cggagtacaa ccgggtgagc ctggttccgg taggccagac
    11701 cggcaaggtg accatccgca ggcagcgata tgagaatgtg atggagaatg ccctggcagg
    11761 ggtcggcaag caggacgaaa tgtaccgtta caaccgggtt acggtgctgg acgcgttcga
    11821 tgggattgcc gccgtaaaat gtgaatcggc cggtgccacc gaacagatcc agatgtataa
    11881 aggcgacggt caatggaagg tattcaacag cattgtcagg cccaatacgg cttttacgct
    11941 gcagcaggcc atgacggcaa ccgccgggga accgatgccc gatttttcgt tgccggttta
    12001 tggtggcgga catttcacac tgtcggacta taaagggaaa aatgtgatgc tgatctttcc
    12061 gcgcggatgg acgggccagc agtggtgcgc ttactgctat taccagtaca tggaaatgga
    12121 acagcttgaa cgaacggccc gcatcatggc ccgcaacgac ctgcagatcg ctttcgtaat
    12181 gccttacagc agcgatcgga tcagggactg gcaggaaaag tttccggacg cgcagcaggt
    12241 agttgcaggt attaagaatc ccacaccggc ccctgccgaa ggaaccatcc aggctgaata
    12301 tgccgcgtgg gcccgccaga aattcccgct gacttttgag gtcagcaaag attcgccaca
    12361 cgatttgatc ccggtgctgg tggatgaaaa ccactacctg tcgaggcaac tgaaattatc
    12421 tacgaacttc tgggataatg tgacggcgga tcagaatatc gcatcggttt tcattattga
    12481 taaaaaaggg atcctacgtt tcaaatatat cggccagatg acggaagacc ggccctccac
    12541 cggctacctg ctggattaca tccggacgat gaaatgatcc tttgatttgt ggtggaacgg
    12601 tggaatggtg gaatggtgga acagtttttt gtttccgcta tctttgcagc gggatataat
    12661 tccgtcgggc catgccagaa agaaagaaag tcattgtaat cggcgggggt tttgccggta
    12721 tacaatttat cgaacacctt gacaatgatc tgttcgatgt tttgctgatc gacaagctga
    12781 accatcacca gtttcagccg ttgttttacc aggtggcgac gtcgcagctt gaaccgagca
    12841 gcatctcttt tcccttccgg aaaatcttcc aggaacataa aaacatgcag atcaggatgg
    12901 ctgaggtgct acgggtggat cctgttacga acaccgtgat cacaacggtc ggcaatttca
    12961 ggtacgattt cctggtgatc gctaccggtt gccggaccaa ttatttcggt aaccggaaca
    13021 ttgaggaaaa tgtctattca ctcaaaacga cttacgaggc gataaagatc aggaacagca
    13081 tcctccagga ttttgaagat ctgctcacca cacccgaatc ggaaaaggag tatttgctga
    13141 atattgtcat tgtcggggcc ggcccgactg gcgttgaact ggcgggtgcc ttcgccgaga
    13201 tcaagaggaa tatcctcccg aaggattacc cgatgatgga tttcaaagat cttaaaataa
    13261 tcctgctgga agggagcgga catacgctga acagcatgag cgagacgtcc cggagagtat
    13321 cggagaagta tctcagatcg ctgggggtca gcgtcatgac caatgtcttt gtgaccgatt
    13381 acgatggtaa attagcgacc ctgaataatg gtacaaccat caaaaccacg cggttgatct
    13441 ggacagccgg cgtaacgggc aatgtcatcg aaggaatcga caaatcactg attgtcagga
    13501 atggcaggat aatggtcgac cggtacaata agatccgggg gtacgataat gtttttgccg
    13561 tcggcgatat ttcttacatg gaaacattga aatacccttc cggacatccg caggtggcca
    13621 acgtggctat caaccaggcg cgaaacctgg cccgcaacct gagagccatg atcaaaggaa
    13681 aacatctcaa agaatacgaa taccgcaacc tgggctcaat ggcaaccatc ggaaagaaca
    13741 aggcggtcgt cgatctgccg ttcatccgtt tccacggtta ttttgcctgg tttgtctgga
    13801 tgttcctcca cctgatgctg atcctgagca tccgtaacaa gctgatcgta tttatcaact
    13861 gggctatcaa ttacgttacg aaagacacat cgctccgttt gatattgcag gagaaaggga
    13921 aatcataaaa atttgaacaa attgtatcat catctgtttt caataatgat taaaaaccag
    13981 taacaatgaa aaaaacatca atcttacaat ttatgatcat tatgatgacc acaaccgcag
    14041 cattaaatgc acagcaggct tccgggcctt ttgaactgat aaaactgcct tacgcttaca
    14101 acgcacttga gccgcatatc gatgcgatga cgatggaaat tcatcactcg aagcatcacg
    14161 cagcctatgt aaataatctg aatgccgcta tcaaagggac agaagctgaa aaaatgacga
    14221 tggaacaaat cctgggcagt accaagaact tcgatatgaa ggtaaggaac aatgccggcg
    14281 gggtttacaa ccacgacctg ttttttacca ccctttctcc aaacggtggc ggcaaaccta
    14341 aaggcgagct ggctaaagct attgatgaag cattcggaag ctttgagaat atgaaagagc
    14401 agatgaacac cgccggtatc ggccgtttcg gctcaggctg gtcatggctc tatgttacac
    14461 cctacggaaa actggccatt tgttccacgc ccaaccagga caatccgatg atggatatcg
    14521 ctgaaaacaa aggagttcct attttcggga tcgatgtatg ggaacatgcc tattacctca
    14581 agtaccagaa caagcgtgcc gattacatga acgccatctg gaacgtggtc gactggaata
    14641 aagtagcgga gcggttcgaa gggtcaaaag tgaactgatc gcttagtcgc tgtttttaat
    14701 gattttctga taccggaatt cgtgtccggt attgacggat ataaaataaa tcccatccgg
    14761 caggcgggag aggttgacag cgatgtcgat ctctcccgcc tctctttttt catccagcgg
    14821 tttagcggca atcatgcccc gggaatcgat gaccaggata cagatatcgc ctgctaccgg
    14881 gaggtttacg ctgatggtaa gcgtttccct gaaagggttt ggcctggctt tcaaccggaa
    14941 cgaatcccgt ttgcggatct gttcatccat gccggaaccg ctgttcatat ccggctttcc
    15001 cgcatcggtc caggctgaat agatcaactc ggccagcgat ttcgaggcat catggaaaaa
    15061 tccggtcgtg taaatgcccg ttttctccca cagtgcctgt ttatatgtat aggatgagtg
    15121 attaccggcc acggcctcgg cataatcgtc ggcaatcata atggagtcgg cgtaacggaa
    15181 attggaatag atgtaagcga agacgtagtt ttccacatcc tccacatagg agatggcatc
    15241 tcccgtgtaa tcgacggagt tcaggaaagt attgaccatg ccggtttcat agcgggaatg
    15301 gatgccgctg ttcccggtgt attgcccgtc gtagttccgg gtgaggtgta acggctggtg
    15361 gccgtcggca atatagtgac ccaggtcggc ggcgaaaaga acggcttttt cccagtcata
    15421 ccgcctgaag cagctcacga gggagtcgta ggtccggagg gtggcccagg gaagaatgcc
    15481 ctgttcgatc acgaacacct cgccgtaaag ggaaacgaca gagtcgtagg tttgcggaat
    15541 gcgcccagcg atttcaaatc cgggatagtt atcgatatcg atgtaatggc ggggcccttc
    15601 atcgggatcg taagcttttc tttcgtcggc gtcggaggca tgctcggcca gcgtgacggc
    15661 ccaggccagg aactgctcca tttctgaagt gaacgacatg gcagacttgc tgctgatctt
    15721 ataatggccc tggaatcccc acgacataag gaaaagggag agggcgacga tggctcctga
    15781 aaacaggaag gttctttttg cgctcatagt gagattattc taccggtttc atcccgtttt
    15841 cgacatatgg tttaaagcgt tcgacaagga aatgccgcca gaccgattca accacggccg
    15901 ggacgccatc ccctatctca cccgccgcat ggacggaaag ttttaccgtt gtggaatcgg
    15961 cacctgttgg agaaaaagca taagtggtca ccatttgcac cgctttgccg gaaagtccca
    16021 gcggcccgtc catccgcagc atcttacccc ggtcggaata gatgacggtg gcatgtttca
    16081 cgccatcgcc accggcatta aaaatctcaa aaaatcctcc acccggaact gcatcgatat
    16141 aaagccggta gggcttttcg gaaaacgaat gatcccacca tccgctgata tcgcaggtca
    16201 tggcatcata gatctgcgtc gtggtgcccg gcaccgtgac tgccagttca aatgaaaaca
    16261 catcataacc gggttttgta tcctgggccg caacggtata cgttattagc aggaggctac
    16321 agataaggat aaacttcgat tttatcatgt aatgaatgta gattaaaact gcaaaaatag
    16381 atttaaaaca aacaggttaa tttttcctgc ccaattaggg acgccatccg ccggctggcg
    16441 gatggcgtcc cttttttttc taccttcgtt ataaattact atcaacttca gaaatcaata
    16501 tgatctacgc taatctgcct caacgcatct tcgcccagct tctcgactac ctggtctttt
    16561 gcatcgtctt ttttcccgta acctacctgg taaaaggcac ctggctgatg acgcccgaag
    16621 accatctctg gatcatcttc gatccgatct gcggggtgtt cctgatcatt atcttcgcct
    16681 acttcattgt actcgaatgg cttgtgggtt ttaccgccgg gaaatacttg ctcaaaatgc
    16741 gggtcgtcac aaccgaagga cggaaaatca gcctgaaaca aagcctggtc cgcaatttcg
    16801 gccggatgat cgacgggctg ccgtgcttta atattatcgg gatggtcagt atttataaat
    16861 cggaaaacag acaacgggtt ggggacaaac tggcacggac tgttgtgata tgttcgggga
    16921 agaaagcgta gttattattt tttaaggaag aaatatgaac aaaactattg tcagaaacat
    16981 cctgattata atgatgatgg tcattaattg catgaccacc gtcagccagc ccgacgaccg
    17041 cggatatatt gtaaaaaccg gcgaaacagc tcccgacttt acggtaacca caacggaagg
    17101 aaaagtattc acgctgtcgg aacagagagg caaaatagtc atgctgcagt tcacggccag
    17161 ctggtgcggc gtctgccgga aagagatgcc gtatatcgag cgggagatct ggcagccgct
    17221 gagagacaat gactttgtgc ttattggcct ggaccgcgat gaaccggcag atgtggtcag
    17281 ggaatttgca tcgaaaaccg gtgttaccta tccattggcg cccgatccgg cagcggcagt
    17341 tttttctttg tatgcagaga aacaggcagg catcacccgg aatgtgatca tcgaccggga
    17401 cggaaagatc gtttacctga cccggttatt cgacctggaa gaattctatg gaatgaaaga
    17461 aaccatctgg gaactgatgg ggcttccgct caccggcatg gaaaagctgt catggctggc
    17521 cggcacatgg gaaaaccggt cggaagacgg aaatttcatc gaaacctggc gaaagatcaa
    17581 tgataccctg tacctcggag aaagtttcct ggtcaaggaa aaggatacgg tgttttcaga
    17641 aataatcagc ctgagctccg cttccggcga gatcctgtat acagtcggca ccagcaacca
    17701 gaatgatggc cggcaggtac ctttccggct ggtttcagat aaagaacgga tcttcgtgtt
    17761 tgaaaataaa tcccatgatt tcccgcagga gatcatttac cgccatcctg cacccgattc
    17821 cctgcacgcc cgcatccagg gatgggtcaa cggtgaagag aggtttcagg atttcccgat
    17881 gaagagagtt acctcaaaaa ggtagaatcc atttctccct ttatcttttt acaactttct
    17941 taaaaaccgg aaaaccggaa attttacatt cgaataaata caatcccgaa ggaaattccg
    18001 tcaggtcata aaccatttgg cccgatccaa tcctttcaga cctgacaagg actccctgca
    18061 tgttaaaaat attcagtaaa tattctccgg aaaagtcatt aacatccaca tttacaattc
    18121 catccgtcgg attcggataa atctttacca gatgttcgaa tccccgttcc ccgatataaa
    18181 acccttctcc tacatgataa gtgtaaaaaa tcgtccggcc aaattcggaa ttgaaagatt
    18241 tgatcatatg cccctccaga tcgtaaatcc ggaaataacc gcttccctgc tccggatagg
    18301 cccagtaact caaaccaatg tcctcaatat cggtcagctc aatggtatag cagccataag
    18361 gcagatccag cgtgtcgcgg taggtagtgg tattttcgag gttatccttc cggagaatga
    18421 cattcccgga catatccctg gcctccaggc taaaacggta ggcctgctta ttggtgcgta
    18481 gttcaagtac gatgggaatg tcgatcaggt cgggcatatt gaacttcgac cggtatgtat
    18541 cattagccgt gtattgatca tccgttccgt tcggctcgct gaccgtcact gtgaacgtat
    18601 ggttttcgtc gccgatccag aagaggtcgc cggaaacggg cagatcgacc gtgtcgatgg
    18661 tatgaggctg gaggctgcca ctccaatcaa acgtttcctg cgggccgccg gagacaccgt
    18721 aactgaactt cgccgaagtc agcggggctg agccattgtt ccgcaacagg gcttttaaac
    18781 cgtagcacac cgggttgacc cgtgaatagt agcccacgtt gctcggtgtg atcacattgt
    18841 acacctcggc atccacatta aagtgattct cgccatactg gacaagatcc atattggtca
    18901 catagtttcc ccatcccatc cccaggttgt cttccggaac aggcgtaatg tcataatcga
    18961 tcgtaacctg gctgccggta atgaaatccg taatctcaaa atcatggtct ttcaccaaat
    19021 cgcccgggca ccatccttcc cgtgcacccg accaggtgcc cccctgcgga aataccgggt
    19081 taagcgaaca atcgtgatac tgaaagatat gccaggcgcc tgcctgctgg ccatttactc
    19141 tcaggtagtg ctcattgtct ttccattcgc agcagtgcgg gtactcaccg gtattgctgt
    19201 tgtggccgtg gcccgtcagc cgtgtcttca ccctgaactg gctggcgtcg ggacgcaaag
    19261 gaagggtgac ggcgctcatc tgcaggtcat catccaggtc tttgtagtag aacgacctca
    19321 gaccgcccca gatccggtcg atctgcttca cctccctggg tggagtgcct ttgatgagca
    19381 taaatttcag gtcgatcagt tcctgctggt tgccggcgct cagatcgacc attccctgca
    19441 acaacggggc atagtctgtc acatcataga cccaggtaaa tccgttatca ccaagggtaa
    19501 gcccgatccc gtaaggggtg ataaaacgtc ctatctcaaa ttcttccagc acttcgaaag
    19561 gctctccgta atacgggatc atctctttac ggatcacctc ttccggcgct gtccagacgg
    19621 aatcaaccac catccagttc tcatacacag gtttgaagcc cgctacccat ttttcaatcg
    19681 tatctgtagg aaaggcagga ttatccggat cactgaacag cacgatctgg agcggcgcat
    19741 ccatcagcga gtccatgaca gtcacttcgc tgacatcaac ggttcctgat acaatcctct
    19801 cgaaaatgat gttcggacgg ttgttatcct gctcaaatcc catgtaaacc tccggccccc
    19861 ggacgggata aaaacccggc cggccaaacc cttcggcatg atggccgttt attgatctgt
    19921 cgtggagaat atttcccgat ccttcatcga aatcataata aaaaacaaga ttcgaatcgg
    19981 ctgccggatc ggcgatccct ttcctacagt gcgatttgat ggtcgccggg tccagggcgg
    20041 ttttccagag gcggaattca tccaggagca catctgccgc tttgtcgccg ttccagttgc
    20101 tcctcccgat atagttgatg cttcgttcca ggttcggagg ttgctgcagc aggcccagct
    20161 tcaccaggtt tccgttaatg taaacccagg cgatatgagc cgtcagcctg acggccaggt
    20221 gggtccattc ataaagtggc gttgggttat ccagctcgaa cgaccggttc aggccatcca
    20281 tgaaaacatg aaatgaaagc ctcccgctgg ttcccgttga aagggcaacg atcacattct
    20341 gcctgtcggg cccgttgccg aaatcgaaaa tacggctcca gttgttgtta tttcgcttga
    20401 aaaaccaggt ctcgaaggta aaatcgctat tgaagtaagt gcctggctgc agttttaaga
    20461 aatcagagac accgtcgagg tccagcccat aattatcacc ggccgatgat attccgcaat
    20521 taaaaccggg gtcctccgcc cgggccatca cttctccgct actccccgaa ccggtataac
    20581 agaactccac gacaatgtct gagacgccat cccagtcaaa cggccggaag aaactgaaat
    20641 cattccagcc ggttgaaaag ctgatccggt tttggaatac agtatccagg cctgcgatga
    20701 gggtatccgg tgtcagttca tccaggctga cctgtttcat ccggatcata aaatctcccg
    20761 atccgtttga accttccgcc aggttaagct ttagacctgt taccggacct gcttccagac
    20821 cctgggctga gagttccgtt gatttccaca ggtactgtgc acgaccggcc cggtattctg
    20881 cagggatgac tttatcgttt tctaccgccg gattacctat tgtggcctgt gtaaaagaca
    20941 gcgtatccgg aaaaacaaca tggtaatggc ggttcagaaa atagttatac gtcggtgaag
    21001 atgtcagcaa cagcgagtcg ggagccgttc cttccaccag ggtgaaattc ggatgatggt
    21061 aaagggttga gtcataggca ccggtatgcc tgtaaacggt gttgtatgtc agataatccc
    21121 attcaccgca ggcatactgg tcgtgggtgg tggcaaagtc gcatttcagc gtatgaaaca
    21181 tgaggatctt ccgaaagctc tccccttcgg ggaattgcca ggtaccgcgg cgggttgtga
    21241 tcgaatcgaa tgtaaatgtc tgaacgataa gggtatcctg cgcagcagaa gtaattacca
    21301 taagcgtcag gatgaaaacc ggcaggatct ttctcataga tggatttctg aaaatatttc
    21361 ccgtaaaagt aataataacg ggaaatgtat ttttccggcg gatattttga aatctttcga
    21421 ccgtcgggta aatccgtcgg tgtaagatta tctcccgggg gaaaatattt tataaaatga
    21481 taaacggtgc gaaactcttc ttaatattgt aatcacggat aattaaacct ccggatgatt
    21541 caattattaa caaaaccaaa aaaaactcat gaaaaaacta ttattgattg tggttctggt
    21601 accggcacta ttcctgacat cgtgtttcaa ggaaaaagcc ggggagcttg gcggagacca
    21661 gtcgccaatg ggtgaagtcg gcgtcacggt ttccagttca tccatggaaa ttgccggtgt
    21721 cagtaatttc actgctacgg taacggcctt gaaggacggt gtttcatcgt acacagcaac
    21781 ggccacggta accaatccgc tgctgaaaaa tattgcggcc aattaccccg gagtggaggt
    21841 taacggcgac caggtaagca ttacaaacgt gaaaatccag cagacgaaag aagggatcaa
    21901 aagcatggaa ggtcccggcg ccggcgttct ggtcaagtat gattcaaagg tcggcgatac
    21961 ctaccctatc gatggcaccg acagggagag aaaagtggtc agcaaatcga ccgacgatga
    22021 ttattactac ggattcttcc tgatcaaaac catccaggtc gagatggatc agtcgcacgt
    22081 gaaaagctca ggtatcacaa aatatacata tatcgctaac cacaagtttg gtttggttgg
    22141 agtgaaggta gattttgacg accaaaccag cgttacgttc ccgatctaca cctctgccga
    22201 aaattaaaca ggtaaatccg gtctgactga tatttaactg atgggatcgt ttttattgcc
    22261 ttaccgctgg aaaatcgcag gtattgtgat ggcaattgcc ggcatggccg gtgctgccgt
    22321 ttacggtttt ttcgattttc gctttaaggt acctgttttt gctgtcgttt ccattttcgt
    22381 agaaccgaaa ttctttaccg tcttccagac caatttttcg gaagaggttg ttttattttt
    22441 tctgattaca ggattaaatc tgatcatctt ttcaaaagaa aaagaagagg gggaagattt
    22501 ggaaaggata cggttgaaag cattcgccag ggctatgatc accaatgccg cggttcagct
    22561 ttttgccgtt ttgtttgttt atggcgccgg ttttgctgcc gtaatggtat tcaacctatt
    22621 gtctgtcaat attttgtatt tgattttctt ttacaggatg agatggagtc tcttaaaaaa
    22681 ttgagactcc atctcaaatc aaatctattt atctatattt gtccgatttt taaaaacggg
    22741 cttcaatgaa cagaatcatc ttcatttctt tactgctgat cacagcaatc atcccaacat
    22801 gggcgcaaac tccgggcaca ttgaccgtgt cggtttcaac cagcgcggcc ggagggaatt
    22861 atgcaccgaa aaatattgtt gccatctgga ttgaagacaa ccagggtaat tttgtgaaaa
    22921 cgctgctggc ttatgcacag acgcggaaaa cccatctcaa cacctgggaa gcctcaacaa
    22981 ccgctgccgg cagtccgtac aacaccgtcg acgctattac cggcgccaca aaaaccagcc
    23041 atgccacccg cacctgttcc tgggatggta ccgatatcaa cgggaatatt gtatccgatg
    23101 gaacctacaa attaaggatg gagctgactg ataagaacgg caccgggaat ttctccactt
    23161 tcacctttga aaaagataca ctccaggtaa atctaacccc ggccaatgta ccgagcttct
    23221 cgtcaatcgt cattaactgg gaaccggtaa ttactgctat cggtgaaccg gaaacagccg
    23281 gcagcatcac tatttatccc aatccgtcat cgggtgtcat caatcttacc ggaaaatatg
    23341 atgaagtaat agttttcggg acatccggca ggcaaatcgt gaaatccgca ggtaataaga
    23401 ttgatcttac ggcatcaccc gatgggcttt attttgtgca cgtgagaacc ggagtggaaa
    23461 cagaaatttt caaagtcgtg aaaacaaatg acctcgaaaa ataaaccgtc gccctccagc
    23521 gactggaaat cacgccttgg tgtagtatat tccaccaatc ccgattattc atacgcaacc
    23581 ggtgagagcg ctgaacccgg caccctcccc ccccaacagc aaaacctccg ggtgagtctc
    23641 gaccggaagc agcgcaaagg caaatcggtc accctgatcc agggatttat cggcagggat
    23701 gaagacctgg agctactggc gaagcagttg aaatcgaaat tcggagtcgg aggaagcgcg
    23761 aaagagggag agatcatcat ccagggcgat ttcgtggtaa agacaattga attcctgcaa
    23821 aaccttcatt acaaggtcaa acggtcgggc ggctgaagca taaataatca tattgaaatg
    23881 ctgccggtat tcagtcaggt ttgtcaataa tattcttatc ttgcattgcc tttttaccaa
    23941 aaacatcccg ccatgacaaa gtcttatctg tattttttga tattgatgat tccggccggt
    24001 ctttttgccc aggtttctcc cgactggtca atgatttata atggcaatga cgattatttc
    24061 gataacggag tcgcaattgc cctagatcct tccggaaatg tgtttgtggg aggaacatca
    24121 gtatcgatca ccaatggagc gcccgactat gtcgttttaa aatacacatc tgccggtgag
    24181 ttcaaatggt cctatgaata tgacggtacg ggagcttatg aagatttcct ggccgcaatc
    24241 accacagata gtgaaggaaa tgtttacgct accggtggga gccatgaagg gtcctcaaga
    24301 agggattatg tcaccctgaa gattgatgga tcaggggcat ttgcctggaa aagtatctat
    24361 aaaggactgg atgtgaataa tgatgatgct gctgcggata tagttgtgga tgagaatgga
    24421 tacgtgtatg ttaccggtgc cagtgccgga tattccagtt caatgtggac agattttgct
    24481 acattgaagt tgactcccgg cggcgacacg gtctggaccc ggcgcctggg aggacccggc
    24541 atgccaaacg accgggctac ggcaatagca gtagataatt tcggttatgt ctatgttacg
    24601 ggttattttg aaggggatgt cagcacaaga tataattatg caacggcaaa atataaaccc
    24661 aatggtgata cagcatgggt aagggtttat aacggcaccg ggtctgatga agacagggct
    24721 tatgccattg cagtggatga aaataggaat gtttatgtga ccggctcaag ctggggttta
    24781 aatgctgatg attacgcgac agtcaaatac gattccaatg gtgttcagaa atgggtcgcc
    24841 cgatacaatg ggccggccgg ttcatatgac aaagcttggg atttgaaact cgatgatgag
    24901 ggcaatgttt atgtaacagg gcaaagcggc ggaacaagcg gtaattatga ttactgcacg
    24961 ataaggtata atccggatgg aggtgaagac tgggttgcca ggtataacgg tccggataac
    25021 ctggacgacg tggccacatc agttgccatc gatccttcag gaaacatctt tgtaacaggt
    25081 tattcttata atcctccctc gccatatatc agcacaaccg atatggttct gataaaatat
    25141 aatcccgaag ggctggaaga atgggtgagc cggttcgacg gcccgggcaa taattacgac
    25201 caggggtatg ccattgctgc cgatccttcc ggagatgttt tcgttaccgg ttcaacagaa
    25261 tattacggtc aacaaaattc cagggatatt ttcacggtaa aatacgccgg cccttctctg
    25321 gtggaagatt tttcttccgg taattccatt aacctttatc caaatccggc cgatcatcaa
    25381 ctgactgttc aggttccgtg cggggctgcg ctgatttccg tttgcgacct aacgggaagg
    25441 cagattatcg tgcatgaatt gaaagtatcg gaaaaagacg aacaaacatt caatatttcg
    25501 ggcataccct ccggggcata tattgtaaaa gtaacgacga cactgggtat atcagaaaag
    25561 aagctcatag tatattagat ttgagattat ggtgaaaatg acagcggtct accattcggc
    25621 agatgaatat attacatgtc agccggaaca attccgggcc aacctggccc tgatccggca
    25681 aacaatccgg gaagctgttc cggatgcaac ggaagccatc agttaccaga tgcctgcatt
    25741 caaatttcat ggcattctgt gcttttatgc agttttcaag gatcattaca gtctttttgt
    25801 atctcctgaa attcacaagg cctttgttga ggagctgaaa agctataaaa cgaccaagtc
    25861 ggccatacac tttccatttg accggccggt tccggttgaa ttaattgttc aggtggtaag
    25921 gtttgcggcg attacgaatc tggaaaaggc cgaagccagg ttaaagaagc ggaagagatg
    25981 aaggttattt tgagactcca tctcggttaa tccagatgga gtctcgcttg attgagactc
    26041 catctcatga ttcgagatgg agtctcaatt ttcctacctc aattattaat aattttaccg
    26101 gcaaaaattg cccgatatgc tgacattgcc cgattattca gacattatta aagcccagat
    26161 gaggctgaaa ggggttgtga aaaacacccc actggagcct tccaaatcat tctccgccat
    26221 gtcgggcgcc cacatctacc tgaaactgga aaatttccag tcgacgggct cttttaaggt
    26281 cagaggggca tataataaaa tcagttcgtt ggatcccggg gaactggcca acggcgttgt
    26341 gtgcgcttcg gcaggcaatc atgcacaggg cgttgctttt gccgcacggg ctctcggtct
    26401 gagagctacg gtttttatgc cggtgttcac gccgcctctg aaagtgatcg ccaccagaag
    26461 ctacggtgct gacgttaagc tggaaggaga gacattcgac gatgcactgt cagcggcaaa
    26521 gaaattccag gaatcatcgg gcgctgtctt tatccatgcc tttgatgatc ccttgatcat
    26581 tgccggccag gggacgatcg ggctggaact gttccaggcg ctggaaacca tacaggatgt
    26641 ggtggttccg gtcggtggtg gcggattgat cgccggaatt gccattgcat tgaagaagac
    26701 caatcccgat atcaggatca ttggcgtgga agctgagggc gcagcatcca tgcagacatc
    26761 gctcagggat ggaaaattaa cccgactaac cagcatgtcg accattgccg acggtattgc
    26821 ggtgaaaacg cctggtgagc ttacttttgc agcagcgaaa caatttgtag acgagattgt
    26881 gacggtttca gacgcggaga tcgcccacgc ggcttatctg ctcctgcaaa gggcgaaact
    26941 gctggctgag ccggcagggg tagcctcaat ggctgctgtc cttcataaga aactggattt
    27001 caagaaccgt caggtcgtga ctgtcatcag cgggggaaac atcaacatgg gtttgcttga
    27061 gcagatcctt gagaagggga tgatggctga aggcctcaga gcgcggatcg ccgttttgat
    27121 cccggatatg cccggcgaat tgaaatcggt gattaccatt ctggaaaacc tgtttgccaa
    27181 tattaatgaa atcagccatg agcgttcgat cactacggtt ccggtaggcc atgtgctggt
    27241 aaccatcacc tttaaccttc agcagattga gcagctcgac atggtttgca aagcgctgga
    27301 ggggaagatg aagaagtttg aggtgctgcg atagggatgt tctgatgaga ctccatctca
    27361 aattcgagac tccatctcga ttgcccagat ggagtctcaa tgtatcgaga ctccatctca
    27421 aattacaaat cccattcttt ttgtacattt aatccctcaa agaacaactc ctttatgtcg
    27481 ttgaaaataa agctccctgc ccttcactgg caaatcctga tcgcactggc attggcagtc
    27541 ctcttcggaa tatatttttc cgaatacgta aaatatgtca gctggatggg aacgatcttt
    27601 ctcagggccc tgcaaatgat cattatgccg ctggtaatca gctcgatgat ctgcggagtg
    27661 gctaacatag gcgacgccca gaacctgggc cggattggat taaagacaat gctgttttat
    27721 gtcatcacaa cagtgatagc tatcttaacc gggcttgtgc tggttaattt ctttcagccg
    27781 ggtgtcggcg ccgaccttaa cctgatcgaa aaagcggaga tccctgcagc ctccggttca
    27841 ttagggcaaa tcctgatcga tatcgtaccg gaaaatgtct tcgccgtcat gtcggtcaac
    27901 cggcaattgc tttcggtaat cctgttcgcc ctgctttttg gattttttat catcaaagta
    27961 tccgaaaaac caagaaaaca gcttaccgac tttttcaatg ccgtgtttga agtgatgatg
    28021 aagatcactt ttttcattat taaattcacc cctctgggaa ttttcgggct ggttgcagaa
    28081 aagatcgccc cgcacaaaga tgtcgcagcg ctttttgaaa gcctcggaat atatatggta
    28141 gttgttttat tggggttatt cattcatgcc gttgtattta tgccgttgat catccgattt
    28201 atcggccgta ttaaccctct ccggcatttc aatgccgtaa aaacggctct gttcactgct
    28261 ttctcaacat cttcctcaaa tgccacactt ccactgacaa tggaatcgat tgaagtgaat
    28321 gccggggtgt ccaataaaat cacgagtttc actcttccat tgggggcaac cataaatatg
    28381 aacggaactg cactttacga atgtgttgcg gtcatcttca tcgcccaggc ttacggcatc
    28441 ccgctcagct tcggacaaca ggttattgtc gttgctactt cgctgctggc tgccattggt
    28501 gcagccggtg tgcccatggc agggctggtg atgatgtcga tcatattatc agctgccggt
    28561 ctgccacttg agggaatcgg
//
LOCUS       DAOPNH010000007        25403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_1741_length_25403_cov_6.491952, whole genome shotgun sequence.
ACCESSION   DAOPNH010000007 DAOPNH010000000
VERSION     DAOPNH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_25403_cov_6.491952"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKZ74_01000"
     CDS             complement(<1..1003)
                     /locus_tag="PKZ74_01000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM91354.1"
                     /translation="MLFNSISFAVFLPVVFLLYWFVARKSLRGQNVLLLIASYFFYGS
                     WDYRFLFLLIFSTFLDYFTGIKIHEAETRKRKKIWLWLSITINLGFLGVFKYYNFFAA
                     SFAGALSLVGFHADYFTLQVILPVGISFYTFHGLSYVLDLYNDRIKPERDFINYAVFV
                     SFFPLLVAGPIERATHLLPQIIRKRDFDYHQAMDGLRQMLWGFFKKMVIADNCAIYVN
                     DIFSHSSGYSGSTLVLGTMFFAFQVYGDFSGYSDIALGTARLFGINLLRNFAFPFFAR
                     DIAEFWRRWHISLTTWFRDYLYIPLGGNRGGKWMSIRNMFIILQISCSIQGIYLLPPE
                     "
     gene            complement(1027..1938)
                     /locus_tag="PKZ74_01005"
     CDS             complement(1027..1938)
                     /locus_tag="PKZ74_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91355.1"
                     /translation="MKKYLINTAIFILAFLILVIALALASSRLVYKRAFRNWEVEGNL
                     LVMKSNHDYNLLMMGISHARNFSRHKNHLRMEKILHQEIINIGIGEGKCGAIDQHFFL
                     EYFYYKRNSADRIFYVMSPTLLNKGFINRASNTFEFEPFRLDFFIKYLGYKSDNKYER
                     LFYYVKSKFARKWYRLQPRSLDEKKSALKEIDSTKIKEGLKLLLVDGERMDEFQRNCL
                     VVEETIKLAKEHNTEVIFFIPPALFGKWEGHYETMDFLEEMYKKYGTRYYDFSESVLI
                     PEYYYDHHHLNSKGIEYFAENYLKGIL"
     gene            complement(1942..3381)
                     /locus_tag="PKZ74_01010"
     CDS             complement(1942..3381)
                     /locus_tag="PKZ74_01010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM91356.1"
                     /translation="MLFNSIEFAIFLPVVFLLYWFATGKSLRWQNILLLIASYVFYGS
                     WDYRFLFLLMFSTFLDYFTGIKIHEAKPGRMKTFWLWLSISINLGFLGVFKYFNFFAS
                     SFAELLGMFGIQAGFVTLNVILPVGISFYTFHGLSYVLDLYNDRIKPERNYVNYAVFV
                     SFFPLLVAGPIERATHLLPQIIKKRVFNYSQAVDGMQQIIWGLFKKVVVADNCAPIVN
                     KIFENPGAFSGSELLIGVVLFAFQIYCDFSGYSDIALGTARLFGITLLRNFAFPYFAR
                     DIAEFWRRWHISLTTWFRDYLYIPLGGSRGGKWMSVRNTFIVFLVSGFWHGANWTFIV
                     WGFLNALYFMPLLLLNKNRQNLEIAAKGKWLPSVRDLAGMVVTFSLTLIAWVFFRADS
                     VSGALIFIQDIFSASLFTAPKIRTHYIWVFILILLVFEWIFREKQYGLQFNEVRLHRS
                     LKWSFMLALALVIFIFGNFNKTEFIYFAF"
     gene            complement(3387..5237)
                     /locus_tag="PKZ74_01015"
     CDS             complement(3387..5237)
                     /locus_tag="PKZ74_01015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM91357.1"
                     /translation="MSFLTEKYRPWTPKWFVQRVVVVLKEGRMPQLGHLFGLKPFNPR
                     EPIPANKYNRLKKAPVELSMMEIHIPEEIKKANRINSYFDKIYLLNLKHRSDKRLKSI
                     FQLKQMGINAWIVDGINGNEEPHKSEYEKYLSIPLGEPDAHPLEKKYNRKMISSPGAW
                     GVLKSKKLIFQDAIQNNYNRILILQDDLFFIEDFHRKFEEFIRAIDDDWKIVGLGATQ
                     HAWLFPGSLFYDDLSITEYDPGKKFYYPLYTDGAFALGYDQSVFPEIIAEIDKMNCSF
                     DSGPVRAIYHKYRKKCYICQPNLVIADVRTSDIRGGRDQVEFSKRMKWNLPEYDLKKY
                     QDDLVSVIMPAYNAGRTIEKSIRSVLLQTYQNLEILVVDDGSTDNTAGIVKRIAKEDD
                     RVIFIRNEQNRGCYFARNDALRQSKGKYIAIQDADDISLKDRIEKQLIPLVSGNAFVT
                     FSLFLRSRCTPEELDLLDQNAMLELAESRRKQVDGKYDYFDKYNVALATSVYRRDLFE
                     RYGLFWESRFGSDNEFLERIFFHELGLSFDEDSGNAHSLITKMRQMPGAFQLVEELLY
                     ISPQMENSNLSVTYTIQGDERKEFTRVCRDKLKGIGNYQYPEFNNTGRPE"
     gene            complement(5234..7126)
                     /locus_tag="PKZ74_01020"
     CDS             complement(5234..7126)
                     /locus_tag="PKZ74_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM91358.1"
                     /translation="MTHRKMAFLRKTYRPWTILWVIQRFIVVMKERRLPQVRMVDLMY
                     DFRLTRKIRGDVRNRTWNTGLADPDVAGGLFNFVPTLEYKPFIINDDVDENELSVINH
                     IFDRIYVINLGRRLDRRVEMIMKLRKYCIKAEIFEAGNGYSKENSEAFAAYYDLPLAA
                     EGCHPLEKELKKKMIASPGSWGYLLTWKYLLNDAKSKGFARILVFDDDVLFHKDFEKK
                     TGESLKSIPGNWKLLYLGATQHDWSVPYNVNYPDKVGEKDFGVHPFYYPVKTDGSFAV
                     GVDCSVFDEILSEIEPMNCALDSGPLRAIQQRYPGQCFVVKPNLVVADVGQSDIGVER
                     DQGQMARKLRWQMDLYDYPFRKELVSVIMPAYNAEKTIEKSIRSILMQTYRELEMIVA
                     DDGSTDSTVAIVDRLAKNDSRVKLVRLDSNQGCYPARNAALRVAKGRYIAIQDSDDIS
                     LATRIETQLIPLLTGRALFTLTRILRGRCSIDELDIGNQREMAQLVLDRRMESASGLY
                     EYRDRPVIGFMTSMFRRELFEELGLFWEHRFGADAEFLERVLFQKAGILLSKKDGTIH
                     SFLMDRDAIPGIYERIDKVLLISADMTGDNITNRHSQKEKDAFEGLWRQRLRGEYQYN
                     YPKFNP"
     gene            complement(7123..7893)
                     /locus_tag="PKZ74_01025"
     CDS             complement(7123..7893)
                     /locus_tag="PKZ74_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM91359.1"
                     /translation="MGKPIIIDIKNVSKRYKLYRNKKDQALQALFPFLPRKHTDFYAI
                     KDFSLTVNKGDILGIMGRNGSGKSTLMKLVAGVIQPTKGTITVKGNLVPLLELGSGFH
                     REFTGYENIYFYTAVLGYPKKKVIEKTAEIIEFADIGEFIHQPLKNYSSGMKTRLAFS
                     VSVNIDPDILILDEVLSVGDAAFKTKSYDKIKEFFNQKKTILFVSHSPESIKALCTRA
                     ILIDKGKLVIDGDPAEVVKKYSSIYGGKKVSEKIMDLE"
     gene            complement(7906..8733)
                     /locus_tag="PKZ74_01030"
     CDS             complement(7906..8733)
                     /locus_tag="PKZ74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91360.1"
                     /translation="MKLIYLIKRFFIEGADFLKQIYLNKFIIWELTKRDFKTSYVSNI
                     LGLSWAILEPLAMMFVLWFVFTFIRTGQKTSVPFALFLLAGLIAYDLFNKALNSATRG
                     ISNYGFLINKVHFRSAIIPLVKISSELIMHGIILVIVSTILILSGIPVTIYWLQVFYY
                     LFATVFLLVGLTWLTSSVSLFFPDIKYIITIVMRVLFFFTPIFWEMKNIPENMVLIFR
                     LNPLYYIVKGYRDSFLYGIPFWSNWTDTAYFWGLAGAFFIIGVLVFKRLRPYFAEMV"
     gene            complement(8753..9709)
                     /locus_tag="PKZ74_01035"
     CDS             complement(8753..9709)
                     /locus_tag="PKZ74_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPM91361.1"
                     /translation="MNKDSKIYIAGHNGMVGSALHRNLEAKGYRNFIFRTFDELDLTR
                     QDDVEAFFEAEEPEYVFDAAAKVGGIVANDTFRAQFIYENLMIQNNLIHSSYKNGVKK
                     FLFLGSSCIYPKLAPQPLKEEYLLTGSLEPTNEPYAIAKIAGIKMCENYYRQYRSNFI
                     SVMPTNLYGPNDNYDLQNSHVIPALLRKFHDAMISNAPSVTLWGTGQPLREFMHVDDM
                     ARACILVMEKLDAPKLYDDLGLTHINIGVGEDITILELGRLIAKITGYTGKILFDTSK
                     PDGTPRKIMDNSRLRKLGFKPRISLEEGLAQTYKMLSLSVQH"
     gene            complement(9702..10862)
                     /gene="gmd"
                     /locus_tag="PKZ74_01040"
     CDS             complement(9702..10862)
                     /gene="gmd"
                     /locus_tag="PKZ74_01040"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPM91362.1"
                     /translation="MPKTALITGITGQDGAYLAEYLLKKGYIVHGIKRRSSLINTGRI
                     DHLYEDPHVENQKFILHYGDLTDSTNLIRIIQDVQPDEIYNLGAQSHVRVSFETPEYT
                     ANSDALGALRLLEAIRLLGLANKTRFYQASTSELYGLAQEIPQTEKTPFYPRSPYGAA
                     KLYAYWITVNYREAYNIFACNGILFNHESPIRGETFVTRKITRAVARIAMGLQDKLYL
                     GNLDARRDWGHAKDFVKAMHLMLQQDTPGDYVIATGKTTSVREFVKKAFAYIGAEIEF
                     RGEGVEEKGYLNKMVDGPLSMVDGRWKKIENRPSKIENRKSVVEIDPRYFRPTEVDIL
                     QGDASKAKAELGWEPELSIGDLIGEMMEADLELMRKEQFLREGGYEIMNYYE"
     gene            10972..12201
                     /locus_tag="PKZ74_01045"
     CDS             10972..12201
                     /locus_tag="PKZ74_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM91363.1"
                     /translation="MERSIPIVVVGYNRPKALKRLLNSLLKADYPCEVELIISIDGGG
                     DEENKKIASSFSWPFGKKQLIFHEQNIGLRKHVLSCGDLVKDYDGVIVLEDDLYVSPG
                     FYEYTLKAFDFYKDDDKLAGISLYTHAYNETAQFPFRPLADGSDVFFLQYAPSLGQFW
                     SREGWRRFRDWYDTLVPGEPIPVNLPPNIKLWPETSWKKYFIAYILRHDLYFAYPRSS
                     FTTHFSDEGTNMRVKENLFQVPLWLDKKVFVFRAFGDSNAVYDVFCEMKGEKLRQRVP
                     ALAGYDFGVDLYGMKSKEDTDGEYFLSQRRCKNPVMQFGREMRPHEMNITEGIEGEYF
                     SLGKKEDFEEKPFMVRLLKCHEKQELAYWYPIREYHFYKNRLLTTGKTQNFFLNPVFL
                     FRKFMVMARYAYRYFRI"
     gene            complement(12346..13191)
                     /locus_tag="PKZ74_01050"
     CDS             complement(12346..13191)
                     /locus_tag="PKZ74_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91364.1"
                     /translation="MKHLLTWLGRHKAIAILLAVITYFSIVTFHDEITQVAIRVRNAV
                     GRDHYNAYLAWFFIALLLAVLAWMAWHIGRSRRIWLNTSLVVVMAGLMIAAFNYLMVY
                     NIEAIHFVEYFLVAFLLLPVARSYGGTVFWVTLLGVLDELFQYFFLVPEFGYFDFNDN
                     VLNLLGAGTAAVTVFIVNNGVIKMKYLRWYRSPAVITGLVMLASFILLMVTGLMTADP
                     MPGGDNLFSLNREAMPDEFWKEAYPGRSFHILRPWEGMTLIYVLFAGFFMLDWLVIRV
                     SNPEF"
     gene            13531..14310
                     /locus_tag="PKZ74_01055"
     CDS             13531..14310
                     /locus_tag="PKZ74_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91365.1"
                     /translation="MTKISIITPSFNQLHFLKETVDSIHNQQGDFALEHIIVDGGSTD
                     GTVEFLRSLGDCVRWVSEPDRGQSDAVNKGMQVATGDIIGWLNSDDLYMPGALQTVVR
                     HFHANPDCQWAYGRCIIIDKEGVERWKYITRYKNIRLRKFTLDKLYRENFISQPAVFF
                     RKDLWNQAGGLDLSLHYAIDYDLWLRFALRSPALVIDSDLAAFRRHGASKSETGFRDQ
                     FYEQYAVAKRYHPSGFNRLIHRFNIFKIITSYRIIGLFFRF"
     gene            complement(14224..14676)
                     /locus_tag="PKZ74_01060"
     CDS             complement(14224..14676)
                     /locus_tag="PKZ74_01060"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HPM91366.1"
                     /translation="MNESLGSIGKNIQTTQVIAILVSVGLFLFILYLVRKKKIKEEYS
                     LLWLFSSVVFIVFSIWREGLEYFAGLMGIAYPPAALFLILLLAIFLILIEFSINISKL
                     SEKNKVLAQEMALLQNELEKLKAEKQADDPVRGDDLEDVEPVDKPVES"
     gene            complement(14669..15385)
                     /locus_tag="PKZ74_01065"
     CDS             complement(14669..15385)
                     /locus_tag="PKZ74_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91367.1"
                     /translation="MKKILVIVPAFNESENIREVISKLHSENAGWDILVINDASEDKT
                     SEIARATGQAEVIDLPFNLGIGGCVQTGFRYARRNGYDFALQFDGDGQHSADEIHKLL
                     ELVRSGEADVAIGSRFRQKHDGFKSSSSRRMGIRIFEWFSFILIRQRITDHTSGFRAY
                     NRKALKFLADYYPSDYPEPEVVILLGRNGFTIRETFTQMHERKGGVSSIPITKGPYYM
                     IKVMLSMFMSAIRSKVYRYE"
     gene            complement(15410..17443)
                     /locus_tag="PKZ74_01070"
     CDS             complement(15410..17443)
                     /locus_tag="PKZ74_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91368.1"
                     /translation="MIEKQPLIKKYAVILIAIVGILAYANSFNNSFQFDDGFHIIDGQ
                     KIKKMENVTRLSHWKAVGNRPLSFFTLAVDYKLADKDPSGRPAVQGFHVSNLIFHILA
                     GFMAFLLALEIMSLNIFRNNKIIQDYKVLIALSAGFIFVAHPIQTQSVTYIVQRMAVL
                     AGLFYLWSIVLYIRGRRAHLDPSNKKAWKPWAFYAGAFLAGFFGFLSKQNAITFPIAF
                     LLVELFFIRDKEMRIDKKFLIAASSIIGIIIVFGLIINGLPREFDRISRSDYLVTQFR
                     VLVKYWQLLILPVNQHLDYYWPVSQSLWGVKELVSLGVLLLTVALGVWLFMKKWYIAS
                     FGIFWFYLTLSLESSIIPIRDLIFEHRLYLATFGFGFVISYLAFYFLAPKNIKYPIIV
                     LSILALAYIGASLNRNSVWKNPYTLWTDSIKKSPKRERAHYWLATYYMEVKDNEKAME
                     SYNTSIECNPGFPLAYNGRANLKKELGKNKEAIEDYNKAIKLDPNYITAYYNRGITYA
                     AMNKFDKAIKDYNKSIQLGNNASSVYYNRANSKRKNKDMKGALEDYNTSIKIDPNFPL
                     CYFNRGLTRAQMGDHVEAIKDIDRAIKMDPRNHLFYNGKGVSQISLQQYKEAIGNFDK
                     CIQLNPNFGQAYYNRGYAKQKGFNDKDGACKDWEISVAKGYKAARSYIQQYCK"
     gene            complement(17515..19785)
                     /locus_tag="PKZ74_01075"
     CDS             complement(17515..19785)
                     /locus_tag="PKZ74_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91369.1"
                     /translation="MLIPAKSQVITVKQDGTGDFTVIQEAIDASVNGDTIIVYPGTYY
                     ENLDLTDKGIVLASTWIYAHEDSLITHTVIDGNHTGSCIQSLSGNLWTSIIGLTIQNG
                     FGTNHSTLFPWAYGSGGGILLENSKMNVYQCRITNNYGQVGGGIYSTESSLELSGNTI
                     SNNWAQRAGGGLCIGFSLVNLDSINLNNIYLNYSSSGSDIAIYYNDTPSKIWLDTATV
                     LNPDQYYIGKFSNRAVHIPGPPLSVLHGKIDQVNADLFVSPEGNNSNSGLTINDPLKT
                     ISYALLKIATDSINIKTIHVAEGVYSNILNGEHTPIQLKNYVNLIGQGMENTIIDCED
                     KYEGARFAFGQEYSKIKNITIRNGNGYFNLGDGGISSGYSRKLVLDSVAVKETTGFHY
                     TGFYSDSDDTIIMKDCVFQDCYGYKTAGLFINYDQPPRYSEFISCRFSGNRPDTSVYR
                     RHTSLTLSGLSYTNDLNKGKIINCLFADNTDSLFWSGYGGSVALSIISSQSDIINSTF
                     ANNRTVNNPRGGAIGIMDGSVVNLYNCLLYDNYGCQAYLTDFQTVNRDTININFSLVQ
                     DGQEGINKYGTNDLLVWGNGNLDANPLFFGSGEHPYSIDFGSLCIDAGTLDLPPGITL
                     PDFDIAGNPRVYGEAVDMGAYEYGPWVGVPAAPGSRFQVPGSKLLNVNPNPFIFGTYI
                     SYELKSAGRLNISIFNSSGMLVKTLVNFNGGTEEKGEIYWDGAGPGGTALPAGIYFVR
                     LTIDGKETETVKVVKE"
     gene            complement(19863..23654)
                     /locus_tag="PKZ74_01080"
     CDS             complement(19863..23654)
                     /locus_tag="PKZ74_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91370.1"
                     /translation="MKTILVKILWCLCLLLPAYPSFSQNDPFLGTPEIIYLPKNNLGV
                     MTEYTLEVDDYINGIHIHKLYAYGMQGVLIYNLNLTNWEEVPVKIEFTNEVSGFYDRP
                     FTDFLELPARVPMIYISDENCPGQIFAVGPDLRLWYIDVTDDTPHQCQIPSGLIGYKL
                     SSAKLVYDNDYSRIVWMIRGMANNTGNSKSWLCTVNLSSTNYPVQIIQCLDYIITDFA
                     INDIVSNNIIYVGVFTGDDTGYWALLDRDDFEPVPGKQVPVNFRPGLIRYVHTNDVHQ
                     VYCLPGANPPSTTTSVKIYEGDISSHDPLTMLSPAAGYTCAGFNDHHNHLFLGAYAEP
                     AVTEFNDVYVFQFQNNTSPPVLKCELNTYDPPRVRQNVPLAIESVSNNTALVMKRHEI
                     IRLNYTGNNPCPYSYQKLLGAYGNRFRNADVTDDSRIFITKLSGGFLEYDISNNPWLL
                     TDRQTGHSVHSSFKSDLTGKKYFYSKDHFANGDLFIDENTGGSSLINYPLTNPMGDCI
                     FNPFQNQVLISDFSGSPYLYLKAYSEIPGGLNTQHSTITGNATFNGKMTISRHGELLL
                     LTGVNNVEPKIKIFDAASANYNSMGEIDISGHFTGSEWLEVLYADNSDQTYFVVGDAY
                     DPDAERSLPETQSTSYFYVLDPTDYPESPYIEIQHDIPRFLETMQEGILDETTIYKAF
                     VAGTEHYFSEIIIEDQYLEMIVNHKQVMNPITALKLISYYGESGIVNKLYITTWDGSS
                     AAIWTYDPQHAENEDGLDEIAQIDTYISKMDYNEKTLLLYLYGVENDELKIVQMDVLE
                     QFDGEVKDLNIHSLPSDKLTAVAVKTNELLFDNENHVVDIPNGEFSSLCRLGFAFDRL
                     HLKDKISWFSFPRLERDFDNTVPAIPVLERIRPFPSQLYMENLPLQEVDPEYKYKIYS
                     NLQWDGDLNDIRSTFGYKHSTDNQFLSYQPLMGSILDPNTSITVYPGHENWVGYSHIL
                     PQDPLDALAPVLDKLTLIKHHDWACVKVTCPMMGPGQPEPACWLCLNTTPLKYADMLI
                     LECSEIATFQWGEEFTAEKRELQDAEHFVYSETADYTPIFIELDTMEHPVEMGAFIGE
                     VCIGAAVVKSGDSAIMIRGYMPLDTSGVITFQQYYGSEKALPAIVDEYYVKNQETGIS
                     EKRTIDSRERKKFYQVSFKKESSALFNRNPLKLSYYPNPGPDSGILEYFFPEESEVIF
                     EIYDVHGRKLNTHIVSKQVAGNYTTSWLELTHDDTAQGLFLIKMTACGNSVTTKIIMI
                     K"
     gene            complement(23988..25280)
                     /locus_tag="PKZ74_01085"
     CDS             complement(23988..25280)
                     /locus_tag="PKZ74_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM91371.1"
                     /translation="MLPKIEFLPSSEIKAFQQEKLSQQLHLLNGKSPFYKELFRKNHI
                     DISRIKTLEDLANIPVTTKDDLQKHNDDFICVPRNRIIDYITTSGTLGDPVTFAMTDS
                     DLDRLAYNEAISFAVADGSTDDTYQLMTTIDRRFMAGLAYFLGVRRLGAGIIRVGNGM
                     PELQWDSIRRFHPNVFITVPSFILKLIEYAEEKGIDYRSSSIKKAVCIGEPLRNPDFS
                     LTTLSRRISEKWDIQLYSTYASTEMGTAFTECRYGCGGHHHPELIIVEFLDENDEPVK
                     PDEPGEVTITTLGVEGMPLLRFKTGDVCYHYNEPCRCGRTTMRLGPVLGRKQQMIKYK
                     GTTLYPPALYDILDNIDNIVNYYVEVYTNAIGTDEIVIHIGSRLESEAFEKQIKDHFR
                     ARLRVAPQIIFEPAGYIEKTVNPEGGRKTVRFIDRRNK"
BASE COUNT         6573 a         5946 c         6102 g         6782 t
ORIGIN      
        1 cctcaggagg taatagataa ataccctgaa tggaacaaga aatttgtaaa atgataaaca
       61 tattcctgat gctcatccat ttaccgcccc tgttgccacc gaggggaata tagagataat
      121 ctctgaacca ggtcgttagc gatatatgcc accgtcgcca gaattcggcg atgtcgcggg
      181 caaagaaggg aaatgcaaag tttcttagta agttgatacc aaacaatctg gccgttccga
      241 gagcaatgtc ggaataaccc gaaaagtcac cataaacctg gaatgcaaag aacatcgtac
      301 caagcaccag ggtgctgccg gaataaccag atgaatgact gaaaatatca ttaacataaa
      361 tggcgcaatt gtcggcgatg accatctttt taaagaatcc ccagagcatt tgcctgaggc
      421 catccatcgc ctggtggtaa tcgaaatctc ttttcctgat gatctggggg agaaggtgag
      481 tagcccgctc gatggggccg gccactaaca gggggaaaaa actgacaaaa acggcatagt
      541 tgatgaagtc cctttccggt ttgatccggt cgttgtaaag atccaggaca tacgacaggc
      601 catgaaatgt gtagaacgag atgcctaccg gcaggataac ctgaagggtg aaatagtcgg
      661 catggaatcc aaccagtgat agcgcaccgg caaatgaagc agcaaaaaag ttgtagtact
      721 tgaatacgcc gagaaatcca aggttgatcg tgatggagag ccacagccag atttttttcc
      781 gcttcctggt ctctgcttcg tgaatcttga taccggtaaa ataatcgagg aaagtggaaa
      841 agatgagcag gaaaaggaag cgataatccc agctcccata aaagaaatag ctggcgatga
      901 gcaacaggac attctggccc cgaaggcttt ttcgcgcgac aaaccagtac aacaggaaaa
      961 cgaccggcag aaagacagca aatgatattg aattgaacag catagcgctg cagttaagct
     1021 aaccttttat aatatccctt tcaggtaatt ttccgcaaaa tactcaattc cttttgaatt
     1081 taaatgatga tggtcgtaat aatattcagg gataagaacc gattcggaaa aatcgtaata
     1141 gcgggtgccg tattttttgt acatctcttc taaaaagtcc atcgtttcat aatgcccctc
     1201 ccatttccca aagagcgccg gcggaataaa aaagatgacc tcggtgttgt gttcttttgc
     1261 cagttttatc gtttcttcta ctaccaagca gtttcgctgg aattcatcca tcctttcccc
     1321 atcaacgagc agtagtttta atccctcctt gattttcgtt gaatcgatct ctttcagcgc
     1381 ggattttttt tcgtcaagtg aacggggttg caaacggtac catttgcgcg caaacttgct
     1441 ttttacataa taaaacagcc gttcatattt attgtctgat ttatagccga ggtatttaat
     1501 gaagaaatcg agacggaaag gttcaaattc aaaggtattt gaagccctgt tgataaatcc
     1561 cttattcagc agtgtaggtg acatgacata gaagatcctg tctgcagagt ttctcttgta
     1621 ataaaaatat tccaggaaaa aatgctggtc tatagcaccg cattttcctt cgccgatacc
     1681 gatatttatt atctcctggt gcaatatctt ttccattcgc aggtggtttt tatgccggga
     1741 gaagttccgg gcgtgcgaaa tccccatcat caaaagatta taatcatgat tcgatttcat
     1801 gaccagaagg tttccttcca cttcccagtt ccggaatgct cttttgtaaa caagacggga
     1861 actggccagt gccagcgcga tgaccaggat gaggaaggcc aggataaaga ttgccgtatt
     1921 gatcaggtat ttcttcatct tttaaaaagc gaaataaata aattcggttt tattaaaatt
     1981 accgaagata aaaatgacca gcgccagcgc cagcatgaag ctccatttca gcgatctgtg
     2041 gagccgtacc tcattgaact gcaatccata ctgtttttca cggaatatcc actcaaatac
     2101 aagcaaaatc aggataaaca cccagatata atgagttctg attttcgggg cggtgaacag
     2161 ggaagcgctg aagatatcct ggatgaagat taatgcccca ctgacactgt cggcccggaa
     2221 aaacacccag gcgatcaaag taagcgaaaa agtgacgacc atcccggcaa gatccctcac
     2281 tgagggcaac catttccctt tagccgcgat ttccaggttc tgccggtttt tattcagtaa
     2341 aagcagcggc ataaaataga gtgcattcag gaaaccccag acgatgaagg tccagttggc
     2401 gccatgccag aatccgctga ccaggaagac gataaacgta tttctgacgc tcatccattt
     2461 gccgcctcgg cttcctccca gcgggatata gaggtaatcg cggaaccatg tggtcagcga
     2521 gatatgccat ctgcgccaga attcggcaat atcacgggca aagtacggga aggcaaaatt
     2581 gcggagcagt gtaatgccga acaggcgggc cgttccgagt gcgatatcgg aatatccgga
     2641 gaagtcgcaa tagatctgga aagcaaacaa aacgacgccg atcagcagtt cgctgccgga
     2701 gaatgcaccg gggttctcga agatcttgtt gacaatcggt gcgcagttat cggccaccac
     2761 cactttctta aacagccccc agatgatctg ctgcataccg tcgaccgcct ggctgtaatt
     2821 aaacaccctt ttttttatga tctggggaag gagatgggtg gcgcgttcaa tcgggccggc
     2881 gaccaggagc gggaaaaaac tcacaaagac ggcataattg acataattcc tttcgggctt
     2941 tatccggtca ttataaagat caagtacata cgacaaacca tggaaagtat aaaaggagat
     3001 acccacgggc agaatgacgt tcagtgtcac gaagccggcc tggatcccga acatgccaag
     3061 caactcggca aaagaagagg cgaagaaatt aaaatatttg aatacgccca ggaatccaag
     3121 gttgatcgag atacttagcc agagccagaa ggtcttcatc cgtccgggtt tggcttcatg
     3181 gatctttatc ccggtgaaat aatccaggaa ggtggagaac atcagcagga acaaaaaccg
     3241 gtaatcccag cttccgtaaa agacataact ggcaataagc agcaatatat tctgccatcg
     3301 aaggcttttc cctgtcgcaa accagtacaa caggaaaacg accggcagaa atatcgcaaa
     3361 ttctatcgaa ttaaacagca tataaattat tctggcctgc cagtattatt aaattcagga
     3421 tattgataat tcccaatacc ttttagctta tccctgcata ccctggtgaa ctctttcctt
     3481 tcatcaccct gaatggtata ggtaacagag agatttgaat tttccatctg cgggctgata
     3541 taaagcaact cttcgaccag ttggaatgcc cctggcatct ggcgcatttt tgttatcagg
     3601 gaatgagcgt tgccggaatc ttcatcaaaa cttaacccga gttcatggaa aaagatgcgt
     3661 tccaggaact cgttgtcgga cccgaacctg gattcccaga acaggccgta tctttcaaac
     3721 aagtcccgcc ggtacacgga tgtagccagc gcaacattgt atttatcaaa ataatcgtac
     3781 ttgccgtcaa cttgctttcg ccggctttct gctaattcaa gcatggcatt ctggtccagc
     3841 aggtccagtt cctccggtgt gcagcgactt ctcaggaaca ggctgaaggt cacaaaggca
     3901 ttaccggaaa ccagcgggat caattgtttc tcgatccggt ctttcagtga tatatcgtcg
     3961 gcatcctgaa tggcgatata ttttcctttt gactgcctga gcgcatcatt tcttgcaaaa
     4021 taacaacccc tgttttgttc gtttcggatg aaaattaccc ggtcatcctc cttcgctatt
     4081 cttttgacga tacctgccgt attatcagtg ctgccatcat caacaacaag gatctcaagg
     4141 ttctggtagg tttgaagcag cactgaccgg atgctctttt cgatggtcct tccggcatta
     4201 tatgccggca tgattacact gacaaggtcg tcctggtatt tcttcagatc atattcaggc
     4261 aggttccatt tcatccgttt ggaaaattcc acctggtcgc gaccaccccg gatatcgctg
     4321 gttcttacat cggcgatgac caggttaggc tgacagatat agcatttctt ccggtattta
     4381 tggtaaatag ccctgaccgg gcctgaatcg aaagagcagt tcattttatc aatttcagct
     4441 atgatctccg ggaacaccga ctgatcatag ccaagggcaa atgccccatc ggtatacaaa
     4501 gggtagtaaa acttcttccc gggatcatat tccgtaatgc ttaaatcatc ataaaacaag
     4561 ctgccgggaa aaagccacgc atgttgcgtg gcccccagcc caacgatttt ccagtcatca
     4621 tcgattgccc ggataaactc ttcgaatttc ctgtgaaaat cttctatgaa gaaaagatcg
     4681 tcctggagga taaggatccg attgtaattg ttttgtatag catcctggaa gatgagtttc
     4741 ttggatttta gcactcccca tgctcccgga cttgagatca ttttccggtt atattttttc
     4801 tcgaggggat gggcgtccgg ctctcccaga ggaatggaca gatatttttc atattcgctc
     4861 ttgtgtggtt cttcatttcc gttgatccca tcgacaatcc aggcattgat ccccatctgt
     4921 ttcaactgga agatcgattt caaccgtttg tcggagcgat gtttcaggtt cagcagatat
     4981 attttgtcaa aataggaatt gatccggttt gcttttttta tctcttccgg aatatggatc
     5041 tccatcatgg aaagctcgac cggtgctttc tttagcctgt tatatttatt tgcaggaatg
     5101 ggttcgcgcg gattgaaggg tttaagtccg aaaagatggc ccagttgggg catgcgtcct
     5161 tctttcagga ctacgactac cctttgcaca aaccatttcg gcgtccaggg tctgtatttt
     5221 tcggttaaaa aactcatgga ttaaattttg gataattata ctgatattca cctctcaatc
     5281 tttggcgcca caacccttcg aacgcatctt tctccttctg gctgtggcgg ttggtaatat
     5341 tatcgccggt catgtcggcg ctgatcaaca gcactttgtc gatccgttcg tagataccgg
     5401 ggatggcgtc cctatccatc agaaagctgt ggatcgtgcc gtctttcttt gaaagcagga
     5461 taccggcttt ttggaacagc acccgttcca ggaattcggc gtcggcgccg aaccggtgct
     5521 cccagaacag ccccaactct tcaaacagct cccggcgaaa catcgaggtc atgaagccta
     5581 tgacaggtct gtcgcggtac tcgtacaggc cggaagcaga ctccatccgc cggtcgagta
     5641 ccagttgggc catctcccgc tggttgccga tgtccagctc atcgatcgag cagcggccgc
     5701 gcaggatacg ggtcagcgtg aacaaagccc tgcctgtcag caacgggata agctgggttt
     5761 cgatccgggt ggccagcgaa atgtcgtcgc tgtcctggat ggcgatgtac ctgccctttg
     5821 ccaccctcag cgcggcattg cgggcggggt agcacccctg gttgctgtca agcctcacca
     5881 gcttcacccg gctgtcgttt ttcgccagcc tgtccacgat agctacggtg ctgtcggtac
     5941 tgccgtcgtc agcgacgatc atctccagct cccggtaggt ttgcatcagg atcgaccgga
     6001 tgctcttttc gatcgtcttt tctgcgttgt aagccggcat gatcacactc accagctcct
     6061 tccggaacgg gtagtcgtac agatccatct gccagcgcag tttcctggcc atctgcccct
     6121 ggtcgcgctc cacgccgata tcgctctggc cgacgtcggc cacaaccagg ttgggcttaa
     6181 ccacgaagca ctgcccgggg tatctctgct gaatcgctct cagcgggccc gaatccagcg
     6241 cacagttcat cggttcgatc tccgaaagga tctcatcgaa taccgaacaa tcgaccccaa
     6301 cggcaaaaga gccgtcggtc ttaacgggat aatagaaagg atgaacgcca aaatcttttt
     6361 cacccacttt gtcaggataa ttgacattgt aaggcacgct ccagtcgtgc tgcgttgccc
     6421 cgaggtacaa aagcttccag tttccgggta tggatttcag cgattccccg gttttctttt
     6481 caaaatcttt atgaaaaagg acatcgtcat caaaaacaag aatccttgca aaccccttac
     6541 ttttagcatc attcagcaga tacttccagg taagaagata tccccatgaa ccggggctgg
     6601 caatcatctt cttcttcagc tccttttcca ggggatggca tccctcggcc gccagcggca
     6661 ggtcgtaata agcggcaaag gcttcggaat tctctttgga atacccgttc ccggcctcga
     6721 atatctccgc tttgatgcaa tatttcctga gcttcatgat catctccacc cgccggtcga
     6781 gccgccggcc caggttgatc acatatatcc tgtcaaaaat atgattgatg accgataatt
     6841 cattttcatc aacgtcatca ttaataataa agggcttgta ttccagcgtc gggacaaagt
     6901 tgaacaagcc gcctgcgaca tccggatctg ccaaaccggt gttccaggta cggttacgga
     6961 catctcccct gatcttgcgg gtgaggcgga aatcgtacat cagatcgacc atcctgacct
     7021 ggggcagtct tcgctctttc atcaccacga tgaaacgctg tatcacccag aggatcgtcc
     7081 aggggcggta tgttttccgt aaaaaagcca tctttctgtg cgtcattcaa ggtccatgat
     7141 cttctcactt acttttttgc cgccgtaaat ggaactgtat ttcttgacaa cttcagccgg
     7201 atcaccatcg atgaccagct tccctttgtc gatcaggatg gcgcgtgtgc ataaagcttt
     7261 aatcgattcg ggggaatggg aaacaaacag gatggtcttc ttttgattga agaactcctt
     7321 tatcttatcg tagcttttgg ttttgaaggc cgcatcgccg accgacagca cctcgtcgag
     7381 gatcaggata tcgggatcta tattcacgga gacggagaag gccaggcgtg ttttcattcc
     7441 gctgctgtag tttttcagcg gctgatggat aaactcgcca atatcggcga actcgatgat
     7501 ctcggccgtc ttttcaatca cttttttctt cgggtaaccc aggacggcgg tataaaaata
     7561 gatattctcg tagccggtga attcacggtg aaatcccgaa cccagctcca gcagcgggac
     7621 caggttccct tttaccgtta tcgtcccttt ggttggctgg atcaccccgg ccaccagttt
     7681 catcagggtc gatttgcccg atccgttccg gcccatgatc cccaggatat cgcctttatt
     7741 caccgtaagg ctgaaatcct taatagcata aaaatcggta tgttttctcg gaaggaaagg
     7801 aaacagagcc tggagtgcct ggtctttttt attccggtac agcttgtatc tcttgctgac
     7861 gtttttaata tcgattatga tcggttttcc cattttcgat cccccttata ccatttcagc
     7921 aaaatatggc ctgagccgtt tgaataccag tactcctatt ataaaaaatg cgcctgccaa
     7981 tccccagaaa taagcagtat cggtccagtt cgaccagaag gggatcccgt aaaggaagct
     8041 gtcgcggtaa ccttttacaa tgtaatacag cggatttaac ctgaaaatga ggaccatgtt
     8101 ttcgggtatg tttttcattt cccagaaaat gggcgtaaag aaaaacaata ccctcatgac
     8161 aatggtgatg atgtatttaa tatccgggaa gaacagtgaa actgatgaag taagccaggt
     8221 aaggcccacc aggaggaata cagtggcaaa gagataataa aatacctgga gccagtaaat
     8281 ggttaccggt atcccgctta atatcaggat ggtagagact attaccagga ttatcccatg
     8341 cataatgagt tccgaagaga ttttaacgag cggaatgatg gccgacctga aatggacttt
     8401 attgataagg aatccataat tgctgatccc ccgggtggct gagttcagcg ctttgttgaa
     8461 caggtcatac gcaattaaac ccgccagtag aaagagggca aatggtacag aggtcttttg
     8521 cccggtgcgt atgaaagtga atacaaacca gagaacgaac atcatcgcca gcggttcaag
     8581 tatagcccat gataaaccca ggatattgct gacgtagctt gtcttgaagt cgcgtttggt
     8641 cagctcccag ataatgaatt tattgaggta gatctgtttt agaaaatcgg ccccttcaat
     8701 aaaaaaacgc tttatcaggt aaatcagttt cactgcttaa atacagaccg gcttaatgtt
     8761 gaacggacaa agatagcatt ttatatgttt gcgccaggcc ttcttcaagt gagatgcggg
     8821 gtttgaaccc gagcttgcgc aaacggctgt tatccatgat cttccggggt gtgccatcgg
     8881 gctttgaagt gtcgaaaagg attttgcccg tatagccggt gattttagcg atcaggcggc
     8941 ccagttccag gatcgtaatg tcttctccca cgccaatatt gatgtgggtt aatccaaggt
     9001 cgtcgtaaag cttcggtgcg tcgagcttct ccatcaccag gatgcaggcc cgggccatgt
     9061 cgtcgacatg catgaactcc cgcaacggtt ggccggtgcc ccagagggtg acggaagggg
     9121 cattgctgat catggcatcg tggaacttgc gcagcaaagc ggggatcacg tgcgaattct
     9181 gcaggtcgta attgtcgttc gggccgtaga ggttggtcgg catcaccgag ataaagttgg
     9241 agcggtactg ccggtagtaa ttctcgcaca ttttgatgcc ggcaattttg gcgatggcat
     9301 acggctcatt ggtcggctca aggctcccgg tcagcaggta ctcttccttc agcggttgcg
     9361 gggccagctt cgggtaaatg caggagctgc cgaggaaaag gaactttttc accccgtttt
     9421 tgtaagatga atggatgaga ttgttctgga tcatcaggtt ttcgtagatg aactgggcgc
     9481 ggaaggtgtc gttggccaca atgccgccca ctttggccgc cgcgtcgaac acatattcgg
     9541 gctcttccgc ctcgaagaag gcctccacat cgtcctgccg cgttaagtca agttcatcga
     9601 aggtccggaa gatgaagttc ctgtaacctt tggcttcgag attgcgatgc agcgccgagc
     9661 ccaccatccc gttatgcccg gcgatgtata ttttcgaatc tttattcata atagttcatg
     9721 atttcatatc ccccttctcg caggaattgc tctttccgca tcagttccag gtcggcctcc
     9781 atcatttcac cgatcaggtc gccgatcgaa agttccggtt cccagcccag ttccgctttg
     9841 gctttcgagg cgtcgccctg cagaatatcg acctccgttg gcctaaaata gcgcggatcg
     9901 atttcgacga cggattttcg attttcgatt ttcgatggtc gattttcgat ttttttccat
     9961 cgaccatcga ccatcgacaa tggaccatca accatcttgt tgaggtagcc tttctcctca
    10021 acgccttccc ccctgaactc gatctctgcg cctatgtaag caaaggcttt cttaacgaac
    10081 tccctgaccg acgtagtctt gccggtggcg atcacgtagt cgccgggcgt gtcctgctgc
    10141 agcatcaggt gcatggcttt aacgaagtct ttggcatggc cccagtcgcg ccgggcgtcg
    10201 agattgccca ggtagagctt atcctgcaag cccatggcaa tgcgggccac tgcgcgggtg
    10261 attttgcggg tcacgaaggt ctcgccgcgg ataggcgatt cgtggttgaa caggatgccg
    10321 ttgcaggcga agatgttgta agcttcgcgg tagtttacgg tgatccagta ggcgtagagt
    10381 ttcgcggcgc cgtaaggcga gcgtgggtag aacggcgtct tttccgtttg cggtatctcc
    10441 tgcgccaggc catagagttc gctggtgctg gcctggtaaa aacgggtctt gttggccagt
    10501 cccagcaggc ggatggcttc gagcaggcgg agggcgccga gcgcgtcgga gttggcggta
    10561 tattcgggtg tttcaaagct taccctgaca tgcgactggg cgccgaggtt gtaaatctcg
    10621 tcaggttgca catcctggat gatgcggatc aggttggtcg agtcggtcag gtcgccgtag
    10681 tgcaggatga atttctggtt ctccacatgc ggatcttcgt aaagatggtc gatcctgccg
    10741 gtgttgatca gcgagctgcg ccgcttgatg ccgtggacga tgtagccttt tttcagcagg
    10801 tactcggcga ggtaggcgcc gtcctgtcct gtgatcccgg tgatgagtgc ggtcttgggc
    10861 atagctggct gagtttgcgt tttgagtttc cccggaatta atcccgggga aactcaaaaa
    10921 ctgtgctaag ttaaacatat tattctggac taaaacctgt atttttgatt tatggaacga
    10981 tctataccta ttgtagtcgt tggttataac cgcccgaaag cgttgaaacg gctgctaaat
    11041 tccctgctga aagcggacta tccgtgtgag gtggagctga ttatcagcat tgatggcggt
    11101 ggcgatgaag aaaataaaaa aattgccagt tcattctcct ggccatttgg caaaaaacaa
    11161 ctcatctttc atgaacagaa tatcgggttg cggaagcatg tgctttcttg cggcgacctg
    11221 gtcaaagatt acgacggggt gattgttctg gaggatgatc tgtatgtgtc gcccgggttt
    11281 tatgaatata cgctgaaggc ttttgatttt tataaggatg atgataaact agcgggcatt
    11341 tcgctttata cgcatgctta caacgaaacg gcccagttcc cgttccggcc gctggcggat
    11401 ggttcggatg tattcttcct gcagtacgcg ccgtcgttgg gacagttctg gagcagggaa
    11461 gggtggaggc gcttccggga ttggtacgat acgctggtcc ccggcgagcc gatcccggtg
    11521 aacctgccgc caaacattaa actctggccc gaaacctcct ggaagaaata tttcatcgct
    11581 tatattctcc gccatgatct ttatttcgcc tatccccggt catcgtttac gacccatttc
    11641 agcgacgagg ggacgaatat gcgggtgaag gagaacctgt tccaggtacc gttgtggctg
    11701 gataagaaag tgtttgtatt cagggctttc ggggattcga atgccgtgta tgatgttttt
    11761 tgtgagatga aaggcgagaa gctcaggcaa cgggtgcctg cgctggcagg ttatgatttt
    11821 ggtgtcgacc tttacgggat gaagtcgaag gaagatacgg acggggaata tttcctgtcg
    11881 cagcggcggt gcaaaaatcc cgtgatgcag tttgggcggg agatgcggcc gcatgagatg
    11941 aacatcacgg agggcattga aggggagtat ttttcgttgg ggaagaagga ggattttgaa
    12001 gaaaagccat ttatggtgag gcttttaaag tgccatgaaa agcaggagct ggcctactgg
    12061 tacccgatcc gggagtatca tttttataag aacaggttgc tgacgaccgg aaagacccag
    12121 aacttttttc tcaatcctgt tttcctgttc cggaagttta tggtgatggc gaggtatgcg
    12181 tacaggtact ttcggatttg acgatttgta tcagggtttc caacccttta ttatttgcac
    12241 aatcgcttga tatataatta tttgatctga aagttagatt tgccatacaa gccacctaaa
    12301 atttaaacta aagaaaatca acgttttacg gtttactcgg ataattcaga attcagggtt
    12361 ggaaaccctt ataaccaacc aatccagcat gaaaaaccct gcaaacagaa catagatcag
    12421 cgtcatccct tcccagggcc gcagaatgtg aaacgaacgg cccggatagg cctctttcca
    12481 gaattcatcg ggcatggcct cgcggttcaa cgaaaacagg ttatcgccac ccggcatggg
    12541 atcggctgtc atcaaacctg taaccatcaa caaaatgaac gacgccagca ttaccagccc
    12601 ggtgatcacg gccggcgacc ggtaccacct caaatatttc atctttataa ccccgttgtt
    12661 gacgataaat accgtaaccg ccgccgtacc ggccccgaga aggttcagca cgttgtcgtt
    12721 gaaatcgaaa tagccaaact cggggacgag gaaaaaatac tggaacagct catcgagcac
    12781 acccaggagc gttacccaaa aaaccgttcc cccatagctc ctggccaccg gcaatagcaa
    12841 gaaggccacc aggaagtact ccacgaaatg gatcgcctcg atgttgtaca ccatcaggta
    12901 gttgaacgcg gcgatcatca gcccggccat aacgacgacc agggaggtgt tcagccagat
    12961 cctccggctg cgtccgatat gccaggccat ccaggccagc accgccagca gcagcgcaat
    13021 gaaaaaccag gccaggtaag cgttgtaatg gtcgcgaccc acagcattgc gcactcgtat
    13081 ggccacctgc gtgatctcgt cgtggaaggt gacgatcgaa aaataggtga tgactgccag
    13141 caggatggct atcgctttgt gtctgccgag ccaggtgagg aggtgtttca tttggcaaag
    13201 ttaggagtta aacagcttag ttttccattc accaaagtgt acacagagtt gagagtgtct
    13261 caaaagtctg tttttttacc acaaaggcgc acaaagtatc agattatcaa ataataaact
    13321 ttgtgttcct tcgtgaaatc cttggtgttc ctttgtggtt tattacttat gatacagcct
    13381 cactacaaac caataataac cgacgggacg ctcgtcggac taccaataaa tagcgaatat
    13441 ttgtactttt gagacaccct caaaataaaa aaagctaaca aaaaggaatt atattcagta
    13501 aacaatactt tcgtagaaaa attagaaccg gtgactaaga tctccatcat aaccccttct
    13561 tttaaccagt tgcacttcct gaaggaaact gtcgacagca tccataacca gcagggggat
    13621 tttgctctgg agcatatcat tgtcgacggc ggctcaaccg acggtacagt ggagttcctg
    13681 cgctcgctcg gcgactgtgt ccgctgggtt tcagagcccg accgcgggca gtcggacgcc
    13741 gtcaataaag ggatgcaggt ggccacgggc gacatcatcg gctggctcaa ctccgacgat
    13801 ctctatatgc cgggggcgct gcaaacggtc gttcgtcatt ttcacgccaa tcccgattgc
    13861 caatgggcct atgggcgctg tatcatcatc gacaaagagg gcgtcgagcg gtggaagtat
    13921 atcacccgtt ataaaaacat ccggttaagg aaattcacac tggataagct ttatcgcgag
    13981 aacttcatct cccagccggc ggtctttttc cgcaaagatc tgtggaacca ggcgggaggg
    14041 ctagatctct cactgcacta cgccatcgac tatgatctgt ggcttcggtt tgcccttcgc
    14101 tcaccggcat tggtcatcga cagcgacctg gcggcgttcc ggcggcacgg cgcctcaaaa
    14161 agcgaaacag gattcaggga ccagttctat gaacagtatg ctgttgcaaa acgttatcat
    14221 ccctcaggat tcaaccggct tatccaccgg ttcaacatct tcaagatcat cacctcttac
    14281 cggatcatcg gcctgttttt ccgcttttaa tttttccagt tcattctgca gcagcgccat
    14341 ctcctgggcc agcactttgt ttttttccga aagttttgag atattgatgg aaaactcaat
    14401 gaggatgagg aagatagcca gtaaaagaat aaggaacagt gccgcagggg gataagcgat
    14461 acccatcagg ccggcgaaat attcgagccc ttcgcgccag atggaaaaaa caatgaagac
    14521 gacgctggag aatagccaca ggagcgagta ttcttctttt atcttctttt ttcttacaag
    14581 atacaggata aacaggaaca gcccgacact gaccaggatc gcgatgacct gcgtggtctg
    14641 gatatttttc ccgatagaac ccagcgactc attcataacg gtaaacttta gaccggatgg
    14701 ccgacataaa catagaaagc atcactttaa tcatgtaata tggccctttg gtaatgggga
    14761 tcgacgatac gccgcccttc cgttcgtgca tctgggtaaa ggtttccctg atcgtgaatc
    14821 cgttgcgccc caggaggatg accacttcgg gctcaggata atcgctcggg taataatcgg
    14881 ccaggaactt caatgctttc cggttgtagg ccctgaatcc cgaggtatga tcggtgatcc
    14941 gctgccggat aagaatgaat gaaaaccatt cgaatatccg gatgcccatc cgtcttgatg
    15001 aggaggattt gaacccgtcg tgcttctgcc ggaacctgga acctatggct acatcggcct
    15061 cgccgctgcg caccagctcc agcaacttgt ggatctcatc ggcactgtgc tgcccgtcgc
    15121 cgtcgaactg caaagcaaaa tcgtacccgt tcctgcgggc ataccggaag ccggtctgca
    15181 cacacccgcc gatccccagg ttgaacggca ggtcgatcac ctccgcctgg ccggtcgcac
    15241 gggctatttc gctggtctta tcttccgatg cgtcgttgat caccaggata tcccagcctg
    15301 cattctcgct gtgaagcttc gaaatgacct cccggatatt ctccgattca ttaaatgcag
    15361 gaacgatgac cagtatcttt ttcatcgcat tcttccggtt cggaaaattt tatttgcaat
    15421 attgctgaat gtatgatctg gcggctttat accccttcgc aaccgatatc tcccagtcct
    15481 tgcaggctcc gtctttatca ttaaatccct tttgtttggc gtacccgcgg ttgtaatagg
    15541 cctggccgaa attgggattg agctgaatgc acttgtcgaa attcccgata gcttctttgt
    15601 actgctggag cgaaatttgt gaaacccctt tgccgttgta gaacaggtgg ttcctgggat
    15661 ccatcttgat cgcgcggtca atatccttga tcgcctccac atggtcgccc atctgcgccc
    15721 gggtcaaacc ccggttgaaa taacaaaggg ggaagttagg atcaattttg atggaagtgt
    15781 tataatcctc cagcgcgcct ttcatatcct tgttttttct tttggaattt gccctgttgt
    15841 aataaaccga cgaagcatta ttgccgagct ggatactttt gttatagtct ttaatggcct
    15901 tgtcgaactt attcatcgcg gcataggtga tcccccggtt gtaataggcc gttatatagt
    15961 ttggatcgag cttgatcgct ttattgtagt cttctatagc ttctttgttt ttacccagtt
    16021 ctttcttcag gttggccctg ccgttgtaag ccagcggaaa tcccgggttg cattcgatgg
    16081 aggtattata gctctccatc gctttttcat tgtcctttac ttccatataa taggtggcaa
    16141 gccagtagtg cgccctttcc cgcttgggcg atttcttgat gctgtcggtc cagagggtgt
    16201 atggattctt ccatacgctg ttgcggttaa gggaagcacc gatataagcc agggcaagaa
    16261 tggaaagtac aatgattggg tacttgatat ttttcggggc caggaaataa aaagcgagat
    16321 agctgatcac aaagccaaat ccgaacgtgg ccaggtagag cctgtgttcg aagatgaggt
    16381 cgcggatcgg gataatactc gattcgagcg acagggtcag gtagaaccag aagatgccga
    16441 aggaggcgat ataccatttt ttcatgaaca gccagacgcc gagagccaca gtcagcagca
    16501 atacgccgag gctcaccagc tctttaacgc cccagagcga ttgagagaca ggccagtaat
    16561 aatcgagatg ctggttgacg ggcaggatga gcagttgcca gtatttgacc agcacccgga
    16621 actgggtgac gagataatcc gaccggctga tccggtcgaa ctcccgtggc aggccattga
    16681 tgatcagtcc gaagacgatg atgatgccga tgatggaaga ggcggcgatc aggaactttt
    16741 tatcgatcct catttcttta tcgcggatga agaacaactc cacgaggagg aaggcaatgg
    16801 ggaaggtgat ggcattctgt ttcgacagga aaccgaagaa cccggccagg aaggcaccgg
    16861 cataaaaagc ccagggtttc caggcctttt tgtttgacgg gtcgaggtgg gcccggcgac
    16921 cgcggatgta gagcacgatg gaccagaggt agaacaggcc ggccagcacc gccatacgct
    16981 ggacgatata ggtcaccgac tgggtctgga tcgggtgggc gacgaagata aacccggccg
    17041 acagggcgat cagcactttg tagtcctgta tgatcttgtt gttccggaaa atattgagcg
    17101 acatgatctc cagggccagg aggaaggcca taaagccggc gaggatgtgg aagatcaggt
    17161 ttgaaacatg gaagccctgg acggcgggcc tgccggaggg atctttgtcg gccagtttgt
    17221 aatcgacggc cagtgtgaag aaggagaggg ggcggttgcc gacggctttc cagtgcgaca
    17281 gccgggtcac gttttccatt ttcttgatct tttgtccgtc gatgatatgg aaaccgtcgt
    17341 cgaactggaa ggagttgttg aaagagttgg cgtaagccag gatgccgacg atagcgatca
    17401 ggatgacggc atatttttta atcagcggct gcttttcgat catggcagat gacagggttt
    17461 tagtggcaat ttccgtaaaa cggccaaaaa tacaaaaatt aagcgttgag ggaattattc
    17521 cttcaccact ttcaccgttt ccgtttcttt cccatcgatg gtcaggcgga caaagtagat
    17581 tccggcgggc aaagcagttc ctcccgggcc ggcgccgtcc cagtagattt cccctttctc
    17641 ttcggtccct ccattaaaat tgaccagtgt cttaaccaac attccgctgc tgttgaaaat
    17701 actgatattc aacctcccgg ctgatttcaa ctcgtaactt atataagtcc cgaaaataaa
    17761 aggattcgga ttgacgttta ataatttgga acccggaacc tggaacctgg aaccgggtgc
    17821 cgcaggcacc cccacccaag gcccatactc atacgctccc atatcaaccg cttcgccata
    17881 aacgcgggga ttgccggcga tgtcaaaatc aggtaaggtt attccgggag gcaaatcgag
    17941 ggtgccggcg tcgatgcaga gggagccgaa atcgatggaa tagggatgct ctcccgagcc
    18001 gaaaaacagg ggatttgcgt ccagatttcc gttaccccat accaacaggt cattcgtacc
    18061 atacttatta attccttctt gtccgtcttg caccagggaa aagttaatat taatggtatc
    18121 gcgattcact gtctgaaaat cagttaagta tgcctggcat ccgtaattat cgtaaagaag
    18181 acaattatat aaattgacta ctgaaccatc cataattcct atagcgccac ctcttggatt
    18241 gttgacagtc cggttatttg caaatgttga attaataata tcggattgac tggatataat
    18301 agatagtgca acagaacctc catagcctga ccagaacaaa ctgtcggtgt tatcagcaaa
    18361 aaggcagtta ataattttcc ctttgttcaa atcattggta tatgacaggc cagagagggt
    18421 taaggatgta tgtcttctat aaacagatgt atcagggcga ttccctgaga atcgacatga
    18481 aatgaattcg ctgtatctgg gaggttgatc atagttaata aataggcctg ctgttttata
    18541 accataacaa tcttgaaaaa cacaatcctt cattataatc gtgtcatctg aatctgaata
    18601 aaagccggta taatggaaac cggtcgtttc ttttacagca acactgtcca ggaccagttt
    18661 tcttgagtat cctgatgata ttccaccgtc accgaggtta aagtaaccat tgccattacg
    18721 aatcgtaata tttttaatct ttgaatattc ctggccaaat gcaaaccttg ccccttcgta
    18781 cttatcctca caatcaataa tcgtattttc cataccttgc ccgattaaat tgacgtaatt
    18841 ttttaattgg attggggtat gttcaccatt taaaatatta gaatatacgc cttctgccac
    18901 atgaattgtt ttaatgttga tactgtctgt agcaatcttt aataacgcgt aggaaatggt
    18961 tttaagcggg tcgttgattg ttaggccgga attactgtta tttccttccg gtgagacgaa
    19021 caggtctgca tttacctggt caatcttgcc gtgcaaaaca gatagaggag gtcctggtat
    19081 gtgcacagcc ctattactga attttccgat gtaatactgg tcaggattca atacggtagc
    19141 cgtatccagc catatttttg acggggtatc gttataataa atcgcaatat ctgatcctga
    19201 ggaactataa ttcagataga tattatttaa attaatgcta tccaggttta caagcgaaaa
    19261 tccaatgcac aatcctcctc ccgctctttg cgcccagtta ttggaaatag tattccctga
    19321 taattctaat gaagattctg tggagtatat acccccacct acctgcccat aattatttgt
    19381 aatccggcat tgataaacat tcattttgga attttcaagc agaattcctc ctccgctacc
    19441 ataggcccag gggaaaaggg tgctgtgatt ggtgccgaac ccgttctgta tggtcagacc
    19501 aatgatagat gtccataaat tgccggatag cgactggatg cagctccctg tatggttccc
    19561 atcaataacg gtatgggtaa tcaacgagtc ttcatgtgcg taaatccatg tacttgcaag
    19621 gacaatgcct ttatcggtca gatccagatt ttcataataa gtgccgggat aaacaatgat
    19681 agtatctccg tttacagatg catcaattgc ttcctggatt acggtgaaat cgccggttcc
    19741 gtcctgtttg acggtgatga cctggctttt tgccgggatt aacatggcca gaaaaagaaa
    19801 gacggatgcc gtccttgtta atgaggatcg gcatccggaa atcatattcg attgaaatgt
    19861 aatcatttga tcatgatgat ttttgtggtg acagaatttc cgcaggctgt cattttgata
    19921 agaaataaac cttgtgccgt gtcatcgtgt gttaactcaa gccaggatgt cgtatagttt
    19981 ccggctacct gctttgaaac gatatgggta ttcagttttc ttccatgcac gtcatatatc
    20041 tcaaatatca cttcagattc ttccggaaag aaatactcca gaatgccgga gtccgggccc
    20101 ggattggggt aataacttaa ttttagcgga ttcctgttaa ataaggcact gctttctttt
    20161 ttgaatgaaa cctggtaaaa cttctttctt tcacgtgaat caatagtcct tttttcacta
    20221 atgccggttt cctggttctt gacgtagtat tcatccacga tggcaggcag tgctttttcc
    20281 gatccataat actgttgaaa ggttataacg ccggaggtgt ccaagggcat ataccccctt
    20341 atcattatgg ctgaatctcc tgatttcaca acggctgcgc caatacaaac ctcgccaata
    20401 aaagcgccca tttcaacggg gtgttccata gtgtcaagct cgatgaaaat aggggtgtaa
    20461 tccgccgtct cagaatatac aaaatgttcg gcgtcctgca attcccgctt ttctgcagta
    20521 aactcttctc cccactggaa agttgcaatt tctgaacatt cgaggatcag catatcagcg
    20581 tatttcaacg gagttgtatt caggcaaagc caacaggccg gttcaggctg gcctggcccc
    20641 atcattgggc atgtaacttt aacacaggcc caatcatgat gtttgatcag tgtcagcttg
    20701 tcaagcacgg gggcaagggc atccaaagga tcctgcggca agatgtgaga atacccgacc
    20761 cagttttcat ggccgggata aactgtaatg cttgtgttag gatctaatat agaacccatc
    20821 aagggttggt acgataaaaa ttggttgtct gtggaatgtt tatatccaaa agtgctacga
    20881 atgtcgttta aatccccatc ccattgcaag tttgagtata ttttgtactt atattctggg
    20941 tcgacttcct gtaatggcag attttccatg tagagttgag aagggaaagg gcgaattctc
    21001 tcaagtaccg gaatagcggg cacagtatta tcaaaatccc gttcgaggcg agggaaggag
    21061 aaccatgaga ttttatcctt caggtgcaaa cggtcgaagg caaaacctaa cctgcataac
    21121 gaactgaact ctccattggg gatatcaaca acatggtttt cattatcaaa caacagttca
    21181 tttgttttta ccgcaacggc tgtcaattta tcggaaggta aggagtggat atttaaatct
    21241 ttaacttcac catcaaattg ttccaaaaca tccatctgga caattttaag ttcatcattc
    21301 tcaacaccat ataaatataa cagaagtgtt ttttcattat aatccatttt tgagatatac
    21361 gtatcgattt gtgcaatttc atccaggcca tcctcgttct ccgcatgctg cggatcatag
    21421 gtccatatcg ctgcagaact tccatcccag gttgtgatat atagtttatt tacaattcct
    21481 gattcaccat agtaacttat taattttaga gcagtaatcg gattcatcac ttgtttatga
    21541 ttgacaatca tttccagata ttgatcttca attattattt ccgaaaaata atgttcagtc
    21601 ccggctacaa aagctttata tattgtagtt tcatctaata ttccctcttg cattgtttca
    21661 agaaacctgg gaatatcatg ttgaatttct atgtaaggac tttcaggata atcggttgga
    21721 tcaagcacat agaaatagct tgtgctttgg gtctccggta atgatctctc agcgtccggg
    21781 tcataggcat cgcccactac aaaataagtt tgatctgaat tgtctgcgta caatacttca
    21841 agccattcag atcctgtaaa atgccctgaa atgtcaattt ctcccattga gttataattc
    21901 gcggaggccg catcgaaaat ttttattttc ggctcaacgt tgttcacccc ggttaatagt
    21961 aatagttccc catgccggct gatcgtcatt ttaccgttaa aagttgcgtt acccgttatc
    22021 gttgaatgct gtgtattcaa tcctcccggg atttccgaat acgcttttaa ataaagatag
    22081 ggcgaacccg aaaaatctga gatcagtacc tggttctgaa aagggttgaa gatacaatct
    22141 cccatgggat tagtcagcgg ataattgatc aacgaagacc caccggtatt ttcatcaata
    22201 aacaaatcgc cgtttgcaaa atgatcctta gaatagaaat attttttgcc ggtaaggtca
    22261 cttttaaatg atgaatgcac tgaatggccg gtttggcggt ctgtcagaag ccagggatta
    22321 tttgaaatat catactcaag aaacccgccg gataatttag tgatgaaaat cctggaatca
    22381 tccgtaacat ctgcattcct gaaacggttg ccataagctc ccaaaagctt ttgatatgaa
    22441 taagggcatg gattgttacc ggtataattc aatcggatga tctcgtgcct tttcatcaca
    22501 agtgccgtat tattggaaac ggattcaatc gccaatggca cattctgtct gactcttgga
    22561 gggtcatagg tgtttaattc gcattttaaa acaggaggcg aagtattgtt ctgaaactga
    22621 aaaacataaa catcattaaa ttccgttacg gctggctctg cataggctcc aaggaaaaga
    22681 tgattatggt ggtcattaaa tccggcacag gtataccctg cggcagggga taacatagta
    22741 agcggatcat gagatgatat atccccttca tatattttaa cagatgttgt tgttgaagga
    22801 ggatttgcac cgggcagaca ataaacctgg tgtacgtcat ttgtgtgaac ataccggatc
    22861 aggccgggtc tgaaatttac aggtacctgc ttgccgggta ctggctcgaa atcgtcccga
    22921 tcgagcaaag cccaataacc agtatcatct ccggtaaaaa ctccaacata aatgatattg
    22981 ttgctgacaa tatcattaat tgcgaaatcc gtaatgatat aatcaaggca ttgaataatc
    23041 tgaaccggat aattagtaga agataaattt accgtgcaaa gccatgattt ggaatttccc
    23101 gtattattgg ccatacctct gatcatccat actatacggg aatagtcatt atcgtacact
    23161 aatttggcgc tgcttaattt atatcctatt aatccacttg ggatttggca ttgatgaggt
    23221 gtgtcatcgg ttacatcaat gtaccacagc cgaagatcag gtccgacggc aaatatttgg
    23281 ccggggcaat tttcatccga tatatatatc atcgggacac gggcaggaag ctctaaaaaa
    23341 tcagtaaacg gtctgtcata aaatccggaa acctcattcg taaactcaat cttcacagga
    23401 acttcttccc aattggtcag attcagatta taaatcaaaa caccttgcat tccgtacgca
    23461 tacagcttat gaatatgtat cccgtttata taatcatcca cctccagggt atattcagtc
    23521 atgaccccga gattattctt cggtaggtaa ataatttcag gagtacctaa aaaaggatcg
    23581 ttttgagaaa atgatggata tgctggcaat agtaaacata agcaccacaa tattttcact
    23641 aagatagttt tcatggtttt ataaaattaa atgaattatt cgattaatta aaactggcat
    23701 caattaatca aataattagg gcggaaatac tttctcagga agaaagatta ctctctctct
    23761 ctctctctct ctctcttacg taaatgccac ttcaggcaaa accgtatcat cgggattaat
    23821 tgaaattcac taaagatcaa tggttggggg gcgggacaaa tataataaat atcgatagga
    23881 aagtcaagtg aggggtcaag tgagactcca tctgctataa gtgagatgga gtctcaaagc
    23941 gccgagactc catctcattc tgcccagatg gcatctcaat ccaaatccta cttattcctt
    24001 cggtcgataa accgaaccgt cttgcggccg ccttcgggat tgacagtctt ttcaatgtat
    24061 ccggcgggtt cgaaaatgat ctggggcgcc acgcgaagcc tggcccggaa atggtctttg
    24121 atctgctttt cgaaggcttc cgattcgagg cggctgccga tatggatcac gatctcgtcg
    24181 gtgccgatgg cattggtgta aacctccacg tagtaattga cgatgttgtc gatgttgtcg
    24241 aggatatcgt acaaagccgg cgggtagagc gtggtgcctt tgtacttgat catctgctgt
    24301 ttgcgcccca gtaccggacc caggcgcatg gtggtgcggc cgcagcggca gggctcgttg
    24361 tagtggtagc acacgtcgcc ggtcttgaaa cgaagcagcg gcattccctc cactcccagg
    24421 gtggtgatcg ttacctcgcc cggctcgtca ggcttcaccg gctcgtcgtt ctcatccagg
    24481 aactcgacga tgatgagctc gggatggtga tgcccgccgc agccatagcg gcactcggtg
    24541 aatgccgtcc ccatctcggt cgaagcgtag gtggaataga gctgaatatc ccatttctcg
    24601 ctgatccggc ggctgagcgt ggtgagcgag aagtcgggat tacgcaacgg ttcgccgatg
    24661 caaaccgcct ttttaatgct cgatgaacgg taatcgatcc ctttttcctc ggcgtattcg
    24721 atcagtttga ggatgaacga cgggacggtg atgaatacgt tgggatggaa ccgtcggata
    24781 ctgtcccatt gcagctccgg catcccgttg ccgacgcgga tgatgcccgc accgagccta
    24841 cgtacgccca gaaaataggc cagcccggcc ataaagcgcc ggtcgatggt ggtcatgagc
    24901 tggtaggtgt cgtcggtact gccgtcggcg acagcgaacg agatggcttc gttgtacgcc
    24961 agcctgtcga ggtcgctgtc ggtcatggca aaggtcaccg ggtcgcccag ggtgccgctg
    25021 gtggtgatgt agtcgatgat ccggtttcgc ggcacgcaga tgaagtcgtc gttatgcttc
    25081 tgcaggtcgt ctttcgtcgt aaccgggatg ttggccaggt cttccagtgt cttgatcctg
    25141 gagatatcga tatggttttt ccggaacagc tctttataaa acggcgattt cccgttcagg
    25201 agatgaagtt gctgggatag tttttcctgc tggaaggctt ttatttcgga agaaggaaga
    25261 aactctattt tcggaagcat ggcgggaact tgggatattt tgattttatg attttatgaa
    25321 gttgtgaact tatgaactta tgaacttatg aatttgtgaa tttgtgaatt tgtgaatttg
    25381 tgaatttgtg aatttgtgaa ttt
//
LOCUS       DAOPNH010000008        25000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_1797_length_25000_cov_6.526398, whole genome shotgun sequence.
ACCESSION   DAOPNH010000008 DAOPNH010000000
VERSION     DAOPNH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25000
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_25000_cov_6.526398"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PKZ74_01090"
     CDS             complement(<1..1404)
                     /locus_tag="PKZ74_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM91372.1"
                     /translation="MNLLRKLVLPAIAALLIHSCPALSQILINEYSASNLSFIQDNYD
                     EYEDWIELYNAGPSGVNIGGYHLSDDTASPALWTFPAGVFLPSNGYLKIWASGRNEVS
                     GNHYHASFRLTQTKSSPDFVVLSDPDGVIIDQVQLAITQKDHSRGRAVSGGDQWAIFS
                     TPTPGSSNGNSTAYSAYAKKPVVSDTGGFYSNPVTITLMNPEPNSKIRYTLDGTEPYS
                     YATEYTAPISIFSTKILKARIFSNDPSILPGLIEFNTYFINANHSLPVLSIAGNELLE
                     LLNGNSALRPRGSIEFYNKLKQRTTTAYGEFNEHGQDSWVHPQRSIDYITRDEAGYNY
                     ALQQKFFSLSDRDEFQRVIIRAAGDDNYPGIDTSAHLRDDFVQTLSQKSGQHLDWRKS
                     ERVVIYANGQYWGIYALREKVHDHDYTEYYYDQGKYDIQFIMLWGGTWAEYGGQQALD
                     DWYVLHDFIMEHDMSNPF"
     gene            1755..2351
                     /locus_tag="PKZ74_01095"
     CDS             1755..2351
                     /locus_tag="PKZ74_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91373.1"
                     /translation="MVLNQFGRIAQNEWYKLPDRFYNLQLGAFQVMPNHIHAIIVLHD
                     HTENDTAAGATLAVAPAAVGPIDNDPVAPVDDVYPVAPIDVYPVAPVDDVYPVAPVDD
                     VYPVATDNNFATAGAGASPAPAVSPSNPTVGNIIGAYKSLVANECLKIFKSKNEKMGK
                     LWQRNYYERIIRNDRAFNAISNYILNNPAKWNAKKSIS"
     gene            2708..3745
                     /locus_tag="PKZ74_01100"
     CDS             2708..3745
                     /locus_tag="PKZ74_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPM91374.1"
                     /translation="MKIKPASVLFHVIGCTIFLLLPIIFWPGEGGISDFFNDSRAIRG
                     YSVNILVLIFFYLNFYYFIPRFYFKKRYAVFILILFAFYLVIIFLPELIVPFNEPPRR
                     MPPGPEPGGKHLIGSGKWFFRMGHHLVFFLAVVFFSLLIKIHNRLKLTEQEKLQAELS
                     YFKAQINPHFLFNTLNSIYSLAIQKADNTPGAVVKLSGMMRYVLSEASKEMVPLDREI
                     GYITDYIELQRIRFGETVKIDYHPCETAPGKVIAPLLLIPFIENAFKYGVNPEADSNI
                     TVRVILYENDLHLHVFNYKVQDRKEDEVPGGVGVNNARKRLGLIYPGKHKLDINENEF
                     EYKVDLYIDLG"
     gene            3742..4425
                     /locus_tag="PKZ74_01105"
     CDS             3742..4425
                     /locus_tag="PKZ74_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91375.1"
                     /translation="MITAIALDDEPPALRIVENFCGKVDFIRLEKSFTHPHEALKHLE
                     KFPADLLFLDIRMPSMSGVEFAKKVPGELMVIFTTAYSEYAVEGFNLNAVDYLLKPFT
                     FERFMQAVNKAGDYYRFLHQSEQALQQYLFVRADYSLVRIILDEILYIEGLDDYLRIF
                     LTNRKPVVARMTMKTMLEKLPADRFARVHRSYIVPVSRIDKVRNKIIFIGDKQIPIGN
                     SYEKGIFRE"
     gene            4513..5571
                     /locus_tag="PKZ74_01110"
     CDS             4513..5571
                     /locus_tag="PKZ74_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91376.1"
                     /translation="MRISRRHILIILFVLIVITIFLPIPVRYSFIATAKIYPFREWKL
                     MRGTEEGFWSQTFNYETDALGDFKNYRFERGDIAELEINEKLVFDMPVSQHDTIAHIR
                     SFFIENEITRLENLRDVETANKDVVSTGEKRPLVEQAQRQYNYALQQLDLEKKNFARQ
                     EKLFRDSVITPAEFDLYENTLKLAEINAQVAFEALTSLQTGEKDPVVNLSEQKITSYE
                     KEVERLKTQKAQYTIVTPISGILSYNTEIGGIIKVSDSEKLLLKIPVAYEHSSYLNNL
                     YKVTFSTPDNKITVNASFKGFDESVNQIQNKQFVIARAVTTGPTTGIYPGMVVQCRIY
                     CDKVYLLEYLKRNFSVSF"
     gene            5574..6335
                     /locus_tag="PKZ74_01115"
     CDS             5574..6335
                     /locus_tag="PKZ74_01115"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPM91377.1"
                     /translation="MKYEFKYVVPVSQLDALREAIKPYVEVDPYAEATHLNEYTVRSI
                     YYDTARFDYFFEKVDGYKMRKKIRIRGYNDQKGGDTVFMEIKRKFKEPIEKDREKLTF
                     EVMKRLMAGEGAAAYGDVPDDHGVNGAGKFLYHVYRNNLKPVVLVIYEREPYFDRFKT
                     DVRITIDKNLRSIAYPTLDDLYSENKVIPAIPGRFIFEVKFKDQFPVWLRPITGHLGL
                     VRRSASKYVFCIDSHRLTEPSKKSDMFRFMHWNRL"
     gene            6346..7032
                     /locus_tag="PKZ74_01120"
     CDS             6346..7032
                     /locus_tag="PKZ74_01120"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPM91378.1"
                     /translation="MLEEFTNIELFPLNVYTVLTNMGVSLACGIVIALVYRFIYKGPS
                     YSATYVNSLVLLSLITTIVIMVIGNNLARAFGLVGAMSIIRFRTAVRDVQDIVYIFFA
                     LTVGMASGVGLYAIAISGTILICLVSVVMVTFNFGAPGRAEYVLQIIYKPSKDTEDRI
                     AGILQKYCRGIRLSNLKSQGENDGIEAFYQFSLRRKYKSTELVEEFSHLSEKVKFNLY
                     FDQDEANIAF"
     gene            7162..7521
                     /locus_tag="PKZ74_01125"
     CDS             7162..7521
                     /locus_tag="PKZ74_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91379.1"
                     /translation="MKAKFFFFFILFLGFSAITFAQATTADPVEKKDAPAVVTSPDHA
                     KTGECTGHTTTAKADCKWVDANGDGKCDSCGKTEKECKEACAPKAAATTPKSGCASTC
                     PHAKECGKSTGTLPKKD"
     gene            complement(7680..15521)
                     /locus_tag="PKZ74_01130"
     CDS             complement(7680..15521)
                     /locus_tag="PKZ74_01130"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPM91380.1"
                     /translation="MKIRLSYTLILLVISLFTNAQVAEYYFTQQNGTYTEINGGTELW
                     ATSFDNEVSGAVTIPSFTYDGTAYTAIYISVNGFITFGSAPAGTVYNPIGNTATYSGA
                     ISAFGRDLNQAASGTPAVRYEQVGSEFVIQWKDVRRINITGEIISFQVRLNTSNNTIK
                     VVYGGTITPGPNTTYPQVGLRGPDNTLATNVHNRTIAAGGGNWINSTRGTGNSNTMYF
                     NSATPATVPTAGLTYTWKPLYNPTAFTAEAVSLTQIDLDWVKNWSGHNVMLAYNTANS
                     FGTPVTGTVYTPGGTVEGTGTVLFYGNGTSFSHTSLSPNTVYYYKIWSYDAVPDYSPG
                     VTASTRTGYALPYLQTFPSTTSPAEWSNNMSRAANHGTSLSIGQYERLTSSNTSCYAI
                     SPLVGSITSDTYLSFHYRFVNYLGFPLNATELGPDDKVDIQVSTDDGATFTTIYTIDE
                     NNHTATTDFTSTAVSLAAYSGDFIKIRFLCTRGSGDYYFDMDNVLFEDGLNMSYSTST
                     TEQPNTTNVGIGTTDNDIIRLQVVTQKSANPLYMNSISVTVNGDIYVNTVKIYYTTTP
                     IFSTSTLFGTQNNPSGGFTMNGSQVLAPGVNYFWLAYDIKSTATAGQTVDGACTGFVT
                     SETGSGKTPTITAPTGSRTIGATFTGTKAIPGDYATIAAAVTALNSGVIGPGGVIFNV
                     AGGHSESVTAPVWLYTTGTASNPITFRKYGAGAKPLITRTDVGSVSTSTIGNNGDAVV
                     MLQGTDYVTFDSIDVTASNSGIEYGYYLRKESVTNACKNVTIRNSTITLTKGASRYVA
                     GICVGNNTSTTNDVGVTSPGGAHENITLTGNTISNVFAGIYMKGSDDYNDHDFTVGVS
                     GFGNTITNFGGNAAFDTWGIYLTKNTNSLIQYNTINNMTGGGTAFSATASGIHHASTD
                     GVSFTAEYNNINLTSASSILYGIYNAVYGPLQLNYNNIALANTASSTAVYSFIHNFQS
                     SIVSSSNITINNNTFAASSIQTTGSTHLIYNHNQRLNPSVTSVQNNTVSGTINRTGTS
                     GAFYLYYNANGNGTGTENISGNSFSNITLAGTSSFSGIYSTINANGTQNIFNNTISGI
                     TGGTGTFHGMYLGTANTRSVYGNQIYNNTTGGVMNGIFVNSGNPSNIYKNEIYNLTSN
                     STATDYTMNGILVQAGTNVFIYNNFISDLKTPSSASFEAIRGISITPGQSNSTIGIYY
                     NTIYLNATSTGANFGTAGLYHVSSTDANFTRLDLRNNLIINNSTPNGSGTTVAFRRSA
                     QSTANYAATSNNNILYAGTPASNRLIYWGNTTKQTIEEYREFMGPNRDSISFSELPPF
                     INIATTPYNLRLQDGTTNYCESGAKPITTPLAITDDFDGAVRPGTPDIGADEFAGTSA
                     YVEFPAFLTATSVNSQQIRLEFDSNPDEDDVVIVFNTSGAFSLPAGTPVVGQPLAGGT
                     VVSVGTTSPVIHSGRTPNTAVYYKAYCYNGANYSLGKIANATPVVTPVTNLTAVCAGQ
                     SEIDLGWTKNIYDHDVMVAAHSVYMNGIPVNGTNYNVGDPIPVGGTVIYKGPASGFDH
                     TGLSSWTQFYYKAWSVDIFNYYSSAVTANAITDADPVTEYGYEQDFDGTWAHDVLAPP
                     NWLVVDVGGSGGFTWIRNSLAERSTPYCVQGYGSGACNDYLISPPLQLPDTLTQLTWW
                     DKVSVGTNINNYKVLLSTTNNLVSSFTVELGDYTCVNTAWQQHTLNLTPYRGQTVYIA
                     FYHYYTTQQYEYFVIDDIVIEKNVPGPASIGFPKDEMLTLVDQTLRWNSPVASIPVLG
                     YKLYLDSAPNPTTLVYDGAATSFTPANLAYNTTYYWKVVPYNANGEAYNVPVWLFTTV
                     TSSQLAQSFEAPYFPPVSWEIPSTGWFLNYSNAYHGLQSAYRFTYPALRPKLITPLLE
                     VKAGDELEFYEGTSDKINNFMVICYSTDKSDWDTLGGILDVTRGGWGHRVFDLSALAG
                     GQYYFSFEVWCATNYNAYVYIDHVIGPDVVPILPWAATNPDPPDTASFLPVNLTLGWA
                     PQIWGGIPSGYKVYLDTDPNPTTLIYNGRAESYAVGPLQLNTTYYWKVVPYNTLGDAQ
                     NSVVWSFTTVPVGGVQIGRDTEGFWDLPVYTSYGYNYSQTIYLQSEIDISGKQISKIY
                     YQWNGEEEGTQYKDWVIYMGHTEKTEFTSVTDWVPLSQMTEVFNGEVIIPYNDYDPVW
                     IEIILETPFDYNNIDNLVIAIDENTEGYSWAYGAEFYCTEDPNDRSIQYYSDDDNPDP
                     ASPPDADNLVTAYPDIRILCETAATTPVYFCSPASKDFGTVGTFMSSEPKVFKIRNRG
                     VGTLTLTSTALGGTDAGQFQLTDTNSYPVALLAGEYISMEISFIPTEEGAQTANLLVS
                     HDAAGSPGIVPLGGTGFESRIKTFPFIETFEDNSLTQNQWSQLQETGTSSWTYATGAG
                     DGTITTAHEGLLNARFTDLGLGAVTKLVSPVFDLTGVSNPQLIFWYGQENWSGDQNEL
                     KIYYRTAYNQPWVEIFYDNTNRAEWTTDTLSLPNPSLSYQLAFEGIDNYGYANVLDDI
                     YVGPPVTRWTGTISTDWTDPANWTNGVPGVNNAVIINTELYDPVIDVAVTVYAVIVEP
                     GSFITITADGDLTVTGD"
     gene            complement(15661..15981)
                     /locus_tag="PKZ74_01135"
     CDS             complement(15661..15981)
                     /locus_tag="PKZ74_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91381.1"
                     /translation="MDDNEQLAGLINKDLGDPDEGLLIPVSGIDRSEIHQKLSGMIAY
                     LIERHFERLCQAMYRLDVSERKFHQVMNEKPLDEIPGALADLVIEREIQKIHTRRMYK
                     SGQL"
     gene            complement(15974..16159)
                     /locus_tag="PKZ74_01140"
     CDS             complement(15974..16159)
                     /locus_tag="PKZ74_01140"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HPM91382.1"
                     /translation="MPVPSPCKLICRYDSDGLCAGCRRTREEITRWISYSDHEKLEVY
                     KKITLRQQSVSNAQHHG"
     gene            complement(16237..17109)
                     /locus_tag="PKZ74_01145"
     CDS             complement(16237..17109)
                     /locus_tag="PKZ74_01145"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HPM91383.1"
                     /translation="MKIYLIPVLLLIFSTVSSGQDLMDMLGDDEANTTDYTYATFKAT
                     RIINGQSIELPPKGALNFVISHHFGRINQGAYEFFGLDQANIRFGFEYGFTDWLSAGI
                     GRTSVNKTYDGSLKAKILRQSSGKRSMPITLTWFSNATISSLKWQEPDRKNYFTSRME
                     FAYQLLIARKFGSRVSLQLMPTYVHRNLVETIEDENDVFAVGAGGRIKITNRMAVTGE
                     YYYVLPGKTADDFHNSFSIGLDLETGGHVFQVYLTNSQGLIEEQFIPRTSGNWGDGDI
                     HIGFNINRTFQIVK"
     gene            complement(17146..17589)
                     /locus_tag="PKZ74_01150"
     CDS             complement(17146..17589)
                     /locus_tag="PKZ74_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91384.1"
                     /translation="MKKVLLIIIPVVVLLGAGGYFGYSYFFAEKPVAEVVLGPAEVKV
                     NAKRLWVDYSMNPDIAGPRYTGKIAEVTGSIMRVEAVDDEIVVVFAFKKGDAGDEGIR
                     VTMLPSFRQAARGINPFKNVTIKGVIKEYDRQNIIMEHGSIIGKQ"
     gene            complement(17620..17997)
                     /locus_tag="PKZ74_01155"
     CDS             complement(17620..17997)
                     /locus_tag="PKZ74_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91385.1"
                     /translation="MKNLIFLAILAIMAGCYYDNEEELYPGPGGDCDTTNVTFSGTIF
                     PMIDANCTGCHSGSAPQGNVKLEDYASIAAAAAKPAGQYGSLYGAISHASGNSPMPKN
                     GTKFSDCRIKQVKAWIDAGRPNN"
     gene            complement(18070..19074)
                     /locus_tag="PKZ74_01160"
     CDS             complement(18070..19074)
                     /locus_tag="PKZ74_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91386.1"
                     /translation="MRKLSFIILGVITALAVTYTGCKHEPEEYIPDPDPNPDTTSCDT
                     ASVTYPGKIYPILDQYCISCHSGAGPSGGLDFTDYSQVAFVAENGALIGAIKHLSGYS
                     PMPQGGEQLDSCKIRLIEIWIRDTTFLPPPDTSHPCDPDTVYFEMDVLPILMSSCGKS
                     GCHDATAQDGVRLDSYEAVMTSNVIRPGNPNDSELYEKILETDPEKIMPPPPDSPLDP
                     AQMEIIRKWIAQGAQNLYCDAACDTVNVTFNGTIWPEIIQKHCFGCHNGPNASGGIHL
                     ENYSDVSAAANILPGQPGSLMGAITHAAGNSPMPQNQPQLPDCKISQVRNWISLGKPQ
                     "
     gene            complement(19259..19876)
                     /locus_tag="PKZ74_01165"
     CDS             complement(19259..19876)
                     /locus_tag="PKZ74_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91387.1"
                     /translation="MKKSVFMLMIMMAVSGIRLSGQDFTAVREAFARSFSYEKESKYE
                     EAADELMKIYSDDSYEINLRLGYLAYMQGNYTESMAFYGKSITLQPYAIEPRFGYILP
                     ASAAGNWSQVEEQYKKIIAVDPMNTQANYWLGIIYYNREDYGAAAKNFEKIVNLYPYD
                     YDATIMFAWTNFKMSNLREAKALFQKALLIRPGDESAMEGIGLIQ"
     gene            complement(19881..21230)
                     /locus_tag="PKZ74_01170"
     CDS             complement(19881..21230)
                     /locus_tag="PKZ74_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91388.1"
                     /translation="MGTSKRKLLLILLFLMPFAAVPQEQRLSFREADSTSYALYAAGE
                     WKQLTRYCNRAFADSIDYYYLRMRAGIAAFETERYMRAALHFGKALEFNAKDPVAGEY
                     LYGCYLRLNRPDEARTILEDLPPSVREKLEPAMPYFHRAILETGPLMSNEMTRYDTVD
                     LDGPDDLYGEADITLDGYYLNAGVARAFKGGYNVYLGYTLVKLNKNKIAETGDSLSVD
                     DQYPLYQHQYYASGIIPVRKNYSVQAAFHYISNSYETVMPRLAADSVSYLYPRQAYTN
                     SSWIGYLSVTRDLKILKATLFGAISSMNDVNQYQGGFQLTAFPFGNLNLYTSSKLIDH
                     INDNNHNFIFEQMAGVRLFRPLWLEASATFGNMENYHENSGFVVYNIADKMTFKGNAR
                     LIYAFHPRWTATAGYSFLVRESSYVQYLLTDDLKVERMVKTEDFQSQVILLGLDFNF"
     gene            complement(21200..23416)
                     /locus_tag="PKZ74_01175"
     CDS             complement(21200..23416)
                     /locus_tag="PKZ74_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPM91389.1"
                     /translation="MSSERSKYLTYYQGITNSYALVFFSNNQILAWILIAVTFFDFIS
                     GLAGLISIMIANTVAWLAGLNVDKIRKGYYGFNALLVGLGLGLFFSATGEFFLVLVFS
                     ALLTLFITVALEGIIGKYNLPYLSLPFLFAIWLTFMASRDFFALQISNRGIYSMNEMY
                     ILGGMPMVRIWEWFNNLQLPQSLVIYLRSLGAIFFQYHLFPGLLIAVGLLIYSRIAFS
                     LSLAGFYAAYIFYQVVGADMTTLSYTFIGFNFILTAIAIGGFFIIPSWYSFLWIILLT
                     PITAILLTAFKMLFTFNQLSIYSLPFNIIVIVFLYVLKFRERHFDKPELVLYQRFSPE
                     NNLYSQRNNKLRFGRLPWFRVALPFWGEWCVTQAHNGSITHKDNWRHAWDFEITDEKG
                     KSYRNEGDRVSDYYCYNKPVLAPADGWIEEVLDGIDDNTVGEVNLDQNWGNTVVIRHS
                     EHLYSAVSHLKKDSLKGSAGDFVKKGDIIGLVGNSGRSPVPHLHFQFQATPQIGSATL
                     DLPLQHYILKTEEGFVLRSSDYPRKDECVSNIENNDLLRKSYNFIPGQEIIFRNEKME
                     KITEREVRWEVKADYLNNTFLYCSVTGSRAFFVNESELFYFTHFEGDKKSLLYHFYLG
                     SYKVIYGFYRGLKVKDHFPLNVLASGAMRYLQDFVAPFWLFASSVYRLDYTRSADPLS
                     DPELHLHSEAFVRLPFKTDRKLAFDICFRDDRLEKFIIFDEQKPPSEWVRQKENFS"
     gene            complement(23406..24767)
                     /locus_tag="PKZ74_01180"
     CDS             complement(23406..24767)
                     /locus_tag="PKZ74_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91390.1"
                     /translation="MGQKQRYERPAVKRLETHMPNKFGLRTAYVPVTHIDGVAVKDLI
                     KEHGSPLFVISEQSIREKYREAKQAFTTRYPKVQFAWSYKTNYLNAVCSIFHQEGSWA
                     EVVSGFEYDKAIANGVDSRYIIFNGPDKHEDDLRKAILNDSLIHIDHLEELYSIINLS
                     TELRKKPRVAIRVNMDTGVFPIWDRFGFNYENGQAWDALNKIMRSGKLELAGLHCHIG
                     TFMLTASAYATAAYKLADLAIGIHKRYNHEIKYIDLGGGFPSGNTLKGSFLPGADTNP
                     GFDVMAEAITSSLVNAAFPAGKMPLLILETGRALVDEAGYLLGTVISNKRSSTGKRIT
                     IIDVGVNLLFTSFWYDHRISPAQPFSNYTEETMLYGPLCMNIDVVRENAVLPLLNKGD
                     HFVIHHVGAYTMTQWMQFITLRPRVVLIDTDGRTHIIRERETLEIINRNESVPEHLKK
                     FEL"
BASE COUNT         6108 a         6171 c         6417 g         6304 t
ORIGIN      
        1 gaacgggttg ctcatgtcat gctccatgat gaagtcgtgc aggacatacc agtcgtcgag
       61 tgcctgctgg ccgccgtact cggcccaggt cccgccccag agcatgataa actggatatc
      121 gtattttccc tggtcgtagt aatattccgt ataatcatgg tcgtgcacct tttcgcgtaa
      181 ggcgtagatt ccccagtact gcccgttggc ataaatgaca acccgctccg atttccgcca
      241 gtcgagatgc tgacccgatt tttgagaaag ggtctggacg aaatcatcgc ggagatgggc
      301 gctggtatca atcccgggat aattgtcgtc gccggcggca cgaatgatca cccgctggaa
      361 ttcatcgcgg tctgacaagg agaagaattt ctgctgaagg gcataattat aaccggcttc
      421 atcgcgggtg atataatcga tgctcctctg tggatggacc cacgaatcct gcccgtgttc
      481 gttaaactcg ccataagccg tggtagtcct ctgtttcagc ttgttgtaaa attcgatgga
      541 tccccttggc cgcaatgctg aatttccgtt gagcaactcc agcagttcat ttcctgcaat
      601 ggaaagaacg ggcagagaat ggttggcgtt gataaaatat gtgttgaatt cgatcaggcc
      661 gggaagaatg gagggatcgt tactgaaaat cctggctttg agtatcttgg ttgaaaaaat
      721 tgaaatgggg gccgtgtatt cggtagcata ggagtatggt tccgttccgt cgagcgtata
      781 ccggattttg gagttgggtt cgggattcat gagggtaata gtgaccggat tgctatagaa
      841 tccgcccgta tcactgacca caggcttttt ggcgtatgcc gaataggctg tggagttgcc
      901 attggaggag cccggtgtag gggtggaaaa gatagcccac tgatcgcccc cgctgacggc
      961 acgtccgcgg gaatgatctt tttgtgtaat agcgagctgc acctggtcga tgatcacgcc
     1021 atccggatcg gagaggacga caaagtcggg cgaagatttt gtctgggtga ggcggaagct
     1081 ggcgtggtaa tggtttccgg agacttcgtt ccggcccgac gcccagatct tcaggtaacc
     1141 gttcgacgga aggaatacgc cggccgggaa ggtccataag gcagggctgg ccgtatcgtc
     1201 gctcaggtga tacccgccga tattgacgcc ggaagggccg gcattgtaca actcgatcca
     1261 gtcttcatac tcatcgtagt tatcctggat aaagctcagg ttggaggcgg agtattcgtt
     1321 gatgaggatt tgggataatg caggacatga gtggatcaac agcgctgcaa tagccggtaa
     1381 tactaacttt cgaaggagat tcatatgggc aggtttaagg ggtgatcaga ttctgatgac
     1441 ctgctaaatt actaataagt caaattccag ataaaataat ttccggaaat tcattttaaa
     1501 tgcaaaagtc attaaagtgg taaagttttt gataagaact tatcaaaatc atttcatttt
     1561 aagccgcagg tgatagtgtg gcggaagata gaagggagga tatcatgtac agggcggtca
     1621 aatacaatcc ggcaatacat catcgcaggt ccctcagatt aaaggggtat gattattccc
     1681 gggcaggtaa gtattttatt accatttgtt gtcaggacca taaatttcga ttcggaataa
     1741 tccaaaacca tacgatggta ttaaaccaat ttggcaggat tgcccaaaac gaatggtata
     1801 aattaccaga ccggttctac aatttgcaat tgggtgcatt ccaggtcatg ccaaaccata
     1861 tccacgcaat tattgttttg cacgaccaca ccgaaaacga taccgcagca ggggcaaccc
     1921 tcgcggttgc ccctgctgcg gttggaccga tcgataatga tccggttgcc ccggttgacg
     1981 acgtttaccc ggttgccccg atcgacgttt acccggttgc cccggttgac gacgtttacc
     2041 cggttgcccc ggttgacgat gtttacccgg ttgccacgga taataatttt gcaacagcag
     2101 gggcaggggc gagccctgcc cctgctgtat caccgtcaaa tccaacggtc ggaaatataa
     2161 ttggggcata taaatcgttg gttgccaacg aatgtttaaa aatattcaaa tcgaaaaacg
     2221 aaaaaatggg taaattgtgg caacggaatt attacgaacg gatcatccgg aacgatcggg
     2281 cgttcaatgc gatttccaac tatatcctga ataatccggc caaatggaat gcgaaaaaat
     2341 caatttcatg atctgatttt atcattccca attccccgat cggctaattc cggctttgag
     2401 acctgggggc aattgctttc aaacagactg gtgagggtgt cgcgaatatg gaatagtcgt
     2461 cgatgataac cgttcgaagc atccattttc ccttattctt caaaaatacc taaattcaag
     2521 tcttttagca agaataattt acagtttcaa cggaagattc ttcaccaatc ctataaccga
     2581 cgtgtaaatc catcggaatg cgaacatcag aatgaaccct gttttccggc aaaaaatcgc
     2641 cgatcacctc aaattactta tatcaatcta tattcaaatt acatttacag ccgaaattct
     2701 gccagagatg aagatcaaac ctgcatcagt tctgttccat gttatcggat gcacgatctt
     2761 cctgctgctg ccgatcattt tctggccggg agaagggggg atcagcgact tcttcaatga
     2821 ttcccgggct atccgtggat attccgtcaa catcctggtc ctgatctttt tctatcttaa
     2881 tttctattat ttcattccac gtttttattt taaaaaaaga tatgcggtat tcattctgat
     2941 cctgttcgct ttttacctgg tcattatttt ccttcctgaa ttaattgttc catttaatga
     3001 gcctccgcgg cgtatgccac ccggtccgga accgggcgga aaacacctga tcggcagcgg
     3061 taaatggttc ttccggatgg gtcatcacct ggtattcttt ctggccgtcg tctttttctc
     3121 tcttctgatc aaaatccaca accggctgaa attgaccgaa caggagaaac tccaggccga
     3181 actgtcctat ttcaaggccc agatcaatcc ccatttcctt tttaacacac tgaactcgat
     3241 ctactcgctg gctatccaga aggccgacaa cacgcccggc gccgtcgtca aactatcggg
     3301 aatgatgcgc tatgttctat cggaggccag taaggagatg gtgccgctcg accgggagat
     3361 cgggtatatt accgattata tcgagttgca aagaatccgc ttcggggaaa cggttaaaat
     3421 cgattatcat ccctgtgaaa ccgctccggg aaaagttatt gcgccgcttc tgctgatccc
     3481 gttcatcgaa aacgccttca aatatggcgt caacccggaa gctgattcca atatcactgt
     3541 cagggttatt ctctatgaaa acgatcttca cctgcatgtt ttcaattaca aagtccagga
     3601 ccggaaagag gatgaagttc ccgggggagt aggtgtgaac aatgcccgta aaaggctggg
     3661 gctgatctat ccgggcaaac acaagctgga tatcaacgaa aatgaatttg agtataaagt
     3721 tgatctatat attgacctgg gatgataact gccattgcac tcgatgatga gcctcccgca
     3781 ctgaggatcg tggaaaactt ctgcggaaag gttgatttta tccgcctgga gaagagcttt
     3841 acccatcctc acgaggcgtt gaagcacctg gagaagttcc cggccgacct gctctttctc
     3901 gatatccgga tgccctccat gtcgggcgtg gaatttgcta aaaaagtgcc cggagaactg
     3961 atggtcattt ttaccactgc gtacagtgag tatgccgttg aaggtttcaa cctcaatgcg
     4021 gtcgattatc tgctcaagcc ctttacattc gagcgtttca tgcaggcagt gaacaaagcc
     4081 ggagactatt accggttcct tcaccagtcg gaacaggccc tgcagcaata tctctttgtc
     4141 agggccgatt acagcctggt caggatcata ctggatgaga ttctgtatat tgaaggattg
     4201 gatgattacc tgaggatctt cctcacgaac cgcaagccgg tcgttgcccg gatgacgatg
     4261 aaaacgatgc tggagaaact gcccgccgac cggttcgcca gggtgcaccg gtcctatatc
     4321 gtccccgttt ccaggatcga taaggtgagg aacaaaatca tttttatcgg agataagcag
     4381 ataccgatcg gaaatagcta tgaaaagggg attttcaggg aatagttaaa aaggaaatgg
     4441 gaaaagggaa aaataatcaa atcgtaaaga aattaataat aatttaactt tgccccagtt
     4501 ttaatattca ggatgcgcat ttcccgccgt catattctca tcattctctt tgtgctgatc
     4561 gtcatcacca tctttctccc tattccggtc aggtacagtt ttattgccac ggcaaagatt
     4621 tacccgttca gggaatggaa actgatgcgc ggaacggaag agggattctg gagccagact
     4681 ttcaattacg agaccgacgc cctgggcgat ttcaagaatt accggtttga gcggggtgat
     4741 attgccgaac tggagataaa tgagaagctg gttttcgata tgcctgtgag ccagcatgat
     4801 accatcgcac atatccgttc tttctttatt gaaaatgaga tcacccggct tgaaaacctt
     4861 cgcgatgttg agaccgcaaa caaggatgtg gtgtcgaccg gcgagaagcg accgctggtg
     4921 gagcaggcac agcgccaata caactacgct ttgcagcagc tcgacctgga gaagaagaat
     4981 tttgcccggc aggaaaagct tttccgcgac agcgtgatca ctcccgcgga gttcgacttg
     5041 tatgaaaaca ccctgaaact ggccgagatc aatgcacagg tagcttttga agccctgaca
     5101 tcgctgcaga ccggggaaaa agacccggtc gtaaacctgt cggaacagaa gatcacctca
     5161 tacgaaaaag aggttgaacg tttgaaaaca cagaaagcgc aatacacgat cgttaccccc
     5221 atatccggca tcctgagcta caatacggag atcggcggga tcataaaggt gagcgacagc
     5281 gaaaagctct tgttgaagat cccggttgca tacgaacact cttcttatct gaacaacctt
     5341 tacaaagtca ctttttcgac tcccgacaat aaaataacgg tcaatgcctc ctttaagggc
     5401 ttcgacgaga gcgtgaacca gatccagaac aagcagtttg tgatagcccg tgcggtgacg
     5461 accgggccaa cgacagggat ttacccgggg atggtggtgc aatgccgtat ttattgcgat
     5521 aaagtttacc tgctggagta cctcaaacgg aatttttccg tatctttctg atcatgaagt
     5581 acgagtttaa atatgtggtg ccggtgagcc agctcgatgc gctcagggag gcgataaagc
     5641 cttacgtgga ggtggatccg tatgccgaag caacccatct taacgagtac acggtccgat
     5701 cgatttatta tgatacggcc aggttcgatt atttttttga aaaggtcgac ggatataaaa
     5761 tgaggaagaa gatccggatc agggggtata atgatcaaaa aggcggagat acggtcttta
     5821 tggagatcaa aaggaaattc aaggagccga tcgagaagga ccgggagaag ctgacctttg
     5881 aagtcatgaa acgcctgatg gcgggcgaag gagcggctgc ttacggcgat gtgcccgatg
     5941 atcacggggt caacggggcc ggcaagtttc tctatcatgt ttaccgtaac aacctgaagc
     6001 cggtggtgct ggtgatttac gagcgggaac cgtatttcga ccggtttaaa accgatgtca
     6061 ggataaccat tgataaaaac ctgcggagca tcgcctaccc taccctcgat gatctttatt
     6121 ctgagaataa agtgatcccg gctatcccgg gcaggttcat ttttgaggtt aagttcaaag
     6181 atcagttccc ggtatggctg cggccgatca ctggccacct ggggctggtc agaaggtcgg
     6241 catcgaagta tgttttctgc atcgattccc atcgcctgac ggagccgtca aaaaaatcgg
     6301 atatgttcag gtttatgcac tggaacaggt tataattaca ggaagatgtt agaagaattc
     6361 accaacatag agctattccc gctgaacgtt tacacggtgc tcaccaacat gggcgtttcg
     6421 ctggcgtgcg ggatcgtcat tgccctggtt taccgcttta tttacaaagg gccgagctat
     6481 tcggccacct atgtgaactc gctggttctc ctgagcctta tcaccacgat cgtgatcatg
     6541 gtgatcggca acaacctggc gcgtgctttc gggctggtcg gcgccatgtc gatcatccgg
     6601 ttccggacgg cggtgcggga tgtgcaggac atcgtctata tcttctttgc gctaacggta
     6661 gggatggcct cgggtgtggg gctttatgcc atcgctatct cgggaacgat cctgatctgc
     6721 ctggtttccg tggttatggt tacgtttaac ttcggcgcac ccggccgggc ggagtatgtc
     6781 ctccagatca tctacaaacc atcgaaagat accgaggacc ggatcgcagg catcctgcag
     6841 aaatactgcc gggggatccg gctttctaac ctgaagagcc agggtgagaa cgacggcatc
     6901 gaagcgtttt accagttcag cctgcgacgg aagtacaaaa gcacggagct cgtggaggag
     6961 ttctcgcacc tttcggaaaa ggtaaaattc aatctctact tcgaccagga cgaggcgaat
     7021 atcgcttttt agttccaggt tccaggttcc aggttccagg ttccaggttc caaatttcca
     7081 aattgcctaa tttccaaatt tccaaatttc caaattgcct aatttctttt atctttgcag
     7141 ttcatatcta aaaactaacc tatgaaagca aaattctttt tctttttcat ccttttttta
     7201 ggattcagtg cgatcacttt tgcccaggca acaacggccg atccggtgga aaaaaaggat
     7261 gcacctgcgg tggtaaccag ccctgatcat gcaaagaccg gagaatgcac cgggcatacg
     7321 acaacggcga aggccgactg caaatgggtt gacgccaatg gcgacggcaa atgcgacagt
     7381 tgcggcaaga ccgaaaagga gtgcaaggaa gcgtgtgccc ccaaggcggc tgccaccacc
     7441 ccgaaatcgg gttgcgcatc aacctgcccc catgccaaag agtgcggtaa atcgaccgga
     7501 acgcttccca agaaggacta aggctgattt atttctgccg gagccagact gtcatctaca
     7561 gataatccgt gcaaatccgt ttaattcgtg tcgttcgcgt tctataatgg aacacgtata
     7621 aacacggatg ttacggattc acacggattt gatttataca tgatgatcct ttttgcgttt
     7681 cagtcacccg taacggtaag atcaccatct gccgttatcg ttatgaatga gccgggttct
     7741 acgattacag cataaacagt caccgcaaca tcgataaccg gatcatataa ttccgtatta
     7801 atgatcacgg cattgttcac ccccggaacg ccgttggtcc agttcgccgg atcggtccag
     7861 tcggtcgaaa ttgtaccggt ccaccgggta accggcggcc cgacatagat atcatcgagc
     7921 acattggcat agccgtaatt atcgatccct tcgaaagcca gttgatagga taatgaagga
     7981 ttcggcagcg aaagtgtatc ggttgtccat tcggccctgt tcgtgttgtc ataaaaaatt
     8041 tccacccagg gttggttata ggctgtccgg taatagatct tcagctcgtt ctgatcgccg
     8101 ctccagtttt cctgaccata ccagaaaata agctgagggt tggaaacccc tgtcaggtca
     8161 aacaccgggc tgaccagctt ggtgacggcg ccaaggccca gatcggtaaa ccgggcattc
     8221 aacaaccctt cgtgcgcagt ggtaatggtg ccatccccgg cgcctgtagc gtaagtccac
     8281 gaactggttc ccgtttcctg aagttgcgac cattggttct gtgtcagcga attgtcttca
     8341 aatgtttcaa taaaggggaa tgtcttgatc cggctttcga aaccggtgcc gccgagtggc
     8401 acaattcccg gagagccggc cgcatcgtgc gaaactaaaa ggtttgcggt ctgtgcgccc
     8461 tcttcagtgg ggataaaact gatttccatc gaaatatatt cgccggcaag caaggcaacc
     8521 ggatacgaat tagtgtctgt cagctggaac tgaccggcat ctgttccacc cagagcagta
     8581 ctggtaagag ttaatgttcc tacaccccgg ttcctgatct taaatacttt cggctcagaa
     8641 gacataaacg ttccgaccgt gccaaaatcc ttcgatgccg ggctgcagaa atagaccggt
     8701 gtagtcgctg ccgtctcaca caggatgcgt atatcgggat acgccgtcac caggttatcg
     8761 gcatcgggcg gactggcggg atcgggattg tcgtcatcgc tgtaatactg gatgctcctg
     8821 tcatttgggt cttccgtaca ataaaattca gcgccgtaag cccatgagta cccttccgta
     8881 ttttcatcga tcgcgatgac caggttatcg atattattat aatcaaaggg ggtttccagg
     8941 atgatttcga tccaaaccgg gtcataatcg ttataaggaa taatgacttc tccattaaaa
     9001 acctctgtca tctgtgacaa aggaacccag tcggtaacag aagtaaattc cgttttttcc
     9061 gtgtggccca tatagatcac ccagtcctta tattgggttc cctcttcttc accgttccac
     9121 tggtaataaa tttttgagat ctgcttgccg gagatatcga tctccgattg caggtaaatg
     9181 gtctgggaat aattatagcc ataggaagta taaacgggca ggtcccagaa tccctcggta
     9241 tcccttccga tctgaacgcc cccgacaggc accgttgtaa acgaccatac cacactgttc
     9301 tgagcatctc ccagtgtgtt atacggcacc accttccagt aataggttgt atttagctga
     9361 agtggaccga cagcatatga ctccgccctc ccattgtaga tcagtgttgt cggattggga
     9421 tcagtatcga ggtagacttt atagcccgac gggatgccgc cccagatttg cggggcccag
     9481 cccagtgtca ggttaacagg caggaaggac gcagtatccg gcggatccgg gttggttgcc
     9541 gcccagggta aaatgggaac cacatccggc ccgatcacat ggtcgatata aacataggca
     9601 ttatagttgg ttgcgcacca cacttcaaag gagaagtagt actgaccgcc ggccagggcg
     9661 ctgagatcga atacccggtg gccccaacca ccccgggtaa catccaggat gcctccaaga
     9721 gtatcccagt cggatttgtc agtggaatag caaatcacca taaaattgtt gatcttgtcg
     9781 gaagttccct cgtaaaattc aagctcgtcg ccggccttga cctccagcag cggggtgatg
     9841 agtttcgggc ggagtgcagg ataggtaaac ctgtatgccg attgcaatcc atgataggca
     9901 tttgaataat tgaggaacca tcccgtgctc gggatttccc agcttaccgg aggaaaatag
     9961 ggcgcctcaa agctctgtgc aagttgagag gaggtcaccg tcgtaaataa ccagaccggc
    10021 acattgtatg cttctccatt ggcattgtaa ggtacgacct tccagtaata ggtcgtattg
    10081 taagccagat tggctggtgt aaagctggtt gccgcgccgt cgtaaacgag tgtcgtcggg
    10141 tttggggcac tatccaaata gagcttataa cccaaaaccg ggatggatgc caccgggctg
    10201 ttccatctca gggtctgatc gaccagggtc agcatttcat ctttcggaaa accaattgaa
    10261 gccggacctg ggacattctt ttcgataact atatcgtcga tcacaaaata ttcatattgc
    10321 tgtgtggtat aataatgata aaaggcaata tacacggtct gtccccggta aggcgtgagg
    10381 ttaagcgtat gctgctgcca tgctgtgttc acacaggtat aatcacccaa ttctaccgta
    10441 aaggatgaaa caaggttatt ggtcgtggaa agcaacactt tgtaattatt aatatttgtc
    10501 ccaaccgaaa ctttatccca ccaggtaagt tgagtgaggg tatcgggcaa ttgcaatgga
    10561 ggtgagataa gataatcgtt gcaggccccg cttccataac cctgcacaca atacggtgta
    10621 gatctttccg ccaggctgtt cctgatccag gtaaagcccc ctgaaccgcc gacatcaacc
    10681 acaagccagt tgggaggcgc cagcacgtcg tgggcccagg tgccgtcaaa gtcctgctca
    10741 tagccatatt cggtcaccgg atcggcatcg gtgatcgcat tggcggtcac cgccgacgaa
    10801 taatagttga atatatcgac cgaccaggcc ttgtaataaa actgggtcca tgagctcaag
    10861 ccggtatggt caaaaccgct ggccgggcct ttgtagatta cggttcctcc gaccgggatc
    10921 ggatctccca cattgtaatt cgttccgttg accggaattc cgttcatgta aacgctgtgc
    10981 gctgccacca ttacatcgtg atcatagatg tttttcgtcc agccgaggtc aatctccgac
    11041 tggccggcac agactgcggt gagattggta accggcgtaa cgaccggagt cgcgttggca
    11101 atttttccaa gagaataatt ggcgccattg taacaatagg ctttataata tactgccgta
    11161 ttcggagtcc ggccgctgtg gatgacggga gaggtggtgc cgaccgaaac gacagttcca
    11221 ccagccagcg gctgcccgac aacaggagtg ccggccggaa gtgagaatgc gcccgaagtg
    11281 ttaaaaacga taacgacatc atcctcatcc gggttggaat caaactcaag cctgatctgc
    11341 tgcgaattaa cggaggtagc cgttaagaag gcgggaaatt ccacataggc cgatgtaccg
    11401 gcgaattcat cggcgccaat atcaggcgtt ccgggcctca ccgctccgtc gaaatcatcg
    11461 gtgatagcca ggggcgtggt tataggctta gcgccgcttt cacaatagtt ggtcgtgccg
    11521 tcctgcaggc gaagattgta gggggttgtg gcgatattga tgaacggagg aagctcgcta
    11581 aacgagatgg agtctcgatt ggggcccatg aattcgcggt actcctcgat ggtctgtttg
    11641 gtcgtattcc cccagtatat cagccggttc gacgccgggg tgccggcgta tagtatattg
    11701 ttgttcgatg ttgccgcata attggccgtg gattgggcgc ttcttcggaa tgccacggta
    11761 gtaccgctgc catttggcgt agagttattg atgatgaggt tgttgcggag atccagcctg
    11821 gtaaaatttg catcggtact ggagacatgg taaagaccgg ccgtgccgaa attggcccct
    11881 gtcgatgtag cattcagata aatagtattg tagtaaatgc caatggtaga gttgctctgt
    11941 ccgggggtga tggaaattcc tctgatcgct tcgaaactgg cggatgaagg ggtctttaaa
    12001 tcggatataa aattattgta aatgaacaca ttggtgccgg cctggaccag gatgccgttc
    12061 atagtatagt cggttgcggt cgagtttgat gttagattat aaatctcatt tttataaata
    12121 tttgaaggat tccccgaatt cacaaagatc ccgttcatca cccccccggt ggtgttgtta
    12181 taaatctggt taccgtaaac cgaccgggta ttggccgttc ccaggtacat gccatggaaa
    12241 gtgcccgtcc cgcctgtgat gccggatatc gtgttgttga atatgttctg ggtcccattg
    12301 gcgttgatgg tagagtagat cccgctgaaa gatgaggtgc cggcaagggt aatatttgaa
    12361 aaactatttc cggagatatt ttcagtgcct gttccatttc cgtttgcatt gtaataaaga
    12421 taaaatgcac ctgaggtacc ggtccggttg atcgttccgg aaacggtatt gttctggacg
    12481 ctggtaaccg aagggttgag ccgctggtta tggttataga tcagatgagt gcttccggtg
    12541 gtttgaatgc tgcttgccgc gaaagtgttg ttatttatgg tgatgttgct tgaagatact
    12601 attgacgact ggaaattatg gataaaggaa taaacagcgg tagatgaagc ggtattcgcc
    12661 agcgcaatgt tgttgtaatt cagttgcagc gggccataga cggcattgta gatgccatat
    12721 aggatcgagg aagcggaagt cagattgatg ttattgtatt cggccgtaaa cgagaccccg
    12781 tcggtgctgg catgatgaat acccgaagcg gtggccgaaa aagcggttcc gccgccggtc
    12841 atattattga tggtattgta ttggatgagg gaattggtgt tttttgtaag ataaattccc
    12901 caggtatcaa aggcggcatt gcctccgaaa ttggtgatcg tgttgccaaa gccgctgaca
    12961 ccgacagtaa aatcgtggtc attgtaatca tccgaacctt tcatgtaaat cccggcaaat
    13021 acattgctga tcgtattgcc cgtaagggtg atgttctcat gcgctccccc cggactggtc
    13081 acgcccacat catttgttgt ggaggtattg ttccccacac agatgcccgc cacgtatcgg
    13141 ctcgcccctt tcgtcagggt gatggttgaa ttcctgatcg tcacgttttt gcaggcattg
    13201 gtcaccgatt ccttccggag ataatagccg tattcgatcc cgctgttgga agccgtcacg
    13261 tcgatactgt cgaaggtcac gtaatcggtc ccttgcaaca tgactaccgc atcgccgttg
    13321 ttgcctattg tggaagtcga gaccgaacca acatcggtcc gggtgatcag cggtttggcg
    13381 ccggctccat atttccggaa cgtgattgga ttggaggcag tcccggttgt atagagccac
    13441 accggcgccg tgacgctctc gctatgcccg ccggccacgt tgaagatcac cccgccgggt
    13501 ccgatcaccc cgctgttgag cgcggtgact gccgcggcaa tcgttgcgta atcgcccggg
    13561 atggccttgg tgccggtgaa ggttgcgcca atggtcctgc tccctgttgg tgcagtaatc
    13621 gtcggtgttt ttcctgatcc ggtttctgaa gtgacgaaac ctgtacatgc accatcaact
    13681 gtttgtccgg ctgtagctgt ggatttgata tcataggcca gccaaaaata attgacaccc
    13741 ggggcaagta cctggctgcc attcatggta aaaccaccgg atggattatt ttgtgttcca
    13801 aaaagagtgc ttgttgaaaa aataggtgta gtagtataat atatctttac agtattaaca
    13861 taaatatcac cattaaccgt cacactgatc gagttcatat acaacggatt ggcgctcttc
    13921 tgcgtaacca cctgcaagcg gatgatgtcg ttgtcggtcg ttccgatgcc gacattggtc
    13981 gtattgggct gctcggtcgt tgaagtcgaa tacgacatat tcaacccatc ctcaaacaaa
    14041 acattatcca tatcaaaata ataatctccc gatccccggg tacataaaaa cctgatctta
    14101 ataaaatcgc cgctgtatgc agccaggctc accgccgtac tggtaaaatc ggttgtggcg
    14161 gtatgattat tctcatctat ggtataaatg gtcgtaaatg tagccccgtc gtcggtagag
    14221 acctggatat cgactttatc gtccggccca agctcggtgg cattcagcgg gaaccccagg
    14281 taattgacaa agcggtaatg aaagctcagg taagtgtctg atgtaatgct tccgaccagc
    14341 ggtgaaatgg catagcagct tgtattgctt gaggtgagcc gttcatactg ccctatgctc
    14401 aaagaggttc cgtggttggc cgctctcgac atattgttcg accattccgc cgggcttgtg
    14461 gttgagggaa aagtttgtaa atacggcaat gcatatcctg tccgggtact ggccgttaca
    14521 ccgggtgaat aatcgggaac agcatcatac gaccatatct tgtaatagta caccgtgttc
    14581 gggctcaggc tggtatgcga aaaactggtc ccgttgccgt aaaacaatac ggtccctgtt
    14641 ccttctaccg taccaccggg agtataaacg gtcccggtta ccggggttcc gaaagaattg
    14701 gcagtattgt atgccagcat cacattatgg cccgaccagt tcttcaccca gtccaggtcg
    14761 atctgggtca ggcttacggc ttcagccgtg aaagctgtcg gattgtacaa cggcttccag
    14821 gtataggtca gtccggctgt cggtaccgtg gccggagtgg cgctgttgaa atacatggta
    14881 ttcgagttgc cggtcccccg cgtggaattg atccagttgc ctccccccgc agcgatcgtc
    14941 cggttgtgaa cgttggtggc cagcgtattg tccggccctc tcagcccgac ctgcggatag
    15001 gtcgtattgg gccccggggt gatcgtccct ccataaacca ctttgatagt attattggaa
    15061 gtattaagcc tgacctggaa gctgatgatc tcaccggtta tattaattct cctgacatct
    15121 ttccattgga tgacaaattc gctccccacc tgctcatacc tgacggcggg cgttccgctg
    15181 gccgcctggt tcagatcgcg gccaaatgcc gaaatagctc cggaataggt tgcggtattg
    15241 ccaatgggat tataaactgt ccccgccggg gcgctcccga aagtaataaa cccattaaca
    15301 ctgatataaa tggctgtgta ggccgtaccg tcgtaagtaa acgaagggat ggtcaccgcc
    15361 ccggatactt cgttgtcaaa agatgtagcc cataattccg tcccaccgtt gatttcggta
    15421 taggtaccgt tctgttgtgt aaaatagtac tccgctacct gcgcattggt aaacaggctg
    15481 ataactaata agataagggt ataacttaac cggatcttca tggcgcttgc tgttttattt
    15541 tggttggatt aggacaaagc aataagatct cgtttttgag ttcttaaaaa taatgaaaat
    15601 tcaatgaaaa taaaaagaaa tatcttgaat tttcagtaat aacagggaac gcgataaagt
    15661 ctacaattgg ccgcttttat acatccggcg ggtatggatc ttttgaattt cgcgttcgat
    15721 gaccaggtcg gccagggcgc cgggaatctc atcgagcggc ttttcgttca tcacctggtg
    15781 gaatttgcgc tccgacacat cgagcctgta catggcctgg cagagccgct caaagtggcg
    15841 ctcgatcagg taagcgatca tcccggagag tttctgatga atttcgctcc ggtcgatgcc
    15901 ggagacaggg atcaacaggc cttcgtcagg atcgccgagg tctttgttga tcaatccggc
    15961 cagttgttcg ttatcatcca tgatgctgcg catttgatac actttgttgg cgcaaagtta
    16021 tctttttata gacttccagt ttttcgtggt cggaatagct gatccagcgg gtgatctctt
    16081 ctctggtccg cctgcacccg gcacaaaggc cgtcggagtc gtaccggcat atcagtttac
    16141 aaggacttgg aacgggcatt ttttttgttg ctaaacgatt gcatacataa aattgttcat
    16201 atgccggtaa atccttctat tagtagtccg acggctttat tttacaatct ggaatgtccg
    16261 gttgatattg aacccgatat gaatgtcgcc atcgccccag ttgccgctgg ttctgggaat
    16321 gaactgctct tcgatcagcc cctgcgagtt ggtcaggtaa acctggaaga catgcccgcc
    16381 ggtttccagg tccaggccga tggagaatga attgtggaaa tcatcggctg ttttgccggg
    16441 cagcacgtag taatattctc ccgttacggc catccggttg gtgatcttga tcctgccgcc
    16501 ggcgccaact gcgaaaacgt cgttctcgtc ttctatcgtc tccaccaggt tccggtggac
    16561 atacgtcggc atcagctgga gcgatacacg cgacccgaac ttgcgggcga tcagcagttg
    16621 ataggcaaac tccatgcggg aagtgaaata attcttccgg tcgggctcct gccatttcag
    16681 ggaagaaatg gtggcattgg agaaccaggt cagggtaatg ggcatgctcc gctttccgct
    16741 gctctgcctg agtatcttgg cctttagcga cccgtcgtag gtcttgttca ccgacgttct
    16801 cccgatgccg gccgaaagcc agtcggtaaa gccatattcg aacccaaacc ggatattggc
    16861 ctggtcgagc ccgaaaaatt catacgcccc ctggttgatg cgcccgaagt ggtgcgagat
    16921 gacaaaattc agcgcccctt tcggcggcag ttcgatcgac tgcccgttga tgatgcgcgt
    16981 cgccttgaat gtggcgtaag tgtaatcggt ggtattcgcc tcgtcgtcgc ccagcatatc
    17041 catcaggtcc tggccggatg aaacggttga aaagatcaat agtaaaacgg ggataaggta
    17101 gattttcatt ttcatggatg aacagcttgg tatgaataat tttatttatt gttttccgat
    17161 gatggagccg tgctccatga tgatattctg ccggtcgtat tctttgatca cccctttgat
    17221 ggtgacattt ttgaacgggt tgatccctct ggcagcctgg cggaaggatg gcagcatggt
    17281 cacccggata ccttcatcgc cggcatcgcc ttttttgaag gcaaaaacca ccacgatttc
    17341 gtcatccacg gcctccaccc gcatgatact gccggtcact tcagctattt tcccggtgta
    17401 cctgggcccg gcaatatcag ggttcatcga ataatcgacc cacagccgtt tcgcattgac
    17461 ctttacctcc gccggcccca gcaccacctc agcaaccggc ttttcagcga aaaagtagga
    17521 gtaaccaaaa tatccccctg cccccagcaa tacaaccaca ggaataatta tcagcagaac
    17581 tttcttcatt tcaactcaaa acttattatt aactcaatac tagttgttcg gtcttccggc
    17641 gtcaatccag gcctttacct gtttgatcct gcaatccgaa aacttcgtcc cgtttttagg
    17701 catcggcgag ttgcccgaag catggcttat ggcgccgtac aaactgccgt actgcccggc
    17761 cggtttggct gccgccgccg cgatcgatgc gtaatcctcc agcttcacat tgccctgcgg
    17821 cgccgaaccg ctgtggcagc cggtgcaatt ggcatctatc atcgggaaga tcgtccccga
    17881 aaaggtcaca ttcgtcgtat cgcaatcccc accgggacca ggataaagct cctcttcgtt
    17941 gtcgtaatag catccggcca ttatcgccag tatcgccagg aaaatcaaat ttttcatagt
    18001 tatgtcaatt tatcgttcca ggttccaggt tccaggttac accgttcact cttcacgctt
    18061 cacgcttcac tattgcggtt ttccgagact gatccagttc ctcacctgcg agattttgca
    18121 atcgggcaat tggggctggt tctggggcat cggtgaattg ccggcggcgt gcgtaatggc
    18181 ccccatcagg ctgccgggct gcccgggtaa aatatttgcg gcggccgaca catcgctgta
    18241 attctccagg tggatcccgc ccgaagcatt gggcccgttg tggcaaccga agcagtgctt
    18301 ctgaatgatc tccggccaga tggtcccgtt gaaggtgaca ttcactgtat cgcaggccgc
    18361 atcgcaataa aggttctgcg ccccctgggc tatccatttc cggatgattt ccatttgcgc
    18421 cggatccagc ggtgagtcgg gcggtggcgg catgatcttt tcgggatctg tctcgagaat
    18481 cttttcgtaa agctcgctgt cgttcggatt tcccggacgg atcacattgg atgtcattac
    18541 ggcttcataa ctatcgaggc ggactccatc ctgcgccgtg gcatcgtggc agcccgactt
    18601 tccgcacgac gacatcagga tcggcaatac atccatctcg aaatagaccg tgtcgggatc
    18661 acaggggtgg cttgtatcgg gcggaggcag gaaggtcgta tcccggatcc agatctcgat
    18721 cagcctgatc ttgcagctgt cgagctgttc tcccccctgc ggcatcggcg agtaccccga
    18781 aagatgcttt attgcgccta tcaaagcccc gttttcagcg acaaaagcca cctggctgta
    18841 atcggtgaaa tcgagccctc ccgatggacc ggcgccgctg tggcacgaaa tgcaatactg
    18901 gtccaggatc ggataaatct ttcccggata ggtcacgctg gcggtatcgc aactggtagt
    18961 atccgggttg ggatcgggat cgggaatgta ttcctccggc tcgtgcttgc atcctgtgta
    19021 agtaaccgcc agtgctgtga tcactccgag gataatgaac gataattttc tcatggcatt
    19081 caattgggag aaacaaaaat atacaaatcg acattaatct gtccccaaaa cacgctcaaa
    19141 aagaaattca acctgctaaa caggcaattt cagccgataa accggcaaat catcctccct
    19201 cagccctgcc ttcccccaaa tacactacca attaagaatt aaaaattaac ataaaacatt
    19261 actgaataag tccaattcct tccatggccg actcatcccc cggcctgatc aggagggcct
    19321 tctggaacag ggctttcgcc tcgcgaaggt tcgacatctt gaaatttgtc cacgcaaaca
    19381 tgatcgtggc atcatagtca tatggataca gattgacgat cttttcgaaa tttttcgcgg
    19441 cagcgccgta atcttcccgg ttgtaataaa tgatcccgag ccagtagttg gcctgcgtat
    19501 tcatcggatc gacggcaatg atctttttgt actgttcctc tacctggctc cagttgccgg
    19561 ccgccgatgc cggtaaaata taaccgaacc gcggctcgat agcatacggt tgcagggtga
    19621 tgctctttcc ataaaacgcc atcgattctg tgtaattccc ctgcatatat gccaggtatc
    19681 ccagccttaa attgatttca tacgaatcat cggaatagat tttcatcagt tcatccgctg
    19741 cctcttcata cttgctctct ttctcatacg aaaagctccg ggcaaatgct tccctgacag
    19801 ccgtaaaatc ctggcccgaa agccttatcc cggacacggc catcattatc atcagcataa
    19861 aaacactttt tttcatcttc ttaaaaatta aaatcaagac ctaacagtat gacctggctt
    19921 tgaaaatcct ccgtttttac catcctttca acttttaaat catcagttag caggtactgc
    19981 acatagctgc tttcccttac caggaaagaa taccctgccg tggccgtcca ccgcggatga
    20041 aaagcataga tgagccgggc attgcctttg aaagtcattt tatcggcaat attataaacg
    20101 acaaacccgc tgttttcatg gtagttctcc atattcccga acgtggcaga agcctccagc
    20161 cagagcggcc tgaagagcct cacgcccgcc atctgctcaa aaataaaatt gtggttgttg
    20221 tcattgatat gatcgatcag tttggaagag gtataaagat tcaggttgcc gaaggggaaa
    20281 gcggtgagct ggaatccgcc ctggtactgg ttaacatcgt tcatcgacga aatcgccccg
    20341 aacagtgtgg ctttcagaat cttcagatca cgggtcaccg aaagataacc gatccaggaa
    20401 ctgttcgtat aggcttggcg gggatagaga taacttaccg aatcggctgc caggcggggc
    20461 attaccgtct catagctgtt gctgatgtaa tggaatgcag cctgtaccga gtagtttttc
    20521 cttaccggga tgatgccgct ggcatagtac tggtgctggt agagcgggta ctggtcatcc
    20581 accgacagcg aatcgccggt ttcggctatc ttgttcttgt tcagcttcac cagggtatag
    20641 ccaagataca cattataacc gcctttgaac gcacgggcca cgccggcatt caaataatag
    20701 ccgtcgagcg taatgtccgc ctccccgtaa agatcatccg gcccgtcgag atccacggtg
    20761 tcgtaccgtg tcatttcgtt gctcatcaag gggccggttt cgaggatcgc ccggtgaaaa
    20821 taaggcatcg ccggctccag tttttccctg accgaagggg gtagatcttc aaggatggta
    20881 cgggcttcat cgggccggtt caaccggaga taacatccgt aaaggtattc tccggccacc
    20941 gggtctttcg cattgaactc aagcgccttg ccgaaatgga gggctgcccg catatagcgc
    21001 tccgtttcaa aagcggcgat accggcccgc atcctgaggt aataataatc gatgctgtcg
    21061 gcaaaagccc ggttgcagta acgggtgagt tgcttccact cgcccgcggc atatagggcg
    21121 taacttgtgc tgtcggcttc gcggaacgaa agccgctgct cctgcggaac agccgcaaaa
    21181 ggcataagaa atagcaggat caggagaagt tttctttttg acgtacccat tccgatggtg
    21241 gtttctgttc gtcaaagatg atgaattttt cgagcctgtc gtcacggaag caaatatcaa
    21301 atgccagttt ccggtcggtt ttaaatggta gcctgacaaa ggcttcggaa tgcaggtgca
    21361 gttccggatc cgaaagggga tcggcagaac gggtgtaatc cagccggtaa acagagctgg
    21421 caaagagcca gaaaggggct acaaagtcct gcaggtaacg catagcccca ctggccagga
    21481 cgttgagcgg aaaatggtct ttcaccttca aaccacggta aaaaccatag atgaccttat
    21541 aacttccgag gtaaaaatgg tagagcaacg attttttatc cccttcgaaa tgagtgaaat
    21601 aaaacagttc gctttcgttg acaaaaaatg cgcgcgatcc tgttacagaa cagtaaagaa
    21661 aagtgttgtt cagataatcg gccttcacct cccagcgtac ttccctttcg gtgatcttct
    21721 ccattttttc gttacggaag atgatttcct ggccggggat gaagttgtag gatttacgca
    21781 gcaggtcatt attctcaatg ttgctgacac attcatcctt acggggataa tcagaggagc
    21841 gaaggacaaa gccctcctcc gttttcagga tgtaatgctg caatggcagg tcgagcgtgg
    21901 cgcttccgat ctgcggcgtc gcctggaact ggaaatggag gtggggcacc ggcgatcgtc
    21961 ctgaattacc caccagtccg ataatatcgc cttttttgac aaaatcacct gccgaacctt
    22021 tcagcgaatc tttcttcaaa tggctcacgg cgctgtagag atgctcagaa tggcggatca
    22081 caacggtatt gccccagttc tggtccaggt tcacctctcc gaccgtattg tcgtcgatcc
    22141 cgtcgagcac ctcttcgatc cagccgtcgg caggcgcgag cacgggcttg ttgtagcagt
    22201 agtagtcgga tacccggtcg ccctcgttcc ggtacgattt ccctttttca tcggtgatct
    22261 cgaaatccca ggcatggcgc cagttatcct tgtgagtgat cgagccgtta tgggcctgcg
    22321 tcacacacca ttcgccccag aacggaagcg ccaccctgaa ccagggcaat ctgccgaacc
    22381 tcagcttgtt gttgcgctgt gaatagaggt tgttctccgg tgaaaatcgc tggtatagaa
    22441 ccagttccgg tttatcgaaa tgccgttccc tgaatttcag aacatagaga aaaacgatga
    22501 cgatgatgtt gaagggaagc gaatagatcg ataactggtt gaaggtaaaa agcatcttga
    22561 atgcggtgag aaggatggcc gtgatcggcg tcagaaggat gatccacagg aaggagtacc
    22621 atgaggggat aataaagaat ccgccgatgg caatggctgt gaggataaaa ttgaagccga
    22681 tgaaggtgta actgagggtt gtcatatcgg ccccgacgac ctggtaaaag atgtaagcgg
    22741 cgtaaaaacc ggccagcgaa aggctgaagg cgatgcggga ataaatgagc agcccgacag
    22801 cgatcagcaa gcccggaaaa agatggtact ggaaaaagat ggccccgagc gaccggagat
    22861 agatgaccag cgactgcggc agctgcaggt tattgaacca ttcccagatt cgcaccatgg
    22921 gcatcccgcc aaggatgtac atttcgttca tgctgtatat cccccggttg ctgatctgca
    22981 gggcaaagaa atcgcgcgaa gccatgaaag tcagccagat ggcgaacagg aacggcagac
    23041 tgagataggg caggttgtac ttgccgatga tcccctccag ggcaacggtg atgaacagcg
    23101 tcagcagggc gctgaacacc agtaccagga aaaactcccc ggtggcggaa aaaaagagcc
    23161 ctaggcccag tccgactaaa agagcattga atccgtaata gcctttgcgg atcttatcga
    23221 cgttcaggcc ggcgagccag gcaacggtgt tggcgatcat aatggagatg agcccggcca
    23281 gccctgaaat gaaatcaaaa aaggtaacgg cgatcagtat ccaggccagg atctggttgt
    23341 tggaaaaaaa taccagggcg taactgttcg taatcccttg gtaataagtg agatatttcg
    23401 acctttcaga gctcaaattt cttcaggtgt tcaggtaccg attcgttccg gttaataatc
    23461 tccagggttt cgcgttcgcg gatgatgtgt gtccgtccat ccgtatcgat cagcaccacc
    23521 ctggggcgca atgtgatgaa ttgcatccac tgggtcatgg tgtaagcgcc cacatggtgt
    23581 atcacgaaat ggtcgccttt gttgagcaac ggcagcacgg cattttcgcg caccacgtcg
    23641 atgttcatgc acaaaggccc gtaaagcatc gtctcttcgg tgtagttgga gaacggctgg
    23701 gcaggagaga tcctgtggtc gtaccagaag gaggtgaaca gcaggttgac ccccacgtcg
    23761 atgatcgtga tccgtttccc ggtggatgac cgtttgttgg agatgacggt tccgaggagg
    23821 tagcccgcct catcgaccag cgcacggccc gtctcaagga tcagcagcgg catcttgccc
    23881 gccgggaagg cggcattgac cagcgatgag gtgatggcct cggccataac gtcgaatccc
    23941 gggttggtgt cggctcccgg aaggaatgag cctttcagcg tgtttcccga aggaaatcct
    24001 ccgcccaggt cgatgtattt gatctcgtgg ttgtagcgct tgtggatgcc gatggccagg
    24061 tcggcgagct tgtaggcagc cgtggcatag gcggaggcgg tcagcatgaa agtgccgatg
    24121 tggcagtgca gcccggccag ttccagcttg cccgagcgca tgatcttgtt cagcgcatcc
    24181 caggcctggc cgttctcgta gttgaacccg aagcggtccc agatcgggaa aacgccggtg
    24241 tccatattga cgcgaatggc tacgcgcggc ttcttccgga gctccgtcga caggttgata
    24301 atcgaataaa gctcttccag gtggtcgata tggatcagcg aatcgttcag gatcgctttc
    24361 cggagatcat cctcgtgttt gtcgggtccg ttgaaaatga tatagcggct gtcgacgccg
    24421 ttggcgatgg ccttgtcgta ttcaaatccc gaaaccacct ccgcccacga tccctcctgg
    24481 tggaaaatgc tgcagacggc attcaggtag ttcgtcttgt acgaccaggc gaactgcacc
    24541 ttcgggtagc gtgtggtgaa tgcctgcttg gcttcgcggt atttttcacg gatcgactgt
    24601 tcggagatga caaacagggg cgaaccgtgt tctttgatca gatctttcac cgccaccccg
    24661 tcgatatgcg tcaccggcac ataagccgtc cgcagaccga atttattggg catgtgggtc
    24721 tccagcctct tcacggccgg cctttcgtat cgctgttttt gtcccatacc atattaaagt
    24781 tcaaagttca aagttcaaag tttaaagtta atgttccata gttccagtgt tccaaagttc
    24841 cagtgttcca ggttccagtg ttccaggttt ccctttacct ttttccctgt tccctgttca
    24901 ctgttcaccg ttcactgttc accgttcact gttcaccgtt cacccttcac cgttcaccct
    24961 tcaccgttca cccttcaccg ttcacccttc accgttcacc
//
LOCUS       DAOPNH010000009        23327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2008_length_23327_cov_7.509539, whole genome shotgun sequence.
ACCESSION   DAOPNH010000009 DAOPNH010000000
VERSION     DAOPNH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_23327_cov_7.509539"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKZ74_01185"
     CDS             complement(<1..249)
                     /locus_tag="PKZ74_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91391.1"
                     /translation="MVWPDPLPDKLFLPDLLNQHLTGGWNGSEWSPCRVWADNVNNVI
                     YPVIGLYDHPSRQKQFLVKLDPAQGGGHMLVMGSAGSGK"
     gene            complement(236..568)
                     /locus_tag="PKZ74_01190"
     CDS             complement(236..568)
                     /locus_tag="PKZ74_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91392.1"
                     /translation="MEIKEVNRNIPENREDSNERFRVRYKDKSKFNLLVVNICRSKTN
                     EIQTFNFKSSDLPDKDSIHFSTSINSGKFQVDWKGISSTPNAKKKIIAKVPDKKKTAT
                     ETTPSGVA"
     gene            complement(684..1535)
                     /locus_tag="PKZ74_01195"
     CDS             complement(684..1535)
                     /locus_tag="PKZ74_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPM91393.1"
                     /translation="MQEADFIAFDTETATAQLSSLCQVGFVVVCNGEVTRKESIFVRP
                     PGNDYAVRNSCLHGIDALKTKDSPEFPEVWEKIGDSFCENLLVAHNASFDLAVLRTTL
                     AYYGLEIPDFKCECTYRMSGLNLAALCDALEIELVNHHDALADATACAIAYLKMKQGI
                     RPDHRLIRPRSGHDFFAGHERITGDLLKPDPEHCDCNHPLFSKKVVFTGVLSSMTRED
                     AARAVKAHGADIDTGITKRTNIVIAGSGAGPAKLKKIEQFNNQGAGIRIIGESEFLGM
                     IMRSYIV"
     gene            complement(2021..2896)
                     /locus_tag="PKZ74_01200"
     CDS             complement(2021..2896)
                     /locus_tag="PKZ74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF022472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HPM91394.1"
                     /translation="MKTKILYPFLLILAIGTSHSYSQTVKIDTLNDYPPVDRTIHEKM
                     LQYGAENVLVVLDIDNTLLTSDTDLGSDIWFQWQTGKLGLKPSPEQKLDRDCLFNEAI
                     CLLYELGTMSLTDSLLPGYIRNWQFKGLTVFALTSRSPQCRPSTERDLKANGIDLSLA
                     PLRTVDGREIHFDKEADSNLSYREGIFMTRGADKGLMLADILARSGRSFKAIIVVDDT
                     GKNIVNIRNNAANYSAADIVLVYYTKILTERVRQNNNDTLTVEQAARMDRDWDELIRL
                     LNAVFPERLEKSECF"
     gene            complement(3080..3502)
                     /locus_tag="PKZ74_01205"
     CDS             complement(3080..3502)
                     /locus_tag="PKZ74_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPM91395.1"
                     /translation="MAEIKTKQTEANVTDFIYAFADTEQKRKDSFELLKLMQDFTGFE
                     PKMWGPSIIGFGNYHYKSDRSRQEGDWPMVGFSPRKAAISLYVYAGTPEQDELLKDLG
                     KFKKGAGCIYVKKLSDIDQDVLKKIMKSTIEFLKEKYG"
     gene            complement(3689..4246)
                     /locus_tag="PKZ74_01210"
     CDS             complement(3689..4246)
                     /locus_tag="PKZ74_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPM91396.1"
                     /translation="MKKTLTQEQVKTLILSLKERFGKYPGRHLGIDWRKVQARLEGNP
                     DRLRSLYEMERTGGEPDVTGQDQNTGEFIFCDCSAESPAGRRSLCYDREALESRKEHK
                     PQINARDMAADMGIEILNEEQYRQLQGLGALDRKTSSWILTPKSIRDLGGALFGDWRY
                     GQVFIYHNGAESYYATRGFRGILRV"
     gene            complement(4312..6510)
                     /gene="katG"
                     /locus_tag="PKZ74_01215"
     CDS             complement(4312..6510)
                     /gene="katG"
                     /locus_tag="PKZ74_01215"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPM91397.1"
                     /translation="METNESKGKCPVTGANSRHSVGSGGTKNRDWWPNQLKLNILRQN
                     SSKSDPMGEDFNYADEFKKLDYEGLKNDLRKLMTDSQEWWPADFGHYGPLFIRMAWHS
                     AGTYRTGDGRGGAGSGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGRKISWADLMIL
                     AGNVALESMGFKTFGFAGGRADVWEPEEDVYWGSETKWLADERYTGERDLDNPLAAVQ
                     MGLIYVNPEGPNGNPDPLLAAKDIRETFARMAMNDEETVALIAGGHTFGKTHGAGDAG
                     LVGPEPEAAGLEEQGLGWKSKFGTGKGGDTITSGIEVTWTTTPTKWSNNFFWNLFGYE
                     WELEKSPAGAHQWRPKHGMGEGTVPDAHDKSRKHAPKMLTTDLSLRFDPAYEKISRRF
                     LENPDEFADAFARAWFKLTHRDMGPKSRYLGPEVPAEDLIWQDPVPAADHPLVSDSDI
                     ADLKAKVLASGLTVTQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAVNNPPQL
                     AKVLETLESIREEFNSAQKGGKKISMADLIVLAGAAGVEKAAKDAGFSVTVPFTPGRT
                     DATQEQTDVESFKVLEPAADGFRNYQKARYSVCSEELLIDKAQLLTLTAPEMTVLVGG
                     MRALNANFDGSAHGVFTMRPGMLTNDFFINLLDMGTVWHPASEANDQFEGRDRATGEV
                     KWTGTRVDLIFGSNSELRALAEVYGSADAGAKFVRDFVKAWDKVMNLDRF"
     gene            complement(6663..7082)
                     /locus_tag="PKZ74_01220"
     CDS             complement(6663..7082)
                     /locus_tag="PKZ74_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPM91398.1"
                     /translation="MKSTEYRNKLVECGLKVTPQRMAILQAIIKLDNHPTADQIIELI
                     RKNNPNISTATVYKVLDTFAACGLIKRVTTATGIMRYDAHLDSHHHLYYADSDKIEDY
                     RDEELSRLLETYFKQKNIPGFEIEDLKLQITGKHTDN"
     gene            complement(7192..8424)
                     /locus_tag="PKZ74_01225"
     CDS             complement(7192..8424)
                     /locus_tag="PKZ74_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91399.1"
                     /translation="MVKYYLLSIFVFLSALSTAQWGPVNNGISNLSFGVKLLGSSDAY
                     IFSGTLADARMYRSGDNGNNWDEIDPPVTGNVPECGFFFEDKYFAGLNSSQECIFYST
                     DDGSSWNSVSGGPQTTWVRGFFSLSGDIFAFTSSAGIYKSTDGGSNWEPANSGLDNYN
                     VIRMESINSKLLAATIGGGVFASTDNGETWFPSNNGIAGSDLNATLVWRMGAGLYYME
                     QGGAAYTSIDEGANWSAWIKPAVMGLAVNEIYRTGNHLYMESRHFAGGLKDSVYYSIN
                     EGFSWDNITDNLSASDLNASGITEFEGYAFIAYNIISPGLGIYRRGISTGTGEMNNPV
                     SIEIYPNPFREKISFASFSRNNIKQINLFDNLGRLVLSESGSETVNTSGLPGGTYIVA
                     VVLADKTTIRRKLIKENR"
     gene            complement(8641..9537)
                     /locus_tag="PKZ74_01230"
     CDS             complement(8641..9537)
                     /locus_tag="PKZ74_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPM91400.1"
                     /translation="MQTKMIGSKIAETRRKLNVSQAQLAERLFISPQAVGKWERGESL
                     PDLIMLNRLAKILGVDLNYFSEDFPSVSNETTFHESPVNQPVEPIFGKQKEKLNWNMS
                     RANWVGADFSGLNNLHEKFSSSNMQHCLFIGSDLSGLLLKGNNVDNCDFSGSGFNNSH
                     IQSSNFARSKFKSCYLKEAEFSASYISGCDFTGSDMTGIVVKSGGFEKNILTDAVLDR
                     SSFIDTQLIDVIFEGALKDCYFENCSFNRVKFQDARIINTFFKNKSLKRIRFINCQVD
                     RMTYAFLKNGKADLTGIMLLEE"
     gene            complement(9679..10458)
                     /locus_tag="PKZ74_01235"
     CDS             complement(9679..10458)
                     /locus_tag="PKZ74_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HPM91401.1"
                     /translation="MTDPSFQIIALTPENIAEYGVCGYKDVKKHLELRRKIDWFHKYY
                     PLGLRIKAVITDTGEYQGMLEYIPGEFAHRPVNAHGYMFIHCIFVGFKSQFKGKGYAS
                     ALLDECISEARLAGMKGVAVVARNGSFMAKKDIFLKKGFTQVDRAQPDFELMAMKFDE
                     RAENPAFKELNPANYPEGITILRSPQCPYSVKNVEAIMLTAQRMNLDPNLIEMDDHVT
                     AQKSPCAFGTFCIIHKGRILSHHPISNTRFENIVKSVKREA"
     gene            complement(10552..10872)
                     /locus_tag="PKZ74_01240"
     CDS             complement(10552..10872)
                     /locus_tag="PKZ74_01240"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM91402.1"
                     /translation="MKPINLNNVNSREIIPGYRARFVNSDNMTLAFWDISAGSPMPEH
                     SHPNEQVTTLLEGEFMMTIGGDTFHLKPGSVLVIPPDVSHKGHPLTDCRILDVFYPRR
                     EEYK"
     gene            complement(10899..11348)
                     /locus_tag="PKZ74_01245"
     CDS             complement(10899..11348)
                     /locus_tag="PKZ74_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91403.1"
                     /translation="MNKRFLIAGLITSLINLLLHGLTYALILKDFYRSYPAGSEEFIK
                     QLHRGPDQLIGWAMAATALAMGYFITLIMKWSGARTFLSGLKYASIAGILFWASVNFG
                     LYASSNMFSLPAVFVDLACSAAVMTLAGASAAWMLGRNPKGWRPFFS"
     gene            11591..12241
                     /gene="elbB"
                     /locus_tag="PKZ74_01250"
     CDS             11591..12241
                     /gene="elbB"
                     /locus_tag="PKZ74_01250"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPM91404.1"
                     /translation="MKKFAVVLSGCGVFDGAEIHEATLTLLAIAQAGATYEIFAPDID
                     QHHVINHYTHKEMAEKRNVLVESARIARGRISPLSKFDARHFDAIIFPGGFGAAKNLS
                     TVAFDGPEAKVNPDVEAAVKAMHRAGKPIGALCIAPAVIARILGGVEVTIGNDPGTIG
                     AIRKMGGKHVETTHGEVVADKKNKIFSTPCYMLDATIVDIYHGATRIVHEMLNFKC"
     gene            12327..12476
                     /locus_tag="PKZ74_01255"
     CDS             12327..12476
                     /locus_tag="PKZ74_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91405.1"
                     /translation="MKPNAEIRNQIMQVVENQMKMDDPPETNQTYKRLIRLGFNEFDS
                     KAMIG"
     gene            12639..13694
                     /locus_tag="PKZ74_01260"
     CDS             12639..13694
                     /locus_tag="PKZ74_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPM91406.1"
                     /translation="MKIPHSTFNIQHSTLMNRITSLFGIKYPIIQAGMIWCSGWELAS
                     AVSNAGGLGLIGSGSMYPDVLREHIRKCKAATDKPFGVNLPLIYKQVEDHIQVILDEG
                     VKIVFTSAGNPAKYTKFFKDHGLIVVHVVANTKFAKKCEEAGVDAIVAEGFEAGGHNG
                     LEETTTMTLIPMVRQVTSLPLIAAGGIATGQQMLAAFALGAEGVQIGSRFVASQESSA
                     HPAFKQKVLEANDGDTALVLKKVIPVRLIKTPFYERVKEAENRSASREELMELLGHGR
                     AKLGMFEGDIDEGELEIGQVAGLIREVVPAAEIVKEIVRDFNLTIGRFDDWAKGLSIV
                     DIRLSIVYCLFYFGFRF"
     gene            13829..16912
                     /locus_tag="PKZ74_01265"
     CDS             13829..16912
                     /locus_tag="PKZ74_01265"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM91407.1"
                     /translation="MPLRTIAGILILINLACFGQLDNYYLKSYSVSDGLPNNHVRWIT
                     QDRDGFLWLATWDGLARFDGVRFKIYRHDPNDSTSVSFFEIIKVAVDSGNRVWVFAGG
                     RLCRYDRSRDNFIRYNLSHFPTLGPGDGPGEFFDILIDPEGSLLVACGFGFYRYDPES
                     DRFASIHPAGNKAGNFIPSFPGFDNMNNLWLMWVDKTHADYAEVYKCSYKHGQVLSVV
                     DSFVCDPVNFRSRFTNETIRFNYYLSSAGEAWMASSLGLYYLQNDTFKLSQNKIPKGH
                     FSNTGMILWSGHNNGLMIDYLPENHVDTLFEDGRIETVIAYHHDELGNVWFSDMSEPS
                     VKNGLSHCFRTGNYFRHYLTGLEGDEPYVIYGLYKDRVGNIWAGGRPNTHIVKISPDG
                     VEQQINLPFHPVKYFNVPRNIEPDLNGHLWIAFFSDYLFSLDPGTLQYTDFSGINMTN
                     PTNLSKINYRLVKPYGRDLLFSAGAGKLYKVNTLTGNVITSSAPNGWDIFCLYPVGDN
                     TLWVGLSGRLLLTDFGFNKQEIIPVIDPFYNIEDICPGDSSYLWLALLGGGICRFDTL
                     NHQTSFFTTFNGLAHNTVYSIRKDQAGNLWISHNLGISMFNPVTKSFTNYDEKDGLKI
                     KEFNSEAACQTDQGEILFGGIGGIVSFFPDSIRNQPDDDHSKLMISDFRIANKVYPFD
                     KPVSEMKKVRLPKGTDNFQLEFIKPDFRNGDEIRYRYTLGGRMDKWTETDCDHRRVNF
                     TSLRPGDYEFIVECTDSNGEWANSTMLLITIPPYFYQTIVFRLLLAIVVMVVLGLFFY
                     MKLKQIRLSEHKKQEQLRLETLRGQMNPHFIYNSLNSINYFISLNDRLNANQYITDFS
                     RLMRSIMTNSAQDYITLESEIQAIKDYLALEHLRFSNKFDYEIKTDEALDLVITEVAP
                     SLVQPFIENAIWHGLRYIENRKGFLSVRFIMEGNGNLVCYVDDDGIGRKLSVKLKTGD
                     QKKRNSRGIAIVTERLAIINSMQKSRYSVRVMNMYDERDETGTKVRIEIPFRRSL"
     gene            16909..17655
                     /locus_tag="PKZ74_01270"
     CDS             16909..17655
                     /locus_tag="PKZ74_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91408.1"
                     /translation="MIKCIIVDDEFPARECIKNYLKDYCPDVEVVAHADSANSAYKEI
                     LDHHPDLLFLDVEMPNGSGFDLLRMMNKIDFHIIFVTAHEEYAVMAFRFSAMDYLLKP
                     INIKELVTAIDKVRTEISRKSEAVNLETLKRITQMGPAEWDQIVIKHSEGFKVVYLRD
                     IISCEADSYCTIFHISGNKKLVSSKNLKYYEDILTPHGFQRIHNSFLINLSHVVEYSS
                     EGVVALSEGQLAHLGNTYKKKFMDYFERNK"
     gene            18166..18993
                     /locus_tag="PKZ74_01275"
     CDS             18166..18993
                     /locus_tag="PKZ74_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91409.1"
                     /translation="MMWNINRSALIILAALLIEAGALTAQNNNQNDKNFFIRGYAGAQ
                     GKISLIFPVELTEYTQDFYDHLLDEWGNYGYFPDMMNIPPVYSGYGYTLKAGIRLFNV
                     FQVEPWWEMYKSFPLQIRLDAYYYDGYSGYSREIDMNYKFRPMYDALGASLMFVPGSS
                     DKPVFFTFGGGIGSYIGKLSIETDGSETINDNTTPVYDYEEYSGKAIGYHASIGMTYV
                     PWKYLELETLLTGRYVKIPEMENAAGYVLTNSYDDNQPVVMQLSGIDFRFGIKFIFP"
     gene            complement(19032..19496)
                     /locus_tag="PKZ74_01280"
     CDS             complement(19032..19496)
                     /locus_tag="PKZ74_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91410.1"
                     /translation="MIYKSRPQKFNSLILSILCINRSDGCWCYGYGPYQQPPDLRYKY
                     PVEVNDTWVTSWVAPPYPTSVTCLSTNTSIGGYTDCIVYNFYLPMKYKSSLAFDYLRM
                     NEEDFPDEIEKKYSSFDIYDYYIPGIGMVGWETYLDGALFVKAVLVDYDVAR"
     gene            complement(19486..20133)
                     /locus_tag="PKZ74_01285"
     CDS             complement(19486..20133)
                     /locus_tag="PKZ74_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91411.1"
                     /translation="MKSELFLFRKFYFGMRVFIGIIFSLILSINAFSQSALKVHLPMG
                     KFYGDAIDSTGFTPIFNVTSFTYQAYATLSENEKVVLFVDAGLGVIGTKQIFENDKYK
                     ARVYGLQLGMYFKYNILKPTLTPYIYTGINLDILFANELYLATISGNNTYRTEVGHRV
                     LFPEYVVGVGFTFLQGGLSLDLQDKIGLISVVNGESIKSNLISIGIIVHATIFDL"
     gene            complement(20160..22268)
                     /locus_tag="PKZ74_01290"
     CDS             complement(20160..22268)
                     /locus_tag="PKZ74_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM91412.1"
                     /translation="MKTLILFFLLIFTTTWVLAQEVLYEDSFDTKEENWVSTDDYVEE
                     FKNGELILHGYLRKETVLVMREIRIDPGKDFSIKCSIKYADGHTKKPFGLMALDTRYT
                     DKMGIYKFKIYPKDGYAIDHQVQYRYKPVEVLPKTTKTGIVKGPGEYNQLELRNTNGV
                     VEFFINDHKVWSSSDFDFCISIVGFMNEGYQNVKVDYFVAKQDGWQNINLVEESGKIF
                     LKENLGENVNSKAEELAPIISADGQTLYLTVESDKNNIGKEDTQDIWYSTLNPDSTWS
                     RRVNIKKPLNNESNNSVVYCSPDNNSLVVINRYDKSGKSIGAGLSKSERTKGGWTIPT
                     ALNIKNYYNDNLYCSSNLSPSQNEMILSLERKDSYGEKDFYISFRQDDDSWSEPLNMG
                     SDINTFGDEGTPFIAADNVTLYFSSNAYPGYGSHDIYITRRLDDTWTKWSKPQNLGPS
                     INTPGWDAYFTIPASGDYSYLVSTDNSFGNEDIFRIKVSEAAKPNPVALITGKVYNLK
                     SSGFIEASITYFDLETNEEIGIARSNPDDGTYKISLPSGHAYSFYARKEGYFSVSENI
                     DLTTLKEYSEITRDLYLAPIDTGTTIRLNNIFFDYNKTELLEASFFELDRLVNIMNEN
                     PQIVIQIAGHTDNMGTDAYNQKLSEDRAKAVYEYLKNKSIALSRISFVGYGESRPVET
                     NDTEEGRAFNRRVEFVIVSN"
     gene            complement(22301..>23327)
                     /locus_tag="PKZ74_01295"
     CDS             complement(22301..>23327)
                     /locus_tag="PKZ74_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91413.1"
                     /translation="NDINIIQLDRNMGIVKSCSLSFDKQQYLAFCEGIDVNTDEEGFE
                     EYYLIFGPAKEDLSKTAFDFKFVQMNNKLEVTGEIPILSHYSFWKIDDMIAPESKEEA
                     YFFGPADNREVTYYFAAAGGEFGMGNPFVGVDFTATQLMKVKNGQVEYIEATLTDDYT
                     KKLQVPPGQEKLPAYRGNKFEKDAYYVADDGKFFVAGQNWSTTFNPVAGTSKDVYTDV
                     LAFYFNPQGQLVSQYGLDTRENNSCAKEYGTGQFFIEGLGHQYLYWVILETSSMWEGD
                     LLQYPTIGKIDMTTGEISAFRIYGSESFFVDDQFPELYSPDPGKVIYLCADKFKRNVG
                     FLRLIFD"
BASE COUNT         6039 a         5476 c         5354 g         6458 t
ORIGIN      
        1 tttgcccgaa ccggctgaac ccatcaccag catatgaccg ccaccctggg cggggtccag
       61 ttttaccaag aactgcttct ggcgggatgg gtgatcatac agcccgatta ccgggtagat
      121 aacattgttg acattatcag cccagacacg gcatggactc cactcacttc cattccagcc
      181 accggtgaga tgttggttca aaagatctgg gaggaacagc ttatcgggca gcgggtcagg
      241 ccacaccact gggggttgtt tcagttgcag tttttttctt atcaggcact ttggctatta
      301 ttttcttttt agcattagga gtagaagata ttcccttcca atcaacctga aattttccac
      361 tgttaatcga agttgaaaag tgaattgaat ccttgtctgg taaatctgaa gatttgaaat
      421 tgaaagtctg gatttcattg gtctttgaac gacagatgtt aactactaaa aggttaaatt
      481 tgcttttgtc tttgtagcga actctaaatc tttcattcga atcttctctg ttttccggaa
      541 tattcctatt aacttccttt atctccattt tctaaatatt tatagtttca gttgtcttta
      601 tttcagcctt gcccataact ccgataaaac atcatttctg ttacacagca aacgataatt
      661 atttcatttc aaatcacttt ctgttaaaca atataacttc tcattatcat ccccaaaaac
      721 tcactctccc cgataatcct gatcccggct ccctgattat taaactgctc aatcttcttc
      781 agttttgccg gtcccgcgcc acttccggcg atcacaatat tcgtccgttt ggttatgccg
      841 gtatcgatat cggcgccatg agcttttaca gcccgggcgg catcctcgcg ggtcatgctg
      901 ctcaatacgc cggtgaaaac cactttcttg ctgaataagg gatggttgca gtcgcaatgc
      961 tccgggtcgg gtttcagcaa atcgccggtg atacgctcgt ggccggcaaa gaagtcgtgg
     1021 ccggaacggg gcctgatcaa acggtggtca ggacgaatac cttgcttcat cttcaaataa
     1081 gcgatcgcac aggcggttgc atcggccagg gcatcgtgat ggttcaccag ttcgatctcc
     1141 aaagcatcgc ataaagcagc caggttcagc ccgctcatcc gataagtgca ttcgcacttg
     1201 aaatctggta tttcaaggcc ataataagca agtgtagtcc tcaaaacagc caggtcgaat
     1261 gaagcattat gcgccaccag caaattttcg caaaacgaat ccccgatctt ctcccatacc
     1321 tcgggaaact caggactatc cttggttttc agcgcatcaa tcccgtgcag acaggagttc
     1381 ctcacggcgt aatcatttcc cggcggccgc acaaaaatcg actctttccg ggtaacttcc
     1441 ccgttgcaaa cgacaacaaa acctacctgg caaaggctgg aaagctgtgc cgtcgccgtc
     1501 tccgtatcaa aagctatgaa atctgcctct tgcatctatt ttctctaaac aattgctttt
     1561 cccaaatacc cgatggtacc tgggaaaaag taaacagtaa aacgattctt cacctccgcg
     1621 ggcctgcggc atcagcccgg tttcaacttt gcagagtcaa atatagggtg ttatttgata
     1681 tgttttacat gcagggataa tttggaggga gtctaaagag ggttccaggt tccaggttcc
     1741 aaaatccaaa ttccaagcac caagcaccaa acaaattcca aaatttaaaa tcacaacctt
     1801 atgactacct tctaacaacc ttataaccac cttatgacta ccttctaacc accttctgac
     1861 tactttatga ccactttctg accaccttat gacaagtgac aaatttcccg caggtgaacg
     1921 cagatttaaa gcgcagattt gcgctgatca tcatttataa caggaaatcc ctctgcgaag
     1981 atctgcttct cttctgcgaa aatcagtgag aaaaaaactg ctaaaaacac tccgatttct
     2041 ctaacctctc cggaaagacc gcattgagca aacggatcag ctcatcccag tcgcggtcca
     2101 tccgggcagc ctgctccacg gtcagggtgt cgttgttgtt ctgcctgacc ctttcggtga
     2161 ggatcttagt gtagtaaacc aggacaatgt cggcggcgct gtagttagcg gcattattcc
     2221 ggatattgac aatgttcttc cctgtatcgt cgacgacgat aatggctttg aagctccggc
     2281 ccgaccttgc caggatatcg gccagcatca ggcctttatc ggctccccgg gtcatgaaaa
     2341 tgccttcccg gtagctcagg ttgctgtcgg cctctttatc gaaatgtatt tcccggccat
     2401 caacggtccg gagcggggcc agggagagat cgatgccatt cgctttcagg tcgcgttcgg
     2461 tcgagggcct gcactgaggg ctgcgcgagg tcagcgcgaa aaccgtcagc cctttgaact
     2521 gccagttcct gatatagccc ggcaacaggc tatcggtcag cgacatggta ccgagctcat
     2581 acagcaggca gatggcttcg ttgaaaaggc aatcccggtc gagcttctgc tccggcgaag
     2641 gtttaagacc cagtttaccc gtctgccact ggaaccagat atcgctgccc aggtcggtat
     2701 cgctggtcag gagggtattg tcgatatcca ggaccaccag tacgttttcg gcgccgtatt
     2761 gcagcatctt ttcatggatt gtcctgtcaa ccggcgggta atcatttaac gtgtcgatct
     2821 tcacggtttg actgtaggaa tggctggttc cgatagccag gatgagaaga aacggataca
     2881 ggatcttggt tttcatcttg gttgggttgt tgttaatgca tcaaattgtt taaacacaaa
     2941 gaacacaggg agtttcacaa tgtacacaag ggatttcaca aaagcgcaca aagaatttca
     3001 caaagttgca caaagtaatt gattaaccca aacctttgtg tacccttgtg aaactccctg
     3061 tgttccttgt gtttaaattc tacccgtact tctctttcaa aaactctatc gtcgacttca
     3121 tgatcttttt caacacatcc tgatcgatgt cagaaagttt tttaacataa atacatccgg
     3181 cgcccttttt gaattttccc aggtctttca acaactcatc ctgctccggg gtgccggcgt
     3241 aaacgtagag ggaaatagcc gctttccttg gcgaaaatcc caccatcggc cagtcgcctt
     3301 cctgtcggct cctgtcggat ttgtagtgat agtttccgaa cccaattata gagggacccc
     3361 acatttttgg ctcgaagccg gtaaaatcct gcattagttt cagaagttcg aagctgtctt
     3421 ttcgcttttg ttcagtgtca gcaaacgcgt aaatgaagtc tgtaacgttt gcttctgttt
     3481 gctttgtttt aatttctgcc atcgccaaat tcttgagtaa ttattttgaa ttttacggct
     3541 acgactttcg ggaagtcata tagccatttt agtgtaaatt gagatggcat ctcagaatgg
     3601 tcgtttcgat gtgtgcaaaa ccttgtcaaa caagcaaaag ctaaggtttt tcatggcgga
     3661 atgagatgcc atctcaaaat cactcagatc aaacccttaa tattccccga aatcccctgg
     3721 tggcgtaata cgactctgcg ccgttgtgat agatgaatac ttgtccgtaa cgccaatccc
     3781 caaacagggc acccccaagg tccctgatgc ttttaggggt cagaatccag ctggaggttt
     3841 tacggtctaa agccccaaga ccctgcaatt gtcggtactg ctcttcgttc agaatttcta
     3901 ttcccatatc cgctgccata tcgcgggcgt tgatttgcgg tttgtgttct ttcctggatt
     3961 ccaatgcttc ccggtcgtag caaaggcttc tgcgacccgc agggctttcc gccgaacaat
     4021 cgcaaaaaat aaattcacct gtattttgat cctgccccgt cacatcgggt tcccctccgg
     4081 tccgttccat ttcgtagagt gaacggagtc gatccggatt cccttcgagc ctggcctgca
     4141 cctttcgcca atcaatcccc aaatggcggc cagggtattt cccaaaacgc tcttttaaac
     4201 taagaatcaa tgttttaacc tgttcctgcg tcagagtttt tttcattttc atataaataa
     4261 atgagactcc atctgccttt aatgagatgg agtctcaatt tgtaatagca attagaaccg
     4321 gtcgaggttc atcactttgt cccaggcttt cacgaaatca cggacgaatt tcgcaccggc
     4381 gtcggcactt ccgtaaacct cggccagggc gcggagttcg gagttcgagc cgaagatgag
     4441 atcgacgcgg gtgccggtcc acttcacctc gccggtagcc ctgtcacgcc cttcgaattg
     4501 atcatttgcc tcagacgcag gatgccatac ggtgcccata tccagcaggt ttatgaagaa
     4561 atcgttggtc agcatgcccg gacgcatggt gaagacgccg tgcgccgagc cgtcgaaatt
     4621 ggcgttcagc gcgcgcatac cgcccaccag caccgtcatt tcgggagcgg taagggtcag
     4681 caattgcgct ttgtcgatca gcagttcttc ggaacagacg gaataccggg ctttctggta
     4741 attgcggaag ccgtcggcgg ccggttcgag cactttgaac gattccacat cggtctgttc
     4801 ctgcgtggcg tcggtccttc cgggggtaaa cgggaccgtt acgctgaagc cggcgtcttt
     4861 tgcggctttc tcaacacctg cggcgccggc caggacgatc aggtcggcca tggagatctt
     4921 tttgccgcct ttctgtgcgc tgttgaattc ttcccggatg ctttcgagtg tttcgagtac
     4981 tttcgccagt tgcggagggt tgttcaccgc ccagtctttc tggggcgcca ggcggatacg
     5041 ggcgccgttg gcgccgccgc gcttgtcgga gccccggaag gtcgaggccg atgcccaggc
     5101 ggtagaaacg agctgtgtga ccgtcagccc ggaggccagc acttttgcct tcaggtctgc
     5161 gatatcggaa tcgctgacca gcgggtggtc tgctgccggc accgggtcct gccagatgag
     5221 gtcttcggcg ggcacttcgg gtccgagata gcgcgatttc ggccccatat cgcggtgggt
     5281 cagcttgaac caggcgcggg caaaggcatc ggcaaattcg tcggggtttt ccaggaaacg
     5341 gcgggagatc ttctcgtaag ccgggtcaaa ccgcagggag aggtcggtgg tcagcatttt
     5401 gggcgcatgc tttctggatt tgtcgtgggc atccggcacg gtgccttcgc ccataccgtg
     5461 cttcggccgc cactggtgtg cgcctgccgg gctcttctcc agttcccatt cgtagccgaa
     5521 caggttccag aagaaattat tgctccattt ggtgggggtg gtagtccagg tgacttcgat
     5581 accgctggtg atcgtatcgc cgcctttacc ggtgccgaat ttgctcttcc agccgaggcc
     5641 ctgctcttcc aggcctgccg cttcgggttc ggggccaacg agtcctgcat cgccggctcc
     5701 gtgggtcttg ccaaaggtat ggccgccggc gataagcgct acggtctctt cgtcgttcat
     5761 agccatccgg gcgaaggtct cgcggatgtc tttggctgcc agcagcgggt cgggattgcc
     5821 gttgggccct tccgggttca cgtagatcag tcccatctgg acggccgcca gggggttgtc
     5881 gaggtcgcgt tcgccggtat agcgttcatc ggccagccat ttggtctccg atccccagta
     5941 aacgtcttct tccggttccc agacatcggc acggccgccg gcgaaaccga aggttttaaa
     6001 gcccatcgat tccagcgcta catttccggc caggatcatc aggtcggccc aggagatctt
     6061 ccggccatat ttctgcttga tcggccagag cagccggcgg gctttgtcga ggttcacgtt
     6121 gtcgggccag ctgttaaggg gggcaaaacg ctgctgtccg ctcccggcgc cgccgcggcc
     6181 gtcgccggtg cggtatgtgc cggcgctgtg ccaggccatc cggatgaaca acgggccgta
     6241 atggccgaaa tcggccggcc accactcctg cgagtcggtc atcagcttcc ggaggtcgtt
     6301 tttcaatcct tcataatcga gctttttaaa ctcatcggca taattaaaat cttcacccat
     6361 cgggtcggat ttcgatgaat tctgccttaa aatattcagc tttaactggt ttggccacca
     6421 gtcgcggttc tttgtccctc ccgaacccac actgtgcctg gagttagccc cggttacagg
     6481 gcattttcct ttgctctcat ttgtttccat tatttataag tttaattaaa gttatttgta
     6541 aatatttcat tattcatttt ttgccaccaa aacactaaag atatttgatt gatgtaaagc
     6601 attttgtgag ctttcctgtt ttaatgtttt tgtggcttat aattcttctt gttttttaaa
     6661 aatcaattat ctgtatgttt tccggtgatc tgaagcttca gatcttcaat ttcaaaaccc
     6721 gggatgtttt tctgcttaaa ataagtttcg agcaaccggc tgagttcctc atccctgtaa
     6781 tcctcgatct tgtcggaatc ggcatagtag aggtggtgat ggctgtcgag atgggcatca
     6841 tatctcataa tgccggttgc cgtggtgacc cttttgatga ggccgcatgc ggcgaaggta
     6901 tccagcactt tgtagaccgt ggcggtagag atattcggat tgtttttacg gatcagttcg
     6961 atgatctgat cggcggtggg atggttatcc agtttgatga tcgcctggag gatggccatc
     7021 cgttgaggcg ttactttcaa accgcactcc accagtttat tcctgtattc cgttgacttc
     7081 ataatgaatt attatcatat aagaataatt ctcttttaag aattaacaaa gataataata
     7141 aaaagttcag aagtcaagtc tttcgggacg ccatcctttg ggacgccatc cctacctgtt
     7201 ttctttaatg agtttccgcc ggatggtcgt cttatcggcc agaaccactg ctacgatata
     7261 ggttccgcca ggtaaccccg aggtattcac tgtttcgctg ccactttccg acagcaccag
     7321 cctccccagg ttgtcgaaca gattgatctg tttgatgttg ttacgggaga aactggcaaa
     7381 tgatatcttt tcacggaagg gattggggta gatttcaatg ctgaccggat tattcatttc
     7441 ccctgtaccg gtagagatac ctctgcgata tatccccagg cccggtgaaa tgatattata
     7501 ggcgataaat gcatatccct caaactccgt tataccggaa gcgttcaggt ctgaagcgga
     7561 taaattatcc gttatattgt cccaggagaa tccttcattt attgaataat acacactgtc
     7621 tttcaatcct ccggcaaagt gacgcgactc catgtaaaga tgattgcctg tgcgatatat
     7681 ttcgttgact gccaggccca taaccgcagg tttgatccag gccgaccagt tagctccttc
     7741 atcaatactg gtataagcag cgccgccttg ctccatatag tacaaaccgg cacccatcct
     7801 ccagaccagt gtggcattca gatcggaacc ggcaataccg ttatttgagg gaaaccaggt
     7861 ttcgccgtta tctgtcgaag caaaaacgcc tccgccgatg gttgcggcca ggagttttga
     7921 attgatcgat tccatccgga tcacattgta attatccaat ccggaattag cgggttccca
     7981 gtttgagccg ccatcggtcg atttataaat tcccgcactg gatgtaaaag caaagatatc
     8041 tcccgataaa ctgaaaaaac ctcttaccca ggtggtctgc ggccctcccg aaacgctgtt
     8101 ccaggaggaa ccgtcgtcgg tggaatagaa aatgcactcc tgggatgagt ttaatcctgc
     8161 aaagtatttg tcttcaaaaa agaagccgca ttccggcaca ttgcccgtta ccggtggatc
     8221 gatttcatcc cagttattcc cattgtcgcc tgaacgatac attctggcgt cggccagggt
     8281 tccggagaaa atataggcat cggatgaacc aagtaatttt actccgaagg acaaatttga
     8341 aatgccgttg ttgaccggcc cccactgggc ggtgcttaaa gcgctgagaa agacaaagat
     8401 tgataagagg tagtacttta ccatattcgg ctaaatatta atgattagtt gtttaactgg
     8461 tcatgaaaac aactcgtcag gcaaaaatgt tcaaccggat ttctttttca gatttcgaaa
     8521 catgccgctt aaattgtcga gaaaagacag gaaaaaaaag agttaatacg gtttatttct
     8581 taaagtatgt ttaacaaaca ggatattgaa gttgttcagt aacaaaaacc ggattccctt
     8641 tcattcttcc agcagcatga taccggtcag gtcggctttc ccgtttttca ggaaagcata
     8701 agtcatccgg tccacctggc agttgataaa gcggatccgc ttcaggctct tgttcttaaa
     8761 gaaagtattt atgatccttg catcctggaa tttcaccctg ttaaaggaac agttctcaaa
     8821 atagcaatct ttcagggcgc cttcgaaaat gacatcaatg agctgagtat caataaaaga
     8881 ggaacggtct aacacagcgt cagtcaaaat atttttttcg aaaccacctg atttaacaac
     8941 tatcccggtc atatctgaac cggtgaagtc gcaaccgctg atgtagctgg ctgaaaattc
     9001 agcctctttc aggtaacagg atttgaattt acttcttgca aagttggaac tctgaatatg
     9061 gctattgttg aaacctgatc ctgagaaatc acaattatcg acattattgc cttttaaaag
     9121 aagtcccgac aaatcggaac ctataaacag gcagtgctgc atattggatg aactgaattt
     9181 ttcgtggagg ttattcaatc ctgaaaaatc ggctcccacc caattcgcgc gcgacatatt
     9241 ccagttcagc ttttccttct gcttaccgaa aatgggttca acaggctgat taacaggtga
     9301 ttcatgaaat gttgtttcat tactgacaga gggaaagtct tcagagaaat agttcaggtc
     9361 aactccaagg atttttgcga gccggttaag cataataaga tccggaaggg attccccgcg
     9421 ttcccattta ccaaccgctt gcgggctgat gaacaagcgt tcggcaagtt gtgcctgcga
     9481 cacatttaat ttcctgcgtg tttcagcaat tttactccca atcattttag tttgcatcgt
     9541 ctatttttat tatttgaatt ataatttttt cgatacggca aagatatcat gcctcatatc
     9601 gaaaatcaaa aaaaactaaa cacgattggt tgtaattatg atactattag ttgtttagca
     9661 caaccattgg ttgtgttttt acgcttcacg cttcacgctt ttcacaatat tctcaaaacg
     9721 ggtattgctg atcggatgat ggctcaggat cctgcctttg tggatgatgc agaaagttcc
     9781 gaaagcgcat ggggatttct gtgcggtaac atggtcatcc atttcaatca ggttgggatc
     9841 cagattcatc ctctgagcgg tcagcatgat ggcttccaca ttcttcacag aataagggca
     9901 ttgcggtgaa cggaggatgg taatgccttc cggataattg gcgggattaa gttccttaaa
     9961 agccggattt tctgcccttt catcaaattt catcgccatc agttcgaaat ccggttgtgc
    10021 ccgatcaacc tgggtaaatc cttttttcag gaagatgtct ttctttgcca tgaaggagcc
    10081 attgcgggca actacagcaa cacctttcat accagcgagc cgggcttcag agatacattc
    10141 gtcaagaagt gcagaagcat agccttttcc tttgaattgg cttttaaagc ccacgaagat
    10201 gcaatggatg aacatatagc catgggcatt gaccggccgg tgggcgaatt caccgggtat
    10261 atattccagc attccctggt attcgccggt gtctgtaatt acggctttaa ttcttaatcc
    10321 cagagggtaa tatttgtgga accagtcgat cttacgtcgc agttccaggt gttttttcac
    10381 atctttatag ccacagacgc cgtattcggc gatgttttcg ggtgtaaggg cgatgatttg
    10441 gaaggaaggg tcggtcataa tcctggtctg ggtattaaaa ttcgggtaaa gatataaaat
    10501 tgagactcca tctcaaactg ccgagatgga gtctcaggga aagaggctgc atcacttata
    10561 ttcttccctt cgcggataaa agacatcgag gatcctgcaa tcggtcaatg gatggccttt
    10621 atgactgaca tcgggcggaa tgaccagcac cgatccgggt ttcaggtgaa aggtatcacc
    10681 accgattgtc atcatgaatt ctccttccag gagagttgtc acctgctcgt taggatggga
    10741 atgctcaggc atgggcgaac cggcagaaat atcccagaaa gccagagtca tattatcgct
    10801 attcacaaac cgggcccggt agccggggat gatttcgcgg gaattgacat tgttcaggtt
    10861 gatgggtttc atattgaatt gttttagaga atcgaaagtt agctaaaaaa gggacgccat
    10921 cccttgggat ttcgtcccag catccatgct gcagatgccc cggccagggt catcaccgcg
    10981 gcgctgcagg ccaggtctac aaacacggcg ggcagggaga acatgttgga ggaggcgtaa
    11041 agcccgaaat tcaccgaagc ccagaacagg atcccggcaa tggacgcata tttcagtccg
    11101 ctgagaaagg tccttgcgcc ggaccatttc attataagag tgatgaaata gcccatcgca
    11161 agggcagtgg ccgccattgc ccatccgata agctgatcag ggcccctgtg aagttgtttg
    11221 ataaattcct cgcttccggc cggatatgac cggtaaaaat cttttaaaat taacgcatag
    11281 gttagtccat gcagtaatag gttgatcaat gaagtgatta acccggcgat taagaatctt
    11341 ttgttcatcc tcataaatta tttacaacca aactattaaa tattaatctc ccaaaccagt
    11401 gttgtttgac attttgccaa aatgcgtttg caatcggtca ggaaatatta atttcccgga
    11461 cgacgatttt attgagactc catctcaatc tgcccagatg gagtctccat tcatcgagac
    11521 tccatctcga aaaaatgaga tggcatctca tccattttcc ctatttttac actactaaac
    11581 ttgtattcct atgaagaaat ttgccgtagt attatccggg tgcggcgtgt tcgacggcgc
    11641 cgagattcat gaagccacac tgaccctgct ggctatcgcc caggccggcg ccacgtatga
    11701 aattttcgcg ccggatattg accagcatca tgtaataaac cattatactc ataaggagat
    11761 ggcggagaag cgcaatgtgc tggtcgaatc ggcccgcatt gcccggggca ggatcagtcc
    11821 cctcagtaag tttgatgcac gacatttcga tgccatcatc tttccgggcg gctttggagc
    11881 tgccaagaac ctgtcgactg tggctttcga tggcccggaa gcaaaagtga atcccgatgt
    11941 ggaagcggcc gtcaaagcca tgcaccgcgc cggaaaaccg atcggggcgc tgtgcattgc
    12001 cccggctgtc atagccagga tcctgggcgg cgttgaagtg accatcggca acgatcccgg
    12061 caccatcggc gccatccgga aaatgggcgg aaaacatgtg gaaaccaccc atggcgaagt
    12121 ggtagcggat aaaaagaaca agatcttcag cacaccgtgc tatatgctgg atgcaacgat
    12181 cgtggatatc tatcacgggg caaccagaat cgtgcatgaa atgttgaatt ttaaatgttg
    12241 aattttgaat gcgggaagtt cttgtaagtg ttaagttttg agtgttaagt tttgagtgtt
    12301 aagttttaag tgagggaatt tattttatga aaccaaatgc agaaattaga aaccagatca
    12361 tgcaggttgt tgaaaaccag atgaagatgg atgatcctcc tgaaaccaat cagacttaca
    12421 aaagattaat aagactcggt ttcaatgaat ttgacagtaa agccatgatc ggttagtgcg
    12481 ttgctgttga aatcttcaat atcatgaaat tcaggaatcc ttttgatgaa caacgtttca
    12541 taaaaaacct taataaattg ccggaagagc catttgaatg actttgaacc ggccaatgaa
    12601 atatataaac attatttcct ttcccgatct aaacattaat gaaaatcccg cattcaacct
    12661 tcaacattca acattcaaca ttaatgaaca gaatcacttc tcttttcggg ataaagtacc
    12721 ccatcatcca ggccggcatg atctggtgct ccggctggga gctggcctcg gcggtcagca
    12781 atgccggcgg gctgggcctg atcggctccg gatcgatgta tcccgatgtg ctcagggaac
    12841 atatacgcaa gtgcaaagcg gccaccgata agcctttcgg cgtaaacctc ccgctgatct
    12901 acaaacaggt tgaagatcat atccaggtta tactcgacga gggagtgaag atcgtgttca
    12961 cctcggcagg caacccggca aagtacacaa agtttttcaa ggatcacgga ctgatcgtag
    13021 ttcatgtggt ggccaacacg aaattcgcga agaaatgcga agaagcgggt gtcgatgcga
    13081 tcgtcgctga aggttttgaa gcgggaggcc acaacggcct tgaagagacc accactatga
    13141 ccctcattcc gatggttcga caggtgactt ctttgcccct gatcgctgcc ggcggcatcg
    13201 caaccggcca acagatgctg gcagcatttg ccctgggcgc tgaaggcgtg cagatcggca
    13261 gccggttcgt ggcgtcgcag gaatcgtcgg cccacccggc ctttaagcag aaagtgctgg
    13321 aagccaatga cggcgatacg gcactggtcc tgaaaaaagt catcccggtg cggctgatca
    13381 aaactccgtt ctatgaacgc gtaaaggaag cagaaaaccg cagcgccagc cgcgaagagc
    13441 tgatggaact cctgggccac ggacgggcga aattgggcat gttcgaaggc gatatcgacg
    13501 aaggcgaact ggagatcggg caggttgccg gattgatccg ggaagtcgtg ccggccgcgg
    13561 agatcgtgaa agagatcgtg cgtgatttca atttgacgat tgggcgattt gacgattggg
    13621 cgaagggatt gtcgattgtc gatattcgat tgtcgattgt ctattgtcta ttttattttg
    13681 gatttagatt ttgagatttg tttggatttt ggtgcttgga atttggaatt tgtttggatt
    13741 ttggtgcttg gaatttggaa tttgttttgg attttggtgc taacaataaa tttcttacct
    13801 ttaaaccata aacctttatt ctttcatttt gccattgcgt actattgcag gtatccttat
    13861 tttgatcaat ctggcctgct tcggtcagtt ggataattat taccttaaat cctattcggt
    13921 cagcgacggg cttcccaata atcatgtccg ctggatcaca caggaccggg acggatttct
    13981 gtggctggct acctgggacg ggctggcacg gttcgacggc gtcaggttta aaatctaccg
    14041 tcatgatccg aatgattcaa cgagcgtttc tttttttgaa atcatcaaag tagctgtcga
    14101 ttcaggtaac cgcgtatggg tatttgcagg gggcaggtta tgccgttacg accgaagccg
    14161 ggacaacttt atccgttata atttaagcca ttttcccaca ctcggccctg gcgatggccc
    14221 tggggaattt tttgatatcc tgatcgatcc ggaaggctcg ctgctggttg cctgcggatt
    14281 tggcttctac cgttacgatc ccgaatcgga ccggtttgct tcaattcacc ctgccggtaa
    14341 caaagccgga aatttcatcc cttcttttcc cggttttgat aatatgaaca acctttggct
    14401 catgtgggtt gacaaaaccc acgctgacta tgccgaagtt tacaaatgca gttataaaca
    14461 cggacaagtt ttatctgtag tcgactcttt tgtatgcgat ccggtaaatt tccgaagccg
    14521 gtttaccaat gaaacaatcc ggttcaacta ttatctgtca tcagccggag aagcctggat
    14581 ggcctccagc cttggtttat attatcttca gaatgatacc tttaagctga gtcagaataa
    14641 gatacccaag ggacattttt cgaataccgg aatgatcctg tggtcggggc ataataatgg
    14701 gttgatgatt gattatctgc cagaaaatca tgtggataca ctcttcgaag atggaagaat
    14761 tgaaacagtc atagcttatc accatgatga actgggaaat gtatggttca gtgatatgag
    14821 cgaaccatct gtgaaaaacg gtttaagtca ttgtttccgt accggcaatt atttcaggca
    14881 ctaccttacg ggtttggaag gggatgagcc ctacgttatt tatggattat acaaggaccg
    14941 ggttggcaac atctgggccg gcggacgtcc gaatacacat attgtaaaga tttccccgga
    15001 tggcgttgag caacaaatca acctcccatt ccatccggtc aaatatttta atgtgccccg
    15061 aaatatagag cctgatctca acggacacct ttggatcgcc tttttcagcg attacttgtt
    15121 cagccttgat cccggcacgc ttcagtacac agatttctcc ggcatcaata tgacaaaccc
    15181 taccaatctg tcgaaaataa attaccggct ggtaaaaccc tacgggaggg atctgctgtt
    15241 ttctgccggt gcaggcaagc tatacaaagt caataccctg acaggaaacg tcattacaag
    15301 ttcagcacca aacgggtggg atattttttg cctttacccc gttggggata acaccctttg
    15361 ggtcggcctg agcggcaggt tactgttaac cgacttcggt tttaataaac aggaaataat
    15421 tccggtcatt gatccttttt ataacattga agatatctgt ccgggcgact cttcctatct
    15481 ctggctcgcc ttgctgggag ggggtatttg ccgctttgat acattaaacc atcaaacttc
    15541 attttttaca acatttaatg gcctggctca caataccgtt tatagcataa ggaaagatca
    15601 ggccggaaat ttatggataa gtcataacct gggaatttca atgtttaacc cggttacaaa
    15661 aagcttcacc aattatgatg aaaaagacgg gctgaagatt aaggaattca attcggaagc
    15721 cgcttgtcag acagaccagg gcgaaatcct gttcggagga ataggcggaa ttgtcagttt
    15781 tttccctgac agcatcagga atcaaccgga tgatgaccat tcgaaattaa tgatctctga
    15841 tttcaggatt gccaataaag tgtacccttt cgataaacct gtcagcgaaa tgaaaaaggt
    15901 caggttgccg aaagggacgg ataatttcca gctcgaattt attaaacccg atttcagaaa
    15961 tggagatgag atccgctacc gatatacatt gggtggacga atggacaaat ggaccgagac
    16021 cgattgcgac catcgccggg tcaattttac aagccttcgt cccggcgatt acgaattcat
    16081 tgtagaatgc accgatagca acggcgagtg ggcaaatagt acaatgctac tcatcactat
    16141 ccccccctat ttttaccaga ctatagtttt cagactgttg ttagctattg tagtcatggt
    16201 ggtactcggc ctgttttttt atatgaaact caaacagatc aggctcagtg agcataaaaa
    16261 acaggagcag ttacgactgg aaaccttacg tggccagatg aatccccatt tcatctacaa
    16321 ctctctgaat tcgatcaact attttatttc acttaacgac aggctgaatg caaatcaata
    16381 tattaccgat ttttcgaggc tgatgcggtc tattatgacc aattccgccc aggattatat
    16441 tacccttgaa tcggaaattc aggctattaa agactatctg gccctggaac accttcgctt
    16501 cagcaataag ttcgattatg agatcaaaac ggatgaagca ttggacctgg tcatcaccga
    16561 ggtggctccg tcgctggtcc aaccctttat tgagaacgca atctggcatg ggctcaggta
    16621 tatcgaaaat cgtaaaggat tcctgtcggt aaggtttatc atggaaggaa acggcaatct
    16681 ggtctgttat gttgatgatg atggaatcgg ccgcaagctc tccgttaagc taaagaccgg
    16741 ggatcagaaa aagagaaatt cgagaggcat tgccatagtg acagaacgac tggcaattat
    16801 taactccatg caaaaatcaa gatattcggt ccgggtcatg aatatgtatg atgaaaggga
    16861 tgagaccggg actaaagtga ggatagaaat accattcaga agatcattat gataaagtgc
    16921 attattgtcg atgatgaatt tcccgccagg gagtgcatca aaaattatct gaaagattac
    16981 tgccccgatg tggaggtagt ggcccatgca gattcggcca acagtgctta caaagaaatc
    17041 ctggatcacc atcccgacct gttgtttctc gatgtggaga tgccgaacgg cagcgggttc
    17101 gatctgctca gaatgatgaa caagattgac tttcatatca tttttgtaac agctcatgaa
    17161 gaatatgcgg tgatggcttt tcgcttttcc gctatggatt accttctgaa accgatcaat
    17221 atcaaagaac tggtgacggc aattgataag gtcaggacgg agatcagcag gaaatcagaa
    17281 gcagtcaacc tggaaaccct gaaaagaata acgcaaatgg gtcctgccga atgggaccag
    17341 atcgttataa aacatagtga aggatttaaa gtggtttatc tcagggatat cataagttgt
    17401 gaagcggatt cctattgcac gatctttcat ataagtggta ataaaaagct tgtcagttca
    17461 aagaatctga aatactacga agatatcctt acccctcatg gctttcagcg aatccataat
    17521 tcattcttga tcaatttatc gcatgtcgtg gagtattcca gcgaaggggt ggttgccctt
    17581 tccgaaggcc agttagccca tttgggtaat acctacaaga aaaagtttat ggattatttt
    17641 gaaagaaaca agtgaagtta atcgctccat aagaacccta tcattagcta cattcaatta
    17701 agaaaaaatg gtaagaggca taattctatc gcctcaatgc accattacat tagttcatgc
    17761 tttgcattgg tgagattatc ctgaaaaatc atttatgcat ctataaattt aatctgtcaa
    17821 attaacattc aacgtgaacc tcatttgccc cagttgcggc caggctggaa atgaaacgat
    17881 attccggaaa attggccaga aaaagcttct ttgcacaaat tgtaaagcag tgctgcagat
    17941 tgcattgaaa tgcccggcat gcgacggaat catcgaaatg ccattcattt ccgactttgc
    18001 attgtcaaat attgggtcaa ttctcttctg gaattaccgt aaagggctta attataaccg
    18061 tttgacctgt ccggtgtgct cttatatggc aaaaagacag gaatttgtca gagttgccgt
    18121 ttttcatgaa gaatattcga taattatgta atcaacaccc tggcaatgat gtggaatata
    18181 aacagaagcg cactgatcat cctggcggca ctgctaattg aagccggtgc ccttaccgcg
    18241 caaaataaca accaaaatga caagaacttc tttattcgtg gctatgccgg ggcacagggc
    18301 aaaatttcac tgatatttcc cgtagaacta acggagtaca cgcaggactt ttacgaccat
    18361 ttgctggatg agtggggcaa ttacggctat tttcccgaca tgatgaatat tcccccggtt
    18421 tactccggtt acgggtacac cctgaaagcg gggataaggc ttttcaatgt gttccaggtg
    18481 gagccctggt gggagatgta caaatcattc cctttgcaga tcaggctgga tgcttattat
    18541 tatgatggct acagcggtta tagccgggag atcgatatga attacaagtt ccgcccgatg
    18601 tacgatgccc tgggcgcttc gctgatgttt gtccccggct catccgacaa gcctgtgttt
    18661 tttacgttcg ggggcgggat tggaagttat atcggcaagc tgagcattga aaccgacggg
    18721 tcggaaacga taaacgataa caccacaccg gtgtatgatt atgaagaata ttcgggaaaa
    18781 gcgataggtt atcacgcatc tattggtatg acctatgtcc cctggaaata cctggagctg
    18841 gagacattac taaccggccg gtacgtgaaa atccccgaaa tggaaaatgc cgccggctat
    18901 gtattgacca actcatacga tgataaccag ccggtggtca tgcaactttc gggcattgac
    18961 ttccggttcg ggatcaagtt tatttttccg taaatctggc ctttggcatt tgcggtgagt
    19021 tgaaacagcc tctatcgggc gacatcataa tccaccagca ccgctttcac aaacagcgct
    19081 ccgtcgaggt acgtttccca gcccaccatc ccgatgccgg gaatgtaata gtcgtatata
    19141 tcaaagcttg agtatttctt ttcaatttcg tccggaaaat cttcttcatt catcctgaga
    19201 tagtcaaaag ccaggctgga tttatatttc ataggaaggt aaaagttgta cactatacaa
    19261 tccgtgtagc ccccgatgct cgtatttgtc gacaggcagg ttaccgaggt cgggtatgga
    19321 ggcgccaccc agcttgtaac ccaggtatcg ttcacttcca ccgggtattt gtacctcagg
    19381 tcgggcggct gctggtatgg gccgtagccg tagcaccagc aaccgtcgga acgattaatg
    19441 caaagaatac ttaatatcag actattaaac ttctggggcc tggatttata gatcaaagat
    19501 agttgcgtgt acaataattc caattgaaat taaattgctc tttatacttt ccccattcac
    19561 gaccgaaata agcccgatct tatcctgtag atcaaggctt aaccctccct gaaggaatgt
    19621 gaaaccaaca ccgactacat attccggaaa caacacgcga tgtccaacct ctgtccgata
    19681 agtattattt ccggatattg tagccagata gagttcattc gcaaaaagaa tatccagatt
    19741 gatgcccgta tagatataag gagtcagggt gggtttcagt atattatatt taaaatacat
    19801 ccccaattgc aaaccataga ctcttgcttt atatttatca ttctcgaata tctgcttagt
    19861 gccgattacg cctaatccgg catcaacgaa aagaactact ttttcatttt cagataatgt
    19921 cgcgtatgcc tggtaagtaa aactagtaac attgaaaata ggcgtaaatc ccgtactatc
    19981 aatggcatca ccataaaatt tgcccattgg cagatgtacc ttcaaagcgc tctgactgaa
    20041 tgcattgata gatagaatca aagaaaaaat gatgcctata aatacccgca ttccaaaata
    20101 aaattttcta aaaagaaaca attctgattt cataattttc tcctttatga ttattggttt
    20161 taattgctca caatcacaaa ctccaccctc cggttaaagg cccgcccttc ctccgtatca
    20221 ttggtttcca ccggcctgga ttctccgtat ccgacaaagc ttatccttga aagggcaatg
    20281 cttttattct tcaggtattc ataaaccgcc ttggcccggt cttccgacaa tttctggtta
    20341 taggcatcgg tacccatatt gtccgtatgg cccgcaatct gaatgacgat ctgcgggttc
    20401 tcattcatta tatttaccag gcggtcgagt tcaaagaatg acgcttcgag taattccgtc
    20461 ttgttataat caaagaaaat gttatttagc ctgatggtgg ttcctgtatc aataggtgca
    20521 agatacaggt ccctggtgat ttcagaatat tcctttagcg tcgtcaggtc gatattctca
    20581 cttaccgaaa aatatccctc tttccgtgca taaaatgaat aggcatgccc tgaaggcagt
    20641 gaaattttat aggtgccgtc gtccggattc gatctggcaa tgccgatttc ttcatttgtc
    20701 tcaagatcga aataggtaat gctggcttca ataaacccgg aggatttcag gttatacacc
    20761 ttcccggtaa taagggccac cggattgggt ttagccgcct ccgaaacttt tatcctgaag
    20821 atatcttcat ttccaaatga attatcggtc gataccaggt aagaataatc gccggaggcg
    20881 ggaatggtaa aataggcgtc ccatccgggc gtattgatgc tgggccccag gttctgcggt
    20941 ttgctccatt tggtccaggt atcgtcgagc cgccgggtga tgtaaatgtc gtgggatccg
    21001 tatccgggat aggcattgga gctaaagtaa agcgtgacgt tatccgccgc tatgaagggg
    21061 gtaccttcat ccccgaaagt attgatatcc gatcccatat tcaatggctc cgaccatgaa
    21121 tcatcatcct gccggaaaga gatatagaaa tctttttcac catatgaatc tttcctttcc
    21181 agcgacagga tcatttcgtt ctgggagggt gacaggttac tcgaacaata aaggttatcg
    21241 ttgtaatagt ttttaatgtt caaagccgtg gggatagtcc agccgccttt tgtccgctcc
    21301 gatttcgata atcccgcgcc gatcgattta cctgacttgt catagcggtt gatcacaacc
    21361 agcgagttat tatccgggga acagtatact acactgttgt tgctctcatt gttcagcggt
    21421 ttcttgatat tgacgcgtct gctccaggta gaatcaggat tcagtgtgga ataccatatg
    21481 tcctgcgtat cttccttgcc aatattattc ttatcacttt ctaccgtcag gtaaagggtt
    21541 tgcccgtcgg cagagataat cggggctagt tcttccgctt tggagttgac attctcgccc
    21601 agattttcct tcaaaaatat cttgcccgac tcttcgacca gattgatatt ctgccagcca
    21661 tcctgttttg cgacaaaata gtcgaccttc acgttttgat atccttcgtt cataaatccg
    21721 acgattgaga tgcaaaaatc gaaatcggaa ctgctccaga ctttatgatc attgatgaaa
    21781 aactccacca cgccgttcgt atttctgagc tcaagctggt tatactcacc cgggcctttt
    21841 acaataccgg ttttggtagt ttttggcagg acttctacgg gtttatacct gtactgcacc
    21901 tggtgatcga tagcatatcc gtctttcgga tatatcttga atttataaat gcccatttta
    21961 tctgtatagc gcgtgtcaag ggccattaat ccgaaaggct tttttgtatg cccgtcagca
    22021 tactttatac tgcacttaat ggaaaaatcc ttccccggat caatcctgat ctcccgcatg
    22081 accaatactg tttctttcct gagataaccg tgaagaatca gctccccgtt tttgaattcc
    22141 tccacataat cgtcggtact cacccagttc tcttcttttg tatcaaatga atcttcataa
    22201 aggacttcct gagccagaac ccaggttgtg gtgaagatga gcagaaaaaa cagaatcaaa
    22261 gttttcataa tattcagggt ttacagatta ttgaaaagaa ttaatcaaag atcaacctaa
    22321 ggaatcccac atttctttta aacttgtcgg cacacaggta aataacttta ccgggatcag
    22381 gagaataaag ctcaggaaac tgatcatcaa caaagaaaga ctcacttcca taaattctaa
    22441 aagcggaaat ttcacccgta gtcatatcaa ttttaccgat ggtgggatat tgcaacaaat
    22501 caccctccca catggagctt gtttcaagta taacccaata taagtattga tggcccagtc
    22561 cctcaatgaa aaattgtcct gttccgtact ctttggcgca ggagttgttt tcccttgtat
    22621 caagcccgta ttgtgaaaca agttgtccct gaggattaaa ataaaacgcc agcacatctg
    22681 tgtatacgtc ttttgatgtg ccggcgacag gattgaaagt ggtggaccag ttttgtccgg
    22741 caacaaagaa tttcccgtca tcggccacat agtatgcatc tttctcgaat ttattcccgc
    22801 ggtatgcggg aagcttttcc tgaccgggcg gtacctggag tttttttgtg taatcatctg
    22861 tcagggtagc ttcaatatat tccacctgtc cattcttgac tttcatcagc tgagtcgctg
    22921 taaaatcaac tcccacaaaa gggttgccca taccaaactc acctcctgct gcagcaaaat
    22981 aataggtgac ttcacggttg tcagcaggcc caaaaaaata cgcttcctct ttgctttccg
    23041 gtgcaatcat atcatcaatt ttccagaatg aatagtgcga taaaatgggt atctccccgg
    23101 taacttccag tttattgttc atttgtacaa acttaaagtc gaatgcggtt ttgctgaggt
    23161 cttctttcgc cggtccgaat atgagataat actcttcaaa tccttcttca tccgtattca
    23221 catcaatccc ttcacaaaac gcaagatatt gctgcttatc gaaactcaaa ctgcatgatt
    23281 tgacaatccc catattgcgg tccagttgta taatgttaat atcattg
//
LOCUS       DAOPNH010000010        23187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2032_length_23187_cov_6.974754, whole genome shotgun sequence.
ACCESSION   DAOPNH010000010 DAOPNH010000000
VERSION     DAOPNH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_23187_cov_6.974754"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKZ74_01300"
     CDS             complement(<1..250)
                     /locus_tag="PKZ74_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91414.1"
                     /translation="MRQKKYVNILIVIGASAILLLLWFNGFSWLYAQMLRIGANILLV
                     FSGDTSISLKMVDNAPTFVVDTIINGKKGTYPQKADLIL"
     gene            complement(228..884)
                     /locus_tag="PKZ74_01305"
     CDS             complement(228..884)
                     /locus_tag="PKZ74_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPM91415.1"
                     /translation="MKNQKTTRNKPEPAKSTGKSVKGTNQPKKGNAAGPKKPLFSKEL
                     LLKLMPLIKAFALWFLLVLIVHIPFIKDEFRNMFVGFTTNSTVLLSKILFIPIQKLGF
                     STISLDGFTMDIVVECTAYNFYLFAIALSVFGNWSLKHKLINLLIFFGIIFLTNNLRF
                     FAMGYIGRYYPELFDTTHDYVWNILFGFMIFGVWAWRDKKNNPDFNPKDPDHATEEVR
                     "
     gene            complement(1098..1565)
                     /gene="pyrI"
                     /locus_tag="PKZ74_01310"
     CDS             complement(1098..1565)
                     /gene="pyrI"
                     /locus_tag="PKZ74_01310"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPM91416.1"
                     /translation="MEEVKTRTELQVSAIENGTVIDHIPPKSVFQVVKMLGMENCKEQ
                     VLFGTNLESKKYGRKGIIKVANLFFQSDEINKIALVAPSATLIVIRNYQVVEKRKVEI
                     PDQIFRFVRCFNPNCITNHEKITTKFNVIDKEDLKLECIYCEKVTGKNNLEFI"
     gene            complement(1603..2511)
                     /gene="pyrB"
                     /locus_tag="PKZ74_01315"
     CDS             complement(1603..2511)
                     /gene="pyrB"
                     /locus_tag="PKZ74_01315"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPM91417.1"
                     /translation="MKHKSLVSIDDYSKEEQIQILDLAEGFEAKPVQNILNGYVVATL
                     FFEPSTRTRLSFESAASRLGAQVIGFSEASSSSVSKGESLNDTILTVSNYSDIIVMRH
                     PREGSARYASEVSPVPIINAGDGANQHPTQTLLDMYSIRKTQGRLDNLQIAFVGDLKY
                     GRTVHSLTFALCNFNAAFHLVSPEELKLPSYVKRKIKDNKLDYFQYTDMNEVIPQVDI
                     LYMTRIQKERFSDPIEYERVKNAYVLRNEMLQGAKKSMRVLHPLPRVNEIEKDVDPNP
                     MAYYFQQAQNGVYVRQALLASILGVC"
     gene            complement(2536..3873)
                     /locus_tag="PKZ74_01320"
     CDS             complement(2536..3873)
                     /locus_tag="PKZ74_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPM91418.1"
                     /translation="MKKNVIIIGAAGRDFHNFNTYFRGNESFNVVAFTAAQIPDIDGR
                     KYPAELAGHLYPDGIPIFAESNLPELIKELKVDICVFSYSDVTYQKVMAISAIVNSAG
                     ANFLLLGPRDTMVKSTKPVIAVGAVRTGCGKSQTSRRIIEILMAKGLKVVAVRHPMPY
                     GNLVEQKVQRFATIADLAKHKCTVEEMEEYEPHVVRGNVIYAGVDYEAILRAAEQDPD
                     GCDVVLWDGGNNDFPFYTPDLNFTVVDPHRPGHELTYYPGEVTLRLADVVIINKIETA
                     EPKSIQVVRENIAKVNPKAIVVDGASPIKVDNPSVIKGKRVLVIEDGPTLTHGEMKIG
                     AGTVAAQKYGAAEMVDPRPFTVGKLSTTFKIYPNIGTLLPAMGYGEQQLKDLEETINA
                     TDCDSVVIGTPIDLNRIIKINKPNTRVYYDLQEIGDPDLNSILDEFVKKHGLK"
     gene            3996..5786
                     /gene="argS"
                     /locus_tag="PKZ74_01325"
     CDS             3996..5786
                     /gene="argS"
                     /locus_tag="PKZ74_01325"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPM91419.1"
                     /translation="MIEKIIAEKASDALKSLYGQNIDPKGVAIQKTRPEFQGDFTVVV
                     FPLTRFSGKSPEETGRELGEWLKGNFNFIADFNIIKGFLNILLTDRYWMDFFSTGSLD
                     ENFGFFPQSEGPPVVIEYSSPNTNKPLHLGHIRNNLLGWSIAEILKANGRKVMKVNLV
                     NDRGIHICKTMLAYRKWASGKIPSKEGIKGDKFVGNLYVQFEQANKKIMETIIKQGID
                     EDEAYRQSGLTQEALDLLQQWESGDPDARRLWETMNGWVYEGFDQTYRRLGVDFDKIY
                     YESETYLLGKDLVAEGLDKGVFYKKDDGSVWIDLKDFKLDEKLLLRPDGTSVYITQDL
                     GTAQLRVDDYHPGRLLYVVGNEQIYHFDVLKIVLKLLGRDWAENILHVSYGMVELPQG
                     KMKSREGTVVDADELMEEMYETAKKTTAELGKLDNPDDPEWRELFEMLGMGALKFFIL
                     RVDPRKNMLFNPQESIDFNGHTGPFIQYTHARIRSLLAKASGKGYKAITSPLPDDFAL
                     LPKERAIVRLLYDFPAVVADAGNNLSPAIIAAYCYELAKEYNQFYQEIPVLKEPDPLK
                     ISFRLALSGFAGNVLRRGMGLLGIRVPEKM"
     gene            complement(5795..6739)
                     /locus_tag="PKZ74_01330"
     CDS             complement(5795..6739)
                     /locus_tag="PKZ74_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPM91420.1"
                     /translation="MFKRFSSVILMAILAGLLIVYFIVRFSGTNDRTFRDKVLSFDPA
                     VVTSITIDDPKSMQEPVELRLSGEKWMVRSGVKEYPADSNIVKGALNTLSDMPTKRFA
                     GKGKESWIKYEVTDTSGIKVTLKSSSKTVAEILIGKFTFNMPKDQQQSQFRQQRGDMT
                     SFVRLADEKDVYAVDGYLRTTFSAKPDSYRYKNLSGVNAQDITKMTVSKPGGLTVYDL
                     STGKWTMNGMPADSATTARYRSAVARLSGSKFVDPEMVRSAPAYTLRLEGNNFSPVDI
                     MAYPVADTNIAYAISSSVNPDAYFDGKTGGLFKRIWPD"
     gene            complement(6732..8255)
                     /locus_tag="PKZ74_01335"
     CDS             complement(6732..8255)
                     /locus_tag="PKZ74_01335"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPM91421.1"
                     /translation="MKIKGAKSMTTKILLMTGIVIVINILSDQLYFRLDLTADKSYTL
                     SKATKNILRSLEEPVTVTAYFTEEVPPQLLQARKDFKDLLIEFSNVAKGNLVYEFIDP
                     NKSEELERKAMQEGIMPSIVNVRERDQMMQKRVFMGAVLKMGSEKEMIPMVQAGSAME
                     YDLASSIKKLSVTDKPAIGLIQGHGEPSMGALQQVMASLGILYSVRPVDLENPSDDPG
                     KYATIMMVAPTDTIPAHHFDVLDRYLAGGGNLFIAYNKVKGDFTTVQGTSNYTGLEDW
                     LAKKGLYIDNNFLVDATCGSVGVQQQAGFMSFTTNVSFPYLPVIQKFADHPVTKGLEQ
                     VMLPFASTIQFTGDTSLAFTPIAFSSQKSGTLNPPLYFDINKKWTDRDFPLSGLTVAG
                     ILSGRMGGMAESKIILVGDGDFPVNGEGQRPQQLQPDNVNLMVNAVDWLSDETGLIDL
                     RTKAITARPLKDVAEGRALFIKWLNFLLPIILIILYGIFRNQRNRSLRVKRMEEGYV"
     gene            complement(8291..9013)
                     /locus_tag="PKZ74_01340"
     CDS             complement(8291..9013)
                     /locus_tag="PKZ74_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPM91422.1"
                     /translation="MNQIWTIAKRELGSFFDSLMAYIMMVAFLGFSGFFTWIFGTDIF
                     FIKQATMQPFFGIAYWTLFFFIPAITMRLFAEENKSGTIELLLTRPVTDWQVIAGKFL
                     ATFLLILIALGLTLPYYITVASLGPVDHGSVITGYIGLMLISCAYISIGLFASSVSNN
                     QIVAYLIALVIGIFFHIIFDLLASNLSGLPGEIISFLSISQHFESVSRGVIDSRDLIF
                     FFSVIFISLILTEMNILKKRTA"
     gene            complement(9056..9985)
                     /locus_tag="PKZ74_01345"
     CDS             complement(9056..9985)
                     /locus_tag="PKZ74_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM91423.1"
                     /translation="MDILVENLTKIYGTQRAVDNITFRVKTGEVLGFLGPNGAGKTTT
                     MKAITTYLVPNEGSISVGQYTLKDQAEEIKKHIGYLPENNPLYQEMPVIDYLKYVAEL
                     QGIEKPRIRKRILEMVRLCGLEGEKHKNISELSKGYKQRVGLAQALIHDPDVLILDEP
                     TAGLDPNQIVEIRELIKKIGKEKTVILSSHILAEVEATCDRILIISKGKIVADGTAEE
                     LRKRAHGKEILKVRIEDGEANTVFTALKNLDTVELVDFIKNEESSYEIQSKDGVSSRR
                     AVFKLCVSKGWTLTGMSSIETKLEDIFRELTMN"
     gene            complement(10102..10662)
                     /locus_tag="PKZ74_01350"
     CDS             complement(10102..10662)
                     /locus_tag="PKZ74_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPM91424.1"
                     /translation="MKKVIFTGIALFAAVLFVLPAKSQSQAAPSADKIKWMDFEEAVA
                     MNQKKPKKIFIDMYTDWCGWCKKMDASTFVNPVIVKYMNDNFYAVKFNAERKDEVTFK
                     GKNYVNGNPTGSRSSHQLAQELLNGRMSYPSFVFMDESLNKITILPGFQKAPQFEAVL
                     HFIAKDIYKTQKFEEFRAGFQTTAVE"
     gene            complement(10722..11180)
                     /gene="smpB"
                     /locus_tag="PKZ74_01355"
     CDS             complement(10722..11180)
                     /gene="smpB"
                     /locus_tag="PKZ74_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPM91425.1"
                     /translation="MSSSAKTITIKNKKAYFEYFLLEEFEAGIVLTGTEIKSIREGKA
                     SFVDSFCAFENGELFVKNLHISEYTLGTHYNHDPKRDRKLLLNRRELKKLEIKTREKG
                     LTIIPFLLFVNEKGLAKLKIALAKGKHSYDKRETLKNKDIKREIERNKEF"
     gene            complement(11329..13740)
                     /locus_tag="PKZ74_01360"
     CDS             complement(11329..13740)
                     /locus_tag="PKZ74_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91426.1"
                     /translation="MKTGLIFTIWIVLISLLSFGQVDLKGIVSDMETREPLAGAHILV
                     ENTFIAAISSPSGQFRITGLKQGGYRIIVSYVGYEKSSSYIKINKDTTLEISLRPSAI
                     MGSEVIVTATRAANNSPATFSTITSKEIGALNLGQDLPMLLQTSPSVVTTSDAGNGIG
                     YSNMRIRGTDLNRINVTINGIPLNDSESHGVWWVDLPDFASSSESIQIQRGVGTSTNG
                     AAAFGASINIRTDDPEPLPYGELSASTGSFETFKLTFKAGTGLIKGKWAFDGRFSKIN
                     SGGYIDRASSDLSSFALSGGYFGKKSIVRLLMVSGTEKTYQAWDGVPGYLLDSNRTYN
                     GLGAYTDDNGNLKYYDNQTDNYHQTHYHLNWMQTLGSDWNLTTAFHYTRGFGYYEEYR
                     TGQDFEDYQSGNVIIGSDTITSADLVRRRYLDNHFYGYTFGLNYDDKNRVRATIGGAV
                     NYYDGGHYGEVIWAKTPGGFPKDFRWYESESGKLDANIYAKAAWQAAERLNLYGDLQI
                     RRISYTISGIDNDLRDISQDHIYTFLNPKIGGYLTINEHNEAYLSFAVGSREPNRSAL
                     IDANPARPYPVAEHLYNTEAGYKFNSRTIALTANLYFMYYADQLVLTGRINDVGDPVM
                     ENVDKSYRAGIETSLALNLAKNLRWDVNATLSMNKIVDFTEYVDNWDEWPEQIIIRHR
                     TTDIAFSPSVIAGSSLGWKPVKDMQITLISKYVGKQYIDNTSSDERKLDPYFTNDILL
                     NYTISLKSLKQLGFSLKINNITNTEYESNAWVYRYYSEGNYGVYDGYFPQAGIHFLAG
                     IELKF"
     regulatory      complement(13885..13979)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14061..16529)
                     /locus_tag="PKZ74_01365"
     CDS             complement(14061..16529)
                     /locus_tag="PKZ74_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM91427.1"
                     /translation="MIENPWIRGDGCSLVTNVLNLFPNQVGWGFPNILVDYLLRFEWT
                     GETCQGYPIHFKPNFIPTPQTIQWNFDDGPGSNSWQLSPTYTFKNPGVHEVKVDVWYP
                     SGRYEHTSREIEILPSPQPDLGPDTLICKDALLVLSANCEADFFSWSTGQFGVSSITV
                     SDSGTYWVRGRFNNSGCMGYDTIHVGFHAPTIIDETNLIITQTTCNGASGSITGLIAL
                     GYSPFAYRWKDLSEVQVGTDIDVSGLPAGQYYLTITDANGCETVSNVYTIEDAGNLQV
                     LNVEQTRPHCGRPDGQIIIHALSPAGSALQYSIDDGDSYQTDSVFSGLIGAGYVVRVT
                     DGVGCFGFYNDNPVLLDDIPGPQVVDVIVTDETDFLGNGAIEIVATGSTPVIFYSIDS
                     GATYQLNNGTFNDLGSRIYNLQVWDENGCDTTFTVEIQNIILTWLHAVTGEGGHCLGN
                     TAMVPVNVDNFNAVADFHLKLGYNAGHLECEGFANVNVQLLDSLTGWVDAIAGDIHLT
                     WNGESPLTFSQPEKVADLVFTTKNPGQGELSWYTGEAESYFTNSSGNPIPAEFSTGQV
                     TIYQPPQIILDQSKTVCTGQFVSLMSIAVGNQNPLTFRWIYPTGDSTSTDPFFFSVTP
                     ADAGLYTLLATDRVGCTDRKSIELIVSENPVAAFHGTDTLEMHAGEVLDAGEGMASYK
                     WNTAYSTQSIVIQAEGKYLVEMESQIGCIGRDSIYIKLSFEEIPDFKIFIPNAFSPDG
                     DGINDSFQIKLTNSTFNIQHSTLRIFDRWGGEVFFGDGISLGWDGKKNGKECPGGVYV
                     YKIVFSIDGVPGSQERAGTVMLVR"
     gene            complement(17598..18026)
                     /locus_tag="PKZ74_01370"
     CDS             complement(17598..18026)
                     /locus_tag="PKZ74_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91428.1"
                     /translation="MKRLIHIIAVISVIFFIASQSLNAQITIKVTLDGQCPDTCTSHE
                     ICTYKVEYQLYNICVEPPELCCTAPPQWVNCSTEEIYFDCDFSCDDPSNEKCYALVGI
                     ATKYCNGPGGNVAKCTGNGVHYYTCNELMTLPNVPVTVLW"
     gene            18414..18839
                     /locus_tag="PKZ74_01375"
     CDS             18414..18839
                     /locus_tag="PKZ74_01375"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPM91429.1"
                     /translation="MNLFIINGPNLNLLGKREPEIYGNQSFDEFLDILRAEFPGHSIG
                     YYQSNTEGEIINAIQDAGFTTDGIIINPGGYSHTSVAIADAMKAVPARVIEVHISNVF
                     QREDFRKVMVTATGADALICGFGLESYRIAIRNLTGDSL"
     gene            complement(18820..19848)
                     /locus_tag="PKZ74_01380"
     CDS             complement(18820..19848)
                     /locus_tag="PKZ74_01380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91430.1"
                     /translation="MKTAVVILNWNGREFLEKFLPGVIAHSAGEAEIIVADNDSADDS
                     LAFLEAAHPGIRIIRLDKNYGFAGGYNRALKQVDAEYFILLNSDIEVTENWITPVIRL
                     MESDASIAACQPKIRSYKEPEQFEYAGAAGGFIDKYGYPFCQGRLFQHIENDEGQYDQ
                     AREVFWATGACLFVRSVVFTQLGGFDDDFFAHMEEIDLCWRAKNQGYKIMYCPESTIF
                     HIGGGTLPKKNSRKTYLNIRNNIIMLYKNLEPGRLLSVMLARFILDGVAAMKFLVDGG
                     FKDMFAVFKAHIYFWSHLYLLRKKREKIPHHRVSEIYWGNIVVEHYLRRKKSFGQLDP
                     ERFTKNPR"
     gene            complement(19905..20786)
                     /locus_tag="PKZ74_01385"
     CDS             complement(19905..20786)
                     /locus_tag="PKZ74_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM91431.1"
                     /translation="MAFLYHTFKLLANLVSRLPFPVLFFVAGIFNFFLHYIIRYRRDI
                     ILKNLRNAFPEKKPGEIRKILGQYYKNLSQIMFEVIKLHSIRPEKLLERFTFTGLEPL
                     AETLRKGQSVIITIGHCGNWEWMGTALGQVLPARGFAIVKPLSDKHFNRYMESLRHRI
                     NPDSTIPFHRTYRTLIRHKQEMVTFNVFAADQTPVKEEANYWTRFLNQDTPFYIGVEK
                     LAKALHLAVFFIDIQRTGNGKYNGEIRQITREPEKTAEKEITEAYIRLLEEAIRRRPD
                     NWLWSHRRWKLTRTPGN"
     gene            complement(20848..22320)
                     /locus_tag="PKZ74_01390"
     CDS             complement(20848..22320)
                     /locus_tag="PKZ74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPM91432.1"
                     /translation="MKRILFVAILINTLLSPAVMQSKTLQAYLNYTVFNVPGKGPFIE
                     TYLSVEGKSVFYMPNESGQFQGKIEVTFIFRQGEEIKEFDKYQLFSPVVSDTVAIDFS
                     FIDQQRYFIPEGEYEMEIMIADLNTGKKPFSATQPVTINFDDKAACFSGVQFVKSFSP
                     SSQQNILTKGGYDLVPLVYNFYPESTNKLIYYTEIYNSDKALGPGEKFLNVTMIRSFE
                     TENTLKEFVTYRKFDAGPVVPVFGEFDISRLPSGNFLLVFQVKNRQNEVIAENKMFFQ
                     RSNPNIQFSLDDLATIDVNATFASRITNADTLQEFINCLDPIATELEKNFIYKQAKLA
                     DLKTKQQFFYNFWSSRDMINPETAWKDYYGQVQVVNKAYKTQISKGYDTDRGRVYLKY
                     GPPNIISENYNEPSSYPYEIWHYYELGGNQRNKKFVFYTQNIITNNFQLLHSDAIGEI
                     QNYKWQVFLNNRWYDPYNVDANQAPGIYGGKADDYYRNPR"
     gene            22488..22576
                     /locus_tag="PKZ74_01395"
     tRNA            22488..22576
                     /locus_tag="PKZ74_01395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22522..22524,aa:Ser,seq:tga)
     gene            22581..22669
                     /locus_tag="PKZ74_01400"
     tRNA            22581..22669
                     /locus_tag="PKZ74_01400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22615..22617,aa:Ser,seq:cga)
     gene            complement(22813..>23187)
                     /locus_tag="PKZ74_01405"
     CDS             complement(22813..>23187)
                     /locus_tag="PKZ74_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91433.1"
                     /translation="GESVDMGAYEYGPWVNVPSAPGSRFQVPGSKLLNVNPNPFIFGT
                     YISYELKSAGRLNISIFNSSGMLVKTLVNFKGGTEETGEIYWDGAGPGGTALPAGIYF
                     VRLTIDGKETETVKVVKVENSH"
BASE COUNT         5834 a         5322 c         5593 g         6438 t
ORIGIN      
        1 gcaggatcag atcagccttc tgcgggtagg tccctttttt cccgtttata atggtgtcga
       61 caacaaaagt aggggcgttg tccaccattt tcaggctgat actagtatcg cccgaaaaaa
      121 cgagcaggat attggcaccg atccgcagca tctgtgcata gagccatgaa aatccgttga
      181 accagagcag cagcaaaatg gcggatgctc cgatgacgat caatatatta acgtacttct
      241 tctgtcgcat ggtcagggtc tttggggtta aaatcggggt tgttcttttt gtcacgccat
      301 gcccacacac caaatatcat aaatccgaat aaaatattcc atacatagtc gtgggttgta
      361 tcaaaaagtt caggataata tcttccaata taccccatgg caaagaaacg caggttattg
      421 gtcaggaaaa tgatgccgaa aaagatcagc agattgatca gcttatgttt cagcgaccaa
      481 ttcccgaata ccgacagggc aatggcgaac aggtaaaaat tgtaagccgt gcattcgacc
      541 acaatatcca tggtaaaacc atccagtgaa atagtggaga atcccagttt ctggatggga
      601 atgaacagga ttttcgataa aagtaccgtt gaatttgttg taaatcccac gaacatattc
      661 ctgaattcat ccttaataaa aggaatatga acgatcagga caagtaaaaa ccaaagggca
      721 aaggccttga tcagcggcat aagttttagc aggagttctt tgctgaacag gggtttcttg
      781 ggcccggcgg cattaccctt tttcggttgg ttcgttccct tgacggattt gcccgtcgat
      841 tttgccggtt ccggcttatt cctggtggtt ttttggtttt tcaaggcaaa ataattttgt
      901 tgtgtaaaaa tataaaagaa tgcagaatta caatgaggga ttttgtaatt atatcgaggt
      961 tttaatattt atcgcgtatt acctttttta ttaaaaatga taaataccgg atggtggttt
     1021 aaaaaagcgt accgggccgg cggaatttat tccaaaccgg cccgggcgcc ttttaatacc
     1081 ttatgtaacg gaatttctca aatgaattcc aggttgtttt ttcctgtcac tttctcgcaa
     1141 tagatgcatt ccagtttcag gtcttcttta tcgatcacat tgaattttgt cgttattttt
     1201 tcatgattgg tgatgcagtt aggattgaag cacctgacaa aacgaaagat ctggtcggga
     1261 atttcaactt ttctcttctc aaccacctgg taattgcgga tgacgattaa agtggccgaa
     1321 ggtgcgacca gtgcgatttt gttgatttca tcactttgga agaacaggtt ggctactttt
     1381 ataatccctt tccgtccgta ttttttactt tccaggttag tcccaaaaag aacctgttcc
     1441 ttacagtttt ccattcccag catcttcacg acctggaaca cgctcttcgg cgggatgtga
     1501 tcgatcacag taccattctc aatggcactg acctgtaact ctgttcgcgt ttttacctct
     1561 tccatcattc aacattcaac tttcaacatt caattttcaa tattaacaaa ctcccagaat
     1621 cgatgccagc aaagcctgcc ttacatatac cccgttctgt gcctgctgga aataataggc
     1681 catcgggttc ggatccacat ccttttctat ttcattgacc cgaggcagcg gatgcaatac
     1741 cctcatgctt ttctttgcgc cctggagcat ctcgtttcgc aatacatacg cgtttttcac
     1801 ccgttcgtac tctatcggat cggagaacct ttctttctgg atgcgggtca tgtaaaggat
     1861 atcgacctga gggatcacct cattcatatc ggtatactga aaataatcga gcttattatc
     1921 cttgatcttc cttttgacat aactcgggag ctttaattct tccgggctca ccaggtggaa
     1981 agcagcgttg aaattgcaaa gggcaaaagt cagcgaatga acggtcctgc cgtatttcaa
     2041 atcgccaacg aaagcgatct gaaggttatc cagtcttccc tgggttttac ggatcgaata
     2101 catatcgagc aatgtctggg tcgggtgctg gttggcgccg tcgcctgcat tgataatggg
     2161 taccgggctg acttcgctgg cgtatcgtgc actgccttcc cggggatgcc tcatcacaat
     2221 aatatcagaa taattgctga ccgtcaggat cgtatcgttc agggactccc ctttggatac
     2281 gctggaagaa gaagcttccg agaagccgat aacctgggct cctagacgtg atgcggcact
     2341 ttcaaaacta agccgggtac gggttgatgg ttcgaaaaat agtgtggcaa ccacataccc
     2401 attcaggatg ttctggaccg gcttcgcttc aaatccttct gccagatcaa ggatctgaat
     2461 ttgttcttct ttgctgtaat cgtcgatgga gaccaggctc ttgtgcttca tggattgata
     2521 tttatgaatt aaatgctatt tcagaccgtg tttttttaca aattcatcga ggatcgaatt
     2581 caggtccggg tcaccgattt cctgcagatc gtaataaacg cgggtattcg gcttgttgat
     2641 cttgatgata cggttcagat cgatcggggt gccgatcact actgaatcgc agtcggtagc
     2701 attaatcgtt tcctcgaggt ctttaagttg ctgctctccg tagcccatag ccggtaataa
     2761 tgtgccgata ttgggataga ttttgaatgt tgtacttagt ttgccaacag taaagggcct
     2821 cgggtcgacc atttcagcgg ctccgtactt ctgtgcggca acggttccgg cgcctatttt
     2881 catttcaccg tgggtaaggg tggggccgtc ttcgataacc aggacccttt ttcctttgat
     2941 cactgaggga ttatccactt tgatcggtga tgcgccatcc accacaatgg ctttgggatt
     3001 gacttttgca atattttccc tgaccacctg gatgcttttg ggctcggcgg tttcaatttt
     3061 attgatgatg accacatcgg ccagccgcag ggttacttct ccagggtaat atgtcagttc
     3121 atggcccgga cggtggggat ccaccacggt gaagttaagg tccggtgtgt agaaggggaa
     3181 atcattgttt ccaccatccc agagcacgac gtcgcatcca tcgggatcct gttcggctgc
     3241 acggagaatg gcttcataat cgacgccggc atagatcaca tttcccctga ccacatgggg
     3301 ttcatattct tccatctctt caacagtgca tttgtgtttg gccaggtcgg cgatggtggc
     3361 aaaacgctga actttttgtt ccaccagatt gccataaggc atgggatgcc tgacagccac
     3421 aactttcagc ccttttgcca tcaggatctc gatgatccgg cgggaggtct ggctctttcc
     3481 gcaacccgtc ctgactgcac ccactgcgat caccggcttg gtcgacttaa ccatcgtgtc
     3541 tctggggccg aggagcagga agttggctcc ggctgaattt actatggcgc tgattgccat
     3601 cactttctga taggtgacat cgctgtagga aaagacacag atatcaactt tcagctcctt
     3661 aataagttcc ggtaagtttg attcagcaaa gattgggatc ccgtcaggat aaaggtggcc
     3721 ggccagttca gccggatact tccttccgtc aatgtcaggg atctgggcgg ctgtaaaggc
     3781 tacaacattg aacgattcat tgccgcgaaa ataggtgttg aaattgtgaa agtccctgcc
     3841 tgcagctccg ataataatca catttttctt cattccgtat gcttgttaag ttagtgatac
     3901 atattttggt aacaaagata gaattttaac ccgaaccccg attggctcct ttctgaattt
     3961 ttatttgcct atttttgaca atctgaattt atacaatgat tgaaaaaata attgctgaaa
     4021 aggcttccga tgctttaaaa tcgctttacg gacaaaatat tgatcccaaa ggagttgcga
     4081 tccaaaaaac gcgccctgaa ttccagggtg atttcaccgt ggtcgttttt ccgctgacaa
     4141 ggttttcagg aaaatcaccg gaagaaaccg gccgggaact gggtgaatgg cttaaaggaa
     4201 attttaattt catagctgat ttcaatatca ttaaaggatt cttgaatatc cttctcaccg
     4261 atcgttactg gatggatttt ttttccacgg gaagcctgga tgaaaatttc ggattttttc
     4321 cccagagcga agggcccccc gttgttattg aatattcttc cccaaatacc aacaagcccc
     4381 ttcatctcgg tcatatccgg aacaatcttc tgggatggtc gattgccgaa atcctgaaag
     4441 ccaacggacg gaaagtcatg aaagtcaacc tggtgaacga cagggggatc catatttgta
     4501 agaccatgct ggcttacagg aagtgggcat cagggaaaat tccctccaaa gagggcatta
     4561 aaggggataa gtttgtcggt aacctgtacg tacaatttga gcaggccaat aaaaagataa
     4621 tggaaaccat tatcaaacaa ggaattgacg aggatgaagc ataccggcaa tcgggcctga
     4681 cgcaggaagc ccttgatctt ttgcaacaat gggaatcagg ggaccccgat gcacgccggt
     4741 tatgggaaac aatgaacggg tgggtttatg aaggatttga ccagacctac agacggttag
     4801 gagttgattt cgataaaatt tattatgaat ccgaaacata ccttctgggc aaagatctgg
     4861 tagcggaagg tttggataag ggtgtgtttt ataagaagga cgatggttcg gtgtggattg
     4921 acctgaagga tttcaaactg gatgaaaaac ttcttttaag accggatggg acttcggttt
     4981 atatcacaca ggacctggga acggctcagc tccgcgtgga tgattatcat ccggggcgtc
     5041 tgctttatgt ggttggcaat gagcagatct atcattttga tgtcttgaaa atagtcttga
     5101 agctactggg tcgtgactgg gcagagaata tccttcatgt atcctacggt atggtcgagc
     5161 tgccgcaggg caagatgaaa tcgagggaag ggacggtggt ggatgccgat gagttgatgg
     5221 aagaaatgta tgaaacggca aagaaaacca cggctgaact gggtaagctg gataatcccg
     5281 atgatccgga atggcgtgag ctttttgaga tgctggggat gggggcgctg aagttcttta
     5341 tccttcgtgt cgatcccaga aagaacatgc ttttcaatcc tcaggaatca attgatttta
     5401 atggccatac cggaccattc atacagtata cgcatgcacg gatcagatca ttactggcca
     5461 aggcttccgg taaaggctac aaagccataa cttcaccgct tccggatgac tttgcactat
     5521 tgccgaaaga aagggcgatt gtcaggcttt tgtatgattt cccggcagtg gtggccgatg
     5581 cggggaacaa cctcagccct gccatcatag ccgcttattg ctatgaattg gcaaaagagt
     5641 ataaccagtt ctaccaggaa ataccggtac tgaaagaacc cgaccccctg aagatttctt
     5701 tccggctggc gctttccggg tttgccggaa atgttttgag acgcggcatg ggacttcttg
     5761 ggattcgggt tcccgaaaaa atgtaatgtt cctgttaatc gggccagatt cgtttgaaca
     5821 atccgccggt tttaccatcg aaataggcgt caggatttac cgatgacgaa atggcgtatg
     5881 cgatattggt atcggcaaca ggataagcca taatatcgac cggggagaaa ttatttcctt
     5941 caaggcgcag ggtataagcc ggcgccgatc tgaccatctc cggatcgaca aacttacttc
     6001 ccgacagtcg ggctacggcc gaccggtacc tggccgttgt agctgaatcg gccggcatac
     6061 cgttcatagt ccattttcct gtagatagat cataaacggt taaccctccg ggcttcgaaa
     6121 ccgtcatttt agtaatatcc tgcgcgttga ctcccgacag gtttttgtac ctgtaggagt
     6181 cgggtttggc cgagaaagta gtccgaagat agccgtcaac agcgtagaca tctttttcgt
     6241 ctgccagcct gacaaacgat gtcatatctc ctctttgctg ccggaattgg ctctgttgct
     6301 ggtctttcgg catattaaac gtaaattttc cgattaggat ctcagccaca gttttggatg
     6361 aagattttaa tgttactttg atgcctgagg tgtctgtcac ttcatatttg atccatgact
     6421 ctttcccttt accggcaaac cttttggtcg gcatgtcgct cagcgtattc agcgcgccct
     6481 tcacgatatt tgaatcggct gggtactcct taacccctga tctgaccatc catttttcac
     6541 ccgataaccg gagttcgaca ggttcctgca tgcttttggg atcgtcgatc gttatggaag
     6601 tcacaactgc cggatcaaag gagaggactt tatctctgaa agtgcggtcg ttggtgcccg
     6661 agaaccggac aataaaatat acaatcagca atccggccag gatcgccatt aagatgactg
     6721 atgaaaatct tttaaacata accttcctcc attcttttta cacgtaaact tctgtttctc
     6781 tgatttctga agatgccgta cagaatgatc aggatgatgg gaagaaggaa gttgagccat
     6841 ttaatgaaca gggcccttcc ttcagcgaca tccttcaacg gccttgcggt aatagctttg
     6901 gttctgaggt cgatcagtcc agtctcatcc gacagccagt cgacggcgtt gaccatcagg
     6961 ttgacattat cgggctgcag ttgctggggc cgctggcctt ctccgttcac cgggaaatcg
     7021 ccgtcgccaa ccaggatgat cttcgattcg gccattcctc ccattcttcc ggataaaatt
     7081 ccggctaccg tcagcccgga tagcggaaag tcgcgatcag tccatttttt attgatgtcg
     7141 aaatacaggg gcgggttaag tgtgcccgat ttctgcgatg aaaaagcgat tggggtgaaa
     7201 gccaggctgg tatcgccggt gaactggatc gtgctggcaa aaggcagcat cacctgctcg
     7261 agtcctttgg tgaccggatg atcggcaaat ttctggataa caggaagata tgggaagctg
     7321 acattggtcg tgaaactcat aaaaccagcc tgttgctgaa ccccgacgga accgcaggta
     7381 gcatccacca ggaaattatt atcgatgtac aaaccttttt tcgccagcca gtcttccagg
     7441 ccggtgtaat ttgatgtccc ctgaactgtt gtaaaatccc cttttacttt attataagcg
     7501 atgaaaaggt ttccgccacc ggccaggtaa cgatcgagca cgtcgaaatg gtgggccggg
     7561 atcgtgtcgg ttggcgcaac catcatgatg gtagcatact ttccgggatc atccgaggga
     7621 ttctcaagat ctaccggccg gacggagtac aatattccca gcgacgccat tacttgctgc
     7681 agtgcgccca tggaaggctc gccgtgcccc tggatcagcc cgatggccgg tttgtcggtc
     7741 accgaaagct tcttgatcga agaggccagg tcatattcca ttgcgcttcc ggcctgtacc
     7801 atcgggatca tctctttttc gctccccatt ttcaggacag cccccatgaa aacccgtttc
     7861 tgcatcatct ggtccctttc cctcacgttg acgatgctgg gcatgatccc ttcctgcatc
     7921 gcttttcttt ccagttcttc gctcttgttc ggatcgataa attcgtaaac caggttacct
     7981 ttggccacat tcgaaaactc aatcaggaga tctttgaaat ccttacgtgc ctgtaaaagc
     8041 tgcgggggaa cctcttcggt aaaataggcg gtcaccgtta cgggttcttc cagcgagcgg
     8101 aggatatttt tagtggcttt gctcagcgta tagcttttgt cggccgtgag gtcgagccgg
     8161 aaatacagtt ggtctgacaa aatattgatc acaatgacaa tcccggtcat cagcaggatt
     8221 ttcgttgtca ttgatttagc acctttgatt ttcatattct aagttttatc agatttcaga
     8281 attatttcga tcaggcggtt cttttcttta agatattcat ctccgtcagg attaatgaga
     8341 taaagatcac ggagaagaag aagatcaggt cgcgtgagtc gatcacgccg cgggagaccg
     8401 attcgaaatg ctgggagatg ctcaggaagc tgatgatctc gcccggtaaa ccggagaggt
     8461 tgctggccag caggtcgaag atgatgtgga aaaagatccc gatgaccagc gcgatgaggt
     8521 aggcgacgat ctggttgttg gagacggagc tggcaaagag gccgatgctg atgtatgcgc
     8581 aactgatgag catcaggccg atatatccgg tgatcacgct gccgtggtcg accgggccga
     8641 gactggctac ggtgatgtag taaggcaggg taaggcccag ggcgatcagg atcaacagga
     8701 aggtggccag gaatttgccg gcgatcacct gccagtcggt taccggccgg gtgaggagca
     8761 attcgatggt ccccgacttg ttctcttctg caaataatct catggttatt gcaggaataa
     8821 agaaaaagag cgtccagtaa gcaatgccga aaaagggctg catggttgcc tgcttgataa
     8881 agaagatgtc ggtgccgaag atccaggtaa aaaatccgct gaatcccagg aaggccacca
     8941 tcatgatgta ggccatgagg gagtcgaaaa acgaccccag ttcccgcttt gcaattgtcc
     9001 agatttggtt catatatatt ttttttatac tcaggataaa tcctgaggta agtcactaat
     9061 tcatggttaa ttccctgaag atatcttcga gttttgtttc aattgatgac attccggtga
     9121 gtgtccatcc tttgctgacg cacagtttga aaaccgctct ccgggatgaa acgccgtctt
     9181 tgctctgtat ctcatagctg gattcctcat ttttgatgaa gtcgaccagt tcgacggtgt
     9241 ccaggttttt aagggcggtg aatacagtgt tggcttcccc gtcttcgatc ctgactttga
     9301 ggatttcttt gccgtgcgcg cgtttccgga gttcttccgc ggtgccgtcg gctacgatct
     9361 tgcctttgct gatgatgagg atccggtcgc aggtggcttc gacctcggcc aggatatgcg
     9421 agctgaggat cactgtcttt tccttgccga ttttcttgat gagctcacgg atctctacga
     9481 tctggttggg atcgaggccg gcggtcggct cgtcgaggat cagcacgtcc ggatcgtgga
     9541 tcagtgcctg ggccagcccg acgcgctgct tgtagccttt tgacagttcc gatatatttt
     9601 tgtgcttctc gccttcgagc ccgcacaggc ggaccatttc caggatccgt ttccgtatcc
     9661 tcggcttttc gatgccctgg agttccgcca catatttgag gtaatcgatg accggcattt
     9721 cctggtaaag cgggttgttt tcgggaaggt agccgatatg cttttttatc tcctccgcct
     9781 ggtcttttaa agtgtattgt ccgacagata tactgccttc attgggtacc aggtaggtcg
     9841 tgatcgcctt catggtggtc gtcttaccgg cgccgttagg gccgaggaaa cctagcactt
     9901 caccggtttt tacgcggaat gtaatgttat cgacagcacg ttgcgtgccg taaatcttgg
     9961 tgagattttc tactaaaata tccatatgca cccgtaattt tggtgcaaag aaaaaaaata
    10021 gatttttcgg aaaaacagcc tgtaaagggt tttaacaaaa tttaacagac agtttatcag
    10081 ggttatgaac cactacaggg attattccac tgcagtcgtt tgaaaacctg ccctgaattc
    10141 ttcgaatttc tgcgttttgt atatgtcttt agctatgaaa tgaaggacgg cttcgaactg
    10201 cggcgctttc tggaatccgg gaagaatagt tattttgttg agtgattcat ccatgaatac
    10261 gaacgaagga tacgacatgc gcccgttaag caattcctgg gcgagctgat gcgatgagcg
    10321 gctgccggtc ggattgccgt tcacataatt ttttcctttg aaagtgactt catctttccg
    10381 ctccgcattg aatttcacgg catagaagtt atcattcata tatttgacaa tcaccggatt
    10441 gacaaaagta gaggcgtcca tctttttgca ccatccgcac cagtcggtat acatatcgat
    10501 gaagattttc ttcggtttct tctggttcat agcgaccgct tcttcgaaat ccatccattt
    10561 gatcttatcc gctgaaggtg cagcctgtga ctgcgatttg gcgggaagaa cgaataatac
    10621 ggctgcaaaa agtgcgattc cggtaaaaat gacttttttc atattttttt atttagatgc
    10681 gaaagtaaaa cgttaatttg atattatttg ttcagagtaa tttaaaattc tttatttctt
    10741 tcaatttcac gtttgatatc tttattcttc agcgtttccc gcttgtcgta gctgtgtttt
    10801 ccttttgcca gcgctatttt caatttcgcc aatccttttt cgttgacgaa taataaaaat
    10861 ggtatgatgg tcaatccttt ttcacgcgtt tttatttcca gcttcttcag ctccctcctg
    10921 ttcagtaaca atttacggtc ccttttcggg tcatggttgt aatgtgttcc gagggtatat
    10981 tctgaaatat ggaggttctt aacaaaaagc tcgccatttt cgaaagcgca gaaagaatcg
    11041 acaaaactgg ctttgccttc ccggatcgat ttgatctcgg tacccgtaag cacaatgccc
    11101 gcttcgaatt cttccagcag gaaatactcg aagtaagcct tcttgttttt tatggttatc
    11161 gttttggcgg aactgctcat tgtttctaac aaaaaagagt ttgcaaagat cgtataattt
    11221 tcgattgaat agcacgcgga ttatcacgga tgcggcggat ttacgcgaat ttaattatcc
    11281 gcgattattc gcaaaatccg cgtcatccgc gtgctattaa aatttcaatc agaactttaa
    11341 ttcaatcccc gccaggaaat gtatccccgc ctgcgggaaa tatccgtcat aaacgccata
    11401 attcccttct gaatagtacc ggtaaaccca ggcgttggac tcatattcag tattcgtaat
    11461 gttgttgatc ttcaagctga agccgagctg tttcagggat ttcaggctga tagtgtagtt
    11521 caggagaatg tcgtttgtga aatagggatc aagtttgcgc tcatcgctgg aagtattgtc
    11581 gatatattgc tttccgacat acttactgat cagggtgatt tgcatatctt taaccggttt
    11641 ccagcccagg ctgctgccgg cgataaccga aggcgaaaat gcgatatcgg ttgttctatg
    11701 ccggatgatg atctgttcgg gccattcgtc ccagttatcg acatattcgg tgaaatccac
    11761 gatcttgttc atgcttagtg ttgcatttac atcccagcgt agattctttg ccaggttcag
    11821 tgcgagtgag gtctcgatac cggcacgata gcttttatcc acattttcca taaccggatc
    11881 gcctacatca ttgatccggc cggtgagaac aagctggtcg gcataataca taaaatacag
    11941 gttggctgtc aaggcaatcg tccggctgtt gaacttatac cccgcttccg tattgtagag
    12001 atgctctgca acagggtagg ggcgggccgg gttggcatcg atcagggcac tgcggttggg
    12061 ttctcggctg ccgacggcaa atgagagata cgcttcgttg tgttcgttaa ttgtcaggta
    12121 tcccccgatt ttcggattga gaaaagtgta aatgtgatcc tggctgatgt ccctgagatc
    12181 gttgtcaatc cctgagatgg tgtaactgat ccgccggatc tggaggtcgc cgtaaaggtt
    12241 cagccgttct gccgcctgcc aggcagcttt tgcgtagata ttggcgtcga gctttcccga
    12301 ttcgctttcg taccagcgga aatccttcgg gaaaccgccc ggcgttttcg cccagatcac
    12361 ctctccgtaa tggccgccgt cgtaataatt gaccgctccg ccaatggttg ccctgacccg
    12421 gtttttatcg tcataattca atccgaaagt ataaccgtag aagtggttat cgagatagcg
    12481 gcggcggacc agatcggcag atgtgatggt gtcactacca ataatgacgt tgcctgattg
    12541 gtaatcctcg aaatcctggc cggtccggta ttcttcataa tagccgaatc cccgggtata
    12601 gtggaacgcg gtggtcaggt tccagtcgct gccgagcgtt tgcatccagt tcagatgata
    12661 atgggtctga tgataattat ctgtctgatt atcataatat tttaaattgc cattatcgtc
    12721 ggtataagcg ccaagtccat tataggtccg gttggaatcg agcaggtacc cggggacgcc
    12781 atcccaggcc tggtaggtct tctccgttcc cgaaaccatg aggagcctga caattgattt
    12841 ttttccgaaa tatcctcctg agagggcaaa ggaagacagg tcggatgagg ccctgtcgat
    12901 ataaccaccg gagttgatct ttgagaaccg gccgtcgaag gcccattttc ccttgatcag
    12961 cccggtgccg gctttgaagg tcagtttaaa ggtttcgaat gagcctgttg atgcgcttag
    13021 ttcgccgtag ggcagcggtt cggggtcgtc ggtccggatg ttgatactgg ccccgaaagc
    13081 ggccgcgccg ttagtcgagg tgccaacgcc tcgctggatc tggatgctct cggatgaaga
    13141 ggcgaagtcg ggcaggtcga cccaccagac gccgtgcgat tcggaatcgt tgagcgggat
    13201 gccgttgatg gtcacgttga tccggttaag gtccgttccc cggatgcgca tattgctgta
    13261 tccgatcccg ttacccgcat cggaagtggt cacgaccgac gggctggtct ggagcagcat
    13321 tggaaggtcc tgtcccaggt tgagggcacc gatctctttg gaagtgatgg tcgaaaaggt
    13381 agcaggggag ttattggcag cgcgggttgc ggtgacgatc acctcagaac ccatgatggc
    13441 tgaaggcctc aggctgattt caagtgtagt gtctttattt attttgatgt aagatgatga
    13501 cttttcatag ccgacgtacg atacgataat acggtatcct ccctgtttca gtcctgttat
    13561 ccggaactgc ccggaagggg aggagatggc ggcgataaag gtattttcga caaggatgtg
    13621 cgccccggca agcggttcgc gggtttccat atcactgaca atacctttta aatcaacctg
    13681 cccaaatgac aatagtgaaa ttaacacaat ccaaattgta aaaattaaac ccgttttcat
    13741 tttagcttta tttaactaat gactaatgac caacgactaa cgacaataga tccttcgtcc
    13801 gcctttgtcg gactcaggac aagctgtcca gctgtccaac cgtccaactg tccagctgtc
    13861 caactgaaac cgggtaccaa aatcaaagag aggatatcac attcctacgc cggcattacc
    13921 cggatcaggt tcaatgggta tgatctcagc ctgaaattta aggcacccct gaatcagtgc
    13981 aaagttaata gttaaaagtt caaagtgggc agttagtgtt aagtttttag ggttaagggt
    14041 taagcggggg gatttttttg tcatctcacc agcatcaccg tcccggcccg ttcctggctt
    14101 ccgggaacac catctattga aaatacgatc ttataaacat acactccgcc ggggcattcc
    14161 ttcccgttct tcttcccgtc ccatcccaat gagatgccat ctccaaaaaa cacttcccca
    14221 ccccaccgat cgaaaattct taatgttgaa tgttgaatgt tgaatgttga attcgtgagc
    14281 ttgatttgaa aactgtcgtt gattccatct ccgtcgggag aaaaggcgtt gggaatgaat
    14341 attttgaaat cggggatttc ttcgaaggac agtttaatgt agatggagtc tcgtccgatg
    14401 caaccgattt gagactccat ctcaaccagg tattttcctt cagcctggat cacaatgctt
    14461 tgcgtcgagt atgcggtgtt ccatttatac gacgccatcc cttcgcctgc atccagcacc
    14521 tcgccggcat gcatttccag tgtatcggtt ccatggaaag cagcaaccgg attttcgctg
    14581 acgatcagct caatgctttt ccggtcggtg cagccgacgc ggtcggtggc gaggagggtg
    14641 tagaggccgg catcggcggg ggtgacggag aagaaaaagg ggtcggtgct ggtggaatcg
    14701 cccgttggat aaatccaacg gaaagtcagc gggttctggt tgccaaccgc gatgctcatc
    14761 aggctaacga attgcccggt gcaaacggtt ttgctttgat ccaggataat ctgcggtggc
    14821 tgatagatgg ttacctgccc tgtgctgaac tcagctggga tcggattgcc gctgctgttg
    14881 gtgaaatagc tctccgcttc cccggtgtac cacgaaagct ctccctgccc gggatttttg
    14941 gtggtgaaaa ccaggtcggc tactttttcc ggctgactaa aagtcagcgg cgactcacca
    15001 ttccatgtca gatggatatc tcctgcaatc gcatcaaccc aaccggtaag gctatccaac
    15061 aattgaacat taacattagc aaagccttcg cattccagat gaccggcatt atatcccagc
    15121 ttcaggtgaa aatcggcaac agcattgaaa ttatcgacat tcaccggcac cattgccgta
    15181 ttcccaagac aatgtcctcc ttcaccggtc acagcatgca accatgtcag aataatattc
    15241 tgaatctcaa cagtaaaggt ggtgtcacag ccattttcat cccaaacttg taaattgtaa
    15301 atcctggatc ctagatcgtt aaaggttcca ttgttcaatt gataagtcgc tccgctgtct
    15361 attgaataga aaataaccgg agtagatcct gttgccacaa tttcaatcgc gccatttccc
    15421 aggaaatcgg tttcatccgt aacaatcaca tcaacgacct gaggaccagg gatatcatcc
    15481 aataaaaccg gattatcatt ataaaatcca aaacaaccta ctccatccgt aacccgcacc
    15541 acgtaacccg caccgatcag cccggagaac acactatccg tttggtatga atcgccgtca
    15601 tcgattgaat actgcaatgc agaaccagcg ggagaaagcg catgaataat gatctgacca
    15661 tcaggccttc cgcagtgcgg gcgggtttgt tcaacgttca gaacctgcag gttcccggca
    15721 tcttcgatgg tgtaaacatt ggaaactgtc tcgcaaccgt tagcgtcagt aattgtgaga
    15781 taatattgcc ctgccgggag tccggacacg tcaatatccg ttccaacctg gacctcggag
    15841 aggtccttcc atctgtacgc gaaaggcgaa tagccaagag caatgagccc tgtaatcgat
    15901 ccgcttgctc cgttgcaggt ggtttgtgtg attatcaggt tggtttcatc gataattgtt
    15961 ggagcgtgaa agccgacatg gatcgtatca tatcccatgc atccggaatt gttgaaacgg
    16021 ccacgtaccc agtaggttcc tgaatcagaa acagttatcg atgaaacgcc gaactgtccg
    16081 gtggaccagg agaagaaatc ggcttcgcag tttgcgctga gcaccagcaa agcgtcttta
    16141 cagatcaacg tatccgggcc gagatcgggt tgcggtgacg gtaagatctc tatctccctg
    16201 gaagtgtgct catatctccc ggaagggtac cacacatcaa ccttcacctc atgaacaccg
    16261 ggatttttaa aagtataggt cggtgaaagc tgccacgaat tgcttcccgg cccatcatca
    16321 aaattccatt ggatggtttg aggtgtagga atgaagttgg gcttgaagtg gatcgggtag
    16381 ccctggcacg tttcgccggt ccattcgaag cggaggaggt agtcgacgag gatgttgggg
    16441 aaaccccacc ctacttgatt tggaaataag ttaagtacat tggtaacgag ggaacatccg
    16501 tctcctctga tccaaggatt ttcaatcacg ctgataaaat atctcttatt aggatccaag
    16561 acctggagag gtgtgcaata aatccttcca tctcttgcga ggtgtaaatc aaacgtatgg
    16621 taggtattaa ttttcaatgc ggaactttta aacatagctg aatccgtaac atattgcata
    16681 tcatattggt acaattcact tgtgtcaact ccattaaaat ttacataaag atattttgaa
    16741 tcaggagaaa attcaacacc agaacaacta ggagcgacaa tacttggact tgtaattaaa
    16801 aacaagtgaa cagcatttcc atttgtagca ttaaagctgc atacttcaaa tccatgacca
    16861 ccatatgtta aagtcaggaa atgtttatca gaggagattt ttatataccc ccacatactt
    16921 tgccaggtca tttcgatatg tggcattgct gaaataaccg ggtcggaatg aaatccattt
    16981 tcatcgataa ggaatgctgc aatggcctcc tcttcattct ttctgactat tacccaaaca
    17041 tcatccgatt tatccttctt tgtgattgca atgcgatcac tcacatccca acattctgga
    17101 actggaacat ttagttctgt taccacaccg ccaagcccca ggctaatatc tacgatggaa
    17161 taatagaatc cggcataggg taaattagga tcggcagctg taaatacata atatagagaa
    17221 tcctgtccag gccatttgac cgtggccaaa cctgatattc ctgtattaac cagaccgtca
    17281 ctattttgca tgatgccata aattgaatat atatattttg tatcaacaca aaacaagaaa
    17341 ctcccggatg tatcactgat agttgaactt gctgaaccat gtactgcctg tccatcatac
    17401 agaacttcag gaattcccat attgaaatcc aagccgcaac catgtgtgaa gtataatata
    17461 ttagcttcat tctgggcgat agataattta taaatcacaa ataaaaatag tacagtaaat
    17521 agctttctca tgcattttga aaatttaaaa aatctgccgg cgtaacgaaa cgccgggcag
    17581 actttcatta taatttatca ccataaaaca gtaaccggca catttggaag agtcatcaat
    17641 tcgttgcaag tataataatg aactccatta cctgtgcatt ttgctacatt tccgccaggt
    17701 ccattgcagt attttgttgc tatgccaacc aaggcataac atttctcatt tgatgggtca
    17761 tcacatgaga aatcacaatc aaagtatatt tcctccgttg aacaattcac ccattgcgga
    17821 ggagcagtgc agcaaagttc aggtggttcg acacatatat tatacagttg atattcaact
    17881 ttatatgtgc atatttcatg agaagtacac gtatcggggc attgaccatc caatgttact
    17941 ttaatggtaa tttgggcatt taggctttgc gatgcgataa aaaaaataac cgaaatgaca
    18001 gcaataatat ggattaatct tttcatttta aatggttttt aaagttaata aattctaatg
    18061 gatgctgttt aaaagacggg caaataattt tcattcaggg ggacaaaccc cttatggagg
    18121 atgggaatga gagaaaatga tattcgattg tcgattgtcg atttaatttt agagtttgag
    18181 tattggattt tggaattgat tggaatttgg tccgccagtt ggcgaatgaa atttggaatt
    18241 tcatgattaa gatcattttg aaatgattgg cataaaagta agatgtttta ggaagaaatg
    18301 caagggattt cagttgggca gcatatccct ccttcatcgg gatgacggga gggaaccttg
    18361 atgaacctga ctttccgaat aaatatctaa ctttgaactt tgaactttga actttgaact
    18421 tgtttattat caacgggcct aacctcaacc tgcttggcaa gcgcgagccg gaaatctatg
    18481 gcaaccagtc atttgacgag tttctggata tcttaagggc cgaatttccg ggtcattcca
    18541 tcggctatta ccagtcgaat acggaggggg agatcattaa cgcgatacag gatgcgggat
    18601 ttactacgga tggcattatc atcaacccgg gaggttacag ccatacctcg gtagcgattg
    18661 ccgatgcaat gaaagcggtt cctgccaggg tgatcgaggt tcacatctcg aatgtttttc
    18721 agcgggaaga ttttcgtaag gtcatggtaa ccgctaccgg cgcggatgcg cttatttgcg
    18781 gttttggcct ggaaagttac cggatagcta tccggaatct taccggggat tctttgtaaa
    18841 ccgctcagga tctagctgtc cgaatgattt cttccttctc aggtaatgct cgaccacgat
    18901 atttccccag taaatttctg aaacgcgatg gtgcgggatc ttttcccttt tctttctgag
    18961 caaatataga tggctccaga agtaaatatg ggctttgaac acggcgaaca tatctttaaa
    19021 accgccgtcg accaggaatt tcattgcagc tactccatcg aggataaaac gggccagcat
    19081 tacacttaac agtctgcccg gctccaggtt tttgtaaagc ataatgatgt tgttgcgaat
    19141 gttgagatag gtcttgcgcg agtttttctt cggaagcgtt cctcctccga tgtgaaatat
    19201 agttgattcc gggcaataca tgattttata cccctggttt ttcgctctcc agcaaagatc
    19261 gatctcctcc atgtgggcaa aaaaatcgtc atcaaagcca cccagttgcg tgaatacaac
    19321 cgacctgacg aacaggcagg cccctgtggc ccagaacacc tcccgcgcct ggtcatactg
    19381 cccttcatca ttttcaatat gctggaacaa gcgcccctgg cagaacgggt acccgtattt
    19441 atcgatgaaa cctcccgcgg cgccggcgta ttcgaactgc tccggctctt tgtacgacct
    19501 gatcttaggc tggcaggcgg caatggacgc atcggactcc atcagccgga tgaccggtgt
    19561 gatccagttt tccgtcactt caatatcgct gttcagcaga ataaaatact cagcgtcaac
    19621 ctgttttagc gcccggttgt accctccggc gaaaccgtaa tttttatcca gccggatgat
    19681 gcgtatgccg ggatgggcag cctcaaggaa ggccagagaa tcgtcggcag aatcgttgtc
    19741 ggcgacaatg atttcggctt caccggcgct gtgggcaatg actccgggaa ggaatttctc
    19801 cagaaattcc cttccattcc agttaagtat gacgacagcc gttttcaaac aggtgatttc
    19861 tgcttcaaat gtaaatcaaa attatcagaa atagtaatac cgggtcaatt accgggtgtg
    19921 cgggtcagct tccagcggcg gtgcgaccac agccagttgt cgggacgccg gcggatagct
    19981 tcttcgagca accggatata agcttccgta atttccttct ctgccgtttt ttccggctcc
    20041 cgggtgatct gcctgatctc tccgttgtat tttccattgc cggtccgttg tatgtcgatg
    20101 aaaaagacgg caaggtgaag ggcttttgcc agtttctcca ccccgatgta aaaaggggta
    20161 tcctggttta aaaatcgagt ccagtagttg gcctcttctt tcaccggcgt ctgatcggcg
    20221 gcgaacacat taaacgtcac catttcctgt ttatgccgga tcagggtgcg gtaggtacgg
    20281 tgaaagggga tcgtgctgtc ggggttgatc cggtgccgga gagattccat ataccggtta
    20341 aaatgtttgt cggagagggg tttgacaatt gcgaaccccc tggcgggcag cacctgcccc
    20401 agcgccgtcc ccatccattc ccagttaccg cagtgcccga tagttatgat gacgctctgg
    20461 cccttacgca gcgtctcagc cagtggctcc agtcccgtaa aagtaaaccg ttccagcaat
    20521 ttttcaggcc ggatagaatg cagcttgatc acttcaaaca tgatctgcga caggttttta
    20581 taatactggc caaggatttt tcgtatttct ccgggttttt tttcaggaaa cgcgttacgg
    20641 aggtttttaa ggatgatatc tctccggtag cggatgatat aatgcaggaa aaaattaaag
    20701 atacccgcga cgaagaatag taccgggaat gggagccgtg agaccaggtt ggccaggagt
    20761 ttgaaagtat ggtacaggaa tgccatttgc cctaagattc aggatggcaa agatactatc
    20821 aaacgttcaa aaaattgtaa tgaccgttta acgcgggtta cggtaataat catccgcttt
    20881 tccgccataa attccggggg cctggttggc atccacgtta taggggtcat accagcggtt
    20941 gttcaggaaa acctgccact tgtaattctg gatctcgccg atggcatcgg agtgtaaaag
    21001 ctggaagttg ttggtaatga tgttctgggt atagaagaca aactttttat tccgctggtt
    21061 gccgccaagt tcataataat gccagatttc ataagggtag ctgctcggct cgttatagtt
    21121 ttcgctgata atattcggcg ggccgtattt caggtaaacc ctgccgcggt cggtatcgta
    21181 tcctttgctg atctgtgttt tgtaggcctt attcaccacc tgaacctggc cataataatc
    21241 tttccaggca gtttcgggat tgatcatatc cctcgacgac cagaaattgt agaagaactg
    21301 ctgtttcgtc ttcagatcgg ccagtttggc ctgcttgtaa atgaaatttt tctccagttc
    21361 cgttgcgatg ggatcgaggc agttgatgaa ttcctgcagc gtgtcggcat tcgtaatgcg
    21421 gctggcgaag gtggcgttga cgtcgatcgt ggccaggtcg tcgagactaa actggatatt
    21481 gggattgctc cgctggaaaa acatcttgtt ttcagcaata acctcattct gacggttctt
    21541 gacctggaaa accagcagga aattgccgct gggcagccgg ctgatatcga actcgccgaa
    21601 aaccgggact accgggccgg catcgaattt ccggtaggtt acgaattcct tcagggtgtt
    21661 ttccgtttca aatgaacgga tcatggtgac attcaggaac ttttcacccg ggccgagcgc
    21721 tttgtccgag ttataaattt cagtataata gatcagctta ttggttgatt cgggataaaa
    21781 attgtacacc agcggaacga ggtcatatcc gcctttggtg aggatatttt gttgggatga
    21841 gggggagaaa gatttgacaa actgtactcc cgaaaagcag gctgctttat catcaaaatt
    21901 gatggtgacc ggttgggtag cggaaaaagg ttttttaccg gtattgaggt ctgcgatcat
    21961 gatttccatt tcgtattccc cttcggggat gaaataccgc tgctggtcaa tgaagctgaa
    22021 atcgatagcc accgtatcgc tgactaccgg gctgaacaac tggtacttgt cgaattcctt
    22081 gatctcttcg ccctggcgga agatgaaagt gacttcgatc tttccctgga actgccccga
    22141 ttcattgggc atgtaaaaca ccgatttccc ttcgaccgaa aggtacgttt cgatgaaggg
    22201 ccctttgccg gggacattaa aaacagtgta gttgagatag gcctgcagcg ttttcgactg
    22261 cattacggcg ggggagagga gggtgtttat aaggatggca acaaataaaa ttcttttcat
    22321 tggcaaaacg atttaagaaa gtttcaattc ccgttaaaac aaagataaat ttaaaatatt
    22381 taaaaaacta ttttgatcag tttaaggccg ggataagtca gaatgtggca aattttggcg
    22441 gaaggtagtt ccgggaagat aaaagtttgt atttttgcgc cctcaacgga gaaatggcag
    22501 agcggtcgaa tgcggcggtc ttgaaaaccg ttgtcccgat acctcgggac cgggggttcg
    22561 aatccctctt tctccgctcc ggagagatgg cagagcggtc gaatgcggcg gtctcgaaaa
    22621 ccgttgtccc gatatctcgg gaccgggggt tcgaatcccc ctctctccgc agaaactttt
    22681 cttaagatca atggttccag cgttccaggt tacaacctta tgacaacctt atgacaacct
    22741 tatgacaacc ttatgacaac tttatgacgc gagcgaagcg agctaatgac ctattcccgc
    22801 gagcgcagcg agctaatgac tattttcaac cttcaccacc ttcaccgttt ccgtctcttt
    22861 cccgtcgatg gtcaggcgga caaagtagat tccggcgggc agggcggttc ctccagggcc
    22921 ggctccatcc cagtagattt cccctgtctc ttcggtccct cctttaaaat tgaccagtgt
    22981 cttaaccaac attccgctgc tgttgaaaat actgatattc aacctcccgg ctgatttcaa
    23041 ctcgtaactt atataagtcc cgaaaataaa aggattcgga ttgacgttta ataatttgga
    23101 acccggaacc tggaacctgg aaccgggtgc cgaaggcaca ttgacccagg ggccatattc
    23161 gtatgctccc atatcaacgg attctcc
//
LOCUS       DAOPNH010000011        22290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2175_length_22290_cov_6.520711, whole genome shotgun sequence.
ACCESSION   DAOPNH010000011 DAOPNH010000000
VERSION     DAOPNH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_22290_cov_6.520711"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="PKZ74_01410"
     CDS             <1..1303
                     /locus_tag="PKZ74_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPM91434.1"
                     /translation="ALIIANDATIKGGTYVKETIKKHVRAQQIAMDNHLPCVYLVDSG
                     GIFLPEQSKVFADRFHFGRFFYNQARLSAMGVPQIACVMGFCTAGGAYVPAMSDETII
                     VKEQGTIYIGGPPLVKAATGEVVSPEDLGGAYVHTSISGVADYLAENDRHAIQIIRNI
                     FQTLERKEKQILATSAIEEPYYDPEELYGIAPIDFKKPVNAREIIARMVDGSRFQEFK
                     TNYAPTLITGFANIMGYPVGILASNGVLFSESSLKGAHFIELCTSRKVPLLFLQNITG
                     FIVGKQYEHGGIAKDGAKFVHAVANADVPKFTVVFGGSFGAGNYAMAGRGYDPRLLFM
                     WPNSRISVMGGEQASHVLIQVKEEQLKAQGKEMDPVEREKLRAEILKKYDEEGSPYFS
                     TSRLWDDGIIDPVDTRKYIAMGIAASLNCKFPDTKYGVFRM"
     gene            1396..2220
                     /locus_tag="PKZ74_01415"
     CDS             1396..2220
                     /locus_tag="PKZ74_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPM91435.1"
                     /translation="MNTYTTIEFQKQGDLATVWLNRPDIHNAFNEVMIREVIDCIEAV
                     NTMEDIRVVILRGKGRSFCAGADLNWMRDVAKYSYEQNFQESLNLSKCFYTIYTCRKP
                     TIAVVHGAAIGGANGLLASCDFAYAEDNTTFSLSEVKIGVVPACISPYVIKRVGEYGS
                     RELMLTGRRIKGKEAEQFRLVNKSLPEAELDAYVTSVIDLLRTSGPKAMTQCKTLIYN
                     ICNIESLDQARESTARMIAEIRASEEGQEGMAAFLEKRKPNWTDPSPAFGGPRTSD"
     gene            2240..4240
                     /locus_tag="PKZ74_01420"
     CDS             2240..4240
                     /locus_tag="PKZ74_01420"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPM91436.1"
                     /translation="MMLFSKILIANRGEIAVRVIRAVQEMGMRAVAIYSEADAAALHV
                     RMADEAYSLEGNDLSQTYLNIPKIVGIAKACGAGAIHPGYGFLSENAAFVKACDEAGI
                     VFIGPNARAMKLMGNKIKSREALKDSGIPMTAGITGDVETLRKRAAEIPLPIMIKAAA
                     GGGGKGMRIVHDTSNLAELLESTSREALSYFGDGTIFIEKYIEDPRHIEIQVLGDNYG
                     NVIHLFERECSIQRRHQKIIEESPSPTLTPEIRANMGETAVRIAKTIGYNNAGTVEFL
                     VDKNLKFYFLEMNTRVQVEHPVTEMVTGIDIVKEQIKIAAGKKLEIGQESVRQNGHAI
                     ECRIYAEDPANNFLPSPGKMSYYLEPEGEGIRLDSGILEATMIHSFYDPLICKLITWD
                     EDRESAREKMIGALDEFIIHGIRTNIGYLRHLLKNQDYISNNISTKYCDEFTGNLNGE
                     LLSEKDRIPLFIPGLAYYLYDFNCKPVTGDLQGHDIWNHISYWRDLMDFEGEADGKPF
                     RFTVDKIKDGRYLINAAGEAHEAQIRFCGEGYLEMVLDGRQHEFYISTDDQGTGWVSF
                     DGFIFHVNRNDILAGNQAILGSLDHSGGKQDGKIISPMPGKVIKINVGEGDEVKKGQT
                     LLIVEAMKMENYITAPNDGIIEDIKVKVGDMVNSNTKLIEIS"
     gene            4440..5585
                     /locus_tag="PKZ74_01425"
     CDS             4440..5585
                     /locus_tag="PKZ74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPM91437.1"
                     /translation="MNYDLTEEQNMIRETVREFAEREIKPVAKELDEKSEFSVDLTRK
                     MGELGLFGMYLPEKYGGQGLDYLSYIIAVEEIARIDSSQAATLAAHNSLGIGPLYYYG
                     TEEQKMKYLPQLCTGFALWGFGLTEPDAGSDSRGTKTKAYLENGEWVINGSKIFITNG
                     SVDISVGSTVQAVTSDTDGRKLYTCIIVEKGTPGFTRRGMHGKLLWRASDTAELFFDD
                     CRVPEANLLGRIGEGSHIMLSTLDSGRLSIAAMGLGLAQGAYELAMQYAKERKQFGQP
                     IIKFQVTAFKLADMATKIELARNLLYKACWLKDNGKPFGKEAAMSKLYCSEIAREVAD
                     EAVQIHGGYGLMKDYDVERHYRDQRLLQIGEGTSEIQRLVISRYIGC"
     gene            complement(5565..6581)
                     /locus_tag="PKZ74_01430"
     CDS             complement(5565..6581)
                     /locus_tag="PKZ74_01430"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HPM91438.1"
                     /translation="MTKLFYLLPGLPALFLIIAPGFGCNKDDDNMPDATPVDYSLVEH
                     WLSVPAPEKEVDIFYLYPTSWQKLNPDDPNICDIDNPMMLSGSASAYHRQATAFATTG
                     NIFAPYYRQADAQYTLSLPEAQRDSVIGGIPLLDATAAFDYYINHFNDGRPFILAGHS
                     QGSNILLFLLSGYLKQHPEVYSRMIAAYVIGYPVTQAYLTANPHLKFAERADDTGVII
                     SYNTQSPAVSPGQNPVVSDIVGLVINPISWTRDETPATTAEGLGSYMPDQEGVFAQVP
                     QYADARIDTGQGVLICSTAVDSTLYLGFGPGVYHSYDYPFYYFNLRANAGLRAGKFLA
                     ADIP"
     gene            complement(6672..7037)
                     /locus_tag="PKZ74_01435"
     CDS             complement(6672..7037)
                     /locus_tag="PKZ74_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91439.1"
                     /translation="MPIKYVFSVEDELLKVTAHGKDDSFEEVMNYSRTIIEASRQHGC
                     TKILCDERHLQHAITATDTYLLAENASQHASYFSKVAIICHKRSLKEGKFYETVASNR
                     GLKILVTTDPTAALGWLDK"
     gene            7340..8596
                     /locus_tag="PKZ74_01440"
     CDS             7340..8596
                     /locus_tag="PKZ74_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPM91440.1"
                     /translation="MKKSFIAVFILAGAIISGHSQSFTAYMLVAPEAYLEDVHKISIL
                     DFDGRMGTAMTDYLAAELMQEFRGTGGKSGGNYFSAGKSINNFQKGARTNVFSLVERS
                     ELDKVLREQNISNSGLIDDSQAAQIGKLLGIDAMITGSVAYSHQDEQKTLTYTDLNGN
                     KKYSYCTTRTVTAEGHLKIINVNTGQIIGTTVKTTSYSDKKCDDKRSTLFSVEELAKV
                     CYDGLATKLANYFTPVFLPMEYEFEKIKDKEYKEKGKLAMDYLESGDLNNAFSIYKSI
                     YDIDPYNSAASSNIARLYRIAGNYEKAREYYALAAEVDEVTYAPLVQAAIREKEMLSL
                     FEGYGINIEQTDFIQKSDALAERVTTRGNKSDKYDVRVNPDQTSDVVAKVPGSTEFTV
                     IEKQGEYYLIKLLGGKQGYIHDDYVK"
     gene            8666..9706
                     /locus_tag="PKZ74_01445"
     CDS             8666..9706
                     /locus_tag="PKZ74_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91441.1"
                     /translation="MKYLNPILFFVLFPFLATVAQPEIPAWCDKSFRESHYPADQYLT
                     GYMEGGKIVNETLDQARERMKKLAQSELIEGVMVQIQSYTESKVQDITRQSTSSVVQF
                     YEASVKTESNMELTGLTNEVFYNPETATVYAFSYANRYEIIGYYKANIGFYQQQIIQV
                     VELSDRLYTDGEKVKARSELEKAIPLFARIDYSYGVLLALKGNIDSLQYANSMQMKST
                     AIRKLADLEKGVIVFVQSSEDIFGSPSEILANALKRDLKGCSFTADESASDWKVTIDA
                     KAREYSYTNGFYFSYADVVLSLMKTRTGQLLYQEEFSQKGGGSDYRKAARKAMEEAAT
                     AVSGKIVAEINK"
     gene            9721..10347
                     /locus_tag="PKZ74_01450"
     CDS             9721..10347
                     /locus_tag="PKZ74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM91442.1"
                     /translation="MNPLKKLAGQTLVYGMGTIVPRLLNYFLLTPFYTRIFITGEYGV
                     ITELYAYMAFLLVLLTYGMETAFFRFAGKEPDPKKVFSTSLVSLFVTSVLFVFLVVIF
                     SQPVATLIEYGNNPEYITMFSVIVALDAFTAIPFAYLRHRNKALRFSIIKIVNVLVNI
                     GLNFFFLWLAPKMLSANPGSWVRLVYDPAIGVGYAFIANLASSGLTLL"
     gene            10348..11208
                     /locus_tag="PKZ74_01455"
     CDS             10348..11208
                     /locus_tag="PKZ74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM91443.1"
                     /translation="MLPDIFSVRPAVDKDLLKRMLTYAFPLLIVGLAGMVNEVADKII
                     FKFLLIVPPGEADPKAYAMSELGVYGAAYKLSVLMTLFIQMFRYAAEPFFFAQKKEEN
                     AKKIYADVMKYFVIFCLIIFLGVTLFADIVKYFIGPDYREGLFILPIVLMANLLLGIT
                     FNLSIWYKLNDMTRFGAYIGLSGAAMTILMNVLLVPRLSYLGAAIGHLSAYSLMVILS
                     YYWGQKYYRIGYPMKRIGFYTILTLALFLPGYYLPVGNQALSLAINAVLFCIFLAVVY
                     FKERREIRGL"
     gene            complement(11255..11992)
                     /locus_tag="PKZ74_01460"
     CDS             complement(11255..11992)
                     /locus_tag="PKZ74_01460"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM91444.1"
                     /translation="MIKRLLIISVAALMLIAVSCNKDKTNIAPTIRFTQPDSLLVVTH
                     DTSLVFIAEPEDVDGNITKVEFYINEATVSTDLESPYVYEWLDAKLENAGNYLFRAIA
                     YDNDGATGEDEIQIEIFDYREKFLGDFRFTVINESWSMGQPNTYDTSVYAGIIRRFDP
                     ADSIDDLYYIQDDINEVPDTKVTIIFGPSAKITSVLDTAGALLPKGGYHYWHSGGFTT
                     TDSIFFSVGGLGGLGAGNNYYVTGVKQ"
     gene            12453..14144
                     /locus_tag="PKZ74_01465"
     CDS             12453..14144
                     /locus_tag="PKZ74_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91445.1"
                     /translation="MKSVLRLLIFLTVLQSNSPLIHAQGQFSKNRETYYEATKQKILG
                     NLDKAIELFKKCIELDSKDAAAMYELASIYTDLGKPAEALPFAEKAVAADPSNSWYKI
                     LLVRILQAQGKYSDAAKTIDQLLVSDPENIDYYQDQALNYLYDGDYKNAVKSYDMLEQ
                     KIGINEDLSVQKEKIYLMIGKTDKAIEEIRKLGQAFPDEPRYMEMLAELYMNTGMYDQ
                     ALELYNRILEADPDNPYINISLSDYYRKTGNRDKAYEYLKAGFANPALDIDSKVSILL
                     AYYSVTDIYSDYKEEAFELAEILIRAHPNDPKAHSIYADFLVQDKRYAEARDTFRKVI
                     SLDSTKYLIWEQLLFVESELNDNQAMAAESKRALDLFPDQPMLYMFAGAAHFMLKDYE
                     AAVKYFRAGVNFVVGNDRMLEQFYSYLGDSYFQLKDHEKSDEAYEKVLKINPANTLVL
                     NNYAYYLSLRGKDLEKAEQMAERAVEMEPDNSSYLDTYGWVLFKLGRYEEAKPWIERA
                     MEKGENSAVVLEHYGDVLWKLNDKKEAVKYWEKALDAGTGSEFLEMKVRDKEYHD"
     gene            14137..14955
                     /locus_tag="PKZ74_01470"
     CDS             14137..14955
                     /locus_tag="PKZ74_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPM91446.1"
                     /translation="MIKIFKRIGMMGLVILALSSCRSTRPLIKEPLKEQGPEYLYSRL
                     KQSEFRFDRLSMKFTASYSDKKSSADLNGQIRIRKDSLIWITASPAMGLEMFRMVITT
                     DSVKYLNRMNKDYFAGDYALVSRFLQIDVDFDMLQSILLGNDFQFFETNNFRASIDNL
                     NYKLSTTGRRKIRKEAESAKNDPVILLQNIWLDPSSYKIKKIDLKEYMKDNRKLTATY
                     GDFQALNDQIYPSDIRFEIQAEEKSKVRVKYDKVATGEQMTFPFSIPPNYQRIR"
     gene            15002..16222
                     /locus_tag="PKZ74_01475"
     CDS             15002..16222
                     /locus_tag="PKZ74_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPM91447.1"
                     /translation="MNRFTFFFILLIMPFTVAFAQKDRTKLEQDKKKIEEEIEYTNKL
                     LDQTRQTRQNSLNEVVILNKKISQREQLINTISSEIGLVDGQMALTLDTIKLLENDLK
                     SLKDEYAQMIYYAYKNQNLYDRMLFIFSAKDFNQAYQRLKYFEFYNQYRRQQALLIQV
                     KQKRLEIKMEELAAIRTQKNNLLTGEERQRQQLTVEREEKNKSVKALSKKEKELQKTL
                     KEKEAAAKKLQQAIQEIIAEEIRLANERASKYGAPLPKTGVYALTPEEKIISDNFQSN
                     KGKLPWPVEQGIVSGTFGEHAHPVLKNVTTRNNGIDLLTQAGTEARAIFSGVVTRVMS
                     VPNNNNVVIIRHGEFLSVYSNLDQVYVKIGEKVTTKQKIGTVFTNKEDSKTELHFEVW
                     QSKTLQNPVEWLAK"
     gene            complement(16236..18227)
                     /locus_tag="PKZ74_01480"
     CDS             complement(16236..18227)
                     /locus_tag="PKZ74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM91448.1"
                     /translation="MKKITSFFIFLIIIPFLISNTPANKHEKPYAEGEIMIKLYSDLP
                     QNQQQMLQDVLGDFQGAGLGVIRRLSDRLNIFLLSYDPSVISDDRMLQEIKSHPFVEL
                     AQFNHFIELREFIPNDPSFSQQWNMNNTGQTGGQPDADIDGPEAWGISTSGVTATGDT
                     IIIAVVDDGFDLEHEDIRFWKNYQEIPGNGIDDDSNGYIDDYHGWNSWNNSGNLVEKD
                     HGTHVTGIAAAKGNNGLGVTGVNMNVKVMPVVGSATTESIVVGGYSYVYEMRWLYNKT
                     DGAKGAFIVSSNSSFGTDGDIEDFPIWSAMYDSMGMQGILNAAATVNANLNIDVVGDI
                     PTAFPNEALITVTNTTSDDELSPYAGYGLTTIDIGAPGTQVYSTRYNNSYGLKTGTSM
                     SSPHVAGAIAYLFSVADESFMESYHADPAGMALVLKQYILNGADPIPTLANKSVSGGR
                     LNIYNAAQLLLNPAISFDPMSLLQMMQPNEQESVILSFTNNSASSVSYEIALAGTFPW
                     IALTGATSGTLSGFGTGSIQVDFNTTGMPYDTLQTYIAFNYGNDGLFNVPVHLLIGPF
                     IGLDDLSGNPDRLSKSLAIWPNPARDVIRFKVAGVSEGSVYSIDMVSADGRIHHTLNS
                     GSNQNELILDARSFKPGMYLAILKENDQVIDSQKILISR"
     gene            complement(18494..20302)
                     /locus_tag="PKZ74_01485"
     CDS             complement(18494..20302)
                     /locus_tag="PKZ74_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM91449.1"
                     /translation="MKKIPMTSYLVILLCGTVLQAFTQVNKSLAPTLLTYTESSSGLA
                     SPEWEGGRSELEFADINGDGHPDIITIGDHGNPGIQSGEQGIMVYFGDGQGNWEVQMG
                     GDLGYGGIAAGDVNNDGLMDIGYGMHHNYSSTDLGDQLIEVALGNGTGTGWIPWDDGL
                     ATNGEDWGMFGTDFADVDNDGDLDIGSISFGCCSGIHVYLNNLDGTWTQSFGFNTGNS
                     SMIFQFGDINNDGNMDFIVDHESGTAYFGDGTGGFSINDLNLPSLDNIGRSGPSLGDA
                     DNDGGMDLAFTNYDGGLHVYTFMEDIGEWADFSGNLPSSGDFEMTQLADMNTDGFIDL
                     AAFGTGTFRVFLGNGQGNWTADATFSVGDPGSCQAFRVGGDADHNGKPDVVLLEETNL
                     SWFTYQNFLHFFKENSTPLALDIRPVFPRGHELLKPGSVRNIGWISAVYGNQESFVQL
                     EYSVTGPEGPWIPIAAELPDNGSYQWIVPQENSDNCYIRYSMYAGQSNAQGMTTAPFT
                     ITDGSIGISDETDKISGWRIYPNPATSILAIDLHRPAARLSVITPFGQTVMQIEDIQS
                     FPFTLDISALSPGLYFLRMEDENGLSGTLKFIKETV"
     gene            complement(20306..>22290)
                     /locus_tag="PKZ74_01490"
     CDS             complement(20306..>22290)
                     /locus_tag="PKZ74_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91450.1"
                     /translation="DGGWVETSDTAAIFRWKLSAEGNLGTTDINFTVKLEEDGTIGFI
                     YGPSTLGGFGWLAGLSAGNKTDYLVSPYSGQPQIPPGTSVTFRYQSLPGTFQLSENGL
                     LSGTPHSDDRIHDVAVRVTDRSGISVARDFQLTSGPYIYLSVMPGNDDQVFYGDTVSL
                     QLTLVNGGTEILENPFISLSTSDPFIIFLDSQCQTGTIPAGESVSVTDAFSFVVSTFI
                     PDHSDIQFETVLDASGRNWVKDLVLQSNAPGLFLERNEIGDEDNRLSPGETAPLLATL
                     RNSGHAAIDQVMSELVSLSPEVVVSGNTVQDFGSIGKGASVTRSFTLTADDSTPSGTP
                     VTLVLTTTSASGIGQQDTIEMKVGKTPVMVIDMAVDKNSGPVIFSLLNELNVLSEYDY
                     SITPKIFDYESLFICLGYYYQNHLLTLGEGTLIANYLDAGGKVYLESRTTWKDDPGTP
                     AQPKFNIQWAGNITPFDTITGIDTAFAAGLTFTNEAIHPFSFYYLDAIPPAFPILEDN
                     STRLPCAVAYDAGNYKTIGALFEFGTQPGIPSGASTELLTRYLEFFGINVSPIGTDEI
                     NGQKEVMNLHIYPVPATNLININIQDPVTRNPSPVIPTSLFVINLYGQTVMQIEDIQS
                     FPYTLDISALSPGLYFLRMEDEKGHSDTSKFIKAQN"
BASE COUNT         5798 a         5373 c         5916 g         5203 t
ORIGIN      
        1 agcgctgatc atcgccaacg atgcgacgat caaaggcggc acctacgtaa aggagacgat
       61 caagaagcat gtcagggcgc agcagatcgc aatggataac caccttccct gtgtttacct
      121 ggtcgattcg ggcggtattt tcctgcccga acagtcgaaa gtgtttgccg accgctttca
      181 tttcggacgg ttcttttaca accaggcgcg actttcggcc atgggcgtgc cacaaatcgc
      241 ctgcgtaatg ggattctgca cggccggcgg cgcctatgtg ccggctatga gcgatgaaac
      301 catcatcgtg aaggaacagg gaacgatcta tatcggcggg cctccgctgg tcaaagcggc
      361 gaccggcgag gtggtttctc ccgaagatct cggcggagcc tatgtccaca cctccatctc
      421 aggcgttgcc gactacctcg ccgaaaacga ccgtcatgcc atccagatca tccgtaatat
      481 cttccagacc ctcgagcgca aagaaaaaca gatcctggcg acatcggcca tcgaagaacc
      541 gtattatgac ccggaagagc tttacggcat cgccccgatc gatttcaaaa agcccgtcaa
      601 cgcccgggag atcattgccc gtatggttga cggaagccgt ttccaggagt tcaagaccaa
      661 ttatgcgccg acgctgatca caggttttgc caacatcatg ggctacccgg tggggatcct
      721 cgccagtaac ggtgttctgt tctccgaatc atccctgaag ggagctcact ttatcgaact
      781 gtgcacctcc cgcaaggtgc cgctgttgtt cctccagaat attaccggtt ttatcgtggg
      841 caaacagtac gaacatggcg gtatcgccaa agacggcgcg aagttcgtcc atgccgtggc
      901 caatgccgat gtgcccaagt tcaccgttgt gttcggcggt tctttcggag ccgggaatta
      961 tgctatggcg ggccgcggct acgatccccg gttgctcttt atgtggccca attcccggat
     1021 ttcggtcatg ggcggcgaac aggcatccca tgtcctgatc caggttaagg aggaacaact
     1081 caaagcccag ggcaaagaga tggacccggt ggaaagggaa aaactcaggg ctgagatcct
     1141 gaagaaatac gacgaagagg gttcccccta tttcagcaca tccagattat gggatgacgg
     1201 gatcatcgat ccggtcgata ccagaaaata tattgcaatg ggtatcgccg catccctgaa
     1261 ctgcaagttc ccggatacta aatacggagt gttcagaatg taatgttgaa tgttaaatgt
     1321 taaattttga attcgggtaa tcattttgat gatgaaataa tataaaggaa caaaccactg
     1381 atgtaaaatc aacctatgaa tacatacacg acaattgagt tccaaaagca gggcgacctg
     1441 gctaccgtat ggctgaaccg tcccgacatc cataatgcct tcaacgaagt gatgatccgg
     1501 gaggtgatcg attgtatcga agcggtgaac accatggagg atatccgcgt ggtgatcctc
     1561 aggggaaaag gaagatcttt ctgcgccggc gccgatctca actggatgcg cgacgtggca
     1621 aaatattcat acgagcagaa tttccaggaa agcctcaacc tgtcgaaatg tttctatacg
     1681 atctatacct gccggaaacc cactattgcc gtggttcacg gagcggctat cggcggggcg
     1741 aacggcctgc tggcttcctg tgattttgcc tatgccgaag ataacaccac gttttcattg
     1801 agcgaagtca agatcggcgt ggtccccgcc tgcatttctc cctatgtgat caaacgggtc
     1861 ggagagtacg ggtcgcgcga actgatgctg accggccgga ggatcaaagg caaagaggcc
     1921 gagcagttcc ggctggtcaa caaatcgctc ccggaagcgg aacttgatgc ttatgtcacg
     1981 tcggtgatcg atctgctcag aaccagcggg ccgaaggcaa tgacccagtg caaaaccttg
     2041 atatacaata tttgtaatat tgaaagcctg gaccaggccc gggaatcgac cgcccggatg
     2101 attgccgaga tcagggcatc ggaagagggg caggagggga tggcggcatt cctggagaag
     2161 agaaaaccga attggacaga tccttcgccc gccttcggcg gacccaggac aagcgactaa
     2221 cgactaaaga aatatgagga tgatgttatt cagtaagata ttgattgcca accgcggcga
     2281 gattgccgtc agggtgatac gggccgtgca ggagatgggg atgagggctg tggccattta
     2341 ttctgaagcc gatgccgcag cacttcatgt gaggatggcg gatgaggcct acagcctgga
     2401 aggcaatgac ctgtcgcaga cctacctgaa catcccgaag atcgtcggta ttgcaaaagc
     2461 ctgcggcgcc ggcgctatcc atcccggata tggattcctg tcggaaaacg ccgcatttgt
     2521 caaggcctgc gacgaggcgg ggatcgtctt catcgggccc aatgcccggg cgatgaagct
     2581 gatgggcaac aagatcaagt cgagggaggc gctgaaggat tcgggaatac caatgacggc
     2641 gggcattact ggcgatgtgg agaccctgcg gaagagggcc gccgagatcc cccttcccat
     2701 tatgatcaaa gcggcggccg gtggcggcgg gaaaggaatg cggatcgttc atgatacctc
     2761 caatctcgcc gaattgctcg aaagcaccag ccgcgaagca ctgtcttatt tcggcgacgg
     2821 caccatcttc atcgaaaaat atatcgaaga tcccaggcat atcgaaatcc aggtgctggg
     2881 cgacaattac ggcaatgtca ttcacctttt cgagcgcgaa tgctccatcc agcgccggca
     2941 ccagaagatc atcgaggagt cgccgtcacc cacgctcaca ccggagataa gggcgaacat
     3001 gggtgaaaca gccgtcagga tcgccaaaac gatcggttat aacaatgccg gaacggtcga
     3061 gttcctggtc gataagaacc tgaaattcta tttcctggag atgaataccc gcgtgcaggt
     3121 ggaacatcct gtgaccgaaa tggttacagg aatcgacatc gtaaaggagc agataaagat
     3181 cgctgcgggt aaaaagctgg agatcgggca ggaatcggtc aggcagaacg gccatgccat
     3241 cgaatgccgc atatatgccg aagacccggc caataacttt ctcccttcac ccggaaagat
     3301 gtcgtattac ctggagcccg aaggggaagg gatcaggctc gattcgggta tcctcgaggc
     3361 caccatgatc cacagtttct acgacccgct gatctgcaag ctgatcacct gggatgagga
     3421 ccgggaatcc gcccgtgaaa agatgatcgg ggcactcgat gaattcatta tacacggtat
     3481 ccggaccaat atcggttatc tgagacatct tcttaaaaac caggattata tatcgaacaa
     3541 tatttccacc aaatattgcg atgaatttac aggcaacctc aacggagaat tgttgtctga
     3601 gaaagataga atcccgctgt ttattcccgg gctggcttat tacctttacg atttcaattg
     3661 caagccggta accggagatc tgcaagggca cgatatttgg aaccacatca gctactggcg
     3721 cgatttaatg gatttcgaag gcgaagcgga tggaaaacct ttccgtttca ctgttgataa
     3781 gatcaaggat ggccggtatc tgataaatgc agccggagaa gcacatgagg ctcagatccg
     3841 tttttgcggt gagggctacc tggagatggt actggatggc cggcagcatg agttttatat
     3901 ttccacagat gaccagggca ccggatgggt aagttttgac gggtttatct tccatgtgaa
     3961 ccggaacgat atcctggcgg gaaaccaggc gatcctggga agcctcgatc actcgggcgg
     4021 gaagcaggat ggtaagatca tttcgcccat gccggggaag gtgatcaaga tcaatgtcgg
     4081 cgaaggggat gaggtgaaaa aagggcagac tttgctgatc gttgaagcca tgaagatgga
     4141 gaattatatc accgcgccca atgatgggat tatagaagat ataaaagtga aagtgggaga
     4201 tatggtgaat tcaaatacaa agctaattga aatatcttaa aaaatcccac cctgatgagg
     4261 gtgtctcaaa agtctattca tacgcacttt ggcctggcat tggtcaatgc tccaacgact
     4321 aaagccgttg gttccgaatg cgttataatt gacagaaccg acggcttcag tcgtcggact
     4381 gttaaaagta gacttttaat acaacctccc agggggtgaa ttcattaaaa taaataacga
     4441 tgaactacga cctgacagaa gaacagaaca tgatccggga aaccgtgcgg gagtttgccg
     4501 agcgggagat caagcccgta gcgaaagaac tcgacgagaa gtcggaattt tcggttgatt
     4561 tgaccagaaa gatgggtgag ctgggcctat tcgggatgta ccttccggaa aagtacggtg
     4621 gccagggcct tgattacctt tcgtacatca ttgccgtaga agagattgcc cggatcgaca
     4681 gctcccaggc ggcgacgctg gcggcgcata actcgctggg gatcggtccg ttgtactact
     4741 acgggactga agagcagaag atgaaatacc tgccgcagct ttgcaccggc ttcgcgctct
     4801 ggggtttcgg cttgacggag cccgatgccg gcagcgattc gcgcggtacc aaaacgaaag
     4861 cctacctgga gaacggggaa tgggtgataa acggatcgaa gatattcatc accaacggtt
     4921 cggtggatat ttcggtcgga tcaacggtgc aggccgtcac cagcgatact gacgggcgca
     4981 agctttacac ctgcatcatc gtggagaaag ggacgcccgg tttcacccgc cgcgggatgc
     5041 acggcaagtt gctgtggcgc gcgtcggata cggcggagct gtttttcgac gactgccggg
     5101 tgccggaagc aaacctgctc ggcaggatcg gggaaggatc gcacatcatg ctcagtacgc
     5161 tcgacagcgg ccggttatcg attgccgcga tgggcctggg actggcgcag ggagcatacg
     5221 agctggcgat gcaatacgcg aaggaacgca agcagttcgg gcaacccatc atcaaattcc
     5281 aggtaacggc tttcaagctc gccgatatgg cgactaagat cgagctggcc cggaacctgc
     5341 tgtacaaggc ctgctggctg aaagacaacg ggaagccgtt tggcaaggag gcggccatgt
     5401 cgaagctgta ttgttcggag atagcccgcg aagtggccga cgaggcggtc cagatccacg
     5461 gtggctacgg gctgatgaaa gactatgatg tcgaacgcca ttaccgcgac cagcgcctgc
     5521 tgcagatcgg ggaggggacc agcgaaattc agcggctggt gatctcaagg tatatcggct
     5581 gctaagaact tgccggctct cagaccggca ttggcccgca ggttaaaata atagaaagga
     5641 taatcgtaac tgtggtagac cccggggcca aagcccaggt ataaggtact atcgaccgcc
     5701 gtggaacaga tgagcacccc ctggccggta tcaatcctgg catcggcata ttgcggtacc
     5761 tgcgcaaaca ccccttcctg gtcgggcata tacgacccca acccttcggc agtagttgcc
     5821 ggggtttcat cccgggtcca tgagatcggg ttgatcacca gcccgacaat atcagataca
     5881 acgggatttt gaccgggact gacggccggg gattgtgtat tgtaagaaat gatcaccccc
     5941 gtatcgtcgg cgcgctcagc aaacttcagg tgtggattgg ctgtgagata ggcctgggtg
     6001 accgggtacc cgataacata cgccgcaatc atccgtgaat aaacttcagg atgttgtttt
     6061 aaataacctg aaaggaggaa caacaggatg ttggatccct gcgagtggcc tgccaggatg
     6121 aacggacggc catcattgaa gtgattgatg taatagtcga atgctgccgt ggcatctagc
     6181 aaaggaatac ctcctatcac cgaatccctt tgagcttcgg gcaggctgag cgtgtattgg
     6241 gcatctgcct ggcggtaata gggagcaaag atgtttcccg ttgttgcaaa tgccgttgcc
     6301 tgccggtgat aggccgacgc cgatcccgac agcatcatcg gattgtcgat gtcgcagata
     6361 ttgggatcgt caggatttaa tttttgccat gaagtcggat acaggtagaa tatgtccacc
     6421 tccttttccg gagccggaac cgaaagccag tgttccacca gggaataatc gacaggtgtt
     6481 gcatcaggca tgttgtcatc atctttattg cagccgaaac caggagctat gatcaggaac
     6541 agagcgggaa gtcctggcag taagtaaaat agtttggtca ttttcatctt tagaattatt
     6601 gaagccttta aagtattatt ccataattca ggtaaaagta attccataat tttttgatga
     6661 gggagatgct gttacttgtc cagccatccc aatgctgcgg taggatcggt tgttaccagt
     6721 atttttaatc ccctgttgga agcgactgtc tcatagaatt tgccttcctt taatgatctt
     6781 ttatggcaga ttatcgctac tttactgaaa tatgaggcat gttgagaggc gttttcagca
     6841 agcaaataag tgtcggtcgc ggtaatggcg tgctgaaggt gcctttcatc acagaggatc
     6901 tttgtgcagc catgttgcct ggatgcttca atgatcgtcc gggaataatt catcacttct
     6961 tcgaagctgt cgtctttgcc atgagctgtc acttttaaca gttcatcctc aacggaaaac
     7021 acatacttga taggcatggt ggattgattt aagaacttta agtgactgaa aaaataaccg
     7081 gccggactct ggcaaagtca gacctttcgg ggggcatttg ggtttttttc caaaacaaat
     7141 ataatggata aattatataa atcaatgatt tcgtgataaa aatatctgaa caggcttttg
     7201 ttactatcgt atctgttcgt cgaatggccc gtcaatcaag acattttacc cgaccggttt
     7261 aaaaaatccg gattggactt gtatttcaga agtgttattt gctaatttta tgttcaaaga
     7321 ttgattaaac caaaaggaaa tgaaaaaatc atttattgca gtctttattt tagcaggcgc
     7381 cattatttca gggcattcac aaagctttac ggcgtatatg ctggtagctc ctgaagcata
     7441 cctggaagat gtccataaga tttccattct cgattttgac ggaaggatgg ggacagccat
     7501 gaccgattat ctcgctgccg aactgatgca ggagttcagg ggcacaggcg gaaaatcggg
     7561 cggaaactac ttttctgcag gtaaatccat taataatttt cagaaaggag cccggaccaa
     7621 tgttttttca ctggtggagc gcagtgaact ggataaagtg ctgcgggaac aaaatatcag
     7681 caattcgggt ctgatcgacg actcccaggc cgcccagatc ggtaaacttc tgggaataga
     7741 tgccatgatc accggttcag tggcctattc acaccaggat gagcagaaaa cccttactta
     7801 caccgacctg aacgggaata aaaaatactc ttactgtacc acccgaaccg tgacggcaga
     7861 agggcatctt aaaattatca atgtcaatac cggtcagatc atcgggacta ccgtgaaaac
     7921 cacctcctac tccgataaga aatgcgatga taaaagatcc acattgtttt ctgtcgaaga
     7981 gctggcaaaa gtatgctatg atggccttgc caccaaactg gccaattatt tcaccccggt
     8041 atttttgcct atggaatatg agtttgagaa gattaaagat aaggagtata aagaaaaagg
     8101 caaactggcg atggattatc tcgaaagcgg cgacctgaac aatgccttct ccatttataa
     8161 atccatttat gacatcgatc cttataattc agcggcttca agcaatatcg ccaggttgta
     8221 caggatagcc gggaattatg aaaaagcccg ggaatattat gccctggccg ccgaggtcga
     8281 cgaagtaacc tatgctcccc tggtgcaggc tgcaataaga gagaaagaga tgcttagcct
     8341 gttcgaaggg tatggcataa atatcgagca gaccgatttt atccagaaat cggatgccct
     8401 ggccgaaaga gtcacaacgc gaggcaacaa atcggataag tacgacgtta gggtaaatcc
     8461 tgaccaaacc tcggacgtcg tggccaaagt gccgggaagc actgaattta ccgtaattga
     8521 aaagcaggga gaatattatc tgatcaaact gctcggaggt aaacagggct atatccatga
     8581 tgattatgtt aaatgaagat gatgttagaa ctgccttttc atgtgcagtg agatatttta
     8641 atggaatcca ttatcactga aagcgatgaa gtacctgaac ccgatccttt ttttcgtttt
     8701 atttcctttt cttgccaccg ttgcccagcc ggaaatccct gcctggtgcg ataagagttt
     8761 cagggaaagt cattatcccg ccgaccagta ccttacaggt tatatggaag gaggaaagat
     8821 tgtcaatgaa accctcgatc aggcccggga gaggatgaag aagctggcgc agagcgaact
     8881 gatcgaaggg gtgatggtcc agatacagtc ttatacggag agcaaagtgc aggatatcac
     8941 ccggcaatca acttccagtg tggtccagtt ttatgaagcc tctgtaaaaa cagagagcaa
     9001 tatggagctc acaggcctga ccaacgaggt gttctataat cctgaaacag ctaccgtata
     9061 tgctttcagc tatgccaacc ggtatgagat catcggttat tacaaggcca atattgggtt
     9121 ttaccagcaa cagatcatcc aggttgttga attgagcgac cggttgtaca cagatggcga
     9181 gaaggtgaaa gcccggtcgg aactcgagaa agccatcccc ctgtttgccc ggatcgatta
     9241 ctcgtatggc gtgctgctgg cccttaaagg gaacatcgat tcgctgcagt acgccaattc
     9301 gatgcaaatg aaatcgacgg ccatcaggaa actggccgac ctggaaaaag gggtcatcgt
     9361 ttttgtacag agcagtgaag atatatttgg ctccccttcg gagattttag ccaatgcgtt
     9421 gaaaagggat ctgaaagggt gcagcttcac cgccgacgag tcggccagcg actggaaagt
     9481 gacgatagat gcaaaagcaa gggaatattc ttacacgaat ggtttttatt tcagttatgc
     9541 ggatgtggtc ttatcgctga tgaaaacccg gacgggccag ctgctttacc aggaggagtt
     9601 ttcgcagaaa gggggagggt ccgattaccg gaaagctgcg cgcaaggcga tggaagaggc
     9661 agcaacggcg gtatccggaa agatcgtggc agaaatcaat aaatagcgat cagtcaattc
     9721 atgaacccgc ttaagaaact ggcagggcag acacttgtct acgggatggg gactatcgtt
     9781 cccaggctgc tgaattactt cctcctcacc ccgttctata cccgaatctt catcaccggc
     9841 gaatatggtg tcatcaccga gctgtatgcc tacatggctt tcctgctggt gttgctgacc
     9901 tacggcatgg agaccgcctt tttccgtttc gccggaaaag agcccgatcc gaaaaaagtc
     9961 ttcagcacct ccctcgtctc cctctttgta acctccgtgc tctttgtgtt cttagtggtt
    10021 atattcagcc agcctgtcgc aaccctgatc gaatacggca acaacccgga atacatcacg
    10081 atgttctcgg tgatcgtggc cctcgatgca tttacggcca tcccgttcgc ttacctgcgg
    10141 cacaggaaca aagcgctgcg gttttccatc atcaagatcg tgaacgtgct ggtcaatatc
    10201 gggttgaact tctttttcct ctggctggcg ccgaagatgc tcagcgccaa cccgggctcc
    10261 tgggtgcggc tggtgtacga ccctgccatc ggcgtagggt acgcgtttat cgccaacctg
    10321 gcctcaagcg ggctcaccct gctatagttg ctgcccgata ttttcagtgt caggccggct
    10381 gtcgacaaag accttttaaa acggatgctg acctacgctt tcccgctgct gatcgtcggg
    10441 ctggccggga tggtcaacga agtggccgac aagatcatct tcaagttcct cctcatcgtt
    10501 ccgcccggcg aggctgatcc gaaggcctat gccatgagcg aactgggcgt ttacggggcg
    10561 gcctacaagc tttcggtgct gatgacgctg tttatccaga tgttccgcta tgccgccgag
    10621 cccttctttt tcgcccagaa gaaggaggag aatgccaaaa agatctacgc cgatgtgatg
    10681 aagtatttcg tcattttctg cctgatcatc ttcctgggtg tgaccctgtt tgccgatatc
    10741 gtcaaatact tcatcgggcc cgactaccgg gaagggctgt tcatcctgcc catcgtactg
    10801 atggctaacc ttctgctggg gatcaccttc aacctgtcga tctggtacaa gctcaacgac
    10861 atgacccgat tcggcgccta tatcggcctg tcgggcgccg ccatgacgat actgatgaat
    10921 gtgctgctgg taccccgcct gagttatctc ggtgcagcaa taggtcatct ttccgcctac
    10981 agtcttatgg tcatactatc ctattactgg gggcagaaat actaccggat cggttacccg
    11041 atgaagcgga tcgggtttta cacaattctg accctggcgt tattccttcc cggttactac
    11101 ctccctgtcg ggaaccaggc gctgtcgctg gcgatcaacg cggttctttt ctgcattttc
    11161 ctggcggttg tctattttaa ggaaagacgg gaaataaggg ggttataagc tcgctgcgct
    11221 cgcgtcattg gctcactgcg ttcgcgtcat tggtttattg ttttacgccg gtgacgtagt
    11281 agttgtttcc ggctccgagg ccgcctaatc cgccgacgct gaaaaagatc gaatcggtgg
    11341 tggtaaatcc tccgctatgc cagtagtgat agccgccctt tggaagtaat gcgcctgctg
    11401 tatctaaaac agaggtgatt ttcgccgaag ggccgaaaat gatcgtcact ttcgtatcag
    11461 gaacctcatt gatgtcatcc tggatgtaat agaggtcatc gatgctgtcg gcggggtcaa
    11521 atctccggat aataccggca tacacggaag tatcgtatgt gttcggctgg cccatcgacc
    11581 aggattcgtt aatgaccgta aaccggaaat cacccaggaa tttctccctg tagtcgaaga
    11641 tctctatttg aatttcatcc tctcccgttg cgccgtcatt atcataagca atcgctctga
    11701 aaagataatt accggcattc tccagcttcg catcaagcca ttcataaaca tacggtgatt
    11761 caagatcagt cgatactgtg gcttcgttta tataaaactc aactttggta atattcccat
    11821 ccacatcttc cggctcagcg ataaaaacca ggcttgtatc atgggtgacg accaggagac
    11881 tgtcgggttg cgtgaaacgg atggtggggg caatattggt cttgtcctta ttgcaggaca
    11941 cagcaatcag cataagtgcc gcaaccgaaa taatcaataa tcttttgatc ataaaaattg
    12001 actaagttct ttttaaaaca caatcagcat aataaaatat aacggaatgt ctgttgtaat
    12061 attgggcatg ctgcgaaaag tcaattttcc ggaagatgcc acaaaagcac caatacacca
    12121 aatggcacag aaaccgggtt tccaaccctc atatcaatta caggaacacg aatgaaacga
    12181 attgaacgga tgatcacgga ttgtaaaatg gaagccgaat ggatacgtaa agttgccgat
    12241 ccccgattca agagaaataa aacctaattt tgcaccctca agacaatcct gaaacaattg
    12301 aacaattgaa caattgaaca attgaacaat tgaacagttg aacaattgaa caattgaaca
    12361 gttgaacagt tgaacaattg aacaattgaa caattgaaca attgaacagt tgaacaattg
    12421 aacagttgaa caatgaataa tcactccgaa ctatgaaaag tgtattgcgt cttctgatct
    12481 ttcttactgt cctacagtct aacagtcctt taatccatgc ccaggggcag ttctcgaaga
    12541 accgggagac ctattacgaa gcgaccaaac agaaaatact gggtaatttg gacaaggcca
    12601 ttgaactttt caaaaagtgc atcgagctgg attcgaaaga tgcagcggcc atgtacgaac
    12661 tggcatcgat ctataccgac ctgggaaaac cggcagaggc attgccattt gcggaaaaag
    12721 ccgtagcagc cgacccgtca aattcctggt ataaaatact gctggtacgg atcttacagg
    12781 cacagggaaa atattccgat gcagctaaaa ctatcgatca gttactggtc agcgatccgg
    12841 agaatatcga ctattaccag gaccaggcgc tgaactacct gtatgacggc gattacaaga
    12901 atgcggtcaa atcgtacgac atgctggaac agaagatcgg tatcaatgaa gatctttcgg
    12961 tgcagaaaga gaagatctac ctgatgatag gtaaaacaga caaggcgatc gaagagatcc
    13021 ggaaactcgg ccaggctttt cccgatgagc cccggtacat ggagatgctg gccgagctat
    13081 acatgaacac cgggatgtat gaccaggcgc tcgagctcta caaccggatc cttgaagccg
    13141 acccggataa tccttacatc aacatttcgc tttccgacta ttaccggaaa acaggcaacc
    13201 gcgacaaagc ttacgaatac ctgaaagcag ggtttgctaa cccggccctc gatatcgatt
    13261 ccaaagtgtc gatcctgctg gcttattaca gtgtcacgga tatctacagc gattacaagg
    13321 aggaggcgtt tgagctggct gagatcctca tccgggccca tccgaacgat ccgaaggccc
    13381 actcgatcta tgccgatttc ctggtgcagg acaagcggta tgcagaagcc cgcgatacat
    13441 tccgcaaagt gatcagcctc gacagcacaa agtacctgat ctgggaacaa ttgctctttg
    13501 tggagtcgga gctgaacgat aaccaggcga tggctgccga gagcaagcgg gcgctggacc
    13561 ttttccccga tcagcccatg ttgtacatgt ttgccggggc ggcccatttt atgcttaaag
    13621 attatgaggc ggcggtaaag tacttcaggg ccggcgttaa tttcgtagtg ggcaacgacc
    13681 ggatgctgga gcaattctat tcctacctcg gcgattccta tttccagctg aaagatcacg
    13741 agaaatcgga tgaggcttat gaaaaggtgc tgaagatcaa cccggccaat acactggtgc
    13801 tgaacaacta tgcctactac ctgtcgttac ggggaaaaga cctggagaag gcggaacaga
    13861 tggctgaaag ggccgtggag atggagcccg acaacagttc ctacctcgac acctacggat
    13921 gggtgctgtt taagctgggc cggtatgaag aagccaaacc atggattgaa agagccatgg
    13981 aaaaagggga aaacagtgca gtcgtcctgg aacattacgg cgatgtactg tggaaactga
    14041 atgataaaaa agaagcggtg aaatactggg agaaggccct ggatgccggg accgggtcgg
    14101 agttccttga aatgaaggtc cgggataaag aataccatga ttaagatttt taagagaata
    14161 gggatgatgg gcctggtgat cctggcgctg tcatcgtgcc gttcgacacg gccactgatc
    14221 aaagagccgc tgaaggaaca ggggccggag tacctttaca gccggctgaa acagagcgaa
    14281 ttccggttcg accggctgtc gatgaagttt acagcctcgt attccgacaa aaagagctct
    14341 gccgacctca acgggcagat caggatcagg aaagacagcc tgatctggat cacggcatca
    14401 ccggccatgg ggctggagat gttccgtatg gtgataacca ccgattcggt gaagtacctc
    14461 aaccggatga acaaagacta ttttgccggc gattacgcac tggtcagccg gttcctgcaa
    14521 atcgacgttg atttcgacat gctgcagtcg atcctgctgg gaaacgattt ccagtttttt
    14581 gagacgaata atttccgggc ttccatcgac aacctgaatt ataaactttc cactaccgga
    14641 aggcgtaaga tcaggaaaga agcggaatcc gccaaaaatg acccggtcat tcttttgcag
    14701 aacatctggc tcgatccgtc atcttacaaa ataaaaaaaa tagacctgaa ggaatatatg
    14761 aaggataacc ggaagcttac agccacgtac ggtgattttc aggcccttaa cgatcaaatc
    14821 taccccagtg atatccgttt cgagatccag gccgaagaga aatcaaaggt cagggtgaaa
    14881 tatgataaag tagccacggg agagcaaatg accttcccct tctcaattcc cccgaattat
    14941 caaaggatca ggtgatttat tcctattttt gtttcatcga taaaattctt tacggaaccg
    15001 gatgaacaga ttcacctttt tctttatcct tttgataatg ccttttacgg ttgctttcgc
    15061 ccagaaggac cgtacaaagc tggagcagga caagaaaaag atcgaagagg agatcgaata
    15121 caccaacaag ctgctggatc agacacggca aacccggcag aactccctca atgaggtggt
    15181 tatccttaat aaaaagatca gtcagcgcga acagcttatc aacacgatca gcagcgagat
    15241 cgggctggtc gacgggcaga tggccctgac cctcgataca attaaattgc tggaaaacga
    15301 cctgaagagc ctcaaagacg aatatgccca gatgatctac tatgcatata agaaccagaa
    15361 tctttatgac cggatgctct tcatcttttc tgcaaaagac ttcaaccagg cctatcagcg
    15421 gctgaaatat ttcgaatttt acaaccagta ccggcggcag caggcgttgc ttatccaggt
    15481 aaaacaaaag cggctggaga taaaaatgga ggagctggcc gctatcagaa cgcagaagaa
    15541 caatctgctg accggggaag agcgccagcg gcagcagctt accgtggaac gggaggagaa
    15601 gaacaaatcg gtgaaagccc tttcgaagaa ggagaaggag ctgcagaaga cgctgaaaga
    15661 gaaagaggcg gcggcaaaga agctccagca ggccatccag gagatcatcg ccgaggagat
    15721 caggctggcc aatgaaaggg caagtaaata cggcgccccg ctcccgaaaa cgggcgttta
    15781 tgcgctgacg ccggaagaaa agatcatttc cgataatttc cagagcaata aaggcaagct
    15841 gccctggccg gtggaacagg ggatcgtatc gggcactttc ggagagcatg cccacccggt
    15901 acttaagaat gtgacgacgc gcaacaacgg catcgacctg ctcacccagg ccggcaccga
    15961 agcccgggcc attttcagcg gcgtcgttac ccgcgtgatg agcgtcccta acaacaacaa
    16021 cgtggtcatc atccgccacg gcgaattcct ttcggtctat tccaacctcg accaggttta
    16081 tgtgaagatc ggggagaagg tgacgacgaa gcagaagatc ggcacggttt tcaccaacaa
    16141 ggaagattca aaaacggaac ttcatttcga agtgtggcag tcgaaaacac tccagaaccc
    16201 ggtggaatgg ctggcaaaat gagttaaccc cacatttacc ttgatatcaa gatcttctgt
    16261 gaatcaatca cctggtcgtt ttctttaaga atggccaggt acatacccgg tttaaatgaa
    16321 cgggcatcca ggatcagttc attctgattc gacccggaat tcaaagtatg atggatcctt
    16381 ccgtcggcgc tgaccatatc aatggaataa acactcccct cacttacacc cgcgaccttg
    16441 aacctgataa cgtccctggc cggattgggc cagatggcca ggcttttcga taacctgtcc
    16501 ggatttcccg acagatcatc aagaccgata aaggggccga tcaggagatg gacggggacg
    16561 ttaaacaggc cgtcgttgcc gtaattaaag gcgatatagg tctggagtgt atcgtaaggc
    16621 attccggtgg tgttgaagtc gacctggatg ctgccggtac cgaatccact gagggtgccg
    16681 gaggtggcgc cggtgagagc gatccacggg aaggtgcctg ccagggcgat ttcataacta
    16741 acactggacg ccgagttatt ggtgaaagag aggatcacgc tctcctgctc gttgggctgc
    16801 atcatctgga ggaggctcat cgggtcgaag gagatggcgg gattgagcag gagctgtgcg
    16861 gcgttgtaaa tgttgaggcg tccgccggag accgatttat tggccagggt aggaatcggg
    16921 tctgcgccat tgagaatata ttgtttcaga acgagggcca tgccggccgg atcggcgtga
    16981 tacgattcca taaaggattc atcagcaact gaaaataggt aagcgatggc gccggccacg
    17041 tgcgggctcg acatcgaggt gccggtcttg aggccgtatg aattgttata ccgggtgctg
    17101 taaacctggg tgccgggggc gccgatgtcg atggtggtca ggccatagcc ggcatagggg
    17161 ctcagctcgt cgtcactggt ggtattggtc accgtaatga gcgcttcgtt ggggaaggcc
    17221 gtaggaatat cgcccaccac atcgatattc aggttggcat tgactgtagc ggccgcattg
    17281 agaatgccct gcatgcccat ggagtcgtac atggcgctcc agatcgggaa atcttcgatg
    17341 tcgccatcgg tgccgaaaga ggaattggat gaaacgatga aggcgccctt tgcgccgtcg
    17401 gttttattgt agagccaccg catctcgtaa acataggagt agcctcccac cacgatcgat
    17461 tcggttgtcg ccgagcccac caccggcatc acttttacgt tcatgttcac gccggtcacg
    17521 ccgaggccgt tattgccctt tgctgcagca atgccggtca catgcgttcc gtggtctttc
    17581 tccaccaggt tgccggagtt gttccacgaa ttccagccgt ggtaatcatc gatatagccg
    17641 ttggaatcat cgtcgatccc gttgcctggg atttcctggt agttcttcca gaagcggata
    17701 tcttcatgtt ccaggtcaaa tccgtcgtcg accacggcaa tgatgatcgt atcgccggtg
    17761 gcggtaacac ctgaagtgct tatgccccag gcttccggcc cgtcgatatc ggcatcgggc
    17821 tggccgccgg tctgccctgt gttgttcata ttccattgct gtgaaaaact ggggtcattc
    17881 gggatgaatt ccctcagttc gatgaaatgg ttgaactggg ccagttcgac aaacggatgg
    17941 gatttgatct cctggagcat ccggtcgtca ctgatcaccg acggatcgta gctgaggagg
    18001 aagatattca ggcggtcgga gagccggcgg ataacgccaa gccctgcgcc ctggaaatcc
    18061 cccagcacat cctgcagcat ttgctgctgg ttctggggaa ggtcgctgta tagcttgatc
    18121 atgatttcac cttcggcata gggtttttcg tgcttgttgg cgggggtatt ggatataagg
    18181 aatggtatga taatcaggaa gataaagaaa gatgtgattt ttttcataag acagggaatt
    18241 tgagggggta aagataagat tatttctgat gatgggtgtt gggtttattc accacagagt
    18301 aggaagttgt ctcaaaagtc catatattca cttttattta ggtagtccga cgagcgtccc
    18361 gtcgattcct gagggaattt ccttccgacg actgaaaccg tcggttccgt ctttgtaata
    18421 ttctccgaac caacggcttt agtcgttgga gcacgaactg ttttcaggca atcaaatcga
    18481 aatataagac tttttagacg gtctccttga tgaattttaa agtgccggaa agcccgtttt
    18541 catcttccat ccttaaaaaa tacaatcccg gactaagcgc tgaaatatcc aaagtaaatg
    18601 gaaacgattg aatatcttct atttgcatta cggtttgccc gaaaggagtg atgacggata
    18661 accgggctgc gggccgatga agatcaatgg ccagaatgct tgtagcagga ttgggataaa
    18721 tcctccaacc acttatttta tctgtttcat ccgaaattcc tatcgaaccg tcagtgatgg
    18781 taaagggcgc tgttgtcatt ccctgggcat tcgattggcc ggcatacatc gagtaccgga
    18841 tgtagcagtt atccgagttt tcctggggca ctatccactg gtagctcccg ttgtcgggca
    18901 gttccgcagc gatcggaatc caggggccct ctggccctgt gaccgaatat tccagttgca
    18961 caaaggattc ctggttaccg taaacggcgc tgatccaacc gatgttcctg accgatcccg
    19021 gcttcagcaa ttcatggccc cgggggaaaa ccggccggat gtccaatgcc aatggtgttg
    19081 agttttcttt gaaaaagtgc aggaaattct gataagtaaa ccagctcaga ttggtctctt
    19141 caagtaaaac tacatcgggt ttcccgttat ggtcggcatc gccgccaacc ctgaacgcct
    19201 ggcagcttcc cggatcacct acggaaaaag tagcgtcggc agtccagttg ccctgcccgt
    19261 tgcccaggaa cacccgaaag gtacccgtcc cgaacgccgc aaggtcaatg aagccatcgg
    19321 tgttcatgtc ggccagctgg gtcatttcaa aatctccaga agagggcaga ttccctgaaa
    19381 agtcggccca ttcgccgata tcttccataa aagtataaac gtgcaaaccg ccgtcatagt
    19441 tcgtaaatgc caggtccatt ccaccgtcat tatccgcatc acccagcgaa ggcccgctgc
    19501 ggccaatgtt atcgagtgaa ggaaggttca ggtcattgat gctgaaaccg cccgttccgt
    19561 ctccgaagta agctgttccc gattcatggt ccacgatgaa atccatgttc ccatcgttgt
    19621 tgatatcccc gaactggaag atcatcgagg aattgcccgt gttgaaccca aagctctggg
    19681 tccatgtccc gtcgaggttg ttcaggtaaa catggatccc ggaacagcac ccaaaggaga
    19741 tcgagccgat atctaggtcg ccgtcgttat ccacatcggc aaaatcggta ccgaacatgc
    19801 cccagtcttc cccattggtt gccagcccgt cgtcccaggg tatccagccg gtcccggtgc
    19861 cgtttcccag agccacttcg atcagctggt cgccgagatc ggtggaagaa taattgtggt
    19921 gcatgccgta gccaatatcc atcaggccgt cgttgttgac atcgccggcg gcaatccccc
    19981 cgtatccgag gtcgccgccc atttgcacct cccagttccc ctgcccgtcg ccgaaataaa
    20041 ccattatacc ctgctctccc gattgtatcc ccggatttcc gtgatcacca atggtaatga
    20101 tatccgggtg cccgtcgcca tttatgtcgg caaattcaag ctcgctccgc cctccttccc
    20161 attcgggact ggccaggccg gaggaggatt cggtgtaggt aagcaatgtg ggggccaacg
    20221 atttattgac ttgcgtaaat gcctgtaata cagtaccgca aagtaggatc accaggtagc
    20281 tggtcatggg aatctttttc atggattagt tttgtgcttt gatgaatttt gaagtatcgg
    20341 aatgcccttt ttcatcttcc atccttaaaa aatacaaccc cgggctgagc gctgaaatat
    20401 ccagagtata tggaaacgat tgaatatctt ctatttgcat tacggtttgc ccgtagaggt
    20461 taatgacgaa aagtgaagtt ggaattacgg gtgacgggtt acgggtgacg ggatcctgga
    20521 tattgatgtt aataaggttt gtggcaggga cagggtaaat atgcagattc attacttctt
    20581 tttgaccgtt gatttcatcc gtaccgatcg gactgacgtt gatgccgaag aattccaggt
    20641 aacgggtcag cagttcagtg gaggcgccgg aagggatacc gggctgggtg ccgaattcga
    20701 acagggcgcc gatagttttg taattgccgg catcgtaggc aacggcgcag gggagtcggg
    20761 tgctgttgtc ctcaaggatg ggaaaagccg gcgggatcgc gtcgaggtaa taaaaactga
    20821 agggatgaat ggcttcgtta gtaaaggtaa gaccggctgc aaatgcagta tcaatcccgg
    20881 taatggtatc gaagggggta atattgcctg cccactggat attaaatttc ggctgggccg
    20941 gcgttcccgg atcatctttc caggttgtcc ggctttccag gtaaactttt cctcccgcat
    21001 ccaggtaatt ggcgatcagc gtgccttccc ccagggtcag cagatggttc tggtaatagt
    21061 aacccaggca gataaacaag gattcgtaat caaatatttt gggtgtgatc gaataatcgt
    21121 attcagaaag aacgttgagt tcattcaaaa gcgaaaagat caccgggccc gaatttttgt
    21181 cgacggccat atcgatcacc attaccggcg ttttcccgac tttcatttcg atggtatcct
    21241 gttgtccgat tcctgatgct gatgttgtgg tcagaactaa tgtgaccggt gtgccgctgg
    21301 gagtggaatc atcggcagtg agcgtgaagg accgcgtaac agacgcacct ttcccgatgc
    21361 tgccgaaatc ctgcacagta tttccggata ccaccacttc gggactgagg gagaccagct
    21421 ccgacataac ctgatcgatt gcggcatggc ccgaattgcg caaagtggcc agcagtggcg
    21481 ctgtttctcc ggggctgaga cggttgtctt cgtcgccgat ctcattcctt tcgagaaaga
    21541 gtccgggtgc gttggattga aggacgagat ccttgaccca gtttcggccg gaggcatcga
    21601 gcactgtttc gaactggata tcgctatggt ctggaataaa agtggatact acgaaactga
    21661 aagcatctgt caccgacacc gattcaccgg caggaatcgt ccccgtctgg cactgcgaat
    21721 ccaggaaaat gatgaacgga tcgctggtcg aaaggctgat gaaaggattt tcgaggatct
    21781 ccgtgccgcc gttgaccagg gtcagttgca agcttaccgt atcgccgtag aagacctggt
    21841 catcgtttcc cggcattacc gaaagataaa tgtaaggtcc agaagttagc tggaagtcgc
    21901 gggcgacgga tattcccgat cggtcggtca ccctgacggc cacatcatgg attcggtcgt
    21961 cggaatgggg tgtccccgac aaaagtccat tttcggaaag ctgaaaagtt ccggggagtg
    22021 actgatagcg gaatgttaca ctggttccgg gtggtatttg tggctggcca ctgtatggcg
    22081 aaaccaggta gtctgttttg ttaccggccg ataatcctgc cagccagccg aatccgccaa
    22141 gcgttgaagg gccgtatata aatccgatag taccatcctc ttccagtttt acggtgaaat
    22201 tgatgtcggt agtgcccagg ttgccttcgg ctgaaagctt ccagcggaaa atggcggcag
    22261 tgtcgctggt ttcgacccat ccgccatcgc
//
LOCUS       DAOPNH010000012        21427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2305_length_21427_cov_6.216405, whole genome shotgun sequence.
ACCESSION   DAOPNH010000012 DAOPNH010000000
VERSION     DAOPNH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_21427_cov_6.216405"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKZ74_01495"
     CDS             complement(<1..1160)
                     /locus_tag="PKZ74_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM91451.1"
                     /translation="MRKKLLLINPANELRQGFTNDDSTRFMPIGLGIVAALTPNHWEV
                     ELIDESYERFSFRFADLVAFTGFTANAPRAYKIASVYREKGIHTVMGGIHASLATDEV
                     AEYVDTVFTGEAEGAWQGLIADFEAGKIKKLYDGGNVDIHSVPHVRRDIYTYPYVYDL
                     VSTSRGCPLGCEFCSVTQLCGKTYRERKVEDILDELEETTRPLIFFVDDNFIQNTKRS
                     HERAISLFRGMVERKLNKFWVCQATVNFAENEEVLHWARKSGCMMVFVGVEAENEEAL
                     KDVRKNLNLKRGVQSYEQTFRKAHKHGIGILAFMIFGMESDKKEDLFARRDYILRSSL
                     DAYQCAIMTPLPGTVLFERVKARNQIVLNNYPDDWQQYDCHAATIDTASMDHA"
     gene            1269..2975
                     /locus_tag="PKZ74_01500"
     CDS             1269..2975
                     /locus_tag="PKZ74_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM91452.1"
                     /translation="MIASIRNRKTLADLLISAAGESPENGVGFIRADGSIQFLTYPGL
                     LAEAETIASGIRSEGIKEGESVMIIITRNEVLVPTLWGCILAGVVPTILQPPVSFTEV
                     NPPASKIENVYSILDKPGIILSDDLAGSFNSTVISPESIIPASDLAAGKNPVRQELSN
                     ETENLAFIQFSSGSTGDPKGIMLTHRNILVNIAAICVGLDIHKSDVMCNWMPLYHDMG
                     LFGFHICPVFAQSNHYLIDPVEFIKKPTLWLDVIEHSGSTITGCPNFGQALLLRYLKN
                     RQESGRNLSSLKAIVNGAEPISNRIMSEFTGKLSVSGLRKEVMMPAYGMAENTLAITF
                     ADLWSEPLVMNFNRTLLQAQGIAVREEKKSGETVELVSVGKALNDVEIKIADDDGNGL
                     AEGMEGHIRIRGKSVTSGYINNPEATAEAFEDGWLKTGDKGFFYDGQLYITGRIKDII
                     FVHGQNLYAHDLENLASKHADIPYGKVIIGGSFDPKKGKDQVILFLVGSANQALCDLF
                     LELKGFFRDTYGVHIDVFVPVRSNQVPKTSSGKIQRYKLLEDYRDGVFDEAIAEIRRL
                     MR"
     gene            3519..3755
                     /locus_tag="PKZ74_01505"
     CDS             3519..3755
                     /locus_tag="PKZ74_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPM91453.1"
                     /translation="MTIEDFIARIEEEFDDLEPGKLRPESNFREVFEWNSINALILIA
                     LVKTNYDVAINAEDITKSKTVNDLYKIVKERIAS"
     gene            complement(3811..4053)
                     /locus_tag="PKZ74_01510"
     CDS             complement(3811..4053)
                     /locus_tag="PKZ74_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPM91454.1"
                     /translation="MTWIHLKLKIRLEKIDLSKPITVYGLNSMKAVQLQQDFLENFQV
                     NFPPYLFFERISLNELSERAFRLIKESETPYRNRRL"
     gene            complement(4477..5913)
                     /locus_tag="PKZ74_01515"
                     /pseudo
     CDS             complement(4477..5913)
                     /locus_tag="PKZ74_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            complement(5958..8015)
                     /locus_tag="PKZ74_01520"
     CDS             complement(5958..8015)
                     /locus_tag="PKZ74_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM91455.1"
                     /translation="MEDNFFNDEFLPAGVIKDPSLMHILQGRAVSNPDQTAFIFIQDG
                     DESEEHITYRELHQAAIDLSHSLLEIAKPGDRALMLFPPGLDFIKALFGCFYAGVIAV
                     PAYPPRKNRSLERLRLLVNDSGSSLVLSTDDIRQSAERSFADVTELQGIPWITPNPPI
                     PQSSNLQISNLQISKSPNPDIALLQYTSGSTGHPKGVMVTHRNIIRNAEYIRQSFALS
                     PKSVSVTWLPSFHDMGLIDGVLVPVYTGFPGVILPPVSFLQKPVRWLKAITKYRGTHG
                     GGPNFAFDLCAEGIAEEEKISLDLSSMETLYCGAEPIRKSTFERFVSSFREYGFSHEK
                     LYPCYGMAETTLIITGPHAGRGPVYLCLSAGELEKNRIVPVSDQHPDARHLVGVGYPW
                     IDTRVKIVDPETCRLLNDDEVGEIWVSGSIVTAGYWGKPDASKEVFAAEIAGDPGEKY
                     LRTGDLGFFHDGELFISGRYKDLIIINGRNIYPQDIEFLTENSHPSIRKNASAAFPVD
                     ISGEEKLVVVAEVERTAIRDLDVDGVCDRIRENIVMETELSVHAIQLLRTASILKTSS
                     GKIQRKACREAFLQNKLDSLGESVLETQFHEDQPEPTSVDIVNLEAWLMTWIHEKLKI
                     PLNRIDLARPITAYGLTSMKAVALQQDFLERYSVSFPPYLFFEKSSLRELCEKAIKII
                     KEN"
     gene            complement(8058..10142)
                     /locus_tag="PKZ74_01525"
     CDS             complement(8058..10142)
                     /locus_tag="PKZ74_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM91456.1"
                     /translation="MGLENFLYDRITEADTNTFSLLDVLNYRVSNTPDEIAFIYLKDG
                     ESDEERITYRELGSSASGLARRMMEMNVAGKRALLLFPPGLEFIKSLMACFYASVIAF
                     PAYPPRKNRSLDRIMKIVTDSEATTVITTTEIYQSFNRSFSDLTELRSLEWIVFRDNP
                     DLHENLESYIPKKTRQVDPHSSSFPDPDSVALLQYTSGSTGNPKGVMITHRNIMRNCE
                     FIRNSFGFSRKTVAVSWLPTFHDMGLVGQIFEPVYTGFPSVLMSPVSFFQKPVRWLRA
                     ITKYSGTMGGAPNFAYDLLTESVTAGEKQELDLSSLKTIYCGAEPIRKSTFDNFFNAY
                     KAHGLREEMLYPCYGMAETTLITTGPPAGRRPKYLAVDGESLKKNKVRLPRNSESEVK
                     CLVSVGFPWLDMKIRIVDPETLTPCMADEVGEIWISGSSVSPGYWNKAEINREVFQAR
                     LDDDPGTNYLRSGDLGFLHEGELYVSGRLKDMIIIYGRNIYPQDIEFIAENSHPALRQ
                     NSSAAFSVEVNGEEKLVIVAEVERSAIIGLDVDAVCDSIRENVALETELAVHAIQLLR
                     TASILKTSSGKIQRRACMEAFLNKTLDVVGESYLESQYHTDEPDDTTVDIVRLEAWLM
                     SWIHEKLKVPLNRIDASRPITAYGLTSMKAIVLQQDFMEKFGVSFPPYLFFEKNSLKQ
                     LCEKALKVIEEA"
     gene            10227..>11277
                     /locus_tag="PKZ74_01530"
     CDS             10227..>11277
                     /locus_tag="PKZ74_01530"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPM91457.1"
                     /translation="MAIFTVPNIRIAGMAAAVPQKEVSNLDYRWISEKERQQVVKTIG
                     VEKRHIAEKGQTTSDLCFAAATRLLNDLKWNREDISALVFVSQSMDYIIPATSPIMQN
                     RLGLPKTCMAFDIGLGCSGFVYGLSVIASLMNQSGMKKTLLMAGDLSNVTSSYRDKST
                     YPLFGDAGTVTALEFDPSASPMIFNLQTDGAGHQAIMIRDGGVRNLMSKKSFTFKKHG
                     DGIYRSNLHISLNGIEVFNFSLREVVPNIKATLEYAGTTLADYDYYVFHQANRLINET
                     IRKMLRVEKEKVPYSIHKYGNTSSASVPLTMISELQEELKTQKLRFLLSAFGIGLSWG
                     TVLLETDSIVCSGVVT"
     assembly_gap    11278..11377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11378..12112
                     /locus_tag="PKZ74_01535"
     CDS             <11378..12112
                     /locus_tag="PKZ74_01535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM91458.1"
                     /translation="MTPFELTGKTILVTGASSGLGKQCAITASEQGATVIITGRNTER
                     LLETFEKLSGAGHQMFGADLTVQADISALVARLPELNGVVYSTGISDLAPARFVKKEE
                     IDRTFSISYNASVLLTGELLAKKKLAKGACSLVFISTISTKYPFVGGTMYISAKAALE
                     SYAKVLALELAPKGIRSNSISPAFVKTPMLDHTAEKYSDEAVRKIEAQQLLGLGEPED
                     VANVVVFFLSDASRWITASNLILGGG"
     gene            12244..12933
                     /locus_tag="PKZ74_01540"
     CDS             12244..12933
                     /locus_tag="PKZ74_01540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM91459.1"
                     /translation="MKLSKYGITLRRLREGDTEMVRQWRNSKQISQFMEYREQITPEM
                     QREWFKSVDDFDNFYYIIEYQGEDIGLINSSKIDWRDVSSEGGIFLWDEKYYETFVPV
                     WASLCLLETSFFILGASRSVIKTLKDNDRARKLNLHLGYELQPGQDEVYNQVYVMTAE
                     SFRRHAVKLIKAARLMAGHDPEDHRVFFDKEDIKSGLADFVYSKIDKEMVARSRESEE
                     GRLYIFRPTTV"
     gene            complement(12920..13993)
                     /locus_tag="PKZ74_01545"
     CDS             complement(12920..13993)
                     /locus_tag="PKZ74_01545"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM91460.1"
                     /translation="MDQRIEDQAIATLPVFFIIGRPRSGTTLLRLLFEAHPHVLIPPE
                     CPFIISLYKKYFKEIDWDEKLIREFSEDVYRQRYFDKWLVDKEVLYQNLLAGKGKTTF
                     REMVKRVELSYRSVYRKEELRMIGDKNPAYSLYPGRILRLFPDAKIIHITRDYRDNYL
                     SLINVNFEVPVVPLVIYRWKFAYRLMQKLKKQHPGQVYSMKYEDLATDPEKTFRDACA
                     FLELDFDPHVLSFYEKKEEVERSYAGSEEIAIVHKSLFNPISTDRMELWKKQMTPRQI
                     RVADLVAGKTAEKAGYGRQYPSFNIFLYLWILPTLVYASLMYRLIRMGDHLPYKWRNT
                     LNKRLGIFLKLYWKFNKRKVKPL"
     gene            complement(13978..15276)
                     /locus_tag="PKZ74_01550"
     CDS             complement(13978..15276)
                     /locus_tag="PKZ74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPM91461.1"
                     /translation="MSKRSLLRDVTGVVATRIFLITAGLLSSIVLSRNLGPENFGIYS
                     AILVLPLVVVSFAQLGMRASSIFHIGRKQYEPGEIAGSVVLILVMTSMLGILITALGY
                     LIMDEESFTGLYIILVLLSLPFRLAMAYFGGIFLGREQISRSNFINWFAEFLNLLAVL
                     VFVWLLGWGIKGALISLLASQATISTWALFVIRRDIGIRLKFHPEVLKSLLSMGVLFA
                     ASFGIIQLNYRIDILLLQKLSTMEEVGFYSLGVSIAEKLWQLPLAIGVVLMSRTANAD
                     DQQMINRISARLVRVALLAVFLASVVLFVISPWIVPAIWGEKFRQSITTVQFILPGII
                     FISIYRVLNSRLSGIGKPQISIYVFAPALILNILLNLWWIPLWGSFGAVMATNASYTL
                     GTLVYIFVYSKIVKMPVLQIFAYQKSDFDFLKRAKKWISG"
     gene            15367..16818
                     /locus_tag="PKZ74_01555"
     CDS             15367..16818
                     /locus_tag="PKZ74_01555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM91462.1"
                     /translation="MLFNSVDYLLFLPLVTALYFVIPVKWRWAFLLLASYFFYMCWKA
                     EYAILILFTTLVDYFVALKMDSLSTRRQKKPYLILSLAANLGMLAGFKYLNFFSESVN
                     FAMAGLDLPGRVPVLDILLPVGISFYIFQSLSYTVDVYRGTTKAERHAGKFALYVSFF
                     PQLVAGPIERSNNLLPQVNHPQAFSQVRLVSGLKLIAWGFFKKVVIADRLGVFVSYVY
                     ENPDEHKGITILLATVLFAFQLYGDFSGYTDIARGSARILGYDLMINFNRPMIARSLR
                     DFWNRWHISLTTWFRDYLLYSIPYVKNKKVMVSLMYRNLVITYLLMGLWHGAAWTFVI
                     FGLFHGILLVLENITENYRLRFFRWTGISRFPGVQTTLGMVATFILLVFSLFFFRAGS
                     IENSFILISNAFDFSTFKQTIGYIIRNNELIFGILMVIVLLTIEYFHEKYNLVKLVLS
                     RPTVIRWALYAGCVIFILFFGVLYKQKFIYFQF"
     gene            16825..17817
                     /locus_tag="PKZ74_01560"
     CDS             16825..17817
                     /locus_tag="PKZ74_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91463.1"
                     /translation="MTKKFTIRILLFLALLAGLNFVIDWAFKSFSAHRWVNLKMDEQF
                     GEYDGTLTWLAMGNSHNCVNTRILDNSFNYGSPGENYIRTYYKLKYILEKTGHKPEYL
                     LLQADISSFGPKISNRYEYNYYWIKYVDYLELARIKNDRDIINYWIEGKFFSYAGNYK
                     DFWLSIVYRIKMKEVNMFRGYREHRDYRNFADVPDRQKVAWDKAQMILTKNEPLDPAI
                     RVYFDMIMQLCREHQVKVLLIRFPEAKEFYEEQERLVPVGKLYEEVTAIAEKYMVFNG
                     ILDYHDMFFEHPEYFFDPDHLNVKGSDLFTEKLAEDLKKLTSFRPLEFSSHSGQ"
     gene            complement(17770..18819)
                     /locus_tag="PKZ74_01565"
     CDS             complement(17770..18819)
                     /locus_tag="PKZ74_01565"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM91464.1"
                     /translation="MENNSQLPFFFILGRPRTGTTLLRSLMDAHPNVQIPWECQFMIN
                     LYPKYGTLEHWNMETLEHFYSELLEQWQFKSWHIDHDKLRADLLACAGPTTYASVCRV
                     VYLNHISFYPKEEIRMIGDKNPGYSIYYDRLKKIFPDAKFIYILRDYRDNFHSVKNVD
                     FELPIVSVVVYKWKYFYKRALEAARKYPESFYLLRYEDLVSEPEPQLRGVCEFLGLPY
                     VPEVFDFYKMKSKAEETYPAVILKKHHKSLFNPVNTSRIGLWKQSMSQRQVRIADMVA
                     GKYADKAGYERDYRGFGILTGFQALPGVCYARFLYFMTGIIDRMPYRFRQKVLSKGPL
                     LLARMAAKFKRTKRS"
     gene            18913..20040
                     /gene="rffA"
                     /locus_tag="PKZ74_01570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             18913..20040
                     /gene="rffA"
                     /locus_tag="PKZ74_01570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HPM91465.1"
                     /translation="MEVVFNKPFITGRETHHLARIALSGKLSGNGAYTRICHDFFEKR
                     FGFRKTLLTTSCTDALEMAAILCEISPGDEVIMPSYTFVSSANAFILRGAVIRFADTG
                     PDYPNISPAEIRRLITEKTRAILVVHYGGVACEMDEIMEIARERNLLVVEDAAHAVDS
                     FYKGKPLGSIGHFGTFSFHETKNIICGEGGMLAINNERFVRRAEIIWEKGTDRAAFYR
                     GEVDKYGWKDIGSSFLPSEITAGMLYAQLEAFESIQNARKALWSKYYSALKPLHDTGK
                     VMLPEVPEYATINGNMFYILAGSPSERDALMDFLKSKGIQAVFHYLPLHSSDYYRDKH
                     DGSDLPNTRKFSGCIIRLPFFNEMKEEEVNYVVEEISRYFR"
     gene            20507..>21427
                     /locus_tag="PKZ74_01575"
     CDS             20507..>21427
                     /locus_tag="PKZ74_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91466.1"
                     /translation="MWALRSQLLGHSPHLSQEVLRAMADRTDVFPDEVLLEILASNPD
                     ELNRDTLLSYLEQKENPLPDYMISILQQAIEGITYKTILQENLAKYHAAKIQSAQDII
                     RDILYDSVFDSYNYRSWLGNLNSIVTDRQIISSYLSDNDTSSAIELLNLLPSLYGLEG
                     ESLTDYNNYKSLVEMQMSWIAQGKTLEDLDSLEVAEIEEIASDSINYAGYLARNILDY
                     ANIHHYCNCIASSDSTILKNFHHSSFVPESEKQLVKIYAEPNPAHTYVTFNYELLGEK
                     STGIVVISDLTGTMIHRFSVSGKIGQEVWNT"
BASE COUNT         5639 a         5083 c         5134 g         5471 t
ORIGIN      
        1 gcatgatcca tagaagctgt atcgatggtt gcggcatggc aatcgtactg ctgccagtcg
       61 tcaggataat tgttcagcac gatctggttg cgggctttca cgcgttcgaa cagtaccgtt
      121 ccgggcaaag gggtcatgat cgcgcattgg taagcatcca gtgagcttct caggatgtaa
      181 tccctccggg cgaataaatc ttccttttta tcgctctcca tgccgaagat cataaaagcc
      241 aggatcccga tcccgtgttt gtgggctttc cggaaggtct gttcatagga ttgaaccccc
      301 cttttcagat tcaggttttt tctgacatct ttcagggcct cttcattttc cgcttccacc
      361 ccgacaaaca ccatcataca accgcttttc ctggcccaat ggagaacttc ctcattttca
      421 gcgaaattta cagtagcctg gcacacccag aatttattca gtttccgttc gaccattccc
      481 cggaagaggc tgatggcgcg ttcgtgcgat cgttttgtat tctggataaa attatcatcc
      541 acaaaaaaaa taaggggacg ggttgtttcc tccagttcgt ccaggatgtc ctccacttta
      601 cgctcgcggt aggttttccc gcacaactgg gttacggaac agaattcgca tcccagcggg
      661 catccgcggg aagtgctgac caggtcgtaa acataaggat aggtgtagat atccctcctg
      721 acatgaggaa cagaatggat atcgacattt ccgccatcat acagtttttt tattttacct
      781 gcctcaaaat cagcaatcaa accctgccat gcgccttccg cttcgccggt aaaaacggta
      841 tcaacatatt cagcgacttc atccgtagcc agggaggcat ggatgccacc catgacggta
      901 tggattccct tttcccggta gacagaagct attttatagg cgcgcggcgc attggcggta
      961 aaaccggtaa aagcaaccag gtcagcgaat ctgaaagaaa atcgttcata actctcatcg
     1021 atcagttcca cttcccagtg attgggggta agggcggcta cgatgcccag gccgatcggc
     1081 ataaaacggg tactgtcgtc gtttgtgaaa ccctgtctta actcgtttgc cgggtttatc
     1141 aggagaagtt tcttgcgcac tttcagatgt aagatcgtga ataaccaaat ccaaaaatag
     1201 ggaaaaaggg actccaaccc tcccttttta cattaattgt tacttttgcg ccttatgatc
     1261 tccaggatat gatcgcgagc atccgaaaca ggaaaacact ggccgacctg ctgataagtg
     1321 cagccggtga atcgccggaa aacggggtcg gatttatccg ggccgacggc agcattcaat
     1381 tcctgacata ccccgggttg ctggcggaag cggaaacaat cgcctccgga atccggtcgg
     1441 aagggataaa agaaggtgaa tctgtcatga tcatcatcac acggaacgag gttttggtac
     1501 cgacgctctg ggggtgcatc ctggccggcg ttgttccgac gatcctccag ccgccggttt
     1561 cattcaccga agtcaatccg cccgcctcga aaatcgaaaa cgtttacagc atcctggaca
     1621 agcccgggat catcctgtcg gacgacctgg ccgggagttt caattcaacc gtcatctctc
     1681 ccgaaagcat tatcccggca agcgatctgg ctgccggcaa aaaccccgtc agacaggaat
     1741 tatcaaacga aacggagaac ctggcattta tccagttttc ttcgggaagc accggcgatc
     1801 ccaaagggat catgctgact caccggaata tccttgtaaa tattgcggcc atttgtgtcg
     1861 ggctcgatat tcacaaaagc gacgtcatgt gcaactggat gccgctatac cacgacatgg
     1921 gattgttcgg atttcacata tgcccggtat ttgcccagag caaccactac ctgatcgatc
     1981 cggtcgaatt catcaaaaaa cctaccttat ggcttgacgt tattgaacat tcggggagca
     2041 cgatcaccgg ttgtccgaat ttcggccagg ccttgttgct caggtatttg aaaaacaggc
     2101 aggaaagcgg acggaacctt tcttcattaa aagcaatagt gaacggtgcg gaacccatct
     2161 ccaaccggat catgtcggaa tttacaggca aactttcggt atccgggtta cgcaaagagg
     2221 tcatgatgcc ggcttatggc atggctgaga acacgctggc gatcaccttt gccgacctgt
     2281 ggagcgaacc gctcgtcatg aatttcaacc ggactttgct ccaggctcag gggatagccg
     2341 tgagggagga aaaaaaatca ggggaaacag tcgaactggt gagtgtcggt aaagcgctga
     2401 atgatgtgga aataaaaata gcggatgatg atggaaatgg gctggcagag gggatggaag
     2461 ggcacatccg gattcggggc aaaagcgtaa catcgggcta tatcaataat ccggaggcca
     2521 cggccgaagc gttcgaagac ggatggctga aaacaggcga caagggattt ttctatgacg
     2581 gacagctcta tatcaccggc aggatcaagg atatcatttt tgtgcacggc cagaacctgt
     2641 atgcccatga cctggagaac ctggcctcga aacatgccga catcccctac ggtaaagtca
     2701 tcatcggcgg ctctttcgat ccgaaaaaag gaaaagacca ggtgatcctg ttcctggtcg
     2761 gatcggcaaa ccaggccctt tgtgatctgt tcctggaact gaaaggattt ttcagggaca
     2821 cctacggagt acatattgat gtatttgtgc cggtgcggtc gaaccaggtg ccgaaaacaa
     2881 gcagcgggaa aattcaaagg tataagctgc tggaagatta ccgcgacggg gtttttgatg
     2941 aggcgatcgc agaaatcagg aggttgatga ggtaagggcc tcagcacgat tttggtcctt
     3001 cgcttcgcta ccaatgacaa gttagtaaat ttctactgaa ccgctctccg gaggagagcc
     3061 ctatttgtag caaaaaggac gggaaagaac aaaagcccct ccctttggaa atattggatt
     3121 atagcgaaaa accttcacag ggaggggttg gggagggaaa ccgaaaatgc atctttcccc
     3181 tccccttaat cccctcccat gaagatatta gcttgaaaaa ccggattatc gcgggagggg
     3241 aaaattgtgc gggtgccatc gcctttgcta caaatgtgga actcctccgg agtaatgaaa
     3301 agctaatatc atcccatatt gtggagtttg ttaaagtctt taaactcggg atgacaattg
     3361 cctcatcgct ttttaccctg gcatacattc gccgtcattc aggcttaagc cggtatccaa
     3421 atgttagtcg actgtttcag tgaaattgtc attcatatca atttgatcat gaaatctgcg
     3481 agaaataaaa tacctttgcc gcttcaaatt tattagaaat gactatagaa gattttattg
     3541 ccaggattga agaggagttt gacgacctgg aacccggcaa gctcaggccg gaatcgaatt
     3601 tcagggaggt gtttgagtgg aactcgatca acgcattaat ccttatcgcc ctggtaaaaa
     3661 ccaattacga tgtggccatc aatgccgaag acatcaccaa atccaaaacg gtgaatgatc
     3721 tgtataagat cgtaaaggaa cgaatagctt cataaagatt ttcgcactgc gacgactaaa
     3781 gccgtcggtt ataaggtttt cttccgacga ctaaagccgt cggttccgat aaggggtttc
     3841 tgattccttt atcaaccgga aagctctttc tgacagttca ttcagcgata ttctttcaaa
     3901 aaacaggtaa ggaggaaagt tcacctggaa attctccagg aaatcctgct ggagttgtac
     3961 ggctttcatg gaattcaacc cgtaaacggt tataggtttg gataaatcaa tcttctccag
     4021 cctgatcttt aatttgagat ggatccatgt catcagccat gcacggatag acaccaggtc
     4081 gggtggtgat agagattcct cattatcttc tttataatca gtcagaaagg actctccgac
     4141 aacttccagg gagtggctta agaaagcctt ctgacaagct tttcgctgaa tttttccact
     4201 tgatgttttc ataatggtag ctgtacggat aagctgaata ccatagacgg ccagttccat
     4261 ttcagcggca atttttgtcc ggatggcatt gacaacctcc tccacgttca gatccttcat
     4321 cgctgtgcgt tccacctcag cgataattat caatctttct gtatcatcaa ttataaccga
     4381 aaatgccgcg ctggaatttg ctctgatagc tttatggctg ctttcggtaa gatattcaat
     4441 atcctgaggg taataattac tcccccggat tatgattaaa tcttttaatc tgccggagat
     4501 aaagagcttt ccttcgtgaa aaaagccaag gtcgccggtt ctgagaaatg gccctctgcc
     4561 gttgctggct gtatatgcat taaaaatgtt ccgggtttcc tcatcatttt cccaatatcc
     4621 ttttgtgata atcgagcccg atacccaaag ctcacccact acgtcgccgg ggcagggttc
     4681 caatgtgtca ggattaacaa tggtgatgga agtatcaatc cagggatttc ctacggaaac
     4741 gaggctatag gcattttcat tttcttcggc aacaatacgg atccggtttt tttcaagttc
     4801 ggtcttatct acataaacta caacgggttt atcgcacacc ttgcctcccg agagtatgag
     4861 cgttgcctct gccattccat acgtcggata aaacatctcc tcacggaatc caaacggacc
     4921 gaattttccg gtaaatgctt tcatggtgga ggctcttacc ggttcagccc cgttatacag
     4981 tgattttaga ctactcaggt ccaggtcttt gcattcgtct tccgaaatat ccgcaacaca
     5041 aagatcgtat gcgaaattag gcgcgccggc cagtgttgct ttatacctgg taatggcttt
     5101 cagccatctc gacggtttct ggatgaatga aaccggcggc ataagaatgc cagtatatcc
     5161 tgtgtaaatg gattccagga caccaaaaac caggcccata tcgtgaaaaa ccggaagcca
     5221 gtgtacggaa atagaatccg gtgtaagctc aacagcttgt ctcagaaatt caaggttcct
     5281 catcaggtta tgatgggtca ccataacgcc tttgggtcta cctgtagagc cggaggtgta
     5341 ctggagcagg gcgatttggg gatttggaga tttggagatt tggagattgg aggatttgga
     5401 gatttggaga ttgggggatt ggatttgtga ttttgagatg ggatgggttg agagccaggt
     5461 caatttttga agttcgatgg tgtctgaaaa tgatcgttag ctggtttgcc tgatgtcgtc
     5521 cgttgaaagc acaatgctgg cgccggaatc gcttgccagg gccctgagcc gctcaagcga
     5581 acggtttttc ctgggagggt atgccgggac tgcaatgact cctgcgtaaa aacaaccaaa
     5641 gagagctttg ataaattcaa gcccggcagg atagaacata agggcgcggt cgccgggccg
     5701 ggtggcttca gttaattccc gggaaatggc taacgctgaa ttataaagct gtccgtatgt
     5761 gatcctttct tcatcgttct ccccgtcgcg taaaaatata aacgcacacc ggtcaggtgt
     5821 gcaggcagcc ctcttattca ataggtcagc gagcgttaac aggcctgtgc cttcatctga
     5881 agcctgttca atagtaattt cagagcgtct catgaggtaa agaaaacaaa tatactataa
     5941 aaacttttat agttcttcta gttttcttta ataattttta tggctttctc acaaagttct
     6001 cttaatgaag atttttcaaa aaacagataa gggggaaaac tgacgctata tctttccagg
     6061 aagtcctgct gcagggccac tgctttcatc gaggtaagtc cataagcggt tataggcctt
     6121 gccaggtcga tacggttaag gggaattttc agtttttcat ggatccaggt catcagccag
     6181 gcttccagat ttacaatatc gaccgatgtt ggttcgggct gatcttcatg gaactgcgtc
     6241 tcaagaacgg attccccgag tgaatccagt ttattttgca ggaatgcctc ccggcacgct
     6301 ttgcgctgaa ttttaccact ggaagttttc aggatggaag ccgttcgaag caactggata
     6361 gcatgcacgg aaagttccgt ttccatgacg atattctccc ggatcctgtc gcaaacccca
     6421 tccacatcca ggtcacgtat tgctgtgcgt tccacttcag ctacaacgac cagcttttct
     6481 tcaccgctga tatccacagg aaatgcagcg ctggcatttt tcctgatcga cggatgtgaa
     6541 ttttcggtaa ggaattcgat atcctgaggg taaatatttc gtccattaat aatgatgagg
     6601 tctttatatc ttccggagat aaatagctca ccatcatgaa aaaagccaag gtcaccggtc
     6661 cgcagatatt tttcacccgg atcaccggct atttcagcag caaaaacttc tttcgatgcg
     6721 tcaggttttc cccaataccc ggctgtaacg atagagccgg aaacccagat ttcgcccacc
     6781 tcatcgtcgt tcaataaccg gcaggtctcc ggatcgacaa ttttcacccg tgtatcaatc
     6841 cagggataac caacgccgac gaggtgcctg gcatcaggat gctgatcact gacaggaacg
     6901 atcctgtttt tttcaagttc gccggccgaa aggcaaagat aaaccggtcc tcttccggca
     6961 tgcgggccgg tgataatgag ggtggtttca gccattccgt agcaaggata aagtttttcg
     7021 tgtgaaaaac catattcccg gaaggatgaa acaaaccgtt cgaaggtcga tttacggata
     7081 ggttctgcac cgcagtacag cgtttccata gatgaaaggt ccaggcttat tttttcttct
     7141 tcagcgatgc cctctgcgca aagatcgaag gcgaaattcg gaccgccgcc gtgtgtgccc
     7201 ctgtactttg tgatcgcttt cagccagcga acgggtttct gcagaaaact aacgggcggc
     7261 agaattacgc cggggaaacc cgtataaacc ggaaccagta cgccatctat caatcccatg
     7321 tcatgaaaac tgggaagcca ggtaacggaa acgcttttcg gcgaaagcgc aaaactttgc
     7381 cggatatatt cggcattccg gataatattc cggtgggtga ccatgactcc tttgggatga
     7441 ccggtcgagc cggaggtgta ctgaagcagg gcgatgtctg gatttgggga tttggagatt
     7501 tggagattgg agatttggag attggaggat tgggggattg ggggattggg ggtgatccat
     7561 ggtatcccct gaagttcagt tacatcagcg aaagagcgtt cggctgattg gcggatatcg
     7621 tcggttgaga ggacgaggct tgaaccggag tcattcacca ggagacgaag ccgttcaagc
     7681 gaccggttct tccgtggagg ataggccggt acggcgatca cgccggcgta aaagcatccg
     7741 aacaatgctt tgataaaatc aaggccggga ggaaaaagca tcagggcccg atcgcccggt
     7801 tttgctattt caagcagaga atgagataaa tcaattgctg cctggtgcag ctcccggtat
     7861 gtaatatgtt cctccgattc atcaccatcc tgaataaaaa taaatgcggt ctggtcagga
     7921 tttgaaacag ccctgccttg caggatatgc atcagcgaag gatccttgat aacccctgca
     7981 ggtaagaact catcgttaaa aaaattgtct tccatttttc cggtgccgct aaagtactaa
     8041 aaaatcagcg catcggttca ggcttcttct ataaccttca gcgctttttc acagagttgc
     8101 ttcagcgaat tcttctcaaa gaacagatag ggaggaaaac tgaccccaaa tttttccatg
     8161 aaatcctgtt gaagaacgat tgctttcatg gaggtcaatc cataggctgt aatgggccgg
     8221 gaggcatcga tccggttcag cggcaccttg agcttttcat gtatccagct cataagccat
     8281 gcttccaacc tgacaatgtc gacggtagta tcatccggtt catcggtatg atattgagac
     8341 tcaagataag attctccaac cacgtcaagg gttttgttaa gaaatgcttc catgcaggcc
     8401 ctgcgctgga ttttaccact ggaagtcttc agaatggaag ccgttctgag caactggatg
     8461 gcatgaactg caagctccgt ttccagggcg acattttccc ggatgctgtc acatactgca
     8521 tcgacatcca gccctatgat agcggaacgc tctacctcgg ccacgatgac cagtttttct
     8581 tcaccattaa cctcgacaga aaatgctgcg ctggaatttt ggcggagggc cgggtgggaa
     8641 ttctccgcaa tgaactcaat atcctgggga tagatattcc ttccgtagat gatgatcata
     8701 tctttcaacc ggcctgatac ataaagttct ccttcatgca aaaagcccag gtcaccgctt
     8761 cgcaggtaat tcgtacccgg atcatcatcg agcctggcct ggaaaacttc ccggttgatc
     8821 tcagctttgt tccaatatcc cggcgatacg ctggagccgc ttatccagat ctcacccact
     8881 tcatccgcca tacagggtgt aagggtttcc gggtctacaa tccggatttt catgtcgagc
     8941 cagggaaaac ccacgcttac caggcatttt acttcgcttt cactattccg ggggagcctg
     9001 actttattct ttttcaatga ctcgccgtct acagcaaggt atttaggcct tctgccagcc
     9061 ggaggccctg tggttatgag cgtagtctcg gccatcccat agcagggata aagcatctct
     9121 tccctcaaac cgtgagcctt gtaagcattg aaaaaattat cgaacgtcga ttttcggatg
     9181 ggttcagcgc cacaatagat tgttttgagc gaagaaagat ccagttcctg cttttccccg
     9241 gctgtgacgc tctctgtcag caggtcgtaa gcaaaattag gtgcaccgcc catcgtgcca
     9301 ctgtactttg taatggcccg aagccatctg acaggcttct ggaaaaatga aacgggcgac
     9361 atcagcacac tgggaaagcc ggtgtaaaca ggttcaaaaa tctggccgac aagtcccata
     9421 tcgtgaaacg tagggagcca cgaaacagct actgttttcc gggagaaccc gaagctgttc
     9481 cggatgaatt cgcagttgcg cataatgttc cggtgggtaa tcatcactcc tttaggatta
     9541 ccggttgaac cggaagtgta ttgaaggaga gcgaccgaat cgggatcagg gaaagaagac
     9601 gaatgagggt caacctgacg tgttttcttc ggaatgtagg attcgagatt ctcatgaagg
     9661 tcgggattat ctctaaaaac gatccattcc aggctgcgta gttccgttaa atctgaaaag
     9721 gaccggttga acgactgata aatctcggtg gtggtaatta cagtggtagc ttcggagtcc
     9781 gttacaatct tcatgatccg gtcaagcgaa cggttttttc tcgggggata agccggaaaa
     9841 gcaataaccg aagcataaaa acaagccata agagatttga tgaattccag cccgggagga
     9901 aaaagcagta atgcccgttt gccggccaca ttcatttcca tcatccgccg ggccagcccg
     9961 gatgctgagc tccccaattc ccggtaggtg atcctctctt catcactctc cccgtctttc
    10021 aggtaaataa atgcgatctc gtccggcgta ttactgacgc ggtaattcag cacatccagc
    10081 aaagaaaaag tattggtatc agcttcggtg atacggtcat aaaggaagtt ctcaaggccc
    10141 atagataaaa cccggcttaa ttaaaaatcc ttacaaaatt agaaaaggaa tcgtaacata
    10201 tctaattaaa attaactttg tctgatatgg caatcttcac cgtaccaaat atccgcatag
    10261 ccggcatggc agccgccgtt ccgcagaaag aggtttccaa ccttgactac cgctggatct
    10321 ccgaaaaaga aaggcagcag gtggttaaaa ccattggcgt cgaaaaaagg cacattgcgg
    10381 aaaaggggca gacaacatcg gatctttgct ttgcagccgc tacccggttg ctgaacgacc
    10441 tgaaatggaa ccgcgaagat atctcagcgc tcgtattcgt ttcccaatcg atggattaca
    10501 ttatcccggc cacatctccc atcatgcaga acaggctggg gcttcccaaa acctgcatgg
    10561 cattcgatat cggcctgggc tgttcggggt ttgtttacgg attgtctgtc atcgccagcc
    10621 tgatgaacca gtcggggatg aaaaaaacgc tcctgatggc cggcgatcta tcgaatgtga
    10681 cctcatctta tcgcgacaaa tcgacctacc cgttatttgg tgatgccggg acggtgacag
    10741 cgctggaatt cgatccatcc gcttcaccga tgatcttcaa cctccagacc gacggcgccg
    10801 ggcaccaggc cattatgatc cgcgacggag gggtaagaaa cctgatgagt aaaaaatcat
    10861 tcacctttaa aaaacacgga gatggaattt accggtcgaa cctgcatatt tcactcaatg
    10921 gtatagaggt attcaatttt tcgctgcgcg aagtggtgcc caatatcaaa gctacattgg
    10981 aatatgccgg gacaaccctg gcggattacg attattacgt cttccaccag gccaaccggc
    11041 tgatcaacga aacgatccgc aagatgctcc gggtggagaa agagaaggtc ccctactcca
    11101 tccataaata cggcaatacc agcagcgctt cggtcccgct gacgatgata tcggaactgc
    11161 aggaagaact caagacacag aagctcaggt tcctgctctc tgccttcggc atcggccttt
    11221 cgtgggggac ggtgcttttg gaaacggatt cgatcgtatg cagcggcgtg gtgacagnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg accccattcg aactaaccgg
    11401 caaaacgatt ttagtcacag gagcttcctc agggttgggg aaacagtgtg ccattacggc
    11461 aagtgagcag ggcgcaacgg taatcataac cgggcgcaat accgaacgac ttctggagac
    11521 atttgaaaag ctctccggcg ccgggcatca gatgttcggt gccgacctga ctgttcaggc
    11581 cgatatttcg gctcttgtag cccgactccc ggagctcaac ggggtcgtat acagcaccgg
    11641 catctccgac ctggcgccgg cccgtttcgt gaaaaaagaa gagatcgaca gaaccttctc
    11701 catcagttat aatgcctccg tcttactgac cggcgagctg ctggcaaaga aaaaactggc
    11761 gaaaggggcc tgttcgctgg tcttcatctc caccatctcc acgaagtacc cctttgtcgg
    11821 cggtacgatg tacatcagcg ctaaagcggc cctggaatcc tatgcaaagg tgcttgcgct
    11881 ggagctggcg cccaaaggga tccgttccaa cagcatttcg ccggcctttg tcaaaacccc
    11941 tatgctcgac catacggctg aaaagtattc ggatgaagcg gtccgtaaga tcgaggcgca
    12001 gcaattgctg ggcctgggcg aaccggaaga tgttgcgaat gttgtggtat tcttcctgtc
    12061 ggatgcttcc cgctggatca cagcgagtaa tttgatttta ggcgggggat aaaagcagtt
    12121 caaagttcaa agttcaaagt tcaaagtttt aaggtcaaac ttattgatta ccagtattat
    12181 tctttgtgtt cctttgtgaa accctttgtg cgcctttgtg gttaaaaaaa tattcaattc
    12241 aacatgaaac tctcaaaata cggtattacc ttaagacgtt tgcgggaagg cgacaccgag
    12301 atggttcggc aatggcggaa ctcaaagcag atcagccagt tcatggaata ccgggaacag
    12361 atcacacccg aaatgcagcg ggaatggttt aaatcggtag atgatttcga caatttttac
    12421 tatatcattg aataccaggg agaagatatt ggcctgatca attcaagcaa gatcgattgg
    12481 cgtgatgtga gctccgaagg gggcattttt ctctgggatg agaaatatta tgagactttc
    12541 gtcccggtct gggcctcgct ctgcctcctg gaaacatcat tttttattct aggcgcttcc
    12601 cggtcggtca ttaaaacgtt gaaggacaat gatcgggcca ggaaactgaa cctgcacctt
    12661 ggatatgaac tgcaacccgg ccaggatgaa gtttacaacc aggtgtatgt aatgacggcc
    12721 gaaagtttca ggcgccatgc cgttaagctg ataaaagcag caaggctgat ggctggccac
    12781 gatccggaag accaccgcgt ttttttcgat aaggaagaca ttaaatcggg cctggccgac
    12841 tttgtttata gcaagatcga taaggaaatg gttgctagat cccgtgaatc cgaagaaggc
    12901 aggttgtata tcttcaggcc tacaacggtt tgactttccg cttgttaaac ttccagtaaa
    12961 gcttaaggaa aataccaagt cttttattca gcgtattcct ccatttgtaa ggaagatgat
    13021 ctcccatccg gatcagccgg tacatcaatg aggcataaac cagtgtcggg agtatccaca
    13081 ggtatagaaa tatattgaag gaaggatatt gccgcccgta gccggctttt tccgccgttt
    13141 tgccggcaac caggtcggcg acccggatct ggcgtggcgt catctgcttt ttccagagct
    13201 ccatccggtc ggtggaaata gggttgaaca aacttttatg cacaatggct atttcttccg
    13261 aaccggcata agacctttcg acttcctctt ttttctcata aaaactgaga acatgcggat
    13321 caaaatcaag ctccaggaac gcacaggcat ccctgaacgt cttttcagga tcagtggcca
    13381 gatcttcgta tttcatcgaa taaacctgcc ccggatgctg tttcttcaat ttttgcatca
    13441 gcctgtaagc aaacttccac cggtatatca ccagcgggac aacaggcact tcaaaattca
    13501 cattgatcag cgaaaggtaa ttgtccctgt aatctcgggt gatgtgaatg attttcgcat
    13561 cggggaagag cctgaggatc cggccgggat ataacgaata cgccggattt ttatccccga
    13621 tcattctgag ttcttcctta cggtaaaccg aacggtaact taattccacc cgttttacca
    13681 tttcccggaa agtagtcttc ccttttcccg caagcagatt ctgataaagt acttctttgt
    13741 cgaccaacca tttatcaaaa tagcgttgcc gataaacatc ttctgaaaat tcccggatca
    13801 gtttttcatc ccagtcaatt tctttaaaat attttttata aaggctgatg ataaaaggac
    13861 attcgggagg gatcagcacg tggggatgcg cttcaaaaag aagccggagc aacgtggttc
    13921 cggaacgggg ccttccgata ataaagaaga ccggcaaggt ggcaatggct tgatcttcta
    13981 tccgctgatc cattttttcg ctctcttaag gaagtcaaaa tcacttttct ggtatgcaaa
    14041 tatctgcaaa acaggcatct ttacaatttt tgagtacaca aaaatataaa ccagcgttcc
    14101 cagggtatag ctggcattgg tagccataac cgcgccgaat gaaccccaca gcgggatcca
    14161 ccagaggtta agcagaatgt tcaggatgag tgccggggca aatacataga ttgaaatctg
    14221 cggtttcccg attcccgaga ggcggctgtt cagcacccgg taaatgctga tgaaaatgat
    14281 ccccgggaga ataaactgaa ctgtggtgat actctgccgg aacttttccc cccaaatcgc
    14341 cggcacgatc cagggagata ttacaaagag cacgacagag gcgaggaaaa ccgcaagcaa
    14401 agccaccctg acgagccggg cactgatcct gttgatcatc tgctgatcgt cggcattggc
    14461 cgtccggctc atcagcacca cgccgatagc cagcggcagt tgccatagct tctcggcgat
    14521 cgaaacgccc agcgaataaa atccaacttc ctccatcgta gagagtttct gcaacagcaa
    14581 aatatcgatc cggtaattca actggatgat accgaaggat gcggcaaaca gcacgcccat
    14641 cgacaataag cttttcaaca cttcgggatg gaattttaac cggattccga tgtctctcct
    14701 gatcacaaaa agtgcccagg ttgaaatagt agcctgtgag gccagcaggc ttatcagggc
    14761 gccttttatg ccccagccca gaagccagac aaaaaccagg actgccagca gattcaggaa
    14821 ttcggcgaac cagttgatga aatttgaccg gctgatttgc tcacgaccga ggaatatccc
    14881 gccgaaataa gccatagcca gccggaaagg cagggagagc agtacgagga tgatgtaaag
    14941 tcctgtaaac gattcttcat ccatgatcag gtaaccaagg gcggttatca gtatgcccag
    15001 catggatgtc ataaccagta tcaaaaccac actgccggct atttcaccgg gttcatactg
    15061 tttacgccct atatgaaaaa tggaagaggc cctcatgccc agttgggcaa agctgacgac
    15121 aaccagcggc aagaccagga tggccgaata aatcccaaaa ttttccgggc ccagattcct
    15181 tgaaaggact atgcttgaca gcagccctgc cgtgatcagg aagatccttg tggccactac
    15241 cccggtgacg tcccgcagaa ggcttcgttt tgacatggcg caaaggtaat ttttttgaag
    15301 ctatgtttcc cgggctgaat gccttgatga aaatacttac atttgcggcc taatattcag
    15361 ttgctcatgc tgtttaactc ggtcgattac ctcctgttcc tacccctggt tacggcattg
    15421 tactttgtta ttccggtcaa atggcgctgg gcctttttac tgcttgccag ttactttttc
    15481 tacatgtgct ggaaagcgga atatgctatc cttattctct tcactaccct ggtcgactat
    15541 tttgtcgccc tgaaaatgga ttctctttca acaaggcggc agaaaaaacc ctatctcatc
    15601 ctgagcctgg ccgccaacct gggcatgctg gccggtttca agtacctgaa cttcttttcg
    15661 gaaagcgtga attttgcgat ggcgggtttg gacctgccgg gcagggtacc cgtactggac
    15721 attctgctgc cggttggtat ctctttttac atctttcaat cgctcagtta tacggtcgat
    15781 gtttaccggg ggacgacgaa agccgagagg catgcaggga aattcgccct gtatgtttcc
    15841 tttttcccgc aattggtggc aggacctatc gaacgatcca acaatctcct cccccaggtc
    15901 aaccacccgc aggctttcag ccaggtgcgg ctggtcagcg gattgaagct catagcctgg
    15961 ggattcttta aaaaagtggt catcgccgac cggctggggg tatttgtcag ttatgtctat
    16021 gaaaatccgg acgaacacaa aggaattacg attctcctgg ctacggtcct ttttgcattt
    16081 caactttacg gagatttttc aggatatacc gacatagcgc gcggttcggc ccgtatcctc
    16141 ggttatgacc tgatgatcaa cttcaacagg ccgatgatcg cccgatcgct cagggacttc
    16201 tggaaccgct ggcatatatc gctcactacc tggttccggg attatctcct gtactccata
    16261 ccgtatgtga aaaacaaaaa ggtcatggtt tcgctgatgt accgaaacct ggtcatcacc
    16321 tacctgctta tgggattatg gcatggcgcc gcatggactt tcgtgatctt cggcctgttt
    16381 cacggaatcc tgctggtgct ggagaatatt accgaaaatt accggctgag gttcttccgc
    16441 tggacaggaa tttcccggtt tccgggggtg cagacaaccc tcggaatggt cgctacgttc
    16501 atcctgctcg tcttttccct gtttttcttc cgtgccggca gcatcgaaaa cagcttcata
    16561 ctgatttcaa atgcatttga cttcagcacc ttcaaacaaa ccatcggtta catcatcagg
    16621 aacaatgaat tgatttttgg gatattgatg gtaatcgtct tattaaccat tgaatatttt
    16681 catgaaaaat acaacctggt aaagctggtg ctttccaggc caacagtcat acgttgggca
    16741 ttgtatgcag gatgcgtgat tttcatcctc ttcttcggtg tgctttacaa acaaaaattc
    16801 atctatttcc agttctgata aggcatgacg aaaaaattca cgatacggat cctgttgttc
    16861 ctcgcccttc tggcaggcct gaacttcgtc atcgattggg ctttcaaatc gttttccgcg
    16921 cacagatggg tcaatctgaa gatggatgag cagttcgggg aatacgacgg aacactgacg
    16981 tggcttgcca tggggaattc gcataactgc gtcaacacgc gtatcctcga caacagtttt
    17041 aattacggtt ctccggggga gaattacatc cggacttatt acaagctgaa atatatcctc
    17101 gaaaagacag gccataagcc ggaatatctt ttgctccagg ccgatatttc cagtttcgga
    17161 ccaaaaatat ccaaccgtta tgaatacaat tactactgga taaagtatgt ggattatctg
    17221 gagttggcac ggatcaaaaa cgacagggat atcatcaatt actggattga agggaagttt
    17281 ttttcgtacg ccggcaacta caaggatttc tggttatcga tcgtgtaccg gatcaaaatg
    17341 aaggaggtaa acatgtttcg cggataccgt gaacacaggg attaccggaa ttttgccgat
    17401 gtgcctgatc gtcagaaggt agcctgggac aaggcgcaaa tgatcctgac caaaaatgag
    17461 cctttagatc cggccatccg ggtatatttt gatatgatca tgcagttgtg ccgggagcac
    17521 caggtgaaag tgctgctgat ccgttttccc gaagcaaaag agttttacga agaacaagaa
    17581 agattggtac cggtcggaaa attgtatgag gaggttaccg caatagctga aaagtatatg
    17641 gttttcaatg ggattctgga ttatcacgat atgttttttg aacatccgga gtactttttt
    17701 gacccggacc atctcaatgt taaaggatcg gaccttttta ccgaaaaact ggccgaagac
    17761 cttaaaaagt taacttcttt tcgtcctctt gaatttagca gccattctgg ccagtagcaa
    17821 aggccctttg ctcagcacct tttgcctgaa gcggtaaggc atacggtcga tgatccctgt
    17881 catgaaatag aggaaccggg catagcatac cccgggcagg gcctgaaaac ctgtcagtat
    17941 gccgaaccca cggtaatctc tttcataacc cgctttatcc gcatattttc ccgctaccat
    18001 gtcggcgatc ctcacctgcc gctggctcat cgattgtttc caaagcccta tcctgctggt
    18061 attgaccgga ttaaaaagac ttttatgatg ctttttcagg atgaccgcag ggtatgtttc
    18121 ttcggctttg cttttcatct tataaaaatc gaagacctcg ggaacatacg gtaagccaag
    18181 aaattcacaa accccgcgca gttgcggctc cggctcgctg accagatctt catagcgaag
    18241 caggtagaaa gattccgggt attttcgggc tgcttccagg gctcttttgt aaaaatattt
    18301 ccatttatag acaaccaccg atacgatagg gagttcgaaa tcgacatttt tcaccgaatg
    18361 aaaattatct ctgtaatccc ggagaatata aatgaatttc gcatccggga agatcttctt
    18421 caaccggtcg tagtaaatcg aataaccggg atttttatcc cctatcatcc tgatctcttc
    18481 tttagggtag aaggagatgt ggttcagata aacaacccgg caaacagatg cgtaggtggt
    18541 gggccctgca caggccagca gatcggccct aagcttatca tggtcgatat gccaggattt
    18601 gaattgccat tgttccagca gttcggagta aaaatgttcc agtgtttcca tgttccagtg
    18661 ttccagggtg ccgtactttg ggtataagtt gatcatgaac tggcattccc acgggatctg
    18721 gacattggga tgggcgtcca tcagcgaccg gagcagcgtg gtacccgtcc ggggccggcc
    18781 caggataaag aaaaacggaa gctgagaatt attttccatc aataaactaa aacaggcaaa
    18841 agtaacccct tttcgcaaat gctgcgcaaa tatgcttatt ttcgcaacac cttaatcctt
    18901 aagttgattt cgatggaagt tgttttcaat aagccgttta tcacggggcg cgaaacgcat
    18961 catctcgccc ggatagcgct gtcgggaaaa ttatcgggaa atggggccta tacaaggata
    19021 tgccatgatt ttttcgaaaa acggttcggt ttccggaaaa cacttttgac cacttcctgc
    19081 acggatgccc tggaaatggc cgctattctt tgtgagatca gccccggcga cgaggtgatc
    19141 atgccgtcgt acaccttcgt gtcgtcggcc aacgcgttca ttctcagggg cgccgttatc
    19201 cgttttgccg ataccggccc cgattatccc aacatcagcc ctgctgaaat ccggcggctg
    19261 atcacggaga agacccgggc catcctggtg gtgcattacg gtggtgtagc ctgcgaaatg
    19321 gacgagatca tggagatcgc ccgggagcgc aacctgctgg tcgtggagga tgccgctcat
    19381 gcggtcgaca gcttttacaa aggcaaaccg ctcggcagta tcggtcattt cggcactttt
    19441 tccttccatg aaaccaaaaa catcatttgc ggagagggag gcatgctggc catcaacaac
    19501 gagcggttcg tccgccgggc cgagatcatc tgggaaaagg ggaccgaccg ggctgccttt
    19561 taccgcggtg aagtcgacaa atacggatgg aaagacatcg ggtcgtcttt tctgcccagt
    19621 gaaatcacgg ccggcatgct ctatgcccag ctcgaagcgt tcgaatccat tcaaaacgcc
    19681 cggaaagcgc tctggtcgaa gtattattcg gctttaaaac cgctgcacga tacaggaaag
    19741 gtcatgctgc cggaggtccc ggaatacgct acgattaacg gcaatatgtt ttatatcctg
    19801 gccggcagtc cttcggagag ggatgccctg atggatttcc ttaaaagcaa agggatccag
    19861 gccgttttcc attacctccc gctgcattcg agtgattatt accgggataa acacgatggc
    19921 agcgacctcc cgaatacccg caaattttcc ggttgtatta tccgccttcc gttttttaat
    19981 gagatgaaag aggaggaggt gaattatgtg gtggaagaga taagcaggta tttcagataa
    20041 ttttgtttct tgggattata ttggcagtcc aacgactaaa gccgttggtt ccaaaaatga
    20101 tagaaccggc tgattttatt ataaatcctc aaatcaccaa atcctaaatc ctcgagactc
    20161 catctgcccg aaatgagatg gagtctccta gcaatcgaga tgccatctga aatttgagat
    20221 ggagtctcgc aagattgaga tgccatctta ctaaaaccag atgacatctc aaaccaaaaa
    20281 gttaacaaac gcacttgact ttcttatctg attgaactat atttgttcca tccgtttcat
    20341 tcattcattt accaatcaat caatccaaat ggaatggcgt gttcaaataa gttatcccta
    20401 tcaataggga gagggtaata taatgatacc cttatacgaa tcgctaattg atggaggaaa
    20461 tacgcaaagt gagctatccg atattgaatc tgcagaatct gatgatatgt gggctttaag
    20521 aagtcagcta ttaggtcatt ctcctcatct ttcccaggag gtattgcggg caatggccga
    20581 ccgtaccgat gtttttcctg acgaggtttt acttgagatc ctggcctcca atccggatga
    20641 attgaaccgg gacaccctgc tatcctatct tgaacagaag gaaaatccgc tgccggatta
    20701 tatgatctcc attttacaac aagccattga aggcattact tataaaacta ttctgcagga
    20761 aaacctggca aagtatcacg ccgctaaaat tcaatccgca caggacataa tacgggatat
    20821 tctttatgat tctgttttcg atagctataa ttaccgaagc tggcttggaa atctgaattc
    20881 cattgtaacc gacagacaaa tcatttcatc gtacctttcc gataatgata cctcaagcgc
    20941 aatagaactg ctgaatttat taccttcgct ctacgggctt gaaggagaaa gtttgacgga
    21001 ttataataat tataaatcat tggttgaaat gcagatgtcc tggattgcac aagggaaaac
    21061 tcttgaagat ctcgacagtc ttgaggtggc tgagatcgaa gagatcgcat cagacagcat
    21121 aaactatgct ggatacctgg ccagaaacat tctcgattat gccaatattc atcattattg
    21181 caattgtata gcttccagcg acagtacgat cctgaaaaac tttcatcatt ccagcttcgt
    21241 tcctgaatcg gaaaaacaac tggtcaagat ctatgccgaa ccaaatcccg cgcacactta
    21301 tgtcacattt aactatgagc ttctgggaga aaaatcaaca gggatcgtgg ttatttcaga
    21361 tttaactgga actatgattc accggttcag cgtaagtgga aaaatcgggc aggaggtgtg
    21421 gaataca
//
LOCUS       DAOPNH010000013        21189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2341_length_21189_cov_6.048973, whole genome shotgun sequence.
ACCESSION   DAOPNH010000013 DAOPNH010000000
VERSION     DAOPNH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_21189_cov_6.048973"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="PKZ74_01580"
     CDS             <1..2145
                     /locus_tag="PKZ74_01580"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPM91467.1"
                     /translation="TGSNGKTIVKEWLYQLLSKSRKTVRSPKSYNSQIGVPLSVWQMD
                     TPYEMAIFEAGISEPDEMIRLETIIQPTIGVFTNIGEAHGENFMSLQQKVGEKLFLFT
                     HVETLIYCLDHPEVREVIIRSGISKNIKTFTWSHKQEADLRIREVRPENAHKTNIRAV
                     LRGREISITIPFSDNASVENAIHCWSVMLLLGYEAESLTGLFEALQPIAMRLELKAGI
                     NHCTVINDSYNSDINSLSIAIDFLRQQASQKKKTIIISDILQSGKDEDELYGEIAAII
                     RNKNIDRLIGIGPAISRQADKFSLEKSFFQNTADFLQRFSFAAFNNEVILLKGARVFE
                     FEQISLALQQKTHETVLEINLNALEHNLNYYRSVIRPGTKIMAMVKAFSYGIGSFEVA
                     NALQFHRIDYLAVAYADEGVELRKAGINVPIMVMNPDEESFDAIIQHSLEPEIYSHRI
                     FDKLENAIKKNILPKNKPVKIHIKLDTGMHRLGFLEEDLDRLIDMLKANRMIYVQSVF
                     SHLAASEDKKETDFTRCQIENFRRMASKITDQAGHPVMMHILNSAGITCYPEAQFDMV
                     RLGISLYGIATLEEEQPRLQPVGRLKSAISQIKTVAAGESIGYNRAFRAKKQIRMAIV
                     PIGYADGLSLALSNGKGHLMVGGKRVPVIGRVCMDMCMLDITGIEAEEGDEVIIFGPE
                     RPLQELAKEMGTIPYEVLAGLSRRVKRVYYHE"
     gene            2221..2955
                     /locus_tag="PKZ74_01585"
     CDS             2221..2955
                     /locus_tag="PKZ74_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91468.1"
                     /translation="MEIKRLSIIVPAYNEARTIHLILDKVLAVRLINDIEKEIIIVND
                     CSKDETEKVVNQYIEEHKEANVRLFNQPVNMGKGAALHRGIQEASGEYLIIQDADLEY
                     DPEEYNDLLKPVVNGFADVVYGSRFMGSKPHRILFFWHTIGNKFLTFISNMFTNLNLT
                     DMETCYKLFKSDYIKKIYFKENRFGFEPEVTAKISRFPKIRIYEVGISYYGRTYEEGK
                     KIGARDGFRAIWCILKYNLFSKRYLK"
     gene            2989..4458
                     /locus_tag="PKZ74_01590"
     CDS             2989..4458
                     /locus_tag="PKZ74_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91469.1"
                     /translation="MARQFNNYLTGIRDRMTGQQSILFLINSALFIAGILLLIGPAIQ
                     NGFPILHADSGTYLLEGFGTKIPVSRPLSYCLFVRFTSRIFSIWSVVVTQAIIAYWTI
                     WLAVNTLAGKKKIAWLPFITVALLSVTTGISFYVSQIMTDIFMPVALLGLFILVVRER
                     MPWYTWLFLGLIVWLSLIVHMSNLPVLTGLAFAIIILKIILEKSIKPVKQLSVYIIAG
                     ILVISWLTNPLISTIYGEGFRTSNSGNIVFFSRLLQAGAAQKYIKDRCEKEPEYYLCE
                     YKDQIDTYDRLDKFLWPDTSFLYDHPCREKTWDYCWRVRNQEFGEINSAILDHPPSRN
                     IYLKAVWDDFQLALHAFGLTGYVSFREGSHIDYPLKRYYKNDYKSFKNSRQYAGTMVF
                     RDMNRIIHVVVWISLALAAFLIIRNRRILDRRSPYLILMSILVLTWLGNALLTAFVAV
                     VSERFLGRFIWLLPFFVIMLVYREYSALYADSADKAREE"
     gene            complement(4469..5437)
                     /locus_tag="PKZ74_01595"
     CDS             complement(4469..5437)
                     /locus_tag="PKZ74_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM91470.1"
                     /translation="MTTYTLVTGANGYLGSYICRELISRGMPVIALRFDHYASVILEH
                     PLIEYVYCDITQPVLPQLESAIKGKKINTIINAAALLGSSDYEKNRKVNAGGVMNLIE
                     FAKSQGIKRFIQISSVVVIKTFKGPYGETKLIGQQYVEQSGLDYTVFIPAMILGPEGL
                     GINRILKNVFRFPLFVPLIGSGKQTQHPIFVRDFAKYIVLAIDNPSAIGKTYQIASDR
                     VISFRDLIRLVLKIKGKRRIFVSFPAPFARLLGKFFQKTQKVPLFTAEHVKGVLQDSN
                     LDTSLIRQDLGDHFTRLEDAMKYTLDEIGGNWDFYLKTRSEKVVKL"
     gene            complement(5494..6432)
                     /locus_tag="PKZ74_01600"
     CDS             complement(5494..6432)
                     /locus_tag="PKZ74_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM91471.1"
                     /translation="MAVKKFNWFTILRLAGVALFIVVLARTDLGELWSWIRTVDGTSL
                     FLAILFQLLVLLIKAWRWYNLNEAGFDARRLGRRSGEFFEGYAMGVITPGRMGELMKA
                     GHAGTRTGILGTGLRVIAERGMDLSIFIVVAGIAMSQGALPGVSNLWGWLVLAAGILG
                     MSLSLIILISPGMVRFAEYTMKMVRMLPKDKTLEFVSRTPWNSAGFVAWSLMSNLSFF
                     VCCYFLAAGVGIGLDIIHVSGGVAVAGVLNSIPVTVMGLGTREVTFLYVFSAFPRAQV
                     MAFSGLVFLVAQIGGGIISLVMGQILLFKYKANQVQ"
     gene            complement(6436..7749)
                     /locus_tag="PKZ74_01605"
     CDS             complement(6436..7749)
                     /locus_tag="PKZ74_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPM91472.1"
                     /translation="MKKPENIQDVIRHDLCNRCGSCVGLSGGKIVFENRTGSYLPVIK
                     EKPDKATEELIWQACPGKGFDFPEQRNAIYGEQEAHPYIGHYREIYIGFAADPETRLS
                     AASGGIISAILIWLLETGRIKGAVVLAMSEQEPWLTKPVIATTKEQILEAAQSKYIIS
                     SVNEILPEMERFDGPLAYVGLPGQVQSIRLLRQISHPSVRNIRYIFGPFYGNTLHFSS
                     VKSFLRSFKVKDYTLIRKLYFRHGEWPGKMRVELSDGRAFELPKFHANYLIPFHILKN
                     SLLCTDLTNEYTDISGGDAWAPVYEERGKGFSMVISRSEEGGKILREMADAGRLELSP
                     ITEKEAVDMHSHGYDLKKRGTFIRMKYRSILGKPNPDYGYIIKGFSVSRYLMEFIINV
                     LFLMAGTRFARWTVERIPPETIGKIFEKARKFWKKSTHNIKREKL"
     gene            complement(7736..8455)
                     /locus_tag="PKZ74_01610"
     CDS             complement(7736..8455)
                     /locus_tag="PKZ74_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM91473.1"
                     /translation="MIRKNTWTDKEVEAHWDNVADIYVAENNKVKDTHDQRFRETVHH
                     LRLEPGMKVLNITSRDAEADDYIRRANRDVEVINAEISSGLIDVAAKIRPAVKQVKIG
                     NYSILPFQDAEFDRVISLETLEHVAEPLRFLSELHRVSNPDARLVLSCPPATSELPYR
                     IYTALFGGHGEGPHRFLPSKEVKEMFAETKWKPVLHKGTILIPVGPSWLRNFGENIIG
                     RFQGTFIAELGIRQFFVCEKA"
     gene            complement(8452..9318)
                     /locus_tag="PKZ74_01615"
     CDS             complement(8452..9318)
                     /locus_tag="PKZ74_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91474.1"
                     /translation="MLNNPDITASIIIPVYNEDKGIGILLEKMTGMKLHETFEIIVVN
                     DGSTDNSLEVIRQYPVKVYTHTVNKGYGAALKTGIRKAGGAKVIILDSDGQHDPAQIR
                     EIAGMLDEHEMVIGTRNKDSFQVKTRQVGKKVIRWVGEYLVEQKLPDYNSGYRGFQRE
                     LIHSMLHMMPNGFSFSTTSTLAFLKEGYSIGTIPIKVEERVGRSSNVKFFKDGAKTMM
                     LILRIIMLFNPLKIFFPASFIFTTMGVLYGIYGYIIAERFSNGAIVMVILGMFLFFIG
                     LVADQISIMNRR"
     gene            complement(9325..10728)
                     /locus_tag="PKZ74_01620"
     CDS             complement(9325..10728)
                     /locus_tag="PKZ74_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91475.1"
                     /translation="MNKLFRTLGIHKIRLDYLALLIILSGLVYTNLYYKFWKQPGRII
                     AHDVILYYQYLPAAVIYKDLSLKFTSENPDFFKDKIWKIHTESGNGVSKMTMGLAVLY
                     SPFFLAAHAFAGKLGYPADGYSDPYRFMLILSSLVYAAFGFIFLIKLLNKYFARSTVA
                     LTILAIGLGTNLYFYTTIEPTMSHSYSFFLFAAFLFLVDAWIQKQSWVNTVFLGIVGG
                     LIILVRPTNGIVFILLLLWKIDSWRGLVDRIRLFLSLYLKTGAIAFLVILLFLPQVIY
                     WKILTGDFFFYSYRDEKFFFNDPAFLHGMFSYRKGWLVYTPMMIFSILGMIVMYFKHK
                     RLFWPVLTFTFLNLYIIFSWWCWWYGGGFGQRALIESYAVLAVPFAAFTDFILNRKLV
                     LKIIFLTLVTGFIALNIFQTRQYYKGVIHWDAMSKYTYWDSFFRTKVAPNFYNQVEKP
                     DYKAAMKGDRYEIMKQN"
     gene            complement(10798..13071)
                     /locus_tag="PKZ74_01625"
     CDS             complement(10798..13071)
                     /locus_tag="PKZ74_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPM91476.1"
                     /translation="MQSGRNKTSGKKGSNTYLRRFWIIYVLSMVLIVLFFAGIANNLF
                     GPLPSFEELENPKTSLATEIYSSDKKLIGTYYIENRSNAEYIELPQHLVQALLATEDI
                     RFEKHAGVDLKALFRVAFGVITGNSKGGGSTLTQQLAKNLFPRERDLSKPQLVMRKFK
                     EWITAIRLERNYSKQEILAMYLNTVDFGSQSWGIKIASKTFFSKPVDSLNLQESALLI
                     GVINAPTWYSPVRNPERSFERRNLVLRQMERYKFITKEVCDSVSNIPLDMTAFGLRGH
                     STGLATYFREILRMELKDWCSKHYKSDGTPYDLYKDGLKIYTTIDSRLQRYAEEAMTE
                     HLKNDLQPSFFAHWRRHVNAPFVFEPDVAAVEIKNLLEQSMKRSDRYRQLKEEGVPLD
                     SIRLIFNTPVKMRVFSWKGPVDTVMTPLDSIRYSKYFLQAGLMSMDPHSGYVLAYVGG
                     IDYRYFQYDHVKLSKRQVGSTFKPFLYSLAMQEGEFTPCSKIANVQYTVDLPDGTVWA
                     PKNTNKKRRGEEVTLKWALANSNNWISAYLIKRYSPQSVITMARKMGVTADLDPVPSL
                     ALGSSDISLYEMTGAFNTFANKGVHIKPIMIVRIEDKYGNVIESFVPEKQEAMSEETA
                     YLMLELMKGVVQSGTGIRLRTKYNFTNPIAGKTGTTQNQSDGWFIGITPELTTGVWVG
                     AEDRSVHFRTLGLGAGANTALPIWALYMKKAYADHSLRISQGDFERPSQPLSVSLDCD
                     KYEKEQDNRLEDEIIEF"
     gene            complement(13087..13599)
                     /locus_tag="PKZ74_01630"
     CDS             complement(13087..13599)
                     /locus_tag="PKZ74_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPM91477.1"
                     /translation="MTAGLLTGIKETARAFSRMMMPAAVFSLLVVTQGCGRPPLFDQN
                     KSFENHVWNLDDIIRFDVNVTDTVHIHKYFLNVRHSTDYRYANLYVFINSTFPDGRQA
                     RDTVECILAEPSGKWLGKGISTVRDNQILLRRGLRFPKTGNYIFEIEQAMREEELHGI
                     MDIGIRIERE"
     gene            complement(13602..14717)
                     /gene="ricT"
                     /locus_tag="PKZ74_01635"
     CDS             complement(13602..14717)
                     /gene="ricT"
                     /locus_tag="PKZ74_01635"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPM91478.1"
                     /translation="MEEDKKHSCGDAFLSRGCVCFPKMYDNNKHTFKPGCSKLDSSDW
                     LKDIDLPPGQKPFDCVEIRFKNSRKEFFRINTDYDIRQGDIVAVESSPGHDIGIVTLA
                     GEACRIQMKKKNVDPTLDTIKKVYRKARATDIEKWISCVEIEDDTMFKSRQAATDLDL
                     DMKISDVEYQGDGTKAIFYYTADERIDFRQLIKTLAEMFRVRIEMRQIGVRQEASRLG
                     GIGSCGRELCCSTWLSRFRSVTTQAARVQQLALNPQKLAGQCSKLKCCLNYEYDVYVD
                     TAREFPDTRIFLKTKKGICIHQKTEVFRRIMWYSYEGAPETLIPLPLDVVKEIIGMNN
                     KGKIPDKIEDFIQNKETKTLPQDGSDQFELHRFDKKK"
     gene            complement(14752..15930)
                     /locus_tag="PKZ74_01640"
     CDS             complement(14752..15930)
                     /locus_tag="PKZ74_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPM91479.1"
                     /translation="MLFRDITGQEEIKRRLIQTVKDNRVSHAQLFHGPEGSRKLAMAI
                     AYARFINCPNRSFGTKDGTMGDACGVCPSCIKFSKLIHPDLHFIFPVATTKEIEKDPI
                     SKDFLKTWREILLESNFRLSLNDWYKAANFEKKQGIINADDCSEILKTLSYKSYESEF
                     KVMIIWMADRLYHSAAPKILKILEEPPDKSLFILITENPEKIINTILSRTQAIKFPRL
                     ADDDLVWELTQKYGYPPGEAARVIPVADGNLTRAVKILKNDEDESFYTKTFVEWMRLC
                     YKHEMKHTMDFVADIAKIGREKQKNFLLYAERVIRGAFLSNFDHPALGRLNDEEKEFL
                     IKFGKFINHSNIVKFSEELEKAQVHIDRNANPSILFMDLSMTVTILLLTGQKAAASSQ
                     "
     gene            complement(15932..16708)
                     /locus_tag="PKZ74_01645"
     CDS             complement(15932..16708)
                     /locus_tag="PKZ74_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPM91480.1"
                     /translation="MDFTLKIWRQENAKAKGRFVTYQVTDISPDCSFLEMLDILNESL
                     VDKFEMPIGFEHDCREGICGTCALYINGRPHGPDKGITVCQMHMRRFKSGTTIVIEPW
                     RSRAFPVLRDLMIDRSAFDKIIQAGGYISIATGNAPDAHALPVEKLNSDLAMDAAACI
                     GCGACVAACKNASAMLFVSAKVSQLALLPQGRPEARKRVQAMVAKMDELGFGNCTNTY
                     ACEAECPKLIKITNIARMNREFFAAKVCGAKEVATTGGAG"
     gene            complement(16710..18650)
                     /locus_tag="PKZ74_01650"
     CDS             complement(16710..18650)
                     /locus_tag="PKZ74_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPM91481.1"
                     /translation="MTQLNSKIPSGPLADKWRTYKATQNLVNPANKRKIDIIVVGSGL
                     AGAAAAASLGELGFKVKCFTYHDSPRRAHSIAAQGGINAAKNYRNDGDSVYRLFMDTI
                     KGGDYRSREANVYRLAEVSNGIIDQCVAQGVPFAREYGGLLDNRSFGGALVSRTFYAR
                     GQTGQQLLLGAYGALSKEIEKGSVTMYTRRDMLDLVVIDGRARGIIVRNNVTGEIERH
                     SGHAVILATGGYGNVFFLSTMAMHSNGSAAWSAYKRGAFFGNPCFTQIHPTCIPVHGD
                     YQSKLTLMSESLRNDGRIWVPKKKEDAEKIRKGEMKPGDIPEEDRDYYLERRYPAYGN
                     LSPRDISSRAAKERCDAGYGVGSTGLAVYLDFADAIQRLGKGVIEARYGNLFQMYEKI
                     VDQNPYETPMMIYPAVHYTMGGLWVDYELQTTIPGLFVGGEANFSDHGANRLGASALM
                     QGLSDGYFVLPYTVQNYLADQFKVPRFSTDLPEFLAAEKEVRDNINRLLGIKGKKSVD
                     TFHRELGRIMWENVGMGRNKEGLEKALKLIPELREEFWKDVRIPGTNDELNPELQKAT
                     RLADYLELGELMALDALNREESCGGHFREEYQTPDGEALRNDKDFAYVAAWEYKGTEN
                     KAELHKEPLNYEFVELKQRNYK"
     gene            complement(18677..19357)
                     /locus_tag="PKZ74_01655"
     CDS             complement(18677..19357)
                     /locus_tag="PKZ74_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPM91482.1"
                     /translation="MSKFLRYSSLTKKFIMAFAGAFLMVFLVVHLGINLFIIPITPNH
                     KEIFREAVHFMTSNPLVKIMEIFLFGGFILHMILGVILQMQNWRSRPVRYKVEGWSHT
                     SFFSKFMIHTGVIIGIFLVIHLMNFYLVKAGITSAPEGPVRVTDDHDFYSMAVNLFSN
                     NTYSVIYIILIILLGFHLNHAFQSAFQSLGLDHSKYTPFIKAVGDLYSIVIPLGFCII
                     PLYFLVYH"
     gene            19530..20831
                     /locus_tag="PKZ74_01660"
     CDS             19530..20831
                     /locus_tag="PKZ74_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPM91483.1"
                     /translation="MKTITIDKDLFTINRRNFTERLLPDSLAIFHSNDIFPRNGDQTF
                     KFRQQSDLFYLSGIAQENTILLLYPDCPNPDLREVLFITEPTESLATWEGHKYSRKEA
                     QGISGIEKVRWTGQFDTVLTEVMSFAENVYLNAIEYAKFSTSVPYSDLRFAREIREKY
                     PNHTYRRSAPLVYSLRTIKSDEEIRLIREAINLTGKAFERVLKFVRPGVTEYQVEAEI
                     THEFLYEGASGSAYHPIIASGANACTLHYIENSSVCKEGELLLMDFGAEYGHYAADLT
                     RTIPVNGKFTERQKACYDAVLRVLRQASRLLVPGNTIDKVNKEANRLMEGEMTGLGLF
                     SREQAAQQDPEKPLYTKYFMHGVSHFLGLDVHDVGSKFEPFRPGMVFTFEPGLYIREE
                     NLGIRLENNYLVTEGEPLDLTAGIPVEAEEIEGMMSATPTG"
BASE COUNT         5093 a         5360 c         5301 g         5435 t
ORIGIN      
        1 accggcagca acggaaagac catcgttaaa gagtggctct accagttgct gagcaagagc
       61 cggaaaacgg tacggagccc gaaaagctac aattcccaga tcggggtgcc cctgtcggta
      121 tggcaaatgg atacacctta cgagatggct atctttgaag ccgggatttc ggaacccgat
      181 gagatgatcc ggctggagac gatcattcag ccgacgatcg gcgtttttac caatatcggc
      241 gaagcccacg gggagaactt catgagcctg cagcagaagg taggggagaa gctttttctt
      301 tttacccatg tcgaaacgct gatttattgt cttgaccatc ccgaggtgcg ggaggtcatt
      361 atccggtcgg gaatcagcaa gaatatcaaa accttcacct ggagccataa gcaggaggcc
      421 gacctccgta tccgggaggt gaggccggag aatgcgcata aaacgaatat ccgcgccgtt
      481 ctcaggggcc gggagatcag catcaccatc ccgttctccg acaacgcctc ggtcgaaaat
      541 gccattcatt gctggtcggt gatgctgttg ctcggttacg aggcggaatc gctcacaggc
      601 ctttttgaag cgctccagcc gattgccatg cgcctcgagt tgaaagcggg catcaatcat
      661 tgcacggtta tcaacgacag ctacaactcc gatatcaact cgctcagcat tgcgatcgat
      721 tttctccggc agcaggccag ccagaagaaa aagacgatca taatctccga tattctccag
      781 agcgggaagg atgaagatga actgtacggc gaaattgccg ccatcatccg caataaaaac
      841 atcgaccggc tgatcggcat aggaccggca atctcacggc aggccgacaa attcagcctc
      901 gagaagtcat ttttccagaa tacggccgat ttcctccagc ggttctcctt tgccgctttc
      961 aacaacgagg tgatcctgct caaaggggcc cgcgtcttcg aattcgagca gatcagcctg
     1021 gccctgcagc agaagaccca cgagacggtg ctggagatca atctcaacgc cctggagcac
     1081 aacctgaact attaccgttc ggtgatccgg ccggggacga agatcatggc gatggtaaaa
     1141 gcattttcct acgggatcgg cagttttgag gtggccaatg cgctgcagtt ccaccggatc
     1201 gactacctgg ccgtggccta tgccgacgag ggggtggaac tccggaaagc cgggatcaac
     1261 gtgccaatca tggtgatgaa cccggatgag gagagcttcg acgccatcat acagcacagc
     1321 ctggagcccg aaatatacag ccaccggatt tttgacaagc tggagaatgc cataaaaaag
     1381 aacatcctgc caaagaacaa gccggttaag atccatatta aactcgatac aggcatgcac
     1441 cggctgggat tcctggaaga ggacctcgac cggctgatcg acatgctgaa agccaaccgg
     1501 atgatctatg tccagtcggt attctcgcat ctcgcggcca gcgaagacaa gaaggaaacc
     1561 gactttaccc gctgccagat tgagaatttc cggaggatgg cttcgaagat aaccgaccag
     1621 gcggggcacc cggtgatgat gcacatcctg aattccgcgg ggatcacctg ttaccccgaa
     1681 gcgcagttcg acatggtacg gctggggatc agcctgtacg ggattgccac gctggaggag
     1741 gagcaacccc ggctgcaacc cgtgggcagg ctgaaatcgg ctatttcgca gattaaaacg
     1801 gtggcggccg gggaaagcat cggctacaac cgtgctttcc gggcaaaaaa gcagatccgg
     1861 atggcaatcg tcccgatcgg ttatgccgac gggctaagcc tggcgctgag caacggaaag
     1921 gggcacctga tggttggcgg caaacgggtg ccggttatcg gccgggtgtg catggacatg
     1981 tgcatgctgg atatcaccgg gatcgaagcg gaagagggcg atgaggtgat cattttcggc
     2041 cctgaacggc cgctgcagga gctggcaaag gagatgggca ccatcccgta tgaggtgctg
     2101 gccggtctgt cgagacgggt gaaacgggtt tattatcatg aatagcgcat tttatttccc
     2161 atcaaacgga atttttattt cttttgatga agattcggtt ttatgctcat aagatcatcc
     2221 atggaaatca aaagattatc gattatcgtc cctgcttaca atgaagcccg gaccattcac
     2281 ctgatccttg acaaggttct cgccgtcagg cttatcaatg atattgaaaa ggagatcatc
     2341 attgtgaatg attgttcgaa agatgaaacg gagaaggttg taaaccaata tattgaagag
     2401 cataaagagg ccaatgtccg gttattcaac cagcccgtca atatgggaaa aggggcagcg
     2461 cttcaccggg gcattcagga agcgtctggt gagtacctga tcatccagga tgccgacctg
     2521 gaatatgatc cggaagagta caacgacctg ctcaagccgg tcgtcaatgg atttgccgat
     2581 gtggtttacg gctccaggtt catggggagc aagccccacc gcatcctgtt tttctggcat
     2641 accatcggta ataaattcct caccttcatc tccaacatgt tcactaatct taatcttaca
     2701 gatatggaaa cttgttataa gcttttcaaa tctgattata tcaagaagat ctattttaag
     2761 gaaaaccggt tcggatttga acctgaagtt acagccaaga tttcacgctt tccaaagatc
     2821 aggatttatg aggtcggcat ctcttattat ggcaggacct atgaagaggg aaagaaaata
     2881 ggagcccgtg acggtttccg ggctatttgg tgcatcctta aatataattt gttcagtaag
     2941 agatacctga agtaaagaag ttccggaaat tttcaacaga agttgctcat ggccagacaa
     3001 ttcaataact atttgaccgg aatccgggac cgtatgaccg gtcagcaatc catcctgttc
     3061 ctgatcaatt cagccctctt tatagccggt attttgctcc tgatcggacc ggccattcag
     3121 aacggattcc cgatacttca tgccgactcg ggtacctacc tcctggaagg atttgggacc
     3181 aagatccccg tcagccgccc gctttcatac tgcctgttcg tcaggtttac ctccaggatc
     3241 ttttcaatct ggtctgtcgt cgtcacgcag gccatcattg catactggac catctggctg
     3301 gctgtaaata cgctggcagg caagaaaaag atcgcctggc tccctttcat taccgttgcc
     3361 cttttgagtg tcacgactgg aatttccttc tatgtcagcc agatcatgac cgatattttc
     3421 atgccggtag ccctgctggg tttgtttatc ctggtcgtca gggaaaggat gccctggtac
     3481 acatggctgt tccttgggct aatcgtctgg ttatcattga ttgtacatat gtctaatctt
     3541 ccggtattga cagggctggc gtttgcaatc atcattttaa aaataatact ggagaaatcg
     3601 atcaagccgg taaagcaatt gtcagtgtat attattgcag gcattctggt tatttcatgg
     3661 cttacgaatc cgctgatcag tacgatttat ggcgaagggt tcaggacgtc gaacagcggg
     3721 aatatcgttt ttttctcccg gttgttgcag gcaggggcag cgcagaaata catcaaagac
     3781 cgttgtgaaa aggaaccgga gtattacctg tgcgaataca aggatcagat tgacacatat
     3841 gaccggctgg ataagttttt atggcccgat accagttttc tttacgacca cccctgtcgc
     3901 gagaaaacct gggactattg ctggagggtg cgtaaccagg aatttggcga aataaattca
     3961 gcaattctgg atcatccgcc aagccggaat atttatctca aggctgtatg ggatgatttt
     4021 caactggctt tgcatgcttt cgggcttacg ggatatgttt cattccggga gggatcgcat
     4081 atcgattatc ctttaaagcg ttattacaag aatgattaca aatcctttaa gaattcacgg
     4141 cagtatgccg ggacaatggt attccgcgat atgaacagga tcatacatgt tgttgtatgg
     4201 atttcgctgg ctctggcagc ttttctgata atccggaacc ggagaatcct ggatcgtcgt
     4261 tccccttacc tgatacttat gagcatcctg gtcctcacct ggcttggaaa tgccctgctc
     4321 accgcttttg tcgctgttgt ttcggaaagg ttcctgggcc gctttatatg gctcttgcca
     4381 ttctttgtca tcatgctggt ttaccgagaa tacagcgcat tatatgcaga ctctgccgat
     4441 aaggcacggg aagaataagc tgaccagatt atagcttcac tactttttcg ctcctggttt
     4501 tcaggtagaa atcccaattc ccgcctattt catccagggt gtatttcatt gcatcttcca
     4561 ggcgggtgaa atgatcccca agatcctggc ggataaggga ggtgtcgagg ttggagtcct
     4621 ggaggacccc tttgacatgt tctgcggtga acaggggaac cttttgcgtt ttctggaaga
     4681 atttacccaa caacctggca aaaggagcag ggaagctgac aaagatcctt cttttcccct
     4741 tgattttcag tacaagccgg atcagatcgc ggaaagagat gaccctgtcg ctggcaatct
     4801 ggtatgtttt tccgatggca gaggggttat cgatggcgag tacgatatat tttgcaaaat
     4861 ccctgacgaa gatcgggtgc tgcgtttgtt taccgcttcc gatcaacggg acgaataaag
     4921 gaaagcggaa tacgttctta aggatccggt tgatgcccag cccttccggc ccgaggatca
     4981 tagcgggaat gaataccgtg taatcaagtc ctgattgctc aacgtattgc tgaccgatca
     5041 gcttggtttc gccataaggc cctttgaatg tttttatcac gaccacgctg ctgatctgga
     5101 taaacctttt gatcccctgc gatttcgcga attcgatgag attcatcaca cctccggcat
     5161 tcaccttccg gttcttttca taatctgaac tccccagcaa tgcggccgca ttgatgattg
     5221 tatttatttt ttttcctttg atggcagatt caagctgggg gagtaccggt tgggtaatgt
     5281 cgcaataaac atattcgatc agcggatgct ccaggatgac cgaagcataa tgatcgaacc
     5341 gcagcgcgat gacgggcatt cccctgctga tcagttccct gcatatgtag cttcccaggt
     5401 atccgttggc tcccgtaacg agggtgtagg tagtcataag tcgttagtcg ttaatttatg
     5461 tccggcttta aaagattatt cgttattcgt tattcactgg acttgatttg ccttatattt
     5521 gaacaggagg atttgtccca tcaccagtga gatgataccg ccgccaatct gggctaccag
     5581 gaagaccagg ccggagaagg ccatgacctg ggcccgcgga aaagctgaaa acacatacag
     5641 aaaggttacc tccctggttc ccagccccat aacggtgacc ggtatggaat tcaacacgcc
     5701 tgccacggca actccccctg aaacatggat aatgtcgagg ccgatcccta ctccggcagc
     5761 caggaaataa cagcatacaa agaaactcag gttgctcatc agcgaccagg ctacaaatcc
     5821 tgcagaattc cagggcgttc tgctgacaaa ctccagcgtt ttatcttttg gcagcatccg
     5881 gaccattttc atggtatatt cagcaaaacg taccattccc ggggaaatga gaatgatcag
     5941 cgacaggctc atgccgagga ttccggccgc gagcaccagc cagccccaca aattactgac
     6001 tccgggcagg gcgccctgcg acatcgcaat tccggcaacg acgatgaata tgctgagatc
     6061 catccccctt tcggcaataa cgcgtaaacc ggtgccaagt atcccggtcc gtgttccggc
     6121 atgtccggct ttcatcagct ctcccatcct accgggcgtg atcaccccca tggcatagcc
     6181 ttcaaaaaat tcccccgatc tccttcccag gcgacgggca tcgaatcccg cctcgttcag
     6241 gttgtaccat cgccaggctt tgatcagcag aaccagcaat tgaaaaagta tggcaagaaa
     6301 cagacttgtt ccatcaaccg tcctgatcca gctccataat tcacccaggt cggtcctggc
     6361 caggacgacg ataaaaaggg ccacacccgc caggcggaga atggtaaacc agttgaattt
     6421 tttaacagcc atcgctcaca acttctcccg tttaatatta tgcgtggatt tcttccagaa
     6481 cttccttgct ttttcaaaga tcttcccaat ggtctccgga ggaatccgct cgacggtcca
     6541 ccgggcaaaa cgtgttcctg ccatcagaaa caatacattg ataatgaact ccatcaggta
     6601 acggctgacc gaaaaacctt tgatgatgta gccgtaatcg ggattcggtt ttcccaggat
     6661 ggaacggtat ttcatccgga taaaggtacc tcttttcttc aggtcgtagc catgagagtg
     6721 catgtcgacc gcctcctttt ctgtgatcgg gctgagctcg agccggccgg catctgccat
     6781 ctccctgagg atctttcccc cttcttccga ccggctgatc accatcgaaa accctttgcc
     6841 ccgctcttca tagaccggcg cccaggcgtc tcctcccgat atatcggtat attcattggt
     6901 cagatcggtg cataacaggc tgttcttcag aatatgaaaa ggtatcaggt agttggcgtg
     6961 aaatttcggc aactcaaagg cccttccgtc gctgagttcc accctcatct tcccgggcca
     7021 ttcgccgtgc ctgaagtaga gcttgcggat caatgtataa tccttcactt taaaggatcg
     7081 aagaaaactt tttaccgagg agaagtggag tgtattaccg tagaacgggc cgaaaatgta
     7141 ccggatgttc ctgaccgacg gatggctgat ctgccggaga agacggatcg attgcacctg
     7201 tcccggcagg ccgacatagg ccagcggacc gtcgaatctt tccatttcgg ggagaatttc
     7261 gttgaccgaa ctgatgatat atttgctttg cgcagcttcg aggatttgtt ctttggttgt
     7321 ggcaatgacc ggtttggtca gccagggctc ctgctccgac atggccagca cgacggcgcc
     7381 tttgatcctg ccggtttcaa gcagccagat caggatggcg gagatgatcc cgccgctggc
     7441 cgcgctaagc cgggtttccg gatcggcagc aaaaccgatg tagatttccc ggtaatggcc
     7501 gatgtaggga tgcgcttcct gctctccgta aatcgcattc ctttgttccg ggaaatcgaa
     7561 ccctttcccc ggacaggcct gccagatcag ttcttccgtc gctttatccg gtttttcttt
     7621 aataaccggc aaataacttc ctgtccggtt ttcaaaaacg atcttcccgc ctgataatcc
     7681 cacgcaggag ccacaccggt tgcacaggtc gtggcgtatt acatcctgta tgttttcagg
     7741 ctttttcaca cacaaaaaac tgtcttattc ccaactcggc aatgaaagta ccctggaacc
     7801 tgccgatgat attctcaccg aaatttcgca gccacgacgg gccgaccgga atcaggatgg
     7861 tgcctttatg gagcaccggc ttccattttg tttccgcgaa catctctttt acttctttcg
     7921 atggcagaaa gcggtgcggg ccttcgccgt gcccgccgaa cagcgctgta tagatcctgt
     7981 atggcagttc gctcgtagcc ggcgggcagc ttaataccaa tcgtgcatcg gggttggaaa
     8041 cccggtgaag ctccgacagg aaacggagcg gctctgccac gtgttccagc gtttccaggc
     8101 tgataacgcg atcgaactca gcatcctgaa aaggtaagat ggagtagttg ccgattttaa
     8161 cctgcttgac tgccggtctg attttcgcag ccacatcgat gaggccgctc gagatctcgg
     8221 cgttaatcac ttccacatcg cggtttgccc gccggatgta atcatcggcc tccgcatcgc
     8281 ggctggtgat attcaaaact ttcatccccg gttccaggcg gagatgatgg accgtttcgc
     8341 ggaaacgctg atcgtgcgta tctttaactt tattattttc agcaacatag atatcagcca
     8401 cattgtccca atgcgcttca acttctttgt cggtccatgt attcttcctg atcatcggcg
     8461 attcatgatc gatatctggt cggccaccag gccgataaaa aacaggaaca tccccaggat
     8521 gaccatgaca atggccccgt tagagaaacg ctcggcgatg atatacccgt aaattccgta
     8581 caacacgccc attgttgtga atataaagct tgccgggaaa aatattttca gcggattgaa
     8641 caacatgatg atccggagga tgagcatcat ggtcttagcg ccatctttaa agaatttgac
     8701 attgctcgac cggccgacac gctcttccac cttaatgggg atcgttccga tggaataccc
     8761 ttccttcaga aatgcgagtg tagatgtggt tgaaaaggaa aatccgttcg gcatcatgtg
     8821 gagcatgctg tggatcagct ctctttgaaa tccccggtat cccgaattgt agtccggcag
     8881 tttttgttcg accaggtatt cgccgaccca gcggatcact tttttcccca cctgccgcgt
     8941 tttcacctgg aaggaatctt tgttccgcgt tccgatcacc atttcatgct catccagcat
     9001 gccggcaatt tcccggatct gggccggatc gtgctgcccg tcgctgtcaa ggatgatgac
     9061 cttggcgccg ccggctttcc ggatgccggt tttcaacgct gcgccataac ccttgttaac
     9121 ggtatgggtg taaactttga ccggatattg ccggatcacc tccaacgaat tgtcggtaga
     9181 gccatcattg acaacgatga tctcaaaggt ttcgtggagc ttcatgccgg tcatcttttc
     9241 cagaaggatg ccgatcccct tgtcctcgtt atagacaggg atgatgatgg aagccgttat
     9301 atcgggattg tttagcattg gagtttagtt ttgtttcata atttcatacc ggtcaccttt
     9361 catggccgcc ttataatcgg gcttttcaac ctgattataa aaattcgggg cgactttcgt
     9421 ccggaagaag gaatcccagt atgtgtactt actcattgca tcccagtgaa ttacaccttt
     9481 atagtactgc cgggtctgga atatgttcag tgcaataaaa ccggttacca gggttaaaaa
     9541 tatgattttc aataccagtt ttctgttcag gataaaatcc gtaaaagcgg caaaaggaac
     9601 ggccagcacg gcataagatt cgatcagggc ccgttggccg aatcccccgc cataccacca
     9661 gcaccaccag gagaaaataa tgtaaaggtt cagaaaagtg aaggtcaaaa caggccagaa
     9721 taatctctta tgtttgaaat acatgacgat catccccagt atcgaaaaaa tcatcatcgg
     9781 ggtgtaaacc agccatcctt tccggtacga aaacatgccg tgtaaaaaag cgggatcatt
     9841 gaagaaaaac ttttcatccc tgtagctgta aaagaaaaaa tcgcccgtaa ggattttcca
     9901 gtagatgacc tgaggaagaa ataataaaat gaccaggaaa gcgattgcac cggttttcag
     9961 gtaaaggctc agaaataatc ttattctgtc aaccagtcct ctccagctat cgattttcca
    10021 taataaaagg aggataaaaa caataccgtt tgtaggcctg accaggatga tcaatccccc
    10081 gacaatcccg aggaagaccg tgttaaccca tgactgcttc tgtatccacg catccaccag
    10141 gaaaaggaaa gcggcaaaaa ggaagaagct ataggaatga gacatggttg gctcaatcgt
    10201 cgtataaaaa tagaggttgg tacccaggcc gatcgccagg atggtaagag ctaccgtgct
    10261 tcgggcaaaa tatttattca gtagctttat cagaaaaata aatccgaatg cggcatatac
    10321 caggctggaa aggatcagca tgaaacggta aggatcagag tagccgtcgg caggatatcc
    10381 caattttccg gcaaatgcgt gagctgccag gaaaaacggg gaatacagaa cagccagtcc
    10441 catcgtcatt ttgcttaccc cattccccga ttcggtgtga attttccaga tcttgtcctt
    10501 aaaaaagtcc ggattttcag atgtgaactt cagactcagg tctttatata taacagcggc
    10561 aggcagatac tggtaataca ggatgacatc atgggcaata atcctgccgg gttgtttcca
    10621 gaatttgtaa tataaattgg tataaaccag cccggaaagg ataatgagaa gggccaggta
    10681 atcaagcctt attttatgta tcccgagagt tctaaacagt ttattcattt caaatgatcg
    10741 tgtatttggc caattcaggg tatttacact ccggaaaaat cagatccggt ggaagtatca
    10801 gaattcaatt atttcatctt caagccggtt gtcctgctcc ttttcatatt tatcgcaatc
    10861 aagggaaacg gataagggtt gcgaaggcct ctcgaaatca ccctggctga tccggagcga
    10921 atgatcggca taggcttttt tcatatacag cgcccagatc ggcagcgctg tattggcgcc
    10981 tgctcccagt ccaagcgtcc ggaagtggac gctgcggtct tccgccccga cccaaacccc
    11041 ggtggtgagt tccggcgtaa tgccgatgaa ccacccgtct gactggttct gtgtcgtacc
    11101 ggtttttccc gcaatcggat tggtgaaatt gtatttcgtc cgcaaccgga tacccgtacc
    11161 gctctgtaca acccctttca tcagctccag catcaggtag gcggtctctt cgctcatcgc
    11221 ttcctgtttc tcgggcacga acgactcgat cacattgccg tatttgtctt cgatcctgac
    11281 gatcatgatg ggtttgatgt gaacgccctt gttggcgaag gtgttgaagg cgccggtcat
    11341 ttcgtaaagt gagatatcag acgaacctaa agccagcgac ggaaccgggt cgagatcggc
    11401 ggtaacgccc atctttctgg ccattgttat gaccgattga ggggaatacc gtttaataag
    11461 ataggctgat atccagttgt ttgagttggc aagtgcccac ttaagagtca cctcttcgcc
    11521 tcttctcttt ttattggtgt ttttaggcgc ccaaaccgtg ccatccggca gatctaccgt
    11581 gtactgcacg ttggcgatct tgctgcacgg cgtaaactca ccctcctgca tcgccaggga
    11641 atacaggaag ggcttgaacg tcgacccgac ctgccttttg ctcagtttga catgatcgta
    11701 ctgaaaataa cggtagtcga ttccgccgac atacgccagg acgtaaccgg aatggggatc
    11761 catcgacatc aggccggctt gcaggaagta tttgctgtaa cggatcgagt ccaatggcgt
    11821 cataacggtg tcaaccggcc ctttccacga aaagacacgc attttgaccg gtgtgttgaa
    11881 gatcagccgg atggaatcga gcggtacccc ttcctctttg agctgacggt aacggtctga
    11941 ccgcttcatc gactgttcca gcaagttctt gatctccaca gccgccacgt caggctcaaa
    12001 tacgaatggt gcattgacat gccggcgcca gtgggcaaag aaagagggtt gcagatcgtt
    12061 tttcaagtgt tcggtcatcg cttcctcggc gtaacgctgc aaacgtgagt cgatcgtcgt
    12121 atagatcttc agtccgtctt tatacagatc atatggagtc ccgtctgatt tataatgctt
    12181 gctgcaccag tctttaagtt ccatgcgcag gatttccctg aagtaggtgg ccagccctgt
    12241 tgagtggccc cggagaccga atgccgtcat gtcaaggggg atgttggaga ccgaatcgca
    12301 aacttcttta gtgataaatt tatagcgctc catctgcctc agtaccagat ttctgcgttc
    12361 gaacgaccgt tccgggttcc gcaccgggct gtaccaggtc ggcgcattga tcacaccgat
    12421 cagcagggcc gattcctgga ggttcaaaga atcgaccggt ttgctgaaga aggtctttga
    12481 agcgattttt attccccacg actggcttcc gaagtcgacc gtgttgagat acatcgccag
    12541 gatttcctgt ttggaataat ttcgttccag ccggatagcg gtgatccatt ccttgaattt
    12601 acgcatgacc agttggggtt tcgacaggtc gcgttcccgc gggaataagt tcttggctaa
    12661 ttgctgagtc agtgtacttc cccctccttt gctgttcccg gtgatcaccc cgaaagccac
    12721 gcggaacaaa gctttcaggt cgacgccggc gtgcttctcg aaacggatat cctccgtggc
    12781 cagcagggcc tgaaccaggt gctgcggcag ttcaatgtat tcggcgttcg accggttttc
    12841 gatataatac gttccgatca gtttcttgtc agaggaatat atctcggtag ccaggctggt
    12901 tttaggattc tccaattctt cgaaagaggg cagggggccg aagagattgt tggcgatccc
    12961 cgcaaaaaaa agtacaatca gtaccattga cagtacatag atgatccaga accttctcag
    13021 gtaagtgtta ctgccttttt tgcccgatgt cttgttcctt ccggattgca tgtcattctg
    13081 gttaagttat tctctttcaa tacggatgcc gatgtccatg atcccatgta gctcctcttc
    13141 cctcatcgcc tgttctatct cgaagatata gtttccggtt tttggaaaac gcagcccacg
    13201 ccgaagcagg atctggttgt cgcggacggt gctgatccct ttcccgagcc atttgcccga
    13261 tggttcggcg aggatgcatt cgacagtgtc gcgggcttgc ctgccatccg gaaaagtact
    13321 gttgatgaac acatacaggt tggcgtaacg ataatcggtc gaatgtctca cattcagaaa
    13381 gtatttatgg atatgtaccg tatcggttac atttacatcg aaccggataa tatcatccaa
    13441 attccaaacg tgattttcaa aggatttatt ttgatcaaac aatggcggtc ggccacaacc
    13501 ctgcgtgacg accagcaatg agaataccgc cgccggcatc atcatcctgc tgaaagccct
    13561 tgccgtttct tttatgccgg tgagcaggcc tgcagtcatc ttcatttctt tttatcaaat
    13621 cggtgaagtt cgaactggtc ggagccatcc tgaggcagtg ttttggtctc cttattctga
    13681 ataaagtctt caattttatc cggaattttt cctttattat tcatgccgat gatctccttt
    13741 acgacatcca gcggcagcgg gatcagggtt tcaggggcgc cttcgtatga ataccacatg
    13801 atccttctga atacttccgt tttttggtgg atacaaatgc ctttttttgt tttcaggaag
    13861 attcgggtat cggggaattc ccttgccgta tcgacataaa catcgtattc ataattcagg
    13921 cagcatttca gcttgctgca ctggccggcc agtttctgcg gattcagggc aagttgctgt
    13981 acacgtgctg cctgtgtcgt caccgacctg aagcgtgaaa gccaggtgga acagcacaat
    14041 tcccggccgc atgagcctat gcctcccagg cggctggcct cctgcctgac gccgatctgg
    14101 cgcatctcga tccttaccct gaacatctcc gccagggtct tgatcagttg cctgaaatca
    14161 atccgctcat cggcagtgta ataaaaaata gctttcgtgc catcaccctg gtattccaca
    14221 tcgctgatct tcatatccag gtccaggtcg gtggccgcct gccttgattt gaacatcgta
    14281 tcgtcttcga tttccacaca ggatatccat ttttcgatat ctgttgccct ggctttacga
    14341 taaacttttt tgatggtatc gagcgtggga tcgacattct ttttcttcat ctggatgcgg
    14401 catgcctcgc cagcgagggt aactatgcca atgtcgtgcc ccggtgatga ctccaccgct
    14461 acaatatccc cctgccgtat gtcgtaatcg gtgttgatcc tgaaaaattc cttccggctg
    14521 ttcttgaatc ggatctccac acaatcgaac ggcttctggc cgggtggcag gtcgatgtcc
    14581 ttcagccagt cggaactgtc gagcttcgaa cagcccggct taaaggtatg cttgttgttg
    14641 tcgtacattt tcggaaagca aacgcatcct ctcgacaaaa acgcatcccc gcagctatgt
    14701 tttttatcct cttccatcag gatcaatttt cgacaaaaat acaaataatg atcattggct
    14761 gcttgctgcc gctttctggc cggtcagcag taaaatcgtc acggtcatcg acaggtccat
    14821 gaataatatc gacggattgg cattccggtc gatgtggacc tgggcttttt caagctcttc
    14881 gctgaatttc acaatattcg aatgattgat gaatttcccg aatttgatca ggaattcttt
    14941 ctcctcatca ttcagccggc ccagggccgg atggtcgaag ttcgaaagaa aagcccccct
    15001 gataaccctt tcggcataca ataaaaaatt cttttgtttt tcgcgtccga tttttgcgat
    15061 gtcggctaca aaatccatgg tgtgcttcat ttcatgttta tagcatagcc gcatccattc
    15121 aacaaaagtc ttagtgtaaa atgactcatc ttcgtcattt ttgagaattt taaccgcacg
    15181 cgtgagattt ccgtcggcta ccgggatgac ccgcgccgct tctccgggtg gataaccata
    15241 tttttgcgtc agttcccaca ccaggtcatc atcggccagc cgtgggaatt taatggcctg
    15301 ggtcctcgaa aggatcgtat tgatgatctt ttccggattt tccgtgatga ggatgaaaag
    15361 tgatttgtcg ggcggttcct ccagaatttt caggattttt ggggcggcgg aatggtacag
    15421 ccggtcggcc atccagatga tcatgacttt gaattccgac tcgtagctct tgtaactgag
    15481 tgttttcaaa atttcactgc aatcatcggc attgatgatc ccctgttttt tttcaaaatt
    15541 ggcggctttg taccaatcgt tcaggctgag ccggaagttt gattccagta gtatttccct
    15601 ccaggtcttc aggaagtctt tgctgatagg atctttttca atctcttttg tcgttgctac
    15661 cggaaatata aaatgcagat cggggtggat gagcttggag aatttaatac aggatgggca
    15721 tactccgcac gcatcgccca tcgtaccatc cttcgttccg aatgaccggt tggggcaatt
    15781 gataaaccgg gcataagcga tagccatggc cagtttacgc gacccttccg ggccgtggaa
    15841 caactgggca tggctgaccc tgttatcttt aacggtctga ataagccggc gcttgatctc
    15901 ttcctggccg gtaatatctc tgaaaagcat cctatcctgc ccctccggtc gtggccacct
    15961 ctttcgcgcc gcaaaccttt gctgcgaaaa attcgcggtt catccgggca atattggtta
    16021 tcttgatcag tttcgggcat tccgcctcac aggcatacgt gttggtgcag ttgccgaatc
    16081 ccagctcgtc catcttggcc accatggcct ggacgcgctt ccgggcttcc ggcctgccct
    16141 gtggcagcaa ggcaagctgc gacactttgg ccgacacaaa gagcatggcc gaagcattct
    16201 tgcacgcagc aacgcaagcg ccgcaaccga tgcaggctgc cgcgtccata gccaggtccg
    16261 aattcagctt ttcgaccgga agtgcatgtg catccggggc attgcccgtg gcgatggaaa
    16321 tatagccccc ggcctggatg atcttatcga atgccgaccg gtcgatcatc aggtcgcgca
    16381 gcaccgggaa ggctctcgac cgccagggct cgatcacaat agtcgtcccg ctcttgaacc
    16441 ggcgcatatg catctggcaa actgtaatgc ctttgtcggg gccgtgtggc cggccgttga
    16501 tatataatgc acaggtgccg caaatgcctt cccggcaatc atgctcgaac ccgatcggca
    16561 tctcgaactt gtcaacgagg ctttcgttca gaatatcaag catttcaagg aaagaacaat
    16621 cgggagaaat atcggttacc tggtatgtca caaagcggcc tttcgctttg gcgttttcct
    16681 gccgccatat ttttaacgtg aagtccatat tatttgtagt ttctttgttt cagttcaaca
    16741 aattcgtaat tgagcggttc cttgtgcaat tccgctttat tttccgtgcc tttgtattcc
    16801 caggccgcta cgtaggcaaa atccttgtcg ttgcgaagcg cttcgccgtc aggggtctgg
    16861 tattcctcac ggaaatggcc gccgcagctc tcctcgcggt tcagggcgtc gagcgccatc
    16921 agttcgccca gttccaggta atcggccagc cgggtggctt tctgcagttc gggattgagc
    16981 tcatcgttcg taccgggtat acggacatct ttccagaact cttctctcag ctccgggatc
    17041 agtttcaggg ctttttccaa tccttccttg tttcttccca tcccgacatt ctcccacatg
    17101 atccggccca gctcacgatg gaaagtatct accgatttct ttcctttgat gcccagcagg
    17161 cggtttatat tgtctctcac ctctttttcc gctgccagga attccggcag atcggttgag
    17221 aaccggggta ccttgaactg gtcggccagg taattctgga ccgtgtaggg cagtacgaaa
    17281 tagccgtcgc tcaggccctg catcagggcc gaagcgccca gcctattggc gccgtggtcg
    17341 gagaagttgg cttcgccgcc gacgaacagg ccgggaatcg tggtttgcag ttcataatcg
    17401 acccacaggc cacccatggt gtaatgcacc gccgggtaga tcatcatcgg ggtttcatag
    17461 gggttttggt cgacgatctt ttcgtacatc tggaacaggt tgccgtagcg ggcttcgatc
    17521 acccctttgc ccagccgctg gatggcatcg gcaaaatcga ggtaaacggc cagaccggtc
    17581 gagccgacgc cataaccggc atcgcaccgt tccttggcag cccgcgagga gatatccctg
    17641 gggctgaggt tgccgtaagc cggatatctt ctttcgaggt aataatcgcg gtcttcttcc
    17701 ggaatgtcgc ccggtttcat ttcgcccttg cggatctttt cggcgtcttc tttctttttc
    17761 ggcacccaga tgcggccgtc gttgcggagg ctttcgctca tcagggtcag tttcgactgg
    17821 taatcgccat ggaccgggat gcaggtcggg tggatctgtg tgaagcaggg attgccgaag
    17881 aaggcgcccc gtttgtacgc cgaccaggcc gcactgccgt tggagtgcat ggccattgta
    17941 gagaggaaga agacattgcc gtatccgccg gtggccagga tcacggcatg gcccgaatga
    18001 cgctcgatct cgccggtaac attgttgcgg acgatgatgc cgcgcgccct gccgtcgatc
    18061 acgaccaggt cgagcatatc acgacgcgtg tacatggtca ccgagccttt ctcgatctct
    18121 ttgctgaggg cgccgtatgc gccgagcagt agctgctgcc cggtctggcc gcgggcataa
    18181 aaggtgcggg aaacgagcgc gccgccgaag gagcggttgt cgagtaaccc gccatactcg
    18241 cgggcgaacg gaacgccctg ggctacgcac tggtcgatga taccgttact gacctcggca
    18301 agccggtaca cgttggcttc cctcgaacgg tagtcgccgc ctttgatcgt atccatgaac
    18361 aggcggtaaa cgctgtcgcc gtcgttacgg taattcttcg ccgcattgat ccctccctgg
    18421 gctgcaatgc tgtgtgcgcg gcgcggactg tcgtggtaag taaaacactt cactttaaaa
    18481 ccgagctcgc ccaggctggc ggcggcggcg gctcctgcca gccccgaacc caccacaatg
    18541 atgtcgatct tgcgcttgtt ggccggattg acgaggtttt gcgtcgcttt ataggttctc
    18601 catttatcgg ccagtgggcc tgaaggtatc tttgaattca gttgagtcat aatgcgggtc
    18661 aattaatctt tataaattaa tgatatacaa gaaaatacaa cgggattatg cagaagccga
    18721 gcgggatgac gatcgaatag agatcgccaa ccgctttgat gaacggcgta tattttgaat
    18781 ggtccaggcc cagcgactgg aaagccgact ggaaggcatg attcaggtgg aatcccagca
    18841 ggatgatcag aatgatgtag atgaccgagt aggtattgtt ggagaacagg ttgaccgcca
    18901 tgctgtagaa gtcatggtcg tcggtaaccc tgaccggccc ttccggagcg ctggtgatcc
    18961 ccgctttgac caggtaaaag ttcatcaggt gaatgaccag gaagatgccg atgatgaccc
    19021 ctgtatggat catgaatttg gagaagaatg aggtgtgcga ccatccttcg accttatacc
    19081 tgaccggcct cgacctccag ttttgcatct gtaaaatcac tcctaatatc atgtgaagga
    19141 taaatcctcc gaacaggaat atttccatga tcttgaccaa agggttggat gtcatgaaat
    19201 ggaccgcttc ccggaagatt tccttatgat tgggtgtaat gggaataata aacaggttta
    19261 tccccaggtg tacgaccagg aaaaccatca ggaatgcccc ggcaaaagcc atgatgaatt
    19321 ttttggtcaa tgaagaatac cgtagaaatt tactcatatc aaaataattt gaaattatct
    19381 taaaatattg ttaaacaagt gtatagcaaa agaatgttaa tcgattaaca aatttaatga
    19441 aattattaaa tatggtattt ttgagggatg aaatcggcta atttatattg aatccaattt
    19501 caaaaccaga gtaattaata taagttaata tgaagacgat caccatcgat aaagatttat
    19561 ttacgatcaa caggaggaat ttcacggaga ggcttcttcc cgacagcctg gccatatttc
    19621 attccaacga catttttccg agaaatggcg atcagacatt taaattccgg caacagtccg
    19681 atttgttcta tctctcaggc attgcacagg aaaatacgat tctgctgctt tatcccgatt
    19741 gtccgaatcc tgatctgaga gaggtcctgt ttattaccga accgaccgaa tcgctggcca
    19801 cctgggaagg gcacaagtat tccaggaaag aggcgcaggg catttccggt atcgaaaaag
    19861 tgagatggac cggccagttc gatacggttc tgacagaagt catgtcattt gccgaaaacg
    19921 tctatctgaa tgccattgag tatgcgaaat tttcaacatc tgtgccttac agcgatctgc
    19981 ggtttgcccg ggagatcagg gaaaagtatc cgaaccatac ttacaggcgt tcggctccgc
    20041 tggtttattc tttaaggacg atcaagtcgg atgaagagat ccggttgatc agggaggcga
    20101 taaaccttac gggaaaggct tttgaacggg tattgaagtt tgtgcggccc ggcgtgaccg
    20161 aataccaggt tgaggcggag atcacgcatg agttccttta cgaaggcgct tccggttcgg
    20221 cttatcatcc aataatagcc agcggtgcga atgcctgcac gctgcattac atcgaaaaca
    20281 gttcagtctg taaggaagga gaactgttgc tgatggattt cggcgccgag tatggccatt
    20341 acgcagccga cctgacccgt accatcccgg tcaacggcaa attcacggaa cggcaaaaag
    20401 cctgttacga tgccgtttta cgcgttttaa ggcaggcttc ccgtctcctt gtccccggca
    20461 acacgatcga taaagtgaac aaagaggcga acaggctgat ggaaggagag atgaccgggc
    20521 tggggctttt cagccgggaa caggccgctc agcaggatcc ggaaaagccg ctctatacga
    20581 agtatttcat gcatggcgtc agccattttc tcgggctcga cgtgcacgat gtcggctcga
    20641 agttcgagcc ttttcgtccc ggcatggtct ttactttcga accggggctc tacatccgtg
    20701 aagaaaacct gggcatccgc ctggagaaca attatctcgt aacggaaggc gagccgctgg
    20761 atctgacagc agggattccg gtcgaagcag aggagatcga agggatgatg agtgcaaccc
    20821 ctacggggta gattgaactt tgtctcggca ttgctaccag accggatgcc ctacgggcaa
    20881 tgtgcaaacc aggtgcatta tcagcgtttg gttcgataag gtgaaaccgg ataatagcct
    20941 tgcccgtagg gcatccacta tggtagcaaa cagcaccaac cattttcata ccctgtaggg
    21001 gtttaagatt aatcggataa ttattcctaa tttagcttcc tcaatctaaa cacaaatcgt
    21061 atgttctcat caaacgtcta tcttgcccgc cgaaacaggc tccgctcact tgtttcatca
    21121 gggattatcc tgttccccgg aaatccctga tgaaacaagt gagcggagcc tgtttcggcg
    21181 ggcaagata
//
LOCUS       DAOPNH010000014        21104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2355_length_21104_cov_6.729346, whole genome shotgun sequence.
ACCESSION   DAOPNH010000014 DAOPNH010000000
VERSION     DAOPNH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_21104_cov_6.729346"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..523
                     /locus_tag="PKZ74_01665"
     CDS             152..523
                     /locus_tag="PKZ74_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91484.1"
                     /translation="MMKKTTIFILLVFLALGLSAQKKALLEDKDQIIERAKLELDSAM
                     MAPEGYIYLQTVKNPIKGEYTFDISIWEKGTVTSLFAVGNNNGTLEAQNRLRAILMEF
                     KFNFKMPKGKSYKFRHTFVFN"
     gene            556..2196
                     /locus_tag="PKZ74_01670"
     CDS             556..2196
                     /locus_tag="PKZ74_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPM91485.1"
                     /translation="MKKQILSFFAVLAFLSIYAQEDMTMVWETKSPHKITFNGTGTEE
                     RGYSYVASDKEFSLFDNATGKIKWTKVYKEIAPKLSKIDELIPFWESNTVFLFDRKMG
                     KDQIACVDMETGSLLWNTDKYQNVGEENVVYIPEREGFAISLKDKLVFIKVATGEELW
                     STAKFGGVVGKYVFTPDGFLTTVNFQPSGLIALFTGFKNQIAKINMDNGDVVWSNTYI
                     GRAERKIISREFLYDIAVEGPRVILRLNGIQVYDYNTGANLWSAAFDFTPNGVRSRPP
                     NARQWGVYGAVADPVFDKENNVIYVLDMSNRNNQFVKKYDANTGKLIWTSPEIKGAKA
                     IPGMYVVDDKVILQIGGIVEFQSYEVIQTQYSTITIWRVFYDNVKPNGLQAFNTKDGS
                     LAWESEKFKKGITNAYVLENKILCSSGKELYKIDYKTGNEEYSVPVTDGGVGLAVKIL
                     PYKDKMIVVGEKGISAYNPVDGKFIYSGKYKASSLEDVFDNILIMKTEKADIASFNLD
                     TGVFKEFKAKTGASTTLTTDGKFVYIYEDKVFTKVKTL"
     gene            2290..3618
                     /locus_tag="PKZ74_01675"
     CDS             2290..3618
                     /locus_tag="PKZ74_01675"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM91486.1"
                     /translation="MIKLKDIRPIVQGDKKVIRGWVMYDWANSVFQLTITTAIFPLYY
                     DAITRNGGSYTVSFFGAEVINTVLYSWTIGAAFLMVALLSPLLSSIADYTGRRKAFMQ
                     VYTWVGALGCSMLFFFTGDNIELGIIAFFFGTWGYAGSIVFYNSFLPVIAKPEDHDKI
                     SARGYSMGYLGGVVLLLMQLVVIQKPSWFNIPAGSDLQFRLAFLTVGIWWIGFSQITF
                     SRLPKYVFKKRQGKENVILNGYKELRTVYYQVRKSKKLTLYLVSYFFFMMGLLSVMYM
                     AASFGKKEVNLGDEIMIPTLLLIQLIGMGGAWFFARLSGKIGNLKALIIALITWILIC
                     SGAYFITDAIGFITAAFFIGIVMGGTQALARSTYSKMLPETTDHTSFFSFFDVMEKMA
                     SAGGTFSFGLIEAITGNMRASVVAIAIFFIIGLFFIIVMPKRGTQNSAEI"
     gene            3682..7299
                     /locus_tag="PKZ74_01680"
     CDS             3682..7299
                     /locus_tag="PKZ74_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM91487.1"
                     /translation="MIFTPKLLRNTTILIFILLIFKITYSQSRQDNPVVWTGITSDTP
                     MESRIKLVSSDITTNRIEIITEGFLKEIIRDNEGGKEVVVSAENTTPLLIEGAPDLGK
                     LSIPLAIPDLDRMTVTVVEADFIDFQNILVIPSKGNLTRDINPDDVPYTYGREYRTDA
                     FFPGVAAELRTPHIIRDVRAQVVVAYPFRYNPVSKVLRVYTRLVLDIASAGEDGENPY
                     VLRREDGKTVRSFEEIYQRKLLNYKEYRTASRYTPLEEDGNMLIICYGSFMADMQPFV
                     DWKNMSGIPTEMVNVSSVGTTAAAIKSYVANYYNTNGLTYLLLVGDAAQVPVSSTTAG
                     PSDNDYGYIVGSDHYPDIFVGRFSAETNAHVQTQVQRTLNYEKQPDLSPGFFERAMGI
                     ASAEGPGDDGEYDWQHQRNIRTDYMGFTYTYGAELYDGSQGGQDAAGNPTATMVASEI
                     NTGTGIISYTGHGSNTSWSTTGFSNTNITSLTNTNKLPFILSVACVNGNFAGITCFAE
                     AWLRATYNNQPSGAVATIMSTINQSWNPPMQGQDEMVDILVENFPSNIKRTFGGICMN
                     GCMGMNDVYGSAGADMTDTWTIFGDPSLMVRTAAPVTIAVDHPADLPVGSDEITVLCD
                     QEGARVAVSRDFELLGSGYVSGGTALVEFGQINTEDTLIVTVTAFNCMPYIDTVFVTN
                     AEMVYVSSQAFHGNLTEVAPGQQDMEALGVKILMSGSVNPFNLLSLTFNMNGTTDLED
                     VESLAVYYTGNNGSFGTATLFGIGVVSAGSITVTGNQPLNGGFNYFWLAYDLAEDALM
                     GHFLDAECTGMALGDNPALTRVPAITDPDGQREINRMSLVSLTCSQPVTSPVPPGTVN
                     CEIIRLEITTDGSPLPLTVTGIKVATQGCTDLDDISAIKVYYTDLPAFSTTNLFGSVA
                     PASFVTVTGNRALTEGSDYFWIVFDISSAAGPGNELDAECTEVTFSGATGTQVPLVTD
                     PPGYRSIMLNYCTPTYTYGSGDGDYISLVSIGSLSKATGASSSPYYNYYQDETTHLVP
                     GSTYQLTLSPGTYGSGNNMAAWIDYNRNGTFESSEKLGELTNMSPTPATGTLTFTVPV
                     SAIHGILRLRVREVWNTTGLDPCAGYDYGETEDYDVHIMPPGCWLGYTNEWTNVSNWS
                     DGVVPGPGTEVIIPEILMGSYYPAVFGNSNLEIEQLNMQEGSNLSIPVGVTITVGGNR
                     K"
     gene            7359..8837
                     /locus_tag="PKZ74_01685"
     CDS             7359..8837
                     /locus_tag="PKZ74_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPM91488.1"
                     /translation="MVVKIIVLAIYALVIIIVGIYGLRKTRSFNDFFLGGGKVGAIMS
                     AFSYGATYFSAVVFIGFAGKIGWGFGYSGIWIGVLNGLIGVLAVWALLGWRVKKMSVE
                     MGVHTLSEYLEKRYGSPALKLIASIAIFIFLIPYSAAVFIGLSYLFASSFEGLQYWHA
                     VLFMGVFTAIYIVLGGYKSMAMMDTVFGIIMTTGVLVLFGYTLNAGGGLAAITEKLQS
                     IDPGLTATVGPPGWWPLLSLILLTSVAPFAMPQLMQKFYAIKDRKAIRTGMIASTIFA
                     LLIGGIAYFLGSTTRIFLAPETSPAAFTAEGKPVFDALMPELLARVIPESLSIIILLI
                     ILAASMSTLASLVLISSSSFCKDFYAGFINRNVTDKQLTNLMRGANLFFVLLSVILAL
                     IKPSTIVTILAMSWGAIGSAFLGPFIWGLFWKRTTRLAAFASMLLGLGTCIGLSIAGF
                     GAPEAGTIGMGVSLAVPPVVSWAGGRQLVLRAKPEGSAVGGR"
     gene            complement(8856..9851)
                     /locus_tag="PKZ74_01690"
     CDS             complement(8856..9851)
                     /locus_tag="PKZ74_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91489.1"
                     /translation="MTGTAASQTTLELNKEKERYAFGQIYTGFRYGFKDTYKPQAAFE
                     FNQGIIGYFHQIAPKVSGKIMFDVTRTTNITSIKDSSGQSMTVTYFEGSKYTAYLKMA
                     EIKWDINDYIALRVGQLLNTQYLTFQDPFWGYRYIDVTFQERYRLGQPADFGAQLDVK
                     VKDKFLNQFSVVNGEGPFRHQDLNGQFIFSNNIQYTPVKNLTLKLYADYGPAVDTGSV
                     YADKWVISGFAGYKTQKFRFGGEYDFVNNYGWMNGKDYSGFSVFGGWNFTGKFDILVR
                     WDHLKINAAGVEDILNYYIAGIQYEAARMFTTSVNFRYQSLEDLPSIYASFGLKF"
     gene            10050..12146
                     /locus_tag="PKZ74_01695"
     CDS             10050..12146
                     /locus_tag="PKZ74_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91490.1"
                     /translation="MKKHYIFTCFIAIFLTLPLLNLYAQKPGIYFEPSSLAETLAPDD
                     SVVVHSVLHNGSAQTVEFSFPGYVTRDLGGPDDFGYSWIDSEEPGGPDWSWTDISEVG
                     ILVEGLGDDEMAGPFEMGFNFSFYGQTKNHFWISPNGVISFNEQFIPYANEPIPTNDN
                     YNDFIAWFWDDLKIDSAITRVYYKNYKEKTVVQFTKMVHYPGTESFITGQVIMMVNGS
                     ILLKYRLVSEDFETTGATAGIQSWNSQQGLQVVYNAEYVHSELAVRFDLNRNFITSVN
                     PSTLTLPPGTQETIWITYSSTGFEVGSYEQDLKCITSHPELPHLILHNVMHVNVPVNS
                     GFKGYVTDAATGFAIHEVMVKAGEHVTYTNDNGYYELPLEEGVYNVKFQRNGYQTVIV
                     EDTTALEGYSILDVEMSGYYFIAGQVFADDNAIGSGFAYWYKLVEEEVVNIGAGMVGE
                     LGWYEFSSMQAGNYIIKAEPAPGSDYYELYLPTYYGDVLHWEEATIIELNLSTDDAHI
                     NLVAATVATEGPGTVSGKIESNTRTAEIPVFLRTLDHAIVYMAVTGTDGAYSFNNIPY
                     GTYEVFAEIPGKSITPKTIIIDELHTVIENIDMMIMDTEIIFLGIQESDIFIHEPFIY
                     PNPAKETLNVFTGLKKKTRVEITIFDVAGRIVTERTLEAGADETIRLDASFLPSGLYL
                     LKLASENDLRVIEFLK"
     gene            12216..13271
                     /locus_tag="PKZ74_01700"
     CDS             12216..13271
                     /locus_tag="PKZ74_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM91491.1"
                     /translation="MKNLNRSKSILAFIILIPIFFACNNKGNEPAKPAAAKPVEVRAV
                     IAEPKPVENKIFSTGSILANEEVEIRAEVPGRVTRINFQEGSAVKKGDLLVKINDNEL
                     LAELKKLELDEKLASDDVFRKEKLIELKAVSQEELDIARGQLGTIQAQIELVKARLEK
                     TEIFAPFAGRIGLRYISPGGYISSAMLIARLLQTDPVKIEFTVPEKYIGSVSQGMEVA
                     FTIAGSDSVFSGQVYAIEPRIDPSTRSFTVRARCQNPGGVLVPGAFAKVTAILERIPD
                     AIVLPSDALIPDIKGEKAYLCRNGKATAVYVTTGIRTETDVQITSGINPLDTVITSGL
                     LQIREQMSVTPRINGKN"
     gene            13287..16367
                     /locus_tag="PKZ74_01705"
     CDS             13287..16367
                     /locus_tag="PKZ74_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM91492.1"
                     /translation="MNISSISINRPVLATVISILLVLFGAIGYTFLGVREFPSVDPPV
                     VTVSTSYIGANADVIESQITEPLEESINGIAGIRSLTSTSSDGRSNITVEFELGVDME
                     AAANDVRDRVSRAIRSLPPDTDPPVVTKSDADASPILVLMLQSNERNLMELSDLANNV
                     FKERLQTVPGVSEIRIWGEKKYAIKLLMDPAKLASYQLTPVDVRNALTRENVELPSGR
                     VEGYRTELTIRTLGRLTTPEEFNDMIIRESDGTLVRFRDVGKAVLVPENERSIMRGNN
                     AVPLVGVAITPQPGANQIAIADECYKRLDQIKLDLPEDISVGLAIDTTKTIRNAITEV
                     FETLLIAFGLVVMVIFIFLRTWRTTLIPVIAIPISLVSGFFIMYIAGFSINILTLLGI
                     VLATGLVVDDAIVVLENIYHKVESGMNPVEAGHKGSKEIYFAIISTTITLAAVFLPVI
                     FLQGLTGRLFREFGMVVAGTILVSALVSLTLTPMMSSRMLRKTEKHSRVFNRTERWID
                     GLISGYNKRLTSFLRYRWLAILIMVLSIGMILGIGSLVPSELAPMEDKSRLSIVSTAP
                     EGTSFEVMDEYMLSLLEVVDTIPEKTNFLAITAPGFGSSASTNTGFFRVILTDPSERE
                     RSQQEIADELSQITRRYNFARSFVTQEQTIGGGRGGGLPVQFVILAPNFDKLRAVIPR
                     FMEEAQKSPLFQVTDLNLKFNKPEVSIDIDRNRARALGVNVRDVAENLQLYFSGQRYG
                     YFILNNKQYQVIGQADRLNRDEPLDLSSVYVRNNRNELIQLDNMVTLSENSNPPQLYR
                     YNRYVSATVSAQPAKGITLGQGIDEMRRIAGETLDDSFSTELAGVSKEYEDSSSSLLF
                     AFLLALVLIYLILAAQFESYIDPLIIMFTVPLALAGAVLSLWLFGQTINIFSQIGVIV
                     LIGIVTKNGILIVEFANQRKAAGLSIREAVIDAATRRLRPILMTSLATALGALPIAMA
                     LGASSQSRIPMGITIIGGLIFSLILTLFVIPALYTFLSRKIKKTEYEPYAGENE"
     gene            16375..17694
                     /locus_tag="PKZ74_01710"
     CDS             16375..17694
                     /locus_tag="PKZ74_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM91493.1"
                     /translation="MKRWTILLLTSGWVLQGFGQEILTLKDAIRIGLENNYSIIIQKN
                     EAEIAANNNTLGNAGFLPTVNLTGTQNNTITNTHQETFSSGAKDITGATNRTLNAGIQ
                     LTWTLFDGFSMFAGKNMLKIMEEMGETQTRITVENTLSSIIQTYYGIIQQQKLIAVLE
                     DAAALSLERKKIMEARISLGSGSELQFLQATVDLNADSINLIRERSVLQKSMADLNRL
                     LARSPEITFIPADSIKLQTRLNYDSLLAKTRGQNRELQMARNNLSLSNFALKDIRSQR
                     YPRLNFNAGYSYSQLRSETGFLQYNRSLGPSFGVSLSYSLFNGFNVNREIQNARLDLN
                     TMENYLLQTDLSLHTSLYQQYNDYLTNLRIVDMELINREVARENVEVAFAKYRLGTIN
                     DIELRETQKKYIDAQYQLLLSQFQAKNAEIELLRISGELFKVLSVGF"
     gene            17754..18167
                     /locus_tag="PKZ74_01715"
     CDS             17754..18167
                     /locus_tag="PKZ74_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPM91494.1"
                     /translation="MTDDLNQRISKAKQLFDGPYNCSQSVAVAFADILQKDEQEILKL
                     MSGFGFGMGGERSVCGAVSGGIFIISSTADDPAIREEIYDKVFYLVSQFKKLNQGNLH
                     CLDLVGKEPTDEQFRTTCPVLLSQVITLVRKIIKD"
     gene            18380..>21104
                     /locus_tag="PKZ74_01720"
     CDS             18380..>21104
                     /locus_tag="PKZ74_01720"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM91495.1"
                     /translation="MKNILLLFSSIIFLSGFFNSEKVNSQICCPDFILKDAVEICAPH
                     MSCSSDQPGIGFPVAACKNTVHTYTVYPNDLSSFTYTWTVTGGTPSSTNGNPVNILWG
                     NGASGYIKVVITSNNPNFICADSIMQQICLIDGPQAAFTVNYDTVCTGVAISLTNTSL
                     GGSSFTWNMGDGTIYTSPTPPNHTYQSPGTYTIILTVKDMGQGQYVGGPNGENRIPCG
                     CADTALAVVVVLPGIGPEIETDCCYGTVCPGDTSEFCTPMVCGSYNWTVTGGTIISGA
                     GTSCIQVEWDNTYSVPTTVSLQSCPSSSCPGSTTIDVPVLYPNLPISGPNNLCLGASG
                     TFSLPHLPGTFYKWTVSGGPYIFNEKNRNVSLVNISFNSFGTYWVKCEYNNPLAGCDG
                     VDSLLVTVTPKFDIVGPDKICEGDTVFYFTNSLVGGPPANWTFSPAGPVVYTGQGTPN
                     PSIIASPGNYTIIATSLTPPVNCNLVATKNIQVVAKPLLGNINGPTMACPNTNYTYGI
                     TSNTSGSSFIWSVGPGSGAIFSQMGDDNDSVIVQFSGSGPWTLNVYQEIDLGNNDFCK
                     SLTKTLVVNPFPAPNITGAHTVCVDAVENYTASGPVPPGGFQWSISPSAQGTILNGQG
                     SSSVNIKWHGPAATNVVLTVTTCSGQDTIHVAISTPPTVLATPSQPPLFCLGDNQTLV
                     ISTPNIGGYTYQWYDNNGILTGETNSSISLNIASFTNPGTYSYYVVVTNGGCTRTSNI
                     VNVIISTCTGGGGPSGCPPNPVPDCPTVCFSTNVLCGQVQLFNSSYVVPNASIQSYTW
                     SVSPNTGIFTPSNTAPNPTLTVPTSGTYTITLTVISTTNCTASASQAVTVFVPTASFT
                     VSSPVCENSFATFTASPILPGLNYFWTFGDGSTSYSAITQHSYASAALSPYT"
BASE COUNT         5309 a         5408 c         5206 g         5181 t
ORIGIN      
        1 cagttaccgg cttttcgaaa aaactaaaga aggggaattc cccggcgacg gcagggcctc
       61 ctttattttc ctgcccgggg atgaagaggt cctgatcatt ttcgggaacc ggctgattaa
      121 gtggaaaatg gatttgtaaa aatgatattt tatgatgaaa aaaacgacga tttttatcct
      181 gcttgttttt ctggcactcg gattatcagc ccagaaaaaa gccttactgg aagacaaaga
      241 ccagatcatt gaaagggcaa aactggaact ggattcggcg atgatggcac cggagggtta
      301 catttacctt cagacggtta agaatcctat caaaggggaa tacactttcg atatcagcat
      361 ctgggaaaaa ggtaccgtta cttccctttt tgccgtcggc aacaataacg gcacgctgga
      421 agcacaaaac cggctccggg ccatcctcat ggagtttaaa ttcaatttca aaatgcctaa
      481 ggggaaaagc tataagttca ggcatacgtt tgtcttcaat taatatagaa atttcaatct
      541 ttaaccatat taaacatgaa aaaacagatt ctttcatttt tcgcagttct tgcatttctg
      601 agcatttatg cccaggaaga catgaccatg gtgtgggaga caaaatcacc gcacaagatc
      661 accttcaacg gaaccggcac ggaagagcgc ggttacagtt atgtcgcctc cgataaggaa
      721 ttttctttat tcgacaacgc gaccggcaag atcaagtgga ctaaagttta taaagaaatt
      781 gcccctaagc tgagcaagat cgatgaactg atcccgttct gggaatcgaa cacagttttc
      841 ctgttcgacc gcaaaatggg gaaagaccag attgcctgtg tggatatgga aacaggctca
      901 ttgctctgga atacggacaa ataccagaat gttggcgaag aaaatgtagt gtacatcccg
      961 gaacgcgagg gatttgccat ttcattgaaa gacaagctcg ttttcattaa agtcgccacc
     1021 ggggaagaat tatggtcgac ggccaaattc ggcggtgtcg tgggaaaata tgtttttact
     1081 cctgatggtt ttttgaccac ggtcaatttc cagccgtcgg gtctgatcgc ccttttcacc
     1141 ggtttcaaga accagattgc caagatcaat atggacaatg gcgatgtggt atggagcaat
     1201 acctacatcg gccgggcaga gcggaagatc atctcccggg aattcttgta tgatattgcc
     1261 gtggagggcc cccgggtcat cctcaggctg aacggtatcc aggtttacga ttacaacacc
     1321 ggcgccaacc tgtggtcggc cgcattcgac tttaccccca acggcgtcag gtcgaggcct
     1381 cctaatgcca ggcaatgggg cgtttacgga gctgttgccg atccggtatt cgacaaggag
     1441 aacaacgtga tctatgtgct cgatatgtcg aaccgcaaca accaatttgt aaaaaaatac
     1501 gacgccaata ccggcaaact tatctggaca tcacccgaaa tcaaaggggc aaaggcaatt
     1561 cccggtatgt atgttgtgga tgataaggtt atcctgcaaa tcgggggtat cgtcgaattc
     1621 cagtcttatg aggtcataca aacccagtat tctaccatta ccatttggag ggtgttttat
     1681 gataacgtga aacccaacgg attacaagca ttcaacacaa aagacggttc gctggcctgg
     1741 gaatcggaaa aattcaagaa agggatcacc aatgcctatg tgttagagaa caagatcctt
     1801 tgctcgtccg ggaaagaact ttacaagatc gattataaga ccggaaatga agaatattcc
     1861 gttccggtta ccgatggcgg cgtcggactt gccgtgaaga tcctgcctta caaggataag
     1921 atgatcgttg taggtgaaaa aggaatctcc gcctataatc ctgttgacgg taaattcatc
     1981 tacagcggaa aatataaagc ttccagcctg gaggatgtat tcgacaatat cctgatcatg
     2041 aagaccgaaa aagcggatat cgcttcgttc aacctcgata cgggcgtttt caaggagttt
     2101 aaggcgaaga ccggcgcatc gaccaccctg acgaccgatg gtaagtttgt ttatatttat
     2161 gaagataagg tctttaccaa agtaaaaaca ttgtaagaat catatcttat tgattatcat
     2221 attaaaaaaa tcgcaggctg aattgtcaga cctgcgattt tttttacctt cacctcaaaa
     2281 tccccgtcca tgatcaagct aaaagatatc aggccaatcg tccagggtga taaaaaagtc
     2341 atccgtggct gggtgatgta cgactgggcc aattcggtat tccagctgac catcactacc
     2401 gccattttcc ccttgtacta cgatgccatc accaggaacg gcgggagcta tacggtcagc
     2461 tttttcggag ctgaagtcat caatacggtc ctttattcat ggacgatcgg cgcggcattc
     2521 ctgatggtgg cgctgctgtc gccattactc tcgtcgatag cggattatac cggacgtcga
     2581 aaagctttca tgcaggtgta cacctgggtc ggggcgctgg gctgttccat gctgtttttc
     2641 tttaccggcg acaacattga actcgggatc atcgcctttt tctttggcac atggggatat
     2701 gccggcagca tcgttttcta caactctttt ctcccggtta tagcaaagcc ggaagatcat
     2761 gacaagatca gcgcccgggg ctattccatg ggctacctgg gaggcgtcgt gcttttactg
     2821 atgcaactcg tcgtcattca gaaaccttcc tggttcaata tcccggccgg cagcgacctg
     2881 cagttccgcc tggctttcct gactgtaggt atctggtgga taggcttttc gcaaattact
     2941 ttttcaaggc ttcccaagta tgtcttcaaa aaacgacagg ggaaagaaaa tgtcattctg
     3001 aatgggtaca aagaacttcg gactgtttat taccaggtac gaaaatcaaa gaaactgacc
     3061 ctttacctgg tcagttattt ctttttcatg atgggcttgc tgtcggtgat gtatatggcc
     3121 gcctctttcg ggaaaaaaga ggtgaacctc ggcgacgaga tcatgatccc cacactgctg
     3181 ctgatccagc tgatcggtat gggcggggcc tggttctttg cccggctatc cgggaagatc
     3241 ggcaacctga aagcactcat tatcgccctg ataacctgga tcctgatctg ctccggtgct
     3301 tactttatta cagatgctat cggattcata acagcagcat tctttatcgg gatcgttatg
     3361 ggtggaacac aagcgctggc ccggtcaaca tattccaaga tgctcccgga aacgactgac
     3421 cacacctctt ttttcagctt cttcgatgta atggaaaaga tggcttcggc aggcggaact
     3481 ttcagtttcg gacttattga ggccataacc ggtaatatga gggccagtgt ggtagccatc
     3541 gctattttct ttatcatcgg ccttttcttc atcattgtta tgcctaaaag aggaacgcaa
     3601 aacagtgctg agatttgagt tttgagtttt aattgcggga tttgtgctta tctttagcgg
     3661 atgattttaa catccattaa aatgatcttt acgcctaaat tgctcagaaa caccaccatc
     3721 cttattttca tccttctaat tttcaagata acttattcac aaagtcgcca ggataatcca
     3781 gttgtctgga ccggcattac ctccgacaca ccaatggaat cccggatcaa actggtgtct
     3841 tcggatatta cgacgaaccg tatcgaaata atcaccgaag gattcctgaa agaaataatc
     3901 agggataatg aaggtggcaa ggaagttgtc gtctctgctg aaaacaccac tccgcttctc
     3961 atcgaaggcg cccctgacct ggggaaattg agcatccccc tggccatccc cgacctcgac
     4021 cggatgactg tcaccgtcgt ggaggcggat ttcattgact ttcaaaatat cctggtaatc
     4081 ccttcaaaag gaaatctcac ccgcgatatc aatcccgatg acgtccctta tacatacgga
     4141 agggagtacc gcaccgacgc ctttttcccg ggtgttgccg ccgaattgcg cactccgcat
     4201 ataatcaggg atgtcagggc ccaggtggtc gtggcctatc ctttccgcta taatccggtg
     4261 tcgaaagtgc tgcgggtcta tacacgactg gttctggata ttgcctcggc aggtgaagac
     4321 ggggagaatc cttatgtttt aagacgggag gacggcaaga cggtaagaag ctttgaggaa
     4381 atttaccagc ggaagttgct gaattacaag gaataccgga cggcttcgag atataccccg
     4441 cttgaggaag acgggaacat gctgatcatt tgctacggat cattcatggc tgatatgcag
     4501 ccttttgtcg actggaaaaa tatgtccggg atcccgaccg agatggtcaa tgtcagtagt
     4561 gtcgggacaa cggcagcggc aataaaatca tatgtggcca actattacaa caccaatggg
     4621 ctgacatacc tgctgctggt cggcgacgcg gcccaggtcc cggtttcatc caccacggca
     4681 ggaccatcag acaacgacta cggctacatc gtcggcagtg atcattatcc cgacattttt
     4741 gtcggacgat tttctgctga aaccaacgcc cacgttcaga ctcaggttca gcgcacattg
     4801 aattatgaga aacagccgga cctttcgccg ggcttttttg agcgggccat gggcattgcg
     4861 tcggcggagg gccccgggga tgacggggaa tacgactggc agcaccaacg gaatatccgg
     4921 acggattaca tgggattcac ctatacctat ggtgccgaac tttatgatgg cagccagggg
     4981 ggacaggatg ccgcagggaa tccgacagca acaatggttg ccagtgagat caatacgggc
     5041 accgggatca tttcctacac cgggcatggc agcaacactt cctggagcac gacaggtttc
     5101 agtaatacca atatcaccag cctgacaaat accaacaaac tgccctttat cctttctgtg
     5161 gcgtgcgtga atggaaactt tgcaggaata acctgttttg ccgaagcctg gctcagggca
     5221 acctacaaca accagccctc cggcgctgtg gccaccatca tgtcgaccat caaccagagc
     5281 tggaacccgc ctatgcaggg gcaggatgaa atggtggata tcctggttga gaatttcccc
     5341 tcgaatatca agcggacttt cgggggtatc tgcatgaacg gttgcatggg catgaacgac
     5401 gtttacggtt cggcaggtgc agatatgacc gacacctgga ccattttcgg cgatccgtcg
     5461 cttatggtgc gcacagcagc cccggtaaca atagcggttg atcatccggc agatttgccc
     5521 gttggttctg acgagattac ggttttatgc gaccaggaag gagccagggt ggccgtttcc
     5581 cgcgactttg agctcctggg gtccggttat gtttcagggg gcactgcatt ggtggaattt
     5641 ggacaaatca atacggaaga tacgctgatt gtgaccgtta cggcattcaa ttgtatgccg
     5701 tatatcgaca cagtgttcgt aaccaatgcc gagatggttt atgtttccag ccaggctttc
     5761 catggtaacc tcacggaagt ggcccccggt cagcaggata tggaggcgct cggcgtaaaa
     5821 atcctgatgt cgggctccgt aaatccgttc aacctcctta gtctgacatt caacatgaat
     5881 gggacgaccg acctggaaga tgttgaaagt ctggcagttt attataccgg taataacgga
     5941 tcattcggga cggcaacact tttcggtata ggcgtggttt cagcaggatc gatcacggtg
     6001 accgggaatc agcctctcaa cggcggtttc aattatttct ggctggcgta cgacctggct
     6061 gaagatgcgc ttatgggtca ttttctcgac gcggagtgca ccggtatggc cctgggcgat
     6121 aatcctgcac tgacccgggt cccagcaatc accgatcctg acggtcagag ggaaataaac
     6181 cggatgtcgc tggtctcgct cacctgttca cagcccgtaa ccagccctgt tccgcccggt
     6241 acagtcaatt gcgaaatcat ccggcttgag atcacgaccg atggatcgcc tttacccctg
     6301 accgtgacgg gcatcaaggt cgccacccag ggatgtaccg atttggatga tatttccgcg
     6361 ataaaggttt attacaccga tctgccggcg ttttcaacaa caaacctttt cggcagtgta
     6421 gcaccggcca gcttcgttac tgtaaccgga aaccgggcac tgaccgaagg ttccgattat
     6481 ttctggatcg tatttgatat ttcttctgct gccgggccgg gcaatgaact cgacgcagag
     6541 tgcacagaag ttactttcag cggggcgaca ggcacacagg tgccgcttgt caccgatcct
     6601 cccggctacc ggagcatcat gctgaattat tgcaccccca cctatacgta tggatcgggg
     6661 gacggcgatt atatcagcct ggtttcaatc ggatcgcttt cgaaagccac cggggcctct
     6721 tcatcccctt attataatta ttaccaggac gaaacgactc atttagtccc cggaagtact
     6781 taccagctaa cccttagccc gggaacctac ggttcgggga acaacatggc cgcctggatc
     6841 gattataacc ggaacggaac ttttgaatct tccgagaaac tgggcgagct gacgaatatg
     6901 tcgccaacgc ctgcgactgg aactttgact tttactgttc ctgtatctgc aatccatggg
     6961 attctgaggc tgagggtcag agaggtatgg aacaccaccg gactggatcc ttgcgccgga
     7021 tatgattatg gcgaaaccga ggattatgat gtgcacatca tgcccccggg ctgctggctg
     7081 ggttatacca atgaatggac taacgtatcg aactggagcg atggcgtggt ccccgggccc
     7141 ggaacagagg tcatcattcc cgaaatcctc atgggcagct attatccggc tgtttttggc
     7201 aattccaacc tggagatcga acagttgaac atgcaggagg gctcgaacct gagcatcccc
     7261 gtcggcgtta cgatcaccgt tggcggaaac aggaaataat cctgcctcat ctgccgggtt
     7321 tttgctaact tgccccggta tttgaaatat cttcgtgtat ggtggtaaaa attattgttc
     7381 tggctattta tgccctcgtt attataattg tagggattta cggattgaga aaaacgcggt
     7441 ccttcaacga tttcttcctc gggggaggaa aggtgggggc gatcatgagc gccttttcat
     7501 acggtgctac ctatttttca gctgttgttt ttattggttt tgcgggaaaa atcgggtggg
     7561 gattcggcta ttcgggaatc tggataggtg ttttgaacgg attgatagga gttctggccg
     7621 tttgggcgct gctgggctgg agagttaaaa aaatgtcggt tgaaatgggg gtacataccc
     7681 tcagtgaata tcttgaaaaa agatatggaa gtccggcgtt gaaacttatt gcctcaatag
     7741 ccatcttcat tttcctgatc ccctactctg cggccgtttt catcggcctc tcctatcttt
     7801 tcgcctcctc gttcgaaggc ttgcaatact ggcatgccgt gctgttcatg ggcgttttta
     7861 cagctattta cattgttctg ggaggatata agtcgatggc gatgatggat actgtattcg
     7921 ggatcatcat gacgacggga gtgctggttt tgttcggata tacgttaaat gcgggaggag
     7981 gcctggctgc gatcaccgaa aagctgcaat cgatcgatcc cgggctgaca gctacggtag
     8041 gcccgccagg ctggtggccg ctgctttcgc tcatcctgct gacaagcgtg gctccttttg
     8101 ccatgcccca gctcatgcag aagttctatg cgatcaaaga ccggaaagcg atccggaccg
     8161 gcatgatcgc ctcgaccatt ttcgccctgc tgatcggggg tattgcctat ttcctcggtt
     8221 ctaccacgag gattttcctg gcccccgaaa catcgccggc cgcttttact gccgaaggga
     8281 aaccggtttt cgatgcactg atgccggaat tactggcccg tgtaataccg gaatcgttat
     8341 cgatcatcat cctgctcatc atcctggccg cttccatgtc gaccctggct tcgctggtcc
     8401 tgatctccag ttcctcattc tgcaaagatt tttacgccgg gttcatcaac cggaatgtga
     8461 ctgataaaca gcttaccaac ctgatgcgcg gagcaaatct cttctttgta cttctgtcgg
     8521 tcatcctggc cctgatcaaa ccgtcgacca ttgtaaccat cctggccatg tcgtggggtg
     8581 cgatcggttc ggcattcctg ggcccgttca tctggggatt gttctggaaa aggacgacac
     8641 gccttgctgc ttttgcctcg atgctcctcg gcctggggac ctgcattggc ctttcaatcg
     8701 ccggattcgg ggcaccggaa gcgggcacga tcgggatggg cgtttcgctg gccgtgccgc
     8761 ctgtggtgag ttgggcaggc ggtcggcagc ttgtccttag ggcgaagccc gaaggatcgg
     8821 cagtgggcgg tcggtagccg gtatctgccg gagtgtcaga atttcaatcc aaagctggca
     8881 tagatggaag gaagatcttc caatgactgg tagcggaaat taacagaggt cgtgaacatc
     8941 ctggccgctt catattgaat ccctgcaata taataattca gaatatcttc cactcctgca
     9001 gcgttgatct tcagatggtc ccaccggacc agtatatcaa actttccggt aaaattccac
     9061 cctccgaaaa cggaaaaacc gctgtaatcc ttcccattca tccatccgta gttattcaca
     9121 aaatcatatt cgccaccgaa acggaacttt tgcgttttat agcccgcaaa tcccgatatg
     9181 acccatttat cggcataaac agagcctgta tcgacggcgg ggccataatc ggcataaagc
     9241 ttcagggtaa ggtttttgac aggagtatac tggatattgt ttgaaaagat gaattgcccg
     9301 ttgagatcct ggtggcggaa tgggccttcc ccgttgacca ccgaaaactg gttcaggaat
     9361 ttgtctttta cctttacatc caactgtgcc ccgaaatcgg ccggctgacc aagcctgtag
     9421 cgctcctgga aagtaacgtc gatatacctg tatccccaga aaggatcctg gaacgtgagg
     9481 tactgtgtgt tcaaaagctg gccgacgcgc aaagcaatat aatcattgat atcccactta
     9541 atctccgcca ttttcaggta tgccgtgtat ttacttccct caaaataggt aaccgtcatg
     9601 gattggccgg aggaatcctt aatactcgtg atattggtcg tgcgggtaac atcgaacatg
     9661 atcttcccgc tgaccttcgg ggcgatctgg tgaaagtacc cgataatccc ctggttgaat
     9721 tcaaatgccg cctgcggctt gtaagtatcc ttaaatccat acctgaaccc ggtatagatt
     9781 tgtccgaaag catagcgttc cttttccttg ttgagttcca gggtggtttg gctggcagcc
     9841 gttcctgtca tgaaggataa aatcagaaaa aagataatat tgcgcataag aatcagaatg
     9901 acaaaggcaa aattaaccaa gttatagttt ccggaaacgg ttaacaacca ataatataaa
     9961 tataacacga tattacttga catcgtcttg aaaatcacta tatttgttca actaataact
    10021 cattatgtca aacaactaac tgacctgtca tgaagaagca ttacattttt acctgtttca
    10081 ttgccatctt ccttacgctg cccttgctga acctttatgc ccagaaaccc gggatctact
    10141 ttgagccttc atccctcgcc gaaacgctcg cccccgacga ttccgtggtg gtccattccg
    10201 ttctgcacaa cggttcggcc cagacagttg agttctcttt tccgggatac gtgacccgcg
    10261 acctgggggg gccggatgat ttcggctatt cctggatcga tagcgaagag ccgggtggtc
    10321 ccgactggtc atggactgat atttcagaag tgggaatttt agtggaagga ttgggagacg
    10381 atgaaatggc gggaccgttt gagatgggtt tcaacttcag cttctacggc cagaccaaga
    10441 atcatttctg gatcagtccg aatggcgtga tcagcttcaa cgaacagttt attccatatg
    10501 caaacgagcc tattcccacc aatgataatt ataacgattt tatcgcctgg ttctgggacg
    10561 acctgaagat cgattcggcg attacaagag tttattataa aaactataaa gagaaaaccg
    10621 ttgtgcaatt cactaaaatg gtccattatc ccggtaccga atcatttatt accggccagg
    10681 tcatcatgat ggtcaacggt tctatcctat tgaaatacag gctggtcagc gaggattttg
    10741 agacaaccgg agcgacggca ggaatccagt catggaactc ccagcaggga ttgcaggttg
    10801 tgtacaatgc tgaatatgtc cactccgaac tggctgtccg atttgacctg aaccggaatt
    10861 tcatcacctc tgttaatcct tccaccctga cacttccgcc aggaacacag gaaacgatct
    10921 ggatcactta ctcttcgact ggctttgaag tcggatccta tgagcaagac ctgaaatgca
    10981 ttaccagcca tccggaattg cctcacctga tccttcacaa tgtcatgcat gtcaatgttc
    11041 ctgtcaattc aggatttaaa ggatatgtga ccgatgcggc aacaggtttc gccatccatg
    11101 aagtgatggt gaaagcgggt gaacatgtga cctatacgaa cgataatggt tactatgaat
    11161 tacccctgga ggaaggcgtt tataatgtca aattccagcg aaacggatac cagactgtga
    11221 ttgttgaaga cacaacggca ttagagggct attccatact cgatgtcgaa atgagcggat
    11281 attatttcat tgcaggacag gtgtttgcgg atgataacgc gatcggatca ggatttgcgt
    11341 actggtataa acttgtggaa gaagaggttg tgaatatcgg ggccggtatg gtcggggagc
    11401 tgggatggta tgaattcagc agcatgcagg ccggaaacta cattatcaag gctgaaccgg
    11461 ctcccggatc ggattactat gagctttacc tgcctactta ttacggagat gtgctgcatt
    11521 gggaagaagc gacgatcatt gagttgaacc tgagtaccga cgatgcacat atcaaccttg
    11581 tcgccgccac cgttgctaca gaaggaccgg gaacggtttc cggcaagatc gaaagcaata
    11641 cccggactgc cgaaattccc gtattcctcc ggacccttga ccatgctata gtatatatgg
    11701 cggttaccgg aaccgatggc gcctattcct ttaacaacat cccttacgga acctacgaag
    11761 tttttgccga gattccgggg aaatccatca cgcccaaaac aataatcatc gatgaactac
    11821 atactgtgat tgaaaatatt gatatgatga tcatggatac ggagatcatc ttcctgggaa
    11881 tccaggaatc ggatattttc atccatgagc ctttcattta tcccaatcct gccaaagaaa
    11941 cactgaatgt attcaccggg ctgaaaaaga aaacccgtgt ggagataaca atatttgatg
    12001 tagcaggaag aatagttacg gaaagaacac ttgaggcagg agccgatgaa accatccggc
    12061 ttgacgcgtc atttcttcct tcagggcttt acctgctgaa actcgcctca gaaaacgatc
    12121 tccgggttat agagttttta aagtaacggt ttttgggttc tccttccaaa gtaaaataac
    12181 tacttttgcg ggttgttttc gctcaattcg accatatgaa gaatctcaac aggagtaaat
    12241 caatcctagc ctttattata ctcattccga tcttctttgc ttgtaataat aaaggcaatg
    12301 aaccggcaaa acctgctgca gcaaagccgg tggaggtcag agccgttatt gctgaaccga
    12361 aaccggtaga gaataaaatc ttttctacgg gcagcatcct ggccaacgaa gaagtcgaga
    12421 tcagggctga agtccccgga agggttacac ggatcaattt tcaggaagga tcggcggtta
    12481 aaaaagggga tttgctggtt aaaatcaatg acaatgagtt actggcagaa ctgaagaaac
    12541 tcgaactgga tgaaaagctg gcttcggatg acgttttccg gaaagaaaaa ctgatcgaac
    12601 tgaaggcggt aagccaggag gagcttgaca ttgcccgcgg ccagctgggc acaatacagg
    12661 cccagatcga actggtgaaa gcgcggcttg aaaaaaccga aatctttgcc ccctttgcag
    12721 gcaggatcgg tctcagatat atcagcccag gcgggtacat atcgtcagcc atgctgatcg
    12781 cgcgcctgtt gcaaacagac ccggtcaaga tcgaattcac cgtgccggaa aaatatatcg
    12841 gcagtgtgag ccaggggatg gaggttgcct ttaccattgc cggaagcgac agtgtgtttt
    12901 ccggccaggt atatgcgatc gaaccaagga ttgatccatc cacccgctct ttcacggtca
    12961 gggcccggtg ccagaatccc ggcggagtgc ttgtgccggg ggctttcgcc aaggtcacag
    13021 ccattctcga acggataccg gatgccatcg tgctcccctc cgacgccctg attccggata
    13081 tcaaggggga aaaagcctat ctctgccgga acggaaaagc gacagccgtt tatgtcacca
    13141 ccggcatccg caccgaaact gatgtccaga tcacctccgg aatcaacccg ctggatactg
    13201 tcatcacatc cggactgctg cagattcgcg aacagatgag cgtcaccccc cgtatcaacg
    13261 gtaaaaacta aagcggggcc agggttatga acatttcatc tattagcatt aaccgtccgg
    13321 tcctggctac cgtgatctcc atcctgctgg tgctcttcgg cgccatcgga tacacttttc
    13381 tcggcgttcg cgagttcccc agcgtcgatc cgccggtggt cacggtatcc accagttata
    13441 tcggcgccaa tgccgatgtg atcgaatcgc agatcaccga accgttggag gaatccatca
    13501 acggtatcgc cggcatccgt tcactgacat caaccagctc cgacggacga agcaatatca
    13561 cggttgagtt tgaactgggc gttgacatgg aggccgccgc caacgacgtc cgcgaccgcg
    13621 tctcccgggc gatccggagc ctgccgcccg acaccgatcc gcccgttgtg acaaaatcgg
    13681 acgccgacgc cagccccatc ctcgttttga tgcttcagag caacgaacga aacctgatgg
    13741 aactctccga cctggccaac aatgttttta aagaaaggct gcaaacggtt ccgggggtca
    13801 gcgaaatccg catctgggga gaaaagaaat atgccatcaa actgctgatg gatccggcaa
    13861 aactggccag ttaccagctt accccggtcg acgtcaggaa tgcactgacg cgcgaaaatg
    13921 tcgagctccc ctcaggccgt gtcgagggct atcgtacgga actgacaatt agaaccttag
    13981 ggcgtttgac gactcctgaa gagttcaacg acatgatcat ccgggaatct gacggcaccc
    14041 tggttaggtt ccgcgacgtc ggaaaagccg tgttggtacc tgaaaacgaa cgttctatca
    14101 tgcggggcaa caatgcagta ccgctggtag gagtcgccat caccccgcag cccggggcca
    14161 accagatcgc cattgccgat gaatgttaca aacggctgga tcagataaaa ctggacctgc
    14221 cggaagatat ttccgtaggc ctggccatcg acaccactaa gaccatccgt aatgccatca
    14281 cagaggtatt cgaaacattg ctgatcgcct ttggcctcgt ggtcatggtc atctttatct
    14341 ttctccgcac ctggcgtacg accctcatac cggtcatcgc cataccgatc tccctggtca
    14401 gcggattttt tatcatgtat atcgcgggtt tctccatcaa cattctgacc cttctcggga
    14461 ttgtcctggc aacaggcctg gtagtggatg atgccatcgt ggtgctggaa aacatatacc
    14521 ataaggtgga aagcgggatg aacccggtag aagctgggca caagggctcc aaagagatct
    14581 atttcgccat catttccaca acgatcacac tggcggcggt ttttcttccc gtgatcttcc
    14641 tccaggggct taccggacga ttgttccggg agttcggtat ggtcgtggca gggaccatcc
    14701 tcgtttcagc actggtttca ttgaccctga ccccgatgat gagttcccgg atgcttcgga
    14761 aaacggaaaa acacagccgg gttttcaacc ggacggaacg ctggatcgac ggcctgatca
    14821 gcggctataa caaacgtctg acttcattcc ttcgctaccg atggctggcc atcctgatca
    14881 tggtcctttc catcggaatg atcctgggga tcggttccct cgttccgtcg gaactggcac
    14941 ccatggaaga caaaagtcga cttagtattg tatccacagc accggagggg acatccttcg
    15001 aagtcatgga cgaatatatg ctgagtttgc tggaagtggt ggacaccatc cccgaaaaga
    15061 ccaatttcct ggctattacc gcgcccggat ttggttccag tgcctcgacc aataccgggt
    15121 tcttcagggt tatcctgacc gatccttccg aaagggagag gagccagcag gagatagccg
    15181 acgagctcag ccagatcacc cgcaggtaca actttgcccg gagctttgtt acccaggagc
    15241 agacgatcgg cggtggaagg ggcggcgggc tgcctgttca gtttgtcatc ctggcgccaa
    15301 actttgacaa gctcagggcc gttatcccga gattcatgga agaggcccag aaaagcccgt
    15361 tattccaggt aactgacctc aacctgaaat tcaacaagcc ggaggtgagt atcgatatcg
    15421 accggaaccg ggcccgggca ctgggggtga atgtgaggga tgtggcggag aacctgcagc
    15481 tttatttcag cgggcagcga tacggctatt tcatcctcaa caacaaacaa taccaggtga
    15541 tcgggcaggc cgaccgtcta aaccgggatg aaccgctcga cctgagttcg gtctatgtaa
    15601 gaaataaccg caacgagctt atccagctcg ataatatggt cacattgagc gaaaacagca
    15661 atccgccgca gctttaccgc tataaccggt atgtctcggc gaccgtttcg gcgcaacccg
    15721 caaaagggat caccctcggg cagggaattg atgagatgcg caggattgcc ggcgaaaccc
    15781 ttgatgacag tttctcgaca gagcttgcag gtgtatcgaa agaatacgaa gacagttcga
    15841 gcagcctttt atttgctttc ctgctggccc ttgtgctcat ctacctgatc ctggcagccc
    15901 agtttgaaag ctatatcgac ccgctgatca tcatgttcac ggttccgctg gcgctcgcag
    15961 gagcagtgct ttcattgtgg ctgttcggcc agaccatcaa catcttcagc cagatcgggg
    16021 tgatcgtact gatcgggatc gtcaccaaga acggtatcct gatcgtagaa ttcgccaacc
    16081 agcggaaagc ggccgggctg agtatccggg aagcagtgat cgatgcggcg acacgacggc
    16141 tccgcccgat cctgatgacg agcctggcta cagcgctcgg cgcccttccc atcgccatgg
    16201 ccctgggtgc ctcatcccaa agccggatcc cgatgggaat aaccatcatc ggtggcctta
    16261 tcttttcgct gatcctgact ttatttgtta taccggcttt gtatactttt ctttcaagaa
    16321 aaatcaaaaa aaccgaatat gagccttatg ccggtgaaaa tgaataaaaa atgtttgaaa
    16381 agatggacta tcctgttatt gacatcggga tgggtcctcc agggctttgg ccaggaaatc
    16441 ctgaccctga aggatgctat acggatcggg ctggaaaaca attattccat cattatccaa
    16501 aaaaatgagg cggaaattgc cgccaacaat aataccctgg gaaatgccgg gtttctgcca
    16561 actgtgaacc tgacggggac gcaaaacaac acgatcacca acacgcacca ggaaaccttt
    16621 tccagcggtg caaaggatat taccggcgct acgaatcgca ccctcaatgc cgggatccag
    16681 cttacctgga ccttattcga cggcttcagc atgtttgcag gtaaaaatat gctgaagatc
    16741 atggaggaaa tgggagagac acaaaccagg atcacggttg aaaacacact atcatccatc
    16801 attcagacct attacggcat tatccagcag cagaagctga tcgccgtact tgaagatgcc
    16861 gcggctttat cgctggaaag gaaaaagatc atggaggccc ggatcagcct gggatcaggt
    16921 tccgaactgc aattcctgca ggccaccgtg gatttgaacg ccgacagtat caacctgatc
    16981 agggaaagat cggtcctgca aaaatccatg gccgacctga accggttgct ggcaagaagc
    17041 ccggagatca ctttcattcc ggcagacagc attaaactgc aaacccggct gaattacgac
    17101 agcctgcttg ccaaaacccg cggacagaac agggaactgc agatggcccg gaataatctg
    17161 tcactaagca attttgccct gaaagatatc cgttcgcagc gataccccag gctgaatttc
    17221 aatgccggat acagttacag ccagctccgg tcggaaacgg gatttctcca gtacaaccgc
    17281 agcctcgggc cctctttcgg ggtgtcgttg tcgtattcat tgttcaacgg ttttaatgtt
    17341 aaccgggaaa ttcagaatgc ccgcctggat ctgaatacga tggaaaatta cctgctacag
    17401 acggatctca gtcttcatac ttcactttac cagcaatata acgactattt gacaaatctg
    17461 aggattgtcg atatggaact gatcaacagg gaagtggcca gggaaaatgt tgaagtggct
    17521 ttcgcaaagt accggctggg gaccatcaac gatatcgaac tgcgggaaac acagaagaaa
    17581 tatatcgatg cgcaatatca gctcctgtta tcccaattcc aggctaagaa cgcggaaatc
    17641 gaactgctcc ggataagcgg agaactattt aaagtgttga gtgttgggtt ttgagtgctg
    17701 agtttggagt caggagtaag ctggaaatta ctttttaacc ttataacgat actatgacag
    17761 atgatttaaa tcaaaggatt tcaaaagcaa aacagctgtt tgacggtccc tataactgtt
    17821 ctcaatcggt agctgttgcc tttgccgata ttttacaaaa agacgaacaa gagatattaa
    17881 aactgatgag cggtttcgga tttgggatgg gcggcgaacg atcggtctgc ggggcagtat
    17941 ccggtgggat cttcatcatt tcatccaccg ccgatgatcc ggcaattcgg gaggagatat
    18001 atgataaagt cttttacctg gtcagccagt ttaagaaact caaccaggga aatctccatt
    18061 gccttgacct ggtgggcaaa gaaccaaccg atgagcagtt caggaccacc tgcccggtcc
    18121 tgctctccca ggtcatcacc ctcgtccgga aaattatcaa agattagcga cgtaatgcca
    18181 ttacattgtc cattgttcat tgtccattgt ccattgttca ttgtccattg tccattgtcc
    18241 ttaccaacct tacacctctt accacccttt tgttaacttt ctcctaatat tctgttaatg
    18301 ttttgttaat gttttcataa tctgtattat acggatgatg atgaggtact tttgcacact
    18361 tattaaattc cctccggtta tgaaaaacat tctactcctt tttagcagca tcattttcct
    18421 ttcaggattt tttaattccg aaaaggtaaa ttcacagatt tgttgtcccg attttatcct
    18481 taaggatgcc gtcgagatct gcgcgcctca tatgagctgt agcagcgacc agcccggaat
    18541 cggcttcccg gtggcagcct gtaaaaacac agtacacaca tatacggttt atcccaacga
    18601 tctctcttca ttcacctata cctggacagt aacaggcggc acaccttcca gcaccaatgg
    18661 caatcccgtt aacattctct ggggaaacgg cgcctcggga tacattaagg tggtcatcac
    18721 cagtaacaat cccaatttca tctgcgccga ttcgatcatg cagcagatct gcctcatcga
    18781 tggtccgcag gctgctttca ccgtgaatta tgatactgtc tgcaccggag tggccatctc
    18841 gctgaccaat acgtcgctcg gcggcagttc cttcacctgg aacatgggcg atggcacaat
    18901 ctatacctcc ccgacaccgc caaatcacac ctaccagtca cccggaactt atacgatcat
    18961 cctgacggtg aaagacatgg gccagggcca gtacgtcggc ggcccgaacg gagaaaaccg
    19021 gatcccctgc ggctgtgccg acacagcttt ggcggtggta gtcgtgctgc ccggtatcgg
    19081 cccggaaatc gagacggatt gctgttacgg caccgtctgc ccgggggata catcggaatt
    19141 ctgcacgccg atggtctgcg gaagttataa ctggaccgtt accggtggca caatcatttc
    19201 cggcgccggg acctcctgca tccaggttga atgggacaac acctattccg taccgactac
    19261 agtttctttg cagagttgtc cctcctcctc ctgcccggga tcgacaacga tcgatgtgcc
    19321 ggtgctgtac cccaatctgc cgatctccgg tccgaacaat ctgtgcctcg gggcctcagg
    19381 cacattttcc cttcctcatc ttcccggaac attttacaaa tggaccgttt ccggtgggcc
    19441 ttatattttc aacgaaaaaa acaggaatgt ctcactcgtc aacatctcct tcaattcttt
    19501 tggaacctat tgggtgaagt gcgaatacaa caatccgctt gccggttgcg acggggtgga
    19561 ctccttgctt gtcaccgtta caccgaagtt cgacattgtc ggcccagaca agatctgcga
    19621 aggcgacact gtcttctact ttacgaattc ccttgtcggc ggaccaccgg ccaactggac
    19681 cttctcccct gccggccccg ttgtctatac aggccaggga acgcctaatc cttccatcat
    19741 cgcttccccg ggaaactata cgatcattgc gacatccctc actccaccgg ttaactgcaa
    19801 cctggtcgcc actaaaaata tccaggttgt agccaaacct cttctcggaa atatcaatgg
    19861 accaacaatg gcctgcccga ataccaacta cacatacggc ataacctcca atacaagcgg
    19921 cagttccttt atctggtccg tgggtcccgg ttcaggcgcc atattctccc agatgggcga
    19981 cgacaacgat tcggtcatcg tccagtttag cggaagcggc ccctggacac tgaatgttta
    20041 ccaggaaatt gacctcggca acaacgactt ctgcaaatcg ctgaccaaaa cgctggtggt
    20101 gaatcctttc ccggcgccaa atatcacggg ggcacatacg gtttgtgtcg atgctgttga
    20161 aaattatacg gccagcgggc ccgttcctcc aggcggtttc cagtggtcca tatcacccag
    20221 cgcccagggg acgatcttaa acggacaggg aagcagcagc gtcaacatca agtggcacgg
    20281 gcctgctgcc acaaatgtcg tactcacggt gacaacctgc tctgggcagg atacgatcca
    20341 tgtcgctatc agcaccccgc ccaccgtcct ggccacccct agccagcctc cgctgttctg
    20401 cctgggggat aaccagacac tggtcatctc cacacccaat atcggcggtt acacctacca
    20461 gtggtacgac aacaacggga tacttaccgg tgagaccaat tcatccatat cgcttaacat
    20521 cgcgtcattt acaaatcccg gtacctacag ttattatgtc gtggtcacca acggtggctg
    20581 cacccgcact tccaacatcg tgaatgtcat tatatctact tgcacgggtg gcggtggccc
    20641 gtcgggctgc ccgcccaatc ccgtacccga ttgcccgacc gtatgtttta gtaccaacgt
    20701 gctctgcggc caggtccagc ttttcaactc atcgtacgtg gtaccgaatg catcgatcca
    20761 gagctacacc tggtctgtca gcccaaatac gggcatcttc acacccagca acaccgctcc
    20821 gaacccgact ttaacggtgc ccacatccgg gacctacacc atcaccctga cggttatttc
    20881 caccacaaac tgcacggcaa gcgcttcgca ggcggtaacc gttttcgtcc cgacggcaag
    20941 ttttaccgtc tcctcgccgg tgtgtgagaa ctcattcgcc accttcaccg cttcacccat
    21001 tttacccggc ctgaactact tctggacctt cggcgacggc agcacatcct attccgctat
    21061 aacccagcat tcatatgctt ccgccgcttt gtcaccttac accg
//
LOCUS       DAOPNH010000015        20828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2405_length_20828_cov_6.454821, whole genome shotgun sequence.
ACCESSION   DAOPNH010000015 DAOPNH010000000
VERSION     DAOPNH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_20828_cov_6.454821"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PKZ74_01725"
     CDS             complement(<1..1272)
                     /locus_tag="PKZ74_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPM91496.1"
                     /translation="MDRELIINAKSAEVEIALLEDKVLVELQKEKSNQGFQVGDIYLG
                     KVHKIMPGLNAAFVDVGYEKDAFLHYLDLGPQFRSLAKYVKLSIAGKGQKIPIEQFVA
                     EEDIEKTGKITQVLKVNQPVLVQIEKEPISSKGPRISSEISFAGRYLVLVPFSNRISV
                     STRIKSIEERNRLKRLITSIKPKNFGVIIRTVAEGKMVAELDADLKNLVAKWHDMIKK
                     LNEARPPSKVLSELDRTSVILRDVLNESFNSITVNDQAVYEEIRNYIGKIAPKKVDIV
                     KNYKGRIPIFEFFGIDKQIKNAFGKTVSIKTGIYLIIEHTEAMHVIDVNSGHRSRKEN
                     TQEENALSVNLDAAIEIARQLRLRDMGGIIVIDFIDMHESGHRRQLYQKLKDEMAKDH
                     AKHTILPPSKFGLVQITRQRVRPENEIEVLEE"
     gene            complement(1464..1754)
                     /locus_tag="PKZ74_01730"
     CDS             complement(1464..1754)
                     /locus_tag="PKZ74_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM91497.1"
                     /translation="MTKAEIVAEIANKTGIEKVAVQASVEAFMEAVKGSLANGENVYL
                     RGFGSFTIKKRAEKTGRNISRNTTIIIPAHNIPAFKPAKTFVHEVKGNVKVK"
     gene            1891..2976
                     /gene="mutY"
                     /locus_tag="PKZ74_01735"
     CDS             1891..2976
                     /gene="mutY"
                     /locus_tag="PKZ74_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPM91498.1"
                     /translation="MSFGYLIINWYRKNKRDLPWRKTADPYKIWVSEVILQQTRVEQG
                     MSYYHNFTRLFPDAGSLANAPEDAVMKAWQGLGYYSRARHMHEAARKIRDEYGQGFPS
                     TYEELLKMKGIGEYTAAAVSSIAFGEPRPVVDGNVMRVVARYAGIGSAVNTARGKKQI
                     IDFLRSRIDPESPGDFNQAVMELGALICVPRNPRCRDCPVSADCFARIHGKIPELPVL
                     VKAKSTVVIHLNYLVLNQKQDNQTFTFIKKRTGSGIWKNLYDFPLIETVQEIGPEKLS
                     QHEDFVLLMEGSRFSPPREVFSFRHLLSHRELKVKFFTFDVDNFSCKTVLKVPPEELC
                     NYPVPRPIEKFIKKISDRPGIFSENPD"
     gene            3050..3502
                     /locus_tag="PKZ74_01740"
     CDS             3050..3502
                     /locus_tag="PKZ74_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPM91499.1"
                     /translation="MAGVNRVILIGNLGRDPEIRSLEGGVKVANFSLATTETYKGKNG
                     EKVDSTEWHNIVLWRGLAEVAESYLKKGNTVFIEGKIRTREWTDKDGNKRYTTEIVAD
                     NMVMLGGRRDQPAGEYSPPPQRPEKAAPQEPIDNPIDISTEPGDDLPF"
     gene            3702..5045
                     /gene="gldE"
                     /locus_tag="PKZ74_01745"
     CDS             3702..5045
                     /gene="gldE"
                     /locus_tag="PKZ74_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HPM91500.1"
                     /translation="MMIVLEVTTPVRGISIVSGIAAILLLLIGSAMASGAEVAFFSLG
                     PNQIRELRTHGTRANQAVLLLLEKPKRLLATLLIVNNFVNVSIVIISTYLTGILFNFR
                     ESSYWVAFLVQVVAVTSLILLFGEILPRVYATRNNLKFARFLAGPVGLASKIFYPLSS
                     MLIGSTKLIDKRISRKAYNVSINELSDAIELATGADTPQEEKKILRGIVRLGDIEVKE
                     IMKSRVDVTAVDISTGYLELLQKIVGSGYSRIPVYGDSIDQVRGILYIKDLLPHLQEG
                     EDFDWKGLLRDALFVPENKKINDLLQEFQERKIHMAVVVDEYGGMSGIVTLEDIIEEI
                     VGEISDEHDEPLETFEFTQIDDHHYIFEGKTSLNDFCKILDIGDEVFDDVRGESDSLA
                     GLLLELLQRMPAKNEQVSYRQFTFTVKAVDKRRIKRVMVSIDISGSFSDQKTTEN"
     gene            5052..5633
                     /gene="gldD"
                     /locus_tag="PKZ74_01750"
     CDS             5052..5633
                     /gene="gldD"
                     /locus_tag="PKZ74_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPM91501.1"
                     /translation="MKFFLRLTIYNLLIVTFMAGCGEDYTPKPRGYFRIDMPDKEYTR
                     FDSTFPYSFEYPVYAKIVPDTRTTSEPFWMNIDFPQFNGRIHISYKPVEENLEEYLED
                     SRTFVIKHIPKADAIDDSLIYRPESAVYGLVYYIEGPAAASPCQFFITDSTSRFLRGA
                     LYFNFPPNNDSLAPVIDFIENDIRHLITTFRWK"
     gene            5719..>7142
                     /locus_tag="PKZ74_01755"
     CDS             5719..>7142
                     /locus_tag="PKZ74_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPM91502.1"
                     /translation="MKKLTFTLLLLLSVFIVFPQAQYSRVKLFASREEIELLVKEGVG
                     IDHGIFRPGAYTICELSDAELSTVKESGISYEVIIEDVEKFYRERNEPFMDRLSELKE
                     AEYLLSRDWPIPDGFELGSCGGFLTIDQCMEHLDNMAVLYPDLITQRAPLSEMTHNNR
                     ELYWVRLSDNPGTDEEDEPEVLYTGMHHAREGIGMQLLVYYMYYLLENYETDPEVQYI
                     VDNFELYFVPIINPDGYAFNIQNQPAGGGMWRKNRRQNDDGSYGVDVNRNYGYMWGYD
                     NNGSSGEPSDDTYRGPDPFSEPEALNMKYFCEDHDFRIALNYHSYSNLFLYPWGWTEV
                     PCDHDAIFHAFGEIMTKENGYVFGAGSTTIYPTNGGSDDWMYGEQETKSLIYSYTPEV
                     GDGSAGFWPPVSQIIPLCQENMYANIMAARLSGPYATARDLSPSIIEYKTGLIFFEIQ
                     RLGLEDGATYTVSVEPLNDAFASI"
     assembly_gap    7143..7242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7243..8184
                     /locus_tag="PKZ74_01760"
     CDS             <7243..8184
                     /locus_tag="PKZ74_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPM91503.1"
                     /translation="GPFTYTDLEILETESAAFTYTLREDILDGDAVEFLLSVNNGLAT
                     FSDTIRKIYGTPVIIFEDDAESFTKWTSNKWNITTAQYHSPERSIADSPFGQYTNNEN
                     NIVTLVESIDLSDVVYASLGFWAKWDIEADYDYVQVLISDNNGGSWTPVQGKFTRPGS
                     SNQELGEPLYDGVKEDWVREEINLGAYLDKQIKIRFVLRSDTYVTGDGFFWDDMTVTV
                     VDQATGISSDSDLPKEQLISIQPNPSSETVTITLFSDKIDRLMIYNSLGVAVHKVRVA
                     EGDTEIKLDVSGWPSGIYFNALESGSSIVAIEKLVVR"
     gene            8356..9264
                     /locus_tag="PKZ74_01765"
     CDS             8356..9264
                     /locus_tag="PKZ74_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPM91504.1"
                     /translation="MVDTVKIGTLKWHHIVSPSEHDLQYLKDNFHFHPLDIEDCRSTN
                     QRPKIDIYDDYYFIILHFPSFDLHNRFLTIKEVKLFWGEDYVISIDQTNWIVTEMFND
                     ARKLIASKEEIEIGTSDALLYRILEKLMNSSLNLMRRLGLELDRINEDLFSIRAQKTI
                     MQISITRKNIILVNTIFKPQLPLFHKFESGQIAGFAENMEEYWGNILDYNQKMWDMAE
                     DYQELIEGLSKTFDSLQTNRINDIMKTLTFFSAILLPLTFITGLYGMNIGLPLQNDSR
                     SFLIIIGLMMMVVITMLVYFKRRKWL"
     gene            complement(9365..10462)
                     /locus_tag="PKZ74_01770"
     CDS             complement(9365..10462)
                     /locus_tag="PKZ74_01770"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPM91505.1"
                     /translation="MVATIIDHIIPFSGYLPSREYPPVIAGPCSAESERQVLETARQL
                     ARLPEVAAFRCGLWKPRTRPGEFEGVGAEGLAWLRKAKQETGLAIAVEVALPQHIELC
                     LKNEVDILWIGSRTVVNPFSVNEIASALKGVDVRVMVKNPVNPDLLLWIGALERLHQQ
                     GIRKLAAVHRGFSAYHCKPFRNLPQWEIPIELRRLVPDLPVLVDPSHISGNRAMLRPV
                     MQKAADLAFDGLMIESHVDPANALTDGDQQVSPKELADLLARIEWPVNNANEVGREDA
                     GENIRLSIVDLRLSIDEVDGEILRLLAERMELVKKIGARKKELGMDALQLERWTRLVE
                     DRLEKGASLGLSRNFLLKLLHTIHEEALRIQ"
     gene            10587..12044
                     /gene="asnS"
                     /locus_tag="PKZ74_01775"
     CDS             10587..12044
                     /gene="asnS"
                     /locus_tag="PKZ74_01775"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPM91506.1"
                     /translation="MLDHGIKRTKIKELLLTGEIDSEVVAKGWVRTKRESKNVIFITL
                     NDGSTIQNLQVVVQPDTLDASLLARISTGAGLSVRGKLIESPGAGQRVELLANDIILY
                     GEANPDEYPLQPKKHSLEFLREKAHLRFRTNTFSAVTRIRHAMIFAIHKFFTDKGFYN
                     IHTPIITGSDAEGAGEMFRVTILDPTKPPLTETGAVDYKQDFFGKPTNLTVTGQLEGE
                     LAALAMSDIYTFGPTFRAENSNTSRHLAEFWMIEPEMAFYDIHDNMDLAEEMLKYLVR
                     YALDNCREDLDFLAKRLEDEEKNKKKEERTEMGLIDKLEFVLENTFERITYTEAIDIL
                     KNSKPNKEKKFQYPINEWGADMQSEHERYLVEKYFRKPVIITGYPKQIKAFYMKQNDD
                     GTTVAAMDVLFPQIGEIIGGSQREENLDKLYARIRELHIPEETLWWYLETRKFGTVPH
                     AGFGLGFERLMLYITGMGNIRDVIPFPRTPQNAEF"
     gene            12409..13896
                     /gene="rpoN"
                     /locus_tag="PKZ74_01780"
     CDS             12409..13896
                     /gene="rpoN"
                     /locus_tag="PKZ74_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPM91507.1"
                     /translation="MLNQRLQQKLLQKLSPQQILLMKLLQIPTIALEQRIKQEIEENP
                     ALDMPEDGLDNKDDDDFGDVDDGPLGDDDDAETDEFKDKSPEDEFDFNDYLDEGDIPD
                     YRLTVNNQSPDEEQRDIPFAAGTSFQEFLTAQLGLRDVTEKQYQIGLTIIGNLDDAGY
                     LQRDITAMTDDMAFSQNMQVTREEILEVLKVIQDLDPPGVGARNLQECLLLQLRRKED
                     HHPDIETAILILERYFDEFTRKHYEKILKKTRITEPSLKGAIQEILKLNPKPGDSISE
                     TTRQNQYVIPDFSVRNIDGELELTVNSRNAPELRISRTYLDMMETYAGNKHSKGKEAM
                     MFVKQKIDSAKWFIDAIKQRQNTLYVTMKAIMDYQEDYFRTGDETTLRPMILKDIAEK
                     VNLDISTISRVANSKYVETPFGTFLLKTFFSESMQKESGEEVSTREIKKILSDFINAE
                     EKGKPLTDEQLTEILKDKGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            13934..14548
                     /locus_tag="PKZ74_01785"
     CDS             13934..14548
                     /locus_tag="PKZ74_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91508.1"
                     /translation="MEKRLAQVISVVFYPLFVPTYAFAILLTMPAYFSALMPAAAKWM
                     VIGIIFLLTCVIPTLSFILMIKSRLVQSKYLSNRDDRTIPYIVTIIFFYLTFYILKKL
                     QISPVYYYFVIGATMLNVIIMGINFFWKISSHMASVGALAGMTVGLSYFLGTFYFGLI
                     ALSLLISGLTGFARLKLKEHTPAQVYAGFLVGFFTILGLFLVRS"
     gene            complement(14564..15235)
                     /locus_tag="PKZ74_01790"
     CDS             complement(14564..15235)
                     /locus_tag="PKZ74_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM91509.1"
                     /translation="MKNFILAIILLLPFFAVSQEKSKVVGETTIRRVSVGVELFQDFW
                     MNKPEGIDVRAINQGGGAFVMYNAPLGKSPLTFSIGAGLGFHNLYSNSLIDDIKADTI
                     TFSPINDTIDYKRSKLGLTYLDFPIELRLVTKDKFRVAVGVKLGYLLDAKTKYKGENA
                     AGDLVTIKTRKVDQIDKFRFGPTLRIGYDWFQVMGYFSVTQIFEKGLGPEVYPISVGI
                     TLMPF"
     gene            complement(15318..16835)
                     /locus_tag="PKZ74_01795"
     CDS             complement(15318..16835)
                     /locus_tag="PKZ74_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPM91510.1"
                     /translation="MKLKNLKLPASFYNWTSIIGATIALISLFMIVFLFVITSILNQG
                     GSYIGVVIYIALPALLVIGLLMIPIGMIIKYRRNLKKTEKTELRWPAFDFNEPRQRNA
                     FMIFSVGSTIFLLLSAVGSYEAFHYTESVEFCGTLCHEVMKPEYTAYQNSPHARVACV
                     DCHVGAGADWYVRSKLSGLYQVYSVTFDLYPRPIPVPIRNLRPARETCEECHWPEKFY
                     ARQLRIERHYLTDSDNTEWDIHLQMKTGPSLSALGLQEGIHWHINPDISVEYVATSDD
                     REVIPWVRYTNKKTGEVQIYEDASNPLEPGQIDTLEIRQMDCMDCHNRPSHNYQTPIY
                     AINAAITAGKIPRDLPDIKFQAMQVMNATYSTTDSAMMGIEKGIREYYSTVYEELYNS
                     NPEKISQAIDGIREAFSKNIFPEMKVKWSAYPNHIGHLEFNGCFRCHNNNHSTAEGRT
                     ISMDCNLCHSIVAQGTPGDMQVITNLEESLEFFHQTDPDGNWKDALCSDCHRDLY"
     gene            complement(16840..17460)
                     /locus_tag="PKZ74_01800"
     CDS             complement(16840..17460)
                     /locus_tag="PKZ74_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPM91511.1"
                     /translation="MSTVYLYPIWTRLWHLFNALLCLALIFTGLSMQYSNPEYPLIRF
                     DIAVSIHNISGILLSINYLMILIGNLATGNGKYYWIRIKGFYNELLAQGKYYMFGMFR
                     GEKAPYPINVDRKFNPLQKLSYVVIIYFFMPLIIITGWALIYPEMIFVNKIFGTSGLH
                     LTDLLHIIAGFIISLFMLIHVYICTIGKPRGTHFRAIMSGWHQAEH"
     gene            complement(17466..18428)
                     /locus_tag="PKZ74_01805"
     CDS             complement(17466..18428)
                     /locus_tag="PKZ74_01805"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPM91512.1"
                     /translation="MFKNYKYLLFLLICITGHRAPAQEESEIAPITIEKNNECLVCHG
                     YKYFSYFNEYYGRDVKERMNPLYIVDSTEFYLSNHRTFMCTDCHSTEYGDEFPHPNHL
                     RFEPSFSCMDCHEGNDEFARFNFETIRDEFVKSVHSEKHSEDFTCWMCHNPHSYHISA
                     RNSQNLTETIRYDNQICLDCHSDEGRIGLLSDREIFNMLDKHEWLPNHLAHFKSVRCI
                     ECHAEVNDDLMVAHNILPKEKAVKKCVECHSQNSILMASLYKHQVQEKRSSRGYFNAA
                     IMTETYVIGANRNIFLNIASISIFGLAFIGVIIHAILRKIYAKK"
     gene            complement(18637..19551)
                     /locus_tag="PKZ74_01810"
     CDS             complement(18637..19551)
                     /locus_tag="PKZ74_01810"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HPM91513.1"
                     /translation="MKKYILFILVSFSVLSLMAQDTETPKKENKNKSLLGYFILNDSK
                     PLTTYEGGMVIDNQTSYIPSAKTLELLMQHRFGSMNNGLSDFLGIYGAANARMALNYS
                     VTDWLQLGLGTTKNYKLQDLILKVNLLRQSRDKKSPVDVTFYGNYSVDARDESFFGED
                     YQFSDRMAFFNELLVSRQVTKWFTVSAGGSFTHFNQVDSLFEHDKIALHFLGRFKFSS
                     QSAIIVNCDLPLKIDGIKEWLEFTDPPKYNLGIGYEVVTATHVFQIFAGSATSLVPQY
                     NVMLNQNDFFKGTDNIFIGFNISRVWNF"
     gene            complement(19583..19963)
                     /locus_tag="PKZ74_01815"
     CDS             complement(19583..19963)
                     /locus_tag="PKZ74_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91514.1"
                     /translation="MKKILLIVIAITGIMVWQSCQYDWYDPVDYIPPPPGDTTYFSLE
                     IIPIFNNSCNSGSCHGAGGIPPDLSPANAYADLFARNQIDTVTPENSKLYIKCAPGGS
                     MNKYCQPGDPELILQWIKEGALNN"
     gene            complement(20035..>20828)
                     /locus_tag="PKZ74_01820"
     CDS             complement(20035..>20828)
                     /locus_tag="PKZ74_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91515.1"
                     /translation="SKGFREAIANDTTETLAPISNPTQPNCYTCHNIHQTYTESDWAL
                     ATTDPVTFRFNAGNPGMQFDGGNGNLCVNCHQSRTINPYPDINNLTGTVTVGNFRYGP
                     HYGPQSQILSGQAGFNVGITGSTSHKHYEWIENTCTDCHMAEPFGQLAGGHNMSVYIE
                     EEGDYNWAGCTSCHDDEDALATNTEELMTEVTDLEAQLWTLLVNEGIADANNYGYAVP
                     GTYSNLVAGTFLNWKFVHHDRSKGVHNPTYVKALLNNSIEALGGK"
BASE COUNT         5257 a         4927 c         5110 g         5434 t
ORIGIN      
        1 ctcctcgagc acctcgatct cgttctcggg acggacgcgc tgacgggtga tctgtaccag
       61 cccgaacttg ctgggcggca gaatggtgtg cttggcatgg tctttcgcca tctcgtcctt
      121 cagcttctgg taaagctggc gacggtggcc cgattcgtgc atgtcgataa aatcgatcac
      181 gatgatgccg cccatatcgc ggaggcgcaa ctggcgggcg atttcaatag ccgcatccag
      241 gttcaccgac agggcgttct cttcttgggt attttctttg cgcgagcggt gtccgctgtt
      301 cacgtcgatc acgtgcatcg cttcggtgtg ctcgatgatc aggtagatgc cggttttaat
      361 gctcacggtt ttgccgaagg cattcttgat ctgcttgtcg atcccgaaaa attcgaagat
      421 cggaatccgg cctttgtaat tcttgacaat atcgaccttt ttcggggcta ttttcccgat
      481 ataatttcgg atctcctcat acaccgcctg gtcgttgacg gtgatggagt taaacgattc
      541 gttgagcacg tcgcgcagga tgaccgaggt ccggtcgagc tcgctgagca ccttgctggg
      601 gggcctggcc tcgttgagct ttttgatcat gtcgtgccat ttggctacca ggttcttgag
      661 gtcggcgtcg agctctgcta ccattttacc ttcggctacg gttcggataa taaccccgaa
      721 gtttttcggt ttgatgctgg taatcagtct cttgagccgg tttcgctctt cgatcgattt
      781 gattcgggta gagacgctga tgcggtttga aaaaggcacc agcaccaggt aacgacctgc
      841 aaatgatatt tcggatgaga tacggggccc tttggaggag atcggttctt tttcgatctg
      901 caccagcacc ggctggttca ccttgagtac ctgggtgatc ttgcccgtct tttcgatatc
      961 ctcttcggcc acaaactgct ctatagggat tttctgtcct ttccccgcaa tagagagttt
     1021 gacatatttt gccagggacc ggaactgtgg tccgagatca agatagtgga ggaaggcatc
     1081 tttctcatac cccacatcca caaaagcggc attcaacccc ggcatgatct tgtgaacctt
     1141 gccgaggtaa atgtcgccaa cctgaaaacc ctggttgctt ttttctttct gtaattcgac
     1201 aagaaccttg tcttccagta atgctatttc aacttcagct gacttcgcat taataatcag
     1261 ttccctatcc acaatgaagt tgtatttagt atatcatcct gatagccggg tgcagcctgg
     1321 ataataagaa ccggaacata aaggggctaa gattatttct tcttatgcct gttttttctc
     1381 aaacgctttt tgcgtttgtg agttgccatc ttgtgccttt ttcttttctt cccgctgggc
     1441 atcttgtaat gttttaaaaa atgttactta accttaacgt ttcccttcac ttcatggaca
     1501 aaagtcttcg ccggtttgaa agccgggata ttatgtgccg gaataattat cgtggtgttc
     1561 ctggaaatgt tcctgccggt tttttcggcc cttttcttga tggtaaagct accaaaccct
     1621 ctcaggtaga cgttttcgcc attggccaga gagcctttta cagcttccat gaaagcttcc
     1681 acacttgcct gcacggctac tttctcaatc ccggttttgt ttgctatttc agcaacgatt
     1741 tctgccttgg tcataactta attaatttta gatttttaac tttatttttt aaaagaaggc
     1801 tgcaaagata atttattttc gcatgccgga acgcctgatt aaaaaaaaat tggttctaaa
     1861 ctgctcaaaa taaatttgtt tgaagaacgt atgtcatttg gttaccttat aataaactgg
     1921 tatagaaaaa ataaaaggga tttgccctgg cgaaagacgg cggatcctta taagatatgg
     1981 gtttcggaag tcattctcca gcagacccgg gtagagcagg gcatgtcgta ttatcataat
     2041 tttacccgcc ttttccccga tgcaggttct cttgccaatg cgccggaaga tgccgtcatg
     2101 aaagcctggc agggccttgg ttactattcc cgggccaggc atatgcatga agctgcccgt
     2161 aaaatccggg atgaatacgg acaagggttt ccctctacat atgaagaatt attaaagatg
     2221 aaagggatag gggaatatac tgcggctgcc gtttcttcca tcgctttcgg tgaaccgagg
     2281 cccgtggtcg acgggaatgt gatgcgggtg gtggcccggt atgccgggat cggttcggct
     2341 gtgaatactg cccggggaaa aaagcaaatc attgactttc tgagatcaag gattgatccg
     2401 gaaagcccgg gtgattttaa ccaggcagtg atggaactgg gcgctttgat ctgtgttcca
     2461 cgcaacccac ggtgccggga ttgcccggtc agcgctgact gttttgcccg gattcatgga
     2521 aaaatcccgg aattgcctgt cttagttaaa gccaaatcca ctgttgtcat tcatttgaat
     2581 tacctggttc ttaatcaaaa acaggataac cagactttta cctttatcaa aaagaggacc
     2641 ggcagcggta tctggaaaaa tctttatgat tttcctttga ttgagaccgt tcaggagatt
     2701 ggtcctgaaa aactttctca acatgaggat ttcgttttac tgatggaagg ctcccggttc
     2761 agtcctcccc gggaggtgtt ttctttccgc catcttttaa gccatcgaga gttaaaagtc
     2821 aagtttttca cttttgatgt tgataacttt tcgtgtaaaa cggtactgaa agtgcccccg
     2881 gaagagctct gtaactatcc ggttcccagg cctatagaaa aatttattaa aaaaatttcc
     2941 gatcgaccgg gtatattttc cgaaaatccg gattaagcag tagagcgctt ctattatcca
     3001 ttaaatttgt aaaaacaaac cattcattaa ccattaaaaa tttaagatta tggcaggagt
     3061 aaacagggtg attttgatcg ggaatcttgg gagggatcct gagatcagga gtctggaagg
     3121 gggtgttaag gtagcgaact tttcccttgc cacgacagaa acgtacaaag gcaagaacgg
     3181 agagaaggtg gactcgaccg agtggcataa tattgtttta tggcgcgggt tggccgaagt
     3241 ggcggagtcg taccttaaaa aaggcaatac cgtatttatt gaaggcaaga tcaggacgcg
     3301 cgaatggacc gacaaggacg gcaacaagcg ctacaccacc gaaatcgtgg ccgataatat
     3361 ggttatgctc ggcggacggc gcgatcagcc tgccggcgaa tacagccccc caccgcaacg
     3421 gcctgaaaaa gccgcaccgc aggaacccat cgataatccg atcgatataa gtacggaacc
     3481 gggcgacgat ttgccgtttt aggtaagttg gttggcagca ggcagtaggc agtaggcagt
     3541 tttgttttga tggattaata atcgtaaacc aatgactgcc gactgctgac tgctgactgc
     3601 tgactgccga ctgccgactg ccgacttttt cctatctttg ccccgtcaac caattcctga
     3661 gaattttgga aattactgat tttgaacttt cccggagctt tatgatgatt gtgctggagg
     3721 tcacgacccc ggtccgtggc attagtatcg tctccggaat cgctgcaatt ttgttgctgc
     3781 tgatcggttc agccatggcc tcgggcgccg aggtggcttt tttttccctt ggcccgaacc
     3841 agatccgtga gcttagaacc catggcacca gagccaacca ggcggtcctg ctcctcctcg
     3901 aaaaaccgaa aagattatta gctacattgc tgattgtcaa taatttcgtt aatgtttcta
     3961 ttgtcattat ctcgacctat ctgaccggca tcctgttcaa cttccgggaa tcgtcgtatt
     4021 gggtcgcttt cctggtgcag gttgtggcag tgacctcgct gattttgctg ttcggtgaga
     4081 ttcttccgag agtttacgca acacgcaaca acctgaaatt tgcacggttc ctggctggcc
     4141 cggtcggcct ggcgtcgaaa atcttctatc cgctcagctc catgctgatc ggctcaacca
     4201 aactgatcga taagaggatc agccggaagg cttataatgt ttcaataaac gaactttccg
     4261 acgccattga gctcgccaca ggagccgata cgccgcaaga ggagaagaaa atcctccggg
     4321 gtatcgtccg gctcggtgat atcgaggtga aggagatcat gaaatcacgg gtcgatgtca
     4381 ccgccgtcga tatttcgacg ggctatctcg aactccttca aaaaatagtc gggtcaggct
     4441 attccaggat cccggtttat ggcgactcga tcgaccaggt aagggggatt ctttatatca
     4501 aggacctgtt gccacatctt caggagggcg aggatttcga ctggaaaggt ttgctccgtg
     4561 atgctttatt tgtgcccgaa aataaaaaga tcaacgacct gctgcaggaa ttccaggaga
     4621 gaaagataca catggccgtg gtggtcgatg aatacggcgg catgagcggg attgtaaccc
     4681 tggaagacat catcgaggag atcgtagggg agatcagtga tgagcatgat gagcccctgg
     4741 aaaccttcga gtttacccaa atcgatgatc atcattatat tttcgaagga aagacttcgc
     4801 tgaacgactt ctgcaagatt ctcgatatcg gcgatgaagt atttgacgac gtgaggggtg
     4861 agtcggattc actggccggg ttgctgctgg agttattgca gagaatgccc gcgaaaaatg
     4921 aacaggtgtc gtaccggcag ttcactttta ctgttaaggc tgtcgacaag cgcaggatca
     4981 aaagggtaat ggtcagtatc gatatttccg gatcgttctc tgaccaaaaa actaccgaaa
     5041 attaacataa gatgaagttt tttctccgat taacaattta taatttgctg attgtgacct
     5101 tcatggcagg ttgcggggag gattacaccc caaagccaag agggtatttc agaatagata
     5161 tgcctgataa ggagtatacc cggtttgatt caacattccc ttattctttt gaataccccg
     5221 tttatgccaa aattgttccc gatacccgga ctacctccga acccttctgg atgaatatcg
     5281 atttcccgca attcaacggc aggattcata tctcttacaa accggttgag gaaaacctgg
     5341 aagaatatct tgaagattcg cgcacattcg tgatcaaaca tatacccaag gccgacgcta
     5401 tcgatgattc actcatctac aggcccgaat cggctgttta cggcctggtt tattacatcg
     5461 aaggcccggc tgccgcctcg ccctgccagt tttttattac cgatagcacc tcccgcttcc
     5521 tgcgcggggc attgtacttc aatttcccgc caaacaacga ctccctggct cctgtcatcg
     5581 attttattga aaatgatatc cgccacctga tcacaacatt caggtggaaa tgaaatccgg
     5641 gcttgtcatt ctcttgtttt tataaataat tatatttgcg atcatataat attcctgatg
     5701 actcaaatat cttaccagat gaagaaatta acatttaccc tgctgttgtt attgtcggtt
     5761 tttatcgtat tcccccaggc tcagtattcc cgtgtgaaat tatttgcctc ccgggaagag
     5821 atcgaattgc ttgtaaaaga aggagtgggg attgaccatg gcattttcag gcccggagcc
     5881 tacacgatct gtgagctgtc ggatgccgaa ctttccactg taaaggaatc tggaatttca
     5941 tatgaggtaa ttattgaaga tgtggaaaaa ttttaccgtg agagaaacga gccttttatg
     6001 gacaggctca gcgaactgaa ggaggcagaa tatctcctca gccgcgactg gccgattccg
     6061 gatggttttg aactgggctc atgcggaggt tttctcacca ttgaccaatg catggagcat
     6121 ctggacaata tggctgtttt gtatccggat cttattacac agcgggcccc tctcagcgag
     6181 atgacgcaca ataaccgcga actatactgg gtcaggctct ccgataatcc cggtacggat
     6241 gaagaagacg agccggaggt tttatatacc ggtatgcacc atgcccgcga aggcataggg
     6301 atgcagttgc tcgtttatta tatgtattat cttctggaga attatgaaac cgatccggag
     6361 gtgcaatata ttgtcgataa ttttgagctt tattttgttc cgatcatcaa tccggacggt
     6421 tatgctttca atattcagaa ccagccggcc ggcggaggta tgtggagaaa gaaccggagg
     6481 cagaatgacg atggctctta cggcgtggat gtgaacagga actacggtta tatgtggggt
     6541 tacgacaata acggctcatc aggtgaacct tccgatgaca cataccgcgg gcctgatcct
     6601 ttctcggagc ctgaggcgtt gaatatgaag tatttctgtg aagaccatga tttcaggata
     6661 gcattgaatt atcattcgta cagcaatctt tttctttatc catggggttg gacggaagtg
     6721 ccatgcgatc acgatgccat ctttcatgcc ttcggtgaaa tcatgacaaa agagaacggg
     6781 tacgtgttcg gcgccggcag caccacgatc tatcccacca atggcgggtc cgacgactgg
     6841 atgtacggtg agcaggagac aaaatccctc atttattcct acacacctga ggttggcgat
     6901 ggaagtgccg gtttctggcc gcccgtgtcg cagatcatac cgctatgcca ggagaacatg
     6961 tatgccaata tcatggcggc aaggctgtca gggccctatg ccactgcccg cgatctttct
     7021 ccatcaatca ttgaatataa aaccggcctg atcttctttg agattcaacg actgggcctg
     7081 gaagacggtg caacctacac cgtttcggta gaaccgctca atgatgcctt tgcatccatc
     7141 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggccgtt tacgtatacc
     7261 gatctggaga tccttgaaac agaaagcgcg gcattcactt atacactgcg cgaagatatc
     7321 ctggacggag atgccgtcga attcctcctt tcggtcaata acggcctggc aaccttttcc
     7381 gatactatcc ggaagatcta cggcacaccg gtaatcattt tcgaagatga tgcggaatct
     7441 tttaccaaat ggacctccaa caagtggaac atcactacag cccagtacca ttctccggaa
     7501 cgctcaattg ccgactcccc gtttggtcag tacaccaaca atgaaaataa tatcgttacc
     7561 cttgtggaat ccattgatct gtcggatgtt gtatatgcca gcctggggtt ctgggccaaa
     7621 tgggacatcg aagcggatta cgattatgtt caggtactga tctccgataa taacggagga
     7681 tcatggactc cggtccaggg aaagttcacc cgccccggca gttccaacca ggaactgggc
     7741 gaaccccttt acgacggggt gaaagaagat tgggtcaggg aagagataaa cctgggcgct
     7801 tacctcgata aacagattaa gatacggttt gtattgcgaa gcgatacgta tgtgactggc
     7861 gatggtttct tctgggacga tatgacggta actgtggtcg accaggcaac cggcatcagt
     7921 tccgattcag atctgccaaa agaacaactc atcagcatcc agccgaatcc atcatcagaa
     7981 acagtgacca tcaccctgtt ttctgataag atcgacagac tgatgattta taattcgctg
     8041 ggtgtggccg ttcataaagt acgcgttgct gaaggagata cggagataaa gcttgatgtt
     8101 agcggctggc cttcaggcat ttatttcaac gctcttgaat cgggaagcag tattgtggcc
     8161 atagaaaaac tggtcgtcag gtaattggcg gctcaacttg tgaatccgcc ggaaaacacg
     8221 ttttcctgaa ccattcattt ttctccgaaa aagtcaatcc tttccggaac ctttgtttat
     8281 ctttgcaccg cttttaaatt ttacaggcat gtagaacaat tcgcatagta ttcatcttat
     8341 aaaaccggag cagatatggt tgatacggta aaaatcggta ccctgaagtg gcaccacatc
     8401 gtaagcccgt cggagcacga cctgcagtac ctgaaagaca acttccattt ccatcccctg
     8461 gacattgaag actgccgaag taccaaccag cgccccaaga tcgatatcta tgacgactat
     8521 tatttcatca ttcttcattt tcccagcttc gacctgcaca accgtttcct gaccatcaaa
     8581 gaggtgaaac ttttctgggg tgaagactac gtgatcagca tcgaccagac gaactggata
     8641 gtgaccgaga tgttcaacga tgcccgcaaa ctgattgcca gcaaggaaga gattgagatc
     8701 gggacctccg atgcgctgct ttaccgtatc cttgaaaagc tgatgaattc atcgctcaac
     8761 ctgatgcggc ggctgggcct ggagctcgac cggatcaacg aagacctttt cagtatccgg
     8821 gcccagaaaa ccattatgca gatctccatc acccggaaaa acatcatcct ggtcaatacc
     8881 atatttaaac cacaacttcc gctattccac aaattcgagt cggggcagat cgccggattt
     8941 gccgagaaca tggaggagta ctggggcaat atcctcgact acaaccagaa gatgtgggat
     9001 atggccgaag actaccagga gctgatcgaa gggttgtcga aaaccttcga ttcgcttcag
     9061 accaaccgga tcaacgacat catgaagacg ctgacctttt tctcggcgat ccttctccca
     9121 ttaaccttta tcaccggcct ttacgggatg aacatcgggc tgccgctgca gaacgactcc
     9181 cgctcttttc tgatcattat cgggctgatg atgatggtgg tgatcacgat gctggtgtac
     9241 ttcaaaagga gaaaatggct ctaaagaaat tttgaatgtt gaattttaaa ttttgaattc
     9301 ggggatttat taatatttcg tggatcttgg tgacctcttc gtagcacttc gtcaaaagtc
     9361 tgatttactg tatccgcaac gcctcttcat ggatcgtatg taacaatttc agaaggaaat
     9421 tgcggcttaa accaagggaa gccccttttt ccagccggtc ttccaccagc ctcgtccacc
     9481 gctccagttg caaagcgtcc attcccagtt cctttttccg cgcaccgatc tttttcacca
     9541 attccattct ctcagccagc aatcttaata tctctccatc cacctcatca atcgacaatc
     9601 gtaaatcgac aatcgacaat cgaatattct ctcccgcatc ttcccttccg acttcattag
     9661 cattattgac cggccattca atacgcgcca acaaatctgc cagttccttc gggctgacct
     9721 gctgatcgcc gtcggtaagg gcattggccg gatcgacatg cgattcgatc atcagcccgt
     9781 cgaaagccag atcggctgct ttctgcatca caggacggag catggcccgg ttcccgctga
     9841 tatggctggg atcgaccagc accggcagat cggggaccag cctgcgaagt tcgatcggga
     9901 tctcccattg cggcaggttg cggaacggtt tgcaatggta ggcggagaag ccccggtgca
     9961 ctgctgccag tttgcggatg ccctgctggt gcaaccgttc cagcgctccg atccatagca
    10021 gcaggtcggg attgaccggg tttttcacca ttacccgaac atcgacacct tttaaggcag
    10081 aggctatctc attgaccgag aatggattga ctaccgtacg ggaaccgatc cagagaatat
    10141 cgacctcatt tttcaaacat aattcgatgt gttgaggcag cgccacttcc acggcaatcg
    10201 ccaatccggt ctcctgtttt gcttttcgga gccaggccag tccttccgcc ccaactccct
    10261 cgaattcccc gggccttgtc cggggtttcc acaggccgca tcggaaagcg gcgacctcgg
    10321 gcagcctggc cagttgccga gcggtttcca gcacctgtct ctccgattcg gcgctgcacg
    10381 ggccggcaat aacgggcgga tattcccggg aagggagata accggagaaa gggatgatat
    10441 gatcaataat tgttgccacc atgcaaaggt acttttttca ggatttgaca tattaatcga
    10501 ttcggagttg tttgttttca gcaattgaac aatagaacaa tagagcaatc attttcttat
    10561 ttttgcacct caaaatcaag aagaacatgc tcgatcacgg aattaaaagg acgaagatca
    10621 aagaactgct cctgacggga gaaatcgact cggaggttgt ggccaaggga tgggttcgca
    10681 ccaagcgcga aagcaagaat gtcattttta tcaccctcaa cgacggatct acgatccaga
    10741 atttgcaggt tgtggtgcag cccgacacgc tggacgcttc gttgctggcc aggatcagca
    10801 ccggcgccgg gctgtcggtg cggggaaagc tgatcgaatc gccgggagcg ggccagcggg
    10861 tcgagttgct agctaacgat atcattctct acggcgaggc caatcccgac gagtacccgc
    10921 tgcagcccaa gaaacattcg ctggagttcc tccgtgaaaa agcccatctc cgcttccgga
    10981 ccaacacctt ctcggctgtc acccgcatcc gccatgcgat gatcttcgcc atccataaat
    11041 tcttcaccga caaaggattt tacaatatcc atacgccgat catcaccggc tccgatgccg
    11101 aaggggcagg ggagatgttc cgggtgacga tcctcgaccc cacgaagcct ccgctgaccg
    11161 agaccggcgc cgtcgattac aaacaggatt ttttcggcaa acccacgaat ctcaccgtta
    11221 ccggacagct cgagggggaa ctggccgcac tggccatgtc ggatatttac accttcgggc
    11281 cgactttccg tgccgagaac tccaatacca gccgccacct ggccgagttc tggatgatcg
    11341 aacccgagat ggctttctat gacatccacg ataatatgga cctggccgag gagatgctga
    11401 agtacctggt gagatacgcc ctcgacaatt gccgcgaaga cctggatttc ctggcgaaaa
    11461 ggctggaaga tgaggagaag aataagaaaa aagaggagcg gaccgaaatg ggccttatcg
    11521 ataagctgga atttgtgctg gagaacactt ttgagcggat cacctatacg gaggctatcg
    11581 acattctaaa gaattccaag cccaataaag agaaaaagtt ccagtacccg atcaacgagt
    11641 ggggggccga catgcaatcg gagcacgaac gctacctggt ggagaaatat ttccgcaagc
    11701 cggtcataat caccggttac ccgaagcaga tcaaggcctt ctacatgaag cagaacgacg
    11761 atggcaccac ggtggcggcg atggatgttt tgtttccgca gatcggcgag atcatcggcg
    11821 gttcgcagcg cgaggagaac ctggataagt tatatgcccg gatcagggag ctgcatatcc
    11881 cggaagagac gctatggtgg tacctggaaa cgcgtaaatt cggcaccgtc ccgcatgccg
    11941 gtttcggcct gggctttgag cgcctgatgc tgtatatcac cggtatgggc aacatccgcg
    12001 acgtcatccc ctttccccgg acgccacaga acgcagagtt ttagtcgttg gtcaattttt
    12061 cttgaggctg tctcaaaagg attaaaccac aaagggacac caaggaattc acaaaggcgc
    12121 acaaaggtat gttaattgat attcaactac ttagtgttcc ttcgtgatgt actttgtgcg
    12181 ccttagtggt taataaaatg ccttttgaga cagcctcgga atagttggca gtccgacgac
    12241 taaagccgtc ggttgaaatt tgaaatgctc cgacaggtaa atcctcatat ccccaaatcg
    12301 tcaaatcttc aaatcgttaa atcgttaaat cgtcaaatct tcaaatcttc aaatcgtcga
    12361 atcttattat ttttgtatct ttaaataaaa tttggaaccg gcatccctat gttaaaccag
    12421 cgtctacagc agaagttgct ccagaagctc tctccgcagc agatcctgtt aatgaagctt
    12481 ctccagattc ctacgattgc gctcgaacag cgcatcaagc aggaaatcga ggagaacccg
    12541 gcattggata tgcccgaaga cggcctggac aataaggacg atgacgattt tggcgatgtg
    12601 gacgacggcc cgctgggcga tgacgatgat gccgaaacgg atgaatttaa ggataaaagc
    12661 ccggaagatg aattcgattt caacgattat ctcgatgaag gcgacatccc cgattaccgg
    12721 ctgacggtca acaaccaaag cccggacgaa gaacagcggg atatcccgtt cgctgccggt
    12781 acctctttcc aggagttcct gactgctcag ctcggcctgc gcgatgtcac cgagaaacaa
    12841 taccagatcg ggctgaccat catcggcaac ctcgacgatg ccggatacct gcagcgggat
    12901 atcaccgcta tgaccgacga catggccttc tcccaaaata tgcaggtaac ccgtgaggaa
    12961 atcctggagg tgctgaaagt gatccaggac ctcgacccac cgggcgtcgg cgcccggaac
    13021 ctgcaggaat gcctccttct ccagttgcgc cggaaagaag accaccatcc cgatattgaa
    13081 accgctatcc tgatcctgga gcggtatttc gacgagttca cacgcaaaca ctatgagaaa
    13141 atcctgaaaa agacccgcat aaccgagccg tcgctgaagg gcgccataca ggaaatcctc
    13201 aaactgaacc ctaaacccgg cgattcgatc agcgaaacaa cacggcaaaa tcaatatgtc
    13261 atccccgatt tctccgtccg caatatcgat ggcgaactgg agctgacggt caattcccgg
    13321 aatgcccccg aattgcggat cagccggacc tacctcgata tgatggagac atacgccggc
    13381 aacaagcaca gtaaagggaa agaggccatg atgttcgtca agcagaagat cgattcggca
    13441 aaatggttca tcgatgccat caagcaaagg cagaataccc tctatgtcac gatgaaagcg
    13501 atcatggatt accaggagga ctacttccgg acaggcgatg agactacgct caggcctatg
    13561 atcctgaaag atatagctga aaaggtgaac ctcgacatat ccacaatctc ccgtgtagct
    13621 aacagcaaat acgttgaaac gccatttggt acttttcttc tgaaaacatt cttttcagaa
    13681 tcaatgcaga aagaatcggg tgaagaggtt tcgacacgcg agatcaagaa aatcttatcg
    13741 gattttatca atgcggaaga gaagggcaag ccgcttacgg atgaacagct taccgagata
    13801 ctgaaagaca aggggtataa cattgcgcgc cgtaccgttg ccaaataccg cgaacagctt
    13861 aatattcccg ttgcccgcct gaggaaggag ctctaacgaa aaaagcctta tttttgcccc
    13921 tgaaatttca ccgatggaaa aacgcttagc ccaggttata tccgttgttt tttacccgtt
    13981 gttcgtgccg acgtatgcct ttgccatcct gctcaccatg ccggcttatt tctcggcgtt
    14041 gatgccggcg gcggccaaat ggatggttat cgggatcata ttcctgctca cctgcgttat
    14101 tcccacactc tcgtttatcc tcatgatcaa atcgcgcctg gtccaatcaa aatatctctc
    14161 aaaccgcgat gaccggacca taccttatat cgttaccatc atcttctttt atctcacctt
    14221 ctatattctg aaaaaattgc agatatcacc cgtgtattac tattttgtca tcggggcgac
    14281 tatgctgaac gtcataatca tgggaatcaa tttcttctgg aagatcagca gccacatggc
    14341 ttctgtcggg gccctggccg gtatgacggt cgggttatct tattttctcg ggacttttta
    14401 cttcggattg attgccctgt cgttgttaat ctccggcctg accggatttg cccggctgaa
    14461 actgaaagag catacgcctg cccaggttta tgcaggattc ctggtcggat tttttactat
    14521 cttaggatta ttcctggtca ggagttgaag aattaatctc ttttcagaaa ggcatcaggg
    14581 ttatacccac cgagatggga tatacttccg gcccgagccc tttttcaaag atttgagtaa
    14641 cggaaaaata acccatcacc tggaaccagt cgtaaccgat ccgtaatgtc gggccaaaac
    14701 ggaacttatc gatctggtcg actttccgtg tcttgatcgt caccaggtcg ccggcagcat
    14761 tttcaccttt gtacttggtc ttggcatcga ggaggtaacc cagcttaaca cccacggcca
    14821 cgcggaattt atctttggtt acaaggcgga gttcgatcgg aaagtcgagg taagtcagcc
    14881 caagcttgct gcgtttgtaa tcgatagtat cattgattgg ggaaaatgtg atcgtgtcgg
    14941 ctttaatgtc atcgatcagg ctgttactgt aaagattatg aaacccaagg ccggcgccga
    15001 tggaaaaggt cagcgggctt ttgcccagag gggcattgta catcacaaaa gctccgcctc
    15061 cctggttgat cgcccgtacg tcgatgcctt ccggtttgtt catccagaaa tcctggaaca
    15121 gttcaacgcc gacactgaca cgccgaatgg tggtttcgcc gacgacttta cttttttcct
    15181 gtgagacagc aaaaaatgga aggagaagaa taatggctaa gatgaaattt ttcatgaccg
    15241 gaattccgtt taattgatca ggcaaagata aagcttttaa acggaaatta tgcggtgttt
    15301 agtatgatag caacagatca gtaaagatcg cggtggcaat cggaacacag tgcatctttc
    15361 cagttgccgt cggggtcggt ctgatgaaaa aattccagcg attcttcgag gttggtgatg
    15421 acctgcatat cgcccggagt cccttgtgcg acaatcgagt ggcacaggtt acaatccatg
    15481 gaaattgttc ttccttcggc agtagagtga ttgttattat gacaacggaa acagccgttg
    15541 aactcaagat ggccgatatg gttgggatag gcgctccatt tcactttcat ctccggaaag
    15601 atatttttgc taaaggcttc ccggatccca tcgatggcct ggctgatctt ttccgggttt
    15661 gagttgtaca gctcttcata gaccgttgaa taatattccc ggatcccttt ttcgatgccc
    15721 atcatagccg agtcggtcgt ggaataggtt gcgttcatga cctgcatggc ctggaacttg
    15781 atatcgggca gatccctggg gatctttccg gctgtgatgg cggcatttat ggcatagatc
    15841 ggggtctggt aattgtgtga aggccggttg tggcaatcca tgcaatccat ctgcctgatt
    15901 tccagtgtat caatctgtcc gggttccaat ggattcgacg cgtcttcata aatttgcacc
    15961 tccccggttt tcttatttgt gtagcggacc cagggaataa cctcacggtc gtcgcttgta
    16021 gccacatatt ccacgctgat atcgggatta atgtgccagt ggataccttc ctgcaatccg
    16081 agggcgctca ggctcgggcc tgtcttcatc tgcaggtgaa tatcccattc ggtattatcg
    16141 ctatcggtaa ggtaatgacg ttcaattctc agctgccggg cgtagaattt ctccggccag
    16201 tggcattcct cgcaggtttc cctggccggg cgaaggttac ggatcggaac gggaataggc
    16261 cgagggtaaa ggtcgaaggt cacggaatag acctggtaca atcccgaaag tttggaccgg
    16321 acataccagt cggcgccggc accaacgtgg caatcgacac aagccacgcg ggcatgcggc
    16381 gaattctggt acgcggtata ttccggtttc atcacttcat ggcacagggt gccgcagaat
    16441 tccaccgatt cggtataatg aaaagcttca taactcccta cggctgagag tagaagaaag
    16501 atggtcgatc cgaccgaaaa gatcatgaat gcattccgct gccggggttc gttgaaatcg
    16561 aaagcgggcc agcgtaattc ggttttttcg gtttttttca gatttcgcct gtatttgatg
    16621 atcattccga tggggatcat caggagaccg ataaccagca gggccggcag ggcgatgtaa
    16681 atcaccacgc caatgtatga ccctccctgg tttaagatcg atgtaatgac aaacaggaaa
    16741 acgatcatga aaaggctgat cagggcgata gtggccccga tgatcgaagt ccagttgtag
    16801 aacgaggccg gaagtttcag gtttttaagt ttcatggctt taatgttcgg cttgatgcca
    16861 tccgctcatt atagcacgga aatgggtgcc ccttggttta ccgatcgtgc agatatagac
    16921 atggatcagc ataaacaatg agatgatgaa acctgcaata atgtgcaaaa ggtcggtaag
    16981 gtgaaggccg ctggtgccga aaatcttgtt cacaaatatc atttccgggt agatcagcgc
    17041 ccagccggta ataatgatca aaggcataaa gaaatatata atcactacat aactaagttt
    17101 ctgaagcggg ttgaattttc tgtcgacatt gatagggtac ggggcttttt cacctcgaaa
    17161 catcccgaac atgtaatatt ttccctgggc gagcaattca ttataaaatc ctttgatcct
    17221 gatccagtaa tattttccgt tgcctgttgc caggtttccg atgaggatca tcaggtaatt
    17281 aatagataag agaataccac tgatattatg gatgctgacc gcaatatcaa agcggatcaa
    17341 cgggtactcg ggattggaat attgcatgct caggcctgta aagatcaggg cgaggcagag
    17401 gagtgcattg aaaaggtgcc agaggcgtgt ccagataggg tagaggtata cagtcgacat
    17461 ttcagttatt ttttagcgta gatttttctc aggattgcat gaatgatcac cccgatgaaa
    17521 gccagcccga agatgctgat actggcaata ttcaggaaga tgttccggtt ggcaccaatg
    17581 acgtaagttt ctgtcataat tgcggcgttg aaataccccc gtgaagatct cttttcctgc
    17641 acctggtgtt tgtaaagcga tgccatcagg atggagttct gggaatggca ctcgacacat
    17701 tttttaaccg ctttttcttt tgggaggata ttatgggcaa ccatgaggtc atcattcact
    17761 tcggcatggc attcaatgca ccggacactt ttaaaatggg ccaggtgatt gggaagccat
    17821 tcgtgtttat cgagcatatt aaaaatttcc cggtcggaga gcaggccgat ccggccttca
    17881 tcggagtggc aatccaggca gatctggttg tcgtaccgga tagtctcggt gaggttctgg
    17941 ctgttccggg cactgatatg atatgagtgc ggattgtggc acatccagca ggtaaaatct
    18001 tcgctgtgtt tctccgaatg aacacttttc acaaattcat cccggatagt ctcaaaatta
    18061 aaccgggcga attcatcgtt gccttcgtgg caatccatac acgaaaaact gggctcaaag
    18121 cgcaggtggt tcggatgcgg aaactcatcc ccgtattctg tggaatggca atcggtgcac
    18181 atgaaggtac ggtggttcga caggtagaat tccgtggaat cgacgatata gagcggattc
    18241 attctttcct tcacatccct cccgtaatat tcattgaaat aggaaaagta tttatatccg
    18301 tggcaaacaa ggcattcgtt atttttttca atggtgatag gtgcaatttc agattcttcc
    18361 tgtgccggag cccgatggcc ggtaatgcag atgagcagga agagcaggta tttatagttt
    18421 ttaaacattt ataaatcgtt aaagtggttt atacagtcaa tttagaaggt gtctcataca
    18481 ccttctattt aaccacaaag gcgcacaaag tactcacgac ggaacacaaa atatttaatt
    18541 ataaaagtag tgatctttgt gttcctttgt gtaatccttt gtgttccttt gtggttttat
    18601 ctttttttat acaaactcta aaaaaatcaa acctcatcaa aaattccaga cacgggaaat
    18661 attaaacccg ataaaaatat tgtcggtccc tttgaaaaaa tcattctggt tgagcatgac
    18721 attatactgc ggaacgagtg aagtcgctga tccggcgaag atctggaaaa cgtgggttgc
    18781 ggtaacaacc tcgtagccga ttcccaggtt atatttcggg gggtcggtaa attcaagcca
    18841 ttccttgatg ccgtcgattt tcagcgggag gtcacaattc actataatag ccgattggga
    18901 gctgaatttg aaccgtccga gaaaatggag ggcgattttg tcatgttcaa agagcgaatc
    18961 gacctggttg aaatgggtaa agcttccccc ggccgaaacg gtgaaccatt ttgttacctg
    19021 cctggaaacc agcaattcat tgaagaaagc cattctgtcg gagaactggt aatcttcgcc
    19081 gaagaaactt tcgtcgcggg catcaacgga gtagtttcca tagaaggtca cgtcgacggg
    19141 cgatttctta tccctgcttt gccgcaggag attgactttc aggatcaggt cctggagttt
    19201 gtaatttttg gtggtgccca ggcccagttg cagccagtcg gtaacggaat agttaagcgc
    19261 catgcgtgca ttggcagcgc cgtatatccc gagaaaatcg gagagcccgt tattcatcga
    19321 accaaaacgg tgttgcatca gcaactccag tgttttggcc gaaggtatgt aactggtctg
    19381 gttatcgatc accattccgc cttcataggt agtcaggggc ttgctgtcat ttaaaataaa
    19441 gtagcccagc aggctcttgt tcttgttttc cttcttgggt gtttcagtat cttgagccat
    19501 taaagaaagg acactaaaac tgaccaggat gaaaagaata tactttttca tgttcattcg
    19561 gttttgaatt tttgaattcc ggttaattat tcaaagcgcc ttcttttatc cactgaagga
    19621 taagctcagg atctccgggc tggcaatatt tgttcatgga gccacccgga gcgcatttga
    19681 tgtacaattt gctgttttcc ggtgtaacgg tgtcgatctg gttccttgcg aacaggtcgg
    19741 cgtaggcatt ggccggtgaa aggtccggcg ggatgcctcc ggcgccgtgg caactgcccg
    19801 aattacagct attgttaaag atcgggataa tttcaagcga gaaataagtg gtgtcgccgg
    19861 gtggtggtgg gatgtaatcc accggatcat accagtcgta ctggcacgac tgccaaacca
    19921 tgatccccgt aatagcgatg acgatcagta atatcttttt catatagtta tgttttttca
    19981 aagttaaaaa aaaggcttca tatttgaagc cttttatatc atatttaatg aatattattt
    20041 accgccgaga gcttcaatag agttgtttaa taaagcttta acgtaggttg gattgtgaac
    20101 ccccttgcta cggtcgtgat ggacaaactt ccagttcagg aaggtgccgg ctaccaggtt
    20161 ggaataggtt cccggtacgg cataaccata attgttggca tcggcaatac cttcgttgac
    20221 caggagggtc cagagctggg cttccaggtc ggtcacttcg gtcatcagtt cttcggtatt
    20281 cgttgcaaga gcgtcttcgt cgtcatggca agaggtgcaa ccggcccagt tgtagtcacc
    20341 ttcttcttcg atataaacac tcatattatg tcctccggcc agttgtccga acggttcggc
    20401 catatggcaa tcggtacagg tgttctcgat ccactcataa tgtttatgag acgttgaccc
    20461 ggtgatgccg acattgaaac cggcctggcc tgaaaggatc tgcgactgcg ggccgtagtg
    20521 agggccataa cggaaattcc ccacggtaac cgttccggtc aggttgttaa tgtccggata
    20581 cggattgatg gtacgtgact ggtgacagtt tacacagagg ttgccgtttc caccgtcgaa
    20641 ttgcatcccc ggattaccgg cgttgaacct gaaggtgaca gggtcagtgg tagccagtgc
    20701 ccagtcggat tcggtgtagg tttggtggat gttgtgacag gtgtagcagt ttggctgtgt
    20761 cggattcgaa atcggagcca atgtttctgt ggtatcgttg gcaatggctt cccggaatcc
    20821 tttgcttg
//
LOCUS       DAOPNH010000016        20629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2440_length_20629_cov_6.295519, whole genome shotgun sequence.
ACCESSION   DAOPNH010000016 DAOPNH010000000
VERSION     DAOPNH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2440_length_20629_cov_6.295519"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKZ74_01825"
     CDS             <1..861
                     /locus_tag="PKZ74_01825"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPM91516.1"
                     /translation="NLNQASFSYWTAGGTNSLAFSALGKYSANYKQNKFSWNNNLNLM
                     YGMVKNQGESLKKSEDLIELISVAGLDLNPKWSVTGYMSFRTQFANAWDKDNDSIKVS
                     QLLSPGYLTLSPSVRFKPNDNFYVLISPVTAKFTFVTNQDLADKGSFGVTPAEYDSVG
                     NQWVKVTDGDNMLLYLGPFLEAYYKKTVVKNLTYETRFNVLYTLLNRDNLEGYDADVS
                     WENYLNYSIAKYFNISLLLHLVYLPGQPAIKFDNYEGAVRVKAIPNRHIQIKETIGFG
                     ISYIFPAEKK"
     gene            861..1685
                     /locus_tag="PKZ74_01830"
     CDS             861..1685
                     /locus_tag="PKZ74_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPM91517.1"
                     /translation="MDEGLNIDELQEKSRLVRTDILRSLAEAGSGHTGGSLGLTDLFV
                     SLYFNILNHKPGVPDWDGRDRLILSIGHVAPVLYATLAHAGYFPKDELLTLRRLGSRL
                     QGHPGREHGLPGLELSAGSLGQGLSVAVGLALAAKADKKSWRVYSVHGDGEMQEGSIW
                     EAAMSAAHYKLDNLIAYIDRNGVQIDGRTGDVMEIEPLADKWRAFGWEVFECDGHDHR
                     RIIETTRNARRIISKPSVIILKTIMGKGVRSIEGDYRWHGKAPSKEELERFLRELA"
     gene            complement(1669..2745)
                     /gene="mnmH"
                     /locus_tag="PKZ74_01835"
     CDS             complement(1669..2745)
                     /gene="mnmH"
                     /locus_tag="PKZ74_01835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPM91518.1"
                     /translation="MKKYPVVGKNIFKALGSRPLGRHPLGFRPGGRHPLGFRPGGLID
                     VRSPAEYQQGHIPGAFNLPLFDDRERAAVGTAYTRQGKDAALHLGLELAGPKLTYFVQ
                     KAGEIAQGEEILVHCWRGGMRSEAMAWLLNFAGMKATILPGGYKAYRRSVAELFKNDF
                     RFIVLGGMTGCGKTELIQWLHSIGEQVIDLEKLARHKGSAFGALGQPEQPTQEQFEND
                     LADCLEKLDPGQTIWIEDESRNIGKVIIPGAIYEAVCRSEVVLIERPFVERVNRLLNE
                     YGYFENRELSEIIIKISKRIGGDVAQAALKELESGEVRGAIEIILKYYDKTYAYGLSK
                     RPDDNITRISIEDFKNLAFIRPAP"
     gene            2780..3430
                     /locus_tag="PKZ74_01840"
     CDS             2780..3430
                     /locus_tag="PKZ74_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91519.1"
                     /translation="MPSKMIKTKVLPEKYYHIFNQAINNEFLFREDDDYQFFLLKIKE
                     LIIDYFELYAYCLLPNHFHILLKPLWEVNSIHDSSVNEKLRKLFQLYAQYYNKKYKRK
                     GSLYLKPFRRIEIDTEAYLTYLVFYIHYNPQKHGIIGDYRAYDRSSYKFFICDQETKL
                     QRDVVLKWFGDNPEDFVRFHQECLERKIGDGTPSLGMPSQGMASPFFLPLPKFQPL"
     gene            3427..4272
                     /gene="nfo"
                     /locus_tag="PKZ74_01845"
     CDS             3427..4272
                     /gene="nfo"
                     /locus_tag="PKZ74_01845"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPM91520.1"
                     /translation="MKYVGAHVSAAGGVENAPLNAYKIGAKAFAFFTKNQKQWKTAPL
                     SEKNIREFKANCLKYNYLPESILPHDGYLINPGSPDKEMLEKSREALLDEMQRCEQLG
                     LKMLNFHPGAHLGKISEEDCIKIIAESINIVLSKTSDVTAVIENTAGQGSAIGHRFEH
                     LRAIIDQVDDKSRVGVCLDTCHAYAAGYDLKNPEGYDKAFDEFGKIIGFNYLRGFHLN
                     DARKGLASRVDRHESLGKGDIGEDLFIRIMNDPRFDNMPIILETPNEEIWPEEIRWLY
                     ETVGI"
     gene            complement(4312..5133)
                     /locus_tag="PKZ74_01850"
     CDS             complement(4312..5133)
                     /locus_tag="PKZ74_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPM91521.1"
                     /translation="MCGRYVLVQKVELIEKRFNVEAPQGFDFQPNYNIVPGSYAPVIT
                     NENPRQLELFRFGLTPFWSKKPLLLINARAEGDHNPDDNPEYTGARGIITKPSFRKPI
                     RSQRCLIPADCFIEGTTKEKLSRPYVLYLREGERPFAFAGVWDTWANPDTGEIIRSFA
                     IITTVANELLRKIPHHRSPVILPRSLEKAWLSDDLPLSDVTGMLRPYPAELMNAYPVD
                     PAIKSPRAAGPQLLKPVGQRLTAEFDVRKSQDVNLQGMGMNKRLKDQQDYLEFEL"
     gene            complement(5146..6066)
                     /locus_tag="PKZ74_01855"
     CDS             complement(5146..6066)
                     /locus_tag="PKZ74_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91522.1"
                     /translation="MVRSGWRTIRNYFIILLYFLAIPLQGQNLTDLLPNPAELPGWTL
                     QSQTVMNGDELYMLINGGADIYHEYGFSRVVSALYSDPGQNVIRAEIYEMTDDGAAYG
                     VYSINHLKASWTGEFGKQSTVTDDFIAFWKSRYYVILSWKSKKNGGKPLLGQLAAIID
                     GKVSTSGDIPGILGLCSAQASSSNKAYLRGNIGLSNFYYIDYRNPFAFKEAMVFSRED
                     YHVILLEYTNSTESFENMQSARQSLSENKRFTDLTNTYQGFSCKDNKGNRIVVRQENR
                     YLILLVGLSPDAPLPAKMEEISLEIDNLNH"
     gene            complement(6127..7065)
                     /locus_tag="PKZ74_01860"
     CDS             complement(6127..7065)
                     /locus_tag="PKZ74_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM91523.1"
                     /translation="MDRRRFIYKGSGLAAGSLMLNMVPFRLFSGSLKPDLCKVKSGDY
                     YKATRKAIEELGGIGRFIPAGGARVGLLINSAFDEPGAYVHPDIPMAVLYLCWEAGAS
                     EMTLLQPVAEEYWKRSPQFSKHRFIINELKQVESNVFPAVYNEEDFNLMPIIPGAKQL
                     KDVEIIRKIADIDVFINIPILKHHASTILTGALKNMMGLTTRKTNVTFHLGSGKRNDP
                     EYLAQCIADLNLVRKPDLTVADATEFITGNGPAGPGPMKKLDLVVASTDPVAVDAFGA
                     TCLDAAPADILTVYMAYEHGLGEMNLEKLAVAEFEV"
     gene            complement(7076..8068)
                     /locus_tag="PKZ74_01865"
     CDS             complement(7076..8068)
                     /locus_tag="PKZ74_01865"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPM91524.1"
                     /translation="MKALKIIGIILLILIALFLVVALFLPSKSYMEESIVINKPASLV
                     YKQVNDFRNWEAWSPFQAADPQMVNTYEGAEKGVGATMRWTSKKSGNGFMTITESVPY
                     EKVISTLDFGMPGTTNIFLLKEENGATTVNWAVDIQKMPYPMGRYMAVMMPGMMKPVF
                     KQGLDKMKEVVEAMPDPPALQIVEFPSKAVISVVDSCYWSDIEAKMGQLFGELMAVQQ
                     SSKAQQTGAPLSIYHKWDEESQFTVFENCIPVDREVKSKGRVQYKVLPATRAVMGTHF
                     GPYENTMYLYIALDEYVKDFKLTETGGPIEEYITDPMMEPDTSKWQTNVYFQVQ"
     gene            8242..11532
                     /locus_tag="PKZ74_01870"
     CDS             8242..11532
                     /locus_tag="PKZ74_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPM91525.1"
                     /translation="MSKVLFTLYFSLLIAIAGSGQETGVLTGVLTDQYTGDPLFGVKV
                     RVWEQGNAEAQKSSVFVALSDEDGIFSLELPAGQYQASFSRYGLDKLEIPGIMVLAND
                     TAILNTTLLPVEVAVAGVLVNTEPVYLEIQWTPSWPSIIEKSIDYGPVNDYFVFMNAG
                     SQVAVKFDNNFDHVIGGRIFVGDGSLPGPFLGSDFLLRIYDDDGMGGLPGNTLFEDTV
                     TVDQYGWVGLDSLDAFTSSDHYYLSMYQLNEAPECAPVVSTQYPANSSSLMKPGSYNW
                     MPFPPGNAMIRAWVSAPHDSIHVSSYRVGRFSNFDPNGDPQYGTLTELASTNNCFYND
                     NAFPGLPQGWYAYGIKALYNNGQYSPYRISGIVYNPDPCNPSFTIEQSDGSVSGTCHI
                     SMTGISLPFKNYSFLVQTPGTINLSPIKMGSYSISLYKPGYEKWMIDSVGLFSDTSIN
                     ITLQETIYPIEDLKVNEYTGMLDWENDFVSQYEWQCPADSICHELITPELDLLGLNQW
                     IMTVTYMTPLDNDPPILEYSTDHGRTWALLHEFPLQENWTTVDIDLVVFPNESEGSGI
                     MFRLHDVYGAPYYTDISRIKVWLPSVRCHPEKYYITLDEMPEGQSDSTFYQSDNLENG
                     QSYRAGVFAEYSTGTTDTAFVEFTYHELFPPEDLAWEEENDTLEFSWSVPSGSWNNDK
                     APGDYPGALSGYALTYQTPDNLYRFEIDGPATTSFRFEKPDCDSATAILTAVYDLSDY
                     GYPGSLFESKPAGPLQIKFDGPVENEFFEDWSAISLIHNCWKMTGNGLSIEAGQGFPG
                     AALIYQNPPSHYQVFLTSVPINIPESEDAQRFLECDLTLFAGGYSGYEIMEIHLQQAG
                     SSYWDVVQEFSNSSGSYDWSHYSLDLSGYISSDEFRVRFKFEGIGAEPVQWKVDNIHL
                     HRVCSGPTTLQAELTGENEVSLNWLPNTATKSATSFEHYNIFRKTGQDGFSLLATTTD
                     TFYLDYLKEGGRYSYRVNAAYNDNGILCTSSPSDSAWIVSTYDIPENDIHGRISVYPN
                     PVNERLFIVSDEMIYGINLYNSLGICVLDMDKSGLNFEIDMKSLANGIYFLRIELENK
                     DYNAKIIH"
     gene            complement(11622..11966)
                     /gene="rplT"
                     /locus_tag="PKZ74_01875"
     CDS             complement(11622..11966)
                     /gene="rplT"
                     /locus_tag="PKZ74_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPM91526.1"
                     /translation="MPRSVNAVASKARKKKVLERVKGQWGSRKNVWTVAKNAYEKSLA
                     YAYVGRKQKKRDYRALWIQRINAAVREHDMSYSVFMGKLKEHNIEINRKVLADLAMHN
                     PEAFKAIVDKVK"
     gene            complement(12042..12233)
                     /gene="rpmI"
                     /locus_tag="PKZ74_01880"
     CDS             complement(12042..12233)
                     /gene="rpmI"
                     /locus_tag="PKZ74_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPM91527.1"
                     /translation="MPKMKSKSGAKKRFAETGTGKIRRKHAYKSHILTKKSNKRKRNL
                     GHFTEVSGADLKNVKDMLQ"
     assembly_gap    12263..12362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12400..12960)
                     /gene="infC"
                     /locus_tag="PKZ74_01885"
     CDS             complement(12400..12960)
                     /gene="infC"
                     /locus_tag="PKZ74_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPM91528.1"
                     /translation="MATSLPFKGRPPFRDNRAKEEPYKVNELIKAPVVRVVGENIQTG
                     IYNIREALRIAEELELDLVEISPTANPPVCKVVDYKKFLYEKKKKQKEIKANSAKIVI
                     KEIRLSPNIDDHDFNFKLKHAIKFLEEGAKVKVDVFFKGRSIIYKEQGEYVLLKFAAD
                     LEEFGKVEQLPKLEGKRMMMVIAPKK"
     gene            complement(13008..14948)
                     /gene="thrS"
                     /locus_tag="PKZ74_01890"
     CDS             complement(13008..14948)
                     /gene="thrS"
                     /locus_tag="PKZ74_01890"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPM91529.1"
                     /translation="MINITFPDHSVRQFAEGVTGMEIAMSISEGLARNVLAAKVNGEV
                     WDATRPIENDASLQLLTWNDTEGKATVWHSSAHLMAEAIEQLYPGVKFGIGPDIENGF
                     YYDIDFLDYKITEEDLKKIEERMLALAREKQSFKRREVKKSEAVEYFTRKGDQYKLEL
                     IEGLNDGEITFYESGTFTDLCRGPHLPDTGVIKAVKLLNIAGAYWRGDEKRQMLTRIY
                     GITFPKQKELDEYLLLLEEAKKRDHRNLGRDLELFTFSQKVGAGLPLWLPKGAELRER
                     LEQFLKRVQRRAGYVQVITPHIGNVELYRTSGHLAKYGKDSFQPINTPIEGEQFFLKP
                     MNCPHHCEIYKSKPKSYKDLPLRVAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCT
                     PEQLKDEFKGVIDIVLLIFKALDFKDYITQISLRDPANTEKYIGSDENWEKAQNAIIE
                     VAKEAGLNYEIVEGEAAFYGPKMDFMVRDALGRKWQLGTIQVDYNLPERFEMEYTGSD
                     NEKHRPIMIHRAPFGSMERFVAVLLEHCAGNFPLWLTPEQVIVLPISEKFTEYGKKVI
                     TLLQNSDIRALIDERSEKAGRKIRDAEIRKIPYMLIVGEKEEEGGTVSVRRHGQGDMG
                     ALPVEEFINLVKNEVEESLKHL"
     gene            complement(15049..16140)
                     /gene="thyA"
                     /locus_tag="PKZ74_01895"
     CDS             complement(15049..16140)
                     /gene="thyA"
                     /locus_tag="PKZ74_01895"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPM91530.1"
                     /translation="MAQQYLDHSKAILTSPRSNFKGGSKGSGIISLFGYQNEYDLREG
                     YPLLTTKKMATRSMFHETIWFLRGDTNIKYLEDNNCPIWRPDSFQDNLPGMQKAGIFP
                     EGLVKYSPDWDKAMEEYGQRIREDEEFAARWGDAGPIYGKQWRRWEYFDHEKGAFIEI
                     DQLGKMIAGLKKKPTGKKHIVDSWNPGDTPRMSLPPCHVLYQATANEEGELELQLYQR
                     SCDQFLGVPFNIASYAALTQIIAQEADMVPKTFIHTFGDAHFYTSIGKRTQWYKANFK
                     EVQQKIRNVSQREDYLDVIRWIDKNAPSDGNEEKYDHVTAILEQMSREPRPLPRLRIA
                     KKPFDQLTVDDFVIEDYDPHPPIRRNMCV"
     gene            complement(16300..16857)
                     /locus_tag="PKZ74_01900"
     CDS             complement(16300..16857)
                     /locus_tag="PKZ74_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM91531.1"
                     /translation="MPDRLLFIDTETGGLDPDKHSLLTLALVVWEDRNIIDSLEIAVN
                     DGINCVTQEALSINKIDIGKHLQTALSLPEAMDQVMSFTGKHFPGKEKITLAGHNVHF
                     DAGFLKKFFSANHIDFSEIFSHRLIDTSSILHYLYLAGRIEKKAISSDDAFALFDIEV
                     EGRHTALGDATATVKLFSKLLDLMG"
     gene            complement(16906..17139)
                     /locus_tag="PKZ74_01905"
     CDS             complement(16906..17139)
                     /locus_tag="PKZ74_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPM91532.1"
                     /translation="MPDDPVTEIFSGTLWEAELLQTMLNDNEIDCFLKNSTLNTFVFE
                     PTFAGGVKVMVLQSNLVKATEIVNEFRKNIEKA"
     gene            complement(17193..17927)
                     /locus_tag="PKZ74_01910"
     CDS             complement(17193..17927)
                     /locus_tag="PKZ74_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPM91533.1"
                     /translation="MKIAILSDIHGNSYALSKVLEDIRARNADAIINLGDSLYGPLNP
                     SGTWEMLNACHAINISGNEDRIILENLTKDSGNKTLEYVKSQIDNRMTDWLRSLPFDM
                     IFNDLVYGCHASPLSDTEYLLEKVSENHIDIRSQMELDELLKEKTQKIIVCGHSHVPN
                     IVQTDCKLIINPGSVGLPAYDDDLPVYHRMQNYSPRANYSILNVNGESVSVERVALEY
                     DFESAARLAERNGRNDWAHWIRTGIV"
     gene            complement(17945..18751)
                     /gene="zupT"
                     /locus_tag="PKZ74_01915"
     CDS             complement(17945..18751)
                     /gene="zupT"
                     /locus_tag="PKZ74_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPM91534.1"
                     /translation="MTTENLLFAFGLTFFAGLATGIGSLMSFLSKKFNPGFLAGSLGF
                     SAGVMIYVSLVEIFAKAKVSLVAAHGLKAGNIYTVVAFFAGIALIALIDKLIPSYENP
                     HEIKNIDEKNLDPDKSRKLLRMGMFSALAIAIHNFPEGLATFMGALTDPTLGISIAVA
                     IAIHNIPEGIAVSVPVYYATKNRKKAFWLSFLSGLAEPVGAFIGYFILRSVFNDSTFG
                     VIFAGVAGIMVYISLDELLPTAEEYGKHHIAIGGLVAGMAVMAVSLLLFI"
     gene            complement(18795..19736)
                     /locus_tag="PKZ74_01920"
     CDS             complement(18795..19736)
                     /locus_tag="PKZ74_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPM91535.1"
                     /translation="MKRIIIISILTATLLGCSKEKKSDLAAEKMQAFVSDISAYARNY
                     DPGFIIIPQNGIELAFNNLEPGDGINTSYLSAIDGFGVEELFYNGPYTPDNERIAMLQ
                     RLKDYKKILVSEYISDSDNIPDALSRNYTEGFICFPRISSNYHYHLIPDSVPHPNAFD
                     ITNLPDAQNYLYLISTDSFASKQVMIDEISATDFDIVLIDLFFNETEFTSSEINLLKR
                     KANGAQRLVISYVNIGSAENFRYYWKPDWELHHPHWLKKKYEGYDDEIWVEFWEKEWQ
                     DIIFGNDHSYLKKILDAGFDGAYLDNVEAYYLLYFCH"
     gene            complement(19789..20199)
                     /locus_tag="PKZ74_01925"
     CDS             complement(19789..20199)
                     /locus_tag="PKZ74_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPM91536.1"
                     /translation="MKRRVFVDTDIIYDLLGKRDPFYLASAQLFTWADEGKIQIYVSA
                     LSLANLHYLISKQRTENQAKEILRKFKVLVHVTPLNDKIIDLALNSEFADFEDAIQYY
                     SALQNEIEVFLTRNLKDYKKAQITVLTAQDYINL"
     gene            complement(20196..20441)
                     /locus_tag="PKZ74_01930"
     CDS             complement(20196..20441)
                     /locus_tag="PKZ74_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HPM91537.1"
                     /translation="METKLTLRLKKKVIDQAKKYADDHDTSLSKLIENYLSAITSESK
                     PVEKISPLVLSISGVITISENDDPKGNYHKHLQEKYL"
BASE COUNT         5240 a         4978 c         4718 g         5593 t
ORIGIN      
        1 aatcttaacc aggcatcttt tagttactgg acggccgggg ggacgaattc gcttgctttt
       61 tcggccttag gaaagtattc ggccaattac aaacaaaaca aattttcctg gaacaacaat
      121 ctcaacctga tgtacggcat ggtgaagaac cagggggaaa gccttaagaa gagcgaagac
      181 ctgatcgaac tgatttcggt cgccggtctg gatctgaatc caaaatggtc ggttacaggg
      241 tatatgtcgt tcagaacgca gtttgccaat gcatgggaca aagacaatga ttccataaaa
      301 gtttcccagc tcctttctcc cggttacctg acattatctc cttcagtacg attcaaacca
      361 aatgacaatt tttacgttct tatatcaccg gtaactgcca aattcacttt tgtgaccaat
      421 caggatctgg ccgataaagg ctcttttggt gtgacaccgg cagaatatga ttctgttgga
      481 aaccagtggg tcaaagtgac ggacggcgat aatatgttgt tatacctcgg cccttttctg
      541 gaagcatatt acaagaagac tgtcgtaaag aatcttacgt atgagacccg tttcaatgtt
      601 ctttacacat tgcttaacag ggacaatctc gaaggatacg atgccgatgt aagctgggaa
      661 aattacctga attattcgat cgcaaaatat ttcaatatca gcttgttgct gcacctggta
      721 tatttgcccg gccagcctgc gatcaagttc gacaattacg aaggggccgt cagggtgaaa
      781 gccattccta accgtcacat ccagatcaaa gagaccatcg gcttcggtat ttcgtatatt
      841 tttccggccg aaaagaaata atggatgaag gattgaatat agatgagctt caggagaagt
      901 cacgcctggt ccgtaccgat attcttcgta gcctggccga agccgggagc gggcatacag
      961 gcggatcgct gggattgacc gatctgtttg tctcgctgta tttcaacatt ttgaatcata
     1021 agcctggcgt gcccgactgg gatgggcgcg acaggcttat cctttccatt gggcatgtag
     1081 cgccggtttt gtatgctact ctggcccatg ccggttattt tcccaaggat gagttattga
     1141 ctctgcgcag gcttggctca aggctgcagg ggcatccggg gcgggagcac ggtttgcctg
     1201 ggctggagct ttcggccgga tcgctgggac aaggattgtc ggtggcagtc ggcctggccc
     1261 tggcagcgaa agcagataaa aagagctggc gtgtttacag tgttcacggg gatggcgaaa
     1321 tgcaggaagg atcgatctgg gaagcggcca tgagcgccgc gcattacaag ctcgacaacc
     1381 tgatcgctta catcgaccgg aatggggttc agatcgacgg caggaccgga gacgtcatgg
     1441 agattgagcc attggcggat aaatggcgtg ctttcggatg ggaggtattt gagtgcgatg
     1501 gccacgatca ccgccgcatc attgaaacta cgcgaaatgc ccgcaggatc atttccaaac
     1561 cttccgtaat catcctgaaa acgattatgg gcaagggcgt caggtccatc gaaggcgatt
     1621 accggtggca tgggaaggcg ccatcaaaag aggaactgga gcggtttctc agggagctgg
     1681 cctgataaac gccaaattct taaaatcttc aatgcttatt cgggtaatat tatcatctgg
     1741 ccttttagac aggccgtatg catacgtttt atcgtaatat ttcaggataa tttcaatcgc
     1801 tccgcggact tcgcctgatt ccagctcttt cagcgccgcc tgtgccacat caccgcctat
     1861 ccttttcgat attttgataa tgatttcgga tagttcccgg ttttcaaaat agccatattc
     1921 attcagcaac ctgttgactc gttctacgaa tggcctctcg atcagcacga cttctgaccg
     1981 gcacacagct tcataaattg caccgggaat gattactttc ccgatattgc ggctctcgtc
     2041 ctcaatccaa atcgtttgtc cggggtccag tttctcgagg cagtcggcca ggtcattctc
     2101 aaactgttcc tgcgtcggtt gttccggctg gccgagtgcg ccgaatgccg atcctttatg
     2161 ccgggccagt ttttccaggt cgatgacctg ctctcctatt gaatggagcc attggatcag
     2221 ctctgttttc ccgcatcctg tcataccgcc caggacaata aaccgaaaat cgtttttgaa
     2281 caattcggca acggaccgcc gataggcttt atatccaccc gggaggatgg tcgctttcat
     2341 gccggcaaag ttcagcagcc aggccatggc ttcacttcgc atcccgccgc gccagcaatg
     2401 gacaaggatt tcttctccct gagcaatttc accagctttt tgcacgaagt aagtcagttt
     2461 tggtccggct agttcaaggc cgagatggag tgctgcatct tttccctggc gggtgtatgc
     2521 ggtgccgacg gcggcacgct ccctgtcgtc gaacagggga aggttgaagg cgccggggat
     2581 atggccttgc tggtattctg ccggggagcg gacgtcgata agtcccccgg gacgaaatcc
     2641 caagggatgg cgtcccccgg gacgaaaccc caaaggatgg cgtcccaaag gacggcttcc
     2701 caaggcttta aaaatatttt tccccaccac cgggtatttt ttcatcaaaa accggattaa
     2761 ttaataaaaa tataggatta tgccatcaaa aatgattaaa acaaaagttt tgcctgaaaa
     2821 atattatcat atttttaacc aggccatcaa caacgaattc ctcttcaggg aagatgatga
     2881 ttaccaattc tttctgctca agatcaaaga attgataatt gactacttcg aactgtacgc
     2941 ttactgttta ttgcccaatc actttcatat actactcaaa ccattatggg aagtaaacag
     3001 tatacatgat tcatcagtga atgaaaagct tcgaaagttg tttcaacttt acgcacaata
     3061 ttacaataaa aagtacaaaa gaaaagggag cttatattta aaacctttca ggagaataga
     3121 aattgatacg gaggcatatc tcacctattt ggtcttctat atccactata atcctcaaaa
     3181 acacggtatt atcggagact atagggcata cgatcgtagc tcttataaat tctttatctg
     3241 cgatcaggag acaaaacttc agcgggatgt ggttttaaaa tggtttgggg acaatccgga
     3301 agatttcgta aggtttcatc aggaatgtct ggaaaggaag atcggagacg ggacgccatc
     3361 ccttgggatg ccatcccaag ggatggcgtc cccttttttc ctacctttgc caaaattcca
     3421 acccctatga aatacgtcgg tgctcatgtc agcgcagcag gtggcgtcga aaatgccccg
     3481 ctcaacgctt ataaaatagg tgcgaaagcc tttgccttct tcaccaaaaa ccaaaaacag
     3541 tggaaaaccg caccactatc agaaaaaaac atccgggagt tcaaggctaa ctgcctgaaa
     3601 tataattatc tacctgaaag catactgccg cacgacggct acctcatcaa tcccggaagc
     3661 cccgacaaag agatgctcga aaaatcacgc gaagcccttc tggatgaaat gcaacgctgc
     3721 gaacagctcg gcctcaaaat gctcaacttc catcccggcg cacacctggg aaagatcagc
     3781 gaagaggatt gcatcaaaat catcgccgaa tccatcaata ttgtcctgtc gaaaaccagt
     3841 gacgtgacag cagtgatcga aaatacagcc ggccaaggtt cagcgatcgg tcaccggttc
     3901 gaacacctcc gggccatcat cgaccaggtc gacgataaaa gccgtgtagg agtttgcctc
     3961 gacacctgcc atgcctatgc cgccggctat gacctgaaaa acccggaagg atacgacaaa
     4021 gcgttcgatg agttcggtaa gatcatcggg ttcaactacc tgagaggatt ccacctgaac
     4081 gacgcccgca aaggactcgc ttcccgcgtc gaccggcacg aaagcctcgg caaaggggat
     4141 atcggcgaag acctgtttat ccggatcatg aacgatccgc gcttcgataa catgcccatc
     4201 atcctcgaaa cccccaacga agagatctgg cctgaagaga tccgctggct ctatgaaacg
     4261 gtgggaatct agaagggaaa agggaaaaag gaaaagactg cttatagctg actatagctc
     4321 aaactccagg taatcctgct gatctttcaa tcgtttattc attcccatcc cctgcaggtt
     4381 aacatcctgc gactttctaa catcgaattc cgcagtgagc cgctggccaa ccggtttgag
     4441 caattggggc cctgctgcac gggggctctt gatcgccggg tcgaccggat aggcattcat
     4501 caattccgcc gggtaagggc gcagcatacc ggtaacatcc gacagcggca gatcatcgct
     4561 caaccaggct ttctcaagcg acctgggcaa aatgaccggc gaacggtggt gcggtatctt
     4621 acggaggagt tcattggcta cggtcgtaat gatggcaaag gagcggatga tctcacccgt
     4681 atccggatta gcccaggtat cccagacgcc ggcaaatgca aatggtctct ccccttctct
     4741 caggtataac acgtaaggcc tcgagagctt ttcttttgtc gtcccttcaa tgaagcaatc
     4801 ggcggggata aggcatcgct gcgaacggat cggtttgcgg aaagagggtt tggtgatgat
     4861 cccccgggcg ccggtatact caggattgtc gtcaggatta tgatccccct ctgccctggc
     4921 gttgatcagc agaagcggtt tcttcgacca gaaaggtgtc agcccgaaac gaaaaagctc
     4981 cagttgcctg gggttttcat tcgtgatcac cggtgcatag cttcccggta cgatattgta
     5041 gttaggctga aagtcaaatc cttgcggcgc ttcgacgtta aaccgctttt cgattaattc
     5101 caccttttga accaaaacat accggccaca cataatccgc tttcgttaat ggtttaaatt
     5161 gtcgatttcc agcgaaatct cttccatctt cgccggtagc ggcgcatccg gcgacaatcc
     5221 cacaagcagg atcagatacc tgttctcctg tctcactact atcctgtttc ctttgttatc
     5281 cttacacgaa aatccctggt aggtattggt cagatcggta aatcgcttgt tttcagaaag
     5341 actctgccgc gccgactgca tattttcaaa cgattcagtg gaattggtat attcaagcag
     5401 aatgacatgg taatcctccc tggaaaatac catagcctcc ttaaaagcaa agggattgcg
     5461 gtaatcaatg taatagaaat tagacaagcc gatatttccg cgcagataag ctttgtttga
     5521 agatgaagcc tgcgctgagc aaagtcccag tatcccgggt atgtcgccgg aggttgaaac
     5581 ctttccgtca attatagcag ctaattgtcc cagtaatggc tttccgccat tctttttcga
     5641 tttccatgac aggatcacat aataccggct cttccaaaag gcgatgaaat catcagtaac
     5701 cgtcgattgt ttgccaaact ccccggtcca tgatgccttt aggtgattaa tgctgtaaac
     5761 accgtacgcg gcaccgtcat ccgtcatctc ataaatctcc gcacggataa cattctggcc
     5821 gggatcggaa taaagggccg ataccacgcg gctgaaccca tactcatgat aaatatcggc
     5881 cccgccgttg atcagcatat atagctcatc cccgttcatg accgtctgcg actgcaaagt
     5941 ccagccaggt aattctgccg gattgggcaa cagatctgtc agattctgac cctgaagagg
     6001 tatggcaaga aaataaagta gaataatgaa ataattccgg attgtgcgcc aaccacttct
     6061 caccataaaa accattctaa atctcagaga ctccatctca ataaaattct ttccggaaaa
     6121 ttcaaatcaa acttcaaatt cggcaacagc cagtttctcc aggttcatct ccccgagccc
     6181 atgttcatac gccatgtata cagtcaggat atcggccgga gcagcatcga ggcatgtcgc
     6241 gccgaaagca tcgacagcca ctgggtcggt tgaagcgact accaggtcca gctttttcat
     6301 cggccccggg ccggccggac cgttgcccgt gatgaattcg gtggcgtcgg ccacagtcag
     6361 atccggcttg cggaccaggt tgaggtcggc aatacactgg gccaggtact ccgggtcatt
     6421 cctcttgccc gatcccaggt ggaatgtcac attggtcttg cgcgtcgtca ggcccatcat
     6481 gtttttcagc gctccggtca ggatcgtcga agcatgatgc ttaaggattg gaatattgat
     6541 aaacacatca atatcagcga tcttccggat gatctcgaca tctttcagtt gtttcgcacc
     6601 tggaattatc ggcatcaagt taaaatcttc ctcattataa acagccggaa aaacattgct
     6661 ttcaacctgt tttaattcat tgatgataaa tctgtgcttg ctgaactgcg ggctcctctt
     6721 ccagtactct tctgcaaccg gctggagcag cgtcatttcc gatgccccgg cctcccagca
     6781 gagataaagt acggccatcg ggatatccgg gtgaacataa gcaccgggct catcgaacgc
     6841 agaattgatc agcagtccga ccctggcccc accagccggg atgaaccggc cgattccacc
     6901 gagctcttca attgctttgc gggtggcttt gtaatagtcg ccagacttca ctttgcagag
     6961 gtcgggcttc aatgagccgg aaaataacct gaaaggaacc atgttgagca tcaggcttcc
     7021 ggcagccagc cccgatccct tgtaaataaa ccgcctccgg tccatagatg tgattttact
     7081 gtacctggaa ataaacgttt gtctgccatt tgctggtatc gggttccatc atgggatcgg
     7141 taatgtattc ctcaatcgga cctcccgtct cagtcagctt gaaatcctta acatattcat
     7201 ccaaagcaat atacaggtac atcgtattct catatgggcc gaaatgggta cccataactg
     7261 cccgggtagc cggcagcacc ttgtattgga cacggccttt ggatttcacc tcgcggtcga
     7321 ccgggatgca gttctcaaaa acggtgaact ggctttcttc gtcccattta tggtatatcg
     7381 agagcggtgc acccgtctgt tgagcttttg aactttgttg aacagccatc agctcgccga
     7441 acaactggcc catttttgct tcaatatccg accagtagca ggaatcgacc acagaaataa
     7501 cggctttgga aggaaattca actatttgca aagcgggcgg gtcgggcatg gcttcgacta
     7561 cctctttcat tttatcgagg ccttgtttaa ataccggttt catcataccg ggcatcataa
     7621 cggccatata acgccccatc gggtaaggca ttttctgaat gtccacggcc cagtttacag
     7681 tggtcgcgcc gttctcttcc ttaagcagga aaatgttggt tgttcccggc ataccgaagt
     7741 cgagcgtcga tatcactttc tcatacggaa cactctcagt gatggtcata aatccattac
     7801 cgctcttttt gctggtccac cgcatcgtag cgccaacgcc tttctccgca ccttcataag
     7861 tattgaccat ttggggatcg gctgcctgga atggcgacca ggcctcccag ttccggaaat
     7921 cgttcacctg cttgtaaacc aggctggccg gtttgttgat cacaatcgat tcttccatgt
     7981 aagatttgga aggaaggaac agggctacca cgagaaacag cgcgatgagt atgagcaaaa
     8041 taattccgat gatttttaat gctttcataa tggttttaat tatagatgat tacaatatcg
     8101 agttaacctt gtcaaaatta aaaaataact ggtatcctga tatactgtca atgtgtcaag
     8161 gaattaaaat ttggtctatg caactgtttc aatgatatat atgtcttata attattacta
     8221 taaaccaaaa gttcaaatgc catgtcaaag gtcttattta cactttactt tagcttgctg
     8281 attgccattg caggctcagg tcaggaaacc ggggtcctga caggcgtttt aacggatcaa
     8341 tacaccggtg atcctttgtt tggagtgaaa gtgcgggtct gggagcaggg aaatgcagaa
     8401 gcgcaaaaga gttcggtttt tgtggctttg tcggatgagg acggcatttt cagcctggaa
     8461 ttgccggccg gtcagtacca ggcatcgttc agccggtatg ggcttgataa acttgagatt
     8521 cctggtatca tggtgttagc gaatgatacg gccatactta acacaacctt gctcccggtt
     8581 gaggtggcag tagctggcgt ccttgttaat actgaacctg tttatttaga aatccaatgg
     8641 acgccctcct ggccgtcaat aatagaaaaa tcaatagatt atggccctgt aaatgactac
     8701 ttcgttttca tgaatgcagg tagtcaggtg gcagttaaat tcgacaataa tttcgatcat
     8761 gtaatcggag ggcgaatttt tgtgggtgat ggctcactcc ccggcccatt cctggggtca
     8821 gattttctgc tgagaatata tgatgatgat ggaatggggg gacttccggg caacaccttg
     8881 ttcgaagaca ctgtgacggt tgaccagtat ggctgggttg gattggatag cctggatgcc
     8941 tttacttcgt cggatcatta ttacctgtcg atgtaccagt tgaacgaagc gccggaatgc
     9001 gcgccggttg tcagcactca gtatcctgct aacagcagca gcttgatgaa accgggaagc
     9061 tacaactgga tgccttttcc cccgggaaat gccatgatcc gggcatgggt ttcagctccg
     9121 catgacagca ttcacgtatc aagttacaga gtaggacgct tttctaattt cgatccaaat
     9181 ggtgatccgc aatatggaac tctgactgaa ctggcctcaa ccaataattg tttttataat
     9241 gataatgcct ttccgggtct tccgcaaggc tggtatgcct atggaattaa agcattgtat
     9301 aacaatgggc aatattcgcc atatagaatt tcgggtatcg tttataatcc agatccatgc
     9361 aacccttctt ttactataga acaatctgat ggttcagtaa gtggcacttg tcatatctca
     9421 atgaccggca tatcccttcc ttttaagaat tattcttttt tagtgcagac tcccggtaca
     9481 atcaatttat caccgataaa aatgggaagt tatagtattt cattatataa accgggttat
     9541 gagaaatgga tgatagattc cgtggggtta ttttccgaca cctcaatcaa tataacatta
     9601 caggaaacga tctacccaat tgaagacctg aaagtcaatg aatacacagg catgctcgat
     9661 tgggagaacg attttgtcag tcaatatgaa tggcaatgtc cagccgattc aatttgccac
     9721 gagttgatca ccccggagct tgacctactc ggtcttaatc aatggatcat gacggtaaca
     9781 tacatgactc ctcttgacaa cgatccccca attctggaat attcgaccga ccatggccgg
     9841 acctgggctc tcctgcatga gtttcctctg caggaaaact ggactactgt tgacattgac
     9901 ctcgttgttt tcccgaatga atccgaagga tcaggaatca tgttccggtt gcatgatgtt
     9961 tatggcgctc catattacac ggatatcagc cggataaaag tttggctgcc aagtgtcagg
    10021 tgtcatcctg aaaagtatta tatcaccctg gatgaaatgc cggaagggca atccgattca
    10081 acattttatc agtctgacaa cctggaaaac gggcaaagtt accgtgcagg ggtatttgct
    10141 gaatactcca ccggtaccac cgatacggcg tttgtggaat tcacctacca tgaacttttt
    10201 ccaccggaag acctggcctg ggaagaagaa aatgataccc tggaattctc ctggagcgta
    10261 ccatccgggt cctggaataa cgacaaggca ccgggcgatt atccaggtgc gcttagcggc
    10321 tatgctctga cttaccagac accggataac ttgtaccggt ttgagatcga cggccctgcg
    10381 acgacttcct tccgctttga aaaaccggac tgcgattctg ccacagcaat tcttacggct
    10441 gtatatgatt tatcagacta tggttatccc ggaagcctgt ttgaatcgaa acctgccggc
    10501 ccgctgcaaa taaaattcga cggcccggtt gaaaatgaat tctttgagga ctggtcggcc
    10561 atcagcctta tccataactg ctggaaaatg accggaaacg gcctgtcgat tgaagccggg
    10621 cagggctttc cgggagctgc acttatctat cagaacccac cttcccacta ccaggttttt
    10681 ctgaccagcg tgccgataaa tattcctgaa agcgaagatg cgcaacgttt cctcgaatgc
    10741 gatctcaccc tctttgccgg aggctacagc ggctatgaga tcatggaaat tcatctgcaa
    10801 caggccgggt cctcctattg ggatgtggtg caggaattct ccaattcatc gggaagttac
    10861 gactggagcc attattcatt ggacttatca ggatatatca gctcggatga attcagggtc
    10921 cggtttaaat ttgaagggat tggagccgaa ccggtacagt ggaaagtgga caatattcat
    10981 ttacacagag tttgttccgg accaacaact cttcaggctg aattgaccgg tgagaatgaa
    11041 gtcagcctga actggctccc gaatacagcg accaaatcag ccacatcatt tgagcattac
    11101 aacatattcc gaaaaacggg ccaggatggc ttttcgctcc tggcaaccac taccgatact
    11161 ttctaccttg attatctcaa ggaaggcgga aggtacagct accgggtcaa tgcggcgtat
    11221 aatgataatg gtatcctttg cacctcctca ccttccgact cagcctggat cgtctcaact
    11281 tatgatatcc cggagaatga catacatggc cgcatctctg tttaccctaa tccggttaac
    11341 gagaggttat ttattgtatc agatgaaatg atctatggaa taaatcttta caattcattg
    11401 ggcatctgcg tcctggatat ggataaatcc ggtttgaact ttgaaatcga catgaaatct
    11461 ctggccaatg gaatttactt tctccggatt gaactggaaa acaaagatta caacgcaaag
    11521 atcattcatt aacttttgac agattcatcc gccggtcggc ggaaaaaaaa aggctgcccg
    11581 gtagatcggg acagcctttt ttattcgtat caaaccaacc tctacttcac cttatccacg
    11641 atagccttga aggcttcggg gttgtgcatg gccaggtcgg ccagcacctt gcggttgatc
    11701 tcgatattgt gttccttcaa tttgcccatg aaaaccgagt acgacatatc gtgctccctg
    11761 acggcggcgt tgatccgctg gatccacagg gcgcgatagt cgcgtttttt ctgcttgcgg
    11821 cccacgtaag cgtaagccag gcttttctca taggcgttct tggcaacggt ccagacattc
    11881 ttccggctgc cccactggcc ttttacccgt tccagaactt tcttctttct tgctttggat
    11941 gctactgcat tgactgatct aggcataacc tttcattttt ttacttcagc ggcccatgtt
    12001 tcatgactct ccgtgctgaa agtaagttaa cactcattga attactgaag catgtctttt
    12061 acgttcttca agtcagcgcc cgatacttcc gtgaagtgtc ccaggttgcg tttccgcttg
    12121 ttcgacttct tggtcaggat atggctcttg tacgcatgct tccttctgat tttaccggtg
    12181 ccggtctcag caaacctctt ctttgccccg gattttgact tcatttttgg catctttgtg
    12241 tatattgatt gttcgattgt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnattgttct attgttaaat tgtccaattg ctctgatccc tactttttcg gtgcgatcac
    12421 catcatcatt cgcttccctt ccagcttcgg cagttgttcc acctttccaa attcttccag
    12481 gtcggcggca aatttcagca gcacatattc cccctgctct ttgtagataa tgcttctgcc
    12541 tttgaagaaa acatccacct tcactttagc cccttcttcc aggaatttta tagcatgttt
    12601 cagcttgaaa ttgaaatcgt ggtcgtcgat gttcgggctg agccggatct ccttgatcac
    12661 aatcttggct gaatttgcct ttatctcctt ttgtttcttc ttcttttcat aaagaaactt
    12721 cttataatca accactttgc agaccggtgg atttgccgtc ggggatattt ccaccaggtc
    12781 gagctccaac tcttctgcta ttctcaatgc ttctcggatg ttatagattc cggtctggat
    12841 attttctcca accaccctga cgacgggagc tttgattaat tcattcacct tgtaaggttc
    12901 ttcctttgcc ctgttgtccc ggaagggtgg cctgcctttg aatggtaatg acgttgctat
    12961 aaaaaatcct cctgaaatta gttaaacatt ataaagccga ttcctcatca aaggtgcttc
    13021 aatgattctt ccacttcgtt ttttaccaga tttataaatt cctcaaccgg caaagctccc
    13081 atatcaccct gtccgtgcct gcgcacagag acagtgccgc cttcttcctc tttttcccct
    13141 acgataagca tatatggaat tttcctgatc tcggcatccc ttatctttct gcccgctttc
    13201 tcactccgct catcaataag ggcgcgaata tcggaatttt gcagcaaagt tataactttt
    13261 tttccatatt cggtaaattt ctcgctgata ggcagtacga taacctgttc gggtgtcagc
    13321 cacagcggga aattcccggc acaatgctcc agcaataccg cgacaaaccg ctccatcgag
    13381 ccgaatgggg cccggtggat catgatcggg cggtgctttt cgttgtcgct gccggtgtat
    13441 tccatctcga accgttcggg caggttgtag tcgacctgga tcgtccccaa ctgccatttt
    13501 ctccctaacg catcgcgaac cataaagtcc atcttagggc cgtagaaagc ggcttcacct
    13561 tcgacgatct cgtaattcag ccctgcctcc tttgccactt cgatgatggc attctgcgct
    13621 ttttcccagt tctcatccga tccgatgtac ttctccgtat tggcggggtc acgcagcgaa
    13681 atctgggtga tatagtcctt gaagtcgagc gctttgaaga tcagcagcac aatgtcgatc
    13741 acgcccttga actcgtcttt gagctgttcg ggggtgcaga aaatgtgggc atcatcctgc
    13801 gtaaaaccgc gcacccttgt caggccgtgc aattcgccac tctgttcgta gcggtacacc
    13861 gtgccgaatt cggcaacgcg caagggaagg tctttatacg acttcggttt gcttttgtag
    13921 atctcgcagt ggtgcgggca gttcatcggt ttgaggaaaa actgctcccc ttcgatgggc
    13981 gtattgatcg gctggaaaga gtctttgccg tacttggcca ggtggccgga ggtccggtag
    14041 agctcaacat tcccgatgtg cggcgtaatg acctgcacat agccggcgcg gcgctgcacc
    14101 cgtttgagga actgctccag gcgttccctg agctcggcgc ctttgggaag ccataacggc
    14161 agccccgctc ccactttctg cgaaaaagtg aacagctcca ggtcgcggcc caggttcctg
    14221 tgatcgcgct ttttagcctc ctccagcagg agcaggtatt cgtcgagttc tttctgcttt
    14281 ggaaatgtga tgccgtagat gcgggtgagc atctgccgtt tttcgtcgcc acgccagtaa
    14341 gcgccggcga tattgagcag tttcacggct ttgatgacgc cggtatcggg cagatgaggc
    14401 ccgcggcaaa ggtcagtaaa ggtccctgat tcatagaatg tgatctcgcc gtcgttcagc
    14461 ccttcaatca gttccagctt atactggtcg cctttgcggg tgaaatactc caccgcttcc
    14521 gattttttca cttcacgacg tttgaacgac tgtttttcgc gggccagggc cagcatacgc
    14581 tcttcgatct ttttcagatc ttcttccgtt atcttataat caaggaaatc aatatcataa
    14641 tagaatccgt tctcaatatc cggaccgatc ccgaatttga cgccgggata aagttgttcg
    14701 atcgcttcgg ccatgagatg agcggaggaa tgccatacgg tggctttgcc ttcggtgtca
    14761 ttccaggtaa gtaactggag ggaagcgtca ttttcaatgg gccgggtagc atcccagacc
    14821 tcgccgttca ctttggctgc cagcacattt ctagccaggc cctcggagat gctcatggca
    14881 atttccatcc cggtaacccc ttccgcgaat tgcctgaccg aatgatcggg aaatgtgatg
    14941 tttatcatgt tccttttcgt ttttgcaaaa attgggtgca aaggtaagaa aaacaatgga
    15001 accggaaaaa tattcctgca ttaaagtatt cttcctggaa cgggcagttt aaacacacat
    15061 attgcgccgg atcggcgggt gcggatcgta atcctcgatt acgaaatcat cgacggtcag
    15121 ctgatcgaaa ggcttttttg cgatgcgtag ccggggaagc ggccggggtt cccgcgacat
    15181 ctgctcgaga atggccgtga cgtgatcata tttctcttca ttgccgtcgc tgggggcatt
    15241 tttatcgatc catcgtatca catcgaggta atcttcgcgt tgggaaacat tcctgatctt
    15301 ttgctgaacc tctttaaaat tggctttata ccactgggtc ctcttcccga tgctggtata
    15361 aaaatgggca tcgccaaagg tgtggataaa ggttttgggt accatatccg cttcctgtgc
    15421 aatgatctgg gtaagcgcag catagctggc aatattaaaa ggtacgccca ggaactggtc
    15481 gcagcttcgc tggtataact gcaattccag ctctccctct tcattggccg tcgcctggta
    15541 aaggacatgg catggcggca gcgacatccg gggggtgtct ccgggattcc aggaatcgac
    15601 aatatgcttt ttccccgtcg gtttcttctt caggccggca atcattttcc cgagctgatc
    15661 gatttcaatg aaagcgccct tttcatggtc gaagtattcc catcttctcc attgtttgcc
    15721 atagatcggt ccggcatctc cccacctggc cgcgaattct tcatcttccc ggatcctctg
    15781 gccatattct tccatcgcct tatcccaatc cggtgaatac ttcaccaggc cttccggaaa
    15841 gatacccgct ttctgcatcc cgggaaggtt atcctggaaa gaatcgggtc gccagatcgg
    15901 gcagttattg tcttccaggt acttaatatt cgtgtcaccc cgcaaaaacc agatggtttc
    15961 atgaaacatg gaacgggtag ccattttttt tgtggtcagc aaaggatatc cttccctgag
    16021 atcgtattcg ttttgatacc cgaataaaga gatgatcccc gatcctttgc ttccgccttt
    16081 aaaattggag cggggagagg tcaggattgc tttggaatga tccagatatt gctgtgccat
    16141 tttttgtcag gttagttttt tgcaggttta caacggtcaa aagtagctct aaataaaaat
    16201 catcatccaa ttcttatatt cacagtaaag atgaaattat taacaaagag gccgttttaa
    16261 ggccggatga atacggctca ttacgttgaa tattaggttc tagcccatca aatcaagcag
    16321 ctttgagaag agttttactg tggcggtggc gtctcccaat gcggtgtgcc gaccttcgac
    16381 ctcaatgtca aaaagagcaa aagcatcatc ggaagatatt gccttcttct caatacgacc
    16441 cgccaggtaa agataatgca ggatggagga ggtatcgatg agccggtggg aaaatatttc
    16501 gctgaagtca atatggttag cggaaaagaa ctttttaaga aagccggcgt cgaagtggac
    16561 attatggccg gccagggtaa ttttctcctt gccgggaaag tgtttacccg taaacgacat
    16621 cacctggtcc atggcttcag gcaatgaaag cgctgtttga aggtgtttcc cgatatcgat
    16681 tttattgatg gataaagctt cctgtgtcac acaattaata ccatcgttaa ctgcaatttc
    16741 cagtgaatca atgatattcc tatcttccca taccaccaaa gccaacgtca gcagcgaatg
    16801 tttatcggga tccaggcccc cggtctcggt atcaatgaat agcagtctgt cgggcatttt
    16861 aataattatt ttctggtgaa gaatcacaaa gatacccgga tccaattatg ctttttcaat
    16921 gtttttccgg aattcattca caatttcagt cgctttaaca agatttgact gaaggaccat
    16981 tactttaaca ccaccggcaa aagtgggttc gaaaacaaat gtgttgaggg tggaattctt
    17041 caggaaacaa tctatttcat tgtcattcag catagtctgg agcaattccg cttcccatag
    17101 agtacctgag aaaatttctg taactggatc gtctggcata ttaatattgt ttatttaatg
    17161 cgatgttgta gttgtggtta cgttcatata cactatacga ttcctgtccg aatccagtgt
    17221 gcccaatcgt ttcgaccatt cctttccgcc aaccgggctg cggattcaaa gtcatactca
    17281 agggcaactc tttcaacaga tactgattcc ccgttaacgt tcaatatcga gtaattcgcc
    17341 cgtggactgt aattctgcat tcgatgataa acaggcaaat cgtcatcata agccggcaga
    17401 ccgacactgc cgggattgat gatcagttta caatctgttt gaacaatgtt cggtacatgg
    17461 ctgtgcccgc atacgattat tttttgtgtt ttctctttca gcaattcgtc aagttccatc
    17521 tgtgacctga tatcaatatg attttcagaa actttctcga gcaggtattc cgtatcactc
    17581 agcggagagg catggcaacc ataaaccagg tcgttgaaga tcatatcgaa aggcaacgac
    17641 cgaagccagt ctgtcatccg attgtctatc tgagatttga catactccag tgttttatta
    17701 cccgaatctt tcgttaaatt ttccaaaata atccggtctt cgttcccgga gatattaatc
    17761 gcgtgacaag cattcagcat ttcccatgtt cctgaaggat taagggggcc atacagagaa
    17821 tcgcccaggt ttatgattgc atcggcattc ctggccctga tatcctccag cactttgctt
    17881 aaagcataag agtttccatg gatatcggat agaatggcga ttttcatgac caatttcctt
    17941 tgcgctagat gaacagcaac aaactgaccg ccatgactgc cattccggcg accaggccgc
    18001 cgatggcaat gtggtgtttg ccgtactcct cggcggttgg caataattca tcgagcgaaa
    18061 tatagaccat aatgcctgct acgcctgcga aaatcacccc gaaagtcgaa tcattgaaaa
    18121 cactgcggag gataaaatag ccgataaacg ctccgacagg ttcggcaagt cctgataaaa
    18181 aagacaacca gaatgctttt ttacgatttt tcgtggcata ataaaccggt accgaaaccg
    18241 caatcccttc gggaatattg tggatggcaa tggcgacggc aatactgatc cccaatgtcg
    18301 gatcggtcag agcgcccata aaagtcgcaa ggccttccgg aaagttgtgg atggcgatgg
    18361 ccagcgcgga aaacattccc attctcagga gcttccggct tttgtccggg tccaggttct
    18421 tctcgtcgat attctttatt tcatgggggt tttcataaga gggaatgagt ttgtcgatca
    18481 gcgcgatcaa tgcgataccg gcgaaaaaag ccacaacggt atagatgttg ccggctttaa
    18541 gcccgtgtgc ggcgaccaat gaaactttgg ctttggcaaa gatctccacc agtgaaacgt
    18601 agatcataac gccggccgaa aagcccaggg aaccggccag aaatcccgga ttgaattttt
    18661 tcgacagaaa cgacatcagg ctgccgatcc ctgtggccag gcccgcaaaa aaagtcagcc
    18721 caaatgcaaa taacaggttt tctgtggtca ctttatatga tttaaaacag ggcaatcaat
    18781 cgggatagcc gattctaatg gcaaaagtac aataaataat atgcttccac attgtcgaga
    18841 taggcgccat caaaacccgc atccaatatt ttcttcagat atgaatgatc gttaccgaaa
    18901 ataatatcct gccattcctt ttcccagaac tcaacccaga tttcgtcgtc gtaaccctca
    18961 tattttttct tcagccagtg cgggtggtga agctcccaat ccggcttcca gtagtaacgg
    19021 aaattttctg cggagccgat atttacataa gaaataacga gccgttgtgc gccatttgct
    19081 tttcttttca ggagattgat ctcagaagag gtaaattccg tttcgttgaa aaacagatca
    19141 atcaatacaa tatcaaagtc agttgccgag atttcatcga tcatcacctg ttttgaagca
    19201 aagctgtcgg tactgataag atacagataa ttttgcgcat caggcaggtt agtgatatcg
    19261 aatgcattcg gatgaggaac actgtcgggg atcagatggt aatgataatt actgcttata
    19321 cgcggaaagc agataaatcc ttcggtataa ttccggctca gcgcatcagg gatattatca
    19381 ctgtcagaaa tatattctga caccaatatt tttttgtaat ccttgagccg ttgaagcatg
    19441 gctatgcgtt cattatcggg cgtgtaaggg ccattataga ataattcttc gactccgaag
    19501 ccgtcgatgg cagacaggta gcttgtatta atgccgtcgc caggttccaa attattaaaa
    19561 gcaagctcga tcccattttg gggaatgatg ataaaaccgg ggtcataatt tcttgcatag
    19621 gcggagatat ccgaaacaaa ggcctgcatt ttttcggcgg ccaggtccga tttcttttcc
    19681 ttagaacaac ccagcagtgt ggctgtcaga atagaaatta tgatgattct tttcattgcg
    19741 ggtatttata ttgctccggc gtgggtggtg ctaagagtgg catacccctc ataaatttat
    19801 ataatcctga gccgttaaaa cagtaatctg agctttctta tagtccttta aattccgggt
    19861 cagaaaaacc tcaatttcat tctgcaatgc ggaataatac tgaatagcat cttcaaaatc
    19921 agcgaattca gagttcaacg ccaggtcaat gattttatca ttcaggggag taacatgaac
    19981 caatacttta aactttctga gaatttcctt tgcctggttc tccgttctct gctttgaaat
    20041 caaataatga agatttgcta aagacaaggc agaaacataa atttggatct ttccttcgtc
    20101 tgcccatgta aataattggg ctgaagccag ataaaacgga tctcttttcc caagcagatc
    20161 ataaatgatg tctgtatcga caaaaactct ccttttcata aatacttctc ctgaaggtgt
    20221 ttatgataat tgcctttggg atcatcattt tcggaaatag taataacacc actaatagac
    20281 aggaccaggg gtgagatttt ttcaacgggt tttgattcag aggtaatagc cgataaatag
    20341 ttctcaatca attttgataa actggtatcg tgatcatcag catatttttt tgcctggtca
    20401 attactttct ttttgagccg taatgtcaat ttggtttcca tgatattatt ttaagacgta
    20461 caaagatata ataataatac gtcaagatca aaaaaatgtg aattgggttt acaccgggga
    20521 aaagtaaagt tagcatcttt cccctcccct tgatcccctc ccatgctgat attgccttga
    20581 aaaaccggat tatcgcggga ggggaaaatt gtgcgggtgc catcgcctt
//
LOCUS       DAOPNH010000017        20555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2451_length_20555_cov_6.119171, whole genome shotgun sequence.
ACCESSION   DAOPNH010000017 DAOPNH010000000
VERSION     DAOPNH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_20555_cov_6.119171"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="PKZ74_01935"
     CDS             <1..1827
                     /locus_tag="PKZ74_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM91538.1"
                     /translation="RKTERAERAFRNINNNDRINSVIKDFAQNPEMISSYPEKLYRDG
                     EYQYLIITPEQFAPSFQALTDLYRVRGYITEIATTESIDATMPGQDLQEKIRNYIIAA
                     VQANGVEHVLLGGDVEHIPHRGFYCHVQSSSVYEDWNIPADLYYSALDGNWDTDGDGI
                     WGEIGEDDLLPDVAVTRMPISTSTQLQRMLNKTIMYQDQPVAGEMRDIISAGENLWSD
                     PLTYGEDYLELLFGWHDDNGYVTDGIPEDYDVNRLYDSQMYWDKYTLMNAINQGSSFI
                     HHSGHANQTYVMRFDMSDISNENFSQVNGTDHNYALIYTHGCLCGAFDESDCIGERMV
                     LIDNLAVAGGFNSRYGWFNEGQTEGPSAHLHREFVDALYHEKEWRIGAAHMISKIETS
                     VWVNAPGQWEQGALRWCFYDCNIFGDALLGVWTEEPVSIETTYPEEIYPGETTVQVSI
                     ANGDGPAVGFNCVIMSAGEMIGCATTDESGQAEISVPEGFIDHLDAQLIVSGYNCLPH
                     TYDLNIVVGIDEPYVIDYLNIIPNPFRDQTSLVYQLEEAGSIEVTFVNLAGQMIEKMQ
                     FDGKKGINRFNINTTEWPEGMIISKITGENRTDYYPLLHIKN"
     gene            1936..6498
                     /locus_tag="PKZ74_01940"
     CDS             1936..6498
                     /locus_tag="PKZ74_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM91539.1"
                     /translation="MNRILLLSSFILLMMSGLDAQTYRYDDSFGPQGFSLVSHDRLGV
                     TVNFSIREFSMEEMDIKGDRMQHISLPGHFLPGDEGTPDLPGDGRFIAVPNGAKPVIS
                     VKTARREVIRNVDIAPAPNLPLDNDDRPVVYKKNAKIYSTDAYFPAEPVKLSGKMSIR
                     GVDAFVLGITPFQYNPVKRELVILRDMEVEIRFEGGSGDFGEERLRSRWWDPLMQDMF
                     LNSADLPVVEYSKRVTSPNRATGCEYLIICPDGEEFIAWADTIRRFRNQQGILTEVFT
                     ISQVGGNNAMIIEDFINNAYYSWEIPPAACLLLGDYGSLPQNAITSSLLNDHPDDYNP
                     YISDNPYADVTNDLLPDIAFARITARNAAELELMIGKALDYERNPPTSEYFYHHPITA
                     LGWQTERWFQICSEAVGGYFRTKGKEPIRINEVYGGDPDADPWSIAGNTPAILSTFGP
                     EGLGYIPATPGELGNWEGGNANDINQAVNNGAFLLQHRDHGNENGWGEPYYTRNEISG
                     LQNTELTFVMSINCQTGKFNSGSESFGEKFHRYQFNGEASGALGLIAPTEVSYSFVND
                     VFAWGFYDNLFPDFFPQFGTTPPSRGMYPAFGNAAGKYFLFASGWPSNPQHKSITYKL
                     FHHHGDAFTGLFSEVPQALTVAHDSVMLAGTFFFDVEADEGSLIALSVNGELIGVATA
                     TGEPVPVEIIPQNPPYILDVVVTKENHFRHHSTVQIIPPQGPFVISQACHVNDTQGNG
                     NGLLDYGETAVFDMTFDNLGNESAEDVVVHIYTDDEYVTIIDNETFAGTIAAGEVKEV
                     EDAFSVTLADNVPDGHVVHFEMEATDGDSIWISTFAVKTHSPVLVYAEYAVADTGGNG
                     NGRLDPGEEVGIVISLKNNGTASAYDVYGLLSSTDPYIEILSDSVMFGEVGPGETVTG
                     AFTATAYVITMPGHEAGFDIDFSGNLGIAAADDFSLFIGLFPVLILDLDQNHNSADKM
                     KAALDDLRIFSEILDEMPEDLSQYKTIFVCLGTYSDNHILTADEAGKLIGFLNGGGNL
                     YMEGADTWYYDQLYSPTELHPMFNITGEDDGSNNLVTVNGITGTFTEGLSFFFSGDNN
                     YIDHIVPGEGAYSIFTNSNPVYNLAIVNDAGNYKTIGTSFEFGGLMNNLNSSRKDLML
                     KYLDFFGMDAITQGPLLPVGDTLACSGSSSGIYTTHPVPDAHYYIWELLPASSGTITG
                     WDTTVTVEWTPGFEGTATLRVCGMNENGLGPLSPGLAIHHFTSPAATVAFSDPVICPG
                     DTTFMNITLTGSQPWQMLISLGGYQINLNPNKPIMDGIPLSPTADLEVTILALSDATG
                     CIATGFPSNTLQVTQIPGIPAIPAGPVYVDLYGATQSEYAVADTMPANNYEWILLPQE
                     AGNLTLSDDGTVCTVNWTAGFTGEATLKMRGVNECGEGSFSDPLTVNVINSSGVKDGN
                     VGPAISVFPNPSSGSFIIQINSNADISGILTLTNSSGRKILETEKINFSYNARIPVDA
                     QFLSPGIYFIEIKTDKTVYRDKVVVK"
     gene            6642..10736
                     /locus_tag="PKZ74_01945"
     CDS             6642..10736
                     /locus_tag="PKZ74_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM91540.1"
                     /translation="MKANLNIIRIAAWFIIAGLFLINPVFSQKITYSDPLNKAGFSLG
                     QETRGGVTVNYTIETFTFSPVDINGKGMKNVELPGNFLPGDAGAPNLPGSGRYIAFPK
                     GAVPVLTIVSQRTESFKNVDIAPSPEIPLDTDREPLKYPVRTDIYTTNAYYPATPVQL
                     SNPTQIRGVDVVMLGVTPFQYNPVTKELIVYRDLEIRVDFQGGSGQIGEERLRSRFWD
                     PILEDAVLNHKSIPAVDYSRRVLNPNRSNNGGDYLIIVANSDDYMNWAQEIRSFRIKQ
                     GIVTEIRTLEDVGGNSPAIIENFIDDVYENWDPVPAAILLMGDYGSNAEVNITSPIWD
                     SYCVSDNIYGDVDEDDMPDIVMARMVGNNNTEMETFATKFINYETNPPTNPSFYDHPI
                     TALGWQTERWFQICSETVGGYFKNVQGKNPVRINEIYLGNPGSDPWSTATNTQVILDE
                     FGPNGLGYIPATPQELGNWSGGNATAINNAINSGAFLLQHRDHGEETGWGEPQYGNGN
                     IGGLTNTDLTFIFSINCLTGKYNWSGECFSEKFHRYRYNNVNSGALGVICASEVSYSF
                     VNDTYVWGLFDNMWPDFMPEYGSTPAERGLLPAFGNAAGKYFLKASSWPYNSENKEVT
                     YNLFHHFGDAFQTLYSEVPQEMTILHDGIILAGLNEFTITADAGSTICLTVDDDIIGL
                     ATGTGNPQVITITPLSVGTTVTLTVTKTNYFRHEEDIEVIPAEGAYCLYGNHTVADGI
                     GGNGNGLVDYNETVGLSLGIRNVGMSDGDGVTVTISTLDPFIAIEDNTEFYGVIPSGQ
                     TVVVDQGFSFYAAENTPDQHQVLFSIVSTDGGNVWNSQFMLKVNAPVLNINAVTINDS
                     ENGNGNGILDPGEQAEMTVNYTNTGHATAYDVDVFMEGQSGYIEISNPEQNFASIGFL
                     GVFNKTFDVSVDEITPIGININFVNDLSMGNFYSSKTFIQKVHPLIEDFETGNFNEYD
                     WQFTGTLPWTTSNQYPYEGNFSALSGAISHGQTSEMKISYEVMSADSISFIRKVSSEA
                     TDKLQFYIGNTLAGEWSGTNEGWRREAFAVTPGYKTFRWVYTKNASGSGGADKAWLDN
                     IHLPSPVALTIWAGPDEDICTGEEFQISGSYGTDYNTIEWTTSGTGTFTDNTQMQPVY
                     SPSSEDFNNGSVVLTLTLTGDGDLIVSDEMVLTFNAGPEAPQAIEGPDYVDLFITTTS
                     EYLTVGIEGITVYTWHLEPADAGTIEGSGLQSTVTWDPVFAGTAYISVSGNDECGEGD
                     VSEAFEVTVGNSVGYSEDENSFGLSVYPNPGNGSFQVMIATPEQNAVTLKVINILGEV
                     VYKSNQKVNGAAIIPVNIENLANGVYLLSLEGDGVSLNRKIVKR"
     gene            complement(11110..15021)
                     /locus_tag="PKZ74_01950"
     CDS             complement(11110..15021)
                     /locus_tag="PKZ74_01950"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPM91541.1"
                     /translation="MKSQLTLTVILLSLLTGVSSYAQVPDRFKKLNTAIDNQGYWRKA
                     AELGLTRLNPMNSAPPAVYTGSGIRAVSVITEDSPDVVIITGNTAQSENSVFVNPQDA
                     NNPLNSNNSSTQPGGGMTFYGSDYLYSFDEGLTWQGSVQGAGGTNQGDPTTAIGLNGR
                     YYIGYINNGMGQGVSYSDNQGATWTPVTVSSGGGNTLDKNHMWIDNSPTSPYEGTLYD
                     AWTPFGGPNDSEIELSRSTNDGQSWSTPVKISAGANAGYLCQGVNINTGPNGEVYAVF
                     CIYDNWPSDEDAIGMARSFDGGQTWESFRIITNIRGIRSTGTGKNMRVNAFPVMTCDI
                     SSGPNRGNIYVTWTNVGVPGVNTGNDVDVYMIRSEDNGDTWSEPIRVNQDPQGAGKKH
                     YFGWITCDPATGTLSMIFYDDRNVSSSQVEVFCANSIDAGDTWEDFKVSDVAFSPSPL
                     PGYASQYFGDYLGISARNGKVYPVWTDNRSGTALAYTSPYVTSTMMPPTALNAQLNDE
                     TGQVNLTWTHEAGPTFDHYNIYRGFSLIGTTVLPIYSDMLPGYGSYRYQVTAVYIIEG
                     ESAPAIADVQWGDAQAETDPASIEVFVLPESTSSFIMDLANTGELPLEYTSTFSLPGN
                     AREAGLAYCTGIGGCGGEGIAGVVYGDVSNFSECNSYSDYSDLSHLVSLGDSFEITVQ
                     NITNMYSEDVCGIWVDWNGNESFLDDGAITVSGSPGPGPYTATITVPDNAANGEVRMR
                     IRIRRGGTLSPCGFAPNGEVEDYSLNVLGWVTANPREGEIDAGETQPVTFDFNSAGLA
                     LGTYLAHFTIISNDPDNGELVVPVTMHVDNAAVTITSDKDSLCLGSSTTLHANITGGS
                     GNFTYSWTSDPAGFVSDDPDPVIEPTVTTTYFVEATDGNIILSDQITITVLAIPDLNL
                     GNDLFACVNDEVVLDAGAGFQQYFWSTGETAQSITVTGPGSYWVTVLNENECAATDTV
                     NVIFNPLPEISLGPDQQFCQGTTVMLSAGTGFQSYLWNTGASSYYINAGEPGEYWVEV
                     TDNNGCRNRDTITLAVLPRPEVDLGEDRIFCEGTTVLLTPGSGYEGYLWSNGATSASI
                     DTGLPGDYWVEITDETGCKNTDTVTLIMDPLPLAPQITSGPQSVDLYVILVSDYTLAE
                     SAYADSYEWNISPAAAGSVDGNGTTAQVTWNTGFTGTADITAKGVNDCGNGNDSQVYS
                     VSVYSSQGIGEPGAISSVKLFPNPNEGNFVLEMNSKEEQELIFSVASAGGNKVLETGE
                     KLGKGVIKKSFNLSAQPAGTYYITISDKENKILGRLQVVIK"
     gene            complement(15133..18639)
                     /locus_tag="PKZ74_01955"
     CDS             complement(15133..18639)
                     /locus_tag="PKZ74_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPM91542.1"
                     /translation="MKKIYILMLTALILTSGAMFGQTLVLKPLDDPRLGKIVNTRVDC
                     NRYYKEMAKLGLTTLNPVRSVEPAVYTGSEIRALSTITEDSPDVPVTSVNSTQSENSI
                     FVNPTNNQMVLNSNNSTQNPVGNLYGANYFLSEDGALTWGGSVNGAGGSNSGDPATAI
                     SLTGRQYIGFIDNNYGQSVSYSDNGVNWTRITAATQNGDLLDKNHLWIDNSPTSPHEG
                     NLYVAYTDFGASGWPIEVARSTNEGLAYQTPINVSSAVNAGSHCQGVNIQTGPNGEVY
                     VLWAIYDSWPSDESSMGLARSFDGGASYEPSYRIINNIRGIRTTSIVKNMRKNSFPSM
                     ACDISNGENRGNLYIVWANIGVPGINSGSDVDVYFIRSTDQGLTWSTPVKVNQDASGL
                     GKKHYFPWITCDPENGALSVVFYDDRNVGSTQCEVYCANSFDGGETWEDFKVSDVAFT
                     PSPIPGLADGYMGDYLGISARGGWVYPVWADNRTGIVMSYCSPYQTNPINKPTNLTAS
                     VTFETGITDLQWAYEEMEGFSYFKIYRNNDSIAMATDTIFNDQLPAYGDYVYRITAKY
                     ADGNESSGTNSVNVRWGDAQISAGPMEIEEFLMIDNTVTNYVTVNNIGQLPMDYTIGM
                     FIPTKAGQDPKAYCTATAQCDEYIARVTLNDIDNQSECVEGLGYSNFTNISTMMSVGS
                     SYEITIYNGNLSYPDDQCGLWVDWNQNEEFEDNESIPVNGSPGVGPYTATITPPVGAL
                     AGETRLRARIVYNMTPVPCGALSWGETEDYSVFLQSWLAVNPLEGTIPAGESAEIAVT
                     MDATGMALGDYVAELNIYSNDPDDPQVTIPLTMHVVDIAVNLTASDEDICLGESVEIT
                     SAMTGGSGSFTYTWTSEPVGFTSSEQNVTVTPEVTTTYFLSVFDGTYTVQDQVTIQVN
                     PLPVTFLGEDQTICVGDSVTLDAGAGFASYLWNTGDTTQTITAKITGEFSVSVTNEFG
                     CSANDAMTLNVIQTVDKPVITGGDGTVDNYQLTSTVYTCAAVEGASAYQWLIEPANAG
                     TTSSTGVSAEVFWTAGYTGTVNITVVASNTCFTSEPSDAFTTTVYSSAGLGENISGTQ
                     VSIYPNPTDGNISVRIPALKTFSGDLTLTDANGANVFNKTGVVIAAGDAATIDLGQLP
                     KGMYTLKLSSKTDTFYGKVVIR"
     gene            complement(18701..>20555)
                     /locus_tag="PKZ74_01960"
     CDS             complement(18701..>20555)
                     /locus_tag="PKZ74_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91543.1"
                     /translation="QELLAVHNDVLIGGAATFRVHTEADALVALSIDGEIIGVADGTG
                     EPQDIAVAAQQPGVVVDIVITKPNRFRYESKITVADPNTAYVVYDNYLIQDDAGNGNG
                     SADYNEEISLDLSLINLGLMDAENVSVTVSSDDPYVAVTDPEEQAGDVPAGEVVTVNG
                     GWTCRIADSVPDQHKIFLNVAAEDGSDIWHSEFFITALAPDFDLISHYIDDPQGNNDG
                     KLDPGETATLHMTIRNTGSSAAYDISAGLGPNDYYLSVLSDYQFAGDLEAGQETTVVF
                     TVAASELTPGGFNSMVNLNIEAGFNRYKTETFTLVIGDYSALVLDLDPMRNSGPAFVQ
                     TFDELGLFADYKTTIPSNLSDYSMVFVCLGIYFTNHVLTENEAQMLKNFLEDGGNVYM
                     EGRLTWYNDPQTSLQPMFNISPKFTNFYRIDTVYNIPGTFSNGMQFAYEMTQPYNNHL
                     LHAVEPATEIFKLLPDSLPAMVAYEAPGYKTIGTNIEFGGLVNGTNPSTRRELLLRML
                     QFFGQNLTSVNDDQIVTENRKASVSVYPNPFSREVNFEIKMDEPGQVRLEIFNVEGKP
                     VYRVFDGNLDSGKHIFNWNTDPYINSGSGNVYLFRLKTGSEIQGGKLFYLE"
BASE COUNT         5015 a         5121 c         5316 g         5103 t
ORIGIN      
        1 cggaaaacag agagggcgga gagagcattt cgcaatatca ataacaatga caggatcaat
       61 agtgttatta aagattttgc acagaatccg gagatgattt catcgtatcc tgaaaagtta
      121 taccgcgacg gagaatacca gtacctgatc attacgccgg aacagtttgc cccgtcgttc
      181 caggctctta ccgacctgta cagggtcagg ggatacatca ctgaaatcgc tacgaccgag
      241 tcgatcgacg cgaccatgcc cggccaggat ctccaggaaa agatcaggaa ctatattatc
      301 gctgctgttc aggccaacgg agtggagcat gtcctcctcg gcggggatgt ggaacatatc
      361 ccgcaccgcg gtttctattg ccatgtccag tcaagctccg tttacgaaga ctggaatatc
      421 ccggccgacc tgtattacag cgcactcgac gggaactggg acaccgacgg cgacggaatt
      481 tggggcgaga tcggcgaaga tgacctgctg ccggatgtgg cagtgacccg gatgcccatc
      541 agcaccagca ctcaactgca acggatgctg aacaagacca tcatgtacca ggatcagccc
      601 gttgccggtg aaatgagaga tattatttct gcaggcgaga acctctggtc ggatccgctc
      661 acctacggag aagattacct ggaactgctt ttcggatggc acgacgataa cggttatgtt
      721 accgacggca ttcctgagga ttacgatgtt aaccggctct acgactcgca aatgtactgg
      781 gataaataca cgctgatgaa cgccatcaac caggggagca gtttcatcca ccattcgggc
      841 catgccaacc agacctatgt gatgcgcttt gatatgtcgg atatcagcaa cgaaaatttc
      901 tcgcaagtca acggtaccga ccacaattac gctttgatct atacgcacgg atgcttatgc
      961 ggcgctttcg acgagagcga ctgcatcggg gaacggatgg tgctgatcga taacctggca
     1021 gtggccggcg gattcaattc gcgttacggc tggttcaacg aagggcagac ggaaggcccg
     1081 tcggcccacc tgcaccggga atttgtagat gcgctttacc acgaaaaaga atggcggatc
     1141 ggagccgctc atatgatatc caaaatagag acctcggttt gggtgaatgc acccggccaa
     1201 tgggaacagg gtgccttgcg ctggtgcttc tacgattgca atatcttcgg cgatgcttta
     1261 ctcggcgttt ggaccgaaga gccggtgtcc atcgagacca cttacccgga ggagatctac
     1321 ccgggcgaga ccacggtgca ggtgagcatt gcgaacggcg acgggcccgc cgtgggcttt
     1381 aactgtgtga ttatgtctgc cggcgaaatg attggctgcg cgactaccga cgaatcgggc
     1441 caggccgaga tcagtgtgcc cgaaggtttc atcgaccacc tggatgccca gctcattgtt
     1501 tcaggctata actgtttgcc tcatacctac gatcttaaca ttgttgttgg aattgatgag
     1561 ccttatgtga ttgattatct taatattatc ccgaatccgt tcagagatca gacatcgctg
     1621 gtttaccaac tcgaagaggc cggaagtatc gaagtaactt ttgtaaacct ggcgggtcag
     1681 atgatcgaaa aaatgcaatt cgacgggaag aaagggataa acagattcaa tatcaatact
     1741 acggaatggc cggaaggaat gatcatttcg aagattaccg gcgaaaaccg caccgattat
     1801 tatccactcc tgcacatcaa aaattagcgg agaattattt tgaattttct cttttttttg
     1861 atataatttt gtaattattc aaattcagga ttctgcatat gaaagaatcc tgaattcaac
     1921 ataacgatta caattatgaa caggatactt ttactttcat cttttatatt attgatgatg
     1981 agcggtctgg atgcccagac ctaccgttat gacgacagtt tcggcccgca gggtttcagc
     2041 ctggtttcgc acgaccggct gggtgttacg gtgaacttct ccatccggga attttccatg
     2101 gaagagatgg atatcaaagg cgacaggatg caacacatca gtcttccggg gcattttctt
     2161 cccggtgatg aagggactcc cgatctgccg ggcgacgggc gctttattgc cgttccaaac
     2221 ggggcaaaac ccgtgatctc ggtgaaaacg gccaggcggg aagtgatccg caatgtggat
     2281 atagccccgg caccgaacct gccgctggat aatgacgacc gaccggtcgt ttataaaaag
     2341 aacgcaaaga tttacagcac cgacgcctat ttcccggcgg agccggttaa attatcgggg
     2401 aagatgagta tccgcggcgt ggatgcattt gtactgggga tcacaccatt ccagtataac
     2461 ccggtaaagc gcgagctcgt tattttgagg gatatggaag tggagatcag gtttgaaggc
     2521 ggcagcggtg atttcgggga agaacgcctt cgcagccgtt ggtgggatcc gctgatgcag
     2581 gatatgttcc tgaactccgc cgatcttccc gttgttgaat attcgaagag ggtaacctca
     2641 ccgaacaggg ccaccggatg cgaatacctg atcatctgcc ctgacggtga agaatttata
     2701 gcctgggccg atacgatccg caggttcagg aaccagcagg gtatcctgac ggaggttttc
     2761 accatatcgc aggtgggtgg caacaacgcc atgatcatcg aggactttat caataacgcc
     2821 tattattcgt gggagattcc gcccgccgcc tgcctgctcc tgggcgatta cggttcattg
     2881 cctcagaatg ccatcacttc gagcctgctg aacgatcatc ccgacgatta caatccttat
     2941 atttccgaca atccgtatgc cgatgtgacc aacgacctgc tgcccgatat cgcttttgcc
     3001 aggatcacgg cccggaatgc ggctgaactg gagctgatga tcgggaaagc gttggattac
     3061 gagcgaaacc caccgaccag cgaatatttt taccatcacc ccatcaccgc gctcggctgg
     3121 cagacggagc gctggttcca gatttgctcc gaagccgtcg gcggctattt ccgaacgaaa
     3181 ggcaaagagc cgatccggat caatgaggtt tacggcggcg accccgatgc cgatccctgg
     3241 tcgattgccg ggaatactcc ggccatcctc agcacgttcg gcccggaggg gctggggtac
     3301 ataccggcta caccgggaga gctcggcaac tgggaagggg gcaatgccaa cgatatcaac
     3361 caggctgtaa acaacggcgc ttttcttctt cagcaccgcg atcacggaaa cgagaacggc
     3421 tggggggaac cgtactatac ccgcaatgag atcagcggtt tacaaaatac cgaactgact
     3481 tttgtgatgt cgatcaattg ccagaccggg aagttcaatt ccggaagcga gagcttcggg
     3541 gagaagtttc accggtacca gttcaatggt gaggccagcg gggctttggg cctgatcgcg
     3601 ccgacagagg tctcttattc ctttgtgaac gatgtttttg cctggggatt ttacgacaac
     3661 ctgtttcccg atttcttccc tcagttcggc acaacgccgc cttcgagagg tatgtacccg
     3721 gctttcggca atgccgccgg caaatatttt ctatttgcct cggggtggcc cagcaatcct
     3781 cagcataaat cgatcactta caagttgttc catcatcacg gcgatgcttt caccggattg
     3841 ttttcggagg ttcctcaagc acttaccgta gctcacgatt cggtaatgct ggccgggact
     3901 tttttcttcg atgtagaggc tgatgaagga tccctgatcg ccctttcggt gaacggggaa
     3961 ctgatcggtg tggctactgc cacaggagag ccggttcccg tagagatcat tccgcagaat
     4021 cctccgtata ttctggacgt agtggtgacg aaggagaacc acttccgcca ccattccact
     4081 gtgcagatca taccgcccca ggggccattt gtcataagcc aggcctgcca tgtgaacgat
     4141 acgcagggga acggcaacgg gctgctggat tacggtgaaa cagccgtctt cgatatgact
     4201 ttcgacaacc tgggcaacga gagcgccgag gatgtcgtcg tacatattta tactgacgac
     4261 gaatatgtta cgatcattga taatgagacc tttgccggaa ctatcgcggc cggcgaggtg
     4321 aaagaggtag aggatgcttt ttcagtgacc cttgctgata atgttcccga cggccatgtt
     4381 gtacattttg agatggaagc gaccgacggc gactcaatct ggatcagcac atttgctgtt
     4441 aagacccatt caccggtact ggtgtatgcg gagtatgcag ttgctgatac cggcggaaac
     4501 ggcaatggcc ggctggatcc gggcgaagaa gtcgggatcg tcatttccct gaagaataac
     4561 gggactgctt ccgcttacga tgtctacggt ttgctttcca gcaccgatcc ttatattgag
     4621 atactatccg attcggtcat gtttggtgaa gtgggaccgg gcgaaacggt taccggcgct
     4681 tttaccgcta cagcctatgt tatcaccatg cccggccatg aagccggatt cgatatcgat
     4741 ttttcgggta acctcgggat tgcagcagca gatgattttt cgctgttcat cggccttttc
     4801 cccgtgctga tcctcgacct ggatcagaat cacaactctg ccgacaaaat gaaggctgcc
     4861 cttgacgact tgcgcatatt ttccgagatc ctcgatgaga tgccggaaga tctgagccag
     4921 tacaagacca tctttgtatg tcttggaact tacagcgata atcatatcct gaccgccgac
     4981 gaagccggaa agctcatcgg tttcctgaat ggcggcggga atctttacat ggagggcgcc
     5041 gatacatggt attacgatca attgtattcg cctaccgagc tccatccgat gttcaatatt
     5101 accggagaag acgacggaag caacaatctt gtcaccgtca acgggatcac gggtactttt
     5161 acagaagggt tatctttctt tttcagcggt gataataatt atatcgacca tatcgtaccc
     5221 ggagaaggcg cttattccat tttcaccaat tcaaacccgg tttataacct ggccattgtt
     5281 aatgatgccg gtaattacaa gacgatcggc acctcttttg aattcggtgg cctgatgaat
     5341 aatctgaaca gttcacggaa ggacctgatg ctgaagtatc ttgacttttt cggtatggat
     5401 gcgattactc agggcccgct gttgcctgtt ggcgacacgc tggcgtgttc cggatcttca
     5461 tccggtattt atacaacgca tcccgttcct gacgctcatt actatatctg ggagctgttg
     5521 ccggccagtt ccggaacgat caccggatgg gatacgacag taacggtgga gtggacacct
     5581 ggatttgaag gaacggccac cctgagagtc tgcgggatga acgagaacgg ccttggaccg
     5641 ctttcccccg gcctggccat ccatcatttt acttcgcctg cggcaacagt tgcattctct
     5701 gatccggtca tatgccccgg cgacactacc ttcatgaaca tcacgctgac cggatcgcaa
     5761 ccctggcaga tgctgatcag cctgggagga taccagatca atctcaatcc caataagccg
     5821 atcatggacg gcatcccgct cagccccacc gccgacctgg aggtgaccat acttgccctc
     5881 tcggatgcta ccggttgcat tgcaacggga ttcccttcca atacgttaca ggtgacccag
     5941 atacccggca ttccggccat tccggcgggt cctgtgtatg ttgacctcta cggggcaaca
     6001 caaagtgagt acgcggtggc cgatacaatg cctgctaata actatgaatg gatcctgctg
     6061 ccgcaggagg ccggtaacct tacattaagc gacgacggga ctgtatgtac cgttaactgg
     6121 acggccggat ttaccggtga ggctacattg aagatgaggg gtgtgaacga atgcggcgag
     6181 gggagtttct ccgatccttt gaccgttaat gtgatcaatt catccggtgt taaagatgga
     6241 aacgtcggac cggccatttc ggtttttccg aatcccagct ccgggtcatt catcattcag
     6301 attaactcca atgctgacat tagcgggatt cttactttaa ccaacagttc cggcaggaag
     6361 atattggaaa cggaaaagat caactttagc tacaatgccc ggattcccgt tgacgcccaa
     6421 ttcttatcac cgggtatcta ttttattgaa attaaaaccg ataaaactgt ttaccgggat
     6481 aaagttgtcg tcaaataacc ttattctgat tccgtgatta acaatatccg ggaaggttat
     6541 ttaaaatgtt gataaataac aacatctccc cgaattaagg atattattaa taattttacg
     6601 gccgaaaaat cgatttttaa ttcattaatc ttttacacta aatgaaagct aatctgaaca
     6661 ttatcagaat agccgcatgg tttattatag ccgggctatt cctgatcaat cctgtttttt
     6721 cgcaaaaaat cacctattcc gatcccctaa acaaagccgg attttctctt gggcaggaaa
     6781 cccgcggggg tgttacggta aattacacta ttgaaacttt cacgttctca cctgttgata
     6841 tcaatggtaa ggggatgaag aatgtggagc tgccgggaaa cttcctgccc ggtgatgccg
     6901 gtgcgccaaa cctgccgggc agcgggcgtt atattgcttt tccaaaaggt gcggtaccgg
     6961 ttctgaccat tgtatcgcag cgtaccgagt cgtttaagaa cgttgatatt gcaccatcgc
     7021 cggagatacc gctcgatacg gaccgggaac cgctcaaata ccctgtaaga actgacattt
     7081 atactaccaa tgcatattat ccggccacac cggtgcagct ctccaacccg actcaaatca
     7141 gaggggtgga tgtggtgatg ctgggtgtta cccctttcca gtataatcct gtaacaaaag
     7201 aactgatcgt ttaccgtgac ctggagatcc gggtcgattt ccaggggggt agcggacaga
     7261 tcggtgaaga gaggctgagg agccgtttct gggaccccat cctggaggat gccgttctga
     7321 atcacaaaag cattcccgcc gttgattaca gcaggcgcgt tctgaatccg aacaggagca
     7381 acaacggggg tgattacctc attatcgttg ccaacagcga cgattatatg aattgggccc
     7441 aggagatcag gtcgttcagg ataaagcagg gtattgtcac cgaaatcagg acgctggaag
     7501 atgtcggagg aaacagtccc gcgattatcg agaattttat cgatgatgtt tatgaaaact
     7561 gggatccggt cccggccgca atattgctga tgggtgacta cggcagcaat gccgaagtga
     7621 atatcacttc cccgatctgg gacagctatt gtgtttcgga taatatctac ggtgatgtgg
     7681 atgaggatga tatgcccgat attgtaatgg cgagaatggt cgggaacaac aacaccgaaa
     7741 tggagacttt cgccaccaaa tttatcaatt atgaaaccaa tccgccgaca aatccctcgt
     7801 tctatgatca tccgatcaca gcactgggat ggcagacgga acgctggttc cagatctgtt
     7861 ctgaaacggt gggaggctat tttaagaatg tgcagggaaa gaatcccgtc cggatcaatg
     7921 agatttacct gggcaacccg ggttccgatc cctggtcgac tgcaaccaat acgcaggtta
     7981 ttctggatga attcggtccg aacgggcttg gttatattcc ggcaacaccg caggaactgg
     8041 gaaactggtc gggtggcaat gctacagcaa ttaataacgc gatcaacagc ggcgccttcc
     8101 tgcttcagca ccgcgatcat ggcgaggaga ccggctgggg agaaccgcaa tacggtaatg
     8161 gcaatattgg ggggctgacc aacaccgacc tcacctttat tttttccatt aactgcctga
     8221 cggggaaata caactggtcg ggagaatgtt tctcagagaa attccaccgg tacaggtata
     8281 acaatgtgaa cagcggcgcc ctgggagtca tttgcgcttc cgaagtaagc tactcatttg
     8341 tgaatgatac ctatgtttgg ggattgttcg acaacatgtg gcccgatttt atgcccgaat
     8401 acggttcgac gcctgcagag cgggggcttc ttcccgcttt tggcaatgcg gcaggcaaat
     8461 actttttaaa agcttcgagc tggccctaca attctgagaa caaagaggtg acttacaacc
     8521 tgttccacca cttcggcgac gctttccaga ccctctattc ggaggtgccc caggagatga
     8581 ccatcctgca cgacggcata atcctggccg gtctgaatga atttaccatt acagccgatg
     8641 ccgggtcaac catctgcctg acggtcgatg acgatattat aggcctggca accggaacgg
     8701 gtaatccgca ggtaataacc attaccccgt tatcggtagg aaccacggtg acgctgacgg
     8761 taaccaagac caactacttc cggcatgaag aggacatcga ggtgatccct gccgaaggag
     8821 cttactgcct ctacggtaac catactgtgg ctgatggcat tggcggtaac ggcaacggcc
     8881 tggtggatta caatgaaacc gtcggcctga gcctgggaat ccgcaatgtc ggtatgtcag
     8941 acggcgatgg tgttacggta accatctcta ccctggatcc ctttatcgct attgaagata
     9001 atacagaatt ttacggagtt attccttccg gacagaccgt ggttgtagat caaggatttt
     9061 ctttctatgc cgcagagaac accccggacc agcaccaggt ccttttcagc atagtttcaa
     9121 cggatggtgg aaatgtctgg aatagccagt ttatgctgaa agttaacgct cctgtactga
     9181 atatcaatgc ggtgacgata aacgattcgg aaaacggcaa tggcaacgga attctcgatc
     9241 cgggcgaaca ggcagagatg acggtgaatt acaccaacac aggacatgcc acggcctatg
     9301 atgtggatgt gttcatggaa ggtcaatcgg gatatattga gatttcaaac ccggagcaaa
     9361 atttcgcctc gataggtttt ctgggggttt ttaataaaac ctttgatgta tcggtcgatg
     9421 agataacccc gatagggatc aatatcaatt ttgtcaacga cctgtcgatg ggtaattttt
     9481 attcttcgaa gactttcatt cagaaagttc atccgctgat cgaagatttt gagaccggga
     9541 atttcaatga atatgactgg cagtttacgg gcaccttacc ctggacaaca agcaaccagt
     9601 acccgtatga ggggaatttc agcgcattgt cgggtgcgat ctcgcatggg caaacctctg
     9661 agatgaagat ttcatacgag gtaatgtcgg ccgatagcat ttccttcatc cgcaaggttt
     9721 cttccgaagc aaccgacaaa ttacagttct atatcgggaa cacgctggcg ggagaatggt
     9781 cgggaacaaa cgaaggctgg aggcgtgagg cctttgccgt aacgccggga tacaagacgt
     9841 tccgttgggt ttataccaag aatgcctccg ggagcggtgg cgctgacaaa gcctggctgg
     9901 ataatatcca tctgccttca ccggtagcgc taaccatctg ggccggcccc gatgaagata
     9961 tctgcaccgg cgaagagttc cagatctcag gttcttatgg cacggattat aacaccattg
    10021 aatggacaac cagcggtaca ggcacattta cggataatac ccagatgcag ccggtatact
    10081 cgcccagctc agaagatttc aacaacggaa gtgttgttct gacgctcacc ctgacaggtg
    10141 acggggattt aatcgtatcg gatgaaatgg tgctgacctt caacgccggc ccggaagccc
    10201 cgcaggccat tgaaggcccc gattatgtcg acctttttat taccacgaca tctgagtacc
    10261 tcactgtggg cattgaaggc attacggtgt atacctggca ccttgagcct gctgacgccg
    10321 gtacaattga aggatcgggc ctccagtcaa cggtgacctg ggatccggta tttgccggga
    10381 ctgcttatat cagtgtttcc ggtaacgatg aatgcggcga aggtgatgtt tcggaagctt
    10441 ttgaagtgac tgttggaaat tctgtgggtt actcagaaga tgagaacagc ttcgggctgt
    10501 cggtttatcc gaatcctggc aacggcagtt tccaggtgat gattgcaact ccggaacaga
    10561 acgctgtgac cctgaaagtg atcaatattc tgggcgaagt ggtatataag tcgaatcaga
    10621 aagtgaacgg tgccgccatt atcccggtga atattgagaa cctggccaac ggtgtctatc
    10681 tcttatccct ggaaggagat ggagtctcat tgaacagaaa gatcgtcaag aggtaaaaat
    10741 atcaaatctg aaactgaaga ttgggtgtct caaaagttct tcgtgcaact tcgtgaaacc
    10801 ttcgtaaaat ttataaataa ttgttacaca gagttccacg aagaaggcac taagatatac
    10861 gaagtttttt gaaagaaatc ttactttttc agacaccctc ttttttgggg gggtagtccg
    10921 acgactgaag ccgtcggttc ccaccttctt aaaaccattg gaaccaacgg ctttagtcgt
    10981 tggagttcga tcacaggcaa tagacgcctt tagttatcga attgcccgga aaagcgtcgg
    11041 actacaagta aatatgatgc catgcagaca aataaaacat tattctgctg acagttattt
    11101 tatgacgatt cattttatta caacctgcaa acggccgagt attttatttt ccttatcgct
    11161 gatggtgatg tagtatgtgc cggctggctg tgctgaaaga ttgaaactct ttttaatgac
    11221 gcctttaccg agcttttctc cggtttccaa tactttgttc cctccggctg atgctacgct
    11281 gaagatcagt tcctgttcct cttttgaatt catttcaaga acaaaattac cttcgtttgg
    11341 attcgggaat agcttgaccg aagagatggc cccaggctcg ccaataccct gtgatgaata
    11401 aacagaaacg ctataaacct gcgaatcatt tccattcccg caatcgttga caccttttgc
    11461 cgttatatca gccgtaccgg taaaacctgt attccaggtt acctgggcgg ttgtcccgtt
    11521 gccatcgaca gaacctgcgg cagccgggga gatattccat tcatatgaat cagcataagc
    11581 gctttcggca agggtataat ccgaaacaag tatgacgtaa agatcgacag actgaggccc
    11641 ggaggttatt tgcggggcca atggaagcgg gtccattatc agggtgacgg tatcggtatt
    11701 cttacagccc gtctcatcag taatttccac ccagtaatcg cccggcaggc cggtatcaat
    11761 ggatgcggaa gtagcgccat tgctccagag gtagccttcg tagccgcttc cgggagtcag
    11821 taacaccgtg gtgccttcgc agaaaatccg gtcttcgccc agatcaactt ccggccgggg
    11881 aagaacggcc agcgtgatcg tgtccctgtt acggcatccg ttattatccg ttacttccac
    11941 ccagtattcc ccgggttcgc cggcattgat gtagtagctt gaagcgccag tattccaaag
    12001 gtacgactgg aacccggttc ctgcggatag catcaccgtc gtcccctggc agaactgctg
    12061 gtcgggaccg agactgattt cagggagagg attgaaaata acattcaccg tatcggtggc
    12121 agcacactca ttttcattga gcaccgtgac ccagtaggaa cccggaccgg taaccgtaat
    12181 cgattgagcc gtttcacccg tactccagaa gtactgctga aaaccggcac cggcatccag
    12241 tacgacttca tcatttacgc aggcaaacag atcattgccc aggttcaggt cgggaatggc
    12301 cagtacggta atggtaatct ggtcggatag aatgatattt ccatcggtag cttcaacaaa
    12361 ataggttgta gttacagtgg gttcaataac aggatccgga tcatccgata cgaaccctgc
    12421 cgggtcggag gtccaggaat aggtaaaatt acctgatcca ccggtaatat tggcatgcag
    12481 agtggttgaa ctgcccaggc agaggctgtc tttatcgctg gtaatggtca ccgctgcatt
    12541 atccacgtgc atcgtgaccg gaaccaccag ttcgccgttg tccggatcat tgctgatgat
    12601 ggtaaagtgg gccaggtaag ttcccagggc gagccctgcc gaattaaaat caaaagtaac
    12661 cggctgggtt tcacctgcgt caatttcccc ttccctggga tttgcggtca cccatcccag
    12721 gacattgagg gagtaatcct cgacttcacc atttggcgca aagccacagg ggcttaatgt
    12781 gcctcctctt ctgatcctga tcctcattcg gacctccccg ttggcggcat tatccggaac
    12841 ggtaatcgtt gccgtgtaag gcccggggcc cggagatccg gataccgtga tggcgccatc
    12901 gtcaagaaag ctctcatttc cgttccagtc gacccagata ccgcatacat cctcagaata
    12961 catgttcgtt atattttgta ccgtaatctc aaaagaatcc ccgagtgaaa ccaggtggct
    13021 gaggtcggaa taatccgaat agctgttaca ttccgagaaa ttgctgacat caccgtaaac
    13081 aacaccggca attccttctc ctccgcagcc tccgattccg gtacaatatg ccaggccggc
    13141 ttccctggca ttgccgggaa ggctgaaagt cgaggtatac tccaacggaa gttccccggt
    13201 attggccagg tccatgataa aagaagaggt tgattcagga aggacaaata cttcgatgga
    13261 agccggatcg gtctctgcct gggcgtcgcc ccactgaaca tcggcaatag ccggagccga
    13321 ttccccttca ataatataga cagccgtaac ctggtacctg tacgacccat agccgggcaa
    13381 catatccgaa tagatgggca ataccgtggt tccgatcaat gaaaagccgc ggtaaatatt
    13441 gtaatgatca aatgtagggc ctgcttcatg cgtccaggtg aggttaacct gcccggtttc
    13501 atcgtttagc tgtgcattca gggcggttgg cggcatcatt gtgctggtca catatggcga
    13561 agtatatgcc agcgctgtgc ctgaacggtt atcggtccag acagggtaaa cctttccatt
    13621 cctggctgat atacccaggt aatctccaaa atactggctg gcataaccgg gaaggggaga
    13681 aggggagaaa gccacatcac tgactttaaa atcttcccag gtatctcctg cgtcgatgga
    13741 gttagcacag aaaacctcga cctgtgagct gctcacgttg cggtcgtcgt aaaagatcat
    13801 gctgagcgtc cctgtagcag gatcacaggt gatccatccg aaatagtgct ttttccctgc
    13861 gccctgcgga tcctggttga cgcggatcgg ctccgaccac gtgtcgccat tatcttctga
    13921 acggatcata taaacatcga catcgttgcc ggtattaacg cccggtaccc cgacattggt
    13981 ccaggtcaca taaatgttgc cccggttcgg gccgctgctg atatcacagg tcattaccgg
    14041 gaatgcattg accctcatgt tcttacctgt gcccgtgctc ctgatgcccc tgatgttcgt
    14101 aataatccgg aaggattccc aggtctggcc cccgtcgaat gaacgggcca ttccgatggc
    14161 atcttcatcc gaaggccagt tgtcgtaaat gcagaatact gcgtaaactt cgccgttagg
    14221 tccggtatta atgttaacac cctggcaaag gtaaccggca ttggccccgg cgctgatttt
    14281 caccggtgtt gaccacgact ggccgtcgtt tgtcgaccgg ctgagttcaa tttcgctgtc
    14341 attcggcccg ccgaaaggtg tccaggcgtc gtacagggtg ccttcatacg ggctggttgg
    14401 agagttatcg atccacatat ggttcttatc cagggtattg cctccgccgg aggataccgt
    14461 cacaggggtc caggtcgctc cctggttatc cgaataagag actccctggc ccattccgtt
    14521 gttgatatat ccgatgtaat atcttccgtt caggccaatc gctgtggtcg gatccccctg
    14581 gttcgtcccg ccggcgcctt gtaccgaccc ttgccatgtc aatccctcat caaaagaata
    14641 aaggtaatcg gagccgtaaa aggtcatccc tccccccggc tgtgtactgg agttattgga
    14701 gttgagcgga ttattggcat cctgggggtt gacaaaaaca gaattttcac tttgtgccgt
    14761 attaccggtt ataatgacca catcgggaga atcttccgta atgacggaaa cggccctgat
    14821 cccgcttccg gtataaacgg cgggcggtgc gctattcatc ggattaagcc gggtgaggcc
    14881 cagttcggcc gctttgcgcc agtagccctg gttatcgata gccgtgttca gtttcttaaa
    14941 acgatcagga acctgtgcat aagatgaaac tccggtcagt aagctgagta gaataactgt
    15001 taatgttaac tgtgatttca ttgagttaaa tgttaaggtc atcacaaaat taagcatact
    15061 ttggcggaac tggccaaaaa aaaagaggct gtcaggatcc tggcagcctc ttttatacta
    15121 ttcagaagaa tattatctga tcacaacctt accgtagaag gtatcggttt ttgaagacag
    15181 tttcagggta tacattcctt tgggtaactg accaaggtcg atggtcgctg cgtcgccggc
    15241 ggcaataacg accccggttt tattgaaaac attggcgcca ttggcgtcgg tcagggtcaa
    15301 atctccgctg aaggttttca aagccggaat cctgacagag atatttccgt cggtcggatt
    15361 cgggtagatg gagacctggg ttccggagat gttttcaccc aggcctgcgg atgaataaac
    15421 cgtagtggta aatgcatccg aaggttcgct ggtaaaacag gtgttgctgg ccacaacggt
    15481 gatattgacc gttccggtat agccggccgt ccagaacact tctgcgctga cgccggtgct
    15541 ggaagtggtt cctgcattag ccggttcaat cagccactgg taggccgatg cgccttccac
    15601 cgcggcacag gtgtaaacgg tagaagtgag ctggtagttg tcaacagtgc catctccgcc
    15661 ggtgatgact ggtttatcga ccgtctggat cacattaagt gtcatcgcgt cgttggcaga
    15721 acatccgaat tcattggtaa cgctgacaga aaactcaccg gtgattttgg ccgtgatggt
    15781 ttgagttgtg tcacccgtgt tccacaaata actggcaaag cccgctccgg catcaagcgt
    15841 aacggaatcg ccgacacata tggtctggtc ttcaccaagg aaggtgaccg gcagcgggtt
    15901 gacctggatg gtcacctggt cttgtaccgt ataagttccg tcgaaaaccg acagaaaata
    15961 ggtagtggtg acttccgggg taacggtgac attttgctcg gaggatgtaa atccgaccgg
    16021 ttctgaggtc catgtgtaag tgaatgaacc cgatccgccg gtcatagccg atgtgatttc
    16081 tacgctctcg ccgaggcaga tatcttcatc gctggctgtc agattaacgg caatatcgac
    16141 cacatgcatg gtcagcggaa tggtcacctg cggatcatcc gggtcgttgc tgtaaatgtt
    16201 gagttccgcc acataatcgc cgagggccat accggttgcg tccatggtta cggctatttc
    16261 ggcgctttcg ccagccggga tggttccttc aagcgggttt acggcgagcc agctctgcag
    16321 gaataccgaa taatcttcgg tttcgcccca gctaagtgct ccgcagggca ccggcgtcat
    16381 gttataaacg atccgggcgc ggagcctggt ttcgccggcc agagcgccca ccggcggggt
    16441 gatcgtcgcc gtgtaggggc ctactcctgg tgaaccgtta accggaatcg actcattatc
    16501 ttcaaactct tcgttctggt tccagtcgac ccataacccg cactggtcgt cgggatagct
    16561 caggttaccg ttataaatgg tgatttcata agaacttccg accgacatca tcgtggaaat
    16621 attcgtaaag ttactgtaac cgagtccttc aacacattcc gactggttgt caatgtcgtt
    16681 cagtgttacc ctggcgatgt attcatcgca ctgagccgtg gccgtgcaat acgctttcgg
    16741 atcctggcca gctttggtcg ggatgaacat accgatcgta taatccatcg gtaactggcc
    16801 tatgttgttc accgtcacat aattggttac cgtattgtcg atcatcagga actcttcgat
    16861 ctccatcggt ccggcactga tttgcgcatc gccccagcgg acattgacag aattggtgcc
    16921 gctggattca ttaccgtcgg catatttggc ggtgatccgg tacacgtaat ccccatatgc
    16981 aggcaactgg tcgttgaaga ttgtatcggt cgccattgct atggaatcat tgttacggta
    17041 tattttaaaa tagctgaatc cttccatctc ttcataagcc cattgcagat cggtgatacc
    17101 ggtttcaaaa gtcacactgg cggtaagatt ggtcggtttg ttgatcgggt tggtctgata
    17161 aggagagcaa tagctcatga caatacctgt acggttatcg gcccataccg ggtaaaccca
    17221 tccgccgcgg gctgaaatgc ccaggtagtc gcccatgtaa ccgtcggcca gtccggggat
    17281 cggggatggt gtgaacgcca catcgctcac tttgaaatct tcccatgttt cgccgccgtc
    17341 aaaagagttg gcgcagtata cttcgcactg ggtgctgccg acattgcggt cgtcatagaa
    17401 aacaacgctc agcgccccgt tttccggatc gcaggtgatc caggggaagt aatgtttctt
    17461 tccaagtccg gaggcatcct ggttcacttt caccggggtc gaccatgtca ggccctggtc
    17521 ggtcgagcgg ataaaataga catccacatc acttccggag ttaatcccgg gaacaccgat
    17581 attggcccat acaatgtaaa gattgcccct gttttcaccg ttgctgatgt cgcaggccat
    17641 gctggggaag gagttcttcc tcatattttt aacaatactg gtagtacgga ttccacggat
    17701 gttattgata atcctgtatg atggctcgta gcttgcgccg ccgtcgaagg agcgggccag
    17761 gcccatggag gattcatccg atggccacga atcgtaaata gcccagagga cgtaaacctc
    17821 gccgtttggc ccggtctgga tgttcacacc ctggcagtgg ctgcctgcat tgacggcgct
    17881 gctgacattg atcggggtct ggtaagccag cccttcattc gtcgagcggg ctacctcgat
    17941 aggccagccg gatgctccga agtcggtgta cgcaacataa aggtttcctt catgcggact
    18001 ggtggggctg ttgtcgatcc acaggtggtt tttgtcaagg aggtcaccgt tttgagtggc
    18061 agccgtgata cgggtccagt tgacgccgtt atcggaatag gaaaccgatt ggccgtagtt
    18121 attatcgata aatccgatat attgcctgcc ggtgaggctt atggccgttg ccgggtcgcc
    18181 ggagttgctc ccgccggcgc cgttcaccga accgccccag gtcagggcgc cgtcttccga
    18241 aaggaaataa ttggcgccgt aaaggtttcc aacaggattt tgggttgaat tgtttgaatt
    18301 caggaccatc tggttgtttg tcgggttgac aaaaatggag ttttcactct gggttgaatt
    18361 cacactggtc accgggacat cgggggagtc ttcggtgatc gtcgataagg cacggatttc
    18421 actgccggta taaaccgccg gctcaaccga tctgaccgga ttgagcgtag tcagccccag
    18481 tttggccatc tccttgtaat accggttaca gtcgacccgt gtattgacta tcttcccaag
    18541 cctgggatca tcaaggggtt ttaataccaa cgtctgaccg aacattgcgc cactcgtcag
    18601 aataagagcg gtaagcatca gaatgtaaat ttttttcatg ctgtttttgg tgtatagtga
    18661 taagattgaa ttggaattac ataaggttgt aaagataata ttattccaga taaaaaagtt
    18721 ttcctccctg aatttcactg ccggttttta accggaaaag atatacattt cctgatccgg
    18781 aatttatgta aggatcagta ttccaattaa atatatgctt tcccgaatcc aggttgccgt
    18841 cgaaaacccg gtaaaccggt ttcccttcca cattgaatat ttccagcctc acctgcccgg
    18901 gttcatccat ctttatctca aaattcacct cccgggagaa aggattggga taaacggata
    18961 cggaagcttt tcggttttcc gtgactattt gatcgtcatt gacagatgtc aggttctggc
    19021 cgaagaactg cagcattctc agaagcaatt cccggcgggt cgaaggattt gtgccgttca
    19081 ccagcccccc gaactcaatg ttcgtgccaa tggttttgta accgggagcc tcataggcca
    19141 ccatggccgg caatgaatcg ggcaacagtt tgaagatctc ggtggccggt tcgacagcat
    19201 gcaggagatg gttattataa ggttgcgtca tctcataagc aaactgcatc ccgttgctaa
    19261 aggttccggg gatattgtaa accgtatcaa tccggtaaaa gttcgtgaat ttgggtgaaa
    19321 tattgaacat cggctgcagg cttgtctgtg gatcgttata ccatgtcaga cgaccttcca
    19381 tgtaaacatt tccgccatcc tccaggaaat tcttcagcat ttgtgcttca ttttccgtca
    19441 ggacatgatt ggtgaaataa atgcccaggc agacaaagac catgctgtaa tccgaaagat
    19501 tggaggggat agttgtttta taatcggcaa acaggcccag ttcatcaaat gtctgaacaa
    19561 atgcgggtcc ggaattccgc ataggatcga gatcaagaac aagcgctgag taatcgccga
    19621 tcaccagggt aaatgtctcc gttttgtacc ggttgaaacc ggcttcaata ttgaggttga
    19681 ccatggaatt gaagcccccg ggcgttaatt cgctggcagc aacggtgaaa acgacagtcg
    19741 tttcctggcc ggcctccagg tcgccggcga actggtaatc ggaaagcacc gaaagataat
    19801 aatcgttcgg gcccaggccc gctgatatat catatgcagc cgatgaacca gtgttcctga
    19861 tcgtcatgtg gagcgttgcc gtttcaccgg gatccagttt gccgtcgttg tttccctgcg
    19921 gatcatcaat ataatggctg ataaggtcga aatcgggcgc cagggctgtg atgaaaaact
    19981 cggagtgcca aatgtcgcta ccatcttctg cggcaacatt gaggaatatt ttatgctggt
    20041 cgggaacgct gtcggcgatc cggcaggtcc atcctccgtt taccgttaca acctctccgg
    20101 caggtacatc accggcctgc tcttccggat cggtcaccgc aacgtaagga tcgtcggatg
    20161 atacggttac gctcacattc tcggcatcca tcaacccaag gtttatcagc gacagatcca
    20221 gcgaaatctc ttcattataa tcagccgatc cgttgccgtt tccggcatca tcctgaatta
    20281 aataattatc atatacgaca taggccgtat tgggatctgc cacggttatc ttcgattcat
    20341 aacggaaacg attgggtttc gtgatcacga tatccacaac aacgccgggt tgctgcgctg
    20401 caacagcaat atcctgcggc tcccccgttc catcggccac cccgatgatc tccccgtcga
    20461 tactgagcgc taccagtgca tctgcttcgg tatggacccg gaatgtggcc gctcctccga
    20521 ttagtacatc gttgtgaaca gccaggagtt cctgc
//
LOCUS       DAOPNH010000018        19638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2648_length_19638_cov_6.273962, whole genome shotgun sequence.
ACCESSION   DAOPNH010000018 DAOPNH010000000
VERSION     DAOPNH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_19638_cov_6.273962"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKZ74_01965"
     CDS             complement(<1..1070)
                     /locus_tag="PKZ74_01965"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPM91544.1"
                     /translation="MKRPGGIINRAGLIAVLLLLLCYTINTADAQHVMRHKLRSPGLV
                     LEGRVYYGWTLDHHIEMTPYKRHYPGFELSLFKATYGRTRWEYMYNYPFIGVSYWYSD
                     LGGTKPLGSAHALFPFINFPLLRSRDANIYFRLGVGLGYLTKKYERYDNYDNLAIGSH
                     FNGAINLMFEVRGRLTERLMGSAGVSLVHFSNGGTKQPNYGINMPGVSVALAYRMSRE
                     NPYLRKKLLPELYPFEFDGKRYLQGDINMGFGFKDLKATSGKGHQYPVVDLAANVMWP
                     VGYKSRLGAGVDFSYDGADEKQLQKQNIIPEHRIDLMKTGINAAYELAFSRMAIMLNL
                     GFVVSGLDKHDGTVYEKLGIRVG"
     gene            1182..1967
                     /gene="mazG"
                     /locus_tag="PKZ74_01970"
     CDS             1182..1967
                     /gene="mazG"
                     /locus_tag="PKZ74_01970"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPM91545.1"
                     /translation="MKSAVDAFEKMLGIMDELREKCPWDRVQTLESLRHLTLEETYEL
                     SDAIMEKDLDGIKKELGDLMLHIVFYAKIASEMKAFDMADVLQSINEKLVRRHPHIYG
                     DVQVKDAGEVRDNWEKIKLTEGRDSVLGGVPHSLPALVKAYRIQEKASGVGFDWDNAG
                     QVWEKVVEEMNEFRREVDCAEEDCDKQHLQQEFGDLLFALVNYGRFLEINPEDALEQT
                     NRKFIRRFQYVEKGAKRSGKFLHQMSLEEMDVFWNEAKKQENQ"
     gene            complement(2017..3069)
                     /locus_tag="PKZ74_01975"
     CDS             complement(2017..3069)
                     /locus_tag="PKZ74_01975"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM91546.1"
                     /translation="MPEDLPEIPDSQEGQEESQDLYEHHRFVVDKGQGLLRIDKFLMF
                     RIENATRNKIQQAAHAGNILVNGNAVKPNYRVKPQDVISVVLSYPPREIELIPENIPL
                     RILFEDEDLIIVDKAPGMVVHPAYGNYSGTLVNALTYHLREVSSDRAPGRTPFLVHRI
                     DKDTSGVMLAAKNELAQARLAAQFFHHTIDRHYTALVWGDFDTDSGTISGHIGRSVRD
                     RKLFTVYIDASQGRHAITHYSVMERFGYVTLVDCKLETGRTHQIRVHFQHIRHPLFGD
                     ETYGGNVILKGTTFPKYRQFVNNCFEILPRQALHARSLSFKHPTTQKIISFESAPPPD
                     MQEVIEKWRRYSSSKF"
     gene            complement(3074..5218)
                     /locus_tag="PKZ74_01980"
     CDS             complement(3074..5218)
                     /locus_tag="PKZ74_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91547.1"
                     /translation="MKKFFIIFFVLLFFSLAIRAQEIITELQENPVVRAEHDRMMANG
                     SRESAGETAPVMLPFFDDFSGIGIYPDSKKWIGEEAFLNTNYGYRMANIGAVTLDAID
                     SRGLLHANASHFPFQADSLTSRPIRLDSIFLPTPRKITVADSIYLSFYYQPQGRAYQA
                     PESTDSLILQFGYSTGDSVFANYYDSIWVQLSDYIHPEDTIFPGDTIFSPAGCADVYV
                     IADNYYYYNDLMRMPCDSVFLPEFRWQRIWSASGTTLAEFYEKYNTYSKQVMIPITDS
                     SRYFKNDFSFRFINFASLASENNPSWRGNCDQWNIDYVYLNINRSYRDTVYRQVSFVE
                     RPPSMLKRYEAMPYNQYTNNPGNEMKNELELVITNLDSTIFNSTYYYVVNRTEGDFQY
                     IYPGGNCNLFPFNLNGYQNCIACAQHACPPVNFVFPLDVTDSAEFTVRHIIIGDITPT
                     DTVSDTIYYAQKFFNYYAYDDGTPEAGYGLNLTPSGAKLAYRFTLNVKDTLRAIQMYF
                     NHTQNDANVKFFDLMVWQDNNGKPGEVIYSQLDEKVEFSSELLGFHTYMLDEPILVNG
                     TFYIGWQQQTPDNLNLGYDRYNNAQQQIFYNTSGEWLQSINTGALMMRPILGKPFDLS
                     SVPDNKTFENRITVYPNPLNGTRISFKGDRILTEDYHGLSISVINSLGTEIVRMPFSN
                     PADTGPLSPGLYLIAIKDENGRIVSVTRLIKN"
     gene            complement(5218..6234)
                     /locus_tag="PKZ74_01985"
     CDS             complement(5218..6234)
                     /locus_tag="PKZ74_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPM91548.1"
                     /translation="MAINLRKSILTVLTDHSILYKNTIFATLNAITGWIYVIIRQAAL
                     TPAWRNQLTMGALSFLVKKNFYIHFGISVILTIALLLIVISLLKVYTRHGEAYIVPDL
                     TGKEYDRLFEIEETNVFQFIVTDSVFDNSLLPGSVIKQNPSPGSKAKEGRTIYLTIVS
                     YTPKMSRMPELKDLTVRQAVTTLRTGGLKIRKLVFTPHFAGNSVLGQYFEGDTLLAGT
                     EILEGSEIDLLAGLGENRSIRVPIVMGLTRDEARSALQMASLNAGKEHYLDRKDPMHS
                     RVYRQFPSWQEDLYPGDSVTLYFRSDLTYNFDDLLNSVNPDTAVFLDLEVEMPTDSLE
                     PEID"
     gene            6335..7264
                     /locus_tag="PKZ74_01990"
     CDS             6335..7264
                     /locus_tag="PKZ74_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91549.1"
                     /translation="MSFYKSIGSISFRNFIRITPVLLLILNALSVTSQGYTKDVRSYQ
                     SDDKVIIQYRLKVTNKKGYVDYNHRMKSRRFDISLFHSYDQGNQYHSSGNVKGKVGKN
                     IHAGQGKRIIWRPDSEFGYIGNIQFKVEANPRIPKVCWGLSYSRGIYNFGTLRSPEIN
                     RIHLKIYKIGKGNGSTFFPLGISTGNTRVIQDSYIPDSSYILIEIKKTLSKALTVGIG
                     GQTRNRAFSIYVAAGVMIQDEDIEKFKEESDNILVDAGKEFTSDLKFILDGGISIKLF
                     DTKPFTVLLPVNVFWEPIDQDYISINTGLEVQF"
     gene            7848..8723
                     /locus_tag="PKZ74_01995"
     CDS             7848..8723
                     /locus_tag="PKZ74_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPM91550.1"
                     /translation="MVFKTPDFRTGTTEQLDTFKIQGDITVDMDIPTTRMSQPNSIAI
                     IIGNRDYRYVSPVEYAINDAESIKDYLVNTLGFKESNILFKPNATKSDFEEYFGNQEY
                     FEAKLYNLVKPGISDVFIYYSGHGAPGLKNHKVYFVPVDCDPSYLEFKGYGLETFYQN
                     LARVPARSFTVVLDACFSGEDIHKNISAVLVKITNPVFSLNNGILLASSASSEPSYWY
                     HDQQHGLFTYFFLRAIKERKISDKDRNDKLTYQEVFDYISSQTDGIPYYSRRLYGKAQ
                     NPTLQGPSDRVLVVY"
     gene            8777..9766
                     /locus_tag="PKZ74_02000"
     CDS             8777..9766
                     /locus_tag="PKZ74_02000"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPM91551.1"
                     /translation="MEKKRYIIAIAAGGDSGEYEISLKSAAVVKKHLDPGRMESYLVI
                     IRGNDWKISLEDGTTWPVDRNDFSADLPGRTIKFDGVFIAVHGTPGEDGKLQGYFEML
                     NIPYTSCDSLTSALTFDKFFCNHFAKSFGVNVSRSIVINRGDDIPEKEILTRINLPFF
                     VKPDRGGSSVGTSKVKTPDQLAAALQMAFREDSRVMLEEHIPGRELSCGTITYKGKIM
                     TLPITEIISRKEFFDYEAKYEGMSDEVTPADIPDHVAREISDTSVRLYQLMNCRGMVR
                     FDYILNDTRLFFLEVNTVPGMSELSIVPQQAMKAGISLKDLFTETMLGALNAR"
     gene            complement(9729..10319)
                     /locus_tag="PKZ74_02005"
     CDS             complement(9729..10319)
                     /locus_tag="PKZ74_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HPM91552.1"
                     /translation="MKELVFATNNAHKLKEIRAIAGDTLRILSLKEIGCTDEIVEDAA
                     TIEGNASLKAFYIWNKYGKYCFADDTGLEVEALDGRPGVKSARYAGDDCNPDNNIKKL
                     LKELLDKPNRKARFRTVISLIADGREVQFEGIVEGEILPEKKGKDGFGYDPVFRPIGY
                     EQSFAEMSPDEKNRISHRGRAVQKLIAHLMRPASFR"
     gene            complement(10316..11005)
                     /gene="ung"
                     /locus_tag="PKZ74_02010"
     CDS             complement(10316..11005)
                     /gene="ung"
                     /locus_tag="PKZ74_02010"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPM91553.1"
                     /translation="MQTVNPRIEESWKLVLQEEFSAPYFQDLTEFLREEKQKYTVYPP
                     GTQIFSAFDHTPFNRVKVVILGQDPYHGPGQAHGLCFSVPDGVTHPPSLANIFKEIRN
                     DLGFPVPSSGNLTRWAEQGVLLINAILTVRADTPTSHQNKGWELFTDAVIRNLSEKRK
                     NLVFLLWGNYAQAKEKLIDDSRHFILKAAHPSPLSASRGFFGCRHFSETNKILSDLGL
                     KEIDWRLEEQL"
     gene            11122..11250
                     /locus_tag="PKZ74_02015"
     CDS             11122..11250
                     /locus_tag="PKZ74_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91554.1"
                     /translation="MKKFIIAIIALGLYALAMTACSSSTDCPAYGEVHKYQIEQRY"
     gene            11331..12086
                     /locus_tag="PKZ74_02020"
     CDS             11331..12086
                     /locus_tag="PKZ74_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91555.1"
                     /translation="MKLFPLIILVGIIALSFHSVYAQEELMEDYDSIAERILLKKEMT
                     GGATLHTLGMGANFRKGVNKSYFTSRIFEVEVVSMKHPKQVKVIAYYTAKSFVYGKLN
                     HVYMLRAGYGYKKLLNRKPYWGGVELRALYMGGASIAFAKPVYLYFYDLNYQYRYEEK
                     YDPEKPAHSSEFIYGRAPFTDGIWETKIYPGVYAKGALNFEFGKLNTKIRALEVGGVV
                     EFFPIAVPIMAYRPKHNVFATFYLNFSFGKRYN"
     gene            complement(12063..>12259)
                     /locus_tag="PKZ74_02025"
     CDS             complement(12063..>12259)
                     /locus_tag="PKZ74_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91556.1"
                     /translation="KAVGSSNLKDSLPTPKAVGSSDLKDSLPTTKAVGSSDIKIKEPT
                     ALVVGLGTCHQTVLIIPFPE"
     assembly_gap    12260..12359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12649..14940)
                     /locus_tag="PKZ74_02030"
     CDS             complement(12649..14940)
                     /locus_tag="PKZ74_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91557.1"
                     /translation="MKRIFTTLIFLSLVGFLSLSAQTRVYTPELSQPVNGAVDQMPDA
                     VLDWNAVTGGNTGVILYDVQLDDDPAFGSPANFQTELVSAYKTANLGFGQTYYWRVRA
                     RDGNDVSDWSAAWSFRVIRRVVLKSPTDASTQNTDLTLGWNPITGLTQYEYQFDTSYY
                     WNTMSSGITTILYDVATVDETHAWAVGAGGKVLFFDGNSWTAQTSSVTVDLYDVHFLD
                     VNNGWAAGKSGKIIHYDGTQWAAMTTNTSEQVNGITMLSPTSGWAVTKDSAILYYNGA
                     NWAEQYKAAKEMTKIIAVDASHVWAVGKGGVILFYNGSSWTAQQSGTAKDILDAAFVS
                     PDYGWAVGKTGTLIEYKNGTWVPFVNSLTNNRDVNSIKFNSPTSAFAVGKTGLMLQFD
                     GIEWYNQSSGTATTLNGVSMAGTTGFLVGESGVIVSYNDEAFTSPLATIHPVAGDITT
                     TNLIDLLFGEQYYWRMRAKHSQSTSDWSGVRSFITINTVELDKPPNNTTNADLDIELK
                     WKKVSNLVTYEIQIDDDVNFGSPIFLASSGIAINAELLKFGTAYNWRVRALHAFDQSD
                     WSAPWKFTTVNAVTLESPANNSTDIKLSPLLTWKKITGIAGYQVMVADNNGFNNPLAT
                     AVVDAAENSFIVPLVLDKDASYFWKVRAINGLDTSNWSPAWTFKTMPPVGIGEPTFEN
                     SIEVFPNPADDIAFIRFNGKGNATLQLTLSDIVGMKVMEKTLNYGEGLRIQSIDVSGL
                     KKGIYLVRLTHGNSAMTRKLIIK"
     gene            complement(14985..17972)
                     /locus_tag="PKZ74_02035"
     CDS             complement(14985..17972)
                     /locus_tag="PKZ74_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91558.1"
                     /translation="MNKRPLPFGYFILTMALSAFLISATLTGDEKIEARQETESDYQA
                     RIRNNQLTGQITPADYLKAQQQIRELAANRSGGAYDFDWQLLGPNNLGGRTRAILFDN
                     RDATGNTVYAGSVLGGLFKSDNQGSKWYKIDEANGGLYVSCITQAGNGDIYVGTGEGF
                     TVQQYTVFGEWRYSGGLMGQGIFKSTDGVNFTRLASTNPTINGNNELEWGFINELAAH
                     PSNGALYASTNTGLKYSSDGGNNWQAAKMADGTILNLSSGDVKMAANGLVVAEVSNKC
                     YVSENGNFDNFQLRSGKASFNLPDTSAIGRIEFAIAPANNDIIYALVVKTSGALYNVY
                     RSDDKGATWSVVGPGGSANFNVFNTGTASATGNGLFAATIEVYPDDPYHVLIGGYDLW
                     EGKKIQDDGFYQWVLRSTSSANWLSVAFLWQGHHVYRFAPGSSEKVLIGTNGGISLGL
                     LDPEFFQFQFMNKDYIASQFYTVSPTMEKKDVVGGAQDIGTIYIRGSVNPADSRRGED
                     IWTTQSNVPDGRTGGYCAWSTIYPTAVIYSRFPQPEGSPETFIRRNEFSGGPDWALNN
                     TFTTAYLSTSFLPPFVLYENFEDYNSKDSTEFKANRDLAAGTTVWVESNNGKRPFKHV
                     LEAAIDSSETVMVKDIISTRFFIGGDNRVMMTKQMIQFDVSPEWFTISDSLRGGVRGK
                     PQAMAYSSDANHLFVGTQKGKLYRISNIKYAYNNETADVSSPYCVISTKQIPVYLPGT
                     TTEITQVITSVAVDPNNDEKVIITLGNYGNETYVYYTENALDENPVFHSAQGNLPQMP
                     AYSSLIEMDAANHLVFVGTEHGIYVTNDIGSSNPTWTAENANIGAVPVFMLKQQTIRK
                     TNDTLVFINIDTTYLVHYGTNNYGVIYGATYGRGLISLDEYQKPVSIQEPKKINRNHE
                     IEVYPNPARERVTVGFESTSPAEIVVRIYDLNGKTVKHIDLGTRPQGRQDVIVSCQDI
                     PSGTYIMQVSIGRKVSSAKFIVQ"
     gene            complement(18238..18999)
                     /locus_tag="PKZ74_02040"
     CDS             complement(18238..18999)
                     /locus_tag="PKZ74_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91559.1"
                     /translation="MKRSAITVLTILLALLAQAQPAAISSLVRNGNEAYTAGDYKGAV
                     EQYESALRSGFESAELYFNLGNAYFKADDIPSAVLFYEKALRLDPSDENIHFNLALAN
                     SRITDKIEPLPEFFLKSWWNGIRDTFSSNEWASAGIFTFLILLAAVAIFIVSGSVMVR
                     KVSFWTGVILLMFTLTSFLFSLNSYHEYSRQSSAIIFTPTVTVKSSPTDGSVDLFVIH
                     EGTKVFITDRVDDWSEVRLVNGNVGWIKTNSFRPI"
     gene            complement(19007..>19638)
                     /locus_tag="PKZ74_02045"
     CDS             complement(19007..>19638)
                     /locus_tag="PKZ74_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protein BatD"
                     /protein_id="HPM91560.1"
                     /translation="QSYATLTTPAYGIKVLKGDNEPSGVVYSGVSQKNIQFIGSDIRH
                     IKTGQIPLHPVNSFLISSTALLLWLVVPALLFTAGLIFWARYKKQQGDIALVRNRKAT
                     KVARKNLKKAGEYLEAQNTEAFFEEVSRAMWGYISNKFNIPLSDLSIESVSQRLAGKK
                     VSSENILMFTEVLENCEFARFAPGDKPGRMQEIYNSALNVISRIEQELK"
BASE COUNT         4963 a         4521 c         4778 g         5276 t
ORIGIN      
        1 cctaccctga tcccgagctt ctcatatacg gttccatcat gtttatccag tcccgataca
       61 acgaatccca ggttcagcat gatggccatg cgggaaaaag ccagctcgta agcggcatta
      121 atccctgttt tcatcaggtc gatcctgtgt tcgggaatga tattctgctt ttgaagctgt
      181 ttttcatccg caccatcata agaaaaatcc accccggctc ctaggcggct tttgtagccg
      241 acaggccaca ttacattggc tgccaggtca acgacaggat actggtgccc cttgcccgac
      301 gtcgctttca agtccttgaa accgaagccc atattgatat ccccctgcag atatcttttg
      361 ccgtcgaact caaaaggata aagctccggc aataatttct tcctgagata aggattttcg
      421 cggctcatcc gataggctaa ggccacgctg actccgggca tgttaattcc ataattcggt
      481 tgtttggtgc ctccattgga gaagtgcacc agcgacaccc cggcagagcc catcagacgc
      541 tcggtcagtc ttccccgcac ctcaaacatc aggttgattg ccccgttaaa atgggatccg
      601 atcgcaagat tgtcgtagtt atcataccgt tcatatttct ttgtcagata acccagcccg
      661 acgcccagcc ggaaatagat gtttgcatcc ctgcttctga gcaacgggaa atttataaaa
      721 gggaataaag catgcgccga cccgagcggt tttgttcctc ccagatcaga ataccagtag
      781 gatacgccga taaacggata attatacatg tattcccacc gggtcctgcc ataagtggct
      841 ttgaacaggc tcagctcaaa accgggataa tgcctcttgt aaggggtcat ttcgatgtga
      901 tggtccaatg tccatccgta atatacccga ccctccagaa ctaatcccgg gcttcgtagc
      961 ttatgccgca taacgtgctg agcatcagcg gtatttatag tataacacaa caacagtaaa
     1021 agaaccgcta tcaggccggc ccggtttata attccaccag gccttttcat ttcaatcctt
     1081 ttccggtttg ttaacatatg tggacaaaaa taataaaata cgcgccaatg ccgggtgacc
     1141 ggcaggttca attcgttgta cttttgaaga aaattaccgt tatgaaatct gccgtcgatg
     1201 cttttgagaa aatgctgggc ataatggatg aactcaggga gaaatgtccg tgggaccgtg
     1261 ttcagaccct ggaaagcctc cgtcatctaa ctttggagga gacctatgaa ttatcggatg
     1321 ccatcatgga gaaagacctg gatggcataa agaaagagct gggcgacctg atgctgcata
     1381 tagttttcta tgcaaaaatt gccagtgaga tgaaagcgtt tgatatggct gatgtgctgc
     1441 aatcgatcaa cgaaaagctg gtacgccggc atcctcacat ttatggcgat gtgcaggtga
     1501 aagacgccgg cgaggtacgg gataactggg agaagatcaa actgacggaa ggccgtgatt
     1561 cggtgctggg cggggttccc cactccctcc cggccctggt caaagcctac cgcatccagg
     1621 aaaaagcttc aggggtggga ttcgactggg ataatgccgg acaggtgtgg gaaaaggttg
     1681 ttgaagagat gaacgaattc cgcagggaag tggattgcgc ggaggaagat tgcgataagc
     1741 aacaccttca gcaggaattc ggcgacctgc tcttcgccct ggtcaattat ggccgtttcc
     1801 tggagatcaa tcccgaagat gcccttgagc agaccaaccg taaattcatc cgccgtttcc
     1861 aatatgtgga aaaaggcgct aaaagaagcg gtaaatttct acaccagatg tcgctcgaag
     1921 aaatggacgt gttctggaat gaagccaaaa aacaggaaaa ccaatgaacc ccactttgaa
     1981 ctttgaactt tgaacttaga actttgaact ttgaacttag aacttagaac tactgtatcg
     2041 tcgccatttt tctatcactt cctgcatatc cgggggcggt gccgattcaa agctgattat
     2101 tttttgggtg gtgggatgtt tgaaggagag ggaacgggca tgaagcgcct gcctgggaag
     2161 gatttcaaaa caattattga caaactgcct gtatttcgga aatgttgtcc ctttgaggat
     2221 gacattgccg ccgtatgttt catcgccgaa taaaggatgg cggatatgct ggaaatgaac
     2281 cctgatctgg tgcgtgcggc ctgtttccag tttgcagtcg actagcgtga cgtagccaaa
     2341 ccgttccatt accgaataat gggtgatagc atgccggccc tggctcgcgt cgatgtaaac
     2401 cgtgaagagc ttccggtcgc gcacgctccg gccgatatgc cccgaaatgg tccccgaatc
     2461 cgtgtcgaaa tcgccccaga ccagtgcagt gtaatgcctg tcgatcgtat ggtggaaaaa
     2521 ctgtgctgcc agcctggcct gcgctagctc gttcttggct gccagcatca cacccgatgt
     2581 atctttatcg atccggtgca ccaggaaggg cgtccggccc ggcgccctgt cggacgagac
     2641 ctccctgaga tggtaggtga gggcgttgac cagggtgcct gaataattgc catacgcagg
     2701 gtgcaccacc atgcccggcg ctttatcgac aatgatcagg tcttcatctt caaaaaggat
     2761 acgtagcggg atgttctcgg ggatgagttc tatttccctt ggcgggtagg agagtaccac
     2821 tgagatcaca tcctgtggct tgacacggta attgggtttg accgcattgc cattgaccag
     2881 gatattaccg gcgtgcgctg cctgctgaat tttgttccgg gtggcatttt caatccggaa
     2941 catcaggaat ttgtcgatcc tgagcagccc ctggccttta tccacgacaa accggtgatg
     3001 ctcgtaaagg tcctggcttt cttcctgtcc ctcctggcta tccggtattt caggcaggtc
     3061 ttccggcatg gtctcagttc ttgatcaggc gcgtaacgga tactatcctg ccattctcat
     3121 ctttgatggc gataaggtac agccccgggg atagaggccc ggtgtcggcc ggattgctga
     3181 aaggcatccg gacgatttca gttcccaggc tgttgattac cgaaatggaa aggccatgat
     3241 aatcttccgt taaaatccgg tcgcccttga aactgatcct tgttccgttc aacggattcg
     3301 gataaacggt aatgcggttt tcaaaggttt tattatcggg aaccgatgaa agatcaaaag
     3361 gttttcccag aatgggtctc atcatcagag ccccggtatt gatcgattga agccattcac
     3421 cgctggtatt atagaagatc tgctgctggg cgttgttata ccggtcgtac cccaggttca
     3481 gattgtcggg ggtctgctgt tgccatccga tgtagaaggt gccgtttacc aggatcggtt
     3541 cgtcgagcat ataggtatga aaaccaagca gttcggaaga gaactctact ttttcatcga
     3601 gttgcgaata gatgacctcc cccggcttgc cgttattgtc ctgccagacc atcaggtcga
     3661 agaacttaac attggcatcg ttctgggtat ggttaaagta catctggatc gcacgaaggg
     3721 tatcttttac attgagtgta aaccggtatg ccagctttgc gcccgaaggc gtcaggttaa
     3781 gaccgtagcc ggcttccggg gtgccatcgt catacgcata ataattgaaa aacttttgag
     3841 cataataaat agtatcggaa acggtatccg tcggagtgat gtccccgata atgatgtgcc
     3901 tgacggtgaa ctcagcggaa tcggtgacat cgagcgggaa aacaaagttg accggcgggc
     3961 aggcatgttg tgcgcaggcg atgcaattct ggtatccgtt caggttgaat gggaagaggt
     4021 tgcagtttcc gccgggatat atatattgaa agtcgccttc agtgcggttc acaacataat
     4081 aatatgtgga attgaaaatg gtggaatcga ggttggtgat caccagttcc agttcatttt
     4141 tcatctcgtt gccgggatta tttgtatatt ggttatacgg catggcctca taccttttta
     4201 acatactggg cggccgttcg acgaaggaga cctgccggta gaccgtatcg cggtatgagc
     4261 ggttaatatt gaggtagacg taatcgatat tccactggtc gcagttccct ctccagctgg
     4321 gattattttc gctggccagg ctggcaaaat tgatgaaccg gaaagaaaag tcgtttttga
     4381 aataccggct gctgtcagtg atcgggatca taacctgctt gctgtaggta ttatactttt
     4441 cgtaaaattc ggcaagtgtc gttcccgaag cgctccagat acgctgccac cggaattcgg
     4501 gaagaaagac cgaatcgcag ggcatccgca taaggtcatt ataatagtaa taattatcgg
     4561 cgatgacata gacatcggca catccggcag gactgaatat cgtgtcgccg ggaaaaatcg
     4621 tatcttcggg gtggatataa tcgctcagct gcacccagat tgaatcgtaa taattggcaa
     4681 ataccgaatc acccgtggaa taaccgaact ggaggatcag cgaatcggtg ctttcagggg
     4741 cctggtaggc tcttccctgg ggctggtagt agaagctgag gtagatcgaa tcggcaacgg
     4801 ttattttcct cggggtggga aggaagatgg agtcgagcct gatggggcgg gaggttaatg
     4861 aatcggcctg aaacggaaaa tggctggcat tggcatgcag gagcccccgg ctgtcgatgg
     4921 cgtcgagggt aaccgccccg atattggcca tccggtaacc gtagttcgta tttaaaaagg
     4981 cctcttcccc gatccatttt ttactgtcgg gatagatacc aatgccggag aaatcatcaa
     5041 aaaaggggag cattaccggc gccgtttccc cggccgattc ccgtgaacca tttgccatca
     5101 tccggtcatg ttcagcccgt actaccggat tttcctgcaa ttcggtaatg atctcctgcg
     5161 cccggatggc caggctgaaa aataatagaa caaaaaatat gataaaaaac tttttcatca
     5221 gtcaatttca ggttcaagtg agtccgtggg catctccacc tcaagatcga gaaagacagc
     5281 tgtgtcggga ttgaccgaat tcagcaggtc gtcgaaattg taagtcaggt ccgaacggaa
     5341 atagagtgtg accgagtcgc ccgggtacag gtcctcctgc catgatggaa actgcctgta
     5401 aacgcggctg tgcatgggat ctttccggtc gaggtagtgt tcctttccgg cgttgagcga
     5461 tgccatttgc aatgcactcc tggcttcgtc acgggtaagt cccattacaa taggcacccg
     5521 gatggatctg ttttccccga gcccggccag caggtcaatc tcgcttcctt ccagtatttc
     5581 ggttccggca agcagcgtat cgccttcgaa atattgcccg agcaccgaat tgcccgcaaa
     5641 atgcggtgtg aaaaccagtt tgcggatttt caggccgccg gtccgtaaag tggtcacagc
     5701 ctgcctgacc gtcaggtctt tcaattccgg catccggctc atttttggcg tatacgaaac
     5761 gatggtcagg taaatggtcc ttccttcctt ggctttggag ccgggtgaag ggttctgttt
     5821 gatgacagac ccgggcaaca ggctgttgtc aaaaaccgaa tcggtcacaa taaactgaaa
     5881 gacgttcgtc tcttcaattt caaacagccg gtcatattct ttgcccgtaa gatcgggaac
     5941 gatatacgct tcaccatgac gggtatagac tttcagcagg ctgataacga tcagcagcaa
     6001 agcgattgtc aggatgacgg aaatgccgaa atgaatgtaa aaattctttt ttaccagaaa
     6061 gctcagagcg cccattgtca attgatttct ccaggcaggc gtcagtgccg cctgcctgat
     6121 aataacgtaa atccagccgg ttattgcatt gagtgtggca aagatagtat ttttataaag
     6181 gatggaatga tcagttaaga ccgttaatat tgattttctc aaattaatgg ccataaatta
     6241 tttttttata aatttggaaa gattaaccaa aaaagcaaac ccaaattagt atccgaagcg
     6301 aacccacaat agtatagcta aaactttgat cattatgagt ttttataaat caatcggaag
     6361 catttcattt cggaacttca taagaatcac gcctgttctt ctattgattc tgaacgcatt
     6421 atcagttact tcccagggtt atacaaagga tgtcagatcc tatcaatcgg acgataaagt
     6481 tattattcag taccggttaa aagtgaccaa taaaaaagga tatgtcgatt ataaccacag
     6541 aatgaaatca agaaggtttg atataagctt atttcattca tatgaccagg gtaaccaata
     6601 ccattcatcg ggaaacgtaa aaggtaaagt tggaaagaac attcatgcgg gtcagggtaa
     6661 gcgtatcatt tggcgaccgg attcggaatt tggatatatt ggaaatattc agtttaaagt
     6721 ggaagctaat cccaggattc cgaaagtatg ctgggggctg tcctattcaa ggggtattta
     6781 taattttgga acattgagaa gcccggaaat caatagaatt catcttaaga tatataaaat
     6841 cggaaaaggt aatggaagta ctttctttcc tttaggaatt tctaccggga acacaagggt
     6901 tatacaggat agttatattc ctgattcatc ttacatttta atagaaatca agaaaacctt
     6961 aagcaaagca ttgactgttg ggattggtgg acaaaccagg aatagggcat tttcaatata
     7021 tgttgctgct ggtgttatga tccaggatga agatattgag aaatttaaag aagaatctga
     7081 caatattctg gtggatgcag gtaaagagtt tacatcggat ttaaaattca ttctggatgg
     7141 tggaatcagc attaagcttt tcgataccaa accatttacc gttcttcttc ctgtaaatgt
     7201 cttttgggaa ccgatagatc aggattatat ttcgataaat accggcctcg aagtgcaatt
     7261 ttaaagtttg acttatgaaa tcgctgttaa ggataatatt tgttctgatt gtcggattat
     7321 ctggaaatcg tgcagaggca caaactacaa cgacaaatct gaatattctt cattattatt
     7381 tgatcaaatc aacaattgaa gacagagtca atcaggagat cggtgagtgg caaatgcaag
     7441 gcagatatga aaatactacc gattacaagg aaagagtgaa ttccaaaaac cgggaaaata
     7501 agattcacga attaacgatc aagcatatta atgtgattgc atcggaggtg gttcatttga
     7561 gtattacctc tttggagtat gatccggata atgaggtgta taagatatcc ctattcggtt
     7621 tgccgccggt ttatcttaaa gttcccgttg ataatttaca ggctaattac ttcgatagca
     7681 ataaaaatga attaagattt tcctcgccgg aatacacatt aaccgataca ggctttgcct
     7741 tattacgtac gacaatctat aatccgggga ataaaaggac atactattat aattaccagg
     7801 ataatctcat ctttaaacag aataaaatca gtattgaatt tgatccggtg gtatttaaaa
     7861 cccctgactt tcggacagga actacggagc aactggatac atttaaaatc cagggggata
     7921 tcaccgtgga tatggatatt cccacgaccc gcatgtcgca acccaacagc attgcgatta
     7981 ttatcggtaa cagagattac aggtatgtca gcccggtgga atatgcaata aatgatgcag
     8041 aatcgattaa ggattatcta gtcaacactt taggttttaa agaatcaaat attctattta
     8101 aacccaatgc cactaaatct gatttcgagg aatactttgg gaatcaggaa tattttgaag
     8161 ctaagcttta caatttagtg aaaccgggta tttcagatgt ctttatttat tattccggac
     8221 acggcgcacc aggcctaaaa aatcataagg tttattttgt tccggtagat tgcgaccctt
     8281 cctatttaga atttaaagga tatggtctgg aaacatttta tcaaaatctg gcccgggtcc
     8341 ctgcacgaag ctttactgtt gttttagatg cctgcttttc aggtgaagat atacacaaga
     8401 acatcagtgc tgttttagta aagattacga atccggtttt ttccctgaat aatggcatat
     8461 tactggcttc atcagcttcc agcgagccgt cctattggta ccacgatcag cagcatgggc
     8521 tctttaccta ttttttctta agggcaatta aagaaaggaa gatatccgat aaggaccgta
     8581 atgacaagtt gacttaccag gaagtctttg attatatttc ttctcaaacc gatggaattc
     8641 cctattattc aagaagatta tacgggaagg ctcaaaaccc gacactgcag ggcccatccg
     8701 acagggttct ggttgtatat taaggttccg gtatttcaat atctttgtac tctctgttgt
     8761 tatcttattt aattctatgg agaaaaagcg atatatcatt gccatcgctg ccgggggtga
     8821 ttccggggaa tacgagattt cgctcaagag cgcagccgtc gtgaaaaagc acctcgatcc
     8881 cggacgcatg gagagttatt tggtgatcat ccgtggaaac gactggaaga tctccctgga
     8941 ggatggcacc acctggcctg tcgacaggaa cgatttctca gccgatcttc ccggacgcac
     9001 aattaaattc gacggcgttt ttattgccgt gcacggtacg ccgggagaag acggaaaact
     9061 ccagggatat ttcgaaatgc tcaacatacc ttatacttcc tgtgattcgt tgacatcggc
     9121 gctgacattt gataagttct tttgtaacca tttcgcgaaa agttttggcg tcaatgtatc
     9181 cagatcgatc gttatcaaca ggggagatga tattccggag aaagaaatcc tgacgcggat
     9241 caacctgccg ttttttgtta aacccgatcg cggcggatcg agtgtgggaa cttcaaaagt
     9301 aaagaccccg gatcaactgg cggcagcgct gcagatggct ttccgtgaag attcccgcgt
     9361 gatgctggaa gaacacatcc ccggcaggga gctttcgtgc ggtactatta cttacaaagg
     9421 taagatcatg acgttgccca tcactgagat tatcagccgg aaggagttct tcgattacga
     9481 agcaaaatac gaagggatgt cggatgaagt gacaccggcc gatataccgg atcatgtggc
     9541 ccgtgaaatc tcggatacct ctgtcagact gtaccagttg atgaattgcc gggggatggt
     9601 caggtttgat tatattctta acgataccag gcttttcttc ctggaagtca acaccgtacc
     9661 gggtatgtcg gaactcagca ttgtccccca gcaggcaatg aaagccggta tcagcctgaa
     9721 ggatctgttc accgaaacga tgctgggcgc attaaatgcg cgatgagttt ctgaaccgca
     9781 cggccccggt ggctgatccg gttcttttca tccgggctca tctccgcaaa gctctgttca
     9841 tatccaatgg gccggaaaac aggatcgtag ccgaatccat cctttccttt tttttccggc
     9901 agaatctctc cttcgacaat cccttcaaac tgcacctccc gtccgtcggc aatcaatgag
     9961 atgactgtcc tgaaccgggc ttttctgttg ggtttatcca aaagctcctt cagcaacttt
    10021 ttgatattgt tgtccgggtt gcaatcatcc ccggcatagc gggccgactt cacaccgggc
    10081 ctgccgtcaa gggcttccac ttccagccct gtatcgtcgg caaaacagta tttcccgtat
    10141 ttgttccaga tataaaatgc tttcaatgag gcattacctt caatagttgc ggcatcctcc
    10201 acaatttcat ccgtgcatcc gatctccttc aggctgagga ttcgcaaagt gtctccggct
    10261 atagccctga tctcttttaa tttatgggca ttattcgtgg caaagacaag ctctttcata
    10321 gctgttcctc caatctccag tcgatttctt tcagtccgag atccgacagg attttatttg
    10381 tttcggaaaa atgccggcat ccgaaaaagc ccctgctggc cgataagggc gatggatggg
    10441 ctgctttcag aatgaaatgt cggctgtcgt cgatcagttt ttcttttgcc tgggcataat
    10501 ttccccagag cagaaatacc aggttcttcc ttttttccga aagattgcgg atgactgcat
    10561 cggtaaataa ttcccatccc ttgttctggt gtgaggttgg ggtgtcggcc cttaccgtca
    10621 ggatcgcatt gatcagcaga accccttgtt cggcccaccg ggtaaggttt ccgctggacg
    10681 ggaccggaaa gccgaggtcg ttcctgatct ccttgaaaat attggccagt gacgggggat
    10741 gcgtgacccc atccggtacc gaaaagcaca acccgtgtgc ctggccgggg ccatggtacg
    10801 gatcctggcc caggatgacc acttttaccc tgttaaacgg ggtgtggtcg aatgcggaga
    10861 atatctgcgt cccgggcgga tataccgtgt atttttgttt ttcttcacgc agaaattctg
    10921 tcagatcctg aaagtagggg gcagaaaact cctcctgcaa cacaagtttc cagctttctt
    10981 cgatccgggg atttacagtt tgcatatcaa attatttcaa atttcaggca atatatttct
    11041 ttctttaatg tttttaaaaa taaataaaat tatctttgcg atgataaagg tttgtcaaac
    11101 ccaatatttc gcccaaatat tatgaagaaa tttatcatag ccattattgc tttagggttg
    11161 tatgccctgg ccatgactgc ctgctcgtca tcaacggatt gccctgcata tggcgaagta
    11221 cataaatacc agatcgaaca aaggtattag tcgtttgttc tcttttctga ccgtcaattg
    11281 gcataatcat tgcgtattgc aatgggatgc gttggtcatt acttaaatct atgaaactct
    11341 ttccgctgat aatacttgtt ggtatcatcg cattgtcctt tcacagcgtt tacgcccagg
    11401 aggaactgat ggaagattac gacagtatcg ccgagcggat cctgcttaaa aaggagatga
    11461 ccgggggagc taccttgcat actctcggaa tgggcgcgaa cttcaggaaa ggggtcaata
    11521 aatcctactt cacttccagg atattcgagg ttgaagtcgt cagcatgaaa catcccaaac
    11581 aggttaaagt cattgcttat tacacagcca aaagtttcgt ttacggaaag ctcaatcatg
    11641 tatacatgct tcgggccggg tatggctata aaaagctttt gaacaggaaa ccttactggg
    11701 gtggtgtgga actgagggcc ctttatatgg ggggcgcttc cattgcattc gccaagcctg
    11761 tttaccttta tttttatgat ctgaattatc agtacaggta cgaggaaaaa tacgatccgg
    11821 agaaaccggc ccattcgagt gaattcattt atggacgggc gccgtttacc gatggaatat
    11881 gggaaacgaa gatctacccg ggcgtttatg ccaaaggggc cttgaacttt gaattcggga
    11941 aactgaacac caagatcagg gcgctggaag ttggcggagt cgtcgaattt ttcccgatag
    12001 ctgttcccat catggcatac cggccaaagc acaatgtgtt cgccacgttc taccttaatt
    12061 tctcattcgg gaaacggtat aattagaacc gtttgatgac aggtacccag tccaacgact
    12121 aaagccgttg gttccttgat tttgatatcg ctggaaccga cggctttagt cgtcggaagg
    12181 gaatccttca gatcgctgga accgacggct ttaggcgtcg gaagggaatc cttcagattg
    12241 ctggaaccga cggctttagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    12361 cggctttagg cgtcggaagg gaatccttca gattgctgga accgacggct ttagcgtcgg
    12421 aagagaaact ttcaatgaag aaggtgtctc aaaaggtaag atttctttca aaataacttc
    12481 gtgtatcttc gtgccttctt cgtggatctc tgtgtaacaa ttatttataa attacacgaa
    12541 gttccacgaa ggtttcacga agctgcacga aggacttttg agacacccac aagggaatct
    12601 gccacccggt cggaagggaa gctcacctta aatattgctc aaaatggact atttgatgat
    12661 cagttttcgg gtcatagcgc tattcccgtg agtcagccgg accaggtaaa tgcctttctt
    12721 caatccggaa acgtcaatgg attggattct gaggccttct ccgtagttca gggttttctc
    12781 catcaccttc attccgacaa tgtcggagag ggtaagctgg agcgtcgcat ttcctttgcc
    12841 gttgaaccga atgaaagcaa tatcgtctgc cgggttcgga aacacttcta tgctgttttc
    12901 aaaagtgggt tcgccgattc ccaccggagg cattgtcttg aaggtccagg cagggctcca
    12961 gttggaagta tcgaggccat tgatcgccct gactttccag aagtagctgg catctttatc
    13021 gagtaccagc gggacgatga aggaattttc cgccgcatcg acgacagctg tggcaagcgg
    13081 gttattgaat ccgttgttat cggccaccat aacctggtac ccggctattc cggtgatttt
    13141 tttccaggtc agaagcggcg acagcttaat atcggtcgaa ttgttagcag gtgattcaag
    13201 cgtaactgca tttacagtag tgaatttcca gggcgccgac cagtctgact gatcgaatgc
    13261 atgcaaagcc ctgaccctcc agttgtaagc cgtgccgaat ttaagcaatt cggcattaat
    13321 agcgattccg cttgatgcaa ggaagatggg tgagccgaag ttgacatcat catcaatctg
    13381 tatttcatac gtcacaagat tggagacttt cttccattta agctcaatat ccagatcggc
    13441 gttggtcgta ttgtttggcg gtttatccag ttcaacggta ttgatagtaa tgaaggagcg
    13501 aacccctgac cagtcggagg tgctctggga gtgtttggct ctcatccgcc agtaatattg
    13561 ctcgccgaat aagagatcaa taaggtttgt cgtggtaata tcaccggcaa ccgggtgaat
    13621 agtagccaga gggctggtga atgcttcatc gttgtaactg acgatcactc cggactctcc
    13681 taccaggaac ccggttgttc cggccatgct gactccgttg agcgttgtgg ccgttccact
    13741 ggattgatta taccattcta ttccgtcaaa ctgaagcatc agccccgttt tcccaacagc
    13801 aaatgcgctg gtgggagagt tgaatttgat cgaattgaca tccctgttgt tggtaaggga
    13861 gttgacgaaa ggaacccagg tgccattttt atattcgata agtgtccccg tttttccaac
    13921 tgcccaaccg taatccggag agacaaaagc ggcatccagg atatcttttg ctgtgccaga
    13981 ctgctgtgca gtccatgatg aaccgttata gaacaatatc acgccgcctt ttccgactgc
    14041 ccatacatgg ctggcatcga cggcgatgat ttttgtcatt tctttagcgg ctttatattg
    14101 ttccgcccag ttggctccgt tatagtatag gatagcactg tctttggtta cagcccagcc
    14161 gctggtgggg ctcagcattg tgatgccgtt tacctgttcc gaagtattgg tggtcattgc
    14221 cgcccattgt gttccgtcat aatggatgat cttgccactt ttaccggcag cccatccatt
    14281 attaacatcc aaaaaatgga catcgtaaag atcaacggtg accgaactgg tctgagctgt
    14341 ccatgagttc ccgtcgaaga atagaacctt cccgcctgct ccgacggccc aggcatgagt
    14401 ttcatctact gtagcaacat catacagaat cgttgtaatg ccggaactca tagtattcca
    14461 gtaataactc gtatcgaact ggtattcgta ctgggtaaga ccggttatgg ggttccagcc
    14521 cagcgtcagg tccgtatttt gcgtagaagc atctgtcggg cttttcagta caacccggcg
    14581 gatgacgcgg aatgaccatg ccgcagacca gtctgaaacg tcatttccat cccgggcccg
    14641 tactctccag tagtaggtct gtccaaatcc caggtttgct gttttatatg ctgataccag
    14701 ttctgtctga aaattggccg ggcttccgaa tgcaggatca tcatcaagtt gtacatcgta
    14761 taatatgacg ccggtattgc cgcctgttac ggcgttccag tcaaggaccg catccggcat
    14821 ctggtcaacc gcaccattga ccggctggct gagctccggt gtataaaccc ttgtctgtgc
    14881 cgaaaggctg aggaatccga ccaggctgag aaatatcagg gttgtaaata ttcttttcat
    14941 cattttattt ttttatatag gtgaaaatga aatttttagc ttagttattg tacgataaat
    15001 ttggcggatg aaaccttccg gccgatcgac acctgcatga tgtatgttcc cgatggaata
    15061 tcctggcaac tgacgatcac atcctgtctg ccctgcggtc ttgtcccgag atcgatatgt
    15121 ttgactgtct taccgttcag gtcataaata cggacaacaa tttctgcggg tgaagtgctt
    15181 tcaaatccaa ccgttacccg ttcacgcgcc gggttcggat agacttcgat ctcatggttc
    15241 cggttgattt tcttaggttc ctggatgctg accggtttct ggtactcgtc cagggagatc
    15301 agcccgcggc cataagtggc gccgtaaata actccatagt tgtttgtccc gtaatgcacc
    15361 aggtaggtcg tgtcgatatt gataaagacc agtgtatcgt ttgttttccg gatcgtttgc
    15421 tgtttcagca tgaaaacagg cactgcaccg atgtttgcgt tttcagccgt ccaggttgga
    15481 ttggatgatc caatatcatt cgtaacgtag ataccatgct ccgtcccgac aaacaccaag
    15541 tggttggcgg catccatctc gatcagggaa gaataggccg gcatttgcgg cagattaccc
    15601 tgtgcagagt ggaataccgg gttttcatcc agggcgtttt cggtgtaata cacataggtt
    15661 tcatttccat aatttcccag agtgataatt accttttcat cattgttcgg atcgactgca
    15721 acagaggtga tcacctgggt gatttctgtt gtagttccgg gcaggtacac ggggatttgt
    15781 ttggtggaaa tgacgcagta agggctggag acatcagcgg tttcattatt ataggcatac
    15841 ttgatattgg agatcctgta aagtttgcct ttctgagtgc cgacgaacag gtggttggca
    15901 tccgaagaat aggccattgc ctgcggtttg cccctgacgc cgccacgcag cgagtcgctg
    15961 atcgtaaacc attcggggct tacgtcgaac tggatcatct gtttggtcat catcacgcgg
    16021 ttatcgccac cgataaagaa ccgggtggag ataatgtctt tgaccatcac ggtctcactt
    16081 gaatcgatgg ctgcttcgag cacatgtttg aaaggccgtt tgccgttgtt gctttctacc
    16141 caaacggtag tgccggcggc gagatcgcga ttagctttga attctgttga atctttactg
    16201 ttataatcct caaaattttc atacagcacg aagggtggca ggaatgaagt gctcaggtaa
    16261 gccgttgtga atgtgttgtt aagtgcccag tcgggtcctc cgctgaattc attgcggcgt
    16321 ataaaagttt ccggtgagcc ttcgggctga gggaaccggg aataaatgac cgctgtcgga
    16381 tagatggtcg accatgcgca ataacctccg gttcggccat ccgggacatt gctctgggta
    16441 gtccagatat cttcgcccct cctgctgtcg gccgggttga cagatccgcg gatataaatt
    16501 gttccgatat cctgggctcc gccgacgaca tcttttttct ccatggtcgg gctgaccgtg
    16561 taaaactggc tggcgatata atccttgttc ataaactgga actggaaaaa ttccggatca
    16621 agcaatccca gggagatgcc cccgttggta ccgattaaca ctttttccga tgaacccgga
    16681 gcaaaccggt aaacatgatg cccctgccac aggaaagcca ccgacagcca gttggctgaa
    16741 gaggtggacc gcaggaccca ctggtaaaaa ccgtcgtcct ggatcttttt tccttcccag
    16801 aggtcataac ctccgataag cacatggtac ggatcatccg ggtatacttc gatcgtagcg
    16861 gcaaaaagcc cgttaccggt agcggaggcc gttcctgtat tgaaaacatt gaaattggcg
    16921 cttccgcccg ggcccacaac cgaccacgtc gcccccttgt cgtccgaacg gtagacattg
    16981 taaagcgcgc cactggtttt tacaaccagt gcataaataa tatcattgtt ggccggggcg
    17041 atggcaaatt cgatcctgcc gatggcgctg gtgtcgggaa gattaaaact ggctttgccg
    17101 gaacgaagct ggaaattgtc gaagttgcca ttttccgata catagcactt gttgctcact
    17161 tcggcaacca ctaacccgtt tgctgccatt tttacatcgc ctgacgagag gttcaggatc
    17221 gttccgtcgg ccatctttgc agcctgccag ttgtttccac catcggagga gtatttaaga
    17281 ccggtgttgg tggaagcata caatgcgcca ttcgaagggt gagcagccag ttcgttgatg
    17341 aagccccatt ccagttcgtt attgccgtta atcgtaggat tggttgacgc cagacgggtg
    17401 aaattcaccc cgtcggttga tttgaaaatc ccctggccca tcagcccgcc ggaatatctc
    17461 cattcgccga atactgtgta ttgctgaacg gtgaatcctt caccggtacc aacgtaaatg
    17521 tcgccgttgc ctgcctgtgt gatacagctg acgtaaaggc ctccattggc ttcgtctatt
    17581 ttataccatt tgctgccctg gttatcggat ttgaacaaac cacccagaac acttccggcg
    17641 taaacggtgt tacccgtagc atcgcggttg tcaaacaaaa tggcccgggt gcggccgccc
    17701 aggttgttcg gaccgagcaa ctgccagtca aaatcatacg caccgccaga tctgttggct
    17761 gccagttctc tgatttgttg ctgcgccttc aggtaatcgg ccggagtgat ctgaccggtc
    17821 aactggttgt tcctgatcct ggcctgataa tccgattcgg tctcctgcct ggcttctatt
    17881 ttttcatccc cggtaagcgt cgcagagatc aaaaaagccg acaaggccat tgttaaaata
    17941 aagtagccaa agggtaaagg tcttttattc atataaatag agtttgattt attgtaatcc
    18001 gtccttataa aaacagtcgc ccaatttaca ctatttttat atacgtgcaa atccggctta
    18061 gttaaaattt taacaaagat atttataata tcctgccctt gttaagtgat acaaatttaa
    18121 aattaaaagg ataagattgg tggtttgatc agtatccggc aagtgaaaat caggaaacgg
    18181 cctgtgaggt caggcctgcg taaaattgca aactgcctgt tcccgccgca gatcaagcta
    18241 aatcgggcgg aacgaatttg tcttgatcca gcctacgttg ccattgacca gcctgacttc
    18301 gctccagtca tccacccggt cggtaatgaa gactttcgtc ccttcatgga tcacgaataa
    18361 atccacactc ccatctgtgg ggctgctctt tacagtgacg gtcggtgtga atatgatggc
    18421 cgacgattgc cggctgtatt cgtgataact gttcaggctg aaaaggaagg aggtcagcgt
    18481 gaacatcagg agaatcacgc cagtccagaa actgactttt cggaccatga ccgaacccga
    18541 aacgatgaaa atggcgacgg ccgccagaag gatcaggaaa gtgaatatgc ccgcactggc
    18601 ccactcgttc gatgaaaagg tatcgcgtat gccgttccac cacgatttca ggaaaaattc
    18661 gggtagcggt tctattttat ctgtaatacg gctgttggcc agcgcaaggt taaaatggat
    18721 attctcatcc gaaggatcca gcctgagcgc tttctcgtaa aacagaacag cagacgggat
    18781 atcgtccgcc ttgaaataag cattccccag gttgaaatac aattcagccg attcaaaccc
    18841 tgaccgcaat gcgctttcgt attgttcgac ggctcccttg tagtcaccgg ctgtgtaggc
    18901 ttcattaccg ttccgtacca gtgagctgat cgccgcaggt tgcgcctgtg ccagcaaagc
    18961 cagcaggatt gtcaaaacgg ttatcgctga tctcttcatc tctttgttat ttcagttcct
    19021 gttcaatacg gctgattacg ttcagggctg agttgtaaat ctcctgcatc cgtcccggtt
    19081 tgtcgcccgg cgcaaaacgg gcaaattcac agttttcaag aacttcggtg aacatcagga
    19141 tattttcgct gctgaccttt ttgccggcaa gtcgctggct cacgctttct attgagaggt
    19201 ccgacagggg tatgttgaat ttgttgctga tatagcccca catcgccctg ctcacttctt
    19261 caaaaaaagc ttcggtattt tgtgcctcca ggtattcacc cgctttttta aggtttttcc
    19321 tggcaacttt cgtggctttc cggttgcgaa ccagggcaat gtcgccctgt tgctttttat
    19381 acctggccca aaaaatgagc ccggctgtga aaagaagggc ggggaccacc agccagagca
    19441 acaaggcggt tgaactgatc aaaaaggaat tgaccgggtg cagtggaatc tgtccggtct
    19501 tgatgtggcg tatgtcggac ccgatgaact ggatgttctt ctggctgact ccgctgtata
    19561 ccacacccga cggttcattg tcgcccttca gtaccttgat gccgtaagcc ggtgtggtta
    19621 atgttgcata gctttgcc
//
LOCUS       DAOPNH010000019        19135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2767_length_19135_cov_6.333229, whole genome shotgun sequence.
ACCESSION   DAOPNH010000019 DAOPNH010000000
VERSION     DAOPNH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2767_length_19135_cov_6.333229"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKZ74_02050"
     CDS             <1..599
                     /locus_tag="PKZ74_02050"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPM91561.1"
                     /translation="DVTWGINLSNIGSKISYSKQSTKKDFIPTNLRLGGGLKMNFDDY
                     NSLALLLDLNKLMVPTPPIYLRDSSGNPVIDPATNNPVIDKGKDPNVSPVAGIIQSWY
                     DAPHGFSEEMREWAIAFGMEYWYAKQFSLRTGFFYEDKTKGNRKFITVGAGLRYSVFG
                     LDLSYLIPFEQQHPLENTLRFTLHFNFDPDKESDLPRK"
     gene            complement(613..1374)
                     /locus_tag="PKZ74_02055"
     CDS             complement(613..1374)
                     /locus_tag="PKZ74_02055"
                     /inference="COORDINATES: protein motif:HMM:NF037852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91562.1"
                     /translation="MKNLVILSRLVILLFFSQNIFSQVAINTSGADPDPSAVLDINAP
                     DKGLLIPRVTLLSKVNNNAPIQNPATGLMIYNLGGNNLDAGFYIWNGMTWSALATVEQ
                     VMNLANAPQPAAVFGELYEYHNIGDFTTIQVASGGTWMPWNTAQEGDTSEMAASASTL
                     TILNPGTYTVSFNAALQMSSGGKITEAALFVNGIRQDDMRTRCWFKEGSKAQNFSFSG
                     LIELNEGDVVSVKFTVNENGTIRLEMANLNLTRLN"
     gene            complement(1457..2872)
                     /locus_tag="PKZ74_02060"
     CDS             complement(1457..2872)
                     /locus_tag="PKZ74_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM91563.1"
                     /translation="MTIQGEIFRNFPFEPTRGQQNLADELAKFIGDPEPWSVFILKGY
                     AGTGKTSFVSAMVRILPVIRKKAVLLAPTGRAAKVLSGYAGQPAWTIHKRIYFQRVGR
                     DGSMNLVLQKNLYRNALFIVDEASMIAGNQAERDQLFSSRNLLDDLVEYVYSGESCRL
                     LLIGDTAQLPPVGMAISPALDLDYLKSRFNLKIFRHELTEVMRQSLESGILSNATALR
                     QKLAAGETTLPLFAINGMRDILTVPGNELEELFHSAFAPDRISGSVVICRSNKRANLF
                     NKEIRNRILFRENELSAGDHLMVVKNNYYWLDPDSAPGFIANGDIIELQNVRNMEDLY
                     GFRFADVTVRMIDYPLEPAFDVKILLDVLMAESPSLPDLRQKELFGKVLEEVNDIPQK
                     SVRLEKVRSNPYYNALQVKFAYALTCHKTQGGQWENVFIDQGYLKAEQVDAEYLRWLY
                     TALTRATQKVYLLGFMENFFI"
     gene            2997..3389
                     /locus_tag="PKZ74_02065"
     CDS             2997..3389
                     /locus_tag="PKZ74_02065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM91564.1"
                     /translation="MKIYFNGNKQVFADFNEFTVKTDQSARGGGDGLYPEPFALFLAS
                     LGTCAGIFVKSFCDQRGIPTEKIHLTMEPKFDPVRHMIGEFDMTIHVPADFPEKYDQA
                     VIQTASLCTVKRHLRDDIRFNVVVNRLS"
     gene            complement(3424..4755)
                     /gene="mtaB"
                     /locus_tag="PKZ74_02070"
     CDS             complement(3424..4755)
                     /gene="mtaB"
                     /locus_tag="PKZ74_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPM91565.1"
                     /translation="MDNGQWTMTNLPRHRIAFKTLGCRLNQFETDALAAQFRRHGYTI
                     VDFDGDADICIVNTCTVTNQGDAKSKKAISQAIRKQQQPVVVVTGCMVNSQKEMLQTM
                     NGVTYFVENARKTSIFQLVEAHFSGETASPEDFEGDLFAFEPADETFHTRSFIKIQDG
                     CDNFCTYCIVPKVRGRAISRPAADILENIRTVIGFGFKEIVLTGVNIGRYNDHGTDFE
                     NLVEKILELPGDFRLRISSIEPEGFGDKLLELFSHPKMTPHLHLCLQSGSDRILLQMR
                     RFYTLNDFRSLADRIRSRMPDFNLTTDIIVGFPGETDEEFNQTCTVVREIGFSHVHTF
                     KYSTRTGTRAARMPDPVPEKIKQERSLIIRKISDENKRKYRQSMLGKEQCVLVEKYNP
                     KTKLARGYGQHYIPVEFRSEENIHNRFALVMLETLSEQAEPVAAAKKIALL"
     gene            complement(4777..6129)
                     /gene="miaB"
                     /locus_tag="PKZ74_02075"
     CDS             complement(4777..6129)
                     /gene="miaB"
                     /locus_tag="PKZ74_02075"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPM91566.1"
                     /translation="MNYHLLTLGCQMNLSDGERVRTVIDGMGYQWTESEEDAGLLGII
                     ACSVRQKSIDKVYSRINRWNRRKDRQNLVTFISGCILPADREKFLKLFDFVFPMSELN
                     QFPEMLSQYGVVTPASLKAMAEKMRLNESIHEFWHIPAHYNSAFEAFVPIQNGCDKFC
                     AFCAVPYTRGREISRPSAEILDEVKSLIGRNYKSITLLGQNVNSYGIDKKGEEILFPQ
                     LLREIGEIGKASGKEFWVYFTSPHPRDMSDEVIDVIAAYPNLGKQIHLPLQSGDDKVL
                     IRMNRKHSLDKFRSVMQKIRETLPQATVFTDIIVGFSGETEEQFENTVKAMEEFRFNM
                     AYIAQYSPRPGAASSRWADDISPDLKKERFRKLTGQLQKFSLELNREMIGKTFRTLVT
                     GPDRKEGFMSGLTEGKIVLRFPSGDTSLTGNFIDVVIRSATPFSLEGDRVNPSKYNLK
                     "
     gene            complement(6194..6865)
                     /locus_tag="PKZ74_02080"
     CDS             complement(6194..6865)
                     /locus_tag="PKZ74_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPM91567.1"
                     /translation="MDPNITTEYNTLRVRSIRELTPSAYVLRFERNNIPFRAGQHILL
                     GKKGELQAREYSVYSAEQDDHFEVLIKEVEEGLVSKQLKRLAPGDPVNFESPLGYFIL
                     RDEDIRDRKFLFIASGTGISPFHSFVRSYPGLDYKLLHGVRTGSETYEKQDYDSNRLI
                     VCTSRDKNGDFHGRVTDYLRAHPVEDKNTLCYFCGNCEMIHEAYDIMLQQGIPSENLF
                     AEVYF"
     gene            6985..8217
                     /locus_tag="PKZ74_02085"
     CDS             6985..8217
                     /locus_tag="PKZ74_02085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM91568.1"
                     /translation="MMTFEEFGLDEKLIEAITYMGYEKPTPIQEAAIPEILKGRDLIA
                     GAQTGTGKTAAFLLPILNKITRRDVHDVNTLVIVPTRELALQIDQQIHGIGYFLPVTS
                     VSIYGGGGTVDWETEKRSLTGGADIIVATPGKLIAHLNMGYVKFETLEHLVLDEADRM
                     LDIGFYDDIMKIVAYLPKKRQTLMFSATMPPKIKELASKILNDPFIISRELAKPPSGV
                     LQGAYLVFENQKSALINSLIEGKPQCKSILIFTSTKSKVGQIVRALKGRGYKVEGISS
                     DLEQQEREEVLLRFRSRQTRVLVATDVMSRGIDIKDIDLVINYDAPHDAEDYVHRVGR
                     TARAESSGIALTLVNPEDMERFHKIENLIGQLVYKIPLPVELGEGPKWEIKPGGSGRG
                     FRRKGAGPGAKNPGKKRH"
     gene            8420..8791
                     /locus_tag="PKZ74_02090"
     CDS             8420..8791
                     /locus_tag="PKZ74_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91569.1"
                     /translation="MNTQIGNRDKRIALLLVAAFAWLFIGSLVVFHQEHVLGKHFRMN
                     THLFISPKLKDKENYSVALQKSLLKFQETGQMAGIVPGDHAQPGLISYSFSAYKDTYV
                     FRRLHDIVVTNAPLRAPPVTC"
     gene            8898..10175
                     /locus_tag="PKZ74_02095"
     CDS             8898..10175
                     /locus_tag="PKZ74_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91570.1"
                     /translation="MKYFSLITLVSILFLNGFSQGYKAGAFESSLDSVQMGPGYANDV
                     YYSFEEGVVAVVPRTNWDIAFHTTLWSATILSNGASTINGVYLYTYPNADTAGWNTVD
                     TVGMAGWPLLYDSEEYWEEGAFVRNATGHPDYGWGKYNPISHDVVGDSIYILKTSAGQ
                     MKKLWIQRKHSAANTYYIRHANMDGSDDIEQVLQINPYTTKNFVYFSFANNELIDREP
                     DTAAWDIVFTRYMAIQDNGTPYPVVGVCNNLEVYANEFYPVAPDFIDYMSMPFDSTKS
                     PIGWEWKTFDMGTFTWSVADSTAFFVHTRKDNVYKLVFTKFEGSTTGNIVFSKEALSS
                     SAVNENIPDDPVRIYPNPVRDRISVEFNRIIKGKAEIRVFDMTGREVYNTRLTADGKT
                     FSFALPQNADMKGLYILRVSTEEGIYTTRFIAD"
     gene            10179..12407
                     /locus_tag="PKZ74_02100"
     CDS             10179..12407
                     /locus_tag="PKZ74_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91571.1"
                     /translation="MKFIFSYIALFTLTVLLAIPEGYGQHAPVRVVDGKTGQPVPFAH
                     VCFQPLDGTAQTHSITGEDGVVPNAVSSRSVVAVSFVGYETIYDTIDPGMPVTLSLRP
                     KIQEMNEVVVTAQYAPVRADQSIYKVKVISSKQIERKAANNLNDLLSSELNIRISQDG
                     ALGTSMSLQGLSGEHIKFLIDGVPVIGRMNGNIDISQLNLYNVDHIEVIEGPMSVVYG
                     SNALAGVINIITKENKNTRQSAYARGYVESAGVYNFDLGASRKFGDHVVSVTGGRNFF
                     DGFTDNDSLRSMRWKPKRHFFTDGYYLYARPRTKFKLSSSWFDEKLQSRGNLMPPYYE
                     TAFDSYFFTSRWTTRAEVNQKVGEHRYLNLTASYAYYLRQKETFFKDLTTLTETATTN
                     PEDYDTTRFYNSLLRGTFSKSDQKSRFNYQLGIDINLESGKGKRITENHQQIGDYAAF
                     FSIQYKPVKWMELQPGLRYIYNTNYKAPLVYSFNLKFTFLNNYSIRSSYSRGFRAPSL
                     KELYLYFVDVNHNVQGNPELKAENSHNINLSFQYNRETAKSFAGAEINLFYNNINDVI
                     TLAQSAGTLYTYINVDKYITQGFQAAVSYRLYPWATLRVGGGMTGRFNSLAEEQGVEE
                     RFYYSPDAVTTLTYKWLKYDMELTADYKFTGKLPQFYIVGDQVIEGYISAYHTVDLTL
                     QKTFLKNRFTVGAGVKNLFDNTTIPAAGGSGGVHTGGTTSYPVGWGRTYFIQASVNIN
                     KF"
     gene            12409..13431
                     /locus_tag="PKZ74_02105"
     CDS             12409..13431
                     /locus_tag="PKZ74_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HPM91572.1"
                     /translation="MKSLNISLLMLFLFLITSCFKEDEKILPHDPGDVNTITIEMTNN
                     YRYQSWFDLGTGEVVRTGLKKSWDLSFDASPQGWLILLNSSNFMLAARSGMADIAVPL
                     DTAGLDWRFDASSGNPDSLSFSGWVNFSEGDSVKIYSNEVFVIDRGYDEAGNLRGMRK
                     VVFEEVSDSIYRFRFAHLDGTGVTEMVIRKDSEVNYVCFTFDEGGRQIDIEPESQSWD
                     LLFSQYTTLLYTNEGDPYPYLLTGVLSNPAGVEVAQDTVFNFGDIDFDVAQGLLYSKA
                     LDEIGYDWKDVVGDVSSGSVTYVIIEGLNYIVRDPEGYYYKLRFTGFYNNSGEKGYPT
                     FEYQRL"
     gene            complement(13463..14215)
                     /locus_tag="PKZ74_02110"
     CDS             complement(13463..14215)
                     /locus_tag="PKZ74_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM91573.1"
                     /translation="MLKVVIVDDEPDAVKFIQGIIEEYCPNLLVAGVAHSARDGVAVI
                     QQNQPDLVFLDVQMPHGSGFDLLSGFPGKTFDVIFITAFNHYAIQAIKFSAVDYILKP
                     VNINELIEAVKKVEEKRASREYRNLDFTHLLENLSVPFPSKLAIPTSDGIEYLNTSEI
                     VRIEADRSYSWFYLTEKRKYLVSRNLKEYQELLQDLNFFRPHNSHLINMNFVKKFIRH
                     EGGYIEMTDGSNVPISRGKKDLFLLQMAKISK"
     gene            complement(14229..16124)
                     /locus_tag="PKZ74_02115"
     CDS             complement(14229..16124)
                     /locus_tag="PKZ74_02115"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM91574.1"
                     /translation="MTDGFGQTRNPEKKTDSLVNVLNTMATGDTARIPVLKSLSKILL
                     SKDARQTLDYCDELIGISETNHLPVDPMVFLTKARAYSRIPDNEKALANFKTAMTLAM
                     EKKDTASIAWIHNGTGYHYYALRNVPSALFEFNKAIELGIILKDTMLLSDAWFGKVVT
                     YNIFEELEGLQNSINQYLLYADSVTEKRRLSDAYRILGTYWRGRNEFQEAIDANWKSF
                     TIASQIKDSILMGAALNHMAWYYYEMGNLEKSLQLYRQNIGFYHSEKSNALANVYGNI
                     GNIYRDWERYPEAISNYNKSIELARKNRDIYNLSWLYEDISVLYHRIGDYRLAYENQL
                     LASAYGDSLATMTYQQNLASARAQYEAEKTAKELELLSVKLQRNRLFTWVLIGGFFLV
                     TSVTFLLINRYRYKSRQRIDAMNHTISELNQTNLRQQMNPHFIFNTLNSIQYYVFQND
                     KISSNNYMTKFASLIRKTLENSRHTEISIKEELDSLRLYLELEELRFKEKFEWSIRVD
                     EEIDTLAYKIPTMLIQPYVENAITHGLMNKENGKGALTVELQLLPDHICCIVEDNGIG
                     RARALEIRQQKNENHRSLGTNITESRLKLVNEIYGKNMQVVYTDLMDESGEPAGTKVE
                     INIPIIT"
     gene            16372..17856
                     /locus_tag="PKZ74_02120"
     CDS             16372..17856
                     /locus_tag="PKZ74_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPM91575.1"
                     /translation="MKILLLFLVLTGMCACMAPEPPKAEPAGDEKNVADEVRVINQGN
                     VAYDNYFTEKTMRIDYFHTGTAVSEVFAPDRIVSDGSWPGSKTILIDPLRFGLYYFEI
                     TDKETDILLYSRGFASIFGEWQTIPEAGEGYATFHESIRFPWPKKPVNIILKKRDTIN
                     VFRQIWSTSVDPASRKVNPADLVHDEKVDVILDNGPAPVKLDIVILGDGYAMNEMEKF
                     RMDSKRMAEVLLSREPFLNRRADINIRAVETPSPVSGVLRPHPGVFRRTPLSAHYGSF
                     DSERYVLAYDNRTIRDVASAVPYDFMVILVNERNYGGGGIYNLYTTVSVDNKFGEYIM
                     VHEMGHHMGALADEYYTSSIAYEIPVVKTEPWEPNITALFDKDNLKWKDLVDPGTPIP
                     TPWNKEPFDRFGYRIQQERDSLRAAKVPEELMEALFMRQMKQEDEFFAAEKYREQVGA
                     FEGAGYLAKGLYRPQIDCIMYTRHMEFCKVCRRSLEDVINMYSR"
     gene            17862..18728
                     /locus_tag="PKZ74_02125"
     CDS             17862..18728
                     /locus_tag="PKZ74_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM91576.1"
                     /translation="MKLTGFIFFRLFSGFIGLLPFPVLYSFSFFLSFILKRVIKYRKR
                     IILQNLTGCFPRMDERALNDTMGRFYLNLTDVMLEGLKAFTMSGRQVLARYRIRNAEV
                     IKPYVDAGRSVICVTGHYGNWEWGALSPPLQTRFRFIAFYKPLKYKRFDSYARNNRSR
                     FGTYLAPIRETTKTFEENRGNPAVYLMAADQNPSNREMAYWVDFLNRETAFLHGPEKH
                     ARINNYPVVFAAIRRTARGYYELELSVLADDPSVLPEGEITTRYAASMEKLIREDPAN
                     WLWSHNRWKYSR"
     gene            complement(18720..>19135)
                     /locus_tag="PKZ74_02130"
     CDS             complement(18720..>19135)
                     /locus_tag="PKZ74_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPM91577.1"
                     /translation="KDMFKTSVGKYVSPQKLELLLGQEKLIEQVIAVGDNRKFVSALI
                     VPSFDNLKTTAENLGIDINDRHKVVSHDTVQKIFRDKLEELQQELTPYERVVKFTLLT
                     EPFSIENNAMTSTLKLKRKVITEKHIAVIEEMYSS"
BASE COUNT         4746 a         4389 c         4825 g         5175 t
ORIGIN      
        1 gcgatgtaac ctggggaatc aatttatcca atatcggaag taagatttca tacagtaaac
       61 aatccaccaa gaaggatttt attccaacga accttcgcct gggaggagga ttgaaaatga
      121 attttgacga ttataattcg ctggccttgc tgcttgatct caacaagctg atggttccca
      181 cgccccctat ttacctgagg gattcgtcag ggaacccggt cattgatccg gctaccaata
      241 atcctgtcat tgataaaggg aaagatccga atgtgtcgcc cgttgccggg attatccagt
      301 cgtggtatga tgcacctcac ggattcagtg aagagatgag agaatgggca atagctttcg
      361 gaatggaata ctggtatgcc aaacagttct cattgcgtac gggattcttt tatgaagaca
      421 agaccaaggg caaccggaaa tttatcacgg tcggtgcagg cttgcgctac agtgttttcg
      481 gtctggatct gtcgtacctg atcccctttg aacagcagca tccattggaa aacacgctcc
      541 ggttcaccct ccatttcaac tttgatccgg ataaagagag cgatctcccc aggaaataaa
      601 aaccgggaac aattaattca ggcgggtcag attgaggttt gccatttcga ggcggatcgt
      661 gccgttttca ttaacggtga atttaacgga aaccacatcg ccttcattca gttcaatcaa
      721 tcctgagaac gaaaaattct gagctttgct gccttctttg aaccagcagc gggtgcgcat
      781 gtcatcctgc cggataccat taacgaagag cgctgcttca gtgatcttgc caccgctcga
      841 catctggagc gccgcattga atgagacggt atatgtgcct gggtttaaaa tcgttaaagt
      901 ggaagcagaa gcggccattt cactggtgtc tccttcctga gccgtattcc agggcatcca
      961 ggttccgcct gaagcgacct gaattgttgt gaaatccccg atattatggt actcgtataa
     1021 ttcgccaaaa acggctgccg gctgcggagc atttgccaaa ttcattacct gttcaacggt
     1081 ggccagggcc gaccatgtca taccgttcca gatataaaag cctgcatcga ggttatttcc
     1141 tcccaggtta taaatcatca atccggttgc cggattttgg atcggagcat tgttattgac
     1201 tttacttaag agggtgaccc tggggatcag gagccctttg tcaggagcgt taatatcgag
     1261 gactgcgctg ggatcgggat cggcacccga tgtattgatc gcaacctgcg aaaagatgtt
     1321 ctgcgaaaaa aacaataaaa taaccaaccg gcttagtatc actaagtttt tcatgacctt
     1381 gggggttttg ggtttagcag atggttcggt ctaaaaaagc ggtgcaaagg tagtatttaa
     1441 tttattgctt ttacatttat atgaaaaaat tttccataaa cccgagaaga tatacttttt
     1501 gagtggccct ggtaagggct gtataaagcc aacggaggta ttcggcatcc acttgttccg
     1561 ctttcagata cccctggtcg ataaagacat tttcccactg tccgccctgc gttttgtggc
     1621 aggtgagggc gtaggcgaat ttaacctgta gcgcgttgta ataaggattt gacctgacct
     1681 tttccagcct gaccgatttt tgcgggatat cattgacctc ttcgaggact ttgccaaaga
     1741 gttctttttg tcttaaatcg ggaagagatg gtgattcagc catcaggaca tccagcagga
     1801 ttttcacatc aaacgccggt tctaagggat aatcaatcat tctgacggta acatcggcaa
     1861 accggaaacc atacaggtct tccatattcc tgacattctg gagttcgatg atatcaccgt
     1921 tggcaataaa accgggtgcg gaatctgggt caagccagta gtaattgttt ttgacgacca
     1981 tcaggtgatc tccggccgat aattcatttt ccctgaagag gatccggttc cggatttctt
     2041 tgttgaacag gtttgccctt ttattcgaac ggcagatcac gaccgatccg gagattcggt
     2101 caggggcgaa ggcgctgtga aagagctctt ccagttcatt gcccggaact gtgaggatgt
     2161 cgcgcattcc attgatggca aacagcggga gtgtggtttc accggcggca agtttctgcc
     2221 tgagcgctgt tgcattcgac aaaatgcccg attcgagcga ttgccgcatg acttccgtca
     2281 gttcatggcg aaagatcttc aggttaaacc tcgatttcag gtaatccagg tcgagcgccg
     2341 ggctgatagc catcccgacg ggaggcaact gggctgtgtc gcctatcagc agcagccggc
     2401 aggattcacc ggagtaaaca tattctacga ggtcatccag caggttccgt gatgaaaaca
     2461 gttgatcgcg ttcagcctga ttaccggcga tcatggaggc ttcatcaaca ataaacaggg
     2521 cattccggta caggttcttc tgcaatacca ggttcatgct cccgtcgcgc ccgacacgct
     2581 ggaaatagat ccgtttgtga atggtccacg cgggctggcc ggcatagcca gagagaacct
     2641 tggcagccct tccggtcggg gccaggagga cagctttttt gcggataacc ggaaggattc
     2701 gtaccattgc gctgacaaaa ctggttttcc cggttccggc atagcctttc agaataaaaa
     2761 ccgaccaggg ctcaggatcg ccgataaatt tcgccagttc atcggccagg ttttgctggc
     2821 cccgcgtagg ctcaaatgga aaattgcgga atatttctcc ctggatagtc attgtggccc
     2881 aaaattaaca aatcccccgg atcggggtgc cataatgtca tcaccgggac acgaatattc
     2941 cgctggcctg gaaattgctg acaaccgcat cttttaactt aaatatcgac atacttatga
     3001 agatttattt taacggaaac aaacaggttt tcgccgattt taatgagttt acagtaaaaa
     3061 ccgaccagtc agcccgcgga ggaggcgacg gcctttaccc ggagcctttt gcccttttcc
     3121 tggcttctct tggaacctgc gcaggtatct ttgttaaatc tttctgcgac cagaggggaa
     3181 tacccaccga aaagatacac ttgacgatgg agcccaaatt cgatcctgtc cgtcatatga
     3241 tcggagagtt tgacatgacc atccatgttc cggctgattt tcctgagaaa tatgaccagg
     3301 cggtcatcca gacggccagc ctttgcacgg tgaaacgcca cctgcgtgat gatatccggt
     3361 tcaatgttgt tgtgaatcga ttgtcatgaa tccgatagag ttgcatgaga ggattgccgg
     3421 ctctcaaagg agggcaatct tttttgcggc agccaccggt tcggcctgtt ctgacagggt
     3481 ttcgagcatg acaagagcaa aacggttgtg gatattttct tcagagcgga attcgacagg
     3541 aatgtaatgc tggccatatc ccctggccag ctttgttttc gggttgtatt tttccaccag
     3601 cacacattgc tcttttccca gcatcgattg ccggtattta cgcttatttt cgtctgaaat
     3661 cttccgtatg atgaggctcc gttcctgctt tattttttcg gggaccgggt cgggcatacg
     3721 ggcggcgcgg gtgccggtac gggtgctgta tttgaatgta tgcacatgcg agaagccaat
     3781 ttctcttaca accgtgcagg tttggttgaa ttcttcatcg gtttcgccgg gaaagcctac
     3841 aataatatcg gttgtcaggt tgaaatcggg catccgggac ctgatcctgt cggccaggga
     3901 tcgaaaatca tttaacgtat aaaagcgccg catttgcaac aggatcctgt cggaaccgct
     3961 ctgaaggcaa agatgaaggt gcggggtcat tttcggatgg ctgaataatt ccaggagttt
     4021 atcgccgaat ccttccggtt cgatggaaga aattcgcagc cggaaatcac cgggcagttc
     4081 cagaatcttt tccaccaggt tctcgaaatc ggtaccatgg tcattgtatc ggccgatgtt
     4141 gacgccggtc agtacgatct ctttgaagcc gaaaccgatg accgtacgga tattttccag
     4201 gatgtcggct gccggcctgc tgatagcccg gcccctgacc tttggaacaa tgcaatacgt
     4261 acagaagtta tcacagccat cctggatttt gataaagcta cgggtatgaa aggtctcatc
     4321 ggcaggttca aaggcgaaca aatctccttc aaagtcttcg gggcttgccg tttcgccgct
     4381 gaaatgggct tcgaccagct ggaatattga ggtcttcctg gcattttcca caaagtaggt
     4441 caccccgttc atagtctgca gcatttcttt ttggctgttg accatacatc cggtaacgac
     4501 gacgaccggt tgctgctgtt tcctgattgc ctggctgatg gctttctttg acttggcatc
     4561 accctggttg gtgacggtgc aggtgttgac gatacagata tcggcatccc cgtcaaaatc
     4621 cacgatggtg taaccatggc ggcgaaactg ggcggccagt gcgtcggtct cgaactggtt
     4681 cagtctgcaa cccagggttt taaaggcgat acggtggcgg gggaggttag tcattgtcca
     4741 ttgtccattg tccattttag tggattattg gttattttat ttgagattgt atttagaagg
     4801 attgacccga tctccttcta atgaaaaagg tgtggcggag cggatcacga cgtcgatgaa
     4861 gtttccggta agggaggtgt cgccggaagg aaatctgagg acgatttttc cttctgtcag
     4921 gccggacata aaaccttctt tccggtcggg gccggtaacc agggttcgga atgttttccc
     4981 gatcatttcc cggttcagtt ccagggagaa tttctgcaac tggccggtca gtttcctgaa
     5041 gcgttctttt ttcagatcag gcgaaatatc gtcggcccac cgggagctgg cggctcccgg
     5101 acgcggggag tactgggcga tatacgccat attgaaccgg aattcctcca tggccttcac
     5161 cgtattttcg aattgctctt ccgtttcacc ggagaaaccg acgataatat cggtgaatac
     5221 ggttgcctgc ggcagggttt cgcgtatttt ttgcattacc gacctgaatt tatcgagcga
     5281 atgtttgcgg ttcatccgga tgagtacttt gtcgtcgccc gattgcaggg ggaggtgaat
     5341 ctgtttcccc aggttcggat aagcggcaat gacgtcgatc acttcatccg acatatccct
     5401 gggatgaggc gatgtaaagt acacccagaa ttcctttccg gaggcctttc cgatttcacc
     5461 gatttcacgc aataattgcg ggaataatat ttcttctcct ttcttgtcga tgccatagga
     5521 attgacattc tgccccagca gggtgatgga tttataattc ctgccgatca ggcttttgac
     5581 ctcatcgagt atttcggccg atggccttga aatttcgcgg cctctcgtgt atgggacagc
     5641 acagaaagcg cagaatttgt cgcatccgtt ctgaatggga acgaaagcct cgaaggcgga
     5701 gttgtaatga gcagggatat gccagaattc atgtatgctt tcattgagcc tcattttttc
     5761 ggccatggct ttcagcgagg cgggggtgac cactccgtac tggctgagca tttccgggaa
     5821 ctggttgagt tcgctcatag ggaatacgaa atcaaacagt ttcaggaact tctccctgtc
     5881 ggccggcagg atacatccgg agatgaaagt aaccaggttt tgacggtctt tcctccggtt
     5941 ccatcgattg atccgggaat agactttatc gatcgatttt tgccttactg aacaggctat
     6001 gatgcccagc agacccgcat cttcttccga ttcggtccac tggtatccca tcccgtcgat
     6061 gacggttcgc accctttccc cgtcggagag gttcatctgg cagccgagag ttagtaaatg
     6121 atagttcaaa gttcagcgtt caaagttatg cgtgagagtt acgggtatta ttttttaata
     6181 gctgatggga ttcctaaaaa tatacttctg cgaacaggtt ttcggaaggg atgccctgct
     6241 gcagcatgat gtcataggct tcgtgtatca tttcgcagtt tccgcaaaaa tagcacaacg
     6301 tatttttatc ttctacgggg tgagccctca ggtagtcggt cacccggcca tggaaatcgc
     6361 cgttcttatc ccgggaggtg caaacaatca gccggttgct atcataatcc tgtttttcat
     6421 aagtttcgct cccagtcctt accccgtgaa gcaatttgta atcaagtccc ggatacgatc
     6481 tgacgaaact gtggaaaggg gagatgccgg taccgctggc tataaataag aatttccggt
     6541 cgcggatgtc ttcatctctc aggataaaat aacccaaagg actttcaaag ttgaccggat
     6601 cgcccggggc caatcttttt aattgttttg aaacaaggcc ctcttccact tccttgatca
     6661 ggacttcgaa atgatcatcc tgctcggcgc tgtaaacact gtattcacgg gcctgaagtt
     6721 ctcctttttt tccgaggagg atatgctggc cggcccggaa agggatattg ttccgttcga
     6781 accggaggac ataggcagaa ggtgtaagct cacggatact cctgacccgg agcgtgttat
     6841 attctgtagt tatatttgga tccactttat ttatcgctta ttttaattta atcttaataa
     6901 tttgcaaaga aacaaatttt ccggtttatg ccgttttcgt cgagggtcgg attaatcctt
     6961 acctttgccg gaaaatgaat attgatgatg acttttgaag aattcggcct ggatgagaaa
     7021 ctgattgagg ctattacgta catgggttac gagaagccga cgcccatcca ggaggctgcc
     7081 attcccgaaa tcctgaaagg gcgtgacctt atagccggcg ctcagacggg gacgggaaag
     7141 acggccgcat ttcttcttcc catccttaac aaaattaccc gccgggatgt tcacgatgtg
     7201 aatacattgg tgattgttcc tacacgtgaa ctggcgttac agatcgatca gcagatccat
     7261 ggcatcgggt attttctgcc cgtcacttcc gtttcgatct acggaggggg cgggacggtc
     7321 gactgggaga cggagaaacg ctctttgacc ggcggcgccg acattatcgt ggccaccccg
     7381 ggcaagctga ttgctcacct gaatatgggc tacgtaaaat tcgagacgct ggaacatctt
     7441 gtgctcgacg aagccgacag gatgctggat atcgggtttt atgacgatat catgaaaatc
     7501 gttgcctacc ttccgaagaa aaggcagacg ctgatgttca gcgccactat gcctcccaag
     7561 ataaaagaac ttgcctccaa aatcctgaac gatccattca ttatttcgcg tgaactggcc
     7621 aaaccgccat caggcgtatt acagggggcc tacctggtat tcgaaaacca gaaatcggcg
     7681 ctcattaaca gcctgatcga aggcaaaccg caatgcaaaa gcatcctcat ctttacctct
     7741 accaagagca aggtagggca gatcgtcagg gccctgaagg gcagaggata caaagtggaa
     7801 gggatttctt cggatctgga gcagcaggag agggaagagg tgttgctccg cttccggagc
     7861 cgccagaccc gcgtgcttgt agccactgat gtgatgagcc gcgggatcga tatcaaggat
     7921 atcgacctgg tgattaatta tgatgcgccg catgatgccg aagattatgt gcaccgcgta
     7981 ggccggaccg cccgggccga gtcttcagga atcgcattga cactggttaa tccggaggac
     8041 atggaacggt ttcataagat tgagaatttg atcggccaac tggtttataa aattccattg
     8101 ccggttgagt tgggtgaagg ccctaaatgg gagataaagc ccggcgggag cggcaggggt
     8161 tttcgccgga aaggcgccgg gccgggagct aaaaacccgg gaaagaagag gcactaaacc
     8221 cgtgatgttg ccactcaatt ttgttgaaac tgctcttgag gattacgatg aaaatgctat
     8281 catcttatct gaattcttta taaattatac ttataaatat ttgaataaaa gataattata
     8341 aattctctat catttattca aacattcaca taatttcata tcgaattgct tattattttt
     8401 gcaccgaatt ttaattgata tgaatacaca gatcgggaac agggataaaa ggattgcgct
     8461 gctgctggtt gctgcatttg cgtggctttt tatcggttcc ctggtcgttt tccaccagga
     8521 gcatgtcctg ggaaaacatt tcaggatgaa cacccatttg ttcatctctc cgaagctgaa
     8581 agacaaggaa aattactccg tcgcgcttca gaaatcattg ttaaagttcc aggaaaccgg
     8641 ccagatggcc ggtatcgtac ccggagatca tgcccaaccg ggactcatat cctattcatt
     8701 ttcagcatac aaggatactt atgttttcag gcgtttgcat gatattgtcg tgacgaacgc
     8761 cccgcttagg gcgcccccgg ttacctgcta atcaggatgt ggtctgtcgg gccacctctt
     8821 ttccttcatt ttctttcatt catttttcct ctgtaattta ttagcattca atgtttcaca
     8881 atctaatcat atcacgcatg aaatattttt cactaatcac tttagtatcg attttatttt
     8941 tgaacggatt cagccagggt tacaaagcag gcgcttttga gagcagcctg gattcggttc
     9001 agatggggcc cggttatgcc aatgatgttt attacagttt tgaagagggc gttgtagccg
     9061 ttgtgccccg aacgaactgg gacattgcct ttcacacgac gctctggtcg gccacgattc
     9121 tgagcaacgg cgcttccacc atcaatggag tatatcttta tacctatccg aatgccgata
     9181 cggcgggatg gaacacggtg gatacagtcg gaatggccgg atggcccctt ttgtatgatt
     9241 cagaagaata ctgggaagaa ggcgcttttg tacgcaatgc aaccggccat cccgattatg
     9301 gatggggaaa atacaatccc atatcgcatg atgtggtcgg ggactccatt tacatcctga
     9361 aaaccagtgc cggacaaatg aagaaattat ggatccagcg caaacattcg gctgccaata
     9421 cctattacat ccgccatgcc aatatggatg gcagcgacga tatcgagcag gtgctgcaga
     9481 tcaatccgta tacgacgaaa aactttgtct atttttcctt tgccaataat gaactgattg
     9541 acagggagcc tgacacggca gcatgggaca tcgtgtttac acgttacatg gcgatccagg
     9601 ataatggaac cccatatcct gtagtaggcg tatgcaataa cctggaagtt tatgcgaatg
     9661 aattttaccc ggtcgcgcct gatttcatcg attatatgag tatgccgttc gattccacca
     9721 agtcgccgat cggctgggaa tggaaaacat tcgatatggg gacatttaca tggagtgtcg
     9781 ccgattccac tgctttcttt gtccatacgc gcaaggataa tgtttataaa ctggtattca
     9841 caaaatttga aggcagtacc acggggaata tcgtattcag caaagaggcc ctttcatctt
     9901 ccgccgtaaa cgaaaatatt ccggacgatc cggtccggat ttacccgaat cctgtcaggg
     9961 acaggatcag cgttgaattc aacagaatta tcaaaggaaa ggcagagatc cgggtattcg
    10021 acatgacggg gcgcgaggtt tataacaccc ggctgacagc cgatggcaaa accttttcgt
    10081 ttgcattgcc tcaaaatgca gatatgaagg gtttatacat cctcagggtg agtacggaag
    10141 aaggcattta taccacccgt tttattgccg attaagcgtt gaagtttatt ttttcctata
    10201 tagcgttgtt tacattgacc gtgctgctgg ccattccgga aggttatggc cagcatgctc
    10261 cggtcagggt tgttgatggt aaaacgggcc agccggttcc gtttgcccat gtttgttttc
    10321 agccactcga cggaacggcg caaacccatt ccattacagg cgaagacggg gttgtcccca
    10381 atgcggtatc atccaggtcg gtggtggccg tatcttttgt gggttatgaa acgatttacg
    10441 ataccattga tcccgggatg ccggtcactt tatcgttacg gccgaaaatt caggaaatga
    10501 atgaggtggt cgtaacggcc cagtatgccc cggttcgcgc cgatcagtcc atttacaagg
    10561 tgaaagtgat cagcagcaag caaattgagc ggaaggccgc caataatctg aatgacctgc
    10621 tgagcagcga attgaatatc agaatctcgc aggatggcgc cctgggtacg agcatgagcc
    10681 tgcagggcct ttccggggag catattaagt ttttgatcga tggagtacct gtcatcggcc
    10741 ggatgaacgg caatatcgat atcagccagt taaatcttta caatgttgat catatcgagg
    10801 tgattgaagg gccgatgtcg gtggtatacg gatcaaatgc actggccggg gttatcaata
    10861 tcatcaccaa agaaaataaa aatacccggc agagcgctta tgccagggga tacgttgaat
    10921 cggccggggt gtataatttt gacctgggcg cctcccgtaa attcggcgac catgtggttt
    10981 ctgtaacagg cggaagaaat tttttcgacg gtttcaccga taatgattct ctccgatcga
    11041 tgcgctggaa gcccaaaaga cattttttta ccgatggata ttacctgtat gcccggccga
    11101 gaaccaaatt caaactgtcg tcatcgtggt tcgacgaaaa gctgcagagc agggggaacc
    11161 tgatgccccc atactatgaa acggctttcg acagctattt ctttacatcg agatggacga
    11221 cccgggccga ggtcaatcaa aaggtcggcg aacaccgcta tctgaacctg acggcttcct
    11281 acgcctatta cctgaggcag aaagaaactt tcttcaagga tttgaccacg ctgacagaga
    11341 cagcgaccac gaatccggaa gattacgata caacccgctt ttataacagc ctgttaaggg
    11401 ggactttcag caagagtgac caaaaaagcc gtttcaatta ccagctgggt attgacatca
    11461 atcttgaatc gggcaagggc aaaaggatca cggaaaacca tcagcagatc ggcgactatg
    11521 cggccttttt cagcattcag tacaaaccgg tgaagtggat ggaacttcag ccgggtttgc
    11581 ggtacatata caataccaat tacaaggccc cgctggtcta ctcatttaat ttgaaattta
    11641 ctttcctgaa caattacagc atccggtcct catacagccg gggtttcagg gcgccttcct
    11701 taaaggaact gtatctttat tttgtggatg ttaatcataa tgtacagggc aatcctgaac
    11761 tgaaagcgga aaattcgcac aacatcaatc tttcattcca gtataaccgt gagacggcca
    11821 aatcgtttgc cggagcggag atcaatcttt tttacaacaa tatcaatgat gtaattacgc
    11881 tggcccaatc cgccggaaca ttgtacactt atatcaatgt cgacaagtac atcacccagg
    11941 gtttccaggc agccgtcagt taccggttgt atccttgggc aaccttgcgg gtgggcggcg
    12001 gtatgaccgg ccggttcaat tcactggcgg aagagcaggg tgtggaagaa aggttttatt
    12061 acagccccga tgccgttaca accttgacct acaagtggct gaaatatgat atggagttga
    12121 cggcagatta caagttcaca gggaaactcc cccagtttta cattgttggt gaccaggtaa
    12181 ttgaaggata tatcagcgct taccacacgg tggacctgac gcttcagaaa actttcttaa
    12241 agaaccggtt cacggttggc gccggtgtca aaaatttatt cgataacacg actattccag
    12301 ctgcgggcgg atcgggcgga gttcatacag gagggacgac ctcatatcct gtcggctggg
    12361 ggcgaacgta ttttatccag gcatcggtga atatcaacaa attttaaaat gaagtcatta
    12421 aatatcagct tgttgatgtt gtttcttttc cttataactt cttgttttaa ggaagatgag
    12481 aagatattgc cacacgatcc gggggatgtg aataccataa ccattgagat gactaacaac
    12541 taccgctatc agtcgtggtt tgacctgggt accggggagg tggtccggac tggtttgaaa
    12601 aagagctggg acctttcgtt cgatgcttct ccccaagggt ggcttatcct gttgaattcg
    12661 tcgaacttta tgcttgcagc ccgtagcggc atggctgata tagcggtacc gctcgatacg
    12721 gccggcctgg actggagatt cgatgcttct tcgggcaatc ccgactccct ctcattttcc
    12781 ggctgggtta atttcagtga aggcgattcc gtaaaaattt actccaatga ggtatttgtc
    12841 attgacaggg ggtacgacga ggccgggaac ctccgcggga tgagaaaagt tgtttttgag
    12901 gaggtcagcg acagcattta ccggttccgg tttgcccatc tcgatggcac cggtgtgaca
    12961 gagatggtga tccggaaaga cagtgaagtg aattatgtct gttttacttt tgatgaaggg
    13021 ggcaggcaaa tcgacattga accggagagc caaagctggg acctgctgtt ttcgcaatac
    13081 acaacgctgt tgtatacgaa cgaaggggat ccctatccat atttattgac aggtgttttg
    13141 tcgaatcctg ccggtgtgga agtggctcag gatacggttt ttaatttcgg agacatagat
    13201 tttgatgtgg cccaggggtt actctattcg aaagcgctgg atgaaatcgg atacgactgg
    13261 aaagatgttg ttggggacgt aagcagcgga agtgtgacct atgtgatcat cgaagggctc
    13321 aattatattg ttcgcgatcc cgaaggctat tactataaac ttcgttttac gggattttac
    13381 aataacagcg gtgaaaaggg ttatccgacc tttgagtacc agcggcttta atggttcggg
    13441 gttccaggtt ccgggttccc ggttatttcg agatttttgc catctgcaat aagaacaaat
    13501 cttttttccc ccgtgagatg gggacgtttg agccgtcggt catttcgata tacccgccct
    13561 cgtgccggat aaactttttt acaaaattca tattgatgag gtgggaatta tggggccgga
    13621 agaagttaag gtcctgcaaa agttcctggt attctttgag atttctggaa acaaggtatt
    13681 ttcttttttc ggtcaggtaa aaccagctgt aactccggtc ggcttcaatc cgaacgattt
    13741 cggaggtatt caggtactcg ataccgtcgg aagtcggaat agccagtttg ctggggaaag
    13801 gcacggatag gttctccagc aggtgggtga aatcgaggtt tcggtattcg cggctggctc
    13861 ttttctcttc gactttttta acggcttcta tcagttcatt gatattcacg ggcttgagga
    13921 tgtaatccac cgcgctgaat ttgatggcct ggattgcgta atggttaaat gccgtaatga
    13981 agatcacatc gaaagttttt ccgggaaaac cggaaagcag gtcaaaaccg ctgccatggg
    14041 gcatttgcac atcgaggaag accaggtcgg gctggttctg ctggatcact gcgacgccat
    14101 cgcgggccga atgagctact cctgcaacca gcaggttcgg gcaatattct tcgatgatcc
    14161 cctggataaa tttcaccgca tccggttcat cgtcaacaat gacaacttta agcatagtat
    14221 gttttatttc aggttattat aggaatattg atttcgactt ttgtcccggc gggctcaccc
    14281 gattcatcca tcaggtcggt ataaacaacc tgcatatttt tgccgtaaat ttcattgacc
    14341 agtttcagcc tggattcggt aatgttcgtg cccaggctgc gatggttctc gtttttctgc
    14401 tggcggattt ccaaagcccg ggccctgccg atgccgttgt cttccacaat gcagcagata
    14461 tggtcgggca ggagctgtaa ttcgacggtt aatgctcctt ttccgttttc cttattcatc
    14521 agcccatgcg taatagcatt ttcaacatat ggctggatca gcatggtcgg aattttatag
    14581 gccagcgtat cgatctcctc atcgacccgg atcgaccact cgaatttctc tttgaaccga
    14641 agttcttcca gttccaggta aagccttaac gaatccagtt cctctttgat ggatatttcg
    14701 gtgtgccggc tgttttccaa tgtcttacgg atcagggaag caaacttggt catgtaatta
    14761 ttggaagaga tcttatcatt ctggaataca taatactgaa tcgaattcag cgtattaaag
    14821 atgaaatgcg gattcatctg ctgccggagg ttggtctggt tcagctcgga gatggtgtgg
    14881 ttcatggcgt cgatacgctg ccgggatttg taccggtaac ggttgatcag aaggaaggtg
    14941 acagaggtga ccaggaagaa ccctccgatg agtacccagg taaacaaccg gttgcgttgc
    15001 agttttaccg aaagcaattc gagttctttg gccgtctttt ccgcttcata ctgggccctg
    15061 gcgctggcca ggttttgctg gtaagtcatg gtggccaggg agtcgccgta agccgaagcc
    15121 aggagctgat tttcataagc caggcggtag tcgccgatcc ggtggtacaa cacgctgata
    15181 tcctcataga gccacgacag gttataaata tcccggttct tcctggcaag ctcaatgctt
    15241 ttgttatagt tggagatggc ttcgggatac cgctcccagt cacggtatat atttccgata
    15301 ttgccataga catttgccag ggcattgctt ttctccgaat ggtaaaaacc gatattctgc
    15361 cggtatagct gaagtgactt ttccaggtta cccatctcat aatagtacca tgccatatgg
    15421 ttcagggctg cccccatcag gatggagtct tttatctgcg atgcaatcgt aaaggatttc
    15481 caattggcgt cgatggcttc ctgaaactcg ttccttcccc gccagtaggt tccgagtatc
    15541 cggtaagcgt cggacagacg ccgtttctcc gtaaccgaat cggcataaag caggtattga
    15601 ttgatcgagt tttgcaagcc ttccagctct tcaaaaatat tatatgtcac caccttgccg
    15661 aaccatgcgt cggatagcag cattgtgtct ttgagtatga ttccgagttc gatggctttg
    15721 ttgaattcga agagcgccga aggaacattg cgcagtgcat aatagtgata accggtacca
    15781 ttgtgaatcc aggcaattga cgccgtgtct ttcttttcca tggccagtgt catggcggtc
    15841 ttaaagttcg ccagcgcttt ttcattatcc ggtatacggc tgtatgcccg ggctttggtc
    15901 aggaaaacca ttggatcgac cggaagatgg ttggtttcgg aaatcccgat caattcgtcg
    15961 caatagtcaa gggtttgccg tgcatctttg ctcaataaaa tttttgacag cgattttaat
    16021 accggtatcc ttgctgtatc cccggttgcc atggtattca gcacattaac caggctgtcg
    16081 gttttctttt cagggttcct ggtttggcca aaaccgtctg tcattaatag taatattatt
    16141 aatgcactga taatgaatcg tatattttct ccattatgga acataatacc agtaagccgg
    16201 ttaatttttt tggttaagta gatgcaaaga tatgatggaa agttgaagat tcgggcaaga
    16261 gttttccggt accggatgcg aaccgatgat ttgccggatt ggaacaacag attggataaa
    16321 ctgtcggagt tattggttaa taattaattt tactgcgtta aaaaccggat tatgaaaatt
    16381 cttttgttat ttcttgtatt gacaggcatg tgtgcctgca tggcgccgga accgccgaaa
    16441 gcggaacctg ccggagatga aaaaaatgtc gctgatgaag tgcgtgtgat caatcagggc
    16501 aatgtggcct atgacaatta ctttacggaa aaaacgatgc gtattgatta tttccacacc
    16561 ggtacagcgg tatcggaggt ctttgcgccc gacaggattg tatccgatgg ttcctggccg
    16621 ggaagcaaaa caatcctgat cgacccgctg cggtttggtt tatattattt tgaaatcacc
    16681 gacaaggaga cagatatcct cctgtactcc aggggctttg ccagcatttt cggagaatgg
    16741 cagacgattc ccgaagccgg cgaagggtat gcgactttcc atgaatcgat ccggttcccc
    16801 tggccgaaaa agccggtgaa tatcatcttg aagaaaaggg ataccataaa tgttttccgc
    16861 cagatatggt cgacctccgt tgatcccgca tcccgaaagg taaacccggc cgatctggtt
    16921 catgatgaaa aagtcgatgt tatcctggat aacgggccag ctcccgtaaa attggatatt
    16981 gtcatccttg gcgatggtta tgcaatgaat gagatggaga aattcaggat ggattcgaag
    17041 cgaatggcag aagtcttact cagccgggaa ccatttttaa accggagagc cgacatcaat
    17101 atccgggcgg ttgagactcc atctcccgtt tccggcgtcc tgcggccaca cccgggagtt
    17161 ttcaggcgaa cgccactgtc ggcccattac ggcagttttg actcggagcg gtatgtcctg
    17221 gcctatgata accggaccat ccgggatgtg gcatccgctg tgccctacga ttttatggtt
    17281 attctggtaa acgagagaaa ttatggcggc ggcggaatct ataatctcta cacaaccgtt
    17341 tcggtggata acaagtttgg cgaatacatt atggtgcatg aaatggggca ccatatgggc
    17401 gcactggccg atgaatatta cacatcttcc atcgcttatg aaatccccgt tgttaagacc
    17461 gaaccgtggg agcccaatat taccgcgctc tttgataaag ataacctgaa atggaaagac
    17521 ctggttgacc ccggtacccc cattcctact ccctggaata aagaaccgtt cgaccgtttt
    17581 ggttacagga tccagcagga acgcgacagc ctgcgtgccg ccaaagtgcc cgaagaactg
    17641 atggaagcgc tttttatgcg ccagatgaag caggaagacg agttctttgc cgctgagaag
    17701 taccgtgaac aggtcggggc cttcgagggt gccggctacc ttgccaaagg actgtaccgg
    17761 ccacaaatcg attgtatcat gtacacacgg catatggaat tctgtaaagt ctgccggcga
    17821 agcctggaag atgtgataaa tatgtattcc aggtagccag tatgaagctg accggattta
    17881 ttttcttccg cttgttctcg ggatttatcg ggctgttgcc ttttcccgta ttgtactcat
    17941 tctccttttt tttatccttc atcttaaaaa gggttattaa atataggaag agaataatac
    18001 ttcaaaatct caccggttgc tttccccgaa tggatgaacg ggccctgaac gatacaatgg
    18061 ggaggtttta cctaaacctg accgatgtta tgctggaagg gctcaaagct tttacaatga
    18121 gcggacggca ggtacttgcc cggtatcgca tccggaatgc agaggttata aaaccttacg
    18181 ttgatgccgg acggagtgtg atctgtgtca caggacatta cgggaactgg gaatggggcg
    18241 ccctgtcacc acctctacag acgcgttttc gcttcatagc tttttataag ccattgaagt
    18301 acaaaaggtt tgactcctat gccaggaata accggtcacg tttcggaaca tatcttgcac
    18361 cgatccggga aacgaccaag acatttgagg aaaaccgggg caatccggcg gtttatctca
    18421 tggcagccga ccagaatccg tcgaaccggg agatggctta ctgggtagat tttttgaaca
    18481 gggaaaccgc ttttctgcat ggcccggaaa aacatgcacg catcaataat tatccggtcg
    18541 tatttgctgc tatccgtcgt acagccagag gttattatga actggaattg tcggttctgg
    18601 cagatgatcc ttctgtttta ccggaagggg agatcacaac ccgttacgcc gcaagcatgg
    18661 aaaagctgat ccgtgaagat cctgccaatt ggttatggtc acataatcga tggaaatatt
    18721 cacgatgaat acatttcttc tatcactgcg atgtgctttt cagtgatcac tttccgttta
    18781 agcttgagtg tactggtcat tgcattgttc tcaatactga atggctcggt cagcaaagtg
    18841 aacttaacta ctcgttcgta aggggtcagc tcctgctgca gctcttccag tttatcccgg
    18901 aatatttttt gaacggtatc gtgggaaacg actttgtgcc tgtcgttgat gtcaatcccc
    18961 aggttttctg cagtcgtttt caggttatcg aatgatggga caatcagcgc cgaaacgaat
    19021 ttccggttat cacccacagc aattacctgt tcgatcagct tttcctgccc gagcaacagt
    19081 tccagttttt gcggggacac atatttgcca accgaagtct tgaacatatc cttca
//
LOCUS       DAOPNH010000020        19113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2775_length_19113_cov_6.263564, whole genome shotgun sequence.
ACCESSION   DAOPNH010000020 DAOPNH010000000
VERSION     DAOPNH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_19113_cov_6.263564"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKZ74_02135"
     CDS             <1..1134
                     /locus_tag="PKZ74_02135"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPM91578.1"
                     /translation="EVKSSTQVKPVNLLDAAFQYYVITKSGLHVSDISLVVLDTGYER
                     EGDLDIRSLFKTISVLEPVLKWQDKIEQSVEDFFFMLKCPVVPETGIGPHCSDPYDCD
                     FKGHCWEHIPDYSIFNISRLSADKKWELYRQGIVKFEDIPSDFRLNVAQWQQVIAELK
                     GETHIDKKAIGRFVSGLSYPLHFLDFESFQPAVPLYDHSHPYQQIVFQYSLHILDSPD
                     ALLQHRYFLAPADKADPRIPFISQLVEDINPTGDILVFNRSFEASRLNEIAATFPNFQ
                     FQISNLTSRIKDLMDIFQKRYYYVPEMKGSYSIKQVLPALAPQFSYEGLAIADGGSAS
                     HAFMALHSEPNPEKIAETRNNLLEYCKLDTLAMVEILNVLQRI"
     gene            complement(1131..2123)
                     /locus_tag="PKZ74_02140"
     CDS             complement(1131..2123)
                     /locus_tag="PKZ74_02140"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM91579.1"
                     /translation="MKCFITGISGFIGASLAEQLIANGHSVNALVRNPEKVSPADHPG
                     IRFFKGDLFDTPVLLQAMEECDAVFHLAAFAKPWSKDPAYPHRVNVGGAVNVFEAALK
                     AGVKKVVFTSSAATMSPSDGDLPVDENTQRTIPYFNAYETTKAEAESKAREYCGRGLP
                     VVMVNPSRVYGPGPLNPSNSTTKMMIGYKSGKWRIIPGDGSKIGNYVFIGDVVRGHIL
                     AAEKGSEGERYILGGENVTFEDFFGILAEVSGRRRKMIHLPVPVMACIARAMEWQARF
                     TGIAPLITEDFVLKYLNDWCLSSAKAERELDYHITPLAEGVRQTLEWDFMKKAD"
     gene            complement(2120..2830)
                     /locus_tag="PKZ74_02145"
     CDS             complement(2120..2830)
                     /locus_tag="PKZ74_02145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPM91580.1"
                     /translation="MEPDTIKAVYAILATAAVSVAYYFVLYRIGKMKKPGTCSVAIGC
                     YLVRKITGFLVLGVVPVAVAWFVFGQSLAESGFRAGTTVTWLPYTALLALFLTVMNYF
                     NSSNIRLQKAYPELRLENWGPGSALVLASGWILYLVGYEMLFRGLLLHTCLEAFGLWP
                     AVIINLSLYFILHLPKGLKESVATIPFGAVVIFLTVKSGSVLPAILLHSIQAVSFELF
                     CIYRNPEMKFNQLISNKL"
     gene            complement(2840..3637)
                     /locus_tag="PKZ74_02150"
     CDS             complement(2840..3637)
                     /locus_tag="PKZ74_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM91581.1"
                     /translation="MKTQETYTLITGASMGLGRELATECARRGQNLLLVALPGSGLSE
                     LRDELKEDFRHHVECYECDLSHEAELEKLAAWVTENYSVDRLINNAGIGGTLRFEEAD
                     AGYLDTIIHLNIRATTILSRLLLPELKRHPSALIMNISSLAAFGPLPYKTIYPASKAF
                     IYSFSRGLSRELIDTGVKVVVVAPGPFISNPDVARRIMKQSFIARLGVLSAREITLKA
                     LKGAEKGQNVIIPGIWNRLNRLLMQLVPEDIRLSVLSNVLKRELPLK"
     gene            complement(3634..4095)
                     /locus_tag="PKZ74_02155"
     CDS             complement(3634..4095)
                     /locus_tag="PKZ74_02155"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPM91582.1"
                     /translation="MKKLTFNPVTLAVNKLKSIKSDPEKVCYGFAFGTFISATPFIGF
                     KWLVALPIVWLAKWNKTACMIGILQVNYLTGPFFYALAYFVGKSVCGYENTFELPDRM
                     TFGAVKDIFFGNADVFVSLLAGGLLLSIPMTLGAFYLARSIFYRKLNTVMS"
     gene            complement(4092..4763)
                     /locus_tag="PKZ74_02160"
     CDS             complement(4092..4763)
                     /locus_tag="PKZ74_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91583.1"
                     /translation="MKTLSFNDKAKHLLILITLLVICLKMMAVTPEEEFRLMKQKNGI
                     DLFYRWMVMPEGNRVRQMKAVFEGGWTKEDIMYLLKNEQQAMAWIPSAREYRNLKEAT
                     AGEWFSYIRFSAPWPLADQDCILQYSLSTEPSGESTIRFTCRPDYIPEVDGITRMKDI
                     NGSFVICPGSGEKGRVECYFISSKASVIPRWITDPIITGSIISLMEGMRGQLSGVEYS
                     QTLQK"
     gene            5232..6101
                     /locus_tag="PKZ74_02165"
     CDS             5232..6101
                     /locus_tag="PKZ74_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPM91584.1"
                     /translation="MLQITTKTIYFERINKVLVYIDRNLGEKMDLEMLASMAHFSPFH
                     FHKIMRAYLRESLGSYIIRVRLDTAASLLIYSREPVSDIAYRIGYDTPAAFTKAFQKR
                     FGTSPSDYRQNKGSLRLEKVELSTFNFKAMNLKPKIKEIKPIPVVYVHAIGDYNNVGP
                     AWEKLAKFMKEKKLFSFGMDFIGVSYDDPSVTETEKLRYDACVTVKKEIRPEGEVGYK
                     VLEGGLYAIFRHTGPYENFSKTYDQIYLNWLPESGYELRNEPPLEFYLNDPDRTRPEN
                     LKTDIYVPVKREA"
     gene            6379..7203
                     /locus_tag="PKZ74_02170"
     CDS             6379..7203
                     /locus_tag="PKZ74_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM91585.1"
                     /translation="MRKIFILSVFATFFTLAGFSQDTVMMQPADSLALADSTLLINHL
                     SDSAAKKIITVYDDYWVTNSIKCGWKTTDTALIGTGLELCDTAHPFVRPTIGKLWRGC
                     TYYHAGWDIGLDYGSPVLAGLNGKVRYARYCSGYGNLVIVRHYSGMEVYYAHLSKIRV
                     KTDQWVEPGDTIGLGGATGRARGNHLHMEFRICDRALDIADMYVHNDTVVNLYKIKEL
                     STKQNQPQTAEYHKVVSGDTLSGIARRYGTTVTNLCTLNSISRETILQIGWRLRVK"
     gene            complement(7280..8074)
                     /locus_tag="PKZ74_02175"
     CDS             complement(7280..8074)
                     /locus_tag="PKZ74_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPM91586.1"
                     /translation="MKLSSSPRLILGAVTGDIIGSVFEWNNVKTTDFQLFNPRTDFTD
                     DSVLTIATMDSILSGMDYAAAYQSYGRNYPGRGYGGYFSSWIHVPDPRPYNSFGNGSA
                     MRVSPVGWAYDNLDEVLYQAKRSAEVTHNHPEGIKGAQATAVAVFMARKGSGKGDIKK
                     YVQDTFGYDLERRIDDIRPGYRFNETCQGTVPEAIIAFLESNDYEDAIRLAISLGGDS
                     DTLACITGGIAEAFYKEIPGWIVKKVLQILPDELMAVIENFSKKFS"
     gene            complement(8080..10002)
                     /locus_tag="PKZ74_02180"
     CDS             complement(8080..10002)
                     /locus_tag="PKZ74_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM91587.1"
                     /translation="MKTLTLLFEESLRKYADNPYIHEKKSTLYESFTFREIGEEVFKF
                     AAGLLCLGLKKGDRVALISESRADWVISELAVLHAGGVSVPLSVLIKEEADLKFRLEH
                     SGTRFVITSGNQAGKIIAAAKDLSLVEKIIFLDPVENKGVAGIYAGDLKQTGEEYLQE
                     HKDRLMEVIHSIGPDDHASISYTSGTTADPKGIILTHRNYTANIEQTLTLLDVPEWWT
                     TLLILPWDHSFAHTVGIYIMMATGSSFAAVQPGRSYLDSLKNIPGNIREIRPVFLLSA
                     PALVKNIRKGIEKGIRDKGKMTEKLFNHALRIAYKYNRTGWDRGKGMRIFLKPLYKLY
                     DLILFRKIRANFGGRLKFFFGGAALLDIEIQKFFYAIGIPVYQGYGLSEASPVISGNM
                     ADRHKLGSSGRVVGHLELKICDEEGRELPAKEKGEIVIRGENVMKGYWKNEKATREAL
                     RDGWLHTGDMGYLDKDGFLFVLGRFKSLLISDDGEKYSPEGIEETITDRSQLIDQLML
                     HNNQDKYTSALLVPNKEALMRWLKERNLAPAHPEGLETLLMEIESEINRYKPGGPFAD
                     LFPARWLPAAIAIIDEPFTIDNALLNITGKVVRARVTEYHRDKFDFLYTPEGKNIVNA
                     RNIEALKKMLNPLDKI"
     gene            complement(10015..11223)
                     /locus_tag="PKZ74_02185"
     CDS             complement(10015..11223)
                     /locus_tag="PKZ74_02185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM91588.1"
                     /translation="MKTAFKWIIPLIITLAVPASCKKDEIGRPAVLNCLLEEDPVYRE
                     NPKSGRFQALIEEYVQKGLPGIVLLVKDDNGFYIGAAGMADIENDIKMQPCHVSKIAS
                     ITKMELGVAILRLQEKGILSLDDPISKYIDKDKLDKIANGNEPITVRNLMNHTSGIYE
                     VIEDAGFYLQILNDPARHWTADGLLKYVYNKEAMFAFDPEDTAGYCNTNFTLLSMIIE
                     AATGKPHSRVLHEEVIDLLGLSDTYYFWHDPLPDDRVAQGYYDLYNNGSIMNLSTWNT
                     GSGNGYGGVYSTVWDMYLFAEALFVEKSLLTQASLDQMLVFHPVVESRKLLGVACFKD
                     FIDIGDPEKDYSIGHRGRDLCYSADLDFFPEHNAIMTMIVNYGTDGDSPLRPVFLEMR
                     DKVAELIIED"
     gene            complement(11259..11852)
                     /locus_tag="PKZ74_02190"
     CDS             complement(11259..11852)
                     /locus_tag="PKZ74_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91589.1"
                     /translation="MKKSIAVVLLFFPLFMQGQDGKAGLFSVAVFNNATLLPPASISA
                     TFNQPIHFGVTLSDEFGWKQAGRHKWFQNAGISYFYHRYVYQAIMLNTQGGYRWMVKG
                     FSMEGYLQAGYMHAFYLTDRSAMQDDGTYSSGMGFGKPQFIAGAGIGLGYDFGKDEQV
                     RRIFVNYDFRLQMPFVSGYVPMLPNGSLGLGFQFNIN"
     gene            complement(11989..12120)
                     /locus_tag="PKZ74_02195"
     CDS             complement(11989..12120)
                     /locus_tag="PKZ74_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91590.1"
                     /translation="MDIQEKQKALDIKAKIDAADKEIDRMVYELYGLTEEDIRIVEG"
     gene            12466..18333
                     /locus_tag="PKZ74_02200"
     CDS             12466..18333
                     /locus_tag="PKZ74_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM91591.1"
                     /translation="MRHRVLLFLACLFLQAGLYAEQLPTINEKSAGFTENKGQFMDQN
                     GDINPSVIYMTDFGGMKVQLHKNGISYELGRIKPQWVDPATIPYRIMTVSDRGDTMVT
                     WPGQAEAMKKLMYHYHRIDIRFAGCNPQTVITPELKETGDGRRMLLEGSSKIAETDRF
                     GQIRYLDIYPGIDMVCYAINSNGGFKYDFILHPGADPSLIRLNYAGADNLVLSDDGIL
                     EIKTRFGSLTESIPLCYSEDAAGVRTPVEGIHYQLSGNTLKFKGTAVQNRKIVIDPAV
                     NLHWAVFFGGDSADYCTASVIDALGNSYLCGSTKSPENIATAGSFQDSLLLPEDGFLT
                     KYSGHGQLLWSTYLNNTPAVDLDIQDDLAILTGNKIMKFDTDGNLLWQINSMHGLKAI
                     CLDRMENIVVVGDSTCPDYGVTFHMDTIVKYNAAGILQWSQSFGGSSTYINDVGLDTA
                     GNIYFVGQTNDSTGIGTENSHLPVYNGTFPSLTGWDYRYELIGNERSGDGFIGKLDPD
                     GQLIFGTYYGGKYFDQVNKISVGDNGIFVIAGETNSRTGISTTGAYHEELNPQGRYGF
                     MKIAIGGTFECDPGWPLIDTCIIGSYFPTPYDWRCQSTDAFIAKFSADGTRLWGTYYG
                     DNASDFSTCVNTTSNCDTISLGGISPANFCIVNPSDCPPARLASTYSYLHANFNYGEG
                     NYAYLAQLDSNGHRIWGTYFPKETDIPPDNAKRFSVSDVNHIKREIFVAGTNYSLWSL
                     NYPQNAYLWAFNTADIDTIFQPGHASECYGDSTVLRLGILTEPLADLGFQWMFKGLPV
                     EGATDSVLVIQNTSPSDTGFYYCHITQGEFSWKSDSVNVAVRENPAFNKFSDNDQDLM
                     LPDGRLLADMDNNGDYDAICKQKIIYNDSLSFIQNDPISLIYADVSVIDILNDQDLFI
                     FTHDPLCSNNPECTRPSKLQRIKNGHQEIYESEILDRVVTWADFDNDGKPEGIAMKFG
                     QQGLDIFYWVYLVEFENDTVYETVAGQVFFGNYYTLEPEVDAADYDADGDIDLLITDI
                     PVPGTPYRSTSILLNTGGVFQELPIPARQNHSQGKIQWFDADSDNDLDFLFNYLIYTN
                     YSPWIAMVLSTYTHNEGGFELESSDTIPVSEQYNNPKPIVLDYDNNGSTDIVFKTSIL
                     KNNNDNYELTPSTYNPAHTGLTEDAMSFGDFDNDGDLDILGKNAIFTNDVCNVNNLPS
                     APPGFLSNVTNHGALFSWQKSTDLETPQMGLTYNLRVGTTPGGNEIMSSMSDSTGYRK
                     VARMGNVYQNTSWWLKDLQPGKYYCSVQAIDNAYAGGPFSEEQIFIISDTIIQPVNTC
                     SCIGNATTFITGVILHPHIELSYQWYKDDQIISGATDSIYIIPVTSVNDTGSYYCMIN
                     GGEFHCKTDVVSLSIREYLAFDNIDIWGQNSYTSLFGDINDDRKPDIFNAGGNQSAES
                     RLWINNGTGFTEQNSSVHEFYNPSAAFGDFDGDGDLDLTIAGDTSAGASYKIPVIFIY
                     RNDAGTFHPTANNFTGGRNGALKWGDFDNDGDLDLVISGYDPNYGVNLCFYRNDNGEF
                     NRYQCFSNDGVEYSSIDLADYDQDGDIDVLAGGGNLWDGSSRVLRLFENNSGNFTMLN
                     AGLEGLFNCAAVWGDFDSDEDLDIVLNGSMTLNSGDTTFLYRNDAGTFVLVPDQIPGS
                     SQEINFGDYNNDGILDIVAGNKYLENINNNFVLQPTSYSTAHSGITSGGNKPGDYDND
                     GDLDLPGWTNVCRNDVCNPANTPPTAPSGLSVIISNDTVTFSWQPSADLETPQPGLTY
                     NLRVGTTPGGNEIMPSMSDSTGWRKVAQMGNVYQNTTWWLHDLQPGTYYWSVQAIDNS
                     YAGGPFAQEQLFSIYALSTTWTGAIDSLWSRADNWSDGLPGENTTVIIPGEPVNQPVL
                     NISTANKKLILQTGSELIINSSKLLNLSDSLIINQHSPADSTKLIIRGNIDIIRE"
     gene            18606..>19113
                     /locus_tag="PKZ74_02205"
     CDS             18606..>19113
                     /locus_tag="PKZ74_02205"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91592.1"
                     /translation="MAKIKPQFKLKSSKPVDVATLIYLIIYYNNKRFKCSTGEKIEPR
                     YWDSVSQRAMMGTGEKTRDQDHADINFQLNKHHNQAIIIFDRFEREGKIPSLKTVKQE
                     LDKGLYVEEKKEPKGRISLNKYIDQFIKDIEKGKRLTDKGLKYSHGTIKNYKGFQTQF
                     DEFQQETHR"
BASE COUNT         4991 a         4484 c         4646 g         4992 t
ORIGIN      
        1 gaagtcaaga gctccacaca ggtaaagccg gtgaacctgc tggacgccgc tttccagtat
       61 tatgtcatca caaaaagcgg gttgcatgtt tccgatattt cactggttgt gctggataca
      121 ggctacgaaa gagagggtga tcttgatatc cgttctctct tcaaaaccat aagtgtactg
      181 gaaccggtgc tgaaatggca ggataagatt gaacaatccg ttgaagactt cttcttcatg
      241 ctgaaatgtc ccgtggtacc ggaaaccggc atcggtcctc actgcagcga tccgtacgac
      301 tgtgatttca aagggcattg ctgggagcat attcccgact attccatctt caatatttcc
      361 cggcttagcg ccgataagaa atgggagctt taccgccagg ggatcgtgaa gtttgaagat
      421 attccttcgg atttcaggct gaatgtagcc cagtggcaac aggtcatcgc ggagttgaaa
      481 ggggagacgc atatcgacaa aaaagcgatc ggccgctttg tctccggcct cagttatccg
      541 ctccatttcc tcgatttcga atcgttccag ccggcggtgc cgttgtacga tcacagccac
      601 ccttaccagc aaatcgtctt tcagtattcg ctgcatattt tggattcacc ggatgccctg
      661 ctgcaacacc gctatttcct ggccccggcc gataaagcgg acccgcgaat cccatttatc
      721 tcccaattgg tagaagatat aaaccctacc ggcgatatcc ttgttttcaa ccgctctttc
      781 gaagcctcca ggctgaacga aatcgccgcc actttcccga atttccagtt tcaaatttcg
      841 aatctgacct cccgaattaa agacctgatg gacatcttcc agaaacggta ttattacgtc
      901 cccgaaatga aaggttctta ttccattaag caggtgcttc ccgcattggc gccgcaattc
      961 agttatgaag gcttggccat tgccgacgga ggctcggcca gccacgcctt tatggccctg
     1021 cattccgaac ccaaccctga gaaaatcgca gaaacccgga ataacctgct tgaatactgt
     1081 aaactggata ccctggccat ggtggagatc ctgaatgttt tacagaggat ctaatcagct
     1141 tttttcataa aatcccattc cagggtctgc ctgactccct cagccagagg tgttatatgg
     1201 taatccagtt cccgttctgc ttttgcgctg ctcaagcacc aatcgttgag atatttaaga
     1261 acaaaatctt cggtgatcag cggagcgatg ccggtaaacc gggcctgcca ttccatggcc
     1321 ctggctatac atgccataac gggtacgggt agatggatca ttttacgcct tcttccggaa
     1381 acttctgcca aaattccgaa aaagtcttcg aaggtaacat tctcgccgcc caggatatac
     1441 cgttcacctt cactcccttt ttcagcggca agaatatggc cgcggaccac gtcaccgata
     1501 aaaacataat tcccgatttt cgaaccatca cccggaatta tcctccattt ccccgactta
     1561 tatccaatca tcattttagt ggttgaattg ctcggattta agggtcccgg gccgtatacc
     1621 cttgatggat tcaccatgac gacgggcaaa cccctgccac agtattccct tgcttttgat
     1681 tctgcttctg cttttgtcgt ttcatacgca ttgaagtacg ggattgtccg ttgagtattt
     1741 tcatcaacgg gaaggtcgcc gtcggatgga gacattgttg cggctgaaga ggtgaacaca
     1801 acctttttga caccggcttt tagcgcagct tcaaaaacat taacggctcc tccgacattc
     1861 acgcgatgcg gataagccgg atctttcgac cagggtttgg cgaaggcagc caggtgaaag
     1921 actgcatcgc attcctccat ggcctgcaac agaacagggg tatcaaacag gtcgccctta
     1981 aaaaaccgga tgcccggatg atcagcaggg gatacttttt caggattccg gacaagtgca
     2041 ttgacggaat gcccgtttgc aatcagttgt tcagccaggg aagcgccgat aaatccggat
     2101 ataccggtga taaagcattt cataacttat tagatattag ttggttgaat ttcatttcgg
     2161 ggttacggta aatacagaat aattcaaatg aaacggcctg tatggaatga aggagaatag
     2221 ccggtaaaac agacccggat ttaacggtca ggaaaatgac gacagccccg aaggggatcg
     2281 ttgccaccga ctctttcagc cctttaggca gatgaaggat aaagtacagc gacaggttga
     2341 tgatcacggc cggccacaaa ccgaatgctt ccaggcaggt atgcagaagc aaacccctga
     2401 acagcatctc atatcccacc aggtacagga tccacccgga ggcgaggacc agggcggagc
     2461 ccggacccca gttttcgagg cggagctcag gataggcctt ttgcaacctg atgttggatg
     2521 aattgaagta attcatgaca gtcagaaaaa gtgcaagtag agctgtataa ggcagccagg
     2581 taaccgttgt cccggccctg aaacctgatt cagccaaact ctgaccgaaa acgaaccagg
     2641 caacggctac aggcacaacg ccgaggacca ggaaaccggt gattttcctt accaggtaac
     2701 aaccaatggc cacgctgcag gttcccggtt tcttcatttt accgatccgg taaagcacga
     2761 agtaataagc cactgaaacg gctgcggtgg ccagaattgc ataaacggct ttgatagtat
     2821 ccggttccat ggcaaatgtt tatttaagcg gcagttctct tttcagtaca ttgctcaaaa
     2881 cgctcaggcg gatgtcttca ggcaccagtt gcatcagcag ccggtttaac ctgttccaga
     2941 taccggggat gatcacgttc tgacctttct cggcgccttt cagcgccttc agcgtaattt
     3001 cccgggcgga gaggacgccc agccgggcga taaagctctg cttcatgatc cgcctggcca
     3061 catcggggtt ggagataaat ggcccgggcg ccaccacaac cacttttaca ccggtatcta
     3121 tcaattccct gctcaagccc ctggagaagg aatagatgaa tgccttggag gcaggataaa
     3181 tggttttgta tggcaacggg ccaaaggcgg ccaggctgga gatattcatg atcagtgcgc
     3241 tgggatggcg tttcagttca ggcaataaaa gccggctcag gatggtggtt gcccggatgt
     3301 tcagatggat gatcgtatcg agataaccgg catcggcctc ttcaaaccgg agagtgcccc
     3361 cgatccccgc gttattgatc agccggtcga ccgaatagtt ctctgtcacc caggccgcca
     3421 gcttttccag ctccgcttca tggctcagat cacattcgta acattcgaca tggtgccgaa
     3481 agtcctcttt cagctcgtcg cgcaattccg ataagccgct gccaggcagc gcgaccagca
     3541 gcagattctg gcccctccgg gcacattcgg tcgccagttc tcttcccagc cccatactgg
     3601 cgccggtaat cagggtatag gtctcctgtg ttttcatgac ataacagtat ttaatttcct
     3661 gtaaaatatc gaccgggcca ggtaaaaagc cccgagagtc atggggatgc tgagaagcaa
     3721 tccgccggca agcagggata caaacacatc cgcattaccg aaaaagatat cctttaccgc
     3781 tccgaatgtc atccggtcgg gtaattcaaa cgtattctca taaccacaga ccgatttacc
     3841 gacaaagtat gccagggcat agaagaacgg cccggtaaga tagttgacct gaaggatacc
     3901 gatcatgcag gcggtcttgt tccatttggc cagccacacg atgggcagtg ccaccagcca
     3961 tttgaaaccg atgaacggcg tggcgctgat gaaggtgccg aaagcaaacc cgtaacagac
     4021 cttttccgga tccgatttga tgcttttgag cttgttgacg gccagagtaa cgggattgaa
     4081 ggtgagcttt ttcatttttg cagggtttga gaatattcaa ctccggataa ttgaccgcgc
     4141 atgccttcca tcaggcttat gatgcttccg gttataatag gatctgtgat ccagcgcggg
     4201 atgacagaag ccttggagga gatgaagtaa cattcaaccc tccctttttc gccgcttccg
     4261 gggcagataa cgaacgaacc gttgatgtcc ttcatccggg taatcccatc cacctcggga
     4321 atgtaatcgg gccggcaggt aaaccggatc gtcgattcac ccgatggttc tgttgataaa
     4381 gaatactgaa gaatgcaatc ctggtcggcc aggggccagg gcgcgctgaa gcggatatag
     4441 ctgaaccatt caccagccgt cgcctctttc aggttgcggt actcccgggc agatgggatc
     4501 caggccatgg cctgctgttc gtttttaaga aggtacatga tatcctcctt tgtccagcct
     4561 ccttcaaaga ccgccttcat ctgcctaacc cggttgcctt cgggcatgac catccagcgg
     4621 taaaacaggt ctatcccgtt tttctgcttc attaaccgga attcttcttc cggtgttacg
     4681 gccatcatct tcaggcaaat cactaataag gtgatcagga tcagaaggtg ctttgctttg
     4741 tcattaaatg atagtgtttt catagctctt gttttttgat ccaaaattat atcctgcaaa
     4801 tgccttaacg aaaaggcttt atgccatggt aagcctgaaa atttgcagtt ggtaaaaatc
     4861 aatcttcccc gggaaggtgt ttttatggaa ttctaattct aaagacaggg gaaatcttaa
     4921 aaggtctatt ttttgcaaat caatttttga tgagttgcaa aatttcccgg aaataatggg
     4981 catttttgct gttaaagcaa atcttatgtt aatatcgcaa gaatggaaaa gcggggttat
     5041 aatacgggaa gtagatttgt ggatatttag aatttgtgat attgtgatat tttgaacttg
     5101 tgattttttg gtattgtgag cttgtgagct tttgattttt tgatattgtg agcttgtgag
     5161 cttttgaact tttgattttt tgatattgtg aacttgtgat tttttaaccg gtaaccctta
     5221 acaatagcgc gatgctacag ataactacca agaccattta ttttgagcga attaacaagg
     5281 tgcttgttta catcgaccgg aaccttgggg aaaagatgga tctggagatg ctggcgtcga
     5341 tggctcactt ctcgccgttt cattttcaca agatcatgcg ggcgtatctg cgcgaatccc
     5401 tcggctcgta tatcataagg gtcaggctcg acacagccgc ttccctgttg atatattcca
     5461 gggagccggt ttcggatatc gcctaccgga tcggttatga tacaccggcc gcctttacca
     5521 aagcttttca gaaacgtttc ggtacgtcgc ccagcgacta tcgtcaaaat aagggatccc
     5581 tgcgattaga aaaagtagag ttatccacat ttaattttaa agcgatgaat ttgaaaccta
     5641 agatcaaaga gatcaagcct attccggtgg tatatgtcca tgccatcggg gattacaaca
     5701 atgtcggccc ggcatgggaa aagctggcga agttcatgaa ggaaaagaag ttgttctcct
     5761 tcgggatgga tttcatcggt gtttcgtatg atgatccttc agtcacagag acggagaagc
     5821 tcaggtatga tgcctgtgtt acagttaaga aagagatcag gccggaaggg gaggtcgggt
     5881 ataaagtcct cgaaggagga ttgtatgcga ttttccgcca taccggtccg tatgagaatt
     5941 tttcgaaaac ttacgaccag atctatttga actggttgcc ggaaagcgga tatgaacttc
     6001 gtaacgaacc gccgctggag ttctacctga acgatcccga tcgcacccgg ccggagaacc
     6061 tgaagacgga tatctacgtg ccggtgaaac gtgaggcgtg agcagtgaag ggtgagcagt
     6121 gaacagaaaa aactctgggg ccctctgtaa ttttaacatt aattcgtgta aattctttgt
     6181 attcgcgtct attcgtgttc tattaatagc acacgaatga cgcgaattta tacgaatgaa
     6241 cacggatctt tcagatcaaa ccacagacac gttgatagaa attttcaaca aaaactgtta
     6301 cttttggccc gaaaatgtta aattatccaa gttatcccgg ggtacaaagc gatgagtgaa
     6361 tttccaatta ggcgagcgat gcggaagatt ttcattttgt ctgttttcgc cacttttttt
     6421 acccttgcgg gcttttcgca ggatacggtt atgatgcaac cggcggattc cttggcatta
     6481 gccgacagca cacttcttat caaccactta tccgacagtg ctgcaaaaaa gatcatcact
     6541 gtttacgacg attactgggt gaccaattcc atcaaatgcg gctggaagac gaccgatacc
     6601 gccctgatcg gaacaggcct ggagctgtgc gatacggcac atccattcgt caggccgact
     6661 atcgggaagc tttggcgggg atgcacatac tatcatgccg ggtgggatat cgggcttgat
     6721 tacggttcgc cggtattagc cgggcttaac gggaaggtgc gttatgcaag gtattgcagc
     6781 ggatacggca acctggtcat cgtgcggcat tacagcggga tggaggtcta ttatgcccat
     6841 ctttcgaaaa tacgggtaaa gaccgatcaa tgggtggaac cgggagatac cattgggctc
     6901 ggaggcgcta cgggacgcgc cagggggaac cacctgcaca tggagttcag gatttgtgac
     6961 cgggccctcg acatcgccga catgtatgtc cacaacgata cagtcgtcaa tctttacaag
     7021 atcaaggaac tctccacgaa gcaaaaccag ccgcaaacgg cagaatatca caaagtggtg
     7081 agtggggata ccctatccgg aattgcaaga agatacggga ccaccgtcac caatctgtgt
     7141 acgctgaaca gcataagccg ggaaaccatt ttgcagatcg ggtggcggct cagggtgaaa
     7201 taacgagttc aaagttcaaa gtgcaaagtt gaactggctg aggtagcaat gaaaggcaat
     7261 acggcccggc tgtagggttt caggagaatt tcttcgagaa attttcaatg actgccatca
     7321 gctcgtccgg caggatttgc aagacctttt ttacgatcca gcctggtatt tccttgtaga
     7381 aagcttcggc gatgccgccg gtgatacacg ccagcgtatc gctgtcgccg ccgagggaaa
     7441 tggccaaccg gatggcgtct tcgtagtcgt tgctttcgag aaaggcgatg atcgcttcgg
     7501 gcacagttcc ctggcatgtt tcgttgaacc ggtaaccggg ccggatgtca tcaattcttc
     7561 tttccaggtc ataaccaaac gtatcctgga catacttttt aatatcccct ttaccggaac
     7621 cctttcgcgc catgaacacg gcaacggcag tagcctgcgc ccctttgatc ccctccgggt
     7681 ggttatgggt aacctccgcg cttcttttag cctggtacag gacctcatcc aaattgtcat
     7741 acgcccagcc gacggggctc acccgcattg ccgagccatt gccgaagctg ttgtaaggcc
     7801 tgggatccgg aacatggatc caggagctga aataaccgcc ataaccacgt cccgggtaat
     7861 tccgtccgta agattggtag gccgcagcat aatccatacc ggagaggatg ctgtccattg
     7921 tggcaatggt gaggacgctg tcgtcggtaa aatctgtccg gggattgaat aactgaaaat
     7981 cggtggtttt cacgttgttc cattcaaaga ccgacccgat gatatcgccg gtaacagcgc
     8041 ccaggataag gcggggagag gaggagagtt tcatttcttt taaattttat ctaatggatt
     8101 aagcatcttc ttcagcgcct caatatttct tgcattgacg atattcttcc cttcgggggt
     8161 gtataaaaag tcaaatttat cccggtgata ttcagttact ctcgccctga cgacttttcc
     8221 cgtaatgttg agcaaagcat tgtcgatcgt aaagggttcg tcaatgatag cgattgcggc
     8281 cggcagccac cgggcaggga atagatcggc aaaggggccg cccggcttgt accggttgat
     8341 ttccgactcg atttccatca gaagggtttc caacccttca ggatgggcgg gtgctaaatt
     8401 cctctccttc agccaccgca tcagggcttc tttattcggg accaggagtg cggaggtgta
     8461 tttgtcctgg ttgttgtgca gcatcaactg gtcgatcagc tgcgatcggt cggtgatcgt
     8521 ctcttctatg ccttccgggc tgtatttctc cccgtcgtcg gaaatgagca ggctcttgaa
     8581 ccggcccagg acaaaaagaa agccgtcttt atccaggtaa cccatatccc ccgtgtggag
     8641 ccagccgtcg cggagcgcct cccgtgtcgc tttttcattc ttccagtagc ccttcatgac
     8701 gttctccccc cggatgacaa tctctccttt ctccttagcc ggcagttctc tgccctcttc
     8761 atcgcagatc ttcagttcaa gatgcccgac gacccggccc gaagagccga gcttgtgccg
     8821 gtctgccatg ttcccggaga tgacgggaga ggcttccgac aggccatagc cctggtaaac
     8881 gggaatccct attgcataga agaacttctg gatctctatg tcgagcagcg cggctccgcc
     8941 gaaaaagaat ttcagcctgc cgccaaaatt agccctgatc tttctgaaca gtattaaatc
     9001 gtataatttg tacaacggct tgagaaagat tctcatccct ttcccgcggt cccatccggt
     9061 tctgttatat ttataagcaa tccgcagtgc gtggttgaac agtttttcgg tcatttttcc
     9121 cttgtccctg atcccttttt cgatcccttt gcggatattc ttgaccaggg ccggcgcact
     9181 cagtaaaaat accggcctga tctcgcggat gttccctggg atattcttca ggctgtcgag
     9241 gtacgacctg ccaggttgca cggctgcaaa gctcgatcct gtggccatca taatgtagat
     9301 ccctaccgta tgcgcaaaag aatggtccca cggcaggatc agcagggtgg tccaccattc
     9361 cgggacatcc agcagcgtca gcgtctgttc gatgttcgcc gtgtaattgc ggtgcgtgag
     9421 gatgatccct ttcggatcgg ccgtggtccc ggaggtatag ctgatgctgg catgatcatc
     9481 cggcccgatc gaatggatga cctccattaa ccggtctttg tgttcctgca ggtattcttc
     9541 ccccgtttgc ttcagatccc cggcatatat cccggctacc cctttgtttt caacaggatc
     9601 gaggaagatg atcttttcga ccaatgagag atctttagcg gcagcgatga tcttcccggc
     9661 ctggttgccc gaagtgatca caaaccgggt ccctgagtgc tccagcctga atttcaggtc
     9721 ggcttcctcc ttgatcagaa cagagagcgg gacgctcacg ccgccggcgt gcagcacagc
     9781 gagttcactg atcacccagt cggcgcgcga ttcggagatc aaagccaccc tgtcgccttt
     9841 ttttaagccc aggcacagca atccggccgc aaatttaaaa acctcttccc cgatctccct
     9901 gaaggtaaaa gactcataaa gagtcgattt tttttcatgg atgtagggat tgtccgcata
     9961 cttccggaga ctttcctcaa aaagaagggt cagtgttttc ataggacagg cgttttaatc
    10021 ttcgatgatc aattcagcca ctttatcgcg catctccagg aaaaccgggc ggagagggct
    10081 gtcgccgtcc gttccgtagt tgacgatcat ggtcataatg gcattgtgtt ccgggaagaa
    10141 atcgaggtcg gcgctgtagc acagatcgcg gccccggtgg ccgatggaat aatccttctc
    10201 cgggtcgccg atgtcgataa aatccttgaa gcaggccact cccaaaagct tacgcgattc
    10261 caccacagga tggaacacca gcatctggtc gagcgaagcc tgtgtcagca gcgacttttc
    10321 gacgaagaga gcttctgcga aaaggtacat gtcccagacc gtgctgtaaa ccccgccgta
    10381 accgttgcca ctgccggtgt tccaggtcga caggttcatg atcgagccgt tgttgtaaag
    10441 atcatagtat ccctgggcca cccggtcgtc cggcagggga tcgtgccaga aataataggt
    10501 atcgctcagc ccgagcaggt cgatcacctc ctcatgcagc acccgggaat gcggtttgcc
    10561 tgtagcggct tcgatgatca tgctcagcag ggtgaaattg gtgttgcaat agcccgccgt
    10621 atcctccgga tcaaaagcga acatcgcctc tttgttgtaa acgtatttca gcaggccatc
    10681 tgcggtccag tgccgggcag gatcgttcag gatctgaagg taaaagccgg catcttcgat
    10741 cacttcgtag attccgctgg tatggttcat caggttcctg acggtaatgg gttcattgcc
    10801 gttagcaatc ttatcgagct tatccttatc gatgtattta ctgatcgggt catcgaggga
    10861 aaggatccct ttttcctgca accggaggat cgctaccccc agttccatct tggtgatgct
    10921 ggctattttg gagacatggc atggctgcat tttgatgtcg ttttctatgt cggccatgcc
    10981 ggcggcaccg atatagaatc cattatcgtc tttcaccagc aaaacaatgc ccgggagccc
    11041 cttttggacg tattcttcga tgagggcctg gaaccggccg cttttgggat tttcgcggta
    11101 aaccgggtcc tcttccagca ggcagttcaa aacggcaggc cttccgatct catctttttt
    11161 gcacgatgcc ggcacagcca acgttatgat cagggggatg atccatttga aggcggtttt
    11221 catagcatta cccgattttc agttgtaaaa ggcaacattt aattgatatt gaactggaag
    11281 cccagtccga gcgacccgtt cggcagcatg ggcacgtagc ccgacacgaa cggcatctga
    11341 aggcggaagt cgtagttcac gaagatcctt ctgacctgtt catccttacc aaaatcatac
    11401 cccagcccga taccggcgcc ggcgatgaat tgcggtttgc cgaaccccat gccggagctg
    11461 taggtgccgt catcctgcat tgccgaccgg tcggtgaggt agaaagcatg catatatccg
    11521 gcctgcaggt atccttccat cgagaacccc ttgaccatcc accggtagcc accctgggta
    11581 ttcagcatga tggcctggta gacgtagcgg tgatagaaat agctgatacc cgcattctgg
    11641 aaccatttgt ggcggccggc ctgtttccag ccaaactcat ccgacagggt aaccccgaag
    11701 tggatgggct ggttgaacgt ggcggagatc gaggccggcg gcaggagggt ggcgttattg
    11761 aaaacggcca ccgaaaaaag ccctgctttc ccgtcctggc cttgcatgaa caaagggaag
    11821 aacagaagaa caacagcgat tgatttcttc atgatgaaaa gataaagcgg ttacaacaaa
    11881 tttagggatt taagtaaaac gggaatggaa agctcccctt ttttcgatcg gttaaaacca
    11941 ggtttcagga agggccacga aggacccgct ttgtttgaaa atggcttatt atccttctac
    12001 gatccggata tcctcctccg tcaacccata caactcataa accatccggt cgatctcttt
    12061 atcggcggcg tctatctttg ccttgatatc cagcgctttc tgcttctcct gaatatccat
    12121 attgtggaaa tgagcccgga acctcgcata agccgcttcc aggctcgttt tgtcctgcgt
    12181 tttaagatat ttgctgacga cggaagattg gaagaggggc ttataaaggg tagatttaaa
    12241 aggccgaagg taagaaaaat accagtattg tataattgag tgttgaatgt tgaaaattaa
    12301 atgagaagag tataatatta taaatccggg aaatacagaa tgctaattga atgttaagaa
    12361 ataatcctac ctttgacaca atgggaattt aaagagatca tcaacctaac catccaggat
    12421 tttattcatt tctttcattt ttattgattt atataagaaa gcaatatgag acaccgagtt
    12481 ctattgtttt tagcatgcct gttcctgcaa gccgggctct atgctgaaca attgcccacc
    12541 ataaatgaaa agtcagcggg ctttactgaa aataagggcc aattcatgga tcaaaatggt
    12601 gacatcaatc catccgttat ttacatgact gactttggag gcatgaaagt acagctccat
    12661 aaaaatggaa tcagttatga gttgggccgg ataaagcccc aatgggttga tcccgcaaca
    12721 attccgtaca ggatcatgac ggtcagcgac cggggcgata cgatggtaac ctggcccgga
    12781 caagcagaag cgatgaaaaa acttatgtac cattatcacc gcattgatat caggttcgct
    12841 ggttgtaatc cgcagacagt tattactcct gaactgaagg agacaggcga cggcaggagg
    12901 atgctactgg aaggctcttc aaaaatcgct gagacggaca ggtttggcca gatccgttac
    12961 ctggatatct atccggggat tgatatggtt tgctacgcga tcaactcaaa tggtggtttc
    13021 aaatatgatt ttatcctgca ccccggtgcc gacccttccc tgatcaggct taactatgcc
    13081 ggcgctgaca atcttgtact cagcgatgat gggatccttg agattaaaac ccgctttgga
    13141 agtctgacgg aaagcatccc gctgtgttat tctgaagatg ctgcaggtgt cagaacccca
    13201 gttgagggca ttcattacca gctatcaggg aatacgctaa aattcaaagg aactgcggta
    13261 caaaaccgga aaatagtgat tgatccggcg gtaaatctgc actgggctgt cttttttggc
    13321 ggtgatagtg cggactattg cacagcgtca gtaattgatg ctttgggcaa ctcttattta
    13381 tgtggaagta ccaaaagccc tgaaaatatt gcaactgccg gaagtttcca ggacagccta
    13441 ttgttgcccg aggatggctt tttaacgaag tactctggtc acggacaatt gctctggagc
    13501 acctacctga acaacacccc ggctgttgac ctggatattc aagacgacct ggccattttg
    13561 accggcaata agatcatgaa atttgatact gacggcaact tattatggca gatcaattcg
    13621 atgcatgggc tgaaggccat ttgcctggac cggatggaga atatagtagt cgtgggcgac
    13681 agcacttgcc ctgattatgg ggtgacattt catatggata caatagtaaa atataatgct
    13741 gcgggtattc tgcaatggtc acaaagcttt ggcggaagta gtacatatat taatgatgtc
    13801 ggtttggata cggcagggaa tatctatttt gtaggccaga caaacgattc gaccggtatt
    13861 ggtacggaaa attcacatct gcctgtttat aacggcactt ttccaagcct gaccggatgg
    13921 gattacaggt atgagcttat tggaaatgaa agatcaggag atggctttat tggaaaactt
    13981 gatcccgatg gccaattgat ctttggaact tattacggcg ggaaatattt tgaccaggtc
    14041 aacaagattt cagttggtga taatggcatt tttgtaattg ccggtgaaac gaatagccgg
    14101 accggtattt caacaacagg agcttatcat gaagaattaa atccccaggg cagatatggg
    14161 tttatgaaaa ttgcgatagg gggtacattt gaatgtgatc cgggctggcc gcttattgat
    14221 acatgcatca ttggatccta ttttccaaca ccttacgatt ggcgatgcca gagtactgat
    14281 gcatttatag ctaagttttc tgctgacgga acccgtttgt ggggaactta ttacggggac
    14341 aatgccagtg atttcagcac ttgtgtaaat accacatcaa attgtgatac aatttcttta
    14401 ggtggaattt cacctgcaaa tttttgcatc gtcaacccgt cggattgccc tcctgcccgg
    14461 ttggcttcta cttattccta tctgcatgcc aatttcaatt atggagaagg taactatgcg
    14521 tatttggccc aactggattc aaacgggcac cgtatctggg gaacctattt tcccaaagaa
    14581 acggatatcc cgcctgataa tgccaaacgg ttttcagtat cagatgttaa ccatataaag
    14641 cgggaaatat ttgttgcagg cacaaattac agcctctggt ctttgaacta cccgcagaac
    14701 gcttatctct gggcctttaa tacggctgat atcgatacga tatttcaacc cggccatgcc
    14761 agtgaatgtt atggtgatag tactgtgcta agactgggta ttttgactga acccctggct
    14821 gaccttggtt ttcaatggat gttcaaaggg cttccggttg aaggtgcaac agacagcgtt
    14881 ctggtcatcc aaaacacctc tccatcggat actggctttt attattgcca tattacccag
    14941 ggcgaatttt cctggaaatc ggatagtgta aacgtagccg taagggaaaa tcctgccttt
    15001 aataaatttt cagacaatga tcaggatttg atgctgcccg atggaagact tttggctgat
    15061 atggataata atggcgatta tgatgcgatc tgcaagcaga aaatcatcta caacgactcg
    15121 ctttcattta tacaaaatga tccaatctcc ttaatctatg ccgatgtgtc tgtgattgac
    15181 atcctgaatg atcaggacct gtttatcttt acacatgatc cgttatgttc aaataaccca
    15241 gaatgcacaa ggccgtcgaa actacagcgt attaaaaatg gccatcagga gatttatgag
    15301 agcgaaatcc ttgatcgtgt ggtaacctgg gctgattttg acaatgatgg caagccggag
    15361 ggtatagcta tgaaatttgg ccaacaaggg cttgatatat tttattgggt ttacctggtt
    15421 gaatttgaga acgataccgt ttatgaaaca gtggcaggcc aagtattctt cggtaattat
    15481 tatacacttg agccggaggt agatgctgca gattatgatg ctgatggtga tatcgacctg
    15541 ttaataactg atattccagt tccagggacg ccatacaggt caacttcaat cctgttgaac
    15601 acggggggtg tttttcagga attgcccata ccagcacgcc aaaatcatag ccagggtaaa
    15661 atacagtggt ttgatgcaga ctcggataat gacctcgatt ttctcttcaa ttacttaatt
    15721 tacaccaact atagtccctg gatagcgatg gtcctgagta catatactca taatgaaggg
    15781 ggttttgaac tggaatcttc tgataccata cctgtttcag aacagtacaa taatccgaaa
    15841 cctattgtcc tggattatga taataacggt tcgacagaca tcgtgttcaa gacatcgata
    15901 ctcaaaaata ataatgacaa ttacgaatta acaccctcaa cctataatcc ggctcatacc
    15961 gggctgaccg aagatgccat gtcttttggt gattttgaca atgatggcga ccttgacatc
    16021 ttgggcaaaa atgccatatt cactaatgat gtgtgcaatg ttaacaatct cccctcagcc
    16081 ccgccgggct tcttatccaa tgttacaaac catggggcac tctttagctg gcagaagtca
    16141 accgatttgg aaacaccgca aatggggctg acctataatc ttcgggtcgg tacaacacca
    16201 ggtggcaacg agatcatgtc gtccatgtcc gattcaacgg gctaccgtaa ggtggcccga
    16261 atgggaaatg tttatcagaa tacaagctgg tggttgaagg atttgcagcc gggtaaatat
    16321 tactgcagcg tgcaagccat cgacaacgca tacgctggtg gtcctttttc cgaagaacag
    16381 attttcatta tttccgacac catcattcag ccggtaaaca cgtgttcctg cattggtaat
    16441 gctacgacct ttattaccgg agttattctg cacccacaca tcgaattatc ctatcagtgg
    16501 tataaggatg atcagataat ctcaggggcc accgacagca tttatatcat cccggttacc
    16561 tcagttaacg ataccggcag ctattactgc atgatcaatg gaggtgaatt tcattgcaaa
    16621 accgatgtgg ttagcctttc aataagagaa tatcttgctt ttgacaacat tgatatctgg
    16681 gggcagaact cctatacaag cctgtttggt gacatcaatg atgatcgcaa acctgatata
    16741 tttaatgcag gtggaaatca aagcgctgaa agcaggttat ggatcaataa cggaactggt
    16801 tttacagagc aaaattcaag tgttcatgaa ttttataatc cttcagcggc ttttggcgat
    16861 tttgacggag atggggattt ggatttgacc attgccggcg atacaagtgc aggtgcttca
    16921 tataaaatac ctgttatctt tatttataga aatgatgccg gaacttttca tccgacagca
    16981 aataatttta ccggcggcag gaatggcgct ttaaaatggg gggattttga taacgatggc
    17041 gatctggacc tggtaatttc aggatatgat cctaattatg gcgtcaattt gtgtttctat
    17101 agaaacgata atggtgaatt taatcgatat cagtgttttt caaatgatgg cgtggaatac
    17161 agttctattg atcttgctga ttatgatcag gatggagata ttgatgttct tgcaggaggc
    17221 ggaaatctat gggatggatc gtcaagggtg ctgcgtttat ttgaaaacaa tagcgggaat
    17281 tttaccatgt taaatgccgg gctggaagga ttattcaact gcgctgcggt ttggggagat
    17341 tttgacagcg acgaggacct ggatatcgtt ttgaatggct ctatgacttt gaatagtggc
    17401 gatacaactt ttctataccg gaatgatgcg ggcacatttg ttttagtgcc agatcaaatt
    17461 cccggatcca gccaggagat caattttgga gattataata atgatggcat cctggatatt
    17521 gttgccggaa acaagtacct ggagaatatc aataataact ttgttctgca gccgacatca
    17581 tattcaacag cgcattcagg gatcacttcc gggggaaaca agccgggaga ttacgacaat
    17641 gatggcgacc ttgatttgcc ggggtggact aatgtatgcc ggaacgatgt ttgtaatcct
    17701 gcaaatacgc cgcctaccgc cccgtcagga ttgtcagtta tcatctccaa cgataccgtc
    17761 accttctcct ggcagccatc tgctgatctt gaaacaccgc aacccggact gacttataat
    17821 ctccgggtcg gaaccactcc cggcgggaac gagatcatgc cgtcgatgtc cgattccacc
    17881 ggctggcgta aggtggccca aatgggaaat gtttatcaga atacgacctg gtggcttcat
    17941 gatttgcagc cgggaaccta ttactggagc gtgcaggcga tcgataacag ctatgccggt
    18001 ggtccttttg cccaggaaca gttgttttca atttatgcgc tgtcgacaac ctggacaggc
    18061 gccatcgact ctttgtggag cagggcagat aactggagcg acggactccc gggtgaaaac
    18121 accacagtaa ttattcctgg agaaccggtg aatcaacccg tgctgaacat ttcgaccgcg
    18181 aacaaaaaat tgatccttca aacagggagt gaattgatta tcaattcttc caaactactg
    18241 aacctttccg atagcctgat cattaatcag cattcaccag cagattcgac aaaattgata
    18301 atccggggga atattgatat tatccgggaa tgatttaatg acactaacta aaagaaccct
    18361 tgtctaacta tctgtcaaac aagggttttg gatttgattc cgaggtctcg agcgaaggtc
    18421 aaagtcttac ttttacttaa tttctcttaa tctaaaaagt aaatatttgc actgatattc
    18481 agatatttaa agttattttc tttatctttg aggtaaagaa aaataaagtt agagaaggtc
    18541 cagaccgccc ctgtgaccgc ccccagtttc aggaatctca atgataatgc acaaacagga
    18601 atcccatggc aaagattaaa cctcaattca agctgaaatc aagcaaacca gtggatgtgg
    18661 ccacactgat ttacttgatc atttattaca acaataaaag gttcaagtgc tcgaccggag
    18721 aaaagattga gccaaggtat tgggactctg tttctcaaag agccatgatg ggtaccggtg
    18781 aaaagaccag ggaccaggac catgcagata tcaattttca gcttaataag catcataacc
    18841 aggcaatcat aatctttgac cgatttgaga gggaggggaa aataccatca ttaaaaacag
    18901 ttaagcaaga gcttgataaa gggttgtatg tcgaagaaaa gaaagagcct aaaggccgga
    18961 tttctcttaa caagtatatc gatcagttta taaaagacat tgagaaagga aagcgactca
    19021 ctgataaagg actgaagtac agtcatggga ccatcaaaaa ttacaaagga ttccaaaccc
    19081 agttcgatga attccagcaa gaaacacacc gta
//
LOCUS       DAOPNH010000021        18956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2797_length_18956_cov_7.204169, whole genome shotgun sequence.
ACCESSION   DAOPNH010000021 DAOPNH010000000
VERSION     DAOPNH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18956
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_18956_cov_7.204169"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..3384
                     /locus_tag="PKZ74_02210"
     CDS             220..3384
                     /locus_tag="PKZ74_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPM91593.1"
                     /translation="MKKVFSVLLLLTTIASTAQVTTLDEFENSSGWSFNKSDGVNVNL
                     VNEPGLTGDAIRFDYDFTKGTGYGGIQKLFPIDLPENFEFTFWLKADSPANNFEIKFI
                     DSTGNNVWWVNNRNYDFPNEWKKIRIKKRHISFAWGPTADQSLKRIDRIEFTIASYVG
                     GKGTIRLDQLDFEPLPPETSLYPIPEITASSVLKSHTTDRMTDNLTETGWQSTGSKGQ
                     YVLVDFKTRREFGGLQIDWLKGHYAVDFDILLSMDGIEWEKAYQVQSNRGGTSFIRLP
                     EAEAQFLKIDLKKSIMEKWFGIAEIKFLTIKNSLTTNDFLIYTAKNSPPGSYPRSFLE
                     QATYWTITGVNNDVKEAMISEDGMVEVDKGLFSIEPMIRTIDSLYNWRNVKSSQVMGF
                     PGDSGFSFVPSVTWDFDEIHFTTGISAIGNANKDSRLVIRYLFENQSVTNKDFLFYLL
                     IRPFQVNPYYQWLNLTGGAGKISTIREADEGMIAVDGKMILPSIPYDAFSAVTFDQGD
                     ITEGLRKGILPGTAMADDASGLASGVMKYILHLNPGESKELAVVVPFYPEGIPAALSA
                     KESLDEDIGQMVQFWRERVDHIRFQLPSSADRIIDTYRANLAYILVNRDNAGIQPGSR
                     SYERSWIRDGALTSSALLKSGIVKEVREFIDWYSDHLFDNGKVPCVVDFRGPDPVPEN
                     DSHGEFIYLIREYYNFTHDTAFLRSQNQNILKVVDYIESLIAQRSTDYYRNGSDSLRA
                     HYGLVPESISHEGYSAKPMHSYWDNFFTIKGLKDAAEIQAILGESENYDRIAALRDTF
                     RKNLYNSIDLAMKYKGIDYIPGCVELGDFDATSTTIALTPCNELKNLPKPQVYNTFDK
                     YYRFFLDRREGRIDWINYTPYENRLIGSFILLDQPERAHELIDFFLNDQRPQGWHHWA
                     EVVWKDDRYPGYIGDMPHTWCGSDFLNAIRSLFVYENEYDQTLVLGAALYQEWIDSPD
                     GMSVENLPTYYGDISFSIKKEKNKYHFKISGDIDLPDNGMEIRNFNGSKKPKKVTVNG
                     IEIEEFSDKKIPVKVFPAEVLIYY"
     gene            3526..5310
                     /locus_tag="PKZ74_02215"
     CDS             3526..5310
                     /locus_tag="PKZ74_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM91594.1"
                     /translation="MEEQVKTAVHKTSPEDRIKFSQLAAYGAGGIIPIALFNIAGILV
                     GLMGNISLGLSAFWLGVILIIPRLWDAVSDPIIGHISDNTRTRFGRRRPFLLIGGILV
                     AVFFVVMWWIPKGETVHGWFPTEAGFQAFQLVYILIALLLFYTSTTIFEIPHGALGME
                     MTSDYHERTRLFSAKSFVGNLFAMSTPWLFALANMEIFRGVGGNEADGMRYVSLMIAA
                     LIIPLSFWWTYKLREPGFVRVVKQEKSPFWKDMKHAAGNRNFLMLTFTIFTLAMGFNF
                     VQLLGSYIPIFYVFGGDKVAGAYLLGINGTIWAVTGVLAVFPLNWISPKLGKRNTLVI
                     AILLMVLAQLSKIVCYNPDYPYLIIIPTMLLSAGMLFFFTLGSSMVGDICDEDDLKTG
                     KRTEGSFYSIFWWFIKMGTALASFVAGLLIVLTMFDETQVTKVDALQGSIRELRKEVQ
                     ANTGKGSQEVSLSDFLQKAQTKSSELMKHLEEKAASDKHNKEHFDALLQNMSGVNQRI
                     AGLDLSSSKESLDNEISGIENDITQLTIQSPFTLLMMRIVEIGLPVLLSLLSIFFVLR
                     YSLTEKRSHEIKELLKKRNEEISMGTND"
     gene            5386..7821
                     /locus_tag="PKZ74_02220"
     CDS             5386..7821
                     /locus_tag="PKZ74_02220"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPM91595.1"
                     /translation="MNFEIKQSHFYLNNKKIFLNSGEIHYFRIKRELWDIHLDAAVEA
                     GLTTVSTYVPWAWHEPEEGQFDFTGKTTPERDLQGWLQRCKAHGLTCILKPGPFILAE
                     TRGAGLPGWFLQKHGREVKMRNSRGEEVASDGVNLFHPVYLEKVGRWYDRIMPFIRDH
                     EMENGGPVIMMQICNEIGVFSWLAHQGDYGEGVKERFGNYLMRKFGNVGEVNRLWGKN
                     YTGFKDVELPPDGREPYVSKGDRGRDYEWHCFWRTYYGDYLRLLTMMARDRGVTVPFY
                     HNLPGWIYGSGYEFPLNITMYDDLFGDRSEILFGVDHIPEYLSHRNQHDDRIINDITA
                     AMQGDKPLFAAEFQCGSREYHVVTNPREMELFYKASLAHGLNGWNFYMFSQGRNPRHR
                     GYSGSTFYWYTPLTPEAGRNTIFPLVGRMSRIVKTMETVIVEAERKAEIGVLFYPPYY
                     ATELERPETGASELAFNASAIRRPAYFDGLLRVLQVMNIDYDMADLTRSTAGSLSRYK
                     QVWAFATDEMNAADQQTLVDYATNGGNLVICPYLPDREMSQKPCTLLRDSISISPAGT
                     ETIDSPLIDLYGLQDIKCANPQAIYDEEKLAGCEIIARTIRGTACGFEKSFFGKGKII
                     HIGTWLGFDTEGHKPAYKAILERSGARLSRAFSDNENITVRQRFTPSGEGLLFIGNYY
                     NEEHTGKVTYSHPISGDSISIPFDGTTMQWPPLYAVLSPLCLELAKGIRILHCTSDIL
                     RITKSEVGIDLLLTGDRDLAGEIVFEGERVDSIKSVWIDREKMIMQQFSNRIVIKYRH
                     KQKEEFFLKLVFQ"
     gene            7933..11259
                     /locus_tag="PKZ74_02225"
     CDS             7933..11259
                     /locus_tag="PKZ74_02225"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPM91596.1"
                     /translation="MKIKSTNGLEIQFLENGAVKSIEAGPVRINLKPASFLSDPGTNL
                     FLRKRGNVPGFHPLTGPGSGSRFLVADGNYISTGSWEGLDYECRLVLDDEKMSWQWQV
                     EVKNNSGEPFEFDLVYLQDIGLKPVNAGLLNEYYVAQYLERLIFNDEDYGKVVICRQN
                     MKDATGHPWVMIACRNGAESACTDGMQFYGKTYRETGIPEGLISGSLGGEYAGESPVV
                     AVQERPFVLDPGETHRSVFLGIYLPDHPGASAAADLKMLRLPDEMGFRPKGTPSLGTP
                     SLFPLLPVTDLTETEIEYLFGKDRQHAEIVDDKLLSFFSGNNHIILKNKEILADRPHG
                     HIMQAKAGLVPDEEIMATTAFAFGVFNSHISQGNTNFNVLLSICTSQFNQARESGQRI
                     IVEIEGKKYLLGVPSAFEIGLNHCRWIYKFNDCIYQVRTWTSKSAPQINMDFKIVDSE
                     TVKSRVRLWITHDFDPLNKWKIFQGVEDCLYFALPEEDTMIKNKFKDARYKIKIQDNT
                     RFRAGGAELLFPDQRFTGNNLFVLQTEPTGHLSMSVIGEVIKPNDGIVFANVDEQFAK
                     DCSDAWSEWNNLSRGLALISDQKDVAAIREILPWYGTNALTHYLTPYGLEQFGGAAWG
                     TRDVSQGPVDLLLNLGKYKEARQVLLTIFSNQNPDGGWPQWWMFDSYDNIRSDSSHDD
                     IYYWTIIALASYIKTTEDSRILLEELPYYHEDSEKTSEITPLNEHIERLIRMINSSFI
                     PGTSLVPFGGGDWNDSLQPVGKELARRMISSWTVEMNYQAFRQYQAVCRMTGQERMAD
                     RLDEICLKIRSDFNKYLVKDGIVAGYGLLEDDGTISVMLHPSDTRTGIRFSILPMERG
                     ILSGIFTREQAEHHLEIIEKHLKGPDGVRLMDRPLKYKGGIQEIFHRAESSTFFGREI
                     GLMYVHEHIRYAEALAKMGRADAFVVALRQAIPVDYRSIVPMGDIRQANCYYSSSDVH
                     LKTRYEADERYNEVIEGSFTLRGGWRVYSSGPGIYIGLVISRLLGIRIEWGNVIFDPV
                     LPFSMDGLSASMDFMGKKVTFNYSVKEENFGPKAIRINGQDISTFNEDNIFRQGGAVI
                     PVKILDEILTKDFNLIEVLL"
     gene            11359..12771
                     /locus_tag="PKZ74_02230"
     CDS             11359..12771
                     /locus_tag="PKZ74_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPM91597.1"
                     /translation="MRRHLIVIGLILLFSRDAIPGDLVPGDAIPSVGIIKNIPDSTLL
                     EIVQRQTFRFFWHGAHPVSGLALERSNTVMAVHYWDYINEAWDEPNFGKVPFGPDACA
                     IGGTGFGIMATIVAVERGWIERDTAVRRLVKIADFLANADCYHGIYPHFMDGNTGKTI
                     KFDRLDDAADLVETSYLLMGFLCAREYFNSTEPIEVYLRNRITKMWNAANWAWHTNGE
                     KKLYWHWSPNNGFDMNFPIWGWNECLITYIMAASSPFHAISRDVYEGTWTGSSGFKNG
                     KKYYGIELPLGNYEGGGPLFFEHYTFMGIDPNGLKDDMGIDYLEQVRNHTLINRAYCI
                     ENPKNYKGYGGNCWGLTAGDSYKGYVAHCPDSDFGVIQPTAALSSFPYTPEYSMHALK
                     HFYYDLGDKIWSEYGFTDGFSIHHDWYAESHLAIDQGPIVVMIENYRTGLLWKLFMQI
                     PDIQKGLETLGFESPWIKGK"
     gene            12898..15099
                     /locus_tag="PKZ74_02235"
     CDS             12898..15099
                     /locus_tag="PKZ74_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPM91598.1"
                     /translation="MIKLPSLLLKPLTTLTILTTFTTFTTLTTLTTFSQEYRYNYCFF
                     TNSPMPGDWYFSMSAAKEGSSVKNSSGKLPVNDQVFHSPGNSLELFCESEPGGNWNVL
                     LLHSGKRGMDFFKKSDALSIWIYNNSENTSENEVPYIQVMQYDSSLSSAFPLPLTPFR
                     IWQQVIIPFDSLGIKRSSRAETFPGLYLSQNPASSGSIELYLDDIELITTGAASAVVQ
                     LPEITETKGYARHVDIKWNEVTDPNVRLVKIYRQAEDTGPVPVGVQQPYINRYADFTG
                     ESGKKYSYTITFLDAGYHETGPSPAAKAETRALSDDELLTMVQEACFRYYWEGAEPNS
                     GMARENIPGRRNMIATGASGFGIMALLVGTERKFITRNESIERFLKILNFLEKADRFH
                     GAYGHFMNGTTGEVVPFFGQRDNGADLVESAFLLQGLLAARQYFDGKTADEQMIRDKI
                     TAIWEGLDWSWFRKEPDSRFLFWHWSPDRGWVINHRLIGWNETMVVYLLAIASPTHPV
                     PGSMYFTGFASREPLAMKYRYDWGGSMDGALYINGNHYSGIKLDVGVNNGGPLFFTHY
                     SYMGYDPHFITDQYTNYFNNNTSIAKINYNYCVSNPKRYKGYGENAWGLTACDGPAGY
                     SADEPIPSRDFGKLAPTGAIASFPYTPVESMKALKNYYLNLGHFLWGEYGFKDAFNLT
                     DNWCSEIYMGLNQAPMTVMIENYRTGLVWKLFMQDPEIREGLKRLSVLKSE"
     gene            complement(15170..16366)
                     /locus_tag="PKZ74_02240"
     CDS             complement(15170..16366)
                     /locus_tag="PKZ74_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPM91599.1"
                     /translation="MSEFSRNQASRADQLVGYVIGLIEGRKGSGLLEQYKVLETDFQP
                     GDILILFDNLFEKKYPMEGIKSASNKLFNILFKTLSPYRRNDYPRNSFLWILQKDNAG
                     IKKILGKARNDIKKINSDADLLALSRLEATFRGAEKFLEHYVVMENIVFPEIEKQWKN
                     HQCLKLMWSFHDDIRRNLHATCEILRAAPFNLKQFNETVSKVYFNISTIVFREEHVLF
                     PILHESFGDELFVKMGDQLREFDLAYADVRKIRASGQMPEERSLTGDMVRLATGELTP
                     DQIDLIFNHLPVDITFVDENNRVKYFSSPKHRIFPRTASVINRRVQDCHPHESVHIVN
                     KIVDSFRSGEKDEAAFWIRMKDKFVLIRYFAVRDKEGNYRGVLEVSQEISGIQAITGE
                     RRLLDW"
     gene            complement(16407..16745)
                     /locus_tag="PKZ74_02245"
     CDS             complement(16407..16745)
                     /locus_tag="PKZ74_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPM91600.1"
                     /translation="MEKIIAACGLNCAQCEARIATLADDNDLRAKTAEKWKVQFNAPD
                     IPIEAINCTGCMEPGVKLSHCSECKIRNCAIGKGFTTCAECSILESCELLKMVHQYDP
                     SALENLKMMN"
     gene            complement(16767..17246)
                     /locus_tag="PKZ74_02250"
     CDS             complement(16767..17246)
                     /locus_tag="PKZ74_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91601.1"
                     /translation="MLLNLIDILRIVIVCFGIFWGYTIGFDNGYDPVAQLHVTIPVVI
                     VAIAGFSGLEGLLFGAKTAENKGFKTDGNYQRQSAIALLSYAAVALVVYFCKWGIKAE
                     LTIFFAFIFFFIFSGINHAVDAFRNKNYKWQNINRPFITFFLTAALIYPIIKVIQDL"
     gene            complement(17283..17918)
                     /locus_tag="PKZ74_02255"
     CDS             complement(17283..17918)
                     /locus_tag="PKZ74_02255"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HPM91602.1"
                     /translation="MKPSPREKSSMIFRSALGAMAWIAVILDFILSDELIWNFISYFT
                     IISNTLIAVAMTLTVTVPGSRFGAFFSRSGIQSALALYIIIVGLVYNFNLRNTWAITG
                     FLSIVDYLLHVVIPLAYIVYWLIFIPKRALNWTHSIIWLIFPLVYLGYSLARGAITGW
                     YPYPFLDVAKLGYEKVLLNSAFVTSGFLLFAVLLIFINRSVKLLPKRTRST"
     gene            complement(17915..18349)
                     /locus_tag="PKZ74_02260"
     CDS             complement(17915..18349)
                     /locus_tag="PKZ74_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM91603.1"
                     /translation="MADKNTALTASIKLINNKLHFEGHAGDNAPVSIDYIAPLGDDLG
                     YTSLELLLLSYSSCLGSAVLTFLRRMGKTIRGFEIQAKGIQHTEHPTGFSTIHIEMEL
                     GSPDVLPEDLEKVIALAKEKYCPVWAMIKGNIDIIINYSIHP"
     gene            complement(18458..18868)
                     /gene="rnk"
                     /locus_tag="PKZ74_02265"
     CDS             complement(18458..18868)
                     /gene="rnk"
                     /locus_tag="PKZ74_02265"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPM91604.1"
                     /translation="MSKLIINKLDYARIRKSIEDARQFKSISSIDAEKLMNELNSAEI
                     VEPEAIPSNVVTMNSIVKISFLNNDKQIQFQIVYPSKANIKENKISIFSPIATALIGY
                     KVKDEIEWIVPAGLTKIRIDEIIYQPEAAGEYHL"
BASE COUNT         4978 a         4670 c         4720 g         4588 t
ORIGIN      
        1 aggctagggt ttgaatattt gaaatagaga tattttttaa tgattaacca caaaggaaca
       61 caaagtattt cacaaaggcg cacaaaggtt ttaagctctc aatttacagt ttacggttta
      121 caatctatga tctgaatttg taatttataa ttttttaagc cttggtgttt cgttgtgaat
      181 tcctttgtgc gcctttgtgg taaaaaaagt cttaataaaa tgaaaaaagt gttttcggtc
      241 ctgctgcttc ttacaacgat tgcatcgacc gcccaggtta ctaccctgga tgagtttgaa
      301 aatagcagcg gttggagctt taacaaatca gatggggtta atgtaaacct cgttaacgaa
      361 ccgggcctga ccggcgatgc catccggttc gactacgatt ttaccaaagg gacaggttat
      421 ggaggcatac agaaactctt cccgatcgac ctgccagaaa attttgaatt caccttctgg
      481 ctgaaagcgg attctccggc caataatttc gaaatcaagt tcatcgacag caccggcaat
      541 aatgtctggt gggtgaacaa tcgcaattac gatttcccga atgaatggaa aaagatcagg
      601 atcaagaaac ggcacatctc ctttgcctgg ggtccgacgg ccgatcaaag cctgaagagg
      661 attgaccgga tcgaattcac tatcgcatcc tatgtcggag gcaaaggtac gatccggctc
      721 gatcaactgg attttgaacc gctgcctccc gagaccagcc tgtacccaat tcctgagatt
      781 acggcctcct ctgtgctgaa aagccacacc accgaccgga tgaccgacaa tttgaccgaa
      841 accggctggc aaagcaccgg cagcaaaggg caatatgtgc tggtcgactt taaaacgcga
      901 agggaattcg gcggactgca gatcgattgg ctgaaagggc actatgccgt ggattttgat
      961 atcctgttgt cgatggatgg aatagaatgg gaaaaggctt accaggtgca gtcgaaccgg
     1021 ggggggacga gttttatccg cctgccagag gcagaagcac aatttcttaa aatagacctg
     1081 aaaaaaagta ttatggagaa atggttcggt attgcggaaa tcaaattcct gaccatcaaa
     1141 aactctttaa caaccaacga tttcctgatt tataccgcaa aaaactctcc gccgggaagt
     1201 tatccccgtt ctttcctcga acaggccacc tactggacga tcaccggcgt aaataacgat
     1261 gtgaaagagg ccatgatcag tgaagacggt atggtggagg tggacaaggg gctcttttcc
     1321 atcgaaccga tgatcagaac aatcgactcg ctctacaact ggagaaatgt gaaatcaagc
     1381 caggtgatgg gcttcccggg tgattcgggc ttttcatttg tcccttccgt cacctgggat
     1441 tttgatgaaa tccatttcac gacgggaatt tcagccattg gaaacgccaa taaagattcg
     1501 cgtctggtta tccgctatct ttttgagaat cagtccgtaa ccaacaaaga ttttcttttc
     1561 tacctgctga tccgtccgtt tcaggtcaat ccctactatc aatggctgaa cctgacggga
     1621 ggcgccggga aaataagcac gatcagggag gcggatgaag ggatgatcgc ggttgacggc
     1681 aagatgatcc ttccgtccat cccctatgat gcttttagcg ccgtaacctt tgaccagggc
     1741 gatattaccg aaggattacg gaaagggata ctgcccggca cggcaatggc agatgatgcc
     1801 tccggcctgg cttccggcgt gatgaaatat atcctgcacc tgaatcccgg cgaatcgaaa
     1861 gaattggccg ttgtggtacc gttctatccg gaagggatcc ctgctgccct gagcgcaaaa
     1921 gaaagcctgg atgaggatat tgggcaaatg gtccaattct ggcgggaacg ggtcgatcac
     1981 atccggttcc aactgccgtc gtcagccgac cggatcatcg atacctacag ggccaatctt
     2041 gcctatatcc ttgtcaaccg cgacaatgca gggatacaac ccggatcgag gtcgtatgaa
     2101 aggagctgga tcagggatgg cgcactgacc tcctcggcct tgcttaagtc gggaattgta
     2161 aaagaggtca gggaattcat cgactggtat tcggatcatc tgttcgacaa cggcaaagtc
     2221 ccctgtgtgg tcgatttccg cggtcccgat ccggtacctg aaaacgacag ccacggcgaa
     2281 ttcatctatc tcatccgcga atattacaat ttcacgcacg acacggcatt tctcaggtcg
     2341 cagaatcaaa atatcctgaa ggttgtggac tacatcgaat cgctgattgc ccagcggtcg
     2401 accgactatt acagaaacgg cagcgacagc ctcagggcgc actacgggct ggtccccgaa
     2461 tcgatcagcc atgaagggta ttcggctaaa cccatgcatt catactggga caattttttc
     2521 acgatcaaag gtttgaaaga tgccgctgag atccaggcca tcctgggaga atcagaaaat
     2581 tacgacagga tagccgcact ccgcgacact ttccggaaaa acctgtacaa ttctattgac
     2641 ctggccatga agtacaaagg gatcgattat atcccgggct gcgtggaatt gggcgatttc
     2701 gatgcgacat caacaaccat agcgctgacc ccgtgcaacg agctgaaaaa tcttccaaaa
     2761 ccacaggtat acaacacatt tgacaaatac taccggttct tcctcgaccg acgtgaaggc
     2821 agaatcgact ggatcaatta cactccttat gaaaaccggc tgatcggctc ctttattctc
     2881 cttgaccagc ctgaaagggc ccacgagctg atcgatttct tcctgaacga ccagcgtccg
     2941 cagggatggc accactgggc cgaagtggtc tggaaggatg accggtatcc cgggtacatc
     3001 ggcgacatgc cccatacctg gtgcggcagc gattttctca acgctattcg ctcccttttc
     3061 gtctatgaaa acgaatatga tcaaacactt gttcttggcg ctgcccttta ccaggaatgg
     3121 atcgattcac ccgatggcat gtcggtggaa aatttaccga cttactacgg agatatctct
     3181 ttttccatca aaaaagaaaa aaataaatat cattttaaga tttccggtga tatagatttg
     3241 ccggataacg gcatggagat caggaatttc aatgggtcaa agaagccgaa gaaagttacg
     3301 gtgaacggga ttgagatcga ggaattttcc gataagaaga ttcccgttaa ggtattcccc
     3361 gctgaggtgt tgatttacta ttaaagtggt taattttaca tagattgtca acaaagtgaa
     3421 ccgcaaaggt gcacaaagga tatcacaaat gcgcacaaag taaataatta ccaataaaaa
     3481 tccttcgcgt tcctttgtgt tactttgtgg ttaaaaaaat caaatatgga agagcaggta
     3541 aaaacggctg tgcacaagac cagccctgaa gacaggatta aattctcgca gttggcggcc
     3601 tacggggcag gaggtatcat ccccatcgcc ctgttcaaca ttgccgggat actggtcgga
     3661 ttgatgggca acatcagtct gggcctgagc gctttctggc tcggggtgat cctgatcata
     3721 ccccgtttat gggatgccgt ttccgatccc atcatcggtc acatctcgga caatacgcgt
     3781 acacgattcg ggcggcgccg tcccttcctg ctgatcggag ggattctcgt cgccgtgttt
     3841 ttcgtggtga tgtggtggat cccgaaaggt gaaacggtac acggctggtt ccccactgag
     3901 gccggtttcc aggcttttca gctcgtctat atcctgatcg cgcttctcct tttctatact
     3961 tccactacca tttttgagat acctcacggt gcactgggca tggagatgac ctccgattac
     4021 catgaaagaa cccggttgtt cagcgccaaa agctttgtcg gtaacctgtt tgccatgagc
     4081 actccctggc tttttgccct cgccaatatg gaaatcttcc ggggggtcgg aggtaatgaa
     4141 gccgatggca tgcgatatgt ttccctgatg atcgctgcac tgatcatccc cctctcattc
     4201 tggtggactt ataaactccg tgaaccgggc tttgtaaggg tggtaaagca ggaaaagtca
     4261 cctttctgga aagatatgaa acacgctgcc ggcaaccgga attttctcat gctgaccttc
     4321 actattttca ccctggcgat gggttttaat ttcgtacagc ttctcggatc gtatattccg
     4381 atcttctatg tctttggcgg tgataaagtg gccggcgctt acctgctggg gatcaacggg
     4441 accatctggg ctgtgaccgg cgttttggcc gtgtttcccc tgaactggat cagtccgaaa
     4501 ctgggtaaaa gaaacacgct ggtgatcgcc atcctgctca tggtgctggc gcagctttca
     4561 aaaattgtct gctacaaccc agattatcct taccttatca ttatcccgac catgctctta
     4621 tccgccggaa tgctgttctt ctttaccctg gggtcttcca tggtgggcga tatttgtgat
     4681 gaagatgatt tgaaaaccgg caaacggaca gaggggagtt tttattcgat tttctggtgg
     4741 tttatcaaga tggggactgc cctggccagc tttgtagccg gtttgcttat cgttttaacg
     4801 atgttcgatg agacgcaggt tacaaaagtc gatgccctcc agggaagcat cagggagctc
     4861 cggaaagagg tccaggcaaa taccggcaaa ggaagtcagg aagtttccct gtcggacttt
     4921 ctgcagaaag cacagacaaa atcctccgaa ctgatgaaac acctggaaga aaaagcggcg
     4981 tcggataaac acaataagga acacttcgat gcactccttc aaaatatgtc tggggtcaac
     5041 cagcggatcg ccggcctgga tctgtcatct tccaaagaat cacttgataa tgagatcagt
     5101 ggcatcgaga acgacatcac acaactgacc attcaatcgc cttttacgct cctgatgatg
     5161 agaatcgtgg aaatcggatt gccggtactg ctcagcctgc tgtcgatttt ctttgttctc
     5221 cgttacagtt taacagagaa acgttcgcat gagataaaag aattattaaa aaaaaggaat
     5281 gaggaaataa gtatgggaac gaacgactaa agcggttggt tccttactgc gaacaataaa
     5341 ccgacggcta tagtcgtcgt tgggtagaaa atacaaatag ctgtcatgaa ctttgaaata
     5401 aagcaaagcc acttttatct caacaataaa aagattttcc tgaactcagg ggagatacac
     5461 tatttcagga ttaaacggga actgtgggac atacatctcg acgccgctgt tgaggccgga
     5521 ctgaccaccg tcagcaccta cgttccctgg gcctggcatg aacccgaaga agggcagttc
     5581 gatttcaccg gaaaaaccac gccggaacgc gatctgcagg gctggttgca gcgctgcaaa
     5641 gcgcatggcc ttacctgcat cctgaagccc ggccccttta tcctggccga aacccggggt
     5701 gccggcctgc ccggatggtt cctgcaaaag catggccgcg aagtgaaaat gcgcaacagc
     5761 cggggcgaag aggtggccag cgacggcgta aacctgtttc atccggtgta cctggagaaa
     5821 gtcggccgct ggtacgaccg tatcatgccc tttatccgcg atcatgaaat ggaaaacggc
     5881 ggcccggtga tcatgatgca gatatgtaat gagatcggag tcttttcgtg gctggcacac
     5941 cagggagact atggcgaggg ggtgaaagag agatttggaa attatttgat gagaaaattt
     6001 ggaaatgtgg gggaggtcaa tcgcttatgg ggtaagaatt atactgggtt taaagatgtt
     6061 gagctgccgc cggatggtcg ggaaccctat gtttcgaaag gtgaccgcgg aagggactac
     6121 gaatggcatt gcttctggcg gacctattac ggcgattatt tgcggctgct gacaatgatg
     6181 gcgcgcgaca gaggcgtcac cgttcccttc taccacaacc ttcccggctg gatctacggc
     6241 agcggctatg agtttccgct gaatatcacc atgtacgacg atctgttcgg tgacagaagc
     6301 gagatcctgt tcggcgtcga tcatattccg gagtatcttt cgcaccgcaa ccagcacgac
     6361 gaccgcatca tcaacgacat cacggcggcc atgcaggggg ataaaccgct gtttgccgcc
     6421 gaattccagt gcggttcaag ggaatatcat gtggtcacga atccgcggga gatggagctg
     6481 ttttacaaag ccagcctggc gcacggcctt aatggctgga atttttacat gttctcccaa
     6541 gggcggaatc cccggcaccg ggggtattcc ggctccactt tctactggta caccccattg
     6601 acaccggaag ccgggcgaaa cacgatcttc ccgctggtcg gccgaatgag ccgcatagta
     6661 aaaacgatgg agacggttat cgtcgaagcc gaaagaaaag cagagatcgg cgtgctgttt
     6721 tacccgccat actatgccac ggagctggag cggcccgaga caggcgcatc ggagctggca
     6781 ttcaacgcct ccgccatccg ccggcccgca tatttcgacg ggctgctgcg cgtactccag
     6841 gtcatgaaca tcgattacga tatggccgac ctgacccggt cgaccgccgg atcgctcagc
     6901 cggtacaagc aggtgtgggc ctttgccacc gacgagatga atgccgccga tcagcaaacc
     6961 cttgtcgatt atgcgacgaa cgggggcaac ctggtcatct gtccatacct gcctgaccgg
     7021 gagatgtcgc aaaaaccctg caccctgctt cgagactcca tctcaatttc acccgccggc
     7081 acagagacca tcgactcccc gctgatcgac ctctatggcc tgcaggatat caaatgcgcc
     7141 aatccacaag cgatctatga cgaagaaaag ctggccggtt gcgaaatcat cgcacggacc
     7201 atccggggaa cggcctgtgg gtttgagaaa tctttttttg gaaaaggaaa aatcattcac
     7261 atcggtacct ggctgggctt tgataccgaa gggcacaaac cggcctataa agccattctt
     7321 gaaagatcgg gcgcccggtt aagcagagca ttttctgata atgagaacat taccgtaagg
     7381 caaagattca ccccgagcgg cgaagggttg ctcttcatcg gaaattatta taatgaagaa
     7441 cataccggca aggtgacgta cagtcatccc atctcaggag actccatctc gattccattc
     7501 gacggcacga cgatgcagtg gcccccgctt tatgcagtgc ttagcccgct ctgtttggaa
     7561 cttgcaaagg gaatccggat ccttcattgc acctctgata ttctccggat cacaaaaagc
     7621 gaggtaggca ttgatcttct actgaccggt gaccgcgacc tggctgggga gatcgtattt
     7681 gagggagaga gggttgacag tataaaatca gtgtggattg atcgagaaaa gatgataatg
     7741 caacagttta gcaatcggat tgtcatcaaa tacaggcata agcaaaagga agaatttttt
     7801 ctgaaacttg tttttcagta gattgataat ttttttaaac cacagattac atagagtaag
     7861 aggctgtctc atttgaaacc tcagtgtcct ctgcgtattt tctgtggtct atgtgattaa
     7921 aatacttcaa aaatgaaaat taaaagcacc aacgggctgg agattcaatt ccttgaaaat
     7981 ggggctgtta aaagtataga agcgggcccg gtacggataa acctgaaacc ggcgtcattt
     8041 ctctctgatc ccggaacgaa cctgttcctc aggaaaagag gtaatgtgcc gggttttcat
     8101 cctttaacgg gtccgggaag cggcagccgt ttcctcgtcg ctgacggcaa ctatatctca
     8161 acaggtagct gggaaggtct tgattatgaa tgcaggctcg tcctggacga cgagaagatg
     8221 agctggcaat ggcaggtgga agtaaagaac aactccggcg aacctttcga gttcgacctg
     8281 gtttatctcc aggatatcgg gctgaagcct gtgaatgcgg gattactgaa cgaatattat
     8341 gttgcccagt atcttgagag gctgatattc aatgacgaag attacggtaa agtggtgatc
     8401 tgccggcaga atatgaaaga tgcgaccggt catccctggg tgatgatagc ctgcaggaac
     8461 ggagcggagt cagcctgcac cgacggaatg caattttacg gaaagacata ccgtgaaacg
     8521 gggatcccgg aagggttgat atcaggaagt cttggcgggg aatatgcagg agaatcgccg
     8581 gtggtggcgg tgcaggaaag acctttcgtg cttgacccgg gtgaaaccca tcgaagtgtt
     8641 ttcctgggga tctatttgcc cgatcatccc ggcgctagtg ctgctgctga tttgaagatg
     8701 ttgaggctgc cggatgagat gggatttcgt cccaaaggga cgccatccct tgggacgcca
     8761 tcccttttcc cattactccc cgttactgac ctcaccgaaa cggaaattga atacctgttc
     8821 ggtaaagatc gacaacatgc agaaattgtt gacgataaac tactctcgtt cttcagcgga
     8881 aacaaccata tcatcctgaa aaacaaagaa atacttgccg acaggcccca tggtcacatc
     8941 atgcaggcca aagcaggact tgttcctgac gaagaaataa tggcaacgac tgcctttgct
     9001 tttggcgttt ttaattcaca tatatcgcag ggcaatacca acttcaatgt cctcctgtca
     9061 atctgcacca gccagttcaa ccaggccagg gaatcgggcc agcggatcat tgtagaaatt
     9121 gaaggtaaaa aatacctcct gggcgttcca tctgcatttg aaataggtct gaaccactgc
     9181 cgctggatct acaaattcaa cgattgtatc taccaggtcc gcacctggac gtcgaagtct
     9241 gctccgcaga tcaatatgga ttttaaaatt gttgattcgg aaaccgtaaa atcaagagtg
     9301 cgattgtgga taacccatga ttttgatccg ctgaataagt ggaaaatctt tcagggggtg
     9361 gaagactgcc tgtattttgc ccttccagaa gaggatacca tgatcaaaaa caaatttaaa
     9421 gacgcccgtt acaagataaa gatccaggat aataccagat tccgggccgg cggtgcagag
     9481 ctattattcc cggatcaacg ttttaccggt aacaatttat ttgtccttca aaccgaaccg
     9541 accggtcatc taagcatgag cgttatcggg gaagtcatca aaccaaatga tggaattgtc
     9601 tttgccaatg tcgacgagca gtttgcaaaa gattgctcag atgcgtggtc ggaatggaac
     9661 aatctcagtc gggggttggc tctcatcagc gatcagaaag acgttgcagc catccgggag
     9721 atccttccct ggtatggaac gaacgccctc acacattacc ttacgccata cgggctggag
     9781 caattcggtg gcgctgcctg gggaacccgc gatgtatcgc agggaccggt cgacctgctc
     9841 ctgaacctcg gaaaatacaa ggaggcccgg caggtcttgc tgaccatctt ctccaaccag
     9901 aatcccgacg ggggctggcc ccaatggtgg atgttcgaca gctacgacaa tattcgttcc
     9961 gacagttccc acgacgatat ttattactgg accataatcg cactggcttc atatataaag
    10021 actacggaag attcaagaat attattggaa gagcttccct attatcatga agacagcgaa
    10081 aaaacttctg aaattacgcc tctgaatgag catatcgaaa ggctgatccg catgataaac
    10141 agctctttta ttccaggcac ttcgctcgta cctttcggtg gcggcgactg gaacgattcg
    10201 ctgcagccgg ttggcaaaga gctggcccgg cgcatgatct cctcctggac cgtggaaatg
    10261 aactaccagg ccttcagaca gtaccaggcg gtttgccgga tgaccggtca ggaaagaatg
    10321 gcagaccggc tcgatgaaat ctgcctgaag ataaggtcgg atttcaacaa gtacctggtg
    10381 aaagacggca tagtggccgg gtatggcctc ctggaagatg acgggacgat cagtgttatg
    10441 ctgcatcctt ccgatacccg aacaggcatc aggttcagta tcctgcccat ggaaagaggc
    10501 atcctgagcg gaatctttac cagagagcag gcagaacatc acctggaaat catcgaaaag
    10561 catctcaaag gtcctgacgg ggtaaggttg atggaccgtc cgttaaaata caagggcggc
    10621 atccaggaga tcttccatcg ggctgaaagc agcactttct tcggccgcga gatcgggctg
    10681 atgtacgtac acgagcatat ccgctatgca gaagctttag cgaaaatggg aagagccgat
    10741 gcgttcgtcg ttgcattacg acaggccata cctgtcgact accggagtat tgtcccgatg
    10801 ggcgatatcc ggcaggcaaa ctgttactac agcagttccg atgtacattt aaaaacccgt
    10861 tatgaggcgg atgagaggta taatgaggtg attgaaggaa gtttcacgct gcgcggcggc
    10921 tggcgggtct actcgagcgg ccccgggatc tatatcgggc tggtcatctc aagattgctg
    10981 gggatcagga tcgaatgggg caatgtgata ttcgatccgg tgctgccgtt ttcaatggac
    11041 gggctttccg cttcgatgga tttcatgggt aagaaagtga ctttcaacta ttcagtgaag
    11101 gaagaaaatt tcggcccgaa agctattcgg attaacgggc aggatatctc gactttcaat
    11161 gaggataata tctttaggca gggtggagcg gtcatcccgg tcaaaatact tgacgagatt
    11221 ctcacaaagg attttaattt aatagaagtg ctgctataaa tttgtcctga aaagattcgt
    11281 ggtttttaca actgtttatt agtggtttcg agaaaaaaat taattttaaa gaaacgaaac
    11341 ttgcaattct ttatgaagat gagaagacat ttgattgtaa tcgggttgat tttgcttttc
    11401 tcacgggacg ccatcccggg ggacctcgtc ccgggggacg ccatcccttc agtcggcatc
    11461 attaaaaaca tccccgacag cacactcctc gaaatcgtcc agcggcaaac tttccgcttc
    11521 ttctggcatg gcgcccaccc ggtcagcgga cttgctctcg aacgaagcaa tacggtgatg
    11581 gccgtccatt actgggatta tatcaacgaa gcctgggatg agcccaactt cggcaaagtt
    11641 ccatttggcc ccgacgcctg cgcaatcggt ggcacaggat tcgggatcat ggccaccatc
    11701 gtggccgtcg aacgaggatg gatcgaacgc gataccgccg tgcgaagact ggtgaaaata
    11761 gccgacttcc tcgcaaatgc cgactgctac catggcatct acccgcactt tatggacggc
    11821 aacaccggca aaaccatcaa attcgaccgc ctcgatgatg ccgccgacct ggtcgaaacg
    11881 tcttaccttc tcatgggatt cctctgtgcc agggaatatt ttaactccac cgaaccgatc
    11941 gaggtctacc tgcgcaaccg catcaccaaa atgtggaacg ccgccaactg ggcctggcat
    12001 accaatggcg aaaagaagct ctactggcac tggagcccta acaacggctt cgacatgaac
    12061 ttccccatct ggggatggaa cgaatgcctc atcacctata tcatggcagc ctcctcccct
    12121 ttccatgcca tctcccgcga cgtgtacgag ggcacctgga caggcagctc cggattcaaa
    12181 aacgggaaaa aatattatgg aatcgaactg cccctgggta attacgaagg cggcggaccc
    12241 ctgttcttcg aacattatac cttcatgggg atcgatccga acgggctgaa agacgatatg
    12301 ggcatcgact acctggaaca ggtacgcaac cataccctta tcaaccgggc ttactgcatc
    12361 gaaaatccaa aaaactacaa gggttacggc ggcaactgct gggggctcac ggcaggcgac
    12421 agttacaaag gatacgtagc ccattgtccc gatagcgact tcggcgttat tcagcccact
    12481 gctgcgctct catctttccc ttacaccccc gaatattcca tgcatgccct gaagcatttc
    12541 tattacgacc tcggcgataa gatctggagt gaatacggtt ttaccgatgg tttcagcatc
    12601 catcacgact ggtatgccga gagccatttg gccatcgacc agggcccgat cgtggtcatg
    12661 atcgaaaact accgcaccgg gctcctctgg aaactgttca tgcagattcc cgatattcag
    12721 aaagggttgg aaaccctcgg gtttgaaagc ccctggatca aggggaagta ggatccagag
    12781 ttcctggttc cgggttccaa tgttccagag ttccagggcc tgtcctgagg gcgaagcccg
    12841 aaggtttcct ggtttcttaa agttagatat tgtatttttg gctaataatc caatgttatg
    12901 ataaagctac cctctttact cctcaaaccc cttaccaccc ttaccatcct taccaccttt
    12961 accaccttta ccaccctcac aaccctcacc accttctccc aggaataccg ctacaactac
    13021 tgcttcttta ccaacagccc gatgcccggc gactggtatt tcagcatgag cgcggctaaa
    13081 gagggatcct ccgtgaaaaa cagctcggga aagctgcccg tgaacgacca ggtcttccat
    13141 tcaccgggaa actcactcga gttgttttgt gagagtgaac ccggtggaaa ctggaacgtc
    13201 ctcctgctcc attccggtaa aaggggaatg gattttttca agaaatctga cgctttatcc
    13261 atatggattt acaataattc tgaaaataca agtgaaaatg aagtgcccta tattcaagtc
    13321 atgcaatacg atagcagcct gagttctgct ttcccgcttc cgctgacgcc ttttcgcata
    13381 tggcaacagg ttatcatccc gttcgacagc ctgggcatta aaagatcgtc ccgggcagag
    13441 accttcccgg ggttgtacct ttcgcaaaat cctgcctcat ccggttcaat tgagctatat
    13501 ctcgacgaca tcgaactgat caccaccggc gccgccagcg ctgtggtaca attaccggaa
    13561 atcaccgaaa caaaaggcta tgcccggcat gtcgatatca aatggaacga ggttaccgat
    13621 ccgaacgttc ggttggtaaa aatttaccgg caggcggaag ataccggacc cgtgccggtt
    13681 ggtgtgcaac agccctatat caaccgttat gcagatttta cgggagaatc gggtaaaaag
    13741 tattcctata cgattacctt cctcgatgcc ggttaccatg aaacaggccc ctctcctgcc
    13801 gccaaagcgg agacccgggc cctgagcgac gacgaactgc tgacgatggt gcaggaagcc
    13861 tgtttccgct actattggga aggggccgaa ccgaacagcg gcatggcgcg cgaaaatatt
    13921 cccggacgcc gcaacatgat cgcaacgggc gcatccggat tcggtatcat ggctttgctg
    13981 gtcggaacag agcggaaatt catcacccgg aatgaaagta ttgagcggtt ccttaaaatc
    14041 ctcaatttcc tcgaaaaagc cgaccggttt cacggcgctt atggtcactt catgaacggc
    14101 acaacgggtg aagtggtccc cttcttcggt cagcgcgaca acggcgccga cctggtcgag
    14161 tcggccttcc tgctgcaggg attgctcgcg gcaaggcaat atttcgacgg aaagactgcc
    14221 gatgaacaaa tgatcaggga taaaatcacc gcaatctggg aaggccttga ctggagctgg
    14281 ttcaggaaag agcccgacag ccggtttctc ttctggcact ggtcgcccga caggggttgg
    14341 gtgatcaatc accggctgat cggctggaac gagacgatgg tggtttacct gctggccatc
    14401 gcttcgccaa cccacccggt gccaggcagt atgtatttca ccggtttcgc cagccgggag
    14461 cccttagcta tgaaataccg ctatgactgg ggtggttcca tggatggcgc actttatatc
    14521 aatggcaacc attactccgg aataaagctg gatgtcggcg taaataacgg cggcccgctc
    14581 ttcttcaccc actattccta catgggttac gatcctcatt tcattaccga tcaatacacc
    14641 aactacttca ataacaatac atccatagca aaaatcaatt acaattattg cgtttcaaat
    14701 cctaaacgtt acaaaggata tggcgagaat gcctggggac tgacggcctg cgacggcccg
    14761 gccgggtatt ccgccgatga gcccatcccg tcgagggatt tcggaaagct tgcccccacc
    14821 ggcgccatcg cttcattccc ttatacgccc gttgaatcga tgaaagccct gaaaaattac
    14881 tatctcaacc tggggcattt tttatggggc gaatatggct ttaaagatgc cttcaacctg
    14941 accgacaact ggtgttccga aatttatatg ggactgaacc aggcgccaat gacggtaatg
    15001 atcgaaaact accgcaccgg gctggtatgg aaacttttca tgcaggatcc ggagatccgg
    15061 gaagggctga agaggctgtc ggtactgaaa agcgaatgac attcattgat ggatgacggt
    15121 agtatttcaa acccggttga cgcatgtcat cccgatgcga agtaagtgat caccagtcga
    15181 gcagccggcg ttcgcctgta atggcctgga tacctgatat ttcctgcgag acttccagca
    15241 caccccgata gttcccctct ttatccctga ccgcaaaata ccggatcagt acgaacttat
    15301 ccttcattcg gatccagaaa gccgcttcgt ccttttcacc cgaccggaag gaatcgacaa
    15361 tcttatttac gatgtggacg ctttcatgcg gatgacaatc ctgcacgcgg cggttgatca
    15421 cgcttgcggt acggggaaag atccggtgct tcgggctgga gaagtacttc acccggttgt
    15481 tctcatccac gaaggtgata tccaccggca aatgattaaa gatcagatcg atctggtccg
    15541 gagttaattc gcctgtcgcc agccggacca tatcaccggt aagtgatctc tcctccggca
    15601 tctgcccgga agccctgatt ttcctgacat cggcgtaagc caaatcaaac tcccggagct
    15661 gatcccccat cttaacaaat aattcatcac cgaaagattc gtgaaggatt ggaaagagaa
    15721 cgtgctcctc cctgaaaacg atcgtgctga tgttgaaata gactttgctg accgtttcgt
    15781 tgaactgctt caggttgaaa ggggccgccc ggaggatttc gcaggtcgca tgcaggttgc
    15841 gccggatatc gtcgtgaaac gaccacataa gctttaagca ctggtgattc ttccattgtt
    15901 tttcaatttc cgggaacacg atattctcca tcaccacata gtgctccagg aacttttcgg
    15961 caccacggaa tgtcgcttcc aggcgtgaaa gtgcaagcag atccgcgtcg ctgttgatct
    16021 tcttaatgtc attccgcgct ttgcccagta ttttttttat accggcattg tccttttgaa
    16081 gaatccagag gaaggaattg cgcgggtaat cattccgacg ataaggcgaa agtgttttaa
    16141 acagaatatt gaaaagtttg tttgaagccg acttgatccc ttccatcgga tatttctttt
    16201 caaaaagatt atcgaaaaga ataaggatat caccgggctg gaaatccgtt tccagaactt
    16261 tgtactgctc cagcaaaccg ctccctttcc tgccttcgat caggccgatg acatagccta
    16321 ctagctgatc cgcacggctg gcttgattcc gggagaattc cgacatgtta aatcaggttc
    16381 agatttatcc aattggagac tccatctcaa ttcatcatct tcagattttc caaagccgaa
    16441 ggatcatatt gatggaccat tttcagcagc tcgcagcttt ccagtatact gcactcggca
    16501 caggtcgtga aacccttgcc aatggcgcag ttcctgattt tgcactcgga acaatggctc
    16561 agtttaacgc cgggctccat gcatccggtg caattgatcg cctcaatcgg gatatcgggc
    16621 gcattgaact gcaccttcca tttttcggct gtttttgcac gtaagtcgtt atcatcggcc
    16681 agggtagcga tcctggcctc acactgagcg caattcagcc cgcaggcagc gatgatcttt
    16741 tccataaact gttatttttt aatgttctat aaatcctgaa taacttttat tatcggataa
    16801 atcaatgcag cggtcagaaa aaaagtgatg aacggacggt tgatattctg ccatttgtaa
    16861 tttttgttcc ggaaagcatc cacggcatgg ttgatcccag aaaagatgaa aaagaagata
    16921 aacgcaaaaa agatcgtcag ttcggctttg atcccccatt tgcagaaata tacaacaagt
    16981 gcaaccgccg cataggaaag cagggcaatg gccgactgcc gctggtaatt tccatcggtc
    17041 ttaaagccct tgttctcagc tgttttcgct ccgaacagaa gaccttccaa accggaaaag
    17101 ccggcaatgg ccacgataac caccggaata gtaacatgca actgggcaac cgggtcatat
    17161 ccattgtcga acccgatggt atagccccag aaaataccga agcagacgat gacaatcctg
    17221 agaatatcga ttaagtttag cagcataggc gtaaagattc ttttttaaaa gccgatataa
    17281 aattatgtcg atcgtgtcct ttttggtaac agtttaacac tcctgttgat aaaaataagc
    17341 aataccgcaa acaggaggaa cccggatgta acgaaagcgc tgttaagcag cactttttca
    17401 tagcccagtt tagccacatc caggaaagga tagggatacc atccggtgat ggcgccgcgt
    17461 gccagcgaat aacccaggta aaccagcggg aagatcagcc agatgatcga atgggtccag
    17521 tttaaggccc ttttcgggat aaatatcagc cagtagacaa tataagccag cggaatgacc
    17581 acatggagca ggtaatcgac gatgctgaga aacccggtaa tggcccacgt atttcgcagg
    17641 ttgaagttat agaccagccc gacgatgatg atatataacg ccagggccga ctggatgccg
    17701 gaacgggaga agaatgcgcc gaagcgggat ccgggtaccg tcaccgtcag tgtcatcgct
    17761 acggcgataa gggtattgga gatgatggta aaatagctga taaagttcca gatcagctca
    17821 tccgacagga taaaatcgag tatgacggca atccaggcca ttgcgccgag cgctgaccgg
    17881 aatatcatgg aagatttttc ccggggcgat ggtttcatgg atggattgaa taattgatga
    17941 tgatatcgat gttccctttg atcatcgccc acaccgggca atatttttct ttcgccaggg
    18001 cgatcacttt ctccaggtct tcaggcaaaa catcgggcga tcccagttcc atttcaatat
    18061 gaatagttga aaatccggtg gggtgttcgg tgtgctggat gcctttggcc tgaatctcaa
    18121 atccccggat cgttttcccc atcctgcgca ggaatgtcag cacagcgctt ccgaggcagg
    18181 aggagtagct gagaagcagc aattccagcg aggtatagcc cagatcatcc cccagcgggg
    18241 cgatgtaatc gatggagacg ggtgcgttgt cgccggcatg tccttcaaaa tgcagtttgt
    18301 tgttgatcag tttgatcgaa gcggtgagcg ctgtgttttt atcagccata tcagtcaggt
    18361 aataatcggc aacaaatcta aaaataatat tccggaaaaa ggagactcca tctcgattta
    18421 gtgagatgga gtctcaatat gcggtcagga cttctttcta taaatgatac tccccggctg
    18481 cctcgggttg gtaaatgatc tcgtcgatcc tgatcttggt cagaccggct ggaacgatcc
    18541 attcgatctc atctttgact ttatacccga tcagggccgt tgcaatgggt gaaaagatag
    18601 atatcttatt ctctttgata tttgctttac tgggataaac gatctggaac tggatctgtt
    18661 tatcattatt taaaaagctg atcttcacta tcgaattcat cgttaccaca ttggaaggaa
    18721 tggcttccgg ctccactatt tctgccgaat tcagctcatt catcagtttt tccgcgtcaa
    18781 tgctgcttat tgatttaaac tgccttgcat cctcgatgct cttcctgata cgggcataat
    18841 ccaacttatt gattatcaat ttactcatga ttattattca tttgattaat atggggataa
    18901 agatacggct aatttttgat atctggatct cacgaagaca agagcatacg actatt
//
LOCUS       DAOPNH010000022        18898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2809_length_18898_cov_6.723452, whole genome shotgun sequence.
ACCESSION   DAOPNH010000022 DAOPNH010000000
VERSION     DAOPNH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18898
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_18898_cov_6.723452"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKZ74_02270"
     CDS             <1..275
                     /locus_tag="PKZ74_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM91605.1"
                     /translation="LKAGAMPGSEDWGKEDPDYWGLLNAEMHGQQFYGNIETEPGNYM
                     AFYDNVFDVITKGAEQAVTPQEARNVIRILELAFESQRAQSLVKIA"
     gene            complement(321..2546)
                     /locus_tag="PKZ74_02275"
     CDS             complement(321..2546)
                     /locus_tag="PKZ74_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM91606.1"
                     /translation="MKTNKIGLLKKIWLKAIVLFVIIILIQIPAYTQQRGVKWAREGS
                     AYFKVEKNEIIRYTLPENTPEVIITKKQLTPEGSSSALKFNYFSFSDDEQKLLIFTNT
                     KRVWRLNTKGDYWVLDRTAGTFTQLGKSLPASSLMFAKFSPDGKSVAYVSGNNLYAED
                     LATHLIKPLTTDGTVTLINGTFDWVYEEEFFCRDGFRWSPDSKSIAYWQLDAGNIRKF
                     YMINNTDSIYSQVIPIEYPKVGEDPSSCKIGVVSLENGLTTWMDIPGDPVQHYIVRLE
                     FIPGTEKLLLQQLNRKQNHSKLFMADAKTGNTQLLNEETDEAWVELFQPGNPYTVDYT
                     NSFNFVDKDRSIIWSSEKDGWRHLYEVYLDGSPEKLITRGKYDVIDLKFIDPATHDIY
                     FMASPDNATQKYLYRVDADDPGKPELLSPEILKGTHDYSFSGDGKYAFHSFSNYYTQP
                     SKELIALPDHKPLFENESIVLNLDSLKETPKVEFFTIRTKDKVELDGWMAKPTDFDPK
                     KRYPVVFFVYTEPASTTVTDTYGISENGLYGGDMAEDGYIYISLDNRGTPAPKGREWR
                     KSIYRQIGQLNIRDQAMAAEEILKWDFIDPERVAVWGWSGGGSATLNLMFQYPQIYKT
                     GIAIASVSSLLTYDNIYQERYMGLPQENPEDFENGSPLKYARNLEGNLLIIHGTGDDN
                     VHYQNAEMLLNELVKHNKQFQFMPYPNRTHGIYEGEGTLLHLSTLYTDFLKSNCPPGG
                     R"
     gene            2756..4228
                     /locus_tag="PKZ74_02280"
     CDS             2756..4228
                     /locus_tag="PKZ74_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM91607.1"
                     /translation="MNKELSRRQFIGTTATALAGFTILPGFGIGGVRRAPSDKLNIAG
                     IGVGGVGGTNIANCSGENIVALCDVDWSYAKATFEKYPNARIFKDYRKMFDEMKGGID
                     AVVIATPDHTHAIIAAEAMRRGMHVYLQKPLTHSVSESRLLTQLAREYRVATQMGNQG
                     NSDEGIRQLCEWLWDGAIGEIREAHAWTNRPIWPQGLERPLETEAIPPTLDWDLFLGP
                     APLRPYHSAYHPWNWRAWWDFGTGALGDMGCHIFDPVFKALRLRNPQYLYGSSTQVNT
                     ESAPKASVVHYEFPDRGEYKNLKLVPVHVTWYDGGLLPPRPAELKDGEPFGDWSGGCL
                     FIGTKGKLLCDSYGRNPRLLPEQLDKDYVRPVPYLRRIEKAMDGGHELDWIRACKESP
                     DSRTECSSNFDYAGPMNEVVVMGNLAVRLQDLKRKLQWDAEKMMIPNISDDDEIRVVT
                     TDKFTVINGHPHFDTQYATLKAKPAAEEYIRHTYREGWKL"
     gene            4454..5131
                     /locus_tag="PKZ74_02285"
     CDS             4454..5131
                     /locus_tag="PKZ74_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM91608.1"
                     /translation="MSQGKENMLTKKEKKEGWALLFDGKSANGWRGYNKTAFPEKGWA
                     VEEGTLHVIGSSRGEAGGGGDLLFDKKFRNFELSLEWKVSEGGNSGIFYLAQEIPGEP
                     VWKSAPEMQILDNEKHPDAKLGVDGNRAAGSLYDLIPGDFKAVKPAGQWNQVRIMVYK
                     GTVVHYVNGKAVLEYHLWTEDWKNMVLNSKFKDYEWFLNTAEEGYIVLQDHGDDVWFR
                     NIKIREM"
     gene            5173..6663
                     /gene="amrB"
                     /locus_tag="PKZ74_02290"
     CDS             5173..6663
                     /gene="amrB"
                     /locus_tag="PKZ74_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPM91609.1"
                     /translation="MILLPLFFGSGSHAQDGGSKSVTVDRKPAVAGQFYPSDPVRLRT
                     GLSALFAAAQPCETKNTVAIISPHAGYVFSGEVAASAFNQIDPDKNYENIFILASSHR
                     MSFDGASVYTRGDYITPLGAVKVNRELASSLVKENPVFTDVPDAHLYEHSLEVQLPFL
                     QYRMKTEFRIIPIVLGTQSAASANKIAQALKPYLNDRNLFIISTDFSHYPEYDDACLV
                     DKATAGAIIKNSPEHLLKLLEENDRKGIDDLATSLCGWTSVVTLLYMTEERSDLIYRE
                     IKYMNSGDSPLYGNKDRVVGYFAIAVEQSSLSKQNDSFSLNEEERKLLLQIARQTMEQ
                     YVSKGKVAPVDEKDLPARLREQAGAFVTLKKDGQLRGCIGRFEATSPLYDVVQQMAVA
                     SSTQDSRFEPVETDEFDDIDIEISVLSPMRKISSVDEIVLGKHGIYMKKGFYSGTFLP
                     QVATETGWTLEEFLGHCARDKARIGWDGWKTAELFVYEAYVFGEKE"
     gene            complement(6665..9535)
                     /locus_tag="PKZ74_02295"
     CDS             complement(6665..9535)
                     /locus_tag="PKZ74_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPM91610.1"
                     /translation="MTPFLQRLAREIVRMPVDDPGKVCVVLPNRRAGLYLKKYLAKEL
                     QKAAWAPATYSIEDFITTISGFGMADPAGLLFEFYKVYTDNVREKAQDFEHFTDWAEV
                     LLQDFDEIDRYMADPEKVFHYLDAARALSVWNLNAAPLTQQEQDYVEFYRSLNDLYRE
                     LRSRLTAQKQVYQGLAYRLTAENIEEIRKTLPWNKIIFAGLNALSVAEVQIIDSLVSG
                     SHAELFWDADAYYTDNPLMEAGVFLRQHFQKWPREKQSWIGTDFRDGAKKIKVTGIPG
                     IAGQARLAGDIIMKMKMDAEAPDHTAIVLADEKLLLPVLYTLPEDIGPVNVTMGYPFR
                     LTQLYHLISLFFRMQETAIQLSERRRSTDKAFYVKDILKLLAHPYLQMFAPESGSDAP
                     DFRQIADILKNRNRIFLRPGELLKFSASSGDEFRNLNELLFQFWESPSSGLRCLMALT
                     GLIRDKVIAMDSQPYIDRDLDIEYLYHFSGILNRSIAMAEQYPFIKSVSSLKKILFRL
                     LDNSRLPFTGEPLRGLQVMGVLETRAIDFENIVVLSVNEGILPSGRVPNSFIPFDIKT
                     EFGLPTFRQKDAVFAYHFYRMLQRAENIHLIYDTEGDQMKGGERSRFIMQLGFELKKY
                     NPGISFDESLEKPSSELSGNVKEIIIPKSETILERLSTKTLSGFSPTAFSLYITCPLK
                     FYFQEVLGLAETESVEETIESKTMGTVIHQVLFQIFEPFTGKAVDAQSLTAGLGAIET
                     LTHKAFRKHYPEGDTELGKNHLILRVVLLFLRNFIKSEIEQLAATDEKKSRLEILSLE
                     KKLEYILTCHPEGKTYNVKIKGHVDRIDRINGMTRIIDYKTGSVQARELSLKDWDQLS
                     TDPKTGKALQLLIYGWLYLKNKTSDSTDLRVGNISLRRISAGFIPVKVPNEQGFQDDT
                     SDQIEKQLIKLLEEILDPGVPFRQTADAANCKYCPFTSICTR"
     gene            complement(9532..10278)
                     /locus_tag="PKZ74_02300"
     CDS             complement(9532..10278)
                     /locus_tag="PKZ74_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM91611.1"
                     /translation="MKALILAAGLGTRLYPLTADTPKALVQVNGMTMLERAIRKLASA
                     GFNEIVVNVHHHAEKIKDFLRDLDLPGITTAISDESDLLLDTGGAILKARPFLDDRDP
                     FLVHNVDVISNIDLQALLIDHLECGNMATLSVIDRPTKRYFLFDSEMGLRGWTDTSTG
                     EIRWTGRPAVNVSRLAFSGIHIISPAFFKVLRQSGRFSIIQTYLDMAVQHPIRGHTQE
                     GAVWFDLGKPEQLTMASEYIKNHPELNIYP"
     gene            complement(10275..10418)
                     /locus_tag="PKZ74_02305"
     CDS             complement(10275..10418)
                     /locus_tag="PKZ74_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91612.1"
                     /translation="MSKKICKEDDRKVIAKKVKKAEFECKTCGAGANKEKYLCKPKRI
                     SGR"
     gene            complement(10423..11853)
                     /locus_tag="PKZ74_02310"
     CDS             complement(10423..11853)
                     /locus_tag="PKZ74_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPM91613.1"
                     /translation="MQTITDIIYKLYRQWAGKDPESIKDLPPAGSKRKYFRVISGDTT
                     AIGAWNPVIEENEAFFHFSEHFRRNGLHVPEIYAIDPGREIYLLEDLGDISLFSVVDK
                     STNQEITAGIKNLYRKSLSELVRFQVTGHQGLDYSLCYPRQAFDKRTILFDLNYFKYY
                     FLRLHLDFHEDRLEDDFITFTDYLLKTDGNFFMYRDFQARNILIKNNQPYFIDYQGGR
                     KGPLLYDVASLLFQVKADLTFSLRKELLEFYLEELSEMMVIDKTSLRKIYEGFVLIRL
                     LQVLGAYGFRGLIEKKPHFIASIPYALKNLHWWIDQDSFELAIPELRRALAGLAALTQ
                     YRLPEISGSGKQSLTVEIKSFSYKNGYPVDLSKNGGGFVFDCRALPNPGREERYREFT
                     GKDREIIDYLKDEPAVIAFLDEAEKLVGQSVENYIERGFQNLSVSFGCTGGQHRSVYC
                     AETLAGRLRRRFPAVGFPVTHTNLSD"
     gene            complement(11936..12430)
                     /locus_tag="PKZ74_02315"
     CDS             complement(11936..12430)
                     /locus_tag="PKZ74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM91614.1"
                     /translation="MKDLPHYFAIVLILFMGLTGCVNVENKKPGVNDEIATITQTIHN
                     SIGWAKDKDLDMLYAVIANDSAFLEVHPNGRVVKGIGQFRTAEEFWMSPDFKAIRYEI
                     RDLTVSMSESGTVAWWFCVLDDITEYKGRPACWENTRWTGVAEKRDGNWVIVQQHFSF
                     AEGK"
     gene            12742..13242
                     /locus_tag="PKZ74_02320"
     CDS             12742..13242
                     /locus_tag="PKZ74_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPM91615.1"
                     /translation="MAKSAVDILKEAILLERRGKAFYTTAARQTQSEAARKIFEMMAA
                     EEDEHIEFLSKQFAYYEQNKSFMKAQPHEDTDEATVMAILSEEIKSQVNAAGFEAAAI
                     SAAMDFETRAVQIYSERAQSATDENERETYQMLADWEKGHHHWLHKINEDLKEQIWYD
                     NNFWPF"
     gene            13252..14436
                     /locus_tag="PKZ74_02325"
     CDS             13252..14436
                     /locus_tag="PKZ74_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPM91616.1"
                     /translation="MSSAKLDFESKLIHEGGFEDELGSAVTPIYQASTFKFRNADHGA
                     QCFSGESDGYIYTRLGNPTIKELEKTLAALENGFGGIATSSGMGAVNTVYMAYLGQGS
                     HVICHEAVYGPSRVVLETIYTRFGVEYTMVDTTDIEKVKTAFRPNTKLLFTETPANPT
                     MGISDLPALAELAHSHNVPLVVDNTFCSPYLQNPLDLGADIVLHSMTKFINGHADIVA
                     GMIVAKKEEDYKKLRGIMTNLGCNMDPHQAFMTRRGLKTLAIRIDRAQENAMVIAGYL
                     EKHPKVAWVKYPGLKSHPQYELGKRQMKGPGAMISFELKGGLTAGKVLMDNVKVMLLA
                     VSLGGIETLIQHPASMTHSKVSAENRHHAGITDGLVRLSVGIEKVDDLLADLDQALGK
                     II"
     gene            14636..16960
                     /locus_tag="PKZ74_02330"
     CDS             14636..16960
                     /locus_tag="PKZ74_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPM91617.1"
                     /translation="MTEKDLTADNIQQELPENTEITSEPSATPEAETTEIPAEEILTE
                     QAEIQEIITETIPTEEIPVQDIVPDDSPLAEALTDDVPVEISPAEVIPEVIAPVEEIP
                     VEEVPTENASDPEPAVEDVPVEEVAAEDVPEEKPVAEDAEPEPVAAEEQTATELHPEV
                     TADAALLQEIAEMDHSGEQETEEEEEDILEEHEEIVERYDTYDREKLVEILEETVKEE
                     NIVRIKTRVAMIKVAFLRLSKEFRQQLLDNHLTAGGTRENFTPEPDPLVEKFNAVFET
                     YKKNKIKFNEHQEVVKLRNLDAKKAILEELKELINSEETLKKTYDQFKELQTKWKEIG
                     LVPSTEINNLWQNYHFLVEKFFDKVKISKELRDLDLRKNLEHKIELCEKAEELLIEPS
                     IIKSFQQLQKYHEEWKEIGPVPQDKRDEIWERFKTATDKINERRREHYNILAKDQDEN
                     LAAKNALVEKAEQLASIEVDELKGWQEKTREVNELFRQWKTIGPAPKKENDEVWEKFK
                     AYLNAFFANKKEYFSRIKQEQLNNFNLKLDIVKQAEALKDSTDWRKTTQDLINLQKEW
                     KNIGPVPRKHSDKIWKRFRAACDEFFNNKSKHFSTVVESEKDNLDKKLALIKQVEEWE
                     AGKDKSANLEALKGFQREFMEIGHVPIKEKNKVHDAFRQAINKHFDNLKLSSYEGRPQ
                     SEYREYRSRQDSYDGDRYQRRGGSTGGGSSEWSALSTKVSKLREEIMLWENNIGFLAH
                     SKKADLLRAEFEKKIQDAKAELASLEAKLKSMQE"
     gene            16967..17956
                     /locus_tag="PKZ74_02335"
     CDS             16967..17956
                     /locus_tag="PKZ74_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPM91618.1"
                     /translation="MSGNSIGISFKLTTFGESHGPATGGVIDGCPAGLPIDPEFIQQE
                     LDRRKPFYEGATGRKEADAVEFLSGIGNGISLGTPIAFIIRNEDVITSDYQEIKDVFR
                     PSHADYSYFMKYGRMATGGGRASGRETAARVAGGAVAKLLLGKAGISVKGKVQSIGDG
                     TAEGISKAIEQGDTLGGTVTCRIAGVPAGLGEPVFDKLSAALAKAAMSIPSAKAFEYG
                     LGFMAPTMPGSKYNDQMTVKDGKAVFLTHHDGGIQGGISNGDDIYFRVAFKPVPSVKI
                     PQDTINKDFEPVRIEIGGRHDVCHVPRLVVVVESMAALVLADHWLRAMNSRLA"
     gene            complement(17953..>18898)
                     /locus_tag="PKZ74_02340"
     CDS             complement(17953..>18898)
                     /locus_tag="PKZ74_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HPM91619.1"
                     /translation="HGLVENSFFDPQTGKHYSINNRAAVEDASFYGGEPIWVTAEKQG
                     ITSASFFWVGSEAKGRHPSYWKKYEHEFPFSQRIDTVIYWLSLPEEKRPHLVLWYFDE
                     PDGTGHSEGPLSEATCRMVTTLDSLVGVFMARLETLPHAGDINVIFTSDHGMGPTHKE
                     RCIMLKNYLDYGWFEEMEGYTPNLTLKVREEFRDTAWKALSAIPHITVWKHGEVPDSL
                     HYGTNPRTLDFILVADSAWQFSLTDKILRTGGAHGYNPYNTDMHGIFFARGPAFRQNY
                     RHPTFEHIHLYPLICEILGLEPAPVDGRVENIQGMLKD"
BASE COUNT         4881 a         4665 c         4855 g         4497 t
ORIGIN      
        1 tgctgaaagc cggcgcaatg ccgggttcgg aagactgggg taaggaagat cccgattact
       61 ggggattgtt gaatgcagag atgcacgggc agcagttcta cgggaatatt gaaacggaac
      121 ccggaaatta tatggctttt tacgataatg tttttgatgt gatcaccaaa ggtgcggagc
      181 aggccgttac accccaggaa gcacggaatg tcatccgcat ccttgagctg gcatttgaga
      241 gccaacgcgc gcaatcactg gtcaaaatag cctgagcgtc attcccgcgc cagcgggaat
      301 cctttttctt gacaccaaat ctacctccct cccggagggc agttcgattt taagaaatcg
      361 gtgtaaagcg ttgacaggtg caacaaagtg ccttcccctt cgtagattcc gtgtgtgcgg
      421 ttgggatagg gcatgaactg gaattgcttg ttgtgtttga ccagctcgtt cagcagcatt
      481 tcggcgttct ggtaatggac attatcgtcg cctgttccat ggatgatcag caggttgcct
      541 tccaggttgc gggcatactt taacggcgat ccgttctcaa aatcttccgg gttttcctgc
      601 ggcaggccca tgtagcgttc ctggtaaatg ttgtcgtacg tcagcaggct ggaaacggat
      661 gcaatcgcta tccccgtctt atagatctga ggatactgga acatcaggtt cagggtagcc
      721 gagccgccgc cgctccatcc ccacacggcc actctttccg gatcgatgaa gtcccatttc
      781 aggatctctt ccgccgccat tgcctggtcg cggatgttga gctgcccgat ctgccggtaa
      841 atcgatttcc gccattctct gcctttaggc gccggcgtac cccggttatc cagtgaaata
      901 tagatgtagc cgtcttcagc catgtcgcct ccgtacaacc cgttctcact gatgccgtaa
      961 gtatcggtta ccgttgtcga tgcaggctcc gtgtaaacga aaaaaacaac gggatatctc
     1021 tttttcggat caaaatccgt gggtttggcc atccaaccat ccagttcaac cttgtctttc
     1081 gtccggatcg taaagaattc aaccttgggt gtttctttca gcgagtcaag gttcagaaca
     1141 atactttcat tttcgaacaa cggtttatgg tcgggtaaag cgatcagttc tttagacggt
     1201 tgggtgtaat aatttgaaaa tgaatgaaaa gcgtattttc catcacccga gaacgaataa
     1261 tcatgcgtcc ctttaagtat ttccgggctc agcagttcgg gttttccggg atcatcagca
     1321 tctacgcggt agaggtattt ctgtgtggca ttatccggcg aagccataaa ataaatgtca
     1381 tgcgttgccg gatcaatgaa tttaaggtcg atgacatcgt attttcccct ggttatcagc
     1441 ttttccggcg atccatcgag gtaaacttca tagagatgcc tccagccgtc cttttccgac
     1501 gaccagataa tgctcctgtc tttatccacg aaattgaatg agttggtata atcgacagta
     1561 taaggatttc cgggttgaaa aagttcgacc caggcttcat cggtctcttc attcaataat
     1621 tgtgtgttgc cggttttggc atccgccatg aataacttgc tgtggttctg tttccggttc
     1681 agttgctgta gcaacaattt ttccgttccc gggatgaatt ccagcctgac gatataatgc
     1741 tggaccggat cgcccggaat atccatccag gtcgttaaac cattttctaa actgacaacc
     1801 ccgattttgc aggatgaggg atcctctccg acctttggat attcgatcgg aataacctgt
     1861 gaataaatgg aatcggtgtt gttgatcatg taaaatttcc tgatattgcc ggcatcgagt
     1921 tgccagtagg cgatggattt gctgtcggga ctccatctga aaccgtcgcg gcagaaaaat
     1981 tcctcttcat acacccagtc gaacgtgccg ttgatcaggg taaccgttcc gtcggtcgtg
     2041 aggggcttga tgagatgggt tgccaggtct tccgcataga ggttgtttcc gctcacgtag
     2101 gctaccgatt ttccgtcggg ggaaaacttg gcaaacatga gtgaggaggc cggcaacgat
     2161 ttgccaagct gggtaaaagt accggcagtc cggtcgagca cccagtaatc ccctttggtg
     2221 ttaagccgcc agaccctttt cgtgttagtg aagataagca gtttctgttc atcatcggaa
     2281 aaggagaaat aattgaactt caacgcactg ctgctgcctt caggggtaag ttgtttttta
     2341 gtgatgatga cttcgggtgt attttccggc agtgtgtacc ggataatttc atttttttca
     2401 accttaaagt acgcacttcc ttccctggcc cacttgaccc ctctttgctg agtataagcc
     2461 gggatctgga tcaggatgat gattacgaaa aggacaatgg cctttagcca gatctttttc
     2521 aataagccga ttttattagt tttcataatg atttttttaa tgagataaaa ttaggggaat
     2581 attcagatgt atatcctgaa aaatgacgac cagcaacatt ttaccttaaa aatatttacc
     2641 tttacctgac cgaaaatctc acatacaata caaccttatg accactttat gaccactcta
     2701 taaaacaaac aaactaaacc aaagaaaaag atcaataacc aacaatttaa tacctatgaa
     2761 taaagaacta tcacgcagac aattcatcgg gacgacggcc actgccctgg caggattcac
     2821 cattttgccg ggtttcggga taggcggagt ccgccgggcg ccgtccgaca aactcaatat
     2881 cgccggtatc ggtgtcgggg gagtgggtgg caccaacatt gccaactgca gcggcgaaaa
     2941 tatcgtggca ttatgcgacg tcgactggtc ttacgccaaa gcgacctttg agaaatatcc
     3001 gaacgccagg atttttaagg attaccgcaa gatgttcgat gaaatgaaag gggggatcga
     3061 cgccgttgtc atagccacgc ccgaccatac gcatgccatc attgcagcag aagccatgcg
     3121 ccgcggaatg cacgtctact tgcagaaacc gctgacacac agcgtatctg aatcacgtct
     3181 tctgacacaa ctggcccggg aataccgggt ggccacccag atgggcaacc aggggaactc
     3241 tgatgaaggc atccgccagc tttgcgaatg gctgtgggat ggcgccattg gcgaaatccg
     3301 tgaggcccat gcctggacca accgcccgat ctggccgcag ggcctcgaac gtcctctgga
     3361 aacagaagcc attccgccca cactcgactg ggatcttttc ctcggtccgg ccccgctgcg
     3421 tccttaccac tcagcgtacc atccctggaa ctggcgtgcc tggtgggact tcggcaccgg
     3481 cgcactgggc gatatgggct gccatatctt cgacccggtc ttcaaagcgc tgcggctccg
     3541 taatccgcaa tacctctacg gaagctccac gcaggtcaat accgaaagcg cacccaaagc
     3601 ctcggtcgta cattacgaat tccccgaccg gggagaatat aaaaacctga aactggtgcc
     3661 ggtccatgtt acctggtacg acgggggcct cctcccgcca aggccggctg agctcaaaga
     3721 cggcgagcct ttcggcgact ggagcggcgg ttgcctcttt atcgggacca aaggaaaact
     3781 gctctgcgat tcttacggcc gcaatccgag gttactgccg gaacagctcg ataaagacta
     3841 tgtccgcccc gtgccatacc tgcgaaggat cgaaaaagcg atggatggcg ggcatgaact
     3901 cgactggatc agggcctgca aagagagccc agacagccgg accgaatgca gctccaactt
     3961 tgattacgca ggaccgatga acgaagtggt ggtgatgggt aacctggctg tccgcctgca
     4021 ggacctgaaa cgtaaactgc agtgggatgc cgaaaaaatg atgatcccga atatttccga
     4081 tgatgatgaa atcagggtgg taaccaccga taaattcact gtcattaacg ggcatccgca
     4141 tttcgacacc cagtatgcca cgctcaaagc caaaccggca gcagaggaat atatccggca
     4201 tacgtacagg gaagggtgga agctgtgaga tttggcgatt tgacgatttg gcgatttgat
     4261 gatttgatga tttggtgatt tggtgatttg gcgatttgat gatttggtga tttggtgatt
     4321 tggtgatttt tagctttaaa tagtctgtaa agtctgtgaa tgaatctgtg taaatctgtg
     4381 gtgaataaaa taataataga atatgaaaac aacaatgatg attataatct tatcgatgat
     4441 tacctctggc gcgatgagcc agggaaagga aaatatgctg actaaaaaag aaaaaaaaga
     4501 gggctgggcg cttctgttcg acgggaaatc ggctaacgga tggcgtggat acaacaaaac
     4561 ggctttccct gaaaaaggat gggctgtcga agaaggaacg ctgcatgtga tcggctccag
     4621 caggggagaa gcgggcggag gcggcgacct gctgtttgat aaaaaattcc gcaacttcga
     4681 actgtcgctc gaatggaaag tgtcggaagg tggcaacagc ggcatcttct acctggccca
     4741 ggagattccc ggagaaccgg tctggaaaag cgccccggag atgcagatcc tcgacaatga
     4801 aaagcacccg gatgcaaagc tcggagtcga tggaaaccgg gctgccggct ctctgtacga
     4861 cctgatcccc ggtgatttta aagccgtgaa gcctgcagga cagtggaacc aggtcaggat
     4921 catggtctat aaagggaccg ttgtccatta tgtcaacggc aaagcggttc tggagtatca
     4981 cctctggacc gaggactgga aaaatatggt attgaacagc aaattcaagg attatgaatg
     5041 gttcctgaat acggccgaag aaggttatat tgtgttacag gatcatggag acgacgtctg
     5101 gttcaggaat attaagatca gggagatgta atatagatat ttccaatgaa gtgtagacta
     5161 agattgaccg gtatgatcct attgccttta ttctttggtt ccggcagcca tgcccaggat
     5221 gggggcagca agtccgtaac ggttgaccgg aaaccggctg tggccgggca gttctatcct
     5281 tccgaccctg tccggttgcg caccgggctc tccgctttgt ttgccgctgc gcagccgtgc
     5341 gaaaccaaaa atacggtggc tatcatttcg cctcatgccg gatatgtttt ttcgggcgag
     5401 gtggctgctt ccgcctttaa ccagatcgac ccggataaaa attacgagaa catcttcatc
     5461 ctggcatcca gccaccggat gagcttcgac ggcgcttcgg tttatacccg aggagactat
     5521 atcacacctt tgggtgccgt aaaggtcaac cgcgaactgg cttcatcact ggtgaaagaa
     5581 aatccggttt ttacggatgt tcccgatgcc cacctctatg agcacagcct ggaggtccag
     5641 ctcccttttc tccagtaccg gatgaagacc gagttccgga tcatccccat cgtgctgggg
     5701 acacaatcgg ctgcatcggc aaacaaaata gcgcaggcat tgaaacctta cctgaatgac
     5761 cggaacctct tcatcatcag taccgatttt tcacactatc cggaatatga tgacgcttgc
     5821 ctggtcgata aagccactgc cggtgccatt attaaaaatt caccggaaca cctgcttaaa
     5881 ttacttgaag aaaacgaccg gaaagggatt gatgacctgg ctacaagtct ctgcggctgg
     5941 acttccgtgg tgacattgct ttacatgaca gaagaaagga gtgacctgat ttacagagaa
     6001 ataaagtata tgaactccgg cgattcgccg ctgtatggca ataaagacag ggtagtgggt
     6061 tattttgcta ttgccgtaga acaaagttct ctgtcaaagc agaacgactc cttcagcctg
     6121 aacgaggaag aaaggaagtt gctgcttcag attgcacggc aaacgatgga gcaatatgtt
     6181 tcgaaaggca aagtcgcacc tgtcgatgaa aaagacttgc cggccaggct ccgggagcaa
     6241 gctggcgctt ttgtcacact aaagaaagac ggtcagctcc gcggttgcat tggccggttt
     6301 gaagcgacca gcccgttgta cgacgtggtg cagcaaatgg ccgttgcatc ttctacacag
     6361 gattcgcgtt tcgaacccgt tgaaaccgat gaattcgatg atatcgatat tgagatatcc
     6421 gtcctttcgc ccatgcggaa gatcagctca gttgatgaaa tagtactggg aaaacatggc
     6481 atctatatga aaaaggggtt ctattccggc accttcctgc cccaggtggc tacggagacg
     6541 ggatggaccc tcgaggaatt ccttggccat tgcgcccgcg ataaagcccg gatcgggtgg
     6601 gacggttgga aaacagccga attgtttgtt tatgaagcat acgtgttcgg ggaaaaggaa
     6661 tagcctaccg ggtgcagatc gatgtaaaag gacaatattt gcaattggca gcatcggccg
     6721 tctgacggaa cggtaccccc ggatcaagta tctcctccag caacttaatt agttgttttt
     6781 caatctgatc tgatgtatca tcctggaatc cctgctcatt gggaacctta accggtataa
     6841 acccggcgct gatccggcgc agcgaaatat ttccaaccct cagatcagtg gaatccgatg
     6901 ttttgttttt caggtaaagc catccataaa tcagcaactg gagcgctttt cccgttttcg
     6961 gatcggtcga gagctgatcc cagtctttga gactgagctc ccgggcctgt accgagccgg
     7021 ttttgtaatc tatgatccgt gtcatcccgt ttattctgtc gatcctgtcg acatggcctt
     7081 tgattttcac attgtatgtc ttaccttccg gatggcacgt cagaatgtat tccagttttt
     7141 tttcaagtga aagtatctcc agccgcgact ttttttcatc cgtggcggct aattgttcaa
     7201 tttcgctttt aatgaaattc cggaggaaca acaaaaccac acgcaggatc aggtggttct
     7261 tcccaagctc cgtatcccct tccggataat gcttccggaa cgccttatgg gtgagggttt
     7321 caatagcgcc caggccggca gtcaggctct gagcatcaac tgctttgcct gtaaatggtt
     7381 cgaatatctg aaataatacc tggtggatga ccgttcccat ggttttcgat tcgatggtct
     7441 cttcaaccga ttccgtttcc gcaaggccca gcacttcctg gaaatagaat ttaagcgggc
     7501 aggtgatgta taggctgaac gcagttggcg agaacccgga aagcgttttg gtgcttaacc
     7561 gctccaggat ggtttccgat tttgggatga ttatttcttt cacatttccg gataactccg
     7621 acgatggttt ttccagggat tcgtcgaatg agatcccggg gttgtatttt ttcagttcaa
     7681 aaccaagctg catgatgaaa cggctgcgtt cgcccccttt catctggtcg ccttcggtgt
     7741 cataaataag atggatgttt tcggcccgtt gcagcatccg gtaaaaatga taggcgaata
     7801 ccgcatcttt ttgcctgaat gtcggaagcc cgaattcagt tttgatatcg aacgggataa
     7861 atgaattggg gacccggccc gacggcaata ttccttcatt aacagacagc accacgatgt
     7921 tttcaaagtc aatggccctg gtttccagaa cccccatcac ctgcagccct ctcagcggtt
     7981 cgccggtaaa gggaagccgg ctgttgtcga gcaagcggaa aaggatcttt ttcaggctgc
     8041 tgaccgattt gataaaagga tactgttcgg ccatggcgat actccggttc aggattccgg
     8101 aaaaatgata gaggtattca atatcgaggt cgcggtcaat gtaaggctgg gagtccatag
     8161 cgattacctt gtcgcggatc aggccggtca gtgccatcaa gcagcggagc ccagaggatg
     8221 gggattccca gaattgaaac aaaagttcgt tcagattccg gaattcgtcg ccggagcttg
     8281 ctgaaaattt caacagctca cccggacgga ggaaaatacg gttcctgttt ttgagtatat
     8341 cagctatttg ccggaaatcc ggggcatcgc tgccggattc gggagcgaac atttgcaggt
     8401 acggatgggc cagaagcttt agtatatctt tgacgtagaa tgctttatcg gttgatctcc
     8461 tccgttccga caactgtatg gccgtctcct gcatccggaa gaacaggctg atcagatgat
     8521 agagctgggt aagacgaaaa gggtagccca tggtcacgtt caccggtccg atatcttccg
     8581 gaagggtata aagaacgggc agtagcagtt tttcatctgc cagtacgata gctgtatggt
     8641 ctggagcttc cgcatccatt ttcatcttca tgataatatc cccggccagc cgtgcctgtc
     8701 ctgctatacc cggaatgccc gttaccttga tttttttggc cccgtcacgg aaatccgtac
     8761 cgatccagga ttgtttttct cgcggccatt tctgaaaatg ttggcggagg aaaacacctg
     8821 cttccattaa cgggttatcc gtgtaatagg catcggcgtc ccagaaaagt tcggcatgag
     8881 atccacttac caggctgtcg atgatttgca cctcagccac cgacagggca ttcagcccgg
     8941 cgaaaatgat tttattccag ggaagggttt tccggatctc ttcaatattt tcggctgtta
     9001 accggtatgc cagaccctgg taaacctgtt tttgggctgt cagacgagag cgcagttcac
     9061 ggtacaggtc gttcagcgac cggtagaatt cgacatagtc ctgttcctgc tgtgtcagcg
     9121 gagcggcatt gaggttccag accgacaaag cccgggctgc atccaggtaa tggaacactt
     9181 tctccgggtc ggccatataa cggtcgattt cgtcaaaatc ctgcaggagt acctccgccc
     9241 aatcggtaaa gtgttcaaaa tcctgtgctt tctccctcac gttatcggtg tacaccttgt
     9301 agaattcgaa tagcaaaccg gccgggtcgg ccattccgaa acccgatatc gtggtgataa
     9361 aatcttcaat ggaataagta gccggggccc aggcggcttt ctgcaactct ttggccaggt
     9421 attttttcag gtagagtccg gcacggcggt tgggcaaaac aacgcacacc ttaccgggat
     9481 catctaccgg catccggacg atctcccggg ccaaccgttg aagaaaaggg gtcatggata
     9541 tatattcagt tcgggatgat ttttaatgta ctctgaagcc atggtaagtt gttccggctt
     9601 gcccaggtcg aaccagaccg caccttcctg tgtatgtccg cgaatcgggt gctgcacagc
     9661 catatcgaga taagtttgga tgatgctgaa tctcccggat tggcgcaaca ctttgaaaaa
     9721 agccgggctg ataatatgga tgccgctaaa cgccagccgt gaaacgttga ctgccggccg
     9781 gccagtccac cggatctcgc cggtcgaagt atcggtccat ccccgcaatc ccatttcgct
     9841 gtcgaacagg aagtaacgct ttgtcggccg gtcgatcacc gaaagggtag ccatattgcc
     9901 gcattcgaga tggtcaatta acaaggcctg aagatcaata ttgctgatca catcaacatt
     9961 atgcaccagg aaaggatccc ggtcgtcgag aaaagggcgc gctttcagaa tggcgccgcc
    10021 ggtatcaagc agcaggtccg attcatcgga gatggctgtt gtaatgcccg gcagatcaag
    10081 gtcccggagg aaatctttga tcttttcggc atgatggtgc acattgacga caatctcatt
    10141 gaaacctgca gaggccagct tccggatggc ccgttccagc attgtcatcc cgttcacctg
    10201 caccagcgct ttgggggtat cggccgttag cggatagagc cgggtgccca gcccggcggc
    10261 taaaatgagc gctttcatcg gccggaaatt cttttgggtt tgcagaggta tttctcttta
    10321 tttgccccgg ccccgcaggt cttgcattcg aattcggctt tcttcacctt tttcgcgatc
    10381 actttccggt cgtcttcctt gcagatcttt ttcgacataa aatcaatcac ttaaattagt
    10441 atgtgtgaca gggaaaccga ccgcagggaa tcgccggcgc aatcttccgg ccaaggtctc
    10501 cgcacagtag accgaccggt gctgcccgcc ggtgcagcca aaacttaccg aaaggttctg
    10561 aaatccccgt tcgatatagt tttcgaccga ttgtccgacc agtttctccg cttcatccag
    10621 gaaagcgatt acggccggtt catccttcag gtaatcaatg atctcccgat ctttccccgt
    10681 aaactccctg tatctttcct ccctgcccgg gttgggcaag gcgcggcaat cgaacacaaa
    10741 accaccgccg ttctttgaaa gatcgacagg gtagccgttt ttgtacgaga aacttttgat
    10801 ttccaccgtc aaagactgtt taccggaacc cgaaatttcg ggaagccggt attgtgttaa
    10861 tgctgccaga ccggccagtg cccggcgtaa ttccggaata gctaattcaa atgaatcctg
    10921 gtcaatccac caatgcaaat ttttaagcgc ataaggaata ctggctatga aatggggttt
    10981 tttttcgatc agtccgcgga aaccgtaagc gcccagcacc tgtaagagcc gtatcagcac
    11041 aaatccctca tatatttttc tgagactggt tttatcaatc accatcattt cagataattc
    11101 ttccagataa aattcaagca attcttttct cagtgaaaaa gtcagatcgg ctttcacctg
    11161 gaagaggagc gaggccacgt cgtaaagcaa tgggcctttt cttcctccct gataatcaat
    11221 aaaatagggt tggttattct taatcaggat attgcgtgcc tggaaatcgc ggtacataaa
    11281 gaagttgcca tcggttttca ataaataatc ggtgaaggta ataaagtcgt cttccagccg
    11341 gtcttcgtga aaatcgagat ggagtctcag aaaataatac ttgaaatagt tgaggtcgaa
    11401 taatatagtg cgtttatcga atgcctggcg gggatagcac agcgaataat cgagcccttg
    11461 atgtccggtc acctggaacc ggaccagttc gctgagcgat ttgcggtaaa gattcttaat
    11521 ccctgctgta atctcttgat ttgtcgattt atcgacaacc gaaaacaggg aaatatcgcc
    11581 caggtcttcc agcaggtaga tttcccgccc gggatcgatg gcatagattt cagggacatg
    11641 cagtccgttg cgacggaaat gctctgaaaa gtggaagaag gcttcgtttt cctcaatcac
    11701 cgggttccag gcgccgatgg ctgtcgtatc gcctgaaatg acccggaaat attttcgttt
    11761 cgatccggcc ggcggcagat ctttgatgga ttccggatct tttccggccc actggcggta
    11821 gagcttataa attatatcgg taatggtttg caatactgaa aattttgccg gaataaaggt
    11881 acaaaactat acaatctcca ccggcccctt tacgccttca aaaccatttc ccgatttatt
    11941 tcccttctgc aaacgaaaaa tgttgctgta cgatcaccca gttgccatct cttttctctg
    12001 ccacgcccgt ccagcgggtg ttttcccagc atgccggccg gcctttgtac tcggtgatgt
    12061 cgtcaagcac acaaaaccac caggctaccg tgccagattc tgacatggaa acagtcaaat
    12121 cacggatctc ataacggatg gctttgaaat cggggctcat ccagaattcc tccgccgtcc
    12181 tgaattgacc gattcctttc actactctgc cgttcggatg gacttcaagg aaggccgaat
    12241 cattggcgat gacagcgtat aacatatcaa ggtccttgtc tttggcccag ccgatgctgt
    12301 tatggatagt ttgggtgatt gtggcaattt catcgttaac gcctggtttt ttgttttcaa
    12361 cgttgacgca gccggtcaga cccatgaaga gaattaacac tatggcaaaa tagtgaggga
    12421 gatctttcat ttaatctaag tttttgtaaa gataaggacc tcatgggttt atagacataa
    12481 ttaaactgca aaccggtgtt tcaggtttac aaattgcgac attttatttt gctctcagtt
    12541 ccctcccccc cggctggcga gggaattatc ggttccgggt ttcaacgaga ctccatctca
    12601 actggccaga tggagtctcg aaacaacgag atggagtctc acatcaacga gatggagtct
    12661 catgccattt tactcctttg aaatcgacga atcttcgcgg atttgtttat atttgcctgg
    12721 caattataaa accaatgaaa tatggcaaaa tcagccgttg atatcctgaa agaagcaatc
    12781 ctgctggaac gcaggggaaa agcattttat accaccgcag cccggcagac gcagagcgaa
    12841 gcggcccgga aaatattcga aatgatggcc gccgaggaag acgaacatat cgagttcctt
    12901 tcaaagcaat ttgcttatta tgagcaaaat aagtccttca tgaaggcaca gccgcatgaa
    12961 gataccgacg aagccactgt catggcaatc ctctcggaag agatcaaaag ccaggtgaat
    13021 gcagccggtt tcgaggccgc agcgatctcc gcggcaatgg acttcgagac aagggccgtt
    13081 cagatatatt ccgaacgcgc tcaatcggcc accgacgaga acgaacgcga aacctaccag
    13141 atgctggccg actgggaaaa agggcaccac cactggctgc ataaaatcaa tgaagacctg
    13201 aaggaacaga tctggtacga caataatttc tggccttttt aaccaaacga aatgagctcc
    13261 gctaaacttg attttgaatc gaaactgatt cacgaaggag gatttgaaga cgaactgggc
    13321 agcgctgtaa cgcccatcta ccaggcctcc acattcaaat tccgcaatgc cgaccacggc
    13381 gcccaatgct tttcgggcga aagcgacggc tatatctaca cccgcctggg caacccgacg
    13441 atcaaagaac ttgaaaaaac actggccgcg ctggagaacg gtttcggtgg cattgccaca
    13501 tcttccggca tgggtgccgt caataccgtt tacatggctt atctcgggca ggggagccat
    13561 gtgatctgcc acgaagccgt ctatggcccg tcgcgcgtgg ttctcgaaac gatctatacc
    13621 cggttcggcg tggaatatac aatggtcgac accaccgata tagaaaaagt gaaaaccgct
    13681 ttccgtccca acacaaaatt gcttttcacc gagactcccg ctaatccgac catggggatc
    13741 agcgacctgc cggcgctggc cgaactggcc cacagccaca atgtaccgct ggtggtcgac
    13801 aatactttct gcagcccgta tctccagaac ccgctggatc tgggtgccga catcgtcctc
    13861 cattcaatga ccaaattcat caacgggcat gccgatatcg tggccggaat gatcgttgcc
    13921 aaaaaagagg aagattataa gaaactccgg ggtattatga ccaacctggg ctgcaatatg
    13981 gacccccacc aggcttttat gacccggcgc gggctcaaaa cgctggcgat ccgtatcgac
    14041 cgggcgcagg aaaatgccat ggtcatcgcc ggatatcttg aaaaacaccc gaaagtggcc
    14101 tgggtaaaat atccgggact gaagtcgcat ccgcagtatg aacttggaaa aaggcaaatg
    14161 aaaggccccg gcgctatgat cagcttcgag ctgaaaggcg gcctcactgc aggcaaagtg
    14221 ctgatggaca atgtaaaggt gatgctgctg gccgtatcgc tcggtggaat tgaaaccctt
    14281 atccagcacc cggcctccat gacccattcc aaagtcagcg ccgaaaacag gcaccacgcc
    14341 gggattaccg acggactggt gcggctctcg gtaggtatcg aaaaagtgga tgacctgctg
    14401 gccgacctgg atcaggcgct tggcaaaata atctgaaata atgaccggaa caatccgcta
    14461 atttttcatc ttatttcatt cattttaaat aaatgctatt tttgcagacc ttaaaatttt
    14521 gagctatatc atccgtaaag gataacatct atttataatt aacacaacac agaaacctta
    14581 tctcactctt tagcctgaaa accagagagg tgagaacaca aacagtaatt ttgccatgac
    14641 ggaaaaagat ctgaccgctg ataacattca gcaggaactc cccgaaaaca cagaaatcac
    14701 ttccgaaccc tccgcaactc cggaagcgga aaccacggaa atcccagccg aagaaattct
    14761 taccgaacag gctgaaattc aggaaatcat cacagaaaca atcccaacgg aagaaatccc
    14821 ggttcaggac attgtgcccg atgactcacc gctggcggag gctttgacag acgatgttcc
    14881 ggttgaaatc tcgccagcgg aggtaattcc cgaagtaatt gctccggttg aagaaattcc
    14941 ggttgaagaa gttccgacgg agaatgcttc tgatccagaa cccgcagtcg aagatgtccc
    15001 ggtagaagag gttgctgctg aagatgttcc ggaagaaaag cctgttgcag aagatgcgga
    15061 gcccgaacct gttgctgcag aagagcagac tgcaaccgaa ctgcaccctg aagtaacagc
    15121 cgatgcagct ctgttgcagg agattgccga aatggatcat tccggtgaac aggagacaga
    15181 agaggaagaa gaagatatcc tggaagagca tgaggagatc gtagagagat acgataccta
    15241 cgaccgggaa aaactggtgg agatccttga agagaccgtc aaagaagaaa atattgtcag
    15301 gatcaagacc cgcgtggcca tgatcaaagt ggcgttcctc cgcctgtcga aggaatttcg
    15361 ccagcagttg ctcgacaacc atctgactgc aggagggacc cgggagaatt tcaccccgga
    15421 acccgatccg ctggttgaaa agttcaatgc cgtttttgag acatacaaga agaataaaat
    15481 aaaattcaat gagcaccagg aagtcgttaa actacgcaac ctggatgcaa aaaaagccat
    15541 cctggaggag ttgaaggagc tgataaattc ggaggaaaca ctcaaaaaga cctacgacca
    15601 gttcaaggaa ctccagacga aatggaaaga gatcggcctg gtgccttcca ccgaaatcaa
    15661 taacctctgg cagaattatc acttcctggt cgagaagttt ttcgacaaag taaagatcag
    15721 caaagagctt cgcgatctcg acctgaggaa gaacctggaa cataagatcg aactgtgtga
    15781 aaaagcggaa gagcttctca tcgagccatc catcattaaa tcgttccagc aactgcagaa
    15841 gtatcacgaa gagtggaaag agatcggacc ggtgccgcag gacaagcgcg acgagatctg
    15901 ggagcggttc aagacagcta ccgacaagat caacgaacgg cgcagagaac attacaacat
    15961 cctggccaaa gaccaggatg agaacctggc ggccaagaat gccctggtgg aaaaggcaga
    16021 acaactggct tcaattgagg tcgatgaact gaaaggctgg caggagaaaa cccgcgaagt
    16081 gaacgaactc ttccgccagt ggaagaccat tggccctgct cccaaaaaag agaacgacga
    16141 ggtatgggag aaattcaaag cttacctcaa cgctttcttc gccaataaaa aggagtactt
    16201 ctcccggatc aaacaggagc agctcaataa tttcaacctg aagctcgaca tcgtcaaaca
    16261 ggccgaagcg ctgaaagaca gcaccgattg gcgcaagact acccaggacc tgatcaattt
    16321 gcagaaagag tggaagaata tcggcccggt gcccaggaaa cattccgaca agatatggaa
    16381 acggttccgt gccgcctgcg acgaattctt caataataaa tccaaacatt tctccacagt
    16441 tgtcgaatcg gaaaaagata accttgacaa aaagctggcc ttgatcaagc aagtggaaga
    16501 gtgggaagcc gggaaggaca aatcggccaa cctggaagcc ctgaaaggat ttcagcggga
    16561 gttcatggag atcggtcatg tgccgatcaa ggagaagaac aaggtacacg atgcttttcg
    16621 ccaggctatc aacaagcatt tcgacaatct caagttaagc tcctacgaag gcaggcccca
    16681 gtcggaatac cgggaatacc gttccaggca ggattcttac gacggcgacc gttaccagag
    16741 acggggcggc agtaccggcg gcggcagcag cgaatggagt gccctgagca ccaaagtgag
    16801 caagttgaga gaggagatca tgctgtggga aaacaatatc ggtttcctgg cccattccaa
    16861 aaaagccgac ctgcttcggg ccgagttcga gaagaagatc caggatgcga aagcggagct
    16921 ggcctctctc gaagcaaagc tgaaatcgat gcaggagtga tcttgcatgt ccggtaattc
    16981 catcggtata tcgttcaaac tgactacatt cggcgaatcg cacggcccgg ctaccggtgg
    17041 cgtgatcgat ggctgcccgg ccggattgcc tattgacccc gaatttatcc aacaggagct
    17101 ggaccggcgg aaaccgttct acgaaggggc taccggcaga aaggaggccg atgccgtaga
    17161 attcctttcc ggcatcggga acggtatttc attgggaact cccatcgcct tcatcatacg
    17221 taatgaagat gttataacat cagattacca ggagattaaa gatgtttttc gcccctcgca
    17281 cgccgattac agctatttta tgaagtacgg caggatggca acaggagggg gacgtgcatc
    17341 gggacgtgaa acggccgcac gggttgccgg aggcgccgtg gctaagctac tgctgggaaa
    17401 ggccggtata tccgttaaag gcaaggtgca atcgatcggt gatggcacag ccgaaggcat
    17461 atccaaagca atagagcagg gcgatacgct cgggggtacg gtgacctgcc gcattgccgg
    17521 ggtacctgcg gggctgggcg agcctgtttt cgataagctc tccgccgccc tggccaaagc
    17581 ggcgatgagc attccatcgg ccaaggcctt tgaatacggc ctcggtttca tggccccgac
    17641 catgccaggt tcgaagtaca acgaccagat gaccgtaaaa gatggcaaag cggtgttcct
    17701 gacccatcac gacggaggca tccagggggg gatctccaac ggcgacgaca tatatttccg
    17761 tgtggctttc aaaccggtgc cgtcggttaa aattccccag gatacgatca ataaagattt
    17821 tgagccggtg cggattgaaa taggggggag gcatgatgtg tgccatgttc cgcggctggt
    17881 ggtggtggtt gagtcgatgg ccgccctggt gctggccgac cactggttgc gggcgatgaa
    17941 ctcgcggttg gcttaatctt tcagcattcc ctggatgttt tccacacggc cgtcgaccgg
    18001 cgccggttct aatccgagaa tttcacagat caaagggtaa agatggatgt gctcaaatgt
    18061 tggatgcctg tagttttgcc ggaaagcagg ccctctggcg aaaaagatgc catgcatatc
    18121 ggtattgtaa ggattgtacc catgggcacc tccggttcgg agaatcttat cggtcagcga
    18181 gaactgccat gccgagtcgg cgaccaggat aaaatcaagg gtgcgcggat tcgtgccgta
    18241 atgcaatgag tcgggcactt caccgtgctt ccagaccgta atgtgcggga tggccgacag
    18301 ggctttccag gcagtgtccc ggaattcctc cctgactttc agcgtcaggt tcggggtgta
    18361 tccttccatt tcttcaaacc agccgtaatc gagataattt ttcagcatga tgcagcgctc
    18421 cttgtgggta gggcccattc cgtggtcgga ggtaaagatg acgttgatgt cgccggcatg
    18481 cggcaaagtt tcgagtctgg ccataaatac acccacgagg ctgtcgaggg tggtgaccat
    18541 ccggcaggtg gcttcgctca aaggtccttc gctgtggcct gtgccatcag gttcgtcgaa
    18601 ataccacagt accaggtgcg gccgcttttc ttccggcagc gaaagccagt agatcaccgt
    18661 atcgatccgc tgtgaaaagg gaaattcgtg ctcatatttt ttccagtagg agggatggcg
    18721 tccctttgcc tccgacccga cccagaaaaa ggaagccgag gttattccct gcttttcggc
    18781 cgtcacccag atcggctcac cgccgtaaaa ggaggcgtcc tcaacggctg cccggttatt
    18841 gatgctgtag tgtttccccg tttgcggatc gaagaaactg ttctctacca gcccgtga
//
LOCUS       DAOPNH010000023        18755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2844_length_18755_cov_7.113583, whole genome shotgun sequence.
ACCESSION   DAOPNH010000023 DAOPNH010000000
VERSION     DAOPNH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2844_length_18755_cov_7.113583"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKZ74_02345"
     CDS             complement(<1..654)
                     /locus_tag="PKZ74_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM91620.1"
                     /translation="MRNSLQVRILSVMIIQVLLISGCNRPGNKEKPGKTIATETANIA
                     IPSFNADSALHFVKTQTGFGPRVPGSKAHADCASWLRSKLMDYTTDVRVQEFKTRVYS
                     GETFTGKNIIASFNPEKKIRIMLCAHWDSRPYADYDPDLAKHNLPVMGANDGASGVGV
                     LLEMARIFKNHPPDIGVDILFFDLEDYGPPKDSQNAANERHQHVGDARNRRSDRLCDL
                     "
     gene            complement(733..2073)
                     /locus_tag="PKZ74_02350"
     CDS             complement(733..2073)
                     /locus_tag="PKZ74_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPM91621.1"
                     /translation="MPRKKSKVAKIFVLDTSVILYNHNAIYSFEDNDIAVPITVLEEL
                     DNFKKGNDTKNFEAREFIRIMDRLSANHSLTNWIEIDQQGAGCIKVIMNENNSPIDAR
                     AVFDGNKPDHRILNAAMVMQDEHPQRPVILVTKDINLRLKAKSLDIQAEDYETGKIQD
                     VESLYPGKSTLEGVDKSVIDKLYSDGLVDPEDVGLTDPIPNHYFVLKNGNSSALAYFN
                     PLLGKIERVEKTQAYKITPRNAEQVFALHAIRNPRVKLVTLQGVAGTGKTLLALAGAL
                     DQKKNYKQIYLARPIVPLSNKDIGYLPGDIKDKLSPYMEPLYDNLKFIQNQYSESDKE
                     FKKITDALESEKLVITPLAYIRGRSISNVFFIVDEAQNLTPHEVKTIITRAGENTKIV
                     FTGDIYQIDTPYLDSQSNGLSYLIDKIKHHPIYAHVRLEKGERSELANLANDLL"
     gene            complement(2190..4172)
                     /locus_tag="PKZ74_02355"
     CDS             complement(2190..4172)
                     /locus_tag="PKZ74_02355"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPM91622.1"
                     /translation="MKQTTIHEFIVMLLIMTIAVNLQSCNKEPEPVGKLEILSCFAGE
                     TQLQLAAEVPNIALNTEIILNFSVPLDTNLAKQGISIKDLNNATFGVQYKFSGDMKTL
                     RLTHQQEFGKLKSYTVFIAGTVKGTEGESFTGAEYRFKTSDGKFNLIAATIDGQDFTT
                     NTKFYNIKRKNLRFSLEFSEPVDTAGISAKFSLSDDIKEIFEYGDNFRKVDLTLQGDL
                     EGYRKYTLVVSSGLESAYGNQFEGFSNSFFTEADSTYKFPVVTDDELLTIVQQQTFKF
                     FYDYAHPVSGMTRERLGSGDVVTSGGSGFGVMALIVGMERGFITRAEGMARLEKIIGF
                     LETCDRFHGAWSHWINGSNGYAIPFSTKDNGGDLVETGYMAQGLLTMRQYLDPLDPAE
                     SDLATRITELFNTIEWSWYTRGGQNVLYWHWSPTYNWDMNMKIQGYNETLITYILAAS
                     SPTYTIPAMAYKSGYARNGGIINGKTFYGYTLPVGYDYGGPLFFAHYSFLGLDPRNLS
                     DTYANYWTQNVNHSLINWAYCADNPKSFPNYSAHCWGLTAGDIPSGYGVSEPTNDRGV
                     ITPTAAVSSLPFSPEQSMDAIRFFYYILGDKLWGDYGFYDAFDVHVGWWAGSYIAIDQ
                     GPQVVMIENYRTGLLWDLFMSAPEVQAGLDKLGFSY"
     gene            complement(4357..5427)
                     /locus_tag="PKZ74_02360"
     CDS             complement(4357..5427)
                     /locus_tag="PKZ74_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM91623.1"
                     /translation="MKIIKSLFLLAMLTLVVASCEKELDPIIPVDPKPDQTAPTVAIS
                     YPIEGKAFVSPDEPATITFKVLAVDDIELQSVTLKLNGAEIVTFDSFLDYRRADIKYN
                     YSGMTAGTYTLSVDVVDLTGKTASTSVNFNKVTAPVYEPLAGEVLYFPLDGFNLDLVT
                     GAELAVNGAPGYAAGKVNDCYAGATDAYLTYPADAITSGTEFSVAFWYKINADPLRAG
                     ILAISPEGDSRNSGFRLFRENSGTNQNIGLNIGIGSTEVWMNPFVAVPADQDWMHIAI
                     VISTSKATIYVNGEIAPTNSEVDLESKIDWTGCSSMTIASGMPNFVYWEHFSDLSLYD
                     EMHFFTKALTPAEVLDLYTVKK"
     gene            complement(5457..6992)
                     /locus_tag="PKZ74_02365"
     CDS             complement(5457..6992)
                     /locus_tag="PKZ74_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM91624.1"
                     /translation="MKPYKFIIVISIALTVLLSNTGCNKYLDEPPENTTFTEETDYTI
                     SSNMVLPLIGAYERMNLVDIGWENFPLVAVRGDDVNAGGLGDQQDFAETDKYNYNKDY
                     WMYNSLWQNYYLSMFIANSAIEQVELYKPHASASLAEQYQAEARVLRSWWLFQLSRTF
                     GALPVPESSDPSELFVVPLSTKDEVMQMISAWMDEAIPLLPAVRPNERTDIPGGVTKF
                     TALAMKALANLELKNYQAVAEATGEIINSGKFELEADYYELFKIKGKLNSENLLEMQY
                     SDLGQAAGDNYYYLFAFFGPQGWTPAVEGAGDGWGFWEPSMKYIKFMLDRNEVTRLKT
                     TVLFTPRGIAEIQADPNYVNLPSWISNVTPSGDVINDYARAMFCSGKHYLPSIQLTPG
                     RTSYGTNKNFQCIRYAEILLMYAEALANGASGSAGTADAAVNAVRERAGLSPLAGVNL
                     QQVLDEKYAELAMEWGTRFFDMVRYNRVDALSYEGRTFSADKAFLPYPQNQVDQIPVL
                     KGN"
     gene            complement(7011..10061)
                     /locus_tag="PKZ74_02370"
     CDS             complement(7011..10061)
                     /locus_tag="PKZ74_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91625.1"
                     /translation="MKNYLTLKQAVGWLRSIFLLLLVVAGTTVFGQEKSISGSVTDRS
                     TNESLPGASVLVKGTGAGTITDIDGKFALKVPPGGTSIVVSYVGYETQEITIGSQAVF
                     NVMLSPSKTSLQEVVVVGYGSTKVKDLTSSISTIKAEDINRTPASQPLQALQGKVPGL
                     QVVTSGGPGSMPTIRIRGIGSFPGRENEAPLFVVDGMFFDNIDFLNPSDIASISVMKD
                     ASAAAIYGVRAANGVVLIETKSGQYNQKAEITYNGYGGYQIAQNVVKMANSQQFSIMA
                     RESGSPADETFILNAMQRYGRSRVDPSIPEPNTNWYAEILRPAAITNHSLDFSGGTEK
                     ARYSLGANYFYQDGIMNMDNNYERLNLRTKLDFKATNWLTVGGNMVYSNALKNNEQNS
                     AWNNAYFAVPIMPVYDTLNNLAYPDNYSSARIMGYRSGQNPFPYMVYNFDQMKIKKLL
                     SNFYVEINLLPKVLTFKTSYNYSYTGIDQRIINFPYYIDDGFNNLDPKITRTWTNYNN
                     HIWDNILTFTKSFQNHSLTLMAGTSFKDEGYTMLSAWVNDIPTDKPQSWYINKTLDIE
                     SIGATDGGLRQYGMSYFGRLAYNFNNRYLLYATMRADGSNKYQQTWGYFPTVGIGWVV
                     TEEKFMPKNDILTYLKIRASWGQLGNDKIQASDGAFTTTVIKTTFGGVVYSGTVVSSD
                     YSELKWELTEETNVGFTAKFVKNKLSADIDYYNRDTKNAAIPVSIPSVGGSVLKPVGT
                     IRNSGIEIALNWSNEINGKLNYSVGANLATLKNQAIDLYGQEYIDGGTAEFRQRTYVG
                     EPLMAFYGWEVAGVYQNATEISNDPIAVDNNLVPGDFKYVDQNKDGLLDGDDRVILGS
                     YFPNLIYGGDISIRYLNIDFSASFYGQAGNKILNRKRGELIWTSDGNLDADLAENRWY
                     GEGTSDKYPSSSGLRRGWNQKMSDYFVEDGSFFRFQNIALGYTIKNSKWLGGNFPETR
                     ISFTAERPLSLFKYNGFTPEVANGWDSQTYPVAAIYTVGLNIKF"
     gene            10266..13103
                     /gene="polA"
                     /locus_tag="PKZ74_02375"
     CDS             10266..13103
                     /gene="polA"
                     /locus_tag="PKZ74_02375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPM91626.1"
                     /translation="MEMNNRLFLLDAMALIYRAYFALNKNPRINSKGLNTSAILGFAN
                     TLYDLVKNEKPTHIGVAFDSFAPTLREAEYTEYKAQREKMPEDIALALPYIRRLLEGF
                     RIPILELPGYEADDIIGTLAHKADKKGFTTFMVTSDKDFGQLVSDRTFIYRPPRFGNK
                     AEILGIKEVCERYQIERPAQLIDILGLWGDASDNIPGIPNIGEKRAIELIKQYGSVEN
                     LIAHSGELQGKIRENVEAFAEQGLFSKKLATIILDVPLEFEEEKLILERPSFEILNEL
                     FNELEFRTFAKRVFTDLSVREQAGEEVTGLVAQAGGGKPQAGGDLFSELPATEKGTPS
                     LGTPSQGMASLDKTRVNYHLVTSKDQRQQLVDKLKHAEKFCFDTETTGIDPNNSELVG
                     ISFCFQPFEAFFVHLPANYQECLEIVSEFKPFLENENVLKIGQNLKFDIAILKWYEVE
                     VRGKLFDTMLAHYLIEPDLKHGMDFLSETYLNYKTVHIDELIGKKGKDQGNMRDVQPE
                     ILSDYACEDADITYRLYEIFDPMLNEGGLRKLFEEIEIPLVPVLAAMEAEGVMLDTKA
                     LKDYSAHLGDEVHEVEEQIYDLAGQLFNIASPKQLGEILYEKLKITDKPPRTATKQYS
                     TSEDVLVKMLFRHPIIQLILDYRSLTKLKSTYVDVLPTLISPRDKRIHTSYNQAVAAT
                     GRLSSNNPNLQNIPIRTDRGKEIRKAFVPRNPDFILLSADYSQIELRIIAHMSGDKNM
                     IESFRQGTDIHAATAAKVYGIQVGEVTPEMRRKAKAVNFGIIYGQSAFGLAERLNITR
                     REAAEIIESYFAQYSGIREYMDRTIDFARKHGYVETILGRRRYLRDINSANAVVRGFA
                     ERNAINAPIQGSSADMIKIAMIRIHEDLKKLATRSRMILQVHDELVFDVHRSERELIR
                     PVVYEQMKNALPLDVPVEVEMDEGENWLEAH"
     gene            13146..14252
                     /locus_tag="PKZ74_02380"
     CDS             13146..14252
                     /locus_tag="PKZ74_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPM91627.1"
                     /translation="MNLTTDRRIVMTLDAGGTNFVFNAIRGGEEMFEPFRLKAKGATL
                     EEVLLKIIDGFNNVKSALDEKPSAISFCFPGPADYPRGIIGDLENLPTFRGGVALKDM
                     LEEKFGIPVFINNDGDLFAYGEAIGGILPEINARLEAAGNPRRYRNLFGVTFGTGFGG
                     GIVNNGQLFIGDNSAGGEVNRMRNRLYLSTSAEDSTAIRGVRRVYARETGIPFEESPN
                     PKEIQDIILGSFPGNKEAALKAWEEMAVVAGDTLANAITLIDGIIVIGGGLSGAHGVF
                     LQRLVDEMNASFTSLDGHEVPRLEITALNLENPVELAEFLENKSIEIKVPFSQKTVNY
                     NPARYIGVGVSRLGTSRAVSVGAYAYALAELDKI"
     gene            14418..15248
                     /locus_tag="PKZ74_02385"
     CDS             14418..15248
                     /locus_tag="PKZ74_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM91628.1"
                     /translation="MKLYSIETGNLKLDGGAMFGVVPKSLWSKVYPADENNLCNWAMR
                     CLLVVDEDRRILIDNGIGDKQDWNFLRHYHLNGDDTLEKSLAKAGFGKEDITDVILTH
                     LHFDHCGGSVEWNHDRTGYKLAFPNANYWVSRQQYNWAVEPNHRESASYLKENILPIE
                     ESGKLKLIEGEGEILPNITIKLYHGHTDGQAIPHIRYNGKTVVFMADLLPSTAHVPMP
                     WIMGYDTRPLITLEDKTRFYREALENDYILFLEHDLYHECCTLHQTDKGVKVKETFEL
                     "
     gene            complement(15256..17454)
                     /locus_tag="PKZ74_02390"
     CDS             complement(15256..17454)
                     /locus_tag="PKZ74_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM91629.1"
                     /translation="MKVFQTNEIRNIALIGGAKTGKTTLAEDMLFEGGVINRRGSIED
                     KNTVSDYRDIEMERQNSVYSSVLYSIYNNHKINVIDTPGFDDFVGEVISAMKVVDAAV
                     SVVNAQNGVEPGTETTWRLCTKELMPVIFAINHLEHDNSNFDDTVSQLKSSFSSAVTV
                     VQYPLHTGHGFNSVIDLLKMKMLKYPDGGGKPEILDIPANEKGKAEELQAKLIEDLAA
                     NDESLMESFFENGTLSEEEISRGLRLGLRNRGIFPVLCVNAKQNMGVGRLLEFIINNV
                     SAPNEMPPVKTVSGKEVKCLATEPAMAFVFKTTIEQHLGEVSFFKVFSGEIHEAEDMI
                     NGTNATKERLSQVFVFAGKNRDKVEKMVAGDIGATIKLKNTLTNHTLNAPKFADEKIA
                     PIEYPDSKFRVACKAVNQADDEKLGMALNELHKMDQTIIAGYKKELRQLILEGQGELH
                     LNIAKWHLENNIKIQVNYLPPRIPYRETITRVAQATYQHKKQSGGAGQFGEVHMVIEP
                     HEEGKPDPSSVKLKQMMRQSSVTPLTISKGDTEISLSIRGKDEIQLEWGGKLVFYNCI
                     VGGSIDTRFLPAILKGLMEKMEQGPLTGSYARDIRVYVYDGKMHPVDSNEISFKLAGR
                     TAFSMAFKEASPKILEPIYDVEVFVPADKMGGVMTDLQGRRAIIMGMDGGTIRARVPL
                     AELNRYSTALSSLTSGRASYTMKFNEYAPVPSDIQEKLLKEYEESAKDED"
     gene            17653..>18755
                     /locus_tag="PKZ74_02395"
     CDS             17653..>18755
                     /locus_tag="PKZ74_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91630.1"
                     /translation="MIRKALPFLVILFFSITLAGQEIPEAYRNYNAKYPSGPVMTNRD
                     AALLRSLPEKKVPETAVRDQLPAIVDNSTLPYLRPVFLQEGPSCGQAAMVGYNFTYEM
                     ACKRDQPALFPQTQYPTHFTWNFQNGGDGWYGVSYFHSVEILRMCGCMNSYDYGGFFD
                     DGRRWINGYNLYNNGMYNRVRQMYRINTSTEEGILALKHWLYNHMGEQEHGGVASYYA
                     NVPWNAVFLNDTTPEGGKYVVTWWYPLASHAMTIVGYNDSIRYDYNGDGQYTNTIDLN
                     NDQVIDPRDWEFGGVKFVNSHGTGSQDSGFCYMMYKVLAEKFETGGIWNQEVHILDVD
                     EDYLPLMAFKVKIKHTNRGKIKVMAGVSQDTSD"
BASE COUNT         4538 a         4581 c         4830 g         4806 t
ORIGIN      
        1 aagatcgcat agtcggtcgc tgcggcgatt gcgagcgtca ccaacatgct ggtgacgctc
       61 attggccgcg ttctggctgt ctttcggcgg gccatagtct tccaggtcga aaaagaggat
      121 atcgaccccg atgtcgggcg gatgattttt aaagatccgg gccatttcaa gtaaaacacc
      181 cacaccgctg gcaccgtcgt tggctcccat caccggcagg ttgtgtttgg ccaggtccgg
      241 atcataatcg gcataaggcc ttgaatccca gtgtgcacag agcataatcc ggattttctt
      301 ctccgggttg aatgaggcga tgatattctt ccctgtaaag gtctccccgg aataaacccg
      361 tgttttaaac tcctggaccc tgacatccgt ggtataatcc atcaatttgg atctcagcca
      421 ggaagcgcaa tctgcatggg ctttgcttcc cgggacacga ggcccgaacc cggtttgtgt
      481 tttgacaaaa tgaagagctg aatcggcatt aaaagaaggg atagcaatgt ttgctgtttc
      541 agtggcaatt gtttttccag gtttttcttt attgccgggc ctgttacagc cggatatcaa
      601 tagtacctgg atgatcatta ctgacaggat tctgacttgt aggctatttc tcacagataa
      661 tcacagatta aagacacaga aaacacagaa tatttcccaa acttaacacg tacgactcaa
      721 accttaacat ttttacagca ggtcattcgc caggttggcc agttcgctcc gttcgccttt
      781 ttcgagcctc acatgggcat agatcgggtg gtgcttgatc ttatcgatga ggtacgacaa
      841 tccattactc tgcgaatcaa ggtatggggt gtcgatttgg tagatatcgc cggtaaagac
      901 gatcttggtg ttttcgccgg cgcgggttat aatcgttttc acctcgtgag gggtgaggtt
      961 ctgcgcctca tccacgataa agaacacatt cgagatgctt ctaccacgga tataggcgag
     1021 aggcgttatc accagctttt cgctttcgag cgcatcggtg atctttttga actccttatc
     1081 gctttcgctg tactggttct gaataaactt caggttatcg taaagcggct ccatgtaggg
     1141 actgagcttg tctttgatat ctcccgggag gtaaccgata tctttattgc tcagcgggac
     1201 tatagggcgt gccaggtata tctgcttgta atttttcttc tggtcgagcg cgccggccag
     1261 ggcgagaagg gtctttcccg taccggcgac tccctgtagc gtaaccagtt taacacgcgg
     1321 attacggata gcgtggaggg caaatacctg ttcggcattt cgcggggtga ttttgtaggc
     1381 ctgcgttttt tccaccctct ctatttttcc aaggagcggg ttgaaatagg ccagggccga
     1441 ggagttgccg tttttcagta caaaatagtg gttcgggatg ggatcggtaa gccccacatc
     1501 ttcgggatca accagaccat cggagtacag tttgtcgatc acgcttttgt cgacgccttc
     1561 cagggtcgat ttgcccggat agagcgattc gacgtcctgg atcttgccag tttcataatc
     1621 ctctgcctgg atatcaagtg atttggcctt aaggcgaagg ttgatatctt ttgtcacgag
     1681 gatgaccgga cgttgcggat gttcatcctg catgaccatg gcggcattca ggatccggtg
     1741 gtcgggctta ttgccgtcga aaaccgcccg ggcgtcgatc gggctgttgt tctcattcat
     1801 gatcaccttg atacaaccgg ctccctgctg atcgatctca atccagttgg tcagcgaatg
     1861 attggcagag aggcggtcca taatacggat gaactcacgg gcctcaaagt ttttggtatc
     1921 gttccctttc ttgaaattat cgagctcttc cagcacagtg attggaacgg caatatcatt
     1981 atcttcaaaa ctataaatgg cattatggtt ataaagaatg accgacgtat cgaggacgaa
     2041 tattttagcg actttacttt tctttctggg catctatgga gagttaattc taaattttaa
     2101 attttaaatt cttaattctt aattcataat gataatggga tgtaaaaata gtcaataagg
     2161 aaatttgtca ttttgaatat ttatcaacac taatagctga agcccagttt atcgaggccg
     2221 gcctggactt cgggggcgct cataaagaga tcccagagaa gtccggtacg gtaattttcg
     2281 atcatgacga cctggggtcc ctggtcgatc gcgatatagg agccggccca ccagcccaca
     2341 tggacatcga aggcatcata gaatccataa tcgccccaga gtttatctcc gaggatataa
     2401 tagaagaacc ggatagcgtc catcgattgt tccggagaaa aagggagaga ggagaccgcc
     2461 gcggtcgggg tgatcacgcc ccggtcgttg gtgggttcgc tcacgccgta tccggaaggg
     2521 atatcgccgg cggtcaatcc ccagcaatgt gcgctgtaat tgggaaagct tttcggattg
     2581 tcggcacaat aggcccagtt gatcagggag tggttgacat tctgcgtcca gtaattggca
     2641 taggtatccg aaagatttct tggatcgagc ccgaggaacg aatagtgggc aaagaaaagc
     2701 ggcccgccgt agtcataacc tacggggagg gtatagccgt agaacgtttt gccgttgatg
     2761 atccctccgt tgcgggcgta gcctgactta taggccatag cggggatggt gtaggtcggc
     2821 gaggaagcgg cgaggatgta agtgatcagt gtctcgttgt agccctggat tttcatgttc
     2881 atatcccagt tataggttgg cgaccaatgc cagtaaagaa cattttgccc gccgcgggta
     2941 taccagctcc actcgatcgt gttgaaaagt tcagtgattc ttgttgccag atcactttcc
     3001 gccggatcca gcggatcgag gtactgtcgc atagtaagga gcccctgggc catgtatccg
     3061 gtttcgacca ggtcgccgcc gttgtctttg gtgctgaacg ggatagcata gccgttgctg
     3121 ccgttgatcc agtgcgacca ggctccgtgg aaccggtcgc aggtttccag gaatccgatg
     3181 atcttttcca gccgggccat accttcggcc cgggtgataa atcctctctc catgccgacg
     3241 ataagcgcca tgaccccgaa cccggagccg ccagaagtaa caacatctcc cgagccgagt
     3301 ctttccctgg tcattccgct caccggatgg gcataatcat aaaagaactt gaacgtttgc
     3361 tgctggacga tggtcagcaa ctcgtcatcc gttactaccg ggaacttgta ggtggagtcg
     3421 gcttcggtaa agaaagaatt gctgaacccc tcgaactggt ttccgtaggc tgattcgagg
     3481 cctgatgaga ctaccagggt atatttgcgg tatccttcca gatcaccctg aagtgtcagg
     3541 tcgaccttcc ggaaattgtc cccgtattca aaaatttctt taatgtcgtc tgaaagggag
     3601 aacttcgctg aaattccggc ggtatcaacc ggttctgaaa attccagcga aaaccggagg
     3661 ttttttcttt ttatattgta aaacttggta ttagtcgtaa aatcctgacc atcaattgtt
     3721 gcagctatca aattaaactt cccgtctgat gttttaaacc tgtattccgc tcctgtaaac
     3781 gattcgcctt ctgttccttt cactgtgccg gcgatgaaaa ccgtgtaact tttcagtttt
     3841 ccgaattcct gctgatgggt gagccgtaat gttttcatgt ccccggaaaa cttatattgc
     3901 accccgaagg tggcgttatt caaatctttg atggagatgc cctgtttagc caggttggta
     3961 tcaaggggta ccgaaaagtt caggatgatt tcggtattca gggcaatatt ggggacctcg
     4021 gcagccagct gaagctgggt ttcaccggca aaacaggaga ggatctcgag tttcccgacc
     4081 ggctcgggtt ctttgttgca ggattgaaga ttaactgcga ttgtcatgat caataacatg
     4141 acaataaatt catgtattgt ggtttgtttc atctttataa aattattaca tccgcaatta
     4201 attcatccgg gtcattaaaa attacttaac cgcaaagtta cacaaaggga ttcacaaagg
     4261 cgcacaaagt aattgattac caaatatcaa actttgtacg cctttgtggt taaagaacct
     4321 taaaatgact tatagcgaca gaattgaata ccgactctat tttttaacgg tatagagatc
     4381 cagtacctca gccggggtca gggcctttgt gaagaaatgc atctcatcat agaggctcag
     4441 gtcggagaaa tgctcccagt agacgaagtt cggcatccct gaagcgatgg tcatactgct
     4501 gcatccggtc cagtcgattt tggattccag gtccacttcg gagttcgtcg gggcgatctc
     4561 gccgttgacg tagatagtgg ctttcgaggt cgagatcacg atggcgatat gcatccagtc
     4621 ctggtcggca ggaaccgcga caaacgggtt catccagacc tcggtggaac cgataccgat
     4681 attcagtccg atattctggt tggtgccgct attttcgcgg aacaacctga agccgctgtt
     4741 ccggctgtcg ccttcggggc tgatggccag aatcccggca cgaagcggat cggcattgat
     4801 cttataccag aaagccacgc tgaattctgt tcccgaggta atcgcatcag ccgggtaggt
     4861 caggtatgcg tcggtagccc cggcataaca gtcgttcact tttccggctg catatcccgg
     4921 agcgccgtta accgccagtt cagcgccggt aaccaggtcg aggttgaagc catcgagcgg
     4981 gaaatagagc acttcgccgg cgaggggttc ataaaccggt gcggtcactt tgttaaaatt
     5041 aaccgatgtc gacgccgttt tcccggtaag gtcgaccaca tcaaccgaca gggtataagt
     5101 acctgcagtc ataccgctgt aattgtattt gatatcggct cttctgtaat ccaggaaaga
     5161 gtcaaatgtt acgatctccg cgccgttaag cttcaatgta accgattgga gttctatatc
     5221 atccactgcc agaaccttaa atgtaatggt agcgggttca tccggtgata cgaaggcttt
     5281 accttctatc gggtagctga tggcaaccgt tggcgcggtt tgatccggtt tcggatcgac
     5341 cgggatgatc gggtcgagtt ccttttcaca ggaggccacg accagggtta acatcgccag
     5401 taaaaacaag gattttataa ttttcatctt gaattttttt aaagttaaac cataggttaa
     5461 ttacctttta ataccggtat ctggtcgacc tggttttgcg gataaggcag aaatgcttta
     5521 tcagctgaaa atgttcttcc ctcgtagctc agtgcatcaa ctctattgta ccggaccata
     5581 tcgaagaacc gggttcccca ttccatagcc agctcggcat atttttcgtc gagcacctgc
     5641 tggaggttca ctccggccag cgggctcaaa cctgctcttt ccctgacggc attcactgct
     5701 gcatcggctg ttccggcgct gccggatgcc ccattggcca atgcctcggc atacatcagc
     5761 aggatctcgg catagcggat acactggaag ttcttgttag taccatagct tgttcttccg
     5821 ggggtaagtt ggatggaagg caggtaatgc ttgccgctgc agaacattgc acgggcgtag
     5881 tcgttgataa catcacccga aggggtcaca tttgaaatcc aggaaggcag gttcacatag
     5941 ttcggatccg cctgtatctc ggcaattccc ctgggggtga acagaaccgt cgtcttcagg
     6001 cgggttactt cattccggtc gagcataaac tttatatatt tcatgctcgg ttcccagaag
     6061 ccccatccgt cgccggcgcc ttccaccgcc ggtgtccatc cctgcggtcc aaagaatgca
     6121 aacaggtaat aatagttatc gccggctgcc tggcccaggt cggaatactg catttcgagc
     6181 aggttctcgc tgttcagctt tcctttgatc ttgaacaatt catagtaatc ggcttcgagt
     6241 tcaaattttc ccgaattgat gatctcgccg gtggcttcgg ccacagcctg gtaattcttc
     6301 agctccaggt tggccagggc tttcatggcg agggccgtga attttgtgac gccgcccggg
     6361 atatcggtac gttcgttggg cctgacggca ggcagcagcg gtatggcttc atccatccag
     6421 gcagagatca tttgcatgac ctcatccttt gttgataggg gaaccacgaa gagttccgac
     6481 gggtcggagc tttcgggcac cggcagtgcg ccaaatgtac ggctgagctg gaacagccac
     6541 catgaccgca gcaccctggc ttcggcctgg tattgttcag ccaggctggc gctggcatgc
     6601 ggcttgtaaa gctctacctg ttcgatcgcc gaattggcga tgaacatgct caggtagtaa
     6661 ttctgccaca gggagttata catccagtaa tccttgttgt agttgtattt gtcggtttcg
     6721 gcaaaatcct gctggtcgcc caggccgccg gcattaacat catcgccgcg cactgcgacc
     6781 aggggaaaat tctcccagcc gatatcgacc aggttcatcc gctcataggc accgattaaa
     6841 ggaaggacca tgttgctgct gatggtgtag tcggtttctt cggtgaacgt cgtattttct
     6901 ggcggttcat cgaggtactt gttgcatcct gtgtttgaca acaggacggt cagcgctatt
     6961 gaaataacta taatgaattt atatggtttc atatcaataa gtatttagcg ttagaacttg
     7021 atattgagtc cgaccgtata gatcgcggcc acgggatagg tctgagagtc ccatccgttg
     7081 gcgacttccg gtgtgaagcc gttgtatttg aagagcgaca gcgggcgttc ggcggtaaat
     7141 gagatccggg tttccgggaa attgcctccc agccatttgc tgtttttgat ggtatagccc
     7201 agggcgatat tctggaaccg gaagaaagag ccgtcttcca cgaaatagtc gctcattttc
     7261 tggttccagc ccctgcggag gcctgacgat gacgggtact tatcggaggt gccttctccg
     7321 taccagcggt tttcggccag gtcggcgtcg aggttgccgt cggaggtcca gatcagctct
     7381 ccccttttcc ggttgaggat tttgtttccg gcctgaccgt agaaactggc gctgaagtcg
     7441 atgttaaggt aacggatact gatatcgccg ccatagatga ggttcgggaa gtaggaaccg
     7501 aggatcacgc ggtcgtcgcc atccagcaaa ccatctttat tctggtcgac atatttgaag
     7561 tcaccgggga cgagattatt gtcgaccgca atcggatcgt tgctgatttc agtcgcattc
     7621 tggtaaacgc cggccacttc ccatccgtag aaagccataa gcggttcgcc gacgtaggtg
     7681 cgctgacgga attcagcggt accgccgtcg atatactcct gtccgtaaag gtcgatggcc
     7741 tggtttttca gggtagccag gtttgccccg acgctgtagt tcagtttgcc gttgatctca
     7801 ttgctccagt tcagtgcgat ttcgataccg gagttgcgga tcgtaccgac cggtttcagc
     7861 acgctaccac cgaccgacgg gatagagacg gggatggcgg catttttcgt atcgcggtta
     7921 tagtaatcaa tatcagccga taatttgttc ttcacaaatt ttgcggtgaa accgacatta
     7981 gtttcctcgg taagttccca tttcagctca gaataatcgc ttgacaccac agtaccggaa
     8041 tagacgacgc caccgaaggt ggtttttata acggtagtgg taaacgcgcc atcgctggcc
     8101 tggatcttgt cgttgcccag ttgcccccag ctcgctctga tcttcagata agtaagaata
     8161 tcgtttttcg gcatgaactt ttcttcggtg acgacccagc caataccgac cgtggggaaa
     8221 tagccccagg tttgctggta tttgttgctg ccgtcggcac gcatcgttgc gtagagcagg
     8281 tagcggttgt tgaagttgta ggccagacgt ccgaagtaag acattccgta ttggcggagg
     8341 ccaccgtcgg tggcgccaat ggattcgata tccagggttt tattgatata ccaggattgc
     8401 ggtttatcgg tcgggatatc gttgacccag gcgctcagca ttgtgtagcc ctcgtccttg
     8461 aaagaagttc cggccatcaa tgtcaggcta tgattctgga agctcttggt gaatgtcagg
     8521 atattatccc agatatggtt gttataattg gtccaggtgc gggtaatttt cgggtccagg
     8581 ttattgaaac catcatcaat ataatacggg aaattgatga tacgttgatc gatcccggta
     8641 tagctgtaat tgtatgatgt tttaaaagtg agaaccttcg ggagaagatt gatttcgaca
     8701 tagaaattgg ataacaactt tttgattttc atctggtcaa aattgtatac catatacggg
     8761 aagggattct gtcccgatct gtaacccatt atgcgtgcac tggagtaatt atccggataa
     8821 gccaggttgt tcaaggtatc gtaaacgggc atgatcggga cggcgaagta ggcattgttc
     8881 catgccgaat tttgctcatt gtttttcaat gcattgctat ataccatatt accgccaact
     8941 gtcagccagt ttgtggcctt aaaatcgagt ttggtccgga ggttaagacg ttcgtagtta
     9001 ttgtccatat tcatgatccc atcctggtag aagtagttgg cgccgaggga atagcgtgct
     9061 ttctccgtac cgcctgagaa atccaggcta tggttagtga tggcggccgg gcggaggatt
     9121 tcggcgtacc agttggtatt gggttccggt atactgggat ctacgcggct tctgccgtag
     9181 cgttgcatgg cgttaaggat gaacgtttca tcggcgggtg agcccgattc cctggccatt
     9241 atggaaaact gctgggagtt ggccatcttc accacattct gggcgatctg gtaaccgcca
     9301 taaccattgt aggtgatttc agccttctgg ttgtattgtc ctgattttgt ttcgatgagt
     9361 acaacgccgt tggcggccct gacaccatat atagcggctg ccgaggcgtc tttcatgaca
     9421 gaaatggagg cgatgtcgga aggattcaga aagtcgatat tatcgaagaa catgccgtcg
     9481 acgacaaaca gcggcgcttc attttcccgt ccggggaatg agccgatacc gcggatacgg
     9541 atagtgggca tactgccggg gcctccggaa gtgacgacct gcagaccggg gacctttccc
     9601 tgcagggcct gcagcggttg agaagcggga gtccggttaa tatcttccgc tttgatggta
     9661 ctgatggaag aggtcaggtc tttcactttt gtgcttccgt atcctacgac cactacttcc
     9721 tgcaaagagg ttttcgaagg ggatagcatg acattgaaaa cggcctggct tccgatcgtt
     9781 atctcctggg tttcgtagcc cacatagcta acgacaatgg atgttccgcc gggaggcacc
     9841 ttcaatgcga atttaccgtc gatatcggta atcgtaccgg ctccggtacc ttttaccaat
     9901 accgatgctc cgggcaggga ttcgttcgtt gaccggtcgg taaccgagcc gctgatgctc
     9961 ttctcctggc cgaacacagt ggttcctgca acgaccagca agagcagaaa aatgctcctt
    10021 aaccagccga cagcctgttt aagtgtaagg taattcttca taacatattg attttggtat
    10081 tagttaagaa atattttcgg gtttgtggaa gtcataccgg gtaaaatcaa tccttcttac
    10141 aaatataata ataatggtgt tttcggaaaa tgtgaaaata ttatgtttaa tcgtgtttgg
    10201 gggtccatac gggattatca ggatgcggtt ttgaatttcc tttgattatc tttgctcaat
    10261 actcactgga aatgaataac cgcctctttt tattagatgc tatggccctg atttacaggg
    10321 cttatttcgc cctgaataaa aatcccagga tcaattcaaa aggattgaat acctccgcta
    10381 ttcttggttt tgcgaatact ttatatgatc ttgtcaagaa tgaaaagccg acccatatcg
    10441 gggtggcttt tgattcgttt gcgccgacgc tccgcgaggc agagtacacc gaatacaaag
    10501 cccagcggga gaagatgccg gaagatattg ccctggcgtt gccctatatc cgtcgtttgc
    10561 tggagggatt ccgtatcccc atcctcgagc tgcccggtta tgaggcggat gatattatag
    10621 gaacgctcgc acacaaagca gataaaaagg gatttacgac gttcatggtg acgtccgaca
    10681 aggattttgg gcagcttgtc agcgacagga cctttattta ccgtccgccc aggtttggca
    10741 acaaagcgga gattttaggg ataaaggaag tgtgtgaaag ataccagatt gaaaggccgg
    10801 cgcaattgat tgatatttta ggattatggg gcgatgcgtc cgataatatc ccggggatac
    10861 cgaacatcgg ggaaaagagg gccatagagc tgataaagca atacggatcg gtggagaacc
    10921 tgattgccca ttccggcgag ttgcagggaa agatcaggga aaatgtcgaa gcctttgcag
    10981 agcaggggct tttctcgaag aagctggcca ccataattct ggatgtgcct ttggaatttg
    11041 aagaggagaa gctgatactg gagagaccct ctttcgagat cctcaacgaa ttgttcaatg
    11101 agctggaatt caggactttc gcaaagaggg tattcactga cctttcggta agggagcagg
    11161 ctggagaaga ggtaaccggg ttggtggccc aggccggcgg ggggaaaccg caggctgggg
    11221 gagatctttt cagcgaattg cccgccaccg agaaagggac gccatccctt gggacgccat
    11281 cccaagggat ggcgtccctg gacaagaccc gggtcaatta ccatctcgtc acttccaaag
    11341 accagcggca gcaactggtg gacaaactca aacatgccga aaagttctgc ttcgataccg
    11401 aaaccaccgg catcgatccg aacaactcgg aactcgttgg tatctcattc tgcttccagc
    11461 cctttgaagc cttttttgtg cacctcccgg ccaattacca ggaatgcctc gaaattgtca
    11521 gcgagttcaa acccttcctg gaaaacgaaa acgttctcaa gatcggacaa aacctcaaat
    11581 tcgatatcgc tatcctcaaa tggtatgagg tggaggtacg cggcaaattg ttcgacacga
    11641 tgctggccca ctacctgatc gaaccggatc tgaaacacgg aatggatttt ctttccgaaa
    11701 cataccttaa ttacaaaacc gtccatatcg atgaactgat cggcaagaaa ggcaaagacc
    11761 aggggaatat gcgggatgtg caaccggaaa tcctttccga ttacgcctgc gaagatgccg
    11821 acatcaccta ccgcctctat gaaatcttcg atccgatgct gaatgaaggc ggcctcagaa
    11881 agcttttcga agagatagaa attccgctgg tgcctgtcct ggccgccatg gaggcggaag
    11941 gcgtaatgct cgacacgaag gctttgaaag attattccgc acacctgggc gatgaggtcc
    12001 atgaggtgga ggagcaaatc tacgacctgg ccgggcaact ctttaatatc gcatcgccca
    12061 aacaactcgg cgaaattctt tatgaaaagc ttaaaatcac cgataagccg ccgcgcacgg
    12121 ctaccaaaca atattcgacc agcgaggatg tgctggtcaa gatgctgttt cgccatccga
    12181 tcatccagct gatcctggac taccgttcac tgaccaagct taaatcgacc tacgtcgatg
    12241 tgttgcccac cctgatcagc ccgcgcgaca aacggatcca cacctcttac aaccaggcgg
    12301 tggctgcaac aggccggctc agttccaaca atccgaacct gcagaatatc ccgatccgga
    12361 ccgaccgggg aaaagagatc cgaaaagctt ttgtaccccg aaatcccgat ttcatccttt
    12421 tatcggccga ttactcccag attgagttgc ggatcattgc acatatgagc ggagataaaa
    12481 acatgatcga atctttccgg cagggaaccg acattcacgc cgccacagcc gccaaagtct
    12541 acggtatcca ggtcggcgaa gtcaccccgg agatgcgccg gaaagccaag gcggtcaatt
    12601 tcggtatcat ttacgggcag tcggcgttcg gactggccga acggctgaac attacccgcc
    12661 gcgaagcggc agagatcatc gaatcctact tcgcacagta ttcggggatt cgggaatata
    12721 tggaccgcac catcgatttt gcccggaagc acgggtatgt ggagaccatc ctgggaagaa
    12781 ggcggtatct gcgcgatatc aattcggcca atgccgttgt gcgcgggttt gctgaaagaa
    12841 acgccatcaa tgccccgatc cagggttcat cggccgatat gatcaagatc gccatgatcc
    12901 gcatccatga agaccttaaa aagcttgcaa ctcgcagccg gatgatcctg caggtccatg
    12961 acgaactcgt tttcgacgta cacagatcgg aaagagaact gatccggccg gttgtgtatg
    13021 agcagatgaa aaatgcactg ccgctggatg taccggtgga ggttgagatg gatgaagggg
    13081 aaaactggct ggaggcgcat tgaaataata tttttctatt tttgatcatt ccaaattcca
    13141 agattatgaa cctgaccacc gacagacgca ttgtcatgac cctggatgcc gggggaacca
    13201 actttgtatt caacgccatt cggggcgggg aagagatgtt tgaacctttc cgcctgaaag
    13261 caaaaggagc tacactggaa gaggtgctgc tcaaaataat cgatggtttt aataatgtaa
    13321 aaagcgcact ggatgagaaa ccgtcggcca tcagcttctg ttttcccggt ccggccgact
    13381 atccgcgggg catcatcggc gatctggaga atctgcctac attcaggggt ggagtcgcac
    13441 tgaaagacat gctggaagag aaattcggca tcccggtgtt cattaacaac gacggtgatc
    13501 tgtttgccta cggtgaagcc atcgggggca tcctgccgga gatcaatgcc cgtttggagg
    13561 cggccggtaa tcccaggcga taccgcaacc tgttcggcgt tactttcgga accggtttcg
    13621 ggggaggcat tgtcaacaac gggcagctat tcatcggcga caattcggcc ggcggtgaag
    13681 taaaccgcat gcgcaacagg ctctatctct ccaccagcgc tgaagacagc accgccatcc
    13741 ggggtgtccg ccgggtatat gcccgcgaaa caggcatccc gtttgaggaa tctccaaatc
    13801 ccaaagagat ccaggatatt attctcgggt cttttcccgg taataaagaa gcagccctga
    13861 aagcctggga agagatggcg gttgtggccg gcgataccct ggccaatgcc atcaccctga
    13921 tcgacggaat tatcgtgatc ggcggaggat tatccggcgc ccacggagta ttcctgcaac
    13981 gactcgtgga tgagatgaat gcgagcttta catcgctcga cgggcatgaa gtaccccgcc
    14041 tcgagatcac ggcactcaac ctggaaaacc cggtggaact ggccgagttc ctggaaaaca
    14101 agtcgatcga aatcaaagtg ccgttcagcc aaaaaaccgt caattataac ccggcacggt
    14161 atatcggcgt cggcgtttcc cggctgggca catccagggc cgtatcggtc ggcgcctacg
    14221 cctatgcact ggctgaactg gataagattt agttagaggc tattcgtgtc aattcgcctc
    14281 attcgtgttg attcgtgttc cctttggaat aacggatgag acgaatttta cgaataacca
    14341 cggataaata atagcagaaa tattaaccat tccggatttt atttgttatt aacttcagaa
    14401 agaaatatcc atttatcatg aaactctaca gcatcgaaac cggaaacctg aaactcgacg
    14461 gcggcgccat gttcggcgtc gtacccaaat cactctggtc gaaagtttat cctgccgatg
    14521 agaacaatct gtgcaattgg gccatgcgct gcctgctggt ggtcgatgaa gaccgcagga
    14581 tcctgatcga caacggaatc ggcgacaagc aggactggaa ttttctccgc cattatcatc
    14641 tcaacggaga tgacacgctg gagaagtcgc tggcaaaagc cggtttcggt aaggaggata
    14701 ttacggatgt gatcctgacg cacctgcatt tcgaccattg cggcggaagt gtcgaatgga
    14761 accacgaccg caccgggtac aagctggctt ttcccaatgc caattactgg gtcagccgtc
    14821 agcagtacaa ctgggccgtt gagcccaatc atcgcgaaag cgcctcttat ctgaaagaaa
    14881 acattttacc cattgaagag agcggaaagc tgaaactaat tgaaggcgaa ggtgaaattc
    14941 ttcccaacat caccataaaa ctttaccatg gccatacaga cggacaggcg atacctcaca
    15001 ttcgatacaa cggtaaaact gtggtattta tggccgatct gcttccctca acagcccatg
    15061 tgccgatgcc gtggatcatg ggttacgata cccgcccgtt gattacttta gaagacaaaa
    15121 cccgctttta cagggaagcg ctggaaaacg actacatcct gttcctggaa catgatcttt
    15181 accatgaatg ctgtaccctt caccagaccg acaaaggcgt taaagtaaag gaaacctttg
    15241 aactttgaac taattctagt cttcgtcttt cgcgctttct tcgtattcct tcagcagttt
    15301 ctcctggata tcggacggta ccggggcata ttcgttgaat ttcatcgtgt agcttgcacg
    15361 gccgctggtg agcgagctta aagccgtcga atagcggttc agttcggcaa gcggcacgcg
    15421 ggcacggatc gttccgccat ccatacccat aatgatggca cgccttcctt gcaggtcggt
    15481 catgacgcca cccattttat cggccgggac gaagacttcg acatcataga tcggctcgag
    15541 gatcttcggg ctggcttcct tgaaagccat gctgaaggcg gtacggccgg cgagtttgaa
    15601 ggagatttca ttggaatcga ccgggtgcat cttgccatca taaacataga cgcggatatc
    15661 gcgggcatag gaaccggtaa gcgggccctg ctccattttc tccatcaatc ccttgaggat
    15721 agcgggaagg aaacgggtat cgatcgaacc gccgacgatg cagttataga aaacgagttt
    15781 tccgccccat tcgagctgga tctcgtcttt accgcggatc gacagtgaaa tttcggtatc
    15841 gcctttggaa atagtcagcg gggtgaccga cgactggcgc atcatttgtt tcagcttgac
    15901 gctcgacggg tcgggtttgc cctcttcgtg cggctcgatg accatgtgca cttcgccaaa
    15961 ctggccggcg ccgcccgact gcttcttatg ctggtaggta gcctgggcaa ctcttgtgat
    16021 cgtttccctg taaggaatac gcggaggaag gtagttcacc tgtatcttga tattgttttc
    16081 caggtgccac ttggcaatgt tcaggtgaag ttcgccctgg ccctccagga tcagctggcg
    16141 caattccttc ttgtaaccgg caatgatcgt ctggtccatc ttgtgcaact cattcagcgc
    16201 catgcccagt ttttcgtcat cggcctgatt gacggcttta caagccacgc ggaacttcga
    16261 atcgggatac tcgatcgggg cgatcttctc atccgcaaac ttgggagcat tcagcgtatg
    16321 gttggtcagc gtattcttca gcttgatggt agcgccaata tcaccggcga ccatcttttc
    16381 caccttgtcg cggttcttgc cggcaaagac gaacacctgc gagagacgct ccttggtggc
    16441 attggtgccg ttgatcatat cttctgcttc atggatctca ccgctgaaaa ccttgaaaaa
    16501 gctcacctca cccaggtgct gctcgatggt cgttttgaag acaaaagcca tagccggctc
    16561 ggtagccaga catttgacct cttttcccga gacagtcttg acaggcggca tttcattcgg
    16621 ggcggaaaca ttgttgatga tgaattccag caaccggccc acgcccatat tctgtttggc
    16681 attgacacaa agcaccggga aaatgcccct gttgcgcaaa cccaggcgca gcccgcggga
    16741 gatttcctct tccgacaaag tcccgttttc gaagaacgat tccatcagcg attcatcgtt
    16801 ggctgccaga tcttcgatca gctttgcctg caattcttcc gctttgcctt tttcattggc
    16861 aggaatatcc agaatctccg gttttccccc tccatccggg tatttcagca ttttcatctt
    16921 aagaaggtcg atgaccgaat taaaaccatg tccggtgtga agcggatact ggaccaccgt
    16981 aaccgcgctg ctgaaggagc tcttgagctg gctgacggta tcgtcgaaat tcgagttgtc
    17041 gtgttccagg tggttgatgg caaagatcac cggcatgagt tctttggtac aaagcctcca
    17101 ggttgtctcc gttcccggtt caacgccatt ctgtgcattg accacggata cggctgcatc
    17161 caccactttc atcgcggaaa ttacctctcc cacaaagtca tcaaaacccg gcgtatcgat
    17221 aacattgatc ttgtgattgt tataaatgga atacaaaacg gatgaataga ctgaattctg
    17281 tctttccatt tcaatatcac ggtaatcgga aaccgtattt ttatcttcga tcgatcccct
    17341 cctgttaatt acgccgcctt cgaacaacat atcttctgcc aatgttgttt tacccgtctt
    17401 ggcgccgcct atcaacgcga tgttcctgat ctcgttcgtt tgaaatacct tcattttctg
    17461 taatttataa ttgttaacct gttaattccg aataagggac gcaaaagtag aaaaaagctg
    17521 ccaactttca aagaagccgc tgaaaaaagt cttccataac cttaacttgc tgaaagttcc
    17581 cgataacttt ttaactattt acacatttaa aaaataattt tacttttatt tcaagtctta
    17641 aaacccattt taatgataag gaaagcgcta ccattcctgg taatattgtt tttctccata
    17701 accctggccg gacaagaaat tcccgaggca taccggaatt ataatgcgaa atatccgtcg
    17761 gggccggtta tgaccaaccg ggatgcggct cttcttcgct ccctgccaga aaaaaaggtt
    17821 ccggaaaccg cagtgaggga ccagcttccg gcaattgtcg ataattcaac ccttccttac
    17881 ctgaggccgg tatttctgca ggagggcccc agttgcggac aggcagccat ggtcggttat
    17941 aatttcacat acgaaatggc ttgcaagaga gaccagcccg ccttgttccc gcaaacccag
    18001 tatccgaccc atttcacctg gaattttcag aacggtggcg atggctggta tggagtcagc
    18061 tatttccaca gtgtcgaaat cctccgcatg tgcgggtgta tgaattctta cgactatggc
    18121 ggttttttcg atgacgggcg ccgctggatt aacggttaca acctgtataa caatggtatg
    18181 tacaacaggg tcaggcagat gtaccggatc aacacctcga cggaagaagg aatcctggca
    18241 ctgaagcact ggctctacaa tcatatggga gaacaggaac atggcggcgt tgcgagctac
    18301 tacgccaatg tcccctggaa tgcagttttc ctcaatgata cgactcccga aggaggcaaa
    18361 tacgtagtga cctggtggta cccgctggct tcccacgcca tgaccattgt gggttacaat
    18421 gattcgatcc gctacgatta caacggcgac gggcaataca ccaatactat cgatcttaac
    18481 aacgaccagg tcatcgaccc cagggactgg gagttcggcg gagtaaagtt tgtcaacagc
    18541 cacggaaccg ggtcgcagga cagtggtttt tgttacatga tgtacaaggt tctggccgaa
    18601 aagtttgaaa caggcgggat ctggaaccag gaggttcata tcctggatgt tgatgaagat
    18661 tatttgcccc tgatggcgtt taaagtaaaa ataaaacata ctaaccgggg gaagattaaa
    18721 gtgatggcag gtgtttcaca ggatacatcg gatat
//
LOCUS       DAOPNH010000024        18158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2971_length_18158_cov_6.185107, whole genome shotgun sequence.
ACCESSION   DAOPNH010000024 DAOPNH010000000
VERSION     DAOPNH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_18158_cov_6.185107"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1754
                     /gene="ftcD"
                     /locus_tag="PKZ74_02400"
     CDS             54..1754
                     /gene="ftcD"
                     /locus_tag="PKZ74_02400"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPM91631.1"
                     /translation="MKQLIECVPNFSEGRDMTIIKQITDQIEGVEGVRLLDVDPGKAT
                     NRTVVTMVGEPEAVLEAAFLAVKKASEVIDMRNHKGEHPRFGATDVCPLVPIRNITME
                     ETAALARKLAERIGKELGIPVYCYENAAFTEERRNLANNRSGEYEGLPEKLKNPQWKP
                     DYGPAEFTERVALSGATAVGARDFLVAYNVNLNTTSTRRANAIAFDIREKGRPVREGN
                     PITGKIKKDENGNEVWTPGSLKACKAIGWFIEEYGIAQISINLTNISITPVHVAFEEA
                     CAKANERGLRVTGSELVGLIPLKAMIDAGKYFLRKQQRSVGVSENELVKIAVKSLGLD
                     DLKPFNPKEKIIEYLLEDPSDKKLIGMTVEGFADETASESPAPGGGSISALCGALGVS
                     LGTMVANLSSHKAGWDDRWEEFSVWAEKGQQYKDELLFLVDEDTRAFNRIMDAFGLPK
                     GSDDEKNARKAAIEEASKYAIKVPFRVMEKSLASMEVMKAMAETGMAASASDVGVGAL
                     CARTAVMGAYLNVKINAGGLEDKAFAEEMVKKGEEIVEKARILEDEILAVVEKKIRKI
                     "
     gene            1905..3290
                     /locus_tag="PKZ74_02405"
     CDS             1905..3290
                     /locus_tag="PKZ74_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HPM91632.1"
                     /translation="MLYSETVQNPVRLTIHLRGVHSSKISLMTQKKGSPLIQDILVKD
                     NVNNGDTCVFIVPAEYLPGEFVLRFDYKEEVTSTPYPSEKSFILNRQDMELWVSPVFC
                     NNSDSTRWQDGELENAALHEFMAENYRQKEMLGLLQNYLMNYDDNTSGFYRMGITEYE
                     KRRNAHNEWIAGQVERHRDLFVSSLFFFQYVPEVSWEGDEADRRQSFKDHYFDFVDFN
                     DTLLIRARDFKSWMDQYVNLYGEDATTTAKRDSLFTAAGKRAVEKAKTGHPKIYGWMV
                     DYFYNGYESFNIQPGIEMLAAYLDDPDCLTAKRQAILKRVEGMKTLVEGTLAPDFPVK
                     DNSGNQASFHQFKTNTPYKLVLFWSADCPHCMELVNKLYTFWQKPGSREKLSIFAISL
                     DETETEIAEYEKAIRLLPGWNHHLARGGVNSAEAGSYFILATPVMVLTGADNKIVSMP
                     ESVEKLQQELK"
     gene            complement(3556..4389)
                     /gene="cdaA"
                     /locus_tag="PKZ74_02410"
     CDS             complement(3556..4389)
                     /gene="cdaA"
                     /locus_tag="PKZ74_02410"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPM91633.1"
                     /translation="MLAILLTGLFQIRLLDVLDILLVAFILYELYNLLKGSVSINMFF
                     AIVAMFFIWRVTDALQMEMLREILGAFFSVGIIALFIIFQPELRQFLLTLGKPDFIQK
                     RRRRFLFWHFNEASPYVVDVDKIVHACQKMANNKQGAIIIITRLHELKIIKDTGQYLN
                     AEISVELLENIFFKNSPLHDGAVIISGNKIEAARCILPVSKSQDLTAGLGLRHRAAVG
                     VTEQSDAIAIVVSEQTGKISYAESGELVRNVQPSALRDLLEEKLQAVPKERENAVTET
                     A"
     gene            complement(4477..5433)
                     /gene="folP"
                     /locus_tag="PKZ74_02415"
     CDS             complement(4477..5433)
                     /gene="folP"
                     /locus_tag="PKZ74_02415"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPM91634.1"
                     /translation="MIKKVDKNTSFSETKVFEYNGKTMDFRLPRVMGIVNVTPDSFYD
                     WTVGRFGDLTIGRLDDLAVGRFGDLAVGRFGDLAVGRFGDLMGRVKEMADSGAYIIDI
                     GAVSTRPGAPEVSVEEEKSRLLPVLKEVRKSFPEIIISIDTYRSEIAEIAAGEGADMI
                     NDISGGMFDPLMLSCIIKLKIPYIIMHIQGTPATMQQNPTYADVVMEVKDFLLGQVRR
                     LESAGHHKIIIDPGFGFGKTVEHNYQILSHLDEFVDTGYPVLAGLSRKSMINKVLGTK
                     PAEALNGTTVLNTIALMKGASILRVHDVKEATETVKLFIKAI"
     gene            5473..6027
                     /locus_tag="PKZ74_02420"
     CDS             5473..6027
                     /locus_tag="PKZ74_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HPM91635.1"
                     /translation="MNDTSRQFGLAITACKEVFVNKMKDYGSSWRVLRPESLTDQIYI
                     KANRIRSIEQKGQQKVQEGIRPEFIGIVNYSVMALVQLELGIGGNMDLAPEEAGQLYD
                     KHLQSARALMEDKNHDYDEAWRNMRVSSYTDIILMKLMRIKQIEDREGETLVSEGVDA
                     NYRDIINYALFALIKLDSVGQLDS"
     gene            6169..7278
                     /locus_tag="PKZ74_02425"
     CDS             6169..7278
                     /locus_tag="PKZ74_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPM91636.1"
                     /translation="MKLFANICRLLVAAVFLFSGFVKGVDPLGTAYRIEDYFIAFGTG
                     WAIPLSLFLSIFLCAVEFTLGAALLFNFRLKIMAWPLLLMMAFFTILTLNDAIYNPVP
                     DCGCFGDALKLTNWETFYKNVVLIILVIIIFAYRNKYRSIFVPRIDNWGLVIVFLLFS
                     YFSFHQYRHLPWIDFLGWKVGADLVPDNPGQAKTYLMYRNIATGEEKEYLSPNYPWND
                     TVWQKEWEFVSQRTDDSGVLKGHSLQIFDAEGNDLTEVYVNNPGYQFLVASYDLKSAS
                     VKGFKKIDKLHADMVSDGHSLVVLTGSLETDIREFKKGFQPDLEFFNADDVELKMMVR
                     ANPGIILLNDGVVLGKWHWRDLPDYEELKEEFEGL"
     gene            7278..8357
                     /locus_tag="PKZ74_02430"
     CDS             7278..8357
                     /locus_tag="PKZ74_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91637.1"
                     /translation="MIKFILNRLFYGLLVLLGVITLIFILFNILPGDPARMLLGQRAD
                     ISSVEMIRKDLGLDKPLPVQYAGFLNDISPVSGHNHVDQTSYWYLDKEKYSPFLTVLK
                     IGKTSLVVKAPYLRRSYQSKREVASIIAGAFPKTLLLAAVSMLFAFVAGVSIGILSAI
                     YRDGWFDKIAMVLSVLGMSLPSFFAAIIVAWIFAFLLSDLTGLSMTGSLYSVDDFGRG
                     EFLDLKNLILPALTLGLRPLGILTEMTRNSMLDVMSQDFIRTARAKGLSETRVILRHA
                     LKNALNPVITTASGWFASLMAGAVFVEYVFDWKGIGVVVVDALEKYDFPVIMGTVLFI
                     SVIMILINILTDIAYSRLDPRVELG"
     gene            complement(8507..8863)
                     /locus_tag="PKZ74_02435"
     CDS             complement(8507..8863)
                     /locus_tag="PKZ74_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPM91638.1"
                     /translation="MVKEFPDLERLKHISEATGKILHFCRNKSEDDFIKDEMLNSSVL
                     YQFIIIGETIRYINPELLAKYDYPWHLPKSFRNYIANEYFGINLKQVYKTVTDLLPEF
                     KELIDKMIDTITMLNF"
     gene            complement(8856..9446)
                     /locus_tag="PKZ74_02440"
     CDS             complement(8856..9446)
                     /locus_tag="PKZ74_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM91639.1"
                     /translation="MKSFGETIRDLRKKNKLPLRSVADSLGIDVAILSKIERGQRKAS
                     RQFICKISSFYQVDINHLLITWLSDKLAEELIEEDTAMEALQVAEDKIIYGRRKVVDK
                     RQIIDLLVDFFRHDGRISKAWIFGSFARGDNSSHRDIDLMVRYSDKASGTLFDYSDIK
                     FKLENLLKQEIDIVEEGYVQPFALESVNRDKILIYG"
     gene            9745..10515
                     /gene="tpiA"
                     /locus_tag="PKZ74_02445"
     CDS             9745..10515
                     /gene="tpiA"
                     /locus_tag="PKZ74_02445"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPM91640.1"
                     /translation="MRTKIVAGNWKMNKTFEEADTLIFEITDLLEEKGKPDDVDVILC
                     PPFIYLELAGDITEDSPVFVGAQNVSRYENGAYTGEISASMLRSMHIDFAIIGHSERR
                     KYFSESDDELLGKVKAALKEDIAPIFCCGELLEERETRQHFDIVRSQLENTVFQLEPD
                     DFARVIIAYEPVWAIGTGINATSDQAQEMHFFIRDLIWKKYGAEIANDKTILYGGSCN
                     SKNAKELFSMPDVDGGLIGGASLKADEFVAIIMAAGEK"
     gene            10579..11427
                     /gene="prmA"
                     /locus_tag="PKZ74_02450"
     CDS             10579..11427
                     /gene="prmA"
                     /locus_tag="PKZ74_02450"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPM91641.1"
                     /translation="MNFELKVMGFLEIRFAPVPSDEEKSILIAELLPFGFDSFNEEED
                     AVLAYIDSVVFQDVPFKEIEFLQNHPEIEYRIEPLGDINWNKEWESNYQPVLIGGKCF
                     VRAPFHPENPDAQYNILIEPGMAFGTAHHETTRLMALCLMQLDVKGKKVLDMGCGTGI
                     LAVLANKMGAISVTGIDNDEWAWRNAIVNFGHNGISAENAVCGDASDIKEVAYDMILA
                     NINRNVLLQDMEMYFKGLLPGGRLMLSGFYRKDKETILEKAMATGLQFVGDDHENEWT
                     VILLKK"
     gene            11464..15885
                     /locus_tag="PKZ74_02455"
     CDS             11464..15885
                     /locus_tag="PKZ74_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91642.1"
                     /translation="MLPAFLLVMISSGQSVKSLPEDPARFPVELESVFSSVTSPSAGI
                     QIRDMLIPFLEYWNAGLFSEKEQKNIIRQSNIVMGKRLVVFPNLYRYISAVYHLKRFG
                     NEGALTIWMEDFDKQIPESSQGRSEKYLEQYEALFARNRLYESGTYNWYTSDTSIRME
                     YDTAMKIIYRDVLLTCATRKDTSRIYNTSGIFYPGTLEWKGMKGRVTWERVGLDPDSV
                     YADFQDYRINMKKFEYRVDSAGFMNKKYFTRPILGVFKDETLTSPPGPNSTHPQFEAY
                     LNTLEIKDLFRNIDYLGGFTQQGLRITGSGAGDGNASLSLKRGPKEIARIRAKAFRLH
                     QNQVTANPASITIPLDEDSIYHAGLQMKYFVDSAQLLLYQEKDATNRSPFFNSYHDLD
                     MDCGSMEWKIDSNFIDFSTFQVMNDTNLNSFYSGNYYSPAEFYKQQGLDDTNPLYVVK
                     DFCETYGTREIKPDILAHFMKKPVEQVKAMLFNFSSQGFVAYDFVNDKAIVKDKLYHY
                     LDAKAGKIDYDVIGIHSKAVNESNARLNLETKDLDIRGVPMVQLSQAHRVYIFPDSNE
                     IKVKKGLDFVFSGRVQAGPFLFFAHESSFEYDTFKLNMPFIDLIKFRVDDFGKEGGQV
                     TVQSPIADASGKLYIDAPRNKSGKTTIPDFPLFKGEKESYVYYDQDRHYDKEKFAYNV
                     KPFNLAGLDKLQTDDIKFDGYLNTGGIFPDIDQALVVQEDYSLGFKHITPREGYEAYG
                     GKAVFYDTIDLSNKGLRGHGRLEYLTSVTHSEDFLFYPDSMVTSIAEKFNIESQVARV
                     EYPDVAAEEIRQVWFPAKDTMRLLTLKKPFRMFGEKSEMYGDLYYSPDGLTGSGKVNF
                     ESVELASSNYRFQHHTIDADTLDFKLFTKGTQDLAVAARKYRTHVDFEQREVEFRTNQ
                     KGSYVSFPYNNFACYMDNIDWAMDKNEMRLYNDLGTRFANIDNLSRQELLRLDMSGSD
                     LVATNPQADSLSFFSVTARYDLQTYEIDAENVKLIRVADAAIFPDSSFVKISRGGKIN
                     TLKNAAIIADTARQYHTIERADIDITSRFRMSGKGFYQYKSGAEAVQEVPLISISVDS
                     ARHTVATGSIPESLKFMLNPHFAFRGKMDMNSSRKELEFDGGFKTQDECYPGMLKYWV
                     AFDAWINPDSVRIPVQPPVRDMEGRRVDLALQMSDSEDDIYSSWFTLKKYPKDSAIVA
                     PAGEIYFDEWRGSYRIDQSKDKKLPGFEFNPRSCSFITAGEHNLGLKLDYVSLVSYGD
                     VNYMTVPDSTIANLTLSVNFMFYDGSLTIMADSILKSNLKGLDVTRKSYLDFLDFTLG
                     SESGDYKKDMAGIGRNKRLPDVLNRTMILTDVNLYWNSFTNSYISRGPIGVMSLGRDP
                     VNRYMNGFVELMRRRSGDGLFVYLEIDENRYYFFEYRNGILQALSHDKEFVNRINEVK
                     PEKRSMVLPGADQKYEYMVGDKRRMIDFIRKMQSLSY"
     gene            15895..16545
                     /gene="plsY"
                     /locus_tag="PKZ74_02460"
     CDS             15895..16545
                     /gene="plsY"
                     /locus_tag="PKZ74_02460"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPM91643.1"
                     /translation="MTLQIILIVILAYLIGSVPTAVWVGKLFFRLDVREHGSGNAGAT
                     NTIRVLGWKAGLPVFLFDVFKGWFAVKLADFLLTGMMTDETLVFLKIGLAAAVVLGHV
                     FPAFAGFRGGKGVATLLGVGFALFPAAVWIVLAIFIIILLTTGFVSLGSIIGSLVFPF
                     LEIFLFGQENPWLIGLSILVAIFIPITHRNNIKRLIKGKESKFRPTRYGSNASKKM"
     gene            16645..17766
                     /gene="dnaN"
                     /locus_tag="PKZ74_02465"
     CDS             16645..17766
                     /gene="dnaN"
                     /locus_tag="PKZ74_02465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPM91644.1"
                     /translation="MKFIVSSSVLLKNLSALSGVISSSNTLPILDDFLFELKEDQLFV
                     TASDLETTMTVTIPLTKAEEPGSVTIPAKLLLDTLKTFSDIPVIFSINPETLLVELSA
                     GEGKYKLSGHSSDEFPQSPVLEATSVLTVHSGVLVDAINKTLFATGSDELRPVMSGVY
                     CELSPEHLTFVATDAHKLVRYRRMDAHSDQVSSFILPKKPLNQIKGILAGKDLEVNIE
                     YSQTNSMFTFDSFKIICRLIEGKYPNYEAVIPTNNPNVLTVDRGLLLNAIRRVALFAN
                     QSTHQIRFKISGKELVLSAEDIDFSNEAKERLTCSYEGEDMEIGFNSRFLQDMVTNVS
                     SEQVKFEMSAPNRAGLIMPVENENLEEEILMLVMPVMLN"
     gene            17835..>18158
                     /locus_tag="PKZ74_02470"
     CDS             17835..>18158
                     /locus_tag="PKZ74_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM91645.1"
                     /translation="MTQDKYIPEMYRMVDAMDTPGFVNLFTADGIFRWANMEPVAGKE
                     NITAFLTGFFQSIKAISHTDLEYWQVPGVRFATGNVNYTRRDNSLLRVPFSVILKFKG
                     DLINEF"
BASE COUNT         4755 a         4344 c         4511 g         4548 t
ORIGIN      
        1 gaacaataga acaatagaac aatagaacaa tagaacaata gaacaataga acaatgaagc
       61 aactaataga atgtgtcccc aatttcagcg aagggcggga tatgacgatc attaaacaga
      121 tcacggatca gattgaaggt gtggaaggcg ttcggctgct ggatgttgat ccggggaagg
      181 cgacgaaccg gacggtggtg acaatggtcg gggagccgga agcggtgctg gaagctgctt
      241 tcttagcggt aaagaaagcc tcggaagtga ttgatatgcg caaccacaaa ggggaacatc
      301 cgcgattcgg cgccaccgat gtctgtccgc tggtcccgat ccgtaatatt acaatggaag
      361 aaactgcggc tttggccagg aaactggcgg aaagaattgg caaagagctg ggaatcccgg
      421 tttattgcta tgaaaatgcc gcattcacgg aagagcgccg taacctggca aataacaggt
      481 caggggaata tgaagggctg cccgagaaac tgaagaaccc gcaatggaaa cccgattacg
      541 gtccggctga gtttactgaa agggttgccc tgtcaggcgc aacggccgtc ggcgccaggg
      601 atttcctggt ggcgtacaat gttaacctga acacgacttc cacccgccgg gccaacgcca
      661 ttgctttcga tatccgcgag aaaggacgac cggtgagaga aggtaacccc atcaccggaa
      721 aaataaaaaa agacgaaaac ggcaacgagg tttggacccc cggctctttg aaagcatgca
      781 aagcaatagg ctggtttatt gaagaatacg gcattgctca gatctccatc aacctgacaa
      841 atatcagcat tacgccggta catgtggctt ttgaggaagc ctgtgcaaaa gcgaatgaac
      901 gcgggttacg ggttaccggg agcgagctgg tgggattaat tccgctgaaa gccatgattg
      961 atgccgggaa atatttcctc cggaaacagc aacggtctgt cggtgtctcg gaaaatgaac
     1021 tcgtgaagat cgcagttaag tcgctgggcc tggatgacct gaagcccttt aatccgaagg
     1081 agaagatcat tgagtacctg ctggaggatc cttccgacaa gaaactgatc ggtatgaccg
     1141 ttgaaggctt tgccgacgaa accgcttcgg aatcgccggc gcccggcgga ggctccatct
     1201 cagccctttg cggtgcactg ggcgtttcgc tcggaaccat ggtggccaat ctctcctcgc
     1261 acaaagcggg ctgggacgac cgctgggaag agttctccgt ttgggccgaa aaaggccagc
     1321 agtacaagga cgaactgttg ttcctggtcg atgaagatac ccgtgctttc aacaggatca
     1381 tggatgcgtt tggtctgccc aaagggtccg acgacgagaa aaatgcccgc aaagccgcta
     1441 tagaagaggc ttcgaaatat gcaataaagg ttcccttccg ggtgatggaa aaatcgctcg
     1501 cttcaatgga agtgatgaaa gccatggcgg agactggaat ggcggcgtcc gcctcggatg
     1561 taggcgtagg cgcgttgtgc gcaaggaccg ccgtcatggg cgcctacctg aatgtaaaga
     1621 tcaatgccgg cgggctggaa gacaaggctt tcgccgaaga gatggtgaag aaaggggagg
     1681 agatcgtgga gaaggcaagg atcctggaag atgagatact ggcggtggtg gagaagaaga
     1741 tcaggaagat ttgaggattt gggattttga gttttctttt tatacattta cacggtaatc
     1801 caggtcttta gtgttcactt aatatgcttc atattatggg aattgtctgc tttaacagag
     1861 ttgcaaaagt gtcaatgctt gttgcctgct taatcacaag ccggatgctt tattcggaga
     1921 cggtccaaaa cccggtcagg ctcaccatcc acctccgtgg cgtgcattcc agcaagatca
     1981 gcctgatgac ccagaaaaaa ggcagcccgc tgatccagga tatcctcgtt aaagacaacg
     2041 tgaacaatgg tgatacctgt gtctttatcg tgccggccga atatctcccg ggcgaattcg
     2101 tcctacgctt cgattataaa gaggaggtga cctcaacacc ttatccctcc gagaaaagtt
     2161 tcatccttaa ccggcaggac atggaattgt gggtatcacc ggttttctgc aacaattccg
     2221 acagcacacg gtggcaggat ggcgaactgg agaatgcagc cctgcatgag tttatggccg
     2281 agaattaccg gcagaaggag atgcttggcc tgttgcagaa ttacctgatg aattatgatg
     2341 ataacacctc cggtttttac cggatgggga ttaccgaata tgaaaagcga cggaatgcgc
     2401 ataatgaatg gatagccggc caggtagaac gccaccgtga cctgttcgtg tcgtcgctgt
     2461 ttttcttcca gtatgtcccc gaagtgagct gggaagggga tgaggccgac cgaaggcaga
     2521 gtttcaaaga tcattatttt gattttgtcg atttcaacga taccctgctg atccgggccc
     2581 gtgattttaa atcatggatg gaccagtatg tgaacctgta cggggaagat gcgaccacaa
     2641 ccgccaaacg cgactcgctg ttcacggctg ccggtaaaag agcggtagag aaagcgaaaa
     2701 caggccatcc gaaaatatac ggatggatgg tcgattattt ctacaacggt tatgagagct
     2761 tcaacatcca gcccggtata gagatgctgg ctgcctacct cgatgatccc gactgcctga
     2821 cagccaagcg ccaggcaatc ctgaaaaggg tggaagggat gaagacactg gtcgaaggga
     2881 cgctggcgcc cgatttcccg gtgaaagaca attcgggaaa ccaggcctct tttcatcaat
     2941 ttaaaacaaa taccccttat aagctggtcc ttttctggtc ggccgactgc ccgcactgca
     3001 tggaactggt caacaagctg tacacattct ggcaaaaacc gggttccagg gaaaagctgt
     3061 ccatttttgc catcagcctt gatgaaaccg agaccgaaat tgctgaatac gaaaaagcga
     3121 tcaggttgct tccgggatgg aaccatcacc tggcccgggg cggggttaac agcgcggaag
     3181 ccggcagcta tttcattctg gccactccag tcatggtgct caccggtgca gataacaaga
     3241 tcgtttcgat gcccgaatcg gtagaaaaac ttcagcagga gttgaaatag aatggtttaa
     3301 ttaatacaaa gtttttttct tgccccaggg gcgttttatt taagaggcgg gctcataagt
     3361 ccaaatattt ggctttttta ggtggtccga cgactgaagc cgtcggttca aaccttaata
     3421 aaacattagg aaccaacggc tttagtcgtt ggagcacgat ctattttcag gctatcaatt
     3481 tgaaatatca gactttggag acagtttcta ttgtgatacc cgctgcttga ggggtgaaat
     3541 tcattggcaa gtgtatcagg ccgtttcggt caccgcattt tcccgctctt tgggaaccgc
     3601 ttgcagtttc tcttccagca gatcgcgcag ggccgacggc tggacattcc gcaccagttc
     3661 accgctttcg gcataggaaa tctttccggt ttgttccgac accacaatcg caatggcatc
     3721 gctctgttcc gttacgccaa cggctgcccg gtggcgcagg cccagcccgg cggtcagatc
     3781 ctggctcttg gagacgggca aaatgcaacg ggccgcttca atcttattgc cgctgatgat
     3841 cacggcgccg tcatgcagcg ggctgttctt gaaaaagatg ttttccagca attcgacgga
     3901 gatctctgca ttcaggtact ggccggtatc cttgatgatc ttcagttcgt gcagccgggt
     3961 gatgatgatg atggccccct gtttattatt ggccattttc tggcaggcat ggacgatctt
     4021 atcaacatcg acaacataag ggctggcttc gttgaaatgc cagaaaagga accggcgacg
     4081 ccgtttctgg atgaaatcgg gcttgcctaa tgtcaggagg aactgcctga gttcgggttg
     4141 gaaaatgata aacaaggcaa tgatacccac gctgaagaaa gcccccagga tttcgcggag
     4201 catctccatc tgcagcgcgt cggtgacccg ccagataaag aacatcgcca cgatggcaaa
     4261 gaacatgttg atagagaccg aacctttcag gaggttataa agttcataca ggataaacgc
     4321 aaccagcagt atgtcaagta catccagcag gcgaatctgg aacaagccgg taagaaggat
     4381 tgcaagcatg ggatacaaaa gtagggaaat taggtaattt gaaaattatt caatgtgaga
     4441 atttgataat aacttcatta tcacatcatc acattatcaa attgctttta tgaacagttt
     4501 caccgtctcc gtcgcctcct tcacatcgtg caccctcagg attgacgctc ctttcatcaa
     4561 tgcaatagta ttaagcaccg tagtgccgtt taaagcttcc gcaggtttag tccccaagac
     4621 tttattgatc atggatttgc gcgacaggcc ggcaaggacg gggtaacccg tatcaacgaa
     4681 ttcatccagg tgcgacagga tttgataatt gtgctctacg gtctttccaa agccgaaccc
     4741 gggatcgata atgattttat gatggccggc cgactccagt cttcggacct ggccaagcag
     4801 gaaatccttt acctccatca ccacatcggc ataggtcgga ttctgctgca tggtggcggg
     4861 cgtcccctgg atgtgcatga tgatgtaagg aatttttagt ttaatgatac acgaaagcat
     4921 aagggggtcg aacattcctc cggagatatc gttaatcata tcagcgcctt caccggccgc
     4981 tatttcagca atttccgacc ggtacgtgtc aattgaaata ataatctccg gaaatgattt
     5041 tcttacttcc ttaagaaccg gcaacaatct gctcttctcc tcttcaacgc ttacttcagg
     5101 cgctcccggc cgcgtcgaaa ccgcccctat atcgataata tacgctccac tatcagccat
     5161 ttccttcaca cgccccatta aatcgccaaa tcgtccaacc gccaaatcgc caaaccgccc
     5221 aaccgccaaa tcgccaaatc gtccaaccgc caaatcgtcc aatcgcccaa tcgtcaaatc
     5281 gccaaatcgc ccaaccgtcc aatcataaaa gctgtcaggc gtcacattca cgattcccat
     5341 cacccgcggc aagcgaaagt ccatcgtctt gccgttgtat tcaaaaactt tggtttcgga
     5401 aaaagatgta tttttatcaa cctttttgat catatttaac cggattaatt aattgcgaag
     5461 ataatagaaa tgatgaacga tacctcccgg caattcggac tggcgatcac tgcctgtaaa
     5521 gaagtgtttg tcaataagat gaaagactac gggagttcgt ggcgggttct gaggcccgaa
     5581 tcgctgaccg accagatcta tatcaaggcc aaccgcatcc ggtcgatcga gcagaaaggg
     5641 cagcaaaaag tgcaggaagg gatccggccc gagttcatcg ggatcgtgaa ctactcggtg
     5701 atggcgcttg ttcagcttga actgggcatc gggggcaata tggacctggc cccggaagag
     5761 gccgggcagc tttacgacaa gcatctgcaa agcgccaggg cactgatgga agacaaaaac
     5821 cacgattacg acgaggcctg gcgcaacatg cgggtgagct cctataccga tattatcctc
     5881 atgaaactga tgcggatcaa acagatcgaa gacagggaag gggagacgct ggtgtcggaa
     5941 ggggtggatg cgaattacag ggatattatc aattacgcat tatttgcttt aataaaactg
     6001 gattcagttg gacagttgga cagttgacag cttgtcccga gcgaagcgag ggattggaca
     6061 attggacagt tggacaatag aaattagaaa ttagtaattt gaacttggaa ccaggaactt
     6121 ggaactttgg aaccaggaac tttggaaccc tggaactaat tgactcatat gaaactcttt
     6181 gctaacatct gccgtctgct cgtagccgct gtattcctct tttcaggatt tgtcaaaggc
     6241 gttgatccat tgggtaccgc ttaccggatc gaagactact ttatcgcctt cggcaccgga
     6301 tgggccatcc cgttgtcgct cttcctgtcg atctttctct gtgccgttga gttcacgctg
     6361 ggcgccgccc tcctgttcaa tttccggctg aagatcatgg cctggccgct gctgctgatg
     6421 atggctttct ttacgatcct cacgcttaac gatgccatct acaatcccgt tcccgattgc
     6481 ggctgctttg gcgatgcgct gaaactgacc aactgggaaa ccttctacaa aaatgtcgtc
     6541 cttatcatcc tggtcatcat catatttgct taccggaata aataccgttc catatttgtc
     6601 ccccggatcg acaactgggg tttggtcatc gtattcctgt tattcagcta tttctccttt
     6661 caccagtacc gccacctccc gtggatcgat ttcctgggct ggaaggttgg cgccgacctg
     6721 gtccccgaca atcccggcca ggcaaagact taccttatgt accgcaacat tgctaccggg
     6781 gaagagaaag agtacctctc cccgaattac ccctggaacg acacagtatg gcagaaggaa
     6841 tgggaattcg tgtcgcagcg gaccgatgac tccggcgtcc tgaagggcca ttcgcttcag
     6901 atcttcgatg cggaaggcaa cgacctcacg gaagtctatg tgaacaatcc cgggtaccag
     6961 ttcctggtag cctcgtacga cctgaaatcg gcttctgtca agggctttaa aaagatcgac
     7021 aagcttcatg cggacatggt ttcggatggt cattcgctgg tggtgctcac cggcagcctc
     7081 gagaccgata tccgggagtt caaaaagggt ttccaacccg acctggaatt cttcaacgcc
     7141 gatgatgtgg agttgaagat gatggtgcgg gccaacccgg ggatcatttt gctgaacgac
     7201 ggcgtggtat tgggtaaatg gcactggcgg gatctgccgg attatgaaga attgaaagag
     7261 gagtttgaag gattgtagtg ataaaattca tcctcaaccg gttattctac ggactgctcg
     7321 tcctcctggg cgtcatcacc ctcattttca tcctcttcaa catcctcccc ggcgatccgg
     7381 cacggatgct gctgggccag cgggccgata tctcttccgt cgaaatgatc cgcaaggacc
     7441 tcgggctcga caaaccgctg cctgttcagt acgccggctt tttgaatgac atctccccgg
     7501 tttcagggca caaccatgtg gaccaaacca gctattggta cctggataaa gaaaaatact
     7561 ctccctttct tacggtgctg aagatcggga aaacgagcct ggtcgtcaaa gcgccttacc
     7621 tgcgccgctc ttaccagtcg aaacgggaag tcgcttcgat catcgccggg gcctttccca
     7681 aaacgctgct gctggccgcc gtttcaatgc tgtttgcctt tgttgccggc gtttccatcg
     7741 gcatcctgag cgctatctac cgcgacggct ggttcgacaa aatagcgatg gtgctgtcgg
     7801 tcctgggcat gtcgcttcca tcctttttcg cggcgatcat cgttgcctgg attttcgctt
     7861 tcctgctgtc ggacctcacc ggcctgagca tgaccggcag cctttattct gtggacgatt
     7921 tcggcagggg agaattcctg gacctgaaaa atcttatcct tccggccctg acactgggat
     7981 tgcggccgtt gggaatcctg accgagatga cccggaactc gatgctcgac gtgatgtcgc
     8041 aggattttat ccggacggcg cgggccaaag ggctcagcga aacgcgggtc atcctgcgcc
     8101 acgctttgaa gaacgctctg aacccggtca tcaccacggc gtcgggctgg tttgcctcgc
     8161 tcatggccgg cgcggtgttc gtcgaatatg tcttcgactg gaaagggatc ggcgtggtcg
     8221 tcgtcgatgc cctcgaaaaa tacgatttcc ccgtaataat gggtacggtg ttgttcatct
     8281 cggtcatcat gatcctcatc aacatcctga ccgatatcgc ctattcccgc cttgatccga
     8341 gggtggagct ggggtaggct taagttcaaa gttcaaagtt caaagttcaa agttcaaagt
     8401 tcaaagttgt gggtgggcag taggcagtag gcagtgtgta gtgggcagcc tgtactgagg
     8461 gcggagccca aaggatcagc agtcattaag aattaagaat taaaaattaa aaattaagca
     8521 ttgttattgt atcaatcatc ttatcaatca attctttaaa ctccggtaat aagtcggtga
     8581 ctgttttata aacttgtttc aggttaatac cgaaatattc atttgcaata tagttcctga
     8641 atgattttgg cagatgccag gggtaatcgt attttgccag caattcagga tttatatatc
     8701 ggatggtttc gccaatgata atgaattggt ataagacact actgttcagc atttcatcct
     8761 taataaagtc atcttcagat ttatttctac aaaaatgaag gatctttcct gtggcttctg
     8821 agatatgctt taacctttcc aggtccggga attccttaac catagatcag tattttatcg
     8881 cggttgaccg attcaagagc aaatggctga acataacctt cttcaacaat gtcaatttct
     8941 tgtttcagta gattctccag cttgaatttg atatcggaat aatcgaaaag tgttcctgag
     9001 gctttatccg aataccttac catcagatca atatcccggt gggaagaatt atcgcccctt
     9061 gcaaacgagc caaaaatcca tgctttagaa attcttccgt cgtgcctgaa gaaatcgacc
     9121 agtagatcaa tgatttgtcg cttgtccacc acctttctcc tgccgtaaat gatcttgtct
     9181 tcagcaacct gtaaggcttc catcgcggta tcttcttcta tcagttcttc cgcgagttta
     9241 tctgagagcc aggtgatcag caaatggtta atatccacct ggtaaaatga ggaaatttta
     9301 caaataaact ggcgtgatgc ttttctttgt cctctctcga ttttactgag aatggccacg
     9361 tcaataccca atgaatccgc aacagacctt agcggaagct tattcttttt ccttaaatcc
     9421 cggatagttt ctccgaaact tttcatggca aatcattttg acaaaattag tcaaaaaaca
     9481 ttgaacactg agaaatgaga aaagagacaa ggggctaatt ttctgccgta aaactttaaa
     9541 cacgaatgag actccatctc gatggcccag atggagtctc gattcttgtg agactccatc
     9601 tcatcggttt gagatggagt ctcgaattgt gcctatgtgg ccaataaaaa tcccccagct
     9661 taagactaaa ttgatttttt tgacttcctg ctgcctactg ccaactgcct acttttttaa
     9721 tatctttgcc aaaaacacca ccgtatgaga accaagattg tagccggcaa ctggaagatg
     9781 aacaaaacct tcgaggaagc cgataccctt atttttgaaa tcaccgacct gctggaagaa
     9841 aaaggcaaac ccgatgatgt ggatgtgatc ctctgccccc cgtttattta cctggaactg
     9901 gccggggata tcaccgaaga ttcacccgtg tttgtcgggg cacagaatgt gagccggtac
     9961 gaaaacggag cctataccgg cgaaatctcc gcctccatgc tccgttcgat gcatatcgat
    10021 ttcgccatta tcggacactc ggaacggcga aagtatttca gcgagtcgga cgatgaactg
    10081 ctggggaagg tcaaagccgc cctgaaagag gatatagcac cgattttctg ctgcggcgaa
    10141 ctgctggaag agcgtgaaac gcgccagcac ttcgatatcg tccgctcgca gctcgaaaat
    10201 accgtcttcc agcttgagcc cgacgacttt gcccgggtga tcatcgcgta tgaaccggtc
    10261 tgggctatcg gcacaggcat caacgccacc tccgaccagg cccaggagat gcacttcttt
    10321 atccgcgacc tgatctggaa aaagtatggc gctgaaatag ctaacgataa aaccatcctt
    10381 tacgggggaa gctgcaattc caaaaacgca aaggaactgt tctccatgcc cgacgtcgac
    10441 ggaggcctga tcggtggcgc ttcgctcaaa gcggatgagt ttgtcgcgat cattatggca
    10501 gccggagaaa aatagttcaa agttcaaaga tcaaagtgca aagtttttta tgtttgcttt
    10561 gactttgaac tttgaacttt gaactttgaa ctaaaagtga tgggttttct cgaaatcaga
    10621 tttgcaccgg ttccgtcgga cgaagaaaaa tcgatcctga tcgccgagct gttgcctttt
    10681 ggtttcgata gttttaatga agaagaagat gccgttctgg cttatatcga ctctgttgta
    10741 tttcaggatg ttcctttcaa agagatcgaa tttctgcaaa atcatccgga aatagaatac
    10801 cggatcgaac cactcggaga tatcaactgg aacaaagaat gggagtcgaa ttaccagccg
    10861 gtgctgatcg gcggtaaatg ctttgtcagg gcgccttttc atcctgaaaa tccggatgcg
    10921 caatacaata tcctgatcga accgggaatg gccttcggta cagcccacca cgagacgacc
    10981 cgcctgatgg cgctttgcct gatgcaactc gatgtaaagg ggaaaaaagt actggacatg
    11041 ggttgcggca caggcatcct ggccgtcctg gccaataaaa tgggcgccat aagcgttaca
    11101 ggtattgata acgacgaatg ggcctggcgt aatgcgatcg tcaatttcgg acataacgga
    11161 atttcagcgg agaatgctgt atgcggcgat gcatcagata tcaaagaagt ggcttatgat
    11221 atgatcctgg ctaatatcaa ccgcaatgta ctgctccagg atatggagat gtatttcaaa
    11281 gggctgctgc ccggcggcag gctgatgctc agcggttttt acaggaaaga taaggaaact
    11341 atcctggaaa aagccatggc gacaggattg caattcgttg gcgacgatca tgaaaatgag
    11401 tggactgtca ttcttctgaa aaaataatac cggctgctta tgtcattcag gaaaatttct
    11461 ttgatgctcc cggctttcct tctggtgatg atctccagcg gccagtcagt aaaatcattg
    11521 ccggaagatc cggcccggtt tcccgtggaa ctggaatcag tcttttcatc agtcaccagc
    11581 ccgtcggcag gaatacagat cagggatatg ctcatacctt tcctggaata ctggaacgcc
    11641 gggctcttca gtgaaaaaga gcaaaaaaac attatccggc aatccaatat tgtcatgggc
    11701 aaacgactgg tcgtttttcc aaacctgtac cgttatatat cagcggttta tcacctgaaa
    11761 agatttggta atgaaggcgc gctgaccatc tggatggaag acttcgacaa acaaatcccc
    11821 gaatcttccc agggccgatc ggaaaaatac cttgagcaat atgaagcgct cttcgcccgg
    11881 aaccggctct atgaatcggg tacttataac tggtacacat ccgatacctc catccggatg
    11941 gagtatgata cggccatgaa gatcatttac cgcgatgtgt tgctgacctg cgccacccga
    12001 aaagacacct cgcggattta caatacttcg ggtatctttt atcccggcac cctggaatgg
    12061 aagggcatga aaggcagggt aacctgggaa agagttggat tggatcccga ttcggtctat
    12121 gccgattttc aggattaccg gataaatatg aaaaagttcg aataccgggt cgattcggcc
    12181 gggttcatga ataaaaagta ttttaccaga ccgattctcg gcgttttcaa agatgaaacc
    12241 ctgacgagcc cgccgggtcc caattcgact catcctcaat tcgaagccta cctgaatacc
    12301 ctggagatca aagatttgtt tcgaaatatc gattacctgg gcggatttac ccagcaaggg
    12361 ctcaggatca ccggttccgg cgccggagat ggaaatgcca gcctgtcgct gaaaagagga
    12421 ccaaaggaaa tagcccggat acgagccaaa gccttccggc ttcaccaaaa ccaggtcacg
    12481 gctaatcctg catccatcac cattcccctg gatgaggatt cgatctacca cgccggattg
    12541 cagatgaaat attttgtcga tagcgctcaa ctgctccttt accaggaaaa agatgccacg
    12601 aaccgcagcc cgtttttcaa tagttaccat gacctggata tggattgcgg atcgatggaa
    12661 tggaagattg acagcaattt tatcgatttt tccaccttcc aggtgatgaa tgataccaat
    12721 ctgaattctt tctattccgg caattattat tctccggcag aattttacaa acaacagggg
    12781 ctcgatgata ccaacccgct ttacgtggtt aaagattttt gtgagactta tggaacccgt
    12841 gagattaagc ccgatatcct ggctcatttt atgaaaaaac ccgtggagca ggtcaaggcc
    12901 atgttgttca atttcagttc acagggattt gtggcttatg attttgtgaa cgataaagct
    12961 attgtcaaag ataagttgta tcactacctg gatgccaagg ccggaaaaat tgattatgat
    13021 gtcatcggta tccattccaa ggcggttaac gagagcaatg ccaggctgaa cctggaaacc
    13081 aaggacctgg atatccgcgg ggttccgatg gtccagctta gccaggcaca cagggtttat
    13141 atcttccccg acagcaacga aataaaggtg aagaaaggcc tcgattttgt gttctcaggc
    13201 agggtgcagg ccggaccttt cctttttttc gcgcatgaat cctcctttga atacgatact
    13261 tttaagctga atatgccttt tatcgacctg ataaaattca gggtggacga ttttggtaag
    13321 gaaggcggcc aggttacagt ccagagcccg attgccgatg ccagtggaaa actatacatc
    13381 gacgcaccca ggaataaatc gggaaaaact accatacccg attttcctct ttttaaaggt
    13441 gaaaaggaat cttatgttta ctacgaccag gatcgtcatt atgataagga aaagttcgca
    13501 tataatgtca aaccgttcaa tctcgccggg ctcgataagc ttcaaaccga tgatataaaa
    13561 tttgacggtt acctgaatac aggaggcatt tttccggaca ttgatcaggc cttggtcgtc
    13621 caggaagatt attcactggg ttttaaacac attactccca gggaaggtta tgaagcttac
    13681 gggggtaaag cagtcttcta tgacactata gatttgagta acaagggact gcgaggacat
    13741 gggcgacttg aatacttaac gtctgtcacc cattcggagg attttctctt ttacccggat
    13801 tctatggtaa cctcgatcgc cgaaaaattc aatatcgaat cccaggttgc cagggtggaa
    13861 tacccggatg tagccgctga agaaatccgc caggtctggt tccctgcaaa ggataccatg
    13921 cgtttgctga cgctgaaaaa gcctttccgg atgttcggcg aaaaatcgga aatgtatggc
    13981 gacctgtatt attcgccgga tgggctgacc ggctccggca aggttaattt cgagagcgtg
    14041 gagctcgcat ccagcaatta ccggttccag catcatacca tcgatgccga tacgcttgat
    14101 tttaaactct ttaccaaagg cactcaggat ctggccgtgg cagcccggaa ataccgtacg
    14161 catgtcgact tcgagcagcg cgaagtggaa ttccggacca atcagaaggg ttcttatgta
    14221 tcctttcctt acaacaactt tgcctgttac atggataata tcgactgggc catggataaa
    14281 aatgagatgc ggctttacaa cgacctggga acccgatttg ccaacatcga caatttaagc
    14341 cggcaggaat tgctccggct cgatatgtcg ggttccgatc tcgtggccac taaccctcag
    14401 gccgactccc tctctttttt ctcggttaca gcccggtatg acctgcaaac ctacgagatt
    14461 gatgcggaaa atgtcaaact gatccgggtg gctgatgcgg ccattttccc agacagttcc
    14521 tttgtgaaga tcagcagggg aggaaagatc aatacgctga aaaatgcggc aatcattgcc
    14581 gatacagccc ggcagtatca tacgattgaa agggccgaca tcgatatcac ctcccggttc
    14641 agaatgtccg gcaaaggatt ttaccagtat aagagcggcg ctgaagccgt tcaggaggtg
    14701 cctttaattt cgatctcggt cgattcggcc aggcataccg tggctacggg ctcaatcccc
    14761 gaaagcctga aattcatgtt aaatccacat tttgctttca ggggcaaaat ggacatgaat
    14821 tcgtcgcgaa aggaactgga gttcgatgga ggatttaaaa cccaggatga gtgttatccc
    14881 gggatgctca aatactgggt tgcctttgat gcctggatca atcccgacag cgtgaggata
    14941 ccggtccagc cgcctgtcag ggatatggaa ggacgtcgcg tcgatctggc tttgcagatg
    15001 tcggattcgg aagatgatat ttactcttcc tggttcacgc tgaaaaagta tcccaaagat
    15061 tcagctatcg tggcaccggc cggtgagatc tatttcgatg aatggagagg tagttacaga
    15121 atcgaccagt cgaaggataa aaaactccct ggttttgaat tcaatccgcg gagctgcagc
    15181 tttatcacag ccggcgagca taacctcgga cttaaactcg attatgtcag cctggtcagt
    15241 tatggtgatg tcaattacat gacagttccc gattcgacca tagccaacct gactctatcc
    15301 gtcaatttca tgttctatga cgggagcctg acgattatgg ccgattccat cctgaaatca
    15361 aacctcaaag gcctggatgt cactcgaaaa agctacctgg attttcttga ctttaccctg
    15421 ggcagtgaat cgggtgatta taagaaggat atggcgggca ttggccgcaa taaacggctg
    15481 cccgatgtgc ttaaccgcac catgatcctg accgatgtca atctttactg gaattcgttc
    15541 accaattcct atatttcacg ggggcctatt ggggtgatga gcctgggccg ggatcccgta
    15601 aaccgctaca tgaacggctt tgtcgaactg atgcggcgca gaagcggcga tggtttgttt
    15661 gtctacctgg aaatagatga aaaccgctac tatttctttg aataccgcaa cggaattctt
    15721 caggccctgt cgcacgacaa ggagtttgtc aaccggatca atgaagttaa gcccgaaaag
    15781 agatcgatgg tgttgcccgg tgccgatcag aaatatgagt acatggttgg cgataaaagg
    15841 aggatgatcg atttcatccg gaaaatgcaa tcgttaagtt attagttcca ggctatgaca
    15901 ttacaaatca tcctgatcgt tatactggct tacctgatcg gctctgttcc taccgccgtg
    15961 tgggttggaa aattgttttt caggctcgac gtgagagaac acggcagcgg aaatgccggg
    16021 gcaaccaata ctattcgggt gctcggatgg aaagccggtc tgcctgtttt tcttttcgac
    16081 gttttcaagg gctggttcgc ggtcaaactc gccgattttt tgctcaccgg aatgatgaca
    16141 gacgaaaccc ttgtctttct taagatcggc ctggctgccg cggttgtatt gggccatgtt
    16201 tttcccgctt tcgcgggatt tcgcggtgga aaaggagtgg ccacactgct gggtgtgggt
    16261 tttgcactgt ttcccgctgc ggtttggatc gtcctggcta tttttatcat catcctgctg
    16321 actaccggtt ttgtttcgct gggttcaatc atcggatcgc tggtcttccc tttcctggaa
    16381 atatttctct tcggtcagga aaacccctgg ctgatcggat tatcgatcct tgtagcgatt
    16441 tttataccca ttacccatcg gaataatatt aaaagactga taaaaggaaa agagtcaaag
    16501 ttcaggccta cccgttatgg gagtaatgct tcaaaaaaga tgtgaaaaaa ttgttaagaa
    16561 agaaaagcag gggtactgac cctgagattt attttagtta tttttactta attaaccaga
    16621 tgttaaatcc taatacacaa taatatgaaa ttcattgttt cgagttcggt ccttcttaaa
    16681 aacctgtcgg cactcagtgg tgtgatcagt tcgtccaata cccttcccat tctggatgat
    16741 tttttgtttg aactgaagga agatcaactc tttgtgacgg cttccgacct ggagaccacc
    16801 atgaccgtta ctatcccgct cactaaagct gaggagcccg gttctgtgac tatcccggcc
    16861 aagctgcttc tggatacgct gaagacgttt tccgacatac cggtcatctt ttcgatcaat
    16921 cccgaaacgt tgctggtgga actttccgcc ggcgagggaa agtataaact cagcggccat
    16981 agcagcgatg aattcccgca atctccggtg ctggaagcta catctgttct cacagtccat
    17041 tcaggggtcc tggtcgatgc tatcaacaaa acgctctttg caaccggcag cgatgaactc
    17101 cggccggtga tgtcgggcgt ttattgcgag ttgtcgcccg aacatctcac tttcgtggct
    17161 accgatgcgc ataaacttgt gagatacagg cgtatggatg cccattccga ccaggtgagc
    17221 tccttcatcc ttcctaaaaa accattgaac cagattaaag gtatcctggc cggtaaagac
    17281 ctggaagtga atatcgaata ttcccagacc aactccatgt tcacgttcga cagttttaag
    17341 attatctgcc ggctgatcga aggaaaatac ccgaactacg aagcggtcat tcctaccaac
    17401 aatccgaatg tcctgacggt cgaccggggt ttgctcctga atgcgatccg aagagtagcc
    17461 ctatttgcta accagtcgac ccaccagatc cggtttaaga tttcaggtaa agaactggtc
    17521 ctgtctgccg aagatattga cttttcgaac gaagcaaagg aacgactgac ttgcagttac
    17581 gaaggcgaag atatggagat cggattcaac tcgcgctttt tgcaggatat ggtcaccaac
    17641 gtttcttccg agcaggttaa atttgaaatg tcggcaccaa accgcgccgg cctgatcatg
    17701 cccgtcgaaa acgaaaacct tgaagaagag atccttatgc tggtgatgcc cgtgatgctg
    17761 aactagtcgt tggcttaata cgatgaccag ttgaacaatc accaggggtg tttgttatgt
    17821 caaaaaaaca tacaatgaca caggacaaat atatccctga aatgtaccgg atggtggatg
    17881 cgatggacac tccgggcttc gtcaaccttt ttacagcaga tggaattttc agatgggcca
    17941 atatggaacc tgtcgctgga aaagagaata tcaccgcttt tctgaccggt ttttttcaat
    18001 ccatcaaagc catcagccat accgacctcg aatactggca ggttcccgga gtaagattcg
    18061 ctacaggcaa cgtcaattac acccgccgcg acaatagctt gctcagggtg ccgttcagcg
    18121 tgatcctgaa attcaaaggg gatctgatca acgagttc
//
LOCUS       DAOPNH010000025        18115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_2985_length_18115_cov_7.093577, whole genome shotgun sequence.
ACCESSION   DAOPNH010000025 DAOPNH010000000
VERSION     DAOPNH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_18115_cov_7.093577"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..527)
                     /locus_tag="PKZ74_02475"
     CDS             complement(108..527)
                     /locus_tag="PKZ74_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91646.1"
                     /translation="MFRSDSGVSTGDHMQVVLWEDIDGDGDPGTNASLVYSETLTVTT
                     VSDVAWNHFELSQPVRFDGPGDVLIGVVARQVATTQSLYPAALDRGSSQQRSWGGQYS
                     GEVPDPPTLPPDGLWGLMDDFGFPGNWLLRGEGCPVE"
     gene            602..1726
                     /gene="hemW"
                     /locus_tag="PKZ74_02480"
     CDS             602..1726
                     /gene="hemW"
                     /locus_tag="PKZ74_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPM91647.1"
                     /translation="MAGIYIHIPFCKQKCHYCNFFSVASVKWRDEFTRALLKEMELRK
                     DYLQGEPVNTVYFGGGTPSLWSIAHLALCIAHLALHFNIEHGVEITLEANPDDINETS
                     LEEWRKAGINRLSIGIQSFFDDDLRYLNRVHDAGQAHRAIEAARRVGFDNLTIDLIYG
                     IPGLTEEKWAKNLETFFSLDIPHLSAYALTVEQKTPLALLIEKGKYAPVDEELSVRHF
                     KMLLEQAKANDFIHYEISNFAREGYYSKHNSLYWLGGHYLGLGPSAHSFNGHSRQWNV
                     SNISQYIKLDEYQSSVDEKEVLSTDQRYNEYVMTSLRTVWGCDTVHIRNVFGEPFENH
                     FIKKSHLFIKKNHLYREGTKYFLTEEGKLFADGIAAGMFR"
     gene            1772..3310
                     /locus_tag="PKZ74_02485"
     CDS             1772..3310
                     /locus_tag="PKZ74_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPM91648.1"
                     /translation="MPIIGQLVKRAYELGRLPDIAKPKLDPYTAQNRVLKKLLRRAQF
                     TAFGEYYGFSDILKSPDPIKAFQAKVPTFTYNTMFRKWWYRALNGEGFVSWPGRVKYF
                     ALTSGTSEASSKHIPVTSEMIRAIRKVSIRQLVSSLSYHLPDHFYEKGMLMIGGSTHL
                     NFNGTYYEGDLSGISASTIPFWFQHFYKPGKRISRERDWATKLDEIVKSAINWDIGVI
                     VGVPAWIQIILEKIITHYNLKTIHDIWPNLAIYVHSGVSIEPYTKSFEKLFGKPMIFH
                     ESYLASEGYIAYQKNIKVKALQLVLDNGLFFEFVPFDDKNFDDEGNMVDKPVALTIDQ
                     VEEGKEYALLLSTCAGAWRYLIGDTIKFTSIMHSEIVITGRTKHFLSITGEHLSVENM
                     SRAVKMLCDEMNVEIREFTVAGIKHDTMWAHKWYLGTDDPLNAAVAREKIDEALKILN
                     DDYRVERIAAIKDVFVEVLPSAAFSTWMKIHGKEGGANKFPRVLKKDQPAAWEQYLKE
                     YNFK"
     gene            3315..3950
                     /locus_tag="PKZ74_02490"
     CDS             3315..3950
                     /locus_tag="PKZ74_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPM91649.1"
                     /translation="MQTIIEGILLGLTLSIFFGFGPALVAEIQTSILRGFWAAVLLAF
                     GVFLSDAVLVALGFLGAVQIFESNKTILGLVGGIVLIIFGIVTYRRPVLINVDTSDPE
                     YKKQEPFFTTYILKGFFINFTNPFIWIFWMGIVVGFTANYKADMLMLVSFFASALGTV
                     LLFDVLKIFSAYKVKKYLQTHNIVWINRIAGVGLVLFGIYLVVKTYLLIGV"
     gene            4035..4559
                     /locus_tag="PKZ74_02495"
     CDS             4035..4559
                     /locus_tag="PKZ74_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPM91650.1"
                     /translation="MKTSNWFVFLINGLIAILFGVFVLMVSQDMMLKYVRYFGIFILV
                     CGLVLFYFSYRNKKANKSYVLNMVMSIFAFLIGLLIAINPGKSLDLFMTLIGIWAALM
                     GLLQIILSVRMRKKVTNHYLFSINGVITLVFGLLLFYSQMGTDGTLSKVIGVLAVVAG
                     VFLIYLSTKVKAIK"
     gene            complement(4561..6315)
                     /gene="recJ"
                     /locus_tag="PKZ74_02500"
     CDS             complement(4561..6315)
                     /gene="recJ"
                     /locus_tag="PKZ74_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPM91651.1"
                     /translation="MEKRWVLKPQGNKDLVRHLSKVLNINENLANLLAQRGVASYDGA
                     KAYFRPQLEHLHDPFLMKDMHKAVERIEKALKKGEKILVYGDYDVDGTTAVALVYTFL
                     KKLHEKVDFYVPDRYSEGYGISFKGIDFAAENGFSLIIALDCGIKAVEKVAYATGKKV
                     DFIICDHHRPGDSLPEAIAILDPKRDDCTYPYKELAGCGIGFKLIQAFAWKNNIAFSS
                     LEEYLDLVVVSIASDIVPITGENRVLAYYGLKRINSRPRAGLEAILNVSNKYSKTNGE
                     GPDRQYIRELTINDLVFIVGPRINAAGRIKSARTSVELLITKDPAEAFELGNQINKQN
                     SDRKTLDTQATHHALEIIHSDPKFLKRRSTVLYHPEWHKGVIGIVASRLIEAYYRPTI
                     VLTHSEGMITGSARSIKGFDIYDAVDSCGDLLEHFGGHMYAAGLSLLPENFDKFKERF
                     EEYVSTHLTEELAVPEIEIDSKLNLQDVQRKFYDILRQFAPFGPGNMSPVFQTDNVID
                     AGKAKIVGNNHLKLEVFQRDVRSEPISAIAFGQGEENFEHIRDGNPFDICYHIEENEW
                     NGRKSFQLNIKDIKWSAG"
     gene            complement(6423..7673)
                     /locus_tag="PKZ74_02505"
     CDS             complement(6423..7673)
                     /locus_tag="PKZ74_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91652.1"
                     /translation="MKIFLKLLMESYLFAVTEIIRNKVRTLLSLLGITIGIFSIISVF
                     TIFDSMESSIRSSINSLGNDVLFVQKWPWAMGGDYPWWKYISRPEASLTDLKEIQRRS
                     QASETSSYLSGLSETIKYREKSIQNANVIMVAENYDRVMTVDITEGRFISPLEFSSGR
                     PVVVIGSDIAENFLSSTTASALGKKIRIFGMNVEVIGVMKKQGNNMFGNSSDDQLIVP
                     VNFARNHVDMRYAQGAIIVKARTGVSLDQLRDELQGIMRAVRKLKPKAEDNFAINQTN
                     VISKGFDQLFGVIGVVGWIIGGFSLLVGGFGIANIMFVSVKERTRIIGIQKSMGAKNY
                     FILLQFLFEAVFLSLLGGLLGLLIVFLLTFVISRSFDMELVLTVGNIILGITVSALIG
                     FVSGLVPAWNASRLDPVEAMRSTF"
     gene            7775..9256
                     /gene="purH"
                     /locus_tag="PKZ74_02510"
     CDS             7775..9256
                     /gene="purH"
                     /locus_tag="PKZ74_02510"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPM91653.1"
                     /translation="MERKIRSALISVYYKTGLEDILHELRRNGVTIYSTGGTYDFITG
                     LGIPAITVESLTAYPSIFGGRVKTLHPKVFGGILHRRENEEDVKQAKNYNIPPIDLVV
                     VDLYPFRETVARTTDEDEIIEKIDIGGISLIRAAAKNYEDVLIISSVNQYPDLLKILK
                     DQDGVTTLEERRRFAGMAFGVTSSYDMAILGHFQPPVKLRYGENPHQQGTFHGDPGRV
                     FTQLHGKELSYNNLGDIDAAIALIKEFDEPTFAILKHNNACGCASRPVLADAWRKALA
                     GDPLSAYGGVLITNREVDEATAEEIDKLFFEVILAPSYKKSALEKLQSKKNRIILRQG
                     SYDFPERQFRSLLNGIIEQDKDLKTETRDDLRPVTERVPTERETDDLLFANKIVKHTR
                     SNAIVLAKDRQLIGSGVGQTSRVDALKQAIAKAGEFGFDLQGAVLASDAFFPFADSVE
                     IAHKAGITAVIQPGGSVRDQDSVDFCRNHGMAMVFTGTRHFKH"
     gene            9288..10310
                     /locus_tag="PKZ74_02515"
     CDS             9288..10310
                     /locus_tag="PKZ74_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPM91654.1"
                     /translation="MGLFSFLTKEIAIDLGTANTIIIYNDKVVVDEPSIVALERNTGK
                     VIAVGKKAMMMHGKTHENIRTIRPLRDGVIADFKAAEIMIREMIKMIGFKNNVFPPAL
                     KMVICIPSGITEVEERAVKDSAEQAGAKEVRLIHEPMAAAIGIGIDVLEPTGNMIIDI
                     GGGTSEIAVIALGGIVNNKSIRIAGDDFNADIEEYMRKQHNINIGERTAERIKIEVGA
                     ALTDIDNPPDDFPVHGRDMLTGIPKEITVNYAEIAHSLDKSISKIEAAVLNALEMTPP
                     ELSADIYRTGIYLAGGGSLLRGLDKRLHLKTKLPVHMAEDPLRAVARGTGIALKNFDK
                     FTFLIK"
     gene            10312..11133
                     /gene="mreC"
                     /locus_tag="PKZ74_02520"
     CDS             10312..11133
                     /gene="mreC"
                     /locus_tag="PKZ74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPM91655.1"
                     /translation="MRNLLAFISRYQFLFFYLIFLVISFTLLYNNNYYQRSKVVGVTN
                     GITGKFNTYYENMTSFIDLKRSNDELAKENARLRALLNLKTRIIPDSLVRLENDLTEK
                     YISARIISNSTQYRNNFFMIDKGYTSGIKKDMGVIAGNGVVGIIIDVSEHYSSGISVL
                     HKDSRISGRIKKNDQLVNVSWDGVNYRLGDISHIPTHVSLLPGDTILTSGNSRIFPEG
                     LMIGTVEYVDDEMENMFKKGKVRFTVDYNKISYVYVVDNPTRGELNRLQTKTPNE"
     gene            11126..11641
                     /gene="mreD"
                     /locus_tag="PKZ74_02525"
     CDS             11126..11641
                     /gene="mreD"
                     /locus_tag="PKZ74_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPM91656.1"
                     /translation="MNKKLIGNIARFVVLALAQVFIFNKVMVSGYVNPMVYVLFILML
                     PYDIKGFQLLLLAFLMGLTIDLFMHTPGMHALASVTLAFLRPGVIRLVGKKDDVEPWQ
                     YPNVRDSGAAWFLAYTVILVFLHHLMIFYLEVFRLDEFLRTFVKVLINTALSTLIIMM
                     LQYLFYSRKVE"
     gene            11901..13028
                     /locus_tag="PKZ74_02530"
     CDS             11901..13028
                     /locus_tag="PKZ74_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM91657.1"
                     /translation="MFRNSVIFISIVALLASCKPEVKNAFVIDGTVDTTVSGYVLLQK
                     RVEGPMVTVDSVQLTEKGTFRFEGTVGYPEVYYLNIPSTRSSVPFFIEPAPIIVNIIT
                     KDIDKTKITGSKTQTEYDAYLDKLDLFSKKIRDNYQLYNMAQEAGETEKVKYFDSLIT
                     VLDNERTQFSKDYVLQNKSSFISPYIIYRNSYAYELEELETALGGFDTALSHSVYTGF
                     AKDYLATLKRVAIGKPYVPFSMPDSAGTAVALSDQVGKGYLLVDFWASWCAPCREENP
                     NLVKLYSEYHERGFDIFGVSFDTKRDRWLQAIEDDGLTWGHVSDLKGWENAAGKLYGI
                     RSIPSNVLLDSNGVIIAKNLRGDDLRARLAEIWPEPVKGRK"
     gene            14042..17998
                     /locus_tag="PKZ74_02535"
     CDS             14042..17998
                     /locus_tag="PKZ74_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM91658.1"
                     /translation="MTRVKDQKTCTACAAFASVASLEAAINLYANYQFDSIEDTRFSE
                     RHAFNCSKYKSGITTVGCNCDEGKGISLIYDFFKDIAIVDSDCYPYESPICEDTGASC
                     EIVAQQKCDTPSMTAQIWSYKRYQITQGDSIQRADSLKVKIMTDGPLSVTLKNWPGPD
                     SAQHAVSLIGFETKNDTLYWIYKNSWDTTFGDKGYGKEPFWIGTGAPSYIPSIGSSVY
                     AINYDDTCSYPPITVICHRNCVTPNFTYYVHDYDKDKDGFFNWGIGQRPDTYPCSQEE
                     DSNDDDNRIGPYDDNYSGLAIMPDIKVKQGTSQWGGAFENGAVINISVDTLYDSTFVF
                     QIGNAGTAQLNLVQYQLSDSGVVTIDYQYPEGQFSLVDLPHKFVCMSDTSTFKIKMFH
                     GAAAGALAHVHIYLDEPDMDSIFEFTIVYNGCETSSGYDSITSEIEWNDTCRAQCRDL
                     HIMRTGELTVLGMVRLSPEADIIVEPGGKLIVDGGCLTRACNSQLWKGIQVWGDSSLS
                     QYPDTNQGFVSVVNGGQIEYAQGGIFAGRADDGDTLYAYSGGTIYAEKAVFLNNVIDV
                     EFLPFINRTPGNQEMENLSHFRDCIFRTHDPDGLIEKVSAHAILDGVNGVDFYTSTFE
                     MEETEGNPFETDGKGIGIWATDAEFNVRGMCDTNTIPCIHYDSCYFKNLRYGILATNS
                     GGNNYVWVTETVFDNNIAGIYLSGYHQPSVYSSRFYTCTEQGPPGDLDDAFLGGLYLE
                     GSTGYQVEENYFAGPLTTMPDSGSIATIGIYIKDSGDDENEIYNNYFTGLDAGIIAEG
                     VNRGDKTGLCLKCNDYRTCLNDMLVIEGDSNSRYLGIKEYQGTDDTLSSALAGNTFTS
                     DVQGLQAVDNGGNKKYYWSYFNDADHINYYHHGQHPFLVTYPSDSNYTDNTITLNNKQ
                     TPYLKEEACPSGISNPELKSIEDPRLSINEADQQLQLLENKFDSLVDGGDTEGLDFEI
                     MTSMPDEALELQQQLLSGSPYLSDTVMKQAIYKENVLPNAMIRDILSVNPQSAKSGEI
                     LDALDSRYDPMPDYMMANIMQGLHQIGALESLESKIGYWQNYRSRAVNRLVREYLSDS
                     TLFNTEDSLIGLYQDENTLQSKYRLAFSYWDDENMEQAQNALSAIPGEFNLSSQEQTV
                     YQNYLDFFNILQLMSDSSLHARQLDFTAVQQLVNIMNTGLPLVSNYARALLIKGRHIE
                     YTESVYFPMVVKKYPSYYSQNSKKEGIYEDNRLFLFPNPCGDYIIAYFNTIEQGHNGN
                     LVLYDLNGKELDRIRLNSTQNQRVINLFDHPNGTYIIRLLVDYQPVSTKLLIKGRK"
BASE COUNT         4631 a         4425 c         4603 g         4456 t
ORIGIN      
        1 ggccacgaag gggcgccaga cgcaaccggc catgagggcg ccccgcacga cgctcgagcg
       61 ctcgacccgc gaggggacag cctcgactgc cccccagcgc gccacgctca ctccactggg
      121 cagccttcgc cgcgaagcag ccaatttcct gggaagccga agtcgtccat gagcccccag
      181 agcccatcgg gtggcagcgt gggaggatcc gggacctcgc ccgaatactg cccgccccaa
      241 gagcgctgct gagagctgcc ccgatcgagc gccgcggggt atagcgactg cgtggtcgcc
      301 acctgtcgcg cgacgacgcc gatcagcaca tcccccggac catcgaaccg cacgggctgg
      361 gacagctcga agtgattcca cgcgacgtcg ctcacggtcg tcaccgtgag ggtctcggaa
      421 tacacgagac tcgcgttcgt cccgggatcg ccatcgccgt cgatgtcctc ccagagaacc
      481 acctgcatgt ggtcgcccgt gctgacgccg ctgtcgctgc ggaacaacgc agagacggag
      541 tgcagctcga agggaaacgc actgcggatg aggcggatgt acgcggataa ttcgctttca
      601 aatggctgga atctatatcc atataccttt ttgcaaacag aaatgccact actgcaattt
      661 cttctccgtc gcttcggtaa aatggcgtga cgagtttacc cgggcgctat taaaagagat
      721 ggagctccgg aaggactacc tgcagggtga accggtgaac acggtctatt tcgggggagg
      781 aaccccgtcc ctttggtcca ttgcgcattt agcattgtgc attgcgcatt tggcattgca
      841 ttttaacatt gaacatgggg tggaaattac actggaggcg aatcctgatg atattaacga
      901 aacatcgctg gaagagtgga gaaaggccgg tatcaaccgt ttgagcatcg ggatccagtc
      961 gtttttcgac gatgatctgc gctacctgaa ccgggtgcac gacgccggcc aggcgcaccg
     1021 ggccatcgag gcagcccggc gtgtgggttt cgataacctg accatcgacc tgatttacgg
     1081 cattcccggg ttgacggagg agaaatgggc aaagaacctc gaaaccttct tctcactcga
     1141 tattccgcac ctttcggcct atgccctcac ggtggaacag aaaacgccgc tggccctgct
     1201 tatcgaaaaa ggaaaatacg cgccggtcga tgaggagctg tcggtcaggc atttcaaaat
     1261 gctgctggaa caggcaaaag ccaatgattt catccattac gagatatcca attttgccag
     1321 ggaagggtat tattcgaagc acaacagcct ttactggctg ggcgggcact acctcggcct
     1381 gggcccttcc gcccattcgt tcaacggcca ctcgcggcag tggaacgttt ccaacatcag
     1441 tcaatacatc aaactggatg aatatcagtc atctgtggat gaaaaagaag tgctttccac
     1501 cgaccagcgc tacaacgaat atgtgatgac ctcgctgcgg acggtatggg gctgcgacac
     1561 tgttcacatc cggaatgttt tcggggagcc gtttgaaaat catttcataa aaaaatcgca
     1621 tctgtttatc aaaaaaaatc atctttaccg ggaagggacg aagtatttcc ttacggaaga
     1681 agggaagctg tttgccgatg ggatcgcggc ggggatgttc agatgataac attaattctt
     1741 acttttgtct gaccattttg aaattcacca gatgccgata attggacaac ttgtcaaacg
     1801 ggcctatgaa cttggccgac tgccggatat agccaaaccc aaactcgatc cgtacacggc
     1861 gcagaacagg gttcttaaaa aactcctccg gcgcgcccag ttcactgctt tcggagaata
     1921 ctacggtttt tccgacatcc ttaaaagccc cgatccgatc aaagccttcc aggcaaaggt
     1981 gccgacattc acctacaaca cgatgttccg caaatggtgg tacagggcgc tcaacggcga
     2041 aggcttcgtg agctggcccg ggcgggtgaa atatttcgct ctcacatcag gcacttcgga
     2101 agcctccagc aagcatattc ccgtcacttc cgaaatgatc cgggcgatcc ggaaagtcag
     2161 catccggcag ctcgtttcgt cgctttccta ccatctcccc gaccatttct acgaaaaagg
     2221 gatgctgatg attggcggga gtacccacct gaacttcaac gggacctatt acgaaggcga
     2281 cctgtcgggc atctcggcca gtaccatccc gttctggttc cagcatttct acaaacccgg
     2341 aaagaggatc tccagggagc gcgactgggc aacaaagctg gatgagatcg tcaaatcggc
     2401 catcaactgg gatatcgggg tgatcgtggg agtaccggcg tggatccaga tcatcctcga
     2461 aaagatcatc acccattaca acctgaaaac catccacgat atctggccga acctggccat
     2521 ctatgtccac agcggcgttt cgatagagcc ttataccaaa tcgttcgaaa agctgttcgg
     2581 caaaccgatg atcttccatg aatcgtacct ggcgtcggaa ggatatatcg cctaccagaa
     2641 aaacatcaaa gtgaaagcgc tccagcttgt cctcgataat ggcctgtttt tcgagtttgt
     2701 cccgttcgac gacaagaatt tcgacgacga aggcaacatg gtcgacaagc cggtagcttt
     2761 gacgatcgac caggtggagg agggaaaaga gtatgccttg cttttgtcga cctgcgccgg
     2821 cgcctggcgc tacctgatcg gcgacaccat caagtttacc tccatcatgc actcggagat
     2881 cgtgatcaca ggccgtacca agcatttcct gagcatcacc ggcgagcacc tttcggtgga
     2941 aaacatgagc cgggccgtta aaatgctctg cgacgagatg aacgtggaga tcagggagtt
     3001 caccgttgcg ggcatcaagc acgacacaat gtgggcgcac aaatggtacc tgggcaccga
     3061 cgatccgctg aatgcagcgg tagcccggga aaagatcgac gaggcgctga aaatcctcaa
     3121 cgacgactac cgtgtggaac ggattgccgc tataaaagac gttttcgtcg aagtacttcc
     3181 ttcggcggcc ttctccacgt ggatgaagat tcacggtaaa gagggaggag cgaacaaatt
     3241 cccgcgggta ttaaagaaag accagccggc agcatgggaa cagtacctga aagagtataa
     3301 tttcaaataa accgatgcag actatcatag agggcatact tctcgggctc accctatcta
     3361 ttttcttcgg atttggcccg gccctggtgg ccgagatcca gacgagcatc ctgcggggat
     3421 tctgggccgc ggttttgctg gcattcgggg ttttcctcag tgatgcggtc ctggtcgcgc
     3481 ttggtttcct gggagccgtg caaatctttg aaagcaataa aaccattctc ggcctggtgg
     3541 gcggcattgt cctcatcatt ttcgggatcg ttacctaccg gcggccggtg ctgatcaatg
     3601 tcgacaccag cgatccggag tataaaaagc aggaaccgtt cttcaccacc tacatcctga
     3661 aagggttctt tatcaatttc accaacccct tcatctggat attctggatg ggtattgttg
     3721 tcggatttac agccaattac aaagccgaca tgctgatgct ggtctccttt ttcgcgtcgg
     3781 cactgggcac ggtcctgctt ttcgacgtgc tgaaaatttt ctccgcctac aaagtcaaga
     3841 aataccttca aacgcataat attgtgtgga tcaacaggat agccggggtc gggctggtgc
     3901 tgttcgggat ttatttagtg gtgaagacgt accttttgat tggggtgtaa gggttccagg
     3961 ttccaggttc cgggttccag gttccaggtt ctagatttta gattgtaaac cgtaaattgt
     4021 aaattgtaga taatatgaaa acgtccaact ggtttgtttt tctgatcaac gggttgatag
     4081 ccattttgtt cggtgtattt gtcctgatgg tatcgcagga tatgatgctg aaatatgtcc
     4141 ggtatttcgg gatatttatc ctggtctgcg ggttggtcct tttttatttt tcataccgga
     4201 ataagaaagc caacaagagc tacgtgctga acatggtcat gtcaattttt gcattcctga
     4261 tcgggctgct gatcgcgatc aatcccggca agtcgctcga cctgttcatg accctgatcg
     4321 gcatctgggc cgcgttaatg ggtttgctgc agatcatcct gtcggttagg atgcgaaaaa
     4381 aagtaacgaa ccattacctg ttcagcatca acggcgtgat cacgctggtt tttggtttgc
     4441 tgttgtttta cagccagatg ggcaccgacg ggacgttgtc aaaggtgatc ggggttctgg
     4501 cggtcgtggc cggggtgttt ttgatttatt tgtcgacgaa ggtgaaagcg ataaagtaac
     4561 ctaccccgcc gaccatttga tgtctttgat attgagctgg aaggatttgc ggccgttcca
     4621 ttcgttctct tcgatgtggt agcagatgtc gaacgggttg ccgtcgcgga tgtgttcgaa
     4681 gttctcttcg ccctgtccga aagcgatggc tgagataggt tcgctgcgga catcgcgctg
     4741 aaagacctcc agcttaaggt ggttgttgcc gacgatcttg gctttgccgg cgtcgatcac
     4801 attgtcggtc tggaagacag gcgacatgtt gccggggccg aacggggcaa actgcctgag
     4861 aatatcgtag aacttccgct ggacatcctg caggttcagc ttcgagtcga tctctatttc
     4921 cggtacagcc agctcttcgg tcaggtgggt actcacatat tcttcgaagc gttccttgaa
     4981 tttgtcgaaa ttttcgggca ggagcgacag gccggcggcg tacatatggc ctccgaagtg
     5041 ctccaggaga tcgccgcagg aatcgacggc gtcgtagatg tcgaatcctt tgatcgaacg
     5101 ggccgagccg gtgatcattc cctcggagtg cgtcagcacg atcgtcggcc ggtaatacgc
     5161 ttcaatcagg cgcgacgcca caatcccgat cacccctttg tgccattcgg ggtggtacag
     5221 gaccgtactc ctccttttca ggaacttcgg gtcggaatgg atgatttcga gggcatggtg
     5281 cgtggcctgc gtatccaggg tcttgcggtc gctgttctgc ttgttgatct ggttgcccag
     5341 ttcgaaggcc tctgccggat ctttcgtgat gagcagttcg accgaggtgc gggcgctttt
     5401 gatcctgccg gcggcgttga tcctcggtcc gacgatgaac accaggtcgt tgatggtcag
     5461 ttcgcggatg tattgccggt cggggccttc gccgttggtt ttgctgtatt tgttgctgac
     5521 gttgaggatg gcttcgagcc cggcccgggg cctggagttg atccgtttca gtccgtaata
     5581 ggccagcacc cggttctcgc cggtaatcgg cacaatgtcg gaggcgatgc tcaccaccac
     5641 caggtcgagg tactcttcca aggatgagaa tgcaatattg ttcttccagg caaaggcctg
     5701 gataagcttg aaaccgatgc cgcatccggc cagctccttg taaggataag tacagtcgtc
     5761 gcgtttgggg tcaaggatcg caatggcttc gggcagggag tctcccggcc ggtggtggtc
     5821 gcaaataatg aaatcgactt tcttgccggt ggcataggct actttttcca cggctttgat
     5881 accgcagtcg agcgcaatga taagactgaa gccgttttcg gcggcaaaat cgatcccctt
     5941 gaatgaaatg ccatatcctt cactgtaccg gtcgggcaca tagaagtcga ctttctcgtg
     6001 aagctttttc aggaatgtat agaccagcgc caccgcggtg gtgccatcca catcataatc
     6061 gccgtaaacc aggatcttct ctcccttttt cagggctttt tcaatccttt ccacggcttt
     6121 atgcatgtct ttcatcagga aaggatcgtg caaatgttcc agttgcgggc ggaaatatgc
     6181 cttcgccccg tcgtaactgg ccacacccct ctgcgccagc aggttcgcca ggttttcatt
     6241 aatattcaaa accttgctca gatgcctgac cagatctttg tttccctgcg gttttaatac
     6301 ccagcgtttt tccataattt taactggcta aaattaatca tttatctgct gattaatcag
     6361 ctttttgggt gaaaaatacc cggtcggccg gagaaaaatt tttcctccgc tctgaaagcc
     6421 tctcagaatg tggatcgcat ggcttcgacc ggatcgagcc gggaagcatt ccaggccggc
     6481 accaggccgg aaacaaatcc gatcaaagcg gaaacggtga taccaagaat gatatttccg
     6541 acggtgagca ccagctccat atcgaacgac ctggagatga cgaatgtcag caggaaaacg
     6601 atcagaaggc cgagcaatcc gccaagaagc gacagaaaaa ccgcttcgaa caggaattgc
     6661 agcagaatga aatagttctt tgcccccatt gatttttgta ttccgatgat tcgcgtccgt
     6721 tctttaaccg atacaaacat gatattggcg ataccgaacc cacccaccag cagcgaaaat
     6781 cccccgatga tccacccgac cacgccgatc acgccgaata actggtcgaa tcctttcgag
     6841 atcacattgg tctggttgat tgcgaaatta tcctccgctt tcggcttgag ctttctcacc
     6901 gcccgcatga tcccctgcag ctcgtccctc aactggtcga gcgatacgcc cgttctggcc
     6961 ttaacaatga tcgcaccctg ggcataccgc atatccacat gattccgggc gaaattcacc
     7021 ggcacgatca actggtcgtc ggaggaattg ccaaacatgt tgtttccctg ttttttcatc
     7081 acgccgatca cttcgacgtt catcccgaaa atcctgatct ttttccctaa agcggatgcc
     7141 gttgtcgacg acaggaaatt ttcagcgata tcgctgccga taaccacaac cggccttccg
     7201 ctggaaaatt caagcgggga gataaaccgt ccttcggtga tgtcgacagt catcacccgg
     7261 tcgtaattct ccgccaccat gatcacattg gcattctgga tggatttctc gcggtatttg
     7321 atcgtttccg ataatccgga aagatatgag ctggtctcgc tggcctggct gcggcgctgg
     7381 atctctttca gatcggtaag cgacgcttcg ggccggctga tatacttcca ccagggataa
     7441 tcgcccccca ttgcccaggg ccatttctgg acaaacaaca catcgttacc gagagagttg
     7501 atacttgagc ggatggatga ttccatcgaa tcgaaaatcg tgaataccga gatgatggaa
     7561 aatataccaa tagtgattcc caataacgaa agcaatgttc tgaccttgtt ccggatgatc
     7621 tccgtaacgg cgaacaaata gctttccatc aatagtttaa ggaagatttt catctttttt
     7681 gtccccttag agatggtaaa aataatataa atgagatgag attaagcctt atatttgaag
     7741 tttcatcctt aaagaaaaaa tattcgtttt tattatggaa agaaagatca gaagcgccct
     7801 gatttcagta tattacaaga ccggcctcga ggatatcctg catgagctgc gccgcaacgg
     7861 cgttacgatt tactccaccg gcggaaccta tgatttcatt accgggctgg ggattccggc
     7921 cattacggta gaatcgctca ccgcttaccc ctctatcttc ggagggaggg taaaaacgct
     7981 tcatccgaaa gttttcggcg gtatactgca tcgccgtgaa aatgaggaag atgtgaaaca
     8041 ggcgaagaat tacaacattc cacccatcga cctggtggtg gtcgacctgt accccttccg
     8101 ggagacggtg gcccggacga ccgacgagga cgagatcatc gaaaagatcg atatcggggg
     8161 catttcgctg atccgggcgg ccgcaaaaaa ttacgaggac gttctcatca tctcctccgt
     8221 aaaccagtat cccgaccttc ttaagatcct gaaagaccag gatggcgtca cgaccctgga
     8281 ggagcggcgg cgctttgccg gcatggcttt cggtgtgaca tcatcgtacg atatggccat
     8341 cctgggccat ttccagcccc cggtgaagtt gcgttacggc gaaaatccgc accagcaggg
     8401 gactttccac ggcgatcccg gccgggtttt cacccagttg cacgggaaag agctctcgta
     8461 caacaacctg ggcgacatcg atgccgccat tgccctgatc aaagagttcg acgagccgac
     8521 gtttgccatc ctcaagcaca acaatgcctg cggatgcgcc agccggcctg tcctggccga
     8581 cgcctggcgg aaggcgctgg ccggcgaccc cttatcggct tatggcggcg tgcttatcac
     8641 taacagggag gtggatgaag ccacagccga agagatcgac aagctgtttt tcgaggtcat
     8701 cctggcgcct tcgtacaaaa aaagtgcgct ggaaaagctt caaagcaaga aaaatagaat
     8761 aattttgcgc cagggaagtt acgattttcc tgagcgccag ttccgttcgc tgctgaacgg
     8821 gatcattgag caggataaag accttaaaac ggaaacgcgg gatgatctcc ggccggtcac
     8881 cgagagggtg ccgacggaga gagagaccga cgatctgctc ttcgccaaca agatcgtaaa
     8941 gcacacccgc tccaatgcca tcgtgctggc caaagaccgg caactgatcg gcagcggggt
     9001 cgggcagacc tcgcgggtcg atgcgctgaa acaggccatt gccaaagcgg gggaattcgg
     9061 cttcgatctg cagggcgccg ttctggcctc ggacgcgttt tttccctttg ccgactcggt
     9121 ggagatcgca cacaaagccg ggatcacagc cgtgatccag cccggcggtt cggtcagaga
     9181 ccaggattcg gtcgattttt gccgcaacca cgggatggcc atggttttca cgggtacgcg
     9241 gcattttaaa cattaggagt atttacagcg cataacagat agaacgaatg ggactctttt
     9301 catttctcac caaagaaatc gccatcgacc tcggtactgc caacaccatc atcatttaca
     9361 acgataaggt cgtcgttgac gagccctcga tcgttgccct tgagcggaac acaggcaaag
     9421 tgatcgctgt cgggaaaaaa gccatgatga tgcatggcaa gacgcacgag aacatccgga
     9481 cgatcaggcc gttacgcgac ggcgtgatcg ccgacttcaa ggcggccgag atcatgatcc
     9541 gggaaatgat caagatgatc ggcttcaaga acaacgtgtt ccctccggcg ctgaagatgg
     9601 tcatctgcat cccgtcgggg atcaccgaag tggaggaacg ggccgttaaa gactccgccg
     9661 agcaggcggg ggccaaggag gtcaggctga tccacgagcc catggcggcg gcgatcggga
     9721 tcgggatcga cgtgctggag cccaccggca acatgatcat cgacattggc ggcggtacca
     9781 gcgagatcgc ggtcatcgcc ctcggcggga tcgtgaacaa caagtcgatc cgcatcgccg
     9841 gcgacgactt caacgccgat atcgaagagt acatgcgcaa acagcacaac atcaacatcg
     9901 gggagcggac ggccgagcgg atcaagatcg aagtcggcgc cgcgctgacc gatatcgaca
     9961 atccgcccga cgatttcccg gtacacggcc gcgacatgct gaccggcatc ccgaaggaga
    10021 tcacggtgaa ctacgccgag atcgcccact cgctcgacaa atcgatctcc aagatcgaag
    10081 cggcggtgct caacgccctc gagatgaccc cgccggaact ttcggccgac atttacagga
    10141 caggcatcta tctcgccggc ggcggttcgc tgctccgcgg gctcgacaag cgcctccacc
    10201 tcaaaaccaa gctgccggtc catatggcgg aagatccgct gcgggccgta gcaaggggaa
    10261 caggcatcgc gttgaagaac ttcgacaaat tcacattcct cataaagtaa gatgcgaaac
    10321 ctgctggcat tcatttccag gtaccagttc ttattttttt acctgatctt cctggttatc
    10381 tccttcacgc tcctttacaa caacaactat taccagcgtt cgaaggtcgt cggcgtcacc
    10441 aatggcatca ccggaaagtt caacacctac tatgaaaata tgaccagctt catcgacctg
    10501 aaaagatcga acgacgagct ggcgaaagag aatgcccggc tccgggccct gctgaacctg
    10561 aaaacgcgga tcatccccga ttcgctggtg cggctggaaa acgacctgac cgaaaaatat
    10621 atcagcgccc gtatcatcag caactccacc caataccgca acaacttttt catgatcgac
    10681 aaaggctaca ccagcgggat caaaaaagac atgggggtga ttgccggcaa cggcgttgtg
    10741 gggatcatca tcgatgtgtc ggagcactac agctcgggta tctcggtcct ccacaaagat
    10801 tcccggatca gcggaaggat caaaaagaac gaccagcttg tgaatgtctc ctgggacggc
    10861 gtaaactacc ggctgggcga catttcgcac attccgacac acgtgagcct gctgcccggc
    10921 gacacgatcc tgaccagcgg caattcgcgg attttccccg aagggctgat gatcggaaca
    10981 gtggaatatg ttgacgacga gatggagaat atgttcaaga agggaaaagt caggtttacc
    11041 gtcgattaca acaagatcag ttatgtttac gtggtcgaca atccgacgcg gggagagctg
    11101 aaccgcctgc aaaccaagac gccgaatgaa taagaagctg atcggaaata tcgcccgttt
    11161 cgttgtgctg gcgctggcgc aggtcttcat cttcaacaag gtgatggtca gcgggtatgt
    11221 caacccgatg gtgtacgttc tgttcatcct catgctccct tacgacatca agggatttca
    11281 gttgttgctc ctggctttcc tgatgggcct gacgatcgat cttttcatgc acacgccggg
    11341 gatgcacgcg cttgcgtcgg ttacccttgc cttcctcagg cccggggtga tccggctggt
    11401 tgggaaaaaa gacgatgtgg agccgtggca atatccgaat gtacgtgact cgggggcggc
    11461 gtggttcctg gcctacacgg tgatcctggt tttcctgcac cacctgatga tcttttacct
    11521 ggaggttttc aggctggacg aatttttgcg tacctttgtc aaggtgttga taaacacggc
    11581 attgagcact ctgatcatca tgatgctgca atatttgttt tactcccgga aggtggagta
    11641 agagattgtt cttagttttg ttattgttgg aacgaatttc gtgaagctgt attaaaagtt
    11701 gaatggttgc tttttaggtt tgttgtaagg atgtctcaaa agtccatata ttcacttttt
    11761 ttaggtagtc cgacgactga agccgtcggt tcaatcaatt ccggaaccaa cggctttagt
    11821 cgttggagca aaatcacagg caaccaacgg ctttagtcgt tggagcaaca aaatttgtat
    11881 aataaattaa tatttctttt atgtttagaa actctgtaat cttcatttcg attgttgccc
    11941 tcctggcctc ctgcaagccg gaggtcaaaa atgcctttgt catcgacggt actgtcgata
    12001 ccacggtatc cggttacgtt ttgctgcaga aaagggttga aggcccgatg gtgacggtcg
    12061 attccgttca gctcaccgaa aagggcacgt tccggtttga agggacggtc ggttaccccg
    12121 aggtttatta tctgaacatc ccgtcgaccc ggtcgtcggt gccgtttttc atcgagcctg
    12181 cgccgatcat cgtaaacatc atcacgaaag atatcgacaa gacgaagatc accggatcga
    12241 agacgcagac ggagtacgat gcatacctgg ataagctgga tctgttcagc aaaaagatca
    12301 gggacaatta ccagctctac aacatggccc aggaggcggg tgagacagag aaggtgaaat
    12361 atttcgattc gctgatcacg gttctcgata acgagcggac gcaattctcg aaagattatg
    12421 tattacagaa caaatcctca ttcatcagcc cgtacattat ttaccggaat tcgtacgctt
    12481 atgagctgga agagctggag acggccctgg gcgggttcga cacggcgctg tcgcattcgg
    12541 tgtacaccgg ttttgcgaag gattacctgg ccacgctgaa gcgggtggcc atcgggaagc
    12601 catatgtgcc gttctctatg cccgattcgg cgggaacggc ggttgcatta tccgaccagg
    12661 tagggaaagg ctaccttctg gtcgatttct gggcatcgtg gtgtgctccg tgccgcgagg
    12721 agaacccgaa cctggtgaag ctttacagcg aataccatga gagagggttt gatattttcg
    12781 gcgtttcatt cgacacgaag cgcgaccgct ggctgcaggc cattgaagac gacggattga
    12841 cctgggggca cgtttcggat ctgaaaggct gggagaacgc ggcgggcaag ctttacggca
    12901 tccgctctat cccgtcaaac gtattgctcg acagcaacgg cgtgatcatt gcaaagaacc
    12961 tgcgtggcga cgacctgcgg gccaggctcg cggagatctg gccggagccg gtgaagggga
    13021 ggaagtgatt catggacgat tgaacatgga cgcgtgcccg cttgcttggt cattagctcg
    13081 ctgcactcgc gtcattggtg gtcatttatt ggaacagtgg aacagtggaa ctttggaacc
    13141 cggagccctg cagcttcacc gtaggtgatc caatattgta gcagaaaata tttcaaatcc
    13201 cttgcttttc cttccggaat aacatatctt tatcgcacca atgtctcccc ccaattatta
    13261 accgattatt cttaaaagta tgaaaagaaa tgttttcaaa tcaggcgcca tagcaggatt
    13321 gcttatggta ttatttttta ctaacaccct gagcgtgaac tcccagacga ttgagatata
    13381 ccaaaccttc acatcagatg ctgaaataca gcctttcacg cccgaagata ctatttatgg
    13441 catatctgtc agcggttcag tgaatctaaa aagtgatacg agcttggtaa ggataatctt
    13501 atcagatata aacggtaata actggatgat ttatgaatcc tatccattga tcgccagcac
    13561 taaagaattt aatttagggg atgaatgtga tgaaacttgt tctctggatg aaacaattcc
    13621 tgtttcgata acgattcaaa tcattgatgc tgaactatat atctcgacct ttaccgtaaa
    13681 tactattcca ggtgaaaatc ctgccctctt acaatatcag gcaaagcgaa gcaaagacct
    13741 ggagaaagtt cagcaaatga atatcaatgt ccaggccaga ggatggaact ggacggctga
    13801 taccaccaat cttgtaaaga tgttttataa agataagatg caaagatttg atgaaaagtt
    13861 taatcttctg ggtttagact attactccgg cgggattttt aaatcgataa ggcacgcaaa
    13921 cattccaaaa tacaccgatt atactatcat tccttcattt gattggcgtc aaaaacacaa
    13981 tgcccatctt tcttcatctc catattatgg tggagattca caagacccta ctaacggatg
    14041 gatgacaaga gtaaaggatc agaaaacttg tactgcttgt gcagcttttg ctagtgttgc
    14101 ttccctggaa gcagcaatca acctttatgc aaattatcaa tttgattcaa ttgaagatac
    14161 aaggttttca gaacgtcatg catttaattg cagcaaatat aaaagtggaa ttacaacggt
    14221 aggatgtaat tgtgatgaag gaaaaggcat ttccctgata tatgattttt ttaaagatat
    14281 tgctatagtt gattcagatt gctatccgta tgaatcgccg atttgcgaag acacaggagc
    14341 ttcgtgtgaa atagttgccc agcaaaaatg tgacacccct tcaatgaccg ctcaaatatg
    14401 gtcgtataaa agatatcaaa taacacaggg cgattctatt caaagggcag attctttaaa
    14461 agtaaaaata atgactgatg ggcctttgtc tgtaactctt aaaaattggc cagggccaga
    14521 ttctgctcaa catgcagttt ctttgattgg ttttgaaact aaaaatgaca ccttgtactg
    14581 gatatataaa aatagttggg atacaacctt tggtgataag ggttatggaa aagaaccatt
    14641 ctggattggt accggagcgc cttcatatat accaagtatt ggaagttctg tatatgccat
    14701 taattatgat gatacctgct cctatcctcc gattacggtt atttgtcatc gcaattgtgt
    14761 tacaccaaat tttacctatt atgtccatga ttatgacaag gataaagatg gattttttaa
    14821 ctggggaata ggacaaagac cagatactta cccgtgcagc caggaggaag acagtaatga
    14881 tgatgataat aggattgggc cttatgatga taattattcg ggcttagcta tcatgcctga
    14941 tattaaggta aaacaaggta cgagtcagtg gggaggagcg tttgaaaatg gtgcggtaat
    15001 aaatatcagc gttgatacgc tgtatgattc aacatttgta ttccagatcg gtaatgctgg
    15061 aacagcacag ctaaatcttg ttcaatacca actatcggat agtggcgttg ttaccattga
    15121 ttatcagtac cctgaaggac agtttagtct tgtcgacttg cctcataaat tcgtttgtat
    15181 gagcgatacc tctaccttca aaatcaaaat gttccatggc gccgcggccg gagcgctcgc
    15241 tcatgtgcat atttatctcg atgaacccga tatggattct atctttgaat ttacaattgt
    15301 ctataatggc tgtgaaacct catcaggtta tgattccatc acttctgaaa ttgaatggaa
    15361 cgacacctgc cgtgcgcaat gcagagacct tcacattatg agaactggtg agcttaccgt
    15421 tttgggaatg gtaaggctta gccctgaagc tgatattatc gttgaaccgg gaggaaaact
    15481 gatcgtcgat ggcggatgcc tgacccgagc ctgtaacagc cagttatgga aaggcatcca
    15541 ggtatgggga gatagttcgc tgtcacaata tcccgatacc aaccagggat ttgtcagcgt
    15601 ggtcaatggc gggcaaatcg agtatgccca gggtggcatt tttgccgggc gggcggatga
    15661 cggtgatacg ctttacgcct actcaggcgg tacgatctat gccgaaaaag cggttttcct
    15721 gaataatgtc atcgacgttg agtttcttcc gtttatcaac aggaccccgg gcaaccagga
    15781 aatggagaac ctgagccatt tcagggattg tatcttccga actcatgatc ctgacggtct
    15841 cattgagaaa gtcagtgcac acgccatcct ggatggggtg aacggggtgg atttttacac
    15901 aagcaccttc gaaatggaag aaaccgaggg caatccgttt gaaaccgatg gaaaagggat
    15961 cgggatatgg gcaacggacg cggagtttaa cgtccggggc atgtgcgata cgaacaccat
    16021 tccttgtatt cattacgatt catgctattt taaaaacctg cgctatggca ttttggccac
    16081 caattcggga ggaaataact atgtgtgggt gaccgagacg gttttcgaca acaacatcgc
    16141 tggaatttat ttatcgggtt atcatcagcc ttctgtttac tcttcccgtt tttacacctg
    16201 cacggaacag gggcctcccg gggacttgga tgacgcattc cttggcggtt tgtacctcga
    16261 aggctccacc gggtaccagg ttgaggagaa ttattttgcc gggccattga ccacaatgcc
    16321 cgacagcggt tcgattgcca cgattggcat ttatatcaag gattcaggcg acgatgaaaa
    16381 cgagatatat aacaactatt tcacagggct ggatgcaggt attatcgcgg aaggggtaaa
    16441 tcggggcgat aaaaccggcc tttgcctgaa atgcaacgat taccgcacct gcctgaacga
    16501 tatgctggtg atcgaaggtg acagcaacag caggtacctt ggaatcaaag aataccaggg
    16561 cactgacgat acgttaagtt cggcactggc agggaatact tttacatcgg atgttcaggg
    16621 actgcaagca gtggataatg gcggaaataa aaaatactac tggagctatt ttaatgacgc
    16681 tgaccatatt aattattacc atcatggaca acatcctttt cttgtaacct atccttctga
    16741 tagtaattat acagataata caattacatt gaataataaa caaacacctt atctgaaaga
    16801 ggaagcctgt ccatcaggaa tatcaaatcc agaacttaaa agtattgaag atccacgatt
    16861 atcaatcaac gaggcggatc agcaattgca attacttgaa aataaatttg attcattggt
    16921 tgatggagga gatacggaag ggctggattt cgagattatg accagtatgc cggatgaggc
    16981 gctggaactt cagcagcagt tgctttccgg gtcgccctat ctctcggata cggtaatgaa
    17041 gcaggctatt tataaagaga atgtccttcc caatgcgatg atcagggata tcttatctgt
    17101 aaatccgcag tcggcaaaat ccggtgaaat ccttgatgca ttagactccc ggtacgatcc
    17161 catgccagat tacatgatgg ccaatatcat gcaagggttg catcagatcg gtgccctgga
    17221 aagccttgaa tcgaagatcg gatactggca gaattaccgg tcaagagcag ttaacaggct
    17281 ggtcagagaa tatttgtccg actcgacttt attcaatact gaagacagcc tgattggttt
    17341 ataccaggat gaaaacacac ttcagtcaaa atatcgcctt gctttttctt attgggatga
    17401 tgaaaatatg gaacaggctc aaaacgcgtt atctgcaatt cctggtgaat tcaatttatc
    17461 ttcccaggaa cagaccgttt accagaatta tcttgacttt ttcaatattc tccaattgat
    17521 gagcgatagc agtcttcatg cccggcagct cgatttcact gccgttcagc aactggtaaa
    17581 catcatgaat accggtctgc cgttggtcag caattatgcg cgggcattgt taatcaaagg
    17641 aaggcatatc gaatacacgg aatcggtata ttttccgatg gtagtaaaaa agtacccttc
    17701 atattattca caaaattcaa aaaaagaagg tatttacgaa gataatcgcc tgttcctttt
    17761 cccaaatccg tgtggagatt atattattgc ctatttcaat acgattgaac aaggccacaa
    17821 cggaaacctt gttctgtatg accttaatgg aaaagaatta gacagaatcc gtttaaattc
    17881 tacacaaaat cagcgagtta tcaatctatt cgatcatccc aatggaactt atataattcg
    17941 attattagtg gattatcagc ctgtctccac taagctgctg attaaaggaa ggaaatagga
    18001 ttaattaata atccatttca actctcttat tccaactcat tttatcatct taagtttttt
    18061 atcttagtac atgacaaaaa ttaataacaa cttgaatatc aataatatat gttaa
//
LOCUS       DAOPNH010000026        18001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3007_length_18001_cov_7.367046, whole genome shotgun sequence.
ACCESSION   DAOPNH010000026 DAOPNH010000000
VERSION     DAOPNH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3007_length_18001_cov_7.367046"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="rodA"
                     /locus_tag="PKZ74_02540"
     CDS             complement(<1..882)
                     /gene="rodA"
                     /locus_tag="PKZ74_02540"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPM91659.1"
                     /translation="MTPATKNIYGKIDWTTVWLYIILVLMGWVSVYSSVYDETHSSIL
                     DISQRYGKQMIWIIAGGVIAFVIMLTDAKVFNAFAYIIYGISILILIAVLLIGTEISG
                     SKSWFQIGSVAIQPAEFAKFATCLALARYLSSYATDMNVLQTKIVATALIFTPALLIF
                     LQNDTGSAVVYIALILVLYREGLSGYFLISGVIMVVLFVMTLLINQYIMTGILSVLAV
                     LLYYYNKLLRKRWWIIIGIWLASVAMVFAVDYVFDNFLEEHQKTRINVLLGKESDPKG
                     AGYNVNQSKIAIGSGGLI"
     gene            complement(866..2695)
                     /gene="mrdA"
                     /locus_tag="PKZ74_02545"
     CDS             complement(866..2695)
                     /gene="mrdA"
                     /locus_tag="PKZ74_02545"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPM91660.1"
                     /translation="MRKTLESRKYVFLTLFVIAGAVFTIRLFLLQVVQEKYKLSADNN
                     VLRYVTQYPPRGVVFDRNGKLMVYNEAAYDLLVIPKQVKAFDTTEFCRLLEIPIEEVR
                     LRLKKARSYSMVKTTVFLEQLSKEDFSALDEVLFKFPGFFVQLRTVRKYDTPTAAHVL
                     GYTGEVNNRDIERDPYYKMGDYIGLTGLEKSYETILRGKKGMKIKLVDVHNREMGSYQ
                     DGKYDSAAVAGKNITVSIDAEVQEYAEKLMRFKRGSIVAIEPSTGEILVMVSSPSYDP
                     NLLVGRGRSKNFAEFNKDTLKPLFNRAIQAQYPPGSTFKTVNTLIGLQEGVLHDYTRY
                     GCSGTSTTPIPCSHSHPSPLDLYHAIEQSCNPYFWQVFRSILDQRKFRTMQDSYNYWR
                     EEVMSFGIATILEGDVVDQKSGNLPKDTYYDRYYGKTGWKPLTIRSLAIGQGEVEQTP
                     LQLANVAAIIANRGFYHPPHLLKAVEGDTEPAKKFEKKIPARIASTYYETLIEGMSLV
                     YNGGGGSARYYKIDSVECCGKTGTSQNPHGKNHSVFIAFAPRENPKIAIACVVENSGY
                     GATWAAPIASLVMEKYLKRTIKQTATEERMLNAYLIEGSDPGD"
     gene            complement(2702..3256)
                     /locus_tag="PKZ74_02550"
     CDS             complement(2702..3256)
                     /locus_tag="PKZ74_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91661.1"
                     /translation="MKFSIKIRFILALGFLTAIVSCQRTEEYPVEPRITFLEFEKILN
                     ETDSVFDKGVLKFKFTDGDGDIGLSEGDTLPPFNPGSKYYYNLLIDYYEVRNGAETYV
                     PLTSYNSETGLYDTVYLSARVPLLTPEGSNKAISGEIHDSLFIYNYNSDFDTLFLTFR
                     LVDRALNESNTERTPYIVRSFSPQ"
     gene            3313..4308
                     /locus_tag="PKZ74_02555"
     CDS             3313..4308
                     /locus_tag="PKZ74_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM91662.1"
                     /translation="MMLSEEIKARIFSPGNDQDWNCLALEIFRYQAEENPVYRSFLTY
                     LNVNPDVISHYTDIPFMPVGFFKDHVVVTGDSKSAEKVFTSSSTTGTVPSRHFICDLR
                     LYEESFLKAFRLFYGDVANYRFLALLPGYLERTGSSLVYMLDFLIRMTERNGSGFFLH
                     DHDALEARLLQPVEGTIKTILFGASYALLDFSERHPIDLSHAIIMETGGMKGRRREMV
                     REELHHLLCQRFQSESIHSEYGMTELLSQAYSQGNGVYRAPPWMKVLVRDPNDPLTLL
                     PAGKTGGINVIDLANIYSCSFIAAQDLGRSLADGSFEILGRFDESEVRGCNLLVV"
     gene            complement(4243..4722)
                     /locus_tag="PKZ74_02560"
     CDS             complement(4243..4722)
                     /locus_tag="PKZ74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPM91663.1"
                     /translation="MGYCYEYPRPSVSADIAVFRKRGNELQVLLIKRGNEPFKGMWAL
                     PGGFMEMDETLEETAARELEEETGLAGIKLEQFHTFSQVDRDPRTRIVTTVYYGFTNE
                     ENDRAKGGDDADEACWFSTDHLPPMGFDHEMIIRQILNDQKITPSNFTLIKPAQDLK"
     gene            complement(4881..5495)
                     /locus_tag="PKZ74_02565"
     CDS             complement(4881..5495)
                     /locus_tag="PKZ74_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91664.1"
                     /translation="MKTLISTSIFILCLALFSQAKPINEIYSIQDPVETEESYVDDIP
                     FDTWEIAVESILDGDEVKLQEEPYVNDIPFDTRTIANEYLLRKMVETAGERNVNDIPF
                     NTEKITNQYLTGALTREYLNEPNVEDFPENIKSTTCYYEYPCTYTQTVTVKMKPQKRG
                     IYRDKKNGKYDYTIIHPVMIRVNERDLKNPADTDVILMIPGSSL"
     gene            complement(5658..6326)
                     /gene="rsmI"
                     /locus_tag="PKZ74_02570"
     CDS             complement(5658..6326)
                     /gene="rsmI"
                     /locus_tag="PKZ74_02570"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPM91665.1"
                     /translation="MAKLFLVPTPIGNIRDITLRAIDVLQQADLILAEDTRKTAILLK
                     HYGIEGKVGSHHQHNEHRTVKAIADRIANGENIALVTDAGTPGISDPGFLLVRECIRN
                     GVDVECLPGATAFVPAIINSGLPSDRFLFEGFLPMKKGRKTRMEELSKAGCTIVIYES
                     THRILKTLDELASVFGPERPASVSRELTKIYEETVRGTLAEIRQYYQDHPTKGELVIV
                     IGTP"
     gene            complement(6356..7255)
                     /locus_tag="PKZ74_02575"
     CDS             complement(6356..7255)
                     /locus_tag="PKZ74_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91666.1"
                     /translation="MDKKIIKGLIDDKLEVIQEQFSIIRAYEGKIPVIELDLILGSIR
                     DLYELVLGLQQENMAGTKPEPVIIPEEEQPEEPIIEVTLPTPPEPKPEPSRGTETAGR
                     FASGILSMEPVREESPKPELIRQAPPAYIRPTQHYAEPVAVETKVAPDPEPEPFINKD
                     QTTEAYLPEMAPVADSPSASEDKPAADNFQRTTFDLFSDVPPVTIADRLREGQDKRLA
                     DKLQEKITDLRSAIGINDKFLFINELFDGNMRVYDDIVQKLSSAATIAQIDLLLLDLK
                     IAYNWDSESPTVKKFVDLVRRRF"
     gene            7515..7853
                     /locus_tag="PKZ74_02580"
     CDS             7515..7853
                     /locus_tag="PKZ74_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91667.1"
                     /translation="MKTLKSILVATLVAVTMVGLSNTDGFKIKPKPLKVVNVSFEKAI
                     QCPGLVKAMYEQIDKEELLSNGTLNKYVAEVTYSGTLYRITGTRAQWIWFFRIKIDLP
                     YNDDPGIHSN"
     gene            7976..8560
                     /locus_tag="PKZ74_02585"
     CDS             7976..8560
                     /locus_tag="PKZ74_02585"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPM91668.1"
                     /translation="MFLERSNGNMRSGWIEVICGSMFSGKTEELIRRLKRALIARQKV
                     AIYKPAVDIRFSTNDVVSHDENSIPSTPVEASTQILLYANDYDVVGIDEAQFFDDELV
                     NVCNKLAAMGIRVIVAGLDMDYTGKPFGPIPALLATAEYVDKVHAICISCGGLAHYSH
                     RTSNQEGLVVLGATDRYEPLCRKCYLEKIAAPKS"
     gene            8617..9291
                     /locus_tag="PKZ74_02590"
     CDS             8617..9291
                     /locus_tag="PKZ74_02590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM91669.1"
                     /translation="MKKTIIILTALFMALQQSGCSQSPKQEKGKEPVKGNGPETLVLI
                     KTSMGDITVKLYNDTPKHRDNFVKLIREGWYNDSPFHRIIKGFMIQGGGNKDGRQDPG
                     YTIPAEILPNHFHKKGVLAAARMGDNVNPTKASSGCQFYIVEGNIVNEETLNSFEMRY
                     KTKYNAEQRQAYSTVGGAPWLDGGYTVFGEVVSGIEVVDRIAAVPTGPGDKPVAPVTM
                     SIEILE"
     gene            9341..10363
                     /gene="pheS"
                     /locus_tag="PKZ74_02595"
     CDS             9341..10363
                     /gene="pheS"
                     /locus_tag="PKZ74_02595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPM91670.1"
                     /translation="MQEKISSYLAEIDRFTTTNPEELEQFRIKFISKKGLIPALFEDF
                     KNVAPEMRREMGQALNELKNKAQEKINLLREQIEASATEVKRGTDLTLPVDFARNGTR
                     HPLSITRNEIVRIFSRIGFVVAEGPEVEDDWHNFTALNFQQDHPARDMQDTFFIERNP
                     DIVLRTHTSSVQIRAMENRKPPLREIYPGRVFRNEAISARAHCIFHQVEGLYVDHDVS
                     FADLKQTLYYFAHEMFGEGTEVRFRPSYFPFTEPSAEMDVTCFVCGGKGCPICKYSGW
                     VEILGCGMVDPNVLENCGIDSREYTGFAFGMGIERITILKYRIQDIRLFFENDVRFLR
                     QFAGVV"
     gene            complement(10375..11832)
                     /locus_tag="PKZ74_02600"
     CDS             complement(10375..11832)
                     /locus_tag="PKZ74_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPM91671.1"
                     /translation="MKRRVFSSVFVFAIIYTIFFQITALAGNDPSSGEPRAKYVFLFI
                     GDGMGQGQVNLAQAYLAALDGRIGLDDLSFTRFPEAGFCNTYAHNRLITCSAAAGTAL
                     ATGNKTNINRISMDPEATMNLTSVAAKAKNKGYKVGILTSVSIDHATPAVFYAHQPDR
                     DLYFDIGRELTQSRFDLFAGGGFVQPDSTNGGQTVNLVSLAAENGFNVADTREEFEKL
                     APGTGKTLILSPRIAGEASMPYYIDMDPGDITLADYTAKAIDLLSGDEGFFMMVEGGK
                     IDWACHANDASTVIQEIIIFSRAIESAVRFYEKHPEETLIIVTADHETGGLALGNRKM
                     KYSSNLELLKYQLSSTDEMNKIVSQFRVNKSGDPDADFLRMMKVVENETGLHSRANGT
                     LLTDSELAHLKERFLESVYAAGPEQGTYIPEDKFIESVIGLRDEKAGISWGSGSHTFV
                     SVPVYAIGQGAEKFSGYIDNTDIPKLIGEIMQLER"
     gene            12014..13288
                     /locus_tag="PKZ74_02605"
     CDS             12014..13288
                     /locus_tag="PKZ74_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPM91672.1"
                     /translation="MQQKLNPQDIEIMAPVGSYESLSAAIQGKADAVYFGIGHLNMRS
                     RSTQNFSLEDLAEIAAICRENHLRSYITLNTEIFDEEISLMRAIVDEAKKAGITAIIA
                     SDQAVIQYARSREVEVHMSTQANITNTEAVKYYAQFADVMVTARELNLDQVKRITENI
                     EGERIIGPSGKLVQIEVFAHGALCMAVSGKCYLSLDNMNSSANRGACLQLCRRPYRVF
                     DRDGEVELEVDNQYIMSPKDLKTIDFIDKILDAGVRVLKIEGRGRSPEYVKTVTRCYR
                     EAVNAHCDGTFNPENLERWNHELGTVYNRGFWDGYYLGRTVGEWAAGYGSQATKKKIY
                     VGKVMNYYARTGIAEVKIEAHPLRKGDHFLVLGPTTGVAEGITPDIWLEEKIVGEVNR
                     GDLCTFPFDTPLRPSDKLYKWVDSREVEGQID"
     gene            13434..14312
                     /locus_tag="PKZ74_02610"
     CDS             13434..14312
                     /locus_tag="PKZ74_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPM91673.1"
                     /translation="MMKYFNLHTHCNYCDGAGFPEEYVNAAIKKQFHTLGFSSHAPVP
                     FKNTFAIRDEQELQKYCDQIIHLKEIYKDKIDIYLGLELDYIEGISGDFGKIKEKYGL
                     DYTIGSVHLVRNGDDENLWFIDGPRVESYDEGLKSVFKGDVKQAVTTYYEQVKKMVVT
                     QKPDILGHFDKIKMHNKDRYFKEDEAWYRDRVMDLLDAISTTGVIPEVNTRGIYKKRS
                     SDLYPGVWILKEMKKMNIPVILSTDAHHPVEIDGYFDETVGILKEIGYNSLVCYNANG
                     WQELPIQIEYRPFACS"
     gene            14759..15187
                     /locus_tag="PKZ74_02615"
     CDS             14759..15187
                     /locus_tag="PKZ74_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91674.1"
                     /translation="MKNLMKTLVLISVLFIAGMQNINAQLSIKVILDGQCPDTCTSHE
                     ICTYKVEYQLYNICVEPPELCCTAPPQWVDCSTEEIYFNCDFSCDDPSNEKCYALVGK
                     ATKYCNGPGGNIAICVGTGIAYYTCHEVMTLSPLPVTVYW"
     gene            16307..>18001
                     /locus_tag="PKZ74_02620"
     CDS             16307..>18001
                     /locus_tag="PKZ74_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM91675.1"
                     /translation="MANVFPNHVLWGFPNILVDYLLRFEWTGEPCQGYPVQFKPNFIP
                     TPQTIQWDFDDGPGSTSWQLSPTYAFKNPGVHEVKVDVWYPSGRYEHTSREIEISPSP
                     QPDLGPDTMICDGASITLNANCEADFFSWSTGQFGVSSITVSDSGNYWVRGRFNESGC
                     EGYDTIHVGFHPPTIIDETNLVIIPTTCNGASGSITGLIALGTPPFAFQWLDLSGNPY
                     GTNIDASGLPAGQYQLTITDGNGCEAVSEVYTIEDAGNLQVLEAELTQPHCGRPDGEI
                     VVHAYSPSGSILEYSINDGADYTSDSVFTGLSAGTYVVRIRDVNGCDGFYLSNPVYLA
                     DIPGPQVVEVNVTDETDFLGNGAIEIVANGSTPLIYYSIDGGATWQVNDGNFENLESG
                     IWNLVIKDENGCDTTFTVEIQNIILTWLHAVTAVGGHCLGNTAMVPVNVDNFNAVADF
                     HLKLGYNADNLECEGFANVHPELADSLSGWVDYLAGDIHLAWNSPASVTFTGTEKVAD
                     LVFTTKNPGQGELAWYTGNAESYFANSSGNPIPAEFSTGEVTVYEPPEIILAQSKTV"
BASE COUNT         4681 a         4290 c         4249 g         4781 t
ORIGIN      
        1 gatcaatcct cccgatccaa tggctatttt cgactggttg acattgtaac ccgcgccttt
       61 cgggtcagat tccttgccca ataaaacatt gatacgggtc ttctggtgct cttccaggaa
      121 gttgtcgaat acataatcca ccgcaaatac catggcaacc gaagccagcc atatgccgat
      181 aatgatccac cagcgcttcc tgagcagctt attgtaataa tagagcagaa cggccagcac
      241 cgacaagatt ccggtcataa tatactggtt gatcagcaag gtcataacaa aaagcacgac
      301 catgattacg cccgagatca ggaaatatcc cgaaaggcct tcccggtaaa gtaccaggat
      361 aagcgcaata tacacaacgg ccgaaccggt atcattttgt aaaaaaatca gaagcgccgg
      421 tgtgaatatc aaagcagtgg ctacaatctt ggtctgaagc acattcatat ccgtggcgta
      481 ggaactcagg tatctggcca gggccaaaca ggtggcgaat ttggcaaatt cggcaggctg
      541 tatggccaca ctgccgatct ggaaccagga tttggaaccc gaaatctcag tgccgataag
      601 aagtacggca atcaggatca gaatggaaat accatagatg atataagcaa aagcattgaa
      661 gaccttggcg tcggtcagca tgatcacaaa cgctatcacg ccgccggcaa tgatccagat
      721 catctgtttt ccgtaacgtt gcgagatatc caggatgctg ctgtgagttt catcatagac
      781 cgatgaatat acactgaccc atcccatcag caccaggatg atgtaaagcc agacagttgt
      841 ccagtcaatc ttgccgtata tgtttttagt cgccggggtc acttccttct attaaataag
      901 cgttcaacat ccgttcttcc gtagcggtct gctttattgt ccttttcagg tatttctcca
      961 tgaccagcga agcaatcggc gccgcccagg ttgctccata tcccgaattt tccacaacgc
     1021 aggctatggc tatcttcgga ttctcccgcg gagcgaaagc aataaacacc gaatggtttt
     1081 tcccgtgcgg attttgtgag gtgcctgtct ttccgcagca ttccacgctg tcaattttgt
     1141 aatacctggc cgatcccccc cctccgttat aaaccagcga catcccctcg atgagcgtct
     1201 cataataggt cgaggcgatc cgggcaggga tcttcttttc gaactttttc gccggttccg
     1261 tatcaccctc aacggctttc agcagatggg gcggatggta aaatccccgg ttggcaataa
     1321 tggccgccac gttggccagt tgcagcggcg tctgttccac ttctccctgc ccgatcgcca
     1381 gcgaacggat cgtcaggggt ttccaacccg ttttgccgta ataccggtca tagtaagtgt
     1441 ctttgggcag gttcccgctt ttctgatcaa ccacatcccc ttccagtatg gtcgcgatac
     1501 cgaaactcat cacctcttcc cgccagtaat tataactgtc ctgcatcgta cggaatttcc
     1561 gctggtcaag aattgaccgg aaaacctgcc agaaataagg attgcacgat tgctcaatgg
     1621 catggtacag gtcaagcggc gaaggatggc tgtggctgca gggaatagga gtggtcgatg
     1681 taccactgca tccgtacctt gtgtaatcgt gcagcacacc ctcctgaagc ccgatcaggg
     1741 tattgacagt cttgaacgtc gatccgggag gatattgcgc ctggatagcc ctgttaaaaa
     1801 gaggtttaag cgtatccttg ttaaactcgg caaaattttt cgaccttccc cttcctacca
     1861 ggaggttcgg atcgtacgac ggactggaga ccatcaccag tatttctccc gtagagggct
     1921 ctatggctac gatactcccc cttttaaacc gcatcagttt ttcagcatat tcctggacct
     1981 ccgcatctat cgaaaccgtt atgttcttgc ctgcaaccgc cgcactgtcg tatttcccgt
     2041 cctggtagct ccccatctcc cggttgtgca catccaccag cttgatcttc atcccttttt
     2101 tgccccgcag gatcgtttca taggatttct ccaggccggt cagcccgatg tagtcgccca
     2161 ttttataata aggatcccgt tcaatatccc tgttatttac ttcaccggta taacccagaa
     2221 catgcgcggc tgtgggagtg tcatacttcc tgacggtacg gagctgtacg aaaaaacccg
     2281 gaaatttgaa caaaacctca tccagcgcgc tgaaatcttc tttcgataat tgttcgagga
     2341 ataccgtcgt cttgaccatc gaataactcc tggctttctt cagcctcagc cttacttctt
     2401 caatggggat ttcaagcagc cggcagaatt ccgtcgtatc aaaagccttt acctgctttg
     2461 ggataacaag caggtcatac gcagcctcat tgtataccat cagcttgccg ttacggtcaa
     2521 aaaccacccc tcggggagga tattgcgtca catagcgcaa gacattgttg tcggccgaga
     2581 gtttgtactt ttcctgcacc acctgcagca ggaatagccg gatggtaaat acagcccctg
     2641 caatcacaaa gagggttaaa aaaacgtact tcctgctttc cagcgttttt ctcatgaccg
     2701 gttattgcgg agaaaaggat ctgacaatgt aaggggtccg ttctgtgttg ctttcgttca
     2761 gagcccggtc aaccaatctg aatgtcagaa aaagggtatc aaaatcggaa ttgtaattat
     2821 aaatgaacag tgagtcatga atttctcctg aaatggcttt gttcgatcct tcaggtgtca
     2881 gcaacggcac cctggcactc aggtaaacag tgtcgtagag accggtctcc gaattgtaag
     2941 aagtcagagg gacataggtt tccgctccat tgcgcacctc ataataatcg atcagcaaat
     3001 tgtaataata tttgcttccg ggattgaacg gcggcaaggt atcgccctcc gaaaggccga
     3061 tatcgccatc accgtcggta aatttgaatt tcagtacccc tttgtcaaaa acactatcgg
     3121 tttcattcag aatcttttcg aattctaaaa atgtaatgcg cggctcaacc ggatattcct
     3181 cggttcgctg acacgatact attgccgtta gaaatcctaa agctaaaata aaacgtattt
     3241 ttatggagaa tttcatcgta tggttgattt ttcaaaatta agcaattttc attcctgaac
     3301 tttttattcc tgatgatgct ttcggaagaa attaaagcac ggatttttag cccgggtaat
     3361 gatcaggact ggaactgcct ggcattggaa attttccgct accaggcgga agagaacccg
     3421 gtttacagaa gttttctaac ctacctgaac gtaaatccgg acgtcatttc gcattacacg
     3481 gacattcctt ttatgcccgt cggttttttt aaagatcatg tggttgtaac aggtgattcg
     3541 aaatctgccg aaaaggtttt taccagtagc agcaccaccg gtacggtgcc atccaggcat
     3601 tttatctgtg atctccggct ttacgaggaa agcttcttaa aagcgttcag gttgttttac
     3661 ggagatgtcg ccaattaccg tttcctggcc ttgttgccgg ggtatctcga acgtaccggt
     3721 tcctccctcg tgtatatgct cgatttcctg atcaggatga cagagcgtaa tggaagcgga
     3781 tttttcctgc acgatcatga tgctctcgaa gcccgtttgt tgcagcctgt tgaagggaca
     3841 ataaaaacaa tactgttcgg tgcatcgtat gctttgcttg acttttccga acgacatcct
     3901 atcgacctta gtcatgcgat cattatggaa acgggtggga tgaaaggcag gcgcagggaa
     3961 atggtcaggg aagagcttca tcatttgtta tgccagcgtt ttcaatcgga aagtattcat
     4021 tctgagtacg gtatgacgga attgctttca caggcttact cgcagggtaa tggtgtatac
     4081 agggcgccac cctggatgaa agtactggtt cgtgatccga atgatccgtt aacgctcctg
     4141 cctgccggaa agaccggcgg gatcaatgtc atcgacctgg ctaatattta cagttgttcc
     4201 ttcatcgcag cgcaagacct gggcagatca ctggcagacg gttcatttga gatcctgggc
     4261 aggtttgatg agagtgaagt tagagggtgt aatcttttgg tcgtttaata tctgtcggat
     4321 gatcatttca tggtcgaatc ccatcggagg aagatggtcc gtactgaacc agcaggcttc
     4381 gtcagcgtcg tcgcctccct ttgccctgtc attctcctca ttcgtaaaac cgtaataaac
     4441 cgtcgtaacg atccgcgttc tcggatcccg gtctacctgg ctgaacgtat ggaattgctc
     4501 cagtttgatc ccagccagcc cggtttcttc ttccagctcc ctggcagccg tctcttccag
     4561 cgtttcatcc atttccataa atccgccggg aagggcccac atccctttga acggttcgtt
     4621 ccctcgtttg atcagcaaaa cctgcagctc attccctctc ttccggaaaa cggcaatatc
     4681 agccgaaacc gacggacgcg gatactcata gcaatagccc ataattatag ttcaaagttc
     4741 actgttcacc gttcactgtt caccgttcac cgtcctaaag tagataattt ttcactataa
     4801 aaagaagcgg ccccgccaga gctgacggag ccatcttcat cacttaacca aaaatgctta
     4861 atgaaatagg acggatataa ttaaagagaa gagcccggaa tcataaggat tacatcagta
     4921 tctgccgggt ttttcagatc ccgctcgttt acccggatca tcacgggatg aattatggtg
     4981 taatcatatt tgccattctt tttatcgcga tagatgcccc ttttttgcgg tttcatctta
     5041 acggttacag tttgtgtata ggtgcaggga tattcgtaat aacatgttgt tgattttata
     5101 ttctccggga aatcctcgac attcggttcg ttaaggtatt ccctggtcaa tgcgccggtc
     5161 aggtattggt tcgtgatctt ttccgtgttg aacgggatat cgttcacatt cctttctccg
     5221 gctgtttcaa ccattttccg gaggagatat tcattcgcga tggtgcgtgt atcaaacgga
     5281 atatcattca catacggttc ttcctgcaat tttacctcat ccccgtcaag aatggattca
     5341 accgcaatct cccaggtgtc gaaaggaata tcgtcgacat aagattcttc agtttcaacc
     5401 ggatcctgta tggagtatat ttcgttaatg ggttttgcct ggctgaacag ggccaggcag
     5461 agaatgaata ttgatgttga aattaaagtt ttcatggctt atatttttat gaaccggatg
     5521 gcaagggttc tcataaagtg tgccacaatc tgtaatattc agaaaaacaa cattttagga
     5581 cgagtaataa gtcgatcatc ggaatagaaa agtgtccagt tagcggacaa aattgtacgg
     5641 gaccgtacaa tttccaatca aggagtgccg atcacgatca ccagctcccc ttttgtcgga
     5701 tgatcctggt aatattgcct gatttcggcc agtgttcccc tgaccgtttc ttcatatatt
     5761 ttggtcagtt ccctggaaac gctggccggc ctttccgggc caaacacgct ggccagctca
     5821 tcgagggttt ttaatatccg gtgagtggat tcgtaaatga cgatagtgca tccggccttt
     5881 gacaactctt ccatccgggt tttccgtcct tttttcatcg gcaggaaacc ttcgaaaaga
     5941 aatctgtcgc tggggagacc ggaattgatt atagccggca cgaaagccgt ggcgccggga
     6001 aggcattcca catcgacgcc attccggata cattccctga ccagcaggaa ccccgggtcg
     6061 gatatcccgg gggtcccggc atccgtaacc agggctatgt tctctccatt agcaatcctg
     6121 tcggcaatgg cctttacggt cctgtgttcg ttgtgctggt gatgcgaacc gactttccct
     6181 tcaatcccgt aatgtttcag cagaatagcc gttttccgcg tgtcttccgc caggatgaga
     6241 tcagcttgct gaagcacatc gatggcacgc agcgtaatat cacggatatt gccgatcggg
     6301 gtagggacca gaaaaagctt tgccatgttg ccagacatta aattcgttac aaggatcaga
     6361 atctccgcct gaccaggtcg acgaatttct tgacggtggg tgattcgctg tcccagttat
     6421 aagcgatttt caagtcgagg agaagcaggt ctatctgtgc tatcgtcgct gcgctgctca
     6481 atttctggac gatatcgtcg taaacccgca tgttgccatc gaacagctca tttataaaaa
     6541 ggaatttgtc gttgatgcca atggcagaac gcagatcggt gattttctcc tgcaacttat
     6601 cggccagccg tttgtcctgc ccttcccgaa gacggtcagc aatggtcacc ggcgggacat
     6661 cgctaaaaag atcgaatgtg gtccgctgga aattgtctgc tgcaggctta tcttcggaag
     6721 cagaaggcga atcagctacc ggcgccatct ccggcagata ggcctcagtt gtctggtctt
     6781 tgttgataaa aggctcaggc tccggatcgg gcgcaacttt tgtttctaca gcgacaggct
     6841 cggcataatg ctgtgtcgga cggatgtaag cggggggcgc ctgccggata agttcgggtt
     6901 tgggagattc ttccctgaca ggttccatgc tgagtattcc cgaagcgaat cgtccggccg
     6961 tttctgttcc tcttgaaggt tccggttttg gttccggtgg agttggtaac gtcacttcaa
     7021 taatgggttc ttcaggctgt tcttcctccg gtatgatgac cggttcaggc ttcgttcctg
     7081 ccatgttttc ctgttgcaaa cccagcacga gctcatacag gtcgcggata ctgcccagta
     7141 tcaggtccag ttcaataacg gggatcttgc cttcataagc ccggataatc gagaattgct
     7201 cctggataac ctccagttta tcatcgataa ggcctttaat gattttttta tccatattac
     7261 cggtcgtatt atctgcaaat gctttgtaat tccgacaata aaggtaagaa gattaattca
     7321 agatgatgac aaattcatca ttatatcctt cattaccatg gcctgaactg catactcatt
     7381 gttttccact aaaaaaaaag atcgcctttt tctgccatgt tcaggatata aggccggcat
     7441 acctttgttt catcaacatt gaaattaagc tctttaaaat tccttaaaac taactttaaa
     7501 aaacggaggt tattatgaaa acgttaaaat caattctggt tgcaactttg gttgctgtga
     7561 cgatggtcgg actttccaac accgatggat tcaagatcaa acccaaaccg ctcaaagtcg
     7621 tcaatgtgtc gttcgaaaaa gcgattcagt gcccgggtct ggtgaaagcc atgtacgagc
     7681 aaatcgacaa ggaagagctg ctgagcaacg gaacgctgaa caagtacgtc gcagaagtga
     7741 catacagcgg caccctttac aggatcaccg gcacacgcgc tcagtggatc tggttcttca
     7801 ggatcaaaat cgacctgccc tacaacgatg atccgggaat acactccaac tgaccggaga
     7861 tcggaaagcg aagaagaagg gtgccctgtt gggtgccctt tctttttaac cgctattttt
     7921 tataattttg aaaaatacaa aatcattacg gactttatat aattcttcat tcaatttgtt
     7981 tttagaacgt tccaacggta atatgcggtc aggctggatc gaggtgatct gcggatcgat
     8041 gttttcgggc aagacggaag agttgatccg ccggctcaaa cgggccctga tagccaggca
     8101 aaaggtggcg atttacaaac ctgccgtgga tatccgtttc agcacaaatg atgttgtttc
     8161 acacgatgaa aattccatac catctacacc ggtggaagct tccacgcaga ttttgctcta
     8221 tgccaacgat tatgacgtgg tgggaatcga tgaagcccag tttttcgacg acgagctggt
     8281 gaatgtctgt aataaactgg ccgcgatggg gatccgtgtg atcgtggcag gcctggatat
     8341 ggattacacc ggcaagcctt tcggcccgat tcccgcactt ctggccacag ccgaatatgt
     8401 ggacaaagtt cacgccatct gcatcagttg cggcggattg gcccactatt cgcaccggac
     8461 cagcaaccag gaaggcctgg tagtactcgg ggccaccgac cgctatgaac ctctttgcag
     8521 aaaatgctat ctggaaaaaa tagctgcgcc gaaatcctga aaatctgtct ctaatttttt
     8581 aattttgcgg cgcttaatca ttttaaagct ttaccaatga agaaaacaat aatcatttta
     8641 actgcattat tcatggcatt acagcaatcg ggctgctcac agagccctaa acaggaaaaa
     8701 gggaaagaac cggtcaaagg taatggtcct gaaacacttg tgcttattaa aaccagcatg
     8761 ggcgacatca ccgtcaaact atataatgat accccgaaac acagggataa cttcgtgaaa
     8821 ctgatccgtg aaggatggta caatgattct ccgttccatc ggatcatcaa aggtttcatg
     8881 atccaggggg gaggcaataa agacggccgc caggatccgg gatataccat tccggctgag
     8941 attctgccga atcattttca taaaaaaggt gttctggctg ctgcacgaat gggcgataac
     9001 gtgaacccga ccaaagcttc ttcgggctgc cagttctata ttgtcgaggg aaatattgtc
     9061 aatgaagaaa ccctcaactc cttcgaaatg cggtataaga ccaaatacaa cgccgaacag
     9121 aggcaggcct acagcaccgt tggcggcgca ccctggctcg acggcgggta cacagttttc
     9181 ggtgaagtcg tctccggaat tgaagtggtt gacaggatcg ctgccgttcc gaccggcccg
     9241 ggcgacaaac ccgtggcgcc ggtgaccatg tcgatagaaa tcctggagta atttaatcag
     9301 gatggcgtcc cacaggacgc catccgcagt ccatactttc atgcaggaaa aaatatcttc
     9361 ctatctcgca gagatagacc gtttcacgac gacaaacccg gaggagctgg agcaattccg
     9421 tatcaagttt atcagcaaaa aaggattgat cccggcgctt ttcgaagatt ttaaaaatgt
     9481 cgccccggag atgaggcggg aaatggggca ggcgctgaat gaactgaaaa acaaagccca
     9541 ggagaagatc aatcttctca gggaacagat cgaggcctct gcaacggaag tgaaacgggg
     9601 caccgacctt accctgcccg tcgattttgc cagaaacggt accaggcacc cgctctctat
     9661 cacccgaaac gaaatcgtcc ggatcttctc ccgcatcggg ttcgtggtgg cggaagggcc
     9721 cgaggtggaa gacgactggc ataattttac ggcgctgaat tttcagcagg atcatccggc
     9781 ccgcgatatg caggatacct tttttatcga acgaaatcct gatatcgttc tgcggaccca
     9841 tacctcttcc gttcagatca gggctatgga aaaccgtaaa ccaccgcttc gggaaatata
     9901 cccggggcgc gttttccgca atgaagccat ctcggcccgc gcccactgca tctttcacca
     9961 ggtagaaggg ctgtatgtcg atcacgatgt gtcgtttgcc gatcttaaac aaacccttta
    10021 ctactttgcc catgagatgt tcggtgaagg gacagaggtt cgtttccggc ccagctactt
    10081 cccgttcacc gagccttcgg ccgaaatgga tgtcacctgc tttgtttgtg gaggcaaggg
    10141 ctgcccgatc tgcaaatact ccggctgggt ggagatcctg ggttgcggaa tggtcgatcc
    10201 caatgtcctt gagaattgtg gcatcgacag ccgggaatac accggcttcg ccttcggaat
    10261 ggggatagaa cggatcacga tattgaaata ccgaatccag gatattcgcc tgttcttcga
    10321 gaatgatgtg cgtttcctgc ggcagtttgc cggagtggtc tgatcactat tcctctatct
    10381 ttccagctgc atgatctcac cgatcagttt cggaatatcg gtgttatcga tataccctga
    10441 gaacttctca gcgccttgcc cgatggcata taccggcacg ctgacgaaag tgtgtgaacc
    10501 gcttccccat gaaattcctg ctttctcgtc gcgcaaaccg atgaccgact cgatgaattt
    10561 atcttcggga atataggttc cctgctccgg gccggcagca tacacacttt caagaaatct
    10621 ctctttcaaa tgggccagtt cgctgtcggt aagcagcgta ccattcgccc ggctgtgcaa
    10681 cccggtctca ttttccacca ccttcatcat ccgtaaaaaa tctgcatccg gatcacccga
    10741 tttgttgacc ctgaactgcg aaacgatttt attcatttcg tcagtggacg acaactggta
    10801 tttgagaagt tccaggttgg atgaatattt cattttccgg ttaccgaggg ccagaccacc
    10861 cgtttcgtgg tcggccgtca caatgatcag cgtttcctcg gggtgctttt cgtaaaaacg
    10921 gacagcgctt tcaatggccc ggctgaaaat gatgatctcc tggattaccg tggaggcatc
    10981 attggcatga caggcccagt cgatctttcc tccttccacc atcatgaaaa atccttcatc
    11041 accgctaaga agatcgatcg cttttgcggt ataatcggcc agcgtgatat ctccgggatc
    11101 catatcgata taatacggca tcgaagcttc gccggcaatc cgcggcgaca ggatcagggt
    11161 tttccccgtt cccggagcca gtttctcaaa ttcttcccgc gtatcagcta cattaaaccc
    11221 gttttccgct gccaggctga ccaggttgac ggtttgaccc ccgttcgtgc tgtcgggttg
    11281 aacaaaaccg ccgccggcaa acaaatcgaa acggctctgc gtcagttcgc ggccgatatc
    11341 gaaatagaga tcacggtcgg gctgatgggc ataaaaaacg gccggcgtag catgatcgat
    11401 cgacacactg gtgagaatcc ccactttgta gcctttgttc ttcgctttgg cagcaacaga
    11461 tgtcaggttc atggtcgcct ccggatccat ggaaatccgg ttaatattgg ttttattgcc
    11521 ggtggccaaa gctgttcctg ctgccgccga acaggtgatg agccggttat gagcatacgt
    11581 attgcaaaat ccggcctcgg gaaaccgcgt aaacgaaaga tcatcaaggc cgatcctgcc
    11641 atccagcgca gccaggtaag cctgtgccag gttgacctgg ccctgcccca tcccgtcgcc
    11701 gatgaacaaa aagacatatt tcgccctcgg ttcgcccgat gagggatcat tgccggctaa
    11761 ggcagtaatc tgaaaaaata tcgtataaat gatagcgaag acaaaaacgc tgctaaaaac
    11821 tcttcttttc ataaaactga tttcatttta cgttgtaaaa ataaacctta accgcttccc
    11881 ttttatgccc tttggtgctt tagtgtttta gtggcccaca tacggaaaac acctgtgtat
    11941 accctaacct ttaacaattg aacaatcgaa caatagaaca atcgatacct caattgattg
    12001 tacctttgca gccatgcagc aaaaactgaa ccctcaggat attgagatta tggccccggt
    12061 ggggagctat gaatcgcttt cggccgccat acaggggaaa gccgatgcgg tttacttcgg
    12121 catcgggcat ctgaacatgc gctcgcggtc gacacagaat ttctccctgg aggatctggc
    12181 agagattgct gcgatttgcc gcgaaaacca cctccgctct tacattaccc tgaataccga
    12241 aattttcgac gaagagattt ccctgatgcg ggcgatcgtg gatgaagcga aaaaagcggg
    12301 gatcacagcg ataatcgcct ccgaccaggc tgtgatccag tatgcccgga gccgggaggt
    12361 agaggtgcac atgtcgaccc aggccaacat caccaatacc gaagccgtaa agtattacgc
    12421 gcagtttgcc gatgtgatgg ttacagcgcg ggaactgaac ctggatcagg tgaagagaat
    12481 tactgagaac attgaaggag agcggattat aggtccttcg gggaagctgg tgcagattga
    12541 ggtgtttgcc catggcgctt tatgcatggc cgtttcgggc aaatgttacc tgagtctcga
    12601 caacatgaat tcctcggcca accgcggcgc ctgcctgcag ctctgccgcc ggccttaccg
    12661 ggtcttcgac cgcgacggcg aagtggagct ggaggttgat aaccagtaca tcatgtcgcc
    12721 caaagacctg aaaacgattg attttattga taagatcctg gatgcgggag tgagagtgct
    12781 gaagattgaa ggccgtggcc gctctcccga atatgtgaaa accgtcaccc gatgctaccg
    12841 cgaagccgtc aatgcccact gcgacggtac attcaatcct gaaaacctgg aacgctggaa
    12901 ccatgaactt ggaaccgttt ataatcgtgg cttctgggac ggttactacc tggggcggac
    12961 agtgggcgag tgggccgccg gctatggctc gcaggctaca aaaaagaaga tctatgtggg
    13021 gaaggtcatg aattattatg ccaggaccgg gatcgcggag gtgaagatcg aggcgcaccc
    13081 gttgcgcaaa ggcgatcatt tccttgttct cgggccaacg accggcgtgg ccgaagggat
    13141 taccccggat atttggctgg aagagaagat cgtgggcgaa gtgaaccgcg gcgacctgtg
    13201 cacttttcct ttcgatacgc ctctccggcc ttccgataaa ctttacaaat gggtcgacag
    13261 cagggaagtg gaagggcaga ttgattaaaa gcgtgattaa aaaggaactg ccccggatgg
    13321 gaaccggggc agtatacatt tttgccaaac ctaagggaga gaatgttagt cttctaacga
    13381 ctattaatcg gatttttgtg ataaggtaat ttattttaac gattttttta ttgatgatga
    13441 aatactttaa tcttcatact cattgcaatt actgtgatgg cgccggtttc ccggaggaat
    13501 atgttaatgc ggctattaag aaacaatttc ataccctggg attcagcagt catgcgccgg
    13561 ttccatttaa aaatactttt gctataagag atgagcagga acttcagaaa tattgtgatc
    13621 aaatcattca tcttaaagaa atttataaag ataaaattga tatttacctg ggattggaac
    13681 tcgattatat tgaagggatc agcggggatt ttggtaagat aaaagagaaa tacggtctcg
    13741 attacacgat tggatcggtc cacctggtaa gaaatggaga tgacgaaaac ttatggttca
    13801 tcgacggccc gagggtggag tcgtatgatg aagggttgaa aagtgttttt aaaggtgatg
    13861 taaaacaggc agtgacaacc tattatgagc aggttaaaaa gatggtcgtc acacagaagc
    13921 ccgatatcct ggggcatttc gacaagataa agatgcataa caaggaccgg tattttaagg
    13981 aagatgaggc gtggtaccgc gaccgggtga tggatttgct ggatgcgata tcaacgacgg
    14041 gtgtgatccc cgaggtcaat acccggggca tttacaaaaa gcggagcagc gatctttatc
    14101 ccggggtatg gatactaaag gaaatgaaga agatgaatat cccggtaatt ctcagcaccg
    14161 atgcccatca tccagtcgag atcgacgggt attttgatga aacggtcgga attctgaagg
    14221 aaatcggcta caattcgttg gtctgctaca atgcaaatgg ctggcaggaa ttacccattc
    14281 aaatcgaata tcgcccgttc gcctgttcgt gatttcggct gatgattaag atccctcctt
    14341 catcgggatg acgtcatccc gagcgtagcg agggatccgc cgaccgccga cctgttcata
    14401 actttccccg aaaacatata ttactttttc ctatatttaa cgatcaattg ccagattaac
    14461 caactatgtc cctgacatat acatatcgtg aatattttta ttctctgaag tttttcttaa
    14521 tttgccgccc cgctagtctt ctttctttct ttcttacgaa aacaggactt tccagagatt
    14581 agtttcttct ctcaatttcg aaaattccca tcgaacatca gcaatcgaac atcggcaatc
    14641 gaatatccat ttctcacact cccatcctcc atgaggggtt tgttacccca aaagtgaaaa
    14701 agaattaacc agaaaactcc acctccaaaa aatagtatta attttaaatc tgagtcttat
    14761 gaagaatttg atgaaaactt tggtgttaat ttcggtttta ttcatagccg gaatgcaaaa
    14821 tataaatgct caactaagca ttaaagtaat attggatggt caatgtcctg atacgtgtac
    14881 ttctcatgaa atttgcacat ataaagttga atatcaactg tacaatattt gcgtcgaacc
    14941 accagaactt tgctgcactg cacctccgca atgggtggat tgttctacgg aagaaatata
    15001 ttttaattgt gatttctcat gtgatgatcc ttcaaatgaa aaatgttatg cattggttgg
    15061 taaagcaact aaatactgca atggacctgg cggaaatata gcaatatgtg taggaactgg
    15121 aattgcatat tacacatgtc atgaagtaat gacattaagt ccattacctg taacagttta
    15181 ttggtgataa ttaaaatgaa tatctgtccg gcaagatgaa acgccggaca gattcttcaa
    15241 atactttaat aagatgcgaa ttccatttac gattcttcaa atattgatca cagttaaatt
    15301 tattattgca caaaacgaaa ctaatatatg gtattttacg aaaggctgtg gcttagattt
    15361 caattctgga aatccggaag ttctttacga tggacaggct atacatggtt gggcaagttc
    15421 aacaatgtgc gactcttcag gaatgttttt attttgtgca gatgccgagt atatttattc
    15481 ggtttatggt ataatgcaaa acagtgacgg attggtcaac cctcgcattg caggtttagc
    15541 ttcagtcaaa tggcctgaac aagaattctt gtattttcta tttacagctg cagatcctaa
    15601 tatacccaat gcaggattct attattcttt ggttgatcta agtcaaggcc ttggaggagt
    15661 ggttattgaa aaaaatattc cgattccttc cggttgggat gtaagtgata gaattgctat
    15721 agtaaaaaag gagaaatcag acaatgtctg gatcatagtc agaaaaaatg aagaagatgc
    15781 aattgcagct tacctattag atgaaatcgg attccatgct gatcctgtta tttcaacaat
    15841 gccaaattta gaaatgacct ggcgaagtat atgggggtat cttaaaatct ccacaaataa
    15901 aagttttttg atttccacat atggcagtta tggctttgaa gtctgcagtt tcgatgctgc
    15961 aaacggagtg gctgttcacc tttttctagc agatagtccc agccctaatt cgccgtcttg
    16021 ttcaggggtt gagttttcac ctgattcaaa atacctttat gtaaattata cgggacaagc
    16081 ttcaagtgaa ttgtatcaat atgatatgca atacgtgttt gattcggctt catttaaagc
    16141 gtctgcttta aaaatagaca gctataattc atttgaattg caattggcaa gggacggaaa
    16201 aatttattgt tcaccaaatc aggttttgga tcaaacaaac tggtataata taagtgtcat
    16261 agagaagcct tggattaggg gtgagggttg ctcttttaca caaaacgtgg ctaatgtatt
    16321 tccaaatcat gttttgtggg gatttcccaa catcctcgtc gactacctcc tccgcttcga
    16381 atggactggt gagccatgcc aaggctaccc ggtccagttc aaaccgaact tcatccctac
    16441 tcctcagacc attcaatggg atttcgatga cggacccgga agcacctcat ggcagctttc
    16501 tccaacatac gcttttaaaa atcctggcgt tcatgaggta aaggtcgatg tctggtatcc
    16561 ttccgggaga tatgagcata catccagaga aatcgaaatc tcaccttcac ctcaacctga
    16621 tcttggcccc gacaccatga tctgtgatgg cgcttccatt acgctcaatg ccaattgcga
    16681 agccgatttc ttctcctggt caaccggcca gttcggcgtt tcatcgatta ctgtttcaga
    16741 tagcggtaac tattgggtga gaggtcggtt caacgagtcc ggctgcgaag gatatgatac
    16801 catccatgta ggctttcacc cacctacaat catcgacgaa accaatcttg tcatcatccc
    16861 cacaacctgc aacggtgcaa gcggatcaat aaccggactc attgctcttg gcactccgcc
    16921 cttcgccttc caatggctcg acctctccgg caacccctac gggacgaaca tagatgcatc
    16981 gggccttccc gccgggcagt accagcttac catcaccgac ggcaatggct gcgaagcggt
    17041 ttctgaggtt tatacgatcg aagatgccgg caatctgcag gttttggaag cagaattaac
    17101 ccaaccgcac tgcggaaggc cggatggaga aatcgttgtt catgcctact ctccttccgg
    17161 atcaatactt gaatattcaa tcaacgatgg agcggactat acgtcagata gtgttttcac
    17221 cggattatct gccggaactt atgtcgtccg aatcagggat gtgaatggtt gtgatggatt
    17281 ttatctttca aaccctgttt accttgctga tattccaggt ccgcaggttg ttgaagtgaa
    17341 tgttacagat gaaacagatt ttcttgggaa cggagcaatt gagatcgtag ctaacggctc
    17401 tacgccattg atttattatt cgattgatgg cggagcgacc tggcaggtga atgacggaaa
    17461 ttttgagaat ctggaatctg gaatttggaa tttggtaatt aaggatgaga atggttgtga
    17521 tactactttt actgttgaaa tacagaatat tatactgaca tggttgcatg ctgttactgc
    17581 cgtaggaggg cactgtcttg gcaacacggc gatggttccg gtgaatgtcg ataatttcaa
    17641 tgctgttgcc gattttcacc tgaagctggg ctacaacgca gataacctgg aatgtgaagg
    17701 ctttgcaaat gtccatccgg agcttgccga cagtcttagt gggtgggtcg actacctggc
    17761 gggcgacatt cacctggcct ggaattcacc agcttctgtt accttcaccg gaacagaaaa
    17821 agtcgcagat ctggtgttca ccacgaaaaa ccccggacag ggagaactgg catggtacac
    17881 aggaaacgcg gagagctatt tcgccaattc ttccggcaac ccgatcccgg cggagttcag
    17941 caccggcgag gtgactgtct acgagccccc ggagatcatc ctggctcaga gcaaaaccgt
    18001 c
//
LOCUS       DAOPNH010000027        17744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3079_length_17744_cov_6.522189, whole genome shotgun sequence.
ACCESSION   DAOPNH010000027 DAOPNH010000000
VERSION     DAOPNH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17744
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_17744_cov_6.522189"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1121)
                     /locus_tag="PKZ74_02625"
     CDS             complement(102..1121)
                     /locus_tag="PKZ74_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91676.1"
                     /translation="MNRIAVFLFCLACLWAVSCTSPPARDKVPGESHKADTTVTVIER
                     LNRQIASDSANPDHYYRRSLYYLDKKEINQALSDIGKAIGFNDKNSSYFVTLSDVYLA
                     MGRIPSCLEALKKAESLDPANRDALLKMAEVYLYLRDYKNVQDYTKKALEIDRNSPTA
                     YFIRGYAALEQGDTSLAVQNFQRTVDLDQNHYGASIQLGGLFAEKNPALAAGYFQSAI
                     RIDPNRPEGYYLLGFLYQNQENIDKAIAAYEKLIVIAPEYKEAYYNLGYVNLVYTNDF
                     EASVRYFSQAVLLDSKYTDAFFNRGYSYELMGDVVNARKDYLRALEITPNYENAINGL
                     NRLDK"
     gene            complement(1145..2161)
                     /gene="recA"
                     /locus_tag="PKZ74_02630"
     CDS             complement(1145..2161)
                     /gene="recA"
                     /locus_tag="PKZ74_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPM91677.1"
                     /translation="MGGENNVDQTRKEKMKALEMTLGNIEKQYGKGTIMKLGDTPAEN
                     IPFIPTGSIGLDAALGVGGFPRGRVVEIYGPESSGKTTLALHAIAEAQKLGGIAAFID
                     AEHAFDRFYAQKLGIDIDNLLISQPDHGEQALEIVDNLIRSGAIDIIVIDSVAALTPK
                     SEIEGEMGDSKMGLQARLMSQALRKLTANIGRTGSCCIFINQLREKIGIMFGNPETTT
                     GGNALKFYASVRIDIRKINQIKEGEENTGNRVKVKVVKNKVAPPFKKAEFDIIYGQGI
                     SRIGEIIDMGSDFGIIKKSGSWFSYGETKLGQGRDAVKKLLTDNPELGEELEAKIREK
                     LIQS"
     gene            complement(2253..2711)
                     /gene="bcp"
                     /locus_tag="PKZ74_02635"
     CDS             complement(2253..2711)
                     /gene="bcp"
                     /locus_tag="PKZ74_02635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPM91678.1"
                     /translation="MNVKIGDKAPSISALDQNSNAFTLDSLRGSKVILYFYPKDDTPG
                     CTAEACNLRDNYSDLLNRGFKIVGVSTDSVKSHQKFSEKYSLPFPLLADTDKKIVNDY
                     GVWGKKKFMGREYDGTHRVTFVLSEEGTVLKIFDKVQTKEHAQQILMEIN"
     gene            complement(2802..3458)
                     /gene="nth"
                     /locus_tag="PKZ74_02640"
     CDS             complement(2802..3458)
                     /gene="nth"
                     /locus_tag="PKZ74_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPM91679.1"
                     /translation="MKKTERFKAFMAYFEENMPVAETELLYSNPYELLVSVILSAQCT
                     DKRVNMITPSFFRRFPDVGSLASAEKEEVFELVKSCSYPNNKSLHLLNMARMVMEKFG
                     GVIPDNTEDLQKLPGVGRKTAHVMVSVIYNQPKMAVDTHVFRVAARIGLTTGAKNPLQ
                     TELQLVKHIPEEKIPLAHHWLILHGRYVCRARNPACDECGMKPYCRFYEKLSKRQNLT
                     "
     gene            complement(3691..3885)
                     /locus_tag="PKZ74_02645"
     CDS             complement(3691..3885)
                     /locus_tag="PKZ74_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91680.1"
                     /translation="MDKTFTPLDIQRYLLEIRRIEFDSDKKKADSGPAALTIRNILNY
                     SRSLNIYKTSIMGNIFHVSN"
     gene            complement(3969..4556)
                     /locus_tag="PKZ74_02650"
     CDS             complement(3969..4556)
                     /locus_tag="PKZ74_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM91681.1"
                     /translation="MKAAIISDQELIGKYLNGNEESLELLINRHKRKVFAYIIMVVKD
                     DQLADDIFQDTFIKVINTIRSGSYKEEGKFIQWVMRIAHNLIIDHYRRAKRLPVVNNS
                     SDDFDIFNLVRITDPSVEEQIITSQIHEDVRNLIELLPNEQKEVLMMRHYSDMSFKEI
                     AEATDVSINTALGRMRYALINLRKIVKEKNIILTV"
     gene            4756..7581
                     /gene="uvrA"
                     /locus_tag="PKZ74_02655"
     CDS             4756..7581
                     /gene="uvrA"
                     /locus_tag="PKZ74_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPM91682.1"
                     /translation="MSGADFSQLDPKKFILVKGASVNNLKNIDVAIPRNRMVVITGLS
                     GSGKSSLAFDTLYADGQRRYVESLSAYARQFLGRMSKPRVEYIKGISPAIAIEQKVNT
                     RNPRSTVATSTEIYDYIKLLFARTGKTYSPVSGKLVKRHTVTDVTDFIMAQEEERRVT
                     ITCPLIRKADRDMKTELTLLLQQGFNRLRINGDFIRIEDVIHSGIPGKEKDIEILIDR
                     FSVNRSDEDLAGRVADSVQTAFYEGHGICTVRVEVNGQETLIEFSNRFERDGIVFEEP
                     SVNLFSFNNPYGACKRCEGFGSIIGIDEDLVVPNKGLSVYEDAIACWKGEKMGEWKEQ
                     LVMHAYKFDFPIHKPFYQLTDEQRLLLWTGNQYFQGLNEFFRFVEEQAYKIQYRVMLS
                     RYRGKTVCPECKGTRLRKDANYVKVGGKSVSEILLLPVSQALAFFKQVNLSDHDQKVS
                     QRLLTEIKTRLQFLEDVGLGYLSLNRQSSTLSGGESQRINLATSLGSSLVGSMYILDE
                     PSIGLHPRDTHRLINVLMNLRNLGNSVIVVEHDEEIIRASDYLIDIGPLAGSQGGEVV
                     FAGTFGQMQEDNKSLTSKYISGEMTIPVPKSRKPWKEYIEIMGARENNLKGIDVKFPL
                     NVLTVITGVSGSGKSSLVRNILYPALKKIYGGSSEKTGNYGRLEGDYNRIGQVEFVDQ
                     NPIGRSSRSNPATYLKAWDDIRNLYSDLQLAKVRGYKPGYFSFNIPGGRCEECEGEGV
                     VKIEMQFMADLSLTCESCEGKRFKDEVLDVRFQDRNIVDILEMTVEQAIAFFSSSQTH
                     ISTCRRIVEKLQPLSDVGLGYLQLGQSSSTLSGGEAQRIKLAYFLTKGGTSSPTLFIF
                     DEPTTGLHFHDISKLLDAFEALISKRHSVIVIEHNPEVIKCADWLIDLGPEGGDAGGH
                     VVFAGVPEGLVNVERSFTGKYLKEKIK"
     gene            complement(7645..8385)
                     /gene="rlmB"
                     /locus_tag="PKZ74_02660"
     CDS             complement(7645..8385)
                     /gene="rlmB"
                     /locus_tag="PKZ74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPM91683.1"
                     /translation="MEKENFIYGLHPVTEAIKSGKEIDRILFKKGLQGSFSAELLQLA
                     KTYQIPFQFVPQEKLDRITRKNHQGVIAMISQISYGSLENLVQMVFEQGKTPLFLILD
                     GITDVRNFGAISRTAECAGVDGIIIPMQGSAQINEEAVRTSAGALMRIPVCREKSLKS
                     TAAFLRESGITLFAATEHGKEPYFSGDYSGPAAIIMGSEETGISPELLRICDKMVQIP
                     MQGEISSLNVSVAAGVLLFEVVRQRMGS"
     gene            complement(8413..9675)
                     /locus_tag="PKZ74_02665"
     CDS             complement(8413..9675)
                     /locus_tag="PKZ74_02665"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPM91684.1"
                     /translation="MKSNLVHRLLWLLFIGMGVLTGCGTTGKVSLQNLVYLYHPDKQF
                     ATLNNCVYHTSDSTSTLFVEVLFNDLVYLKDPFTGLYACNYRLSYNLKEGYEKKEVLH
                     SSAVISGDSLNYGKSAKKVHSFELKTFYPEKYLLEITLYDLNRSGGTTVYREVDKSSR
                     QTRQNYLLLDINNELIWKDQRVEGEPFRIVSNRNDLNFLIVNRYVRDFPVARPPYTEE
                     NEPSFDYKPDSLYLQPVFNGQTDWIRLAGPGFYHFRQDTISRDGVTIFLYPEGFPEVT
                     TAGQLRYPLRYITTRKEYDTLTESENVKAAVDAFWLKTARTPDRAQLLFKKYYSNVED
                     ANRWFTSYQEGWKTDRGLIYIIFGKPGYVYRGEGFEEWIYGEPQNRNSLTFTFVKVIN
                     PFTDNDYMLLRSPTLKEPWFVTVQSWRR"
     gene            complement(9665..10987)
                     /gene="rmuC"
                     /locus_tag="PKZ74_02670"
     CDS             complement(9665..10987)
                     /gene="rmuC"
                     /locus_tag="PKZ74_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPM91685.1"
                     /translation="MIILYSGIALIIGVLITWLIGRTVSGKKVAFLKEQLAESDKENG
                     ILNERLMQARSEILKLEKSLEDERSAKSELVAGHARQAGEIDNMREKLTTQKSELEEL
                     QKKFATEFENIANRLLRENSREFTHLNQKNIGDILVPLREKIEKFEKKVDDTYQKGLV
                     EQTQLKDAIENLHKLNVRISEEANNLTRALKSDSKKMGNWGELILDRILEQSGLEKGK
                     EYYTQYTDRSEEGTVLRPDVVIALPDKKHIIIDSKVSLVAYDRFINGSSDEEKEKWQK
                     AHLESVREHIRGLSEKNYTGALTLDTPDFVLLFMPLESAFSLAVQHDPELFGYAWQRR
                     IVIVSPTTLLATLKTVESIWKHEKQTQNAMEIARQGKNLYDKFVNFIEDMEKLGNQIN
                     SVQNTYQDAHKKLTSGKGNLVRQAQNLISLGVKTEKSLSDKILPEDEE"
     gene            11077..11892
                     /locus_tag="PKZ74_02675"
     CDS             11077..11892
                     /locus_tag="PKZ74_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM91686.1"
                     /translation="MKTPALFLAYLMLISFSGAINIYGQSSSPVKPFGEPEFIQDFLC
                     SEVIYPETELSEGIEGKVIISFTVEPDGQVSQFGLKENISPGLDAEAVRLFGMLLWEP
                     ATEFGNPVRSVGEFPVDFNIKKYKKHCKARGYERTELPFQPVDSSNIVYETGQTDRKP
                     YPLFSEPGMSFNRFISQNISYPETAYRQGISGTVTLKFVIETSGRASNIKIINPVGGG
                     CSQEAIRLLKLLRWMPGIKGQGAVRTFMKIDIGFKLPENSEMNMFENSQMNPN"
     gene            complement(11893..12378)
                     /locus_tag="PKZ74_02680"
     CDS             complement(11893..12378)
                     /locus_tag="PKZ74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPM91687.1"
                     /translation="MIQRIQSLFLILAALASILLFFFPLAGIYASHATYKFYIYELQN
                     QVPLEPGEQPIFTFMTTLPLLVLNIGVIILTVITIFLYKNRITQMKLVRFAVLLEIVM
                     IALILFIYASIIEKNLFVSPDYLSEAGIYFPLVALVFLILANRSILKDEKLVRSVERL
                     R"
     gene            12485..13483
                     /locus_tag="PKZ74_02685"
     CDS             12485..13483
                     /locus_tag="PKZ74_02685"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM91688.1"
                     /translation="MYPKLSDLINDLLGTNLHLPFQTYGFFVAMGFVAGGLILFLELT
                     RKEKEKIIFAQPRKKKKGKPPVLETVMVRPRELTTNIILVAAVSGVIGAKIFDVIEHL
                     DRLFEDPAGTLFSFSGLAFYGGLITAAFTVSWYVERHKIPWAVNADAVAPGLMLAYGV
                     GRIGCQLSGDGCWGIVNPEPKPEWLSFLPDWMWAFNYPHNVINEGVLIPGCSRANCHV
                     LPEAVFPTPFYETIMALILFGILWSIRKKFSIPGYMFSTYLILNGIERLIIEQFRVNR
                     RYDLLGMEVTQATVIAVGLILAGIAGSFLFRRFHNRKVSILNTKNTRDSTKGHKRS"
     gene            13540..14343
                     /locus_tag="PKZ74_02690"
     CDS             13540..14343
                     /locus_tag="PKZ74_02690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPM91689.1"
                     /translation="MNFERLVREVVELCRNDGQYIRQQLELLRAKDIELKDDHNFVTY
                     VDRNSEAMLVENLSKLTPGCGFIAEENDYGSSGAGLKWVIDPLDGTTNFVHGIPLFAI
                     SVGLMEGDRVVAGVVHEVNRGESFYSWGDGRAYLNGKEIRVTEALTLKNSLLVTGFPY
                     IRDHILDEYMNLFKEYIRRSQDLRRLGSAATDLAYVAAGRFEGFYEISLNSWDVAAGS
                     FLVEQAGGRVTDFQGGDDFIFGRSLIASNGFVHDEMLAVAGEFFNIKAK"
     gene            14360..15745
                     /locus_tag="PKZ74_02695"
     CDS             14360..15745
                     /locus_tag="PKZ74_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPM91690.1"
                     /translation="MRYIGFNYQAFVAFLIILTGIHFISVAQDDNDQKNSPVQVYKFD
                     IKKEIAPPVWHSTKKALEEAVAQNADVILIEMSTYGGMLESADSIRTKILNSPIPVIC
                     FIHHNAASAGALIAIACDSIYMSTGSSIGAATVVDQAGQVVPDKYQSYMRAMMRATAE
                     ATGRDPNIAQAMVDPRIEIEGVIDSGSVLTFTASEAMKFGYCNGIAETVDEVIGKAGI
                     KDYEIIEQKLTAIDKIIGFLISPVISGILIMVIVGGIYFELQTPGIGFPSIAAVTAAL
                     LYFAPLYLEGLAAHWEILIFIVGIILVAIEIFAIPGFGIAGISGIVLIITGLTLSMIG
                     NIGLDFRPVNFNNFIVSFFIVIISIFLSIIGSYFLTKSMFSRNRIFGSLALETVESAE
                     EGYTTSDSSYREMVGKTGTAHSILRPVGKVNIGGEIYDATALTGYIDKGEKIEVIRYE
                     TTQLFVRKIEN"
     gene            15982..16824
                     /locus_tag="PKZ74_02700"
     CDS             15982..16824
                     /locus_tag="PKZ74_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPM91691.1"
                     /translation="MILIADAGATKIQWVVIHDGKAGNPFETNGFNPYFMAPKILEDA
                     VEKDLVPFLSPPHIKQIYYYGAGCSTVQKCGIVDDVLRDIFENADIDIHHDLLGAARS
                     LFGKVEGIACILGTGSNSCLYDGKTILENVTSLGYLFGDEGSGAYMGKLFLTEYLRKR
                     MPQHLTEEFDRKFGFTVENILDSVYNKPHPNQFLSSFSKFIGEHKEDEFIKTIARQNF
                     RDFFREQVTRYTDYKQKPLGVIGSVGFHYQEIFREVASEHGLAVSKLVQSPLQGLVEY
                     HGGK"
     gene            complement(16783..17694)
                     /gene="miaA"
                     /locus_tag="PKZ74_02705"
     CDS             complement(16783..17694)
                     /gene="miaA"
                     /locus_tag="PKZ74_02705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPM91692.1"
                     /translation="MSSPVAKTLLIILGPTASGKTELAIRAAQWLDTEIISADSRQFY
                     RELSIGTAKPSPEQLSAVRHHFIGHLSVTEDYNIARFENDAIELLEVLFEKHDVVVMC
                     GGSGLYIDAVCHGIDEQPEHNPQIRKQLTEKYREKGIGYLQEQLQKLDPGYFNTVDVS
                     NPQRLLRAVEVCLITGKPYSGYRKGELKDRSFRIVKIGLEISREKLTERINRRTDEMI
                     GAGLVDEVKGLFSYRHLNALNTVGYKEIFCYLDGEWSLEEATEKIKINTRRYAKRQMT
                     WFRKDKTVTWIDPELFSPVVLHEALQR"
BASE COUNT         4510 a         4348 c         4229 g         4657 t
ORIGIN      
        1 ccataagaaa agaatcggga agatggaaat ggccggcgtt tgttgccgtt tataccacgg
       61 cgctggcctg gctggcggca ttcgccgttt accagatcgg gctacttatc caaccggttt
      121 aatccgttga tggcattctc ataattcggc gttatctcca gcgctcggag atagtctttc
      181 cgggcgttga caacatcgcc catcagttca taactgtaac ccctgttgaa aaaggcatcg
      241 gtatatttag aatcaaggag cacggcttgc gagaaatacc tcacggatgc ctcaaaatca
      301 ttcgtataca ccagattcac ataaccaagg ttataataag cttctttata ctccggggcg
      361 atcacgatga gcttttcata ggcagcaatg gctttatcga tattttcctg gttctggtaa
      421 agaaatccca gcaggtaata tccttccggc cggttaggat cgatccggat ggccgactgg
      481 aaatatcccg ccgccaatgc cggattcttt tcggcaaata aaccgcctaa ctggatactg
      541 gcgccataat ggttctggtc gaggtcgacc gtacgctgga aattctggac agcgagtgac
      601 gtatctccct gttccagcgc cgcatatccc cggataaaat aagctgtagg actgttccgg
      661 tcgatttcca gtgccttttt tgtgtagtcc tggacatttt tgtaatccct cagataaagg
      721 taaacttcag ccatcttcag caatgcatct ctgtttgccg gatcgagtga ttcagccttc
      781 ttcagggcct ccaggcagct gggtatgcgg cccatcgcca ggtaaacatc cgataaagtc
      841 acgaaatagg aggaattctt atcattgaac ccaatggctt tcccgatatc cgataaggcc
      901 tggttgatct cttttttgtc gagataataa agcgagcggc ggtaataatg atccgggttg
      961 gccgaatcgg atgcaatttg ccggttaaga cgttcaataa cggttaccgt tgtatcggct
     1021 ttatgggatt cacccggcac cttgtcgcgg gccgggggac tggtgcagga aaccgcccac
     1081 aggcaggcaa gacagaaaag gaatacagca atccggttca tttcaaaggg ataaataagc
     1141 aggttcagga ctgaatgagc ttttcgcgga tttttgcctc cagttcttca cccagttcgg
     1201 gattatccgt aagcaacttt tttaccgcat cccggccctg tccgagtttt gtttctccgt
     1261 atgagaacca ggaaccgctc tttttaatga tcccgaaatc ggagcccata tcgatgatct
     1321 cgccgatacg gctgatcccc tggccgtata ttatatcgaa ttcagccttt ttgaacggag
     1381 gggccacttt attcttgact actttcacct tgaccctgtt cccggtattc tcttccccct
     1441 ccttgatctg gttgatcttg cggatgtcaa tcctgaccga agcatagaat ttcagggcat
     1501 ttccaccggt tgttgtttcg gggttaccga acatgatacc gatcttttcc ctgagctggt
     1561 tgataaaaat acagcaagac ccggtcctgc cgatattggc agtcagtttc cggagggcct
     1621 gcgacatcag cctggcctgc aatcccatct tggagtcgcc catctcgcct tcgatctcac
     1681 ttttgggggt tagcgccgca acggagtcaa taacaataat atcgatcgct cccgaccgga
     1741 tcaggttatc cactatctcc agggcctgtt ccccgtgatc cggctgggag atcagcaggt
     1801 tgtcgatatc gatgcccagc ttctgcgcgt agaaacggtc gaaagcatgc tcggcatcga
     1861 tgaatgctgc aatccccccc agtttttgtg cttcggcgat agcatgcagc gccagtgtgg
     1921 tttttccgct ggattccggg ccgtaaattt caaccaccct tcccctgggg aatccgccga
     1981 ctcccaatgc cgcatcgagc ccgatactgc ccgttggaat aaaaggaata ttctcagcag
     2041 gtgtatcacc caatttcatg atagtccctt ttccatactg tttctcaatg ttgcccaggg
     2101 tcatttccag ggctttcatc ttttctttcc ttgtctggtc gacgttgttc tctccgccca
     2161 taatattaag atttgtggat ttgtagattt gtggatttga agatttttga ttttgatttt
     2221 gattttgatt tgtctgtttg gatgttacta ttttaattga tttccatcag gatctgctga
     2281 gcatgttctt ttgtctggac tttatcaaat atcttcaaaa cagtcccttc ttccgaaaga
     2341 acgaaggtaa cccggtgcgt tccgtcatat tcccttccca taaacttctt ctttccccat
     2401 acaccataat cgttgactat ttttttatcg gtatcggcca gtaaagggaa tggcagggaa
     2461 tatttttcag aaaacttctg gtgcgatttc acactgtcgg tgctcacgcc aacaattttg
     2521 aaaccacggt tcagcaagtc cgaataatta tcacgcaaat tacaggcttc ggctgtacat
     2581 cccggcgtat catctttcgg ataaaaatag aggatcactt tgctgccccg gagactatcc
     2641 agggtaaagg cattgctatt ctggtccaga gcgctgatcg acggggcttt atccccaatt
     2701 tttacattca taaaagttaa ttttaataat gacaaaaata ttgattttaa ttgttttatg
     2761 attaacctaa aggcattagt tatcaaggtt tacgaacaac ttcacgtcaa attctgccgc
     2821 ttcgacaatt tttcataaaa ccggcagtaa ggtttcatcc cacattcatc acatgcagga
     2881 ttcctggcac ggcatacata tcttccgtgc agaatcagcc aatggtgggc cagcggtatt
     2941 ttttcctccg gaatgtgctt taccagctgc aattccgtct gcagcgggtt tttagcaccg
     3001 gtcgtcaggc caatccgtgc agcaaccctg aaaacgtgcg tatcaactgc catcttcggc
     3061 tggttataaa taaccgaaac cataacatga gctgttttgc gccccactcc gggaagtttc
     3121 tgaagatctt ccgtgttatc agggataacc cccccgaact tttccataac cattcttgcc
     3181 atatttaaca gatgcaacga tttattgttg ggataggaac aactttttac aagttcgaac
     3241 acctcctcct tttctgccga tgccagtgac cccacatcgg gaaaccgcct gaaaaacgac
     3301 ggggtaatca tattgaccct tttgtcggta cattgtgccg ataaaatgac agataccagc
     3361 aattcatatg gatttgaata cagcagctcg gtttcggcaa ccggcatgtt ttcttcaaaa
     3421 taggccataa aagctttaaa ccgttccgtt tttttcatct tctataatga ttaatcggaa
     3481 aaacaggtaa aaaatacccg gtggttatga aataaatttt tcaacatgaa aatatttggt
     3541 atatcaatct gaaaagcaat gagttatgaa aaatgattta atatctgatt ttgacagata
     3601 ttcagaagaa aaaaccatta tgtaccgacg gattcacccg tcggactata atctgaacaa
     3661 acagaatatg ttttaaggac tactcctctc tcaattcgaa acatgaaaga tgttgcccat
     3721 tattgaggtc ttatagatat ttaacgaccg ggaatagttc aggatattcc tgatagtcaa
     3781 cgctgccggg cctgaatcgg ctttcttttt atccgagtca aattctattc gcctgatctc
     3841 cagtaaatag cgttgtatgt caagtggggt aaaggtttta tccataggct gatttagttg
     3901 ttaaggaact ataacaggca aacggataaa accggtattt atttttggaa tacgaaaaaa
     3961 actataagtt aaactgtcag gataatattc ttctctttaa caattttcct gaggttgatc
     4021 agcgcataac gcatccggcc gagggctgta ttgatactga catcggtcgc ttcggcgatc
     4081 tctttaaaac tcatgtcgct gtaatggcgc atcatcagca cttccttttg ctcattcgga
     4141 agcagttcaa tgagatttct gacatcttca tggatctggc tggtaatgat ctgctcttcc
     4201 acggaaggat cggttatcct gaccaggttg aaaatatcaa aatcgtcgct ggaattgtta
     4261 accaccggca atcgctttgc ccgccggtaa tggtcgatga tcaggttatg ggcaatccgc
     4321 atgacccatt ggatgaactt gccctcttcc ttgtatgagc ctgaacgaat cgtgttgatc
     4381 acctttatga aagtatcctg gaaaatatca tcagcaagct ggtcatcttt aacgaccatg
     4441 atgatatatg caaaaacttt gcgtttgtga cggttgatga gtaattccag actctcctca
     4501 ttaccgttta aatatttacc aattagctcc tgatcgctaa tgatggcagc tttcatagtg
     4561 cctccttttt taggtgaata ctgtaaaaaa gggtaaatat ttaatcgata attttcctcc
     4621 tgtaatttaa aggtcagatg aaatatttca atttactttc gatgaaagaa gtgcaaatat
     4681 agtcaataaa aaaataaatg caagtgaata ttcatatttt tgctcttttt ttcccaacag
     4741 attaacttgt tttgcatgtc aggcgcagat ttcagccagc tcgacccgaa gaaattcatc
     4801 ctcgtcaaag gcgcatcggt caacaattta aagaacatcg acgtagctat tccccggaac
     4861 aggatggtag tgatcaccgg gttatcgggt tcgggaaagt cgtcgctggc attcgacacc
     4921 ctgtatgccg acgggcagag gcggtatgtg gaaagcctga gcgcctatgc acggcagttc
     4981 ctgggaagga tgagcaaacc ccgggtcgaa tatataaaag gtatatcgcc ggctatcgct
     5041 atcgaacaga aggtcaatac gcgcaatccg cgttcgacag tcgctacatc gaccgaaatc
     5101 tacgattata tcaaattgct gttcgcccgc accggtaaga cctactcgcc tgtctcgggc
     5161 aagctggtca agaggcatac ggtaacggat gtcaccgatt ttatcatggc gcaggaagaa
     5221 gagcgccggg taaccatcac ctgccccctg atcaggaaag ccgaccggga tatgaaaacc
     5281 gaactcactt tgctcctgca gcaaggtttc aaccgcctga gaatcaacgg tgatttcatc
     5341 cggattgaag atgtgatcca ttccggcatt cctggcaagg agaaggatat tgagatcctc
     5401 atcgaccggt tttctgttaa ccggtccgac gaagatctgg ccggccgggt ggctgattct
     5461 gttcaaacgg cattttatga aggccatggc atatgtactg tcagggttga ggttaatggc
     5521 caggagaccc ttattgaatt ttccaaccgg tttgagcggg acgggatcgt atttgaagag
     5581 ccttccgtta acctgttcag cttcaataat ccttacggcg catgcaaacg ctgcgaaggt
     5641 ttcggcagca tcatcggcat cgatgaagat cttgtcgtac caaacaaagg attgtctgta
     5701 tatgaggatg ccatagcctg ctggaaaggg gaaaagatgg gcgaatggaa agaacagctg
     5761 gtgatgcatg cctataaatt cgacttcccc atccacaaac ccttttatca gctgaccgat
     5821 gaacagcgcc tcctgctctg gaccggaaat cagtatttcc aggggctgaa cgagtttttc
     5881 aggtttgtgg aagaacaggc ttataaaatc cagtaccggg tgatgctgtc gaggtaccgc
     5941 ggcaagaccg tttgccccga atgcaaaggg acgcggctcc ggaaagatgc gaattatgta
     6001 aaagtcggcg gaaaatcggt gagcgagatc ctgcttttgc ctgtttccca ggcgctggca
     6061 tttttcaaac aggtaaattt atcggatcat gatcagaaag tttcccagcg cttgctgaca
     6121 gagataaaaa cccgcctgca atttctcgaa gatgtaggcc tgggctatct atcgctgaac
     6181 cgccagtcat caacgctatc gggaggagaa tcccagcgga tcaacctggc cacttcgctg
     6241 ggcagcagcc tggtcggatc gatgtatatc ctcgacgagc ccagtatcgg actgcatccc
     6301 cgcgatacgc accgactgat caatgtgctg atgaacctga gaaacctggg caattcggtg
     6361 atcgttgttg aacacgacga agaaatcatc agagcttctg attatctgat cgatatcggg
     6421 ccgctggccg gcagccaggg cggtgaagtc gtgtttgcag ggactttcgg acaaatgcag
     6481 gaagacaata aaagcctcac ctctaagtat atttcaggag aaatgaccat tcccgttccg
     6541 aaaagcagga agccgtggaa ggaatatatt gaaatcatgg gcgcccgtga aaacaacctg
     6601 aaagggatcg atgtgaagtt cccattgaat gtactgacgg ttataaccgg ggtcagcgga
     6661 tcgggcaagt cgtcgctggt gcgtaatatc ctgtacccgg ccctgaaaaa gatctatggc
     6721 ggaagcagcg agaaaacagg gaactatgga cgactggaag gcgactacaa ccggatcgga
     6781 caggtggagt ttgtcgacca gaacccgatc ggccgctctt cacgttctaa tccggctaca
     6841 tatctgaaag cctgggacga tatccgtaat ttatattccg acctgcaatt ggcaaaagtc
     6901 aggggatata aaccgggtta tttttcattc aatattccgg gtggaagatg tgaagaatgt
     6961 gaaggcgaag gggtggtgaa gatcgagatg cagttcatgg ccgacctgag ccttacctgt
     7021 gaatcatgcg aaggaaaaag gttcaaggat gaggtcctgg atgtgcggtt ccaggataga
     7081 aacatcgtcg atatcctgga aatgactgtt gagcaggcca ttgcgttctt ttcatcttca
     7141 caaactcaca tttccacctg ccggcggatc gttgaaaagc ttcaaccgct aagcgatgtg
     7201 ggcctgggct atctccagct cggccaatcg tcgagcacct taagcggtgg cgaagcgcag
     7261 cgtatcaaac tggcctattt tctgaccaaa ggcggaactt catcccctac cctgttcatc
     7321 ttcgatgaac ccacaaccgg cctgcacttc cacgatatca gcaaactcct cgacgctttc
     7381 gaagccctca ttagcaaaag gcactcggtg atcgtgatcg agcataaccc ggaggtgatc
     7441 aaatgcgccg actggctgat cgacctgggc ccggagggag gcgatgccgg aggacacgtt
     7501 gtttttgccg gcgttcccga aggccttgtt aatgttgagc gttcgtttac cggaaagtac
     7561 ctgaaagaaa aaatcaaatg aattcagtcc acaatcttca aataagatcc aagattgtaa
     7621 actgtaaact gtaaaccgta aatcctacga tcccatcctt tgccttacca cctcgaacag
     7681 aaggacaccg gcggctacag agacattcaa tgatgatatc tcgccctgca tgggtatctg
     7741 taccatttta tcacaaatcc gcaataattc gggtgaaatg ccggtctctt ccgagcccat
     7801 gatgatcgca gccggaccgg aataatcgcc cgaaaaataa ggttctttac cgtgttcggt
     7861 ggcggcaaag agcgtgatcc cgctctccct gagaaaagca gcggttgatt tcaacgactt
     7921 ttcacggcat accggaatgc gcatcagtgc gccggcgctg gtcctgaccg cttcctcgtt
     7981 gatctgggcg ctgccctgca tcgggataat gatgccatcg acacctgcgc actcggccgt
     8041 gcggcttatg gcgccgaagt tgcgaacatc cgtaattccg tcgaggatca ggaacagagg
     8101 tgtttttccc tgctcgaaca ccatttgcac caggttttcc agtgagccat agctgatctg
     8161 cgagatcatg gcgatcactc cctggtgatt tttccgggta atgcgatcga gcttttcctg
     8221 cgggacgaac tggaaaggta tctggtacgt tttggcaagt tgcagcagtt cggcagaaaa
     8281 agatccctgc aacccttttt taaacaaaat ccggtcgatc tctttgccac ttttgatggc
     8341 ttccgtcacc gggtgcaaac cgtatataaa gttttctttt tccattctct tgcttagttt
     8401 agcggtcaag tatcatcgac gccagctctg aacagttaca aaccaaggct ctttcagcgt
     8461 cggggaacgc agtagcatgt aatcgttgtc agtaaaggga ttgataactt tcacaaaggt
     8521 aaatgtcagg gaattgcgat tctgcggctc accatagatc cattcttcaa atccttctcc
     8581 acggtaaaca tatccgggtt tcccgaagat gatatatata agccctcggt cggtcttcca
     8641 gccctcctgg tatgaagtaa accatcggtt cgcatcttcg acattcgaat aatatttttt
     8701 gaacaacaat tgcgcccggt cgggagtgcg ggccgttttc agccagaaag catcgacggc
     8761 ggctttgaca ttctccgatt cggtaagggt atcgtattcc ttgcgcgtag taatgtatcg
     8821 cagcggatat cgcagttgcc cggcagtcgt aacctccgga aaaccttcag gataaaggaa
     8881 aatggttacg ccgtcacggg aaatggtatc ctgccggaag tggtaaaatc ccgggccggc
     8941 aagcctgatc cagtcggtct gcccgttgaa caccggctga aggtaaaggc tgtcgggctt
     9001 gtaatcaaag gatggttcat tttcttcggt gtaaggaggc ctggctacag gaaagtcccg
     9061 gacataacgg ttgacaatga ggaaattgag gtcattccgg ttcgatacga tccggaacgg
     9121 ctctccttcc acccgttgat ccttccatat caactcatta ttgatatcca aaagaagata
     9181 attctgccgc gtctgccggg aacttttatc gacctcccgg taaacggttg tgcccccgct
     9241 ccggtttaaa tcgtataagg taatctcaag caggtacttt tcgggataaa aagttttcaa
     9301 ttcaaacgaa tggacctttt ttgcactctt tccgtagttc agcgaatcac cggatataac
     9361 agccgagcta tgcagaacct ccttcttttc atacccttcc ttcagattat agctgagcct
     9421 gtaattgcag gcataaagac cggtaaaagg gtctttcaga taaaccagat cattgaacaa
     9481 cacctcaaca aaaagggttg aagtggaatc ggacgtgtgg taaacgcaat tattgagtgt
     9541 ggcaaattgt ttatcaggat ggtagagata caccaggttt tgaaggctga cttttccggt
     9601 cgtgccgcag ccggtcagaa cccccattcc gatgaacagc agccaaagta accggtgcac
     9661 aagattactc ttcatcctcg ggaagtattt tatccgacag cgacttctca gttttgactc
     9721 ccaggctgat caggttctga gcctggcgca ccaggtttcc cttcccgctg gtcagctttt
     9781 tgtgcgcatc ctggtatgta ttctgaacgg aattgatctg gtttcccagt ttttccatat
     9841 cttcgataaa gttcacgaat ttatcgtaca ggtttttccc ctggcgcgct atttccatcg
     9901 cattctgcgt ctgcttttcg tgcttccaga tcgattcgac tgttttcagg gtcgccagca
     9961 aagttgtcgg gctaacgatg acgatcctcc gttgccaggc ataaccgaac agttccgggt
    10021 cgtgctggac tgccaggctg aaagccgatt cgagcggcat gaacagcagt acgaagtcgg
    10081 gtgtatccaa agtcaaagca cctgtgtaat tcttttcgct caatccccgg atatgttccc
    10141 tgaccgattc caggtgggcc ttctgccatt tttccttctc ctcgtcgctg cttccgttga
    10201 taaaccggtc ataagcgacc agtgatactt ttgagtcgat aatgatatgt ttcttatcgg
    10261 gcagtgcgat tacgacatcg ggtcgtaata cggtaccctc ttccgaccgg tcggtgtact
    10321 gggtataata ctccttgcct ttttccagcc ctgactgctc cagtatccgg tcgaggatca
    10381 gctcgcccca gttgcccatc ttcttggaat cggacttcag ggcccgcgtc aggttgttgg
    10441 cttcttcgct gatcctgaca ttgagcttgt ggaggttctc aatggcgtct ttaagctggg
    10501 tttgttctac cagtcctttc tggtaagtat cgtcgacctt tttctcaaat ttctcaattt
    10561 tctcccgcag tggtaccagg atatctccaa tattcttttg attcagatgg gtaaactccc
    10621 gggaattctc acggagaagc cggttggcaa tattctcgaa ctcggttgca aacttcttct
    10681 gcaactcttc caattcgctc ttctgggtgg tgagcttttc tctcatgttg tcaatctcac
    10741 cggcctggcg ggcatggccg gcaaccagct cagattttgc acttcgctca tcttcaaggc
    10801 ttttctccag tttcaggatc tcactgcgtg cctgcatcaa ccgttcgttc aaaatcccgt
    10861 tttctttatc cgattccgcg agctgctctt tcagaaaagc gacctttttg cccgaaacag
    10921 ttctcccgat cagccaggta attaacaccc cgattattaa tgcaattccc gaatataaaa
    10981 tgatcatgac cattttttcg ataaaaataa ccatttcgga aagtcattct tgcattacgg
    11041 tgcaaatttc cttacattta ccacaaacct ttggtaatga aaacaccggc attatttctg
    11101 gcgtatttga tgcttatatc cttttccggg gcgatcaata tttacggaca aagttccagt
    11161 cctgtaaagc cttttggtga acccgaattt atccaggatt ttctttgttc ggaagtcatt
    11221 tacccggaaa cggaactgtc ggaaggaata gagggaaaag taatcatatc gttcaccgta
    11281 gagccggatg gacaggtcag tcagttcggg ctaaaagaga atatttcgcc cgggctcgat
    11341 gccgaagcgg tcaggttgtt cggtatgctg ctctgggagc cggccaccga atttgggaat
    11401 cctgtcaggt cagtcggtga atttccggtt gacttcaata taaaaaaata caagaaacat
    11461 tgtaaagcca ggggatacga acgtacagaa ctccccttcc agccggtcga ttcgtcgaat
    11521 atcgtttatg aaaccggcca gaccgaccga aagccatatc cgttgttttc cgagccgggg
    11581 atgagcttta accggttcat ctcacaaaat ataagttacc ctgaaacggc ttacaggcaa
    11641 gggatctcag gcaccgttac cctaaagttt gttatagaaa ccagcggcag ggcctctaac
    11701 ataaagatta tcaatcccgt aggtggagga tgctcgcagg aggcaatacg actcttaaaa
    11761 ttgctacgct ggatgcccgg tattaaaggc cagggagcgg tcaggacttt catgaagata
    11821 gatatcggct ttaaactgcc ggagaattct gaaatgaata tgttcgagaa ctcccagatg
    11881 aacccgaact gatcagcgga ggcgctcgac cgaacggacc agcttctcat cctttaaaat
    11941 cgaccggttg gccaggatca ggaaaaccag cgccaccagc ggaaaataga ttcccgcttc
    12001 gctcaggtaa tcgggtgaaa caaacagatt tttctcgata atgctggcat agataaacag
    12061 gatcagcgcg atcatcacta tctcaagcaa cacggcgaac cggaccagtt tcatctgtgt
    12121 tatcctgttt ttgtaaagaa agatagtgat aaccgtaagg atgataaccc cgatattcag
    12181 caccagcaaa ggcaacgtgg tcatgaaggt aaatatcggc tgttctcccg gttccagcgg
    12241 aacctggttt tgcagttcat agatatagaa tttatacgtg gcatgactgg catatatgcc
    12301 ggccagcggg aaaaagaaca gcaaaatgga ggctaaggcc gccaggatca ggaacaatga
    12361 ttgaatacgt tgaatcataa tgcgatgctt acagataaaa attgaattca gtaaaaaccc
    12421 gaatgcaaag ataacttgtt aattttgcca tcaggccggc acgaaagaaa ttaatcctct
    12481 atttatgtat ccaaaactga gcgacctgat aaacgatctc ctgggaacca atctccacct
    12541 gcccttccag acctatgggt tctttgtggc gatgggtttt gtggcgggag gactgatcct
    12601 gttcctggag cttacacgca aagagaaaga gaagatcatt ttcgcacagc cccgcaagaa
    12661 aaagaaaggc aaaccgccgg ttctggaaac agtgatggta aggccccggg aactgacgac
    12721 aaacatcatc ctggtagcgg ctgtctcggg agttatcggc gccaaaatat tcgatgtgat
    12781 cgagcatctc gaccgtttat ttgaagatcc ggccggcacc ttgttttcct tcagcgggct
    12841 cgccttttac ggcggcctga tcaccgcggc attcacggtc tcctggtatg tcgaaaggca
    12901 caagatccca tgggctgtaa atgccgacgc tgttgcaccc gggctgatgc tggcttacgg
    12961 tgtcggacgg atcggatgcc agctttcggg cgacgggtgc tggggaatcg taaatcccga
    13021 acccaagccg gagtggctct ccttcctgcc cgactggatg tgggctttca actacccgca
    13081 caatgtgatc aacgaagggg tgctgatccc cggctgctcg cgggcaaact gccatgtttt
    13141 gcccgaggcc gtttttccga cgccattcta tgaaaccatc atggcactga tccttttcgg
    13201 catcctctgg agcatccgga aaaagttttc cataccgggg tacatgttct ccacttattt
    13261 gatcctgaat ggaatagagc ggcttatcat cgagcagttc cgggtcaaca ggcgttatga
    13321 tctgctggga atggaggtga cacaagcaac ggtcattgcc gtgggtttaa tcctggcggg
    13381 catagccgga tcctttttat tcagacgatt tcataataga aaagtaagta ttttaaacac
    13441 aaagaacaca agggatagca caaaggggca caaaagaagt tgaaacttct tgcgccttcg
    13501 ggaattacac agcttgactg ttcagaaaat atatcaacca tgaattttga aagactggtc
    13561 cgagaggttg ttgaactgtg ccggaacgac ggacaatata tccggcagca gcttgaactg
    13621 ctccgggcta aagatatcga gctgaaagac gatcacaatt ttgtgaccta tgttgaccgg
    13681 aattcggaag ccatgctggt ggagaatcta tcgaaattga cgccgggctg cggttttatt
    13741 gccgaagaga atgattacgg gagttccggc gctgggttga aatgggtaat cgatccgctc
    13801 gacggcacca ccaattttgt gcatgggatt ccgttatttg ccatcagtgt cggcctgatg
    13861 gaaggagacc gggtggttgc aggcgtggtg cacgaagtca accggggaga aagtttttat
    13921 tcctgggggg acgggcgggc ctatctgaac ggaaaggaga tcagggtaac agaagccctg
    13981 accttgaaga acagtcttct ggtcaccgga tttccatata tccgggatca tatcctggat
    14041 gagtacatga acctgttcaa ggagtatatc cgccggagcc aggatttgcg ccggctgggc
    14101 tcggcggcaa ccgacctggc ttatgtggca gcgggacgat tcgaagggtt ctatgagatc
    14161 agcctgaatt cctgggatgt ggccgccgga tcattcctgg tggaacaagc cggtggaaga
    14221 gtaaccgatt tccagggggg tgatgacttt atcttcggcc ggtcactgat tgccagtaac
    14281 ggtttcgtgc atgacgaaat gctggcggtg gcaggggaat tttttaacat aaaagcaaaa
    14341 taacactaaa taatagtaga tgagatatat aggattcaat taccaggcat tcgttgcatt
    14401 tttaattatc ctgaccggaa ttcactttat ttctgttgcc caggatgata atgatcagaa
    14461 gaacagccct gtacaggtgt ataaattcga catcaaaaaa gaaatcgcac cgccggtctg
    14521 gcattcgacg aaaaaagcgc tggaagaagc ggtggcgcaa aatgccgatg ttatcctgat
    14581 cgaaatgagc acctacggcg gtatgctgga gtcggccgac tccatccgta ccaaaatcct
    14641 caactcccct atcccggtga tctgctttat ccatcacaac gccgcatcgg ccggggcttt
    14701 gatcgctatc gcctgcgaca gcatctacat gtcgaccggc tcttccatcg gggcagctac
    14761 ggtggtcgac caggccggcc aggtggtgcc cgacaaatac cagtcgtaca tgagggccat
    14821 gatgcgtgcc accgccgaag ccaccggcag agacccgaat attgcccagg cgatggtcga
    14881 cccgagaata gaaatcgaag gggtgatcga ttcgggcagc gtcctcactt tcaccgcatc
    14941 ggaagcaatg aaattcggct actgcaacgg aatagccgaa actgtcgatg aagtcatagg
    15001 gaaagccggc ataaaagact acgaaattat cgagcagaag ctcaccgcca tcgataaaat
    15061 catcgggttc ctgatcagcc cggtcatcag cggaatcctg atcatggtca tcgtcggtgg
    15121 aatctatttc gaactccaga cgcccgggat cggtttccct tccatcgcag ccgtcaccgc
    15181 tgcactcctc tatttcgcgc ccctctacct cgaagggctg gccgcacact gggaaatcct
    15241 catctttatc gtcgggatca tcctggtggc catcgagatc tttgccatac ccggcttcgg
    15301 tattgccggg atttccggta tcgtactcat aatcaccggc ctgaccctca gcatgatcgg
    15361 gaatatcggc ctcgacttca ggcctgtcaa cttcaacaac tttatcgtct ccttcttcat
    15421 cgtgatcatc tcgatcttcc tttcgatcat cggttcctat ttcctgacca aatcgatgtt
    15481 ctcacgaaac cggatcttcg ggtcgctggc gctggaaacc gtggaaagcg ccgaggaagg
    15541 atacacgact tccgacagca gttataggga gatggtcgga aaaacgggca cggcccattc
    15601 gatcctgcga ccggtgggaa aagtgaatat cggcggcgaa atctacgacg ctaccgccct
    15661 gaccggctat atcgacaaag gggagaaaat cgaggtcatc aggtacgaaa ccacacaact
    15721 tttcgttaga aagattgaaa actgatagct gaaaccattg ggcgtttccg ggataaaatg
    15781 cctcactact tgcacctttc cggggcctaa ggaactccgg gacccggaac gcccaacttt
    15841 catattcgat tttcgatggt cgatctgaaa aaatctaaga tcgcaaactg taaactgtaa
    15901 attgtaaatt gtaaattcca gattctgctc ccgtatcctt tatccccgat tattccttta
    15961 tctttggcaa aattattttg aatgatatta attgcagatg ccggggcgac caagatccaa
    16021 tgggtggtca tccacgacgg aaaagccggc aatccttttg aaacgaatgg tttcaatccc
    16081 tacttcatgg cgccgaagat cctcgaagac gccgtggaaa aagacctggt accctttctg
    16141 agtcccccgc atatcaaaca gatctattac tacggcgccg gatgttcaac cgttcagaaa
    16201 tgcggtatcg tcgacgatgt gctgcgtgat atctttgaga atgccgatat cgatatccat
    16261 cacgacctgc tcggggccgc ccggtcattg ttcggaaaag tggaaggcat tgcctgcatc
    16321 cttggaacgg gatccaattc gtgcctgtac gacgggaaaa ccatcctgga gaatgtcact
    16381 tcgctgggct accttttcgg cgacgaaggc agcggcgcct acatgggcaa gctgttcctg
    16441 accgaatacc tcaggaaaag aatgccgcag catcttacag aagagtttga ccgcaaattc
    16501 ggcttcacgg tggaaaatat cctggattcc gtttataaca aacctcatcc caaccagttc
    16561 ctctcctctt tctcaaaatt tatcggggaa cataaagagg atgaatttat caaaacgatt
    16621 gcccggcaga attttcgtga ttttttcagg gaacaggtga cccggtacac tgattacaaa
    16681 caaaagccgc tgggtgtgat cggttccgtg ggttttcatt accaggagat attcagggaa
    16741 gtagcttcgg agcacggcct ggcagtttca aaactggtgc agtcaccgct gcagggcctc
    16801 gtggagtacc acgggggaaa ataattccgg gtcaatccag gtaacggttt tatccttcct
    16861 gaaccaggtc atctgccgtt tggcataccg ccgcgtattg atctttatct tttcagtcgc
    16921 ctcttccagg ctccattctc catcgaggta acagaatatt tccttgtagc cgacggtatt
    16981 cagtgcattc aggtgccggt atgaaaacag tcctttcact tcgtcgacca gtcccgcccc
    17041 gatcatctca tcggttcgcc tgttgatccg ctctgtcagc ttctcccttg aaatctccaa
    17101 ccctattttg acaatcctga acgaacggtc tttcagctcg cctttacggt aaccggaata
    17161 gggcttaccg gtgatcaggc aaacctctac agcccgcagc aggcgctgcg gattggaaac
    17221 atcgacggta ttgaaataac cgggatcgag tttctgtaat tgctcctgca ggtatcctat
    17281 gcctttctcc ctgtattttt ccgtcaattg tttccggatc tgcggattgt gctcgggttg
    17341 ttcatcgatc ccatggcaaa cggcatcgat ataaagtcct gatccaccgc acatcacgac
    17401 aacgtcgtgc ttttcaaaca acacctcaag caattcaatt gcatcgttct caaacctggc
    17461 aatgttataa tcttcggtta cggaaagatg gccgataaaa tgatgcctta cggcagataa
    17521 ttgctccggc gatggttttg ccgttccgat ggagagctcc ctgtaaaact gccgcgaatc
    17581 ggccgaaatg atctccgtat caagccattg cgctgcccgg atcgccaatt cggtctttcc
    17641 cgatgccgtc ggccctaaaa ttataagcaa tgttttcgcg acaggtgaac tcaaaactca
    17701 acattaaaca ttcaacattt ccttagtccc agtctctctc cttc
//
LOCUS       DAOPNH010000028        17610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3120_length_17610_cov_7.144973, whole genome shotgun sequence.
ACCESSION   DAOPNH010000028 DAOPNH010000000
VERSION     DAOPNH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_17610_cov_7.144973"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PKZ74_02710"
     CDS             complement(<1..1300)
                     /locus_tag="PKZ74_02710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM91693.1"
                     /translation="MEGRNNRALYFFLALAALLYLPAFLINLNMVQLIRDEAIRAIVA
                     FEMLQRNDFITPTIGGEPYLMKPPLFNWILAGFFQLTGSYSETVIRMPVIITIALYAG
                     TIYLFLRKPFGNLFALVNALAFVTYGRIIFYESLHGLIDLTFSWLTYCFFMLSWHFFE
                     RKKFLALFLVAYAITAVSYLLKGLPSLYFTAVTLLILFIQGKKFRMLFNWRHFLGIGL
                     LILVVGGYYLLYFSRNAIEPERVFEVLTGEVTRRTVVRFGIWKTILQLFTYPFENIYH
                     FLPWSALVILFIRKGSLKLIRENKRLWYMLLVFVFNIIPYWTSPETYPRYILMLVPLF
                     MTILFFLYFEYSKQDNWNKRAVDYILGIILGLAAIAAVIFLIHPATRDLPHVLPVSIG
                     LFITMSAISFFYWRKATFRLFWVIIAILVFRIAFNYTIIPS"
     gene            complement(1284..2300)
                     /locus_tag="PKZ74_02715"
     CDS             complement(1284..2300)
                     /locus_tag="PKZ74_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM91694.1"
                     /translation="MKRILLTGAAGFIGFHTVKKLVQAGHAVTGVDNINNYYDPELKY
                     ARLRECGIDRERIKELALIQSSTFPGYTFIKLDLKDREKTAQLFSGEPYDRVIHLAAQ
                     PGARHSFVDPYVYIDSNIEAFMNILEGCRHQRVKHLVFASSSSVYGNNPKQPSEETDF
                     VDHPISLYAATKKANELMAHTYSAAFGIPVTGLRFFTVYGPWGRPDMAYFKFARAIDE
                     GLPIEVFNGGNLERDFTYIDDIIKGLVKVTDIIPDGKIPYRLFNIGHSEPVNLLRFIE
                     IIEENLGKKAVMVMKPMQPGDVFSTYAGTGLLEKYAGYKPETGIEEGIKRFVDWYINY
                     GRKK"
     gene            complement(2470..3150)
                     /locus_tag="PKZ74_02720"
     CDS             complement(2470..3150)
                     /locus_tag="PKZ74_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91695.1"
                     /translation="MQNFELLQPGKFYHVYNCGINGEDLFRNNADFERFLRLFEKYIM
                     PVCETYAWVLMKNHFHFLVRIKENIGYKYSMNDFRDDKEKFNELKWETTNIVLTCRHP
                     EDLKVIKPTPHLHFSHLFNAYAKYFNIKYKRHGSLFERPFHRKKIDNKKYFKNVIVYI
                     HQNPVHHGFCDYPDEWGWSSYLTVISIKPTQLDREEVIGWFDNVGNFKDCHNNIIDRL
                     EFERYLGL"
     gene            3386..4330
                     /locus_tag="PKZ74_02725"
     CDS             3386..4330
                     /locus_tag="PKZ74_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM91696.1"
                     /translation="MPELSLVISVYNEEENILSLSEKIREALTGLDYEVIFVDDGSRD
                     KTVPMILSIVDPRVILLKFKKNYGQSSALSAGIDFASGDYIVTLDGDLQNDPADIPMM
                     LKVAREGDWDLVASNRKNRKDGMLIRKIPSRIANRIIRKTTGVKMKDLGCALKVFKKD
                     TAKNLGLYGELHRFIAVMAALDGATITQVDVNHFPRIHGQSKYGLSRTFKVISDLILL
                     LFIKRYLQKPMHFFGKWGLLILLPGVAINIYMLILKILGHDIWGRPLLILGIMLVVAG
                     FQMITSGIIAELVMRTYYESQQKKPYRVSNIFKGGKQA"
     gene            4314..5201
                     /locus_tag="PKZ74_02730"
     CDS             4314..5201
                     /locus_tag="PKZ74_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM91697.1"
                     /translation="MGSKLKRLLKTLLKFAVTAAALYFVIRKIELKEVLALYSQSNLV
                     YITLALIFFALSKLVSAYRLNIYFRTIDIHLKEKTNIELYLLGMFYNLFLPGGIGGDG
                     YKVYLLQKKYQAGTKKIIGAVLSDRISGMVALVVLALLGISILNPEVIGRWSLDVSRG
                     LLFSSVVIAIVTVFLAYYLFTKIAYPYFLKINILTFTYGFLVQLLQVACAWFLLLALG
                     EHENHLSYLVIFLVSSAVAVLPVSIGGMGVRELTFLYGSQILNVDMNVAVGISFLFYL
                     ITATVSLTGVWYIFRPVKL"
     gene            complement(5326..7338)
                     /locus_tag="PKZ74_02735"
     CDS             complement(5326..7338)
                     /locus_tag="PKZ74_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPM91698.1"
                     /translation="MKHLFYLAAILLVLSSCQFIPPKKIWLHDLDISMMEAGWGISRS
                     KLTVDSNQLIVAGKVYENGVGTHAISKMMIDFCDRSGGTSSAATGNKSCPSKAVRFSA
                     LVGIDDEANNERASAEFFVIGDQQILWRSGVMKFGDPPRKADVKLEGVKKLALYVSEA
                     GDFNYYDHTDWLEAFIEYRKESPVAISQPVPVAYILTPSAGEGPRINSPTVYGATPGK
                     PFLFRIPVSGRRPMNFSAQNLPGTLDLDVADGIISGTVPDSGTYVVNITVKNAIGEVS
                     RDLKIVTGQGLALTPPMGWNSWNCWGLSVDDAKVRAAADAFISSGLADHGWSYINIDD
                     GWEAPERNNKGEITTNEKFPDMKSLSGYVHSHGLKLGIYSSPGPLTCGGFLGSYEHEK
                     QDVKTWSDWGIDYLKYDWCSYGKIALNDSLDELQKPYLLMDKLLEDAPRDIVYSLCQY
                     GMGNVWEWGREVGGNLWRTTGDITDTWESMAGIGFTQDKCSPYATPGGWNDPDMLVVG
                     KVGWGPNLHPSKLTPDEQYTHISLWCLLSAPLLIGCDLSQLDEFTLNLLTNDEVLAVN
                     QDELGRQAVCMKRDSGYEIWSKTLSDGSVAVGIFNTGLGSPVDGFNWEAGGMPTKSIT
                     LNWEDLGLSGVYTVRDLWRQKDIGQFGSVYTAEVPYHGVVLVKLSR"
     gene            7484..7969
                     /locus_tag="PKZ74_02740"
     CDS             7484..7969
                     /locus_tag="PKZ74_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPM91699.1"
                     /translation="MKSTDKITFNARIEKHPGKDAGYIIFPHDVRQIYGVKGQVKVKA
                     VFDDLVHYRGSLARMGLPCHVLGITKEVRHQLEKTFGDEIKVEIEQDTDIREVEVPAD
                     VNALLEENPKARRFYTSLSYTDKKEFMRWIENARQSDTRTRRIGIFMEKMLVKKRFMD
                     L"
     gene            8005..8901
                     /locus_tag="PKZ74_02745"
     CDS             8005..8901
                     /locus_tag="PKZ74_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPM91700.1"
                     /translation="MPSQKQLDRIRKMIEPYCIKDGKGFKLKHFDPGDTGDFTKGDKE
                     IFKEALDEGVKILRELQEKLYADDRWSVLLIFQAMDAAGKDGAIKYVFSGINPQGCQV
                     FSFKAPSQEELDHDFLWRCTKNLPERGRFGIFNRSYYEEVLVVKVHPEFLSGQRLPKT
                     GNNIFEKRYTDINNFEKYLHRNGTIVRKFFLHVSKKEQKKRFLERLDNPDKNWKFSLN
                     DVKERQYWGQYMEAYEEMIRNTSTENAPWFVVPADNKWFARTIVAGAVIDALASLDLE
                     YPKLDKKKLADLDTARQALLNE"
     gene            9002..10630
                     /locus_tag="PKZ74_02750"
     CDS             9002..10630
                     /locus_tag="PKZ74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPM91701.1"
                     /translation="MKTLVSLILLTFLTATLTPVHSRDVPDAIKSKLEQLAVDKDLKI
                     AGEKIHSIEYITNLYQKNDYHPFWNKPEYILDAISGIRDSYNDGLLPGDYHLIAITTL
                     QSELTSPRGQTLPGPDQFADLDLLLTDGIIFLANHLLRGKIDPVSLLPTWNYGYAPIP
                     DLNALTLKGHITAREIPARLKELRPDMNLYDSLLTALEKYRRIAAQGGWPAIAGGGKI
                     EPGATDSRIPAIRNRLRLTGELTLRDSISSAKYDEPLEQDIKAFQASHGQFVDGIIGA
                     GTFRELNVPVEKRIESIRINLERVRWVAGNMPRKYILVNIAAFWLMMVDSNQVVHYTP
                     VVVGKSLSKTPVFRDKMRYIEFNPTWTQPRSIVKNETIPKLKKDSTYLEKNHMVLLDS
                     KGNLIETSSLDFKNLSPSKFPYIVRQEPGPWNSLGEMKFMFPNEYDIYLHDTPSKSLF
                     SRASRAYSHGCIRVKNPVDLAVKLLETTDHDRKKIMEILKTHETTRVNLPEPLDILLL
                     YWTCGIDRNNRLFFVPDIYERDADVLRELDQVMR"
     gene            complement(10627..11322)
                     /locus_tag="PKZ74_02755"
     CDS             complement(10627..11322)
                     /locus_tag="PKZ74_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HPM91702.1"
                     /translation="MFNLTAICLTLLLFVSNPKEFLDKSVPDTLKEDFTYLVEDYYNN
                     LYHETGLEGKLDYSIFRRALVGISLLDAPRKDILTIIDYTKPSSARRFFVIDLVNGKL
                     LYQTLVAHGKNSGEISCNRFSNREHSLQSSPGFFLTAESYSGRHGYSLRLDGMEPGLN
                     DQARERAIVIHGADYVCQRYVDDFGYIGRSFGCPALPLELNQTIIDLIKGGSCLYVHS
                     SDKNYFDLTTICD"
     gene            11515..12837
                     /locus_tag="PKZ74_02760"
     CDS             11515..12837
                     /locus_tag="PKZ74_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91703.1"
                     /translation="MKKIIFLIALKLSIIEGFSQSCLPDGIDFTNQSQIDNFQADYPG
                     CTEIEGDVEISGSGITNLNGLSVLTQIGGHLYISNNGSLDDLSGLSSLSFIGSSLLIS
                     NNPLLSNLNGINSLGVVEGDVFISNNPVLSDITALGGILPLLVSALRITGNPALSVCA
                     SPFICGFLANPTGKVIIYNNGAGCNNPPEIASQCGITLSCLPFGEYYFFSQDEINAFP
                     GNYPGCIDLKGTFHIEGTDITSLEPLFNIHSLDGTLEIHDNTSLPTLEGLNSLVAVDG
                     AISIVRNDLLTDISSLWQLDTGLISFLVISTNPLLSDCDIQSVCAYLAGPFGYSHNNI
                     TTNNSGCMDKDEVLQECSVNVEEFIFKENTLIITPNPASDKITIRSESPSPQSQISIL
                     NTNGQELFKQTVTGPVTEIDISCLPPGIYFVRLEGEKKLCNGRFVRLD"
     gene            complement(12913..13737)
                     /locus_tag="PKZ74_02765"
     CDS             complement(12913..13737)
                     /locus_tag="PKZ74_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPM91704.1"
                     /translation="MDNNQCYINNKFLDYPEAVLHISDLGLQRGYGIFDYFLEMDGRI
                     PFYEDYLDRFYHSAEMMHLQVPLTRELLKEKISFILKSNGFGRSGIKMLLTGGYSDDL
                     YSPVSSNFMIINMPMKHDPCEFSDGVKLLTLEYGRWWPEVKTTFYLPSLVMDKRLKDK
                     GAIEVLYYQNGLVTETTRANFFLVKDGILITAGDGVLKGITRKHVIQVGKEILPVEER
                     PVHLEEIRICDEAFITGTSKHIMPVVEIDEKVIGNGKPGDWTRKISEAFESYYKII"
     gene            complement(13852..15066)
                     /locus_tag="PKZ74_02770"
     CDS             complement(13852..15066)
                     /locus_tag="PKZ74_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM91705.1"
                     /translation="MNLELKKTAIRLILLFGIISLLGDIIYEGARGVNGPYLQTLGAS
                     AAIVGLVAGVGELLGYLIRLVSGYFSDKTKAHWFFTIFGYSFLMVIPLLSLTGVWQFA
                     AIFMVLERIGKGIRNPAKDTILSQATKQVGTGFGFAIVEFLDQIGATLGPLIFTFFFM
                     SIGPGERSVADYQQGYSLLWFPFVVLIMVLFTAFFMVRNPEELEKAVVKNPLPDKITR
                     TFWLYCIFTFVTTLGFLNFAIIGYHLKANNIVTDAQIPLMYALAMGVDAIIGLIIGKW
                     YDRLKSKHKNDHAGLLLLIALPLMTAPIPLLSLSPGLPFIITGVILWGIVMGTHETIM
                     KAGIADITSIRKRGTGYGIFNMLYGIAIFIGSAAAGFLYDYSINLLITLMIIVEMAAL
                     PVFFILRKHLLN"
     gene            complement(15165..15608)
                     /locus_tag="PKZ74_02775"
     CDS             complement(15165..15608)
                     /locus_tag="PKZ74_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPM91706.1"
                     /translation="MKKAGFSLLFIFLLFTGISQTIEKKIIQAPDAPKAIGPYSQAVM
                     AGNTLYVSGNIALNPENGQMDTLNLEAETRRVMSNLGAVLKEAGMSYENIVKATIYMT
                     DIRNYKEINRIYGEYFKEDPPAREAIQVVALPAGAHVEISCIAVK"
     gene            complement(15631..16926)
                     /locus_tag="PKZ74_02780"
     CDS             complement(15631..16926)
                     /locus_tag="PKZ74_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM91707.1"
                     /translation="MPINRRSFVRQFGATLGAASLGTALLPGFALGKEYEDPDYMNFP
                     PAGTPEDDFWAWVQQNYTVSPNIINLNNGGVSPSPRPVQDTFERYNRYCNEAPSYYMW
                     RILDQGREALRADLAALAGCSPGEIAINRNTTEAMDIVMYGLRLQKGDEVVLTKQDYP
                     NVINLWKMLEQRDGIVLKWVNLELPMEDDEALAQKYIDAFTGRTKVVNVTHIINWIGQ
                     IVPVKRIAAEAHKRGIDVLVDGAHTFGLLDFRIPDLDCDYFATSLHKWLGAPFGSGLL
                     YVKKEKISKIFPIYPDDKYMGDEIQKFERMGTRNFASEMAIGSALDFHLAIGSKRKEE
                     RLRYLKNYWMEQVKDIPGVNLHTSFKPEYSCVIGLFSIDGYTPGEIDTYLFDKHKIHT
                     VGIVWENISGVRVTPNVYTMERDLDKLVQGITQLAKQKK"
     gene            complement(17202..>17610)
                     /locus_tag="PKZ74_02785"
     CDS             complement(17202..>17610)
                     /locus_tag="PKZ74_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM91708.1"
                     /translation="KKADVVISNCVLNLVPDKEMAVSEICRVLKVGGHFCISDVIIKG
                     ELPEKIRNAAEMYAGCVSGALEREKYMELIARMGFINISIHKEKEILIPDNILSGYLS
                     KEEIEAYRKSGTGIFSITISGEKPAKGCVCCGT"
BASE COUNT         4619 a         4423 c         4025 g         4543 t
ORIGIN      
        1 atgaagggat aatcgtgtag ttaaaagcta ttctgaacac aagtattgct attataaccc
       61 aaaatagccg gaaagttgcc ttgcgccagt aaaaaaaact gatcgctgac attgttatga
      121 ataaaccgat tgataccggc aaaacatgcg gaagatcacg cgttgcaggg tgaataagaa
      181 atataacagc tgcaatggct gccaatccta agattattcc caatatataa tccacagctc
      241 ttttattcca gttatcctgt tttgagtatt caaaataaag aaagaaaagg atagtcataa
      301 atagaggcac cagcatgagg atataacggg gataagtttc aggcgaggtc cagtacggga
      361 tgatattgaa gacaaaaaca agaagcatat accacaggcg cttgttctcc ctgataagtt
      421 tcagtgagcc tttcctgata aatagaatga ccagggctga ccagggaagg aaatgataaa
      481 tgttttcgaa ggggtaggtg aacagttgaa ggatggtttt ccatatcccg aaacgcacta
      541 cggtccggcg tgttacttca ccggtcagca cctcgaatac tctttcgggt tcaatcgcat
      601 tgcggctgaa ataaagcagg taatatcctc cgacaaccag gatcagcaaa ccgatcccca
      661 ggaaatgccg ccagttgaaa agcatcctga actttttccc ctggatgaaa aggattaaca
      721 gtgtgacagc cgtgaaatac aggcttggca atccttttaa caggtatgaa acagccgtga
      781 tggcataggc aactaaaaac agggccagga atttttttct ttcgaagaaa tgccatgaaa
      841 gcatgaaaaa gcagtaggta agccacgaaa aagttaggtc gattaagccg tgcagcgatt
      901 cgtaaaatat tatcctgcca taggttacga atgccagcgc attgacaagg gcaaataagt
      961 tgccgaatgg cttccgcagg aacaggtaga tagttccggc ataaagagcg atcgtaatga
     1021 tgaccggcat ccggatcacc gtctccgaat aactgcccgt gagctgaaag aagccagcca
     1081 gtatccagtt gaacaacggg ggtttcatca ggtaaggttc cccaccgatg gtgggtgtga
     1141 tgaaatcgtt gcgctggagc atttcgaacg ccacgatggc gcggatggct tcatcgcgga
     1201 tcagttgaac catgttcagg ttgatcagga aagccggcag gtagagtaga gccgccagtg
     1261 ccaggaagaa ataaagtgcc ctgttatttc ttccttccat aatttatata ccaatcaaca
     1321 aatcttttta ttccctcttc tatgcctgtt tcaggcttat aaccagcgta tttttcgaga
     1381 aggccggtac ccgcatacgt tgaaaaaacg tcaccgggct gcatgggttt catgaccatc
     1441 accgcttttt tccccaggtt ttcttcgata atttcgatga accggagcag gttgaccggt
     1501 tcggagtgcc cgatattaaa cagacggtaa gggatcttcc catcagggat aatatccgta
     1561 acttttacca gccctttaat aatatcatcg atataggtaa aatcacgttc aagattccct
     1621 ccattaaaca cttctattgg caagccttca tcaatggccc gggcaaactt gaagtatgcc
     1681 atatcaggcc ttccccaggg gccgtaaacg gtaaagaacc tcagccccgt aaccgggata
     1741 ccgaatgcgg cgctgtaggt atgggccata agctcgtttg cttttttggt ggccgcgtac
     1801 aaactgatgg ggtgatccac aaaatcggtt tcctcggaag gctgtttcgg attattcccg
     1861 tagacgctcg aactgctggc aaagaccagg tgcttgaccc gctgatggcg gcatccctcc
     1921 aggatattca tgaacgcttc gatattgctg tcgatgtaaa catacggatc gacgaagctg
     1981 tgacgggcgc cgggctgcgc agccaggtgg atgaccctgt catatggctc cccggagaaa
     2041 agttgtgcgg ttttttcccg gtctttcagg tcgagtttga tgaaagtgta gccaggaaag
     2101 gttgaacttt gtataagcgc cagctctttg atccgctccc tgtcgatgcc gcactccctg
     2161 agcctcgcat atttgagttc gggatcgtaa taattattga tattatcgac ccccgtaaca
     2221 gcatggcctg cttgtaccag tttttttacc gtatgaaaac cgataaagcc ggcagcaccg
     2281 gtgagcagga ttcttttcat gctgacaaag atatttattt tataactctg tcaggtcttg
     2341 cggacgctgt tttcaacctg tcaggcactg cagtcgatga ttttaacctg tcaggtcctt
     2401 cggacgctga caggttaaat atccttcaaa cctgacggtg tccgcaggac ccgtcagcgt
     2461 ttgaacacat tacaacccca ggtatctttc aaactccaat ctgtcaatta tattattatg
     2521 gcaatcttta aaattcccta cattatcaaa ccatccaata acttcttccc tatctaattg
     2581 tgtcggttta atggagatta cagtcaggta cgatgaccat ccccattcat cagggtaatc
     2641 acaaaaacca tgatgaacag gattctggtg aatatagact ataacatttt taaagtattt
     2701 cttattatct attttctttc gatggaatgg tctttcgaaa aggcttccgt gtcgcttata
     2761 tttaatattg aaatatttcg cataagcatt gaacagatgt gagaaatgaa gatggggtgt
     2821 tggttttatc actttcaggt cctcaggatg ccgacaggtt aatacaatat tggttgtttc
     2881 ccatttcaat tcattaaatt tttccttatc atctctaaaa tcattcattg aatatttata
     2941 accaatattc tccttaatcc ttaccaaaaa atgaaaatga ttcttcatca acacccatgc
     3001 gtatgtctcg cagacaggca taatatactt ctcaaataag cggagaaatc tttcaaaatc
     3061 ggcattgttc ctgaaaagat cttctccgtt aattccacaa ttataaacat ggtagaattt
     3121 acctggttga agtaattcaa agttttgcat gtttccttcc tttctgctgt tgtaaccccg
     3181 tcaggtcctt cggacgccgt caggtttgaa actaattacc aggttggaat aatatggaag
     3241 gtctgtttag attatatcca ggcagctaag ataagaaaaa tgtcttcaat gaaaaatcat
     3301 ttttcgaaat ccttcaaacc tgacagcgtc cgaaggaccc gtcagcgttt gaaaaataaa
     3361 tatctttgca ccaaaaccag acaccatgcc tgaactctcc ctggtcattt cagtttataa
     3421 tgaggaagaa aatatccttt ccctgtcgga gaagatcagg gaagcgctga ccggtttgga
     3481 ttatgaggtg atctttgtgg atgatggctc cagggacaaa accgtgccca tgatcctgtc
     3541 gatcgtcgat ccccgcgtca tccttctgaa attcaagaag aattacggcc agagttcagc
     3601 cttgtcggcc gggatcgatt tcgcctccgg cgattatatc gtcaccctgg atggcgacct
     3661 ccagaacgat ccggccgata tcccgatgat gctcaaagtg gccagagagg gcgactggga
     3721 cctggtggcc agcaaccgga aaaaccgcaa ggacggcatg ctgatccgga aaataccgtc
     3781 gcgtatcgcc aaccggatca tccgtaaaac aaccggggtc aaaatgaaag acctgggttg
     3841 cgcactgaaa gtctttaaaa aggatacagc caaaaacctc ggattgtatg gcgaactgca
     3901 ccgctttatc gccgtcatgg cggccctcga cggcgccacg atcacacagg tcgacgtgaa
     3961 ccacttcccg cggatacacg gccagtcaaa atacggcctt tcaaggactt tcaaagtgat
     4021 cagcgacctg atcctgttgc tttttatcaa gcggtacctg cagaaaccga tgcacttctt
     4081 cggaaaatgg ggattgctga tcctgttgcc cggcgtggca atcaacatct atatgctgat
     4141 cctgaagatt ctgggacacg acatctgggg ccgcccattg ctgatcctgg gaatcatgct
     4201 ggtagtcgcc ggtttccaga tgatcaccag cggcatcatt gccgaactgg taatgcggac
     4261 ctactacgaa tcacaacaaa aaaagccgta ccgcgtcagc aatattttca aaggtgggaa
     4321 gcaagcttaa acgactgctg aaaacactgc tcaaattcgc tgtgactgcg gcggcattgt
     4381 actttgttat ccgaaaaata gaactgaaag aggtcctggc cctgtacagc cagtccaacc
     4441 tggtatatat taccctggct ctcattttct ttgctctttc gaaactcgta tcggcctaca
     4501 ggctgaatat ttatttccgc accatcgata ttcacctgaa agaaaaaacg aatattgaac
     4561 tataccttct cgggatgttt tacaacctgt tcctcccggg tggaatcggc ggagacggat
     4621 acaaggtcta tctccttcag aaaaaatacc aggccggaac gaaaaaaatt atcggcgcgg
     4681 ttctgtccga ccgcatttcc ggcatggtcg cgctggttgt gctggctttg ctgggtatat
     4741 ccattctgaa tcctgaagtc attggtcgat ggtcgctgga cgtaagtcgc ggattattat
     4801 ttagttccgt ggttattgcc attgtcacag tgttccttgc ttattacttg tttaccaaga
     4861 tcgcatatcc ctatttcctg aagatcaata tcttaacatt tacatatggc ttcctggttc
     4921 aattgttaca agttgcctgt gcctggtttt tactcctggc attgggtgaa catgaaaacc
     4981 acctctctta cctggttatt ttcctggttt catcggccgt ggctgttttg ccggtatcta
     5041 tcggtggtat gggggtcagg gaactgactt ttctttacgg gtcgcagata ctgaatgtcg
     5101 atatgaatgt agccgtcggg atcagcttcc tgttctacct gatcacggca actgtttcac
     5161 tgacaggagt ttggtatata tttcgaccgg ttaaactctg aaattccaaa ttccaaacac
     5221 caagcaccaa acaaattaga aattcaaaaa atcaaagtta agcgttttta attttaaatc
     5281 ttggtgtttg aatttgtttg ggatttggga tttggtgctt ggaatttacc tgctgagctt
     5341 caccagcacc accccgtgat aaggaacctc ggctgtgtat acgctgccga actgcccgat
     5401 atccttctgc cgccacagat ccctgacggt gtaaacgccg ctcaggccga gatcctccca
     5461 gtttagcgta atgctctttg tcggcattcc gccggcctcc cagttgaaac catccaccgg
     5521 cgaaccgagg ccggtattga atatcccgac tgcaacggaa ccgtcggaca gagtcttaga
     5581 ccaaatctcg taacccgaat cccgtttcat gcaaacagcc tgccgaccca gttcatcctg
     5641 gttcacagcc agcacttcat cattggtcag caggttcagg gtaaattcgt cgagctggct
     5701 caggtcgcag ccgatcagta gcggcgctga gagcaggcac cagaggctga tgtgcgtgta
     5761 ttgctcgtcc ggagtgagtt tgctggggtg caggttcggc ccccacccca ctttgccgac
     5821 caccagcata tccgggtcgt tccatccgcc cggggtggca taaggcgaac atttatcctg
     5881 cgtaaaaccg atcccggcca tactttccca ggtgtcggtg atgtcgcccg tggtacgcca
     5941 gagatttccc ccgacctccc tgccccactc ccagacattc cccataccgt actggcaaag
     6001 gctgtaaacg atatccctcg gagcatcctc aagcaattta tccattaaca ggtaaggttt
     6061 ctgcagctca tcgaggctat cgttcaacgc tatttttccg tatgagcacc agtcgtattt
     6121 caggtaatcg atcccccagt ccgaccaggt cttcacatcc tgtttttcat gttcgtagct
     6181 gcccaagaat ccgccgcagg tcagcggacc cggcgaagaa tagatcccga gtttcagccc
     6241 gtggctgtgg acgtatcctg acaacgactt catatccggg aatttctcat tggtagtgat
     6301 ttcgcctttg ttatttctct ccggcgcctc ccagccgtca tcgatattga tgtagctcca
     6361 gccatgatca gccaggccgg agctgatgaa agcgtcggct gcagcgcgca ccttggcgtc
     6421 gtctacgctc agaccccagc agttccaact gttccagccc atcggcggcg tcagggccag
     6481 tccctgcccg gtgacgatct tcaggtcccg ggatacctcg ccgatggcat tttttacggt
     6541 gatattcaca acataggtac cgctgtcggg caccgtgccc gaaatgatac cgtcagccac
     6601 atcgaggtca agggtcccgg gaaggttttg cgccgagaaa ttcatcgggc gccggcccga
     6661 aaccggtatg cggaatagga acggtttgcc cggtgttgcg ccgtaaactg tcgggctgtt
     6721 gatccgtggg ccttctcctg cagaaggtgt caaaatataa gctaccggca cgggttggct
     6781 gatcgccacc ggcgattctt tccggtattc tataaaagcc tcgagccagt cggtatggtc
     6841 gtaataattg aaatcgcctg cctcggaaac atacaacgcc agctttttaa ccccctcgag
     6901 ctttacatcg gccttgcgcg gaggatctcc gaacttcatc acgccgctgc gccagagaat
     6961 ctgttgatcg ccgatcacga aaaactctgc cgaggccctc tcgttattgg cttcatcgtc
     7021 aatacccacc agcgctgaga accggacggc cttggatggg caggatttat ttcccgtagc
     7081 ggcggaagat gttccgccgc tacgatcgca aaaatcgatc atcattttgc tgatggcatg
     7141 cgtaccgact ccgttttcgt atacttttcc cgctacaatc aactggttac tgtcaactgt
     7201 cagctttgat cttgaaatac cccatcccgc ctccatcatc gagatatcca aatcgtgcag
     7261 ccagattttt ttgggaggga tgaattggca ggaagaaagg accaataaaa ttgccgcaag
     7321 gtaaaacagg tgtttcataa agacaggatt tgattgatct gtaaaaatag gatattccgg
     7381 tgatttaaag aaacagtggc agaccgacgg atttccccgt catcctgcca aataatctaa
     7441 taattactaa ttttggaatc tggaacactg aaacagtcgc catatgaaat ccacagacaa
     7501 gatcaccttc aacgccagga ttgagaagca tcccgggaaa gatgccgggt acattatttt
     7561 tccccacgac gtcaggcaga tctacggggt taaagggcag gtgaaagtca aagccgtttt
     7621 cgacgacctg gtgcactacc ggggcagcct ggcaaggatg ggcctgccgt gccatgtgct
     7681 cgggatcaca aaagaggtgc gccaccagct cgaaaagacc tttggcgacg agatcaaagt
     7741 cgagatcgaa caggataccg acatccggga agtggaggtg ccggcagatg tgaacgcctt
     7801 gctggaagaa aatccgaaag cccgccggtt ttataccagc ctctcctaca ccgacaaaaa
     7861 agagttcatg cgctggatcg aaaacgcacg ccagtccgac acccgcaccc gccgcatcgg
     7921 tatcttcatg gaaaagatgc tggtaaaaaa gcggtttatg gacctctaat ccattatctt
     7981 tgtgcgatat taaccgattc tcctatgccc tcgcaaaaac aactcgaccg tatccggaaa
     8041 atgattgaac catactgtat taaggatggt aaggggttta aactgaaaca tttcgatccg
     8101 ggtgatacag gcgattttac caaaggcgac aaggagattt ttaaagaggc cctggatgaa
     8161 ggtgttaaaa tcctccgtga attgcaggaa aaactctatg ccgacgaccg ctggagcgtc
     8221 ctgctcatct tccaggcgat ggacgccgcc ggcaaagacg gcgccattaa atacgtcttc
     8281 tccggtatca acccgcaagg atgccaggtc ttttcgttca aagctccttc gcaagaggaa
     8341 ctcgaccacg acttcctgtg gcgttgcacc aaaaaccttc ccgaacgggg acggttcggc
     8401 atctttaacc ggtcgtacta cgaagaggtc ctcgtggtaa aagtacaccc cgaattcctt
     8461 tccggccagc gactgccaaa aacaggtaac aatatcttcg aaaaacgtta tacagacatc
     8521 aataactttg aaaaatacct tcaccgcaat ggaaccatcg tgaggaaatt cttccttcac
     8581 gtttctaaaa aagagcagaa aaaaaggttc ctcgaacggc tcgataaccc ggataaaaac
     8641 tggaaattct ctctcaacga cgtaaaggaa cgccagtact ggggacagta tatggaggca
     8701 tacgaagaga tgatccggaa tacatccacc gaaaatgctc cctggttcgt cgttccggcc
     8761 gataacaaat ggtttgcccg tactatcgtc gccggtgcgg tgatcgatgc tctggcctca
     8821 ctcgacctgg aatacccaaa gctagacaag aaaaaactgg ccgacctcga caccgcccgc
     8881 caggccctgc tgaatgaata atccagactc cgtaccctct gtgtactcca tggtaactaa
     8941 tagtccaaaa actccgtgcc cctctgtgaa actccatata ctctgtggtt aaaaaaacaa
     9001 tatgaaaact cttgtttccc tcatcctatt aacgtttctg actgctacac tcacccccgt
     9061 ccattccagg gatgtcccgg atgccatcaa atcaaaactg gaacaactcg ccgtggataa
     9121 agaccttaaa attgccggag aaaagatcca cagcattgag tatatcacga acctttatca
     9181 gaaaaacgat tatcatccat tctggaataa acctgaatat attctcgacg caatttccgg
     9241 tatccgggat tcatacaacg acgggctgct cccgggcgat tatcacctga tcgccataac
     9301 caccctgcaa tcggaactca catcccctcg cggtcaaacc ttgcccggcc cggatcaatt
     9361 cgccgatctc gacctgctgt tgaccgatgg cattatcttc ctggccaacc acctcctccg
     9421 cggtaaaatc gatcccgttt cattgttgcc aacatggaac tatggctacg cccctatccc
     9481 cgacctgaat gccttaacgc tgaaaggtca tatcaccgcc agggagatcc ctgcaaggct
     9541 gaaagaactc cgccccgata tgaacctgta cgattcgctg ctgacggcgc tggagaaata
     9601 ccgccggatt gctgcccagg ggggatggcc ggccatcgcc ggaggtggga agattgagcc
     9661 cggcgccact gattcccgta ttccggctat cagaaataga cttcgtctca ccggtgaact
     9721 gactctacga gactccatct catctgcaaa atacgatgag cctcttgaac aggatatcaa
     9781 ggcattccag gccagccacg ggcagttcgt cgacgggatc atcggtgcag gtaccttccg
     9841 cgaattgaac gtgccggtcg aaaaaagaat cgaatcgatc cggattaacc tggaacgggt
     9901 ccgctgggtc gccggcaata tgccccgaaa atatatcctg gtgaatatcg ccgctttctg
     9961 gctgatgatg gtggatagca accaggtcgt ccactatacg ccggttgtgg tcggtaaatc
    10021 attgagcaaa acacccgttt ttcgcgataa aatgcgctat atcgagttca atcccacatg
    10081 gacccagccc cggtcgattg taaaaaatga gacaattccc aagctgaaga aggattcaac
    10141 atacctcgaa aaaaatcata tggtgctgct ggattcaaaa ggcaacctga ttgaaacgtc
    10201 gtcacttgac ttcaagaacc tgagcccttc taaatttccc tatatcgtca ggcaggaacc
    10261 ggggccctgg aactcgctgg gcgaaatgaa gttcatgttc cccaatgaat acgatatcta
    10321 tcttcacgac accccttcca aaagcctgtt cagccgggcc agccgcgcct acagccatgg
    10381 ttgtatccgg gttaaaaatc cggtcgacct ggcagtgaag ctgcttgaaa ctactgatca
    10441 tgaccgcaaa aagattatgg aaatccttaa aacccatgaa acaacccgcg ttaatcttcc
    10501 cgaaccgctc gatatccttt tgctatactg gacctgcggt atcgacagga ataaccggtt
    10561 gttttttgtg ccggatattt acgaaaggga tgcagatgtt ttaagagaac tggaccaggt
    10621 gatgcgttaa tcgcaaatag tcgtaagatc gaaataattc ttgtcggagc tgtgcacgta
    10681 aaggcagctt ccccctttga taagatcgat aatggtttgg ttcagttcga gcggaagtgc
    10741 agggcaaccg aagctgcggc cgatataccc gaaatcatcc acataacgct ggcagacata
    10801 atcggcgccg tgaataacga tagcccgctc acgggcctgg tcattcagcc ccggttccat
    10861 cccgtcaagg cgcaatgaat aaccgtgccg cccgctgtat gactccgctg taagaaaaaa
    10921 tcccgggctg ctctgcaacg agtgttcccg gttggaaaaa cggttgcagc ttatctcacc
    10981 ggagttttta ccatgtgcta ccagcgtctg gtaaagcaat ttgccgttca ccaggtcaat
    11041 aacaaagaaa cggcgtgcac tcgacggctt ggtgtaatcg atgatggtca ggatatcttt
    11101 gcgaggggcg tcgagcaatg aaataccaac caatgccctc cggaaaatac tgtaatccag
    11161 cttcccttcc aggcctgttt catgataaag attattgtag tagtcctcca ccaggtaagt
    11221 aaaatcctct ttcagcgtat ccggcaccga tttatccagg aattccttcg gattgctgac
    11281 aaacagcaac aaagtcaggc agatggcagt gaggttgaac atggatttcc gaatcaattt
    11341 tcaggaagcc aaaaatagtt tttttcttat tgattttact attaattgat ccggcggaca
    11401 aatattctat tttactccgt gcaactctgt gcaaatctgt gtactcggtg gtgaaaacaa
    11461 tcttcagaac aattttatca ttatttttat tcaaaaaatt tttatccgga ttttatgaaa
    11521 aaaatcatct ttcttatcgc gttaaagctt tcgattattg aaggcttttc ccagtcctgc
    11581 ctgcccgatg gcatcgactt taccaaccag tcacagatcg acaatttcca ggcagactat
    11641 ccgggctgta ccgaaataga aggcgatgtg gagatcagcg gttcggggat caccaacctg
    11701 aacgggctca gtgtactgac ccagatcgga ggtcatctgt atatcagcaa taacggctca
    11761 ctggatgacc tcagcgggtt gagctctttg tcgtttatcg gcagctcgct gttgatcagt
    11821 aacaatccgc tcctgtcaaa cctcaatggc attaattcgc tgggcgttgt tgaaggcgat
    11881 gtgtttatca gcaataatcc ggtgctgagc gacattacag cactgggagg tatcttgcct
    11941 ttgttggttt ctgcactacg gataacaggc aacccggcac tgtcggtttg tgccagtcct
    12001 tttatatgcg gtttcctggc caaccccaca ggaaaagtca tcatatataa caatggcgct
    12061 ggatgtaaca atcctcctga aatcgcttca cagtgtggaa ttacattatc atgcctgcct
    12121 ttcggcgaat actacttttt cagccaggac gaaatcaatg cattccctgg taattatccc
    12181 ggctgcatcg atctgaaagg aacatttcat atcgaaggaa ctgacataac cagtctggaa
    12241 ccattgttca acatccattc cctcgatggt acccttgaaa tccatgacaa cacttcactg
    12301 ccaaccctgg aagggctcaa ttcgcttgtg gcagtcgatg gcgccataag catcgtcagg
    12361 aatgatctgc tgaccgacat ttcaagcctc tggcagctcg acaccggttt gatcagtttc
    12421 ctggtcatca gcaccaaccc attgctttcg gattgcgata ttcagtcggt ttgtgcttac
    12481 ctggccgggc cgttcgggta ttcgcacaac aatattacca ccaataattc gggttgcatg
    12541 gataaagatg aggtccttca ggaatgcagc gtaaatgtgg aagaatttat ctttaaggaa
    12601 aatacactga tcatcactcc caatcctgca tcggataaga tcactatccg gtcagaatcg
    12661 ccatcccctc aatctcaaat ttccattctc aacaccaacg ggcaagagct tttcaaacaa
    12721 actgtaactg gtcctgttac tgaaattgat atttcctgtc taccgccggg gatttatttt
    12781 gtaagattag agggagagaa aaagttatgc aatgggagat ttgtcaggct ggattaagat
    12841 tcttcgctcc gctcagaatg aagcagccag ttcgaaagag acaaatagcg gagaggaatc
    12901 tgttagaata aatcatatta tcttatagta gctttcaaaa gcctccgaga tcttgcgcgt
    12961 ccagtctccc ggttttccat tcccgattac cttttcgtcg atttccacaa ccggcataat
    13021 atgtttggat gtacctgtaa tgaaagcctc gtcacaaatc cggatttctt caaggtgaac
    13081 cggtcgctcc tctaccggca atatttcttt cccaacctgg atgacatgct ttcgtgtaat
    13141 tcctttcaac accccatctc ctgccgtaat caaaattccg tcttttacca ggaagaaatt
    13201 ggccctggtc gtttctgtaa caagaccgtt ttgataatat aaaacctcaa tggctccctt
    13261 atctttcaac cgtttgtcca taacgaggga tggaagataa aatgtcgttt tgacctccgg
    13321 ccaccatctt ccgtactcca atgtcagcag cttcacgccg tcgctgaatt cacacggatc
    13381 gtgcttcatc ggcatgttga tgatcatgaa attggacgaa accggcgaat aaaggtcatc
    13441 cgaatagcct cccgtgagaa gcattttgat ccccgatctg ccgaaaccgt tgcttttcag
    13501 aataaaggag attttctcct tcaatagttc tctggttaat ggcacctgca aatgcatcat
    13561 ctctgcagag tggtaaaatc ggtcgagata atcttcgtag aagggtatcc ggccgtccat
    13621 ctccaggaaa taatcgaaaa tgccataccc cctttgcagc cccaggtcgg aaatatggag
    13681 cacggcttcc gggtaatcga ggaatttatt gttgatatag cattgattat tgtccatagc
    13741 gattttaagt gttgaggttc ttactctgtg tttactctgt gtaaatccgt gtaatctgtg
    13801 gtgcaaaaga aatattcacc acagattaca cagagtttca ctaaggttat attaatttaa
    13861 caaatgtttt ctcaaaataa aaaataccgg taatgcagcc atctcaacaa ttatcatcaa
    13921 tgttatcaat aaattgatcg aataatcata taaaaatccg gcagccgcac tgccaatgaa
    13981 aatcgcaatc ccgtacaaca tgttaaagat cccgtaaccc gttccccgtt tccggatcga
    14041 cgtaatatcc gcaatacccg ctttcatgat ggtctcatgc gttcccatga caatgcccca
    14101 gaggatgact ccggtgatga tgaagggaag gcccggagac agggaaagga ggggaatagg
    14161 agcggtcatc aggggcaggg ctatgagcag gagcaatccc gcatggtcgt ttttatgctt
    14221 tgacttgagc ctgtcgtacc atttgccgat gatcaggccg atgatggcat cgacccccat
    14281 ggccagcgca tacatcagcg ggatctgggc gtcggtaacg atgttattgg ccttaagatg
    14341 gtatccgatg atggcaaaat tcagaaagcc gagggtggtg acaaaggtga agatgcaata
    14401 tagccaaaaa gtgcgggtga tcttatccgg caggggattc ttaacgacgg ctttctccag
    14461 ttcttccgga tttctgacca taaagaacgc tgtaaaaagc accattatca gtacgacaaa
    14521 aggaaaccag agcaagctgt aaccctgttg ataatctgca accgatcttt cgccggggcc
    14581 gatgctcatg aaaaaaaatg tgaatatcag cggccccagc gtagcgccta tctggtcgag
    14641 aaactccacg atggcaaaac caaaacccgt tccgacctgc ttggtggcct gcgacaggat
    14701 cgtatccttg gccgggttcc tgatcccctt cccgatccgc tccagcacca tgaagatggc
    14761 ggcgaactgc cacacgccgg taagcgacag taacgggatg accatcagga aactgtagcc
    14821 gaaaatggtg aagaaccagt gggctttggt cttatccgaa aaataaccgc tgaccagccg
    14881 gatgagatag cccagaagtt ctccgacgcc ggccaccaga ccaacaatgg cagcgctggc
    14941 gcccagggtt tgcaggtatg gcccgttgac tcctctcgcc ccttcgtaga ttatgtcgcc
    15001 cagcaggctg atgatcccga agagcaggat gaggcgaatc gcggtttttt tcagttcaag
    15061 gttcaaagtt gagtttaaat tataattttc ttccgacgac taaagccgtc ggttcatgct
    15121 tagtaatctc ggaaccaacg gctttagtcg ttggagcacg aaactcattt aaccgcaata
    15181 cacgaaatct ccacgtgtgc tccggccggc agtgccacca cctgtatggc ttcacgggca
    15241 ggaggatctt ctttgaaata ttcgccgtaa atccggttga tctctttgta attcctgata
    15301 tcggtcatat agatcgtggc tttgacaata ttctcatacg acatgcccgc ttctttcaat
    15361 acagcgccca ggttcgacat cacccttctt gtttctgcct ccaggttcaa tgtatccatc
    15421 tgtccgtttt ccggatttaa agcgatattg cccgaaacat agagcgtatt cccggccatg
    15481 acagcctggc tgtagggtcc aattgctttt ggcgcatccg gcgcctgaat gatcttcttt
    15541 tcgatggttt gagaaattcc tgtgaataac aggaagatga aaagcaaact gaaacctgcc
    15601 tttttcatgg ttaaaatata ttatcagatg ttattttttc tgctttgcca gttgggtaat
    15661 cccctgtacc agcttgtcga gatcgcgttc catggtataa acgttcggag taacacgaac
    15721 tccggaaatg ttttcccaga caatcccgac ggtgtggatc ttgtgcttgt cgaataggta
    15781 tgtgtcaatt tctcccgggg tatacccgtc gatggaaaac agcccgatga cgcaggaata
    15841 ttccggtttg aacgatgtat ggagatttac gcccggtatg tccttcacct gttccatcca
    15901 gtaattcttc agatagcgca gcctttcctc ctttcttttt gagccgatgg ccaggtggaa
    15961 atcgagcgcg ctgccgatag ccatctcgct ggcaaaattc cgcgttccca tccgctcgaa
    16021 tttctggatt tcgtcgccca tatatttatc gtcgggatag atcggaaaaa tctttgatat
    16081 cttttccttt tttacataca gcagacctga tccgaacggt gctccgagcc atttatgcag
    16141 gctcgtggca aagtagtcgc aatccaggtc aggaatcctg aaatcgagga ggccgaaggt
    16201 atgcgcacca tcaaccagga cgtcgatccc tcttttatgg gcttctgccg ctatcctttt
    16261 caccgggacg atctgcccga tccagttgat gatatgggtg acgttcacca ctttggtcct
    16321 gccggtgaat gcgtcgatat atttctgagc cagggcttcg tcatcttcca tgggcagctc
    16381 caggtttacc catttcaaca cgatcccgtc gcgctgttcc agcatcttcc agaggttgat
    16441 gacgttagga tagtcctgct ttgtaagcac tacttcatcg cctttctgca accggaggcc
    16501 gtacataaca atgtccatgg cctcggtggt attccggttg atggcaattt cgcccgggga
    16561 gcagccggcc agggcggcga ggtcggctcg aagcgcttcc cttccctggt cgaggatgcg
    16621 ccacatgtag tacgacggcg cctcattgca gtagcggtta tagcgctcaa acgtatcctg
    16681 caccggcctg ggcgaagggc tcacgccacc attgttcagg ttgatgatat tgggcgagac
    16741 cgtataattc tgctgtaccc aggcccagaa gtcatcttcc ggcgtaccgg ccggaggaaa
    16801 attcatatag tcgggatctt catattcttt gcccagggca aagcccggca ggagcgcagt
    16861 gcccagggaa gccgccccga gcgtggcgcc gaactgcctg acaaaactgc ggcggttgat
    16921 cggcattaaa gttagtatta aggttcaaat ttaggaaata aaaagaacga atgattcaac
    16981 aagtcagcca aaaaaagaat ctcatgagat ggcatctcag aataactgat attctcggtt
    17041 ataaatctgt ctttctgacg gttacaaagt tattttattg gatttgggat gccatctcat
    17101 tctaattcga cttccctccg gaaaggccct aaatcattga tcttccctct tacctaccgc
    17161 gagtaaatta ttgattttaa ttttaaaggc tttttttgac tttatgtccc gcagcaaaca
    17221 catcctttgg ccggtttctc acccgagata gtgatactga agattccggt acctgatttc
    17281 ctgtaagcct cgatttcttc ctttgaaaga taacccgaca atatgttatc cggaatcaat
    17341 atttctttct ctttatgaat agaaatgttt ataaatccca tcctggcaat gagttccata
    17401 tatttctccc gttcaagtgc accggaaaca catccggcat acatctcagc tgcattccgg
    17461 attttctcag gcaattctcc ttttatgatc acgtcggaaa tgcagaaatg ccccccgacc
    17521 tttagcaccc ggcagatttc cgacacagcc atttccttat ccgggaccag gttaagtaca
    17581 cagttgctga tcaccacgtc ggcttttttg
//
LOCUS       DAOPNH010000029        17608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3123_length_17608_cov_7.861676, whole genome shotgun sequence.
ACCESSION   DAOPNH010000029 DAOPNH010000000
VERSION     DAOPNH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_17608_cov_7.861676"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKZ74_02790"
     CDS             <1..231
                     /locus_tag="PKZ74_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91709.1"
                     /translation="VISTNIEVLRKFVTPEVGVLCSDNAEGFAIGLEKLYAARNQFDS
                     EYIRNKFKDYSIENVMKQNYLPYLEQIMNQPK"
     gene            244..1971
                     /locus_tag="PKZ74_02795"
     CDS             244..1971
                     /locus_tag="PKZ74_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91710.1"
                     /translation="MWLISNKSDENLSTLYHYRDFSEYYFYTDKAINQDAEAGIQWSC
                     DGHLFPRHPESIENGLFDQQSLLNELYTRHHSTFIDHIKGNFVIIQLEKSRFHIYSDR
                     FAVRKFFYWQHDQDFIITDDLKEIVKLVHPRPSIINMAVYALTYHFTGGTTLYENVKH
                     NVPGQVIEYTDGRLASDHYWKPDQLLHLQSRELDIKDIAHALANSTQSGLSLADRDQI
                     SLSLTGGADTRNLLATFLQKGIKPHLYTYGNPRSADCVKASAIARGLGLNHSIHDIKM
                     DENIFENYARKIVKKSGGLASIHRAHRLIAVEAEEQFAKWMFLGTLGGEFIKGVSEDD
                     YIVPPIVYDNWTNNQFTLDHLHRYYENKRLRYSHETAGQLLNFLAQEPYLTGDVISRK
                     FNALSHITAHLHDAQDVNLYSTVMEDVFTPFLDIDYLELLFSSDFTFNHKEQIKNKFL
                     KRMSNPVYGAEFIKATYKPLLKFKYSGEHKPSEVLFNKYTAAILKRIRQKTKPAYPPN
                     FPLGQWMEQFVAKHLPACADHEIIRETFDIDALLTDFKETVHFSKESYWLKFTNPIMM
                     RFILEEYNS"
     gene            1976..3025
                     /locus_tag="PKZ74_02800"
     CDS             1976..3025
                     /locus_tag="PKZ74_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91711.1"
                     /translation="MKVAVLQNEDPLSSQKWQQACEKSGIPCEIINLTASDWLSQVLN
                     EKYDFFLMKPPGNLSHFKTLYDERHYIISKVLKLKTFPSYEECFIYENKKLLSYFLEA
                     QGIPHPKTSVFYDLHEAHEFLSKTTFPLVAKTSIGASGSGVTIIRSKDNALKYVSKAF
                     SKKGIKRQFGPNRNTGTPGKWLGKALKEPRYFVKRLKVYFSIHKHGERDFVIFQEYIP
                     HAFEWRAVRIGDSYFAHQKVKVGDKASGSKGIDYVNPPESLLNFIKDLCETNRFNFMA
                     IDIFEDGKGGYLVNELQTLFGHVQDYILSIDGRIGRFRFLNNQWVFEEGNFNTNESYD
                     LRLETALSLYKNGNW"
     gene            3022..3975
                     /locus_tag="PKZ74_02805"
     CDS             3022..3975
                     /locus_tag="PKZ74_02805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM91712.1"
                     /translation="MKVLFVSSGNNKQGVGNVVKNQGDSLETHPEITLLSYYTIKGRG
                     IRGYLSNVIPLYKHLKNNEYNIIHAHYSLSALVVTLVSTRKLIVSLMGSDVYAKGVYR
                     FIIRLFSNFLWKATIVKSKEMQIKLMLPNAVTIPNGVDLSRFEYIEKTTAQLMTGFDK
                     SKTNIVFLADPNRREKNVILAEHAIKLLEDSNTALHIIYDIPHKNIREFLYAADILIL
                     TSLWEGSPNIIKEAMACNCPIVSTDVGDVKWLFGNTEGCYLTSFDPEDVAEKIRSALE
                     FAASKGRTNGRERMIELGLDADTVSQKLVMVYKNVLKDAKQ"
     gene            4058..5131
                     /locus_tag="PKZ74_02810"
     CDS             4058..5131
                     /locus_tag="PKZ74_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91713.1"
                     /translation="MVDKIKCIARHSKYCRKFFLCPDFIQDEFATFLIDLAEKEGVKG
                     WVLIPSNDHAVYTISKHKTRLEQYYKLITPGLDTIEKIYDKAALLSLAEQCNVPIPST
                     FYFKNENDPVPVSLAFPAITKGRNGLSFYRAMRMKALLARDEKELRKQLHIIHEKYNI
                     SGVFTQELIPFDGSNKTISFTAFCITGEIKSYWMGVKLREHPLQFGTATFAKSVYIQD
                     CYDLSIPLIKSINYTGVCEIEYLYDPRDKKYKLIEINARTWLWVGLAKFCGVNYARMI
                     YDHVNHNQNDFPATYDINKTWINPFSDLAYALLALVKGKITFKSYLSSFRNGAMVNAL
                     FLKNDWKPGVAYLLSLFSFLKKR"
     gene            5128..6303
                     /locus_tag="PKZ74_02815"
     CDS             5128..6303
                     /locus_tag="PKZ74_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM91714.1"
                     /translation="MKYPYANPNLRIADLIKTLFSSASASEERIKKYFSDLTGKKYIL
                     VTNSCRTALYMAYQSLGIKGEVITSPLTCKVALDPIIESGNTLVFADINTGDLNIVPG
                     DIEHRITDKTIAIQAIHLGGVACKMDRILSLAKQRNLIVIEDCAQSLGAYYNGKPSGS
                     FGDVSCFSLIKNAYGIGGGILATNSFEIYQKALKINEELLATGLMLLLFRTIRNLAET
                     NRNNILFRALHKSIMLVKGKKKSYGSVIQQLHQITCYEKKLAAHQLSRMTGLHNLRKN
                     IGKKYYELLTGNNILVNYDYEVNGSSFTKFFVYNPSLDSQKALTCLNAKGIEVMHLEQ
                     RYGNPLQPRFVDKETALLQGLKNYDTVHDCLISLPLPEGIKEKHIFIIVSALKEITN"
     gene            6310..7392
                     /locus_tag="PKZ74_02820"
     CDS             6310..7392
                     /locus_tag="PKZ74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HPM91715.1"
                     /translation="MKILLVTGHPAQVHNFKYLKSDLEARGHQVFWLATDKDISKYLL
                     DYYKIDYTVISRPGKSHFSKLRTLIKNTWFTKKFIRKNKIDLAFSRVSPYVALACFLL
                     HKTHFALTDTESAALYDKFFGRFVSVLFTSKSFKRTLRKDQVWFDANIELFYLHPNKF
                     KPLENVDNILNIKRDEPYIILRFVSWDAYHDKGLTGFTDINKLKAVTVFSKYAKVFIS
                     SEKELPAELAPFQIKIPPERMHDVLAHALLFFGESATMASESAVLGTPAIFLDKVGRG
                     YTDHEAKFELVYNFGNSLESQEKAINKGLELIQDQGLKNKMYHNHKRFMKDKIDVTAF
                     MVWFIENYPESDRIMQENPDYQYRFK"
     gene            7476..8552
                     /locus_tag="PKZ74_02825"
     CDS             7476..8552
                     /locus_tag="PKZ74_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HPM91716.1"
                     /translation="MSNCPTEMTYLFDLGHPAHFHLFKQSIRILQEKGHKAVITIKDQ
                     PVLIALLQDAGMEFINLGHKGKSLPAKALRQLMFDWKVWKIARKHRVDIGLGVSMSVP
                     HAALFCKMKSVLFDDDDRAATPLFYQFAHSVAGRVLSPDCLSFQQGGKKYTYYNGYHE
                     LAYLHPARFTPDPAILQKAGVNPGEPYFILRFNAFGAYHDTGQGGFSDEQKQDLIEKL
                     KPYGRVFITGEKDVAPQFRSYLINLHPADMHSFMAFATLFLGDSQTMASEAAMLGVPS
                     IRLNSFVGKISYLEEQEKKYGLTFGFLPQDFDKMLAKLDELLAMNDLKAEWQLRRRKM
                     LEEKIDVTAYLVEGLGAWGLGRRA"
     gene            8690..9979
                     /locus_tag="PKZ74_02830"
     CDS             8690..9979
                     /locus_tag="PKZ74_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91717.1"
                     /translation="MESTLILQNPHWKNIPYQGLLDREVIGEMIQLFKLKEILIIQGI
                     RRSGKSSVMYLMINKLLKDFPGDEILYINLEDPSFREAWEDPLHLRRLVEMASKITGK
                     IPQILLLDEIQLVKGWESYIKSVYDSLVFRKIIVTGSNQALLNSNYATSISGRYVSLQ
                     IFPFSLNERINHLGFGNRFDILSNKHHISRLLEEMMQFGGFPEICHTENAGLKRRQLI
                     GYFDSILMRDCLSIHAVRDVHLFKSLTTYYLSTIGRPYTYSSLARTVGSNEHTVRDYL
                     SFLTDAWLIKEIYPWSPAIKSLVSGPKKGYCSDNGLININSLSISPDFGWLFENLVFT
                     ELVQSGLTDIRFYSGYRECDFIVKAGQTLTAIQVCYNLNKNNQERETSGLIEAMEKTG
                     ADKGIIVSPFSSMEISDKVSVIPIFDIYKEMQTVDIG"
     gene            10115..10972
                     /locus_tag="PKZ74_02835"
     CDS             10115..10972
                     /locus_tag="PKZ74_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91718.1"
                     /translation="MEKPEPRSIILRHDVDQRPGYSLRFARMQHESGVKGSYYFRMVP
                     QSFNETIIKEILGLGHEIGYHYETMDTCKGDTEKALREFVRNLELLKKLAPIKTICMH
                     GSPLSKYDNRDLWGKEKSSVVSRQSSVISRQSSVVSGTREPGNPEPGTRNMPHYKDYD
                     IIGEPYFDIDFTQVLYLTDTGRRWDGAKVSVRDKVGSHQSAVSGRRSAVEQWNSGTMA
                     QSTRNPELKFHSTRDIIAAANAGLLPDRIMFTFHPQRWTDRPLPWLKELVMQNAKNIA
                     KRIILSSKS"
     gene            11084..12181
                     /locus_tag="PKZ74_02840"
     CDS             11084..12181
                     /locus_tag="PKZ74_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91719.1"
                     /translation="MKENPFKFGSIVDEPYFTDRILEIEQVKSIIGSQNHLTIISPRR
                     FGKSSLIMKVANQTNRPLIAIDMQLITSTSDLAAQILKRIYRIYPFEKVRQLVKNFRV
                     IPSITLNPLTNQVEVSFLPDSSQVPLLEDVLNLMEKLSSQKKKLLMIFDEFQETRRIH
                     PDLLRQLRSIMQYHKLINYIFLGSQESLIKEIFEMKKSPFYHFGMIIQLGKIPETDFF
                     NFLASRLEPVNKNASDLAQHIISYTGCHPYYTQQLAFVVWDKCYRKSFSENPVSEAAG
                     DLISMHDMDYERWWMNLNKTDKKLLIGLSLSNKTPLSEAFYRKNDLGAPSTVFSSIKR
                     LMVNGYIIKVDTKYEIDDPFFGQWLRLRREA"
     gene            12297..13565
                     /gene="wecB"
                     /locus_tag="PKZ74_02845"
     CDS             12297..13565
                     /gene="wecB"
                     /locus_tag="PKZ74_02845"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPM91720.1"
                     /translation="MKIISVVGARPNFMKIAPFIKAIDIHNTKFQIPNSKFQILNTQE
                     PIPKNQDQTPNTQYLSHLLVHTGQHYDIRMSGAFFRDLGIPEPDINLEIGSGTHAEQV
                     GMTMIEFEKVLQREKPDWVVVVGDVNATLACTVTAKKELVKVCHIEAGLRSGDMSMPE
                     EINRLVTDRLADLLLTPDRISIANLVKEGAAEHRIKMVGNIMIDTLEANRERAAGLEI
                     EDIIARNTIENSKFHGPAPTPSLPQLGEGEREGAVSFAVITLHRPSNVDREDVLVPLF
                     RFLADEVAGTMPLLWPVHPRAEKMLRQFGLWEEALAHPGLMMLHPLGYLEMLRLNMDA
                     RIMLTDSGGLQEECTVLGTPCLTMRWNTERPVTLREHGGASVLVGNSIDKIINEFLET
                     LQMPRKPMRPELWDGKTAARCLEEILSFKC"
     gene            13753..14598
                     /locus_tag="PKZ74_02850"
     CDS             13753..14598
                     /locus_tag="PKZ74_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91721.1"
                     /translation="MEARYGNALEMAKIQHEKGIRGTYYFRFLPGHFDTGIIREIAAM
                     GHEIGYHYDDLSYCKGNYEKAIRRFEKNLATLREIAPVKTICMEGAPLSKWDNRWLWG
                     KGVQSSKFKVQSSLNTAHRSPLTVHRSPLTVHRSPLTAHRSPFTAHYRDYGIIGEPYF
                     DTDFSDVFYLTDTGRRWDGKGSVRDKVGQLDSWAVGQLDNRTVGRHNPEPGTWNPELK
                     FHSTNDIIRAAEAGLLPDRIMFTFHPQRWTDKPLPWLQELVMQNVKNVAKRIVLRGVG
                     HRAQG"
     gene            14756..15880
                     /locus_tag="PKZ74_02855"
     CDS             14756..15880
                     /locus_tag="PKZ74_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91722.1"
                     /translation="MITENPFRYGVVVDDPYFINREKEVKEIGFDLISGNNLIITSPR
                     RYGKTSLVMKVINQLEKQGFPVIYLDFFRISDLKQFMDTYASQILRKQTGIKKSLAVF
                     QKWIHGIRPAITLDPSGSRSFTFSHDPAVPVYDSLTEILNLPLKINSDKRWIVVFDEF
                     QDIEKLNGQETEKWFRSVMQFHEQISYVFLGSKTHLIGQMFSQRERAFYGFGKLMRIG
                     KIPCIEMEHYISNRMNQTGINCDQASANQIISLADNIPYFVQFLASETWSLARENNGI
                     INDMLITRAVENILNNQQDHFHQLIDQLSPYQKKVVRALTSEQDQIYSQNFMKKYDLG
                     AVSSTQRAVESLTNSGILEKDQEAVYFSNPFFRQYLKDRA"
     gene            15991..16368
                     /locus_tag="PKZ74_02860"
     CDS             15991..16368
                     /locus_tag="PKZ74_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPM91723.1"
                     /translation="MKIILILYTIIIVLLAILPINNGNGFLNDNFILSFRLDYIAHFA
                     IFIPWMLISWLFYASWESRTLLRIFGWILLGLILAVATEFVQYLLPYRAFNINDLAAN
                     VLGVMLGGLFFIFKPKRKKEPEL"
     gene            16365..17519
                     /locus_tag="PKZ74_02865"
     CDS             16365..17519
                     /locus_tag="PKZ74_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPM91724.1"
                     /translation="MNFELITDPQRLLALGWDAFVRSHPQGTVFQSPDMFELFSRTAK
                     MTPVVIGVAEKGSGALRGILQGVVIREIKGPAGYFSSRTVIYGGPLVEAARQGDGETG
                     REGEVREIVGMLLRALIVVVKNRSIFIQFRNFTSQEDHREVFGENGFKYRDRLNLLVD
                     TSSFETVIQNMSGSRWRQIRKGLGQLDSWTVGQLDNRHPERNGRHPERSEGTPGHPAE
                     IIEPQNLQQVREFFNLLSDLYRYKVKKPLPEWDFFERFYNQSKEGKVGIIRLVKYRGK
                     IIGGILSPVTPGKTIYEWYVCGLDHEYKEVYPSVLATWAAIDYALKNGIPQFDFMGVG
                     IPEREYGVREFKSRFGGEMVNYGRFARINNKAVYMISEIGFNILALLKKI"
BASE COUNT         5182 a         3764 c         3899 g         4763 t
ORIGIN      
        1 gtcatttcaa ccaatataga ggtattgcga aaatttgtta ccccggaggt tggcgttctt
       61 tgttcagata atgcggaagg attcgctatc ggtttagaaa aattatacgc tgccagaaat
      121 caatttgatt cggagtatat tagaaataaa ttcaaggatt atagtattga aaatgtgatg
      181 aaacaaaatt atttgcccta cctggaacaa atcatgaacc aaccaaagtg aaattcataa
      241 gttatgtggc taatcagcaa taaatcagat gaaaatctga gcaccttata tcattaccgg
      301 gatttctccg aatattattt ttataccgac aaggcaataa atcaggatgc tgaagcggga
      361 attcaatgga gctgcgatgg ccatctattt ccgaggcatc ctgaaagcat tgaaaatggc
      421 ctgttcgatc aacagtctct tttgaatgaa ttatatacca ggcatcattc aacattcatt
      481 gatcatatca aggggaattt cgtcatcatt caacttgaaa aaagccggtt ccacatttat
      541 tccgaccgct ttgccgtccg gaaatttttt tactggcagc atgatcagga tttcattatt
      601 acggatgatc tgaaagagat tgtaaaactt gttcatccca ggccgtcaat aatcaatatg
      661 gctgtgtatg cactgaccta tcattttacc ggtggcacca cactttatga aaatgtgaaa
      721 cacaacgtgc ccgggcaggt catcgaatat actgacggcc ggttagcatc tgaccactat
      781 tggaaaccgg atcaactgct tcatttacaa tcccgggaac ttgatataaa agatattgcc
      841 catgcgcttg caaactcaac ccaatccggg ctaagtctgg cagatcgcga tcaaataagc
      901 ctttccctta ccggtggggc cgatacccgc aatttgcttg ccactttcct gcaaaaaggg
      961 attaaacctc atttatatac ctatggaaac ccacgctcag ccgactgtgt aaaagcttcg
     1021 gctattgccc gtggcctggg attaaatcac agcatccacg atataaaaat ggatgaaaat
     1081 atttttgaaa attatgcccg gaaaattgtc aaaaaaagtg gcggtctggc atcaattcac
     1141 agggcccacc ggttaatcgc cgtagaagct gaagaacaat ttgcaaaatg gatgttcctg
     1201 ggtacactgg ggggcgaatt tatcaaaggt gtgagtgaag acgattatat tgttccgccc
     1261 attgtttatg ataattggac aaataaccag ttcactttgg atcatctgca ccggtattat
     1321 gaaaacaaac gcctcaggta tagtcatgaa acggccgggc aattgttgaa tttcctggca
     1381 caggaacctt atctgacggg ggatgtgatc agccgcaaat tcaatgcact cagtcatatt
     1441 acggctcatc tgcatgatgc acaggatgtt aatttatact ccacagtaat ggaagatgtc
     1501 ttcacccctt ttcttgacat cgattacctt gaattgctct tttcgtctga tttcaccttt
     1561 aatcataagg aacaaatcaa gaataagttt ttgaaaagga tgagcaatcc tgtttatggc
     1621 gctgaattta ttaaagccac ctataagccc ttgttgaaat tcaaatattc cggggagcat
     1681 aaaccgtcag aagtgctttt caacaaatat accgctgcaa ttttaaaacg aataaggcag
     1741 aaaacaaaac ctgcttatcc gcctaatttt ccattgggac aatggatgga gcaatttgtg
     1801 gctaaacatt tgccggcatg tgcagatcat gaaatcatca gagaaacctt cgatatcgac
     1861 gccctgctga ctgattttaa agaaaccgtt catttctcca aagaatccta ttggttaaaa
     1921 ttcaccaacc cgattatgat gcggtttata cttgaagaat ataactcctg aatcgatgaa
     1981 agtagctgta ttacagaatg aagatcccct gtcttcgcag aaatggcaac aggcctgtga
     2041 aaaatccgga attccttgtg aaatcattaa tctgaccgct tccgactggc tcagtcaggt
     2101 tctgaatgag aaatacgact ttttccttat gaagccgcca ggtaatttat cccattttaa
     2161 gaccctatat gatgagcggc attacattat ttcaaaagtg ctgaaattaa aaactttccc
     2221 ttcttatgaa gagtgtttta tctatgaaaa caaaaagctg ctaagttatt ttcttgaggc
     2281 acagggaatt ccccatccaa aaacttctgt tttctatgat ctgcatgagg ctcatgaatt
     2341 tttaagtaaa acaacattcc ctttggtcgc aaaaacctcc atcggggctt cgggcagtgg
     2401 tgtaaccata atccggtcga aagataatgc gctgaaatat gtctcaaaag cattttcaaa
     2461 aaaagggatt aaacgacaat tcggacctaa ccggaatacc gggactccgg gaaaatggct
     2521 cggtaaagca ttaaaagaac ccagatactt cgtgaagcgg ttaaaagttt atttttcgat
     2581 tcataagcat ggtgaacgtg atttcgtcat ctttcaggag tacattccgc atgcatttga
     2641 gtggcgtgcc gttaggatcg gcgacagtta tttcgctcat caaaaagtta aagtgggtga
     2701 caaagccagc gggtcaaaag gaatcgatta tgttaatcca cctgaatctc tgctgaattt
     2761 tattaaagac ctgtgtgaaa cgaaccggtt caattttatg gccatagata tttttgaaga
     2821 tgggaaaggt ggttacctgg tgaatgaact ccagacgctt tttggccatg tgcaggatta
     2881 tatactatcc atcgatggaa gaatcggcag gttcaggttt ttaaacaacc aatgggtatt
     2941 cgaagaaggc aactttaata caaatgaatc ctatgattta aggcttgaaa ctgcattgag
     3001 cttatataaa aatggaaact ggtgaaagtt ctgttcgtta gcagcggaaa taataagcaa
     3061 ggtgttggca atgtggttaa gaatcagggt gattctttag agactcaccc tgaaattacc
     3121 ttgctttctt attatacaat taaaggccgc ggtataaggg gctatttaag caacgtgata
     3181 cctctgtata aacatctgaa aaacaatgaa tataatatta ttcatgcaca ttactctctt
     3241 tcggcactgg tggtcacatt ggtaagtacc aggaaattaa tagtctcgct gatgggaagt
     3301 gatgtttatg caaagggcgt gtacaggttc attattcgat tgttttccaa ctttttatgg
     3361 aaagcaacta ttgttaaatc taaggaaatg cagattaaac ttatgttgcc caatgccgta
     3421 acaattccaa atggtgttga cttatcaagg tttgaatata ttgaaaagac cacggcccaa
     3481 ttgatgaccg gatttgataa atcaaagacg aacatcgttt ttttagccga tccaaaccgc
     3541 agggaaaaaa atgtgatact ggcagaacat gcaataaaat tgcttgaaga ttcaaacact
     3601 gctttgcaca ttatttatga tataccgcat aaaaatatca gggaattctt atatgctgct
     3661 gatatattaa tcctcacctc tttatgggaa ggctctccca acatcatcaa agaagcaatg
     3721 gcctgcaatt gtcccatagt atcaaccgat gtcggtgatg tgaaatggct ttttggaaac
     3781 acggaaggct gttatctgac cagttttgat ccggaagacg tggctgaaaa aataagatcg
     3841 gccctggaat ttgcagcatc aaaaggaagg actaatggcc gggaacgcat gattgagctt
     3901 ggacttgatg ctgacactgt atcccaaaaa ttagtcatgg tttataaaaa tgttttaaaa
     3961 gatgcaaagc aataaacctg gcgccattat catcgaaggg catgtgcagg gcctgtcgaa
     4021 tacacgttcg ctgggtgagg ccggaatacc ggtatatgtg gtcgataaaa taaaatgcat
     4081 tgcccgccat tctaaatatt gcaggaagtt ttttctttgc ccggatttta ttcaggacga
     4141 atttgcaacg ttcctcatcg atcttgctga aaaagaaggt gttaaagggt gggttttaat
     4201 tccttccaat gatcatgccg tttacactat ttcaaaacat aaaacccgac ttgagcagta
     4261 ttacaaatta attaccccgg gccttgatac catcgaaaag atttatgata aagcagcgct
     4321 gctctcgctg gctgagcaat gtaatgttcc aattccatca acattttatt ttaaaaacga
     4381 aaatgatccg gtgccggttt ccttagcttt tccggccatt accaaggggc gcaacggact
     4441 atctttttat cgcgctatgc gcatgaaagc gctgcttgcc cgtgatgaaa aagaactgag
     4501 gaaacagttg catataattc atgaaaaata taacatttcc ggtgtattca cgcaggaact
     4561 catcccattt gatggaagca acaaaaccat ttctttcacc gctttctgta taactggtga
     4621 gataaaatca tactggatgg gtgtaaagct ccgtgaacat cccttacaat ttggaactgc
     4681 aacttttgcc aaaagtgttt atatccagga ttgctatgat ctttccatcc cgcttattaa
     4741 atcaatcaat tacacaggag tctgcgaaat agaatacctc tacgacccca gggataaaaa
     4801 atataagcta atagaaatca atgcaagaac ctggctttgg gttggattag caaaattttg
     4861 tggtgtgaat tatgcccgaa tgatctatga tcatgtaaat cataatcaaa atgactttcc
     4921 ggccacttat gatataaaca aaacctggat caatccattc agcgatttag cttatgcctt
     4981 gctggctctg gtgaaaggaa aaataacgtt caagtcttat ctttcatcct ttcgaaatgg
     5041 tgcaatggtg aatgcgctat ttctgaaaaa cgactggaaa ccaggtgtag cttacctttt
     5101 aagtctattt tcatttctaa agaaaagatg aaatatccct acgctaatcc taatttaagg
     5161 attgcagatt taattaaaac cctattttct tccgcatcgg cttctgaaga aagaattaaa
     5221 aaatacttct cagatctgac agggaaaaaa tatatcctgg tcaccaattc gtgccgcaca
     5281 gcactttata tggcttatca gagtctgggt attaaagggg aagtgatcac ctctcccctc
     5341 acctgtaaag tggctcttga tccaatcatt gaatcaggaa atactctggt tttcgcagat
     5401 attaatacag gtgatcttaa catagtaccg ggtgacattg aacaccggat cacagataaa
     5461 acaattgcga tccaggctat tcatctcggt ggggtcgctt gtaaaatgga tcgtattctg
     5521 tcccttgcaa aacaacgcaa tcttatagtg attgaggatt gtgcgcaatc gcttggggct
     5581 tattacaatg gcaaaccctc gggttcattt ggtgatgttt catgcttttc cctcataaaa
     5641 aatgcttacg gaattggagg aggtattctt gctaccaatt cttttgaaat ttaccagaag
     5701 gctttaaaaa tcaatgaaga actattggct acggggttaa tgcttttact attccgaacc
     5761 attcgtaacc tggcagaaac caatagaaac aacatccttt tccgtgcttt gcacaaatca
     5821 atcatgcttg taaaaggaaa gaaaaaaagc tacggttcag tgattcagca gttgcaccag
     5881 atcacctgct atgaaaaaaa attagcagca catcagctaa gccgaatgac agggctccac
     5941 aaccttcgaa aaaatattgg caaaaaatat tatgaattat taacgggtaa taacatcctg
     6001 gtaaattatg attatgaggt taatggttca tcgtttacaa aattttttgt ttacaatcca
     6061 tcgttagata gccaaaaagc ccttacgtgc ttaaatgcaa aaggaattga ggtgatgcat
     6121 ctggaacaga gatacggcaa tccattacag cccagatttg tagataaaga aactgccctt
     6181 ttacagggat tgaaaaatta tgataccgtt catgattgcc ttatttcatt acctctcccg
     6241 gaaggaatca aggagaagca tatcttcata attgtcagtg ccttaaagga aataactaat
     6301 taaaataata tgaaaatatt gttggttacc ggacatccgg cacaggtcca taattttaaa
     6361 tatttgaaat cagacctgga agcgaggggc catcaggttt tctggctggc aaccgacaaa
     6421 gacatcagta agtacctgct tgattattat aaaattgact acacggtaat ttcccgtccg
     6481 ggcaaatctc atttttccaa attacgtacc ctcattaaaa acacctggtt tactaaaaaa
     6541 tttatccgga aaaataagat cgacctggcc ttttcccgtg tatctcctta tgtagcatta
     6601 gcttgttttt tattgcataa aacccatttc gcgctaaccg acacggaatc tgcagcacta
     6661 tatgacaaat tcttcggtcg ttttgtttcc gtccttttta cttcaaaatc atttaaaagg
     6721 actttgcgca aagaccaggt ttggtttgat gccaatattg aattgttcta tcttcatccc
     6781 aataaattta aaccccttga aaatgttgat aatatcctca atatcaaaag ggatgagcct
     6841 tatattattc tgcgttttgt aagctgggac gcttatcatg ataaaggcct taccgggttt
     6901 acggatatta ataaactcaa ggcggttacg gtattctcta agtatgcaaa agtatttata
     6961 agttcagaaa aggaacttcc tgcggaactg gctccctttc aaattaaaat accgccggaa
     7021 agaatgcatg acgttcttgc ccatgcttta ttgttttttg gcgaaagtgc tacaatggct
     7081 tcagaaagtg ctgtattggg aactcctgca atatttcttg ataaagttgg aaggggatat
     7141 actgatcatg aggctaaatt tgaacttgtg tataattttg gaaattcctt ggaatcgcaa
     7201 gaaaaagcga taaacaaagg gctagaactt attcaggatc agggcttaaa aaacaaaatg
     7261 tatcataatc ataaaaggtt catgaaagac aagatcgacg tcaccgcctt catggtctgg
     7321 ttcatcgaaa attaccctga aagcgaccgc atcatgcagg agaaccctga ttaccagtac
     7381 cggtttaaat aatgagggaa atattgttcg attgttcgat tgttcgattg ttcgattgtt
     7441 caattgttca attgtttaac tataaaactg ttaaattgtc caactgccca actgaaatga
     7501 cctacctttt cgacctcggc caccctgcgc atttccacct gtttaagcaa agtatccgca
     7561 ttttgcagga aaaggggcat aaggcggtca ttacgatcaa ggatcagcct gttttgattg
     7621 cacttttgca ggatgcgggg atggagttca tcaacctggg acataaagga aaatcgctgc
     7681 ctgcaaaggc gcttcgccag ctcatgttcg actggaaagt ctggaaaatc gcccggaagc
     7741 accgcgtcga tattggcctg ggcgtttcca tgtcggtgcc tcacgccgcc ctgttctgca
     7801 aaatgaaatc ggtattgttc gacgacgacg accgggccgc gacgcccctg ttctaccaat
     7861 ttgcccacag cgtggccggc cgggtgctgt cgcccgattg cctgagcttt cagcagggcg
     7921 gaaaaaaata tacgtattac aacgggtacc atgagctggc ctacctccac cccgcccgct
     7981 tcacccccga cccggccatc ctgcaaaaag ccggcgtaaa cccgggcgaa ccctatttta
     8041 tcctgcgttt caatgccttc ggggcctatc atgacaccgg gcagggcggc ttttcagacg
     8101 aacagaaaca agatttgatt gaaaaattaa agccttacgg cagggtgttc attaccggcg
     8161 aaaaagatgt ggcgccacag ttccggtctt atcttataaa cctccacccc gccgatatgc
     8221 atagttttat ggcattcgcc acgctattcc tgggtgatag ccagaccatg gcctccgagg
     8281 ctgctatgct gggcgtgcct tctatccggt tgaactcgtt cgtgggaaaa atatcgtacc
     8341 ttgaagaaca ggaaaagaaa tatggcctga cctttggctt tctgccacag gatttcgata
     8401 aaatgcttgc taagctcgat gagttgctgg caatgaatga tctgaaagca gaatggcagc
     8461 tacgcaggcg gaaaatgctg gaggaaaaga ttgatgtgac ggcgtacctg gttgaggggc
     8521 ttggggcttg gggcttgggg cgtagggcgt agggcgtagg gttgatggtt aagtttggga
     8581 atgtattaag acccgaaggg tcgaatatga atagcgaaag taatgagatt gatcgcgagt
     8641 tatgtttcgg gataattagt atatttgcga attataattc gtccatatta tggaaagcac
     8701 gttaatcctg caaaatcctc actggaaaaa cattccctac cagggattgc tcgaccgtga
     8761 agtgatcggg gaaatgattc agctatttaa gctgaaagaa atcctcatca tccagggtat
     8821 cagaagatcc ggaaagtcat cggtcatgta tttaatgatt aacaagttac ttaaagattt
     8881 tcctggcgat gaaattcttt atataaacct tgaggatcct tcgttcagag aggcctggga
     8941 agatccgctg catctgcgac ggctggttga gatggcttca aaaattaccg gaaaaatacc
     9001 acaaattctt ttactggatg aaattcagct tgtaaaaggc tgggaatctt atattaaaag
     9061 tgtttatgac agccttgtct tccggaaaat aattgttaca ggctcaaacc aggccttgtt
     9121 aaattcgaat tatgccacaa gcatttccgg ccgctacgtc agtttgcaga tattcccttt
     9181 ctctttaaat gaaaggataa accaccttgg ctttggtaat cgatttgata ttttatcaaa
     9241 taaacatcac atcagcaggt tattggaaga aatgatgcaa ttcggagggt ttccggaaat
     9301 atgccatact gaaaatgccg ggttaaagag aagacaactc attggttatt ttgattccat
     9361 attaatgcgc gattgccttt ctatccatgc agttcgtgat gtccatcttt ttaaatccct
     9421 tacaacttac tatttatcaa ctattggccg gccatatacc tattcaagcc tggcccgcac
     9481 tgtgggcagc aatgaacata ccgtaaggga ttacttgtct tttttaacag atgcctggct
     9541 gataaaggaa atctatccat ggtcgcctgc cataaaatcc cttgtatcag gtccgaaaaa
     9601 agggtattgc tctgataacg gactgataaa tattaattcg ttgtcaatat cccctgactt
     9661 tggctggctt tttgaaaacc tggttttcac agagttagtg caaagcggat tgactgatat
     9721 ccgtttttat tccggctatc gtgaatgtga ttttattgta aaagccggac aaacccttac
     9781 ggcaatccag gtatgttata atttaaacaa aaataaccag gagcgggaaa cttcaggatt
     9841 gatcgaagcg atggagaaga ccggggcaga taaaggtatt atcgtttcac ctttttcatc
     9901 gatggaaata agtgataaag tcagcgtaat accaatattt gatatataca aagaaatgca
     9961 gactgttgat atcgggtaga agggtgtgcc cgtatccttt accagtttaa caatttaaca
    10021 atttactacc acggacttca ccatcaaaat ataccgtaag ctacttgaat cactcatcga
    10081 tcagggctat gctttccaga ccttcgctga attcatggaa aaaccagagc cgcggagcat
    10141 cattcttcgg cacgacgtgg accagcggcc cggatacagc ctgcgttttg cccggatgca
    10201 gcatgagtcg ggcgtgaaag gctcctatta cttccgcatg gtgccgcaaa gttttaatga
    10261 aacgataatt aaggaaatat taggtcttgg ccatgagatc ggctatcatt acgaaacaat
    10321 ggatacctgc aaaggtgata cagaaaaagc gctaagggaa ttcgttcgga atcttgaatt
    10381 gttaaaaaaa ctggccccaa ttaaaaccat ctgcatgcac ggtagcccgc tatcaaaata
    10441 cgacaaccgg gacctgtggg gaaaagaaaa gtcatcagtc gtcagtcgtc aatcatcagt
    10501 catcagtcgt cagtcgtcag tcgtcagtgg aacccgggaa cccgggaacc cggaacccgg
    10561 aacccggaac atgcctcact acaaggatta cgacatcatc ggcgagcctt atttcgacat
    10621 cgatttcacg caggtgcttt acctcaccga tacgggacga agatgggacg gggcgaaggt
    10681 gagtgtaagg gataaagtcg gcagtcatca gtcagcagtc agcggtcggc ggtcggcagt
    10741 ggaacaatgg aacagtggaa caatggcaca atcaacccga aacccggaac tgaaatttca
    10801 cagcacccgc gacatcatcg ccgccgccaa cgccggtctc ctccccgacc ggatcatgtt
    10861 cacgttccac ccgcagcgct ggaccgaccg gccgctgccg tggctcaaag aactggtgat
    10921 gcaaaacgca aagaacattg caaagagaat aatcctaagt tctaagtcct aagttctaag
    10981 ttttaagtcc taaagagttc cgtcaggaac gaaattatgg ttgggatatg aatacttttt
    11041 attaattttg cgaattttat cgcaacgtaa ttattcgcaa taaatgaaag agaatccttt
    11101 taaatttggc agcattgtcg atgaacccta tttcactgac cgcattcttg aaatagaaca
    11161 ggtgaaatcc attattggca gtcaaaacca tcttaccatc ataagtccca gaaggttcgg
    11221 gaaatcaagt cttatcatga aagtggccaa tcaaaccaac cggccattga ttgcaatcga
    11281 catgcaattg attacaagta catcagacct tgcggcccag atccttaaac gcatttacag
    11341 gatttatcct tttgaaaaag tcaggcagct tgttaaaaat ttccgtgtta ttccttccat
    11401 tacacttaat ccgctaacta accaggttga agtgtcattt ctccccgaca gttcccaggt
    11461 gcctttgctt gaagatgtac tgaacctgat ggagaaatta agctctcaaa aaaagaaatt
    11521 attgatgata ttcgacgaat tccaggaaac aagacgcatt catcctgatt tattgaggca
    11581 gctaaggtcc attatgcaat atcataaact tattaattac atttttcttg gaagccagga
    11641 atcattaata aaggagattt ttgagatgaa aaagtctccc ttttatcatt ttggcatgat
    11701 tattcagctt ggaaagatcc ccgaaacaga tttttttaat ttcctggcaa gccggcttga
    11761 gcctgttaat aagaacgcat ctgatctggc tcagcatatt atcagttaca caggctgcca
    11821 tccgtattat acgcaacaac tggcctttgt ggtttgggat aaatgctaca ggaaatcatt
    11881 ttccgaaaat ccggtttctg aagctgccgg tgatttaatc tcgatgcatg atatggatta
    11941 cgagcgatgg tggatgaatt tgaataaaac agataaaaag cttcttatcg gattgtcact
    12001 ttcaaataaa accccgcttt cggaagcatt ttacagaaaa aatgaccttg gtgccccaag
    12061 tacagtattt agcagtatca aaaggctgat ggtaaacggt tatataatta aggtcgatac
    12121 aaaatatgaa atcgacgacc cgttcttcgg acaatggcta aggttgagaa gggaagcgtg
    12181 agaaaagtcc taagttctaa gtcctaagtc ttaagttttg agttttgagt tcgggattcc
    12241 gagggcacac ccgtatccct taacagttta acagtttaac aatttaacag ttttccatga
    12301 aaatcatctc cgtcgtcggc gcccgtccta actttatgaa gattgcaccc tttataaaag
    12361 ccattgatat acacaatact aaattccaaa ttccaaattc caaattccaa attctaaata
    12421 cccaggaacc aatacccaag aaccaagacc aaacacccaa tacccaatac ctaagtcatc
    12481 ttctcgttca caccggccag cattacgaca tccgtatgtc gggggctttt ttccgcgacc
    12541 tgggaatccc ggagcccgat atcaacctgg agatcggttc gggaacacat gctgagcagg
    12601 tggggatgac tatgattgaa ttcgaaaaag tgctgcagcg ggaaaagccc gactgggttg
    12661 tcgtagtcgg agatgtgaac gcaacacttg catgcacggt cactgcaaag aaagaactgg
    12721 tgaaagtctg ccacattgaa gccggcctgc gatcgggcga tatgagcatg cccgaagaga
    12781 tcaacaggct ggtcaccgac cgccttgccg acctgctcct cactcctgac cggatttcca
    12841 ttgccaatct tgtaaaagaa ggtgcagccg aacaccggat caaaatggtc ggaaacatca
    12901 tgatcgacac gctcgaggca aaccgggaac gggctgcagg gctggaaatt gaggatatta
    12961 ttgccagaaa taccattgaa aattccaaat tccatggacc agcccccacc ccgtccctcc
    13021 cccaattggg ggagggagaa agggaggggg ccgtctcatt cgctgtaatc actcttcacc
    13081 gcccttccaa cgtcgaccgt gaagacgtcc tggttccttt gttccgtttc ctggctgatg
    13141 aagttgccgg cacgatgccc ctcttatggc cggtgcatcc ccgcgctgaa aaaatgctgc
    13201 ggcagttcgg cctctgggaa gaagcgctgg cccatcccgg cctgatgatg ctccacccgc
    13261 tgggctacct ggaaatgctg cgacttaata tggatgcccg aattatgctt accgacagtg
    13321 gtggattaca agaagagtgt actgttcttg gcaccccttg tcttacaatg cgctggaata
    13381 ccgaacggcc ggtgacactg agagaacacg gtggtgccag tgttctggtg ggtaattcta
    13441 ttgataaaat aataaatgaa ttcctggaaa cgcttcaaat gccgcgtaag ccgatgcgac
    13501 cagagttgtg ggacgggaaa acggcagccc gatgcctgga ggaaatttta agttttaagt
    13561 gttgagtctt aagttctgga atcttttttt acaattttac aatttaacag tttaacagta
    13621 acaaccacgg acttcactat aaagatatac cgctccctcc ttgaatccct tgccgcgcag
    13681 ggatactctt tccagcccta cgccggattc ctcgaaaatc cggcttcccg ctgcataatc
    13741 ctccgccacg acgtggaggc gcgctacgga aatgcgctgg aaatggcaaa gatccagcat
    13801 gaaaaaggga tccgcggaac ctactatttc cgcttccttc cggggcattt cgataccggt
    13861 atcatccgcg agatcgccgc tatgggccac gagatcggct accattacga cgatctttcc
    13921 tattgtaaag gcaactatga gaaagcgatc aggcgcttcg aaaaaaacct ggccaccctg
    13981 cgtgagatcg ctcccgtgaa aaccatttgc atggaaggag caccgctgtc gaagtgggac
    14041 aaccgatggc tgtggggaaa gggagttcaa agttcaaagt tcaaagttca aagttcactt
    14101 aataccgctc accgttcacc gctcaccgtt caccgttcac cgctcaccgt tcaccgttca
    14161 ccgctcaccg ctcaccgttc accgttcacc gctcactacc gcgattacgg catcatcggc
    14221 gaaccctatt tcgataccga tttctcggat gtcttctacc tgaccgacac cggccggcga
    14281 tgggacggga aaggcagtgt gcgggataaa gttgggcagt tggacagttg ggcagttgga
    14341 caattggaca atagaacagt cggaagacac aacccggaac ccggaacctg gaacccggaa
    14401 cttaaattcc acagcacaaa cgacattatt cgtgctgccg aagccggcct gctgcccgac
    14461 cggatcatgt tcacgttcca cccgcagcgc tggacggata agccgctgcc atggctgcag
    14521 gaactggtga tgcagaatgt gaagaacgtg gcaaagagaa tagtgttgag gggcgtaggg
    14581 catagggcgc agggctaagt gttgagggtt atgttcggga atgtatgaag acccgaaggg
    14641 tcaaaaatga atagcgtcag gaacaacagt ttggtagccg ggaatttttt ttgcaaatca
    14701 tttgaagaag tcataattat ttgtatcttg cgttacgcaa ataagcataa tatcaatgat
    14761 cactgaaaat cctttcagat atggtgtcgt agttgatgat ccgtatttta ttaaccgcga
    14821 aaaagaggtt aaagaaatcg gttttgacct gatttccggt aataatctca ttattacttc
    14881 gccaaggcga tatggtaaaa cttcgcttgt gatgaaagtc atcaatcaac ttgaaaaaca
    14941 aggatttccg gtcatttacc tggatttctt ccgcatctct gacctgaaac aattcatgga
    15001 tacttatgct tcacaaattc tcaggaagca aacaggcata aaaaaatccc ttgccgtatt
    15061 tcagaagtgg atacacggta ttcgtccggc cattacgctc gacccttctg gttctcgttc
    15121 atttactttt tcgcatgatc ctgcggtacc ggtatatgat tccctgacag aaatcctgaa
    15181 cctgcctttg aaaattaaca gcgataaaag atggattgtg gtttttgatg aatttcagga
    15241 tattgaaaaa ctgaatgggc aggaaactga aaagtggttc cgttcggtca tgcaatttca
    15301 tgaacagatc agctacgtat tccttggcag taaaacccat ttgatcggac aaatgttttc
    15361 tcagcgggaa cgggcattct acggatttgg aaagctgatg cgaattggaa aaatcccttg
    15421 cattgaaatg gagcattaca tttcaaacag aatgaatcaa accggcataa attgcgacca
    15481 ggcttccgcc aaccaaatta tttctttagc tgacaacatt ccatactttg tgcagttcct
    15541 ggcttcagaa acatggtccc tggcccgtga aaataatggc attatcaatg atatgctgat
    15601 tacccgggct gttgaaaata tactgaataa ccagcaggat cattttcatc aactcatcga
    15661 tcagctttca ccttatcaga aaaaagtcgt tcgcgccctg acttcagaac aggaccagat
    15721 ctattcacag aattttatga aaaaatatga ccttggcgcc gtcagcagta cacaaagggc
    15781 tgttgaaagc ttaaccaact ccggcattct tgagaaagac caggaagcgg tttatttttc
    15841 caatccattc ttcaggcaat atttgaaaga ccgggcatag ggcgtagggc atagggttaa
    15901 gttcgggatt gtattaagac ccgaagggtc aaatatgaat agcgtcaaag taaagtataa
    15961 ctttcatata tcatgtatat gtaaaccgta atgaaaataa tcctgatact ctataccatc
    16021 atcatcgtcc tgctggccat cctcccgatc aacaacggca atggattcct gaacgacaat
    16081 ttcatccttt cattccggct cgattacata gcccacttcg cgatatttat cccctggatg
    16141 ttaatctcgt ggctctttta tgcctcctgg gaaagcagga ccttacttag aatcttcggt
    16201 tggatattgc ttggattgat ccttgcggtt gccacagaat ttgtacaata tttgctccct
    16261 taccgcgcct tcaatatcaa cgacctggct gccaacgtgc tgggcgtcat gcttggaggc
    16321 ttgtttttta tttttaaacc gaaaaggaag aaggaacctg aactttgaac tttgaactga
    16381 tcaccgatcc tcaacgtctt cttgcgctcg ggtgggatgc attcgtcagg tctcacccgc
    16441 aggggacggt gttccagtcg cccgacatgt ttgaactttt cagcaggaca gcgaagatga
    16501 cgccggtcgt gatcggggtt gcggaaaaag gttccggtgc actcaggggg attctgcagg
    16561 gtgttgtgat ccgggaaata aaaggtccgg ccgggtattt ttcgtcgcga accgtgattt
    16621 acggagggcc gcttgtagag gcagcgagac agggagacgg ggagacaggg agagaggggg
    16681 aagttcggga gattgtgggg atgctgctgc gggctttgat cgtggtggtt aaaaaccgct
    16741 cgatcttcat ccagttccgg aatttcacca gccaggagga tcaccgggag gttttcgggg
    16801 aaaatggatt taaataccgc gacaggctga acctgctggt ggatacatct tcttttgaaa
    16861 cagtgatcca aaatatgagc ggatcgaggt ggaggcagat caggaaaggc ctggggcagt
    16921 tggacagttg gacggttgga cagttggaca accgtcatcc tgagcggaac ggacgtcatc
    16981 ctgagcggag cgaaggaact cctggccatc ctgccgaaat catcgaaccg caaaacctgc
    17041 aacaagtcag ggaattcttc aatctcctgt cagaccttta caggtacaaa gtcaaaaagc
    17101 ccctgccgga atgggatttc ttcgaacggt tctataacca gtcgaaagaa ggaaaagtgg
    17161 gtatcatccg cctggtgaaa taccggggaa agatcattgg cgggatactt tcacctgtca
    17221 ccccgggaaa gacaatttac gaatggtatg tctgcgggct ggatcatgaa tacaaagagg
    17281 tttatcccag cgtactggct acctgggctg ccattgatta tgccttgaag aacgggatcc
    17341 cacagttcga tttcatgggt gtagggattc cggagcggga atatggcgtg agggagttca
    17401 agtcgcggtt cgggggtgaa atggtgaatt acgggcgatt tgcacggatc aacaacaagg
    17461 ccgtttacat gatttcggaa attgggttca atattctggc tttactgaaa aagatttgat
    17521 gttggcttaa aacttgattt gttagtccga cgggtgaatc cgttggttgc cagtgatcgt
    17581 gctccaacga ctaaagccgt tggttcca
//
LOCUS       DAOPNH010000030        17521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3149_length_17521_cov_7.198843, whole genome shotgun sequence.
ACCESSION   DAOPNH010000030 DAOPNH010000000
VERSION     DAOPNH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3149_length_17521_cov_7.198843"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKZ74_02870"
     CDS             <1..508
                     /locus_tag="PKZ74_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPM91725.1"
                     /translation="EGRGTTRPFEIFGAPWIEEIPPGFYVCDLSYDGSVAAGNIVGDG
                     SYEMVAGGREGALNCYSGGIHSLASAPENHAKGKDSFHSFAWPNPSSEQVSITFFLPS
                     ENSVVVDIFTAGGKRIGTIHDQRMKAGEHTVTWDGKDLSGSVCTPGYYIYSIQTTKAR
                     SSGGIIRQ"
     gene            569..988
                     /locus_tag="PKZ74_02875"
     CDS             569..988
                     /locus_tag="PKZ74_02875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPM91726.1"
                     /translation="MKDLISTTEFSVRFSEVDSMQIVWHGHYIKYMEEGREDFGRKFG
                     INYMLIKANGFMAPLVSIKCEYKKTVSYDERVKVETRYIYSEAAKITYVFRMFRASDG
                     ELVATGESTQVFLDMNGELVLTNPPFFEEWKKRWGLK"
     gene            1021..2202
                     /locus_tag="PKZ74_02880"
     CDS             1021..2202
                     /locus_tag="PKZ74_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPM91727.1"
                     /translation="MRRVYFGADNIITSLGFSTEENIRNIGSGISGIRLHEDSAVSLH
                     PAAFSAVDPNALDEHFAAPDLNRRFTGSSSLPFSRLEKMLLISLSDVINTCGIDMRNP
                     RTLLIISSTKGNIDRLALSPVKTGGDLRLHLWKTAGFIAQLFNCYNLPIVVSNACTSG
                     SAAIITAARLIQAGKYDHVVVAGGDIVTEFVISGFQSFQALSMEACKPFDLNRTGLSL
                     GEGCGTLLLTCDPEVSGGASPVEYLGGATSNDANHISGPSRDGEGLYLAIKAAMEESG
                     ESAGDIEFISAHGTATPYNDEMESLALARAGLENVPVNSFKGYFGHTLGAAGIIETIL
                     TAYSIRNAILFKSTGYQNHGVSKPLNIISQNIKKPINTALKTASGFGGCNAAIILGNS
                     E"
     gene            2256..3446
                     /locus_tag="PKZ74_02885"
     CDS             2256..3446
                     /locus_tag="PKZ74_02885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM91728.1"
                     /translation="MDPVFITGMGVITSIGRNTEENLASLKAERTGIRMLSTIESVYR
                     GILPAGEILFSDKELASLAEMKYADGLSRTSLLGMIAAREARVNANCMASDGNRTGFI
                     SGSTVGGMAATEKFYPDYLTNDSRNAWIDTNESRESTERIADLLGIRDFVSTINTACS
                     SSANAIMTGARMIRAGMLDRVIAGGVDSLSMFTLNGFNTLMIYDKAPCRPFDDNRNGL
                     NLGEGAAYLVLESEKSLAGRRPLCRLTGWGNSTDAHHQTALSPEGTGPFLAMQAALAV
                     GGLNPSDISYLNVHGTATVNNDLAEGIGMRRLFGEDWPLFSSTKPFTGHLLGASGSVE
                     SVYSILSILNDTAWANLNFSTPMKELDKSPVARTRSGAGIRHVMTNSFGFGGNNTSLI
                     FSVL"
     gene            3448..4521
                     /locus_tag="PKZ74_02890"
     CDS             3448..4521
                     /locus_tag="PKZ74_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HPM91729.1"
                     /translation="MKLFINGTGNVSAQRGWDSISDPGGPAGYSGRMIRCPEPEADTG
                     LDDKYLRRMSRMIRLGWIAARLCLDDAGNPVPDAIITGSGWGSVLDSEKFLLSIYKNN
                     ENLLPPTPFIQSTHNAVGAQVAMLLGNCSYNMTYAHGIFAFEHAMLDAMLCFMEGGKN
                     NILLGGFDEITDNQFILTDRLGRWRKEPVQSLEMIDRKSKGTVAGEGYSFFFLQDHPS
                     STTYARLTGVRTIYRTDNKDDVSVAIIKFLNDHGLKPGDISCIFAGYNGDEQGDLYYD
                     DVLARVFRKGVPEARWKHLTGDYLTAGAFATWAACKSIKAQTIPASLLSSGHPQAPIR
                     YILIYNHFNCAHHSLILISHPDE"
     gene            4530..5630
                     /locus_tag="PKZ74_02895"
     CDS             4530..5630
                     /locus_tag="PKZ74_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM91730.1"
                     /translation="MNKRLYLPKFPHQFTFNLGLRIRLMLTRLTDRLVPAPMAVYEKA
                     QGIWISGAIGTACELNIADHLAPGPKSIMELARKTNADEDFLYRLMRALAGEGIFKEM
                     PGKVFANNRLSLPLADGEKSMKYMIRHQFGETNMTLFLHLTESIREGQGNSQKFLGKR
                     VFQYLQDHPLNNDIYNQAMNSSSNLVAVALLSAYRFKGIKTLVDVGGGQGVLLKLIIE
                     KHKHIRGILYDQKHVTQKADMVLKESSDKGKIEIVSGNFFTDVPAGADGYFMKNILHA
                     FHDTDCIRLLKKINEVMPDHGKVIILENVTFPDNKPSFGKRLDLMMMTGTDGGKERTR
                     EEFESLLDRSGFRLKRFIRTISPFFVLEAIKK"
     gene            5743..6129
                     /locus_tag="PKZ74_02900"
     CDS             5743..6129
                     /locus_tag="PKZ74_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91731.1"
                     /translation="MKEKDLYIESKEQQMILYAEKEDESYGEIATGSYATKHHLPEFI
                     KYKETLDKELRKELMAGKISPLYYFMLMQDMGPGDLAKRVGVNRWKFKKQMKPENFDK
                     LGDKILEKYALVFGVSIERIKELKTH"
     gene            6130..7140
                     /locus_tag="PKZ74_02905"
     CDS             6130..7140
                     /locus_tag="PKZ74_02905"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPM91732.1"
                     /translation="MDTREIPFEHRMAAHCETGTVTALLNHGGLTLTEPLVFGIAGGI
                     FFGYLKSPDLPFPMFILRSKPGNIRRKISKRLGIKFAESRFKNPSEGEAALDKAIRDG
                     HPTAVQVDFFYMDYMPEWQRVHINAHYVIVCGVNGSHYTVSDSYYKQKSLLERQKMLT
                     ARFAGGMMAPKGMMFYTDHIPEKIDLTKPIITGIKRASFNMTRLPIPFLGIKGIHRFA
                     EKVKEWPALTRDTDELADRIMRINILLEDQGTGGGGFRYMYATFLQQAAPIVNDDRLK
                     SFSKEMMEIGDKWRNISYFAAKISKNRDLGPERFEELSRLIHAQGDEEQKFFSELYKL
                     VK"
     gene            7153..7431
                     /locus_tag="PKZ74_02910"
     CDS             7153..7431
                     /locus_tag="PKZ74_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM91733.1"
                     /translation="MTAEETTLLKTEIKELIIQTLNIKNIKAEQVPDAVPLFSPENIL
                     ALDSIDGIELVMAIQEKYQIRIADQNVARNVLESVDSIAAFVIREKGL"
     gene            7441..8520
                     /locus_tag="PKZ74_02915"
     CDS             7441..8520
                     /locus_tag="PKZ74_02915"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM91734.1"
                     /translation="MHSYILTGIRIGPLMRLLRDNGFTPSPKNIGRLIFVFQNGLWSS
                     ILARREKKLHGRNISQWPVPDDPLIIVGHWRTGSTFLHQLLSCDDRFISPTLYQCSFP
                     ESFINGERFYRPVMGAMVRKRPIDNMDMGFDDAQEDEFAWVKLAPGSPFLKFVFPDGQ
                     GYFVNAADDFIPPPPVRRIWKEQMQQFGAKICRDTGKRLLLKNPFHSLRIPFIREVFP
                     NAKFIHLHRHPYEVVASSLSLWKVVARDNQLKGKPGIPGTEEVAEGLQKFYSVIKRDL
                     AGLPENNYFEISYESLVKDPILELNRLYVRLGMDFSDAARENMAARLQKVSNFRKNTY
                     TFDDSQKELVYRVLRKEFEQYHYPV"
     gene            8522..9787
                     /locus_tag="PKZ74_02920"
     CDS             8522..9787
                     /locus_tag="PKZ74_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HPM91735.1"
                     /translation="MILRLIYPRWTRLEGQNTFILPPLGPTAFAAALPGDITIDFIDE
                     NLEPVNFDDPCDLVGISMMLTAQVKRGWEIADEYRGRGKKVIFGGISTMLHAQETIPH
                     ADSVFLGEVEGRMEEVIGDFLRGELKQVYNYLPHPPDTALIGPARRDILHEDLYYHKG
                     IRMVDLFHASRGCRFACYPCAASFLGGRRFRPRPFDKVAEELDAIKNNRLFIVDNSLA
                     QDKEWEKELFRMMIPFKKNWCSHTIDDDPEILDLAAQAGAWYVYQAVIDDSDYIRKRI
                     QRYHDFGIAVEATVLLGMDDQTEDDILRLIDFLQEVNLDLAEFTVLTPFRHTKVWDDM
                     ERDGRIIDRDWSNYNAGKVVFKPRHMSPDRLQELYHYAWEKFYEHENQEQKMFKMIMK
                     VIERERKQGTYQPPRKDLARMSFGQIIDR"
     gene            9791..10144
                     /locus_tag="PKZ74_02925"
     CDS             9791..10144
                     /locus_tag="PKZ74_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91736.1"
                     /translation="MDNQDKYTREVFYFSGQWEMPSHCGILKEERDGMTYVILTEMYE
                     TNPGSSVTSMIETIAKDILSIYGMDPGKAEFIVRTPMRNAHYTFYAETFYRAMMKWDG
                     ERFTGLEWEKMEDFR"
     gene            10146..12521
                     /locus_tag="PKZ74_02930"
     CDS             10146..12521
                     /locus_tag="PKZ74_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91737.1"
                     /translation="MRCHLKIGSFLLPWLISVNSLPLFSQQFPLQTVRGIILDKTSQS
                     PLPGANITILTTDPVMGASSDAGGQFIIRNVPTGRHRIQASFIGYLTITLDNQMVTSG
                     KELVLKLEVEESAVEGKEVEIIGRLRKDQPVNPMAMISARSFTIEETSRYAGSYGDPA
                     RMAVNYAGVVSTRDNRNDIIVRGNSPFSLKYRIDGIEILNPNHFGATGTTGGPVTILN
                     TNMLANSDFLTGAFPAEYGNATAGIFDIHLRNGNTGKREYWGQLGFNGLEFGTEGPFK
                     TGGASSYLASYRYSFTGLAERAGIRLEESADYQDMGLRFSFPTKKAGIFRFTALGGIS
                     AIRLMDSEQERNEWIFDSHGEDLRTGSHLAAAGLSHLFFINEDARIQSYLSFTSSGIE
                     TTIDTFELGSPKPFRWAGERSRENKISASVHYKYKINSQNNLNAGVYYDLFMMNYLDS
                     QFIKQEYIYHTDAKENLGLLRAYGGYQHRFGPAFAASLGLHLQHLSMNGSFSAEPRFG
                     IRWDVTPLSAISGGFGLHSQMVPSMLYFTQSRLPDGTYIQTNRDLDFMRSLHAVAGYD
                     YLIREHLRLKAEVFYQELYKIPVKESIPQYSVINEGLDYFILRYDSLVSRGSGTNFGL
                     DLTMERFLHRNYYFLFTASLYRSDYTGYDNVKRNTAFDNRYVVNAVGGYELPFGKMKN
                     QFLVLGLRATWTGGRPYLPFDTERTTATGQVEYDWQNAFGVRFEEYIRSSLRIGYRRN
                     VTHANIMLLLDLQYRSNYTNIDLYRIDVKTGEIVQGYRMGFYPMATWRIQF"
     gene            12553..14064
                     /locus_tag="PKZ74_02935"
     CDS             12553..14064
                     /locus_tag="PKZ74_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM91738.1"
                     /translation="MKIAIIGAGISGLSAGCYLQMHGFDTEIYEKHSRAGGLCTTWKK
                     GDYSFDGCIHWLLGSGERNPLYSLWSELIEMDSVKFVHHDTRFHIELKDNTDKYGSKV
                     FHLYNNIDRLEKYLLDLAPEDRVPIRKFIRMMRRIQGWEVPPEIKTPPSLFTFRQKIG
                     MARHLPLLLFMLKNRNLTNYSFAGRLKNPFLKEAFQLLFDGEEVSLLVIAFPLAFSDL
                     KATGYPIGGSAKFAERISERYISLGGRINYLSGVKEITVKDDAATGLLLEDGREIRSD
                     LVVSAADWRFTVFEALKGRYVNQKLLDLRDGKKFRVYYSVINVSLGIGRPMGDVPQLT
                     RFPLEKPLISPDGTVFERLELHTYHYDPTLAPAGKTSASASFYTSNGEFWIDLYREDR
                     ERYDQVRKAFAAEVIDILDKKFGRIKEYLEVTDVATPATYHLFTGNWRGSVQGWLPGK
                     NIMAPSPVTMKLPGLKNFYYASHWSVPGGGLPTALKSARDMAFTIAAEYRKKR"
     gene            14178..>17521
                     /locus_tag="PKZ74_02940"
     CDS             14178..>17521
                     /locus_tag="PKZ74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPM91739.1"
                     /translation="MRAFRCLVLIIAFFLAQKGFPQQTLWVTHWADSGPGSLREVISS
                     ASAGDIIRFVPNISSITLSSAEIHINKPIKIIGNEDIITIKRNLSESTPHFRIFNISY
                     TDYPLDTLILKNLNFKYGFSSDGDDENQDGKDGGAIIASNPNNIIKIINCQFTNNQCG
                     NGITNLHVTTKSKKRADSGRGGHGGAIYSDAMLIIENCSFSLNETGSGGAIYLNNINW
                     FIEAGIGGHGGAIACNSYLIINDCLFNNNHTKSGGLAKSSSRYTSMALGGSAGYGGAI
                     YCSSGVEIINTTFTGNSTGTGGTGIGDGWVMGVGGDGGHGGALFAIDSTACVTNCTFS
                     ENKAGDGGLEGGDGAAHGGSGGYGGCIYTKNAWIIIDNCSFLNNTAGAGTSGSAYHAG
                     LGGDGGSGGAICAIDSQIEILESVFTDHCAGNGGYGGSAVWSIAGKGGHAGAVYVENS
                     YGAIKSTIFQRNHTGKAGDSYVQHEKIDTNISINGGSGGAVFLSGQSSLLIMKSEFSA
                     NNTLDGGSIIGPGDSLTPGGDGGNGGAIALSQNSLLRMVNSLVYGNSTGIAGVNITDS
                     TMTGSNGTGGGISLNENSNLMLINSIIAGNLTGVTEKPGSFIPALGLGGGIYCEDTSI
                     SIFNSIIAFNKVNKLNSICDNELFTNSHLDHCMIYDTSGFSYTGTGNIFHKDPQFLNF
                     PDNLNVLPSSPAINNGNPDTTGLSLPEYDLIGNQRIYDRIDIGAFEYPYELSDSLCII
                     SDAIDFNDVIIGLSVIDSLKLVNVGDATILIDSLLMPDDFSILADDAIWSNRLDSIIL
                     YSGDEEQYFVRFMPEYPGRYMDSASIFINLHPDPHILKVMGEGIQPLEIDISSNCSDS
                     VFAGQLYSCRIEIESHNGFPVDVQIFEKPNWCNLNNQSDTLLEISGAPVYSDIGQHEL
                     HIRVGDGLIFNEFYQEIDVIEPIIPYVEYNCPLSINVEQIFNCLISALGNNHLPVNIE
                     IINYPNWIDWNQKSDDTIEVYGTPGINDIGSALFEFSAFDSLAEHTISFTVVIGPTGT
                     LSPDDIIYFQRFIISPNPSKDLFYLAFHDDNLPNELEIGIYSCTGTRIDHLFINETCN
                     TVPINLQNLPAGIYFVRLTIDGKETVT"
BASE COUNT         4602 a         4173 c         4533 g         4213 t
ORIGIN      
        1 cgagggtagg ggaaccactc gcccgttcga gatattcggc gcgccgtgga tcgaagagat
       61 cccgccgggg ttctacgtgt gcgatctctc ctatgacggt tcggtcgcgg cgggcaacat
      121 tgtcggcgac ggctcgtatg agatggtggc cggaggaagg gaaggcgcac tgaattgtta
      181 ttcgggaggc atccacagcc tggcttccgc ccctgaaaat catgcgaaag ggaaggattc
      241 ctttcattcc tttgcctggc caaacccctc gtctgaacaa gtcagcatca ccttttttct
      301 tccttcggaa aacagcgttg ttgtcgatat cttcacggcg ggcggaaaaa ggattggaac
      361 catccatgac cagcgcatga aggcaggcga acatactgtt acctgggatg gaaaagatct
      421 ttccggctcc gtttgcacac caggatacta tatttatagc attcaaacga cgaaagcccg
      481 gtcatcggga gggataatca ggcaataaca agggataaag gtaaagggaa aagcgaaaag
      541 cgaaaagcga aaagatgatt ttatactaat gaaggattta atatctacga cagaattttc
      601 ggtgcgtttc agcgaagtcg actcgatgca aattgtctgg cacggccatt acattaaata
      661 catggaagag gggcgggagg atttcggaag aaaattcggc atcaactata tgctgatcaa
      721 ggcgaacggg tttatggccc cgctggtcag catcaaatgc gaatacaaaa aaaccgtgtc
      781 gtacgacgaa cgggtgaaag ttgagacccg atacatttat tccgaagcgg ccaaaatcac
      841 ttatgttttc cggatgttcc gggcgtctga cggggagctg gtggcaaccg gtgaaagcac
      901 tcaggtattc ctggatatga acggcgagct ggtattgact aaccctccgt ttttcgaaga
      961 atggaaaaaa agatggggct taaagtgaga ttttctattt ttggcgggta atacagattt
     1021 atgcgaaggg tttacttcgg agctgacaat atcattacct ccctgggatt tagtacggaa
     1081 gaaaatatcc ggaatatcgg atccggtatt tcaggtatcc gcttacatga ggattcggcc
     1141 gtttcgcttc accccgctgc tttttcagcg gttgatccga atgcgcttga tgagcatttc
     1201 gctgctccgg atttgaatcg tcgtttcaca gggagttctt cgttgccatt ttcgcggctt
     1261 gaaaagatgt tattaatctc gctatcggac gtaatcaaca cgtgtggtat tgacatgcgt
     1321 aacccccgaa ctcttctgat catttcctct acgaaaggaa acattgaccg gctggcgcta
     1381 tcgccggtta agaccggggg ggatttaaga ttgcaccttt ggaaaacagc cggatttatt
     1441 gctcaattgt tcaattgtta taatcttccg atagtggtca gcaatgcctg cacatcgggt
     1501 tcggcggcca tcatcacggc agcacgactc attcaggctg gaaaatatga tcatgttgtg
     1561 gtagctggcg gcgatatagt caccgagttt gtgatttcgg gatttcagtc gttccaggcc
     1621 ctcagcatgg aagcctgcaa acccttcgat ctcaaccgga cgggattgtc gctcggggaa
     1681 gggtgcggca cgctgctcct gacctgcgat ccggaggttt caggcggtgc atcaccggtg
     1741 gaatacctgg gcggggctac gagcaacgat gccaaccata tttcaggtcc ttcaagggat
     1801 ggcgaagggc tttacctggc catcaaagcc gcgatggaag aatcggggga aagtgccggc
     1861 gacattgagt tcatttcggc tcatggcacg gcaacgcctt acaacgacga gatggagtcg
     1921 ctggcgctgg cccgggccgg attggaaaat gttccggtaa acagctttaa aggatatttc
     1981 ggacacaccc tgggggctgc cgggatcatc gaaacgatcc tgacggcata ttccatccgc
     2041 aacgctatcc tttttaaatc aacgggttat caaaatcacg gtgtttcgaa acccttgaat
     2101 attatctcac aaaatatcaa aaaaccaatc aacacggcgc taaaaaccgc ctccggcttc
     2161 ggagggtgca atgcggcgat cattttaggg aatagtgaat agtggtcaga tacatggaaa
     2221 atggacaatg gacaatggac aatgaatagc ttctgatgga tcctgttttt atcaccggta
     2281 tgggggttat cacttcgatt ggaaggaaca cggaggagaa cctggcttcg ctgaaggcgg
     2341 aaagaacggg tatccggatg ctttcaacga tcgaatcggt ttaccggggc attttgcctg
     2401 ccggggagat ccttttcagc gataaagagc tggcctcatt agcggagatg aaatacgcgg
     2461 atggattatc caggacatcg cttctgggga tgattgcggc ccgggaagca cgggtaaatg
     2521 caaactgcat ggcgagtgat ggcaacagga ccggttttat ctccggttcg acggtcggcg
     2581 gaatggcggc cactgaaaaa ttttatcccg attacctgac gaatgacagc cggaatgcct
     2641 ggatcgacac caacgagagc cgggaaagca cggagcggat cgccgacctg ctgggtatcc
     2701 gcgattttgt ttcgaccatc aacaccgcgt gttcctcctc tgccaatgcg atcatgaccg
     2761 gcgcaaggat gatccgggcc ggtatgctcg accgggtcat cgccgggggg gttgattcgt
     2821 tgtcgatgtt caccctgaac gggtttaaca cgttgatgat atacgacaaa gcgccatgcc
     2881 ggccgttcga cgacaaccgg aacggtctga acctgggcga aggggctgct tatcttgtgc
     2941 tggaatcgga gaagagtctc gcaggacggc gtccgttatg ccggctaacc ggatggggca
     3001 actcgaccga cgcacaccac cagacggcgt tgtcgcccga agggacgggc ccctttctgg
     3061 ccatgcaggc tgcactggcc gtcggtgggt tgaatccgtc tgatatcagt tatcttaacg
     3121 tacacggtac tgctaccgtc aacaatgacc tggcggaggg gatcgggatg agaaggttat
     3181 tcggtgagga ctggccgctt ttcagctcaa caaagccatt tacaggacat ttgctgggcg
     3241 cttcgggatc ggtggaatcg gtttactcga tcctgagcat cctgaacgat accgcgtggg
     3301 cgaacctgaa cttttccaca cccatgaaag aacttgataa aagtcctgtt gccaggaccc
     3361 ggtccggtgc cggtatcagg catgtcatga ccaattcgtt cggctttggc gggaacaaca
     3421 catcgcttat tttttctgta ctctgatatg aaactattca tcaacggcac cggcaatgtc
     3481 tcagcgcaga gaggctggga ctccatctcc gatccgggag ggccggccgg ttattcgggc
     3541 cggatgatcc ggtgcccgga gccggaagcc gatacagggc tcgatgataa gtatcttcgc
     3601 aggatgagcc ggatgatccg gctggggtgg atcgccgcca ggttgtgcct ggacgatgcc
     3661 ggcaatcctg ttcccgacgc tatcattacc ggctcgggct ggggcagcgt cctcgactcc
     3721 gagaaatttc ttctgtccat ttataaaaac aacgaaaacc tcctcccgcc gacgcctttc
     3781 atccaatcaa cccacaatgc cgtaggggcg caggttgcca tgctccttgg caactgttcc
     3841 tacaacatga cctacgcgca cggcatcttt gctttcgaac acgccatgct cgatgcgatg
     3901 ctttgcttta tggaaggcgg gaagaataat atcctcctcg gcggcttcga tgagatcacc
     3961 gataaccagt tcatcctgac cgacaggctg gggcgctggc ggaaagaacc cgtgcaaagc
     4021 ctggaaatga tcgaccggaa gagtaaagga acggttgctg gcgagggtta ttccttcttc
     4081 ttcctgcagg atcatccatc gtcaacgacc tatgcccggc tcaccggggt aaggacgata
     4141 taccgaacgg ataataaaga tgatgtgtcg gtcgccatta taaagttcct taatgaccac
     4201 ggtctgaaac ccggagatat cagttgtatt tttgccggct acaacgggga tgaacaggga
     4261 gatctatatt acgatgacgt cctggccaga gtcttccgaa agggagtgcc ggaagcccgg
     4321 tggaagcacc tgacgggtga ttatctgacg gccggcgctt ttgctacctg ggcggcctgc
     4381 aagtcgatca aagcacaaac gattccggca tcattgttaa gttccggcca tccccaggct
     4441 cccatcagat atattttgat ctataatcat ttcaattgcg cacatcacag cctgatattg
     4501 atttcgcatc ctgatgaata aaggaaatta tgaataagag actatatctg cccaaatttc
     4561 cccaccagtt tacttttaac ctgggattac gcatcaggct gatgctgaca aggttaaccg
     4621 acaggctggt gcccgctccc atggctgttt atgaaaaggc gcaggggata tggatttcgg
     4681 gcgccattgg aacggcttgc gaactgaata tcgccgacca cctggccccg gggcctaaaa
     4741 gcatcatgga gctggcccga aaaaccaatg cagacgaaga ttttctttac cggttgatgc
     4801 gggcgctggc cggtgaaggc atatttaaag aaatgccggg aaaagtattt gcgaataacc
     4861 gcctctcttt accacttgcc gatggtgaga aatccatgaa atatatgatc aggcaccagt
     4921 ttggcgaaac caacatgacc ttgttccttc atctgacaga atctatccgg gagggacagg
     4981 gaaactcaca gaagttcctt ggaaagaggg tattccagta tcttcaggat catcctttaa
     5041 acaatgacat ttacaaccag gctatgaaca gttcctctaa cctggtagca gttgcccttc
     5101 tgtcggccta ccggttcaag gggatcaaaa cactggtgga tgtaggtggc ggccagggag
     5161 tccttctgaa actgatcatc gaaaaacaca aacatatccg gggaatcctt tacgaccaga
     5221 agcacgttac ccagaaagcg gacatggtgc tgaaggaatc ttctgacaaa gggaagatag
     5281 aaattgtcag cggaaatttt ttcactgacg tgccggccgg ggccgacggt tatttcatga
     5341 aaaacatcct gcatgctttc catgataccg attgcatcag gctgctgaaa aagatcaacg
     5401 aagtaatgcc tgaccacgga aaagtcatta tcctggaaaa tgtgacattt cctgacaaca
     5461 aaccctcctt cgggaagcgg cttgacctca tgatgatgac cggaacagat gggggaaaag
     5521 agcggacccg ggaagaattc gaatccctcc tggaccggtc cggattccgc ctgaaacggt
     5581 tcatccggac gatttcgcct tttttcgtcc tggaagccat caaaaaataa tttcattttc
     5641 atcttcttat cataaaaaaa aactcgctta tttttgtcgt caggataaat gatccggata
     5701 ttccacccgg taaaaatctc cctcaaatga aaaaacgcta agatgaaaga gaaggatctc
     5761 tatatcgaat ccaaagagca gcagatgatc ctttatgccg aaaaggagga tgaatcctac
     5821 ggtgaaatag ccaccggatc ctatgctaca aagcaccatc ttcctgaatt tataaagtat
     5881 aaagaaacac tcgacaagga attgcgtaaa gaattaatgg ccgggaagat cagccctttg
     5941 tattatttta tgctgatgca ggatatgggg cccggggatt tggccaagcg agtcggggtt
     6001 aaccgatgga aatttaagaa acagatgaaa ccggagaatt tcgacaaact cggcgataag
     6061 attctggaaa aatacgctct cgttttcggc gtgagcattg agcggatcaa ggaactcaaa
     6121 acacactgaa tggacacacg ggagatacct tttgagcaca ggatggcggc tcattgtgag
     6181 acgggaactg ttacggcatt attaaatcac ggcgggctta cgcttacaga gcccctggta
     6241 ttcggaatcg ccggcggcat ttttttcggc tacctcaaat cgcccgacct gccctttccc
     6301 atgtttattt tgcgaagcaa gccgggaaat atccgaagaa aaatttcaaa acgattaggg
     6361 ataaaatttg ccgagagcag gttcaagaat ccgtctgaag gagaagcggc gctggataag
     6421 gccatccgcg acgggcaccc gaccgcggtc caggtcgatt tcttctatat ggactacatg
     6481 ccggaatggc aaagggttca tatcaacgcg cactatgtca ttgtttgcgg ggtgaacggc
     6541 agtcattaca ccgtcagcga cagttattac aagcaaaaat cgctgcttga aaggcagaaa
     6601 atgctgacag cccgttttgc cggcggaatg atggcgccta aaggcatgat gttttatacc
     6661 gaccacattc ctgaaaaaat cgatctgacg aaacccatca tcaccgggat aaaaagagct
     6721 tcattcaaca tgacccggct gcctatcccc ttcctgggga taaaaggcat tcacaggttc
     6781 gccgaaaagg taaaagagtg gccggcattg acccgtgaca ccgatgaact ggctgaccgg
     6841 atcatgcgga tcaatatcct actggaggac caggggacgg gcggcggcgg cttccgctac
     6901 atgtacgcca ctttcctgca gcaagccgcc cccatcgtca atgacgaccg gttgaaatcg
     6961 ttttccaaag aaatgatgga aattggcgat aagtggagga atatttctta ctttgcagcg
     7021 aaaatttcaa agaaccgcga cctgggaccg gaacgtttcg aggaactgag ccggctgatc
     7081 catgcccagg gcgatgaaga acagaaattt ttcagcgagt tgtataaact tgtcaaataa
     7141 acctaataaa ggatgacggc ggaagaaacc acattgctga agaccgagat caaagagctc
     7201 atcattcaga ctctcaatat aaaaaatatc aaagcggaac aggtacccga cgctgttccc
     7261 ctgttttctc cggaaaatat actggcactc gattccatcg acggcatcga actggtgatg
     7321 gctatccagg aaaagtacca gatccggatc gccgatcaga atgtggccag gaatgtactt
     7381 gagtcggtcg attccattgc tgcattcgtc atcagggaaa aagggctctg atcctgcctc
     7441 atgcattcct acatcctcac cggaatccgt atcggacccc tgatgcgtct tttgcgtgac
     7501 aacggtttta ccccctctcc caaaaatatc ggacggctga tctttgtttt ccagaatggc
     7561 ctctggtcgt cgatcttggc acgcagagaa aagaaactgc atggccggaa cataagccag
     7621 tggccggttc ccgacgaccc gctgatcatc gtcggacact ggcggaccgg cagcactttc
     7681 ctgcaccagt tgctgtcgtg cgatgatcgg ttcatctccc ctacccttta ccagtgctcc
     7741 ttcccggaat ctttcatcaa tggtgaacgt ttttaccggc cggtgatggg agcaatggtc
     7801 agaaaaagac cgatcgacaa tatggatatg ggattcgatg atgcccagga agatgagttt
     7861 gcctgggtca aattagcgcc gggctcacca tttctgaaat tcgtgttccc ggatggccaa
     7921 ggttattttg tcaatgcagc cgatgacttc ataccgccgc ctcctgtgcg tcgtatctgg
     7981 aaggaacaaa tgcaacagtt cggggcaaag atatgccgcg acaccggcaa gaggctgctg
     8041 ctgaaaaacc cgttccattc cctgcgaatc ccttttatcc gggaggtttt cccgaatgca
     8101 aagtttatcc atcttcaccg gcatccctac gaggtggtgg cttcttcctt aagtctctgg
     8161 aaggtggtgg cccgtgacaa ccagttgaaa ggcaaacctg gtatccccgg cacggaagag
     8221 gtagccgaag ggctgcagaa gttttacagt gtgattaaaa gggacctggc cgggttaccg
     8281 gaaaataatt attttgagat tagttatgaa tcgcttgtga aagatcccat tttggagtta
     8341 aatagacttt atgtaagatt aggaatggac ttttcggatg cagccaggga aaatatggcg
     8401 gcgcgtctgc aaaaagtcag caatttcaga aaaaatacct atacatttga cgacagccag
     8461 aaggagctgg tttaccgggt cctgagaaaa gagtttgaac aatatcatta ccccgtttaa
     8521 tatgatcctc cgtttgatat atccccgctg gacccggcta gaagggcaga acacctttat
     8581 actaccgccc ctggggccca cagcatttgc cgccgcctta cccggcgata tcaccatcga
     8641 tttcatcgat gaaaacctcg agccggtcaa ctttgatgat ccctgcgacc tggtgggcat
     8701 ttcgatgatg ctgactgccc aggtcaagcg cgggtgggag attgccgacg agtaccgcgg
     8761 ccggggaaag aaggtcattt tcgggggtat ttcgacgatg ctgcacgcgc aggaaacgat
     8821 cccccatgcc gacagcgtct tcctgggcga ggtggagggc cggatggaag aggtgatcgg
     8881 cgatttcctc cgcggagagc tgaagcaggt ctataactat ctaccccatc cgcccgatac
     8941 ggctttgatc gggccggccc ggcgcgacat cctccatgaa gacctctatt accacaaagg
     9001 catccgcatg gtcgatctgt tccacgcatc ccgcggttgc cgctttgcct gctatccctg
     9061 cgccgcttca ttccttggag gacggcggtt caggccccgg ccgttcgaca aggtggccga
     9121 agaactcgac gcgatcaaaa acaaccggct gtttatcgtc gataattcgc tggcacaaga
     9181 taaagagtgg gaaaaggaac ttttccggat gatgatccct ttcaaaaaga actggtgcag
     9241 ccacaccatc gacgacgatc cggaaattct cgacctggcg gcacaggccg gcgcctggta
     9301 tgtgtaccag gctgtgatcg acgattccga ctacatccgg aagcggatcc aacggtacca
     9361 cgatttcggc attgccgtgg aagcgaccgt gctcctgggg atggatgacc agaccgaaga
     9421 cgatatcctc cgcctgatcg acttcctgca ggaggtaaac ctcgacctgg ccgaattcac
     9481 ggtgcttacg cctttcaggc ataccaaagt gtgggacgat atggagcgcg acggccggat
     9541 catcgaccgc gactggtcga actacaacgc cgggaaggtc gttttcaagc cgcgccatat
     9601 gtcgcccgac cggttgcagg aattgtatca ttacgcctgg gaaaaatttt atgaacacga
     9661 gaaccaggaa cagaaaatgt tcaagatgat catgaaggtc attgaacggg agcggaaaca
     9721 aggcacctac cagcctcccc ggaaggacct ggccaggatg agtttcggac aaattatcga
     9781 ccgctgaaat atggataatc aggataaata cacccgggag gtcttctatt tttcaggaca
     9841 gtgggaaatg ccatcccact gcggaatcct gaaggaggaa agagatggga tgacttatgt
     9901 tatccttacg gagatgtacg agacaaatcc cggaagctcc gtcaccagca tgatcgaaac
     9961 catcgcaaaa gatatcctca gtatttacgg gatggatcct ggaaaagccg aattcatcgt
    10021 ccgtaccccc atgcggaatg cccattacac attttatgca gagacttttt acagggcaat
    10081 gatgaaatgg gacggcgaaa gatttaccgg cctggagtgg gaaaagatgg aggatttccg
    10141 gtaatatgag atgccatctc aaaataggtt ctttccttct cccctggctg atttccgtaa
    10201 actctttacc tctgttttca cagcaattcc ccttgcaaac cgtcagggga attatccttg
    10261 ataaaacgtc gcaatcgccc ctgccggggg ccaacattac catcctgacc accgacccgg
    10321 tcatgggcgc ttcttccgac gcgggaggac agtttatcat ccggaacgtg cccacaggcc
    10381 ggcaccggat ccaggccagt ttcatcggat atctgaccat aacactggat aaccagatgg
    10441 tgacctcggg caaagagctg gttttgaagc tggaggtaga agagagcgcg gtggagggca
    10501 aagaggtgga gatcatcggc cggctgcgca aagaccagcc ggtgaacccg atggcgatga
    10561 tcagcgcccg gtcgttcacc atcgaagaga ccagccgata tgccggcagc tacggcgacc
    10621 cggccaggat ggccgtgaat tatgccggcg tggtgagcac acgcgacaac cgcaacgata
    10681 tcatcgtccg cggcaactcc ccgttcagcc tgaaatacag gatcgacggg atcgagatcc
    10741 tcaacccgaa ccacttcggg gccacaggca ccaccggcgg gccggtcacc atcctgaaca
    10801 caaacatgct ggccaattcc gactttctga cgggcgcctt cccggccgaa tacggcaatg
    10861 ccaccgccgg cattttcgac atccatctcc ggaacgggaa taccggcaag cgggaatact
    10921 ggggccagct tggtttcaac gggctcgaat tcggtaccga agggccgttc aaaacaggcg
    10981 gcgcctcctc ctacctggcc tcctaccggt attcgttcac cgggctggca gaaagggccg
    11041 gcatccgcct cgaggagagc gccgattacc aggacatggg gctgcggttc agcttcccga
    11101 ccaaaaaagc gggtattttc cgttttaccg ccctgggcgg gatctcggcc atccggctga
    11161 tggactcgga gcaggagagg aacgaatgga tattcgattc acacggagaa gacctcagga
    11221 ccggttccca cctggccgcg gccggactca gccatctttt ttttattaac gaagacgcac
    11281 gcattcaaag ctatctttcc ttcacctcca gcggtatcga aaccacaata gacactttcg
    11341 aactgggcag cccgaagcct ttccgctggg ctggcgaaag gtcgcgcgaa aataaaatct
    11401 cggcatccgt tcattacaaa tacaagatca attcgcaaaa caacctcaat gccggtgtct
    11461 attacgatct cttcatgatg aattaccttg acagtcaatt cattaaacag gaatacatct
    11521 accataccga cgcaaaggag aaccttgggc ttctaagggc ttacggtggc taccagcacc
    11581 gcttcgggcc ggcttttgcc gcttcactgg gcctgcatct ccagcatctg tcgatgaacg
    11641 gctccttctc cgccgaaccc cgctttggga tccggtggga cgtcaccccg ctgagcgcta
    11701 tcagcggcgg atttggcctg cacagccaga tggtgccgtc gatgctctat ttcacgcaga
    11761 gccggctgcc cgacggaacg tacatccaga ccaaccgcga tctcgatttc atgcggagcc
    11821 tgcatgcggt ggcgggttac gattacctca tccgggagca cctgcggctg aaagcggagg
    11881 ttttttacca ggagctgtac aaaattcccg tcaaggagag catcccgcaa tactcggtga
    11941 ttaacgaagg gctggattat tttatcctgc gttacgacag cctggtgagc cggggcagcg
    12001 gcaccaactt tggcctcgat cttacgatgg aacggttcct ccaccgcaat tactatttcc
    12061 tcttcaccgc atcactttac cggtcggatt acacgggata tgacaacgtc aagcgcaata
    12121 cggctttcga taaccgctat gttgtgaatg ccgtaggcgg gtacgagctc cctttcggga
    12181 agatgaagaa ccagttcctg gtcctgggcc tccgggccac gtggaccggc ggacggcctt
    12241 acctgccgtt cgataccgaa aggaccacgg cgaccgggca ggtagaatac gactggcaga
    12301 acgcgttcgg tgtccgtttt gaggaataca tccggagcag cctgcggatc ggataccgga
    12361 gaaatgtcac ccatgccaac atcatgctgc tgctcgacct gcagtaccgg agcaattaca
    12421 ccaacatcga cctgtaccgg atcgatgtga agaccggcga gatcgtgcag ggttacagga
    12481 tgggcttcta cccgatggcc acgtggagga tccagtttta gcgggtgcgg gagtttttaa
    12541 tacctttgga agatgaagat cgcgataatc ggagcaggca tttcaggttt gtcggccggg
    12601 tgttacctgc agatgcacgg atttgacacg gagatctacg agaagcattc ccgtgcggga
    12661 ggattatgca cgacctggaa aaaaggggat tacagtttcg acgggtgcat ccactggctg
    12721 ctgggctccg gtgagcgaaa cccgctgtac tctctctggt cggagttgat cgaaatggat
    12781 tctgtcaaat ttgtccacca cgacaccagg ttccacatcg agctgaagga caataccgac
    12841 aaatacggaa gcaaggtttt ccatctctat aacaacattg atcgtcttga aaaatacctg
    12901 cttgaccttg ctcctgaaga cagggttccg atcaggaaat tcatccggat gatgcggcgg
    12961 atccagggct gggaagtgcc tcctgaaatc aaaaccccgc cttctctatt cactttcagg
    13021 caaaagatcg ggatggcaag gcacctgccc ctgttgcttt tcatgctgaa aaaccggaac
    13081 ctcaccaatt actcctttgc cggaagattg aagaacccgt tcctgaaaga agcattccaa
    13141 ttgctgttcg acggcgaaga ggtttcattg ctggtcatcg ctttcccgct ggccttttcc
    13201 gacctgaagg ccaccggtta tcccatcggt gggtcggcga aatttgcgga gaggatttcc
    13261 gaaagatata tttcattggg tgggaggatc aattaccttt caggggttaa ggagattacg
    13321 gtgaaggatg atgctgctac gggcctgctg ctggaggatg gccgggaaat caggtcagac
    13381 ctggtggtgt cggcggccga ctggcgtttt acggtgtttg aggcgttgaa aggcagatac
    13441 gttaatcaga aactgctcga tttgcgtgac ggtaaaaagt tcagggttta ttattcggtg
    13501 atcaatgttt cgctggggat cggccggccg atgggcgatg taccgcagct gaccaggttt
    13561 ccgctggaga agccgttgat ttcgcccgac gggaccgttt tcgagcggct ggagctgcat
    13621 acctatcatt acgatccgac gctggcgcct gccggcaaga ccagcgcctc agccagtttt
    13681 tatacttcca acggcgaatt ctggatcgat ctgtaccggg aagaccggga gcgatatgat
    13741 caggtacgga aagcgtttgc cgcagaagtc attgacatcc tggacaaaaa atttggccgg
    13801 ataaaagaat atcttgaggt gaccgatgtg gctacgccgg caacttacca ccttttcacg
    13861 ggcaactgga gagggagtgt ccagggatgg cttccgggga agaacatcat ggctccatcg
    13921 ccggtcacca tgaagctgcc gggtttgaag aatttttact acgccagcca ctggtcggtg
    13981 ccgggcggcg ggctgcccac cgcgctgaag tcggccaggg atatggcttt tacgattgca
    14041 gcggagtaca ggaagaaaag ataaagggaa cattgtgaat ctataaaaaa atttttatat
    14101 ttggaaagcc aataaccatc acaaaaccaa taagcacttg aaatgacgtc gaccgggttt
    14161 gtttatacca aactgatatg agagcattca gatgtttagt attgattatt gcattttttc
    14221 tggcacaaaa aggttttcct caacaaacat tatgggtaac ccattgggct gactcagggc
    14281 caggttcact gcgagaagtt atctcaagtg cctcagccgg agatattatt cgatttgttc
    14341 caaacattag ttcaattaca ctgagctcgg ctgaaattca tataaataaa cccataaaaa
    14401 taattggtaa tgaagatatt attacaatta aaaggaatct ttccgagtcc actccacatt
    14461 tcagaatttt taacatctca tatactgatt atccattgga tactttaatt ttgaaaaacc
    14521 taaatttcaa atacggattc agttcagacg gagatgatga aaatcaggat ggaaaggatg
    14581 gaggcgccat aatagctagc aatcctaata atattataaa gataatcaac tgtcaattta
    14641 ctaataatca gtgcggtaac gggattacta atttgcatgt taccacaaaa tcgaagaaaa
    14701 gagctgattc aggcagggga gggcatggtg gagccattta ctctgatgca atgctaatca
    14761 ttgaaaattg cagtttttct ttgaatgaaa ccggttcagg cggagctatc tatttgaata
    14821 atattaattg gttcattgaa gcaggtatag gggggcatgg gggtgcaata gcttgcaaca
    14881 gttacctcat tatcaatgac tgtctgttta ataataacca taccaaatct ggcggccttg
    14941 caaaatcatc ttcgaggtat acttcaatgg cacttggagg ttcagcagga tatggaggag
    15001 caatttattg ctcctcagga gtagaaataa ttaatactac atttacgggt aattcaaccg
    15061 ggactggtgg cacaggtatc ggtgatggat gggttatggg agttggcggg gacggggggc
    15121 atggaggagc tttatttgca attgacagca ccgcatgtgt gaccaattgc actttctctg
    15181 aaaataaggc aggagatggc ggtcttgaag gcggggatgg agcagctcat ggagggtcag
    15241 gaggatatgg aggatgtatt tatacgaaaa atgcatggat cattattgac aattgttcgt
    15301 ttctgaataa taccgccggc gcaggaacca gtgggagtgc atatcatgcg gggcttggag
    15361 gtgatggtgg aagtggagga gcaatatgcg ctatagattc tcaaattgag atccttgagt
    15421 ctgtctttac agatcattgt gcggggaacg gtggatatgg aggaagtgca gtatggtcga
    15481 tagccgggaa aggaggccat gctggagccg tatatgtcga aaattcttat ggagctataa
    15541 aatcaacaat atttcaacga aatcatacag gaaaagcagg agattcatat gtccaacatg
    15601 aaaaaataga tacaaatatt tcaataaatg gtggatccgg tggcgctgtt tttttatctg
    15661 gacaatcttc gttgcttatt atgaagagtg aattttcagc taacaatact cttgacgggg
    15721 gtagtattat cggtcctggc gactcactaa ctcccggagg agatggaggt aatggtggtg
    15781 cgattgcttt atctcaaaat tcattgcttc gaatggttaa cagtttagtt tatggaaatt
    15841 ctaccgggat tgccggggta aatatcactg acagcaccat gactggctca aatggaacag
    15901 gaggtggtat cagtctgaat gaaaactcaa acttgatgtt gatcaactcg atcattgcag
    15961 gtaatctgac gggggtgaca gaaaaacctg gctcctttat tccagctctc ggactaggag
    16021 gtggtattta ttgtgaggac acatccatct caatatttaa ttccattatt gcctttaaca
    16081 aagtaaataa acttaatagt atatgcgata atgaattatt taccaattca catcttgatc
    16141 actgtatgat ttatgacact tcaggatttt cctatactgg gacaggaaac atcttccata
    16201 aagatccaca attcttgaac tttcccgata atctcaatgt acttccttca agccctgcca
    16261 taaataatgg gaatccggat accaccggat tatcattgcc agagtatgat ttgattggca
    16321 atcaaaggat ctatgacagg atcgatattg gtgcttttga atatccttat gagctatcag
    16381 acagcctttg tattatttct gatgcgattg acttcaatga tgtaataata ggtctttcgg
    16441 taatcgactc attgaagttg gtcaacgtag gagacgcaac cattctgatt gattctttgc
    16501 ttatgcctga tgatttctcc atcttagcgg atgatgcaat ttggagtaat cgtttggatt
    16561 cgataatcct atactccggt gacgaagaac aatatttcgt caggtttatg cccgaatacc
    16621 cgggtaggta catggattct gccagcatct ttattaatct tcatcccgat ccacacatcc
    16681 tgaaagtgat gggtgaaggg atccaaccct tagaaattga tatttcttcc aactgttcag
    16741 attccgtttt tgcaggtcaa ttatattctt gcagaattga gattgagtca cataatggat
    16801 tccctgtcga cgttcaaata tttgaaaaac ctaattggtg caatcttaac aatcaatccg
    16861 atacattgct ggaaatttca ggcgctcctg tatattccga catcgggcaa catgaattac
    16921 atattcgggt tggtgatggc ctaatattta atgagttcta ccaggaaatc gatgtaattg
    16981 aacccattat tccttacgtt gagtacaact gtcctctttc aataaatgtc gaacaaatct
    17041 ttaattgctt gattagcgct ttaggaaaca accatcttcc tgtaaatatc gaaatcataa
    17101 attatccgaa ttggatagac tggaatcaaa aaagcgatga tacgattgag gtatatggca
    17161 ctccggggat aaatgatatt ggatctgcac tatttgagtt cagtgctttc gacagcctgg
    17221 cagagcatac aatatccttt actgtggtca ttgggccaac cggaacactt tctccggatg
    17281 atataatata tttccaaaga tttatcattt ctccaaatcc atccaaagat ttattttatt
    17341 tggcctttca tgatgataat cttccaaatg aattagaaat aggaatttat agctgcactg
    17401 gaaccaggat tgatcatctc tttatcaatg aaacttgtaa tactgttcca attaatctcc
    17461 aaaacctgcc cgccggaatc tactttgtcc gcctgaccat cgatgggaaa gaaacggtaa
    17521 c
//
LOCUS       DAOPNH010000031        17457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3168_length_17457_cov_6.546431, whole genome shotgun sequence.
ACCESSION   DAOPNH010000031 DAOPNH010000000
VERSION     DAOPNH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_17457_cov_6.546431"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKZ74_02945"
     CDS             <1..437
                     /locus_tag="PKZ74_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91740.1"
                     /translation="DNDPASFTLISDPPYMEAPTDWTEMYYDLSAYDGQTVYVAIQGV
                     TADAFIFMVDDISIDYYTGTPEIGNVNFSVYPNPVVNDMTITSDSEMNRIEIFNQLGQ
                     KVFARDIKEFKYRVNASEFENGVYYIRITAETGVTTQKVTIK"
     gene            601..3420
                     /locus_tag="PKZ74_02950"
     CDS             601..3420
                     /locus_tag="PKZ74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPM91741.1"
                     /translation="MMKKLLLIFVGLAAMSYLQAQEGWIEKNSNLTVGNGIGQISVGM
                     LDQTALWALPVDATGAIVDGFTRSLDGGQTWYPGTFNAGDGLSQLFAINSTTCWAVFN
                     TGATQGLYKTENAGATWTKKGNAYGSSSFANILHFFNEDDGVAQGDPVGGYYEIYTTS
                     NGGETWTRVPSINIPAPTSGEYGITGNYDAVGDHIWFGTNKGRIFHSADKGLHWTASL
                     TTFGATTVVQPEFSDELNGMAFRSYLDLGLEPTLSITHDGGATWESVNVAGPMYARYI
                     TYVPGTTATFVGSASAEGENGISYTTDGGYTWAAITEGYDFMASVWLDNETGWAGSFA
                     RSSRSVGGMYIYDGPPLEAFEVPGIAFNPSEIVEQAEAGGAESQILTVSNTGGVDLVY
                     SAHVVYVQPAKSAPQTILAAQPSPVRENGGSNVISTDPSYRPAAYNPPTDDFLLHYDG
                     NNADAFSWGPNLPYTIVNAAMFPPELTLPHAGMMLTSVIMYFNMTGDDHRVRVYGPGN
                     GSGPGEMLVDQPFTPTAESWTEVELDNPVYITGGELWIGYQYTQPVADMFIPGVDEGP
                     NNINGDWISFDGVVWNHLTDYESNVNWNIRGMLTGEPVAQWLTISSDGGTIEAGGSDQ
                     ITVTCDAADLAPGTYHAAIRFLSNDPDFPSVDVPVTFEVTESGSFQSVILDFEAQEDW
                     SLTFDPWITVDVDGSATYGIDGVEFPHAGEPMSFIAFNPATTEPSLADDPELQPHGGV
                     RFGACMASASPTYENDDWLISPAVTLGMNSSLTFWVKTYVDDWGLERYNVLISTTDNN
                     PASFTLISDPPYMEAPTDWTEMYYDLSAYDGQTVYVAIQGVTADAFIFMVDDISIDYW
                     VGTPETEEVSFSVYPNPVVNEMNITSNTEMTRIEIFNQLGQKVFARDMNDLRYKVNAA
                     EFNNGVYTIRITTESGVTTKKVTIN"
     gene            complement(3559..3717)
                     /locus_tag="PKZ74_02955"
     CDS             complement(3559..3717)
                     /locus_tag="PKZ74_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPM91742.1"
                     /translation="MAKKTFVLRLNPETYEAIEKWAADEFRSVNGQLEWIIDQALSSA
                     GRKKKAKS"
     gene            complement(3737..4591)
                     /locus_tag="PKZ74_02960"
     CDS             complement(3737..4591)
                     /locus_tag="PKZ74_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPM91743.1"
                     /translation="MEKEVNFKPKSGYIFLALVILVLGAIISMAFIADQGWVFAFLVI
                     PFFILPGFMVVNPNESMVLILFGDYIGTIKENGFFWANPFYVKKKISLRARNLDSEPI
                     KVNDKVGNPIMIGIVLVWKVEDTFKAAFEVDDYAHFVEIQSESAIRKLAGHYPYDNFD
                     DAQSEISLRSGGEEVNHVLENELRERLAIAGIHVIEARISYLAYAAEIAGAMLRRQQA
                     TAIVAARIKIVEGAVSMVEMALEQLSKKHIIDLDEDKKATMVSNLMVVLCSDKDAAPV
                     INAGTLHQ"
     assembly_gap    4726..4825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5081..5485
                     /locus_tag="PKZ74_02965"
     CDS             5081..5485
                     /locus_tag="PKZ74_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPM91744.1"
                     /translation="MLERDLSNQIIRSFYKVYNTLGYGFLEKVYENALYLELINNGIY
                     VKKQCPVQVYYDGTIVGNYYADIIVEDKIILELKSASTMAPEHEYQLINYLKATEIEV
                     GILLNFGKEPQFSRKIFTNDRKGVKRNTDDAD"
     gene            5573..6283
                     /locus_tag="PKZ74_02970"
     CDS             5573..6283
                     /locus_tag="PKZ74_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPM91745.1"
                     /translation="MITKKYNHLFIDLDKTIWDFESNTKVTFNEIFEKYSLNKLGIPD
                     VNTFYVRYEVINNLLWGLYRENRIKKEVLNIRRFELTLNEFGIDDLILATHLAEDYVT
                     FSPQKTLLFPGAHEALALLQTKHKLHIITNGFEELQQQKLDNCDLRKYFTTITTSEEA
                     GVKKPETRIFELALKKAGASVEESLMIGDDLEVDVAGARCIGMDTLFFNPEKVEHQDD
                     PDHEVHSWKEVVKLLVNS"
     gene            complement(6344..7048)
                     /gene="pyrH"
                     /locus_tag="PKZ74_02975"
     CDS             complement(6344..7048)
                     /gene="pyrH"
                     /locus_tag="PKZ74_02975"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPM91746.1"
                     /translation="MRYNRILLKLSGESLMGKASYGIDPERLNDYSEEIAAASRENVQ
                     IAIVIGGGNIFRGMQGSARGFDRQTGDYMGMLATVINSLVLRSSLEEKGVKVKLMSGI
                     YIEGLTEKANGMTAKQYLEDGYVVIFSGGTGNPFFTTDTASALRAVEISADAMFKGTR
                     VDGVYTDDPEKNPEAIKYDHLTFHEAFVKELKIMDLTAFTMCEENKLPVIVFNMNIKG
                     NLIKLLNGEKIGTRIE"
     gene            complement(7057..10392)
                     /gene="secA"
                     /locus_tag="PKZ74_02980"
     CDS             complement(7057..10392)
                     /gene="secA"
                     /locus_tag="PKZ74_02980"
                     /inference="COORDINATES: protein motif:HMM:NF009536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPM91747.1"
                     /translation="MNMNLLKALFGDKSTRDIKEINPILGKIKSAYEEISRLSNDGLR
                     EKTLDFRRKIAEAIREEEKKIEELKAAIELEEDIMEREKMWEQIDKHEKESYRITQEV
                     LNEILPEAFSVMKETAKRFKENKEVIVTATEYDRELAEKKDSVIISGDKAHYKNQWMA
                     GGNIITWDMVHYDVQLIGGMVLHQGKISEMATGEGKTLVATLPVYLNALPGKGVHIVT
                     VNDYLARRDSEWMGTLYEFHGLKVDCIDKHQPNSVARRNAYLADITFGTNNEFGFDYL
                     RDNMTGNPDELVQRGHNYAIVDEVDSVLIDDARTPLIISGPTPKGDNQEYENYKPQIE
                     RLYSMQRTLVTKILAEAKRLLTDNAPPEDEKKGGELIFRAYRGLPKNNALIKFLSEPG
                     MKALMLKTENFYLQEQAKNMHVIDDELYFVIDEKHNSIDITEKGIDLLTEYTGDPNFY
                     LLPDIGSELAEIEKLPLKDTEKMEKKETLMQDFSVKSERVHTVNQLLKAYSLFEKDVD
                     YVIMDNKIKIVDEQTGRIMEGRRWSDGLHQAVEAKENVRVEAATQTYATITLQNYFRM
                     YGKLAGMTGTAETEAGELWDIYKLDVVVIPTNRPIVRDDREDLVYKTKREKFNAVIKE
                     IEELVAKGRPVLVGTTSVETSELLSRMLTRVKIRHNVLNAKLHQKEADIVAEAGKAGT
                     VTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQF
                     FVALEDDLMRMFGSDRIAKIMDRLGIKEGEVIQHSMITKSIERAQKKVEENNFGIRKR
                     LLQYDDVMNSQREVIYKKRRHALFGDRLAVDISNMIYDVGEQMINQYLEEKDFEGFKL
                     ALLRTMSIESPFSADEFFSMRTETMADQLYDAAYKHYVEKSKRIAELAYPVIKDVYEN
                     QTQYENIAIPITDGIKTMNVVANLKRSYENGGKEIILSIEKGITLAMIDEAWKEHLRE
                     MDDLKQSVQNATYEQKDPLLIYKFEAFELFKKMVARINEEITSFLIRGKLPLQDASSV
                     KEARAPRGIDRSRMREERKDLLAQSHSDTQANRPNEPMKRDIPKIGRNDKVKVQYGDG
                     RIIVAKFKAIEHDLNRGNCQIIN"
     gene            complement(10525..11898)
                     /locus_tag="PKZ74_02985"
     CDS             complement(10525..11898)
                     /locus_tag="PKZ74_02985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPM91748.1"
                     /translation="MKKSILLCIFLLTFAINGFSDLTTISGTLKNGSGYTVRLMTYND
                     QLSYIRKTLASTVAGEDERFSLSVDISAVTYCWIDIEFQQAELFIQPGQSYEVEIDLA
                     GDPLSMSYYNRRTLDNRIIRDDSMQLNNSIQDFNEIYNDFLLNISDNIRQQNSRVAYE
                     TFMTAVELRFQGISSEYFDNYIRYKSASMQLFMRIKSREKIGTEFLADRPVLFNHLEY
                     MDFFHLYFDKYFVTNGKYFNYSKTYDMINGKAPLAEILDSLAVDPALKQRPAMELLLL
                     CGLKGLYSMPEFSKDRITALVKELTIAGSQPGIRELAANLLVRFKRLKPGSPAPGFAL
                     ETIAGGQKYSLPEFTGKKLYLAFFESDNPASQSELGQISEIYDEFKNDVSFVAVSVDK
                     NRDRLMEYIDKADAPWLFLHYGGDLDLLENYDAVAFPCFILIGADGKIIRCPAPSPSE
                     NIRKLFD"
     gene            complement(11933..12394)
                     /gene="coaD"
                     /locus_tag="PKZ74_02990"
     CDS             complement(11933..12394)
                     /gene="coaD"
                     /locus_tag="PKZ74_02990"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPM91749.1"
                     /translation="MERIAVFPGSFDPITKGHESIIRRAMPLFDKIIVAIGENAEKKS
                     FFSLEQRMDWIKQVFVNEPAIEVKSYKGLTVDFCIASKAKFLLRGLRTSADFEFERSI
                     GQINKNLNADVETVFLLTMPEHTALNSSIVRDILRNGGDPSQFVPEAVKFK"
     gene            12508..13149
                     /gene="pyrE"
                     /locus_tag="PKZ74_02995"
     CDS             12508..13149
                     /gene="pyrE"
                     /locus_tag="PKZ74_02995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPM91750.1"
                     /translation="MIYNEEIARTTAKSLLQIKAVKLNPTEPFTWASGIKSPIYCDNR
                     ITLSFHAIRTYIRQQFVHIIREEFGVVEYIGAVATGGIAQGALVAQEFGIPLVYVRSE
                     KKSHGMENKIEGKIEPGKSIVVIEDLVSTGSSSLTAVQALTEAGFIVKGMVAIFSYNL
                     EPARKNFADAKCRLVTLSDYNTLINEALDSNYITDDDLQSLVKWRENPEKWGK"
     gene            13137..13550
                     /locus_tag="PKZ74_03000"
     CDS             13137..13550
                     /locus_tag="PKZ74_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91751.1"
                     /translation="MGKVTFQSQQATLPVTAGEVYLFFYDLNNLRKLMPEQIINWQSD
                     TDKCSFDIKGMAHLDLQRGETEPDRLVKIISAGDSPIGLEIRGISEDAGENRAVARIE
                     IAAELSPMLQMMVSSPLQNLVKMMAERLEAEMRGS"
     gene            complement(13605..14360)
                     /locus_tag="PKZ74_03005"
     CDS             complement(13605..14360)
                     /locus_tag="PKZ74_03005"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPM91752.1"
                     /translation="MMGTIGNEIIRLHEVDSTNRYLMDWINRENVSEGTVVITDFQTA
                     GRGTDGARWESEQSKNLTFSLLLKPVFLAPEAQFYLNKVISLGLADAVNELLPGRTDI
                     RVKWPNDLYIGDRKVAGTLIQNGVKGSRFEFSILGIGLNVNQSRFTDDAANPVSLNQV
                     SGKIFDLAEVLELVLKKIEGRYECLKKGDKTCVDADYLKVLYRFNEMARFIVKEKPVE
                     ARITGVNRYGQLILEIPGSKILECDLKEVKFEI"
     gene            14489..14860
                     /gene="rsfS"
                     /locus_tag="PKZ74_03010"
     CDS             14489..14860
                     /gene="rsfS"
                     /locus_tag="PKZ74_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPM91753.1"
                     /translation="MTPELYESEQLTELIIQGMEDTKAKKIVILDLQDIDSAVTNYFV
                     ICHGNSKPQLEAIADSVIAKTVKGLKAKPWHKEGFENAEWILLDYVDVVVHIFREDRR
                     EFYQLEKLWADAPITRHDYKE"
     gene            14879..16930
                     /gene="ftsH"
                     /locus_tag="PKZ74_03015"
     CDS             14879..16930
                     /gene="ftsH"
                     /locus_tag="PKZ74_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPM91754.1"
                     /translation="MAEENLNKQDKGGAQQPKGNNKSKYFGYWWIGLVFILLLTYLVN
                     WDTGTAKKATWGEVKEMLATGDIERIVLVNKEAAEFYLKNEKLDDPKYKEIKETVAAK
                     GPHYTYNITSPESFERMVAEAQEGIADPIYIENDVRRNWTGEVLSWIVPIAILIIIWL
                     VIMRMMSRNAGGPGGQVFNIGKSKAQLFDKTTSVSINFNDVAGLEEAKLEVKEVVDFL
                     KNPSKYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVG
                     ASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGENQITGANDERESTLNQLLTEMDGFS
                     TNSGVIIMAATNRADILDRALLRAGRFDRQINVELPDLDERRAIFAVHTKNLKFDKTS
                     VDLEWLARQTPGFSGADIANVANEAALIAARNNKKIIEKQDFIDAVDRIIGGLERRSK
                     IISQEEKRVIAFHESGHAAVSWLLPHAHPLVKVTIVPRGKALGAAWYLPEERQITTFD
                     QMFDEMTATLGGRAAEEIIFGKVSTGALNDLERVTKQAYNMVVFFGLNEKLGNISYYD
                     STGQQEYMFNKPYSEKTAEIIDQEIGKLIDQAYKRAVEILRTHKDKLEALASILLEKE
                     VIFTEDLETIFGKKIKHVGHEVVLADPNGNGHVEAPAVVEEAKPKKRGRKPGSVGQLD
                     S"
BASE COUNT         4439 a         4245 c         4148 g         4525 t
ORIGIN      
        1 cagataatga tccggccagc tttaccctga tttccgatcc accctatatg gaagctccga
       61 cagactggac tgaaatgtac tacgacctgt ctgcttatga cggacagact gtttacgtcg
      121 ctatccaggg tgttacagcc gatgcgttca tctttatggt ggatgacatt tccatcgact
      181 actatacggg aactcctgaa atcggtaatg ttaacttctc ggtttatcct aatccggttg
      241 ttaacgatat gacgattacc agcgattcgg aaatgaacag gatcgaaatt ttcaaccagc
      301 tcggacagaa agtgttcgcc agagacatca aagaattcaa atacagagtg aatgccagcg
      361 aatttgagaa tggagtgtat tatatccgga tcaccgctga aaccggagtt actactcaaa
      421 aagtaactat taaataagca tttctttcat tattaatgcc ataagacaag aggctgcccc
      481 tgattcagag gcagcctctt ttttttgttt taccattaac attaatgact ttatattttg
      541 ttggtttgta ttgaaagatt tatccataaa tttgaatcat taaattctaa ttcaaacaat
      601 atgatgaaaa aattactatt gatttttgtc gggcttgcag ccatgtctta tttgcaggcc
      661 caggaaggat ggattgagaa aaattccaac ctgacagtcg gtaacggtat cggacagatc
      721 tccgtcggaa tgctggatca aacagccttg tgggctctgc ctgtcgacgc taccggcgct
      781 atcgtcgatg gctttacgcg ttcactcgac gggggccaga cctggtatcc cggtacattc
      841 aatgccggcg acgggctgtc gcaactcttt gccatcaatt ccacaacctg ctgggccgtg
      901 ttcaacaccg gagccaccca gggactttac aaaaccgaaa acgccggggc cacctggacc
      961 aaaaaaggta atgcatatgg cagctcatcc tttgccaata ttcttcactt ctttaacgaa
     1021 gacgacggag ttgcacaggg tgatccggta ggaggatatt atgaaatcta caccaccagc
     1081 aatggcggtg agacctggac acgcgttcct tcaatcaata tccccgcacc taccagcggt
     1141 gaatacggta ttaccggaaa ctatgacgcc gtgggcgatc atatctggtt cggtaccaat
     1201 aaagggagga tcttccattc tgctgacaaa gggctccact ggacagcttc gctgacgacc
     1261 ttcggcgcaa cgaccgtcgt acagcccgag ttctcggatg aactgaacgg catggcattc
     1321 cgttcctatc tggatctcgg tcttgagcct acattaagca ttactcatga cggtggcgct
     1381 acctgggaat cggtcaatgt agccggcccg atgtatgccc gctatatcac ctatgttccg
     1441 ggaacgacgg ccactttcgt cggatcagcc agtgcagaag gcgaaaacgg catatcttac
     1501 accacggatg gtggttatac ctgggcggct attacagaag gctatgattt catggcatcg
     1561 gtatggcttg ataacgaaac aggttgggca ggttcatttg cacggtcaag ccgcagcgtt
     1621 ggaggtatgt acatttacga cggacctccg ctggaagctt ttgaagtacc cggaatcgct
     1681 ttcaatccct ctgaaatcgt cgaacaagcc gaagcaggcg gcgccgaatc acaaatcctg
     1741 accgtatcaa ataccggcgg agttgacctg gtctacagcg ctcatgtagt ttatgtccag
     1801 cctgctaaaa gcgctccgca gactattctt gctgcccagc cgtctcctgt cagggaaaat
     1861 ggaggcagca acgtgatctc caccgatcct tcctaccgcc cggccgctta taatccgccg
     1921 acggatgatt tcctcctcca ttacgacggt aacaatgccg acgctttctc ctgggggccg
     1981 aacctgccct ataccattgt taatgcggct atgttcccgc cggaacttac ccttccgcac
     2041 gccggtatga tgctgacctc ggtgattatg tatttcaaca tgaccggcga tgatcacagg
     2101 gtacgtgtct acggtcccgg taatggcagc ggtcctggcg agatgcttgt tgatcagcca
     2161 tttacaccta cagctgaatc ctggaccgag gtagaactcg ataatccggt ctatattaca
     2221 ggtggcgagc tctggatcgg ttaccaatat acccagcctg tagcggatat gttcattccc
     2281 ggtgtcgatg aaggtccgaa taatatcaac ggcgactgga tctctttcga cggcgtggtg
     2341 tggaaccacc tgaccgacta cgaatccaac gtcaattgga atattcgcgg gatgcttacc
     2401 ggcgagccgg tagctcaatg gctgactatt tcatccgatg gcggtacaat tgaagcgggt
     2461 ggcagcgatc agatcaccgt tacctgcgat gcggccgacc tggcacccgg aacctatcat
     2521 gcagctatcc gcttcctgag caacgacccg gatttcccat ccgtagacgt tccggtcaca
     2581 ttcgaagtta ctgaatcagg atcattccag tctgttatcc tcgacttcga agcccaggaa
     2641 gactggtcgc tgacttttga tccatggatc actgttgatg tcgatggatc cgctacttac
     2701 ggtatcgacg gtgttgaatt ccctcatgcc ggcgaaccca tgtcgttcat cgcttttaac
     2761 ccggcgacga ctgagccgtc tttagccgat gatccggaac tccagccaca tggcggcgtc
     2821 cgcttcggcg cttgcatggc atcggcgtca ccgacttacg aaaacgatga ctggctgatc
     2881 tcaccggcgg tgactctcgg aatgaattcc tctcttacct tctgggttaa gacctatgtc
     2941 gatgactggg gcctggaaag atacaatgta ctgatctcca ctaccgataa caatccggcc
     3001 agctttaccc tgatttccga tccaccatat atggaagctc caaccgactg gacagaaatg
     3061 tactacgacc tctccgccta tgacggacag accgtgtatg tcgctatcca gggcgttacg
     3121 gccgatgcat tcatctttat ggtggatgat atttcgatcg attactgggt cgggacgcct
     3181 gaaacggagg aagtatcctt ctcggtctat cccaatcctg ttgtcaatga aatgaatatc
     3241 accagcaata ctgaaatgac aaggattgaa attttcaacc agcttggcca gaaggtgttt
     3301 gcaagagata tgaatgatct caggtacaag gtgaatgccg ccgaatttaa caacggcgtt
     3361 tacaccattc ggatcacaac cgaatcagga gttactacta agaaagtgac gatcaactaa
     3421 gctgattcga tcataacttt atgcaaaagg ctgccttttt tagagggcag ccttttttaa
     3481 ttctgcgact aaagccgtcg gttcacgaaa ggtcttcttc cgacgactaa agccgtcggt
     3541 tcaaagacct ttttattgtc aggatttagc tttctttttt cttcctgcag acgataatgc
     3601 ctggtcgatg atccattcga gctggccgtt tacgctccgg aattcgtcgg cagcccattt
     3661 ttcgatggct tcataggttt ccggattcaa tcgcaataca aaagtttttt ttgccatccc
     3721 cggtcagatt tagtttttat tgatgcaatg taccggcatt aattaccgga gcggcatctt
     3781 tatcagagca taatacgacc atcaggttgc tgaccatcgt cgctttttta tcttcgtcga
     3841 gatcgatgat atgctttttg gagagttgct ccagggccat ctcgaccatg ctgacggctc
     3901 cttcgacgat tttaatacgg gcagccacaa tagcggtggc ttgctgcctg cggagcatgg
     3961 cgccggcgat ttctgcagca taagccaggt agctgatacg ggcctcaatc acgtggatac
     4021 cggcaatggc aagccgctcc cgcagctcat tctccagtac atgattcacc tcttccccac
     4081 ccgaacgcag actgatctcc gactgggcat cgtcgaaatt gtcgtaggga taatgacccg
     4141 ccagtttccg gatagccgat tcgctctgaa tttccacaaa atgtgcgtaa tcatccacct
     4201 caaacgcggc tttgaaggtg tcttccactt tccagaccag gacaataccg atcataattg
     4261 ggtttccaac cttatcgttc actttgatcg gttcgctgtc gaggttgcgg gccctgagcg
     4321 aaatcttttt ctttacataa aacggattgg cccaaaaaaa tccgttctct ttaattgttc
     4381 cgatgtaatc gccgaatagg atcagcacca tcgattcatt gggattgacc accataaatc
     4441 ccggcaggat aaaaaacggg atcaccagga atgcaaatac ccagccctga tcggcaataa
     4501 aagccattga aataattgct cctaaaacaa ggatcaccag ggccaggaag atgtaaccgc
     4561 tcttgggttt gaaattgact tctttttcca tttttaatct attttaatat tattttgata
     4621 ttgcaataat actatttttt tcattacaaa ttattttttc ttttttttaa tgaacgcgga
     4681 tgacacggat agtacggatg ggcacgaata aatacccgtt ttaatnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnttcta acttttcggt tctgaacgcg gatgacacgg
     4861 atagtacgga tgggcacgaa taaatacccg ttttaatccg tccaatccgt tttattcgtg
     4921 ttctaacttt tcggttctga acgcggatga cacggatagt acggatgggc acgaaaaatt
     4981 ggtgattatg gattcttata atccgcatca atcaaaggtt ttaaaaaata ttttttattg
     5041 accgggtatt tttattcaat ttttcggatt aacaaataaa atgttagaaa gagatctatc
     5101 aaatcagatt attcgaagct tctacaaagt atataatact ttaggctatg gattccttga
     5161 aaaagtctat gagaatgcac tttatctgga attaatcaac aatggtattt acgttaaaaa
     5221 acaatgcccg gttcaggttt attatgacgg aactattgta ggcaattact atgctgatat
     5281 cattgttgag gacaaaataa ttcttgaatt aaaatcagca tcgacgatgg ctccagaaca
     5341 cgaataccag ttgatcaact atttaaaagc aactgaaatt gaagttggga tacttttaaa
     5401 tttcggcaag gagccccaat tcagcaggaa aatattcaca aacgatagga agggagtaaa
     5461 aaggaacacg gatgacgcgg attaaacgta tgaacacgaa taagatcggt gaaaatccgt
     5521 cccatttgtc tcattcgtgt tctatttatt ttgattaagg tatttttgcc ggatgatcac
     5581 aaaaaaatac aatcaccttt ttatcgacct ggataaaact atctgggact tcgaaagcaa
     5641 caccaaagtc accttcaacg aaatttttga aaaatattca ctgaacaaac tgggtattcc
     5701 cgatgtaaat accttttatg tcaggtatga agtgatcaac aacctgttat gggggcttta
     5761 ccgggaaaac cggataaaga aggaagtgct gaatatcagg cgttttgagt tgacgctgaa
     5821 tgaattcggt attgatgacc tgatactcgc aacccatctt gccgaagatt acgttacttt
     5881 cagcccgcag aaaacactgt tgttccctgg tgctcacgaa gcgctggcac tcctgcagac
     5941 aaaacacaaa ttgcatatca ttaccaacgg gtttgaagag ttgcagcagc agaagctcga
     6001 caactgcgac ctacggaagt attttacaac tataaccaca tcggaggagg ccggcgttaa
     6061 aaagcctgaa acccggatct tcgaactggc tttaaagaag gccggtgcat cggtcgaaga
     6121 gagtttaatg atcggtgatg acctggaggt ggatgttgcc ggcgccagat gcatcggaat
     6181 ggatacttta ttttttaatc cggaaaaagt tgaacaccag gatgatcctg atcatgaggt
     6241 ccattcatgg aaggaagtgg tgaaactatt ggttaattct taatttttac tgctaatttt
     6301 tttttataaa atagctcaaa atatgactaa accgggttta tagctattcg atccgtgttc
     6361 cgattttttc tccgttgagg agtttgatca ggttaccctt aatattcata ttaaaaacaa
     6421 tgacgggaag cttattttcc tcacacattg taaatgcagt aaggtccatg atcttcaatt
     6481 ctttaacgaa cgcttcatga aaagtcaggt ggtcatattt tatcgcttcc gggtttttct
     6541 cgggatcgtc ggtataaacc ccatcaacgc gtgtaccctt gaacatggca tcggctgaaa
     6601 tctccactgc tctaagcgcc gaggcggtat cggtggtaaa aaagggattc ccggtacctc
     6661 cgctgaaaat aaccacatag ccatcctcca ggtattgctt tgcggtcatc ccattggcct
     6721 tttccgtcaa tccttcgata taaatacccg acatcagttt gaccttaacg cctttttctt
     6781 ccagcgaaga tcgcaggacc aggctattga taacagttgc cagcatgccc atataatcgc
     6841 cggtctggcg gtcgaatccc cgggcgctgc cctgcattcc gcggaaaata tttcctccgc
     6901 caattacaat ggctatctga acgttttccc gtgatgcagc agcaatttct tccgaataat
     6961 cgtttaaccg ctcgggatca ataccatagg atgcttttcc catgagggat tcaccactta
     7021 gctttaaaag aattcgattg tatctcatct ttttatttag ttgataatct gacaattgcc
     7081 ccggttcagg tcgtgttcga tggccttgaa cttagctaca atgatccgac cgtcgccgta
     7141 ctggactttc actttatcat tcctgccgat tttggggata tcgcgtttca tgggctcatt
     7201 cggccggttt gcctgggtat cgctatgcga ctgggccagc aggtcttttc tctcctcccg
     7261 catccttgaa cggtcgatgc ctcttggcgc ccgggcttcc ttaaccgaag aggcatcctg
     7321 cagcggaagt ttaccacgga tcaggaagga ggtgatctct tcgttgatgc gggcgaccat
     7381 ctttttaaac aattcgaaag cttcaaactt gtatatcagc aatggatctt tctgctcata
     7441 ggtagcattc tggaccgact gcttcaggtc gtccatttcg cgaaggtgct ctttccaggc
     7501 ttcatcgatc atggccaggg taattccttt ctcgatggag aggataatct ctttgccccc
     7561 gttttcgtag gatcgcttca gattggccac aacattcatc gtcttgatcc cgtcggtgat
     7621 cggaatggcg atattttcgt actgggtttg attttcgtac acatccttga tgaccgggta
     7681 ggccagttcg gcgatccgtt tcgatttctc tacgtaatgc ttgtatgcgg catcataaag
     7741 ctggtcggcc attgtttcgg tccgcatgga aaagaattca tccgcactga acggcgattc
     7801 gatcgacatg gtccggagca gcgccagttt aaagccttcg aaatcttttt cttccaggta
     7861 ctggttgatc atttgttcgc cgacatcgta gatcatattg gatatatcga cggcgagcct
     7921 gtcgccaaaa agcgcatggc ggcgtttctt gtagatgact tcccgctggg agttcatcac
     7981 gtcgtcgtat tgcagcagcc ttttcctgat gccgaagttg ttctcctcta ctttcttctg
     8041 cgcccgctcg atcgatttgg tgatcatcga gtgctgaatc acttcacctt ctttgatccc
     8101 cagccggtcc atgatcttgg cgatacggtc cgatccgaac atacgcatca ggtcgtcttc
     8161 aagggcaaca aagaactgcg aagaaccggg atctccctgg cggccggcgc ggccccgcag
     8221 ctgccggtcg acgcggcgcg actcatggcg ctccgttccg atgatggcaa gacctcctgc
     8281 ttctttcact cccggtccga gcttgatatc ggtaccacga cctgccatgt tggtggcaat
     8341 ggtcaccgtt ccggccttac cggcctcggc tacaatgtct gcctctttct gatgaagctt
     8401 ggcattcagc acgttatggc ggatcttgac acgggtgagc atccggctca acaattccga
     8461 agtttcaaca gaggtggttc caaccagtac cggacggcct ttggccacca attcctcaat
     8521 ttctttaatt actgcgttga acttttcgcg ctttgtctta tataccaggt cttcgcgatc
     8581 gtcacggact atcggccggt ttgtcggtat gaccacgaca tccaatttgt aaatatccca
     8641 gagctcgccg gcttccgttt cagccgtacc ggtcataccg gccagcttgc catacatcct
     8701 gaaatagttc tggagggtaa ttgtggcata tgtttgcgtc gccgcctcca ctctcacatt
     8761 ctctttcgcc tcgacagcct ggtgcaaacc gtcggaccag cggcggcctt ccataatacg
     8821 cccggtctgc tcgtcgacaa tcttgatctt gttgtccatg atcacataat cgacatcttt
     8881 ctcaaacagc gagtaggctt tcagcagttg gttcacagtg tggacacgtt ccgatttcac
     8941 ggagaaatcc tgcatcaggg tctctttttt ctccattttc tcggtgtctt ttaaaggcag
     9001 cttttcgatc tcggccagtt ccgatccgat atcgggcagc aggtagaaat taggatcacc
     9061 cgtgtactcc gtcagcaggt cgatcccttt ttcggtgata tcgattgaat tgtgtttttc
     9121 atcgatgacg aaatagagtt catcatcgat aacatgcata tttttggctt gttcctgcag
     9181 ataaaagttc tcggtcttga gcattagggc tttcatcccg ggctcgctga ggaatttgat
     9241 cagggcattg tttttcggaa gcccccggta agccctgaag atcagctcac cacccttttt
     9301 ctcatcctcg ggcggggcat tatccgtcaa taaacgtttg gcttcagcaa ggattttcgt
     9361 caccaaagtg cgctgcatgg aataaagccg ctcaatctgc ggtttataat tctcatattc
     9421 ctggttatcg cctttgggtg taggccctga aataataagc ggtgtacggg cgtcgtcgat
     9481 caaaacggag tcaacctcgt cgacgatggc gtaattatgc ccgcgctgta ccagttcgtc
     9541 gggatttccg gtcatgttgt cgcggaggta atcgaagccg aattcgttgt tggttccgaa
     9601 agtgatatca gccaggtaag cattccggcg ggcgacgctg ttcggctggt gtttatcgat
     9661 gcaatcgact ttgaggccgt ggaattcata gagtgttccc atccattccg agtcacgcct
     9721 tgccaggtaa tcgttaaccg ttacgatgtg cacccctttg cccggtaaag cattgaggta
     9781 aacgggaagg gttgcaacca gcgttttccc ttcaccggta gccatttccg aaattttccc
     9841 ctggtgcagg accattccgc cgataagctg tacgtcgtag tggaccatgt cccaggtgat
     9901 aatattcccc ccggccatcc actggttttt gtaatgagct ttgtcgccgg agatgatgac
     9961 actgtccttt ttttccgcca gttccctgtc atattccgtt gctgtaacga tcacttcctt
    10021 gttttccttg aatcgctttg cagtttcctt catcacgctg aaggcttcag gcaggatttc
    10081 attcagaacc tcctgcgtga tccggtaact ctccttttca tgcttatcga tctgctccca
    10141 catcttttca cgctccatga tatcctcttc caattcgatg gcagccttca actcttcgat
    10201 cttcttttcc tcttcacgga tggcttctgc aatcttcctc cggaaatcga gcgttttctc
    10261 cctgagcccg tcgttgctta accgggaaat ctcttcataa gctgatttta ttttaccaag
    10321 aatcggattg atctctttaa tatcgcgggt cgacttatcg ccgaataatg cctttaataa
    10381 attcatattc aataaaattt ggcctgaagg ctcaataatt gttccttact ataggataac
    10441 atgttgatga cataatggca ggaagaacct gtattacagt acaaagttaa ggcaatttac
    10501 gaatcaaact ccaatcggac cgaatcagtc gaacaacttt cggatgtttt cgctgggact
    10561 gggcgccggg caacggatga tcttaccgtc ggccccgatc aggataaagc aggggaatgc
    10621 caccgcatca taattttcca gcaggtcaag gtcgccgccg taatgcagga aaagccatgg
    10681 agcatctgct ttgtcaatat attccatcaa tcggtcccga ttcttatcca cagaaacagc
    10741 cacaaatgac acatcattct taaattcatc atatatctct gaaatctggc ccagttccga
    10801 ctggctggcc gggttatccg actcgaagaa tgccaggtaa agttttttcc cggtaaattc
    10861 gggcaaggaa tatttctgac cgccggcgat ggtttccagg gcaaaaccgg gcgcaggtga
    10921 accgggcttg agtcttttaa aacggacgag caaattagcc gcaagttccc tgatcccggg
    10981 ctgggagccg gcaattgtca gctcttttac cagtgcggtt atccggtctt tactgaattc
    11041 gggcatcgaa tagagcccct tcaatccgca caacagcaac aactccatgg caggccgctg
    11101 ttttaaagcc ggatcaaccg caagcgaatc cagtatctcc gccagcggcg ctttcccgtt
    11161 gatcatatcg taggtcttgc tataattgaa atatttgcca ttggtcacaa aatatttatc
    11221 aaaatagaga tgaaagaaat ccatatattc gagatggtta aaaagaaccg gacgatcggc
    11281 caggaattcg gtcccgatct tttccctgct ttttatccgc ataaataatt gcatggaggc
    11341 cgatttgtag cggatataat tatcgaaata ctcgctgctt ataccctgga atctcagttc
    11401 caccgcagtc atgaaggttt cataagccac cctggaattc tgctgtcgaa tgttatccga
    11461 tatattcagt aggaaatcgt tgtatatctc gttgaaatcc tgaatggaat tatttaactg
    11521 catcgaatcg tcgcggatga tacgattatc gagggtacga cgattgtaat aagacatcga
    11581 cagcggatca ccggccagat cgatctccac ttcgtacgat tgaccgggct gaataaacaa
    11641 ttcggcttgt tgaaattcga tatcgatcca gcaataggtc acagcagata tatcgacact
    11701 taaggaaaac ctctcatcct caccggccac agtgcttgca agcgttttcc tgatatacga
    11761 caactggtcg ttatatgtca tcaaccttac ggtataacca ctcccgtttt taagggtccc
    11821 ggaaatggtg gtcaagtcac taaagccatt aatggcaaag gttaatagaa atatacagag
    11881 caagattgat tttttcatac ccaatttcta tactaacgaa agacaaggcc tgttatttga
    11941 attttaccgc ctccggaacg aactgtgaag gatcgccgcc gttcctgaga atgtctctga
    12001 ctatggaaga gttcagggcc gtatgttcgg gcatggtcag caggaaaaca gtttcaacgt
    12061 cggcattcag gtttttattg atctggccga tgctgcgttc gaattcaaaa tcggcggagg
    12121 tacgcaatcc gcgcaggaga aactttgctt tactggcgat gcaaaaatcg acagtaaggc
    12181 ctttataaga cttaacctcg atggccggtt cattcacaaa gacctgtttt atccaatcca
    12241 ttcgttgttc aagggagaag aagcttttct tctccgcatt ctctcctatg gccacgatga
    12301 ttttatcgaa cagtggcata gcccgcctga taatggattc atgacctttg gtgatcgggt
    12361 cgaaagaacc ggggaatacg gcaattcttt ccataccttg atttttaaac aaagatatat
    12421 taatttaggg tgatgaatac taaaccgcaa gccggtgaag ttatgaacaa attaacccgt
    12481 taatgattta ctttttactt ttgccttatg atttataacg aagaaattgc cagaacgacc
    12541 gccaagtcac ttctgcagat caaggctgtc aaacttaacc ctacagagcc ctttacatgg
    12601 gcttccggga tcaaatcacc gatttactgc gataaccgta taaccctgtc gtttcatgct
    12661 atccgtactt acatccgcca gcagttcgtt catataatcc gtgaagaatt cggcgtggtg
    12721 gagtatatcg gtgcagtggc tacaggcggg atagcccagg gcgccctggt ggcccaggaa
    12781 ttcggcattc cgctggtgta tgtcaggtcg gagaagaaat cgcacggaat ggagaacaag
    12841 atcgagggga agatcgagcc ggggaaatcg atcgtggtca tcgaggacct tgtatcgacc
    12901 ggcagcagca gcctgactgc cgtgcaggcg ctcaccgagg ccggattcat cgtcaaaggg
    12961 atggtcgcca ttttcagcta taacctcgaa ccggccagga agaactttgc cgacgccaaa
    13021 tgcaggcttg tgaccctgtc ggattataac acccttatca acgaagccct cgacagcaat
    13081 tacatcaccg acgacgacct gcagtcgctg gtgaaatggc gggaaaatcc tgaaaaatgg
    13141 ggaaagtaac ctttcaaagc cagcaggcca ccctcccggt aacggccggc gaggtttacc
    13201 tcttttttta tgacctcaac aacctgcgca agcttatgcc cgaacagatc atcaactggc
    13261 agtcggatac ggataaatgt tcgttcgata tcaaagggat ggcgcatctt gacttgcaac
    13321 gcggggagac agagcctgac cggctggtga agatcatttc cgccggcgac agtcctatcg
    13381 gcctggagat caggggcatc tccgaagatg ccggcgaaaa cagggctgta gcccggatag
    13441 agatcgcggc cgaactcagc ccgatgctgc aaatgatggt cagttcaccc cttcagaacc
    13501 tggtgaagat gatggcggaa aggctggagg cggagatgcg gggttcctag ttccaggttc
    13561 caggttccag gttccaggtt ccaaattttc aaattttcaa attttcaaat ttcgaatttc
    13621 acctctttca ggtcgcattc gaggatttta ctgcctggaa tttccaggat gagctgcccg
    13681 taacgattta cgcctgttat ccgggcttcg acgggctttt ctttaacaat gaaccttgcc
    13741 atctcgttga atctgtacaa aaccttcagg taatcggcat ccacacatgt tttatcgccc
    13801 tttttaaggc attcgtaccg tccttcgatc ttcttcagga ccaattccag gacttcggcc
    13861 aggtcgaaga ttttacccga cacctggttg agcgagacag gattggctgc atcgtcggta
    13921 aaccgggact ggttgacatt caacccgatc ccaaggatgg aaaactcaaa ccgagagccc
    13981 tttaccccgt tctggatcag cgttccggcc actttccggt cgccgatata gagatcgttc
    14041 ggccatttga cccggatatc ggtgcggccc ggcaaaagtt cattcacggc atctgccagt
    14101 ccaagtgaaa tgaccttatt caggtagaat tgtgcttccg gggccaggaa aaccggcttc
    14161 aggagcaggc tgaaggtgag attctttgat tgttcgcttt cccagcgggc gccatcggta
    14221 cccctgccgg ctgtctggaa atcggtgatc accaccgtgc cttccgatac attctccctg
    14281 ttgatccaat ccatcaggta gcgattcgtc gaatcgacct cgtgaagcct gatgatctca
    14341 tttccgattg tacccatcat taaacaattt tcacaaaatt acgttaataa gccggaagat
    14401 atgattcatt tttcaaataa cctaaacgat aagtggatca aaccgataga atgcaaaaaa
    14461 gcttaatttt gccgacaaat accagaccat gactccggaa ctatacgaat cagaacagtt
    14521 gacagaactc attatacagg ggatggagga cacgaaagcg aaaaagatcg tgatcctcga
    14581 tttacaggac atcgacagtg ccgtgaccaa ttattttgtg atctgtcacg gcaattccaa
    14641 accgcaactg gaagcgattg ccgattcggt tatcgccaaa accgtcaaag ggctgaaggc
    14701 gaaaccgtgg cacaaggaag gttttgaaaa tgcggaatgg attttgctgg attacgtcga
    14761 cgttgtcgta catatcttcc gggaagaccg gcgtgagttc tatcaactgg agaaactgtg
    14821 ggcggacgca ccgattacaa ggcacgatta taaagaataa ttcaaatatt aagacagaat
    14881 ggcagaggag aatttaaaca agcaggataa agggggagca caacaaccta aaggaaacaa
    14941 caaatcgaaa tatttcggtt actggtggat cgggctggta tttatactgt tgctgaccta
    15001 cctggtgaac tgggatacgg gcacagccaa aaaggctacc tggggtgaag tcaaggagat
    15061 gctggccacc ggcgacattg agcgtattgt gctggtcaac aaggaggcgg ccgaatttta
    15121 tctcaaaaac gaaaagctgg acgatccgaa atacaaggag atcaaagaga ccgttgccgc
    15181 caaagggccg cactatacct ataatatcac ctcccctgaa agttttgaaa gaatggttgc
    15241 cgaggcgcag gaaggcattg ccgatccgat ctacatcgag aacgacgtca ggcgcaactg
    15301 gacaggcgaa gtgctgagct ggatcgtgcc aattgctatt cttatcatca tctggctggt
    15361 gatcatgcgg atgatgagcc gcaatgccgg cgggccggga ggacaggtct tcaacatcgg
    15421 caaatcgaaa gcgcagttgt tcgacaagac caccagcgtc tcgatcaatt tcaacgatgt
    15481 ggcgggtctg gaggaggcca agctggaggt gaaagaggtg gtggacttcc tcaagaaccc
    15541 ctcaaaatac accgaactgg gcggcaaaat ccccaaaggc gtattgctgg tgggccctcc
    15601 ggggacgggc aagacattgc tggccaaagc cgtagccggc gaagcacagg tccccttctt
    15661 ctccctttcg ggttccgatt tcgtggagat gtttgtcggg gttggcgcat cgcgtgtccg
    15721 cgacctgttc aagcaggcca aggagaaagc gccgtgtatc gtttttatcg atgaaattga
    15781 cgccatcggg cgcgcccggg gcgagaacca gatcaccggc gccaacgacg agcgggagag
    15841 cacgctgaac cagttgctca ccgagatgga cggcttcagc accaacagcg gtgtgatcat
    15901 catggcggcg accaaccggg ccgacatcct tgaccgggcg ttgctgcgtg ccggccgttt
    15961 cgaccgccag atcaacgtgg agctgcctga cctggacgag cgccgcgcta tttttgcggt
    16021 tcataccaag aacctgaaat tcgacaagac cagtgttgac ctggaatggc tggcaaggca
    16081 aacgcccggg ttttcgggag ccgacatagc caacgtggcc aacgaagcag cgctgatcgc
    16141 tgcccgtaac aacaaaaaga tcatcgagaa gcaggatttt atcgacgccg tcgaccggat
    16201 catcggcggg ctggagcgga gaagcaagat catttcgcag gaagagaagc gggtgattgc
    16261 gttccacgag tcaggccatg ctgccgtaag ctggctcctc ccccatgccc atccgctggt
    16321 gaaggtgacc attgtgccgc gcgggaaagc gctcggagcc gcctggtacc tgccggaaga
    16381 gcggcagatc accaccttcg accagatgtt cgacgagatg accgccacgc tgggcggacg
    16441 ggccgctgaa gagatcattt tcggcaaagt gtcgaccggc gccctgaacg acctggagcg
    16501 ggtgaccaag caggcgtaca acatggttgt tttcttcggc ttgaacgaga agctcggcaa
    16561 catcagttac tacgattcaa cggggcagca ggaatacatg ttcaacaagc cctacagcga
    16621 gaaaacggcc gagatcatcg accaggagat cggcaagctt attgaccagg cgtacaaacg
    16681 tgcggttgaa attctcagaa cccataaaga caaactggag gccctggcca gtattttact
    16741 ggaaaaagag gtcattttca ccgaggatct cgagaccatt ttcggcaaga agatcaagca
    16801 tgtcggccac gaagtggtgc tggccgatcc caatggcaac gggcatgttg aggcgcctgc
    16861 tgtggttgaa gaggcgaaac cgaagaaaag ggggcggaag ccaggatcag ttgggcagtt
    16921 ggacagttga cagtatgcag taggcagtag gcagtaaggt gtaatgatat aggtaacctc
    16981 tcccgccagg gagaacagta tggcaggcag ggacgtcaca cagaaacagg gtttccaacc
    17041 ctgaaatttg ggatctttga ataaattgaa aaacgttgat cttcttggat atagtttttg
    17101 gataaatatt cggaaaatta ttccccatat taagtcctta aatatcattc gattgcgtga
    17161 ataaaaaagg ttggaaaccc tgttacaatg ccgaatcggg taattctttg agactccatc
    17221 tcacgccaac cagatggagt ctcaaaccaa aaagttatca aacgcccttg aatttcttat
    17281 ctgattgaac catagttgtt caatccggtt cattcattca ttcattaacc aatcaatcaa
    17341 tccaaatgga atggcgtgct caaataagtt atccccgtag agagagagag agacggggag
    17401 acaaggagac ggggagacaa ggagacgggg agacaaggag acggggagac aaggaga
//
LOCUS       DAOPNH010000032        17395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3182_length_17395_cov_7.690311, whole genome shotgun sequence.
ACCESSION   DAOPNH010000032 DAOPNH010000000
VERSION     DAOPNH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3182_length_17395_cov_7.690311"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKZ74_03020"
     CDS             complement(<1..326)
                     /locus_tag="PKZ74_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91755.1"
                     /translation="MTQIKIASFNLENLFTRPVAMNQDTDAAGAAAIEDHALANTIIN
                     KEVYTDSDKQTLIELSGKYKWHTLNPPKNALVQLQKIRGSLFRKPKNGPLEVVAKGRD
                     SWVGWF"
     gene            complement(568..2022)
                     /locus_tag="PKZ74_03025"
     CDS             complement(568..2022)
                     /locus_tag="PKZ74_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91756.1"
                     /translation="MKKFYLLCLLLILFMITWAQSYIPLPDTNAYWNEIKFYQGQCDP
                     PDFCKYTCYFQGDTILNSLSYHKVYKNDSYEISYSGGLRQDGEKVYYWDRDCNSPVLL
                     YDFGLTVGDSIALACMLCDEEPLYAQVVSVDSVMIGDMSYRKRINFTYGYSYSWIEGI
                     GSVYGLLYPYYSCILCVCYQKLVCFEQDGQVLYLNESDVPCFNFVNPPGDIDPASEWR
                     VDSWTAEPDYSTNSYYRDFIDGDTIINTIRYHKVYQSGYTYYLNYPPGYYYYYEDVFH
                     GLLREEDNRWYTINYNGQDELLYDFTLDVGDTVYSAFTFIFEDPIIVTGVDSVLVGGD
                     YKRRLELNFEEGGGAEYIIEDIGATSGLFENMFFFEWGSELVCFAKNGVSLWGASTEE
                     CDLAVAIGEAETHPNACFISPNPATDHLILTLYGSTGEASFTLINLLGNVVMEGSFTS
                     PSIIKIDLNACSPGIYLAVIKTADTRETFKVVKR"
     gene            complement(2059..2724)
                     /locus_tag="PKZ74_03030"
     CDS             complement(2059..2724)
                     /locus_tag="PKZ74_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91757.1"
                     /translation="MKKTVFTLLLLGIVPFTLQSQFLKGTILKTKPFQDFVAYNPTFG
                     MEKTVGKLFSAELELMYRNRSWNVSGGEWDLGRFYDAGGFKIVIGSRIYFGKTNKNLD
                     QETQKAPFAWYGAIQLGYNLSTTYNVDRKATVSGIYMYTADIDKNWLELNIGIGRQFY
                     LFKTITLDLNLGPSIYFPHQEKTTITKSEDLSEIGTTKTEDFNFGRIIRPNLILSLGY
                     YFK"
     gene            complement(2859..5003)
                     /locus_tag="PKZ74_03035"
     CDS             complement(2859..5003)
                     /locus_tag="PKZ74_03035"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM91758.1"
                     /translation="MRLHLVYFCFLWINVLLCQPTTAQWTWQNPLPTGNTYRGCDFLD
                     TQTGWIVGGYGCILKTTDGGTTWVLQNSGVTTDLYGVYFLDENYGWAVGNGATILHTK
                     NGGNDWMIQHGGSGSGSYINSVYFSDSLHGWTDGTNGGGQYFETTDGGNTWLWRSIPG
                     VSYSWELSSMFFTDSLFFWALTGVDQISHTEDGGNTWITGPSPTGWGEMFFVNHDTGW
                     MTDSHRIYRTTDRALTWEEQLPMQGPAICDLYFTDALHGWAGGEGLLLKTADGGESWE
                     SYETDVLFLAIEFSDMLNGWAVGGWAGELYHTLDGGNTWEDKRAGSDGYITAISFGSP
                     DFGLASYYEDDYHSLLMTTDGGAGWDRLTILEDSIRWNALWVEDDINAWACGDDGTIV
                     HSDDRGQSWVVQREEVPGDWFSELQFLDPLTGFSVGIDAGYTNILLARTYDGGQNWFT
                     LPFHYSASPQGMWFTDPLKGFLVATDMNTDAQVLLTTYSGGESWIITDLPLDDDEDAS
                     SVCFTTSLKGFIGADNCILRTYDGGQNWERYNINGGQIFSLSFPDSLNGWCIGKIYSD
                     RAVLRTNNGGESWAEVPVISDNTLTDLAFTAPDNGWICGYDGLILKWGENNPVSIRDD
                     DTPVYVSVPLISPNPASHWLNIDLTVFPENQKISYRIFNIQGEEIGAGSYQKKGSHDR
                     LTIDIKAINNGIYIVELAMGGERWAGRFVVIK"
     gene            complement(5154..7559)
                     /locus_tag="PKZ74_03040"
     CDS             complement(5154..7559)
                     /locus_tag="PKZ74_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91759.1"
                     /translation="MKRFISLFLFTLACISLHAQIITIKDKENYQPLDLVTLSSQEPA
                     ASAITNIKGQADISAFTGSSLIEIRLLGYEPVKISWADLARAGFHLNLSQSAISLDQV
                     IISATRWSQMTREVPSKITLISAKEVALQNPQTAADLLGASGEIFIQKSQLGGGSPMI
                     RGFATNRVLLVVDGIRMNTAIFRSGNLQNVIALDPFAVEKTEIFFGPGSVIYGSDAIG
                     GVMNFQTLTPRLSVNGEPYISGNAVTRFSSANKEMTGHLDVNVGWKKWAMVTSFSQFH
                     FSDLRMGSNGPDDYLRPNYVIRQDSADVVITNPDPLVQTPTGYDQTSLMQKVRFSPNA
                     NWDLTYGIHYSTTTNVSRYDRLLRTKNGLPRSAEWYYGPQTWMMNTLNIENKATGGIY
                     DRMNIRLAHQLFEESRHDRDRNKATRFNRFEKVNAYSVNIDFTKIIREKHRLFYGLEY
                     ISNKVESTGTDEDITTGESIPGPARYPQSTWASYAAYLTYHYKINEKFNLQAGARYNH
                     YTLDAEFDTTFYPFPFTAANLSDGAITGSVGLVYNPAEKWSFGADISTGFRSPNVDDI
                     GKVFDSEPGAVMVPNPDLSAEYVYNAEIDIARVFGNFMKIDLVAYYTYLDNALVRRDF
                     TLNGQDSIFYDGEMSRVQAMQNAAFATVYGVQAEVEVKLPSGFGFSTQFNYQKGEEEL
                     DDGTKSPLRHAAPWFGKSGLTFSADKLKIDLYAAYSGEVSYESLPPEEQGKDYMYAKD
                     EDGNPYSPSWYTLNFKAMYQISDILSVSGGVENITDVRYRPYSSGITAPGRNLVLSLR
                     ASF"
     gene            complement(7709..8239)
                     /locus_tag="PKZ74_03045"
     CDS             complement(7709..8239)
                     /locus_tag="PKZ74_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91760.1"
                     /translation="MKNILAILLISVILLPISGTYLFFHFKEIQIKKEVNEFIQNKIE
                     EKDIITLRFSDKEIKTRLVWKHSREFIFEGNMYDIVDQGVDGNMTWYRCYRDHKETRL
                     YKEKEKRIARAIGNDPAQKKQSERIKQIFKTSFQIKPFAWNINFPQPSIILFSIFHFP
                     FSIFPPSPPVPPPECA"
     gene            8419..10821
                     /locus_tag="PKZ74_03050"
     CDS             8419..10821
                     /locus_tag="PKZ74_03050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPM91761.1"
                     /translation="MSKENKFYKALQDVFIGAKIEGEGGFINLMRIKSGYYAQIEKVL
                     QADIDHALEQYPHFRDELFDKLFSFFSRYFTQSGSIYFNSTPFHNNIYEKVYTDEKDV
                     ILFWKTQMLYYVKTDRIFKSMPVEFDELKFYFDAGTIENKKANEKRSVIFEMKNVRDD
                     GTIVFSTKYSEKGTVTKLADILKDLKRKNIAITEEQLERAFRVFEKQSEVDFFINKNA
                     RAFLQEQFKLWSFQYFWDGAKEWSGDRVNQLQILKNIAFRIIDFIAQFEDELVKIWNK
                     PKFVKNSNYVITLDKITDAGLVKKLLEHPGIKEQIKEWQELGIVDEQFKTADIQGNKK
                     FEHLPFDTKYFKSLELEILALFDNLDRQLDGWLIKSENYQALNTIRLKFKSKIDLVYI
                     DPPFNTGDDFDYIDNYQDSTWLSILNDRLSLSNSLLKSNGNLYIHLDENANYLGRLIT
                     NHVFGKECFKRELIWDIQVLSGYKVKGAIYNWILGHQNILFYSKAEKSIFNKVIQPHT
                     LKYLESFKQKDERGELFQVAHGRRIYKKDILEKQKPYGDVWNKLSDLIDIQRPFPEVW
                     RELTQTVDENAPFVEVWNDIKSFQQQPTSNERVNFDTQKPEKLLERIIKSSSNAGGII
                     LDFYAGSGTTVCTSLKIEKRKFIGIELAEHFYAEIIPRLKGDLYNGRNTVVAKENGFA
                     GGGFFKYYELEQYEEALNKCIYSDSDLFNQIDEKVYSQYVFLKDEKMLKALEIDYEQN
                     KVKIDLEKLYPGIDAAETLSNLTGKWIKAIRENEVEFADGSKINTKELDYRLIKPLIW
                     WE"
     gene            10823..11656
                     /locus_tag="PKZ74_03055"
     CDS             10823..11656
                     /locus_tag="PKZ74_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPM91762.1"
                     /translation="MEPTKIALFKGKQVRKILHEDEWWFSVVDICGILTESVDPGAYW
                     RKLKQRLKEEGSEVVTNCHGLKLPAPDGKLRETDCADTEGIFRIIQSIPSPKAEPFKR
                     WLARTGFERVKEIENPELATKRTRILYKLKGYSDDWIEKRMRGIAIREELTDEWQKRG
                     AEQKEDYEILTAEISKATFGMTPSEYKNFKGLKRENLRDHMNDFELIFTMLGERATTE
                     ITRNEDSQGMPKLKKDARDGGNIAGDAKRKLEKRLGRKISSRENYLKSPESKKKLRDK
                     S"
     gene            11653..14589
                     /locus_tag="PKZ74_03060"
     CDS             11653..14589
                     /locus_tag="PKZ74_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPM91763.1"
                     /translation="MKLYLQSIAENISFESLPEIWQDFDFGRFSEDKNLFDFQQEALK
                     NTLKALHRFYKDENGEKDKFYGYFDIEENLDFNLEGKEERKTAKFLMEYEKDYPVQNR
                     KISFAYFINRMSYWMATGSGKTLVIVKLIDLLRKLLQNNEIPARDILFLAHRDDLIDQ
                     FKNHVEQFNAFNCDSKINLKSLKEYDQVKHENVLSFGKNEITVFYYRSDLISDEQKEK
                     IISYKNYQNEGKWYIILDEAHKGDKEDSKRQILYTILTRNGFLFNFSATFTDPRDYAT
                     CVFNFNLSRFIEEGYGKHIYISGSKVTAFRDKDDYSLIEKQKIVLKTLILLTYINKYF
                     RKIRQYHQSLYHRPLLLTLVNSVDTEESDLQLFFSEIEKIAANEIPEDFFNTAREELI
                     KEIEDNPSYEFEQLNLNINKELLREIQYQDITENIYNSSFPGRIEVLKIPGNRNEIIF
                     KLQSSDKPFGLIKIGDISGWLKDKLEGYEINESFENESIFKKLNQDDSEINLLMGSRS
                     FYEGWDSNRPNIVLFVNIGMGKDAKKFVLQSVGRGVRIEPLKNKRKRLQNLVSGNEVP
                     ARLFQDLKTSILPVESLYVFGTNAANLKQIIQTLREQRTGRDIGQEFQVNKAREEKQL
                     LIPVYKDSEKNYAEENTVQKFRISKSDFALGRTLYEYLGNKVTLVKFETNLKILEKVA
                     KCFDDQDQYFDFSESRSISDPVLILKRIFDYLGIRNKELERFKELEEEIVHFKKIRFD
                     DGVRYDSILEKIKQVKNYEHKAEQEKDIDKEFDETQDRDIYKQKIRQLELDFLEEATF
                     EELHIKYIPNHYYIPLIISEREKLDYLNHIITVPSEVKFLELLSDYLKNSGILFKRFD
                     WWMFSKLDQSLDEVYIPYYSPRENRISRFKPDFIFWLKKGSDYQILFIDPKGTAHTDG
                     ILKIDGFRQIYEDPLSGHPRMFTYNGFRIAAKLFLMPAQGGIAAVPQDYRKYWFDNFK
                     DLEDKI"
     gene            complement(14596..15318)
                     /locus_tag="PKZ74_03065"
     CDS             complement(14596..15318)
                     /locus_tag="PKZ74_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPM91764.1"
                     /translation="MLAVVSLHSDILDFCRANADEALVRKYSRYFKEGGYDAYGVAYP
                     LMQEKVKSILEMPGVDLSSILELSRLLIPGTKYEEPGFALMLVKAMKKQYTADTFREI
                     GHWFDIGINNWAHTDALCSEIVSVFLQKKIVDYKEIGKWKQGENKFQRRAVPVALIKL
                     MKETKRVEEYVRFIEPLMMDAEREVHQGVGWFLREAWKIDPEPVEEFLLKYKNTAARL
                     IFQYATEKMTPEGKARFRREKR"
     gene            complement(15567..16538)
                     /locus_tag="PKZ74_03070"
     CDS             complement(15567..16538)
                     /locus_tag="PKZ74_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPM91765.1"
                     /translation="MSVLAFTENWDGKFKKISFELISYAHQLAQKMGTHAVALSIGSV
                     SEDELNKLKHYGASKIIKVEDSRIDGLMNKAYAAIIAQVAEKEGANVVVFSHNFTGKA
                     LAPRVAVKLKAGVASGVNAVPDSVEPFVVKKGVFTGKAIGHITIKSPIKVLTLFPNSV
                     ETLTADEPATIESFTPQLTDAHFGTKVVDVKKVTDQILLTDADVVVSGGRGMKGADKW
                     GPLEELAGLLGAALACSRPVSDEGWRSHHEHVGQTGKVIGPNVYIALGISGAIQHLGG
                     VSAAKHIIAINTDKDAPIFEAAEYGMVADVHQVLPQLVEAVKEAKKH"
     gene            complement(16589..17338)
                     /locus_tag="PKZ74_03075"
     CDS             complement(16589..17338)
                     /locus_tag="PKZ74_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPM91766.1"
                     /translation="MKILICLSNVPDTTTKIKFTGDMKSFDAAGVQWIINPWDELALT
                     RALELKETSGATIEKVTVLTVGKADTEPTLRKALAIGADDAARIDADPKDAYQVASQI
                     AGFLKTQPYEIVLCGIESSDYNGSIVGGMLSEFLDCTSVSSVSGLQLENGTIRLNREI
                     DGGQEVVEAGVPFVGIVQKGIAKEPRIPSMRGIMMARSKPLAVVPAFETEELTEFTSY
                     ELPSAHAKCKMIDAENIGGLVEVLKNELKVI"
BASE COUNT         4913 a         3843 c         3934 g         4705 t
ORIGIN      
        1 tcaaaccatc ccacccagct gtcgcggcct ttggcaacca cttccagggg tccgtttttg
       61 ggtttccgga acagcgatcc tcttatcttt tgcaattgaa ccagcgcatt cttcggaggg
      121 ttgagcgtgt gccatttgta tttacccgac aactcaatga gtgtttgttt gtcgctgtca
      181 gtgtaaacct ctttattgat gatggtgttg gccagcgcat gatcttcgat agcggcggct
      241 ccggcggcat cggtgtcctg gttcatcgca accggccggg tgaaaagatt ttccaggttg
      301 aatgaagcaa ttttaatctg tgtcatacgt aagatttttt ggattaattg ataataaggt
      361 tgaattatcc tttagtaaag gattgagggt caaatataag caaaaatata ggaaaagtct
      421 agatatttat gtagattttt ttaattaatg agactccatc tcaatttttc gagatggagt
      481 ctcgatgaaa ggagatggag tctcaccgat ttgagatggc gtctcgatca cctacaatcc
      541 atcttgctcg aagcataaaa gaggcaacta tcttttaacc accttgaagg tttctcttgt
      601 atctgctgtt ttgataaccg ctaagtagat acccggagaa caagcattta aatcaatttt
      661 aataatggat ggagaagtaa atgagccctc catgaccaca tttcccaaaa gattgatcag
      721 ggtgaaagat gcttctccgg tgcttccgta taatgtcagg atcaaatggt cagttgccgg
      781 gttgggtgaa ataaagcacg cgttcggatg ggtttctgct tccccgatag ccacggccag
      841 gtcgcactct tcggtcgaag ctccccacag agaaacgccg ttctttgcaa agcaaaccag
      901 ttccgatccc cattcgaaga agaacatgtt ttcgaacagg ccggaagtgg ccccaatatc
      961 ctctattatg tattccgctc cgccgccttc ttcaaaattc aattccaggc gccttttgta
     1021 atctccgcca accaggaccg aatcgacgcc cgttacgatg atcggatctt caaaaataaa
     1081 agtaaacgcc gagtaaacag tatcgcctac atccagggta aaatcgtaca gcaactcatc
     1141 ctggccattg taattaattg tataccagcg gttatcttct tccctcaaca gcccatgaaa
     1201 tacatcttca taataataat aatatcccgg aggataattc agataatagg tatatcctga
     1261 ttggtaaacc ttatgatatc ttattgtatt gatgatggta tccccatcta taaaatcccg
     1321 gtagtatgaa ttcgtcgaat aatccggttc ggccgtccac gaatccacgc gccattcgga
     1381 ggccggatcg atgtctcccg gtggattgac gaaattgaaa cagggaacat ccgattcatt
     1441 caggtaaagg acctggccat cctgctcgaa gcagaccagt ttctggtaac agacacataa
     1501 aatgcaggaa taatacggat acagcaagcc gtacacgctt ccgatccctt cgatccacga
     1561 ataggaataa ccataggtga aattgatcct tttccggtag ctcatatccc cgatcatcac
     1621 cgagtcgacg gagactacct gggcatacag cggttcctca tcgcaaagca tgcaggccag
     1681 ggctatggaa tccccgacgg tcagcccgaa atcatataaa agcaccggag aattgcagtc
     1741 ccgatcccag taatagactt tttcaccatc ctgccttaat cctccggaat aggaaatttc
     1801 ataactgtca tttttataaa ctttatgata agataaagaa ttgagaattg tatccccctg
     1861 gaaatagcag gtgtacttgc aaaaatccgg cgggtcgcac tggccctggt agaattttat
     1921 ctcattccaa taagcattgg tatccggtaa agggatatac gattgtgccc aggtaatcat
     1981 gaacagtatg agaagcaggc ataatagata aaactttttc atggctattt tgttttgatg
     2041 cgggattggc cgctgttatt atttaaaata atatcctaat gataggatta gatttggcct
     2101 gataattcgt ccaaaattga aatcctcggt tttcgtcgtc ccgatttcgc tcaaatcttc
     2161 actttttgta atggttgttt tttcctggtg tggaaaataa atggagggcc ccaggttaag
     2221 atccagggtt attgttttga ataagtaaaa ttgccgtccg ataccgatat tcagctccag
     2281 ccagttttta tctatgtcag ccgtatacat ataaatgcct gaaactgtag ctttccggtc
     2341 gacattatac gtagtgctga ggttatagcc caactggatg gctccatacc aggcaaacgg
     2401 agctttttga gtttcttgat ccaggttctt gtttgttttt ccaaaataga tccttgatcc
     2461 gatcacaatt ttgaatccgc cggcatcgta aaagcggcca agatcccatt ctccaccgga
     2521 tacattccag ctcctgttac ggtacataag ttcaagctct gcgctgaata acttaccaac
     2581 cgttttttcc attccaaagg tcggattata agcgacgaaa tcctgaaagg gttttgtttt
     2641 aaggattgtc ccttttaaga attgagattg taaggtaaat ggaactattc ccagcaacag
     2701 tagtgtgaat acggtctttt tcataatcta cttataaagc tatatatcaa tcatttgttc
     2761 cgcctggcat atccgtgcac tgtgagactc catctcaatt tttcgagatg gagtctcgag
     2821 tgaaagcaga tggcatctca ccggttcgag atggagtctc attttataac aacaaacctc
     2881 ccagcccatc tttctccgcc catggccagt tcgacaatat atattccatt attaattgct
     2941 ttaatatcaa tcgttaaccg gtcatggctc cctttttttt gatatgatcc cgccccgatc
     3001 tcctcaccct gaatattaaa gattctatat gatattttct ggttttccgg gaaaacggta
     3061 aggtcaatgt tcagccaatg gcttgccgga ttcggggaaa ttagcggaac ggaaacataa
     3121 acaggcgtat catcatccct gatagagacc gggttgtttt cgccccattt caggatcagg
     3181 ccatcgtatc cgcagatcca tccgttgtca ggcgctgtga aagctaagtc ggtcaaggta
     3241 ttgtcggaaa tcacagggac ctcagcccag ctttcaccgc cattgtttgt gcgcaatact
     3301 gctctatcag aataaatttt gccaatacac cagccgttca gggaatcggg aaaggaaagg
     3361 gaaaatatct ggcctccatt gatattgtat ctctcccagt tctggcctcc gtcgtaggtt
     3421 cgcagaatgc aattgtcggc tccgataaag ccttttaatg aagtggtaaa gcaaaccgac
     3481 gaagcatcct catcatcatc gagcggcagg tcggtgatga tccagctttc gccaccggaa
     3541 taggttgtca ggagaacctg tgcgtccgtg ttcatgtcgg tggctacaag aaatcccttt
     3601 aacggatcgg taaaccacat accctgtggc gaagccgaat aatggaacgg tagagtaaac
     3661 cagttctgcc ctccgtcgta agtcctggca agaaggatat tggtgtaccc ggcatcaatc
     3721 cctacagaaa atccggtcag gggatccagg aactgcagtt cagaaaacca gtctccaggc
     3781 acctcttccc gctgcacaac ccaggattgc ccacgatcgt ccgaatgaac aatggttccg
     3841 tcgtcgccgc aggcccaggc attgatatca tcctccaccc aaagagcatt ccaacgtatg
     3901 gaatcttcca aaatcgtcag gcggtcccat ccggccccac cgtctgttgt catcaacagg
     3961 gagtggtaat catcttcgta atagcttgcc aaaccaaagt cgggactccc gaaactgatg
     4021 gcagtgatat acccatcact gcctgcacgc ttatcttccc atgtatttcc tccgtcaagt
     4081 gtatgatata attctcctgc ccaacctcct acggcccatc cgttcagcat atctgaaaac
     4141 tcaatagcca ggaagagcac atctgtttcg taactctccc aggattctcc gccatccgct
     4201 gtttttagta ataagccctc accacctgcc catccatgca gggcgtcagt aaaatagaga
     4261 tcgcaaatag ccggcccctg catcggcaac tgttcttccc aggtcagggc tctgtcggtg
     4321 gtgcggtaaa tccggtgact gtcggtcatc catcctgtat catgatttac gaaaaacatc
     4381 tctccccagc cggttggcga tggccccgtt atccaggtat ttccgccgtc ttccgtgtgc
     4441 gaaatctggt cgactcccgt tagggcccag aagaaaaggg aatcggtaaa aaacatggac
     4501 gaaagttccc aggaatacga aacgcccgga atgcttctcc acagccaggt attaccccca
     4561 tcggttgttt caaaatactg gcctccaccg ttggttccat cagtccatcc gtgtaatgag
     4621 tcagaaaaat agaccgaatt gatataagaa ccggagccgg aaccgccgtg ctggatcatc
     4681 caatcgtttc cgccattctt ggtatgcagg atcgtggcgc cgtttccgac tgcccagcca
     4741 tagttttcat ccaggaaata tacgccatac agatcggtag tcacgccgct gttctgcagc
     4801 acccaggtgg tcccgccatc ggtagttttc aggatacagc catagccgcc gacgatccag
     4861 cccgtctggg tatcgaggaa gtcgcaccca cgatatgtat tgcccgtcgg cagcgggttt
     4921 tgccaggtcc attgggctgt tgttggttgg cagagtagta cattgatcca tagaaaacag
     4981 aaataaacca gatggagtct cataatttca ccgataggca aatttctaat gccttaaaat
     5041 tatgaaaatt acatgataga tcgaatggag ttaaatattt ttttaatgag actccatctc
     5101 accatttcga gatggagtct cgtggaaatg agatggagtc tcattttgtt ccatcagaaa
     5161 ctcgcccgga gcgacaacac caggttccgc cccggcgccg tgatcccgct gctgtatggc
     5221 ctgtacctga catccgtgat gttctccact ccgccgctga ccgataaaat gtcgctgatc
     5281 tggtacatgg ccttgaaatt caaggtgtac catgatggcg agtagggatt gccatcttcg
     5341 tctttcgcat acatgtagtc tttgccctgc tcctccggcg gcaggctctc ataactgact
     5401 tcgccgctgt atgccgcata caggtctatc ttcagcttgt cggccgaaaa tgtcagcccc
     5461 gatttgccaa accagggggc tgcatgacgc agcgggctct tcgtgccgtc gtcaagctcc
     5521 tcttcccctt tctggtagtt gaactgtgtc gaaaatccga accccgacgg gagtttcacc
     5581 tccacctcag cctggactcc atatacggtg gcaaaagccg cattctgcat cgcctgcacc
     5641 cggctcatct cgccgtcata aaagatgctg tcctgcccgt tcagcgtaaa atcccgcctg
     5701 acaagggcat tgtcgagata ggtgtagtag gccaccaggt cgatcttcat gaaattaccg
     5761 aacacccggg caatatcaat ctctgcattg tacacatact cggcgctcag atcgggattg
     5821 ggcaccatca ccgcccctgg ttctgagtca aatacttttc cgatgtcgtc cacattgggc
     5881 gaacggaacc ctgtggaaat gtcggccccg aacgaccatt tctcggccgg gttgtacacc
     5941 aggcccacgc ttccggtaat ggccccgtcg ctcagatttg cagccgtaaa cgggaagggg
     6001 taaaaggtcg tatcaaattc cgcatccagc gtgtagtgat tgtaccgtgc cccggcctgc
     6061 agattaaact tttcgttgat tttgtaatgg tacgtcaggt aagcagcgta cgaagcccag
     6121 gtcgactgcg ggtaacgggc cgggcccggg attgattcac cggttgtgat atcctcgtcg
     6181 gtcccggtcg attcaacttt gttggaaatg tattccaggc cgtagaaaag ccggtgtttt
     6241 tcccgtatta tcttcgtaaa gtcgatattg acggaataag cattcacctt ttcaaagcga
     6301 ttgaatcttg tagctttgtt ccggtcgcgg tcatgacggc tctcctcgaa caactggtgc
     6361 gccaggcgga tattcatccg gtcgtaaatt ccaccggtag ccttgttctc gatgttcagc
     6421 gtgttcatca tccaggtctg cgggccgtag taccattcgg cgctccgggg aaggccgttt
     6481 ttggtcctga gcaaacggtc gtaacgcgac acgttggtgg ttgtcgaata atgaatcccg
     6541 tatgtcagat cccagttggc attcggcgaa aacctgacct tctgcatcag gctggtctgg
     6601 tcgtatccgg taggggtctg caccagcgga tcgggattgg tgataaccac atcagcgctg
     6661 tcctggcgga tgacgtaatt cggcctgaga taatcgtcgg ggccattcga tcccatcctg
     6721 agatcagaga aatgaaattg agaaaagctt gtgaccatgg cccacttttt ccatcccacg
     6781 ttgacatcta aatgacctgt catttcttta ttggcggatg agaagcgagt tacggcattg
     6841 ccagatatat aaggctcacc gttcacggag agacggggag tgagggtctg gaagttcatg
     6901 accccgccga tggcatcgct gccatagatc accgaaccgg ggccgaagaa gatctcggtc
     6961 ttttccacgg cgaacggatc gagagcgatg acgttctgca ggttcccgct gcggaaaatc
     7021 gccgtattca tgcggattcc atcgacgacc agcagaacgc ggttggtggc aaaaccgcgg
     7081 atcatcgggc tgccccctcc gagctggctt ttctggatga aaatttcacc ggaggcgccc
     7141 agcaggtcgg cggccgtttg gggattctgc agggcaactt ccttggccga aatcaacgtg
     7201 atctttgagg gcacctcgcg ggtcatctgg ctccagcgcg tagccgagat gatcacctgg
     7261 tcgagggata tggcagactg gctgaggttg aggtgaaagc ctgctcttgc aaggtcggcc
     7321 cagcttattt tcaccggttc gtatcccagc aaccggatct cgatcaggga tgatcccgtg
     7381 aatgcggaaa tatcagcctg tcctttaata ttggtgatgg ccgatgctgc cggttcctgg
     7441 ctggacaggg tgaccaggtc cagcggctgg taattctctt tgtcttttat ggtaatgatt
     7501 tgtgcatgaa gagatatgca tgccagggtg aataaaaata gagatataaa tcttttcatc
     7561 tttcagattt tatattttgt cataataaat attaataacg tacacacaac ttgtcccgat
     7621 ttttcgggaa agggcaaaac agtaaccaca tagaatctat tgtgtatcct atgtgttcta
     7681 tctgcctatg tggttaaaag aatgttatct atgcacattc aggcggcggg actggcggag
     7741 atggagggaa aatggaaaat ggaaaatgga agatggaaaa tagaataatt gagggttggg
     7801 ggaagttgat gttccaggcg aacggcttga tctggaaaga agtcttaaag atctgcttta
     7861 tcctttcgga ttgttttttc tgagccggat cgttgcctat ggcccgggct atccgctttt
     7921 ctttctcctt ataaagccgg gtctccttat ggtcgcggta gcatctgtac caggtcatat
     7981 ttccatcaac accctgatca acaatgtcgt acatgtttcc ttcaaaaata aactcccggg
     8041 aatgtttcca tacgagcctg gtctttatct ctttatcaga aaaccttaag gtaatgatat
     8101 ctttttcttc tattttattt tgaatgaatt cattaacctc tttttttatt tgaatttctt
     8161 taaaatgaaa aaagagataa gtccctgaga tggggaggag gataacggat atcagcaata
     8221 tagcaaggat attcttcaaa agatttcaat caagagtgta aaattatgta aaatgatgga
     8281 ttagaaggct aatttgttat ttgtaatgga aaaggatttt caccacagat ttcccggagt
     8341 aatcacagat ttggattata tttgaaggga atttagtcta taacatattc ttttcatagc
     8401 atttatattc aattatttat gagtaaagaa aataagtttt acaaagcgct tcaggatgtt
     8461 tttataggag ctaaaattga gggtgagggt ggattcatta atctgatgcg cattaaatcc
     8521 ggttattatg cacagattga aaaagtgctc caggctgata ttgaccatgc tttagaacaa
     8581 taccctcatt ttcgtgatga gcttttcgat aaattatttt cttttttcag ccgctatttt
     8641 acccaaagtg gctctatcta tttcaattcc acgcctttcc acaataatat ctacgaaaaa
     8701 gtttacaccg atgagaaaga cgtgatcctg ttctggaaaa cccagatgct ctattatgta
     8761 aagacggacc ggatttttaa aagcatgccc gttgaatttg atgaactgaa attttatttc
     8821 gatgccggaa cgattgaaaa taagaaagcg aatgaaaaaa ggtcggtcat ttttgaaatg
     8881 aagaatgtca gggatgacgg gacaatcgta tttagtacga aatactctga gaaagggaca
     8941 gttacaaagc ttgctgatat cttaaaagac ctgaaaagga aaaatattgc cattaccgag
     9001 gaacagcttg aaagggcttt ccgggtattt gagaagcaaa gcgaggtgga tttctttatc
     9061 aataagaatg cccgggcatt cctgcaggag cagttcaagc tctggagctt ccagtatttc
     9121 tgggatggcg cgaaagagtg gtcaggcgac agggtaaacc agcttcagat cctgaaaaac
     9181 atcgcgttca gaatcattga tttcattgcc cagtttgaag atgaactggt gaagatatgg
     9241 aacaagccca aattcgttaa aaacagcaat tacgttatta cccttgataa aataaccgat
     9301 gccggcctgg ttaaaaaact gctggaacat ccgggaataa aggaacaaat taaggaatgg
     9361 caggaattag gtattgtgga tgaacaattc aaaaccgctg atatacaggg gaacaagaaa
     9421 tttgaacacc tgcctttcga tacgaagtat tttaaaagcc ttgagcttga aatcctggca
     9481 ttattcgaca atctcgaccg gcaactcgac ggctggctta tcaaaagcga aaactaccag
     9541 gcactgaata ccattcgact gaaattcaaa agcaagattg atttagttta tatagatcct
     9601 ccatttaata caggagatga ttttgattat attgataatt atcaagatag cacatggttg
     9661 tctattttaa atgacaggct tagtctttca aatagcttgt taaaatcgaa tggaaatcta
     9721 tacattcatc ttgatgagaa tgctaattat cttggacgtc taataactaa ccatgttttt
     9781 ggtaaagaat gttttaaaag ggaattaatt tgggatattc aggtcctttc aggatataaa
     9841 gttaaaggag ctatttataa ttggattcta ggccaccaaa atatactttt ttattcaaaa
     9901 gctgagaaaa gcatttttaa taaagtaatt caacctcata cacttaagta tcttgaaagc
     9961 ttcaaacaaa aagatgaaag aggtgaatta ttccaagtgg ctcatggtag aagaatctat
    10021 aaaaaagaca tacttgaaaa acagaaacca tatggtgatg tgtggaataa gcttagtgat
    10081 ctgattgata ttcaaagacc ttttccagaa gtatggagag agttaactca aacagttgat
    10141 gaaaatgcac cttttgttga agtttggaat gacattaaat catttcaaca acaacctaca
    10201 tctaatgagc gagttaattt tgatacacaa aagccagaaa aattattaga aagaataatt
    10261 aagtcatctt caaatgctgg agggattatt ttggattttt atgctggtag tggaacaaca
    10321 gtctgtacat cgttgaaaat tgaaaagaga aagttcatag gaattgagct agcagaacat
    10381 ttttatgctg agataatacc gagattaaag ggggatttat ataatggtag aaatactgtt
    10441 gttgcaaaag aaaatggatt tgcaggcggc ggtttcttca aatactacga gctcgagcaa
    10501 tacgaagagg ctttaaacaa atgcatttac agcgattcgg atttgtttaa tcaaatagat
    10561 gaaaaggtat acagccagta tgttttcctg aaggatgaaa agatgcttaa ggcattagag
    10621 attgattatg agcagaataa agtaaaaatc gacctagaaa agctttaccc gggcatcgat
    10681 gcagcggaga cattatcgaa tcttaccggc aaatggataa aagccatccg ggaaaatgaa
    10741 gtggaatttg ccgatggaag caagatcaac acgaaagagc tggattaccg gctgatcaaa
    10801 cctttaatct ggtgggaata agatggaacc gacgaagatt gcattattta aaggaaaaca
    10861 ggtgcgtaag atcctgcatg aagatgaatg gtggttctcc gtggtagata tctgtgggat
    10921 tctaactgaa agtgtcgatc ccggcgcata ctggcgaaaa ctgaaacaac gtcttaagga
    10981 ggagggaagt gaagtcgtga caaactgtca cgggttgaaa ttgcctgctc ccgacggaaa
    11041 gctccgtgaa acggactgtg cagacaccga aggcatattc aggatcatcc aatccatccc
    11101 atcacccaaa gcagagccat tcaaacgctg gttagccaga accggtttcg aacgggtcaa
    11161 agagatcgaa aatcccgagc tggctactaa aagaacgcgg atactataca aactgaaagg
    11221 ctacagcgac gactggattg aaaagcgtat gaggggaatt gccatccggg aggaactcac
    11281 ggatgaatgg cagaaacgcg gcgctgaaca aaaagaggat tatgagattt taactgcaga
    11341 aatctcaaaa gctaccttcg gaatgactcc ttcggaatat aaaaacttca aaggcctgaa
    11401 gcgggagaac ctgagggatc acatgaatga ttttgaactg atatttacca tgttgggcga
    11461 acgcgcaacc actgaaatta ccagaaatga agactcccag gggatgccaa agttaaagaa
    11521 agatgcgcgg gatgggggca atattgccgg cgatgcaaaa cggaagcttg aaaaacgcct
    11581 tggcaggaaa atatcttcca gggaaaacta cctgaaatca cctgagagta aaaagaaatt
    11641 gcgggataaa tcatgaaact ttacctacaa tccatagccg aaaatatatc tttcgagagc
    11701 cttccggaaa tctggcagga ctttgatttt ggccggttct cggaagataa gaacctattc
    11761 gactttcagc aggaagcctt gaaaaacacc ctgaaagcgc ttcatcgttt ttataaagat
    11821 gaaaacgggg aaaaagataa attctacggc tactttgata ttgaagagaa tcttgatttt
    11881 aacctggaag gcaaagagga aagaaaaaca gcaaaatttc tgatggaata tgagaaagat
    11941 tatccggtac aaaacaggaa aatctcattt gcatacttca ttaaccggat gagttactgg
    12001 atggccactg gttcaggaaa gacgctggtc attgtcaagc tcatagattt acttcgtaaa
    12061 ttattgcaga acaatgaaat accggcaagg gatattttat ttcttgccca ccgggatgac
    12121 ctgatcgacc agtttaaaaa ccatgtcgag cagttcaatg catttaattg tgatagcaaa
    12181 atcaacctga aaagccttaa ggagtacgac caggttaagc atgaaaatgt tttatctttt
    12241 ggtaaaaatg aaatcactgt attttattac cgctccgacc tgatctcgga tgagcagaag
    12301 gagaagatca tcagttataa gaattaccaa aacgaaggca agtggtatat catcctggat
    12361 gaagcccaca aaggagataa agaggacagt aaaaggcaga tactttatac catcttaaca
    12421 cggaacggat ttttattcaa cttttcggca acattcacgg atccacgaga ttatgcaaca
    12481 tgtgttttta atttcaatct ttcgagattt atcgaagagg gctatgggaa acatatttat
    12541 atttccggtt ccaaggttac agcattccgc gataaggacg attattcgct cattgaaaaa
    12601 cagaagattg tcctcaaaac cttaatcctg ctaacctaca tcaataaata tttcaggaag
    12661 atcaggcagt atcatcaatc gctgtatcac aggccattgt tgttgacatt agtcaattca
    12721 gtcgatacgg aagaatcgga tttgcaattg ttcttttcgg aaattgagaa aatcgctgct
    12781 aatgaaattc ctgaagattt ttttaacaca gccagagagg agttaatcaa ggaaattgaa
    12841 gataatccct cttatgaatt cgaacagctg aatcttaata ttaacaagga gctcttaagg
    12901 gagattcaat accaggatat taccgagaat atttacaatt cgtcatttcc gggtaggatt
    12961 gaggttttaa aaattcccgg taacagaaat gagatcattt tcaagcttca gtcatcggat
    13021 aaacccttcg gactcattaa aatcggggat atttcgggct ggctgaaaga caagcttgaa
    13081 ggatacgaaa tcaacgaaag ctttgaaaat gaaagtattt ttaaaaagct taaccaggat
    13141 gactcggaaa tcaatttgtt gatgggttcc agatcgttct atgaaggatg ggactcaaac
    13201 aggccgaata tcgttctttt tgtcaatatt ggtatgggca aagatgcaaa aaagttcgtt
    13261 ttgcaatcag tgggccgggg agtaaggatc gagcctttga agaacaagcg aaaaagactc
    13321 caaaatcttg tttccggaaa cgaggtaccg gcaaggcttt tccaggattt gaaaactagc
    13381 atattgccgg ttgagagcct gtacgtgttt ggaaccaatg ctgcaaacct gaagcaaata
    13441 attcagacgc ttagagaaca aagaacaggc cgtgacatcg gtcaggaatt tcaggtcaat
    13501 aaagccaggg aagaaaagca attattgatc cctgtttata aagattcgga aaaaaactac
    13561 gctgaggaga atactgttca gaagtttcgg atcagtaaaa gtgactttgc cctcggaagg
    13621 acgctttatg aatatcttgg aaataaggta actctggtta agtttgaaac caatctgaag
    13681 atcctggaga aagttgcgaa atgttttgac gatcaggatc aatactttga ttttagcgag
    13741 tcacggtcaa tttctgatcc tgttctgatt ctaaaacgaa ttttcgatta cctgggcatt
    13801 cgaaataagg aactggagcg gtttaaagag ctggaggagg agatcgttca ttttaaaaag
    13861 atcaggttcg atgatggcgt cagatatgat tcaatcctgg agaagatcaa acaggtcaag
    13921 aattatgaac acaaagccga acaagaaaaa gacatagaca aagaatttga tgaaacacag
    13981 gatcgggaca tctataagca aaaaatcagg cagcttgaat tagattttct ggaggaggct
    14041 acatttgagg aactgcacat taagtatatt ccgaatcact attatattcc gttgattatt
    14101 tcggagaggg aaaagctgga ttacttaaat cacattataa cagttcccag tgaagtaaaa
    14161 ttccttgaat tattaagcga ttacctgaaa aatagcggca ttttatttaa aagatttgat
    14221 tggtggatgt tttcaaagct ggatcaaagt cttgatgagg tatatattcc ttattatagc
    14281 cctagggaaa acaggatttc aagattcaaa cccgatttta tattctggtt aaaaaaagga
    14341 tctgattacc agatactatt tatcgatcca aaagggactg cccatacgga tggaatatta
    14401 aaaattgatg gattcaggca gatttatgaa gatccacttt caggtcatcc caggatgttt
    14461 acctacaatg gttttcggat tgcagcaaaa ctgtttctaa tgcctgcaca gggtggtatt
    14521 gccgccgtac ctcaggatta ccgtaaatat tggtttgata atttcaaaga ccttgaggat
    14581 aaaatttgat gactattacc ttttctcccg tcggaacctc gccttcccct ccggcgtcat
    14641 tttctccgta gcatactgaa agatcagtcg ggcggcagtg tttttgtatt tcagtaaaaa
    14701 ttcttcaaca ggttcaggat caatcttcca cgcctctctc aggaaccagc caaccccctg
    14761 gtgcacctcc cgctctgcat ccatcatcag cggctcgata aaacgaacat attcctcaac
    14821 tctcttcgtc tctttcatca gtttgatcaa tgcaaccggc accgccctcc gctgaaattt
    14881 attctccccc tgtttccatt ttccaatttc tttataatcc acgatcttct tctgcaggaa
    14941 aaccgacacg atctccgaac acagcgcatc ggtatgggcc cagttgttga tcccgatgtc
    15001 gaaccagtgg ccgatctcgc gaaaagtatc ggcagtatat tgctttttca tcgccttgac
    15061 cagcatcagg gcaaagcccg gctcttcgta tttggttccg gggatcagca accggctgag
    15121 ctccaggata gaactgagat cgaccccggg catttccagg atggatttca ccttctcctg
    15181 catcagcggg taggccacgc cataagcgtc atatcctcct tctttgaaat agcgggagta
    15241 ctttctcacc agtgcctcat cggcattggc ccggcagaaa tcgaggatat cagaatggag
    15301 agaaacaaca gccagcataa gttttaaatc ctaagttttg tgttttatct tctgtgttat
    15361 agtcctccgg tgaatccccc cggtatctct ctttcaaaca ataaatcaca aaagcaaaac
    15421 aaaagaaatc aaaacatgat aatgccataa tatcaaaaca tcacaatatc acaatatcac
    15481 aatatcacaa tatcacaaca tcacaacatc acaatatcac aatatcacaa catcacaata
    15541 tcacaacatc aaaatatcaa aatatcttaa tgtttcttcg cttctttcac tgcttccacc
    15601 agctgcggca gcacctggtg aacatcggcc accatgccgt attcggcggc ttcgaagatg
    15661 ggagcgtctt tatcggtatt gatggcgata atgtgtttgg cagcgctgac gcctcccagg
    15721 tgctggatag cgcccgagat gcccagggcg atgtagacgt tagggccgat gactttgcct
    15781 gtctggccca catgttcatg gtgcgagcgc cagccttcgt cggatacggg cctggagcaa
    15841 gccaatgcag ctccaagcag ccctgcgagc tcttccagcg gcccccattt gtctgcgcct
    15901 ttcattcccc tgccgcctga aacaacgacg tcggcatcgg tcagcaggat ctgatcggtt
    15961 acttttttta catcgactac tttggttccg aaatgagcgt cggtcagctg cggagtgaac
    16021 gattcgatcg ttgcaggctc gtcggccgtg agggtttcca cgctgttggg gaaaagtgtc
    16081 agcactttaa ttggcgattt tatcgtgatg tggccgatag ctttcccggt aaatacccct
    16141 ttttttacta caaacggttc gacagaatcg ggaacggcat tcacgccgga tgccacgccg
    16201 gctttcagtt tcacggccac tctcggagca agggccttgc cggtaaaatt atgtgagaac
    16261 accacaacat ttgccccttc cttttcggcc acctgggcga tgatggcggc atatgccttg
    16321 ttcatcaggc cgtcgatccg gctgtcttcc actttgatga tctttgaagc gccgtaatgc
    16381 ttcagcttat tcagctcatc ttccgaaaca ctgccgatcg ataacgcgac cgcatgcgtc
    16441 cccatttttt gtgcaagctg atgtgcatat gagatcagtt caaagctgat cttcttgaac
    16501 ttaccgtccc aattctctgt aaaagctaat actgacatat taagaattct aaattataaa
    16561 ctttgaattt tgaattttga actttgaact aaattacttt cagttcattt ttcagcacct
    16621 ccaccagtcc gccgatattt tccgcatcga tcattttgca tttggcgtgc gcggagggca
    16681 gttcatagct ggtgaactcg gtcagctctt cggtctcgaa tgccggaacc acagccagcg
    16741 gtttcgaccg tgccatcatg ataccgcgca tcgaagggat acggggctct ttggcaattc
    16801 ctttctgaac gatgccaacg aatggaacgc cggcctccac cacctcctgt ccgccgtcga
    16861 tttcgcggtt gagccggatg gtaccgtttt ccagttgcaa tccggaaaca gaagaaaccg
    16921 aggtacagtc gaggaattcc gacagcatcc cgccgacgat gctcccgttg taatcgctgg
    16981 attcgattcc gcagaggacg atctcatacg gctgtgtttt caggaagccg gcgatctggg
    17041 aagctacctg gtaggcgtct ttcggatcgg cgtcgatcct ggccgcgtcg tcggcgccga
    17101 tggccagcgc tttgcgcagg gtcggctcgg tatcggcttt gccaacggtc agcacggtca
    17161 ctttttcgat ggtggcgccc gaggtttcct tcagttccag ggcgcgggtc agcgccagtt
    17221 cgtcccacgg gttaatgatc cattgcacac cggcagcatc aaaacttttc atatcgccgg
    17281 taaatttgat ttttgtcgtg gtatcaggga cgttggagag gcagatgagg attttcatgg
    17341 gttctattgt tctattgttc tattgttcta ttgttctatt gttctattgt tctat
//
LOCUS       DAOPNH010000033        16661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3387_length_16661_cov_6.198242, whole genome shotgun sequence.
ACCESSION   DAOPNH010000033 DAOPNH010000000
VERSION     DAOPNH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_16661_cov_6.198242"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PKZ74_03080"
     CDS             <1..1792
                     /locus_tag="PKZ74_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91767.1"
                     /translation="NRRNYFSVQYLNSSRKYHIYNFEGEKLPNISLYDQYANLLFHSS
                     TNSNHTWEAGPSIEAYHSYRPPLTAGGSDAEYHAQKVRFEYKSVIARALMLNMKTGIS
                     RINHIETDTLTYQRYDFHLMGGYNFGRGYSISFSYDYGPMVNNGLYQFAGDIKNHSFN
                     ISPSMMKSYFKERINLTIFSNFIYRMDLGYISVNVNPKIEAYLFNDWYAVASGTYHFT
                     QQEYTDQTTRNSYVYAEVSIRKRFGKSDFNKWQKHTRRLKVVMFKDDNGNGTKEDNEQ
                     GIPFVKTRLKLTNTDNPYVSTQFPVDIILLSNKAGAVNYNKLPRGFYELTVTPLTDMK
                     EYFYVNRSAEKLELIKNATYYVPFQKASKISGKVTVQRQKFIKTGEESLDLTNIKVTA
                     YNKQGNSYSSFTLSDGSFTIYVPDNNSYYLRMGNVFGSNFRITQNDINVDVAGGKVPE
                     VVFNVVENSRQVKFKGAKPAVTDTAQAEPLKIKVLHGKFYENSKEAAVNKNAVPVFNI
                     PEAAVQEEPIIPGNFYVVTGSDSTRTQAVKIKRIMDENGLDARLGYNESEGMYYIFTG
                     YYPNKGDAKDELERLKAAGMADVEIIKFQE"
     gene            2167..2475
                     /locus_tag="PKZ74_03085"
     CDS             2167..2475
                     /locus_tag="PKZ74_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91768.1"
                     /translation="MNPAQNIVIALFLTIQLGIGENRIYIKPSVFTLPEIIFTNQANY
                     ELSKKNKIPRTDPPAIRIYHKNNVHHPEKSFQVKVTMPDLVAVFHRGNGLDNYNKKIK
                     "
     gene            2472..3338
                     /locus_tag="PKZ74_03090"
     CDS             2472..3338
                     /locus_tag="PKZ74_03090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM91769.1"
                     /translation="MKTRQTALITGATSGIGKAFAVCYAGMGYDLVVTGRRMDRLEQL
                     CSELSREYGIRAEAVKAELSEEQDMERLLKVIIRRDNIGILVNNAGFGSGVDFYQNSL
                     EEHLKMLDVHVKASIKLIYAVLPQMRMRKEGVIINVSSLGAFMPAPGSSMYSATKLFL
                     ASFSESLNMEVRRHGIKVRCVCPGFTHTEFHERRAQGKIRAGGKLMWMEADDVVRKTI
                     RSLKKNKIVVVPGIINRLLIAVTSIIPRRLYYHLMERSGKAKVGPGFIDRMKQLVEKP
                     VSFFFTTHTDQV"
     gene            complement(3237..4244)
                     /locus_tag="PKZ74_03095"
     CDS             complement(3237..4244)
                     /locus_tag="PKZ74_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM91770.1"
                     /translation="MTETPGLQQFFFQQIREKIPDNMSFVHEIAELLEISYDSAYRRI
                     RGEKELSLEELVKLSKKFNLSLDALAGLDPNHVVFRKFALHPGDFNIKDWLLQIRDDI
                     RRIQTARNPEIIYAAKDPPVFHYFHFPEIAAFKFFFWEKTIFNFPEYENKLFRLDDVN
                     PEIVDIGNDILRAAIKIPTTEIWNEDTFRILMRQIEYYWISGYFEKKEDILNLVDKLE
                     KWVLHIRKQAEMGFKFHYGMPAEGIPNSFMMYENEVVLNDNSIWVRVGDHMATYLTFD
                     VISLLVTQDKAFCTNVEQHMRQLMKKSNLISVSGEKERNRFFNKLLHTIDKARTDLGL
                     S"
     gene            complement(4371..5321)
                     /locus_tag="PKZ74_03100"
     CDS             complement(4371..5321)
                     /locus_tag="PKZ74_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HPM91771.1"
                     /translation="MIEKRIIAIIGATGAQGGGLADAILSDSQSEFSVRAITRNINAD
                     KAKALADRGAEVVSADLDDTESLEKAFNGAYGVFGVTNFWEHFSPEKETAQAKNIADA
                     ALAAGIKHVIWSSLEDTRDFIPLDDNRMPTLMGKYKVPHFDSKGEAEKFFQQPGLTST
                     ILRTSFYWDNYIYFGLGPKKGPDGVLAITFPMGDKKLPGIVAVDIGKCAYGIFKAGEK
                     YKNKTIGIAGEHLTGAQMAASFTRVIGAEVKYNDVPADVYRSFGFPGADEMGNMFQFK
                     RDFEADFVGGSNLEVARSINPSLQSFDQWLDQNAGKISIE"
     gene            5536..6351
                     /locus_tag="PKZ74_03105"
     CDS             5536..6351
                     /locus_tag="PKZ74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91772.1"
                     /translation="MILRPRLKAEIERSLSVSPVVALLGPRQCGKTTISRLIAKNHKA
                     VIFDLEDPVDYQQLSAAPLITLQSLNGLVIIDKVQKMPELTGILRVLADRSNSPAKFL
                     ILGSASPVMMKNASESLAGRVSFIDMSGFNLEEIGAEHLRMLWTRGGFPRSFLGKDER
                     ISFQWRQDFIRTFLERDIPQLGISIPAVSLRRFWTMLAHYHGNIWNGSEFARSIGTTE
                     PTARRYLDILSGAFVVNQLQPWHENLKKRQVKSPKVYIRDSVLLLGHSRRGRN"
     gene            6584..9007
                     /locus_tag="PKZ74_03110"
     CDS             6584..9007
                     /locus_tag="PKZ74_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91773.1"
                     /translation="MNAIRRFIKTVPKWAFVVVLVVIFVQTANMFYNARMPVSGEREN
                     NDNTNGYYIIDFVDPGGPVDKAGIKIGDTIVSCNHYPLEEWFSDDHGQRAGDTLIFGM
                     LRNGIEVGTPVIVTSRLSMSPWIYWPPYIFFILVSGLSLVILHLKPKDRSATLYFLYI
                     QGSAIMAIGGFYLLEDPLSIFMALTFLFCGMLTPAILIHFHLIFPKTSVLFNRFKYLP
                     KIFYGAAFLFFLFHTVCIFYQSFITSFYAPLPFDPILIGLRWAAFASILAIAIAIFQL
                     FTIKDTLSRNQLLIIVTGTIFGVIFTIFYAVFYDYINSLWGVYPNLVQFSMRISSIIL
                     VICLLIAIFRFRIWQMEIVLKKVFLYIIATAIIILSYLGLLYLVDFFTLEETKTTRFI
                     ALALSVLIFLILRDRIQRLIERMFHRENYDSTTVVSEFEEGLGGVYRIEDLGYTILVR
                     MDHIFHFNSAALYLNKEKQTYESGFAIGTTGQQPRIEFKISKAFENKLLKQRVFSPAE
                     LSEATVLPGLEQVDLVVPMIKDGTPFGFFLCGPKKSEKAYSMQDIRVLSLVAKRVIAL
                     FQTASLYQKDLDRQLMLERERARIAQDMHDDIGAGLTKIAMISERAMGEEEIGNREQA
                     TGNSDKEKGNLEPGTLEPGTNDRERMQKVATTAREMINQLNVIVWALNPRNDNLDSLI
                     SYTRRYFGEYLENMGLDFYMESPADVPEIPVTPDFRRNVIYGMQEAIHNAVKHGASTE
                     IRLEVKLEHHGMKISITDNGKGFDQAKPGSGGNGLLNMQKRAEELGGTFEITSEVGKG
                     TSVRFKVVV"
     gene            complement(9048..10274)
                     /locus_tag="PKZ74_03115"
     CDS             complement(9048..10274)
                     /locus_tag="PKZ74_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91774.1"
                     /translation="MAVLEREMLTALTDQIDEKEFAILTGPRQSGKTTLLNQLAERLK
                     QREEEVYSITLEDQSILSRLNQHPENLFDFVIRSAEKRQFVLIDEVQYLDNPSNFLKL
                     LYDKYQPKLKIIATGSSAFYIDRKFKDSLAGRKKLFELFTLDFDEFLTFRTGASDLNE
                     ELNRIRMYKNYISSRRKELNSFFIEYLTYGGYPAVVLAASPERKIEKLKELMNSYLKR
                     DISESNIQEQDRFYRLIMILAQQTGSLVNVNELSKTLKLSVTAVENYLYILQKCFHIQ
                     LVRPYYSNIRKELIKMPKVYFHDSGLRNILLGQFNPVEQRIDKGMLIENYAYIRLRQL
                     YGNDTIRYWRTADGHEIDFVINPASGNGSAIEIKFDEENFNPKKYHNFLVAYPDYRIT
                     CRAFHASGNTSSLMAL"
     gene            10561..12318
                     /locus_tag="PKZ74_03120"
     CDS             10561..12318
                     /locus_tag="PKZ74_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91775.1"
                     /translation="MKKFAILFFVFHFSFFTFHFASAQWGSSCLPEGITNQEQTQVDS
                     FHYYYPGCTEIEGGVYINGGVYNLNGLLGLETIGGDLSISCVQFTDMAGLDSLKTIGG
                     SLTFYDTQLTDMSGLGALETVGGDFTIGCFTNTGLYCNNPYLENLSGLQDLQSIGGTL
                     TVQYCESLTNIDDLAGLTQVSRIYIKANPILNSVEGLANIYPDSLVSLTIAGNPSLNS
                     CEVESLCSFLDDPAGQINIYDNGTGCDNPVEIATACGVELSCLPYGNYWLLSQADINS
                     FPQDFPDCNEINGTIYVIGGDIVNLEPLNQVNSVHGALILGHEWYGGGNPVLENLQGL
                     ENIDSITGTLHIQYCPMLDDLSELQNLSFVGGTLQLVSNDGLTRLSGLEGLTSIGSGI
                     DISANDRLKDLSGLENVTDVGSGILIRLNDSLASLAGIDNISAGSISLLNIYFNDMLS
                     ECDVASICEYLAAPNGEIDISGNAPGCNSPEEVLEECAVGVEESSVVGRQSSVVSYPN
                     PTDGLTQFQISIFDFEHVSLTIYNAQGQEVVVVLDEKMAAGEHTVRWDAGELPAGIYF
                     YQVRAKSVGQVSAGKIVKY"
     gene            12482..12946
                     /locus_tag="PKZ74_03125"
     CDS             12482..12946
                     /locus_tag="PKZ74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM91776.1"
                     /translation="MSEQPQKIKVAIVEDDKEMREGLESIVKFHPGLRLLSSYPSAEE
                     ALAKISDEIPDIVLMDIHLPGISGIECVRQLKPVVPHTQFMMCTVYEDYENIFDSLCA
                     GATGYLLKNSPPGQIADKLFISFETVRTHIHNIFVKLHVQSRTEAVNKVFGR"
     gene            complement(12997..14244)
                     /locus_tag="PKZ74_03130"
     CDS             complement(12997..14244)
                     /locus_tag="PKZ74_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91777.1"
                     /translation="MRDLLKTVIADQLTLTWSESFTSRNVPETLLKGEDIVVISGIRR
                     CGKSTLLHQIRSGYSEKDYYMNFDDERLLNFKVENFQLLHETFIELFGQQKTFWFDEI
                     QNVKGWERFVRRLHDYGNKVFITGSNATMLSRDLGTHLTGRFLKHELFPFSFREFLDF
                     NNIKTGIDNIYSTGEKAGIKRLFNEYFKTGGFPYYLKYEDDNYLKSLYESILYRDIMA
                     KHNLTSERELKELIYLLASNVSRPYSNNRLAASIGLKNATTVKNYLDYIQDTYLLFAV
                     NKFDYSARKQLHNARKIYFIDNALIRKLGFMFSEDKGRMLENLVFIELQRRGEEVFYY
                     KGKGECDFVLRDGNSITGAVQVTYSMTANETRDREIKGLLEAMDSYNLSEGIILTDDT
                     EENLSVNGNRILIMPVYKWLLRI"
     gene            14410..14889
                     /locus_tag="PKZ74_03135"
     CDS             14410..14889
                     /locus_tag="PKZ74_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91778.1"
                     /translation="MIDIYDSYTDTWSVDHMTNPLVMHACAGAEDHFLVAGGLVYEDP
                     DSLYLTSKVEIFTDPDCSVGVGPEQGDGGLQPAVSVYPNPTHGIVELQFTVYNLQSAT
                     LKIYNAHGQEVASLLDGMCYGDQVIRWDMSRLPAGIYYYRTSNIEHRQSTMGKIVKY"
     gene            complement(14911..16038)
                     /locus_tag="PKZ74_03140"
     CDS             complement(14911..16038)
                     /locus_tag="PKZ74_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPM91779.1"
                     /translation="MDTPFIFGKLATGENFTDREAEQQRLIQNFRSGTNTILISPRRW
                     GKSSLVHKSAQEAKKAEHAPIMVFIDLFNIRSEEDFYKCLAESVIRATSGRMEEIMSN
                     IKEFMKEWVPRISFSPDAHHEFSLSLDWAEIKKQPDEILNLAENIAVAKNRKIVICLD
                     EFQNIAFYENPLAFQKKLRSHWQKHQMASYCIYGSKRHMLLDVFTSPSMPFYRFGDMM
                     FLPKISIDYWIPFIQERFKSTGKDIDREQAERIATLTECHPYYVQQLAQQTWLRTEKA
                     VTIGIIDEALDNLVLQMSLLFQNITEDLTTSYVNFLKMILDGHTRYTSMENIDRYQLG
                     TSANVIRIRKALISKEIIDEYEGKAILLDPLYAIWLKKYYF"
     gene            complement(16412..>16661)
                     /locus_tag="PKZ74_03145"
     CDS             complement(16412..>16661)
                     /locus_tag="PKZ74_03145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM91780.1"
                     /translation="SGNAMNDNLGIGIICPLTSKIKHYAGCLVFKKDDINMLDDDSEI
                     ITFQVRTIAKERLISKTGEITKSQLEAIKKGLQEILTY"
BASE COUNT         4518 a         3626 c         3794 g         4723 t
ORIGIN      
        1 taaccgtcga aactatttct cggtgcaata cctgaacagc tcaaggaagt atcatattta
       61 caattttgaa ggggaaaagc tgccgaacat ctcattatat gaccagtacg ccaacctgct
      121 ttttcattcc agtaccaatt ccaatcatac gtgggaagca ggccccagca tcgaggcata
      181 ccattcttac cgcccacccc tgactgcagg cggatcagat gccgaatatc atgcccagaa
      241 ggtccggttc gagtacaagt cggtcattgc acgggctttg atgctgaaca tgaaaaccgg
      301 catcagccgt atcaatcata tagaaacgga tacgctgact taccagcgtt acgatttcca
      361 tctgatggga ggttacaatt tcggccgggg ttacagcatt tccttcagtt atgactacgg
      421 accgatggtg aacaacgggc tataccagtt tgccggtgat attaaaaacc acagtttcaa
      481 tatcagcccc agcatgatga aatcgtattt caaagagagg ataaacctga ccatattctc
      541 caatttcatc tacaggatgg atctgggtta catttccgta aacgtaaatc ccaaaatcga
      601 ggcatattta ttcaacgact ggtatgcggt ggccagtggc acctatcatt ttacccagca
      661 ggaatatacc gaccagacta cgcggaattc ttatgtttat gccgaagtat cgatccggaa
      721 gaggttcgga aaatcggact tcaacaaatg gcagaagcac acccgcaggc tgaaagtggt
      781 aatgttcaaa gacgataacg gaaacgggac caaggaagac aatgaacagg gcattccgtt
      841 cgtcaagacc aggctgaagc tcaccaatac cgacaatccg tatgtcagca ctcaattccc
      901 ggtggatatc attcttcttt cgaataaagc gggagcggtg aattacaaca aattacccag
      961 aggattttac gaactgaccg tcactccgct gaccgatatg aaggaatatt tttacgtgaa
     1021 ccggagtgct gaaaaactgg aactgatcaa aaatgccact tattatgtcc cgttccagaa
     1081 ggccagcaag atcagcggca aggtcacggt tcagcgccag aaattcatca aaacagggga
     1141 agagtcgctg gacctgacaa atatcaaagt gaccgcctat aataagcagg ggaacagcta
     1201 ctcttcattc acgctgtcgg acggcagctt tacgatctat gtccctgaca acaacagtta
     1261 ttatctcagg atggggaatg ttttcggcag caatttccgg attacgcaaa acgatatcaa
     1321 cgtggatgtt gccgggggaa aagtacctga agtggtattc aacgtagtag agaacagccg
     1381 ccaggtcaag ttcaaggggg ccaagccggc ggttaccgat actgcccagg cggagccgct
     1441 taagatcaag gtgttgcatg gcaagttcta tgaaaactcc aaagaagcag cggttaacaa
     1501 gaatgccgtt ccggtattca atatcccgga agccgcggtc caggaagaac cgataatccc
     1561 gggaaatttc tatgtcgtaa caggatcgga ttccacccgg acgcaagctg ttaagatcaa
     1621 gcggattatg gatgagaacg gcctggatgc acgcctcgga tataatgaat ccgaaggcat
     1681 gtattacatc ttcaccggat attatcccaa taaaggggat gcgaaggatg aactggaaag
     1741 gttaaaggcg gcgggcatgg ctgatgtgga gatcattaaa ttccaggaat aatcctattg
     1801 cctttcttaa tttttattaa aaatcacaac ttttcaccgg aaatagaatc cataccgcat
     1861 tttttgcatt tctgcaaatt tgaaagttaa ttataagata tagttcgttt cttcccagac
     1921 aaatgccata aggatcaatt tccctgatcg gaccggctga tttaatattg tgattgaatt
     1981 tcaacatatt aaatcgaagg ccatgagaac caggaaattt aattcaatgg taaaccgtcg
     2041 gcaggccagc agtttcatct cagccctgag gttatcgatt acatgtaccg gtaaaatatt
     2101 gaataaaaag tgactttcgg gtccaacttg tttcctttta attatgacaa gcaaacaaat
     2161 cagaaaatga atccggctca aaacatagtg attgcactgt tcctgacgat ccagctgggg
     2221 atcggcgaga accggattta tatcaaaccc tctgttttta cattgcctga gattatcttc
     2281 actaaccagg ccaattacga gctttcaaag aagaataaga ttcccaggac agatcctccg
     2341 gctatcagga tttatcacaa aaataatgtt catcatccgg aaaaatcgtt ccaggttaag
     2401 gttacaatgc ctgatctggt ggctgttttt catcgcggaa atggcctgga taactataat
     2461 aagaaaataa aatgaaaacc agacagacag cattgataac gggagccacg agcgggatcg
     2521 ggaaggcttt tgctgtctgt tatgccggta tgggatacga cctggtggtc accgggagga
     2581 ggatggacag gctggaacag ctttgttcgg aattatcccg ggaatacggg atcagggctg
     2641 aagcggtcaa agccgagctg tcggaagaac aagacatgga gaggctgctg aaagtgatta
     2701 tacgacggga caatatcgga attctggtca acaatgccgg ttttggctcc ggggttgatt
     2761 tttaccaaaa cagcctggaa gagcacctga aaatgctgga tgtgcacgta aaggcatcga
     2821 taaagctcat atatgcagta ttgcctcaga tgaggatgag aaaggagggg gtgatcatca
     2881 atgtctcgtc gctgggggct ttcatgccgg cgccgggaag ttcgatgtat tctgccacca
     2941 agctgttcct ggccagtttc tctgaatcgc tgaatatgga ggtcaggcgg catggcataa
     3001 aggtccggtg tgtttgtccc gggtttaccc ataccgagtt ccatgaacgc cgggctcagg
     3061 gcaagatcag ggcgggcggc aagctgatgt ggatggaggc ggatgacgtt gtgagaaaaa
     3121 caatcagatc gctgaagaag aataagatag tggtagtgcc gggaatcatc aacaggttat
     3181 tgattgctgt gacgagcatc atcccacgcc ggttgtatta tcatttgatg gaaaggtcag
     3241 gaaaggccaa ggtcggtccg ggctttatcg atcgtatgaa gcaacttgtt gaaaaaccgg
     3301 tttcgttctt tttcaccact cacactgatc aggtttgatt tcttcatcag ctgccgcata
     3361 tgctgctcga cattggtaca gaaggcctta tcctgtgtga cgaggaggct gatcacgtcg
     3421 aaggtcaggt aagttgccat atggtcgccg acacgcaccc agatcgaatt atcgttgagc
     3481 acgacttcat tttcgtacat catgaatgaa ttcggaatcc cctccgccgg catcccgtaa
     3541 tggaatttaa accccatttc agcctgcttc cgaatatgga ggacccattt ttccagttta
     3601 tccaccaggt ttaagatatc ctctttcttc tcaaaatatc ctgatatcca gtaatattcg
     3661 atctgccgca tcaggattcg gaatgtatcc tcgttccaga tctcggtggt tggaatttta
     3721 atagctgccc tgagaatatc atttccgata tctactatct cggggttgac atcatccagg
     3781 cgaaacaatt tattttcata ttcggggaag ttgaaaatcg ttttctccca gaagaagaat
     3841 ttaaaagcag cgatctcggg gaaatggaaa taatggaaca ccggaggatc tttggcggca
     3901 tagataattt cagggttcct ggctgtctga attctccgga tatcatccct tatctgcagg
     3961 agccagtctt tgatattaaa gtcgcccgga tgcaaagcaa atttccggaa gacgacgtgg
     4021 ttaggatcga ggccggcgag agcatccagc gagagattga attttttact gagtttaacg
     4081 agttcttcga gcgacagttc cttctcccct cttatccttc ggtaagcact gtcgtaactg
     4141 atttccagca gttcggcaat ttcatggaca aaagacatgt tatcggggat cttttccctg
     4201 atctgttgaa aaaagaattg ctgaagacct ggcgtttcag tcataatgat gtaaatataa
     4261 gtaatttata tttacattga atttttaccc ggaaaaatgt tttcgtagcc cgtcgggctt
     4321 cagatttcgt gctccgaggg gtaaacccgt cggttatggg gatatgaagt ttattcgatg
     4381 gagattttgc cggcgttttg atcgagccac tgatcgaaac tttgcagtga cgggttgata
     4441 gacctggcta cttcaaggtt gctgccgccg acgaaatcgg cttcaaaatc gcgtttgaac
     4501 tggaacatgt tacccatttc atcggcgccg gggaagccga aactgcggta gacatctgcc
     4561 ggcacatcgt tatatttcac ttctgcacct atgaccctgg tgaaagaggc tgccatctgg
     4621 gcgccggtga ggtgctcccc tgcgattccg atggttttgt ttttgtattt ttcgcctgcc
     4681 ttaaaaatcc cataagcaca cttaccgata tcgacggcga ctatgcccgg aagtttttta
     4741 tcgcccatcg gaaaagtgat cgccagtact ccgtccggtc ctttctttgg gccaaggccg
     4801 aaataaatgt aattatccca gtagaaggaa gttctgagga tagtcgacgt taaacccggt
     4861 tgttgaaaga acttttccgc ctctcctttt gagtcgaaat gaggcacttt gtattttccc
     4921 atcagggtcg gcatccggtt gtcgtcaagc gggataaagt cacgggtatc ttccagcgac
     4981 gaccagataa catgctttat gcctgctgcc agagccgcat cggcaatatt ctttgcctgt
     5041 gctgtttctt tttcgggtga aaaatgttcc cagaaattgg taacgccaaa tacaccatat
     5101 gctccgttaa aggccttttc caggctttca gtatcgtcga gatcagccga tactacttcc
     5161 gcgcccctgt cggccagggc tttagcttta tcagcattaa tattacgggt aatagccctt
     5221 accgaaaatt cgctttgtga atcggataaa atggcatcgg ccagtccgcc accctgagcg
     5281 ccggttgcac cgattattgc tattattctt ttttcaatca ttatttagtt aattaaagtt
     5341 gacaatcata aactcataga aatgcatttt gttcatttgg ctgacgattg aaatgggaaa
     5401 atcgtgaagt actggcaaac cggcattggt tcctggttcg gggtttttta aattaatatg
     5461 tctgatttgt ttacaattta agtatatctg tccttgtatt ttaaaataac gtcttttaat
     5521 tttcaaggat aaattatgat tcttcgtcca cgactcaagg ccgaaattga acgatcctta
     5581 tcggtttcac cggtggttgc gcttctcggt ccgagacaat gcggcaaaac cacgatcagc
     5641 aggcttattg caaagaatca taaggcagtc atatttgacc tggaagatcc tgttgattac
     5701 cagcaattat ctgccgcacc ccttatcaca cttcaatcgc taaatgggct tgtcattatt
     5761 gataaagtgc agaagatgcc tgaattaact ggcatcttaa gggttttagc tgaccgcagc
     5821 aactctcctg caaagtttct gatactcggc agcgcttcac ctgttatgat gaagaatgct
     5881 tctgaatcgc ttgccggcag ggtgtctttt attgacatgt cgggctttaa cctggaagaa
     5941 atcggggcgg aacacctccg gatgctttgg accaggggtg gatttccacg atcattcctg
     6001 ggcaaagatg aacggataag cttccaatgg agacaggact ttatccgtac ttttctggag
     6061 agagatattc cccagttggg aatttctatc ccggcggttt cattgagaag attctggaca
     6121 atgctcgccc attatcatgg aaatatctgg aacggttctg aatttgcacg ctccataggg
     6181 actaccgaac caacagccag aagatacctt gatattcttt ccggcgcttt tgtagttaat
     6241 caattacagc catggcatga aaatctaaaa aaaaggcagg taaaatcacc caaagtttac
     6301 atcagggact cagttttact tctgggccac tcacgccggg ggagaaattg atctgctttc
     6361 aatacgcaac ggcattaaaa caggatttga gatcaattac tctgatgcac cgaaggtgac
     6421 caaatcaatg ctccttgcaa tcgaagatct ttccccggaa aggatattga ttatttaccc
     6481 gggtgagaaa gattatgcgc tgcatgataa aattcaggtg aaggctgcga agaatatctg
     6541 gatttcttcc ctacctttac ccgtgtaaac caacctctct gccttgaacg ccatcagacg
     6601 attcataaaa accgttccga aatgggcatt tgtggttgtg ctggtcgtga ttttcgtgca
     6661 aacagccaat atgttctaca atgcccgcat gccggtcagc ggtgaaagag aaaacaacga
     6721 caacaccaat ggttattata tcattgactt tgtcgatccc ggcgggccgg tagataaagc
     6781 agggatcaaa atcggcgata cgattgtttc ctgcaatcat tacccgctgg aggaatggtt
     6841 ttctgatgat cacggacaga gagccggcga cacccttatt tttggaatgc tgaggaacgg
     6901 catagaagtg gggacgcctg ttatcgtcac atccaggctg tcgatgagtc cctggatata
     6961 ttggcctcca tatatatttt tcattctggt cagtggatta agcctggtca ttctacatct
     7021 gaaaccgaag gaccggtcag ctactttgta tttcctgtat atccagggat ctgccataat
     7081 ggccatcggg ggtttttatt tgctggagga ccctttatcc atatttatgg cactgacttt
     7141 cctgttttgt ggcatgttaa caccggctat ccttatccat ttccacctga ttttcccgaa
     7201 aacatctgtt cttttcaatc gctttaaata tttaccgaag atattttatg gagccgcctt
     7261 tttattcttt cttttccaca cagtctgcat tttctaccag agctttatca cttcatttta
     7321 tgctcctctt ccgtttgatc ccatattaat aggccttcgc tgggcagctt tcgcttcgat
     7381 tttagccatc gccattgcca tattccagct attcaccatc aaagataccc tgtcccgtaa
     7441 ccagttattg ataatagtga ccggtaccat ctttggcgtg atattcacca ttttctatgc
     7501 ggtattttat gattatataa acagtctttg gggtgtatat ccaaaccttg tacaattcag
     7561 catgaggatt tcaagcatta tcctggtcat ttgcctcctt attgccattt tccgtttccg
     7621 catctggcaa atggaaatcg tcctgaaaaa ggtatttctt tatatcattg cgacggcaat
     7681 tatcatttta tcctacctgg gcctacttta cctggtcgat ttcttcaccc tggaagaaac
     7741 aaaaacaaca cggtttatag cgcttgcctt atctgttctc attttcctga tcctgagaga
     7801 ccgtattcaa aggcttattg aacggatgtt tcacagggaa aactatgatt cgacgacagt
     7861 tgtatcggaa ttcgaggaag ggcttggggg agtatatcgg attgaggatc tgggatatac
     7921 aatcctggtt cggatggatc atattttcca ttttaattcg gccgctttat acctgaataa
     7981 agaaaagcag acttatgaat caggttttgc gattggaact accggccagc agccccgcat
     8041 cgaatttaag atcagcaaag cgtttgagaa taagttattg aagcaaaggg tgttctcccc
     8101 tgctgagtta tcagaggcca ctgtcctgcc aggtcttgaa caggtggacc tggtagtccc
     8161 gatgatcaaa gatggcacac cgttcgggtt tttcctttgc ggcccgaaga agtcggaaaa
     8221 agcttattcc atgcaggata tccgggtgct ttcgctggtg gcaaagaggg tcattgcgct
     8281 gtttcaaacg gccagccttt accagaaaga cctcgaccgt cagctcatgc tggagcggga
     8341 acgtgcacgc atcgcgcagg acatgcacga cgatatcggc gcggggctga cgaagattgc
     8401 catgatcagt gagagagcca tgggggagga agaaataggg aacagggaac aggcaacagg
     8461 gaatagtgat aaggaaaaag gaaaccttga acctggaact ttggaacctg gaaccaacga
     8521 cagggagcga atgcagaagg tggctactac tgcccgggag atgatcaacc agctgaatgt
     8581 gatcgtgtgg gccttgaatc cccgcaacga caacctcgac agccttatat catacaccag
     8641 gcgctatttt ggagaatacc ttgagaatat gggtttggat ttctatatgg aatccccggc
     8701 tgatgtacct gagattccgg tcacccctga tttccgacgg aatgtcattt acggtatgca
     8761 ggaagcgatc cacaacgccg tcaaacatgg cgcctccact gagatccggc tggaagtaaa
     8821 gctggaacac cacggcatga aaatctccat tactgataac ggcaagggat tcgaccaggc
     8881 taagcccgga tcgggcggga acggactgct gaacatgcag aagcgggcgg aagagctggg
     8941 cgggacgttt gagattacca gtgaggttgg caaggggacg agcgtgcggt ttaaggtggt
     9001 tgtgtagcag ttggacagtt ggaacactgg aacattggaa caattgatca caaagccatc
     9061 aatgaactgg tattccctga agcatgaaat gcccggcaag tgatccgata atccggatat
     9121 gccacgagga aattgtggta cttcttagga ttgaaattct cctcatcaaa tttgatttct
     9181 atggctgatc cattaccgga ggccggattt atgacaaaat caatttcatg tccatcggca
     9241 gttcgccaat atcggatcgt gtcattcccg tataattgcc ttaaacggat gtacgcataa
     9301 ttttcaataa gcattccttt atcaatgcgt tgttcaaccg gattgaactg accaagcagg
     9361 atattgcgca gcccggagtc atgaaaatac accttaggca tcttgatcaa ttcttttctg
     9421 atattactat aatagggacg tactaattga atatgaaaac atttttgaag gatatacagg
     9481 taattttcga cagcagttac agataattta agtgtttttg aaagttcatt gacattgaca
     9541 agtgaccctg tttgctgtgc cagtatcatt atcagacggt aaaatctatc ctgttcctgg
     9601 atatttgact ccgaaatatc ccttttcagg tacgaattca ttaattcctt cagtttctca
     9661 atctttcttt ccggtgaagc tgccaggacc actgccggat atccgccata cgtaagatat
     9721 tcgatgaaaa atgaattcag ttctttacgc cttgacgaaa tatagttctt atacatccgg
     9781 atcctgttca attcttcgtt taaatcgctg gcgccagtcc ggaatgtcag gaattcatca
     9841 aaatcaagcg taaaaagctc aaaaagcttc tttcttccag ccagggagtc tttgaatttc
     9901 cggtctatat aaaaagcact gcttccagtg gcaatgatct ttagctttgg ttgatattta
     9961 tcataaagca gtttaaggaa atttgaagga ttatcaagat actgaacctc atcaatcagg
    10021 acaaactgcc ttttttcggc agatcttatt acaaaatcaa atagattttc aggatgttga
    10081 ttaagacggg aaaggatcga ctggtcttcc agcgtaattg aatatacttc ttcttccctt
    10141 tgttttaatc tttctgccag ctggttaagc agggtggttt ttccgctttg cctcgggcca
    10201 gttaatatcg caaactcctt ttcatcaatt tgatcagtaa gtgctgttaa catttccctt
    10261 tccagtacag ccataattat aactttaggt caaagataat ataattttta taaatgattt
    10321 ttatccgatt attttcattt attaagtaac attcttcttt cgtcaaaaac tgctgatatt
    10381 tccgatccaa aaaatccccc gaaatacgta ttgacttcgc cggcggggag gagtaaattt
    10441 gtgagtcgtt ggccgttggt cgttagtcat taatacatta ctaattaacg cattacgcat
    10501 taagacgtta ctcattcctc attaaacatt aagcattaag cattaataaa gtatattgcc
    10561 atgaaaaaat ttgccattct atttttcgtt tttcattttt cgtttttcac ttttcatttt
    10621 gcctcggcac agtggggcag ctcctgcctg ccggaaggga tcacaaatca agaacagacg
    10681 caggttgata gcttccatta ctattatccc ggctgtaccg aaattgaagg gggtgtttat
    10741 ataaatggag gcgtctacaa tctgaacggt ttgctgggat tggaaactat cggcggggac
    10801 ctgtctatta gctgcgtcca atttacggat atggccggcc ttgattccct gaagacgatc
    10861 ggtggcagtc tgactttcta tgacacgcag ctcacggata tgagcggtct gggcgccctg
    10921 gaaacagttg gcggagactt cacgatcgga tgttttacta ataccggatt gtattgcaat
    10981 aatccatacc tggaaaacct ttcgggtctc caggatttac aaagtatcgg aggcacattg
    11041 acggtacaat attgcgaatc ccttaccaat attgacgatc ttgccggatt aacccaggta
    11101 agcaggatct atatcaaggc aaatccaatc cttaattctg ttgaaggcct tgccaatata
    11161 tacccggatt ccctggtttc gctgaccata gcaggaaatc cttccctgaa ttcgtgcgag
    11221 gttgaaagcc tgtgcagctt tctggatgat ccggcagggc aaataaatat ttacgacaat
    11281 ggaacaggct gtgataatcc cgtcgagata gcgacagcgt gtggcgtgga gctctcatgc
    11341 ctgccatacg gcaattactg gctcctgtcg caggcggata ttaacagttt tccgcaggat
    11401 ttcccggatt gtaatgagat aaatggaacc atttatgtta tcggtgggga catagtcaac
    11461 ctcgaaccat taaaccaggt taactcagtt catggggccc tgatattggg tcatgaatgg
    11521 tatggcggag gtaatccggt cctggaaaac ctgcaaggtc ttgaaaatat tgattccata
    11581 acaggtacgt tgcacattca gtactgtccg atgctcgatg atctctcgga actccagaat
    11641 ttatcatttg tcggaggtac attgcagttg gtaagcaatg atggcttaac ccgcttatca
    11701 gggctggaag gactcacatc aatcggttcc ggcatcgata tcagtgcgaa tgatcgcctt
    11761 aaagatctat cagggcttga aaacgtgaca gatgtaggaa gcggtatttt aatccgcctc
    11821 aacgattcac tggccagcct tgcaggaata gataatattt cagccggttc catttcatta
    11881 ttaaatatct atttcaatga tatgctttcc gaatgtgatg tggcgagtat ttgtgaatac
    11941 ctggctgcac ctaacggaga aattgatatc agtggaaacg ccccaggctg caacagcccg
    12001 gaagaggtgc ttgaggagtg cgcggtagga gttgaagagt cgtcagtcgt cggtcgtcag
    12061 tcgtcagtcg tcagttatcc gaatccgacg gatggattga cccaatttca aatttcaatt
    12121 ttcgattttg agcacgtgag tttgacaatt tacaatgccc agggccagga agtggtggta
    12181 gtgcttgatg agaaaatggc ggcgggagag catacggtgc gatgggatgc aggtgagcta
    12241 ccagcaggga tctacttcta ccaggttagg gcgaagagcg tggggcaggt tagtgctggg
    12301 aagatcgtga aatattagtt ggacagttgg acagttggac ggttggacgg ttgacaagtt
    12361 gaaacactgg agcaccggaa cactggaact tttaaacgtt ggaaccctta aactctgaaa
    12421 ccccaaaata tcccccgaaa tacgtattgc attcagcttg ttttcaattt aactttgtga
    12481 aatgtccgag cagccacaga aaataaaagt cgcaattgtc gaggatgata aagagatgag
    12541 agaagggctg gagtcgatcg taaagttcca tcccgggctc cggctcctct cctcctaccc
    12601 ttccgctgaa gaagcactgg caaagatttc cgacgaaatt cccgatatcg tgctgatgga
    12661 tattcacctt cccggaatat cggggattga atgtgtaagg cagctcaagc cggtcgttcc
    12721 ccacacccag ttcatgatgt gcaccgttta cgaggattat gaaaacatat tcgattcgct
    12781 ctgcgccggc gccaccgggt acctgctgaa gaacagccct ccgggccaga ttgccgataa
    12841 gctgttcatc agttttgaaa cggtcaggac gcatattcac aacatttttg tgaaactcca
    12901 tgtgcagtcg cggacagaag cggtgaacaa ggtgttcggg agatagtgtt atgtattgag
    12961 gattaagggt taagtagtca gtgcgatgac ctcttttcag atcctaagca accatttata
    13021 aaccggcatg atcaatatcc ggtttccgtt gacactcagg ttttcttctg tatcatcagt
    13081 cagaataata ccttccgaaa ggttataact atccattgct tccagtaagc cctttatttc
    13141 cctgtccctg gtttcattag cggtcatgga ataggttacc tggacagcgc ctgtgatgct
    13201 atttccatca cggaggacaa aatcgcactc tcctttccct ttataataaa acacctcctc
    13261 gcctcttctt tgcagttcta taaacaccag gttttcaagc attcttccct tatcttctga
    13321 aaacataaat ccaagcttac gaatcagcgc attatcaatg aaatagatct tcctggcgtt
    13381 gtggagttgt ttcctggcag aataatcaaa tttattcaca gcaaataaca gatatgtatc
    13441 ctgaatataa tcgagatagt tttttaccgt tgtggcattt ttcagtccta ttgaagctgc
    13501 aagcctgtta tttgaatatg gccgggatac attgctggcc agtaaataaa tcagttcttt
    13561 cagttccctc tcactggtta gattatgttt tgccatgata tcccggtaga ggatactttc
    13621 gtacaatgat ttcaggtaat tatcatcttc atatttaaga tagtatggaa accctcctgt
    13681 tttaaaatat tcattaaaca accgtttgat tcccgctttc tcgccagtag aataaatatt
    13741 gtcgattccc gtcttaatat tgttgaaatc cagaaattcc ctgaaggaaa atggaaatag
    13801 ttcgtgtttc agaaatcgtc cggtcagatg agtaccaagg tccctgctga gcattgttgc
    13861 atttgaacct gtaatgaata ccttgtttcc ataatcgtga agccgtctta caaacctttc
    13921 ccaaccttta acattttgaa tctcatcaaa ccagaaagtt ttttgctgcc cgaacagttc
    13981 aataaaagtt tcatgaagaa gctggaaatt ttctactttg aagttcagta gcctctcgtc
    14041 gtcgaaattc atataataat ccttttccga atagcctgac ctgatctggt gtagcaaggt
    14101 agatttaccg caacgcctta tacccgaaat aaccactata tcttcccctt tcagcaatgt
    14161 ttccggtacg tttctactgg tgaatgactc actccaggtg agcgtaagct ggtcagcaat
    14221 gacagttttt aaaaggtctc tcatttggtt tgtattttat acaaatgtat tttatttcta
    14281 ttcataatac aaatatttct atttattttt ctattattat tagaagttag ttcattatca
    14341 tttgctaaaa tttggaattg aaagacaaaa atacgggagg cgcaaataag ctggaactcc
    14401 ggatctgatg tgatcgatat ctatgattcg tacacagata cctggtcagt tgaccatatg
    14461 acgaatcccc tggtcatgca tgcctgtgcc ggggcagagg atcactttct ggtggccggc
    14521 gggcttgttt atgaggatcc cgattcactt tacctgactt ccaaagtcga gattttcaca
    14581 gatcccgatt gttcggttgg ggtcggtccg gagcagggag atggcggctt acagcctgcg
    14641 gtcagcgttt atcccaaccc tacccatggg attgtcgaat tacagtttac ggtttacaat
    14701 ttacaatctg cgaccttgaa gatttataac gcccatggac aggaagtggc gtcgctgctg
    14761 gatgggatgt gttatggcga tcaggtgatc cggtgggata tgagcaggct gccggcagga
    14821 atctattact atcgaacatc gaacatcgaa catcgacaat cgacaatggg aaagattgtg
    14881 aaatactgaa ggcgatttct tcacatagcc ttagaaataa tacttcttta accagatggc
    14941 gtatagcgga tcgaggagaa tggctttccc ttcatattca tcgattatct ccttgctgat
    15001 cagcgctttc ctgatcctga taacgttggc ggatgtgccg agttgatacc tgtcaatatt
    15061 ttccatcgac gtataacggg tatgcccgtc aaggatcatc tttaaaaaat tcacatagga
    15121 agtggtcaga tcttccgtga tattctgaaa taataggctc atctgtaata caagattgtc
    15181 caatgcttca tcaatgatac cgattgtaac ggctttttcg gtcctgagcc aggtttgctg
    15241 tgccagttgc tgcacatagt agggatggca ttcggtcaat gttgcaatcc tttctgcctg
    15301 ctcacgatca atgtcttttc ccgtcgattt gaaccgttct tgtataaagg gtatccagta
    15361 atcgatggaa atcttcggca ggaacatcat gtctccgaat ctgtaaaaag gcatggatgg
    15421 agaagtaaat acatcgagca gcatatggcg tttgctcccg taaatacaat acgaagccat
    15481 ctgatgcttt tgccagtgcg accggagctt tttctgaaag gccagtggat tttcataaaa
    15541 ggcgatgttc tgaaactcat ccaggcagat gactattttg cgattttttg caacggcaat
    15601 attctcagcc agattgagga tctcatccgg ttgtttcttt atctcagccc aatcaaggct
    15661 aagggagaat tcatgatggg catccgggga aaaactgatc cttggaaccc actccttcat
    15721 aaactcctta atattactca tgatctcttc catccgtcct gatgtagccc ggatcacact
    15781 ttcagccagg catttgtaaa aatcctcctc actccggata ttaaaaagat caataaatac
    15841 catgataggc gcatgttccg ctttcttagc ctcctgggca gatttatgta caagcgaaga
    15901 tttgccccac ctgcgcggag aaatcagaat ggtattggtc cctgaccgga aattctgaat
    15961 aagcctttgt tgttcagcct cccggtctgt gaagttttca cctgtggcga gctttccaaa
    16021 tataaaagga gtgtccataa ttataatttt aatcaaagat aagtaattat tttgtaacta
    16081 atctttttgt tagttaatag ttgcgtactg atgttggaac actggaatcc caagaatccc
    16141 cggaaatacg tattgatttc gatggcgggg agaggtaatt ttgcaggtag tcggttgtca
    16201 gtcgtcagtt atccgagtcc atcccatggg aaagatcgcg aaatactaag aatatcgttt
    16261 tcttaaatgg ccaagatgat gaacctacgt aatattccgt gatcattcgt tgtattcgtt
    16321 tcattcgtgt tcactctatt gacagcacac gaatggcgcg aatgcaacgg atcttcacga
    16381 atgtgttaca accgaaccaa tacttccatc ttcaataagt cagaatctcc tgcagacctt
    16441 tctttatagc ttcaagttga gatttggtga tctctcctgt tttggagata agtcgctctt
    16501 tggctatcgt cctgacctgg aaagtgatga tctcggagtc gtcgtcaagc atgttaatat
    16561 catctttttt aaagaccagg caaccggcgt aatgctttat ttttgacgta agcggacaaa
    16621 taatgcctat tcccaggtta tcgttcatgg cattgccgct g
//
LOCUS       DAOPNH010000034        16550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3422_length_16550_cov_6.209942, whole genome shotgun sequence.
ACCESSION   DAOPNH010000034 DAOPNH010000000
VERSION     DAOPNH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_16550_cov_6.209942"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKZ74_03150"
     CDS             complement(<1..728)
                     /locus_tag="PKZ74_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPM91781.1"
                     /translation="MENLVIDTNLKYKVKDISLAEWGRKEIGMAEKEMPGLMSLREKY
                     GPQKPLAGARISGSLHMTIQTAVLIETLTELGADVRWASCNIFSTQDHAAAAIAQRGI
                     PVFAWKGETLDEYWWCTYQALSFPGGKGPHLVVDDGGDATLLLHWGVQAEKEPSLLNR
                     KVESQDETAIMDLLRKVYVEDNRKWQRTTSEWRGVSEETTTGVHRLYQMQERGELLVP
                     AINVNDSVTKSKFDNLYGCRESLA"
     gene            813..1454
                     /locus_tag="PKZ74_03155"
     CDS             813..1454
                     /locus_tag="PKZ74_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPM91782.1"
                     /translation="MAEYLRRQIGNHSLLALWLITESTEELEAAISLRAEEKKLYESF
                     VAESRRKQWLAYRLLIRDLLRPDDFPVEYDETNKPYLAGSNYHISVSHSGDMAAVIIS
                     RKSRVGIDIEKIRPRVGKVKERFLSLPELDRIGNDSGFSRLTLAWSAKEAIYKLFGQR
                     DLDFRENICLHLPDQPGEPFDGDIILDSGITRFRIENELIGDYILVYVEEPAP"
     gene            1432..2250
                     /locus_tag="PKZ74_03160"
     CDS             1432..2250
                     /locus_tag="PKZ74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPM91783.1"
                     /translation="MSKSRRRDRFPAGSINFYTFASEQQEMTIYQKITGKIPGKAFAV
                     LIDPDQYHTGGIDNIVKIASDSRVDFILVGGSLLTNDHLDETLERIRSLAEIPVILFP
                     GSIMQVSGKADAVLLLSLISGRNADLLIGKHVIAAPYLKESKLEILPTGYMLIESGPL
                     TTAQYISNTLPIPHAKDDIAACTAMAGEMLGLRLIYMDAGSGAENPVSPSMIRKVRAV
                     ISVPLIVGGGIRTPEQAIVSCQAGADIIVVGNAIEKDPSCLKEIAHAIHSCSEL"
     gene            2306..4438
                     /locus_tag="PKZ74_03165"
     CDS             2306..4438
                     /locus_tag="PKZ74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPM91784.1"
                     /translation="MKKNISLILVFLFATSLAFAQKKLTIEDVSGMNRSVYPSSMRNL
                     QWVPESNRFSYMENNALLTRKADKTVPDTLVRLSEMNPALKKASFDTLARFPFISWED
                     ESSFLFTNNQKVYRYFVNSRSVSFVNSYPEEAENIDATGNMKKIAYTVDNNLYLALEG
                     KQVQLTQDTLSGIVNGQSVHRNEFGIEKGTFWSPEGNYLAYYRKDETMVTDYPLVNTT
                     TRIAELENIKYPMAGMTSEQVTLVVYNLADGTRLTVKTGLPADQYLTSVTWSPDEQDL
                     YIGILNRGQNHLKLNRYSATTGEYEATLFEEKSDKYVEPLHPLYFIDSQPDRFIWMSQ
                     RDGYRHMYLYSTDGTLLRQLTSGPWIVTSFLGTDPKGNRAFFLATRNSPLNEDIFAVD
                     FKTASIQPVSANTGVHNPALSPDARYILDVYSDTSVCREYNIIDSKGKMLQVISRSDD
                     PLDDYVKCQSKIFQLPADDNTPLFCQMILPHDFNPAKKYPVIVYVYGGPHAQLVNNTW
                     LGGTSLFNYYLAQQGYIIFTLDNRGSANRGRDFEQAVFRNLGTLEVIDQMTGIRYLKS
                     LPYVDPERIGVTGWSYGGFMTISLMLRQPGVFKVGVCGGPVTDWKYYEVMYGERYMDT
                     PEENPDGYREASLLERAGELKGDLLIIHGTSDPTVVWQHSLALIQRFIEEGKLVDYFV
                     YPGHGHGVGGKDRLHLNRKIEQYFIDHL"
     gene            4435..6696
                     /locus_tag="PKZ74_03170"
     CDS             4435..6696
                     /locus_tag="PKZ74_03170"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPM91785.1"
                     /translation="MIRWIILPVILFFSVSGYFSATAQILLADSGLSDYRIVIPSKPS
                     AGESKAALQFQSVFREISSVTLPIVPDSRPDSEKEIVIGNSTRLEKIKSKSRFKDDED
                     DGFLIHTDQAKLFICGAGDNGLFYGVCSFFENYLGYRYYSPSVKHHPILNKVIINKID
                     DRQVPVFTFREMLFPARSDSAYRQWHKLDTHSDGSWGMWVHTFDDLVPAAKYFDEHPE
                     YFSEINGNRVRDGQLCLSNPQVFDLVIENLRKKIIARPEALYWSVSQNDNFLACQCAS
                     CDSVAKKFGGQSGLMIWFVNRVAELFPDKIISTLAYQYTRHAPENIRPLPNVNIMLCS
                     IECNRSKPLRNDPSSADFVKDVKDWTRLTENNLVWDYVVQFRNYISPFPNLRVLQPNL
                     EFFAGKGCKLMFQQGSGSSLSEFSDLRAYMIAKLLWDPYADADAIMKDFIWGYYGDAA
                     PHILDYIGILHDELESSGGDLVIYGFPYTGVHSFLKPFLIPAYRKCFDEAENAVKDNP
                     GLLARVHYARLPLDFAILDLSLHQVDHDLTWFTGDGMNLTVRQDMINLLDTFVYRCKR
                     LDVNTLNEQSYSPEEYRLTVKKYLLKTSERHMGIGKAADILTEYSAKYEAGGENALTD
                     GIRGINDYHFNWLGFEGNDLEAIIDLEEEKTLASISADFLQDIRSWIFLPNSFSAYYS
                     LDGIIFHEIGTDPNITPDNKTGAFINTFTVPADGIRARYVKVVAESMKTCPEWHLGKG
                     KNSWIFIDEIVIK"
     gene            6798..9275
                     /locus_tag="PKZ74_03175"
     CDS             6798..9275
                     /locus_tag="PKZ74_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HPM91786.1"
                     /translation="MSFNIRFDNPADENNCWENRRKLATLTIRNVAPDFIGMQEALER
                     QVLYLDASLPGYDYIGVGRDDGKKAGEYVPVFYRTDRFTLLDQGHFWLSETPGDTGSV
                     GWDAALPRICTWGKFRETETGREILFLNTHFDHMGAKARAESARLILGFIGENMHTLP
                     VILTGDFNCSADEEPYRILTQGTGLSDACLASGDTNKCSEGTFNGFGHEQNPQRIDFI
                     FFNEQFRTRSYEARKITYNGMFVSDHWPVLAQLECSEKGARPDERSEEGPAGRGQGDT
                     TIVQTFTFDSIVTRRAEFQFPGKEKRYEKILMYYTLKCDSLTPHDQYPCGEWDYTTYV
                     KVFVPTGRYDSLFYTRTDSLGNVVRSWFGESIEVREPFEIGRFITPYGKRLDLGLNGF
                     TWIYDVTDYEPLLHGTVDLKAGNGQELLDLRFLFIEGIPPRDVVSVRNIWPEGSYTYK
                     ELADDAKLQTVRQKLADNASTFSIRARISGHGHFGPFNCCEWDPKTHTYLVNGENRFD
                     WKVWRDCGMNPVHPQGGTWQFDRAGWCPGTFVDTYDFEITPFVEPGEEVSIDYLIQPY
                     DTANGEEGGNFEMAMHLIEYSSPNYKLDLELTDILAPGNRHEYRRMNPVSLNPVIRIR
                     SLGTDTVRNITFFYGLEKGEKITYDWQGKLGFMQSADIILPKPSWQGADDKSMFIAEI
                     ITVNGLQDERESNNTQVSAIVKPVILPDKFIIHVKTQGFGRAADNAWKIKDETGRVVY
                     ERLDFLDDTVYDDPVSLPPGAYDFTFTDKNEDGLIRHWWLHWEDPGKVGQNGELKILG
                     SDEKEIMNLGYDFAEKRTLQFFVGEPE"
     gene            complement(9296..9631)
                     /locus_tag="PKZ74_03180"
     CDS             complement(9296..9631)
                     /locus_tag="PKZ74_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPM91787.1"
                     /translation="MDSTRQHKMARLLQKEMGDIFEIDMRRLFPGAMITITKVNVTKD
                     LSVARFNLSIFALKDKKPTVEEIRAHIPEFRYKLGNKIRKQVRIIPQVEFYEDDSLDY
                     IENLEKLMK"
     assembly_gap    9645..9744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9745..11156)
                     /locus_tag="PKZ74_03185"
     CDS             complement(<9745..11156)
                     /locus_tag="PKZ74_03185"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPM91788.1"
                     /translation="MSSLEEKYRIFEEKDKQAELGGGLERIDKQHKAGRLTARERVEL
                     LLDPGTFVEKDKFMTHRAKDFDMDKNKIPGDGVVTGYGKIDDRLVYVFAQDFTVFGGT
                     LSRANADKIVKIMDLAMRMGAPVIGLNDSGGARIQEGVESLAGYADIFFRNVMASGVI
                     PQISAVLGPCAGGAVYSPAMTDYIIMAKDSSYMFVTGPEVIKAVTHEEVTMEDLGGAM
                     THNAKSGVAHLIAENDEQALMMIRELLGYLPSNNMEDPPVESCTDSITREDLSLQTLI
                     PEDPNKPYDIKELITTVVDNHNFFEVQPHYAPNIVVGFGRLDGRSVGIVANNPAHLAG
                     VLDISSSVKGARFVRFCDAFNIPIITFVDVPGFLPGTNQEFGGIIKHGAKLLYAYCEA
                     TVPKITVITRKAYGGAYDVMSSKHIGADVNFAYPTAEIAVMGPEGAVNILYKKENLNE
                     EGRKAKVEEYKTTFASPYKAA"
     assembly_gap    11163..11262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11263..12718)
                     /gene="accC"
                     /locus_tag="PKZ74_03190"
     CDS             complement(<11263..12718)
                     /gene="accC"
                     /locus_tag="PKZ74_03190"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPM91789.1"
                     /translation="MIRKILVANRGEIAVRVMRSCREMDILTVAVYSDADGGAMHVRY
                     ADEAYHIGPAPSSESYLKIDKIIEVAKRCGAEAIHPGYGFLSENAAFSDRCKEEGIIF
                     IGPSGDSIRSMGDKITARKNMIAAGVPVVPGTTEPISDEDKALSVIREIGLPVMIKAS
                     AGGGGKGMRLVKEENEILNAIRAARSEARSAFGDDSVYIEKYIESPHHIEFQILADNH
                     GNTIHLFERECSVQRRHQKVVEETPSPLLTPSVREEMGKFAVAAARAVNYSGAGTIEF
                     IVDESLNYYFLEMNTRLQVEHPITERVVGVDLVKEQIRIANNEVLALKQEELKQRGHA
                     IECRIYAEDPDRNFMPSPGVIKHITEPHGLGVRCDGYVYEGYEIPLYYDPMISKLICW
                     GKTRDEAIARMKRALYEYKITGVKTSIRLLVRIMDTPDFKEGKYNTHFIEKNRDLLFT
                     MPPINPRVEDVAIMTVFMDYLAKVENFQTKVVTNK"
     gene            complement(12826..13731)
                     /locus_tag="PKZ74_03195"
     CDS             complement(12826..13731)
                     /locus_tag="PKZ74_03195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM91790.1"
                     /translation="MYPDLSDILHQVFGIEWLHGLSTYKFFLVSAFAAAAVIVYLELR
                     RKEKVGEVPMKSSRETLIAILMAVVFGMLGARILSALEYLDEFREKPGFSFFIQNGQS
                     FYGGLILASAAIYFYARYKKVDPVHGLDIAAPAIAIGYAVGRLGCHLSGDGCWGVENS
                     TAKPGLLGFLPDWAWACSYPHNALQKGVLMPGCVTDYCHVLPHPVYPTSLYESVFSFI
                     VFSLLWSVRKKVNVTGSLIVIFILLNASFRFLIEFIRINPKYNFLGCHFSQAQLISIL
                     LFFITLTGYYYFRRRSQKQKKQTSF"
     gene            complement(13737..14174)
                     /locus_tag="PKZ74_03200"
     CDS             complement(13737..14174)
                     /locus_tag="PKZ74_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM91791.1"
                     /translation="MKKLFSILLIFVAWMMLFSADTDDELCTKKEEDPCENPITPIAS
                     FTWDRSEIYPGDIVNFSYTGTDDPTAYSWNFHVSANPLTADTRFVSVIFTTAGTREVT
                     LRVKGECAWSSTKMKSVVVRPFPEGRAGHVIPADTNNVLILTP"
     gene            complement(14251..14628)
                     /locus_tag="PKZ74_03205"
     CDS             complement(14251..14628)
                     /locus_tag="PKZ74_03205"
                     /inference="COORDINATES: protein motif:HMM:NF039669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HPM91792.1"
                     /translation="MKKAVILFITVITAAACTSVKNAGSIDLDTSDSTVYEITIIDNQ
                     FDNWYLMNFSPAGDRSNEYYRSMNNLAVSNWNQYYNSGRYKRVIDSYIDYWPTVDYGM
                     EVNRKLYWYFKYIEESSGIRLSQ"
     gene            14739..15419
                     /locus_tag="PKZ74_03210"
     CDS             14739..15419
                     /locus_tag="PKZ74_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91793.1"
                     /translation="MKNSNLIILSILAVTLSLNLSAQKVCTVLKAKIATKYEGKCKNG
                     LAHGKGVATGVDTYEGQFSKGLPEGHGTYSWANGDIYVGDWEQGLRHGEGTMTLKSAG
                     NDSTLTGLWENDKYSGPLPPKPVVLTTMYVDRHKFTKMGKVQHRVLIDFYQNGMRNLE
                     IQNLMPSCSSGNITRLGESFGFENIIFPVTIKVSYTTWNKAHTQMINPIFEFLIADPG
                     DWVVSIHN"
     gene            complement(15432..>16550)
                     /locus_tag="PKZ74_03215"
     CDS             complement(15432..>16550)
                     /locus_tag="PKZ74_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91794.1"
                     /translation="CLHSGHWNFSSFIVCLSLRGQFIDSGNFIVVYSKASGPPWSVTR
                     YIYAQRLDLHGNLKWDQEAPVSALAGPPAWDNFNLKEDGQGGFFIAWHEDREQDKIME
                     SYVQHVDPDGNFRFPDNGLKVGDRPMSHKYYPVVAGQNAANEIFVFWVQMNYEVMMKD
                     AKGDESLWGQRIAADGTLLWSEEGTEIIPTGDFYILSDCDLQDNISYIFYNTIGNDPE
                     DIRIRACSVDDKGNPAWESDYLSISEVPRQVIAPVLSDFADGQWVMFWSDSRVPTLDL
                     YAQNINQDGTIGIPTGIVDPDPRKVSVYPNPCHDRFHIYQEKYNRIGLSDLSGRIIYD
                     KEIKAGTSSFDISSLTPGIYILSLSGPDGQLSRKLAIR"
BASE COUNT         4083 a         4286 c         3990 g         3991 t
ORIGIN      
        1 tcggccagtg attcgcggca tccatagagg ttatcgaatt tcgatttggt gaccgaatcg
       61 ttgacattga tagccgggac gagcaattca ccccgctcct gcatctggta gagccggtgc
      121 acgcctgtcg ttgtttcttc ggaaacacct ctccattcgc tggtggttct ttgccacttc
      181 cggttatcct cgacatagac tttacgcaaa agatccataa tggccgtctc atcctggctt
      241 tcgactttcc tgttcagcaa cgaaggctct ttctcggcct gtacccccca atgaagcagc
      301 aaagtggcat caccgccatc gtcgacaacc agatgcgggc ctttgccgcc ggggaacgaa
      361 agggcctggt aggtgcacca ccaatattcg tcgagcgttt cgcctttcca ggcaaagacc
      421 ggtataccgc gctgtgcaat ggctgcagcc gcatgatcct gggtcgaaaa tatattgcag
      481 ctggcccatc ttacatctgc tcccaattca gtaagggtct cgatgagcac ggctgtctgg
      541 atcgtcatgt gaagagaacc ggagatcctg gcccctgcca gaggtttctg cggtccgtat
      601 ttctcgcgga gcgacatcag tcccggcatc tccttttcgg ccatcccgat ctcttttcta
      661 ccccattcag ccaggctgat atcttttact ttatatttca aatttgtatc tattaccagg
      721 ttttccatat gtaaaaattt gaggctgcaa aggtacgaaa attgtcaatg tggacgaatc
      781 ctagtacttt tggatttgat aatcgtaacg gcatggctga atatcttcgc aggcaaattg
      841 gaaatcattc gcttctggcg ttatggttga ttaccgaaag cacagaagaa cttgaagctg
      901 ccatttcact ccgggccgaa gagaagaaac tctatgaatc ctttgtggcc gaaagccgca
      961 ggaagcaatg gctggcttac cgtctcctca tccgggacct tctcaggccc gacgattttc
     1021 cggtggagta tgacgagacc aataagcctt accttgccgg aagcaattat catatttcgg
     1081 tcagccattc cggcgatatg gcggccgtca tcatcagccg caaatcaagg gtcggaattg
     1141 atatcgaaaa gatcaggccc agggtcggca aggtgaagga acggtttctc agcctgcccg
     1201 agcttgaccg gattggtaac gatagcggtt ttagccggct gacgctggcc tggtcagcca
     1261 aagaagcgat ctataagttg ttcgggcaaa gagacctgga tttcagggaa aacatctgcc
     1321 tgcatctgcc cgatcagccg ggagaaccgt ttgatggcga tatcatcctg gattccggta
     1381 ttacccggtt ccggatcgaa aatgaactca tcggggatta tatcctggtt tatgtcgaag
     1441 agccggcgcc gtgacagatt cccggcaggc agtataaatt tttatacttt tgcatcagag
     1501 cagcaggaaa tgaccatcta ccagaaaatc accggtaaaa tacccggaaa ggcgtttgcg
     1561 gttttgatcg atcccgatca gtaccacacc ggtggcattg acaacattgt caagatcgcc
     1621 tccgacagcc gggtcgattt tatcctggtc gggggcagcc tgctgaccaa cgaccacctg
     1681 gatgagacac tggaaagaat caggtcactg gctgaaattc cggtcatcct tttcccgggc
     1741 agcatcatgc aagtcagcgg caaagccgat gcggtcctgc tgttatcgct gatttcaggg
     1801 cgtaatgccg atctcctgat cggtaaacac gtgatcgccg caccttattt aaaagaaagc
     1861 aaactggaaa tcctcccaac cggatatatg cttattgaaa gcggcccgct gacaacagcc
     1921 cagtacatca gcaacaccct gcccataccg catgccaaag acgacattgc cgcatgcacg
     1981 gccatggccg gcgaaatgct cggcttacgg ctgatctaca tggatgccgg ttcaggcgcc
     2041 gaaaatcccg tatcgccctc catgatccgt aaagtcaggg ctgtaatctc cgtcccgctg
     2101 atcgtcgggg gcggaatccg gacaccggaa caagccatcg tttcctgcca ggccggcgct
     2161 gatattatcg tggtgggcaa tgccatcgaa aaagacccct cctgcctcaa agaaatcgcc
     2221 catgccatcc attcctgttc agaactttaa tctcaccggc ggatttaccc gtcggagcat
     2281 catatcgcaa taaataatac cgaatatgaa aaaaaacatc tccctgattc tcgttttcct
     2341 tttcgccaca tccctggcat ttgcgcaaaa aaaactgacc atcgaagacg tcagtggcat
     2401 gaaccgctcg gtttatccgt catcgatgag aaacctgcaa tgggtgcccg aaagcaaccg
     2461 cttttcgtac atggagaaca atgccctcct cacccgtaaa gccgacaaaa cagttcccga
     2521 tacactggtc aggttatccg aaatgaaccc ggcactgaaa aaagcatcat tcgacaccct
     2581 cgcaaggttt cctttcatca gctgggaaga cgaatcttcc ttcctgttta cgaacaacca
     2641 gaaagtgtac cggtattttg tcaacagccg gtctgtttca tttgtcaatt cctacccgga
     2701 agaagctgaa aatattgatg caactggcaa tatgaaaaag attgcctata ccgtcgataa
     2761 taacctctat ttagctttgg agggaaaaca ggttcaactg acacaggata ccctttcggg
     2821 tattgttaac gggcagagtg ttcaccgcaa tgagttcggg atagaaaaag ggaccttctg
     2881 gtcgccggaa ggcaattacc tggcttacta caggaaggat gaaacgatgg tgaccgatta
     2941 cccgctggtc aacaccacca cgcggattgc agagctggaa aacatcaaat acccgatggc
     3001 cgggatgacc agcgaacaag tgacgcttgt tgtttacaat ctggctgatg gcacccgttt
     3061 gaccgtgaaa accggcttgc ctgccgatca gtaccttacc tcggtgacct ggagccccga
     3121 tgaacaagac ctctacatcg ggattctcaa caggggacag aatcacctga aattgaaccg
     3181 ttatagcgcc actaccgggg agtacgaggc caccctcttc gaagaaaaaa gcgataaata
     3241 tgtcgagccg ctgcatccgc tctatttcat tgattcgcag cccgaccggt tcatctggat
     3301 gagccagcgc gatggatacc gccatatgta cctctattcg accgacggaa ccctgctccg
     3361 ccagctgacc agcggcccat ggatcgtcac ctccttcctg ggaaccgacc cgaaagggaa
     3421 cagggcgttt ttcctggcca cccggaacag cccgcttaac gaagatattt tcgccgttga
     3481 tttcaagacg gcttctatcc agcccgtctc ggccaacacc ggtgttcaca acccggcgct
     3541 cagccccgat gccaggtaca tcctggatgt ttacagcgat accagcgtct gccgggaata
     3601 caacatcatc gactcgaaag ggaaaatgct ccaggtcatt tcgcgcagcg acgacccact
     3661 ggatgactat gtgaaatgcc agagcaagat attccagctc ccggccgacg acaacacccc
     3721 gttgttctgc cagatgatcc tgccgcatga tttcaacccg gcaaaaaagt acccggtcat
     3781 tgtgtatgtt tacgggggtc cccatgcaca gcttgtcaac aacacctggc tcgggggaac
     3841 cagcctgttc aattactacc tcgcgcagca gggctatatc attttcacgc tcgataacag
     3901 gggttcggcc aaccgcggca gggacttcga acaggctgta ttccgcaacc tggggacgct
     3961 tgaagttatt gaccagatga ctggtatcag atacctgaaa agcctgccat atgtcgaccc
     4021 ggagaggatc ggcgtaaccg gatggagcta cggcggattc atgacgattt cgctgatgct
     4081 tcgccagccc ggtgtattca aagtgggcgt ttgcggaggg ccggtgaccg actggaaata
     4141 ttacgaagtg atgtacggcg aacggtacat ggataccccg gaggaaaatc cggacggcta
     4201 tcgtgaagcc agcctgctgg agcgcgccgg agaactgaag ggagatctgc tgatcatcca
     4261 cggcacctcc gatccgacag tggtctggca gcattcgctt gccctgatcc agcgctttat
     4321 cgaggaaggg aaactggtcg attattttgt ttacccggga catggccatg gcgtgggcgg
     4381 caaagaccgg ctgcacctga accggaaaat cgaacaatat tttatcgatc acctatgatc
     4441 cggtggataa tcctgccggt catcctgttt ttctcagtat caggatattt ctccgccacc
     4501 gcccagatcc tgctggccga cagcggcctc tcggattacc ggatcgtgat ccccagcaaa
     4561 ccttctgccg gagagtcgaa agccgccttg cagtttcaat cggttttcag ggaaatatca
     4621 tcagtgacgc tgccgatagt ccccgactcc cggccagact cggaaaagga aatagtcatc
     4681 gggaacagca cacgcctgga aaagattaag tccaaaagcc gttttaaaga tgatgaagat
     4741 gatggattcc tgatccatac tgaccaggct aagcttttca tctgcggagc aggcgacaat
     4801 ggattatttt acggggtttg ctcattcttt gagaattacc ttggttaccg gtactacagt
     4861 ccttcggtaa aacatcatcc tatacttaac aaggtcataa ttaataagat agacgacagg
     4921 caagtccccg tcttcacttt cagggagatg cttttccctg cccggagcga ttcggcttac
     4981 aggcaatggc ataagctgga tacccactcc gacggctcct ggggtatgtg ggtccatact
     5041 ttcgatgacc tggtcccggc tgctaaatat tttgacgaac acccggagta cttttcggaa
     5101 ataaacggga acagggtccg cgacgggcag ttgtgccttt ccaatcccca agtatttgac
     5161 ctggtgattg aaaatctccg aaaaaaaatc attgcacggc cggaagccct ttactggtcc
     5221 gtttctcaaa acgacaactt cctggcctgc cagtgcgcat cgtgtgattc ggttgcaaaa
     5281 aaattcggcg gccagtcagg attgatgatc tggttcgtga accgggtggc ggaactcttc
     5341 ccggataaga tcatctccac acttgcctac cagtacactc gtcatgctcc ggaaaatatc
     5401 cggccactgc cgaatgttaa catcatgctg tgcagcatcg aatgcaaccg gagcaaaccg
     5461 ctccgcaatg atccttcaag cgctgacttt gtgaaagatg taaaggactg gacccggctg
     5521 acggagaata acctggtatg ggattatgtt gtacaattcc gcaactacat cagcccgttc
     5581 ccgaatcttc gtgtcctgca gcccaacctg gagttttttg ccggaaaagg atgcaaactc
     5641 atgttccagc aaggcagcgg aagctcactg tccgaatttt ccgacctgcg ggcctatatg
     5701 atcgccaaac tgctctggga tccttatgcc gatgccgatg ccattatgaa agatttcatc
     5761 tggggctatt atggcgatgc ggctccccac atcctcgact atatcggaat cctccacgat
     5821 gagttggaat catccggcgg agacctggtg atctatggtt ttccatacac cggcgttcat
     5881 tccttcctga aaccattcct gatccccgct taccggaaat gcttcgatga agcggaaaat
     5941 gccgtgaagg ataatcccgg actgctcgca cgggtccact atgcccgtct tccccttgat
     6001 tttgccattc tcgacctctc cctgcaccag gtggatcatg acctgacctg gtttacagga
     6061 gacggaatga atttaacggt gaggcaagat atgatcaacc tgctcgatac ctttgtatac
     6121 cgttgtaaac gactggatgt gaacaccctt aacgaacaat cttattcgcc ggaagaatac
     6181 cggcttacgg ttaaaaaata tttgctgaaa acctccgaaa ggcatatggg catcgggaaa
     6241 gcggctgata tcctcaccga atacagcgcc aaatatgagg caggaggcga aaatgccctc
     6301 accgacggca tcaggggaat taatgactac cacttcaact ggctgggctt tgagggcaac
     6361 gatctggagg ccattatcga tctggaagag gagaaaacac ttgcttccat cagtgccgat
     6421 tttctccagg acatccgctc atggatcttt cttccgaatt cattttcagc atattactct
     6481 ttggatggga tcatcttcca tgaaatcgga acagatccga atatcacccc tgacaataaa
     6541 accggagcct ttataaatac attcacggtg ccggccgatg gtatccgggc ccgctacgtc
     6601 aaagttgttg ccgaatcgat gaaaacctgc cctgaatggc atctcggcaa aggcaaaaat
     6661 tcctggatct ttatcgatga aatcgttata aaatgaacaa actttgtatg aagtccttac
     6721 ggaaattatc ccccctgttc gggcttttat ggcttgtaac tgcatcttac cccctgttgg
     6781 cacaatccgt aaaagtcatg tctttcaata tccgtttcga taatccggcc gatgagaaca
     6841 attgctggga aaacaggcgt aaactggcaa ccctgacgat ccggaatgtc gcacccgatt
     6901 tcatcgggat gcaggaggcg ctggaaaggc aggtgcttta tctcgatgca tcccttccgg
     6961 gttacgatta tatcggcgtt ggcagggacg acggcaaaaa agcaggcgaa tatgtcccgg
     7021 ttttttaccg gaccgatcgg ttcacccttc ttgaccaggg ccacttctgg ctgtcggaaa
     7081 cacccggcga taccggcagc gtgggatggg atgccgctct cccgcggatt tgcacctggg
     7141 gtaaattccg ggaaacagag accggcaggg agatcctttt cctgaatacc catttcgacc
     7201 atatgggcgc aaaggcacgc gctgaaagcg ccaggcttat cctcggtttt atcggtgaga
     7261 atatgcacac tcttcccgtg atccttaccg gcgatttcaa ctgctccgcg gatgaagaac
     7321 cttaccggat tctgacgcag ggcaccgggt tgtcggatgc ctgcctggct tcaggcgaca
     7381 cgaataagtg ttcagaaggg actttcaatg gttttgggca tgagcagaac ccgcaacgga
     7441 tcgattttat tttctttaat gaacaattca ggaccaggtc gtacgaagcc aggaagatca
     7501 cctataacgg catgtttgtt tccgaccact ggccggtgct ggctcagctg gaatgcagcg
     7561 aaaagggagc ccgccctgac gagcgcagcg aggaaggacc cgcaggcaga ggtcaggggg
     7621 atacgacaat cgttcagacg ttcactttcg attccatagt gacgagaaga gctgagtttc
     7681 agtttcccgg caaggaaaag cgttatgaaa agatcctgat gtattacacg ctgaagtgtg
     7741 attcgctgac accgcacgac caatacccct gcggggaatg ggattatacg acatacgtca
     7801 aagtattcgt cccgacaggc cgttacgatt ccttgttcta cacccggacc gattcgctcg
     7861 gaaatgtggt ccgttcgtgg ttcggtgaat ccattgaagt cagggagcct tttgagatcg
     7921 gccggttcat cacaccttac ggaaagcggc tcgacctggg cctgaacgga tttacctgga
     7981 tttatgatgt gaccgattat gagccgttgc tacacggaac ggttgatctg aaggcaggca
     8041 acggccagga actgctcgac ctgcggttcc ttttcatcga agggattccg ccgcgggatg
     8101 tggtttcggt caggaatatc tggcccgaag gttcttatac ctataaagaa ttagcggatg
     8161 atgcaaagct gcagactgtc aggcaaaaac tggcggataa tgccagcacc ttcagcatca
     8221 gggcccggat ttccgggcac ggccatttcg ggccattcaa ctgctgcgag tgggatccga
     8281 agacacatac ttacctggta aacggggaga accggttcga ctggaaagta tggcgcgatt
     8341 gcgggatgaa cccggtccat ccgcagggcg gtacctggca gttcgaccgt gccggctggt
     8401 gccccgggac attcgtcgac acctatgact ttgaaataac cccattcgtc gaacccggcg
     8461 aagaggtatc gattgattac ctgatacagc cgtacgatac ggccaacggc gaagagggag
     8521 gcaatttcga aatggcgatg cacctcattg aatattcctc accaaattat aaactcgacc
     8581 tggaactgac cgacatcctt gcgcccggaa accgtcatga gtaccgaagg atgaatcctg
     8641 tcagcctgaa cccggtgatc aggatcagga gcctcggcac cgacactgtc agaaatatca
     8701 ccttttttta cgggctggag aaaggggaaa agatcaccta cgactggcag ggcaaacttg
     8761 gtttcatgca atcggccgat attatcctgc ctaagccatc ctggcaggga gcggatgata
     8821 aatcaatgtt catcgctgaa ataattacgg tcaatggctt gcaggatgag agggaaagca
     8881 ataacaccca ggtttcggcg atcgtcaaac cggttatcct cccggataaa tttatcatcc
     8941 atgtcaaaac gcagggcttc gggagggccg ccgacaacgc gtggaaaatt aaagacgaaa
     9001 ccggcagggt cgtttacgaa aggctggatt ttctcgacga tacggtttat gacgatcctg
     9061 tcagcttgcc acccggcgct tacgacttca ctttcaccga caagaacgag gacgggctga
     9121 tccgccactg gtggctccac tgggaggatc ccgggaaagt aggccagaac ggggaattaa
     9181 agatccttgg ttcggatgaa aaggagatca tgaacctggg atacgatttt gcggagaaga
     9241 ggacactgca gtttttcgtc ggggaacccg aataaattga tgatttcttt caaaatcact
     9301 tcatcaattt ctccaggttc tcgatataat cgagtgaatc atcctcataa aattcaacct
     9361 ggggaattat cctgacctgt ttgcggatct tattgcccag cttgtatcgg aattcgggaa
     9421 tgtgcgccct gatctcctca accgtgggtt tcttgtcttt caaagcaaag atgctcaggt
     9481 taaaccgggc caccgaaagg tcttttgtca cgtttacctt ggttatcgtg atcatagcgc
     9541 ccgggaacag cctgcgcatg tcgatctcga aaatatcgcc catctccttt tgcagcagcc
     9601 gggccatctt atgttgtctt gtcgagtcca ttcaagtaat aatannnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnngccgct ttgtaaggac tggcaaaagt ggtcttatat
     9781 tcctccacct tggcttttcg cccttcttca ttgagatttt cctttttata gaggatgttc
     9841 accgctcctt ccgggcccat gacggcaatc tctgccgtcg ggtaagcaaa gttgacatcg
     9901 gctccgatat gcttgctcga cataacgtcg taagcaccgc cgtaagcttt ccgggtgatg
     9961 accgtgatct tcggcaccgt ggcttcgcaa taagcgtaga gtaatttcgc cccgtgcttg
    10021 atgatcccgc cgaattcctg gttggtcccc ggtaaaaagc ccggaacatc cacgaacgtg
    10081 atgatcggga tattgaaagc gtcgcagaac ctgacaaacc gtgctccttt gaccgaagaa
    10141 ctgatgtcga gcacaccggc caggtgagcg gggttatttg ccacaatgcc aacagagcgg
    10201 ccgtcgagcc ggccaaaacc gactacgatg ttcggcgcat aatgaggctg gacttcaaag
    10261 aaattatgat tatcgacgac tgttgtgatc agctccttaa tatcgtaggg tttgttggga
    10321 tcttcaggga tcagggtttg caggctcagg tcctcccgtg taatgctgtc ggtgcagctc
    10381 tctaccggcg ggtcttccat gttgttcgac ggcaggtaac ccagcagttc cctgatcatc
    10441 atcagcgcct gttcatcatt ttccgcgatc agatgggcca caccgctttt cgcattgtgc
    10501 gtcatcgccc cgccaaggtc ttccatcgtc acctcttcat gtgtcactgc cttgatcact
    10561 tccgggcctg tcacaaacat atacgacgag tccttcgcca tgatgatata atcggtcatg
    10621 gccggagaat aaacagctcc ccccgcgcac ggaccaagta cggccgaaat ctgcggaata
    10681 acaccggaag ccatgacatt cctgaagaaa atgtcggcat aacccgccag gctctccacc
    10741 ccttcctgaa ttcgcgcgcc gcccgaatcg ttcagcccga tcaccggggc ccccatccgc
    10801 atcgccaggt ccatgatctt cacgatcttg tcggcattgg cccggctcag tgttcccccg
    10861 aaaacggtaa agtcctgggc gaaaacatac accaggcggt cgtcaatctt gccatatccc
    10921 gtcaccacac catcgccggg tatcttgttc ttgtccatat cgaaatcctt ggcccgatgc
    10981 gtcatgaatt tatctttctc aacaaaggtg cccggatcaa gcagtaattc tacccgctcc
    11041 cgcgcagtca gacgcccggc tttgtgttgc ttatctatcc tttccaggcc tccccccagt
    11101 tcggcctgtt tgtctttttc ttcaaaaatc ctgtattttt cttctaacga cgacatagca
    11161 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttgttt gtcacgactt
    11281 tggtctggaa gttttcgacc ttggccaggt aatccataaa taccgtcata atggccacat
    11341 cttctacgcg cggattgatc gggggcatgg taaataacag gtccctgttc ttttcgataa
    11401 agtgcgtatt gtatttacct tccttgaaat cgggcgtgtc catgatcctg accagaagcc
    11461 ggatggatgt tttaaccccc gtgatcttgt attcataaag cgcccttttc atcctggcga
    11521 tcgcttcatc acgggttttt ccccagcaga tgagtttgga gatcatcggg tcgtagtata
    11581 gcgggatttc gtagccttcg taaacatacc catcacacct gacacccagg ccgtggggtt
    11641 cggtgatatg cttgatcacc ccggggctgg gcatgaagtt acggtcggga tcctccgcat
    11701 agatccggca ttcgatggcg tggccgcgct gtttcagctc ctcctgtttc agtgccagga
    11761 cctcattgtt ggctatcctg atctgttctt tcaccaggtc gacaccgacg acgcgttcgg
    11821 tgatcgggtg ctcaacctgc aggcgggtgt tcatctcgag aaagtagtaa ttcaggcttt
    11881 cgtcgactat gaactcgatc gtgccggcgc ccgagtaatt caccgctctg gcagccgcga
    11941 cggcaaattt tcccatctct tcccttaccg aaggggtcag caaaggagat ggagtctctt
    12001 cgaccacctt ctggtggcgg cgctggaccg aacattcccg ctcgaagagg tgaatggtgt
    12061 tgccgtggtt atcggccagg atctggaact cgatatggtg gggcgattcg atgtattttt
    12121 caatgtaaac cgagtcgtcg ccgaaggccg accgggcttc ggagcgggcc gcccggatgg
    12181 cattcagtat ttcgttctct tccttgacca gccgcatccc ttttcctccg ccgccggccg
    12241 atgctttgat catcaccggc agcccgattt cccggatcac ggaaagggct ttgtcttcgt
    12301 cgctgatcgg ttcggtcgtc cccggaacaa cgggcacccc ggctgcgatc atgttttttc
    12361 tcgccgttat cttatcgccc atcgaccgga tcgaatcgcc cgaagggccg atgaaaatga
    12421 tcccttcctc cttgcaacgg tctgaaaatg cggcattttc ggataggaat ccgtagcccg
    12481 gatggatcgc ctctgcgccg catcgtttag ccacttcaat gatcttgtcg attttcaggt
    12541 agctttcgct cgatggcgcc ggcccgatgt ggtaagcttc atcggcatac ctgacatgca
    12601 tggcgccccc gtcggcgtcc gaataaaccg ctaccgtcag gatatccatt tcacggcacg
    12661 accgcatgac ccttaccgca atttcacccc tgttggcaac gagaattttt ctgatcatat
    12721 ctttatttaa attaatttca aataagcaaa atgattatat gtgtctcatt gttagtccat
    12781 tatcatcttg cggcgattag aaaaggattg taaagataat aatttttaaa atgatgtttg
    12841 ttttttctgt ttttgagatc ttcgccggaa ataataatag cccgtcagtg taataaaaaa
    12901 taaaagaatt gaaattagtt gcgcctggga aaaatgacaa cctaaaaaat tatatttcgg
    12961 gttgatgcgg ataaattcaa ttaaaaagcg gaatgaggca ttcagcagga tgaaaatcac
    13021 gatcaggcta ccggtcacat ttactttttt acgaaccgac cataacaggg agaaaacaat
    13081 gaaagaaaac accgattcgt ataatgaggt cgggtaaacg gggtgtggga gcacatggca
    13141 atagtctgtc acgcaaccgg gcatcagaac ccctttttgg agtgcattat gcgggtagct
    13201 gcaagcccag gcccaatcgg gcaaaaagcc aagcaacccg ggttttgccg ttgaattttc
    13261 cactccccag catccgtcgc ccgaaaggtg gcatccaagc ctgccgaccg cgtacccgat
    13321 ggcaatagca ggagccgcaa tatccaggcc atgaacagga tcaactttct tgtaccgcgc
    13381 ataaaagtag atggcggcag aggcaaggat cagtcccccg taaaacgact gtccgttttg
    13441 gataaagaaa ctgaagccag gcttctccct gaactcgtcg aggtattcca gggcgctcag
    13501 gattctggcg cctaacatcc cgaaaacgac ggccattaat atggcaatca gggtctccct
    13561 tgatgatttc atgggcacct cgcccacttt ttcttttctt cttaattcca ggtaaacaat
    13621 aactgcagct gccgcaaatg cagaaaccag gaaaaactta taagtcgaaa ggccatgcag
    13681 ccattcaatt ccaaaaactt gatggagaat atcgctcaga tcagggtaca accggatcag
    13741 ggggttagaa tcaacacatt attcgtatca gcagggatga catggcctgc tcgtccttct
    13801 ggaaatggcc tgacaacaac cgatttcatt tttgtggaag accacgcaca ttcacctttt
    13861 acacgcagtg tcacttctct tgtcccggca gttgtgaata taacagaaac aaaccgggta
    13921 tctgcagtca gaggattggc actaacatga aaattccagc tgtatgctgt cgggtcatca
    13981 gttcctgtat aggagaaatt aactatatca ccggggtaaa tttcactcct gtcccaggta
    14041 aatgaagcaa taggagttat cggattttca cagggatcct cttccttttt ggtgcagagc
    14101 tcgtcatctg tatcggcgct gaacagcatc atccaggcaa cgaagatcag gagaatagag
    14161 aatagctttt tcatgtcagg caggttattg gtttaatcag gtaaatgtaa ttaaaatcaa
    14221 atcctgccgg aacgactgag aaataaatat ttactgcgaa agccttatac cggaactctc
    14281 ctcgatatat ttgaaatacc agtacagctt ccggttaact tccataccgt agtcgaccgt
    14341 cggccagtag tctatgtagg agtctattac ccttttatac cgcccggaat tgtaatattg
    14401 gttccagttc gacaccgcca ggttgttcat cgagcggtaa tattcgttgg agcggtcccc
    14461 ggccggtgaa aaattcatca ggtaccagtt gtcgaactgg ttatcgatga tcgttatctc
    14521 atacaccgtg ctgtcgctgg tgtccagatc aatgctgccg gcattcttta ccgatgtaca
    14581 ggctgcggct gtgatcacag tgataaatag gatgaccgct tttttcatgg ttttgaattc
    14641 tttactaagt tattaaaaag aaacataaaa tatgccacta ttttaaatgc ttatgaaaaa
    14701 aattataatt ttcgatttta aataaaagcc aaatcctcat gaaaaattca aatttaatta
    14761 ttctgtcaat tcttgccgtc acactcagcc tgaacctctc agcccagaaa gtctgcaccg
    14821 tcctgaaagc caaaattgcc accaaatacg agggaaaatg caagaacggg ctcgcccatg
    14881 gtaaaggtgt cgcaacggga gtggatacct atgagggcca gtttagcaaa ggcctgccgg
    14941 aaggtcatgg aacctattcc tgggcaaacg gagatatcta cgttggagac tgggagcagg
    15001 gactcaggca tggcgaaggc accatgacct taaaatcggc cggcaacgac agtacgctga
    15061 caggtctctg ggaaaacgat aaatattctg ggccactgcc tcccaaaccc gttgtgctca
    15121 ccactatgta tgtcgacagg cacaagttta ccaagatggg aaaggttcag caccgggtgc
    15181 ttatcgattt ttaccagaat ggaatgagaa acctcgaaat acagaattta atgccttcct
    15241 gctcgagcgg taacatcacg cggctcggcg aatctttcgg atttgaaaat ataatctttc
    15301 cggtgactat caaggtgtct tataccacat ggaataaggc acacacacaa atgatcaacc
    15361 cgattttcga gttcctgata gccgacccgg gcgattgggt agtgagtatt cataattaga
    15421 cggcagcagt atcaacggat cgccagcttt ctcgacagtt gcccgtctgg tcctgacaga
    15481 ctgagaatat atatccccgg tgtcagggaa ctgatatcga aagatgatgt tcctgccttg
    15541 atctctttgt cgtaaataat ccttcccgag aggtcagaca gccctatccg gttgtatttc
    15601 tcctggtaaa tatgaaatct gtcatgacat ggattgggat acacggaaac ctttcgagga
    15661 tccggatcca ctattccggt cggaattccg atggtcccgt cctggttaat attctgggca
    15721 taaagatcaa gggtcggaac ccggctgtcg ctccagaaca tcacccattg cccgtcggca
    15781 aaatcggaga gaacgggcgc tatgacctgg cggggaacct cagaaatgga caggtaatca
    15841 gattcccagg ccggatttcc tttatcatcc acactgcagg cccggattcg gatatcctcg
    15901 ggatcattcc cgatcgtgtt gtaaaaaatg tacgagatgt tgtcctgcag gtcacaatcg
    15961 gaaagaatgt aaaaatcgcc cgttggaatg atttccgtac cctcttctga ccacagtaag
    16021 gttccatcgg ctgcaatccg ctgcccccag aggctctcat cgcccttcgc atctttcatc
    16081 attacctcat aattcatttg cacccagaac acaaaaatct catttgccgc attctggccg
    16141 gccactaccg gataatattt gtggctcatc ggcctgtccc ctactttcag cccattgtcg
    16201 ggaaaccgga aatttccgtc gggatctaca tgctgaacat acgattccat gatcttatcc
    16261 tgttccctgt cttcgtgcca ggcaataaaa aagcctccct ggccgtcttc tttcaggttg
    16321 aaattatccc acgccggcgg gcctgccagc gcgctaaccg gcgcctcctg gtcccatttc
    16381 aggttgccgt gcaaatcaag cctttgtgca taaatatagc gggtaaccga ccacggtggc
    16441 cccgaagctt tcgaatagac cacgataaaa ttgccgctat caataaattg accacggagg
    16501 cttagacata caatgaatga actgaagttc caatgtcctg aatgcaagca
//
LOCUS       DAOPNH010000035        16191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3528_length_16191_cov_6.074492, whole genome shotgun sequence.
ACCESSION   DAOPNH010000035 DAOPNH010000000
VERSION     DAOPNH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_16191_cov_6.074492"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..941)
                     /gene="xerD"
                     /locus_tag="PKZ74_03220"
     CDS             complement(39..941)
                     /gene="xerD"
                     /locus_tag="PKZ74_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPM91795.1"
                     /translation="MNWNPYIKGFTSFLRLEKSLSGNSVEAYERDVVKLVEYLDINQL
                     ELQPKDITLAHLQDFVKWVNQLGMSARSQARIISGLKTFFKYLLLENTIRANPAELLE
                     SPRTGRALPDTLNRVEIEQIFDAIDLSTDEGERNRTILEFLYGCGLRVSELTGLKISD
                     IRFNEGFLSVIGKGNKQRLVPVGQKALKQLDIYIHQIRSHIPVQRGFEDIIFLNNRGK
                     GLSRVMIFYIIKDLAEKAGVRKTISPHTFRHSFATHLIEGGADLRAVQEMLGHSSITT
                     TEIYTHLDRDYLRSNILQYHPRAK"
     gene            1028..1216
                     /gene="rpmG"
                     /locus_tag="PKZ74_03225"
     CDS             1028..1216
                     /gene="rpmG"
                     /locus_tag="PKZ74_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPM91796.1"
                     /translation="MAKKSKDQRIQVILECTEHKNSGQPGTSRYITTKNKKNTPERLE
                     MKKYNPILKKVTVHKEIK"
     gene            1225..1377
                     /locus_tag="PKZ74_03230"
     CDS             1225..1377
                     /locus_tag="PKZ74_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPM91797.1"
                     /translation="MAKKVIATLQTQGKEFSKLIRMVRSPKTGAYVFKETMLPNDQVK
                     DFIAKK"
     gene            1452..2411
                     /gene="ftsY"
                     /locus_tag="PKZ74_03235"
     CDS             1452..2411
                     /gene="ftsY"
                     /locus_tag="PKZ74_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPM91798.1"
                     /translation="MGLFSFFSKEKKETLDKGLDKTRQNVFSRISRAIAGKSRVDDEV
                     LDNLEEILVTSDVGVETTLKIIERIQQRVARDKYVGTEELNRVLKEETASLLNESNGD
                     EGFDFGSANLPVPYVIMVVGVNGVGKTTTIGKLAFQFKKAGKTVVLGAADTFRAAAVD
                     QLKIWGERAGVPVITQGMNADPAAVAFDTVKSAHSQGIEIVIIDTAGRLHNKINLMNE
                     LSKIRRVIQKVIPEAPHEVLLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKG
                     GVAIGISDQFKVPVKYVGLGEKIEDLQIFNRMEFVDSLFGEKA"
     gene            2448..3746
                     /gene="rimO"
                     /locus_tag="PKZ74_03240"
     CDS             2448..3746
                     /gene="rimO"
                     /locus_tag="PKZ74_03240"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPM91799.1"
                     /translation="MNSKSRARKINIVTLGCSKNLVDSEMLMGQLKAAKVDVAHESGL
                     PADVVVINTCGFINDAKEESIDTILQYAEAKKAGKVRKVYVMGCLSQRYRKALEAEML
                     EVDGFFGVNDLPQILRELGVDYRNELVGERMLTTPSHFAYMKISEGCDKKCSFCAIPL
                     IRGKHISRPLDELVRETALLAAKGVKEIILIAQDLTSYGIDLYGKRNLRILLEKLSEV
                     EGVEWIRLHYAYPANFPIDILDLIRDNPKICKYIDIPLQHISDPVLRSMKRGVTQSKT
                     LRLLETIRTIIPQAAIRTTLIVGYPNETDADFEALKAFVRQQRFDRLGVFTYSPEENT
                     SSYNIPDNIPEDVKKSRMEEIMEIQQEISLEKNSGKIGSIYKVLIDRKEGENYIGRTE
                     YDSPEVDNEILIRSQQALQIGKFYPVRIDRADFFDLYGSV"
     gene            3758..4483
                     /locus_tag="PKZ74_03245"
     CDS             3758..4483
                     /locus_tag="PKZ74_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPM91800.1"
                     /translation="MKTKIIMGLLLMIPVFSFMCTYGQDAQNVEVKNGFYTEKFGDLN
                     IEGNYSNGQKEGVWITYFPTGYLNKVEEYHQGKKNGLFIEFSSKGEIISQASYKDDKL
                     NGISKKYGRAGKFEWVKSYRMNKLHGLVQMFYDGGKLQEEAWYSHGQRDSITTWYDTQ
                     GNKIMTTNYSEGLFDGISYTYFSNGQVKTETTYAGNVVNGAFKEYDEEGRLRVSGQYE
                     NGIKEGTWFSYDVSGKIVSKEKY"
     gene            4490..6121
                     /gene="pruA"
                     /locus_tag="PKZ74_03250"
     CDS             4490..6121
                     /gene="pruA"
                     /locus_tag="PKZ74_03250"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPM91801.1"
                     /translation="MSKGFYKAPRPYNEPVLSYAPGSPERKAVKNAIADFRSGEIDIP
                     MYIGGEEVRTGERIRISPPHDHRHTLGYYHLGTGEHVLQAINTALAIRPKWQSLTWDQ
                     RAAIFLKAADLISGPYRARLNAATMLGQSKNIYQAEIDAACELADFLRFNVSFMTEIY
                     REQPVSSHGIWNKMEWRPLEGFVYAVTPFNFTSIAGNLPSAPALMGNVVVWKPAGTQV
                     YSANVIMEIFRAAGLPDGVINMIMVDGPVGGKIMSTHPDFVGVHFTGSNTVFNKIWKA
                     IGENVALHNTFPKIVGETGGKDFVMVHNSAHPKAVATALSRGAFEYQGQKCSAASRAY
                     IPRSLWPEIKQMMLDDISSFRMGGVEDFRNFINAVIDEGAFDKLAGFIDRAKTDEGVE
                     IIAGGNYDKSVGYFIEPTVILTKNPRYVTMCEELFGPILTVFVYEDENFDETLDIVDK
                     TSPYALTGAVFAQDRFIIDEMTRRLVNAAGNFYINDKPTGAVVGQQPFGGARASGTND
                     KSGSFLNLLRWVSPRTIKENFDPPQDYRYPFMEEE"
     gene            complement(6143..6325)
                     /locus_tag="PKZ74_03255"
     CDS             complement(6143..6325)
                     /locus_tag="PKZ74_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91802.1"
                     /translation="MKKVVKIIVLLIVIVSLTGLMNSCAIFDESSHAQKLKTFKHNKP
                     LPKKYILDNGYKPIAK"
     regulatory      6451..6665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6749..8668
                     /locus_tag="PKZ74_03260"
     CDS             6749..8668
                     /locus_tag="PKZ74_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91803.1"
                     /translation="MQWMKNSVFIAAFYFMALSAESQHALPDTVLQLDGVTIFSERSG
                     SAKGLLTVSLDSITRDQHPGADLAELIQGFSAAYVKNYGEGTLSTLSVRGTSANHTGL
                     YWNGIKLSPPNIGYVDLSLIQGNFFQDVSIMFGGASPMFGSNAIGGSIQLNNKPVFDP
                     TGLKGRISFSGGSFSRLNSAGNLSLSKGKLYSNTAFSIAGSENDFPYSDLYKKDARMP
                     HAGYFKTGFLQDIAFQLPRNQYIMASAWFQYADREIPPTLLETVSSAIQLDRSWRTML
                     TWKDFNAKSTWEAKAAWIEEFTRYSDSLSGIYSVIRCQTFNAVFEGYYHLFRNASLSG
                     GLDFSSEYADLDYYSSPAGQQKLAAFAAWVQSFSGSSWKLSLNVRQELQSGYPIPFLY
                     AIGAEGRLLKILTGRASFSRNFRAPTFNERYWRPGGNPALLPESSYNAEAGFRIIPLP
                     ENVTTFLDFTGFSSWVDNWILWLPGEGGLWSVENAQRVWARGVEVRLNVTRQFRYIKA
                     NAMGAYTFSASTNRKKLFELDQSFNKQLIYTPVHRFAFRAGAEWQGFSLYLKGNFSSR
                     VYTTRDNSASLSPWFLLDAILAKSVFVTKRFPLMIQLNLENILDTEYSITPYRPMPGF
                     NVLITIALNINEENK"
     gene            8718..9782
                     /locus_tag="PKZ74_03265"
     CDS             8718..9782
                     /locus_tag="PKZ74_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91804.1"
                     /translation="MKKIKYSLLPLAFSFVLWLFSGCGDEPVSPPSVWTGNIFIINEG
                     PFTSGTGTIMAYNRTTGQMSGDLFEPVNGRPIGNIVQSIGVYRNMVWIMVNNSNKVEV
                     ARYGDFSSVITIDSLPSPRYIAFKGDLAYVSSWNNKIFAIDTRSYKIVDEFPTGTGPD
                     EMVIAGDYLFAVNSGGLGTTNTISFINIKQKEVNGEITVGDRPCGITEDKYGKLWVLC
                     TGNGWNGWPSPETDTPGKLVCIDPSTRQIIGEFPFSDPALHPDNLIINGERSVLYYAY
                     PDGIYSVSVDNPALAVEPLVPSTVMYYGLGYDVAEDIIYAADPLDYAQNGIIYRYSAM
                     DGLLIDFFTAGMVPNGFWFN"
     gene            9821..10237
                     /locus_tag="PKZ74_03270"
     CDS             9821..10237
                     /locus_tag="PKZ74_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM91805.1"
                     /translation="MKTKIDCTWKENMAFEADVNGFKLMLDADPAVGGENQGPRPKPL
                     TLVSLAGCTGMDVISILKKMRVEPDYFNVAVEGELTEEHPKYYHKIHLVYEIKGHDID
                     PEKVEKAVSLSQERYCGVSALLKAGAEITHEIRILS"
     gene            complement(10209..10769)
                     /locus_tag="PKZ74_03275"
     CDS             complement(10209..10769)
                     /locus_tag="PKZ74_03275"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPM91806.1"
                     /translation="MKRIVEGGQVKIPGNAKKAGVLVLFYPIMDQPHIVFIRRNEYPG
                     VHSGQISFPGGAYEEADSDIVTTALRESEEEIGVDRSVVRVLGRLTDLYIPPSNFLVS
                     PVVGYSTLRPEFCPDPQEVDRILEISFNAISDSENLTEKQIEIFPGTRITAPCYFIED
                     QIIWGATAMILHELTDVISSVSGFHA"
     gene            10963..11235
                     /locus_tag="PKZ74_03280"
     CDS             10963..11235
                     /locus_tag="PKZ74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPM91807.1"
                     /translation="MVKEKLKPGQDQKELADNEKELILFNDEINTFEFVIDTLIEVCG
                     HDPFQAEQCALTAHFNGKCGVKSGTFHELKPVYDEMISRQLTVSIS"
     gene            11238..11705
                     /locus_tag="PKZ74_03285"
     CDS             11238..11705
                     /locus_tag="PKZ74_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPM91808.1"
                     /translation="MHWVLIATFILIGLVFLVLEILVIPGVGVAGIIGFVLIAVGVWQ
                     SYAVYGLAAGHAVLGGTLVLTVLTLFLSLRGKTWKRISLSTSIDSKVNVIDQKNIKPG
                     DSGKTVSRLAPMGKAIINEEFYEVTTNGDFIDQQTEITVVKIDHNKIIVKRKE"
     gene            11711..12724
                     /gene="floA"
                     /locus_tag="PKZ74_03290"
     CDS             11711..12724
                     /gene="floA"
                     /locus_tag="PKZ74_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613657.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPM91809.1"
                     /translation="MGDSQVFIIVAIGLGAIFAIWLIFYFIPVGIWFKALVSGVRISL
                     LQLVIMRWRKVPVTIIVNSLIAATKAGLQLNRNSLEAHFLAGGNVTRVVNSLISADKA
                     NMKLDFQTATAIDLAGRDVLEAVKMSVNPKVIDTPSVTAVAKDGIQLICKARVTVRAN
                     IKQLVGGAGEETVLARVGEGIVSSIGSAHSHKSVLENPDSISKLVLDKGLDSGTAFEI
                     LSIDIADIDVGKNIGAFLQMDQANADKNIAQARAEERRAMAVATEQEMKAKAEEARAN
                     LIQAEAEIPKAIAEAFRQGNLGIMDYQRYQNIKADTKMRESISEPPSSKSKKGIKDQE
                     KES"
     gene            12740..14929
                     /locus_tag="PKZ74_03295"
     CDS             12740..14929
                     /locus_tag="PKZ74_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPM91810.1"
                     /translation="MISETDLQKISAKYEGLLASAQGLLKKDDRQLLDVAFEFARNVY
                     GDTRHVSGELMICHCLSIARIAAEEMSLGINALIAALFHDIQAIQPAGLEEIKKRFGD
                     PVAAMVKSYSRISELPTGKISLQSDSFRQLFLAVVDDIRVILLKLAHRLRDMRMIDKL
                     PNNYQERYINEVNHLYSPIAHRLGLYRIKKELEDLSMKFSHPGVYQDIARKIRETEAK
                     RNAFINEFISPIQRELFKQGFSFDIKGRPKSIPSIWEKMKKQDVEFEQVFDLFAIRII
                     LNSTAEHEKSDCWKAYSIVTNIYSPNPKRLRDWISTPKASGYESLHTTVKGPNDRWVE
                     VQIRSARMDEIAEKGQAAHWRYKGFGSKEDTERWMLQVRDIIEHPEQIAFDDTVTAVS
                     ESKPGKVFVFTPNGDLKELDAGSTVLDFAFEVHTSVGYSCTGAKVNNKLVPIKQTLEN
                     GDKVEIITSKIQKPKMDWLNFVVTSKAKNKIKRALKEEQFLEAEKGNEILRRKFKNWK
                     IAFIDENIDKLIRKYKLSSSIDLYGLIYNEKIDLVEMKRFLAGEPEKPEESKIPAKDI
                     KPVQTSRGTAQESNEVLFLDTNLNKVNYKLARCCNPQPGDPVFGFVTISRGITLHTQS
                     CPNARQLLTRFGYRKIDVQWKTSPDKNLVRAVINVTGTDRIGIMNEITQIISNDLKTN
                     MISVKIDVRKNIFNGIIKLLVKNTSSLNDLIGRISKVSGVSKVVKID"
     gene            15003..15476
                     /locus_tag="PKZ74_03300"
     CDS             15003..15476
                     /locus_tag="PKZ74_03300"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HPM91811.1"
                     /translation="MKKMRLFTFLIIALSIALVSCDKDNDGNNNGMGGTMTLKHDGSN
                     WSASLAVAAANTNGVLSVTGSDNSANQCNVTLYNIPGPGTYQLGGSMSNPNTGRWTQG
                     LGQNDTYSTMVGQGTGTCTITELTASKVSGTFEFTAKNTAGNMVTISEGSFSADL"
     gene            15661..16050
                     /locus_tag="PKZ74_03305"
     CDS             15661..16050
                     /locus_tag="PKZ74_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPM91812.1"
                     /translation="MKNTPKKFKPTETELEILSILWNKGPATVRDVNDEMSRSKPTGY
                     TTTLKLMQIMAEKGLVEREMDGRSHIYHALARQEDAQVSMLDRLLETAFGGSASKLVM
                     QTLGNHKTTESELKEIKLLIKKLEEEK"
BASE COUNT         4581 a         3561 c         3843 g         4206 t
ORIGIN      
        1 cagtttcatg tttcacgatt caggttagaa ccaccctgct actttgccct tgggtgatac
       61 tgcagaatat tgctccgcag gtaatcccgg tcgagatgtg tgtaaatttc tgttgttgtg
      121 atcgatgagt ggcccagcat ctcctgcacg gcccgcagat cggcgccccc ctcgatcagg
      181 tgagtggcga aagagtgcct gaaagtatgc gggctgatgg ttttccggac ccctgccttt
      241 tcggccagat ccttaataat ataaaagatc ataaccctgg ataagccttt acccctgtta
      301 ttcaggaata tgatatcctc aaacccccgc tggacaggta tatggctccg aatctggtga
      361 atgtaaatat ccagttgctt gagcgctttc tgtcctaccg gtaccagtcg ctgtttattt
      421 cctttcccga taacggatag aaaaccttca ttaaacctga tatccgatat cttaagcccc
      481 gttaattcac tgaccctgag cccgcatccg tacaagaatt ccaggatggt acggttcctt
      541 tcgccttcat ccgtgcttaa atcgatcgcg tcgaaaatct gctctatctc cactctgttc
      601 aatgtgtcgg gcaatgccct gccagtcctg ggtgattcca gcaactccgc gggatttgcc
      661 cggatcgtgt tttccagtaa caggtactta aagaatgttt taagccctga gatgatcctg
      721 gcctgcgacc gtgcactcat tcccagctgg ttaacccatt tgacaaaatc ctgaaggtga
      781 gccagtgtga tgtccttggg ttgaagctcc aactggttga tatccagata ttctaccagt
      841 ttgaccacat cccgttcgta ggcttcaacc gaattaccgg ataacgattt ttccagcctg
      901 agaaacgacg taaaaccttt gatgtatgga ttccagttca aggtcgggta atttttgata
      961 aaattatagg ttattttcaa ataaaattct atctttgcag cccaaaatta aaaaggagtc
     1021 ttttaaaatg gcgaagaaat cgaaagatca gcggattcag gttatactgg aatgcaccga
     1081 gcataaaaac agcggccagc cgggaacctc gaggtacatt accacgaaaa acaagaaaaa
     1141 caccccggaa aggctggaaa tgaaaaagta taatcctatc ctgaaaaaag ttacggttca
     1201 taaagaaatt aaataagtcg agctatggca aagaaagtta ttgcaaccct gcaaacccag
     1261 ggaaaggaat tctccaaact gatccgcatg gtgcggtcgc ccaaaaccgg ggcttatgtt
     1321 tttaaggaaa caatgctccc gaatgatcag gttaaggatt tcattgcgaa gaaataacgg
     1381 gtttatagaa aaccggttcc gaaaagcttt tcccgccgga agagcttttt ttatttaaaa
     1441 atggatacaa aatgggacta ttttcatttt tttcaaaaga gaagaaggaa acccttgaca
     1501 aaggcctgga taaaacccgc cagaatgtgt tctcacggat cagccgtgcc atagccggga
     1561 aatcccgcgt cgatgatgaa gtgctcgata acctggaaga aatcctggtc acttccgatg
     1621 tgggagtgga gactacactg aagatcatcg aaagaattca gcaaagggtg gcacgggata
     1681 aatatgtcgg gacagaggaa ttgaaccggg ttttgaaaga ggaaacagcc tccctgctga
     1741 atgaaagcaa cggggatgaa ggctttgatt tcggatccgc aaacctgccc gtaccttatg
     1801 ttattatggt cgttggtgtt aacggggtcg gaaaaactac aaccatcggt aagctggctt
     1861 tccagttcaa gaaagcagga aaaacggtcg tcctcggcgc agccgacact ttccgcgctg
     1921 cagcggtcga tcagctcaag atctggggcg aaagggcagg ggtacctgtc atcactcagg
     1981 gaatgaacgc agatcccgca gcagtggcct tcgatacagt gaaatcggct cactctcagg
     2041 ggatcgaaat cgtgattatc gatacggcag gccgccttca caacaaaatc aacctcatga
     2101 atgagctctc caaaatcagg cgggtcatcc agaaagtcat cccggaagcg ccacatgaag
     2161 tcctgctcgt tttggatgcc agcacaggtc agaacgctat tgaacaggca agacaattta
     2221 ccgccgctac cgaagtcaat gccctggcac ttactaaact cgacggaacc gcaaaaggtg
     2281 gcgttgctat cggtatttcc gaccagttca aggtcccggt caaatatgtg ggactggggg
     2341 agaagatcga agacctccag attttcaacc ggatggaatt cgtcgatagc ctcttcggcg
     2401 aaaaagcata aacagaaatc caacattctc aactcaaatc tttagacttg aattcaaagt
     2461 cccgggctcg taaaattaat attgtcaccc tgggttgttc caaaaacctg gtcgattcgg
     2521 aaatgctgat gggccagcta aaggcggcaa aagtggatgt tgcccatgaa tccggtttgc
     2581 cggcagatgt tgtcgtaatc aatacctgcg gatttatcaa tgatgcaaaa gaagaatcaa
     2641 tcgatacaat tcttcagtat gccgaagcta aaaaggccgg caaagtgcgg aaagtatacg
     2701 ttatgggttg cctgtcgcag cgttacagga aggcactgga ggcagagatg ctcgaagtag
     2761 acggattctt tggtgtgaat gacctgccgc agattctccg ggaattgggc gtggattacc
     2821 gaaatgagct ggtgggtgaa aggatgctca ccacgccgtc gcattttgct tacatgaaaa
     2881 tctccgaggg gtgcgataaa aagtgttcat tttgtgcgat cccgttgatt cgcggaaaac
     2941 atatctcccg gcctctggat gaattggtca gggaaaccgc tctcctggca gcaaaaggtg
     3001 ttaaagagat cattctgatt gcccaggatc tgacctccta tggtatagac ctttacggca
     3061 aaagaaacct gaggattttg ctggagaaac tctcagaagt tgagggagta gagtggatac
     3121 ggcttcacta tgcctatcct gcaaatttcc ccattgatat cctcgacctg atccgggata
     3181 atcccaaaat atgcaaatac atcgacatcc cgttgcagca tatcagcgac ccggttttgc
     3241 gttcaatgaa aaggggtgtc acacaaagca aaaccttacg cttgcttgaa accatccgga
     3301 ctataattcc gcaggccgcg attcgtacca cactaattgt cggttacccc aatgaaaccg
     3361 atgctgattt cgaagcgctg aaagcatttg taaggcagca aagattcgac cgcctgggcg
     3421 tttttaccta ttcgccggaa gagaatacat catcgtacaa tattcctgat aatattcctg
     3481 aagatgttaa aaaatcccga atggaagaga tcatggagat tcaacaggaa atttcactgg
     3541 aaaagaattc aggtaagatc ggaagtattt ataaagtgtt gatcgataga aaagaaggtg
     3601 aaaattatat cggacggact gagtacgact cccccgaggt cgataatgaa atcctgatcc
     3661 gttcgcagca ggccttgcaa ataggaaaat tttacccggt cagaatagac agggcagatt
     3721 ttttcgacct ttatggttcg gtttaaattt tttcatcatg aaaacaaaaa tcattatggg
     3781 attgttatta atgatcccgg ttttttcatt tatgtgtaca tatggtcagg acgcgcaaaa
     3841 tgtggaggtt aaaaacggtt tttataccga aaagttcggt gatctgaata ttgaaggcaa
     3901 ttattctaac ggacagaagg aaggtgtttg gattacctat tttccaaccg gataccttaa
     3961 taaagtagaa gaataccatc agggtaagaa gaatgggctc tttatagagt tcagcagtaa
     4021 gggcgagatc atcagtcagg cttcttataa ggatgataaa ctcaacggaa tcagtaagaa
     4081 atatggccgc gcaggtaagt tcgaatgggt caagagttac aggatgaata aattacatgg
     4141 attggtccaa atgttctatg atggcggaaa gttacaggaa gaggcatggt attcccatgg
     4201 ccagcgcgac agtattacaa catggtatga tacccaggga aataaaataa tgacaacaaa
     4261 ttattccgaa ggacttttcg acggaatcag ctatacctat ttctccaacg gccaggttaa
     4321 gacagaaacg acttatgccg gaaatgtggt taacggagct tttaaagaat atgatgaaga
     4381 aggaagatta agagtgtcgg gtcaatatga aaatggcatt aaggaaggta cctggttttc
     4441 ttatgacgtc agtggtaaaa tagtatcaaa agaaaaatat taaacgaata tgtcaaaagg
     4501 attttataaa gcgcccaggc cctacaacga accggtttta tcttatgcgc ccggttcgcc
     4561 ggaacggaaa gctgtaaaaa atgcaatagc ggacttcagg tccggggaga tcgatattcc
     4621 aatgtatatt ggcggagaag aggtcagaac cggcgaacga atccggataa gtccgcccca
     4681 tgatcacagg cataccctgg gttattatca tctaggcacc ggggaacatg tccttcaggc
     4741 aatcaatacc gcattggcta tcaggcctaa atggcagtca ctcacctggg accagcgggc
     4801 agccatcttt ctgaaagcag ccgacctgat cagcggtcct taccgggccc ggctcaatgc
     4861 ggctaccatg ctcggccagt caaagaatat ctaccaggca gaaatcgatg cggcatgcga
     4921 gcttgctgat tttttgcgct ttaatgtaag cttcatgacc gaaatttacc gggagcagcc
     4981 ggtttcatcg cacggaattt ggaataaaat ggaatggagg ccgctggagg gttttgtcta
     5041 tgcagtcacc ccgtttaatt ttacctctat cgccggaaac cttccctcgg cgcctgcact
     5101 gatgggtaat gtggtagtct ggaaaccggc cggcacacag gtctattcag cgaatgtgat
     5161 catggagatc ttccgcgccg ccggattgcc cgacggagta atcaatatga tcatggtcga
     5221 cggtcctgtg ggcgggaaaa ttatgtcgac acatcccgat ttcgtagggg tgcattttac
     5281 cgggtcgaat acggtcttta acaaaatctg gaaagccatc ggcgaaaatg tggctctgca
     5341 taataccttt cctaaaatcg taggggagac cggcggcaaa gattttgtca tggttcataa
     5401 ctccgctcat cctaaagcag ttgcaacagc attaagccgg ggcgcctttg aataccaggg
     5461 gcagaaatgt tccgctgcct caagggccta tatcccccga agcctatggc cggaaataaa
     5521 acaaatgatg ctcgacgata tttccagttt ccgcatgggt ggggtggaag atttccgaaa
     5581 tttcatcaat gcggtgatag atgaaggtgc ttttgataag ctggccggat ttatcgacag
     5641 ggctaaaacg gatgagggtg ttgaaatcat tgccgggggc aattatgata aatctgtcgg
     5701 ttactttatc gagccgaccg ttatcctgac gaagaatccc cgttatgtga cgatgtgtga
     5761 agaacttttc ggcccgatcc ttaccgtttt cgtttatgaa gatgagaatt ttgatgaaac
     5821 ccttgatatt gttgataaaa cttcgcctta tgccttgaca ggagcggttt ttgcacagga
     5881 ccgcttcata atcgacgaga tgacccggcg tctggtaaac gctgccggca atttctatat
     5941 caatgacaaa cccaccggcg ctgtggtcgg acaacaaccg tttggcgggg cgagagcttc
     6001 agggacaaac gataaatcag ggtccttcct gaaccttctc aggtgggtat cccccagaac
     6061 catcaaggaa aattttgatc cgccgcagga ttatcgctat cccttcatgg aagaagaata
     6121 gccggatacg gttttaaggg aactatttag cgatcggttt atatccgtta tcgagaatgt
     6181 atttttttgg aagaggctta ttgtgtttaa atgtcttaag tttttgcgca tgtgaactct
     6241 catcaaaaat ggcgcaggaa ttcatcagcc cggtcaggga aacaataaca ataagcagga
     6301 caatgatttt aacaactttt ttcattttat taatcctcaa tttttttttg atcgggtcat
     6361 ttattttttg agggtataaa tttataaaaa taacgttcat aacgtggtga atattttttt
     6421 ttcagggata aaccggtaat cattatccct ttatctttgt gatgtgacgg agcccctgac
     6481 attctaccgg atacagggga taatagggaa ccgggtgaaa atcccggaca gtgcccgctg
     6541 ctgtgaatcc cggccttgag ccaaaatcac cggcaacctc gtgtcactgt cccgataaca
     6601 agggatggga agactgccgg acgatgggat gagtcagaaa acctgccgcc attttaaaat
     6661 atgtctctgg tttccgggtc agaagccggg ctgaaaaggt tcagatttat ccactttttt
     6721 agcttctaac ccaaataacc ggtaattcat gcaatggatg aagaattcgg tatttatcgc
     6781 ggcattttat tttatggcat tatctgctga aagccagcat gctttgccgg ataccgtact
     6841 gcaactggat ggggttacta tattttctga aaggagcgga tcagcaaaag ggctgctaac
     6901 cgtttcactg gattctataa cccgggatca acacccgggt gcagacctcg ccgaacttat
     6961 ccagggcttt tctgcagctt atgtgaaaaa ctacggagaa ggaaccctct ctacattatc
     7021 ggtcagggga acctcagcca atcataccgg gttgtactgg aacggtatca aattgtcgcc
     7081 gcccaatatc ggctatgttg acctgtcgct gatccagggg aatttcttcc aggatgtgtc
     7141 catcatgttc ggaggggcca gccccatgtt cggcagtaat gccatcgggg gaagtattca
     7201 actgaataac aagcctgttt ttgatcccac agggctgaag ggcagaataa gtttttcagg
     7261 aggaagcttc agccggttga acagtgccgg aaatcttagt ttatccaaag gaaagctata
     7321 ttcaaatacg gcgttttcaa ttgccggatc agaaaatgat tttccctatt ccgatttata
     7381 taaaaaggat gcccggatgc ctcatgcagg ctactttaag acagggttcc ttcaggatat
     7441 tgcctttcaa ttgccgcgca atcagtatat tatggccagc gcatggtttc agtatgccga
     7501 tcgcgaaatt cctccgactt tgctggagac cgtatcttct gccatccagc ttgaccgttc
     7561 ctggaggaca atgctcacat ggaaagactt taatgccaaa agtacgtggg aggccaaagc
     7621 tgcatggatc gaagaattta ccaggtactc ggattcactg tcaggcatct attcggtcat
     7681 tcggtgccag acgttcaacg cagtattcga gggatattat catttgttca gaaatgcatc
     7741 cctttccgga ggactggatt tttcaagtga atatgctgac cttgattact attcgtctcc
     7801 tgccggtcag caaaaacttg cggcttttgc cgcatgggtg cagtcctttt ccggctcatc
     7861 ctggaaattg agcctgaatg tgcgccagga attgcagtcg ggctatccta ttccttttct
     7921 ttatgcgatc ggcgccgagg gaaggttatt aaagattctg accggcagag cgagcttttc
     7981 cagaaatttc agggccccga catttaatga aaggtactgg cgaccgggtg gcaatccggc
     8041 gcttttaccg gaaagcagct ataatgctga agcgggtttc cggatcatcc cattaccgga
     8101 aaatgttaca accttcctgg atttcaccgg tttcagctcc tgggttgata actggattct
     8161 gtggctcccc ggtgaaggcg gattatggtc ggttgaaaac gcacagcggg tctgggcaag
     8221 aggagtggag gtccgtttga atgttacccg gcaatttcgt tacatcaaag ccaatgctat
     8281 gggtgcgtac acgttttctg cttctacaaa ccggaaaaag ctattcgaac tcgatcaatc
     8341 atttaataaa caactgatat acacacctgt acatcgattt gcctttcggg ccggggccga
     8401 atggcaaggg ttcagccttt acctgaaggg gaatttcagc agcagggttt atacaacccg
     8461 ggataattcc gcgtcccttt ctccatggtt cctgctcgat gcaatcctgg ccaaatctgt
     8521 ttttgttaca aaaagatttc ctctgatgat tcaattgaac ctcgaaaaca ttctggatac
     8581 tgaatatagc attactcctt accggcctat gcccggattt aatgtgttga tcaccattgc
     8641 tttaaatatc aatgaggaaa ataaatagaa cctttataat agatgtagaa gtaaacaaat
     8701 cagaaaaaat tattatgatg aaaaagatta agtattctct ccttccattg gctttttctt
     8761 tcgttttatg gctgttttcc gggtgcggcg atgaaccggt gtcacctcct tcggtttgga
     8821 ctggaaatat ttttattatc aacgaagggc ctttcaccag cggcaccgga accatcatgg
     8881 cctataaccg gacaaccggt cagatgagcg gcgacctttt tgaacctgtc aatggccggc
     8941 cgatcggcaa tatcgtacaa tccatcggcg tttaccggaa tatggtctgg ataatggtca
     9001 ataacagcaa taaggttgag gtggcccggt atggcgattt ttcatcggtg atcactatcg
     9061 attcattgcc ctccccgagg tatattgcct ttaagggtga cctggcatac gtttcgagct
     9121 ggaataacaa gatatttgca atagatacaa gatcttacaa aatagtggat gaatttccta
     9181 caggtacggg ccccgatgaa atggtgattg cgggggatta tctttttgcc gttaatagcg
     9241 ggggattggg caccacaaat acaatttcat tcataaatat taaacaaaag gaggtcaatg
     9301 gtgaaattac agttggtgat cgtccatgcg gtattactga agataaatac gggaaactgt
     9361 gggttttatg cacaggaaac ggttggaacg gatggcccag tcctgagacc gatacacctg
     9421 gtaaattggt ctgcattgat ccctctacac gccagataat tggagaattc ccgttttctg
     9481 atcctgcctt gcacccggat aacctgatta tcaacggtga gcgatcggtg ttgtattacg
     9541 cctatcctga tggaatttac agcgtttctg tcgataatcc ggccctggcg gttgaaccgc
     9601 ttgttccttc tacagtgatg tattacggat tggggtatga tgttgcagaa gatattattt
     9661 acgctgcaga tccgctggat tacgctcaga atgggatcat ataccgttat agtgccatgg
     9721 atggtttgct gattgatttt tttactgccg gcatggttcc gaatggtttc tggtttaatt
     9781 gattgaaccg ggttctttat tttttgttta ttattgtatg atgaaaacga aaattgattg
     9841 tacctggaaa gagaacatgg cctttgaggc agatgtcaat ggttttaagc taatgctgga
     9901 tgcagatcct gccgtcgggg gagaaaacca aggccctcgg cccaagcccc tgacactggt
     9961 atcactggcc ggttgtaccg ggatggatgt tatctccatc ctgaaaaaaa tgagggtaga
    10021 acccgattac ttcaacgttg ccgttgaagg cgagcttacg gaagaacatc cgaagtatta
    10081 tcacaaaatc caccttgtat atgaaatcaa gggccatgac atcgacccgg agaaagtgga
    10141 aaaagccgta agcttatctc aggaacgtta ttgcggcgta agtgctcttt taaaggctgg
    10201 agcggagatc acgcatgaaa tccggatact gagctgatca cgtctgtaag ttcgtggagg
    10261 atcatggcgg tggctcccca gatgatttga tcttcaataa aatagcaggg tgccgttatt
    10321 cttgtccctg ggaaaatttc gatttgtttc tccgtcagat tctcggagtc tgagatcgca
    10381 ttaaatgata tttccagaat tcgatccact tcctgcggat cagggcaaaa ttccgggcga
    10441 agggtgctat accctacgac aggtgacacc aggaaattgc tgggcggtat atacagatcg
    10501 gttaatcttc ccagaaccct tactacgctt cggtcaaccc caatctcttc ttctgattct
    10561 ctcagggcag tagttacgat atctgaatcc gcttcctcat atgcaccgcc tggaaaggat
    10621 atctggccgc tgtgcacacc ggggtactca ttcctcctga taaacacaat atgaggttga
    10681 tccatgatgg gataaaacaa tacgagaaca ccagcttttt tagcatttcc gggtatttta
    10741 acctgcccgc cttctactat tctcttcatg ctcgccatct tcaattgtga agaaagtccg
    10801 ggtaagggtt ttttaaattt atcttcaaga atctttataa acaattcaaa tctttgcatt
    10861 acgttttctt attatctgat ttatgcaaaa ttcccaaatt tttagtattt tcgtcagggt
    10921 ttttgctcta atgaggtgaa tttgtaaatc cgtttcctaa ttatggttaa agaaaaactt
    10981 aaaccgggcc aggaccagaa ggaactggcg gataatgaaa aggagttgat tctgtttaat
    11041 gacgagatca atacgtttga attcgttatc gataccctca tcgaagtttg cggacacgat
    11101 cctttccagg cggagcaatg tgctttgaca gcccatttca acggcaaatg cggcgttaaa
    11161 tcgggcacct ttcatgagct taaacccgtc tacgacgaaa tgatcagccg gcagttaact
    11221 gtttcaattt cctgaatatg cactgggtat taatcgctac attcattctc atcgggctgg
    11281 tattcctggt gctcgagatc ctggtcatcc ctggcgtggg tgtggccgga atcatcggat
    11341 ttgtcctgat tgctgtcggg gtatggcagt cgtatgctgt ttacggactg gccgccggtc
    11401 atgctgttct tggaggaaca ctggtgctta ccgtactcac attattttta tcgcttcggg
    11461 gaaaaacctg gaaacggata tcgctgtcga catccatcga cagtaaagtc aacgtaatcg
    11521 atcaaaagaa tataaaaccc ggggattcgg gcaagacggt ttcgcgactg gccccgatgg
    11581 gtaaagccat tatcaatgaa gaattttatg aagtgacaac caatggcgat tttatcgatc
    11641 agcaaacgga aattacggtc gtaaaaatcg accacaataa aataattgtt aaacgtaaag
    11701 aatagaaatc atgggtgatt cacaagtttt tatcatagtg gctatcggat taggcgccat
    11761 ctttgccatc tggttgattt tttatttcat ccccgtcggg atatggttta aagcgctggt
    11821 atccggagtc cggatatcct tgcttcaact ggtgatcatg cggtggcgga aagtaccggt
    11881 gacaatcatt gtcaacagcc tgatcgcggc tacgaaagcc ggcctgcaac ttaaccggaa
    11941 ctcgctggaa gcccacttcc tggccggcgg caatgtaacc cgggttgtca attccctgat
    12001 atctgccgat aaagccaata tgaaactgga ttttcaaacg gcaacggcta ttgacctggc
    12061 cggccgcgat gtccttgaag cagtgaaaat gtctgtgaac ccaaaggtaa tcgatacccc
    12121 ttctgttacg gcggttgcca aagacggtat ccagctcatt tgcaaagcca gggtgactgt
    12181 ccgtgccaac atcaaacaac tcgtcggtgg agccggcgaa gaaaccgtcc tggcaagggt
    12241 aggtgagggg atcgtctcat ccatcggttc agcccattcc cataaatcgg tactcgaaaa
    12301 tcccgacagc atatccaaac tggttctcga caaagggctc gattccggta ccgcatttga
    12361 aattctttca atcgacatag ctgatatcga tgtcggtaaa aatatcgggg ctttccttca
    12421 aatggaccag gcaaatgccg ataagaatat cgcccaggcc cgcgccgagg aaagacgtgc
    12481 catggccgtc gctacagaac aggaaatgaa agcaaaagca gaagaagccc gggctaacct
    12541 gatccaggcc gaagccgaaa tccccaaagc cattgcggaa gctttcaggc agggaaacct
    12601 tggaattatg gattatcaac gttatcagaa tatcaaagcc gataccaaaa tgagggagtc
    12661 aatctccgaa cccccatctt ctaaatccaa gaagggcatt aaagaccagg aaaaagaaag
    12721 ctaaccagat ttaccgggga tgatctctga aaccgatctt caaaagatat cggcaaaata
    12781 cgaaggacta ttggcttcag cacaaggatt gctgaaaaaa gatgatcgtc agctccttga
    12841 tgttgccttt gaatttgccc gtaatgtata tggcgataca cgccatgtct ccggtgagtt
    12901 gatgatctgc cattgccttt ccattgcccg gatagcagcg gaggagatga gtcttgggat
    12961 caatgcactc attgctgccc tttttcatga tatccaggcc atacaacctg ccggtctcga
    13021 agaaataaag aagagattcg gtgatccggt tgcggctatg gttaaaagct attcccgcat
    13081 cagcgaactt ccgaccggga aaatttcact gcagtcggat agcttcaggc aactgttcct
    13141 ggccgttgtc gacgatatcc gggttatcct tctcaaactg gcgcaccggc tcagggatat
    13201 gcgaatgatc gataaattgc ccaataatta ccaggagcgc tatatcaacg aagtcaacca
    13261 tctgtatagt ccgattgctc atcggctggg cctgtacagg ataaaaaaag aacttgagga
    13321 tctttcgatg aaattctcgc atcctggcgt ttatcaggac atagccagga agatcaggga
    13381 gacggaagcc aagcggaatg catttatcaa tgaattcatc tctccgattc aacgtgaatt
    13441 attcaagcag ggatttagct tcgatattaa aggtaggccg aaatcgatcc catccatctg
    13501 ggaaaaaatg aaaaagcagg acgtggagtt cgaacaggtc ttcgacctgt ttgctatccg
    13561 gattatcctg aattcaacgg ctgagcatga aaaatccgat tgctggaaag cttattcgat
    13621 cgtaaccaat atctattctc ccaatccaaa acggttacgc gactggatat caacacccaa
    13681 agcttccgga tacgagtcgc ttcacaccac ggtcaaaggc cctaacgatc gctgggtgga
    13741 agtgcagatc cgatcggccc ggatggatga gatcgcagaa aaaggacaag cagcccattg
    13801 gcgttacaaa ggattcggtt cgaaagaaga tacggaacgg tggatgctcc aggtacgtga
    13861 tatcattgaa cacccggagc aaattgcttt cgacgacacg gtaaccgctg tatctgaaag
    13921 taaaccggga aaagtgttcg tttttacccc caacggagac ctgaaagagc tcgatgccgg
    13981 ctcgaccgtt cttgatttcg cttttgaggt ccatacaagt gttggttatt cctgtaccgg
    14041 ggcgaaagtc aataacaagt tagtccccat taaacagacg ctcgagaacg gcgataaagt
    14101 ggagatcata acatccaaga tccagaagcc taagatggac tggcttaatt ttgtcgttac
    14161 atcaaaggca aaaaataaaa taaaacgggc actgaaagag gaacagttcc ttgaagctga
    14221 gaagggaaat gaaatcctca ggagaaaatt caaaaactgg aaaattgcat ttatcgatga
    14281 aaatatcgat aaactgatcc ggaaatataa actgtcatcg tcaattgacc tctatgggct
    14341 gatctataac gagaagatcg accttgtcga aatgaaaagg tttcttgccg gagagcccga
    14401 gaaaccggaa gaaagcaaaa taccggcaaa ggatattaaa ccggtccaga cttccagggg
    14461 aacggcccag gaatccaacg aagtcctttt cctggatacc aatctcaata aagtcaatta
    14521 taagcttgcc cgttgctgca atcctcagcc aggcgacccg gtattcggct ttgttactat
    14581 cagccggggt ataacgctac atacccaatc ttgtccgaat gcacgccagt tgctgacccg
    14641 ctttggatac cggaagatcg acgtccagtg gaaaacctcc ccggataaaa acctcgtccg
    14701 ggcagtcata aacgtaacgg gtaccgaccg tatcggtatt atgaatgaga ttacgcagat
    14761 tatctccaat gatctaaaga ccaatatgat atcagtcaag atcgatgtca ggaagaatat
    14821 ctttaatggt attattaaat tattggtaaa aaacacctct tctttaaacg accttatcgg
    14881 gagaatttca aaggtctccg gtgtcagcaa ggtcgttaaa atagattaat cttaaatatt
    14941 tgttaaatat tttcatcttt ttctttaact ccttaggttt gcacaaatct aaaatttaaa
    15001 atatgaagaa gatgagatta tttacctttt tgatcattgc tctatctatt gcccttgtat
    15061 cctgtgataa ggataatgat ggaaataaca acggaatggg aggcacgatg actttaaaac
    15121 atgatggttc gaactggtct gccagcctgg ctgttgctgc ggctaacaca aatggcgtat
    15181 tatcggtcac cggtagcgat aatagcgcaa accagtgcaa cgtgaccctc tacaacattc
    15241 ccggaccggg aacctaccag ctgggcggca gcatgtcgaa tccaaatacg ggtcgctgga
    15301 cccagggact tggccagaat gatacctatt caactatggt gggtcaaggt acgggaacat
    15361 gcacaattac cgaactgacc gcatcaaaag tctccggaac ctttgagttt actgcaaaaa
    15421 atactgccgg caatatggtc acgatctccg aaggaagctt ctctgccgat ctttagaatc
    15481 tctgtggatc cgttcctatt cgtccaattc gtgtcatctg tgttccatta aatgatggat
    15541 gatcacgaaa gcaataagaa cggatttatt tttaacattt tttacgaaat atttcgtaaa
    15601 taagattgac ttcagaacag aattgatcta tctttgtctt acgaaaagat tcgtacagaa
    15661 atgaaaaaca caccgaaaaa gttcaaaccc acagaaacgg aactcgaaat tctttccatt
    15721 ctttggaata aaggaccggc aacggtcagg gatgtgaatg acgagatgag caggagcaag
    15781 cccaccgggt ataccaccac tttgaaactg atgcaaataa tggcagagaa agggctggtg
    15841 gagagggaaa tggatggcag gtcgcatatt tatcatgcgt tagccaggca ggaagatgcc
    15901 caggtgagta tgcttgaccg gctcctcgaa acagctttcg gcggttcagc ttctaaattg
    15961 gttatgcaaa cccttggcaa tcataaaacc accgaatcgg agctgaaaga gatcaaactg
    16021 ctgattaaaa agttggagga ggagaaatga tgacagctat cggttccatt ctttctgacc
    16081 acgctgtaac aacctggtca gaaagaatgg aaccgatagc tgtcatcatt tctcctcctc
    16141 ataaaatacg gtattaccct ctcacaagta atcgatgctc ttgcaaagtc a
//
LOCUS       DAOPNH010000036        16026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3576_length_16026_cov_5.689688, whole genome shotgun sequence.
ACCESSION   DAOPNH010000036 DAOPNH010000000
VERSION     DAOPNH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3576_length_16026_cov_5.689688"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..967
                     /locus_tag="PKZ74_03310"
     CDS             317..967
                     /locus_tag="PKZ74_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPM91813.1"
                     /translation="MEFLSLSTVFNIFSSPEDLIRIGGLVLLLVIIYLETGFFLGLVL
                     PGGDYLIFTAGLLAGTHFLDVPVYVLVPAMMGAAVLGDYTGYAKGRWLGPKLFDKEDN
                     RFFKRSYLARSRAFYRKFGTWAFIISRFLPVVRTMMPMLAGASGLKLRKFSFYNILGG
                     VIWIGTLAPLGYFLGKKYPNLMDYSIWFLLGFIIIASTPVITSFLKKRKLKVIKHP"
     gene            1049..1963
                     /locus_tag="PKZ74_03315"
     CDS             1049..1963
                     /locus_tag="PKZ74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPM91814.1"
                     /translation="MEINIWFWIGFNAFVVLMLALDLGVFHRKAHEVGFKEAMTWSGI
                     WISLALMFNLGIYYYFGKVKAVEFLTGYIIEKSLSVDNIFVFVLIFSSFAVPAKYQHR
                     VLFWGIIGALIMRAVFIFAGVALINKFHWIIYVFGVFLLYTGIKIAINKGTKINVENN
                     RLLNLFRRYFPVTKEYHGSKFITRIEGKTWATPLMMVLILVETTDLIFAVDSIPAILA
                     ITNDPFIVYTSNVFAILGLRSLYFALAGILKYFHYLHYGLAAILVFVGIKMLITDFYK
                     FDPFVSLGIIGFILLTSIVMSIWKPAKE"
     gene            2064..2456
                     /locus_tag="PKZ74_03320"
     CDS             2064..2456
                     /locus_tag="PKZ74_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPM91815.1"
                     /translation="MIIGIETYPDTYRQHYNLLHLLDRMILARGIRPGDYFKDPWNVF
                     DFIVVAVCFIPAIGTHYVAVLRLARILSVFRVISIFPKLQLLVGALLIASLRWAISFS
                     SWRLLKYKTSETSDVKAMIAILIKRSTI"
     gene            complement(2512..6879)
                     /locus_tag="PKZ74_03325"
     CDS             complement(2512..6879)
                     /locus_tag="PKZ74_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM91816.1"
                     /translation="MNKIHSQDECAITFTHGQGFTTTLSSVTANNDDSHTIVLVVAHD
                     GTTGQGYSRMMNYSVEADPGTYSNIIVQPLTGNFSYLNVEPGPSLPGAPFQGFRINRT
                     NNIGPGMPASFSITFTLTGILQDQQVMVKTFSNQFVSLIQDDDFEEVYDCLFPEIIPY
                     YEPPAGGKTSDLIGAELTSLHNTYINNGSYISNDIFQIVDTLVIIDVFALPGQFDSLL
                     AILTSPAMGMIPENSNPDAGVITGLFPIINLLFINELPDKVSYARPVYPALGNAGLVT
                     SQGDTAMITYLARSGFDLSGNGIKIGVISDSYNTQQGNQAGDDVLRGDLPGPANPVYA
                     APVDVVKEFPYGTRSDEGRAMLQIVHDIAPGAELAFRTGFLGAADFAAGIHELHQNGC
                     NIIVDDITYISEPFFRDGIVARAVDSVKSLGVTYISAAGNFGTKSYESAFYPVPAPDG
                     ITGMAHNFAGGGNGTDIYQSISLTPGNYTVVLQWDDGTPENFTATDFDIYLTKENGAT
                     LFGFNRVNTGGAPIEVLPFTVLGDNVLSNLMIVRASGSGPAFLKYIIFRGDIQINEYA
                     TASPTLVGQANAAGSIAVGAVLYSNTPEYGVNPPTIASFSSRGGTPVNGVNRNKPDIS
                     APNGVNTSVNLGGVNIDGDAFPNFFGTSAAAPHAAGLAALLMEASQKYRSAALSPGEM
                     KSILQSTALDMGTPGYDAASGAGFIHAGAALGTIANPAPLITGIYYDTALVPGKDTLE
                     VTVTGRLLTAGAAIYINGQPAAGEQVLLGDTAIRSVILPFDERYPMIQVYNPPMAGTN
                     GTDGGLSNPLYFSTKETIVMAIDDKFKKYGEVLPDFTAQFFVEGINGTVPLDSAALTQ
                     PEKARITGIQMATVANNMSNTGLWAIEADPGDPLNPESGLDISDPLDLAILERFDIDI
                     ENGLLQIEKMDLVIYPNDTAFVYGDSINGFSFGFHLGQVHDSAYSIFIEPSDSLAILS
                     SLRSNYATALVNGTALVNATALVNEYGEPLLDATALVNKSMMISNATFSRFATALVNG
                     TFIGAPSFLTATALVNVVSFSGSSALAPATALVNGDVVVNGFSTGGATALVNAGQLIN
                     AFAGATALVNTTTVNGNNNSDAIVILGEDDILILSGDSTGGVDLRGISLITGNTVGQH
                     YIVPGSFLSNNFNITYGLGELTILPDTVTVTADMKVIKAGDPLPSFTATFSGFHDGDD
                     QSVVDTLYFTLSPAYNGAAGTYQIQPHATSANYIFVPVTAPLYVNPYGSGAKQITLSI
                     PCIEVLNPPDTSGFTYLARFTYQNTNTTDVYIPYGPDNMFSGSGSWNGINQPVLFKAG
                     GGSFTVPFNGVLLSYTVKSYKNTTKTTSMARVSSQNGLCLKSEEVKEEEAGDNLQESI
                     KAYPNPTNGKVWIRLDGESVSREQVSLVDLYGKVYPVDVSVVSKELIELDLGNYKAGV
                     YLVRIRTDEETETIRVIRK"
     gene            complement(7217..9361)
                     /locus_tag="PKZ74_03330"
     CDS             complement(7217..9361)
                     /locus_tag="PKZ74_03330"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM91817.1"
                     /translation="MNRKKLLTGVFCVTCLLINAQWSWLNPLPQGHNILDAWFFSQDS
                     GIMVGSGGTIMNTHDGGNSWSIQSISNYEESCYLYDLSFADRNCGCLIDTWKNVWKTS
                     DGGSTWVRIYKDEDDNDIRSVAFRDNTHGFIAGSEGLLLVSDDGGISWQQNGNMPDEY
                     FTKISFITPDSGYILSWQGSIFNTQDGGMTWNLQSSGVWTPLEDLLFINSQLGYVYGQ
                     NGVLLKTTNGGDEWNLCFPQDSSLSIYTLCGTGADTLIMIAEKDMGWYMDLRFERSFD
                     GGQSWLEIPYDNDDPWPYVSLYLGDGTCFFAGGHGLMIKTTDCGNSWTYLSTLITPNL
                     ISDIDFPTNEVGYASMLNYYYPTTDISVLKTIDGGDSWFVLDSLFESQVFYSVEFINE
                     NVGFIGGLNIYMTLDGGQSWTKRYSSDWERVITSIDFPNTALGIAVGQQGLILRTTNM
                     GFTWNAVESGTNFDLLSVDMPDPDTGYAAGGGIFLKTTDGGENWNPVPVSFTDLAEIC
                     FLNTRMGYVTCTEDIMKTTDGGLTWQNITPYNVTEYFRHVSFFDSDTGYAGGGRHFTS
                     SVLMKTTNGGINWAKESVPTGYPIECIYVNQANKVFASSHDLYFFGKSNDGMTGISPS
                     LPGLNTMGATCYPNPFCSSTTITYYLSSRAKVKLSVTDVKGDPVCELFEGYQDAGTHE
                     VIFRAGHLKPGVYFYQLLINNENFTGKFVKIK"
     gene            9572..12025
                     /locus_tag="PKZ74_03335"
     CDS             9572..12025
                     /locus_tag="PKZ74_03335"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM91818.1"
                     /translation="MNNSFFYILLAVSFISLNTYSQSSCCTYSLTGQDPGPGEAGETF
                     GIGLGDIDNDGDLDAVIIDAYDDMEVYKNNGAGIFTYHQTYGSGNSWFGAYLVDVDGD
                     TDLDIVVSAFYSGSGCEVWENDGSGNFTFAQGGIASSIAMHQITILDLNGDDAPDIFA
                     PAYSGSGGQVWFNDGTGTFVNTNQSLQAGSATQAAMADFDGDGDPDAYVATTNGSPDK
                     VFLNNGSGFFTPTAQELGNAFSSGAAASDVDGDGDMDVVVANWQVPSQVWLNDGNGTF
                     SPGPQIDNDNYAKAVEISDIDYDCDDDVILGSYGSNGLQVWTNDGLGNFTLCFENTNE
                     IYAHGIAVGDMNGDLMPDVWVGNFSSTSGDYIFLKSTPEFVYDTIQICGGDSVYIGCA
                     WRYSDGDFLQAVNCDTLCWYHVFNTPINVSVTLDNTVLSAIPGYDAYQWFDCNTMLPV
                     SGAISHIFNPEITGTYAVAISHSGCADTSACTFVQTPLVDFAGNPTYGMPPLTVNFTD
                     LSTDMVDTWEWDFGDGNTSGQQNPTHVYTEGGQYSVRLKITGPGGSDSLVKQNYIEVL
                     FNPPTAEFSGTPTTGFAPLEVQFTDLSVDSVNSWLWFFGDGGVAFEQNPSHTYETAGS
                     YTVTLQISGPGGNGEMEKTDYINVYSTDIQADFTGTPTTGQAPLTVDFSDLSVGDIDS
                     WAWDFGDGGTASVQNPTHEYTTTGSFTVSLTVNGLAGSSTETKTDYILIPVGMADNRN
                     EAFVIYPNPVKDLLNIRFPCAADRKIVLKTIEGKHVFEKMTSSAFERIDISGYDPGIY
                     ALIIETKGETINAKVVKKD"
     gene            complement(12012..14108)
                     /locus_tag="PKZ74_03340"
     CDS             complement(12012..14108)
                     /locus_tag="PKZ74_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPM91819.1"
                     /translation="MKNRRLLLKRLVPIMYLIILIQVVFSQPTNVDSLRRIVSDLPDN
                     ATKAGQIIQVAKSYLIKQQPDSALFYCNIAYDLSVRIKYLEGEADALYLKSIIYKGNE
                     DLKMALPLAEKCADLFIILNDSHRIAKGYYNLALAYKESENNDLAMVYSQKSLAISRQ
                     IDDSTLVLGNYNCIGSIFSESKTKGDSAAFYYIKALEISKKKGNSAHQAIILNNLGMI
                     FNSEKNYELAKDYIRQSLELSLQLNDLKQIANSKNYLGIIAASEAEYSLAEELYMESL
                     EICKKIDYLRGISDANLNAGDLFYKMKKYREALEYFDNALSGYRQLEFERGIILALMN
                     KSAVYSELGKTSKALEMQDSCLQIMESISDERLKLLIFQNIADNYYKQGNYKNAYETV
                     ISSKALSEKIYDIEKGKAIHSLLVKYEKEQDQSRILNLEKENLRKTSQRNGYLFGGLA
                     IVVVALFTILYLRQRARHERVVSQQKIIQLEEEKKLMAARLLVEGQEEERKRIATELH
                     DGLGVLLSATRMQFSTIMDKSPENKELIERATKMLEQASGDVRKISHNMMPGLLTKLG
                     FYEAVEDLFEKISDTGDIRVMCNITGDQERLPGNREIMLYRIVQELVNNTLKHAGASV
                     MSIRIQILPDALDITYSDDGRGFDYEKALESGSLGLKSILSRVDFLNGKISVDTQAGK
                     GTEFHLVIPVTILNPS"
     gene            complement(14143..14592)
                     /locus_tag="PKZ74_03345"
     CDS             complement(14143..14592)
                     /locus_tag="PKZ74_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91820.1"
                     /translation="MGSILHKPEQTLAFRFVENESKLYVVSNATVELEQGETIRLRES
                     SPVVMPGGEFGTVIHFDILTNSTSNNTFTAQLNVHVLVRQNMNYTGSPTTYEQKIADI
                     RAQIEYILNTYSPNKAYTIQADVVETTGQVGGQTTSTIDSVGQIIII"
     gene            complement(14683..14997)
                     /locus_tag="PKZ74_03350"
     CDS             complement(14683..14997)
                     /locus_tag="PKZ74_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91821.1"
                     /translation="MKSEIKYVKEIRIIKEKDEEGLMAIRARGVASVRRIVAPKLVPK
                     DYTEAPGDGIYELDFTLGCNHEEFMDIDLEVEVVFRIRSLPAWVKGVKINAAENSDIE
                     LV"
     gene            complement(15350..>16026)
                     /locus_tag="PKZ74_03355"
     CDS             complement(15350..>16026)
                     /locus_tag="PKZ74_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM91822.1"
                     /translation="IRIKFVKNNPVKMIKILVADDHQLLIDGIKTALANIQDIVVAGE
                     AYNGQEVIDQLDAGLSVDVLLLDINMPEMDGLQCTKLVSKQYPKVKILILTQYDEKRL
                     VKQLVKHGASGYLLKDSGRDILEKAIRAVVRGEKYFCERLAIRLISQELDEEKGNPLF
                     SKFSEREIEILRLIAEEYSTQQIADKLFLSYHTIESHRSRMISKAGVRNSAGLVRWAN
                     ENGYLL"
BASE COUNT         4116 a         3874 c         3623 g         4413 t
ORIGIN      
        1 aaagatacaa cattatttta atattacgtc atattatgcc taacttaaca ctagtattga
       61 taaaaaataa taccagttgc cgcataagcg accttagctg gcgcggtgcc cggacaagga
      121 acggtccggg caatcgaacg aagtgagata gcgccaggtt cagtcgcgat gcggcaacga
      181 aaaaaaatcc gaatcttacc atcccgactt tttcagttgg acagcttgtc ccggatgcag
      241 cgagggattg gacagctggc ctgatctgaa aaacttttct ccttttcaat tgttattgtg
      301 tattcatatc ataaccatgg aatttctcag tctcagtacc gtattcaata tattttccag
      361 cccggaagat ctgatccgta tcggcgggtt ggtgctgtta ctggtcatta tttacctcga
      421 aaccggcttc tttctcgggc tggtcctccc cggtggcgat tacctgatct ttaccgcagg
      481 gctgctggcc ggtacccatt tcctcgacgt tcccgtctat gtactcgtcc cggcgatgat
      541 gggcgcggca gtcctgggcg attatacagg ctacgccaag ggccggtggc tgggcccgaa
      601 actgtttgac aaggaagaca accggttttt caaacgctct tacctggccc gaagccgggc
      661 cttttaccgc aaattcggca catgggcctt tattatcagc cggttcctgc cggtcgtcag
      721 gacaatgatg cccatgctgg ccggcgcttc cggtttgaag ctcaggaaat tttcatttta
      781 taacatcctg ggaggtgtca tttggatagg tactctggct ccgttgggct atttccttgg
      841 taaaaagtat ccaaacctta tggattactc gatctggttt ttgctcggat tcatcatcat
      901 cgccagcaca cctgtcatca catcattcct gaaaaaacgc aagctgaaag ttattaaaca
      961 tccttaaaaa gggtcttttt tgatgtttta aacaattatc ctgtagaact gttttagcat
     1021 taccttttat cttcataata accaaactat ggaaatcaat atctggttct ggatcggatt
     1081 caatgccttt gtcgtcctga tgctggcact cgaccttggc gtttttcacc gaaaagccca
     1141 tgaagtgggc tttaaagaag ctatgacctg gagtggtatc tggatcagcc tggctttgat
     1201 gttcaacctc gggatctatt actattttgg gaaggtcaaa gcggtggagt tcctgactgg
     1261 ttacattatt gaaaagtctc tcagtgtcga caatatattt gtttttgttt taatattcag
     1321 ctcgttcgca gtccctgcaa aataccagca ccgggtcctg ttctggggta tcatcggcgc
     1381 cctgataatg cgcgctgttt ttatcttcgc cggggtggcc ctgatcaaca agtttcactg
     1441 gatcatctac gttttcgggg tgtttctcct ttatacggga attaagatcg cgattaacaa
     1501 agggaccaag atcaacgttg aaaacaaccg ccttctgaac ctgttccgcc ggtattttcc
     1561 cgttacgaaa gaatatcacg gcagtaaatt cattacccgt atcgaaggta agacatgggc
     1621 aacgcccctg atgatggtac tgatactggt tgaaacaacc gacctgatct tcgctgtcga
     1681 ctccataccg gctatcctgg ccatcacaaa cgatccgttc attgtttaca cttccaatgt
     1741 ctttgccatt ctggggctcc gctcactcta ttttgccctt gcaggcatcc tgaaatattt
     1801 tcattacctg cattacggtc tggcagctat ccttgtgttt gtcgggatta agatgcttat
     1861 caccgacttt tataaattcg atccctttgt ttcccttgga attatcgggt tcatcctgct
     1921 gaccagcatt gtgatgtcaa tatggaaacc ggcaaaagaa tagtttaacc tcctgaatga
     1981 tgaatataac caaaccactg agtgttttca ccgaatatgc ctacttcagg tatttcatcc
     2041 cgggagtgat catttagtcg gccgtgatca tcggtattga gacttaccct gatacatacc
     2101 ggcaacacta taatctcctg catcttctgg atagaatgat cctggccagg ggcattcgac
     2161 ccggggacta ttttaaagac ccatggaatg tgttcgactt catcgtcgtg gcagtgtgct
     2221 ttatcccggc tatcggcact cattatgttg ctgtattgcg attagcccgt atattaagtg
     2281 tttttcgcgt tatatccatc tttcctaaac ttcaactcct ggtgggtgcc ctgctgatag
     2341 catcccttcg atgggctata tcgttctcct cctggcgttt attgaaatat aagaccagtg
     2401 agacgtcaga tgtaaaagcc atgatcgcga tcttgataaa aagatcgacg atctgaaggc
     2461 gaatcggtca atacttaatg ataatcttta atagtcgcga tcccggactc attacttcct
     2521 gatgaccctt atcgtttcgg tttcctcatc tgttctgatc ctcaccaggt aaactcccgc
     2581 cttgtaattg ccgagatcca gttctatcaa ctccttcgat accactgaaa catccacggg
     2641 ataaaccttt ccgtataaat caaccaggct gacctgctcc cggctcaccg attcaccatc
     2701 caggcggatc catacttttc cattggtggg attaggatag gctttgatgg attcttgcaa
     2761 attatccccg gcttcctctt ctttaacttc ttccgattta agacacaggc cgttctgtga
     2821 actgactctt gccattgagg ttgtctttgt agtgttctta taacttttaa cggtataaga
     2881 cagcaataca ccgttgaaag gcacagtgaa cgatccgcca ccggctttga acaacacagg
     2941 ctgattgatc ccgttccagg atcccgatcc ggaaaacata ttatccggac catatggaat
     3001 gtaaacatcc gttgtattag tgttctgata agtaaaccgg gccaggtaag taaatccgct
     3061 cgtatcgggg ggattcagca cttcgatgca gggaatgctt aaagtgatct gctttgctcc
     3121 cgaaccgtaa ggatttacat atagcggagc cgtgacgggc acaaatatat aattggccga
     3181 ggtggcatgc ggctgtatct ggtatgtgcc tgccgcccca ttgtaagccg gactcagggt
     3241 aaaatacagt gtgtcgacaa cagactgatc atcgccatca tggaatccac tgaatgtagc
     3301 tgtaaatgaa ggcagagggt ctccggcttt gatcactttc atatcggcgg taaccgtaac
     3361 ggtatccggt aaaatcgtta attcgcccaa cccgtaagta atgttgaagt tattcgagag
     3421 gaaggatcca ggtacgatat aatgctgccc gaccgtgtta ccggtgatca ggcttattcc
     3481 ccgcaaatca acacctccgg tagaatcgcc ggacaggatc aggatatcgt cttcgccaag
     3541 tatgacgatg gcatcactgt tattgttgcc gttgactgtg gttgtattga ccagggctgt
     3601 ggcgccggca aaggcattta tcagctggcc ggcattgacc agtgccgtgg cgccgcctgt
     3661 actgaatccg ttcaccacga catcgccatt taccagcgct gtggcaggtg cgagggctga
     3721 actgcctgaa aagctgacca cattgaccag cgcagtggct gtcaaaaagg aaggtgctcc
     3781 gataaaggtc ccgttgacca gtgctgttgc aaaccggctg aaggtagcat tggatatcat
     3841 catgctttta ttgaccagcg ccgtggcatc caggagcggt tcaccgtatt cgttgaccag
     3901 cgctgtagca ttgaccagcg ctgtaccgtt aaccagcgcc gtagcataat tggatctcag
     3961 cgacgacaaa atggccaggc tgtcggatgg ctcaatgaaa atggaatagg cagaatcgtg
     4021 cacctgaccc agatgaaacc cgaaactgaa cccgttgatc gaatcgccat atacaaaagc
     4081 ggtatcgttc gggtatatga ccaggtccat tttttcgatc tggagcagcc cgttttcaat
     4141 gtcgatgtcg aatctttcga ggatggccag atcgagcggg tcagaaatat caagcccgct
     4201 ttcgggattc agcggatcac ccggatcggc ttcaatagcc caaaggccgg tgttactcat
     4261 attgttcgcc actgttgcca tctgaatacc cgttattctt gccttctccg gctgggtcaa
     4321 cgcagcagag tcgaggggca cggtcccatt gatcccctcg acaaagaact gtgccgtaaa
     4381 atcgggtaat acctccccgt attttttaaa tttatcgtca atagccatga caatagtctc
     4441 ttttgtcgaa aaatacaagg gattcgacaa accgccatct gttccgtttg tcccagccat
     4501 cggcgggtta taaacctgga tcatcggata ccgttcgtcg aaagggagaa ttaccgaacg
     4561 gatggccgta tcacccagca gaacctgctc gccggctgcc ggctgcccgt tgatataaat
     4621 ggccgcgcct gcggtcagca acctgccggt taccgttacc tccagggtgt cttttccggg
     4681 tacaagagct gtatcgtaat aaatccccgt gataagaggg gcaggattgg ctatcgtccc
     4741 cagggcagca ccggcatgga tgaaaccggc cccggaagct gcatcgtatc cgggagtgcc
     4801 catatccaga gcggtagact gcaggatgga tttcatctcg ccgggcgaca atgcggcaga
     4861 cctgtatttc tgcgaggctt ccatcagaag ggcggccagg ccggctgcat gtggcgccgc
     4921 agccgaagtg ccgaagaaat tggggaaagc gtcgccgtcg atgttcaccc cgcccaggtt
     4981 caccgaagta ttcacgccgt tcggtgcgct gatatcgggt ttattgcggt tcaccccgtt
     5041 caccggcgtc ccgccgcggg atgaaaagga agcgatggtc ggcggattga cgccatattc
     5101 aggggtgttg ctataaagaa cggctcctac ggctatcgat ccggcagcat tggcctgacc
     5161 gaccagcgtc ggcgatgcgg ttgcatattc gttgatctga atgtcgccgc ggaaaatgat
     5221 atatttcaga aacgcaggcc ccgaaccgga agcgcggacg atcatcaggt tgctgaggac
     5281 attatcgccc agaacggtga acgggaggac ttcaataggt gcgccaccgg tattgacccg
     5341 gttaaatcca aacagggtgg ccccgttctc tttggtcagg tatatgtcaa aatcggtggc
     5401 ggtaaaattt tccggagtac catcgtccca ttgcagaaca acagtataat ttcccggcgt
     5461 cagggagatg ctctggtaaa tatcggtgcc gttgccacca ccggcgaaat tgtgggccat
     5521 gccggttatg ccgtcgggag ccggcaccgg ataaaacgcg ctctcataag atttagtgcc
     5581 gaaatttccg gcagcggata tgtaagtcac tccaagcgat tttacgctgt cgacagcccg
     5641 ggccacgatg ccatcacgaa aaaatggctc gctgatatag gtgatatcat cgacgatgat
     5701 attgcatccg ttttgatgaa gttcatgaat gcccgcggca aaatcggcag cgccaaggaa
     5761 accggttcga aacgcgagtt cagcgcccgg cgctatatcg tgcacgatct ggagcatggc
     5821 gcgcccttca tccgacctcg tcccgtaagg aaattcctta acaacgtcga caggtgccgc
     5881 ataaaccgga ttggcaggcc cggggagatc gcctctcaga acatcatcgc cggcctggtt
     5941 cccctgttgc gtgttgtaac tgtcggaaat cacgccaatc ttgatcccgt tgccgctcag
     6001 gtcaaatccc gaccgggcca ggtaagtgat catcgccgta tcgccctgag aagtgaccaa
     6061 gccggcgttg cccaatgcgg gataaactgg gcgggcgtag ctgactttat caggtaattc
     6121 attgataaaa agcaggttaa tgataggaaa aagaccggtt atcacgcccg catccggatt
     6181 gctgttttca gggatcatcc ccatggccgg tgaggtaagt atggccagga gagaatcgaa
     6241 ttgccccggc agggcaaata catcgataat gaccaacgta tcgacgatct ggaaaatatc
     6301 gttggaaata taggagccgt tattgatata tgtattgtga aggctggtga gctctgcgcc
     6361 gataaggtca ctggtcttgc cccctgcagg cggttcataa tacggaatga tctccgggaa
     6421 caaacagtca taaacctctt caaaatcatc atcttgtata agactgacga actggttgct
     6481 gaaagttttc accatcacct gctgatcctg gagaatcccg gtcagggtaa aagtgattga
     6541 gaaagaggcg ggcattccgg gcccgatatt gttggtccgg ttgatcctga acccctggaa
     6601 aggcgctccg gggagcgacg gaccgggttc aacattcagg taactgaaat tgcctgtgag
     6661 cggctgaacg atgatattgc tgtacgttcc cggatcggcc tccaccgaat aattcatcat
     6721 ccgcgaatat ccttgtccgg tcgtaccgtc gtgggcaacg accagcacga tcgtatgact
     6781 gtcatcattg ttggcggtta ccgacgataa agttgtggta aatccctggc catgcgtgaa
     6841 agtgatcgca cattcatcct gcgaatggat cttattcaag aagagcatgt taaagagcaa
     6901 ggcacaggtc agggccacta gaactttgga tctattgcgt ttggttatca tataaagcgg
     6961 ttctggaata tttttattta gaaagtgtct aaacaaagat aaggcgggag ttgtctttac
     7021 aatattaatt aacaatcaat taacaaaata gaccgcgtgg taagtgattg ggtaaagctc
     7081 gctattggtc agcttctaaa aagtgtttcc acttcagact ctggcaggtt tacattaaaa
     7141 atgaattggc agaaatcctg gcgacctgga tgaactgaaa attgaacata gaaattggaa
     7201 attataaata gatgattcat ttaattttta caaatttccc ggtaaagttc tcattattaa
     7261 ttaaaagctg ataaaaataa actcccggtt tcaaatggcc tgccctgaag attacctcgt
     7321 gtgtacctgc atcctggtaa ccttcaaata attcacatac cggatcccct ttgacatctg
     7381 taaccgataa ttttaccttc gccctgcttg ataaatagta agttatcgtt gtcgaactac
     7441 aaaaaggatt aggataacac gtggctccca tcgtatttaa accaggcaat gatggggata
     7501 ttcctgtcat cccatcattc gattttccaa aaaaatatag atcatggctt gaagcgaaca
     7561 ccttattcgc ctgattaaca taaatacatt cgattggata acctgtagga acactttctt
     7621 tggcccagtt aatcccgcca ttcgttgttt tcataagcac gctgctggta aaatgtctgc
     7681 ctcctcctgc atacccggtg tcgctgtcaa aaaagctaac atgccggaaa tactctgtga
     7741 cattataggg ggtaatgttt tgccatgtca atcccccgtc tgtagttttc atgatatcct
     7801 ccgtacaggt cacataaccc attctcgtat tcagaaaaca aatttctgct aaatcggtaa
     7861 aggaaacggg cacaggattc cagttttctc cgccatcagt ggtttttaga aatattcctc
     7921 ctccggcagc atagccggta tcaggatcag gcatatcgac agataagaga tcaaaattgg
     7981 ttccggactc aacggcattc caggtgaagc ccatgtttgt cgttcgcaat attagtcctt
     8041 gctggccaac ggcaattcca agggctgtat tcgggaagtc gatagaggtg atcacccttt
     8101 cccaatccga ggaatagcgt tttgtccagc tttgaccgcc gtcaagagtc atataaatat
     8161 taaggcctcc tataaatcct acgttctcat taataaattc gacagaataa aaaacctggc
     8221 tctcgaacaa ggaatcgagc acgaaccatg aatctcctcc atcgatggtt ttcagaacac
     8281 tgatatccgt ggtgggatag taataattga gcatggaagc atatccaacc tcatttgtcg
     8341 ggaaatcaat atcactgata agatttggag taatcaatgt tgaaagatag gtccaggaat
     8401 ttccgcaatc agttgttttg atcatcaaac catggcctcc ggcaaaaaag caggtaccat
     8461 cgccaaggta aagggataca taaggccatg gatcatcatt atcataagga atttcaagcc
     8521 agctttgccc tccgtcaaat gatctttcaa agcgcagatc catataccat cccatatcct
     8581 tttcggcaat catgatcagc gtgtcggcgc ctgtaccaca taatgtgtat attgatagcg
     8641 acgaatcctg cggaaagcaa aggttccatt catcgccgcc atttgttgtt tttagtaaaa
     8701 ctccattttg accatataca taacctaact gagaattgat aaaaagcagg tcctccaggg
     8761 gagtccaaac acctgaggat tgcaggttcc atgtcattcc tccgtcttgg gtattaaaaa
     8821 tcgagccctg ccagctcaaa atatatccgc tatcaggagt aataaagctg atctttgtaa
     8881 aatattcatc cggcatatta ccgttctgct gccagctaat tcctccatca tcacttacca
     8941 gtaacagccc ttcggatcct gcgataaaac catgtgtatt atccctgaaa gccacacttc
     9001 tgatatcatt atcatcttca tccttatata tacggaccca ggtactacca ccatcagaag
     9061 tcttccagac atttttccag gtatcaatta aacatccaca atttcggtct gcgaaagaaa
     9121 gatcatacaa atagcaggat tcttcataat tactgatgga ctgaatagac catgaatttc
     9181 ctccatcatg ggtattcatg attgttccac cgcttcccac cataatcccg gagtcctgag
     9241 agaaaaacca ggcatctaat atattatggc cctgaggcaa aggattaagc cacgaccatt
     9301 gtgcatttat gagcaggcaa gttacacaga aaacgccagt taggagtttc tttctgttca
     9361 tcaggacgct gacaaattaa gcataaagtt ataaacttta attacttttt gtcataatta
     9421 agtgaatata atcccagaat tgctattcat tccttcaggg actctcaggc ttcttataac
     9481 tacaaaggct aacgatgtaa ttgatatttt ttctattttc atcgggatta tcagcaatga
     9541 tgccctatta ataaataaaa tgtatatgat catgaataac tcattctttt acattctgtt
     9601 agccgtatct ttcattagcc tgaacacgta ctcgcagagc tcctgctgca cctattctct
     9661 gaccggccag gatccgggac ccggtgaagc aggcgaaacg ttcggtatcg ggctgggcga
     9721 tatcgataac gacggcgacc tggatgccgt gatcatcgat gcttacgacg acatggaggt
     9781 ttataagaac aacggagccg gtatctttac gtatcatcaa acctatggct ccggtaactc
     9841 ctggtttggc gcttacctgg tggatgtcga cggcgatacg gacctcgata tcgtggtttc
     9901 ggctttctat tccggctcgg gatgcgaggt ctgggagaac gacggcagcg gcaactttac
     9961 cttcgcccag ggtggaatag cctcatccat cgccatgcac cagatcacta tcctcgacct
    10021 gaacggcgat gacgcacccg acatctttgc tccggcttac agcgggagcg gcgggcaggt
    10081 ctggttcaac gacggcaccg gcacttttgt caatacgaac caatcgttgc aggccggctc
    10141 ggctacccag gccgcgatgg cggatttcga cggcgatggc gaccccgacg cctatgtcgc
    10201 caccaccaac ggctcgcccg ataaagtgtt cttaaacaac ggctcgggat tttttacacc
    10261 tactgctcag gaactgggaa acgccttttc atccggcgct gctgcatcgg atgtcgacgg
    10321 cgacggggat atggacgttg tggtggccaa ctggcaggtg ccctcgcagg tatggcttaa
    10381 cgatggaaat ggcacctttt cacccggccc ccagatagat aatgataact atgcaaaagc
    10441 ggtggagatc agtgatatcg attatgattg cgatgatgat gtcatactgg gctcctatgg
    10501 ctcgaacggg ttacaggtat ggacaaacga cgggctgggg aattttaccc tgtgttttga
    10561 aaatacgaac gaaatatacg ctcatggcat tgccgtcggg gatatgaacg gtgacctgat
    10621 gccggatgtc tgggtcggca acttcagctc aacttcaggc gattatattt tcctgaaatc
    10681 gaccccggaa tttgtttatg ataccatcca gatctgcggt ggcgacagtg tctacatcgg
    10741 ctgtgcctgg cgatattccg acggcgactt cttgcaggcc gtcaattgcg ataccctttg
    10801 ctggtatcat gtgttcaaca ccccgatcaa tgtgtctgta acgcttgata atactgtcct
    10861 ttcggctata ccgggctatg atgcttacca atggtttgat tgcaacacca tgttaccggt
    10921 ttcaggagct atcagccata tttttaatcc tgagatcacc gggacctatg ccgtagcgat
    10981 ctcgcactcg ggttgcgccg acacctcggc ctgcaccttt gttcaaacgc ctcttgttga
    11041 ttttgcaggt aacccgactt acggaatgcc tcccctgacg gttaacttta ccgatctctc
    11101 gacagatatg gtcgatacct gggagtggga tttcggtgat ggcaatactt ccggccagca
    11161 aaatccgacc catgtctaca ccgaaggggg acaatattcg gtccggttga aaattacagg
    11221 tcccggtggc agtgacagcc tggtgaaaca aaattacatc gaggtgttat tcaatccccc
    11281 aactgcggaa ttctcaggaa ctccgaccac cggttttgcc ccgctggaag tgcagtttac
    11341 cgatctttcg gtggatagtg tgaatagctg gctatggttc ttcggcgacg gcggggttgc
    11401 ttttgaacag aatccgtcac acacctacga aaccgccggt tcgtatacgg tcaccctgca
    11461 aatttccggt ccgggcggca atggggaaat ggagaagacg gactacataa atgtctattc
    11521 aacggatatc caggctgatt ttaccggtac cccgacaacc gggcaggcac cgctgacagt
    11581 tgatttttcg gacctttcgg ttggcgatat tgattcctgg gcctgggatt tcggcgatgg
    11641 cggcacggcc agtgtgcaga acccgactca tgaatataca accaccggca gtttcacggt
    11701 ttctctcact gtaaatggcc ttgcagggag cagtacagaa accaaaacgg attatatcct
    11761 gattccggtc ggcatggctg ataacaggaa tgaagctttt gtgatctatc ccaatccggt
    11821 aaaggacttg cttaacatcc ggtttccctg tgctgccgat cgaaagatcg tccttaaaac
    11881 catcgaaggg aagcatgtat tcgaaaaaat gacctccagt gcatttgaga gaatcgatat
    11941 aagcggatat gatccgggaa tctatgcgct gatcattgaa acgaagggag aaaccatcaa
    12001 tgcgaaggtg gtcaagaagg attgaggatt gttaccggga taacaaggtg aaattcagta
    12061 cctttgcctg cttgtgtatc gactgatatt ttcccgttca ggaaatcgac cctcgacagg
    12121 atgcttttca gtcccaaaga tcccgattcc agcgcctttt cataatcgaa gccccggcca
    12181 tcgtcagaat aggtgatatc cagcgcatcg ggaaggattt ggatccggat gctcataaca
    12241 gaagcgccgg catgtttgag ggtattgtta accagttcct ggacgatccg gtaaagcatg
    12301 atctcccggt tgccgggcaa acgttcctga tctccggtaa tattacacat caccctgata
    12361 tcgcctgtat cgctgatctt ctcaaaaagg tcttccacgg cttcatagaa accgagtttt
    12421 gttaacaagc ccggcatcat gttgtgcgag atcttcctga cgtcgccgct ggcctgctcg
    12481 agcattttcg tcgccctttc gatcagttcc ttgttttcag ggcttttatc catgattgtc
    12541 gaaaactgca tccgggtggc cgacagcaac actcccaaac cgtcgtgcag ctccgtggca
    12601 atgcgtttgc gctcttcctc ctgcccctcc accagtaacc tggccgccat cagctttttc
    12661 tcctcctcca gttgaatgat tttctgctgc gacacgactc tttcatgcct tgccctctgg
    12721 cggaggtaca ggatcgtaaa caaagccaca accacaatgg ccagtccgcc aaaaagatac
    12781 ccgttgcgct ggctggtttt gcgaaggttc tccttttcca ggttcaggat ccgggattga
    12841 tcctgctctt tttcatattt caccagtaaa ctgtggatag ctttcccttt ctcaatatca
    12901 taaatttttt cagataacgc cttgctgcta ataaccgtct cataggcatt tttgtaattc
    12961 ccttgtttat aataattatc ggcgatattt tgaaagatca gcagtttcag ccgttcatca
    13021 ctaatgcttt ccataatctg caggcaggaa tcctgcattt caagtgcctt acttgttttc
    13081 cctaattctg aataaacagc cgatttattc atcaaagcca atatgattcc tctctcaaat
    13141 tccaattgcc ggtaacctga taaggcgttg tcgaaatatt cgagcgcttc acggtacttc
    13201 ttcattttat aaaataaatc tccggcattc aaattcgcat cggaaattcc acgcaaataa
    13261 tctatttttt tacaaatttc aagcgattcc atataaagct cttctgccag tgaatattcc
    13321 gcttcactgg cagcaatgat tccaaggtaa tttttactgt tggctatttg cttcaggtca
    13381 tttaattgca agcttaattc aaggctttga cggatataat cttttgccag ttcataattt
    13441 ttctcagaat tgaagatcat tccaaggtta ttcaatatga tagcctgatg cgcagaattc
    13501 cctttctttt tgctgatttc aagagcttta atataataaa aagctgctga atcccccttt
    13561 gttttgcttt ctgaaaaaat actgccgata caattataat ttcccaggac tagagtagaa
    13621 tcgtcaattt gccggcttat tgccaggctt ttttgtgaat agaccatggc caggtcattg
    13681 ttttcagatt ccttataagc aagagccagg ttataatacc ctttggcaat gcggtgactg
    13741 tcgttgagaa taataaatag atctgcacat ttctcggcaa gcggcaaagc cattttcagg
    13801 tcctcatttc ctttgtaaat aatgctttta agataaagtg catccgcttc accttccagg
    13861 tactttatcc tgacggaaag atcatacgct atattacaat agaaaagcgc agaatcgggc
    13921 tgttgtttta ttaagtaact cttggcaacc tgtataattt gaccggcctt ggttgcatta
    13981 tccggaaggt cgctgacaat gcggcgcaag ctgtcaacat tagtaggttg tgagaaaacg
    14041 acctggatca aaatgattag gtacatgata ggaaccagcc gttttaaaag caagcgtctg
    14101 tttttcatca gtcaggagtt ttatcaggtt ccaatgattc ccttaaatga ttatgatctg
    14161 gcctactgaa tcaatagttg aggtcgtttg cccgccaact tgcccggtcg tctccaccac
    14221 atccgcctga atggtataag ccttgtttgg agagtacgta tttaaaatat attcaatctg
    14281 agcccggata tctgcaattt tctgttcata tgtcgtcggt gaacctgtat agttcatgtt
    14341 ttgcctgacc aggacatgaa cgtttaattg ggcggtaaag gtgttattgc tggtactgtt
    14401 ggtcaggata tcaaaatgga taactgttcc aaattcgcct cccggcatta caaccgggct
    14461 tgattcacgt aaccttattg tttcgccttg ctcaagctcg actgtggcat ttgagacaac
    14521 atatagtttc gactcgtttt caacaaatct gaaagccagg gtctgttcgg gtttgtggag
    14581 gatacttccc atatcattgt tgtttaaggt ttagagtccg gtcataaaga tgatgaaaaa
    14641 atacacaatt tttatctcta aagatactat ttgtgaattt aatcatacca attcaatatc
    14701 tgaattttcg gccgcattaa ttttaacgcc ctttacccat gccgggaggc tcctgatccg
    14761 gaaaactact tctacctcca ggtcgatatc cataaattct tcatggttac aacctaatgt
    14821 gaaatcaagt tcatagattc catctccggg cgcttcggta taatctttcg gcaccaactt
    14881 aggagccaca atacgcctga ctgaagcaac ccctcgggcc cggatagcca tgagtccttc
    14941 ctcatctttt tccttaatga tccggatctc ttttacatat ttgatttcac ttttcatctt
    15001 acaatgttat ccattataaa atgaagaaga ttagctgaaa acattgaaaa aaaaaattag
    15061 ctgtttccag ctaatattat tctaaaagca ctgcatacct ttgatctatt aagttcttat
    15121 aaaaatcctg atttggttag aggttaagat cggtggtcaa ccgcaggtcc aggtagtcat
    15181 aattctgaag attatcatag cagaaaatga cctgtaagga agtgtaacat tccttatccc
    15241 cacccaaaag aagcgggaat aattattccg aaatcatccg ccacattccc ggcgaaaatt
    15301 aatccggatt aaccttacca tcaggaaccc cgatccagtc ggggtttttt tataacagat
    15361 aaccgttctc attagcccac cggaccagtc cggcagagtt cctgacgccg gctttgctga
    15421 tcatccgtga ccgatggctt tcaatggtat ggtaactaag aaataattta tcggcaattt
    15481 gttgtgtgga atactcctcc gcaatcagcc ggagaatctc aatctctctt tctgaaaatt
    15541 tagaaaataa cgggttgcct ttctcttcat ccagttcctg gctgatcaat cgtattgcca
    15601 gacgctcaca aaaatatttt tcacccctca caaccgcccg gatggccttc tccagaatat
    15661 cccggcccga atcttttaaa agataacccg aagcgccatg cttgaccaat tgttttacca
    15721 gccgcttttc atcatactgg gtaaggatca ggattttaac tttcggatac tgctttgaaa
    15781 ccagttttgt gcattgtaat ccatccattt ccggcatatt gatatccagc agaagaacgt
    15841 caactgagag ccctgcatct agttgatcaa taacctcttg accgttgtat gcttcaccgg
    15901 caaccacaat gtcctgtata tttgccaggg ccgttttaat cccgtcgata agcagttggt
    15961 ggtcatcggc aaccagtatt ttgatcattt tgacaggatt gtttttaaca aatttaatgc
    16021 gaatta
//
LOCUS       DAOPNH010000037        15982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3592_length_15982_cov_6.260815, whole genome shotgun sequence.
ACCESSION   DAOPNH010000037 DAOPNH010000000
VERSION     DAOPNH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_15982_cov_6.260815"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..585)
                     /locus_tag="PKZ74_03360"
     CDS             complement(85..585)
                     /locus_tag="PKZ74_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91823.1"
                     /translation="MNDEFKKDLDFIQDIVDLRGFIINKTIIVETGIDTIIIYYFFKD
                     NPTNAWEFHDDVIKTLNNNQKINLLKKVLLHFGENIKPNTKKRLFDLLNKIFDLRNKV
                     AHYSWSGVWHDGVYLKKEKLKDEIIIGDNEIRSFIKNCREANALLNIILVVTGYHDQI
                     IKSYSK"
     gene            complement(739..1500)
                     /locus_tag="PKZ74_03365"
     CDS             complement(739..1500)
                     /locus_tag="PKZ74_03365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM91824.1"
                     /translation="MKETIQFDKVADIYDFYVNVNFDIPFFIKETDGYKGEILELMCG
                     TGRVSIPLLDSGRHMICVDYSKGMLNLFERKIKNKNYPVNLVQMDVTNLKLDKKFGLI
                     ILPFHSISEIISTDLQIKALQSISSHLEKDGVFILTLQNPKTRLILADGITRVMGRSS
                     IDESRHLIISYMNQYNESDKIVTGLQFYEIYDSMNILIEKRYLEINFKPVADSELRSM
                     IKAAGLEIVEMYGDYSYGRFDEEKSNFMIYKMTKN"
     gene            1875..2231
                     /locus_tag="PKZ74_03370"
     CDS             1875..2231
                     /locus_tag="PKZ74_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91825.1"
                     /translation="MGKEKNIQDITTEKSPVKFTREEALRDYRVANLSRQLSLIGRRE
                     VLGGKAKFGIFGDGKEIPQLAMAREFRAGDWRSGYYRDQTFMLATGMMTAEEFFAQLS
                     LAIPTWPTTRKRPAGS"
     gene            2228..4315
                     /locus_tag="PKZ74_03375"
     CDS             2228..4315
                     /locus_tag="PKZ74_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPM91826.1"
                     /translation="MNNHFATRSLDENGDWKDLARQKNSSADISPTAGQMPRLLGLAL
                     ASKIFRHTEGLEHMAAFSDHGNEVAFGTIGDASTSEGHFFETINAAGVLKVPMAISIW
                     DDGWGISVAKKFQTTKESISEILRGFEMDKNGNGYLIYKAKGWDYAGLCRVYHEGIAR
                     CRAEHVPVIFHVEECTQPQGHSTSGSHERYKSAERLKWEEEFDGIRKFREWIIAEKLA
                     TAKELDDVEKQAAEEAREARRSAWNHFLEPLKKQRDEFLRMADISTCNCAKTERINAI
                     KDELRMVGEPLWKDIMSSGKKILRLICDSCSNPQNSLKKNVMAWRDQAMKQNEINYSS
                     HLYSESRHNALDVEEVKAVFDKEAPLVPGREILRDNFDHILGHYPETLVFGEDVGGIG
                     GVNQTLEGMQAKYGELRVADTGIREATIIGQGIGLAMRGLRPIAEIQYFDYLPYGLQL
                     ISDDLATLQYRTRGGQKAPMIISTRGHRLEGIWHSGSALSMVINSIRGVHVLVPRNMT
                     QAAGFYNTIIRSDEPALIIEPLNAYRIREPRPSNIGEFTVPLGIPEIIREGADVTIVT
                     YGACVRIALEAAGQLKDFGIDVELIDVQSLLPFDIRHSIVESLKKTNKVLFFDEDVPG
                     GATAYMMQKVLEEQHGYYFLDAQPKTLTAKAHRPAYSTDGDYFSNPNAEDVFDSVYNM
                     MHDINPERYPKIF"
     gene            4316..4885
                     /locus_tag="PKZ74_03380"
     CDS             4316..4885
                     /locus_tag="PKZ74_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91827.1"
                     /translation="MHDIEPYYSWRHLYIASDDHRSPFYNKEYSEFEFTHAVYNYLIH
                     PQWDEMGSATLYLKVLYANYDTGFAIIEFIGEWNDALYNDIMYLKRNIADVMIASGIT
                     KFILIGENVFNFHPLDNDYYEEWFDDIEDGWIVALNFREHVVREFKKAHLDYYLAFGG
                     SFDTFNWRGFEPNQLFRVIDAQVMKRLNP"
     gene            4882..5151
                     /locus_tag="PKZ74_03385"
     CDS             4882..5151
                     /locus_tag="PKZ74_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPM91828.1"
                     /translation="MRKGYGIRVTGKVQNVGFRYYTVKTAAEFNICGFVTNKPDGSVY
                     IEAEGEEDMLEGFLSWCRRGPQWARVEGFEVQEQPVMGFEGFKVR"
     gene            complement(5204..5896)
                     /gene="mnmD"
                     /locus_tag="PKZ74_03390"
     CDS             complement(5204..5896)
                     /gene="mnmD"
                     /locus_tag="PKZ74_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPM91829.1"
                     /translation="MENDTKSPEVEIIRTADGSSSLFVPEMGEHYHSVNGAITESIHI
                     FINAGLKYVMNDQNTVRILEVGFGTGLNTFLTWHEARAAKINVHYTAIEKYPLKQEVY
                     HQLNYPELMGYPFGEECFMKIHELQWDAPHEVMEHFILHKINTDIKDFHPGKNRFDLV
                     YFDAFGPDFQPDMWTTDLFKKMATALRPGGVLVTYSTKGTVKRNLKEAGFRIEKLPGP
                     IGKREILRALVI"
     gene            complement(5896..7173)
                     /locus_tag="PKZ74_03395"
     CDS             complement(5896..7173)
                     /locus_tag="PKZ74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91830.1"
                     /translation="MMRKSLVAIILLGVVLFSCKKEQDTLPPVISFIAPGVNTIINIP
                     DTLDVKVQVNDDRIIHFVVISIVNEDKIPLTEAKSFAPFLAEFTAVHSFVIDSKTIAS
                     GVYSIQVLAFDGFNTKTGFLDIEINEIPRSLEGFMAVTAPLSFQSEIFRLGQDFSIDS
                     QMSFPQGCQFTAVHPVWERFLFAGGDPSVLTAINPFTFVTEWELPATPPRPQFTAIEA
                     GKDILFATANGDAGIVDIDGNVTLRTFPYNNMKILCLAADENYMYAAQQSTGGTNNEL
                     TLFYRETGAVKSRKLVAERISGIVALDDQALVFFSPGNRTEIFHYNPESFILNKLNEI
                     EGEILVSAEKVSDDQIIICTDAAIRLFRPGINRLDMLLEQPYRFCRYDALGNRLFLAK
                     DYEMEVRNFTTLDLTEEISFNDMIVDFRILYNK"
     gene            7261..7971
                     /locus_tag="PKZ74_03400"
     CDS             7261..7971
                     /locus_tag="PKZ74_03400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM91831.1"
                     /translation="MLKDIAIENVLFLDIETAPAFPEYSKAEERLKKLWDKKSEQLKR
                     EPDDTPESLFPRAGIYAEFGKVICISAGLFKGREFRIKSFYGDDEYQLLVQFCKMLNS
                     HYGGEKKMLCAHNGKEFDFPFLARRILIHGIPLPAILDVAGRKPWEVQFLDTMELWKF
                     GDYKHYTSLELLAAIFGIPTPKDDISGSDIHRVYWEDHDLERIRTYCQKDVLTVARLF
                     LKYQQQPVIEDDDVILVE"
     gene            8118..9206
                     /locus_tag="PKZ74_03405"
     CDS             8118..9206
                     /locus_tag="PKZ74_03405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPM91832.1"
                     /translation="MMPITKPTLIVNKEQCLANISAMAEKARMTETQFRPHFKTHNSA
                     HIGNWFRKMGIHKITVSSVSMAKFFSAYGWRDITVAFPVNLLELGQISELAAELTLNI
                     IGDTSAILPALENGLTYRAGFFIEIDTGHHRTGVFWKDLAEIDRMLEFLSGSNRLQFK
                     GFLTHAGHTYSADSHEEIIDIYKDSVRKMAYLKDFYKEAWPELIISIGDTPGCSLAED
                     FAGVDEVRPGNFIFYDLMQYSLGSCDLEQIAVVMACPVVSKSIQRNEITIYGGSVHFS
                     KDFLYKSNGEHLYGYVVKLDDKGWSKPLQSSYLASLSQEHGIIRAPKDFVEEVSIGDV
                     LGIIPVHSCLTANLMKGFVTLEGEAIGY"
     gene            9361..10896
                     /gene="dnaB"
                     /locus_tag="PKZ74_03410"
     CDS             9361..10896
                     /gene="dnaB"
                     /locus_tag="PKZ74_03410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPM91833.1"
                     /translation="MEENTRYNPSDKSKGFARKGFERRNAPQENVFEHGKVQPQAVDL
                     EEAVLGAIMLEKDALTAVIDILKPDVFYKDQHKVIYKAIISLFSKSEPVDILTVTSEL
                     KKSGDLEVAGGAYYITQLTNRVASSANVEYHAHIIIQKYIQRELIRISSEIIKDSFED
                     SMDVFDMLDKAEQQLFTVSDSNLRRNTLDMNTLVKEALHYIEAAKNQQAELRGVPSGF
                     TEIDRITSGWQRSDLIILASRPGMGKTAFALSMARNMAVDFHKPVAIFSLEMSSVQLV
                     TRLISAESMLPAEKLRKGDLADYEWEQLNSKISRLIDAPIFIDDTPALSIFELRAKCR
                     RLKAQHNIEMVFVDYLQLMVGAYDRQGNREQEISNISRSLKSLAKELDIPVLALSQLS
                     RAVETRGGSKKPILSDLRESGAIEQDADLVLFIYRPEYYQIDQLDDGTPTNGLAVISI
                     AKHRNGALGERNLKFTAKYARFSDYDSVEPDQSFIDGHLPERQKTYQSKMNDMEEEEG
                     MPF"
     gene            complement(10901..11563)
                     /locus_tag="PKZ74_03415"
     CDS             complement(10901..11563)
                     /locus_tag="PKZ74_03415"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPM91834.1"
                     /translation="MAQVFTPAHYDAERAEMVDQQIGARGIRDEKVLAAMLKVERHRF
                     VLPEYLDEAYADHPLPIGDGQTISQPYIVAFMTEALDLKATDKVLEIGTGSGYQAAVL
                     AEICDSVFTIEIFPSLARQADNILKSLGYTNIFIKTGDGYQGWPEHAPFDAVIVTCAP
                     EEIPGPLKDQLAEGGKMIIPVGGQYTQELVLAEKKNGKLVKTNVLPVRFVPMIDSTGR
                     KY"
     gene            complement(11637..12827)
                     /gene="sucC"
                     /locus_tag="PKZ74_03420"
     CDS             complement(11637..12827)
                     /gene="sucC"
                     /locus_tag="PKZ74_03420"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPM91835.1"
                     /translation="MNLHEYQGKSILRKYKVAVPLGIVAFTPSEAIKAAEHIQDETGS
                     DKWAVKAQIHAGGRGKGGGVKVAKSLEEVEQFARQIIGMKLVTPQTGPEGKEVRKILI
                     EQNIYYPGPSDVKEFYMSLLLNRAIGRNMIMYSPRGGMDIEKVAEETPDQIFHEEIDP
                     RTGLMPFQARRIATNLGLNDDAFKNMVKFVHALYDAYMQSDAKLFEINPVFKAADDRI
                     FAGDAKVVIDDNALFRHPDIQEMRDYTEEDPIEVEASRFRLSYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGASPANFLDVGGTADAKRVEEAFRIILRDPNVKAILVNIFGGIVRC
                     DRVARGIVDAYKNIGEINVPILVRLQGTNAEEGAKIINESGLRVQSAVRLEDAAELVS
                     NVLS"
     gene            12944..13594
                     /locus_tag="PKZ74_03425"
     CDS             12944..13594
                     /locus_tag="PKZ74_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM91836.1"
                     /translation="MLVARGIHKSFGDLKVLKGIDIEVSEREIVSIVGSSGAGKSTLL
                     HIIGTLDKPDSGSILYDGTDISGWGEERMAGFRNQSIGFVFQFHHLLPEFTALENVCI
                     PAFIAGIPRKEAEQKAKHILDLLNLSDRLEHKPSALSGGEQQRVAVARALVNGPRIVL
                     ADEPSGNLDSASAKELHRLFFRLRDEIGQTFVIVTHNLELASMADRTISMKDGKIE"
     gene            13614..>15982
                     /locus_tag="PKZ74_03430"
     CDS             13614..>15982
                     /locus_tag="PKZ74_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91837.1"
                     /translation="MKNRYITLVTLIIFLLLRAIPAISQTDMEQYSKTIQMADNYFSK
                     GDYINAKASYQIAVKLAPDEQYPKDRLQQSLDMIKVQMYQNSLYTQQIQLADEFFGKK
                     DFDNALKYYQEALTILPGDVYASGKIQEINMTRANNQQTEEDYIKAITAADKLFKEGK
                     LEGSLAEYRRAVELKPSETYPKEKAIEVEALIAKGKKITDEYELALQDAELALSRNDY
                     ETAVQKLEAAIRLMPGDPLPKQKLAEAQNLKAAWDSYSGLINEGDDLYVSKEFEKARQ
                     KYLQALSVKPSEEYPRSMIEKIDAALLQVAEQNITSYEEAIALADQLYSEEKYEQAMV
                     EYKNALRFKPDENYAKQRISDITNALNLRKSQEEAYNQSIAKGDKLYREGQYEQALAE
                     YKKAYDFKPMEQYPKVKIAEIESKIANRVSQQGTYDELIKGADKLFFTDNYFEALGQY
                     KKALEIFPEEKYPQGQIIMINEILGLRERYNASVAKADQLYGEKQYEPALLEYRNATS
                     IMPSETYPLEKIRELENIISRQTALNEKPVTEEINEEEDFQADEGETVVITQQGQTFE
                     IEADSAEIRQQTEMENRYWAAIETGDQKLAGKEYMEAKAAYQEARALKPGEVYAEGKI
                     KEIDLILADIAAKEAAARELAAKEAAEKEAAARELAAKEAAEKEAAEKEAAAKELAAK
                     KAAEKEAAARELAAKEAAEKEAAAKELAAKEAAEKEAAAKELVAKEAAEKEAAAKELT
                     AKEAAEKEAAAKELAAKEAAEKEAAAKELAAKEAAEREAAAKEQINREYL"
BASE COUNT         4281 a         3856 c         4139 g         3706 t
ORIGIN      
        1 cgccttcgcg gggaaaggtt ttgcaacatt tcttcagaat ctgatccgat ttatcagggt
       61 tgggttgtgc cgcaaaaagt tcatttattt gctgtagctt tttataattt gatcatgata
      121 accagtaacg acaagtatta tatttaggag cgcatttgct tctctacaat ttttaataaa
      181 actcctgatc tcattgtcac cgatgataat ctcgtccttg agcttttctt tctttaaata
      241 gacaccgtca tgccaaactc ctgaccacga ataatgagct actttattac gcaaatcaaa
      301 aatcttgttt aataagtcga atagtctttt ctttgtgttt ggtttaatat tctcgccaaa
      361 gtgtaataat actttcttta aaagattaat tttttgattg ttattaagag ttttaatgac
      421 atcatcatga aattcccatg catttgttgg attatcctta aagaaataat atataatgat
      481 tgtgtcaata ccagtctcaa cgattatggt cttattgata ataaagccac gcaaatcaac
      541 tatatcttgg ataaaatcta aatctttttt gaattcatca ttcataatac aagaagttac
      601 ggccaacggt tgacaatatg gccagtaagg gattgcgggc gatttgctat caggttacac
      661 gaaaaattaa agcagacttc ttacttcgca tacccctgaa acccttattg gatataacgt
      721 atgttgtgcg ttcctgcttt aattttttgt cattttataa atcataaaat tacttttctc
      781 ttcatcaaaa cggccatatg aataatctcc atacatttca acaatctcca atccagcagc
      841 tttaatcata ctccttaatt ccgaatcagc tactggttta aaattaatct ccaagtacct
      901 tttctcaatc aggatattca tcgaatcgta aatttcatag aattgtaatc cagtaacaat
      961 tttatcagat tcgttgtatt gattcatata tgagatgatc aaatgtctac tttcatcaat
     1021 cgaagatctc cccattaccc ttgtaattcc atctgctaat attaatcgag ttttaggatt
     1081 ttgaagcgtt aaaataaaaa caccatcttt ttcaagatgt gatgaaattg attgcaatgc
     1141 tttaatctgt agatcagtcg atattatttc actaattgaa tgaaatggca aaataatcaa
     1201 accaaatttt ttatccaatt tcagatttgt cacatccatt tgaaccaaat tgacagggta
     1261 attcttgttt ttgatttttc gttcgaataa atttaacatc ccttttgaat aatctacaca
     1321 tatcatatgc cttccagaat caagcagcgg aattgatact cgtccggttc cgcacattaa
     1381 ttccagaatt tcacctttat aaccatcggt ctctttaata aaaaaaggaa tatcaaaatt
     1441 tacattgaca tagaaatcgt atatatccgc aactttatca aattgtatag tttctttcat
     1501 ctgtcgaaga gtttattagc atgacgcaca attgttgcct aacacccaaa tcctccccat
     1561 taccgcaatc cccccaaccg agcatgctaa ctgtatcgcg tttaacactc caaatataag
     1621 gagtaatttc aaccagtata tagattgaga taagatattt atacatgcta aactcttgac
     1681 ccgcaatctt tagtaatccg tgaatattcg ccgtatccgt gtttattcgt gtgctataat
     1741 gggaacacgg atgatacgga tgagacgaat taacacgaat ggggtttatc agcgaagggc
     1801 ggttggctcc ggtttatgat caaatgcagc tttttcacta cctttgcacg catcattcta
     1861 gctggtcttg accgatggga aaagagaaaa atattcaaga tatcactact gaaaagagtc
     1921 ctgtaaaatt cacccgggaa gaggcgctgc gcgattaccg tgtggccaac ctgagccggc
     1981 aactgagcct gatcgggcgg cgcgaggtgc tgggcggcaa ggccaaattc ggcatcttcg
     2041 gcgacgggaa agagataccg caactggcga tggcccggga gttccgcgcg ggcgactggc
     2101 gctcgggcta ctaccgcgac cagacgttca tgctggctac gggcatgatg acggccgaag
     2161 aattcttcgc gcagctctct ctggcgatac cgacgtggcc tacaacccgc aaacggccgg
     2221 ccgggtcatg aacaaccact ttgccacccg cagcctcgac gaaaacggcg actggaagga
     2281 cctggcccgg cagaaaaact cctcggccga catctccccc acggccggac aaatgccgcg
     2341 gctcctcggg ctggcgctgg cttccaaaat tttccgccat actgaagggc tggagcatat
     2401 ggccgcgttc tcagatcatg gcaacgaggt ggccttcggc actatcggcg atgcgagcac
     2461 ctctgaaggg catttcttcg aaacgatcaa tgcggccggg gttctaaagg taccgatggc
     2521 gatctcgatc tgggacgacg gctggggcat ctccgttgcc aagaaattcc agacgacaaa
     2581 ggagagcatt tcggagattc tgcggggttt tgaaatggac aaaaacggca acggatacct
     2641 gatctacaag gcgaaaggct gggattatgc cggtctgtgc cgggtttacc acgagggcat
     2701 tgcccgctgc cgcgccgagc atgtgccggt cattttccat gtggaagaat gcacccagcc
     2761 gcaggggcac agcacctccg gttcgcacga gcggtacaaa tcggccgaac ggctgaaatg
     2821 ggaagaagaa ttcgacggca taaggaagtt cagggaatgg atcatcgctg aaaagctggc
     2881 gacggccaaa gagctcgatg acgttgagaa gcaggccgca gaagaggcca gggaggcaag
     2941 gaggtcggcc tggaaccact tcctggagcc gctgaagaaa cagcgcgacg aattcctccg
     3001 catggccgat atttccacct gcaactgcgc caaaacagag cggatcaatg cgatcaagga
     3061 tgagctgcgg atggtcggcg agccattgtg gaaagacatc atgtcgtcgg ggaaaaagat
     3121 cctccggctg atctgcgatt cgtgcagcaa tccgcagaac tcgctgaaaa agaatgtgat
     3181 ggcctggagg gaccaggcga tgaagcagaa tgagatcaac tacagctcgc acctgtacag
     3241 cgaatcgcgg cacaacgccc tggatgtcga agaggtgaaa gcggtgttcg ataaagaggc
     3301 gccgctggtg ccgggccggg agatcctgcg cgacaacttc gatcacatcc tgggtcatta
     3361 cccggagacg ctggtattcg gcgaggatgt gggcgggatc ggcggggtga accagacgct
     3421 ggaaggtatg caggcgaaat acggcgagct gagggttgcc gacacgggca tccgtgaggc
     3481 gacgatcatc gggcagggga tcgggctggc gatgcgcggg ctgaggccca ttgcggagat
     3541 ccagtatttc gactacctgc cgtacgggct gcagctcatc agcgacgacc tggccacgct
     3601 gcaataccgg acccgcggcg ggcagaaggc gccgatgatc atttcgacgc ggggacaccg
     3661 gctcgaaggg atctggcact cgggctcggc gctcagcatg gtcatcaact cgatccgcgg
     3721 tgtgcacgtg ctggtgcccc gcaacatgac ccaggccgcc ggcttctaca acaccatcat
     3781 ccgctccgac gagccggcac tgatcatcga accgctgaac gcctaccgga tcagggagcc
     3841 acggccatcg aacatcggcg agttcaccgt tccgctggga atcccggaga tcatccgcga
     3901 aggcgccgat gtgaccatcg taacctacgg cgcctgcgtc aggatcgccc tggaggcggc
     3961 cgggcaactg aaagacttcg gtatcgatgt ggaactgatc gacgtgcagt cgctcctccc
     4021 cttcgacatc cgccactcga tcgtcgaatc gctgaagaag accaacaagg tgctgttctt
     4081 cgatgaggac gtgccgggcg gggcaacggc ctacatgatg cagaaggtgc tggaggagca
     4141 gcacggatat tatttcctcg acgcccagcc gaagacgctc acggccaaag cacaccggcc
     4201 ggcgtacagc accgatggcg actatttcag caatccgaat gcggaagacg tattcgattc
     4261 ggtgtacaat atgatgcacg atatcaaccc ggaacgctac ccgaaaatct tttaaatgca
     4321 cgacatcgaa ccatattatt cctggcgcca cctgtatatc gcttcggatg accaccgttc
     4381 gccgttttac aacaaagaat actcggaatt cgagtttacg cacgccgttt ataattacct
     4441 gatccatccc cagtgggacg agatgggttc ggcaacgctt tacctgaaag tcctgtatgc
     4501 aaattacgac acaggttttg ccatcatcga attcatcggc gagtggaatg atgcgctgta
     4561 caacgacatc atgtacctca agcgcaatat tgccgatgtc atgatcgcca gcggcatcac
     4621 aaaattcatc ctgatcggcg agaacgtatt caatttccat ccgctcgaca acgattatta
     4681 tgaagaatgg ttcgatgata tcgaagacgg ctggatcgtg gcgctgaact tcagggaaca
     4741 tgtggtccgc gaattcaaaa aagcgcacct ggattactac ctggccttcg gagggagttt
     4801 cgacacgttc aactggcgcg gatttgagcc aaaccagttg ttcagggtga tcgacgcaca
     4861 ggtcatgaaa cggctgaatc catgagaaag ggttacggga taagggttac gggaaaggtg
     4921 cagaatgtcg ggttccgcta ttacacggta aaaacggctg ccgaattcaa catctgtggt
     4981 tttgtgacca acaagccgga tggctcggtt tatatcgaag cggaaggaga agaagatatg
     5041 ctggaaggat ttttgagctg gtgccgccgc gggccgcaat gggcgagagt ggagggattc
     5101 gaggtgcagg agcagccggt gatggggttt gaggggttta aggtgagata ggcgaattat
     5161 tcaccacaga ttacacagag ttacgcagag gagcactgag atatcaaatt accaatgccc
     5221 gtagaatctc gcgtttgcca ataggtcccg gcaacttctc aattctaaac cccgcctctt
     5281 ttaaatttct tttcaccgtt cctttcgtgg aatacgtgac cagcaccccg cccggcctca
     5341 atgcagttgc catcttctta aaaagatctg tagtccacat gtccggttga aaatcggggc
     5401 cgaatgcatc gaaataaacc aggtcgaacc ggttcttacc cgggtggaag tccttgatgt
     5461 ccgtatttat tttatgaaga atgaagtgtt ccataacctc gtgcggtgca tcccattgca
     5521 gttcgtgaat cttcataaaa cactcttctc caaaaggata acccattaat tccggataat
     5581 tcagttgatg ataaacctcc tgttttaaag gatacttttc aatggctgtg taatgcacat
     5641 taattttcgc ggcccgggcc tcgtgccagg tcaggaaagt attcagcccg gtgccaaagc
     5701 cgacctccag gatcctgacc gtattctgat cattcatgac atatttcagg ccggcattga
     5761 taaagatatg gatcgattcg gtgatggccc cgtttacgct gtgataatgt tcgcccattt
     5821 ccggaacaaa aagagaggac gaaccgtcgg ctgtccggat gatctccact tccggggatt
     5881 tcgtgtcgtt ttccatcatt tattataaag aatcctgaaa tccacgatca tatcattaaa
     5941 agaaatctcc tccgtcaaat ccagtgtggt gaaattcctc acctccatct catagtcctt
     6001 agccaggaat agccggttac ccagtgcatc gtagcgacaa aaccggtaag gctgctccag
     6061 gagcatgtcg agccggttga tgcctggacg aaagaggcgg atagcggcat cggtacaaat
     6121 aataatctga tcatcagaaa ctttctcggc agacaccagg atctcgccct caatttcatt
     6181 caatttattc aggataaagc tctccgggtt ataatggaag atctcggtcc ggttgccggg
     6241 cgaaaagaaa accagcgcct gatcgtccaa agctacaatt ccggatattc tttcggcaac
     6301 aagtttccgg gacttcaccg caccggtttc cctgtaaaac agggtgagtt cgttgttggt
     6361 cccgccggtc gactgctggg cggcatacat ataattttca tctgccgcaa ggcagagtat
     6421 cttcatgttg ttgtacggaa atgtccggag ggtcacattt ccgtcgatat ccacgatccc
     6481 tgcgtcgccg ttggctgtag cgaacagaat gtctttaccg gcctcgatgg cagtgaactg
     6541 aggcctgggc ggcgtggcgg gaagctccca ttcggtcaca aaggtaaagg ggttaatggc
     6601 tgtcaatacc gaaggatctc ctccggcaaa gaggaaccgt tcccagacgg gatgaactgc
     6661 tgtaaactgg cacccctgag ggaaactcat ttgcgaatcg atgctgaaat cctggcccag
     6721 ccggaagatc tccgattgga agctgagtgg agcggttacg gccatgaaac cctctaatga
     6781 cctggggatt tcattgatct cgatatccag gaagccggtt ttggtattga atccgtcgaa
     6841 agccaaaacc tggattgaat aaacacccga tgcgattgtt ttggaatcga tcacaaacga
     6901 atgaacggct gtaaactccg ccagaaaagg ggcaaaagat ttggcttccg taaggggtat
     6961 cttgtcttca ttcacgatgc ttattaccac aaaatggatg atacggtcgt cgttaacctg
     7021 cactttcaca tcgagagtat cgggaatatt gataatggtg ttcacacccg gggcgataaa
     7081 actgatgacc ggaggcagtg tatcctgttc ctttttacag gaaaataaaa caacaccaag
     7141 caggatgatc gcaactaaag attttctcat catcttacaa aaataatgcg gataacggaa
     7201 aaatgaagct cagggaatgg catcccgtat gtttttataa ttttagcaaa aattgaattt
     7261 atgttgaaag atattgccat agagaacgta ttgttcctgg acattgagac ggctccggct
     7321 tttccggaat acagcaaggc ggaagaaagg ctgaagaagt tatgggacaa gaagagcgag
     7381 cagttgaagc gggaaccgga cgacacgccg gaaagtctat tcccgagagc aggcatttat
     7441 gctgagttcg gtaaggtgat ctgtatttcg gcgggtttgt tcaagggcag ggagtttcgg
     7501 ataaaatctt tttatggcga cgacgagtac cagctactgg tgcagttctg taaaatgctg
     7561 aattcgcatt acggcgggga gaagaagatg ctttgcgccc acaacggcaa ggagtttgat
     7621 tttccgttcc tggcgcggcg catcctgatc cacggaatac cgttgccggc catcctcgat
     7681 gtggcgggac gaaagccctg ggaagtgcaa ttcctggata cgatggagtt atggaagttc
     7741 ggcgactaca agcattacac ctccctggaa ttgctggctg ccattttcgg tatacccaca
     7801 ccaaaggacg atatcagcgg aagcgatatt caccgggttt actgggagga tcatgacctg
     7861 gagcggatca ggacctattg ccagaaggat gtcctgacgg tcgcccggct tttcctgaaa
     7921 taccagcagc aacccgtgat cgaagatgat gatgtgatcc tggtggaata acgggtagcg
     7981 gtctattatg cccaaacccc tacacttcag aatacagaaa aatacataca tttgttttac
     8041 caacggacaa cggacaacgg acaacggaca acggacaatg gacaacggac aatggacaac
     8101 ggacaatcct tacttgaatg atgccgataa caaaaccgac tttaattgtc aataaagagc
     8161 aatgcctggc caacatcagc gcgatggcgg agaaggcccg gatgacggaa acgcaattca
     8221 ggccgcattt caaaacgcac aattcggcgc atattggcaa ctggttccgg aaaatgggca
     8281 ttcacaagat cacggtttca tcggtcagca tggccaaatt tttttccgca tacggctggc
     8341 gcgacatcac ggtagcgttc ccggtcaacc tgctggaact cggccagatc agcgaactgg
     8401 cggctgagtt gaccctgaac attatcggcg acacatcggc tatccttccg gcattggaga
     8461 acgggctcac ataccgggcc gggtttttca ttgaaatcga caccgggcac caccggacgg
     8521 gtgttttctg gaaagacctg gcggagattg accggatgct ggaattttta tcgggctcga
     8581 accggctgca attcaaagga tttttaaccc atgccggcca tacctactcg gccgacagtc
     8641 atgaagagat catcgatatt tacaaagaca gcgtcaggaa gatggcctac ctcaaagatt
     8701 tctacaagga ggcctggcct gaactgatca tttcgatcgg ggatacgccc ggatgcagcc
     8761 tggcggagga ttttgccggc gtcgacgaag tacggccggg caatttcatt ttctacgacc
     8821 tgatgcagta ttcccttgga tcgtgtgacc ttgagcagat tgcagttgtg atggcctgtc
     8881 cggtcgtcag caagagcatt cagcgcaacg agatcacgat ttacggcgga tcggtccatt
     8941 tttccaaaga ctttctctat aaaagcaacg gcgaacatct ttacggttat gtcgtgaaac
     9001 tggatgataa aggatggtca aaacctcttc aaagctcata cctggcgagc ctttcgcagg
     9061 agcacgggat catccgggca ccgaaagatt ttgtcgagga ggtcagtatc ggggatgtgc
     9121 tgggtattat tccggtacat agttgcctga cggccaatct gatgaaagga tttgttacgc
     9181 tggaaggcga agcaatagga tattgaagga ttgtccattg tccgttgtcc attgtccatt
     9241 gtccgttgtc catttttcag ggaattgggc tttttttcac attttttcaa cacgaatttt
     9301 ggtgataaaa tctggtttat gaaaggataa ttcggaggtt gatgatgtat ctttgacctc
     9361 atggaagaga acacccgata caacccgtca gataagtcca aaggcttcgc caggaaagga
     9421 tttgagagga ggaacgctcc gcaggaaaat gtttttgaac acggcaaagt acagccccag
     9481 gcggtagatc tggaagaggc ggtgctgggg gccatcatgc tggaaaaaga tgccctgacg
     9541 gcggtgatcg acatcctcaa gccggatgtg ttttacaaag accagcacaa agtcatctat
     9601 aaagcgatca tcagcctttt ttccaaatcg gaaccggtcg atatccttac cgtgacgagt
     9661 gaactgaaaa agagcggaga cctggaggtt gccggcggcg cttactatat cacgcagctg
     9721 accaaccggg tggcctcgtc ggccaacgtg gagtaccatg cccacatcat catccagaaa
     9781 tatattcagc gggagctgat ccggatctct tccgagatca tcaaagattc ttttgaagat
     9841 tcgatggatg tttttgacat gcttgacaaa gccgagcagc agcttttcac cgtcagcgac
     9901 agcaacctgc gccgtaacac gctggatatg aatacactgg tgaaggaagc gttgcattat
     9961 atcgaagcgg cgaaaaacca gcaggccgaa ttaaggggcg tgccgtcggg atttaccgag
    10021 atcgaccgga tcacatcggg ctggcagcgg tcagacctga tcatcctggc atcgcgcccc
    10081 ggtatgggaa agaccgcttt tgccctctcc atggcacgga atatggcggt cgatttccat
    10141 aagccggtgg ctattttctc ccttgaaatg tcgtcggtac agcttgtcac ccgtctgatc
    10201 tctgccgaat cgatgctgcc ggcggagaag ctccgcaaag gcgacctggc cgattacgag
    10261 tgggaacagc tcaattccaa gatcagccgc ctgatcgacg cgccgatctt catcgacgac
    10321 acccctgccc tctcgatctt tgaactgcgg gccaaatgcc gccgtctgaa agcgcagcac
    10381 aatatcgaaa tggtatttgt cgattacctg caactcatgg ttggcgccta cgaccggcag
    10441 ggcaaccgtg agcaggagat cagcaacatc tcccggtcgc tgaagagcct ggccaaggag
    10501 ctcgacattc ccgtgctggc cctgtcgcag ctcagccgtg ccgttgaaac ccgcggaggc
    10561 tcgaagaagc ctatcctttc cgacctccgt gagtcgggcg ctatcgagca ggacgccgac
    10621 ctggtactct tcatctatcg tccggaatac taccagatcg accagctcga cgacggtacg
    10681 ccgaccaacg ggctggcggt catcagcatt gccaagcaca ggaacggtgc actgggcgag
    10741 agaaacctta aattcaccgc caaatatgcc cgcttctccg attatgattc ggtggagccg
    10801 gatcagagct tcatcgacgg ccacttgccc gagcggcaaa agacttacca gtcgaaaatg
    10861 aatgacatgg aagaggagga gggaatgcct ttctaggtgc ttaatatttc ctgccagtgc
    10921 tgtcgatcat cgggacaaac cggacaggca gcacattggt tttgaccagt ttcccattct
    10981 ttttttctgc caggaccagt tcctgggtat attgaccacc taccgggatg atcattttgc
    11041 caccttcggc gagctggtct ttgagcgggc cgggaatctc ttccggcgcg caggtaacaa
    11101 tgacagcatc aaaaggggca tgctccggcc agccctgata gccatccccg gtcttaataa
    11161 aaatattcgt ataacccaat gatttcagga tgttatcggc ctggcgggcc agcgagggaa
    11221 agatctcgat ggtgaaaacc gaatcgcaaa tctcggcgag tactgccgcc tggtatccgc
    11281 tgccggtgcc gatctcgaga actttatccg tggctttcag atccagcgct tcggtcatga
    11341 aagcgacaat ataaggctgg gagatggtct gtccgtcgcc aattggcagc ggatggtcag
    11401 catacgcttc atcaagatat tcgggcagga caaaccggtg tcgttccacc ttcagcatgg
    11461 ctgccagtac cttttcgtca cggattcccc tggcaccgat ctgctggtcc accatttctg
    11521 ctctttcagc atcgtaatgt gcgggtgtga atacctgtgc catgctagcc gggttaaact
    11581 gcaacaagat ggttaagatg aagaatactg acctccaatc agttaaacgc attatttcag
    11641 gataaaacgt tgcttaccag ttcggcggcg tcttcgagcc gcacagccga ttgcacgcga
    11701 aggcccgatt cattgataat tttcgcccct tcttcggcat tggtgccctg caaccggacc
    11761 aggatcggga cattgatctc tccgatattt ttgtaagcgt cgacgatccc ccgggcgacg
    11821 cggtcgcagc ggacgatacc gccgaagata ttgaccagga tggctttcac attgggatca
    11881 cgaaggatga tccggaaggc ctcctccacc ctcttcgcgt cggctgttcc gcccacatcg
    11941 aggaaattgg ccggtgacgc gcccgaaagc ttgatgatgt ccatcgtggc catggccagc
    12001 ccagcgccat taaccatgca acccacgttc ccgtcgagtt tgacatagct gagcctgaag
    12061 cggctggctt cgacctcgat cggatcttct tccgtatagt cgcgcatctc ctggatatcc
    12121 ggatggcgga acagggcgtt gtcgtcgatc accaccttgg cgtcgcccgc aaaaatacgg
    12181 tcgtcggctg ctttgaagac cgggttgatc tcgaacagct tggcatccga ctgcatatag
    12241 gcatcgtaga gtgcatggac aaatttcacc atgttcttga aggcgtcgtc gttcaggccg
    12301 aggttagtgg caatacggcg ggcctggaac ggcatcagcc cggtgcgcgg gtcgatctct
    12361 tcgtggaaga tctgatcggg ggtttcttcg gccactttct caatatccat tccgccccgg
    12421 ggcgaataca tgatcatatt gcggccgata gcccggttga gcaacaggct catatagaac
    12481 tctttcacat ccgatgggcc gggataatag atattttgtt cgatcaggat tttcctgacc
    12541 tctttacctt ccggtcccgt ctggggcgtt accagtttca tcccgatgat ctgccgggca
    12601 aattgctcga cttcctccag cgatttagcc actttgaccc cgcctccttt gccgcggccg
    12661 ccggcatgga tctgggcttt caccgcccat ttatcggagc ctgtctcatc ctggatatgt
    12721 tcagctgctt taatggcttc ggatggcgta aaagctacga tgccgagcgg tacggccact
    12781 ttatacttcc tgagtataga tttcccctgg tattcgtgta agttcatgtg attcgtttaa
    12841 aaggcttgat atgcaaatgt aacgatttta ggttcaggat taatatccgt aataaaatca
    12901 tcctgatttc gttatatctt tgccgctcat ttcgcaacaa gacatgctgg tagcacgagg
    12961 aattcacaaa tcattcggtg atctgaaagt cctgaaaggg attgatatag aagtatcgga
    13021 aagggagatt gtctcgatcg tcggttcatc gggggcggga aagtcgaccc ttttacatat
    13081 tatcggcacc ctcgacaagc ccgattccgg ctcgatctta tatgacggaa cggatatttc
    13141 gggatggggc gaggagagga tggccggttt caggaatcag tccatcgggt ttgtattcca
    13201 gttccaccat ttgctgccgg agttcacggc tctggagaat gtatgtattc ctgcctttat
    13261 tgccggtatt ccgcggaaag aggcggaaca aaaggcgaag catatcctgg atctgttaaa
    13321 tctgtcggac cggctggagc ataagccttc cgctttatcg ggcggcgaac agcaacgggt
    13381 tgccgtggcg cgcgccctgg tcaacggccc gaggattgtg ctggccgacg aaccctcggg
    13441 aaacctcgac tcggcttcag caaaagagct gcaccgtttg tttttcaggt taagggacga
    13501 aatcgggcaa acgttcgtta tcgtcacgca caacctggaa ctggcctcta tggccgaccg
    13561 tactatctcc atgaaagacg gcaagattga ataaagttaa ccgtgtccga tctatgaaga
    13621 accgatatat aacgctggta acgttgatca tctttttgtt gctgagggct attcctgcca
    13681 tctcacaaac agatatggaa caatattcga agaccatcca gatggccgac aactactttt
    13741 caaaaggcga ttatatcaat gcaaaggctt cttaccagat cgccgtaaag ctggctcctg
    13801 acgaacaata tcccaaagac cggctgcagc aatcgctgga tatgatcaag gtccagatgt
    13861 atcaaaacag cctttatacg cagcagatac agttagcgga tgagttcttc ggcaagaaag
    13921 acttcgacaa tgccttaaag tattaccagg aagcgctgac gatccttccc ggggatgttt
    13981 acgcttcagg gaaaatccag gagatcaaca tgacccgggc aaataaccag caaaccgagg
    14041 aagattatat caaagccatc accgcagcag ataaactttt caaggaaggc aagctggaag
    14101 gttcgctggc ggagtaccgt cgggctgtcg aactgaaacc atcggaaact tatccgaaag
    14161 aaaaagccat tgaagtagaa gcgctgattg ccaaaggcaa aaaaattacg gatgagtatg
    14221 aactggcctt gcaggatgca gagctggccc tgtcaagaaa tgattacgag accgctgttc
    14281 aaaagcttga agcggccatc aggctgatgc cgggagatcc tttgccaaaa cagaagctgg
    14341 ccgaagctca aaacctgaaa gcagcctggg attcctactc cgggctgatc aatgaaggcg
    14401 atgatcttta tgtttccaaa gaatttgaaa aagccaggca aaaatatttg caggctctgt
    14461 ctgtcaagcc atccgaagaa tacccacgga gcatgatcga aaagatcgat gcggctttac
    14521 tgcaagttgc cgaacagaat attaccagct atgaagaagc cattgccctg gccgaccagc
    14581 tgtacagcga agagaagtac gagcaggcta tggttgagta caaaaatgca ttgcggttca
    14641 aacccgatga aaactatgcc aagcagcgga tcagtgacat caccaatgcg ctgaacctga
    14701 gaaaatcaca ggaagaagct tataaccaat cgattgccaa aggcgacaag ctttaccggg
    14761 aggggcagta tgagcaggcc cttgcggaat ataaaaaagc ctatgatttc aaaccgatgg
    14821 aacagtatcc aaaggtgaaa atcgccgaga ttgagtcaaa gatcgctaat cgggtcagtc
    14881 agcagggaac gtacgacgag ctgatcaaag gggcagacaa attattcttt accgataatt
    14941 attttgaagc gctggggcaa tacaaaaagg cgctggaaat tttcccggaa gagaagtatc
    15001 cacagggcca gatcattatg atcaacgaaa ttctgggttt gcgtgaaagg tataatgcct
    15061 ccgttgcaaa agcagatcag ttatacggag agaaacagta tgaacctgct ctcctcgagt
    15121 acaggaatgc gacttcgatc atgccatccg agacatatcc cctggaaaag atcagggaac
    15181 tggaaaatat tatttcacgg caaacggcat tgaatgaaaa gccggtaacg gaagagatca
    15241 atgaagaaga agattttcaa gcggacgagg gtgagacggt tgtgatcacg caacagggac
    15301 agacctttga aatagaagca gattcagcgg aaatccggca gcagacggaa atggaaaacc
    15361 ggtactgggc tgccattgaa acaggagatc agaaactggc aggcaaagaa tacatggagg
    15421 caaaagcagc ctatcaggag gcacgcgcat taaagcccgg tgaagtttat gcggaaggca
    15481 agatcaaaga gatcgacctg atcctggctg atatcgcagc aaaagaagcg gctgcaaggg
    15541 agctggcagc aaaggaggcg gctgaaaagg aagcggctgc aagggaactg gcagcaaaag
    15601 aggcggcgga gaaggaagcg gctgaaaagg aagcggctgc gaaggagctg gcggctaaaa
    15661 aagcggctga aaaggaagcg gctgcaaggg agctggcagc caaagaagcg gcagaaaaag
    15721 aagcggctgc aaaggaactg gcagcaaagg aggcggctga aaaggaagcg gctgcgaagg
    15781 agctggtggc caaggaggca gcggagaagg aagcggcagc gaaagaactg acagccaaag
    15841 aggcggcgga gaaggaagcg gcagcgaaag aactggcagc caaagaagcg gcagaaaaag
    15901 aagcggcagc gaaagaactg gcagcaaagg aagctgccga aagagaagct gcagcgaaag
    15961 aacagatcaa cagggagtat ct
//
LOCUS       DAOPNH010000038        15913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3615_length_15913_cov_5.715223, whole genome shotgun sequence.
ACCESSION   DAOPNH010000038 DAOPNH010000000
VERSION     DAOPNH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3615_length_15913_cov_5.715223"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKZ74_03435"
     CDS             <1..708
                     /locus_tag="PKZ74_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM91838.1"
                     /translation="IYGILLGLILFAGATFQQVGIVYTTAGNAGFITGLYVILVPVFG
                     IFSGQKARVNLWGGALIATVGMYFLSVTEAFSISKGDLFVLTGAVFWAVHVLYTGWVS
                     PKVSAIRLAAVQYAVCALLSLFAALIFEKNTLSGIYDAAIPLLYGGFLSVGVAFTLQI
                     VGQKKAPPAHAAIILSLEAVFAVVGGMLILSETMTDRKWFGCALMLAGMLLAQVKSRQ
                     SAVSSQQSAVSSKYGEN"
     gene            726..1586
                     /locus_tag="PKZ74_03440"
     CDS             726..1586
                     /locus_tag="PKZ74_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPM91839.1"
                     /translation="MKPGTWNPEPGTRNLEPGTWNLEPGTWNLEPGTKPKHMNLKLEV
                     CASSLTSALNAQAGGAHRIELCDNLNEGGTTPSAGTVILAKKLLGIPVFVLIRPRAGD
                     FTYSDVEFESMQEDILFCKRNGLDGIVTGILTGSGNVDIDRMRILVDLARPMQVTFHR
                     AFDLTPDPIKALEEIISLGIERILTSGQAENALAGAKLIKQLNNRTNNNIIIMPGGGV
                     TENNIHLLANQTGTQEVHASLRSPVNSSMCNRNKTVSIKKNPSDDYSWMETDRRRVKK
                     MYELMTKLYE"
     gene            1741..2577
                     /locus_tag="PKZ74_03445"
     CDS             1741..2577
                     /locus_tag="PKZ74_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91840.1"
                     /translation="MKKFYILLIMMLTFSGFAFSQVSSRMDNPPAPVMASPESHVTRA
                     SGCNFLVFMDNLPWNSSAIQTILTANGESWVLQNSASMASTDFSMYDVIIIASDQIPS
                     FYDNFAANFAKFVTFVTNGGSLEVHAATCGWNSPCGYSVLLPGGVFTTEQYDDYNLVV
                     DAAHPIVAGVSSPFSGSYASHGYFSNLVAGTDIITETSSNNKPTTIQYVYGAGIVTAT
                     TCTYEFGYDFGQEVGEMLVNNLNYGCEHAIDAKVPVSDWAIAIGIFMILFAAVLRYRR
                     VF"
     gene            2671..5835
                     /locus_tag="PKZ74_03450"
     CDS             2671..5835
                     /locus_tag="PKZ74_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM91841.1"
                     /translation="MHKSYLLFFSFLFSFICQAQQIPEWENPQVTGVNKVQPHATLIP
                     YPDERTAIENVKSKSPFYLLLNDEWKFNWVEKPFDRPAGFYNPDFDDSGWNNIPVPAN
                     WELLGYGVPIYVNISYEWTRNPNPPNVPHDYNPVGCYRHRFTLPENWKGKEIFIHFGA
                     VKSAFYLWINGKKAGYSEDSKTPAEFNITEYVQAGTNILAMEVYRWSDGSYLECQDFW
                     RISGIERDIYLVARPPVYIRDFFVHTALTDDYRNGEFKLETDIGTAGKKPGGYTLKVK
                     LLTSVGKITVADFSQEIKFSGKRESQIVFNQMIENPRKWTAETPDLYTLVLSVYNNKG
                     VCEESVSCKVGFKSYEMKNGQFLVNGIPVLIKGVNRHEHDPVTAHVISEESMLDDIRL
                     MKQFNINTVRTCHYPNDPRWYELCDQYGLYVIDEANIESHGMGYAPERTLGNNPAFMK
                     AHLDRVEAMLERDKNHPSIIFWSMGNEAGDGVNFDTCYKWIKKRDPSRPIHYERAELR
                     HNTDVYCPMYPDIEYLEEYASQPQARPLIMCEYAHGMGNSTGNLQDYWDVIEKYPQLQ
                     GGCIWDWVDQGFEKKNANGVVFFAYGGDYGPPETPSDSNFCINGLVLPDRTPHPALYE
                     VKKVYQYIDVRAVDPDRGIISVKNNYDFIPLTGFDIHWKLKGDGAVMAFGTLEQPDIL
                     PREEKSYSIPLPEIKPEPGVEYFLDFSMTIRQANGLIPEGHEVAAEQLPLTLKADPVM
                     MHDKAFLEIEWSKDRKKVDITGVDFLVRFDTLAGQITRLEYNGRNYISQGPLPNFWRA
                     PIDNDFGNRMPKRCAVWKEASLERTVKSFAAKKAAQGIAVISVIFELGKERFLYRMDY
                     EIHSTGDIIITGDINMDGRTTLPELPRFGMNFRIPAEFDHVKWFGRGPHENYQDRNTA
                     SFIGIYDKDVSEMYFPYVRPQENGTRTDIRWMTLTNPSGDGIMIAGMPLVSVSALPFT
                     TSQLDYTDNMFRHTTDLIPNYFIDINVDYMQTGVGGNDSWGARPLSKYTLFPSDYRFS
                     FRIRPINNKIDPAKAGKVVFSPLK"
     gene            5843..8218
                     /locus_tag="PKZ74_03455"
     CDS             5843..8218
                     /locus_tag="PKZ74_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HPM91842.1"
                     /translation="MKKILIPLIIISVSIFGCTGDKKEKATRPDIIPIPMNYSMGEGN
                     FTLNRKTDIFISLADNYLKDVAVLLQERIALLTGSEPEINILTGEQPKNSILLRLDGS
                     ISIEAGLEGYKLSVERSRIVISAPQPAGIFYGMQTIFQLLPPSAFTAGNSKAGLEDVK
                     WTVPCVEITDKPAFAWRGMHLDVSRHFFPREFILKYIDMIAMHKMNVFHWHLTDDNGW
                     RIQIDKYPLLTEVSAWRADRSDIPWNERAPQRPGEKATYGGFYTKEDIREIVEYARRR
                     FITVVPEIEMPGHTSEVFAAYPQFSCTGERTTVQTGSYWPNEDIFCAGKEETFRFIED
                     VLDEVIAMFPSPYIHIGGDEADKTAWKKCTDCQSRIRKEGLSGVDELQSYFVKRIERY
                     IKSKGRILIGWDEILEGGLAPDATVMSWRGFEGGIDAARQGHKVVMCPTSYCYFDYYQ
                     ANPDFQPPAIGGLITLKKVYSFRPKPSELSPEEQKLILGAQGNLWTEYVPEPDHAEYM
                     VLPRMTALAEVLWSPEPLLVWDDFKERLLTQNKRFGAMEANYFKGSGRVDATAVFNKD
                     DKPYAIKLETEKDGTKIYYTLDGYTPDKRSFMYKNPVAITNNVTLKAVAYKGDKQLEL
                     PVEYSIKFHKGVGKNVSYNLNYSERYTGGGGYALVDGLRGSLNYNDGFWQGWNGRNAD
                     FVISLGDEFYFKNISATFLLDQKRWIFYPEVVNFYVSDDGKNFQKIGSIRHLIDMNDP
                     AAQTNDFTVKISQPLKVKFLRIEAVNIGVCPDWHPYHGEKSWIFLDEVVLN"
     gene            8242..10191
                     /locus_tag="PKZ74_03460"
     CDS             8242..10191
                     /locus_tag="PKZ74_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91843.1"
                     /translation="MKKLFLIVLLLSGSLSGPAQADTSGYMAMPRLNYYTNEEVGEII
                     VITPGQLIGKKVTVDLVFEYEFLNRGFVVVPGALTTVPFEMKRLREGENEITVSFYEN
                     DKWTDSRKTYLTIRPHKENAVKVDQATGGLFVSGLPYFPAGFFAGFPVNPDLPGQEAV
                     NGFNMISPYQDLNKKTFKQRKAYMDRCANLGIRVNYNVSSGLYGGRDENCDQAGNEKP
                     VDKERLMKEILAMRDHPALLSWYIAEEPDSRDIPADSLAVYYHLIRELDPYHPVSMMV
                     STPRKADSYLGVTDIILINPNPIPYGTIREVYDYTRIAVGKSWPGKPVWIVPQAFGGN
                     RMWSREPNAAEVRAMTYMAIIHGATGVQFTLREPPNSFPKSTATWGECSRIAMELAEL
                     TSDLLSPHPAPRVLASEAGIHARAWNRRGMVTIAIVNEKNEPLNFSLEMEDFNVSVMA
                     DLLFENRKVAIVEGIIKDVIDGYGTRIYRFDARQRTGQEILPDPQNLTTDPGFEDMTT
                     AGVPSACYAYPGADPGCTYFTDPRRHYEGDHSLRMNNPSAGPGNLLSFYPVNVDSAMT
                     CTVSIMARSGPSPNKSGGKKGGPVRFRLAFGPAEQVFECTTAWEKYEIKGIKLDGAGP
                     AQVSPELELIGKGTAWFDLIQVVAE"
     gene            complement(10204..10701)
                     /locus_tag="PKZ74_03465"
     CDS             complement(10204..10701)
                     /locus_tag="PKZ74_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPM91844.1"
                     /translation="MIFHSLASEAVMRALARRLGRDEEQWGQAGLLHDLDVEITDADP
                     YVHGLETARILTEMGVDADLVEAIKLHNEMAASQPRTAEFHHALAAGETITGLIVATS
                     LVYPDKKIASVKPKSVVKRMKEKAFAASVRRENIMECELIGIPLDEFAEIALTAMTAL
                     ETANN"
     gene            complement(11165..12436)
                     /locus_tag="PKZ74_03470"
     CDS             complement(11165..12436)
                     /locus_tag="PKZ74_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPM91845.1"
                     /translation="MELLIILLLIVLNGVLSMSEIALVSAKKSRLQYMARKGNKQAAR
                     ALDLSNRPDRFLSAIQIGITLVGIFLGAYSGENITEDMQGILNRVTALQPYSHTLSFI
                     LVVLIITFFSLVLGELVPKQLGLAMPEKLAKAMAGPVHVFSVVTRPFVWLLSISTNLI
                     IKAFNIKASDDEKITEEEIKSMIEEGTRHGEVQDVERDIVDRVFILGDTTIKSLMTYK
                     SDIVWIDFSDEQEEIRQKVSANLHNVYPVAEGSLDQVMGTVGLKDLFTNLDKPGFSLA
                     NCIHDPFYLPENTSAYDAIRQFRLNKTFHALVIDEYGSVEGIITVSDILESLVGEVSP
                     ADEEYQIISRDDGTWLVDGQYPFHEFLNYFDLQELYEENRFNTIAGLILDQLGYIPKA
                     GDKTDWMGFRFEIMDMDRARIDKVLVSKEKP"
     gene            12578..13897
                     /locus_tag="PKZ74_03475"
     CDS             12578..13897
                     /locus_tag="PKZ74_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPM91846.1"
                     /translation="MSTRAYIISITIIGVFFFIFGFVTWLNGILIPYLKIACEISDFQ
                     SLFVAFAFYISYTVMALPSSWMLKKTGFKNGMSLGLAVMAAGSILFIPAAISRSFPLF
                     LTGLFVMGTGLAILQTASNPYITIIGPRETAARRISIMGICNKLAGAIAPLILAYFIF
                     SDGDAFIKSLEGMDNAAKAAALDNLAGRVIEPYAVMAAILLLLGLLIRFAPLPKVETE
                     EESPEELSAIAGKTNILQFPHLVLGAIALFFYVGIEVVAGDTIIRYGMSLGVPIESAK
                     YYTSLVLIAMVIGYIIGIILIPKILSQKNLLAVSAVTGIVFSALAIFVPASATMQFPF
                     FDLVTLQSIDIRLPYTVLFVALLGISNAIVWPAIWPLAIHGLGRFIKTGSAILIMSIA
                     GGALLPLLWGYLSDVWSAQQAYWIGIPCYLFILYFAVSGHKVGRKEV"
     gene            13930..14841
                     /locus_tag="PKZ74_03480"
     CDS             13930..14841
                     /locus_tag="PKZ74_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPM91847.1"
                     /translation="MEPTLLILAAGIGSRFGGLKQASGAGPSGEAILDYSIYDAVRAG
                     FKKVVFVIRRDIYDDMKKIFFDRWTHKIEIDYIFQELNDLPGGYTAPPDRVKPWGTGH
                     AIWVAREKISEPFCMINADDFYGRKSFELAAGFLRNMDNLIHSRYALIGYILKNTLSE
                     HGFVSRAECETDDTGKLTAITERLKIMREGESVIFSDESGAVMNISENAVVSMNMWGF
                     MPSLFRYLEEDLIRFLQENSTSAKAEFLLPNVIDRLIKSGEVDIPVMQTDSKWFGITY
                     KEDLEMVKGKLQKLVESGSYPSPIWIF"
     gene            14857..15906
                     /locus_tag="PKZ74_03485"
     CDS             14857..15906
                     /locus_tag="PKZ74_03485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPM91848.1"
                     /translation="MSNPLYNIAGLFNISSPAISIVRYGEGHIHDTYLVTTGSSQPDY
                     ILQKINHKIFRDIPGMMRNIKEVTDYLRFKNTGKSSTFSTPELVYTYEGQPFLELDEE
                     NFWRMYVYIPDTVTYQKMEDPALGFEAGKAIGRFQAGLAGLPVPLVETIPGFHDIFLR
                     IEQYYSARENDPLSRKGKIMDTIDFAEKRFDTAKNYLLQLREKGVIRATHNDTKLNNI
                     LFSRQNEALCLIDLDTVMPGYVHFDFGDALRTMANTALEDEPDLKRVRFNEKVYQSVT
                     KGFLAEAGPMLNEAEKELLPYAPVYMTFIIGLRFLADYLNGDTYFRIHHPDHNLDRAR
                     VQFKLVQEIEQFLKA"
BASE COUNT         4218 a         3617 c         3896 g         4182 t
ORIGIN      
        1 atatacggta tcctcctggg cctgatcctc tttgccggcg ctacgttcca acaggtaggc
       61 attgtctata caacggccgg aaatgccggt tttattaccg ggctctatgt gattctcgta
      121 cctgtttttg gaatcttttc cgggcaaaag gcacgtgtca acctctgggg aggggctctg
      181 atcgctacgg tggggatgta tttcctttcg gtgacagaag ccttttcgat ttcaaaaggc
      241 gatctgtttg ttctgacagg cgccgttttc tgggcggtgc acgtactgta tacagggtgg
      301 gtcagtccga aagtgtcagc aatcaggctg gccgcagttc aatatgcggt gtgcgccctg
      361 ctgagcctgt tcgctgcatt gatttttgaa aagaataccc tttcaggtat ttacgatgcc
      421 gcgatcccat tgctgtatgg cggttttctt tcggtcgggg tggcttttac gctgcagatt
      481 gtcggacaaa agaaagcgcc gccggctcac gcagccatta ttctgagcct ggaagcagtg
      541 tttgcagtag tcggaggcat gttgatcctg tcggaaacca tgaccgaccg caaatggttc
      601 ggctgtgccc tgatgcttgc aggcatgctg ctggcgcagg ttaaaagcag gcagtcggca
      661 gtcagcagtc agcagtcggc agtaagcagt aagtatggtg aaaattgaaa cttgaaaata
      721 gaaatttgaa acccggaacc tggaacccgg aacctggaac ccggaacctg gaacctggaa
      781 cctggaacct ggaacctgga acctggaacc tggaacccgg aacaaaacct aaacacatga
      841 acctaaaact agaagtctgt gccagttcgc tgacatcagc cttgaatgcc caggctggcg
      901 gggcgcaccg gatcgagctc tgcgataacc tgaatgaagg agggactact ccctcggctg
      961 gaacggtcat tctagcaaaa aaactcctgg gaattccggt atttgttctg atcaggccga
     1021 gggccggcga ttttacttat tcagatgtag aatttgaatc gatgcaagag gacatcctgt
     1081 tttgcaaacg gaatggcctc gatggcattg tcaccggaat tttgaccgga agcggcaatg
     1141 tagatatcga cagaatgcga atcctggtcg atctggcccg gcccatgcag gttactttcc
     1201 accgggcatt cgatttgact ccggatccta ttaaagcgct tgaagagatc atcagccttg
     1261 gcatcgaacg tattcttact tccggacagg ccgaaaatgc actggccggc gctaagctga
     1321 tcaaacaatt gaacaatcga actaataaca atatcatcat tatgcccgga ggaggagtga
     1381 ctgaaaacaa tattcatctt cttgctaatc aaacaggtac ccaggaggtg catgcttctt
     1441 taaggtctcc ggttaatagc agcatgtgta accggaataa aacggtttcg ataaagaaaa
     1501 acccctcgga cgactattcc tggatggaaa cagatcggcg tcgggtaaaa aaaatgtacg
     1561 aattaatgac aaagttgtac gaataaatgc tttgcgattt ttttaagtta ttgaaatata
     1621 agatattaaa aatttgcgat attttgttga attttttgtc ctttgccgtt attgaatttt
     1681 gatttcaggc attgtatatt tgccaaacaa tcctgaaaat ctaactttaa aactataact
     1741 atgaaaaaat tctacatttt attgattatg atgctgactt tcagcggctt tgcgttcagt
     1801 caggtcagtt cacgaatgga taatcctcct gctccggtca tggcctcacc ggaaagtcac
     1861 gttactcgcg cttcaggatg taactttctg gtttttatgg ataatcttcc atggaatagc
     1921 agtgccattc agacaattct gaccgccaat ggagaatcct gggtgttgca aaattctgca
     1981 tcgatggcat caaccgattt ttctatgtat gatgtgatca tcattgcttc cgatcagata
     2041 ccttcatttt atgataattt tgcggctaat tttgcgaagt ttgtaacttt tgttaccaac
     2101 ggaggatcgc ttgaggttca tgcagctacc tgcggatgga attctccatg cggctactct
     2161 gttctgttac caggaggtgt ttttaccacg gagcaatacg atgattataa cctcgttgtt
     2221 gacgctgccc atccgattgt agccggagta tcaagcccat ttagtggtag ttatgcaagc
     2281 cacggttact tctcaaacct tgttgccggc acggatatta taacagaaac ctcttccaac
     2341 aataagccca cgaccataca atacgtttat ggagcgggaa tcgtcactgc tactacctgt
     2401 acttatgaat tcggctatga ctttggtcag gaagtaggtg agatgctggt caacaacctg
     2461 aactacggat gtgaacatgc catcgacgct aaggtgccgg tttccgactg ggccatcgcc
     2521 atcggtatct tcatgatcct ttttgccgct gtattgcgct acagaagagt attctaataa
     2581 tagatattta aatatttgca gagagtgtct cgtttaccgg ggcactcttt tttttatttt
     2641 tatccaaaat atccgcgata attcatttct atgcataaaa gttatctttt attctttagt
     2701 ttcctgttct catttatctg ccaggcacag caaattcccg agtgggaaaa tccccaggtt
     2761 acgggtgtaa ataaagttca gcctcatgcc actcttattc cctatcctga tgagagaacc
     2821 gccatcgaaa atgtgaagtc aaagtcccct ttttatctcc ttctgaacga tgaatggaaa
     2881 ttcaactggg tcgaaaaacc attcgatcgc ccggccggtt tctacaatcc tgattttgac
     2941 gattcgggct ggaataacat ccctgtccct gctaattggg agttgctggg atacggtgtc
     3001 cccatctacg ttaatatatc ttatgaatgg acccgtaatc ctaatccgcc gaacgtaccg
     3061 cacgactata atcccgtcgg atgttaccgc caccggttta ctctccctga aaactggaaa
     3121 ggcaaagaga tatttattca tttcggggcg gtgaaatctg ctttttacct ctggatcaac
     3181 ggaaaaaagg ccggctacag cgaagatagc aaaacccccg ctgaatttaa tattacggaa
     3241 tatgtccagg caggcacaaa catcctggcg atggaagtgt accgttggtc cgacggatca
     3301 taccttgaat gccaggactt ctggcgtatc agcggcatag aaagggacat ttatcttgta
     3361 gcccggccac cggtttatat acgcgacttt ttcgtccata cagcacttac agatgactat
     3421 cggaacggtg aattcaaact cgaaacagac attggaactg ccggtaaaaa gccgggcggt
     3481 tacacattga aagtcaaact gttgacatca gtcggcaaaa tcaccgttgc agatttttca
     3541 caggaaatca aattctcagg taaaagagaa tcacagatcg tctttaatca aatgattgaa
     3601 aatccacgaa agtggaccgc tgaaacaccg gatttatata cactggtttt gtcggtttac
     3661 aataacaaag gcgtttgtga agaatcggtc agttgtaaag tgggttttaa atcatatgag
     3721 atgaaaaacg gacagttcct ggtcaacggt atcccggtat tgatcaaagg ggttaaccgc
     3781 catgaacacg atccggtaac ggctcatgtg atttcggaag agtcgatgct tgatgacatc
     3841 cggttgatga agcagttcaa tatcaatacg gtacgcacct gccattatcc gaatgatccc
     3901 cgctggtatg aactgtgtga ccaatacggg ctttatgtga ttgatgaagc aaatatcgaa
     3961 tcacacggga tgggatatgc tccggaacgg acccttggca acaacccggc attcatgaaa
     4021 gcgcacctcg acagggtgga agccatgctg gaacgcgata aaaaccatcc gtccataatt
     4081 ttttggtcga tgggtaatga agccggcgac ggtgttaatt tcgatacatg ctataaatgg
     4141 attaaaaagc gtgatccttc ccggccgata cattatgaac gtgcggaatt gcgccataat
     4201 accgatgtgt actgcccgat gtaccccgat attgaatatc ttgaagaata tgcatctcaa
     4261 ccacaggcac gtccgttgat catgtgtgag tatgcccacg ggatgggcaa cagtaccggc
     4321 aacctgcagg attactggga tgtgatcgaa aagtatccgc aactccaggg gggctgcatc
     4381 tgggactggg tcgaccaggg attcgaaaag aagaatgcga acggagtagt ttttttcgca
     4441 tacggaggcg attatggccc gccggaaacg cccagcgaca gcaacttctg catcaacggc
     4501 ctcgttttgc ccgaccggac gccccatccg gctctttacg aggttaaaaa agtgtaccag
     4561 tatatcgacg ttagagctgt agatcccgat aggggaatta tttcagtgaa gaataattat
     4621 gactttattc ccctgacggg attcgatatc cattggaaac tgaaaggcga cggagcggtt
     4681 atggcattcg gcaccctgga acaacctgat atcctgccgc gtgaagaaaa atcttattct
     4741 ataccgttgc cggaaatcaa accagaaccc ggcgttgaat atttccttga tttcagcatg
     4801 accatccgac aagccaatgg cctgatcccc gaaggtcacg aagtggccgc ggaacaactg
     4861 cctctgactt taaaggccga tcctgtgatg atgcatgata aggcatttct tgagatcgaa
     4921 tggagcaaag acagaaaaaa ggtggatatt accggggtgg atttccttgt aagatttgat
     4981 accctggccg gccagatcac ccggctggaa tataacggcc gcaattatat ctcacaagga
     5041 ccgctgccaa acttctggcg tgcgccaatc gacaatgact ttggtaaccg tatgcccaaa
     5101 cggtgtgccg tctggaaaga ggccagcctt gaacgaacgg ttaaatcgtt tgcagccaaa
     5161 aaagcagcac aaggtattgc ggttatatcg gtgatatttg aattagggaa ggagagattc
     5221 ctttaccgta tggattatga aatacatagc acgggcgata ttattattac cggtgatatt
     5281 aatatggatg gaagaacaac cctgcccgag cttccgcggt ttggaatgaa tttcaggatc
     5341 ccggcggaat ttgaccatgt gaagtggttt ggcagagggc cgcacgaaaa ttaccaggac
     5401 cgcaataccg cttcttttat tgggatttat gacaaagatg ttagcgagat gtatttccct
     5461 tatgtccgcc cgcaggagaa tggcacccgt accgatatcc gctggatgac actgacaaat
     5521 ccttccggcg acgggatcat gatagccggg atgccgctgg tatcggtctc tgcattgcca
     5581 ttcacaacca gtcagcttga ttatactgac aatatgttcc gccatacgac cgatttgatt
     5641 cccaactact ttatcgatat taatgtcgat tacatgcaga cgggagtagg agggaatgat
     5701 agctggggcg cacggccatt atcgaaatac acccttttcc cttccgatta ccggttcagc
     5761 tttcgcatcc gtccgatcaa taataaaata gacccggcta aagcgggtaa agttgtattt
     5821 tcgccactga aatgaaaaga atatgaaaaa gatactgata cctctgatta taatttcagt
     5881 ttctattttt ggatgtacgg gtgataaaaa ggaaaaagca acccggcccg atattatccc
     5941 gatacccatg aactacagca tgggtgaagg taactttacc cttaaccgga aaaccgacat
     6001 ttttatcagc ctggcagata attacttaaa ggatgtggca gttctgttgc aggaaaggat
     6061 agctttactt accggatcgg aacccgagat taatatcctg acgggtgagc agcccaaaaa
     6121 ctcaatcctg ctgcgcctgg atggctctat ttcaattgaa gccggcctgg aaggatataa
     6181 attatcggtc gagcgttcaa ggatagtgat cagtgcaccg caacccgccg gtatctttta
     6241 cgggatgcag acgatctttc agcttttacc cccttcggcc ttcacagccg gtaatagcaa
     6301 agcgggactg gaggatgtga aatggaccgt tccttgtgtt gagattacag acaaacccgc
     6361 ttttgcctgg agaggtatgc acctggatgt ctcccgccat ttctttccca gggaattcat
     6421 cttaaaatat atcgatatga tcgccatgca caagatgaat gtctttcact ggcatcttac
     6481 cgatgataat ggctggcgta ttcaaatcga taaatacccg ctgctgacgg aggtctcggc
     6541 ctggagagcc gatcgcagcg acattccctg gaacgaaagg gcgccgcagc gaccgggcga
     6601 gaaagcaacc tatggcggtt tttataccaa ggaagatatc cgggagatcg ttgaatatgc
     6661 caggcggcgg ttcataacgg tcgtacctga gattgaaatg ccgggccaca cctcggaagt
     6721 gttcgctgct tatccgcaat tttcatgcac aggcgagaga acaaccgtac aaaccggatc
     6781 ctactggccg aatgaggata ttttttgtgc aggtaaggag gaaactttcc ggtttattga
     6841 agatgtcctg gatgaggtca tcgcaatgtt cccatcgcct tatatacata tcggaggcga
     6901 tgaagccgat aaaacggcct ggaagaaatg caccgactgt cagtcgagga tcaggaagga
     6961 aggactctca ggcgtggatg aactgcaaag ctattttgtg aaaaggatcg agcgttacat
     7021 aaaatccaaa ggacgaatcc tgatcggctg ggacgagatt ttggagggtg gtcttgctcc
     7081 tgatgccacc gtgatgtcgt ggagaggctt tgaaggaggg atcgatgctg ccaggcaggg
     7141 gcataaagtg gtcatgtgcc cgacatctta ttgctatttc gattattacc aggcaaatcc
     7201 cgattttcaa ccccctgcta tcggtgggtt gattaccctg aaaaaggtct attcattcag
     7261 gccaaagcct tcagaacttt ctccggagga acaaaaacta attctcgggg cccagggaaa
     7321 tctttggaca gaatatgtcc ctgaaccgga tcatgctgaa tatatggttc ttcccagaat
     7381 gacggccctc gcggaagtcc tgtggagccc cgagccttta ctggtttggg atgatttcaa
     7441 ggaacgcctg ctgacccaga ataagcgctt tggtgcgatg gaagcgaatt attttaaagg
     7501 gtcgggaaga gtagacgcta ctgcagtttt taacaaggat gataaaccct atgccatcaa
     7561 acttgaaacg gaaaaggatg gcactaaaat ttattatacg ctggatgggt acacacccga
     7621 taaaaggtcg ttcatgtata aaaatcccgt tgccattacg aataatgtta ctttgaaagc
     7681 cgttgcctat aaaggggata aacagctgga actgccggtg gaatatagta ttaaattcca
     7741 taaaggagtg ggcaaaaacg tttcgtataa tttgaattac agcgaaagat ataccggtgg
     7801 cggcggttat gccctcgtcg atggccttag gggatcgctt aactataacg atggattctg
     7861 gcagggttgg aatgggagaa atgccgattt tgtcatttcc ctgggcgatg aattttattt
     7921 taaaaatatt tcggcaacct ttcttctgga tcaaaaacga tggatctttt acccggaagt
     7981 ggtcaatttt tatgtgtcgg atgatggtaa gaattttcag aagattggat ctatccgtca
     8041 tttgatcgat atgaacgacc cggctgccca gaccaatgac tttacggtaa agatcagcca
     8101 acccctgaaa gttaaatttc tcaggataga agcggtgaat attggagtat gcccggactg
     8161 gcatccctat catggcgaga agtcctggat atttcttgat gaagtggttt tgaattagtt
     8221 catgaatttg attattgcag aatgaaaaaa ctattcttga ttgttctcct gctgtcggga
     8281 agcctttcgg gaccggcaca ggctgatacc tcaggctata tggctatgcc caggctgaat
     8341 tattatacaa acgaagaggt tggagagata atagttatta ctcccggaca attgatcggg
     8401 aaaaaagtaa ctgttgacct tgtctttgaa tatgaattcc tgaacagggg atttgtagtg
     8461 gttcccggag ccttaaccac cgtacccttc gagatgaaga ggttaaggga aggcgaaaat
     8521 gagattacgg tatctttcta cgaaaatgat aaatggaccg attccaggaa gacatatctg
     8581 accatcaggc cccacaagga aaatgcggtc aaggtggatc aggcaacagg tgggctgttt
     8641 gtttccgggc tcccttactt cccggctggt ttctttgccg gctttcctgt taatccggac
     8701 ttacccgggc aggaggctgt gaatggtttc aatatgattt caccttacca ggatttgaac
     8761 aaaaagacat ttaagcaacg gaaagcttat atggaccgtt gcgctaatct cggaattagg
     8821 gtcaactata acgttagcag tggtttatat gggggaagag atgaaaactg cgatcaggcg
     8881 ggaaatgaga agccagttga taaggaacgg ttgatgaaag agattctggc catgcgcgac
     8941 catccggcgc ttttgagctg gtatattgcc gaagaacctg acagtcggga tattccggct
     9001 gattcgctgg cggtgtatta ccatcttatc agggaacttg atccctatca cccggttagc
     9061 atgatggttt ctactccacg aaaggccgat tcctacctgg gcgtcaccga tattatcctg
     9121 atcaacccga atcccattcc ctacgggact atccgggagg tttacgatta tacgaggatt
     9181 gcggtcggta aatcctggcc cggcaaaccg gtttggattg taccgcaggc cttcggtggc
     9241 aacaggatgt ggagccgcga acctaatgca gcggaagtcc gggctatgac ctatatggct
     9301 attatccacg gagctacggg tgttcagttt actttgaggg aaccgccaaa cagctttcca
     9361 aaatcgacgg caacatgggg tgaatgtagc aggatagcca tggaactggc tgaattgact
     9421 tccgatctgc tttcaccaca tccggcgccg agggttctgg ccagtgaggc aggtattcac
     9481 gcccgggcat ggaacaggcg cggaatggtc accattgcca tagttaatga aaagaatgag
     9541 ccgttgaatt tttcgctgga gatggaagat ttcaatgttt cggtgatggc ggatcttttg
     9601 tttgagaacc ggaaagttgc catcgtggaa ggaattatta aagatgtgat tgatggctat
     9661 ggtacccgga tttaccgttt tgatgccagg cagagaaccg ggcaggagat cctgcctgat
     9721 ccgcagaatc tcacgaccga tccgggtttt gaggacatga ccacagccgg agtaccttcg
     9781 gcctgttatg cctatcccgg ggccgatccc ggatgcactt atttcacgga tccccggcgc
     9841 cattacgaag gcgatcattc cctgaggatg aataatcctt ctgccgggcc cggaaatctg
     9901 ctgagttttt acccggtcaa tgtcgattcg gccatgacct gtacggtcag cattatggcc
     9961 cggagcggac cctcgccgaa taagtccggt gggaagaaag gcggcccggt aaggttcagg
    10021 cttgctttcg ggcctgcaga gcaggttttc gagtgcacaa cagcctggga gaaatatgag
    10081 attaagggaa ttaagctgga tggggccggt ccggcacagg tttctcccga attggagttg
    10141 attggcaaag gaacagcctg gttcgacctc atacaggtcg ttgccgaata agcaggtttc
    10201 cgctcaatta ttggctgttt caagtgcagt catggcagtt aatgcaatct ccgcaaactc
    10261 atccagcgga atccctatca gctcacattc cataatattc tcccgcctga cagatgcggc
    10321 aaaagccttt tccttcattc ttttaacaac gctcttcggt ttgacgctgg cgattttttt
    10381 atcaggatag accagggaag tagccacgat caggccggtg atcgtttcgc cggcagccag
    10441 ggcatgatgg aactccgcag tgcgtggctg gcttgcagcc atttcgttgt gtaactttat
    10501 cgcctcaacc aaatcggcat caacgcccat ttcggtaagt atccgggctg tttcaagccc
    10561 atggacatac ggatcggcat cggtgatttc aacgtcgagg tcgtgcagca gtcctgcctg
    10621 cccccattgt tcctcatctc ttccgagcct gcgggctaat gcacgcataa cagcttcaga
    10681 ggcaagagaa tgaaagatca tccgttcgtt tttaatgtgc tgatgcagaa ggcctattgc
    10741 ttgttcgcgg ggtaaaagca tggtgtgttt ttatgcaaat ttagtcaatt tggaaatgag
    10801 acaatttgaa aatgagacaa tttgaaaatg agacaatttg gaattcattg gacaatggac
    10861 aatggaaaat attttacccc ggaggggttc cacatttgta gtgattagga atgcccatag
    10921 caatctcccc tcccttgttg ttcccggatc aaggtgttcg ggtctgcacg ggaggggacg
    10981 ggggtgggga aaggaaatca tttcgcattg tccattgtcc atttcgcatt gtccatagaa
    11041 gaatgatagt catcagacaa tgcaaaatga aaaacagaac atgccatgca aaatgaaaaa
    11101 tgcaaaacgc aaaatgcaaa accacctgga aaatggccaa tggaaaatgc gcaatggaaa
    11161 atgcttatgg cttttctttg ctcaccagca ccttatcgat ccgcgcccgg tccatatcca
    11221 tgatctcaaa ccggaatccc atccaatcgg tcttgtcgcc ggctttggga atgtatccca
    11281 actggtcgag gatcagcccg gcaatggtgt tgaaccggtt ctcttcgtaa agctcctgca
    11341 aatcgaaata attcaggaac tcatggaaag gatactgccc gtcgacaagc caggtgccgt
    11401 cgtcgcggct gatgatctgg tattcttcat ccgccgggga tacttctcct accagcgatt
    11461 ccaggatgtc gctcaccgtt atgatccctt ccacacttcc gtattcgtcg atcaccaaag
    11521 catgaaatgt cttgttcaac ctgaattgcc ggatagcatc atacgctgat gtattctccg
    11581 gaagataaaa gggatcatgg atacagtttg ccagcgaaaa gccgggtttg tcaaggttgg
    11641 tgaaaaggtc tttaaggcca acggttccca tcacctggtc gaggcttcct tcagcaaccg
    11701 ggtaaacatt atgcagattg gctgaaactt tctgcctgat ctcttcctgt tcatctgaaa
    11761 aatcaatcca gacaatatca cttttgtaag tcattaacga tttgatggtt gtatcgccga
    11821 gaatgaaaac ccggtcgacg atatcccttt ccacatcctg cacctctccg tgccttgtcc
    11881 cttcttctat catcgatttg atctcttctt ccgttatctt ctcatcatct gaagctttta
    11941 tgttaaatgc tttgatgatc agattggtgg agatgcttaa cagccagaca aacggcctgg
    12001 tgactactga aaaaacatgg accggacctg ccattgcttt tgccagtttt tcaggcatag
    12061 ccaggcccaa ctgcttagga accagttcgc ccagcaccag ggagaaaaat gtgatgatca
    12121 gcacaaccag gataaacgac aaggtgtgcg aatagggctg aagcgcagtg acccggttaa
    12181 gaattccctg catatcttct gtaatattct cgccgctgta tgctcccaga aatatcccta
    12241 ccagcgtgat ccctatctgt atggccgaca gaaaccggtc aggacggttg ctcaggtcca
    12301 acgccctggc tgcctgttta ttccctttgc gtgccatgta ttgcagcctt gatttcttcg
    12361 ctgaaacaag agcgatttcc gacatggaaa gcacgccatt caacactatg agcagtaaaa
    12421 taatcaggag ttccattctg gaattagaat tgaacggcaa agttacggaa agaaatcttt
    12481 ataaatttgc atccaggcaa tgaataatga tttcttaaat tattcacatc aagaatttta
    12541 aattaagaat taagaattta acattaaaca ttatcccatg tccacccggg cttacattat
    12601 ttctatcacg ataatcggcg tctttttttt tattttcggg tttgtcacct ggctcaacgg
    12661 gatcctgatc ccgtatctca aaatcgcctg tgaaatatct gattttcagt cgctctttgt
    12721 ggcttttgct ttctacatct cttacaccgt aatggcctta ccctcgtcat ggatgttgaa
    12781 aaagacaggc tttaaaaatg gaatgagttt aggccttgcg gtcatggcag cagggagcat
    12841 tttattcata ccagcggcca tatcgcgttc tttcccattg tttcttacgg ggcttttcgt
    12901 catgggaacg ggcctggcta tcctgcaaac ggcttccaat ccgtacatta cgatcatcgg
    12961 gccccgggaa acggccgcac gccgcatcag tatcatgggg atctgtaaca agctcgcagg
    13021 ggccatagcc ccgctcatcc tggcttattt tatatttagc gatggcgatg ctttcattaa
    13081 aagccttgaa ggaatggata acgcagcaaa ggctgcagcg ctggataacc tggcaggaag
    13141 agtcatcgag ccgtatgccg taatggcggc tattcttctt ctgctcgggt tattgatccg
    13201 ttttgcccct ttgccaaagg tcgaaaccga agaagaaagc ccggaagaac tttcggctat
    13261 agccggcaaa accaatatct tacaatttcc tcacctggta ctcggagcta tcgccctctt
    13321 cttttatgtt ggaatcgaag tcgttgcggg cgatacgatt atacgttacg gcatgtcgct
    13381 cggagtaccc atcgaatcgg ccaaatatta cacctccctg gtgctcatcg ctatggttat
    13441 cggttatatc atcgggataa tcctgatccc gaagattctt tcacaaaaaa acctgctggc
    13501 tgtcagtgca gttaccggca tcgtcttctc ggccctggcc atctttgtcc ctgcatccgc
    13561 caccatgcag ttcccgtttt tcgacctggt aaccctgcaa agtatcgaca tccggctgcc
    13621 ttatacggta ttgttcgtgg cgctgctggg tatctccaat gcgatcgtat ggcctgccat
    13681 ctggcccctg gccattcacg ggctcggacg gtttatcaag accggctcgg ctatcctgat
    13741 catgagcatt gccggcgggg cgctcctgcc tctgctctgg ggttaccttt cggatgtttg
    13801 gtcggcccag caagcctatt ggatcgggat tccatgctat ctgttcatcc tgtatttcgc
    13861 cgtttcaggg cataaggtgg gcaggaaaga ggtgtaaggg gtggaatatt gaatttggaa
    13921 tttttatata tggaaccaac cttattaata ttagcagccg gtatcggaag ccggtttggc
    13981 ggacttaaac aggcatccgg cgcagggccg tcaggagaag cgattctcga ctattcaata
    14041 tatgatgccg tgagagccgg atttaaaaaa gtagtctttg taatccgccg tgatatctac
    14101 gatgatatga aaaagatctt tttcgatcgg tggactcaca aaattgaaat agattatatt
    14161 ttccaggaac tgaatgatct gccgggtggt tacacggccc cgcctgacag ggtaaagccc
    14221 tggggaacag gacacgccat ctgggtggcc agggaaaaga tcagtgaacc tttctgcatg
    14281 atcaatgccg atgattttta tggaaggaaa tctttcgaac tcgccgccgg ttttctcagg
    14341 aatatggata acctcatcca cagccgttat gctctcatcg gttatatcct gaaaaatacc
    14401 ctctcggaac acggttttgt ttcccgtgcc gaatgtgaaa cagatgacac aggaaagctc
    14461 accgcgataa cggaacggct taaaataatg agggaaggag aatcggtcat tttcagcgat
    14521 gaatcgggtg cagtaatgaa tatcagtgag aacgctgtcg tttcgatgaa tatgtgggga
    14581 tttatgcctt cgcttttccg ttacctggaa gaagacctca tccggttcct tcaggaaaat
    14641 tcaaccagcg ccaaagctga atttctgctt cccaatgtca tcgacaggct aatcaaatcg
    14701 ggtgaagtgg acattcctgt catgcaaacc gattcgaaat ggttcgggat cacatacaaa
    14761 gaggatctcg aaatggttaa aggtaagttg caaaagctgg tagaaagcgg aagctatcct
    14821 tcgccaatct ggatattctg aggagccgcg atacggatga gcaacccttt atacaatatt
    14881 gcaggtctat tcaatatttc ttccccagcc atatcgatag tccgatacgg agaagggcat
    14941 atccatgata cttacctggt caccactgga tccagccagc ccgattatat tcttcaaaag
    15001 atcaatcata agatcttcag ggatattccc gggatgatgc ggaatatcaa agaagtcacg
    15061 gattatctcc ggttcaaaaa caccggaaaa agcagtactt tttcaacacc cgaactggtt
    15121 tatacttatg aaggtcagcc gttcctggag ctggatgagg aaaatttctg gaggatgtat
    15181 gtttatatcc cggatacggt aacttatcag aaaatggagg atcccgcttt aggatttgag
    15241 gccggtaaag ccatcggacg tttccaggca ggtctcgccg gtttgccggt accattggtg
    15301 gaaaccatcc ccggttttca tgatatcttc ctccggatag agcagtatta ctcggccagg
    15361 gaaaatgatc cgttatcccg caagggaaaa ataatggata ctattgattt tgctgaaaaa
    15421 cgatttgata cggcaaagaa ttacctgctg caactgagag aaaagggggt tatccgggca
    15481 acccataatg acaccaaact gaacaacatc ctgttcagcc gtcagaatga agcgctgtgc
    15541 ctgatagatc tcgatacggt aatgccggga tatgtccatt ttgactttgg cgacgctttg
    15601 cggaccatgg ccaatacagc cctggaagat gaacccgacc tgaaacgggt tagattcaat
    15661 gaaaaagttt atcagtcggt gacaaagggg ttcctggctg aagcagggcc aatgctgaat
    15721 gaggctgaaa aagaattatt gccgtatgct cctgtataca tgacgtttat catcggtctc
    15781 cggttcctcg ctgattatct gaacggggat acctatttcc ggattcacca cccggatcat
    15841 aatctggacc gggccagggt acagtttaaa ctggttcagg agatagagca atttttgaaa
    15901 gcctgaggct gtt
//
LOCUS       DAOPNH010000039        15719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3684_length_15719_cov_6.467314, whole genome shotgun sequence.
ACCESSION   DAOPNH010000039 DAOPNH010000000
VERSION     DAOPNH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3684_length_15719_cov_6.467314"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3231)
                     /gene="sprA"
                     /locus_tag="PKZ74_03490"
     CDS             complement(<1..3231)
                     /gene="sprA"
                     /locus_tag="PKZ74_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPM91849.1"
                     /translation="MRRVGSNIFTFSLISLIVLLSWTIPASVDYGHTHGFDIPWFSPP
                     DTTVKGDTSVVLPFPPPKQDDYLLYQPEDTGGLYLRPPKNFKTEVEYDPSTNQYYFRN
                     KIGDLDYRNPTYMSFDEYQEYELDNTIKQYWRERSSPSSTLTKDGIIPSLYIGGQVFD
                     RIFGSNTIDIRPQGSAELSFGVLANRRDDPSLNVRQQRTTNFDFEQNIQMNVMAKIGD
                     KIQFNTNFNTEAVFDFENKLKLNYEGKEDEIIKLIEAGNVSMPLSTTLITGTQSLFGF
                     KTKLQFGRTTVTGLFSEQESESSTVVIENGKESNRFELKATDYEENKHFFISHLFRES
                     YDKALAELPIVASDINITRIEVWVTNIGPAIQQNRNLIAFQDLGEFARINNPNVNPIP
                     GRPYPSNNSNNLLQQLDVNQLRSISSVGTYLSGDPFSIGYTGYMVNGEDYEKIESARK
                     LETSEYSVNSKLGFISLYTTLNPDQSLAVAFQYTVIGVDSVFQVGEFSDQTSNTSNCL
                     VVKLLKSTSLSTQVPMWDLMMKNVYSIGAYRVSRDKFILNILYSGNSNGVPTGYFTEG
                     PEGIKGTPLIQFFNLDNLDQLLNPPPDGLFDFIDNAAVSGGTMNSSNGRLYFTVIEPF
                     GKFLRNKFGPGNTDLANKYAFDTLYRSTKTIAEQQTEKNKYLLEGFYSSESGAEIDLN
                     AFNIPRGSVKVTAGGRTLTENVDYTVDYTLGRVRIINEGILNSGLPINITNENQSTFN
                     VLKKRMMGLRVDHQIGKDIRFGGTLLNLTERPLTQKVDYGNDPISNTIWGLDGSYQGE
                     SRLITSIVDKLPGIETKAPSKVNVEGEFASFMPGHSKAIGKSGVVYIDDFEGAKSTID
                     LRNVGTWFLASTPQGQPDLFPEASGNDRSYGFNRAKAAWYTIDPLFYDRNNNLRPANI
                     SRNELARHSVREVLETEVFPNKEIPNGIPTNIPVFNLAFYPSERGPYNYDVDGLPGIS
                     AGIGQDGSLIDPASRWGGIMRKIESSDFEATNVEYIEFWMMDPFNSDAEQNNPGRLYI
                     NLGDISEDILKDGRKFYENGLPTSEIVTDVDLRAVPADIGDGAGGLA"
     gene            complement(3322..3933)
                     /gene="ruvA"
                     /locus_tag="PKZ74_03495"
     CDS             complement(3322..3933)
                     /gene="ruvA"
                     /locus_tag="PKZ74_03495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPM91850.1"
                     /translation="MYEYIQGKLAEINPAFAILDVGGVGYNLQITLNTYTRISNLDQC
                     RLYAHLVIREDAQVLFGFAETEERDLFRLLLTVSGIGPNTARLLLSSLNIVELKDAIT
                     AGRVNILKSVKGIGEKSAQRIIVDLRDKIDKGSSPVQKVDFSHNTIREESLSGLVILG
                     FPRKIAEKAVDDVIRELKQLTSEGSPTTVINVERIIKEALKRL"
     gene            4079..6376
                     /locus_tag="PKZ74_03500"
     CDS             4079..6376
                     /locus_tag="PKZ74_03500"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPM91851.1"
                     /translation="MDNLFSKQALEYHSQGRPGKLEVIPTKPYSTQKDLSLAYTPGVA
                     KPCLEIKANPDDVYKYTMKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGLLFKVFA
                     DLDVFDIEINEKDIDKFVQTVVAIAPTFGGINLEDIKAPECFEIEDRIKEALDIPVMH
                     DDQHGTAIITAAGLLNALEITGKKIENLKVVVNGAGASAISCTRLYLALGVKRENVTM
                     VDTKGVLRSDRTDLNKYKRFFATDRDVHTLEEALAGADMFLGLSAAGALKKEWLKTMA
                     DHPVVFAMANPIPEILYDDAVEAREDIIMATGRSDYPNQVNNVLGFPFIFRGAMDVMS
                     TKINEEMKLAAAHALAALAKEPVPEEVNIAYAHKNLKFGKEYIIPKPLDPRLILWVAP
                     AVAKAAMDSGVARRPITNWDAYKDELSKRMGLSNPLTRQIRTRAAHDIQKVVFAQAEN
                     YNMCKAAEIVMNERLAMPILLGNVARIKKIIQDNELELEGVEIIDPRSDQEQARRHQF
                     AQILYEKRQRFGVTVPTAEEQLIHRNYFAPMMVETGQADAMISGLVRNYPDTLNPAIE
                     VIGKKPGARLVSGMYIINSKKGSFFFADCSVNKNPTMEDLIEITLQTVYAVEQFKIKP
                     RVAFVSYSNFGSNPGRVPDMQRDAVAYLHKHYPNLVVDGEMQVNVALDPEIQKEHFPF
                     SKLADGPANVFIFPYLTAGNIAYKLLQVIADAEVIGPVINGMNKSVHVLQIGSTVTEI
                     VNMVCVSAIDAMCVKARMAGEERCR"
     gene            6544..7485
                     /gene="mdh"
                     /locus_tag="PKZ74_03505"
     CDS             6544..7485
                     /gene="mdh"
                     /locus_tag="PKZ74_03505"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPM91852.1"
                     /translation="MNKITVIGAGNVGATVANVLAQKDLAREVVLLDIKEGVAEGKAL
                     DIWQTSSILNFNTRVRGCTNDYLKTKGSGVIVITSGIPRKPGMSRDDLIKTNATIVKE
                     VVERSIRYSPDAIILIVSNPLDVMTYVAYKAANKESRKVFGMAGILDTGRYKAFIAEA
                     LDISPKDIHALLMGSHGDTMVPLRRYTSVGGIPVTELMNKEELDKIIERTRKGGGELV
                     DLMGTSAWYAPGAAVAEMVEAIVDDQKRVFPVCAYLNGEYGQKGIYLGVPVKLGKNGI
                     EEVIQLQLNKNEMKMVEDSANSVKEVMAVLDDMKLFD"
     gene            complement(7482..9038)
                     /locus_tag="PKZ74_03510"
     CDS             complement(7482..9038)
                     /locus_tag="PKZ74_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPM91853.1"
                     /translation="MDRTESFKQKINAGYACQGGAIIMGSAMLDGEGVPGALVKIPLK
                     TMNRHGLIAGATGTGKTKTLQIIAEQLSENGVPVLLMDIKGDLSGIARPGTINQHIEK
                     RHSIMGEPWEGKGSPVEFLSLSDEKGARMRATVIEFGPVLLSRILDLNDIQAGVVSLA
                     FKYCDDNGIPLLDLKDFKKVLQYLSNEGKDEIKEEYGAVSSTSVSTIMRKIIEIEQQD
                     ADRFFGEESFNVDDLIRIDRNGKGIVSILRLTDIQDRPKLFSTFMLSLLAEVYNSFPE
                     VGDAEKPKLVLFIDEAHLIFNEASKALLSQIETIARLIRSKGVGIYFCTQNPTDVPDV
                     ILGQLGLKIQHALRAFTPKDRQMIMKTAENYPESEFYNVPDLLTSLGMGEALITALDE
                     KGRPTPLAATLLRSPMSRMDVLTNEEIDEIIDNSKLVPFYNREIDRESAFEILTARIE
                     SRQSSDVSRQSGEAKGRVGEEKGRKGEKEAPSVFEEISKNPMARQIGRTLVSELARGI
                     FGMLGGRRRR"
     gene            9152..9847
                     /locus_tag="PKZ74_03515"
     CDS             9152..9847
                     /locus_tag="PKZ74_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPM91854.1"
                     /translation="MKKIAVLTGAGVSAESGLKTFRDHDGLWHDHRIEDVATPEAWER
                     DMDLVNQFYNERRKNLLEAMPNEAHIALARLEEKYNVTVITQNVDDLHERGGSSNVLH
                     LHGELKKVRSTVDPELVYELNGWELKRGDLCEKGSQLRPHIVWFGEAVPLIEDAAGIV
                     QEADIVIIIGTSLQVYPAAGLIHYAFAGAPKYYIDPKAVQVPFIPMLEIIRKPAGEGV
                     PELVDKLMLNVEF"
     gene            9853..10314
                     /locus_tag="PKZ74_03520"
     CDS             9853..10314
                     /locus_tag="PKZ74_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPM91855.1"
                     /translation="MPEEQDLILAKLRSFCAYQERCIHDVTEKLEEWRIGQKRAEKII
                     EKLIKEDYLNEERFARTYAGGKFRINHWGKTKIIYELEKRQVPDLIIQIGLQEIDDQE
                     YKETLKEILTRKAREIKEKDPYRRRQKLTAYAVQKGYEYHLIREVAEQMNR"
     gene            10554..11646
                     /gene="prfB"
                     /locus_tag="PKZ74_03525"
     CDS             join(10554..10616,10618..11646)
                     /gene="prfB"
                     /locus_tag="PKZ74_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131840564.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPM91856.1"
                     /translation="MITQEFYKNLNQRLDALRRFLDVEKKKQTITEKEELTHAQDFWN
                     DPKKAEEVLRSIRDIKRWTDAFDEAKASFDDLVVIYEFYEAGEATDEDLDRQAEKTTK
                     IIENLEFLNMLSREEDKLSAILQINSGAGGTESQDWVAMLMRMYIRWAEIQGHKVKEL
                     DRQDGDVAGIKSVSIEIDGEYAFGYLKGENGVHRLVRLSPFDSANRRHTSFASVYVYP
                     MVDENIQVEINPADITWDTFRSSGPGGQNVNKVETAVRLKHEPTGIIVECQETRSQLQ
                     NREKAIQMLKSQLYEIELRKKREAQAEIESKKKKIEWGSQIRSYTLHPYKLVKDHRTD
                     LEAGNAQAVLDGEIDEFLKAFLMEFGGSQ"
     gene            11722..13113
                     /gene="fumC"
                     /locus_tag="PKZ74_03530"
     CDS             11722..13113
                     /gene="fumC"
                     /locus_tag="PKZ74_03530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPM91857.1"
                     /translation="MNTRTEKDTMGLVEVPAEKYWGAQTQRSVMNFKIGDPGSMPVPI
                     IRAFGILKKAAALTNFELGVLPKEKCDIICQVCDEIIAGKLDDNFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLLGNKLGEGTRFIHPNDDVNKSQSSNDTFPAAMSIAGYKMIVET
                     TIPGVEKLKQTLEEKSAGFMDIVKTGRTHLMDATPLTLGQEFSGYAAQLGYGIKALKN
                     TLPHMSELALGGTAVGTGINTPKGYSELVAKKIAELTGFPFVTAPNKFESLSAHDAVV
                     ESSGALKTLAVSLMHIASNIRLLSSGPRCGYSEIILPANEPGSSIMPGKVNPTQPEAM
                     TMVCAQVLGNDVAINIGGTHGHFQLNVFKPVMIANLLQSARLLGDACVSFNDHCVIGI
                     EPNLPNIKKNMENSLMLVTSLNTKIGYDNAALIAKKAHTENKTLREAAIESGLVTGEQ
                     FDEWVDPRKMIGR"
     gene            13286..14524
                     /locus_tag="PKZ74_03535"
     CDS             13286..14524
                     /locus_tag="PKZ74_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91858.1"
                     /translation="MIERKFYLGKIKSGFAVVPIVLLIGARQVGKTSLMKMFNFNGAS
                     LFLNGQDPEISGLFERLNSIEDYIKVYLNPDLTGLLMIDEFQFIPGISTMLKLLTDKN
                     EGLKILCSGSSSLDIQQTVEESLAGRIRVIEVLSLSFEEYLKFTREKLHMLYENFGLT
                     TPDSALTAPFHQALKEYMLYGGLPRAALIHDYEQKLAILDDIYKTYLVRDVRSYIKNE
                     HFTGFNRLLKMLASQVGNMVNINSISRETGLPYRACDEYMDLLQQMYIIKLIAPFSSN
                     KRSAITKMKKIYFTDLGLRNMISSGFQEMEYRADKGALFENFILLELFRNLKPGGNIY
                     YFRTTDGTEVDFIVQNPTSSYAVECKYAILQKPLKIRALNSLSGDENLINCYIINQNF
                     NFSEGTTHHIQGYLAGFINR"
     gene            complement(14685..>15719)
                     /locus_tag="PKZ74_03540"
     CDS             complement(14685..>15719)
                     /locus_tag="PKZ74_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91859.1"
                     /translation="GESVGNNASLKVTWGDGTSNIYTSDSEGRIHILRENTLDTARNI
                     FIENADTTYFQEWIFGVKQENLITTKEKNLFQSQKEQSGAPGTSYVAPTPAPANLTMF
                     PDTFEVYFVSKIVYDSEGQAHNTRGEVFRTVVSCRSNPDLTKKWEIGQNVTTYLYEMQ
                     TYESKKNAIDPKTFDLGKYLEEQFSNRETLEYITPEQSALQSQALDSLIAQGFYLRQN
                     GRQLFPEFERVLVYDGTEPEYLEGQARGWDQTHFSQYGNNNENGCVFTSDFTFSDYYR
                     FKGAIANYTSTSNMSYKMDELIGSFTHSRDPPNGNLSGKVTFTDGHLTSFGNGVIHVI
                     YIADPGTIFW"
BASE COUNT         4206 a         3677 c         3866 g         3970 t
ORIGIN      
        1 cgcgagtcct ccagccccgt cacctatgtc tgcaggcacg gcacggagat cgacatcggt
       61 cactatttca gaggttggaa ggccgttctc ataaaacttc cgtccgtctt tcaaaatatc
      121 ctcagaaata tcgccgaggt tgatatatag tcttccggga ttattttgtt cggcatcgct
      181 gttgaacgga tccatcatcc agaattcgat atattccaca ttcgtcgctt caaaatcgct
      241 cgattcaatt ttgcgcatga tcccgcccca ccggctcgca ggatcgatca gggagccatc
      301 ctgtccgata ccggcagaaa tacccgggag cccatccaca tcatagttat aagggcccct
      361 ttcggaagga taaaaagcaa ggttgaaaac cgggatattg gtcggaatcc cgttggggat
      421 ctccttattc gggaagactt cggtttccag cacctccctg acggaatggc gggccagttc
      481 attgcgtgaa atattggccg gcctcaggtt attattccgg tcatagaata aaggatcgat
      541 cgtgtaccat gctgctttgg cccggttgaa tccatagctt ctgtcgttac ctgaagcttc
      601 agggaacagg tcgggctggc cctgcggggt actggccagg aaccaggtgc cgacatttcg
      661 aaggtcaatg gtcgatttag ccccttcaaa atcatcgata tacactacgc cgcttttacc
      721 gatagctttg gaatgtccgg gcataaaact ggcaaactca ccttcgacat taacttttga
      781 aggggctttt gtttcgatgc cggggagctt atcgacgatg ctggtgatca gcctcgattc
      841 accctggtaa cttccgtcca gtccccagat agtatttgaa atcgggtcat ttccgtagtc
      901 gaccttttgt gtcagaggcc tctcggtcag gttcagcaac gtacccccga accggatatc
      961 cttcccgatc tggtgatcga cccgtaaacc catcatcctc ttcttcaaaa cattgaaggt
     1021 cgactggttt tcattcgtaa tattgatcgg aaggcccgaa ttgaggatac cttcattgat
     1081 gatcctcacc ctgcctagcg tatagtcaac cgtatagtcc acattttcgg tcagcgtgcg
     1141 gccaccggcc gtcaccttca ccgacccgcg gggaatgtta aaagcgttca gatcgatttc
     1201 cgcacccgac tccgaagaat agaatccttc cagcaggtac ttgttctttt ccgtttgttg
     1261 ttcggcgatg gttttcgtgg agcggtaaag cgtatcaaaa gcatatttat tggccagatc
     1321 ggtgttaccg ggcccgaatt tattcctcag gaacttgccg aatggctcaa tgacggtaaa
     1381 ataaagcctt ccgttggatg agttcatcgt acctccgctg acagcggcat tgtcaatgaa
     1441 atcgaacaat ccgtccggcg gtgggttgag cagctgatcc aggttatcca ggttgaagaa
     1501 ctggatcagc ggtgttcctt tgatcccttc cggtccttcg gtaaaataac cggtcgggac
     1561 accgttgctg ttcccggaat agaggatatt taaaatgaat ttatcgcggc tgacccggta
     1621 agcgccaatg gaataaacat ttttcatcat caggtcccac atcggcacct gggtactgag
     1681 ggaggtgctt ttcagcagtt ttacgaccag gcagttggag gtgttggagg tctggtctga
     1741 aaattccccg acctggaaga cgctatcgac gccgatgacc gtatactgga aagccactgc
     1801 cagcgactgg tcggggttaa gcgttgtata aagcgaaatg aaaccgagtt ttgaattgac
     1861 cgaatattcc gaagtttcaa gtttccgggc gctctcgatc ttttcataat cttccccgtt
     1921 gaccatataa cccgtgtaac cgatgctgaa agggtctccg ctcaggtaag ttccgaccga
     1981 actgatgctg cgcaactggt tgacatcgag ctgctggagc aggttattgg aattatttga
     2041 cggataaggc cttccgggta taggattgac attcggatta ttgatgcggg cgaattctcc
     2101 caggtcctgg aatgcaatga ggttacggtt ctgctggatg gccggaccga tattggtgac
     2161 ccaaacttca atacgggtga tattgatatc ggatgcgaca atgggcagct cggccagggc
     2221 tttgtcataa ctttcacgga agagatgtga gatgaagaag tgtttgttct cctcatagtc
     2281 ggtcgctttc agctcgaacc ggttcgattc ctttccgttt tcaatgacga ccgtagaact
     2341 ttcgctttcc tgttctgaaa acaggccggt caccgtcgtc cggccgaact gcagtttggt
     2401 tttgaatccg aagaggctct gcgttccggt gatcagggtc gtgctgagag gcatcgacac
     2461 attcccggct tcgatcagtt taatgatttc atcctctttt ccttcgtaat taagcttgag
     2521 cttgttttca aaatcgaata cggcttcggt attgaaattg gtattgaact ggatcttatc
     2581 accgattttg gccatcacgt tcatctggat attctgctca aaatcgaaat tggtggtccg
     2641 ttgctgcctg acgttcagcg acgggtcatc gcgccggttg gccaggactc cgaatgaaag
     2701 ctcggctgag ccctgcggac gaatgtcgat ggtattgctt ccgaagatcc ggtcgaaaac
     2761 ctgtcccccg atgtaaaggc tcgggatgat cccgtctttg gtgagggtgc tgctgggcga
     2821 cgacctttct ctccagtatt gtttgatcgt attgtcgagt tcatactcct ggtattcgtc
     2881 gaacgacata taggtcggat tccggtaatc gagatccccg attttgtttc ggaagtaata
     2941 ctggtttgtc gagggatcgt attcgacttc ggttttaaaa ttcttcggag gcctgagata
     3001 cagtccaccg gtatcttctg gctggtatag cagataatcg tcctgtttcg ggggagggaa
     3061 tggaagcaca accgatgtat cgccttttac cgtagtatcg ggaggtgaga accagggaat
     3121 gtcaaaaccg tgcgtatggc catagtccac actggcaggg atagtccatg acaggaggac
     3181 gatcaggctg atcaggctga atgtaaaaat atttgatcct actctccgca aacgttatcc
     3241 tggtttatgg cctgttaaaa acaaaattga aaacccgggt aaagttttca atgtaagata
     3301 cgatactgtt taccgctatt ttcaaagcct tttaagtgct tctttaatga tccgttccac
     3361 attgatgaca gtggtggggc ttccttcgga agtcagttgc tttaactccc tgatcacatc
     3421 atccactgct ttttcggcta tctttctcgg aaaacctaaa ataaccagtc ctgataacga
     3481 ttcctcccgg atagtattgt gtgagaaatc aactttttgg acaggcgatg atcctttgtc
     3541 aattttatcc cttaagtcaa caatgatccg ctgtgccgat ttctccccga ttcctttgac
     3601 cgatttgaga atattgacac gtcctgccgt aatggcatct tttaattcaa caatattcag
     3661 tgatgaaaga agaagccgtg ccgtattggg gccgatgccg gaaaccgtca gcagcagccg
     3721 gaacagatct ctttcttccg tttcagcaaa tccgaagaga acctgtgcat cttcccggat
     3781 aaccaggtgg gcatacaacc tgcattggtc aagattcgat attctggtat aggtattcag
     3841 ggtgatctga aggttatacc ctactccgcc gacgtcgagg atggcaaagg cgggatttat
     3901 ttcagccagc tttccttgga tatattcgta catatcagtt cgttccgggc tcttttttcg
     3961 gagggcaaaa ataggaattt aatttagaac taatctgcct aaaccatcaa taaagaaata
     4021 agatgaaaat attatatttg ctgattgtgt taatttctta acaataaatt aaaaaccaat
     4081 ggataacctc ttcagcaaac aagccctgga atatcactcc caggggaggc cgggcaaact
     4141 agaagtgatc ccgaccaaac cttacagtac ccaaaaagat ttgtcgctgg cctatacacc
     4201 gggggtagcc aaaccctgcc tggaaatcaa ggccaacccc gacgatgtat acaaatacac
     4261 catgaaaggg aacctggtgg cggtgatctc gaacggaaca gccgttctgg gcctgggcga
     4321 tatcggcccg gaagccggaa agccggttat ggaaggcaaa ggattgctgt ttaaagtttt
     4381 cgcggatctt gatgtttttg atattgagat caatgagaaa gatatcgata aatttgtcca
     4441 gaccgttgtt gccattgcgc caacctttgg cgggattaac ctggaggata tcaaagcgcc
     4501 ggaatgtttt gagatcgaag accgcatcaa ggaagcgctg gatattcccg ttatgcatga
     4561 tgatcagcat ggaaccgcaa ttattacggc agccggtttg ctgaatgcat tggaaatcac
     4621 gggtaaaaag atcgaaaatc tgaaagtcgt cgtcaatggc gccggggcat cggccatttc
     4681 atgcacgagg ctttaccttg cactgggagt gaaacgggag aatgtgacca tggtcgatac
     4741 caaaggcgtg ttgcgcagcg accggaccga tctgaacaaa tacaagcggt tctttgcaac
     4801 ggacagggat gttcatacat tggaagaagc gctggcagga gccgacatgt tcctgggcct
     4861 ttcggcggca ggcgcactga aaaaagagtg gctgaagact atggccgacc atccggttgt
     4921 tttcgccatg gccaatccca tcccggaaat actttacgac gatgctgtcg aagctcgtga
     4981 agacattatc atggctaccg gcagatcgga ttatccgaac caggtcaaca atgtgcttgg
     5041 cttccccttc atatttcgcg gagcaatgga cgtgatgtcg accaagatca acgaggagat
     5101 gaaactggcg gccgcacatg cactggcagc gctggccaag gaaccggttc cggaggaggt
     5161 gaacatcgcc tatgcgcata aaaatctgaa gttcggcaaa gaatacatta tccccaagcc
     5221 tctcgatccg cggctgatcc tctgggtggc cccggcagtg gcaaaagcag ccatggattc
     5281 aggtgttgcc cgtagaccta tcaccaactg ggatgcttat aaagacgaac tgagcaaaag
     5341 gatgggcctg agtaacccgc tgacacggca gatcaggacc cgggctgcac acgatataca
     5401 aaaggtcgtt tttgcccagg ctgaaaacta caatatgtgc aaagccgccg aaattgtgat
     5461 gaacgaacgc ctggccatgc ccatcctgct gggtaatgtg gccagaataa agaagatcat
     5521 ccaggacaat gaactggaac tggaaggggt ggaaattatc gatccccggt cagaccagga
     5581 acaggcgagg cgacatcagt ttgctcaaat cctttacgaa aagaggcagc gttttggcgt
     5641 tacggtgccg acggctgagg agcaactgat acaccgcaat tactttgcgc caatgatggt
     5701 tgagacgggc caggccgacg ccatgatatc ggggctggta agaaactatc ccgacactct
     5761 caatccggcc atcgaagtca tcgggaaaaa gccgggggcg cggctggtat cgggcatgta
     5821 tatcatcaac agcaagaaag gatccttttt ctttgccgac tgttcggtga acaagaaccc
     5881 gacgatggaa gacctgatcg agatcacgct gcagacggtt tatgcagtag aacagttcaa
     5941 gatcaagccc agggtagctt ttgtatccta ttccaatttc ggctccaacc cgggccgggt
     6001 ccccgacatg cagcgcgatg cagtcgctta tcttcacaag cattatccca acctggtagt
     6061 agatggtgag atgcaggtga acgtagccct ggatccggag atccagaagg agcacttccc
     6121 attctccaag ctggccgacg gcccggcaaa cgtgttcatc ttcccctatc ttacggcagg
     6181 aaatatagcc tacaagctgc tacaggtcat tgccgacgcc gaagtgatcg gcccggtgat
     6241 caacggcatg aacaagtcgg tccatgtcct gcagatcggc agcaccgtca ccgagatcgt
     6301 gaacatggtg tgcgtcagcg cgatcgatgc gatgtgtgtc aaagccagga tggcgggaga
     6361 agagagatgc aggtgagatc aaagttcaaa gttcaaggtt caaggtttta agttttagat
     6421 tctttgttcg ggattcaaag ttgggattgc gatattaatt atatcacaaa tatttctatt
     6481 tttgcatact tgaatctttc caggttgaac acgaatcgta aaaaaaataa aaaaaacatc
     6541 cttatgaata agattaccgt catcggcgcc ggaaatgtcg gtgctaccgt agcaaatgtc
     6601 ctggctcaga aagatctggc cagagaagtc gtattactgg atatcaaaga aggggtagcg
     6661 gaaggcaaag cgctcgacat ctggcagacg tcatccatcc tgaattttaa cacccgtgtc
     6721 aggggatgta caaatgatta ccttaagacc aaaggctcgg gcgtgatcgt gataacatcg
     6781 ggcattccgc gaaaaccggg tatgtcgcgc gatgatttga tcaagaccaa cgcaactatc
     6841 gttaaggaag tcgtagaaag gtccatccgg tattctcctg atgcgatcat cctgatcgtt
     6901 tccaatcctc tggatgtgat gacctatgtg gcctacaaag cggccaacaa ggaatccagg
     6961 aaagtcttcg gaatggcggg tattctcgat acgggacgtt acaaggcttt tatcgccgaa
     7021 gcgctcgaca tttccccgaa agatatccat gctttgctga tgggaagcca tggcgacacg
     7081 atggtgccgt taaggagata cacttcggtc ggaggcatcc ccgttaccga gctgatgaac
     7141 aaagaagaac tggataagat catcgagcgg acccgcaaag gcggcggcga actggtcgac
     7201 ctgatgggca cctccgcctg gtatgctccg ggcgctgcag tagccgaaat ggtcgaagcc
     7261 atcgtcgacg accagaaaag ggttttcccg gtttgcgcct acctcaacgg cgaatacggc
     7321 cagaaaggca tttacctcgg agtgccggtg aaactgggca aaaacgggat cgaggaagtg
     7381 attcagctgc aattgaacaa gaatgagatg aagatggtgg aagattccgc caattcggtg
     7441 aaagaggtga tggctgtttt ggatgatatg aaattgtttg attaacgccg ccggcgccct
     7501 cccagcatcc cgaaaattcc cctggccagt tccgacacca acgtccggcc aatttgccgc
     7561 gccatagggt ttttactgat ctcttcgaaa acagacggtg cttccttctc cccttttctc
     7621 cctttctcct ctcccactct tccctttgcc tcgcccgact gacgactgac atctgacgac
     7681 tggcgacttt ctatcctggc cgtcagtatt tcaaaagcgc tctcccggtc aatttcccga
     7741 ttataaaacg ggaccagttt agagttatcg atgatctcat cgatctcttc attggtcaaa
     7801 acatccatcc ggctcatggg ggaacgcaaa agggtagccg cgagaggcgt tggccggcct
     7861 ttctcatcca aagcggtgat cagcgcctca cccatcccca gcgaagtgag caggtcgggc
     7921 acattgtaaa actccgattc gggataattc tcagcggttt tcatgatcat ctgccggtct
     7981 ttcggtgtga aagcccgcaa agcatgctga atcttcaacc cgagctggcc caggatcaca
     8041 tccggaacat cggtggggtt ctgcgtacag aaataaatac ccaccccttt cgaccggatc
     8101 agcctggcga tcgtttctat ctggctgagg agggctttgg aagcttcgtt gaagatcagg
     8161 tgggcttcgt cgatgaacag caccagcttc ggtttttccg catcgccgac ctcggggaag
     8221 gaattgtaaa cctccgcgag aagcgaaagc atgaatgtcg aaaacaattt cggacggtcc
     8281 tggatatcgg tcagccttaa aatcgacacg atcccttttc cattccggtc gattcttata
     8341 agatcatcga cattgaacga ttcttccccg aagaaccggt cggcatcctg ctgctcaatt
     8401 tcgatgatct ttctcatgat ggttgaaaca gacgtcgatg atacagcgcc gtattcttcc
     8461 ttgatttcgt cttttccttc attggaaaga tactgcagca ctttcttgaa gtctttcaga
     8521 tccagcaggg ggatcccgtt gtcatcgcaa tacttgaaag ccagcgaaac gacgccggcc
     8581 tggatatcat tcaggtcgag gatacgggat aacagaaccg gtccgaactc aatcactgtc
     8641 gccctcatgc gggccccttt ctcatccgac aggctgagaa actcgaccgg ggatcctttt
     8701 ccttcccagg gttcacccat gatcgaatgc cgcttctcga tatgctggtt gattgtcccc
     8761 ggcctggcaa tgccgctcag gtcgccttta atatccatta acaacaccgg caccccgttt
     8821 tccgataact gctcggctat gatctgaagc gtcttggtct tcccggttcc cgtagcgccg
     8881 gcaatgagcc cgtggcggtt catggttttc aggggaatct tcaccagcgc ccccggtacg
     8941 ccttctccgt ccagcattgc actgcccatg atgattgcac caccctggca ggcatagcca
     9001 gcatttattt tttgtttgaa tgattcggtt ctgtccatcg gatgcatatt ttcgacaaaa
     9061 ataataatac gtgggtatgt tagagacgca atattttgcg tctctaacaa atccacgaaa
     9121 aaaattgtaa ttttatagaa aacaaaacca aatgaaaaaa attgctgtcc tgaccggcgc
     9181 cggcgtcagt gcggaaagcg ggctgaagac attccgcgat cacgacggcc tgtggcacga
     9241 ccaccggatt gaagatgtgg caacgccgga agcctgggaa cgggatatgg acctggtcaa
     9301 ccagttttat aacgaaagaa ggaaaaatct tctggaagcc atgcctaatg aggcacatat
     9361 tgcgctggcc cggctggaag aaaaatacaa cgttacggtg atcacacaga atgtggatga
     9421 ccttcatgaa aggggaggtt catccaatgt tttgcatttg cacggagaac tgaagaaagt
     9481 caggagcacg gtcgatccgg aactggttta cgaattgaat ggatgggagc tgaagcgggg
     9541 cgatttatgc gaaaaaggat cccagttgcg ccctcatatt gtctggttcg gtgaagcggt
     9601 tccgctcatt gaggatgcgg cgggaatagt gcaggaggct gatattgtga tcattatcgg
     9661 aacgagtcta caggtttatc cggctgccgg gttgatccat tatgcatttg ccggggcacc
     9721 taagtattac atcgatccta aagccgtaca ggtgccattc atccccatgc tggaaataat
     9781 ccggaaaccc gctggcgaag gggtgccgga actcgttgat aaacttatgc ttaatgttga
     9841 attttgaagg ttatgcctga agaacaagat ttaatcctcg caaaactccg cagcttctgc
     9901 gcataccagg agcgctgcat ccacgatgtg accgaaaagc tcgaagaatg gcggatcggg
     9961 caaaaaaggg cggaaaagat cattgagaag ctcatcaaag aagattacct caatgaagaa
    10021 cgctttgcca ggacctatgc cggcggcaag ttccggatca accactgggg caagacgaaa
    10081 atcatttacg agctggaaaa aagacaggtg cccgacctga tcatccagat cgggctccag
    10141 gaaatcgacg accaggaata taaagaaacg ctgaaggaga ttctgactcg caaagcaagg
    10201 gaaatcaagg agaaggatcc gtatcggcgc aggcaaaagc taactgctta tgcggttcag
    10261 aaggggtatg agtatcattt gataagagag gtggctgaac agatgaaccg gtgaaacgaa
    10321 tcagcaaaac cctaaggcat gttcatattt aagttgtcac agtatgtttc tcggtctatc
    10381 ttgagatggc atctcaaatc catggattta attcatatat cattctatat cttctcttta
    10441 caacgatttt ccgtagattt gagatgccat ctcaaaaaat ctcgctagct aggtttaatc
    10501 cccttgagat taataactat ctttacctca taatacaatc ttaaatatgc gttatgatca
    10561 cccaggaatt ttataaaaat ctgaatcaaa ggcttgatgc tttgaggagg ttcctttgac
    10621 gtcgaaaaga agaaacaaac catcacggaa aaggaagaac tgacgcacgc gcaggatttc
    10681 tggaacgatc ccaaaaaagc ggaagaggtt ttacgttcca tccgggatat caaacgatgg
    10741 acggatgcct tcgatgaggc taaagcatct tttgacgacc tggttgtgat ctatgaattt
    10801 tatgaagccg gggaagctac ggatgaagac ctggaccggc aagcggaaaa gaccacaaaa
    10861 attatcgaga acctggagtt cctgaacatg ctcagccggg aagaagacaa gctgagcgcc
    10921 attctccaga tcaactccgg ggcaggcggt accgaaagcc aggactgggt ggccatgctg
    10981 atgcggatgt atatccgatg ggccgagata caaggccata aagtcaagga gctcgaccgt
    11041 caggacggcg acgtggcagg gatcaaatcg gtttcgatag aaattgacgg cgaatacgcc
    11101 ttcggctacc tgaaaggcga aaacggggtg caccggctgg tccgcctctc cccgtttgat
    11161 tcggccaacc ggcgccatac ttcttttgct tcagtgtatg tttacccgat ggtggatgaa
    11221 aatatccagg tcgagatcaa tccggccgat ataacctggg acaccttccg ttccagcggt
    11281 ccgggcgggc agaatgtgaa taaagtggag acggcggtcc ggttgaaaca cgagccgacc
    11341 gggattatag tggagtgcca ggaaacccgc tcacagcttc agaaccgcga aaaagcgatc
    11401 cagatgctga aatcgcagct ctacgagata gaactcagaa agaagagaga agcccaggcc
    11461 gagatcgaaa gcaaaaagaa aaagatcgag tggggatcac agatacgttc atataccctt
    11521 catccttaca agctggttaa ggaccaccgc accgacctcg aagcaggcaa tgcccaggct
    11581 gtgctcgacg gggagatcga cgaattcctg aaagcctttt tgatggagtt cggcgggagt
    11641 cagtaggcag ttggacggtt ggacagttgg acagtataac aatttatcaa tttaacaatt
    11701 tatcaaacca aataacaacc catgaacaca agaacagaaa aagacacgat gggcctggtg
    11761 gaggtgccgg ccgaaaaata ctggggagca cagacccagc gatctgtcat gaacttcaaa
    11821 atcggtgatc cgggatcgat gccggtaccg atcatccggg ctttcggcat cctgaaaaaa
    11881 gccgcggccc tgaccaattt cgaactgggc gtcctgccga aagaaaaatg cgatatcatc
    11941 tgccaggtat gcgatgagat catcgccgga aaactcgacg acaatttccc gctggtgatc
    12001 tggcagaccg gttcagggac gcagtcgaac atgaacgtaa acgaagtggt agccaaccgg
    12061 gcccatgttt tgctgggcaa taaactcggc gaagggacac ggttcatcca cccgaatgat
    12121 gatgtgaaca agtcgcagtc gtcgaacgat acgtttccgg cagccatgag catcgccggg
    12181 tataaaatga tcgtggaaac gacaattccc ggtgtggaaa agctgaaaca gacgctggaa
    12241 gaaaaatcgg ctggtttcat ggatattgta aagaccggcc gcactcacct gatggacgcc
    12301 acaccgctga cgctgggaca ggaattctca ggctatgcgg cacagcttgg ctatgggatt
    12361 aaagcgttga agaacacgct tccgcacatg tcggagctgg cgctgggcgg aacagcggtc
    12421 ggaaccggga tcaatacgcc gaaaggttac tcggagctgg tggccaagaa aatagccgaa
    12481 ctgaccggat tcccgttcgt gacagcgccg aacaagttcg aatccttatc ggcccacgac
    12541 gcggtagtcg aatcgagcgg cgcactgaag accctggctg tcagcctgat gcacattgca
    12601 agcaacatcc gtctcctctc ctccgggccc cgatgcggct acagcgagat catcctgccg
    12661 gccaacgaac ccggctcgtc gatcatgccc ggcaaggtca acccgaccca gcccgaagcg
    12721 atgacgatgg tctgcgcaca ggtgctcggt aacgatgtcg ccatcaatat tggcggcacg
    12781 cacggccatt tccagctgaa cgtcttcaaa ccggtcatga tcgccaacct gctgcaatcg
    12841 gcccgcctgc tgggcgacgc ctgcgtctca ttcaacgacc attgcgttat cggcatcgag
    12901 cccaatcttc cgaatattaa gaagaacatg gaaaactcac tgatgctggt aacctcactg
    12961 aacaccaaga tcggctacga caatgccgcc ctgatcgcca agaaggcgca taccgagaac
    13021 aagacgcttc gcgaagcagc gattgaaagc ggtctggtaa cgggagaaca gtttgatgaa
    13081 tgggtggatc cgaggaagat gattgggaga taacgatttt atcgtaattc ccggataaaa
    13141 tttccggatc ttataaaaat attgaaaatc acaagttatt taccataaac gtaaaatata
    13201 ttgctataat attatcatta atgtaaaata actggattta aatcattatt taatgtatct
    13261 ttaccaggat tttaaaactt gaataatgat tgaaagaaaa ttctacctgg gtaaaataaa
    13321 aagcggtttt gccgtagtac ctattgtttt gctcatcgga gcccggcagg ttgggaagac
    13381 cagcctgatg aagatgttta attttaatgg cgcatcgtta ttcctgaacg ggcaggatcc
    13441 ggaaatatcc gggttgtttg agagactgaa ttccattgaa gactatatta aggtctacct
    13501 gaatcctgat ttgacaggac tactaatgat cgatgaattt caattcatcc cgggcatttc
    13561 tacaatgctt aaattactca cagataagaa tgaaggattg aaaatccttt gttcaggtag
    13621 ttcaagtctt gatattcagc agaccgtgga agagtcactt gccggaagaa tcagggtaat
    13681 agaagtctta tcgctttcat ttgaggagta ccttaaattt accagggaaa aacttcatat
    13741 gctttatgaa aattttgggc ttactacgcc tgattctgca ttaacagcgc catttcatca
    13801 agcacttaaa gaatatatgc tttatggtgg attgccacga gctgcgctca tccatgatta
    13861 tgagcaaaaa cttgccatcc tggatgatat ttataaaact taccttgttc gcgatgttcg
    13921 aagctatatt aaaaacgagc atttcacagg atttaacagg ctgttgaaga tgttagcttc
    13981 ccaggttgga aatatggtga atattaattc gataagtcgt gaaacaggtc tgccttacag
    14041 ggcttgtgac gaatacatgg atttactgca gcagatgtat attattaaac tgattgcgcc
    14101 tttttcatct aataaacgtt cggcaattac aaagatgaag aaaatatatt tcactgatct
    14161 gggattaaga aatatgatca gctctggttt tcaggaaatg gagtatcgtg ccgataaagg
    14221 agcgcttttt gaaaatttta tcctgttgga gctttttaga aacctgaaac ccgggggcaa
    14281 catttactat ttcaggacaa ctgatggaac cgaagtggat tttatagtgc aaaatccaac
    14341 ttcaagctat gctgtggaat gtaagtatgc aatccttcaa aaacctctta aaatccgcgc
    14401 tcttaatagc ttaagtggcg atgaaaattt aataaactgt tatattatca atcaaaattt
    14461 caatttcagc gaagggacaa cacaccatat ccagggttac ctggctggat ttataaatcg
    14521 ttgatcagga ccgaacacag gcggaactgt tctcacaggc gctccaagct gcacatcaaa
    14581 ctgcatgaat gtagacacaa caacctgcac ctgcagttta ccaatagctg aaggacaacc
    14641 atattacgta gactaaataa aattattttt tattctatta taatttacca gaatattgtg
    14701 ccagggtctg caatataaat aacatgaata accccattac caaagctagt aagatgacca
    14761 tctgtaaaag taacttttcc actcaaattg ccatttggag gatcccttga atgtgtaaat
    14821 gaccctatta gctcatccat cttataactc atattgcttg ttgaggtata atttgctatt
    14881 gcacctttaa atctataata atcactgaat gtaaaatctg atgtaaaaac gcacccattt
    14941 tcattattgt ttccatattg cgagaagtga gtctggtccc atcctctggc ttggccttcc
    15001 aagtattctg gttctgttcc atcataaacc aacactcttt caaactcagg gaacaactgg
    15061 cggccatttt gtcgcaagta aaatccttgt gcaattaaag aatcaagtgc ctgcgactgc
    15121 aaagcacttt gttcaggagt aatatattcg agtgtttctc tattgctaaa ttgctcttct
    15181 aaatatttac ctaggtcgaa ggtttttggg tctattgcat tcttctttga ttcatatgtc
    15241 tgcatctcgt aaagataagt tgttacattc tgtcctattt cccatttttt agttaaatca
    15301 ggatttgatc tacaagagac tactgttctg aagacttctc ctcgcgtatt atgtgcctgg
    15361 ccttctgagt cataaactat ttttgaaaca aaataaactt caaatgtgtc tgggaacatg
    15421 gttaaatttg ctggcgcagg agtaggtgca acatatgacg ttcctggtgc gcctgactgt
    15481 tctttctggc tttgaaatag gtttttttct ttggttgtta ttaggttttc ctgttttact
    15541 ccgaatatcc attcctggaa gtaggttgtg tctgcgtttt ctatgaaaat attccgagca
    15601 gtatccaatg tgttttctct taaaatatga attcttcctt cagaatctga agtataaatg
    15661 ttacttgttc cgtctcccca ggttactttt aatgatgcgt tgtttcctac tgattctcc
//
LOCUS       DAOPNH010000040        15322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3861_length_15322_cov_6.103164, whole genome shotgun sequence.
ACCESSION   DAOPNH010000040 DAOPNH010000000
VERSION     DAOPNH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3861_length_15322_cov_6.103164"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2128)
                     /locus_tag="PKZ74_03545"
     CDS             complement(<1..2128)
                     /locus_tag="PKZ74_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91860.1"
                     /translation="MITPSFIPKSVSVLAIVIILLGSSAAFSQYPVVTVRFNNPEYVC
                     ATQTYSLDVEMQCDSANKRLHGMNIRFFYDDDVLEFTSFTEIIAAYGLTIPNPPEITT
                     DSTGLSMELFGIEGNYEYVNGAVKVVSSTPVTYLPTNGWTKIFAVNFHVDDPEVFQDS
                     TFCPAVIWDLQEDPSLGGMMGGGGVCITLVKIYPNITSPATEHVNQFNWQYDYIPGLP
                     YGLPAETNCISTLSSYAPKSIIPWVGMDAPGTFSCPVSVVDFLNIKSFSLIFRYDPLV
                     MTYISNTPNPVFNATNGYLTVTDASAAGGLRRITMTFNGLNTISLPDNSILCGFQFNY
                     IGGEAAIGWRTNSSGCTYYGPGGILKCDQPYSNYFIDGGIVSMLAPVTKVDSAALSPG
                     EFGTFTVRVWDFQDIRAGILTLGYNPAALIYQETIPNPALGEEFEANLVNPGLLQFSW
                     SEAGITLADASIIVHLTFQYLGGNSTLYWINDGTSCQYFHSNIPSPMTDIPTSSYYFN
                     GNVENAVYIWSGDNSSEWGSDGNWENGEAPNQNMDVIVDPTVNPDNWPVFDGDFTLGE
                     HCRNLIINGNAQLIINGNLNVLPGRALSLTGNGILKVDGNWTNSGIFYPGDGTIEFTG
                     TTNAAIAAGDPPGNYVSNYSLTPFSSTFIPLSGGLPGPAGNDSHSDVPIGFPFTYLGV
                     SYSMARINTNGWLSLNLSGEDTGSGNN"
     gene            complement(2342..3622)
                     /gene="glyA"
                     /locus_tag="PKZ74_03550"
     CDS             complement(2342..3622)
                     /gene="glyA"
                     /locus_tag="PKZ74_03550"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPM91861.1"
                     /translation="MKRDYQVFRILGKEKKRQLHGIELIASENFVSNQVLRAMGSVMT
                     NKYAEGYPGKRYYGGCQFVDETEQLAIDRAKELFNCEYANVQPHSGAQANMAVFLAFL
                     KPGDKFMGLDLSHGGHLSHGSPVNSSGILYQPIAYGVDKETGRVDYDKMEEIALREQP
                     KLIIAGASAYSRDWDYARMRAIADKAGAILMADISHPAGLIARGLLNDPMPYCHVVTT
                     TTHKTLRGPRGGLILMGKDSENPWGWATPKGEIKMMSQLLNSAVFPGIQGGPLEHVIA
                     SKAVAFGEALTDEYFEYILQVRKNASVMAKAFVSKGYHVISGGTDNHLMLIDLRNKFP
                     ELTGRLVENTLVRADITVNKNMVPFDSRSPFQTSGLRIGTPAVTTRGLKEKEMPVIVD
                     MIDEVLHNIENETILLSVKKRVNEMMEKYPLFSA"
     gene            3752..5818
                     /locus_tag="PKZ74_03555"
     CDS             3752..5818
                     /locus_tag="PKZ74_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM91862.1"
                     /translation="MRKPAFIMIFLILVMLPAALTGQAVNLKCLEVLPGGDVQVRWQP
                     LQLGASFSNYTIYSSLNRMGPYSVAGVINVISADSYIHSGASAQHDTVHYYLVTNQGP
                     GQSTFTDTLSTILLTGSTIDFESISLQWSPLHSPEPFLPEMHPWYLIYREFPAGSWLL
                     TDSTQNLIRDHHFWECNSANDSVRFRIGLRNDVFGCVSLSNQVSFILKNLSNRYPPVI
                     DSVSIDAGGNAVIGWQPGTEPDIRGYKIFLVTSSNDSIDYVDGRFSTSYSDQQSDPCD
                     GPVKYILLSIDSCGNESPFPFDPNPPYPDKPHSTIYLQDIQYDPCNMTNRLQWNEYEN
                     FTPPLAITRIYVSTAGGPFLELASVLPGQTGFVHAGLEPNTEYAYFVRAFSGDMAKSS
                     SSCIKQARSYNSPYPLFMYLRYATVGPEGQVEVLFYTDTNAHVQSYRILRSDSPGGPF
                     VEAGTVEDVGEDMPGFTDPLADANTGSYYYQVEVIDSCGVASIIANMARTIFLRVEAL
                     PELVNRLEWNAYESWTGGVAGYRIYRRLDQLALEQIGEVGPGQLTYYDNVSAFTGNYS
                     RISYVVVAYEGAGNAFGFQDESYSNEAVAEQEPRVFIPNVIMPNGANNVLKPVTVFVG
                     AEGYEFMVYNRWGEQVFYTAEPGLGWDGRYKGKLVPQDVYVYLIRFLDALGQPRQVKG
                     NVVVIY"
     gene            5867..7339
                     /gene="guaB"
                     /locus_tag="PKZ74_03560"
     CDS             5867..7339
                     /gene="guaB"
                     /locus_tag="PKZ74_03560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPM91863.1"
                     /translation="MTQDNQKFLGEALTYDDVLLVPAYSEVMPREVEINTFFSRNIHL
                     NIPVVSAAMDTVTESMMAIAIAQEGGIGVIHKNMTIEDQANQVRKVKRAENGMILDPV
                     TLPVNALVRDAFMLMKEYGIGGIPVVQGNKELVGIVTHRDLRFERNLERPIAEVMTKD
                     NLITTNEFVSFEKAAEILQKHRIEKLPVVNDHNRLVGLITYKDIIKIQAHPNAYKDSQ
                     GRLRVAAAVGTAPDTLDRAAALVEANVDAIVVDTAHGHTRGVIAIAKRIKKEFPGIDL
                     VVGNIATASAALALAEAGADAVKVGIGPGSICTTRVIAGVGVPQLSAIYDVSRALKGT
                     GIPVIADGGIRYSGDIAKALAAGADTIMAGSLFAGVDESPGETIIYDGRKYKAYRGMG
                     SIEAMQKGSKDRYFQDVEDDIRKLVPEGIVGRVPFKGTLSEVITQLVGGLRAGMGYVG
                     ARNLEMLKQAKFIKITHAGVVESHPHDVSITSEAPNYSRI"
     gene            7382..9376
                     /locus_tag="PKZ74_03565"
     CDS             7382..9376
                     /locus_tag="PKZ74_03565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM91864.1"
                     /translation="MRKIRFIATMSVLLVFMTGGLHAQKTDNRILLNIAGEEVTAGDF
                     MYVYKKNNINREDQGLDSISKYLQLYINFRLKVREAEELGLDTAKAFITELEGYRKQL
                     AQPYFYDSTISEKLIREAYERKQWDLRASHILFTCDKNALPQDTLKAFQSAMAARNRV
                     MAGEDFSKVSVELSQDPNARDSEASQYRPARKGNLGDLGYFTVFDMVYPFESAAYNTP
                     VGTVSMPVRSDHGYHVIKVTDRKPALGQVQVAHIFVSLAAGSTTQDSIQKKIKIDAAY
                     EKIMAGESFEDIVKEFSEDKGSAANGGKLPWFGSNRLVPDFVDAARNLKDSGDISKPF
                     STIYGYHIIKLLGKKPVGTFEEEEFGLKTRIEKDMRNKLSEEAVISRIKQENNFREYP
                     KAVEAVYATLDSSVMKWEWTAAKAAGLKKPVFSIGKLKYSQQDLATYLASKQTKSVTS
                     IRGFFNDTYKSFVKEKCMAYEDSRLEQKYPDFRLLIREYHDGILLFDLMERNVWSKAV
                     KDTAGLAAYYGANKDKYMWDERFHATVVTVLNPGDVNIEELRDFITEGKPLEEILERY
                     NSDTTLNILAETEKYSRGDSPIIDKVQWKTGLSPMIDSPSGPAFVYGIAVAPPEPKLL
                     QEARGLVTADYQSFLEEKWIGELRSKYPVTINQEVLSTLK"
     gene            9387..10226
                     /locus_tag="PKZ74_03570"
     CDS             9387..10226
                     /locus_tag="PKZ74_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91865.1"
                     /translation="MRKIVLLILPALVACTGLFRADEDDRVVARANDKELRISEVAEK
                     IPDGLPSRDSITLAENYISKWIQNEILTRKAKETLKSNEQDFDKQLEEYRNSLLLFSL
                     EQKLVSQFLDTVITQQEIEAYYIENRSQFELKGNIVKFDFVKINKRSRHAREFRNLLK
                     ADKDESMMRLADYAEKNATDYWFAGEWVSFNDLLDEMPLEVDNQELFLRRNSYAEATD
                     SLFIYFVRINDFKTSDSISPLEFEKDKIRNIILNARKIDLIERKRQEFIDQAFKEDKA
                     KIF"
     gene            10284..11678
                     /locus_tag="PKZ74_03575"
     CDS             10284..11678
                     /locus_tag="PKZ74_03575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM91866.1"
                     /translation="MKARRLYAFRGILFLGMILTGLPMTGIGQDEIVVDRIVAIVGKN
                     MILESDLESQYLQLRMQGEIEGSASSVKCQILQKMLRDNLLLNQAELDSVEVSDSDVE
                     QNLDRRLRYFISQFGSQEKLEQYYEKSLLEIKEEFRQMVKDFMLIEQVQNDITKNVFV
                     TPNEVKAFYGTIPSDSLPLVSARVEMTQIIKMPPVSAEQKVMIKERLRDLRRRVMAGE
                     NFATLAILYSEDPASAAKGGEIGFFGRGELYPEYEAAAFKLKEGEISDIIETKAGFHI
                     IQLMERKGDYINTRHILLQTKPSPEDLEKARLELDSLLLQIRNGEITFDDAVVKFSDD
                     PGKVSGGFIINDYDGTTWFPMDQLDPQVSFTVNKLEVGEISSPVPTQTEEGKEAFRLI
                     KLKSRSEPHRANLVDDYSMITNWALENKKEQKIVEWVNKNVDNAYIMIIDDYTDCDFE
                     NKWFPGKKQENENR"
     gene            11708..12676
                     /locus_tag="PKZ74_03580"
     CDS             11708..12676
                     /locus_tag="PKZ74_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM91867.1"
                     /translation="MISYKSDVEAVDAFVEKYGALRAEIAKVIVGQDEVVKRVLISIF
                     SRGHGLLVGVPGLAKTLMVNTIARALGLSYNRIQFTPDLMPSDIIGTEILDEERKFKF
                     IHGPVFANIILADEINRTPPKTQSALLEAMQEKAITVAGKNYKLPEPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIWLDYPTFEQEIEVVRSTTQDIPVDLKVILSAEEILFFQG
                     LIRRIPVPDNVYKYAVSLSAKTRPGTPAAHPLANDFVIWGAGPRASQYLIIGAKCHAA
                     IQGKYSPDIEDVRAVASSILRHRIVRNYKAEAEGYPIEKIMMEFLK"
     gene            12701..14083
                     /locus_tag="PKZ74_03585"
     CDS             12701..14083
                     /locus_tag="PKZ74_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPM91868.1"
                     /translation="MKKSKVYLISLAVLLLIVIIWIATGSGPEKETTLEVPVKSGLFD
                     ITVTTTGELEAKSSEKIMGPVNLRDFRIWQIKIEDIIPDGTVVDSGQYIATLDRTELV
                     NKMKDQELEIEKLETQFTKTQLDTTLELRSARDNLINLKYALEEKQIVVDQSIYEPPA
                     TQRQAKIDLERAMRNYEQAVENYEVKLRKAEADMQEINTSMKKVRSEYAKMVSLLNEF
                     TIFAPKSGMVIYKRGWDGQKQGVGATISVWEPVVAELPNLREMKSKTYVNEIDISKVK
                     VNQAVRIGVDAFPDKKFTGVVREVANIGQQLPNSNAKVFEVSIEVNEFDSILRPAMTT
                     KNEILTSSLDSVLFIPLDCVQSNDSISYVYTGGNRRQVVLGLSNENEVIVRAGLKAGE
                     EVYLVPPSDAEKWKLTKLDEATLEKIRQEDEAKARQQVPAGLPDSMMMPPGNGKFRMQ
                     KGGGKPPFKR"
     gene            14096..>15322
                     /locus_tag="PKZ74_03590"
     CDS             14096..>15322
                     /locus_tag="PKZ74_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91869.1"
                     /translation="MKKPELQSKFYERYVHSLNIAVEAVIANRFRSMLTALGIIFGVA
                     AVIAMMAIGNGARKEILDQMKMVGVNNIVITPLLEKSGGEGNSDDEGGGQKLQKKYSP
                     GLTLEDAESIRQIIPAVTRVSPEVKYDSYVIKEGKRSPAKLIGVTNDFFEVYNLQLYA
                     GQYFQEQQQVNGSPVCIIGPSVKARFFSREDPLGKTIKCGGIWLTVIGVLENRAMQVD
                     ETKDLGISDYNDHIYAPIQTVMLRYKDRSYVNDASLRQGESSVMFIDGGIAAFSSSSS
                     GSMDNNYNQLDKIVVQVTDSKYLDATTKALTSMLKRRHQQVDDFQVSVPELLLKQEQR
                     TKDIFNIVLGVIAGISLLVGGIGIMNIMLASVMERTKEIGIRRATGATKRDVIFQFLS
                     EATIISISGGVIGIVLG"
BASE COUNT         4073 a         3664 c         4004 g         3581 t
ORIGIN      
        1 gattatttcc cgagccggta tcttctccgc tgaggttaag cgatagccag ccattggtat
       61 ttatcctggc catagagtaa ctgactccca ggtaagtaaa cgggaagccg atgggaacat
      121 ccgaatggga atcgtttccg gcaggccccg gcagtcctcc gctcaagggt ataaaggttg
      181 aagagaacgg ggtaagtgag taattggaaa catagtttcc cggtggatcg cccgcagcaa
      241 ttgcggcatt ggttgttccg gtaaattcaa tggtcccgtc gcccgggtaa aaaatcccgg
      301 aattggtcca gttgccgtca acctttaaga tgccgtttcc cgtcagactt agggcacgac
      361 ccggcagtac attcaggttc ccgttgatga tcagctgtgc attgccgtta atgatcaggt
      421 tccggcaatg ttctccgagt gtaaaatcgc catcgaatac gggccagtta tccggattga
      481 cagtcggatc gacgattaca tccatgttct ggttcggggc ttcaccgttt tcccagttcc
      541 cgtccgaacc ccattcgctt gaattatcac ccgaccagat atatacagca ttttcgacgt
      601 taccgttaaa gtaataactg gatgtcggaa tatcggtcat aggtgagggt atattactat
      661 ggaaatactg gcaggaggtg ccgtcattaa tccagtacag tgtcgaattt ccgccaaggt
      721 attgaaaggt gaggtgtaca ataatgctgg catcggcaag agtaatgcct gcttcagacc
      781 agctgaactg gagcagcccg gggttgacca ggttggcttc aaattcctcc ccgagggcgg
      841 gattgggtat ggtttcctgg tatatcaggg ctgccgggtt gtaccccaga gtgagaatac
      901 cagcacgtat atcctgaaaa tcccatactc tgacggtaaa agtgccgaat tcgccgggcg
      961 acagtgccgc cgaatcaact ttggtcaccg gggccagcat cgatactata cccccgtcga
     1021 tgaaataatt ggaatagggt tgatcgcatt tcaaaatccc acccgggcca taataggtgc
     1081 aaccgctgga gttggtccgc cagccaatag ccgcttcacc accgatataa ttgaactgaa
     1141 atccgcaaag gatcgagtta tcaggaagcg aaatggtgtt caaaccatta aatgtcatcg
     1201 taattcttct caggccacct gcagccgagg catcggtaac ggtcaggtat ccgtttgttg
     1261 cattgaagac cggattgggc gtattgctga tataggtcat taccaggggg tcataccgga
     1321 agatcagtga gaaagatttg atgttcagaa aatctactac gctgacaggg caggagaatg
     1381 tccccggggc atccatgccc acccagggta tgatcgactt cggggcgtat gaactgagtg
     1441 tggaaatgca attggtttca gcgggtaatc cgtagggtaa tcccggaatg taatcgtatt
     1501 gccagttgaa ctggttgacg tgttccgttg ccggagaagt gatattggga tagatcttta
     1561 ccagtgtgat gcaaacccct cctccgccca tcatgcctcc cagggaaggg tcttcctgga
     1621 gatcccagat cacagcaggg cagaaagttg aatcctggaa tacttccgga tcatcgacat
     1681 gaaaattcac cgcaaagatt tttgtccatc cgttggtcgg caagtaagtg accggtgtgc
     1741 tggatacaac tttgacggcc ccgttcacat attcgtaatt gccttctatg ccgaataact
     1801 ccatgcttaa tcccgtgctg tcggtggtta tttccggggg attgggaatc gtaagcccgt
     1861 atgcggcaat gatttcagtg aaagatgtga actccaggac atcgtcgtca tagaaaaaac
     1921 ggatattcat tccgtgcagc cgtttattgg ccgagtcaca ttgcatctct acatcgagtg
     1981 aataagtctg ggtagcgcat acatattccg ggttattaaa ccgaaccgta acgacagggt
     2041 actgtgaaaa cgccgctgaa gagccaagca gaataataac tatggccagc acggagacac
     2101 ttttagggat aaaagatggt gtgatcatgt ctcttgattt tcggtaacaa atataaatac
     2161 atacattaat ccttcaatcc attcaaggtt atattttcag gatagcaatt gacttaattt
     2221 gttgcaattt agaatgattc ctgataatgc ggtttcattc cggttaagtc ctggctaagc
     2281 tgaagattcc aggaaggatt ttgaaaagat tatactgaaa atcagagagt aagtttcagt
     2341 ttcaggcaga aaataacgga tatttttcca tcatttcatt gacccgtttt ttgacggaga
     2401 gcaggatcgt ttcattttcg atgttgtgga gtacttcatc gatcatatcc actatcaccg
     2461 gcatctcttt ttccttcagt ccgcgggtgg ttacagccgg cgtcccgatc cgcagcccgg
     2521 aggtctggaa cggggaacgg ctgtcgaacg gaaccatgtt tttgttgacg gtaatatccg
     2581 cccttaccag tgtattttcg accagccggc cggtgagttc ggggaattta ttccgcagat
     2641 cgatcagcat caggtgattg tcggttcctc cggaaataac atggtagcct ttgctgacaa
     2701 aggcttttgc catgacggag gcgtttttcc gcacctgcag gatatattcg aaatattcat
     2761 cggtgagggc ttcgccgaag gcaacggctt tggaagcgat gacatgttcc agcgggccgc
     2821 cctggattcc cgggaaaacg gcagaattca gcagttgcga catcattttg atctcccctt
     2881 tcggagtcgc ccatccccaa ggattctcgc tgtctttgcc catcaggatc agcccgccgc
     2941 gtggtccgcg aagggttttg tgggttgtgg tggtaacgac atggcaatac ggcatcggat
     3001 cgttcagtaa tcccctggcg atcaagccgg ccgggtgcga aatatcggcc atcaggatgg
     3061 cgccggcctt gtcggctatc gcccgcatcc tggcatagtc ccagtccctg ctataagccg
     3121 atgcccctgc aatgatcagc ttcggttgct cccgcagggc aatttcttcc atcttgtcat
     3181 aatccactct gccggtctct ttatccacac cgtaagcgat cggctgatag aggataccgg
     3241 aactgttcac cggtgatccg tgagagagat gcccgccatg cgacaggtca agccccatga
     3301 atttatcgcc gggtttcagg aaggccagga aaaccgccat attggcctgg gctccggaat
     3361 ggggctgcac attggcgtat tcgcaattga acagctcttt ggcacggtct atcgccagtt
     3421 gttccgtttc gtccacaaac tggcacccgc cataataacg cttgcccgga tagccttccg
     3481 catatttatt ggtcatcacc gatcccatgg cccgcaagac ctggttgctg acaaaattct
     3541 ccgatgcgat cagctcaatc ccgtgaagct gccttttctt ttccttaccc aggatacgaa
     3601 atacctgata atcccgtttc atatacacat tgtttgttat ggttaatttt caaaaaacgg
     3661 ttacaaaggt aatggaaata tgaagatcgt taatgggatt ccgtttacct gcccctgatt
     3721 tttgactaat tttgcaagac atttcaaccg gatgcgcaag cctgctttca tcatgatttt
     3781 ccttatcctc gttatgcttc cggctgctct caccgggcag gcagtgaatc tgaaatgcct
     3841 ggaggtgctg cccggaggtg atgtgcaggt acgctggcaa ccgctgcaac tgggggcctc
     3901 attcagcaac tatacgatat attcttcctt gaacaggatg ggtccatatt ccgttgccgg
     3961 agtgatcaat gttatttcag ccgatagcta tatacactcc ggagcctctg cccagcacga
     4021 tacggtacat tattacctgg tcacgaacca gggtccggga cagtcgacat tcaccgatac
     4081 gctgtcgacc atactgctga ccggttcaac tattgatttt gagagtatca gcttacaatg
     4141 gtcgcctctg cacagccctg agccgtttct tccggaaatg catccctggt atttaattta
     4201 cagggaattt ccggcaggat cctggctgct caccgattcc acccaaaacc tgatacgcga
     4261 tcaccatttc tgggagtgca acagtgccaa cgattcagtg cgattcagga taggcctgag
     4321 aaacgatgtt ttcggctgcg tttccctttc aaaccaggta tccttcatcc tgaaaaacct
     4381 ttccaacagg tatccgcccg taattgattc cgtgagtatt gatgcaggcg gaaacgctgt
     4441 gatcgggtgg cagcccggta ctgaaccgga tatccgggga tataagattt tccttgtgac
     4501 atcatccaac gactccatcg attacgtcga tggcaggttt tccacatctt attcggatca
     4561 gcaatccgat ccctgcgacg gcccggtcaa atacatcctt ctgtctatcg attcatgcgg
     4621 aaatgaaagc ccatttcctt tcgatcccaa tcccccgtat cctgacaaac cgcattcgac
     4681 catttacctg caggacattc aatacgatcc gtgtaatatg acaaacaggc tgcaatggaa
     4741 tgaatatgag aactttacac caccgctggc gataacgagg atctatgtca gtaccgccgg
     4801 cggccctttc cttgagctgg catcagttct gcccggccag acgggttttg tacatgccgg
     4861 cctggaaccc aacaccgaat atgcctattt tgtcagggcg ttcagtggcg atatggccaa
     4921 gagctcttct tcctgcatca aacaggcccg gtcgtataat tctccttatc cgcttttcat
     4981 gtacctgagg tatgccacgg tcgggccgga agggcaggtc gaagtgcttt tttacaccga
     5041 tacgaacgcc catgttcagt cgtaccgcat tctgcgcagc gacagccccg gcgggccgtt
     5101 tgttgaagcg ggcactgttg aggatgtggg agaggatatg cccggcttca ccgatccgct
     5161 ggccgatgcc aatacaggga gctattatta ccaggttgaa gtgatcgata gctgcggagt
     5221 ggcctccatc attgccaaca tggcccggac catctttctg cgtgtggagg ctttgccgga
     5281 gctggtgaac cggctggaat ggaatgcata tgaatcctgg acgggaggtg tggccggata
     5341 ccggatctac cgcaggctcg accagctcgc gctggaacag atcggagagg tcgggccggg
     5401 ccagttaaca tactacgaca atgtgtcggc tttcacagga aactacagca ggatatccta
     5461 tgtggtggtt gcttatgaag gggcaggcaa tgcatttggc tttcaggatg aaagttattc
     5521 caatgaagcg gtggcagagc aggaaccccg tgtctttatt cctaatgtga tcatgccaaa
     5581 cggggccaac aatgtgctca agcctgtcac ggtgttcgtc ggcgcggaag gttacgaatt
     5641 catggtctat aaccggtggg gcgaacaggt gttttatact gccgaacctg gtttgggatg
     5701 ggatggacga tataaaggga aactggttcc ccaggatgta tatgtttacc ttatccggtt
     5761 cctcgatgca ctgggccagc ccaggcaggt caaaggcaac gtggttgtga tttattgagt
     5821 tcattaaggg ttttttacta catttgcaaa ctaaattttt ctggatatga cgcaggataa
     5881 ccaaaaattc ctcggcgaag ccttaacata tgacgacgtc ctcctggtgc cggcctattc
     5941 ggaagtgatg ccacgggaag ttgagatcaa cacctttttt tcgagaaata ttcacctgaa
     6001 tatcccggtt gtatcggcgg cgatggacac ggtgaccgag tcgatgatgg ctattgccat
     6061 tgcgcaggag ggtggaatcg gtgttatcca taaaaatatg accattgaag accaggccaa
     6121 ccaggtgagg aaggtcaaac gtgccgaaaa cgggatgatc ctcgacccgg tgaccttgcc
     6181 ggtcaatgcg ctggttcgcg atgccttcat gttgatgaaa gaatacggta tcggcggcat
     6241 tccggtagtg caaggcaaca aggaactggt cgggatcgtc actcatcgtg atttgcggtt
     6301 cgagcgcaat ctcgaacggc ccattgccga agtgatgacc aaagacaatc tcatcactac
     6361 caatgagttc gtctccttcg agaaagcagc agagatcctt caaaaacacc ggatcgagaa
     6421 gcttccggtg gtaaatgatc acaaccgcct ggttgggctt atcacgtata aagatatcat
     6481 taagatacag gcacatccga atgcatacaa agacagccag ggtcggttac gtgtagcggc
     6541 tgcggtaggg accgcacccg atacactcga cagggctgcc gctttggtgg aagccaatgt
     6601 cgacgcgatc gtggttgata cggcccatgg gcatacccgg ggcgttatcg ctatcgctaa
     6661 acggattaaa aaggaatttc cgggcatcga tctcgtcgtc ggaaatattg ctacggcttc
     6721 ggccgcgctg gcgctggcag aagcgggcgc cgatgcggtc aaggttggga tcgggccggg
     6781 atccatttgc acaacccgtg ttattgcggg ggttggcgta ccgcagcttt cggccattta
     6841 cgatgtaagc cgggcactga aagggaccgg tattccagtc attgccgatg gagggatccg
     6901 gtattcgggc gacattgcca aagcattggc ggccggcgcc gataccatca tggccggttc
     6961 cttgtttgcc ggcgtggatg agtcgccggg tgaaacgatt atctatgacg gaagaaaata
     7021 caaagcctat cgtggaatgg gctccatcga agccatgcag aaaggatcga aggaccgcta
     7081 tttccaggat gttgaggatg acatccggaa actggtaccg gaagggattg tcggccgggt
     7141 gccctttaaa ggcacactgt cggaagtcat tacccagctg gtaggcggac tcagagccgg
     7201 gatgggatac gtaggggccc gaaacctgga gatgctcaag caggcaaaat tcatcaaaat
     7261 aacgcacgcc ggagtcgtcg aaagccatcc gcacgacgta tccattacca gcgaggcacc
     7321 gaattacagc aggatttaat cgcagacata tttataaaat aacagaaaat cagcttacac
     7381 catgagaaaa attaggttta ttgcaaccat gtcagtcctt ctcgttttta tgaccggcgg
     7441 gctccatgcg caaaaaacgg ataacaggat actgttgaat attgcaggcg aagaggtgac
     7501 agccggcgat ttcatgtacg tttataaaaa aaataatatc aaccgggaag accagggatt
     7561 ggacagtatc tccaaatacc tgcagcttta tatcaatttc aggttgaaag tcagggaagc
     7621 ggaagagctg gggctcgata cggccaaagc ttttattacc gaactggaag gataccggaa
     7681 acaattggcc cagccctatt tttatgattc caccatcagc gagaaactga tcagggaggc
     7741 ttatgagcgc aaacaatggg atctccgcgc cagccatata ttgtttacgt gtgataaaaa
     7801 tgcactgcct caagatacat tgaaagcctt ccagagtgcg atggctgccc gcaacagggt
     7861 gatggccggc gaggattttt caaaggtgtc ggtagaactt tcacaggatc cgaacgcaag
     7921 ggattctgaa gccagccagt accgcccggc ccgcaaaggc aacctgggcg acctgggcta
     7981 ttttacggtt ttcgatatgg tttacccgtt cgaatcggcg gcctacaaca cccccgtcgg
     8041 aacggtctct atgccggtcc gcagcgatca tggttaccat gtgatcaagg taaccgaccg
     8101 aaaaccagca ctgggccagg tacaggtggc ccatatcttc gtcagcctgg cagccggttc
     8161 gacaacccag gattccatcc agaaaaagat taagatcgat gctgcttacg aaaaaatcat
     8221 ggccggtgaa agttttgagg atatcgtcaa ggaattttcg gaagataaag gatcggcagc
     8281 aaacggggga aaactcccct ggttcggatc aaaccggctg gtccccgatt ttgtcgatgc
     8341 ggccagaaac ctgaaagatt caggcgatat cagcaaaccc ttcagtacca tttacggcta
     8401 tcatatcatt aaactattgg gtaagaagcc ggtaggtact tttgaggaag aggagttcgg
     8461 ccttaaaacg cgtatagaaa aagacatgcg caataagctc agtgaagagg ccgtaatcag
     8521 tcgtatcaag caggagaata acttcaggga atatcccaaa gcggttgaag ccgtttatgc
     8581 aaccctcgat tcaagtgtga tgaaatggga atggacggcc gctaaagcag ccggcttgaa
     8641 gaaaccggtg ttttccatcg gcaaactcaa gtattcccag caggatctgg caacttacct
     8701 tgcttccaaa caaaccaaga gcgttaccag catccgggga ttcttcaatg acacatataa
     8761 aagcttcgtg aaagaaaaat gcatggcata tgaagacagc cgtcttgagc aaaagtatcc
     8821 cgatttcagg cttttgatcc gtgaatacca cgacggtata ctgcttttcg acctgatgga
     8881 gagaaatgtc tggtcgaaag cggtgaaaga tactgccggg ctggctgcct attatggcgc
     8941 caataaagat aagtacatgt gggatgagcg tttccatgcc accgtcgtca ctgttttgaa
     9001 ccccggggat gtcaatatcg aagagctgag agatttcatc accgagggga aaccactgga
     9061 agagatcctt gaacgttaca attcggatac tacgctgaat atcctggctg aaacagaaaa
     9121 atattcgcgc ggagattccc cgattatcga taaagttcag tggaagaccg gcctgtcgcc
     9181 tatgattgat tcgccttcgg gtccggcttt tgtatacggc attgccgtag cgcccccgga
     9241 accgaagctt ttgcaggagg ccaggggact ggtcactgcc gattaccagt cgttcctcga
     9301 ggagaaatgg attggcgaat tgcgcagtaa gtacccggtc accatcaacc aggaggtcct
     9361 ttcaactttg aaataaacag cacgccgtga gaaaaattgt cctgctcata ttaccggctc
     9421 ttgttgcctg caccggcctt ttcagggccg atgaagacga ccgggtcgta gccagggcaa
     9481 atgacaaaga actcaggatt tcagaagtgg cagagaagat acccgacggc ttgccttcca
     9541 gggatagtat aacacttgca gaaaactata tctccaaatg gatccagaac gaaatcctga
     9601 cccggaaagc caaggaaacc ctgaaatcga acgaacagga ttttgacaaa cagctggagg
     9661 aatacaggaa ttcgcttctg cttttcagcc tggagcaaaa actggtcagc cagtttctcg
     9721 atacggtgat cacccaacag gaaattgagg cctattacat cgaaaaccgc agccagtttg
     9781 aattgaaagg aaacattgtg aagttcgatt ttgtcaaaat caataagcga tcgaggcatg
     9841 cacgggagtt tcggaacctg ctcaaagcgg ataaagatga aagtatgatg aggcttgctg
     9901 attatgccga aaagaatgcc accgattact ggtttgcagg agaatgggtt tcatttaacg
     9961 acttgcttga tgaaatgccg ctggaagtgg ataaccagga actgttcctc cgccgcaaca
    10021 gttatgcaga agcaaccgat tcccttttta tttatttcgt caggataaac gatttcaaga
    10081 catccgacag tatctccccc cttgaatttg aaaaggataa gatcaggaat attatcctga
    10141 atgcaagaaa aattgatctt atcgaacgaa aaaggcagga gtttatcgat caggccttta
    10201 aagaggataa ggccaagata ttttaatcta tctgaaaaca ataaaacaat cattccgtgg
    10261 ttaaatctct atcagatgct attatgaaag ccaggcgcct atatgctttt cggggaatat
    10321 tgtttttggg aatgatcctg accggtcttc cgatgaccgg aatcgggcag gatgaaattg
    10381 tggtcgaccg gattgtggcc attgtcggca agaatatgat cctggaatcg gatcttgaat
    10441 cgcaatatct tcaactcagg atgcagggcg aaatcgaggg atcggcttct tcggtcaaat
    10501 gccagatctt gcagaagatg ctgagagata atttgttgct gaaccaggcc gaactcgaca
    10561 gcgttgaagt atccgacagc gatgtggagc agaacctcga ccgcaggttg cgctatttta
    10621 tctcccagtt cggttcacag gaaaaactgg agcagtatta cgaaaagtct ctcctggaaa
    10681 ttaaggagga attccggcag atggtgaagg atttcatgct gatcgagcag gttcagaatg
    10741 atatcacaaa gaatgtcttt gtcacaccca atgaagtcaa agcattttat ggcaccatac
    10801 cgtccgacag cctgccattg gtcagcgccc gtgtcgagat gacccagatc atcaagatgc
    10861 cgccggtaag cgccgaacag aaggtgatga tcaaagagcg gctccgcgac ctcaggcgca
    10921 gggtcatggc cggggaaaac tttgccacgc tggccatcct ctattcggaa gaccccgcct
    10981 cggctgccaa aggaggtgag atcggattct ttggaagggg agaactctac ccggaatacg
    11041 aagcggctgc atttaagctg aaagaaggtg aaatatcgga tattattgaa accaaagccg
    11101 ggttccatat catccagtta atggaaagaa aaggggatta tatcaatacc cgccatattc
    11161 ttctccaaac aaaaccctca cccgaagacc ttgaaaaggc caggcttgag ctcgacagcc
    11221 tcctgcttca aatccgcaac ggggagatca cattcgacga cgctgtcgtt aaattctccg
    11281 atgacccggg caaggtcagc ggaggattca tcattaatga ttatgacggc acgacctggt
    11341 ttccgatgga ccaactcgat ccccaggtct cattcacggt gaataagctg gaggtcgggg
    11401 aaatctcttc accggtaccc acccaaacgg aggagggaaa agaggctttc cggcttatca
    11461 agctaaaatc caggtcggag ccccaccgcg ctaacctggt ggatgattac agcatgatca
    11521 ccaactgggc gctcgaaaat aaaaaagaac aaaaaatcgt ggaatgggtt aataagaatg
    11581 tcgacaacgc ttatatcatg ataatcgatg attacacgga ctgcgatttt gaaaataaat
    11641 ggtttcctgg taaaaaacag gagaatgaaa acaggtaagt gattttcaat cccggaaaaa
    11701 taatacgatg atatcttata aatctgatgt tgaagctgtt gatgctttcg tagagaaata
    11761 tggcgccctt cgggcggaga ttgccaaagt gatagtgggc caggatgagg tcgtcaaacg
    11821 ggtgcttatt tcgatattca gccgtggaca tggattgctg gttggtgtcc cgggcctggc
    11881 aaaaacactg atggtcaata ctatagcaag ggctttggga ctaagctata accgcattca
    11941 gtttacaccc gacctgatgc catcggatat catcgggacc gagatcctgg acgaggagcg
    12001 gaagtttaaa ttcattcacg ggccggtttt tgccaatatc atcctggctg acgagatcaa
    12061 caggacgccc cccaaaacac aatcggctct gcttgaagcg atgcaggaga aggccattac
    12121 tgttgccgga aagaactata aactaccgga accatttttt gtactggcaa cccagaaccc
    12181 gatcgagcag gaagggacct atccgctgcc ggaagcgcaa ctcgaccggt ttatgttcaa
    12241 tatctggcta gattatccaa catttgagca ggagatcgaa gtggtcagga gtaccacaca
    12301 ggatatccct gtcgacctta aagtgatcct cagcgccgag gaaatactct ttttccaggg
    12361 cctgatccgc cgaatcccgg tacccgacaa tgtttacaag tacgctgttt ccttgtcggc
    12421 aaagacgcgc cccggaacgc ccgccgcaca tcctcttgca aacgacttcg ttatatgggg
    12481 agctggtcct cgcgcatcac agtacctcat catcggggcc aaatgtcatg ccgccatcca
    12541 gggaaaatat tcgcccgaca ttgaagacgt cagggcggtg gcttcctcca tcctgcgaca
    12601 ccgcatcgtt agaaattata aagccgaagc ggaaggatat ccgatcgaaa agattatgat
    12661 ggaatttttg aaataaagcc taatcctaac accggctgta atgaaaaaaa gcaaagttta
    12721 cctcatcagc ctggccgttc tcctgttgat tgtcatcatc tggatcgcca ccggcagcgg
    12781 cccggaaaaa gaaaccaccc tggaagtgcc cgtcaaatca ggcctctttg atattacggt
    12841 cacaactacc ggcgagctcg aagccaagag ctccgaaaag atcatggggc cggtcaacct
    12901 gcgcgatttt cgaatctggc agataaaaat cgaagacatc attcccgacg gaacggtggt
    12961 cgattcgggt cagtacattg ccacactcga ccggacagaa ctggtcaata agatgaaaga
    13021 ccaggagctg gagatcgaaa agctcgaaac acagttcacc aaaacgcaac tcgacacaac
    13081 gcttgaactt cgttcggcac gggataacct catcaatctc aaatatgccc tggaagaaaa
    13141 gcagatcgtt gtcgaccagt cgatttatga gccgccggct acgcagaggc aggcaaagat
    13201 cgacctggaa agggcaatgc gcaattatga gcaggcggtg gagaattacg aagtgaagct
    13261 gcgcaaggcg gaagccgata tgcaggagat caatacttca atgaaaaagg tccggtcgga
    13321 gtacgctaaa atggtgagcc tgctgaacga gttcaccatc ttcgccccga aatcgggcat
    13381 ggtgatatac aagaggggat gggacggaca gaaacaaggc gtcggcgcaa cgatcagcgt
    13441 atgggaaccg gttgtggccg aattacctaa cctgagggag atgaagtcga aaacttatgt
    13501 taatgagatc gatatcagca aagtaaaggt aaaccaggcg gtcaggatag gggtcgatgc
    13561 ctttcccgac aagaaattta ccggggtcgt ccgcgaagtg gccaatattg gccagcagct
    13621 gcccaattcc aatgctaaag tgttcgaggt cagcattgag gtcaatgagt tcgattcgat
    13681 cctcaggccg gccatgacga ccaagaatga aattctgaca tcgagcctgg acagcgttct
    13741 gttcataccg ctcgattgtg tccagtcgaa cgacagtatc agctatgtct ataccggagg
    13801 aaaccgaagg caggtagtcc tggggctgtc aaacgagaac gaggtgatcg ttcgtgccgg
    13861 actcaaagca ggcgaagagg tttacctggt tcctccttca gatgcggaga aatggaagct
    13921 gacaaaactg gatgaagcga cactggagaa gatccggcag gaggatgagg caaaagccag
    13981 gcagcaggtg ccggcaggcc ttcccgattc gatgatgatg cctcccggca acggaaaatt
    14041 caggatgcag aaaggcggcg gcaagccccc cttcaagcgg taagctttga taagaatgaa
    14101 aaagcccgaa ctccaaagca agttctatga gcgttatgtt cattcgctga acatagcggt
    14161 ggaggccgtg attgccaacc ggttccggtc aatgctgacc gccctgggca ttattttcgg
    14221 cgtagccgcc gtcatcgcca tgatggccat aggcaacggt gcccggaagg aaatcctcga
    14281 ccagatgaaa atggtgggcg tcaataatat cgttatcacg ccattgctcg aaaaatcagg
    14341 gggcgaggga aattctgacg atgaaggcgg agggcagaaa cttcagaaga agtattctcc
    14401 cggtctgacc cttgaagatg ctgaaagcat ccggcagatc atccctgcgg taacgcgggt
    14461 gagccctgag gtgaagtacg acagctatgt gatcaaggaa ggaaaacggt cgccggccaa
    14521 attgattggt gtcaccaacg attttttcga ggtttacaac ttgcagttat acgccggaca
    14581 gtatttccag gagcagcagc aggtcaacgg aagtcctgtt tgtatcatcg ggcccagtgt
    14641 gaaagcccgg ttctttagca gggaagaccc cctgggtaag acgataaaat gcgggggaat
    14701 ctggctgacg gtgattggag ttctggaaaa ccgggcaatg caggtcgatg aaacaaaaga
    14761 cctggggatc agcgattaca acgaccatat ttatgcaccc atccagacag tcatgctccg
    14821 gtataaagac cgttcctacg tgaacgatgc atcgctccgg cagggtgaaa gttcggtcat
    14881 gttcatcgat ggcgggatcg ctgcattttc aagcagcagc tcgggttcca tggacaataa
    14941 ttataaccag ttggacaaga tcgtggtcca ggtgaccgac agcaaatacc tcgacgctac
    15001 aaccaaagct ctgaccagta tgctgaaaag acgtcaccag caggtggatg atttccaggt
    15061 cagtgtcccg gaactattac taaagcaaga gcaaaggacc aaagacattt tcaacattgt
    15121 gctgggggtt attgcgggca tttccctgct ggtcggcggg atcgggatca tgaatattat
    15181 gctggcctcg gtgatggagc gtaccaagga gatcgggatc cgaagggcca ccggggccac
    15241 aaaacgcgat gtcattttcc agttcctttc cgaagccacc attatctcta tctccggagg
    15301 agtcatcggt attgtgctgg gt
//
LOCUS       DAOPNH010000041        15063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3956_length_15063_cov_6.638193, whole genome shotgun sequence.
ACCESSION   DAOPNH010000041 DAOPNH010000000
VERSION     DAOPNH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_15063_cov_6.638193"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1025..2497)
                     /locus_tag="PKZ74_03595"
     CDS             complement(1025..2497)
                     /locus_tag="PKZ74_03595"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPM91870.1"
                     /translation="MRRRRAIRVILYILAAFVLIVAGLFAYYKLATKVHEPEIDPSAL
                     ASYERTISGSSVNSCEHGWLKKERDGWWVMYLEGSPYEVGYAHGLLTQELMAAQEKAF
                     LERLAELIPSKFYQRFMLGFVRFFNRNLDQYVKEEYRQEIYGISRFASPEFGFMGPAY
                     ARMLNYHAAHDLGHAVQNMNLVACTAFGVWDGRTPDSNLVIGRNFDFYVNDDFAKDKI
                     LCFIRPDSGYGFVMITWAGFAGVVSGMNEKGLTVTLNAAKSSIPGGAKTPVSLLAREI
                     LQYAANIDEAGRIAASRETFVAESFLVASAADHKAVVIEKTPEDLAIYDPGADFIILT
                     NHFQSDKFLNDQLNVENMKNEVTVYRYDRVKELLEENPVITPEIAANILRDYRGLDGA
                     DIGMGEEKAINQFIAHHSVIFEPGKLRIWVSGQPWQLGEYWMYALDSLRNSAKPDESF
                     TTGVHRVNTEGHGGGSTVIPADTLLSSDVFREFLMKREEQ"
     gene            complement(2490..3980)
                     /locus_tag="PKZ74_03600"
     CDS             complement(2490..3980)
                     /locus_tag="PKZ74_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM91871.1"
                     /translation="MNYDFVITGSGLGGLVSGIILAKEGHSVCILEKNHKPGGNLQTF
                     ERFGHSFNTGLHYIGSMEEGQTLHRFFNYLGIAGKLPLVKMDEDGYDRIVFGEEETVY
                     RHAQGWERFEEEMERQFPSERDSISTYVKSIRDIIRSIPLYDLENASTPRLEMSQLNR
                     CTMDFLRGITRNRRLISVLAGANSIYHGAAGRTPLYLHACIRNSFVSSAWRIKGGSDK
                     LTDLLAETFTSLGGKILTGAEVTGFIPQNDRIASVELRDGRRIEGKQFISNLHPGATL
                     KLTGLETLRKSYRHRIENLENTAGFFSLYLVFRENTFPYLNHNLFQYYHDDYFKDDLP
                     EEKWPHTYMIYTPYHDDNKGFTKTASILTFMKNEQYRQWENLAAAGRGEDYRGFREMA
                     SQKLLDAVEKRYPGIRSSVARFCDATPLTFADYTGTPGGSGYGILKDCHDPVRSIITP
                     KTKIPNLFFTGQNLNIHGVLGTTISAIVTCSEFTGFRNLVNKIVHA"
     gene            complement(4004..7909)
                     /locus_tag="PKZ74_03605"
     CDS             complement(4004..7909)
                     /locus_tag="PKZ74_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM91872.1"
                     /translation="MSLIFTRIFDFFARYRLVFFLFLGAALAVIIWLLLQLRIVEDIS
                     KVLPHDEKVDQYLEVVNHSAAAEELVFFVGPADASQTIPPEELIRFAGDLADSIRGSL
                     MPGLVAEIRDRSDDLAFKGIYTELHDNLPVFLTEEDYARLDSLTSREAVRNAVKAAYK
                     NLVSPAGMVTRDMVLADPIGLTALALEKRGAPGIDESYRIYDGYVFSSDLRYLIFFIK
                     PAQKPTETSKNTALIKRLDEITGNLSRENNGEIKAGYFGTAAVSVANARQLKRDIILT
                     MNLAIVLLVLFIGFYFGRFRVIPAIVFTTLMSAGISAAVLALIGREISGVSLGFGAVL
                     LGIAIAYFIHYLNHLRDVREPRKVLKEIALPIVMSSIISSGDFFTLLLVRSEAVRDLG
                     LFAGFSILAAGLMTLVFLPHLTKKINWDKSRENLVNRSVRKIASYPVEKKKLVPVMIL
                     LLTVLFYFTSKRVTFEGDLTGMSYMPPELAASESTLDRISQYSLRSVFVVTKGENIDQ
                     ALEQTGRFVQKAERLREEGIVKKYTAVTGLVVPLAEQQRRLERWKAYWTEEKKAEVKG
                     TLTEAATAAGFRAGSFGRFFDWLEKDHKTVGRDDLPELSKIFIDPYISVNDKMATVTT
                     ILKVRQEDKGRLYEALQNETSSFIFDKQYLTSTFMDILNSDFNKLVIISLLIVFVVLL
                     LSYGRIELALITFIPMFMSWIWTLGIMGLFGMKLNIFNMIITSFVFGLGIDYAIFSIQ
                     GMLQQYKYGHGDVTAFRSSVLMDAITTLVGLGVLVLAVHPALKAMAFAAIIGIISVWF
                     VTWSMEPVLFNWLVYQNDRKRPRPVTLKDFIFSISSLSIFIIGSMFIMLYGLLFIKFL
                     RFRTRWAKYALHYQMSAFSKVLIYSNILSSLKINKPEEEDFRKPAMVIANHQSHNDIA
                     LLLILHPKFLEMTNDRVQRNKFFGPLIKMADFYAISDGMESLLPRLRKNVEEGYSVVI
                     FPEGTRSPDGRIQRFHKGAFYLAEELNIDLLPIIIHGSGDIYSKGEYLLKTGRAVIEV
                     LPRITPAEARSKGNLLERSRAIRHYMTAEYRKVAGKAETPRYFRERLVKNYLFKGPVL
                     EWYLKIKVRLEKNYALFHKYLPVDGHITDIGCGYGFMIYMMNFLSDKRTFTGIDYDAE
                     KIEVAKNCPAMNRRIEFIAGDALTMELPKSRAFVMADVLHYFPESEQERLIIRCIENL
                     EDSGLLIIRDGDRKMGKKHIGTRLSEFFSTNLGFNKTRGSAKQLYFTSREKIVEVLMR
                     NGMAVEVIDETKVTSNIVIIARCPHPVPPPAGGGSKGGG"
     gene            complement(7934..9142)
                     /locus_tag="PKZ74_03610"
     CDS             complement(7934..9142)
                     /locus_tag="PKZ74_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPM91873.1"
                     /translation="MPEFTSYPGFNSQGCCVIIPTYNNEKTLEGVISGVLEFTHDIIV
                     VNDGSTDSTREICAGFDDITVIHLERNRGKGYAIRKGFREALRLGFRYAITIDSDGQH
                     LPEELPKFIEKITAEPDSLIIGARNMDQEGVPGGSSFGNRFSNFWTWVETGYRLPDTQ
                     SGYRLYPVRKMAKMRFITNRFEFEIEVLIRSAWKGIPLTPVPVSVVYHPEKERVSHFR
                     PFTDFARISLLNSVLTILALLFFRPWMMIRKMSKEGFRELVRKHLLSPEESNFRKSAS
                     IALGIFMGIAPVWGWQMMIALALSIALKLNKAITLIASNISIPPMIPFILFGSYITGG
                     LVLGNHQPLDFGPEITLDFVKKNLIQYVIGALVFGAIMGILAGFFTYLLLTVFRRRQK
                     TVAEGTGPGK"
     gene            complement(9170..9640)
                     /locus_tag="PKZ74_03615"
     CDS             complement(9170..9640)
                     /locus_tag="PKZ74_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HPM91874.1"
                     /translation="MRKTGWIIIGAVAAAFIIIQFFRPEKNDKVTNPKNDIVFSFNMP
                     VNVKKKIVNACYDCHSETTAYPFYNRVAPVSWIIAGHIREGKKHLNFSDWASYDRKDQ
                     IKLLNEICETITNGEMPLKGYVMMHSKAVFNEREIEEICAWTEQAAEELLSRKD"
     gene            complement(9637..10038)
                     /locus_tag="PKZ74_03620"
     CDS             complement(9637..10038)
                     /locus_tag="PKZ74_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HPM91875.1"
                     /translation="MKKLVLLSIVLVGAAIALMSFTFSKVNTSNPPQEQAQAMFPAEI
                     QKIFETSCFDCHSDTGNAKAKLKLNFSKWGELSDAKKVGKIGNINDEVQSGKMPTARY
                     ISNYPDRALTKEQKELVNKWATEESAKLMGQ"
     gene            complement(10074..10433)
                     /locus_tag="PKZ74_03625"
     CDS             complement(10074..10433)
                     /locus_tag="PKZ74_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91876.1"
                     /translation="MIKPYTLSILPNDGQGFTASVTFDPSHLIFAGHFPGQPVVPGVV
                     LVEIATAFISQLTGKNLVVKEASVIKFLKVVDPRMNWVLLIDCSIVEEGEDGYKANLR
                     IYSGEVDFVKFKGIWFQ"
     gene            10960..11850
                     /locus_tag="PKZ74_03630"
     CDS             10960..11850
                     /locus_tag="PKZ74_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91877.1"
                     /translation="MKTKIFFLSLFLHLICFYGNTQAYNPLVLENAQWKVIQDDDATP
                     WIDSQGGWLIRGDTLIDGVAYKKLFERAFEEPYSNVITNQGLYGFLREDTLNKTVYII
                     ESPPGFYGCDSADQEYLLFDFSYEVGDTSQMCLHTENILSFVIEITQEYILGANRKVF
                     QFYNSSSFIEGIGHESGLLESPVISLSGGPYYYLEEYCLGTDEECNVVYVKIDEKEQV
                     SHYRIYPNPCRGYFYIKGMTDINENIHYSVNDVIGKEVVSGETPANSEFQIYLKEPGL
                     YFLHIRRQGETPSCYKVVNL"
     gene            12313..13164
                     /locus_tag="PKZ74_03635"
     CDS             12313..13164
                     /locus_tag="PKZ74_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91878.1"
                     /translation="MKKLSKLITAIKVLVVFIAVFQTGKALPQQQWKFHLAFEDANYQ
                     RDTIWFIWDSTATFHGIDTALGEMAVELNYSEFNVWIQNFYGDTTKTQALPYNHTHST
                     TIGAINFELPIIMTWDSSMFFADYLPPEPVGWVNSAKLSNDYFFFINNDHLNHVFDMT
                     LTDSIIAPEPNNTDPWYWLPERHFPMGIELRQDPTIGLDENLCEMKSNIVLFPNPSST
                     SIFLRLKDYSYPLKVSIFSLTGKLEMSQEIHNNDQFIDISGLSSGMFILLITNSNQKI
                     YEKFIKN"
     gene            13309..14211
                     /locus_tag="PKZ74_03640"
     CDS             13309..14211
                     /locus_tag="PKZ74_03640"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91879.1"
                     /translation="MRKFFILLMLLPATATFSQEFAPVGAIWHYTQSNPFQGDGWTFE
                     TMESVIDTSIENVKCKKIINIIRNDADTFIQVNKYFYKNNDSVFFYEDGSFNLLYDFG
                     AETGDTITLSYFPTYDGSPLKMIIDSVGIIIINGNDRKIQYITCGDGILVEFGNEIIE
                     DIGSTHNMFPVYDGTLNGPLRCYEDSITGQYLSGFHPDYGWNFKDCAEIITRINEVKD
                     YDGIFLYPNPASSSITVKNVDLPKNFKIINIFGQVMNEGQLMTSRTIYLTGVANGVYY
                     LILHEGSYQEEYQQDNRYKFIICH"
     gene            14226..>15063
                     /locus_tag="PKZ74_03645"
     CDS             14226..>15063
                     /locus_tag="PKZ74_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91880.1"
                     /translation="MYIQIKLRNLVVVLIMITSHICSSQDPFNYGQICNSEPESSPIE
                     NAPFYVCSRTTPFWSTSNRLHFTPSDSNIYIAHLNIIIVQRNGEDPGNWSDNELFRDI
                     ISRAIDTLNYYYSNLCIVPIIEYPNCFDTTLGEPDYIPSINIQFTYSIYFIANENYWG
                     SVNPDTSWSSYLNSHDPWRRGINIYYTMDSTTYQLLVEENQLDPNMISYNWVNSMPSE
                     TNMERKQYMVLPNKYAEFVYILNNYIPDTAQVISNTVHGYARIWKHELGHIFGLNHSS
                     IPD"
BASE COUNT         3961 a         3528 c         3515 g         4059 t
ORIGIN      
        1 caacatatag cccttccagg ttgtttgcca catcggcata ggtgtaattg atcaacgctt
       61 ccgtatacct gacagccgta tcaatcggat aattgctgcc ggatttcaat ccttttccca
      121 aagattccct gtaagaaatg atttgttgta ccatattttg agaagaggga tccatttgct
      181 gggttaaatt ggtttgcgga tccgaacgat cggaattctt tttacagctt aaaattacaa
      241 caattcagag aatagtgaat gcaactaaca agactaattt tttcatttgg tttgtttttt
      301 aaattatttt ttcagttata ctcattcttt gcaggtttgc gaaagcaaaa ctgcttagaa
      361 tgagttatac cgataggaac agcagcataa aattcgctaa tactgaaaaa tcggttaagg
      421 ctctgttccg gctcggtata attctcacat cagaaaagaa aagctatgta gggtaaagca
      481 atcccaggta atgaatcaga tgatattatc cggcaagaaa ataacatggc taagaataga
      541 aataagatcc aattggttaa agaagaattc tcgaaatgga tgaaacaaat ataattaata
      601 caaatacgaa agtcaagggc ggttaataac tttctggttt gagacgccat ctggtttggt
      661 tgagatggca tctcatcttg cgagactcca tctcaaattc gagatggcat ctcataaacg
      721 gagactccat ctcatttcag gcagatggag tctcgttaaa ttcgagatgg agtctcaaag
      781 aattacccgg ttcggcattt ggcatttaaa atttaatatc gccggatcgt ctgttcggga
      841 tttcaaccga tgaattaatc ccgccggctc gtcaatcttg aaactgcacc tttttttcca
      901 gggactggcg tccctgcaac catactgtcc tccctacaag agaagaacat ggatttttta
      961 ttttattgcc caactgtcca actgcgatcc ttcgtccgcc aaaggcggac tcaggaaatg
     1021 ctgcctactg ttcttccctc ttcatcagaa attcccggaa aacatcggag ctcagcaagg
     1081 tgtcggcagg aattacggta ctgccccctc cgtgcccctc cgtgttcact ctgtgtaccc
     1141 ctgtggtgaa agattcatca ggtttcgcag agttacgcaa agagtccagc gcgtacatcc
     1201 agtattcccc caactgccac ggctgaccgg aaacccagat gcgcagcttc ccgggctcga
     1261 aaatgaccga atgatgtgcg atgaactggt tgatggcttt ctcttcgccc atcccgatat
     1321 cggcgccatc cagccctctg taatcgcgca ggatgttggc cgcaatctcc ggtgtgataa
     1381 caggattctc ttccagaagc tctttcaccc ggtcgtaacg gtataccgtg acttcattct
     1441 tcatgttttc cacgttcaat tggtcgttga ggaatttatc cgactggaaa tggttggtga
     1501 ggatgatgaa atcggcgccg ggatcgtaaa tggccagatc ttccggcgtc ttctcgatga
     1561 caacggcctt gtggtccgcc gccgacgcaa ccaggaacga ctcggctacg aaagtctccc
     1621 ggcttgcggc gatgcgaccg gcttcgtcga tgttcgcagc atactgcagg atctccctcg
     1681 ccagcagcga caccggcgtt ttcgccccac ccggaatgct ggatttggcg gcattgagcg
     1741 tcaccgtcag ccctttctcg ttcatccccg acaccacgcc ggcaaaaccg gcccaggtga
     1801 tcatgacaaa gccatacccg ctgtcgggcc tgataaaaca caggatcttg tctttagcaa
     1861 aatcatcatt cacatagaaa tcgaaattcc gcccgatgac caggttgctg tcgggcgtcc
     1921 ggccatccca tacgccgaaa gcggtgcagg ccacgaggtt catattctgc acggcatggc
     1981 ccaggtcgtg cgcggcatga tagttcagca tccgggcata cgccggcccc atgaaaccga
     2041 actcgggcga ggcaaagcga gaaatcccgt aaatctcctg ccggtattcc tctttcacat
     2101 actggtcgag gttccggttg aaaaagcgga cgaatcccag catgaaccgc tggtagaact
     2161 tcgacgggat cagctccgcc agccgctcca ggaacgcttt ctcctgcgcg gccatcagct
     2221 cctgtgtcag caatccgtga gcatagccca cttcgtacgg actcccctca aggtacatca
     2281 cccaccagcc gtcacgctcc ttttttaacc atccgtgttc gcagctattg acagaggacc
     2341 ccgatatggt cctttcgtaa gaggccaacg ccgatgggtc gatttcaggc tcgtggacct
     2401 tcgtggccag cttgtaatag gcgaacaggc cggcgacgat cagcacgaat gccgccagga
     2461 tgtaaagaat gacccgtatg gctctccttc tacgcatgga cgatcttgtt gacgaggttg
     2521 cggaaaccgg taaattcgga acaggtgacg atggccgaaa tagtcgtccc cagcaccccg
     2581 tgaatgttca ggttctgccc ggtgaaaaag aggttgggaa tctttgtttt cggcgtaatg
     2641 atcgaccgga cagggtcgtg gcaatccttc aggatgccgt aaccgctgcc accgggcgta
     2701 ccggtgtaat cagcaaatgt caacggcgta gcgtcgcaaa atcttgcgac gctactacgg
     2761 attcccggat atcttttttc aacggcatcc agcagttttt gagatgccat ctcacgaaac
     2821 cccctgtaat cctcgccacg gcctgctgca gcaaggtttt cccattgcct gtactgttca
     2881 tttttcatga aagtaaggat acttgccgtc ttcgtaaatc ctttattatc atcatgataa
     2941 ggcgtataga tcatatatgt gtgcggccac ttttcttccg gaaggtcatc cttaaaataa
     3001 tcatcatgat aatactgaaa gagattgtga ttgaggtagg gaaaggtatt ctccctgaag
     3061 accaggtaaa ggctgaagaa gccggccgtg ttctccaggt tctcgatccg gtggcggtaa
     3121 ctcttgcgaa gggtttccaa ccctgttagc ttcaaagtgg ctccggggtg gaggttcgag
     3181 atgaactgct tcccttcgat ccggcgtccg tctctaagtt ctaccgaggc tatccggtca
     3241 ttctggggga taaaaccggt gacttctgcc ccggtgagga ttttcccgcc aagagaggtg
     3301 aaagtttctg caagcaggtc ggtcagcttg tcgctcccgc ctttgatccg ccaggcgctg
     3361 ctcacgaacg aattccggat gcaggcgtgg aggtagagcg gcgtacggcc cgccgcaccg
     3421 tggtaaatac tgttggcgcc agccaggacg ctgatcagcc gccggttccg ggtgatgcct
     3481 ctcaggaagt ccatcgtgca acggttgagc tgcgacatct caagccgggg tgtactggca
     3541 ttttccaggt cgtacaacgg gatgctgcgg atgatatccc gaatcgattt aacataagtt
     3601 gagatggagt ctcgttccga aggaaactgc cgctccatct cctcctcaaa ccgttcccac
     3661 ccctgcgcat ggcggtaaac cgtctcctcc tccccgaaaa cgatccggtc gtagccatcc
     3721 tcatccatct ttaccagcgg cagtttgccg gctatcccga ggtagttgaa gaaacggtgc
     3781 agcgtctggc cctcttccat gctgccgatg taatgcaaac ccgtgttgaa agaatgcccg
     3841 aaacgttcga acgtttgaag gttgcctccc ggcttatggt tcttttccag gatgcacaca
     3901 gaatgcccct ccttcgccag gatgatcccg ctgaccaggc cgccgaggcc gctacccgtt
     3961 atgacgaagt cataattcaa agttccaagt tcagattcca gattcagccc cctcccttac
     4021 tccctccccc agccggggga gggacagggt gggggcagcg agcaataatg acaatattcg
     4081 acgtcacctt cgtttcatca ataacttcaa ccgccatacc attccgcatc aacacctcca
     4141 cgatcttctc ccggctggtg aaataaagct gctttgcact gccccgggtc ttgttgaagc
     4201 ccaggttggt gctgaaaaac tccgaaagcc tcgtcccgat atgctttttc cccatcttcc
     4261 ggtcgccgtc gcggatgatc agcaggccac tgtcttccag gttctcaata caacggataa
     4321 ttagccgttc ctgttcactc tccgggaaat aatgcagcac atcggccatc acaaaagccc
     4381 tgcttttcgg gagttccatc gtcagggcat cgccggcgat gaattcgatc cggcggttca
     4441 tggcggggca gttcttcgcc acttcgattt tctccgcatc ataatcgatc ccggtgaatg
     4501 tcctcttatc cgaaaggaaa ttcatcatat agatcatgaa accgtacccg caaccaatat
     4561 cggtaatgtg cccatccaca ggaagatatt tgtgaaatag tgcatagttc ttttccaatc
     4621 ttacctttat cttcaggtac cactccagca ccggcccttt gaaaagataa tttttaacaa
     4681 gcctttcacg gaaatagcgg ggcgtctccg ccttcccggc aaccttcctg tattccgctg
     4741 tcatataatg cctaatcgcc cgtgaccgtt cgagcagatt cccttttgat cgggcttcag
     4801 cgggggtgat acgcggcagc acctcaatga ccgcacggcc cgtcttcagg aggtattccc
     4861 ctttggaata gatatcgccc gatccgtgaa taataatggg aagaaggtcg atattcagct
     4921 cttcggcaag gtaaaaggct cctttgtgga aacgctggat gcggccatcg ggcgatcgcg
     4981 tcccttccgg gaagatcacc accgagtagc cctcttccac gttctttctg agcctgggca
     5041 aaagcgactc catcccgtcg gaaatggcat aaaaatcggc cattttgatc aggggcccga
     5101 agaacttgtt gcgctgaacg cgatcgttgg tcatctccag gaacttcgga tgaaggatca
     5161 gcagcagtgc tatgtcgttg tgcgactgat ggtttgcaat aaccatggcg ggtttccgga
     5221 aatcctcctc ttccggtttg ttgatcttta aagacgatag gatgtttgaa taaatgagaa
     5281 cctttgaaaa ggccgacatc tggtagtgca gggcatactt cgcccatcgg gtcctgaacc
     5341 tcaggaattt gatgaaaagt aaaccgtaga gcatgatgaa catgctcccg ataatgaaaa
     5401 tactcagcga agaaatagag aaaataaaat ctttcagggt gaccggccgg ggccgtttgc
     5461 ggtcgttctg gtaaacgagc cagttgaaca gcaccggttc catgctccag gtcacgaacc
     5521 agactgaaat gatccctatg attgccgcga aggccatagc tttcagcgcg ggatgcaccg
     5581 ccaggaccag cacccccagg ccgaccaggg tggtgatcgc gtccatgagc accgacgacc
     5641 ggaaggccgt cacgtcgccg tggccgtatt tgtactgctg cagcatcccc tggatcgaaa
     5701 agatggcgta gtcgatcccc agcccgaaca caaaggaggt gatgatcatg ttaaagatgt
     5761 tcagtttcat cccgaaaagg cccataatac ccagcgtcca gatccagctc atgaacatcg
     5821 ggataaacgt gatcagggcc agctcgatgc ggccatagga gaggagcaaa accacaaaaa
     5881 caataagtaa agatatgata accagcttgt tgaagtcgga gttaagaata tccatgaaag
     5941 tgctggtcag gtattgttta tcgaagatga acgaagatgt ttcattctgc agcgcttcgt
     6001 aaagtcgtcc tttgtcttcc tgtctgacct tcaggatcgt cgtaaccgtc gccatcttat
     6061 cgtttaccga aatataagga tcaatgaaaa ttttactcag ttcaggcaga tcatcccgtc
     6121 ctacagtctt atggtccttc tccagccagt cgaagaatct gccgaagctg ccggccctga
     6181 agcctgccgc cgtcgccgct tcggtcaacg ttcccttcac ctctgccttt ttctcttccg
     6241 tccagtaagc tttccaccgt tccaggcgcc gttgctgctc tgccaatggt accaccagtc
     6301 cggttacggc agtgtatttt ttgacgatgc cttcttccct gagccgttcc gccttctgca
     6361 caaagcggcc ggtttgctcc agcgcctggt cgatattttc tcccttcgtc acaacaaaaa
     6421 ccgaccgcag cgaatactgg ctgatcctgt cgagggtcga ttccgatgcg gctaattccg
     6481 gcggcatata gctcatcccg gtaaggtcgc cttcaaaagt cacccgcttt gaggtgaaat
     6541 aaaagagaac cgtcagcagc aggatcatca ccggtaccag tttcttcttt tcgacagggt
     6601 aggaggcgat ttttcgtacc gaccggttga caaggttttc gcggcttttg tcccagttta
     6661 ttttttttgt aagatgggga aggaagacca gggtcatcaa tcctgcggcc aggatgctga
     6721 aaccggcgaa cagtcccagg tcgcggacag cttcggagcg gaccagcagc agggtgaaga
     6781 agtcgcccga ggagatgatg ctgctcatga caatcggcag ggcaatctct ttcagtactt
     6841 tacgtggttc ccttacgtcg cgcagatggt tcaggtaatg gatgaaatag gcgatggcga
     6901 ttcccagcag caccgccccg aatcccagcg aaacacccga gatctcgcgc ccgatgaggg
     6961 ccaggacggc agccgatatc cccgcgctca taagggtggt gaagacgatg gccgggatga
     7021 cccggaaccg gccgaaatag aaaccgatga acagtaccag caggacgatg gccaggttca
     7081 ttgtcaggat aatatcgcgc ttgagctgcc gggcattggc gacagagacg gcggcggtgc
     7141 caaaataccc tgccttgatc tcaccgttat tttcccggct caaattaccg gtaatttcat
     7201 ccagtctttt gatgagggcg gtgtttttag aggtctccgt cggcttttgg gccggtttta
     7261 tgaagaagat gagatacctg agatcggaag agaatacgta gccgtcatag atccggtagc
     7321 tctcatcgat accgggtgca cctctttttt ccagcgccag ggctgtcagc ccgatgggat
     7381 cggcgaggac catgtcgcgg gtgaccatgc cggcagggga gaccaggttc ttgtaagccg
     7441 ccttcacagc attccgcacc gcctcgcgcg aggtcagtga atcgagccgg gcataatctt
     7501 cttccgtaag gaaaacaggc aggttatcgt gcagttcagt gtaaatgcct ttaaaggcaa
     7561 gatcgtcgct acggtcacgg atttccgcta ccaggcccgg catcaatgaa ccgcggatgc
     7621 tgtcggccag atcgccggca aaacggatca gttcctccgg cgggatagtc tgcgaagcgt
     7681 cggccgggcc gacgaaaaac accagctctt ccgccgcggc cgaatggttc acgacttcca
     7741 ggtattgatc cactttttcg tcgtggggga gcactttgga gatgtcttcg acgatccgga
     7801 gctgtaagag gagccagatg atgaccgcga gcgctgcccc caggaaaagg aagaagacca
     7861 gcctgtagcg ggcgaaaaaa tcgaatatcc tggtaaagat caggctcatt ctcgtctaag
     7921 agttaataaa aaatcacttc cccggtccgg tcccttcagc aaccgtcttt tgcctgcgcc
     7981 tgaaaacagt cagcaggagg taagtgaaaa agcctgctaa aatacccata attgcaccga
     8041 aaaccagcgc cccgataaca tattggataa gatttttttt gacgaaatcc agggtgatct
     8101 ccgggccgaa atcgagcggc tgatggttgc caaggaccag tccgccggtg atgtagctgc
     8161 cgaacaggat gaacgggatc atgggcggga tgctgatatt ggaggcgatc agggtgatgg
     8221 ctttgttcag tttcagggcg atggaaaggg ccagggcgat catcatctgc cagccccaca
     8281 ccggcgctat gcccatgaat atgcccaggg cgatggaggc cgatttccgg aagttcgact
     8341 cctccgggct gagcaggtgc ttcctgacca gttcccggaa cccctctttg ctcatcttgc
     8401 ggatcatcat ccaggggcgg aaaaacagca gggccaggat agtcaggacc gaattgagca
     8461 ggctgatacg ggcaaaatcg gtgaaggggc ggaagtgtga aactctttct ttttcaggat
     8521 gataaaccac gctgacgggc accggggtca gcgggatccc tttccaggcc gaccggatca
     8581 gtacttcgat ctcgaattcg aaccggttgg tgatgaaccg catctttgcc atctttcgga
     8641 cagggtagag gcggtagccc gactgcgtgt cgggcagcct gtaacctgtt tccacccagg
     8701 tccagaagtt ggagaacctg tttccgaacg aggacccgcc cggcacccct tcctggtcca
     8761 tgtttcgcgc gccgatgatc agggaatcgg gttcggcagt gatcttttcg ataaatttcg
     8821 ggagctcttc cgggaggtgc tgcccgtcgg aatcgatggt gatggcatac cggaacccaa
     8881 gccggagcgc ttcgcggaac ccttttctga tggcatagcc cttgccccgg tttcgctcca
     8941 ggtggatgac cgttatgtcg tcgaatccgg cgcagatttc ccgtgtcgaa tcggtcgagc
     9001 cgtcgtttac cacaataata tcatgggtaa actccagtac gcctgaaatc acaccttcca
     9061 gcgttttctc gttgttgtag gtagggataa tgacgcagca gccctgagag ttaaagccgg
     9121 ggtatgaagt aaattcaggc atggtcagga aatccggtgc aatgccgttt tagtccttcc
     9181 gggataaaag ttcttcagcc gcctgttcgg tccaggcaca gatctcttcg atctcgcgtt
     9241 cgttgaagac cgccttgctg tgcatcataa cgtagccttt cagcggcatc tcgccgttgg
     9301 tgatggtttc acagatctca ttcagcagtt tgatctggtc tttgcggtca tagctggccc
     9361 agtcggagaa gttcaggtgt tttttccctt cccggatgtg gccggcgatg atccatgaaa
     9421 caggcgccac ccggttgtaa aacggatagg ccgtcgtctc ggagtgacaa tcatagcagg
     9481 cattgacgat cttcttcttg acgttgaccg gcatgttgaa cgagaaaacg atatcgtttt
     9541 tcggattggt taccttgtcg ttcttttccg gccggaaaaa ctggataatg atgaaagcgg
     9601 ctgcgaccgc cccgatgatg atccacccgg tctttctcat tgccccatca gtttggccga
     9661 ttcttccgtg gcccatttat tcaccagctc tttctgctct ttggtgaggg cgcggtcggg
     9721 atagttagaa atgtagcgcg cagtcggcat tttaccgctt tgtacttcgt cgttgatgtt
     9781 cccgatcttt cccacttttt tggcatccga cagctcgccc catttggaga agttcagctt
     9841 cagcttggct ttggcattgc cggtatcgga atgacagtcg aaacaggagg tctcgaagat
     9901 tttctgtatt tcagccggga acatggcctg ggcctgctcc tggggcggat tggaggtgtt
     9961 tacctttgaa aatgtgaatg acatcagcgc aatagccgcg ccgacaagta cgattgataa
    10021 taagaccagc tttttcatag tatggatttt tttgatgatg acaaaaataa gcattattga
    10081 aaccaaatcc ccttgaattt tacgaaatcg acctcacccg aatagatcct caggttagct
    10141 ttgtacccgt cctcaccctc ctcaacaata gaacaatcga tcaatagaac ccaattcatc
    10201 ctcggatcaa cgactttcag gaacttgatg accgatgctt cttttacgac caggttcttc
    10261 cctgtaagct gcgaaatgaa agctgtggcg atttcgacca gtaccacgcc cggcaccacc
    10321 ggctgcccgg ggaagtggcc ggcgaagatg aggtgggagg gatcgaaggt gacggaggca
    10381 gtgaacccct ggccgtcgtt cgggaggatt gatagggtgt agggttttat catagggtgg
    10441 ttataaggtg gtcataaagt tgttaaaagg tgtttataag gtgcacttgc aaaattcaat
    10501 caaaaattgc aaccgagcat gctgaatctg attaaaaatt gcaaccgagc atgctaaatc
    10561 tgattaaaga atgcaaccag gatactgaat ttgtatcaac ttattttttc agacaattta
    10621 aatccgcgtt aatccgtatt attcgcgtca tccgcgtgct attgacagaa cacggataaa
    10681 catgaatgca acggatctac acggattatt gatttttgct gtgaaacatc ttgccttttt
    10741 cgatatcatg ccttaccttt gaaccgcctt gaaatttgaa ttgatttttt ctcaccgggg
    10801 ctttaagccg caatcgaccc ggaaggcgta agtgatatta agaatttgtc attaatatac
    10861 tgactttcat gggcattgaa taaaaacaaa tgttttgcac tgcgtttaaa cgatgataat
    10921 ctatatttat gaatatgtaa actattttaa ctattagcta tgaaaacaaa aatcttcttc
    10981 ttaagcctgt tcttacacct gatttgcttc tacggaaaca cacaagccta taatccgctg
    11041 gttcttgaaa atgcacagtg gaaagtcatc caggatgatg atgccacacc ctggatagat
    11101 agtcagggag gctggttgat tcgcggtgat actttgattg atggagtggc gtacaaaaaa
    11161 ctcttcgaga gagctttcga agaaccttac tcaaatgtca ttaccaatca agggctatat
    11221 ggatttctgc gtgaagatac tttgaacaaa actgtttata taattgaatc gcctccggga
    11281 ttttatggtt gtgactccgc agatcaggag tatctattgt ttgatttttc ttatgaggtg
    11341 ggtgatactt ctcaaatgtg tcttcatacg gaaaatattt tatcatttgt gatagaaatt
    11401 acacaagaat atatactcgg agccaataga aaagtatttc aattttataa tagctcatca
    11461 ttcatagaag ggattggtca cgaatccggc ttgctcgaat cacctgttat aagcttgagc
    11521 ggaggtccat attattacct tgaagaatat tgtctcggta ctgatgagga atgcaacgtg
    11581 gtttacgtaa agatcgatga aaaggagcaa gtatcacatt atcggattta cccaaatcct
    11641 tgtagaggtt atttttacat aaaaggcatg acagatataa atgagaatat tcactattca
    11701 gttaatgacg tgatagggaa agaggttgtt tcaggggaaa ctcctgcaaa cagtgaattt
    11761 caaatctatt tgaaggaacc gggcttgtac ttcctgcata tcaggcggca gggtgaaacc
    11821 ccgtcttgtt ataaagtggt taatctttaa ctcctatctt atttctttag gcaattttaa
    11881 atccgtgtta atccgcgtgc tattgacaga acacggatac cacgcctcgc ctgcctgacg
    11941 gcagtcgggc aagggatgca acgaatttcc gcggattatt gatttttgcc gaaaaatatc
    12001 ttgactttat cgatataata acttatcttt gacccgccag gtaaatttga attgatttta
    12061 tctcaccggg gctttaagcc gcaatcgacc cggaaggtgt aagtgatatt aagaaattgt
    12121 cattaatatg ctgactttca tggacgttga acaaaaacaa atattttgca ctgcgtttaa
    12181 acgatgataa tctattttca ttagatgtaa tcgatattga tcataaggct gataaacaga
    12241 tagatataaa ctatttattt aaattatgga tttgaagttt tttttaaaaa tactttctaa
    12301 aacgatttaa ttatgaagaa attgagcaag cttattactg caataaaagt cttagtagtg
    12361 tttattgctg tgtttcaaac cggaaaagca ttaccacaac aacaatggaa atttcatctg
    12421 gcctttgagg atgctaacta ccagcgagat acaatttggt ttatttggga ttcaaccgct
    12481 acatttcatg gtatagatac ggctttaggc gaaatggcag tggaactgaa ctacagtgaa
    12541 ttcaatgttt ggatacaaaa tttttacgga gatacaacta aaactcaagc tcttccctat
    12601 aatcataccc attcgacaac tattggagct ataaattttg aattaccaat tatcatgaca
    12661 tgggattctt caatgttctt tgctgattat ttaccaccgg aaccggtagg ttgggtcaat
    12721 agtgctaaac tgtcaaatga ttatttcttt tttattaata atgatcacct taatcatgta
    12781 tttgatatga ccttgacgga ttccattatt gcgcctgaac caaataatac tgatccttgg
    12841 tattggttac cagaaaggca ttttcctatg ggtattgaac tcaggcagga tccaacaata
    12901 ggacttgatg agaatttatg tgagatgaaa tcaaatattg tcctatttcc aaatccatcc
    12961 agtacatcca tctttcttag actgaaagat tattcatatc ctctaaaagt atccatcttt
    13021 tctttaacgg ggaaattaga aatgagtcag gaaattcata ataatgacca attcattgac
    13081 atctccggct tatcatccgg gatgtttatc ttattaataa ccaatagtaa tcaaaagatt
    13141 tatgaaaaat ttattaaaaa ttagtcttat tgctatgctt ttaagtgtag taagatttaa
    13201 agtattccaa acagtacttt cgggctggta ttttataagg atttcggatt cgggaaaacc
    13261 cattccagta aattctgttg tcaatctttg ttctaaatat taaatcgaat gagaaagttc
    13321 tttatactcc ttatgttatt gccggcaaca gcaacattct cacaagaatt tgctccggtg
    13381 ggtgcaatat ggcattatac acaatccaat ccttttcaag gtgatggttg gacctttgaa
    13441 acaatggaat cggttattga tacttccatt gagaatgtta agtgcaaaaa aatcattaat
    13501 ataattcgaa acgatgcgga tactttcata caagtgaaca agtattttta taaaaataat
    13561 gacagtgttt tcttttatga agatggttcc tttaatttgt tatacgactt tggcgcagaa
    13621 accggtgata caataacgct ttcttacttt ccgacctatg atggttcacc gttgaaaatg
    13681 attatagatt cagttggaat tataataatt aacggtaatg atagaaaaat tcaatatatc
    13741 acatgcggtg acgggatttt agtagaattt ggcaatgaga taattgaaga tatcgggagt
    13801 acacacaata tgtttcctgt atacgatggc actttaaatg gccctcttcg ttgctatgaa
    13861 gacagtataa ctggtcagta tttaagcggc tttcaccctg actatggttg gaatttcaaa
    13921 gattgtgccg aaataataac caggattaat gaagtgaaag actatgatgg aatttttctt
    13981 tatccaaacc ctgcatcaag ctctattacg gtaaaaaatg ttgatttacc aaaaaacttt
    14041 aaaataatta acatttttgg tcaggttatg aatgagggtc aactaatgac ttcgcgtacg
    14101 atatatctga ccggagtagc caatggagtt tattatctta ttttgcatga aggctcttat
    14161 caggaagaat atcaacaaga taatcgatat aaatttatca tttgtcatta ataaacacca
    14221 tagaaatgta catacaaata aagttaagaa atttagtagt cgtattaatt atgattacaa
    14281 gtcatatttg ttcttcacaa gatcctttta actatggaca aatatgtaac tctgaaccag
    14341 aatcatctcc gattgaaaat gctccttttt atgtttgcag tagaacgact ccgttctggt
    14401 caacaagcaa ccgtttgcat tttacgccat cggatagtaa tatttatata gctcacctaa
    14461 acattataat agtacaaaga aatggcgagg atccaggtaa ttggtctgat aacgaattgt
    14521 ttcgagatat tatatctaga gcaattgata cattgaatta ttattatagt aatctatgta
    14581 tcgtcccgat tattgaatat cccaattgtt ttgatacgac acttggagag ccagattata
    14641 ttccatcaat aaatattcag tttacttatt caatctattt catagccaac gaaaattatt
    14701 ggggatcagt caatccagat acctcgtgga gtagttattt aaattctcat gatccttgga
    14761 gaagaggcat taacatatat tatacaatgg attcaacaac atatcaatta ttggttgaag
    14821 aaaatcaatt ggacccaaat atgatctctt acaattgggt taattctatg cctagtgaaa
    14881 ctaatatgga gcgtaaacaa tatatggtat tgccaaataa atacgcagag tttgtttata
    14941 ttttaaataa ctacatccct gatacggctc aagttattag taatacagta catggttatg
    15001 caagaatctg gaagcatgaa ttgggtcata tatttggttt aaatcattca tcaattccag
    15061 ata
//
LOCUS       DAOPNH010000042        15028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3972_length_15028_cov_6.769652, whole genome shotgun sequence.
ACCESSION   DAOPNH010000042 DAOPNH010000000
VERSION     DAOPNH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3972_length_15028_cov_6.769652"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKZ74_03650"
     CDS             complement(<1..937)
                     /locus_tag="PKZ74_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPM91881.1"
                     /translation="MNKLLPVLLFVFCSVRIFSQEYPYQDPELPMDQRIADLLSRLTT
                     EEKISQLLYNSPAVGRLGIPEYNWWNECLHGVGRAGRATVFPQAVGLAATFDTSLVRR
                     IGDAISTEARAKYNLAVRKGNREQYMGLSFWTPNINIFRDPRWGRGQETYGEDPFLTG
                     RIGAAFVRGLQGSDPKYLKAAACAKHFAVHSGPEKTRHEFNALPDTTDFRETYLPAFK
                     TLVDAGVEAVMCAYNRLYDEPCCGSPFLLNDILRTDWGFKGHIVTDCWALDDIWLRHK
                     VVPTREEAAAMAARAGVNLNCGYIYQYLPQAIEKGL"
     gene            1123..1821
                     /locus_tag="PKZ74_03655"
     CDS             1123..1821
                     /locus_tag="PKZ74_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPM91882.1"
                     /translation="MMMRAHIFQHLAFEGPGTIQPWLKNAGYDLTFTVFAEKVYFPEI
                     NSVDFLVIMGGSMSVNDEDKFPWLIAEKQFIREFIASGKPVLGICLGAQLIASALGAR
                     VYPNKTKEIGWFPVTAIKPPQISNFQFPASFTTLHWHGETFDLPERAALLASSEATKN
                     QAFQYGKNVLAIQFHPEANMQTLQDFCFHFKKELVPGRFIQSEEEIVKYADEYLSSMD
                     DLIDRLMLYLVGDE"
     gene            complement(1916..3337)
                     /locus_tag="PKZ74_03660"
     CDS             complement(1916..3337)
                     /locus_tag="PKZ74_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPM91883.1"
                     /translation="MKKSLLIILCSLFTLHGSLAQNERMQWFKDAKLGIFIHWGIYAV
                     NGIDESWSFYNELLPYEDYLKQLDGFGAENYDPQAWAGLIRESGARYAVLTSKHHDGV
                     ALWDTDAGDLSVVKRTPAGKDLIGPFCSALRAEGLKVGLYYSLLDWSHPDYPNFTRNR
                     KRYERDSIPSRWDNFDRFNRAQITEITTRYNPDLVWFDGDWEQSAEVWKAAEIREMLL
                     NNNTNVIINSRLQGYGDYATPEQGIPVSRPKDEYWELCMTMNDSWGFQPNDTNYKPVN
                     LIIRIFADVIGMGGNLLLDIGPKADGTIPEEQVAILKELGRWTSKHSEAIYGTLAGLP
                     KDCYYGSSTLSADSTVLYLFIPSNSKSQVSNSKSQVPNSKQNPNLKSENPIENPQILK
                     SPNPQISQSSYSPAILLKGITNKINSIRVVGNGTMLEQTTWLKPWWSSNPGLIGISIP
                     AEAMDPDMTVIAVEFEGKLKVKF"
     gene            complement(3729..5207)
                     /locus_tag="PKZ74_03665"
     CDS             complement(3729..5207)
                     /locus_tag="PKZ74_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91884.1"
                     /translation="MIRLVFLSILLFFIGPLYTLCQSTFTYTISTPYDERVFNCAEAE
                     NGNLFFVGIRYYPDTISDAAYFLALDQDGNLLFEQEFFTEDTSLYLNDISVIGDSICL
                     FGAKGLTTLGKKNELWMLLLDQNYNIIMNKSMRLEDASIGGVEAIINTKGNFVFCCTI
                     APSELERNIFMSEVSSNFDSINSSILYLDGREFGFDLLETTDNIYKVFAYGNFPGAPN
                     TLGSIVLFDSMFNYISADSLPYGLHFQHTAQWLNDSVYLVTGEKFLDGGGKRDIGICK
                     MTEDDQFIAGNHFGKGGDTITKAGVSSNMDFNDSGNIYFGGITNVNIAYGIYQHDSWL
                     ILENIDSNLNQNWQRFYGGDAGYYLWALKATADGGCVMMATRYDPEVQDGETDVTIIK
                     VDSNGLLTSTGNGPAIPAQQLAIHPNPASRKITIAYPDIFKFQQKEILIYNIYGNKIY
                     QHDITADISRSYADISSYPNGLYIVVLSVEGKKIASGKVIKY"
     gene            complement(5328..6206)
                     /locus_tag="PKZ74_03670"
     CDS             complement(5328..6206)
                     /locus_tag="PKZ74_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91885.1"
                     /translation="MTNPTSATEQKIRDFLEGTNQMKGEQTYTIEEAIWYCEASLNFT
                     YAIYDSTFNFLSHETCRLSIDLNENSTVNENELMDAYEKLVDSLESHYDGILTTPKHV
                     MICDVKYDGVTSGQLDISMISVIAFGTSPSPYGSFGPTDYWWSGDEDGKCNGYSGIGD
                     ATTALENKMNNTLVAPAENVRIYYTDIESVEYVNPEDYPYEYGPRGLRGYAFADDDPE
                     AGVQCLPPGELNFYISNNGIPYIIDDNDTFEDLEFCEIDVIWDYLNDPNDYYEEHFYT
                     LMYGERHETNISATDL"
     gene            6704..7921
                     /locus_tag="PKZ74_03675"
     CDS             6704..7921
                     /locus_tag="PKZ74_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPM91886.1"
                     /translation="MEKAFTPITLGNLTIRNRFIKTATNEGMWENGLPAQSLIDHHAR
                     LAKGGVGLTTVAYGAVNADGRTMEHQMYMRPEVVPGLKRLTDAVHAEGGAASIQLTHC
                     GFFTNNRLVSNKRPVAPSRIINNYGLLSGIFCSREMTTADCRLLTADFARSARLALDA
                     GFDAIELHMGHGYLLNQFLSPRTNKRKDRYGGSLENRMRFPLEVVEAILRDESPRDGV
                     PRDEVPRDGVPLICKINLDDGMKNGFTIHDSIELAKQLEQAGVSLLVLSGGFTSHTPF
                     YLMRGDVPLKEMIRAEKQWQHKGAFAVFGRFIIRKYEFSENFFLPLARQIRKAVKMPL
                     AYLGGVVSSKGIEQVFSEGFDMVAIGRALIHDPDFILNAAKDPGHTSGCTHCNICVAE
                     MDKNGVRCVLNVR"
     gene            complement(7933..9057)
                     /locus_tag="PKZ74_03680"
     CDS             complement(7933..9057)
                     /locus_tag="PKZ74_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM91887.1"
                     /translation="MESPFVFGRKATGNNFTDREKETGRLISNFNNRVNTILISPRRW
                     GKSSLVRRAGELAGNESLKVIYFDSFSLRDPNEFYKVLVTETIKGTSNSIETWIDHIK
                     RFFNRVTPKVSFGPDPLSTFELSFELEAVEKNYQELLDLPEKIATDKGLKLVICIDEF
                     QNTAIFNESSLFHKRLRAAWQNHQNVTYCIYGSKQHMMTTLFEKQSMPFYKFGEIIHL
                     EKIAVSDWVSYITDRFRDTGKMIPVPLAEKIALTVKCHSYYVQQLSHLIWLRTEKIPD
                     EVIFFSAVDDLLSQNAMLYQRETELLSDTQINFLKALASGVSTGFNSAEILNKFRLGS
                     SANASKVKKTLVDRELINIHNQNAEFLDPAYELWFIREIR"
     gene            9188..10438
                     /locus_tag="PKZ74_03685"
     CDS             9188..10438
                     /locus_tag="PKZ74_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM91888.1"
                     /translation="MLFPLYIAKRYLIAKKSHNIINIISIISVIGVMVGTGALIIVLS
                     VFNGFEGLVMGLFNSVNPDLKVTADEGKVFTLTEEQLSRIKSIPGVAYVVETVEETAL
                     ARFGDSQCIVKLKGVSDEFTEMTPLDSFMLNGKYTLGSPQRPASVIGAGVAYYLGIYP
                     EEFAVPVMLYLPKRTAKTLSGLPDQSFNSRPVRVAGVFSVQQEFDMSYVIAPIELARE
                     LLEYDREVTALELGISDPGATGSIEKQVAEITGPGFLIKDRFQQQATLYKVMKSEKWA
                     VFFILALILVIAAFNMIGSLSMLIVDKKKDIAVLWSLGATKSLIRRIFFTEGMMISLA
                     GGLLGLCLGGLLALLQQEFGLLKLGGGEGNYIVDAYPVKVQLLDFFYVLLTVILIGAA
                     TTWYPVRQISRKYLAQRMGVFFSR"
     gene            10697..14179
                     /locus_tag="PKZ74_03690"
     CDS             10697..14179
                     /locus_tag="PKZ74_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM91889.1"
                     /translation="MKIFIQISRIITVFLFSLTISASANSIKLNNNENDIRIIQKTGD
                     GFRMEVTLSEFKTMDVKTDKGIFTRIQVPAWSRSRAYGQPEMPVKSELIEIPAGAVVK
                     INLVSWEVNEYRLRDLDISYALMPSQPPVAKSGKIPAFMYERDVYKIHAFIQSEIVSV
                     EELGTMRGVRIGRLDISPFEYNPGEGIVRIYEKLEFDVIFENADPAASERNKLTYGNQ
                     YFAPVFGSLINYTSPKVSERENLTQYPVKYVIVSDPMFEDQLQPFIEWKTKKGFQVIE
                     AYTSDPNVGGTTSQIKAFLQNLYESATPEDPAPSFVLFVGDVAQIPVFYGQAASHETD
                     LYYCEYTGDYFPEMFYGRFSAQNAAQLQPQIDKTLMVEQYTMPVTSYQDTVVMIAGMD
                     GTFGPTHGNGQINYGTINYFNAAHGLSSHTYLYPESGGNADNIRQNISDGVSFANYTA
                     HGSPDGWADPSFTVGDIPSLQNNGKFGLLIGNCCSTSEYEVSNSFAEAIVRAADKGAV
                     GYIGASNSTYWDEDYYFGVGVGTISGDPPSYEETTLGDYDRMFHTHGEPFSEWYTTMD
                     QVIYAGNLAVTLGSPGMAEYYWEAYCLMGDPSLTVYLGEPPVLTASFDPLLPLGNMSF
                     IVNTVPYAYVGLSMNGELLGASLADSNGVATVILTTLPQPGNADVVVTAQNYQPFTGT
                     VLIANPEGPYVSLNQHVPNDENDNGMIEFGEEITLDIELKNWGNDDATGTMASLSTND
                     QYVTISDETENYGTIPAQDSVMQMAAFSFEVAGYVPDMHVVPFDVTIQDQTREVWTSS
                     FMLTLFAPVMEIGAMLIDDTEGGNGNYRLDQGETIIIIVDCHNTGHCDAMNSHASLYS
                     NSPFITLGTASYVFDTLSSGGMSQAIFTATLASEIETGTLIDLTVMLDSDPYGDMTVF
                     LPKVGLVMEDFETGNFDSFGWEMGGTQPWITTTENVFDGTYSAVSGDISDLQTSELML
                     EMETAVDDSISFFRKVSCEDDPYNDNYDWLAFYIDGNEMDRWDGEVNWGRVAYPVTAG
                     LHTFKWVFLKDYSVASGFDAAWIDNVIFPAPAPVINVREIQPSGHLQFTLNPNPAGEM
                     TNIFLTLDSQMEITVNLYDQKGNFIRRLLDNVRIMDGSATITISTGDLANGIYYCTVE
                     AGGQSFTRKLVINN"
     gene            14208..>15028
                     /locus_tag="PKZ74_03695"
     CDS             14208..>15028
                     /locus_tag="PKZ74_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HPM91890.1"
                     /translation="MKRTLLSIFLSFITIWLFAGNGYQVEYSQPKAGVHLLEFTLDGY
                     GVSQVELQGQTYSRIEFEGKVVTQKKGFAELPYLNASVMIDPVKNVFIGIIPGEYEEF
                     LLEYPLVPSRGVIYRDQDPASVPYAIDPKSVTDTWYPVVLAEHTDPFILRDIRGTSVY
                     AYPFQYNPVKQVLRVYKTMKIRVVENNSAVINPLGKAPEKIVREMDAVYRSVFINYNH
                     NRDNLTIGEYGDIHVIVTSRDEDAIQPYVDWKKEKGYDVSVEVVPAGTVVNNNVQA"
BASE COUNT         3880 a         3601 c         3541 g         4006 t
ORIGIN      
        1 tcagcccttt ctcgatagcc tgcggcagat actggtaaat gtacccgcaa ttcaggttga
       61 cgccggcccg ggcggccatg gctgccgcct cttctcgcgt cggcaccacc ttgtgcctga
      121 gccagatatc gtcgagggcc cagcaatcgg ttacgatatg gcctttaaat ccccaatctg
      181 ttcttaaaat atcattcaat aggaacgggc tgccgcagca aggctcgtcg taaagcctgt
      241 tgtatgcaca cataactgct tcaaccccgg catcgacgag cgttttaaag gccggcaggt
      301 aagtttcgcg aaagtcggtc gtgtcgggca gagcgttgaa ttcgtggcgg gttttttccg
      361 gcccgctgtg tacggcgaaa tgtttggcac aggcggctgc cttgaggtac ttcgggtcgc
      421 tgccctgcaa tcccctcaca aacgccgcac ctatccggcc tgtcaggaac gggtcttcgc
      481 cataggtctc ctgtccgcgg ccccagcgcg gatcgcggaa aatattgatg ttgggcgtcc
      541 agaacgagag gcccatgtat tgttcccggt tgcctttccg caccgccagg ttgtattttg
      601 cacgggcttc ggttgagatg gcatctccga tccgccggac aaggctcgta tcaaaggttg
      661 ccgccagtcc gacggcctgc ggaaagacag ttgcccgccc cgcccttccg acgccgtgaa
      721 ggcactcgtt ccaccagtta tattcgggga tgcccagccg cccgaccgcc gggctgttgt
      781 acaggagctg cgatattttt tcttcggttg tcagtcgcga aagaaggtca gcaattcgct
      841 gatccatagg taattcggga tcctgatatg gatattcctg tgaaaagatt cgtactgagc
      901 agaaaacaaa cagtaaaacc ggcagtaact tattcatctt taatattatg tgtttactct
      961 cccgccttgc gggacaactt gtgtaaatcc gtgtaatctg tggtgaaaac tttaactcac
     1021 cacggagtgg cacaaagtta cacaacttgt cccgcatgga gggagagtta gcatagagtt
     1081 tacaccagga ttatcattgt taatattagt tttgtcaaaa aaatgatgat gcgtgcacac
     1141 atctttcagc atctcgcctt cgaaggcccc ggaaccatcc agccctggct gaagaatgcc
     1201 ggctatgatc tcacttttac cgtttttgct gagaaagtat atttccctga gataaattcg
     1261 gtcgatttcc ttgtcatcat gggcggatcg atgagtgtga acgatgaaga caaatttccc
     1321 tggctcatcg ctgaaaagca atttatccgg gaatttatcg cttcaggtaa gccggtcctg
     1381 ggcatctgcc tcggtgcgca gctcattgcc agcgccctcg gcgcccgggt ctatcccaac
     1441 aaaaccaaag agatcggctg gttccctgtg acggcaataa agccacccca aatttccaat
     1501 ttccaatttc ctgcttcatt cacgaccttg cactggcacg gcgaaacgtt cgacctgccc
     1561 gaaagggcag cgctcctggc cagcagcgaa gccacaaaaa accaggcctt tcagtacgga
     1621 aaaaatgtcc tggccatcca gttccacccc gaagctaaca tgcagacgct acaggatttc
     1681 tgttttcatt tcaagaagga gctggtgccg ggaaggttca ttcagagtga ggaagaaatt
     1741 gtcaaatatg cggatgaata cctttcttca atggatgatc ttattgatag actgatgctt
     1801 tatctggtcg gagatgaatg attattttca ccacataggc acatagaaca catagaaaac
     1861 aaattagatc ctatgtgatc ctatgtgctc tatgtgccta tgtggtaaat aattatcaga
     1921 acttcacctt aagtttccct tcgaattcta ccgcgatcac tgtcatatcc gggtccatcg
     1981 cctctgcggg aatggaaatc ccgatcagcc cgggattgct gctccaccac ggcttcagcc
     2041 aggtagtctg ctccagcatt gtgccgttgc cgacgacccg gatgctgttg atcttgtttg
     2101 taattccctt cagcaggatg gcagggctgt aagacgattg ggagatttgg ggatttggtg
     2161 atttgaggat ttggggattt tcgattggat tttcggattt gagatttgga ttttgtttgg
     2221 aatttggtac ttgggatttg gaatttgata cctgggattt ggaatttgac ggaataaaga
     2281 gatacaaaac agtgctgtca gcagataatg tcgaactgcc atagtagcaa tccttgggca
     2341 gccccgccag cgtgccgtag atcgcttcgc tgtgtttcga agtccaacgg cccagttctt
     2401 tcaggatagc gacctgctct tccggtatcg tgccgtcggc tttcgggccg atgtcgagca
     2461 gcaggttgcc gcccatgccg atcacgtcgg caaagatgcg gatgatgagg tttaccggct
     2521 tgtagttcgt atcgttgggt tggaaacccc aggagtcgtt catggtcatg cacagttccc
     2581 agtattcgtc tttcggcctt gaaaccggga tcccctgctc cggcgtggca taatctccat
     2641 atccctgcaa cctgctattg ataatgacat tggtattgtt attgagcagc atctccctga
     2701 tctccgccgc tttccacacc tccgcgcttt gttcccagtc gccgtcgaac cacaccaggt
     2761 cgggattgta gcgcgttgtg atctcggtga tctgggcccg gttgaaccgg tcgaaattat
     2821 cccagcgcga ggggatggag tccctctcgt agcgtttccg gttgcgggta aagttcgggt
     2881 aatcgggatg cgaccagtcg agcagcgaat agtacagccc gactttcagc ccttccgccc
     2941 tcagtgcgct gcagaacgga ccgatgagat cttttcccgc cggcgttctc tttaccaccg
     3001 acaggtcgcc ggcatcggtg tcccacagcg caacgccgtc gtggtgtttc gaagtcagaa
     3061 cggcatagcg ggcgccgctt tcccggatca ggccggccca ggcctgcgga tcgtaatttt
     3121 cggcgccaaa gccgtcaagt tgcttcaagt aatcttcata gggaagtaat tcgttgtaaa
     3181 acgaccacga ctcgtcgatg ccattcacgg catagatccc ccagtggatg aaaattccga
     3241 gtttggcatc tttgaaccat tgcatccgct cattctgagc aagtgaaccg tgaagggtga
     3301 agagtgaaca gagtattatt agtaaagatt ttttcataat ggtttatatt tcaatcaaat
     3361 ttatattgaa ttccggatga ttcagaaata tcacctgaga attcgtatct ttagcaggca
     3421 gataccaggc taacattttt acaatgaaat cattcttaat ttgttgcgca ttaatcgtca
     3481 tttcaggttt tgccctttgc caggtttatc ctcctccggg ctgcatttcc tatgaaaacg
     3541 gtgtgcttac catttgtccg cccgattcgg tgccggagtg tatctgctcc gatttgaggt
     3601 ggataagttg aatttttacc ggaaggtttg tgtcgctaat ggttattaag cggttgcttc
     3661 ttattttgat aacaacttag acactgagtt cactaagagg cacttagagg aaatgctgcc
     3721 ggccttcatc aatattttat cactttcccg gatgcaatct tctttccttc aacgctgagt
     3781 acaacgatat acaatccgtt gggataacta ctgatatcgg catatgatct tgatatatcg
     3841 gcagtgatgt catgctgata gatcttgttt ccgtaaatgt tataaatcag gatttctttt
     3901 tgttgaaact taaagatatc agggtatgcg attgtgattt ttctggatgc gggattcggg
     3961 tggatggcaa gctgttgggc ggggatagcc ggtccattcc cggtagatgt aagcaatcca
     4021 ttggaatcga ctttgataat ggtgacgtcg gtttcgccat cttgtacttc gggatcgtaa
     4081 cgggtggcca tcattacgca tccgccgtcg gccgtggcct taagtgccca aagataatag
     4141 ccggcatccc caccgtagaa tctttgccag ttctgattga ggtttgaatc gatattttca
     4201 aggatgagcc acgaatcatg ctgataaatg ccataggcga tattaacatt ggttatgccc
     4261 ccaaagtata tgttccctga gtcattaaaa tccatattag aagatacccc tgcttttgta
     4321 atagtatcac cccctttccc aaaatgattg ccggctataa attgatcatc ttccgtcatt
     4381 ttgcaaatgc caatatctct ttttccacct ccgtctaaaa atttctctcc ggtaaccaga
     4441 tacaccgaat catttaacca ttgcgccgta tgctggaaat gaagcccgta aggcagggaa
     4501 tccgccgaga tgtagttgaa cattgaatcg aataaaacaa tggatccaag ggtatttgga
     4561 gctcccggaa aatttccata agcaaacact ttgtaaatat tatcggttgt ttctaataaa
     4621 tcaaatccaa actcgcggcc atccaggtaa agtatcgaac tatttatact gtcaaagttt
     4681 gatgatacct ccgacataaa aatgtttctt tcaagttcgc tgggcgcaat tgtacagcaa
     4741 aaaacgaaat ttcctttcgt gttaataata gcttcaacac ccccgataga ggcatcttcc
     4801 aggcgcatag atttattcat tattatatta taattctggt caagtaaaag catccataac
     4861 tcgtttttct tacccaaggt cgttaatcct tttgctccaa acaaacagat cgagtcgcca
     4921 atcacactaa tatcatttag atataaagaa gtatcttcag taaaaaactc ctgctcaaac
     4981 aataaatttc catcttgatc tagcgccaga aagtaagcag cgtcagaaat ggtatctgga
     5041 tagtatctaa ttcctacaaa aaataaatta ccattttccg cttctgcgca attaaatact
     5101 ctttcatcat aaggtgtact gattgtgtat gtaaaggttg attgacacaa agtatacaat
     5161 gggccaataa agaatagaag tattgataaa aacactaatc ttatcatatc cagggattta
     5221 tataaatatt cattatgaat cctgcaataa agatcaagga atcaggtaaa aagtaacact
     5281 gccagttgat acttgagttt attgcaggat gattctaaaa ttatcaatta cagatcggta
     5341 gctgaaatat tcgtttcatg ccgctcaccg tacattaaag tataaaaatg ttcttcataa
     5401 taatcatttg ggtcatttag gtaatcccaa attacatcta tttcacaaaa ttccagatcc
     5461 tcgaaggtgt cattgtcgtc aataatgtaa gggattccgt tgtttgagat atagaaattc
     5521 agttcgccgg gaggaaggca ttgaactcct gcctcgggat catcgtctgc gaaagcgtag
     5581 ccgcgaagtc ctctcggtcc gtattcatat ggataatctt ccggattgac atattcgaca
     5641 gactcaatgt cagtataata tatcctgaca ttttctgccg gagcaaccag tgtattgttc
     5701 attttatttt ccagcgcagt ggtggcatct ccgattccag aataaccgtt gcatttaccg
     5761 tcctcatccc ccgaccacca ataatcggtt ggcccgaacg atccgtaagg acttggagag
     5821 gtcccgaatg caataaccga gatcatcgaa atatcgagtt gtcctgaggt cacgccatcg
     5881 tatttcacat cgcagatcat gacatgcttg ggggttgtca atatgccatc gtaatgggat
     5941 tcaaggctgt caaccagttt ttcgtaagca tccatcagtt cattttcatt caccgtgctg
     6001 ttttcgttca ggtcaatact caaccggcag gtctcgtgcg aaagaaaatt aaatgtggaa
     6061 tcgtatatgg cataggtaaa gttcaatgaa gcttcacagt accagatcgc ttcttcaatg
     6121 gtataggttt gttcgccttt catttgattt gtcccttcaa ggaagtcccg gattttttgt
     6181 tctgttgcac ttgtcgggtt ggtcataaca ggctgttttt ccggttcaag gatttttttg
     6241 caactaaaaa taacaaatac tgacaaggca aatagagata gtaataaata agtgcttttc
     6301 attttgttaa aattttaata ttaatattgg attaactgaa gtcaggcaaa aatgaaaaaa
     6361 cctaacctaa ggaggaaaaa gatgtttaat gatgagaatg aggttaatac tctctctctc
     6421 tctctctctc tacagcaatg catattagat gaattatgga attcaaaggg catcaggaat
     6481 taatggttaa aacaaaatgg ctgttaaaga taattaaata aaatagatat gtcaagcaaa
     6541 tcttcgaata attttcaaca gaaatgaaat tttatcagaa gtgcatcctt tcattttcct
     6601 gccgagatga aaaatgtaaa atgaaaaata ccaaagaaat gtcagtggaa atttccagat
     6661 catataagaa ggaatttcta ccttcgcccg aaattctttc accatggaga aagcatttac
     6721 acccatcacc ctgggcaacc tcaccatccg caaccggttc atcaaaacgg cgaccaacga
     6781 agggatgtgg gaaaacggtt tgccggcgca gtcgctcatc gatcaccatg ccaggcttgc
     6841 gaaagggggc gtcggtctta ccacggtggc ttacggcgct gtcaatgccg acgggcggac
     6901 gatggaacac cagatgtaca tgcggccgga ggtggttccg ggattgaaac ggctgaccga
     6961 tgcggtccat gctgaaggtg gcgcggccag tatccagcta acacattgcg gtttcttcac
     7021 caacaaccgg ctggtcagca ataagcgccc tgtcgccccc agccgtatca tcaacaacta
     7081 cggcctcctg agcggaatat tctgctccag ggaaatgaca acggccgact gccgactgtt
     7141 gactgctgac tttgcccgct cagcgcgcct tgcgttagat gcgggcttcg acgccatcga
     7201 gctgcacatg ggacacggtt atcttctcaa ccagttcctc agcccccgca ccaacaagcg
     7261 caaagaccgg tacggcggga gcctggagaa ccggatgcgg tttccgctgg aggtggtgga
     7321 ggctatccta agggacgaaa gcccaaggga tggcgtccca agggacgagg tcccgaggga
     7381 tggcgtccct ttgatctgca aaattaacct cgacgacgga atgaaaaatg gatttaccat
     7441 ccacgactcg atcgagctgg ccaaacaact cgaacaggcc ggcgtcagcc tcctggtgct
     7501 cagcggcgga tttaccagcc acactccgtt ctacctcatg cgcggcgatg ttccgctgaa
     7561 agagatgatc agggcagaga aacaatggca gcataaagga gcctttgccg ttttcggacg
     7621 ttttatcatc aggaaatatg agttctccga gaacttcttc ctccccctcg cccggcagat
     7681 acgcaaagcc gttaagatgc cgctggccta cctcggtggc gtcgtctcat caaaaggcat
     7741 tgaacaggtt ttctccgaag gtttcgatat ggtggccatc ggacgcgccc tgatccacga
     7801 cccggacttt atcctgaacg cagcaaagga tcccggtcac acctccggat gcacccactg
     7861 caacatctgc gtggcggaaa tggataagaa cggggtaagg tgtgtgctga atgttagata
     7921 atttgtttga tcttatcgaa tttcacgaat aaaccataat tcatatgccg gatccagaaa
     7981 ctcggcgttc tggttgtgaa tgttaattaa ttccctgtcg accagtgttt tcttaacttt
     8041 acttgcattt gcggaactcc ccaaccggaa tttatttaat atttctgctg agttaaaacc
     8101 ggtacttaca ccggatgcca gcgcttttag aaagtttatc tgcgtatcgc ttaaaagttc
     8161 ggtttcacgc tggtagagca tcgcattctg cgagaggaga tcatcaaccg ctgaaaaaaa
     8221 gatcacttcg tctggtattt tttctgttcg cagccagata agatgtgaaa gctgctggac
     8281 atagtatgaa tgacatttta ccgtcagtgc aatcttctct gccagaggaa caggaatcat
     8341 ttttcctgta tcacggaaac ggtctgttat gtaagaaacc caatctgata cggctatttt
     8401 ctcaagatgt atgatctccc caaatttata aaacggcatg gattgttttt caaataaagt
     8461 tgtcatcatg tgttgcttac ttccataaat acagtacgtt acgttttgat ggttttgcca
     8521 tgcagcccga agacgcttat gaaaaaggga cgattcgttg aagatagccg tattttgaaa
     8581 ttcatcgatg caaataacca gcttcaggcc tttatctgtt gcaatttttt caggcagatc
     8641 aagtaattcc tggtaatttt tttcaactgc ttccagttca aatgaaagtt caaaagtgct
     8701 taacggatca gggccaaaag atacctttgg ggttacccgg ttaaaaaatc tcttaatatg
     8761 atctatccat gtttcaatag aattagatgt tcctttgata gtttctgtga ccagaacctt
     8821 ataaaattca ttgggatcgc gcaaactgaa agaatcaaaa taaatgactt tcaatgattc
     8881 attcccggcc agttcgccgg ccctcctcac caacgaagat tttccccatc gtctgggtga
     8941 gatcaatatc gtgtttacac ggttattaaa atttgagatt aatctcccgg tttctttttc
     9001 cctgtcagtg aagttattac cagtggcttt tcttccaaat acgaacggac tttccatata
     9061 aatttgattg aaatggtgga gaagcaaaga tacaacattt atatgtaacc tatcggttgc
     9121 aaactaatgg gtttgtaact gaaagctgcg gcgctgtttc gtcttataaa atgcctaaat
     9181 ttgcaccatg ctctttcccc tctacatagc aaaacgatac cttatcgcga aaaaatcgca
     9241 caacatcatc aacatcatct cgatcatttc ggtcatcggg gtgatggtgg gaacgggcgc
     9301 actgatcatc gttttgtcgg tattcaacgg gttcgaagga ctggtcatgg ggctgttcaa
     9361 cagcgttaat cccgacctga aagtgacagc ggatgaaggt aaagtgttca cgctgaccga
     9421 agaacagttg agccggatca aaagcattcc gggcgttgcc tatgtggttg aaaccgtcga
     9481 agaaacagcc ctcgcccgtt tcggcgacag ccagtgcatt gtgaagctca aaggggtcag
     9541 cgatgaattt acggagatga cgccgctcga cagcttcatg ctcaacggaa agtacaccct
     9601 gggcagcccg cagcgccccg catcggtgat cggggccggc gtggcctact acctgggcat
     9661 ttacccggag gaatttgccg taccggtgat gctctacttg ccgaaacgga ccgccaaaac
     9721 gctctcaggc ctgcccgacc agtcgttcaa cagccggccg gtgcgcgtag ccggcgtctt
     9781 ctccgttcag caggaattcg acatgtcgta cgtgattgcc cctatcgaac tggcccgcga
     9841 actgctcgaa tacgaccggg aagtgaccgc cctcgagctg ggcatctccg acccgggagc
     9901 aaccggcagc atcgaaaagc aagtggcgga aattaccgga cccggctttt tgatcaaaga
     9961 ccggttccag caacaggcga ctctctataa agtcatgaag tcggaaaagt gggctgtctt
    10021 cttcatcctg gccctgatcc ttgtcatcgc ggcttttaac atgatcggct cgctgtcgat
    10081 gctgatcgtc gataagaaaa aagatatcgc ggtgctctgg agcctcgggg ccaccaaatc
    10141 gctgatccgt agaatcttct tcaccgaagg gatgatgata tccctggcag gaggactcct
    10201 cggcctctgc ctgggcggat tgctggcctt gctccagcag gaattcggcc tgctgaaact
    10261 gggcggcggt gaaggcaatt atatcgtcga cgcctaccct gtgaaagtcc aactgctcga
    10321 ttttttctac gttctcctta ccgtcatcct gatcggcgcc gccaccacct ggtacccggt
    10381 gcggcagatc tcacgcaagt atctggccca gcggatgggg gtgttcttta gtcgctaggg
    10441 aatgatagcc ggcattttct tccgacgggt aaacccatcg gttccgaatg ttttataagg
    10501 gttggaaccg acggctttag tcgtcggact acctaaaaaa gtgaatatat agacttttga
    10561 gacatcctta atacgatatt catcatattt caatgaaatc ataagatcat tacctgaaaa
    10621 aatctacttt tgttttatta ataatgactt tttccctttc gacgcttaac cataatgagt
    10681 aattaatcaa atgtttatga aaatttttat ccaaatctca aggattatta ctgtattcct
    10741 tttttcgtta actatttcag ccagtgccaa ttccatcaag ctgaacaaca atgaaaacga
    10801 tatccggatt atccaaaaaa ccggcgatgg tttcagaatg gaggtgactt tatcagagtt
    10861 taaaacgatg gatgtcaaga ccgataaagg gatcttcacc cgtatccagg tcccggcatg
    10921 gtcgaggtcg cgtgcatacg gacagccgga aatgcccgta aaaagcgagc tgatcgaaat
    10981 accggcaggc gctgttgtga aaatcaatct ggtcagctgg gaagtgaacg aataccggtt
    11041 gagagacctg gacatttcct atgccctgat gcccagccag cccccggtcg ccaaatcggg
    11101 taagattccg gcgtttatgt atgaaaggga tgtttacaaa atccatgctt tcattcaatc
    11161 ggaaatcgtt tcggtggaag aacttggaac catgagaggg gtcaggatcg gtcgcctgga
    11221 tatcagcccc ttcgaataca atcccggaga agggatcgtc aggatttacg aaaaactgga
    11281 gtttgatgtc atttttgaaa atgctgatcc tgctgcttca gaaagaaaca agctgactta
    11341 cggaaaccaa tattttgccc ccgttttcgg atcactcatc aattatactt caccgaaagt
    11401 ttccgaaagg gagaacctga cgcagtaccc ggtaaagtat gtcatcgtca gcgacccgat
    11461 gtttgaagac cagctgcagc cttttataga atggaagacc aaaaaaggat tccaggtgat
    11521 cgaagcttat accagcgatc cgaatgtcgg aggcacgaca tcccagatca aagcttttct
    11581 gcagaacctt tatgaaagcg ccactcccga ggatccggcc ccttcctttg tcctgtttgt
    11641 tggtgatgta gcccagattc cggtgttcta cggacaggca gccagccatg agactgatct
    11701 ctattactgc gaatataccg gagattattt ccccgagatg ttctacggcc gtttttcagc
    11761 ccagaacgct gcccagcttc agccccagat cgataaaaca cttatggttg agcaatatac
    11821 gatgcctgtg acatcctacc aggataccgt tgtgatgatc gccgggatgg atggcacttt
    11881 cgggccaacc cacggcaacg gccagatcaa ttacggcacg atcaattatt tcaatgcagc
    11941 gcacggcctt tcttcgcata cttacctcta tcccgaatcg ggcggcaatg cggacaacat
    12001 ccggcagaat atcagcgacg gcgtgagctt tgccaactat acagcccacg gttctcccga
    12061 tggctgggcc gatccgagct ttaccgtcgg cgatatcccc tcgctgcaga ataacggaaa
    12121 atttggcctg ctgatcggta attgctgctc caccagcgag tatgaagtga gcaacagttt
    12181 tgccgaagcc atcgtcaggg ctgccgacaa aggcgctgtc ggatacatag gcgcatccaa
    12241 cagtacttac tgggatgaag actattattt cggtgtcggt gtcggaacga tttcgggcga
    12301 tccgccttcc tatgaagaga cgaccctggg cgattacgac cgcatgttcc atacccatgg
    12361 agagcccttc agcgaatggt acaccaccat ggaccaggtg atctatgccg gaaacctcgc
    12421 agttaccctg ggttcgccgg gtatggccga atattactgg gaagcctatt gcctcatggg
    12481 cgatccgtcg cttaccgttt acctcggcga accaccggtt ctgacggcct ctttcgatcc
    12541 gctccttccc ctcggaaata tgagttttat cgtgaataca gtgccatatg cctatgtcgg
    12601 cttatcgatg aatggcgaat tgctgggagc ctctttagcc gattccaacg gcgtggcaac
    12661 ggttatcctt acaactttgc ctcaaccggg caatgccgat gtggtggtga ccgcacagaa
    12721 ttaccagcct tttactggga cagttttgat tgccaatccg gaaggcccgt atgtttctct
    12781 taaccagcat gttccaaatg atgagaatga taatggaatg atagagttcg gagaagagat
    12841 tacccttgac atcgagctga agaactgggg aaatgatgat gctaccggta caatggccag
    12901 tctttcaacc aacgaccaat atgtcaccat ttcggatgaa acggaaaatt acggaactat
    12961 cccggcacag gattcggtca tgcaaatggc tgcatttagt tttgaagtgg ccggatacgt
    13021 acccgacatg catgtggtgc ctttcgacgt gacgatacag gaccagacgc gcgaggtgtg
    13081 gacttcgtcg tttatgctga cattgtttgc tccggttatg gagatcggtg cgatgctcat
    13141 cgacgatacc gaaggcggta acggcaatta ccggctcgat cagggagaaa ccatcatcat
    13201 cattgtcgac tgccacaata ccggccattg cgatgctatg aacagccatg cttcccttta
    13261 ctcaaacagc ccttttatca cactcggcac agcttcttat gttttcgata ctttaagcag
    13321 cggcgggatg agccaggcca tctttacggc tactctggcc agtgaaattg agaccggaac
    13381 cctgatcgac ctgacagtca tgctggattc agatccttat ggcgatatga ctgtttttct
    13441 cccgaaagtg ggactcgtta tggaagactt tgaaaccggt aactttgatt ctttcggatg
    13501 ggaaatgggc gggacacaac cctggattac cacaaccgaa aacgtctttg acgggaccta
    13561 ttcggcggtt tcaggcgata tcagcgactt gcagacttcc gaacttatgc ttgagatgga
    13621 aaccgcggtc gatgattcga tctctttctt ccgtaaagtt tcctgtgaag atgatcctta
    13681 caatgacaac tacgactggc tggctttcta tatcgacggc aatgagatgg accgttggga
    13741 cggcgaagtt aactggggac gggttgccta tccggtcact gccggactgc atacattcaa
    13801 atgggtcttc cttaaggatt attcggtggc ttccggtttt gatgctgcct ggatcgataa
    13861 tgtcattttc ccggccccgg ccccggttat taacgtcagg gaaatccaac catccgggca
    13921 tttgcagttt acactgaatc caaaccctgc aggagaaatg acaaatattt tcttaactct
    13981 tgacagtcag atggaaatca ctgtaaattt gtatgaccag aaaggtaact tcatccgccg
    14041 gcttcttgat aatgtgcgaa taatggacgg atcggcaacg atcacaattt ccaccgggga
    14101 tctggcgaat ggaatttatt actgtaccgt tgaagcggga ggtcagtcat ttacccggaa
    14161 actcgtcatc aataattaat atattttgta aataactcat tactcatatg aaaaggacgc
    14221 ttctatccat ttttttatca ttcattacca tttggctttt tgccggtaac ggttaccagg
    14281 tcgaatactc ccagcccaaa gcaggggtcc atttgctgga attcaccctc gacggatacg
    14341 gggtgtcgca ggtggaactg cagggacaaa cctactcccg gatcgaattt gaagggaaag
    14401 tggttacgca gaagaaaggc tttgccgaat tgccctatct gaatgcttcg gtgatgatcg
    14461 atccggtgaa gaatgtgttt atcggtatca ttccgggaga atacgaggaa tttctgctgg
    14521 aatacccgct ggttccctcg cgtggcgtta tttaccgtga ccaggatccc gcctccgttc
    14581 cctatgcaat tgatccgaaa tcggtaaccg atacctggta ccccgttgtc cttgcagagc
    14641 ataccgaccc gttcatcctc cgagatatcc gcggcacgag cgtatatgcc tatcctttcc
    14701 agtataatcc ggtgaagcag gtgctcaggg tttacaaaac catgaaaatc agggtagtgg
    14761 aaaataattc ggcggtgatc aatcccctgg gcaaagcccc cgaaaagatt gtccgcgaaa
    14821 tggatgccgt ttaccggtcg gttttcatca attacaatca taacagggat aacctgacga
    14881 taggagagta cggcgacatc cacgtgattg tcacctcgcg cgacgaagat gctattcagc
    14941 cttatgtcga ctggaaaaaa gagaaaggct atgacgtttc cgttgaggta gtcccggcgg
    15001 gaacagtcgt caataacaat gtacaggc
//
LOCUS       DAOPNH010000043        14999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3986_length_14999_cov_7.844218, whole genome shotgun sequence.
ACCESSION   DAOPNH010000043 DAOPNH010000000
VERSION     DAOPNH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_14999_cov_7.844218"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="PKZ74_03700"
     CDS             <1..2349
                     /locus_tag="PKZ74_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM91891.1"
                     /translation="VRDTLKYGFIDEKINGKQGMRLLVMKQSGANTVSIARDVHAKMD
                     ELVKTLPPDISVNPIFDSSDFIKGSIDNLSETLMWAFLFVILVVLFFLGRWRATMIII
                     LTIPISLVVSFIYLYFTGSSLNVISLSSLSIAIGMVVDDAIVVLENITKHIERGSSPR
                     EAAIYATNEVWLAVIITTLVIVAVFLPLTMVGGMTGVMFNELGWIVTITIVTSTLVAI
                     TLTPMLSSKLLKTWIPKEKSMNSAYDKTIRKGLDKLDNFYERTIRWALHHKSLVIIVS
                     LAIFIGSLLLGAFFVKTDFMPESDESRMTVSIELQTGLRMEESKITAEKVEKIIRERY
                     PEITIISNSTGADDEGNFFSLFRPTGSNRINLMMRLVPVTERSRSVFEIADDFRNQLD
                     QIAEVVNYDIATSNNMMGGGSNTVDVEIFGYDFNETNRIADEIKTRISRIPGAKDVSL
                     SREKDRPELQIVLDREKLAMNGLNSALVSTYIRNRVDGMTASKFREEGDEYNIELRYQ
                     EDYRNSITDLEEITIINPSGQRIKLKELGEVKEYWSPPNIEHKRRERIAKVSVKPVGV
                     SLGELAESIRKEVASVEVPPEIMINVGGAYEDQMEGFQDIGLLMIMSLVLVFIVMASQ
                     FESFAMPFVIMFSIPFAFTGVVLSLFLTNTTLSLIAALGAVLLIGIVVKNGIVLVDFI
                     NLMRDRGFELYEAIARAGHSRLRPVLMTAGTTILGMLPLALSKGEGSEIWTPMGITVI
                     GGLVISTIVTMVIVPVMYAILARRGERDKKTRLRKRFLFMDK"
     gene            2361..2654
                     /locus_tag="PKZ74_03705"
     CDS             2361..2654
                     /locus_tag="PKZ74_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91892.1"
                     /translation="MKAVFIVFNQAHTERVEYLFDRLEIRGYTWWTDVHGRGSASGEP
                     RMGTHTWPEINSAAMAIVDDNAVDEILEKVRRIDEINREVGIRAFVWDILKSV"
     gene            2780..3067
                     /locus_tag="PKZ74_03710"
     CDS             2780..3067
                     /locus_tag="PKZ74_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91893.1"
                     /translation="MFHQVSDKRLAKAGRMETTLSPQGGGRVQSPPRTPEAERRRGAR
                     GSKDRTTPKGSAREGRPPKPGATEAKSAERRTRGREPDKEEKEPKGTGTKG"
     gene            3216..3551
                     /locus_tag="PKZ74_03715"
     CDS             3216..3551
                     /locus_tag="PKZ74_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91894.1"
                     /translation="MRNIKWLDLPPCFVLLLFHQVSDKRLAKAGRMETTLSPQGGGRV
                     QSPPRTPEAERRRGARGSKDRTTPKGSAREGRPPKPGATEAKSAERRTRGREPDKEEK
                     EPKGTGTKG"
     gene            complement(3713..5650)
                     /gene="dnaG"
                     /locus_tag="PKZ74_03720"
     CDS             complement(3713..5650)
                     /gene="dnaG"
                     /locus_tag="PKZ74_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPM91895.1"
                     /translation="MISKQTIARILEDARIEEVVGEFVKLKKSGQNYKGLSPFTAERN
                     PSFYVSPAKGIFKCFSSGKGGNVVNFLMEHEHFTYPEALTYLAKKYGIAIEEDSITPE
                     DVQIENERETLFQVTAFAMNFFMQNLHETEAGKAIGLEYFKSRDLKPDTIKKFQLGYS
                     PESWDAFTKNALENGYKLEYLEKTGLTITKDGRSYDRFRGRVIFPIHNITGRILGFGG
                     RTLLKDDHVPKYVNSIESEIYSKSQTLYGLYFAKSAIIRLDNCYLVEGYTDVISMHQA
                     GIENVVASSGTALTEEQIRLIKRYTPNITILYDGDPAGIKASFRGIDMVLEQGMNVRI
                     VLFPDGEDPDSYARKHRRAELEELITGGAQDFIRFKTSLLLKEAANDPIKRAGLIKEI
                     VGSISLIPDPIYRSVYIKECSTLMDVQEQMLMSSLNKILYQKNRKQAEQEAARQEVEI
                     PEMPAPKQVEQVNQEIESQEYHLIRLLLLYSNYDIEINEQDELAQKISVARYITEEVN
                     KDDLKFANPVYQKIFDLYNLALLEGNEPDEKLLINDGQDQELISEVITILSSRYELSE
                     NWLKKRILVNSEKEKLLFAVESTLLSFKEKTIDRMINEILQEIKVTASEEDIMLLQTR
                     HKNYKEISIKINQKLGRIITK"
     gene            5849..6601
                     /locus_tag="PKZ74_03725"
     CDS             5849..6601
                     /locus_tag="PKZ74_03725"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPM91896.1"
                     /translation="MTENNLIIPQSQEQLDKTQKARLLMDFFHRIMIHHAMWFAEVQH
                     QYGLDIALDAMKEAYAKSSGIQLNRLAKTLGFEMHDGIPGPLLDLPAEKLDELIESVA
                     VNWLANDGVWFQAVEFKHGLLDAKRCNDTCWAHFSPFEAWSIRNFLNLPENPGLEGLK
                     TALQFRLYATINKQSIVEETPESFVFRMNECRVQNARKRKGLDDYPCKSGGLVEYTTF
                     ARAIDPRIRTECISCPPDPHPGEYYCAWRFSM"
     gene            6801..10166
                     /gene="ileS"
                     /locus_tag="PKZ74_03730"
     CDS             6801..10166
                     /gene="ileS"
                     /locus_tag="PKZ74_03730"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPM91897.1"
                     /translation="MKRKYREYKELNLTRIGKEINAYWKQHATFEKSMHIRDGHREYV
                     FYEGPPSANGKPGIHHVMARAIKDIFCRYKTLKGYLVERKAGWDTHGLPVEIGVEQTL
                     GITKEDIGKKISVKDYNRTCKQEVMKYKDLWDDLTIKMGYWVDLNDPYITFENKYIES
                     VWWLLKKLYEKGLLYEGYTIQPYSPAAGTGLSSHELNLPGAYKEVKDLTVTAQFKVVR
                     NADSEFLFENAFGDVFFLAWTTTPWTLPSNTALAVGKEIDYVRVKSYNPYTNKPITVI
                     LAKDLVNNFFPEKNAELRLEDYQEGQKVIPFRIDGEFKGARLAGMRYEQLLPYARPET
                     GDAFVVVTGDFVTTEDGAGIVHIAPSFGADDFKVARENGIGALTMVDKSGKFVESMGE
                     FAGRYVKPEYDPNFNKGVDESVDVDIVVKLKQENRAFKTEKYVHSYPHCWRTDKPILY
                     YPLDSWFIRTTAFKEQMIELNKTINWKPVSTGIGRFGNWLENLVDWNLSRSRYWGTPL
                     PIWVTEDRSEQKCIGSLEELKGEIGKSVKAGFMKADPLEKFVPGDNSAENYNTFDLHR
                     PYVDEIILVSESGKPMQRETDLIDVWFDSGAMPYAQFHYPFENGQLEHYFPADFIAEG
                     VDQTRGWFFTLHAIAVMLFDSVSFKTCVSNGLVLDKDGNKMSKRLGNAVDPFETLEKF
                     GPDATRWYMMTNSQPWDNLKFDLEGIAEVQRKFFGTLHNTYAFFALYANIDGFHYAEA
                     EVPFEKRPEIDRWILSELNSLIRKVESYYDDYEPTKAGRAIQDFVIDHLSNWYVRLSR
                     RRFWKGDYTEDKIAAYQTLYRCLEVVAQLASPIAPFFMERLFTDLNEVSGRIGSDSVH
                     LTDFPSANESQIDLDLEERMELAQKISSMILSLRKRSNIRVRQPLNKIMIPILNEHNK
                     KQLQAVENLILSEVNVKEIEYLTETAGILVKKIKPNFKTLGPRFGKLMKPIAAAISSL
                     NPEEINKMEEEECLKMTVEGEPIEITIHDVEITTEDIPGWLISSAGNLTVALDINITD
                     ALKKEGLARELINRIQNIRKERDYEVTDKIRVQVVRKDYLDEAIYHNLSYICAEILAE
                     SLVLVEKTEGDNPVEVELTEEFNTIIRVEKINS"
     gene            10200..10607
                     /locus_tag="PKZ74_03735"
     CDS             10200..10607
                     /locus_tag="PKZ74_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPM91898.1"
                     /translation="MTDIKKQKADTQEKNRYSDEELEEFKQIILDKLEKANEDLRLLM
                     EAYHLDGEHGTDDTSPTFKILEEGYQVMSKEENSRLASRQQKFINNLENALLRIENKT
                     YGICRVTGKLISKERLKSVPHATLSIEAKLNQR"
     gene            10611..11240
                     /locus_tag="PKZ74_03740"
     CDS             10611..11240
                     /locus_tag="PKZ74_03740"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPM91899.1"
                     /translation="MKKAILIVFLVLVADQVLKFWIKTHMTIGEEIHVLGNWFILHFT
                     ENEGMAFGITFGGAFGKLILSIFRIIAVIVIAIYMFRMRAKSPHPGLVWSFSLILAGA
                     LGNILDSAFYGLIFSHSSYHTVASLFPPGGGYASFLHGKVVDMLYFPVIDTTLPDWIP
                     IRGGTQFIFFRPVFNIADASITTGVLMLLVFQKKFFPKKEGPVQEVIEN"
     gene            11305..12132
                     /locus_tag="PKZ74_03745"
     CDS             11305..12132
                     /locus_tag="PKZ74_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPM91900.1"
                     /translation="MEKKRILFVSQEITPYTEETPIGLKGRYLPQGIQEKGREIRTFM
                     PRYGNINERRNQLHEVIRLSGMNLIIDNNDHPLIIKVASIQQARMQIYFIDNEDYFQR
                     KAQLYDKSGKFFEDNDERAIFFARGVLETVKKLGWAPDIIHCHGWFTGFVPLYLKKSY
                     RDNPLFSETKVVLSIYNDDFKEDFPADISKKIKYEGITKQDLAYYKKTHYVSLLKAAM
                     EYSDGIIAGSPEINTELDSHAKASGKPYLEYQEEDKYIDAFSDFYDKVLNEETGKAK"
     gene            12172..13500
                     /locus_tag="PKZ74_03750"
     CDS             12172..13500
                     /locus_tag="PKZ74_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HPM91901.1"
                     /translation="MIKKLSWLFAGIILVMVFNMAGCKQDDNDLGIDIQPPNDKLNVF
                     SSDTATILAYSTLVDSVKTDETSVTLLGSLVDPVFGRSTASFYTQLRLSKTAYDFGST
                     PYPDSIVLTLDYDDIYGDTLAPMTIRVYEMSERIFIDSMYYSNHSVAYESDLLAEKTF
                     TPDLSTDVIIGEDTLDPHLRINLGQFSFSLAEKLMNAPADSMASNASFINYFYGLYVT
                     AEPATAGGSIVYFNLLSELSEMTLYYHNGEDDSLRFSYLINSNCARFSHFDHDYSLGS
                     PEFKAQVIDGDTTLGKSVCYVQALGGVKTYIRFPNIKNYYNNGKIAVNEARFFLQAYE
                     QNPELARAASLIMVKRNGDGSYSILNDQLDGDSYFGGFYDESKGGYWFRITSTVQELM
                     RSDSTDYGYEIYLSGGSINSERVLLSGTNPQSPVDAEDRMKLVITYTTLN"
     gene            13511..>14999
                     /gene="glmS"
                     /locus_tag="PKZ74_03755"
     CDS             13511..>14999
                     /gene="glmS"
                     /locus_tag="PKZ74_03755"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPM91902.1"
                     /translation="MCGIVAYLGPQEAYPILMKGLYRLEYRGYDSAGIAILNDELSVL
                     KTKGKVADLETLTKNMALEGTIGIAHTRWATHGEPNDINAHPHYSQSKNLAIIHNGII
                     ENYLTLKKELINRGYKFTSNTDTEALIHLIEDIQLNEKVDLVEAVRIALNQVVGAYAI
                     VIISKNHPDMLIAARKSSPLVVGIGAEELFIASDATPIVEYTKDVVYLNDEELAILRR
                     GKPLKIITIKNKEKTPYIQKLELNLSALEKSGFEHFMLKEIFEQPRSIRDSLRGRLRL
                     QEGTVSLGGIVDYEQKLMNAERIIIVACGTSWHAGLVGEYLLEELARIPVEVEYASEF
                     RYRNPILCEKDVVIAISQSGETADTLAAIELAKGRGATILGICNVVGSSIARATHAGS
                     YTHAGPEIGVASTKAFTAQVAVLAMMALTIASKKGTIPKSRFLRIIHELDLIPEKVEK
                     TLQVDPLVHSIAEMYKDHRNFLYLGRGYNFPVALEGALKLKEISYI"
BASE COUNT         4022 a         3768 c         3713 g         3496 t
ORIGIN      
        1 gtcagggata cactcaagta tggttttatc gatgagaaga tcaatggtaa acagggaatg
       61 cgcctcctgg tcatgaaaca atcaggagcc aataccgtaa gtatcgcccg ggatgttcac
      121 gcgaaaatgg atgagttggt aaaaactttg ccgcccgaca tcagtgtaaa cccgatattc
      181 gacagctccg actttatcaa aggatccatc gacaacctgt cggaaaccct gatgtgggct
      241 tttcttttcg ttatcctggt cgtccttttc ttcctcggcc gctggagggc caccatgatc
      301 attatcctga ccatcccgat ctcgctggtg gtctccttca tctacctcta ttttaccggc
      361 agttcgctga acgtcatctc cctttcatcc ttatccatag ctattgggat ggtagttgac
      421 gacgccatag tggtgcttga aaatattaca aaacacatcg aaaggggatc cagcccgcgg
      481 gaagccgcta tttacgccac aaacgaagtg tggctggccg taattattac cacccttgtc
      541 atcgttgccg tattccttcc cctgaccatg gtcggcggca tgacgggcgt gatgttcaac
      601 gaactgggat ggatcgtcac cattaccatt gtgacctcaa cccttgtggc cattacgcta
      661 actcccatgc tctcttccaa gttgctgaaa acatggatac cgaaagaaaa gtcaatgaat
      721 tcagcttatg ataaaaccat ccgaaagggt ctcgacaaac tggataattt ttatgagaga
      781 accatccgtt gggccctgca ccacaaaagc ctggtcatta tcgttagcct ggccatattt
      841 atcgggagcc ttctactggg tgcgtttttt gtaaaaaccg attttatgcc ggaaagtgac
      901 gagagcagga tgaccgtctc tatcgaactg caaacaggct tgagaatgga agaatcgaag
      961 atcacggcag aaaaggtcga gaagatcatc cgggagcgtt atcccgaaat cacgatcatt
     1021 tcgaattcga ccggcgccga cgacgaaggt aatttctttt cgctcttcag acccaccgga
     1081 tcgaaccgga tcaacctgat gatgcggttg gtgcccgtta ccgagcgcag ccgttccgtc
     1141 tttgaaattg cggacgactt ccgcaaccag ctcgatcaga ttgccgaggt ggtcaattac
     1201 gatattgcca catcgaacaa catgatgggc ggcggatcca ataccgttga tgtggaaatc
     1261 ttcgggtacg atttcaatga aacgaaccgg attgccgatg agatcaagac acggatcagc
     1321 cggatacccg gcgccaaaga cgtttcgttg agccgggaga aggaccggcc ggaactgcag
     1381 atcgtgctcg acagggaaaa actggccatg aacggattga acagcgcgct ggtgtcgacc
     1441 tatatccgga accgggtaga tgggatgacc gcttccaaat tcagggaaga aggcgatgaa
     1501 tacaatattg agctgcggta ccaggaagat tacaggaatt caatcaccga cctggaagag
     1561 atcaccatca taaatccatc gggacaacgg ataaagctta aagaactggg agaagtgaag
     1621 gagtactggt cgccgccgaa tatcgagcat aaacgccggg aaaggatcgc caaagtatcc
     1681 gtcaaaccgg tcggggtgtc gttgggagag ctggccgaaa gtatcaggaa agaagttgct
     1741 tcggtcgaag ttccgccgga gatcatgatc aacgtaggag gcgcctatga agaccagatg
     1801 gaaggattcc aggatatcgg tttgctgatg atcatgagtc ttgtgctggt atttattgtc
     1861 atggcgtcgc aatttgaatc gtttgccatg ccatttgtga tcatgttctc cattcctttt
     1921 gccttcacgg gcgtagtgct gagcctgttc ctgaccaaca ccaccctcag cctgattgct
     1981 gccctggggg ctgtcttgct gatcgggatt gtggtgaaga acggcattgt gctggtggat
     2041 ttcatcaacc ttatgcgcga caggggattt gagttatacg aagcgattgc ccgggccggg
     2101 cattcccggt tgcgcccggt attgatgact gccggcacca ctatccttgg gatgcttccc
     2161 ttggcgctga gcaagggtga aggatcggag atctggaccc cgatggggat caccgtgatc
     2221 gggggactgg tcatttccac gattgttacg atggtgatcg tcccggtcat gtacgccata
     2281 cttgcccgac ggggtgagcg tgataaaaag accaggctaa ggaagcggtt tttattcatg
     2341 gacaaataag aaatagaaag atgaaagcgg tatttatcgt atttaaccag gcccataccg
     2401 agagggtgga atacttgttc gacaggctgg agattcgcgg atacacctgg tggaccgatg
     2461 tccatggaag gggatcggcg tcaggagagc cgaggatggg cacccatacc tggccggaaa
     2521 tcaattcggc agctatggcc attgtggatg acaatgcagt agatgagatc ctggaaaaag
     2581 tcaggaggat cgatgaaatc aaccgggaag tgggaattcg tgcctttgta tgggatattc
     2641 tgaaatcggt ctgatcattc gggaaacaaa acgattgata ttgtttcatc taattaacaa
     2701 ttgttaataa cgggtgtgat taacttgtgg attgtgaaac ataaaatggc ttgacttacc
     2761 cccctgtttc gttttacttt tgtttcatca ggtcagcgat aaaagactgg caaaggcagg
     2821 caggatggag acgacgctga gtccgcaagg aggaggcaga gttcaaagtc cgccccggac
     2881 accggaggcc gaaaggcgga ggggagcgag ggggagcaaa gaccggacaa cgccgaaagg
     2941 cagcgcgagg gaaggccggc cgccaaagcc gggagccacg gaagctaaat ccgccgaaag
     3001 acggacacgc ggaagggaac ccgacaaaga ggagaaggaa ccgaaaggaa ccggaaccaa
     3061 aggttagtca aaataacagt agcctgacgc aaaccgcaag cttcggcaaa cggcaagcga
     3121 acggggatcg atccggcgag gttcggtccc cgttgtgttt ttaatcagtt taagatacta
     3181 acaattgtta ataatagttg tgagaaactt gtggattgag aaacattaaa tggcttgact
     3241 tgcccccctg tttcgtttta cttttgtttc atcaggtcag cgataaaaga ctggcaaagg
     3301 caggcaggat ggagacgacg ctgagtccgc aaggaggagg cagagttcaa agtccgcccc
     3361 ggacaccgga ggccgaaagg cggaggggag cgagggggag caaagaccgg acaacgccga
     3421 aaggcagcgc gagggaaggc cggccgccaa agccgggagc cacggaagct aaatccgccg
     3481 aaagacggac acgcggaagg gaacccgaca aagaggagaa ggaaccgaaa ggaaccggaa
     3541 ccaaaggtta gtcaaaataa cagtagcctg acgcaaaccg cacgcttcgg caaacggcaa
     3601 gcgaacgggg atcgatccgg caaggttcgg tccccgttgt gttattgata atatcctccg
     3661 tttaattgta taaaatccac gtagtggata cagaacggaa gacccgcttt gattatttag
     3721 tgattattct gcctaatttt tgattaattt ttatactgat ttctttataa ttcttatgcc
     3781 tggtctgtaa aagcattata tcttcctcac ttgctgtgac ttttatttct tgcagaattt
     3841 cattgatcat cctgtctatt gtcttttctt taaacgaaag taaagttgac tcaaccgcaa
     3901 aaagcagctt ctctttttca gagttaacta aaattctttt ttttaaccaa ttttcgctca
     3961 actcataccg actgcttaaa atggtaatga cctcactaat aagttcttga tcctggccgt
     4021 cattgattaa caatttttca tcaggctcat tgccttcaag caacgccaaa ttatacaaat
     4081 caaaaatctt ttgatatact ggatttgcaa atttcaaatc atccttgttg acctcttcag
     4141 taatatatct tgccacactg atcttttgag ccaattcatc ctgttcatta atctcaatat
     4201 cataattgga atataataat aacaatctga ttaaatgata ctcctgggat tctatttcct
     4261 gattcacctg ttccacctgc ttcggcgccg gcatctccgg aatctccacc tcctgcctcg
     4321 ccgcctcctg ctccgcctgc ttcctgttct tctggtataa tatcttgttc agcgacgaca
     4381 tgagcatctg ctcctgcaca tccatcagcg tactgcactc cttgatgtag accgaacggt
     4441 aaatcggatc gggtatcagc gatatgctcc cgacaatctc tttgatcaat ccggcccgct
     4501 tgatgggatc attggccgct tctttcagca acaggctggt cttgaaccgg ataaaatcct
     4561 gcgccccgcc ggtgatcagc tcctccagct cggcccgccg gtgcttccgg gcataagaat
     4621 cgggatcttc tccatccgga aataaaacga tcctgacatt cattccctgc tccagcacca
     4681 tgtcgatccc ccggaatgaa gccttgatcc ccgccggatc accgtcgtac aaaatcgtga
     4741 tattcggcgt gtaacgcttg atcagccgta tctgctcctc cgtgagcgcc gtgcccgatg
     4801 aagccaccac gttttcaatc ccggcctggt gcatcgaaat cacatcggta taaccctcca
     4861 ccaggtaaca gttgtcgagc cggataatgg cgctcttcgc aaaataaagg ccgtaaaggg
     4921 tctggctctt gctgtatatc tccgattcga tcgaattgac atacttcggg acgtggtcgt
     4981 ccttcagcaa ggtccgcccg ccaaaaccca gaatccgacc ggttatgttg tggatcggga
     5041 aaatgacccg gcccctgaaa cggtcataac tccggccgtc tttcgtgatc gtcagccctg
     5101 tcttttccag gtactccagt ttgtagccgt tctccagggc gttcttcgtg aatgcatccc
     5161 agctctctgg actgtaaccc agctggaatt ttttgatcgt gtcgggcttc aggtcgcggc
     5221 tcttgaaata ttcaagcccg atggctttgc cggcttccgt ctcatgcagg ttttgcatga
     5281 agaaattcat ggcaaacgcc gtcacctgga aaagcgtttc ccgttcattt tcaatctgaa
     5341 catcttcagg agtaatcgaa tcctcctcga tggctatgcc gtactttttc gccaggtagg
     5401 tcagtgcttc gggataggtg aaatgttcgt gttccatcag gaaattcacc acatttcccc
     5461 ccttacccga actgaaacat ttgaagatcc ccttggccgg cgacacatag aacgaagggt
     5521 tgcgttcggc cgtaaacgga ctcagcccct tgtaattctg cccgcttttt ttgagtttga
     5581 caaattcgcc gacaacctct tcaatacggg cgtcttccag aatacgcgct atggtttgtt
     5641 tggagatcaa ggttattaat atgctttaca aagataataa tgcattagag ggaaggggaa
     5701 attgttgata atatcttgag atggcatctc aaatcgatcg tccaatcttc tgtttcgctt
     5761 gccagataaa ccattcctga ttttaatcag atattctgag atgccatctc aaaaaacaat
     5821 aaattagccc cttaattata acctacccat gacagaaaac aatctcataa tcccccaatc
     5881 gcaggaacag cttgacaaaa cacagaaagc ccgcctgctg atggactttt tccaccgtat
     5941 catgatccac catgccatgt ggtttgccga agtgcagcac caatacggtc tggatatcgc
     6001 ccttgatgcc atgaaagagg cgtatgccaa aagttccggc atccagctga accggctggc
     6061 gaaaacgctg ggatttgaga tgcatgacgg aattcccggc ccgctcctcg acttgcccgc
     6121 tgaaaaactc gacgaactga tcgaaagcgt ggcggtcaac tggctggcca acgacggtgt
     6181 ctggttccag gcggtcgagt tcaaacacgg cctgctcgac gccaagcgct gcaacgatac
     6241 ctgctgggca cacttctcgc cgttcgaagc ctggtcgatc cgtaatttcc tcaatctgcc
     6301 ggaaaatccg ggcctggaag ggcttaagac cgcgcttcaa ttcaggctat atgccaccat
     6361 caataaacag tcaatcgtcg aagaaacccc cgaaagtttc gtcttccgga tgaacgaatg
     6421 ccgcgttcag aatgcccgta aacggaaagg gctggatgat tatccctgca aatcaggggg
     6481 cctcgttgaa tacaccactt tcgcccgggc catcgacccg aggatcagga ccgaatgcat
     6541 ctcctgcccg ccggatccgc accccgggga gtattactgc gcgtggaggt ttagtatgta
     6601 gtcactggtc atgagctcgc ttcgctctcg tcattgaggg tcataaagtt gtgttaaatt
     6661 agtcataagg tcgtcataag gtggtttaag gtttattgat tgagatttga catttgagtt
     6721 ttgtttgggt ttcggaattt ggaatttgga atttccgtac ctttgcgcct catttttaat
     6781 tttttgcaac taaaggctcc atgaaacgca aatacaggga atacaaggaa ctgaacctga
     6841 cccgcatcgg caaggagatc aatgcgtact ggaaacagca cgcgactttt gagaaaagca
     6901 tgcacatcag ggacggacac cgggagtatg tattttacga agggccgccg tcggctaacg
     6961 gaaaaccggg tatccaccac gtgatggccc gggcgatcaa agacattttc tgccgctata
     7021 aaacgctgaa aggttacctc gtcgagagaa aagcaggctg ggatacgcat ggacttccgg
     7081 ttgaaatagg cgtggaacag acgctgggaa ttaccaaaga agatatcgga aagaaaattt
     7141 ccgttaaaga ttataaccgt acctgcaaac aggaggtgat gaaatacaag gacctgtggg
     7201 acgacctgac cattaaaatg ggttactggg tcgacctgaa cgatccgtat atcacgtttg
     7261 aaaacaaata tatcgaatcg gtctggtggc tgcttaagaa actttacgaa aaggggcttc
     7321 tttacgaggg atacacgatc cagccctatt cgccggcagc aggaacaggg ctcagctcgc
     7381 atgaactgaa cctcccgggt gcctacaagg aggtcaaaga ccttacggtt accgcccagt
     7441 tcaaggttgt cagaaatgca gactcggaat tccttttcga aaatgcattt ggcgatgtgt
     7501 ttttcctggc ctggacaacc acgccctgga cacttccgtc gaataccgca ctggctgtcg
     7561 ggaaagaaat cgattatgtc agggtgaaat cctataatcc ttatacgaat aaacccatca
     7621 ctgtcatcct ggccaaagac ctggtaaaca acttctttcc ggagaaaaat gcagagctga
     7681 gactggaaga ttaccaggaa gggcaaaagg tcatcccttt ccggatcgat ggagaattta
     7741 aaggagcccg gctggccggt atgcgctatg agcaacttct tccctatgcc cggcccgaga
     7801 caggcgatgc cttcgttgtg gtgaccggtg attttgtgac tacggaagac ggcgccggca
     7861 tcgtgcatat cgccccgagc tttggcgccg acgacttcaa agtggccagg gaaaacggta
     7921 tcggcgcact gacgatggtc gacaaatcgg gaaaattcgt cgaatcgatg ggcgaattcg
     7981 ccggccgcta tgtcaaaccc gaatacgacc cgaacttcaa caaaggggtc gatgaaagcg
     8041 tggatgtcga tatcgtggtg aaactcaagc aggaaaacag ggccttcaaa accgagaagt
     8101 acgttcacag ctatccgcat tgctggcgca ccgataaacc gatcctctat tatccgctcg
     8161 actcgtggtt tatccggacc acagccttta aagagcagat gatcgagctg aacaaaacga
     8221 tcaactggaa accggtgtcg accggtatcg gccggttcgg caactggctc gaaaacctgg
     8281 tcgactggaa cctttcccgt tcccgctact ggggaacgcc gctgccgatc tgggtgacgg
     8341 aagaccgcag cgagcagaag tgcatcggct cgttggagga attgaaaggg gaaattggaa
     8401 aatcggtaaa ggcagggttc atgaaagccg atccgctgga gaagttcgtt cccggcgaca
     8461 attcggccga aaattacaac accttcgacc tgcaccggcc ttacgtcgat gagatcatcc
     8521 tggtatcgga atcgggtaaa ccgatgcaga gggaaaccga cctgatcgac gtgtggttcg
     8581 attcgggcgc catgccgtat gcccagttcc actatccgtt tgagaacggg cagctggagc
     8641 attatttccc ggccgacttc atcgccgaag gggtcgacca gacccggggt tggttcttta
     8701 cgctgcatgc cattgccgtc atgcttttcg actccgtatc cttcaaaacc tgcgtctcca
     8761 acggcctggt gctcgacaag gatggcaaca agatgtcgaa acggctgggc aatgccgttg
     8821 atccctttga aacgctggaa aaattcggcc ccgatgccac gagatggtac atgatgacca
     8881 actcacagcc gtgggacaat ttgaagttcg acctggaggg gatcgcggag gtgcagcgca
     8941 aatttttcgg caccctccac aacacatacg ctttctttgc cctctatgcc aacatcgacg
     9001 gattccatta tgctgaggca gaggtgccgt ttgaaaagcg gccggagatc gaccggtgga
     9061 tcctttcgga gctcaattcc ctgatccgca aagtagagag ctattacgac gactatgaac
     9121 cgaccaaggc cggtcgcgcc atacaggatt ttgtgatcga ccacctgagc aactggtatg
     9181 tgcggttgtc gaggcgccgg ttctggaaag gcgattacac cgaagacaag attgcggcat
     9241 accagacgct ttaccgttgc ctggaggtag tggcacagct cgcctcgccc atcgccccct
     9301 tcttcatgga gcgcctcttt accgatctga acgaggtgtc ggggaggatc gggtccgaca
     9361 gcgttcacct gaccgatttt ccttcggcca atgaaagcca gatcgacctt gaccttgaag
     9421 aacggatgga actggcccag aaaatctctt ccatgatcct gtcgcttcgc aagcgttcga
     9481 atatccgcgt ccgccagccg ctgaacaaga tcatgatccc gatcctgaac gagcacaata
     9541 aaaagcagct tcaggctgtg gaaaacctga tcttatcgga agtgaatgtc aaagagatag
     9601 aatacctgac cgaaacagcc ggcattctcg ttaagaaaat caagccgaat ttcaaaactt
     9661 taggaccacg gttcgggaaa ctgatgaaac cgatcgccgc agccatctcc tcactcaacc
     9721 cggaagagat caataaaatg gaagaggaag agtgcctgaa gatgacggta gaaggagaac
     9781 cgatcgagat cacgatccat gacgtcgaaa tcactacaga agacatcccg ggctggctga
     9841 tctcctcggc cggcaacctg acggtagccc tggatatcaa catcaccgat gcgctgaaaa
     9901 aggaaggatt ggccagggag ctgatcaacc gcatccagaa catccggaag gagcgtgatt
     9961 acgaagtgac cgacaagatc agggtgcagg tagtgcgtaa ggattatctc gacgaggcca
    10021 tatatcacaa tttatcttat atttgcgccg aaattctagc cgaatcactt gttttggttg
    10081 agaaaaccga aggagataac ccggtggaag ttgaactgac cgaagagttc aacacaatca
    10141 tcagggtgga aaaaattaat tcataatcaa taatttttta acggattaaa tcccgggcca
    10201 tgacagacat taagaaacag aaagccgata ctcaggaaaa gaacaggtat tctgacgaag
    10261 aactggagga attcaaacag atcatcctgg ataaactgga aaaggccaac gaagacctca
    10321 ggttgctaat ggaggcgtat catcttgacg gagaacatgg tacagatgat acatctccta
    10381 ctttcaagat cctggaagaa ggataccagg tcatgtcgaa agaagagaac agccggctgg
    10441 cctcacgcca gcaaaagttt atcaacaacc tggaaaatgc cttgcttcgc atcgagaaca
    10501 agacctacgg catctgcagg gtgacaggca agctcatttc gaaagaacgg ctcaagagcg
    10561 taccgcacgc cacactcagc atcgaagcca agcttaacca gagataggcc ttgaagaaag
    10621 caatcctgat tgtttttctg gtgctggtcg ccgaccaggt gctcaaattc tggatcaaaa
    10681 cccatatgac catcggggaa gagatccatg tgctgggcaa ttggtttatt cttcatttta
    10741 ccgaaaatga ggggatggca ttcggcatca cattcggagg cgccttcggg aaactgatcc
    10801 tgagcatatt ccggatcatt gccgttatcg tgatcgccat ttacatgttc cggatgcggg
    10861 ccaaatcgcc ccatcccggg ctggtttgga gcttctcgct catcctggcc ggtgcattgg
    10921 gaaacatcct cgacagcgct ttttacggac tgatcttcag ccatagcagt tatcatacag
    10981 tagcttcgct tttcccaccc ggcggaggat acgcgtcgtt ccttcacgga aaagtggtcg
    11041 atatgctcta ttttcccgtc atcgatacca cattgcccga ttggatcccg atccgtgggg
    11101 ggacgcaatt tatttttttc cgccccgttt tcaacatcgc cgacgcatcc attaccaccg
    11161 gcgttttaat gctgctggtt ttccagaaga aattctttcc caaaaaagaa ggtccagtgc
    11221 aagaggtaat tgaaaattga ttaattactt aattttaact acctttgcac ttcttttttt
    11281 agaaacacac acaagcttcc ggttatggaa aaaaagagga tcctcttcgt atcgcaggaa
    11341 atcaccccat atactgaaga aacacccata gggctcaagg gccgctacct tccacagggg
    11401 atacaggaaa aaggcaggga aatccgcacc ttcatgcccc gttacggcaa catcaatgaa
    11461 cgccgaaacc agcttcacga agtgatccgc ctttcgggca tgaacctcat catcgacaac
    11521 aacgaccacc cgctcatcat caaagtcgcc tccatccagc aagcccggat gcagatctat
    11581 tttatcgaca atgaggacta cttccagcgg aaagcccagc tttacgataa atcggggaag
    11641 tttttcgagg ataatgatga acgggccatc tttttcgcgc gcggcgtgct cgaaacggta
    11701 aaaaagctcg ggtgggcccc cgatatcatc cactgccacg gctggtttac cggatttgtc
    11761 cctctatacc ttaaaaaatc gtaccgcgac aatccccttt tcagtgaaac taaagttgtc
    11821 ctatctattt ataatgatga ctttaaggaa gattttccgg ccgatatctc caaaaaaatt
    11881 aaatatgaag gtataaccaa acaagacctt gcatactaca agaagaccca ttacgtttca
    11941 ttactcaagg cggcaatgga atattccgac ggtatcattg ccgggagccc tgaaattaac
    12001 acagaactgg attcacatgc caaagcttcc ggaaaacctt acctggaata ccaggaagaa
    12061 gataaatata tcgatgcttt ctcagatttt tacgacaaag tactgaacga agagactgga
    12121 aaagcaaagt aacttatcga cgcctcatca ttttacaaac aaaccgcaag aatgataaaa
    12181 aagctgagtt ggttatttgc agggataatc ctggttatgg ttttcaacat ggccggttgt
    12241 aaacaggatg ataatgacct ggggattgat atccagcctc ctaacgacaa gctgaacgta
    12301 ttttcatccg acacggctac cattttagcc tattcgactc tggtcgattc ggtgaaaacc
    12361 gacgaaacat ccgttacact cctcggcagc ctggtcgatc cggtattcgg acgctccacg
    12421 gccagtttct acacacagct caggctaagc aagacggctt acgattttgg ctctacccct
    12481 tatcccgact ctattgtcct taccctcgat tacgatgata tttacggaga tacactggct
    12541 ccgatgacca tccgggttta tgaaatgtcg gagaggatct ttatcgactc catgtactat
    12601 tcgaaccatt ctgtagctta cgagagcgac ctcctggccg aaaagacctt tacccctgac
    12661 ctatccaccg atgtgatcat cggtgaagac acactggatc cgcacctgag gatcaacctg
    12721 gggcagttct cattttcact tgcagaaaaa ctgatgaatg ccccggccga cagcatggcc
    12781 agtaacgcca gcttcatcaa ctatttctat ggactgtacg tcacggccga accggcaacc
    12841 gccggaggtt ccatcgtcta tttcaacctg ttgtcagaac tttccgaaat gacgctgtat
    12901 tatcacaacg gggaagatga ttcactccgg ttttcctacc tgatcaacag caactgtgcc
    12961 cggttcagcc atttcgatca tgattattcg ctggggagcc ctgaatttaa agcgcaggtg
    13021 atcgacggcg ataccaccct gggaaaatcg gtttgttatg ttcaggcttt aggtggcgtt
    13081 aaaacatata tccgttttcc aaatattaaa aattattata acaacggaaa gatagcggtc
    13141 aatgaggccc ggtttttcct ccaggcctat gaacaaaacc ctgaactggc ccgtgccgct
    13201 tcgctgatca tggtgaaacg aaacggcgac ggttcctata gtattttaaa cgaccagctt
    13261 gacggcgaca gctattttgg cggattttac gatgaatcca aaggcggata ctggttccgg
    13321 atcacatcga ccgtccagga actgatgcgg agcgacagta ccgattacgg ctacgaaata
    13381 tacctgagcg gcggatcgat caattcggaa cgtgtgttgc taagcggaac aaatccccag
    13441 tctccggttg atgccgagga ccgtatgaag ctggtcatta cttatacaac cttaaactga
    13501 aaaccaatct atgtgcggaa ttgttgctta ccttggccca caggaagctt acccgatcct
    13561 gatgaaaggc ctgtaccggc tcgaatacag gggctacgac agcgccggga tcgccattct
    13621 taacgatgaa cttagcgttc tgaaaaccaa aggaaaagtc gctgatcttg agaccctgac
    13681 aaaaaatatg gcgttggaag gaacgatcgg aatagcccac acacgatggg caacccatgg
    13741 cgaacccaat gatatcaacg cccatcccca ttattcccaa tccaaaaacc tggcgatcat
    13801 ccataacggt atcatcgaaa attatcttac cctgaaaaaa gagctgatca accggggata
    13861 taagtttacc agcaataccg acaccgaagc cctcattcac ctgatcgaag acattcagct
    13921 caacgaaaag gtcgacctgg tcgaagcggt gcggattgcc ctgaaccagg ttgtgggcgc
    13981 ttatgccata gtgatcatct caaagaatca ccccgacatg ctgatcgccg cccgcaaaag
    14041 ttccccgctg gtggtcggga tcggggctga ggaactgttc atcgcgtccg acgcaacccc
    14101 aatcgtagag tacacaaaag acgtcgttta cctgaacgac gaggaactgg ccattctccg
    14161 cagaggaaag ccgctgaaga tcatcactat aaagaacaaa gaaaagacac cctacattca
    14221 gaaactcgag ctgaatcttt ctgccctgga gaaaagcggt tttgagcatt tcatgctgaa
    14281 agagatattc gagcagcccc gctccatccg cgacagtctg cgcggcaggc tcaggttgca
    14341 ggaagggacc gtgtcactcg gcgggattgt cgactatgag caaaagctga tgaatgcgga
    14401 acggatcatc atcgtagcct gtggtacatc ctggcatgcc ggtcttgtcg gcgaatatct
    14461 gctcgaggag ctggccagga tacccgtaga agtcgaatat gcctccgaat ttcgttaccg
    14521 caatcctatc ctgtgcgaaa aagatgttgt gatcgccatc tcgcaatcag gtgaaacggc
    14581 ggatacgctg gccgccattg agctggccaa agggagaggc gccacgatcc tgggaatctg
    14641 taatgtggtc ggttcctcga tcgcccgggc tacccatgcg ggctcctaca cccatgctgg
    14701 cccggagatc ggcgtagcct ccaccaaagc attcacggca caggtagccg tactggccat
    14761 gatggcgctg accattgcct cgaaaaaagg aacgatcccg aaatcccgct ttctccgtat
    14821 catacacgaa ctcgacctga tcccggaaaa agtggagaag accctacagg tcgacccgct
    14881 cgtccattcg atcgcggaga tgtacaaaga ccatcgcaac ttcctttacc tggggagagg
    14941 ctataatttc ccggtggcgc tggaaggggc cctgaagctg aaagagattt cctacatcc
//
LOCUS       DAOPNH010000044        14975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_3999_length_14975_cov_5.616823, whole genome shotgun sequence.
ACCESSION   DAOPNH010000044 DAOPNH010000000
VERSION     DAOPNH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3999_length_14975_cov_5.616823"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PKZ74_03760"
     CDS             <1..1699
                     /locus_tag="PKZ74_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91903.1"
                     /translation="ITYTTNGSNPQTVGANSSPYILPVYPAVPTTYEFTAVEDIYCSG
                     LASGVVEADVDPSPTANAGNDKTIPYGTSTTLNGQASGGSGNYQYSWQPAAKLTNPTA
                     QNPLTVNLTESTLFTLTATDNVGGCYDLDDIMVSLSGGPLGCIPVADPPAVCTGDPSH
                     LLPYVSGGSGTYTYLWSSNPPGFSSYLPDPVVTPAQTTTYSVQINDGYNVISGNVTVT
                     VHPSPIPDAGEDVTIPHGTNTILHGSAGNGSGNYAYHWEPADLLVDPNVASPQTENIY
                     GTTTFSLFVTDLTTSCESQSPDVMTLIISGGPLAVVPAAYPETVCLGETVKLYANAGG
                     GAGAENYQYSWLGSDGFNSTLQNPEVTPNQVGTIYYTCTLDDSFNTTFGSVAVNVLTT
                     PEIDFGFTSKTVCVFDTVILDPDVPNASYLWSNGATTQTIRAASTGIGFDMQTYTVTV
                     TSLSGGCQSEQSVNVIFDFTACTGVGEDLSSAICQIYPNPGDGNIRMIFDPELKQAEI
                     DVLSIQGYPVSDVFTYYKSLMNNELILPVGYLSEGMYFIRISDKKGINQLYKYILRK"
     gene            1811..3793
                     /locus_tag="PKZ74_03765"
     CDS             1811..3793
                     /locus_tag="PKZ74_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM91904.1"
                     /translation="MNKLVYKNLCFSLPMLFAAAILLIINQSANSQGILKAELRSSNC
                     PVADHEADRWYFGQNAALDFRNQDPFADQTNAMLNVPTCPAVVSDSNGTILIYTDGLN
                     VYNRYNSLVANGSGLHGFVNGAQPVLIVPHPGNDSIYFIFTAGRPKMNPLDPTMIYGL
                     EYSAVNVVRNGFSGEVTKKNHVLLGPEVSTKLTAVRHSNGVDYWVVAHKFNSDEFCSF
                     LVSSNGVDTTGYRSSFVGTTHSAPGESNTFIGFMKISPDGTKLATAIHGAKRFEIFDF
                     NASNGAVSNEIVSDPIFDDAYSVEFSPDSRYLYGTATPVNIEPLDPPHSYIYQFDVND
                     GNQIFDPSFYTVIAQDTLGSYFGGIQLGTDGRIYVSRSPNGYFSLSVIQNPKRDGLAC
                     NFMTNALPLAGKRSYYGFPNFMQSYFDLPHFDVENVCFTDTMVFTLQNNSNITNATWQ
                     FDDGGPVTLNAVQPTYTFSGPGTYDVRVTEFNGTDSYGPYTETVVVRELPTLDIGDTV
                     YMYPGSPIILDAGEGYETYEWSTGESSPKIKIYEAGTYTVTVQNELCCFKSDTAEVIY
                     FDVQVPNAFRPGGANPVFKAFASSPDAIVNFKLFIYNRWGQLLYEGNSLDQGWDGKVK
                     GIDAPGDVYVWLVNYDVERSGKMVTIAYKGNVILLR"
     gene            3795..5930
                     /locus_tag="PKZ74_03770"
     CDS             3795..5930
                     /locus_tag="PKZ74_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPM91905.1"
                     /translation="MDKKYYIRIAEELNISTEQVINTAALLDSGATVPFIARYRKELT
                     GSLDEVVITDVRDRLKQLLDLDKRRDAILKSIEEQGKLTDELRGRILEVMTMAELEDL
                     YLPYKPKKRTRATIAREKGLEPLAKIIMSRFEQDPYRRAKQYVNAQKGVADEEEALAG
                     ARDIIAEWISESQPARARVRKLFLRDGQIYSRIVKGKEIEGVNYRNYYEWNEPVSKIP
                     SHRFLAMMRGEAEGFLKTDVYPPKEKAIETLEDIFIRNATDGCAEQIRKAVNDSYGRL
                     LQPSMENEIKAAAKEKADKYAITVFADNLKQLLMQPPLGQKNVLAVDPGFRTGCKLVC
                     LDKQGNLLHNETIYPHPPENQVKMAEKKILSLVDAYKIEAIAIGNGTAGRETERFIRN
                     IRFDKDLIAVMVNESGASIYSASKIARDEFPDYDVTVRGAVSIGRRLMDPLAELVKID
                     PKSIGVGQYQHDVNQSDLQQSLEDTVTICVNQVGVEINTASKELLSYVSGVGPSLAQS
                     IVNFRKENGPFNSRDQFRKVPRFGDKAFEQAAGFLRIRDAVNPLDRSAVHPESYHVVA
                     EMAKRTGASVADLIQNDDIRKKINLNDYVSEKIGLPTLTDILQELAKPGRDPRQKFDM
                     FEFAKGINSIDDLVPGMVLPGIITNITAFGAFVDIGVHQDGLVHVSQMANRFVRDPKE
                     VVKINQKVMVKVLEVDKERKRINLTMKEI"
     gene            6079..6840
                     /locus_tag="PKZ74_03775"
     CDS             6079..6840
                     /locus_tag="PKZ74_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPM91906.1"
                     /translation="MVFWILAGLVFTGCVREEESIELKIALSKGSPENSYANYYNWVK
                     SLDSNAVSLDMYDMPLDSALALFKGCDGLIITGGTDINPAWYDQEEDSLRCMTIDHYR
                     DTLEMRLIDSALAWKMPILGICRGHQMLNVYFGGSLIIDIPSDYDTLVSHRCPDFNTC
                     FHPVTLADGSLIAELAGQTAGTVNSNHHQGIKNLAPALKIVAFTEDNLPEAVEWNEPA
                     GKTFLMGVQWHPERLGNDNPLSKEIGMRFLRECRK"
     gene            complement(6866..7447)
                     /locus_tag="PKZ74_03780"
     CDS             complement(6866..7447)
                     /locus_tag="PKZ74_03780"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPM91907.1"
                     /translation="MREELTKNRCGWISDPLMQEYHDEEWGVPLHDDRKIFEFMVLDA
                     FQAGLSWKTILNKRENFRRAFDNFIPEKIARYDEAKYEELLKDEGIIRNRSKIRATIS
                     NANAFLAVQKEFGTFDRYIWSFVGHTTIRNNWKSMQDIPAKTEVSDRMSKDLADRGFK
                     FVGSTICYAFMQAAGMVNDHITGCFRYKELQDK"
     gene            complement(7452..8168)
                     /locus_tag="PKZ74_03785"
     CDS             complement(7452..8168)
                     /locus_tag="PKZ74_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPM91908.1"
                     /translation="MKLLRYLFIIGLLMASVSSADAQRFLGFAGAGMNLSQVDGDEVV
                     GYNKAGGHLSLGVSLPIRKKWDVTLETVFSQKGAREKAQYIHTITDSTVVPVKTQTYT
                     GEYNLKLNYVEVPLTAHYTDKERYTVGMGFSYGRLVNSKEIEHGGNIAPYSDTVKFRY
                     NDWNVIFDLQFRIWKQLKGNIRFNYSIFPIRERIYKDVIYGIRDPYERKQYNHLFTVR
                     LIYVFNEKYNKNTQPEELTQ"
     gene            complement(8232..9713)
                     /locus_tag="PKZ74_03790"
     CDS             complement(8232..9713)
                     /locus_tag="PKZ74_03790"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPM91909.1"
                     /translation="MKKHLLEIIVFLCGAVVMIFELAGSRVLGPYLGTSIFIWTSLIG
                     IILGSLSLGYWLGGRLADRKPSPGALAWIILAAGLFIGFTTLVKGPFLKFLPEVINGL
                     KWQSVVASVFLFAPASVFLGMVSPYAVRLKIKALQTSGATVGRLYAISTVGSIAGTFA
                     AGFYLIPAIGTTSILIAITILLLLISILLFIISKIPAGIIAAVMLTLVNSYILYRDQS
                     GQRAYVDVDTQYSRVWIYDATDQATGRPIRFMRINNESSSAEFHDSREPVFPYMRFYR
                     LAEHFTPGFRSALILGGAAFSYPKYFLQQYPQATLDVVEIDPELTELARQHFNLRDDP
                     RMRIFHEDARTFLNRSEYRYDVIFGDTYKSLYTLPWHLTTREAAQKTYEMLNEGGCVI
                     LNIISSIGGESSLFLQAETATYRDVFPHVYIFAADNPVDEDVLQGTLLVALKTSSKSG
                     LTSSDPELSAMLKNNVTHLIAAGVPVLTDDWAPVDFYTNKSIR"
     gene            complement(10022..10777)
                     /locus_tag="PKZ74_03795"
     CDS             complement(10022..10777)
                     /locus_tag="PKZ74_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM91910.1"
                     /translation="MTNIYNSGKLKASGKATGTFYFKHFGVKDDRSTMKVGTDAVLLG
                     AAVDVAGAASILDIGTGCGVIALILAQRSDALIEAIDIDEESVKQAKENVVKSPWKER
                     INVINVSLQDYTDVCSKKYDLIVSNPPYFSNSLKSPSQRRNLSRHDHSLGYDDLIRIA
                     SQILNPNGSFWVILPEAKCEEFIQKAGQNNLFPSRLLYIIPKPNRNIQLVIIRFDKCS
                     VVAPVIQTLHIKNENNSYSKEYKNLTGEFYLDF"
     gene            complement(10824..13331)
                     /locus_tag="PKZ74_03800"
     CDS             complement(10824..13331)
                     /locus_tag="PKZ74_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM91911.1"
                     /translation="MTSPAKAFAFLICLVIPVLASGQKLSLEVQGKPASEVLYDFSGR
                     YGLQISFNEKSLSQYKVNITGEFSSPEEAIRKVIENLPFELEKQGDVFIIIPQKKSPK
                     KRTYSITGQVIEKGTREPLPYSHVIINNTGIATDIKGSFSYISSTDSVFQIKATHLGY
                     FILDTLVMGNRNVQFSLSPNFIGLNEVIITHSVVERASLIGREPGVMKLNHQVANFLP
                     GFGDNSVFNLLRLQPGILASGEQTNELIVWGCYEGQSKVIFDGFTLYGLKNFNDNIST
                     FNPLMAKDIEVYKGGFDARYGERVGGIVNITGKNGNTEKTSFTVDINNMTMNGMVEIP
                     LFKTGSLIFALRHTYYELYNPGDVNDLFSRGRGNNAVENVDVNIIPDYRFGDMNLKYS
                     ARIRNTDLFYISLYSGNDRFSYSINEPFNHIIIKKNTSEKNRQSGGSVFYGKSWKNGY
                     STHFSLNYTVLNSTFTDNLEIEKVIQQKTEQRIDLYAFNSIRENSLRVDNRFPVHESH
                     ILEGGMIYMANRIELAEDTFDIPRVRFDEEAKRMTLYFQDNIKLLNIIDIKAGFRLTR
                     ANNLKKFYFEPRLSASVRLGESFKINGAWGLYDQWVTKSSVLDDMGNYRYIWTVCDNE
                     SIPVLKAYHYVLGTSYNHNDLTVSIEGYYRNVSGLTRYIRNTALNIEGIYEGIGRSYG
                     VDLMIKKDYKGHSAWISYTVGKTEELFEYFKKDEYRRAPQDQRHEVKAAILVNLDPFY
                     ISADYVFGSGFPSAPAILAEQADDLTYSRLDASFIYKFLDRRLKGEVGLSVLNVMNTE
                     NIKYANFERVSTNADNSVNLYSEAIPITPALYLKLAM"
     gene            complement(13335..14201)
                     /locus_tag="PKZ74_03805"
     CDS             complement(13335..14201)
                     /locus_tag="PKZ74_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPM91912.1"
                     /translation="MKQFYDHIDPINSEKPDRGDIFRRLEVPVSRTREEVWEYLEKRI
                     DEPSSPKLLRFRPRWIIAAAASLILLAGVASVLFYTSRTIVSAPGQHLSHILPDGSRV
                     ELNADSKISYKPYRWMFSRIIELSGEGFFEVEPGRTFSVVSTLGTTTVLGTTFNIYAR
                     DSEYKVNCLTGKVKVVSTTESFVVLSPSQSAVIENDGKILVSRQSNEANPVGWLDNMF
                     DFKAVPFTRVMEEIGRQYGVSIQADIPEGIIYTGHFSKNRPLEETLGVVCKPFGFTFA
                     RIAENEYIISQN"
     gene            complement(14205..14684)
                     /locus_tag="PKZ74_03810"
     CDS             complement(14205..14684)
                     /locus_tag="PKZ74_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM91913.1"
                     /translation="MTRSQFKELFDNHFDPVRNYIYYRSGDSETATDIAQEAFLTIWE
                     KQPADLTHITSLLYKIAGDRFISLYRREKVMEKFRLSVKPDQTGGSPEEQVIFEEMKS
                     RYEKALALLPEKQRAVFLMSRMDRMTYHEIAERLGLSVKAVEKRMNLALAFLKKSIE"
     gene            complement(14708..>14975)
                     /locus_tag="PKZ74_03815"
     CDS             complement(14708..>14975)
                     /locus_tag="PKZ74_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91914.1"
                     /translation="QGSDTGNPWDDIYLLTGTASGTRPSGLTWEQEITSPLRIELACR
                     FIVSGTIEIRPQDRPVWVLDYGTGECDNVATVTVNGQTYTIYLD"
BASE COUNT         3875 a         3676 c         3507 g         3917 t
ORIGIN      
        1 tatcacctat actaccaatg gctctaaccc acagacagtt ggcgccaact cctccccgta
       61 tattttgccc gtgtatcctg ctgtgcctac tacttatgaa tttaccgcgg tcgaagatat
      121 ttactgttcc ggcttggcct ccggagtggt tgaagccgat gtcgatcctt caccgacagc
      181 aaacgcaggc aatgataaaa ccatccccta cggaacctcc accacgctga acggacaagc
      241 ttccggcgga tcgggaaatt accagtattc ctggcaaccg gccgctaaac tgaccaaccc
      301 gacggcacag aatccgctga ccgtcaatct gaccgaatcg actctgttta cgctaaccgc
      361 gaccgataat gtcggtggat gctatgatct ggacgacatc atggtgagcc tgtcgggcgg
      421 accgctggga tgtatccccg ttgccgaccc gcccgcggtc tgtaccggcg atccgtcgca
      481 tcttttgccc tatgtttcag gtggttcagg aacctatacc tatctttgga gctccaatcc
      541 tcccgggttc tcatcgtacc tgcccgaccc ggtcgtaacc cctgcacaga cgacaacata
      601 cagtgtacag attaacgacg gctataacgt catttccggc aatgttaccg ttacagtgca
      661 cccttcaccc atccccgatg caggagaaga tgtcacaata ccccatggaa caaatacaat
      721 tcttcacgga tcagccggga atggaagcgg aaactatgct taccactggg aaccggctga
      781 cctgcttgtc gatccgaatg tcgcttctcc acagacagaa aatatttacg gaacaacgac
      841 tttttcgctt tttgtgactg atctgacaac cagctgcgaa tcgcagtcgc ccgatgtaat
      901 gaccctgatc atatcgggag ggccgctggc cgtggtgcct gcagcttatc ccgaaacggt
      961 ctgcctgggc gaaacagtta aactctatgc caatgccggc ggaggggcag gtgcagaaaa
     1021 ctaccagtat agctggctgg gttccgacgg atttaattct accctccaga atccggaagt
     1081 aacacccaac caggtgggta ctatatatta tacttgtact ctcgacgata gctttaatac
     1141 tacttttgga agtgttgccg tcaatgtact cactacgccc gagatcgatt ttggatttac
     1201 cagtaaaacc gtctgcgttt tcgatacagt tatcctggac ccggatgttc cgaatgcatc
     1261 ctatttatgg tcgaatggag ctacaacaca gacaatcaga gcggcctcaa ccggaattgg
     1321 tttcgacatg caaacctata cggtcacggt taccagcctg agcggaggct gccagtctga
     1381 gcagtctgtc aatgtgattt ttgatttcac agcctgtaca ggcgtcgggg aggatttatc
     1441 ctctgccatt tgccagatat acccgaatcc cggggatgga aatatcagaa tgatctttga
     1501 tccggagttg aaacaggcag aaattgatgt cctgagtata cagggatacc ctgtctcgga
     1561 tgtctttact tattataagt ccttgatgaa caatgaactg atactgccgg tcgggtattt
     1621 gagcgaaggg atgtatttca tcaggatctc agataaaaag ggtattaacc aactttataa
     1681 atacatttta aggaaataat caggttcgat gttttgacgg aagagtcgtt atcaggattt
     1741 tggtagtttt attaaaatgt tatctttggc ctgaaaattt gaccggaaaa aataaactct
     1801 caaacccagc atgaataaac tcgtttataa aaacttgtgc ttctcgttgc cgatgctgtt
     1861 tgctgcggct atattgctga taatcaacca aagcgcaaat tcacagggaa ttcttaaagc
     1921 cgaactgcga tcttccaact gcccggtagc cgaccacgaa gccgatcgat ggtattttgg
     1981 ccagaatgca gcgctcgact tcagaaacca ggatccgttt gccgaccaga ccaatgccat
     2041 gcttaatgtt cctacatgcc cggccgtggt gtccgatagc aatggtacta ttttgatcta
     2101 taccgacggc ctcaatgtct acaaccgtta caactcgctg gttgcaaacg gcagtgggtt
     2161 gcatggattt gtcaacggag cacaaccggt gctcatcgtt cctcatccgg gaaatgacag
     2221 tatctatttt atattcactg caggccgacc gaaaatgaac cccctggatc ctaccatgat
     2281 ctacggattg gaatacagtg ctgtcaatgt ggtgagaaat ggctttagcg gcgaagtgac
     2341 caaaaagaat catgtactgc tcggtcccga ggtctcaaca aaacttaccg ccgtcagaca
     2401 ttcaaatggc gtcgactatt gggtcgttgc ccataaattc aactccgatg agttttgttc
     2461 ttttttggtt tcctcaaatg gcgtcgacac taccgggtac agatcgagtt ttgtcggaac
     2521 gacccactct gcgccgggtg aatccaatac gtttatcggt tttatgaaga tcagtccgga
     2581 tggaactaaa ctggctactg caattcatgg ggccaaaaga ttcgaaatat ttgatttcaa
     2641 cgcttcaaac ggagcagtat cgaatgagat cgtttcagat cctatttttg atgatgccta
     2701 tagcgtcgag ttttctcccg attcgcgcta cctttatgga actgctactc ccgttaatat
     2761 cgagccgctc gatccgcctc actcctatat ttaccagttt gatgtgaacg atggaaacca
     2821 gattttcgac ccatcattct atacagtaat tgctcaggat acactgggaa gttattttgg
     2881 aggtatccag ttaggtaccg acgggcgtat ttacgtttcc cgctcgccca atggttattt
     2941 ctccctgagc gtgatccaaa atccgaaaag ggatgggctg gcctgcaatt tcatgacgaa
     3001 tgcactgcct ttggccggta agcgaagcta ttacggcttc cccaatttca tgcaatccta
     3061 cttcgatttg ccccatttcg atgtcgaaaa tgtttgcttt accgatacga tggtctttac
     3121 actgcaaaat aattctaaca taacaaacgc tacatggcag ttcgatgacg gcggcccggt
     3181 cacactcaac gcagttcaac ctacatatac tttttccgga ccgggaacct atgacgtcag
     3241 ggttaccgaa ttcaacggga ccgattccta tggcccctac acggaaacag tcgtcgtccg
     3301 tgaattgccg accctggata tcggtgacac cgtttacatg taccccgggt ctccgattat
     3361 actggatgca ggcgaaggct atgaaactta tgaatggtcg accggagaat cttctcctaa
     3421 aataaagatc tatgaggcgg gaacctatac cgttaccgtt cagaatgaat tgtgctgctt
     3481 taaatcggat actgccgaag tgatctactt cgatgtgcag gtgcctaacg catttcgtcc
     3541 gggtggagca aaccccgttt ttaaagcctt cgcttcctcc ccggatgcta ttgtcaattt
     3601 caaattgttt atttacaacc ggtggggcca gcttttatat gaaggaaatt ctcttgatca
     3661 gggatgggat ggtaaagtga aggggattga tgctcccggc gatgtttatg tgtggcttgt
     3721 aaattatgat gtggaaagat ccggcaaaat ggtcactata gcttataaag gaaatgtaat
     3781 ccttttgaga tgagatggat aagaagtatt atatccggat agccgaagaa ctcaatatct
     3841 ccaccgaaca ggttatcaat acagccgccc tcctcgacag cggcgctacc gtccccttta
     3901 ttgcccgcta ccgtaaagag ctcacaggaa gcctggatga agtcgtgatc accgatgtcc
     3961 gcgaccgcct gaaacaactg ctcgacctgg ataaacggcg agatgccatc ctcaaaagca
     4021 ttgaagaaca gggtaagctt accgatgaac tcaggggacg catcctggaa gtgatgacga
     4081 tggcggaact ggaggacctc tatcttccgt ataaaccgaa aaaaagaacc cgtgcgacca
     4141 tcgccaggga aaaagggctc gagcccctgg ctaagatcat catgtcgcgc tttgaacaag
     4201 atccgtaccg ccgtgcaaag cagtatgtca atgcgcagaa aggagtagcg gatgaagaag
     4261 aggcactggc gggcgcccgc gatatcatcg ccgaatggat cagtgaaagc cagcccgccc
     4321 gggcccgggt gaggaaattg ttcctcagag acggacaaat ctattccagg attgtcaaag
     4381 ggaaagaaat agaaggtgtt aactatcgta attactacga atggaacgag ccggtatcaa
     4441 aaatcccttc ccataggttc cttgccatga tgcggggaga agccgaggga ttcctgaaaa
     4501 cagatgtgta cccccccaaa gaaaaagcga tcgaaacact cgaggatatt ttcataagaa
     4561 acgcgactga tggttgtgcg gaacaaatcc gtaaggcggt aaatgatagt tatggccggt
     4621 tgctccaacc ttcgatggaa aacgaaatta aagccgccgc caaagagaaa gcggataaat
     4681 atgccattac cgtttttgct gataacctga aacaattgct tatgcaacct ccgctcgggc
     4741 aaaaaaatgt tttggccgtc gatccgggat tcaggacagg gtgcaaactc gtttgcctcg
     4801 ataagcaggg caaccttctt cataatgaaa cgatttaccc ccatccgccc gagaaccagg
     4861 tgaaaatggc ggagaaaaaa atcctgtcgc tggtggatgc atataaaatc gaagcgattg
     4921 ctatcggaaa tggtactgcc ggtcgtgaga ccgaacgatt tatccggaat atccgttttg
     4981 ataaagacct gatagccgtc atggtgaacg aaagcggcgc ttcgatctac tcggcgtcga
     5041 aaattgccag ggatgagttc cccgattatg acgtcaccgt caggggtgcg gtctcgatcg
     5101 gccgtcgcct gatggatccg ctggccgaac tggtaaagat cgacccgaaa tcgatcgggg
     5161 tagggcagta tcagcacgac gtcaaccagt ccgatctcca gcaaagcctt gaggatacgg
     5221 taacgatctg cgtaaaccag gtcggggtcg aaatcaatac cgccagcaaa gaactattgt
     5281 catatgtttc cggcgtcggc ccttcactgg ctcagagcat tgtcaatttc agaaaagaga
     5341 atggcccgtt caactctcgt gaccagttcc ggaaagtccc ccgcttcggc gataaagcgt
     5401 tcgagcaggc tgccggattc ctccggatcc gcgatgcggt gaacccgcta gatcgaagcg
     5461 ccgtccatcc ggagagctac catgtggttg ccgaaatggc gaaacgtacc ggtgcttccg
     5521 ttgccgatct gatccaaaat gatgatattc ggaaaaagat caatctcaac gattatgtca
     5581 gtgaaaagat tggccttccg accctgacgg atatcctgca ggaactggcc aaaccgggac
     5641 gtgatccgag gcaaaaattc gacatgttcg aattcgccaa agggatcaac agtatcgacg
     5701 acctggtgcc gggtatggta ctccctggaa tcatcaccaa tataacggct ttcggggcct
     5761 tcgtggatat aggagtccac caggacggcc tggtacatgt cagccagatg gcaaaccggt
     5821 ttgtacgcga cccgaaagag gtggtgaaaa taaatcagaa agttatggtt aaggtgctgg
     5881 aggtggataa ggagagaaaa cgaatcaacc tcaccatgaa ggagatttga tgatattttg
     5941 attttttgat attgtgaaat tatgatattg tgaaattatg atattgtgaa attatgatat
     6001 tgtgaaattg tgagcttgtg aagttgtgtt cttatgaaat cttgatcttc tgatattatg
     6061 aaaagcgtag gtattatttt ggttttttgg attcttgcgg gtctggtatt tacgggatgc
     6121 gttagggagg aggagtcaat tgagttaaaa attgcccttt cgaaaggcag cccggagaat
     6181 tcctatgcca attattacaa ctgggttaag tcgcttgatt ccaatgctgt gagtcttgat
     6241 atgtacgata tgccccttga ttctgccctg gcgcttttta aaggatgcga tggattgatc
     6301 attaccgggg gaaccgatat taatccggcc tggtatgacc aggaagagga ctcgctccgg
     6361 tgcatgacca tcgaccacta ccgcgatacg ctcgaaatga ggctgatcga ttcggccctg
     6421 gcctggaaaa tgcccatcct gggcatctgc cgggggcatc agatgctcaa tgtctatttc
     6481 ggcggcagcc tgatcatcga cataccctcc gattatgata ccctggtcag ccaccgatgc
     6541 cccgatttta atacctgctt ccatccggtt acccttgccg acggctcact gatagcggaa
     6601 cttgcaggcc agacggccgg aacagtgaac agcaatcacc accagggaat aaaaaacctg
     6661 gcgcctgcgt tgaaaatcgt ggctttcact gaagataacc tgccggaagc cgtggaatgg
     6721 aatgaacctg ccggcaaaac cttcctgatg ggcgtgcagt ggcaccctga aagattgggg
     6781 aacgataatc ctctctcgaa agaaatagga atgagatttc tgcgggaatg caggaaataa
     6841 ccctggtcac taatccctga ataatttatt tatcctgtaa ttctttataa cggaagcacc
     6901 cggtaatatg gtcgttgacc atgcccgccg cctgcatgaa agcgtaacat attgtcgagc
     6961 cgacaaattt gaatccccta tccgccaggt ctttgctcat cctgtccgat acttctgttt
     7021 ttgcaggtat atcctgcatc gacttccagt tgttccggat agttgtgtgg ccgacaaaag
     7081 accagatata tcggtcaaaa gtcccaaact ctttctgaac tgccaggaaa gcattcgcat
     7141 tggatatcgt ggcccggatc tttgacctgt ttctgattat cccttcatct tttaacaatt
     7201 cctcatattt ggcttcatca tacctggcta tcttctccgg gataaagtta tcaaaggccc
     7261 gcctgaaatt ctccctcttg tttaaaatgg tcttccagct taaacctgcc tgaaatgcgt
     7321 ccagtaccat gaactcgaat atcttgcgat cgtcatgtaa tggaactccc cactcctcat
     7381 catgatactc ttgcatcagc ggatcactga tccagccgca cctgttttta gtcaactcct
     7441 ctctcatccg atcattgggt taattcttcg ggttgagtat tcttattgta cttctcattg
     7501 aaaacataaa tcagccgaac cgtaaacaag tgattgtact gcttgcgttc gtatggatcc
     7561 ctgatgccat agatgacatc tttataaatt cgctccctga tggggaaaat ggagtaattg
     7621 aaccggatat tgccttttaa ttgtttccag atcctgaact gcaggtcgaa tatgacattc
     7681 cagtcgttat acctgaattt gaccgtatcc gaatagggtg caatgttgcc cccatgttcg
     7741 atttcttttg aattgaccag ccttccgtag gaaaaaccca tacccacagt gtatctttct
     7801 ttgtcggtat aatgggcagt gaggggtacc tcgacgtaat taagttttag gttatattcg
     7861 cccgtataag tttgagtttt aactgggact accgtactgt cggtaatggt gtgaatatat
     7921 tgcgcttttt cccgggcccc tttctggctg aagacggttt cgagggtaac atcccacttc
     7981 ttacggatcg gtagagatac acccagcgat aaatgccccc ccgctttgtt gtaaccgacc
     8041 acttcatcgc catccacctg cgacaggttc attccggctc ctgcaaaacc caggaaccgc
     8101 tgtgcatcag ccgaagaaac cgatgccatt agcagaccaa tgatgaataa atatcggagt
     8161 aacttcattt tccctggatt tcttctacaa cggaatattc aggcagttta ttttttcgat
     8221 acatcatttg tttaccggat gcttttgttt gtgtaaaaat caacaggggc ccaatcatcc
     8281 gtcagtaccg gtacacctgc ggcaatcagg tgtgtcacat tatttttcag catagccgac
     8341 aattcgggat cggagctggt cagccctgac ttcgatgagg tttttaaggc aaccagaagt
     8401 gtgccctgca atacatcttc atccaccggg ttatcggcag caaagatata aacatgcggg
     8461 aaaacatcgc ggtaagtagc tgtttctgcc tgcaggaaaa gactcgactc tccgccgatg
     8521 gatgagatga tattcagaat cacacacccg ccttcattca gcatttcgta agtcttttgc
     8581 gccgcttccc gggtggtcag gtgccagggt aatgtataca gcgatttata tgtgtcgcca
     8641 aaaatgacat cgtaacggta ttccgaccgg ttcaggaagg tcctggcatc ttcatggaaa
     8701 atccgcatgc gcggatcatc ccgcaggttg aaatgctgcc gggccagttc cgtcagctcg
     8761 ggatcgatct ccactacatc aagagtggcc tgcggatatt gctgaaggaa atatttcggg
     8821 taactgaatg ccgctccgcc taatatgagc gctgacctga aaccgggggt gaaatgttcg
     8881 gccagccggt aaaaccgcat gtaagggaaa accggttccc ggctgtcgtg aaactctgcc
     8941 gacgaactct cgttgttgat ccgcataaac cggatgggcc tgccggtggc ctggtccgtg
     9001 gcatcgtaaa tccagacacg gctgtattgg gtatccacat caacataagc ccgttgaccc
     9061 gattgatccc ggtagagaat gtagctgttg accagtgtca gcatgactgc cgctataatc
     9121 cccgccggaa tttttgagat gatgaaaaga aggatggata tgagtaaaag caggatcgtg
     9181 attgcaatca gaatgcttgt cgttcctata gccgggatca ggtaaaatcc cgccgcaaag
     9241 gtcccggcaa tgctgccaac cgttgaaatg gcataaagtc gtccgacagt agcgcctgaa
     9301 gtctgcaatg ccttgatctt cagccttaca gcgtatgggg ataccattcc caggaatacg
     9361 ctggcagggg caaacagaaa tacggacgca actaccgatt gccatttcaa accgttaatt
     9421 acttcaggaa gaaacttcag aaagggaccc ttgacaaggg tcgtgaagcc tatgaacaga
     9481 ccggcggcca ggatgatcca ggccagcgcc ccgggagagg gtttccggtc ggccagccga
     9541 ccacccagcc agtaacccag gctcaggctt cccaggatga tcccgatcag gctggtccag
     9601 atgaagatcg aagtgcccag gtaaggcccc agcactctcg atccggcaag ctcaaagatc
     9661 atgacaaccg caccgcacaa aaaaacgatg atttccagta gatgtttttt caagtcgcgt
     9721 atgatttcaa cggtaaagat aatccgattt tgcaatctga tgcttcaacg ggtcaatccg
     9781 tcggcgctgg aggggttttc ttccggcggg taagtccgtc ggcgctggag ggattttctt
     9841 ccgacgcgta aattcgtcgg ttattaggct agaggttttc gccgcaggcg ataaaattct
     9901 gttgccggat caataacaaa aatcaataat tccccgtagg gattttcgcc gtaggcgata
     9961 aaattttgtt gcaggatcaa ttacaataag caataattcc ctgtagggga taaacaaaac
    10021 cttaaaaatc gagataaaat tccccggtga gatttttgta ttcctttgaa taagagttat
    10081 tttcgttttt aatatgaagg gtttgaataa ccggagccac cacgctgcat ttatcaaacc
    10141 ggatgataac cagttgaata ttcctgttgg gttttgggat gatataaagc aacctgcttg
    10201 gaaataaatt gttttgtccg gccttctgga tgaactcctc acattttgct tccggtaaaa
    10261 taacccaaaa gctgccattc ggatttaaaa tttggctggc aatacgaata agatcatcat
    10321 aaccgagcga atggtcatgc cgcgaaagat tccttctctg tgaaggagac tttaatgaat
    10381 tcgaaaaata ggggggattg gaaacgataa ggtcatattt cttgctacaa acatcggtat
    10441 aatcctggag cgaaacatta ataacattta tcctctcttt ccacgggctc ttcactacat
    10501 tctcctttgc ttgcttaacc gattcttcat cgatatcgat agcttcgatc aaagcatcgg
    10561 atcgctgcgc cagtatcagc gcaataacgc cgcaacccgt tccgatatcc agtatcgaag
    10621 cggctccggc cacatcaacg gcggcaccca gcaatacagc atccgtaccc accttcatgg
    10681 tgctccggtc gtccttcacc ccgaaatgct tgaagtaaaa ggtgccggtc gctttcccgc
    10741 tcgccttcaa ctttccgcta ttataaatat ttgtcatatt accagctgtc caatccctcg
    10801 cttcgcccgg ataaggctgt cctctacatt gccagcttca aatacaatgc cggcgtaatc
    10861 ggaatagcct ctgaatacag gttgaccgaa ttgtcagcat tcgtcgagac ccgctcgaaa
    10921 ttggcgtact tgatattctc cgtattcatc acatttaata ccgatagtcc gacctcgccc
    10981 tttaaccgcc tgtcaaggaa tttatagata aacgatgcgt ccagccggct gtaagtcagg
    11041 tcatcagcct gttcggcgag gatagcggga gctgagggaa aacccgatcc gaaaacataa
    11101 tcggcagaaa tataaaaagg atccaggttt accaggatcg cggctttcac ttcatgccgc
    11161 tggtcctgcg gcgcacggcg gtattcatcc tttttgaaat attcgaataa ctcctccgtt
    11221 ttgccaacag tgtacgaaat ccatgctgaa tgtcctttgt aatccttctt gatcatcagg
    11281 tccactccgt acgaacggcc aattccttcg taaatccctt cgatgttcag cgccgtattc
    11341 cggatatagc gcgtcagtcc tgatacattg cggtaatatc cttcaatgct gacagtcaaa
    11401 tcattatggt tgtaggaagt cccgagaaca taatgataag ccttaaggac cggtatgctc
    11461 tcgttgtcgc aaaccgtcca gatgtaccgg taattgccca tatcatccaa aacggaactc
    11521 tttgttaccc actggtcata aagtccccag gcaccgttaa ttttaaagga ttcacccagc
    11581 ctgaccgatg ccgataacct gggttcaaag taaaatttct tcagattatt cgcacgggtt
    11641 aaacggaaac cggcttttat atcaataata ttcaatagct tgatattgtc ctgaaagtat
    11701 agggtcatcc tttttgcctc ttcgtcgaac cgaactcgcg gtatgtcaaa agtgtcttcc
    11761 gccagttcga tccggttggc catataaatc attcctcctt ccaggatatg tgactcatga
    11821 accggaaacc ggttatcaac ccgcaacgaa ttctcccgga tcgagttaaa cgcatacaag
    11881 tcaatcctct gctccgtttt ttgctggatc accttttcga tctccaggtt atccgtaaat
    11941 gtcgaattta aaacggtata attcaggctg aaatgggtcg aatatccatt tttccaggat
    12001 tttccgtaaa ataccgatcc ccccgattgc ctgttttttt cagaagtgtt ttttttgatg
    12061 atgatatggt tgaaaggttc attgatggaa tacgaaaaac ggtcattccc gctataaaga
    12121 ctgatataaa aaagatccgt gtttctgatg cgggccgaat atttcaggtt catatccccg
    12181 aaacggtaat ctggaataat gttgacatcc acattttcca ccgcattatt tcccctccct
    12241 cttgaaaaaa ggtcattgac gtcccccggg ttatacaact cataatacgt atgacgaagg
    12301 gcgaatatca gtgaccctgt tttaaacaag ggtatttcaa ccatgccgtt catagtcatg
    12361 ttgttgatgt cgacggtgaa tgaggtcttt tcggtatttc cgtttttacc ggtgatattg
    12421 acaatgcccc cgacgcgttc cccgtaccgg gcatcaaaac ctcctttgta aacctcaata
    12481 tctttggcca tcaggggatt gaaggtgctg atattgtcgt tgaagttctt taacccgtaa
    12541 agtgtgaacc cgtcgaagat cactttgctc tgcccttcat aacatcccca gacaatcagt
    12601 tcgttggttt gttctcctga ggcgaggatg ccgggctgaa gccggagcag gttaaaaacg
    12661 gagttgtcgc cgaaccctgg aaggaaattg gccacctggt ggttcagctt cattactccg
    12721 ggctcacggc cgatcaggga tgccctttct acgaccgaat gggtgataat gacctcattt
    12781 aaaccgataa agttgggtga taaactgaac tgcacattcc ggttccccat aaccagcgtg
    12841 tcgagaataa agtaaccgag atgggtcgct ttgatctgaa aaacactgtc ggttgaggaa
    12901 atataactga agctgccttt gatatctgtc gctatgccgg tgttgttgat gatgacgtgc
    12961 gagtagggca ggggctcccg cgtccctttc tcgatgacct gccctgtgat actgtaggtc
    13021 ctttttttcg gagatttttt ttgggggatg atgatgaata catcaccctg tttttcaagt
    13081 tcaaaaggca ggttctcaat taccttccgt atcgcttctt ccggagaaga gaattcaccg
    13141 gtgatattaa ccttgtattg agataaactc ttttcattga acgatatctg aagaccatac
    13201 cggcctgaaa aatcatataa tacttcgctg gcgggcttcc cctgtacctc caggctgagc
    13261 ttctggcctg aggccaatac cggtatgacc aggcagatga ggaaggcaaa tgctttggcg
    13321 ggacttgtca tccgtcaatt ctgagaaatt atatactcgt tttcagcaat cctcgcaaaa
    13381 gtaaatccaa atggtttaca aaccaccccg agcgtctcct ccagcggcct gtttttggaa
    13441 aaatgtcctg tgtaaatgat cccctccggt atatcggctt gtatcgatac tccgtattgt
    13501 ctcccaattt cttccattac ccgggtaaac ggtacggctt taaaatcgaa catattatcc
    13561 agccaaccga ccggattggc ctcattggat tggcgcgaaa ccaggatctt tccatcattt
    13621 tcaataaccg ccgattggct gggactgaga acgacaaagg attcagtggt cgaaacgact
    13681 ttcacttttc cggtcaggca attgactttg tattcactgt ccctcgcata tatattaaag
    13741 gtagtgccca gaaccgtcgt agtccctagc gtagaaacaa cagagaaggt acgcccgggt
    13801 tcaacttcga agaatccttc gccggaaagc tctataatcc tggaaaacat ccaccggtaa
    13861 ggcttgtaac tgatcttact gtcagcattt aattcaacgc gtgatccgtc ggggagaata
    13921 tgcgaaagat gctgtcccgg agcgctgaca atagttcgcg aggtatagaa aagcacggaa
    13981 gcaactcctg ccagcaggat aagagatgcg gcggcggcga tgatccaacg gggcctgaac
    14041 cggaggagct tcggggatga cggctcgtca atcctcttct ccaggtactc ccatacctct
    14101 tcccgggtcc tggataccgg aacctccagg cgccggaaga tatccccgcg atcaggcttt
    14161 tcgctgttta tcgggtcaat atgatcgtaa aattgcttca ttcattattc tattgatttt
    14221 ttcagaaaag cgagggccag gttcatcctt ttttcgacag cttttacact taaaccaagc
    14281 cgttccgcaa tttcatggta tgtcatccgg tccatccggc tcatcaggaa aacggcccgt
    14341 tgcttttccg gaaggagagc aagggccttc tcatatcttg atttcatctc ttcaaagatc
    14401 acctgttctt ccggcgaacc gcccgtttga tcaggtttca cactcagcct gaacttctcc
    14461 attacttttt ctctccggta caggctgata aagcggtcac cggcaatttt gtacaacagg
    14521 gatgtaatgt gtgtcaggtc tgccggttgt ttctcccaaa tggtaaggaa cgcttcctgg
    14581 gcaatatcgg tcgcagtctc agagtcaccc gaccggtagt agatgtagtt cctcaccggg
    14641 tcaaagtgat tgtcaaagag ctctttaaac tgcgaccgtg tcaaagttgc gcgcttctgt
    14701 atgtgtttta atccaggtaa attgtgtagg tctggccgtt gacggtcact gtagcaacat
    14761 tgtcgcattc gcctgtgccg taatccaata cccaaaccgg tctgtcctgc ggacggatct
    14821 caatggtgcc cgacacgatg aaccggcagg ccagctcgat gcgaagcgga ctggtgatct
    14881 cctgctccca ggttaggccg ctgggtcttg tgccgctggc ggtacccgtc agcaggtaaa
    14941 tatcatccca tggatttccc gtatccgacc cttga
//
LOCUS       DAOPNH010000045        14965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4003_length_14965_cov_7.357009, whole genome shotgun sequence.
ACCESSION   DAOPNH010000045 DAOPNH010000000
VERSION     DAOPNH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_14965_cov_7.357009"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKZ74_03820"
     CDS             complement(<1..283)
                     /locus_tag="PKZ74_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91915.1"
                     /translation="MKTMPILKFFGVIVLSLLVSTLTAQVKIGEKPDQMDPSAILELD
                     SANLGLLITRVSLADVYDIWTIPNPANSLLVYNTNFGGGSNPVSPGFYYF"
     gene            complement(472..1773)
                     /locus_tag="PKZ74_03825"
     CDS             complement(472..1773)
                     /locus_tag="PKZ74_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91916.1"
                     /translation="MKPKSQKFASRFTLHASLLLCSLLTVHCSLNAQQYSWKNISANL
                     PDLPYDTVIFGGDTMVSMIRASHFLDDSNGWIVIHNAVDSSFILHTANGGESWEHRST
                     HKTAFNAIHMRDQLTGFAGGQDGGIIYKTTDGGYTWNFHWTLGTTLAEIEFPPLPADT
                     GYACGMNGSLMMITPTGGNIVDVNVNSDLHGLSFPVNNAQGWLSGWNIVRHYVDGTWY
                     ADQIHITGYHNDIEFIDNQTGWTCGDRIMHTEDGYNWVNQVEGDQLDGDLFGISFANS
                     ETGCAAGTVGQVYFTANGGGTWEKLDLGTTENLIDVHMTSPTCGYITGQGKQIFKYTQ
                     INGEEETGRPGDMEREEVEVWPNPTTGHFKVQSSKFKVEFQSLELTDICGLIVESNNN
                     ITMEQLNNAAIDFDISYLPAGIYFLRISLENQTIVKKIIKL"
     gene            complement(1877..3220)
                     /locus_tag="PKZ74_03830"
     CDS             complement(1877..3220)
                     /locus_tag="PKZ74_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM91917.1"
                     /translation="MKPKTQKFASRLTLHCSLILCSLLTVHCSLNAQQYGWVDISGNL
                     PDFYNDTVIINDGADTLIANISGISFLDDNHGWISTYHPFDGEPSAVLETTDGGQNWT
                     EHSAPYTGADIHMIDELNGYFGAINGFIYKTTNGAQSWTLHGLLAAPLYDLGFPPRPA
                     QNGFAGGKDGHLAQISPEGVFPVDLGLAGSVYCIDFPSEERGYALLDYQMIIYYLDEE
                     WHVEASYPFSSKDWLYFRNDTVGWCVGEMFLKTIVGIDWYRTDPEFVQTGAMMGVYFT
                     DLNNGWAVGTQGQIAYSNDGGEDWTMLEHHLTDEILTGVLFTSPTNGYIIGGEKTLLK
                     YTELNGEPETGGQGDREKGSVEVFPNPTRGKFQIISTKHQTNSKFKIPNSKLQVEVVD
                     LFGKPVAISPLHRMGEGPGVGAGTLEFDLTGCPAGIYFLCIHLENQTIVRKIIKL"
     gene            complement(3321..4880)
                     /locus_tag="PKZ74_03835"
     CDS             complement(3321..4880)
                     /locus_tag="PKZ74_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91918.1"
                     /translation="MKNITFLPLIILIHLFSMGYGQPPGRETLAPSSPDSVITYKFDA
                     QGDSTLNAKMTYRYDNHLRVIESEKFVREQSLNLWIPNRKEVREYDNHDRRTLWAIYE
                     WDNNEMAYKGWMKETTMYDNNGNQAEYHYFTWDYTSYDWGNRFWDLFDYDNENKLTRR
                     DHLEWNTVSEYWDTASYEIYEYSGSGFLEYLTTYEPDEGSGIIYPSDRGDYEYDEFGN
                     VFKITHQIYNPNTEQWYWSIRFQYNYDEQNRKNHSVYSLYDEQGEMWVEEEKEDWGWD
                     DNDSLTIYAYYRIGEDLVTWYPNLKTEMTYNNRGRLIHYRGYSGNDQAQWVPTYERRY
                     PYQNDTLLMADSLFQWIEDDQVWEFVDCHNYRYDSLFRMHTDSYYKLVVNTGEYLLSF
                     RDYYFYSESSGIEEHEEGEKVKRESVEVYPNPTHGKFQISSTKFQTNPKFQAPSPKHQ
                     TNSKNQIQKIEVVDLSGKEVRISPLHRMGEGSGVGAGTLEFDLTGCPAGIYFLRINLE
                     NQTIVKKIIKL"
     gene            complement(4883..7663)
                     /locus_tag="PKZ74_03840"
     CDS             complement(4883..7663)
                     /locus_tag="PKZ74_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91919.1"
                     /translation="MKPKSQKFASRFTLHASLLFLALCPVLLATGQNHKWYYNYNTGK
                     SEEGKDIVCGSDGYIYIAGTEYDEADYDIIVIKLDKSGNQQWVYSYEGQPDKTMVVAE
                     IQAGSDGNIYVCGTANNAEDNNKFLVISINPEGGLRWEYIYDVDGNYPSEAFSVAYGG
                     NGMVYAAGKANYDFLVAGINAGSGEQEWIYWFDGGCPYALCDDQASAITVGNDGNIYA
                     AGYTHEATEPQLTILSLTPEGSKNWKYLFPFSEGRTWATDIVYGDDGRIYASGLIYSN
                     IAVICVDATGQYQWNCNVDGPGPEPYWGETCYELIYGFDNNIYVTGRAGGRDNQVDTD
                     MDVAVIKVDLQGSPVWFYRYEGLYGDHDMAFSITQTPDTNVHVAGYFCGLLAEAGTIS
                     LDHRTGRDLWVMRYVGPAIDMDVAYAITSDDDGYLYVTGYDFKANRLHDVYAWKLQPP
                     KNTDGFYNLEGYATWGEGHSVLETPDKGFLIAGYQGSSMTSSTYNMRLIKTDINGDTL
                     WTRNYGGNNEERAFDVAICPDNGYILTGFTKSFGAGGKDLYIVRTDENGNMQWEKTYG
                     IETDEEGYSIVTATDGGYFIAGKTYQYDGAGDLWFMKINAQGDSLWTKRYGGDRRDEV
                     GEVHRTADGGYIFAGTRGHALTLGYITDIYAIRFDAAGDTIWTREIGSEDYWDAGGDI
                     LEQDDGTFLLVGYYLNKEYIAKLDADGKTLWEKTSETEQNGGFTTISKKPDGNILLSR
                     NGFGSQYLMNVRTYDTEGNFISSDTMGWSPGYVLYPTTALANDAQPTSGGGYVAVGKG
                     NIAGNANNWNIVLYRKGGTLTMLPLPPLGIKELPFINTNHKTQPAIVSPNPVNDQAII
                     GFTLSSQEHAVVEITDIAGRIVYKSEPRYFPEGDGQFELNNMHMRNGLYNCRIIAGNE
                     IFTGKFIILKNY"
     gene            complement(7674..8798)
                     /locus_tag="PKZ74_03845"
     CDS             complement(7674..8798)
                     /locus_tag="PKZ74_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM91920.1"
                     /translation="MTPLTEDIRNEKLIFRNWENALPVKKNFSGGILVTGANSFLGCH
                     VIKALQQNFQGDIHLLVRAPNDFEAVARMQQSFDRWELGQFNIRRFNIHRGDVCLSGM
                     GMNPEEYRRMCRKTRQVIHLAMTPMYHLPYHHFQQTWIPELERMISFCMDKEYPKFLH
                     YTSSYNANFFVTDEDFQSLNRNAWQSGYAGFKWVAQEAIRNASRQGLGSCIYDVPLVL
                     GSERKGICPSHYSIWIILDIFLKTGYFFPFSFRIIPVDMLAEMIVLNADAAGIGRASG
                     FIRPFLEEPVTDSLFARTAANLLGLKEATLETVRQNCINKLRFDFMMPVNFYSLLEKV
                     NGLPPVFPEWYDRGVLPVTPMVFMSNLNRIMSVKKEMIKT"
     gene            complement(9101..11107)
                     /locus_tag="PKZ74_03850"
     CDS             complement(9101..11107)
                     /locus_tag="PKZ74_03850"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM91921.1"
                     /translation="MKSFSKWAILLVFAVVSGFSATITEQDTARVNHLLRKARSIHYS
                     NPDSAVFFYENILSHTKVDTNLIHPGISDLEKAYLVSVIRAQNYIGNIYYYNDEYNRA
                     DYYYRRSLTLARKAGFSDFTANSLYDVGYTFYVTNSYKKAAEYFKESYEMFYESDNNQ
                     GMFDAIQAWALAERHLGNLEQSDSCYRRASALAAAIGDSTQIADVQLNNGILLCELGN
                     LEEGTRLFQTALDYYDKKNNREAVSLALLNIGVVMKMLGEFEKAEDYILRSAAISEAL
                     QQKSQLVIRYSNLADLYMEMKRNETAFEYCNKTLSVASEIGAQPFITECNLLLGKFYY
                     LEGNYDKAREFLAAAAESVKKTNDKPMMINVLLWSSRNLLEHGKYSEAIRTAREAFDM
                     AEELMIHAGSKDVAMVLSQAYEKNGQPGEALFWQKQYQKLSDSLNFANQHKEITRIEA
                     KYNYDKKEKENEILRNKATLQEQKLRSRTIVGIAFVTGLIFSIIIIILLVRRSRDARL
                     LFQQQQMLNLKHLEELENELDGKNRELTSKMMFLNQKNDLISRLMRRLQEIRDSDENS
                     SEEILSLVNELRSDSPQSSWKEFETQFIQVHPGFYQRLFEKHPALTSYEQRICAFLRM
                     NLNTKEISSITGRSAKSIEVARSRIRTKLELNRNENLSSYLAAI"
     gene            complement(11154..11795)
                     /locus_tag="PKZ74_03855"
     CDS             complement(11154..11795)
                     /locus_tag="PKZ74_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91922.1"
                     /translation="MLVFSWPSKAPDKSPIGNFKNSRINAVRSAPYLNEFIHLFNRLK
                     TEKESPVKFSIFFHSLANYMLESAIEQGFLDDVSPGLFENMILNAAATDSEGHRDWVE
                     RLNFQKRLFITSNDGDVNLAGLRAFSRFGYQLGEKPLEPLAQNATCLDFTQSVGFRLR
                     TGATHSYYYAFITRKSKNIRELFTQLLRGKEVNLSGHSRYQLLYAPNYYRVGF"
     gene            12125..13288
                     /locus_tag="PKZ74_03860"
     CDS             12125..13288
                     /locus_tag="PKZ74_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91923.1"
                     /translation="MQLVEVNDKKTSKQFLDVAKVLYKDDPVWVCPLDQDIIKIFDPA
                     KNTFYRDGNAIRWILKDDQGKLIGRIAAFYNKRKAFLYKQPTGGAGFFECINDRSAAG
                     MLFDAAKKWLESQGMEAMDAPINFGENDNFWGLLVEGYTHPAYGMPYNKPYYKDLFEE
                     YGFKPYFTQITKHLDLTVPFPDRFWKIAEWVNKRGGFVFKHFTYAESEKFLHDLKEVY
                     DQAWVHHEHFIPLDMGVVRRELESAKPILEEDFIWFVYHDDKPVAFYIMFPDINMILR
                     HFKGKLTLWNKLRFLYYKKNKEITRIRQTMMGVIPKFQGQGIESAIFWHLREPVLIKR
                     PHIKEMELSWVGDFNPKMMAIQEAVNAKPGKIHITYRKLFKEEAGEQHAAKVI"
     gene            13518..13955
                     /locus_tag="PKZ74_03865"
     CDS             13518..13955
                     /locus_tag="PKZ74_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91924.1"
                     /translation="MKRTVLFTLLVSLFIFVGSTISGAGAGEDGKWILKKSTGNVSLY
                     YKIGECNGSDVIFLKFDNKNNYNVRITWKETITDKANGAQIDNYKGQKELVLVPGVTQ
                     QDGCAGINAEECVIRMTEVTPVRVVDPQELEFKDVKVSKVSNN"
     gene            13982..>14965
                     /locus_tag="PKZ74_03870"
     CDS             13982..>14965
                     /locus_tag="PKZ74_03870"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HPM91925.1"
                     /translation="MKKIFQSLSSRLLTIMSVLLLVGAVSPSPVKAQTSQGTDFWICF
                     PANTSSGARELYITAGAAASVNIQIPGLGYSNLVNIPAGTLATVTLPGTVDVSSNGTA
                     DNKGIHITSDNPVTVYGMNQATATTDAFLALPVDALGTDYLVMGYTRDASFVLYAQMT
                     IVATQDNTSLTITPTATGGGFTAGVPGNVVLNQGQVFQLRSNSVGADYTGSHVVSDKP
                     VAVFGGNECTNISGSLRACDHLVEQMVPTSGWGRNFLTVPLATRLAGDVFRVMAQQAG
                     TQVSVNGSVVANLNYGQFHEMILASNTYNRITSNNPILVGQYSRSSDADGVT"
BASE COUNT         3713 a         3670 c         3486 g         4096 t
ORIGIN      
        1 ggaaataata aaaaccgggg ctgaccgggt tggagccacc accaaaattc gtattataaa
       61 ccagcaggct gttggccgga ttgggaatgg tccagatgtc gtaaacatcg gcaagtgaaa
      121 cacgggttat gagcagaccc aggttcgccg aatccagttc aaggatggca gaaggatcca
      181 tctggtcagg tttttcgccg atcttcactt gtgcagtcag agtgctgacc aggagtgaaa
      241 gaacgattac tccaaaaaac ttaagaattg gcatggtttt catgacacat aaattttttt
      301 taaatataag gaaatattag atgtttttat tggcatgggg atgataaagg ttgttggatt
      361 ttttgtttca tggctgattt ttttcaccac aataggcaca tagatcacat aggtttcaca
      421 taggattcta tgtgatttct atgtgtccta ctgtgcctat gtggtttgtt ttcataattt
      481 tatgatcttt ttcacgattg tttggttttc aagagatatc cggaggaagt agatgccggc
      541 cgggagatag ctgatgtcaa aatcaatagc agcattgttc aattgttcca ttgttatatt
      601 gttattggat tccacaatca atccacagat atctgtcaac tctaaacttt gaaactcaac
      661 tttgaacttt gaactttgaa ccttgaaatg ccctgtcgtt gggttcggcc agacctccac
      721 ctcctccctc tccatgtctc ccggcctccc tgtttcctct tccccattga tctgcgtgta
      781 cttgaaaatc tgcttccctt gccccgtaat atatccgcag gtgggcgaag tcatatgaac
      841 atcgatcaga ttttctgtcg tgccaaggtc cagtttctcc caggttccgc ctccattggc
      901 ggtaaagtac acctgcccga ctgtacctgc agcacaaccg gtttcggaat tggcaaaact
      961 gataccgaac agatctccgt ccagttgatc gccttccacc tgattgaccc agttgtaacc
     1021 atcttctgta tgcatgatcc ggtcgccgca ggtccatccg gtctgattat caatgaattc
     1081 tatatcgtta tgatagcctg taatatggat ctggtcggcg taccaggttc catctacata
     1141 atgcctgacg atattccagc cgctgagcca accctgtgcg ttgttcactg gaaaggagag
     1201 gccgtgaaga tcgctgttca cattcacatc tacgatatta ccgcctgttg gtgtgatcat
     1261 catcaggctt ccgttcattc cacaggcata cccggtatca gcaggcaatg gcggaaactc
     1321 aatttctgcc agggtagtcc caagtgtcca atggaagttc caggtatatc ctccgtccgt
     1381 tgttttgtat atgatccccc catcctgacc ccctgcaaag ccggtcaact gatctctcat
     1441 atgaatcgca ttgaaagcgg ttttgtgcgt ggaacggtgt tcccaggatt cgcctccatt
     1501 ggctgtgtgc aggataaaac tgctatctac ggcattatga atgacgatcc atccattcga
     1561 atcatccagg aaatggcttg cacgtatcat ggaaaccatg gtgtctccgc cgaaaatcac
     1621 cgtatcatag ggcagatcgg gaagatttgc tgaaatgttc ttccagctgt attgctgcgc
     1681 attcagtgaa cagtgaacgg tgaggagtga gcagagcagc agtgaagcgt gaagcgtaaa
     1741 gcgtgaagcg aatttctgac tttttggttt catggctgtt cttttttttc accacaattg
     1801 gcacatagat cacataggtt tcacatagga ttctatgtga tttctatgtg tcctactgtg
     1861 cctatgtggt ttgttttcat aattttataa tctttctcac gatcgtttgg ttttcaagat
     1921 gtatacagag gaagtagatg ccggccggac atccggtgag gtcaaattcc agggttccag
     1981 cccccacccc cggcccctcc cccatccggt ggaggggaga gattgcgacc ggtttaccga
     2041 ataaatcaac cacctcaacc tgcaattttg aatttggaat tttgaatttt gaatttgttt
     2101 ggtgcttggt gcttataatt tggaatttcc ccctggtggg gttgggaaac acctccacgc
     2161 ttcccttctc ccggtctccc tgtccccctg tctccggttc tccgttcagt tcggtgtact
     2221 tcagcaatgt cttctccccg ccgatgatat aaccattggt cggcgaagta aaaagtacgc
     2281 cggttaatat ttcatccgtc aggtgatgct ccagcatcgt ccaatcctct ccaccgtcat
     2341 tggaataggc gatttggccc tgagttccga cggcccatcc attgttcagg tctgtaaagt
     2401 aaacccccat catggcgccg gtctggacga attccggatc ggtccgatac cagtcaattc
     2461 ccacgatcgt tttcaggaac atttcaccga cgcaccaccc gaccgtatca ttgcggaaat
     2521 agagccagtc tttagatgaa aagggataac tggcctcgac atgccattcc tcatccagat
     2581 aatagatgat catctggtag tccaacaggg cataccccct ttcttccgaa ggaaaatcga
     2641 tgcaatacac actgccggcc aggccaagat caactgggaa cacgccttcg ggagagatct
     2701 gtgccaggtg gccgtctttc ccgcctgcaa aaccgttttg agccggccgg ggcgggaatc
     2761 ccaggtcgta caggggagcg gccaggagtc cgtgaagggt ccaggattgg gcgccgttcg
     2821 ttgtcttgta tataaatccg ttaattgcgc caaaataccc gttcagttca tcgatcatgt
     2881 gtatatcggc accggtatag ggtgcggaat gctcggtcca gttctgtccg ccgtcggtgg
     2941 tttcgagaac cgccgatggt tccccgtcga aggggtggta ggtactgatc cagccatggt
     3001 tgtcatccag gaaggatatc ccgctgatgt ttgcgatgag ggtgtcagcc ccgtcgttga
     3061 tgatcacggt gtcgttgtaa aagtcgggca ggttgccgga gatgtcgacc cagccgtact
     3121 gctgggcgtt cagtgagcag tgaacggtga ggagtgaaca gaggattagt gaacagtgaa
     3181 gggtgaggcg tgaagcgaat ttttgggttt ttggtttcat ggctgacttt tttcaccaca
     3241 ataggcacat agatcacata ggtttcacat aggattctat gtgatttcta tgtgtcctac
     3301 tgtgcctatg tggtttgttt tcataatttt ataatctttt tcacgatcgt ttggttttca
     3361 agattgatcc ggaggaagta gatcccggcc ggacatccgg tgaggtcaaa ttccagggtt
     3421 ccggccccca cccccgaccc ctcccccatc cggtggaggg gagaaatcct cacttccttt
     3481 ccggataaat caacaacctc aatcttttga atttgatttt tcgaatttgt ttggtgcttg
     3541 ggacttggtg cttggaattt gggatttgtt tggaatttgg tgcttgagat ttggaatttc
     3601 ccatgcgtgg ggttgggata cacctccacg ctttccctct tcaccttttc tccttcttca
     3661 tgctcttcga tccccgaact ctccgaataa aaatagtagt ccctgaaact cagcaaatac
     3721 tccccggtgt tcacgacgag cttgtaatac gagtcggtgt gcatcctgaa cagggaatca
     3781 taccggtagt tatgacagtc cacgaactcc cacacctggt cgtcttcaat ccattggaaa
     3841 agtgaatcag ccatgagcaa ggtgtcgttc tggtatggat accgccgctc ataggtcggc
     3901 acccactgcg cctggtcatt cccgctgtat cccctgtaat ggatcaacct gccccggttg
     3961 ttataggtca tttcagtttt taaattcgga taccaggtta ccaggtcttc cccgatccgg
     4021 taataagcgt aaatcgtcag gctgtcgtta tcgtcccagc cccagtcctc tttctcttcc
     4081 tcaacccaca tttccccctg ttcatcatat aaggaataca ccgaatggtt tttcctgttc
     4141 tgctcgtcat aattatactg aaaccgtatc gaccaatacc attgttcggt gttgggatta
     4201 taaatttgat gggtgatttt gaagacattg ccaaattcat cgtactcata atctcccctg
     4261 tcactgggat aaatgatccc tgatccttca tcgggctcat aggtggtcag gtactccagg
     4321 aaacctgaac cgctatattc atatatttcg tatgaagcag tatcccaata ttcagaaacc
     4381 gtattccact ccagatgatc cctgcgggtc agcttatttt cattatcgta atcaaaaagg
     4441 tcccagaaac ggtttcccca gtcatacgat gtatagtccc aggtgaaata atgatattct
     4501 gcctggtttc cgttattgtc atacattgtc gtttctttca tccatccttt ataagccatc
     4561 tcattgttat cccactcata aatggcccaa agggtgcgcc ggtcgtgatt atcatattcc
     4621 cgtacctctt tccggttggg gatccagaga ttcagggatt gttccctgac aaacttttcc
     4681 gattcgataa cacgcaaatg attgtcatag cggtaggtca tctttgcgtt caatgtcgaa
     4741 tcgccctgcg cgtcgaactt gtaggtgatg accgaatccg gggaggaggg ggccagggtt
     4801 tcacggcccg gtggctgacc gtatcccatt gaaaaaaggt gaatgaggat aatcaggggg
     4861 aggaaggtaa tgttcttcat gatcaataat ttttaaggat gatgaatttt ccggtgaaga
     4921 tttcgttacc ggctatgatc cggcagttat agagtccatt cctcatgtgc atattattca
     4981 attcgaattg accgtcacct tcggggaagt accgtggctc acttttgtag acgatacggc
     5041 cggcgatgtc ggtgatttcc acgacggcat gttcctggga ggaaagtgta aatccgatga
     5101 tagcctggtc gtttacaggg tttggtgaaa cgatggcagg ttgggtttta tgattagtgt
     5161 tgataaaagg taattctttg ataccgagcg gaggcagcgg cagcatggta agggtaccgc
     5221 ctttgcggta aagaactata ttccaattgt ttgcattccc tgcgatgttt cctttgccaa
     5281 ctgccacgta accgccgccg gatgtcggct gggcatcatt ggccagggcg gtagtaggat
     5341 ataatacata tccgggcgac cagcccatgg tatcggaaga gatgaaattc ccttcggtat
     5401 cataggtcct gacgttcatc aggtattgtg agccgaaccc gttccggctc agcaaaatat
     5461 tgccgtccgg ctttttgctg atagtcgtga acccaccatt ctgctctgtt tcactggttt
     5521 tctcccaaag agtttttcca tcggcatcca gtttggctat atactcttta ttgagataat
     5581 aacccaccag gagaaaggtt ccgtcgtcct gttccaggat atccccgcct gcatcccagt
     5641 aatcttcgga gccgatctca cgggtccaga tcgtatcgcc tgcggcatcg aatcggatcg
     5701 cataaatatc ggtaatgtag cccagggtga gggcatgccc ccgggtgccg gcgaaaatgt
     5761 atccgccatc ggctgtacgg tgcacctcac cgacctcatc gcgtcggtcg ccgccatatc
     5821 tttttgtcca caggctgtcg ccctgcgcat tgatcttcat gaaccacaaa tcgcccgctc
     5881 catcgtactg gtaggtcttc ccggcaatga agtaaccccc gtcggtggcg gtgacaatgg
     5941 aatagccttc ctcgtcggtt tcgatgccgt aggtcttttc ccattgcata ttgccgttct
     6001 catcggttct gacaatgtaa aggtcttttc ctccggctcc aaatgatttg gtgaaaccgg
     6061 tgaggatata tccgttgtcc gggcagatgg ccacatcgaa cgccctttct tcattattgc
     6121 cgccgtaatt gcgggtccag agggtatcgc cgttgatatc ggttttaatg agccgcatgt
     6181 tataggtgga ggaagtcatg gaagaaccct ggtatcctgc aatgaggaag cctttgtcgg
     6241 gcgtttccag cacggagtgg ccttcgcccc aggtggcgta accttcgagg ttgtagaatc
     6301 cgtcggtgtt tttcgggggt tgcagtttcc atgcatacac atcatggagc cggttggcct
     6361 tgaagtcata ccctgtcaca tagaggtacc cgtcgtcgtc ggaggtgatg gcataggcaa
     6421 catccatatc gatcgcaggg cccacgtaac gcatgaccca gaggtcgcgg ccggtgcggt
     6481 ggtcaagact gatggtgccg gcttcggcga gcaggccgca gaagtagccg gcgacatgca
     6541 cgttggtgtc gggcgtctgg gtgatggaaa aggccatgtc atgatcgccg tacaatcctt
     6601 catagcggta gaaccagacg gggctgccct gaaggtccac ctttatcact gcaacatcca
     6661 tatcggtatc cacctggttg tcgcggccgc cggcacgccc ggtgacatag atgttgttat
     6721 cgaagccata gatcagctca tagcaggttt ctccccagta tggctcgggc ccggggccgt
     6781 cgacattgca gttccactgg tactggccgg tggcgtcaac gcaaatcacg gcgatgttcg
     6841 aatagatcag gccgctggcg tagatccggc cgtcgtcccc ataaacgatg tccgttgccc
     6901 aggtcctgcc ttccgaaaat gggaacaagt atttccagtt cttgcttcct tctggtgtta
     6961 ggctgaggat ggtgagttgt ggttcggtgg cctcatgagt gtagcccgct gcataaatat
     7021 ttccatcatt acccacggtg atggccgaag cctggtcgtc gcataaagca tacgggcacc
     7081 cgccgtcgaa ccagtagatc cattcctgct ccccactccc ggcattgatc ccggccacaa
     7141 ggaaatcata gttggccttc ccggccgcat aaaccatccc gttgccgccg tatgcgacag
     7201 aaaacgcctc gctgggatag tttccatcca catcataaat gtattcccat cgcaagcctc
     7261 cttccggatt tatgctgatc accaggaatt tattattgtc ctccgcgtta ttggcagtcc
     7321 cgcatacata gatgttgccg tcgctgcctg cctggatctc ggcaactacc atggttttat
     7381 ccggttggcc ttcgtagctg taaacccatt gctggttacc acttttgtct aatttgatca
     7441 cgatgatgtc ataatcagct tcatcgtatt cagtaccggc aatataaatg tatccatcgg
     7501 aaccacagac gatatcttta ccttcctcac ttttgccagt gttgtaatta taataccatt
     7561 tgtggttctg gcccgtggct aagagcacag ggcatagggc taagaacagc agtgaagcgt
     7621 gaagcgtaaa gcgtgaagcg aatttctgac tttttggttt catggccgat attttatgtt
     7681 ttgatcattt cctttttcac cgacataatc ctgttcaggt tgctcataaa taccatgggt
     7741 gtcaccggta aaacgcctct gtcataccat tccgggaaaa ccggcggcaa cccgttcact
     7801 ttttcaagca atgaataaaa gtttaccggc atcatgaaat cgaagcggag tttattgata
     7861 caattctgcc tgactgtttc aagcgttgct tctttaagcc ccagcaggtt tgctgccgtc
     7921 cgtgcaaata agctgtcggt gacaggttct tccaggaaag gcctgataaa accggatgcc
     7981 cttccaatgc cggcagcatc agcattcaga acaatcatct ccgccagcat gtccaccggg
     8041 atgatccgga aactgaacgg gaaaaaataa cccgtcttca gaaaaatatc caggatgatc
     8101 cagatggagt agtgcgacgg gcagatgccc tttcgctcag aaccaagcac cagcgggaca
     8161 tcgtaaatac atgaaccgag gccctgccgt gaggcattcc ggatggcttc ctgcgctacc
     8221 catttgaacc cggcataacc cgattgccag gcattcctgt tcaaggactg aaaatcctca
     8281 tcggtcacaa aaaaattagc gttataagaa gaagtatagt gcagaaattt gggatattct
     8341 ttgtccatgc aaaaggaaat catccgctca agctccggaa tccatgtttg ctggaaatga
     8401 tgatagggca ggtggtacat cggggtcata gccagatgga ttacctgtcg tgttttcctg
     8461 cacattcgcc tgtactcttc cggattcatt cccattccgc tcaaacatac atcaccccga
     8521 tggatgttaa aacgacggat attgaactgc cccagttccc accggtcgaa ggattgctgc
     8581 atccgggcga cggcttcaaa atcattgggc gcacggacaa ggagatggat atctccctgg
     8641 aagttctgct gtaaagcttt gataacatgg catcccagaa aggagttggc tccggtcacc
     8701 agtatccctc ccgaaaagtt ctttttaaca ggcaaagcat tctcccagtt gcggaatatc
     8761 agtttttcat tgcggatgtc ttcagtaaga ggagtcatgt cagatgattt gattggctgt
     8821 tataaagata caaccgccag aaaggtcaat gcaaatattt caggttacgt ttccactcac
     8881 ggcaccttac aaaaacactc gcctgaggtt gtctcaaaag tctatatatt cacttttttc
     8941 aggtagtccg acgactgaag ccgtcggttc aaaccttcta agattcccag gaaccaacag
     9001 ctttagtcgt tggagcacgt cctgttttca ggcaatcaaa tcgaaatatc agacttttga
     9061 aacagcctcc ttacaaagtt gggacccgtt agttcaagga tcaaatagct gccaggtaac
     9121 tgctaaggtt ttcgttcctg ttcagttcaa gttttgtgcg gatgcggctt cgggccacct
     9181 cgatactttt ggcggatctt ccggttattg aagagatctc ctttgtattc aggttcatcc
     9241 tcagaaaagc acaaatgcgt tgttcatacg aagtcagcgc cggatgcttc tcaaacaaac
     9301 gctggtaaaa tcccggatgc acctggatga actgcgtttc gaattctttc cagctgcttt
     9361 gaggtgagtc ggaacgaagt tcattaacca gcgacaggat ctcttccgat gaattttcat
     9421 cagaatcgcg aatctcctgc aaccgtcgca taagcctgga gatcaaatca ttcttctggt
     9481 taaggaacat catttttgag gttagttccc tgtttttacc atccagctca ttctccagtt
     9541 cttcaagatg tttcagattc aacatctgct gctgctgaaa aagtaaccgg gcatcccggc
     9601 tccttctaac caacaagata ataattataa tgctgaatat caatcctgta acgaaagcaa
     9661 tgccgactat tgtcctgctt ctcagttttt gctcctgcaa agtggccttg ttccggagaa
     9721 tctcattttc tttttctttt ttatcgtaat tatattttgc ctcgatccgg gtgatctctt
     9781 tatgctgatt ggcgaaattc aggctatcgg ataatttctg atactgtttt tgccagaata
     9841 gcgcttcacc cggttgacca ttcttttcat aagcctgtga taaaaccatt gccacatctt
     9901 tgctgccggc atgaatcatg agttcttccg ccatgtcgaa tgcttcccgt gccgtccgga
     9961 tggcttccga atatttccca tgttccagta aatttctgga actccaaagc aaaacattga
    10021 tcatcattgg tttatcattg gtttttttga ccgattccgc cgccgccgct aaaaattccc
    10081 gggctttatc gtaatttcct tccaggtagt aaaactttcc cagtaaaagg ttgcattcgg
    10141 taatgaatgg ctgggctccg atctcacttg ctacggataa agtcttgttg cagtattcaa
    10201 aagcggtctc attccgtttc atctccatgt aaagatcagc caggttggaa taccggatca
    10261 ccagctgcga tttttgctgc aatgcttctg atatggcggc agatctcagg atataatctt
    10321 ccgctttttc aaattccccc agcatcttca tgaccacgcc tatattcaat aaagccagtg
    10381 ataccgcttc ccggttgttc tttttatcat aataatcaag cgctgtttga aaaagtcgcg
    10441 tcccctcctc caggttcccc agctcacaca gcaaaatgcc gttattaagc tgaacatcag
    10501 caatctgggt actatccccg atagctgcag ccaaagcaga tgcccgccgg taacaactgt
    10561 ccgattgttc caggttaccc aggtgccgtt cggccagtgc ccaggcctgg atagcatcga
    10621 acatgccctg gttgttatcc gactcgtaaa acatttcata cgactcctta aagtattcag
    10681 ctgccttttt ataagaattc gtcacataaa acgtataccc cacatcgtaa agggaattgg
    10741 cggtaaaatc gctgaatccg gctttcctgg ccagtgtaag tgaccggcgg taataataat
    10801 cggcccggtt gtactcatca ttataataat aaatgtttcc tatgtaattc tgggccctga
    10861 tcacagatac caggtaagct ttttccaaat cgctgattcc ggggtgaatg agattcgtgt
    10921 cgaccttcgt atggcttaat atattttcat aaaagaagac agcagaatcg ggattggaat
    10981 aatgaatgct ccgggctttt cgcaacagat ggttcaccct tgccgtatcc tgctcagtga
    11041 tcgtcgctga aaaaccggat accacggcaa ataccaataa aatggcccat tttgaaaagg
    11101 atttcacaaa gcgcagctct cttgattatg acaaataaaa atacggattt ttattaaaat
    11161 ccaacacgat aataattcgg agcatacaaa agctgatacc ttgaatggcc ggaaagattc
    11221 acttctttgc cccgaagcaa ctgtgtgaac aattcgcgga tgtttttact tttccgggta
    11281 ataaaagcat aataataaga atgtgttgcc ccggttcgca ggcggaaacc aaccgattgg
    11341 gtaaaatcca ggcaggtggc attctgagcc aggggctcca gcggcttctc cccgagctgg
    11401 taaccgaatc gggaaaaggc cctcagtcct gcaagattga catctccatc gttgctggta
    11461 ataaacagcc gcttttgaaa attcagtctt tccacccagt cccggtgccc ttcactgtcg
    11521 gttgccgccg cattcaggat catattttca aataagcccg ggctgacatc atccagaaat
    11581 ccttgttcaa ttgcgctttc cagcatataa tttgccaggc tgtgaaagaa aatactgaac
    11641 ttaaccggtg attctttctc agtttttaac cggttgaata aatggatgaa ctcattcagg
    11701 tatggagcag accggacagc attaattctg gaatttttga aattaccgat cgggctttta
    11761 tccggggctt tcgacggcca gctgaaaacc agcatgttta cctggtgaag ccgctgaatc
    11821 tcaagtgccc gctcaaccga aatcaggaat gactgcccgt caccgttgta tccacgatcc
    11881 tgtttgtgac cacgaacact ttgttgtcag cccctgtaag ggaatgatta ttggaaaatg
    11941 cctgtccata agatgttgaa catgacaaac ctgacatcat ccacaagaag aaattgaata
    12001 ttaaacagga gacggaggca ctccccaata gattaaacct tatcatcccg attattcttg
    12061 acccaatcaa accggtaatt tttttcaaat ataaaacgta attttacgcg ctaaatagtt
    12121 ccccatgcaa ctggttgaag ttaatgataa aaagacatcg aagcagtttc tcgatgtggc
    12181 taaagtattg tacaaagatg atccggtttg ggtttgcccg ctcgaccagg acatcattaa
    12241 gatctttgat ccggcgaaaa acacttttta tcgtgacggg aacgctatcc gttggatttt
    12301 aaaagatgat cagggaaaac taattggccg tatcgccgct ttctataaca agcggaaggc
    12361 ttttttatac aaacaaccga ccgggggggc aggttttttt gaatgtatta acgaccggtc
    12421 ggcggccgga atgctgttcg atgcggcaaa gaagtggctg gaaagccagg gaatggaggc
    12481 gatggatgcg ccgatcaatt tcggggagaa tgataatttc tggggactgc tggtggaagg
    12541 ttacactcac ccggcctatg gcatgcctta caataaacct tattacaagg accttttcga
    12601 ggaatatgga ttcaagccct attttaccca gatcaccaaa cacctcgatt taacggtccc
    12661 atttcccgac cggttctgga aaatagccga atgggtcaac aaacggggcg gtttcgtctt
    12721 taaacatttt acttacgccg agtcagaaaa attcctccat gacctgaaag aggtttatga
    12781 ccaggcctgg gtgcatcatg aacattttat cccgctagac atgggtgtgg taaggcggga
    12841 actggagagc gcaaagccta tcctggaaga agatttcatc tggtttgttt atcatgatga
    12901 caagccggtg gccttttata tcatgttccc cgatattaat atgatcctga ggcatttcaa
    12961 aggcaaactg accctctgga acaagctgcg ttttctttac tataagaaaa acaaagagat
    13021 tacacgcatc cgccagacga tgatgggcgt catccccaaa ttccagggcc agggcattga
    13081 gtcggctatt ttctggcacc tgcgggaacc cgtcctgatc aaacgtccgc atatcaaaga
    13141 gatggagcta tcctgggtgg gtgatttcaa cccgaagatg atggctatcc aggaggcggt
    13201 caatgccaaa ccggggaaga ttcatatcac ctaccgcaaa ctgttcaaag aagaggccgg
    13261 cgagcagcac gcggcaaaag tcatttaaaa aatcaatgtg tcttattaag acaaggttta
    13321 taaagaatgt cttaaaaaaa ttcgaatcaa tcttctcgat tacagcgatt taacaaatct
    13381 ttaacattcg gcatgtacga ataaattatt cgtacatctt tatcatccag acgtacaaga
    13441 atagctttaa ttgatattga ctaatccaga tacgtcaatt agatttgtgt caaaatccta
    13501 acttaaaatt tgatattatg aaaagaacag tattatttac actcctggtc tccctcttca
    13561 tctttgtggg cagcaccatc tcaggggccg gcgccggcga agacggcaaa tggatattga
    13621 aaaaatccac cggtaacgtc agtctttatt acaaaatagg tgaatgcaat ggcagtgatg
    13681 tcatttttct gaaattcgat aacaagaata actacaatgt caggataacc tggaaagaga
    13741 ccataacaga taaagctaac ggggcacaga ttgataatta taaaggacag aaggagcttg
    13801 tccttgttcc gggtgtgacc cagcaggatg gttgtgccgg tataaacgct gaagaatgcg
    13861 tgatccggat gacggaagtc accccggtcc gggttgttga cccgcaggag ctggaattca
    13921 aagatgtgaa ggtcagcaaa gtatctaata attaatccac taattcttaa atcaatccat
    13981 tatgaaaaag atattccaat ccctttcaag ccggttgctg accatcatga gcgttcttct
    14041 tctggttggt gcggtctcac cttctcctgt gaaagcccag accagccagg gcacagattt
    14101 ctggatctgt ttccctgcaa ataccagttc aggtgcacgc gaattataca tcacagccgg
    14161 agcggctgct tcggtgaata ttcagatccc cggtttaggc tactccaacc tggtcaacat
    14221 tccggccggt acgctggcaa cggtcaccct ccccggaacc gttgatgtat caagcaatgg
    14281 aactgccgat aacaaaggaa tccatattac atccgataac ccggttaccg tttacggaat
    14341 gaaccaggcc acggccacta ccgatgcttt tcttgccctg ccagttgatg cattgggcac
    14401 cgattacctc gtgatgggct acacccgcga tgcatccttc gttctctacg cccagatgac
    14461 gattgtcgct acgcaggaca atacctcact gactattacg cctactgcaa ccggcggcgg
    14521 atttacggcc ggcgtgccgg gaaatgttgt cctgaaccag ggacaggtat tccagctcag
    14581 gtcgaattcg gtcggggcag actacaccgg ctcacatgtg gtatccgata agcctgttgc
    14641 cgttttcggc ggaaatgagt gcaccaatat atcaggaagt ctcagagctt gtgaccacct
    14701 ggtggagcag atggtaccca catcgggctg gggcaggaac ttcctgaccg ttcccctggc
    14761 aacccggctg gccggagatg ttttcagggt tatggcgcag caggccggaa cccaggtcag
    14821 tgtaaacggt tccgttgttg ctaacctgaa ttacggtcag ttccacgaaa tgatcctcgc
    14881 cagcaacacc tacaaccgga tcacctccaa taatcccatc ctggtagggc aatactcacg
    14941 gagctcggat gccgacggcg taacc
//
LOCUS       DAOPNH010000046        14103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4375_length_14103_cov_6.054812, whole genome shotgun sequence.
ACCESSION   DAOPNH010000046 DAOPNH010000000
VERSION     DAOPNH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4375_length_14103_cov_6.054812"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKZ74_03875"
     CDS             complement(<1..228)
                     /locus_tag="PKZ74_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91926.1"
                     /translation="MITSPGFTQETPNPSTISISVFPNPFSDQVKITVNAEKGADLSV
                     VVYDILGRKVKGWRPEGWRPFFDDSGLECPAG"
     gene            582..3803
                     /locus_tag="PKZ74_03880"
     CDS             582..3803
                     /locus_tag="PKZ74_03880"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM91927.1"
                     /translation="MKSLLSASLACLFMLSAYSQTNHAPVAVSDTVEVVSQISTIIDV
                     LANDYDPDGDSLIISYVNFPFYAQEVEIINNKIRFKSDNLIEKVLLTYRISDDNQPPL
                     SSNSTYVHIYLLKNPNCPVANFEYYNLEEMKPIELDVMGNDEDPDGDPIKLYEIPETH
                     NCNVSINADSTRVIVKPGYMANRLGSFEYVIREVNTPDNYLSLPMTSHFKIVQNPQIP
                     VLFPDTANVTGGIPFTIPVLENDYDLQNDPFEVFHYYNPYTGTVTQVGDDLVYTAPVS
                     FAGKDSFWYDAREIDDTNIVTNKQKVIISVEKNPLCPVGVPDHVSGKCYEAFTIDAIA
                     NDYDPEGYPVVIFEVQGGGEVVIDNNKIIYTSNPRVINHDTIYYRIRRSTNHEYYSEL
                     TPIFIDLEQNPVLPVAVDDYARATIFAPVIISPMDNDIPNAADSLILVWPTSDIGYVQ
                     RISDSLLSFQPSTASSGQEIVSYYILDKNNYNMVARGRIFVDIEPHHFFDTLNINNIK
                     AWVNADGLLFNKAREIPGMKSYPLLMSYFRYPANQETSTIFNSLIWIGGVSDSGNLHF
                     AGERYRFEGTDFQPGPVSDVYDSTYARKYWRLWKLNREDIEYHRYNWWKSGYQPVDAI
                     VTWPGNGITEAGQADRLAPFKDIGQDGRYDPMEGDYPLIRGDQAIYFIKNDDKIHTES
                     DGTRLRLEVHGMVYGFNAPGDSALMHTVFVHYDLINRSENIYYETYAGIWTEIDIGGM
                     WDDYIGSDVGRNTYFGYNGDDFDEDDTEQPEEITKGYGKNPPAQSVTILAGPVIDADG
                     TDNPPGGCDYGVNGINFGNDMVDDERYGLCSFMNNIQLPAHVGNPYYPAPILYNILKS
                     QWYDGQDLLFGGDGTIYDSRAVGPGCRYVFPGSSDILNLGTGCLPPEEPYNTGGFYWT
                     DSNMNSPGDRRGIGSMGPFTFKPGDVQEIELAYVVANGWNGPVSSVDKLMEYIDTLRA
                     RVQNGGLIVPNSELGYNEPSKIPGSLKIYPNPASDFVVVEIKGIESGCEYEIYDLFGR
                     MVLSGILSFQAQNVINTNGLSSGLYVLMVKTDDMVYSGKIIRR"
     gene            complement(3876..7325)
                     /locus_tag="PKZ74_03885"
     CDS             complement(3876..7325)
                     /locus_tag="PKZ74_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM91928.1"
                     /translation="MNENASGNPANYLQLKAQNIERLKELAAINRTTQIIKEGKSIEE
                     TLKQISTILPPAWQYPQFTAARILYEGKEFASIEFKATPWVQRQRFETIDNKVGYIEV
                     YYLKEFPEIDEGPFMIEERHLIENLANMITGFINGQLAKTIIKPGAGRTAAKSDDGQP
                     VVTSRQLLQRFINKSNEDRDIYHDLMPFKVKEILLVANLYDAYSIEKEGRFSEHVLGE
                     YHTLNLTSIPRITGVSGIDEAMDILGQRHFDLIIMMMGADKRTPIGLSCKVKRLYPYI
                     PVFLLLNNNAEMALISEDKNALEHIDKVFIWNGESSVFFAMIKHVEDKINADNDTDIG
                     MVRVILLVEDSAKYYSRYLPMLYHIVLEQTKRIIEDVSTDELYKILRLRARPKILMVS
                     TYEEATAVMERFRDYLLCLITDVKFNRNGVLDERAGFDLVEYSRSVHKDMPIIIQSSE
                     EENSEMAYRLKCTFINKNSETLSQDFRSFITHFLGFGNFIYRNEQGAKIAEARTLREF
                     ERLLRVIPDESLLYHAKRDHFSLWLTARGEIQAAKILNPQKVTDFENARQIRDYLIDV
                     IGRFRNEQNQGKIIPFEESAITDETNIVSLMDGYLGGKGRGLAFVNTLIYNFDFSKQI
                     PTIKIQAPKTSVIGTEEFEYFMDRNHLRDFVASTTDYEEIKKRFMDAKLTDTLVKRMK
                     VLLTRITKPIAVRSSGLFEDSLMQPFAGIFETYLLPNCHPDPNERLKQALDAVKLVFA
                     SIYSPEARGYIEAVHYKIEEEKMAVVIQEVVGRQYGNYFYPHISGVAQSYNFYPYAHM
                     QPEEGFAVTAVGLGKSVVDGEKAYRFSPKYPTTEILSPKDQSQNTQVEFYAVDLNKKE
                     INLLEGDTAGLAKLDIYEAEMHGNLKHCASVYDADNQVIIPGLTHPGPRLINFANILK
                     YNFVPLAQTIEVVLDVVKEAMGSPVEIEFAVDLTKDNHGRATFYLLQIKPLIGNAMDY
                     NVNIDKIPKEKIILYAEKSMGNGVIDNIEDVIFVDIDTFDKGETMQMTYEIEEMNRKF
                     AAENKKYLLIGPGRWGTRDRWIGIPVNWAQISHARVIIETSLEGYPLDASSGSHFFHN
                     VVSMNVGYFSIQPELSKSYIRWEILKQQPVISQTKYFRHIRFDKPLTVSMDGKKRIAV
                     VTV"
     gene            7443..>7982
                     /locus_tag="PKZ74_03890"
     CDS             7443..>7982
                     /locus_tag="PKZ74_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91929.1"
                     /translation="MRLIIILLLILTLPFSPAGQTADEVLSRYFVTELEGNVFMRWTI
                     TAGNTCEDTYIERSADGIAFERIGLIAGICGSPDASITFEFTDTVPVYNQISYYRLML
                     GYFGYTKPLAIEVKKFNDEGFFAGPNPFRDFTRIAFRNDDNEEYRLLVCDMQGRSVLQ
                     MLTNGNEFIIRREELGSGIY"
     assembly_gap    7983..8082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8083..8580
                     /locus_tag="PKZ74_03895"
     CDS             <8083..8580
                     /locus_tag="PKZ74_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HPM91930.1"
                     /translation="MESRPKIKIGQTTTDRFVEIAGWAALAFMWIITLYYFRKLPETI
                     PTHFNMKGTADGFGQKSTIFLLPAIGTVLFIGMTILNRFPHIFNYPVRINSENAIRQY
                     TLATRLLRIFKVSIVLIFLMITWMTSAAANNQGKNSFAWFLPVMLAITFVPLGIYIFK
                     SFRAK"
     gene            complement(8746..9201)
                     /locus_tag="PKZ74_03900"
     CDS             complement(8746..9201)
                     /locus_tag="PKZ74_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPM91931.1"
                     /translation="MKEFENIEAILAFAIEQEQKAVDFYTGLAKEAHSEEMKKVFLEF
                     AGEEIRHKKRLTQISEQGLFTMPIEKVADLKIADYTVEVNVSGALSYEQALVLAMKRE
                     KAAFRLYSNLAERAPNQELKDIFLSLAMEESKHKLRFELEYDEFVLREN"
     gene            complement(9188..12910)
                     /locus_tag="PKZ74_03905"
     CDS             complement(9188..12910)
                     /locus_tag="PKZ74_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPM91932.1"
                     /translation="MSEKVNIILNGNIVEGSRGETILELAKRNGIEIPTLCNDPRLEP
                     FTSCFVCVVEIEGMKGLQPSCSTRLYEGMKITTDNEKIHKARKTALDLLVSNHYADCI
                     GPCKQTCPAGVDVQGYISLIEKGKYHDAVALIKETNPLPAICGRVCVRPCEVACRRNL
                     LDEGAAVGIDYLKRFASDIDIDSPDKWRPEIKPASGKKVAVIGAGPAGLSCGFFLQKE
                     GHQVDIFEASPRAGGWLRYGIPEYRLPNDLLQKEVDNITEMGVNIFYNKKFGNDISYK
                     DIKNQYDAMILTIGSQKGTLIGCEGEDAEGVFSGIDFLKEMEMTGQRFNFRGKKVAVI
                     GGGNTAMDCCRTSIRCGADKVYVIYRRTEKEMPANPIEIHESKLEGVEYMFLTLPVKV
                     VKSEAGGVKSITCVKMELGEPDASGRRRPVPVPGSEFDVEIDYILAAIGQKTEADFLD
                     DIKSYATDGEVKANRWGDLEANPDTLQTGIPSVFAAGDGVTGPATLIQAIAQARKASH
                     SCDLYLKGLPVVPITKEFYSSKDVFKPQSNTDYHGLFTERKRHEMPTLDPKDRLNFKE
                     VELGYESEEVAFRETQRCLECGCTALFTCDLKKFSTEYNADQKAQAGEFKEYRVDFRH
                     PYIEIDNNKCILCSRCIRICREVVGAGALGLVNRGFDTYVAPSMGNALYETTCESCGM
                     CISTCPTGAITENVPFKPGPVRLQPAETICNYCSVGCSISLDHKSRFVMHVSGVEGQV
                     NSDGNLCRYPRFGYQYLNDRSRITSPLLKVNGKFEEVTFEKAYEVIRQKIHSTGADDN
                     AFFAGARLSNEELYLVQKLARAGVKTNNVTSFHYLGRGAGYRNNTTAATPFSQIRDAG
                     RVFLIGSEISRDNAVAGFNIYNAQYIKGVKVELVTTKDNSAEAHKADSVLVIKSYYDF
                     IKAINFYLVHNNLHNNLFIRDNCKGFDDYKDELLKESFTDLVQRSGVSAEEIVEFATR
                     YNEEQRAVIVFSEKELSSNACTELQNLSLITGKLGKTGSGLIALKEKNNSQGLHDMGI
                     CRNYGAGGVSLHDAESRSRIQKAWKMADFPAINPVPIVERLESGTLKNLFIFGEDPAG
                     CTFDKAKIHGWLKQAGFIVVQDYFMTETTALADLILPASFPVESGGSFTNTQKVIQEF
                     EAHFTPVSRTTLEQLSDLLKAEGIDQSADYHDVLNEIVSLLPEPGKESFHLFTVTNQD
                     NQARLFEHGCDYLVKRFDSEFTEAFENVKTEVYERV"
     gene            complement(13075..>14103)
                     /locus_tag="PKZ74_03910"
     CDS             complement(13075..>14103)
                     /locus_tag="PKZ74_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM91933.1"
                     /translation="DEGRFFAAYEGAGALFDIYIKEGAGAFVCGEETALIASVEGERG
                     MPRKRPPFPAVSGLWRKPTNINNVETYANIPWIILNGPYAYAKYGTEKSKGTKVFALA
                     GKIKRSGLVEVPMGITIRDIIFKLGGGIQKDRKFKAVQMGGPSGGCIPDYLADTIVDY
                     DSVNATGAIMGSGGMVVMDEDTCMIDVAKFFLDFTQKESCGKCTFCRVGTKRMLEILQ
                     RISEGKGEEGDIERLEELAYQIKDNSLCGLGQTAPNPVLTTIRYFRHEYEAHIRDKKC
                     PAKACKSLLTYEVIGEKCTGCTVCAKNCPVNAIDGERKTVHYIRQEDCIKCGMCYTKC
                     KFEAIKLS"
BASE COUNT         3308 a         3498 c         3405 g         3792 t
ORIGIN      
        1 tcccgccggg cattcgaggc cggaatcgtc aaaaaaggga cgccatccct cgggacgcca
       61 tccctttact ttcctgccta gaatatcata aaccacaacg ctcaagtctg ctcccttttc
      121 agcattgaca gtaatcttaa cctggtcaga aaacggattg gggaaaacgg aaatgctgat
      181 ggttgaaggg tttggagttt cctgggtaaa acccgggctg gtaatcaaaa tgatgtggat
      241 tatggttaaa aatataaggc attttctttt catttgtttg gctttgaatg gctaaagtta
      301 atcatatttt gttcagactt atatgaaaaa caaaaagaaa atcaattgtt taaaaagggt
      361 tccaaagatg ttcgcatttt gcgttttgca ttttgcattt ttcatttcat tattcatttt
      421 gcatttcatt ttgcatttca ttttgcattt ttttagggat tttttacctg attcggaaaa
      481 gttttgatac cgatggcttt atggattatg ccggattctt tgttttgctt gacttttgca
      541 ggtttgtact ttacatttaa tccgccaaaa tacttaaacc gatgaagagc ctgctttccg
      601 catccctggc atgcttgttc atgctcagcg cctattctca gaccaaccat gcgccggtcg
      661 ctgtcagtga taccgttgaa gtcgtgtcgc agatatcaac cattattgat gtgctggcca
      721 atgattatga tccggatggg gattcgctta taatctctta tgtcaatttc cctttttatg
      781 cccaggaagt agaaataatc aataataaaa tccggtttaa atcggataac ctgattgaga
      841 aagtcctttt gacgtatcgg atttcagatg ataatcagcc gccacttagt tcaaattcga
      901 cttatgtgca tatatattta ttaaaaaatc caaactgccc ggtcgcaaac tttgagtatt
      961 acaacctgga agaaatgaaa ccaattgagc tggatgttat ggggaatgat gaagatcctg
     1021 atggagatcc gataaagctt tacgagatcc cggaaacaca caactgcaat gtatcgatca
     1081 atgccgattc aaccagggtc atcgtgaagc ccggatatat ggcaaatcgg ttagggtcat
     1141 tcgaatatgt gatccgtgaa gtcaatacac cggataatta tttgtcttta cccatgactt
     1201 cccattttaa aatagttcaa aatccgcaaa ttcccgtact ttttccggat acggctaacg
     1261 taaccggagg aatccccttc acaattcctg ttctggaaaa cgattatgat ctgcaaaacg
     1321 acccattcga ggtttttcat tattacaatc cttatacagg cacagttacg caggttggcg
     1381 atgaccttgt ctacactgct ccggtatcat ttgccggcaa agattcattt tggtacgatg
     1441 ccagggaaat agatgatact aacatcgtaa ccaacaaaca aaaggtaatc attagtgttg
     1501 aaaaaaatcc attatgcccg gttggagttc ccgatcatgt gagcgggaaa tgttacgagg
     1561 ccttcaccat tgatgccatc gccaatgatt atgatccgga agggtatccg gtcgtgattt
     1621 ttgaggttca aggtggaggt gaagtggtta tcgacaacaa taaaatcatc tacacttcaa
     1681 atcccagggt gatcaaccat gatacaatct attaccggat ccggcgttca actaatcatg
     1741 agtattattc ggagctgact cccattttta tcgatctgga gcaaaatcct gtgcttccag
     1801 ttgcagtcga tgattatgcc cgggcaacga tctttgctcc tgttatcata tcacctatgg
     1861 ataacgatat acctaatgct gccgattctc ttatcctggt atggccgact tccgatatcg
     1921 gatatgttca gcggatatcc gacagcctgc tctcttttca accttcaact gcaagttcag
     1981 gccaggagat cgttagctac tatatcctgg ataagaacaa ttataatatg gttgccagag
     2041 gccggatttt tgtcgacatc gaacctcatc atttttttga tacgttaaac atcaataata
     2101 taaaagcctg ggtcaatgcc gatggcctat tgtttaataa agcacgagag attccgggaa
     2161 tgaaaagtta tccattattg atgtcgtatt tcaggtatcc tgctaatcag gagaccagca
     2221 ctatctttaa cagccttatc tggatcggtg gagtatccga ttccggaaac cttcactttg
     2281 caggagaaag atatcgattt gaggggaccg atttccagcc cggaccggtt tccgatgtat
     2341 atgactcaac ttatgcaaga aagtactgga gattatggaa attaaacagg gaagatattg
     2401 aatatcatag gtataactgg tggaaatcag gttatcagcc tgttgatgct atcgtcacct
     2461 ggccgggcaa tggcatcacg gaagcaggcc aggctgatcg gctggcgcca ttcaaagata
     2521 tcggccagga tgggcggtat gatccgatgg aaggagatta cccgttgatc cgtggcgacc
     2581 aggcaattta ttttattaag aatgacgata agatccatac cgaatcggat ggtacaaggt
     2641 taaggttgga ggtccatgga atggtatacg gattcaatgc ccctggcgac agcgccctga
     2701 tgcataccgt gttcgttcat tacgatctga tcaacaggtc ggaaaacatt tattatgaaa
     2761 cttatgccgg tatctggacc gaaatagata tcggcggaat gtgggatgat tatatcggga
     2821 gcgatgtcgg tagaaatacg tattttggtt acaatggcga tgattttgat gaggatgata
     2881 ctgagcaacc ggaagagatc acgaaaggtt acgggaaaaa tcctccggca cagtcggtca
     2941 ccattcttgc cggccctgta atcgatgcag atggaacgga caatccaccc ggtggttgtg
     3001 attatggggt taacggcatt aatttcggca atgacatggt tgatgatgaa cggtatggat
     3061 tatgtagctt tatgaataac atacaactcc ctgctcatgt tggcaacccg tattatccag
     3121 ctcccatatt atataatatt cttaaaagcc agtggtatga cgggcaggat ctccttttcg
     3181 gcggagatgg gactatttac gattcgcggg cagtcgggcc cggatgcagg tatgttttcc
     3241 ctggatcatc tgatatcttg aatctcggta ccggatgttt acccccggag gagccatata
     3301 ataccggtgg tttttactgg accgacagca atatgaactc tcccggcgac cggcggggaa
     3361 taggctccat gggccctttc accttcaaac ccggcgatgt gcaggagatc gaactggcct
     3421 acgtcgtcgc caacggctgg aacgggccgg tatcgtcggt ggataagctg atggaatata
     3481 tcgatacgct gcgtgcccgg gtgcaaaacg gcggacttat tgtcccaaac agcgagctgg
     3541 gatacaatga accttccaaa atcccgggaa gcctgaaaat ttatccgaat ccggcatcgg
     3601 attttgttgt tgtcgaaata aagggaatag aatccggttg tgaatatgaa atctatgatc
     3661 tctttggcag gatggtgcta tcgggaatat tatcatttca agcacaaaac gtcatcaata
     3721 ccaatggatt atcatccggg ttatatgttt taatggtgaa aacggatgat atggtttatt
     3781 cggggaaaat cattagacgc tgatgcgact gtgatggaat tgaactaatt tcccccttcc
     3841 cccggatgcg ggaagggctg gggttggggg ctaagctaaa ccgtcaccac cgcgatccgc
     3901 ttcttcccgt ccatgctgac agtcagcggt ttatcgaagc ggatatggcg gaaatacttc
     3961 gtctggctga tcaccggctg ctgtttcagg atctcccagc ggatgtagct cttcgacagc
     4021 tcgggctgga tgctgaaata accgacgttc atcgacacaa cgttatggaa gaaatgagaa
     4081 cctgacgaag catccagcgg gtatccttcg aggctggtct cgatgatgac ccgggcatgc
     4141 gagatctgcg cccagttcac cgggatgccg atccagcggt cgcgcgtgcc ccaccggccg
     4201 ggaccgatca aaaggtattt cttgttctct gcggcaaact tccggttcat ctcctcgatc
     4261 tcataggtca tttgcatagt ctcacccttg tcgaaggtgt cgatatccac aaagatcaca
     4321 tcctcaatgt tatcgatcac cccgttgccc atactctttt cggcatataa aatgatcttc
     4381 tctttcggaa tcttgtcgat attgacatta tagtccatcg cattgccgat cagcggcttg
     4441 atctgcagca aataaaaggt ggcccgccca tgattgtctt tcgtcaggtc aacggcaaac
     4501 tcgatctcca ccggcgatcc catggcttcc ttgacgacat ccagcaccac ttcaatcgtc
     4561 tgagccaaag gaacaaagtt gtatttcagg atattggcaa aattgatcaa ccggggtccg
     4621 ggatgggtca agcccgggat aatgacctgg ttatccgcat cataaacgga ggcacagtgc
     4681 ttcaggttgc catgcatctc ggcttcgtaa atgtcgagtt tggccagccc ggcagtatcc
     4741 ccttccagga gatttatctc tttcttattg agatcgacgg cataaaactc cacctgagtg
     4801 ttctgcgact ggtctttggg cgataatatc tcggttgtag ggtatttggg cgaaaaacgg
     4861 taggctttct cgccgtcgac caccgatttg cccagcccga cggccgtcac ggcaaagccc
     4921 tcttcgggct gcatgtgggc ataagggtag aaattatacg actgcgccac accgctgatg
     4981 tgcgggtaga aatagttgcc gtattgcctc ccgaccacct cctggatcac taccgccatc
     5041 ttctcctctt cgatcttata gtgcaccgct tcgatataac cccgcgcttc gggcgaatag
     5101 atcgaggcaa aaacgagctt gacggcgtca agggcctgct tcagccgctc attcggatcg
     5161 ggatggcagt tgggaagcag ataggtctcg aaaatcccgg caaaaggctg catcagcgaa
     5221 tcttcgaaga ggcctgacga acgtacggca atcggtttgg tgatccgcgt cagcagtacc
     5281 ttcatccgct tcaccagcgt atcggtcagc tttgcatcca tgaaccgctt tttgatctcc
     5341 tcgtaatcgg tcgtcgaggc cacaaaatcc ctcagatggt tccggtccat gaaatactcg
     5401 aactcctctg tcccgataac cgaagtcttt ggcgcctgga tcttgatggt cgggatttgt
     5461 ttggaaaaat caaaattgta gatcagcgta ttgacaaaag ccagccctct tcccttcccg
     5521 ccaaggtaac catccatcag gcttacaatg ttggtctcgt cggtaatggc cgactcctcg
     5581 aacgggatga tcttaccctg gttctgttcg ttcctgaacc ggccgatcac atcgatgaga
     5641 taatccctta tctgacgggc attctcaaaa tcggtgacct tctgcgggtt caggatcttc
     5701 gccgcctgta tctctccgcg cgccgtgagc catagggaaa aatggtccct tttggcatga
     5761 tagagcaatg attcatcggg aattacccgc aacagcctct caaactcccg cagcgtccgg
     5821 gcttcggcga tcttagcccc ctgctcgtta cggtaaataa aattgccaaa tccgagaaag
     5881 tgggtgataa aactccggaa atcctggctc agcgtttcgg aatttttatt gatgaaggtg
     5941 catttcaagc gataggccat ctccgaattc tcctcttccg aactttggat gataatgggc
     6001 atatccttgt gtaccgaccg ggaatactcc accaggtcga agccggcccg ttcatccaaa
     6061 acgccattcc ggttgaattt cacatcggta atgaggcaca gaagataatc cctgaagcgc
     6121 tccatcacgg ctgtggcctc ctcgtaggtc gaaaccatca ggatcttggg cctggcgcga
     6181 agcctgagga ttttgtagag ttcgtcggtg cttacgtctt caatgatccg cttggtctgc
     6241 tccagcacaa tatgataaag catcggcaga taccgcgaat aatacttggc cgagtcttcg
     6301 accaggagga tgacacgcac catcccgatg tcggtatcgt tatcggcatt gatcttatcc
     6361 tccacatgct tgatcatggc aaaaaagacc gacgattccc cgttccagat aaaaaccttg
     6421 tcgatatgct ccagggcgtt tttatcttcg gagatcaaag ccatttcggc attgttgttc
     6481 agaagaagaa aaacggggat atagggatag agccgtttca ccttgcagct caggccgatc
     6541 ggggtacgtt tgtcggcccc catcatcatg atgatcagat cgaaatggcg ctggcccagg
     6601 atatccatcg cctcatcgat gccggaaacc ccggtgatac ggggaatcga tgtcaggttg
     6661 agggtatggt attctcccag gacatgttcc gaaaaccggc cttctttctc gatgctgtaa
     6721 gcgtcgtaaa gattggcgac cagcaggatc tctttcactt tgaagggcat caggtcatgg
     6781 taaatatccc ggtcttcgtt ggatttgttg ataaaccgct gcaatagctg ccggctggtc
     6841 actacaggct ggccatcgtc ggatttggcg gcagtacgtc cggccccggg ctttatgatg
     6901 gtctttgcca gctgcccgtt gataaagccg gtgatcatgt tggccaggtt ctcgatcagg
     6961 tgccgttcct cgatcatgaa cggcccttcg tcgatttcgg ggaactcctt caggtagtac
     7021 acctcgatat atcccacttt attgtcgatg gtctcgaaac gctgccgctg cacccaggga
     7081 gtcgctttga actcgatgct ggcaaactcc ttgccttcgt agagaatacg ggccgctgta
     7141 aattgcgggt actgccaggc gggagggaga atggtggaga tttgtttcag cgtctcctcg
     7201 atcgatttcc cctctttgat gatctgggtg gtgcggttga tggcggccag ctcttttaac
     7261 cgttcgatat tctgtgcctt cagctgaagg tagttggccg ggttgcccga agcgttttcg
     7321 ttcatgtgtc agcagtctga ataatgctct aaaattaaga aaaatcgggc ttttggagat
     7381 aaaaccgctt cctgttttaa acttttcatt ttgacaattg tttatcagat aaatttgcgg
     7441 acatgaggct gattattata ttattactga tacttactct gcctttcagc cctgccggcc
     7501 agacggcaga tgaagtcctt tcccgctatt ttgtcaccga acttgaaggc aatgtcttta
     7561 tgaggtggac cattacagca ggcaacacct gtgaggatac ttatattgaa cggtcggcag
     7621 acgggatcgc ctttgagcgg atcggcctga ttgcaggtat ttgcggcagc ccggacgcga
     7681 gcattacctt cgagttcacc gatacggtcc cggtctacaa tcaaatcagc tattaccgcc
     7741 tgatgctggg atattttggc tatacaaaac cgctggccat tgaggtaaaa aagttcaatg
     7801 atgaaggttt ttttgcgggt cctaatccct tccgcgattt tacacgtatt gcctttagaa
     7861 atgacgataa tgaggaatac cggctcctgg tctgtgatat gcagggacgc tctgtcctgc
     7921 aaatgttgac caatggaaat gaatttatca tccggaggga agagctggga tcagggatat
     7981 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatggaatc caggccgaaa
     8101 ataaagatcg ggcaaactac caccgaccga tttgtagaaa tcgccggctg ggctgcgctg
     8161 gcttttatgt ggatcattac actttactat ttccggaaac ttcccgaaac catcccgact
     8221 catttcaata tgaaaggtac tgcggacgga ttcggacaaa aatcgacgat ctttctgctt
     8281 ccagccatcg gaaccgtcct gttcatcggc atgacaattc tgaaccgctt tccgcatatc
     8341 ttcaattacc cggtcaggat caattcagag aatgcgatca gacaatatac cctggcaacc
     8401 cggctgttgc ggatatttaa ggtatcgatc gtgctgatct ttttaatgat aacctggatg
     8461 acttccgctg cggccaacaa tcagggtaaa aactcttttg cctggtttct gccggtgatg
     8521 ctggcaatta cctttgttcc gctggggatt tatattttta aatccttcag ggctaaataa
     8581 ccgctgaaaa gttttagcca cttccggatt taaaatttga gtccactccg aaaagtaatc
     8641 tatattccaa agtacccacc cctgcccctc cctacgagta gggaggggtt aggggagggt
     8701 aaattcactt ttcggagctg actcaacttt gaactttgaa ctaaatcagt tttctcttaa
     8761 aacgaactcg tcgtattcca gttcaaaccg cagtttgtgt ttcgattctt ccatggccag
     8821 cgacaggaaa atatctttca gttcctggtt cggggccctt tcggccaggt tcgagtagag
     8881 cctgaaagcg gctttctccc gtttcatggc cagcaccagt gcctgctcat agctgagggc
     8941 accactgaca ttaacctcca ccgtgtaatc ggcgatcttc aggtcggcca ctttctctat
     9001 gggcatggtg aacagtccct gctcgctgat ctgcgtcagc cgtttcttgt gcctgatctc
     9061 ttccccggca aattccagga acactttctt catctcttcg gaatgggctt ccttagccag
     9121 cccggtgtag aaatcaacgg ctttttgctc ctgttctatg gcaaaagcca ggattgcttc
     9181 aatattttca aactctttca taaacttctg ttttaacatt ttcaaatgcc tctgtaaact
     9241 cagaatcaaa tcgtttcacc aggtaatcgc aaccgtgttc gaataatctg gcttgattat
     9301 cctggttcgt taccgtgaac aaatggaaag attcttttcc cggttccggc agcagtgaga
     9361 caatttcatt caaaacatca tgatagtcag cactttgatc aattccttcc gccttcagaa
     9421 gatcactcaa ctgctccagg gtagtcctgg agaccggggt gaaatgcgcc tcaaattcct
     9481 ggatcacctt ctgcgtgttg gtgaagcttc cgcccgattc aacggggaaa gaggccggga
     9541 ggatcaaatc ggccagcgcg gttgtttcgg tcatgaagta atcctgaacg acaatgaacc
     9601 cggcctgttt cagccagccg tgaattttag ctttatcgaa ggtacaaccg gccggatctt
     9661 cgccgaaaat aaagaggttc ttcaacgttc cgctctccag tctttcaacg atcggaacag
     9721 gatttatcgc cgggaagtct gccatcttcc atgctttctg aatcctggac ctcgactctg
     9781 catcatgcag cgacactccg ccggcgccat agttgcggca aatccccata tcatgcaatc
     9841 cctggctgtt gttcttctcc ttcaaggcaa tcagcccgct gccggttttg cccagcttcc
     9901 cggtgatcag cgacaggttt tgcagctccg tgcaggcatt cgacgacaat tccttttcgg
     9961 aaaagacgat gaccgcccgt tgttcttcgt tgtaacgggt ggcaaattcc acgatttctt
    10021 cggcagaaac accggatctt tgaaccagat ccgtaaaact ttctttcaga agttcgtcct
    10081 tataatcatc aaaaccttta caattatccc tgataaagag gttattatga agattgttgt
    10141 gaacaagata aaaattgatg gctttgataa aatcataata cgatttaata acaagcaccg
    10201 aatcggcttt atgagcctca gcgctattgt cttttgttgt aaccagttca accttaacgc
    10261 ctttgatgta ttgagcattg tagatattaa aaccggccac cgcgttatca cggctgatct
    10321 ctgatccgat gaggaaaacc ctgccggcat cgcggatctg gctgaacggc gttgccgccg
    10381 tggtgttgtt ccgatagccc gccccgcggc ccaggtagtg aaaactggtc acgttattgg
    10441 tcttaacgcc ggcccgggcc agtttctgga ccaggtagag ctcttcgttc gacaaccggg
    10501 cgccggcaaa gaaagcattg tcgtcggctc ctgtggaatg gatcttctga cggatgactt
    10561 cgtaagcttt ttcgaaggtc acctcttcga atttcccgtt caccttaagc aacggtgagg
    10621 taattctcga ccggtcgttc agatattggt aaccgaacct gggataccgg caaaggttgc
    10681 cgtcgctgtt cacctggcct tcaacgcctg aaacgtgcat aacgaaacgg cttttgtggt
    10741 cgagcgatat cgaacagccc accgagcaat agttgcagat ggtctctgcc ggctggagcc
    10801 ggaccggacc cggtttgaac ggcacattct ctgtaatggc gccggttggg caggtagaaa
    10861 tgcacatccc gcacgattcg caggtggttt catacaaagc attgcccatc gacggggcca
    10921 cataagtgtc gaaaccgcgg tttaccaggc ctagcgcccc ggcgcctact acctcgcggc
    10981 agatccggat gcagcggctg cagaggatgc atttgttgtt atcgatctca atatagggat
    11041 gccggaaatc gacccggtac tccttaaact cacccgcctg tgctttctgg tcggcattat
    11101 attcggtaga gaatttcttc aggtcgcagg taaaaagggc ggtacagccg cattccaggc
    11161 atcgttgggt ctcccggaaa gccacctcct ccgactcata gcccagttca acctctttga
    11221 agttgagccg gtctttcgga tcgagtgtcg gcatctcatg ccttttcctc tctgtgaata
    11281 atccgtgata atctgtgtta ctctgtggtt taaaaacatc cttagaagaa tagaactcct
    11341 tcgttatagg caccaccggc agccctttca ggtaaagatc gcagctatgc gatgctttcc
    11401 tggcctgcgc gatcgcctgg atcagggtag ccggccccgt aacgccatcg ccggcggcaa
    11461 aaaccgacgg gatccctgtc tgcaaggtat ccggattggc ttccaggtcg ccccagcggt
    11521 tggctttcac ctccccgtcg gtcgcatatg acttaatgtc gtcaaggaaa tcggcctcgg
    11581 ttttctggcc gatagcagca aggatataat cgatctccac atcgaactcc gatcccggca
    11641 ccggcaccgg ccttctcctt cccgacgcat ccggttcccc cagctccatt ttcacgcaag
    11701 taatactctt cacgcctccc gcttcactct tcacgacttt cacgggaagt gtcagaaaca
    11761 tatactccac cccttcgagc tttgattcat ggatttcgat cgggttggcc ggcatctctt
    11821 tttcggtgcg ccggtagatc acgtatacct tgtcggcgcc acaacggatg gaagtacggc
    11881 agcagtccat cgccgtattg ccgccgccga tcaccgcaac cttcttacct ctaaagttaa
    11941 agcgctggcc tgtcatttcc atctctttaa ggaaatcgat accggaaaag acgccctccg
    12001 catcttcccc ttcacatccg atcagggtcc ctttctgcga accgatcgtc aggatcatcg
    12061 catcatactg gtttttaatg tctttatatg aaatatcgtt cccgaatttt ttattataga
    12121 agatattgac ccccatttcg gtgatattgt cgacctcttt ctggaggagg tcgttaggga
    12181 ggcggtattc ggggatgccg tagcggagcc aacctccggc cctgggactg gcttcgaata
    12241 tatccacctg gtgcccttct ttttgcagga agaaaccgca ggagagccct gccgggccgg
    12301 cgccgatgac ggcgactttc tttcctgatg cgggctttat ttccggtctc catttatccg
    12361 gtgaatcgat atcgatatct gaggcgaacc gtttcaggta gtcgattccc acggcggccc
    12421 cttcgtcgag caggttgcgg cggcaggcca cttcgcacgg acggacgcag acgcggccgc
    12481 agatggccgg cagggggttg gtctctttga tcagtgcgac ggcatcgtgg tacttgcctt
    12541 tctcgatcag ggagatatag ccctggacat ccacgccggc aggacaagtc tgcttgcacg
    12601 ggccgataca gtcggcgtag tggttgctca ccagcaggtc gagcgctgtt ttacgggctt
    12661 tatgtatctt ttcattatcc gtagtgatct tcataccctc atacagccgt gtcgaacatg
    12721 atggttgcag gcctttcatg ccctcgatct caaccacgca gacgaaacag gaggtaaaag
    12781 gctcgaggcg cgggtcgttg caaagggtgg gtatttcgat cccgttgcgc ttggccagct
    12841 ccaggatggt ctctccgcgg gaaccctcga caatattgcc gttcaggatg atattgactt
    12901 tttcgctcat agttttgctt ttcctttctg atatttctat ttaaccacga aggcgctcaa
    12961 aggactttca ctaaggcgca cgaagtactg aggtgctttg acatctacct ttgtgttcct
    13021 ttgtgaaatc cctttgtgtg cctttgtggt tataatattc atgcaatatg ggtgttatga
    13081 taattttatc gcttcgaatt tacattttgt ataacacatg ccgcatttga tacagtcttc
    13141 ctggcggata tagtggaccg tcttgcgctc gccgtcgatg gcatttaccg ggcagttctt
    13201 cgcgcagacc gtacagcctg tgcatttctc tccgatcacc tcgtaggtca ggagtgattt
    13261 gcatgccttt gccgggcatt ttttatcgcg gatatgcgcc tcatactcgt ggcggaagta
    13321 acggatcgtg gtcagcactg ggttaggcgc cgtctggccg agtccgcaaa gtgagttgtc
    13381 cttgatctgg taagcaagtt cctcgagcct ctcgatgtca ccctcctccc cttttccttc
    13441 ggagatacgc tgcaggattt ccagcatacg tttcgttccg acgcggcaga aggtgcattt
    13501 accgcagctc tctttctggg tgaagtcgag aaagaattta gccacatcga tcatgcaggt
    13561 atcttcgtcc atgaccacca tgccgccaga gcccataatg gcgcccgtag cgttgaccga
    13621 gtcgtagtcg acgatcgtat cggccaggta gtccgggata catcccccgg aagggcctcc
    13681 catctggacg gccttgaatt tacggtcttt ctggatacct ccgccgagtt tgaagatgat
    13741 gtcgcggatc gtgatgccca tcggtacttc caccagcccg gagcgcttga tcttgccggc
    13801 cagggcgaag actttcgtcc ccttgctttt ctccgtgccg tattttgcgt aggcataggg
    13861 cccgtttaag atgatccacg ggatgttggc ataggtctcc acgttgttga tattggtcgg
    13921 cttgcgccag aggcccgata cggcaggaaa gggcgggcgt ttgcgcggca ttccgcgctc
    13981 tccttcgacc gaagcgatga gggccgtttc ttcgccgcat acgaaggcgc cggcgccctc
    14041 tttgatgtaa atatcgaaga gggcgccggc gccttcgtat gcggcgaaga aacggccctc
    14101 atc
//
LOCUS       DAOPNH010000047        13937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4433_length_13937_cov_5.665826, whole genome shotgun sequence.
ACCESSION   DAOPNH010000047 DAOPNH010000000
VERSION     DAOPNH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_13937_cov_5.665826"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKZ74_03915"
     CDS             <1..319
                     /locus_tag="PKZ74_03915"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPM91934.1"
                     /translation="DPDETMVMQARETGTDRVELYTESYAREFRKDREKAVRPFVMAA
                     MAAQQAGLGINAGHDLNLENLRFFKERVPGLLEVSIGHALICDALYYGLENTIQLYLR
                     QLK"
     gene            329..1102
                     /locus_tag="PKZ74_03920"
     CDS             329..1102
                     /locus_tag="PKZ74_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPM91935.1"
                     /translation="MDLFFREYGQGQPVVILHGLLGISDNWVAFSRRLAGEGFRVLIP
                     DQRNHGHSPHHPVLNYYALTDDLSEFIARHQLENPVLIGHSMGGKVAMRYTLENPETV
                     KKLVVVDTSLRAYYRFNYHLRLIDAMLAVDFDKIVTRQEVEKQLYNKIGDSRLVMFLM
                     KNLFWKEKGKLGWRHALAAISQNIDAMYDGVFYSVKYSRPALFIRGGKSDYILDEDIP
                     AITQSFPQSIIKTIENGTHWVHADEPELFYRYVLGFMKS"
     gene            complement(1106..1951)
                     /gene="tatC"
                     /locus_tag="PKZ74_03925"
     CDS             complement(1106..1951)
                     /gene="tatC"
                     /locus_tag="PKZ74_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPM91936.1"
                     /translation="MPEEISNKKDKGKAAEKEMSFWEHLEELRWHLLRSAVVLVVLAI
                     GAFLMRRFIFDVIILAPKEPGFISYRALCTIGKWLHTDSLCLDIEPLQIVNIQMSGQF
                     MVHLYISLMTAVVVALPYLLWEIWSFVRPALLPKEKKYSKGIVFYSTLLFVLGVLFSY
                     FLIVPLTVNFFATYSVSDSVNNTITLNSYISTIVSLTLSTGLVFELPIVVFFLTKVGI
                     LTPAFMRKNRKYVIVILLVLAAVITPPDVFSQIMVTIPMLLLYEMSIVISARVLKKRK
                     EELAG"
     gene            complement(1954..2592)
                     /locus_tag="PKZ74_03930"
     CDS             complement(1954..2592)
                     /locus_tag="PKZ74_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPM91937.1"
                     /translation="MKELNRTDRLTIATTMLAIILIIGLVTMRKPAVEFAIPATEVAK
                     LATDPSYVIPVTILAEMMKNNDPALALIDVRNPVKFRQEHIPSALNIPSADLLSEQNL
                     NLIKNLAEEDKKIIIYGGNHLEANGSWLILKQLGINNLYILDAGMEGYNLYRQNLSVN
                     LPFEDVKSERPAYNYNEILNSFGTSSSAQETGKTEPVRITKKAKKSAAEGGC"
     gene            complement(2612..3226)
                     /locus_tag="PKZ74_03935"
     CDS             complement(2612..3226)
                     /locus_tag="PKZ74_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPM91938.1"
                     /translation="MSRKYIIPVALLLILSIVLVLLPRKQDHSQMSPEELLRQINSTS
                     RFVSTDEVADRLIRKDPSLALIDVRNDKLFSEYSLPGALNIPVDNILADESQEILSQE
                     GLEFVFYSTDDILADQTWIILKRKGLKNIYVLKGGLNEWFRTFFTNPPPAETADSEEI
                     ALYQFRMGVRQYFTGTPADSPEKSSAEKIIITPKKKKSAAEGGC"
     gene            complement(3238..3828)
                     /locus_tag="PKZ74_03940"
     CDS             complement(3238..3828)
                     /locus_tag="PKZ74_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPM91939.1"
                     /translation="MGPLIPNDIIPADWNNAIALLLGMAFGFVLEASGFSSSRRIMGT
                     FFGYDFVVLRVFFTAAVTSMIGILYLDYFGWVDFGQLYIHPTYLMSAIIGGVIMGFGF
                     ALGGYCPGTSYCAVAIGKLDALAYSIGLFIGIYLFTFAYPLLEGLYNGSFMGYVTAPE
                     VMGINQYLFVFFVVIAALGMFFVATIVQRKVKKVEY"
     gene            complement(3840..4373)
                     /locus_tag="PKZ74_03945"
     CDS             complement(3840..4373)
                     /locus_tag="PKZ74_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPM91940.1"
                     /translation="MMENNRKDRSQNYLNPYFGGVLLGIVLILTFYITGRGLGASGAA
                     KSTVVTTIQSVAPEYAKKSHYVSQFISDDNHPMNNWLVFEVLGVFAGAFLSGTLYGRL
                     KPRVEHSPKITSKKRLVVALLGGLLFGFGSQVARGCTSGAALSGMAVLSFGGIITMMG
                     IFGSAYLFSWIFKRLWI"
     gene            complement(4392..5333)
                     /gene="nrfD"
                     /locus_tag="PKZ74_03950"
     CDS             complement(4392..5333)
                     /gene="nrfD"
                     /locus_tag="PKZ74_03950"
                     /inference="COORDINATES: protein motif:HMM:NF025940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPM91941.1"
                     /translation="MKEELFVSGRDIPNIDPFLNIWHWQIPIYLYLGGLAAGILFFAG
                     IFTILNKEKEMPATVKWAPMLVPGALVIGLLMLFLDLKHQLFFWRLYTTVRFESPMSW
                     GAWVLMAVTPLSFIWAATYIKEIRPEWNWKFNLLEKFEAFAVKNRKPMAWIFIVLSVV
                     LGIYTGILLSAFNARPLWNNSILGPLFLVSGLSTGTAVIMWITKNPRERIIMSRIDLV
                     LIIVELFFITHMMMGMLAGSSVQIAAAGLFLGGQFTVSFWVFVIVLGLVFPAVLEILE
                     LAGFKIPVAIPALLILFGGLVFRFIMVEAGQITRYLY"
     gene            complement(5351..5884)
                     /locus_tag="PKZ74_03955"
     CDS             complement(5351..5884)
                     /locus_tag="PKZ74_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM91942.1"
                     /translation="MRYAMAIDTRKCVGCSDCVVACQLENNVPVGFCRDWVTESVHGS
                     YPNVELEIRSERCNHCENAPCVRCCPTGASHIVEGGIVLVTGNKCIGCGACIQSCPYD
                     ARYSHPEGYVDKCTFCHHMVSKGGDPACVAVCPTKCMYFGDLDDPNSKVSNALKNRKF
                     KVLAPEAGTNPQIYYLI"
     gene            complement(5881..8127)
                     /locus_tag="PKZ74_03960"
     CDS             complement(5881..8127)
                     /locus_tag="PKZ74_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPM91943.1"
                     /translation="MKSRRDFIKISALGIGGAAVAGSAIKWVWGNKNENNESAATSDL
                     PSLTRYPTYCEICFWKCAGWVYLNEEGKIQKIIGNEQDPHCNGRLCPRGTGGPGMYHD
                     PDRLKTPLIRAEENGRQFFREASWDEALNLVADRMKQIAAKHGPAGMALFNHGSSGTY
                     FGHVLKAYGSGNITAPSYAQCRGPREVGFELTFGGGLGSPEPTDIRDTRCLVLIGSHL
                     GENMHNGQVQEMSDAIDRGATIITVDPRFSTAASKSKYWLPIKPATDMALLLAWINVI
                     ITENLYDRDYVEKYTMGLEQLRAHVEKMTPEWAYGITTIKPHIIRETARAMANASPAV
                     IVHPGRHVTWYGDDTQRSRAIAILNALLGSWGRRGGFYFKESLSIPDFPHPEYPKPAW
                     SWKDTLDGKYPLARLSVANAIVDASIPAEGKEHTIRGWIVSGTNLPMTMPQKDQLLKA
                     MQALDLLVVVDTMPMEITGYADVVLPECTYLERYDELRDSPAREPFLGFRMPAATPMY
                     ESKPAWWIAKQLGERLGLGAYFNYTDYKEVLAWQLNQLGTSIEEMERMGVKKFERSSG
                     SLYMEEGQPMEFNTNSGRIELYSTELASLGFDPMPVYTAHPEPPPGFYRLIYGRAPMH
                     TFSRTANNPNLVDLMKENSVWIHPDVADIWGLKKDQYIWLKNQDGVTSHFPIKVRITE
                     RIRWDSVYMVHGFGHQDKKLSLAYGRGANDSDLITEVKVDPLMGGTGMRGNFVTILTD
                     KPGKETES"
     gene            8330..8740
                     /locus_tag="PKZ74_03965"
     CDS             8330..8740
                     /locus_tag="PKZ74_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPM91944.1"
                     /translation="MMRIREADGKGRRRFILTLGTLMLIPLAGIWNAMAKRAQKREGT
                     VKSVVPIEDISGDVFYAEEFVIIRESGEFTVYSTRCTHLGCRLEPAGDGLLRCPCHGS
                     EFDLKDGNIRKGPALNPLKTLIFTIHENNLIIYT"
     gene            8744..11479
                     /locus_tag="PKZ74_03970"
     CDS             8744..11479
                     /locus_tag="PKZ74_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HPM91945.1"
                     /translation="MIRFFDIVQKYLNDTVGGLALSSLIICILSGIFVSLPYDAASPF
                     NSLSLIMIDNPSAVYFRNVHYWSAQLFLALTLLHITEHLLRKSEKLVTRAVWWRLTLS
                     VPVIFFVMISGFILKGDADSVSAFRVLSALFREIPWVGSLLSFSITGNEPSFLTLFMH
                     HIATATIIIVAVIAEHYRKFWPPAGSFFLLTFFILIISYFLHPFFGVETGKGPWYFVG
                     LQEFLRYFSRPALIWVLVAVFLAGLGIVSATGPVQNRRIKISIAVFFLFYGLLTVTGL
                     FFRDFDQSLRFPWNIHDKGWMGVAIRPLALSVPEDHDFSGPVPLVNGHYEGCLVCHDN
                     VKGLSVAHDPAAVGCSSCHGGNPATLDKHLAHRNMILVPGQLADARRSCGTASCHPGI
                     PDRIEGSLMTTMSGVISVNRYVFGEAATPDIRAHVKDVGNSAADRHLRDLCINCHLGN
                     PKTYTGPNNSISRGGGCNACHLHYSDSALVSLANYRQSATIEDSISFYHPQLNLKIPD
                     DNCFSCHSRSGRIALSYAGWHETLYNDNDIPDTGNFKVLDDRRVVQFVGQDVHHKAGM
                     SCIDCHLSRELMGDGESYLHKEEQVKIGCSDCHFMDNPLTLFAKDADPESVKTLTLRN
                     WIDNSLRLLKTATGGYPIINSRVSEDGGAWLALKSGDSLLALRSPAAACTRGKAHRSL
                     SCGSCHSSWVPQCIGCHNSFDPDAPGYDMLENRERTGSWVEYAGLFLTGKPALGIIED
                     KRGNRTVTTFTPGMVISIDTGSYDQNQDGGRIFRRLYAPVSAHTTVRQGRPCRSCHLD
                     PLAIGYGRGELIYSMDRDRGTWDFVNRIERSPYDNLPEDAWIGFLEEPDAAVSTRSNA
                     RPFNLEEQKSILTAGACLECHEENSAVMQAALDNFDSVMIKVSKRCVLPVWPAAN"
     gene            complement(11476..12039)
                     /locus_tag="PKZ74_03975"
     CDS             complement(11476..12039)
                     /locus_tag="PKZ74_03975"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPM91946.1"
                     /translation="MMTGIILAGGRSSRMGHDKGLISFHGKLLVQYSIDVFEKLCREI
                     LISSNNLEYEQFGYPVIPDVTGEIGPMGGLYSGLLKSTNEINLVLSCDMPFVTGNIFQ
                     MLIHRAGTAQVCIPYYGQEKYEPLCGLYRKNTLETMTRSIFEEKNYKLPDLFSRVETS
                     LLLITEIDPPLPEIYFKSINSPEDLAI"
     gene            complement(12036..13289)
                     /locus_tag="PKZ74_03980"
     CDS             complement(12036..13289)
                     /locus_tag="PKZ74_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPM91947.1"
                     /translation="MAINLHPRNLRIEDYDYPLPDDRIALFPLEQRDSSKLLLVRNGR
                     TGEDIFSNMATHLPPDSLVVFNETRVIHARLLFRRPTGSRIELFCLEPVAPYRDHLRA
                     FGCTGQADWLCLVGNSKRWKHGGLELTAGEGKDQFVLKAETTSRNEDGSSVIRFTWHP
                     EHLTFSHILEQAGKIPLPPYIHRDPVHNDERTYQTIYASREGSVAAPTAGLHFSPEVL
                     ASLEAKGIKSQRLVLHVGAGTFRPVSSERLEGHEMHAEQVDLPLLALKDLRGNLDRKI
                     TAVGTTTTRLLESLYWHGVKVIMGLSEPSVLDVRQWDPYDLPVPGKISREESLDTVIS
                     GCERMGAGSVRGATSLIIAPGYRFRYPDILITNFHQPRSTLLLLISSFIGKNWKEAYR
                     YALENNFRFLSYGDSCLFYKSEPGR"
     gene            13390..>13937
                     /gene="pssA"
                     /locus_tag="PKZ74_03985"
     CDS             13390..>13937
                     /gene="pssA"
                     /locus_tag="PKZ74_03985"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPM91948.1"
                     /translation="MNFKKNIPNGITLLNLVSGGVSVIMALSGEPACAAWLILLAAVF
                     DFLDGLAARLLNARSAIGGELDSLADVISFGLAPSAILYALMLKSPNLPEFAAGEYQL
                     FPFSALLLAAFAAARLAKFNTDPGQAIEFRGLPTPATGLFVAAFPLMVHYQGDMEYVR
                     LLTGNFYLLLAVVIVLSALMVSS"
BASE COUNT         3324 a         3453 c         3630 g         3530 t
ORIGIN      
        1 cgatccggat gaaacaatgg tgatgcaagc ccgggagacc ggcaccgacc gggtcgagct
       61 ctatacggaa agctatgccc gggagttccg aaaggatcgc gaaaaggcgg tcaggccttt
      121 tgttatggcc gcaatggcag cgcagcaggc ggggctgggt atcaatgccg ggcatgacct
      181 gaacctggaa aacctccggt ttttcaagga acgggtgccg ggactgctgg aggtctctat
      241 cggtcatgcc ctgatctgcg atgcgttata ttatgggttg gagaacacga ttcagctata
      301 tctcaggcag ttaaaataag cgtagcagat ggatctcttt ttcagggaat acggccaggg
      361 ccagcccgtt gtgatcctgc acggattgct cggcatttcc gataattggg tagccttttc
      421 acgcaggctg gccggcgaag ggttccgggt gctcattccg gatcagcgca atcatggcca
      481 ttctccccat cacccggtgc tgaattatta tgccctgaca gatgacttgt cggagttcat
      541 tgccaggcat cagcttgaaa atcccgtact catcggccat tctatgggag gaaaggtagc
      601 catgaggtat acactggaga atccggaaac ggttaaaaag ctcgtggttg tggataccag
      661 tctccgggca tattaccggt tcaattatca ccttcgcctg atcgacgcga tgctggccgt
      721 ggattttgac aagattgtga cgcggcagga ggtggaaaaa caattgtata ataagatcgg
      781 tgacagccgg ctggtgatgt ttctcatgaa aaaccttttc tggaaagaaa aagggaaact
      841 tggctggcgt cacgcactgg ccgcgatcag ccagaacatc gacgccatgt acgacggggt
      901 gttttactcg gtgaaatatt cccgtcctgc tttatttatc cgcggaggta aatcggatta
      961 tatcctggat gaagatattc ctgccatcac acaaagtttt ccccaatcca tcattaaaac
     1021 aatagagaac ggtactcact gggttcatgc cgatgagccg gaacttttct accggtacgt
     1081 tctcggattc atgaaatcct gacctttaac cggccagctc ttctttacgt tttttcagca
     1141 cccttgccga aatgacaatg ctcatttcgt agagcagcaa catcgggata gtgaccatga
     1201 tctggctgaa cacgtcggga ggtgtaataa ccgctgccag aaccagcagg atgacaatga
     1261 cgtatttgcg gttctttcgc atgaaagccg gggtcagaat accgacttta gtaaggaaga
     1321 agaccacgat cgggagttca aagacgagcc cggtggagag ggttagcgag acgatggtgc
     1381 tgatgtaaga attcagtgta atggtattgt ttaccgagtc gcttacgctg tatgtggcaa
     1441 aaaagttgac ggtgagcggt acgatcagga aatagctgaa gagtaccccc agaacgaaca
     1501 ggagggtgct gtaaaatacg attcctttag aatacttttt ttcttttggc agcaaggcgg
     1561 gcctgacgaa cgaccatatt tcccagagca gatagggcaa agccacgaca acagccgtca
     1621 tcagcgagat ataaagatgg accatgaact ggcccgacat ctggatattg acaatctgaa
     1681 ggggttcgat atcgaggcag agggaatcgg tatgcagcca ttttccgatc gtgcacaagg
     1741 ccctgtaaga aatgaaaccg ggttcttttg gcgccaggat gatcacatcg aaaataaacc
     1801 ggcgcatcag gaaagcgccg attgccagaa ccaccaggac tacagccgaa cggagcaggt
     1861 gccaccggag ttcctccagg tgttcccaga agctcatctc tttttcagct gcttttcctt
     1921 tgtctttttt attggatatc tcttccggca tttttagcac ccgccttcgg cagcagactt
     1981 tttggctttt ttagtgatcc tgaccggttc ggtcttgccg gtttcctgtg cagaagacga
     2041 agtcccaaaa ctattcaaaa tttcgttgta attgtaagcc ggtctttcag atttcacatc
     2101 ttcaaaaggg agattaaccg aaagattttg cctgtatagg ttgtaacctt ccattccggc
     2161 atccaggata taaagattgt tgatgcccaa ttgcttcaat atcagccagg aaccatttgc
     2221 ctccaggtga ttgccgccgt agatgatgat ttttttatcc tcttcagcca ggttctttat
     2281 caaattcagg ttctgttccg agagcagatc ggcagaaggg atgttcaaag ctgaagggat
     2341 gtgttcctgc ctgaatttta cgggattcct gacatcgatc agtgccaggg ccggatcatt
     2401 gtttttcatc atttcggcca gtatcgtcac tgggatgaca tacgacgggt cggtagccag
     2461 cttcgcgacc tctgtggcag gaatggcaaa ttcgacagcc ggttttctca tggtcaccag
     2521 cccgatgatg agtatgatgg ccagcatggt ggtggcgatg gtcagccggt cggttctgtt
     2581 caattctttc atggcattct attaaagagt ttcagcaacc gccctcggcg gccgattttt
     2641 tctttttcgg ggtaatgatg atcttttcag cgctgctctt ttcggggcta tccgcagggg
     2701 tgcccgtgaa atactgccgc acgcccatcc tgaactggta cagggcgatc tcttccgagt
     2761 cggcagtttc agccggggga ggattggtga aaaaggtgcg gaaccattca ttcagcccgc
     2821 ctttcagtac ataaatattt tttaatccct tgcgtttcag gatgatccag gtctggtcgg
     2881 ccaggatgtc atcagttgaa tagaaaacaa actccagtcc ttcctgggat aagatttctt
     2941 ggctctcatc tgccaggatg ttatcaaccg gtatattcag agcgcccggc agcgagtatt
     3001 cgctgaacag cttatcgttc ctgacatcaa tgagggccag tgaaggatct ttgcggatca
     3061 gccggtcggc cacttcgtcg gtgcttacga agcgcgaggt gctgttaatt tgtcgcagca
     3121 attcttccgg gctcatttgg ctatgatctt gtttgcgcgg aagaagaacg agaacgatgc
     3181 tcaggatcag gagcaatgca acgggtatga tatattttct ggacatagga tatcggatta
     3241 atattccact tttttgactt tccgttgtac gatggtggct acgaaaaaca ttccgagggc
     3301 tgcgataacg acgaaaaata caaacaggta ctgattgatc cccatgacct ccggtgcagt
     3361 aacatacccc ataaaacttc cgttgtacaa tccttcaagc agcggataag caaaggtaaa
     3421 gagatagatc ccgataaaca gcccgatgga gtaagccagg gcatcgagtt ttccgatggc
     3481 gacggcgcag tagcttgtcc cggggcaata tcctcccagg gcaaacccga atcccatgat
     3541 cacaccgccg atgatcgccg acataagata cgtaggatgg atgtaaagct ggccgaaatc
     3601 gacccacccg aaataatcga ggtagagaat accgatcatg gaggtgacgg ctgcggtaaa
     3661 gaaaaccctg aggactacaa aatcgtatcc gaaaaatgtc cccatgatcc ttcgggagga
     3721 ggagaagccg gaggcttcga gcacgaagcc gaaggccata cctaataaca gggcgatggc
     3781 attgttccag tcggccggta taatatcgtt tggtataaga ggtcccattg ttttacaaat
     3841 taaatccaga gtcttttaaa tatccagcta aaaaggtaag ctgagccaaa aatgcccatc
     3901 atggtaataa ttcccccgaa cgagagtacg gccatccccg acagggctgc gccgctggta
     3961 catcccctgg ctacctgtga gccgaaacca aacaggaggc ctccgagaag cgccaccaca
     4021 aggcgttttt tcgaggtgat tttcggggaa tgttccaccc tgggtttcag gcggccgtaa
     4081 agcgttcccg acaggaaagc gccggcgaac acccccagta cctcgaacac cagccagttg
     4141 ttcatgggat ggttatcatc gcttataaac tgtgaaacat agtgtgactt ttttgcatat
     4201 tcgggagcga ccgactgaat tgtggtcact accgtgcttt tagcggcgcc gctggcgcct
     4261 aatcctcttc cggttatata aaaagtgagg atgaggacaa tgccgagaag taccccgccg
     4321 aaatagggat tgaggtagtt ctggctccgg tcctttctgt tattttccat catataataa
     4381 atatcttaag attaataaag ataacgggta atttgtccgg cctccaccat aataaaccgg
     4441 aagaccaggc cgccaaaaag aatcagcaag gctggaatag ctaccgggat tttgaagccc
     4501 gccagttcaa ggatttccag taccgccggg aagaccaatc cgaggacgat cacaaacacc
     4561 cagaaggata cggtgaactg cccgccaagg aacaggccgg ccgcggcaat ctgaaccgat
     4621 gacccggcca gcatccccat catcatgtgt gtgataaaga aaagttccac gatgataagg
     4681 accaggtcga tccggctcat aatgatgcgt tcccggggat tttttgtgat ccacatgatg
     4741 acggcggtac cggtggaaag gccggagacc aggaaaagcg gccccaggat ggaattattc
     4801 cagagcgggc gtgcattaaa ggcagagagc aggatcccgg tataaatacc cagaactact
     4861 gacaggacga tgaaaatcca tgccatcggt ttccggtttt taacagcgaa tgcttcaaat
     4921 ttctccagga gattgaactt ccagttccac tcgggcctga tctctttaat gtatgttgcc
     4981 gcccagatga acgacagcgg ggtgacggcc atcagcaccc aggcacccca cgacatcggg
     5041 gattcaaaac ggacggtcgt gtaaagtctc cagaaaaaga gctggtgctt caggtcaagg
     5101 aacagcatca gcaggccgat gactaatgcg ccgggaacca gcattggtgc ccactttacc
     5161 gttgccggca tctctttttc cttgttcagg atggtaaaaa ttcccgcaaa gaaaagaatg
     5221 ccggccgcaa gcccacccag gtaaaggtat atggggatct gccagtgcca gatattcaga
     5281 aaagggtcga tattgggaat atcgcgaccg ctgacgaata attcttcttt catatccagt
     5341 tataaagaga ttaaattaga taataaatct gcggattggt cccggcttcc ggggcaagta
     5401 ctttgaattt cctgtttttt aatgcgttgg aaaccttact gttagggtca tccaggtcgc
     5461 cgaagtacat acatttggtc gggcagactg ctacacaggc cggatcacct ccctttgaaa
     5521 ccatgtggtg gcagaaggtg catttatcca catagccttc cgggtgcgaa tagcgggcat
     5581 cgtacggaca cgactggatg caggccccgc agccgatgca tttgttcccg gtcaccagca
     5641 cgattccccc ttccacaata tggctggcgc ctgtcgggca gcacctcacg caaggggcat
     5701 tttcacaatg gttgcacctt tctgaacgga tctccagctc gacattcgga taacttccgt
     5761 ggaccgattc ggtcacccaa tcccggcaaa agccaaccgg cacattattt tccagctggc
     5821 atgcaacgac acagtcgctg cagcctacgc atttccgggt atcgatagcc attgcatatc
     5881 tcatgattca gtctcctttc ccggtttatc cgttaagatc gttacaaaat tccctctcat
     5941 gcctgtgcct cccattaacg gatccacctt cacctcggta atcaggtcgg aatcgtttgc
     6001 cccacgtccg taagcaaggc ttagtttttt atcctggtgt ccgaaaccat gtaccatata
     6061 aaccgaatcc caccggatcc tttcggttat cctgactttt atcgggaagt gcgacgtaac
     6121 cccatcctgg ttcttaagcc agatgtactg atctttcttt aatccccaga tatctgccac
     6181 atcgggatgt atccacaccg agttttcttt catcaggtcg accaggttgg gattattggc
     6241 cgtccggctg aaagtgtgca tcggagcccg gccgtatatc agccggtaaa atccgggcgg
     6301 gggctcggga tgcgccgtgt aaaccggcat gggatcgaag ccgagcgatg ccagttctgt
     6361 gctgtaaagc tctatcctgc ctgaattggt attgaattcc atgggctggc cctcttccat
     6421 gtagagcgat ccggaagagc gttcaaattt tttaacaccc atacgctcca tttcttcgat
     6481 agacgttcca agctggttca actgccaggc cagcacttct ttgtaatcgg tataattgaa
     6541 ataagcgccc agtcccagcc gttctccaag ttgtttggca atccaccagg ccggtttgct
     6601 ttcgtacatc ggtgttgcgg ccggcatgcg gaatcccagg aatggctcgc gtgcaggaga
     6661 atcgcgcagt tcatcatacc gttccaggta ggtgcattcg ggcaatacca catcggcata
     6721 accggtgatc tccatgggca tcgtatcgac taccaccagc agatcgagcg cctgcatggc
     6781 tttgaggagc tgatcctttt gcggcatagt catcggcagg ttggtaccgc tgacgatcca
     6841 tccccggata gtatgttctt ttccttcggc aggaatggag gcatcgacga tggcgtttgc
     6901 gacggagagc ctcgccagag gatattttcc gtccagtgta tctttccatg accaggccgg
     6961 ttttggatat tccgggtgtg gaaagtcggg aatggaaagg ctctctttga aatagaaccc
     7021 tccgcggcga ccccaggagc ccagcaaagc gttgaggata gcaatggccc tggagcgctg
     7081 cgtgtcgtcg ccataccagg tgacatgcct tccgggatgc acgatgacgg caggggaggc
     7141 gttggccatg gcccgtgccg tttcgcggat aatgtgaggc ttgatcgtgg taattccgta
     7201 agcccattcc ggcgtcatct tttccacgtg ggccctgagc tgttcaagcc ccatcgtgta
     7261 tttttcgaca tagtccctgt cgtaaaggtt ttcagtgatg atcacattga tccaggcaag
     7321 caggagcgcc atgtcggtgg caggtttaat cggaagccag tacttcgatt tgctggctgc
     7381 ggtcgaaaag cgcgggtcga cggtaatgat cgtggcgcct ctgtcgatgg cgtccgacat
     7441 ctcctggacc tggccattgt gcatgttctc gccaagatga gatccgatca ggaccaggca
     7501 tcgggtatca cgtatatccg ttggctcggg tgaaccaaga ccgccgccaa atgtcagctc
     7561 gaagccaact tccctcggcc cgcggcattg cgcataagag ggagcggtga tgtttcccga
     7621 accataggct ttcagcacat gcccaaaata agtacccgat gaaccgtggt tgaacaaggc
     7681 cataccggcc gggccatgtt tagcagcgat ctgtttcatc ctgtcggcca ccaggttgag
     7741 tgcttcatcc caggaagctt cgcggaaaaa ctgccttccg ttttcttctg cccggataag
     7801 aggtgttttg agtcggtcgg gatcatggta catcccgggc ccgcccgtcc cccgcgggca
     7861 taaacgcccg ttgcaatgcg gatcctgctc atttccaatg attttctgaa tcttaccctc
     7921 ttcattcagg taaacccatc ccgcacattt ccaaaaacaa atttcgcaat atgtgggata
     7981 acgggttaat gagggtaaat ccgaagtggc tgcagattca ttattctcat ttttattgcc
     8041 ccaaacccat ttaatagcgc tcccggcaac ggccgcgcct ccgatcccta atgcggaaat
     8101 cttgataaaa tctcgcctgc ttttcattat atattgatta acagcaattt aagtataggt
     8161 ttttggatga cctttggttg ttttcgaagc aagcaaattt aatgaatata gatacgtaca
     8221 cataaagaaa gagggtttca ctctaattta gaatgtatat aaatacatat tataaattta
     8281 acccttccgg aaaattgaag tggatatcat tttcttttca gaatttagca tgatgagaat
     8341 ccgggaagca gacggaaagg gccggcgaag gtttatcctg actctgggaa cgcttatgct
     8401 gatccctttg gcaggcatct ggaatgccat ggcgaaaagg gcccaaaaaa gggaaggaac
     8461 tgttaaatct gtggttccaa tcgaagatat ttccggtgat gttttctatg cggaggagtt
     8521 cgttattatc agggaaagcg gagaattcac agtttattca acccggtgta cccatttggg
     8581 ttgccgcctt gagccggccg gtgatggcct tctcagatgt ccgtgccacg gttcggagtt
     8641 tgatctgaaa gacggaaata tccggaaagg gcctgcgctg aatccgttga aaacgcttat
     8701 ttttacaata cacgaaaaca atctgatcat ttatacctga aagatgatcc ggtttttcga
     8761 catagtgcaa aaatacctca acgacacggt cggcgggcta gccttatctt cgctgataat
     8821 ttgcattcta tccggtatct tcgtctcgct gccttacgat gccgcttctc cttttaattc
     8881 cctcagcctt atcatgatcg ataacccttc tgctgtctat ttccggaatg tgcattactg
     8941 gagcgcgcag ttattcctgg ctttgacctt attacatatc actgagcacc ttttgcggaa
     9001 atcggaaaag ttggtaacca gagctgtatg gtggaggctg accctttcgg tccctgtgat
     9061 cttttttgtg atgatcagtg gttttatcct caaaggggat gccgattccg tttcagcctt
     9121 cagggtattg tctgctttgt tccgggagat accgtgggtt ggatcccttt tatctttttc
     9181 cattaccgga aatgaacctt catttttaac gttgttcatg caccacattg ctacggcgac
     9241 gatcattatt gttgccgtta ttgcggaaca ttaccgaaaa ttctggccgc ctgcgggaag
     9301 ttttttcctg ctgacgtttt ttatattgat aatcagctat ttccttcatc cattttttgg
     9361 agtggagacg ggaaaaggcc cctggtattt tgtcggattg caggaattcc tgcgttattt
     9421 ttcaagacct gccttgatat gggttttggt cgctgttttt cttgccggcc tgggaattgt
     9481 ttcggcaacc gggccggttc agaacaggcg tataaagatt agtatagccg ttttctttct
     9541 tttctatggt ttacttaccg ttaccggttt gtttttcagg gatttcgacc aatccttgcg
     9601 atttccctgg aatattcatg acaaaggatg gatgggagtt gccatccggc cccttgcact
     9661 atcagtccct gaagatcatg atttttccgg accggttccc ctggttaacg ggcattacga
     9721 aggttgcctg gtgtgccatg acaatgtaaa gggattatcg gttgcacatg atccggcggc
     9781 cgtcggttgc tcatcctgcc atggcgggaa cccagctacc ctggataagc acttggcaca
     9841 caggaatatg atcctggttc ccgggcaact ggctgacgcc aggcgaagtt gcggaacagc
     9901 ttcatgccat cccggaattc cagaccggat tgaaggatcg cttatgacca ctatgagcgg
     9961 cgtaatttcg gtgaaccgat atgtttttgg cgaagcggcc acgccggata tccgggccca
    10021 tgtgaaagat gttggtaatt ccgcggctga caggcattta agggatcttt gcataaactg
    10081 ccacctgggc aatccgaaaa catatacggg gcccaataat tccatatcaa gaggaggggg
    10141 ttgcaatgcc tgccatctcc attattccga ttcggcgctc gtatcgcttg ctaactatcg
    10201 ccagtcggcg acaatagagg attccatttc tttttaccat ccacaactga acttaaaaat
    10261 tcccgatgat aattgctttt cctgtcacag ccggtcgggg aggattgcat tgagctatgc
    10321 cgggtggcat gaaacactct acaacgataa tgatattccg gatacgggta attttaaagt
    10381 cttagacgac cggagggttg ttcaatttgt gggccaggat gtgcaccata aagcggggat
    10441 gagctgtatc gactgccatc tttcccggga actgatgggc gatggggaat cataccttca
    10501 caaagaagaa caggtcaaga tcggttgcag cgattgccat ttcatggata atcctttgac
    10561 actatttgct aaggatgctg atccagagtc ggttaagact cttacattga gaaactggat
    10621 cgacaacagc ctccgattac tgaaaacggc aaccggtggt tatccgatca tcaattcgcg
    10681 ggtgagtgaa gacggcgggg cctggctggc gttgaaatcc ggcgattctt tgctggcgct
    10741 caggtcaccg gccgctgcct gcacgagggg taaggcacac cgttctctga gttgcgggag
    10801 ctgccactcc tcctgggttc ctcaatgtat cggttgccat aattcttttg atccggatgc
    10861 tcccggatat gacatgctgg agaaccggga aaggaccggt tcatgggtgg aatatgcggg
    10921 attgttcctc accgggaaac cggctttggg gataattgag gataagcgtg gaaacaggac
    10981 ggtaacaaca tttacaccgg gaatggtgat aagcattgat accggttctt acgatcaaaa
    11041 ccaggatggc ggccggatct ttcgccggtt gtacgcaccg gtatcagccc ataccacggt
    11101 caggcagggg aggccctgcc ggtcgtgcca cctcgatccg ctggccatcg gttatggccg
    11161 gggagagctg atatacagta tggacagaga ccgggggacc tgggattttg tgaaccggat
    11221 tgaacggagc ccctatgata acctgcccga agatgcctgg atcggtttcc tggaagaacc
    11281 tgacgcggct gtctccaccc ggtcgaatgc caggcctttc aacctggagg agcagaaatc
    11341 gatcctcaca gccggggcct gcctggaatg ccatgaggag aactcagctg tcatgcaggc
    11401 cgcactcgat aatttcgatt cggtaatgat taaagtttca aaaagatgcg ttttgcctgt
    11461 atggcctgcc gcgaattaaa tggcaagatc ttccggcgaa ttgatgcttt taaagtaaat
    11521 ctccggaagg ggtggatcta tttcggtgat aagcaggaga ctggtttcaa cccgggaaaa
    11581 gaggtccggc agcttgtagt ttttttcttc gaaaatggat cttgtcatcg tttcgagagt
    11641 gtttttccgg tacaaaccgc ataagggttc atatttctcc tgcccgtaat aagggatgca
    11701 tacctgtgct gtcccggccc ggtggatcag catctggaaa atattgccgg tgacaaaggg
    11761 catatcgcac gaaagcacaa ggtttatctc gtttgtgctt ttgagaaggc ccgagtaaag
    11821 ccctcccatc gggccgatct caccggtcac atccgggatc accgggtatc cgaactgctc
    11881 atactcgagg ttattcgaac tgatcaggat ttcgcggcac aacttttcga aaacgtcgat
    11941 agagtattgc accagaagtt ttccgtgaaa tgaaatcagg cccttgtcat ggcccatccg
    12001 gctgctccgg ccgcctgcca ggatgatccc cgtcatcatc gtccaggttc cgatttataa
    12061 aacaaacaac tgtcgccata gctcaggaac ctgaaattat tttcaagcgc ataccggtaa
    12121 gcctctttcc agtttttacc gataaacgac gagataagga gcaggagggt gctccggggc
    12181 tggtgaaaat tggtgatcag gatgtcggga taacggaacc ggtaaccggg agcgattatc
    12241 aggcttgttg cacccctgac cgaacctgcg cccattcgtt cgcaaccgct gataacggta
    12301 tccagcgatt cttccctgga tattttaccc ggtaccggca gatcgtaagg atcccattgc
    12361 ctgacatcca ggacagacgg ttcagacaaa cccatgatca ccttaacgcc atgccagtaa
    12421 aggctttcca ggaggcgggt cgtggtggtg ccgactgcgg tgatcttcct gtccagatta
    12481 ccacgaagat cttttaatgc taacaggggc agatcaacct gttcggcatg catttcgtgg
    12541 ccctccaggc gttccgacga caccggccgg aaggtgcctg ctcctacatg caataccagt
    12601 ctttgtgatt taatcccctt agcttctaac gatgcgagga cttccggtga aaaatgcaga
    12661 ccggccgttg gagcggctac ggaaccctcc ctgctggcat aaatcgtctg gtaggtgcgc
    12721 tcgtcattat gtaccggatc ccggtgaata taggggggaa gagggatctt tccggcttgt
    12781 tccaggatat gagaaaacgt caggtgttcc ggatgccagg taaaacggat aacggaagat
    12841 ccgtcttcgt ttcggctggt cgtttctgcc ttcaggacaa attgatcctt gccctctccg
    12901 gctgtcagct ccagtccgcc gtgtttccat cttttggagt tgccgaccag gcaaagccag
    12961 tcggcctgcc cggtgcatcc gaaggcccgc aggtgatcgc ggtatggcgc cacaggttca
    13021 aggcagaaaa gttcaatccg gctgccggtc ggacggcgga acagcaaccg ggcatggatc
    13081 acccgggttt cattgaaaac caccaggctg tcgggaggca aatgggtggc catgttgctg
    13141 aaaatatcct caccggtacg accgttacga accagtaaca gtttcgacga atcccgctgc
    13201 tcaaggggaa acagggctat ccggtcatcc ggcaggggat aatcgtagtc ttcaatgcgc
    13261 aggtttcggg gatgcaggtt gatggccacg ggtctgtcct taatttttcc acaaagatat
    13321 aaaacagtta atctttcgta cttttgccgt tagtcggtat caaggcctga ggcttgccgc
    13381 cctgcagcga tgaattttaa aaagaatatc cccaacggaa ttacactcct gaacctggtc
    13441 agtggcggag tctccgtgat catggccctc tcgggcgaac ctgcctgtgc tgcctggctg
    13501 atcctgctgg ccgcagtctt tgactttctt gacggattag ccgcccgttt gctgaatgcc
    13561 cgatcggcaa taggcggcga actcgattcg ctggccgatg tgatctcgtt cggactcgcc
    13621 ccgtctgcca ttctttatgc gctgatgctg aaaagcccga acctgcctga gtttgctgcc
    13681 ggtgaatacc agttattccc gttttcagcg cttctactgg ctgcttttgc cgctgccagg
    13741 ctggcaaagt tcaataccga tccgggacag gcgatcgagt tcagaggatt gccgacgccg
    13801 gcgaccggcc tgttcgtcgc tgctttcccg ctgatggtgc attaccaggg ggacatggaa
    13861 tatgtcagat tgttaaccgg aaatttttat ctgcttctgg ctgtggtaat cgtgctgtcg
    13921 gcgctgatgg tctcttc
//
LOCUS       DAOPNH010000048        13932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4435_length_13932_cov_7.450962, whole genome shotgun sequence.
ACCESSION   DAOPNH010000048 DAOPNH010000000
VERSION     DAOPNH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_13932_cov_7.450962"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKZ74_03990"
     CDS             complement(<1..453)
                     /locus_tag="PKZ74_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM91949.1"
                     /translation="MNKKVRIARLSIASNTLLIIMKFIVGMISGSVSILSEAIHSLMD
                     LVAAVIAYFSVRVSDNPPDKRHPYGHGKFENLSGVLEALLILVAAAWIVYEATRKMTG
                     KEPVDSIGLGSLVMFISAGVNIFVSRKLYKVAKETDSVALEADALHLKT"
     gene            complement(522..1574)
                     /locus_tag="PKZ74_03995"
     CDS             complement(522..1574)
                     /locus_tag="PKZ74_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPM91950.1"
                     /translation="MKKIKHIGIVGEGKMGSSIFLFLLGYDFRLTWLCSGSMGKEKAA
                     KQAAKKINILLKSGILTDAAFRKKNEETKITETPGDLADCDLVIEAISEDLETKKEFF
                     RNLDLMVGRKCIFTTNSSSINPSMLIPSESRQERFAGMHFFFPVNLKNTVELIQGPDT
                     SEETVGILKDFLRLIHKNIFMQDEKHAFILNRLLLDFQAEAFNICKDGDISYRQMDDL
                     VKERFFPAGVFEFFDHVGIDVMLASIRNYIKNEPDKSLYLPIVARMKELIKNNELGLK
                     TRKGFYDYELQGRPCDDDEMQNILPAGRLGEIEKRLRQRLEGAYRSVLATGCVSAETF
                     DEAVKDYLGTDQCLPW"
     gene            complement(1589..2707)
                     /locus_tag="PKZ74_04000"
     CDS             complement(1589..2707)
                     /locus_tag="PKZ74_04000"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HPM91951.1"
                     /translation="MSHILPETIRVAGTGSYLPGRPLSIDEAEQVLGELHEAPEKIRA
                     WMKRLKMMMKELLEIERYYYAIDPVTRQFTDDNITMSVKAAQRALEMAGKTPGDVEFI
                     VYGSAHMDQMPTPSVRIQEALGISRCAETSVHANCTSAYKALLIAHELIRSGRYRNTL
                     VISSGISSSELRAEYYNQAVVKKEELFLRYYLGDGAGAVFLEAGNGEEGLYLESTYME
                     SVGGDKPSAMNNRRPAYWMNPKEEYEKGYHHLAQMFRDELARNFHESGGSVFLHGLER
                     YLGKYPADLSKLKYFQVNFPSRHISELIMEECETLGIAKEKLYSKMSGLGYPGPPMAF
                     ICLDNILRNEKLNPGDLVLSFVTEVSKFMQAGYLMEYR"
     gene            complement(2704..3339)
                     /locus_tag="PKZ74_04005"
     CDS             complement(2704..3339)
                     /locus_tag="PKZ74_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPM91952.1"
                     /translation="MELKTIRWITEDGIGQLALDQPPSNTMDRRFFDELSLLTRMVIP
                     GSGIRALVVYGTGRHFSSGADPADLCRRVRETLPSNYPQEIPAFLSMARDCFQYIEKL
                     PVPTFAAIRGVCLGSALELALACRFRICAEGAVFGFPESSFGLMPGCGGTVMLTKAAG
                     SQKAIELLLGGRNFSAGEALEMGLVHRIVSRKTVVEETLKIAKGMISTLSL"
     gene            complement(3329..3706)
                     /locus_tag="PKZ74_04010"
     CDS             complement(3329..3706)
                     /locus_tag="PKZ74_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91953.1"
                     /translation="METITEKNIFTDIISREDFILLFSSSEDKENWFTEKELETIGRK
                     GLSGSLAARYLIRKRIAYQVGINIAVTEIEILNDPSGKPEIRFSERWHTALSKAGFQS
                     VVCSLSHSRNFITSLTIFLSNGA"
     gene            complement(3706..4443)
                     /locus_tag="PKZ74_04015"
     CDS             complement(3706..4443)
                     /locus_tag="PKZ74_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPM91954.1"
                     /translation="MEVSWEKIWERSGSIGILELQGPKGNQLEEPAFLPLPLLEELFG
                     EPGLKGVIIRGSGRHFSAGANLEHLKKLALDEVLLAEKMTAGKETIRYIESSDLPVVA
                     EISGICFGGGLEIALACHFRICSENALFAFPEINHGLMPGLGGTVMLSRLIGQAKAAG
                     MILSGDTVDAEKAMALGLADYVLPAAGLHAFTLDFLQKLTDDRDPGVIRSIMRSIHNA
                     REMDTESALAEETRLFCALAVNNFHSG"
     gene            complement(4443..4901)
                     /locus_tag="PKZ74_04020"
     CDS             complement(4443..4901)
                     /locus_tag="PKZ74_04020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPM91955.1"
                     /translation="MRHPHYKRTRYNGFSRRTTEKSEKMFEYNFSVRGYELDSYGHVN
                     HAVYLNYLEQARWELFRNLGLLGYFHENDLLLVVIDVHMRYSREISLFDEVIVRTGIT
                     SEPPYLVFHHKMYLKGSNLKICSATIKTLFTDKGKLVRDIPKSITDKLIT"
     gene            5258..6472
                     /locus_tag="PKZ74_04025"
     CDS             5258..6472
                     /locus_tag="PKZ74_04025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM91956.1"
                     /translation="MPKKVVITGMSLITSLGLDLQTNWDNLIAGRNGVKHISLFDPTD
                     LQTQVAAQVSEDFEACSTKYLKKRTADQMTRVTRMAFVCATDAIKDAGVDFEAQDRTR
                     CGVILGVVSTGNTSAEKGTTARNRVLKSMNNAMPAWISLEHKLMGPNFTVSSACSSSA
                     YAIGLGYDLIRNGAADIVITGGADSIINREEIEGFNELYALSVNNDPETACRPFSLDR
                     DGFVIGEGAGILVLESEESAKKRDARIYAELAGYAITSEAYNIMAPMKDGEGICHTIH
                     EALKNAGVKPEEVEYINAHGTSTILNDRYETMAIKKVFGEGAYRIPVSSTKSMIGHTV
                     GAAGAIEGIVTILSIYHDVITPTIHLDQPDPELDLDYVPHTSRNQLVNCALSNSFAFG
                     GHNATLVFKKYA"
     gene            6509..6814
                     /locus_tag="PKZ74_04030"
     CDS             6509..6814
                     /locus_tag="PKZ74_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM91957.1"
                     /translation="MIEITDEIRSDVREIVCQFFAEQCEVEIGSINDQTNIITDLEGD
                     SLMFLELIEIFKKKYNLDIELKTVGKYVLKHPVETIGQLIETQLLMIQYGNQILEMK"
     gene            6825..7223
                     /locus_tag="PKZ74_04035"
     CDS             6825..7223
                     /locus_tag="PKZ74_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPM91958.1"
                     /translation="MSEIAGCGIDIEELARFSRHLPVNESIPDLWHMISTEEEISINR
                     QVSPHVSFPLSFSCKEAMFKALGRSWTNSNISWKEIELIFQDHQLGGDFLIRLSGHAL
                     ERYRALNCTRIESAFSFTDDYVIFQIILHS"
     gene            7328..8161
                     /locus_tag="PKZ74_04040"
     CDS             7328..8161
                     /locus_tag="PKZ74_04040"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPM91959.1"
                     /translation="MVALASDTLDTDLTRFDFAENPMLNDELKSQYIAYIYSCAVADG
                     LLAQNIAPVHVSGYSMGIYAALYYCRAIRFTDGLFLIRSAWEKISEATDDNRYGMGMI
                     IGLKEDDITRFRGYGENVEICNRNNPYTYIISGKRDAVEAILSASLEEGALRASLLPV
                     SMPYHSMILHTASTRFGDAIRQMEIKNGKPVYFSALSQEEIITGDGLRRELIRNIFSR
                     MNWYETMKSLIEKGVTAFYECGAGDGLTKNFRFIGGSFKAYPVDKYRQFIDDPEAISL
                     R"
     gene            complement(8154..9494)
                     /locus_tag="PKZ74_04045"
     CDS             complement(8154..9494)
                     /locus_tag="PKZ74_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM91960.1"
                     /translation="MKILLVNPPRSPENKILEYAPADARPFIHKKLIGPPLGLLTIAA
                     AVKDFDVTVIDLKGEYDLDPDAPPLEQLVLGYLAEYQPDIVGVTFIASELYAGLDIFR
                     TAKKFNPEIITVAGGLHVTICPEDFADPCVDILCPGHSAGIFRNLVIHFDKGLPPDHT
                     AGIILNEHGTLRRTAKPASDWDPAGKDFIHPDRSHLTRWLQTYKVGNDPTPVTYIFTS
                     LGCPFECSFCAIWPQHDKQYYIREVESVIEELKGLDDYGVVRFADANTIVDIRFIDKL
                     FDRILEEGIVKKYVMDIRPDTAARHPELIEKLARAGLKVVICGFESFRDEELDKYNKR
                     SAASNIKKSIEVFHRNDIMIRGNYIVPNDYGEEDFKALAAYSASNPVVYAGYTILTPM
                     PGTVYYREVKDQIVDHDLSKYNFFNSVMKTKLPPDQFYESVGSLWKIKLGKDVI"
     gene            complement(9511..10158)
                     /locus_tag="PKZ74_04050"
     CDS             complement(9511..10158)
                     /locus_tag="PKZ74_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91961.1"
                     /translation="MKRASAFLIVLLSGLFIVSCQKPADVTSNPAASGIEKTGNPFVT
                     PEARQALVNTLREVAQEQEAIRGAHFLPAFYTDWGFGFLDDDMVRLASFSTVIDENDF
                     FRENPDGTVTVHIQSETAFSELFNYSTYDYYFGSNGHMVMNYTGPVMMIPIFDNNGNF
                     LGFIYLVQPDNSSPASVWHGNGPVQLWGMGQEYNLVAKLIANAGWKKVNKSIKLN"
     gene            complement(10345..10417)
                     /locus_tag="PKZ74_04055"
     tRNA            complement(10345..10417)
                     /locus_tag="PKZ74_04055"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10382..10384),aa:Gln,seq:ctg)
     gene            complement(10474..>13932)
                     /locus_tag="PKZ74_04060"
     CDS             complement(10474..>13932)
                     /locus_tag="PKZ74_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91962.1"
                     /translation="TIYQGEDASTRISGFSITNGKGNSGGGIRIFESDPYLDHLNIYG
                     NSASSGGGIYAYWCQPAISNCLIQGNTATEQGGGIWLGTDSYSWDSPQLTDLVIKDNN
                     AGYGGGIAMDFYIGGYFNGITVCHNTAGTGGGILFGYYGNVVFNPEHRCNIYQNSAAV
                     GNDLYSHANVDVIVDTFTVMNPTEFHASEIEEFTFDILNSKLEQVEYDLYISPEGDNN
                     NSGLTADDPLRTIHHALTILLASESGQRQIHLLDGIYSPETNGEFFPVSLPDYVHLSG
                     TADSLVILDADSTDRVMQIRDNDTNYVSDLTLCGGLAESGAGIYIYYSDPCLERLIIR
                     DNSSSVGNYSKSGGGISGDHSEAILKDIIIAGNYAQSSGGGLYFTGSSPVLQNICILN
                     NTAGSGGGLYLSTSTERTICDQLSVLENHASNGAGILNYGFLELRNSIINANVSGNAG
                     GGIYSRDSIWIFNSNLVNNTSGSGGGLYVYANSYALLDSSELSGNHAIGGGGGLCCGT
                     ETVVYLNHVKINSNLSNNTAGGIFTATTGLHIKKSDITYNHADNYGGGIYCDDEAFPQ
                     FDSTDRCNLFLNWAGTAKDIFTKDGMVNVILDTFTVLHPTDYFVNDPDDFTFDILAGK
                     VEQAYADLYVSTVGSDTNSGFSSDNPLKTIDRALMILRSDSLNQHSIHLADGIYSGSS
                     NGEKFPLQLSDYCSLEGETRDGTILDAEDSSHVLNLYHDHIPEIKNLTLTGGHGMNGG
                     GLLGYRSVFNLFNVKIRDNTSNYQGGGVYVGSGSIAELNHVVFENNESGTGGGIDISY
                     SSAHLKNIDFTHNQATDGGGIYISYPDTLTMDHIYCFSNTAENYGGAIYARNSDFILA
                     NAVFDSNQAYRGGGFCLESSSMDAKNTLFVRNTAADGYGGAGTIYNYGAPPDAEVNFY
                     NATCTQNTPPVLHIYGGKASLTNSILWDNNNPIQIYYFVDYSESDTLTISYSDIQSAQ
                     AGIFKSGGAVLDWNAGNINEDPQFSMGDEYPFSLDAGSPCIDAGTPDTTGLLLPYNDL
                     IGNVRVWDGDADGISIIDMGPYEYGAPVTVDDSSFPEAGKKIHFTLYPNPAKDHLTIQ
                     CSDHRGDIIIKLINYSGQIVKVITLPAGLNMTHIPTDHLGTGAYWVLFTDEEGHSTIE
                     KLIISK"
BASE COUNT         3602 a         3406 c         3332 g         3592 t
ORIGIN      
        1 ggtcttgagg tgcagggcgt ctgcttcaag ggccacagaa tcggtctctt tggctacttt
       61 atacagcttc cttgacacaa aaatatttac cccggccgag atgaacataa ccaatgatcc
      121 aagcccgatc gaatccacag gttccttgcc ggtcatttta cgggttgctt cataaacgat
      181 ccaggcggcg gcgacaagaa tcagcaatgc ttcaagaacg ccggaaaggt tttcgaattt
      241 tccatggcca taggggtgcc gtttatcggg aggattgtcg gatactcgaa ccgagaaata
      301 ggcaataact gctgcaacca ggtccatcag agaatgtatg gcttccgaca aaatactgac
      361 agaaccgctg atcataccta caataaactt catgataatc aataaagtat tggaagcgat
      421 ggaaagacgg gctattctga ctttcttatt cattaagaat aaattttaag tcaaatgtac
      481 tattaataaa aggcaaggcg agagtgtgtt tttaccaatt tttaccaggg aagacactga
      541 tctgtaccca gataatcttt tacagcctca tcaaatgttt ctgctgacac gcaacccgta
      601 gcaagcacac ttctgtaagc cccctccagc ctttgcctga gtcgtttttc aatctcacct
      661 aaccttccag caggtaaaat attttgcatc tcatcatcat cgcaaggccg gccctgaagt
      721 tcgtagtcat agaatccctt ccgggttttt aatccaagtt cattattttt gatcagctct
      781 ttcatccggg ccactatcgg gaggtaaaga cttttatccg gttcattttt aatgtaattc
      841 cggatggagg ccagcatgac atcgatgccc acatggtcga aaaattcaaa gaccccggcc
      901 gggaaaaatc gctccttcac gagatcatcc atctggcggt acgaaatgtc gccgtcttta
      961 caaatattaa atgcttcggc ctggaaatca aggagaagac ggttcaggat aaacgcatgt
     1021 ttttcatcct gcatgaaaat atttttatga ataagtctta gaaaatcctt cagtatgcca
     1081 acggtttctt ccgaagtatc aggaccctga atcaactcga ccgtgttctt caaatttacc
     1141 ggaaagaaga aatgcatacc tgcaaaccgc tcctgcctgg attcggaagg gattaacatg
     1201 gatgggttta tggaggatga attcgttgta aaaatgcatt tccgacccac catgaggtca
     1261 aggttgcgga aaaattcctt cttcgtttcc agatcttcgg aaatggcttc aatcaccagg
     1321 tcgcaatcgg ccaggtcgcc aggtgtttct gttattttcg tctcttcgtt ttttttccgg
     1381 aaagccgcat cggtcaggat cccgcttttc agtaagatat tgatcttttt cgccgcctgc
     1441 ttcgctgcct tctctttgcc catactgccg ctgcaaagcc aggtaagacg gaaatcatac
     1501 cccaggagaa acaggaatat ggatgacccc atctttcctt ctccaacaat gccaatatgt
     1561 tttatttttt tcaattaaaa agtcaaattt aacggtattc catcagataa cccgcctgca
     1621 tgaatttaga cacttcggtg acaaaactca ggaccaggtc gcccgggttg agtttttcgt
     1681 tcctgaggat attgtcgagg cagataaacg ccatcggcgg tccgggataa cccagacctg
     1741 acattttcga atacagtttt tctttagcga tgccgagcgt ctcacattcc tccatgatca
     1801 gttcggagat gtgacgggaa gggaagttga cctggaaata tttcagtttt gaaagatccg
     1861 ccgggtattt tccgaggtac cgctccagcc cgtgaaggaa aacacttccc ccggattcat
     1921 gaaagttgcg ggccagctcg tcgcgaaaca tctgcgcgag gtgatggtaa cctttctcgt
     1981 actcctcctt cgggttcatc cagtaggcgg gccggcggtt gttcatggcg ctgggcttgt
     2041 cgccaccgac cgattccata taggtgctct ccaggtaaag cccttcttct ccgtttcctg
     2101 cctcaagaaa cacagccccc gctccgtcgc ccaaatagta ccttaaaaag agctcttcct
     2161 ttttcaccac cgcctggttg taatattcgg ccctgagctc cgacgacgaa atacccgaag
     2221 agatgaccag cgtgttccgg tacctcccgc tccggatgag ttcgtgggcg atgagcagcg
     2281 ctttgtaagc ggaggtgcag ttggcatgga ccgacgtctc agcacaccgg cttatgccaa
     2341 gcgcttcctg gatcctgacc gatggcgtcg gcatctggtc catatgggcg ctgccgtaaa
     2401 cgataaattc cacgtcaccg ggagttttgc ccgccatttc cagtgcacgt tgcgccgctt
     2461 tcacactcat ggtgatgttg tcatcggtga attgccgcgt taccggatcg atggcatagt
     2521 agtaccgttc gatctccagc aattctttca tcatcatctt caggcgcttc atccaggccc
     2581 tgatcttttc gggtgcttca tgcaattcgc cgaggacctg ttcggcctca tcgatcgaca
     2641 ggggcctgcc gggcaggtag ctgccggtgc cggcgacacg aatggtttcc ggtaggatgt
     2701 gactcataat gatagtgttg aaatcattcc ctttgctatt tttaatgtct cttccacgac
     2761 ggtttttctc gaaacgatcc ggtggaccag gcccatctcc agggcctcgc cggcggagaa
     2821 attccgtccg cccaggagca gttcgatggc tttttgactg cccgctgctt tggtcagcat
     2881 gaccgttccg ccgcaaccag gcatcaaacc gaaggagctt tccgggaacc cgaaaaccgc
     2941 accctctgca caaatcctga accggcaggc cagggccagc tccagcgccg atccgagaca
     3001 gacgccccgg atcgcagcga atgtcggtac cggaagtttt tctatgtatt gaaaacaatc
     3061 acgtgccatg gagagaaaag ccgggatctc ctgcgggtag ttagacggaa gggtttccct
     3121 tacccgccgg cacaggtcgg caggatcagc gcccgaggaa aaatgtcggc ccgttccata
     3181 aaccaccagg gccctgatcc ctgaaccggg aatgaccatc cgggtcagca ggctcaactc
     3241 gtcgaaaaac cggcggtcca tggtgttcga cggcggctgg tcaagcgcca gttggccgat
     3301 gccatcttct gttatccaac ggatggtctt aagctccatt gctgagaaaa atagtcaggc
     3361 tggtgataaa attccgggaa tgcgaaagag agcagacaac agattggaaa ccggccttgc
     3421 tgagggccgt atgccaccgc tcactgaacc ggatctccgg tttgccagaa ggatcgttca
     3481 gaatctctat ttccgtcacc gcgatattta ttccgacctg gtaagcaatc cttttcctga
     3541 tgaggtaacg ggcggccagg ctaccggata accctttcct gccaatggtt tccaattcct
     3601 tttccgtaaa ccagttttcc ttatcttccg aagatgaaaa cagaaggata aaatcttccc
     3661 tggatatgat atcggtgaaa atgttttttt ccgtgatagt ctccatcagc ctgaatgaaa
     3721 attattgacc gccagagcgc agaaaagccg ggtctcctcc gccagggcgc tttccgtatc
     3781 catttcccgg gcattgtgaa tggatctcat gatggaacgg atcactccgg gatcacggtc
     3841 atctgttaat ttttgaagaa aatcaagggt gaaggcatga agaccggcag caggcaaaac
     3901 ataatcggca aggcccagcg ccatcgcttt ctcagcatcg acagtgtcac ccgaaaggat
     3961 cattccggcc gccttagcct ggccgatcag ccgggaaagc atgaccgttc cgcccagacc
     4021 cggcatcagg ccgtggttga tctcggggaa tgcaaaaagg gcgttctccg agcaaatcct
     4081 gaaatggcag gccagtgcga tctccagtcc gccaccgaag caaatcccgc tgatctccgc
     4141 aacaacaggt aaatccgatg attcgatata ccggatggtc tccttgccgg cggtcatctt
     4201 ctccgccaga aggacttcgt ccagagccag tttctttaag tgttccaggt tggcgcctgc
     4261 cgaaaaatga cgcccgcttc cccggatgat cacccctttc aaaccgggtt cgccgaatag
     4321 ctcttccagc aatggcaacg gcaaaaatgc cggttcttcc agctgatttc ctttgggccc
     4381 ctgcaactcc aggatcccga tcgaccccga tctttcccat atcttttccc atgaaacttc
     4441 catcaggtga tcagtttatc ggtaatgctt ttcgggatat cccttaccag tttccccttg
     4501 tcggtaaaaa gtgtcttaat ggttgcagag cagatcttca ggttcgatcc ttttaaatac
     4561 atcttatgat gaaaaacaag ataaggaggt tcacttgtaa tcccggtcct gacgatcact
     4621 tcgtcgaaaa gtgaaatttc ccggctgtac ctcatatgca catcgatcac cacaagcaac
     4681 aggtcgtttt catgaaaata accgaggagg ccgaggttcc ggaatagctc ccatcgcgcc
     4741 tgctcaagat aattcaggta aacggcatgg ttcacatgac cgtatgaatc aagctcataa
     4801 cctcttacgc tgaaattata ttcgaacatc ttttcacttt tttcggtagt ccgacgactg
     4861 aagccgttgt atcgtgtacg tttgtagtga gggtgtctca aaaatctgac atttcgattt
     4921 gattacctga agacagttcg tgctccaacg actaaagccg ttggttccga atgttttatt
     4981 agggttggaa ccgacggctt cagtcgtcgg actaccttta aaaagtgaat atatggactt
     5041 ttgagacagc ctcttcattt ttttaacctg ttcggaacca acggctttag tagttggagc
     5101 actaattaaa gctgaattat tccacatagt gaataatttc attttcagct caaaaataat
     5161 tttaaagaat ttccccgtac aaaaatagaa ggaaagtttt atttattttg ccagaagttg
     5221 aaaacggaat catttactca atcataaaac cggaaaaatg ccgaaaaaag tggtcattac
     5281 gggaatgagc ctgattactt cgctgggatt ggatcttcag acaaactggg ataatctgat
     5341 agcgggcagg aacggcgtta aacacatttc gctgtttgat cccacggatt tgcaaacgca
     5401 ggttgcagcg caggtttccg aagattttga ggcttgctcg acaaaatacc tgaagaagcg
     5461 gaccgccgac cagatgacaa gggtcacccg gatggcattt gtatgcgcaa ccgacgcaat
     5521 aaaagatgcc ggagtggatt ttgaggctca ggacaggacg aggtgcggtg tcattctcgg
     5581 tgtggtaagt accggtaaca catccgccga aaaaggtacg acggcacgga accgggtgct
     5641 aaaaagcatg aacaatgcca tgccggcctg gatctctctg gagcacaaac tgatggggcc
     5701 taattttacc gtatcctctg cctgctcttc gtcggcatat gccattggcc tcggatacga
     5761 cctgatccgc aacggcgcgg ccgatatcgt gatcaccggg ggggccgact ctatcatcaa
     5821 ccgcgaagag atcgagggat tcaacgaact gtatgccctg tcggtaaaca atgaccccga
     5881 aaccgcctgc cgtccctttt cgcttgaccg cgacgggttt gtcatcgggg aaggcgccgg
     5941 tatccttgtt ctcgaatcgg aagagtcggc taaaaaacga gatgcccgga tttacgccga
     6001 gctggccggg tatgccataa ccagcgaagc ttacaacatc atggccccca tgaaagacgg
     6061 ggaaggtatc tgccacacca tccatgaggc gttaaaaaat gccggcgtaa aaccggagga
     6121 agtggagtat atcaatgccc acgggacgtc gacgattctt aacgaccgct acgaaaccat
     6181 ggccattaaa aaggttttcg gagaaggggc ttaccggatt cccgtttcat cgacaaagtc
     6241 gatgatcggg catactgtgg gagctgccgg ggccattgaa gggatcgtga ccattctgag
     6301 catttatcat gacgtgatca cgccaacgat ccatctcgat cagccggatc ctgaactgga
     6361 cctcgactat gtcccgcaca cttcgcgcaa ccagttggta aactgtgcct tatccaattc
     6421 atttgccttc gggggacata atgccacgct ggtatttaag aaatatgcct aattttaccc
     6481 tcttaatccc aaaaaacata acctgtatat gatcgaaata acagatgaaa tcagaagtga
     6541 tgtacgagaa atagtctgcc agttctttgc cgaacagtgt gaagtggaga tcggctccat
     6601 taacgaccag accaacatca tcaccgatct cgaaggcgat tcgctcatgt tcctggaatt
     6661 gatcgaaatc tttaaaaaga aatacaatct cgatatcgaa ctgaaaaccg tcgggaaata
     6721 tgtcctgaaa cacccggtgg aaaccatcgg acaattgatc gaaacccagt tgctgatgat
     6781 acaatacggg aaccagattt tggaaatgaa atagttgcaa cccgatgagt gaaattgccg
     6841 gatgtgggat cgatattgaa gagctggcac ggttctccag gcatttaccc gttaacgaat
     6901 ccattcctga tctatggcac atgatctcta ccgaagagga gatcagtatc aaccggcagg
     6961 tttcgcccca cgtttcattt cccctctcat tttcctgtaa ggaagccatg ttcaaagccc
     7021 tcggaaggag ctggaccaac agcaatattt cctggaaaga gatcgaactg atctttcagg
     7081 atcatcaact cgggggagat tttctcatcc ggctgtccgg tcatgccctg gaacgttacc
     7141 gggctttaaa ctgcacgaga atcgaatccg ctttctcctt cacggatgac tatgtaattt
     7201 ttcagataat tttacactct taaaatctgg cgttatcgga acctgtatca tttttcctgc
     7261 atttccgggt gagtataccg gcaaagaggg ccaggcgctc cggggccggg aagcccattt
     7321 ccgccagttg gtcgccctgg cctccgatac cctggataca gatcttacca ggtttgattt
     7381 tgctgaaaac ccgatgctga atgacgagct gaaatcacag tatatcgctt atatctatag
     7441 ttgtgccgtc gctgatggac ttttggcaca gaatatagcc cccgtgcatg tttcgggcta
     7501 ttccatgggc atttacgccg cgctttatta ttgcagggcc atccggttca ccgacgggct
     7561 ttttttgatc cgatcggcct gggagaagat ttcggaggca acggatgata accgttacgg
     7621 aatgggtatg atcatcggtt tgaaagaaga tgatattacc cgtttccggg gatatgggga
     7681 gaatgtcgaa atctgtaaca ggaataatcc ctacacctat atcatctctg gcaaacggga
     7741 tgccgtggag gccattttat ctgcatcgct cgaagaaggt gcgctccggg ccagcctgct
     7801 gccggtttcg atgccgtatc actccatgat cctgcatacg gcatccaccc gtttcggaga
     7861 tgccatccgc cagatggaga taaaaaatgg caaaccggtt tatttttcag cgttaagcca
     7921 ggaagaaatt atcaccgggg atggcctcag gagggagctt atccgcaata ttttcagccg
     7981 gatgaattgg tatgaaacga tgaaatcact gatagaaaag ggagtaacgg ctttttatga
     8041 gtgcggcgcc ggcgacgggc tgacaaagaa tttcaggttt atcgggggta gtttcaaggc
     8101 gtatccggtg gataaatacc ggcaatttat tgatgatcct gaagctatat cactcagatg
     8161 acatcttttc ccagtttgat cttccataaa cttccgacgc tttcatagaa ttgatccggc
     8221 ggaagttttg tcttcatcac actattgaaa aaattgtact tcgacaaatc gtggtcgacg
     8281 atctggtctt taacctcacg ataataaacc gttccgggca taggtgtgag gatggtataa
     8341 ccggcataaa cgaccggatt tgaggcggaa taagcggcca gggccttgaa atcttcttcg
     8401 ccgtaatcat tcggaacgat gtagttgccg cggatcatga tgtcgttgcg gtgaaagact
     8461 tcgatcgatt ttttaatgtt ggaagcggct gagcgcttgt tgtacttatc cagttcttca
     8521 tcgcggaacg attcgaatcc gcagatgacg actttcagcc ccgcccgggc cagtttttcg
     8581 atcagttcgg gatgcctggc tgccgtgtcg ggacggatat ccatgacgta ctttttcacg
     8641 atcccctctt caaggatgcg gtcgaacagt ttgtcgatga accggatatc gacgatggtg
     8701 ttggcatcgg caaaccgtac cacgccatag tcgtcgagtc ctttcagttc ttcgatcacc
     8761 gattccacct cgcggatgta atattgcttg tcgtgctggg gccagatggc gcagaaggag
     8821 cattcaaaag ggcatccgag ggaagtgaag atatacgtga cgggagtcgg gtcgttgccc
     8881 accttatagg tttgcagcca gcgggtgagg tggctccggt cggggtggat gaaatccttg
     8941 ccggcgggat cccagtcgga ggccggttta gcggtccggc gaagcgtacc atgctcattg
     9001 agaatgatcc ctgccgtatg atcaggaggt aatcctttgt caaagtggat caccaggttg
     9061 cggaagatgc cggcggaatg ccccgggcac aggatatcca cgcaaggatc ggcgaagtct
     9121 tccgggcaga tcgtcacatg cagaccgccg gcgacggtga ttatctccgg gttgaatttt
     9181 tttgcagtcc ggaagatatc caggcctgca tagagctccg atgctatgaa ggtcactccg
     9241 acaatatcgg gctgatattc agccagataa cccagaacca gttgttcaag cggtggggca
     9301 tccggatcga ggtcatattc ccccttcaga tcgatgacgg ttacatcgaa atctttaacg
     9361 gcggctgcaa tggtcaataa tcccagcgga ggcccgatca gtttcttatg tataaacggc
     9421 cgggcatcgg cgggagcata ctccaggatt ttattctcag gagaccgggg tggattgaca
     9481 aggaggatct tcaaaccagg agacccgtca tcaattgagt ttaatgctct tgttcacctt
     9541 tttccagccc gcgttggcga tcagtttggc caccaggttg tattcctggc ccatccccca
     9601 gagctgcacc ggtccgttgc cgtgccaaac gcttgccggg ctggagttat caggctgcac
     9661 caggtagatg aacccgagga aatttccgtt attgtcgaaa atagggatca tcatgaccgg
     9721 gccggtgtaa ttcatcacca tatggccgtt cgaaccgaaa taataatcat aggtggaata
     9781 attgaacagt tcgctgaaag ccgtttccga ctggatgtgg acggtgaccg taccgtcggg
     9841 gttttcccgg aagaaatcgt tttcatcgat taccgtagag aaggaggcga gcctgaccat
     9901 gtcatcatcc aggaacccga atccccaatc cgtatagaag gccgggagga aatgagcgcc
     9961 acggatcgct tcctgttcct gtgcaacttc acggagagtg ttgacaagcg cttggcgtgc
    10021 ttccggcgtg acaaacgggt tgcctgtttt ttcgattccg gacgccgccg gattagaagt
    10081 tacgtcggcc ggtttctggc acgatacgat aaatagtcct gataataaaa cgattaaaaa
    10141 tgcgctggcc cttttcatag tcgtagtttt aggttatcgg cccaaaaata gattaaaata
    10201 tggctattta tgctttcgat ttcttaaatc attaacaatg aaatgttaaa aggatgggtt
    10261 ggctggaaag aaaaaaaaaa ttcgcaatac ttgttattgt aattgcgaaa tgatattgtg
    10321 aaatgatatt gtgaaatgaa ggtgttgccc gatcaggggt cgaacctgaa ctcttctgat
    10381 ccagagtcag acgtgttgcc agttacacca tcgggcaatg agagcgcaaa aatagtaaat
    10441 tttgaatgtt caggatcgaa tatagaatta ttttcatttg gaaatgatca gtttttcaat
    10501 ggtagaatgg ccttcttcat ccgtaaacag tacccaatag gcccctgttc ccaggtgatc
    10561 tgtcggaata tgcgtcatat tcaggccagc cggaagagtt ataaccttaa caatctgacc
    10621 ggagtaattt ataagcttaa tgattatatc tccccggtga tcagaacatt gtatggtcag
    10681 atgatctttg gcaggatttg gatagagtgt aaaatggatc ttctttccgg cttccgggaa
    10741 tgaggaatca tcaaccgtta caggtgctcc gtactcgtac ggacccatgt cgatgatgct
    10801 tatcccatct gcatcgccgt cccataccct gacattaccg atcagatcat tataaggcaa
    10861 caaaagcccc gtggtgtcgg gtgttccggc atcgatgcaa ggcgatccgg catcaagtga
    10921 aaagggatat tcatcaccca tcgaaaactg cggatcttca ttgatgttgc cggcgttcca
    10981 gtcgagcaca gccccgcctg acttaaagat cccggcttgg gcgctctgga tatctgaata
    11041 cgaaatggtt aaggtatcac tttcgctata atccacaaag tagtatatct ggatcggatt
    11101 attgttatcc cagaggatac tgttggtcag actggctttg cctccataaa tatggagaac
    11161 cggaggtgta ttctgcgtac aggttgcatt gtaaaaattc acttcagcat ctggcggcgc
    11221 accataattg taaatcgtac cggctccgcc ataaccatct gctgccgtat ttctgacaaa
    11281 cagcgtgttt tttgcatcca tcgaggatga ttcgaggcaa aaacctcctc cccggtaagc
    11341 ctggttcgaa tcaaagaccg cattggccag gataaaatct gaatttcgcg catagatagc
    11401 accgccataa ttctctgccg tattcgaaaa acagtaaata tgatccatgg ttaacgtatc
    11461 cggataagat atgtaaatac ctccgccatc agtcgcttga ttatgggtaa aatcgatgtt
    11521 cttcaaatga gccgaggagt atgaaatatc aattccgcct cccgttccgc tttcattatt
    11581 ctcaaaaacc acatgattta attcagcgat gctcccactt ccgacatata caccgccacc
    11641 ctggtagttg cttgtattgt ccctgatctt gacgttgaac aggttaaaca cagaacggta
    11701 acccaagagg cctcctccat tcatgccatg acctccggtc agagttaagt ttttaatttc
    11761 agggatatga tcatgataaa ggttaaggac atgcgatgaa tcttctgcat cgagaatggt
    11821 cccatctctc gtttcgcctt ccagactgca ataatcggat aactgcaaag ggaacttctc
    11881 cccgtttgat gatccgctgt agataccatc tgcaaggtga atgctgtgct gattaaggct
    11941 gtcagatctc aatatcatca gggcgcggtc gatggtcttc agggggttat cagatgaaaa
    12001 tccgctgttg gtatcacttc cgacggtgga tacataaaga tctgcataag cctgttccac
    12061 ttttccggca agaatatcaa aagtaaaatc atccggatca ttcacaaaat agtcggtggg
    12121 atgcaagact gtgaacgtgt caagaatcac attcaccatc ccgtcttttg taaaaatatc
    12181 ttttgcggtg ccggcccagt tcaggaataa attacagcgg tctgtgctgt cgaattgtgg
    12241 aaatgcttcg tcatcacaat aaattcctcc gccataatta tcagcatggt tataagttat
    12301 atccgatttt ttgatatgca gaccggttgt ggcagtaaag atgccgccgg ctgtgttatt
    12361 agataggtta ctattgattt tgacatggtt gaggtaaacc actgtttctg tgccgcaaca
    12421 caggcctcct ccgcccccga tagcatggtt tccggataat tcagatgaat ccagaagagc
    12481 ataggaattg gcatacacat aaagtccgcc tccggaaccg gatgtattat tgacaaggtt
    12541 agagttgaaa atccagatcg aatctctgga ataaatccca cctccggcat taccggatac
    12601 attagcattg ataatgctgt tacgaagttc aaggaatcca taatttaaaa tcccggcacc
    12661 attggaagca tgattttcaa ggacggacaa ttgatcacat atagttcttt cagtggatgt
    12721 cgataaatat aatccgccgc cgctgccggc agtattattt aaaatacaga tgttctgcaa
    12781 cactggcgat gaaccagtaa agtacagacc gccgccacta ctttgagcat aattgccggc
    12841 aatgattata tctttcagaa tggcttccga atgatcaccg gatattcccc ctccgctttt
    12901 agaataatta ccaacgctgg atgaattgtc gcggataata agccgttcca ggcaaggatc
    12961 agagtaataa atgtaaatcc ccgctccaga ttcagccaaa ccgccacaca aagtaagatc
    13021 agaaacatag ttggtatcgt tatcccttat ttgcatgacg cggtctgtac tgtcggcatc
    13081 caggatcacc aggctgtcgg cggttcctga aagatgaacg taatcgggaa gagaaacagg
    13141 gaagaactcc ccgttggttt cgggactata gatgccgtcg aggagatgga tctgccgctg
    13201 gccggattcg gatgcaagaa ggatggtcag ggcatgatga atggttctca gcggatcatc
    13261 ggcagtcagt ccgctgttgt tattatctcc ctccggcgag atatacaggt catattcaac
    13321 ctgttccagc ttgctgttca gtatatcgaa ggtaaattct tctatctccg aagcatgaaa
    13381 ttccgtagga ttcattaccg tgaaagtatc gacgataaca tccacatttg catgagaata
    13441 aagatcattt ccaaccgcag cagagttctg gtatatgttg catcggtgtt ccggattgaa
    13501 tacgacattg ccataatagc cgaacaaaat accaccgccg gtgccggctg tgttgtgaca
    13561 tacggtaatt ccattgaaat atccaccgat ataaaaatcc atcgcaattc ctcctccgta
    13621 acccgcatta ttgtccttta tgaccaggtc ggtcagttgc ggagaatccc acgaatagga
    13681 atccgtccct aaccaaatac ctccaccctg ctctgtcgca gtattcccct ggataaggca
    13741 gttggaaatg gcaggctgac accagtatgc atagataccg cctccgcttg aagcgctatt
    13801 cccataaata ttcagatgat ccaggtatgg atccgattca aagatcctta tacctcctcc
    13861 actgtttccc tttccattgg taattgaaaa gcctgatatc ctggtagatg catcttcacc
    13921 ctggtatatg gt
//
LOCUS       DAOPNH010000049        13799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4501_length_13799_cov_7.321886, whole genome shotgun sequence.
ACCESSION   DAOPNH010000049 DAOPNH010000000
VERSION     DAOPNH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_13799_cov_7.321886"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1527)
                     /locus_tag="PKZ74_04065"
     CDS             complement(106..1527)
                     /locus_tag="PKZ74_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM91963.1"
                     /translation="MDNKAFRESAHRFADWMADYIEQAEQYPVKAKTAPGEIFRLLQD
                     DVPVKGEEMEDIFRDFENIILPGMTHWQSPNFFAYFPANSSYPSVLAEMLTATLGAQC
                     MKWETSPAAAELEEKMMEWLKKICGIPMDFQGVIQDSASTATLVAILCAREKFSGFTV
                     NTEGMQGAGDFRIYCSTETHSSIEKAVRIAGMGSRNLVKVAVDEQFRLDPAQLQQAIE
                     DDLASGKKPICVVATLGTTGVTAIDPLRVIGELCARYGIWLHVDAAYAGSALILPEYR
                     WMAEGLEMADSFVFNPHKWLFTNFDCSAYFVRDKGTLLRTLQIVPEYLRTGDAGRVND
                     YCDWSVPLGRRFRALKLWFVLRSFGLSGLQEKLRGHLRMAGDLAEIIRQEKDFEIMAP
                     VTLNLVCFRYKPEGVEDEGELNLINEKMLRLINSTGEAYMTHTKINGKYAIRMVVAQT
                     NVTMEHVIKAWGLVQSSKIKDQS"
     gene            1645..2022
                     /locus_tag="PKZ74_04070"
     CDS             1645..2022
                     /locus_tag="PKZ74_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91964.1"
                     /translation="MDTRKFEYLETIRETIETGDYEELDDYIEDIRDQVKADPEFQEL
                     FGEITSRNYGDAISLIDEFIYQDIQSGQDDFFNEESLNRLGGLDYPKNLEGFTEEGVP
                     EEISFEEFIEDDFVGGAGQEEEY"
     gene            2044..3714
                     /locus_tag="PKZ74_04075"
     CDS             2044..3714
                     /locus_tag="PKZ74_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91965.1"
                     /translation="MKKFFWISALFLLSAGIALLILARYAEHVAEPYILTFLEHNKPG
                     HHKIEYGKIRLNLLNSTVNIRDVRVFPEPEYSGNENVWMVLNVGLIKLKRIDLWELFF
                     HKNLYISDFIILRPETRLHLPDRITSQSVENVTAEEDVQVKPQLLKSIALDKMIISGG
                     TFRLYRNEDIIASSDNIGFLARNISLVKNTDDDPLGYSYGEVKFILTDLWFQSETGLY
                     DMGLDKFTVVKKDSSVILEGFRMKPKYGREEFSKQLKSQDDRFDIDIGKIEVAGIGYR
                     RFLEGNPLEITRIMIDGMTADIYRDKNVPFDLMNYPEYYNELFLKLSIPLQINAVEID
                     NSRLIYSELATGAAQAGEIIFEDFKLVSYKLNNRPVNDSVEDFIQINVKAKIMGEGPL
                     NADLVLPLEGNTRAIRCSGSVGAMKVVPLNGMLEPSINIKFNDGLLTRMTFAFEGDER
                     LSNGWMEFLYKDLNVAILKKEPGKEWGFLSMLANTVTLSNNPHSGKDPKIVAIGYERD
                     RNKGVINFIWKTIQSGMIRTIIPVNKYQINKKQKEVRVPEGKASKKKK"
     gene            complement(3735..4178)
                     /locus_tag="PKZ74_04080"
     CDS             complement(3735..4178)
                     /locus_tag="PKZ74_04080"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPM91966.1"
                     /translation="MGRKIKLTGGSSYDYTTIGIACHMKDYRFAFFMNESLGFHFKRL
                     DDLISGEEAESRGYSFYLYSHPEERRDYYLVSNYHEDGRLIPSEKGADFFLIVNDILP
                     AALKKEITGKIQKVPQALAAYEIPKGKIKNLEIIFLEIEEHLLPG"
     gene            complement(4178..6202)
                     /locus_tag="PKZ74_04085"
     CDS             complement(4178..6202)
                     /locus_tag="PKZ74_04085"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPM91967.1"
                     /translation="MSFRVNDMRIGILGFAPLILCFALLFSSCSSDEAVRLDSPDGRN
                     NIVFILREGKPFYRVIRDTRVVIDTSALGFEIRDGLPIGENLRMEKATMSGEQNRWEP
                     AWGQRNRIREHYGQLQVILKEKTGPGRTVQLEFRAYNDGVAFRYVVPEQEGLAKLEIM
                     KELTEFRFRDDLSAWWIPGDYDNHEFLYRNTPISGIDSANTPLTMESLTDSLCLSLHE
                     AALVNYPEMRLKRSADAPLTLLCDLVPWPDGVKARVEVPFQTPWRTVQLSKTAGELIE
                     SSLILNLNEPTKLEDISWIRPMKYIGIWWGMHIGKYTWTAGPKHGATTENAKRHIDFA
                     SENNIPGVLVEGWNKGWENWADGVEFPMMEAYDDYNLAEVAGYAKNKGVYLIVHNETG
                     ANAEFYETQVDSAFALYERLGVHAIKTGYVGKMRPEGQYHHGQWMVNHQAMIIEKAAK
                     HKIMMDAHETVKATGLERTWPNFMSREWGRGNEYEAWSEGNPPDHTCILPFTRVLAGP
                     FDFTPGITGIYFKDYKPDNRIHTTLAKQLALMVVIYSPLQMAADLIENYEGHPAFRFI
                     REVPVDWDQTKALNGKIGDYVTIARKKNNAWYIGSITDENPRELLVKLDFLDEKVMYE
                     AVVYADGPDADWVSNPLDYVIERRMVKKDDEYVIRLAPGGGQAITVTPVN"
     gene            complement(6276..7916)
                     /locus_tag="PKZ74_04090"
     CDS             complement(6276..7916)
                     /locus_tag="PKZ74_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPM91968.1"
                     /translation="MKNNTPFHLMILLCVFCAFLLPSLLNAYPGQVVKSFDLPGKYCT
                     GITWDGKNIWVSDRKADLLYCIDPDNGKVIRTLPSPGYWPLALAWDGKNLWNADLKGG
                     TDISENYDGIIYRLDPATGHILQTLDAPSASPIGLAYDGRYLWCADDLSDELIRFSPD
                     DGTTITSFKSPASFSTGLAFDGTYLWVCDRSKDEIYMVSPETGRVVLITPSPGPYPAG
                     LCYDGEYLWHIDYQTDKLYKIVARDDEKFLRSKERKGKLIFTHQIKNFGPGKVTDADL
                     HIAIPVDRDNQQFTAAPSYNMKPAEIVTDRWGQKTARFHFEDLGAGETRTVEMTTLAT
                     TWEVRYFIFPDKVGSRGDIPAEIRGKYLEDNEKFQITHPVIRDAVAAVVGDQTNPYWI
                     VRDIFDHINANMYYEMTGGWNTAPTVLARGSGSCSEYTFVFIAMCRAAGIPARYAGSV
                     VVREEDASMDDVFHRWAEVYLPNYGWIPVDPSRGDQSWPRDQANAIGSVSGTLLVTTE
                     SGGGSETLGWTYNSNQFWTTEPKTYVNFENFGDWEIVE"
     gene            complement(8120..8836)
                     /gene="rnc"
                     /locus_tag="PKZ74_04095"
     CDS             complement(8120..8836)
                     /gene="rnc"
                     /locus_tag="PKZ74_04095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPM91969.1"
                     /translation="MVKFVIVQFKKDKILYTAIKNLLGFYPCNIHLYKVALSHKSAPQ
                     VWLKGQQVNNERLEYLGDAVLSSVVADYLYKKFPYQHEGFLTEMRSRIVSRSRFNKLS
                     LKMGLNKLIFQGNGAYTSSKSIYGDTFEALVGAIYLDKGYNFTSKIIIRRIIDVHLDV
                     DEIEKTDTNFKSRILEYAQREKKPLEFKVLQEIGDGHRKQYMVELYIDGLPVSRGLDY
                     SIKAAEQSAAEKACEELLSE"
     gene            complement(8853..10106)
                     /gene="fabF"
                     /locus_tag="PKZ74_04100"
     CDS             complement(8853..10106)
                     /gene="fabF"
                     /locus_tag="PKZ74_04100"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPM91970.1"
                     /translation="MTSRRVVITGLGALTPLGNTVEELWTGLVNGVSGAGPITRFDAS
                     KFKTQFACEVKNLDMGLYFDRKEVRKYDLYAQYALIAADQAVTDSGINSESVDKERVG
                     VIWASGIGGLETFENEIISFAKGDGTPRFNPFFIPKMIADIAAGHISIRYGFMGPNFA
                     TVSACASAANAMVDAFNYIRLGFADAFIVGGSEASITIGGVGGFNSMHAISTRNDDPK
                     TASRPFDKDRDGFVLGEGGCGLIFEEYEHAMARGAKIYAELVGGGLSGDAYHMTAPHP
                     DGKGAMLAMRNALKDAGIRPEEIDHINTHGTSTPVGDIAEPRAIVDTFGEHAYKISIN
                     STKSMTGHLLGAAGAIEAIATILAIRNDIIPPTINHFTDDPEIDNNLDFTYHTAKKRV
                     VNYALSNTFGFGGHNASLIFKKYRG"
     gene            complement(10122..10358)
                     /locus_tag="PKZ74_04105"
     CDS             complement(10122..10358)
                     /locus_tag="PKZ74_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPM91971.1"
                     /translation="MSDIATRVKAIIVDKLGVDENEVTAEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDAAEKIGTVGDAIKYIEENTK"
     gene            10603..11184
                     /gene="purN"
                     /locus_tag="PKZ74_04110"
     CDS             10603..11184
                     /gene="purN"
                     /locus_tag="PKZ74_04110"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPM91972.1"
                     /translation="MINIAIFASGNGSNAQNIAEYFQNHPAIRVSLILANKPDAFVLE
                     RAKNLGIPSHVFNRKEFYETNDVLQVLLDHHTDFIVLAGFLWFVPDNLLSNFSRKIIN
                     IHPALLPRHGGKGMYGDKVHKAVIESGDKVTGITIHFVNDHYDEGEVIFQESIPVIPG
                     DTSESIAQKVHQLEYLHFPRIIEEVIKSSSVGG"
     gene            11317..11424
                     /locus_tag="PKZ74_04115"
     CDS             11317..11424
                     /locus_tag="PKZ74_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPM91973.1"
                     /translation="MESSERIILKSIELFTRYGIRLVTMDQIAEEVGMS"
     gene            11440..11925
                     /locus_tag="PKZ74_04120"
     CDS             11440..11925
                     /locus_tag="PKZ74_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91974.1"
                     /translation="MFKDKDTLVMECLESIHRKQMTEIQEIAERVSNVIEALYRVGQY
                     SEKKKAEMNPIFFEDIRKLYPQIWNTMKQRSNTGSGMFSHMILTKGIGEGIFRDDLDI
                     TIVGTFMQSMMETFHKKEIFPENTPDKALLRNIVIPYYMGISTDKGKKLIEQYIPLTF
                     S"
     gene            11951..13249
                     /locus_tag="PKZ74_04125"
     CDS             11951..13249
                     /locus_tag="PKZ74_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM91975.1"
                     /translation="MRKTALFLLLWAFTGCFQASGQEKLALSLEQAINYALEYNKQLE
                     NAAISVDLAQKKINESISGFLPQADLSMDYSNYLGAEMEFSFGEGLPAQKIPFNPTSN
                     LYLRVSQMVFSGNMIVGLQMVKIYKEMSSTNYLKTEQSLKENTIKAYQNALVSEKYIG
                     IINENLANTRQIYENTKAMVSVGMAEKLDLDQFEVTLSTLENALKSAERQKELAYNLL
                     RFQLGVQADTPVDLTDSLGFFIENFDITGLADKSFNLSNNLDYQVMQSQVQISGKQVS
                     MQKMNYLPTLTGFYQHTNKLMKPEFDLSPKNVIGLNFNFPILSSGMRNSQYNQARLQY
                     ETTLNNKSLLTDQLLLQEKQLRFNLASAIEQYLNRKNNVDVTYRIYKSYRFKYEQGIA
                     SSLDLTIANNNYLQAESDYLMSIMTLLDARTALQKLLNTL"
     gene            13274..>13799
                     /locus_tag="PKZ74_04130"
     CDS             13274..>13799
                     /locus_tag="PKZ74_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPM91976.1"
                     /translation="MKFLRSLPAIVLSVFILAGCGSMEGEKKNESSAASASAIKIEKV
                     KVMALEPVTIGKNIEYTSTLKAFEEVHLVPSTPGRIEKIYVEVGSRVSKGDVLVQMDQ
                     TQLRQAIIQLQTLETDYKRLDTLQKVGSITRQQFDQISAQVEIARNNVEFLKENTRLV
                     APFGGIISGQYFEDG"
BASE COUNT         3603 a         3595 c         3153 g         3448 t
ORIGIN      
        1 ccaatccctg acttcaattt tgaactcagc actttgaact tcaactttga actttaactt
       61 tgaacttcaa ctttgaactt taactttgaa cttcaacttt gaactttaac tttgatcttt
      121 gatctttgaa ctttgaacta atccccacgc ttttatcacg tgttccattg taacattcgt
      181 ttgtgcgacc accatccgga tcgcatattt accgtttatc tttgtatgag tcatgtaagc
      241 ttctcctgtg gaattgatca gccggagcat cttttcattt atcaggttaa gctccccttc
      301 gtcttccacc ccttccggtt tgtaacggaa gcagaccagg ttaagcgtca ccggcgccat
      361 aatttcaaag tctttttcct gacggatgat ctccgccagg tcaccggcca tacgaaggtg
      421 gcccctcagt ttttcctgta accccgacag cccgaagctg cgcagcacaa accacagctt
      481 cagcgcccgg aaacgcctgc ccagcggcac actccagtcg cagtaatcgt tcaccctgcc
      541 cgcatcgccg gtgcgcaggt attcgggtac aatttgcagg gtcctgagca acgtgccctt
      601 atcccggaca aagtaagccg aacaatcaaa attggtgaac aaccatttgt ggggattgaa
      661 gacaaaactg tcggccatct ccagcccctc cgccatccag cggtattccg gcaggatgag
      721 cgccgaaccg gcataagcgg catcgacatg cagccagatt ccatacctgg cgcacaattc
      781 gccaatgacc cgcaacggat cgatagccgt tactcctgtg gtgcccaatg tcgctactac
      841 gcatattggc ttctttcctg atgccaggtc atcctcaatg gcctgctgaa gctgcgccgg
      901 atcaaggcga aactgctcat ctacagcaac tttaaccagg ttccggctgc ccatcccggc
      961 aatcctcaca gccttttcaa tgctcgaatg agtctcagtg gagcaataga tccggaaatc
     1021 acctgcgccc tgcatgccct ccgtatttac agtaaatccg gaaaacttct cccgggcgca
     1081 gagaatagcc accagggttg ccgtcgatgc cgaatcctgg ataaccccct gaaaatccat
     1141 cgggatgccg catatcttct tcagccactc catcatcttt tcctccagtt cggcggcggc
     1201 cggcgaagtc tcccatttca tgcactgcgc gccgagtgtg gcggtcagca tctccgccag
     1261 caccgaagga taactggaat tggccggaaa ataagcaaag aaattcgggc tctgccagtg
     1321 cgtcatcccc ggaaggataa tattttcaaa atccctgaaa atatcttcca tctcctcccc
     1381 cttaactgga acgtcgtctt gtaatagtcg gaatatttca cccggcgcgg ttttcgcttt
     1441 tacaggatac tgttcagcct gttctatata atcggccatc cagtcggcaa accggtgggc
     1501 actctcacga aatgctttgt tatccatgtc gttccgtttt acgtacaaag atcatcatgt
     1561 ttttttcaat caacccgctc tttttagcaa aaatatgctt acatttaaac tgcatttgat
     1621 aatggataac taatttttgc tgcaatggat accagaaaat tcgagtacct ggaaacaatc
     1681 cgtgaaacca ttgaaacggg tgattatgaa gaactggatg attatattga agatatccgc
     1741 gaccaggtaa aagccgatcc cgaattccag gaactcttcg gggagatcac aagccgtaat
     1801 tacggcgatg ccatttcttt gatcgatgag tttatctacc aggatatcca gtcgggccag
     1861 gacgattttt ttaatgaaga atcgctgaac aggcttggcg gactggatta tccaaagaat
     1921 ttggagggct ttaccgaaga aggagtgccg gaagaaattt cgtttgaaga gtttattgaa
     1981 gatgattttg tcggaggtgc aggccaggag gaagagtatt aatatctggc cgttcgacgc
     2041 acgatgaaga aattcttctg gatttcagct ttattcctcc tttctgccgg gattgctctc
     2101 ctgatcctgg cccgctatgc ggaacatgtc gccgaaccgt atatccttac cttccttgaa
     2161 cacaacaaac cgggccacca caaaatcgaa tacggtaaga tcaggcttaa cctgctcaac
     2221 agtacggtaa atatccgtga tgtccgggtt ttcccggaac cggaatattc agggaatgaa
     2281 aacgtctgga tggtattgaa tgtcggcctt atcaaactga aacggattga cctttgggaa
     2341 ttatttttcc ataagaacct ctatatcagc gattttatta tactccggcc cgaaacccgt
     2401 cttcatctgc ctgaccggat caccagccag tctgtcgaaa atgtaactgc cgaagaagat
     2461 gtgcaggtaa aaccgcagct gctcaaaagt atagcgctcg ataaaatgat catctccggc
     2521 ggtactttcc gcctttaccg caacgaagat attatcgcca gttccgacaa tattggcttt
     2581 ttagcacgga atatcagcct ggttaaaaat actgatgatg acccgctggg ctattcttac
     2641 ggagaagtga agttcatact gaccgatctg tggttccagt ctgaaaccgg cctctatgac
     2701 atgggcctgg ataaattcac cgtcgttaaa aaagattcat ccgttatcct tgaaggattc
     2761 cggatgaaac ccaaatacgg cagggaggaa ttctcaaaac aactgaaatc acaggatgac
     2821 cggttcgata ttgacatcgg gaagatagag gtggccggga tcggttaccg cagattcctc
     2881 gaagggaacc cgctggaaat tacccggatc atgatagacg gaatgaccgc agatatttat
     2941 cgcgataaaa atgttccgtt cgatttgatg aactacccgg aatattacaa cgagctattc
     3001 ctgaaattaa gcatccctct tcagatcaat gctgttgaga tcgacaattc ccggctgatt
     3061 tacagcgaac tggcaaccgg tgccgcccag gctggcgaaa ttattttcga agactttaaa
     3121 ctggtttcgt acaaactgaa taacaggcct gtcaacgact ctgtcgagga tttcattcaa
     3181 ataaacgtaa aggctaaaat catgggagaa ggtccgctga atgccgacct ggttttgccg
     3241 ctggaaggga acacaagggc aatcaggtgc tctggcagcg tgggggctat gaaggtcgtg
     3301 ccactgaacg gtatgctgga accttccatc aatataaaat tcaacgatgg cctgcttacc
     3361 cggatgactt tcgcttttga aggagatgaa aggctttcaa acggctggat ggaatttctc
     3421 tataaagatc ttaatgtggc cattttgaaa aaagaacccg gcaaagagtg gggattcctc
     3481 tccatgcttg caaatacggt cacactctcc aacaatcccc attccggaaa agatccaaaa
     3541 atagtagcta ttggttatga acgcgaccgg aataagggtg tgatcaattt catctggaaa
     3601 acaattcaaa gcggaatgat ccggactata atcccggtta ataaatacca gatcaacaaa
     3661 aaacagaaag aggtgcgggt accggagggt aaggcaagta agaaaaagaa ataaattcgg
     3721 aatgaaccgg cagcttatcc cggcaacaaa tgttcctcga tctccaggaa aataatctcc
     3781 aggtttttga tctttccctt tggaatttca taggcagcca gtgcctgcgg taccttctgg
     3841 attttcccgg tgatctcctt ttttaaagcc gcaggcaaaa tatcgttcac gatcaggaaa
     3901 aaatcggccc ccttctcaga agggatcaaa cgcccatcct catgataatt ggaaaccagg
     3961 taataatccc gcctctcttc cggatgactg tacagataaa aggaataacc gcgcgattcg
     4021 gcttcctcgc ccgatatcag gtcatccagc cttttaaaat gaaaacccag gctttcattc
     4081 ataaagaagg cgaaccggta gtccttcata tggcaggcga tccctatcgt tgtgtaatcg
     4141 taggagctgc caccggtgag tttgatcttc ctgcccatca gtttacaggt gttaccgtga
     4201 tggcctgccc gcctcccggg gccagccgga tcacatattc atcgtccttt ttcaccatcc
     4261 tcctttcaat gacgtaatcc agagggttgg aaacccaatc ggcgtccgga ccgtcagcat
     4321 agaccacggc ttcatacatc accttttcat ccaggaagtc cagtttgaca agcaactcac
     4381 gcggattttc atcggtgatc gacccgatgt accaggcatt gttctttttc cgggcaatgg
     4441 tcacataatc gccgatcttc ccgttaagcg ccttcgtctg gtcccagtca accggcactt
     4501 cccggatgaa ccggaacgcc ggatgacctt cgtaattttc gatcagatcg gccgccatct
     4561 gcaacgggct gtaaataaca accatcaatg ccagttgctt cgccagggtg gtatgaatcc
     4621 ggttatcggg cttataatct ttaaaataaa tgccggttat gccgggtgta aaatcgaacg
     4681 gcccggccag gacccgtgtg aacggcagga tacaggtgtg gtccggggga ttgccttcgc
     4741 tccacgcttc gtattcgtta ccccgtcccc attcgcggct catgaaattc ggccaggtgc
     4801 gttccagccc ggtcgccttc accgtttcat gcgcatccat catgatcttg tgcttcgccg
     4861 ctttttcaat gatcatcgcc tggtgattga ccatccactg cccgtgatgg tattgcccct
     4921 caggcctcat cttacctaca tagccggttt ttatggcatg gaccccaagc cgctcataaa
     4981 gagcgaaagc ggaatccacc tgggtctcat aaaattcagc gttggcgccg gtttcattat
     5041 ggacaatcag gtaaacgccc ttattttttg catatcccgc cacttccgca agattgtaat
     5101 catcataggc ttccatcatc ggaaactcaa cgccgtcggc ccagttctcc catcctttgt
     5161 tccaaccctc taccagcacc ccgggtatgt tgttctctga cgcaaaatcg atgtgccgct
     5221 tcgcattctc cgtggtagcc ccatgcttcg ggccggctgt ccaggtatac tttccgatat
     5281 gcatccccca ccagatgccg atgtacttca tcggcctgat ccagcttatg tcttccagct
     5341 tcgtgggttc attcaggttc aggatcaacg atgattcgat cagttcgccg gcagtttttg
     5401 aaagttgcac cgttctccag ggcgtctgaa aagggacttc aaccctggct tttaccccgt
     5461 cgggccatgg caccaggtcg cacaacagcg ttaaaggagc atcggcagac cttttcagcc
     5521 tcatttcagg gtaattcacc agcgccgctt cgtgcaggct caggcacagg ctgtcggtca
     5581 gcgattccat cgtcagcggg gtattggccg aatcaattcc gctgatgggg gtattgcggt
     5641 aaagaaattc atgattgtca taatccccag gtatccacca ggcgctgaga tcatccctga
     5701 agcggaattc ggtcagctcc ttcatgatct ccagtttggc cagcccttcc tgctcgggaa
     5761 cgacataccg gaaagcgacg ccgtcgttgt acgccctgaa ttcaagctgc actgtccggc
     5821 cgggtccggt cttctctttc aggatcacct gaagctgccc gtaatgctcc ctgatccggt
     5881 tgcgctgccc ccaggccggt tcccagcggt tctgttcacc cgacatggtc gctttctcca
     5941 tcctgaggtt ttccccgatc ggcagtccgt cccgtatttc aaaacccagc gccgatgtgt
     6001 cgatcacaac ccgggtatca cggatcaccc ggtaaaaggg ctttccctcc ctgaggatga
     6061 aaacaatgtt atttcttcca tccggtgaat caagcctcac cgcctcatcc gaagaacagg
     6121 atgagaaaag taaagcaaaa cacaaaatca atggcgcaaa tcctaaaatc ccaattctca
     6181 tatcattaac tctaaaactc ataactaaaa ttcgtaaccc gaaacccgaa acctgaaacc
     6241 tgaaacctga aacctggaac ctggaacttg gactatcact caactatctc ccaatccccg
     6301 aaattctcaa aattcacata ggtcttcggt tcagtggtcc agaactggtt ggagttgtag
     6361 gtccagccca gggtttccga tccgcctcca ctctcggttg tcaccaggag cgtaccgctg
     6421 acactgccga tggcattggc ctggtcgcgg ggccagcttt ggtcgccgcg gctcggatct
     6481 accgggatcc agccgtagtt cggcaggtaa acctccgccc agcgatgaaa gacatcgtcc
     6541 atgctggcat cctcctccct gacaaccacc gaaccggcat acctggccgg aatgccggct
     6601 gcccggcaca tggcgatgaa aacaaaggtg tactccgaac acgagccgct gcccctggcg
     6661 agaactgtcg gtgccgtatt ccaccctccc gtcatttcat aatacatgtt ggcattgata
     6721 tgatcgaaga tatcccgtac gatccagtat ggattggtct ggtctccaac aactgccgca
     6781 acggcatccc ggatcaccgg gtgggtgatc tgaaacttct cgttgtcttc caggtactta
     6841 cctctaattt cagccgggat gtctcccctg gaaccaacct tgtcgggaaa aataaaataa
     6901 cgcacctccc aggtggtggc cagcgtcgtc atttcaaccg tccgggtctc gccggcgccc
     6961 aggtcttcga aatgaaaccg ggccgttttt tgtccccagc ggtcggtgac gatctccgct
     7021 ggtttcatat tgtaagatgg tgccgcggta aactgctggt tatcacggtc gaccgggatg
     7081 gcaatgtgca ggtccgcatc ggtcaccttt cccgggccaa agttcttgat ctggtgcgta
     7141 aagatcaatt tgccttttcg ctccttcgaa cgaaggaatt tttcatcgtc cctggcgacg
     7201 atcttataca gtttatcggt ctggtaatca atatgccaga gatattcccc gtcgtagcag
     7261 aggcctgccg gataaggccc cggcgatggg gtgatcagaa ccaccctccc tgtctccggg
     7321 ctgaccatgt aaatctcgtc tttcgaccgg tcgcaaaccc agaggtaagt gccatcaaag
     7381 gccaggcccg tagagaatga agccggggat ttgaaggagg tgatcgtggt tccgtcgtcg
     7441 ggactgaacc ggatcagttc gtcggaaagg tcgtcggcac accataggta ccggccgtcg
     7501 taagcaagcc cgatggggct ggccgaagga gcgtcgaggg tttgaagaat atgtccggta
     7561 gccggatcaa ggcggtagat gatgccatcg taattctccg agatatccgt tccccccttc
     7621 agatcggcgt tccagaggtt cttgccgtcc caggccagtg ccagtggcca gtagcccggc
     7681 gatggcaggg tgcggatcac tttgccattg tccgggtcga tgcaatacag caggtcggcc
     7741 ttccggtcgg agacccagat gttctttccg tcccaggtga taccggtaca atatttcccc
     7801 ggcaggtcga atgatttgac cacctggccg ggatatgcat taagaagaga aggcagtaaa
     7861 aaagcacaga aaacacataa taagatcatt agatgaaagg gagtattatt tttcatgact
     7921 aagaatttat ttcgaggatg aaattatgga aaataataaa acgaggaggc tgtctccaaa
     7981 ggctttttat taaccgcgaa ggcgcacaaa gtacatcaca aaggaacact aagtagttga
     8041 atatcaattt acataccttt gtgcgccttt gtgaattcct tggtgtcctt tgtggttaaa
     8101 tccttttgag acatcctcct cactcggaaa gtaattcctc gcaggctttc tctgccgccg
     8161 attgctccgc cgccttgatc gaataatcca ggccccgcga aaccggcaac ccgtcgatat
     8221 aaagctctac catgtattgc ttccggtgac catccccaat ctcctgcaat accttgaatt
     8281 cgagcggctt cttctcccgc tgggcatatt ccagtatccg gctcttgaaa ttggtgtcgg
     8341 tcttctcaat ttcatccaca tccaggtgca cgtcaatgat cctacggata atgatcttgc
     8401 tggtgaaatt atagcctttg tcgagataaa tggcaccgac cagcgcttca aacgtatcgc
     8461 cgtaaatcga cttgctcgaa gtatacgccc cgttgccctg gaatatcagc ttgttcagcc
     8521 ccatcttcag ggagagtttg ttgaaccggc tgcggctcac aatgcgcgaa cgcatctcgg
     8581 tcagaaaacc ttcatgctgg taaggaaatt tcttgtaaag ataatcggcc acgaccgaac
     8641 tgagcacggc atcgcccagg tattcaagcc tttcgttgtt tacctgctgg cctttcagcc
     8701 acacctgcgg agccgattta tgggaaaggg ctactttata aagatgtatg ttgcagggat
     8761 agaatcccag caggttttta atcgctgtat aaaggatttt gtctttttta aactggacaa
     8821 taacaaattt taccatcacc ctgccgggaa ggttatcccc ggtacttttt gaagataagg
     8881 gaggcgttat gcccgccaaa cccgaaggta ttgctcaggg cgtagttaac aacccgcttt
     8941 ttggctgtat gataagtaaa gtcaaggtta ttgtcaatct ccggatcatc ggtgaaatgg
     9001 ttgatcgtcg gaggaatgat atcattccgg atagccagga tggtggcaat agcttcgatg
     9061 gcgccggcag cgcccagcag gtgaccggtc atcgacttgg ttgaattgat gctgatctta
     9121 taggcatgct ccccgaaggt atctacgatg gccctgggtt cggcaatgtc gccaacgggc
     9181 gtcgatgtgc cgtgcgtatt gatgtgatcg atctcttccg gccggatgcc tgcatctttc
     9241 agggcatttc tcatggccag catcgcccct tttccgtcag gatgcggagc ggtcatgtgg
     9301 taagcgtcgc ccgacaggcc tccgcccact aattctgcat agatttttgc tcccctggcc
     9361 atggcatgtt cgtactcttc aaaaataagg ccgcatccgc cttcacccag gacaaacccg
     9421 tcgcggtctt tgtcgaatgg cctcgacgcc gttttcggat catcattccg ggtggagatg
     9481 gcatgcatgg aattaaaccc gccaacgcca ccgattgtaa tggatgcttc agagccaccg
     9541 acgataaaag catcggcaaa tcccagtcgg atgtagttaa aagcatccac catggcattg
     9601 gctgccgagg cacaggcgga gaccgtcgca aaattcggcc ccatgaatcc gtaccggatc
     9661 gaaatatgac cggccgcgat atcggcaatc attttgggga tgaaaaacgg attgaaccgg
     9721 ggagtgccgt ctcctttggc gaagctgatg atctcattct cgaaggtttc caaccctccg
     9781 atcccggatg cccagatgac gccaacccgc tctttgtcaa ccgactccga attgatcccc
     9841 gagtcggtaa cggcctggtc ggctgctata agggcatatt gtgcataaag atcgtatttc
     9901 ctgacttcct tgcggtcaaa atacaggccc atgtcgaggt tcttcacctc acaggcaaac
     9961 tgggtcttga atttcgacgc atcaaaacgc gtaataggcc cagcgccact aaccccattg
    10021 accaatccgg tccacaattc ctcaaccgta ttgcccaatg gggtaagggc tccaagtccg
    10081 gtaatgacta ctcggcgtga agtcatgaaa taaatttaga cttattttgt gttctcttcg
    10141 atgtatttta tcgcatcacc cacggttccg atcttctcag cggcatcatc cgggatggca
    10201 atgttgaatt ccttctcgaa ttccatgatg agttcaaccg tatcgagcga atcagcgcct
    10261 aaatcgttag tgaagctggc ttctgcggta acttcattct catctacacc aagcttatca
    10321 actatgatag cttttactct ggttgcaatg tctgacatac ttaaaatgat tttggttaaa
    10381 caagctgcaa agaaaataat aatcttgaca taatacaaga ggaagataaa atattttggg
    10441 gccggaatgt tatatgcaat tcattatcaa ttatatatta ttttctggat attttatttg
    10501 ccaggtttat catacatctt atattttaaa aatcaggaaa ttaaatccgg atagccaaat
    10561 ggaccggaaa tctgtatatt tgaaccggat tttccctgac atatgataaa catcgccatc
    10621 tttgcctcag gaaacggcag caatgcccag aatatagctg agtactttca gaaccatcct
    10681 gccatccggg tctcgctcat cctggctaac aaacccgatg cctttgtcct cgaacgggca
    10741 aaaaacctcg gaatcccatc gcatgtgttt aaccggaaag agttttacga aacaaacgac
    10801 gttctgcagg tccttctcga tcatcatacc gacttcatcg tgctggccgg atttctctgg
    10861 ttcgtgccgg ataatctcct gagtaatttc tcccgtaaga tcatcaatat ccatcctgcc
    10921 ctcctgcccc gccacggcgg aaaaggcatg tacggcgaca aggttcataa agccgtgatc
    10981 gaatcaggcg ataaagtaac gggcatcact atccattttg tgaatgatca ttacgatgaa
    11041 ggagaggtca ttttccagga atcgatcccg gtaatccctg gtgatacgtc cgaaagcatc
    11101 gcccaaaaag tccatcaact cgaatacctg cactttccaa ggattattga agaggtcatt
    11161 aaatcgtcat cggttggcgg ttgatgtttc aggggaaatc ccgttatgtc acaaagtctt
    11221 taatcgttga tcccggtctt atttttcagc acaaaaaaaa atgaaaactg aataaaccga
    11281 tcaagtttta ctatttttgc cgctcatttt gttatgatgg aatcgagcga aagaattatc
    11341 ctgaaatcca ttgaactgtt tacccgatac ggcatccggc tggtgacgat ggaccagatc
    11401 gccgaagagg tgggtatgtc gtaacggact atctatgaat tgtttaaaga caaagacacc
    11461 ctggtcatgg aatgccttga atcgatccac cggaaacaaa tgaccgaaat acaggaaatt
    11521 gccgaaagag tttcaaacgt catcgaggct ttataccgcg tcgggcaata tagcgagaaa
    11581 aagaaagcag aaatgaatcc catttttttt gaagatatcc ggaaactata tcctcaaata
    11641 tggaatacca tgaaacaacg ctccaatacc gggtccggta tgttctccca tatgatcctg
    11701 acaaaaggga ttggggaagg cattttccgg gatgacctgg atattacaat tgtcggtaca
    11761 ttcatgcaat ccatgatgga gacttttcac aaaaaagaga tattccccga aaacacccct
    11821 gacaaagccc tgctccgcaa tatagtcatc ccttactata tgggtatctc aacggataaa
    11881 gggaagaaac tcatcgaaca atatatacca ttaacatttt cttgatcact actaataatt
    11941 atattcagca atgcgtaaaa cagcgttatt tcttttatta tgggcgttta caggctgttt
    12001 tcaggcatca ggacaggaaa aactggcact atccctggaa caggcaataa attatgcact
    12061 cgaatacaac aagcaactcg agaatgccgc tatctccgtc gacctggccc agaaaaaaat
    12121 aaacgaatcg atttccggtt tcctccccca ggccgatctg tctatggatt actcgaacta
    12181 tctcggcgcc gaaatggagt tcagcttcgg ggaaggcctc ccggcacaga aaattccgtt
    12241 taaccctacc agcaacctgt atctcagggt atcccaaatg gtcttcagcg gaaatatgat
    12301 cgtcgggctt caaatggtga agatctataa ggagatgtcg tctacaaatt acctgaaaac
    12361 ggaacaaagc ctgaaggaaa acaccatcaa agcctaccag aatgccctgg tatcggaaaa
    12421 atatatcggc attatcaatg aaaacctggc caatacccgc cagatctacg aaaacaccaa
    12481 agcgatggtc tcggtgggta tggcggaaaa actcgatctc gaccagttcg aggtcaccct
    12541 ttccacactg gaaaatgccc tgaaatcggc cgaaaggcag aaagaactgg cctacaacct
    12601 gctccgcttc cagctgggcg tgcaggccga tactcccgtc gatctgacag attctcttgg
    12661 cttcttcatc gaaaacttcg atatcacagg actggccgac aaatcgttca atttgtcgaa
    12721 taaccttgat taccaggtca tgcagagcca ggtacagatc tccggaaagc aggtcagtat
    12781 gcaaaagatg aattacctgc cgacactgac cgggttttac cagcatacca acaagctgat
    12841 gaaaccggaa ttcgatttgt ctccgaaaaa tgtaatcggc ctgaacttta atttcccgat
    12901 cctgtcgagc ggtatgcgca attcgcagta taaccaggcc cgcctccagt acgagacaac
    12961 ccttaacaac aaaagcctgc tgaccgacca gctgctcctc caggaaaaac aactgcgatt
    13021 caacctggcc agcgccatcg aacagtatct caaccggaaa aacaatgtgg atgttacata
    13081 ccgcatttat aaaagctacc ggttcaagta cgagcaggga atagcctcaa gccttgacct
    13141 gacgatcgcc aataacaatt atctgcaggc agaatcggat tacctcatgt cgatcatgac
    13201 cttgctcgac gcacgtaccg ccctgcaaaa acttttgaac acactttaat cgtaaccata
    13261 aaaatcaagt tttatgaaat ttttaagatc acttccggca attgtactat ccgtattcat
    13321 ccttgcagga tgcggatcca tggaaggaga gaaaaaaaat gaatcatcgg ccgcttcagc
    13381 ctccgctatt aaaatagaga aagttaaggt tatggcgctg gaaccggtaa cgatcgggaa
    13441 aaacatcgaa tatacctcaa cgctgaaagc cttcgaagag gtgcacctgg tgccttccac
    13501 cccgggccgg attgagaaga tttatgtcga agtcggaagc cgtgtcagta aaggggatgt
    13561 gctggtgcaa atggaccaaa cgcagctgag gcaggcgatc atccaattgc agacactgga
    13621 aaccgattac aaacggctcg atacgctgca gaaagtaggc agtatcaccc gccagcaatt
    13681 cgaccagatc agcgcccagg tagagattgc ccgcaacaat gtggaatttc tcaaggaaaa
    13741 tacccggctc gtggctcctt tcgggggcat tatctccggt caatatttcg aagatggcg
//
LOCUS       DAOPNH010000050        13766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4520_length_13766_cov_6.579170, whole genome shotgun sequence.
ACCESSION   DAOPNH010000050 DAOPNH010000000
VERSION     DAOPNH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_13766_cov_6.579170"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /gene="rpe"
                     /locus_tag="PKZ74_04135"
     CDS             complement(<1..528)
                     /gene="rpe"
                     /locus_tag="PKZ74_04135"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPM91977.1"
                     /translation="MINFSENTFPLIAPSMLSADFGNLQRDIGMVNQSEADWFHIDVM
                     DGVFVPNISFGFPVLRHIQKHARKPLDVHLMIVDPDRYIEKFRDHGAEVISVHYEACT
                     HLHRTVGAIKKLGALAGVVLNPHSPVSLLEEIIPDVDLVMLMSVNPGFGGQSFIEGTL
                     DKIQKLKEMMALKNTT"
     gene            complement(540..1403)
                     /locus_tag="PKZ74_04140"
     CDS             complement(540..1403)
                     /locus_tag="PKZ74_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPM91978.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGRVELITAEEEVELARRIK
                     AGDKDALEKLTKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKAYARLEQQNEREPDSVEIAE
                     MLDIPESEVKESIRNSGRHISMDAPLIQDEENTLYDVLRGDDTNTPEKELMVESLRKE
                     IDRAISTLTSREADVVRLYFGLNSKHPMTLEEIGEKFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKSYLG"
     gene            complement(1473..3608)
                     /locus_tag="PKZ74_04145"
     CDS             complement(1473..3608)
                     /locus_tag="PKZ74_04145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPM91979.1"
                     /translation="MSQAIVKKFKLGDGREVSIETGRLAKQAAGAVVVKMEDTMLLAT
                     VLVAPEPMENADFFPLTVEYKEKYSATGKFPGGFFKREARPSEYEILISRLVDRALRP
                     LFPDNFYNEIQVQVMLISGDKKNLPDTLVALAASSAIMVSNIPFLGPISEARVARING
                     EFEINPPIARMEEADLDLIVAATMDNVVMVEGQMKEVSEAVMLNAIKFAHEAIKQQCQ
                     AQIELAEMVEAAREKMPVPEPVEDKELETAIHEYAYPKFYDIARQGMNNKHQRHDAFS
                     AVKEAFIGTIPEEKREELEPMISRYFEEEERNAVRNCILDDRIRLDGRGLEDIRPIWC
                     EVDYLPAAHGSAIFTRGETQALVTVTLGTKLDEQVLDGAVVEGKSDFMLHYNFPGFST
                     GEVKPIRGTSRREIGHGNLAMRAIKPMIPADDINPYTVRIVSDILESNGSSSMASVCG
                     GTLALMDAGVKIHKPVSGIAMGLITDPKSGKYAVLSDILGDEDHLGDMDFKVTGTVDG
                     ITACQMDIKIEGLSYEIMEQALEQAKRGRHHILGEMLKTIDSPRPDYKPHVPRIEKMT
                     IPKEFIGAVIGPGGKIIQEIQKDTGSTIIIEEVGDVGVIDIFSGNKESIEAAKERIRK
                     IVAIPEVGEIYNGKVKSIVAFGAFVEILPGKEGLLHISEIDWKRVTDIDQYMKVGQML
                     EVKLIGIDNSTGKLKLSRKALIPQPPQKQ"
     gene            complement(3695..3964)
                     /gene="rpsO"
                     /locus_tag="PKZ74_04150"
     CDS             complement(3695..3964)
                     /gene="rpsO"
                     /locus_tag="PKZ74_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPM91980.1"
                     /translation="MYLSSEKKKEFFATHGKSETDTGSPEGQIAMFTHRINYLTEHLK
                     QNKKDNVTRKSLVTLVGKRRSLLDYLKDVEIDRYRAIVKSLGIRK"
     gene            complement(4090..5373)
                     /locus_tag="PKZ74_04155"
     CDS             complement(4090..5373)
                     /locus_tag="PKZ74_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91981.1"
                     /translation="MEKLQKMKFAIEESVKIRQNQCHPCSVSGTQIKTDATNLHGWLR
                     LWPVLVFISLTVNVSSQQSERNWRLNGHLQSLQSFWIPPESDRWQSMTVIGNRFDFRW
                     NPGNTISFHAGARNSLNVGQMVQTFYPYYAKMSVTEDGFFDLTRLWVSDTSYYFYSNI
                     DRANIKIRKGKFEATIGRQRINWAINMVWTPNDIFNTFNYFDFDYIERPGCDAILLEY
                     FMNETSSVQFGFKMDDEQDITSAIMYRFNRWNYDFQAFAGVMTTDIVAGAGWSGDIKG
                     AGFTGEISYFHPRESLEDTSGILVGSVSANYTFRNSLFINASYLFNSDGTTGPAGYGN
                     ALALYADISAKKFTLAKHSVYAGIGYPVTPLIRADISAIYNPNDKSGFAGPSVDISLS
                     NNVTFFIIGQVFWGKDMTEFGDFGSLCYTRLKWNF"
     gene            complement(5339..6025)
                     /locus_tag="PKZ74_04160"
     CDS             complement(5339..6025)
                     /locus_tag="PKZ74_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM91982.1"
                     /translation="MTTIRTINLQKVYDETKIPVNALNGITLEFHEAEFTAIVGPSGS
                     GKTTLLNMLGGLDNPTSGEVHINGTNITGLSSRKLIDFRLHNIGFVFQSFNLIPVLTA
                     RENVEFILQLQGVGRKEREQRAVELLEAVGLADKIHNRPGDLSGGQQQRVSVARALAS
                     KPRFILADEPTANLDSKSTAELLDIMEKMNRQYKTTFLFSTHDSRVMERARRIVTIED
                     GKVAKDEIRN"
     gene            complement(6112..7335)
                     /locus_tag="PKZ74_04165"
     CDS             complement(6112..7335)
                     /locus_tag="PKZ74_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM91983.1"
                     /translation="MIIFIAWRNIWRNKLRSLVVILAVCLGLFGTLLMMAISNGMVEQ
                     KIDASIHNEISHIQVHHPDYLQEPSLSYSIDSANAIARELKAISGVKAVSGKIKAMAM
                     ASTAATAAGVTINGIDPEQENTVTQIHKYIIEGDYFETKSNSAPVVISKKLCEKLKAK
                     LHSKIIVTIQHTSGEIAYGLFRVTGIYKTANNLFDEPNIFVKKEDLAELTGFDTGKVS
                     EFTILLDESELTDPVSDAVKQKLPGLSVMSWKEIEPLLVAMSAMMDQFGYFLLIIILI
                     AMAFGIINTMTMAVLERTRELGMLMAVGMNRRKIFLMIMTETIYLSLVGTFFGIIISA
                     VTIHFTGMSGINFSAWTEGYESLGYSALVYPSLYLDFYVGMTIMVIITAIISSIYPAR
                     KALKLNPASAIREDA"
     gene            complement(7372..8595)
                     /locus_tag="PKZ74_04170"
     CDS             complement(7372..8595)
                     /locus_tag="PKZ74_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM91984.1"
                     /translation="MKEYFKIAWRNLWRNKRRTLLTIASVLFALLLALIVRSMHLGTW
                     NKMIESTIKSTTGYIQVHQEGYWEDKTLDNSFETSSNLEQKYRSHPNVTYIVPKLESF
                     ALLSSGNQTKGAALTGVDPDVEDLVSGLQKKIIAGKYFTTDDKFIILTEGLANYLKLT
                     VGDTVVILGQGYHGVTAAAALPIGGIVKFTQPDMNNLLTYVPLSLAQEIYSAPGRLTS
                     ISFILNDPEDLDRTRNDLAALGPQNLEVMPWKDLLTELVQSFEGDNVSGLFMLGILYL
                     VVGFGILGTILMTTMERKREFGIMTAVGMRRYKLSIIIVIETLIISFIGILSGVILSL
                     PVIYYFHLNPLPLKGEAADAMAEYNMDPVMPFLLEPGFIINQSMVVMIISMLTLLYPL
                     VVINRFKIINAIKGR"
     gene            complement(8597..9367)
                     /locus_tag="PKZ74_04175"
     CDS             complement(8597..9367)
                     /locus_tag="PKZ74_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPM91985.1"
                     /translation="MKQSFHRLYSILTILCTLLAAFPSIAQDLTAREIVKKAHDKANG
                     QTSQGTMKMTIVRPDWSREVAMKSWSKGTDYYLIYITEPVKDRGQVFMKREQDMWNWM
                     PGINRMIKLPPSMMGQSWMGSDFTNDDLVRMNSIIDDFTHTIVGSEMIERFDCHIIEL
                     IPKPEAAVVWGKIKLWISKNDYYELKGEYYDEDLVLVNTMTSSNIKKMGDRTLPSRMV
                     MVPVDKPGNQTILETIDIVFDKPIDESFFSQQNMKNIK"
     gene            complement(9444..10016)
                     /locus_tag="PKZ74_04180"
     CDS             complement(9444..10016)
                     /locus_tag="PKZ74_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM91986.1"
                     /translation="MPRTESQFEEIREQKTALILATALELFANEGYYPTSISKIAEKA
                     GISKGLMYNYFESKEDLILAVIGKGIRELTEHLDPDHDGFLTSEEFELFINGNFRILQ
                     ENLDYWKLYFSTMMQPPVYKLVKAKYLHFLPPLQQIVEGYFERKGVNDPQLEAVFFDS
                     LIDGIFLNYVMSPENFPLDKMKSLIINRFK"
     gene            complement(10143..10652)
                     /locus_tag="PKZ74_04185"
     CDS             complement(10143..10652)
                     /locus_tag="PKZ74_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91987.1"
                     /translation="MSRVLYKEIQRFNQWWIWLIILLLSGFWIWQLVQQIFMGIPFGD
                     NPAPDFVVILLGLFPLGALLLFRFMILETVIDEQGVKYRFKPFQRKPKLIKPEDVKTY
                     EVKKYNPVMEYGGWGVRYGLKGTAYNVKGNMGAQFDLKNGKRFMLGTQNPDALRYALN
                     KLFAKTDRQ"
     gene            complement(10645..11946)
                     /locus_tag="PKZ74_04190"
     CDS             complement(10645..11946)
                     /locus_tag="PKZ74_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPM91988.1"
                     /translation="MKEIEINKDIFESITRYLKDWLIDSGMSENPAKIIADYSGFLLI
                     IAFALIIFYIAKIILVRWIHRLASRSTSNWDDAFVNRKVFKRLAYLIPALIIHRATPY
                     VIPDYPNTMVVVLTLINVYMATVCVIVGSSVLEAAHDIYNSYEISKTRPIKGFIQVGK
                     IFLYVVYAIVIITILFLRSKGFGWIAGLGAFSAVLLLVFKDPILGFAGGIQLATNDML
                     RIGDWIEMPKYNADGIVTDIAITTVKVQNWDKTITTIPTYFLVSDSYKNWRGMQESGV
                     RRLRRHILIDLNSIKFCTPEMLERFSKYEYVSDYITQTEARLNQYNIDKGIDPEVLVN
                     GVRQTNIGVFRAYLTAYLQNNPYLHQEESLMVRQLQPTETGLPLEIYAFSKEQNWEKY
                     EAIQSDIFDHILAGIKYFDLQLFQSPSGKDFSSTIISDFDE"
     gene            complement(11962..12666)
                     /locus_tag="PKZ74_04195"
     CDS             complement(11962..12666)
                     /locus_tag="PKZ74_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91989.1"
                     /translation="MNQRIFLETIGTIEKKEMLGPVGYKELVLELFLPFPGYHGTTVP
                     DHIKPNSLFAITRSKYTEEKIIRAIQKIKNEGKFTFDGTPGLVTLFNMLNPCIRFKDI
                     ETYDDFPPILRAFGEEGIEFMSNRKIEPFTGIIKVKKYFLVEVLSDYVFKDVEDENMQ
                     YFTIPVQLRFPQFEKITLDIKNNLTDPKFDAALGTIFRKTGLIDIIRIYDDQCTPEKL
                     ETIRNKYLNAIRNLSK"
     gene            complement(12751..13626)
                     /locus_tag="PKZ74_04200"
     CDS             complement(12751..13626)
                     /locus_tag="PKZ74_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPM91990.1"
                     /translation="MEKRRICFIHNPVSGPRKLARFEDQVRQNLDLDKYDFTIRETGS
                     PKDAMRLASAAVKDKYDIVVAVGGDGTINEVIQGIDQSDVILGIIPGGSGNGLARHLE
                     LPLEPEKAIQLINKLNTRTIDLATINGYPFASIAGLGFDARVANKYRKVKKRGFFGYF
                     RVIAMEFFRYRAREFTLIFNDMVIKRRALLLSIANSNQFGYNTVIAPAARADDGLLDV
                     VIVKKFPVGELPRIIGLLFTGKIDQSAYIESFKTKEIFIIRKRGKRVNIDGEAVKMGK
                     EIFVRILPGKIKVIV"
BASE COUNT         3302 a         3198 c         3307 g         3959 t
ORIGIN      
        1 agtggtattt ttcagggcca tcatctcctt aagcttctgt atcttgtcga gggtgccctc
       61 aataaaactc tgtccgccga atccgggatt gaccgacata agcatgacca ggtcgacgtc
      121 agggatgatc tcttccagca gcgagaccgg ggagtgggga ttgagcacaa cccctgccag
      181 ggcgccgagt ttttttattg ctccgacggt ccggtgcaga tgggtgcagg cttcgtagtg
      241 cacgctgatc acctctgccc catggtccct gaacttctcg atgtaccggt cgggatcgac
      301 gatcatcagg tggacatcga gcggcttgcg cgcatgtttc tgaatgtggc ggaggacagg
      361 gaatccgaaa gagatattcg gaacgaaaac accatccatc acatcaatat ggaaccagtc
      421 ggcttcactt tggttcacca tcccgatgtc gcgttgcagg ttgccgaaat cggcgctcag
      481 catcgaaggg gcgatcaatg gaaaagtatt ttcagaaaag ttgatcataa ataataatgc
      541 tatcccagat aggacttgag aattttgctc cgtgacgtat gtttcagcct tcggatcgct
      601 ttttccttta tctggcggac tctttcgcgc gtcaggtcaa acttttcacc gatctcttcc
      661 agcgtcatcg ggtgtttgga attcaatccg aaatagagcc tgaccacgtc agcttcgcgg
      721 gatgtcagcg tggaaatggc cctgtcgatc tctttccgga gcgattcgac catcagctct
      781 ttttcgggag tgttggtatc gtcgccacgc agcacgtcat acagcgtatt ttcctcgtcc
      841 tggatcaggg gcgcatccat ggagatatgg cggcctgagt tgcggatcga ttctttcacc
      901 tcgctttccg ggatatccag catttcagcg atctcgacag aatcgggttc gcgttcgttc
      961 tgctgttcca gccgggcata ggctttatta atcttgttga tcgatccgat cttgttgagc
     1021 ggcagcctca caatcctgga ttgttcggcc aatgcctgca ggatcgattg ccgtatccac
     1081 cacacggcat aggagatgaa tttaaatccg cgggtctcat cgaaccgctg ggcggctttg
     1141 atgagaccga gatttccttc attgatcagg tcaggaaggc taagcccctg gttctgatac
     1201 tgttttgaaa cggaaacgac gaaacgaaga ttggccttgg ttagtttttc cagcgcgtct
     1261 ttatcaccgg ctttaatgcg ccgggctaat tcaacttctt cttctgccgt aataagttca
     1321 accctcccga tctcctgcaa atacttgtcg agtgatgcag tttcccggtt ggtaacttgt
     1381 ttggtaattt ttaactggcg catagttcgg tttatgggag tacaaagcta acgtaatttg
     1441 tacgatcccc gcgcagttaa gtttcaaaaa aattactgct tctggggcgg ttgcggtatc
     1501 agcgcttttc tcgacagttt gagcttaccg gtgctgttat ctatgccaat cagcttcact
     1561 tccagcatct gcccgacctt catgtactgg tcgatgtcgg ttacccgttt ccagtcaatt
     1621 tcagaaatat gcaacagacc ttctttgccc gggaggattt cgacaaaagc tccgaaagcg
     1681 acaatgcttt tcactttacc attatagatt tcaccgactt ccgggatggc gacgattttc
     1741 ctgatccgct ctttggctgc ctcgatcgat tctttgttac cggagaagat atcgatgacc
     1801 ccgacatcgc ccacctcttc gatgatgatg gtcgaaccgg tgtctttctg gatctcctgg
     1861 atgatcttgc cgccgggtcc gatcacagcg ccgataaatt ccttgggaat ggtcatcttc
     1921 tcgatcctgg gcacatgcgg tttgtaatcc ggccttgggc tgtcgatggt tttcagcatt
     1981 tcgccaagga tgtgatggcg gccgcgttta gcctgctcaa gggcctgctc catgatttca
     2041 taggacaggc cttcgatctt gatatccatc tggcaggcgg tgatcccatc gaccgtaccc
     2101 gttactttga aatccatatc gcccaggtgg tcttcatcgc cgaggatatc cgataatacg
     2161 gcatacttgc cgcttttcgg gtcggtgata aggcccatgg cgattccgga gaccggtttg
     2221 tggattttca cgccggcatc cataagggcc agcgttccgc cgcaaaccga agccattgat
     2281 gatgatccgt tggattccag tatatccgac acaatacgca ctgtgtacgg attgatgtcg
     2341 tcggccggga tcatgggttt gatggctcgc atagccaggt tgccatgtcc gatctcgcga
     2401 cggctggttc cccggatagg ctttacttca ccggttgaaa agcccgggaa attgtaatgc
     2461 agcataaagt cgcttttgcc ttcgacaacg gcgccgtcaa gcacttgttc gtcgagtttt
     2521 gtgccgaggg taacggtcac cagggcctgg gtctctccgc gggtgaatat agcggagccg
     2581 tgggccgccg gcaggtaatc cacctcgcac cagattgggc ggatgtcttc cagtcctctg
     2641 ccatcgagcc tgatccggtc gtcgagaatg caattcctga cggcattccg ttcttcctcc
     2701 tcaaaatagc ggctgatcat cggttcaagt tcttcccgtt tctcttcggg tattgttccg
     2761 ataaatgctt cttttacagc agaaaatgca tcatgccgct gatgtttgtt gttcatcccc
     2821 tggcgggcga tatcatagaa tttgggataa gcatattcat gaatagctgt ctccagttct
     2881 ttatcctcaa cgggttcggg caccggcatt ttttccctgg ctgcttcgac catttccgcc
     2941 agttcaattt gagcctggca ttgctgtttg atggcttcat gggcgaattt aatggcattg
     3001 agcatgacgg cttccgagac ttctttcatc tggccttcta ccatcactac attatccatg
     3061 gtagcagcga cgataaggtc gaggtcggct tcttccatcc gtgcgatagg agggttgatc
     3121 tcaaattcac cgttaatgcg ggcaacgcgg gcttccgaga ttggtcccag gaaggggata
     3181 ttggaaacca taatggcgct ggaggcggcc agtgcaacga gggtgtcggg caggttcttc
     3241 ttatcgccgg agatcagcat aacctgcacc tggatctcat tgtagaaatt atcggggaac
     3301 aggggcctga gcgcgcggtc gaccaggcgg gagatcagaa tttcatattc agatggacgg
     3361 gcttctcttt tgaagaaacc gccggggaat tttcctgtgg cagagtattt ctctttgtat
     3421 tctacggtga gcgggaaaaa atccgcattt tccataggtt cgggtgcaac aaggactgta
     3481 gccaggagca tggtgtcttc catttttacg acaacagcac ctgcagcttg tttggccaga
     3541 cggccggttt cgatggagac ctcacgtccg tctcccagtt tgaatttttt aacgattgct
     3601 tgtgacattt ttctcttctt tttttccgat tgataaatac aaaaaaaggc aatgctgaga
     3661 ttgccttttt ggtctatatt attgacaata atatttactt tctgatgccg agacttttga
     3721 cgatggcccg gtacctgtcg atttccacat ctttcaggta atcgagcagg ctgcggcgtt
     3781 taccaacgag cgttaccagt gatttacggg tgacattgtc tttcttgttc tgtttcaggt
     3841 gctcggtcag gtaattgatc cggtgcgtga acattgcgat ctggccttcc ggtgaacccg
     3901 tatccgtttc cgatttcccg tgagttgcaa aaaactcttt tttcttttct gacgagagat
     3961 acatatctat cctggtatta attttttaat ttgagcgtgc aaagatagga aaataattta
     4021 gttagacaac tgcaggggga tattttttta gttgttttaa agaaacatta cccaggaacc
     4081 agacattctc taaaagttcc atttcagccg ggtatagcaa agggatccga aatcgccgaa
     4141 ttctgtcata tccttccccc agaagacctg acctataatg aaaaaagtga cgttattcga
     4201 cagggagatg tcgaccgatg gaccggcaaa tcccgattta tcgttcggat tgtaaattgc
     4261 tgagatatcg gcccggatca gtggggtaac agggtatcct atgccggcat aaacggaatg
     4321 tttcgccagc gtgaactttt tggccgagat atcggcataa agggcaagtg cattgccata
     4381 acccgcaggt ccggtcgtcc cgtcgctgtt aaacagatat gaagcattga tgaacagcga
     4441 attcctgaaa gtgtaattcg cgctgactga accgactaaa attccgctgg tgtcttccag
     4501 gctttcccgg ggatggaaat agctgatttc accggtaaaa ccagctcctt taatatcgcc
     4561 cgaccatccg gcccctgcga cgatatccgt tgtcatgact cctgcaaagg cctggaaatc
     4621 atagttccac ctgttgaacc ggtacatgat ggcggatgtg atatcttgct catcatccat
     4681 tttaaaaccg aactgaacgg aagaagtttc attcataaaa tactccagca ggatcgcatc
     4741 gcacccgggc ctttcaatat aatcgaaatc aaaatagttg aaggtgttga aaatatcatt
     4801 cggcgtccag accatattga tggcccagtt gatacgctgc cgtccgatcg tcgcttcaaa
     4861 cttgcctttc ctgattttta tattggcccg gtcgatattc gaataaaaat agtacgaggt
     4921 gtcgctgacc cacagccggg tcagatcgaa gaacccatct tcggtaaccg acatcttagc
     4981 ataataggga taaaaggtct ggaccatctg accgacattg agactgttcc tggctcctgc
     5041 atggaaagag attgtgttcc cgggattcca tcgaaaatcg aaccggttcc cgatcaccgt
     5101 catcgattgc caacggtcgg attcaggcgg tatccagaaa ctttgcaacg attgcaggtg
     5161 cccgttcagc cgccagttcc tttcactttg ctgcgatgaa acattgactg ttaatgatat
     5221 gaagaccagg acgggccata atcttaacca tccgtgaaga ttcgttgcat ccgtttttat
     5281 ttgcgttcca ctaaccgaac acggatgaca ctgattttga cggattttca ccgattcttc
     5341 aattgcgaat ttcatctttt gcaacttttc catcttcaat ggttacaatt ctccttgccc
     5401 tttccataac cctggaatcg tgggtagaga acaaaaaagt ggttttgtac tgccggttca
     5461 ttttttccat gatatccagc agttccgccg tgcttttact gtcgaggttg gcggtgggtt
     5521 catcggccag gataaaccgg ggttttgaag cgagagccct ggccaccgaa acgcgttgct
     5581 gctggccacc cgaaaggtcg ccgggccggt tgtggatttt gtcggccaga cctaccgctt
     5641 cgagcagctc cacggccctt tgttcgcgtt ctttcctgcc gactccctgc agctgcagga
     5701 taaattccac attctcgcgg gcggtcagta ccgggataag gttgaacgac tggaagacga
     5761 aaccgatgtt gtgcaaccgg aaatcgataa gttttcgtga agacagaccg gtaatgttgg
     5821 tgccgttgat atgcacctct ccgctggtcg gattgtcgag cccacccagc atgttcagca
     5881 gcgtcgtttt tcccgatccc gacggcccga ctatcgctgt aaattcagct tcatgaaact
     5941 ccagcgtgat cccgtttaaa gcattgacag ggattttggt ttcatcgtaa accttttgca
     6001 gattgattgt tctgatcgtt gtcattttgc gggttgtaag gttgttaagg gttgtaagtt
     6061 taacatatca cttgttcata atttcaaaat atcacaatat caagaatcct ttcatgcgtc
     6121 ttcacggatg gcggatgcgg gattcagctt cagcgctttc cgtgcagggt atatcgaaga
     6181 gataatcgct gtaatgatca ccattatcgt cattccgaca taaaaatcaa gataaagtga
     6241 aggatagacc agggcactgt agcccagcga ctcatatcct tcagtccatg cagagaaatt
     6301 gatgccgctc attcctgtga agtgtattgt aacggcactg atgataatac caaagaatgt
     6361 acccacgagc gaaaggtaaa tcgtttcggt catgatcatg aggaagattt ttctcctgtt
     6421 cattcccact gccataagca ttccgagttc ccgggtcctt tccagcacgg ccatggtcat
     6481 ggtattgatg attccgaaag ccatggcgat gagtatgatg atcagcagga agtacccgaa
     6541 ctgatccatc attgctgaca tggcaacgag caacggttcg atttctttcc atgacatcac
     6601 gctgagacct ggcagctttt gcttgactgc gtccgacacg ggatcggtca attctgattc
     6661 atcgaggagg attgtgaatt cgcttacctt tccggtgtca aatccggtga gttccgccag
     6721 gtcttccttt ttcacaaaga tgttgggttc atcgaacagg ttattggcag ttttataaat
     6781 tccggtgacc ctgaaaaggc cataggcaat ctcacccgac gtatgctgga tcgtaacgat
     6841 gatcttggaa tggagttttg ctttcagttt ctcacaaagt tttttgctga taacgacggg
     6901 agcgctgttg cttttggttt caaaatagtc gccttcaatt atgtatttgt gtatctgggt
     6961 taccgtattt tcctgttcgg gatcaattcc gtttattgtc acaccggcag ctgtggctgc
     7021 agtgctggcc atagccatgg ccttgatctt gccggatact gcctttacgc cgctgatggc
     7081 ttttaactcc cgtgctattg cattggctga atcaatggaa taggataatg agggttcctg
     7141 aagataatca ggatgatgca cctggatatg tgaaatctcg ttatggattg acgcgtcgat
     7201 cttttgctcc accatgccat tggaaatagc catcatcagc agcgtgccga acaagccaag
     7261 gcataccgca agtatgacta ccaggcttct gagcttattt ctccagatat tgcgccatgc
     7321 gatgaaaatt atcataatga caaatattaa aagttatgtt cagatttgat tttatcttcc
     7381 tttaatggca ttgattattt tgaaccggtt gataaccacg agcggataca acagcgtcag
     7441 catggaaatt atcattacca ccatcgactg gttgataatg aatccgggtt cgagcaggaa
     7501 aggcatgacc ggatccatgt tgtattctgc catggcatcg gcggcctcgc ctttaagcgg
     7561 tagcggattc agatggaaat agtaaattac cggcagactg agaataacac cggatagtat
     7621 gccaatgaag ctgatgatca aggtttcaat gacaataatg atagatagtt tgtatcgtcg
     7681 catccctact gcggtcatga tgccgaattc acgtttgcgt tccattgtgg tcatcagaat
     7741 ggtgcccagt atgccaaatc ctacgacaag ataaaggata ccgagcataa acagaccgct
     7801 gacgttatcc ccctcgaaac tctgcaccag ttccgtgagc aaatctttcc agggcatgac
     7861 ctccaggttt tggggcccta aagccgcaag gtcgttccgg gtcctgtcga gatcttccgg
     7921 atcattgagt atgaaagaga tcgatgtcag gcgtcctggc gcagaataga tctcctgagc
     7981 cagggagaga gggacatatg ttaacaggtt attcatatcg ggctgagtga atttgacgat
     8041 gccaccaatc ggcaatgcgg cagcagcagt cacaccgtgg taaccttgcc cgagaatgac
     8101 aaccgtatct ccaacggtca gtttcaggta attggcaagg ccttcggtca ggatgataaa
     8161 tttatcgtca gtagtgaagt atttaccggc gatgatcttt ttctgtaggc ccgataccaa
     8221 atcttccaca tcgggatcga ctccggtaag ggcagcacct ttggtctgat tcccggaaga
     8281 aagcagcgca aacgattcga gctttggtac aatataagtc acattgggat ggctcctgta
     8341 cttctgttcg aggttgctgc tggtttcgaa ggaattgtcg agggttttat cctcccagta
     8401 tccttcctgg tgaacctgga tatatcccgt tgtgctttta atggtacttt cgatcatttt
     8461 attccaggtg cccagatgca tcgaacgtac gatcagcgca aggagcaaag cgaataatac
     8521 cgatgcaatt gtgagcaggg tcctgcgttt gttgcgccac aggtttctcc atgctatctt
     8581 aaaatattct ttcatcttat tttatattct tcatattttg ctgagaaaag aaactttcat
     8641 cgataggttt gtcgaaaacg atatcgatcg tctcaagaat ggtctggtta cccggtttat
     8701 cgacaggaac catgaccatt cttgaaggaa gggttcggtc gcccattttt ttaatattgg
     8761 aagatgtcat ggtattgacc aggaccaggt cttcatcata atattcccct tttaactcat
     8821 aataatcatt cttggatatc catagtttga ttttgcccca gaccaccgcg gcttccggtt
     8881 tggggattag ctcgatgata tggcaatcaa atctttcgat catttctgat ccaacgatag
     8941 tatgtgtaaa atcatcgatg atcgagttca tcctgaccag gtcgtcgttc gtgaaatcgg
     9001 aacccatcca cgattgcccc atcatcgaag gggggagctt gatcatccgg ttgatgccgg
     9061 gcatccagtt ccacatatcc tgttcccgtt tcataaatac ctgcccccgg tctttcaccg
     9121 gttcagtgat gtagatcagg taataatcgg tccctttcga ccaggatttc atagcgacct
     9181 ccctcgacca gtcgggccgt acgatggtca ttttcatggt accctggctg gtctggccgt
     9241 tggctttgtc gtgcgctttt ttaacgattt cgcgtgcggt gagatcctgg gcaatggaag
     9301 gaaaggccgc gaggagagta cataagatcg tcaggattga atagagccgg tggaaagact
     9361 gtttcataaa agtaggtgtt taaattttaa ttcttaattc ttaattctta attcttaaat
     9421 tttaatgtat tgtattggga tgattattta aaacgattga tgataaggga tttcatttta
     9481 tcaaggggaa aattttcggg ggacatgaca tagttcagga agatgccgtc gatcaggctg
     9541 tcgaaaaata ctgcttccag ttggggatca ttaacacctt tacgttcaaa gtacccttca
     9601 acgatttgtt gaagaggggg cagaaagtgg aggtatttgg ctttgaccag cttataaaca
     9661 ggcggttgca tcatggtcga aaaatacagt ttccaatagt ccagattttc ctgcaaaatg
     9721 cgaaaattcc cgttaataaa gagctcaaat tcttcggacg ttaaaaaacc atcatggtcg
     9781 gggtcgagat gttcggtgag ttcccggatc cccttaccga taacggcgag gataaggtct
     9841 tccttgcttt cgaaataatt gtacatcagc cctttggata tccctgcttt ctctgcaatt
     9901 ttactgatgg aggtcgggta gtatccttca ttggcgaaca actcgagcgc tgttgccagg
     9961 atcagggccg ttttctgttc cctgatttct tcgaattgag attctgttcg aggcatactg
    10021 ttttatgatt gaacggtcgg tcaaaaataa aatgtttttt ttgctgacgc aagaaaaatt
    10081 taatttaatt taccaaaggt aatttgtggt ttataaaaag ttaaactgga ccaatcaatg
    10141 gtttactggc ggtcagtttt tgcgaacagt ttgttcaatg cgtaccggag tgcatcggga
    10201 ttttgtgttc ccagcatgaa ccgttttccg ttcttaaggt cgaactgggc gcccatattt
    10261 ccttttacat tgtaagcggt acctttcaag ccataacgga ctccccatcc gccgtactcc
    10321 attaccgggt tgtacttttt tacttcataa gtttttacat cttccggttt gatcagtttc
    10381 ggttttcgtt gaaacggttt gaaccggtat ttgacccctt gttcgtcgat aaccgtttca
    10441 aggatcataa agcggaacaa aagcaacgcc cctaacggga agagtccgag gaggattacc
    10501 acgaaatcgg gcgcggggtt atcgccaaaa ggaataccca taaagatctg ctgaacgagc
    10561 tgccagatcc agaatccgct taataacagt atgatcagcc atatccacca ttggttaaaa
    10621 cgctgaattt ctttgtataa cactctactc atcgaaatct gaaattattg ttgatgaaaa
    10681 atctttcccg gaagggcttt ggaacaattg caggtcgaaa tactttatac cggccaggat
    10741 gtgatcgaaa atatcggatt gaatggcttc gtatttttcc cagttctgtt ctttgctaaa
    10801 ggcatagatt tcaagcggca gacctgtttc ggtaggctgc agctgacgta ccatcaacga
    10861 ctcttcctgg tgaaggtagg ggttattctg aaggtaagcc gtcaggtagg ccctgaatac
    10921 cccgatattg gtttgccgga cgccgttgac cagcacctcc ggatcgatac ctttatctat
    10981 attgtattga ttcagtctgg cttcggtttg ggtgatatag tcgctgacat attcatattt
    11041 tgagaatcgc tccagcattt ccggcgtgca gaatttaatg ctgttcaggt cgatcaggat
    11101 atgccggcgg aggcgccgca ctcccgattc ctgcatgccc cgccagtttt tataggaatc
    11161 gctgaccagg aaataggttg ggatggtagt gattgttttg tcccagttct ggactttcac
    11221 ggttgtaatg gcgatatcgg tcacgatccc gtcggcatta tatttcggca tttcaatcca
    11281 gtcgccgatg cggagcatgt catttgtcgc cagctggatt ccgcctgcaa aaccgaggat
    11341 aggatctttg aaaaccagga gcagaacggc tgagaaagca cccaggccgg caatccatcc
    11401 gaatcctttt gagcggagaa aaaggatggt gatgatcaca atggcataga cgacatataa
    11461 gaaaatcttt ccgacctgga taaatccttt gatcggcctt gtctttgata tttcgtacga
    11521 gttatagata tcgtgggcgg cttccagcac cgatgatccg acgataacgc agaccgtcgc
    11581 catataaaca ttgatcagtg tgagcaccac caccatcgtg ttcgggtagt cggggatcac
    11641 gtatggtgtg gcccggtgga tgatcagtgc cggaatgagg taagccaggc gcttgaaaac
    11701 tttccggttt acaaaagcgt catcccagtt ggatgtcgac cgggaagcca gccgatggat
    11761 ccatctgacc aggattattt tggcaatata gaaaatgatc agggcgaagg cgatgatcag
    11821 caggaagccg gaatagtctg caatgatctt ggccgggttc tccgacatac ccgaatcaat
    11881 cagccagtct tttaaatacc gggttatcga ttcgaatata tctttattga tttcgatttc
    11941 cttcattgca cgtaaagtgg tttatttgga aagattccgg atggcgttca ggtatttgtt
    12001 ccggatggtt tccagtttct cgggtgtaca ctggtcgtca tagatccgga tgatatcgat
    12061 cagccctgtt ttccggaaga ttgttcccaa tgccgcatca aacttcggat cggtgagatt
    12121 gttcttgatg tcaagggtga tcttttcgaa ctggggaaac cgtaattgta ccggaatcgt
    12181 gaaatattgc atattttcat cttccacatc tttaaaaaca taatcggaaa gcacttcgac
    12241 caggaaatac tttttaactt taataatacc ggtaaatggc tcgattttgc ggttgctcat
    12301 aaattcaatt ccctcctctc cgaaagctct caggatgggt ggaaagtcat catacgtttc
    12361 aatatcctta aaccggatac aggggttaag catattgaac agagtaacca gacccggggt
    12421 accatcaaag gtaaacttac cttcattttt aattttttga atagcccgga tgatcttttc
    12481 ttcggtatat ttcgatcgtg taatggcaaa aagggaattg ggtttgatat gatccggaac
    12541 ggtggttccg tgataacccg gaaatggaag gaagagctcc agtaccagtt ctttataccc
    12601 gaccggtcct agcatttctt ttttctcgat ggtgccgatc gtttcaagga atattctctg
    12661 attcataatg tgcagtttaa gattctgttc tttaggagca agtatagatc atttttgatt
    12721 gtgaataaac taacaggatg attttaccgc tcaaacaatg acttttattt ttccgggcaa
    12781 aatcctgaca aatatctctt tgcccatttt caccgcttcc ccgtcgatat tgaccctttt
    12841 tcctctttta cgaataataa aaatttcttt tgttttgaac gattcgatat aggctgactg
    12901 gtctattttt ccggtgaata gcaatccgat gatcctcggg agttcgccga cagggaactt
    12961 tttaacgatg accacatcga ggagcccgtc gtcggctctg gcagcgggtg ctattaccgt
    13021 attgtatccg aactggttgg aattggcgat agacagcagc aaagccctgc gtttgatgac
    13081 catatcgttg aatatcaacg taaattcccg tgctctgtat ctgaagaatt ccatggcgat
    13141 cactctgaag tagccaaaaa agcctctttt tttgactttc cggtacttat tggctacgcg
    13201 ggcgtcgaat cccaggccag cgatgctggc aaacggataa ccgttgatgg tagccaggtc
    13261 aatcgtacgc gtattgagct tgttgatgag ctggatggct ttttcgggtt ccagggggag
    13321 ttccaggtgg cgagccagtc cattgcccga tcctcccggg atgatgccga gaataacatc
    13381 cgattgatcg atgccctgga tgacttcatt gatcgtacca tctccgccaa cggcaaccac
    13441 gatgtcgtat ttgtctttga cagcagcgga ggcaagcctc atggcgtctt tcgggctccc
    13501 ggtctcccgt atggtgaagt cgtatttatc aaggtcaaga ttctgcctga cctgatcctc
    13561 gaagcgggcc agctttctcg ggcccgaaac aggattatga ataaaacaga tccggcgttt
    13621 ttccatctaa agtcaatcct tgaaaatacc atgcaaaggt acacatttgg ttgacccggc
    13681 gtattgtcca ttgtccattg tccattgtcc attgtccatg tttttcccaa ttcacttttc
    13741 actatttact attcccacac tccatc
//
LOCUS       DAOPNH010000051        13686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4553_length_13686_cov_5.200426, whole genome shotgun sequence.
ACCESSION   DAOPNH010000051 DAOPNH010000000
VERSION     DAOPNH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4553_length_13686_cov_5.200426"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKZ74_04205"
     CDS             <1..1497
                     /locus_tag="PKZ74_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM91991.1"
                     /translation="SFQRNRNPFIDNPQFAGLIWGNITLSSVSIGNVHQSPEIPVAGQ
                     PVTITSTITSSAGAITNATLAYGTSWSNLNQNVTMQGSGNAYSAVIPAQSQGTTVYFK
                     ITAQNNTDNASTVIYNFYVPKTFTGTLTSIYDIQGQGPVSPYLDQVVSVSGIVTGNYG
                     ANYFLQDGSGAWNGLFIYDQGRNPSIGDSIIVTGTIAEYYEKTELKSITDYYFISGNH
                     PLPAPAVITCSQAGEAYEGVLIRVNNATCTDDNYMANYYMWTVNDGTGDLLVHNTSVF
                     EYEPSEGQAYNVSGPLDYDFDEWKIQLRLGTDVTTGGDVSAPSVLSVVVLNDSTLKVT
                     FSEPVSTASGENISNYSVNNGVTVIKARLHEFVKSELYLTVSKLTGGSYNLTIQSVAD
                     LNGNIMPSPAVLPFSTSFGMDEALSFSCKIYPNPAHGSFRIELPDNIKMPVSVTIFSV
                     DGREVYSDRWNNNGRSSSTIAVNDLPEGMYVVELTSDRMISHTKLIIK"
     gene            1510..2244
                     /locus_tag="PKZ74_04210"
     CDS             1510..2244
                     /locus_tag="PKZ74_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPM91992.1"
                     /translation="MSDSLVIIPTYNEKENIEKILRFVFGLPREFHVLIVEDNSPDGT
                     ADIVKRLIPEFPDQLFIEERKGKLGLGTAYIHGFKWALARGYDYIFEMDADFSHNPAD
                     LIKLYEASKIHNADVVVGSRYIKGVNVVNWPMGRILMSYYASAYVRFVTRIKVRDTTA
                     GFVCYTRKVLEMINLDRIRFIGYAFQIEMKYTAWKLGFKIYEEPIIFTDRTEGQSKMS
                     SGIFKEAIFGVISLRLRNIRKIHRKI"
     gene            2323..3699
                     /locus_tag="PKZ74_04215"
     CDS             2323..3699
                     /locus_tag="PKZ74_04215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPM91993.1"
                     /translation="MKDSWYIHNARIVNEGEIYDGGLIIRNGLIERIIHGELPNEELH
                     LVNGLPSLDAGGKYLIPGAIDDQVHFREPGLMHKGEIYTESRAAVAGGVTSFMEMPNT
                     NPQTLTQQLLEEKYRIGSERSLANYSFYMGASNDNLAEILKTDARSVCGLKVFMGAST
                     GNMLVDNHDTLENIFSRVNLLIATHCEDEPVIRANMEQFRELYGEDVPVMAHPLIRSE
                     EACYRSSSLAVQLAEKYNSRLHILHLSSAHEMVLFRNDVPLAEKRITAEVCVHHLWFS
                     DIDYTTLGNRIKWNPAIKSAHDRESLWKALLDDRIDVIATDHAPHTAEEKSGKYFKAP
                     SGGPLVQHSLPAMLDFRKQGRISLERVVEKMCHAPAECFKVEKRGYIREGYWADLALF
                     DDNDPWEVRPDNIYYKCGWSPFEGHTFGSRITHTFVNGNLVYEAEPRILNGNFNEEIR
                     GMRIMFNR"
     gene            3710..4144
                     /locus_tag="PKZ74_04220"
     CDS             3710..4144
                     /locus_tag="PKZ74_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91994.1"
                     /translation="MKKLVFFLVIVVLASCGKKYEEKVIETYPGEKPKRVQYYTTDTD
                     NKYKAKEVFYYENGQKKMEGEYNRDGKKHGKWFYWREDGKMWSEGYFYEGKDDRMRKT
                     WHENGKKHYQGKYDKGVRVGKWKFWDESGKLVKEIDYDKEKE"
     gene            complement(4160..5191)
                     /locus_tag="PKZ74_04225"
     CDS             complement(4160..5191)
                     /locus_tag="PKZ74_04225"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM91995.1"
                     /translation="MRKNIISIYTFIILTLNIQLALSQPNEGKFFRIAFYNVENLYDT
                     IDDPMTDDQDFLPGSRIPWTAERYETKLDHLAKVIADLSESQPVAVFGLCEIENRKVL
                     DDLVSSPAILPFGYKIIHADSPDERGIDNAMLYDEGQFQPLAVQHVAVDLPGDKTRNI
                     LYVKGISRKTKGDTLHLFVNHWPSRSEGQEVSEPKRIRAAEVLKSKVDSVFAGNQRAL
                     IVIMGDLNDEPADKSVGGVLQAIAPADKPAAGVLYNLMIPLHREGKGTLYYKDWDLFD
                     QIIISGGFWLKQKGLRYSGSSGNIFSPDYLLFTNKEGVSRPNRTAAKDYYGGYSDHLP
                     VYIDLLIKK"
     gene            complement(5319..5726)
                     /locus_tag="PKZ74_04230"
     CDS             complement(5319..5726)
                     /locus_tag="PKZ74_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPM91996.1"
                     /translation="MPQEKHDSDEVRIDKWLWAVRVYKTRSLATEAIRNGRVKVGGQV
                     VKPSREVHLNDQIDIHLGVYHKTVKVVALLHNRVGAALVPNFLEDLTPEEEYAKLKIQ
                     QEMKPEFRPRGIGRPTKKHRRLIDRLKKTKDFD"
     gene            complement(5736..7040)
                     /gene="der"
                     /locus_tag="PKZ74_04235"
     CDS             complement(5736..7040)
                     /gene="der"
                     /locus_tag="PKZ74_04235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPM91997.1"
                     /translation="MPNIVAIVGRPNVGKSTFFNRLVKSRQAIVEETAGVTRDRHYGK
                     SDWNGIEFSIIDTGGYVEGSDDVFEEEIRRQVELAIGESDIIIFMVDVKDGLTEMDKD
                     VAQLLRKSRKKIFLAANKVDNTYRTFDVNEFYALGLGQVYGVSSINGSGTGELLDELV
                     KEFKDTVAAEIPDLPKIAVVGRPNVGKSSLINALLGEERHIVTPIAGTTRDTIYTPYN
                     SFGFEFLLVDTAGLRKKGKVTENIEFYSVMRAIRAIEQSDICLLMINAEEGLESQDLN
                     ILSLIEKNHKGVVILVNKWDLVEKENNTMRDMEAFIKSRTAPFTDLPVVFTSVTNKQR
                     IFKAMEWARRVYENKYRQVSTSKLNELLLPIIQATPPPQTKGKLVKIKYITQLKMHYP
                     TFAFFCNTPQYIKEPYARFVENKLRENFEFTGVPIQIFFRQK"
     gene            complement(7068..7949)
                     /gene="era"
                     /locus_tag="PKZ74_04240"
     CDS             complement(7068..7949)
                     /gene="era"
                     /locus_tag="PKZ74_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPM91998.1"
                     /translation="MTKHQAGFVNIIGKPNVGKSTLMNALTGENISIITPKAQTTRQR
                     ILGIVNEENYQIVFSDTPGILQPQYRLQQAMMREVESAFEDADIILYLVEAGEMPEQN
                     DIAERIIKTERPVILIINKIDLTDQAALENKINRWQEIFPTADIIPVSALLKANLQSV
                     FRSILEKLPENPPYYDKDELTDKTVRYFVAEKIREKILLNYQKEIPYSVEVAVDQFKE
                     SDNLIHIIAYIFIARESQKAILLGHQGSAIKRVGTQARRDLEEWLGKKIFLELSVKVL
                     KDWRDSEKELRKFGYDL"
     gene            complement(8088..8882)
                     /locus_tag="PKZ74_04245"
     CDS             complement(8088..8882)
                     /locus_tag="PKZ74_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPM91999.1"
                     /translation="MNKTTFHISKMDCPSEERMIRIKLQPQKSIKSMNFDLQGRRLEV
                     YHDGNAETILDALAGLDLGTTLLGTEPADCPVEEPHEKAERNILWAVLIINFSFFLLE
                     LLAGILAKSMGLVADGLDMLADAYVYGLALLAVGGALAFKKRVARIAGFFQLVLALIG
                     FAEVIRRFAGMAMMPDFRTMILVSALALLANIACLYLLQKSRSRDIHMRASMIFTSND
                     VIINAGVILAGFLVHWLGSGYPDLVIGSVVFVIVARGAFRILKLAV"
     gene            complement(9220..9603)
                     /locus_tag="PKZ74_04250"
     CDS             complement(9220..9603)
                     /locus_tag="PKZ74_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92000.1"
                     /translation="MDPGIKKMSSVEDNGFTFYENKFHKAFEFFGIEPGRTLDFNTAY
                     AVRKNVDVIFITTESISKNSAIVHFVGRENYRQTVSNVRQTVDNDQVLINILLSHKVC
                     MNGILQIEKFIHTTFNMKNEKDADN"
     gene            complement(10213..11400)
                     /locus_tag="PKZ74_04255"
     CDS             complement(10213..11400)
                     /locus_tag="PKZ74_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92001.1"
                     /translation="MLIAFCGLYEALGQWNTNGNNIYNTNIGYVGIGNNSPSTLLTVA
                     KSMTEPTITVRNLGGNGGATYTMTDDASGANWKFKATGLGGFKIRDQGNLMDVITIEA
                     NSFANAIYIKSTDNIGIGTSTPDPSAVMDVNSNTKGLLLPRLTEAEIAAISNPADGLI
                     VYNTTDGKFYAFILNQGSWKELSFGTEIITPDNFVCGYSLSVNHVAGNVAPVSKSVIY
                     GTVTNVPGEPTKCWTTSNLGADHQATAANDATEASAGWYWQFNRRQGYKHGGTTRTPN
                     TTWIWPLNENSDWLLTDDPCYLELGVNWRLPTATEWINVEATGGWTNYFGPWNSLLKL
                     HAAGRLDPTNGSLAARGSEAGYRGRIQNNTGDAKVLFMTSSSCYILDAYKADGFNIRC
                     IRE"
     gene            12175..13674
                     /locus_tag="PKZ74_04260"
     CDS             12175..13674
                     /locus_tag="PKZ74_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HPM92002.1"
                     /translation="MLRIRKVKTKSGSTAIQVVQYTGHRAKLVKHMGSAKDDIERDLL
                     ITKAHEWIEKYTLQSNLFPEQKQNVLMVERSECVRVTHDFAYQFFRSCLEECHLTHLP
                     PLLLDLAIIRLIEPASKRRSVELLSRYFEIKYSQRIYRTIPKLISWKAEIEQRAYRVA
                     SQLFKEQFYYVLYDVTTLYFESFKADEIKVQGFSKDNKSQQPQIVIGLLVTQAGFPLA
                     YDVFAGNTFEGKTMLPVVEGFIAKHPDTKPIIVADAAMLDDDRLAELREKGLSYIVGA
                     RLANTDLGTVKKIHGTLNAANGAITRLHSRHGDLICDFSSKRYRKELNDLNKLVQKAE
                     DLVAKQSSGAKAKFIKRVSKEKIELNTALIEKRKLLLGIKGYCTNLPESQLSAQQAIE
                     RYHHLWRVEQSFRMSKYDLEARPIYHQKEDAIRSHVLICFLALIIEKYLELTTQMTLR
                     NIRLLVWNITESHIRDNLTRETFKFTSPAKELMESQLAPLVKRWKLLPH"
BASE COUNT         3607 a         3187 c         3194 g         3698 t
ORIGIN      
        1 tccttccagc gtaaccgcaa tccgttcatc gacaacccgc agtttgccgg cctgatctgg
       61 ggcaatataa ccctttccag cgtatcgatc ggcaatgtcc accaatcacc tgagattcct
      121 gttgccgggc aaccggtgac catcacctcg acgatcacct cttcagccgg agccattacc
      181 aatgccaccc tggcctatgg aacttcctgg agcaacctga accagaatgt gaccatgcaa
      241 ggctcgggaa atgcctactc agccgttatt ccggcacaat cgcagggtac gaccgtttat
      301 ttcaaaataa cggcgcagaa taatacagac aatgccagca cggtcattta taatttctat
      361 gtacccaaaa catttaccgg taccctcaca tcgatttacg acatccaggg acagggcccg
      421 gttagtcctt acctggatca ggttgtctct gtttccggga ttgtcaccgg aaattatggt
      481 gcgaactatt tcctccagga tggctccggg gcatggaacg gactatttat ctacgaccag
      541 ggacgaaatc ccagtatcgg cgacagcatt attgtaacag gaaccattgc ggagtattat
      601 gagaagacgg aactgaaaag tatcaccgat tattatttta tttcaggaaa tcatcccttg
      661 ccggcacctg ccgtgataac ctgctcacag gccggcgaag cctatgaagg cgtcctgatc
      721 cgggttaata atgctacatg taccgacgat aattatatgg ctaactatta tatgtggacg
      781 gtcaatgacg gcacgggcga cttgctggta cataatacaa gcgttttcga atatgagcct
      841 tccgaaggtc aggcctataa tgtttcaggg ccactcgact atgattttga tgaatggaag
      901 atccagctca ggcttgggac cgatgtgacc accggcggcg atgtctctgc cccttcggtg
      961 ctttcggttg ttgttctgaa cgattccaca ctcaaggtta ccttctccga accggtgtcg
     1021 accgcttccg gcgaaaacat ttccaattac tcggtaaata acggagttac cgttatcaag
     1081 gccaggctcc atgagtttgt gaaatcggaa ctctacctca ccgtcagcaa actgacgggc
     1141 ggaagctata acctgaccat ccagtcggta gccgatctga acggaaatat catgccttca
     1201 ccagcagtcc tgcctttcag cacctcattc ggtatggatg aagcgctgtc gtttagctgc
     1261 aaaatctatc cgaatccggc gcatggatcg ttccgcattg agttaccgga taatataaaa
     1321 atgcctgtgt ccgttactat ttttagcgtg gatggccggg aagtatattc ggatagatgg
     1381 aacaataatg gaaggagcag ctccacgatc gcagtgaatg acttgcccga aggaatgtat
     1441 gtggttgaac tgacgagtga caggatgatt tcgcatacca aactgattat taaataaatc
     1501 caaatataag tgtcagatag tcttgttatt atcccaactt ataacgaaaa ggagaatatt
     1561 gaaaagatcc tccggttcgt ttttggactt cccagggagt tccatgttct gatcgttgaa
     1621 gataattctc ccgacgggac ggccgatatt gttaaaagac tgatccctga atttcccgat
     1681 caactcttta tcgaggagcg gaaagggaag ctgggcctgg gaacagctta tattcacgga
     1741 tttaaatggg ccctggcaag gggttatgat tatattttcg agatggacgc cgacttttca
     1801 cacaaccctg ctgacctgat caaattatac gaagcttcta aaatacataa tgccgacgta
     1861 gttgttggtt cacgctacat caaaggagta aatgtggtga actggcctat gggacgtatc
     1921 ctgatgtcgt attacgcttc cgcttatgtc cggttcgtta cccggataaa agtccgcgat
     1981 accactgcag gatttgtctg ttatacacgt aaagtccttg aaatgatcaa tcttgaccgg
     2041 atccggttca tcgggtatgc attccagatc gagatgaaat acacagcctg gaagctggga
     2101 tttaagatct atgaagaacc gatcatcttt actgaccgca ccgaaggaca atccaagatg
     2161 agctcaggga ttttcaaaga agctattttc ggtgtgatca gcctgcggtt acggaatatc
     2221 aggaagattc acaggaagat atagaaattg aaaacttgaa atccggatcc aaaatcatca
     2281 attcattaca aacgactaac gactaacgat caacgactaa tagtgaaaga tagctggtac
     2341 atacacaatg cccggattgt caatgagggc gagatttacg atggtggttt gatcattcgc
     2401 aacggattga ttgagaggat aatccatggg gaattgccta acgaagaact ccatctggtt
     2461 aacgggcttc cctccctgga tgcagggggt aagtatctga ttcccggggc gatcgacgac
     2521 caggtgcatt tccgcgagcc aggtctgatg cataagggtg aaatttacac tgagagccgc
     2581 gctgccgtag ccggcggcgt cacctctttc atggagatgc ccaacaccaa tccacagaca
     2641 ttgacccaac agttacttga agagaaatac cggatcggat cggagaggtc gctggctaac
     2701 tactctttct acatgggcgc ttccaacgat aacctggctg aaatcctgaa aacggatgcg
     2761 cgatcggttt gcggcctgaa ggtctttatg ggcgcctcga cgggcaatat gctcgtcgat
     2821 aaccatgaca cgctggagaa tattttcagc cgggttaacc tcctcatcgc aacacactgc
     2881 gaagatgaac cggtcatccg ggccaatatg gagcagttcc gggagcttta cggcgaagat
     2941 gtaccggtga tggctcatcc cctcatccgg agcgaagagg cctgctaccg ctcctcatcc
     3001 ctggccgtgc aactcgctga aaagtataat tcgcggttac atatcctgca tctttcatcg
     3061 gcccacgaga tggtcctgtt ccgaaatgat gtgcctctgg ccgaaaaaag gatcaccgcg
     3121 gaggtttgcg ttcatcatct ctggttctcc gatatcgatt atacaaccct gggcaaccgg
     3181 atcaaatgga acccggcgat aaaatcggca catgaccggg agtccctttg gaaggcattg
     3241 ctcgacgaca ggatcgatgt gattgccacc gatcatgcac cgcatacggc agaagaaaaa
     3301 tcgggcaaat acttcaaagc gccttcgggc gggccgctgg tgcaacattc gctgccggcg
     3361 atgctcgact tccggaagca ggggaggatc agcctggagc gcgttgtgga gaaaatgtgc
     3421 catgccccgg cggagtgctt taaggtagaa aaacgcgggt atatccgcga aggctactgg
     3481 gccgacctgg ctcttttcga tgataatgac ccgtgggaag tccgtccgga taatatctat
     3541 tacaaatgcg gatggtcgcc atttgaagga cataccttcg gttcgcggat cacccacacg
     3601 tttgttaatg gcaacctggt ttatgaagca gagccccgca tccttaacgg aaatttcaat
     3661 gaagagatca ggggaatgag aataatgttt aacagataat gctcaatata tgaaaaagtt
     3721 agtgttcttt ctggtgattg tcgttctggc ttcctgcggt aagaaatacg aagagaaagt
     3781 aattgaaacc tatcccggtg agaagccgaa aagagtacag tattatacga ccgataccga
     3841 caacaagtac aaggcaaagg aagtattcta ttacgaaaat ggccagaaaa agatggaggg
     3901 agagtacaac agggatggca agaaacatgg aaaatggttc tactggcgtg aggacggcaa
     3961 gatgtggagc gaaggttatt tttatgaagg caaagatgac cggatgagga aaacctggca
     4021 cgagaacggt aagaagcact accagggcaa atacgataag ggcgtccgtg tgggaaaatg
     4081 gaagttctgg gacgaaagcg gcaagctggt caaagagatc gactacgata aagaaaaaga
     4141 atagtttgtt tgataatcgc tattttttaa tcaacaaatc aatataaacc ggtaaatgat
     4201 cgctgtagcc gccatagtaa tccttagctg cggtacggtt tggacgcgaa accccctctt
     4261 tattcgtgaa aagcaggtaa tcaggtgaaa atatatttcc tgaactgcct gagtagcgca
     4321 gtcccttctg cttcaaccag aatccgccac tgatgataat ctggtcaaat aaatcccaat
     4381 ctttgtaata taaagtccct tttccttcgc ggtgcagcgg tatcatcagg ttgtaaagca
     4441 cgccggcagc cggcttatct gccggagcta tcgcctgcaa taccccgccg acacttttat
     4501 cggcaggttc atcattcaaa tcccccatga tcacgatcaa cgcacgctga ttcccggcaa
     4561 aaacggaatc caccttgctt ttcagcacct ctgccgccct gatccgtttg ggttcgctta
     4621 cctcctgtcc ctccgaccgt gaaggccagt ggttgacaaa aagatgcaac gtgtctccct
     4681 tcgtcttcct gcttatgcct ttcacataca agatgttacg agtcttatcg cccgggagat
     4741 caacggcaac atgctgaacc gctaacggct gaaactgccc ttcatcatac agcatggcat
     4801 tatcgatccc cctttcatcg gggctatcgg catgaatgat cttgtacccg aaaggaagga
     4861 tcgccggcga cgacaccagg tcgtcgagca ccttccggtt ctcaatctca cacagcccga
     4921 aaacagcaac tggctggctt tcagataaat cagctatcac ctttgccaga tgatccagct
     4981 tcgtctcata gcgctcagcc gtccaaggaa tccgcgatcc gggcagaaaa tcctggtcgt
     5041 cggtcatcgg atcgtcgatt gtatcgtaca agttctccac gttgtagaaa gcgatgcgga
     5101 agaatttacc ttcgtttggc tggctaagag ctagttgaat gttcaaagtg agaatgatga
     5161 aggtatagat tgagataatg tttttcctca tggttggatc attttattga atttcttccg
     5221 acgactgaag ccgtcggttc ctacctttat aaaacaatag gaaccaacgg ctttagtcgt
     5281 tggagcacga actaataaca ggccgtaaat atagtcattc agtcaaaatc ttttgttttt
     5341 tttaagcgat cgatcaggcg gcggtgtttc ttcgttggtc ttccaatgcc tctgggcctg
     5401 aattccggct tcatctcctg ctggatcttc agtttggcat attcttcttc tggcgttaaa
     5461 tcctcaagaa aattggggac cagggccgcg ccgacgcgat tgtgcagcag ggcaacgact
     5521 tttaccgttt tatggtaaac gcccaggtga atgtcaattt gatcattcag atgcacctcg
     5581 cgggaaggtt tcaccacctg cccgccgacc ttcacccttc cgtttcggat ggcttccgtg
     5641 gcaaggctcc tggttttata aaccctgact gcccagagcc atttatcgat gcggacttca
     5701 tcgctgtcgt gcttttcctg gggcatggtc cggttttact tttgcctgaa gaagatctgg
     5761 atcggtaccc ctgtaaattc aaagttttcc ctcagcttgt tttcgacaaa ccgggcatag
     5821 ggctccttga tatattgcgg cgtattgcag aaaaaggcaa aggtcggata atgcatctta
     5881 agctgcgtga tatattttat ttttaccagt ttgcctttgg tttgcggcgg gggagtggcc
     5941 tggatgatcg gcaggagcag ctcgttaagc ttcgatgtcg atacctggcg gtatttgttt
     6001 tcgtaaactc tcctcgccca ctccatcgct ttgaaaatcc gctgcttgtt agtcaccgac
     6061 gtgaatacca ccggcagatc ggtaaatggc gctgtgcgcg acttgatgaa ggcctccatg
     6121 tcgcgcatgg tgttgttttc cttctccaca agatcccatt tgttgaccag gatcaccacc
     6181 cctttgtggt ttttctcgat caggctcagg atattcaggt cctggctctc cagtccttct
     6241 tccgcattga tcatcagcag gcaaatgtcg ctttgctcaa ttgcccggat ggcgcgcatc
     6301 accgagtaga actcgatatt ctccgtcact ttccctttct tgcgaaggcc tgccgtatcc
     6361 accagcagga attcgaatcc gaagctgttg taaggagtgt agatcgtgtc gcgggttgta
     6421 ccggcgatag gggtgacaat atgccgctct tctcccagca acgcgttgat cagcgatgat
     6481 ttcccgacat tcggccttcc gactaccgcg atcttcggca ggtccggaat ttcggcggct
     6541 accgtatctt tgaattcttt gaccagttcg tcgagcagct cacctgtccc gctaccgttg
     6601 atggaggaga cgccatatac ctgcccgagg cccagggcgt agaactcatt gacatcgaaa
     6661 gtccggtatg tgttatcgac cttattggcg gccaggaata tctttttcct gcttttgcgc
     6721 agcagctggg ccacgtcttt gtccatctcc gtcagtccat ccttgacgtc gaccatgaag
     6781 atgatgatat cggattcgcc gatagccagt tccacctggc ggcggatctc ctcttcaaaa
     6841 acatcatccg atccttccac gtagcctccg gtatcgatga ttgagaattc gatcccgttc
     6901 cagtccgatt taccgtaatg gcggtcgcgc gttacgccgg cggtttcttc gacgatggcc
     6961 tggcggctct tcaccagccg gttgaaaaac gtggatttgc cgacattggg ccggcctacg
     7021 atggctacga tatttggcat gctgcaaagg taacgattga ttttcaatca gaggtcatat
     7081 ccgaatttac ggagttcttt ttcactgtcg cgccagtctt tcagcacttt aacgctcagt
     7141 tcgaggaata ttttcttacc cagccactct tcgagatccc ggcgggcctg tgtgcctacc
     7201 cgcttgatag cgctgccctg gtggccgagc agtatggcct tctgcgattc gcgggcgata
     7261 aagatgtaag caatgatatg gatcaggttg tcgctctcct tgaattgatc cacggcaact
     7321 tcaacggagt aggggatctc cttctgatag ttcagcagga tcttttccct gatcttttcg
     7381 gcaacgaagt agcggaccgt tttatctgtc agctcgtctt tgtcgtaata gggagggttt
     7441 tcggggagtt tttccaggat ggatcggaag accgattgca ggttggcttt cagcagggcc
     7501 gataccggga tgatgtcggc tgtagggaaa atttcctgcc accggttgat tttattttcc
     7561 agggcagcct ggtcggtcag gtcaatttta ttgatgatga ggatgaccgg ccgttcagtt
     7621 tttattatcc tttcggcgat gtcgttttgt tcgggcattt ccccggcttc gaccaggtag
     7681 agaatgatgt cggcgtcttc gaaggccgat tccacctctc gcatcatggc ttgttgaagc
     7741 ctgtactgcg gttgcaggat accgggggta tcggagaaga cgatctggta gttctcctcg
     7801 ttcacgatcc cgaggatccg ctggcgggtt gtctgggctt tcggggtgat gatggagata
     7861 ttctcgccag tcagggcgtt catcagcgtc gatttaccca cattgggctt cccgatgata
     7921 ttgacaaagc cggcttggtg tttggtcatg ggttgttggt tgctggtcct gagggcgaaa
     7981 cccgaaggat cttagttttt gtattttaca aaagtagctt attaatgaat tgaaagacgg
     8041 aactttctaa tagggtacgc attccaccgg agaaaaggca gaaatgctta tactgccagc
     8101 ttaaggatcc tgaaagcccc gcgggccacg atgacaaaca cgaccgaccc gatgaccagg
     8161 tcggggtacc cggagccgag ccagtgaacg agaaacccgg ccaggatcac tccggcattg
     8221 atgatgacat cattggaggt gaagatcatg ctggcgcgca tgtggatatc cctgctcctg
     8281 cttttttgca gcaggtaaag gcaggcgatg ttggccagga gcgccagggc cgaaacgagg
     8341 atcatcgtcc tgaagtcagg catcattgcc atgccggcaa accgccggat gacctctgca
     8401 aatccgatga gggccagcac aagctggaaa aaacctgcaa tccgggcgac tcttttcttg
     8461 aatgccaggg ccccgccgac agccagcagg gcaagcccgt agacgtatgc gtcggctagc
     8521 atgtcgaggc catccgcaac cagccccatc gactttgcca ggatacccgc cagtaactca
     8581 agcaggaaaa aggagaagtt gatgatcagc actgcccaca ggatattcct ttcagccttt
     8641 tcgtgcggtt cttcgacagg gcagtcggcc ggctctgtgc cgaggagcgt cgtgccgagg
     8701 tccaggcccg ccagggcatc gaggatggtt tcagcattac cgtcgtgata aacttccagc
     8761 ctccggccct gcaggtcaaa attcatggat tttatactct tctgcggctg cagctttatc
     8821 cggatcatcc gctcttccga agggcagtcc attttggaaa tatggaacgt ggtcttattc
     8881 acgaccgaaa gttacgtcta tttttctgag attgtacttt tagaacccat gtcctgttgt
     8941 ttgccaccta ttaacggaag ggacgccatc ccttgggatg gcgtcccgat cggtcaatcg
     9001 agatggagtc tcacataaca aattgctgat caggatgatt tattctgctt acctgtatca
     9061 cgcacctgta gaacggtttt ccccgggatt aactctgcca ccccgccagc gcctgtgtga
     9121 tgaaggttag tgtgtggtat caaaaaacaa agtttttaca gtcctcggaa atttccttgc
     9181 cttcattgaa tttcttatta aagtcattcg cagtaataat taattatctg cgtccttttc
     9241 attcttcata ttgaatgttg tatggataaa tttttctatt tgcaatattc cattcatgca
     9301 tactttatgc gaaagcagga tattgatcag cacttgatca ttgtcaaccg tttgcctgac
     9361 attcgatacc gtttgccggt aattctccct gcccacaaaa tgaacgatag cgctattttt
     9421 ggagatgctt tccgttgtga taaaaattac atcgacattt ttgcgaactg cataggctgt
     9481 attgaaatcc agcgttcttc ccggctcaat gccaaagaac tcaaacgcct tatgaaactt
     9541 gttttcataa aatgtaaaac cattatcttc aaccgacgac atttttttaa tgccgggatc
     9601 cataaggtaa ttattgaatt gtttgatcaa ggaattgtct ttgatattat tcagcctgtc
     9661 ctcctttctg caatattgaa aaaacctgct gatctcttgc ttcctgatat caagtcgaat
     9721 aggaataatt attttaattt gaaattcatg ataatattta agtacaacat tgattaagga
     9781 ttgtggatta tgaaggtgca atacattatt tctttttgct tctgctggtg taaggcttac
     9841 atccaggtta atactttcat tgcataatat actattccac agcgaagctg ttccgctttt
     9901 gcctactcca aagacaatat atattatttc tttcagggta aaatatgtta gttaacagca
     9961 aatttaatta aaaagggacg ccatcccttg ggatggcgtc ccgattggtc aatcgagatg
    10021 gagtctcact agtgaaaaca ataagcccgg cctgtaacaa aaggccgggc ttatgtttgt
    10081 tagcggggac aggtctcgga ccaaaatcaa ctatcaatac ggctatctga gcgtcacatt
    10141 gatcccgttc tttttagatc ctgatacgag atctaataag ttgtctgcaa aaatgctagg
    10201 aatgaaaagt cattattccc taatgcatcg gatattaaag ccatctgctt tataagcgtc
    10261 aagtatgtaa caactgctgc tggtcataaa aagcaccttc gcatcacctg tattattctg
    10321 gattcttccc ctgtaacctg cttcagagcc tcttgcggct aaggaaccat ttgtaggatc
    10381 cagacgtccg gcggcatgga gcttaagaag tgaattccac gggccaaaat agtttgtcca
    10441 tccgccggtc gcttccacat tgatccattc agtcgctgtc ggtaaacgcc agtttacgcc
    10501 aagttcaaga taacaaggat catcggttaa taaccagtcc gaattctcat ttaagggcca
    10561 gatccacgtc gtatttggcg tacgtgttgt gccaccatgc ttatatcctt gtctcctgtt
    10621 aaactgccaa taccaaccgg ctgatgcctc agtagcatca tttgccgctg ttgcctgatg
    10681 atcagcgcct aggttgctag tggtccagca ttttgtcggt tcacctggga cattagtaac
    10741 tgtgccataa atcaccgact tgctaactgg agctacattg ccagccacat gatttacgct
    10801 aagggaataa ccacaaacaa aattatcagg agtgattatt tctgttccaa atgataattc
    10861 cttccaggag ccttgattca agataaaggc ataaaacttt ccgtctgttg tgttataaac
    10921 tataaggcca tctgcaggat ttgaaatagc agcaatctcc gcttcggtca accttggtaa
    10981 taatagccct ttcgtatttg aattaacatc catcacagcc gaaggatctg gtgttgaagt
    11041 cccgatacca atattatcag tgcttttaat gtaaattgca ttagcaaagc tgttcgcttc
    11101 tatagtgatt acatccatca ggttaccctg gtcacgtatc ttaaatcctc ccaatcctgt
    11161 cgctttgaac ttccaatttg ctccgcttgc atcgtctgtc attgtataag tagctcctcc
    11221 gtttccgcca aggttacgaa ctgtaatagt gggttcagtc atgcttttag cgactgtcaa
    11281 aagtgttgag ggtgaattgt tccctatccc gacataaccg atatttgtgt tgtaaatatt
    11341 attgccgttg gtattccact ggccaagagc ttcataaaga ccgcaaaaag cgattagcat
    11401 aaaaaaagta ataatcgttt catagtttca atgttttaat gttcattgtt attggtttta
    11461 attagaatgg tcctattgtg aatcgaatca ctatttttat taatatcgtt tatatattca
    11521 ttgaattatt gccgtttgca tttcagataa tctcatttaa ccaacattag aatatcacat
    11581 atgcatggaa catcattatg taaataattt taatcaaata atatttatct aatattaatt
    11641 tgataaataa ttaataccct gatgtgatca ctaaagacaa ctttttgaat aactagcgac
    11701 ttggcagcaa atagaacgta tggggtactt aagtctggta attatttaac attactatta
    11761 agccataaat agaataaaat aaagtgtgga aatcaaatct ttatggaata attttaacat
    11821 aaactttgga aaaataagga gaccgagtgt ataccataat aaaagggacg cggctttctc
    11881 aaaagcctga tatttcacag taatggcctg acattagttc gtgatccaac gactaaagcc
    11941 gttggttctt atggttttac gaaagttgga accgacggct ttagtcgtca gaagaggaac
    12001 acccgttgaa ccaatcggga cgccatccct tgggatggcg tcccaatttg ttgtatattc
    12061 ctgacttgtg acgtttagca gaattagtgc ggtcatattt tttaacatac agaatatgag
    12121 tgatatagaa agcgttaaat tgatttttac cgcactaacg cctatatttg tgccatgctt
    12181 aggatcagaa aggttaagac aaagtcagga tctactgcca tacaggtggt tcaatatacc
    12241 gggcacagag caaaacttgt taaacatatg ggtagcgcca aggatgatat tgagcgcgac
    12301 ctgttgataa ccaaagcgca cgaatggatt gaaaaatata cactgcagtc aaacctattc
    12361 cctgagcaaa agcaaaacgt cctaatggtt gaaagaagcg aatgcgtcag agttacccac
    12421 gattttgcat accagttttt tagaagttgt ctcgaggaat gtcacttaac acatcttccc
    12481 cctctcttac ttgatttggc aatcatccgg ttaattgagc cagcctcaaa acgacgatca
    12541 gtcgagttgc tctcccgcta ttttgagata aagtactcac agcggattta tcgtaccata
    12601 cccaaactca tatcctggaa agcagaaatt gaacagcggg cataccgtgt agccagtcaa
    12661 ctgtttaagg agcaatttta ctacgtactg tacgatgtaa ccacgctgta ctttgagtcg
    12721 tttaaagccg atgaaattaa ggtacaggga tttagtaaag ataacaaatc acagcaaccc
    12781 caaatagtga taggcctgtt ggttacacaa gcaggttttc cacttgcgta tgacgtattt
    12841 gcaggcaata ccttcgaggg gaagaccatg ctacctgtgg tggaaggctt tattgccaag
    12901 catcccgaca ctaaacctat aatagtggca gatgccgcca tgctcgatga cgacaggctg
    12961 gccgagctca gggagaaagg tttatcctat attgtagggg ctcggctggc caataccgat
    13021 ctgggtacgg taaagaaaat ccatggcact ttaaacgcgg ctaacggggc cataacaaga
    13081 ttgcattcca ggcatggtga tttgatctgc gacttctcct ctaagcgcta caggaaggag
    13141 ttaaatgacc tgaacaagtt agttcagaag gcagaggatc tggtcgctaa acaatcttcg
    13201 ggcgctaagg ccaagttcat aaaaagggta tccaaagaaa aaatcgagct taacacggcc
    13261 ctgattgaga aaagaaaact cttgctggga attaaaggat actgtactaa tctgcctgaa
    13321 tctcaactgt ctgcacaaca agctattgaa agatatcatc acctatggcg ggtagaacag
    13381 tcatttagaa tgagcaagta tgatctggaa gcacgaccta tttatcatca gaaagaagat
    13441 gcaatcagat cgcacgttct tatttgcttt ctggcattga tcattgagaa atacctggag
    13501 ctaacaactc aaatgacctt gagaaatata aggcttctgg tgtggaatat tactgaatca
    13561 catatacgtg ataatttaac cagggaaact ttcaaattta cttcccctgc taaggaacta
    13621 atggaaagtc agttagcacc attagtgaaa aggtggaaac ttctaccgca ctaattgtca
    13681 caagtc
//
LOCUS       DAOPNH010000052        13634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4571_length_13634_cov_6.844098, whole genome shotgun sequence.
ACCESSION   DAOPNH010000052 DAOPNH010000000
VERSION     DAOPNH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_13634_cov_6.844098"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PKZ74_04265"
     CDS             complement(<1..1400)
                     /locus_tag="PKZ74_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM92003.1"
                     /translation="MSIRKILIVDDEEIVRYSFRKLFREPGFEISEAANGLDALSAIK
                     KDSPDLVLMDIEMPGLNGLEAIQRIKAMDPQLPVIIMTAFGTSERVIAAMKYGAFEYL
                     EKPFDVARLKAVISDAIETRRISDQDKKFEILPAADTHTGEQIIGMSPAIKEVYKMIG
                     RVAASDVSVLLTGESGTGKELVAKAIHRYSDRSDKPFIAINCAAIPDTLLESELFGFE
                     KGSFTDASHTKEGKFEMADKGTLFLDEVADMSLTLQAKLLRVLQEGTFERLGSNKVMK
                     VDVRVISATNKNLENAIAGKNFREDLYYRLKVISITLPPLRVHPEDIPLLTNYFLSRH
                     CKEMKKPQVTVPPETMEALLRHTWPGNVRELENMLKRALLLSKNNVITPDLVHSEIQT
                     NAPVTKDQPHPAYPSLIPDNMEDFEGVLFKHVIEKVEKEIINKALAHCKGNQVKTAKF
                     LGISRAMLIERMKNYG"
     gene            complement(1403..2899)
                     /locus_tag="PKZ74_04270"
     CDS             complement(1403..2899)
                     /locus_tag="PKZ74_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92004.1"
                     /translation="MRRPRMRFHFSYQMIVWSLVTIAIILAGGFLIISYIYRIQDETT
                     ALMDQNVRSAKTAKELTLSLYDIRAASLTYLFDRSPERIAILQQKQTDFILLLQKAKE
                     SANTEEEKTLIQQISALFSNYQQTLTNALELHKIGRISQPNKLIVHASQDLINTIEEK
                     TNTFIIKNESAQLEYETSIEKNENIIRSAMYALGFGGIFLGIVLGWMIARIILNPIYK
                     LVLKVRDAAGSEVVEHIKMSPGKELEELDVHISRLISRINQANEDLRKNRELLERSSK
                     LAAIGKIAPALAHEIRNPLTAIKMLIYSMMQEPGISNDKLHDLEIITHEINRVEGFLQ
                     NFLKYARPAKPQMQIVQIVPVLKETLQLMQPRFRQNNINLVEIHEQENLKIKADPDMI
                     RQVVMNLVLNAVESMGQEGELRFSTLIRDDDQGTGMFQIVISDTGPGIPDEIRDSMFD
                     PFVKGKDQGIGLGLSISQRIAELHHGWISASNNAGKGATFTVHLPLKP"
     gene            complement(3023..4825)
                     /locus_tag="PKZ74_04275"
     CDS             complement(3023..4825)
                     /locus_tag="PKZ74_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPM92005.1"
                     /translation="MNAAPLIIGAIAFFVLAYRFYYSWIMARALTINDLRQTPAHRLY
                     DGQNYYPMSKWVLFGHHFAAIAGAGPLVGPVLAAQFGYFPGFLWMVLGSVIAGAVHDL
                     VILVASVNHDGKSLAEIARKEVSKLSGGIASLAILIILVVALAGMGVVVVNALAESSW
                     GTFTIAMTIPIAVFMGFWMFRFRKGKTGEATVIGVILLSLAVIYGKHIPDSAVAHWFV
                     FDKPTLTILIAVYGFFASVLPVWVLLSPRDYLSSIMKLAVVAMLAVGIIIVMPEMHMP
                     AFTKFIHGGGPIIPGPLFPYLFITIACGAISGFHSIVSSGTTPKMLMKESHIKFIAVG
                     SMLSEGAVSVMALIAASSLFPLDYFQINVPVEKFAQILPMLQEMGFTQSNLETLSREV
                     GENIAGRTGGAVSLAVGMAQIFSAIPGLEKLMSYWYHFAIMFEALFILTIIDAGTRIA
                     RFVLQEALGKIYKPFGKTSWLPGNLITSLIIVFAWAYFVYTGSVSTIWPMFGTANQLL
                     GTIALCIGTSYLINRGKAKYAWITIVPMSFVGVTTMTAGFLNIKNLFIPQIMEASTRI
                     QGSVNLALTVIIMASLVIILADSVPRWMRVVRGH"
     gene            complement(4831..7203)
                     /locus_tag="PKZ74_04280"
     CDS             complement(4831..7203)
                     /locus_tag="PKZ74_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92006.1"
                     /translation="MLKHFLITGFRIFRRNRAATLLNVAGLSIGLTTVILIMEYVFDE
                     ISYDRFHANQKHIYRVIIKEEKEAEISPTPIITAAIGPSMAEEFREVDKFVRFANPSG
                     CFLATDERNFPLAKLSYADSSLFEVFSFSLLRGDPKKALAEPFQVVLTESAARRIFGD
                     SDPLRQVVRLNNKENLMVSGVMKDFPSTSHLDFDALISFSTLYQDKSLYLDWDGGWNY
                     STYVLLYPGSELSSLKEKFPDFMEKNINYKYRSFGFILHLDLQPLKRIHLFSGKDLGL
                     EGEGDLQALYIFSSIALFILIIACVHFMNLTTARSFMRSREIGMRKVSGASRMTIIFQ
                     FLFETMMISGAAFIIALILADFLQPEFNSLTGKDLSLTGSPGLKMAGGIILLLIVTSL
                     LAGSYPAWFISRFPPLMSIKGTMISGKGKSSLRNILVVFQYLVSLILLIMTLAVFTQM
                     RFMNSLPLGFDKEDVMVIPLVSEEAKLGSRALINAFNSLPPVRAAGASSEVPGEGFTM
                     NGYFPEGFKEPVMINVLDIDADYLGVMNIPLIQGRGFDKASGSDSASYIINETLAKKL
                     GWGDPVGKTIYRDGPHKVIGVVGDFHFASLHQPLQPLILTKQPWIGYNFISVRIDPGN
                     REEAIRRVESAWNSELTGEPFDYFFQSDFIRNSYNGVRHATTAILWFSFLSIIIAGLG
                     LMGLANFTFSLRRKEIGIRKVLGAETPVIARRVTFEFLRLVIIAGVIALPLAWLLMDK
                     WLGNFAYRTMINPMIFILPVIFVLILAWITIYFQVKKLANTNPVNVLKFE"
     gene            complement(7248..8285)
                     /locus_tag="PKZ74_04285"
     CDS             complement(7248..8285)
                     /locus_tag="PKZ74_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HPM92007.1"
                     /translation="MRLIMGFMALLMAGLTGCGPSPQKPSPDAFPGLLDKAKASFSPI
                     TEPVDNPDNPITKEKVILGQTLYFDKRLSKDETLSCNSCHDLQTYGVDNEATSGGVGG
                     QKGERNSPTVLNAAYHFSQFWDGRAKDVEEQAGGPILNPVEMAMPDQEAVLKRLQAVE
                     GYRKLFAAAFPGEEQPVNYINLQKAIGAFERKLITPSRFDEYLKGNTEALTTDEKRGL
                     QTFMDVGCTACHSGTVLGGQMYQKFGLAGNYWDFTQSSRVDNGRYLVTKSESDKFIFK
                     VPSLRNIEKTHPFFHDGSVTDLGEAVKIIAKLQLNRDLTEDEVKDIVTFLKTLTGTVP
                     AEFTRAPEMPM"
     gene            complement(8394..9746)
                     /locus_tag="PKZ74_04290"
     CDS             complement(8394..9746)
                     /locus_tag="PKZ74_04290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HPM92008.1"
                     /translation="MRTLRIITFILTLAFTGCQSTVVKTDDQKIADQVKSEFLRSWKA
                     YKTYAWGHDVLLPLSKSHMDWYENSLGISPIDAYSTMKVMGLDEEAAQVQKYVADTIN
                     FDKDQFVKTFEVNIRVLGGLVAMYQFTKDPKVLAKAEDFGKRLLAAYNTGTGIPAYWV
                     NLRTGEIKGDTVNLAEGGSSLIEMGMLSRFTGNPVFFDAAKKASLAIHARRSSLGLVA
                     ENINVQTGQWVDSVSHIGCCIDSYYEYLLKGWLLFKDEDLKRAWEESLVAIRKFVAEE
                     PDSSLWYGQVNKETGEMVNRVVTLYDAYFPAVLVLSGDLKNAARYQDSWHSLWLKYSL
                     EPMVYDYGKKEILHPAYNLNPEIIESAYYLWYFTGEKKYFEMASQYWYDIMAYCRTDV
                     AFTNIKDVRTKEQADHMETFFLAETLKYLYLIFANPPGINPKECVFSTEAHPFRKPAA
                     "
     gene            complement(9760..10077)
                     /locus_tag="PKZ74_04295"
     CDS             complement(9760..10077)
                     /locus_tag="PKZ74_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HPM92009.1"
                     /translation="MNWIFLIIAGAFEIGWPLGFKLSQTTPYKFGWILFSIAAMGLSG
                     LFLWMAQKTIPMGTAYAVWTGIGAVGAFVTGIILFKDPVTALRIVSVVFIITGIVGLK
                     VAG"
     gene            complement(10160..10693)
                     /locus_tag="PKZ74_04300"
     CDS             complement(10160..10693)
                     /locus_tag="PKZ74_04300"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPM92010.1"
                     /translation="MSTTQWIILISGTILLILFSWFVSLREKRYHGIPRFFVFEGLLI
                     LGLLQADTWFHDPLVPRQIISWLLFISSIFYAFYGIMIFIKKGKPGENFENTTRLVTT
                     GLYRFIRHPMYASLLFMGWGMFLKEINMVTALITAGITLAVYLTCRVEEKEMIARFGE
                     EYREYMKRTRLWIPYLI"
     gene            complement(10785..11687)
                     /locus_tag="PKZ74_04305"
     CDS             complement(10785..11687)
                     /locus_tag="PKZ74_04305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM92011.1"
                     /translation="MMDSENKSCPLCHSGTGKDYIRVRDFFLSGESFSLLKCSNCGFV
                     YTAGVPAPNEIGKYYKSDEYVSHSDTRKGVMNRLYHTARSIMLYLKFRMVRNESEGDR
                     LLDIGCGTGYFPAYMQRRGFGVNGVEVDPAAAQFARKTFGLNVATPAEFLNREIQGYF
                     DIITLWHVLEHLDDFDLYIDRMLEYLLPGGLLVIALPNCSSPDARHYKEYWAGYDVPR
                     HLWHFTPSTFKVLADKHRLKIIKMKRLPLDPFYNSMLSEKYWKSRFFMIKGTIIGKLA
                     YLESLFSLKKSSSVVYFLKRKSDS"
     gene            11885..12886
                     /locus_tag="PKZ74_04310"
     CDS             11885..12886
                     /locus_tag="PKZ74_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92012.1"
                     /translation="MNFPKKVTIGDITVRDGFQHEEKFIPTEAKLWLAEELLLCGFKH
                     IEVTNYGNPKGMPQFKDADELMKALRTSKKVAHLLGEVKLTTITIREKAIERAIQARK
                     EGYGPDRILLMVSTSESHHRTNSGISIEEYFRMAEKYIPMALDAGLKVNGTVSTIWGC
                     PIEGPTEMKKAIEFSRRWFDIGATDVEHADHDGSASPDRVYRYYSMLLDAFGSPDKHI
                     VHFHTTRGWGLANVLAALQAGMTNYESTLGGIGGQPANFVDGVPVPGTGEYYYKDPMI
                     VGLVSTEDMVVMMDEMGIDTGLDIDRLLEAGKMAERIVGRRLRSETIRNGRIPKHMK"
     gene            complement(12925..13278)
                     /locus_tag="PKZ74_04315"
     CDS             complement(12925..13278)
                     /locus_tag="PKZ74_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM92013.1"
                     /translation="MKENIIVIKSFTFSLNVLQLYKELMKQKEFIISRQLLRCATSIG
                     ANIEEAGAASSRKDFALKMSISSKEAREARYWLKLLKEDNFIEGYDFSALMNEVDQII
                     NILTKIVKTAQENSR"
     gene            13384..>13634
                     /locus_tag="PKZ74_04320"
     CDS             13384..>13634
                     /locus_tag="PKZ74_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92014.1"
                     /translation="MITIVETPRDAFQGIKQFIPTERKIRIIDLMMKAGFDTVEAGSY
                     VSEKVIPQMADTTQVLRGLDKKNSRSRVMVLVVNKRGAM"
BASE COUNT         3473 a         3434 c         3293 g         3434 t
ORIGIN      
        1 aatccgtaat ttttcatccg ttcgatcagc atggcgcggg aaatgcccag gaattttgct
       61 gtttttacct ggtttccttt gcaatgcgca agggctttgt ttattatctc cttttcgacc
      121 ttttcgatga catgtttaaa caagacaccc tcgaaatcct ccatgttatc cggaatcaga
      181 gaagggtagg ccggatgggg ttggtctttg gtaacagggg cattcgtttg gatctcgcta
      241 tgcacaagat cgggtgtgat gacattgttt ttacttaaca acagtgcccg cttcagcata
      301 ttctccagct ccctgacatt ccctggccag gtatggcgta gcagagcctc catggtttca
      361 ggcggcacgg taacctgagg ctttttcatc tccttgcaat gcctcgacag aaaataattt
      421 gtgagaaggg gaatatcctc cggatggacc cggagcgggg gcagcgtaat ggagatgacc
      481 tttagccggt agtaaagatc ttcgcggaaa ttcttcccgg caatggcatt ttcgagattt
      541 ttattggtag ctgagatgac cctgacatct actttcatca ctttattact gcccagcctt
      601 tcaaatgttc cctcctgtaa aacgcggagc agcttggcct ggagtgtcag gctcatgtcg
      661 gccacctcat cgagaaagag ggtgcctttg tcggccatct cgaatttccc ctccttggtg
      721 tgcgaagcat cggtgaatga acctttttcg aagccgaaga gttcgctttc aagcagggta
      781 tcgggaatgg ctgcgcagtt tatggcgatg aacggcttgt cggaccggtc gctgtaacga
      841 tggatcgctt tggcaaccag ctccttgccg gtaccgcttt cgcccgtcag caggacgctg
      901 acatcgctgg cggccacgcg cccgatcatc ttgtacacct ccttgatggc agggctcatc
      961 ccgatgatct gctctccggt gtgggtgtcg gctgccggca ggatctcgaa cttcttgtcc
     1021 tggtcggaga tgcgccgtgt ttcaatggca tccgatatga ccgccttgag cctggccaca
     1081 tcgaaaggct tctccaggta ttcgaaagcg ccgtatttca tggcggcgat aaccctttcg
     1141 gaggttccga aggccgtcat gatgatgacc ggaagctgcg gatccatcgc tttgatccgt
     1201 tggatggctt ccagcccgtt gagcccgggc atctcaatat ccatcagcac caggtcaggc
     1261 gaatcttttt ttatagctga caaagcatca agcccgttcg cggcttcgga aatttcaaag
     1321 cccggctcac ggaatagctt tctgaaggaa tacctgacga tctcttcatc atccacgatc
     1381 aatatcttcc ggatgctcat attcaaggtt tcaacggcaa atgtacggta aatgtcgccc
     1441 ctttgcccgc attatttgaa gcgctgatcc agccgtggtg caattcggct atccgctgcg
     1501 agatcgatag tcccagtccg atcccctggt cttttccctt gacaaacgga tcaaacatgg
     1561 aatcccggat ttcgtcggga atgccgggcc cggtatcgct gatcacgatc tggaacatac
     1621 cggtaccctg gtcatcatcc ctgataaggg ttgaaaagcg cagttcgcct tcctggccca
     1681 tcgattccac agcgttcagc accaggttca ttaccacctg cctgatcata tcgggatcgg
     1741 ccttgatttt caggttctcc tgctcatgga tctcaacaag gttgatatta ttctgcctga
     1801 agcggggttg catcaattgc agggtctctt tcaatacggg cacaatctgc acgatctgca
     1861 tctggggctt ggccgggcgg gcatatttca ggaagttctg caaaaagccc tccacccggt
     1921 tgatctcatg ggtgatgatc tccaggtcgt gcagtttgtc attgctgata ccgggttcct
     1981 gcatcatgga atagatcagc attttgatcg ccgtcaacgg gttgcggatt tcgtgggcca
     2041 gtgccggcgc tatctttccg atggcagcca gcttgctcga gcgctccagc agctccctgt
     2101 ttttccttaa gtcttcattg gcctggttga tcctgctgat gagccggctg atatggacat
     2161 ccagttcttc cagctcttta cccggcgaca ttttaatatg ttccaccacc tcgcttccgg
     2221 ccgcatccct gaccttcagc accagcttat agattgggtt caggatgatg cgcgcaatca
     2281 tccagcccag gacgattccc aggaatatcc cgccaaaacc gagcgcatac atcgcggagc
     2341 ggatgatgtt ctcattcttt tcaatgctgg tctcgtattc cagttgggca ctttcattct
     2401 taataataaa tgtatttgtc ttttcctcaa tggtattgat aaggtcctgg ctggcgtgaa
     2461 cgatcagttt gttgggctgg gagatgcggc cgatcttgtg cagttccagt gcgttggtca
     2521 gcgtttgctg atagttcgaa aacagggccg atatctgctg gatgagcgtt ttctcctctt
     2581 ccgtattggc cgattctttg gctttctgaa ggagaaggat gaaatcggtc tgtttttgct
     2641 gcaggatggc gatccgttcc gggctcctgt cgaagaggta ggtcagcgaa gccgcgcgga
     2701 tatcgtacaa tgataaggtc agctcctttg ctgttttggc gcttctgaca ttctggtcca
     2761 tcagcgccgt ggtctcgtcc tggatccggt aaatgtaaga gatgatcagg aaaccgcctg
     2821 ccaggatgat cgcaatggtc accaatgacc agacgatcat ctggtagctg aaatggaatc
     2881 tcatcctggg ccgacgcata gatggaaagt tttagtaaaa ataacaaagt tttgtgatga
     2941 aaattccaaa ttccaaagtt ccaggttcca ggttccaggt tccaattaac acccttctga
     3001 ccaatgacgc gagcgcagcg agctaatgac cccttaccac cctcatccac cgtggcaccg
     3061 aatcggccag gatgatcacc agcgacgcca tgatgatgac cgtgagcgcc aggttgaccg
     3121 atccctggat ccgcgttgag gcttccatga tctgagggat gaaaaggttt ttgatgttga
     3181 gaaatcctgc cgtcatcgtg gtgacgccta caaatgacat cggtacgatc gtgatccagg
     3241 cgtattttgc tttcccgcgg tttatcaggt aagaagtgcc gatgcaaagg gcgatggtcc
     3301 ccagcaactg gttcgccgtc ccgaacatag gccagatcgt gctgacgctc ccggtgtaga
     3361 cgaaataggc ccaggcgaac acgatgatca ggctggtgat caggttgccg ggcagccagc
     3421 tggttttacc gaacggtttg tatatcttcc cgagcgcctc ctggagcaca aaccgtgcga
     3481 tcctcgtgcc ggcatcgatg atggtcagga tgaaaagggc ctcgaacatg atcgcaaagt
     3541 ggtaccagta cgacatcagc ttctccagcc cggggatggc ggaaaagatc tgtgccatcc
     3601 cgactgccag cgagacagcc ccgccggtgc ggccggcaat gttctcaccg acctccctcg
     3661 acagcgtttc caggttcgac tgcgtaaatc ccatttcctg cagcatgggc aatatctggg
     3721 caaatttttc taccggcacg ttgatctgga aataatcgag cgggaagagg ctgctggcgg
     3781 ctatcagcgc catgacgctc actgcccctt ccgacagcat cgagccgacg gctatgaatt
     3841 tgatatgcga ctctttcatc agcattttcg gagtggtccc cgaactgaca atgctatgaa
     3901 atcccgaaat ggcgccacag gcgatagtta taaaaaggta agggaacagc ggcccgggaa
     3961 tgatggggcc tccgccgtgg atgaatttag tgaatgccgg catgtgcatc tccggcatca
     4021 cgatgatgat cccgaccgcc agcatggcca ctacggccag cttcatgatc gagctgaggt
     4081 agtcgcgggg cgacagaagc acccagaccg ggaggaccga ggcaaagaac ccgtaaaccg
     4141 cgatcaggat cgtgagggtc ggtttatcga acacgaacca gtgggctacg gccgaatcgg
     4201 ggatgtgctt tccgtaaatg acggccaggc tgagcaggat caccccgatg accgtcgcct
     4261 cgcccgtttt ccctttcctg aacctgaaca tccagaaccc catgaacacg gctattggta
     4321 ttgtcattgc aatggtaaag gttccccagg agctttcggc aagggcattg accaccacca
     4381 cgcccatgcc ggccagcgcc accacaagga tgatcaggat ggccagcgag gcaatgcccc
     4441 cgctgagttt gctcacctcc ttccgggcga tctcggccag cgatttaccg tcgtgattga
     4501 ccgaagccac caggatcacc aggtcgtgca ccgcgccggc gatcaccgag cccaggacca
     4561 tccagagaaa tcccggaaaa tagccgaact gggcggccag aacaggtccg accaatggcc
     4621 ctgccccggc aatagcggca aaatggtgac cgaacaagac ccatttcgac atcgggtaat
     4681 agttctggcc gtcgtagagg cggtgggcgg gcgtttgacg aagatcattg atagtcagcg
     4741 ccctggccat gatccagctg tagtagaacc ggtaagccag gacgaaaaag gcaattgcgc
     4801 caatgattaa cggagcggca ttcatcgctt ttattcaaat tttaaaacat tgaccggatt
     4861 tgtatttgca agcttcttta cctgaaaata aattgtaatc catgctaaaa ttaggacaaa
     4921 aatcaccggt aaaataaata tcatgggatt tatcatggtc cggtaagcaa aattcccgag
     4981 ccatttatcc atcaaaagcc atgctaacgg aagagctatc actcctgcta tgatcaccag
     5041 gcggaggaat tcgaaggtga cccggcgtgc gatcaccgga gtttcggcac ccagtacctt
     5101 tcggatgccg atctctttgc gcctcagact gaaagtaaaa ttggccagtc ccattaaacc
     5161 caggccggca ataataattg aaaggaagga aaaccacaat atggccgtag ttgcgtgccg
     5221 tactccgttg tatgaattcc tgatgaaatc actctggaag aaataatcga agggctcacc
     5281 ggttagttcg gaattccagg ccgattccac ccggcgtatg gcttcctccc ggttacccgg
     5341 atcaatcctg acggatataa aattatagcc gatccacggt tgtttggtca ggatgagcgg
     5401 ttgcaagggc tgatgaaggc tggcaaagtg aaaatcaccg acaacaccga tcactttatg
     5461 tggcccgtcg cggtagatgg tcttccccac cggatcgccc cagcccagct ttttagccag
     5521 ggtttcgttg ataatatatg aggcgctgtc ggaaccggat gctttatcaa agcctctccc
     5581 ttgtattaac gggatattca tgaccccgag gtaatctgcg tcgatgtcaa gcacattgat
     5641 catgaccggt tctttgaatc cttccgggaa atagccgttc atggtaaatc cctcaccggg
     5701 cacctcggag gatgcccctg cggccctgac cggcggcagg ctgttgaaag cgtttatcaa
     5761 agcccggctg cctaatttgg cctcttcgct aaccagtggg atcaccatta catcctcttt
     5821 atcgaatccc agcggcaagg aattcatgaa gcgcatctgc gtgaaaacag ccagggtcat
     5881 gatgaggagt atcagcgaga ccaggtattg aaaaacaacc aggatatttc tcagcgatga
     5941 ttttccttta cccgaaatca ttgtcccttt tatactcatc agaggaggaa aacgggaaat
     6001 gaaccaggca gggtagctcc ctgccagcaa gctggtcacg ataagcaaaa ggataattcc
     6061 accggccatt ttcaatccgg gagatcctgt cagcgagaga tcttttccag taaggctatt
     6121 gaactcaggt tgcaggaaat cggccaggat cagggcaatg atgaaagcgg ccccgctgat
     6181 catcatggtt tcgaaaagaa actgaaagat gatggtcatg cggctggccc cggaaacctt
     6241 tcgcatgcct atctcacggg aacgcatgaa ggacctggct gtggtcaggt tcatgaagtg
     6301 gacgcaggcg atgatcagta taaaaagggc gatactggaa aagatgtaca aggcttgcaa
     6361 atccccttct ccttccaggc ctaaatcctt tcctgaaaac aggtgaatcc gtttaagcgg
     6421 ctgaaggtca agatgaagaa taaaaccaaa tgaacggtat ttgtaattga tgtttttctc
     6481 cataaaatcc gggaatttct cttttaaaga tgaaagttca cttccagggt aaaggaggac
     6541 ataggtagaa taattccagc ccccgtccca atccaggtat aaacttttat cctgataaag
     6601 ggtagagaag gagatcaggg catcgaaatc aagatgcgaa gtggaaggaa agtccttcat
     6661 gacaccggat accataagat tctccttgtt attaagccgg acgacctgtc tgagcgggtc
     6721 cgaatcaccg aagattcttc gggcggccga ttcggtgagg acgacctgga acggctctgc
     6781 cagtgccttt ttgggatcac cccgcaacag tgaaaaagaa aacacctcaa agagcgacga
     6841 atcggcataa ctcagtttcg ccaaaggaaa atttctttcg tcggttgcca ggaaacatcc
     6901 tgaaggattg gcaaaacgga caaatttgtc gacttcacgg aattcttcag ccatcgacgg
     6961 gccgatggct gctgtaataa ttggtgtggg cgaaatctca gcttcttttt cctctttgat
     7021 gatcacccgg taaatgtgct tttgattggc atgaaaacga tcgtagctga tctcgtcgaa
     7081 tacatattcc atgatcagga taacggttgt aaggccgatg gaaaggccgg ctacatttaa
     7141 gagcgttgct gctctgtttc tccggaaaat gcggaaaccg gtgataagaa agtgctttag
     7201 cataatagcg attttcttcc ggccaccagg ccgtcggtca ataaaaatta catcggcatc
     7261 tccggggccc tggtaaactc cgccggaaca gtccctgtta atgttttcag aaatgttacg
     7321 atatccttca cctcatcttc ggtaagatct ctgttaagtt gaagttttgc gataattttt
     7381 accgcttcac ccaggtcggt cacactgccg tcgtggaaga aggggtgtgt tttttcaatg
     7441 ttccttaaag agggtacctt aaaaataaac ttatccgatt cgcttttagt gaccaggtac
     7501 cttccattgt ctaccctgga gctttgggta aaatcccagt aattgcccgc cagtccgaac
     7561 ttctggtaca tctgcccacc caggacggtt cccgagtgac aagccgtaca ccccacatcc
     7621 ataaacgtct gcaatcctct cttttcatcg gttgtcaggg cttctgtatt gcctttcagg
     7681 tattcatcaa agcgggaagg agtgatcagt ttccgttcaa aggccccgat cgctttttgc
     7741 aggttgatat agttaaccgg ttgttcttca ccgggaaacg cggcagcaaa caatttccgg
     7801 taaccttcca cagcctggag gcgtttcaat actgcttcct ggtcgggcat agccatctct
     7861 accgggttca ggatcgggcc accggcctgt tcttccacat ctttcgccct tccgtcccag
     7921 aattgactga aatggtaggc ggcgttcagc accgtcggtg agttccgttc acccttctgg
     7981 cctccgacgc caccggatgt cgcttcatta tcaactccat acgtctgcag atcgtggcag
     8041 gaattgcagc tcagggtttc atctttcgat aagcgtttgt caaaatacaa cgtctgaccc
     8101 agaataacct tttcttttgt gatcgggtta tccgggttat ccaccggttc cgtgatcggg
     8161 ctgaaggaag cttttgcttt atccagtaac ccgggaaagg catcgggtga aggtttctgt
     8221 ggcgatgggc cgcaaccggt caggccggcc atcaacaatg ccatgaatcc cattataagt
     8281 ctcatcgatt tggtttttaa tattttaaaa ggactctaca ttgatccata ttatttgaag
     8341 taaatatacc tttttcgtag aaatatccga atgccgtatt gaaaatatgc ggatcaggca
     8401 gcaggtttcc ggaacggatg cgcctccgtg gaaaaaacgc actcttttgg attaatcccc
     8461 ggtggattgg caaagatcag gtataaatac ttcagggtct cagctaaaaa gaaagtttcc
     8521 atatgatccg cctgttcttt ggtgcgcaca tcttttatat tcgtgaaagc cacgtcagtc
     8581 ctgcaataag ccattatatc ataccaatat tgagatgcca tctcaaaata cttcttttca
     8641 cccgtaaaat accagagata ataagccgat tcgatgattt ccgggtttaa attataggcc
     8701 ggatgaagga tctccttttt cccgtaatca taaaccatag gctccaggct atacttaagc
     8761 cacaggctgt gccacgaatc ctggtagcgg gcagcatttt tcagatcacc ggataagaca
     8821 aggactgctg gaaaataagc atcataaagg gtaaccaccc ggtttaccat ctctccggtc
     8881 tctttgttta cctgcccata ccagagtgaa gagtccggtt cttccgcaac gaatttgcgg
     8941 atcgcaacca ggctctcttc ccaggctctc ttcaaatcct catctttaaa caataaccaa
     9001 cctttaagaa gatactcata gtagctgtcg atgcaacacc cgatgtgact gacgctatct
     9061 acccactgcc cggtttggac attgatattc tcggcaacca gtccaagtga tgacctgcgg
     9121 gcatgaatcg cgaggctggc tttttttgct gcatcgaaaa aaacggggtt tccggtaaaa
     9181 cggctcagca tccccatctc gatcaggctc gagccgcctt cggcaaggtt gaccgtatcg
     9241 cctttgatct ctccggtccg gaggttaacc cagtatgccg ggatgccggt tccggtattg
     9301 tatgctgcta acagcctctt cccgaaatct tccgcttttg ccagcacctt cggatctttg
     9361 gtaaactggt acatggcaac aagtccgcca agaacgcgga tattgacttc gaaagtcttg
     9421 acaaactggt ctttgtcgaa attgatggta tctgccacat atttctgaac ctgggcagcc
     9481 tcctcatcca ggcccatgac cttcatggta ctgtaagcat cgatgggcga gatgccgagg
     9541 gaattctcat accagtccat gtggcttttt gaaagcggga gaagcacgtc gtggccccag
     9601 gcatatgttt tataggcttt ccatgaacgg aggaactccg atttgacctg gtctgctatt
     9661 ttttgatcgt cggttttgac gacagttgac tgacagccgg taaaagccaa agtcagaatg
     9721 aacgtaatta ttcttaaagt cctcataatt aatatattct taacctgcta ctttcaaccc
     9781 gacaattccc gttatgataa ataccaccga aacgatcctc aatgcagtaa caggatcttt
     9841 gaacaggatg ataccggtta caaaagctcc gacggcacct atgccggtcc agacggcata
     9901 agccgtgccc atcgggattg ttttctgggc catccagagg aataaaccac tcaagcccat
     9961 agcggctatg gaaaataata tccagccaaa tttatagggc gtcgtctgcg ataacttgaa
    10021 gccaaggggc cagccgatct cgaaagcacc ggcaatgatc agaaatatcc agttcatgta
    10081 attattaatt tttaattctt aattcttaat tcttaattct taaagaggat taaagattgt
    10141 aaactgtaga ctgtaaattc tagattaaat agggaatcca cagtcgtgtt ctcttcatat
    10201 actccctata ttcctccccg aacctggcaa tcatctcttt ttcttcaacc ctgcatgtga
    10261 ggtaaacggc cagcgtgatc ccggcagtga ttaaagcagt caccatattg atctctttca
    10321 ggaacatgcc ccagcccata aaaagaagcg aagcgtacat cgggtgccgg atgaagcggt
    10381 acaatcccgt tgtgaccagc cgggtggtgt tctcgaaatt ttcacccggc tttccctttt
    10441 taataaatat cataatgcca taaaatgcat aaaatataga tgaaatgaat aaaagccacg
    10501 aaatgatctg cctgggtact aatggatcat gaaaccaggt atcggcctgc aacaaaccaa
    10561 ggatcagcaa tccttcaaaa acgaaaaatc ggggaatgcc atgataacgc ttttcccgta
    10621 aggaaacgaa ccaggagaac aggatgagaa ggatggttcc tgaaatcaga atgatccatt
    10681 gggttgttga cataggaact tgtttttggt actattgata ttcgtcgatt tcgtgttatc
    10741 cgtgtgcttt ttcagaacgc aaatgacacg aatattacga atattcacga atctgatttt
    10801 ctttttaaaa aatacactac tgagcttgat tttttcaaac tgaacaggct ttccaggtaa
    10861 gccagtttgc cgatgatcgt ccctttgatc ataaaaaacc ggctcttcca gtatttctcg
    10921 ctcagcatcg agttataaaa cggatcgagc gggagccttt tcatcttaat aattttaaga
    10981 cgatgcttat cggccaggac tttgaatgtt gatggcgtaa aatgccagag atggcgcggt
    11041 acatcgtagc cggcccagta ttctttgtag tgcctggcat ccggcgaact gcaattggga
    11101 agagcaatca caagtaaccc gccgggcagc agatactcca gcatccggtc gatatacaga
    11161 tcgaaatcat cgagatgctc caggacgtgc cagagggtga taatatcgaa atatccctgg
    11221 atttcacggt tgaggaactc agccggtgtt gccacattaa ggccaaaggt cttacgggca
    11281 aactgtgcag cggccggatc cacctcaacc ccgttcacgc cgaagcctct gcgttgcatg
    11341 taagccggga aatagcccgt gccgcagccg atatcgagca gccggtcgcc ttctgactcg
    11401 tttctgacca tcctgaactt caggtacagc atgattgacc gggctgtatg gtacagccgg
    11461 ttcatcactc ccttccgggt atcggaatgg gaaacatatt catcgctttt gtaatacttt
    11521 ccgatctcat ttggcgccgg aacacctgcc gtataaacaa acccgcaatt gctgcacttc
    11581 aggagcgaaa agctctcacc cgaaaggaaa aaatccctaa cacggatata atcctttcct
    11641 gttcctgaat ggcaaagcgg acagctttta ttctctgaat ccatcattca ataaataaaa
    11701 aacacaaccc gccccaaagt taacaatgaa tatccctgga gcaacctttt atgcaatttc
    11761 ttgtatctgt cattattttt cctatcttag aacccggatt aggaacccaa taaccccaaa
    11821 tccttaatca taaatccctt ttataattaa gcactaagca ctaaaattca aacatttaac
    11881 agctatgaat ttccctaaaa aagtaactat cggcgacata accgtacgtg acggtttcca
    11941 gcatgaagag aagttcattc ctacggaagc gaagctttgg cttgctgaag agctgctcct
    12001 ctgcggcttt aaacatattg aagtgaccaa ctacggaaat ccgaaaggga tgccccagtt
    12061 caaagatgcc gatgaactga tgaaagcact ccgaacaagc aagaaagtgg cccatctgct
    12121 cggggaggtc aaactgacca ccatcaccat ccgcgagaag gcgatcgaaa gagcgatcca
    12181 ggcccgaaaa gagggttacg gccccgaccg gatcctcctg atggtttcca ccagcgaatc
    12241 gcatcaccgc acgaactcgg gcatttcaat cgaggaatat ttccggatgg cagaaaaata
    12301 tatcccgatg gccctcgacg cagggttgaa ggtgaacggc accgtcagca ccatctgggg
    12361 atgcccgatc gaagggccga ctgagatgaa aaaggctatt gaattctcca ggcgctggtt
    12421 cgacatcggc gccaccgacg tagaacacgc cgaccacgac gggtctgcct cccccgatcg
    12481 cgtttaccgc tattattcga tgcttctgga tgcttttggc tcgcccgaca agcacatcgt
    12541 acactttcat accacccgcg gctgggggct ggccaatgtg ctggcagcgc tgcaggccgg
    12601 gatgaccaac tatgaatcga ccctgggagg gatcggcggg cagccggcta atttcgtcga
    12661 tggcgttccg gtgcccggaa ccggcgaata ttactacaaa gacccgatga tcgtcggact
    12721 cgtcagcacc gaagatatgg tcgtgatgat ggatgagatg gggatcgata cggggctgga
    12781 tatcgaccgg ttgctcgaag cgggtaaaat ggcggaaagg atcgttggcc gcaggctgcg
    12841 gtcggagaca atacgcaacg gcagaatccc caagcacatg aaataagttt gaatgttaaa
    12901 ggttgaatgt tgaatgcggg aatattatct tgaattttcc tgagctgttt tgacgatctt
    12961 cgttaaaata tttataatct gatcaacttc attcatcagc gcggaaaaat cataaccctc
    13021 gatgaaatta tcttccttta aaagtttaag ccaatacctc gcttctcttg cttctttaga
    13081 agaaatactc atctttaatg caaaatcctt gcgactggaa gcagcacccg cctcttcgat
    13141 atttgcccca atgcttgtgg cgcaacgtag aagttgcctg gaaataatga actccttttg
    13201 tttcattaat tctttataaa gctgaagtac atttagcgaa aaagtgaacg atttaatgac
    13261 aataatgttt tctttcatag gataatgatt taattttatc cattaatccg gtttttaggc
    13321 taaaaatacc cggttgttaa taaaaaaatc ccgaattcaa cattcaacct tcaacattca
    13381 acattgataa caattgtcga aacaccccgc gacgctttcc aggggataaa acagttcatt
    13441 cccacggaaa ggaagatcag gatcatcgac ctgatgatga aagccggttt tgatacggtc
    13501 gaggccggca gctacgtatc cgaaaaagtg atcccgcaga tggccgatac cactcaggtt
    13561 ctgcgcggac tggataaaaa aaacagcaga tctcgggtca tggtgctggt cgtgaacaaa
    13621 cgcggcgcca tgag
//
LOCUS       DAOPNH010000053        13427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4686_length_13427_cov_6.299432, whole genome shotgun sequence.
ACCESSION   DAOPNH010000053 DAOPNH010000000
VERSION     DAOPNH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4686_length_13427_cov_6.299432"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..662)
                     /locus_tag="PKZ74_04325"
     CDS             complement(138..662)
                     /locus_tag="PKZ74_04325"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HPM92015.1"
                     /translation="MQNISLISYGYFDKALLDRIAENISQEFQSQVELREGHLDLSLY
                     YDPGRRQYNGTRLLEVVDKLPLESSYKILALFNVDLFIPILTYIFGQAYLGGKSGIVS
                     SYRLRNELYGLKQDDKLLAGRIIKVCLHELGHMHGLVHCSQPQCIMRSSTYVEDIDLK
                     ERYFCGECNAKLCE"
     gene            complement(679..2019)
                     /locus_tag="PKZ74_04330"
     CDS             complement(679..2019)
                     /locus_tag="PKZ74_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM92016.1"
                     /translation="MTGKISILIVDDEESVRDSLYNWFIEDGYHVECAANAKEALNLI
                     EQKEFSIVLADLKMPGMDGLEMQRRIKALNPEAIVIIMTAFATVDSAVRALKDGAFDY
                     ITKPFDPDDLSHLIRNASKQIMLKAENDALRDKIVSLENIDDIIGTSPAILQVLKEVE
                     SVAQSNASVIITGESGTGKELIARAIHANSSRRYFPLVSVHCGALTESLLESELFGHE
                     KGAFTGAMYNRKGRFEMADGGTIFLDEIATVSMKMQIELLRVLETRSFMRVGGNREIS
                     SDFRVICATNRDLKAMVGNGTFREDLYYRLNVVGIHVPPLRERPEDIPLLAEYFIKKY
                     CRSMSRDVIPIEPAALKRLQEFDFPGNVRELENMIERAIVVGNGKEIRVKDLPLGREV
                     STASTAESLEEQEKKHIVQILTRYDWNISRAAKALQIDRVTLYNKIRKYDLNQE"
     gene            complement(2009..3586)
                     /locus_tag="PKZ74_04335"
     CDS             complement(2009..3586)
                     /locus_tag="PKZ74_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92017.1"
                     /translation="MSVILFASFSIIFRSVNHKYLNSVIVQNGHNVGSIVKGALYQSM
                     LANDETSLQQTLDAIHSISGIDEVNLYNNRNDLIYSVFSSEIADRGNPDCITCHEDFS
                     GMFSKTEISYKIIDKKSLCTMNQTIKGYRQLLISSPILNEKTCYTADCHAHQANETFL
                     GSLLIKVPLTELDSTVSKSSARFVFFSILSTLIMATILIIFTRKRIKKPLNEIILASE
                     AVGKGNTSVRLEIDKNELDDIKVVSKAFNDMLDKIQASTKELQNWSHQLEYKIQKKSE
                     ELLEAQNELIQIEKIASLGKLSSSVAHEINNPLAGVLTYTKLVSKQLGKADMDENARQ
                     SIQKHLKVIETETKRCGDIVKGLLDFSRKDQENFSNRHLNEVLQETHNLMAHQMKVEN
                     IQFTTDFAASNDVVSCVENQIKQACIAILVNASEAVSENGDVAVRTFNPDGEHIRIEI
                     TDNGTGIAPDDIPHIFEPFFSAKNKSSGIGLGLSIAHGIVLNHKGRIEVDSRLGSGTK
                     ISIILPLANLEGKDHDR"
     gene            complement(3766..4485)
                     /locus_tag="PKZ74_04340"
     CDS             complement(3766..4485)
                     /locus_tag="PKZ74_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92018.1"
                     /translation="MDGFTYHDIFQTKGIEYIIIILFLLLLIPFWIIINKNDYLKTKI
                     KKAVRFLTRDTLRVPRGVFISPMHTWAFMEKSGMAKIGPDDLLALLAGEVNLEFLKKP
                     GEEVKRGDLIAEVGVGEKKLQIISPVSGTISRPNHSLAEQRHLFADDPYGQGWIYEIS
                     PSAWKEETNSYRLGEEAVSWTYGELDRFKDFLAKSMVRHSLAGKGIILQEGGEIMAGS
                     LAEMPEQIWVEFQEEFLSDHH"
     gene            complement(4522..5760)
                     /gene="nrfD"
                     /locus_tag="PKZ74_04345"
     CDS             complement(4522..5760)
                     /gene="nrfD"
                     /locus_tag="PKZ74_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPM92019.1"
                     /translation="MKRAMIKGEYTLEADRQKEGFWKFILKEMKPRGKIFTPFNIISL
                     PIMLTGLVLIVIRFWKGIGSVTNLTQEVPWGLWIGFDVVTGVAFAGGAYVLTFMVYIL
                     NMKKYHSIVRVTVLNGFLAYVFYAGALLLDLGRPWNVINPIIGNDFGYSSVLFLVAWH
                     FLLYMLAQLIEFSPAIAEWLGARRAHRILSGMTIAAVIFGITLSTLHQSGLGALYLMA
                     KEKIHPLWYNEFIPIMFFVSSIFAGLSLVIFEGSISHKVFFHQISEPNRKAHHGIVIG
                     LSKICAVAMFAYFFLQILIFVHGQRWEYLGTPMGYWYLVEMIGFVLFPMLLFFTSYRR
                     HNALLVKIAAIITILGIIINRLNVTVIGFKWNLEPHYFPSWMEIVVTLAVIFTEIWIF
                     RWIINRMPVLRDSPEWAKNH"
     gene            complement(5741..6487)
                     /locus_tag="PKZ74_04350"
     CDS             complement(5741..6487)
                     /locus_tag="PKZ74_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM92020.1"
                     /translation="MLYDASRCVGCQSCEYACAAAHNLPEPADSPEAGIIRKASETQQ
                     NVINLYNTSKGEVYVKQQCMHCNSPACASACLTNAMHKTHTGPVIWRGNKCMGCRYCM
                     VSCPFDIPKFEYHSPNPEIRKCDMCFDRLKEGLMPACVTECPAEALMFGTRREMIKEA
                     RRRIHDNPDVYYDHIYGEHEAGGTGFLYLSPVPFEELGFNTSIQKSSYPELSKGFLYS
                     VPTIFVLWPAILLGIREATKDKPLNEESHD"
     gene            complement(6660..8327)
                     /locus_tag="PKZ74_04355"
     CDS             complement(6660..8327)
                     /locus_tag="PKZ74_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92021.1"
                     /translation="MKRVILFLTGILLLQLSLVAAVNSGNNEEKAANDNTIRVIVSPE
                     MRELAGSLAHDFTRMQGGADVEILPVENGDFQIITDKSADFAIIPGKVPEGLGNEWVM
                     AIARSIVVPVINRGNPFLDKIYGKGITRMELAGLLTGNKPGWGQLIPGESKPAHVYVI
                     SDQSAMNSLAQYTGVAASSFRNENSAGADDVIAAVAKDPLAIGFCRLSDLKGSALAGQ
                     VTFMPIDRNENGKLDYIEMIYHDAGSFERGVWIGKYPGELYSEVYLVALKPAGGAISS
                     AFARYLVTAGQDVAEKAGFSGLVAGERLAKAERLINRQSPDDPSGNIFHSNVALIVIL
                     SFLFVALLVSALLFFAKKGGVQPSPEKIARGQLNPESIGIPQGLLFSKSHTWSFMDRD
                     GKVKIGIDDFMQHIVGPVTRVEMKKAGEKIRKGDILVKLVQNGKQLHLYSPVSGTIAE
                     FNPLLKSDPSQLNASPYTSGWLYRVEPANWMKELQFMEMADSYRKWISDEFSRLRDFL
                     ATSIKVSRPELQYVVLQDGGHLKDHVLADFGPEVWEDFQTSFLDKAR"
     gene            complement(8614..10719)
                     /locus_tag="PKZ74_04360"
     CDS             complement(8614..10719)
                     /locus_tag="PKZ74_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPM92022.1"
                     /translation="MKFRIFSLSIMMVFAVVTVSSKPVNIQTARITAGNFLRQTERLY
                     GYSPAGSYVIDDKPFVQRNDGLPVFYLFNLDPGFILISGDDAYTPVIGYSFESGLDLE
                     NAPAHVKGFIDNYAAQIAFIRDRQIPPSEEIQNEWADLLSGTTGEGAGLRTSREVEPL
                     LFCKWDQGAPYNMLCPEDEAGPGGHVWVGCVATAMAQVMYYWRYPAAGTGSHCYWPGN
                     WQYGQQCADFGNTYYNWEGMINSIDLVQVYPNAELQYHCAVSVDMDFSPNGSGSYSHM
                     VPERLAAFWGYSDAQILWKDDYSHSEWVEILQEELDASHPMYYSGYSNEGGHAFVCDG
                     YQGEYFHFNFGWGGSANGYFTLDNVGGFSYGQACIRYFVPSDPAYPYYIDGDHIMTSH
                     SGSFTDGSGPVGNYTVNQNATWLIDLGDSGDSIAGISLSFSMFDLGAGDTVTIYNGTT
                     RTDPILGAYTGTSVPQQINSTGDAMLVAFSSDSQVTGGGFYAEYYANSAVFCAGMTTM
                     TDMEGSMNDGSGSYNYHSNSYCTWYIRPEEAGTITLTFHSFDTESGVDKVTVFDGNVK
                     VGEFSGQEVPEPVVAQNGSIFMTWLTDGDNTGQGWSASYEAEAVGITEDPSASSINIY
                     PNPASGKINIEIKADREEEMEIRLVTLLGVDKYKGMISVRPGTNRITISVEDIPAGQY
                     FVELGSPLQKTVRQIAVIH"
     gene            10823..11473
                     /locus_tag="PKZ74_04365"
     CDS             10823..11473
                     /locus_tag="PKZ74_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HPM92023.1"
                     /translation="MPQQKKESKTLARMIGSLSGNPTEIKKESLRLLASYREKYGDKL
                     TEEEIRRKAANKIISNYSYYSAFTGGATSLTGIVPGIGTAIAALGGVSADVVLSMKFQ
                     IDMVMAIAAVYGHDIEEEYVQQTSFLVAGIGKLNFAVDEGKRIGAKAFANIMNQYLKK
                     GSQQAAKEITKKLGIQVSGKALQKAIPFGVGVIIGFTANKTLTWYIGRKARDYFAG"
     gene            11550..12227
                     /locus_tag="PKZ74_04370"
     CDS             11550..12227
                     /locus_tag="PKZ74_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92024.1"
                     /translation="MRILVIEDEPKVAHFIKEGLEGQKHEVDMAYDGLTGESIALQNT
                     YNLIILDIIIPYINGIELCRRIKSQKPRVPVMMLTALGTTDDKVTGFEAGADDYLVKP
                     FEFRELIARVNALSKRPLEASGPPNCLKSADLELDLDKKVARRGGKTIELTAKEFSLL
                     EYFMINKGRVVSRVDIAEKVWDINFDTGTNVVDVYVNILRKKIDKEYEPKLIHTRIGM
                     GYIFGEE"
     gene            12229..>13427
                     /locus_tag="PKZ74_04375"
     CDS             12229..>13427
                     /locus_tag="PKZ74_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPM92025.1"
                     /translation="MQIRARLTLQFITIVALTLLLAALAIYYFSEGHRKEDFFDRLNK
                     KASNTAMLLIQFDEVDINLLKKMEADNPVSLPREKIIIYDYKDNIIYSTDEVSLFTID
                     KALLDNIRLNEEVRLKQGEYEILGFLFTHQYDRFVVIAGAVDIYGLKRMHNLRNILII
                     VVSISILLMLVSGWIYAGRALYPISRIISKGNEISITSLNLRLDEGNQKDEIAQLAGT
                     FNKMLDRLETAFKSQKRFIANASHELRTPLTAITGQLEVLLMQDRTTEEYKRAVNSVL
                     EDMKNLNSISNRLLLLAQASNDLSELNVKPIRIDETIWQCREELIRRNENNHIIVHLD
                     NSLEDDAALTFMGDEQLVRTMIINLMDNGCKYSPDHTVEVTIGADQLQLLLTFTDRGI
                     GIPEEDLA"
BASE COUNT         3518 a         3461 c         3014 g         3434 t
ORIGIN      
        1 aaagccgtcg gttccatctt tgtaatattc ttggaaccaa cggctttagt cgttgaagca
       61 cgaattgttt tcaggcgatc aaatcgaaat atcagacttt tgtgacagtc tcagcaatac
      121 taaggaatca ggggaactta ttcgcataat ttagcgttgc attccccgca gaagtacctc
      181 tctttcaaat caatatcctc cacataggtg ctcgaacgca tgatgcactg cggctggctg
      241 cagtgcacca gcccgtgcat atgccccagt tcatgcaaac aaactttgat gatccggccg
      301 gccaggagct tatcatcctg cttcaggccg tatagctcat tccttaaacg ataagaggat
      361 acgatgccgg acttgcctcc caggtaagcc tggccgaaaa tataggtcag aatggggatg
      421 aacaagtcaa cattgaacaa ggcaaggatc ttatacgagg attccagggg aagcttatcc
      481 acaacctcca gtaaacgggt cccgttatat tgcctcctcc ccggatcata atacaggctc
      541 aggtcaaggt gcccctcccg gagttccacc tggctttgaa attcctgcga aatgttctct
      601 gcaatcctgt caagtaaggc tttatcaaaa taaccgtatg atatcaggct gatattctgc
      661 acgttgctgg ttaccgcttt actcctggtt taaatcatat ttcctgattt tgttgtaaag
      721 tgtcaccctg tcgatctgaa gggctttggc tgcgcgcgaa atattccagt cgtaacgtgt
      781 cagaatctgt acaatgtgtt tcttctcctg ctcttcgagg ctctcggcag tgctggccgt
      841 ggaaacttcc cggcccaggg gcagatcttt tacccgtatc tcttttccgt tacccaccac
      901 aatagctctt tcgatcatat tctccagctc cctcacattc cccggaaaat caaactcctg
      961 caaacgcttc agggctgccg gttcaatcgg gatcacatcg cggctcatcg accggcaata
     1021 cttctttatg aaatattccg ctaaaagagg tatatcttcc ggacgttccc gcaaaggagg
     1081 cacatggatc cccacgacat tcagcctgta ataaagatct tccctgaaag ttccgttccc
     1141 gaccatcgcc ttcagatcgc ggttcgttgc acagattacc cggaaatcac tggaaatctc
     1201 cctgttgccg cctacccgca taaaacttct ggtctccagg acccgtaaaa gctcaatctg
     1261 cattttcatc gaaacagtgg caatctcgtc gagaaagatc gtaccgccgt cagccatctc
     1321 aaaccgccct ttccggttgt acatcgcacc cgtaaaagcc cctttctcat gcccgaataa
     1381 ctcgctttcc agcaaactct ccgtcaacgc cccgcaatgc acactcacca ggggaaaata
     1441 gcgcctggat gagtttgcgt gtatggccct ggctatcaat tctttcccgg taccgctttc
     1501 gccagtgatg atgacggaag cattgctctg cgccacactc tccacttctt ttaatacctg
     1561 gagtattgcc gggctggtgc caataatatc atcgatattc tcaagagata caattttatc
     1621 tctcagggca tcattttcgg cctttagcat aatctgcttc gaagcgttgc ggatcaggtg
     1681 cgacaggtca tccgggtcaa aaggcttggt gatataatcg aatgcgccgt cttttaatgc
     1741 ccgcactgcc gaatcgacgg tagcaaatgc cgtcatgatg atgacgatcg cctccgggtt
     1801 cagggctttg atccgccgct gcatctccag cccgtccatc cccggcatct tcaaatctgc
     1861 cagcacaatg ctgaattcct tctgctcgat caggttcagc gcctctttgg cattggccgc
     1921 gcactcaaca tgatatccgt cttcgataaa ccagttgtac agcgaatccc tcaccgattc
     1981 ttcatcatcc acaatcaata ttgaaatttt accggtcatg atcttttcct tcaaggtttg
     2041 ctaaaggcag gataattgaa attttggtgc cgcttcccag ccgggaatcg acctcgatcc
     2101 gccctttatg atttaacaca atgccatgcg caatggaaag tcccagtccg atgccgctcg
     2161 atttgttctt ggctgagaaa aacggttcga aaatgtgcgg aatatcatcc ggggcaattc
     2221 cggtgccgtt gtcggtgatc tcaatccgga tatgctcccc gtcaggattg aaagtgcgga
     2281 cagccacatc gccgttttcg ctgaccgcct ccgaagcatt gaccaggata gcgatacacg
     2341 cttgcttgat ctggttctcc acgcatgata ccacatcgtt agacgccgca aaatcagtag
     2401 tgaactgaat attctcgacc ttcatctggt gggccatcag gttgtgagtc tcctgcaaaa
     2461 cttcatttag atgacggttg gagaaatttt cctggtcctt ccttgaaaaa tccagcaacc
     2521 ctttgacaat atccccgcat cgcttggttt ccgtttcaat cactttcaaa tgcttctgga
     2581 tcgactgcct ggcattctca tccatgtccg ccttccccaa ttgtttggat accagttttg
     2641 tataagtcag aactccggca agcgggttgt ttatctcgtg tgcaacagaa gacgacaatt
     2701 tccccagcga agcaatcttc tcaatctgga tcagctcgtt ctgtgcttcc agcaactcct
     2761 ccgatttttt ctggatctta tactccagct ggtgcgacca gttttgtaat tctttggtcg
     2821 acgcctggat tttatccagc atgtcattaa aagctttcga tacaactttg atatcatcca
     2881 gctcattctt gtcaatttca agcctgactg aagtgtttcc cttcccgaca gcctcgctgg
     2941 caaggatgat ctcattcagc ggcttcttga tccttttccg ggtgaaaatg atcagtatcg
     3001 tggccatgat cagggtcgaa aggatagaaa agaaaacaaa acgggctgat gatttgctga
     3061 ctgttgaatc cagttctgtc aacggaacct tgatcagcaa ggaaccgaga aaagtttcat
     3121 tcgcctggtg ggcatgacaa tctgccgtat aacaggtctt ttcgttcaga ataggggaac
     3181 tgataagcaa ttgccggtac cctttaatgg tttggttcat cgtacataaa ctcttcttgt
     3241 cgatgatctt gtaagatatc tcggttttgg aaaacatccc gctgaaatct tcatgacacg
     3301 ttatgcaatc gggatttccc ctgtcggcaa tctccgatga aaaaaccgaa tagatcaggt
     3361 cgttccggtt attataaaga ttgacctcat caatacccga tatcgagtgg atcgcatcca
     3421 gggtctgctg aagagaggtt tcatcattcg ccagcatcga ttgatacagc gcacctttta
     3481 caatcgaacc gacattgtgc ccgttctgta caataaccga attcagatat ttgtgattga
     3541 cagaacggaa aataatacta aacgaggcaa acaatatgac agacattgcg gcaattatga
     3601 tgaccacacg tgtgtatata gatgacccta ttttgattct gacgggtaaa acggacttaa
     3661 aggccccctt ttttatcatt acatcatttt tgggatgatt ttgatctcct aaattaagaa
     3721 attcttgtat cgcttaaata tttcagcgct ttatcatcct aataattaat gatgatcgct
     3781 taaaaactct tcctgaaact caacccatat ctgttcaggc atctctgcca gtgagccggc
     3841 cataatttca cctccctcct gcaggatgat ccctttgcca gcgagtgagt gacggaccat
     3901 cgactttgcc aggaaatcct tgaaacggtc cagctctccg tacgtccagc ttaccgcctc
     3961 ttcacccagc cggtaactat tcgtttcttc tttccaggcc gatggactga tctcatatat
     4021 ccacccttgc ccgtacggat catccgcaaa caaatgtctc tgttcggcga gtgaatgatt
     4081 cggccggctt atcgtacccg aaaccggaga aatgatctgc agtttttttt cgcccacacc
     4141 gacttctgcg atcaaatccc ctcttttgac ttcttccccg ggcttcttta aaaattccag
     4201 gttaacttct ccggccagaa gagccaggag atcatcagga ccaatctttg ccatgcccga
     4261 tttttccatg aatgcccagg tatgcatcgg actgatgaaa accccgcggg gcacccgcaa
     4321 tgtatccctg gtcaaaaacc tgacggcctt tttgatcttt gttttcaggt agtcattttt
     4381 attgatgatg atccagaaag ggatcagcaa cagcaggaac agaatgatga tgatatattc
     4441 tatacccttg gtctgaaaaa tatcatgata agtaaaaccg tccatcttat atttttttaa
     4501 gattaattaa tgaattgttt ctcaatgatt tttagcccat tccggggaat ccctgagaac
     4561 cggcatccgg ttgatgatcc accggaatat ccatatttcc gtgaaaatca ccgccagtgt
     4621 caccacgatc tccatccagg acgggaaata atgaggttct aaattccatt tgaaaccgat
     4681 aacagtcaca ttcaaacgat taatgataat ccccagaatg gtgataatag cggcaatctt
     4741 taccagtaag gcattgtgcc ggcgatagct ggtgaaaaac aacagcatcg ggaacagaac
     4801 gaaaccgatc atttctacca ggtaccagta tcccatgggg gttccaaggt attcccaccg
     4861 ctgcccgtgc acaaatatca ggatctgcag gaagaaataa gcaaacattg ccactgcgca
     4921 tatcttcgac aacccgatca caatgccgtg atgggcttta cggttcggct cactgatctg
     4981 gtgaaagaat accttatgac tgatggatcc ctcaaaaatg accagcgaca atcccgcaaa
     5041 gatgctgctg acaaaaaaca tgatcgggat gaattcgttg taccaaagcg ggtggatctt
     5101 ttctttcgcc atgaggtaaa gcgcaccgag acccgactgg tgcaacgtcg acagggtaat
     5161 accgaaaatg acggccgcaa tggtcatccc cgaaaggatc ctatgcgccc gcctggcccc
     5221 cagccattcg gctatggcag gcgaaaactc gatcagttgt gcaagcatgt aaagcaggaa
     5281 atgccatgcc accaggaaca ataccgaact gtacccgaag tcgttaccga tgatcggatt
     5341 gatcacgttc cagggcctgc cgagatccag cagcaatgcg ccggcataaa atacgtaagc
     5401 caggaatccg tttaaaacgg ttacccttac gattgaatgg tatttcttca tgttcaggat
     5461 gtagaccatg aaggtcagta cgtaggcgcc acctgcaaaa gccacgcccg tgaccacatc
     5521 aaacccgatc cagagccccc acgggacctc ctgcgtcagg ttcgttaccg atccgatccc
     5581 tttccagaac cggatgacga taagcaccaa tccggtcagc atgatcggaa gtgaaataat
     5641 attgaatggg gtgaaaatct tacccctggg tttcatctct ttcaggataa atttccagaa
     5701 accctctttt tgccggtcgg cttccaaagt atattcgcct ttaatcatgg ctctcttcat
     5761 ttaaaggttt atcttttgta gcttctctta ttccaagcaa aatggccggc cagagaacga
     5821 aaatggtggg gacgctgtag aggaaccctt tggataattc gggataggag gatttctgga
     5881 tggaggtatt gaaccccagt tcttcaaaag ggacgggaga aaggtagagg aatcctgtgc
     5941 cgcctgcttc atgctctccg tatatgtgat cgtagtaaac atcgggattg tcgtggatcc
     6001 tcctcctggc ttctttgatc atctctctcc gggttccgaa catcagggct tcggccgggc
     6061 attcggtaac acaggcgggc ataaggcctt ccttaagccg gtcaaagcac atgtcgcatt
     6121 tcctgatttc cggattgggg ctgtgatatt cgaatttagg aatatcaaac gggcaggaaa
     6181 ccatgcaata acggcacccc atgcatttat tgccccgcca gatcacaggc ccggtatggg
     6241 ttttatgcat ggcattcgtc aggcaggccg aagcacaggc aggcgagttg caatgcatgc
     6301 actgctgctt gacgtaaacc tcccctttcg acgtgttgta caaattgatc acattctgct
     6361 gtgtttcgct cgccttgcgg atgatccccg cttccggcga atcggcgggc tcaggaaggt
     6421 tgtgcgccgc cgcacaggca tactcgcagc tctggcatcc gacacagcgg ctggcatcgt
     6481 aaagcacccc catgaattct tccggcggcg catcttttgc tgcctctgtt gtggcggcgt
     6541 ttaatttttt cccggtggcc agcgctacgc ctgtaacccc cagtaccttg aaaaaatttc
     6601 atctgtcaat tggcataatg ggtgtaatat aatgatgaaa ctatattatt tacctgtgat
     6661 taccttgctt tgtcaaggaa agatgtctgg aaatcttccc aaacctccgg cccgaaatcg
     6721 gccaggacat gatctttcaa atgtcctcca tcctgcaaaa caacatactg caattcagga
     6781 cggcttactt tgattgaggt cgccaggaaa tccctcagcc ttgaaaattc atccgaaatc
     6841 cacttcctgt agctgtcggc catctccatg aactgaagct ctttcatcca gtttgccggc
     6901 tcaacccggt aaagccagcc cgatgtatag ggcgaggcat tcaattgcga tggatcggat
     6961 tttaaaagtg gattgaactc ggctatggtc cctgagaccg gtgaataaag atggagctgc
     7021 ttgccatttt gaaccagctt aaccaggata tcgcctttcc ggatcttctc tccggctttc
     7081 ttcatttcta cccgtgtcac aggtcctacg atgtgttgca tgaaatcatc gatcccgatt
     7141 tttaccttcc cgtcacggtc cataaaactc caggtgtgcg attttgaaaa aagcagtccc
     7201 tgcgggatac ctatggattc ggggttcagt tgcccccggg cgattttttc cggagaaggc
     7261 tgaacacctc cctttttggc aaaaaataac agggccgaaa ccagcagtgc aacaaaaaga
     7321 aacgataaaa tgacgatcaa agcgacattg gaatggaaga tgtttcctga aggatcgtcc
     7381 ggtgattggc ggttaatgag gcgttcggcc ttggccagcc tctcgcctgc gaccagtccc
     7441 gaaaaaccgg ccttctcagc cacatcctgc ccggctgtta ccaggtacct ggcaaaggcc
     7501 gacgaaatag cgccgcccgc cggtttcaaa gcaaccaggt atacctccga ataaagttct
     7561 cccggatact tgccgatcca tacgccccgc tcaaacgatc cggcgtcatg gtagatcatc
     7621 tcgatgtaat ctagcttccc gttttcattg cggtcgatag gcataaaagt gacctgcccc
     7681 gccagcgcag atcccttcag atccgaaagc cggcaaaaac cgatggccag gggatccttt
     7741 gccaccgctg cgatcacatc atcagcaccg gccgaatttt cattcctgaa tgatgatgcg
     7801 gcaactccgg tgtattgggc cagggagttc attgccgatt gatccgaaat cacatataca
     7861 tgggccggtt tactttctcc cggaatcagt tgcccccatc cgggtttgtt tccggtcagc
     7921 agaccggcca attccatgcg ggtaatacct ttgccgtaga ttttgtccag gaacggattc
     7981 ccgcgattga tgaccggcac aactatactc cgcgctatgg ccatcaccca ttcatttcca
     8041 agaccttcgg gtactttacc cggaataatg gcaaagtcgg cactcttatc cgtgattatc
     8101 tgaaaatctc cattctccac cggcagaatc tccacatccg ctcccccctg catccgggta
     8161 aagtcatggg ccaaactgcc ggccaattcc ctcatttccg gggatacgat cacccggatg
     8221 gtattatcat ttgctgcctt ttcttcgttg ttcccgctat taaccgctgc caccagggat
     8281 aactggagta acagaattcc cgttaaaaat aagatcaccc ttttcataat cgtgatgatt
     8341 aaaatgtttt tagaaaattg attgcttccg gatttcattt tacctgtttt gaaactgagc
     8401 cggtcaattt tgatgccagt aattctataa cattgattta ctatctctta tgaattcacc
     8461 ttgggaaaag ttgcggataa aaaccagtct gtagtgttga atatttaaac aacacaatta
     8521 caaatcagtt gtaatcttat gaatataaag ttattaggat aggatgtaaa gaaattcaac
     8581 agccgttgaa aaaatcaaca accggaatat aagttaatga atgacagcaa tttgccgcac
     8641 cgtcttttga agaggcgacc cgagttccac aaagtattgc ccggcaggaa tgtcttcaac
     8701 ggaaatcgtt atccggttgg ttcccggcct gaccgagatc atacctttat atttatcaac
     8761 gcccaataaa gtcaccagcc ggatctccat ttcctcttcc cggtctgctt ttatttcaat
     8821 atttatcttg ccggatgcag gattaggata aatatttatg ctgctggcac ttggatcctc
     8881 cgtaatgcca acagcctcag cctcgtagct tgcactccac ccctggcctg tattgtcgcc
     8941 atcggtcagc caggtcataa aaatgcttcc gttctgcgca acgaccggct ccgggacttc
     9001 ctggccagaa aactcaccga ctttaacatt tccgtcaaaa acggtgacct tatccactcc
     9061 gctttcggta tcgaacgaat gaaaagtcag ggttatggtt ccggcctctt cgggacggat
     9121 ataccaggta caataagagt tgctatggta attgtaactc ccgctgccgt cattcataga
     9181 tccttccata tcggtcatcg ttgtcatccc cgcacaaaaa acagcgctgt tggcataata
     9241 ctcggcataa aaccctccgc cggtaacctg gctatccgat gagaaagcca ccagcatggc
     9301 atcacccgta gaattgatct gctgtggaac cgacgtcccg gtataggccc caaggatggg
     9361 atcggtcctt gtcgttccgt tatatatcgt aaccgtgtct cctgctccaa gatcgaacat
     9421 gctgaatgag agcgaaatac cggcaattga atcacctgag tctcccaggt cgatcagcca
     9481 ggtggcattc tggttgacag tataatttcc caccggcccg cttccgtcgg taaaagaacc
     9541 gctgtgactg gtcatgatat ggtcgccgtc aatataataa ggataagccg gatcggaagg
     9601 gacgaaatag cggatacaag cctgcccgta agaaaatccc ccgacattat ccagcgtgaa
     9661 atagccattt gcactgccgc cccagccgaa attgaagtga aaatattcgc cctggtaacc
     9721 atcgcacacg aacgcatggc cgccttcatt actgtagccg gaatagtaca tcggatggct
     9781 cgcatcgagc tcctcctgca ggatctcgac ccactccgaa tgcgaatagt cgtctttcca
     9841 caggatttgt gcatcactat atccccagaa cgcagcgaga cgctccggaa ccatatgcga
     9901 ataggaaccc gaaccgttgg gactgaagtc catatcaacc gacaccgcgc agtggtattg
     9961 aagctcggca ttcggataga cctgcaccag gtcgatgctg ttgatcattc cttcccaatt
    10021 ataatacgta ttcccgaaat cggcgcactg ctggccgtac tgccagttcc cgggccagta
    10081 gcaatggctt cccgtgcctg ctgcagggta gcgccagtag tacatcacct gggccatggc
    10141 ggtagctaca caccccaccc acacgtgtcc gcccgggccg gcctcatctt ccgggcaaag
    10201 catattgtaa ggagctccct ggtcccactt gcagaaaagc agcggctcca cctcccggct
    10261 tgtccgtaaa ccggcgcctt ctccggttgt acctgacaac aaatccgccc attcattctg
    10321 aatctcttcg gatggcggta tctgccggtc gcggataaag gcgatctggg cggcatagtt
    10381 atcgatgaac cctttcacat gggccggagc attctccagg tccaggccgc tttcgaaaga
    10441 atagccgatc accggcgtat aggcgtcatc gcccgaaatg aggataaatc ccggatccag
    10501 gttgaacagg tagaaaaccg gcaatccgtc atttcgttgt acaaatggct tgtcatcgat
    10561 cacataggat ccggccgggc tatagccata gagcctttcc gtctgccgga ggaaatttcc
    10621 ggcagtgatc cgggccgtct gaatattaac cggctttgaa gaaacggtaa cgacggcaaa
    10681 caccatcata atggagaggg agaaaatcct gaatttcatt gcgcattatt tgggtttgta
    10741 aaaatagaac ttttaaagct taaaaatcat ttcttcctga tattccaatt atagcgaaat
    10801 tagctatctg aattgtcaca caatgccgca acaaaagaaa gagagtaaaa cgctggccag
    10861 gatgatcggt tccctgtcgg gcaatcccac ggaaataaag aaagaaagcc tgagactatt
    10921 ggcctcttac cgtgaaaaat acggtgataa gctgacggag gaagagatcc gccgaaaagc
    10981 ggcgaacaag atcatttcca actactctta ctactccgcc ttcaccggcg gggccacctc
    11041 tctcaccggc atcgttcccg gaataggaac agcgattgca gccctgggtg gcgtttcagc
    11101 cgacgtcgtc ctttcaatga agttccagat cgacatggtc atggccattg cggcggttta
    11161 tggccatgat attgaagaag aatacgtaca acagaccagt ttcctggtag ctggaatcgg
    11221 aaagctgaat tttgcggtgg atgaaggcaa gcggatcgga gctaaagcat tcgccaatat
    11281 catgaaccaa tacctgaaga aagggtcgca gcaggccgcc aaagagatca cgaaaaaact
    11341 ggggatacag gtttcgggga aagccctgca aaaagccatt ccgtttggcg tcggcgtcat
    11401 tatcgggttc acggctaaca aaacactcac ctggtatatc ggccggaaag caagggacta
    11461 ttttgccggg taatttcatg ttccatttgc attttgcatt tcaatttcat ggtgaaattg
    11521 cttactttta taattaaaac cttgtttgca tgcggattct tgtaattgaa gacgaaccaa
    11581 aagtagccca tttcatcaaa gaaggactgg aaggccagaa gcatgaggtg gatatggcgt
    11641 atgacgggct gacaggcgag agtatcgcct tacaaaacac ttataatctt atcatcctcg
    11701 atatcatcat accttatatc aatggtattg aattgtgcag gaggatcaaa agtcaaaaac
    11761 cccgggtccc tgtgatgatg ttaacggcat tggggactac cgatgataag gtcaccggct
    11821 tcgaagccgg ggctgacgat tacctggtca aaccttttga attccgtgaa ctgatcgccc
    11881 gggtcaatgc attgtcaaaa aggcccctgg aggcatccgg acctccgaat tgccttaaat
    11941 cggccgacct ggaactcgac ctcgataaaa aagttgcccg ccggggcggg aaaaccattg
    12001 aactgacggc aaaagaattc agcctgctgg aatatttcat gataaataaa ggcagggttg
    12061 tttcgagggt ggatatagcc gaaaaagtat gggatatcaa ttttgacaca ggcactaacg
    12121 tggtggatgt ttatgtgaat atcctccgca agaaaatcga caaagaatac gaaccaaaac
    12181 tgatccatac ccggatcgga atgggctata ttttcgggga ggaatgatat gcagatccgc
    12241 gccaggctta cgctgcaatt catcaccatc gtggccttaa cattgcttct ggccgcactg
    12301 gcaatctatt atttttccga gggtcacagg aaagaggact tttttgaccg cctgaataaa
    12361 aaagccagca ataccgcgat gttgctgatc cagttcgatg aagtggatat caacctgctg
    12421 aaaaaaatgg aagccgataa cccggtcagc ctccccaggg aaaagatcat catctatgat
    12481 tataaggata atatcatcta ttccaccgat gaagtttcat tgtttacaat agacaaagcc
    12541 cttctcgata acatccgctt aaatgaagaa gtacggctta aacaaggaga atacgaaatt
    12601 ctgggtttcc tttttacaca tcaatacgac cggtttgttg tcattgcagg cgctgtggat
    12661 atttatggcc tgaagagaat gcataacctg cgcaatatcc tcatcatcgt agtgagcatc
    12721 agcattctcc ttatgctggt gtcgggctgg atctatgcag gcagagcgct ttatcccatc
    12781 tccaggatta tttcaaaagg caacgaaatt tcaattacca gcctcaatct gcgcctggat
    12841 gaagggaatc aaaaagatga gatcgcccag ttggccggaa ccttcaataa aatgctggac
    12901 aggctggaaa cagcattcaa atcgcagaaa cggttcatag ccaatgcttc ccatgaactg
    12961 agaacgcccc tcacggccat aaccggacag cttgaagtgc ttctgatgca ggaccggacg
    13021 accgaagaat ataagcgtgc ggtcaactcc gtattagaag atatgaaaaa ccttaactct
    13081 atctcaaaca ggttattgct gttggctcag gccagcaacg acctgtcgga attgaatgtc
    13141 aaaccgatcc gaattgatga aacgatctgg cagtgccgcg aagaattgat cagaagaaat
    13201 gaaaataacc acatcatagt ccatcttgat aactccctgg aagatgatgc agctttgacc
    13261 ttcatgggcg atgaacaatt ggtcaggacc atgataatca acctgatgga caatggctgc
    13321 aaatactctc ccgatcatac ggtggaagtg acgattggag cggatcaact gcaactgctg
    13381 ctgactttca ccgaccgggg tattggtata cctgaggaag acctggc
//
LOCUS       DAOPNH010000054        13343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4732_length_13343_cov_6.321945, whole genome shotgun sequence.
ACCESSION   DAOPNH010000054 DAOPNH010000000
VERSION     DAOPNH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_13343_cov_6.321945"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKZ74_04380"
     CDS             <1..564
                     /locus_tag="PKZ74_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM92026.1"
                     /translation="QQAAHGRAPALATGIKRTWPNKVVFTYQGDGDLAAIGTAETIHA
                     CNRGENIIIFFINNGIYGMTGGQMAPTTLPNMKSSTSPYGRDTQIMGHPLKITELVAQ
                     LPGTYFVTRQAVHTPANVRKTKKAVRMAFENQLGNKPGLSFIEIVSNCNSGWKMTPVQ
                     ANQWMVDNMFPFYPLGDIKVDGALVAK"
     gene            601..1146
                     /locus_tag="PKZ74_04385"
     CDS             601..1146
                     /locus_tag="PKZ74_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPM92027.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKIMAYSGIMQDQEVSWMPSYGPEMR
                     GGTANVTVILSDERISSPILTRYDTAIILNQQSMDKFEPMVKPGGTLIYDANGITRHP
                     ERKDIKIYRVDAADEASKMGLSKIFNMIVLGGFLRIRPIISLENVIQGLKKSLPARYH
                     HLIPDNEKALKRGMEIIAQVN"
     gene            1206..3326
                     /locus_tag="PKZ74_04390"
     CDS             1206..3326
                     /locus_tag="PKZ74_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM92028.1"
                     /translation="MAPIHNRNLKIPHFHSLRVYLVSTILYFFLVLPVASILALKYYI
                     PDYLYQKSEPAAIANPDISTDRQVYDPTPDTLGKTVDSIRRAENNSLVTVRFSTPDSP
                     QFQITTGENTSENSRFGGTISVLMKLLFVSFILGLFFNLPFKIYFSKKRNLKPIKPGV
                     FQYCRRLILKVPLINTGILALPYLITLLYMLYFILFRRDEDPLDRQFYTQYFFITLVA
                     GILTLMLVFFWLKHRVHIRYLEHIYSPDELKKRIFKGKPGRIKNRLWISSAMTTLFPL
                     IIVVFYIVLSISSVEELSLLNLSDENQKILLGKYIAFDLGLDLPTLNEFFYVNAVNSF
                     LMVVGIGTGVFIALIYVVLFVRWTTEDIIYPVNELLANMQRTGRGELDIYSIVRTNDE
                     VGALTEGYNEMTGKLKSYFESLDKINRSNARFVPRQFLDFLGKENITEIQLGDQVQQE
                     MTIMFSDIRDFTSISESMTPKENFDFINNYLGYMEPVIRTNNGFIDKFMGDSIMALFP
                     ERSEDALNASIEMRIKLLEFNEIMGQFGKPAIDAGIGIHTGLLMLGVIGGEGRMDSTV
                     ISDAVNLASRLEGLTKIYGGSIIITEDTLIKLNDPNHYHFRFLDVVKVKGKKEAIYIF
                     EIMDGEPERIRNLKLMTKDDYNKALQSYKKKEFEKALNLLQNIEAVNPDDKAVKIYID
                     RCKHYLKNGVPDMWDGVASFEMKY"
     gene            3374..3964
                     /locus_tag="PKZ74_04395"
     CDS             3374..3964
                     /locus_tag="PKZ74_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM92029.1"
                     /translation="MNYEAAKKYIITRLEKELDPRLTYHSLSHTLDVLESAVRLADME
                     QLPETEKLLLMTACLYHDSGMLITYRGHEDASIMICREKLPEFEYSAEEISAVCRMIN
                     TTKLPQCANKPADKILCDADLDYLGRPDFFMISHKLKYEWDILNIHPTSLFEWYQIQK
                     DFLSGHRYFTKSAKELRQEFKMQILSQIEFMCNHHK"
     gene            4028..4867
                     /locus_tag="PKZ74_04400"
     CDS             4028..4867
                     /locus_tag="PKZ74_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPM92030.1"
                     /translation="METAKNPIFLVPTDFSEVCANAAHRAASLAKDFNYKVVLLHVID
                     RNTISELKKEGKGPEWVDEKLKDLSNALNLEYGVKVETISQEGDIFTTIAEVANDIKA
                     SLIFLGTHGKVGMQKITGSFALKVVTSSELPTIVVQKRPFGKGISTIVMPITSDAGPW
                     AKTKWAASIAKEFNSTIHIFHLPGEEMEDVITMITNHFQVNGVKFEIKEAEKATSFTK
                     QVIDYSTARNADMILIMTNPDKSLKSFLLGSYDEDIIFNTSQIPVMCINPRDFNWKKI
                     IPR"
     gene            4914..5774
                     /locus_tag="PKZ74_04405"
     CDS             4914..5774
                     /locus_tag="PKZ74_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPM92031.1"
                     /translation="MNITSDKENIILVPTDFTPVGQNAIDHGVKACEILGFKLVILHV
                     INRETRAVFGKTDTEKAVQQRLAEIAEAVKTKSTIEVEVAAREGSIFDVISEVAKELK
                     VNLMILGTHGKRGLQHLMGSYAMKVITQSPVPVIVVQKRQIGPGGYRKVVFPISLHTE
                     SRQQVFYAIVTQLLFKGEIHIFKQTGNNPEDNTKLRIITSQITEEFDNKHVRYSLTEA
                     DRASNFTGQLLDFAVSESADLIMMITDSDSIHPTFDNSSWSEKLIFNIAQIPVFCINP
                     VFLGQTYYSF"
     gene            5833..7887
                     /gene="ligA"
                     /locus_tag="PKZ74_04410"
     CDS             5833..7887
                     /gene="ligA"
                     /locus_tag="PKZ74_04410"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPM92032.1"
                     /translation="MTTAEARSRMEWLAGEINRHNYRYYVLSDPLISDYEFDMLLEEL
                     VHLENLYPELATADSPTKRVGTDLTKEFMQVDHRYPMLSLGNTYSEQEIIEFDGRVRK
                     LLDNEAPEYVCELKYDGVAIGLTYREGKLVQALTRGDGTRGDDVTNNVKTIKSIPLAL
                     WGDFPFEFEIRGEIFMPRSSFDRLNAEREEIGQPLFANPRNAASGSLKMQDPAEVAKR
                     NLDCYLYFMMGDNLPAGNHYQCLQKAKEWGLKISPYIARCFTPDEIFEFIKTWDSGRR
                     ELPFDIDGIVIKVNDFSQQVILGYTAKSPRWAIAYKFKAERVSTPLLSIDYQVGRTGT
                     VTPVANLKPVFLAGTTVKRASLHNADIIKKLDLHEGDHVFVEKGGEIIPKVVGADPAQ
                     RAFFAPAVKFITHCPECNTPLVRQEGEAAWFCPNDTGCPPQIKGRLEHFISRKAMNID
                     SLGEGKVELLYDKNLVSNIADLYDLTFDQLLGLEKTYENEDGLIERKISFREKTVNNI
                     LAGIEQSKSTPFERVVYALGIRFVGETVAKKLARHFGDIDAIMQAGFEELKQVEEIGE
                     KIAGSVVEFFSKSENRAIVERLRQKGIRMQNDQSVVEIAENKLGGKSFVISGVFARHS
                     RDQLKEMIEKYGGRNTASVTGKTDYLLAGEGMGPAKLKKAGDLGVRIISEEEFLEMIT
                     TV"
     gene            complement(7874..8605)
                     /locus_tag="PKZ74_04415"
     CDS             complement(7874..8605)
                     /locus_tag="PKZ74_04415"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPM92033.1"
                     /translation="MKMQKFVTHFALLIILGLISTTSYGCRNFPEPRGQENPVKEERN
                     IANFTKLDIGGAFEVILSQGDQVKLVVEAEADELKDIKTEVNGSTLKIYTESTWNPRY
                     HEMRIYLTITKLESVDFSGAVEVRSAGTLSFTDLELNVSGATEIELNLTANKLDAEFS
                     GASEIDLTGKATSGRFELSGASDFDAENLEFQDLSVEVSGASDAKVWATGTLNIDASG
                     ASTIRYKGNPRISLDESGASSVKPL"
     gene            complement(8627..9688)
                     /locus_tag="PKZ74_04420"
     CDS             complement(8627..9688)
                     /locus_tag="PKZ74_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92034.1"
                     /translation="MKTLLFTYRKSLRTIVLLFSVLLLDPLSGTGQEYTKIQDGSYNV
                     VKGAELTIRNKFGKINCLAWDESKVTIHVIVKVNASSQEKADRVLNKINVELSGTQTK
                     VEGITTVGSISNSDFSIDYDIRMPRWMKLDLNNQFGDIYLDETEGDAKINLQYGELEA
                     RSFKGNSTEMSVKFSDAEIDYIKDGNINIEYSDWESENADNLRFYSRFSDLEAETSGK
                     LVLDSQYDDVTINSAGEIILITRFTDAEIKKINGKFDFDSQYGELNVEYIAPGFVEGK
                     VRNSFSDVNMTFDPKVNINVDAEMQFGELNYPKSASISHQTENYTTQVYKGKIGSGTT
                     AGQLTIIGKNANARISFSE"
     gene            complement(9699..10220)
                     /locus_tag="PKZ74_04425"
     CDS             complement(9699..10220)
                     /locus_tag="PKZ74_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92035.1"
                     /translation="MLEEKIRKNRDLFDHAEPEQGHYDRFGSKLDFLHHDTSQGRTRH
                     LSGFMKVAAVFIGLFALSVVFYLVTPYGQSNELAANDLPQEIQEARMYYDKLAQEKIE
                     KINECAASSSEASYIQKVVSEEIIMLDSSSVQLEEQLQSDKENKKLINALIRNYKTKS
                     DLLDDILNRLCHI"
     gene            complement(10239..10817)
                     /locus_tag="PKZ74_04430"
     CDS             complement(10239..10817)
                     /locus_tag="PKZ74_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92036.1"
                     /translation="MEQPVKNIHVDLIRGCRENDRKAQIQVYKLYYKSMFNTALRIVN
                     DPALAEDIMQETFLEAFRQIGTYREESSFGAWLKRITVNKAIDEFRKTKDVVSIDQMD
                     TEVADTDDDENYLQVLSTRVEEIRKAIHALPDNFRVILSLHLLEGYDHEEITQILGIS
                     YSLSRTRYSRAKRKLLELIGACENNMTNSKLN"
     gene            10953..11855
                     /locus_tag="PKZ74_04435"
     CDS             10953..11855
                     /locus_tag="PKZ74_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM92037.1"
                     /translation="MKKNILLHFGIYPIILLAMMFWGMSFVWSSIVFKYYNPITTIFL
                     RLVISTLLLSLFIYFTGKFERLKKEDRILFLLSSLFNPFLYFMGENFGLKNSSPTISA
                     VIIATIPLFAPVAAYLALNEKITWLTIAGIFISFIGVGIMLVNPDLTLNASPAGIAFL
                     LGAVLSAVIYSVFLKKLTLRYSSLTIITYQNALGILYFFPLFLIFDFNHFLTVKPNHE
                     LISSLLQLSVFASSLAFVFFTMAVKELGVSRTNVFSNLIPVFTAIFSAIFISEMFTAT
                     KIAGMAAVIAGVMISQWKRRTVGR"
     gene            11941..13080
                     /locus_tag="PKZ74_04440"
     CDS             11941..13080
                     /locus_tag="PKZ74_04440"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPM92038.1"
                     /translation="MTKISVLGAGLVGFPIALDLSKDAGFQVTLYDIRKERLQRITQK
                     HKLVTVQADLSDESMVREIARKSDLVVSAVPGHLGFQTLKYIIEEGKNVIDIAFFPED
                     LFELEELAKKNDVIAISDIGVAPGMSNILTAYCSKKLDYAEKARIYVGGLPVARQLPF
                     EYRAPFSPIDVIEEYTRPARFIRDGKLVEMPALTEPELMNFAGIGTLEAFNSDGLRSL
                     IRTIKCPDMIEKTLRYPGHLAKIQLLKDIGMLSTEPVDVKGQQIRPIDLTTQLLFPMW
                     DLKNEEDLTVMMVMVEGTKDGKKMRFTWELSDRFDADTGIHSMARTTGYTATAAVRMV
                     TSGIYTRKGVSPPEYIGFEPQCVEFMLHDLAMHNVIYTETTEILP"
     gene            13082..>13343
                     /locus_tag="PKZ74_04445"
     CDS             13082..>13343
                     /locus_tag="PKZ74_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM92039.1"
                     /translation="MPTQRDLFFRYLGLPSLNAPALEISRAAGVFLYGPDGKDYVDLV
                     SGVAVSNLGHQHPDIVNAVREQAGKHLHLMVYGDLVQSPQVLL"
BASE COUNT         3619 a         3147 c         3074 g         3503 t
ORIGIN      
        1 caacaggcgg cgcatggccg ggcccctgcc ctggccacag gtatcaagcg tacatggccg
       61 aacaaagtcg ttttcacata ccagggcgac ggcgacctgg ctgccatcgg taccgcagaa
      121 accattcacg cctgcaaccg gggtgaaaac attattatct tctttattaa caatggtata
      181 tatggtatga cgggtggcca gatggcgccc accacgctgc ccaatatgaa gtcgtccact
      241 tctccgtacg gaagggatac tcaaattatg ggacatccgc tgaaaattac cgaactggtg
      301 gctcaactcc cgggtactta ctttgttacc cggcaggctg tccatactcc ggctaatgtg
      361 agaaaaacca aaaaagccgt cagaatggca ttcgagaacc agttaggcaa taagccgggc
      421 ttatcattca ttgaaattgt atccaactgt aactcagggt ggaagatgac acccgtccag
      481 gctaaccagt ggatggtgga taatatgttc cctttctatc cgcttggcga tataaaggtg
      541 gatggagcgc tggtcgcgaa atagtcatta gtcattggtc gttagtcaaa ttaataaatt
      601 atgacagaag aaattatcat agcaggtttt ggcggacagg gtgtactgtc gatgggtaag
      661 atcatggcct attccggtat catgcaagac caggaggtta gctggatgcc ctcctacggg
      721 ccggaaatgc gcggcggaac agcaaacgta acggtgatac tcagcgatga aaggatcagc
      781 tcccctatcc tgacccggta cgacaccgcg attatcctga accaacaatc gatggacaag
      841 ttcgaaccga tggtgaagcc gggcggaacg ctgatttacg acgccaacgg catcacccgg
      901 catcccgaaa ggaaagatat aaagatttac cgtgttgatg cagccgatga agcctcgaaa
      961 atgggtttgt ccaaaatctt caacatgatc gtcctgggag ggtttctccg gatcaggccg
     1021 atcatttcgc tggaaaatgt gatccaggga ttgaagaaat cactcccggc ccggtatcac
     1081 catttgatcc cggataatga aaaagccctt aaaagaggca tggagatcat cgcgcaggtg
     1141 aactgaaacc ctgtcagact caatcacaat tttttatact ttagcgcttg cataatactg
     1201 tttgcatggc gccaattcat aacaggaacc tgaaaatacc ccattttcac agcctcaggg
     1261 tatatttggt ttccacaatc ctctactttt ttctggtcct gccggtggcc agtattcttg
     1321 cattgaaata ttatataccc gactatcttt accagaaaag tgagcctgct gcaatcgcaa
     1381 atcctgatat ttctacagac cgtcaggtgt atgatccgac acctgatacc cttggtaaaa
     1441 ccgtagattc cattcgccgg gcggagaata attctctggt caccgtcagg ttctccaccc
     1501 ccgattcacc gcagtttcaa ataactaccg gtgagaacac cagcgaaaac agccgtttcg
     1561 gtggcacaat ctccgttctg atgaagctgc tttttgtgag ctttatactg ggtctttttt
     1621 tcaaccttcc tttcaaaatc tatttcagca aaaaaagaaa tctcaaaccc attaaacccg
     1681 gcgtgtttca atattgccgc cggctgattt tgaaagtccc cctcatcaat acgggtatcc
     1741 ttgcccttcc ctacctgatc acactgctgt atatgcttta ttttattttg ttcagaagag
     1801 atgaagatcc gctggaccgg caattttaca cgcagtactt tttcatcaca ctggttgcag
     1861 gaatactgac tctgatgctt gtgttcttct ggctcaaaca ccgtgttcat atccgatatc
     1921 ttgaacatat atattctccg gatgaattaa agaaaaggat ctttaaaggc aagccgggga
     1981 ggattaaaaa ccggctctgg atttccagcg ccatgaccac cctgtttccg ctcattatcg
     2041 ttgtatttta tattgtcctg agcatctcct cggttgaaga acttagcctg ctaaaccttt
     2101 cggacgaaaa ccagaaaatc ctgctgggca aatacatagc gtttgacctg ggacttgacc
     2161 tgcccacgct caatgaattc ttctatgtta acgccgtgaa cagcttcctg atggttgtcg
     2221 gtatcgggac cggcgtattc atcgcgctga tctatgtcgt tctgtttgtc agatggacaa
     2281 cggaggatat aatctacccc gttaacgaac tgctggccaa tatgcagcgg acaggccgcg
     2341 gcgaactgga tatctacagc attgtcagaa cgaatgacga agttggcgcc ctgacggaag
     2401 gctacaatga gatgaccgga aaattaaaaa gttattttga aagccttgac aaaatcaacc
     2461 ggtcgaatgc ccgctttgta ccccggcagt ttctcgattt tctcggcaag gaaaatatta
     2521 ccgaaatcca actgggcgac caggttcagc aggagatgac catcatgttc tccgacatcc
     2581 gtgatttcac ctctatctcg gaatcgatga ccccaaagga gaactttgat tttatcaaca
     2641 actacctggg atatatggaa ccggtcatcc ggacaaataa cggtttcatc gacaagttca
     2701 tgggcgactc cattatggcg ctctttcccg aaagaagtga agacgccctg aacgcctcga
     2761 tcgaaatgcg gattaagctc ctggaattca atgagatcat gggtcagttc gggaaacctg
     2821 ccatcgatgc agggattgga atacataccg gcttgctcat gctcggtgtg atcggtggtg
     2881 aaggacggat ggacagcacc gtaatatcgg atgcggtgaa cctggcttcg aggctggaag
     2941 ggctgaccaa aatttacggc ggatcgatta tcattacgga agatacgctc atcaaactga
     3001 acgatccgaa tcattaccat ttcaggttcc tggatgtcgt aaaagtaaaa gggaagaaag
     3061 aagccatata catcttcgag atcatggacg gtgaaccgga aaggatcaga aacctgaagc
     3121 tcatgaccaa agacgattac aataaggcgc tgcaatccta caagaaaaag gaatttgaga
     3181 aagcgctcaa cctgcttcaa aacatagaag cggtaaatcc tgacgataaa gccgttaaaa
     3241 tatatatcga ccgttgtaaa cattatttaa aaaatggtgt tcctgacatg tgggatggag
     3301 ttgcaagttt tgaaatgaaa tattgataaa cattttccgg gctggttatc cggttaaaca
     3361 gaaaggtttt aagatgaatt acgaagccgc caaaaaatat atcatcacga gattagaaaa
     3421 agaactggac ccccggctca cctaccattc cctttcccat acgctagatg ttctcgaatc
     3481 agccgtcaga ctggcagata tggaacaatt gccggagacg gaaaagctct tgctgatgac
     3541 tgcctgcctt tatcacgatt cggggatgtt gataacatat cgcggacatg aagacgccag
     3601 tatcatgatc tgccgggaga aactgcctga gttcgaatat tcagcggaag aaatctcagc
     3661 cgtatgcagg atgatcaata cgaccaaact gccgcaatgc gccaataaac ccgccgataa
     3721 aattctgtgc gatgctgatc tggattattt aggccggccc gattttttta tgatctccca
     3781 caagttaaaa tatgaatggg atattttgaa cattcatccg actagtttgt ttgaatggta
     3841 ccagatccag aaagattttc tgtcaggcca cagatatttt acaaaatcag cgaaagagtt
     3901 gcgccaggaa ttcaaaatgc agatcctgag tcaaatcgaa tttatgtgca atcatcataa
     3961 ataaacataa tcgtgctgag tggataattt tctatttttg ctttaatcaa aaaaaaatca
     4021 actgttcatg gaaaccgcaa aaaatcccat ttttcttgta ccaaccgatt tttcggaagt
     4081 gtgcgccaat gctgcccacc gtgcagcttc tcttgccaaa gacttcaatt ataaagtcgt
     4141 cctgctgcat gtgattgaca ggaacaccat cagcgaactg aagaaagagg gtaaaggtcc
     4201 tgaatgggtg gatgaaaagc tgaaagacct gtcgaacgcg ttaaatcttg aatatggtgt
     4261 taaggttgag accatttcac aggaaggcga tatatttaca acgattgcag aagtggccaa
     4321 cgacatcaaa gccagcctga tattcctggg aacccacgga aaagtgggaa tgcagaaaat
     4381 tacgggcagt tttgcgctga aagtcgttac cagctccgaa ttacccacca ttgttgttca
     4441 gaaaaggccg tttggtaaag ggatcagcac tatcgttatg ccgatcactt ctgacgcagg
     4501 tccctgggcc aaaacaaaat gggcggcatc cattgccaaa gaattcaact cgactatcca
     4561 tattttccac cttccggggg aagagatgga agacgtcatt accatgataa ccaatcattt
     4621 ccaggttaac ggggtgaaat ttgaaataaa agaagcggaa aaagcaacca gtttcaccaa
     4681 acaggtcatt gattattcca ctgcaagaaa tgcggatatg atcctgatca tgacaaatcc
     4741 tgataagagc ctgaagtcgt tcctcctggg tagctacgac gaagatatca tcttcaatac
     4801 atcacagatt cccgtcatgt gcatcaatcc gcgtgatttc aactggaaaa aaattatccc
     4861 ccggtaaata cgggcccttt cttacacttt taaccaaata actccggctt atcatgaata
     4921 taacatcgga caaggagaat attatcctgg ttcccaccga cttcacaccg gtgggccaga
     4981 atgccataga tcacggggta aaggcttgtg aaatactcgg attcaaactg gttatccttc
     5041 acgttatcaa tagagaaacc cgggcggtct ttggcaagac cgatacagaa aaagctgttc
     5101 aacagagact tgctgagatt gcggaagcag ttaagacgaa atcgaccatt gaggttgagg
     5161 tggccgcacg cgaaggaagt atattcgatg tgatcagcga agtcgccaaa gagttaaaag
     5221 tcaacctgat gatcctgggc acccatggca aaagaggcct tcagcatcta atgggaagct
     5281 atgctatgaa ggtcattacc cagtcgcctg ttccggtcat tgtagtccag aaaaggcaga
     5341 tcggccctgg cggttacagg aaagtcgttt tcccgataag cctccataca gaatcacgtc
     5401 agcaggtgtt ttacgccatt gtcactcaat tgctgtttaa aggcgagatc cacatcttta
     5461 aacagaccgg caataacccc gaggataata ccaaactgcg gatcatcaca tcacaaatta
     5521 ccgaagaatt tgacaataag cacgttagat acagcctgac cgaagccgac agggcatcaa
     5581 acttcaccgg tcagttgctc gattttgccg tatcggaaag tgccgacctg atcatgatga
     5641 tcaccgacag cgacagcatc caccctactt tcgataatag ttcgtggagt gaaaaactga
     5701 tctttaatat cgcccaaatc cctgtatttt gcatcaaccc ggtatttctg gggcagactt
     5761 attattcatt ctgaaaagac aatatcctga catccgaaaa aatccgacct ttgtataaag
     5821 ggtcttgatg ttatgacaac agcagaagcc agaagccgga tggaatggct ggccggagag
     5881 atcaaccggc ataactaccg gtattacgtt ctctctgatc cgttgatttc tgattacgaa
     5941 ttcgatatgt tactggagga actggttcat ctggaaaacc tgtatcctga actggcaaca
     6001 gccgactccc cgacaaaacg ggtcgggacc gatctgacga aggaatttat gcaggtggat
     6061 cacaggtacc cgatgctctc actcggtaac acatattcag agcaggaaat cattgagttc
     6121 gacggaaggg tcaggaaatt gctggataat gaagcgcccg aatatgtctg cgaactgaag
     6181 tacgacggcg ttgccatagg acttacatac cgggaaggga aactggtgca ggcgctgacc
     6241 cggggcgacg ggacccgggg cgatgatgtg acgaacaatg ttaaaacgat taaaagcatc
     6301 cccctggctc tctggggcga cttccccttc gaatttgaaa taagaggcga aatcttcatg
     6361 ccccggagca gcttcgacag attgaatgca gagcgtgaag aaattggcca gccgttgttt
     6421 gccaatcccc ggaatgcggc atccggaagc cttaaaatgc aggatccggc cgaggtggcc
     6481 aaaagaaatc tcgactgcta cctttatttc atgatggggg ataatctccc ggccggcaat
     6541 cattaccagt gccttcaaaa agcgaaggaa tgggggctga aaatatcgcc ctacatagcc
     6601 cggtgtttca cgcctgacga aatctttgaa tttatcaaaa cctgggattc aggccgcagg
     6661 gaactcccct tcgatatcga tggaattgtg attaaagtca acgatttctc tcaacaagtg
     6721 atcctgggct acactgccaa atcgccccgc tgggcaattg cctataagtt caaagccgaa
     6781 agggtttcga cgcccctgtt gtcgatcgat taccaggtag gacgcacggg caccgtgaca
     6841 ccggtggcta acctgaaacc ggtgttcctg gccgggacta ccgtaaaacg ggcatcgctc
     6901 cacaatgccg acatcattaa gaaacttgac ctccatgagg gtgatcatgt ttttgtggaa
     6961 aagggcggcg agatcatccc gaaagtagtg ggcgccgatc cggctcaaag ggcttttttt
     7021 gcaccggctg tgaaatttat cactcactgc ccggaatgca acacaccttt ggtaaggcag
     7081 gaaggtgaag cggcctggtt ctgccccaac gatacgggtt gcccgccaca gatcaaaggg
     7141 cgcctggagc atttcatcag ccgcaaggca atgaatatcg acagcctggg agaaggaaaa
     7201 gttgaattgc tctatgataa aaacctggtc agcaatattg ccgatttgta cgatctcacc
     7261 ttcgaccaac tgctgggact ggaaaaaacg tatgaaaatg aagatggtct gattgaaagg
     7321 aagatcagtt tccgggaaaa gacggtgaac aatatcctcg cgggtataga acaatcgaaa
     7381 tccacaccct tcgaaagagt cgtctatgct ttgggcatcc ggttcgtcgg cgaaacggtg
     7441 gctaaaaaac ttgcccggca tttcggcgat atcgatgcca tcatgcaggc cggtttcgaa
     7501 gaattgaaac aagtggaaga aatcggagaa aagattgccg gttcggttgt tgaatttttt
     7561 tccaaatcgg agaaccgcgc tattgttgaa agattgaggc agaaagggat ccggatgcag
     7621 aacgaccaaa gcgttgtaga aatcgcggaa aacaagctcg gcggaaagtc atttgtgatc
     7681 agcggcgttt ttgcccgtca ttcgcgcgat cagctcaagg aaatgattga aaaatacgga
     7741 ggccgcaata cagcctccgt aacggggaaa acagattatt tgctggcagg tgaaggcatg
     7801 gggccggcga agcttaaaaa agccggcgac ctgggcgtaa ggatcatctc tgaagaggaa
     7861 ttcctggaga tgatcacaac ggtttaacag aggaagcccc cgattcgtcg agagaaatcc
     7921 tgggatttcc tttgtaacgt atcgtagatg cgccggaagc atcaatattt aatgttcctg
     7981 tagcccacac tttggcgtcc gatgcaccgc ttacttctac cgaaagatcc tggaactcaa
     8041 gattctctgc atcaaaatcg gaagctcccg acagctcaaa ccttccggaa gtggctttac
     8101 cggtcagatc gatctcggag gcgccactga attcggcatc caacttattg gctgtcaggt
     8161 ttagttcgat ctccgtggcg ccgctcacat tcagctcaag gtccgtaaaa ctcagtgtgc
     8221 ccgcgcttct cacttctacc gccccactga agtcaacaga ttcgagcttc gtaatcgtca
     8281 ggtaaatcct catttcatga taacgtggat tccaggttga ttcggtatag atcttcagcg
     8341 tgctgccatt cacctcagtc ttgatatctt tcagttcatc cgcctcggct tctacaacaa
     8401 gctttacctg gtcaccttga gatagaatga cttcaaaagc tccgccgata tccagtttcg
     8461 taaagttcgc gatgttgcgt tcttctttaa caggattttc ctgaccccgt ggttccggaa
     8521 aattccggca tccgtatgat gtagttgata tcaatccgag gataatcaat aacgcgaaat
     8581 gggttacaaa tttttgcatt ttcataattt attgaattaa aatgatttat tccgaaaaac
     8641 ttattcttgc gttcgcattt ttccctatta tggtgagctg gccggccgtt gttccgctgc
     8701 ctatcttacc tttataaacc tgtgttgtat aattctcggt ctgatggctg atggaagccg
     8761 attttggata attcagctca ccgaactgca tctcggcatc tacattgata tttacctttg
     8821 gatcaaaggt catattgacg tcagagaatg agttcctgac tttgccttct acaaatccgg
     8881 gagcgatata ttcaacattt aattcgccat actggctgtc gaaatcgaac ttaccattaa
     8941 tctttttgat ttccgcatcg gtaaatcgtg ttatcaggat gatctcgccc gcgctattta
     9001 tcgtaacatc atcatattga gagtcgagca ccagcttacc gctggtttcc gcctctaaat
     9061 cgctgaaacg tgagtaaaac cgcaggttat ctgcgttttc agattcccag tcgctgtatt
     9121 cgatattaat atttccatcc ttaatataat caatctccgc atcagagaat ttcaccgaca
     9181 tttccgtcga atttccttta aacgagcggg cttccaattc gccgtactga aggttgatct
     9241 ttgcatcgcc ttcggtctca tccaggtaaa tatcaccgaa ctggttgttc agatcgagct
     9301 tcatccaacg gggcatccga atgtcgtaat caatactgaa atcgctgttg ctgatgcttc
     9361 cgacggtagt gattccttct acttttgtct gggtcccgga aagttctaca tttatcttgt
     9421 taagcaccct gtcggctttt tcctggctgg aggcgttcac tttaacaatc acgtggatgg
     9481 tgactttact ttcgtcccag gccaggcagt tgatctttcc aaatttattc ctgatggtaa
     9541 gttcagcgcc tttcacaaca ttgtaactgc cgtcttgaat tttcgtgtat tcctgccctg
     9601 ttcctgataa aggatccagc agcaatactg agaagagcaa cacaattgtc ctgagggatt
     9661 ttctgtaggt aaaaagtagt gttttcatct ggtttttatt agatatggca taacctgttt
     9721 aaaatatcat cgagcagatc cgattttgtc ttataatttc tgatcagtgc attgataagc
     9781 tttttgtttt ctttgtcgga ttggagctgt tcttcgagct gcaccgaact ggaatccagc
     9841 attatgattt cttcacttac aactttctga atgtaagagg cttcggaaga tgatgcggca
     9901 cactcattga ttttctctat cttttcctgt gccagcttgt cgtagtacat tcgggcctcc
     9961 tgtatttcct gaggcagatc attagcagct aattcattcg actggccata aggggtgacc
    10021 agatagaaaa ccaccgataa agcaaacaaa ccgatgaaga cggccgccac cttcataaat
    10081 ccgcttaaat ggcgggtacg gccctgtgag gtgtcgtggt gaaggaaatc cagtttcgac
    10141 ccgaaccggt cgtagtgccc ttgttccggt tcggcatgat cgaaaagatc cctgtttttt
    10201 ctgattttct cttccaacat tgacatggtt atatcctgtt agttcagttt tgaattcgtc
    10261 atattatttt cgcatgcccc gatcaattcc agcaatttcc ttttcgcccg tgaatacctt
    10321 gtccgggaaa ggctgtagga aatgccaagg atctgggtta tctcttcatg atcatagcct
    10381 tccagcagat gaagggataa gatgacccgg aaattgtcag gaagggcatg gatcgctttc
    10441 cgaatctctt ccacccgggt tgaaagcacc tgcaggtaat tctcatcgtc gtcggtatca
    10501 gccacttcag tatccatctg atcgatcgaa accacatctt tcgtcttcct gaattcatct
    10561 atcgctttat tcactgtaat ccttttcagc caggcgccaa agcttgattc ctcccggtat
    10621 gtgcctatct gacggaaggc ttccaggaaa gtctcctgca tgatgtcttc ggctaatgcc
    10681 gggtcattga caatccttaa tgctgtgttg aacatcgatt tgtaatagag cttatagacc
    10741 tggatctgcg ctttacggtc attctcccgg catccccgga tcagatccac atgtatattt
    10801 ttaaccggct gttccaaaat caggaatttt taatctaagg tcggatgaaa aaaaggattg
    10861 ttacaatttc aggtagattt tttaagggga tcatggaaaa taaggttaag taaactttat
    10921 acctttgggc gcttcatttt tttgtcccgg cgatgaaaaa gaatattttg ctgcatttcg
    10981 gcatctaccc tatcatcctc ctggccatga tgttctgggg aatgtcgttt gtctggagtt
    11041 caattgtttt taaatattat aatccaatta ccacgatctt tctccggctg gtcatatcta
    11101 ccttattact atcactgttc atctatttta cgggtaagtt tgaaagactt aaaaaagagg
    11161 acaggatact gttcctgctt tcctcgctgt tcaatccctt cctgtatttt atgggtgaaa
    11221 acttcgggct gaagaattct tccccaacca tcagcgcggt tatcatagct accattccac
    11281 tgttcgcccc ggtagcggct taccttgcat tgaacgaaaa aataacctgg ttgaccattg
    11341 ccggtatctt catctcattt atcggtgtcg ggatcatgct tgtaaaccct gacctgacgc
    11401 tcaatgcttc ccctgcagga atagcgttcc tgctcggcgc cgtcctttcg gccgttattt
    11461 attctgtttt tctgaaaaaa ctcaccctgc gctattcatc cctgaccatc atcacctacc
    11521 agaatgcact cggaatactc tatttcttcc ctttgttcct gatctttgat ttcaatcatt
    11581 ttctcacggt aaaaccaaac cacgaactga tttcatcatt gttgcagtta tcggtcttcg
    11641 catcgtctct tgcctttgtg ttctttacta tggccgtgaa ggaactgggt gtgagccgca
    11701 cgaatgtctt ttcaaacctg ataccggtgt tcacagcgat tttttccgct atctttatct
    11761 ccgaaatgtt caccgcaacg aagattgcag gcatggccgc agtgattgca ggagtgatga
    11821 tttcacagtg gaaaaggagg acggtaggaa ggtaggacgg taggacggta agattagaat
    11881 ggccaatgac gcgagcacag cgagcttatg acaactttat aactacttta taactacctt
    11941 atgacaaaaa tctcagttct cggcgcaggc cttgttgggt ttccaatcgc tcttgactta
    12001 tcgaaagatg caggattcca ggtaacccta tacgatatcc ggaaagaaag gctgcaacgg
    12061 attacccaaa aacacaagct tgtcactgtg caggcagatc tttcagatga atccatggtc
    12121 agggagattg cccggaaatc ggaccttgta gtcagtgcgg ttccggggca tcttggtttc
    12181 cagaccttaa aatatattat cgaagaagga aagaatgtca tcgatattgc attctttccc
    12241 gaagaccttt tcgaactgga agagctggca aaaaagaatg atgtgattgc catttccgat
    12301 atcggcgtgg cgccggggat gagcaatatt ttgacagcct actgctccaa aaaacttgac
    12361 tatgccgaaa aagccaggat ttatgtcggc ggcctgccgg tggccaggca gcttccgttt
    12421 gagtaccggg ctcctttctc cccgatagat gtcattgaag agtacacccg cccggcgcgc
    12481 ttcatccggg atggcaaact ggtggagatg cctgccctta ccgagcccga gctgatgaac
    12541 tttgccggta tcgggaccct ggaggccttc aacagcgacg gcctgcggtc gcttatccgt
    12601 acgatcaaat gcccggatat gattgaaaag accctgcgat accccggcca tcttgccaaa
    12661 attcaattgc taaaggatat agggatgcta tcaaccgaac ctgttgatgt caaaggccag
    12721 caaatcaggc cgatcgacct tacaacgcaa ttattgttcc cgatgtggga tttgaagaac
    12781 gaggaagatc tgactgtaat gatggtcatg gtggaaggta caaaggacgg gaagaagatg
    12841 agattcacct gggaattatc cgaccggttc gatgcggata caggcatcca ttccatggcc
    12901 cgcaccacgg gatataccgc cacagccgcc gtcagaatgg ttacttcggg gatatacaca
    12961 cgaaaagggg tatctcctcc cgaatatata ggatttgagc ctcagtgcgt cgaatttatg
    13021 ctgcacgatc tggccatgca caatgtgatc tacacggaaa cgacagaaat tttgccataa
    13081 gatgccgaca caacgcgatc tgttcttccg ctacctcgga ttaccgtcgc tgaatgctcc
    13141 ggcgctggag atcagccgtg ccgccggcgt tttcctgtat ggccctgacg gtaaggacta
    13201 cgtcgacctg gtttctggcg tagctgtgag caacctgggg caccagcacc ccgatattgt
    13261 caatgctgta agggaacagg ccggaaaaca tttacatctc atggtatatg gcgacctggt
    13321 acaatcgcct caggtgctgc tgg
//
LOCUS       DAOPNH010000055        13296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4756_length_13296_cov_6.175893, whole genome shotgun sequence.
ACCESSION   DAOPNH010000055 DAOPNH010000000
VERSION     DAOPNH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4756_length_13296_cov_6.175893"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2567
                     /locus_tag="PKZ74_04450"
     CDS             <1..2567
                     /locus_tag="PKZ74_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM92040.1"
                     /translation="KVIAYEKNPDMSGNWYGAAIGIASSEGAGIGDDGESDQAHETVI
                     YNDKLDPFTYGTFTQVYDPGATAGMVTNAVNTGASVINYTGHGWGDGWGTTGFSSSNV
                     AGLTNGDKLPFIVSVACNNGDFDLGTCFAESWLRKSGGGAIMFLGASISQPWAEPMRG
                     QDYFMDILIGGYDYSAHPGQNGISTTEQRTTLGSAVFNGLTLMCVESGGGSDWETVKT
                     WNYFGDPSVQARTAMPLQLTVSNTVVMVGIPFTTMVTTSQGPVANAMVTLSQDGLYFT
                     GVTDDDGNVAIEQALNPGTAKLVITAFNTGTIYQDVTVTPPDGPFVTISSVEINDIQG
                     NNNQLLDYGETVYLTISLTNLGTVGAEDVSAVIATTDEFITLTDVEEDYGTIPAGEVV
                     TITSGYELSALESLPDMHIALFNLEITGTAARETWSSSFVLPGHAPVLGMAGYTIDDA
                     SGNGNGRLDPGETALLQIGAVNDGSSGAFNVEGTLTCDNEFVTINGATVTYGDIDAGA
                     SVMQSFEVVISPDADAGTAAQFIFDLAGDMNITGQDDIIEYVGQIPVLLLDWDGNNNS
                     SDDMELCFANLEVGYDKMDVIPEERNLYTSIFVCLGTYDNNHVLSAEEGELLAEYLDN
                     GGNLYMEGADTWYYDPQFTPTPLHAMFNILGVEDGADDLEQLIGQPGSIVEDMFFGYN
                     GENSYMDHIDAIPPAQVMFMNEAPSYGTMVSYDGGTYRTVGSSFEFGGLVDGDMTKDD
                     LMIRILDFFGVDGIWTGQDERAAGDISTGSYPNPFDQTTVISFKLTEPGSVMVDIFDI
                     SGRNIINLFEGQATTGVTEIQWNGRDHTGKDVAPGIYFYRVDAGLSRSTGKMMKIR"
     gene            complement(2569..3288)
                     /locus_tag="PKZ74_04455"
     CDS             complement(2569..3288)
                     /locus_tag="PKZ74_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM92041.1"
                     /translation="MAKHWVIPDIHGNRKTLQALIEDQIKPTRYDTLYFLGDYIDRGN
                     DSKGVIDYIIRLQEDDYTIRTLRGNHEDYLIRTFDNETVRKNLLGISFRNQLKKEWYK
                     YGGKETLKSFGVSDVHEIPEKYIEWMRGLEFFIELNSYILVHAGLNFGIDNPFDDRHS
                     MLWIKEFTVDMEKTGNRKIIHGHVPVSLDFIGVLRTSSTFKFIDLDNGVYMTGKEGFG
                     NLVALELDSLEWKIQPNADIL"
     gene            complement(3295..4041)
                     /gene="gpmA"
                     /locus_tag="PKZ74_04460"
     CDS             complement(3295..4041)
                     /gene="gpmA"
                     /locus_tag="PKZ74_04460"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPM92042.1"
                     /translation="MNRLVLLRHGESAWNKENRFTGWVDVGLSEQGIREAREAGRVMK
                     EKGFRFKTAYTSYLTRAIKTLWLALEEMDLMWIPEKKTWRLNEKHYGMLQGLNKIDMA
                     EKYGSEQIQLWRRSFDVLPPPIPEDDEKNAKFDPRYAALKDEDIPRTESLKEVIARIM
                     PLWQEDISKTLLAEKEVLIAAHGNSLRAIVKYLKKMSNEEILEFNIPTGIPYVFEFDD
                     DLNLLNDYFVGDPEVIRQLMEKVANQTKKK"
     gene            4173..7361
                     /locus_tag="PKZ74_04465"
     CDS             4173..7361
                     /locus_tag="PKZ74_04465"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM92043.1"
                     /translation="MDLIYKKLNRMIGFILFGVASLVYIITSEPTTSFWDCGEYIATA
                     FKLQVGHPPGAPIFQLLGRFFSLFAFGDTSQVARMINTMSALSSGLTIAFLFWTITML
                     AKKLLAKMEMTRANLIAIFGSGVVGALAFTFSDSFWFSAVEGEVYAMSTLFTAMTFWA
                     ILKWEAVAGEPYSYRWLILIAYLIGVSIGVHLLNLLAIPAITFVFFYKNYNIPGWKGF
                     FLALGISFLLVALILYGIVPEIVSLFANTELFFVNTLGLPFNSGTYFFILLLVALIVF
                     GLLYTHSDNKLYPRIIIGLGAVTVVLFMLETSSAGNFFGRLIAASAVVGLFYMWRNRK
                     ALLNNIIIAFVFILVGYASFFTLVIRSNANPPIDENNPENAISLLAYLNREQYGSFPI
                     FHGQYYNAPLIDREDGKPVYKRDDASGKYIVWDSREGTVPVYDPEYTTIFPRMWNNQE
                     DRYIQDYKNWAGIRNDPDNKHIPTFSENLRYFFRYQVNHMYIRYFFWNFVGRQNDNQG
                     MYADLLNGNWISGIGFIDKMRLGPQDVPGSMSNEARNTFFLLPLLAGILGLLYQFGKN
                     AKDGTVVLLLFIMTGLAITIYLNQHSPQPRERDYAYAASFYAFTIWIGLGVLWLYELL
                     QKKLSHKIAAITASAAMFLLVPVIMMAQGWGDHDRSGRYTALEVAKNYLNSCEKNAIL
                     FTNGDNDTFPLWYAQEVEGIRTDVRVCNLSLLNTDWYIDQMLRKAYDSDPLPFTLPET
                     MYKNGSHDVTYLLEEQNPNKEFIEVKAIFDRLKKNEKIFQVNTGRGMADYFPTKTFRI
                     TVDPAVAVSSGTVPAELAGQVTDLEWRISANALTKNYLMMLDLLAHNNWQRPVYYVST
                     TSRDAYIGLDNYLRLEGFAYRLVPIRQTVDKNETGGVNTDAMYDNLMNRFEFNINKPG
                     LLVSEDIYRMTVTMRSTYSRLIDALIAENKPARAVEVCDRIQTLIPGKKVPYNYFNLS
                     VADGYLKAGEADKGREILQELLRIQEDQLGYFFSFGDKHLPLLEMDIQQGVAVLSAVA
                     QVAESNGQDDLAEEADASLNRFYELYLGRVGN"
     gene            7374..8027
                     /locus_tag="PKZ74_04470"
     CDS             7374..8027
                     /locus_tag="PKZ74_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM92044.1"
                     /translation="MKDQLLTGIFTYVLPSALLWQVKTAEKAVYLTFDDGPIPELTDE
                     ILGILKQFDAKATFFCVGENVKRYPEIFARILQAGHSVGNHTHHHLKGWTTNYNDYIA
                     DISEAAGYINSGLFRPPYGLMTYRQAKILAKDYQVVMWSVLTKDYDPSVSKEGCLETA
                     IQGMRPGAIIVFHDNVKAKEKVLYALPRLLERMKEEGFVSERLVNGDGDPSSMVSSG"
     gene            complement(8170..8331)
                     /locus_tag="PKZ74_04475"
     CDS             complement(8170..8331)
                     /locus_tag="PKZ74_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92045.1"
                     /translation="MSTENLDCVKLMRKIRDKHHTEYEKNPSLREKRLAAIRKKYGIK
                     RKEKLQASH"
     gene            complement(8321..8794)
                     /locus_tag="PKZ74_04480"
     CDS             complement(8321..8794)
                     /locus_tag="PKZ74_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92046.1"
                     /translation="MAYRVYIDTSVIGGCFDEEFKTWSNLLVKELLSGQKVAIISDIT
                     IDEIQEAPKSVQEKLDEIMKSEFKELIAADQETRELAEFYISEGTVSEKFFEDALHIA
                     LATINKATVLASWNFKHIVNLERIRQYNAVNLKNGYSLLEIRSPREIVKFEENEY"
     gene            complement(9003..11171)
                     /locus_tag="PKZ74_04485"
     CDS             complement(9003..11171)
                     /locus_tag="PKZ74_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM92047.1"
                     /translation="MKNISLLLISVLFVLNFNAQNWEFFTPENTGLPSDHVFSVATDS
                     MGTMWFGTRSGLSRFDGTTWTNYNILNSGLPSDMIYAITIDKFGNKWLGTTGGIVKYN
                     GADWIVYNKSNSGLPCDTISTIAIDHLNNKWFGITPKYEMNQGYIRGGILCFNDTTWT
                     LYNSENSLLPSNYITALAIEESGAKWIGTYAGLAKLDGSEWTLLNAENSDLPSDSISS
                     ISIDKYNHKWIGFSFTNQESGHCFGGDGIAKFNDLTWQFYTKENSGLPSNNIYSIFID
                     EFENKWIGTTAGLVLYDDETWAVFNSSNTAMPDDRVNAVCIDTNGNNWISILTDNWAG
                     YYLGAGIAQFDGSGWTLYDHANTGLPYYWISSVAIDHSGNKWVGTYKNGLSSFDGSKW
                     TNYYRSNSGLPSDTINKIVIDSLGNIWIGTFGGVAVFDGNNWTVYNTSNSGLPSNIVY
                     SIFIDRLGRKWFGTKDGLALFDDNQWTISDIFWVSSICMDYSGNLWVGTYGNGLYKLA
                     DQVLTNFTVFNSDLPSNWVIDLTADNSGKLWMGADEFGLATFDGEKWVNYRKSNSGIP
                     DDQVISILLNDDGSKWIGTVNGFARFDDKTWEIFVPSLSGLPYNSFYSIARDSSGCLW
                     LGTFGGLAKFYNYPPLNISEADVSSLLIYPNPSISNLNIGNLSTGSTAEIIDISGRLV
                     FRKYMDSNSSLIDISDLQRGIYILKIQMQNRTLIERFVKQ"
     gene            complement(11183..12100)
                     /locus_tag="PKZ74_04490"
     CDS             complement(11183..12100)
                     /locus_tag="PKZ74_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM92048.1"
                     /translation="MNEIEFSVVVPVFNSEASLEELFHRLTEVLKATGKPYEMIFVDD
                     GSGDRSWEMLKKIKAENPDGFTAIRLAKNFGQHSATFCGFSFAKGKHIITLDDDLQCP
                     PEEIPKLIRTMEETDAELVYGIYRKKRHSAARNISSRSFKSLSRLFGSQTEGSSFRLI
                     SGSIIQKILGHHQNFVFIDEILHWYTDFIGYATVEHHPRKYKRSGYSRGKIWRFFTEV
                     MYHYSTAPLKVMVYGGMVSSVIFFIFSIYFILRKILMNVPLGYTSLIVAILFSTSLIL
                     LSLGVIGEYLSRIYMVQNKKPLYSVKKVL"
     gene            12334..>13296
                     /locus_tag="PKZ74_04495"
     CDS             12334..>13296
                     /locus_tag="PKZ74_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92049.1"
                     /translation="MKTTKILMCIFVMLVFIGLNTTKGQWLYNGTSIYNSNSGNVGIG
                     NNSPGSLLYVAKNMTEPTITVRNLGGTGGAAYTMTDDASGANWKFKATNNGGFKIRDH
                     ANLLDVITIEPNSTANAIYINAEGNLGVRTSTPAANFHVAENNPSYTAAFGNMISGWS
                     DGTNVSIGNDDFNSILYVGQDMSHKGYLIWNHHPEPDAATFMIGTYDGTNPMILQPAG
                     GNVGIGTSTPYARLDVMNNLYDYSRIGNTATVANEFLNYEETATVDGQCAIMTVRHRA
                     EQNDGAAYNIGQTNASIIGYNVWGDLFTFGSIGYNYNDFTRCGGA"
BASE COUNT         3406 a         3291 c         3091 g         3508 t
ORIGIN      
        1 ataaagtgat cgcctacgaa aagaatcccg atatgtcggg taactggtat ggcgctgcca
       61 tcggcattgc ttcttccgaa ggcgccggca ttggcgacga cggggaatcg gaccaggccc
      121 acgaaaccgt catttacaac gataagctcg atccgttcac ttacggtacg ttcactcagg
      181 tctacgatcc cggcgcaacg gccggaatgg ttaccaatgc cgtcaacacc ggcgcaagcg
      241 tgatcaatta caccggacat ggatggggcg acggatgggg gacaaccgga ttcagctcct
      301 cgaatgttgc gggactgacc aatggcgaca aacttccctt catcgtatcg gtggcatgca
      361 acaacggcga ctttgacctg ggaacctgtt ttgccgaatc gtggctgaga aaatcgggcg
      421 gcggcgccat catgttcctc ggtgcgtcga tcagccagcc ctgggctgaa ccgatgcgcg
      481 gccaggatta ctttatggac atcctcatcg gcgggtatga ctattccgct caccccggac
      541 agaacgggat cagcaccacc gagcaaagga caaccttagg ctctgccgtg ttcaatggcc
      601 ttaccctgat gtgtgttgaa tcgggtggcg gttccgactg ggaaacggtc aaaacctgga
      661 actatttcgg cgatccatcg gtccaggccc gtaccgctat gccgctccag ctcaccgttt
      721 ccaatacggt tgtcatggtc ggtattcctt ttaccacgat ggttacgact tcgcagggcc
      781 ccgttgccaa tgccatggtc accctcagcc aggatggact ttattttacg ggtgtgaccg
      841 atgatgatgg caatgtagct atcgaacaag ccctcaaccc cggaacggca aaactggtca
      901 ttacggcatt caataccggg actatttatc aggatgtgac cgttactcct cccgacggcc
      961 cctttgtcac tatctcatct gtagaaatca acgatatcca gggaaataac aatcagctac
     1021 tggattatgg cgaaacagtt tacctgacta tcagtctcac caatctgggt actgtcggtg
     1081 cagaagatgt atccgccgtt atcgcaacca ccgatgaatt catcacttta actgatgttg
     1141 aagaggatta tggcactatt cctgccgggg aagtggtgac cattaccagc ggatatgagc
     1201 ttagcgccct tgaatccctg cccgacatgc atatcgcact gtttaatctt gagataacag
     1261 gtacagcagc ccgtgagaca tggtccagca gtttcgtcct tccagggcat gctcccgttc
     1321 tgggaatggc cggatatacg atcgacgatg cctcgggcaa cggcaacgga aggctcgacc
     1381 cgggtgaaac cgccctcctt cagatcggcg cggtcaatga tggctcatcc ggcgctttca
     1441 atgttgaagg tacccttacc tgcgacaatg aatttgttac aattaacggt gcaacagtaa
     1501 cttacggcga cattgatgcc ggagcttccg tgatgcagtc gtttgaggtc gtcatcagcc
     1561 cggatgccga tgcgggaacc gccgcgcagt tcattttcga cctggccggc gatatgaata
     1621 ttaccggaca agatgatatt attgaatatg tggggcagat cccggttctg cttctcgact
     1681 gggacggcaa caacaactct tccgacgata tggagctgtg ctttgccaac ctggaggtcg
     1741 gatacgacaa aatggatgtg atccccgaag aacgcaatct gtatacctcc atcttcgttt
     1801 gtctcggcac ctacgacaat aatcatgtgt tatcggccga agaaggggag ttgctggcgg
     1861 aataccttga caatggcggt aatctttata tggaaggggc cgatacctgg tattacgacc
     1921 cgcagttcac ccccacgcct ctgcacgcca tgttcaatat cctgggcgtg gaagatgggg
     1981 cagatgacct ggagcagttg atagggcagc ccggttcgat tgtggaagat atgttctttg
     2041 gttataacgg tgaaaacagc tatatggacc atatcgacgc tattccgccc gcacaggtca
     2101 tgtttatgaa cgaagctcct tcttatggaa caatggtttc ttatgatgga ggaacctatc
     2161 gtaccgtagg ttcttcattt gaattcggcg gcctggtgga tggagatatg actaaagatg
     2221 accttatgat ccgtatcctc gactttttcg gagtggatgg gatttggacc ggacaggatg
     2281 aaagagcggc aggcgatatt tccaccggca gctaccccaa tccgttcgac caaacgacgg
     2341 tcatcagttt taaacttacc gaacccggtt ccgttatggt agatattttt gatattagcg
     2401 gaaggaacat tattaacctg tttgaaggac aagctacaac cggtgtgacg gagattcaat
     2461 ggaatggcag ggatcataca ggaaaagatg tcgcccccgg aatttacttt tacagggtgg
     2521 atgccggttt aagccggtcg accggaaaaa tgatgaagat cagataattc agagaatatc
     2581 ggcgttgggc tggattttcc actcgagaga gtccagttcc agcgctacca ggttgccgaa
     2641 tccttctttc ccggtcatat aaacgccatt gtcaagatcg atgaacttaa aggtgctgct
     2701 ggttctgagt accccgatga aatcaaggct gaccgggaca tgcccgtgaa tgatcttccg
     2761 gttgccggtc ttctccatat caacggtgaa ttctttgatc cagagcatcg aatggcggtc
     2821 gtcaaaggga ttatcaatac cgaaattaag gccggcatgc accaggatgt aagaattaag
     2881 ctcgatgaaa aattcgagcc cgcgcatcca ttcgatatat ttttccggaa tttcatgaac
     2941 gtcggaaacg ccgaaacttt tcagcgtctc cttgccgcca tatttgtacc actccttttt
     3001 tagctggttc cggaatgaaa tgcccagcag gttcttcctg acagtttcat tatcaaaggt
     3061 tcggatcagg taatcttcat gattgcccct cagcgtgcgg atcgtgtaat cgtcttcctg
     3121 aagccggata atgtaatcga tcaccccttt gctgtcgttg cccctgtcga tgtaatcgcc
     3181 caggaaatac agcgtatcgt aacgggtagg tttgatctgg tcttctataa gtgcctgtaa
     3241 cgttttacgg ttaccatgta tatccggaat tacccagtgc ttagccatta cctttcattt
     3301 cttcttcgtc tggttggcga ccttttccat caactggcgg atcacctcag gatcaccgac
     3361 gaaataatca ttcaataggt tgagatcatc atcaaactca aaaacatacg gaatacccgt
     3421 agggatgttg aattccagga tctcttcatt gctcatcttt ttgagatact ttacaattgc
     3481 ccgaaggctg ttgccgtgag ccgcaatcaa gacctccttc tcagccagga gcgttttcga
     3541 aatatcctcc tgccagaggg gcataatacg ggcgataacc tctttcagcg attcggtccg
     3601 ggggatatcc tcatctttta atgcagcata tcgcggatca aattttgcgt tcttttcatc
     3661 atcttccgga ataggaggag gcaatacatc gaagctgcgg cgccagagct gaatctgttc
     3721 gctgccgtat ttttcggcca tatcgatctt attcagcccc tgcaacatcc cgtaatgctt
     3781 ctcgtttagc cgccaggtct tcttctccgg tatccacatc agatccatct cctccagcgc
     3841 cagccacaac gttttgatcg cccgcgtcag gtaagaagta tatgctgttt tgaaacggaa
     3901 tcctttttct ttcatcaccc ggcccgcctc gcgtgcttcc ctgatcccct gctccgataa
     3961 tcctacatcc acccatcccg tgaaacggtt ctccttgttc cacgcgcttt cgccgtgcct
     4021 caacaatacc agtctgttca tctcttctat atattttgca aaagtataaa gatttattga
     4081 tataactttt ataacccgaa tttcaacgga gggcaaaagg cattatttta tactttagca
     4141 aaatattttc attcttaatc catttagtaa ttatggatct tatctataaa aagctgaacc
     4201 ggatgatcgg ttttatcctg ttcggcgtgg catccctggt ttatatcatc acatcggagc
     4261 ctactacctc tttctgggac tgtggcgagt atatcgccac cgccttcaaa cttcaggtgg
     4321 gccacccccc gggcgccccc atcttccagc ttttgggccg gttcttctcc ctgttcgctt
     4381 tcggcgatac ctcccaggtc gcccggatga tcaataccat gtcggccctc tccagcggat
     4441 tgaccattgc ttttctcttc tggaccatca ccatgctggc aaagaaactt ttggcgaaaa
     4501 tggaaatgac ccgggctaac ctgatcgcta ttttcgggag cggcgttgtc ggtgcgctcg
     4561 cctttacttt cagcgactcg ttctggtttt cggccgtcga aggggaagtg tacgccatgt
     4621 cgaccttatt cactgcaatg accttctggg ccatactgaa gtgggaagca gtggccggag
     4681 agccctattc ctaccgctgg ctgatcctga tcgcctacct tatcggtgtc tctatcggcg
     4741 tccatttact gaacctgctg gctatacccg ccatcacctt cgtcttcttc tataaaaatt
     4801 acaacatacc cgggtggaaa gggtttttcc tggcgctggg catctctttc ctgctggttg
     4861 ccctcatcct ttacggcatc gtgcctgaga tcgtctcgct ctttgccaac accgagctgt
     4921 tctttgtcaa cacgctcgga ttgcccttca actccggcac ctacttcttt atcctgctcc
     4981 tggtcgcact gatcgttttc ggattgcttt acacccactc cgacaacaaa ctgtatccca
     5041 ggatcatcat cgggctgggc gccgtgaccg tcgtcctctt catgctcgaa acgagttccg
     5101 ccgggaactt cttcggccgc ctgatcgctg cctcagccgt ggtcggcctg ttctacatgt
     5161 ggcgcaaccg gaaagccctg ctgaacaaca tcatcatcgc ttttgtcttt atcctggtcg
     5221 ggtacgcctc ctttttcacc ctcgtcatcc gttccaacgc caatcccccc atcgatgaaa
     5281 ataacccgga aaacgccatc agcctgttgg cttatcttaa cagggaacaa tacggcagtt
     5341 tcccgatttt ccacggccag tactacaatg cgccgctaat tgacagggaa gacggtaaac
     5401 cggtgtacaa aagagacgac gcctccggaa aatacattgt ctgggacagc cgcgaaggga
     5461 cagttccggt ttatgacccg gagtacacaa cgattttccc gcgtatgtgg aacaaccagg
     5521 aagaccggta tatccaggat tacaaaaact gggcgggtat ccgcaacgat ccggataaca
     5581 agcacatccc caccttcagc gaaaacctga ggtatttctt ccgttaccag gttaaccaca
     5641 tgtatatccg ttatttcttc tggaatttcg tcgggcggca gaatgacaac caggggatgt
     5701 atgccgacct gctcaacgga aactggatca gcggaatagg attcatcgat aaaatgcggc
     5761 tcgggccgca ggatgtcccc ggcagcatgt cgaacgaagc ccggaacacg ttttttctgc
     5821 ttcctttgct ggccgggatc ttagggctcc tttaccagtt cgggaaaaat gcaaaagacg
     5881 ggacagttgt gttgctactg tttattatga ccggcctggc cattacgata tacctcaacc
     5941 agcattcccc gcagccccgt gagcgcgatt atgcctatgc cgcctcattt tacgccttca
     6001 cgatctggat cggcctgggg gttctctggc tctatgagtt gttgcagaag aaactgtcgc
     6061 acaaaatcgc ggccataacc gcttcggcgg cgatgttcct gcttgtgccc gtgatcatga
     6121 tggcccaggg atggggcgac catgaccgct cggggcgcta taccgcactc gaagtggcaa
     6181 agaactatct caactcctgc gaaaaaaatg ccatcctgtt caccaacggc gacaacgaca
     6241 ccttccctct gtggtacgca caggaggtgg aagggatccg gaccgatgtg cgcgtctgca
     6301 acctgagcct gctgaatacc gactggtata tcgaccagat gctgcgaaaa gcttatgatt
     6361 ccgatccttt accgttcacc cttcccgaaa cgatgtacaa aaacggctcg cacgacgtga
     6421 catacctgct ggaggaacaa aatcccaaca aggagttcat cgaagtgaag gcgatcttcg
     6481 accggttgaa gaagaatgag aagatcttcc aggtgaatac cgggcgcggc atggctgatt
     6541 attttccgac caagaccttc aggatcactg tggatccggc ggtggcggtc tcttccggaa
     6601 cagtccccgc agagctggcg gggcaggtga ccgacctgga gtggcggatc agcgccaacg
     6661 ccctcaccaa gaactacctg atgatgctcg acctgctggc ccataacaac tggcaacggc
     6721 ccgtctatta tgtcagcacc accagccgcg atgcctatat cggcctcgac aactacctcc
     6781 ggctggaagg ctttgcctac cgcctcgtcc cgatacggca gacggtcgat aaaaatgaaa
     6841 ccggcggcgt caataccgat gccatgtatg ataacctgat gaaccggttc gagtttaaca
     6901 tcaacaagcc cggattactg gtcagcgaag atatctaccg gatgaccgtg acgatgcgca
     6961 gcacctattc ccggctgatc gatgccctga ttgcggagaa taagccggca agggctgtag
     7021 aggtctgcga ccgtatccag acgctcattc cgggcaaaaa agtcccttac aactacttta
     7081 acctgtcggt ggccgatggc tacctgaaag caggcgaagc ggacaaaggg cgtgaaatcc
     7141 tgcaggaact cctgcggata caggaagatc agctgggcta tttctttagt tttggcgata
     7201 agcatcttcc gttgctggag atggacatac agcagggggt ggcggtgttg agtgcggtgg
     7261 cccaggtagc cgaatcaaac ggccaggatg acctcgcaga ggaggccgac gcttcgctca
     7321 accgttttta tgaactgtat ctcggcaggg tcggaaactg atacggcatc gcattgaaag
     7381 accagctact gaccggcatt ttcacctatg tgctcccatc agctttgctc tggcaggtaa
     7441 aaacggccga aaaagcggtt tacctgactt ttgacgatgg ccctatccct gagctgaccg
     7501 acgaaatcct gggtattttg aaacaatttg atgcgaaagc cactttcttc tgtgtcggag
     7561 agaatgtgaa aaggtacccg gaaatctttg ccaggattct gcaggcaggc cattcggtcg
     7621 gcaaccacac gcaccatcac ctgaaagggt ggaccacaaa ctacaacgac tacatcgcgg
     7681 atataagcga agcggccgga tacatcaatt ccggcctttt ccggccccct tacggcctga
     7741 tgacctaccg gcaggctaaa atactggcga aggattacca ggtggtgatg tggtcagtgc
     7801 tgaccaaaga ctacgatccc tccgtgtcga aggaggggtg cctggaaacg gctatccagg
     7861 ggatgcgtcc cggtgcgatc atcgtattcc atgataacgt caaagcgaaa gagaaggtcc
     7921 tttacgcgtt gccgcggctg ctggaacgga tgaaagagga ggggtttgtt tcggaaaggt
     7981 tggtaaatgg cgatggcgat ccttcctcga tggtctcatc tggatgaagg tatccgaatt
     8041 gcctctcatc gctcctatat tttgcgaaac aaagagactt tctgtcataa tgtcgttggt
     8101 taagataatc gtcaccatct aatgttactt atgacagcct ggatattgcg tacgatagct
     8161 gattaacagt tagtggctgg cctgcaattt ttctttgcgt tttataccat atttttttct
     8221 aatggcagca agtctttttt cacgtaaaga aggatttttt tcatattctg tatgatgttt
     8281 atccctgatc ttgcgcatta attttacaca gtccaaattt tcagtactca ttttcctcaa
     8341 attttacaat ttcacgcggg cttctgattt ccagtaaaga atatccattt tttaaattaa
     8401 cagcattata ctgccggata cgctccaggt taacgatatg cttaaagttc cagcttgcaa
     8461 gtacagttgc tttgttaatt gttgccagtg caatatgtaa agcatcttca aaaaatttct
     8521 cggaaactgt accttctgaa atataaaatt cggctagttc ccgagtttcc tggtcagcag
     8581 caatcaattc cttgaattca cttttcatga tttcatccag tttttcctgc actgattttg
     8641 gcgcctcctg gatttcatca attgttatat cagagatgat ggcaaccttc tggccggata
     8701 ataattcctt aactaaaaga ttcgaccagg tcttgaattc ctcatcaaag cacccgccaa
     8761 taacagacgt atctatgtaa accctgtaag ccataattta aattcaaaga taataacaaa
     8821 acggtaagat tcctaaaaca gcagattagc cacaagcgct gttcaaattt aaaaatgaaa
     8881 tctttatgtc aagtgtcttt ctgccacaaa ggcccgaagg caccaaggat cacaaaaggt
     8941 cgtggggaga cggtagggca ggatcaacaa aaccataact gacgggttta cctgtcggat
     9001 tgttattgtt ttacgaacct ttcgatgagg gtacgattct gcatttggat tttaaggata
     9061 taaattcctc tttgcaaatc agatatatca atcagactgg aatttgagtc catgtatttt
     9121 ctgaacacta atcttccgga gatgtcaatt atctcagccg ttgagccggt agataaatta
     9181 ccgatattaa ggttgcttat agaaggattt gggtatatta acaatgaact gacatcagct
     9241 tcactgatgt tcaaaggagg atagttgtaa aattttgcca ggccgccaaa tgttcccagc
     9301 cataaacatc ctgaactatc tctggctatg gaatagaagc tgttataagg caagcctgac
     9361 agggaaggaa caaatatttc ccatgtctta tcatcaaatc tggcaaaccc gttgacagtc
     9421 ccaatccatt tactgccatc atcattcaac aaaattgata tgacctggtc atccggaata
     9481 ccggaattag actttctgta atttacccat ttttctccat caaaagtagc caggccaaat
     9541 tcgtctgctc ccatccaaag ttttcctgaa ttgtcggccg tcaaatcaat tacccagtta
     9601 gacggtaaat cggaattaaa tacggtaaag ttagtcaaga cctgatctgc aagtttatag
     9661 aggccattgc cataggtccc tacccataaa ttgcctgaat aatccatgca gattgagctc
     9721 acccaaaata tatcggaaat tgtccactga ttgtcgtcaa aaagggcaag gccatcctta
     9781 gttccaaacc actttcgccc gagccggtca ataaagattg aatagacaat gtttgatggt
     9841 aagccggaat tggatgtatt atacaccgtc cagttgtttc catcaaatac agcaacgcct
     9901 ccaaacgtcc cgatccagat atttccaagg ctgtcgatta cgattttatt tatcgtatcg
     9961 gaaggtaaac ccgaatttga cctgtagtaa tttgtccatt tacttccatc gaaactggaa
    10021 agtccgtttt tataggttcc gacccattta ttaccggaat gatcaatggc tactgagcta
    10081 atccaatagt aaggcaggcc ggtatttgca tgatcgtata aagtccagcc ggagccatcg
    10141 aattgagcga tacccgcccc aagatagtac ccagcccaat tgtctgtcag aatactgatc
    10201 cagttattgc cattcgtgtc aatacaaaca gcatttactc tatcatcggg cattgcagta
    10261 ttggaactgt taaacactgc ccaggtctcg tcatcgtata ggaccagccc cgctgttgtg
    10321 ccgatccatt tattttcaaa ttcatcaatg aatattgaat agatattatt tgagggtaaa
    10381 cctgaatttt cttttgtata aaattgccag gtaagatcat taaattttgc tattccatct
    10441 ccgccaaaac aatgtccgga ttcttgattg gtaaatgaga atccaatcca tttatggttg
    10501 tacttatcga tcgatatgga acttatcgaa tctgagggca gatcagagtt ctccgcattc
    10561 aggagcgtcc attcggaacc atccagcttt gctaatccgg catatgtacc gatccattta
    10621 gcgccggatt cttcaatggc aagagcagtg atatagttgg agggtaataa cgaattttcg
    10681 gaattataca gggtccaggt tgtatcatta aaacacagga tcccgcctct aatgtacccc
    10741 tggttcattt catattttgg tgtaatgcca aaccatttat tgttcagatg atcgattgca
    10801 attgttgaaa tcgtatcaca tggcaggcct gaattcgatt tattatacac aatccagtcg
    10861 gccccgttat atttcacgat cccgcccgta gtgccaagcc atttgtttcc aaatttgtca
    10921 atggttatgg cataaatcat atccgatggt aacccggagt tcaaaatatt ataattggtc
    10981 caggttgttc cgtcaaatcg tgaaagtccc gaccttgtgc caaaccacat agttcccatg
    11041 ctatcggttg ccacagaaaa tacatgatcg gaaggcaagc ccgtattttc aggtgtgaaa
    11101 aactcccaat tctgtgcatt aaaatttaaa acaaataata cactgataag gagaagggaa
    11161 atgtttttca tttcagtgat atttacagca ccttctttac cgaatacagc ggcttcttgt
    11221 tctgaaccat atatatccgg ctgagatatt ccccgataac cccgagggaa agcaggatca
    11281 ggctggtgga gaataggatg gcaacgatca gcgaggtgta tcccagcgga acgttcatca
    11341 ggatcttcct aaggataaag tagatggaga agataaaaaa gatgacagat gataccattc
    11401 cgccgtacac catcaccttc agcggggcag tggaataatg gtacatcacc tcggtgaaga
    11461 accgccagat cttgccccgg ctgtaccccg accgtttgta tttccgtgga tggtgctcca
    11521 cggtggcgta accgatgaaa tcggtatacc agtgcagaat ttcgtcgatg aaaacgaaat
    11581 tttgatggtg tcccaggatt ttttgtatga tgctgccgga gatcagccgg aatgatgatc
    11641 cctccgtctg gcttccgaac agccgtgaga ggctcttgaa cgaccggctg ctgatgttcc
    11701 tggccgccga atgccgcttc ttccggtaga tgccgtaaac cagctcggca tcagtctctt
    11761 ccatcgtacg gatcagcttg ggtatctctt cgggcgggca ttgcaggtca tcgtcgaggg
    11821 tgatgatatg ttttcctttg gcaaagctga aaccgcaaaa tgtggcgctg tgctgcccga
    11881 agttcttcgc cagccgtatc gccgtaaatc catcggggtt ctccgccttg atctttttca
    11941 gcatctccca gctccggtcg ccgcttccgt catccacgaa gatcatttca tacggcttgc
    12001 ccgtcgcctt cagaacctcc gtcaggcgat ggaaaagttc ctccagcgac gcttcgctgt
    12061 tgaaaaccgg gactacgacc gaaaattcga tttcattcac gcggatgatt tttcacaaag
    12121 gtagcaaaga gatgaaataa ttaaaaattg aaatttgaaa attgaaattt aaaggaaaat
    12181 ccttgataag taggaaaatt cgtaatacct ttcggcaact attactcctc tctgaaatca
    12241 ctaataaaag atgatcgggg atcatctgca gaccaaacct gtaatctctc atgttataaa
    12301 tactaattat tgattatcat taaattaaag attatgaaaa caaccaaaat cttgatgtgc
    12361 atcttcgtga tgcttgtttt tattggtctt aacacaacaa aaggccagtg gctctacaac
    12421 gggaccagta tttacaattc taactcaggc aatgtgggta ttgggaacaa ttcaccggga
    12481 tcactgcttt atgttgcaaa gaacatgact gaacctacga tcactgttcg taatcttggc
    12541 ggcaccggtg gcgctgcgta taccatgact gatgatgcca gcggggcgaa ttggaagttc
    12601 aaagcaacga acaacggggg ctttaagatc cgtgaccatg caaatctgct tgatgtgata
    12661 accattgagc ccaacagcac ggcaaatgcg atctatatca atgccgaagg caacctgggc
    12721 gtgagaactt caactccggc tgccaatttt catgttgcag aaaataatcc aagctacacg
    12781 gctgcttttg gaaatatgat cagtggctgg agtgacggta ctaatgtgag cattgggaat
    12841 gatgatttta attcaatctt atatgtcggc caggatatga gtcataaagg atatcttatc
    12901 tggaaccatc atcccgaacc agacgctgct actttcatga ttggtactta tgatgggact
    12961 aatccaatga tactccaacc tgcaggcgga aatgtaggaa ttggcacatc tacgccttat
    13021 gcccgcttag atgtcatgaa caatttatat gattacagca gaattgggaa tactgcgact
    13081 gtcgcaaatg agtttcttaa ttatgaggaa acggccacag tggatggcca atgcgcaatt
    13141 atgacggtga ggcatcgtgc tgaacaaaat gatggggccg cctataatat aggtcaaacc
    13201 aatgcgagta taataggtta taatgtttgg ggtgatctgt ttacgttcgg gtcaatagga
    13261 tataactata acgattttac ccgatgtggc ggggcc
//
LOCUS       DAOPNH010000056        12942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_4961_length_12942_cov_8.122294, whole genome shotgun sequence.
ACCESSION   DAOPNH010000056 DAOPNH010000000
VERSION     DAOPNH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4961_length_12942_cov_8.122294"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKZ74_04500"
     CDS             <1..191
                     /locus_tag="PKZ74_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM92050.1"
                     /translation="FEPFRRGQNAYPVKGHGIGLSLVERIVILHNGSIEVDSQPGEGT
                     TFRVFLPQKTDPEFLTTF"
     gene            413..1282
                     /locus_tag="PKZ74_04505"
     CDS             413..1282
                     /locus_tag="PKZ74_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92051.1"
                     /translation="MKMNRERVIAATTTILLVASLIGTGYYYRTSGTLKSGLNNEKLK
                     SESLLSEKLALAKEIEKLKSDISQWQGKSQESDKLLAEAREKIEAMEKTMSWLRKENA
                     TVASLKKELNELKQLRTDLEAQLAELERKNKDKDNQIEALHAEVIALKSERDGLKSDV
                     AALKANITDDFRVETLRGKKNQKLTLNARKTKKLMVSFEIPQSMSDNVRFTIETPNGK
                     EIKSDDKSLTYNVVDDGRNMIANLSPYTGEFEISRRIEMAYKPDQKLEPGIYKIKIYH
                     KDTYTGSCQVRLK"
     gene            1451..2587
                     /locus_tag="PKZ74_04510"
     CDS             1451..2587
                     /locus_tag="PKZ74_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92052.1"
                     /translation="MRLLTVSILASLLLLSCATGKKAYERGDYFNATIQAVNRLRSNP
                     GSEKAQEALRRSYPAALGYFQEKIENALRSNRQFRYSEVVDYYVMMNHMADEIRRCPA
                     ALSLFPQPKSFESELAEAKYKAAEEHYQAGLDQEKLNTRTSWKSAYLNFNEADCFVPG
                     FKDAKERMRIAYLNATWKVIVEPIPVPNAYQLTSDFFRNQVIEYLRNERPNEFVEYYT
                     PESAEKAGIKKPDQVLRMSFDQFVIGQVYDKETITELSRDSVVTGTVRLPDGTEHKVY
                     GTVKAKLRTFRREVISNGVLDVSVIDWPLNQVSTERKFPGQFIWYIEWATYNGDERAL
                     SKEQLALCNKVPAPPPGPQNLFIEFTKPIFRQVSPYLQSYFSSY"
     gene            2870..4090
                     /locus_tag="PKZ74_04515"
     CDS             2870..4090
                     /locus_tag="PKZ74_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92053.1"
                     /translation="MVQNGLIEELIKLDSLSKEQAGKYPLKRFLFPNLKFLLTNTRQI
                     VGIAGLRGTGKTILLRQLAVDLGQSFYLSADTLPLGTDLFDLSARLAGTFGIKYLMVD
                     EIHALKDWQGQLKKIYDFQEIRIIFTSSSSLELSEGRYDLSRRLTLTQLPLFSFREFL
                     FFKNNTSLPVIPLEDILLKHKKIYQDLYSFEPQFRNYSGIGALPACLDSPLPSIITST
                     VDKVIQKDMLTVGRLSMEDIHSIKTVLLFVARAGIEGCGYSSISRNTGITKYKAQQYI
                     EMMEQASLLKIVLPYGANVTPEPKIMLVPTLRMNLAQGVDDDRLTGAMREEFFIHHII
                     GAGLTVNYLKSMRGQKLPDYMVFFKDQKLIIEIGGAGKGASQFKGIEGKEKLILTQPG
                     SPNGIPLILFGFLW"
     gene            complement(4166..5206)
                     /locus_tag="PKZ74_04520"
     CDS             complement(4166..5206)
                     /locus_tag="PKZ74_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPM92054.1"
                     /translation="MENLAHTFKLNLNWKLISRISQIDRFDASWSSIEKREGQSLTQL
                     KSVATVRSVGASTRIEGSQMTDEEVEALLKEIDITKLHDRDSQEVAGYFEVLDIISES
                     YQDIMISESSIKNLHNILMKYSTKDDWHKGEYKQHSNVVEATLPDGTSQVIFRTTDPG
                     YPTQDAMRQLIDWYNNDHQTHPLVKCALFAYDFVSIHPFQDGNGRLSRLISTLLLLKN
                     GYKWIEYVSFEHEIENRKTEYYRVLRGCQAQRPNEDVTEWVNFFFDALVNIQNQLMKK
                     LEIQGTEMMLSPREKSILTFIGNHPGCKSGEIARKLKIPGPTVKRILPVLIEKKLIEK
                     FGTGPGTNYSLK"
     gene            5851..6654
                     /locus_tag="PKZ74_04525"
     CDS             5851..6654
                     /locus_tag="PKZ74_04525"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HPM92055.1"
                     /translation="MKYALVDNNKTEATKGVTGICPGCGSELIAKCGDIKLHHWAHKG
                     NRKCDPWWENETPWHRSWKNNFQSEWQEVILCDEQTGEKHIADIRTTHGLVIEFQHSH
                     INPQERTSRENFYKNMVWVVDGTRLESDYKRLDKGKNNFRKTNTKGLYLVSFPEECFP
                     SNWLKSSVPIIFDFRGTELNIDMNDMRNSLFCLYPKRLGQKSILVTMAHDFFIKNIIK
                     GEWQILMNNVSQFQQAWQKVLDLQQRQQTNFVFDKLGSAIRYKNRRRRF"
     gene            6765..7319
                     /locus_tag="PKZ74_04530"
     CDS             6765..7319
                     /locus_tag="PKZ74_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92056.1"
                     /translation="MDAVTLTWTGITSLLVSSGVTYFLHFKIVKHIGSELTKLMTIFH
                     QNEEVLKKIELQNDKAVKALELQNTKVITKWEYENERLLKKIDRGIEVKERSQVVAEL
                     FTLWMQTADGPSHRDLSPDEFAKMNRYSFECALWLPQEIFHELSKTLENAEGHLHYKE
                     ILRQIRMYLNPELGDIDAGRIIQW"
     gene            complement(8098..9840)
                     /locus_tag="PKZ74_04535"
     CDS             complement(8098..9840)
                     /locus_tag="PKZ74_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92057.1"
                     /translation="MIKVDHTKIMEMFCETDLLGLIRQINTTTRNSGMEENMMIAAGK
                     AAEALAGRLNCSPRQAVIFSIVFNLNFNTELITLTELAGFLSCEPIDIAAYTRDLEAL
                     ADLRLIKRDRVDRRMFDYRVNNLSRYYVPSPIIQSVCEKNEVLMKESSPETLNELLVY
                     LNELLDSSQGMERLLIDLSLEVTSLILDNHDLPFARTLISQELGENSLLIVCYLSLAL
                     FNGHESTGLAEMLKEIVPDARIQFAIKRQFEKEEHELLKKELVCFVAASFKGDLEVRL
                     TEKFAAKLFEGEQDLLKPDRSRKNSGLILYDMIPEKQMFYSIEAKRDLQFLEEALLPD
                     NFGKLMKKLQDRGLPQGMNVLLYGPPGTGKTETVFQLARRTGRDIRQIDISETKSHWF
                     GDSEKLIKQIFDQYRYVVDKSTTAPILLFNEADGIFTRRKATGKSSTDQTENAIQNII
                     LQEMENLRGIMIATTNMAGNLDKAFERRFLYKILFEKPDMEARLSIWKTRLPELDKEI
                     IKHLSERFDFSGGQIDNIARKYVMHHILRDKDPFLHDILAWCREEGMQEGNVRIGFRV
                     QTLTDTAGAERCQD"
     gene            complement(10022..10291)
                     /locus_tag="PKZ74_04540"
     CDS             complement(10022..10291)
                     /locus_tag="PKZ74_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92058.1"
                     /translation="MGTTLQGLDNLDSIGGDLQLGTTSWLADLKGLNSLFFIGGDLSV
                     DYNESLADISALSSLTCLHGDLERYNNDVLTTPSGLKGRLPFSVR"
     gene            complement(10249..10467)
                     /locus_tag="PKZ74_04545"
     CDS             complement(10249..10467)
                     /locus_tag="PKZ74_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92059.1"
                     /translation="MLSRRLIVFGFGLFAGIGCYSQSCLPEGIEFVRQGQVDSFPLIY
                     PGCEVIEGNIYIGGLWEQPCKDSTTWIR"
     gene            11221..12423
                     /locus_tag="PKZ74_04550"
     CDS             11221..12423
                     /locus_tag="PKZ74_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPM92060.1"
                     /translation="MIVFLDTVRERKKGTSSVRIQIIYRRYPKFYSTRIQMLPEDWLK
                     MLGLKPRGDLKEKKIIIYEMLRKVLTIIYGMEEFSFEGFEKLYIGEEKTKDDVYAAYD
                     RYIKELKESGRLGSENFYAYSRDSVKKFAGDKRLSFKSINIQFLKKYEAHMLLKGDAL
                     TTVAMYLRCLRFLYNNAIKEGIVRQQDYPFGRGKYEIPQPQNIKKALKLSDIGKIFHY
                     KPESEQESYYRDIWMFSYLCNGINLKDICLLKYSDFQGDHIYFRRAKTINTNRNSKPI
                     DIIVTDEVRQIIDRWGNKPVQPDSFIFPVLTNDLSAEKKHADIAQAVQMTNRYIRRIA
                     KKIGIEANVTTYTARHSFATVLKRSGVNVTYISEALGHSDIKTTENYLDSFEDEQKKE
                     IAKKLTAW"
     gene            12515..>12942
                     /locus_tag="PKZ74_04555"
     CDS             12515..>12942
                     /locus_tag="PKZ74_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92061.1"
                     /translation="MVQNGLIEELIKLDSLSKEQAGKYPLKRFLFPNLKFLLTNTRQI
                     VGIAGLRGTGKTILLRQLAVDLGQSFYLSADTLPFGTDLFDLSAQLAGTFGIKYLMVD
                     EIHALKDWQGQLKKIYDFQEIRIIFTSSSSLELSEGRYDLS"
BASE COUNT         3759 a         2894 c         2793 g         3496 t
ORIGIN      
        1 ttttcgaacc cttccggcgc ggccagaatg cttatcccgt caagggccac ggcattggtc
       61 tctcccttgt agaaaggatt gtgatacttc ataacggatc cattgaagtc gattcacagc
      121 ctggcgaagg cacaactttc cgggtctttc tgccccaaaa aactgatcct gaatttttaa
      181 ccacatttta atcccggttt cattccgttt ttatcctgca gtaatagttt tgcttccaga
      241 tttagtgtta attgggacta aggagccgct tatcctgaaa ccagcatgaa atgaatgaag
      301 cggtaaaatc cattaaaacc aacaagaact acctgatcgt tggcatgctg gttttctttt
      361 acattgaatc agaaaaacaa ttattgtata atcatttaaa acaagcatgg tcatgaaaat
      421 gaacagagag agagtgattg ccgctactac aaccatactt cttgtagcat cactgatagg
      481 aacgggatat tattaccgca ccagcgggac gttgaaaagc ggtctgaata acgaaaaact
      541 gaaatcggaa tccttgctgt ctgagaaact ggcactggcc aaggaaatcg aaaaattgaa
      601 aagcgatatc tcacaatggc aggggaaaag ccaggaatcg gacaaattgc tggcagaagc
      661 cagggaaaaa atcgaggcga tggaaaaaac catgagctgg ctcagaaaag aaaatgcgac
      721 ggtagcctct ttgaaaaagg agctgaacga actcaaacag ctcagaacgg acctggaagc
      781 ccagctcgcg gaactggaaa ggaaaaacaa agataaagac aatcaaattg aagcattgca
      841 tgccgaggtc attgccctca agtcggaacg cgacggactg aaatcggatg tagccgcttt
      901 aaaggcaaat attaccgacg acttccgggt cgagaccctc aggggcaaga aaaatcaaaa
      961 gctgactctg aacgccagga agaccaaaaa gctcatggtc agctttgaga tcccccagtc
     1021 gatgagtgat aacgtaagat tcaccattga aacaccaaac ggcaaagaga ttaaaagtga
     1081 tgacaaaagc ctgacctaca atgttgtcga tgacggacgg aacatgattg ccaacttatc
     1141 gccttatacc ggtgaatttg agatctccag gaggatagaa atggcataca aaccggatca
     1201 aaaactggaa ccgggtatct ataagatcaa aatctatcat aaagatacct ataccggaag
     1261 ctgccaggtt cgtctgaaat agaagaagcg tatatctcaa tatattttca tggtaaattt
     1321 tctcaccccg ggctaccgct gaatcccagc cggtggcccg gttttttcat ggcacgattt
     1381 tttcaatgtt caacccaagg atttcaccca aaataagaaa atttgcttac tttgtaaaaa
     1441 ttgccgaatt atgagactat taaccgtttc catcctggcc agtcttttgt tgctctcttg
     1501 tgcaacgggg aaaaaagcgt acgaaagagg agactatttc aatgccacta tccaggcggt
     1561 gaaccggctg cgcagcaatc ccggatcgga gaaagcacag gaggccctcc ggcgcagcta
     1621 ccccgcagcg ctcggatatt tccaggaaaa gatcgaaaat gcgcttcgat ccaacaggca
     1681 gttcaggtac tccgaagttg ttgactatta cgtcatgatg aaccatatgg ccgatgagat
     1741 caggcgatgc ccggccgcgc tgagcctgtt tccccagccc aaatcttttg agagcgaact
     1801 ggccgaagca aagtacaaag ccgccgaaga gcactaccag gccgggcttg accaggaaaa
     1861 gctcaacaca aggacatcct ggaaaagcgc ttatctcaac tttaatgaag ccgattgttt
     1921 tgtccccggc ttcaaagatg caaaagaacg gatgcggatc gcctacctga atgccacctg
     1981 gaaagtcatc gtcgagccga tccccgttcc caatgcctac cagcttacct ccgatttttt
     2041 ccgcaaccag gtcatcgaat acctgaggaa tgaacggccg aatgaatttg tagagtatta
     2101 cacgcctgaa tcggccgaga aagcgggaat caaaaagccc gaccaggtat tgcggatgag
     2161 cttcgaccag ttcgtcatcg ggcaggttta cgataaggag accattacgg agctgtcgcg
     2221 cgacagtgta gtgaccggca ccgtcagact gcccgacgga acggaacaca aagtctacgg
     2281 cacggtgaag gcgaagctgc gcacattccg cagggaagtg atctccaacg gggtgctcga
     2341 cgtctcggtg atcgactggc ctttgaacca ggtatcgacg gagcgcaagt ttcccggcca
     2401 gttcatctgg tacatcgaat gggccacata caacggcgat gaaagagcgc tgagcaaaga
     2461 acaactggcc ttgtgcaata aagtacccgc tccgccgcca ggaccgcaaa acctgttcat
     2521 cgagttcacc aaaccgatct tccggcaggt atcgccgtac ttgcagtcgt atttcagcag
     2581 ttactgaaag aagacgcctg cattcttatc tgcgtaaatc tgcgctaccc atttgcgaaa
     2641 atctgcgaga aattttttac cgccgcaaat cgattcatgt tccgttttgc attttgtgtt
     2701 ttgcattttt catggcattt ggcatgggga acagacgaac aagcgaacag acgaacctgg
     2761 aacgctggaa cactggaacc ctcattctgt aaatattttt accagaatgc atatcatttt
     2821 gaaaattatt ttaccaaaat gcataacttt gacatgaaaa ttattctata tggttcaaaa
     2881 tggcttaatt gaggagttga tcaaactcga cagcctttcc aaagaacagg caggtaagta
     2941 tcctttaaaa cggtttcttt ttcctaatct gaaattcctg ctgaccaata cccggcaaat
     3001 agttggtatt gcaggcctca ggggaaccgg caagaccata ttactgaggc aacttgccgt
     3061 agatctgggc cagtcatttt atctcagcgc agatacattg ccgcttggga cagacctatt
     3121 cgatctttct gcccggcttg ccggtacatt tggtattaaa tacctgatgg tcgatgaaat
     3181 tcatgccctg aaggattggc agggtcaact gaaaaagatt tacgatttcc aggaaatcag
     3241 gatcatcttt acctcatctt cttcgctgga gctgtctgaa ggaaggtatg atttatcaag
     3301 gaggttaact ttgactcagt tgccgctctt ttccttccgg gaatttcttt ttttcaagaa
     3361 taacaccagc ttgccggtaa ttccgcttga ggacatttta ctgaaacata aaaagattta
     3421 ccaggacctc tattcttttg aaccgcaatt ccggaattat tcaggcattg gggcgttgcc
     3481 cgcctgcctg gactctcccc tgccctcgat tataacatcg acagttgaca aggtcatcca
     3541 gaaggatatg cttactgtag gcaggttatc aatggaagat atccattcca tcaaaactgt
     3601 actgttgttc gttgccaggg cagggattga aggatgcggt tattcatcca tttcccggaa
     3661 tacggggatc acaaaataca aggcgcagca atacattgag atgatggagc aggcatcact
     3721 cctgaagata gtattgccat acggggccaa tgtcacacca gaaccaaaga tcatgcttgt
     3781 gccaaccttg cgcatgaacc tggcccaggg agtcgatgat gacaggctga ccggcgccat
     3841 gcgtgaagaa ttcttcattc accacattat tggagccgga ttaacggtaa attacctcaa
     3901 gagcatgcga ggccagaagc ttcctgatta tatggtcttc ttcaaagacc agaaactgat
     3961 catagaaatc ggcggggcag gcaaaggcgc atcacaattc aaaggaattg aaggaaaaga
     4021 aaaactgatc ctgacccaac ccggttcacc aaatggaatc ccactgatcc ttttcggatt
     4081 tctctggtaa caagagctta gagcattgag cgaagagctt ggaactatgg aactctggaa
     4141 ccttggaact ctggaacctg gaacattatt tcagtgagta gttcgtcccg ggtccggttc
     4201 caaatttctc gatcaacttt ttctcaatta atacaggcag aatcctcttt actgtcggcc
     4261 ccggtatttt taatttccgg gctatttcac ctgatttaca acctggatgg tttccaataa
     4321 aagtcaaaat ggatttctct ctgggtgata acatcatctc tgttccctgg atctccaatt
     4381 ttttcattaa ctggttctga atatttacca gggcatcaaa gaaaaaattc acccattcag
     4441 tcacatcctc gtttggcctt tgtgcctggc atccccttaa tacccggtaa tattcggtct
     4501 ttcggttttc tatttcatgc tcaaaactta catattcaat ccatttatag ccattcttca
     4561 ataacagtaa ggtcgaaatc aatctgctta acctgccatt tccatcctga aagggatgaa
     4621 tactgacgaa gtcataggca aacagggcgc atttcaccaa tggatgagtt tgatggtcat
     4681 tattgtacca gtctattaat tgtcgcatag catcctgcgt aggatatccg ggatcagtcg
     4741 tcctgaaaat aacctgactt gtcccatctg gtaatgtagc ttccactaca ttactatgct
     4801 gtttatattc tcctttatgc caatcatctt tcgtgctata tttcatcaga atattatgca
     4861 ggtttttaat actgctttcg ctgatcataa tatcctggta cgattctgaa ataatatcga
     4921 gtacctcaaa ataccctgca acctcctgcg aatcccggtc gtgaagcttt gtaatatcta
     4981 tctctttgag taaggcttca acttcctcat ccgtcatttg agatccttcg attcgggttg
     5041 aagcgccaac actgcgaact gttgcaacag atttcaattg cgtcaggctc tgcccttctc
     5101 ttttttcaat cgaagaccag gatgcatcaa agcggtcgat ctgactgatc cgactgatta
     5161 atttccagtt gaggtttaac ttaaatgtat gtgcaagatt ttccatactg caaaaatacc
     5221 aaaacatttt aattagtgat acgatatgat acgacataat acgataaata tccgatcatc
     5281 ctgatacgat atgatccggt ttgatacgac aatgatacaa ttattgacgg aaatgcgggg
     5341 aggcttggag gcttggaggc ttggaactct ggaacaattg aacaattgaa caattgaaca
     5401 attgaacaat tgagccatca agttatagaa ccaggggagc aacttgcctt ccctcctgcc
     5461 caaatgtatg ttcgtgtttt ataggtcgtt gacttttttt gagtaaaaac agggcgaaaa
     5521 cgccttaaaa acttcgttct ctttgataaa tgagactcaa ttttcaaaag cgcagtgcat
     5581 tgaaaattga agtcgaatta tcccgttccc tcgggattac caacgacata gataaagaag
     5641 caaagagaaa ccgtataaaa aagcaattcc gcatccgcag aatggcaatt ttgggccctt
     5701 gaaatagatt gtcattgctt ctataatttt ttacagtgaa tttttttaaa tttatacctt
     5761 cggttgtgta aaataatctg cgactactta tctgtctgat agagagatgt ttgcaagttg
     5821 gtatagaaca attttaattt aagctaatag atgaaatatg cactcgttga taataataaa
     5881 actgaagcta ccaaaggcgt tacgggtatt tgcccgggat gtggttcaga attaattgcg
     5941 aaatgtggag atattaaatt acaccattgg gcacataaag gaaatcgtaa atgcgaccct
     6001 tggtgggaaa atgaaactcc atggcatcgc tcatggaaaa ataattttca aagtgaatgg
     6061 caagaagtta ttttatgtga tgaacaaaca ggcgaaaaac acattgccga tattcgtacc
     6121 actcatggac ttgtcattga attccaacat tcacacatta atccacaaga gcgtacttcg
     6181 cgagagaact tctacaaaaa tatggtttgg gtcgttgacg gtacacgcct agagagtgat
     6241 tataaacgtt tagataaagg gaaaaacaat tttcgtaaaa caaacacgaa aggactttat
     6301 ttagtttcat ttccggagga atgctttcct tccaattggc ttaaaagttc agtgccaatt
     6361 atttttgatt ttcgaggtac cgaattaaat atcgatatga acgatatgag aaatagtcta
     6421 ttttgtttgt atcctaaacg gcttggacaa aaatctattc ttgtaaccat ggcgcacgat
     6481 ttttttataa aaaatattat taagggtgaa tggcaaatcc ttatgaataa tgtaagtcaa
     6541 ttccaacaag cttggcaaaa agtgttagac ctacaacaac gacaacagac gaattttgta
     6601 tttgataaat tgggcagtgc catacgatat aagaacagaa gaagacgttt ctgacaatct
     6661 aagaagaaag aagatgtatc gccgccaatt aaatgattat gattcctaaa tttttcgaga
     6721 aataaaagta aaatacgaca ttgtgaatat taataacaat tattatggat gcagttacat
     6781 taacgtggac aggaatcact agccttttgg tttctagtgg agttacttat tttttacatt
     6841 ttaaaattgt aaagcatata ggttcagaat tgacaaaatt aatgaccatt tttcatcaaa
     6901 atgaagaagt tctaaagaag attgaattac agaatgataa ggcggtaaaa gcattagaac
     6961 ttcaaaacac aaaggtcata acaaaatggg aatatgaaaa tgaaaggtta ctaaaaaaga
     7021 ttgatcgtgg aatcgaagtt aaggaaagat cgcaagttgt cgctgaactt ttcactttgt
     7081 ggatgcaaac agcagatggc ccttcccata gagatctttc accggatgaa tttgctaaga
     7141 tgaacagata tagttttgaa tgcgccttat ggctaccgca agaaatattt catgaactat
     7201 caaaaacact agaaaatgcc gaaggtcatt tacattataa agaaatttta aggcaaatcc
     7261 gtatgtattt aaatccagaa ttaggagaca tagatgctgg gaggattata cagtggtgac
     7321 tcaataagga gcagctttac tcaactgatc caactttctt taccctttgc aaatttattg
     7381 cgttgctgca tgtacttgca tttttatctt tctccgtatt ttcttaacgc cttcgatcgg
     7441 gtagaaacat tttttctctg aataatggtc tcaaacctgc ttcctgatta ttccttctag
     7501 cccatagata ttcttgccga cccttgcttg tctccggcaa gcaattttct ctgtgagcga
     7561 cctggctttt cccctgctga ttatccaggc atcactatgt agcatattgc ctagcatcca
     7621 gttccatctt gggccggagg gcttcagttt tctatctcct tcgttcatgg ttgtatttca
     7681 cggttgctcc tgctaaatgt gactattgta ttccaactct cctggtcatg aatattatgc
     7741 acaaaggtaa attcgttcaa tttcaccttt caagatcaag cctttggtac acatgtcccg
     7801 gaaaagccgg gattcacttt tgcagataaa agtgacatga aaccttgccg ggtcatctca
     7861 ctaccttttt tttcgcgcat aattcattaa caggtgagcc aggttcaaga aagattttcg
     7921 attgtcgatt ttagatggcc gatggtcgct gtgagattcc atcgaaaatc ctaaatcggc
     7981 tatcgaaaat cgaccatccc tgaaaactcc tttcggcact tcgtgttcta taatctttcc
     8041 caacacactg gttttgatgc tgtcaggacc tgcggacgct gacatgtcat attcctctca
     8101 atcctgacag cgttccgccc cggccgtgtc tgtcagcgtt tgaacacgga accctatccg
     8161 cacatttcct tcctgcatcc cttcttcccg gcaccaggct aggatgtcgt gcaagaatgg
     8221 gtccttgtcc cggagaatat ggtgcatcac atatttccgt gcgatattat cgatctgtcc
     8281 gccggaaaag tcgaaacgct ccgaaaggtg ctttataatc tccttatcga gctccggcag
     8341 acgcgttttc caaatgctga gccgggcttc catatcgggc ttttcaaaaa ggatcttgta
     8401 caggaaccgg cgctcaaaag ccttgtcgag attaccggcc atgttggtgg tggcgatcat
     8461 aatgccgcga aggttttcca tctcctgcag gattatgttc tgtatggcat tctccgtttg
     8521 gtcggtggaa gacttgccgg tggctttcct ccgggtgaat atgccgtccg cctcattgaa
     8581 cagcaggata ggcgcggtcg tgcttttgtc cacaacatac cggtactggt cgaaaatctg
     8641 cttgatcagc ttctccgagt cgccgaacca gtggctcttg gtctcgctga tgtcgatctg
     8701 ccggatatcc cgtccggtgc ggcgcgcaag ctggaagacc gtctcggttt tgccggtgcc
     8761 cggtgggcca taaagcaata cgttcatacc ctgtggcaat cctctgtcct gaagcttctt
     8821 catgagcttc ccgaaattat caggcagcag cgcttcctcc aggaattgaa ggtcccgctt
     8881 cgcttcgatg ctgtaaaaca tctgcttctc cgggatcata tcgtaaagga ttaaccccga
     8941 attcttccgg ctacgatcgg gtttcagcag gtcctgctcg ccctcgaaga gcttcgccgc
     9001 gaacttttcc gtgagcctga cctcaaggtc gcctttgaaa gacgcggcga caaagcaaac
     9061 cagctctttt ttcagcagct catgttcttc cttttcgaat tgccgcttga ttgcaaactg
     9121 aatgcgggca tccgggacta tctccttcaa catttcagcc aggccggtgc tctcatggcc
     9181 gttgaaaagg gctaagctga gatagcaaac aattagcagc gaattttccc cgagttcctg
     9241 gctaatcagc gttctggcaa aaggaaggtc atggttgtcc aggatcagtg aagtgacttc
     9301 caatgacagg tcaatgagta atctttccat cccctgagaa ctatccagca gttcattcag
     9361 atacaccagc agttcgttaa gggtttccgg actgctttcc ttcataagta cttcattctt
     9421 ttcgcaaacg gattgaatta tcggggaggg gacataatag cgtgacaggt tatttacccg
     9481 gtaatcaaac atcctcctgt ccaccctgtc ccttttgatc agcctgagat cagccagcgc
     9541 ttcaagatcc ctggtgtaag cagcgatgtc gattggctcg catgacagga atccagccag
     9601 ttcggtcagc gttatcaatt cagtattgaa attcaggttg aagacgatac tgaaaatgac
     9661 agcctgcctc gggctgcaat taagccggcc agccagcgcc tccgccgctt tccctgccgc
     9721 aatcatcatg ttctcttcca tgccggaatt gcgggttgtt gtgtttatct gccggatcag
     9781 tcccaacaga tcggtttcac aaaacatttc cataattttc gtatgatcta cttttatcat
     9841 tggctgcaga tttgcgcaac gatccgccaa tatgccgcag ccactctgca ccgggcagga
     9901 tatctgagga tgcctccagt taaggcctcc tgatatatat taatcggaaa aaatgaaaaa
     9961 aatacccggt ccgttgaaaa aatctttttt gaagatgatt ttgaccctgt atggttctcc
    10021 ttcatcgtac gctgaaaggt aaccttcctt tcaagcctga cggcgtggtc agaacatcat
    10081 tgttatatct ttccaggtca ccatggaggc aggtcagcga cgacagggcc gatatatctg
    10141 ccaggctttc attgtaatca accgacaggt cgccgccgat aaaaaacaga ctgttcaatc
    10201 ctttcaaatc ggccagccag gatgtcgtcc caagctgaag atcaccgcct atcgaatcca
    10261 ggttgtcgag tccttgcagg gttgttccca taatcccccg atgtaaatat ttccctcaat
    10321 gacttcacaa cccgggtaaa tgagcgggaa agagtcgacc tgtccctgcc tgacaaactc
    10381 tatcccttcc ggaaggcagg attgggaata gcatccgatg ccggcaaata atccaaaacc
    10441 gaatacgatc aatcttctgc tgagcatcaa tcgctttttg atttgagcat ataaatataa
    10501 aaaatatagt ccgggaatca aaagacgaac ggattaagcc aaaacagaaa caggatgaaa
    10561 tctcaggaaa gcgattcatg ttccgttttg cgttttgcgt ttagcatttt tcatggcatt
    10621 gggcatgagg aacaagggaa caagggaaca aacgaaccag cgaacaaacg aacctggaac
    10681 cagaaaccag aaactcgaaa cacgattgcc gaattccttc ttcaatcgtg tttcgagttt
    10741 cataaatcgt gcatcctgtg gagtataccg gagtcgaacc gatgaccttc ccgacccagt
    10801 cgggacaccc tatccaactt attgaagatg tggagtatat cggagtcgaa ccgatgacct
    10861 cttgactgcc agttgacatt ttaccctatc tgttgcctct ttttatcctt ctattattat
    10921 tgatattcaa tgttttaaca aatttcctta taatttattt tgtattatta ccgataattt
    10981 aattaaattt gttagttatt ttgttagtta ttttttttgt atcacttatc tgtaaaagga
    11041 cacacgatgg catcatctgg taaagctagt aaaatcaagg gaaaccgaga ccctcaattt
    11101 gtaaaaaatg ataaaaatcc ggtttcgggg gaaaataata ccccgaagaa aaaggaaaaa
    11161 ttaccgaccc tgacccatcc agaacaaaat attgacctgc ttaaaaacaa aggagtgtcc
    11221 gtgatcgtat ttttagatac agtcagggaa agaaaaaagg ggacttcaag cgtcaggatc
    11281 cagatcattt acaggcgata tccaaaattc tattccacta gaattcaaat gctgcctgag
    11341 gattggctga aaatgcttgg attgaagccc aggggagatc tgaaggaaaa gaagatcatt
    11401 atctatgaaa tgctccgtaa ggtgctgacc attatttacg gcatggagga attctctttt
    11461 gaagggtttg aaaaacttta catcggcgaa gagaagacaa aagatgatgt ttacgcagct
    11521 tacgaccggt atataaagga attaaaagaa agtgggcggc tgggcagtga aaacttctac
    11581 gcctattcca gggattctgt aaagaagttt gccggggata aaagactttc gttcaagagc
    11641 atcaatatac aattcctgaa aaagtatgag gcgcatatgc tcctgaaagg cgatgccctg
    11701 acaaccgtgg ccatgtacct gcgttgcctg aggtttcttt ataataatgc tatcaaagag
    11761 ggcattgtca ggcagcagga ttatcctttc ggcaggggca agtatgagat cccccagccc
    11821 cagaatatca agaaagcgtt gaagctgtcc gatatcggga agatattcca ctacaagcct
    11881 gaatcggagc aggaatccta ttaccgggat atctggatgt tttcttacct gtgcaacggc
    11941 atcaatctga aagacatctg cctgttgaag tattcagact tccagggaga tcacatttac
    12001 ttccggaggg ctaagacgat caataccaac cgtaattcaa aacccattga tatcatcgtg
    12061 accgatgaag tgcggcagat cattgaccgg tgggggaaca agcccgtcca gcctgacagt
    12121 ttcatctttc ctgtcttgac caacgatctt tcggcggaga agaagcacgc cgacatagcg
    12181 caggcagtcc agatgaccaa ccggtatatc cggcggattg ccaaaaagat cggcatcgaa
    12241 gcaaacgtca ccacctacac cgccaggcat tcgtttgcaa cggtcctgaa acggtcgggt
    12301 gtcaacgtga cttacatcag cgaagccctg ggccactccg acatcaagac caccgagaac
    12361 tacctggaca gcttcgaaga cgagcagaag aaagaaattg ccaagaaact gacagcgtgg
    12421 taaaatgcat tctgaaaata tttttaccaa aatgcatatc attttgaaaa ttaatttacc
    12481 aaaatgcata actttgactt gaaaattatt ctatatggtt caaaatggct taattgagga
    12541 gttgatcaaa ctcgacagcc tttccaaaga acaggcaggt aaatatcctt taaagcggtt
    12601 tcttttcccg aatttgaaat tcttgctgac taacaccagg cagatagttg gtattgcagg
    12661 tctcagggga accggcaaaa ccatattact gaggcaactt gccgtagatc tgggtcagtc
    12721 attttatctc agtgcagata cattgccgtt tgggacggac ctttttgatc tttctgccca
    12781 gcttgccggt acatttggta ttaaatacct gatggtcgat gaaatccatg ccctgaaaga
    12841 ttggcagggg caactgaaaa agatttacga tttccaggaa atcaggatca tttttacttc
    12901 atcctcttcg ctggaattgt ctgaagggag gtatgatcta tc
//
LOCUS       DAOPNH010000057        12860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5008_length_12860_cov_5.858493, whole genome shotgun sequence.
ACCESSION   DAOPNH010000057 DAOPNH010000000
VERSION     DAOPNH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_12860_cov_5.858493"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="PKZ74_04560"
     CDS             complement(<1..1360)
                     /locus_tag="PKZ74_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPM92062.1"
                     /translation="MSLSDKVRQKPWIGWAIFIATAVVVFFLGLLANSVMERRTEAQF
                     AFSTAVKYDNSEPRNAVWGKNFPREYQSYLQTSDTSFRSKYNGGAMIDMLEVDPRLVV
                     LWAGYGFAKDYNQGRGHYYAIEDIRNTLRTGGPAEGKPSPQPNTCWACKSTDVPRMMK
                     VTGPAEFYNGTWDTKGHEIVNAIGCADCHDAKTMNLQISRPALKEAFERKGIDLAKVS
                     HQEMRSLVCAQCHVEYFFDKREPKHNYLTFPWDNGYTAEDIERYYDSFMFTDWTHALS
                     KAPMLKAQHPDWEVYMTGIHAERGVSCADCHMPYKSEGGQKFTDHHLQSPLNNVEASC
                     AVCHREKTTTLIKNVYDRQDKIIENRDKLEELLVRAHVEAKKAWDLGASEEQMKPILM
                     DIRHAQWRWDYAAASHGASFHAPVEIGRVVSTGINIAQDGRIKLARLLASLGHNEEIP
                     YPD"
     assembly_gap    1407..1506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1507..2099)
                     /gene="nrfH"
                     /locus_tag="PKZ74_04565"
     CDS             complement(<1507..2099)
                     /gene="nrfH"
                     /locus_tag="PKZ74_04565"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPM92063.1"
                     /translation="MQGKIVNYFIPPPQWRLPVIIMLGIFVGLALFTFRTSRAWSYLS
                     DDPETCMNCHIMAPQFATWSHSSHRGKANCNDCHVPHNNFFNKYYFKAKDGLRHATIF
                     TLRAEPQVIFIKEAGVKVVKDNCVRCHEQQMACKHCHNKIIPEGDGIFCEREDEISML
                     NGEFTEYHADRLCWECHRETPHGRVNSISSVPNARVPV"
     gene            complement(2080..3726)
                     /locus_tag="PKZ74_04570"
     CDS             complement(2080..3726)
                     /locus_tag="PKZ74_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HPM92064.1"
                     /translation="MRSGPKDCHNGNRWLKFFKKWHRWPGVIMGFFFILWAISGVVMN
                     HRQLFSNFDITRNLVPKENRYVNWNNAAVRSGVMIGNDSLLLFGNTGIWLTDTRFAAF
                     KDFNAGFPEGIDNRKISSMLVSSKGNVYAGTLFGLFYLDFDKEKWVKIPVKFKDERIQ
                     WIIEKEGRIVFLTRSEMFESVDNPASFNPVGLKLPPPAGYDNKTGLFRTIWVIHSGEI
                     YGKIGRLVVDALGLVVILLAVTGSLHFAMPYALRTLKKRKKSLKLASATKKASATWHK
                     KAGIWIAVFILINTITGMFLRPPLLIAIANARVGKIPFSVMDTPNPWEDKLRAVIWDE
                     EIDGYILGTTEGLFFADNSLEREMVPLPGQPPLSVMGINVFQKTGSGQYLVGTFNGIY
                     DWYLKRDGVPPDGVPGTRDPISFYPDGVSTFRDGVSFNHLSGRPPQPSNSNSKPFGDE
                     MVSGYLKLENGNSVYFDYNKGAVSLGNEPVMPAMPLEIIEKSPMSWWNFALEVHTGRI
                     FKPLVGDFYILIVPLLGIFGTVLIISGIIVWIKLYARKNR"
     gene            complement(3730..4248)
                     /locus_tag="PKZ74_04575"
     CDS             complement(3730..4248)
                     /locus_tag="PKZ74_04575"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPM92065.1"
                     /translation="MEQLIITPRTKVYELLEAYPQLEDTLIEIAPVFKKLKNPVLRRT
                     IARVTTLQQAAQVGQVPVHEMVNTLRQKVGLDRLEGLEATASGNASKPSWLIEDKIVK
                     RLDARPVIEQGGHPLGEVLTGVGDLKPGEIYELVTPFLPAPLIERVVAQGFDHWSDKK
                     AEDHFINYFLKK"
     gene            complement(4322..5557)
                     /locus_tag="PKZ74_04580"
     CDS             complement(4322..5557)
                     /locus_tag="PKZ74_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPM92066.1"
                     /translation="MSELINNSEKRKELLKHMILQLHAGEAPDEARKQLVSLLTKIPY
                     GEVVEVEQELISEGTLSEAEVLKFCDIHSEALDGAIDLSGMKIIPPGHPVDTFKRENK
                     ELLKVVDQLESLYTRVSSVKSHEETAAFLIQLKTLFNSLMDVEKHYLRKENLVFPFME
                     SYGITGPPKVMWGKHNEIRELLKASIEVLNAPGQLSPEEMETVVQMVLKPASDGVADM
                     TLKEDQILLPMVMDKLTDLEWYEVYKQTLEIGFCLYDPDIEWRPENIAAEEVADAGND
                     LVHLPSGSFTKAELQAILNTLPVDMTFVDKNEKVKYFSQGKERIFHRSRAILNRDVKL
                     CHPPSSVHVVEQILEDFRTGRQDHAPFWIQMKGKFIYIEYFALRDENGEYLGTLEVSQ
                     DLTEKRALEGEQRILAYGK"
     gene            complement(5628..5957)
                     /locus_tag="PKZ74_04585"
     CDS             complement(5628..5957)
                     /locus_tag="PKZ74_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM92067.1"
                     /translation="METPLFSHSQVMNFNDKVEYSAEGIVSKRVIQKEKGNVTLFAFD
                     KGQKLSEHSAPFDAMVQVLEGQAEILINRVPYLLGAGQSIIMPANIPHAVNAVEKFKM
                     LLTMIKE"
     gene            complement(6082..6948)
                     /locus_tag="PKZ74_04590"
     CDS             complement(6082..6948)
                     /locus_tag="PKZ74_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM92068.1"
                     /translation="MKREIIKVDEEKCNGCGLCIPSCPEGALQIIDGKARLVSDLMCD
                     GLGACIGECPEGAIEIEEREAEPYNEVAVIKEIVKFGENTVVAHLKHLLDHNEMMYFE
                     TAVGYLEENSGSIGFDLEDVLDKVYAKSVGGDCAHGGCPGAKSFERDGVPRDEVPGDA
                     VPSQLRQWPVQMHLINPSAPHFRNAEVLLAADCVAFSVGNFHQKYLKGRSLAIACPKL
                     DQGKEVYVEKLKTMIDEANIKELNVMVMEVPCCSGLVHIANLAREAASRKIQIKKTII
                     GIKGEVLMESYI"
     gene            7263..7925
                     /locus_tag="PKZ74_04595"
     CDS             7263..7925
                     /locus_tag="PKZ74_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPM92069.1"
                     /translation="MYQALINSPIFTGLSEEELEKLFQRCHCFTKNYEVGDIAAFSGE
                     PVNFLIIVLEGSVKGEMVDFSGKIIKIDDIRAPYPLASAFIFGKQNKFPVNVMANERT
                     KLLYIPKYDFMKMLQADIRLMQNFLNVISGHTQILANKLKFLSFKTIKGKIAQYLLSL
                     AGPDKDLIELPLTQSDLAEQFGVTRPSLARALGEMADEGMISVDRKVVRILDRRRLLQ
                     IG"
     gene            7974..8648
                     /locus_tag="PKZ74_04600"
     CDS             7974..8648
                     /locus_tag="PKZ74_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92070.1"
                     /translation="MIWKGNTVVCLIIGVISHFLLISCSPYRQSKIAEYHERLVPPNC
                     IEISDNYFVDKSDIKNIDYLEFMFWNERIFGYNSQEYQSILPDSVKKHIPDSTYAGML
                     TRFYAGIMPETLELPLSNISDQQFRLYTAWRTDRVYEMMLSRFSILDYDTAQKAGHYF
                     TIEYYYNGIYSGSIPDTILAYIPIYSIPAQEEILVIKQNKTFNRKIKSPWTGYRNVCR
                     WERIGK"
     gene            8800..9198
                     /locus_tag="PKZ74_04605"
     CDS             8800..9198
                     /locus_tag="PKZ74_04605"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM92071.1"
                     /translation="MNEIIAYPEDFMFQLTIKEWRELITICDNLGAYKYSPSSPWAFT
                     EQGVAMLSGILRSPLAIKVNIAIMRAFVRMRELLENNKELKDKIEQLDNKYDGQFKVV
                     FEALKLLMEKENEPRNPVGFKIPGRDRNNL"
     gene            complement(9390..9707)
                     /locus_tag="PKZ74_04610"
     CDS             complement(9390..9707)
                     /locus_tag="PKZ74_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPM92072.1"
                     /translation="MKTSYVYIITNKNRTVFYTGLTANLTNRMIRHKAGKGSKFCAKY
                     NVNTLVWYETHIHIMNAIRREKQIKHWNRQWKIDMIRARHPEMKDLLAKQGGSGAGGS
                     SPG"
     gene            complement(10033..>11241)
                     /gene="hydF"
                     /locus_tag="PKZ74_04615"
     CDS             complement(10033..>11241)
                     /gene="hydF"
                     /locus_tag="PKZ74_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPM92073.1"
                     /translation="ELKPHIGIFGRRNNGKSSLINALAGQDVAIVSAQPGTTTDPVRK
                     SVEIFGIGPAILVDTAGIDDSGDLGELRIRKTLQVIKAIDLAIIVITDNSISQFENEL
                     IRQFGNYEVPYIFVHNKSDLVELSEETRKIVQKEFTTDITDFSSLNPTNKEQLIEMII
                     RHMPETAYTSQSLLGGLLNRGDIVMLITPIDNEAPEGRMILPQVQAIRDVLDNDCINV
                     VLKETEVEHFLKTTGIKPRLAVTDSQMFGRIDKVIPKDIPLTGFSVLLARHKGPFGKY
                     MEGTPKIDQLNDGDRVLILESCTHQVNCDDIGRYKIPTWLSEYTGKKLEFDIVAGLDS
                     FESDFRKYALIVQCGGCMITRKQVHSRLKEAIEAGIPITNYGMAIAWTKGIYQRAIEP
                     FAAVSPLARG"
     assembly_gap    11242..11341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11342..11830)
                     /locus_tag="PKZ74_04620"
     CDS             complement(<11342..11830)
                     /locus_tag="PKZ74_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPM92074.1"
                     /translation="MKTLVDSILTKGILTKDDLVYLLSTKEEKEKHSLFRKANQVKKE
                     HCGNVVYLRGLIELSNICRKDCNYCGIRKSNEKVVRYTLPEDEVIDRALFALRRDFGS
                     VVIQSGERTDRVFIDMLTRVIKAIRERSEGTLGITLSCGEQSLETYQQWFDAGAHRYL
                     LRI"
     gene            complement(11953..>12860)
                     /gene="hydG"
                     /locus_tag="PKZ74_04625"
     CDS             complement(11953..>12860)
                     /gene="hydG"
                     /locus_tag="PKZ74_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPM92075.1"
                     /translation="VAVLRGAALERDVGEGEAGVGTYQIFQETYHPETYKKYHTGGFK
                     RDYENRLTSLDRAQEAGLDDVGIGVLFGLYEWRYEVLALVRHANHLEAVYNVGPHTIS
                     FPRLQFASEYEISGDWLVSDDDFTKLVAILRLAVPYTGLILTAREPEHIRREVLQFGV
                     SQIDAGTKLEIGAYAEEELPTAQNPDKEQFLINDNRSLREVIDELLDEGFIPSFCTAC
                     YRKGRTGEHFMEFSVPGFIKRYCTPNALLTLAEYLEDYAPAPTAEKGWELILKNLEEE
                     VDPKFTAEVKKRLERIKAGERDLYF"
BASE COUNT         2978 a         3045 c         3128 g         3509 t
ORIGIN      
        1 tatcggggta agggatctcc tcattatggc caagggaagc gaggaggcgg gccagtttga
       61 tccggccgtc ctgggcgata ttgatgccgg tggaaacgac ccggccaatt tccacgggag
      121 catggaagga tgcgccatgg ctggcggcag cgtagtccca gcgccactgg gcgtggcgga
      181 tatccatcag gatcggtttc atctgttctt ccgatgcgcc caggtcccat gcttttttgg
      241 cctccacatg tgccctgacg agcagttctt cgagtttatc gcggttctcg ataattttat
      301 cctggcggtc atagacgttt ttgatcaggg ttgtggtttt ctcgcggtga catacggcgc
      361 aggatgcttc cacgttgttc agcgggctct ggaggtggtg gtcggtaaat ttctgcccgc
      421 cttcgctttt atagggcata tggcaatcgg cgcaggagac gccccgttcg gcgtgaatgc
      481 cggtcatata cacttcccag tcggggtgct gggctttcag catcggcgct ttgctgaggg
      541 catgggtcca gtcggtaaac ataaacgaat cataatagcg ttcgatatct tccgcagtat
      601 atccgttatc ccacgggaag gtcaggtagt tgtgtttcgg ttcgcgttta tcgaaaaagt
      661 attcgacgtg gcactgggca cagaccagtg agcgcatctc ctggtgggag actttagcaa
      721 ggtcgatgcc ttttctttca aaggcttctt tcaatgcggg ccggctgatc tgcaggttca
      781 tggttttcgc gtcgtggcag tcggcacaac cgatcgcatt gacaatctca tggccttttg
      841 tatcccaggt cccgttgtaa aattcagccg ggccggtaac tttcatcatc ctggggacat
      901 cggtgctttt gcaggcccag catgtattgg gctgcgggct gggtttccct tcggcagggc
      961 cgccggtccg aagtgtattc cggatatcct cgatggcata gtaatgcccc cgtccctggt
     1021 tataatcttt ggcaaagcca tagccagccc acaggacgac gaggcgcgga tcgacctcca
     1081 gcatatcgat catggcaccg ccgttgtatt tgcttcgaaa cgacgtgtcg gaagtttgga
     1141 ggtatgactg gtactcccgg gggaaatttt taccccagac ggcatttcgc ggttcggagt
     1201 tatcgtactt tacggcagta ctaaaagcaa actgcgcttc ggtccgccgt tccataacgg
     1261 aattggcgag aagtcccagg aagaaaacaa ccactgcggt ggcaatgaag atcgcccacc
     1321 cgatccaggg cttttgacgg actttgtctg ataatgacat aatgcttaat gtttaatgag
     1381 taatgcttaa tgctttaatg cttaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnacgg ggacgcgggc gtttgggacg ctggagatgc tgtttacgcg gccgtggggt
     1561 gtttcgcggt ggcattccca gcaaagcctg tcggcgtgat actcggtgaa ttccccgttc
     1621 agcatgctga tttcatcttc acgttcgcaa aagatcccat caccttcagg tattatttta
     1681 ttatgacaat gtttacaggc catttgctgc tcatggcatc ttacacaatt atctttaaca
     1741 accttaaccc cggcttcttt gataaagatg acctggggtt cggcccggag ggtaaagatg
     1801 gtggcatgtc gcaatccatc tttggcttta aaatagtatt tattaaagaa gttattatgc
     1861 gggacatggc agtcgttgca gtttgctttg ccgcggtgtg agctgtggct ccaggtggcg
     1921 aactgggggg ccatgatatg gcagttcatg caggtttcgg gatcatcgga gaggtacgac
     1981 caggccctgg atgttctgaa agtaaagagg gccaatccga caaaaatacc cagcatgatg
     2041 ataaccggca accgccattg aggcggcggg atgaaatagt taacgatttt tccttgcata
     2101 gagttttatc cagacaatta ttccgctaat tattaaaaca gtaccaaaaa tacccaataa
     2161 aggtacgatt aatatataaa agtcgcccac gagcggttta aagatccttc cggtatggac
     2221 ctccagggca aaattccacc acgacatcgg gcttttttct ataatctcaa gaggcatagc
     2281 cggcataaca ggttcattgc caaggctgac cgctccttta ttataatcaa agtaaaccga
     2341 atttccattt tccagtttca aatacccgct caccatttcg tcaccgaagg gtttgctatt
     2401 tgagttggaa ggttgtgggg ggcgaccgga gaggtggtta aatgagactc catctcgaaa
     2461 tgttgagact ccatctgggt agaatgagat ggggtctcga gtccccggga cgccatccgg
     2521 tgggacgcca tcccttttca ggtaccaatc ataaattcca ttgaatgtgc cgacaagata
     2581 ctgaccggaa cctgttttct gaaaaacgtt tatccccatc accgaaagcg gcggctggcc
     2641 cggaagggga accatttccc tttcgagact attatcagca aaaaacaatc cttcggtagt
     2701 acctaaaata tatccgtcta tttcttcatc ccaaatcacc gcccgaagtt tatcttccca
     2761 gggattgggc gtatccatga ccgaaaaagg aatcttcccg acacgggcat tggcaatggc
     2821 aatcaataac ggcggacgaa gaaacatccc tgttatcgta ttaatcaaaa taaacacagc
     2881 aatccagatc ccggcttttt tgtgccaggt tgccgaagcc tttttagtgg cagaggccag
     2941 cttcagggat ttttttcttt ttttgagagt ccttagggca tagggcattg caaaatgcaa
     3001 agaacctgta actgccagca gaataacgac caggccgagg gcatccacca ccaaccgtcc
     3061 gatcttacca taaatttccc cgctatggat cacccagatc gtcctgaaaa ggccggtctt
     3121 attgtcatag ccggcagggg gcggcagttt cagtccgact ggattaaaag acgcgggatt
     3181 atcaaccgat tcaaacattt cagaccgcgt caggaagacg atccttccct ctttttcaat
     3241 aatccattgt atccgttcat ccttaaattt aaccggaatc ttaacccatt tttccttgtc
     3301 aaaatctaaa taaaataaac cgaatagcgt acctgcataa acattccctt ttgaggagac
     3361 cagcatcgac gatatcttcc ggttgtcgat accctcagga aatccggcat taaaatcttt
     3421 aaaagcggca aaccgggtat cagtcagcca gatgccggta ttcccaaaaa gaagcaggct
     3481 gtcgtttccg atcatgacac ccgatcgtac cgcggcattg ttccagttga cataccggtt
     3541 ctccttcggg acgaggttcc tggtgatatc aaaattggag aataactgcc tgtggttcat
     3601 gacgacaccg gagatggccc agaggatgaa aaagaacccc atgatcactc cgggccagcg
     3661 gtgccacttt ttaaagaatt tcagccatcg gttaccgtta tgacagtcct tcggaccgga
     3721 tctcatggct tatttcttaa ggaaatagtt tataaaatga tcttcagctt tcttatccga
     3781 ccagtgatcg aagccctggg ccacaacccg ttcgatcaac ggggccggca ggaaaggggt
     3841 aacgagttca tagatctcgc cgggttttaa atcacccacc ccggtaagca cttcgcccag
     3901 cgggtggccg ccctgttcta tgaccggcct ggcatccagg cgcttcacta ttttatcttc
     3961 tattaaccag gatggtttcg aggcatttcc ggaggctgtc gcttccagtc cttccaggcg
     4021 gtcgaggcca actttctgcc tgagggtgtt caccatttca tgcacaggca cctgcccaac
     4081 ctgggctgcc tgctgcaaag tggttaccct ggctatcgtc cgccgcaaaa cggggttttt
     4141 cagcttttta aagaccgggg ctatttcgat cagcgtatct tcgagttgag gataggcctc
     4201 caggagttcg taaacttttg tgcgtggggt tatgattagt tgttccattg ttcgattgtt
     4261 cgattgttat ttggttaggg atccaaattt ccaatttcaa atttccaatt acgacataag
     4321 gctactttcc atacgccagt atcctttgtt ctccttcaag tgcccgcttt tcggtcaggt
     4381 cctgcgatac ttccagggtg ccgaggtatt cgccgttttc gtcgcgcaag gcaaagtact
     4441 cgatgtagat gaactttcct ttcatctgga tccagaaggg ggcgtggtcc tgccggccgg
     4501 tcctgaaatc ttccaggatt tgctctacca catgtacgct ggatggcggg tggcagagct
     4561 tgacatcacg gtttaggatg gcgcggctgc ggtggaatat gcgctctttt ccctggctga
     4621 aatatttcac tttttcgttt ttatcgacga aagtcatatc gaccggcaag gtattgagaa
     4681 tagcctgcag ttctgctttg gtaaaactgc cggaaggaag gtggaccagg tcgttaccgg
     4741 catctgcaac ctcttcagcg gcaatatttt caggtcgcca ttctatgtcc ggatcataaa
     4801 gacagaagcc gatttccagg gtttgtttgt agacttcata ccattccagg tcagtcagtt
     4861 tatccatgac catcggtagg agaatctggt cttctttcag ggtcatatct gcaactccgt
     4921 cggaagcggg tttgagaacc atctgcacta ctgtttccat ctcttccgga gaaagttgtc
     4981 ccggggcgtt caatacttcg attgaagcct tcagcaattc cctgatttcg ttatgtttac
     5041 cccacattac cttaggcgga ccggtgattc cataactttc cataaagggg aataccaggt
     5101 tttccttacg gagataatgc ttttcgacgt ccatcaggct gttgaagagc gttttaagct
     5161 ggatgagaaa agcggcggtc tcttcgtgac tcttaacgga ggagacccgg gtgtaaagcg
     5221 attcgagctg atcgaccacc ttcaggagct ccttgttttc ccttttaaag gtatcgaccg
     5281 gatggccggg agggattatt ttcatacccg aaaggtcgat ggcgccatcg agggcttcgg
     5341 agtggatatc gcagaatttg agtacttcgg cttcgctgag ggtgccttcg gagatcagtt
     5401 cctgctcgac ctccacgact tcgccgtaag ggattttcgt caggaggctg accagttgtt
     5461 tgcgcgcttc gtcgggtgct tcgccggcgt ggagctgcag gatcatgtgt ttgagcagct
     5521 ctttgcgctt ttcggagttg ttgataagtt cgctcatatc taattgcccc tacccctgta
     5581 tcccctcccc caattggggg aggggaccaa ggggaggggg cttggtttca ctctttgatc
     5641 attgtcaata gcatcttgaa cttttccaca gcatttacag catgaggaat attggcgggc
     5701 atgatgatag attgtccggc gccaagcagg taaggaacgc ggttgatcag gatctcggcc
     5761 tggccttcca gtacctgcac catagcgtcg aagggtgcgg aatgttcgct gagtttttgt
     5821 cctttgtcaa aggcaaatag ggtcacatta cccttttctt tctggatgac gcgtttggag
     5881 acaattcctt cggcggaata ttcgacttta tcattgaagt tcattacctg ggagtgtgag
     5941 aataagggag tttccatgat attaatttta aattttaagt tttaaatttt gaattcaggt
     6001 atttttttag tcctaagtgt ttagttctaa gttcgggaat atttttactc tgtgttaact
     6061 ctgtgtactc tgtggtgaat atcaaatata gctttccata agcacctctc ctttgattcc
     6121 gatgatggtc ttttttatct gaattttccg gctggcggct tcgcgggcca ggttggcgat
     6181 atgcaccagg ccgctgcagc agggcacctc catgaccatc acgttgagtt ccttaatatt
     6241 tgcttcatcg atcattgttt tcagtttttc gacatagacc tcttttccct ggtcgagctt
     6301 ggggcaagcg atagcgaggc tgcgtccttt caggtatttc tggtggaaat tgccgacgga
     6361 aaaagcgaca caatcggctg ccagcagcac ctccgcattt cggaaatgag gtgcggaagg
     6421 gttgatgagg tgcatctgga cgggccattg gcggagctgt gaagggacgg cgtccccagg
     6481 gacctcgtcc cgcgggacgc catccctttc aaaactcttt gctcccgggc atccgccatg
     6541 cgcgcaatca ccaccgacac tcttcgcata gaccttatcc agcacgtctt ccaggtcaaa
     6601 tccgatgctt ccggaattct cctccagata cccgactgcc gtttcaaaat acatcatttc
     6661 attgtgatcg agcaaatgct tcaggtgcgc tacaacagtg ttttccccga acttcacgat
     6721 ctctttgatc acagccacct cgttgtaagg ctcggcttca cgttcctcta tttcgatagc
     6781 gccttccggg cattcgccga tgcaggcacc gagtccgtcg cacatcaggt cactgaccag
     6841 ccgcgctttg ccgtcgatga tctgtaaggc cccttcaggg caggagggga tgcaaaggcc
     6901 gcatccattg catttttctt cgtcgacttt aatgatttct cgtttcatat tatggtcatt
     6961 agctcgctgc gctcgcgtca ttaagtggtc atttactcac ttcgttcgtg tcattggctc
     7021 gctgcgctcg cgttagtgtt ttttactctg tgttactatg tgcaactctg tgtaatttgt
     7081 ggtgagtctt aaaacttccg gcaacaaaaa tagccttcaa ctttagaaca ggatgtaaca
     7141 aatgttacaa caattcgaga tggcatctca aatttcacta tttttgtttt ctattgttag
     7201 aatgataagg ttttccggaa ggagctccat ataaaagaga tgccatctca ataagcatag
     7261 aaatgtatca ggcactaata aatagcccga tatttaccgg actttctgaa gaggaactgg
     7321 agaagctctt tcaaaggtgc cactgcttta ctaaaaacta tgaagtgggc gacatagccg
     7381 ctttcagcgg cgaaccggtg aacttcctga tcatcgttct cgaagggagt gtgaaaggcg
     7441 aaatggtcga tttctcggga aagatcatta agatcgacga tatccgggcg ccatacccgc
     7501 tcgcttcggc ctttattttc gggaaacaga acaaattccc ggtgaatgtg atggcgaacg
     7561 aaaggacaaa attgctttac ataccgaaat acgatttcat gaagatgctg caggccgata
     7621 tccgcctgat gcagaacttt ttgaatgtga tttcgggaca tacgcagatc ctggcaaata
     7681 agcttaaatt cttatctttc aaaacgataa aaggaaaaat cgcccagtat ctcctctcac
     7741 ttgccggacc cgacaaagac ctgatcgaac ttccccttac ccagagcgac ctggccgagc
     7801 aattcggcgt gacgcggcct tcactggccc gggcactcgg ggagatggcg gatgaaggga
     7861 tgatctccgt ggacagaaaa gtggtgagga tattggacag gaggaggctg ctgcagattg
     7921 ggtgatttgg ggattagagg atttggggat atttgatttt tgatttagga tttatgattt
     7981 ggaagggaaa tacagttgtc tgtttaatta ttggtgttat aagtcatttt ttattgattt
     8041 cgtgctctcc ttacaggcaa agtaaaattg ctgagtatca tgaacggctg gtacctccta
     8101 attgtattga gatttcagat aattattttg tagataaatc agatattaaa aacattgatt
     8161 acctggaatt tatgttctgg aatgagcgta ttttcggata taattctcag gaatatcaga
     8221 gcattctgcc tgattccgtt aagaagcata ttccggattc gacttatgca ggaatgttaa
     8281 caagatttta tgcaggcata atgccggaaa ctcttgaact gccgctaagc aatatcagtg
     8341 atcagcaatt caggttatat acagcgtgga gaaccgaccg ggtttatgaa atgatgcttt
     8401 cccgcttttc aatcctggat tatgatacgg ctcaaaaagc cggacattat tttaccatag
     8461 aatattatta taacggcatt tattcgggtt ccatacctga tacgatttta gcttatattc
     8521 caatttacag tattccagct caggaagaaa tattggtaat aaaacaaaat aagaccttca
     8581 atagaaaaat aaaatctccc tggacaggtt atcgcaatgt ttgtcgatgg gaaagaattg
     8641 gaaaataggc taccggagga attaggataa gttgacgaat tcatgcatca tcactcgaaa
     8701 gaggggagat caagatcaac cacttacaat taattttcat cattacaggg tgttttttga
     8761 taaaaaccgg ataaatagat aatattaata acactttatt tgaatgaaat tatcgcttat
     8821 cctgaggatt ttatgtttca gctaaccatc aaagagtgga gggagcttat cacaatttgt
     8881 gataaccttg gcgcttacaa atattctcca tcctctccat gggcttttac cgaacagggt
     8941 gtggccatgc tttcaggtat attaagaagc cctttggcga ttaaagttaa tatagccatt
     9001 atgcgtgcat ttgtcaggat gcgtgaactc ctggaaaaca ataaggaatt aaaagataag
     9061 atcgaacagc ttgataataa atatgatgga caattcaaag tggtgtttga ggctttaaaa
     9121 ttgcttatgg aaaaagaaaa cgaacccaga aatccggttg gatttaaaat acccggtcgg
     9181 gatagaaata atttgtaagt aattgacttt gtgatatttg cttagccggc agtttccgac
     9241 ttgcgtggga atgaccgcga atcaccgaga acagacgggg cggcgcggct gccgagggaa
     9301 ttgcgaattg ctatgccgtt tgcagccgcg ccgccctggt tcctctctcc acgcgccaac
     9361 cccgtcattc ccaagccagc gtgaattcct tagccaggcg atgacccgcc agccccggag
     9421 cctccttgct tcgcgagcag atctttcatc tccggatgcc tggcccggat catatcgatc
     9481 ttccactgcc ggttccagtg cttgatctgc ttttcgcgcc tgatggcatt catgatatgg
     9541 atatgggttt cgtaccagac cagagtattt acgttgtatt ttgcgcaaaa cttcgatccc
     9601 tttccggcct tgtggcggat cattcggtta gtgagatttg cggtcagccc ggtataaaat
     9661 accgtccggt ttttattggt aataatgtat acgtatgatg ttttcatacc tattaatccg
     9721 gaattgtgct aaaaaatacc tggtagggtt gaaataattt tgtatgtagt tggcattgag
     9781 tgtgatgcgg aggctggtag tcccctcttt cgaggggatg acgggttccc tctttcgagg
     9841 ggatgacggg ttccctcttt cgaggggatg acgggttccc tgaatttagg tggttccccg
     9901 cttacgcggg gatgacgttg taccgctgga aatagaggaa acagagcggc ggcgatgcaa
     9961 agttgattgc tattataaaa gtcatcagca tcgccgccgc tatttttttc tcttttatcc
    10021 ggtgcagtag cgtcatcccc gcgccagcgg ggacacggcg gcaaacggct caatcgctcg
    10081 ctgatagatc cctttcgtcc acgcaatcgc catcccgtag ttcgtgatgg gaattccggc
    10141 ctcaatcgct tctttcagcc ggctgtggac ctgctttctt gtgatcatgc acccgccgca
    10201 ctggacgatc agggcatatt tcctgaaatc gctctcgaag ctgtcgagcc cggcgacgat
    10261 gtcgaattcg agcttctttc cggtatattc gctcagccag gtgggtattt tgtaccggcc
    10321 gatgtcgtcg caattcacct ggtgcgtgca gctctccagg atcagcaccc ggtcgccgtc
    10381 gttgagctgg tcgatctttg gcgtcccttc catgtatttg ccgaacggac ctttgtgacg
    10441 cgccagcagc acgctgaaac cggtcagcgg gatatctttc gggatcacct tgtcgatccg
    10501 gccgaacatc tggctgtcgg tcacggccag ccggggcttt atgccggtgg ttttcagaaa
    10561 atgttcgacc tcggtctctt ttaaaacaac gttaatgcaa tcattgtcaa gcacatcacg
    10621 gatagcctgc acctgcggca agatcatcct tccctcgggc gcttcgttgt cgatcggggt
    10681 gatgagcatc acaatatccc cgcggttcag taatccgccc agcagcgact ggctggtata
    10741 tgccgtctcg ggcatgtggc ggattatcat ttcgatcaat tgctccttgt tggttggatt
    10801 taagctggag aaatctgtga tatccgtggt gaattctttc tgtacgatct tcctggtttc
    10861 ttccgataat tcaaccagat ccgatttatt atgaacgaaa atatagggaa cttcataatt
    10921 tccaaattgc cggattaact cattttcaaa ttgactgatt gaattatccg taataacgat
    10981 gatggccagg tcgatggcct tgatcacctg gagggttttg cggatgcgca gctcgcccag
    11041 gtcgccgcta tcatctatcc cagcagtatc gaccaggata gccgggccaa tgccgaagat
    11101 ctcgaccgat ttgcgcaccg ggtcggtggt ggtgccgggc tgcgcggaga cgatggctac
    11161 gtcctgcccg gccagggcgt tgatcaggga gcttttcccg ttgttgcgcc tgccgaatat
    11221 gccgatatgc ggcttgagtt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 naattctcag caaatagcgg tgagcgcccg catcgaacca ttgctggtag gtttccaggc
    11401 tttgttcacc gcaactcagc gttatcccga gcgtgccttc cgatcgttcc ctgatggctt
    11461 tgatcacccg ggtcagcata tcgatgaaca cacggtccgt tctttccccg gattggatga
    11521 ccacggagcc gaagtccctg cgcaaagcaa aaagggccct gtcgataacc tcatcctcag
    11581 gcagggtgta cctgacaacc ttttcattgc ttttcctgat cccgcagtaa ttgcagtctt
    11641 ttctgcagat gttggaaagc tcgatcaatc ccctgaggta caccacattg ccgcaatgtt
    11701 cttttttaac ctggtttgcc ttgcggaaaa gggagtgctt ctctttttct tccttcgtcg
    11761 ataacaggta gaccaggtca tcttttgtta aaatgccttt ggttaaaata ctgtctacca
    11821 atgttttcat ttcttctttt tgtttatccc ctacggggaa aattgatatc tgggattaat
    11881 ccggtctacc ataatatatc cgctacgcgg aattatatcg accatcgaaa atcgacaatc
    11941 gacaatcgac aatcaaaagt acaaatcccg ctctcccgcc ttgatccgtt caagcctctt
    12001 tttcacttcc gctgtaaatt tcggatccac ttcttcctca aggtttttca atatcagttc
    12061 ccagcctttc tcagccgtcg gcgccggggc ataatcttcc aggtattcgg ccagggtgag
    12121 caaggcattg ggtgtacaat atctttttat aaagccgggg accgaaaact ccataaagtg
    12181 ctcgcccgtc ctgcctttcc ggtaacaggc agtacaaaat gacgggataa agccttcgtc
    12241 caggagttcg tcgatcactt cgcggagaga acggttatcg ttgatcagaa attgctcttt
    12301 atctggattt tgtgctgtcg ggagctcttc ttccgcataa gcgccgatct cgagcttagt
    12361 accggcatcg atctgcgaaa cgccgaactg caggacttcg cggcggatat gttccggttc
    12421 gcgtgcggtc aggatcagcc cggtataagg aacggccagg cggagaatgg ctaccagctt
    12481 cgtgaaatca tcatcgctca cgagccagtc gcccgatatt tcatattccg atgcaaactg
    12541 caatcgcgga aaggagatgg tgtgcggccc gacgttgtaa acggcttcga ggtggttggc
    12601 gtggcgaacc aacgccaaca cctcgtatcg ccattcatac aaaccaaaca gaacaccaat
    12661 gccgacatca tccagaccgg cttcctgtgc gcggtcgagc gatgtcaggc gattttcgta
    12721 atctctttta aatcctccgg tatggtattt tttataggtt tccggatgat atgtttcctg
    12781 gaagatctgg tatgtaccga ccccggcctc accttctccg acgtcacgct cgagcgcggc
    12841 gccacgcagg accgcgacct
//
LOCUS       DAOPNH010000058        12796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5055_length_12796_cov_6.159721, whole genome shotgun sequence.
ACCESSION   DAOPNH010000058 DAOPNH010000000
VERSION     DAOPNH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_12796_cov_6.159721"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKZ74_04630"
     CDS             complement(<1..666)
                     /locus_tag="PKZ74_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM92076.1"
                     /translation="MKRIMNIIQKQYRTKAIICAAVVTLMSFIVLPVSSQIIAGQKNI
                     CLSGQLTSTNTGAPIADHEIYITSDSLTNGGFGYYAVTRTDVNGFYWDTLTTTTNDGI
                     IKIYVYDFDNNRIELDRYYRFIWENQYLMFADFAIFDPEANPDLQANFIPTPDTLDDN
                     PMKIIFRDQSIGSSIKSWYWDFGDGKSSTVQDPDHIYDASGIYMVSLTITTMPPEYGV
                     SESS"
     gene            complement(673..2001)
                     /locus_tag="PKZ74_04635"
     CDS             complement(673..2001)
                     /locus_tag="PKZ74_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPM92077.1"
                     /translation="MAEEFKHIDEIIRQKFDNFEPEPPLHVWENIKSSLPKDPAPPSS
                     PGILLPIIVTISLIIFIAGLFNQYYPDRPDTTAGESSSPYSTVQTAGVAPAGTTSNAN
                     LTLPEPAFQSPEIIPSPEEKKVKAEAKKQVPDIPVRAPFGENTKFSRKSKETRTVETT
                     PKLRKEPVAGYRPGIIRAVTLGTITYADAMRYDLTPREIRKLSVIAEKQRNVKPTWSL
                     GAYFNPEMTSYSDESLENSIGFNLAILPTISFSHFYVQSGFNARFTHDKGDYAIDFNR
                     YLGSYEDVYEVTFDSTENGVIPIYHTQTVDVYDTVDHYSISETKATYTYLEVPVLFGY
                     RHSFGRFGIRAQAGPAASFLILKDIPAASVPEENARIVNVNSLIPVRSTINWQLVVGA
                     GFDYELADRVIFSLEPTFRYALDPDFTMPENRNGRTHSFGIRAGLNYRFK"
     gene            complement(2010..2540)
                     /locus_tag="PKZ74_04640"
     CDS             complement(2010..2540)
                     /locus_tag="PKZ74_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM92078.1"
                     /translation="MYSDKELIEHCLANDPRAQEFLYKRFSRRMYGVCLRFARNTLEA
                     DDILQEGFIKVFAHLKDFRCDGSLEGWVRRVIVNTAINYYNSRLHELNETPIDKAVIM
                     NSTTEDILDKISAGDLLHLIQGLPEGYRMVFNLYVIEGYNHQEIAEMLKISENTSKSQ
                     LSRARTALQERLAQRK"
     gene            complement(2802..6233)
                     /locus_tag="PKZ74_04645"
     CDS             complement(2802..6233)
                     /locus_tag="PKZ74_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM92079.1"
                     /translation="MPVNSQPEKSQKVIRQFGLTNLVLKNKNSIYLLILILFGFGFYS
                     YTSMPKELFPDIVIPTVLVQTAYPGNPPLDMENLVTRPIEKELEVITGVKEIASTSSQ
                     DISSIVVEFNTGVDLKQAVLDVKDAVDKAKSELPDDLPADPLVQDIDFSEFPIIYINL
                     SGDYSLNELKKYAEYLEDEIETYSEISKVDIKGLNEREFSINVDPFKMEMLEISFGDI
                     ESAVGFENISMAAGQFREGDYRRSIRILGEFETAAQMGDIIVKSEKGNIVYLKDIAVV
                     TDGFAEPKDFSRLNRQPVVTLHVIKKGGENLLSATDKIFDLLDRSKEDGNLPEGLNIT
                     ITNDQSDLIRKQLDNLENSMIMGVILVVFVLFLFLGTRNALFVGFAIPLSMFISFLIL
                     NLMGYKLNMILLFSLILALGMLVDNAIVVMENIYRYFDRGYSRMEASRLATGEIAIAI
                     ISSTATTLAAFFPLLFWDSMIGEFMKLLPLTLIIVLTASLFVALTVTPVLTSSFLKQG
                     DQLPKPKIKRSLIIVAILLLLAGIFYAGGMNTPGSLFVLFAIIGIANLLFLNRMARWF
                     MNVFLPWLEDAYLNTMNFVMKARRPYWVIAATFFLLIFTIVLMGIRQPKVIFFPSPDP
                     SYINIMATLPVGTDITATNKFMGTFEEDVYSILTPYSRIVKSVLTTVGNGAKSENEGF
                     DISETPHRGMVTVTFVDFEFREGVNTNDILKAFSDNLIGKYPGVMVAVDKPSSGPPVG
                     KAINIEVTGEDFDKLVILVDDLLNKIDNAGIDGIEGLKTDLNLDKPEAIVHIDRDRAR
                     RYGLSTAQIAGTIRTALFGKEISDFKEGEEEYPIQLRLKEEFRYNVASLMNQKITFRD
                     QASGKIQQVPITAVADISYGSTYEAIKRKDLDRVITLSSNVLKGFNATDINNQLKEFL
                     SGETLPSGYTYKFTGEQQEQDESMAFLGSAMLIALALILLIMVSQFNSVVKPVIIMIS
                     VLFSTIGVFGGLATFRMDFVVIMTGIGLVSLAGIVVNNAIVLIDYTDLLKLRKRTELG
                     MEENALLPKDIALDCVIQAGRTRLRPVLLTAVTTLLGLIPLASGLNIYFLSLFEHFDP
                     KIYFGGDNVAFWGPISWTIIFGLTFSTFLTLIVVPAMYHVLYLGKIRIHEMRKRK"
     gene            complement(6316..7956)
                     /gene="lnt"
                     /locus_tag="PKZ74_04650"
     CDS             complement(6316..7956)
                     /gene="lnt"
                     /locus_tag="PKZ74_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPM92080.1"
                     /translation="MKKIHLISISIAGGLLLSLAWPERGFSPLLFIGFIPFLFIEDYI
                     FDHQDKFRKRSVFLYTYPGFLLWNILTTWWIWNSTDFGAIMAWVLNALFMAAIFWIFH
                     FTRRAMPGKQGQIPLIFFWIAFEYFHHNWDGTWPWLSLGNGFAGWHKWIQWYEYTGIF
                     GGTLWILAVNILAYDIFKGKGNREKGNGNWTMDTGQWKMNWVKGSALVLLIVVPVLLS
                     YRLYNKYEEKPWPVDVIVVQPDIDPYNEQYTLESTELLKANLDLAQKMMDNRVDFIAS
                     PESALQENIWENRLHDARGLASIREYLKGYPSAAYIIGASTFYRFGDDEKKSATARKF
                     RSAEGYYDAFNTAFYIDTNKRYQLHHKSKLTPGVEKMPLAVLLKPLESLALDLGGTVG
                     SLGTDKERTVFLRKSDSLKIAPVICYESVFGEYVTQYIRKGANLIFVITNDGWWGNTP
                     GHRQHLTFSKLRAIETRRSIARSANTGISAFIGQRGDIYQRTNYWEPAVIRQEINANN
                     FTTFYVRYGDYIARLSAFIAVFVLLISISTRLRKKVEA"
     gene            complement(8012..9214)
                     /gene="hflX"
                     /locus_tag="PKZ74_04655"
     CDS             complement(8012..9214)
                     /gene="hflX"
                     /locus_tag="PKZ74_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPM92081.1"
                     /translation="MPKGIIIKPVVERAVLIGLVKNRFDADRINDYLNELAFLVDTAG
                     GVAVKRFIQRIDLPDQRTYIGQGKLNEIKEFVKNEDINIAIFDDELTPSQSRNVEKEL
                     GIKVLDRNSLILDIFARRARTAHARTQVELAQYEYLLPRLTRMWTHLERQQGGIGLRG
                     PGEREIETDRRIVRDRIALLKEKLKEIDKIKTTQRKGRKELVRVALVGYTNVGKSTVM
                     NLLSKSDVFAENKLFATLDTTVRKVVIGNLPFLLTDTVGFIRKLPHSLVESFKSTLDE
                     VREADLLVHIADISHPHFEEQIAVVNQTLSEIGASNKPVIMLFNKIDAYTFVEKDPFD
                     LTPAGKENLSLDDLKKTWMSNSHYPSIFLSATQKVNLEEFKNMLYGEVRKIHTKIYPF
                     NNFLFEEF"
     gene            complement(9276..11078)
                     /locus_tag="PKZ74_04660"
     CDS             complement(9276..11078)
                     /locus_tag="PKZ74_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPM92082.1"
                     /translation="MNKIASLSKDQLKKKLWEANPEIRALLKKSSDVADARENLFEYL
                     NGLERHYFNIHSEKEYKDFHIIEKTNAKECIRVLKNVIRTENEKIADFSALKVLRDLA
                     LNSEPEIPVSESFLAEFIFLFHGVHARSGITDDSFKLPSDNKKASQLRSKKLDEYARR
                     MDDHLRHYRHGLDPAVIHDRKAASKAILEYYQGTEKDWQDYKWQLKHIITTRKTLEKL
                     VMLDEDERLGLEYAERYRIPFQITPYYLSLFNRKGRTKDDRAIRAQVLPSANYCINVD
                     KNRKSGFDMDFMGERSTSPAEAITRRYPHIVILKPVDICPQLCVYCQRNWEVKPMRES
                     KATRNKTAAAIDWIRNNENISEVLVTGGDPLILGDDYIETILQSLSEIGHVERIRIGT
                     RVLVTLPMRIGDSFIRILRKFHRPGKREICIVTHFEHPMEITPEAVSAIGRIRRAGIS
                     IYNQQVFTYYNSSRFASCALRKQLKLAGVDPYYTFNTKGKEETSDFRVPIARISQERK
                     EEARFLPGLARTDEPVFNVPKLGKSHLRAWQDHELIMINSQGQRVYRFFPWESKVTLV
                     DDFIYTDVSIYKYLMRLKADGEDPEEYKSIWYYF"
     gene            complement(11317..12312)
                     /gene="trpS"
                     /locus_tag="PKZ74_04665"
     CDS             complement(11317..12312)
                     /gene="trpS"
                     /locus_tag="PKZ74_04665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPM92083.1"
                     /translation="METVVSGIRPTGNLHLGNYFGALQNFVKMQNEHHCYFFIADYHS
                     LTTHPTPSDLHGNVKQVLVEYLASGLDPEKATLYIQSDLPETAELYLLLNMNAYVGEL
                     ERCTSFKEKIRKQPENINAGLLTYPTLMAADIIIHRATKVPVGKDQEQHLEMTRTFAR
                     RFNRMYNHEYFPEPIAFNFGDNLVKIPGLDGSGKMGKSEGEGNAIFLADEPGVIRKKV
                     MRAVTDAGPTEKNQAKPEPIGNLFQLMKVVSKPETVAFFDEQYNNCSIRYGDLKKQLA
                     DDMVSFTEPFREKIKELSGNDAYLHKVAMLGRDKARESARKTISDIRKIIGFREF"
     gene            complement(12366..>12796)
                     /locus_tag="PKZ74_04670"
     CDS             complement(12366..>12796)
                     /locus_tag="PKZ74_04670"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92084.1"
                     /translation="FNANLEDDTLYTQPMTYDSLCPYEIQSDTMDLDCDLFVDINELP
                     TREEYESTVKISPNPAEDWVALTLPDIVAAGKVEITVYDVFGREVETGRGGDKEMGGQ
                     GEVPVNRMVVLDVSGYPLGMYVVVVTDRKGRRYTGKVIVN"
BASE COUNT         3127 a         3105 c         3144 g         3420 t
ORIGIN      
        1 gctcgattcg gaaacgccat attccggcgg catcgtggtg atcgtcagtg aaaccatgta
       61 aatgcccgat gcatcgtaga tatggtcagg atcctggacg gtggaagact tgccatcacc
      121 aaaatcccag taccacgact tgatgctgct gccgatcgac tggtccctga aaatgatctt
      181 catcgggtta tcgtcgaggg tatccggggt tggtataaaa ttagcctgga ggtccgggtt
      241 ggcttccgga tcgaaaatgg cgaaatcggc gaacatcagg tactggtttt cccagatgaa
      301 ccggtagtac cggtcgagtt cgatcctgtt attatcaaaa tcatacacat agatcttgat
      361 aatcccgtcg ttagtagtag tggtaagggt atcccagtaa aaaccattga catcggtcct
      421 tgttacggca taatagccaa agcctccgtt ggtcagtgaa tcggaggtaa tgtatatttc
      481 atggtcggcg atgggcgccc ctgtattggt gctggtcagc tgtccgctca gacagatgtt
      541 cttttgcccg gcgatgatct gggatgaaac cggaaggacg atgaaactca tcagcgtcac
      601 aacggcggcg cagatgatgg ccttcgtgcg gtattgcttc tgaatgatat tcattatcct
      661 tttcatgatc aattatttaa acctgtaatt caggcctgcc cttattccga acgaatgcgt
      721 cctgccgttc ctgttttcag gcatggtgaa atcgggatcg agggcataac ggaacgtggg
      781 ttcaaggctg aatattactc tgtcggccag ttcgtaatca aatccggcgc ccacgacgag
      841 ctgccagttg atggtcgaac ggaccggtat cagggaattg acattgacta ttcttgcatt
      901 ctcttccggc acactggcag cagggatatc tttcagaatg aggaaggaag cagccgggcc
      961 ggcttgtgcc ctgattccga accttccgaa cgaatgcctg taaccaaaca gcaccgggac
     1021 ctccagatag gtataggtgg cttttgtttc ggagatgctg tagtgatcca ctgtatcata
     1081 tacatcgact gtctgggtat gatagatagg gataacacca ttttcagttg aatcgaaggt
     1141 tacttcgtaa acatcttcat aagaccccaa ataacggttg aaatcgatgg cataatctcc
     1201 tttatcgtgt gtaaaccggg cgttgaatcc gctctgaaca tagaagtggc tgaagctgat
     1261 agtcggcagg attgccaggt taaatccgat tgaattttcc aggctctcat cgctgtagct
     1321 ggtcatttcc gggttgaaat atgcaccgag cgaccatgtc ggttttacat tacgttgttt
     1381 ttcagctatg accgacaatt tcctgatttc tctcggggtc aggtcatatc tcatcgcgtc
     1441 ggcataagtt atggtaccga gggtaacggc cctgataatt cccgggcggt atccggcaac
     1501 cggttctttg cggagtttcg gtgttgtttc gacggttcgt gtctcttttg atttcctgct
     1561 gaatttggtg ttttcaccaa acggagccct gacaggaata tccggaacct gctttttggc
     1621 ttcggctttg actttctttt cttccgggga aggtataatt tccggtgact ggaatgccgg
     1681 ctcaggcagg gtcagattgg cattggaggt cgtccctgcg ggagcaacac cggcggtttg
     1741 tacagtggaa tagggtgaag aggattcgcc ggccgtagta tcgggcctgt cggggtaata
     1801 ctggttgaac agcccggcaa taaaaataat aagcgaaata gtgacaatga taggaagcag
     1861 gatccccggg gatgaaggag gtgcagggtc tttcggcagg ctggacttga tgttctccca
     1921 cacatgcaat ggcggctccg gctcgaagtt gtcgaacttc tgccggatga tctcatcaat
     1981 atgtttaaac tcttctgcca tcacttttac tatttgcgtt gagccagtcg ttcctggagt
     2041 gccgtccttg ccctcgataa ttgtgatttg gacgtattct ccgaaatttt tagcatttct
     2101 gcgatctcct ggtgattata accttcaata acgtaaaggt tgaaaaccat ccggtaacct
     2161 tccggcagcc cttgaataag gtgaagaaga tctcctgctg aaattttatc gaggatatct
     2221 tccgtggttg aattcatgat caccgcttta tcgatcggtg tttcattcaa ttcatgcagc
     2281 ctggaattgt aataattaat agctgtattg acgattactc tccggaccca gccttcgagc
     2341 gaaccgtcgc agcggaaatc tttcagatgt gcaaagactt tgataaatcc ttcctgaagg
     2401 atatcgtcgg cttccagcgt gtttcttgca aaacgaagac agactccgta catgcggcgt
     2461 gaaaaccgct tgtaaaggaa ctcctgggct ctgggatcat tcgccaggca atgttcgatt
     2521 aattccttat cagagtacat cttaactgaa aaacctgctt gaggccagtc tttccaactg
     2581 tctgaagctg attatcctaa tttatctgct acaaacttag taaataaatt atgatacatc
     2641 gcataaacag ctattatttt tctgctgaca cagaaacgca atcgctcaaa gaacaacttc
     2701 aacgcaacat cacaggcata aatttgcccg tggatgcaag gagaagacag aataaaacgg
     2761 gaaagggttg cacgggccag aaaaaaatcc cgggaaaaac actatttcct tttcctcatc
     2821 tcatgaatcc ttatcttacc taggtaaagg acatgataca tagcgggcac gacaatcagt
     2881 gtcaggaagg tgctgaatgt caatccgaag ataatggtcc aggaaatcgg cccccagaaa
     2941 gccacgttat ctcctccaaa atagatcttg ggatcaaaat gctcaaagag ggaaagaaaa
     3001 taaatattca gtcctgatgc cagcggtatc aatcctaaca gagttgttac cgccgttagc
     3061 agaacagggc gcaaacgggt ccggccggcc tggatcacac aatccagcgc aatgtctttc
     3121 ggcaacagcg cgttttcctc cataccgagc tcggtgcgtt ttctcagttt cagcaggtca
     3181 gtgtaatcga tcagcacaat ggcattattg accacaattc ctgcaagaga aaccagcccg
     3241 atccccgtca tgatcacaac gaagtccatt ctgaacgttg ccagcccgcc gaacacacca
     3301 attgtcgaaa ataatacact gatcatgata atgaccggct tgaccaccga attgaactgg
     3361 ctgaccatga tcaggaggat aagcgccagg gcaatcagca tcgcgctgcc cagaaaggcc
     3421 attgattcgt cctgttcctg ttgttcaccg gtaaatttgt aagtgtaacc ggaagggagg
     3481 gtttctcctg acagaaattc tttgagctga ttgtttatat cggtagcgtt gaaacccttc
     3541 aatacgttag aactcaaggt gatgaccctg tccagatcct tacgctttat cgcttcatag
     3601 gtgctgccgt aactgatatc ggccactgcg gtgatcggta cctgctgtat tttcccgctg
     3661 gcctggtccc tgaaggtgat tttctgattc atgaggctcg ccacattgta ccggaattcc
     3721 tccttaagcc ttaattggat tgggtattcc tcctcaccct ccttaaaatc ggaaatctct
     3781 ttcccgaata aggccgtccg tatcgttccc gctatctggg ccgttgataa accatatcgc
     3841 ctcgcccggt cacggtcgat atggacgatg gcttccggct tgtccaggtt gagatcggtt
     3901 ttcaatcctt caatgccgtc tataccggca ttgtcaattt tattgagaag gtcatcgacc
     3961 aggatgacca gtttgtcgaa atcttccccg gtaacctcta tattgatggc tttccctacc
     4021 ggagggccgc tgctgggttt atcgacagcc accattaccc ccgggtattt tccgatcaga
     4081 ttatcggaaa atgcttttaa aatatcgttg gtgttaacgc cttcccggaa ttcaaaatcg
     4141 acgaaggtaa cggtcaccat tcccctgtgc ggcgtttcgg aaatatcgaa cccttcattt
     4201 tcacttttgg ccccgttacc gaccgttgtc aataccgatt tgacgatccg gctgtaaggg
     4261 gtcagaatgc tgtaaacatc ctcttcaaag gtccccataa atttattggt ggcagtaata
     4321 tcggttccta ccggtaaagt agccataata tttatataag aaggatcggg agaggggaaa
     4381 aagatcactt tcggctggcg gattcccatc agtacgatag tgaatattaa caggaaaaat
     4441 gtcgcagcga tcacccagta aggcctcctg gctttcatca caaaattcat ggtgttcagg
     4501 taggcatctt ccagccaggg caagaaaaca ttcatgaacc aacgggccat cctgttcagg
     4561 aagagcaggt tggcaatgcc gatgatggca aataaaacga acagtgaccc gggtgtattc
     4621 atcccgccgg catagaaaat accggcaagc agaagcagaa tagcaacaat gattaaggaa
     4681 cgtttgattt ttggttttgg cagttgatcc ccttgtttca ggaatgagga ggtaagaacc
     4741 ggtgtaacgg tcaaagctac aaaaagggat gcagtaagta cgatgatcag cgtgagcgga
     4801 agcagcttca taaactcccc gatcatgctg tcccagaaca gcagcgggaa gaaagcggcc
     4861 agggtggtgg ccgtcgaaga gatgatggcg atggcaatct cacctgtcgc caggcggctg
     4921 gcttccatcc ggctgtagcc cctgtcgaaa tatcggtaaa tattctccat caccacaata
     4981 gcattgtcga caagcattcc gagcgcaagg atcaatgaga agaggaggat catgttcagt
     5041 ttgtacccca tcaggttcag gatgaggaaa gagatgaaca tcgataacgg gatggcaaag
     5101 ccaacgaaaa gggcattgcg tgtacccagg aaaaggaaaa gaacgaacac caccaggatt
     5161 acccccatga tcatgctgtt ctcaaggttg tcgagctgct tgcggatcag gtccgactga
     5221 tcgttggtga tcgtgatatt cagtccttcc ggtaaattgc cgtcttcctt ggagcggtca
     5281 agcaggtcaa agatcttgtc ggtggccgat aaaaggtttt ctcccccttt tttaatgaca
     5341 tgcagcgtca ctaccggctg ccggttcaac cttgaaaaat ctttgggctc tgcaaacccg
     5401 tcggttacaa ccgcaatgtc tttcaggtaa acaatattcc ctttttccga tttgacgatg
     5461 atgtcgccca tctgggctgc cgtttcaaat tctccgagga tgcggatcga ccggcggtag
     5521 tcgccttcgc ggaactgccc ggctgccatg gagatattct caaacccgac tgcgctctca
     5581 atatcgccaa agctgatctc cagcatctcc attttaaaag gatcgacatt aatactgaac
     5641 tccctctcat ttaatccttt aatatcgact tttgaaattt cggaataagt ttctatttca
     5701 tcttcgagat attcggcata cttcttcagt tcattcaggc tgtaatcacc cgacaggttg
     5761 atgtaaatga tcggaaactc cgagaaatcg atatcctgaa ccagcggatc ggcaggcagg
     5821 tcatcaggca gttcactttt ggctttgtcg acggcatctt ttacatcgag cacggcctgc
     5881 ttcaaatcga cacccgtatt gaattccacg acaatcgaag aaatatcctg ggaagaggtg
     5941 ctggcaatct ctttcactcc ggtaattacc tccagttctt tctcaatggg ccgcgtgaca
     6001 aggttttcca tatcgagcgg agggttcccg gggtacgcgg tctggaccag tacggtcggg
     6061 atcacaatat ccgggaaaag ttcttttggc attgaagtgt aggaataaaa accgaatcca
     6121 aaaaggatca ggatcagcag gtaaatcgag tttttattct taaggaccag gttcgtcagg
     6181 ccgaactgtc ggatgacctt ctggcttttt tccggttgtg aattaacagg cataaatgat
     6241 taacaattca gatgatgaag tcggatttaa gacggtgcaa aagtacgact tggattgaaa
     6301 aattaaattt ttttatcagg cttctacttt ttttcttaac cgggtgctga tggagatcaa
     6361 aagcacaaac acggcaatga aagccgacag cctggcaata taatcgccat atctaacata
     6421 gaaagtggta aaattgttcg cattgatctc ctggcggata acggcaggct cccagtagtt
     6481 ggttctctgg taaatatcgc cacgctggcc gatgaatgca gaaataccgg tattggccga
     6541 gcgggctatg ctgcggcgtg tttcgatggc cctgagttta ctgaaagtaa gatgctgccg
     6601 gtggccgggt gtgttgcccc accagccgtc gttggtaatc acaaaaataa ggttggcccc
     6661 cttgcggatg tattgtgtga catattcgcc gaagaccgat tcatagcaaa tgaccggcgc
     6721 gatcttcagg ctgtcggatt ttcggaggaa gacggtccgc tctttatcgg tccccaggct
     6781 gccgaccgtt ccgccgagat caagggccag gctttccagc ggtttcagca agactgccag
     6841 cggcattttc tccacaccgg gcgtcagttt cgacttatgg tgaagctggt accgcttgtt
     6901 cgtatcaata taaaaagcag tattgaatgc atcataataa ccttcagcgc tgcggaactt
     6961 gcgcgccgtt gccgattttt tctcatcgtc gccgaaacgg taaaaagtgg aagccccgat
     7021 aatataagcc gctgaaggat atcctttaag atattccctt atggaagcca gtccgcgcgc
     7081 atcgtgcagc cggttctccc agatgttctc ctgcagcgcc gattccgggc tggcaataaa
     7141 atcaacccgg ttatccatca ttttttgcgc caggtccagg tttgccttta atagctcggt
     7201 ggattccagc gtgtactgtt cattgtatgg atctatatcc ggctgaacca caataacatc
     7261 cactggccag ggtttttcct cgtatttgtt gtaaaggcga taagacagta agacgggtac
     7321 gacgattaga agtactaaag cacttccttt cacccaattc attttccatt gtccggtgtc
     7381 cattgtccag tttccatttc ctttttccct atttcctttt cctttaaaaa tatcgtaagc
     7441 caaaatgttc actgccagaa tccacagggt tccaccgaag atgccggtgt actcgtacca
     7501 ttggatccac ttgtgccagc cggcaaatcc gttccccagg ctgagccagg gccaggtgcc
     7561 gtcccagttg tgatggaagt attcgaaagc gatccagaaa aagatgagcg gaatttgtcc
     7621 ctgctttcca ggcatagcgc ggcgggtgaa atgaaagatc cagaaaatgg cggccatgaa
     7681 cagggcattg agaacccagg ccatgatggc accgaagtcg gtagaattcc atatccacca
     7741 ggtagtcagt atattccaga ggagaaatcc cgggtaggtg tagagaaaaa cacttcgttt
     7801 tctgaacttg tcctggtgat caaaaatgta atcttcgatg aaaagaaagg ggataaaacc
     7861 gatgaaaaga agcggtgaga atcctctttc gggccaggcc aggctcagga ggaggcctcc
     7921 ggctatagaa atgctgatga gatggatttt cttcataaaa aacgataaag gtaacggctt
     7981 ttcatgatca aatcgtatcc aaaaccgatt ttcaaaactc ttcaaacagg aagttattaa
     8041 acggatagat ttttgtatgg atcttcctga cctccccgta taacatgttt ttgaattcct
     8101 ccaggttaac tttttgtgtt gctgaaagaa agatcgaggg gtaatgactg ttgctcatcc
     8161 aggttttttt caggtcatcc aggctcaggt tctctttacc ggcaggggtg aggtcgaacg
     8221 ggtctttctc cacgaaggtg taagcatcga tcttgttgaa cagcataata accggcttat
     8281 tgctggcacc gatctccgac agggtctggt tgactactgc gatctgctct tcgaaatggg
     8341 gatgcgaaat gtcggcgata tggacgagca ggtctgcttc gcgcacctca tcgagcgtcg
     8401 atttaaacga ttccaccagg ctgtgcggca gtttccggat gaagcccaca gtgtcggtca
     8461 gcaggaacgg caggttgccg atcaccactt tccggacggt tgtgtcgagg gtggcgaaga
     8521 gcttgttctc ggcgaatacg tccgatttgc tcagcaggtt catgacggtt gatttgccaa
     8581 cattggtgta accgacaagg gcgacgcgca ccagctcttt ccgccctttt cgctgcgtgg
     8641 tcttgatctt atcgatctcc ttcagctttt ctttgagaag ggcaatccgg tcgcggacga
     8701 tccggcggtc ggtttcgatt tcccgttccc ccgggccgcg caacccgatg ccgccctgct
     8761 gccgctccag gtgggtccac atccgggtca gcctgggcag gagatattcg tactgcgcca
     8821 gctccacctg tgtgcgggcg tgggcagtcc gcgcacgtcg ggcaaagata tccaggatca
     8881 ggctattccg gtcgagcacc ttaattccca gctctttctc cacgttccgg gactgggagg
     8941 gggtgagttc gtcgtcgaaa atagcgatgt tgatatcctc gtttttaacg aattctttga
     9001 tctcgttgag tttcccctgg ccgatgtagg tccgctggtc gggcaggtcg attcgctgga
     9061 tgaaccgttt gacggccact ccgcctgcgg tatcaaccag gaaagccagt tcgttcagat
     9121 aatcgttgat ccggtcggca tcaaagcggt ttttaaccag tccgatcagg acggcccgtt
     9181 cgacgaccgg ttttatgata attccttttg gcattcaaac agggttggaa acccataggg
     9241 ttggaaaccc ttttccgatt atccggcaaa gataatcaga aataatacca aatggacttg
     9301 tactcttccg ggtcctctcc atctgccttt aatctcataa ggtacttata aatagaaaca
     9361 tcggtataga taaaatcatc caccagcgtc actttggatt cccagggaaa aaacctgtaa
     9421 acccgctgac cctggctgtt gatcatgatg agttcatggt cctgccaggc cctcaggtgc
     9481 gatttgccca gcttcggcac attgaaaacc ggttcatcgg tgcgggccag cccgggcagg
     9541 aaacgggcct cctctttccg ttcctggctg atacgagcta tgggaaccct gaaatcggat
     9601 gtctcctctt tacctttcgt gttgaaggta taataaggat ccaccccggc caacttaagc
     9661 tgttttcgaa gcgcacacga tgcaaaccgg gaactgttat aatacgtgaa aacctgctga
     9721 ttgtatatgc tgatacccgc ccggcgaatt ctcccaatgg cagaaaccgc ttcgggagtg
     9781 atctccatcg gatgctcaaa atgagtgaca atgcatatct cacgttttcc gggccggtga
     9841 aatttcctga gaatccggat aaagctatcg ccaatccgca tcggcagtgt gaccagaacc
     9901 cgcgttccga tcctgatcct ctctacatga ccgatttcag acagcgattg cagaatggtc
     9961 tcaatgtaat catccccgag tattaatggg tcgccgccgg ttaccagcac ttccgaaatg
    10021 ttttcgttgt tccggatcca gtcgattgcg gcagcggttt tatttcgtgt cgccttcgat
    10081 tccctcatgg gtttaacctc ccagttgcgc tggcaataaa cgcaaagctg ggggcagata
    10141 tcgacaggtt tgagaataac gatatgcgga taacgccggg tgatggcttc tgccggactg
    10201 gttgatcgtt cacccataaa atccatatcg aagcccgact tcctgttttt gtcgacgttg
    10261 atgcaataat tcgcactggg cagcacctgg gccctgatgg cgcggtcatc ttttgtcctg
    10321 cctttccggt taaaaagcga gagatagtat ggagtaattt gaaacggtat gcggtatctt
    10381 tctgcatatt caagcccaag ccgctcatcc tcatccagca taaccagctt ttcaagcgtt
    10441 ttccgcgtcg tgatgatgtg tttgagttgc catttataat cctgccaatc cttctctgtg
    10501 ccctggtaat actcaagtat cgccttgctg gccgcttttc tgtcatgaat cacagccgga
    10561 tcaagaccat ggcggtaatg ccggagatga tcatccattc gccgggcata ttcatccagc
    10621 tttttcgacc tgagctgcga agcttttttg ttgtcggaag gtagtttaaa gctgtcatcc
    10681 gtaattcctg accgggcatg cactccatga aaaagaaaga tgaactctgc cagaaaactt
    10741 tcggaaacag gaatttccgg ctcactattc agtgcaagat cgcggaggac ctttagcgct
    10801 gagaaatctg caattttctc attttctgtt cggatcacgt ttttaaggac ccggatgcat
    10861 tcttttgcat ttgttttttc tatgatgtgg aaatctttgt attccttctc cgaatgaatg
    10921 ttgaaataat gcctttccag tccgttcaga tattcaaaga gattctccct ggcatcggcc
    10981 acatcagatg attttttcag tagggccctg atttccgggt tggcctccca taattttttt
    11041 ttgagttgat ctttcgataa tgatgctatt ttattcattg ttaatgaatt ttaaatgatt
    11101 gatccctgtg tgaaccagct ttctgctgaa ataaaatcaa tgataacggg gaaaaagaat
    11161 aaaactggta gtcatccgaa gacagatgtc caaagatgct aattagatgt caaataatca
    11221 ataagataca agatctgttc agcattaacc cgttattgaa cggctaaaaa taagaaaaaa
    11281 aattgaaata gccaaaaaaa gggttggaaa ccctatttaa aactccctga aaccgatgat
    11341 ctttcggatg tcggaaatgg ttttccgtgc actctcacgg gctttgtcgc ggcccagcat
    11401 ggccaccttg tgcaggtagg cgtcgttgcc cgaaagctct ttgatctttt cacggaaagg
    11461 ttcggtgaac gacaccatgt cgtccgcaag ctgcttcttc aaatcgccgt agcggatcga
    11521 gcagttgttg tactgctcgt cgaaaaaggc gacggtttcg ggtttcgata cgactttcat
    11581 cagctggaac aggttcccga tcggctctgg ctttgcctgg ttcttttcgg tcgggccggc
    11641 gtcggtcacg gctcgcatca cctttttccg gatcacgccg ggttcgtcgg ccaggaagat
    11701 ggcgttgcct tctccttccg atttgcccat cttgccgctg ccgtcgagcc ccggtatctt
    11761 caccaggtta tcgccgaagt tgaaggcgat cggctcgggg aagtactcgt ggttatacat
    11821 ccggttgaag cggcgggcga aggtgcgcgt catttcgaga tgctgctcct ggtctttgcc
    11881 caccggtact ttcgtggcgc ggtgaatgat gatatcggcc gccatcagcg tgggataggt
    11941 gaggaggccg gcgttgatgt tctcgggttg tttgcggatc ttttccttga aggaggtgca
    12001 cctttccagt tcgccgacat aggcattcat attcaggagc aggtagagct cggccgtttc
    12061 gggcaggtcg ctctggatgt agagggttgc cttctccggg tcaaggccgc tggccaggta
    12121 ttccaccagc acctgcttca cattgccgtg caggtcggac ggggtggggt gggtggttaa
    12181 cgaatgataa tcggcgataa agaaatagca atgatgttcg ttctgcatct tgacgaagtt
    12241 ctgcagagcg ccgaaataat ttccgaggtg aaggtttccg gtaggccgga tgccactgac
    12301 aactgtttcc atggcgcaaa gatagtgaaa ttagtcttcg gtcggcagtt agcggtcggc
    12361 agtcgtcagt tgacgataac cttccccgta tatcgcctcc ctttcctatc cgttaccaca
    12421 acaacataca tccccagcgg ataacctgac acatccaaaa ctaccatccg gttcaccggt
    12481 acctctcctt gtcctcccat ctccttgtct cctcctcttc cggtctccac ctcccttcca
    12541 aacacatcat acacagttat ttccactttc cctgcagcta ctatatccgg caacgtcagc
    12601 gctacccaat cttcggccgg attcggcgaa atttttaccg tcgattcgta ttcttcccgg
    12661 gtaggcagtt cattgatatc tacaaaaagg tcgcaatcca ggtccattgt gtccgactgg
    12721 atctcatacg ggcacaggct atcgtacgtc atcggctggg tgtagagggt gtcatcttcg
    12781 aggttggcgt taaatt
//
LOCUS       DAOPNH010000059        12740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5087_length_12740_cov_5.543319, whole genome shotgun sequence.
ACCESSION   DAOPNH010000059 DAOPNH010000000
VERSION     DAOPNH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5087_length_12740_cov_5.543319"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKZ74_04675"
     CDS             complement(<1..233)
                     /locus_tag="PKZ74_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HPM92085.1"
                     /translation="MDEKLYNEIVAEENLVYKKTSLMTEKVLSYCPGCGHGTAHRIIM
                     EVLDEMGLQENTIGVAPVGCSVLAYEFMNVDMQ"
     gene            complement(238..1320)
                     /locus_tag="PKZ74_04680"
     CDS             complement(238..1320)
                     /locus_tag="PKZ74_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPM92086.1"
                     /translation="MGELKLMKGNEAFAEGAIRAGADAYFGYPITPQSEVLEYFMAEK
                     PYEKTGMVVLQAESEVAAINMVYGAAAAGKRAMTSSSSPGVSLKMEGISYMAGAELPG
                     LILNVVRGGPGLGTIQPSQADYFQATKGGGHGDYRLIVLAPSSVQEMFDFVSLSFELT
                     FKYRNPVMMLSDGVIGQMMEKVQLSEPKPRRTDEEVIRECPWATTGKPKTRERNIITS
                     LNLDSAVQERHNLHLQEKYRKIEEHEVRFEEFQSEDAEYLFVAYGSSARIAQKAVQLA
                     RAKGIKAGLIRLITLFPFPKKRIGELGGKVKGILTVEMSAGQMVEDVLLAVAGKVKVE
                     HYGRMGGMIPSPEDVVTAMEKKIIGG"
     gene            complement(1343..1573)
                     /locus_tag="PKZ74_04685"
     CDS             complement(1343..1573)
                     /locus_tag="PKZ74_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM92087.1"
                     /translation="MAKVRGVIIVDIERCKGCEVCVGSCPTEVIAMAREVNHKGYHYA
                     FSANPDECTGCTNCAVVCPDGVITVYRKKVEA"
     gene            complement(1727..3040)
                     /locus_tag="PKZ74_04690"
     CDS             complement(1727..3040)
                     /locus_tag="PKZ74_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM92088.1"
                     /translation="MKNTPIDFNVVDKYLNETRIGDLGIASIREIKRLVDLIEKESGQ
                     RYVRMEMGIPGLPPVKIGIEAEKEALDMGIPAIYPDIYGRAELKKEISRFVKLFLNLD
                     VHVDGCIPTVGSMQGSFATFMTLNRMHKDKGQTLLINPGFPVHMQQLRILGIETLSFD
                     VYDYRGDKLRDKLESYLSSGKVSTILYSNPNNPSWICFTDKELRIIAEMADKYNVIII
                     EDLAYFGMDFRHDYSQPGKPPYQPSVAHYTDNYVLLISSSKVFSYAGQRIGMMVISNK
                     IFHTEAPDLLRFYKFSKVGHAMIFGTLYSLSSGTAHTPQFALTAILKAANDGQFNFVE
                     EVKEYGEKARIMKKMFTDNGFRIVYDKDEDKPIADGFYFTFSYPGMTGGEMLHELLYY
                     GISAIALSITGSERTEGVRACVSLVRRDQFPDLEKRLKQFHEDHL"
     gene            complement(3117..5177)
                     /locus_tag="PKZ74_04695"
     CDS             complement(3117..5177)
                     /locus_tag="PKZ74_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPM92089.1"
                     /translation="MVNRELTDPKSIVIVGGSNDLQKPGGKILKNIIDGGFNGTLMVM
                     NPKEDIVQGVKSYRNATDLPPVELAVMAIASRHIPDTAEFLANEKGTRAFIVISAGFS
                     EENEEGKALEKKLVEIVNKANASLIGPNCIGVLTPAYHGVFTLPIPKLSLSGCDFISG
                     SGATACFIMENGIQKGLAFARVFSVGNSAQMGVEDILRHMDESFDPAISPRVKLLYME
                     SVQKPEMLLRHASSLIRKGCRIAAVKAGASEAGSRAASSHTGALASSDLAVSCLLRKA
                     GIIRCQGREELINVASVLMHKELQGKNIGIVTHAGGPAVMLTDALSREGFSVPRIENP
                     KATELLQQLYPGSSVSNPVDFLATGTAEQLGTILDYTDKYFDEIDGIAVIFGTPGLNP
                     IFDVYDVLHKKMQNARKPIYPILPSTYTASEEVRDFLSKGRINFPDEVLFAHALGLSY
                     FTPKPAEIQLIDEAQAPAGLTQKVGSNGEGYLPPAAVAEMLDFAGIPRVPEVTVRQPE
                     EIAGVLKNIGIPVVMKVIGPVHKSDLGGVILNIRELDQAIDAFGKLMIIPEATGVLIQ
                     PMLKGMELFAGIKHEVNFGHILLCGMGGIFIEVLKDFSASLTPVSKAEADEMIRNLRI
                     YPMLKGVRGQKGINLELFSEILVRLSSLVHHFPGITELDLNPLMASGDKIYAVDARIR
                     IGHT"
     gene            5322..5783
                     /locus_tag="PKZ74_04700"
     CDS             5322..5783
                     /locus_tag="PKZ74_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPM92090.1"
                     /translation="MKRTALFIFLVMVSAGIFAQATEPVKAEENPNAPEITFDKLVHD
                     YGTIAFGGDGVSVFKFTNTGKEPLILQQPQSSCGCTVPTWPKEPILPGNSSEIQVTYN
                     TKKAGPINKTVTVRSNAKNNTVVLRITGKVEADPATQMPENKPGEGTPVNK"
     gene            5866..7065
                     /locus_tag="PKZ74_04705"
     CDS             5866..7065
                     /locus_tag="PKZ74_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM92091.1"
                     /translation="MPKISKKGMMMPESPIRKLVPYAETAKKAGRKVYHLNIGQPDIE
                     TPEVALNAVRNFSQKVVEYSHSAGILSYRQKLTEYYKKINIDITPEEIVVGAGASEAL
                     LFAINSCLDPGDELIIPEPFYANYNGFSINCGAVVHPIVSSIDSGFALPPMADFEKAI
                     NEKTKAILICNPNNPTGYLYSKEELEILRDIVKKYDLYLITDEVYREFVYDGHHHYSA
                     MHLEGIEQNVILIDSVSKRYSACGFRVGCLITRNKDVARTALKFAQARLSPPTFGQVA
                     AEAAIDTPSEYFVRVLDEYHARRNIVVEYINKIPGGFCPNPKGAFYVVARLPIDDSDR
                     FCKWLLEDFNFNNETVMLAPATGFYSNPSRGKDEVRISYVLKVEDLKKAMVCLEEALK
                     VYPGRTL"
     gene            complement(7076..8326)
                     /locus_tag="PKZ74_04710"
     CDS             complement(7076..8326)
                     /locus_tag="PKZ74_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPM92092.1"
                     /translation="MITLRKTRAIRMMIHQYILIVLSILWMAFSGIQTGNAQPSQSTA
                     IRQVPYTAIRTGAERSDVYVPLLKGKRVGIVCNQNSRIGTTHLVDYLLESGVKIEKIF
                     APEHGIRGDVEAGGSIDHGTDERTGIRIVSLYGTNKKPAQQDLEGIDLIIFDIQDVGV
                     RFYTYISTLALVMEAAAENDLPVVVTDRPNPNGFYTDGPVLDTNFKSFVGMHPVPVVY
                     GMTIGEYALMVNGEGWLGSRKCELTVIPMEGYDHNMLVTLPFRPSPNLTTWESIYLYP
                     STCFFEGTIVSVGRGTLGPFQVFGHPGFLIGSYKFTPVKIPGMAEKPLFEGEECLGHE
                     VTEYAHDFLNHPNQLNLFWLIESYRYFSGRADFFNSYFDKLAGTDRLRLDILSGKGED
                     EIRESWEPGLSAFRAIRERYLIYP"
     gene            8384..9610
                     /locus_tag="PKZ74_04715"
     CDS             8384..9610
                     /locus_tag="PKZ74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92093.1"
                     /translation="MNLPSFIANRISGKDKENLSGPAVRVSVFTIALGLAIMILAVSV
                     LVGFQKEIRNKVIGFSAHIQIDNFDDNTSFESQPIPADQPFYAELKGTPGIRHIQVFG
                     LKAGIIKTQDQLQGVILKGVGPDYDWNFMKNHLAEGEIITLEDSAASSGILISRLMAS
                     KLKIIPGDEVRMYFVSGSDSQPRGRKFTVAGIYETGLEEFDEMYVIADIRHIRRLNNW
                     EPGMVSGFEVFIDDFDNIDEMGEKVYQTIGYDLNAETIVQKYPQIFDWLRLMDINVVI
                     ILVLMILVAGITIISTLLILILERTSMIGILKALGTKDRNLRKMFIFLTGRIIAKGLF
                     WGNLAGVGIGLLQFYFRIIPLDQESYYMAYVPVSLMLWQILLINLVTVVTCLVMVLIP
                     GYIIGRITPVKAIRYR"
     gene            complement(9617..10330)
                     /locus_tag="PKZ74_04720"
     CDS             complement(9617..10330)
                     /locus_tag="PKZ74_04720"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPM92094.1"
                     /translation="MEKEFAISYADKLNRDFADRAPEDIIAYIIENHRDRAAFSTSLG
                     LEDQALTAMIARIDPGFPIFTLDTGRLFAETYDLIDLTSLRYNVKIKVYFPDNQKVEE
                     MVSSRGINLFYDSVENRKLCCHIRKIEPLQRALTGVDFWISGLRREQSVTRENLTLAQ
                     YDPLHHKIKINPLINWTYEQVQSFIKINKVPYNPLHDKGFPSIGCQPCTRAVEPGDDI
                     RSGRWWWENPEMKECGLHK"
     gene            10383..11765
                     /locus_tag="PKZ74_04725"
     CDS             10383..11765
                     /locus_tag="PKZ74_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM92095.1"
                     /translation="MDKRMVIMKVHFIAIGGSAMHNLAIALKLKGYRVTGSDDEIFEP
                     AHGRLGKYGILPEKEGWDPAKITPDLEAVILGMHAKGDNPELAAARTLGLRIYSYPEY
                     LYEASKDKIRVVIAGSHGKTTITSMILHVLQRVGIETDYMVGAQLEGFEVMVKISDSA
                     RFMVLEGDEYLSSTLDPRPKFHLYRPDIALLSGIAWDHMNVFPTFENYISQFSRFLEC
                     FEPRGKLVYCESDPVVASISESSRKDIEKIPYGNLDYIIKEKKTRLLVNGLEFPVNVF
                     GRHNVQNIHGAWLICRQLGVSDEEFLMAIESFSGASKRLQILAESPDCNVYKDFAHSP
                     SKLKATTLAVKELFPERKLVACLELHTYSSLNKDFLPLYKGCMDAADIPVVYFSHHAL
                     ALKRLPELTTQDIRDAFSLDRLMVFNDSLKMKEFLLSLDLTNKNLLLMSSGNFDGLDL
                     DDLAEKILPV"
     gene            complement(11790..>12740)
                     /gene="rho"
                     /locus_tag="PKZ74_04730"
     CDS             complement(11790..>12740)
                     /gene="rho"
                     /locus_tag="PKZ74_04730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPM92096.1"
                     /translation="IRPPKEGEKYFPLIKVEMINGRKPEEVRDRIPFDFLTPLFPLTK
                     FKITDYPNASITTRVIDMFSPIGKGQRGLIVAQPKTGKTIILQEIANAIAYNHPEAYL
                     IVLLIDERPEEVTDMQRHVKAEVIASTFDEPAERHAKIANIVLEKAKRLVECGHDVVI
                     LLDSITRLARAYNTVSPASGKVLSGGVESNALQKPKRFFGAARQIENGGSLTIIATAL
                     IDTGSKMDEVIFEEFKGTGNMELQLDRKLSNKRIYPAIDIVASSTRREELLVDKESLQ
                     RIWILRNHLADMNPLEAMEFLKDKMKYTRNNEEFLISMNS"
BASE COUNT         3162 a         3115 c         2986 g         3477 t
ORIGIN      
        1 tgttgcatat ccacattcat gaattcatag gccagaacgg agcaaccgac cggtgcaacg
       61 ccaatggtgt tttcctgcaa acccatttcg tccaacactt ccatgatgat ccggtgggcc
      121 gtcccatgcc cgcatcccgg gcagtagctc agcactttct cggtcatcag gctggttttc
      181 ttataaacca ggttttcttc ggctacgatt tcgttatata atttttcgtc cataatttca
      241 acctccgatt atttttttct ccatagcggt cactacatct tccggtgatg ggatcattcc
      301 gcccatacga ccgtaatgtt caaccttaac cttgccggct acggccagca atacatcttc
      361 aaccatctgg ccagcgctca tttccactgt cagtatccct ttgacttttc caccgagctc
      421 gccgatcctc ttcttcggga acggaaacag cgttatcagc cggatcaggc cggctttgat
      481 accttttgcg cgggcgagct gaaccgcttt ttgagcgatc cgtgcactgg aaccgtatgc
      541 aacaaacaga tattctgcat cttctgactg aaactcttca aagcgaacct catgctcctc
      601 gatcttgcgg tacttttcct gaaggtgcag attgtgcctt tcctggacag ctgagtcgag
      661 gttcagcgag gtaatgatat ttctttccct ggttttgggt ttgccggttg ttgcccatgg
      721 gcactccctg atcacttctt cgtcggtccg gcgtggtttc ggttctgata actgaacttt
      781 ttccatcatc tgcccgataa cgccatccga aagcatcatg accggattgc ggtatttgaa
      841 tgtcagctcg aaagagaggc tgacaaagtc gaacatctcc tggaccgaag aaggagccag
      901 gacgatcagc cggtaatccc catggccacc gcctttggta gcctggaaat aatcggcctg
      961 agacggctgg atcgttccca ggccggggcc accgcgaaca acgttgagga tcaggcccgg
     1021 taactcggcg ccggccatat acgagattcc ttccattttg aggcttaccc cgggactgga
     1081 tgaggaggtc atcgcccgtt tgcctgctgc tgccgctcca tataccatgt tgatggctgc
     1141 tacttcactt tcagcctgca ggacgaccat gccggtcttt tcatacggct tttcagccat
     1201 aaagtattcc aatacttccg actgaggtgt gatcgggtag ccgaaataag catccgcacc
     1261 cgccctgatt gcaccttctg caaaggcttc attgcctttc atcaatttta attcacccat
     1321 atcttctttc tcttttttgc gatcaagctt ctactttctt ccggtatact gtgatgacac
     1381 catccgggca aacgactgca cagttcgtgc aaccggtaca ttcatccgga ttggcggaaa
     1441 atgcataatg gtaaccctta tggttcacct ccctggccat cgcaattact tcggtcggac
     1501 acgaaccgac gcagacttcg catcctttac acctttcaat atcaacaatt attactcctc
     1561 tgacttttgc catttcctta aaatttaatt gtacacctat tataaataaa ggcgctacga
     1621 aattattaat aaaagatgag ataccggatc agttcacttt tgatttgtga aaagcttaac
     1681 aatttatatt tggtttaaat aattagcttc tcaccaaatt ccgggatcaa aggtgatctt
     1741 catggaattg tttaagcctt ttctccaggt cagggaactg gtcccttctc accagcgaaa
     1801 cacaagccct gacgccttcc gtcctctcgc ttcctgtgat tgatagcgca atggcactga
     1861 tgccataata caataactca tgcagcatct ctcctccggt cataccggga tacgagaacg
     1921 taaaataaaa tccatcggca atcggtttgt cttcatcctt atcatacaca atcctgaacc
     1981 cgttgtcagt gaacattttt ttcatgatcc tggccttttc tccgtactct ttcacctctt
     2041 ccacgaaatt gaactggccg tcgtttgcag cctttaatat ggcggtaaga gcaaattgcg
     2101 gggtatgggc cgttccggaa ctgagtgaat acaaagtgcc gaaaatcatg gcatgtccaa
     2161 cctttgaaaa cttatagaac cttagaagat caggggcttc cgtgtggaag atcttattgg
     2221 aaatgaccat catcccgatt ctctggccgg cgtagctgaa tactttggaa cttgagatca
     2281 gaaggacata attatccgta taatgagcaa ccgagggctg gtagggcggt ttgccaggct
     2341 gcgaatagtc gtgcctgaaa tccatcccga agtatgccag atcctcaatg atgataacat
     2401 tatatttatc ggccatttcg gcaattatcc ggagctcctt gtcggtaaaa caaatccatg
     2461 acgggttatt cgggttggaa taaaggattg tcgatacttt gcctgaactc aggtaggatt
     2521 ccagcttgtc gcgcaattta tcaccccggt aatcgtaaac atcaaaagaa agcgtttcaa
     2581 tgccaagtat cctcaactgc tgcatatgca ccgggaatcc cggatttatg agcaatgtct
     2641 gccctttgtc tttatgcatc cggtttaggg tcataaatgt ggcaaaactt ccctgcattg
     2701 agccaactgt cgggatacag ccatcgacat gaacatccag gttcaggaat aatttgacaa
     2761 agcgggagat ctctttcttt aattccgccc tgccataaat atccggatag attgccggga
     2821 tacccatgtc aagtgcctct ttttcagctt ctatgccgat tttaaccgga ggaaggcccg
     2881 gaatccccat ttccatcctg acataccgtt gccctgactc tttttcgatc aggtcgacca
     2941 gtcttttgat ttcacgtatg gaagcgattc ccagatcacc aatccgcgtt tcattcaggt
     3001 atttgtcaac aacattaaaa tcgatcgggg tgtttttcat aataatttca gttgatatcg
     3061 tattaaatct tttcggttca aatatattaa tttatatcac aaagccaatt tttcaattaa
     3121 gtatggccta ttcgaatacg ggcatccacg gcatatatct tgtcacccga agccatcagc
     3181 ggattcaggt caagttcggt gatcccgggg aagtgatgga ccagtgatga cagacggacc
     3241 aggatttctg agaatagttc caggttgatc cctttttggc cgcgtactcc ctttagcata
     3301 ggatagatcc tgaggttccg gatcatttca tccgcttcgg cttttgagac gggcgtgagg
     3361 gatgcgctga aatccttcaa tacctctata aatatgcctc ccatcccgca caggaggata
     3421 tggccgaaat tcacttcatg ttttatcccg gcaaacagtt ccataccttt gagcattggc
     3481 tggatcagaa caccggtggc ttccgggata atcataagct tgccaaatgc gtcaatggcc
     3541 tggtccaatt cccgtatatt gaggatcacg ccacccaggt ctgatttatg aaccggcccg
     3601 ataaccttca ttacgacagg gattccgata tttttcagaa cgccggcaat ctcttcgggt
     3661 tgcctgacag tgacctcagg caccctggga attccggcaa aatcaagcat ttctgctacc
     3721 gccgccgggg gcaggtagcc ctcgccattt gatcccactt tttgcgtcag cccggccggg
     3781 gcctgtgctt catcgattag ttgaatttca gcgggttttg gggtaaagta agataaaccc
     3841 agggcgtgtg caaacaaaac ctcatccggg aaattgatcc ggccctttga aaggaaatcc
     3901 ctcacctctt cactcgctgt atatgtagaa ggtaaaatcg ggtagatcgg ttttcgtgcg
     3961 ttttgcatct ttttatgcag cacatcatag acgtcgaaga tcgggttgag ccccggcgtg
     4021 ccgaatatta ccgcgatacc gtcgatctca tcgaagtatt tatctgtata gtcaaggatt
     4081 gtgcctaatt gttccgccgt gcctgtagcc aggaaatcaa ccgggttact gaccgatgat
     4141 ccgggataga gctgctgcag taattccgtc gctttggggt tctctatccg gggaacgctg
     4201 aacccttccc gggatagggc atctgtaagc atgaccgccg gcccgcccgc atgcgtcaca
     4261 atgccgatat tctttccctg cagttctttg tgcatcagca cagaggcaac gtttatcagt
     4321 tcttcccttc cctggcacct gatgatgcct gctttgcgga gcaggcagct aaccgccaga
     4381 tccgaactgg ccagcgcacc ggtatgcgac gaggctgccc ggctgccggc ttctgaagcc
     4441 cctgccttga ccgccgcaat cctgcatcct ttccttatca gcgatgaggc atgcctgagc
     4501 aacatctccg gcttctgaac cgactccata tacagcagtt tcaccctggg gctgattgcc
     4561 ggatcaaatg actcgtccat gtgcctgagg atatcctcaa ctcccatctg ggcactgttg
     4621 ccaaccgaga aaacccgggc aaaggccagc cctttctgga ttccgttttc cataataaaa
     4681 caggccgttg ctcctgatcc ggatatgaaa tcacaaccgg aaagactgag ctttgggatc
     4741 ggaagggtga aaacgccatg ataagctggt gtcagcacac cgatgcaatt gggaccaatg
     4801 agtgaggcat ttgctttgtt gacaatttcg acaagtttct tttccagggc ctttccttct
     4861 tcgttttctt cgctgaatcc ggctgagatc acgatgaaag cgcgggtacc cttctcattg
     4921 gccaggaatt cagcggtatc ggggatatgc cgcgaggcaa tagccatcac cgccagttcg
     4981 accggtggga gatctgttgc gttcctgtag gatttaacgc cctgcacgat gtcctccttc
     5041 gggttcatta ccattaatgt gccgttaaag ccaccatcga taatattctt caggattttg
     5101 cctcccggtt tttgcagatc gttggaaccg ccaacaatca cgatgctttt cggatccgtt
     5161 aattccctgt ttaccatcaa gaaaaagatt attgttattt gtttaacatt actgcgctaa
     5221 agtataaaaa tgccatgtgt taaaaaagta taaaaagtaa ttgtaccgcg atattttttt
     5281 aaataattaa tacttttgtc tgtcaataaa aatttataaa aatgaaaaga actgcattat
     5341 ttattttcct ggtgatggtt tcagccggga tttttgcaca ggccactgaa ccggtaaaag
     5401 cagaggaaaa tccaaatgct cctgaaataa cctttgataa actggttcat gattatggaa
     5461 ccattgcttt tggcggggac ggtgtcagtg ttttcaaatt cacaaatacg ggaaaagaac
     5521 ccctgatcct gcagcagccc cagtcatcct gcggatgtac ggtgccaacc tggcccaagg
     5581 aaccgatcct gcccggaaac tccagcgaga tccaggtgac ttacaatacc aaaaaagccg
     5641 gaccgatcaa taaaacggtg acggtaagat caaatgcaaa gaataatacg gttgttctca
     5701 ggattaccgg aaaagtggaa gctgatccgg ctacccagat gccggaaaac aagcccggtg
     5761 aaggtacccc tgttaataag tgatttaatt aatttttttt caaggttgcc tgaacaacat
     5821 aggaaatgta attcaggcaa cctttttatt ttaaatgatt taaccatgcc aaaaatttcc
     5881 aaaaagggga tgatgatgcc ggaatccccg atccgtaaac tggtccccta tgctgaaaca
     5941 gctaagaaag ccggccgaaa agtctatcat ctcaatatcg ggcaacccga catcgaaaca
     6001 ccggaagtgg ctctgaatgc cgtcaggaat ttcagccaga aggtggtgga atacagccac
     6061 tcggcaggga tcctgtccta caggcagaaa ctcacggagt actataaaaa gatcaatatc
     6121 gatattaccc ctgaggaaat cgttgttgga gcaggcgctt ccgaagccct tctgtttgcc
     6181 atcaattcct gccttgatcc gggcgatgaa ctgatcatcc cggagccgtt ctatgctaac
     6241 tacaacggat tttccatcaa ttgcggcgcc gtggttcacc cgatcgtctc ctctatcgac
     6301 tcaggattcg ccctgccgcc gatggccgat tttgaaaaag ccatcaatga aaaaactaaa
     6361 gctatcctca tctgtaatcc caataatccg acgggttacc tctattcaaa ggaagagctt
     6421 gaaattttgc gcgatattgt gaagaaatat gatttatacc tgataaccga tgaggtttac
     6481 agggagtttg tgtacgacgg acatcaccat tactccgcaa tgcatctcga aggtattgaa
     6541 cagaatgtga tcctgatcga ctcggtttcg aagcggtatt cggcctgcgg tttccgcgta
     6601 ggatgcctca ttacccggaa taaagatgtc gcgcggacgg cactgaagtt tgcacaggca
     6661 aggctgagcc cgccgacctt tggccaggtg gcagccgagg cagcaatcga cacgccttcg
     6721 gaatactttg tcagggttct cgatgaatac catgcccggc gcaatatcgt cgtcgaatac
     6781 atcaacaaga ttccgggagg tttctgcccg aatccaaaag gggcgtttta tgtggtggcc
     6841 cgcttaccga tcgacgattc cgaccggttc tgcaaatggc tccttgaaga ttttaatttc
     6901 aacaacgaaa cagtcatgct ggcaccggct accggcttct attcaaatcc atcgcggggc
     6961 aaagacgaag tgaggatttc atatgttctc aaagtggaag atctgaagaa agccatggtg
     7021 tgcctcgaag aagccctgaa agtatatccg ggccggacgc tttgatcata ttccgttaag
     7081 gataaattag gtatctttct cttatcgccc tgaatgccga gagtcctggc tcccaggatt
     7141 cccggatttc atcttctcct tttccgctaa gaatatccag ccttaaccgg tcggtaccgg
     7201 ccagcttatc aaaataggag ttgaagaagt cggccctccc tgagaaatac cggtacgatt
     7261 caatcagcca aaacaaattc agttggttcg ggtggttgag aaagtcgtgg gcgtactcag
     7321 tcacctcgtg ccccaggcat tcctctcctt caaacagtgg tttttcagcc atccccggaa
     7381 tcttcaccgg ggtaaattta taactgccaa ttaaaaaacc ggggtggccg aatacctgga
     7441 aaggccccaa ggtgcctctt ccgacactga ctatggtgcc ttcaaagaag caggttgaag
     7501 gatacaggta aatcgattcc catgtggtca gattgggtga gggcctgaat ggcagggtta
     7561 ccagcatgtt gtgatcatag ccttccatgg ggatcactgt aagttcgcat ttccttgaac
     7621 caagccatcc ttctccattg accatcagcg catattctcc gatagtcatt ccataaacaa
     7681 caggcaccgg atgcatgcca acaaatgatt taaagttcgt gtcgagcacc gggccatcgg
     7741 tataaaatcc attcggattc ggccggtcgg taaccaccac cggcagatcg ttttcagcgg
     7801 cagcctccat gaccagggcc agggtggaaa tgtaggtgta aaatcttacg ccgacatcct
     7861 gtatgtcaaa gataatcaga tcaatacctt ccagatcctg ttgcgctggc tttttattgg
     7921 tcccataaag ggaaacgatc ctgatgcccg ttctttcatc ggtgccgtga tcgatggagc
     7981 cgccagcttc cacgtcgcct cggataccgt gctccggtgc aaatattttt tcgatcttca
     8041 cgccgctttc taaaaggtaa tcaactagat gagttgttcc gatgcgcgag ttctgattgc
     8101 agacaatccc cacccttttt cctttcagta atggaacgta aacatcagac cgttcagctc
     8161 cggttcgaat ggccgtgtat ggtacctgcc ggatcgcagt cgactgcgat ggctgtgcat
     8221 ttcccgtctg aataccggag aatgccatcc ataagatcga aaggactatt aaaatatatt
     8281 gatggatcat catcctgatt gcccttgttt ttcttaaagt tatcatgaaa tgcaatcaaa
     8341 ccgttattcg ttcttatttt taccgattaa ttcgtgaata gttttgaatc tgccctcatt
     8401 catagccaat cgcatcagcg gtaaagataa agaaaatttg tcggggccgg ctgtccgggt
     8461 ctctgtattt accattgctc tcggtctggc tataatgatc ctggctgtgt ccgtccttgt
     8521 gggtttccag aaggagatca gaaacaaagt gattggattc tcagcccaca tccagatcga
     8581 taacttcgac gacaatacct cctttgaatc ccagccgatc cctgctgatc agccatttta
     8641 cgccgaattg aaaggaacgc ctggaataag gcatatccag gtcttcggcc ttaaagccgg
     8701 gatcataaaa acccaggatc agctccaggg cgtgatcctg aaaggggttg gcccggatta
     8761 tgactggaac tttatgaaaa atcatttggc cgaaggggaa ataataaccc tggaagattc
     8821 tgccgcttcc tccggtatcc tgatttcaag gctgatggcc tcaaagctga aaattatacc
     8881 gggagatgaa gtcaggatgt actttgtctc cggctccgat tcacaaccca ggggccggaa
     8941 atttaccgtt gcaggaattt atgaaactgg cctggaagag ttcgatgaaa tgtatgtgat
     9001 agctgatatc cggcatatcc gtcggctgaa taactgggag ccgggaatgg tgtcgggatt
     9061 tgaggttttt attgatgatt ttgataatat tgatgagatg ggagaaaagg tctatcaaac
     9121 aataggttat gacctgaatg cggaaaccat cgtgcagaaa tatccgcaga tattcgactg
     9181 gttaaggctg atggacataa acgtggtcat cattcttgtc ctgatgatcc ttgtagccgg
     9241 aataacgatc atctccacct tgctgatcct tatactggaa aggacaagca tgatcggaat
     9301 tctgaaagcg ttaggtacaa aagaccggaa cctgcgcaaa atgtttattt tcctgaccgg
     9361 ccgtatcatc gccaaaggtt tgttctgggg taacctggcc ggggttggta tcggtttgtt
     9421 acagttttat ttcaggatca ttccgttgga ccaggaatcc tattacatgg cgtatgtgcc
     9481 tgtatccctc atgttatggc agattctgct gataaacctg gtgaccgttg tcacctgcct
     9541 tgtcatggtt ctgattccgg gttacatcat tggcaggatt acaccggtaa aagcgatccg
     9601 ctaccggtaa aaccggttat ttgtgcaaac cgcactcttt catctcagga ttttcccacc
     9661 accaccggcc actgcggata tcatccccgg gttcgacagc gcgggtgcac ggctggcatc
     9721 cgatacttgg aaaccccttg tcgtgcaatg gattatacgg gactttattg atctttatga
     9781 agctttgcac ctgctcgtaa gtccagttga tcagcgggtt gattttaatt ttatgatgaa
     9841 gaggatcgta ttgggcaagc gtaaggtttt cacgggtaac tgactgttct cttctcaggc
     9901 cgctgatcca gaagtcaacg ccggtgaggg cccgctggag cggctcaatt ttccggatat
     9961 ggcagcagag ttttcggttc tcaacgctgt cgtaaaacag gttgatgcct ctgcttgaaa
    10021 ccatctcttc taccttctga ttatcaggaa aataaacttt aatttttaca ttatatctta
    10081 aagatgtcag gtcgataagg tcatacgttt ctgcgaacaa ccgtccggta tccagcgtaa
    10141 atatcgggaa accgggatca atgcgggcaa tcatggcggt cagggcctgg tcttcgaggc
    10201 cgaggctggt ggaaaaagct gcccggtccc tgtgattttc aattatatat gcaataatat
    10261 cttccggcgc cctgtctgcg aaatcacggt tcagtttgtc agcgtaagaa atggcaaatt
    10321 ctttctccat cttgattata acgtttatgc cggtgggcaa aagtaataat aatctatttt
    10381 tgatggataa acggatggtg atcatgaaag ttcatttcat tgcaataggc ggaagcgcta
    10441 tgcacaacct ggcgattgcc ctaaaactca aagggtaccg ggtaaccggt tcggatgatg
    10501 aaatcttcga accggcacac ggtcggctcg gcaaatatgg catcttgcct gagaaggagg
    10561 gatgggaccc agcaaaaata accccggacc tggaagctgt tattctgggc atgcatgcca
    10621 aaggcgacaa cccggaactg gctgctgccc gaacgttggg acttcggatt tattcctatc
    10681 ccgaatatct ttatgaagct tcgaaagaca agatccgcgt ggttattgcc ggtagccatg
    10741 gcaaaaccac gattacctct atgatccttc atgtgctgca aagggtcggg attgagaccg
    10801 actacatggt cggcgcgcag ctggaaggtt ttgaggttat ggtaaagata agtgattcgg
    10861 cccggttcat ggttctggag ggcgatgaat acctttcatc aacgctcgat ccccgcccta
    10921 agttccatct gtaccgtcct gatattgcgc tgcttagcgg aattgcctgg gatcatatga
    10981 atgtctttcc gacttttgaa aattatattt cgcagttcag ccggtttctt gaatgctttg
    11041 aaccgcgcgg aaagctggtt tattgtgaat ccgatccggt ggtggcatcc atttcggaat
    11101 catcccgcaa ggatattgaa aaaattccct atggcaatct tgactatata ataaaggaaa
    11161 agaaaacccg gttgctggtt aatggccttg aatttcccgt gaatgtattc ggccggcata
    11221 acgtgcagaa tattcacggg gcctggctga tatgcaggca gttgggtgtt tccgatgaag
    11281 aattcctgat ggctatcgaa tctttcagcg gagcgtcaaa acgattgcag atcctggccg
    11341 aatcgccgga ttgtaatgtt tacaaggatt ttgcgcacag cccttccaag cttaaagcta
    11401 ctactttggc agtgaaagag ttatttcccg aacggaaact ggtggcctgc ctggagttac
    11461 acacttacag cagccttaac aaagattttt tacctcttta caaaggatgt atggatgctg
    11521 ctgatattcc ggtagtttat ttcagccatc atgcgcttgc actgaaacgt ttgccggaat
    11581 taaccactca agacatccgg gatgcattca gtctggatcg cctgatggtt tttaatgatt
    11641 cccttaaaat gaaggaattt cttctcagcc ttgatttaac aaacaagaat ctgcttttga
    11701 tgagttcagg taatttcgac ggcctggacc ttgacgacct ggctgaaaaa attcttccgg
    11761 tgtgattctt gccggtttgg cgttccttat caggagttca tggagatcag gaactcctcg
    11821 ttattccggg tgtatttcat cttgtctttc aggaactcca tcgcttcgag cggattcatg
    11881 tcggccaggt ggttcctgag gatccagata cgctgaagcg actccttgtc aaccagtaat
    11941 tcttcgcggc gtgtgctgga ggcaacaatg tcgatcgccg ggtagatacg cttgttggag
    12001 agtttccggt cgagctggag ctccatgttt ccggtacctt tgaattcttc aaagataact
    12061 tcgtccattt tcgatcccgt gtcgatcagc gcagttgcga tgatggtcaa cgatccgccg
    12121 ttttcgatct ggcgggctgc tccgaagaac cttttgggct tctggagggc attggattca
    12181 acgccacccg aaagtacttt gcccgaagcg ggagatactg tattatatgc acgggccagg
    12241 cgggtaattg aatcaaggag gatcaccacg tcgtgtccgc attcgaccag ccttttggct
    12301 ttctccagca cgatattggc gatcttggca tgccgttcgg caggctcatc aaaagtagaa
    12361 gcgatgactt ccgctttaac atgccgttgc atatcggtaa cttcctccgg acgctcatcg
    12421 atcagcaata ctataaggta tgcttcaggg tggttgtagg caatggcatt ggctatttcc
    12481 tggagaatga ttgtcttacc tgtcttaggc tgagcgacga tcagacctct ctgcccttta
    12541 ccgatcgggg agaacatatc gataacccgg gtggtgatgc tggcattcgg gtagtcggtg
    12601 attttaaatt ttgtcagggg gaaaagagga gtcaaaaagt cgaacggaat cctgtcgcga
    12661 acttcttccg gtttccttcc gttaatcatc tccactttga tcagcgggaa gtacttttcg
    12721 ccttctttcg gcgggcggat
//
LOCUS       DAOPNH010000060        12738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5090_length_12738_cov_7.654104, whole genome shotgun sequence.
ACCESSION   DAOPNH010000060 DAOPNH010000000
VERSION     DAOPNH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5090_length_12738_cov_7.654104"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKZ74_04735"
     CDS             complement(<1..559)
                     /locus_tag="PKZ74_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPM92097.1"
                     /translation="MLDTGSTGFMLLACSLVMLMTPGLAFFYGGLATKRNILGIMIQS
                     FVSLGWTTVLWFIFGYSLCFSGGEGGIIGNLDKAFLNGVGIDSMYSNGKIPELVFVAY
                     QMMFAIITPALITGAFVNRVTFKAYLIFLTVWQILVYYPFVHMVWGGGLLAQMGVLDF
                     AGGIVVHATAGFAALASVFYIGARVD"
     gene            complement(609..1367)
                     /locus_tag="PKZ74_04740"
     CDS             complement(609..1367)
                     /locus_tag="PKZ74_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92098.1"
                     /translation="MKGSKKYWIILFAILAIPFRPLTAQEEKKLQPELGCDIVSSYIW
                     RGSAVYATLGDQNVLSPSLQPGFALGFQGLKLGAWGSVDFTGTYKELDYYLSYSLKGF
                     TLSVTDYYWASDWINDNFFEYGSDSTSHYLEASLAYKGEKIPVSILVATMLYGADKQF
                     DDPAKNNYSTYIEVGYGFKVSDYKLDLFLGMTPSDGMYGDGYGGRDGFAIVNAGVTGS
                     KTIKITENWSLPVKASLIANPQARKLFLAFILSI"
     gene            complement(1460..2110)
                     /locus_tag="PKZ74_04745"
     CDS             complement(1460..2110)
                     /locus_tag="PKZ74_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92099.1"
                     /translation="MQKIKIILVDDHQIIRDGIRALFNGCTDIEIIAEAADAAECLQQ
                     LRLRQADVALIDISLPGISGIKLTEQLSVLYPSLKVLILSMHLNEAYISGAIKAGAMG
                     YLAKNTTREELISTIHLIAEGKKYLGKDVSEVITTGFVRRLQANENENTELLSKREME
                     ILKLTADGLGNREISQKLFISIRTVESHKNHIMQKLGLKSSVELVRYAIKSVLINI"
     gene            complement(2122..4272)
                     /locus_tag="PKZ74_04750"
     CDS             complement(2122..4272)
                     /locus_tag="PKZ74_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPM92100.1"
                     /translation="MKKISIRERLILYFVLLGVISIAIVSLFSIFESKRGIRERAFSQ
                     LILLRDIRREQILNFYQVRKTELATLARTPVIRNLADRLEELQESGNFPVGLENDREF
                     LNLVLDNHICKVLYLVSVENNITKISGTDDIFLINKDEDLKVNESIMRLFEGSVTPDT
                     FHMTEWIGESNRPCLILTVPVFSSAGEVSALLLSRIEPEALNNIIFDVVPGTGMGKTG
                     EAYIAGPDGFMRTPSRFLPDAVMTVRINTEGFNKALTGIDSTGIYDDYRGIKVLGAYG
                     KIIFGGFTQLILAEIDVDEAMVPLAIIRNDILIVSSLILLGIFFITWFVAYGITRPII
                     RLKNAANVIASGSYNQKLKVGPEDEIGELTEAFNRMSEEVSIATRELREKEESLRHFY
                     DATLDGIVLHNDGKMVLFNAAILRLTGYTENELAALVIHDILKETKSLYCDKNQDNEI
                     YETLLFRKDNSSLPVEVQESCVEYKGNKIRASVIRDISTRKSMETELADERMKRLRAV
                     IDGKELEQQRLSRELHDGLGQQLAAGKLILESGLYTEGEKLKGKIVEAQAIFDQIIGD
                     IRRISHDLSPSVLKEFGLKEAVENLCASFRMTTGLRISFFCESDGYQPDEMKAIYLYR
                     IIQECLNNIQRHAGATLVSVFLKAGPQGIQLEIKDNGCGFNQKNVPASGGTGLYNIRE
                     RVNVMNGQLNIMTGKNKGTVIRIRIPLTDGGQNE"
     gene            4416..7211
                     /locus_tag="PKZ74_04755"
     CDS             4416..7211
                     /locus_tag="PKZ74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM92101.1"
                     /translation="MKRSLFLLIFAGLFFAGTNPEEKQVKIEYEKYKLANGLDVILHI
                     DRSDPIVALAVQYHVGSNRETEGRTGFAHLFEHMMFQRSENVPEDQFFKLIQDAGGTL
                     NGGTSNDATTYFEVVPKNALEKILWMESDRMGFMINTVTKKSFAIQQNVVQNEKRQNY
                     DNRPYGFTQEVVAKNLYPEGHPYNWTVIGEMEDLFNASVEDVKAFHQRFYLPNNATVV
                     LAGDFDVDEAKKLIEKYFGEIPAGNEVKDPVPVPVKLDKTKKLYHEDNFAKAAQLRMI
                     WPTVEQFSDDSYALSYLGQLLSQGKKAPLYKVLEKEKKLTTSQNAYNSSQEIAGSFSI
                     NVTANDGVSLKDVEEAVFEAFRMFETEGFTDEDVERIKAGQETQFYNGISSVLGKSFQ
                     LAYYNEYAGDPSYYKTDIGKTKAVTRQDILRVYEKYIKNQPYLATSFVPKGQLSLVAE
                     GSADAGVKEESISEATQVTIEEMEEVEIAMTPTSFDRTVIPVDGPDPSVTLPEIWDTK
                     LDNGMRVLGIENTELPLVQFNIVLKGGHYLDKMEKSGTALLLSELMMEGTKNKTPLEL
                     EEEIEKLGASINISASAIDITISANTLARNYDKTLALVEEILLEPRWDAEEFELAKSR
                     LQNRLLRSKADPNALARDAFNKLVYGPDHIFSLDRQGNETTVPGIAIEDLKAFYELNF
                     SPTVASFHIAGDISREKVMTSLRKLEKEWIAKDVEFPVYELPEPLPKSKIFFVDVPGA
                     KQSVISIGCMGLSRTDEDFYPANVMNMKLGGSFSGNVNLVLREEKGFTYGARTGFSGT
                     YIPGTFQATASVRSSATVESVEIFKRLMEEYRNGISEEDLIFTKNSINKSRARDFETL
                     GAKNRMLQEISMYNQPLDYVRRQEQIVSDMTREEHKALAEKYIVPDRMYYVIAGDAAT
                     QMEALGQIGFGAPEAVK"
     gene            complement(7214..7825)
                     /locus_tag="PKZ74_04760"
     CDS             complement(7214..7825)
                     /locus_tag="PKZ74_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92102.1"
                     /translation="MRTNILILIIFSLTVLAGVENASAQWAVNGSHIYNTNAGNVGIG
                     NNAPASLLYVGKSMTEPTITVRNLGGAGGATYSMVDDASGANWKFKATNSGGFKIRDF
                     ANSLDVFQIEANSAANVIYINAAGAIGINTATPATSALMELNATDRGFLPPRMSESQL
                     SAINNPANGLMAFNTTDGKLYVFVESVNSWKEVSFGAGTIVPQ"
     gene            complement(7865..9076)
                     /locus_tag="PKZ74_04765"
     CDS             complement(7865..9076)
                     /locus_tag="PKZ74_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92103.1"
                     /translation="MKTKYISLFVLLLLTINFIYSQWAPNGSHIYNTNAGNVGIGNNA
                     PASLLYVGKNMTEPTITVRNLGGSGGATYSMVDDASGANWKFKATNSGGFKIRDFANG
                     LDVFQIEANSAANMMYINAAGSVGIGTAAPASSALVDMSSTTQGFLLPRMTQTQISLI
                     QNPANGLLVFNLTDNKVYAYVAGAEAWKELSFGTEILTPPFICGDPITVNHVAGAVAP
                     VNKTVTYSTVTNVPGATTKCWITSNLGSDHQATAVNDATEASAGWYWQFNRMQGYKHD
                     GSTRTPNTTWIQGIDESGDWQTAQNPCTIELGAGWRIPTWTEWENVKTSGGWTNWNGP
                     WGSVLKMHAAGKLLQASGGLDSRGIQGVYWSSTQGSYTVSAINMTFISTSIGVAYGDK
                     AYGETIRCIKD"
     gene            complement(9099..10310)
                     /locus_tag="PKZ74_04770"
     CDS             complement(9099..10310)
                     /locus_tag="PKZ74_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92104.1"
                     /translation="MKMKILLMGIFLLLIFNFPLVVKSQWAVNGSHIYNTNAGNVGIG
                     NNAPASLLYVGKSMTEPTITVRNLGGSGGATYSMVDNVSGANWKFKATNSGGFKIRDN
                     AFGLDVFQIEANAAANVIYISAAGSLGVGTATPEATAIADLSSTSRGFLPPRMTQAQL
                     SAIPAPANGLLVFNTTDEKFYIYVELVNAWKEILYGTGTISTTYVCGTPITRNHVAGS
                     VAPVSKTVTYNSVTNIPGETTKCWITQNLGSDHQATAVNDATEASAGWYWQFNRQQGY
                     KHDGTTVTPAWTITSISENSDWTSDHDPCVFLLGTGWRIPTSTEWSNIDAAGGWTTWN
                     GPWNSDLKMHAAGHIGGSTGTLVYRGQYAGYWGSTQTGNTTAYRLIFGSAECELYNDD
                     KANAFSVRCLR"
     gene            10692..12410
                     /locus_tag="PKZ74_04775"
     CDS             10692..12410
                     /locus_tag="PKZ74_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92105.1"
                     /translation="MLAYSGYNKPIGIAACVAFIIAVILPEFLLSQDTITTQGEKLFS
                     IHGNISLNLIGYYSRGIPSRSVPFSAILSASATPKVKNFELPFSLVFSGNEIGLTHTS
                     QSFSQIGISPRWKWITLHGGYRNVTFSNFTLAGHTFLGGGIELIPGKFRFGFVCGRFD
                     RKTTGSNASTTDKLSHFARRGFAVKLGIGTEKNFFDLLFLRIRDDSSRLQQPDTGTIT
                     LPEQNIVAGFNGHYTIARKLTWDMEGAFSLYTTNLAAQPIEEIEEDNTLHSLNKFLGI
                     NFSSEYYYAIRSSLQYKERNWGVKLDYRRIEPGYRSMGAYFFNNDVQNITLSPSFALF
                     KRKLTVSGAIGLQEDNLRGTKRATSARTIGNVNISFNPKPKYGFTASYSNYSINQRAG
                     TIPLNDTTRVRQTTHSFMFTPRFMLITPERSHMVMLVYNLVAGRDKNEFTAPFTRFTS
                     QIAQMTYTLGLTKTKWSVTAGVTFSLVSNFIADISWIGGTTGISKTLMKDKLSLGWNN
                     AVTRKNAGSTDAWIYNSSLTSTYRAGKHHNFRLYIYFTGNFTGTGSYNPSFNELKGDL
                     SYVFTF"
     gene            12394..>12738
                     /locus_tag="PKZ74_04780"
     CDS             12394..>12738
                     /locus_tag="PKZ74_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92106.1"
                     /translation="MCLLFRKRIRWLFRSLLLTGPLSLIWLIYSVPVFSQALKQEFPV
                     HISVVVSPPYSKNIDTYISQPDKVMATFLSNAPAPVDVYILGVFEGENGIRVYTDPGY
                     KMYPPVTLLPGVP"
BASE COUNT         3229 a         3193 c         3059 g         3257 t
ORIGIN      
        1 tatcgaccct ggcgccgatg tagaataccg aagcgagggc tgcaaaaccg gctgtggcat
       61 gaacgacaat cccgcccgcg aaatcaagca cacccatctg ggccagcaaa cctccgcccc
      121 aaaccatatg gacaaaagga taatagacca atatttgcca gaccgtcaga aaaatcaggt
      181 aagctttgaa agtaaccctg tttacgaaag ctcctgtaat taaagccggt gtgatgatcg
      241 caaacatcat ctggtacgcc acgaaaacca gttcgggaat cttcccgttg gaatacatgg
      301 agtctatgcc caccccgttt aagaatgctt tatccaggtt cccgattatt ccgccttctc
      361 ctccgctaaa gcaaagggag tagccgaaaa taaaccagag aacagtcgtc catcctaaag
      421 aaacaaagct ctggatcatg atcccgagga tattcctttt tgttgcaagg cctccgtaaa
      481 aaaatgcaag gccgggggtc atcagcatga ccaggctgca ggccagcagc atgaacccgg
      541 ttgaaccagt atctaacata attttaaatt ttgaattatt aattttgaat tataaattaa
      601 tgccttgatt atatagataa tatgaaggcc aagaataatt ttcgagcctg tggatttgca
      661 attaacgagg ctttgaccgg cagtgaccag ttttcggtga tcttaatcgt ttttgatccg
      721 gttaccccag cattaacaat ggcaaagcca tccctgccgc cgtatccgtc gccgtacatg
      781 ccgtcggacg gggtcatccc caaaaacagg tccagtttat aatcggatac cttaaagcca
      841 tatccgactt cgatatacgt tgaataatta tttttcgccg ggtcgtcgaa ctgcttatcg
      901 gccccatata acatggttgc caccagaatg ctgacgggaa tcttttcgcc cttgtaagcc
      961 agcgatgcct ccaggtagtg tgaagtgctg tcgctgccat attcgaagaa attatcgttg
     1021 atccagtctg aagcccagta ataatcggtc accgacaggg taaatccttt caaagaataa
     1081 ctcaggtagt aatccagctc tttgtaagta cctgtaaaat cgaccgatcc ccaggccccg
     1141 agtttcaatc cctggaagcc caaggcaaat cccggctgta aactcgggga aagcacattt
     1201 tgatcgccta gtgttgcata tacggcagaa ccccgccaga tataactcga cacgatatca
     1261 caacccagct cgggttgtag ctttttttct tcctgggctg tcaaaggccg gaagggtatc
     1321 gccaggatgg cgaacagaat aatccaatac ttttttgaac ctttcatagt tgtttttttt
     1381 aagaaaggtc aaattaaaga attgaagatc aaccgggaat cagaattatc accgaaatct
     1441 ttaagtaaaa cactgaattt taaatattga tgagcactga tttaatcgca tacctgacga
     1501 gctcgaccga tgatttcagc cccagtttct gcatgatatg gttcttatgc gattcaacgg
     1561 tccttatact gatgaataat ttttgagaga tttccctgtt tcccaatcca tctgcggtca
     1621 attttaagat ttccatttct ctttttgaaa gcagttcagt attttcattt tcattggcct
     1681 ggagtcgccg gacaaaaccg gtggtgatga cttccgaaac atctttgccc aggtattttt
     1741 tgccttcggc gatgagatga atggtggaga tgagctcttc acgcgtagtg tttttggcca
     1801 gataccccat ggcgccggct tttatggcgc ctgaaatgta agcctcattc aggtgcatgg
     1861 acaatataag gacttttaat gatgggtaca ataccgaaag ctgctcggtg agttttattc
     1921 ctgaaattcc cggcagactg atgtcgatga gagccacatc cgcctgccgt agcctgagtt
     1981 gctgcaaaca ctcagcggca tcagcggctt ccgctataat ttcgatatcc gtgcatccgt
     2041 tgaataaagc ccggattcca tcccggatga tctggtgatc atcaacaaga ataattttaa
     2101 ttttctgcat aagaatttat gttattcatt ttgtccaccg tcggtcaatg gaatcctgat
     2161 gcggatcacc gttcctttat tttttccggt catgatattt aactgaccat tcatgacgtt
     2221 gaccctctcc cggatattgt acagcccggt tcctcccgac gccggcacgt tcttctgatt
     2281 aaagccgcat ccgttatcct ttatttcaag ctgaataccc tgcggaccgg ccttaaggaa
     2341 gactgataca agcgtagccc ctgcatggcg ctgtatgttg ttcaggcatt cctggatgat
     2401 cctgtaaaga tatatggcct tcatttcatc cggttgataa ccatcggatt cacaaaaaaa
     2461 actgatccgc aaaccggtgg tcatccggaa actggcacag agattttcca ccgcctcttt
     2521 cagaccgaat tctttcagga cggaagggct gaggtcgtgc gatatgcgcc ttatatcgcc
     2581 gatgatctgg tcaaagatgg cctgggcttc cacgattttc cctttcagct tttccccttc
     2641 ggtgtaaagt ccgctttcca gtatcagctt cccggccgcc agttgctggc ccagcccgtc
     2701 gtgcaattcc ctggaaaggc gctgctgctc cagctctttg ccgtcaatta ccgctctgag
     2761 ccttttcatc cgttcatcgg ccagttcggt ctccatgctt tttctcgtgc tgatgtccct
     2821 gatcacgctc gccctgatct tgtttccctt gtattcgaca caggattcct gaacttcaac
     2881 cggcaggctt gaattgtctt tcctgaacaa cagtgtttca taaatttcat tgtcctggtt
     2941 cttatcgcaa tataatgatt tggtctcctt cagaatatcg tgaatcacca atgccgccag
     3001 ctcattttcg gtgtatccgg tcaatcgcag gatcgctgca ttgaagagga ccatctttcc
     3061 atcattatgc aacacaatac catcgagggt agcatcataa aagtggcgaa ggctttcttc
     3121 tttttcccgt aattcccttg tcgcgatact gacctcttcc gacatcctat tgaaagcttc
     3181 ggtcagttcc ccgatctcat cttccgggcc gacctttaat ttctgattat aacttcctga
     3241 tgctattaca tttgcggcat tttttaaacg aatgatcggc cgggttatac cataagcgac
     3301 aaaccaggta ataaaaaaga tgcccagaag gatcagggaa gagacgatca aaatatcatt
     3361 ccgaatgatc gccaatggca ccatggcttc atctacatca atctcggcaa ggatcaattg
     3421 ggtgaatcct ccgaaaataa tctttccata ggctcctaaa acctttattc ccctgtaatc
     3481 atcataaata cctgtactgt caatcccggt taaagccttg ttgaatcctt cggtattaat
     3541 cctgacggtc atgacggcat ctggtaaaaa gcgggaaggg gttcgcataa aaccgtcagg
     3601 gcctgcaata taagcctccc cggttttgcc catccccgtt cccggaacca catcgaaaat
     3661 gatgttattt aaggcttcag gttcaatccg ggataataac aacgcggata cctccccggc
     3721 tgaactgaat accggaaccg tgaggatcaa acagggacga ttagattctc caatccattc
     3781 tgtcatgtga aaagtatccg gtgtgaccga tccttcaaat aaccgcatta tactctcatt
     3841 gactttgaga tcttcatcct tattaatcag gaatatatca tcagttccgg atatttttgt
     3901 gatgttgttt tcaactgaaa ccaaatacaa aactttgcaa atatgattat ccagcacgag
     3961 gttaagaaac tcacggtcat tctctaaacc aacaggaaag tttccgcttt cttgcaattc
     4021 ttccaaccgg tctgcgagat tcctgataac gggtgtccgg gccagtgtag ccagttcggt
     4081 tttgcggacc tggtaaaaat ttagaatttg ttctctgcgg atatccctta gaaggatcaa
     4141 ctggctgaat gccctttccc tgatccctct tttggattca aaaatcgaga aaagacttac
     4201 aatagcgatg gatatgacac ccagaaggac aaaataaagt atcagccgtt ctctaatgga
     4261 aatcttcttc atggatcaca aagaaacaaa atagtatggc tggcatgtta aataaccgtt
     4321 tataatgatg tttttgcaat ttaacttttg tcatttgcaa attctcctat gcagttgtta
     4381 tgcaattttt ttagttttat caaaaatttt ttaaaatgaa aagatcatta tttctgctaa
     4441 tctttgccgg actctttttt gccggtacta atcccgaaga aaaacaagtg aaaatcgaat
     4501 acgaaaaata caagctggcc aacgggctcg atgtgatcct tcatatcgac cggtccgatc
     4561 ctattgtcgc actggccgtt cagtaccatg tcggttccaa ccgggaaacg gaaggccgca
     4621 ccggctttgc ccacctgttt gaacatatga tgttccagcg ctcggagaat gtccccgaag
     4681 accagttctt caaactgatc caggatgcag gcggaacgct gaacggcggc acctctaacg
     4741 acgccacgac ctactttgag gtggtcccga aaaatgccct ggagaagatc ctctggatgg
     4801 aatccgaccg gatgggattc atgatcaata cggtgacgaa aaaatctttt gccatccagc
     4861 agaatgtcgt ccagaatgaa aaacggcaga attacgacaa ccgcccatac ggctttaccc
     4921 aggaggtggt ggccaaaaac ctttacccgg aagggcatcc gtacaactgg accgtcatcg
     4981 gagaaatgga agatcttttc aacgcaagtg tagaagatgt gaaagctttt catcagagat
     5041 tctatttgcc gaacaacgct acggtggtcc tggcaggaga ttttgatgtg gatgaagcga
     5101 aaaaactgat tgaaaagtac ttcggggaga tccctgccgg caatgaggtg aaagacccgg
     5161 tcccggttcc ggtaaagctg gataagacga aaaagctgta tcatgaagac aactttgcca
     5221 aagcggcgca gctccggatg atctggccaa ccgttgagca gttttccgac gactcttatg
     5281 ccctctctta cctgggccag cttctttcgc aagggaaaaa agccccgttg tacaaagtcc
     5341 ttgagaaaga gaaaaagctt actaccagcc agaatgctta taacagcagc caggagatcg
     5401 cggggagctt cagcatcaat gtgactgcca acgacggcgt cagcctgaag gatgtcgaag
     5461 aggccgtttt tgaggcgttc cggatgttcg agaccgaagg gtttaccgat gaggatgtgg
     5521 agaggatcaa ggcgggccag gaaacccagt tctacaacgg gatcagcagc gtgctgggca
     5581 aatcgttcca gctcgcatac tacaacgaat atgcaggcga tccgtcgtat tacaagactg
     5641 atatcgggaa gaccaaagcg gtcacccggc aggatatcct aagagtatat gagaaataca
     5701 tcaagaacca gccctacctg gccacgagct ttgttccgaa aggtcagcta tcattggtgg
     5761 ctgaaggttc ggcagatgcg ggggtgaaag aagagagcat cagcgaggcc acgcaggtga
     5821 ccattgagga aatggaagaa gtggagattg ccatgacccc gacctctttc gaccgcacgg
     5881 tgatcccggt tgacggcccc gatccgtcgg tcacgctgcc cgaaatatgg gacacgaagc
     5941 tcgacaacgg gatgcgggtg ctgggcattg aaaataccga gctgccgctg gtccagttca
     6001 acatcgtttt aaagggaggc cattatctcg ataagatgga aaaatcgggt acagccctgt
     6061 tgctatcgga gctgatgatg gaagggacga agaacaagac cccgctggag ctggaagagg
     6121 agatagagaa gctgggcgcc agcatcaaca tcagcgcttc ggccatcgat atcaccatca
     6181 gcgccaatac cctggcaaga aattacgata agacgctggc ccttgtggaa gagatcctgc
     6241 tggaaccgag gtgggacgct gaggagtttg aactggcaaa atcgaggttg cagaaccgcc
     6301 ttctccggtc caaagcagat ccgaacgccc tggcccgtga tgctttcaac aagctggtct
     6361 acggcccgga tcatattttc tcgctcgacc gccaggggaa tgaaaccacg gtgcccggaa
     6421 ttgcgatcga agacttaaaa gctttctacg aactcaattt ttcgcccact gtggcctctt
     6481 tccatatcgc aggcgacatc agccgcgaga aagtcatgac atcattaaga aagctggaaa
     6541 aagaatggat tgcgaaggat gttgaatttc cggtatatga acttccagag ccacttccga
     6601 agtcaaaaat ctttttcgtc gacgttcccg gggccaagca gtcggtcatt tccatcggtt
     6661 gcatgggcct gtcgcggacc gatgaggatt tctaccccgc caacgtgatg aacatgaagc
     6721 tgggcgggtc gttcagcggc aacgtcaacc tggtactgcg cgaagagaaa ggatttacct
     6781 acggcgccag gaccggtttc tcggggacgt atattcccgg caccttccag gcgacggcca
     6841 gcgtaaggtc gtcggcaacg gtggaatcgg tggagatttt caaacggctg atggaagagt
     6901 accgcaacgg gatcagcgaa gaagacctca tcttcaccaa aaactcgatc aacaaatcga
     6961 gagcacggga ttttgagacc ctgggggcca aaaaccggat gctgcaggag atcagcatgt
     7021 acaaccagcc gctggactac gtccgccggc aggagcagat cgtaagcgat atgacccgtg
     7081 aggagcacaa agcgctggct gaaaaatata tcgtccccga ccggatgtac tatgttattg
     7141 ccggcgatgc ggcgacacag atggaggcac tgggccagat agggttcggc gcgccggaag
     7201 cggtgaagta attttattgc ggaacaatcg ttcctgcccc aaatgatacc tccttccagg
     7261 aattcaccga ttcgacaaaa acataaagct ttccgtcggt ggtgttgaat gccatcaagc
     7321 cgttggcggg attgttgatg gccgaaagct gtgattcgct catccgggga gggagaaagc
     7381 cgcggtcggt ggcgttcagt tccatcagcg ccgaggtggc cggtgtggcg gtgttgatgc
     7441 ctattgcccc ggcggcgttg atatagatca cattggctgc gctgttggcc tcgatctgga
     7501 aaacatccag cgagttggcg aagtccctga tcttgaaacc gcccgagttg gtggctttga
     7561 acttccagtt ggccccgctg gcatcgtcga ccatcgaata ggtggcgccg ccagctccgc
     7621 ccaggttgcg gacggtgatc gtcggctcgg tcatgctttt gccgacatag agcagtgaag
     7681 ccggggcatt gttcccgatc cccacattac cggcgttggt gttgtaaatg tgacttccat
     7741 tgacggccca ctgggcactg gcattttcaa ctccggctaa taccgtaagt gaaaatatta
     7801 tcagtattaa tatattggtt ctcatcttaa tatgacgtta caattagaat ttaacatgtc
     7861 tcttttaatc tttgatgcac ctgatggttt cgccataggc tttatcaccg tatgctacgc
     7921 ctatactggt ggagatgaag gtcatattga tggcgcttac ggtatatgag ccctgagtac
     7981 tgctccagta tacgccctga atacctctgg aatccagccc accggaagct tgcagcagtt
     8041 tgcctgcagc atgcattttt agtacggatc cccacgggcc attccaattg gtccatccac
     8101 cggaggtctt cacattttcc cattccgtcc aggttggaat gcgccagccg gctcccagtt
     8161 caatagtaca tggattttga gctgtctgcc aatcaccgct ctcatcaatt ccctgtatcc
     8221 aggtagtgtt tggcgtacgg gtgctgccgt catgcttata cccctgcatc cggttgaatt
     8281 gccagtacca tcccgcagag gcttcagtcg catcattcac tgccgttgcc tggtgatcgg
     8341 agccaaggtt gctcgtaatc cagcatttgg ttgttgcgcc aggtacgttg gtcactgtgg
     8401 aataggtgac ggtcttattc accggcgcca cggccccggc cacatggttc accgtgatcg
     8461 gatcgccgca gatgaacggc ggtgtaagta tctccgtccc gaaggaaagc tctttccacg
     8521 cctccgcgcc ggccacatag gcgtatacct tgttgtcggt aaggttgaag accaacagcc
     8581 cgttggccgg attctggatc agtgagatct gggtctgggt catccgcggg aggaggaatc
     8641 cctgggtagt ggaactcatg tcgaccagcg ctgagcttgc cggggcagcc gttccgatcc
     8701 ccacactgcc cgccgcattg atatacatca tattggctgc gctgttggcc tcgatctgga
     8761 agacatccag cccgttggcg aagtccctga tcttgaatcc gcccgagttg gtggctttga
     8821 atttccagtt ggccccgctg gcgtcgtcga ccatcgaata ggtggcgccg cccgatccgc
     8881 ccaggttgcg gacggtgatc gtcggctcgg tcatattttt cccgacataa agcagagagg
     8941 ccggggcgtt gttcccgatc cccacgttgc cggcgttggt gttgtagatg tggctgccgt
     9001 tgggagccca ctggctgtag atgaaattta tcgtcaataa taacaacacg aataatgaga
     9061 tatattttgt tttcatcatt ttcaatttta tattcatttt accttaaaca cctcactgag
     9121 aaggcatttg ccttatcatc gttgtacaac tcacactccg cgctgccgaa tattaaccgg
     9181 taagcagttg tattacctgt ttgggtgctt ccccagtaac ctgcgtattg gccccggtag
     9241 actaaagtac cagtgctgcc accgatatgc ccggctgcgt gcatctttaa atcagaattc
     9301 caggggccgt tccacgtcgt ccaaccaccg gctgcatcga tattgctcca ctcggtggag
     9361 gtagggattc gccaccctgt ccccagcagg aacacacacg gatcatgatc ggaggtccaa
     9421 tccgagtttt cgctgatact ggtgatggtc caggcaggag tgacggttgt cccgtcatgt
     9481 ttgtatccct gctggcggtt gaattgccag taccagccgg ccgaggcttc cgtagcatca
     9541 ttcactgccg tggcctggtg gtccgagcca aggttttgcg tgatccagca tttggtggtc
     9601 tctcccggaa tattggtcac gctgttgtag gtcaccgttt tgctcaccgg agcaacactg
     9661 cctgccacat ggttcctggt gatgggtgtc ccgcacacat acgtcgtgct gatggttccc
     9721 gttccgtaaa ggatctcttt ccaggcgttc accagctcca catagatata aaatttctca
     9781 tccgtggtgt tgaagaccag caggccattc gccggggcgg ggatggccga caactgggct
     9841 tgcgtcatcc gcgggggtag aaatccccgg gaagtcgagg aaaggtcggc gatggcggtg
     9901 gcttcgggtg tggccgtgcc gacacccagg cttcccgccg cactgatgta gatcacattg
     9961 gccgcggcgt tggcttcgat ctggaacacg tcgagcccga aggcattgtc acggatctta
    10021 aaaccgcccg aattagtagc tttgaatttc cagtttgcac cggaaacatt gtcgaccatc
    10081 gaataggtag cgccgcccga tccgccgagg tttcggacgg tgatcgtcgg ctcggtcatg
    10141 cttttgccga catagagcag tgaagccggg gcattgttcc cgatccccac gttgccggcg
    10201 ttggtgttgt agatgtggct gccgttgacg gcccattggc ttttaacaac caatggaaaa
    10261 ttaaaaatga gcaataaaaa aatgcccatc aacaaaattt tcattttcat ggattccagg
    10321 tttaacggtc aataaatgtt ctgtcaaatt attatttgtc agacattagc gagccagtca
    10381 ataattagaa aagaaagaaa ccggcaaaga tcagtcagta gtttggcagc aaaggctgtt
    10441 aatgaatcaa attgatgcga aagataaata ataatcgggt aaaagtcaat acttaactgc
    10501 ccaaatttta aaatttttca tttgcataag gacttttttt aagtatattt atacccgtta
    10561 acaaaatata ttaactatcc ggggccgcat tcttccaaac actgccatag agcttttctg
    10621 atcggataat aacccgattg ttgccagtta gaataacctc atagctaatc ggtctgataa
    10681 attttatcag tatgttagct tattcaggat acaataaacc tatcggtatt gcagcatgtg
    10741 ttgcttttat cattgcggtc atcctacccg agtttttatt atcgcaggat acaataacca
    10801 cgcaaggtga aaagctcttt tccatccatg gcaacatcag cctgaatctc atcggttatt
    10861 acagtcgtgg cataccttcc cgctccgttc cgttttcggc aattctttcc gcaagcgcaa
    10921 cgcctaaggt caaaaatttc gagcttcctt tttcgcttgt tttttcggga aatgaaatcg
    10981 ggctgacgca tacttctcaa tcattcagtc agataggcat tagcccgcga tggaaatgga
    11041 tcactttgca cggcggttac cggaatgtga ccttctccaa ttttactctt gccggccaca
    11101 cttttctcgg cggagggatt gagctgatcc cgggcaaatt caggtttgga ttcgtctgcg
    11161 gccgcttcga ccggaaaaca acaggcagca acgccagcac cacagataag ctttcccatt
    11221 ttgcccgccg gggatttgcc gtaaagttgg gtatcgggac cgagaaaaac tttttcgatc
    11281 tcctgttttt gcgcatccgg gatgacagca gcagacttca acagcccgat accgggacaa
    11341 tcacattgcc ggagcagaac atagtagctg ggttcaacgg ccattacacc attgccagga
    11401 agctcacctg ggacatggaa ggggctttca gcctttacac aacgaacctg gcggcacagc
    11461 ccatcgaaga gatcgaagag gataataccc tgcattcgct gaataaattc ctgggcatca
    11521 atttctcgtc tgaatattat tatgcgatac ggtcatcgtt acaatacaag gaaagaaact
    11581 ggggtgtaaa gctggattat cggcggatcg agccgggata ccgctcaatg ggggcttatt
    11641 tcttcaacaa cgatgtccag aatatcaccc tcagcccttc cttcgccctt tttaaaagaa
    11701 aactgactgt cagcggcgct atagggctcc aggaagataa cctgaggggg acaaagcggg
    11761 ccacttccgc caggaccatc ggaaacgtca acatttcatt caatccaaaa cccaaatacg
    11821 gtttcacggc cagttacagc aattacagca taaatcagcg tgccggtact attcccctga
    11881 acgatacgac cagggtcagg cagaccacgc acagtttcat gtttactcca cgctttatgc
    11941 tgataacgcc ggaaaggagc catatggtca tgctggttta caacctggtg gcaggccgcg
    12001 ataaaaatga gttcaccgct cccttcaccc ggtttacatc ccagattgcc cagatgacct
    12061 ataccctggg cctgacaaaa acaaaatggt cggtcacagc cggggtcact ttttcactgg
    12121 tcagtaactt cattgcagat atcagctgga tcgggggaac gacaggcata tcgaaaacat
    12181 taatgaagga taagctgtcg ctgggctgga ataatgctgt aacccgtaaa aacgccggat
    12241 caactgacgc ctggatttac aattcaagcc ttacgtccac ctatagggca ggcaagcacc
    12301 acaacttcag gctctatata tatttcaccg gcaatttcac cggtacaggt tcatataatc
    12361 catcattcaa cgaactgaaa ggagatttga gttatgtgtt tactttttag gaagaggatc
    12421 cgttggctct ttcgcagcct tttgctgacc ggtccgctga gtttaatctg gctgatatat
    12481 tccgttccgg tttttagcca ggcccttaaa caggaattcc ctgtccacat ttccgtggtg
    12541 gtttcgccgc cttattccaa aaatatcgac acttacatct cccagcccga caaggtgatg
    12601 gcaacatttt taagcaatgc cccggcgcct gtagatgttt atattctcgg tgtttttgag
    12661 ggggagaacg gcatcagggt ctataccgat ccgggatata aaatgtaccc cccggttacc
    12721 ctgttgcccg gggtaccg
//
LOCUS       DAOPNH010000061        12557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5213_length_12557_cov_6.214766, whole genome shotgun sequence.
ACCESSION   DAOPNH010000061 DAOPNH010000000
VERSION     DAOPNH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_12557_cov_6.214766"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2928
                     /locus_tag="PKZ74_04785"
     CDS             <1..2928
                     /locus_tag="PKZ74_04785"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPM92107.1"
                     /translation="KLQVGHPPGAPTFQLIGRFFSLFAFGNTANVAMMINTMSAMVSA
                     LTIMFLFWTITLLARKMVKADAGYTLTQVILLFGSAFVGAMAFTFSDSFWFSASEGEV
                     YAMSSLFTAITFWAILKWEVVADEKHSLRWLILIAFLIGLSIGIHLLNLLAIPAITFV
                     YYYRKYKPSRKGLLLAGVVSVLILALLMYFFIPGIVKLAGSFELFFVNSIGLPFNSGT
                     IIYFALIIGLIVWGLKYTSKKGKSVLNSIILAFVFLLIGYSSFIILIIRSNANTPIDE
                     NNPEDAISLLSYLNREQYGTYPLYYGQYYNAPVVDYKDGTPVYLKDRSKGKYVITDDR
                     KESLPVYDSRFTTLFPRMWSDQKSSHINKYKEYGKIKGTPITVSKPDGTTETLYKPTF
                     VENMRFFFSYQLGHMYFRYFLWNFAGRQNDIESQGEPQNGNWISGFNFLDNGRLGNQS
                     DLPESMQNTARNKFYLLPLLLGIAGILYHFKKNRNDTWVVFLLFLMTGIAIVVFLNQT
                     PYQPRERDYAYVGSFYAFAIWIGLGVMGLAEFIQKYVRKQEVAVAAAAVGLSFLLVPG
                     IMAHQGWDDHDRSGKYAARDFAMNYLRGCDKNSVLVTFGDNDTFPLWYVQEVEGFRTD
                     VRVVNHMLASGHWYVQQLFSKVYDSDPLPFTLRIDQYNNGVNNYVPVYEIPSLANEYT
                     ELSELIRFVALDSDKSKIPISSTKKINFFPSRKVKMTVDAKKCVENGIVPPELADRIV
                     PVIEWEIKQNALYKNDLAVLDFLASSNWERAFYTANPSSLLDFLDIDQYLHQEGLVYK
                     FMPVKADNYYRGVGGVNGMKTYENFMNCRWGNLNQPNILVDRESYRNSRLPRQNYLRA
                     AETLLINGDKQKAVELLDRCNEFFPDNKINYDMIMIPFAEVYYGAGEPEKGNEIILKL
                     LDYYADDLRYYNTVKPSFVEKYYSDSITRIMRILREIAQVTRTNGQPEISAKATELME
                     SYK"
     gene            complement(2929..3744)
                     /gene="lgt"
                     /locus_tag="PKZ74_04790"
     CDS             complement(2929..3744)
                     /gene="lgt"
                     /locus_tag="PKZ74_04790"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPM92108.1"
                     /translation="MDLLSITWSPDPEIFRIGTFAVRWYGVLFALGFVCGYFIILGFF
                     RREKIPVKLLDQLTTYMVIATVIGARLGHCLFYEPGYYLSNPVEILKIWQGGLASHGA
                     AVGIIFTLIIFARKNKVSFLWIIDRIAVVTALAGFFIRMGNLMNSEIFGKPTSLPWGF
                     IYERAGEIAQRTQPRHPSQIYEALAYLLIFALLLFVYRKKDGKPKEGILISLFLVLVF
                     GVRFFIEFLKEPQVGFEAQMSLNLGQWLSIPFVLAGIAGLILIRAKSIKTGRL"
     gene            complement(3809..4927)
                     /locus_tag="PKZ74_04795"
     CDS             complement(3809..4927)
                     /locus_tag="PKZ74_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM92109.1"
                     /translation="MAENFLRILVCPLDWGLGHATRCVPVITKMVEEGHQVTLAADGQ
                     ALGFLRSQFPDLPWKRFPGVTIRYPSAKSMVLKMLLQLPGIFLNIILENQRIKKIAGE
                     CRADVILSDNRFGLWHKKIHTIYMTHQVMIKAPDRLKWAEPWLYRIHLRFIRKYDECW
                     IPDFPGEPNLSGDLAHQYPVPPNGLFIGPLSRFTAIRASIKHREQTGGPALMVMISGP
                     EPQRSIFENIILRQLMENPGINAVILRGLPGEVRDLPAMEGVRIFNHLPDIDLLPMIL
                     NANTILCRSGYSTIMDLATLGRKAVLVATPGQTEQEYLANYLQETAQWKSVNQNDLIL
                     RRVMDPGNGPDESVETIRGEQLLYAAVRRLRTTGRRDK"
     gene            4978..6084
                     /gene="dprA"
                     /locus_tag="PKZ74_04800"
     CDS             4978..6084
                     /gene="dprA"
                     /locus_tag="PKZ74_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPM92110.1"
                     /translation="MQNKDSLIYAIALTLIPGIGDVQGKKLVAYCGGVEAVFREKKKD
                     LMRIPGIGNATADAIHRKGIFARAEEEIGFIRQYGINALFYLDPDYPERLKHCEDGPI
                     MVYFKGNAGLSRKRVLSIVGTRNPTDYGKSMCEQIIRDLSDLGVMTVSGLAYGVDTLA
                     HRVSLAAGIPTVGVLAHGLDLIYPFVNRPLAGKMIENGGLMTEFMSHTKLNRDYFPRR
                     NRIIAGMADATLVIESAAKGGALITAEIAGSYNRDVFALPGRATDLKSSGCNNLIRNN
                     KAALVQSAADICYLMGWDQDNHRAHSQPDLFTVMNEEEKNIYDLLMRLKEAGIDEIYL
                     QSGMTQSKVSSILLKLEFDGLVKAFPGKRFRLNN"
     gene            complement(6087..6770)
                     /locus_tag="PKZ74_04805"
     CDS             complement(6087..6770)
                     /locus_tag="PKZ74_04805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPM92111.1"
                     /translation="MEFLNYIYFVIPPAIAVLVFFYAKAQQNEWPAGLLIRAFVWGMF
                     SIVLVLTVQVIAAVFDLDNLTNLRRILFYSLVIMAFFSELGKFTILKVFFYPKKEFRT
                     PVDGILVSVMISLGFATMNNILSFVNIPHLSVSSVNALSAGPANVIFGLMMGFFIGLG
                     KLRKMRLVDSMTGLAAAIFFHALYDFCLLTRDYKLLWAFFIGSAIIAISLWIAALRIH
                     RDALEEGNL"
     gene            6837..7757
                     /gene="rsgA"
                     /locus_tag="PKZ74_04810"
     CDS             6837..7757
                     /gene="rsgA"
                     /locus_tag="PKZ74_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPM92112.1"
                     /translation="MEGIVTRSTGSWYMVTSDEGMVTPCKLKGNFRLKGIRTTSPLAV
                     GDRVEYEMTPGNDAGVIYRIFDRHNYIIRKATKLSRESHIIAANIDQLLLVATLAFPR
                     TSTGFIDRVLVTSEAYHIPAILVFNKIDMIVEELKEGLREMIEIYEHAGYRCLQVSAR
                     SGAGLDELMDAMKDRTSLLSGHSGVGKTSLINALQPGINLKIKEISSYHLKGQHATTF
                     PEMHRLSFGGYIIDTPGIKEFGLTDFGQFEVAERFPEMRALMHLCRYGNCTHSHEPGC
                     EVIRAVNDGRIHPSRYSNYLGILNGRDWTE"
     gene            7777..8439
                     /locus_tag="PKZ74_04815"
     CDS             7777..8439
                     /locus_tag="PKZ74_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPM92113.1"
                     /translation="MIAKEIITFDILPLNVNDSCSFALNAMDENRVNHLPLVRERELI
                     GLVSEFDLVNHPDQSEPLGNVRLSMPNAFISSYQHVFDVMKMMTELKLSLLPVVDQRN
                     LYQGAITLSALVRYLTLNMSVLNPGGMIILEVAESNYSMAEISQIVESNDVRIIGAFF
                     STKADSKLIDLTLKINATDLNPVIQTFERYHYTIKATFAEKDDLDELKERYDSLMNYL
                     NV"
     gene            8451..9905
                     /locus_tag="PKZ74_04820"
     CDS             8451..9905
                     /locus_tag="PKZ74_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM92114.1"
                     /translation="MSIDPAKYAIGPVWLFLALFLALAGIGHAQETDGSQDKGDRFIS
                     IDKIVLEGNRITRDPIILREIVFKEGDFIRSAALDSLIGCSRNNLLNTLLFNFVEINA
                     NHTDSSGSSVVVGVKVVERWYIWPTPIFKLSDRNFNVWWETRDPERLSYGFYIDWKNF
                     RGRKEELILRFQWGYDRLIQLQYQVPYLNRSKTMGLGVGFGFARQKESAFRTLNNKQE
                     FIRLDSGFARKDLFAYSQLTVRNNIHITHTIQAGFDQHHFSDSLIRSNPDFTNDTSTV
                     IRYFSISYQYKNDHRDFKSYPLKGYYADFGLVKNGLWTFPEKTINSIQITATYRKYWE
                     LNPRIYLATGINGYFSAGRQPYFVLGGIGYDRDIVRAYEYYLIDAEHFVILKNNVKFA
                     LIPNREHRFQFIRAEKFARIFYALYLNLFVDAGYGYYSQEFGQTTNDLQNSMLIGYGA
                     GLDFVTYYDVVVRLDFSVNYRNEAGFYLHFRAPI"
     gene            9965..10843
                     /locus_tag="PKZ74_04825"
     CDS             9965..10843
                     /locus_tag="PKZ74_04825"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPM92115.1"
                     /translation="MVIALFGKPISQENLKYVQSVIDKLLLKKCRVLFHRQFYESLAG
                     KVETGENIGFFDDMEDLAGKVDYFISIGGDGTILDAVTLVRDSGIPIIGINIGRLGFL
                     SSIGKDEILPAIDKVLDGDFRIDSRTLVALSQPKDLFGDLSYALNELTITRKDTTSLI
                     VLHVYIDDKYLNSYWADGLIIATPTGSTAYSLSSGGPILTPGSRNFVLTPIAPHNLTV
                     RPVVIPDMSSIRVIVEGREDQFLVSLDSRKATIHSNFELMVEKAAFSINMIQMREKDF
                     YMTIREKLKWGLDARN"
     gene            10897..11700
                     /locus_tag="PKZ74_04830"
     CDS             10897..11700
                     /locus_tag="PKZ74_04830"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPM92116.1"
                     /translation="MKKYLLIPVFTLIGVVTWAQTAEVGLIGGITYYLGDINPGIHFL
                     ASRPAYGGVARVNLNSRWTVKAEVVRGEIRGNDARGTTNDKRGLGFESKITDVGASAE
                     FNFFDYTTGSRKNILTPYIFGGVGMVFYNPEYNGIDLRSLGTEGQNAGFDGRSPYGKV
                     TFTIPFGLGIKYSINRRLGLTAEWGMRKTFSDYLDDVSTTYYLNGTQIDPGDPAEFLS
                     DPTRLHQPMQERGNPTTKDWYNFAGISLTYKFRLYNKNKCPNQWKSDYD"
     gene            11734..12489
                     /locus_tag="PKZ74_04835"
     CDS             11734..12489
                     /locus_tag="PKZ74_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPM92117.1"
                     /translation="MSYKEKLDLEKLPQHIAIIMDGNGRWAKLRGKPRTFGHKHGVTA
                     VREASEAAAELGVKYLTLYAFSTENWKRPKFEVDALMHLLLQSINKETKTLNKNRIRL
                     NAIGNLEALPESIYKQLIKTIEQTAANDRMTLTLALSYSSRWEIVNATKRIVSEFAEG
                     KITLENITADYFTSMLPSFPLPEPELLVRTSGENRISNFLLWQIAYSELIFTPKLWPD
                     FRKEDLYEAIFNYQHRERRFGKTSDQINNHQIP"
BASE COUNT         3416 a         2909 c         2996 g         3236 t
ORIGIN      
        1 aaattgcagg tgggtcatcc gccaggggct ccgacgttcc agcttatcgg aagatttttc
       61 tccctgtttg catttggaaa taccgccaat gtggccatga tgatcaatac tatgtcggcc
      121 atggtcagcg cattgacgat catgttcctt ttctggacca tcacgctgct ggcccggaaa
      181 atggtgaaag ccgatgccgg atataccctg acacaggtca tactgctttt cggcagtgcg
      241 tttgtcggcg ctatggcttt tactttttcc gattctttct ggttctccgc ttcggaaggt
      301 gaagtctatg ccatgtcttc cttgtttacc gccatcactt tctgggcgat cctgaaatgg
      361 gaagtggtcg ccgatgaaaa gcattccctc cgctggctca tcctgatagc tttcctgatc
      421 gggctttcca ttggaatcca cctcctgaat ttgctggcta tccctgccat cacattcgtt
      481 tattattacc gtaaatacaa acccagccgt aaaggcttgc tgttagccgg agtggtatcc
      541 gttctgatcc ttgccctgct tatgtatttc ttcatccccg gaattgtgaa actggccggc
      601 tctttcgagc tgttttttgt caactccatc ggccttccgt tcaactcggg aaccattatc
      661 tactttgccc tcattatcgg tttgattgtc tgggggctga agtatacttc gaaaaaaggc
      721 aaaagtgttt taaattctat catactcgct tttgttttcc ttctgatcgg ttattcatca
      781 tttatcattc tgattatccg ctcaaatgcc aacaccccta ttgatgaaaa taatcctgaa
      841 gatgccattt cgttgctgag ctacctcaac cgcgaacaat acggaaccta cccgctttat
      901 tacgggcaat attacaatgc tccagtggtt gattacaagg atggtacgcc cgtttattta
      961 aaagacagaa gcaaaggaaa atatgtgatt acggatgacc ggaaggaatc tttgccggtt
     1021 tatgattccc ggttcaccac cctttttccc aggatgtgga gcgaccaaaa gagctcccat
     1081 atcaacaaat acaaggaata cggaaaaata aaagggaccc cgatcaccgt cagtaaaccg
     1141 gacggtacga ctgaaacctt gtataaaccg acattcgtgg aaaatatgcg gtttttcttc
     1201 agctatcaac tgggacatat gtatttccgc tatttcttgt ggaattttgc cggtaggcag
     1261 aatgatatcg aaagccaggg agaaccgcaa aacgggaact ggatttcggg attcaatttc
     1321 ctggataatg gccggctcgg gaatcagtcg gatctgccgg aaagtatgca gaatacggct
     1381 agaaacaagt tttacctcct gccgctttta ctgggtatag ctggtatact ttatcacttt
     1441 aagaagaaca ggaatgatac ctgggtggtg tttttattgt tcctgatgac cggtattgcc
     1501 atcgtggtat tccttaacca gaccccgtac caaccccgcg aaagggatta tgcatatgtt
     1561 ggttcgtttt atgcattcgc gatctggata ggcctggggg tgatgggact ggcggaattt
     1621 atacagaaat acgtcagaaa acaagaagta gccgtagccg cagccgctgt cggcctttct
     1681 ttcctgctcg taccgggcat aatggcacac cagggctggg atgaccacga ccgttccgga
     1741 aaatacgccg ccagggattt tgccatgaat tacctgcggg gttgcgataa aaattccgtg
     1801 ttggtgactt tcggcgacaa cgatacattt cctttatggt acgtccagga ggttgaaggg
     1861 ttccgtactg atgtcagggt agtgaatcat atgcttgctt cgggacactg gtatgttcag
     1921 cagttgttct caaaggtcta tgattcggat ccactgcctt ttaccctgag aatagaccag
     1981 tataacaacg gggtaaataa ttatgtgccg gtatatgaga ttcccagcct ggccaacgaa
     2041 tataccgagt tgtcggaatt gatccggttt gtggcattgg atagcgacaa gagtaaaata
     2101 ccgatctcat caactaaaaa gatcaatttc ttcccgtcaa ggaaggtgaa aatgacggtt
     2161 gacgctaaaa aatgcgtgga gaacgggatt gtgccacctg aactggccga ccggatcgtt
     2221 ccggtcattg aatgggagat aaagcaaaat gcactttata aaaacgacct tgcggtcctc
     2281 gatttcctgg catcaagcaa ctgggaaagg gcgttctata cagcgaatcc gtcgagcctg
     2341 ctggattttc ttgacattga ccaatacctt caccaggagg ggctggtcta taaattcatg
     2401 ccggtaaaag cggataacta ttaccgcggt gtgggaggag ttaacggaat gaagacttat
     2461 gagaacttca tgaattgccg ttggggtaac ctcaaccaac ccaatatcct ggttgaccgg
     2521 gaaagttacc ggaacagccg gcttccgagg cagaactacc tgcgggctgc agaaacactc
     2581 ctcatcaatg gtgacaaaca aaaagctgtt gagttgcttg accgatgcaa tgaatttttc
     2641 cctgacaata aaatcaatta cgatatgatc atgatcccgt ttgccgaagt ctattatggg
     2701 gcgggtgaac cggaaaaagg caacgagatt atcctaaagc tgcttgatta ttatgcggat
     2761 gacctccggt attacaatac ggtgaaacca tcattcgtgg aaaaatatta ctctgactcc
     2821 ataaccagga tcatgaggat attgcgcgaa atcgcacagg tgaccaggac caacggtcag
     2881 cctgaaatat cagcaaaggc aaccgaattg atggaatcct acaaatagtt aaaggcggcc
     2941 cgtctttata ctttttgccc ggataagaat cagaccggct attcccgcaa gtacaaaagg
     3001 tatactgagc cattggccca ggttcaggct catttgagcc tcaaatccta cctgtggttc
     3061 cttcaggaat tcaatgaaaa acctgactcc gaatacaaga acaaggaaaa ggctgatcag
     3121 tatcccctct ttcggtttac catccttttt tctgtatacg aaaaggagga gggcgaaaat
     3181 cagaaggtat gccagcgctt cataaatctg gctggggtgc cggggctggg tccgttgggc
     3241 aatttctccg gcgcgctcat aaataaaacc ccagggaaga gaggtcggct taccgaaaat
     3301 ctccgaattc atcaggtttc ccatccggat gaaaaaaccg gccagggctg taacaacggc
     3361 aatccggtcg ataatccata aaaaggaaac tttgtttttt cgggcaaaga tgatgagggt
     3421 gaatataata cccactgcag ccccgtgact ggccagtcct ccctgccaga ttttcagtat
     3481 ctcgaccggg ttggaaaggt agtaaccggg ttcataaaac aggcaatggc ccagcctggc
     3541 cccgatcact gttgcaataa ccatataggt tgtcaactga tccagcaatt ttacggggat
     3601 cttttccctg cgaaagaaac cgaggatgat aaaatatccg cagacgaatc ccagggcgaa
     3661 cagcacgcca taccagcgaa ccgcaaaggt gccgatccgg aatatctcgg ggtcgggcga
     3721 ccaggttatc gataatagat ccatagaata ctcgggcaat ttatttatcg ccaaaaatag
     3781 aaataatgaa cgatgctgat aaaattattt atttgtcccg tctgcctgtt gttctaagac
     3841 gtctcacggc ggcataaagc agttgctccc cccggattgt ttcaacgctt tcatccggcc
     3901 cgttgcctgg atccatcacg cgtcgaagta tcagatcgtt ttgatttaca gatttccact
     3961 gcgctgtttc ctgtaaataa tttgcaaggt actcctgctc tgtctgcccg ggagttgcga
     4021 caagtacagc ttttctgccc agcgttgcga gatccattat agtggaatag cctgaccggc
     4081 acaaaatcgt atttgcgttc aggatcatcg gcaggagatc aatgtcgggt aaatgattga
     4141 atatcctgac gccttccatt gcaggaagat ccctgacctc acccggaagc cccctcagga
     4201 taacggcgtt gatccccgga ttttccatga gttgccttag aattatattt tcgaaaatcg
     4261 agcgctgtgg ctcagggccc gagatcatta ccatcaaagc gggaccaccg gtctgctccc
     4321 ggtgttttat cgaggcccga attgccgtaa agcgggaaag agggccgatg aaaagcccgt
     4381 ttggcgggac cgggtactga tgggcgagat cgcctgaaag attcggttct cccggaaagt
     4441 cagggatcca gcattcatcg tatttccgta taaacctgag atgaatgcgg tacagccagg
     4501 gttctgccca tttcagcctg tcgggggcct tgatcatgac ctggtgggtc atgtaaatag
     4561 tgtgaatctt tttatgccat aatccaaacc ggttgtcaga caggatcacg tctgcccggc
     4621 attctccggc aatcttttta atgcgttgat tttccaggat gatattcaga aagataccgg
     4681 gaagctgaag caacattttc aaaaccatgc tctttgctga aggataacgg attgtaactc
     4741 ctggaaaccg tttccatggc agatcgggaa attgactcct aagaaatccg agggcctggc
     4801 catccgcagc gagtgttacc tggtgacctt cttccaccat ttttgtgatc acaggcacac
     4861 aacgcgtcgc atgacccagt ccccaatcga gcggacagac aaggattcgg agaaaatttt
     4921 cagccaaccg ggtatttttt gcccgacaaa ggtattaata agaaaaacct gttgattatg
     4981 cagaacaaag attcattgat ttatgccata gccctgacgc tgataccggg tatcggggat
     5041 gtccagggta agaaactggt tgcctattgt ggtggggtgg aagcggtttt ccgggagaag
     5101 aagaaagatc tgatgcgaat tccggggatc ggcaacgcta ccgccgatgc gatccatcgc
     5161 aaggggatct ttgccagggc ggaagaggag attggcttca tccgtcaata cggtattaat
     5221 gcattatttt accttgatcc cgattatccc gaaaggctga aacattgcga agatggcccg
     5281 ataatggtct attttaaagg aaatgccgga ctctcccgga aacgcgtact tagtatcgtc
     5341 ggtacacgta acccgaccga ttacggcaaa tccatgtgcg aacagatcat ccgcgatttg
     5401 tcggacctgg gcgttatgac ggtcagcggg ctggcatatg gtgtcgatac tttggcccac
     5461 cgcgtctctc tggccgccgg tattcctacg gtaggcgtgc tcgctcatgg cctcgacctg
     5521 atctacccct ttgtcaatcg tcccctggcc gggaaaatga tcgaaaacgg tggattgatg
     5581 accgaattta tgagccatac caaactgaac cgtgattact ttccgcggcg caaccgcatt
     5641 attgcgggta tggccgatgc gaccctggtt atagaatccg ctgccaaagg aggagcactg
     5701 atcacggcgg aaattgccgg ctcttacaac agggatgttt ttgcccttcc gggccgggca
     5761 accgatttga aatcaagcgg atgcaataac ctgatcagaa acaacaaagc ggcactcgtg
     5821 caatccgctg ccgatatctg ttatctcatg gggtgggacc aggataacca tcgggcgcac
     5881 agtcagcccg atctttttac tgtaatgaat gaggaagaaa agaatatcta tgatcttctc
     5941 atgcggctga aagaagccgg aatcgatgaa atatacctgc aatcgggcat gacgcagagc
     6001 aaagtctcgt caatattgct gaaactggag tttgacggat tggtgaaagc ttttcccggt
     6061 aagagattca gactgaataa ctaaaatcag agattccctt cctcaagggc atctctgtga
     6121 atccgtaatg ccgcaatcca taatgagatg gctatgatgg ccgacccgat gaaaaaagcc
     6181 caaagcagtt tataatccct ggtcaggagg caaaaatcgt acaaagcatg aaaaaagatc
     6241 gctgctgcaa ggcctgtcat ggagtcgacc agccgcatct tcctcaattt tcccaaaccg
     6301 atgaagaatc ccatcatcaa accgaaaata acattggccg ggccggcgct gagtgcgttg
     6361 acgctgctga cagacagatg gggtatgttg acgaaggaga gtatgttgtt catcgtggcg
     6421 aaacccaacg atatcatcac tgaaacgagg ataccatcga cgggagtcct gaattctttt
     6481 ttcggataga aaaaaacctt gagaatggta aactttccca attcagagaa gaaagccatg
     6541 atcacgagtg aataaaacaa gattcgcctg aggttggtca gattatcaag gtcaaaaacc
     6601 gcagctataa cctggacagt cagcacaagc acaatactga acattcccca gacaaaggca
     6661 cggatcagca atccggccgg ccattcattc tgttgggctt tagcataaaa aaataccaga
     6721 acagcaattg ccggtggaat gacaaaataa atataattca gaaactccat aagattgaag
     6781 gttatcgggg attcaaaatt agcaattgtt tgtaatttta caaattaaaa tatttcatgg
     6841 aagggatcgt cacacgttca acgggcagtt ggtatatggt aacttctgac gaagggatgg
     6901 tcaccccctg caagttgaaa ggcaatttcc ggctgaaagg cattcgaaca accagcccgc
     6961 tggccgttgg cgaccgtgtt gaatacgaaa tgactccggg aaacgacgca ggtgtgattt
     7021 accgtatttt tgaccgtcat aattatatta tccgcaaagc aaccaaactt tcccgggaat
     7081 cacatatcat cgcagccaac atcgaccagt tgctcctggt ggccactctt gcgtttccca
     7141 ggacatccac cggctttatc gaccgtgtac tggtcacttc agaggcttat catattccgg
     7201 ccatcctcgt cttcaataaa atagatatga tcgttgagga actgaaagaa gggctccggg
     7261 agatgataga aatatatgag cacgccggtt acaggtgcct gcaggtatcg gccaggagcg
     7321 gggcagggct ggatgaactg atggatgcca tgaaagaccg gaccagcctg ctttcaggtc
     7381 attccggtgt gggtaaaacg agcctcatca atgccctgca acccggcatt aacctgaaaa
     7441 taaaggagat atcatcctat cacctcaaag gccagcatgc cactacattt cctgagatgc
     7501 accgcctctc tttcggcggg tatataatcg atactcccgg aatcaaggaa ttcggcctga
     7561 ccgatttcgg gcagttcgaa gtcgccgaac gatttcccga aatgagagcc ctcatgcatc
     7621 tttgccgtta tggcaattgc actcactcac acgaaccggg ttgtgaagtg atccgtgcag
     7681 tcaacgacgg aaggatccat ccttcgaggt attccaacta ccttgggatt ttgaatggcc
     7741 gtgactggac cgaataatca ttttaaacaa attgttatga tcgccaaaga aataatcacc
     7801 ttcgatattc tgcccctgaa tgtgaatgat tcttgctctt tcgcccttaa tgccatggat
     7861 gagaaccggg tcaaccatct gccgctggtt cgtgaaaggg aattgatcgg acttgtgtcg
     7921 gaattcgacc tggttaacca ccccgatcaa tctgagccgc tcggaaacgt caggctctcc
     7981 atgccgaacg cgttcatctc ttcgtaccag catgtgttcg atgtgatgaa aatgatgacc
     8041 gaactgaagc tttcgctgct cccggtcgtt gaccaacgga atctttacca gggagccatc
     8101 accctttccg ctttggtcag atatctcaca ttgaatatgt cggtgcttaa cccgggcggg
     8161 atgattatac tggaggtggc tgaaagcaat tattcaatgg ccgagatatc ccagatcgta
     8221 gaatcgaacg atgtgcggat catcggtgct tttttcagca ccaaagcaga ttctaagctt
     8281 atcgacctta ccctcaagat caatgcaaca gaccttaatc ccgtcatcca gacattcgaa
     8341 agatatcact atacaattaa agccactttt gccgaaaaag acgacctgga tgaactgaaa
     8401 gagcgttatg attcgttgat gaattacctc aacgtatgaa atgatttcat ttgagcattg
     8461 atccggcaaa atatgctatc gggcctgtct ggcttttcct ggcgctgttc ctggcactcg
     8521 caggcattgg ccacgcccag gaaacagacg ggagccagga taaaggcgac cgctttattt
     8581 ccatcgacaa aattgttctc gaaggtaacc ggatcacaag ggatcccatt atcctgaggg
     8641 aaattgtatt caaggaaggt gattttatcc gttctgccgc cctcgactca ttgatcgggt
     8701 gttcaaggaa taacctgctg aatacgcttc ttttcaattt tgtcgaaata aacgccaatc
     8761 acactgattc gtcagggagt agtgttgttg tcggggttaa agtggttgag agatggtaca
     8821 tctggcctac acccatattc aaattatcag acagaaattt taacgtatgg tgggaaaccc
     8881 gtgatcctga aaggcttagt tatggatttt atatcgactg gaaaaatttc aggggaagaa
     8941 aagaggaatt gatcttaagg ttccagtggg gatatgaccg gttgatacag ctccagtacc
     9001 aggtgcctta tcttaaccgg tcgaaaacga tggggttggg cgtcggtttt ggttttgccc
     9061 gccagaagga atcggcattc cggacgctga acaataaaca ggagtttatc cgccttgact
     9121 ccgggtttgc gcgaaaggat ctttttgcct acagccagtt gacggtccgc aacaatattc
     9181 atattaccca cactatccag gccggattcg accagcatca tttttccgac agcctgatcc
     9241 gctccaatcc cgattttacc aacgacactt ctaccgttat acgctatttt tcaatttctt
     9301 accagtataa aaacgatcac cgtgatttta aatcatatcc cctgaaaggt tattacgctg
     9361 attttggtct cgtcaagaat ggattgtgga cctttcctga aaagaccatc aattccatcc
     9421 aaataacggc cacttaccgg aaatactggg agctaaatcc ccggatttac cttgcaacag
     9481 gtatcaatgg gtatttttct gccggccgcc agccttattt tgtcctggga ggaattggct
     9541 acgaccgtga tattgtaaga gcatatgagt actatctgat tgatgccgaa cattttgtta
     9601 ttttgaaaaa taatgtcaag ttcgccctga tcccgaaccg ggagcatcgt tttcaattca
     9661 tcagggccga aaagtttgcg cgaatttttt acgccctcta ccttaattta ttcgtggatg
     9721 ccggatacgg ttattattcg caggagttcg ggcagacaac aaatgacctg caaaattcta
     9781 tgctgatcgg atatggtgca gggctcgatt tcgtcaccta ttatgatgtg gttgtcaggc
     9841 tcgatttttc cgtcaattac aggaatgaag ccggatttta tcttcatttc cgggctccga
     9901 tataatctga tgaaaatgat cattattcgt tacttttgca caagccgttt aaaatgataa
     9961 gcatatggtg attgcgctgt tcggtaaacc gatttcccag gaaaatttga aatatgtgca
    10021 aagtgtcatt gataagctat tgctgaagaa atgcagagtg cttttccaca ggcaatttta
    10081 tgagtcgctg gcggggaagg tggaaacagg tgaaaatatt gggttcttcg atgatatgga
    10141 ggatctggcc gggaaggtgg attattttat ttccattggc ggtgacggga ccatcctcga
    10201 cgccgttaca ctcgtcaggg attccggaat cccgatcata ggcattaata tcggccggct
    10261 gggttttttg tcgagtattg gtaaggatga gattttgcct gccattgata aagtcctgga
    10321 tggggatttc aggatagatt cccggacact tgtcgcgctt tcgcagccga aggatctttt
    10381 tggcgatctc agttatgctt tgaatgaact gactatcacc cgtaaagata cgacttcact
    10441 gatcgtgctc catgtttata tcgatgacaa gtatttgaac tcttattggg ccgacggtct
    10501 gatcatcgcc actccaaccg gttcaacggc ctattcgctg agcagcggcg gcccgattct
    10561 aactcccggt tccaggaatt ttgtcctgac tccgattgct ccgcacaatt taacggtcag
    10621 gcctgtcgtt atacctgaca tgtcgtcaat ccgggtcatc gttgaaggac gggaggacca
    10681 gttccttgtc agccttgatt ccagaaaggc aactatccat tcaaattttg aacttatggt
    10741 tgaaaaggcc gcattcagca ttaatatgat ccagatgcgt gagaaggact tttatatgac
    10801 catcagggaa aagctcaaat ggggccttga cgctcgcaac tgaacagttg attaatcttt
    10861 aatttttttt gatatttttg cggcctgcaa gataagatga agaaatacct gttaattcct
    10921 gtatttacgt taatcggagt ggtaacctgg gcccagacag cggaagtcgg cttgatcggc
    10981 ggaatcacct attacctggg agatatcaac cccgggatcc attttctggc gtcacgacca
    11041 gcctatggcg gggtggcgcg agtaaatctt aattcgaggt ggaccgtgaa agccgaagtg
    11101 gtccgcgggg agatccgggg caatgatgcc cggggcacaa ccaatgataa aagaggcttg
    11161 ggattcgaat ccaaaattac cgatgtggga gcctcggccg agtttaattt cttcgattat
    11221 acaaccggga gcaggaagaa tatcctgaca ccgtatatct tcggaggcgt cggaatggtt
    11281 ttttataatc cggaatataa tggtatagat ttgcgcagcc ttggaacaga agggcagaac
    11341 gccggcttcg acggacgttc gccctatggc aaagtgactt ttaccattcc ctttgggctg
    11401 ggaataaaat atagcatcaa ccgtcgactg ggtttgactg ctgaatgggg catgaggaaa
    11461 actttttccg attacctgga cgatgtcagc acaacatatt atctaaacgg aacacagatc
    11521 gatccgggtg atccggcgga atttctctcc gatccgaccc ggctccatca accaatgcaa
    11581 gaaaggggta atccaacaac caaagattgg tataactttg caggaatttc actgacttat
    11641 aaattcaggc tttataataa aaataaatgc ccgaatcaat ggaaatcaga ttatgattga
    11701 tgggttgtta aagaggcaag acgttaaaaa ataatgagtt ataaagaaaa actggatctg
    11761 gaaaagctac cgcaacacat tgctatcatc atggatggaa atggacgttg ggctaaattg
    11821 cgtggcaaac ccaggacctt tggccataaa cacggcgtga ccgctgtcag ggaagcctct
    11881 gaagcagcgg ctgaattagg cgttaaatat ctgacgctct atgctttctc cactgagaac
    11941 tggaagcggc caaaatttga agtggatgcc ctgatgcacc ttcttttaca atcgatcaac
    12001 aaagaaacca aaacactgaa taaaaaccgg atcagattaa atgccatagg taacctggaa
    12061 gccctccctg aaagtatcta caaacaactc ataaaaacaa ttgaacagac tgcagctaac
    12121 gacaggatga cacttaccct ggccctgagt tacagctcac gctgggagat cgtgaatgct
    12181 accaaacgaa tagtgtcgga gtttgccgag ggcaagataa cgctcgaaaa tatcaccgcc
    12241 gattatttca catccatgct tccttcattc ccacttcctg aaccggaact ccttgtccgc
    12301 accagcgggg aaaacaggat cagcaacttc ctgctatggc aaattgctta ttcggaattg
    12361 atatttaccc cgaaactatg gcccgacttc agaaaagaag acctgtacga agccatattt
    12421 aattaccagc accgcgaacg aaggttcggt aaaaccagtg accagatcaa taatcatcaa
    12481 ataccctaaa gtgaactacc ggaaaattct gcagcgaaac cctgttctta ttccccgtgt
    12541 taaatcgggg aaattat
//
LOCUS       DAOPNH010000062        12530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5237_length_12530_cov_5.518637, whole genome shotgun sequence.
ACCESSION   DAOPNH010000062 DAOPNH010000000
VERSION     DAOPNH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5237_length_12530_cov_5.518637"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="bamD"
                     /locus_tag="PKZ74_04840"
     CDS             complement(<1..403)
                     /gene="bamD"
                     /locus_tag="PKZ74_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPM92118.1"
                     /translation="MFFNRLNILLVIAVLVLAGCGKHQKLLKSTDNEAKYAAAIGYYE
                     NNDYYRALQIFQQLINFYQGTDKAEKMQFYYAYCYYHQRDYVLASYYFKRFVTNYPRS
                     PMAEEAMYMNAYCYFLDSPPSNLDQTNTFTAL"
     gene            551..1801
                     /locus_tag="PKZ74_04845"
     CDS             551..1801
                     /locus_tag="PKZ74_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM92119.1"
                     /translation="MDIFEKIANKMGPLGKYYESAHGYYMFPKLEGDISDKMTFNGKE
                     RLVWSLNNYLGLANHPEIRKADADAAARFGLAYQMGARMMSGQTKYHEQFEKEVAEFV
                     GKEAAYLLNFGYQGMVSIIQSLVDRKDVIVYDSEAHACIIDGMLLHIGKRFVYPHNNM
                     QSLEKQLQRATKITDETGGGILVITEGVYGMAGDLGNLREIVALKKNYNFRLLVDDAH
                     GFGTMGKTGQGTGEFLGVQDEIDVYFATFAKAFAGIGAFIASDKGIIKYLMYNLRSQI
                     YAKSLPVAMTIGALKRLEIMRKEPQHRERLWTIVNALQKGLREKGFNIGKTESPVTPV
                     YFTGGVSEATQITYDLREKYNIFCSIVVYPVVPKDVIMLRLIPTAVHTLEDVEYTISA
                     FEKVKQKLDAGEYSDGKIKAMQVF"
     gene            1835..3109
                     /locus_tag="PKZ74_04850"
     CDS             1835..3109
                     /locus_tag="PKZ74_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92120.1"
                     /translation="MSPGISHKKRNYSSVYKIYRRLRFMRHRKKQLKQQIKQQLIRET
                     DEKKLAEARLREFRIQEKKQFKIKAKADKRADKERKAALKEEMKQMAIMARLEKEKEV
                     KEAIERRKKQWQLEKLETQRLKEEKRLQRIQVRTKFSWKVYFKNAVIKFKSGKAKRRH
                     NALITINSTVFFIMSYLALFFISQAVTVIAAGFFNYPATVYYFEIYFNISPEAWYHDS
                     VKTIFSAGPLVNFILGVICLIIYHKLKESPVTFKIFFLWGFLHGVNMLFGAMLVGTLF
                     ETGVGHVISWMYIMDTGRLLYSILSIFLLVIAGMIATKPFLFSANSYFNEINTGNRLA
                     FILSQVFWPFLTGNIIMILVRQPRLVFYDTFILFTLVISVLAVLFTFSGHNELYFEEE
                     EKKPGIAWIATGILILALIFFRVVLGIGIRLG"
     gene            complement(3119..4840)
                     /locus_tag="PKZ74_04855"
     CDS             complement(3119..4840)
                     /locus_tag="PKZ74_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM92121.1"
                     /translation="MNKFRFYNTVLILLLFAFNISTFSQNSDSKSFLDSLYNNEIEQY
                     FGFDLRQAQGLEYLGKFISIDKVDNNGRVFAYANREGFSKFLETGLSFDLLPHPGDFS
                     GYLNMKEKVDIREISEWDFYPTYDAYIDMMNQFADQYPSICQLVTIGTTIQGRQILAV
                     KISDNVGLSENEPQFLYTSSIHGDETTGYILMLRLADYLLSNYNIDPRLTNLVNEIEI
                     WINPLANPDGTYFGGNNTVNGARRYNYNGIDLNRNFPDPEDGPHPDGYPWQTETVHFM
                     NLAQNNHFVSSANIHGGEEVCNYPWDTWSRLAADDSWWQYVCHEYADTAQLHSPAGYM
                     SDFDDGITNGYQWYTISGGRQDYMNYFHQAREFTLEISDQKLLSPGLLPAWWEYNYRS
                     LLNYLEQSMFGLRGTVRDSVTGWPIRAEVYAVLHEIDSSWVYSSLPAGNYHRLLHEGT
                     YTIRYSAPGYETKVIQNVEIVNRQATILDVKLVPEGVGGINNPRVSNRIDIYPVPVTN
                     GYLNFKTDLNIRSVFIYDTSGKLLIQITEPINEGFLDIHSLKNGIYFAGFFTNQGFGF
                     KKIAVMR"
     gene            complement(4864..5763)
                     /gene="dapA"
                     /locus_tag="PKZ74_04860"
     CDS             complement(4864..5763)
                     /gene="dapA"
                     /locus_tag="PKZ74_04860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPM92122.1"
                     /translation="MYDYLKGTGVAVITPFHDSGNIDFGSFEKIIEHLITGGINYIVA
                     LGTTGESAALSKDEKVAVLDFVIDTVNKRVPVVAGIGGNNTQEVINTIKATNFEGIEA
                     ILSVCPYYNKPQQKGLYNHYKAISGASQVPVILYNVPGRTSVNITAETTLKLAADFTN
                     IIGIKEASGNLMQCMEIINNKPSGFYVISGDDGLTLPLISLGADGVISVVANAFPKQM
                     TEMVNLCLRGKFTKARALHYNLLGFTNALFMDGNPSGIKAAMELLGLCKSNVRLPLAK
                     VNKNVHSMIAEHTDFLVKNQVEL"
     gene            complement(5756..6337)
                     /locus_tag="PKZ74_04865"
     CDS             complement(5756..6337)
                     /locus_tag="PKZ74_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92123.1"
                     /translation="MALFNNIRLRIAGYILQKGITSLKRNKKLVSLNTAGSVGLLFEL
                     TDHSVYYAIQDFFQKLQENKIKVKALGYSGTKNATSQFLPVLSFDFISGKDLNWLLIP
                     KAKCVTDFIETDFDICINIASEDIFPLKYIAGMSKAKLKAGAYNNVAADDNFKALSQI
                     YDIMLKSEPIHNQISFLGIINEYLSILNSKGNV"
     gene            complement(6398..8038)
                     /locus_tag="PKZ74_04870"
     CDS             complement(6398..8038)
                     /locus_tag="PKZ74_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM92124.1"
                     /translation="MRRLAFYGLLLAMISSCAEQKPDESGLTVFRYNEFSNISSLDPA
                     FAKDQASIWACNQIYNGLLQLDEDLNILPCIAHSWNIDTTGRIYTFFLRNDVFFHDHP
                     AFPGGIGRKVTAHDFVYSLKRLQDPKLASPGIWVLNKLEKEDGEPAIAALNDTILQIR
                     LKEPFSPFPGILTMIYCSVVPKEVVAEKGIEFRIHPTGTGPFRFKMWKENVKLVLVKN
                     TGYFEKDSNQQRLPYLDAIAISFLIDKQAAFLEFIKGNLDFISGLDQSYKDELLTKDG
                     HLKAGYQDKFMMMTEPFLNTEYVGMLVDSLTDAGRNSPLMNKKVRQAINYGIDRRKMI
                     RYIRNNIGVPGLHGIIPPGLPAFDSSEVFFDYDPQKAQALIREAGYGPANPLPAITLH
                     TTAEYTDIFKYMQHQLSSLGISVKIEVNPAATLMQLKAYSRINFFRASWIADYPDEEN
                     YLSLFVSTNAAPAGPNYTRYNNPVFDSLYKASQLVGDPVIRKDIYRQMNGLVMEDAPV
                     IILYYDQVVRFSWTYIQNLGTNPLNMLNLKSVKKIQEE"
     gene            complement(8098..10572)
                     /gene="lon"
                     /locus_tag="PKZ74_04875"
     CDS             complement(8098..10572)
                     /gene="lon"
                     /locus_tag="PKZ74_04875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPM92125.1"
                     /translation="MDDLFNINGLSDAVDNDTEFIPLLSSEEEELMHSEKLPESLPIL
                     PLRNTVLFPGVVIPITVGRDKSIRLIRESYRNDRTIGVVAQKDSAIEDPGEKDLYNVG
                     TVAHLIKILQMPDGNTTVIIQGKKRFEIEGFIQNEPYFRAKIKDLTDFDKNLEQDEHF
                     LALIGSLKDLAIQIIKLSPNIPSEAAFAIKNIDSPFFLINFVSSNLNIDLVDKQKLLE
                     TPDLTKRANEVLTAMARELHMLELKNQIQDKVKLDLDKQQRDYLLNQQLKTIQEELGG
                     SPHEQEINDLKAKAAKKKWTKKIAEIFDKELKKLQRMNPQAAEYSIQMNYLETVVELP
                     WNEFTNDDFNLKKAQNILDEDHYGLEKIKERIIEHLAVLKLKNDMKSPILCFVGPPGV
                     GKTSLGKSIARALGRNYIRVSLGGLRDEAELRGHRKTYIGAMPGRIIQSLRKAGSANP
                     VFVLDEVDKVSGNNFQGDPQAALLEILDPEQNFEFYDNYLEINFDLSRVMFIATANNM
                     STIHPALRDRMEVIDLSGYLLEEKVEIARKHLIPKQIREHGLKEKHVAFPKKTIEKII
                     DDYTRESGVRSLEKVIAKIVRNRARLVAMEEGYTPKLSNQDIETIMGAPRYQKDRDSR
                     IDVPGVVTGLAWTAAGGEILFIEVSLSRGKGNLTLTGNLGDVMKESAAIAFEYLKAHT
                     DSLGIDPDVFTNWNVHIHVPEGATPKDGPSAGITMLTALASAFTQRKIRPELAMTGEI
                     TLRGKVLPVGGIKEKILAAKRAKIKNIILSQENRKDIEEIKKEYVEGLNFIYIDKMID
                     VIAHALLPDKIKNPLDFRIKPETFSS"
     gene            complement(10722..11903)
                     /locus_tag="PKZ74_04880"
     CDS             complement(10722..11903)
                     /locus_tag="PKZ74_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HPM92126.1"
                     /translation="MNFILFDNFRRSNLFPLTYLRPVADIRTGILTIREKWERYLGQK
                     TSTLTESYLRKKFPVVREEDNIMINGSVCPNKQLLEEILCLKTGEVLFHGDAIIAMRL
                     HAVELEKLDDEHIDDVNMVESKSDILKITHTWDIFSQNDRAIREDFNLLTHGRVSGPV
                     SKSNQVIGNEVFIEKNAKVECCILNSSTGPIYIGEDAEIMEGSLIRGPFGLGAHATVK
                     MGAKIYGPTTIGPHCKVGGEINNSVFFGFSNKAHDGFMGQSVIGEWCNIGADTNTSNL
                     KNTYETIKLWNYADQTFVETGLQFCGLIMGDHSKCGINTMFNTGTVVGINSNIFGSGY
                     QRNFIPSFSWGGPSGIKSYHIDRAVSVARKVYQRRGLEFDQTEEDILRDVYNLTKEPL
                     P"
     gene            complement(11985..12260)
                     /locus_tag="PKZ74_04885"
     CDS             complement(11985..12260)
                     /locus_tag="PKZ74_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPM92127.1"
                     /translation="MKKNIHPKDYRFVIFKDMSNNYSFLTKSTAKSKETATWEDGKEY
                     PLIKLEISNTSHPYFTGKMKLVDTAGRVDKFKNRYKQHLEKRESASE"
     gene            complement(12344..>12530)
                     /locus_tag="PKZ74_04890"
     CDS             complement(12344..>12530)
                     /locus_tag="PKZ74_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92128.1"
                     /translation="TEPYRSVDSLDLILREQQDSLQCVVAGGKAFKGAVPFGKSQMPE
                     LWDYADGIDTMEFLFSL"
BASE COUNT         3349 a         2744 c         2761 g         3676 t
ORIGIN      
        1 taagggccgt gaaagtattg gtctgatcca ggttagaagg cggtgaatcc agaaaatagc
       61 aataggcatt catgtacata gcctcttctg ccatggggct cctgggataa ttcgtaacaa
      121 aacgtttaaa ataataactg gccagtacgt aatcgcgctg atggtaatag cagtatgcat
      181 aataaaactg cattttttcg gccttatcgg tgccctggta gaagttgatc aattgctgga
      241 atatttgcag ggcgcggtaa taatcattat tttcataata accgatggcg gctgcatatt
      301 tagcctcatt gtcggtactt ttcagtagtt tctggtgctt accgcatcca gccagcacaa
      361 gtacagcaat aaccaggaga atattcaggc ggttgaaaaa catcgtgcaa aagtacaaaa
      421 aattgattat ctgaataacg ctccggttaa tcaattatta tcctgtgcca ttcaatgaat
      481 tctacatgaa taattaataa tttagcggcc ggaatttatc attcaatcat taacttttaa
      541 tatcaaagac gtggatattt tcgaaaaaat tgccaataag atggggccat tgggtaagta
      601 ttacgaatca gctcatggtt attacatgtt tccgaaactg gagggtgata tctccgataa
      661 aatgactttc aacggaaaag aaaggctggt ctggagcctg aataattatc tcggactggc
      721 caaccatccg gagatccgca aggcagatgc cgatgcagcg gcccggttcg gactggccta
      781 ccagatgggc gcgagaatga tgtcggggca aacaaaatac catgaacaat tcgagaaaga
      841 agtagcagag tttgtgggta aggaagccgc ctaccttcta aatttcggat accaggggat
      901 ggtatccatt attcaatccc ttgttgaccg taaagatgtc attgtttatg attcggaagc
      961 gcacgcctgt atcatcgatg gtatgctttt acatatcgga aaacggtttg tgtatcctca
     1021 taataatatg cagagcctcg aaaaacagtt gcaacgggca actaaaatca ccgatgaaac
     1081 cggtggtggt attctggtta taaccgaagg ggtatacggg atggccggtg atctgggaaa
     1141 cctgcgcgag atcgtcgcac tcaaaaagaa ttataatttc agattactgg tcgacgatgc
     1201 acacggtttt ggaaccatgg gcaaaacagg ccagggaacc ggtgaattcc tgggtgttca
     1261 ggatgagatc gacgtttact ttgccacttt tgcgaaggct tttgccggga tcggggcatt
     1321 tatagcttcc gataaaggta ttatcaagta cttaatgtat aatctaagat cacagatata
     1381 tgccaagtcc ttgcctgttg ctatgaccat tggcgcgtta aaaaggcttg agatcatgcg
     1441 caaagaaccg cagcacagag aaagactctg gacaatcgtc aacgcactgc aaaaaggatt
     1501 aagggaaaag ggattcaaca tcggaaaaac agaatctcct gtaactcctg tctactttac
     1561 cggcggcgta agcgaagcca ctcaaataac gtacgacctg agggaaaaat acaatatatt
     1621 ctgctctatc gtggtttacc ccgttgtccc aaaagacgta atcatgctcc ggcttatacc
     1681 tacagcagtg cacacacttg aggatgtgga atatactatt agtgcctttg agaaagtcaa
     1741 acagaaactc gatgctggtg aatattccga tggaaagatc aaagccatgc aggttttctg
     1801 aaccagcacg atcttaattc tttaaagatc cctgatgtcg cctggcatat ctcataaaaa
     1861 aagaaactat tcttctgttt ataagatata caggcgactt cgctttatgc ggcaccgcaa
     1921 aaagcaattg aagcaacaga taaaacaaca gcttatccgg gaaacagatg aaaaaaaact
     1981 tgctgaagcc cggctccggg aattcaggat acaggaaaag aaacagttca aaattaaagc
     2041 caaagctgat aaaagagccg acaaagaacg gaaagccgcg ctgaaagaag aaatgaagca
     2101 aatggctata atggccaggc tggagaaaga aaaggaagta aaagaggcaa ttgaaagaag
     2161 aaaaaagcag tggcaactgg agaaactgga aacccaaagg ctaaaagaag aaaaaagact
     2221 tcagcgcata caggtgcgga ccaaattctc gtggaaggtc tattttaaaa atgcggtgat
     2281 aaaattcaag agcggcaaag cgaaacgtcg gcataatgcg ttgattacaa tcaactccac
     2341 cgtctttttt atcatgtcgt acctggcgtt gtttttcatt tcacaggcag ttaccgtgat
     2401 tgccgccggt tttttcaatt atcccgccac ggtatattac tttgagatat actttaatat
     2461 aagccctgaa gcctggtacc acgattcggt gaaaaccatt ttttcagcag ggcctcttgt
     2521 caatttcatt ctgggtgtta tttgcctgat catctaccat aaacttaaag aatctccggt
     2581 tacatttaaa atcttctttt tatggggatt tctgcacggc gttaatatgc tcttcggggc
     2641 gatgctcgtg ggtactttgt ttgaaactgg tgtcggacac gtgatcagct ggatgtatat
     2701 aatggataca ggacggctat tgtattccat cctttcaatt ttcctgctgg tgattgccgg
     2761 gatgattgca acaaaaccat tcctgttttc cgccaactcc tactttaatg aaattaatac
     2821 cggaaacagg ctggccttca ttctttcaca ggtgttctgg ccctttctga ccggaaatat
     2881 cataatgatc ctggtccgcc agccccgcct ggttttttac gacacgttta tactctttac
     2941 cctggtcatt tctgttttgg ctgtgctatt tactttttca ggccataatg aactttattt
     3001 cgaagaagaa gagaaaaagc cggggattgc atggatagca accggtattc tgatactggc
     3061 cctgattttt ttcagggtgg ttttagggat tgggatcagg ctgggataga tatttatcct
     3121 atcgcattac ggcaattttc ttgaacccaa atccctggtt cgtaaagaac ccggcgaagt
     3181 aaatgccatt tttcagggaa tgtatatcca gaaatccttc attaattggt tctgttattt
     3241 gtattaacag tttacccgag gtatcataaa tgaagacact ccgaatattc agatctgttt
     3301 tgaaattcag ataaccgtta gtcacaggga ccggatatat atcaattcgg ttactcacac
     3361 ggggattatt gattcctccc actccttccg ggaccagttt aacatccagg atcgttgcct
     3421 gcctgtttac aatctcgaca ttttgaatga ctttggtttc gtaaccgggc gccgaatagc
     3481 gaattgtgta tgtgccttcg tgcagaagcc tgtgataatt ccctgcaggc aggctggaat
     3541 agacccacga gctgtcgatc tcatgcagca cagcatagac ttcggctctt atcggccagc
     3601 cggtcacaga atccctgacc gtcccccgca gtccaaacat cgattgttcc aaataattga
     3661 gcaacgacct gtaattatat tcccaccagg ctggcaataa tcccgggcta agcagtttct
     3721 ggtccgatat ctcgagggtg aattctcggg cctgatggaa ataattcata tagtcctgcc
     3781 ttcctccgga aatggtgtac cattgatatc catttgtaat accatcatcg aaatcagaca
     3841 tataaccagc cgggctatgc aattgtgcgg tgtcggcata ttcatggcaa acatattgcc
     3901 accagctatc atcagcagca agacggctcc atgtatccca gggataatta caaacttctt
     3961 ctccgccatg tatatttgct gatgagacaa agtggttatt ttgtgccagg ttcatgaaat
     4021 ggaccgtttc tgtctgccag ggatacccgt caggatgagg cccgtcttcc ggatccggga
     4081 aattccggtt caggtctatc ccattgtaat tataccgtct ggctccgtta acggtgttat
     4141 tccccccaaa ataggtacca tcaggattgg ctaacgggtt gatccagatt tcaatttcat
     4201 tcacgagatt cgtcagacgc ggatcaatat tataattgga caaaagataa tcggccagcc
     4261 ggagcattaa gatgtagcct gtcgtctcat ctccatggat tgaagaagtg tacaaaaact
     4321 gtggttcgtt ttcactcagc ccgacattgt cggatatctt gactgccagg atttgccttc
     4381 cctggatggt ggtcccgatg gtaaccagtt gacatattga aggatattgg tcagcaaact
     4441 ggttcatcat gtcaatatag gcatcgtagg taggatagaa atcccattcg gaaatctcgc
     4501 ggatgtcaac cttttctttc atgttcagat atccggagaa atcgccggga tgaggcaaca
     4561 aatcaaatga caaacccgtt tccagaaatt tgctgaatcc ttccctgttg gcgtaagcaa
     4621 aaactcgtcc gttgttgtct actttatcaa tagatataaa tttaccgagg tactccaaac
     4681 cttgagcttg ccggaggtca aatccgaagt attgttctat ttcattatta taaaggctgt
     4741 caaggaatga tttactgtcg gaattttgac tgaacgtgct gatattgaat gcaaacaaca
     4801 gcagaataag aacggtgttg taaaatctga acttgttcat cgggtgaata ataaaaaatc
     4861 aatctatagt tctacctggt ttttgaccag aaaatcggta tgttcggcaa tcatagaatg
     4921 cacgttcttg ttaaccttgg caagaggtaa ccttacattg cttttgcaaa gacctagtaa
     4981 ttccatggct gctttaattc ctgacgggtt accatccata aacagtgcat tggtaaatcc
     5041 caataaatta taatggaggg ccctggcttt ggtaaatttt cctctcaggc acagattgac
     5101 catttcggtc atttgttttg ggaaggcatt ggcgacaacg gaaatcacgc catcggcacc
     5161 cagcgagatc aatggaaggg tcaggccatc atctcctgaa atgacgtaaa aaccagatgg
     5221 cttgttgttg atgatctcca tacattgcat aaggttgcct gaagcttcct tgataccgat
     5281 gatattggtg aagtcggcag ccaatttaag tgtggtttca gcagtgatgt taacgctggt
     5341 tcttcccggg acattgtata gtatgacggg aacctggctg gcaccggata tagctttata
     5401 atggttgtaa agacctttct gttgcggttt gttataataa gggcaaacgg ataaaatagc
     5461 ctctattcct tcaaaattgg tggctttgat cgtattgatc acctcctggg tattgttacc
     5521 gccaattccg gcgacgacag gcacccgttt gttcactgta tcgatgacaa aatccagcac
     5581 agcaactttt tcgtcttttg acagagcagc tgattctccg gtggttccaa gtgcaacgat
     5641 gtaattaata ccacctgtta taaggtgttc tatgattttc tcaaatgaac cgaagtcgat
     5701 atttccggaa tcatggaagg gtgtgataac cgcaactccg gttcctttta ggtaatcata
     5761 cattgccctt tgaatttaaa attgataaat attcattgat aatgccgaga aaagatatct
     5821 ggttatgaat gggttccgac tttagcataa tgtcgtagat ctgtgataac gccttaaaat
     5881 tatcatcggc ggctacattg ttatatgctc cggctttaag tttggctttc gacattcccg
     5941 cgatatattt caaagggaaa atatcttccg aagcaatatt gatacagatg tcaaaatctg
     6001 tttcgataaa gtctgtaaca cattttgctt ttgggatcaa aagccagttc agatctttac
     6061 cactgataaa atcgaatgat aagaccggca gaaactggct ggtggcattt tttgtcccgg
     6121 aatatcccag ggctttgact tttattttat tttcctgcag tttctggaaa aaatcctgaa
     6181 tggcataata taccgaatga tcggtgagtt cgaataaaag accgacagaa cctgctgtat
     6241 tcaggctgac cagtttttta ttccttttta gcgatgtaat ccctttttgg agaatataac
     6301 cggctattct gagtcttata ttgttaaaca aagccatgat ctcaagatga agagatgtaa
     6361 aagtagcaag attttgatca ggtacatcag ccagccgtca ttcttcctga atttttttaa
     6421 cacttttcaa attcagcata ttcaggggat ttgtgccaag attttgaata taagtccatg
     6481 aaaatcttac gacctgatcg taataaagaa tgatcacagg cgcatcttcc atcactaaac
     6541 cgttcatttg ccggtaaata tcctttctta tcaccggatc cccgaccagc tgtgaagctt
     6601 tatagagaga atcaaatacc ggattgttgt atcgggtata attggggccg gccggagcag
     6661 cgttcgtgct gacaaacaag gagagataat tctcttcgtc aggataatcg gcaatccatg
     6721 aggccctgaa aaaattgatc ctggaatagg cttttagttg cataagcgtt gcggccggat
     6781 ttacttcaat ttttacggat atacccaaac tgcttaattg atgctgcata tatttgaata
     6841 tatcggtata ttctgccgta gtatggaggg tgattgccgg aagaggattt gccgggccgt
     6901 aaccggcttc acggattaaa gcctgtgctt tttgcggatc gtagtcgaaa aatacctcac
     6961 ttgagtcaaa tgccggcaga ccgggaggta taatcccatg aagccccggg acgccaatgt
     7021 tgttcctgat gtaccggatc atctttcgcc ggtcgatgcc ataattaatg gcctgcctga
     7081 cctttttatt catcaacgga ctgttccgtc cggcatctgt aagactgtcg accagcattc
     7141 ccacatactc agtgttgaga aagggttccg tcatcatcat aaacttgtct tgatatccgg
     7201 cttttaagtg tccgtctttg gttaaaagtt catctttata agactgatcg agaccggaaa
     7261 tgaaatccag gttgcctttg ataaactcca ggaaggcggc ctgtttgtca atcagaaatg
     7321 atatggctat ggcatcaaga taaggtaacc tttgttgatt tgaatccttt tcaaaataac
     7381 cggtgttctt taccaaaacc agtttgacat tttctttcca cattttaaaa cggaaagggc
     7441 cggttcctgt cgggtgtatt ctgaattcaa tccctttttc agcaacgact tcttttggta
     7501 caacgctgca ataaatcatg gtcaggatgc cgggaaacgg tgaaaatggt tctttgagcc
     7561 ttatttgcag gatcgtatca tttaaagcgg ctatagccgg ttctccatct tccttttcaa
     7621 gtttgttcag tacccagatg ccgggagagg ccagctttgg atcctgcaat cgcttcaggc
     7681 tatatacaaa atcatgcgca gtcactttcc tgccaattcc gccgggaaat gcagggtgat
     7741 cgtggaaaaa aacatcgttt cgcagaaaaa aagtatagat ccggccggtt gtatctatat
     7801 tccaggagtg agcgatacaa ggaaggatat tgagatcttc atccagttgc aacagcccgt
     7861 tgtagatttg gttacatgcc cagatacttg cctgatcttt tgcaaaagcc ggatcgagtg
     7921 aactgatatt agagaattca ttatacctga ataccgtcaa gccggactca tccggctttt
     7981 gttccgcgca agaggaaatc attgccaata acagaccata aaatgccagt cttctcatct
     8041 ccaggtattt tatggcaatg ataagaaaaa agtcggcccc tgagaccttt acaattatca
     8101 ggatgaaaat gtctcaggct ttatcctgaa gtccagcgga ttttttattt tatcaggcag
     8161 tagggcatgt gcgatgacat caatcatttt atcgatataa atgaaattta acccttcaac
     8221 gtattctttt ttgatctcct caatatcttt cctgttctcc tgggaaagga tgatgttttt
     8281 aatttttgcc cttttggcag ccaggatctt ttccttgatt cctcctaccg ggagaacctt
     8341 tccccgtaag gtaatttcac ctgtcatggc cagttcgggc ctgatttttc gctgcgtaaa
     8401 cgccgaagcc agggcggtta gcatcgtaat cccggccgat ggtccatctt ttggcgttgc
     8461 gccttccggg acatggatgt gcacattcca gttggtaaat acatcgggat cgattcccag
     8521 gctgtcggta tgtgctttca ggtattcaaa agcaatggca gcggattcct tcatgacatc
     8581 ccccaggttt cctgtaaggg taaggtttcc cttccctctg ctgaggctga cttcaataaa
     8641 tagtatctcc cctcctgcgg cagtccacgc caggcctgtc accaccccgg gtacatctat
     8701 ccgggaatcg cgatctttct ggtacctggg cgctcccatg atcgtctcaa tatcctggtt
     8761 ggataatttt ggagtgtaac cttcttccat ggccacaagg cgggccctgt tacggactat
     8821 tttcgcaatc accttctcca aggaccggac gcccgattcc cttgtataat catcgatgat
     8881 cttttcaatg gtctttttcg ggaatgccac atgcttttct ttcaatccgt gttccctgat
     8941 ttgttttggg atcaggtgtt tgcgggcaat ttcaactttt tcttccagaa gatagccgct
     9001 gaggtcgatt acttccatcc tgtcgcggag tgccggatga atagtcgaca tattattagc
     9061 ggtggcaatg aacatcactc tcgagagatc gaagttgatc tccaggtagt tatcatagaa
     9121 ttcgaagttc tgttccgggt ccagtatctc aagcaaggct gcctgtggat ctccctggaa
     9181 attgttccca ctgactttat ccacctcatc aaggacaaat accgggttcg ccgaccctgc
     9241 ttttctaagg ctttgaatga tcctgcccgg catggcaccg atatatgttt tcctgtgccc
     9301 tctgagttcg gcctcgtccc tgagcccccc taaagagacc cgaatgtagt ttcgtcccaa
     9361 tgcccgggca atcgattttc ccagggatgt tttaccgaca cccggggggc cgacaaaaca
     9421 taaaatgggc gatttcatgt catttttcag ctttaagacc gccagatgtt ctatgatcct
     9481 ttcttttatc ttttccagac cgtaatggtc ttcatccaaa atgttctggg cctttttcag
     9541 gttgaaatca tcgtttgtga attcgttcca gggcagctcg acaacggttt ccaggtaatt
     9601 catctggatc gaatactccg ccgcctgcgg attcatccgt tgcaactttt tcaactcttt
     9661 gtcaaaaatc tccgcaatct tctttgtcca tttctttttc gccgctttgg ctttcaggtc
     9721 attgatttcc tgttcatgcg gactaccgcc aagctcttcc tggattgttt ttaattgctg
     9781 gttcagcagg taatcgcgtt gttgtttgtc aagatcgagc tttactttat cctggatctg
     9841 gtttttcaac tcaagcatat gcagctccct ggccatggct gtcaggacct cattcgcccg
     9901 cttggtaaga tcgggcgttt ccagaagttt ttgtttatcc accaggtcga tgttcaggtt
     9961 ggaggataca aaattgatca ggaaaaaagg gctgtcgata tttttaatgg cgaatgcggc
    10021 ttcggaaggt atgttcggcg aaagcttgat tatctggata gcgaggtctt tcagggatcc
    10081 gatcagggcc agaaagtgct catcctgttc cagattttta tcgaagtcgg tcaggtcctt
    10141 gatttttgct ctgaaatagg gttcgttttg aataaaacct tcaatttcaa atcttttttt
    10201 tccttgaatg atgactgttg tgtttccgtc gggcatctgc aggattttaa taagatgggc
    10261 gacggtgccc acattataca agtctttttc tcccgggtcc tcaatggccg aatccttttg
    10321 tgccaccacg ccgatggtcc ggtcattacg gtaggattcc cggatcaggc ggatggattt
    10381 atcacgcccg actgtaatgg gaataaccac accgggaaaa aggactgtat ttcgaagggg
    10441 cagaatcggg agggattcgg gcagcttttc cgaatgcatt aactcttctt cttctgacga
    10501 taaaagagga atgaattccg tatcgttatc aaccgcgtcg gaaaggccat ttatattaaa
    10561 gagatcatcc ataaaacgag tgtcaaattg tcagtagtat tttcggtatg caaatccggt
    10621 gtgccctttc tctgcttcaa acatggatat aacctgatgg ttgccgggaa attgcccagc
    10681 cgtatttcaa ctactatgcc agaatcaaag gttcggtgag gctatggcaa cggttccttg
    10741 gttaaattat aaacatcgcg caggatatct tcttctgtct gatcgaattc gagaccgcgc
    10801 cgctggtata ctttcctggc cacggacacg gcgcggtcga tgtggtagga tttgattccg
    10861 gaaggccctc cccaggaaaa actgggtata aagttcctct ggtaacccga tccgaatatg
    10921 ttggaattga tgccgacaac cgttccggta ttaaacattg tattaatgcc gcatttggag
    10981 tggtcgccca tgatcaggcc gcagaactgc agcccggttt caacaaaagt ctggtcggca
    11041 taattccaca gcttaattgt ttcgtaagta tttttaaggt tggatgtatt ggtgtcagcg
    11101 ccaatattgc accattcgcc gatcaccgat tgtcccatga aaccatcatg agccttgttg
    11161 gagaatccga aaaacacgct gttgttgatc tcccctccca ctttgcagtg aggtcctatc
    11221 gtagtagggc cgtatatttt cgcacccatt ttaacggtag catgcgcccc caggccgaaa
    11281 gggcctctga tcaggctgcc ttccatgatt tccgcatctt caccgatgta tataggaccg
    11341 gttgatgaat tcagtatgca gcattccacc tttgcattct tctctataaa tacctcattt
    11401 ccaattacct gattggattt gctgacaggg cctgaaaccc tgccgtgcgt gagtaaattg
    11461 aaatcctcac gtattgccct gtcattttgt gaaaatatat cccacgtatg ggtaattttc
    11521 aaaatgtcgc ttttggattc gaccatatta acatcgtcaa tatgctcatc atcaagcttc
    11581 tcaagttcaa cggcatgcaa cctcatggca atgattgcat ctccatggaa cagtacctcg
    11641 ccagttttca ggcagaggat ctcctccagc agttgcttat tcgggcagac tgacccgttg
    11701 atcatgatgt tgtcctcttc cctgacaaca gggaatttct tacggagata gctttctgtt
    11761 aaagtggagg ttttctggcc caggtaccgt tcccattttt cccggatggt cagaatccct
    11821 gtgcggatat cagctaccgg acggagatat gtcaggggga aaaggttgct tctgcgaaaa
    11881 ttatcaaata aaataaaatt catatcttct ttttatttca aaaataaaac aaaaaaagcc
    11941 ctcagcgcag gcaaagggct tttatactag tgtgaaaaaa gtgtctattc agatgcgctt
    12001 tctctctttt ccaggtgctg tttgtacctg ttcttgaatt tatcaacacg cccggccgta
    12061 tcaaccagtt tcatcttacc ggtgaaatag ggatgggatg tattcgatat ttcaagtttg
    12121 atcagcggat attcttttcc atcttcccat gtagccgttt cttttgactt agccgtcgac
    12181 ttggtaagga atgaataatt attcgacatg tccttgaaaa tgacaaaacg gtaatctttc
    12241 gggtgaatgt ttttcttcat gatcttattc catttcaaat tcgggctgca aaggtagtta
    12301 ttttattgta ataaccaaat ccgataccga aaaaacttaa aaattataag ctaaacagga
    12361 attccattgt atcgatgcca tcggcataat cccataattc aggcatctgg ctttttccga
    12421 aaggtactgc ccctttgaat gctttacctc ctgccaccac gcattgcaga ctgtcctgct
    12481 gttctctgag aatgagatca aggctgtcaa ccgaccggta aggttcggta
//
LOCUS       DAOPNH010000063        12421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5303_length_12421_cov_6.514394, whole genome shotgun sequence.
ACCESSION   DAOPNH010000063 DAOPNH010000000
VERSION     DAOPNH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5303_length_12421_cov_6.514394"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1568
                     /locus_tag="PKZ74_04895"
     CDS             264..1568
                     /locus_tag="PKZ74_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92129.1"
                     /translation="MLEHIKEIILDAQSGPLFTGTPRQTGLACIPGKASIIIGVRRSG
                     KSTVLNQEVDKWLSKGVLKENIFYINFFDDRLSGLKTIGLDIVLEAYYSLYPGKKNLE
                     TVYCFFDEIQVIPEWESFIDRILRTENVVVYLSGSSAKMLSKEMATQMRGRALAWELF
                     PFSFKEYTGHQNLKNDLPLNTQQRLFIENAFGEYWETGGFPEVAGMEKHLRIKIHQEY
                     YNSILFRDLIDRYDIPHPRALNDLSRHLLANVASMYSINSLTGMLKSLGYGVPKSVIA
                     EYISWFEDAYFLFTVRLYDASYRRSNANSKKIYCIDHALVRSITPGILTNSGHLLENI
                     VFLALRRVTAEIWYYRTTGNREVDFIYRNKIGEPVLIQVCESILIPGTRDREIKALRA
                     AMQELRITKGYIVTRNETEQITYPQGIIAVMPAWKFLLESWE"
     gene            complement(1608..2231)
                     /locus_tag="PKZ74_04900"
     CDS             complement(1608..2231)
                     /locus_tag="PKZ74_04900"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HPM92130.1"
                     /translation="MKTLSIILVLLINGISSFSQSQIITAKDLPKLAAMMTGQFSSEE
                     QAAADKDFLHIRLSMTPIMPENKDGYWLYVEQAMASAMDKPYRQRVYRLSIADDTTIV
                     SKVFELKNPLAYTGGHEMPEKLAVITPDSLIDRQGCSIFLHKTGKKRFEGSTPGKECL
                     SSLRGATYATSEVVITPKQMVSWDRGWDREDKQVWGSEKGGYVFLKR"
     gene            2493..3935
                     /gene="sufB"
                     /locus_tag="PKZ74_04905"
     CDS             2493..3935
                     /gene="sufB"
                     /locus_tag="PKZ74_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPM92131.1"
                     /translation="MPDTNEILDNLTKSEYKYGFVSDIDTETAPVGLDENTIRYISSR
                     KEEPEWMLEYRLKAYRHWLTMKEPEWAHISYPPINYQDIIYYAAPKKKQFTSLDEVDP
                     ELLATFDKLGIALEEQKRLTGVAVDAVIDSVSVKTTFQETLAKHGIIFCSFSEAVKNH
                     PDLVKKYMGSVVPYTDNYFAALNSAVFSDGSFCYIPKGVRSPIELSTYFRINAANTGQ
                     FERTLIVAEEGGYVSYMEGCTAPMRDENQLHAAVVEIMAMDNAEVKYSTVQNWYPGDK
                     EGKGGIYNFVTKRGLCKGANSKISWTQVETGSAITWKYPSCILMGENSIGEFYSVAVT
                     NNRQQADTGTKMIHIGKNTKSTIISKGISAGFSNNSYRGQVRITARAENARNFSQCDS
                     LLLGDKCGAHTFPYIDVDNRTAIVEHEATTSKISEDQLFYCQQRGIDMEKAISLIVNG
                     YAKEVLNHLPMEFAVEAQKLLSISLEGSVG"
     gene            4018..4773
                     /gene="sufC"
                     /locus_tag="PKZ74_04910"
     CDS             4018..4773
                     /gene="sufC"
                     /locus_tag="PKZ74_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPM92132.1"
                     /translation="MLLQLKELHVSIKGKEIIHGLNLEVKAGEVHAIMGPNGTGKSTL
                     ALAIAGKEGYEITAGEVIFNGLVINEMPPEERARLGIFLGFQYPVEIPGVSLTNFMRT
                     AINEVREYRGLPPMPAGEFLKMMEEKKQLVEIQSKLTNRSVNEGFSGGEKKKNEIFQM
                     AMLEPMLAVLDETDSGLDIDALKIVANGVNMLRRPDNATIVITHYQRLLTYIVPDFVH
                     VIYNGKIVRSGGKELALELEEKGYDWIKKELNV"
     gene            4840..6252
                     /gene="sufD"
                     /locus_tag="PKZ74_04915"
     CDS             4840..6252
                     /gene="sufD"
                     /locus_tag="PKZ74_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPM92133.1"
                     /translation="MKTTLHNGSFKENLLSLYEGNEEYIARNDSAYSSTIRSKGFVHF
                     QNLNLPDRKNELWKNTDLSEVLKNDYIKYLNRAETGQDLNFMFTCEIHNFKTDQVSFL
                     NGWHIKAGSSLPDLPAGVIICSISEAFRFYPDLAEKHYGRYAEAGKDIFLAMNTAFTQ
                     DGLFVYVPDNIILEKPVQMISIINHNENLLIQNRNLVILGKNSRMTLVMCDDSTNQQA
                     SFNNSVTEVFLDEGSHFEHYKLQNLNNSSTLLNSTWFYQEAGSRLDTYAITLNGGLIR
                     NYSNVKLNGSGADAHINGLYLVDRKQHVDNRVFVEHAVADCTSKELFKGILDDEASAV
                     FNGHVLVKKDAQRTNAYQQNRNILLTDKATVNTRPFLEIYADDVKCSHGATVGQLDSE
                     ALFYLRSRGICMASARLLLMYAFAAEVISGMTLQALKDRIDEMIRQRLRGELHICQTC
                     VLHCANQDKEIHFDIDMSKI"
     gene            6338..7558
                     /locus_tag="PKZ74_04920"
     CDS             6338..7558
                     /locus_tag="PKZ74_04920"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPM92134.1"
                     /translation="MSFDILHIRHDFPILDVTVHDKQLIYFDNAATTHKPRQVVDRIV
                     AYYKKENCNIHRGVHYLSTMATGAYENARKTVKDFINAEHPHEVIFTKGATEAINLVA
                     QTFGRKNIHQGDEIILSEMEHHSNLVPWQMLAEQKGAVLKVIPVDEQGELEVQHLKSM
                     ITHRTKLVALAHVSNVLGTINPVHRVITLAHEQGIPVLLDGAQAVPHLEVDVKELNCD
                     FYAFSAHKLYGPMGVGVLYGKEKYLEEMPPYQGGGEMVDQVTFQKTTYNKLPYKFEAG
                     TPNVSGVLGFEAALKYILDLDLKKIHAYERELLDYTTLELLKMDAVKIFGNAENKTAV
                     ISYNIGKIHPYDAGTIYDKLGIAVRTGHHCAQPLIDRFGVPGMIRASLGLYNTIEEID
                     RFIEATRQVRRMLA"
     gene            7737..8126
                     /locus_tag="PKZ74_04925"
     CDS             7737..8126
                     /locus_tag="PKZ74_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM92135.1"
                     /translation="MGAFTSFEEMEVWKKSIHLTRMVYQITIQPAFAKDYYLVNQIRK
                     SAVSIASNIAEGFERDGNREFINFLYIAKGSCGELRCQLYIALELQYMTQESFQEIYN
                     FAKEISVSINKLIKYLQSSDYKGIKYK"
     gene            8302..8721
                     /locus_tag="PKZ74_04930"
     CDS             8302..8721
                     /locus_tag="PKZ74_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPM92136.1"
                     /translation="MNITEIEANIVAEFSAFDDWMDRYNYLIELGKDLKVIDEKYKVQ
                     NNLISGCQSRVWLHAEFRDGKIYFSADSDAVITKGLISLMIRVLDGQTPDDILSAELG
                     FLDQIGLKEHLSPTRSNGLTSMIKQMKLYALAFKTKS"
     gene            8914..9240
                     /locus_tag="PKZ74_04935"
     CDS             8914..9240
                     /locus_tag="PKZ74_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HPM92137.1"
                     /translation="MTDINIVKKDLEEKIIAALKEVYDPEIPVNIYEIGLIYKIRIDD
                     DFLVHIVMTLTTPNCPVAESLPEEVKQKVLAIKGVNDVDLELTFEPPWDMEMLSDEAK
                     LELGIL"
     gene            9363..11174
                     /locus_tag="PKZ74_04940"
     CDS             9363..11174
                     /locus_tag="PKZ74_04940"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPM92138.1"
                     /translation="MNLHAVPGPILSSINSPADLKKLPEEQLPLLCKEIRAFLIEETS
                     QNPGHLGSSLGAVELAVAIHYVFETPYDKLIWDVGHQAYAHKILTGRRDLFSTNRTLG
                     GISGFPKMSESEYDAFGTGHSSTSLSAALGMAVASALQGDPERMHISVIGDAALAGGM
                     AMEALNHAGVFKANLLVVLNDNGISIDPNVGAFKDYLARIADGQPVLHTRSNFFEAFN
                     FQYFGPVDGHDVIQLVNRLREIRYIPGPKLLHTLTTKGKGFRKAEEEQTVYHAPGYFN
                     KVTGELIEKESAGGIPPKYQDVFGRTIIELARQNEKIVGITPAMPTGSSLVMMMAEMP
                     DRAFDVGIAEQHAVTFAAGMAARGMIPFCNIYSSFMQRAYDQVIHDVALQKLPVVFCL
                     DRSGLVGEDGATHHGAFDLAYFRAIPGLTICSPMNEEELRNMMFTAQLPGAGPTVIRY
                     PRGRGAMVDWQKPFRELIRGTGRRIREGNDVAILSIGHIGNEAEKAAAMLEKDNISVA
                     HYDMRFLKPPDEALLHEVFRNFSKIITVEDGTITGGLGSAVLEFMADHQYNAQVTRLG
                     IPDRFIEQGTVGELQRECGFDANGIIRSSKFKVQSSK"
     gene            11243..12031
                     /locus_tag="PKZ74_04945"
     CDS             11243..12031
                     /locus_tag="PKZ74_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPM92139.1"
                     /translation="MNWIEIAVLIFSGLAVGFINTLAGGGSVISLSVLMLMGLPAPVA
                     NGTNRIAVTIQTLTAATVFKKKKVLDLKKGTILGIPSAIGSLLGAYIAVDINEATFEK
                     AFAVIMLIMLVFVFYNPDKYIYGKQHLVDKKVTPLQWLIFFVIGIYGGFVHVGIGYFL
                     LAALVLNAGYDLVRANAVKVLIVLMYMPFSFLVFLWMSDINWTYGLVMMIGSVAGAYI
                     ASHLAVNKGIVFVKWSIVVVTVFMAGHFLGIYDIKEMFGVMLGK"
     gene            12236..>12421
                     /locus_tag="PKZ74_04950"
     CDS             12236..>12421
                     /locus_tag="PKZ74_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92140.1"
                     /translation="MKYLIFLLIPFLVVACSIKNADRPDYVLVIHGGAGVMEKKDFAP
                     ELEKEYLDKLQQALDTGE"
BASE COUNT         3334 a         3007 c         3149 g         2931 t
ORIGIN      
        1 aaaaaaaata aaatcaggat aaattttttc atggttggta gtttttggtt tgattggaag
       61 taagattggt taaaaataca ataaatttca aaacaccata ttattttttc ttaaagaaga
      121 tatatccgcc tttctccgat ccccatacct gcttgtcttc cctgtcccag gtgattatta
      181 tttaacttat atttgtaatc ggtaattatt attactactt ttgctacaaa gtagatataa
      241 tattttcctg tttgatgatt gccatgttag aacatataaa ggaaatcatt ctggatgccc
      301 aatcaggtcc gttatttaca ggaacgccga ggcaaaccgg gttggcttgc ataccaggca
      361 aagcttctat tattatcggg gtcaggcggt ctggcaaatc tacagttcta aaccaggaag
      421 ttgacaaatg gttgtcaaaa ggtgtattga aagaaaatat tttctacatc aatttttttg
      481 acgaccggct atcggggctg aaaaccattg gcctggatat tgttctggaa gcctattatt
      541 cgctttatcc cggtaaaaag aacctggaga ccgtatactg tttttttgat gagatacagg
      601 taatccccga atgggagtca ttcattgaca ggatattaag aactgagaac gtggttgttt
      661 acctttccgg ttcatcagca aaaatgctat cgaaagaaat ggcgacacaa atgcggggaa
      721 gggcgctggc ctgggagctc tttccctttt cattcaagga atacacgggg catcagaatc
      781 tgaaaaatga tcttccgctg aacacacaac aacggctttt catcgaaaat gcatttggtg
      841 agtactggga aaccgggggc tttcctgaag tggcaggaat ggaaaagcat ctccggatta
      901 agatccacca ggaatactat aattccatcc tttttcgcga tctcatcgac cgatacgaca
      961 ttccgcatcc cagggcacta aacgaccttt cccgccattt gctggcaaat gtcgcatcca
     1021 tgtatagcat caacagtttg acagggatgt taaaatcact gggatacgga gtgccaaaga
     1081 gtgtcattgc ggaatacata agctggtttg aagatgccta ttttcttttc acagtaagat
     1141 tatatgatgc ttcgtaccgt cgttcgaatg caaattcaaa aaagatatat tgcatcgacc
     1201 atgctctggt gagatcaata acaccgggga ttttgaccaa cagtggccac ttactggaaa
     1261 atattgtctt ccttgcgctc agaagagtaa ccgccgagat atggtattac aggacgaccg
     1321 gtaaccggga agtggatttt atatatcgga ataagattgg tgaaccggtg ctgatccagg
     1381 tgtgcgaatc gattttgatt cccggcaccc gcgacaggga aataaaggca ctgagggctg
     1441 ccatgcaaga gctcagaatt acgaaaggat atattgtcac gcggaatgaa acggaacaga
     1501 ttacataccc gcagggaatc atcgcagtta tgccagcctg gaagtttctg ctcgaatcat
     1561 gggaatagtt ataatttcat atcctggatt cacatttgcc cggtgcacta tctcttgagg
     1621 aagacatatc cgcctttctc cgatccccag acctgcttgt cttccctgtc ccagccgcgg
     1681 tcccaggaaa ccatttgttt aggcgtgata acgacttctg aagtggcata ggttgcgccc
     1741 cgcagcgaac tcaggcactc tttgcccggg gtggaacctt caaagcgttt tttccccgtt
     1801 ttgtgcagga aaatgctgca tccctgccgg tcgatcagtg aatccggggt aataacagcc
     1861 agtttctccg gcatctcatg tccgccggtg taggccaacg gattcttcag ctcaaagact
     1921 ttgctgacaa tcgtcgtgtc gtccgcaatg ctcaggcggt agacccgctg gcggtagggt
     1981 ttatccatcg ccgaggccat cgcctgttcg acgtaaagcc agtagccatc cttgttctca
     2041 ggcatgatgg gggtcatgct cagccggata tggaggaagt ccttatctgc ggcggcctgc
     2101 tcttcgctgc tgaactgccc ggtcatcatg gcggcgagct tcggaaggtc tttggcggtg
     2161 ataatttgtg actgggagaa ggatgaaatc ccgtttatta ataacaccag gatgatacta
     2221 agcgttttca tagacttatt tttatagttt acaaaactat gtttttaagc ttatcaatag
     2281 cacacggatg acacggatgc gacggattaa cgcggattta tttcggattt tctattcact
     2341 atgcacgagg gcgaaagaat tgatgaaacc tggaacctgg aacattggaa ccctggaaca
     2401 attctccccc ttctttgttt catatgttac agtccaatcc gatgagaatg gttacttttg
     2461 caatctcaat ttagattaaa tcactgaagg cgatgccgga tacgaatgaa atactcgaca
     2521 atctgaccaa aagcgaatac aaatacggat tcgtatcgga tatcgatacg gagacggcgc
     2581 cggtcggact ggacgagaac acaatacgat atatctcttc gcgcaaggaa gaaccggagt
     2641 ggatgctcga ataccgcctg aaagcttacc gccactggct gacgatgaag gagccggagt
     2701 gggcgcatat ctcctacccg ccaatcaatt accaggatat catttactac gccgcgccga
     2761 aaaagaagca gttcaccagc ctcgatgagg tcgatcccga actgctggcc actttcgaca
     2821 aactggggat tgccctggaa gagcagaaaa gactgacggg cgtagccgtg gatgcggtga
     2881 tcgacagcgt atcggtaaag acgaccttcc aggagacgct ggcaaagcac gggatcatct
     2941 tttgctcgtt cagcgaagcg gtgaagaacc accccgacct ggtcaaaaaa tacatgggct
     3001 cggtagtgcc ctacaccgac aattactttg cggctttgaa ctcggccgtt ttcagcgacg
     3061 gctcattctg ctacatccct aaaggtgtcc gcagcccgat cgaactgtcg acctacttcc
     3121 gcatcaacgc cgccaatacc ggccagttcg aaaggaccct catagtggct gaagaaggcg
     3181 gctatgtcag ttatatggaa ggctgcaccg ccccgatgcg ggatgagaac cagctccatg
     3241 ccgccgtggt ggagatcatg gcgatggaca atgcagaggt gaaatattcg acggtccaga
     3301 actggtaccc gggcgacaag gaagggaaag gaggcattta caactttgtg accaaacgcg
     3361 gtctttgcaa aggcgccaac tcgaagatat cctggaccca ggtggagacc ggctcggcga
     3421 tcacctggaa atacccgagc tgcatcctga tgggcgagaa cagcatcggc gaattttact
     3481 cggtagccgt taccaacaac cggcagcagg ccgatacggg gacgaagatg atccatatcg
     3541 ggaagaacac caaaagcacg atcatttcca aggggatctc ggccgggttc agcaacaaca
     3601 gctaccgcgg ccaggtgagg ataacggcga gggcggaaaa tgccaggaac ttctcacagt
     3661 gcgattcgct tctgctgggc gacaaatgcg gggcgcacac gtttccctac atcgatgtgg
     3721 ataaccgcac tgccatcgtc gaacatgagg caaccacgtc gaaaatatcg gaagaccagc
     3781 ttttctactg ccagcagcgg ggtatcgaca tggaaaaggc tatcagcctg atcgtgaacg
     3841 gctatgctaa agaggtgctg aaccatctgc cgatggaatt cgcggtggaa gcgcagaaac
     3901 tgctgagcat atcgcttgaa gggagcgtgg gataagaagc agccccctcc cttactccct
     3961 cccccaattg ggggagggac ggggtgggag ctaactttga actttgaact aaatataatg
     4021 ttactacaac taaaagaact gcacgtttcg ataaagggaa aagagataat tcacgggttg
     4081 aacctggagg tcaaggccgg ggaggtacat gccatcatgg ggccgaacgg aacgggaaaa
     4141 agcacgctgg ctttagctat tgccggcaaa gaaggatacg agatcaccgc cggggaggtc
     4201 atctttaacg ggctggtcat caacgaaatg ccgcctgaag aacgggccag gctgggtatc
     4261 tttctcggtt tccagtaccc ggtggagatc ccgggcgtca gcctgaccaa cttcatgcgg
     4321 acagccatca acgaagtgcg cgaataccgc gggcttcctc cgatgccggc cggggaattc
     4381 ctgaaaatga tggaagagaa gaaacaactg gtcgagatcc agtcgaagct gaccaaccgc
     4441 tcggtcaacg aaggtttctc aggcggtgag aagaaaaaga acgagatctt ccagatggca
     4501 atgctggaac caatgttggc tgtgctcgat gaaaccgatt cgggactcga tatcgatgcg
     4561 ctgaagatcg tggctaacgg cgtcaatatg ctccggcggc ccgacaatgc aacgatcgtc
     4621 atcacccatt accagcggct gctgacctac atcgtccccg attttgtaca tgtgatctac
     4681 aacggcaaga tcgtccgttc gggcggaaaa gagctggcgc tcgagctgga agaaaaggga
     4741 tatgactgga ttaaaaaaga attaaatgtc tgaataaccc caccccctgc ccctcccctg
     4801 atatcagggg aggggttgga gaggggtcca taaaatataa tgaaaacaac actgcataac
     4861 ggatcgttca aggaaaacct tctcagcctg tatgaaggaa atgaagaata tattgcccgg
     4921 aatgattcag cctattcatc aactatccgc tcaaagggtt tcgttcattt tcaaaatctg
     4981 aaccttcccg accggaaaaa tgaactctgg aaaaacaccg acctttctga agtccttaag
     5041 aatgactaca ttaagtatct gaaccgtgcc gaaacgggac aggacctgaa tttcatgttc
     5101 acctgcgaga tccataattt caaaaccgac caggtctctt tccttaacgg atggcatata
     5161 aaagcgggaa gctcactgcc cgaccttccg gccggggtga tcatatgcag tatcagcgaa
     5221 gcattcaggt tttacccgga tttggcagag aagcattacg gccgttacgc cgaagccggc
     5281 aaggatattt tcctggccat gaataccgct ttcacacagg acgggctgtt tgtctatgtc
     5341 cccgacaaca tcatactgga gaagcccgtc cagatgatca gcatcatcaa tcacaacgaa
     5401 aatctcctca ttcaaaaccg caacctggtc attctgggca aaaacagccg gatgacgctg
     5461 gtcatgtgcg atgactcaac caaccagcaa gccagtttca acaactcggt cacggaggtc
     5521 tttctggatg aaggatctca tttcgagcat tataagctgc aaaatctgaa taatagctcg
     5581 accttgctga actcgacgtg gttttaccag gaagccggga gccggctcga cacgtatgcc
     5641 attactttga acggcggatt gatccgcaac tattcgaatg tgaagcttaa cggcagcggc
     5701 gccgatgcgc atatcaacgg cctttacctg gtcgaccgga agcagcacgt tgataaccgg
     5761 gtatttgtcg aacacgctgt tgctgattgc accagtaaag aacttttcaa aggcatcctc
     5821 gacgatgagg cttcggccgt gttcaacggg catgtcctcg ttaaaaaaga cgcccagcgg
     5881 accaatgcct accagcagaa ccggaacatc ctgcttacgg ataaagcgac ggttaatacc
     5941 cggccgttcc tcgagatcta tgccgacgac gtcaaatgca gccatggagc caccgttggg
     6001 cagctcgaca gcgaagcgct gttttacctt cgttcgcgtg gcatatgcat ggccagtgcc
     6061 cgtttgctgc tgatgtacgc ttttgctgcc gaggtgatct ctggcatgac gctgcaggcg
     6121 ttgaaggacc gcatcgatga aatgatccgc cagcggctgc gtggtgagct ccacatctgc
     6181 cagacgtgtg ttctgcattg tgccaaccag gataaggaaa tccatttcga tatcgatatg
     6241 agtaaaatct gatgatcctt ttacttatca acagcccacg gttttcggaa caattttact
     6301 tcaattcaac taattttgcc gccaaaataa aacaaaggtg agtttcgaca ttctgcatat
     6361 acggcacgat tttcccatcc tcgatgtgac ggttcacgat aaacagctga tttatttcga
     6421 taatgcggca actacccaca aaccccgcca ggtcgtcgac cggatagtgg cctattacaa
     6481 gaaagagaac tgtaatatcc accggggagt acattacctg agcaccatgg ctaccggggc
     6541 ctatgaaaat gcccgaaaaa cggtaaaaga ttttatcaac gccgaacatc cgcatgaagt
     6601 gatctttacc aaaggggcga cggaggcgat caaccttgtg gcccagactt tcggcagaaa
     6661 aaatattcac cagggcgatg agatcatcct ttcggagatg gagcatcatt ccaacctggt
     6721 accctggcag atgctggccg agcaaaaagg ggcggtattg aaagttatcc cggtcgatga
     6781 gcagggcgaa ctggaagtgc agcacctgaa atcgatgatc acccaccgga cgaaactggt
     6841 cgccctggcc cacgtttcca acgtcctggg caccattaac ccggtccacc gggtgattac
     6901 gctggcccac gagcagggca taccggtcct tctcgacggc gcgcaggctg tgccgcacct
     6961 cgaagtcgat gtcaaggagc tgaactgcga cttttacgct ttctcggcac acaaactcta
     7021 cggcccgatg ggcgtcgggg tgctttacgg aaaagaaaaa tacctggagg agatgcctcc
     7081 gtaccagggt ggcggtgaga tggtcgacca ggtgacattt cagaaaacga cgtacaacaa
     7141 gcttccgtac aaattcgagg ccggcacgcc caacgtttcc ggcgtcctgg gattcgaggc
     7201 cgctcttaaa tacatcctcg acctcgacct caagaaaatt cacgcttacg aaagggaatt
     7261 gctcgattac acgacactgg agctgcttaa aatggatgcg gtgaagattt tcggcaatgc
     7321 tgaaaataaa acggcagtca tttcttacaa tatcggcaaa atacatccct acgacgccgg
     7381 cacgatctac gacaaactcg gcatcgccgt ccgcaccggg catcactgcg cccagccgct
     7441 gatcgaccga ttcggtgttc ccggcatgat ccgcgcctcc ctggggcttt acaacaccat
     7501 cgaagagatc gaccggttca tcgaagcgac caggcaggtg agacgaatgc tagcttaagg
     7561 ttgcgggttt ccaaagttcc agtgttccat tgttccaaag ttccagtgtt ccagtgttcc
     7621 aaagttccag cgttccaaag ttccagagtt catatgtgag actgtttaga ttaaagttat
     7681 caacaaccgg gtatttttta ctaaaaagcc actctaatag gataaataat ttcagtatgg
     7741 gtgcttttac aagttttgaa gagatggagg tctggaaaaa atctatccat cttacacgaa
     7801 tggtttatca aattaccatt caacctgctt ttgcaaaaga ctattacctg gtgaatcaga
     7861 taaggaagtc tgctgtatca attgcttcaa atattgcaga agggtttgaa agagatggaa
     7921 atagagaatt tatcaatttt ctttacattg ccaaaggatc gtgtggagaa ttgcgttgtc
     7981 aactttatat tgctttagaa cttcaatata tgacccaaga aagctttcag gaaatatata
     8041 attttgcaaa agaaatcagc gtatcaataa ataaattgat aaaatacttg cagagttctg
     8101 attataaggg tattaaatat aaataaactt gcccgaacta aacagacttt aaaacattgg
     8161 aacactggaa ctttggaaca ctggaacact ggaacactgg aacattggaa cactggaaca
     8221 ttggaacact ggaacactgg aacactggaa ctttggaaca atggaacact ggaactttgg
     8281 aacccggaac cctggaacaa catgaacata accgagatcg aagcaaacat cgtagccgag
     8341 ttcagcgctt tcgacgactg gatggaccgg tacaattacc tgatcgaact ggggaaagac
     8401 ctcaaggtga tcgatgagaa gtataaggtt cagaacaacc tgatctccgg gtgccagtcg
     8461 agggtgtggc tgcacgcaga attccgcgac ggaaagatat atttctctgc cgacagcgat
     8521 gccgtgatca ccaaaggatt gatcagcctg atgatccggg tgctcgacgg gcaaacgccg
     8581 gatgatatcc tcagcgcgga gcttggcttt ctggatcaga tcgggttgaa ggagcacctc
     8641 tcccccaccc gctccaacgg gctgacctcg atgataaagc aaatgaaact ctatgcactt
     8701 gcgtttaaaa ctaagtcata atgaatgtat ttcgtgctcc aacgggtaaa cccgttggct
     8761 atgggtacta ttttataacc gatgggttta cccgtcggag caggaacgat tgcagcccga
     8821 cgactgaagc cgtcggttca gggaggatta aaaaatcata accgatgggt ttaccttcgg
     8881 actacaaata gaccaggtta agcagaaaaa accatgacag atataaatat tgtaaaaaaa
     8941 gacctcgaag aaaagatcat tgccgctctg aaagaggtct atgatcctga gatccccgtg
     9001 aatatatacg aaatcggatt gatctacaag atccggatcg atgacgactt tctcgttcat
     9061 atcgttatga ccctcaccac acccaactgc cccgttgcgg aaagccttcc ggaagaggta
     9121 aagcagaagg tactggcaat taagggggta aatgacgtag atcttgaact gactttcgaa
     9181 ccgccctggg acatggagat gctcagtgat gaggcaaagc tggaactggg gatactttag
     9241 ttggacagtt ggacagcatg tcctgagggc gaagcccgaa ggatcgcagt tagacagttg
     9301 gacagtaacc cttacaacct ttaccagcta aattactacc tttgaacttt gaactttgaa
     9361 ctttgaactt gcacgctgtt cccggtccta tattgtcttc cataaactct ccggcagatc
     9421 ttaaaaagct tccggaagag cagttgcctc tgctttgcaa agaaatacgc gcatttctta
     9481 ttgaagagac ctcccagaat ccggggcacc tcggttccag cttaggcgca gtggaactgg
     9541 cggtagccat ccactatgta tttgaaacgc cgtacgacaa gctgatctgg gacgtgggtc
     9601 accaggccta tgcccacaaa atattaaccg gaaggcgtga tcttttcagc acaaaccgaa
     9661 cacttggcgg gatcagcgga ttcccgaaaa tgagcgaaag cgaatacgat gctttcggaa
     9721 cgggccattc ctcaacctct ttatcggctg cgctgggtat ggctgttgcc tccgcgttac
     9781 agggcgatcc ggaacggatg cacatttccg tgatcggcga tgcggcgctg gccggaggga
     9841 tggcaatgga agcgctgaac catgccggcg tgttcaaagc gaacctgctg gtggttttga
     9901 acgataacgg catttcgatc gatccgaatg tcggtgcatt taaagactac ctggcccgga
     9961 tcgcggatgg gcagcctgtc ctgcatacgc gcagcaattt cttcgaagcg ttcaactttc
    10021 agtacttcgg cccggttgat ggccatgatg tgatccagct cgtgaacagg ctgcgggaga
    10081 tcaggtacat tccggggcca aagctgctcc acaccctgac gacaaagggc aaaggattcc
    10141 ggaaagccga agaggagcaa accgtttatc atgcgcccgg atatttcaat aaggtcaccg
    10201 gggaactgat agaaaaagaa tcggccgggg gaatcccgcc gaagtaccag gacgttttcg
    10261 gccgcacaat cattgaatta gccaggcaga acgaaaagat cgtagggatc acgccagcca
    10321 tgcccaccgg ctcttcgctg gtgatgatga tggccgagat gcccgaccgg gcctttgacg
    10381 tggggatcgc cgagcagcac gccgtaacat tcgcagccgg catggccgcc cgggggatga
    10441 tcccgttctg caacatttat tcaagcttca tgcagcgggc ctacgaccag gtgatccacg
    10501 acgtggcatt gcaaaagctg ccggtggtat tctgcctcga ccgcagcggg ctcgtcggtg
    10561 aagacggtgc cacgcaccat ggcgctttcg atcttgcgta tttccgggcc ataccgggcc
    10621 tgaccatctg ctcccccatg aacgaggagg agttgcgcaa catgatgttt actgcccagc
    10681 tgccgggcgc cgggccgacg gtgatccgct atccgcgggg ccggggggca atggtcgact
    10741 ggcagaaacc attccgggaa ctgatccgcg gcacaggccg gcgaatccgc gaagggaatg
    10801 atgtggcgat tctgtccatt ggccatatcg gcaatgaagc agaaaaagcc gcagccatgc
    10861 ttgaaaaaga taacatctct gtggcgcatt acgacatgag attcctgaag ccgccggacg
    10921 aagcgctgtt acacgaagtg ttcagaaatt tcagcaagat catcactgtc gaagacggga
    10981 ccatcaccgg cggactgggc agtgccgtgc tggaattcat ggcagatcat caatacaatg
    11041 cgcaggtgac gaggctcggc atccccgacc ggttcatcga acagggaacg gttggcgaat
    11101 tgcagcggga gtgcgggttt gatgccaatg gaatcattcg tagttcaaag ttcaaagttc
    11161 aaagttcaaa gtgagtcggg cagctaccac ccttaccacc cctattcaga aattgatacc
    11221 tttgcacttt gaactttgaa ctttgaactg gatagaaatt gccgtactga tcttctccgg
    11281 cctggcggtc ggattcatta acacgctggc gggcggcgga tcggtgatct ccctgtcggt
    11341 gctgatgctg atgggattgc ctgcgccggt ggccaacggc accaaccgga tcgccgttac
    11401 cattcagacc ttgacggccg ccaccgtttt caaaaagaaa aaagtcctcg atttaaaaaa
    11461 agggacgatt ctcgggattc cctccgcaat cggctcttta ctcggtgctt acattgcggt
    11521 ggatatcaat gaggccacct ttgaaaaagc tttcgccgtg atcatgctga tcatgctggt
    11581 ttttgtattt tataatccgg ataaatatat ttacggcaaa caacaccttg tcgataaaaa
    11641 agtaaccccg ctgcagtggc tgatattctt tgtaataggt atttacggtg gtttcgtcca
    11701 tgtcgggata ggttatttcc tcctggccgc ccttgtgctc aacgccggct acgacctggt
    11761 gcgtgccaat gcagtaaagg tgctgatcgt attgatgtac atgccattct ctttcctcgt
    11821 cttcctctgg atgagcgata tcaactggac ctacggcctg gtgatgatga tcggatcggt
    11881 ggcaggggct tatattgcct cgcacctggc tgtaaataaa gggatcgtgt tcgtcaaatg
    11941 gtcgatcgtg gtagtcaccg tttttatggc cgggcatttc ctggggatct acgatattaa
    12001 ggagatgttc ggggtgatgt tggggaagtg agtcattggc tcccttcggt cgcgtcattt
    12061 actcgctgcg ctcgtgtcat aagctcactg cgttcacgtc atttactcgc tgcgctcgtg
    12121 tcataaggtt gttagaagtt gtatttttat caattgaatc ggtgacccgt aaattaaaaa
    12181 catccgtgtt aatccgtatt ttccgtgtca tccgcgtgcc attaaaaaga tcactatgaa
    12241 atacctgatt ttccttttaa ttccttttct cgtcgttgca tgttcgataa aaaatgctga
    12301 tcgtcctgat tatgtcctgg tcatacacgg gggtgcaggt gtaatggaaa agaaagactt
    12361 tgccccggag ctggaaaaag aatatctcga caaactgcag caagcgctcg ataccggcga
    12421 a
//
LOCUS       DAOPNH010000064        12253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5399_length_12253_cov_5.304640, whole genome shotgun sequence.
ACCESSION   DAOPNH010000064 DAOPNH010000000
VERSION     DAOPNH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5399_length_12253_cov_5.304640"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /locus_tag="PKZ74_04955"
     CDS             <1..2128
                     /locus_tag="PKZ74_04955"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPM92141.1"
                     /translation="LDKNVLVMLTGLNVGDRVTIPGEDISGAIKKLWDQGLFEDIGLY
                     ITDIQGETVFLNLKLKERPRLSRFSLRGINKTEADNLREKLNITRGDVVTESLLKNTR
                     HRIREHFRDKGFLDAEIDIRQNPDTTRANNVVLDIFIKKGNRVRIDEIRIFGNNEFSA
                     QKIKNSLKETKERGKFNPLQDVELALLHNIREILRMNPQGVVDSTMAHFNRTVKIRIF
                     KPSRFIQDKYEEDLVKVIEKYNKVGYRDASIISDSIYRNADRTLSIDLKMAEGRKYYF
                     RNITWVGNTKYTADQLNRVLKIDRGDVYNRELLETNLSFNPNEQDVSSLYLDDGYLFF
                     SAEPVEINVENDSIDIEIRIREGKQARINKVAISGNTKTSDHVVMRELRTRPGQLFSR
                     SDIIRTTRELAQLRYFDPEKITPDIVPNPADGTVDITYKVEETSSDQIELSGGWGYGR
                     IIGTLGLSFNNFSARKLFKSDAWKPVPSGDGQKLSLRLQSYGKGYLSYSASFTEPWLG
                     GKKPNAFSVTYWHSLYSNGKSKNDPLRSEFVIDGLTLGLGRRLTWPDDFFTLYQSVTG
                     QRYALENYSQIFVFGSGTGVYNNINYNIVLGRSSISQPIYATYGSDVSVSLEVTPPYS
                     LFSKDDYSVMEPDERYKWIEYHKWKVNATFFTEIFPKAVIRPGLSLDSLDSITGISGS
                     RRLKGSIWGAMDCRGIITLMAGRSSE"
     gene            2044..2472
                     /locus_tag="PKZ74_04960"
     CDS             2044..2472
                     /locus_tag="PKZ74_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM92142.1"
                     /translation="MTPFERFYLGGDGLSGYNNLDGREIIGMRGYRNETITPDYYKSK
                     NIGGTIYAKYTLELRYPLSLNPSATIYAMAFLEGGNSWLGFDDFNPFSIYRSAGFGVR
                     VFLPMFGLLGLDWGYGFDDVPGLPSANGSQFHFSINSSID"
     gene            2540..3271
                     /locus_tag="PKZ74_04965"
     CDS             2540..3271
                     /locus_tag="PKZ74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM92143.1"
                     /translation="MKKLILISALLMMAFMPGFSQKYAFVDTDYILNNIPEYTDAQAL
                     LDDLSAEWQKEIEAKFQEIDKLYREYQAESILLPEDLKKQKENEIVQKEKDAKDLQKK
                     RFGKDGDLFKKRIELVQPIQEKIYNAIQDMAVTRNYAFIFDKASGAALLFADAKFDLS
                     DDVLDEVGTVMQTVKRENRKRQTVELGGTGTGTGTGKGSDLSPGQMVPQARPGQGTNV
                     NPTERPGSEGIKDNPMPPPGEKKPR"
     gene            3330..3845
                     /locus_tag="PKZ74_04970"
     CDS             3330..3845
                     /locus_tag="PKZ74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM92144.1"
                     /translation="MKKILLFVMVIALSTVMSAGFGQVNAKLGYIDSNELLELMPGKD
                     SIQTALQDYQKTLETQLQTMYAEYQTKVQDYQTNSRTMSDIIRQTKEKELADLETRIQ
                     TFQQQADTDLQNKQVELLQPLLDKAKNAINAVAKENGYTYIFDVGTGAFLFYEKGDNI
                     LPLVKAKLGLK"
     gene            complement(3908..4423)
                     /locus_tag="PKZ74_04975"
     CDS             complement(3908..4423)
                     /locus_tag="PKZ74_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPM92145.1"
                     /translation="MALIKTVKGIIPNIGKRCFLADTATIIGDVILGNDSSVWFNAII
                     RGDVHYIRIGHHVNVQDGVIIHCTYRQAPVTIGNNVSIAHNAIVHGCTIHDNVLIGMG
                     SILMDRVIVHSNSIIAAGALVTKGTVVEPDSVYAGAPAKKIKSIDKSLSEGEIQRIAN
                     SYNMYAGWYKL"
     gene            complement(4434..6044)
                     /gene="nadB"
                     /locus_tag="PKZ74_04980"
     CDS             complement(4434..6044)
                     /gene="nadB"
                     /locus_tag="PKZ74_04980"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPM92146.1"
                     /translation="MRKTIDFLVIGSGIAGLSYALKVADKGTVCIITKARADETATKY
                     AQGGIAAVMYTPDTYAKHILDTMRAGDDLSNPEIVKIAITESTERVKELMDWGVDFDK
                     KESGRFDLHKEGGHSEFRVLHHKDQTGAEIERALLSKVKDHPKIEMLENHFTVDLITQ
                     HHLGSEVNRRTPGITCYGAYVLNPDTGEIHTLLSKITLLATGGAGQVYANTTNPVIAT
                     GDGIAMVYRAKGIIENMEFIQFHPTTLYNPDETPSFLITEALRGFGAILKTKAGTEFM
                     HKYDVRGSLAPRDIVARAIDNEMKITGDDYVCLDCRHLDEKELIHHFPTIYAKCLSLG
                     INIAKDMIPVVPGAHYTCGGIKVDEYARSSIINLYATGECASTGLHGANRLASNSLLE
                     SAVFSHRAALDSREKADHTAINTNVPDWNAEGMILNEEMVLITQSLKEVQSIMSSYVG
                     IVRSDLRLKRAFDRLRIIYRETEDLYNKSILTVKLCELRNLINIGYLIIRMALERKES
                     RGLHYSIDYPHAKAKESIDHQENGLADY"
     gene            complement(6087..7382)
                     /gene="odhB"
                     /locus_tag="PKZ74_04985"
     CDS             complement(6087..7382)
                     /gene="odhB"
                     /locus_tag="PKZ74_04985"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPM92147.1"
                     /translation="MIIEVKVPSPGESISEVQLAGWLVEDGEYVEKDQDIAEIDSDKA
                     TLSISAAESGMIRILKAKGENVGVGEVVATIDTDGKQAPARQKKEVIPAKPVQKIEIE
                     GVTAQDKPPVAAEITEEKQEEAKLHLSPLAKKLLDANNLSQAEFIEYLKQVRFGKSDI
                     EFFIKEKEKNKDHISDSPLVIPSKPSKDSRETERRQMSTLRLKLAERLVSVKNETAML
                     TTFNEVNMGQIMKIRQQYKESFKEKYGISIGFMSFFTKAVTEALQLFPQVNARIDGDE
                     MVFHHFADIGIAVSTPKGLMVPVLRNAENMSIADIELNIKELATKARDGKISLDEMKG
                     GTFTITNGGVFGSMLSTPIINPPQSAILGMHNIVERPIAVNGQVEIHPIMYVALSYDH
                     RIIDGRESVSFLVKVKEMLENPARMLFGGNDPVGRLLGL"
     gene            complement(7392..10250)
                     /locus_tag="PKZ74_04990"
     CDS             complement(7392..10250)
                     /locus_tag="PKZ74_04990"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPM92148.1"
                     /translation="MDRFSYLNANDPAMIEALYEKFQADPASVDEGWRQFFEGFEFAR
                     KHYPVRKQEGIAVYPSEFKVINLINAYRQRGHLFTQTNPVRTRRKYSPTLDIENFGLN
                     NEDLSKSFEAGHEIGIGKATLKEITDHLKETYCQSVGAEFFYIRNPEVKTWLQDKMER
                     SRNSYPFTLDDRKYIYRDLARAVLFEKFIHKKFPGQKRFSLEGAEALIPALDAIMEKG
                     ALLGTEEFIIGMPHRGRLNVLANILRKPYSHIFSEFRGMKYEDETLLGDVKYHLGYTI
                     ERYTTAGKKMKLTLAPNPSHLEAVDPVVEGIVRARADMDYKGDFNRIAPILIHGDASV
                     AGQGIVYEVIQMSQLPGYKTGGTIHVVVNNQIGFTTNYLDARSSTYCTDVAKVVQSPV
                     FHVNGDDVEAIVATIQLAMEFRAKFQRDVFLDILCYRKYGHNEGDEPRFTQPILYKII
                     ETHPNPMEIYRRQLIEKGFLEASYFDEVEAKIHETLEKDLEESRKIDKANIRSFLDNV
                     WKDIRKATPADFDTSPETGVDLKKIKSIGRRIAHVPEGENYFRKLVRLQESRLEMIEK
                     TGQVDWAMGELLAYGTLLEEGFPVRISGQDSIRGTFSHRHAVLKIEDSEKEYYPLAHI
                     SDRQARFEVYNSPLSEYGVLGFDYGYSLASPQTLTIWEAQFGDFANTAQVIFDQFICS
                     AEDKWNVMNDLVILLPHGYEGQGPEHSSARIERFLILSAEYNMQVANVTTPANFFHLI
                     RRQLHREFRKPLIVFTPKSLLRHARCISPLTDLASGSFREVIDDPDADQANIKKVVFC
                     TGKVYYDLLEEKEKMKYNDTAIIRIEQLHPFPYKQIRNILDKYLHTDDILWTQEEPAN
                     MGAWTFIQTKMQEFNLRLIARPASGSPATGSPEFHELQQRKVIEKTFSSCDCPKVAEE
                     CRMMCIGNRWKTFEEQIMKTGQHISSKSVSAINKLQNK"
     gene            complement(10349..10516)
                     /locus_tag="PKZ74_04995"
     CDS             complement(10349..10516)
                     /locus_tag="PKZ74_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM92149.1"
                     /translation="MAYKITDDCTACGTCIDECPVEAISEGDIYVIDPDICTDCGSCA
                     DVCPVDAIEPA"
     gene            10804..>12253
                     /locus_tag="PKZ74_05000"
     CDS             10804..>12253
                     /locus_tag="PKZ74_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPM92150.1"
                     /translation="MKDNKNHKIIELEHVVVKLAGDSGDGMQLSGSQFSDTTAFDGHD
                     LATFPDYPAEIRAPQGTVAGVSGFQLHFGHIKVETPGDLADVLIALNPAALKVNMKWV
                     KPGATIIVDVDSFEPKDIEKAGWQNNPLDDGTLDGYRVIKAPITSLSRTTCAEIGLDA
                     KSADRTKNQFALGILYFMFHKDVELGAKFLQEKFAKKELVAAANKAVLEAGYHFAENL
                     QEFDITYRIKPSDDMKGRYRSINGNTAAAWGLMAAAEKAGLELFLGSYPITPATEILQ
                     ELAHHKELGVKTLQAEDEIAGISSAIGAAFAGKLAATSTSGPGLALKSEAIGLAVITE
                     LPLVIIDVQRGGPSTGLPTKPEQSDLMQALFGRNGESPVVVMAASTPGNCFDYAFMAA
                     KIAVEHMVPVILLTDGYLANGSELWKVPKMADLPSIKPRRVDDNDPDYKPYRRDPQTL
                     ARSWAVPGQEGLRHRIGGLEKEDVIGIVSHDPL"
BASE COUNT         3119 a         3010 c         2905 g         3219 t
ORIGIN      
        1 tctcgataaa aacgtgttgg tcatgctcac cggtctcaat gtcggtgacc gtgtcaccat
       61 ccccggggaa gacatctcag gtgctatcaa gaaactatgg gatcagggcc tgtttgaaga
      121 tatcggattg tatatcaccg atatacaggg ggaaacagtc tttctgaacc tgaaactgaa
      181 agaaagacca cggctttccc gtttttcact gagagggatc aataaaacag aagctgacaa
      241 cctgcgcgaa aagctcaata tcaccagagg ggatgtggtt acggaaagcc ttctcaaaaa
      301 tacgcgccac cggatcaggg aacatttccg ggataaggga tttttggatg cggagatcga
      361 catcaggcaa aaccccgata caacacgtgc caataatgtt gtccttgata tatttattaa
      421 gaagggaaat cgtgtcagga tcgatgaaat ccggatcttc ggaaacaatg aattttcagc
      481 ccagaagatc aaaaattcgc tgaaagagac caaggagaga gggaaattta atccgctgca
      541 ggatgttgaa ctggcccttt tgcacaatat ccgggaaatc ctcaggatga atccgcaggg
      601 agtggtcgat tcgacaatgg cccacttcaa ccgcacggtg aaaatcagga ttttcaaacc
      661 ctccaggttt atccaggaca aatatgagga agacctggtc aaggtcattg agaagtataa
      721 caaagtcgga taccgtgacg cctccatcat cagcgattcc atttaccgca atgcggaccg
      781 gactctcagt atcgacctga aaatggcaga ggggaggaag tactatttca ggaatattac
      841 ctgggtcgga aatacgaaat acacagccga ccagctgaac agggtactaa agattgacag
      901 gggagacgtg tacaaccggg aattgctgga aacaaaccta tcattcaacc cgaatgaaca
      961 ggatgtcagt tcgctatacc tggatgacgg gtatttgttc ttctccgccg agccggttga
     1021 gatcaatgtt gagaatgatt cgatcgacat cgagatcagg atacgggaag gcaaacaggc
     1081 acggatcaac aaggtggcaa tcagcggaaa taccaagacc agcgaccatg tcgtgatgcg
     1141 cgagctgcgt acccgcccgg gccagctatt cagccgctcc gatataatcc gcaccacccg
     1201 cgaacttgca caacttagat atttcgaccc ggagaaaata accccggata tcgtccccaa
     1261 cccggcagac ggaaccgtcg atattaccta taaagttgaa gaaacttctt ccgaccagat
     1321 cgaactcagc ggcggatggg gatatggccg tatcataggt acccttggcc tctctttcaa
     1381 taatttttcc gcccggaaat tattcaaaag cgatgcctgg aaaccagtcc cttcgggcga
     1441 tggtcagaaa ttaagcctcc ggctgcagtc gtatggaaaa gggtacctgt cgtacagtgc
     1501 ttcttttacc gaaccatggc tgggaggcaa gaaaccaaac gctttcagtg taacgtattg
     1561 gcattcactt tattcaaacg gaaaatccaa gaatgacccg ctccggtcgg aatttgtgat
     1621 cgatggattg acgctcgggc tgggacgaag acttacatgg ccggatgact tctttacctt
     1681 ataccaatcg gtaaccggtc agcgttatgc gttggaaaac tattcccaga tatttgtgtt
     1741 tggatcgggc acaggcgttt acaataatat caactataac attgtccttg gaagaagttc
     1801 catcagccag cccatctacg caacatatgg ttcggatgtc tctgtatccc ttgaagttac
     1861 accgccttat tcgcttttta gcaaggacga ctattcggtc atggaaccgg atgaaagata
     1921 caagtggatc gaataccata aatggaaggt gaatgccaca ttcttcaccg agatatttcc
     1981 caaagcagtc atcagacccg ggctaagctt ggattccttg gactctataa cagggatatc
     2041 gggatcacgc cgtttgaaag gttctatctg gggggcgatg gattgtcggg gtataataac
     2101 cttgatggca gggagatcat cggaatgagg ggataccgga atgaaaccat cactcccgac
     2161 tattacaaga gcaaaaatat cggcgggact atctatgcca aatatacgct ggaactaaga
     2221 tacccgctat cgctcaatcc cagcgcaacg atctatgcca tggcattcct tgaaggaggt
     2281 aactcatggc ttggatttga tgattttaat cctttctcca tataccgcag tgcaggattt
     2341 ggggtcaggg tcttcctgcc gatgttcggc ctcctggggc ttgactgggg atatggattt
     2401 gatgatgtac ccgggctccc gtcagccaac ggctcccagt tccacttctc gatcaactcg
     2461 tcaattgatt aaccgaagct aatatggcag gttttttgct ggtaatcctt ttatcattaa
     2521 atatattgga ggaataaata tgaaaaagct cattttaatc tcagcgcttc tgatgatggc
     2581 ctttatgccc ggttttagtc agaaatatgc attcgtcgat accgattata tcctgaataa
     2641 catacctgag tacaccgatg cccaggcttt gctggatgac ctctctgccg aatggcagaa
     2701 ggaaatcgaa gcgaaattcc aggagatcga taagctttac agggaatacc aggcagaatc
     2761 gatcctgttg cccgaagatc tgaaaaagca gaaagagaac gagatcgttc agaaggaaaa
     2821 agatgccaaa gatcttcaga aaaaacgctt cggtaaagac ggtgacctct tcaaaaaaag
     2881 gatagaactg gttcagccca tccaggagaa aatctacaac gcaatccagg atatggccgt
     2941 gacacggaat tatgccttta ttttcgacaa agcatccggg gcagcattgt tgtttgccga
     3001 tgctaagttc gatctcagcg acgacgtcct tgatgaagtc ggtaccgtga tgcagaccgt
     3061 taaaagggag aaccgtaaac gtcagaccgt tgaactggga ggtaccggta cgggtaccgg
     3121 aaccggaaaa ggctccgatc tttcacccgg gcagatggta ccccaggcac ggcccggaca
     3181 gggaacaaat gtcaatccga cagaaaggcc tggttctgag ggtataaaag ataaccccat
     3241 gccgccgccg ggtgaaaaaa aaccaaggta ataggaaatt cataaatttc catatctttg
     3301 ccgcacttaa ttaattaatc atactgaaaa tgaaaaagat cttattattt gtcatggtga
     3361 ttgccctcag cactgttatg tccgccggat ttggacaggt gaacgccaaa ctgggctata
     3421 ttgattcaaa tgaattattg gagttaatgc cgggaaaaga ctccattcaa acggcactgc
     3481 aggattacca gaaaacgctg gaaacccagt tacaaaccat gtatgccgag taccagacaa
     3541 aagttcagga ctatcagaca aattcccgca ccatgtcaga tattattcgc cagacaaaag
     3601 aaaaagaact ggcagatctt gaaacccgta ttcagacttt ccagcaacaa gccgataccg
     3661 atttgcagaa caaacaggtg gaacttctgc aaccgctgct ggataaagca aaaaatgcca
     3721 ttaacgccgt cgctaaagag aatggatata cctatatttt tgatgtcggt accggcgctt
     3781 tcctttttta tgaaaaagga gacaatatcc tgccgctcgt caaagccaaa ctgggattga
     3841 aataaatttc cactgatcaa ataaaagagc ctgactcaaa atcctgagca ggctcttttt
     3901 tattttttta caacttgtac catccggcgt acatgttata gctgttggca atccgttgta
     3961 tttccccttc cgataaactt ttgtcgatgg atttgatttt tttggcaggg gctcctgcat
     4021 aaacgctgtc gggttccacg accgtccctt tcgttacgag cgctcctgcc gcaattatgc
     4081 tgttggaatg aacaatgacc cggtccataa ggatggatcc catgccgatc aggacattat
     4141 catggattgt acagccatgt actatcgcat tgtgtgcaat agatacgtta ttccctatgg
     4201 tgacgggcgc ctggcggtag gtacagtgta tgatcacacc atcctggaca tttacatggt
     4261 gcccgatccg gatgtaatga acatcgcccc gtatgatcgc attgaaccac acactgctat
     4321 catttcccag gataacatcc ccgataatgg ttgccgtatc ggccaggaaa caccgtttcc
     4381 cgatattcgg aatgattcct tttactgttt ttatcagtgc catgacagaa cggttaataa
     4441 tcggcaaggc cattttcctg gtggtctatc gactctttcg ctttggcatg cggataatcg
     4501 atcgaatagt gcaaaccacg gctctctttc ctctccagtg ccattctgat aataagataa
     4561 ccgatattga tcaggttgcg gagttcgcac agtttcaccg ttaaaatgga tttattgtag
     4621 aggtcttcgg tttcacggta aatgatgcgc aaccggtcga atgccctctt taaacgcagg
     4681 tcggatctga cgatcccgac atagctcgac atgatggact gcacttcttt taagctttgt
     4741 gtgatgagca ccatctcttc gttcagtatc atcccttctg cattccagtc aggaacattt
     4801 gtattgattg ctgtatgatc cgctttttcc cttgaatcga gcgctgcccg gtgcgaaaag
     4861 acagccgatt cgaggaggga gttggaagcg aggcggttgg ccccgtgcaa acccgtcgat
     4921 gcacattcgc cggtagcata gaggtttatg atactgctgc gcgcgtattc atccactttg
     4981 atgccgccgc aggtataatg tgcgcccggc actacgggaa tcatatcttt ggcgatattg
     5041 atgcctaaac tgaggcattt ggcataaatg gtcggaaaat gatggatcag ctccttttca
     5101 tccaggtgac ggcaatccag gcaaacataa tcatcccctg tgatcttcat ttcgttgtcg
     5161 atcgcccggg ccacgatatc ccgcggtgcc agcgaacccc tcacgtcata cttatgcatg
     5221 aattcggttc ctgccttggt cttcagaatg gcgccaaagc cccggagcgc ctccgtgatc
     5281 agaaacgaag gtgtttcatc cggattatat aatgtggtag gatgaaactg aataaactcc
     5341 atattttcaa tgatcccctt cgcccggtac accatggcaa taccatcgcc ggttgcaata
     5401 accgggttgg tggtattggc atacacctgt ccggctccgc ccgtcgccag caaggttatc
     5461 ttcgacaaca aggtgtgtat ctcacctgta tccggattca gcacataagc gccgtagcac
     5521 gtaatgcccg gtgtcctccg gtttacctca gaacccaggt ggtgctgggt aatcaggtcg
     5581 accgtgaaat gattctcgag catttcaatt ttagggtgat ccttgacctt actgagcaac
     5641 gccctttcga tctcagcccc tgtctggtct ttatggtgca gcaccctgaa ttccgaatgc
     5701 ccaccctctt tgtgaagatc gaaccttccc gattcttttt tatcgaaatc cacaccccag
     5761 tccattaact cttttacccg ttccgtcgat tcggtgatgg caattttgac gatctccgga
     5821 ttgctcaggt catcgcccgc cctcatcgta tcaaggatgt gtttggcata ggtgtcgggc
     5881 gtgtacatca ccgctgcgat tccgccctgg gcatatttgg tggctgtctc atcggccctg
     5941 gctttggtaa taatacatac cgttccttta tccgccactt tgagagcgta gctcaaaccc
     6001 gcaatgccgg acccaatcac aagaaaatct atcgtcttac gcatacctca atttttccac
     6061 tgttccactg ttccacctta ccacccttac aaccccaaca acctacccac agggtcattt
     6121 cccccgaaaa gcatcctggc cggattttca agcatctctt tcactttaac gaggaaactc
     6181 accgattccc tgccgtcgat gatccggtga tcatacgata gcgctacata catgatgggg
     6241 tgtatctcaa cctgcccgtt cacggcaata ggacgctcca cgatattatg catccccagg
     6301 atggcacttt gcggcggatt gatgatcggc gtcgaaagca tggaaccgaa tacacccccg
     6361 ttagttatgg taaatgtccc gcctttcatt tcgtcgaggc tgatcttgcc gtctcttgct
     6421 ttagtagcca gctctttgat gttcagctct atgtcggcaa tagacatatt ttccgcattt
     6481 cttaaaaccg gaaccattaa cccttttggc gtgctgactg caattccgat gtcagcaaaa
     6541 tgatggaata ccatctcatc gccatctatc cgtgcattga cctggggaaa aagctgtaac
     6601 gcttccgtga ccgcttttgt gaaaaatgac atgaacccga tagaaatgcc gtacttttcc
     6661 ttaaaggact ccttgtattg ctgcctgatc ttcatgatct gacccatgtt cacttcatta
     6721 aatgtggtca gcatggccgt ttcattttta accgaaacaa gccgttcggc cagctttaac
     6781 cggagggtgc tcatttgcct gcgttccgtc tccctggaat ctttggaggg ttttgacggg
     6841 ataaccaatg gactatcaga aatatgatct ttattttttt ctttttcttt tataaagaat
     6901 tcgatatccg atttgccgaa acgaacctgt ttcaggtatt caataaactc cgcctggcta
     6961 agattattgg cgtccagaag ctttttggcc aacggcgaca agtggagctt tgcttcttcc
     7021 tgtttttctt ccgttatttc agcagcaacg ggaggcttat cctgtgctgt caccccttcg
     7081 atctcaatct tttgaactgg tttcgcagga ataacctcct ttttttgcct ggccggggcc
     7141 tgtttgccgt ctgtgtcgat ggtggctacg acctctccga cgccaacatt ttcgcctttg
     7201 gctttcagga tgcggatcat acccgactcc gccgcgctga tactcagtgt cgctttatcg
     7261 gaatcgattt cggctatatc ctggtctttt tcaacatatt caccatcttc aaccagccag
     7321 cctgccagct ggacttcgct gatcgattca cccggactgg gcacttttac ctctatgatc
     7381 atacgctaaa cctatttgtt ttgcagtttg tttatggctg aaacggattt gcttgaaatg
     7441 tgctgacctg ttttcatgat ttgctcttca aatgttttcc agcggttgcc gatgcacatc
     7501 atccggcatt cttcagccac cttaggacaa tcgcaactgc tgaatgtttt ttcgatgact
     7561 ttgcgttgct gaagttcgtg aaattccggt gaaccggttg ccgggcttcc actggccggc
     7621 cgggctatca gtcgcaggtt gaattcctgc atttttgtct ggatgaatgt ccatgcaccc
     7681 atattggccg gctcttcctg tgtccacaaa atgtcatctg tatgaaggta tttatcaagg
     7741 atgttcctga tttgtttata agggaaagga tgcagttgtt caatccggat gatggccgta
     7801 tcattgtatt tcatcttctc tttttcttca agaagatcat aataaacttt accggtgcag
     7861 aacacgactt tcttaatatt agcctgatcg gcatccggat cgtcgataac ttcccggaag
     7921 cttcccgatg ccagatcggt cagtggggat atgcaccggg catgacgaag caggcttttg
     7981 ggtgtaaata cgataagcgg tttgcgaaat tcgcggtgta actggcgtcg gataaggtgg
     8041 aagaaattgg ccggggtcgt cacattggcg acttgcatat tgtattcggc actgaggatc
     8101 aggaaccgct cgatccgggc gctcgaatgt tcgggcccct ggccttcata accgtgcggc
     8161 agcaaaataa ccaggtcgtt catcacattc catttgtctt cggcactgca aatgaactgg
     8221 tcgaaaatga cctgggccgt gttggcaaaa tccccgaact gggcttccca aattgtgagc
     8281 gtctgaggag aagcgagcga ataaccgtaa tcgaagccaa gaacgccgta ttccgaaagg
     8341 ggcgaattgt acacctcaaa acgggcctgg cgatcactga tatgggccag gggatagtac
     8401 tctttttccg aatcttcgat ctttaaaaca gcatgacggt gcgaaaaagt cccgcggatc
     8461 gaatcctggc cgctgatacg aacagggaat ccttcttcca gtaacgtgcc ataggccagt
     8521 aattccccca tggcccagtc gacctggcct gttttttcaa tcatttccaa acggctctcc
     8581 tgcaatctga ccagcttgcg gaagtagttt tccccctccg gtacatgggc tatcctgcgg
     8641 cctatggatt ttattttctt cagatcaaca ccggtctccg gtgaagtgtc gaaatcggca
     8701 ggtgtggctt tgcggatatc cttccagaca ttgtcgagga atgaccggat attggcttta
     8761 tcgatcttcc gcgattcttc caggtccttt tccagcgtct cgtggatttt ggcctccacc
     8821 tcatcgaaat aagatgcttc caggaaccct ttttcgatca gctgccggcg atagatctcc
     8881 atcgggttgg gatgggtctc aatgatttta tataggatgg gctgtgtaaa acggggttca
     8941 tccccttcat tatgaccata tttcctgtaa cacaaaatgt cgaggaaaac gtcgcgctgg
     9001 aatttggccc ggaattccat cgccagctgg atggttgcca cgatggcctc cacatcgtcg
     9061 ccgttcacat gaaaaacagg cgactggacc actttggcca catcggtgca ataggtgctc
     9121 gaccgggcgt cgagatagtt agtcgtaaaa ccgatctgat tgttaaccac gacatggatc
     9181 gttcctccgg tcttgtatcc gggtaactgc gacatctgga tgacttcgta aacaatgccc
     9241 tgtcccgcga cagaggcatc gccatggatc aggatggggg ctatccggtt gaaatcgcct
     9301 ttgtaatcca tatctgccct ggccctgaca ataccttcaa ctaccggatc gacggcttcc
     9361 aggtgggagg gattgggtgc cagcgtcagt ttcatttttt tccctgccgt ggtatatctt
     9421 tcgatcgtat aacccagatg gtatttgaca tcacccagca aggtttcatc ttcatatttc
     9481 attccccgga attcgctgaa tatatggctg taaggtttcc ttagaatgtt ggccagtaca
     9541 ttcagcctgc ccctgtgagg catcccgatg ataaattctt cggtacccag cagggctcct
     9601 ttttccatga tggcatccaa agccggaatg agcgcttccg ctccttccag ggagaaccgc
     9661 ttttgaccgg ggaacttctt gtgaatgaat ttttcgaaca gaacggccct ggccaggtca
     9721 cggtagatgt acttcctgtc gtcgagagta aatgggtatg aattgcgtga cctttccatc
     9781 ttgtcctgca accaggtttt aacctcagga ttacggatgt aaaagaattc tgcgcctacc
     9841 gactggcaat atgtctcctt gaggtggtca gttatttctt tcagcgtggc cttaccgatg
     9901 ccaatttcgt gaccggcttc aaatgattta gagaggtctt cattattcag gccgaagttt
     9961 tcaatatcga gggtagggga gtatttccgg cgggtccgga ccgggtttgt ctgggtgaag
    10021 agatgtccgc gctggcggta ggcattgatg aggttgatca ccttgaattc ggaagggtac
    10081 acagcaatac cttcctgctt cctgaccggg taatgtttcc tcgcaaattc aaacccttcg
    10141 aaaaactgcc gccacccttc gtcgaccgag gccgggtctg cctggaattt ttcgtaaagt
    10201 gcttcgatca tggccggatc attggcattc aggtatgaaa atctatccat aaatagatat
    10261 ttagcaaaag atgttgcaaa gatacaaaat gcagggcaat aaaaaaggcc gctccgggga
    10321 gcagcctttt tctggatcgg cctgattctt atgccggttc tattgcatca accgggcaga
    10381 cgtctgcaca ggaaccacag tcggtgcaga tatccggatc aatcacatag atatcacctt
    10441 cagaaattgc ttcaaccggg cactcatcga tgcaggtgcc gcaagctgta cagtcatcag
    10501 taattttgta agccatttta ttgtgtttta aaagtttgtt gtaatttatt gaatgcaaat
    10561 gtagtttttt catatacatc ctttacggaa ttaaaggttt ttttgtattt tatacaaaat
    10621 ctaaataaat cgagaagatg ggtttaccgt aacctgatca tcattcttgt taaaggattc
    10681 acaatctaga atattgataa taaggatgat gcaactgtgt ccaattactt ttttacaatt
    10741 taatagatgt acctgctttt ccgttgttct aaaattgtaa ttttgaccat taattttttg
    10801 ttcatgaaag acaataagaa tcacaagatc attgagctgg aacacgtggt ggtaaagctg
    10861 gccggggatt ccggcgacgg tatgcagctc agcggatcac aattctctga tacgactgca
    10921 tttgatggtc atgacctggc tacttttccc gattatcctg ccgaaattcg cgcaccgcag
    10981 ggaacggttg ccggcgtatc cggtttccag cttcatttcg gccatattaa agtggaaacc
    11041 cccggcgatc tggccgatgt gctcatcgcc ctgaacccgg ccgccctgaa ggtaaacatg
    11101 aagtgggtca agcctggagc gacaattatc gttgatgtcg acagctttga accgaaagat
    11161 atcgaaaaag ccggctggca gaacaacccg ctggatgacg gaacccttga cgggtaccgg
    11221 gttatcaaag cgcctataac cagccttagc cgtacgacct gtgcggaaat aggccttgat
    11281 gccaaatcgg ccgacaggac caagaaccag ttcgccctgg ggatcctcta ttttatgttc
    11341 cataaggatg tggaactcgg cgcaaaattc ctccaggaga aatttgccaa aaaggaactg
    11401 gttgccgcag ccaacaaagc cgtactggaa gccggatacc attttgccga aaacctgcag
    11461 gagttcgaca tcacctaccg aatcaaaccc agtgatgata tgaaggggag gtaccgttcg
    11521 atcaatggta atacagcggc ggcatgggga ctgatggcag cagcagagaa ggcaggcctg
    11581 gagctcttcc tggggtctta tcccattacc ccggctaccg agatcctcca ggaactggcc
    11641 catcataaag aactgggcgt taaaaccctt caggcggaag atgaaatcgc gggcatctcc
    11701 agcgcaatag gtgctgcatt tgccggcaaa ctcgcagcca cttcaacctc tggcccgggg
    11761 cttgctctga aaagcgaagc cattggcctg gctgttatta ccgaactgcc gttggtgatc
    11821 atcgacgttc agcggggggg gccctctacc gggttgccca ccaaaccgga acaatccgac
    11881 ctgatgcagg cacttttcgg acgaaacggt gaaagcccgg tggttgtcat ggctgccagt
    11941 acgccgggca attgcttcga ttacgctttc atggcagcca aaattgcagt cgaacacatg
    12001 gtgcccgtta ttcttcttac cgacggatac ctggccaatg gatcagaact ttggaaggtg
    12061 cctaaaatgg ctgatctccc ttcaataaaa ccacgacggg tggacgacaa cgatcctgat
    12121 tacaaacctt acaggcggga tccacaaacc cttgcccgtt cctgggctgt cccggggcag
    12181 gaaggccttc gccaccggat cggggggctt gaaaaagaag atgttatcgg aatcgtatcc
    12241 catgatccgc tga
//
LOCUS       DAOPNH010000065        12206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5429_length_12206_cov_6.068801, whole genome shotgun sequence.
ACCESSION   DAOPNH010000065 DAOPNH010000000
VERSION     DAOPNH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5429_length_12206_cov_6.068801"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /gene="crtI"
                     /locus_tag="PKZ74_05005"
     CDS             complement(<1..1414)
                     /gene="crtI"
                     /locus_tag="PKZ74_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM92151.1"
                     /translation="MPGKVAVIGAGIGGIAVAVRMALKGYRVDVFEKNSYPGGKMAEL
                     RHGGYRFDTGPSLFTLPGLVSELFELAGESMDDHLPYRKLDETCRYFFRDGMVIHAFS
                     TPERFADELQRKAGEDPARVLKYLSDSRKIYELTDPVFISNSLHLKGNYLKTPFLKAF
                     LQLYRLKPFSTLHRVNRNYFSHSNTVNIFDRFATYNGSNPYLTPATLMVIPHLEHNTG
                     AYFPEKGIYGIASALTELAGKKGVKFHYNSKVDEILVKNGRISGIRVSGQGEIEYDMV
                     ISDADIWFIYRHLLKQKPFPEKWFCHERSSSALIFYWGMKTQSENLGLHNILFAKDYK
                     EEFDCLFNKKVIHDDPTVYLFISSKMVKDDAPGGCENWFVMINTPENCGQDWDSMITA
                     ARAKIGEKILRMTGIDAGRYRQFEFVMDPRGIEEKTASYRGSLYGNSSNSMFAAFRRH
                     PNFSGIKGLYMVGGSVHPGGG"
     gene            1487..2971
                     /gene="crtI"
                     /locus_tag="PKZ74_05010"
     CDS             1487..2971
                     /gene="crtI"
                     /locus_tag="PKZ74_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM92152.1"
                     /translation="MKKALIVGSGLGGLTAAIRLAKIGYQVEMVEKYHQPGGRLNQLK
                     KDGFTWDMAPTFFSMSYEFKEFVNSTGIKMPFEFVELDPLYAVNFANSDKFYLIHKNL
                     EKLAKEFEAIEPGFEAKMKRYLEGAGKLFHDTEDVIIRKNFNSFAHFLFQMSKVPWGH
                     APKMIRSMWKEMDRHFESHEVKVIFSLVAFFLGATPFDTPAVYSLLSYTEMVHDGYHN
                     VKGGMYKIVEGLMEEFKRNNITIRYNTEITGFEQEGGNLDYLVDSQGNRYYADVFVIN
                     ADAAGFRSQIFNRKNFSEEKLDRMKWTLAPFTMYLGVKGKVSSLHHHNYFLGNNFEEY
                     AGKIFKNSISLSKPYYYVNVNSRHNPECAPEGSESMFILCPVPDLRYKPDWSDREELA
                     NNILTDLSQRIGFDIRSNLASLTIMDPINWQDTFNLYRGSGLGLAHDLNQIGGFRPKN
                     YDEVFGNVFYVGASTIPGTGLPMTVISSRLVTERIQEKYGLVLR"
     gene            2952..3788
                     /locus_tag="PKZ74_05015"
     CDS             2952..3788
                     /locus_tag="PKZ74_05015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPM92153.1"
                     /translation="MDLFLDNALACSEITTKKYSTSFSLGVRLLGKPLRKPIYAIYGF
                     VRFADEIVDTFHNQDKRAEIRRFEAETFEAISKGFSTNPILHSFQWVVSQYGIEDEMI
                     KSFLRSMKMDLCPNSKDYDAQKYHHYIYGSAEVVGLMCLKVFTYKDKNYTVLVHPARK
                     LGEAFQKVNFLRDIKSDLEDRDRFYFPGVDFNNFDNNAKREIESDIQKDFDEAMAGIR
                     NLNREAKLGVYLAYMYYQKLFDKIKRTDASFILKKRYRISNFRKMLLLAGCYLKVKLG
                     SI"
     gene            3775..4485
                     /locus_tag="PKZ74_05020"
     CDS             3775..4485
                     /locus_tag="PKZ74_05020"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HPM92154.1"
                     /translation="MEVSDGMEWQKFTYALLLAGSVLIPFILSFDKKVAFFRNWKYLF
                     PATLITAAVFIIWDIYFTKHNVWWFNKDFVTGLFIQGLPVEEWLFFIIIPYCCIFIYE
                     VSRAYLKKEWKGFIIPVNIILFIIFLVITLVNIDRIYTAVNFGMATFIIGLQFFLKTH
                     KTWMFHFYVAYALSLIPFFLVNGVLTSMPVVGYNDEENLAMRIYTIPVEDFAYLVNLL
                     LMNLTLYEFFKSRSLKRR"
     gene            complement(4490..5965)
                     /locus_tag="PKZ74_05025"
     CDS             complement(4490..5965)
                     /locus_tag="PKZ74_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM92155.1"
                     /translation="MKNIIILLIVVFPGITGFSQGWVSISGTVYDVDNGMPIPGQSVT
                     IMSDSANGTFYYNVVVTDSLGHYFDLLPVAPGTVNSITVQVTDCNGLIMSFAIWINPN
                     TSQYIQDFYICTATVICQAWFTFRPEPEGSATSYRFLDASTGNGYTTFWSFGDGTFSE
                     EQNPLHAFPGPGTYEICLTIQGTSCQDAFCMSLTISDTVYRQIYGQVFAGNLPVQSCE
                     VYLYSQAPAGGGYTLLEEGFPVDSNGVFYFTLVPEGVYLLQAVPAGSDSYLPAYFGDA
                     ISWQQAAPIIIGEPENPYNIHLEEIGVGNALSGPGSILGQVNTVGMERSGVGLINILL
                     LNENYIPLGFSDVNEAGKFSFPSVPFGQYFLKAELSGVLSDMVGVMVDQVRPEAMITL
                     NYTGNNVLGQEELGRLEVMARVYPNPSDGPVSVEIMINDDELFRAELFTASGKIVSTR
                     DIFLSPSRNTFSYDLSEYNPGLYILKLSSDKRNGSLKILRK"
     gene            complement(6097..7443)
                     /locus_tag="PKZ74_05030"
     CDS             complement(6097..7443)
                     /locus_tag="PKZ74_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92156.1"
                     /translation="MKEHPEPFDQHLRDSLKGHRLTPSDEARKAFLQEAAAIVPDRKK
                     WYKWYYLPAVLVLIFSAIAVIYFTRTETPSHFVEKSAVDLISSSTGQNPVDPLVNPHL
                     QEVTASASSSSYSDSPFQTDTETESVTESVTETETIKQNEQAVLIPENLHQEKFAVEA
                     DGPDVVQEPVDHEITGLPMTPVMSGYDTLQTHIDIDSTVYTGNDSTPDRTDVYVEKPG
                     RNEYFLVGLTTTPELMFNTLEGDKFINNFGVEAAFGYGKISVHTGIGVSISKGIVENE
                     IEYNSYLGTYNRLDSISFTFSENSGDFIPELFMSTGEAWDDDASYDSLEIVKRYTYLQ
                     VPLTLGFDFLQKEKFSAGVRIGTTMSVLLDSKQLTGNYEPGRNIVIGTSRLTPDRVKT
                     TWLATAGISGTAMLSRKIYLEIEPQARYYYGSVYEDSGKLKKPWSVGVRLAIWYKF"
     gene            complement(7444..8019)
                     /locus_tag="PKZ74_05035"
     CDS             complement(7444..8019)
                     /locus_tag="PKZ74_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92157.1"
                     /translation="MKLEKALIKDVLDNRSNAVEALYDRFAPAMLSLCFRYTGNIQDA
                     EDVLHEGFIKILKSLGDFKPRQEHSLEAWMKRIMTNTALNFLRDHSREKRILDIDSVR
                     EKDFILEDDDVGTDIYSLSVTQDQIMELIAALPLGYRTVFNLYVFEDHSHKEICEQLG
                     CSENTSKSQLRKARALLRARIIEMANLIKVK"
     gene            complement(8149..9486)
                     /gene="gdhA"
                     /locus_tag="PKZ74_05040"
     CDS             complement(8149..9486)
                     /gene="gdhA"
                     /locus_tag="PKZ74_05040"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM92158.1"
                     /translation="MNLEKIMNDLEKKHPGEAEYLQAVREVLESIEETVNENPQFEAA
                     GIIDRIVEPDRILTFKIPWVDDQGKVHVNLGYRVQYNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGGKGGSDFDSKGKSDGEIMRFCQSFMLELWKIIGPETDV
                     PAGDIGVGAKEIGFLYGMYKKLTHEHTGVLTGKGLNWGGSLIRPEATGFGAVYFAQEM
                     LSTRGMSIEGKTVCISGFGNVAWGAVTKVNHLGGKVVSISGPDGYIYDPDGISGEKIE
                     YMLELRASNQDIVKPYSYEFPNAQFHQGKHPWEVKCDVALPCATQNELNAEDARNLVK
                     NGCICVAEGANMPSTPDAVEIFIQNKLLFGPGKAVNAGGVATSGLEMSQNSMKLGWPR
                     NEVDARLHQIMRDIHASCVKHGTEPDGYINYVKGANVAGFLKVANAMIDQGVI"
     gene            complement(9653..11008)
                     /locus_tag="PKZ74_05045"
     CDS             complement(9653..11008)
                     /locus_tag="PKZ74_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM92159.1"
                     /translation="MALVVVLFLVSSGRFAFGQVEPKRHVVGAIDNFFSISIKYNVSV
                     EDLKKANPGITSPKPGDVLIIPTPNVATTPEKHIYKPNRPAGGDPFRIALMVPFYLEQ
                     LGDTSWRQKLETSSIPDIAPFRFVQFYHGFLMAADSLAKKGLNAEIYVYDVDQQVSKA
                     RHAIADPELKEMDLIVGPLLKGAFEEVAAFANDHHIPIVNPLSTRNDILVNNPYVFKL
                     SPSVESQPKLVADLIRRDFIDHNIIFYIPNRVQNTELIGRYSEAIEESIGKGRKEITV
                     IDYATDSIRGFQSHASTSRPNLVVIFSENEVLPAALLNKLNAMKSDYEMTVIGLPEWD
                     KFTTIELGYMMPLNAHVLMANYVDYRSDAIKGFVNAYRVKYYDEPLTYAFSGFDAGYY
                     FMGALMEFGKDFVNHTDEIRIPVIQNQFHFKRAGNDGFDNLNWNILYYYDFSLFRKYS
                     Y"
     gene            complement(11072..>12206)
                     /gene="guaA"
                     /locus_tag="PKZ74_05050"
     CDS             complement(11072..>12206)
                     /gene="guaA"
                     /locus_tag="PKZ74_05050"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPM92160.1"
                     /translation="ILRLPDEFSVIASTGDVPAAGYRIAGEKTYGIQFHPEVYHSTEG
                     MTLLKNFVVGICGCSQSWTPSLFIESTVHELQEMLGDDKVVLGLSGGVDSSVAAVLLD
                     KAIGKNLHCIFVDNGLLRKNEFESVLHSYKDMGLNVKGVDARQRFYMALQGISDPELK
                     RKAIGRTFIEVFDEEAHHIRDVKWLAQGTIYPDRIESASVKGPSATIKSHHNVGGLPD
                     YMKLKVVEPLHSLFKDEVRRIGTALGMKTDLLNRHPFPGPGLGVRILGEVTPERIRIL
                     QEVDDIFISGLKQYGLYDSVWQALAVLLPVNSVGVMGDERTYENAVVLRAVGSTDGMT
                     ADWSQLPYDFLGKMSNEIINRVKGVNRVVYDISSKPPATIEWE"
BASE COUNT         3232 a         3019 c         2725 g         3230 t
ORIGIN      
        1 ttcctcctcc cgggtggaca ctgccaccga ccatgtacag gcctttaata ccggaaaaat
       61 tcggatggcg ccggaaagcg gcaaacatcg aattagaact gtttccatag agtgatcccc
      121 ggtacgaagc ggttttctct tcaatacccc gcggatccat gacaaactca aactgccggt
      181 aacgtccggc atcaattccg gtcattctca gtatcttctc cccgatcttc gcccgggctg
      241 cggtaatcat cgaatcccag tcctgcccgc aattctccgg agtattgatc ataacaaacc
      301 agttttcaca tcccccgggc gcatcatctt ttaccatctt gctgctaata aacaggtaaa
      361 ccgtaggatc atcatggatc acctttttat tgaacaggca atcgaattcc tctttgtaat
      421 cctttgcgaa aaggatatta tgaagcccga gattctccga ttgtgttttc attccccagt
      481 aaaatatcaa agcggaggag gaacgctcat gacagaacca cttttccgga aaaggctttt
      541 gcttcaacaa atgcctgtaa ataaaccaga tgtcggcatc gctgataacc atgtcgtatt
      601 ctatttcacc ctggccggac actctgatac ctgaaatcct tccgtttttt accaggattt
      661 catccacttt actattataa tggaacttaa cgcccttttt cccggcaagt tcagtcagtg
      721 ctgaagcaat gccatagatc cctttttcag gaaaataagc gccggtattg tgctccaaat
      781 gcgggataac catcagggtt gcgggggtca ggtaaggatt cgatccatta taggtggcaa
      841 agcgatcgaa tatattaacc gtattgctgt ggctgaagta attcctgttt acccggtgaa
      901 gggtgctgaa cggcttcagc cggtatagct gcaggaatgc tttcaaaaaa ggggttttca
      961 ggtaattccc tttcaggtga agggaattgc tgatgaaaac gggatcagtc agttcataaa
     1021 ttttcctgct gtcagacagg tacttcagca cacgggccgg atcttcaccg gctttccgtt
     1081 gcagttcatc cgcaaatctt tccggagtgg aaaaagcatg gatcaccatt ccgtcacgga
     1141 aaaaatatcg gcaggtttca tccagcttac gataaggaag atggtcgtcc atcgattcgc
     1201 cggccagttc aaacagttcg gaaacaagcc cggggagggt aaaaagggat gggcctgtat
     1261 cgaaacggta tcctccgtgc cttaattcgg ccattttacc gcccggataa ctgttctttt
     1321 caaaaacatc aacccggtat cctttcaatg ccattctgac agcaacggct attccgccta
     1381 tcccagcacc gattaccgca acttttccgg gcatttttga acattttaaa caaagatatg
     1441 ttttaaagtc tcttgttttt cttaatttcg ggaaccaaat caaaaaatga aaaaagcgct
     1501 cattgtcggt tcaggtttag gtggtcttac cgccgccatc cgtttagcca agatcggtta
     1561 tcaggttgaa atggttgaaa aataccacca gcccggagga agacttaatc agttgaaaaa
     1621 agacggattc acatgggata tggctcccac tttcttcagc atgagctatg aattcaaaga
     1681 gtttgtaaat tcaacgggca tcaaaatgcc ctttgaattc gttgagcttg atccgctgta
     1741 tgccgtcaac tttgccaaca gcgacaagtt ctatctcatt cataagaatc tcgaaaaact
     1801 ggcgaaagaa tttgaagcca tcgagccggg atttgaggcc aaaatgaaac gatacctgga
     1861 aggcgccgga aagctttttc acgataccga agatgtcatt atccgcaaga acttcaacag
     1921 ttttgcccat ttccttttcc agatgtctaa agtaccctgg ggtcacgccc ccaaaatgat
     1981 ccggtcgatg tggaaagaga tggaccgtca ttttgaatcg cacgaggtca aagtgatctt
     2041 ttcactcgtg gccttcttcc tgggcgcaac gcctttcgac acccccgcag tttacagcct
     2101 gctttcgtat accgaaatgg ttcacgacgg ctaccataat gttaagggcg gaatgtataa
     2161 aattgtggag ggtttgatgg aggagttcaa aaggaacaat attacgatca ggtataatac
     2221 ggagataacc ggatttgagc aggaaggggg taacctggat tacctggtcg attcgcaagg
     2281 aaaccggtat tatgccgatg tctttgtgat caatgccgat gcggcgggtt tccgttcgca
     2341 gatttttaac aggaaaaact tttcggaaga gaagctcgac aggatgaaat ggaccctggc
     2401 gccgtttaca atgtatcttg gcgtgaaagg aaaagtctcc tcccttcatc atcacaacta
     2461 tttcctggga aataattttg aagaatatgc cggaaaaatc tttaagaatt ccatcagcct
     2521 ttccaaacca tattattatg tgaatgtcaa ttcgaggcat aacccggagt gtgcacccga
     2581 agggagcgaa agcatgttca tcctttgccc tgtgcctgac ctccgttata agcccgactg
     2641 gtcggaccgc gaggaactgg ccaataatat cctgactgac ctgtcgcagc ggatcggatt
     2701 cgatatcagg tccaacctgg cctctctgac gatcatggac cccattaact ggcaggacac
     2761 tttcaatctt taccggggaa gcgggctcgg cctggcgcat gacctcaacc agatcggggg
     2821 attccggccc aaaaattatg atgaggtgtt cggaaatgta ttctacgtcg gcgcatccac
     2881 catacctggc acaggcttgc ctatgacagt gatcagttca cgacttgtaa cggaaagaat
     2941 tcaggagaaa tatggacttg ttcttagata atgctctcgc ttgcagtgag ataaccacaa
     3001 agaaatacag caccagtttt tcattgggtg tcaggttgct gggcaaaccg cttcggaagc
     3061 ctatttatgc tatctacggc tttgtccgtt ttgcagatga aatcgtcgat acctttcata
     3121 accaggataa aagagcggag atcaggcgat ttgaagccga gacattcgag gccatcagca
     3181 aagggttcag cacaaacccg atcctccaca gctttcaatg ggtggtcagc caatacggca
     3241 tcgaagatga gatgatcaaa tcatttctcc gcagtatgaa aatggatctg tgccccaaca
     3301 gcaaagacta cgatgcgcag aaataccatc actatattta cggatcggcg gaggtggtcg
     3361 gcctgatgtg cctgaaagta tttacctata aagataaaaa ctacacagta ctggttcacc
     3421 cggcccgcaa actgggagaa gcttttcaaa aagtcaattt tttacgtgat atcaagtctg
     3481 atcttgaaga ccgtgaccgg ttctattttc cgggcgtgga tttcaataat ttcgacaata
     3541 atgccaaaag ggagatcgaa tcagatatcc agaaagactt tgacgaagcc atggccggaa
     3601 tccggaatct gaacagggaa gctaaactgg gcgtatacct ggcctacatg tattaccaga
     3661 aattattcga taaaataaaa cggaccgatg cctcttttat cctgaaaaaa cgataccgta
     3721 tctccaattt ccggaaaatg cttcttctgg caggttgtta cctgaaggtt aaacttggaa
     3781 gtatctgatg gtatggaatg gcaaaaattc acctatgccc tgttgctggc cggttcggta
     3841 ttaatcccgt tcatcctcag tttcgacaaa aaagttgcct tcttccggaa ctggaaatat
     3901 cttttcccgg caacattgat aacggctgct gttttcatca tctgggacat ctatttcaca
     3961 aaacataatg tctggtggtt caacaaggat tttgtcaccg ggcttttcat tcagggctta
     4021 ccggttgaag agtggctatt ctttatcatc attccgtatt gctgcatatt tatatacgag
     4081 gtatcccggg catatctaaa aaaagaatgg aaaggtttca tcatcccggt caatatcatc
     4141 ctgttcatca tcttccttgt gattacactg gtaaatattg acagaattta taccgctgtc
     4201 aactttggaa tggcaacctt tattatcggc ctgcagttct ttctgaaaac acataaaacc
     4261 tggatgtttc atttctatgt ggcctatgcg ctcagcctta tcccattttt ccttgtaaat
     4321 ggtgtactga ccagtatgcc ggtggtgggt tataatgatg aagagaacct ggccatgcgg
     4381 atctatacga tcccggtcga agattttgcc tacctggtca atcttttact gatgaatctc
     4441 accctttacg aattcttcaa gtcaagaagt ttaaaaagaa gatgatctgt cattttctga
     4501 gaatcttcaa actgccattc cgtttatccg aactcagttt taaaatatac agtcccggat
     4561 tatattcgct caaatcatat gaaaatgtat tccgggaagg cgacaagaaa atatctctcg
     4621 tggaaactat ttttccggat gcggtgaata attcagcacg gaaaagttcg tcatcattta
     4681 tcataatctc aacgctaacg ggtccatcag acggattggg gtatacccgg gccattactt
     4741 ccagccggcc tagctcctcc tgtcccaaaa cattattgcc ggtgtaattc agcgtgatca
     4801 ttgcttcagg cctcacctgg tcgaccatta cccctaccat atcgctcaaa acacccgata
     4861 attcagcttt cagaaaatac tgaccaaaag gaacggaggg aaagctgaac ttacctgctt
     4921 cgttcacatc gctgaaacct aagggtatat aattctcatt taataggaga atattgatca
     4981 ggccgacacc cgaacgttcc attccgactg tattgacctg accgaggata gatcccgggc
     5041 cgcttaaagc attgcctacc ccgatttcct caagatgaat attatacgga ttctccggtt
     5101 cgccaatgat gatgggcgca gcctgttgcc atgagatggc atctccgaaa tatgcaggca
     5161 ggtatgaatc ggatccggcc ggaacagcct gcaacaggta aacgccttcc ggaaccagcg
     5221 taaaataaaa gacgccgttt gaatcgaccg gaaagccttc ttccagcagc gtgtaccctc
     5281 caccggcagg ggcttgtgaa taaagataaa cctcacagga ctgcacaggc agattcccgg
     5341 caaaaacctg cccgtatatc tgccggtaaa cggtatcgct gatcgtcagg ctcatacaaa
     5401 aagcatcctg gcatgacgtc ccctgaattg tcaggcagat ctcatatgta cccggacccg
     5461 gaaaagcatg cagcgggttc tgctcctcag aaaacgttcc gtctccaaaa ctccagaatg
     5521 tcgtataacc gttcccggtt gaagcgtcaa gaaaacggta ggaggttgcc gaaccctcgg
     5581 gttcgggtcg aaatgtaaac caggcctggc agatgaccgt cgcggtgcat atataaaagt
     5641 cttgtatgta ttgactggta tttggattta tccatatggc gaaactcatt atcagaccgt
     5701 tacaatccgt aacctggaca gtaattgaat tgacggttcc cggagcgacc ggcagaagat
     5761 cgaagtaatg gcccaatgaa tcggtaacca cgacattgta atagaaagtt ccgtttgcag
     5821 agtccgacat gatggtcaca ctctggcccg gtatcggcat cccgttgtca acgtcgtaca
     5881 ccgttccgct gatggatacc catccctgag aaaaaccagt aataccaggg aaaactacta
     5941 tcagtagaat tattatattt ttcatgacac actatttaaa attccaaatt ccaaattcca
     6001 aattccacct tatgaccact ttatgaccac cttataacaa ccttatgaca accttatcac
     6061 caccttatga ccaccttata acaaccttat gaccatttaa aatttatacc aaattgccag
     6121 cctcacccct actgaccatg gttttttcag cttgcctgaa tcttcataga ccgatccgta
     6181 ataatacctc gcctggggct caatctccag gtatattttg cgtgacagca tcgctgttcc
     6241 gctgatgccg gcagtagcca gccatgtcgt tttaaccctg tcgggggtca gccggctggt
     6301 cccgatcacg atgttccgac cgggttcata gttgcccgtc agttgtttcg aatccagcaa
     6361 taccgacata gtggtaccta tccttacgcc tgccgagaac ttctcctttt gcaggaaatc
     6421 gaatcccaac gtcagtggca cctgcaaata ggtgtaacgt ttcacgattt ccagcgaatc
     6481 atagctggca tcgtcatccc aggcttcccc ggtgctcatg aaaagctccg gaataaaatc
     6541 gccgctgttt tcactaaagg taaaactgat cgaatcgagc cggttgtagg tgccgagata
     6601 gctgttgtat tcgatctcgt tctccacgat tccttttgat attgagacac cgatccctgt
     6661 atgaacagag atctttccat atccgaacgc cgcttcgacg ccgaaattgt tgataaattt
     6721 gtcgccttca agcgtattga acatcagctc aggcgtggtt gtcaatccaa ccaggaaata
     6781 ttcattgcga ccgggttttt caacatacac gtccgttcgg tccggggtag aatcattgcc
     6841 ggtgtaaacg gtcgaatcaa tgtcaatgtg tgtctgcaag gtgtcataac cactcatcac
     6901 aggagtcatt ggcaagccag taatctcatg gtcaacaggt tcctgaacca cgtcgggtcc
     6961 atccgcctca accgcaaatt tttcctggtg caggttttca gggatcagga cagcttgttc
     7021 attttgtttt atcgtttcgg tttcggttac actttcggtt acactttcgg tttcggtatc
     7081 agtctggaaa ggggaatcgg aataagatga tgatgaagca gaagcagtga cttcttgtaa
     7141 atgaggattt accaggggat cgacaggatt ctggccggtt gatgaagata tcagatcgac
     7201 cgcggatttc tcaacaaaat gagatggcgt ctcggtgcga gtaaaataaa tgacagcaat
     7261 ggctgagaag atcagcacga ggacggccgg aaggtagtac catttgtacc acttttttct
     7321 gtccggaaca atggcggcag cttcctgcag gaacgctttc ctggcctcgt cactgggtgt
     7381 gagccggtgc cctttcagcg aatccctgag atgctggtcg aatggttcgg gatgctcttt
     7441 catttatttc actttaatta agtttgccat ttcaatgatc cttgcccgaa gtaaagcacg
     7501 ggcttttcgc agctgggatt tggatgtgtt ttccgagcag cccagctgct cgcagatttc
     7561 cttatgggaa tgatcctcaa aaacatacag gttaaataca gtgcggtacc ccagtggcag
     7621 ggcggcgatc agttccatga tctggtcctg cgtaacggat aaactgtaaa tatccgttcc
     7681 cacgtcatca tcttccagga taaaatcctt ctccctgacg gaatcgatgt caaggattct
     7741 cttctccctt gaatggtcgc gaagaaaatt cagcgccgta ttggtcatga tccgtttcat
     7801 ccaggcctcc agcgaatgtt cctgccgtgg tttaaaatcc ccgaggcttt tcaggatctt
     7861 gatgaagcct tcatgcagta catcctcggc atcctggata tttcccgtat aacggaagca
     7921 aaggctcagc atggcaggcg caaaccggtc gtacagcgcc tctacggcgt tggaacggtt
     7981 atccagcaca tcctttatta gcgccttttc tagcttcatc aacaaatccg ctgagattaa
     8041 aacaccatcg ccctgaatgg ttgcctgtaa atttaaaaaa ataccaatga aaaagaggct
     8101 tctccactct gtgagaaagc ctctcttaaa aagattatgt cagtcagatt aaatgacgcc
     8161 ctggtcgatc atggcattgg ctactttaag gaagccggcc acgttggcgc ctttaacata
     8221 gttgatatat ccgtcgggtt cggtaccgtg ttttacgcag gaagcatgga tgtcgcgcat
     8281 gatctggtgc aggcgggcat cgacttcatt tctcggccag ccgagtttca tggagttctg
     8341 cgacatttca aggcctgagg tggctacacc accggcattg actgcttttc cgggtccgaa
     8401 caggagtttg ttctggataa agatttcaac tgcatcggga gtggagggca tattggcgcc
     8461 ttcagcgacg caaatacatc cgttcttcac caggttgcgg gcatcttctg cattcagctc
     8521 attctgtgta gcgcagggca atgccacatc gcacttcact tcccacgggt gcttgccctg
     8581 gtggaactgg gcattcggaa actcatacga ataaggtttt acgatatcct ggttcgatgc
     8641 acgcagttca agcatatatt cgatcttttc acctgaaatg ccatccggat catagatata
     8701 accatcaggt ccggagatgg acacgacttt tccgccaagg tggttcactt tagtgactgc
     8761 accccaggcc acgtttccga aaccggagat acaaaccgtt ttaccttcta tcgacattcc
     8821 acgggttgaa agcatttcct gtgcgaaata cacggctccg aatccggtgg cttccggacg
     8881 gataaggctt ccgccccagt tcaatccttt accggtcaga acacccgtat gctcatgggt
     8941 tagtttcttg tacatcccat agaggaatcc gatctctttg gctccaacac caatatcacc
     9001 tgccggcaca tcggtctcgg gtccgataat cttccaaagt tccagcatga acgactggca
     9061 gaaacgcatg atttcaccgt ctgatttgcc tttcgagtca aaatcagaac cccctttgcc
     9121 accacccatc gggagcgtgg tcagggcatt cttgaatatc tgctcaaatc ccaggaactt
     9181 caaaatgctc aggttcacac tcgggtggaa acgcagcccg cccttataag gcccgatagc
     9241 attgttgtac tgaacacggt aaccgagatt gacatgcacc ttgccctgat cgtcaaccca
     9301 cggaatcttg aatgttaaaa tccggtcagg ttccacaatc cggtcgatga tcccggcggc
     9361 ttcaaactgg ggattctcat ttaccgtttc ttcgatcgat tccaaaacct cgcgtacagc
     9421 ctgcaggtat tcagcttcac ccgggtgttt cttctcgagg tcgttcatta tcttctctaa
     9481 attcatgatg taaagattta tagtttaatt atgttaaaat ttaacactgt taaaaaatta
     9541 acaggacaaa attagtgctt tatcttatac aaaaaagaaa aatggctgaa gaatcaaata
     9601 attttttgaa aagagcagaa tagttgaaaa accagtccga attcggtcta aatcaatagg
     9661 aatatttccg gaaaagtgaa aaatcataat aatacagaat attccagtta aggttatcga
     9721 aaccatcatt gcctgcccgc ttgaaatgaa actggttctg gataacgggg atacggattt
     9781 catccgtatg gttaacaaaa tctttcccga attccataag tgcccccata aaataatagc
     9841 cggcatcgaa tcctgaaaaa gcataggtca gcggctcatc gtagtatttc accctgtagg
     9901 catttacaaa tccttttatg gcatcggaac ggtaatcgac ataattggcc atgagaacat
     9961 gcgcattgag cggcatcata taccccagct caatcgtggt aaatttatcc cattcgggca
    10021 acccgatcac cgtcatctca taatcggatt tcatggcatt cagcttattc agcaatgctg
    10081 ccggcaggac ctcattctca gaaaaaatga cgaccaggtt agggcgtgag gttgaagcat
    10141 ggctctgaaa accgcggatg ctgtcggtag cgtaatcgat caccgttatt tcctttcttc
    10201 ctttacctat cgactcttcg atcgcttccg aatatcttcc gatcagctct gtgttctgga
    10261 cacggtttgg gatataaaag atgatgttat ggtcgatgaa atcgcgccgt atcagatctg
    10321 ctaccagttt cggctgtgat tctactgatg gcgacaattt gaagacatag ggattattca
    10381 ccaggatatc atttctcgta gagaggggat tgacaatagg aatatgatgg tcattggcga
    10441 atgcagcaac ctcttcaaat gctcccttaa gaagagggcc gacaatgagg tccatctcct
    10501 tcagctcagg gtcggctatg gcatgccggg ctttggatac ctgctgatcg acatcataga
    10561 cataaatttc ggcattcaat cccttttttg ccagggagtc ggcagccatc aggaaaccat
    10621 ggtaaaactg aacaaaacgg aatggcgcaa tatcagggat gctggaggtt tcaagctttt
    10681 gcctccaact cgtatcgccg agctgttcca ggtaaaaagg caccatcagc gctatccgga
    10741 aaggatctcc ccctgccggc ctgttcggtt tgtaaatatg cttttcgggc gtcgttgcca
    10801 cattaggtgt cgggatgatc agcacatcgc ccggtttcgg gctggtaatc cccggattgg
    10861 cctttttcag gtcttcaaca gacacattgt atttgatcga aatactgaaa aaattatcga
    10921 ttgctccgac cacatgcctc ttcggttcaa cttgcccgaa agcaaaccgg ccggatgata
    10981 ccagaaataa aacaacaaca agcgccaaaa ggcccctttt aattaatttc ctgatatcca
    11041 taactttccg tattttggtt aatgatcaag cttattccca ttcaatggtg gcaggaggtt
    11101 tggaactgat gtcataaacc actctgttca cccctttcac cctgttgatg atctcattcg
    11161 acattttacc caggaaatcg tacggaagct ggctccagtc ggctgtcatg ccatcggtcg
    11221 acccgacggc cctcagcacc acggcatttt cataggtcct ttcatctccc atcaccccca
    11281 ccgaatttac cggtagcaga acagcaagag cctgccagac actgtcgtac aaaccgtact
    11341 gtttcagacc gctgataaag atatcgtcga cctcctgcaa aatcctgatc cgttccgggg
    11401 tcacttcacc cagaatcctg acgcccaggc ccggtccggg aaacgggtga cggttcagca
    11461 ggtcggtctt cattcccagc gcggtgccga ttcgcctgac ctcgtctttg aacagggagt
    11521 gcagcggttc caccaccttc agcttcatat aatcgggcaa accgccgaca ttatggtgcg
    11581 acttgatcgt tgccgatggc cctttgacag atgccgactc gatgcggtcg ggatagatcg
    11641 tgccctgggc cagccatttc acatcgcgga tatgatgcgc ctcttcatca aaaacctcga
    11701 tgaaggtcct gccgatggct tttcttttaa gttccgggtc ggagatgccc tgcagggcca
    11761 tatagaaccg ctgcctcgca tccacccctt tgacattcag ccccatatcc ttgtacgaat
    11821 gcaggaccga ttcgaactca tttttcctca gcaggccgtt atcgacaaat atgcaatgca
    11881 ggtttttgcc aatagcttta tcgagcaaca cggctgccac agaggagtcg acccctcctg
    11941 acagcccgag gacaacttta tcatcaccaa gcatttcctg caattcatgc acggttgatt
    12001 cgatgaacag gctgggagtc caggattgag aacatccgca tatcccaacg acaaagtttt
    12061 tcagaagcgt catcccttcg gtggaatggt aaacttcagg atggaactgg attccatagg
    12121 ttttctcccc tgcaatgcgg tagccggcag caggtacatc gccggtgctg gcaatcacac
    12181 tgaactcatc gggcagcctc aggata
//
LOCUS       DAOPNH010000066        12164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5457_length_12164_cov_6.136345, whole genome shotgun sequence.
ACCESSION   DAOPNH010000066 DAOPNH010000000
VERSION     DAOPNH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_12164_cov_6.136345"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKZ74_05055"
     CDS             complement(<1..917)
                     /locus_tag="PKZ74_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM92161.1"
                     /translation="MKTIRIFLYIAVASFLFACSEDKMDEINKERNDALEMAAKNILP
                     DIMLKTAFEVAGTDIPWYATVYIEHSAGTWAQSATADKRVAQNDASLFNNNWMSIYDV
                     LNACKAVIDKTDPVNGDESTNNWARGIAYVMTAYNLATATDMWGEVPWTEALQGAEEL
                     TPKYDKQSFLYTEIQRLLDSAIVNLGKTSIKYGAFDYIYGGDETKWIKAAWSLKARYY
                     LRLSQRDANAYDKALACIANGFTSNDDNFLFAAYESSASGENPWYQFLADRTHLSASK
                     TLYDLMNERVDPRIAAYFTQIDAFVHQVI"
     gene            complement(937..4200)
                     /locus_tag="PKZ74_05060"
     CDS             complement(937..4200)
                     /locus_tag="PKZ74_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM92162.1"
                     /translation="MRKFTLFAVFLLFVGMQAALAQMVVRGTITDAKDGTPIPGVSVV
                     VKGTLTGTVSDAMGKYELDVPAGYNELIFSFIGMRTKEMKIDNKSVVDIAMDEDVVGL
                     DEVVVTALGISREKKSLGYSTQEATGVEISKTANPNFQTALSGKFAGVEVRQSSGMPG
                     APSQIFIRGARAFSGDNTPMYVVDGMPIVSTPDYEQASGGVTGSYYSNRALDIDPNNI
                     ESINVLKGQAAAALYGMRASNGVIIITTKKGKVGQVGKPVSVSLSTNFALDDPARLPE
                     RQTSYAQGYYNEFYPAFSYSWGPKLADLANDPTYGGNNYEGHAGEFFDPYKGQWVIPQ
                     NYNNVENFYDKGGQTFNNNIAINGVSKDASYALGFSTTNQKGIIDGTGMDRYNANASA
                     TYNLGPKWKAGFSGNFSTVKLDKVPSGNDSWLFTVYGSPPSFDLMGTPYYQEGSLGQY
                     RQISYRRGTVGENPRWALENNYFLEKTNRFFGNMFLEYKPFEWMNVRYQLGVDAYGTD
                     NEDLYQMGSTATGQVLPTGANYPTPDNPVFGYREPTGGRIHNYGINRNTMNSLLNITF
                     KKRWDNFGGMLVIGNEYYNNRSRYWDMLGTGFTTPGWNNMANTSTQTASESKYWDRTV
                     GFYGNLMVDWKSLLFLNLTGRMDNVSSMPRGNRQFFYPSASLGFVFTELEGLKDNKIL
                     SFGKVRVSWAQVGQPGDFRQNVYVLGGAGSGFLDDGILYPLGGISGYKPSNTIYDPDL
                     TAQNTNTLEGGIELKFFQNRLGIDYSYADQTAEGQIFGVPLAGSTGYATYYTNAGKMT
                     SKAHEIILNGTPVKTDNFTWDLLVNFTKVKNVCEELAEGVESIFLGGYETPNIRASAG
                     DTYPAIYGNLFARDEQGRILVDEDPNSYYYGMPYQGEFGKIGEVTPDFIMGLTSNMYI
                     YKMFTLTFRADWKQGGDMYSGSNRLMNLYGTSAVTEDRETPWVFNTDNGFRWDGYKAD
                     GTPTDIQRGGVEDFYAYPDLYNDVFGNIEEAAVYETSFIKIREIVLSFDFPEKVIKSL
                     RLQNLSLNLFTRNILLWTTLPNFDPEASQGMGNMQGGMDYMSLPQTTTYGAGLNITF"
     gene            complement(4468..4905)
                     /locus_tag="PKZ74_05065"
     CDS             complement(4468..4905)
                     /locus_tag="PKZ74_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92163.1"
                     /translation="MTDNGSENIVTSFQKEIRKGSFICLVVQCMALTYFLIIALLFLA
                     GLLFSGTVNEAVGTYYGLEEEFASKYFLFIITGTVLFLTIVAGIIIFMMKYRAGFYIF
                     IAAILVTIILDLTFLKFDWVRYLIQSGFIFLLGIAHFSRKCYR"
     gene            complement(4943..6466)
                     /locus_tag="PKZ74_05070"
     CDS             complement(4943..6466)
                     /locus_tag="PKZ74_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPM92164.1"
                     /translation="MTIVITGSNLKIKDVVNVARHNEKVELHPDAVKRIETCRAMLEK
                     KIVAREIMYGVNTGIGEFSEIVLTDEQVQQFQRYLIYNHAAGIGDPMPIEWVRGAMVG
                     RINVHAHGNSGNRPVITQTLVEMLNKGVTPYVCQKGSVGACGDLAPMSQIALLMMGEG
                     KAYYQGELLDGKEALDRAGIPVPGLQARVGLATINGSNVLTAMSALFLYDANNFLKQA
                     EIAASMSLEALKANMKPYNIKLHSLRGFPGAVRSAAAINKLVSGGDLKEGKIKCKVQD
                     AYSMRSTPQVIGAAHDAIAWAVKQVETELNGVGDNPIFIPEDNLTLTGANFQGSPVAL
                     PLDMAGAAITMVCVLSERRMNRLNNPALSVGLPAFLTKGAGMFSGLMLSQYTADMQIV
                     EQRILSAPASIQSIPAAADQEDFVSMGMNVALKNFQIIDNAYGILGIELMAAAQALDF
                     REFSFGNGVQKAKEVIRRHVAFLDVDRPLYDDHNTMKALVKSCEILTEVEKEIGSLE"
     assembly_gap    6823..6922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6923..7935)
                     /locus_tag="PKZ74_05075"
     CDS             complement(<6923..7935)
                     /locus_tag="PKZ74_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPM92165.1"
                     /translation="MGEKFTIPKGTRDFTPVEMIRRNFIFDTVKKVFSHYGYVPIETP
                     AMENLSTLMGKYGEEGDKLLFKILNSGNYLAGIGPEEINPENPGPLTQKIAEKGLKYD
                     LTVPFARFVVQHQNEITFPFKRYQIQTVWRADKPQKGRYREFFQCDVDVIGSHSLMNE
                     VELVQIIADVFGRLDIRVVIRLNNRKILAGIAEIAGARDRFTDLTIALDKLDKIGLEK
                     ALGELKEKGFTEAEVEKIMPFTAITGTFEERLRWLEGHLSGSETGLKGIAEMRSVFTR
                     IKFLRLEEICELDITLARGLNYYTGTIFEVKATEVEIGSICGGGRYDDLTGIFGLPGV
                     SGVG"
     gene            8031..9227
                     /locus_tag="PKZ74_05080"
     CDS             8031..9227
                     /locus_tag="PKZ74_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM92166.1"
                     /translation="MKILQVANKFPYPAKDGGAIATLSLSRSFAAQGHAVTVLAMNTS
                     KHFTDISRVPEDLNAKIRLIGVPVNTDIRILPLVRNFLFSRLPYNAERFVDTRFRNAL
                     EDLLKSESFDIIQLEGLYLAPYLEAIRKFSSARVVMRAHNIEHEIWERSIAGHAGIRK
                     LYIASLARRIRRMELEFLNKYDAVVPITERDHLVLQKLGCRLPAHVVPMGVNPDELVP
                     DFSRMEFPSVFHIGALDWMPNQEGLTWFLEKVWEKVLKHHPDLKFYLAGRNAPDHFKS
                     LTYPNVSYLGEVADAYDFIRSKTVMIVPILSGSGMRIKIIEGMALGKAIVTTTIGTEG
                     IATTSGENLFVADDPDEFAQIVCNLVENREKCVAIGENARKFVTEYYDNDVITRSLIL
                     FYENLK"
     gene            9224..>10245
                     /locus_tag="PKZ74_05085"
     CDS             9224..>10245
                     /locus_tag="PKZ74_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM92167.1"
                     /translation="MTIALLIFWISLLLVVHTYVFYPFILQVLSFRKKGNRMVYEAGG
                     ELPVVSVIIAAHNEESCIAEKIESIYSGTYPYDRLEVLVGSDNSDDRTVEILAALQKR
                     FPTLQFFDFRERRGKGSVVNDLVGASKGSLLVLTDANVMFDRETLFYLVRHFRNPAIG
                     LVDTNMVNMGMKKDGISYQERAYISREVRIKDMEGRLWGAMMGPFGGCYAIRREDYCQ
                     VPANFLVDDFYINMTIFEKGKLAVNDLNSIVFENIPDELKVEFRRKVRIGTGNFQNLR
                     RFWRLAFTMRYGFAFISHKIIRWFGPFLLLAALVANIYLAVFSRVYLFILAGHLLVYL
                     LPFFDR"
     assembly_gap    10246..10345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10346..11565)
                     /locus_tag="PKZ74_05090"
     CDS             complement(<10346..11565)
                     /locus_tag="PKZ74_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPM92168.1"
                     /translation="MKKINALVLKTYSGPLLVTFFIALFILLMQFMWMYIDDLVGKGL
                     EWYVIAELLFYASFTFVPLALPLAILLSSLMTFGNLGEHYELVAMKSAGISLRSIMKP
                     LIILSLCISAFAFYFSNNVLPVANLKFKSLLYDIRKKKLAVNIREGIFYNDMEDYVIR
                     VGKKGKDGNRIYKVMIYNHTENRGNIDVTVADSGIMESTPDGRYLIFTLYDGFNYQDI
                     VDQKDSKNQQPFQRTSFKIQQRKFDLSAFELNRTNEELFKNNYQMLNIGQLNYFIDSL
                     SRSLVERKENLARNFPRNYQLYLNNDTAYVPSTDSVTVVPGDFLSGFSTDDQIRIVSN
                     ALTSARGIKKNLDFQSDAFRVSNESLVKHKIVWHRKFTLSFACLVMFFIGAPLGAIIR
                     KGGLGLPTVISILF"
     gene            11705..>12164
                     /locus_tag="PKZ74_05095"
     CDS             11705..>12164
                     /locus_tag="PKZ74_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92169.1"
                     /translation="MKKLLLIAMALIIGVVAYSQNKVQLSKEMREQTVTLTRQNDIAE
                     EIVFGNFSDPTVSNSKAAPIETLIGTTKYDLMTNSMIQNRLYLHADNTIGAVWTMGLG
                     TEPAFSDRGTGYNYYDGAQWGDAPTVRIEPERTGWPAYAPWGPNGEIIAAH"
BASE COUNT         2966 a         2892 c         2940 g         3166 t
ORIGIN      
        1 tgtatgacct gatgaacgaa cgcgtcgatc tgcgtgaagt atgcagcaat tctcgggtcg
       61 acgcgttcgt tcatcaggtc atacagcgtt ttgctggcag atagatgggt acggtcggcc
      121 aggaactgat accacggatt ttcacccgat gcggatgatt cgtatgcggc aaagaggaaa
      181 ttgtcatcat tgctggtaaa tccgttggca atacatgcca gcgctttgtc gtaagcatta
      241 gcgtcccttt ggctgagtct caggtaatac cgggctttca gcgaccaggc agccttgatc
      301 cacttggttt catcgccacc atagatatag tcgaatgcac catatttaat ggaggttttg
      361 cccaggttaa caatggcaga gtcgagtaac cgctgaattt cggtgtagag gaatgattgt
      421 ttgtcgtact tgggcgtgag ttcttctgct ccctgcaaag cttccgtcca gggtacttca
      481 ccccacatat cggttgcggt tgcaaggttg tatgccgtca tcacataagc gatacctctg
      541 gcccagttgt tcgtcgactc atcgccgtta accggatcgg ttttatcgat aacggcttta
      601 caggcgttga ggacatcgta gatgctcatc cagttattgt tgaacaggga ggcgtcgttc
      661 tgggccaccc ttttgtcagc ggttgccgac tgtgcccagg ttccggcgct atgttcgatg
      721 tatacagtgg cataccaggg aatatcggta ccggccactt caaaggcggt tttcagcatg
      781 atgtccggga ggatgttctt agcggccatc tccagggcat cgttgcgttc cttgttgatt
      841 tcatccatct tatcttcact gcaggcaaag aggaaggatg caacggcgat gtaaaggaat
      901 attcttattg ttttcataat cattactatt tacaggttag aaagttatgt taagaccggc
      961 gccgtatgtc gtagtttggg gcagggacat atagtccatc ccgccctgca tgttgcccat
     1021 accttgtgaa gcttcgggat cgaaattcgg cagggtggtc caaaggagga tgttcctggt
     1081 gaacaggttc agcgacaggt tctgcaaacg caatgatttg atcacctttt cggggaagtc
     1141 gaatgagaga acgatctccc tgatcttgat gaaggaggtc tcgtacacag cggcttcttc
     1201 tatatttccg aagacgtcgt tgtacaggtc aggataagca tagaaatctt ccacgccacc
     1261 cctctggatg tcggtcgggg ttccgtcagc tttgtaaccg tcccagcgga atccgttgtc
     1321 ggtattgaat acccacgggg tttcgcggtc ttcggtaacg gccgaggtgc catacaggtt
     1381 catcaatcgg ttggagccgg agtacatgtc gccaccctgc ttccagtctg cgcggaaagt
     1441 gagggtgaac atcttataga tatacatgtt gctggtgagg cccatgataa agtcgggggt
     1501 cacttcgccg atctttccga attcgccctg gtacggcatg ccgtaatagt agctgttcgg
     1561 gtcttcgtcg accaggatac ggccctgttc gtcacgtgca aacaaattgc catagattgc
     1621 gggataggta tcaccggcag aagcacggat attcggggtt tcataaccgc caaggaaaat
     1681 gctctccacg ccttcagcca gttcctcgca cacattcttc accttggtaa agttcaccaa
     1741 cagatcccag gtaaagttgt ctgttttaac cggtgttccg ttcagaataa tttcgtgtgc
     1801 cttggatgtc attttaccag cattggtata gtatgtagca taaccggttg atccggccag
     1861 cggtacgccg aaaatctgtc cttcagctgt ctggtcggca tatgaatagt caattcccag
     1921 tctgttctgg aagaacttca gttcgatacc gccttccaga gtattggtgt tctgagccgt
     1981 cagatccgga tcgtagatgg tgttgctggg tttgtaacca gaaataccac cgagcgggta
     2041 aaggatgccg tcgtccagga atccgctgcc agccccgccg aggacataga cattctgcct
     2101 gaaatcgccg ggttgtccga cctgcgccca ggagacgcgg actttaccga agctgaggat
     2161 cttattgtct ttcaggcctt ccagttcggt aaacacgaat ccgagtgatg cggaaggata
     2221 gaaaaactgg cggttgcccc tgggcatcga tgacacattg tccatacgac cggtcaaatt
     2281 gaggaacaac aagctcttcc agtcaaccat gaggttgccg tagaaaccga ccgtcctgtc
     2341 ccaatacttc gattcggaag cggtctgtgt tgatgtgttg gccatattgt tccatcccgg
     2401 cgtagtaaat ccggttccca gcatatccca gtacctcgac cggttgttat agtattcatt
     2461 cccgataacc agcattccgc cgaagttgtc ccacctcttt ttaaaggtga tgttgagcag
     2521 ggagttcatc gtgttgcggt tgatgccata gttatgaata cgtccgccgg ttggttccct
     2581 gtaaccgaag acagggttgt caggggtagg ataattggcg ccggtcggca ggacttgtcc
     2641 ggtagcggtg ctacccatct ggtataagtc ttcgttatcg gtgccataag catcgacacc
     2701 gagctggtac ctgacgttca tccattcgaa cggtttgtat tccaggaaca tatttccgaa
     2761 gaaacggttg gttttttcga ggaaatagtt attttccaga gcccatctcg gattctctcc
     2821 gacggtaccc ctgcggtagc tgatctggcg gtattgaccg agtgatcctt cctggtaata
     2881 gggagttccc atcaggtcaa aggaaggcgg tgaaccgtaa acggtgaaca gccagctgtc
     2941 gttgccggaa ggaactttgt cgagcttgac cgtggagaag ttaccggaga aaccggcctt
     3001 ccatttcggg cccaggttat aggtggctga tgcattggcg ttataacggt ccatcccggt
     3061 tccgtcgatg atccctttct gattcgttgt actgaatccc agggcatagc tggcatcctt
     3121 tgaaacaccg tttatagcga tgttgttgtt aaaggtctga ccacccttat cgtagaaatt
     3181 ctccacattg ttataattct gtgggatcac ccattgtcct ttataaggat cgaagaattc
     3241 accggcatgg ccttcataat tatttccgcc gtaggtcggg tcgttggcaa ggtctgcaag
     3301 tttcggcccc cacgaatagg aaaatgcagg atagaactcg ttgtagtaac cttgtgcata
     3361 cgacgtttgc ctttcgggca gacgggccgg gtcatcgagg gcaaaattgg ttgacagaga
     3421 caccgagacc ggtttgccta cctggccgac tttgcctttt ttggttgtaa tgatgataac
     3481 gccgttggat gcacgcatcc cgtaaagggc agcggcagcc tggcctttca gaacgttgat
     3541 cgactcgatg ttgttcgggt cgatatccaa tgccctgttg ctgtagtatg agccggtgac
     3601 gccaccgctc gcctgctcgt agtcgggcgt actgacgatt ggcattccat cgaccacata
     3661 cataggagtg ttgtcaccgc taaatgcacg ggcaccacgg ataaagatct gtgacggtgc
     3721 tcccggcata ccggatgact ggcggacttc gacgccggca aatttcccgg aaagtgccgt
     3781 ctggaagttc gggttggcag tctttgatat ctccaccccg gtcgcttcct gtgtcgaata
     3841 acccagtgat ttcttttccc tggagatacc caaagctgtg acgaccactt catcaagacc
     3901 gacaacgtct tcatccatgg caatgtcgac aaccgatttg ttgtcaatct tcatctcttt
     3961 ggtgcgcatc ccgataaacg aaaagatgag ctcattgtag cctgcaggaa catccaattc
     4021 atattttccc atggcatcgg agactgttcc cgtcagcgtg cctttaacga caaccgaaac
     4081 ccccgggatt ggagtcccgt cttttgcgtc agttatcgtc cctctgacca ccatttgggc
     4141 caaagcggct tgcatcccta cgaaaagtag aaatacggca aaaagtgtaa attttctcat
     4201 aaaagtaatt tttggttaat agaaaattga actcgcttct tctactgcaa tattacaata
     4261 ataaatatag aaatacaagc gcaccagatg aaaaaatttg accaaaaatt aacactaatt
     4321 ttattttacg tattgaaatt gtgatatgta gcctttttgc gaagaaaaaa actgactatt
     4381 aatttatacg gagtcttact ttatcgtcga aaatccggat cagcccctta cagaacctat
     4441 tatttcgaac agattgaatt agaatgacta gcgataacat ttcctggaaa aatgggcaat
     4501 acccagcagg aaaataaatc cggattgaat gaggtaccgg acccagtcaa atttcagaaa
     4561 agtgagatca aggattattg tgaccaggat agcggcaata aaaatataaa atccggctct
     4621 gtacttcatc ataaaaatga ttattccggc tacaatcgtc agaaataata cagtaccggt
     4681 gataatgaac aaaaaatact tcgacgcaaa ttcttcctcc aggccgtaat aagtgcctac
     4741 cgcttcattg acagtacctg agaagagaag ccctgcaaga aacagcagtg caatgatcag
     4801 gaaataggta agggccatgc actgtaccac caggcatatg aatgaccctt tcctgatctc
     4861 cttttgaaaa gatgtgacaa tattttctga tccattatcg gtcatgatga tctgaatgaa
     4921 aatgaaccag ctaagttaat gattattcca ggctgccgat ctctttttcg acttctgtca
     4981 ggatttcaca cgattttacc agggccttca tggtgttgtg gtcgtcgtac aatggccggt
     5041 cgacatccaa aaatgccaca tgcctgcgga tgacctcctt ggctttctga accccgtttc
     5101 caaagctgaa ttcgcgaaaa tcgagcgcct gcgcggctgc catcagctca atgcccagga
     5161 ttccgtatgc attgtcgatg atctggaaat tcttgagcgc aacgttcatt cccatcgaaa
     5221 caaagtcttc ctggtcggct gcggcgggaa tggattggat ggatgccgga gccgagagga
     5281 tcctctgctc gacgatctgc atatcggcag tgtactggct cagcatcagc ccggagaaca
     5341 tgccggcgcc tttggtgaga aatgcaggca gccctacgct cagggccggg ttattcagcc
     5401 tgttcatcct tctttcggat aaaacgcaca ccattgtaat agccgctccg gccatatcaa
     5461 gcggtaaggc caccgggctc ccctggaaat tggcccccgt gagtgtcagg ttgtcttccg
     5521 ggatgaagat cgggttgtcg ccaacgccgt tgagttcggt ttccacctgt ttaaccgccc
     5581 aggcgatggc atcgtgcgct gcgccgatca cctgcggtgt cgatcgcata gagtaggcat
     5641 cctggacttt gcatttgatc ttcccctctt tcaggtcgcc gccggaaacc agcttgttga
     5701 tagccgccgc gcttcttaca gccccgggaa atcccctcag agagtgcagc ttgatattgt
     5761 atggcttcat attggctttc aacgcttcga gcgacatgga agcggcgatc tcagcctgtt
     5821 tcagaaagtt attagcgtcg tacaggaaaa gggcgctcat ggctgtcagt acattcgatc
     5881 cgttgatcgt agccaggccg acccgtgcct gcaatcccgg aaccggtata ccggcacggt
     5941 caagggcttc cttaccatct agcagttcgc cctggtaata ggctttgcct tcgcccatca
     6001 taagcagggc gatctgcgac atgggagcca ggtcgccgca ggcgccaacc gaccctttct
     6061 ggcatacata gggcgttacc cctttgttca gcatctccac cagcgtctgg gtgatcaccg
     6121 gccggttgcc cgaattgccg tgggcatgta cgttgatccg gcctaccatg gcgccgcgca
     6181 cccattcgat gggcatcgga tcgccgatac cggctgcatg attataaatg agataccgct
     6241 gaaactgctg aacctgctca tccgtcagca caatctccga aaattcaccg atgcccgtat
     6301 tgacaccata catgatctcg cgtgcaacga tcttcttctc cagcatcgcc cggcatgttt
     6361 cgatcctttt tacagcatcc gggtgtaatt ctactttttc gttatgacgg gccacattga
     6421 ccacgtcttt tatcttcaaa ttggaaccag ttataacaat agtcataatt ttaaatttta
     6481 aattttgaat tatttataat gaatttgcaa tgaggaagaa tttagaattg aaaaacctga
     6541 aagttttccg aattaaaaat tcaaaatttc taatttaaaa tttagcaaag tgcggtgcgg
     6601 ttgatcttta ttataaattt aagaatccag ctaaacattt gatagatcat ttatggcaaa
     6661 agtaaaaaat taatctccct gatattatta aagatatatt tttgccgcga aaattcgccc
     6721 tatgataatc cggatcgata aagcagaaaa gctgaaggag atcgtcggga cagccgaaaa
     6781 tctgctcgtc tatttcctga atgatgactg tcccccctgc ctnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnccgactcc ggaaactccc ggcaggccga aaatcccggt
     6961 caggtcatcg taccttccgc ctccgcaaat gctgccgatc tctacctccg ttgccttgac
     7021 ttcgaatata gttcccgtgt aataattcag cccccgcgcc agggtgatgt cgagttcaca
     7081 gatttcttca aggcggagaa atttgatccg ggtaaagacc gatctcattt cggcaattcc
     7141 cttaaggcct gtttccgagc cggagagatg cccctcgagc catctcagtc tttcttcaaa
     7201 tgtaccggtg attgccgtaa aaggcattat tttctccacc tcggcttccg taaatccctt
     7261 ctctttcaat tcgccgagcg ctttttccag gccgatctta tccagtttat ccagggcaat
     7321 agtgagatcg gtgaaccgat cacgggcacc ggcaatttct gcaatgccgg caaggatttt
     7381 ccggttgttc agcctgataa ccacgcggat atccagcctg ccgaaaacat cggcgatgat
     7441 ctggaccagc tccacttcat tcatcagtga atgactgccg ataacatcca catcacactg
     7501 gaaaaactcc ctgtaacggc ccttttgcgg tttgtcggcc cgccagaccg tctggatctg
     7561 atacctttta aatggaaatg tgatttcatt ctggtgctga accacaaacc tggcaaacgg
     7621 cacagtcagg tcatatttca atcctttttc ggcaatcttc tgcgtcagtg ggcccgggtt
     7681 ctccgggttg atctcttccg gcccgatccc ggccaggtaa ttccccgaat tcaaaatctt
     7741 gaacaatagt ttatcgccct cttccccata tttacccatt agcgtggaaa gattttccat
     7801 ggccggcgtt tcgatcggaa cgtacccgta atggctgaat acctttttaa cagtatcgaa
     7861 gatgaaattc cgccggatca tctccaccgg ggtgaaatcg cgggttcctt tcgggatggt
     7921 aaacttttcg cccatagtca gactcgttac agttttaaac ttcgtccaat tttaggcaaa
     7981 ggtcgcattt ttcgtttatt tttgccccat tgcgattata aattcagcgg atgaaaatcc
     8041 tgcaggtagc caataagttt ccttatccgg ccaaggatgg cggagcaatc gccacgttga
     8101 gccttagccg gtcgtttgcc gcccagggcc atgctgtcac ggtgctggct atgaatactt
     8161 caaagcattt taccgatatc agccgggttc cggaagattt gaacgcaaaa atcaggctga
     8221 tcggtgttcc ggtcaacacc gatatccgta ttttgcccct ggtaaggaat ttcttattct
     8281 cccggctccc ctacaacgcc gaacggtttg tcgatacacg cttcaggaat gcgctggaag
     8341 acttgctgaa aagtgaaagt ttcgatatca ttcagcttga aggattgtac cttgctcctt
     8401 accttgaagc gattcggaaa ttttcctcgg ccagggtagt catgcgcgcc cacaacattg
     8461 agcatgaaat ctgggagcgg agcattgcgg ggcatgccgg catcaggaaa ttatatatcg
     8521 ccagcctggc ccgtcgtatc aggcgaatgg aactggaatt tctcaataaa tacgatgccg
     8581 tggtaccgat aacggaacgg gatcacctcg tgctccaaaa gctggggtgc cgtttgcccg
     8641 cacatgtcgt gcccatggga gttaatccgg atgaactggt ccccgatttt tcccgaatgg
     8701 aattcccatc ggtttttcat atcggcgcac tcgactggat gccaaaccag gagggcctga
     8761 cctggttcct tgagaaagta tgggaaaagg tgctaaagca tcatcccgat ctgaaatttt
     8821 accttgccgg acggaatgcc ccggatcact ttaaatcgct gacatatccg aatgtcagtt
     8881 acctgggtga agtcgccgat gcgtacgatt ttatccggtc gaaaacggtc atgatcgtcc
     8941 ccatcctttc aggcagcggc atgcgtatca agatcatcga gggcatggcg ctggggaaag
     9001 ccattgtgac aactaccatc ggcacggagg gcattgccac tacttcgggc gaaaatttgt
     9061 ttgttgccga cgacccggat gaatttgcgc aaatcgtttg caacctggtg gaaaatcggg
     9121 aaaagtgtgt agcgatcggt gaaaatgccc gtaaatttgt aaccgaatat tatgataacg
     9181 atgtcataac gcgttcactg attttatttt acgagaattt aaaatgacga tcgccctgct
     9241 catattctgg atatcactgc tgctggtcgt acacacatat gtgttttatc ccttcatcct
     9301 ccaggtgtta tctttccgga aaaaaggaaa ccgcatggta tatgaagccg gaggagaact
     9361 gccggttgtt tcggtgatca tcgctgcaca caatgaggag tcgtgcattg ctgaaaaaat
     9421 tgagagcatt tatagcggaa cttaccctta cgacaggctg gaagtgctgg tgggatcgga
     9481 taattcggac gaccggacgg tggaaatttt agcggcactc caaaagaggt ttccaaccct
     9541 tcaatttttc gacttccggg aaaggagagg gaaaggaagc gtggtgaatg acctggttgg
     9601 cgcgtcaaaa ggttctttgc tcgttttaac ggatgcaaat gtcatgttcg acagggagac
     9661 gctgttctat cttgtccgcc atttcaggaa tcccgcgata ggactcgtgg acacgaatat
     9721 ggtgaatatg gggatgaaaa aagacgggat atcttaccag gagcgggctt atatttcgag
     9781 agaggtgagg atcaaagata tggaaggccg tctctgggga gcgatgatgg gcccgttcgg
     9841 cggatgttac gctatccgga gggaagatta ctgccaggta cccgccaatt tcctggtgga
     9901 tgatttttat atcaatatga ccatctttga gaaaggtaaa ctggccgtca atgacttgaa
     9961 ttcgatcgtt tttgaaaata ttccggatga actgaaagtt gaattccgga gaaaagtgcg
    10021 tatcggcacg ggtaattttc agaaccttag gcgtttctgg aggctggcat tcacgatgcg
    10081 ttacggtttt gcctttattt cacacaaaat tatccgctgg ttcggcccgt ttttattgct
    10141 ggcagcgctt gttgccaata tttacctggc tgtattcagt agagtttacc tgttcatatt
    10201 ggccggtcat ctgctggttt acttgctacc gttttttgac cgtttnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnaagaa aaggatcgaa ataacggtcg gcaggcccag
    10381 ccccccttta cggataatag cgcccagcgg ggcaccgata aaaaacatca ccaggcaggc
    10441 gaaggaaagg gtgaatttcc ggtgccagac gatcttatgt ttgaccagtg attcgttgct
    10501 gacacggaag gcatccgact gaaaatccag gttctttttg atgcccctgg ccgaggtcaa
    10561 cgcattggaa acgatccgga tttgatcatc agtggaaaaa ccgctgagaa aatccccggg
    10621 cacaaccgtc actgaatcgg tggagggcac gtatgccgtg tcgttattga gatatagctg
    10681 gtaatttctc gggaagttac gggcgaggtt ctcctttcgt tcgaccagcg accggcttag
    10741 ggaatcgatg aaataattga gctgaccgat gttcagcatc tggtaattgt tcttgaaaag
    10801 ctcttcattt gtccggttca gctcgaatgc cgacaaatcg aatttacgtt gctggatttt
    10861 gaaggaagta cgctgaaaag gctgttggtt tttggagtct ttctggtcaa ctatgtcctg
    10921 gtaattaaag ccgtcataca gcgtaaagat caggtaccgg ccatcgggtg tcgattccat
    10981 gatccccgaa tcagccacgg tcacatcaat atttcccctg ttttcggtgt ggttgtaaat
    11041 cattacttta tagatcctgt ttccgtcttt accctttttt cccacacgga tcacataatc
    11101 ctccatatca ttgtaaaaga ttccttcccg gatattcacg gccagctttt tcttccggat
    11161 atcgtaaagc agcgatttga acttgaggtt ggccaccggc agcacattgt tcgaaaaata
    11221 aaatgcaaac gcggaaatac ataacgaaag gatgatcagg ggcttcatga tcgaccgcag
    11281 ggagatgccg gccgacttca tggcaaccag ttcataatgc tcgccgaggt tgccgaatgt
    11341 catgagcgac gacagcagga tggctaaggg gagggctagt ggaacaaatg taaatgaagc
    11401 ataaaagagc agttcggcaa tgacatacca ctccaggccc tttccgacaa ggtcgtcgat
    11461 atacatccac atgaactgca taagaagaat gaacagggcg ataaaaaagg tcaccaatag
    11521 cgggcctgaa taggttttaa ggaccagtgc gttgatcttt ttcattttac actttagact
    11581 ctaaacgcaa aggtaagcgt ttaattgcca cttaaaaaat tagagaatgg ttaggaatat
    11641 tccattttta tgttattttt gcacttcagg cgaaaccaaa tgtttaatta aataaaggaa
    11701 aaaaatgaaa aaactattac tcattgccat ggctttgatt atcggcgtcg tggcttattc
    11761 acaaaacaag gttcagctta gcaaggagat gcgtgaacag accgtcaccc tgaccaggca
    11821 aaatgatatt gctgaagaga tcgttttcgg caatttctct gatcctacgg tcagcaacag
    11881 caaagctgcc ccgatcgaaa ccctcatcgg cacgacaaag tacgacctga tgacgaactc
    11941 aatgattcag aaccgtcttt acctgcatgc cgacaatacc atcggggctg tgtggacgat
    12001 gggtctgggt accgagcccg ctttctcaga cagaggcacc ggctacaact attatgacgg
    12061 agcccagtgg ggtgatgcac cgaccgtaag gattgaaccc gaaaggaccg gatggcctgc
    12121 ttacgcaccc tggggtccga acggtgagat tattgccgcc cata
//
LOCUS       DAOPNH010000067        12031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5554_length_12031_cov_6.309703, whole genome shotgun sequence.
ACCESSION   DAOPNH010000067 DAOPNH010000000
VERSION     DAOPNH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_12031_cov_6.309703"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKZ74_05100"
     CDS             <1..1003
                     /locus_tag="PKZ74_05100"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPM92170.1"
                     /translation="NDVVRSLIFVLLAFGLLWAYLKGKAKLAYVLALLPLFVIVDMWP
                     VNKRYLNNDDFIKKNKVDNPYQATPADIAILKDKDLSYRVYNMNEAFDASARTSYFHK
                     NIGGYHGAKMRRYQEVVDHALMDERRNIAEALGNSQAAPGEAMAGATAFNMLNTRYLI
                     LNANSEPLKNPYACGNAWFVGDYKIVADADEEIASLNGLDPARTAVIDKRFESFVAGF
                     RPSAEPSGNIDLLSYSPNQLVYEYDAPADQLAVFSEIYYDKGWNAYLDGRKSPHFRAN
                     FILRAMILPAGQHKLEFRFEPKSYYLGGNISLASSLILLLLIAGYFAKEFFINRKKA"
     gene            1000..2328
                     /locus_tag="PKZ74_05105"
     CDS             1000..2328
                     /locus_tag="PKZ74_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM92171.1"
                     /translation="MKKVLVILYYWPPAGGSAVQRWLRFCKYLPEYGWEPTVVAPENA
                     FYTDLDKELVAEIPEKLNVLKIPIREPHRIYKAFVGLKKEENIGAYMSMKTEPKGFAR
                     IKNDLSVWIRSNFFVPDARIFWIRPVTRFLKKYLASHTFDAIVTTGPPHSIHLIGLKL
                     KASTTIPWVADFRDPWTSTDLHSALKLTRYAEKKHIRLESKVIRAADHIIAIGNDMKN
                     EFLKIGAENITVITNGYDESDIRAEYTERDRKFSIVHLGTLPATRNSSALWKVLSEKV
                     VTDKQFAKDLSIHLIGTVDYKVVQEIEALNIKDYLSITQFVKHNEGMEILRRSAVLLL
                     LINRSKNARGVVTGKIFEYMAVRRPVLLIGPVDGDAAAIIRETECGLCAEFDDEQMIR
                     TLIDHYYNLFQKNELEARPGKVSQYSRKSLAATLAQLLDKLQGDISNQVN"
     gene            2451..3044
                     /locus_tag="PKZ74_05110"
     CDS             2451..3044
                     /locus_tag="PKZ74_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM92172.1"
                     /translation="MMEIWPAVAERYLDAGCGFGLKTYIFSEYFQHSTGIDFSHNVAE
                     ICKILNNKPDSLEFQITDIENLEGVGYDLITAFGLSYFNISDMGILAARIEKQVDKLL
                     NRGGVFLITTRTGPSSNAGTGWHLLNKTEIKVVKHTLRNNLRDCRIDLFEPDKSWTYL
                     RSGKFLQVSGSVYKTLMNKPRDLFIIIRKDGPDAGIK"
     gene            3019..3687
                     /locus_tag="PKZ74_05115"
     CDS             3019..3687
                     /locus_tag="PKZ74_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM92173.1"
                     /translation="MDRTQVLNSFRKRPVSGKTDFITNYCKGKRVLDIGCIGQDNNPD
                     RPEWLHGKIRQVATLLAGVDINTEKMEQLREQGYLLFSAITFSDKDFKDPDIVVMADV
                     IEHVSDIVAFLKHYKKLASDRTVFLISTPNPYSIRQSFSILLFGRPGINPEHTVAIDP
                     TNMMELLDRAGFEFIDFSWLHEYDRPKKSYNKVLFQIYRLMYSVRKFWAPNYIVAVKP
                     NKPG"
     gene            3963..4760
                     /locus_tag="PKZ74_05120"
     CDS             3963..4760
                     /locus_tag="PKZ74_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92174.1"
                     /translation="MKVIYETIDNMVHLNFHYISGELPFLKPAGPLLLRHYAALEKKL
                     AAMADGVIVNSAALQEYFRPYRTSLIFYTSPFENIVQVENKSKPAALLYLGLFTRNKG
                     ATETLALQADLGLPLFIFGDTSDKNLINAIKSNPEVTWESRIAPDVLAHKLSRLTERY
                     RLMGCSIIHPVHFSYATQEANKDQDYLAMGLPIIGNDRAPTLEKIRAGCGILFNEKNK
                     IDEVIENPEKYQLLQNNCLTFYNQHYSRERFKENLLRLVDHISGQRM"
     gene            complement(4736..6037)
                     /locus_tag="PKZ74_05125"
     CDS             complement(4736..6037)
                     /locus_tag="PKZ74_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92175.1"
                     /translation="MNIKGIDIAKYLGENFNHLYRSDAGIIEFIAGSLPENISYLQIL
                     NEIITQPKPLFIDFNGIPLPLFYYESADHKITLADLTVKDLSINSFLLLSGWQEVVIK
                     KRDLHERFPYSESLQSKFNFAGIPVVNFLFNEMCLRIKQSGYHCNPLKFSTAGSNIIF
                     THDIDRLYSGWYEATGHVIKNPWTGKLRSWVELSRRKLFFQVDDYQDACYRLLALLDK
                     FQIKSVFFFLASRSRDDADYRLGDHFTVRFIKDLQEAGHIPGLHAGYHTFRDPGQLNR
                     QIGQACNFLGENIINNRQHFLKFDLSFTPLTLFNAGIKYDFSLGFAEAPGFRNSIATP
                     FYVFDFLHPESARLVEVPLFFMDGTYSHYQRIDKHGFRNPLEDIAQYSKYFNMNFSVL
                     FHNSVFTDFKYQGFSQLFIDVAEFAAEQGFSADAHIRWPEM"
     gene            complement(6034..7512)
                     /locus_tag="PKZ74_05130"
     CDS             complement(6034..7512)
                     /locus_tag="PKZ74_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM92176.1"
                     /translation="MFFYSSFAKKSVFYTFLQILPALAGIIILRIYSSLLTREEYAVL
                     AMLTTTSTLSLIINSLCMDQVITRYYYDHVHDRKKLDEFFLSVISVMVALLFVIIAFV
                     FIIPQGINEYLFKGMLENMQWQFIASVLVGFALSLYKSLLSFQRNEQAHFKVLILTLS
                     ASVLQVAFVWIGIRFFQNSVLGAQLGKFAGIALPTIVLIFMYFAGRKASIKFALIREV
                     VPFFAPLVLYGLMYWGVSQFDQVLFQMRMQSLELLALYVMAFNIALFADLILSGLSSF
                     IIPEMNMIMKQQTDTGRISNYIHLFVLMSCFSILAVSLAGNLMTDFYLDPKYGSIGWI
                     IDGLLIGYIFRMLYTIYSFPVYYQKKTMILTIALGISLAISLSVNWFLIPSAGIFALL
                     LSNFLSRFSQAWLTAGLARKMSTIPFNRMKVFGLTVIFVLLLLSLMIIGYFTRVNHYL
                     AISAAFIIAVLTALILFRKQLPVLISLLRDGFLKNRHENLKH"
     gene            complement(7553..8842)
                     /gene="eno"
                     /locus_tag="PKZ74_05135"
     CDS             complement(7553..8842)
                     /gene="eno"
                     /locus_tag="PKZ74_05135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPM92177.1"
                     /translation="MSAIIDIHARQILDSRGNPTIEVDVFTESGVIGRAAIPSGASTG
                     IHEAVELRDGDKEYLGKGVLKAVHNVNNVIKEEIVGYNVTDQGDIDQHMIELDGTPNK
                     AKLGANAILGVSLAAAKAGAMETGQYLYRYVGGVNANTLPIPMMNILNGGSHADNSID
                     FQEFMIMPAGAESFSQALRMGAEVFHTLKGVLKKKGYSTNVGDEGGFAPNLGSNEEAI
                     QVVLEAIEKAGYKPGEDIYIALDPASSEYFLTDEKVYHLKKSSGEKLTPAQMVDYWAE
                     WVRKYPIISIEDGMAEDDWEGWKLMTDKVGSKIQLVGDDLFVTNTNRLQEGISKGVAN
                     SILVKVNQIGTLTETINAVNLAYTNAYTAVISHRSGETEDVTIADLAVALNTGMIKTG
                     SASRSDRIAKYNQLLRIEEKLGNSAYYPGKSFKFCKK"
     gene            complement(9012..9665)
                     /gene="rplQ"
                     /locus_tag="PKZ74_05140"
     CDS             complement(9012..9665)
                     /gene="rplQ"
                     /locus_tag="PKZ74_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPM92178.1"
                     /translation="MRHGKKINHLSRKSAHRKAMLSNMATSLIMHKRITTTLAKAKAL
                     RVYVEPIITRSKEDTTHSRRVVFSYLRSKEAVTELFRDVSAKVADRPGGYTRIIKLGL
                     RLGDNAEMAMIELVDFNLNLLGEKAQKKAKTTRRGRRGGAKKAGAEEVADTVAAPAKP
                     AKAKAKKEEEPVAVAAPAPQSVPAEMPEPEAEPVVVADAELPAEEILPEEAQPADET"
     gene            complement(9672..10664)
                     /locus_tag="PKZ74_05145"
     CDS             complement(9672..10664)
                     /locus_tag="PKZ74_05145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPM92179.1"
                     /translation="MAVLAFQKPEKVIMVETDTSKGVFEFRPLEPGFGVTIGNALRRI
                     LLSSLEGFAITSIKIDGVSHEFSTITGVVEDVSEIILNLKKVRFKRQIETENSEKVTV
                     VIGDQDSFKAGDINKFLSVFQVLNPEVEICHLDPSTKLTIEINIEKGRGYVPSEENKV
                     MNTDINMIFIDSIHTPIRNVKYKIDNYRVEQKTDYEKLTLEIETDGSIMPKEALKEAA
                     KILIHHFMLFSDEKITLDTEEKAVAEEFDENSLHIRQLLKTKLVDMDLSVRALNCLKA
                     ADVETLGDLVQFNKNDLLKFRNFGKKSLTELEDLVKTKGLEFGMNVAKYKLDKE"
     gene            complement(10703..11308)
                     /gene="rpsD"
                     /locus_tag="PKZ74_05150"
     CDS             complement(10703..11308)
                     /gene="rpsD"
                     /locus_tag="PKZ74_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPM92180.1"
                     /translation="MARYRGPKSKIARRFREPIFGPDRVLDKKPYPPGIHGSSHRRKK
                     ESEYGVQLAEKQKAKYTYGILERQFRKTFAMATRKKGITGEVLLQLIEARLDNVVFRL
                     GIAPTRRAARQLVTHRHIVVNGEVVNVPSYSLKEGDVVGVREKAKSLEVISESLASRS
                     NKFSWLDWNDEKKEGKFMNYPPREEIPENIREQLIVELYSK"
     gene            complement(11341..11730)
                     /gene="rpsK"
                     /locus_tag="PKZ74_05155"
     CDS             complement(11341..11730)
                     /gene="rpsK"
                     /locus_tag="PKZ74_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPM92181.1"
                     /translation="MAKTTRATKKRIVKVEPVGRAYIHASFNNIIITLTNNAGQVISW
                     ASAGKMGFKGSKKNTPYAAQMAAEECSKTAFDLGLRKVKVFVKGPGSGRESAIRTIHT
                     AGIEVSEIQDVTPLPHNGCRPPKRRRV"
     gene            complement(11749..12031)
                     /gene="rpsM"
                     /locus_tag="PKZ74_05160"
                     /pseudo
     CDS             complement(11749..12031)
                     /gene="rpsM"
                     /locus_tag="PKZ74_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
BASE COUNT         3228 a         2691 c         2735 g         3377 t
ORIGIN      
        1 caatgatgta gtcaggtctc tgatctttgt tctgctcgcc ttcggtttgc tctgggccta
       61 ccttaaaggt aaggccaaac tggcctatgt gctggcactc cttcccctgt ttgtgattgt
      121 ggatatgtgg ccggtaaaca aaagatatct gaacaatgat gatttcatca agaaaaataa
      181 agttgataat ccataccagg ctactccggc tgatattgct atcttaaaag acaaagacct
      241 gtcgtacagg gtctataata tgaatgaagc cttcgatgca tcggcccgga catcctactt
      301 tcacaagaat atcggaggtt accatggagc caagatgcga cggtaccagg aggtggtcga
      361 tcatgcgctg atggatgaaa ggcggaatat tgccgaagca cttggaaata gccaggctgc
      421 acccggagaa gcaatggccg gcgccacagc attcaatatg ctgaacacga ggtatcttat
      481 cctgaatgca aacagcgagc cgctgaaaaa tccatacgca tgcggaaacg cctggtttgt
      541 cggtgattat aaaatagtgg ccgatgccga tgaagagatt gcttcgctca atggcctgga
      601 tcctgcaaga acggcggtca ttgataaacg gtttgaatcc tttgtggccg gtttcagacc
      661 gtcggcagaa ccttccggga acatagatct actatcctac tcccccaatc aactggttta
      721 cgaatatgat gcacctgcgg accaactggc cgttttctca gagatttatt atgataaagg
      781 atggaacgct tacctggatg gcagaaaatc accgcatttc agggctaatt tcattctgag
      841 agccatgatc cttcctgccg ggcagcataa acttgaattc aggtttgaac cgaaatctta
      901 ttatttagga ggaaatatct ccctggcaag ttcattgata ttattacttt taattgcggg
      961 ctattttgca aaagaattct tcataaatag gaagaaagca tgaagaaagt cctggtgatt
     1021 ttgtattact ggccacctgc cggcggaagc gccgtgcaaa gatggctcag gttctgcaaa
     1081 tatttgcctg aatacggttg ggaaccaacc gtagtagccc ctgaaaatgc tttttatacc
     1141 gatctggata aagaactggt tgcagaaatc cccgaaaagc tgaatgtcct taagatcccg
     1201 atccgtgagc ctcaccgaat atataaagct tttgtgggat tgaagaaaga agaaaatatc
     1261 ggggcgtata tgtcgatgaa aacagagccg aagggctttg ccaggataaa aaatgatctg
     1321 tcggtctgga tccggtctaa tttcttcgtt ccggatgccc gaattttctg gataaggcct
     1381 gtcaccagat ttctgaagaa ataccttgcc tcacatacct tcgatgcaat tgtaacgacc
     1441 gggccgcctc atagtattca cctgatcggg ttaaaactca aagcttcaac tacgatcccg
     1501 tgggttgctg atttcagaga tccctggacg agtaccgatt tgcactccgc gctgaaattg
     1561 acacggtatg ctgaaaaaaa gcatatcagg ctggaatcga aggtcattcg agctgctgac
     1621 catatcattg ccatcggaaa tgatatgaag aacgagtttc tgaagatcgg agctgaaaat
     1681 atcacggtga tcacgaatgg atatgatgaa tcggacattc gtgcagaata tactgaaaga
     1741 gatcgaaaat tctccattgt tcatcttgga acactgcctg caacccggaa ctcttccgca
     1801 ttatggaagg ttctgtccga aaaggtcgta accgataagc aatttgcaaa agatttatca
     1861 attcatttaa tagggaccgt tgactataaa gtggtccagg agattgaagc ccttaacata
     1921 aaagactatc tcagcatcac tcaattcgtg aaacataatg aaggcatgga aattctgcga
     1981 agatcagccg tactgctgct cctgatcaac cggtcgaaaa atgcaagggg cgtcgtaacc
     2041 ggcaagatat tcgaatacat ggctgtccgg cgccctgttt tactgatcgg gcccgttgac
     2101 ggcgatgctg cggccatcat ccgggaaacg gaatgcggtt tatgcgcaga gtttgatgat
     2161 gaacagatga ttcgtacttt gatcgatcat tattacaacc ttttccaaaa gaatgaatta
     2221 gaagccagac cggggaaagt gagccagtac tcgcgtaagt cgctggcagc caccctggct
     2281 caattgcttg acaaattgca aggtgatatt tcaaatcagg tgaattaaat aaacaggaca
     2341 ggccattgga tataaaaatt ttttacgatc agaaatacgc tacatttgaa tctttgccgg
     2401 gatttcaagc agaacgggaa tattgcatcc gtattattca atggatgaag atgatggaaa
     2461 tctggccggc ggtagctgaa aggtatctgg atgccggttg tggattcggg cttaagactt
     2521 atattttctc agaatatttt caacattcca caggtattga tttcagccac aatgttgcag
     2581 aaatttgtaa aatactgaat aacaaaccgg attcactgga gtttcagata actgacattg
     2641 aaaacttaga gggtgtcggt tatgatctga taaccgcgtt tggtttgagc tattttaata
     2701 tttcagatat gggtattctg gctgccagaa ttgaaaaaca ggtcgataaa ctgctgaaca
     2761 ggggtggtgt ttttctgata actacccgga caggtccgtc atcgaacgca ggaaccggat
     2821 ggcacctttt aaataaaact gaaataaaag tcgtaaaaca tacgttgagg aacaacctga
     2881 gggattgccg gattgatctt tttgaaccgg ataaaagttg gacttacctt aggtccggca
     2941 aatttttgca ggtatcagga tcggtctaca aaacactgat gaacaagcca agggatttgt
     3001 ttataataat tcggaaagat ggaccggacg caggtattaa atagctttcg gaagcgaccg
     3061 gtttccggca aaacggattt catcacgaac tattgcaagg gtaaacgggt tcttgatatc
     3121 ggatgcattg gccaggacaa caatcccgac cggccggaat ggctccatgg caagatcagg
     3181 caggttgcca cattgttagc cggtgtcgat atcaataccg aaaagatgga acagctccgg
     3241 gaacagggtt accttctgtt ttctgccatc accttttccg acaaggattt caaagatccc
     3301 gatattgtgg taatggcaga tgttattgaa catgtcagcg atattgtcgc atttttaaaa
     3361 cattataaaa aattggcatc cgatcgaacg gtttttctga tcagtacgcc aaatccatac
     3421 agtatccgtc aatccttcag catcttgtta ttcggacgcc cgggtattaa cccggaacat
     3481 acagtagcta ttgatccgac caatatgatg gaattattgg atcgagccgg atttgaattt
     3541 atagatttct cctggctgca cgaatatgac aggccaaaaa agagctataa taaagtgttg
     3601 tttcaaattt accggttaat gtattctgtc agaaagttct gggctccgaa ttacatagta
     3661 gcggtaaaac cgaataaacc gggatgagaa aaattacgat tgtcactgct tttcccatat
     3721 cgatcccgca tccgcgggtt gagaccgaga aaattattct gtctgaagca ggatacgagg
     3781 tctcgcattt atcttatccc cggcggcctg atatttttca acggttaatc aattattctt
     3841 catttaatta ctttaaaaaa catctttata aagaaatact ggaaagcata ccccgggatg
     3901 gaatcgtaat tttatatgat ttccacttac ttcctttggt taaggccctt aaaaaaagac
     3961 aaatgaaagt catatatgaa accatcgaca acatggtcca tctgaacttt cattatattt
     4021 cgggcgagtt accattcctg aagccagccg gaccgttgct actaagacat tatgctgccc
     4081 ttgagaaaaa actggctgcg atggctgatg gcgtgattgt caattcggcg gccctgcagg
     4141 agtacttcag gccatatcgt acttccttga ttttctatac ttcacctttt gaaaatatcg
     4201 ttcaggtcga aaataaatcg aagccggcag ctttgcttta tcttggctta ttcacccgaa
     4261 acaagggcgc aacagagaca cttgcgctgc aggcagacct tggcctccct cttttcattt
     4321 ttggtgatac ctctgataaa aatttaataa atgctatcaa atcgaatccg gaagttacgt
     4381 gggaatcaag aatcgccccg gatgtcctgg cccataagct ttcgcggctg acagagaggt
     4441 acaggttgat gggttgcagc atcatccatc ccgtccactt cagttatgcg acacaggaag
     4501 ccaacaaaga ccaggactac ctggcgatgg gtcttcccat catcgggaat gacagggctc
     4561 ctacattaga gaaaatcagg gcaggatgcg gcatcctctt taatgaaaaa aataaaatcg
     4621 atgaggttat agagaacccg gaaaagtatc agctgcttca aaataactgc ttgacttttt
     4681 acaatcagca ctattcgcgg gagagattca aagagaacct gctccgactg gtggatcaca
     4741 tttcaggcca acgtatgtga gcatctgctg aaaaaccttg ctcagcagcg aattcagcaa
     4801 cgtctataaa aagctggcta aaaccctggt atttaaaatc ggtaaaaacg gagttatgaa
     4861 acaataccga aaaattcata ttaaagtatt ttgaatactg agctatatct tccagtggat
     4921 ttctgaatcc gtgcttatct atacgctgat aatgcgaata ggtgccatcc atgaagaaca
     4981 gcggtacctc cacaagccgg gcagattccg ggtgcaagaa atcaaaaaca tagaacgggg
     5041 tggctatact gttccgaaat ccgggagctt cagcaaatcc aagcgaaaaa tcatatttta
     5101 taccggcgtt aaaaagcgtc agtggcgtaa aagacaagtc aaacttaaga aaatgctgac
     5161 ggttattaat tatattttcc ccaaggaaat tacaggcctg cccgatttgc cggttcaact
     5221 gtccgggatc ccggaaagta tggtaacccg catgaagtcc gggaatgtga ccagcctcct
     5281 gaagatcctt gataaacctg acggtaaaat gatctccgag acgataatcg gcatcgtcgc
     5341 gggaacgact ggcaagaaaa aagaaaaccg acttgatctg aaatttatcc agcagcgcca
     5401 gtaacctgta acaggcatcc tggtaatcat ctacctgaaa aaataatttc ctgcggctta
     5461 actctaccca tgatcttaat tttccggtcc acggattttt tataacatga ccggttgctt
     5521 cataccaccc ggaatataat cggtctatat cgtgagtaaa aataatattt gagcctgccg
     5581 tagaaaattt taaaggatta caatggtatc cggactgctt tattcgcagg cacatctcgt
     5641 taaacaagaa attgacgacc ggaatcccgg caaaattaaa ttttgactga agcgactctg
     5701 aatatgggaa tcgttcatgc agatcccgct tttttataac tacttcctgc catccgctaa
     5761 gcaaaaggaa ggaattaata gataaatcct taactgtcag atcggccaga gtgattttat
     5821 gatcagcaga ctcataatag aataatggca agggtattcc gttaaagtcg ataaacaaag
     5881 gttttggctg agtaattatt tcgttcagaa tctgcaggta gcttatattc tccggtaacg
     5941 atcctgcgat aaattctatg attcctgcat ccgatcggta caggtggtta aaattttccc
     6001 cgaggtattt tgcaatatcg atccctttta tattcaatgc tttaaatttt catgcctgtt
     6061 tttaagaaat ccatctctga ggaggctgat cagtactggt aattgtttcc ggaacaagat
     6121 caatgcagta agaaccgcaa taataaatgc agcggaaata gccaaataat ggttgacgcg
     6181 ggtgaaatac ccaattatca tcaggctaag taacaaaaga acaaaaataa ctgtcagtcc
     6241 gaatactttc atcctgttaa acggtatggt tgacatttta cgtgcaagcc cagccgttag
     6301 ccaggcctgg gaaaaacgag agaggaaatt ggaaagcaaa agggcaaaaa ttcctgcgga
     6361 agggatcaga aaccagttta cggataaact aatcgccagg gatatcccaa gggctatggt
     6421 taaaatcatt gtttttttct gataataaac cggaaagcta taaatggtat acaacattct
     6481 gaagatataa cctataagca gaccatcaat aatccatccg atagaaccat atttaggatc
     6541 cagataaaaa tctgtcatca ggtttcctgc gagggagaca gcaagtatag aaaaacaact
     6601 cattaagacg aaaagatgga tataattgga tatccttcct gtatcggttt gctgtttcat
     6661 gatcatattc atttcaggaa taatgaaaga ggacagtcca cttaagatta ggtcggcaaa
     6721 taatgcgata ttaaatgcca tcacgtataa tgccagtaat tccaggcttt gcatgcgcat
     6781 ttgaaacagt acctggtcga attgtgacac tccccaatac atcagtccat ataataccag
     6841 cggtgcaaaa aaaggaacaa cctccctgat aagtgcaaat tttattgagg ccttccttcc
     6901 tgcaaaatac atgaatatca atactattgt tggcagggca attccggcaa acttgcccaa
     6961 ctgtgcgccg agtacactat tctgaaagaa acggataccg atccagacaa aagcgacttg
     7021 aagaacagac gcagaaagtg tcaggatcaa cactttaaaa tgagcttgtt cgtttcgctg
     7081 aaaacttaac agggatttat acaaagaaag agcaaatccg accagtaccg aggcgataaa
     7141 ctgccactgc atattttcta acatcccttt aaaaagatat tcattgatac cctggggaat
     7201 aatgaatacg aaagctatta tgacgaaaag caatgccacc atgacagata tcacagaaag
     7261 aaaaaattca tccaattttt tcctgtcatg cacatgatca taatagtacc ttgtgatgac
     7321 ctgatccata catagggagt ttatgatcag gctgagagta gaagtggttg taagcatggc
     7381 cagaacggca tattcctccc tggtaagcag ggaggaatag attcttaaaa ttattatgcc
     7441 tgccaatgca ggaagaatct gcaggaaagt atagaatacc gatttcttgg cgaaactgct
     7501 ataaaaaaac acaggttttc agattattat cacaacgata aaagttccga gtttattttt
     7561 tacaaaactt aaaacttttg cccggataat aagccgaatt tcccaatttc tcttcgatcc
     7621 ggagcaactg gttgtatttg gcgatcctgt cggaacggct ggcgctcccg gttttgatca
     7681 ttcccgtatt cagggccacg gccaggtcgg cgatggtaac gtcttcggtt tcgcccgaac
     7741 ggtggctgat gacggctgta taagcattgg tgtacgccag gttcacggca ttgatcgtct
     7801 cggtcagcgt accgatctgg ttgactttta ccaggatgct gttggccacg cctttgctga
     7861 tgccttcctg caaacggtta gtgttggtca cgaacaggtc gtcgcccacg agctggattt
     7921 tgctccccac tttgtcggtc atcaacttcc agccttccca gtcatcttcg gccataccgt
     7981 cttcgatcga gatgatcggg tacttcctga cccattcggc ccagtagtcg accatttggg
     8041 caggagtgag cttttcgccg gaggatttct tcaggtggta tactttttca tccgtcagga
     8101 aatactccga agatgccggg tcaagggcga tatagatatc ttcgcccggt ttgtatcccg
     8161 ctttttcgat ggcttcgagc actacctgga tggcttcttc gttgctgccc aggttcgggg
     8221 caaaaccgcc ttcgtcgccg acattggtcg aatagccctt tttcttgagg acgcctttga
     8281 gcgtatggaa tacctctgct cccatccgga gcgcctggct gaaactttcc gccccggccg
     8341 gcatgatcat gaactcctgg aaatcgatgc tgttatcggc atgtgaacca ccgttaagga
     8401 tattcatcat cggtataggc aacgtattgg cattcacccc gccgacatac cggtaaagat
     8461 actggcccgt ttccatggcg cctgcttttg cagcggccag cgaaacgccc aggatggcgt
     8521 tggcgcccag tttggccttg ttcggggtac catcgagttc gatcatatgc tggtcgatat
     8581 cgccctggtc cgtcacattg tatccgacga tctcttcttt tataacatta ttcacattgt
     8641 gaacagcttt caggacacct tttccaaggt attctttgtc accgtcgcgc agttcgacgg
     8701 cttcgtggat cccggtcgat gcgcccgagg ggatggcggc ccttccgatg acgcccgatt
     8761 cggtaaaaac atccacttca atggttggat tgccccgtga atccaggatc tgacgggcat
     8821 ggatatcgat tattgcactc atatttttaa ttcttaattt ttaattcttg attcttaatt
     8881 atctataaga ttgagatgtt ttatgaaaac tttaaactaa aaaaggataa agcgcttcca
     8941 ccttatcctt tccattgata tttcatgtaa tgttaagctt ttgtttcttc ttcgggttcc
     9001 ggatcttccg gttaggtttc atcagccggc tgtgcctcct cgggcagtat ttcttctgcc
     9061 ggtagttccg catcagctac cacgaccggt tctgcttccg gttcgggcat ctctgcgggg
     9121 acactctggg gtgccggggc tgcaacggct accggttcct cttctttctt tgctttggct
     9181 ttggctggtt tggccggggc tgcaacggta tcggccactt cttcggcgcc ggctttcttt
     9241 gctccgcccc ttctgccacg acgggtcgtc ttggcctttt tttgagcttt ctcacccagc
     9301 aggttgaggt tgaagtcaac cagttcgatc atcgccattt cagcattgtc gcccagcctc
     9361 agtcccagtt tgatgatcct tgtgtaacca cccgggcggt cggctacttt ggcagagaca
     9421 tccctgaaaa gttccgtgac agcttccttg ctccgcaggt agctgaacac cactcttctg
     9481 gagtgggttg tatcctcctt tgaacgggtg atgatcggct cgacgtacac cctgagggct
     9541 ttggccttcg ccagggtcgt cgtgatccgt ttatgcatga tgagcgatgt tgccatattc
     9601 gacagcattg ccttccggtg agcgcttttt ctactcaggt gattgatttt ctttccgtgt
     9661 ctcatcgcga tttattcctt atcaagtttg tattttgcaa cattcattcc gaactcaagt
     9721 cctttggtct taaccaggtc ttccagttcg gtcagcgact tcttcccgaa gttcctgaat
     9781 ttgagcaggt cgtttttatt gaactgcacc aggtcgccga gtgtttccac gtctgcagct
     9841 ttcaggcagt tgagtgccct gacgctgagg tccatatcta ccagtttggt cttgagcaac
     9901 tgccggatgt gcagcgaatt ctcatcgaat tcttcggcta cggctttctc ttccgtatcc
     9961 agggtgattt tttcatccga aaagagcatg aaatggtgga tcagaatctt agctgcttct
    10021 ttcagcgctt cctttggcat gatggatcca tcggtttcga tctccagggt cagcttttcg
    10081 tagtcggttt tttgttccac acggtaatta tcgatcttgt atttcacatt ccggatggga
    10141 gtatggatag aatcgataaa tatcatgttg atatcggtgt tcatcacttt attctcttcg
    10201 ctgggaacat aaccgcggcc cttctcgata ttgatctcga tggtcagttt ggtagagggg
    10261 tcgaggtggc agatttccac ttccgggtta agcacctgga aaaccgataa gaacttgtta
    10321 atatcgccgg ctttgaatga gtcctggtca ccgatcacga cggttacttt ttcgctgttt
    10381 tccgtctcaa tctgacgttt gaaccggacc tttttgaggt tcaggataat ttccgaaaca
    10441 tcttccacca ctcctgtaat ggtagaaaac tcatgtgaaa cgccatcgat cttgatggac
    10501 gtaatggcaa aaccttcgag ggacgatagc aatattcttc ttaatgcatt tccgatcgtc
    10561 acaccgaaac cgggctccag gggtctgaat tcaaacacac cttttgaggt gtcggtttcg
    10621 accatgatga ctttttcggg tttttggaat gctaaaactg ccatgttcgt ttttttataa
    10681 ttaaaagaaa aaattcagat gattatttgg agtacaactc gacgatgagc tgttcccgga
    10741 tattttcggg tatttcctcc ctgggaggat agttcatgaa tttgccctcc tttttttcgt
    10801 cattccagtc gagccatgag aatttattgg agcgtgaagc aagcgattct gagattactt
    10861 cgagcgattt ggctttttcc ctgacgccga cgacatcacc ttctttgagt gaataggaag
    10921 ggacgttgac cacttctccg ttgacgacaa tatgtcggtg ggtaaccagc tggcgggcag
    10981 cacgacgggt tggggcgatg cccagtcgga acacgacgtt atccagacgc gcttcgatca
    11041 attgcagcag aacttcaccg gtgataccct ttttccgggt agccatagcg aaggtttttc
    11101 tgaactgccg ttccaggata ccataggtat atttggcttt ctgtttttct gccagctgga
    11161 cgccgtactc agactccttt ttcctgcggt gtgacgaacc gtggatgccc ggagggtaag
    11221 gttttttgtc cagtacccgg tccggtccga aaatcggttc cctgaatctt ctcgctatct
    11281 ttgatttagg gcctctgtat cttgccattt cttatattga ttaaattatt cttaatacga
    11341 ttatactctt cttcttttgg gcgggcggca tccgttatgc ggaagcgggg tcacatcctg
    11401 tatctcggat acttcgatcc cggcggtatg aatggtacgg atagccgatt cgcgtcctga
    11461 acccggtcct ttcacaaaca cctttacttt cctgagaccc aggtcaaaag ctgtcttgga
    11521 acactcctcc gcagccattt gggcagcata aggagtattt ttcttggaac ctttaaagcc
    11581 cattttaccg gcagatgccc aggagatgac ctgcccggca ttgttggtca gggtgatgat
    11641 gatattatta aaagatgcgt gaatataagc ccttccgact ggctcgacct ttacgatccg
    11701 cttctttgtt gctctggttg ttttagccat ttttactgaa aattatgatt aacctttagg
    11761 tgctttcttc ttgttggcaa cggtcttttt ctttcccttg cgggtacggg cattgttctt
    11821 ggtgctctgt cccgcgacgg taatccgaga cggtgccgga tgccacggta ggtcccaatg
    11881 tccatgagac gtttgatatt aagctggact tccgaacgaa gcgcaccctc gaccttaaac
    11941 tcattattga tgatcgtacg gatgttgttc tgttcttcat cgctccagtc ctggaccttt
    12001 ttgttcaggt caacgccggc tttctcaagg a
//
LOCUS       DAOPNH010000068        11963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5615_length_11963_cov_5.253611, whole genome shotgun sequence.
ACCESSION   DAOPNH010000068 DAOPNH010000000
VERSION     DAOPNH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5615_length_11963_cov_5.253611"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="PKZ74_05165"
     CDS             complement(<1..1544)
                     /locus_tag="PKZ74_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPM92182.1"
                     /translation="MNRLYNCVFSILAIVTGLAGCNPEPEQVQPLEVALKSNWSFRQM
                     ETGPWYKAVVPGTVHTDLLLNNIIEDPYYRLNEKQLQWIDKKDWEYRTSFYVDKDVLN
                     NDRVELDFEGLDTYARVFLNGSLILEADNMFRRWTADVKGKIRAGDNELVVLLESPTR
                     KGLEALSAYGFQLAADNDQSENGEMGADRVSPYVRKAPYHFGWDWGPRLVTSGIWRPV
                     KLRAWSGARIESVKITTIEITSEMASVEAEVEVEVARAGRYWINVRQRDGVPRDAIPR
                     DAIPGLHLVKIPLTIHNPKTWNPNGLGEQYIYHFDIELTNNDKVVDETEVTAGLRRVR
                     LVQEPDPDGKGRSFYFEVNGKPVFAKGANYIPNDIFLNRVTPDRYEFIVKSAADAHMN
                     MIRVWGGGVYEDDLFYELCDQYGIMVWQDFMFACAMYPGNDEFLENVRLEAIDNVKRL
                     RNHPCLVLWCGNNEIEAAWGPYDENRGWGWKQRYNEEQREIIWQAYDTLFHKILPGVV
                     QQYDPGT"
     gene            complement(1608..2612)
                     /locus_tag="PKZ74_05170"
     CDS             complement(1608..2612)
                     /locus_tag="PKZ74_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM92183.1"
                     /translation="MSHGRFDNPVVASMAGFWDRFRKQELESHFTEKDRFDGKTVLVT
                     GANSGLGFAISTEIARRGGRVIMACRRQIPEAGEKVKAISGSENVEMRHLDLTRIDSI
                     HAFADGLVRDGIKLDVTVLNAGVTPPKARRTQSGLDEMFLVNYLSNVMLTHLLISSGT
                     INLNYDSAIFRPRIIFISSDSHQGSSYVDYDEFGRYFDYGVSKAIANYSYFKLVLNTY
                     ATELSKYINRDGIRAGIHVICPGPVNSNIVKEAPLLLRLILKGIFTIIFKSPAKACKP
                     VVYLAISPDFEGMTNEYLHMFRPKRMDPKVYLPEEGEKLWKRSLELWQSVDPMARIIP
                     "
     gene            complement(2700..3722)
                     /locus_tag="PKZ74_05175"
     CDS             complement(2700..3722)
                     /locus_tag="PKZ74_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HPM92184.1"
                     /translation="MIIRSKAPLRLGLAGGGTDVAPYSDLYGGATLNATINMYAYATI
                     IPKDDGKIVFKSIDKDKYFESPLTDSLEIDGNLNLLKGIYNRIVKDYSHAPLAFELST
                     YVDAPPGSGLGTSSTLVVAVLGAFAEWLRLPLGEYDLARLAYEIERVDLRMAGGKQDQ
                     YAATFGGVNYMEFYKDDKVIVNPLRIKSVYLNELSNNLVLYHTKTSRVSSAIIERQSK
                     NIQDNKLKFIEATHKLKEQAIMMKEAILKGRMDDIGPVLDYGWKFKKQIASGITNHLI
                     DDIYIAAMENGATGGKVSGAGGGGFMVFYCPNNSKYAVIEILKKFGGTSHPYEFTTSG
                     MTAWTI"
     gene            complement(3719..4990)
                     /locus_tag="PKZ74_05180"
     CDS             complement(3719..4990)
                     /locus_tag="PKZ74_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM92185.1"
                     /translation="MDKIVVFGPGPRFKGGIANYNTSLAKALDKLDVAEIYIISWTQQ
                     YPAIIPRDFIDRSSKSDLLEGTKIKVNYITNYNNPFSWNKTVKLIREINPKMVIFQWA
                     IAIQGLPMGYIAKKLKKIKGLEVVFDLHFVIQKEGSKIDKIFTRMGIRHADSYVVHAY
                     KTFDELKKLFPAVGFKLIDPGNRTEGMGKKVVKLFHPVYDMFKPDPDFDVEEEKRRLG
                     LKKNVFLFFGFIRKYKGLHDVIRAFAKIAGQRDDVSLLIVGESFWQTLDTTKLSTRVK
                     NAMFSAAKSVFLRKQDDERNYRPLDLIGELDLKDKVTVVNAFVPNEDVHRYFQVSDAI
                     MLYYLTATPSGVESIGYNFRMPILATRVGHFPETVKDGYNGYLAEPGDLDSMAEVMVK
                     AIEQPISRENVSETSREMSWLNYAKAILNER"
     gene            complement(5003..6061)
                     /locus_tag="PKZ74_05185"
     CDS             complement(5003..6061)
                     /locus_tag="PKZ74_05185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM92186.1"
                     /translation="MKIHLVSGGCGFVGRNMTRRLLKTTNDKVFIIDDLSIGTHPSTW
                     LENFSSRFYKDVEIIGKDERLYFWKGDFRNFLFKFRENPRFVQETYGLDFERFSDVFH
                     FAAIVGGRLKIDGDPMAVALDLSIDAEFFYWITRHKPERVLYPSSSAAYPTSLQTESD
                     AIALAEGDIDFKKNLGTPDMTYGWSKLTGEFLAQIAAKYYGVSIVCIRPFSGYGEDQD
                     LSYPVPAIAARAAKREDPFEVWGSGKQGRDFVHIDDILDLIFILMDNVHDGSGWNIGS
                     GKLTSFLELIDLFCDIAGYKPTIKPLLDKPVGVYSRYCKMDQVQARFGWEPKITLREG
                     MTRVYNAAVERLKKEGKL"
     gene            complement(6216..7175)
                     /locus_tag="PKZ74_05190"
     CDS             complement(6216..7175)
                     /locus_tag="PKZ74_05190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM92187.1"
                     /translation="MDISIIIPLLNEEESIPELHAWILRTLAPAGLTYEIIFVDDGST
                     DQSWKIIEDLSAGNPDVKGIRFRRNYGKSAALSSGFAAARGEVVITMDADLQDSPEEI
                     PELFRMITAEGFDLVSGWKKKRHDPITKTIPSRFFNWVTRKTSGIKLHDFNCGLKAYR
                     HDVIKSIEVYGEMHRYIPVIAKYAGFIRIGEKVVAHQSRKYGVTKFGIDRFIKGYLDL
                     LSITFVSKFGRRPMHFFGMIGTIMFVLGFLAAAWLGAQKIYNLQHGIKSILVTESPYF
                     FISLTLMIMGTILFVTGFLAEMLSRMSLERNRYLVSKTVNIEN"
     gene            complement(7185..7718)
                     /locus_tag="PKZ74_05195"
     CDS             complement(7185..7718)
                     /locus_tag="PKZ74_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPM92188.1"
                     /translation="MENKPNTLLICLGYGVIIALAGIVFSLILFVTGADETQSWMQNL
                     TYIITLAGILWAQYNYRNKYNNGFITYGEAFKIGLLTVLFLSVITSVYTFVFFKFIDP
                     GAMNEIMLKAEQSMLDQGRTDMEIEQGMKFMQMVNNVGWYTFFGFLGTFVIGIIISLI
                     TSAFIKKENTNFSQPNF"
     gene            7854..7952
                     /gene="ffs"
                     /locus_tag="PKZ74_05200"
     ncRNA           7854..7952
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKZ74_05200"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8070..8681
                     /locus_tag="PKZ74_05205"
     CDS             8070..8681
                     /locus_tag="PKZ74_05205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPM92189.1"
                     /translation="MLIKIYDKNPSEKAIRQAAKVLEDGGLVIFPTDTIYAIGCSIYS
                     QRALERMARIKGVNPKNANFSFLFYDLSQLSEYTRPISNEVYKLMKRTLPGPYTFILE
                     ANSNVPKILGIKKKTVGIRIPDNGILLEIMRELGKPVMSTSVHDDDEVIEYTTDPELI
                     HERYEDLVDLVIDGGHGDNIPSTVYDCTGEEIVVVREGKGDIE"
     gene            8731..8803
                     /locus_tag="PKZ74_05210"
     tRNA            8731..8803
                     /locus_tag="PKZ74_05210"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8764..8766,aa:Lys,seq:ttt)
     gene            complement(8848..10086)
                     /locus_tag="PKZ74_05215"
     CDS             complement(8848..10086)
                     /locus_tag="PKZ74_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM92190.1"
                     /translation="MKTFMLRFRTVLLLFLFSMIFVGANSQSSYLLVRGILKDSKTGE
                     AIRFAAITVPGTDIGTVSNSDGEFTLKVSNVLKAEYFEVSHISYATSRFKISEATGEV
                     RTYLIELQPVQLKEVPVIDADARKIVEMAFRNIRQNYSTEPNMMTGFYREYIMQRRDY
                     LSISEAVVDIYNAPYQGFQNDQVKIFKGRKSSDVKNADTLIVRLQGGPYVLLLLDIVK
                     NTDLSIGLDDLDNYKYEISRYVQIDDKLHWVVDFVPAVNRENALYKGKLYISQENYAI
                     SRAEFSLDLSDEEKAASSFIKKKPSGMVFAPVSTGYIVTFKEQESRYYLNYVRVDLKF
                     RCDWRKKLFRNTYTLMSEMAITGRNSGNIVKFENEDQFRSSMIFSEKVTDFSDPDFWG
                     EHNIIEPEQSIENAIRKLMK"
     gene            complement(10249..>11963)
                     /locus_tag="PKZ74_05220"
     CDS             complement(10249..>11963)
                     /locus_tag="PKZ74_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPM92191.1"
                     /translation="FCLSGTTGNGMFKIYTAKRYNLILNGVNLTNPDGPAINVQTGKN
                     TTVILAEGSTNTLADGTTYAEPPAGEDQDGAFFSEATLKFTGTGTLIINGHGTDKHGL
                     CSDDEIEVNEGTININCASGDGINGKEGVIISGGVIHITVNGDQSKGINCNSTLTLSG
                     GDITVHNTGDAVLVQAGSGYDPSYCTAVKADGNIEINGATVTIVASGKAGRGISADTD
                     IIISSGSVQVTSTGNGAVYTDSSGILDAYVATCFDSEGDIMITGGSVITSSSGSGGKG
                     INSGSELIIGTPESSPIIQITTTGTKVHISGGGQNSKDAEAKAMKIDTAVVINNGEIT
                     ISSSDDAIKSENSITVNGGDIQVNNSYEGFEAPFITINDGNLHLYASNDGLNATFGSG
                     GEGDDNSLLTINGGWVMLSVSNGDGLDSNGDIHITGGTTIVHGPQNAPEVGMDYNGTC
                     NMDGGFLVISGTNSFMTQAPDNSSDQHCVKVMMNQGLSNSTLFHIQDASGNNLLTFQP
                     ARTYYSIVFSSSDLQTGQSYSIYTGGSCTGINVDGLYSGGVYSGGTFRKSFTVNSIIT
                     NVNF"
BASE COUNT         3110 a         2992 c         2553 g         3308 t
ORIGIN      
        1 gttccgggat catattgctg cactacaccg ggcaggattt tgtgaaacaa cgtatcatag
       61 gcctgccaga tgatctcccg ctgttcctca ttataccttt gtttccagcc ccatcccctg
      121 ttttcatcgt acgggcccca tgccgcttcg atctcgttat taccgcacca taacaccagg
      181 cagggatggt ttcgtaaccg tttcacatta tcaattgctt cgagcctgac attttcgaga
      241 aattcatcat ttcccgggta catggcgcag gcaaacataa aatcctgcca gaccatgata
      301 ccgtattggt cgcataattc gtaaaacagg tcatcttcat aaacaccacc gccccagaca
      361 cgtatcatat tcatatgggc atctgctgcc gatttgacga tgaactcata ccggtcgggt
      421 gttacccggt ttaaaaatat atcgttgggg atatagttag ctcctttggc aaaaaccggt
      481 tttccgttga cttcaaaata aaaactccgg cctttgccgt ccgggtcggg ttcctgtacc
      541 agcctgacac gacggagacc ggccgtaact tctgtttcat caacgacttt atcattattg
      601 gtcaattcaa tgtcaaagtg ataaatatac tgctctccga gcccgtttgg attccaggtc
      661 ttcgggttat ggatggtcaa tgggattttc accagatgaa ggcccgggat ggcgtccctc
      721 gggatggcgt cccgagggac gccatccctt tgtctgacat ttatccaata cctccccgcc
      781 cttgccacct ctacctctac ctccgcttca acagatgcca tctcagaggt tatttcaatg
      841 gtcgttatct tcacactttc tatccgcgca ccgctccagg ccctcaactt taccggcctc
      901 caaatccccg aagtcaccag cctgggtccc cagtcccagc caaaatgata tggcgctttt
      961 ctcacatacg gactgacccg gtcagcaccc atctcgccgt tttccgactg atcgttgtcg
     1021 gcagccagtt gaaaaccata cgcacttaac gcctccaatc ctttcctggt tggcgattcc
     1081 agcaaaacga caagctcatt atcccccgcc ctgatcttgc ccttcacatc tgcggtccac
     1141 cgcctgaaca tgttgtcggc ctccaggatc aaagatccgt taagaaaaac acgggcatat
     1201 gtatccagcc cttcgaaatc aagctcaacc cggtcattat taaggacatc cttatccaca
     1261 taaaaacttg tcctgtattc ccagtctttt ttgtcaatcc attgcaactg cttttcgttc
     1321 aaccgataat aagggtcttc aatgatgttg ttaagtagaa gatcggtatg tacggtcccc
     1381 ggaacaacgg ctttatacca cgggccggtt tccatctgac ggaaactcca gttcgacttt
     1441 aatgccactt ccagcggctg aacttgctcc ggttccggat tacaaccagc cagcccagta
     1501 acaatcgcaa gaatagaaaa gacacaatta tacaaacgat tcattctcag ttctctattt
     1561 atattaggct tacaaacggg tttaataaaa acagttgcaa taattgctta cgggattatc
     1621 cttgccatgg gatcaaccga ctgccaaagt tccagcgacc ttttccataa tttctcgcct
     1681 tcttccggaa ggtatacttt aggatccatc ctttttggtc tgaacatgtg aagatactcg
     1741 ttcgtcatgc cttcaaaatc cggggaaata gccaggtaaa caacaggttt gcaggctttt
     1801 gcgggagatt taaagatgat ggtgaatatc cccttcaaaa taagtctcaa taataaaggt
     1861 gcctccttga caatgttgga attgaccgga ccgggacaaa tcacatggat ccctgccctg
     1921 ataccatcgc ggtttatata cttcgacaat tcggttgcat aagtgttaag cactaactta
     1981 aaatagctgt aatttgcaat cgcttttgac acgccatagt cgaaataccg gccaaattca
     2041 tcataatcaa cataagatga accctggtgg gagtcggagg aaataaagat gatcctgggc
     2101 ctgaaaatag cggaatcata attcagattg atagttccgg agctaatcag caaatgggtc
     2161 agcattacat tagagagata gttgaccagg aacatttcat caagtcccga ttgggtcctg
     2221 cgtgctttcg gcggagtgac cccggcgttt aagacagtga catccagttt aataccatcc
     2281 ctgacaagtc cgtcggcaaa cgcatggatg ctgtcgatac gggtcaggtc aaggtgccgc
     2341 atttcaacat tttccgaccc gctgatcgcc tttacctttt caccggcctc ggggatctgc
     2401 cggcgacagg ccatgatcac acgcccgcct ctccgggcaa tctcggtact aatggcaaaa
     2461 cccagccccg aattcgcccc ggtaaccagc actgttttcc cgtcaaaacg atccttttca
     2521 gtaaaatgtg actcgagctc ctgctttctg aacctgtccc aaaaaccggc catcgaagca
     2581 actaccggat tatcaaatcg tccgtgcgac acaaaagaga attttaaatt tttaattctt
     2641 gatttttaat tgaccttgaa ctttccactt tgaactttga actttgaact ttgaactgat
     2701 cagatagtcc atgccgtcat gccactggtg gtaaattcat acgggtggct ggttcctccg
     2761 aattttttaa gaatctcaat gacagcgtac tttgaattat tgggacagta aaagaccatg
     2821 aaaccgccac cacctgctcc cgagacttta cctcctgtcg caccattctc catggctgct
     2881 atataaatat cgtcaatcag gtgattggtg atgcctgatg caatctgctt tttaaatttc
     2941 cagccgtaat ccagcacagg gccgatatca tccatcctgc ccttgaggat agcctctttc
     3001 atcatgatgg cctgctcctt cagcttatga gttgcttcaa tgaatttcag cttgttatcc
     3061 tggatatttt tcgattggcg ttcgataatg gctgatgaga cccggctcgt tttcgtatga
     3121 taaagaacta aattattgga taattcatta agatagacac ttttaattct cagaggattg
     3181 acaataactt tatcatcctt atagaattcc atataattca ccccgccgaa tgtcgctgca
     3241 tattggtcct gtttgccgcc agccatgcgc aaatcaaccc gttcaatttc atatgccagg
     3301 cgggccaggt cgtattctcc caacggcaat ctgagccatt ctgcaaaagc ccctaaaaca
     3361 gctaccacaa gcgtcgaaga agttccaagt cctgatccgg gaggagcatc tacataagtt
     3421 gaaagttcaa aggccaaagg tgcatgcgaa taatccttta cgatccggtt gtatatacct
     3481 ttcagcagat tgaggtttcc atcaatttcc aggctgtcgg tcaggggcga ttcaaagtat
     3541 ttatccttgt cgatggactt aaagacgatc tttccgtcgt cttttggaat gatcgtggca
     3601 taagcgtaca tattgatagt ggcattgagt gtggccccgc catacagatc cgaataagga
     3661 gcgacatcag tgcctccacc ggccaggccc agcctgagag gtgctttgct acggattatc
     3721 atcgctcgtt aaggattgct tttgcataat tcaaccagct catttcacgg gatgtctccg
     3781 acacattctc cctggagatc ggttgttcta tcgctttgac catcacctcc gccatcgaat
     3841 ccaggtcacc cggttcagcc aggtaaccgt tatagccatc cttgaccgtt tcagggaaat
     3901 gcccgacccg ggtagccagg atcggcatcc ggaaattata gccgatcgat tcaacgccgg
     3961 acggggttgc tgtgagatag taaagcataa tggcatcact cacctggaaa taccggtgaa
     4021 catcctcgtt gggaacaaag gcattaacca cggtcacttt atcctttaga tccagttcac
     4081 cgatcaaatc taatggcctg tagttgcgtt cgtcatcctg tttacgcaga aaaacggatt
     4141 tggctgctga aaacattgcg ttcttgaccc gggtggaaag ttttgttgta tccagtgttt
     4201 gccagaacga ttcgccgaca atgagcaggg aaacatcatc cctttgcccg gctattttgg
     4261 caaaagcgcg gatgacatca tgcaagcctt tgtatttcct gataaatcca aaaaagagga
     4321 aaacattttt tttcaatcca agccgccgct tttcctcctc cacatcaaaa tcaggatccg
     4381 gtttgaacat gtcataaacc gggtggaaaa gcttaaccac ctttttaccc attccttccg
     4441 ttcggttccc cgggtctatc agcttaaagc cgaccgccgg gaacagcttt ttcaactcat
     4501 cgaaagtctt ataggcatgc acgacatagg aatccgcatg ccgtattccc atccgggtaa
     4561 atattttatc gattttgctc ccctctttct ggatgacaaa atgcaagtca aagaccacct
     4621 ccagcccctt tatcttcttg agttttttag cgatataacc catgggaagg ccctggatgg
     4681 ctatggccca ctggaagatg accatctttg gattgatctc ccggattagc tttaccgttt
     4741 tattccagct gaatgggttg ttataattgg tgatgtaatt gactttgatc ttcgtccctt
     4801 ccagcaagtc cgatttactg ctcctgtcga tgaaatcccg cgggatgatg gcaggatatt
     4861 gctgggtcca ggaaatgatg tatatttcgg caacatccag tttatcgagg gcttttgcca
     4921 gggaagtgtt ataattggca atgcctcctt taaaccgggg gccggggccg aatacgacga
     4981 ttttatccat ttccggcctg ggtcagagtt ttccttcttt tttcagtctt tcgacggcag
     5041 cattgtaaac acgggtcatc ccttcccgca gggtgatctt cggttcccat ccgaatcggg
     5101 cctgtacctg gtccatttta caatagcggg aatatacgcc gaccggtttg tcgagcaaag
     5161 gttttatggt tggtttataa ccggcgatat cacagaacag atcgatcagt tcgaggaagg
     5221 aggtcagttt gccggagccg atattccagc cggatccgtc atggacatta tccatcagga
     5281 tgaaaataag gtcgaggatg tcgtcgatgt ggacgaaatc cctcccctgt tttccgcttc
     5341 cccacacttc gaaaggatct tcgcgtttgg cagcgcgggc agcgatagcc ggaaccgggt
     5401 acgaaaggtc ctggtcctca ccatagcccg aaaagggcct gatgcaaaca atagagacgc
     5461 cgtagtattt agcagcgatc tgggcaagga attcccctgt cagcttcgac cagccatagg
     5521 tcatatccgg cgtgcccagg tttttcttga agtcgatatc cccttccgcc agggcaatgg
     5581 catcgctctc ggtctgcaaa ctggtcgggt atgcggcgct ggagctgggg tagagaactc
     5641 tttcgggctt atggcgcgtg atccagtaga aaaattcagc atcaatcgac aggtcaaggg
     5701 caacggccat gggatctcca tctatcttca acctgccgcc gacaatagcg gcaaagtgga
     5761 acacatccga aaaacgttcg aaatcgagcc cgtaggtctc ctgaacaaac cgtggatttt
     5821 ccctgaattt aaagagaaaa ttgcgaaagt cccccttcca gaagtacagt ctttcatctt
     5881 tcccgatgat ttcgacatct ttataaaacc tggaagaaaa attttcaagc catgtcgatg
     5941 gatgtgtccc tatcgaaagg tcatcaataa taaaaacctt atcgtttgta gtcttcaaca
     6001 accggcgggt catattcctt ccgacaaaac cacaaccacc tgatactaaa tggattttca
     6061 tgagttctat tgttcgattg ttcgattgtt cgattgttct attgttctat tgttctattg
     6121 ttctattgtt ccgggtttca ggttccaaat tatcaaattt ccaaattatc aaatttccaa
     6181 attatcaaat ttccaaatta tcaaatttcc gaatttcaat tttcaatatt caccgtcttc
     6241 gacacgagat acctgttcct ttccagcgac attctcgaca acatctctgc caggaaacct
     6301 gtaacaaaga gtatggtccc catgatcatc aaagtcaatg aaatgaaaaa gtacgggctt
     6361 tctgtgacca ggatactttt aatgccatgc tgcaggttat agatcttttg tgcgcccaac
     6421 caggctgctg ccagaaaacc aagcacaaac atgatcgtgc ctatcatacc gaaaaaatgc
     6481 atcgggcggc gcccgaattt ggaaacaaac gtaatcgata acaagtcgag gtaacctttg
     6541 ataaagcggt cgatgccgaa cttggtcacg ccgtattttc tgctctggtg ggccactacc
     6601 ttttcgccga tccgaataaa gcctgcgtat ttggcaataa ccgggatata tcggtgcatc
     6661 tcgccataca cctcaatgct tttaattaca tcatgcctgt aggcttttaa tccgcaattg
     6721 aaatcatgca gtttaatccc cgatgttttc cttgtcaccc aattgaagaa ccgtgaaggg
     6781 atagtctttg ttattggatc atgccgcttc ttcttccagc ccgaaaccaa atcaaatcct
     6841 tccgctgtta tcatccggaa cagttccggg atttcttccg ggctatcctg gagatcggca
     6901 tccatggtaa tcaccacctc tccccgggcg gcagcaaaac cactgctcag ggcagccgat
     6961 ttgccatagt ttcgccgaaa gcggattcct ttaacatctg gatttccggc actcaggtct
     7021 tcaatgattt tccacgattg atcggtgctg ccgtcatcca caaaaatgat ctcataagtt
     7081 aatccggcag gagcaagggt tctgagtatc cacgcatgaa gttccggaat cgattcttcc
     7141 tcgttaagaa gaggtatgat aatggaaata tccatcgtga attttcagaa attaggctgg
     7201 ctgaaattgg tattttcttt ctttatgaag gcggatgtga tcaggctgat aatgataccg
     7261 atgacaaatg tacccagaaa tccgaagaaa gtgtaccaac ccacattgtt aaccatttgc
     7321 atgaacttca tcccctgttc gatctccata tcggtccttc cctggtcaag catcgactgt
     7381 tcggccttga gcatgatctc gttcattgct cccggatcga tgaatttaaa aaacacaaaa
     7441 gtataaacac tggtgatcac cgacaggaag agtaccgtta gcagccctat tttaaaggct
     7501 tcgccatagg tgataaagcc attgttatat ttgttcctgt aattgtactg tgcccacagg
     7561 atacccgcca gggtgatgat gtacgtaagg ttttgcatcc agctctgtgt ttcatccgcg
     7621 ccagtcacaa acaaaataag gctgaagacg ataccggcca gggcaataat cacaccatac
     7681 ccgaggcaga tcaaaagcgt gtttggttta ttttccataa ttatcaggtt ttgttacctg
     7741 caaagctagg aaatttagtt caaagtccaa agatcaaaga tcaaagttac ctgaagctcc
     7801 aactttgcaa tttgatcttt gaactttgaa ctatttgttg tacttttgcc gctgggtaag
     7861 tctcatgcga ccagctcctg ctgaactccc ccaggtccgg aaggaagcaa gggtaagcgg
     7921 ttgagcggtg cgacatgagg ggcttaccct tttttattgt ccattgtcca ttgtccattg
     7981 tccattgtac cttatttatt acaatctatt tacttttcat tattcactat tcgtttttca
     8041 ctattcctta ttattagtaa atttgtcaga tgctgatcaa gatatacgat aagaacccca
     8101 gtgagaaagc gatccggcag gcggcaaagg tgctggaaga cggcggcctg gtcatctttc
     8161 cgaccgacac gatctacgcc atcggatgca gtatatacag ccagagagcg cttgaacgga
     8221 tggcccgaat caaaggggtc aacccgaaaa atgcgaactt ctcctttctt ttttatgacc
     8281 tgagccaact atcggagtac acccgtccga tcagcaatga ggtctacaaa ctgatgaaaa
     8341 ggacattgcc aggcccgtat acgtttatcc tcgaggccaa cagcaatgtg ccgaaaatat
     8401 tgggcatcaa gaaaaagacg gtcggtatcc gaatccccga taatggcata ttgctggaga
     8461 tcatgcggga actgggcaaa ccggtcatgt ccacatcagt ccacgacgac gacgaagtga
     8521 ttgaatatac caccgatccg gaactgatcc acgaacggta cgaagatctg gtggacctgg
     8581 tcatcgacgg ggggcacggc gacaatatcc ccagtaccgt ttacgactgc accggtgagg
     8641 aaattgtagt ggtcagggaa ggaaaaggcg acatcgaata aaattttaaa ctttgaacta
     8701 aaaattttct atctttgcgc cctcatttga gattctgtag ctcagccggt agagcaacgg
     8761 ccttttaagt cgtgggtccc gggttcgaat cccggcagga tcaccccaac attgcgcatt
     8821 gcacattttg cattgcgcat ttttcattta cttcattaac ttcctgattg cattctcgat
     8881 cgattgctcc ggttcgataa tgttatgctc tccccaaaaa tcaggatcgg aaaagtctgt
     8941 tactttttcg gagaatatca tcgatgacct gaactggtct tcattctcaa attttacgat
     9001 attgcccgaa ttccgaccgg taatggccat ttccgacatt aaggtgtacg tatttctgaa
     9061 aagcttcttt ctccagtcgc accgaaattt caggtcaacc ctgacataat taaggtaata
     9121 cctgctctcc tgctctttaa acgtaacgat atatcctgtt gacaccggtg caaagaccat
     9181 cccggaaggt tttttcttaa taaaagagga tgctgccttt tcctcatccg aaaggtcaag
     9241 gctgaattcg gcacgcgaaa tggcataatt ttcctgcgaa atgtaaagct tacctttata
     9301 taatgcattt tctctgttga cagcaggaac gaaatcaacc acccaatgca gtttgtcatc
     9361 gatttgaaca tacctggaga tctcatattt gtaattatcc agatcatcca gtccaatact
     9421 gagatcggta tttttcacaa tatccagaag cagcagcaca taggggccac cttgaaggcg
     9481 gactatcagc gtatcggcat tcttcacatc ggaacttttc cggcctttga agatcttcac
     9541 ctggtcgttc tgaaaaccct ggtaaggagc attatatata tcgactaccg cctcggaaat
     9601 cgaaagatag tccctccgct gcatgatata ttccctgtaa aagcccgtca tcatgtttgg
     9661 ctcggtgctg tagttctgcc ggatgttccg gaaagccatc tccacgatct tcctggcatc
     9721 ggcatcgatt acaggaacct cttttaactg aaccggttgt aattctatta agtaggtcct
     9781 gacctctccc gttgcctcag aaattttgaa ccgcgatgta gcatagctga tatgcgaaac
     9841 ttcaaaatat tcagctttta aaacattgct gacctttaaa gtgaactcac catcggaatt
     9901 cgaaacggtt ccgatatctg tcccgggaac ggtaatagcc gcaaagcgta tggcttcgcc
     9961 ggtcttgctg tctttcagga tacctcttac cagaagatag gaactctggc tgtttgcccc
    10021 aacaaatatc atactgaata agaaaaggag aagaacagtc ctgaatctaa gcatgaaagt
    10081 tttcatttcg agaagattag aagttatcga ataatcgcaa ggattttatg cttaatacca
    10141 ttccaggaag tacaccggca caacctgaaa tgagtggatg aaattaatga tttttaagtg
    10201 aatcccgtga aaactggcta aaataatatc aacataaacg ggtgaagatc agaaattcac
    10261 attggttatg atactattca cagtaaaaga cttacggaat gttcccccgg aataaacgcc
    10321 tccgctgtac aaaccatcca cattgatccc cgtgcatgat cctccggtat aaatcgagta
    10381 gctttgccct gtctgaagat ccgatgacga aaaaacgata gagtaatagg ttcgagcagg
    10441 ttgaaacgtc agtaaattgt ttcctgaagc atcctgaatg tggaataaag ttgaatttga
    10501 gagtccctgg ttcatcataa cttttacgca atgctgatcg gaagaattat ccggggcctg
    10561 ggtcataaag gagttagttc cggaaatgac caggaaaccg ccgtccatat tgcaagtacc
    10621 attgtaatcc atacccactt ccggagcgtt ctggggcccg tgaactatgg tcgtgccccc
    10681 ggtgatatgg atatcgccat tgctgtcgag tccatcacca ttggaaacac taagcattac
    10741 ccagcctccg ttgatggtca gaaggctgtt atcgtctcct tcgcctcccg acccgaaagt
    10801 tgcattcaaa ccgtcgttgg aagcatacag gtgcaggttg ccatcattaa tggtaataaa
    10861 aggagcttca aatccttcat aggaattatt gacctggata tctcccccat tgaccgttat
    10921 gctgttttcc gatttgatcg catcatcgga agaagaaatt gttatttccc cgttgttgat
    10981 cactacagct gtatcgatct tcatcgcttt tgcctctgca tcctttgaat tctggccgcc
    11041 tcccgaaatg tgcactttcg tacccgttgt tgtaatctgg attatcgggg aagattccgg
    11101 tgtaccgatg atcagttcgc ttcctgagtt gatccctttt ccacctgagc ccgaactcga
    11161 ggtaataacc gagccgcctg tgatcatgat atcgccttca gagtcaaaac aggtcgccac
    11221 ataggcatcc aggatgcctg aggagtctgt ataaacagcc ccattcccgg tactggtcac
    11281 ctgcacactt cctgatgaaa taatgatatc tgtatccgca gatattcccc ggccggcttt
    11341 tcctgaggcg acaatcgtaa ctgttgctcc gttaatttca atgttgccat cggctttaac
    11401 cgccgtgcaa tacgacggat cataccccga cccggcttgt accagcacag catcgccggt
    11461 gttatgaacg gtgatgtctc cgccgctaag cgtaagggtg gaattacaat taattccctt
    11521 cgactggtca ccgttaacgg taatatggat tacccccccg gaaatgataa ctccttcttt
    11581 accattgatg ccatcccctg atgcacagtt gatattaatg gttccttcat ttacctctat
    11641 ttcatcatca ctgcaaaggc catgtttatc ggtaccatgt ccgttgatga tcagtgtacc
    11701 tgttcctgta aatttaagcg tggcttcgct gaagaaagcg ccatcctggt cttctccggc
    11761 cggaggctca gcatacgtcg tcccatcggc cagggtattg gtagagccct ctgcgaggat
    11821 caccgtggta ttcttacctg tctgtacatt gattgccggt ccatccggat tggttagatt
    11881 tacgccgttg agaatcaggt tataccgttt tgcggtataa atcttgaaca tcccgttgcc
    11941 tgttgtcccc gagaggcaga agt
//
LOCUS       DAOPNH010000069        11959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5618_length_11959_cov_7.322917, whole genome shotgun sequence.
ACCESSION   DAOPNH010000069 DAOPNH010000000
VERSION     DAOPNH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_11959_cov_7.322917"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1790)
                     /gene="cysS"
                     /locus_tag="PKZ74_05225"
     CDS             complement(246..1790)
                     /gene="cysS"
                     /locus_tag="PKZ74_05225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPM92192.1"
                     /translation="MQQLYIYNSLSRKKELFEPLVPGHAGMYVCGPTVYGDPHLGHAR
                     PAVTFDLVFRYLKHIGFKVRYVRNITDVGHLEGDADAGDDKIGKKARLEQLEPMEVVQ
                     YYTNRYHELMARLNLLNPSIEPHASGHIIEQIAMVQKILDAGFAYERNGSVYFDIEKY
                     NQKHPYGILSGRKVDELLAFTRELDGQEEKKSSFDFALWKKATPDHIMRWPSPWGEGY
                     PGWHLECSAMGCKYLGETFDIHGGGMDLLFPHHESEIAQSMAATGKMPVRYWMHNNMI
                     TINGQKMGKSLGNAVSLEDFFNGNHSLLEKAYSPMTIRFFILQAHYRGTLDFSNEALQ
                     ASEKGLRKLLAALAIVQSSKFKVQNLQKLKFGNVYYEDLELWNPEPGTVTGPIAQIIA
                     NSYSAMNDDFNSPIVIAQLFEAARIINLVKEGKEKLSDNDMAALAFAYRTFMVDILGL
                     VPEEQSGQKDEIISGLMDTIIGIRADARAKKDFATSDQIRDKLLKVNIQIKDGKEGAT
                     WEIKED"
     gene            complement(1942..2769)
                     /locus_tag="PKZ74_05230"
     CDS             complement(1942..2769)
                     /locus_tag="PKZ74_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92193.1"
                     /translation="MIKSIKYYVLINGFAIILAILMVGMVGGCAEKRDGIPAQMTFSR
                     FYYEKSGRQAAISNVFEMKEGENVVAVVDPGVSLMDPGRDRLFHIDWIGPGNRSVYLK
                     RIDLPAGDSTGKLVSAVSLDPLKRDAGVYSVRVYYFRDLIAEKQFEMVPEGTANHIEA
                     DIIFYKSLDKETGEMNGIDTVFEIRKKGILRARVNLRNMGIYEDDELPFRMEWYGPDG
                     KSFYDKKMMVNPADTVVTLNGSVSITPDKRQPGDYELRVLLFDDIIVKRRFFLRSED"
     gene            complement(2879..3997)
                     /locus_tag="PKZ74_05235"
     CDS             complement(2879..3997)
                     /locus_tag="PKZ74_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPM92194.1"
                     /translation="MALINSAPGPSRFDYIDQVRGLVVVLMLLDHCSYYFNSVWDQID
                     PLDPLFDSWPQFALRYASYLCAPGFLILAGAMVWWSYTKQIEKGIERKKIIRQIMIRG
                     LFLVVLQVTWVNSSWGGFSEFKPGHLGIIACIGISMILLALVIRYHWMVRAALGLLIL
                     ALHPLLLRIPYDPATGWQEALMQTFIDAGEFNKYPVIPWFALALLGSAMASGWLQAWK
                     SDRAKISWSFGIGAAAILLALLVRMGRGYGNIFPFSDPGSYSFFFDQKYPPSLFLNLW
                     FFGWVLVITGIFLAINKISPKILYFFAIPGRVALFFYGMHIAIMGIFIKRIDFLYRDG
                     GVTETLIAFAVMMVVMIPLCLWFYNVKKRSRNWVVRMI"
     gene            complement(3984..4994)
                     /locus_tag="PKZ74_05240"
     CDS             complement(3984..4994)
                     /locus_tag="PKZ74_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92195.1"
                     /translation="MNKNLLLFVSGVILLAGCGKPKDPESLTPSDGGYKIVSRIASSG
                     FSNDVVKKDNLLYIAQGEGGLLIYDITNPAVPSFISLTTDKLRGYAGKIAIRDSAVYL
                     AANTFGINVVNVSQPDTPRVAITNLGVKPARNFHIMGHYLFTAISEQGVGIAEISYPT
                     EPDVRGQFATIGYAYGLATSSDSNYLFVAGGEMGLTIYNISDFQEGYGIYPKAGWCDT
                     PGDAEAIVLDENRSLAFMACGTSGLQILDYSDTAGVFVAGVYDSAGYAKDLYLDDNKI
                     YMSAQLGGMQIIDVTNPAAPKLIGAVKTKYALGIDADDQYIYLADEDEGVLIISIPYG
                     LD"
     gene            complement(5007..6146)
                     /locus_tag="PKZ74_05245"
     CDS             complement(5007..6146)
                     /locus_tag="PKZ74_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92196.1"
                     /translation="MRIFIQKYYRCCLPTYFLIILFLVFGMQTAAAQKKSSKKEKHFE
                     AEIKFGSMYDDNILKYSGKYIDRFINRQDSGRFHIKTYDDIVFITAADLSATYRIFKD
                     LKTRINGSIYSNMYATNGVKNWYYFTLGLTQNVTKKASFRVSYSHIPRFYVRHFRDAD
                     RVEIYGYTPETFVPFSFAKDNYGFWFQNTFLKSTRVRLSFDLARYFHNKHYTEYDCNS
                     YIFGISVSQPLHKKVKIEAAYEFEHSDAKGYDEPGETRETADDADATYNEYAFALGLN
                     WELPKIRKKTHEIDLNATYQNRVYFSEHFLEEDPEHTGRVDDVVLVTANYNLQVSKSF
                     TLGAFYKFFMRNSNSSSEINSEYLSEEKDYRQDQVGLQVVYNLKF"
     gene            complement(6159..7067)
                     /locus_tag="PKZ74_05250"
     CDS             complement(6159..7067)
                     /locus_tag="PKZ74_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92197.1"
                     /translation="MMIKKFGVFGFLLLLTGSICAQTESKTIKPSGFVKKITTVSAGK
                     NRNYYALSYADAAMVTVQGPGVLKVHTRAQFRPGDQVQARYTVIYTVDGGTQKSVSVS
                     GIERSRNATYTDGTLGVPGELKTFEIELSRGFHTVEFRLKDITANVAARYVFTPAKLK
                     KQDWMAFSPLRPSEPVDLISRETTTPYYRFTAEKPLRVEIIGPTELRVLIRTEHHFGM
                     KGRINYRVQVRENGSVINTYQLNSKVSDVAVYKDIDNLIPGTACEFVILIPDGKHLYE
                     ICPLDQDKNSLLGRLLIPGKDVGLEE"
     gene            complement(7130..8287)
                     /gene="pgsW"
                     /locus_tag="PKZ74_05255"
     CDS             complement(7130..8287)
                     /gene="pgsW"
                     /locus_tag="PKZ74_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPM92198.1"
                     /translation="MFKFKATSNLVLGLLFLLALLAFIAVENGKKDVKQDYYNEKLEA
                     ARLSQAAAGHIKQYRLQKGVFLDDVNDPNQTALIGQEYTMITTDQGNVESKLSATNPN
                     FAAVIVQLLKEAGLEEGDPVAIAFSGSFPGMNISVMAAIETLKLQPTVITSVGASNFG
                     ANDPYFTWLDIETLLNDAGIFHYKSVAASIGGGNDIGRGLSPEGRNLIISAIERNRVE
                     LINEKHLENSIARRMEIYEQQGKKNAARAFINVGGGIASLGNTVNGQLIPSGLTERLP
                     MSNYPLHGVIIQMGRKGIPIIHLLNIDQLAAKYGLPANPVPMPEPGEGGIFIQKKYDL
                     VVTTIALAILVTSIIFIYLAERKRHQLGTDVVPVVPGQRKQQAEDEMIEEI"
     gene            complement(8296..8748)
                     /gene="pgsC"
                     /locus_tag="PKZ74_05260"
     CDS             complement(8296..8748)
                     /gene="pgsC"
                     /locus_tag="PKZ74_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HPM92199.1"
                     /translation="MIELAITLGLVLSLLSYEVFGLAAGGIVVPGYIALQLSHPDRLI
                     GILVVSLVTYLIIKVLGNYTFLYGRRQMVLSLLIGCLLAIFTRHFLTFNLGSSTLELE
                     AVGWVIPGLIAHWFAKQGIFKTISVLFVSSVLVRLVLILIFAGALLPK"
     gene            complement(8752..9930)
                     /gene="pgsB"
                     /locus_tag="PKZ74_05265"
     CDS             complement(8752..9930)
                     /gene="pgsB"
                     /locus_tag="PKZ74_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPM92200.1"
                     /translation="MHSFYILLALVLMAVIFGIIEYFLHQKRISSIPIRIHVNGTRGK
                     SSVTRLIGAGLREGGITNITKVTGTFPRLILEDGTETYIHRKASANIIEQLSIVRFAA
                     SRKVKALVMECMALQPQYQTITEHRMIHSNVGVMTNIRMDHTDVMGHTLGEIAETLGR
                     TIPAKEHLFTSENVIPDLLKKMADKRNTVTHFVDPEIVSHEEMQGFRYIEHRENVSLA
                     LAVCLHAGVDRNTALQGMYKAVPDAGALMVFRVEAFRKNLVFYNAFAANDPDSTFLVW
                     RKISDETGMEGKRIVLLNTRQDRLYRAEQLAGMVAYRLEKEVDYLVLIGQSTDVVESM
                     AIRYGIPARKVVTVGWTTSENVFDRLLALTDHTATIVAIGNMGGMGSGLVEFFEHRSI
                     "
     gene            complement(9943..>11959)
                     /locus_tag="PKZ74_05270"
     CDS             complement(9943..>11959)
                     /locus_tag="PKZ74_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPM92201.1"
                     /translation="PSIHIWRDVPLAVTVDYPQTILVGQDQVEITVNFTASGQPAANA
                     QVCLTGDEIFATGYCDSEGKVYMDIVPETPDTLKLTVRGKNVYPFQADIIVIQPEELV
                     EPDGGPALDDLDGNLDGLIDPNENGTATFTLKNWGSQTANNVMATLTSGMPDYMQVLT
                     TVPVNYGNLASGAKITGNPFMLRVAENCPVGQLIPVQLHVTSTENSWDYSYDIVINGC
                     NLLDEGFYVNDQGAAEPNYRMDPGETVKLFIAVHNAGNDLAKDVVGILTSDDPFIDIE
                     DGAGAFGTIAINETAINFYNYFVVKIDPSCPADYFAQFNLQLTTSNGNYPYQKNLTLN
                     LPVSHPVPSDYTGPDAYGYYAYSSDDIFDQAPGYTWIEIDQAGTPINISGIGDYTETV
                     SLPFTFKYYGVEYAQLRISTDGWIAFGNGNQTAPVNTTLPNIDNINCMVAGFWDDLYD
                     AETVEGNIYYYNDFSNHRVIIEWDSIARNDFGSEPNVEIFQAVLYDPDFYPTLTGDGE
                     IVFNYKQVRAPESSTIGIENQGQNIGLLYLFNDAYNPGSNILKNEFSIKFTTEPPEAS
                     IITGIDDRKEGSMETSDHLSCFPNPFVESTRISYTIDNPAAVSVKILDVEGRTVRILK
                     SARQNAGTYEILWDGCNDAGLPVSAGVYFCQIKSENFRETVKLFLLR"
BASE COUNT         2915 a         3088 c         2899 g         3057 t
ORIGIN      
        1 caggtacaga tcaatgtaga aagttatcct ccgggggtgt atatcgcggt tctgagagat
       61 gggggcggtt ttttgggaag tggtaagttt gtggtggcga gataagacca gttggacagt
      121 tgacagttgg acagttgggc agcatgtcct gagtccgcca aaggcggacg aaggatcgca
      181 gttggacaat atcattacgc gatttggcat tgcgcattcg gcattgtcga aaaatgcgga
      241 atcgtctaat cttccttaat ctcccacgtc gccccttcct tcccgtcctt aatctgtata
      301 ttgaccttta gcaacttatc cctgatctgg tcggaggtgg cgaaatcctt cttcgccctg
      361 gcatccgcgc gaatgccgat aatggtgtcc atcagcccgc tgatgatctc atctttttgt
      421 cccgattgct cttccggcac caggcccagg atatcgacca tgaaagtccg gtaggcaaag
      481 gccagcgccg ccatgtcgtt atcggaaagc ttctctttgc cttctttaac aaggttgatg
      541 atccgggccg cctcgaaaag ctgggcgatc acgatcgggc tgttgaaatc gtcgttcatc
      601 gccgagtagc tattggcaat tatctgtgca attggcccgg taacagttcc gggttccggg
      661 ttccaaagtt ccaaatcttc ataatacacg tttccaaatt tcaacttctg caaattttga
      721 actttgaact ttgaactttg aactattgcg agcgcagcga gcagcttacg cagtcctttt
      781 tcgctcgcct gcagcgcttc gttggagaaa tcgagggtgc cgcggtagtg ggcctgcagg
      841 atgaaaaaac ggatcgtcat cgggctgtaa gctttctcca gcaggctgtg gttgccattg
      901 aaaaaatctt ccagcgaaac ggcattaccc agagatttac ccattttctg cccgttgata
      961 gtgatcatgt tgttgtgcat ccagtaacgc accggcatct tgcccgtcgc agccatcgac
     1021 tgggctattt ccgattcgtg gtgcgggaaa agcagatcca ttcccccgcc gtggatgtcg
     1081 aacgtctcgc ccaggtattt gcatcccatg gccgaacatt cgaggtgcca gccggggtaa
     1141 ccttcgcccc agggcgaggg ccagcgcatg atatgatcgg gggtggcctt tttccacagg
     1201 gcaaaatcga aactgctttt tttctcttcc tgcccgtcga gttcccttgt aaaagccagt
     1261 aattcatcca ctttacgtcc tgaaaggatg ccgtatggat gtttctgatt gtatttttcg
     1321 atgtcgaaat acacagagcc gttgcgctcg taagcaaagc cggcgtcgag gatcttctgc
     1381 accatggcga tctgctcgat aatatggcct gatgcgtgcg gttcaatgct cggattcagc
     1441 aggttcagcc gcgccatgag ttcgtgatag cggttggtgt aatattgcac cacctccatc
     1501 ggttcgagct gttcaagccg ggcctttttg ccgatcttat cgtcgccggc atcggcgtct
     1561 ccttccaggt gcccgacgtc ggttatgttt cgcacgtagc gcaccttgaa gccgatatgt
     1621 ttcaggtaac ggaacaccag gtcgaaagtc acagccggcc gggcatggcc caggtgcgga
     1681 tcgccgtata cggtgggacc gcagacgtac atcccggcat ggcccggtac cagtggctcg
     1741 aaaagttcct tcttccggga aaggctgtta tagatataaa gttgttgcat atcaagtaag
     1801 attccaggca aaaatagtga ttaatagcaa tcggtttatc cgatttcgtc gagtggtcct
     1861 gtaaaactgt ctggcacttt gcttttaaac tgatgcttcg tgcgcctttg tgaatatcct
     1921 tcgtgcgcct ttgtggttaa atcaatcctc cgatctaaga aaaaaccttc gcttgacgat
     1981 gatgtcatca aacaacaaca cacgcaattc ataatcaccc ggttgccgtt tatccggggt
     2041 aatggataca gaaccattta atgtgacgac agtatccgca ggattgacca tcatcttttt
     2101 gtcatagaac gattttccat ccgggccgta ccattccatc cggaagggca attcatcatc
     2161 ttcataaata cccatattcc taaggttaac ccgggcccgg aggatccctt tcttcctgat
     2221 ctcaaatacc gtatcgatgc cattcatctc ccctgtttct ttatccaaac ttttatagaa
     2281 gatgatgtca gcctcgatat gattcgctgt tccttcggga accatctcaa actgcttttc
     2341 cgcgatcaga tccctgaaat aatataccct tacggaataa acgccggcat ccctttttaa
     2401 ggggtcgagg gagactgcac tgaccagttt gccggtcgaa tccccagccg ggagatcgat
     2461 ccttttcaga tagaccgacc ggttgccggg cccgatccag tcgatatgga acaaccggtc
     2521 gcgccccgga tccatcaggg aaacacccgg atcaaccaca gccacaacat tctccccttc
     2581 cttcatttca aaaacattgc taattgcagc ctgcctgccc gatttttcgt aataaaagcg
     2641 gctgaacgtc atctgagcag ggatgccatc ccttttttcg gcgcatcctc caaccatccc
     2701 caccatcaaa atcgcaagga ttatcgcgaa accattaatc aaaacataat atttgatact
     2761 ctttatcatc cttaaattga ataacttacc actcatcctg acaaccctcc aaacccttaa
     2821 aaccctgccg tcctgcagtc ttaccgtcct aaagtcttac cgtcctaccg tcctaccctc
     2881 atatcatcct caccacccag ttcctgcttc tttttttcac gttatagaac cacaggcaaa
     2941 gggggatcat cacgaccatc attacagcga atgcgattaa tgtttcggtg accccgccat
     3001 ccctgtaaag gaaatcaata cgtttgataa aaatccccat gatggcgatg tgcattccgt
     3061 agaaaaacag cgccacgcgc ccgggaatgg cgaaaaagta aagaattttc ggggagattt
     3121 tattgatggc taagaagatt ccggtgatga ccagcaccca tccgaagaac cagaggttca
     3181 ggaagaggct tggcggatat ttctgatcga agaagaaaga ataagaaccg gggtcggaga
     3241 atgggaaaat attgccgtag cccctgccca tccgaaccag gagggccagc agaattgctg
     3301 ccgctccgat accgaagctc caggatatct ttgcccggtc gcttttccag gcctgcagcc
     3361 atccgctggc catggccgat cccaggaggg caagggcaaa ccaggggatg accgggtatt
     3421 tgttaaactc ccccgcatcg ataaaggtct gcatcagggc ttcctgccaa cctgttgccg
     3481 gatcatacgg aatgcggagc aaaagcgggt ggagggccag gatcagcagt cccagtgcgg
     3541 ccctgaccat ccaatgatac cggatgacca gggcgagcag gatcatcgag attccaatgc
     3601 aggcgatgat ccccaggtgg cccggtttga attcgctgaa tcctccccag gaggagttga
     3661 cccaggtaac ctgcagcacg accaggaata acccccggat catgatctgc cggatgatct
     3721 ttttcctttc gatccctttt tcgatttgtt ttgtatacga ccaccagacc atggcccctg
     3781 ccaggatcag gaacccgggg gcacagagat aggaggcgta gcgaagggca aactggggcc
     3841 agctgtcgaa aagcggatcc agcggatcga tctggtccca gacggagtta aaataatagg
     3901 aacaatggtc gagcagcatc aggaccacta ccaggccgcg gacctggtcg atgtaatcga
     3961 aacgcgatgg tcccggtgct gaattaatca aggccataag gaatgctaat gatcaacacc
     4021 ccttcatctt catcggccag atagatatac tggtcatcgg cgtcgatacc cagggcatat
     4081 tttgtcttga ccgcgccgat cagctttggc gccgccggat tggtcacatc gatgatctgc
     4141 attccgccaa gctgagccga catatagatc ttattatcat ccaggtaaag gtctttggca
     4201 tatccggcgc tgtcgtaaac tccggccaca aatacaccgg ctgtgtcgga atagtcgagg
     4261 atctgcaggc cggaggtgcc gcatgccata aaagcgagcg accggttctc gtccagaaca
     4321 atcgcttcgg catcgcccgg tgtatcgcac cagccggctt tgggatagat gccataaccc
     4381 tcctggaaat cggaaatatt gtaaatcgtc aatcccattt ccccgccggc aacgaaaagg
     4441 tagttggaat ccgatgaggt ggccagcccg taagcatagc caatggttgc aaactgcccg
     4501 cggacgtcgg gctcggtcgg atagctgatt tcagcgatac ccactccttg ttcactgatg
     4561 gcggtgaaca ggtaatgccc catgatgtgg aaattccgtg ccggtttgac gcccaggttg
     4621 gtaatggcta ccctcggtgt atcgggttgg ctgacattta ccacattgat gccgaatgta
     4681 ttagctgcca gataaaccgc actgtcgcgg atagcgatct tcccggcata accccgaagc
     4741 ttatccgttg taagcgaaat gaaggaagga acggccggat ttgtaatatc gtaaatcagc
     4801 aatccccctt ctccctgggc aatgtagaga agattatctt ttttaacgac gtcgtttgaa
     4861 aatcccgacg acgcaatccg gctgacgatc ttgtacccac catcggacgg tgtaagcgat
     4921 tcgggatctt tcggtttgcc gcatccggcc agtaaaatga cgccggaaac aaataataac
     4981 aggtttttat tcatagttta atcaatttaa aacttgagat tatacactac ctgcaagcca
     5041 acctggtcct ggcggtagtc tttttcttcg gataaatact cgctgttgat ttcggatgac
     5101 gaattggaat tcctcatgaa aaatttatag aaagccccta aagtgaatga cttgctcacc
     5161 tggaggttat aattggctgt aaccaggacg acatcatcca ccctcccggt atgttcggga
     5221 tcttcctcca ggaaatgctc cgaaaaatag acccggttct gataggtggc attcaaatcg
     5281 atttcatgcg ttttctttcg gattttcgga agctcccagt tcagtcccag ggcaaaggcg
     5341 tattcgttgt aggtggcatc ggcatcgtcg gctgtttccc gggtctcgcc cggttcgtcg
     5401 taacctttgg cgtcagaatg ttcgaactcg taagctgctt cgatcttcac ctttttgtga
     5461 agcggttgcg aaacagaaat cccgaaaata tagctgttgc agtcatattc cgtataatgt
     5521 ttattatgaa aataacgggc caggtcgaac gataatctta cccgggtgct tttcaggaaa
     5581 gtgttctgaa accagaaacc gtaattatcc tttgcaaaag agaaagggac gaaagtttcc
     5641 ggggtatagc cgtatatctc cacccggtcg gcatccctaa agtgcctcac ataaaagcgg
     5701 ggaatgtgag agtaggaaac cctgaagctg gctttcttgg tgacattttg tgtgagcccc
     5761 agggtgaaat aataccagtt tttcacgccg ttggtggcat acatgttact gtaaatgctt
     5821 ccgttgatcc gggttttaag gtctttgaag atccggtatg ttgcagacag gtcggcagcc
     5881 gtgatgaaca cgatatcatc gtatgtcttg atgtgaaacc tgccggaatc ctggcgattg
     5941 atgaaacgat caatatactt gccggaatat ttcaggatat tgtcgtcgta catcgagccg
     6001 aatttaatct cggcctcgaa atgtttctcc tttttggaag atttcttttg cgcggctgcg
     6061 gtctgcattc cgaatactaa aaaaagtatg atcagaaagt aggtaggtaa gcagcatcga
     6121 taatatttct gtatgaatat tctcattttc agttgttttt attcttcgag tccgacatct
     6181 ttccctggaa tcagcaatcg tccgagcaaa gaatttttat cctggtcgag cgggcagatt
     6241 tcatatagat gttttccgtc ggggatcagg ataacaaatt cgcaggccgt tccagggatg
     6301 agattgtcga tatctttata aacggctaca tccgacactt tgctgttcaa ctggtaggta
     6361 ttgatgaccg atccgttttc cctgacctgg accctgtaat tgatccgtcc tttcatccca
     6421 aaatggtgct ccgttcggat caggacgcgc aattcggtcg ggccgatgat ctcaacacgc
     6481 agcggtttct ctgctgtaaa ccggtaataa ggcgtggtgg tttcacggga gatgagatcg
     6541 acgggctcgg aagggcgcag gggggaaaaa gccatccaat cctgcttttt cagcttagcg
     6601 ggggtaaaaa cataccgggc agccacattg gcggtaatat ccttcaaccg gaattcaacc
     6661 gtatgaaatc cccgcgaaag ttcgatttcg aatgttttca gctctcccgg gacgccaagg
     6721 gtgccatcgg tgtaggttgc attccgtgaa cgttcgatcc ctgaaaccga aacacttttt
     6781 tgagtcccgc catcgaccgt atagataacc gtatagcgtg cctggacctg gtcgcccggc
     6841 cggaactgcg cacgggtatg gactttcagc acccccggcc cctggacggt aaccattgcc
     6901 gcatcggcat aactcagcgc ataatagttc ctgttcttcc cggcgctcac agtcgtaatc
     6961 ttcttcacaa atcccgaagg cttaatggtc ttcgattccg tctgggcaca gatactgccg
     7021 gttaaaagca ataaaaaacc gaaaactcca aatttcttaa tcatcatatt atcaaataaa
     7081 actctcaata caaatcgaaa atcgaccatc gacaatcgac aatcgaccat catatctctt
     7141 caatcatctc atcttcagcc tgctgcttcc tctgtcccgg aacgacaggc acaacatccg
     7201 ttcccagctg gtgccgtttt cgttccgcaa ggtaaataaa aatgatgctg gtgacgagga
     7261 tggccagagc aatagtcgtt acaacgagat catatttttt ctgaataaaa atgcctcctt
     7321 cacccggttc cggcatgggc accggattgg ccggcagtcc gtattttgct gccagctggt
     7381 cgatgttcag caggtgaatg atggggatcc ctttccggcc catctggatg atcacaccgt
     7441 gcaacgggta attgctcatg ggaaggcgtt cggtaagccc cgaagggatg agctgcccat
     7501 ttaccgtatt gccgaggctg gctatccctc cgccgacatt gataaacgcc ctggctgcat
     7561 tctttttgcc ctgttgctcg taaatttcca ttctcctggc aatggagttt tcgaggtgtt
     7621 tctcattaat caattcaacc cggttccttt caatggcgct aatgataaga ttccggcctt
     7681 cggggctcag gccccggccg atgtcattcc cgccgccgat ggaagcggct accgatttgt
     7741 agtgaaaaat gcctgcatca ttcagcagtg tctctatatc gagccaggta aaatagggat
     7801 cattggctcc gaaattggat gccccgacag aggtgatcac ggttggttgc agtttgagcg
     7861 tctcaatggc cgccattacg gagatattca tcccggggaa agaccccgag aatgctatgg
     7921 ccaccggatc gccttcttcc agccccgcct ctttcagcaa ctgaacaatc actgcggcaa
     7981 agttcgggtt ggtagcagat aattttgatt cgacatttcc ctggtcggtc gtgatcatgg
     8041 tgtattcctg tccgatcagg gccgtttgat tgggatcatt cacatcatcc agaaaaacac
     8101 ccttctgcag gcgatattgt ttgatatgac cggctgcagc ctgggagagc cgggctgcct
     8161 caagtttttc attgtaataa tcctgtttga catctttctt tccattctcc actgcgataa
     8221 aagccagcaa agccagaagg aagaggaggc ccagcacgag gttcgaagtt gctttgaatt
     8281 taaacatatc acctcctatt tgggcagtaa agcgcccgca aagatcagga taagaaccag
     8341 gcggacgagt acggatgaaa caaacagtac gctgatcgtt ttgaagattc cctgtttggc
     8401 aaaccagtgg gcgatcagcc ccgggatgac ccagccgacg gcttcgagtt caagcgttga
     8461 gcttcccagg ttaaaggtca ggaaatgccg ggtgaaaata gccagcaaac aaccgatcaa
     8521 caggctgagg accatctggc gcctgccata caaaaaggtg taattgccca gcactttaat
     8581 gatcaggtaa gtcaccaggc tgaccaccag gattccgata agccggtcgg ggtgcgacag
     8641 ttgcagggcg atatagcccg gaacgactat cccgccggcc gccagtccga atacttcgta
     8701 tgaaagaagg ctgaggacca ggccaagggt tatggctaat tcaatcataa attatatact
     8761 cctgtgttca aagaattcga cgagacccga tcccattccg cccatattgc cgatcgcaac
     8821 aatggtggcc gtgtggtcgg tcagagccag caaccggtcg aaaacatttt ccgatgttgt
     8881 ccatccgacc gttacgactt tccgggcagg aatgccatag cgaatagcca tgctctccac
     8941 aacatcggtc gattgcccga tcaacacaag ataatcgacc tccttttcga gccggtaagc
     9001 caccatgccg gccagttgct ctgcccggta cagccggtcc tgccgggtat tcagcaacac
     9061 gatccgtttc ccttccattc ccgtttcatc gctgatcttc cgccagacca ggaaggtgga
     9121 atcgggatca ttggcggcaa aggcattgta aaagaccagg tttttccgga aggcttccac
     9181 cctgaaaacc atcagggcgc ccgcatctgg aacagctttg tacatcccct gcaacgccgt
     9241 attcctatcg acgccggcat gcaggcaaac ggccagcgcc agcgaaacat tctcccggtg
     9301 ttcaatatac ctgaaacctt gcatctcctc atgcgaaaca atttccggat ccacaaaatg
     9361 ggtcacggta ttccgtttat cggccatttt cttcaggagg tcgggaatca cgttttcaga
     9421 ggtgaaaaga tgttccttcg cgggtatggt ccgacccagt gtctcagcaa tttcaccgag
     9481 agtatggccc atcacatcgg tatggtccat ccggatattg gtcatgactc cgacattgga
     9541 gtgaatcatc cggtgttccg taatcgtctg atattgtggc tgcaatgcca tacattccat
     9601 caccagcgcc ttgacctttc gggatgcggc aaaccggacg atcgataatt gttcgatgat
     9661 attggccgaa gctttccggt gaatgtaggt ttctgtgcca tcttccagga taagccgggg
     9721 aaacgttccg gtcacttttg tgatattggt tattccgcct tcccttagcc ctgccccgat
     9781 cagccgcgtc acactcgatt tccctctcgt cccgttcaca tggatcctga tcgggatgga
     9841 tgaaatcctt ttctggtgca gaaaatattc gatgatcccg aatatgactg ccatcagtac
     9901 cagggcaaga agaatataaa aactatgcat taataacgtg aactatctca ataagaacaa
     9961 tttaacggtc tcccggaaat tttctgattt tatttggcag aaataaaccc cggcacttac
    10021 cggtaaacct gcatcattgc atccatccca cagaatctcg taggttccgg cgttttgccg
    10081 ggcgctcttt aatatcctca ccgttctgcc ctctacatcg agaattttca ccgatacggc
    10141 cgccggattg tcaattgtat agcttatccg cgtcgactcc acgaacggat tcgggaaaca
    10201 gctgagatga tccgacgttt ccatcgatcc ctctttcctg tcatcaatcc cggtgataat
    10261 tgaggcttcc ggcggttcgg tcgtgaactt gatggaaaac tcgtttttca ggatatttga
    10321 gccgggatta taggcgtcgt tgaacaggta tagcagaccg atattctgcc cctggttttc
    10381 gatgccgatg gtgctgcttt caggtgcccg aacctgtttg taattaaata cgatctcacc
    10441 gtcacctgtc aatgtgggat aaaaatcggg atcataaagc accgcctgga aaatctccac
    10501 attgggttcg gaaccgaaat cgttccgggc tatgctgtcc cattcgatga taacccggtg
    10561 attggagaaa tcgttgtaat aataaatatt cccctccacg gtttcagcgt cataaagatc
    10621 gtcccagaaa ccggccacca tgcagttgat attatcgatg ttcggaaggg tggtgttgac
    10681 cggtgccgtc tggttaccgt ttccgaaagc tatccagccg tcggtactga tccggagctg
    10741 ggcatattca acgccataat acttgaatgt aaaggggaga ctgactgttt cggtataatc
    10801 tcctattcct gaaatattga tcggtgtacc ggcctgatcg atttcgatcc aggtataacc
    10861 gggtgcctgg tcgaaaatat catcactgga gtatgcataa tagccatagg catccggacc
    10921 cgtgtagtcg gaaggcaccg ggtgcgatac aggcaggttc agggtgagat tcttctgata
    10981 tggataattc ccgttggaag tcgtcaattg aaggttgaac tgggcgaaat aatcagccgg
    11041 gcaggaaggg tcaattttta ccacaaaata attgtaaaaa ttaatggctg tttcattgat
    11101 tgcaatcgtt ccaaatgctc ccgctccatc ctcaatatca ataaacggat catcggatgt
    11161 caaaataccc accacatctt ttgccaggtc attgccggca ttgtgaacgg ctataaaaag
    11221 cttgaccgtt tcgcccgggt ccatccggta attcggttcg gcggctccct ggtcattcac
    11281 ataaaatccc tcatccaaaa gattgcaccc gttgataaca atatcataac tgtaatccca
    11341 ggaattttcg gtactggtaa catgcagctg aaccgggatc aactgtccga cagggcaatt
    11401 ttcagcaacc cgcagcatga atgggtttcc tgtaatcttt gccccggagg caagatttcc
    11461 gtagttgacc ggcacggtgg tcaatacctg catataatca ggcatacccg atgtcagtgt
    11521 tgccatgaca ttgtttgcag tctggcttcc ccagtttttc agtgtaaagg tggctgtgcc
    11581 gttttcgttt gggtcgataa ggccgtcgag gttgccatcc aggtcgtcaa gcgccggtcc
    11641 tccatccggc tcaacgagtt cttcgggctg gatcacgatg atgtcggcct ggaaaggata
    11701 aacatttttc cccctgacgg tcagttttaa agtatccgga gtctccggga caatatccat
    11761 gtagactttc ccctcactat cacaatatcc tgtcgcaaag atctcgtcgc cggtaaggca
    11821 cacctgtgca ttagccgccg gctgaccgga tgcggtgaaa ttcaccgtga tctccacctg
    11881 gtcctgtccc accaggatgg tttggggata atcaacggta acggccaggg gaacatccct
    11941 ccagatatgt atgctggga
//
LOCUS       DAOPNH010000070        11813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5722_length_11813_cov_6.261354, whole genome shotgun sequence.
ACCESSION   DAOPNH010000070 DAOPNH010000000
VERSION     DAOPNH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_11813_cov_6.261354"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKZ74_05275"
     CDS             <1..380
                     /locus_tag="PKZ74_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPM92202.1"
                     /translation="LASFLLNITHPDQLVNHYLKGNLFENLIIIELLKKRFNNGLTNN
                     LWFFRDSHGNEIDLIPEALNLRAVEIKSSRTFSTGFTDGFKWFQKISEVQNRDCFVIY
                     GGDESYSFNDFHVLSYKNLDSLM"
     gene            441..911
                     /locus_tag="PKZ74_05280"
     CDS             441..911
                     /locus_tag="PKZ74_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HPM92203.1"
                     /translation="MKIQIIFTVIIISISMMANAQNTTGQIPVMIENESRYDFAATVD
                     TLSDIIVSGGWKVITSHDLRETMKKNGKEVLPVKVMEICNPNLAYQILSRDELRDASP
                     MLPCRISVYEKADGKTYVSRMNAPAFAGMIGGDAAKTIVEAFEAAEGFLKPVIR"
     gene            945..1322
                     /locus_tag="PKZ74_05285"
     CDS             945..1322
                     /locus_tag="PKZ74_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HPM92204.1"
                     /translation="MSLKKILFVVLGTLSLAIGIIAIFIPGLPTTAFLLLTAALYMRS
                     SDRLYRKLLANRYVGPYIREFRENKGMTRRMKIHAIGTMWVMISISCIFFVDSWTIRL
                     VIIGVGLIGTVVMGFFVPTSAKP"
     gene            1341..2405
                     /locus_tag="PKZ74_05290"
     CDS             1341..2405
                     /locus_tag="PKZ74_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HPM92205.1"
                     /translation="MSKLFTPLTIKSVTFRNRLAIPPMCQYSAENGYANDYHLVHYGA
                     RSIGGAGLIIQEATAVSPEGRITPGDLGIWDNGHVDKLQAIASFIAAYGAVPGIQIAH
                     AGRKAGCAKPWHGGKQLSPAEGGWQTVAPSGIPFAEGENAPAALDDAGIRKVVNDFRN
                     AAGSAAAAGYKVLEIHAAHGYLIHQFLSPLANKRTDSYGGSFENRIRLLLEIVEAVNG
                     NWPQHLPLFVRLSTTDYIDGGWNPDETVRLAVILKSKGIDLIDTSSGGMVPYAKIPFG
                     PGYQAGFAEKIRKEAGIMTGAVGLITEARQAEEILEKGQADLVLVGRQWLRDPHFALN
                     AAKVLGDDIAWPEQYLRAKS"
     gene            complement(2416..4776)
                     /locus_tag="PKZ74_05295"
     CDS             complement(2416..4776)
                     /locus_tag="PKZ74_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM92206.1"
                     /translation="MNTKVIKFIVTQVFIVFSLAGWSLFAQEYDTITNWDGIEREWYV
                     STSGSGKAANPAPDEMNGSAHCFRFVTDEGSFEYMITELDELVDFSLCPRYRIKVLAP
                     ESGGNITLKFENYNNSFWQEIVLTPVPGQWTDLEYDFSGLVYDGLIKMVIFPDFQGNE
                     PGNYWYIDDILREACAEPEPLQLETNLPIIVINTFGEPIVDEPKIEAHMGIIDNGPGE
                     INSLDDPFNNYDGMIGIETRGQSSQMFPKKSYGFETRDAEGENLDTSLLGLPEENDWV
                     LYAPYSDKSMLRNFMSFEMGSRMGNYCTRQVFCELVINDDYKGIYTLMEKIKKDDDRV
                     DIATLNPDEISGDDLTGGYIIKVDKLDWDFEFDSDGWRSDPDPPYPNAMDIIFQYYYP
                     EADEMPSQQKIYIRDYITDAENALTSFTFSMPEIGYMQYFDVLSYVDFMLLCEISKEV
                     DKYRYSTYMYKEKDSDGGKLFAGPLWDFDLGYGNVDYWPEGLDYTGWLYYMVNPYEWS
                     IMFWWKRLMEDPYFRNMAKTRWEYLRQERLSDEDVHQMIDSVVNLLEDARVRNFERWP
                     ILGEYVWPNYDWFGNTYEDEVDYFEDFLFNRLQWMDGNFTGNLLFPWASISAQENKIS
                     LALYDDFFATRVLKKSWFTLNDAPAGVTVLTATYKDASHCELYLSEDISGAGDLSVTV
                     SHEAINYWLDLTSNRLEATGAGELPGENNSTRVYWENQQLVVINDHPLILPGNAEIFN
                     LAGQKMACFGIDPENVNRFSLDLKPGLYIVTLKNDHSTSTHKIIVR"
     gene            complement(4783..7500)
                     /locus_tag="PKZ74_05300"
     CDS             complement(4783..7500)
                     /locus_tag="PKZ74_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPM92207.1"
                     /translation="MNLSILKIFFFSLLISALNHSFAQGYLHTSGKYIHDGDGNELIL
                     RGIGTGNWLLNEGYMMKTADFAGTHTQFRTLLTETIGEENTEIYYQHWLDNHFTRHDV
                     DSMKNWGFNSVRVAMHYKWFTLPIEQEPVTGQDTWFEEGFTRIDSLLDWCGDNQMYLI
                     LDLHGAPGGQGHDRNISDYDPDFPSLWESAENRRKTAALWKRLAERYAEEPWIGGYDL
                     INEPNWDIPGGSLLRQTYITITSAIREVDTNHMIIIEGNWFANDYTGLTPPWDDNMVF
                     SFHKYWNYNTQESIQWMINLRNTYNVPIWLGETGENSNSWFADLVELAENNKIGWSWW
                     PVKKAGINNVLMVPESPLYNLLLSFWENGSPALTQQQAFEAVLDWAENHKIENCTVQR
                     DVIDALLRQPHENSALPFRDHQVSQVINMVDYDLGKCSVAYWDTDTANYHLNTGSFTD
                     WNRGWAYRNDGVDIEPCQDDFPGTNGYGIGFTAAGEWLQYTVASDTAAAYKMVIRSAS
                     ESNPGSVHLTVNGIDASPSIQMPVTYGWQNWLPAIAENIIIPAGINQIRVCFDKGGSN
                     YSLLQFSDPAPVAGVPFQFVAGTTNPDGSALIITLNKDITGFDADISDFDVLADEVPV
                     TVNAAMVSTSSNRKLILYLADKLRFGQTVTLSYSGTGINAGDQALETFTGKPVKNTLP
                     HRYLLPALIQAEDYIVNSGYQLENCTDINGGQNIGYANNGDYLDYFVSVPQAGEYIFR
                     LRAASLYSNGSISLRLGDGSSFTSLKTINITQTGGWQNWITLPVTIDLPQGDFTLRIL
                     SLSGEYNINWFEISLPAGENQYGPIRRFQVFPNPSDGKIRVEADFAGNSGARLKIFSS
                     NGKIIYNRDFTETYDFSQNIDLAYANPGIYFLCLTTASGSSFTRKVIIN"
     gene            complement(7567..9312)
                     /locus_tag="PKZ74_05305"
     CDS             complement(7567..9312)
                     /locus_tag="PKZ74_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPM92208.1"
                     /translation="MKKLTPEIRKLFHDHRNTLAVIKLYAQLGYHDQDIPESAREFFN
                     TISGQTAEAVRSIEDLRLLLEEDLFPGDQEANPLLRESDKQLEVLLSNLPGMAYRCKN
                     APGWPMEFISEGCLDLTGYDPEDFIMGKKLQYEDIIHPDDRNYVNDTIQEYVVKDMPF
                     TLEYRIKAKDGTERRVWEKGRQVLGNDNTERRLEGFIMDITERIHVEEELKRNQEKIM
                     SVFRVSPSGIGMVINRMITEINDRICEMTGYSQDELIGQNALILYPSKEDYDYVGTEK
                     YRQIEKTGIGVVETRWKRKNGTVIDILLASTPLVNHDNSQGVIFTALDITNFKKTEAE
                     KRRIEEQLFQARKLEVIGKLAGGVAHEFNNTLQVINTLTELSIMKLDQDHPVAGHLDQ
                     IRTSVRQSSAFIGQLLTFARKQPFNPVIINLNDYIGSILDMLHRLIGDRIKVNWIPGI
                     DLWQTRIDPVQLNQVLINLTINARDAIGDNGTINVITGNEKITVAASKKLHGSRPGEF
                     VRLTIRDNGCGMEKEILQQIFDPFFTTKPVGKGTGLGLSTVYGIVDQNHGFILADSQP
                     GKGSTFHAYFPRSNA"
     gene            complement(9366..9737)
                     /locus_tag="PKZ74_05310"
     CDS             complement(9366..9737)
                     /locus_tag="PKZ74_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM92209.1"
                     /translation="MVDDNLPMRSALSETLKFLNYKTVEAENGQQALEIIEKSCSQPE
                     TGEEPELALILCDWSMPILDGRGLFNEMKKRNLDIPFVMLSGYMTPAEAEEMTGTGLA
                     GWLQKPADIRQIANLLEKILH"
     gene            complement(10083..10712)
                     /locus_tag="PKZ74_05315"
     CDS             complement(10083..10712)
                     /locus_tag="PKZ74_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92210.1"
                     /translation="MKKLDIAIVDDHTLFRQGLSAALSSAKIINRIFEASNGEEFLEL
                     IKTQTPDVVLMDISMPVLDGIEATRIAVSGNNQLKIIALSMHNDIEHFQDMIEAGVVG
                     FLSKDSALEDVSAAIESVANGNKVFSQDLMYSLVRKMNKEKSQTDLITEREREILQRV
                     SSGKSNNEIAAELNISKRTVDKHRENILAKTQTKNTAELIMFALKNKLI"
     gene            complement(10727..11710)
                     /gene="dusB"
                     /locus_tag="PKZ74_05320"
     CDS             complement(10727..11710)
                     /gene="dusB"
                     /locus_tag="PKZ74_05320"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPM92211.1"
                     /translation="MVKIGPIDAGEFPILLAPMEDVSDPPFRYVCKLFGADITYTEFI
                     SSEGLIRDAAKSLKKLEFTEFERPIGIQIFGHDIESMVKAARYAEEANPDIIDINYGC
                     PVKKVAGKGAGSGILNDIPKMVKMTEAVVRAVNKPVTVKTRLGYDNDRKEIVEIAERL
                     QDVGIQAITIHGRTRVTRSSEPADWTLIGKVKENPAMHIPVFGNGDVFTPQAAKFFKE
                     NYHVDGLMVARGSYGNPWIFRDIKHYLATGRILSAPDIHERVRICRIHLEKSVEWKGE
                     RVAVNEIRKHYSDYFKGFPDFKRYKVLLMEESSSEKINQILNDIEHYYSYI"
BASE COUNT         2901 a         2883 c         2798 g         3231 t
ORIGIN      
        1 gcctcgcatc gttccttttg aatatcaccc atccggacca gttggtcaac cactacctaa
       61 agggaaattt atttgagaat ttgataatta tagagctttt aaagaagcgg tttaacaatg
      121 ggttaaccaa taacttatgg ttcttcaggg atagtcacgg caacgaaata gacctgatcc
      181 ctgaggcttt gaatcttagg gcggtggaaa taaaatcttc acgaacgttc tcaaccggtt
      241 ttactgacgg attcaagtgg ttccagaaga tttcagaggt gcaaaaccgc gattgctttg
      301 tgatttatgg tggcgatgaa agttattcct ttaatgattt tcatgtacta tcttataaaa
      361 acctggacag ccttatgtag ctttgaacct ttctccgtcc gcgttgttaa taatctttaa
      421 ggttcaataa aatccgatcc atgaaaattc aaattatttt taccgttata attatatcca
      481 tttcaatgat ggcaaatgca cagaatacaa cgggtcagat tcccgttatg attgaaaatg
      541 agagtcgcta tgattttgcg gcaacagtcg ataccctttc agatatcatt gtttcgggcg
      601 gctggaaagt gatcacctcg cacgatctcc gggagaccat gaagaagaac gggaaagagg
      661 tcctgcccgt caaggtgatg gagatatgca acccgaacct ggcttaccag atcctttcca
      721 gggatgagct ccgggatgcg tcgccgatgc ttccctgccg gatttcggtt tatgagaaag
      781 ccgacggcaa aacgtatgtt tcacggatga atgcccccgc attcgccggc atgatcggtg
      841 gcgacgcggc aaaaaccatc gtggaagctt ttgaagctgc cgaaggattt ttaaagccgg
      901 tgatccgcta gtttttgcta acttgcgtcg catattgcgg gcaaatgtcg ctgaaaaaaa
      961 tcctttttgt cgttttaggt accttatccc tggctatcgg gatcattgcc atttttatcc
     1021 ccggtctgcc cacaaccgct ttcctgttgc tgacggcagc gctctacatg cgcagctccg
     1081 accggcttta ccggaaattg ctggccaacc ggtatgtcgg tccctatatc cgtgaattca
     1141 gggaaaataa aggaatgacc cggcggatga agatccatgc catcggcact atgtgggtga
     1201 tgatctccat ttcgtgcatc tttttcgtcg attcatggac catccgcctg gttatcattg
     1261 gcgtaggact gatcggcacc gttgtgatgg gatttttcgt tccgacatcg gccaaacctt
     1321 aactatctca aatttcagac atgtcaaagc tatttacacc cctgaccatc aaatcggtaa
     1381 ctttcaggaa ccggctcgct atcccgccga tgtgccagta tagcgctgaa aacggctatg
     1441 caaatgatta ccatctcgtt cattacggtg cacgatctat cggaggcgca gggctcatta
     1501 tccaggaagc caccgccgta tcacccgaag ggcgcatcac cccgggcgac ctgggaatct
     1561 gggataacgg gcacgtggat aaattgcagg ccattgcaag ctttatcgca gcttacggcg
     1621 ccgttccggg catccagatt gcccatgccg gacggaaagc cggttgtgcc aaaccctggc
     1681 atggcggaaa acagctcagc ccggcagaag ggggctggca aacggtcgcc ccttccggca
     1741 ttccttttgc cgaaggcgag aatgcgcctg cagcgctgga tgatgcagga atccgcaaag
     1801 tggtgaatga tttcaggaat gccgccggaa gtgctgccgc tgccgggtat aaagtgctgg
     1861 aaatccatgc ggcccacggt tatctcattc accagttcct gtcgcccctg gccaacaaaa
     1921 gaaccgacag ctacggtgga agcttcgaaa accgtatcag gctcctcctg gagatcgtgg
     1981 aggcagtgaa cgggaattgg ccgcagcacc tcccgctctt tgtcaggctt tccaccacag
     2041 attatatcga tggcggctgg aatccggacg aaacggtcag gctggccgtg atccttaaat
     2101 ccaaagggat tgacctgatc gacacctcat cgggaggaat ggtcccttat gcaaagattc
     2161 ccttcgggcc cggctaccag gcaggctttg cggaaaagat caggaaagag gccgggatca
     2221 tgaccggcgc tgtgggtttg atcaccgaag cccggcaggc ggaggagatt ctggaaaaag
     2281 gccaggccga cctggtactc gtcgggcgcc agtggctgag agatccgcat ttcgcgctca
     2341 acgcggctaa agtattggga gatgatatcg cctggccgga gcagtacctg agggcgaaga
     2401 gctgatcagg caaacctacc ggacgatgat tttatgagtg ctggtgctat ggtcattctt
     2461 cagcgtcaca atataaagtc ccggttttaa atccaatgaa aaccggttaa cgttttcagg
     2521 atcaattccg aaacaagcca ttttctgccc ggcaagattg aagatttcag cgttgcccgg
     2581 caggattaaa ggatgatcat ttataacaac caattgctga ttttcccaat aaaccctggt
     2641 ggagttattt tcgccaggca attccccggc cccggtcgct tcgagccggt tgctggtcag
     2701 gtcgagccag taattgatgg cctcgtgcga aaccgtaacg gaaagatcgc ccgcgccgct
     2761 gatatcttcc gagagataaa gctcgcaatg ggatgcatcc ttgtaagtgg ctgtcaggac
     2821 agtaacaccg gcgggggcgt cgttcagcgt aaaccagctt tttttcagta cccgggtggc
     2881 aaaaaagtca tcgtaaaggg ccaggctgat cttgttttcc tgcgctgaga tgcttgccca
     2941 gggaaaaagc aggttgccgg taaaatttcc gtccatccat tgtaaacggt tgaaaaggaa
     3001 atcttcgaaa taatcgactt catcttcgta tgtattcccg aaccagtcgt aatttggcca
     3061 tacgtactcg cccaggatcg gccaccgctc aaaattccgc accctggcat cttccagcaa
     3121 gttaaccacc gaatcgatca tctggtggac atcctcatcg ctgaggcgtt cctggcggag
     3181 atactcccag cgggtttttg ccatgtttct gaaataagga tcttccatca gcctcttcca
     3241 ccagaacatg atgctccatt cgtagggatt taccatgtaa taaagccatc cggtatagtc
     3301 caaaccctcc ggccagtaat cgacattgcc atagcccagg tcaaaatccc agagcgggcc
     3361 ggcaaacaat tttcccccgt cgctgtcttt ctccttgtac atatacgtgc tgtaacgata
     3421 tttgtcgacc tctttggaaa tttcacacag aagcataaaa tcaacatatg aaagaacgtc
     3481 gaaatactgc atataaccta tctcaggcat ggaaaaagtg aagcttgtca gcgcattctc
     3541 agcatcagtt atataatctc ggatgtaaat tttttgctga gatggcatct catcggcttc
     3601 cgggtagtaa tactggaaga tgatgtccat cgcgttcgga taggggggat cgggatccga
     3661 tctccagccg tcggaatcga actcgaaatc ccagtccagt ttatcgactt ttatgatata
     3721 acctcccgtc aggtcatctc ctgaaatctc gtcgggattg agggtggcaa tatcaacccg
     3781 gtcatcgtct ttcttgatct tttccatcag ggtataaatc cctttgtaat catcattgat
     3841 caccagttcg cagaaaacct gccgggtgca gtagttgccc atcctgcttc ccatttcgaa
     3901 cgacatgaaa ttccggagca tcgatttgtc gctataaggc gcatagagca cccagtcgtt
     3961 ctcttcgggc aaaccgagca ggctggtatc gaggttttca ccttcggcat cacgggtttc
     4021 aaaaccgtac gattttttgg ggaacatttg cgatgactgc ccccgggtct caatcccgat
     4081 cattccgtcg taattgttga aaggatcatc caggctgtta atctccccgg ggccgttgtc
     4141 aatgattccc atatgggctt cgatctttgg ctcatcgact atcggttcgc caaaagtgtt
     4201 gatgacgatg atcggaagat ttgtttccag ttgcaacggt tcgggctctg cgcaggcctc
     4261 tctcaggata tcatcgatat accagtaatt cccgggttca ttgccctgga aatcaggaaa
     4321 aatgaccatc ttgatcaatc catcgtaaac aagacccgaa aaatcatatt ccaggtcggt
     4381 ccattgcccc gggaccggcg tcagcacgat ttcctgccag aaggaattat tataattctc
     4441 gaatttcagg gtgatgttac cgcccgattc cggcgccagg actttgatcc ggtagcgtgg
     4501 acacaggctg aaatcaacca actcatccag ttcggtgatc atgtattcaa aggaaccttc
     4561 atcggtcaca aaccggaaac agtgcgccga gccattcatt tcatcaggcg cgggattggc
     4621 tgcttttccg gagccagagg tagagacgta ccattcccgt tcaatcccgt cccagtttgt
     4681 tatcgtatcg tattcctggg caaagagtga ccatcccgca agggaaaaaa caataaaaac
     4741 ctgtgttaca ataaatttaa tcacttttgt attcattccc gatcagttta tgatgacttt
     4801 gcgtgtaaaa gacgatcctg aggcggtggt taagcagaga aagtagatac ccggatttgc
     4861 atacgccagg tcgatattct ggctgaaatc atatgtttcc gtaaagtcac ggttatagat
     4921 tattttgccg ttggaggaaa atattttcaa ccgggcgccg gaattaccgg caaaatcagc
     4981 ctcaacccgt attttaccgt cggagggatt agggaaaacc tgaaaccgcc tgatgggtcc
     5041 atactggttc tcacctgccg gcaggctgat ctcaaaccag ttgatgttgt attcacccga
     5101 taaggagaga attcgaaggg tgaagtctcc ctggggcaga tcgatagtca ccggaagggt
     5161 gatccagttt tgccagcctc ccgtctgggt gatattgatg gttttgagcg atgtaaagga
     5221 actgccgtcg cccagccgga gcgaaatgct tccattggag tagagggaag cggccctgag
     5281 ccggaaaatg tactcgcctg cctgcggcac cgagacaaag taatcgagat aatcgccgtt
     5341 attggcatag ccgatattct ggcccccgtt gatatcggtg cagttttcaa gctggtaacc
     5401 ggagttgacg atgtaatcct cggcctggat cagcgccggc agcagatagc ggtgtggcag
     5461 ggtgtttttc accggtttcc cggtgaatgt ttccagcgcc tggtcgccgg catttatccc
     5521 ggtccccgag taggagagtg tcaccgtttg cccgaaccgc agcttatctg cgaggtagag
     5581 aatcagtttc cggttgctgg aggtgctgac catggcggca ttgaccgtta ccgggacttc
     5641 atcggccaga acgtcgaaat cggaaatatc agcatcaaag ccggtgatat ctttgttcag
     5701 cgtaatgatc agggccgaac catcgggatt tgtcgtaccg gcgacaaact gaaaggggac
     5761 gccggccacg ggggccggat cactgaattg taaaaggctg tagtttgatc cgcctttatc
     5821 gaagcatacc ctgatctggt tgatacctgc gggaataatg atattttcgg ctattgccgg
     5881 cagccagttc tgccatccgt aagtcacagg catctggatg gaaggggaag cgtcgatacc
     5941 gttaacagtg aggtgtacac tgcccggatt ggattccgag gccgagcgga taaccatttt
     6001 ataagcagcg gctgtatcag aagccaccgt gtattgcagc cattcgccgg cagccgtaaa
     6061 cccgataccg taaccgttcg ttccgggaaa gtcatcctgg cacggttcga tgtcaacgcc
     6121 atcgttccgg taggcccatc cccggttcca gtcggtaaat gaacctgtat tcaggtgata
     6181 attggctgta tcggtgtccc aataagctac cgaacatttc cccaggtcat aatctaccat
     6241 gttaatcacc tggctgactt gatgatcgcg aaaaggaagg gcgctatttt catgaggctg
     6301 gcggagcaat gcatcgatta catcacgctg tacggtacaa ttttcaattt tatgattctc
     6361 ggcccagtcg aggacagctt cgaaagcctg ctgctgtgtc aaagccgggg atccgttttc
     6421 ccagaaggaa agcagtagat tgtagagcgg gctttcaggt accatgagca cattattgat
     6481 ccccgctttc ttcaccggcc accacgacca gccgatctta ttattctccg ccagttcgac
     6541 gaggtcggca aaccaggagt ttgaattttc accggtttcg cccagccata tcgggacatt
     6601 gtacgtattg cgcaggttta tcatccattg gatcgattcc tgtgtattat aattccagta
     6661 tttgtgaaaa ctgaaaacca tattgtcgtc ccagggaggg gtaagcccgg tatagtcatt
     6721 ggcaaaccag ttgccttcga tgatgatcat atggttggta tcaacttcgc ggatggcgct
     6781 ggtaatcgtg atataggttt gccggaggag agaacctccg gggatatccc agttgggttc
     6841 gttgatcagg tcataaccgc cgatccaggg ctcctcagca tatcgttccg ccagccgctt
     6901 ccacaaagct gctgttttcc tcctgttttc agcgctttcc cagagggaag ggaaatcggg
     6961 gtcgtaatcg gagatgttgc ggtcgtggcc ctggccgccg ggggcgccat gcaggtcaag
     7021 tataaggtac atttggttat cgccgcacca gtcaagcagg ctgtcgatcc gcgtaaaacc
     7081 ttcttcaaac cacgtatcct ggcctgtaac cggttcctgc tcaatgggca gggtaaacca
     7141 tttgtaatgc atggccaccc tgacgctgtt gaatccccag ttcttcattg aatccacatc
     7201 atggcgtgtg aagtgattat cgagccagtg ctggtagtat atttccgtat tttcctcccc
     7261 gatggtctct gtcagaaggg tccggaactg ggtgtgtgtc ccggcaaagt cggccgtttt
     7321 catcatatag ccttcattca gcagccagtt cccggtgcct atacctctta atatcaattc
     7381 attgccatct ccatcgtgaa tgtatttacc ggaagtatgg aggtaaccct gtgcaaagga
     7441 atgattcaat gctgaaataa ggagtgaaaa aaagaaaatc tttagaatgg ataaattcat
     7501 ctgatcgtat ttctgaaata tatcttttgt aaaggtaata ccgttatttt ctccggatgg
     7561 aaataattat gcatttgaac gtgggaaata ggcatggaaa gtacttcctt taccgggttg
     7621 actatcggcc agtataaagc cgtggttctg gtcgacaatg ccgtaaactg tcgataaacc
     7681 caggcctgtc cctttaccga cgggtttggt ggtaaaaaaa ggatcaaaaa tttgctgaag
     7741 tatctctttt tccattccgc atccattatc ccggatggtc agcctgacaa attcaccggg
     7801 cctgcttccg tgcaattttt tggatgcggc caccgtaatc ttctcattcc cggtgatcac
     7861 attgatggtt ccgttgtcgc cgatggcatc cctggcattg atggtaagat tgatcaggac
     7921 ctggtttagc tgtaccgggt cgattcgcgt ctgccagagg tcaatgccgg gtatccagtt
     7981 aacttttatc cggtcgccga tcagccggtg gagcatgtcc aggatacttc cgatgtaatc
     8041 gttgagattg atgatgacgg gattgaaagg ttgtttgcgc gcaaatgtca gcaattgccc
     8101 gataaatgcc gaagattgcc tgaccgaagt cctgatctgg tccagatggc cggcaacagg
     8161 gtgatcctga tccagtttca ttattgaaag ttcggtgagc gtgttgatta cctgcaaagt
     8221 attgttgaac tcatgggcaa cgccaccggc cagtttacca atgacctcca gtttccgggc
     8281 ctggaataac tgttcttcaa ttcttctctt ctctgcttcg gttttcttaa aatttgtaat
     8341 atccagtgca gtaaaaataa cgccctgtga attatcgtga ttcaccaaag gggttgaagc
     8401 cagcaggatg tcgatcacgg tcccgttttt tcttttccag cgggtttcaa cgacgcctat
     8461 accggttttt tcgatctgcc tgtacttttc tgtgccgaca taatcgtaat cctcttttga
     8521 aggataaagt atgagcgcat tttgtccgat caattcatcc tgtgaataac cggtcatttc
     8581 gcagatacgg tcattgattt ctgtgatcat cctgttgata accattccta ttccggaggg
     8641 ggacacacgg aatacgctca tgatcttctc ctggtttctt ttcagttctt cttcaacatg
     8701 aattctttcg gtaatatcca tgataaaacc ttccagtcgc ctttccgtat tgtcatttcc
     8761 caaaacctgc cttcccttct cccataccct tctttccgtt ccgtcttttg cctttatcct
     8821 gtattccaaa gtgaaaggca tatctttaac cacgtactcc tggatggtgt cattcacata
     8881 attccggtca tcgggatgga taatatcctc atattgaagc ttctttccca tgataaagtc
     8941 ttccgggtca taacctgtca ggtccaggca gccctcactg ataaattcca tgggccatcc
     9001 gggcgcgttt ttgcaccggt atgccatccc gggcaaattg ctgagcagga cctccagttg
     9061 cttatcgctc tcccgcaaca acggattcgc ttcctggtca ccgggaaaca aatcttcttc
     9121 cagcagtaag cgcaggtctt ctatagaccg gaccgcctcc gctgtctggc cggaaatagt
     9181 attaaaaaat tcccgggctg attccggaat atcctgatca tgatacccaa gctgagcata
     9241 aagtttaatg acagccaaag tattcctgtg atcatgaaag agttttctga tttcaggagt
     9301 caactttttc ataaaaattt ccggtgtaag ttataataag gtgcttaccg gatatcaaag
     9361 ataattcaat ggagaatttt ttccaacaaa ttcgcaattt gccggatatc agccggcttt
     9421 tgcagccatc cggccagtcc tgtcccggtc atttcttccg cttccgccgg tgtcatatat
     9481 cctgaaagca tgacaaaagg aatatccagg tttcgttttt tcatttcgtt gaacaaaccc
     9541 cggccatcca ggatgggcat agaccagtcg caaaggatta acgccagttc cggctcctca
     9601 ccggtctccg gctggctgca gctcttttcg atgatttcaa gggcttgctg accgttttct
     9661 gcttcgactg ttttataatt caggaatttt agagtctctg acaacgcgga acgcatgggc
     9721 agattatcat caaccaccag aattcttttt tttgaagttt gattcatcaa ttcagactgt
     9781 tctatccgtt ccaaatttaa atgccggctt atcctcattg gctttaaatt taaacattta
     9841 tgttgaaaca aagaaaaggt tattgtatct ttcgaaacaa taacctttgg cagcaattaa
     9901 tcaacacact tcttattaga acttattatg agttaacttt aaaacatgag aaatattaaa
     9961 cccgtaacaa taaattctta aagtattcta ttattattag ttagcaaaaa tagtggtatt
    10021 gcgtgttaat aaacaacagt ataaatacct attttaatat ttaagaatac gtaggaggaa
    10081 tactaaatca gtttgttttt aagggcaaac atgatgagtt cggcggtgtt ttttgtctgg
    10141 gttttggcga ggatattttc cctgtgttta tcaactgttc ttttgctgat attcagctcg
    10201 gcagcgatct cgttattgga tttgcccgaa gaaactcttt gcaggatttc ccgttcccgt
    10261 tcggtaatca ggtcggtttg cgacttttcc ttattcattt tcctgaccag gctatacatc
    10321 agatcctggg agaagacttt attcccgttg gctaccgatt cgatggctgc cgatacatct
    10381 tccagggcag aatctttcga taaaaaaccg acaacccctg cttcgatcat atcctggaag
    10441 tgctcgatat cgttatgcat cgataaagca ataattttca gttgattatt cccggatacg
    10501 gcaatcctgg tcgcttctat cccatccaat accggcatac tgatatccat caggacaaca
    10561 tcgggtgttt gggtcttgat cagctcgaga aactcctctc cattggaagc ttcaaagatc
    10621 cggttgataa tttttgcgga ggagagcgca gctgaaagac cctggcgaaa cagcgtgtga
    10681 tcatccacaa tggcgatgtc aagttttttc ataaaaatgg gatcaatcaa atataagaat
    10741 aataatgttc aatatcattc agtatctgat taattttttc tgatgacgat tcttccatca
    10801 ataatacttt gtatcgttta aaatcaggaa agcctttgaa ataatcactg taatgttttc
    10861 ggatttcatt gacagccacc ctttcccctt tccattccac cgatttttcc aggtgaatac
    10921 ggcagattct aaccctttca tgaatatcgg gagcggagag gatccggccg gtggccaggt
    10981 agtgtttgat atccctgaat atccatggat ttccgtaact tccgcgtgca accatcagcc
    11041 cgtcgacatg atagttctct ttgaagaact ttgccgcctg gggtgtaaag acatcaccgt
    11101 tgccaaaaac agggatatgc atggccgggt tttccttgac tttaccaatc agtgtccagt
    11161 cggccggttc gctggaacgg gttacccgtg tgcgcccatg aatagtaatc gcctgaatgc
    11221 caacgtcctg tagcctctcg gcaatttcca cgatctcctt ccggtcgttg tcgtaaccca
    11281 gacgcgtttt aaccgtaacc ggtttgttaa cggccctgac caccgcttcg gtcatcttca
    11341 ccattttggg gatatcattc agaatccctg atccggcccc tttcccggca acttttttta
    11401 ccgggcagcc gtaattgata tcgatgatgt cgggattggc ttcttcggca taccgggccg
    11461 ctttcaccat cgattcgata tcatgtccga agatctgtat cccgatcggc cgttcgaatt
    11521 cggtgaattc cagtttttta agactttttg ctgcatcccg gatcaacccc tcggaagaga
    11581 taaactcggt ataggtaata tcggcgccga acaatttgca tacataccgg aaaggcggat
    11641 cactgacatc ttccatgggc gccagcaata tagggaattc gcccgcatct ataggtccga
    11701 tctttaccat tgcatcgttg tcgaggagac tgcaaattta ctatttttac ggccataacc
    11761 ggagttatgt tcagggaacc gccattcagg gatttctcgc cgtttctgaa gat
//
LOCUS       DAOPNH010000071        11750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5763_length_11750_cov_7.031210, whole genome shotgun sequence.
ACCESSION   DAOPNH010000071 DAOPNH010000000
VERSION     DAOPNH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_11750_cov_7.031210"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="PKZ74_05325"
     CDS             complement(<1..1604)
                     /locus_tag="PKZ74_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92212.1"
                     /translation="MKRFLLLLLAIGGFTVAFSQTGTIKGFVFDKSNGEPVIFTNVYL
                     LKTTHGSATDENGYFIITKVPAGDYTLIVTYLGYDTLTMPISLQSGGVITKQLYLNPA
                     AINLETINISAEYQEARTETKTSVVKITPKDIGLIPSIGGQPDLAQYLQVMPGVVFTG
                     DQGGELYIRGGSPIQNKVLLDGVTIYKAFHSIGLFSVFETDIIRSADIYTGGFGAEYG
                     GRISSVMDITTRDGNRTRFAGKVGLSSFGANLLLEGPFVKIKENRSGSAGFILSAKGS
                     YLEQSSELLYKYIDTAGLPFNFLDLYGKVSIQASNGSKINFYGFHYRDQVNEYKTLSD
                     FNWHSTGIGTNFLVIPGKSDFIMEGHVAYSNYTIGLIEETSPERTSRISGFDVGLDFT
                     YFLGKDELKFGLEQVGSTTDYYFVNGSGMVIEQQENTTEIGLFAKYKLTAGKFLIEPS
                     FRLQWYASLSTVSPEPRLALKYNVTKHFRLKFAGGLYSQNLISARSDRDVVNLFYAIL
                     SGPDNLPDYFDGKPVTHALQKATHLIL"
     gene            complement(1704..2624)
                     /locus_tag="PKZ74_05330"
     CDS             complement(1704..2624)
                     /locus_tag="PKZ74_05330"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPM92213.1"
                     /translation="MKAKNIIWGLVLVLIGVMFILKNLDVIYFSWHSIWRLWPIVLVL
                     IGVTILPIKDLYKIILTVIVMIIGALFLIYVPGNRDNDWSFRFDRDITPPSPPDKLKD
                     IDQRIFEAYDTIITSAKLDFDAAAGNFRIEDTTAELFEFEREGNLGKYVYSIKDLGSR
                     REINIELDEGRIRGDFRNKVAVKLNPLPVWDLKVDVGAANIDMDLSSFKVENIDIDGG
                     ASSINVKMGSLYDNVNVKINSGASSINIKVPEEFGCKIVANTVLSSKDLNGFNHTGGG
                     NYETPDYQDKSKFITIKIEAAVSSLFIQRY"
     gene            complement(2628..3095)
                     /locus_tag="PKZ74_05335"
     CDS             complement(2628..3095)
                     /locus_tag="PKZ74_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPM92214.1"
                     /translation="MQRKGLYRSRRDRILGGVAGGIAQSLNADPAIIRLIFAVLVIVG
                     GGGLLLYLILWIAIPEEPFEYYQSAFNPETSPGGQPAEGGETAAPSPVVYPARRNNGG
                     LIVGLVLICLGALFLLDRFLPTIHLHFHDFWPIIIVVAGITLIVSSFAGRKKS"
     gene            complement(3225..4031)
                     /locus_tag="PKZ74_05340"
     CDS             complement(3225..4031)
                     /locus_tag="PKZ74_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPM92215.1"
                     /translation="MTKESAIKKGPEQAEQLSDEGIKRKIEDTLLALYQLQQHDSQID
                     KIRMIRGELPLEVQDLEDEIIGLQTRIENYHDEIKSFDKLIAEKKQAIKDSESLIRKY
                     EEQKMNVRNNREYDSITKEIEFQTLEMQLAEKRIKEYNEALNGKNAQISEAEGVLNER
                     RSDLEIKKSELNDIVEETENEEKTLAQRSEELQGRIDERLLMAYQRIRKNARNGLAVV
                     QIERDACGGCFNKIPPQHQLDIRMHKKIIVCEYCGRILVDSDITGMVKKN"
     gene            complement(4043..5137)
                     /locus_tag="PKZ74_05345"
     CDS             complement(4043..5137)
                     /locus_tag="PKZ74_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPM92216.1"
                     /translation="MVLKELLAAIEEMAPFSLQEDYDNSGLQIGSSGQEVTGCIICLD
                     INEQVVEEAARHGSNLIISHHPLIFKGIKRIADKTATERIIKKALKEDLAICSVHTNL
                     DNAWNGVNRILGEKLGLTGLSVLRKPSGMLKKLATYCPAAHAGRVRQALFDAGAGHIG
                     GYDQCSFNLTGQGTFRGGEATNPFVGEKGHLHFEEEVRIETIFPAYRKESVVEALLCA
                     HPYEEVAYDIYSLENEYDRAGAGMIGHLPEPTDEKVFLAQVKDILKIPVIRHSPLLGK
                     PLRRVAICGGSGSFLIQDAIRMQADIFLTADIKYHQFFEADGKIVIADAGHFETEQFT
                     CGLIAAYLKKKFPTFAIRISETLANPVNYF"
     gene            5261..6328
                     /gene="lpxK"
                     /locus_tag="PKZ74_05350"
     CDS             5261..6328
                     /gene="lpxK"
                     /locus_tag="PKZ74_05350"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPM92217.1"
                     /translation="MIIVKILLFPFACLFGAVIGLRNKLFDWKIIPARSFHFPVIAVG
                     NLSAGGTGKTPHIEHLIRLLKNHYKVAVLSRGYRRKTKGFILCRPEHSQLEIGDEPAQ
                     YLNKFPDITLAVDEDRVRGIKRIMDERPETEVILLDDAFQHRYVKPGRSILLTDYHHL
                     YTEDYLLPVGLLREKSKGAKRADFIIVTKTPKVLSPITRRNLVKEIAPRPHQKLFYSY
                     VSYDRPIPLKRCQNQKPAAEKYSYIIMVAGVANSYPLQEYLKTICNELIIFDFHDHHQ
                     YSRSDFEKILNEYHSIISKDKVIFTTEKDATRLDTDEFSAYLDVLPVYYIPIRINFHD
                     CDEIKFDKLILDYVRKSIGSR"
     gene            6300..7127
                     /locus_tag="PKZ74_05355"
     CDS             6300..7127
                     /locus_tag="PKZ74_05355"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPM92218.1"
                     /translation="MFEKVSEAVDYIRTRTGLIPETGIVLGTGLGNLANEIESPVFIP
                     YDEIPHFPSSTVEGHAGRLIIGGLNGCRVVAMQGRFHFYEGYDLQDVVFPIRVMKFLG
                     INLLILSNASGGLNPGFQVGDIMIVEDHINLMNLMNENPLKGVHDERFGARFPDMSRP
                     YDAGLIKKAEILAEKMGIHYRKGIYAGVTGPTFETPAEYSYIRYLGADAVGMSTVPEA
                     IAAAQMQLPVFAVSVIADLGVKGKIVEISHKEVIDAASLAEPLMTRLIKALLGELFP"
     gene            complement(7131..7616)
                     /locus_tag="PKZ74_05360"
     CDS             complement(7131..7616)
                     /locus_tag="PKZ74_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92219.1"
                     /translation="MKKSVILWILLLILVRCHGQVFLLVENDKTLKNFKYYPGDVLSF
                     SLGPSQGRITDQITGMTDSTLIFEYTGEVGIDRLVTIYRSNWLMMTLSSLSMLAGAGY
                     IGLDSFNRLINREYPVIDAGTALVSAALIGGSFALMPFHDRRLKCHTRWKLKVLDQSS
                     F"
     gene            7703..8887
                     /locus_tag="PKZ74_05365"
     CDS             7703..8887
                     /locus_tag="PKZ74_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM92220.1"
                     /translation="MMAVRNIFIFRVLLLALIVLAAAGCQRDGKKSIKTTITGSFSQL
                     AGKSVSLSEFDITSATPLDTVKIDDKGNFRFKFRRKGAGFYLVKVDNRNFITLVLDQE
                     KNIRIQSSEANIRKNYSINGSPDSEIFRDFEMFTEVNRNKVDSLSKTFNNQMRSTAFR
                     SLKPELDKEYQEIFGQQVRYAQQFIENHCSSLSTLLVLNKRFGERRILTEEDNFTHFV
                     MADSCLRFHYPDNKHLKELNKKITIARQNRRIFDITEAQLAPGKTIPDISLGDVNGKQ
                     VRLYSFEGRPVILYFWASWDHSSRKANKVIRELAENSAQNPVVYAIALESYKEMWQEA
                     IRQDGLDNWINVSDLLNIKSSAKTMFNIPDTFPYFFYLDKDLYIRYKGNDFSRLETEL
                     NR"
     gene            8953..9732
                     /locus_tag="PKZ74_05370"
     CDS             8953..9732
                     /locus_tag="PKZ74_05370"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPM92221.1"
                     /translation="MEDNVSRSSGKIYFASDFHLGIPDHASSRQREEMLVSWLDMIQS
                     DASDIFLMGDVFDFWFEYKTVVPKGYIRLLGKLAQLTDQGLKVHLFTGNHDIWAFSYL
                     QDEAGIELHRKPEVRDMMGKTFYLAHGDGLGPGDNGYKFLKKVFSCRFNQYLFRWLHP
                     DIGARMGLYFSGKSRLANMIKEDKAENLNRIEDEMLYQYATGILQNGTKPDYFIFGHR
                     HRPENIELPGGSRLIILGDWLTHFTYAEFDGKQIHLKKFKS"
     gene            9736..11505
                     /gene="sppA"
                     /locus_tag="PKZ74_05375"
     CDS             9736..11505
                     /gene="sppA"
                     /locus_tag="PKZ74_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPM92222.1"
                     /translation="MKDFLKFMLASMLGFVIILTLSFFILIGIFASIASMADKKSVSI
                     ESSTVLQIKLENEVFDRAPKDPMTFFSPAGFTMEQEPGLDEILKNIKKAQTDDKIKGI
                     FLDLSVVPSGLATVREIRNALIEFKSGGKFIIAYGEVYSQMAYYLGSVADKMYLHPAG
                     AIDFRGFGAQVFFLKGTLEKLDIEMQVIRHGKYKSAVEIFTEDKMSEASREQMNAMVT
                     DFWATALADISASRNISLARLNEIADNLDAYEAGKALNIGMIDGLYYRDQLLDELRNK
                     LGLGEKEKVRTVSLAKYTLVTPATPQKVSANKIAVVYALGNIIDGKGDDRTVGSVNIS
                     EAIRKARENDKVKAIVLRVNSPGGSASASEVIRREVVLAAKEKPVVVSMGDVAASGGY
                     WISASASYVMADPATLTGSIGVFGMIPNMQGLFNNKLGITFDEVNTNKYSDFPNVSRP
                     MSDYEILVIERQIEKIYDDFLSLVAEGRKMEKSQVDEIGQGRVWSGSDAKEIGLIDEF
                     GGLTAAIDKAAGLAGITEYNLMSLPAQKEPLQQIIDELTGNVAAGRIEKELGEYYTYY
                     DYIKQIKEASGIQARLPFEVKIK"
     gene            complement(11517..>11750)
                     /locus_tag="PKZ74_05380"
     CDS             complement(11517..>11750)
                     /locus_tag="PKZ74_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92223.1"
                     /translation="YRYNPLLEAEGKNPFQLDSKEPDWSQFQGFLSGEVRYASLKKAF
                     PSEAARLDALAEQNARWRYTTYKRLAGMDFSEK"
BASE COUNT         3176 a         2885 c         2675 g         3014 t
ORIGIN      
        1 aggatgagat gggtggcttt ttgcagggca tgggtcaccg gttttccgtc gaaataatcg
       61 ggaaggttat cgggaccgga aaggatggca tagaaaaggt ttacgacatc cctgtcagac
      121 cgcgcactga tcaggttctg cgaatacagg cccccggcga atttgagcct gaaatgtttg
      181 gtcacattat atttcagcgc caggcgcggc tccggcgaaa ccgtcgacag cgaggcatac
      241 cattgaagcc ggaaactggg ttctatgagg aatttgcccg ccgtcagctt gtacttggca
      301 aacaatccta tttcagtggt gttctcctgt tgttcaatta ccatccccga tccgttcaca
      361 aagtaataat ctgtagtgga acccacctgt tccagcccga acttgagttc atcctttcca
      421 aggaaatagg taaaatcgag gccgacatca aacccggaga tcctgctggt ccgctccggg
      481 ctggtctctt caatcaggcc gatggtgtaa ttggaatagg ctacatgccc ctccatgata
      541 aaatcagact tgcccgggat caccaggaaa ttggtgccga ttcctgtgct gtgccagttg
      601 aaatcagaaa gggtcttgta ttcattcacc tggtcacggt aatgaaaccc gtaaaaattg
      661 attttgctgc cgttgctcgc ctggatcgaa actttaccgt aaagatccag gaagttgaaa
      721 ggcaatccgg ccgtatcgat atacttgtat aataattcgg agctttgctc caggtaagaa
      781 cctttggccg ataaaataaa acctgcgctc ccgctcctat tctctttgat tttaacaaac
      841 ggcccctcca gaaggaggtt cgcgccgaaa gaacttaaac ccacctttcc ggcaaagcgc
      901 gtgcggtttc catccctcgt cgtaatgtcc atgaccgaag agatccgtcc gccgtattct
      961 gcgccgaaac ctccagtgta aatgtcggca ctgcggatga tatcggtctc aaaaacactg
     1021 aacaggccaa tagagtgaaa tgctttgtaa atagtgaccc cgtcgagcaa tactttgttc
     1081 tggatcgggg aaccgccccg gatatacaat tcaccgccct ggtcacccgt aaaaacaacc
     1141 cccggcatca cctgcaggta ctgggccagg tcaggctgcc cgccaatgga tggaatcaac
     1201 ccgatgtcct ttggtgtaat tttaaccacc gaagtttttg tctcagtccg ggcttcctgg
     1261 tattcggctg aaatattgat ggtttcaagg ttaatggctg cagggttcaa atagagctgc
     1321 tttgtaatga ccccgcccga ttgaaggctg atcggcatcg tcagggtatc atagcccagg
     1381 taggtgacaa tcagggtgta atctcctgca ggcactttgg taattataaa ataaccgttt
     1441 tcatctgtcg cagatccatg agtggttttt aaaaggtaaa cattggtgaa gatgaccggc
     1501 tcaccgtttg atttgtcaaa aacaaaccct ttgatggttc cggtctgact gaacgctaca
     1561 gtaaaaccgc caatggccag taagagaaga agaaaccttt tcatcagact ctgggttgaa
     1621 aatttgcaaa aacggcaaat ttaccattat tcccaaatgc cgggctgaaa aatcagtgaa
     1681 cggtagaatt taacaagcct gtctcagtat cgttgaataa acaggcttga aacggctgcc
     1741 tcgattttaa tcgtgataaa tttgcttttg tcctgataat cgggtgtctc gtagttgccg
     1801 cccccggtat ggttgaaacc attgaggtct tttgaagata gaaccgtatt ggccactatc
     1861 ttgcagccaa actcttccgg aactttaata ttgatcgaag aggcacccga atttatcttg
     1921 acattcacat tatcgtaaag cgaacccatc ttcacattga tcgacgacgc cccgccgtca
     1981 atatcgatat tttccacctt gaacgacgaa agatccatat cgatattggc tgcccccaca
     2041 tcgactttca ggtcccaaac aggcaacgga ttgagcttaa ctgcgacctt gtttctaaaa
     2101 tcccccctga tccggccttc atccagctca atgttgatct cccggcggct gccaaggtct
     2161 ttaatggaat aaacatattt acccaggttt ccttcccgct cgaattcaaa caactcggca
     2221 gtagtatcct caatacggaa atttccggcg gccgcatcga aatccaattt tgccgaggtt
     2281 atgatcgtat catacgcttc aaagatccgc tgatcaatgt cctttaactt gtcaggcggg
     2341 ctgggagggg ttatatcccg gtcaaaccgg aacgaccagt cgttatcccg gttacccgga
     2401 acatagatca ggaacaatgc ccctatgatc atgacgatca cggtgagtat aattttataa
     2461 agatctttga tcggaagtat ggttacgcca ataagcacaa gaacaatggg ccagaggcgc
     2521 cagatgctgt gccagctaaa ataaatcaca tcgagattct tcagtataaa catgacccct
     2581 atcagaacca ggaccagtcc ccagataata ttctttgctt tcatggatca ggattttttt
     2641 cttccggcaa aactactgac aataagtgtg ataccggcca ctacgatgat gatgggccag
     2701 aaatcatgaa aatgaaggtg aattgtaggt aaaaacctgt cgagaaggaa cagggcaccg
     2761 aggcagatca gcaccaggcc gacgataagg ccgccgttgt tccggcgcgc aggataaacc
     2821 accggcgaag gtgcagcagt ttcacctcct tcagcaggct ggccaccggg tgaggtttcc
     2881 ggattaaatg cggattgata atactcgaaa ggttcttcgg gaatcgcgat ccagaggatc
     2941 aggtagagca gcagcccgcc ccctccgaca attaccagga cggcaaaaat gagcctgata
     3001 atggcaggat ctgcattcag cgattgggca atgccgccgg ctacaccacc cagaatacgg
     3061 tcacggcggc tgcggtacaa tccttttctt tgcatgtgat ttaaatttgt tataaatgta
     3121 taaacctaag acgaggtttt caatttcatg ttacaaatat aacaaaataa aaagggtgtc
     3181 tcaaatgtca gagcaccctc tttataaaca gaatttcaat aagctcagtt cttttttacc
     3241 atgccggtaa tatccgaatc gaccaggatc cggccgcaat attcgcatac aatgattttc
     3301 ttgtgcatcc ggatatccag ctgatgctgg ggaggaatct tgttgaaaca cccaccgcag
     3361 gcatcccgct cgatctggac cacggcaagc ccgttgcggg cattcttccg gatccgctgg
     3421 taagccatca gtaacctctc gtcaatcctt ccctgcaatt cctccgatct ctgtgccagc
     3481 gtcttttctt cattctccgt ctcctccaca atgtcgttca gctcgctctt ttttatctcc
     3541 agatcagaac gcctctcgtt caatacacct tccgcctcgc ttatctgtgc gttttttccg
     3601 ttcagggctt cattgtattc tttgatcctc ttctcagcca gttgcatctc aagcgtttga
     3661 aactcaattt cttttgtgat cgaatcgtat tccctgttgt tccttacatt catcttttgc
     3721 tcttcatatt tgcggatcag cgattcactg tctttgatgg cctgcttttt ctcggcaatc
     3781 aacttatcaa acgattttat ctcgtcatga taattttcga tccgggtctg taaaccgatg
     3841 atttcatcct ccaggtcctg cacttccaga ggcagttccc cacggatcat ccgaatttta
     3901 tcgatctgcg aatcatgctg ctgaagctgg tataacgcta atagcgtatc ttcgattttt
     3961 cttttgatcc cttcatctga aagctgttcg gcctgttcag gacctttctt aatcgcactt
     4021 tcctttgtca taaattattg atttaaaaat aattaaccgg attagccagg gtttctgaaa
     4081 ttcggattgc aaaggtagga aattttttct tcaaataagc agcgattaac ccgcaagtaa
     4141 attgttcagt ttcaaaatgt cctgcatcag caatgacgat tttcccgtcg gcttcaaaaa
     4201 actggtggta ttttatgtcg gccgttagaa aaatatcggc ctgcatccgg atggcatcct
     4261 ggatcagaaa actcccggag ccgccacaaa tagccacacg cctcaaaggc tttcccaata
     4321 aaggagaatg acgtatcacc ggaatcttca ggatatcttt cacctgtgcc agaaaaacct
     4381 tttcatccgt aggttcaggc aaatggccga tcatccctgc acctgcccgg tcatactcgt
     4441 tttccaggct gtaaatgtcg taggctactt cttcataggg atgggcgcat aaaagcgctt
     4501 caacaaccga ttctttccgg taggcaggga agatggtctc tatccttacc tcttcctcga
     4561 aatggagatg ccctttctcg ccgacaaatg ggtttgtcgc ttcccctccg cggaaggtcc
     4621 cctggcctgt gagattgaaa ctgcactggt cgtacccgcc aatatgaccc gccccggcgt
     4681 caaacaaagc ctgcctcacc cttcctgcat gcgctgcagg gcaataggtt gccagctttt
     4741 tcagcatccc tgagggttta cgcaacactg aaagcccagt aagaccgagc ttctcgccca
     4801 gaatccggtt cactccgttc caggcattgt cgaggttggt atgaaccgag cagatggcca
     4861 gatcctcttt gagtgctttt ttaatgatcc tttccgtcgc cgtcttatca gcaattcttt
     4921 ttattccttt gaatattaaa ggatgatgac tgatgatcaa atttgagccg tgcctggcgg
     4981 cctcttccac aacttgttca tttatatcaa gacaaataat acaaccggtc acttcctggc
     5041 cagaagatcc tatctgtaaa cccgaattat cataatcttc ctgcaatgaa aacggcgcca
     5101 tctcctcaat agctgcaagc aattctttta aaaccatatc aatatatttt ttttatagaa
     5161 aaagtgactt ttttaaattc catccgtata aatataaaac tttaatgttt tcttaatctg
     5221 caatataact tctgaaacag attgtcggta tatttaacag atgattattg taaaaatact
     5281 actatttcca ttcgcctgcc tctttggggc tgttattggt ctgagaaaca aattgtttga
     5341 ctggaaaatc attccggcca ggagtttcca ttttcccgtc atcgcagtcg gaaacctgag
     5401 cgcaggagga acgggaaaaa cccctcatat cgaacacctc atccggttgc tgaaaaacca
     5461 ttacaaggtt gccgtcctga gccggggata ccgcagaaaa accaaaggat ttatcctctg
     5521 ccgaccggaa cattcacagc ttgagatcgg cgatgaaccg gctcaatatt tgaataaatt
     5581 tccggatatt accctcgccg tcgatgaaga ccgggtcagg ggcatcaaac ggatcatgga
     5641 tgaaagacct gaaacagaag tcattctcct ggatgacgca ttccagcacc gctatgtcaa
     5701 acccggaaga tccatcctcc tgaccgatta ccatcacctg tacacagaag attatctgct
     5761 tccagtcggg ctgctcaggg aaaaatccaa aggcgccaaa agggccgact tcattatagt
     5821 taccaaaacc cccaaggtgc tctcccctat cacccggcgc aatcttgtga aagagattgc
     5881 cccccgtccc catcagaaac tcttttattc ttatgtttca tacgacaggc cgataccgtt
     5941 gaaaagatgc cagaaccaga aaccggccgc cgaaaaatac agctacatca tcatggtggc
     6001 cggagtggct aattcttatc ctttgcagga atacctgaag acgatttgta acgaattgat
     6061 aatatttgac tttcatgatc atcaccaata ttcccggagt gattttgaaa aaatattgaa
     6121 tgaataccac agcataatct caaaagataa agttatcttc accaccgaaa aagacgcgac
     6181 acgcctggat actgatgaat tctccgcata tctcgatgtg ttgccggttt attacatccc
     6241 catcaggatc aatttccatg attgtgatga aataaaattt gataaactta ttctggatta
     6301 tgttcgaaaa agtatcggaa gccgttgatt atatcagaac caggaccggg ctcatcccgg
     6361 aaaccggtat cgttctcggt accggactcg gaaacctggc caatgaaatt gagtcacctg
     6421 tttttattcc ttacgacgaa atcccccact ttccctcttc caccgtcgaa ggccatgccg
     6481 gccggctcat catcggcggg ctgaacggat gccgcgttgt ggcaatgcag ggcaggtttc
     6541 acttttacga aggttatgac cttcaggatg tcgtttttcc catccgggtg atgaagtttc
     6601 tcggaattaa tttactgatc ctttccaatg ccagcggcgg actaaatccc ggttttcagg
     6661 tcggtgatat tatgattgtg gaagatcata tcaacctgat gaacctgatg aatgagaacc
     6721 ccctcaaagg agttcacgac gagcgcttcg gagcgcgttt cccggatatg agccggccat
     6781 acgatgccgg attgatcaaa aaagccgaaa tactggccga aaagatggga atccattacc
     6841 ggaaagggat ttatgcggga gtaaccggcc ctacctttga aacacccgca gaatacagct
     6901 atatcaggta cctgggagcc gatgccgttg gcatgtcgac agtccctgaa gcgatcgccg
     6961 ccgcccagat gcagctgccg gtctttgccg tatctgttat tgccgacctg ggggtcaaag
     7021 gaaaaatcgt cgaaatctcc cacaaggagg tcattgatgc cgccagcctg gccgaacctc
     7081 taatgacccg gctgattaaa gcgttgctcg gtgaattgtt cccgtaattc ttaaaatgaa
     7141 ctttgatcca gcaccttgag cttccagcgg gtatggcatt tcaatcgcct gtcatgaaat
     7201 ggcatcaggg caaaggatcc accgatcagc gcagcggaaa ccagcgcagt accggcatcg
     7261 atcaccggat actcgcggtt gatcaggcga ttgaaactgt cgagaccaat ataaccggcg
     7321 ccggccagca tgcttaagct gctcagggtc atcatcagcc agttcgaccg gtaaatagtc
     7381 accaggcggt caattccgac ttctccggta tactcaaaga tgagagtaga atcagtcatc
     7441 ccggtgattt gatctgtgat cctcccttgc gatgggccaa ggctgaaact caatacgtcg
     7501 ccgggatagt atttgaagtt tttaagcgtc ttatcattct caaccagaag aaatacctgg
     7561 ccgtgacatc tgacaagaat cagcaataat atccagagga tgacgctttt cttcaaatta
     7621 cccggtgaaa tgacgattta aacaataaac tcaggaacga cctttatcgt atttttgtct
     7681 aaaataataa aaatcgggcc tcatgatggc agttagaaat attttcattt tccgggttct
     7741 tttgctcgcc ctgattgtat tagctgcagc aggatgccaa agggatggta aaaaaagtat
     7801 caaaaccaca atcaccggat ccttttcaca actcgcagga aaatcagtgt ctctcagcga
     7861 gtttgatatt acctcggcga ctccgttgga tactgtgaaa attgacgata aaggaaattt
     7921 ccgctttaag tttcgacgga aaggcgccgg attttatctg gtcaaggtgg ataaccggaa
     7981 cttcattact cttgtgctcg accaggagaa aaacatccgg attcaatcat cggaggccaa
     8041 catccggaaa aactattcca tcaatggatc gcccgattcc gaaatattcc gcgattttga
     8101 aatgttcact gaagtaaacc gtaataaggt cgattcgctg agcaaaacat tcaataacca
     8161 gatgcgttcg accgcttttc gctctctgaa accggaactg gataaagaat accaggaaat
     8221 tttcggacaa caggtgcgct acgcacagca gttcattgag aatcactgtt cttcgctgtc
     8281 cactttgctg gtgttgaaca agcgttttgg agaaagaaga attctgacgg aagaagataa
     8341 tttcactcat tttgtgatgg cagattcttg cctgagattt cattatcctg ataataaaca
     8401 ccttaaggaa ctgaataaaa agattacaat tgccaggcaa aaccgccgca ttttcgatat
     8461 taccgaagca cagctggcac ccggcaaaac gatccctgat atctcattag gcgatgtgaa
     8521 tggaaaacag gtcaggctgt attcctttga aggcaggccc gtcatcctct atttctgggc
     8581 atcctgggac cattcgagcc ggaaagccaa taaagtcatt agggaactgg ccgaaaattc
     8641 ggctcaaaat ccggtggtat atgccattgc gctggaaagc tacaaagaga tgtggcagga
     8701 agctatccgg caggacgggc tcgataactg gatcaatgtt tctgatctcc tgaacattaa
     8761 atcatcggca aaaacaatgt tcaacatacc cgatacgttt ccttatttct tttacctgga
     8821 taaagatctg tatatccgtt acaaaggaaa tgatttcagc aggctggaaa ctgaattgaa
     8881 tcgttaagtt gccacgccgc aattggatta taaggggaag tttagtaata ttgcgttaaa
     8941 atgatcaata ttttggaaga taacgtttcc cggtcatccg gaaaaatcta cttcgcttcc
     9001 gattttcacc tgggcatccc cgaccatgca tcgagccgac aaagggaaga gatgctggtt
     9061 tcctggctcg atatgatcca atccgatgct tcggatattt ttctcatggg tgatgttttt
     9121 gatttctggt ttgaatacaa gaccgttgtc ccaaaaggat atataaggtt attggggaaa
     9181 ttggcccaac tgaccgacca gggtctgaaa gtgcaccttt ttaccggtaa ccacgacatc
     9241 tgggctttca gctatttgca ggatgaagca ggaatagaac ttcaccgcaa gccggaagtc
     9301 agggacatga tgggcaaaac cttttacctg gctcatggcg atggccttgg cccgggcgat
     9361 aacgggtaca aattcctcaa aaaggtattc agttgccggt ttaatcagta tctcttccgc
     9421 tggctgcatc ccgatatagg cgcccggatg ggactctatt tctcgggaaa gagcagactg
     9481 gccaacatga tcaaagaaga taaagccgaa aatctgaaca ggattgaaga tgaaatgtta
     9541 tatcaatacg caaccggtat tttgcaaaat ggaaccaaac ccgactattt catcttcggg
     9601 cacaggcaca ggccggaaaa tattgagctt ccgggcggat cccggctgat catcctgggc
     9661 gactggctga cccattttac ttatgccgaa tttgacggaa aacaaatcca tttaaaaaaa
     9721 ttcaaatcct aggttatgaa agatttcctg aaatttatgc tggcctcgat gttaggcttt
     9781 gtcatcatcc tgacattatc attctttatc ctcatcggga tcttcgcttc catagcgtct
     9841 atggccgata agaaatcggt ttccatcgag tcctcaaccg ttctgcagat aaaactggaa
     9901 aacgaagttt ttgaccgggc tcccaaagac ccgatgacct tcttttcccc ggccggattc
     9961 accatggaac aggaacccgg gctcgatgaa atcctcaaaa acattaaaaa ggcccagacc
    10021 gatgataaaa tcaaaggcat ctttcttgac ctgagcgttg tgccgtcagg attggccacc
    10081 gttcgggaaa tccggaacgc cctgatcgaa tttaaatccg gcggtaaatt cataatcgct
    10141 tacggagagg tctattctca aatggcctat tacctgggga gcgtggccga taaaatgtac
    10201 ctgcacccgg ccggcgcgat tgatttcagg ggatttggag cccaggtttt cttcctgaag
    10261 ggcaccctgg aaaaactgga tatcgagatg caggtcatcc ggcacggaaa atataaaagc
    10321 gccgtggaga tcttcacgga agataagatg agcgaagcca gccgcgagca aatgaatgcc
    10381 atggtaaccg acttctgggc aacagctctt gccgatatct ctgcttccag gaatatcagt
    10441 ctggcccgcc tgaatgaaat tgccgacaac ctggatgcct atgaagccgg taaagccctg
    10501 aacatcggca tgatcgacgg tttatactac cgcgaccagc tgctggatga actgaggaat
    10561 aaactgggat tgggtgaaaa ggaaaaggtc agaaccgttt ccctggcaaa atacaccctg
    10621 gtcacgccgg cgaccccgca aaaggtctcc gcaaacaaaa tcgcagtggt gtacgcatta
    10681 ggcaatatta tcgatggcaa aggcgacgac cggacagtcg gatcggtcaa tatttctgaa
    10741 gcgatccgga aagcccgcga gaacgacaaa gtcaaagcga tcgtgctcag ggtcaactcc
    10801 cccggaggca gcgccagcgc atcggaagtg attcgccgtg aggtcgtgct ggctgcaaaa
    10861 gaaaaacccg tggtcgtttc catgggtgat gtggccgcct cgggaggcta ctggatttcg
    10921 gcttccgcca gttatgtcat ggccgatcct gccaccctca ccggctctat cggtgtcttt
    10981 ggcatgatcc ccaatatgca gggattgttc aacaataaac tcgggatcac cttcgacgag
    11041 gtcaatacaa acaaatactc agactttccg aatgtgagcc ggccgatgtc agattatgaa
    11101 atactggtga tcgaaaggca aattgaaaaa atttacgatg atttcctgtc gctggtagcc
    11161 gaagggagaa agatggaaaa gtcgcaggtg gatgagatcg gccagggccg tgtatggagc
    11221 ggatccgatg caaaagagat cgggctgatc gacgaattcg gaggtttgac cgccgccatc
    11281 gacaaagccg ccgggctggc cgggattacc gaatacaacc tgatgtcgct cccggcccag
    11341 aaagaaccgt tacagcagat catcgacgag ctgacgggta acgtcgcagc cggaagaatt
    11401 gaaaaggagc tgggtgaata ctacacctat tatgactaca tcaaacaaat aaaggaagcc
    11461 agtggcatcc aggccaggct tccttttgag gtgaagatta aataattttc agaaggctac
    11521 ttctccgaaa aatccattcc ggccagcctc ttgtaggttg tgtatctcca tctggcattc
    11581 tgttcggcca gggcatccaa acgtgcagct tcgctcggga aggctttctt gagcgaagca
    11641 taacgcacct cgccgctgag gaatccctgg aactggctcc agtcaggctc ttttgaatcg
    11701 agctggaacg ggttctttcc ctctgcttcc aggagcgggt tgtaccggta
//
LOCUS       DAOPNH010000072        11749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5764_length_11749_cov_6.268941, whole genome shotgun sequence.
ACCESSION   DAOPNH010000072 DAOPNH010000000
VERSION     DAOPNH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5764_length_11749_cov_6.268941"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="PKZ74_05385"
     CDS             <1..1920
                     /locus_tag="PKZ74_05385"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92224.1"
                     /translation="LHFLTGKGKAWLNKARILFDLGNFPEALKNNEAALETYLGTDDK
                     LGLANAYNNIGLTNIYLGNYEEAMKNCDSALEFYTESGSKKGQANAYDNMGNIYQYQH
                     LWEKAAEMYFMSLKINEEIGDKNGMAGRYINLGNVYDAMGNLPEALNHNIAALNTYKE
                     LGNEYNLAIVYNNIGTLYSKQKKYPESLQMFLESLQISKAIEDTVGMIDQYINIGWLH
                     LLEKKYAESEKYYQTALELSETIGYSKESTSRSHSNLGKLYMDQGRYENAETHFLAEL
                     EISKETEDLQGTAIAYNHLGKLYVLSGDFQKAARFLNESLAISRKLDLRKGILNNLKD
                     LYQLDSAQGHFAKALENFKLYTVYSDSLLGEETNQQINRLKIEYETEKKDKEIELLNK
                     GNEIKALQLSKQKAIRHGLSAGFVLLFITGFLLFRSFRLRKKLEQQQAIINERKRISA
                     DLHDDVGSGLSRIMLLTELVKNEAKTPEMHKEAEKIASISKELSANISEIIWALNSNN
                     DYLESLVAYIRRYAAEFFDDSSVSLKINSTGTLTGIPISGELRREIFYTVKEALHNIY
                     KHAQASEAKLGFSVKDDILTVTIHDNGIGMPETVTGRYGNGLTNMQQRMTAAGGQIRI
                     ENHQGTKIILEVPVK"
     gene            1939..2613
                     /locus_tag="PKZ74_05390"
     CDS             1939..2613
                     /locus_tag="PKZ74_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92225.1"
                     /translation="MKRRRPASYLCIIMNKENITVAIVEDTDDIREALRVLINGSAGF
                     ECVHVFASAEEALAKMPVSGIDVVLMDIGLPGISGIECMKTLQPRMPETQFMISTVYD
                     DDDYIFSALQSGATGYMLKRTSPAQILEAIRDLNSGGSPMSSEIARRVVAYMQKSKRP
                     SSAADCLTEREMEILDFLAKGYLYKEIAAELFISKETVKKHIHNIYDKLQVQTRTEAI
                     NKAFRR"
     gene            2939..9208
                     /locus_tag="PKZ74_05395"
     CDS             2939..9208
                     /locus_tag="PKZ74_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPM92226.1"
                     /translation="MKKIITSLIIAICTLWAGISSSFGQALLYGVTIEGGANNQGVLY
                     EWNTAIDTYTKKFDFGGANGTWPSSSLHLYEGKFIGMTYEGGVYDNGVIYEYDPVNEM
                     YTKKFDFDGTNGSQPLYDLVFHEGKFYGTTTWGGAFNGGVIFEWDPLMNTYTKKHDFM
                     DLGGWTSTPNGKLVMHSGKFYGTCQYGGTNGANGLGYIFEWDPVTNVFTNVFDFEEPG
                     GVVPNSLTLYQSKLYGTTQGGGNNSVGVIFSWDPANDIYTTAHNFDWNLEGGTPIHDL
                     VLIQDKFYGVAANAGPLDGGVLYEFEPVSTIYTVKHAFDLNNTSNGYYPIGGVTSSGG
                     KLYGTTYYGGPSGFDLGVFYELDPLTNVYSSKFEFTGPANGAYSGYSSLLEYFPPTSD
                     NDGDGYTFTDGDCDDNDPEINPGVSELCNGIDDDCDGDIDEGFVYSVAGTSITSSEGE
                     NFCLGTTTYLSVTGGILGTGADWVWYEGGCGNSDPLGTGETLQVTPSEGTHTYFVRAE
                     GICNTSDCFSLSVTVDKAPVFQLCPTDINTGNDAGSCFSTVYYNVTATGSPAPAFSYA
                     FAGATTGNGTGTGSGSIFNAGTTTVTVTAENYCGTETCTFDVTVNDTEGPVISDCPSG
                     ITLEAEEGQWGASVSWAPPTATDNCSVTTLASSHEPGHFFNVGSTPVTYTATDEAGNS
                     SACQFLVTVTPPDFLGSVNLSTISSEITFSDNNPAPGSQLSVNALIRNNSNIDAGSFS
                     VQFADLYTNTTYPLIPLPGLNAGQSLLVSQLILAPNVPSFVPIQVAVDVTNSFDESSE
                     FDNAAVRPFVCGDVAVPGNMDLLASAVPAAVPAGSTIQICGSASYDTDPPLADPSVAG
                     AEVIISITETGQQFTGYTDEDGNFCIAYATPNTPGEFHFTVEITDNILSGDAEGSFTL
                     SEPVNPCPTDLSLSIDLPGIGSSYTGSAIIAAGTSFSGTVLVQNNCTAITEQTTLFIA
                     PPAGTVNAYEVLVPSLGPGETYPVALPVLTMNSTGDFFLSATVDFKNIVEEDQENNNT
                     EYLTLIVKPAMPDIVVAKAELASNLACFENNTVFFSILNSGLVATGPFTSVLNFYVNG
                     ILQSSQSQIIQDLGPGGQMTRSFVFTLTAPGDLIGFELFNDPANEVAELSELNNDFDF
                     ETGFRLCLPDLEVSDCYYTDVKPADAMQSGMASITATIVNQGESTLTVPFEVAFLVGS
                     TTITETVDGPLAPQQAVQVTIESPLPVDECVSLSITADPDDIVLEKQNNNNFFSGSLC
                     YDFELTNFQCGGSMVLPQTQYFCDPGQFRIGYLNHGLYEASELEVRFEISGPGLTGWV
                     LLGTESVYAGNSCGCPVVLDFPGSFYFPQTGTYQIRMTADPEDLYPESNEGNNELTVS
                     FDVVETSDYEVLAPYIFPSKINPEINEAVSFSVSYTNNGCDGVTPIELYTLVDEDPLA
                     SVTVVPLLSGDWNTAAIPQTWSSSVPGVHVFRAIIDYSEAIAEGNEMNNEATKAIYVG
                     GAPDYHVLFVSASDENPEAGQLVTLTADVLNEGQSAGSGLLRFLRADAHGVETLISEE
                     SISLAPGITQSYSTSWPVTYPGAKIIARVVNVLPAEFDLSDNEKETQLNDLQVSIAGT
                     NALCNGEYSGEIVVTITGGAAPFLIEWSDPALFGQVVNVPAGEYLVTVLDNIGLSATA
                     SITISEPEALVLTETHIDASCDNLSDGSVNLGVSGGTSPYTIIWNNGELTEDLTGLEA
                     GTYSVLVTDANNCSATLSITLAALYEAIAWYQDTDNDGFGNANDSVISCTQPDGYVSN
                     PDDCDDNNPAINPAETEICGNNFDDNCNGQVDEGCDTECDISVNAGADVTTYFGIVSM
                     QQVVRTAQVTGGTPPFTYSWTMDRPLLCNQQNSTGDESFTGGICTDNICPSSGLPGEN
                     PNCSGSATIYATMLDTAVVCVTVTDANGCSASDCFTVMASDVRCFAGNSGNHKIKMCH
                     KTNSQNNPWVEICVDTNSINAHLAHGDYIGSCDGAKSDKIESEAEDDFSFDFNLFPNP
                     AEDRITLSFHCHNESSYWIEIMDMTGRVVAKYKENSFHGDISHQIDLTGLERGIYTVT
                     LILGGQRMVKKLIRN"
     gene            complement(9273..10520)
                     /locus_tag="PKZ74_05400"
     CDS             complement(9273..10520)
                     /locus_tag="PKZ74_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92227.1"
                     /translation="MSKPHKKIYMAAGYNTISMGTGRSEFNPKKERPGLEYYFKEAAQ
                     GTLTQVGGAKNIDECAVGNFMASRFNKQAHLGGFFPMADPELQYKPAIRVEGACASGS
                     LALVTSIKNVLAGTAEVSMSLGIEVQNTVKAIYGADILAGAGWYQKRKNGHAYFFPGQ
                     FSDRAGAYYEKYGMEYARKGMGRWFRNAIENARLCPTAQEFENKVEDLEALYNKMKPN
                     GASFVDNLNVLDCSKVSDGASAIVVCTEEGLKRCGIDKKDAVEIIAWAHKVRNITEDP
                     PDPTEMTCIKKAALAALEDAGITINDLGTIETHDCFSIAGVLATEALGFADKGKGAQF
                     VADGNTARDGRIPMNTTGGLIGWGHPTGGTGVHMAVTIWEQLTGRAGAAQIKIHPEKP
                     YGMSINMGGDDGTVIAIVYKRGE"
     gene            complement(10622..>11749)
                     /locus_tag="PKZ74_05405"
     CDS             complement(10622..>11749)
                     /locus_tag="PKZ74_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPM92228.1"
                     /translation="KIAEKKTVALRKIYADRADLVENEEIINQYIFDVMNIVRPTTRL
                     ESAYDSTLVFEAVSENRALKVKILNQIDQNNPEKPWFFTNTSSVPIHLIDEEAGLGGR
                     ILGFHFYNPPAVQKLVELIVTGQTDAEMTEFAKVYAKNLGKIVVPANDFAGFIGNGHF
                     MRDALHGIQTAQKLTKELTFVEAVYAVNRITQDFLIRPMGIFQLIDYVGIDVVSFIMG
                     VMNPFLPDEDLHSSLLDKMLELGVKGGQYSDGSQKDGFLKYEKGKPVAIYDPQAKAYV
                     PVSSMKEKIDKYLGEAPSSLIPWKAAVKVKDKDATFGKIFADMKMMNSNGAKLAVEYG
                     QRSDAIGKKLVADNVAFNENDVNTVLLTGFFHAYGPVNGYF"
BASE COUNT         2911 a         3062 c         2930 g         2846 t
ORIGIN      
        1 ctccattttc taacaggtaa aggtaaagcc tggcttaata aggcgagaat actgtttgac
       61 ctgggcaatt tccccgaggc gttgaaaaac aacgaggcag cactggaaac ttacctggga
      121 accgatgata aactgggcct tgccaatgct tataacaaca tcggtttgac gaatatttac
      181 ctgggcaatt acgaggaggc catgaaaaat tgcgattcgg ccctggaatt ttatacagag
      241 tccggctcta aaaaaggtca ggccaatgct tacgataata tgggcaacat ttaccagtac
      301 cagcacctgt gggagaaggc agcagaaatg tattttatgt cactgaaaat caatgaagag
      361 atcggtgaca agaatggaat ggccggtaga tatatcaatt taggaaacgt ttatgatgca
      421 atgggcaatc ttccggaagc attaaaccac aacattgcgg cattaaacac ctataaagaa
      481 ttaggcaacg aatataatct ggccatcgtt tataacaata tcgggacttt atattcaaaa
      541 cagaaaaagt atccggaatc cctgcaaatg tttctggaat ccctgcaaat cagcaaggcg
      601 attgaagata cggtgggcat gatcgatcag tacatcaata tcggctggtt gcatttgctg
      661 gagaaaaaat acgctgaatc ggaaaaatat tatcaaaccg cgttagaatt atccgaaaca
      721 ataggttaca gcaaagaaag cacatcccgt tcccattcca atttaggaaa actgtatatg
      781 gaccagggcc ggtatgaaaa tgcggagacc catttcctgg ctgagctgga aatcagcaaa
      841 gagacggaag acctgcaagg cacggccatt gcatataatc acctgggcaa actatatgta
      901 ttatccggtg actttcaaaa ggccgcgagg tttctgaacg agagcctggc catttcacgc
      961 aagcttgatt tgaggaaagg gatcctgaat aatttaaaag atctgtatca gcttgacagt
     1021 gcgcaaggcc atttcgcaaa ggcgcttgaa aattttaaat tatatactgt ttacagcgac
     1081 agtctgctcg gcgaagagac caatcagcag atcaacagac tgaagatcga atacgaaacc
     1141 gaaaagaaag acaaggagat cgaactgctc aacaaaggca atgagatcaa agcgcttcag
     1201 ctcagcaaac aaaaggccat tcggcatggt ctcagcgccg ggttcgtttt gctgttcatc
     1261 accgggttcc tgttgtttcg atccttccgt cttcgaaaga agttggagca gcagcaggcc
     1321 atcatcaacg aacggaaacg gatcagcgcc gatctgcacg acgacgtggg ttcaggcctg
     1381 tcgcgcatca tgctgctgac cgagctggta aagaacgagg ctaaaacgcc agagatgcac
     1441 aaggaagccg aaaagatcgc ctccatctcc aaagaacttt ctgccaatat cagcgagatc
     1501 atttgggcgc tgaactccaa caacgactat ctggaaagcc ttgtggctta cattcgccgc
     1561 tacgcggccg aatttttcga cgattcgtcc gtgagcctga agatcaactc caccggcacc
     1621 ctcaccggaa tacccatcag cggggagttg cgcagggaga tcttctacac cgtaaaagaa
     1681 gcgctacaca atatttacaa gcatgcccag gcctcggagg cgaaactggg attctctgtt
     1741 aaagatgaca ttttaacggt aaccatccat gacaacggca tcggaatgcc ggaaactgtc
     1801 accggccgtt acggcaacgg gctaaccaac atgcagcagc gcatgacggc tgccggaggg
     1861 cagatccgga tcgaaaatca ccagggtacg aagatcatcc tggaggtacc ggtaaagtaa
     1921 tattacccga aagggttatt gaaaaggagg cgtccggcat cttatctttg catcatcatg
     1981 aataaggaaa atatcaccgt tgctattgtt gaagacaccg acgatatccg tgaggcgctc
     2041 cgcgtgctga tcaacggttc cgccggcttc gaatgcgtgc atgtctttgc cagcgcggaa
     2101 gaggcgctcg cgaaaatgcc cgtttccggc atcgacgtgg tgctgatgga catcggtctg
     2161 ccaggcatca gcgggatcga atgcatgaaa acactgcagc cgcgcatgcc ggaaacgcag
     2221 tttatgatca gcacggtgta tgatgatgac gattacatct tcagcgcgct gcaatcgggc
     2281 gccacgggct atatgctcaa gcgcacctct cccgcccaga tcctcgaggc catccgcgac
     2341 ctgaactccg gcggatcgcc catgagcagc gagatcgccc gaagagtggt ggcctacatg
     2401 cagaaaagca aaaggccttc ctccgccgcc gactgcctaa ccgaacgcga aatggagatc
     2461 ctcgatttcc tggccaaagg ctacctttac aaggaaatcg ccgccgagct ctttattagc
     2521 aaggaaaccg tcaaaaagca tatccacaac atttacgaca agctccaggt gcaaacccgc
     2581 accgaggcga tcaataaagc gttcaggaga taacctcacc ccgtatcccc gcgcaagcgg
     2641 agacctgcta tcaatttagc gaatatcaaa aattagtctt ccttcatata accacaaagg
     2701 aacacaatgg ttacactata gttctcaaat actttgtgcg cctttgtggt taaaaaaaga
     2761 attgagggtt acccttccgg caggtgaaaa accgggtgag cttcaaaatc cccctaacgg
     2821 gttatttact tccgcttctg tatttcccaa ttttacattc tgaaacatgc atgtatttcg
     2881 atgcatgatt atatgctttg gatgtgaaat aatctactaa ccaataaaaa ctaaaatcat
     2941 gaaaaagatc attaccagtt taatcatcgc gatctgtacc ttatgggcag ggatcagctc
     3001 atcctttggc caggccttgt tgtatggcgt gacaattgaa ggtggtgcca ataaccaggg
     3061 ggttctttat gagtggaaca cagccatcga tacctacact aaaaagtttg actttggcgg
     3121 agccaatgga acctggccct cctcctcatt gcacttatat gaggggaaat tcatcggaat
     3181 gacttatgaa ggtggagttt acgataatgg agttatttat gagtatgatc ctgtaaatga
     3241 gatgtacaca aaaaaatttg atttcgatgg cacgaatggg tcacagccac tatatgacct
     3301 ggtgttccat gaaggtaaat tttacggaac gaccacttgg ggaggagctt ttaacggtgg
     3361 tgtaattttt gaatgggatc ccttaatgaa cacgtataca aaaaaacatg attttatgga
     3421 tttaggcggc tggacctcta cgcccaacgg caagctggtc atgcacagcg gaaaatttta
     3481 tgggacctgc cagtatggtg ggactaatgg tgctaatggt ttagggtata tttttgaatg
     3541 ggatcctgta accaatgtat ttaccaatgt gttcgatttt gaagaacccg gaggagttgt
     3601 tccgaacagt ctgaccttat atcagagtaa attatacgga actacccaag gtggagggaa
     3661 taattccgtc ggtgttattt tttcatggga tccagccaat gacatttata caacagcaca
     3721 caactttgat tggaacctgg agggtggcac gccgattcat gatttagtgc tcatccagga
     3781 taaattctat ggagtggccg ccaatgccgg tcctcttgat ggcggtgtac tctatgaatt
     3841 tgaaccggtt tcaaccattt acacggtaaa acacgctttt gatctgaata ataccagtaa
     3901 tggttattac cctattggcg gtgtaacctc gagcgggggg aaattgtacg ggactactta
     3961 ctacggaggg ccgtcgggat ttgacttggg tgtattttac gagctggacc cgttgaccaa
     4021 tgtttattcc agtaaatttg aatttacagg gcccgcgaat ggggcttatt ccggttattc
     4081 ttctttactg gaatattttc ccccaacttc tgataatgat ggcgatggtt acacttttac
     4141 cgatggcgac tgtgacgata acgacccgga aatcaatcct ggtgtctccg agctctgcaa
     4201 cggcatcgac gacgattgcg acggggatat tgatgaggga tttgtgtact cggttgccgg
     4261 gacatcgatt acctcctcgg aaggagagaa tttctgcctg ggaaccacca cttatctcag
     4321 cgtgactggc ggcatcctgg gcacgggcgc cgactgggta tggtatgaag ggggctgcgg
     4381 caacagcgat ccgctcggaa cgggtgagac cctacaggtt accccttctg aaggaaccca
     4441 cacttatttc gtgcgtgctg aagggatttg caacacttcc gactgcttca gcctcagcgt
     4501 gactgtcgat aaagcgccgg tgttccagct ttgtcccact gacatcaaca ccggcaacga
     4561 tgcgggatcc tgcttttcaa cagtatacta taacgtcacg gccacaggca gtcctgcgcc
     4621 tgcgttcagt tatgccttcg ccggcgccac caccggaaac ggcacaggca ccggcagcgg
     4681 cagtatcttc aatgccggca ccacaacagt gaccgtaacc gcggagaatt actgcgggac
     4741 ggaaacctgc acattcgacg tgaccgtcaa cgatactgaa gggcctgtga tcagcgattg
     4801 cccgtccggc atcacccttg aggccgaaga aggccagtgg ggcgcttcgg tgagctgggc
     4861 gccgcccacc gcaacggata actgttccgt cactaccctg gcctccagcc atgaacccgg
     4921 acattttttc aatgtcggct ctacacccgt cacttatacc gccaccgatg aagcgggcaa
     4981 cagttctgcc tgccagttcc tggtcacggt gacccctccg gatttcctgg gttcagtcaa
     5041 cctctcgacc atttcctccg aaatcacctt cagcgacaac aatccggccc cgggttcgca
     5101 acttagcgtc aatgccctca tccggaacaa ttccaacatc gatgcaggca gcttttccgt
     5161 tcaattcgcc gacctgtaca ccaatacaac ctacccgttg attccgttgc ccgggttgaa
     5221 tgccgggcaa tcgctcctgg tcagccagtt gattttggcg ccgaatgtgc cgtcgtttgt
     5281 tccgatacag gttgccgtgg atgtgaccaa cagctttgat gaatccagcg aattcgacaa
     5341 tgccgcggtg cggccgttcg tttgcggcga tgtcgcagtg cccggaaata tggacctgct
     5401 ggccagcgca gtgccggcag cggtcccggc gggatcaacc atccagatct gcggcagcgc
     5461 cagctatgat accgatccgc cacttgctga tccatctgtg gccggagcgg aggtgatcat
     5521 ctccatcacg gagaccgggc aacagttcac gggatatacc gatgaagatg gaaatttttg
     5581 catcgcttat gccacaccga atacccccgg cgaatttcat ttcaccgtgg aaatcaccga
     5641 caacatcctt tccggtgatg ccgaaggttc ctttacgctc tcggaaccgg taaatccctg
     5701 tccgacggat ctaagtcttt ccattgacct gcccgggatc ggttcctcct acacgggaag
     5761 cgccatcatt gcagcgggca cctcattctc cggaaccgta ctggtccaga ataactgcac
     5821 cgcgatcaca gaacagacca cgctgttcat cgccccgcca gccggtacgg taaatgcata
     5881 tgaagtgctg gtcccttcgc tgggcccggg ggagacttac cccgtcgccc ttcccgtgct
     5941 gaccatgaac agcactggag atttcttcct cagcgctacc gttgatttca aaaacattgt
     6001 tgaagaagat caggaaaaca ataatactga atacctaaca ctgatcgtta agccggccat
     6061 gccggatatc gtggttgcca aagcggagct ggcttcaaac ctagcctgtt tcgaaaacaa
     6121 cacggtattc tttagcatac tgaacagcgg ccttgtggcc accggcccgt tcacctcggt
     6181 cttaaatttc tatgtcaacg gaatactcca gtcgagccag tcgcaaatca tccaggacct
     6241 cggtccgggc ggtcaaatga cgaggtcgtt cgtgttcacg ctcaccgcac cgggtgacct
     6301 gatcggattt gaactcttca atgatcccgc caatgaagtc gcagagcttt cagaactgaa
     6361 caacgatttt gattttgaaa ccggcttcag gctctgtctg cccgacctgg aagtcagtga
     6421 ctgctattac accgatgtga aaccggccga tgcgatgcag tcaggaatgg ccagcataac
     6481 agccaccatc gtcaaccagg gcgagtcgac ccttaccgta ccgtttgagg tcgccttcct
     6541 ggtcggcagc accaccatca ccgaaaccgt tgacggtccc ctcgcaccgc agcaggccgt
     6601 gcaggtcacc attgaatcgc ccctcccggt tgacgaatgc gtttcgctgt ccatcaccgc
     6661 ggatcctgat gatattgtcc tggaaaaaca gaataacaac aacttcttca gcggttcact
     6721 ctgctatgac ttcgaactga cgaatttcca gtgcgggggt tcgatggttt taccccaaac
     6781 ccagtatttc tgcgatcccg gccagttccg gatcgggtac ctgaaccacg gtttgtacga
     6841 agcgtcagaa ttggaggtcc ggttcgagat cagcggtccg gggcttaccg gatgggtgct
     6901 gctgggaacg gaatccgttt atgccggcaa ctcctgcgga tgtccggtag ttctggattt
     6961 tccgggcagt ttctacttcc cccagaccgg gacctaccag atccggatga cggccgatcc
     7021 agaagatctg tacccggaat ccaatgaagg caacaatgaa ctgaccgtga gctttgatgt
     7081 agttgagaca tctgattacg aggttcttgc tccgtatatc ttcccatcga agatcaaccc
     7141 tgagatcaat gaggcggtct ctttcagtgt ttcctacacc aacaacggct gcgacggcgt
     7201 tacaccgatc gaactgtata cccttgtaga tgaagacccg ctggcttccg tgaccgttgt
     7261 tcctttgctt tccggtgact ggaataccgc agctattccc cagacctggt catcatcggt
     7321 acccggggta catgtgttcc gcgccatcat cgattacagc gaggccattg ccgagggcaa
     7381 tgaaatgaac aacgaagcca ccaaagccat ctatgttggc ggagcgcccg attaccacgt
     7441 cctgtttgtc tcggcatccg atgaaaatcc ggaagccggg cagttggtca cattgacggc
     7501 cgatgttctg aacgaaggcc agtcggcggg gagcgggctc ctgcggttcc tccgtgccga
     7561 tgcccatggt gtggagaccc tgatcagcga agaatccatc agcctggcgc ccggcatcac
     7621 acagagttac agcacctcct ggccggtcac ctatcccggc gctaagatca tcgcccgggt
     7681 ggtgaatgtc ctgcctgctg aattcgacct ttccgacaac gaaaaggaaa cgcagctgaa
     7741 cgatcttcag gtttcgattg ccggaaccaa tgcgctctgc aacggcgaat acagcgggga
     7801 gatcgtcgtg accattaccg gcggcgccgc tcctttcctg atcgaatgga gcgacccggc
     7861 gttgttcggg caggtcgtga atgtacctgc cggggaatac ctggtcaccg ttcttgataa
     7921 cattggcctg agcgctacag ccagtatcac catctctgag ccggaagcac tggtgctgac
     7981 tgaaacccat atcgatgcca gttgtgataa cctgagtgat ggatccgtta atctgggtgt
     8041 cagcggtggc acttcgccat atactatcat atggaataac ggcgagttga cagaagacct
     8101 caccgggctg gaagcaggga catacagtgt actggtaact gatgcaaata attgttctgc
     8161 aacgttatct atcacacttg cagcattgta tgaggctatc gcatggtacc aggatacaga
     8221 caatgacggc tttggaaatg caaatgacag cgtgatttca tgtacccagc cagatggata
     8281 tgtgagcaat cccgacgatt gtgatgacaa taacccggcc atcaatccgg cagaaacaga
     8341 aatttgcggc aacaacttcg acgataactg caacggtcag gttgatgaag gctgtgatac
     8401 agaatgtgat atctccgtca atgccggggc cgatgtcacg acatacttcg ggatcgtatc
     8461 catgcagcaa gtcgtccgca ccgcgcaggt gaccggggga acgcctccgt tcacatacag
     8521 ctggacgatg gaccgcccgc tgctgtgcaa ccagcagaac agcaccggag atgaatcctt
     8581 taccggtgga atatgtactg ataacatctg tccttccagc ggtttaccgg gtgaaaatcc
     8641 aaattgctcg ggcagcgcga ccatctatgc aacgatgctg gatacggctg tggtctgcgt
     8701 aacggtaacc gacgccaatg gatgttcggc cagcgattgc ttcaccgtga tggcttccga
     8761 tgtgaggtgc tttgccggaa acagtggcaa ccacaagata aagatgtgtc acaagacaaa
     8821 cagccagaac aatccgtggg tggagatctg tgttgatacc aattctatca atgcccacct
     8881 ggcccatgga gattatatcg ggtcatgtga tggcgcaaaa tcagataaaa tagaatcaga
     8941 agcggaagat gatttcagct tcgacttcaa tctattcccc aatccagccg aggatcgaat
     9001 cacattatca ttccattgtc ataatgagtc atcctactgg attgaaatca tggatatgac
     9061 cggacgggtt gtcgcgaagt acaaggaaaa ttcctttcat ggcgacatct cgcaccaaat
     9121 agacctaacc ggtctggagc gaggaattta cactgtaact ttaatccttg ggggacaaag
     9181 aatggttaaa aagctgattc gaaattaaga taagcaacgg ttaatttaaa aagggattct
     9241 gacctatagg tcagaatccc tttgcagtta tactactctc cccgcttata cacgattgcg
     9301 atcaccgtcc catcatctcc tcccatattg atcgacatcc catacggttt ttccggatga
     9361 attttgatct gtgctgcgcc ggcgcgtccg gtgagctgtt cccagatggt cacggccata
     9421 tggacgccgg tacctccggt cgggtggccc cagccgatca ggcctccggt cgtgttcatc
     9481 gggatcctgc cgtcgcgggc cgtattgcca tcggctacga actgcgcgcc tttgcctttg
     9541 tcggcaaagc ccagcgcttc ggtagccagc acgccggcta tggagaaaca gtcgtgcgtt
     9601 tcgatagtgc ccaggtcgtt gatcgtgatc ccggcatctt ccagagcggc taatgccgcc
     9661 tttttgatgc aggtcatctc ggtcggatcg ggcggatcct cggtgatatt gcggaccttg
     9721 tgcgcccagg cgatgatctc caccgcgtct ttcttatcga tcccgcaacg tttcagtccc
     9781 tcttcggtac acacaacgat ggctgatgcg ccatcggaaa ctttagaaca atcaagcaca
     9841 ttgaggttgt cgacaaaact ggccccgttg ggcttcatct tgttgtacaa agcttcaagg
     9901 tcttcgactt tgttttcaaa ctcctgcgct gtagggcaaa gccgggcgtt ttcaatggca
     9961 ttgcggaacc atcttcccat tcctttgcgg gcatattcca tcccgtattt ttcataatat
    10021 gcgcctgccc tgtcgctgaa ctggcccgga aagaaatagg catggccgtt tttacgcttt
    10081 tgataccacc ctgcaccggc gaggatatcg gctccataga tggccttgac ggtattctgc
    10141 acttcaattc caaggctcat cgacacttct gcggttccgg ccagaacatt ctttatgctt
    10201 gttaccaggg ccaggctgcc tgaagcacag gctccctcaa cccggatcgc cggtttgtat
    10261 tgtaattccg gatcggccat agggaagaaa ccgccgagat gggcctgctt gttgaagcgg
    10321 gaggccatga agttgcccac cgcgcactcg tcgatgttct ttgccccgcc aacctgcgtc
    10381 aaggttccct gtgccgcttc cttgaaataa tactcgagtc cgggacgctc ctttttcgga
    10441 ttgaactcgc tgcggccggt tcccatcgaa atggtgttgt acccggcagc catgtaaatc
    10501 tttttatgtg gcttgctcat atttcaattg ttctattgtt ctattgttct attgttctat
    10561 tgttcaattg ttctattgtt ctattgttct attgttctat tgttctattg ctgactgccg
    10621 actaaaaata cccgttcacc gggccgtagg catggaaaaa tccggtcaga agtacggtat
    10681 tgacgtcatt ctcattaaac gccacattgt cggctaccag ttttttcccg atagcatccg
    10741 accgctgccc gtattccact gccagctttg cgccattgga attcatcatc ttcatatcgg
    10801 caaatatttt accgaacgta gcatccttgt ctttcacctt caccgctgct ttccagggta
    10861 tcagcgaaga aggggcttca ccgaggtact tatcgatctt ttctttcatc gaactgaccg
    10921 gcacataggc cttcgcttgc ggatcgtaga tggccaccgg ttttcctttt tcatatttca
    10981 ggaaaccgtc tttctggctg ccgtcggagt attgtccgcc tttcacgcca agttccagca
    11041 tcttatccag caggctgctg tgaaggtctt catccggcag gaaggggttc attacgccca
    11101 tgatgaatga aacgacatca atccccacgt aatcgatgag ctggaaaatg cccatcggtc
    11161 ggatcaggaa gtcctgggtg atccggttaa cggcatagac cgcttcgaca aaggtcagct
    11221 ccttggtgag tttttgcgct gtctggatac catgaagcgc atcgcgcata aagtgcccgt
    11281 tgccgatgaa tcctgcaaag tcgttggccg gcacaacgat cttgccgagg ttctttgcgt
    11341 acactttggc aaattcggtc atttcggcat cggtctggcc ggttacgatc agttcgacca
    11401 gcttctgcac agccggcggg ttgtagaaat ggaacccgag gattcgtccg cccagcccgg
    11461 cttcttcgtc gatcagatgg atcggtaccg aggaggtatt ggtaaagaac cagggtttct
    11521 ccgggttatt ctgatcgatc tgattaagga ttttgacctt cagcgcccgg ttttcgctga
    11581 ccgcttcgaa caccagcgta gaatcgtatg ccgattccag ccgtgtcgtc ggccttacga
    11641 tattcatgac atcgaagatg tactggttga tgatctcttc attctcaacc aggtcggcgc
    11701 gatcagcata tattttacgc agcgccactg ttttcttttc ggcgatctt
//
LOCUS       DAOPNH010000073        11748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5765_length_11748_cov_6.415377, whole genome shotgun sequence.
ACCESSION   DAOPNH010000073 DAOPNH010000000
VERSION     DAOPNH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_11748_cov_6.415377"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKZ74_05410"
     CDS             complement(<1..413)
                     /locus_tag="PKZ74_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM92229.1"
                     /translation="MGSAVQSQVFNGGVMAGVAGTQVSGDDYSGFHKAGLFLGGFVNL
                     QFSEHSALQMELEFFQKGSRENPDSTNNYQQYLFRLNYVELPVLYQYTAGKRFKFEAG
                     PSLGFLINYYEENNYYEIKGGNPGARVTFQINAGM"
     gene            634..1320
                     /locus_tag="PKZ74_05415"
     CDS             634..1320
                     /locus_tag="PKZ74_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92230.1"
                     /translation="MEQYRILLVDDEPDILEFLGYNLRREGFEVFTSSNGRDGLAKVK
                     EHKPHLVVLDVMMPEMDGIETCREIRQIPELRNTLVIFLTARGEDYSQIAGFDAGADD
                     YVTKPIKPRVLVSRVKALLRRYKEEAADSGPDQFISTDEFVIDREKYVVIKEGKEFHL
                     PRKEFELLSLLVSRPNKVFTREEILSGIWGDDVIVGDRTIDVHVRKIRERLGTENIRT
                     IKGVGYKYEN"
     gene            complement(1350..1748)
                     /locus_tag="PKZ74_05420"
     CDS             complement(1350..1748)
                     /locus_tag="PKZ74_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92231.1"
                     /translation="MINYDVKILMAFGETIRGNEKIYHWLLKNGYPELAALSSAIRGS
                     QEAVQWLLKNHYPHLAALDAAIDESPKAYEWLSKNNYQLLMVFADACRDKPEAVAWLR
                     KNELDIFVHLAIIIKKWRGEQNFDYHKIHF"
     gene            complement(1750..2526)
                     /locus_tag="PKZ74_05425"
     CDS             complement(1750..2526)
                     /locus_tag="PKZ74_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM92232.1"
                     /translation="MSVLFQDVVFGPVSSRRLGVSLGVNLLPVEAKFCTFNCVYCECG
                     WTHEKSMHGTRLPGMEEVISKLEERLKSMKSAGTRPDAITFAGNGEPTIHPQFPEILE
                     EVIRLRDEYFPGSVVSVLSNSSTLDKPEVFQALLKTGNSIMKLDAGTENMFRLINGPR
                     SGITLKSIVEKLKAFDGELIIQTLFLRGTIDGKQIDNTAEAEISRWMEHIRAIRPKYV
                     MIYPIDRATPLETLEKVSFDELRDIAARVEKMGIKAQVYY"
     gene            2638..3612
                     /locus_tag="PKZ74_05430"
     CDS             2638..3612
                     /locus_tag="PKZ74_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPM92233.1"
                     /translation="MYSLDEIREPIKLNLEEFEKKFRGSMKSSVPLLDTVTRYIYRRK
                     GKQMRPMFVFLTAKMCGEMNESTYRAASLIELLHTASLVHDDVVDDSNERRGFFSINA
                     LWKNKIAVLVGDFMLSRGLLLALEGNDFELLKIVSHAVREMSEGELLQIEKARRLDIS
                     EEVYFEIIRKKTASLIASCCACGASSVGCGPEMTDKMRLFGELVGISFQIKDDLFDFE
                     SGNRTGKPNGIDIKEQKMTLPLIHMLSKLPSSEKRKTIRTIKNHHDDPVRVAAIIDQV
                     NHSGGISYARGKMLEYRQKALDMLDEMPSGEARDALAKLVIYTTERNK"
     gene            complement(3588..4619)
                     /gene="mtnA"
                     /locus_tag="PKZ74_05435"
     CDS             complement(3588..4619)
                     /gene="mtnA"
                     /locus_tag="PKZ74_05435"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPM92234.1"
                     /translation="MLVNGIDYRTIRMEGPSVYMIDQNLLPFEFRIIRTERYTDTCEA
                     IRNMTVRGAGAIGAAAGFAMAQACLEAPASSYAASIEKARIVIESTRPTARNLFYAVE
                     RVFKAGMISVDQAIEEAQKIADEDSGASRRIGEFGSELIREGFRIATHCNAGWLAFVD
                     YGTALSPVYHSFKAGKKIFVYADETRPRGQGARLTAWELHNAGIPHAIIPDNATAWYM
                     AQGKIDLVIVGADRIAANGDTANKIGTLEKAIAAKHYGIPFFVAAPTSTFDPLCPDGS
                     HIAIEERNPDEVLYQEGLTKDGKRERVLVCSPGSPALNPAFDVTPAGLITGYITEQGV
                     VDYLFRSVV"
     gene            complement(4643..5203)
                     /locus_tag="PKZ74_05440"
     CDS             complement(4643..5203)
                     /locus_tag="PKZ74_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92235.1"
                     /translation="MRKVFLMSVLIMMAGLAGMAQSFEKGSQAVNIGIGLGPGWRSSS
                     LGFSFGVNGSYEYGIVEVPMGSTLTGVVGVGGIAGAGFSGATYGLVDNYSSVNYFVAA
                     RGNYHFIFHDDFDPYASILIGFHGYSDKWKGDGPEPPKTTYSGGDFRAGVVVGARYLF
                     SDTFGVYAELGYLLHVFNAGVTIKLK"
     gene            complement(5257..6459)
                     /locus_tag="PKZ74_05445"
     CDS             complement(5257..6459)
                     /locus_tag="PKZ74_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPM92236.1"
                     /translation="MPTLKNRIKRLAKGYLPEITQMRRHFHMFPELSGKEIQTAAFIS
                     KQLSEWGIAHKTGIAGNGILAVITGELPGKKVVALRADMDALPIKELNKVAYASRTKG
                     VMHACGHDAHMASMMGTARLLKSLEAEFGGTVKIIFQPSEEKYPGGALPMIKAGVLEK
                     PMTDLIIGQHVFPELEAGKVGFKSGQYMASTDEIHLTVKGKGGHAAIPDKVIDPVLIA
                     AHIIVALQQIVSRNAKPTMPTVLSFGKVMADGVANIIPDEVKISGTMRTFDEGWRVEM
                     KERITDMATALAKSMGGTCEVDIHKGYPFVYNDPALTTRAKKLSEEYLGRDKVVDLEM
                     RMTAEDFAYFAQEVPGCFYRLGVMNESKGITSNLHSATFDVDESSLETGVGLMTWIVM
                     NELGQNRE"
     gene            complement(6525..7208)
                     /locus_tag="PKZ74_05450"
     CDS             complement(6525..7208)
                     /locus_tag="PKZ74_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM92237.1"
                     /translation="MDQKKTTKADLENKKIIFRQIGLVIALAAVLLAFEWKTYEKSVV
                     DLGTRQVEDIAEDIIPITEQKIKPPPPAPPKQVIQINVVEDDIEVEDDIDIDVEADEN
                     TEVQEYVAPVKVEEEESAEEVQIFMVVESMPEFPGGEPALYKFLAENIKYPQMAKESG
                     IQGRVFVTFVVERDGRVTDVRVLRGIGGGCDEEAIRVVQNMPKWTPGKQRGKAVRVQY
                     NLPVKFTLQ"
     gene            complement(7320..7700)
                     /gene="gcvH"
                     /locus_tag="PKZ74_05455"
     CDS             complement(7320..7700)
                     /gene="gcvH"
                     /locus_tag="PKZ74_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPM92238.1"
                     /translation="MNIPGNLKYTKDHEWMKPEGDIAVVGITDFAQNELGDIVFIEVE
                     TIGESLDKEETFGTIEAVKTVSDMFMPVAGEILEFNQALVDKPDLVNKDPYGEGWIVK
                     IKMADAGQAAELLDAGQYGELVGA"
     gene            complement(7800..>11748)
                     /gene="sprA"
                     /locus_tag="PKZ74_05460"
     CDS             complement(7800..>11748)
                     /gene="sprA"
                     /locus_tag="PKZ74_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPM92239.1"
                     /translation="VHGTASIAYFSALEDPSADDYNYFRDSDYDNDDKYSTILERYKK
                     YNGPDGNSPTDSQNDEEYPTSATAIPNVEDLNRDNTLSEAERYFQYVIDLDPNKMEVG
                     ENFITDIRTAKGVPLANGTTGEVNWYQFKVPVAQPSRVVGNISDFKSIRFLRMFMKDF
                     EKPVTLRFATLELVRGEWRRYKGVLLSAGEYIPDDIQSLTTFDIFSVNIEENGRRQPV
                     PYVLPPGLEREQNIGTTTLVRLNEQSMVLKVCDLIDGDSRAAYKTTDFDFRQYKNLEM
                     FVHAEKSRNEDVVNDGDLTVFVRIGSDFTENYYEYEVPMTFTPWGTPPSQADVIWPES
                     NRFNIDLEKLVQVKLDRDIAARNGESNVSVNFPYVRQEGPNRITVLGVPSVSDVMAIM
                     VGVRNPKRQSLTGEDDGLSKCAEIWINELRLTGFNDKAGWAGLIRVNSQLGDIGQVVF
                     STMYSSPNFASLEKKINDTQKESHLQWDIATDLNLGKFLPEEAGVRIPMHIDYSQTIE
                     TPEYDPLSPDLKLKDELASYENKEEADSIRDIVQDYTDRFNINFMNVRKDRVGAKKKP
                     QFYDVENFSVSYAYTMLNMRNIDIEYDKKETHNGGLAYNFNIAPKNIKPFQNIGFISK
                     SEFLKLFKDFNFYFLPKQFGFRTDMMREEAERKLRNKSEGLILLRPTFARKWDWNRLY
                     DLKFDLATSLTLQYRASVNSFLREPSGSLDPNSPWYDKAGADTIDVGRQFWQGGLRRN
                     YNQTLDITYRIPIDKLPMMDWVTAQATYGTIYNWIASPLSVQQRLGNVIENSRNIQLS
                     GNVDLTKLYGKVPYLKKLGQSAKPDTRGRSGTVPKQQPPPQEEQDSVAKPNYFKKVAD
                     EFLKFVMMVKRGSITYSESTGTLIPGFMPSPQALGVDWGLNAPGLGFAFGSQKDIRSQ
                     AVQERWLTYDTLQNQAYANKFTSSLNARVSLEPFQGFKIEINADRTFARNHTEYFRAD
                     SSGVFDSYSPQDAGSFSMSYITWGTAFHTDYDTSISATFEEMKRNRYDIAMRLAAENP
                     NSNAMVYDSLSQQYYPLGYGPTSQDVLIPAFLAAYGNKSTTEVYLGYFPSIPLPNWRV
                     TYDGLTKIKFLQKFIKSAVLSHSYRSVYTISSYQSNLYYGETDGKPSALYEASNSYYP
                     KYDLNQVTIQEQFAPVVGIDITLNNSLLTRFEFRKARTLTFSMINKQLTDLSTDEYVI
                     GLGYRIKDVSFTVSSLGGGGRKTQLKSDLDIKVDFSIRNNRTVLRRIDENIDQVSAGQ
                     RVYSINTSIDYMLSKSVTLRLFYDMIINRPFVANQYDNSTTRAGISIRFSLAQ"
BASE COUNT         2804 a         2971 c         2865 g         3108 t
ORIGIN      
        1 tacattccgg cattgatctg gaatgtgacc ctggcgccgg ggtttccccc ttttatctca
       61 taataattat tctcttcata ataattgatc aggaacccga gcgaaggccc cgcttcgaat
      121 ttaaaccgct tacctgccgt atactggtat aaaacaggca attccacata attgagccgg
      181 aataaatatt gctggtaatt attggtagag tctggatttt cgcggctccc tttctggaaa
      241 aactcaagct ccatctgcag tgcggagtgt tccgaaaact gcaggttgac aaatccgccg
      301 agaaaaaggc ccgctttatg gaacccggaa taatcatctc cggaaacctg tgttcccgcc
      361 actcctgcca taactccacc gttgaatacc tggctttgta cagccgaacc catcagaaaa
      421 aaacttaacg taaaaataat atatctcaat tgcctcatta taactgccaa ttaatcataa
      481 accaaacggc aaatatcgta attttaagct gttagacccg ataaaggcgt ctgctcctgg
      541 ataaaatagt aatttcgctg ccgaatgtct tttattaatg actgttgcag aagggtttaa
      601 ccaattataa tcataaccag atcaatttcc tttatggagc aatacagaat tttattagtg
      661 gatgacgaac cggatatcct ggagtttctc gggtataacc ttcgccggga aggatttgaa
      721 gtatttacca gcagtaacgg cagggacgga ttggcgaagg taaaagaaca caagccccat
      781 ctggtcgtgc tcgatgtcat gatgcccgag atggacggaa ttgagacctg ccgcgagatc
      841 aggcagatcc cggagctgcg gaatacgctg gttatttttc tcacagcccg tggtgaagat
      901 tattcacaga ttgccggttt cgatgccgga gcggatgact atgtgaccaa accgatcaag
      961 ccccgtgttt tggtaagccg cgtcaaggca cttctccggc gttataagga agaggctgct
     1021 gattcaggcc cggaccagtt tatcagtacc gatgaatttg tgatcgaccg tgagaaatat
     1081 gtcgtgatca aagaaggcaa agaatttcat ctccccagga aggaatttga attgttgagc
     1141 ctgctggttt ccaggccgaa caaggtgttc acccgtgaag agatcctttc gggtatctgg
     1201 ggcgatgatg tcatcgtcgg cgaccggacg atcgacgtac atgtcaggaa gatcagggaa
     1261 cggctgggaa cagaaaacat ccggacgatc aaaggcgtcg gctacaaata cgaaaactaa
     1321 tctggaacct ggaacttaag aacctggaac taaaaatgta tcttgtggta atcaaaattc
     1381 tgctcaccac gccatttttt aatgataata gccaggtgga caaagatatc cagctcattt
     1441 tttctcagcc aggccaccgc ttccggttta tcgcggcagg cgtcggcgaa aaccatcaat
     1501 agctgataat tgtttttact cagccattca taggctttcg gcgattcgtc gatagccgca
     1561 tcgagggcgg ccaggtgcgg gtaatggttt ttgagcaacc attgcacagc ctcctgcgaa
     1621 ccccggatgg cgcttgaaag cgctgccagc tccgggtacc cgttcttgag cagccagtgg
     1681 taaatctttt cgttacccct gatcgtctcg ccgaaggcca tcagtatttt tacatcataa
     1741 ttgatcatac taataataaa cctgcgcttt aatacccatt ttttcaaccc gggcggcaat
     1801 atccctgagt tcgtcaaaag agactttctc aagagtctcc agcggcgtag cgcggtcgat
     1861 gggatagatc atcacatatt tcggccgtat ggcccggata tgctccatcc acctcgagat
     1921 ttccgcttct gccgtattat cgatctgctt tccgtcgatg gtgccccgga gaaagagtgt
     1981 ctggatgatc agctcaccgt cgaaagcctt taacttctcc acaatggact tcagcgtaat
     2041 gcccgaccgt gggccgttga tcagccggaa catattttcg gtcccggcat ccagtttcat
     2101 aatgctgttg cccgttttca gcagagcctg gaagacctcc ggtttatcca gcgtggagga
     2161 gttcgaaagc accgagacca cagaaccggg aaagtattcg tccctgaggc ggatcacctc
     2221 ttccaggatt tcggggaact gcgggtgtat ggtcggctcg ccgttgccgg cgaaggtgat
     2281 cgcatcgggc ctggtaccgg ccgatttcat ggatttaagt ctttcttcca gttttgagat
     2341 tacttcttcc attccgggca gccgggtacc gtgcatgctt ttttcgtgag tccagccaca
     2401 ttcgcaatag acgcaattga aagtgcagaa cttggcttcc acgggcaaca ggttaactcc
     2461 cagcgaaaca cccagccggc gactgcttac cggcccgaaa accacatcct gaaatagtac
     2521 cgacataacg actggccttg caataacatt tatttcacaa agttaaacaa atcaggcttc
     2581 cggatggttc tgtaccttac tccttttatt attacttttg atccgttgta aggcattatg
     2641 tacagtcttg atgaaataag ggaacctata aagctgaacc tggaagaatt tgagaagaag
     2701 ttcaggggat cgatgaagag ttccgttcct ttgctggata ccgttacccg atatatatac
     2761 aggcgtaaag gtaagcagat gaggccgatg ttcgtttttc tcacggccaa gatgtgcggg
     2821 gagatgaacg aatcgactta ccgggctgcc tcgctgattg agcttttaca tacggccagc
     2881 ctggtgcacg acgatgtggt ggatgattcc aatgagcggc gcggattttt ttctatcaat
     2941 gccctgtgga aaaacaagat tgccgtcctg gtgggcgact tcatgctgtc gcgcggcttg
     3001 ctgctggccc ttgaaggcaa tgatttcgag ctgctgaaga tcgtttcgca cgccgtcagg
     3061 gagatgagcg aaggcgagct cctgcagatc gagaaagccc ggcggctgga tatttccgag
     3121 gaggtctatt tcgagatcat ccggaaaaag acggcttcgc tgatcgcctc ctgctgtgcc
     3181 tgcggggcat cctcggtggg ctgcggaccg gaaatgacag acaaaatgag gttgtttggc
     3241 gagcttgtgg gtatatcttt ccagatcaaa gatgacttgt tcgatttcga atcgggtaac
     3301 cgcacgggca agccgaacgg gatcgacatc aaagaacaga aaatgacgct gccgctgatc
     3361 cacatgctca gtaaattacc gtcatcggag aagagaaaaa cgatccgcac gatcaagaat
     3421 caccacgatg atccggttcg ggtagcggcg atcatcgacc aggtgaacca cagcggcggg
     3481 atcagctatg cccgtggaaa aatgctcgaa tacaggcaaa aagccctcga tatgctcgat
     3541 gagatgccat cgggagaagc ccgcgatgca ctggccaagc tggtgatcta cacgacggaa
     3601 cggaataaat agtcgaccac tccctgttcg gtgatatagc ccgtgatcag tcctgccggc
     3661 gtgacgtcga aagcagggtt cagcgcgggc gatccggggg agcaaaccag caccctttcc
     3721 cgttttccat cttttgtcaa cccctcctgg taaagcacct catccgggtt gcgctcttcg
     3781 atggcaatat gcgatccgtc ggggcaaagg ggatcgaagg tggatgtcgg ggcggccacg
     3841 aagaacggga tgccataatg tttagccgcg atcgcttttt cgagcgtgcc gatcttattg
     3901 gccgtatcgc cgttggccgc aatccggtca gcccccacga tcaccaggtc gatcttaccc
     3961 tgagccatgt accatgctgt ggcattatcg gggatgatgg catgcggaat gccggcatta
     4021 tggagctccc aggcggtaag cctcgccccc tggccgcggg gcctcgtttc atcggcatag
     4081 acgaagatct ttttgccggc cttgaagctg tgataaacgg gtgagagggc cgtaccgtaa
     4141 tcgacgaagg caagccagcc cgcattgcag tgggtggcaa tacggaaacc ctcccgtatc
     4201 agttcgctgc caaattcccc gatccggcga gaggctccgc tgtcttcatc ggcaatcttc
     4261 tgtgcttctt caattgcctg atcaactgat atcattccgg ctttaaagac tctttcgacg
     4321 gcatagaaga ggttccgggc cgtcgggcgg gtagattcga tgactatccg ggctttttca
     4381 atggaggcgg cataagaaga ggcaggcgct tcgaggcagg cctgtgccat ggcaaaaccg
     4441 gccgcggcgc cgatagctcc ggcgccgcga acggtcatgt tgcgaatagc ttcacacgta
     4501 tcggtatacc tttcggtacg gataattctg aattcaaatg gcagcagatt ctggtcaatc
     4561 atatacacgg acgggccttc catccggatc gtccggtaat ctatgccatt gacaagcatt
     4621 gcatctattg aaatcgaaaa tattacttca gtttgatggt aacacctgca ttgaatacat
     4681 gcagcaggta acccagttcg gcatagacgc caaaggtatc agagaacaaa tagcgggcac
     4741 cgaccacaac gccggccctg aaatcgccac ctgaataggt tgttttcgga ggttccggac
     4801 catcgccctt ccatttatcc gaataaccat gaaatccaat taatatcgat gcatagggat
     4861 caaaatcatc atgaaaaata aagtgataat tacccctggc agccacaaag taattgacag
     4921 aactgtagtt atccaccagt ccgtatgtcg ctccggaaaa tccggcgccg gcaataccac
     4981 cgacacccac gacgccggtc aatgtggaac ccatcggtac ttcgactata ccatattcat
     5041 aagatccgtt gacgccaaag gaaaagccaa gactactact ccgccagccc ggtcccaggc
     5101 cgatcccgat atttaccgcc tgcgagcctt tttcaaagga ttgggccatt ccggccaaac
     5161 cagccatcat aatcaacact gacatcaaaa atacttttct cattttctat cgttttaaaa
     5221 ttagacttta agtgaattaa tagtcaaaaa taatacttat tcccgattct ggcccagttc
     5281 gttcatcacg atccaggtca tgaggccaac gcctgtttcg agggatgatt catccacgtc
     5341 aaacgtggcg gaatgcaggt tggatgtgat ccctttgctt tcgttcatta cgccgagccg
     5401 gtaaaagcaa ccggggactt cctgcgcaaa ataggcgaag tcttcagccg tcatacgcat
     5461 ttccagatcg accaccttat cgcggcccag gtattcctcg gacagtttct ttgcccgcgt
     5521 tgtaagcgcc gggtcattat acacaaacgg gtaacctttg tgaatatcca cttcacaggt
     5581 gcctcccata gattttgcca gggccgttgc catatccgta attctttcct tcatctcaac
     5641 gcgccagcct tcgtcgaaag tgcgcatggt gcccgatatt tttacctcgt cggggataat
     5701 attggcgacc ccgtcggcca tgacctttcc gaatgacagt acagtgggca tggtcggttt
     5761 ggcgttgcgg ctgacgattt gctgcagtgc gacgatgata tgggccgcta taagcaccgg
     5821 atcgatcact ttatcgggaa tggcggcatg gcctcctttg cctttcaccg tcaggtggat
     5881 ttcatcggtg gatgccatat attgcccgct tttaaagccg acttttccgg cttccagttc
     5941 ggggaagaca tgctgtccga tgatcaggtc ggtcatgggc ttttcaagca caccggcttt
     6001 gatcatggga agggcgccgc cgggatactt ttcttccgaa ggctggaaga ttatttttac
     6061 tgtaccgccg aattcggcct ccagtgattt cagcaagcgg gccgtgccca tcatggaagc
     6121 catgtgtgcg tcgtgtccgc aggcatgcat cacccctttg gtccgcgagg cataagccac
     6181 tttgttcaac tcttttatcg gcaaagcatc catatcagcg cgaagggcga ccactttttt
     6241 cccgggcaat tcaccggtaa tgactgccag aatgccgtta ccggcaattc cggtcttatg
     6301 ggctattccc cactccgaaa gttgtttcga aataaaagcg gctgtttgga tctctttgcc
     6361 cgacagttcg ggaaacatat gaaaatggcg ccgcatttgg gttatttcgg ggagataccc
     6421 tttggccagg cgtttgattc tgttttttag tgttggcata agctaatgga aaaaaaggcc
     6481 atcttttcag taagacagcc tttttcattt ataaaggata atctttattg cagtgtaaat
     6541 ttaaccggca ggttgtactg gacacgaacg gcttttccgc gttgtttccc gggggtccat
     6601 ttcggcatat tctggactac ccggatcgct tcttcgtcgc atccgcctcc gatacccctg
     6661 aggactctta catcggtgac ccggccgtcg cgttcaacga cgaaggtgac gaatactcgt
     6721 ccctggatac cgctttcttt cgccatctgg gggtacttta tattttcagc gagaaattta
     6781 tagagggccg gttctccacc cgggaattcc ggcatggatt cgacgaccat aaaaatctga
     6841 acttcttctg ctgattcttc ttcttcgacc ttcaccgggg ccacatattc ctgtacctcg
     6901 gtattttcat cggcttcaac gtcgatatca atatcgtctt ccacttcaat atcatcttct
     6961 acaacattga tttggataac ctgcttgggt ggagccggtg gtgggggttt tattttctgt
     7021 tcggtaatcg ggatgatatc ttccgcaata tcttccactt gtcttgtgcc cagatcaacg
     7081 actgatttct catacgtttt ccattcaaag gccagcagca ctgcagccag tgcaataaca
     7141 agtccgatct gccggaaaat gatctttttg ttttccagat cggctttggt tgtttttttc
     7201 tgatccatgt cttttattct taagattaat tattatggat tcgatgtaaa agtataacaa
     7261 aaaaatattt tgtcaacttt tcactcataa tatattcaaa aactatacca ggaatgtcgt
     7321 caggcaccga caagttcccc gtattgtccg gcatccagca attcagccgc ttgccctgca
     7381 tcagccatct ttatcttaac gatccagcct tcgccataag ggtccttatt gaccagatcc
     7441 ggtttatcga ccagggcctg gttgaattcc aatatttcac cggcaaccgg cataaacata
     7501 tcagatacgg ttttcacggc ttcgatcgta ccgaaggttt cttctttatc caggctttcg
     7561 ccgatggttt caacttcaat gaatactatg tcaccaagct cattctgagc aaaatcggtg
     7621 ataccgacca cggcaatgtc gccttccggt ttcatccact catgatcttt cgtgtacttc
     7681 agatttccgg gaatattcat gttattattc ttaaaaattt cggcttcaaa tttaaagcaa
     7741 tttcaaaata tagccaccat tcggattagt ttaaaatgat tccacatacc gggcagaagt
     7801 tattgtgcca gtgagaaccg gatcgagatg cctgccctgg tggtggaatt gtcgtattgg
     7861 ttggctacaa acggccggtt gatgatcatg tcgtaaaaca atctgagtgt gacgctcttg
     7921 ctcagcatgt aatcaatcga ggtattgatg gaataaaccc gctgtccggc cgagacctgg
     7981 tcgatgtttt catcgatcct tcgcagaacc gtccggttgt tacggatcga gaaatcgact
     8041 ttaatatcca gatcgctttt caactgggtt ttccggcctc ccccgcctaa tgagcttacg
     8101 gtgaatgaaa cgtctttgat ccggtagccc agtcctatca catattcgtc ggtactcaga
     8161 tcggtgagct gcttattgat catgctgaaa gtcagggtgc gggccttccg gaactcgaac
     8221 cgtgttagga ggctgttatt cagcgttata tcgattccta cgactggtgc aaattgttcc
     8281 tggatcgtca cctggttcag gtcatatttt ggatagtagg aattggatgc ctcatacaat
     8341 gccgacggtt tcccgtcagt ctctccataa tacaggtttg actgatagga acttatggta
     8401 taaaccgatc ggtaggaatg tgataaaaca gccgatttta tgaacttttg caggaattta
     8461 atcttggtca ggccatcgta agtgactctc cagttcggga gggggatgct cgggaaataa
     8521 cccaggtaaa cttccgtcgt gcttttattt ccgtaagcgg ctaaaaatgc cgggatgagc
     8581 acgtcctgcg acgtcggccc atagcccagc ggataatact gctgagaaag ggaatcgtag
     8641 accatggcat ttgaattcgg attctcagcg gccagcctca tggcgatatc ataccggttt
     8701 ctcttcattt cttcaaatgt cgctgagatc gaggtatcgt aatcggtgtg gaatgccgtt
     8761 ccccaggtga tgtacgacat gctgaaacta ccggcatcct gaggggaata gctatcaaag
     8821 accccgctgg aatcggccct gaaatactct gtatggttgc gggcaaaggt ccggtcggca
     8881 tttatctcaa tcttaaagcc ctggaatggc tccagtgaaa cacgggcgtt caggctggag
     8941 gtgaatttat tggcatacgc ctggttttgc agggtatcgt aggtgagcca cctttcctgc
     9001 acagcctgcg accggatatc tttctggctt ccgaaggcaa atccaaggcc cggcgcattc
     9061 agcccccagt cgactcccag cgcctgcggc gatggcataa aaccggggat cagcgtccct
     9121 gtgctttccg aataggtaat gcttccccgc ttgaccatca tgacaaattt gagaaattca
     9181 tccgccactt tcttgaaata gttgggtttg gccacactgt cctgttcctc ctggggaggc
     9241 ggctgttgtt tcggcacggt ccccgaccgt ccgcgtgtat cgggtttggc cgactggccc
     9301 agttttttca ggtaggggac ttttccataa agtttggtca ggtcgacgtt cccggataac
     9361 tggatgttcc ttgaattttc gattacattg ccaagcctct gctgcactga aagcggcgac
     9421 gcaatccaat tatagatcgt tccgtaggtg gcctgtgccg taacccagtc catcatcgga
     9481 agcttatcga tcggaatccg gtaggtgata tcgagggtct ggttgtaatt ccggcgcagt
     9541 cctccctgcc agaactgtct gccgacatca attgtatcgg cgccggcttt gtcgtaccag
     9601 ggagaattgg gatccagcga cccggacggc tcccggagaa acgagttaac ggaggcccgg
     9661 tactgcagcg tcaggctggt cgccaggtca aatttcaggt cgtacaaccg gttccagtcc
     9721 cacttcctcg caaaagtcgg acgcaataat atcaatcctt cgctcttatt cctgagtttt
     9781 cgttccgctt cttccctcat catatcggtc cggaatccaa actgcttggg cagaaaatag
     9841 aaattaaagt ctttaaacaa cttgagaaac tccgatttgg agataaaacc tatattctga
     9901 aatggcttga tatttttcgg tgcaatattg aaattatagg caagtccccc gttatgcgtt
     9961 tcctttttat cgtattcgat gtcgatattc ctcatattca gcatggtata agcataactt
    10021 acgctgaaat tctccacatc atagaattgt ggtttctttt tagcccctac cctatccttg
    10081 cggacattca tgaaattgat attgaaccgg tcggtataat cctggacaat atcccgtatc
    10141 gaatccgctt cctctttgtt ttcataggat gccagttcat ctttcagttt caggtcgggg
    10201 ctgagagggt cgtattccgg ggtttcgatc gtctgcgaat agtcgatatg catggggatt
    10261 ctcacgccgg cctcctcggg gaggaatttc ccgaggttca ggtcggtggc gatatcccat
    10321 tgcaggtgcg attccttttg agtatcgttg attttcttct caagggaagc aaaattgggt
    10381 gaactgtaca tggttgaaaa gaccacctgg cctatatcgc cgagctggga attaacgcgg
    10441 atcagtcctg cccacccggc tttatcatta aaccccgtca gcctgagctc attgatccag
    10501 atctcggcac atttcgacag cccgtcgtct tcaccggtca gcgattgccg tttgggattc
    10561 ctcactccga ccatgatcgc catgacatca ctgaccgaag gaacgccgag cactgtgatc
    10621 ctgtttggcc cttcttgcct gacgtaggga aaattgaccg acacattgct ttccccgttt
    10681 ctggctgcaa tatcccggtc gagttttacc tgcacgagtt tctcaaggtc gatattaaac
    10741 ctgttcgatt cgggccagat gacatccgcc tgagagggag gcgttcccca gggtgtaaag
    10801 gtcatcggca cctcatattc gtagtagttc tcggtaaaat cggaaccgat cctgacaaaa
    10861 accgtcagat cgccatcatt gacaacatct tcattcctgg atttctcggc atgcacgaac
    10921 atttccaggt ttttatactg ccggaagtcg aaatcggtgg ttttgtaagc tgcccgggaa
    10981 tcgccgtcga tcaggtcaca aactttcagt accatcgatt gttcattcag ccggaccagg
    11041 gtagtggtgc caatattctg ctcccgttca agcccggggg gcaggacata cgggaccggc
    11101 tggcgacgtc cgttctcttc aatatttaca ctgaaaatat cgaaagtggt caggctctgg
    11161 atatcatcgg ggatatattc gccggccgaa agcaaaacgc ctttatagcg ccgccattcg
    11221 cccctgacca gctccagggt ggcaaacctt aatgtgaccg gtttttcgaa atccttcatg
    11281 aacatcctga ggaagcggat cgatttgaaa tcgctgatat tacccaccac ccggctgggc
    11341 tgggcgaccg gtaccttgaa ctggtaccag ttaacctcac cggtcgtacc gttggccagc
    11401 ggcactcctt ttgccgtgcg gatgtcggtg ataaaattct caccgacctc cattttattc
    11461 gggtcaagat cgatcacata ctggaaatag cgttccgctt cgctgagtgt attgtcgcgg
    11521 ttcaggtctt ccacattggg gatcgccgtg gccgatgtcg ggtattcctc atcgttctgc
    11581 gaatccgtag ggctgttgcc gtcgggcccg ttatactttt tgtacctttc aaggatagtg
    11641 gaatatttat catcattatc ataatccgaa tccctgaaat aattataatc gtcggctgac
    11701 ggatcttcca atgcgctaaa gtaggctatc gatgccgtac catggacc
//
LOCUS       DAOPNH010000074        11734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5774_length_11734_cov_5.532323, whole genome shotgun sequence.
ACCESSION   DAOPNH010000074 DAOPNH010000000
VERSION     DAOPNH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_11734_cov_5.532323"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKZ74_05465"
     CDS             <1..981
                     /locus_tag="PKZ74_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPM92240.1"
                     /translation="HLHLMVYGDLVQSPQVLLAEKLASCLPSALRSVYFVTSGSEAIE
                     GALKLAKRYTGRTGVVAFHKAYHGGTHGALSICGDERLKTAFRPLLPDITHIKFNRFE
                     DLDQITDKTACVVVETIQAEAGIVLPAAGFLEALRAKCTETGALLVIDDIQMGMGRTG
                     KLFSFEHCGIVPDILCLAKAFGGGMPLGAFISSKYIMQSLAHDPELGHITTFGGHPVS
                     CAAALASLNYLVNTGIFEAAEQKAQMFIESLAGHPAIKTIRHKGLMIGIDLESIEMNQ
                     KLMKAFHENGLIADSFLFRPQAFRIAPPLIITEEEIEMTCERIIASLNRL"
     gene            complement(1065..2276)
                     /locus_tag="PKZ74_05470"
     CDS             complement(1065..2276)
                     /locus_tag="PKZ74_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92241.1"
                     /translation="MRTIFRYTGIFFLIFSGAGQTLAQWAANGNNIYNTNSGNVGIGT
                     NSPATLLHVAKSMTEPTITVQNLGGSGGATYVMLDNASGANWKFKATLSGGFKIRDHA
                     NLMDVIVIEPNSFANAIYISGSQSIGIGTSNPNSSALLDISSNVKGFLPPRMTLAQIE
                     GIPGPASGLMVFCTTDEKLYIYVNSAGLWKEVPYGTETINPNETCGSPIVRNHVAGTV
                     APVDKTVTYGTVLSDLSGIQQCWITQNLGADYQATTVNQTTEPPAGWYWQFNRQQGYK
                     HDGSVRTPSTAWITDITENYDWQPENDPCYLLLGTGWRLPTNTEWTNADINGEWDNAN
                     EAFASVLKLHMAGVLTDATGNLAARGTDGDCWSSTQNSTTQSKMLHYSTSNCQIQNSN
                     KEFGLNVRCIK"
     gene            2731..3063
                     /locus_tag="PKZ74_05475"
     CDS             2731..3063
                     /locus_tag="PKZ74_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM92242.1"
                     /translation="MATPAVNIIESKDDYRIEVAAPGLDKADFRINIENNILTISAEK
                     EEKQEEKDEKVMRREFNYSSFSRSFTLPDSMDTEKISARHKDGVLNVILPKKEEAKEK
                     PARTIKIS"
     gene            3194..3843
                     /gene="aat"
                     /locus_tag="PKZ74_05480"
                     /pseudo
     CDS             3194..3843
                     /gene="aat"
                     /locus_tag="PKZ74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
     gene            3857..4336
                     /locus_tag="PKZ74_05485"
     CDS             3857..4336
                     /locus_tag="PKZ74_05485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPM92243.1"
                     /translation="MSDEQNREFMEEAIRLAALNMDATHGGPFGCVIVKDGRIIASAY
                     NEVLRTNDPTAHAEIVAIRRACEKLNSYQLAGCEIFTSCEPCPMCLGAIYWSRPAKVY
                     YAASRKDAEKAGFDDNHIYQEVAVPDQNRVIPFEQFMPDKVVKLFQQWVQLKRDVNY"
     gene            4320..5258
                     /locus_tag="PKZ74_05490"
     CDS             4320..5258
                     /locus_tag="PKZ74_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM92244.1"
                     /translation="MTSTINARQELNRTFKTHIALLGANFIYGLNYVIAKGIMPDHLS
                     PKSIIFIRVIITVVIFWILHLFIPSEKVEKRDLFKFALCAFFGVAVNQLLFFEGLNLS
                     TPINASIIITVIPVLVLLFSHFILHDKITPIKVAGIIMGAAGALMVILSAGAGDFRSA
                     TMLGNLLIFINAASWSFYLVLIKPLMERYDNFTVMKWVFLFGFIIIFPFTFNSFASSS
                     FSTIPVNIWLSISFVVFGATIIAYFLNNYSLKSVSPSVNGIYIYLQPLIASVVSILFG
                     KDQISVIKILAAILIMTGVYLVTRHSRKTASIFPEQ"
     gene            5309..5989
                     /locus_tag="PKZ74_05495"
     CDS             5309..5989
                     /locus_tag="PKZ74_05495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM92245.1"
                     /translation="MKKYYLSAVLLFALTLSASAQDTVKVALKTSADSLSFSFGYLIG
                     NNLFVQNVENINQKIFMKGFNYGYSDQKTTLSVEEANRIIQHYFQNMISDESKQNLEK
                     SNAFLEENKLKEGVTTLPSGLQYTIITAGTGESPKPEDKVKVHYTGHLIDGTVFDSSV
                     ERGEPIVFGVNQVIPGWTEALQLMQPGAQWILYIPPALAYGENGAGGVIGPNQALIFE
                     VELIEVVK"
     gene            complement(5982..6950)
                     /locus_tag="PKZ74_05500"
     CDS             complement(5982..6950)
                     /locus_tag="PKZ74_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM92246.1"
                     /translation="MKEIRDHISRLDFEQLVKPIEELKNCRICPRNCNADRFSDKRGY
                     CKAGTDFTISSIFPHRGEEPVISGDRGICNIFFTNCNLQCIYCQNHQISCNSLDYGGN
                     RLSLPEVLERIATILATGINHVGFVSPGHFIPHVKVIVNALREMGSDPVFVYNTNGYD
                     TVESIRGLEGYIDIYLPDYKYSDENLGRKYSDVKNYPTVALEALKEMVRQKGTGLPVD
                     NRGYATNGVIVRHLVLPGHPVNSINVLRTIAYEVSNQLHISLMSQFYPTYLVQNHESL
                     GRTISEKEYQRVTEALHKLGFSKGWVQDFSSHESYRPDFEEETPFI"
     gene            7185..9827
                     /locus_tag="PKZ74_05505"
     CDS             7185..9827
                     /locus_tag="PKZ74_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM92247.1"
                     /translation="MTSKFPVTYPLLTFRLDNELISLADAKAYCTDSSIIFDYKGLIT
                     GEIIADNEFQEVWQRRVILRNPTDDTIDIENLVPFGEIPDHVFLTAQGPWALARAWLF
                     MPAQEPVSVILPDNAWEMGYGALELSDTLGICAIARRVGTEKAESRRYRTIIYPGGSV
                     EYVLWVDEFTGKWQDGLKKMFREHYLFDLETFDETLYQRKDLHWIRRSYLIGLQFAWN
                     REFYDWKEQRYTADSFLEKGQELFGGYDVIGLWPTWPRLGVDRRNQWDMFADLPGGLE
                     KIREFSVNWKQKGTKFFICYNPWDESTRTENPYQGMARLIEATDADGVVLDCHGASSH
                     ALQHAADSVKAGVIMYSEGMAVVKDMPGIVAGRVHDAIVMSPLLNLNKMIRPDFGIFR
                     VCQLHDGRIRREVSIALFNGYGTELNTFAPGQAANMENDLVYLGKTTQILRENTSCFT
                     SVAWTPLINTTYDGIWVNEWPEEKGKTLFTVLSLRPEGFSGPLFKYETGQGKHAISLW
                     HHEELDWIEKEGEIYIPAKLEAFNSFDIGTRAESSVDCIAILPELLNVKLESGILNII
                     AQAGDEIRIWAGNPSYQNKACRIFPPGIYRIDVTDIFDGFEGKIVVQLFDDHELADER
                     IVVLEPGSPRLVRETIIPGTDKNYTGDMIRIPGGTFYFRAENTDQFIPYPDTRDAVTR
                     EVTSFYIDKYPVTNQKFLEFLKDSGYRPSDTVNFLKHWQDGTIPKGLEDHPVVYVSLE
                     DARVYAEWAGKRLPTEVEWQYAAQDTDGRIWPWGSEFDSTRCNNGSGFTTAVDAFPLG
                     ASPFGVMDMTGNVWQLTGDVYDNGTYYFVIMKGGSFYKPTSSWWYVKGGPQPNTWHQQ
                     LLLVSPGFDRCATVGFRCVKDSGE"
     gene            complement(9828..10568)
                     /locus_tag="PKZ74_05510"
     CDS             complement(9828..10568)
                     /locus_tag="PKZ74_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92248.1"
                     /translation="MKSKAMFILAGFICLFFETGAQVDGIKTTVNPSSKFKQTDSPTA
                     RQDLAMLQDHNTTGGGAGAASFGGQSGGIYLEPGWAPGSVTLRNNKVIDNIMLRYDIY
                     HQQLQFIQDEDTLAFARPEEAEFFLIGGKKFIYAEYLNGAVIGKSYFEVLSEGRCELL
                     IRRVIRYHMNEESKSGQQDEAFIRECEFYLVKPGERAKPVATGRKSILCAFSDKEAEV
                     KNFMEEKNLKMKDCNQMKLVVDFYNSLQ"
     gene            10831..11337
                     /locus_tag="PKZ74_05515"
     CDS             10831..11337
                     /locus_tag="PKZ74_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPM92249.1"
                     /translation="MEWLLIVIGILLVLAGIAGAVLPGLPGPPLSFIALVLLQFRDEP
                     AFGEDFMILMGLIMIAVTVLDYVIPVYGAKKFGGTKKGVWGSTIGLVAGLFLLPALGI
                     VIGPFGLIGIIIFPFLGAYIGEKMSGRDSDTALKAAFGTFIGFLAGTVMKLVYSIVAA
                     VYFFIGIF"
BASE COUNT         3111 a         2717 c         2741 g         3165 t
ORIGIN      
        1 catttacatc tcatggtata tggcgacctg gtacaatcgc ctcaggtgct gctggctgaa
       61 aaactggctt cgtgcctccc ctctgccctt cggtccgtat atttcgttac atcgggcagc
      121 gaagccatcg aaggggcttt gaaactggca aagcgttata ccgggcggac cggtgtcgta
      181 gcgtttcata aggcatacca tggaggaacg cacggcgctt tgagcatttg cggtgatgaa
      241 aggctgaaaa cggccttccg gccgctgctt cccgacatta cccacattaa attcaacaga
      301 ttcgaagatc ttgaccaaat aacagacaaa accgcctgcg tggtggtgga gacgatccag
      361 gccgaagccg ggatcgtcct acccgccgcc ggttttctcg aagctttgcg cgcaaaatgc
      421 acagaaaccg gcgcattgct ggtaatagat gatatccaga tggggatggg acggaccggg
      481 aaattattca gctttgagca ttgcgggata gttccagata ttttatgcct ggccaaggct
      541 ttcggaggcg gcatgccgct gggtgcattt atctcgtcaa agtacatcat gcaaagcctt
      601 gcccatgatc ccgaactcgg gcacattact acgtttggcg gccacccggt gagctgtgct
      661 gctgcgctgg cctcgttgaa ttacctggta aatacaggca tatttgaagc agcggagcag
      721 aaagcccaaa tgttcatcga aagtcttgca ggtcatccgg ccattaagac tatccggcat
      781 aaagggttaa tgatcgggat cgacctggaa agcattgaga tgaatcaaaa actgatgaag
      841 gcattccatg aaaacggatt gattgccgat tcattccttt tccggccgca ggcctttagg
      901 atagcgcccc cgctgatcat aaccgaggaa gaaattgaaa tgacctgcga gaggatcatc
      961 gccagtttga atcgcctgta gaagcgaaga ttctttttac tgcatactta tttttcgata
     1021 tacttttcgg cagaatatcg actttttcga tgaacccatc cgggttattt tatacacctc
     1081 acattcagtc caaactcctt gtttgagttt tgtatctggc aatttgacgt gctgtaatgc
     1141 aacattttac tttgagtggt tgaattttgc gtactgctcc agcaatcgcc atccgtaccc
     1201 ctggcagcca ggttgcccgt agcgtcagtc aatacacctg ccatatgaag tttcaggaca
     1261 cttgcaaatg cttcattggc attatcccac tcaccattaa tatcagcgtt ggtccattcc
     1321 gtatttgtag gaagtcgcca acctgtcccc aacaataaat agcatggatc attttcaggc
     1381 tgccaatcat agttttcggt tatatcggtt atccatgccg tgctgggtgt ccgcacactg
     1441 ccatcatgtt tatatccctg ctgtcggtta aattgccagt accacccggc cggaggctca
     1501 gtggtctggt taacagtggt agcctggtaa tctgcgccca agttctgagt tatccagcac
     1561 tgttgtatac cgcttaagtc cgataagacg gttccgtacg tcacagtttt atcaacaggt
     1621 gctacagttc cagccacatg attcctgaca atcggggaac cacaagtttc attcggatta
     1681 atcgtttccg ttccataagg cacctctttc cataatcctg cactattcac ataaatataa
     1741 agtttctcat ccgttgtgca aaagaccatt aatcctgatg caggtccggg tattccttcg
     1801 atctgggcaa gtgtcatcct tggaggaaga aaccctttca catttgaact tatatctaac
     1861 aaagcagaac tatttggatt tgacgtccca ataccaatag attgagaacc gctgatataa
     1921 atcgcattgg caaaactgtt cggctctatg acgataacat ccatcaggtt cgcatgatcc
     1981 cggatcttga atccgcctga taaggttgcc ttgaatttcc agttagcacc gctggcattg
     2041 tcaagcatca catacgtcgc acctcccgaa ccccctaaat tctgtactgt aatcgtgggc
     2101 tcggtcatgc tttttgcgac atgaagaaga gtggccggag aattggtccc aatcccgaca
     2161 ttaccggaat tggtattgta gatgttgttt ccgttggcag cccattgtgc cagtgtttga
     2221 ccggctccgg aaaagatcag aaaaaaaatt cccgtgtaac gaaaaattgt tctcataatc
     2281 acttgggttt atggttaaca ataattgaat acatttcaga atcaatcatt tggatggtcg
     2341 tcgttaacca ataatcgtga cttaggcaaa gaagggatta ttgaataaat attcaccgtg
     2401 actaagataa gaaataaaaa tataaatgtc aaataattat agaaccatgc cattatgtct
     2461 ttttaaaatc tagatcattt gtaaatatca gccattatgg catatatttg actaattatt
     2521 ccttcggccc aaactttgat attttcaaat gcttaattta agtcattcat aattaagaat
     2581 taagaattaa aaattaaaca ttaacttaaa gtaaaggagg aacaagccat gttaccagca
     2641 ttaagaaaaa agagcacgtt tccgagtctg tttgatgagt tctttagcgg tgatgttttc
     2701 cccggttttt tccttgattt tgacaaaagg atggccacac ctgccgtcaa cattatcgaa
     2761 agcaaagatg attaccggat tgaggtcgcc gcgcccgggc tcgataaagc ggatttcagg
     2821 atcaatatcg aaaacaacat cctgacaatc agcgctgaaa aagaagaaaa acaggaagaa
     2881 aaagatgaaa aagtgatgcg ccgggagttc aattactcct cattttcgcg gtcgttcacg
     2941 ctgcccgaca gcatggatac tgaaaagatt tcagcccgac acaaggacgg cgtcctgaac
     3001 gtcattctcc cgaaaaaaga agaggccaag gaaaaaccgg ccaggaccat caaaatttcc
     3061 taaacacacc accgacggat ttacccgtcg gagtacaaaa caaataaccg acgggtaaat
     3121 ccgccggtta tttgttttgt acttttgaca tctcaaagaa acgtacgtgc cggtatattt
     3181 actcaactat gaaatgcgct ttcctgatcc caggctggct gatactgaag gattgctggc
     3241 tgttggcggt gacctgtcgc ccgaaaggtt gttactggca tacgagaagg gtatattccc
     3301 ctggttcagc gactattcgc cggtcttatg gtggtcgcct gacccgggat ggtgcttttt
     3361 ccaaaagagt tcaaaagatc aaaaagtctg cgtcagacta tgcgcagcag gcattttgaa
     3421 atccgattcg actcagactt cggcagtgtc atcacgaact gtgcttcaac ccggcgaaaa
     3481 catgaagacg gcacctggat cgttccggaa atcatcgatg cctattcgtg cctgcatgcg
     3541 aaagggtatg cccacagtgt ggaaacctac agggagggca aactggtcgg tgggttgtac
     3601 ggcgtgtcac tggggaaagc ttttttcggg gaatcgatgt tctacctcga aagagatgca
     3661 tcaaaagctg ccctggctgc cctggttgaa cgaatgactg aatgggactt tcattttatt
     3721 gatgcccagc aaaatacaga acatctgggc cggcttggcg cccggccatt gcaaagatcc
     3781 gtctttcttc accttctgca ggaagcggtc agctatccaa cggtcttagg gaaatgggag
     3841 taaataatat tacaagatga gtgacgaaca gaatcgggaa tttatggagg aggcgatccg
     3901 gctggccgca ttgaatatgg atgcgaccca cggcggccct ttcggatgtg tcattgtcaa
     3961 agacggccgg atcatcgctt cagcttataa tgaagtgctc cggaccaacg atccgactgc
     4021 ccatgcggag attgtggcta ttcgccgcgc ctgtgagaaa ctgaactcat atcagctggc
     4081 cggatgtgaa atctttacca gttgcgagcc ctgcccgatg tgcctgggtg cgatctactg
     4141 gagccgtccg gccaaggttt actatgccgc ttcaagaaaa gatgcggaaa aagccggttt
     4201 cgacgataac cacatttacc aggaagttgc cgttccagac caaaacaggg tcataccctt
     4261 cgagcagttc atgcccgaca aggtcgtaaa gctgtttcaa cagtgggttc agttaaaacg
     4321 tgacgtcaac tattaatgcc aggcaggaat tgaaccggac attcaaaacg cacatcgccc
     4381 tccttggagc caatttcatc tatggcctga attatgtcat cgctaaaggg atcatgcccg
     4441 atcacctttc cccaaaatcg atcattttca tccgggtcat cattacggtc gtgatattct
     4501 ggatccttca ccttttcata ccatctgaaa aagtagagaa aagggacttg ttcaaattcg
     4561 cattgtgcgc cttcttcggg gttgccgtca accagttgct gttcttcgaa ggattgaatt
     4621 tatccacccc tattaacgca tcgatcatca ttactgttat tccggtgctg gtcctgcttt
     4681 tttcacattt catactgcat gataaaatta ctccgatcaa agttgccgga attataatgg
     4741 gagctgccgg ggcattaatg gtcatccttt cagccggcgc cggcgatttc aggtcggcaa
     4801 ccatgctggg gaacctgctc atcttcatca atgcagcctc atggtcgttc taccttgtcc
     4861 tcataaaacc gctcatggag agatacgaca actttaccgt catgaaatgg gttttcctgt
     4921 tcggattcat catcatcttc ccattcacct tcaattcatt tgcctcgtcg tcattctcca
     4981 ctataccggt gaatatctgg ttatccatct cttttgtcgt atttggcgca accatcattg
     5041 cttattttct gaataattac agcctgaaat ccgttagccc cagcgttaat ggcatttata
     5101 tttaccttca accgctgatc gcatcggtgg tatccatcct ttttggcaaa gatcaaatct
     5161 cggtcatcaa gatattagct gccattctga tcatgacggg cgtttacctg gttacgcgcc
     5221 actcccgaaa aaccgcctcc attttccctg aacaataata ttttttgctc cgtcgggaaa
     5281 aagtctaatt ttgccaaaat ttttaaagat gaaaaaatat tatctctccg ccgtattatt
     5341 atttgcatta accttaagcg cttctgccca ggataccgta aaagtggccc tgaagacatc
     5401 cgccgactct ctttcattca gcttcggcta cctgatcggg aacaacttat ttgtccagaa
     5461 tgtcgaaaat atcaatcaga agattttcat gaaaggtttt aattatggat attcggacca
     5521 gaaaaccacc ttatcggtgg aagaagcaaa ccggatcatc cagcattatt ttcagaatat
     5581 gatctcagat gaatcaaaac agaacctcga aaagagcaat gcttttcttg aggaaaacaa
     5641 attgaaagag ggcgtcacca cccttccgag cggtctccaa tatacgatta tcacagcggg
     5701 tacgggcgaa tcgccaaaac cggaagataa agtaaaggtg cactataccg ggcatcttat
     5761 cgacgggact gttttcgaca gttcggtaga gcgcggcgaa ccgatcgtat tcggtgtaaa
     5821 ccaggttatt cccggatgga ccgaagcgtt gcaacttatg cagcccggag cacaatggat
     5881 tctttatatt ccgccagccc tggcttacgg tgaaaacggc gccggcggcg tcataggccc
     5941 gaaccaggcc ctcatcttcg aagtggaact gatagaggtg gttaaataaa gggtgtttct
     6001 tcctcaaaat caggtctata gctttcatgg cttgagaagt cctgcaccca gcctttactg
     6061 aaaccaagct tatggagggc ttcggttaca cgttgatact ccttttccga aatggtcctg
     6121 cccaatgatt catgattttg tacaaggtat gtgggataaa attgcgacat cagggaaata
     6181 tgcaactggt ttgacacctc gtatgcaatg gttctcaaaa cattgatgct gttcaccggg
     6241 tgtccgggca gcaccaggtg cctgacaatc accccgttcg tagcataacc cctgttatcc
     6301 accggcagac cggtcccttt ttgccttacc atttctttta acgcttccag ggcaacagta
     6361 gggtagttct ttacatcaga atattttctc cccaggtttt catccgaata tttataatca
     6421 ggaagataaa tatcgatgta accttccagg ccgcgaatgc tttcaacggt atcataacca
     6481 tttgtattat aaacaaatac aggatctgac cccatctcac gcaacgcatt gacaattact
     6541 ttgacatgcg ggataaaatg gcctggggat acaaacccga catggttaat ccctgttgct
     6601 agtatcgttg caatgcgttc aagaacctcc ggcaacgaca gcctgtttcc accgtaatcc
     6661 agtgaattgc agctgatctg atgattctgg caatagatac actgaagatt gcaattcgta
     6721 aaaaaaatat tgcaaatacc ccggtcgccc gaaatgaccg gttcttcacc tctatgcggg
     6781 aaaatggagg aaatggtgaa atcagtaccg gctttgcaat agcccctttt atccgaaaac
     6841 cggtcggcgt tgcaattccg cgggcaaatc ctgcaattct tcagttcctc aataggctta
     6901 accaattgtt caaaatcgag cctgctgata tgatcccgga tttccttcat cttgtgtccc
     6961 tgagtgcact atgtgtccga atagtaaatt tgataacagc aaagataaac ttggaaacat
     7021 cttcttaacc cattaaattt gtagaaacca aaaactgagt tatgcaccgg cttaatcttt
     7081 tactcgcatt tttctttatc tgcatccttc agactattca ttctcaggat attgcattat
     7141 cagtaaaaaa aggtgtaatc ctaaacagct atactttaaa tggcatgacg agtaaatttc
     7201 cggttactta tcctcttttg accttcaggt tagataacga attaatcagt cttgctgatg
     7261 caaaagccta ttgcactgat tcttccatta ttttcgatta caagggattg attaccggtg
     7321 agatcatcgc ggataatgaa tttcaagagg tctggcagcg ccgggtcatt ctacgcaatc
     7381 caaccgatga tacgatcgac attgaaaacc tggtcccttt cggcgaaatt cctgatcatg
     7441 tatttctgac tgcacagggt ccgtgggctc tggcaagggc atggcttttc atgccggcac
     7501 aggaacccgt cagcgttatc ctgcccgata atgcctggga aatgggttat ggagcgctgg
     7561 agctcagcga tacgcttggc atttgcgcca ttgcacgaag ggtcggcacc gaaaaagccg
     7621 aatccaggcg ttaccggact atcatttatc cgggtggatc ggtagaatat gttttatggg
     7681 tagatgagtt caccggtaaa tggcaagacg gattaaaaaa aatgttccgg gagcattatc
     7741 tgttcgatct tgaaacattc gacgaaacgc tgtaccaaag aaaagacctg cattggatcc
     7801 ggcgatcgta cctgatcggg ctgcaatttg cctggaaccg tgaattttac gattggaaag
     7861 agcaaagata cacagcggat tcatttctcg aaaaaggcca ggaattattt ggcggctatg
     7921 atgtgatcgg cttatggcct acctggccgc ggctgggagt tgaccggcgc aaccagtggg
     7981 acatgtttgc cgatttgccg ggaggattgg aaaagatcag ggagttctcg gtaaattgga
     8041 agcagaaagg gacgaagttt ttcatttgct ataatccctg ggatgagagc acacgaacgg
     8101 agaatccata ccaaggcatg gcgcggctga tcgaagcgac agatgccgac ggagtggtgc
     8161 tggattgcca cggcgcttcg agccatgcac tccagcatgc ggccgacagc gtgaaggccg
     8221 gcgtgatcat gtacagcgaa gggatggctg tggtgaaaga tatgccgggg atcgtcgccg
     8281 gccgggtgca tgacgccatt gtcatgtcgc cactgctcaa cctgaacaag atgatccgcc
     8341 ccgacttcgg cattttccgg gtatgccagc tgcacgacgg ccggatacgc cgtgaagttt
     8401 ccattgccct tttcaacggt tatggcaccg agttgaacac cttcgccccg gggcaagcgg
     8461 caaatatgga gaacgacctg gtctacctgg gaaagaccac gcaaatcctc agggaaaata
     8521 catcctgctt tacttctgtt gcctggacac ccctgatcaa caccacgtac gacggtatct
     8581 gggtaaacga atggccggaa gaaaaaggca aaaccctgtt taccgtgctg agcctgaggc
     8641 cggaaggatt ctccggcccg ttattcaaat atgaaacggg acagggtaaa catgccatca
     8701 gcctgtggca tcatgaggaa ctggactgga ttgagaaaga aggagaaatt tatattcctg
     8761 caaagctgga agcgttcaat tcgtttgata tcggaacgcg ggcagaaagc agcgtagact
     8821 gcattgctat tttgccggaa ttactgaatg tcaaactgga atcgggtatt ctgaatatca
     8881 ttgctcaggc tggcgatgaa atccgcatct gggctggtaa tccatcgtat cagaataaag
     8941 catgcagaat ttttccaccg gggatctacc ggatcgatgt gaccgatatt tttgacggct
     9001 ttgaaggaaa gattgttgtc cagttattcg atgaccatga actggcagat gaaaggattg
     9061 ttgtgctgga accggggagt ccccggcttg tgcgggaaac cataataccc ggaaccgata
     9121 agaattacac cggagatatg atccggatcc cgggtggaac gttttatttc cgtgctgaaa
     9181 ataccgatca gttcatcccc tatcctgata ccagggatgc ggtaaccagg gaggttacat
     9241 cgttttatat agataaatac cctgtgacaa accaaaagtt cctggaattt ctgaaggatt
     9301 ccggttaccg gccttccgat accgtcaatt tcctgaaaca ctggcaggat ggaacgattc
     9361 caaagggttt ggaggatcac cctgttgtgt atgtcagcct ggaggatgcc cgtgtctatg
     9421 cggaatgggc gggaaagaga ctcccgacag aagtcgagtg gcaatatgcc gcgcaggata
     9481 cggatgggcg gatctggccg tggggcagcg aatttgattc cacccgctgc aataatggct
     9541 ccgggttcac aacggcagtc gatgcctttc ctttgggcgc cagtcctttc ggggtgatgg
     9601 acatgacggg aaatgtgtgg cagctcaccg gtgatgttta cgacaacggc acctactatt
     9661 ttgtgattat gaaaggcggc agtttttata aaccaacatc gtcgtggtgg tacgtaaaag
     9721 gcgggccgca acccaatacc tggcaccagc agctcctgct cgtctcaccc ggattcgacc
     9781 gctgtgccac ggtcgggttc aggtgcgtga aagattccgg agaatgatta ctgcaaactg
     9841 ttataaaaat ccaccaccag tttcatctgg ttgcaatctt tcattttcaa attcttttcc
     9901 tccatgaagt tttttacttc tgcttcttta tcactgaaag cacaaaggat acttttccgt
     9961 cccgttgcaa ccggtttggc ccgctcgccg ggtttgacca ggtaaaactc acattcccgg
    10021 ataaaggctt catcctgctg gcccgacttt gattcctcat tcatatgata acggatcacc
    10081 ctccggatca acaattcaca ccggccttcc gagaggactt caaaatagct tttcccgata
    10141 actgctccgt taaggtattc cgcatagatg aatttctttc cgccgatcag gaagaactcc
    10201 gcttcttcag gcctggcaaa tgccagggta tcttcatcct gaatgaattg taactgctga
    10261 tgataaatat catatctcaa catgatatta tcgataacct tattattacg aagcgtgact
    10321 gaacccggag cccaaccggg ctcgaggtag atgccgccgg actggcctcc gaatgatgcg
    10381 gcaccggctc ccccgccggt agtattgtga tcctgcaaca tggccagatc ctgccgtgca
    10441 gtgggagaat ccgtctgttt aaattttgat gacgggttaa cggtggtttt aatcccatca
    10501 acctgggcac cggtttcaaa aaacaggcaa atgaaacccg ccagaatgaa catcgcttta
    10561 cttttcatgg ctcaatagat taaattactg attatcaata gatattatca gctctttctc
    10621 tataaagata atcatttacg agcctatttc aaagcatata tcgaaatatt ttatatatag
    10681 acgtaaggat gctattgatt attacatttt ttaacttttt ttatgaaatc atttttacaa
    10741 tgactcaatc aatcaggtac aatggcttca tttctgcttt aagcttgttt tcagggaatg
    10801 caatattatt tatactttta gaggacgatt atggaatggt tgctgattgt catcggaatt
    10861 ttattagtcc tggcgggcat tgccggggcg gtattgcccg gacttcccgg gccgccgctg
    10921 agtttcatcg cgctggtatt gcttcagttc agggatgagc ccgcattcgg agaggacttt
    10981 atgatcctta tgggattgat catgatcgct gttacggtat tggattatgt gatcccggtg
    11041 tatggcgcca aaaagttcgg cggaacgaaa aaaggggtat ggggaagcac gatcgggctg
    11101 gtggcaggcc tttttctttt gccggcactg gggatcgtta tcgggccatt tggactgatc
    11161 ggcataatca ttttcccttt cctgggagcc tatatcgggg agaagatgag cggcagagac
    11221 agcgatacgg ctttgaaagc ggcttttggc acatttatcg gtttcctggc cgggacagtt
    11281 atgaagctgg tatatagtat cgtagcggcg gtgtatttct ttatcgggat attttgattt
    11341 tgtgatatta tgagcttgtg attttgtgat attatgagct tgtgatattg tgagcttgtg
    11401 attttgtgaa ctttaaaaaa tagttttgtt aataaattct tttgggaaaa gcaggtgagc
    11461 tattgacaag ggcagattgg ttccttatat ttgtttcgcc tgaacatcac taacttacta
    11521 aaagaagaaa aaggatcaat ttagcagacg cttacgcggg catgttgaat ttttctgata
    11581 atccccccga actcctttac tacaagtgct aagtgcaaca gactgatctc tacttagtca
    11641 taacaatagt aatcattatg ccattaaaca agaaactcga gcgcaaattc cggaaaacat
    11701 acgggattga cctgttgccg gttggtatgg cgga
//
LOCUS       DAOPNH010000075        11712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_5785_length_11712_cov_5.836236, whole genome shotgun sequence.
ACCESSION   DAOPNH010000075 DAOPNH010000000
VERSION     DAOPNH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_11712_cov_5.836236"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKZ74_05520"
     CDS             <1..320
                     /locus_tag="PKZ74_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPM92250.1"
                     /translation="NIRENFIDLVFFRDNQPVLCNAYPYSGKEDILYFILNACEQLKL
                     RPGDLDIIVSGQFQPDSETARLLNRYVGKLKVADPIQLFEYNGILKQMAPHRYTDLFS
                     LAL"
     gene            317..850
                     /gene="rsmD"
                     /locus_tag="PKZ74_05525"
     CDS             317..850
                     /gene="rsmD"
                     /locus_tag="PKZ74_05525"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPM92251.1"
                     /translation="MRIIRGTHRGRKIVAPSNLPVRPTTDLAKESLFNILENHIYFED
                     IAVLDLYAGTGSISYEFASRGSKKVVAIDINPACVRFISGTATRLGFDTLKAVRSSSV
                     GFLNYTNARFDLIFADPPYDLEGIEEVIRLIFEKDLLADNGWLIVEHPAEKDFSQMPG
                     FERKRNYGKVNFSFFRK"
     gene            complement(904..1125)
                     /locus_tag="PKZ74_05530"
     CDS             complement(904..1125)
                     /locus_tag="PKZ74_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPM92252.1"
                     /translation="MYWTLELASKLEDAPWPCTKEELIDWCIRSGAPAEVVENLKELE
                     DEGDIYESIEDIWPDYPTKEDFFFHEDEY"
     gene            complement(1212..1772)
                     /locus_tag="PKZ74_05535"
     CDS             complement(1212..1772)
                     /locus_tag="PKZ74_05535"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPM92253.1"
                     /translation="MSRFKIYTRTGDKGQTSLIGGTRVPKFHPRIEAYGTIDELNSFI
                     GLIRDHDIDPVYKSVLVEIQDRLFTAESIIAMDPDAESQVRIPNLADKDIEFLESEID
                     RMNEALPELRSFILPGGHPVVSYCHVARCVCRRAERHVIRLAQAQPVDETVMMYLNRL
                     SDYLFVLARRLAKDFNAPETNWLPRH"
     gene            complement(1759..2466)
                     /locus_tag="PKZ74_05540"
     CDS             complement(1759..2466)
                     /locus_tag="PKZ74_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92254.1"
                     /translation="MNNEVIRLVDITRHFKVGLEVVQALRGVSTVIKKNEFVALMGAS
                     GSGKSTLMNLVGCLDTPTSGQYFLNGNDVSKLDDNELAEIRNKEIGFIFQTFNLLPRS
                     TALDNVMLPLIYAGIGKNDRMERAVKALEAVGLSDRMNHKPNELSGGQRQRVATARAL
                     VNNPSLILADEPTGNLDSRTSDEIMALLVDIHKAGNTIILVTHEESIARYAHRIIRLM
                     DGRILSDELNRDVYVKV"
     gene            complement(2502..3317)
                     /locus_tag="PKZ74_05545"
     CDS             complement(2502..3317)
                     /locus_tag="PKZ74_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM92255.1"
                     /translation="MNNSSTPGLWRTGRFLEFLFRSGTRYRVHSPFLYGLITEVIRKN
                     SNIPDERPILDLKKRLWSSSETILKTDYGSGSGKDGEEPGIYPVSLRKLVSGSVSSSR
                     KALRFYRLIRYSNFNSILEIGTSIGLTTAYFSKANPSARIITLEGCPELSRIAVENWK
                     KLGLGNISISVGRFEDTLAPALESLGKADFILVDGNHRKEATIRYFEICLPFMNNDSV
                     MVLDDIHASRGMEEAWEHIVTRPEVKVSLDLFYSGWLFFRKESSKEHFKLRYF"
     gene            complement(3310..4839)
                     /locus_tag="PKZ74_05550"
     CDS             complement(3310..4839)
                     /locus_tag="PKZ74_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92256.1"
                     /translation="MNKILLTALVILNLSALSQTGVVRLEFEAAINSDIYQLVPLDRQ
                     GFLVFYETSGMAGEGAKNWFFSCFGPDFKEKWKTNIPVILNAEYQHYIFHDSLVYLTF
                     YNPGKVRSGSENFQVTTFDLVNGVSYETKTTLPPDGKPVEFIVAGDKAIVGMNLKNDQ
                     AGVFSIILKTGYLNEFTFTWPDQNFIEDLAYDLRNDLIVCLVNNYLSRRQNKLYFAAL
                     STECAFRHDLEIAPVMAGKYLNTGRIVIQDSSRFLILGTYGNVSTKIPSQAEYFGLES
                     AGVYSTRVSGNSQEFMNYYNFAEFNNLRAGVSARDFYRLQKKKERESPEYSMNYELLV
                     HDALIRDSSVIMFLEAFYPEFRTVSDISYDHWGRPVTQSYTVFEGYRFFNALLSAYDA
                     DGQLMWDNSLEINSSPTRNLEKKAGYYFDGRPALIFNNDGARISYRICLENTELEPFS
                     KLDLETAEFGDKITAAGKNRMIHWFGYNFLAYGYQTIQNNLLQDKNERTVFYINKISL
                     E"
     gene            complement(4900..6813)
                     /gene="dnaK"
                     /locus_tag="PKZ74_05555"
     CDS             complement(4900..6813)
                     /gene="dnaK"
                     /locus_tag="PKZ74_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPM92257.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNSEGRRTTPSIVAFTD
                     NNERKVGDPAKRQAITNPKRTVYSIKRFMGETFDQVSTETGRVTYEVVRGDNNTPRVK
                     IDDRFYSPQEISAMVLQKMKKTAEDYLGQSVSEAVITVPAYFSDSQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKKNKDIKVAVFDLGGGTFDISILELGDGVFEVKSTNGN
                     THLGGDDFDQTVIDWLAEEFKADEGLDLRKDAMALQRLKEAAEKAKIELSSSTETEIN
                     LPYIMPVDGVPKHLVRKLSRAKFEQLIDKYVQMTIDPCRQALKDAGLNASQIDEVILV
                     GGSTRVPVIQKIVREFFGKEPSKGVNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETLGGVFTKLIESNTTIPTKKSEVFSTAADSQTSVEIHVLQGERPMAMQNKSIGR
                     FHLDGIPPAPRGIPQIEVTFDIDANGILHVAAKDKATGRSQQIRIEASSGLSQDEINK
                     MKQEAQENADSDKKARETVDKLNAADALIFQTEKQLKEYGDKIPPALKGDLESALEKL
                     KEAHKSKDLAAIDSAMANLNEKWQKASTEMYQNTQAGQQQTGPQDGPGAGGQKGNQGN
                     DGEVTDVDFEEVK"
     gene            complement(6916..7947)
                     /locus_tag="PKZ74_05560"
     CDS             complement(6916..7947)
                     /locus_tag="PKZ74_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92258.1"
                     /translation="MKKLATLRFFTIAFSKTRLFFVLLVSVLMISCEEDFEINAPYQD
                     ITIAYGLIDPSDDTIFVKINKAFLGDGDILEMARIEDSSNYINNLMAVIEEWDGGNLV
                     RTYQLDTITIKNKEEGTFYNPYQVVYFTPYEPVTSRLYRLKINVNNKEVTATTSLIND
                     FTVSKPSAGSKFIQFRKGTDSDVEWVSAKYGKRYEVLIRFKYKEVLFSSPDTVYKQVD
                     WFLATKKALDDRGGDDMSVAYRNDGFYTLIGDKIPYQDAAKESDVKARYTNDVDFMIA
                     VAATEYNTYMEVNEPTNSIVQDRPDYTNISNGLGIFSSRYRIVRTKKVHAETIEVIQT
                     DLPQLKFVY"
     gene            8077..8895
                     /gene="panB"
                     /locus_tag="PKZ74_05565"
     CDS             8077..8895
                     /gene="panB"
                     /locus_tag="PKZ74_05565"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPM92259.1"
                     /translation="MITDSRKYAKITTHVLQEMKLKGEKIAMLTAYDYSFAILLDEAG
                     IDVILVGDSASNVMAGHSTTLPITLDQMIYHASSVMRAVKRALVVVDLPFGTYQGNSK
                     EALHSAIRIMKESGANAVKMEGGKEVLESIDRILSAGIPVMGHLGLTPQSINKFGTYV
                     VRATDDSEAAKLKEDALLLEKAGCFAIVLEKIPSKLASEVTDQVKIPTIGIGAGPSVD
                     GQVLVLHDMLGINMKFSPRFLRRYHNLSDEIKASVNTYIDDVKSRDFPNEREQY"
     gene            8998..9702
                     /locus_tag="PKZ74_05570"
     CDS             8998..9702
                     /locus_tag="PKZ74_05570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM92260.1"
                     /translation="MTNQILYEDNHLLIVNKQPGDIVQGDKTGDEPLVETLKSYLKEK
                     YHKPGEVFLGVVHRLDRPVSGAVVFARTSKALARMNAMMQSREVKKVYWAVSPHPGSL
                     PHLGEGRGGGLLTHYLKRNEQQNKSYAYDLPGEGRQRAELLYRVIAQGDRYDLLEVEL
                     LTGRHHQIRAQLAAIGRPIKGDLKYGAPRSNPDGSISLHSRMVEFVHPVSKAPVSITA
                     PLPEEGIWKKLAGDIV"
     gene            9765..10742
                     /locus_tag="PKZ74_05575"
     CDS             9765..10742
                     /locus_tag="PKZ74_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPM92261.1"
                     /translation="MKPFIQHLVGAAAVVIIIAGLKFGAGPINQILIAFLLAMVITPL
                     PEWLIKKGVRKSLAILISLLVILGGGFLVSAMLASSVSNLVEAIPEYQEKLSVLYENL
                     QEFAAMQNIDFSDLVKKININPDKIIGFTGKVITGLTNFFSSSFIIAMLVAFMIIELI
                     NYSTDLKKGKREESVFIKWLTGLGGDLRKYVNITTLTGVITGVLNFFFLLILGVDFAF
                     LWAFLSFLMNFIPNIGFIISFIPPALIALIMLGWWQALVVFIGFWLINAIVENVIRPI
                     FMKESLNISLLTTFLSLLVWGWILGMPGAVLGVPLTMVVMKIYEGQKEG"
     gene            11114..>11712
                     /locus_tag="PKZ74_05580"
     CDS             11114..>11712
                     /locus_tag="PKZ74_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92262.1"
                     /translation="MKRLITLFSLIVCLAISTQVGAQSITNYTFTPSSGSFTALSGGT
                     TMALSGGTVDDGWFNGIPIGFSFVYMGIPYNTVSATTNGIISFGGNLTTSYAANGLAS
                     NGVRPIVAPLWDDLDLQVNTNFSYQTSGTTPNQVFTAEWLNVQWNYYATGNTISFQVK
                     LYEATGAVEFVYRDEGGTVNSGSASIGITAIATGSGNYLS"
BASE COUNT         2864 a         2811 c         2863 g         3174 t
ORIGIN      
        1 tgaatatcag ggagaatttc atcgatctgg tatttttccg ggataatcag ccggtgcttt
       61 gcaatgctta tccttattcg ggaaaagagg acatcctcta tttcatcctc aatgcctgcg
      121 aacagcttaa gttgcggccc ggcgacctgg acatcattgt atccggccag tttcaacccg
      181 attccgaaac agcccggctt ctgaacaggt atgtcggaaa actgaaagtg gcagatccta
      241 ttcagttatt tgaatataat ggaattctga agcaaatggc gccacaccgc tacacagacc
      301 ttttttcgct cgcattatga ggataatacg aggcacccac cggggacgaa agatcgtcgc
      361 gccgtcgaac ctgccggtca gaccgacaac cgacctggcc aaagaaagcc ttttcaacat
      421 cctggagaat catatttatt ttgaagatat cgctgtgctg gacctttatg ccggtaccgg
      481 cagcataagc tacgagtttg ccagtcgcgg aagtaaaaag gtggtagcca tcgatattaa
      541 tccggcttgt gtcaggttta tttccgggac agccaccagg ctgggcttcg acacccttaa
      601 ggcggtccgg tcttcttccg tcgggttcct gaactatacg aatgcccgct ttgacctgat
      661 cttcgccgat cctccctatg atctcgaagg aatcgaagag gtcatccggt tgatcttcga
      721 aaaagatttg ctggccgaca atggctggct tattgttgaa catccggcgg aaaaggactt
      781 ttcacaaatg ccgggatttg aacggaaacg aaattacggg aaagttaact tcagcttctt
      841 caggaaataa aaaaggctgc catatcgcaa ggcagccccc tttatccaat gaaaatttct
      901 tgatcagtat tcatcttcat ggaagaaaaa atcttccttt gtaggataat cgggccagat
      961 atcctctatg ctttcataga tatctccctc atcttccaac tccttgaggt tctcaaccac
     1021 ttcggcaggg gcgcctgaac ggatacacca gtcgatcaac tcttccttgg tgcacggcca
     1081 gggagcatct tccagctttg aagctaattc cagcgtccag tacataacaa ttcgattagg
     1141 attaattatc ctgcaaaaat atattttttt aaataaaagt caaggggatt taaaaagttg
     1201 gtataatatg tttaatgcct tggtaaccag tttgtttcgg gagcattaaa atcttttgcc
     1261 aggcgcctgg ccagcacaaa caggtaatcc gacagccggt tcaggtacat catcacggtt
     1321 tcatcaacgg gttgggcctg cgccaggcgg ataacatgcc gctcggcccg cctgcagaca
     1381 cagcgtgcaa catgacaata cgaaaccacc ggatgccccc ccggaagaat aaacgaccgt
     1441 aactcgggaa gtgcttcatt catccggtca atctcacttt ccaggaattc tatgtcttta
     1501 tccgccaggt tgggaatcct cacctgggat tcggcatccg ggtccatcgc aatgatcgac
     1561 tcagccgtga ataacctgtc ctgtatctca accagcaccg atttgtaaac gggatcaatg
     1621 tcgtgatcac ggatcagtcc gatgaaggag ttcagttcgt cgatcgtccc ataagcctca
     1681 atccggggat gaaactttgg aaccctcgta ccgccgatca gggaagtctg tcctttatca
     1741 ccggttcggg tatagatctt aaaccttgac atacacatcc ctgtttaact cgtccgacaa
     1801 aatccttcca tccatcagcc tgatgatgcg atgggcataa cgtgcaatgg attcctcatg
     1861 agtgaccagg atgatcgtat taccggcttt atggatatcc accagcaaag ccatgatttc
     1921 gtcagaagtc ctggaatcga ggtttccggt tggctcatcg gccaggatca gcgatgggtt
     1981 gttgaccagc gcacgggctg tggcaacgcg ctgcctttgc ccgcccgaca actcattggg
     2041 cttatgattc atacggtctg acaggccgac cgcctccaat gctttgacgg ccctctccat
     2101 ccggtcattt tttccaattc cggcatagat caacggcagc atcacattat caagcgccgt
     2161 ggaacggggc agcaggttga aagtctggaa aataaatccg atctccttgt tcctgatctc
     2221 ggccaactcg ttgtcgtcca atttactgac atcatttccg ttcaggaagt actggcctga
     2281 ggtaggcgta tcaaggcatc cgacaagatt catcagcgtt gatttccccg atccggaagc
     2341 acccatcagc gccacaaatt catttttctt aatgacggta gaaacacccc gcaacgcctg
     2401 gactacctcc agtccgactt taaaatggcg ggtgatgtcc accagcctga tcacttcatt
     2461 attcatcaca gccttttctt tcaaacaaat gtataaaata gttagaaata tctcagtttg
     2521 aaatgttcct tcgacgattc ctttctgaaa aagagccagc cgctgtagaa caggtccagg
     2581 ctgaccttta cctccggccg ggtgactata tgctcccagg cctcttccat tccgcgggaa
     2641 gcatgtatat catccaacac catgaccgaa tcattgttca taaaaggaag gcaaatttca
     2701 aaatatctta tggtcgcttc tttccggtga ttgccatcca caaggatgaa atcggctttc
     2761 ccaagcgatt ccagcgccgg agccagggta tcttcgaaac ggcccaccga aatgctgata
     2821 ttaccaagtc caagcttctt ccagttctct actgctatgc gactgagttc ggggcatcct
     2881 tcgagcgtga tgatcctggc tgaaggattg gctttggaaa aatatgccgt cgtcaatcca
     2941 atggaagtgc cgatttccag tatgctattg aaattgctgt acctgatcag gcggtaaaaa
     3001 cgcagcgctt tacgggaaga tgaaacagaa ccggatacca gctttcgaag gcttaccgga
     3061 tagatgcccg gttcttcgcc atctttgccg gaaccggaac cgtaatcggt cttgagaatt
     3121 gtttcactgc tgctccacag tcgttttttc aggtccagga taggcctttc atccggtata
     3181 tttgagtttt tacggatcac ttcagtgatt aatccgtaca ggaaagggga gtggacccgg
     3241 tagcgtgtgc ctgaccggaa cagaaactca aggaatctcc ccgttcgcca taaacccgga
     3301 gtggagctgt tattcaagtg atatcttgtt gatatagaat acggttcgct cgtttttatc
     3361 ctgaagcaaa ttattctgta tggtttgata tccataggcc aggaaattgt aaccgaacca
     3421 gtggatcatc cggttcttgc cggctgccgt tatcttgtcg ccgaattccg ctgtttccag
     3481 gtctagcttt gagaagggtt ccagttctgt gttttcaagg cagatccggt aggaaatgcg
     3541 ggccccgtcg ttgttaaaga tcagtgccgg cctgccgtcg aaataataac cggccttttt
     3601 ctccaggttc cgtgtcgggg atgaattgat ctccagacta ttatcccaca tcagttggcc
     3661 gtcggcatca tatgcgctca gtaaggcatt gaaaaaccgg tacccttcaa aaacagtata
     3721 actttgcgtg accggccttc cccagtgatc gtaggagatg tcgctgacgg tccggaattc
     3781 cgggtagaag gcttccaaga acataatgac ggatgaatcc cggatcaagg cgtcgtgcac
     3841 aagcaattcg taattcatag agtattccgg agattcccgc tcttttttct tctgcagccg
     3901 gtaaaaatca cgagcgctaa cccccgccct gaggttatta aactctgcga aattgtaata
     3961 attcatgaat tcctgagaat tgccggaaac ccgggtggaa taaaccccgg ccgattccag
     4021 gccgaaatat tcagcttgtg acgggatttt cgtggagaca tttccatagg tgcccaggat
     4081 caggaaacgt gaactgtctt gtattacgat acgtcccgta ttcaggtact ttcccgccat
     4141 taccggggct atttcaaggt catggcgaaa ggcacattca gttgacaggg cagcaaaata
     4201 gagcttgttt tgcctccgcg acaaatagtt gtttaccagg cagacgataa gatcattccg
     4261 gaggtcgtat gccaggtctt cgatgaaatt ctggtccggc caggtgaaag taaattcatt
     4321 tagataacct gttttcagga tgatagagaa gacgccggcc tggtcatttt tcaggttcat
     4381 cccgacaatt gctttatccc ccgcaacaat gaattcgacc ggttttccat cagggggaag
     4441 agtagttttt gtttcatacg acacaccgtt gaccaggtcg aaggtagtga cctggaagtt
     4501 ttccgatcct gatcggactt tcccggggtt gtaaaaggtg agataaacca gtgaatcgtg
     4561 gaaaatataa tgctggtatt ctgcattcag gataaccgga atattggttt tccatttttc
     4621 cttaaagtcg gggccaaagc agctaaaaaa ccagttcttg gctccctcgc ccgccatccc
     4681 gctggtttcg taaaaaacca gaaacccctg gcggtccagg ggtacaagct ggtaaatatc
     4741 gctgttgatt gccgcttcga attcaagcct gacaacaccg gtctggctaa gtgccgatag
     4801 gttaaggatg actaaagcgg taagcaggat cttattcatc gaaagtaaaa cgcagggtac
     4861 aaaggtaata ttatacaggt tttacctgcg tagaagaagt tatttaacct cttcgaagtc
     4921 gacgtcggtc acttccccgt cgttaccctg gttacctttc tgcccgccgg ctcccggtcc
     4981 gtcctgtggg ccggtttgct gttgtccggc ctgggtattc tggtacatct cggtgctggc
     5041 tttctgccat ttttcgttca ggttggccat agcgctgtcg atagccgcca ggtctttcga
     5101 tttgtgagcc tctttcagtt tttccagggc cgattccagg tcgcctttca aagcgggggg
     5161 aatcttatcg ccgtattctt tcagttgttt ttcggtctgg aagatcagag catcagccgc
     5221 attaagcttg tcaaccgttt cgcgggcctt tttgtcggaa tcggcgtttt cctgggcttc
     5281 ctgtttcatc ttgttgatct cgtcctggct cagccctgag gaagcttcga tacggatctg
     5341 ctgcgaacgg ccggtggctt tgtctttggc tgccacgtgc aggatgccgt tggcatcgat
     5401 atcaaaggtc acttcgatct gcgggatgcc gcgcggtgcc ggcgggatac catcgagatg
     5461 gaacctgccg atgctcttat tctgcatggc catcgggcgt tcgccctgaa gtacatggat
     5521 ctcgaccgat gtctggctgt cggctgcggt ggagaacacc tccgattttt tcgtcgggat
     5581 ggtggtattc gattcgatga gcttggtaaa tacgccaccg agtgtttcga tcccaagaga
     5641 aagcggcgtc acgtcgagca acagaacatc cttcacttca ccggtcagaa caccgccctg
     5701 gatagcggcg ccaatagcca cgacctcatc cgggttgacc cctttggagg gctctttacc
     5761 gaagaattcc ctgacgatct tctggatgac cggtacgcga gtggatccgc ccacgaggat
     5821 gacctcatcg atctgtgaag cgttcagccc ggcgtctttc agcgcctgcc ggcacggatc
     5881 gatcgtcatc tgtacatact tgtcgatcag ttgttcgaat tttgcccggg agagtttgcg
     5941 aaccaggtgc ttcggcacac cgtcgaccgg catgatatag ggcaggttga tctccgtttc
     6001 ggtggaactg gaaagctcga ttttagcttt ttcggctgct tctttcagcc tctggagcgc
     6061 catagcatcc ttccgcaggt caaggccttc atcggctttg aattcttccg ccagccagtc
     6121 gatcaccgtc tggtcgaaat catcaccgcc caggtgcgta ttaccattgg tcgattttac
     6181 ttcaaaaacg ccatccccca gctccaggat tgaaatatcg aatgtcccgc cgccaaggtc
     6241 aaacaccgcg actttgatgt ctttgttctt tttatcgaga ccgtaagcca gcgcagctgc
     6301 cgtcggttca ttgatgatac gacggacctt taatccggca atctcaccgg cttctttggt
     6361 ggcctgccgc tgtgaatcgc tgaaataggc cggaaccgta atgacagcct ccgacaccga
     6421 ttgtccaagg tagtcttctg ccgtcttttt cattttctgc agcaccatgg ccgaaatttc
     6481 ctgcggggaa taaaaacggt cgtctatttt aacgcgcggg gtgttgttat ctcccctgac
     6541 cacttcgtaa gtcacccgac cggtttcggt cgacacctgg tcaaaggttt cgcccatgaa
     6601 acgcttgatc gaataaacgg tacgtttcgg attggtgatc gcctggcgct ttgccggatc
     6661 acccactttc cgttcgttgt tatctgtaaa agcaacgatg gaaggcgttg tccttcttcc
     6721 ttcgctgttg ggaattacta ccggctcatt gccttccatg accgagacgc acgaattggt
     6781 cgtcccaagg tcaattccaa ttatcttacc cataaattat cagtattgtt aagaaattca
     6841 aaaatttcct ttggtttgtc aatgattatg ccatgattga ccttctgcca gaaagtgaca
     6901 aaaagtcatt ttaaatcagt aaacaaactt aagttgcgga aggtctgtct ggatcacttc
     6961 aatggtctca gcatggactt ttttggtgcg gacaattcga taacggcttg agaaaatccc
     7021 cagtccgttg gagatgttgg tgtaatccgg cctgtcctgc acaatgctgt tcgtcggttc
     7081 attgacctcc atgtaggtat tgtattcggt ggcggccacc gcgatcatga aatcgacatc
     7141 attcgtatag cgggctttaa cgtctgattc ttttgccgcg tcctggtaag ggatcttatc
     7201 tccgataagg gtataaaaac catcatttct gtaagcgaca ctcatatcat ctccgccccg
     7261 gtcatccagc gccttcttgg tggccaggaa ccagtcgacc tgcttgtaaa cggtatcagg
     7321 actgctgaat agaacttctt tatatttaaa cctaatcagc acttcataac gcttgccgta
     7381 tttggccgaa acccattcca cgtcgctgtc ggtgcctttc ctgaactgga tgaatttgga
     7441 accggcgctg ggtttactca cggtaaaatc attaatcagg gaagtagttg ctgtaacttc
     7501 tttattgttc acattgattt tcaaccggta aagcctggag gtgaccggtt cgtaaggtgt
     7561 aaaataaaca acctggtagg gattgtagaa tgtcccttct tctttgtttt ttatggtgat
     7621 ggtatctaac tgataagtcc tgaccaggtt tccgccgtcc cattcctcga ttactgccat
     7681 aaggttgttg atgtaattgc tcgaatcttc aattcgggcc atttccagga tatcgccgtc
     7741 gcccaggaag gctttgttga tcttaacgaa gatcgtatca tcggaagggt cgatcagtcc
     7801 atatgcaatg gtgatatcct ggtaaggggc attgatctca aaatcctctt cacaggaaat
     7861 catcaggacc gaaactaaaa gaacaaagaa aagtcttgtt ttgctgaatg caatagtgaa
     7921 gaaacggagt gtggcaagct ttttcatata attagtttta cggatggatg tccgctcgaa
     7981 aacaggatgc aaaattagct tattaaccaa taatttagcc ggctaatcga aatatttgtt
     8041 tttacctttg tatgctaacg ctgaaaacac ctcattatga taacagattc acgaaaatat
     8101 gccaagatca ccacgcatgt actgcaggag atgaagctga aaggggagaa gattgcgatg
     8161 ctgacggcct acgactattc ttttgccatt ttgctcgacg aagcggggat cgatgtgatc
     8221 ctggtgggcg actcggcctc caatgtgatg gccggccaca gcacaacatt accgatcacc
     8281 ctcgaccaga tgatatatca cgcctcttcg gtgatgcggg ctgtgaaacg cgctctggtg
     8341 gtggtcgacc ttccgtttgg cacctaccag ggcaactcga aggaggccct gcactcagcc
     8401 atccggatca tgaaagaatc gggcgccaat gccgtcaaga tggaaggggg aaaagaggtg
     8461 ctcgagtcga tcgaccggat cctgtcggcc ggtatccctg tgatggggca cctgggactg
     8521 acacctcaat cgatcaataa attcgggact tacgtggtca gggccaccga tgattcggaa
     8581 gcggccaagc tgaaggaaga tgccctcctg cttgagaaag cgggatgttt tgccatcgtg
     8641 ctggaaaaga tcccctcaaa gctggcatcc gaggtgaccg accaggtcaa aattccgacg
     8701 atcggtatcg gcgcgggccc gtcggtcgat ggtcaggtgc tcgttttgca cgatatgctt
     8761 gggatcaata tgaagttttc cccccgcttc ctccgccggt atcacaatct cagcgatgag
     8821 atcaaagcgt cggtcaatac ctatatcgat gatgtgaaat caagagattt cccgaatgag
     8881 agggagcagt attgagccaa tggtaattag ttcggcacta cgtacctcac gtcattagtc
     8941 attaatataa cgcgagcgca gcgagctaat gaccaatgac acgaacgaag tgagtaaatg
     9001 acaaaccaaa ttttatacga agacaatcat ctgctgatcg tcaacaagca gccgggtgat
     9061 attgtgcagg gtgataaaac cggggatgag ccgttggtcg agacgttaaa atcctatctg
     9121 aaagaaaaat atcataagcc gggagaggtg ttcctgggcg tggtgcaccg tctcgaccgg
     9181 ccggtcagcg gagcggtggt ttttgcccgg accagtaagg cgctggcccg gatgaatgcg
     9241 atgatgcaga gtcgggaggt gaagaaagtg tattgggctg tcagccccca ccctggctcc
     9301 ctcccccatt tgggggaggg acggggaggg ggcttgttga cccactacct gaaacgaaac
     9361 gagcagcaga acaaatccta cgcatacgat cttccgggcg aagggaggca gagggcagag
     9421 cttttatacc gtgtgattgc acaaggcgac cgttacgatt tgctcgaagt ggaactgctg
     9481 acgggccggc atcaccagat cagggcacag ctggctgcca tcggccgccc gatcaaaggc
     9541 gacctgaagt acggcgctcc ccgttccaat cccgacgggt cgatcagcct tcactcccgg
     9601 atggttgaat ttgtgcaccc ggtgagcaaa gcaccggtca gtatcacggc acctttgccg
     9661 gaggagggga tctggaagaa gttggcaggg gatattgttt gatttcatgt tccgttttct
     9721 atgaaataaa tgaataaatt cgtcagaaat taattctttt ccccatgaaa ccattcatac
     9781 agcatcttgt cggggcagcc gccgttgtta tcatcattgc cggtttaaag ttcggcgccg
     9841 gaccgatcaa ccagatcctg atcgcttttt tgctggcgat ggtcatcact ccgctacccg
     9901 aatggctcat aaaaaaaggc gtcaggaaaa gcctcgcgat tttaatctcg ctgctggtca
     9961 tccttggcgg gggattcctg gtttcggcca tgctggccag ttcggtcagc aacctggtgg
    10021 aggccattcc ggagtaccag gagaaactgt cggttctgta tgaaaacctg caggaatttg
    10081 ccgcaatgca gaacattgat ttctccgacc tggtcaaaaa gatcaatata aatcccgata
    10141 agatcatcgg atttaccgga aaagtaataa caggactgac gaacttcttc agctcttcct
    10201 ttatcatcgc catgctggtg gcattcatga tcatcgagct gatcaattat tcaaccgatc
    10261 tgaagaaagg aaaaagggaa gagtcggtat ttatcaagtg gctgacgggg ctggggggtg
    10321 atcttcgcaa gtatgtaaat atcactacct tgacgggtgt cattaccgga gtcctgaatt
    10381 ttttcttcct cctgatcctg ggggtagatt tcgcttttct gtgggcgttt ctatcctttc
    10441 tgatgaattt catcccgaac atcgggttta tcatctcgtt cataccgccg gccctgatcg
    10501 cactgatcat gcttggctgg tggcaggcgc tggtggtttt cattggtttc tggctaatca
    10561 atgccattgt ggagaacgtg atacggccca ttttcatgaa agagagcctg aacatctcat
    10621 tgctgaccac ttttctgtcg ttgctggtct ggggctggat cctggggatg ccgggagcgg
    10681 tgcttggggt acccttgacg atggtggtga tgaagatcta tgaaggtcag aaagaggggt
    10741 aaggaattga tattttgata ttttgatttt gtgatttttt gaacttatga ttttttgaac
    10801 ttatgatttt gtgattttat gaatttttga tcttttgagg ttttaatatt tcgatatttt
    10861 gatataatga tggcggggtg atgaaaatta attttgaatc attctaaatt agataattgg
    10921 ctgtgcgata tgtcgtattt aaccggaata aattatattt ttgataaata gtggaaaaag
    10981 cccgattcat gaaagccgga tcatgttttc ttgatttctt tttctgttgc ccttaaccaa
    11041 gggcattttt gccaatgtat aaaccatttc atgccaacaa caataaaaaa ataacctact
    11101 taaaacctta attatgaaac gactgattac acttttcagt ttgattgttt gcctggcgat
    11161 ttcgactcag gttggagctc aaagtattac aaactacacc ttcacaccat cttcgggttc
    11221 attcacggca ttgtcaggag gaactacgat ggcattgtct gggggaactg tggatgatgg
    11281 ttggtttaat ggtatcccta taggatttag ttttgtttat atggggattc catataatac
    11341 ggtttcagca acaacaaatg gaattatttc ttttggcggt aatttaacga cgtcatatgc
    11401 ggctaatgga cttgcatcga atggagtgag gccaatcgta gctccgctat gggatgatct
    11461 ggatttgcag gttaatacta atttcagcta ccagacttct ggaacaacac ctaaccaggt
    11521 attcactgct gaatggctaa atgttcaatg gaactattat gctacgggta atacaatatc
    11581 attccaggtg aaactttatg aagctactgg cgcggtagaa tttgtataca gggatgaagg
    11641 aggaaccgtt aatagcggca gcgcttccat tggtattact gccattgcca ccggatcggg
    11701 aaattacctt tc
//
LOCUS       DAOPNH010000076        11415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6021_length_11415_cov_5.742870, whole genome shotgun sequence.
ACCESSION   DAOPNH010000076 DAOPNH010000000
VERSION     DAOPNH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_11415_cov_5.742870"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PKZ74_05585"
     CDS             <1..1747
                     /locus_tag="PKZ74_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92263.1"
                     /translation="IDIDSYDFNTNSINYFDKNGVMHTKYYEGGELFSVDMFSADELL
                     NDNIASYVGYFGYDYKGNKLTTKPSFEDFFNAQDENGNYTREIGAYEPNYMAFYLQDK
                     FAFRDLIFNVGIRADRFDANQQMLKDPFLVYQAYTVKEVSSVGGDAVSHPSNMGSDYV
                     VYVDNVNNPTRVVGYRNEYDWFNASGAEIQDPNSLDVGSGISPYLVDPTLERPTIAAF
                     KDYEPQWSVMPRIAFSFPISDEALFFAHYDVLTQRPTNSVFSDPSTYYFFNNVSGTIN
                     NPGLKPTQTIDYEVGFQQKLTNSSSVKLVTFYREMRDMLQIYRFNGAYPKDYTSFNNI
                     DFGTVKGLTVQYDLRKTGNVRLSAYYTLQFANATGSNQGTTAALVASGLPNLRSTFPI
                     QTDRRHSFNIFLDYHFGGDAEYNGPVSQRKKSGKPPIQWLSYFGFTVTLNGGSGTPYT
                     QSRNVVGINQAGTNLVKGTYYGARGPWQFRTDATIDKDFHFMLGNGENQRPAFVNVYL
                     RINNILNQKNIMGVYPFTGNPDDDGYLSAPESQKQISEATSEQAYRDLYAIAVNNPGN
                     YSSPRTIRLGVIFNF"
     gene            1798..6099
                     /locus_tag="PKZ74_05590"
     CDS             1798..6099
                     /locus_tag="PKZ74_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92264.1"
                     /translation="MRIIYRLLSVVLIGLLLTGGAYSREFVGAKKNAGTIKGTAAGCS
                     AASGFRFLDINNIRARINTGGDMWWDLPGGIGAQYYIPKTGTATSMFSGSLWIGGLDI
                     NNQLKLAAIRYRQVGNDYWPGPLTTDGTAAVDQETCAKYDKHFLITRLEVDEFLGWWN
                     STNKAEEYPGYSIPKSILEWPAHGDVAKLNSYYLAPFRDVDGDGEYDPNNGDYPYYDI
                     TNELCPINFAGDPSYIPARTMEEDLTEYKVALTGSILVDQVIKGDQTLWWVFNDKGNT
                     HTETQGAPIGMEVRAQAFGFATNDEINNMSFYSYEVINRSTFELTNTYFCPWVDTDLG
                     YAWDDYVGCDVLRGLGYCYNGVAIDGSGEPESYGAQPPAIGVDFFQGPYIDPDGFDNP
                     RFTGDCSILGQGPNFEVDQMAINGVNFGNGIVDDERYGMRRFVYHNNTSSNPATTDPG
                     VASEYYNFLRGIWKDNSLMLYGGTAHNIDAAAVGPICEFMFPGDSDPCNWGTKFQPPN
                     GGYNQNGKYWTEEESGNEPGDRRFMQSAGPFTLKPGAVNYITVGIPWARAASGGPWAS
                     VELLRVVDDKCQALFDNCFKVIDGPDAPDLIIRELDRELIVFIDNPKGSNNYLEQYAE
                     FDPNIIQPNPANSELRSDSIYRFEGYQIFQLKDASVTLGDAKDEYGNFNPDMVRLVAQ
                     FDVKNGVGRIINHYFNKAIGFNIPVVEVEGGDEGIQHSFRITDDAFASGDKRLVNHKQ
                     YYYTVVAYAYNEYLPYDPEDVIGKMGQKKAYLPGRKNIVTYTAIPHKTINGLVMNSTY
                     GDGPAITRIEGQGNDGMILDLTPETIEEILSKQPVGTVIDGDTVDIGHPLYPIAYEAK
                     YVNGFGPLNIKIIDPLNVPTGKYYVRFDTLYTMRNFNVTGNTALIAGGDTASYITGSW
                     TLVDSLGREFKSDATLDKQNEQLFLDFGISINMKSFYTPGPKKVGQKPDQNGPITIYE
                     VLADNNGVLESSVTYQDSSYIWLGGVVDNDVPNDPRNWIRAGTYQDGNSSDWNMPSAS
                     FDPNQNFERLAGGTWAPFVLTAANDQDINGPAYSIACKERTFMEDVFSVDIVFTPDKS
                     KWSRSLVIEMCPDANLSQGKAARFQFRRAPSIDKDGNPASGGSGPSSDPNAANYVSDS
                     SMSWFPGYAINVETGERLNIVFGEDSWLVEDNGRDMKYNPTSSIFDPVSGNARFGGKH
                     YVYIMDHVRRDLQGRIFDFPAYDGCKYIRNGYGLNDLPPFLYELAWMSTAMWVGMPLS
                     IDGKEWLNNEVKIRIRVAKPYKRFYSTTLDSASIANSQNGHMPMYRFETQSVATQLSD
                     PEKAKSDLDLIQVVPNPYYAYAGGDGYERNALDNRIKITNLPERCTISIYNVNGTLIR
                     QITKDEPKTSVDWDLKNFAGVPIAGGVYIIHVKADNGEKILKWFGGLRVPDLNVF"
     gene            6141..7208
                     /locus_tag="PKZ74_05595"
     CDS             6141..7208
                     /locus_tag="PKZ74_05595"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPM92265.1"
                     /translation="MKNLYRYLAALTMITAFFFATSGVQAGNKDRSGQAGAPELLINP
                     WTGSAGWGGVNTSNIRGVESMFANIAGLAFVNKLELGFSQTYWLKGSGIGISNFGLGV
                     RAGETGAIGISVFSMRFGDLDVTTTEQPDGTGATYSPSLLNFALSYSKTFSNSIYAGL
                     TIRIISESIPDASAQGICLDAGVMYVTGERDQAKFGIALRNLGPTMKFTGDGFSIKAF
                     FQGNDNSISVIQRSESFELPTQLRIGASYDFQIAALHRLTLAGNFTSNSFSKDQFTLG
                     LEYSLNEILMLRGAYTYEDGILEDVSDPSRTNAYKGLSLGASVEVPLSKKTDLKLGID
                     YAYQSTDNFKGTQAFGIRIKY"
     gene            7302..7778
                     /gene="greA"
                     /locus_tag="PKZ74_05600"
     CDS             7302..7778
                     /gene="greA"
                     /locus_tag="PKZ74_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPM92266.1"
                     /translation="MAEPKYYTKEGLDKLRAELDHLTTVERPSISKQIAEARDKGDLS
                     ENAEYDAAKNAQGMLEMKIARLQEEISNARIIDESKMDLNKILILSTVKLSNLKNGAV
                     MSYTIVPESEANLKAGKISVSSPIAKGLLGKTVGDKVEIDVPAGKLNFEIIEISRS"
     gene            7789..8196
                     /locus_tag="PKZ74_05605"
     CDS             7789..8196
                     /locus_tag="PKZ74_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPM92267.1"
                     /translation="MPSIFTRIVNGEIPCYKVAEDDHFLAFLDINPLAKGHTLVIPKK
                     EVDYIFGVEDNMYAGLFLFAKRVALAVEKVVPCKRMGIAVLGLEVAHAHIHLVPINTV
                     YDIDFSKPKLVLQKEEFLELVEKISAEFSKGTQ"
     gene            complement(8190..10091)
                     /locus_tag="PKZ74_05610"
     CDS             complement(8190..10091)
                     /locus_tag="PKZ74_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPM92268.1"
                     /translation="MSEPIKHECGIAMIRLLKPFEHYLAKYGTSFYGLKKLHLMMQKQ
                     HNRGQDGAGIACVKFDLAPGHKFIERVRSNSGSAIQDIFETVYKRINAIGERDSRLLN
                     DAHWLKYHAEFTGELFLGHLRYSTFGKQGIELLHPVLRSNNWETKNLVLAGNFNLTNV
                     DELFERLIDLGQHPIETSDTITMLEKIGHFLDEENERLYREFKSSGHQKREISGLIGK
                     HLDLQAILTRSSKDWDGGYAIIGAIGHGDAFALRDPSGIRPAYYYKDDEVVVVASERP
                     VIQTSFNVPYESVREIKPGYALIIKKDGHVSEKKILEPLERKACSFERIYFSRGTDVE
                     IYRERQNLGKALAPAILDAIGHDLENTVFSFIPNTASVAFKGLMDEIEAYCAHTKKEK
                     IIQLGKDLSEESLEKILALRPRVEQVAVKDAKLRTFITQDKERDDLVGHIYDITYGSV
                     RKGVDSLVVVDDSIVRGTTLKQSIIRILDRLGPKKIIIASSAPQIRYPDCYGIDMSKL
                     HDFIAFHAAIHLLKETNQGNVINDVYKKCRAQLKLPKEQAVNHVKEIYKPFTATQISD
                     KIAAMLTTGETKAVIQVVYQNIEDLHAACPNHPGDWYFTGNYPTPGGNKAACKAFINY
                     IEGRDIRPY"
     gene            10223..11269
                     /locus_tag="PKZ74_05615"
     CDS             10223..11269
                     /locus_tag="PKZ74_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92269.1"
                     /translation="MNNLTIAETTIARETKALDAWHQGNPSPYKSSTAPNGKVYSPFS
                     FNIDPMERLILVNFEKDPDEFYTVFEFQQACDIMGRKHLLVIAYRRDDATDVYYQEGY
                     PFGSQDSVLNNACFFIRPLENARFEVNAGLIDVYFAFEDKMGREIIVQIKESKCRKKN
                     PFFLLAPVGANSEKPMSLPVYSLYQMSFLKQKHSVVEIEIDKVKHKPDTFPLPVDCAK
                     NYFTRYSADTFNVDWNKNFTGQLSPLIPDKVNIIEDKGIAYEMEETGGHYEIKRMSAR
                     NSKHHITIEFSPAFPDIICLREGTDIGGNFSIITDNTTGSIGGHYRIKDKKTKLTCKL
                     IPMEVGNPGRAGWF"
BASE COUNT         3029 a         2657 c         2869 g         2860 t
ORIGIN      
        1 tatcgatatc gactcctatg actttaatac aaactctatc aattatttcg ataagaatgg
       61 cgtaatgcac accaagtatt acgaaggagg agagttgttc tcggtggata tgttcagtgc
      121 agatgaattg ttgaatgaca acattgcttc ctatgttgga tattttggat atgattataa
      181 aggaaacaaa ctgacaacca aacccagctt tgaagatttc ttcaacgcac aggatgaaaa
      241 cggcaattat acccgtgaaa ttggagccta tgaaccaaac tacatggctt tctacctgca
      301 ggataaattc gcattccgcg atctgatctt taacgtggga atccgtgccg accgcttcga
      361 tgccaaccag cagatgctga aggatccgtt cctggtttac caggcctata cggtgaagga
      421 agtttcgtcg gtcggtggag atgccgtctc gcatccgtcc aatatgggaa gcgactatgt
      481 tgtttatgtc gataatgtta acaatcccac aagggtagtc ggctaccgca atgagtacga
      541 ctggttcaac gcctccggtg ctgaaatcca ggacccgaac agtcttgatg tcggctcagg
      601 gatctctcct tacctggtcg atcctacact ggagcggcct accattgctg cattcaaaga
      661 ttatgaaccc cagtggtcgg tcatgccgcg tatcgccttt tcattcccga tctccgatga
      721 agcgctgttc tttgcacatt atgatgtgct tacccagcgc ccgacaaaca gtgttttcag
      781 cgacccttcc acgtactact tctttaataa tgtatccggt acgatcaata acccgggttt
      841 gaaaccgaca cagactattg actatgaagt gggtttccag cagaaactga ccaattcatc
      901 atcggtgaaa ctggttacat tctatcgtga aatgcgcgac atgctccaga tctaccgttt
      961 caacggagcc tatcctaaag attatacctc ttttaataat atagactttg gaacggttaa
     1021 gggtcttacc gtgcagtatg acctgagaaa gaccggaaac gtcaggttga gcgcatatta
     1081 taccttgcag tttgccaatg ccaccggttc aaaccaggga acaacggcag cccttgtcgc
     1141 ctccggatta cctaacctca ggagtacctt cccgatccag accgaccgcc gccactcctt
     1201 caacattttc ctcgattacc attttggcgg ggatgctgaa tataatggcc cggtcagcca
     1261 gcgtaaaaaa tcaggtaaac ctcccattca gtggttgagc tatttcggat ttaccgttac
     1321 tcttaacgga ggatcgggaa cgccgtacac tcagtcgagg aatgtggtcg ggataaacca
     1381 ggcaggtacc aacctggtga aaggaaccta ctacggcgca cgcggcccct ggcagttcag
     1441 gaccgatgcc acgatcgaca aggatttcca ttttatgctg ggtaacgggg aaaaccaacg
     1501 ccctgccttt gtcaatgttt atctgcgtat caataatatc cttaaccaga aaaacatcat
     1561 gggcgtatat ccgttcacgg gcaatccgga tgatgacggt tatctgtcgg ctcctgaatc
     1621 ccagaagcag atcagtgaag caacaagtga gcaggcttac cgcgatttgt atgccattgc
     1681 agttaataac ccgggcaatt acagcagccc gcggacgatc aggctcgggg ttatcttcaa
     1741 cttttaatgc ttaggaacca ttcttcaatt gataatatta agaattataa tatgaaaatg
     1801 agaattatct atcggttact gtccgttgtg ctgatcggat tgctcctgac aggcggcgcc
     1861 tactccaggg aattcgtcgg ggcaaagaaa aatgccggta caattaaagg aacggcagcc
     1921 gggtgttcag ccgcttcagg ctttcgtttc ctggatataa acaatatcag ggctcgtatc
     1981 aacaccggcg gagatatgtg gtgggacctt ccgggaggaa tcggagccca atattatatt
     2041 ccgaaaaccg gaaccgctac ctccatgttt tccggatcac tctggatcgg aggactggat
     2101 atcaacaacc agcttaaact ggctgctatc cggtaccgcc aggtaggtaa cgattactgg
     2161 ccgggaccgc tgacaaccga cggaaccgcg gcggtggacc aggaaacctg tgcaaaatac
     2221 gacaagcatt tcctgatcac ccgactggaa gtagacgaat tcctcggatg gtggaacagc
     2281 acgaataaag cggaggagta tccgggctac agtattccca aatctattct ggaatggccg
     2341 gcccacggcg atgtagccaa actgaacagc tattacctgg ccccgttccg ggatgttgac
     2401 ggcgatggag aatatgaccc gaataacggc gactatccgt actatgacat taccaatgaa
     2461 ctttgcccca taaattttgc cggcgatccg agttatatcc cggcaaggac catggaagag
     2521 gatctgacgg aatataaagt tgcgcttaca ggcagtatcc tggttgacca ggtcatcaaa
     2581 ggcgaccaga cgctttggtg ggttttcaat gataaaggaa atacccatac ggaaacgcag
     2641 ggcgccccga tcggtatgga agtaagagcc caggccttcg gttttgcgac caacgatgaa
     2701 atcaacaata tgtcgttcta ttcctatgaa gtgatcaacc gttcaacctt tgaactgacc
     2761 aacacctatt tctgcccctg ggtagatacc gacctgggat atgcatggga tgactatgtg
     2821 ggttgtgatg ttctccgtgg attaggctat tgctataatg gcgttgcgat cgacggcagc
     2881 ggtgagccgg aatcgtacgg tgcgcagccc ccggctatcg gcgtcgactt tttccagggc
     2941 ccgtacatag atccggatgg attcgacaat cccagattta cgggagattg cagcatcctt
     3001 ggccagggac cgaattttga agtcgaccag atggccatca atggcgtaaa cttcggcaac
     3061 ggaatcgtgg atgacgaacg ttacgggatg cgccgttttg tttaccataa taatacctct
     3121 tccaacccgg caactactga cccgggagta gcttccgaat actataattt cttaaggggc
     3181 atatggaagg acaattcatt gatgttatac ggtggaaccg cgcataacat agatgcggct
     3241 gctgtgggac ctatttgcga attcatgttc ccgggcgatt ccgatccctg caactggggg
     3301 acgaaattcc agccaccaaa cggcggatat aaccagaacg ggaaatactg gacggaggaa
     3361 gaatcgggca atgagccggg agaccgccgt tttatgcagt cagccggacc atttacgctg
     3421 aaacccggcg ctgttaacta tatcacggtc ggtatcccct gggcaagggc agcatccggc
     3481 ggcccctggg cttccgttga actgctgcgg gttgtggatg acaaatgcca ggcactgttc
     3541 gacaactgct ttaaagtgat cgacggcccc gatgcaccgg accttattat ccgggaactt
     3601 gaccgggaac tgatcgtttt catcgataat cccaaaggct ccaacaatta ccttgaacaa
     3661 tacgccgaat tcgatccgaa tattatccag ccaaatccgg caaatagcga actcagaagc
     3721 gattccatat accggtttga aggatatcag atcttccagt tgaaagatgc ctccgtaacc
     3781 cttggagatg ctaaagatga atacggtaac tttaatccgg atatggtcag actggtggcc
     3841 cagttcgacg ttaaaaacgg tgtaggcagg atcatcaacc attattttaa caaagctatc
     3901 ggattcaata tccctgttgt tgaagtggaa ggaggcgatg aaggaattca gcactctttc
     3961 cggatcaccg acgacgcatt cgcatcaggc gacaagcggc ttgtcaatca caagcaatat
     4021 tattataccg ttgttgcata tgcttacaat gagtatttgc cttacgaccc ggaagatgtc
     4081 atcgggaaaa tgggccagaa aaaagcttac ctgcccggaa ggaaaaatat tgtcacctat
     4141 acggccattc cacataagac catcaacggc ctggtaatga attccacata cggcgatggc
     4201 ccggcgataa cccgtatcga aggacagggc aatgatggaa tgatcctcga cctcacaccg
     4261 gaaaccattg aggagatcct gagcaaacaa ccggttggaa ccgtgatcga cggcgatact
     4321 gtcgatatcg gccatccttt gtatccgatt gcttatgaag ccaaatatgt taatggattt
     4381 ggcccactca acattaaaat tatcgatccg ttgaatgttc ccacaggaaa gtattacgtc
     4441 aggtttgata ccctttacac catgcggaac ttcaatgtga cgggcaacac agctctaatt
     4501 gcgggaggag atacggcaag ctatattaca ggcagctgga ctctggtcga ctcgttgggc
     4561 agggaattca agtcggatgc cacccttgac aagcagaatg agcaattatt tctcgacttc
     4621 gggatctcga tcaacatgaa gtcgttctat accccggggc ccaagaaggt gggtcagaaa
     4681 ccggatcaga acggcccgat cacgatttac gaagtgctgg ccgataacaa cggtgtactt
     4741 gagtcgtcag tgacttacca ggacagctcc tatatctggc ttggtggagt cgtagataac
     4801 gacgtcccca atgatccgcg taactggata cgggccggaa cttaccagga tggtaattca
     4861 tccgactgga acatgccttc ggcttcattt gatcccaacc agaacttcga gcgcctggcc
     4921 ggcggaacgt gggccccgtt tgttctcaca gcagctaacg accaggatat aaatggaccg
     4981 gcttacagca ttgcctgtaa agaaaggaca tttatggagg atgttttcag tgtcgatatc
     5041 gtatttacac cggataaatc gaaatggtca aggtcattgg tcattgagat gtgccccgat
     5101 gctaacctgt cacagggaaa ggcggcacgg ttccagttca gaagggctcc gtccatcgat
     5161 aaagacggaa atccggcttc gggcggcagc ggccccagtt cagatcccaa tgcagcgaac
     5221 tacgtgtcag acagcagtat gagctggttc ccgggttatg ccatcaatgt ggaaaccggt
     5281 gaacgactga atattgtttt cggtgaagac agctggctgg tagaggacaa cggaagagat
     5341 atgaagtaca acccgacttc aagcattttt gatccagtat caggcaatgc ccgattcggc
     5401 ggtaagcact atgtgtatat catggatcat gtcagaaggg atctccaggg aagaatcttt
     5461 gatttcccgg catacgacgg ctgcaaatac atccggaacg gatatggttt gaacgacctg
     5521 ccgccgttcc tgtatgaact ggcgtggatg tccaccgcga tgtgggtcgg tatgccgcta
     5581 tccattgacg gaaaggaatg gctgaacaat gaagtgaaga tcaggatcag ggtagccaaa
     5641 ccctataagc gtttctactc gacaacattg gattctgctt ctattgccaa ttctcagaac
     5701 ggccacatgc cgatgtaccg gttcgaaacc caatcggtgg ctacacagtt gagcgatccc
     5761 gaaaaagcta aaagtgacct tgacctgatc caggttgtgc ccaacccgta ttatgcttac
     5821 gccggcggcg atggttatga gcggaatgcg ctcgacaata ggatcaagat caccaacctg
     5881 ccggaacgtt gcacgatttc aatctataac gtaaacggaa cgctgatccg gcagatcaca
     5941 aaggatgagc ccaaaacctc ggtagactgg gatctcaaga actttgccgg tgtaccgatc
     6001 gcaggcgggg tctacatcat ccatgttaaa gccgataatg gggagaaaat cctgaaatgg
     6061 ttcggcggac tgagggtacc cgatcttaat gtattttaat cagagaccag gataaaataa
     6121 caatattaat ttcatccact atgaagaatc tttacagata cctggcagcc ttgacgatga
     6181 ttacggcatt tttctttgcc acctcagggg tgcaggctgg aaataaagac cgatccgggc
     6241 aggcaggtgc gcctgaacta ctgatcaatc cctggacagg cagcgccggc tggggaggag
     6301 tgaatacctc caatatcagg ggcgttgaat cgatgttcgc aaacattgca ggactggcct
     6361 ttgtcaataa actcgagctt ggattttcac agacctactg gctgaaaggg tccggtatcg
     6421 gaatatcgaa cttcggactc ggcgtcaggg ccggtgaaac cggcgcaata gggatttcgg
     6481 tgttctccat gagatttggc gatctggacg taacaacgac cgaacagccc gatggaaccg
     6541 gtgctactta cagtcccagc ctgctgaatt ttgccctttc ttattcgaag actttttcta
     6601 acagcatata cgccggtctt acgatccgca tcatttcgga atctatcccg gatgccagcg
     6661 cacagggtat ttgccttgat gctggcgtta tgtatgtgac aggggaaaga gaccaggcca
     6721 aattcgggat tgctttgaga aatctcggtc cgacaatgaa gttcaccggc gacggattct
     6781 ccatcaaagc tttcttccag ggcaatgaca attcgatctc ggtcatccag cgctcagaat
     6841 catttgagtt gccaacacag cttcgcatcg gggcatcgta tgactttcag atcgcggccc
     6901 ttcaccggct gacactggca ggaaatttca cttccaattc attttcaaaa gaccagttta
     6961 ccctcggcct ggaatactcg ctgaatgaga tcctgatgtt gcgcggagcc tacacatatg
     7021 aagacggtat attggaagat gtgtctgatc caagccggac gaatgcatat aaaggcctta
     7081 gccttggcgc ttccgtagaa gtgccgctca gcaaaaagac cgatctgaaa ctcggcatcg
     7141 attatgctta tcagtcgacc gataatttca agggaacaca ggctttcggg atccgaatca
     7201 agtactaaac atttaaaatt ataccgtatc tttgtgcgtt caaagagacc cttttatgga
     7261 agggtctctt tgttattaaa acattttaca gggattgaat catggcagaa ccaaagtatt
     7321 atacaaaaga aggtcttgat aagctgcggg cagagcttga tcacctgaca acagtggaac
     7381 ggccttctat ctcaaaacag attgccgagg cgcgtgacaa aggagatctt tcggaaaatg
     7441 ccgagtacga tgcagctaag aacgcccagg gaatgcttga gatgaaaata gccaggctgc
     7501 aggaagagat aagcaacgcc cggatcatcg acgagtcgaa aatggatctg aataagattc
     7561 tgatcctgag tactgttaaa ctatcgaacc tgaagaacgg ggccgttatg tcgtatacca
     7621 ttgtgcctga atcggaagca aatttaaaag ccggaaaaat ttccgtatct tcgcctattg
     7681 ccaaagggct gctcggtaaa actgtcggtg acaaagtgga gatcgacgtg cccgccggta
     7741 aactgaactt cgagatcatc gaaatttcac gttcataaac attataccat gcccagtatc
     7801 tttacccgga tcgtcaacgg ggagattccc tgctacaagg tagctgaaga cgatcatttc
     7861 ctggctttcc tggatatcaa ccccctggcc aaagggcata cgctcgttat acccaaaaaa
     7921 gaggttgatt atatctttgg ggttgaagat aatatgtatg caggcttatt cctatttgca
     7981 aaaagggtag cccttgccgt ggaaaaagtc gtaccctgca aacgaatggg tatcgccgtt
     8041 ttaggccttg aagtggccca tgcccatatt catctcgttc ccatcaatac cgtgtacgat
     8101 atcgatttca gtaaaccgaa gctggtgcta caaaaagaag agtttcttga actggttgaa
     8161 aaaatcagtg cggaattctc caaaggcact caatagggcc tgatatctct gccttcgatg
     8221 taattgatga acgctttgca cgcggctttg tttccacccg gtgtaggata attgccggtg
     8281 aagtaccagt cgcccggatg gttcgggcag gcggcatgca aatcttctat gttctggtaa
     8341 acgacctgga tgacggcctt tgtttcaccg gtcgtcagca tagccgcaat tttatccgag
     8401 atctgggttg ctgtgaacgg tttgtatatc tcttttacgt gattgaccgc ctgttccttc
     8461 ggtaacttta gctgtgccct gcattttttg taaacatcgt tgattacatt gccctggttt
     8521 gtctctttca aaagatggat ggcagcatgg aaggcgatga aatcgtgaag tttcgacata
     8581 tcgatcccgt agcagtcggg gtatcggatc tgcggggcgg aagaagcaat gatgatcttt
     8641 tttggcccaa gccggtcaag gatgcggatg atgctctgct tcagtgtagt accgcggacg
     8701 atcgaatcgt cgaccaccac caggctatcg acgcctttcc ggaccgatcc gtaagtgatg
     8761 tcatagatgt gcccgaccag gtcgtcacgc tctttgtcct gggtgatgaa agtcctgagc
     8821 ttggcatctt ttacggccac ctgctcgacg cggggcctca aagccagaat cttttccagc
     8881 gattcctccg ataggtcctt tccgagttgt atgatcttct cttttttggt gtgggcacaa
     8941 taggcttcta tctcgtccat caggcctttg aaagcgacag aggcggtatt gggaatgaag
     9001 gagaagacgg tgttctcgag gtcgtggccg attgcgtcga ggatggccgg ggcgagggct
     9061 ttgccgaggt tctgccgttc gcggtagatt tcgacgtcgg ttccgcgtga gaaatatatc
     9121 cgctcgaagg agcaggcttt gcgttcgagc ggctccagga tcttcttctc cgaaacatgc
     9181 ccgtcttttt taattataag ggcatagccg ggttttattt ccctgaccga ttcgtagggc
     9241 acattaaagg aggtttgaat gacggggcgc tcagaggcca ccacaaccac ctcgtcgtct
     9301 ttgtagtagt aagcaggacg gatacctgac ggatcgcgca gggcaaacgc atcaccatgg
     9361 ccgatagcgc cgataatggc atagccgcca tcccagtcct tactcgaccg ggtcaggatc
     9421 gcctgcaaat cgaggtgctt accgatcagt cccgagattt ccctcttctg atggcctgac
     9481 gacttgaatt cgcggtacag ccgttcgttc tcttcgtcga ggaagtgtcc gatcttttcg
     9541 agcatggtga tcgtgtcgga tgtttcgatc ggatgctggc ccaggtcgat gaggcgttcg
     9601 aacagctcgt cgacgttggt gaggttgaaa ttgccggcca ggacaaggtt tttcgtctcc
     9661 cagttgttgc tccgcagcac cgggtggagc agctcgatgc cctgtttgcc gaaggtgctg
     9721 taacggaggt gtccgaggaa cagctcgccg gtgaattcgg cgtggtattt tagccagtgg
     9781 gcatcgttga gcaggcggga gtcgcgttcg ccgatggcgt tgatgcgttt atagaccgtt
     9841 tcgaagatat cctggatagc ggagccggaa ttggagcgga cccgttcgat aaatttatgg
     9901 ccgggggcca ggtcgaattt tacgcaggct attccggcgc cgtcctgtcc gcggttatgt
     9961 tgtttctgca tcatcaggtg cagttttttc aggccgtaga aagaggtgcc gtatttggcg
    10021 aggtaatgct caaacggctt gagcagccgg atcatggcaa tgccgcattc gtgtttgata
    10081 ggttcgctca tcggggtgct gatagctttg caaaggtagc agaatatttg aatggtgggt
    10141 aagcgttgta agggttgtaa gggtggatcc ttttgcaaat tggatcacta atttttaact
    10201 ttacatcctt aaataaaaca tcgtgaataa cctgactatt gcagaaacaa ccattgcccg
    10261 ggaaactaaa gctctcgatg cttggcacca gggtaatccg tctccctata aatcatccac
    10321 agccccgaac ggaaaagtat attcgccatt tagtttcaat attgatccta tggagcgtct
    10381 catactggtt aattttgaga aggatccgga cgagttttat actgtttttg aatttcagca
    10441 ggcctgcgat ataatgggca ggaagcattt attggtaatt gcttaccgaa gggatgacgc
    10501 cacagatgtt tattatcagg aaggttatcc ctttggatca caagatagtg ttttgaacaa
    10561 tgcctgtttc tttataagac ctcttgagaa tgctagattt gaagtaaatg caggtcttat
    10621 tgatgtttat tttgcttttg aagataaaat gggccgtgaa ataatcgtgc aaataaaaga
    10681 aagcaaatgt cgaaagaaga atcctttctt tttacttgca ccggtaggag caaactctga
    10741 aaaaccaatg tctttgcctg tttattcact atatcagatg tcatttttaa aacaaaaaca
    10801 ttccgtcgta gaaatagaaa tagataaagt aaaacataaa cctgatactt ttcccttacc
    10861 ggtagattgc gcaaaaaact acttcactcg ttattctgcc gatacattta atgttgactg
    10921 gaataagaat ttcacgggtc aattatctcc tttaatccct gacaaggtta atataataga
    10981 agacaaggga atagcctatg aaatggaaga gacaggcgga cattatgaaa taaagcgtat
    11041 gagcgccagg aacagtaaac atcatattac catagagttt agtccggctt ttcctgatat
    11101 tatttgcctc cgagaaggaa ccgatattgg aggaaacttt tcaatcatca cggataatac
    11161 aacgggcagc atcggtggac attaccggat caaagacaag aaaacgaaat taacctgcaa
    11221 attaatcccg atggaggttg gaaacccagg gagagcaggt tggttttgaa gatcttattc
    11281 tctgttgtca ggatattcag ggaatggcca atgagctatg tctggaatgc aaggatcata
    11341 ttggataata caggccagcc aatgatgcaa tcggggtgga aaagggttta agcaaactaa
    11401 tccctatatt tgtct
//
LOCUS       DAOPNH010000077        11409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6028_length_11409_cov_5.768628, whole genome shotgun sequence.
ACCESSION   DAOPNH010000077 DAOPNH010000000
VERSION     DAOPNH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_11409_cov_5.768628"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="PKZ74_05620"
     CDS             complement(<1..1474)
                     /locus_tag="PKZ74_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92270.1"
                     /translation="MPGRISYQRIIFATGALILLISLFSCSTKKNTFSRRVYHNLTAR
                     YNVYFNGKESLNEGSIQLMKGVKDNYTKVLPVFNFGTKQEAAQLNQGMDRAIQKASLT
                     IQRHSMEFDGGEKVRWIDDAYMMIGKAYFYKQEYFGARRTFNFVIQEYGYNDIKYTAM
                     IWLALTYNMTEEYEKAEPLLNLVTADVSKGIVPYDIVKILPQVYADLYLRREEYDKAV
                     DYIEEAIFYNPKRQLKTRMMFILAQIYQAKGDYFQASEWYTKVIKRNPDYDLAFQAKI
                     NLAKSYDTNSGDRKHVTKILTRMLKDDKNKDYKDQIYYALADVAMRSADTAAGIDYLR
                     LSVSSSTTNNYQKSTSSLRLADLYFNMPKYENAQAYYDTAMMALPNDYPNYEEIDRKT
                     KILTDLVKSLIVIQHQDSLQRLVNMSEAERNAVIDNIIAAYQEEQKRLAEQAQLDQAL
                     KVQEQAISFSGPAGSQGLPAGGKWYFYNQQTKDIGYAGCRN"
     gene            1674..2564
                     /locus_tag="PKZ74_05625"
     CDS             1674..2564
                     /locus_tag="PKZ74_05625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM92271.1"
                     /translation="MAEEVKKRDKWYHKLRNKYKLVILNDETYEEKLSFKLSRLNVFV
                     ATGTLAVLLIIITTIIIAFTPLREYIPGYTDVSLYEQLYAIEKLTDSLHNDARQKSLY
                     LENLKLVLSGKDTTIKTPLKVDTANTYQNISGQPSEADSEFREEFESQLVYGTSDPVK
                     STGRNRQGNRSDISSFTFFTPLRGIVTNKFNPTAKHYGVDIVSVQNEAIKATLDGVVI
                     FTGWTIETGYTISIQHENDLVSVYKHNSALLKDQGSFVKAGDAIAIIGSTGEYTTGPH
                     LHFELWYNGVPVNPTDFIKF"
     gene            2583..3905
                     /gene="rseP"
                     /locus_tag="PKZ74_05630"
     CDS             2583..3905
                     /gene="rseP"
                     /locus_tag="PKZ74_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPM92272.1"
                     /translation="MDIFIKISQLLLSLSILVVVHEFGHFIAAKIFRTRVEKFYLFFN
                     PWFSLFKIKFRETEYGLGWLPLGGYVKISGMIDESMDKEAMKQPPQPWEFRTKPAWQR
                     LIIMIGGVTVNVILAFVIYIGILAVWGEEYLPTSEVNKYGIVTDSIARDMGLQDGDKI
                     LAVDGKYVADFNKIPVVLILEEAQTIQVEREGQKKDIMVPDGVLGKLVKQKDPTLLTP
                     RFPLEIEGFATSSAAADAGIQAGDRIITVNGDPTIYYDQFVKAVKSRKGEMVEAVVVR
                     GTDTLTFRVQVSAEGMVGISTNPAKFFTTEKKDYTILQAIPAGFVKTIDGIANYLKQL
                     KLLFSPEIKAYESVGGFISIGSIFPATWDWQAFWRLTAFLSIMLAILNLLPIPALDGG
                     HVMFLVYEIVSGRKPSDKFMEYAQIVGMVLLFGLLIFANLNDVLRLFK"
     gene            complement(4098..5018)
                     /locus_tag="PKZ74_05635"
     CDS             complement(4098..5018)
                     /locus_tag="PKZ74_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPM92273.1"
                     /translation="MSFVTKEKLFSKKWFIAYSLIALGAFVMAAGFVLFITPYKIVPG
                     GVYGISIILHHLFDFPVGLTAICLDIPITIIGTRILGPRFGVKTVVGFTLTAVFVDTL
                     TYFWGDLPLVQDNGLLSSIYGGVLVGIGLGLIFKARATSGGSDVIAMIISKFNKMPPG
                     QMIILVDSIVVIAGLFSFMDWVIPLYSLLTIFITGRVMDVILQGISYDKTLFIISDKH
                     ELIRDKIINDINRGGTYIQGHGMFGGQEKTIIFTNVNRREMAILQEYIHHIDPMAFMT
                     VINANEVLGEGFRSRTSLALITVIKAIGSI"
     gene            5182..6165
                     /locus_tag="PKZ74_05640"
     CDS             5182..6165
                     /locus_tag="PKZ74_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM92274.1"
                     /translation="MNFSKIAKLNVLPVLVFLWLAFPAHSQQEPNFSQYMFYGLNLNP
                     AFAGTEPAVNITAANRMQWTGFGKENGDQVAPRTYFIAADLPVRVLKGGIGAVIMNDA
                     IGHENTISVKIGYANQRNMGFGKLGIGAQLEFNNRSIDFSKLDPAGEDPLLNQLSSEE
                     SEMLIDFSLGLLYRVPDNYYIGVSGLHLIQTKGKPLVESSEGSLRMKMDRTFMITGGY
                     EVNFPRNPDFQFVPSVIIESNIAKTRLDINAMLRYKDAVWGGVGYRLGESVIILLGVQ
                     YKDFRIGYSYDINANRLALPIFGGTHEIMVNYRFKLEIDRGRKSYKNTRFL"
     gene            6270..7649
                     /locus_tag="PKZ74_05645"
     CDS             6270..7649
                     /locus_tag="PKZ74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM92275.1"
                     /translation="MKRLPAFLLILALSGILISCGDTGNGELVGVSRKTRPFFQADPY
                     GMVFIPQGSFTMGVGDQDKSYGFIYEPKTVSVASFYMDETEITNDEYRQFVNWVKDSI
                     ALRKLGDIRPDEYLIQENPKTGEIYDPPFLNWSAEIDWESEEQDIRDALEEMYVPEHE
                     RYFRKKELDTRQLFYEYYWIDYVAAARKDWSEEANTEDGAFANRPQGRRDRSVYIRKE
                     RLNVYPDTLSWIYDYTYSFNDPQTKSYFWHPAYNFYPVVGINWKQARAFCIWRTELLN
                     SKMDPRKGEPGIVDFRLPTETEWEWAARGGYNNNPYPWGGPYTRNERGCFLANFKPLR
                     GNYIDDGGLRTVIVAHYPPNDFGLYDIAGNVSEWTNTAFDPSTYNFTWDMNPNYTYNA
                     KEDDPPALKRKVVRGGSWKDIAYFMQVSTRDYEYQDTAKSYLGFRCILPYLGRNKDDN
                     PARASRVYN"
     gene            7705..8727
                     /gene="gldL"
                     /locus_tag="PKZ74_05650"
     CDS             7705..8727
                     /gene="gldL"
                     /locus_tag="PKZ74_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPM92276.1"
                     /translation="MGLNTFVRRRGFRNFMSKLYGWGASVVILGALFKINHYPFANEM
                     LIVGLGTEAIIFFFSAFEPPYVEPDWSLVYPELGGLYHGDETAESVKKKPTQQLDDLL
                     KQSNIDQKLMDRLGDGLRKLSDNTSKLSEITDAAGVTNEYLTKVKGAAKSVENLSKSY
                     DVTAETLQKDVNASAGYSNSIKAAGEHAAQLSNVYKEASETLKGDINVTKEFTNSMKT
                     AMQSANSLAEQYTKSAEALSLSAKQLDFATVDGKSYTEKLHQISEKLAALNSVYELQL
                     QSNKDQMQSTAQVRDSMAQLLKTMKESADTMATYKDQMNLLTQRISSLNEVYGGMLTA
                     MSGKAK"
     gene            8780..10432
                     /locus_tag="PKZ74_05655"
     CDS             8780..10432
                     /locus_tag="PKZ74_05655"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HPM92277.1"
                     /translation="MAGYKETPRQKMIAMMYLVLTALLALNVSKDMLEAFLVVNESME
                     NTIEVFENKLGNLYMDFEKQYSLKPQKVGDYWEKAQKARKLSNELKNYVDHVIFDVVR
                     QSEGKDSVKLMQDAYEMRTMRDPYNPSKDIQVPLLNMNNVATKDKYDESTNYFINKGK
                     AEELKERIEKYKADMIALVPEQFRQNIQLGLETDGDYYNADGEKQTWELHHFYHTILA
                     ASVTILNKIKAEVQTAEFDITAELLSEIDISDFKFDKIEAKVIPTTNYVLQGNKYEAE
                     VLVAAFDTRQTPEVFVLQGAEEITVSNKDRAQKVAGEQGVVKLSFPSGNVGPQKYAGV
                     VEVVSPEGERIPYNFSGEYVVAPPSLTVAATKMNVFYIGVDNPVSISVPGIADANLKP
                     SISTGTLTRDPEGKDWIVKVPQGQPKAIISVEALYQGETVPMGSKEFRVRRVPDPRAE
                     IAGQVEGPIDKNTLLAAGAIIPVMKDFEFDLYFEVKSFRLTTIIGGDGISKRANNNRL
                     SDEMISMIRSARSGQKFFFENITAEGPDKTPRSLNPITLEIK"
     gene            10449..11297
                     /gene="gldN"
                     /locus_tag="PKZ74_05660"
     CDS             10449..11297
                     /gene="gldN"
                     /locus_tag="PKZ74_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPM92278.1"
                     /translation="MKNIALLFISLIILAGISVPAKAQVLSDPPKDVINFDNELLDVK
                     PVPLPAIRQADMMWSKRIWREIDMRQKINQPFYYPIEPHDNWRNFISVIMDALKEGTI
                     TAYDISNTDEFTIPLNYQEIISRQIDTMHKTFQRPYPPYEEYDTVIYSEFNPSKVTRI
                     RIKEDWYFDKKHSQMLVRILGICPVMTKERNNEEYNEALFWIYFPEARPVLAHAQVFN
                     RFNDASRRTYDEIFIKRLFNSYIYKEQNVYDRRIQEYAQGLDALLEAERIKMELLNFE
                     QSLWEY"
BASE COUNT         3199 a         2677 c         2649 g         2884 t
ORIGIN      
        1 tatttctaca accggcatac ccgatatctt tggtctgctg gttgtagaaa taccatttgc
       61 cgcctgcagg taatccctgt gatccggctg gcccgctgaa cgaaatggcc tgctcctgca
      121 ccttcagcgc ctggtcgagt tgtgcctgct cggccagccg tttctgttcc tcctggtagg
      181 ctgcgataat attgtcgatc accgcattgc gctcggcctc gctcatattt accaaccgct
      241 gcaaactgtc ctggtgctga atgacaatca ggctcttgac caggtcggtc aggatttttg
      301 tcttccggtc gatttcctca tagttgggat aatcgttcgg cagtgccatc atggccgtat
      361 cgtaatatgc ctgcgcattt tcatatttag gcatattaaa ataaagatcg gcaagccgta
      421 atgaggaagt cgacttctgg taattattcg tggtggatga tgaaaccgac aaccgcaggt
      481 agtcgatgcc ggccgccgta tcggccgaac gcatcgccac atcggccaga gcataataaa
      541 tctggtcttt gtaatccttg ttcttatcgt ccttcagcat cctggtaagg atcttcgtca
      601 catgtttccg gtcgccgctg ttggtgtcgt acgacttggc caggttgatc ttggcctgga
      661 atgccaggtc gtaatcggga ttccgtttga tcactttcgt gtaccactcc gaagcctgga
      721 aataatcccc tttcgcctgg tagatctgcg ccaggatgaa catcatccgg gtctttaact
      781 gccttttggg attgtaaaaa atagcctcct caatgtagtc aacagctttg tcatactctt
      841 cacgccggag atacagatcg gcatacacct gcggaaggat cttcacgata tcgtagggaa
      901 caattccttt gctgacatcg gccgtcacca ggttcagcaa aggttcggct ttctcatatt
      961 cctccgtcat gttgtaggtc agcgcaagcc agatcatggc cgtgtatttg atatcgttgt
     1021 acccgtattc ctggatgaca aaattgaagg ttctccgcgc gccgaaatac tcctgcttgt
     1081 aaaaataagc cttaccgatc atcatatagg catcgtcgat ccacctgact ttttcacccc
     1141 cgtcgaattc catggaatgc cgctgtatgg tcagcgatgc tttctgaatc gcccggtcca
     1201 tgccctggtt cagttgagca gcctcctgtt tggtgccgaa attgaatacc ggcaacacct
     1261 tggtataatt atctttcacc cctttcatca actggatgga tccttcgttc agactctcct
     1321 tgccgttaaa atagacattg taccgcgctg ttaaattgtg gtaaacacgg cggctgaatg
     1381 tgttcttctt ggtagaacat gaaaaaaggg aaatcagaag aatcaacgct ccggtcgcaa
     1441 agatgatcct ttggtatgaa attcggcctg gcactttcct gtatagtatc ggttgctttt
     1501 aaacctgatt tttaaaaatt ttccgggtga tgcaataccc ggattaataa ctgagttttt
     1561 aaagaattat tatatgcaga cgattaactt ataatatact acagccgtgc aaaagtaaaa
     1621 aatattccct ttcggcgaaa aattccgata tttgccggct gaacgacagc agaatggctg
     1681 aagaagttaa gaaacgggac aaatggtatc acaagctcag gaacaagtat aagctggtaa
     1741 tcctgaacga tgaaacctat gaagaaaagc tgtcgttcaa gctcagccgg ctgaatgttt
     1801 tcgtggctac cggcaccctg gccgtcctgc tcattatcat caccaccatc atcatcgcct
     1861 ttaccccgct gcgcgaatat attccgggat ataccgatgt atcgctgtat gagcagctat
     1921 atgccattga aaaactgacc gattcgctcc acaacgatgc gcggcagaaa tctctctacc
     1981 tggaaaacct gaaacttgtg ctctccggaa aggatactac cattaaaacc ccgctcaaag
     2041 tcgataccgc caatacttat cagaatattt ccggacagcc ctccgaggct gattcggaat
     2101 tcagggagga gtttgaatcc cagctggttt atggtacaag tgatccggtg aagtcgaccg
     2161 gtcgaaaccg ccagggcaac cggtccgata tcagcagctt tacttttttt actcctttga
     2221 gagggatcgt gaccaataaa tttaatccga ccgctaaaca ttatggggtg gatattgttt
     2281 cagtacaaaa tgaagccatt aaagccacgc tcgacggagt ggtgatcttt accggctgga
     2341 ctattgaaac gggatacacc atcagcatcc agcatgaaaa cgaccttgtt tcagtatata
     2401 aacataattc tgccctgttg aaagaccagg gaagttttgt caaagccggt gatgccatcg
     2461 ccattattgg cagcacggga gagtatacta ccggtccaca ccttcatttt gaactatggt
     2521 ataacggtgt gccggtcaac ccgaccgact tcatcaaatt ttaactgcaa accaaatcat
     2581 tcatggatat ttttatcaag atctcccagt tgctgctcag cctgtcaata ttggtcgttg
     2641 tgcacgaatt cggccatttt atcgcggcta agattttcag gacccgggtg gaaaagtttt
     2701 accttttctt taatccatgg ttctccctgt ttaaaataaa gttcagggaa acggaatacg
     2761 gactgggatg gctcccgctg gggggatatg tcaaaatctc cggaatgatc gacgagtcga
     2821 tggataaaga agccatgaaa caaccgcccc agccctggga attccgcacc aagccggcct
     2881 ggcagagact gatcatcatg atcggaggtg taacggtgaa tgttatcctc gcttttgtta
     2941 tctatatcgg catactggca gtgtggggcg aagaatacct tcccacctcc gaagtaaata
     3001 aatatggtat cgttaccgat tctatcgccc gggatatggg attgcaggat ggtgataaga
     3061 tcctggccgt tgacggaaaa tatgtagcgg atttcaataa aatccctgtc gtactcattc
     3121 tggaagaagc acagaccatc caggtggaga gggaagggca aaagaaggat attatggtcc
     3181 ctgatggagt gcttggtaaa ttggtaaaac aaaaagaccc gacactcctg acgccgcgtt
     3241 tcccgcttga aatagaaggt tttgccacat cttcggctgc tgccgatgca ggtattcagg
     3301 ccggcgaccg gattattacg gtcaacggcg acccgacgat ttattacgac cagtttgtaa
     3361 aagctgtcaa atcccggaaa ggcgaaatgg tcgaagcagt ggtagtccgc ggtaccgata
     3421 cgctcacttt ccgggtacag gtatccgcag agggtatggt tggcatttcc accaacccgg
     3481 ctaagttttt cacgaccgaa aagaaagatt atactattct tcaggctatt cctgccggtt
     3541 ttgtgaaaac catcgacgga atagctaatt acctgaaaca attaaagttg ttgttctcgc
     3601 cggaaatcaa agcgtatgaa tctgtcggcg ggtttatctc catcggaagc attttcccgg
     3661 ctacatggga ttggcaggct ttctggcggc ttaccgcatt cctgtccatc atgctggcca
     3721 tcctcaacct tttgcccatt cccgccctcg acggcggcca cgtcatgttc ctggtatacg
     3781 aaatcgtcag cggacgaaaa cccagcgaca aattcatgga atatgcccag attgtcggta
     3841 tggtattact tttcggattg ctgatattcg ccaacctgaa cgacgtgctc cggctcttta
     3901 aatagtgaat ggtgaagggt gaagggtgaa gggtgaaggg tgaacggtga agggtgaacg
     3961 gtgaagggtg aacggtgaac cagaattcaa aacccggaac ctggaacccg gaacccggaa
     4021 cccggaattt aatttctaat ccgaactgat cttctctttc agcgaccgga atccctcccc
     4081 gagaacctca ttggcattca tatcgatcct atggccttta tgaccgttat caatgccaat
     4141 gaggttctcg accggaatcc ctccccgaga acctcattgg cattgataac ggtcataaag
     4201 gccataggat cgatatgatg gatgtattcc tggaggattg ccatctcccg gcggttaaca
     4261 ttggtgaata ttatggtctt ttcctggccc ccgaacattc cgtgaccctg gatgtaagtc
     4321 cctccccggt tgatatcgtt gatgatctta tcccggatca attcatgctt gtctgatatg
     4381 atgaacaggg ttttatcata gctgatccct tgcagtatga catccatcac cctgcccgta
     4441 atgaaaatgg tcagcagcga atacaggggg ataacccagt ccataaaaga gaaaagaccg
     4501 gcaatcacga caatcgaatc gaccaggatg atcatttgcc cgggaggcat cttgttgaat
     4561 tttgagatga tcatggcgat gacgtccgat ccgccggagg tggcccgcgc tttaaaaatc
     4621 aaacccaggc caatcccgac cagcactccg ccataaatgg aggaaagcag gccgttgtcc
     4681 tgcaccaggg gcaggtcacc ccagaaataa gtcagtgtat cgacaaagac ggctgtcagt
     4741 gtgaacccga cgaccgtttt aaccccgaat ctcggcccca ggatccgtgt cccgatgatt
     4801 gtaatcggaa tatcgaggca aatggccgtc aacccgaccg ggaaatcaaa caggtggtgc
     4861 aggatgatgg agataccata aacccctccc ggcacaatct tgtaaggagt gataaacaaa
     4921 acaaacccgg cagccatgac aaaagccccc agggcgatca gcgagtaggc tatgaaccat
     4981 tttttgctga atagtttttc tttggtaaca aacgacatat catttagatt tatatccgat
     5041 ggccaaaaat agggataatc attcctcgat tttgttaccc ggatatcact ttgtaaaata
     5101 ttcgtaaaat atcaccgttt aagacttcgg tgaaaaattt tcttaattta gcgttttatt
     5161 ttactaaccc gtttcctgaa gatgaatttt agcaagattg ctaaattgaa tgttctgcca
     5221 gtacttgttt ttctgtggct tgcctttccg gcacacagcc agcaggaacc gaatttttca
     5281 caatacatgt tttatggcct gaacctgaac cccgcatttg ccggaacgga acccgcagtg
     5341 aatatcacag cagcaaaccg gatgcagtgg acgggatttg gcaaagaaaa cggggaccag
     5401 gtggcgccgc gtacctattt tattgcggcc gatctgccgg tccgcgtgtt aaaaggaggt
     5461 atcggagccg ttattatgaa tgatgccatc gggcatgaaa atacgatttc ggtaaagatc
     5521 ggctatgcca accagcggaa tatgggattc ggcaaactgg gcatcggggc tcagctagag
     5581 tttaacaacc gcagcatcga cttcagcaaa ctggatccgg ccggcgaaga tccattgctc
     5641 aatcagcttt catcagagga aagtgaaatg ctgatcgact tctccctggg cttgttgtac
     5701 cgggttcccg ataactacta tatcggagtt tccgggcttc atcttatcca aacaaaaggg
     5761 aaaccccttg tcgaatcttc ggaagggagc ctgagaatga aaatggaccg gacgttcatg
     5821 atcacgggcg gatatgaggt gaattttccc cgtaaccccg actttcaatt cgtcccttcg
     5881 gttatcatcg aatccaatat tgcaaaaacc cggctggata tcaacgccat gctccgttat
     5941 aaagacgctg tctggggggg agttggctac aggctcggcg aatcggtcat catcctgctt
     6001 ggtgtccagt ataaggactt ccggatcggt tattcttacg atatcaatgc caaccgttta
     6061 gcgctgccca tttttggagg cactcacgaa atcatggtta attatcgctt taaactggaa
     6121 attgacaggg gcaggaaaag ttataagaat accagattct tataatcatt gaaaaaaata
     6181 gttgttttgt tttaatattt tttatttttg ggcacttttt aaaataatcc tatacgatgt
     6241 tcgttaatac ttggtcaaaa aaattcgtta tgaaaagact tccggcattt ttgctgatcc
     6301 tcgcattatc cggcatttta atcagttgtg gtgataccgg aaacggagaa ctggtgggtg
     6361 tcagccgaaa aacaagacca ttctttcagg ccgatcctta tggaatggta tttataccgc
     6421 agggtagctt cacaatgggt gttggcgacc aggacaaatc atatggattc atttatgaac
     6481 ccaaaaccgt ttcggtagcc tcgttttata tggatgaaac cgaaatcaca aacgacgagt
     6541 accgtcagtt tgtcaattgg gtcaaggatt ccatcgccct gcgcaaactg ggcgatatcc
     6601 ggccggatga ataccttatt caggaaaatc ccaagacggg cgaaatctac gatccgccgt
     6661 ttttaaactg gtcggcagaa atagattggg aaagcgaaga gcaggatatc cgcgatgcac
     6721 ttgaagaaat gtatgtcccg gaacatgaac gatatttccg taaaaaagaa ctggataccc
     6781 ggcaattgtt ttatgagtat tactggatag attatgtggc tgccgcccgt aaagactggt
     6841 cggaagaagc caacaccgaa gatggagctt ttgccaacag gccgcagggc aggcgcgacc
     6901 gctcggtcta tataaggaaa gaaaggctga atgtttatcc cgataccctg agctggattt
     6961 acgattatac atactctttc aacgatcctc agactaaatc ctatttctgg catccggcat
     7021 acaattttta cccggtcgtt ggtataaact ggaaacaggc cagggcattc tgtatctggc
     7081 gtaccgagtt gcttaacagc aaaatggacc cgagaaaggg tgaacccggt attgtcgatt
     7141 tccggcttcc cacagaaaca gaatgggaat gggcagcccg gggaggctat aacaataatc
     7201 cttacccgtg gggaggccct tatacccgta atgaaagggg ctgtttcctg gctaatttca
     7261 agccgctcag aggaaattat atcgatgacg gaggtttgcg tacagtcatt gttgcacatt
     7321 acccccccaa tgatttcggc ctgtatgata tagccggaaa tgtctcggaa tggacaaata
     7381 cagcatttga tccgtcgact tataacttca cctgggatat gaatcccaat tatacataca
     7441 acgccaagga agatgacccg cctgccctga aaagaaaagt tgtacgcggg ggctcgtgga
     7501 aagatattgc atactttatg caggtatcca cccgtgatta tgaataccag gatactgcca
     7561 agagctacct gggattccgt tgtatacttc cttacctcgg ccgtaataaa gatgacaatc
     7621 cggccagggc atcaagggtc tataactaat tcgttcagaa attctcatta aatcaaccaa
     7681 taaaaactta tttattctaa tatcatgggt ctcaacacat ttgtcagaag gcgcggtttc
     7741 aggaatttca tgtccaaact ttacggctgg ggcgcatcag tcgttattct cggcgctttg
     7801 ttcaaaatca accattaccc gtttgcaaat gaaatgctta tcgtgggttt gggaacagaa
     7861 gccatcattt tcttcttctc agcgttcgaa cctccgtatg tcgagcccga ctggagtctc
     7921 gtttacccgg aactgggagg tttataccat ggcgatgaaa cagcggaatc ggttaaaaag
     7981 aagcccacac agcaattgga cgatctgctg aaacaatcga atatcgacca gaaactaatg
     8041 gaccgactcg gcgacggact ccggaaattg agcgacaata catccaaact ttccgaaata
     8101 accgatgcag ccggggtaac caatgagtac ctcaccaaag ttaaaggcgc tgccaaatcg
     8161 gttgaaaacc tttcaaaatc ttatgatgtc accgccgaaa cccttcagaa agatgtgaat
     8221 gcttcggctg gatattccaa cagcataaaa gctgccggag aacatgccgc tcagctttcc
     8281 aatgtatata aggaagcttc tgaaactctc aaaggcgata tcaatgtcac caaagagttt
     8341 accaacagca tgaaaactgc tatgcagtca gccaattcac tggccgaaca gtataccaaa
     8401 tcggcagaag ccctctcact ttccgctaaa caactcgact ttgctaccgt cgacggaaag
     8461 tcctatacgg aaaaacttca tcagatatcg gaaaaactgg ctgccctgaa ttcagtctat
     8521 gaacttcagc tgcagagtaa taaggatcag atgcagtcga ctgctcaggt ccgcgattcc
     8581 atggcacagc tgctcaaaac aatgaaagaa tcagccgata cgatggcaac ctataaggac
     8641 cagatgaact tattgacgca gcggatctct tcgctcaacg aagtgtacgg aggtatgctg
     8701 acagctatga gcgggaaagc caaatagtca atgcaggcaa cagttaacca gatttttaac
     8761 aagattaaat ccgaaagata tggctggata taaagagaca ccacggcaga aaatgatcgc
     8821 catgatgtat cttgtgctga ctgccctgct ggctcttaat gtttccaagg acatgctcga
     8881 ggctttcctc gtggtaaacg aaagtatgga gaatactatc gaagtctttg aaaataagct
     8941 tggaaacctt tatatggatt ttgagaagca gtattcactc aaaccgcaga aagttggcga
     9001 ttactgggaa aaagcacaga aagcacgaaa attatcgaat gaacttaaaa attatgtgga
     9061 tcatgtgatc tttgatgtgg tccgacaatc ggaagggaaa gattctgtca aattgatgca
     9121 ggacgcatat gaaatgagaa cgatgcgcga cccttacaat ccgagtaagg atatccaggt
     9181 ccccctgttg aatatgaata atgtcgctac caaggataaa tacgacgaat cgaccaatta
     9241 tttcataaac aaaggcaagg cggaggaatt aaaagaaagg atcgagaaat ataaagccga
     9301 tatgatcgct ctcgtaccgg aacagttcag gcaaaatatc caactgggcc ttgaaacgga
     9361 tggggattat tacaatgcag acggcgaaaa acaaacatgg gaattgcatc atttctacca
     9421 taccatcctg gcagcatcgg taacgatcct gaataaaatc aaagcagaag tacagaccgc
     9481 cgaattcgat atcacagccg agttgctcag cgaaattgat atttccgact ttaagttcga
     9541 taaaattgaa gcaaaggtca tccccacgac gaactatgtc ctccagggta acaagtacga
     9601 agcggaagtt ctcgttgctg cttttgatac ccggcaaacc ccggaagttt tcgtgcttca
     9661 gggtgccgag gagatcactg taagcaataa ggaccgtgca cagaaagtcg ccggtgaaca
     9721 aggtgtggtg aagctgtcat tcccctcagg aaatgtcggc ccccagaaat atgcgggtgt
     9781 cgttgaggtg gtatccccgg aaggagaacg gatcccctac aacttttccg gtgaatatgt
     9841 ggttgctcct ccttcattga cggttgcagc cactaaaatg aacgtatttt acatcggtgt
     9901 ggataacccc gtttcaatct ccgttcccgg gattgcagat gccaacctga agccttcgat
     9961 ctcaaccgga acccttacac gagaccctga gggtaaagac tggattgtca aagttccgca
    10021 aggtcaacct aaagccatta tatctgtcga agccctttac cagggagaaa cggtaccaat
    10081 gggctctaaa gagtttaggg tcaggagagt cccggatccc agggctgaaa ttgccggcca
    10141 ggtggaaggt ccgatcgata aaaacacact gttggctgcc ggggctatta tccctgttat
    10201 gaaagatttc gaattcgatc tctattttga agtcaaatct ttcaggctta ccactatcat
    10261 cgggggcgac gggatatcca agcgcgccaa taataaccgg ttatccgatg aaatgatcag
    10321 tatgatcaga tcagcccgga gcggtcagaa attcttcttt gaaaatatta cggctgaagg
    10381 cccggataaa acaccccgaa gcctgaaccc tataactttg gaaattaaat aataccaccg
    10441 gaggcactat gaaaaatatc gcattgcttt tcatctcatt gatcatcctg gcaggaatct
    10501 ctgtgcctgc aaaggcccag gtgctcagcg acccgccgaa agatgtcatc aatttcgaca
    10561 atgagctcct ggatgtcaaa ccagtacctc tgcccgccat ccgtcaggcc gacatgatgt
    10621 ggtcaaaacg aatttggcgg gaaatcgata tgcggcagaa gatcaatcag cccttctatt
    10681 acccgatcga accacacgat aactggcgca acttcatcag tgtcattatg gatgcactca
    10741 aagaggggac gattacagcc tatgatattt ccaatacgga tgaatttact atcccgctga
    10801 attaccagga aatcatttcc aggcagatcg ataccatgca caaaaccttc cagcggcctt
    10861 atccacctta tgaagaatac gatacggtta tctatagtga gtttaatccc agtaaagtta
    10921 cccgaatcag aattaaggaa gactggtatt ttgataagaa acattcccag atgctggtca
    10981 ggatactcgg tatttgcccg gttatgacca aagaaaggaa taacgaggaa tacaatgaag
    11041 cccttttctg gatttatttc ccggaagcaa gaccggtact ggctcatgcc caggtcttca
    11101 accgttttaa cgatgcctcg cgcagaacct acgacgaaat atttattaaa cggctgttta
    11161 acagttatat ttataaagaa caaaatgtgt acgaccgcag aatccaggag tatgcgcagg
    11221 gcctcgatgc cctgctcgaa gcggaaagga ttaaaatgga actcctgaac ttcgagcaaa
    11281 gtctctggga atactaaaaa cttagccata tttaacaccc gaaagccgcc caaccatctg
    11341 aggcggcttt tttttatgtg cgcccggcat gggcgataac tatagggtga aagacccgaa
    11401 cacacttgg
//
LOCUS       DAOPNH010000078        11371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6053_length_11371_cov_6.904295, whole genome shotgun sequence.
ACCESSION   DAOPNH010000078 DAOPNH010000000
VERSION     DAOPNH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_11371_cov_6.904295"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..3459
                     /locus_tag="PKZ74_05665"
     CDS             136..3459
                     /locus_tag="PKZ74_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPM92279.1"
                     /translation="MEPGFAEVLYTQILESHVRNEDHHINAIRDFRLTFEQVFRKLTE
                     GEAQVFSNTFQRVVFVIDKFQVPREVSDEMHGFRMLANKITHDEGATVAEKTYYTALR
                     AVCLTVNHFSNMPVPGTLLAIYDHMAGLSFSSRSFQSYEEVEWAKCVVKGKSNLETRP
                     DQKVQFILNCEAEEDPGLFKLVVRADDKNFFRELHPLISDYSTLNLIRIRKDQWQNDT
                     YLTGPETRIVLEPDFLIDASDIAECFQRSGANPMLYFLNKLVDAEGGEGAFKGKIVND
                     HLDSLIKTPAKTPERVFQEAVESNALQSARFGRDAMERIRGDIKTVHFGNLRTTAEQL
                     AGKKVRIEPTFFSALYGLQGRLDVLTEDPGNPSRKDIFELKSGARVPSGDVWRNHKMQ
                     VVCYNMLLSSTFGKQRVGTSSILYSAAASNPVRNVASVIFDEWQVLHVRNHIAGGLFR
                     LSNGDYSLLRRINSHDFGEAPPYKTDDIGAFEGAYHLASEMEKKYYQHLLTFCLKEHQ
                     VAKIGSDRDSGREDSGFSALWQETLPEKLKKYNILCGLVYQRFDSAANTVELKMDDPG
                     LNHNFREGDLGIIYPKDPDMPDPLKSQILKGNIESLDNGTLIFRLRNKQLDETYFGSQ
                     REWIIEHDLYESNTWSVVQSLLRFLQAPARLKELIFGLSEPQFDDLPAVTVKELTPNQ
                     NSLLNSALRARDYFLMQGPPGTGKTSTMMVEVTRRLIASTKSRIVILAFTNRAVEEIC
                     ARLRGVGIAFLRLGGRNADDEDALHSLVSGKDAGQIRELIIRHRVFVSTVSSFHSRKS
                     DLFDILPPDVLMIDEASQLTEPMVVGLLPGFRKFILIGDQKQLPAVIAQSESRCRIND
                     PELNRAGIVDLRQSLFERLFRLCTKNGWTAATGMLESHYRMHDQIAALINSYYDGKLV
                     SVKPGQQKIAFDTLYDHTSGDPVERLLSRSRTLFIQSGYSPTSKRHEEEARKVVAILK
                     TIRRRFGANFTANTVGVVTPWRAQIAQIRNLIDDPEILGKVTIDTIERYQGSERDIII
                     VSLAVYHQNQLIPLQSVDLDDTVDRKLLVTVSRAKNQLIMLGFDRPLEGSRYYRDLME
                     KIGQNGGMIGYLDSMNVFR"
     gene            complement(3472..4443)
                     /locus_tag="PKZ74_05670"
     CDS             complement(3472..4443)
                     /locus_tag="PKZ74_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92280.1"
                     /translation="MYKELLSSLIFGQLQSYGSIAAFPLSIPLKPGPFDYLTLSEALS
                     MLLIRIEEVSESGSVPELKVISQADAPVLILAGEELKGARQNRILNTSILIPVKSELI
                     IPVSCTESGRWSYNSPYFRESGNISSRDIRLSASESVSFSLDSGRGHRSDQGKVWDKI
                     EELHSRSRSDSKSHTRAMDDAYQARKADLDEARRHFNLIPGQAGILFFHSGKVAGLDI
                     LSKPGAYARLHDKLVGSYTIDVLDGSSPSWNKTALHHTARKFLDNASEAVLTPYKSPG
                     MGFDHRVQGKHLQGSMLVYNEESIHTCLFSHEPEDPPVVRYYRRREL"
     gene            4647..5840
                     /locus_tag="PKZ74_05675"
     CDS             4647..5840
                     /locus_tag="PKZ74_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92281.1"
                     /translation="MERLIEKSNKAILSTDYPYRRHVIGKINWNWKMNGIIGARGTGK
                     TTLLLQKLKELKQEGHDVLYARLDDFYFSDHRLNDLADEFRKQGGGFLYLDEIHKYPG
                     WPEELKNIYDTMPELKVVFSGSSIIDLMATDADLSRRALNYEMTGLSFREFLLMSGIS
                     NSTPFSLDEILQNHVEIAAGMIRQFPVLKYFRTYLRSGYYPYFLEPDRDYLLTLEQVI
                     RTVMEVDMRFIENYDVTQSRKMLLLLKIIASSAPFKPNVLKISERTGLHRHTVLQYFH
                     YLEKARMIKLLNHPEKHISRLQKPDKIFLENPNLFYALNPEMVDIGNLRETFVLNQLS
                     VNHEVNLHARVDFMVDFKYLLEIGGKNKKSSQIKDLDHAFLFMDDIEIGNKRTIPIWL
                     LGFLY"
     gene            complement(5987..6274)
                     /locus_tag="PKZ74_05680"
     CDS             complement(5987..6274)
                     /locus_tag="PKZ74_05680"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM92282.1"
                     /translation="MISKITSLLAKNQAVNRLVLFGSRAKGNFKPGSDIDIAVFGHNL
                     TLDDFLVFSINIDQLELANKVDLVHYEKIKEKELIEHIARVGIVLYEKAEE"
     gene            complement(6333..6746)
                     /locus_tag="PKZ74_05685"
     CDS             complement(6333..6746)
                     /locus_tag="PKZ74_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPM92283.1"
                     /translation="MGDKDIRWLQRFSNLKKAFMKLKEAVEQEPLSELENEGLIQRFE
                     YTYELAWKTLEDLLEEKGYLDIKGPAPVIRQAFQDGYIADGDGWMRLKKSRENTSHTY
                     NKEIAKEIVEQIISFYFFLFEDLILKLEKEEQSKV"
     gene            complement(6926..7114)
                     /locus_tag="PKZ74_05690"
     CDS             complement(6926..7114)
                     /locus_tag="PKZ74_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92284.1"
                     /translation="MINISLPGLYGVKEVSLADINGRIVEKMSFSHQGKAADMIQIST
                     ASLPAGNISSGSPPTIRH"
     gene            complement(7111..7329)
                     /locus_tag="PKZ74_05695"
     CDS             complement(7111..7329)
                     /locus_tag="PKZ74_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92285.1"
                     /translation="MKSFFIFWMTAILIVSSLSVNSQWSCDPANPVVVCNQAGLQNNV
                     MTIADNNGGVYVYWLDARLNPGSPAREI"
     gene            8080..10506
                     /locus_tag="PKZ74_05700"
     CDS             8080..10506
                     /locus_tag="PKZ74_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HPM92286.1"
                     /translation="MKKNRIYTGIIAMMLIALASQVFSQARVQVIHNSADAAAEVVDV
                     WLNDQLLIDNFKFRTATPFIDAPAGEKFDVTIQPANSTDTTNGLARFTYQLENNKRYI
                     LVANGIVIPTGYNPATPFNIYVWDLGREAAKLPSNTDVLVFHGSTDAPIVDVVEVAVP
                     AGTVVNDLEYAEFAGYLELPTADYSLQIRDITGTTTVAEFAAPLAALGLQGSALAVVA
                     SGFLNPGNNNDGPAFGLWVALPTGGNLIPLPNVPISTARVQLIHNSADIAAQTVDIWL
                     ENELMIDNFSFRTATPFVDVPANKDLNVTIAPANSNGFSDGLASFTLNLEGGNKYLLV
                     ANGIVSANGYSPATPFSIFSYETAREMAMMEGNTDVLVFHGSTDAPTVDIVETNFGIG
                     TIVDNLSYGEFAGYLEVPSSDYTFQLRDANGKVTVATFEAPLFSYGLQNEAVTVLASG
                     FLNPQENNDGAAFGLWVALPQGGDLVPLPQVIPVTQTARVQVIHNSADAAAEFVDVWL
                     NDQLLIDNFKFRTASPFIDAPAGTDFDVTIAPSNSVSVSEGLATFTYNLAEGNTYILV
                     ANGIVVPQGYNPPTPFDIYVYDMGREMASNPANTDVLVFHGSTDAPVVDVVEVGVGAG
                     TIIDNLAYADFAGYLELPTADYQIDIRDESGTVTVVSYSAPLASFGLQGRSIAVVASG
                     FLNPGANNDGAAFGLYVALPSGGELIALDPAIPTDVTENRTDEIKASIFPNPARDNMN
                     VQFELPQASDVQVRIYDLTGTMVMSRQFSASQAASQQRIDIGNLPGGLYFVKTQAGNS
                     SFVTRLTKVN"
     gene            complement(10579..>11371)
                     /locus_tag="PKZ74_05705"
     CDS             complement(10579..>11371)
                     /locus_tag="PKZ74_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92287.1"
                     /translation="SYNAAQYKIDPGHILLGGSSAGAITALNTAFMDEDERPESTGGN
                     IWQLQKDLGGLDESGNDISGQYTIRALLNLWGAVNDTAIIDAGEKIPVLSVHGDADKI
                     VPYGYNYPFLDMDTNVTSNIVSKLYGSQYIHRRLVHLGFESELITLRHAGHEPQYEPG
                     KYRAVMDTIISRSTDFYFRSLLHFPDIAGPRQVAIGMSPSSYSVPVQEGVSYYWQATG
                     GKVLTGSFKNEAKVVWLAKEKGMVSVVLYHRNGANAEVRLPVVLP"
BASE COUNT         2821 a         2829 c         2949 g         2772 t
ORIGIN      
        1 aaaatcgcaa ataccgaaaa atcggtacag gctctgttcc gactcggtat aaccacatca
       61 ggcacatagg acacaatagg gaacacaata gattctttgt taattttacg gaaaacaacc
      121 caactcaact gtgccatgga accgggattt gctgaagtgc tttatacaca gatacttgaa
      181 tcgcatgtac gaaacgaaga ccatcatata aacgccatcc gggatttccg gctgacgttc
      241 gagcaggtct tccgcaagtt gacggaaggc gaggcacagg tgttttccaa cacgttccag
      301 cgggtcgttt ttgttatcga taaattccag gtgccacgcg aggtgagcga cgagatgcac
      361 ggtttccgca tgctggccaa caagatcact cacgacgagg gggcgacggt cgccgaaaaa
      421 acctactaca cggccttgcg ggcagtctgc cttacggtga accacttctc caatatgccg
      481 gtgccgggga cattgctggc aatctatgat cacatggccg gtttgtcgtt ctcctccagg
      541 agcttccaat cctatgaaga ggtcgaatgg gccaaatgcg tggtcaaagg gaaaagcaac
      601 ctggagacac ggcccgacca gaaggtgcag ttcatcctca attgcgaggc ggaagaggac
      661 ccgggacttt tcaagctggt ggtgcgggcc gacgataaga actttttcag ggaactgcat
      721 ccgcttatct ccgattattc cacacttaac ctgatacgca tccggaaaga ccagtggcag
      781 aacgatacct acctgacggg cccggagacg cgcatcgtcc tcgaacccga cttcctgatc
      841 gacgcttcgg atatcgccga gtgcttccag cgttccggcg ccaacccgat gctctatttc
      901 ctgaataaac tggtggacgc ggaagggggc gaaggggcgt tcaaggggaa gatcgtaaac
      961 gaccacctcg attcgctgat caaaacaccc gcaaaaacac cggaacgggt gttccaggag
     1021 gcggtggagt ccaatgccct tcaatcggcc cggtttggaa gggatgcgat ggaaaggatc
     1081 aggggcgata tcaaaacggt ccatttcgga aacctgagaa ctacggccga acagcttgcg
     1141 ggtaaaaaag tcaggataga gcccacattc ttctctgccc tttacgggct gcagggacgg
     1201 ctggatgtgc tcacggaaga tcccggcaat ccttccagga aagatatctt tgaactgaaa
     1261 agcggcgcac gcgtgccttc cggcgatgtc tggcggaacc acaagatgca ggtggtttgt
     1321 tacaacatgc ttctgtcgtc caccttcgga aaacagcgcg tggggacctc ttccattctc
     1381 tactccgccg cggcatcgaa tcccgtgagg aatgtcgctt cggtgatctt cgacgaatgg
     1441 caggtgctgc atgtccgcaa ccatattgcc ggaggcctgt tccgtttgtc gaacggggat
     1501 tacagtctgc tccggagaat caacagccat gatttcggtg aggcgccgcc gtataaaacc
     1561 gacgatatcg gggcgtttga aggggcgtac cacctggcca gcgaaatgga gaaaaaatac
     1621 taccagcatc tcctcacttt ctgcctgaag gagcaccagg tggccaagat cgggtcggac
     1681 cgtgacagcg gacgcgaaga cagcggattt tcggccctgt ggcaggagac gctgccggaa
     1741 aaactgaaaa aatacaacat cctttgcggg ttggtttacc agcgcttcga cagcgcagcg
     1801 aacaccgttg aactgaagat ggacgatccc gggctgaacc acaatttccg cgagggcgac
     1861 ctggggatca tctatccgaa ggatccggac atgcctgatc cgctgaaaag ccagatcctg
     1921 aaaggaaata ttgagagcct tgataacggc acgttgatct tcaggctccg caacaagcag
     1981 ctcgacgaga cctatttcgg cagccagcgg gagtggatca tcgagcatga cctgtacgaa
     2041 tcgaacacct ggagcgtggt gcagagcctg ctccggttcc tgcaggctcc cgcccggttg
     2101 aaagaactga tcttcgggct gagcgaaccc caattcgacg atttgccggc ggtgacggtt
     2161 aaagagctga cccccaacca gaacagcctc ctgaacagtg cactccgggc ccgggattat
     2221 ttcctgatgc agggccctcc cggtactggc aaaacgagta cgatgatggt ggaggtaacg
     2281 cgaaggctta tcgcatccac caaaagccgc atcgtgatcc tggcctttac caacagggca
     2341 gttgaggaga tatgcgcacg gctgagaggc gtgggcattg cgttcctccg cctcggaggg
     2401 cgcaacgccg atgacgaaga cgcgctgcac agcctggtgt cggggaaaga tgcgggccag
     2461 atcagggagc tgatcatccg gcaccgggtc tttgtttcga ccgtatcctc attccattcg
     2521 cggaaatcgg atttgttcga catcctcccg ccggatgtgc tgatgatcga cgaggcttcg
     2581 caactgaccg agccgatggt ggtgggactg ctgccgggat tccgcaagtt catcctgatc
     2641 ggcgaccaga aacagcttcc ggcggtaatc gcccagtcgg agagccggtg ccggatcaac
     2701 gacccggagc ttaacagggc ggggatcgtc gacctgcggc agtcgctgtt tgagcggcta
     2761 ttccggcttt gcacgaagaa cgggtggact gcagctacgg ggatgctgga gagccattac
     2821 cggatgcacg atcaaattgc ggctcttatc aacagttatt acgacggcaa gctggtttcg
     2881 gtaaaaccgg gtcagcaaaa gatcgcattc gacaccctct acgatcatac ttcgggtgat
     2941 cctgtcgagc ggctgctgtc gcgttccagg acgctgttca tccagtcggg ctattctccg
     3001 acgagcaagc ggcacgaaga ggaggcaaga aaggtggtgg cgatactgaa gaccatccgg
     3061 cggagatttg gcgcgaattt cacggcgaac acggtgggtg tggtgacgcc gtggcgggcg
     3121 cagatcgccc agatcaggaa cctgatcgat gacccggaga tcctcgggaa agtgaccatc
     3181 gacacgatcg agcgttacca gggctcggag cgcgacatca tcatcgtttc gctggccgtg
     3241 tatcatcaga accagcttat cccgctccaa tcggtggatc ttgatgatac cgtcgaccgg
     3301 aaactgctgg ttacggtttc ccgggccaaa aaccagctta tcatgctcgg tttcgaccgg
     3361 ccgctggagg ggagccggta ttatcgcgac ctgatggaga agatagggca gaacggcgga
     3421 atgatcggtt acctcgacag catgaacgtg tttcgctagt tccaggctga tttacaactc
     3481 tctcctcctg tagtacctga ccacaggcgg atcttccggt tcatggctga agaggcaggt
     3541 atggattgat tcttcgttat aaaccagcat gcttccctgg aggtgtttcc cctggacacg
     3601 gtggtcgaat cccataccgg gcgatttata gggtgtcagc acagcctccg acgcgttatc
     3661 caggaatttt cgcgctgtgt ggtgcagggc ggttttgttc caggaggggg aggagccatc
     3721 cagtacatcg atggtgtaac tgccgaccag cttgtcgtgc agacgggcgt aggcgccggg
     3781 ctttgagagg atgtcgagcc cggcaacctt accggagtgg aaaaagagaa tgcccgcctg
     3841 acccgggatg aggttgaaat gccgccgggc ctcatccagg tctgcttttc tggcctggta
     3901 tgcatcgtcc atggcgcgcg tgtgcgactt cgagtcgctc ctgctcctgg aatgaagttc
     3961 ttctatttta tcccacactt tcccctggtc ggagcggtgt ccgcggccgc tgtcgagact
     4021 gaagctgacc gattcgctgg cggagagacg gatgtcgcgg ctgctgatgt tacccgattc
     4081 acggaagtag ggagagttat agctccacct tccgctttcg gtgcagctta ccggaatgat
     4141 gagctcgctt ttgacgggga tgagaatgga ggtgttgagg atcctgttct gccgcgcccc
     4201 tttcaattcc tcgccggcga ggatcaatac gggcgcgtcg gcctggctga tcactttcag
     4261 ttcggggacg gagccgctct cgctgacctc ttcgatccgg atcagcagca tggataacgc
     4321 ttcggacaag gtgagataat cgaacggtcc gggtttcagg gggatcgata gcgggaatgc
     4381 agcgatggaa ccataggatt gaagctggcc gaagatgagt gatgataata attctttgta
     4441 catggtcggt taatttgccg acaaaggaaa aaaggggagg tgcagtgtat ttgcatttgc
     4501 tgaatggatt ttttttggtg gagaaagaag gcttagtccc gtagggacgc cagtatggta
     4561 gaccgggaac aggaattaaa tatttcaata ttggttatta taatcaccaa aatagaatta
     4621 tatttgttga atcgaatcat cagaatatgg aacggcttat tgaaaaatca aataaagcga
     4681 tcctttccac cgattatcct tatcgtcgtc atgtgattgg taagatcaac tggaactgga
     4741 aaatgaatgg aatcatcggt gcaaggggga cgggaaagac gacgctattg ctccagaaac
     4801 tgaaagagct caagcaagaa ggtcatgatg ttctctacgc cagattggat gatttttatt
     4861 tctccgatca ccggctgaat gatctggctg atgagttcag gaagcaaggc ggcgggtttc
     4921 tttacctgga tgaaattcat aagtatcccg ggtggccgga agaactgaaa aatatttacg
     4981 ataccatgcc agaacttaag gtcgtatttt ccggttcttc cattatcgat ctgatggcaa
     5041 cagatgccga cctgagccgg agagcgctga attacgaaat gaccggtctg tctttccgtg
     5101 aatttctcct catgtcaggt atctcgaatt ccactccttt ttccctggat gaaatactgc
     5161 agaaccatgt cgaaatcgct gccggaatga tcaggcagtt tcctgttctg aaatatttcc
     5221 gtacatacct gagaagcgga tactatccgt attttctcga accggaccgc gattacctgc
     5281 ttaccctcga acaggtgatc cggacggtca tggaagttga tatgcggttt atagagaatt
     5341 acgatgttac ccagagccgg aaaatgcttt tgctattgaa gataatcgct tcttctgcgc
     5401 ctttcaaacc caatgtattg aaaataagtg aaaggaccgg cctgcaccga catactgtgc
     5461 tgcaatattt ccattacctc gaaaaagcca ggatgatcaa attgctgaat catccggaaa
     5521 agcatataag ccggctgcag aaaccggata aaatctttct cgaaaacccc aacctgttct
     5581 atgccctgaa tccggaaatg gtcgatattg gaaatctcag ggagacgttt gtcctgaacc
     5641 agttatctgt aaatcatgaa gtgaatctgc acgcccgggt tgattttatg gtggatttta
     5701 aatatctgtt agagatcgga gggaaaaata agaaaagtag ccagatcaag gatcttgatc
     5761 atgctttcct gttcatggat gatatcgaaa tcggcaacaa aaggaccatt ccaatttggt
     5821 tactgggatt cttgtattga tataccaatg tccctgggaa aagacaggag acagaattca
     5881 ccgcagatta cacagattta caccgagtta acacagagac ccatcactca cccccccccc
     5941 tttgtcctgt agggacgata gtatggtagc taaaatcaca ggtcattcat tcttcagctt
     6001 tttcataaag cacaatcccg acacgcgcga tatgttcgat aagttccttt tccttgattt
     6061 tctcgtaatg aacaaggtct accttattgg ccagttcaag ttgatcgata tttattgaaa
     6121 aaaccaggaa atcgtcgagt gttaaattat gaccaaaaac cgctatgtca atatctgaac
     6181 cgggcttgaa atttcctttc gccctggagc cgaataatac gagcctgttt acagcctgat
     6241 tttttgccaa cagggaagtg attttggaaa tcatatcatt tttaagcccg tatggcataa
     6301 agcagacttt tagtattaat tgcgatcaat gtctatacct tactttgttc ttccttttcc
     6361 agtttgagga tcagatcttc gaacagaaag aaatagaatg aaataatttg ctcaacaatc
     6421 tctttggcga tttctttatt ataggtatgc gatgtgtttt cacggctttt ctttaaccgc
     6481 atccagccat caccatcagc aatataacca tcctggaacg cctgcctgat caccggtgcg
     6541 ggtcctttaa tatccagata tcctttttct tccaaaagat cctccaacgt cttccaggca
     6601 agctcgtagg tatattcaaa ccgttgtatt agcccttcgt tctcaagttc tgataacggt
     6661 tcctgctcaa ccgcttcttt aagcttcata aaggctttct tcagattgct gaaccgctgc
     6721 agccaccgta tgtctttatc acccataatc aataattatt gaggatcaac aaatatagtg
     6781 aatgtcgggg agaatagggc gggtaaaaga tgtgaaatgc tttaaggctg gaaaatcatt
     6841 caccacagat ctacacaact tgtcccgcaa ggcgggagag ttaacacaga gtgaactctt
     6901 ccttacctga cgatgaccag gtgtttcagt gtctgatcgt gggtggcgac ccggaggaaa
     6961 tattgccagc ggggagcgat gcggttgata tctgtatcat atccgccgct ttgccctggt
     7021 ggctgaacga catcttctcc actatccttc catttatatc tgccagtgaa acctctttca
     7081 cgccatacag gcccggcagg gaaatgttga tcatatctca cgggcaggtg agccgggatt
     7141 cagccgggca tcgagccagt aaacataaac accgccgttg ttgtcggcta tggtcatgac
     7201 attgttttgc agaccggcct ggttgcacac aactaccggg tttgccggat cacacgacca
     7261 ttgtgaattg actgaaaggg atgatacaat tagaatagca gtcatccaaa atataaagaa
     7321 tgatttcatg gtattgcgat ttagtgtaag caggaaaaat tggattgcgg caaaattaca
     7381 tgcagggaaa ggataccggt ggattttaat attagattaa caaatttggg ccatgaatct
     7441 ttattcagca gagagtttac gtaggaatag gaggttgagt aaaatattag atgcttaatg
     7501 ttgagtccca tccgaaaagt gtatttaccc acccctaacc cgaggctgtc tcaaaagcct
     7561 tatttatcga attgctctca attatttgtt cgtgctgagg ctgtatcaaa agtaataaac
     7621 cacaaaggga caccaaggat ttcacgaagg aacacaaagt ttattatttg ataaccagat
     7681 acttagtgcg actttgtgat gtactttgtg cgactttgtg gttgaatatt agacttttga
     7741 gacatccccg gaaatatcca attaatccag aatgctgata ttaaattaat tacatagatt
     7801 gaagctatac ccctcccttt ataagggagg agcaggggtg ggtacttttg gatagagatt
     7861 tctattcgga gtagactcaa tgcttattga gttatgttta atgattatga caaaatatat
     7921 taaaggtatt atgtttttaa aattcataaa aagttataaa acaatgatat aagaaaatat
     7981 attgaatata atgtttaaga aaaacattaa ataaatgaac aaaaatacta aacctgctgt
     8041 ttagctttca tgaaatttaa ccctataaaa ttaaacatta tgaaaaagaa cagaatttac
     8101 accggaatta tagccatgat gcttattgca ctggcatcac aggtcttctc tcaggccagg
     8161 gtacaggtta tccataattc agccgatgcc gccgcggagg tggtcgatgt ctggctgaac
     8221 gaccagttac tgatcgacaa tttcaagttc cgcacggcaa ccccgtttat cgacgccccg
     8281 gcgggagaga aattcgatgt caccatccag ccggccaact ctaccgatac caccaacgga
     8341 ctggcccgtt ttacttacca gcttgagaac aacaagcgtt atatcctcgt cgctaacggc
     8401 attgtgatcc ctaccggtta caatccggcc acaccattca atatttatgt ctgggacctg
     8461 ggacgcgagg cggctaaact tccctccaat accgatgtcc tggtcttcca tggctcgacc
     8521 gatgcaccga ttgtggatgt ggtagaagtg gccgttcccg caggtaccgt ggtgaacgac
     8581 ctggaatatg ccgaatttgc cggttacctg gaattgccga cagccgacta ttccctgcag
     8641 atcagggata ttacgggcac cactaccgtt gctgaattcg ccgctccgct ggctgccctc
     8701 ggattacagg gcagtgcact ggccgtcgtg gcatcgggct tcctgaaccc gggcaataac
     8761 aatgatggcc ccgctttcgg gctctgggtc gctttgccga ccggcggcaa cctgatcccg
     8821 cttccaaacg ttccgatctc cactgcaagg gtgcagttga tccacaactc cgccgatatc
     8881 gcggctcaaa cggtcgatat atggcttgaa aacgaactga tgatcgacaa tttcagtttc
     8941 cgcaccgcca ctccgttcgt ggatgtgccg gccaataaag atctgaatgt gaccatagca
     9001 ccggctaact ccaatggttt ctccgatggc ctggccagct ttactttaaa cctggaaggc
     9061 gggaacaaat atttgctcgt cgccaacggt atcgtttcag ccaatggtta cagccctgca
     9121 acgccattca gcattttctc ctatgaaacg gcccgtgaaa tggctatgat ggaaggcaac
     9181 accgatgttc tcgtgttcca cggatccact gatgccccga cggtcgacat cgtcgagacc
     9241 aacttcggaa tcggcaccat cgtcgacaac ctttcttacg gtgaatttgc cggctatctc
     9301 gaagtgcctt cttccgacta tactttccag cttcgtgatg cgaacggaaa agtgaccgtt
     9361 gccactttcg aagctccgct attctcatac ggtcttcaga atgaagctgt aaccgttctt
     9421 gcttccggat tcttaaatcc gcaggaaaac aacgatggag cggcattcgg actatgggtt
     9481 gcgctgccgc agggcggtga ccttgtaccg ctcccgcagg taattcctgt cacacagacc
     9541 gcgcgggtcc aggtgatcca taactcggcc gatgccgctg ctgaattcgt tgatgtatgg
     9601 ctgaacgacc agttgctgat cgacaacttc aagttccgca ccgccagtcc gttcatcgac
     9661 gccccggccg gaaccgattt tgatgtcacg attgcccctt ccaactcggt gagcgtttcc
     9721 gaagggctgg ccacattcac ctacaacctg gctgaaggca atacttatat tctggtcgct
     9781 aacggaattg tggtccccca gggatataat cctcctacgc cattcgatat ttatgtttac
     9841 gatatgggcc gcgaaatggc ctccaatccg gccaataccg atgtgctggt attccacggc
     9901 tcaaccgatg caccggttgt agatgtggtg gaggttggcg ttggcgccgg cactatcatt
     9961 gataacctgg cctatgccga tttcgccggt tacctcgaac tacctacggc cgactaccag
    10021 attgacatca gggacgaaag cggcacagtg acggtcgttt cctacagcgc tccgctcgct
    10081 tcattcggac tccaggggcg gtcgatcgca gtcgttgctt ccggtttcct caatcctggc
    10141 gcgaacaacg acggagccgc tttcggtctc tatgttgcgt tgccttcagg cggtgagctg
    10201 atagcccttg accctgcgat tccgaccgat gttacggaga acaggacaga tgagattaaa
    10261 gcttccatct tcccgaatcc ggcacgtgat aatatgaatg ttcagtttga gctgccgcag
    10321 gcttccgatg tgcaggttcg gatctacgac ctgaccggta ccatggtgat gagccgccag
    10381 ttcagcgctt cgcaggcagc cagccagcag cggatcgaca ttggcaacct gcccggcgga
    10441 ttgtactttg tcaagaccca ggccggtaat tcaagctttg tgaccaggct gacgaaggta
    10501 aattaagata gtggtttgtt gagaaccata tatttagtta ataatgcgaa ggctgcctca
    10561 ttcaagggca gccttttttc atggtaagac caccggcagc cggacctctg cgttggcgcc
    10621 gttgcggtga tataaaacga ccgataccat tcccttctct ttagccagcc agaccacttt
    10681 tgcctcgttt ttaaagctac ctgtcaagac ctttccaccg gtggcctgcc agtaatacga
    10741 cacgccctcc tgcaccggaa cggaatagga tgatggcgac atgccgatgg ctacctgccg
    10801 cgggccggcg atatcgggga aatggagaag tgaacggaag taaaagtcgg tggagcggga
    10861 aatgatcgta tccatgaccg cccggtactt tccaggctcg tattgcggtt cgtggccggc
    10921 atggcggagg gttatgagtt cgctctcaaa tcccagatga acaagtcgcc ggtgaatgta
    10981 ttgcgaaccg tacagcttgc tcacaatgtt actggtcaca ttggtgtcca tatcgaggaa
    11041 cgggtaattg taaccgtacg ggacgatctt atcggcgtcg ccgtggacac tcaggacggg
    11101 aatcttctcg ccggcgtcga tgatggcggt atcgttcacg gctccccaga ggttcagcag
    11161 ggccctgatg gtgtattggc cggagatgtc gttcccgctt tcatccagtc cacccaggtc
    11221 tttctgcagt tgccagatgt tgccgcctgt gctttcgggc cgttcatctt catccataaa
    11281 agccgtattg agggcggtga tggcgccggc gctggatccc cccagcagga tatgaccggg
    11341 atcgattttg tattgtgctg cattgtacga g
//
LOCUS       DAOPNH010000079        11275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6131_length_11275_cov_5.473708, whole genome shotgun sequence.
ACCESSION   DAOPNH010000079 DAOPNH010000000
VERSION     DAOPNH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_11275_cov_5.473708"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PKZ74_05710"
     CDS             <1..1264
                     /locus_tag="PKZ74_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92288.1"
                     /translation="VSDFSFLGTEGYDGVYLSAFDLAANQTFAYQYDNTIPDWVNLSD
                     ETTPIPMSAGLAISTTTNNPQLLSMTGKMIHGAYDTDNGGSGPSVVNLGWNLIANPYP
                     SGLGVYEVLDENAGVIDEDIYVWEGLNNAEGGNYSAYNYTAGEGTGGLFDGLMGIGQG
                     FFINALSTPVSFTNNNVRFHTSEVLLKNNQLYKLRLSASGNGFKDEFVVHFSEAGSDS
                     YGPGDSDKWPSMYGNATEAWTVSPDNVNLTIQTLSPLGENMVSVPTSFKCGASGTYTI
                     NASNFESFDVGTEIYLEDLQVGGDWHNLGVNPVYTFSATPDDAQARFVLHFFGPTSVD
                     DPDDEMADVKDVRIYGYGHDAYIVNFGSETVKEFVAYDLMGRELHRGTLPNNSVNKVQ
                     IGEVSAYYIVKVITKEGNVYTGKVYITK"
     gene            1340..1540
                     /locus_tag="PKZ74_05715"
     CDS             1340..1540
                     /locus_tag="PKZ74_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92289.1"
                     /translation="MGLLIIVPVIIVSASAPPPPPPGPGGTDDPIGGGAAPIGSGIVM
                     LISMGAAYGAKKVYDARKRLEE"
     gene            complement(1603..3555)
                     /locus_tag="PKZ74_05720"
     CDS             complement(1603..3555)
                     /locus_tag="PKZ74_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92290.1"
                     /translation="MLKHLRQGDRKDSPCLPFSLPPRLPFSHSPHLLITLIALFPLLL
                     FSQISQKGTPESFRYNLPAAREHILTMPERSILDAEDQESEKNGLNRRIGIAVFAGLD
                     VMNDGDVFLTDEGTQIWRLEVTCSGALAIGLHFDRFFLPEGFSLYAYSPGKNSVLGAY
                     TLYNNKPQGIFSIELTTGDRVIIELNAIAGADISGARCHLGEISCVYRDLPDRIAGRS
                     SSDDCEVNINCPEGDNWQFQKQGVVRIYVRDGGGFFWCTGSLVNNTRQDNTPYLLTAD
                     HCAPYVTPEELAQWIFYFNYEYPGCENISQTPELLTMNGAAKLANYDTSGSDFLLLRL
                     DNEVPPHYDPFFNGWNIENSASFSGVTIHHPAGDVKKISTYTDQLTSSQWSGEFGTHW
                     QVYWSATESGWGVTEGGSSGSPLFDNYGRIIGTLTGGQAACEPGGGGPNTGPDQPDFY
                     GKFSYSWDQNGTDSSQQLKYWLDPDNTGISFLPGKFTQLTAAFNADPVITLIGNDVIF
                     TDYSSGDPMAWEWYFEGGDPETYNGDGPVTVNYPNGGKFDVRLTVTDGVAYDTLYLKD
                     YIHIVGKVYPNPTGGLVSIYITEELPSDIKAEVFTIPGLKVFEQTFTEQTYPLITLDL
                     SGYSSGLYLIRLTIKQRYLFVKVIVR"
     gene            complement(3583..6075)
                     /locus_tag="PKZ74_05725"
     CDS             complement(3583..6075)
                     /locus_tag="PKZ74_05725"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM92291.1"
                     /translation="MKMNCLRFGLLLLPTLFLTAFTSFAQVSEGGLPPSFSLQGLSSQ
                     VDRLVYPAPDMEILMAEDEQNLRSAFPGPERMAYSVPVNLDVRNAGTVEILADGSILR
                     RMAVYIPGALALGVYYSDFHLPDGAKLFLYNEAQTQVIGAFTDANNHESRLFANEFIQ
                     GDLVNIEYLEPAGTSGDVSVIISEIAYAYRYIDFVENGNRDQSWSCMINVKCPEGNGW
                     ENQIQGVARLSIKIGWSYYWCSGSLINNTNNDRTPYLLTAEHCGDGANASDRNQWVFY
                     FNYQSATCTGNYGPSSNTMTGCTLKANDPNDNEYLGADFELVKLNGTPPSGYNVYYNG
                     WNRTNNAADSGVCLHHPAGDIKKVSTYQNMVSSTWWNGTPSHWKVTWIETESGLSIMQ
                     GGSSGSSVFDENGLIMGDLSGGYSSNSCTNPSPAWFGKIWYAWDQNGDVPAERLKDWL
                     DPTNTGVYTHPGLSSQILPPVVDFEADTTYLLQGESVHFTDLTTGNPALSWEWSFPGG
                     TPNASDIQNPTITYNQAGTFDVTLTVTNADGEETELKPGYITVEQVLLPDADFVASAL
                     EVTEGDMIDFTDLSTNNPTAWDWIFNGGDPATSGEQNPDSVIYAVPGVYNVYLTASNN
                     GGSNTELKENYITVLAGAPPVSDFYADKTEILPGDTVNFFDLSTGNPTQWTWTFEGAA
                     TGTSSVQNPENIVYPTEGTFYVRLRTKNFFGNNTIQKDGYITVGNISVGEINRQQGVM
                     AFPNPTAGLLTVKLPEGAEAWTGRPVQGGNIQSSVNIEIVNSLGNVIRKAEFHADYQG
                     VTFDLTDEPSGLYFVRVSTPTRSVQKKISLLR"
     gene            6239..7210
                     /gene="rfaD"
                     /locus_tag="PKZ74_05730"
     CDS             6239..7210
                     /gene="rfaD"
                     /locus_tag="PKZ74_05730"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPM92292.1"
                     /translation="MGKIVITGAAGFIGSCMAGKLNHKGYREIVLVDNFTNPRKQKNY
                     CHKSIAEKVERDDFFAWLKQHADEVEFVFHLGARTDTTETNPEVFDRLNLGFTRDVWK
                     LCTEQRISLIYASSAATYGGGEHGYSDDHSRVELYKPLNLYGESKHEFDKWALAQKAS
                     PPYWAGMKFFNVYGPNEYHKGRMASVIFHAFHQIRETGRVRLFRSHRPDFKDGGQLRD
                     FIFVRDVVDVLFFLMKRGGHSGIYNLGTGKARTFLDLVRAVFHALDKPENIEFIDTPE
                     DIRDKYQYFTEAPMAKLRAEGFEEDFYSLEDGIEEYVVEFLMNDRYF"
     gene            complement(7226..7543)
                     /locus_tag="PKZ74_05735"
     CDS             complement(7226..7543)
                     /locus_tag="PKZ74_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPM92293.1"
                     /translation="MAEKLYYSIGEVAEMFQVNASLIRFWEKEFPIIHPQKNRKGNRM
                     FTSGDIDHLRIIYNLVKERGFTIQGARDYLSKNRSEAEKNHEVVKSLQNIRSLLVRLG
                     DEL"
     gene            complement(7555..8529)
                     /locus_tag="PKZ74_05740"
     CDS             complement(7555..8529)
                     /locus_tag="PKZ74_05740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM92294.1"
                     /translation="MGKKVKYVFNHKTLSFEHARLTARHYILKVLSYLSTSVAFTAVI
                     VLIGTYFFDSPKERMLKREIRQYELQFKLINERMAKLETVLNDMADRDDNIYRVIFES
                     EPIPTEARKAGIGGSDRYRDLEGYRNSEILVKTARKLDQITAKMYVQSKSFDEVYEMA
                     INKSRLLAGIPAIQPVSNQDLRRLSSYFGWRTDPFYKVQKFHEGVDFSAPPGTEIYAT
                     GDGKVLQADRSKTGYGNQIVIDHGFGYKTMYAHLQSFKVRAGETVKRGQVIGTLGSTG
                     KSTSPHLHYEVWKNNTPVNPINYFFNDITPEQYEEMLILSQRPSQTMD"
     gene            8664..>11275
                     /gene="alaS"
                     /locus_tag="PKZ74_05745"
     CDS             8664..>11275
                     /gene="alaS"
                     /locus_tag="PKZ74_05745"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPM92295.1"
                     /translation="MKSAEIRKIFLEFFSSKSHKIVPSAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGHAPAKHLRIADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWELLTDVYKIDKNNLYVTVFEGDAADNLPKDEEAALIWSRFVPSDHILTGS
                     KKDNFWEMGETGPCGPCSEIHVDIRSEEEKAAVPGRELVNKGNPLVIEIWNLVFIEFN
                     RQMDGKLVPLPQKHVDTGMGFERLSMVIQGKKSNYDSDIFQPVIGEIAKLSGRVYGQN
                     EKEDIAMRVIADHLRAISFAIADGQLPSNTGAGYVIRRILRRAVRYGFTFLDMKEPFI
                     YRLVPVLASHMGEYFPELYSQGDLVRKVVAEEEQSFLRTLSHGIRRFEEYVKENPGRN
                     EIEGKFAFELFDTYGFPVDLTQLMASELGLHVDMPGFQAGLEEQKNRSRQAAQVDTHD
                     WTVLKPDGEETEFIGYDALDAEVEVVKFRKVRAKGQEYVEIVLDRTPFYAESGGQVGD
                     RGYLESDGHRIMIEDTYKENELIIHKASEAPGSTGKPYRAVVDNEKRRATANNHSATH
                     LMHAALRTVLGSHVEQKGSLVDEDHLRFDFSHFAKMSDEEIRRVEEIVNRKIRENIEK
                     SEERSVPVEEAKKRGAMALFGEKYGDEVRVITFDPDYSIELCGGTHVHSTGQIGLFKI
                     VSEGAIAAGVRRIEAVTGEKAEAWFYEQADTVTAVRELLKNAKDVVKGVKNLLDENKD
                     LQHRIEELNRYRIQALKSELYDNAEVIVDTRFIAASLDIDPKSAKDLSFMLHAMPGSM
                     FILVFTTGGEKVSMSLMLTEDLVKGKGLHAGNMVKELAREIGGGGGGQPHYATAGGTH
                     ANGIKSAIEKARAML"
BASE COUNT         2899 a         2534 c         3050 g         2792 t
ORIGIN      
        1 tgtaagtgat tttagctttt taggtacaga aggttatgat ggcgtttatt tgtcggcctt
       61 tgatctggct gcgaatcaga cctttgccta tcagtacgac aatacgattc cggactgggt
      121 taatctttca gatgaaacta ctcctatacc gatgtcagct ggattggcaa tcagcacaac
      181 caccaataat ccacagttgt taagtatgac gggtaaaatg atacatggtg cctatgatac
      241 tgataatggc ggtagcgggc cctctgttgt aaacctggga tggaatttga ttgcaaatcc
      301 atatccaagt ggattaggcg tttatgaagt acttgatgaa aatgccggag taattgatga
      361 agatatttat gtttgggaag gtcttaacaa tgcagaaggg ggcaactatt cagcctataa
      421 ttatactgct ggcgaaggta caggtggttt atttgatgga ttgatgggca tagggcaagg
      481 atttttcatc aatgctctca gcacacctgt gtcttttact aataacaatg ttcgttttca
      541 caccagcgag gttttgctga aaaacaatca actgtataaa ctcagattat cggcatctgg
      601 aaatgggttt aaggatgaat tcgtagtgca ttttagtgaa gcgggtagtg acagttacgg
      661 acccggcgac tccgacaaat ggcccagtat gtatggtaat gcaacggaag cctggactgt
      721 tagccctgat aatgtaaacc tgaccattca gactttatct ccgttaggag aaaatatggt
      781 gtctgttcct acttcattta aatgcggagc ttcaggaaca tacactatca acgcttcgaa
      841 ttttgaaagt tttgatgtcg gtaccgaaat ttacctggaa gatctgcagg ttggtggcga
      901 ttggcacaat ctgggagtta atcctgttta taccttctca gctaccccgg atgatgcaca
      961 ggcaagattt gttttacatt tcttcgggcc gacttcagtc gatgatccgg acgatgagat
     1021 ggccgatgta aaagatgtca gaatctacgg ttacgggcac gatgcctata tcgtcaactt
     1081 cggcagcgaa acggtgaaag aatttgtggc ctatgacctg atgggacgtg aactgcatcg
     1141 cggtaccctg cccaacaact cggtcaataa ggtccagatc ggcgaagtct ctgcctacta
     1201 tatcgtgaag gtcattacca aagaaggcaa tgtgtatacc ggtaaggttt atatcacgaa
     1261 gtaaaaacgg acaattatta atataattaa cgaaaaaaac attccagata tgaaaaaagc
     1321 aatagttaac ctgattctcg tgggattatt gataattgtt cctgtgatca tcgtcagcgc
     1381 ctcagccccc ccgccaccac caccaggacc gggaggaacc gacgacccca tcggaggcgg
     1441 agcagctccc atcggcagtg gtatcgtgat gctgatctca atgggagcag cctacggcgc
     1501 aaagaaagtc tacgacgccc ggaaaaggct cgaagaataa ggacccaaag tcatttgtat
     1561 aatacgaggg cgtctctgtt gagatcgccc tctttttgtt gcttatcgca caatgacttt
     1621 gacgaacaaa taacgctgct ttatcgtcag acggataagg taaaggcccg atgaataacc
     1681 ggaaaggtct agggtgatca atggataggt ctgctctgtg aatgtctgct caaacacctt
     1741 caacccggga atcgtaaaaa cctctgcttt aatatccgag ggcagttcct cggtaatgta
     1801 gatgctcacc agcccgccgg tgggattggg atacactttt ccgacaatat ggatgtaatc
     1861 ctttaggtac aaagtgtcgt acgctacacc gtcggtcacc gtcagcctta catcaaactt
     1921 gccgccattg gggtaattga ccgtaacagg gccatccccg ttgtaggttt ccggatcgcc
     1981 cccctcgaaa taccactccc aggccatcgg atcgccggaa ctgtaatcgg tgaaaatgac
     2041 gtcgttgcca atcaatgtta ttaccggatc ggcgttgaat gcggctgtta gctgggtgaa
     2101 ttttcccgga aggaatgaaa tgcctgtgtt atcaggatca agccagtatt ttagttgctg
     2161 agaggaatcg gtgccgttct ggtcccagga gtatgagaac ttgccgtaga agtcgggttg
     2221 atcggggcct gtattcggtc cgccgccacc cggttcgcag gccgcctgcc caccggtgag
     2281 ggtgccgatg atacggccgt aattgtcgaa cagcggcgat cccgacgatc cgccttcggt
     2341 cacaccccag ccgctttcgg tagcgctcca gtaaacctgc cagtgcgtgc cgaattcacc
     2401 gctccactgc gaagaggtaa gctggtcggt ataggtcgaa atctttttca catctcctgc
     2461 cgggtggtgt atggtgaccc cgctgaagct ggccgaattc tcaatgttcc agccgttaaa
     2521 aaaaggatcg taatgcggtg gtacctcatt atcaagccgg agaagtagga aatcggaacc
     2581 ggatgtgtcg taattggcca gctttgctgc gccattcatg gtcagcagct caggcgtctg
     2641 ggaaatgttc tcacatcccg gatattcata attgaaataa aagatccatt gtgccagttc
     2701 ttccggggtc acataaggcg cacaatgatc tgccgtcagc agataaggcg tattgtcctg
     2761 ccgggtgttg ttgaccagcg aaccggtaca ccaaaaaaaa ccgccgccat ccctgacata
     2821 gatccggacc actccctgct tctgaaactg ccagttatca ccctccgggc aattgatatt
     2881 cacctcgcag tcgtccgatg aactccggcc ggcgattctg tcgggtaaat cgcggtaaac
     2941 gcatgatatc tccccgagat ggcatcgggc accggaaata tctgccccgg caatggcatt
     3001 gagttcgatg atcactctat ctccggtggt cagctcgatc gaaaaaatgc cctgtggttt
     3061 attattatat aaggtataag ccccaagcac cgaatttttg ccgggactgt aagcataaag
     3121 actaaatcct tccggcagga aaaaccggtc gaagtgtagc ccgatggcca gtgccccgct
     3181 gcaggttacc tccagtcgcc agatttgggt accctcatcc gtaaggaaaa catcgccgtc
     3241 gttcataaca tccagtccgg caaagaccgc gatcccaatt cgccggttca ggccattctt
     3301 ttccgattcc tgatcttcag catccaggat gcttctttcg ggcatggtca ggatatgctc
     3361 cctggctgcc gggagattgt acctgaaact ttcaggcgtg cctttttgcg aaatttgtga
     3421 gaaaaggaga aggggaaaaa gggcgataag ggtgataagg agatggggag aatgggagaa
     3481 ggggagacgg ggaggcaggg agaaggggag acagggagaa tccttgcggt ctccctgcct
     3541 taaatgctta agcaaagtaa ttaaagatat tgacattgag aattatctca gaagtgaaat
     3601 cttcttctgt accgagcggg tgggggtaga aacacggaca aaatataaac ctgacggctc
     3661 atcggtcaga tcgaatgtaa ccccctggta atcagcatga aattccgctt tgcggatgac
     3721 atttccgagc gaattgacga tttctatgtt tacagaggat tgaatgttgc caccttgtac
     3781 cggtcgtccg gtccaggctt ccgcaccttc cggcagttta acggtcaaca aaccggccgt
     3841 tgggttggga aatgccatga caccctgctg gcggttgatc tcaccgaccg agatgttgcc
     3901 gacggtgata taaccgtcct tctggatcgt attgttgcca aagaagttct tggtcctcag
     3961 ccttacatag aatgtgcctt cagttggata gacgatgttt tccgggttct gcacactgga
     4021 agttccagtt gcagcgcctt caaaggtcca ggtccattgg gtgggatttc cggtggagag
     4081 atcgaagaaa ttcaccgtgt cgccaggcag aatctccgtt ttatcggcat agaagtcgga
     4141 aaccggcgga gcgcctgcaa gtaccgtgat atagttttct ttgagttcgg tgttggatcc
     4201 tccgttattc gatgcggtca gatagacgtt gtaaacaccg gggacagcat agatgaccga
     4261 gtcaggattc tgttcgccgg atgtggccgg atcgccgcca ttgaagatcc agtcccaggc
     4321 tgtcgggttg ttggtcgaca ggtcggtaaa gtcgatcata tcgccttcgg tcacttcgag
     4381 ggcgctggcc acgaagtcag catccggcag gaggacctgt tcgaccgtga tatacccggg
     4441 tttcagttcc gtttcttcgc catcggcatt ggtaacggtc agggttacat cgaatgtgcc
     4501 ggcttgatta taggtaatag tcgggttctg tatatcggag gcattcgggg taccgccggg
     4561 aaacgaccat tcccaggaga gggcggggtt tccggtggtc aggtcggtaa agtggacact
     4621 ttccccttgc aggaggtagg ttgtatctgc ttcaaaatca acaaccgggg ggagtatttg
     4681 cgacgaaagg ccggggtggg tataaacacc ggtattggtc gggtcgagcc agtctttcag
     4741 gcgttctgcc gggacatcac cgttctggtc ccatgcgtac cagattttcc cgaaccatgc
     4801 cggagacggg ttagtgcagg aattggaaga ataaccgccc gacaggtcgc ccatgatcag
     4861 gccattttcg tcgaatacgg acgagccgga agagccgccc tgcatgattg acagtccgct
     4921 ctcggtttca atccaggtga ctttccaatg cgacggggtg ccgttccacc aggtggaaga
     4981 gaccatattc tgataagtag agactttttt gatgtcgccg gcggggtgat gcaggcaaac
     5041 gccgctgtcg gccgcgttgt tggtacggtt ccatccgttg taatagacat tgtaacctga
     5101 gggaggagtc ccgttcagtt tcaccagttc gaaatcggcg ccaaggtatt cattgtcgtt
     5161 gggatcgttg gctttcagcg tgcaaccggt catggtattg ctcgaagggc cataattgcc
     5221 ggtgcaggta gccgactggt aattgaagta gaaaacccac tggttgcggt cggaggcatt
     5281 ggcaccgtcg ccgcagtgtt cagccgtgag caggtagggg gttctgtcgt tgtttgtgtt
     5341 gttgatcagt gaaccggagc accagtaata actccagccg atcttgatcg aaagccgggc
     5401 aactccctgg atctgatttt cccagccatt gccttccggg catttgacat tgatcatgca
     5461 ggaccatgac tgatcccggt ttccattttc aacaaaatcg atataacgat aagcataagc
     5521 tatttctgat ataatgacgg atacgtcgcc gcttgttccg gccggttcca ggtattcgat
     5581 attgaccagg tcgccctgga tgaattcgtt ggcaaagagg cgcgattcgt ggttattggc
     5641 atccgtgaag gccccgatga cctgggtttg cgcttcatta taaaggaaga gttttgcgcc
     5701 atccggcagg tgaaaatcac tgtagtagac tccaagtgcc agcgctcccg ggatatagac
     5761 cgccatccgg cgaagtatgg aaccatcagc caggatttca acggtgccgg cattcctgac
     5821 atcgagattg acaggcaccg aataggccat tcgctcgggg ccgggaaagg cgctccgtaa
     5881 attttgctca tcttctgcca tcaatatttc catatcaggc gccggataga ccaggcgatc
     5941 gacctgtgaa gaaagcccct gaagcgagaa actcggggga aggccacctt cgcttacctg
     6001 ggcgaatgaa gtaaaagctg taaggaacag ggttggcaaa agaagcaaac caaagcgtaa
     6061 acaattcatt ttcatcgtaa aaaaattaat aattattgta agaagatcat tataatcatc
     6121 cgggttaacg atgacaaata tagataaaat caggtgagac ggaagtaaaa aattgattcc
     6181 ggtgagcaat taaaggaatg aataatcgta tatttgatta aacaatgatt tcacggatat
     6241 gggcaaaata gtcatcactg gcgcagcagg ttttattggg agttgcatgg ccggaaaact
     6301 caaccacaaa ggctaccggg agattgttct ggttgacaat tttacaaatc cacgaaaaca
     6361 aaagaactat tgtcataaat caattgctga aaaagttgag cgcgatgact tcttcgcctg
     6421 gctgaaacag catgcggatg aggtggaatt tgtgtttcat cttggcgcca ggaccgatac
     6481 aacggagacg aatccggagg tttttgaccg gttaaacctg ggatttacca gggatgtctg
     6541 gaagttatgc acagagcaga ggatcagttt aatctacgct tcatctgccg ctacatatgg
     6601 cgggggtgag catggataca gcgatgacca cagccgggtg gaactgtata aaccgctgaa
     6661 tctgtatgga gaatcgaagc atgaatttga taaatgggca ttggcgcaga aagcgtcgcc
     6721 tccttactgg gcaggaatga agttcttcaa cgtttacggt ccgaacgagt accataaggg
     6781 gaggatggct tcggtgatat tccatgcttt tcaccagatc agggagaccg gcagggtcag
     6841 gctgttccgt tcacaccgtc ccgatttcaa ggatggaggc cagttacggg attttatctt
     6901 tgtcagggat gtggtggatg tattattttt cctgatgaaa cggggtgggc acagcggaat
     6961 ttataacctg gggaccggaa aggcacgcac attcctggat cttgtccggg cggtatttca
     7021 tgccctggat aaaccggaaa atatcgaatt cattgataca ccggaagata tccgggataa
     7081 ataccagtat ttcacggaag caccgatggc taaattgcgg gcggagggct ttgaagagga
     7141 tttttattcg ctggaggatg gcattgaaga gtatgtggtc gagttcctta tgaacgaccg
     7201 gtatttctga tggaagcagt atggatcaaa gttcgtcgcc gagcctgacc aggagcgacc
     7261 ggatattctg aagcgatttc accacttcat ggtttttttc ggcttcgctc ctgttttttg
     7321 acaggtagtc gcgtgccccc tggatcgtaa aacctctttc tttcaccagg ttgtagatta
     7381 tccgcaaatg atcgatatca ccggaagtaa acatgcggtt tcctttccgg ttcttctgcg
     7441 gatggatgat gggaaattcc ttttcccaga aacggataag ggatgcgttc acctggaaca
     7501 tttccgctac ttcaccgatg ctataatata gtttttcagc caatgcgatt gattttaatc
     7561 catggtttga gaaggccgct gggagaggat cagcatctct tcgtactgct cgggagttat
     7621 gtcgttgaaa aagtaattga tcggattgac cggtgtgtta tttttccaga cctcgtaatg
     7681 gaggtgtggt gatgtcgatt ttccggtact tcccagggtg ccgataacct ggcctctttt
     7741 aacggtttcg ccggccctga ccttgaacga ttgcaagtgc gcatacatgg ttttataccc
     7801 gaaaccgtga tcgatcacga tctggttacc gtagcccgtt ttggaacggt cggcctggag
     7861 caccttgcca tcgcccgtag catagatttc ggtccccgga ggagccgaaa aatcaacgcc
     7921 ttcgtgaaac ttctgcactt tgtagaaagg atcggtcctc cagccaaaat aagaggataa
     7981 ccggcgaaga tcctggttgc tgaccggttg aatcgcagga atgccggcca gcagccttga
     8041 tttgttgatg gccatttcgt atacctcatc aaatgacttc gactgaacgt acattttcgc
     8101 cgtgatctgg tcaagcttcc gggccgtttt taccaggatt tccgaatttc tgtagccttc
     8161 caggtccctg taccggtccg aaccgccgat acccgctttg cgtgcttccg tcggaatcgg
     8221 ttccgattcg aagatgacgc ggtagatgtt atcgtcgcgg tcggccatat cattcagcac
     8281 ggtttcaagc ttcgccatcc gctcattgat gagtttaaac tgaagttcat actgccggat
     8341 ctcacgcttg agcatccttt ctttcggaga atcaaaaaaa taggtaccga tcaggacgat
     8401 cacggccgtg aaggccacac tggtggacag ataggaaagg actttgagga tataatgcct
     8461 ggcagtgagc cgggcatgct cgaacgacaa tgttttatgg ttgaatacgt atttgacttt
     8521 ctttcccatc taatcaatgg cttccgcagg tatgaacgaa tattcttcca ttttccgtat
     8581 ttgaacggag tgcaaaaata aataaaatta attttgcagt tcgtttttaa aagcgtatta
     8641 acaggcaaat aatcagaaaa ttaatgaaat cagcagaaat aagaaagata tttttggaat
     8701 ttttctcttc gaaatcacat aagattgttc cgtcggctcc aatggtcgtt aagaacgatc
     8761 cgaccctgat gtttaccaat gcagggatga accagttcaa ggatattttt ctcgggcatg
     8821 ccccggcaaa gcatttgaga atagccgata cacagaaatg tctgcgtgta tcgggaaagc
     8881 ataatgacct cgaagaggtc ggtcacgaca cctaccacca caccatgttt gagatgctgg
     8941 gcaactggtc gttcggggat tatttcaaga aagaggcgat tgaatgggca tgggagcttc
     9001 taacggatgt ttataaaatt gataagaata acctttatgt gactgttttt gaaggagatg
     9061 ctgctgataa cctgccgaaa gatgaagagg ctgctttgat ctggagccgg tttgtgcctt
     9121 ctgatcatat ccttacagga tctaaaaagg ataatttctg ggagatgggg gagacaggtc
     9181 cctgcggccc ctgctcggaa attcatgtgg atatccgcag cgaagaagaa aaagcggcgg
     9241 tgcccggaag agagctggtc aataaaggca atccgcttgt cattgagatc tggaacctgg
     9301 tttttatcga gtttaaccgg cagatggacg gaaagctggt cccgcttccg cagaaacatg
     9361 tcgataccgg gatgggattc gaacggctgt cgatggtcat ccaggggaaa aagtcgaatt
     9421 atgattccga tattttccag ccggtcatcg gagagattgc caaactttca ggaagggtat
     9481 acgggcaaaa tgagaaggaa gacattgcca tgcgggtgat cgccgaccac ttgcgggcga
     9541 tctcctttgc tattgccgac gggcagttgc cgtcgaatac cggcgccggg tatgtgatcc
     9601 gaaggatatt aagaagggcg gtcaggtacg gctttacttt cctggacatg aaggagccgt
     9661 ttatttaccg gctcgttccg gttcttgcct cacatatggg tgaatatttc ccggaactct
     9721 attcacaggg cgacctggtc cgcaaagtgg tggcagagga ggaacagtca ttcctccgga
     9781 ccctgtcgca tggtattcgc aggtttgagg aatatgtgaa agaaaacccg ggacgtaatg
     9841 agattgaagg aaaatttgct tttgaattgt tcgataccta cggtttcccg gtcgacctga
     9901 cacagctcat ggcttcagag ctgggtttac atgtggacat gccgggtttc caggcggggc
     9961 tggaagaaca aaagaaccgc tcccggcagg ccgcgcaggt agatacacac gactggacag
    10021 tattaaaacc ggatggtgaa gagaccgaat ttatcggata tgacgcactg gatgcagagg
    10081 tcgaggtggt caaattccgc aaggtgaggg caaaagggca ggagtatgtc gaaattgttc
    10141 tcgaccggac gcctttttac gcggaatcgg gagggcaggt tggtgacagg ggctacctgg
    10201 aatctgacgg ccacaggatc atgatagaag atacctacaa ggagaatgag ctgatcattc
    10261 acaaagcatc agaggcaccg ggatcaaccg gaaaacctta ccgggctgtg gtcgataacg
    10321 aaaaaaggag agcaacggcc aacaatcaca gcgcgacaca cctgatgcac gctgcattgc
    10381 ggacggttct gggaagccat gttgagcaaa aaggctccct ggtggatgag gatcatttgc
    10441 ggttcgattt ttcccatttt gccaaaatgt cggatgaaga aattcgcagg gtggaagaga
    10501 tcgttaaccg gaagatccgt gaaaatattg aaaagagcga agagcggtca gtgcccgttg
    10561 aagaggcaaa gaagcgaggg gcgatggccc ttttcggaga aaaatacggc gacgaagtga
    10621 gggtgataac gtttgatccg gattattcga ttgaattgtg cggggggaca catgttcata
    10681 gcaccggaca gatcggtttg ttcaagatcg tttcggaagg ggccatcgca gccggagtgc
    10741 gccgtatcga ggcggtaacc ggtgaaaagg ccgaagcctg gttctacgag caggccgata
    10801 cggtgacggc cgtcagagaa cttcttaaga acgccaaaga tgtagtaaaa ggggtgaaaa
    10861 acctcctgga cgaaaacaaa gatctccagc accggatcga agaactgaac cggtacagga
    10921 tccaggcttt gaagagtgaa ttgtacgata atgcagaagt gatcgtcgac acccggttta
    10981 tagctgccag cctggatatt gatcccaaat cggccaaaga cctgtcgttc atgctccatg
    11041 ccatgcccgg aagcatgttc atccttgtat ttacaacggg tggtgaaaag gtcagcatga
    11101 gcctgatgct gacagaagac cttgtcaaag ggaaaggcct tcacgccggc aatatggtga
    11161 aggaactggc cagagagatc ggcggcggcg gtggaggaca gccgcattac gctacggccg
    11221 gcggtaccca tgccaacggg attaaatcag ctattgagaa agccagggct atgct
//
LOCUS       DAOPNH010000080        11270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6134_length_11270_cov_7.077664, whole genome shotgun sequence.
ACCESSION   DAOPNH010000080 DAOPNH010000000
VERSION     DAOPNH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6134_length_11270_cov_7.077664"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(665..2110)
                     /locus_tag="PKZ74_05750"
     CDS             complement(665..2110)
                     /locus_tag="PKZ74_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPM92296.1"
                     /translation="MTERAKLIWVYGASAVFIAANAYLFLHDKMWGLAIPFVLLLLIL
                     YIFSLDNVLLLITFLTPLAVNIKDAEFGIGISLPSEPLMFGVMLLFILKLMHERFYDA
                     KIMKHPVSIAILINLVWLFITCFTSELPIVSIKFMVARLWFIIPFYFAGILLFTDVKN
                     IRKFQWLYIIPLIIVIFYTLYNHSTYGFDKPHSNMVMKPFYNDHTAYGAIIAMFLPVF
                     VGFSLSREYRRPYRIMSFIVLAILVIGIIFSYSRAAWISVAAALGVFLLIRFRVRFYW
                     ILLTVAVGVALFFMFRYQFLDKLEKNKQDSSTDFVEHVQSISNISSDASNLERINRWA
                     AAIRLFKEKPIFGWGPGTYQFVYAPYQRAKEKTIISTNYGDMGNAHSEYIGPLSESGV
                     LGMITFIGIVVTVIYTAIRVYRRSKVKEIRLLSLVMALGLISYYVHGIMNNFLDTDKA
                     SVPFWAFTAVIVALDLYHRQEEEKSALKSTD"
     gene            complement(2107..3090)
                     /locus_tag="PKZ74_05755"
     CDS             complement(2107..3090)
                     /locus_tag="PKZ74_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92297.1"
                     /translation="MERYFNNTNLINLLLKWRIHLLVILALAVVLSVFFSGPLFITPK
                     FKSFGVIYPANVSPYSEESETEQMFQVLQSQDIIDSVINKFNLAEHYEIAKEYKYYQT
                     TIYYEYSQNIKIEKTPYDAVRIEALDKDPVIASQIVEAIIGFYNAKVRYMHNEKYYEV
                     MDMYEKLLARKQSDIDSLKASLYKLMVESGLLGWDQSSEEIMRGYLRTVTGGAAPQIN
                     TAEVKRLKENMEKRGGDLILITERIKHEANSYSAFKVEYEDAMRFFNASLTYCNVVTK
                     PFPADKKSYPVRWLIVAMTFILTFFFAVVVILLVENLRVYQLRKNQNKDQS"
     gene            complement(3129..4190)
                     /locus_tag="PKZ74_05760"
     CDS             complement(3129..4190)
                     /locus_tag="PKZ74_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92298.1"
                     /translation="MTDPNNQESLKKDLNSLNLLHLLFKWRLPLFIITAVAIGAAVLF
                     SSPFFIKPKYKSSVIMFPVATNSISKVLISQNSGIKEDILGIGEEEQAEQMLQMLNSN
                     FIRDRIVQKYNLFKHYRIPENSKHKYTRLFREYENNVRFRRTEYMAVKITVFDTDPQI
                     AADMANDIAALLDSTRNMIQKERAVKAFRIVDEEYRSLENEVNGIVNQLKYLGSKGVN
                     DYERQSEVLNQQLAIALAQNNRHAITILQQKLDTIGKYGGEFLSLKNSLEFKTEQLTL
                     LKTKYQEAKVDAEQFLPQKFIVNSAYKAEVKSYPIRWIIVVVTAFIVFFTAVLIIIIV
                     ENYTGLQLKKNSGSRQYLS"
     gene            complement(4322..5809)
                     /locus_tag="PKZ74_05765"
     CDS             complement(4322..5809)
                     /locus_tag="PKZ74_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM92299.1"
                     /translation="MQKKFLVNLAISLFLNLLVKPFWIFGIDRTVQNTVGLADYGFYL
                     NIFNFAFLFNILLDAGITNFNNRNIAQHVQLLNKHFSSLVVLKFLLTFVYIAVTFSIA
                     LVLGYRGDQLIMLAWVGFNQFLLSFILYFRSNISGLLMFMTDSVISVLDRVLMILICA
                     VLLWGNVTGGVFRIEWFVYAQTAAYLATLVIAAVIVMRKAKFRKLSWSRPFFLVILKK
                     SLPFAVLVLLMSVYNRVDTVFIERLVGGDEGERQVGIYAHAFRLLDALNQFAWLFAVL
                     LLPIYSRMIKLKEDVGRMTRLPYALLITSAIIVAMGSLFYRFEIMEWLYPKDSMQTAA
                     EYAGQHRQSATVFGILMFGFLGSTTMYVYSTLLTANGNLRQLNLVALAGIGVNFLVNI
                     LLVPRMQATGAAVASLATQLFTAGAYMALAHRIFRFKPDIAFLSRIVIFTLVVIFLNF
                     LCRQLRVDWKISFIVMMIASALSAFVLKLVNLPEMWRMVKERATP"
     gene            5980..7116
                     /locus_tag="PKZ74_05770"
     CDS             5980..7116
                     /locus_tag="PKZ74_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPM92300.1"
                     /translation="MKIAVNTRLLLKNKLEGIGWFSFETLKRITTQHPEHQFYFIFDR
                     PFDDSFIFSDNITPVVVPPQARHPILYYLWFEKSIPRVLKKIEPDLFFSPDGYLSLST
                     PVKSMHVIHDLNFEHYPDDLPYPERTYYRHYFPQYARKACRIATVSEFSKRDIINQYH
                     IGPEKIDVVYNGANDRYSPIPDSEKTLVRAKYSQGLPYFVFVGALHPRKNLANLFRAY
                     DLFRSETGINMKLLIIGHKKWWTRQIKESYEKMRFNGDVEFCGRLDPVELNRVISSAA
                     ALTYVSYFEGFGIPILESFYCETPVITSNVTSMPEVAGDAAILVDPFSVKSISEGLTK
                     IATDPELSRTLNEKARIRRQVFHWQRTADLLWNSIEKCLDTKKS"
     gene            7128..8417
                     /gene="purD"
                     /locus_tag="PKZ74_05775"
     CDS             7128..8417
                     /gene="purD"
                     /locus_tag="PKZ74_05775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPM92301.1"
                     /translation="MNVLIVGSGGREHALAWKIRQSPLLDKLYILPGNAGTDSLGENI
                     QLSVTDFKGIGRTVLEKQIDMVVVGPEVPLVEGIHDYFLADPVLKAIPVIGPVQKAAM
                     LEGSKDFAKEFLIRHHIPTARYRTFTRETYGNALEFLREMKPPYVLKADGLAAGKGVV
                     ICSDFGEAGTELQAMLMDEKFGEAGAKVVIEEFLSGIEMSAFTLTDGRHYLVLPPAKD
                     YKKIGEGDTGPNTGGMGSVSPVPFADDAFLKKVEERIIAPTHAGLLQEGIDYRGFIFF
                     GLINVGGDPYVIEYNARMGDPEAESVIPRIKNDLLELFAAVGNQTLHHHRIETVPGFT
                     ATVMLVSKGYPGNYEKNMEITGLDGIDGSLVFHAGTAIRPADGKVVATGGRVIAVTSF
                     GDSLTEAFGKSYENADRIRFENKSLRSDLGRDLLKFA"
     gene            8465..8755
                     /locus_tag="PKZ74_05780"
     CDS             8465..8755
                     /locus_tag="PKZ74_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HPM92302.1"
                     /translation="MKAKTDKPEKARFDARLTREQKQIFERAAVIGGYRSLSDFVITI
                     AEERAQQIISKADSIIASQRDAEIFFDAVLNPGQPNEALRSAADEYNKLMAE"
     gene            8752..9258
                     /locus_tag="PKZ74_05785"
     CDS             8752..9258
                     /locus_tag="PKZ74_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM92303.1"
                     /translation="MNLLTEPLNHLHKRNDFSCGNLLLDNYLKYQAGQDTRKKLSACF
                     ILSDHTTHQIKGYYTLSNSSLPTELFPEKLKLHFPRSYISIPAILLGRLAVDKTRQGK
                     GTGRLLLIDALRRSYLVSESIGSFAVVVDAISEEAGLFYDKFGFIMLPDSGKLFMPMK
                     TVKHLFER"
     gene            9288..10280
                     /locus_tag="PKZ74_05790"
     CDS             9288..10280
                     /locus_tag="PKZ74_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92304.1"
                     /translation="MLIKFFRSSYLIQYFALALITAALWIPGFRENQGLPVSPVIIAP
                     IYGMLHYVLSLHPVASPAFAIAIILVTAFALNNILIFHELTPKNNLLPAFLFIVLMSS
                     NPHTLCSYPVLTALLFFVWFLHTIYRMNDSPDNYMEVFNASMLASVISMIYPVASLLF
                     FFIWITLLVYGTFNGRNLLISFIAFSLPYLYLFLYFFWTDGIDEALAAYVALFREIMV
                     FSMNREILQIILWALFTLFMLIPSFFRITGNLSSFGINFRKKMAATAWLLAFSIPMIV
                     MQGKVDYHTLLFLPATILIAHYFHQFKKSVLNETVLLVFILLALIGNYWPLLHA"
     gene            10273..10896
                     /locus_tag="PKZ74_05795"
     CDS             10273..10896
                     /locus_tag="PKZ74_05795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPM92305.1"
                     /translation="MLKPYCTGEFTEAGCDEAGRGCLAGPVFSAAVILPKDYRNEALN
                     DSKKLSARQREALRKVIEADAVAFAVARVDHATIDEINILNASILAMHEAVGRLSVKP
                     GLLLIDGNRFRPYPGIPHRCIVKGDAIYMSIAAASILAKTYRDEFMTGLDRQFPCYGW
                     KHNKGYPTAAHRRAIQEFGISAMHRLSFRLTPAGDLFSGSLSRDHQK"
     gene            10985..>11270
                     /locus_tag="PKZ74_05800"
     CDS             10985..>11270
                     /locus_tag="PKZ74_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92306.1"
                     /translation="MKYVILILAIFIPSGIFAQGFAPTGAIWHNSQSDWINPDLITYK
                     TIESVSDTIITGKSCKVLIEVERSYSDTAATFKHYMYSENDSVFFYKDNAF"
BASE COUNT         2829 a         2874 c         2812 g         2755 t
ORIGIN      
        1 cgtttgataa ctttttggtt tgagactcca tctggtttgc atgagatggc atctcgattg
       61 caaggagact ccatctcaaa atcgagatgg catctcataa acggagactc catctcacat
      121 cgggcagatg gagtcccggt taagatgaga ctccatctca aatccgagat ggcatctcat
      181 taaattgaga tggagtctca aagaattacc cggttaggca ttgtaacagg gtttccaacc
      241 ttttttgctc cacacaatcg aatgatatat agagacttaa gatggggaat aattttacgg
      301 atttttttcc aaaaactata tccacgaaga tcaacgtttt tcaatttatt caaagatccc
      361 aaatttcagg gttggaaacc ctgtttcctc cgcccttttt ccagggattt cgtccctggc
      421 taccatattg ttctccctgg cgggagaaat atggcgattt gacgatttgg cgatttgacg
      481 atttggcgat ttgacgattt ggcgatttga cgatttgacg atttggcgat ttggcgattt
      541 ggcgatttgg ggatatgagg atttacctgc cggagcaata caaatttcaa ccaacggctt
      601 tagtcgtcgg agcacaatca aaggcaaccg acggctttag tcgccggcct gcacaaaaac
      661 ctttttaatc tgtggatttt aaagcggatt tctcttcctc ttgccggtga tacagatcca
      721 gcgcgacaat aacggccgta aaggcccaga acggcacgga ggctttgtcg gtatcgagaa
      781 aattattcat gatcccgtga acataatacg aaatcaggcc cagcgccatc accaggctta
      841 gcaatctgat ctccttgact ttagatcgtc tgtaaacccg gattgcggta tagatgacag
      901 taacgactat cccgataaag gtaatcattc ccagcacgcc cgattcggac agcgggccga
      961 tatactcgct gtgcgcattc cccatatcgc cgtagttcgt actgatgatg gttttctctt
     1021 tggcccgctg gtaaggcgca tacacaaact ggtaggttcc cggtccccag ccaaagatgg
     1081 gcttttcctt gaaaaggcgt atcgctgcag cccaccggtt gatccgttcc aggttggaag
     1141 catccgagga gatattcgag atcgactgca cgtgttccac aaaatcggtc gaagaatcct
     1201 gcttgttttt ctccagttta tccaggaact ggtaacggaa catgaagaac aaagccacgc
     1261 cgacggcaac tgtgagaagg atccagtaaa acctgaccct gaagcggatc agcaggaaaa
     1321 cgcccagggc ggcggctacg cttatccatg ccgcacggct gtatgaaaag atgatgccga
     1381 tgaccaggat tgccagcacg ataaacgaca tgatccggta aggcctccgg tactcccggc
     1441 ttagcgaaaa accgacaaat accggcagga acatggcaat gatcgctcca taggccgtat
     1501 ggtcattata gaaaggcttc atcaccatat tgctgtgcgg cttgtcgaaa ccgtacgtgg
     1561 agtggttata gagggtataa aatataacga tgatgagggg aatgatgtat agccactgga
     1621 acttccttat atttttcaca tctgtgaata aaaggatacc ggcaaaataa aacgggatga
     1681 tgaaccagag cctggccacc atgaatttaa tcgaaacgat aggtaattca ctggtgaagc
     1741 aggtgatgaa aagccatacc aggtttataa gaatagcaat gctgaccgga tgcttcatga
     1801 tcttcgcatc gtaaaagcgc tcgtgcatca gcttcaggat gaacagcagc atgacgccga
     1861 acatcagggg ctctgaagga agcgatatac ctattccgaa ctcggcgtct ttgatgttga
     1921 ctgccagcgg ggtcaggaaa gtaatgagca gcaggacatt atcgagcgaa aagatataga
     1981 ggataagcag gagcagaacg aaagggatag ccagccccca catcttatca tgcaggaaaa
     2041 ggtaggcgtt tgccgctata aagaccgcgc tggcgccgta cacccagatt aatttggccc
     2101 tttcggtcaa ctctgatctt tgttttgatt ctttcttaac tggtaaacgc ggaggttttc
     2161 aacgagcagg ataaccacca cggcaaagaa aaatgtaagg atgaatgtca tggccacgat
     2221 cagccagcgt accgggtacg actttttatc ggccggaaac ggcttggtaa cgacgttgca
     2281 gtaggtcagg ctggcattga agaaccgcat ggcatcttca tattcgacct taaaagccga
     2341 ataggagttg gcttcatgtt tgatcctttc ggtgatcagg atgaggtcgc ctcctcgttt
     2401 ttccatattc tccttcagcc gtttaacctc cgcggtgttg atctgcgggg cggctccgcc
     2461 ggtaacggtg cgcaggtaac ccctcatgat ctcttccgaa ctctggtccc accccagcag
     2521 ccccgactcg accatcagtt tatagagtga tgccttcaat gaatcaatgt cgctttgttt
     2581 cctggccagc aatttttcgt acatgtccat cacttcgtag tacttctcgt tgtgcatgta
     2641 tctcactttg gcattgtaga aaccgataat ggcttcaacg atctggctgg cgataacggg
     2701 atccttgtca agggcttcaa tcctcaccgc atcgtagggg gttttctcga tcttaatatt
     2761 ctggctgtat tcgtaataga tggttgtctg gtaatattta tattctttgg caatttcgta
     2821 atgttcggca agattgaatt tattgatgac gctatcgata atatcctgtg attgaaggac
     2881 ctggaacatc tgttcggttt cgctctcttc ggaatacggc gaaacgttgg ccgggtagat
     2941 aacaccgaaa gatttgaatt ttggggtgat gaacaacggg ccggagaaga aaaccgacag
     3001 cacgactgcc agggccagga tcaccagcag gtggatccgc catttcagca gcaggttgat
     3061 caggttcgta ttattaaagt atctttccat ttttaactca gataaaatta tattctgaat
     3121 actctctttc agcttaggta ttgccgggat ccggagtttt tttttaactg taacccggtg
     3181 tagttctcga cgatgatgat gatcagtacc gcggtgaaga agacgataaa agccgtcacg
     3241 accactatta tccaccgtat cgggtacgat ttgacttccg ctttgtaggc gctgttaacg
     3301 ataaacttct gcggcaggaa ctgttccgca tccaccttgg cttcctggta ttttgttttt
     3361 agcagggtta gttgctccgt tttaaattcg agggaatttt tcagcgacag gaactcgccg
     3421 ccgtattttc cgatcgtatc cagtttctgc tgcaatatcg taatggcatg ccggttattc
     3481 tgtgccaggg cgatagccag ttgctggtta agcacctccg actgccgctc ataatcgttc
     3541 acccctttgc tgccaaggta cttcaactga ttgacgatcc cgttgacttc gttttcgagg
     3601 ctccggtatt cttcgtcgac gatccggaaa gctttgacgg cgcgttcttt ctggatcata
     3661 ttgcgggtgg agtcgagcaa agcggcgata tcgttggcca tatcggcggc tatttgcgga
     3721 tcggtatcga atacggtgat tttcactgcc atatattccg tccggcggaa cctcacgttg
     3781 ttttcgtatt cacggaacag gcgggtatat ttatgctttg aattttccgg aatacggtaa
     3841 tgtttgaaca ggttgtattt ctgcacgatc cggtcgcgga tgaagttgga gttgagcatc
     3901 tggagcatct gttcggcctg ttcctcctct cctatcccga ggatatcctc ttttattcct
     3961 gagttctggc tgatcaggac tttggagatg gagttggtgg cgaccgggaa catgatcacc
     4021 gatgatttgt atttcggttt gatgaagaaa ggcgatgaaa agaggacggc ggcaccgatg
     4081 gcgacggccg taatgatgaa cagcggcaat ctccacttga aaagcaggtg aaggaggtta
     4141 agggaattca ggtctttttt cagcgattcc tggttattcg gatcggtcat gttcagcttt
     4201 ttccaaaaaa gaggtcaaat gtaggaataa atatttaatg atgatgggtt atcagaaagt
     4261 taagtgaaaa aagataattg gtgcaataac agcatactcc gcctgttcgt ctgttcgcct
     4321 gttatggtgt tgccctctct ttcaccatcc gccacatctc cggaaggttg accagtttaa
     4381 ggacgaaagc ggaaagggcc gatgcaatca tcataacgat gaagctgatc ttccagtcga
     4441 cccggagctg ccggcaaagg aaattcagga aaatgacgac gagggtaaag atgacaatcc
     4501 gggaaaggaa agctatatcc ggcttaaaac ggaaaatgcg gtgggccagg gccatgtaag
     4561 cccccgcggt gaacaactgg gttgccaggc ttgctactgc cgcgccggtg gcctgcatcc
     4621 ggggcacaag caaaatattg accaggaagt tcaccccaat acccgccagc gcaaccaggt
     4681 tgagctgccg gaggttgccg ttggccgtca gcagggtgct gtaaacatac atcgtggtgc
     4741 ttcccaggaa accgaacatc aggatgccga aaacggttgc cgactggcgg tgctgacctg
     4801 cgtactcagc ggctgtctgc atactgtctt tcggatacag ccattccatg atctcaaacc
     4861 ggtaaaacaa cgaacccatt gccacaatga tcgccgaggt gatcagcagc gcatacggca
     4921 gccgggtcat ccggcccaca tcctccttca gtttgatcat cctcgaataa atgggtaaca
     4981 acagcacggc aaagagccag gcaaactggt tcagcgcgtc gagcaaacgg aatgcgtggg
     5041 catagatccc cacctgccgt tccccttcat ccccgcccac aagccgctcg atgaaaaccg
     5101 tgtcgaccct gttgtagacc gacatgagca gcaccagcac ggcgaacggc aggcttttct
     5161 tcaggatcac caggaaaaaa gggcggctcc agctcagctt ccggaacttc gccttccgca
     5221 tcacgatgac cgccgcaata accagcgtgg ccaggtaggc cgccgtctgg gcatacacga
     5281 accactcgat ccggaacacg ccgccggtca catttcccca gagcagcacg gcacagatca
     5341 ggatcatcag cacccggtcg agcaccgaaa tcacactgtc ggtcataaac atcagcagtc
     5401 ccgaaatatt cgaccggaaa tagaggatga acgacagcag gaactggtta aaccccaccc
     5461 aggccagcat gatgagctga tcgccgcggt accccaggac cagggcaatg gaaaaagtta
     5521 ccgcaatata aacaaaggtc agcaggaact tcagcaccac caggctggag aaatgcttgt
     5581 ttagcaactg aacgtgctgg gcaatattgc ggttgttaaa attagtgatg cccgcatcaa
     5641 gcaaaatatt gaacaggaaa gcgaagttga aaatgttcag gtaaaatccg taatcggcca
     5701 gcccaacggt gttctgcacc gtccggtcga tcccgaaaat ccagaacggc ttgacgagca
     5761 gatttaaaaa cagcgaaatg gcgaggttga ccaggaattt cttctgcatc gcagcttgtt
     5821 tgaaacttct gaaaagtacc ggcggttttg gctgcccact ttttagccct ccttttcaaa
     5881 ataaattatt tttgtacaaa gttatgagaa ttaaagaacg atgaacaggc cagaaccgat
     5941 atgttccggc cgggtttatt tccaacaaac cacacagttt tgaaaatcgc ggttaacacg
     6001 aggttactcc ttaaaaacaa gctggaaggg ataggatggt tttcctttga aacccttaaa
     6061 aggatcacga cgcaacaccc cgaacatcag ttttacttca ttttcgaccg tccgttcgac
     6121 gattcgttca ttttttcgga taacatcact cctgtcgtcg ttcccccgca ggccaggcat
     6181 ccgatcctgt attacctctg gttcgagaaa tcgatcccgc gcgttctgaa gaaaatcgag
     6241 cccgatctgt tcttttcacc cgatggctat ttgtcacttt caaccccggt aaaatcgatg
     6301 catgtcatcc atgacctgaa cttcgaacat taccctgacg acctgccata ccccgagcgg
     6361 acgtattacc ggcactattt cccgcaatac gcccggaaag cctgccggat cgccacggtt
     6421 tccgaattct ccaagcgcga tattatcaac cagtaccaca tcgggcccga aaaaatcgac
     6481 gtggtttaca acggcgccaa cgaccgctac tccccgatcc ccgattcgga aaaaacgctg
     6541 gtcagggcaa aatattcaca gggccttccc tatttcgttt ttgtgggcgc ccttcatccg
     6601 cggaaaaacc tggccaacct gttccgggct tacgacctgt tccgttccga aacgggcatc
     6661 aatatgaagc tgctgatcat cggccataaa aaatggtgga cacgtcagat aaaagaatcc
     6721 tatgagaaaa tgaggttcaa cggcgatgtg gaattttgcg gcaggctcga tccggtggaa
     6781 ctgaaccggg tcatctcctc ggcagcggcg ctgacctatg tctcctattt tgaaggtttc
     6841 ggcatcccga tcctggaatc gttctactgc gagacgccgg tgatcacctc caacgtcacc
     6901 tcgatgcccg aggtggccgg cgatgcggcc atcctggtcg atccgttctc ggtgaaatcg
     6961 atcagtgaag ggttgactaa gatcgccacc gatccggagc tgagccgcac actgaacgag
     7021 aaagcgcgca tccgccggca ggtattccac tggcagcgca ccgccgacct gctctggaat
     7081 tccatcgaaa aatgcctcga caccaaaaaa tcctaaaccc tgtaaagatg aacgtattga
     7141 tcgtaggatc gggcggacgt gaacatgcgc tggcctggaa gatcaggcaa agcccgctcc
     7201 ttgacaaatt gtacatcctc cccggcaatg cgggtaccga cagcctcgga gaaaatatcc
     7261 aactctccgt aaccgatttc aagggcatcg gccgcaccgt tctggagaag caaatcgaca
     7321 tggtggtggt gggcccggaa gtcccgctgg tagaagggat ccacgactat ttcctggccg
     7381 atcccgtctt aaaggccatc cccgtgatcg ggccggtgca aaaggcggcc atgctcgaag
     7441 ggagcaagga tttcgccaag gaattcctca tccgtcatca tatcccgacg gcccggtacc
     7501 ggacctttac ccgggagacc tacggcaatg cgctggaatt cctccgggag atgaaaccgc
     7561 cctatgtgct gaaagccgac ggcctcgccg ccggcaaagg ggtggtgatc tgcagcgatt
     7621 tcggggaagc cggaacggaa ctgcaggcga tgctgatgga tgaaaaattc ggcgaggccg
     7681 gcgcaaaggt agtgatcgaa gaattccttt ccgggataga aatgtcggcc tttacgctga
     7741 ccgacggccg gcactacctg gtgctccccc cggccaagga ttacaagaag atcggcgaag
     7801 gcgacaccgg gccgaataca ggcgggatgg gatcggtctc ccctgtgcct ttcgccgacg
     7861 atgctttcct gaaaaaagtg gaagaacgga tcatcgcccc gacccacgcc ggtttgctac
     7921 aagagggcat cgattaccgc gggttcatct ttttcggcct gatcaacgtc ggtggagatc
     7981 cttacgttat tgagtacaat gcccggatgg gtgatccgga ggccgaatcg gtcatcccca
     8041 ggatcaaaaa cgacctgctg gaactctttg cggcggttgg aaaccaaacg cttcaccatc
     8101 accggatcga gaccgttccc ggatttacgg ccacggtcat gctggtgtcg aaaggctacc
     8161 cggggaacta tgaaaaaaac atggaaatca ccggtctcga cgggatcgac ggcagccttg
     8221 tttttcatgc aggcaccgcc atccggccag ccgatggaaa agtggtcgct accgggggaa
     8281 gggtcattgc cgtgacctct ttcggcgatt cgctgacgga agcattcgga aagtcgtacg
     8341 aaaacgctga caggatcagg tttgaaaaca agagcctcag aagcgacctg gggcgggatt
     8401 tgctaaaatt tgcctgaatc cggaaatgta cggatatatt acgtacattt gcaaaaaaat
     8461 tatcatgaaa gcaaaaacgg ataagcccga aaaggccagg ttcgatgcac ggctgaccag
     8521 ggagcaaaag caaatctttg agagggctgc ggtaatcgga ggttacagaa gcctttccga
     8581 ttttgtcata acgatagcag aggaaagggc acagcagata atcagtaagg ccgacagcat
     8641 catcgcttcg caaagggatg ctgaaatctt ttttgacgct gttctcaatc ccggccagcc
     8701 caatgaggct ttaagatcgg ccgcggatga atacaataag ttgatggccg aatgaacctc
     8761 ctgacggaac cgctgaacca tctccacaaa aggaatgatt tttcatgtgg taatctcctg
     8821 ctcgacaatt atttaaagta ccaggccggc caggacacgc ggaagaaact gtcagcctgt
     8881 tttatcctga gtgatcacac cacccatcaa attaaaggat actacacact ttcaaacagc
     8941 agcctcccca cagagctatt tcctgaaaaa ttgaagcttc actttcccag gtcctacatt
     9001 tcaatacctg ccatactgct gggtcgcttg gccgtcgata aaacccggca gggaaaagga
     9061 accggccggt tgcttttaat tgatgcactc cggagaagtt acctggtctc ggaatcaata
     9121 ggttcatttg cagtagttgt tgatgctatt tcggaagagg cagggctatt ttatgataaa
     9181 ttcgggttta tcatgcttcc ggatagcggt aaattgttca tgcccatgaa aactgtcaag
     9241 catttatttg aaagataatc ctgatatttg gtaaaagttt aatcgtaatg ctgataaaat
     9301 tcttccggtc gagctatctt atccagtact ttgcgctggc gctgatcact gcggcgctct
     9361 ggatccccgg cttcagggag aaccaggggc tgcccgtttc acctgtcatc atcgccccca
     9421 tctacgggat gctgcactac gtgctttcgc tgcatcccgt cgccagtccg gcctttgcca
     9481 tcgccatcat cctggtcacc gccttcgccc tgaataacat tctcatcttc catgagctga
     9541 cgccgaagaa taacctgctg cccgctttcc tgttcatcgt gctgatgagc agcaacccgc
     9601 atacgctttg ctcctacccg gtactcacgg cattgctctt tttcgtctgg ttccttcata
     9661 ctatttaccg gatgaacgat tcacccgaca attacatgga ggtttttaac gccagcatgc
     9721 tggcttcggt catctcgatg atctacccgg tggcctcgct cctgttcttt ttcatctgga
     9781 tcacgctgct ggtttacgga accttcaacg ggcgcaacct gttgatatcc ttcattgcct
     9841 tctccctgcc ttatctttac ctttttctct atttcttctg gaccgacggg atcgatgaag
     9901 ccctggccgc ttacgtagcc cttttccggg agatcatggt tttcagcatg aaccgggaga
     9961 tcctgcaaat catcctgtgg gccttgttca cgctgttcat gctcatcccc tcctttttcc
    10021 ggattaccgg caatctcagc tctttcggga tcaatttcag gaagaagatg gcggctacgg
    10081 cgtggctgct ggctttcagc attcccatga tcgtcatgca ggggaaggtc gattaccaca
    10141 ccctgctgtt tctcccggcc acgatcctta tcgcacatta tttccaccag ttcaagaaat
    10201 cggttctgaa tgagaccgtg ctgcttgttt ttatcctgct ggctttgatc ggcaattact
    10261 ggcctttatt gcatgcttaa gccctattgc accggagagt tcacggaggc cggctgcgac
    10321 gaggcggggc gcggatgcct ggcgggtccc gtgttttccg cggcggtcat ccttccgaaa
    10381 gattaccgca acgaagcgct gaacgactcc aagaagcttt cggcgcgtca gcgggaagcg
    10441 ttgcgcaagg tgatcgaggc cgatgcggtc gctttcgccg tggcccgggt cgatcatgca
    10501 accatcgacg agataaacat cctgaacgcc tccatcctgg cgatgcacga ggcggtgggc
    10561 cggttatccg taaagccggg gctgctgctg atcgacggca accggttccg gccgtatccc
    10621 ggcataccgc accggtgcat cgtaaaagga gatgccatct acatgagcat agccgcggcc
    10681 agcatcctgg caaagacata ccgcgatgag tttatgaccg ggcttgaccg gcaatttccc
    10741 tgttacgggt ggaagcacaa caaagggtac ccgacggcag cgcaccggag agcgatacag
    10801 gaattcggta tatccgccat gcaccggttg agcttccggc tcaccccggc cggggatttg
    10861 ttttccggtt ccctaagccg ggatcatcaa aaatagtttc ttataagatt gcttttccct
    10921 ccggaataac atatctttat tgcgttgatt tatcacaccg cgtaagtatg tattttttta
    10981 gaaaatgaaa tacgtaattt tgattctggc catatttata ccttccggga tttttgctca
    11041 gggatttgcg ccgaccggag ccatctggca taactcccaa tcagactgga tcaatcccga
    11101 tttaatcacc tacaaaacta tcgaatcagt atcagatacc ataataaccg ggaaatcatg
    11161 caaggtcctt attgaagtgg aaagatcata ttccgacaca gcagcaacgt tcaagcatta
    11221 catgtattct gaaaacgata gcgtattctt ttacaaagac aatgcttttc
//
LOCUS       DAOPNH010000081        11234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6163_length_11234_cov_6.353699, whole genome shotgun sequence.
ACCESSION   DAOPNH010000081 DAOPNH010000000
VERSION     DAOPNH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6163_length_11234_cov_6.353699"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1233
                     /gene="gcvT"
                     /locus_tag="PKZ74_05805"
     CDS             145..1233
                     /gene="gcvT"
                     /locus_tag="PKZ74_05805"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPM92307.1"
                     /translation="MKKTVFCGLHEANGGKMVEFAGFYMPVQFEGVNAEHETVRKGVG
                     VFDVSHMGEFWIKGPKAFELVQRLTSNDVAKLDDGKIQYTCFPNDKSGIVDDLLTYRF
                     DAETYLLVVNAANIDKDWAWVVKNNIHGATIFNASDEISQLAIQGPKAQAVMQKLTDT
                     DLNTIPYYTFKKVDLAGVKQVILSNTGYTGAGGFEVYMANSDGPAIWKALMEAGAEFD
                     IKPIGLAARDTLRLEMGFCLYGNDINDTTSPLEAGLGWITKFADGNDFINRPALEKQK
                     SEGLKRMLRGFEMIDKGIPRQHYEICDENGRVIGEVTSGTMSPSLKIAIGMGYVETAH
                     SGIGNHVFIRIREKLLKAIIVKIPFYKG"
     gene            1246..1983
                     /locus_tag="PKZ74_05810"
     CDS             1246..1983
                     /locus_tag="PKZ74_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPM92308.1"
                     /translation="MMKGPVADVCFSPALLPGFEKRTDRIVVVIDILRATTAMCTAFG
                     FGVKSVIPVMKKETALARKEKGWLVAGEENGIRLPFADFGNSPREFRSKAMKGKEIVF
                     CTTNGTKAILKGRSYGQVIIASFVNLEAVCTYIKKQKRDVLILCAGWKDRFSLEDSVA
                     AGALADRLIHAYGYLPGNDAALAAISLWNQASKSLLKTVSRSDHYLRLLGIEDRKGLR
                     YCFFPEKFRVVPGLQTNKLINLPEIKE"
     gene            complement(1973..2335)
                     /locus_tag="PKZ74_05815"
     CDS             complement(1973..2335)
                     /locus_tag="PKZ74_05815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPM92309.1"
                     /translation="MRNLLKKFLIRLLKSFKYAIRGFSGVAITQPNFIIHLLAIGVVF
                     VIGYYQKITLMEWGLVVLAIGLVLIAEIFNTAVEWLGDLISPDYNEKIKWAKDAAAGG
                     VLVASGTAILIAIIVFIL"
     gene            2549..2621
                     /locus_tag="PKZ74_05820"
     tRNA            2549..2621
                     /locus_tag="PKZ74_05820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2582..2584,aa:Gly,seq:ccc)
     gene            2719..3696
                     /locus_tag="PKZ74_05825"
     CDS             2719..3696
                     /locus_tag="PKZ74_05825"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HPM92310.1"
                     /translation="MLFVFILTGSVTFAQKSNLVFYTEQGETFYLNINGIPQNYEPVS
                     NLMISDLVDQTCKVTLKFEDPALGEISKNLTFGQGSETTYVIRMNASDEWILRYVKEV
                     PVAQAPAPPADRSVIVLRTTAPAKPVKTGQSQSTANQATTTSTTSEVATEGGSVGMNV
                     QEPGGNITINFNVSGVGTSTSTSTTTSATTSTVTGNTDVQNPEPEPVAVIEKPKPVAI
                     PGYSGLIGCDWPMSEYDFSMAKNSIESKSFEDSKLTLAKQVFDSKCMLCIQVKEIMKL
                     FSFEESRLEFAKYAYGYTYDLSNFYIVNDAFSFESSIDELNKYIRSQNN"
     gene            3792..5492
                     /locus_tag="PKZ74_05830"
     CDS             3792..5492
                     /locus_tag="PKZ74_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPM92311.1"
                     /translation="MNNRRVISIIKILIWIAPLLPWSLLPGQELMPVLTHEMTPEEEL
                     RKGEIGRSFTPTSPPLAPARAIAEFEQMQSVLIRYPFGIPMSLIVEMADDCMVTTIVA
                     SQAQLDQVLNQYIAAGVDTSHCEWLIAPTNSYWTRDYGPWFVADSSHQVGICDFPYNR
                     PRPYDDNIPVVLASQMDIPLYGMNLIHTGGNWMCEGLGNASSTELVWTENPTLTHQEI
                     DDLVESFLGIDTYHVLPDPLGEYIQHIDCWGKFLDVDKVLIGQVAESDYRYDDYEYVA
                     GYFASQISAYGTPYQVFRVYTPGDYPYTPYTNSLILNKKVLVPVTGSPWDDEALGAYA
                     DAMPGYEVIGVIYSGWQNTDALHCRAIGIADTGMLYIHHMPLSGEQEFSIGYTISCSI
                     IPYTDEELISDSLLCIYKIGNGSFQTVPLASGGGSVFSASLPYIAPGSQVSYYLHAAD
                     SSGRSMSHPFIGSPDPHVFTVQNEVPGLWTGEVSGDWMNPSNWSNNAVPASPTQVVIP
                     PGSSHWPVFSGDLVVGSTCGNIIITGTAQMTVTGSVTLHPGKTITILNNGHLIQTQTP
                     "
     gene            5643..6095
                     /locus_tag="PKZ74_05835"
     CDS             5643..6095
                     /locus_tag="PKZ74_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPM92312.1"
                     /translation="MKNNLFKLLFTTIAIFANVSLFAQAPADAITGKWLNEDKDAHIE
                     VYKESGKYFGKIVWLKDPIDEVTGKPKLDDENDDEALQSRPILGLVILKDFIYDGDGE
                     WEDGTIYDPKNGSTYDCYIQFGDDKTLKIRGYIGISWIGRNTYWTRVE"
     gene            6138..7082
                     /locus_tag="PKZ74_05840"
     CDS             6138..7082
                     /locus_tag="PKZ74_05840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPM92313.1"
                     /translation="MNAIYAIGETVYDIIFRNDQPVAARPGGSMLNTAISLGRSGLKV
                     EMITELGDDPVGKLVTAFLNDNGVSTSFIQPAGNYKTPVSLAFLDDSGDARYSFYKNI
                     PEKRLNIGQAEAGKGDVVLFGSFYSLDAGIRHEIISFINKSKQNGALIFYDPNIRKNH
                     LGEINKLMPSVEENISLADIIRGSDEDFFNLFGLDDQFEIAHKVIQSGCHHMVITRGK
                     HGADYLSRDEAIHFPAIETKAVSTIGAGDAFNAGIIYAIIKNDIPGIELVNINSATWK
                     TILRIGTIFAAEVCKSYDNYIGKDFASQMNEENYIDTD"
     gene            7054..8772
                     /locus_tag="PKZ74_05845"
     CDS             7054..8772
                     /locus_tag="PKZ74_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92314.1"
                     /translation="MKKITLIQIRAGLYGFFVPFKDLYKSIPSNSLTFILFLFIPVFY
                     IAASSIAYNARAPYYLFSIDPEYFYMYNGVILGAGNLSIQYYAHPGTPLHFLVAISTR
                     ITDLFQQGSYMENFVNDPEMFIRSANLFINVIIAIILFIAARFTLKYTGSVWAALTVQ
                     LSPFASFSLPEMAGRVYPEALMMAPLLLIIVMVIKNTFEPEKQHNLQKDLILYALIIG
                     FGAAVKLTFVPLLLLPLLLLKTSLREKMRYFAYSVLFFLIFAYPLLFNIGDSWDWITG
                     IMVHSGKYGTGERNFIDISAAGMNARNLLEMNSGFFYLVTASLLIIMVLSLNPVKKRL
                     FPDQRIIRAILAIDLVVIACMAPILKHYSLYYFLPFTIFKFPVFLLLTALLLRIKVVA
                     ETRPFRLIIVLFACVYFVFTLAVQVGQFRTHAAINTAKHAKTIENARKLVSVIDPGKP
                     IIIASPYYGSPFIEFAHYNGYIMSRHMKGFYTDYLREKFPGCYMSPTWSENFYSWDNF
                     VDFKDILATVKSSFYIYIGCGMEKDLPVIKSRISQVLDMNSVMARTLYDDPDTGEQLI
                     EIIVNQ"
     gene            8775..10523
                     /locus_tag="PKZ74_05850"
     CDS             8775..10523
                     /locus_tag="PKZ74_05850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM92315.1"
                     /translation="MKIFYRNFLILFLLLFVSVDGFSQAIYPRNYFRSPLEMRLLLSG
                     TFGELRSNHFHSGIDIKTAGTEGAPVYAIADGYVSRIKVSAYGFGKVLYVTHPNGFVS
                     VYAHLSKFSRIIGDHVKGEQYRRELFEIELFPEESRFPVKKGDIIAYSGNSGSSGGPH
                     LHFEIRDAAQKTINPLLFGFEVKDFFKPRILSVKIYPENENSRVNGKNKPVRFLTEGW
                     GTEHRITGNPAITVSGDISFAIQAYDQQNDTDNKNGAYAVRLFIDSAKVFDFDVETFA
                     FDETRYINSYLDYEEYVKNDVRLQRTKIDPGNKLSMYNQLNNNGVYHFSDTLTHSIRY
                     EVADVAGNTAVLAFKVKSVKPLLNKAETDTTRTPGFDFPFSLFHWDRPNHFNNGSIVL
                     DAPKGVFYDSFEFSVDSAAMIPGTYTRVYRIHDKYTPVHDYMTLSIRPSDVPEELLAK
                     ALIVKVGDDGKSFSSAGGEWESGYIRTKIREFGNYTIAVDTVPPKVRAVNPGQFANMA
                     GQKTLKLTVSDDLSGIASYRAVLNGKWILLEYDPKNNLMIYTIDEHMVPGKNNLLVEV
                     RDGKNNRFYYSAVINL"
     gene            10565..>11234
                     /locus_tag="PKZ74_05855"
     CDS             10565..>11234
                     /locus_tag="PKZ74_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM92316.1"
                     /translation="MEGNIRDIQLNKLNERAKELKCIYQVCEALRDEKADLIKVFKSI
                     LDSIPPGYQYPTVCEARINFEGKQYSSTDFKETEWIQRAEIVIDNHISGEIQVCYIQD
                     VFEPNRRIFLPEEQKLLNTIADHLSRYIFQRRLKKTLELSLFPENGADYNGESSLLNY
                     ESDEHWKWRLQVAEKIAGMMDMERFGVEAVYLIGSTKSGQAGPGSDIDLLVHFRGKPG
                     QEKEL"
BASE COUNT         2950 a         2655 c         2758 g         2871 t
ORIGIN      
        1 agtgaagggt gaacagtgaa gggtgaacag tgaagggtga acagtgaagg gtgaagggtg
       61 aaaatggaaa ataatggaag gttggatgat cattaagaat taaaaattaa gaattaaaaa
      121 ttaagaatta gaattttaga tttcatgaaa aagactgtat tttgtggttt gcacgaggca
      181 aacggtggga aaatggttga attcgcaggc ttctatatgc ctgtccagtt tgaaggagtc
      241 aacgctgagc atgagacggt gcgtaaggga gtcggggtgt tcgacgtttc gcacatgggc
      301 gagttctgga tcaaagggcc aaaagcattt gagctggtcc agcgcctgac cagcaacgac
      361 gtggctaaac tggacgacgg aaagatccag tacacctgtt ttccgaatga taagagcggg
      421 atcgtggacg acctgctgac ctaccgtttt gatgccgaaa cctatctgct ggtagtgaat
      481 gcggctaata ttgataaaga ctgggcctgg gtggtgaaga acaacattca cggcgccacg
      541 atcttcaatg catccgacga gatttcgcaa ctggcgatcc aggggcctaa agcgcaggcc
      601 gttatgcaaa aactcaccga taccgatctt aataccattc cttattatac tttcaaaaag
      661 gtcgatctgg ccggagtcaa acaggttatc ctgtcgaata caggctacac cggtgcgggc
      721 ggtttcgaag tctatatggc caatagcgac ggccctgcca tctggaaagc cctgatggag
      781 gccggagcgg agtttgatat caaaccgatc ggcctggcgg cacgagatac gttgcgcctg
      841 gaaatgggat tctgccttta cgggaatgat atcaacgata ccacctcccc gctggaagcc
      901 ggcctgggat ggatcaccaa atttgccgat ggaaacgatt ttataaaccg acctgccctg
      961 gaaaaacaaa aatcggaagg tttaaagcga atgctccgtg gttttgagat gatcgataaa
     1021 ggaattccgc gtcagcatta tgaaatttgc gatgaaaacg gccgggtgat cggagaggtg
     1081 acctccggca caatgtcgcc ttcgctgaaa atcgcgatcg gaatgggtta tgtcgaaaca
     1141 gcccattccg ggatcggaaa ccacgtcttt atcaggatcc gtgaaaaatt gcttaaggca
     1201 atcatcgtaa aaattccctt ttacaagggc taacgtcgct taaaaatgat gaaggggcct
     1261 gtcgcagacg tctgtttttc acccgccttg ttaccgggat ttgaaaaacg gaccgatcgt
     1321 attgttgtgg tcattgatat ccttcgtgcc acaacggcga tgtgcacggc ttttggcttt
     1381 ggcgttaaat cggtgatccc ggtgatgaag aaagaaactg ccctggccag gaaagagaaa
     1441 ggctggctgg tggcgggaga ggagaacggc ataaggctgc catttgccga tttcggcaat
     1501 tcaccgcgtg agttccggtc aaaagccatg aaaggaaaag agatcgtttt ctgcactacc
     1561 aacggaacaa aggccatact gaaagggagg agctacgggc aggtaatcat agcatcgttt
     1621 gtaaatcttg aggcggtgtg tacttatatc aaaaagcaga agcgtgatgt actgatattg
     1681 tgtgcaggat ggaaagaccg tttttccctg gaagattcgg tggcagcagg cgccctggcc
     1741 gaccggctga tccatgcata cggctatttg ccaggaaacg atgccgcact cgccgcaatt
     1801 tcgttatgga accaggcctc aaaaagcctg ctgaagaccg tttcccgttc cgaccattac
     1861 ctgaggctcc tgggaattga agaccggaaa ggcctgcgtt attgtttttt cccggagaaa
     1921 ttccgtgtcg tacccggttt gcaaactaat aagcttatta acctgccgga aatcaaagaa
     1981 taaagacaat gatcgcaata aggatggctg ttccggatgc gaccagcaca ccgccggcag
     2041 cggcatcttt ggcccatttg atcttttcat tgtagtcggg gctgatcagg tcgcccagcc
     2101 attcgacggc tgtattgaag atctcggcga tcagcaccag cccgatggcc agcaccacaa
     2161 gtccccattc catcagcgtg attttctgat aatagccgat gacaaagacc acgccgatag
     2221 ccagcaggtg gatgatgaag ttgggctggg tgatggccac gccggagaag ccccggatgg
     2281 cgtatttaaa gcttttcagc aggcggatga ggaacttttt taacaggttg cgcatggtct
     2341 gaatttttcg ttaaaattag aaattttgcc cgggtacagg atatttatgg atgcgaaaaa
     2401 tattattttt gcatcaaatt gcggaagtag cccagttggt agtcccgagc actcgggatc
     2461 ccaagctgag ggtcgataga ataagcggaa gtagctcagt tggtagtccc gagcactcgg
     2521 gatcccaagc tgagggtcga tagaataagc ggaagtagct cagttggtag agcatcagct
     2581 tcccaagctg agggtcgcgg gttcgaatcc cgttttccgc tccgaaggcc ggcgaaagcc
     2641 ggcttttttc ttccaacgtt ttttatcata tttgcaccga aatttatcca aaaattatta
     2701 atgatgaaaa atatattgat gctttttgta tttatcctta ccggatctgt gacgttcgcc
     2761 cagaaaagca acctggtgtt ctataccgag cagggcgaga cattttacct gaatatcaac
     2821 ggaataccgc aaaattacga accggtttcc aacctgatga tctccgacct ggtggatcag
     2881 acctgtaaag taaccctgaa gtttgaggat ccggcattgg gtgagatcag taagaacctc
     2941 acattcggcc agggctcgga gaccacctac gtgatccgga tgaatgccag tgatgaatgg
     3001 atcctccgct atgttaaaga ggtgccggtc gcccaggctc ctgcgccgcc ggccgaccgg
     3061 tcagtgatcg ttctgcgaac aacagcaccg gcaaaaccgg tgaagaccgg acagagtcag
     3121 tctaccgcta accaggcgac aacgacctca acgacctcgg aagttgccac cgaaggcggt
     3181 tcggtgggga tgaatgtcca ggaaccgggc ggcaatatca cgatcaattt caacgtcagc
     3241 ggggtaggga catccaccag caccagcact accacttcgg ctacaacttc aaccgtaacg
     3301 ggaaatacgg acgtgcagaa tcctgagccg gagccagtgg ccgtgatcga aaaaccgaaa
     3361 ccggtggcca ttccgggtta cagcggcctg atcggctgcg actggccaat gtcggagtac
     3421 gatttcagca tggcaaaaaa ctccatcgaa tccaaatcgt ttgaagacag caagctgacc
     3481 cttgccaaac aggtgttcga ttccaaatgc atgctctgca tccaggtcaa agagatcatg
     3541 aaacttttca gttttgaaga gtcacggctc gaatttgcca aatatgccta tggctacacg
     3601 tacgacctga gcaatttcta catcgtgaac gatgccttct ccttcgaatc gtcgatcgat
     3661 gaactgaaca aatacatccg gtcgcagaat aactgaggca acgatttgcc ttcggattgt
     3721 atctttcttg atcagtgcaa ccttgtccct ttccggtctg aattgcatgc attttaacta
     3781 cttttgccct gatgaataac cgcagggtaa tcagcataat aaaaatcctg atctggatcg
     3841 cccccctctt gccgtggtct ttgctccctg gtcaggaact catgcctgtc ctgacgcatg
     3901 aaatgacccc ggaagaagag ctgcgcaaag gggaaatcgg gcggagcttt acgccgacca
     3961 gcccacccct ggccccggcc cgggctatcg ccgaattcga gcagatgcaa agcgtgctca
     4021 tccgctatcc gttcggaatc ccgatgtcgc tgatcgtcga aatggccgac gactgcatgg
     4081 tgacgaccat cgtagccagc caggcacaac ttgaccaggt gctgaaccag tatatcgccg
     4141 ccggcgtcga tacatcgcac tgcgaatggc tcatcgcgcc aaccaattcg tattggaccc
     4201 gcgactacgg cccgtggttc gtagccgaca gctcacacca ggtgggcatt tgcgacttcc
     4261 cctacaaccg tccccgccca tacgacgata atatccccgt ggtgctggcc tcccagatgg
     4321 atatccctct ctatggcatg aacctcatcc ataccggcgg caactggatg tgcgaagggc
     4381 tgggaaacgc ctcgtccacc gagctggtct ggaccgaaaa tcctacgctc acacaccagg
     4441 aaatcgacga cctggtggag agtttcctgg gcattgatac ctaccatgtt ctgcccgatc
     4501 cgctgggaga atacatccag catatcgatt gctgggggaa gttcctggat gttgataaag
     4561 tactgatcgg gcaggtcgcc gaatcggatt accggtacga tgattacgaa tatgtggccg
     4621 gctattttgc ttcgcagatc agcgcttatg gtacgcctta ccaggtcttc cgggtctata
     4681 cgccgggcga ttatccatat acgccgtaca ccaattcatt gatactgaac aaaaaggttc
     4741 tcgttccggt aaccggcagc ccgtgggatg atgaggcgct cggcgcctat gcggatgcga
     4801 tgccgggtta cgaggtgatc ggcgtcattt attcgggatg gcaaaatacc gatgcccttc
     4861 attgccgggc aatcgggata gccgataccg gcatgctgta tattcaccat atgcccctct
     4921 ccggagagca ggaattcagc ataggttata cgatcagttg cagtatcatc ccatatacgg
     4981 atgaggaatt gatttccgat tcgctgttgt gtatttataa aatcggtaac ggttctttcc
     5041 agacggtacc gcttgccagt ggcggtggtt ccgtcttttc ggcttcactc ccttatattg
     5101 cgcccggaag ccaggtctcc tattaccttc atgccgccga ctcttcgggc cgttcgatga
     5161 gccatccctt catcggatct cctgatccgc atgtttttac ggttcagaat gaggtccccg
     5221 gcctgtggac cggcgaagtg tcgggcgact ggatgaatcc ctccaactgg agcaataatg
     5281 ccgttcccgc atctccgact caagtcgtta tccctcccgg atcatcccac tggccggtat
     5341 tcagcggtga cctggtggtc ggctctacct gcggcaatat catcatcacc ggaacggcac
     5401 aaatgacggt aacgggcagt gttaccttac atcccggaaa aaccatcacc atccttaaca
     5461 acggccacct gatccaaacc caaacaccat aatcccgtaa cccttgagcc ctgccatact
     5521 ggtactccta cggagttcac tggattttaa actttggaac cctggaacat atgaactcag
     5581 ggaccctgga acattggaac atttaaaaca tttccctata tttgcctctt aaaacccacc
     5641 ctatgaaaaa caatcttttt aaactacttt ttacgactat tgcgattttt gctaatgtat
     5701 cgctgtttgc ccaggctcct gccgatgcca ttaccggaaa atggctgaat gaagacaagg
     5761 atgcccatat cgaagtttat aaggagagcg gcaaatattt tggtaaaatt gtctggctga
     5821 aagacccgat cgatgaggtg acggggaaac cgaagctgga tgacgaaaac gacgatgaag
     5881 cgctgcagag ccgcccgatc ctggggctgg tgatcctgaa ggattttatc tacgacggcg
     5941 atggagaatg ggaagacgga accatttatg accctaaaaa cggtagtacg tatgattgtt
     6001 atattcagtt tggtgatgat aagacgctga aaatcagagg ttatataggg atttcctgga
     6061 tcggacggaa tacttactgg acgagggttg aatgaggtca atagattttc gattttcgat
     6121 ttaggataaa atgtggcatg aatgcaatat acgccatcgg tgaaaccgtc tacgatatta
     6181 ttttccgcaa tgatcagccg gttgcagccc gaccgggcgg atcgatgctc aatacagcca
     6241 tttcactggg acggagcgga ttgaaggtgg aaatgattac cgaactgggc gatgacccgg
     6301 tgggtaaact ggttactgct tttttgaacg ataacggggt ttcaacatcc ttcatccagc
     6361 ctgcaggtaa ttataaaacc cccgtttcac ttgcgtttct tgatgattcg ggcgatgccc
     6421 gttattcctt ctataaaaat attccagaga aaaggctgaa catcggtcag gctgaggccg
     6481 gtaagggtga tgtggtcctt ttcggatcgt tttattcgct cgacgccgga atcagacatg
     6541 aaatcatttc attcattaat aaatcgaaac aaaacggtgc tttgatcttc tatgacccga
     6601 atatccgcaa aaatcacctc ggggagatca ataaactcat gccttccgtg gaagaaaata
     6661 tttcccttgc cgatataatc cgcggctcgg atgaggattt ttttaacctt ttcggccttg
     6721 acgatcaatt tgaaatagct cataaagtga ttcaatcagg ctgtcatcac atggtcatta
     6781 cccgcgggaa acatggggcc gactatcttt cgcgggatga agcgattcac tttcctgcga
     6841 tagaaaccaa agcggtcagt acgatcggcg caggcgatgc gtttaatgcc ggaatcattt
     6901 atgcaattat taaaaatgac attcccggaa tagaacttgt taatatcaat tcagcgacct
     6961 ggaaaacaat tctgagaatt ggaactattt ttgccgcaga agtttgtaaa agttatgata
     7021 attacatcgg gaaagatttt gcaagccaaa tgaatgaaga aaattacatt gatacagatt
     7081 agagcaggcc tgtacggctt cttcgttccg tttaaggatc tttataaatc cattccatca
     7141 aattctttaa cttttattct attcctcttt atccctgtat tttacattgc agcttcctcc
     7201 attgcttaca atgcccgggc cccctattac cttttcagta tcgatcctga atacttctac
     7261 atgtacaacg gagtgatcct tggagccggt aacctgagta tccagtatta tgcccatccc
     7321 ggtacccctt tgcattttct tgtcgccatt tcaacacgga tcaccgacct ttttcagcaa
     7381 ggaagctaca tggagaattt tgtcaatgat ccggaaatgt tcatccgttc tgcaaatttg
     7441 tttatcaatg ttataattgc gatcattctc ttcattgccg ccaggtttac actgaaatat
     7501 accgggtctg tttgggctgc gctgactgtt caactgagtc catttgcctc tttttcgctg
     7561 ccggaaatgg ccggaagggt atacccggaa gccctgatga tggctcccct gctgcttatc
     7621 atcgtcatgg tcatcaaaaa cacctttgaa ccggaaaaac aacacaatct gcagaaagac
     7681 cttatacttt atgctctgat catcggattc ggggctgcgg tgaaactgac gttcgtccca
     7741 ctgctacttc ttcccctgtt gttactaaag acatcacttc gtgaaaaaat gcgatatttt
     7801 gcttattcgg tgttgttctt tctcattttc gcctacccgc tcttatttaa tatcggcgat
     7861 tcatgggact ggattacagg aatcatggtt catagtggaa aatatggcac cggggaaaga
     7921 aatttcattg atatctcagc tgcaggaatg aatgcaagga atctgttaga gatgaacagc
     7981 ggattctttt acttggtgac agcttcactg ctgattatca tggttttgtc attaaatcct
     8041 gtcaaaaaaa gattatttcc cgaccagagg atcatccggg ccatactggc cattgatctc
     8101 gtggttattg catgtatggc ccccatcctt aaacattatt ccttatatta ctttttacct
     8161 ttcaccattt ttaaattccc ggtatttctg ctgctgaccg cattactctt aaggataaaa
     8221 gtggtagcgg aaacaagacc tttccgattg atcattgttc tgtttgcttg cgtttatttt
     8281 gtttttacat tggcggtaca ggtgggacag ttcaggacgc atgcggcgat caacactgca
     8341 aaacatgcaa aaaccattga aaacgccagg aagctggtct ccgtcatcga tcccggcaaa
     8401 ccgatcatca tcgcatctcc ttattatggc tcccctttta ttgagtttgc tcattacaat
     8461 ggttacatta tgagccgtca tatgaaagga ttttacacgg attacctgag ggaaaagttc
     8521 cccggctgct atatgagtcc gacctggtcg gaaaattttt attcatggga caattttgtg
     8581 gacttcaaag acattttagc tacggtgaag tcatcattct atatttatat cggctgtgga
     8641 atggaaaagg atcttccggt gatcaagagc aggatcagcc aggtactgga catgaattca
     8701 gtgatggcca ggacgcttta tgatgatccg gataccggtg agcagcttat cgaaatcatt
     8761 gttaatcaat agttatgaag atattttata gaaatttcct gatattattt ctcctgttat
     8821 tcgtttctgt agacggtttc tcacaggcaa tttatcccag gaattacttt cgttcaccgc
     8881 tggaaatgag gctgttgctt tcaggaactt tcggagagct gagatccaat cactttcatt
     8941 cgggtatcga tattaaaaca gccgggacgg aaggggcccc ggtttatgcc attgccgacg
     9001 gctacgtgtc gcggatcaag gtttcggctt atggctttgg aaaagtattg tacgtgaccc
     9061 atcccaacgg gtttgtttcg gtatatgctc acctcagcaa attcagccgt atcattggcg
     9121 accatgtcaa aggcgaacag taccggcgcg aactatttga gatagagctg tttccggaag
     9181 aaagccgctt ccctgttaag aaaggggata tcattgctta ttccggcaat tcagggagtt
     9241 cgggcgggcc gcacctccat ttcgagatcc gtgacgctgc gcaaaaaacc attaatcctt
     9301 tgctctttgg ttttgaagta aaagactttt ttaaaccccg catcctctct gtcaagattt
     9361 accctgaaaa tgagaattcc cgggttaacg ggaaaaacaa gcctgtccgc tttcttaccg
     9421 aagggtgggg aacggaacac cggatcaccg gcaatcctgc gatcactgtc agcggtgata
     9481 tatcctttgc cattcaggct tacgaccagc agaacgatac tgacaacaaa aacggcgctt
     9541 atgccgtccg ccttttcatc gattctgcca aagtcttcga ttttgatgta gagacctttg
     9601 cttttgatga aacacgttat atcaacagtt atcttgacta tgaagaatat gtgaaaaatg
     9661 atgtcaggct gcaacggact aaaatcgatc cggggaataa gctgagcatg tataaccagt
     9721 tgaataacaa tggcgtatat cattttagcg atacactgac gcattcaatc cgttatgagg
     9781 tggccgacgt tgcgggcaac accgccgtgc tggctttcaa agtgaaatcg gtcaaaccct
     9841 tgctaaataa agctgaaacg gatacaacaa gaacacccgg tttcgatttt cccttttccc
     9901 ttttccactg ggaccggcct aatcatttca acaatggctc cattgttctc gatgccccga
     9961 agggtgtttt ttacgactcc tttgaatttt ctgtggattc tgccgcgatg ataccgggaa
    10021 catacacccg ggtatacagg atccatgata aatacacccc tgttcatgat tatatgacgc
    10081 tttcgatccg tccgtcggat gtccctgaag agctgctggc caaagccctt atcgtaaaag
    10141 tcggcgatga tggtaagagc ttttcatcgg ccggcgggga atgggaaagc ggctatatcc
    10201 gcaccaaaat ccgcgaattc ggcaactata cgatcgcggt cgatacggta ccaccaaaag
    10261 tcagggcagt gaatccgggc cagtttgcca acatggcggg ccagaaaacc ctgaaactga
    10321 cggtcagcga tgatctctcc ggaattgcct cctaccgcgc cgtgctcaac ggaaagtgga
    10381 tcctgctgga gtacgatcct aaaaacaacc tgatgatcta tacgattgac gaacatatgg
    10441 ttcccgggaa aaacaacctg ctcgttgagg tccgggatgg aaaaaacaac cggttctatt
    10501 actcggctgt aataaatttg taaattcgtg cctgccggtt tttaagcctg tcaatattat
    10561 cgagatggaa ggaaatatca gggatatcca gttaaacaag ctcaatgaga gggcgaaaga
    10621 gctgaaatgc atctaccagg tctgcgaggc gttgcgtgat gaaaaagccg acctgatcaa
    10681 ggtctttaaa tccatcctgg attcgatccc gccgggctac cagtatccga cggtgtgcga
    10741 agcccgtatc aactttgaag gcaagcagta ttcctcaacc gattttaaag aaacggagtg
    10801 gatccagcgg gctgaaatcg tgatagataa ccatatttcc ggggagatcc aggtgtgtta
    10861 tatccaggat gttttcgaac cgaaccgccg gatttttctg ccggaagaac aaaagttgct
    10921 caataccatt gccgaccatc tcagccggta tattttccag cgcagattga aaaaaacgct
    10981 cgagctgtcg ttgtttcctg aaaacggagc ggattacaac ggcgaaagtt cccttcttaa
    11041 ctatgagtcg gatgaacact ggaaatggcg gttgcaggtt gccgaaaaga ttgcaggcat
    11101 gatggatatg gaaaggtttg gggtcgaagc cgtttatctg atcggcagca cgaaaagcgg
    11161 acaggccggg ccgggaagcg atatcgacct gctcgttcat tttcgtggaa aaccgggcca
    11221 ggaaaaggaa ttgc
//
LOCUS       DAOPNH010000082        11198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6198_length_11198_cov_6.590595, whole genome shotgun sequence.
ACCESSION   DAOPNH010000082 DAOPNH010000000
VERSION     DAOPNH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_11198_cov_6.590595"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1736
                     /locus_tag="PKZ74_05860"
     CDS             195..1736
                     /locus_tag="PKZ74_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPM92317.1"
                     /translation="MIRRRTIIYLLIDILIVTVSFLLFIWIKPASRAVYLPTYFPPFV
                     FFLFLWLSVSVSIDKYRLYKKKTLRDVLFPVIIGDLIILFAVVLLIVLFQQFNYSRMI
                     VFGTIGLSFLLELLFSYIYYYNRNLSRDAEHFDTFAQARIKAKAGLLEPEEVEQLLRE
                     RPDAPSLPLNRELIRQEAGEEVLEYIESFIDAEHRRTLVISTTTPFNIQSQPEGYFDA
                     IVNLHQVNDFKRINKFFEAVNSRLPMYGLYINCAYTNAIRKRKIMRKYPPVINGIFYF
                     FHFIFMRIFPKLPLTKNIYFWITNGYNRSLSKAETFGRLYSCGFELINDRQIGNLHYF
                     VARKIQEPSFDYHPTYGMLIRLRRVGKGGKIIYVYKMRTMHPYSEYLQDYVYKTSDLQ
                     EGGKFRNDFRVSTTGRIMRKLWIDELPMLINLLRGDLKLVGVRPLSQHYFELYSEELR
                     AKRIRTRPGLIPPFYADLPKTLEEIQESENRYLDAYLRNPFFTDWRYFWKAFYNIVVR
                     RARSN"
     gene            1867..3546
                     /locus_tag="PKZ74_05865"
     CDS             1867..3546
                     /locus_tag="PKZ74_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92318.1"
                     /translation="MSKKIFITLMLIAGSLLSQSQVVYEHISRRSIYDFLDELANAKI
                     IEVNSAIKPYSRTFIAEKLKTAAGNTGQLSKRQQQELEFYLKDYRLELEDNTTGMKPL
                     NLFPKNDHVATALNPLGFHYSDPVFTASVRPVWGIGYYSNSNGGNYHRWGGMEAFAYI
                     GENVGGYTSLRDNYEDIQMTSPAYFNQRPGSPTKDSPKGGLEYSEARGGLMASWKWGA
                     IGIVKDHNEWGNNYYGSNILSGRTPSFAQVKLQLTPVRWFDFNYFHGWLVSEVVDSSR
                     SYWDGDRYRVVFYPKYIAANMFTFRPVRHLAVSVGNSIIYSDMNVHIAYLVPFLFFKS
                     VDHTLNATNSAGYTGQNSQLFVDISSRNIKNLHLYLSAFVDEFSIKRVTDPGRFNFLS
                     WKGGFRLSNWPVKNVFLSSEYTYTSPATYQHNISTTTFESNLYNLGHYLRANSQDLWV
                     EVACKPLRGLTVSATFNRAVHFNDYEYRTIAYIDTVPVMQDLSWRKTEIGLTGRYEFL
                     NNAYVFAGITFSNIEGFEADGNIPDFYLDKYTPEFFHGKTTTLRAGFNIGF"
     gene            3707..5452
                     /locus_tag="PKZ74_05870"
     CDS             3707..5452
                     /locus_tag="PKZ74_05870"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPM92319.1"
                     /translation="MKRLTIALAFIFMLSAAYKSFACTNFLVTKGASKDGSVMITYSA
                     DSHTLYGELYHWPAAKWPAGTMLDVYEWDTGKYLGKIPQAAETYNVTGNMNEHQVSIG
                     ETTYGGREELATQAGAIMDYGSLIYIGLQRARTAREALVVITELMAEHGYASSGESFS
                     VADKNEAWIFELIGKGEGQKGAVWVARRIPDGYVCAHANHARITTFPLADGKTSITYK
                     DRARIMNPEVECFYADDIITFAREKGWYKGKDKDFNFSDVYAPVDFGGARFCEVRVWS
                     FFKSVNKDLKGYMDYASGHIKYDETLPDGTVNENKFPTNRMPLWVKPDHLIDIHELMD
                     YMRDHLEGTELDMTKDLGAGPWGSPYRWRPLTWTIDSVTYCNERATATQQTGFWFLAQ
                     SRSWLPDQIGGIFWFGVDDAASSVLMPMYASMKRAPEKMAAGYGAMMEFVDDAAFWVF
                     NQISNLAYTRYSYIHPEIREKQQETEKNFIAMIAEVDEKAKSIYEANPAKAVDFLTDF
                     SVNAGNNTVADWKKFYGYLFTRFMDGNIKTAVPVPDGYIYHAPKVEQPGYPEWYLRLI
                     VETTGDKLKVIGGGH"
     gene            5633..6799
                     /locus_tag="PKZ74_05875"
     CDS             5633..6799
                     /locus_tag="PKZ74_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPM92320.1"
                     /translation="MHYHFDTIIERAGTSSVKYDLRKALFGRDDVIPMWVADMDFAVP
                     HFVEEAVLNRARHPIYGYTAIPESYTEAVAGWQQSRHNWEIDNNWLFFSPGVVAGLNM
                     IVQAFTRPGDAVIVQPPVYFPFFSCVENNGRRLVLNQLKEQNGDYTIDFEDLEQKMKA
                     GARMMILCSPHNPVSRSWSREELERLGEKCLEYGILMVSDEIHGDLVFGPHRHIPMAS
                     ISPEIADNTITCIAPSKTFNLAGLFTSSIIIPNEKLRETFKKHQETLHLSPNLFGLVA
                     AEAAYREGGSWLDELMQYLKDNISIVSGYLSRNLPDITFSKAEATYMLWLDFRKTGIP
                     PAEMKSRLVEKAGLGLVEGPVFGQGGNGFQRMNIACPRTVLIGALERLPLALNP"
     gene            6840..7766
                     /locus_tag="PKZ74_05880"
     CDS             6840..7766
                     /locus_tag="PKZ74_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92321.1"
                     /translation="METNPNTNISGGRSNRTLITILVISAIVVIGLIIILLQTRSNLK
                     GLLTEKEQQRQELTRELDSLLVEHNKVKTEYGRLSDSLVSKDSVIQANAEEIKRLLNT
                     EWEYYKVRKKLAQLQTVAQGYVLQMDSLYRVNASLTEENTAIKKDLKDLRKEKEQIES
                     DRKVLTGKVEMAAVLKAFNMDAVGLRLRSGGEKEVTTDKAQRIDQVRICFNIAENKIA
                     TPGSKEIYIRIARPDQEILTKSQIDDYSFEYQGERLQYSVMETIEYENEEQEVCVYWK
                     KEYSSQEMQAGLYHVDIFCENAVIGHTTFTLR"
     gene            complement(7771..8370)
                     /locus_tag="PKZ74_05885"
     CDS             complement(7771..8370)
                     /locus_tag="PKZ74_05885"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HPM92322.1"
                     /translation="MKKLYEGLNKSIKKYKLYVLKDVIIFIIITLAVHFTYRFWVNIL
                     DYWPVNPLMHRAHDFMSSVVYNQSSVIIGNILNIPFTEGENRFFYFENTGFIAINESC
                     SGLKPMLQFLLLMIIFPGPWKHKAWFIPMGLVIVHLTNLFRISGLAVVTITLPEYWDF
                     AHDNIFRPFFYVVIFLLWVWWAEKFRPRFAKRIKTENSG"
     gene            8446..>9771
                     /locus_tag="PKZ74_05890"
     CDS             8446..>9771
                     /locus_tag="PKZ74_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92323.1"
                     /translation="MSNHGHHHRSHRRRSALGRFIDRLTGRRRRSSFHRSEFPGIHLP
                     GEKEEGTRKYRILEERSAPDTSPEITGTGANETAGQSASTGSSSRRRRKESFFKLYLK
                     KRALRKEEQYKEKMRRAHRRRSEKEYRKLNQGPGLGKKLLDLARDEEEAAKKIPLLSN
                     RSRFFRNMTIVVNSMMIFMITYVLTYLFYWLSCMLVASWYGLDSILYFYDLRFNDHSP
                     LWSRFNILLITGIPPFLCLVAGAFLYKAVFKIKRFAGLQKLFILWSAFHLLNHFFGAF
                     PSGVVTDEGLGYVAAWMYMNTAVKFLFSLLFLFGLGLIGFYSAKPILETSDSLGRIKA
                     ERKTSFMFYQMGLPWLIGTFVMLLLRIPKNFDYPYETLMFFTMAFLVVPPFFNEKVKP
                     ELNLLKTKKKRQINIGYLAMVLVLLAFLRIMLGIGLHFIIEISISISPAS"
     assembly_gap    9772..9871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9873..10493)
                     /locus_tag="PKZ74_05895"
     CDS             complement(9873..10493)
                     /locus_tag="PKZ74_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HPM92324.1"
                     /translation="MAEKKFYVVWKGKKAGIYDTWKECEAQVKGVEGARYKSFPGKQE
                     AERAFSAGWVKPAGKRVSNPVSKAGVSNPVWDSIAVDAACSGNPGVMEYRGVITRSGK
                     QIFHKGPFPLGTNNIGEFLGIVHGLAYLKKEGKDIPVYTDSRTAIKWVKDRAVKTKLP
                     RNTKTEPLFELVDRALAWLNANEYPNTILKWETAIWGEIPADFGRK"
     gene            10556..11029
                     /locus_tag="PKZ74_05900"
     CDS             10556..11029
                     /locus_tag="PKZ74_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPM92325.1"
                     /translation="MNKYTLLLLLCIALLTSCGPRKIYEKHYDIPRITWSRFDVRTFD
                     VEIKDISASYDFYIAIRHHTEIPFKNIETKLTIYTPSGEMRMNEQKIVLRDNEGKLLG
                     SGMGDLWDIKYLARKGLKFTEKGTCRVEISSTMSKADLPGIIQVGLIVEKGNSKR"
BASE COUNT         2857 a         2810 c         2763 g         2668 t
ORIGIN      
        1 gtgaatccgt tggttgccag tgatcgtgct ccaacgacta aagccgttgg ttccaaaaac
       61 aatagaaccg acggctttag tcgtcggact acccactttc tcactgacgg ctccagcata
      121 tatacctgat ccaaactccg tgtaatctgt ggtgaaaaaa aataactaat tttaaccgcc
      181 taaccccaat cccgatgatc cgacgccgga cgattattta cctgctgatc gatatcctga
      241 tcgtcaccgt ttcattcctg ctgttcatct ggatcaaacc ggcatccagg gcggtctatc
      301 tccccaccta cttcccccct ttcgtgttct tccttttcct gtggctcagt gtttccgtct
      361 ccatcgacaa ataccgcctt tacaaaaaga agaccctgcg cgatgtcctg tttcccgtca
      421 tcatcggcga cctgatcatc cttttcgccg ttgtcctgct gatcgttctc ttccagcagt
      481 tcaattattc caggatgatc gtcttcggaa ccattgggtt gtcgtttctg ctggagctac
      541 tgttttccta catctactat tacaaccgga acctgagccg cgatgccgag catttcgaca
      601 ccttcgcaca ggcccggatc aaagccaaag ccggcctgct ggaacccgaa gaggtggaac
      661 agcttctccg cgagcggccc gatgcccctt cccttccgct gaacagggag ctgatccggc
      721 aggaagcggg cgaggaggtc ctggaatata tcgaatcatt tatcgatgcg gaacaccgcc
      781 gcacgctggt gatatccacc accacgccgt tcaacattca gagccagcct gaggggtact
      841 tcgatgctat cgtgaacctg caccaggtaa acgatttcaa gaggatcaac aagttctttg
      901 aggccgtgaa cagccggttg ccgatgtatg gcctttacat caactgcgcc tataccaatg
      961 cgatcaggaa acggaagatc atgcggaaat acccgccggt gatcaatggg atcttttatt
     1021 tcttccattt tattttcatg aggatcttcc ccaaactgcc gttgaccaag aatatctatt
     1081 tctggataac gaatggctac aaccggtcgc tttcgaaggc agagactttc ggacggttgt
     1141 attcctgcgg gttcgagctg atcaacgacc ggcagatcgg caatctgcac tatttcgtgg
     1201 cccggaagat acaggagccg tcattcgact accatccgac ctacgggatg ctgatccgcc
     1261 tccgccgggt gggaaaaggg ggaaagatca tctatgttta taaaatgcgg accatgcatc
     1321 cctattccga atatttgcag gattatgtgt ataagacgtc ggacctgcag gagggaggga
     1381 aattccggaa cgatttccgt gtgagcacta cgggcaggat catgcggaag ctctggatcg
     1441 acgagctgcc gatgctgatc aacctgctgc ggggcgacct gaagctggtc ggggtgaggc
     1501 cgctgagcca gcattatttc gagctgtaca gcgaagaact gcgggcgaaa cggatccgta
     1561 cccgcccggg actgatcccg ccattctatg ccgatctgcc gaaaacactg gaggagatcc
     1621 aggaatcgga gaaccgttac ctggacgctt atttgcgcaa tcctttcttc accgactgga
     1681 ggtatttctg gaaggcgttt tataatatcg tggtacggcg ggcgaggagt aattgagttt
     1741 tcatgttgat tattaaccac ataggcacat agaacacata gagaacacat agatttgtat
     1801 tcatgaaaat tcatcctatg tgattcctat gtgccctatg tgtctatgtg gtttaaatag
     1861 aatcctatga gtaaaaaaat cttcattact ttaatgttga ttgcgggatc actcctgtca
     1921 caaagccagg tcgtctacga gcacatttcc cgacgatcga tttacgattt cctggacgag
     1981 ctggcgaatg caaagatcat cgaggtcaat tcggccatta agccctacag ccggacgttc
     2041 atcgcggaga agctgaaaac cgctgccgga aataccggtc agctcagcaa gcggcagcag
     2101 caggagctgg aattttacct gaaagactac cgcctggagc tggaagataa cactaccggc
     2161 atgaagccgc tgaacctttt cccgaaaaac gaccatgtgg ctaccgcttt gaatcccctg
     2221 gggttccatt actccgaccc ggtttttacc gcatcggtac gcccggtgtg gggcatcggt
     2281 tattattcca attctaatgg cggcaattac caccgctggg gcgggatgga ggcatttgcg
     2341 tatatcggag agaatgtcgg cggatacacc agcctgaggg ataattatga agacattcag
     2401 atgacctccc cggcctattt taatcaaagg ccggggtctc cgacaaaaga cagtcccaaa
     2461 ggcggccttg aatacagcga agcgcgcggc ggcctgatgg cctcctggaa atggggcgca
     2521 attggaatcg taaaagacca taacgaatgg gggaacaact attacggctc caacatcctt
     2581 tcgggccgga ccccttcctt tgcccaggtt aaactgcagc tcacgccggt ccgctggttc
     2641 gatttcaact attttcacgg gtggctcgtg tcggaggtgg tcgacagcag ccgttcctac
     2701 tgggacggcg accggtaccg cgtggtcttc tacccgaaat atatcgctgc caacatgttc
     2761 actttcaggc cggtaaggca cctggccgtc tctgtgggca actccatcat ctacagcgat
     2821 atgaatgtgc atatcgccta cctggttccc ttcctgttct tcaaatcggt cgaccatacg
     2881 ctcaatgcca ccaacagcgc cggttataca ggccagaact cgcagctttt tgtggatatc
     2941 agctcccgca acatcaaaaa cctccacctg tacctcagcg cgttcgtgga tgagttttcc
     3001 atcaaacggg tgaccgatcc cggccggttc aatttcctgt cgtggaaagg cggcttcagg
     3061 ctgagcaact ggccggtaaa gaatgtattt ctctcatcgg aatacacgta tacatcgccg
     3121 gcaacatacc agcataacat ctccaccact accttcgaat ccaatctata caacctgggg
     3181 cattacctgc gcgccaattc gcaggatctg tgggtggaag tggcctgcaa gcctttgcgc
     3241 ggcctcacgg tgagtgcaac cttcaaccgg gccgtccatt tcaatgatta cgagtaccgg
     3301 acgatcgctt atatcgatac ggttcccgtg atgcaggact tatcctggcg gaaaacggaa
     3361 atcgggctca ccggaaggta tgagttcctg aataatgcat atgttttcgc cgggatcaca
     3421 ttctccaaca tcgaaggatt tgaagcggac gggaatattc ccgatttcta ccttgacaaa
     3481 tacacgcccg aatttttcca tggaaagacg acgactttac gtgccgggtt taatattgga
     3541 ttctaaattt attaattttg tcactgattc gtgttaatta gttgaattcg tgttaatttg
     3601 tgttccataa aagaacgcga atgacgcaaa tcctgacgaa tgaccacgaa ttattttaat
     3661 tttgcgcaat tcgacttatt aaaaactact aattcaataa attataatga aaagactaac
     3721 cattgcttta gcctttattt ttatgctctc tgcagcttat aagagcttcg cctgcaccaa
     3781 tttcctcgtc acgaaaggcg cgtcgaaaga cggatcggtg atgatcacct attcggctga
     3841 ttcacatacc ctttacgggg agttatacca ttggccggca gcgaaatggc ctgccgggac
     3901 catgctggat gtttacgaat gggacaccgg caaatacctg ggcaagattc cgcaggctgc
     3961 tgaaacatat aatgtcactg gcaatatgaa cgagcaccag gtttcgatcg gagagaccac
     4021 ctatggcgga cgggaagaat tagcgacaca ggccggcgcc atcatggatt acggcagcct
     4081 gatatacatc ggcctgcagc gtgcccggac agcgcgggaa gcgcttgttg tcatcaccga
     4141 actgatggcc gagcacggct atgccagctc cggcgaatcg ttttccgtag ccgataagaa
     4201 cgaagcctgg atatttgaac tgatcggcaa aggcgaaggg cagaaagggg ccgtttgggt
     4261 agcccgccgc atccccgacg gatatgtctg cgcccatgcc aaccatgccc ggatcaccac
     4321 attcccgctg gccgacggca aaacgtcgat cacctataaa gaccgcgcca ggatcatgaa
     4381 cccggaagtg gaatgcttct atgccgacga catcattaca tttgcccgcg aaaagggctg
     4441 gtacaaaggc aaagacaagg atttcaattt ttctgacgta tatgccccgg tcgatttcgg
     4501 cggcgcccgt ttctgtgaag tccgtgtctg gtcgttcttc aaatcggtga acaaagacct
     4561 gaagggctat atggactatg ccagcggcca tatcaaatac gacgaaacct tgcccgacgg
     4621 cacggtcaac gaaaacaaat tcccgaccaa ccgtatgccg ctctgggtaa aacccgacca
     4681 cctgatcgac attcatgagc taatggacta tatgcgcgac cacctggagg gaactgaact
     4741 cgacatgacg aaagacctgg gggcaggccc ctggggcagc ccgtaccgct ggcgtccgct
     4801 cacctggacc atcgacagcg tgacctactg caacgaaagg gctacggcca cccagcaaac
     4861 cggtttctgg ttcctggccc agtcgcgttc ctggcttccc gaccaaatcg ggggcatctt
     4921 ctggttcggt gtggatgatg ccgccagcag tgtcctgatg ccgatgtatg cctcgatgaa
     4981 acgcgccccg gaaaagatgg cggccggcta cggcgctatg atggaatttg tggacgatgc
     5041 cgcattctgg gtcttcaacc agatctccaa cctggcctac acccgctatt cctatatcca
     5101 tcctgaaatc agagagaaac agcaggaaac cgaaaagaac ttcatcgcca tgatagccga
     5161 agtagatgag aaggccaaat cgatttacga agccaacccg gcaaaagccg ttgattttct
     5221 gaccgatttc agcgtgaatg ccggcaacaa tacggttgca gactggaaaa agttctatgg
     5281 ctacctgttc acccgcttca tggacggcaa tatcaagacc gcagtccctg tgcccgacgg
     5341 ctatatttac catgcgccaa aggtcgagca gcccggttac ccggaatggt acctgaggct
     5401 tatcgttgaa accaccggcg acaagctgaa ggtgatcgga ggcggacatt aggattggac
     5461 gatttggaga tttggagatt tggagatttg gggatttggg gattggacgg tatgatttta
     5521 taaattatta atcggtgttg atccgtcgaa tccgtgtaaa ttcgtgttct attcggagca
     5581 attgaattta cacggatttt ttcatttaaa gtctttttga catcacatta aaatgcatta
     5641 ccattttgat acaatcatcg aacgtgctgg aacaagttcc gtgaaatacg acttgcgaaa
     5701 agcccttttc ggcagagacg atgtgatacc gatgtgggtg gctgacatgg attttgccgt
     5761 tccgcacttt gttgaggaag cggttttaaa ccgcgccagg caccccattt atggttatac
     5821 ggccattcct gaaagctaca cggaggcggt ggccggctgg caacagagcc gccataactg
     5881 ggaaatcgat aacaactggc tgtttttcag ccccggtgtt gtcgccggcc tgaatatgat
     5941 cgtgcaggcc tttacccggc ccggtgatgc cgtgatcgtt cagcccccgg tttattttcc
     6001 cttcttttcg tgtgttgaaa acaatggcag gaggctcgtt ttaaatcagc ttaaagaaca
     6061 aaacggcgac tataccatcg actttgaaga tctggaacag aagatgaagg ccggggccag
     6121 gatgatgatc ctgtgcagcc cgcataaccc ggtaagccgg agctggagca gggaagaact
     6181 ggaacggctg ggtgaaaaat gccttgaata cgggattctg atggttagtg acgaaattca
     6241 tggcgacctg gtattcgggc ctcaccgtca tattcccatg gcttccatct cgcctgagat
     6301 agcggataac accatcacct gcattgctcc gagcaagacc ttcaatctgg ccggattatt
     6361 cacctcttcc atcatcatcc cgaatgaaaa actaagggaa acatttaaaa aacaccagga
     6421 gacccttcac ctcagcccga acctgttcgg gcttgttgcg gcggaagcag cttaccggga
     6481 agggggttcg tggctggatg agctgatgca atatctgaaa gataatatca gcatcgtttc
     6541 cggttacctt tcccggaatc tccctgacat caccttcagt aaggcagaag ccacctatat
     6601 gctctggctc gatttccgca aaaccggcat accgccggct gaaatgaaat cccgcctggt
     6661 ggagaaggcg gggctgggac ttgtagaagg accggtattc ggacagggcg ggaatggatt
     6721 tcagcgtatg aatattgcct gtccgcgcac tgttctgatc ggggcattag aaagattacc
     6781 cctggcactc aatccctgac ggcaatttta ttcttatttt tgtgttaaat aattgattta
     6841 tggaaaccaa tccaaataca aacatttccg gtggccggtc aaatcgtact ttaataacga
     6901 tcctcgtgat ttctgcgatc gtggtcattg ggctgataat cattttatta caaaccagaa
     6961 gcaacctgaa aggcctgctg acagaaaaag agcagcagcg gcaggaactt acgcgggaac
     7021 tggattcact cctggtcgaa cacaataagg tcaaaacgga atacgggcgc ctttccgatt
     7081 cgcttgtttc caaagacagc gtgatccagg ccaatgctga ggagataaaa cggttgctga
     7141 acacggaatg ggaatactac aaagtcagga aaaagctggc ccaactgcag actgtggcac
     7201 agggatatgt cctgcagatg gattcgcttt accgggtcaa cgccagcctg acagaggaaa
     7261 acacagcgat caagaaagat ttgaaagatt tacgcaagga aaaggagcag attgaaagcg
     7321 accgcaaagt gctgacgggc aaggtggaaa tggccgccgt cctgaaagct ttcaatatgg
     7381 acgccgtcgg cctgagactg cgcagcggcg gggagaaaga ggtgaccacc gacaaagccc
     7441 agcgaataga ccaggtaagg atctgtttca acattgcgga aaataaaatc gccactcccg
     7501 gctcaaaaga gatctatatc cgcattgccc gtcccgacca ggagatcctg actaaaagtc
     7561 aaattgacga ttattctttt gagtaccagg gcgaaagatt gcagtactct gtcatggaga
     7621 ccattgaata cgaaaatgag gagcaggagg tctgcgttta ctggaagaaa gaatactcaa
     7681 gccaggagat gcaggcgggt ctttaccatg tcgacatttt ctgtgaaaat gccgttatcg
     7741 gtcatacaac atttactctg agataaagga ttaaccagaa ttttcggttt taatccgctt
     7801 cgcgaatctg ggtctgaatt tctccgccca ccagacccag agcaggaaaa tcactacata
     7861 aaagaatggc ctgaaaatat tgtcgtgggc aaaatcccag tactctggta aagtaatggt
     7921 cacaacggcg aggccgctta tcctgaacag gttggtgagg tggacaatga caagccccat
     7981 cgggataaac caggctttat gcttccaggg cccgggaaag ataatcatca gcagcaaaaa
     8041 ctgcagcatc ggtttcagtc ccgaacagct ttcgtttatg gcaatgaaac cggtgttctc
     8101 aaagtagaag aaccggttct ccccttctgt gaaagggata ttcaggatat tgccgataat
     8161 caccgagctc tggttataaa ccacacttga catgaaatca tgagcccggt gcatcagcgg
     8221 attgaccggc cagtaatcga gaatattgac ccagaagcgg taagtaaagt gaacggcaag
     8281 ggtgatgatg ataaagatta tcacatcctt caatacatat agcttatatt tttttatact
     8341 tttgttaagc ccttcgtata actttttcat cctttgccag tatcatgagg aagtatgcgg
     8401 ctaaggtaag attttttaca gcctttgttt attaatccgg agaaaatgtc caatcacggc
     8461 caccatcaca gaagtcaccg caggcgcagc gccctgggca ggtttattga caggctgaca
     8521 ggccggcggc ggcgcagttc atttcaccgt tcggaattcc cgggaatcca tcttcccggc
     8581 gaaaaagaag agggaaccag gaaataccgg atcctggaag agagaagtgc tccggataca
     8641 tcacctgaaa ttaccggaac cggcgccaat gaaacagccg ggcagtcagc gtcaaccgga
     8701 tcgtcttccc gccgaaggcg taaggaaagc ttctttaagc tctacctgaa aaaaagggcg
     8761 ctgagaaaag aagagcagta caaagagaaa atgagaaggg cccaccggcg gagatcagag
     8821 aaagaatacc ggaaactgaa ccagggcccc ggattaggca aaaaactgct tgacctggcc
     8881 agggacgaag aagaggcagc aaaaaagatc cccctgctgt cgaaccggag ccgctttttc
     8941 aggaacatga ccatcgtggt gaattcgatg atgatcttca tgatcaccta cgtactgacc
     9001 tacctgttct actggctgag ctgtatgctg gtggcttcct ggtacgggct ggattccatc
     9061 ctctatttct acgatctgag attcaatgat cactcgccgt tgtggagccg gttcaatatc
     9121 ctgctcataa cgggtattcc cccgtttctg tgcctggtgg caggcgcttt tctttacaaa
     9181 gccgttttta agatcaaaag atttgccgga ttacagaaac tttttatcct ctggtcggct
     9241 ttccatctcc tgaaccattt cttcggcgct ttcccgtcgg gtgtcgtaac cgacgagggg
     9301 ctgggctatg tagccgcctg gatgtacatg aacaccgctg tgaagttcct cttctccctg
     9361 ctgtttttat tcggattggg gctgatcggt ttttattccg ctaaaccgat cctggaaaca
     9421 tccgacagcc tgggccggat caaagcggag agaaaaacat cgttcatgtt ctaccagatg
     9481 ggattgccct ggctgatcgg caccttcgta atgcttttgc tcagaatccc caaaaacttc
     9541 gattacccgt atgaaaccct gatgttcttt accatggcgt tcctggtggt tccccctttc
     9601 ttcaacgaaa aggtcaaacc ggaactcaac ctgctgaaaa ccaagaaaaa acggcagata
     9661 aacatcggtt acctggccat ggtactggta ctgctggctt tcctgaggat catgctgggc
     9721 atcgggctgc attttatcat cgaaatttcc atttcgatct caccggcatc gnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattatttgc gtccgaaatc ggccggtatt
     9901 tccccccata ttgccgtttc ccatttcagg atcgtattcg ggtattcgtt tgcattcagc
     9961 caggccagtg cccggtcaac gagctcgaac aacggttccg ttttggtgtt gcgggggagt
    10021 tttgtcttga cggcccggtc ttttacccat ttgatggctg tccgggaatc ggtgtaaaca
    10081 gggatgtctt tcccttcctt tttgagatag gccagtccat ggacaattcc caggaactcc
    10141 ccgatgttgt ttgtcccgag cggaaatgga cccttgtgga aaatttgttt ccccgaccgg
    10201 gtaattacgc cgcggtattc catcaccccc gggttcccgc tgcaggcggc atcaacggcg
    10261 atgctgtccc agacagggtt ggaaacccct gctttggata cagggttgga aacccttttt
    10321 ccggccggct ttacccagcc cgctgaaaat gccctttctg cctcttgctt tcccggaaac
    10381 gacttgtacc gggccccctc aacacccttt acctgggctt cgcactcttt ccacgtgtcg
    10441 tatattcccg ccttttttcc cttccagacc acataaaatt ttttttcagc catccccgat
    10501 tgtttaacag gctaaaataa tcatcatttc cgttattttt gttaaaagga taatgatgaa
    10561 taaatatacg ctgttattat tgctttgcat agccctcctc acctcctgcg gcccccggaa
    10621 aatctatgaa aagcattacg atatccccag gatcacctgg agccgtttcg atgtcaggac
    10681 tttcgatgtg gagattaagg atatcagcgc ttcctatgat ttctatatcg ccatccgtca
    10741 tcataccgaa atcccgttta aaaacattga aacaaaactg acgatctaca ccccctccgg
    10801 ggagatgcgg atgaacgagc aaaagatcgt cctccgcgat aatgaaggta aactcctggg
    10861 aagcggtatg ggcgacctgt gggatatcaa gtacctggca aggaaagggc tcaaattcac
    10921 cgagaaggga acctgccgcg tcgaaatatc cagcaccatg tcgaaagccg atctgcccgg
    10981 catcatccag gtggggctga tcgtggagaa agggaatagt aaacggtgaa cggtgaacgg
    11041 tgaacggtga acagtgaacg gtgaacagta aatagtgaac ggtgaacggt gaacggtgaa
    11101 cggtgaacag tgaacagtga acagtgaacg gtgaacagtg aatagtgaac agtgaatagt
    11161 gaattaggaa tactgatgta aaacctgaaa cctgaaac
//
LOCUS       DAOPNH010000083        11179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6214_length_11179_cov_7.322456, whole genome shotgun sequence.
ACCESSION   DAOPNH010000083 DAOPNH010000000
VERSION     DAOPNH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6214_length_11179_cov_7.322456"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /locus_tag="PKZ74_05905"
     CDS             complement(<1..2366)
                     /locus_tag="PKZ74_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM92326.1"
                     /translation="MKRLLFILLTASVVACSPKRTPIPETGFIPGIAMPVRLDKGQPE
                     IILADLAPGVSIIDSVSLYGRILKLSGDKQVAAINVGTDAPPLMELKIWSGGQALSVP
                     VMKSRKADILYSFDPGNSVYEKVELAGEFNGWTPSRTPLKFENGNWVTTLSLNPGRYQ
                     YQLVTDGKWRIDPANPDSVDNNNGGFNSVLKAGPSDRSAQPQLFASGYDLKSNEIGIT
                     WTSKPDEFLVFWQNIRLDETYLEQEENELNIFIPEEAKRFGRSFIRVFAYNGYGLSND
                     LLIPLQNGKVVLDPADLTRADRQSMIIYNVFTDRFFNGNPSNDKRVGNPQVVLPKADY
                     FGGDIAGVTQKIREGYFNDLGVNTLWISPVVLNPEGAYGQWNDPKTKFSAYHGYWPVS
                     FTLIDPRMGTPEEFREMVSEAHARGINVLLDFVAHHVHELHPYYIEHPSWTTSLYLPD
                     GSLNTEKWDEHRLTTWFDVFLPTLDLQQPEVTEMLSDSAVWWIKHYNLDGFRHDATKH
                     VPEIFWRALTRKLKQQVVMEEGREVYQIGETYGGPELIAGYIGSGMLDGQFDFNVYDA
                     AIGVFARESDPFTNLDKTLQTSLDFYGYNNQMGYITGNQDRGRFISYAGGDLKFGENA
                     KAAGWTREIGVGDTIGYRRLSMLTAFNMTIPGLPVIYYGDETGIPGGNDPDSRRQMKF
                     SDLTPLEMRTRNLATDLTRIRKDNLALVYGSFSTVEVSDKIYVFVRKYFDNEVIVIFN
                     KNNRLERIEADLPHWMDCKNMKAMFGHDFKINERKLSVEIQPWSFEIIV"
     gene            2483..3976
                     /locus_tag="PKZ74_05910"
     CDS             2483..3976
                     /locus_tag="PKZ74_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM92327.1"
                     /translation="MKKKKNNIRAIPENPVIYEINTWVWLRELGEKYRKKINLGNVPA
                     KEYDNLAATGIDTVWLMGVWKRSPAGLEIAKNNEGIMNDLRNALPDLTDDDISGSPYC
                     IMDYQADKKIGGEKGLAKARKELADRGIRLILDFVPNHVAPDHPWTVKNPGFFINGSE
                     QDLAEHPEAFLKAGNHIMARARDPYYPPWPDVLQLNVFNRGLRDELVKTLDHVASQCD
                     GIRCDMAMLVMNDIFSKTWGDKAGPAPGTDFWKHVIPAIRKKHPEFVFIAEVYWDMEA
                     ALFNQGFDYCYDKRYYDDLKEGPEKARQHLEGMTLYEGKLLRFLENHDEPRAASLFHP
                     AKNMALALASLTLPGARLFHDGQLEGRSVKVPVFLSRRQKEPINEPLNRFYLLLLKIL
                     KYHEVRYGTWAPCELSGWHDNQTCQNMLAWEWTGEHENLIIVVNLSDDPSQAHLKSGY
                     PWLPGKTYQLFDVTSGELYRRDGEEMNDPGLYIILQGWGMHALSIGH"
     gene            complement(4015..5865)
                     /locus_tag="PKZ74_05915"
     CDS             complement(4015..5865)
                     /locus_tag="PKZ74_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPM92328.1"
                     /translation="MKATLIRILSAIILAIPLVGFGQAEIKRIDPPFWWTGMAENRLQ
                     LMVYGEGLGQVQPALSYPGVKMTGYSALENPYYLVIDLEISENARPGKVPISFQYQRE
                     LLFVLDYELRARTSGSAERKGFGKEDVIYLIMPDRFSNGDPANDTISGMFEPASHANP
                     DGRHGGDLKGITDLLPYLADLGVTALWLNPVLENNQPAYSYHGYAITDFYRVDPRFGS
                     NEDYLEFVGKAHQEGLKVIMDMVFNHCGSNHLWIQDLPSHDWIHQFPEYTRSNFRAET
                     VMDPYASEFDRTKMMTGWFDKPMPDLNQHNEYLANYLIQNSIWWIEYAGLDGIRMDTY
                     PYSYKDFMTRWMERINKEYPGFSVVGETWLQKEAHTAYWQSGSAAGAGAVTHLDHVTD
                     FPLQYAIKDAFTGEEGWTSGLARIYYVLSQDYLYADPFGNVIFVDNHDLTRFYSSIGE
                     NFAKFRMAMAILLTTRGIPMIYYGTEYLMTGEEPKGHGYIRENFPGGKWQSAVGSWQS
                     VAGGEELGIEFLKGLLSWRKTSEVVRNGKLMQFVPDDGIYVYFRYNATESVMVIINKN
                     TTDKALKLYRYQERLEKYSRGKDIITGQEYLLGEDLSIPPETVLILELSH"
     gene            6224..7936
                     /locus_tag="PKZ74_05920"
     CDS             6224..7936
                     /locus_tag="PKZ74_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM92329.1"
                     /translation="MKDRFSDLYRIWNILYPEHPTDKLDALIGEILSFSGKTAIPPLE
                     EEWYKNAVVYSLYVDLFSSDFKGLTGRLEYLQDLGVNCLWLLPILDSPMRDAGFDISH
                     YRKIRKDLSGLGEKATDDDLDRIFRDFLDEAHRRGMKVIFDIATNHVSDQHPWFREAI
                     KSKDNPYRDYFIWSPTGEEYAGARIIFYGIEESNWKKEGDEYYFHRFFDFQPDLNYRN
                     PEVLAEMSRNLLFWLERGVDGFRADAIPYIWKEEGTPCENHPNTHNIVKFFRAVIDHA
                     RPNTLLLAEACQKPWEVVKYFGDGDECNAGYHFPLMPQIFKAVAMGSSQPVMHTLSTA
                     VTPEIPGSAQWFTFLRCHDELSLELVYVTEEDRKYIHDHYCRKPQWDFRRGQGISARL
                     SELMERNPKKIGMIFSIMLTLPGTPVIYYGDEFGKFNDEEYYNEMKISVGKDDTRFLV
                     RGKIDWKRVENALADDSTFESAVFSQIRQMLHARNSLAVFGRGSMEWVHLRSGDGNPA
                     ASVMAYIRSLDGKRVLVIHNLSGNTVPVKWEFPGGKTILGPDPVRNGDLMEISGFGYH
                     WLTL"
     gene            7965..11120
                     /locus_tag="PKZ74_05925"
     CDS             7965..11120
                     /locus_tag="PKZ74_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HPM92330.1"
                     /translation="MDMQSFDAVIFDLDGVITKTALVHSAAWKKMFDGYLREREIKYG
                     EPFREFSQKEDYLPYVDGKPRYKGVESFLESRGIKLPFGTPEDDPEKETVCGIGNRKN
                     IAFNEALKSDGVQVYESTVALIRRLKEEGIRVGVASSSKNCRQVLETAGLLDMFETRV
                     DGEVSAELGLKGKPEPDIFTVAADNLGVTYDRAVVVEDAVSGVQAGRKGNFGLVLGLA
                     REENENELYSNGADIVVKDIADIGLEGIVDWFRQGLFKDNWSLTYHDYIPEREKTREA
                     LLTVGNGYFGTRGAMPETIAGPYNYPGTYMAGIYNRLVSKVGDKEIENEDFVNVINWL
                     PVTFKINDEEWFDVNNARIAEVRRTLHFNNGMLYRRLVVRDDAGRETLIGTRRIASMD
                     NPNLAAQEYTVTPLNYSAKITIRSGLYGDHINAGVERYSQLNQKHIEPVLLGSEENIQ
                     YMVVKTTRSQNVIAAAAQVTVFEGNDRVYVHFHKNHTAGRSRIEFTKKLAQGEALRIQ
                     KIVALCHNRDQDETDPLDSAIENCKKFRTFDEVLERSARKWEEIWDRIDIRVAGDRLA
                     QKLLRLHLYHTLITTSPLNRNIDAGIPARGLHGEAYRGHIFWDELFILPFYNLHFRDV
                     VKSVLLYRHKRLDEARKYALEYGYQGAMFPWQSGSSGREETQILHLNPLSGEWGPDHS
                     SLQRHVSLAIAYNTWQYYHITGDLAFMEEYGAELIFEICRFWAGKSKLNRVTGRYEII
                     KVMGPDEFHEKYADTDEGGLKDNAYTNMMVAWLFGRAAVLFDRMSPQAKETVMAKIHL
                     ATGEIERWNDIAARLNLVISPEGIIAQYDGYFDLLELDFEEYRKKYKNIYRMDRLLKA
                     EGKSPDEYKVAKQADTLMTFYNLSGEEVGEILEKLSYKLPDDYLMRNLNYYLARTSHG
                     STLSRVVHAQLANMTGNKSLAWELYQDALTSDYADIQGGTTAEGIHAGVMTGTVWVAL
                     VSFAGLNLHGDYPRFNPQLPAHWRKISFFFSFRDGEYECEVTAAGIRIRVDKPEPGSA
                     RIGLQDKTFEINTGEWVDLEI"
BASE COUNT         2915 a         2811 c         2806 g         2647 t
ORIGIN      
        1 acgatgatct caaatgacca gggttgaatc tctacagata gtttccgttc atttattttg
       61 aaatcatgac cgaacatcgc tttcatattt ttacaatcca tccagtgagg aagatcggcc
      121 tcaattcttt ccaaccggtt gtttttattg aagatgacga tcacttcatt atcaaaatat
      181 ttacgaacaa aaacataaat cttatccgaa acctccactg tcgaaaaact gccgtagacc
      241 agcgccaggt tatctttccg gatacgggtc aggtcggtgg ccaggtttct tgtcctcatc
      301 tccagcgggg tcaggtcgct gaatttcatc tgccggcgcg agtcgggatc attaccgccg
      361 ggtatgccgg tttcatcgcc gtaatagatg accggaagcc ccggaatggt catattgaag
      421 gctgtcagca tcgacaagcg gcggtacccg atcgtgtcgc ccaccccgat ctctcgggtc
      481 cagccggccg ctttggcatt ctcgccgaat ttcaggtcgc cgccggcata agaaataaag
      541 cggccccggt cctggttccc ggtgatatag cccatctggt tgttatagcc gtaaaaatcc
      601 aggctggtct gaagggtctt gtcaagattg gtgaaaggat cggattcacg ggcaaaaacg
      661 ccgatggccg catcgtaaac attaaaatcg aactgtccgt cgagcatgcc cgaaccgata
      721 tagccggcga tcagctccgg gccgccgtag gtctcaccga tctgatagac ctcccggcct
      781 tcttccatca ccacctgctg cttcagcttg cgggtaagcg cccgccagaa aatctccgga
      841 acgtgcttcg tggcatcgtg gcggaatcca tccaggttgt agtgcttaat ccaccagact
      901 gccgaatcgg acagcatctc cgtaacttca ggctgctgca ggtcgagcgt cgggaggaaa
      961 acgtcgaacc aggtcgtcag ccggtgctcg tcccactttt cggtgttgag cgatccgtcg
     1021 ggaaggtaaa ggctggttgt ccacgatgga tgttcgatat aataaggatg cagttcatgg
     1081 acatgatgcg cgacaaagtc gagcaatacg ttgattcccc tggcatgcgc ttcgctgacc
     1141 atctccctga attcctccgg cgttcccatc cgggggtcga tcagggtgaa cgaaacgggc
     1201 cagtagccat ggtaagcgga gaactttgtc ttcggatcgt tccactggcc gtaggcgcct
     1261 tccgggttaa ggacaaccgg ggagatccac agcgtgttca cacccaggtc attaaaatat
     1321 ccttccctta tcttctgagt cacacctgcg atatcaccgc cgaaatagtc ggctttgggc
     1381 aaaaccacct gagggtttcc aacccttttg tcattcgacg ggtttccgtt aaagaaccgg
     1441 tctgtaaaaa cattgtaaat gatcatcgac tggcggtcgg cccgcgtgag atcagcagga
     1501 tccagcacga cttttccgtt ttgaagcgga ataagcaagt cgtttgataa accgtatccg
     1561 ttgtaagcaa aaacacggat gaacgaacgg ccaaacctct tcgcttcttc cggaataaaa
     1621 atattaagtt cgttttcttc ctgttcaaga tatgtctcat ccaggcgaat attttgccag
     1681 aaaacaagga attcatccgg ttttgatgtc catgtgatgc caatttcatt gcttttcaag
     1741 tcatacccgg aagcaaagag ctgcggctgt gccgaacggt cggacggtcc cgctttcaaa
     1801 accgaattaa aaccgccatt gttgttatca accgaatcgg gattagccgg gtcgatcctc
     1861 catttaccgt cggtcacaag ctgatactgg tatcgtccgg gattcagcga aagtgtcgtc
     1921 acccaatttc cattttcaaa tttcaaaggc gtcctcgatg gtgtccagcc attaaattct
     1981 cctgccagtt ccactttttc ataaacacta tttcccgggt caaatgaata aagaatatcc
     2041 gctttcctgc ttttcatcac aggcaccgac agtgcctgcc cgccgctcca gatcttcagt
     2101 tccatcagcg gtggcgcatc tgtcccgaca ttgatcgctg caacttgttt gtcgccggaa
     2161 agtttcagta tcctgccata gaggcttacc gaatcgatga tagacacgcc aggggccagg
     2221 tcggccagga tgatctccgg ctgccctttg tcgagccgca caggcatggc aatacccgga
     2281 atgaagccgg tttccggaat aggtgtacgc ttcgggctgc aggcaacaac cgaagccgtc
     2341 agcagaataa acagtaatct tttcatggga aaagcatttg ggattgcaaa tatcgtaaag
     2401 aaaaactaaa aaaaattata cttttatagg gtcttacttg cgttactaat gaagaaatga
     2461 agtttccatt aatcccaaca caatgaaaaa gaaaaagaat aatataaggg ccatccccga
     2521 aaaccctgtt atttatgaga ttaacacatg ggtctggctc cgggaactgg gggaaaaata
     2581 caggaaaaag attaacctgg gcaatgtacc ggcaaaagaa tacgacaacc tggcggcgac
     2641 gggaattgat acggtctggc tgatgggtgt ctggaaaaga agcccggcag ggctggaaat
     2701 tgcaaaaaac aatgaaggga tcatgaatga tctccggaat gctcttcccg acctcacgga
     2761 tgatgacatt tccggctcac cttattgcat catggattac caggcggata agaagatcgg
     2821 cggggaaaaa gggcttgcca aagcgaggaa agagctggcc gaccgaggca taaggcttat
     2881 cctcgacttt gttccgaacc atgtggcccc cgaccatccc tggacggtca aaaatccggg
     2941 ttttttcatc aatggcagcg aacaggacct ggcagaacat ccggaagcat ttctgaaggc
     3001 aggaaatcac atcatggcaa gggcccgcga cccctattat cctccttggc ctgatgttct
     3061 gcaattgaat gtatttaacc ggggcctgcg tgacgaactc gttaaaacac tcgatcacgt
     3121 tgcttctcag tgcgacggca tccggtgcga catggccatg ctggtcatga atgacatatt
     3181 ttcgaaaaca tggggagata aagcaggccc ggccccggga accgactttt ggaaacatgt
     3241 tataccggct atccggaaaa aacatcccga atttgttttt attgcagagg tatactggga
     3301 tatggaagcg gcgcttttta accagggatt tgattattgt tacgacaaga gatattacga
     3361 tgacctgaaa gagggccctg aaaaagcccg gcaacacctg gaagggatga ccctttacga
     3421 aggtaaactg ctccggttcc tggaaaatca cgacgaaccc agggctgcct ctctgttcca
     3481 cccggcaaaa aatatggccc tggcactggc ttccctgacc cttcccgggg ccaggctttt
     3541 ccacgacggc cagctggagg gaagaagcgt taaggttccc gttttcctgt ccaggaggca
     3601 gaaggagcca ataaatgaac cccttaacag attttacctc ttgctcctga aaatcctgaa
     3661 gtaccacgag gtcagatacg gaacctgggc gccctgtgaa ctttccggat ggcacgataa
     3721 ccagacgtgc cagaatatgc tggcatggga atggacaggg gaacatgaaa accttatcat
     3781 tgttgttaac ctcagcgatg atccatccca ggcacacctg aaatcgggct atccctggct
     3841 cccgggtaaa acttaccaac tgttcgatgt tacttccggt gaattatacc gccgcgacgg
     3901 ggaagagatg aacgatcccg gcctctatat cattttgcag ggatggggaa tgcacgcact
     3961 gagcatcgga cattgacatg ttccgttttg cattgtacgt ttttcatttt tcatttaatg
     4021 ggatagttcc aggattaaaa ccgtttccgg cggaatggaa agatcttcgc ccaatagata
     4081 ttcctgaccg gtaatgatat ccttaccccg ggaatatttc tccagcctct cctgatacct
     4141 gtataatttc agtgctttat ccgttgtgtt tttattgatg atgaccataa cggattctgt
     4201 tgcattatac cggaaataga catagatgcc gtcgtccggc acaaactgca tcagctttcc
     4261 gttgcgcacc acctccgagg tcttcctcca actcagcaat ccttttaaaa actcaatccc
     4321 taattcctca cctccggcta ccgactgcca actgccgacc gccgactgcc acttccctcc
     4381 cgggaaattc tcccggatat acccgtgacc cttcggctct tcacccgtca tcaggtattc
     4441 cgttccgtaa taaatcatcg ggatgccacg cgttgtcagt aaaatagcca tagccatcct
     4501 gaatttggcg aaattttcgc cgatgctcga ataaaaccgc gtaaggtcat ggttatcaac
     4561 gaaaataaca ttaccgaacg gatccgcgta aaggtaatcc tgcgaaagca catagtaaat
     4621 ccgggcaagc ccgctggtcc acccttcctc cccggtgaat gcgtctttta tagcatactg
     4681 cagcggaaaa tcggtgacat gatccaggtg ggtaacggct ccggcgccag cagctgaacc
     4741 cgattgccag taagccgtat gggcctcttt ctgaagccag gtctcgccaa ctacgctgaa
     4801 tccgggatat tccttgttaa tcctctccat ccagcgtgtc atgaaatctt tgtaagaata
     4861 gggataagta tccatcctga tcccatcaag accggcatat tcaatccacc atatgctgtt
     4921 ctgtatcaga taattggcca gatactcatt gtgctggttg aggtcaggca tcggcttgtc
     4981 gaaccaacct gtcatcattt tcgtccggtc gaattcggaa gcatacgggt ccataacggt
     5041 ctccgccctg aaattggatc gggtgtattc cggaaattga tggatccagt catgcgaagg
     5101 caaatcctga atccagagat ggttgcttcc gcaatgattg aacaccatgt ccatgatgac
     5161 tttcagtccc tcctgatgag cttttccgac aaattcaaga taatcttcat tcgacccgaa
     5221 ccttgggtcg acccggtaaa aatcggtaat ggcatatccg tggtaagaat aggcaggctg
     5281 gttattttca agcacggggt tcagccagag ggcagttaca cccaggtcgg ccaggtaggg
     5341 aagcagatcg gtaatccctt tgagatcgcc cccgtgccgc ccgtcgggat tggcatgact
     5401 ggccggttcg aacatcccgc ttatcgtgtc gttggccggg tcgccattgg aaaagcgatc
     5461 gggcatgatc aggtagatca cgtcttcctt tccaaaaccc ttgcgctcag ccgatcctga
     5521 cgtccgggcc ctcagttcat aatccagcac aaacaacagc tcacgctggt actgaaaact
     5581 gattggcact ttaccgggtc gtgcattttc cgagatctcc agatcgataa ccaggtaata
     5641 ggggttctca agcgcggaat aacctgtcat tttcacaccc ggatagctga gagccggttg
     5701 cacctggccc aacccttcgc cataaaccat aagctgaagc ctgttctctg ccatgccggt
     5761 ccaccagaac ggcggatcga tcctttttat ctccgcctgg ccaaatccca ccaaaggaat
     5821 tgccaatata attgccgata atattctgat aagggttgcc ttcataagga tttttatctt
     5881 agcggccgcg aagttacgga aaattaaccg gatcattttt caacagtgac ctattcataa
     5941 gtaatcgtta aatcctgaaa atatcccgga ttattatagt aaacttacat tcggaatttg
     6001 aaccggccgg atcgttccaa gacgggttgc tgttagagtc agccaataat agccgagaaa
     6061 cttccctgtc tgaaaaaagc cctgaacgtt agtccggggc ttttttgttt ttgaggcttg
     6121 cattttaaaa tcacaatgga taaatttata cttttgccgg acaattaatc cggtttatat
     6181 ttttctggat ttatttggtt atcagcaatt ttaccgattg tgcatgaaag atagattttc
     6241 tgatctttac cggatctgga atatattata cccggaacat ccgacggaca aactggatgc
     6301 cttgattggc gagatcttgt ccttttcagg aaaaacagcc atccctccac tggaagaaga
     6361 atggtataaa aacgctgtcg tatattcgct ttacgtcgat ctgttcagca gcgatttcaa
     6421 gggtttgaca ggtcggctgg aatacctgca ggatttgggg gttaactgcc tgtggctact
     6481 gccaattctc gattcgccca tgcgcgacgc aggatttgat atcagccact accggaaaat
     6541 caggaaagat ctgtcgggcc tgggtgaaaa agcgaccgat gacgaccttg accggatttt
     6601 cagggatttc ctggatgagg cgcaccgaag aggaatgaaa gttatcttcg acatcgcgac
     6661 aaaccatgtc tccgaccagc acccctggtt cagggaagcg attaaatcca aagataatcc
     6721 ttaccgtgat tactttatct ggagcccgac cggtgaagaa tatgccggcg cccgtatcat
     6781 tttttacggt atcgaggaga gtaactggaa aaaagaggga gatgaatatt attttcaccg
     6841 gtttttcgac ttccagcccg acctgaacta ccgtaatccg gaggtgctgg cggagatgtc
     6901 aagaaacctg ctgttctggc tggaacgtgg cgtcgacggt ttccgggccg atgcgatccc
     6961 gtacatctgg aaagaagagg gaaccccctg cgagaaccat cctaatacgc acaacattgt
     7021 gaaattcttc cgggccgtga tcgatcatgc ccgccccaat acgcttcttt tagccgaagc
     7081 atgccagaaa ccctgggagg tcgtgaaata cttcggcgat ggggatgaat gcaatgccgg
     7141 ttatcatttc ccgcttatgc cgcagatctt taaggcagtg gctatgggca gcagccagcc
     7201 ggtcatgcac acgctgagta cggccgtcac acccgaaatt cccggaagcg cccaatggtt
     7261 tactttcctg cgctgccacg atgaactgag cctggaactg gtctatgtga ccgaagaaga
     7321 ccggaaatat atccatgatc actattgccg aaaaccacaa tgggattttc gtcgcggaca
     7381 gggcatatca gcccggcttt cggaattgat ggaacgaaat cccaaaaaaa tcgggatgat
     7441 cttttccatt atgctgacat tgcccggaac acccgtgatc tactatggcg atgagttcgg
     7501 aaagttcaat gacgaagaat actacaacga gatgaaaata agtgtcggga aagacgacac
     7561 acggttcctg gtcaggggta agatcgactg gaaaagggtg gaaaatgcac tggctgacga
     7621 cagcaccttt gaatccgcag ttttctcaca aatccggcag atgctccatg cccggaattc
     7681 actggcggtt ttcggaagag gatcgatgga gtgggttcat ctgcggtcag gtgacggtaa
     7741 ccctgccgca agtgtgatgg cttacatccg ttccctggat ggtaaaaggg tactggtcat
     7801 ccataacctt tcagggaata ctgttccggt taaatgggag tttcccggtg gaaagacaat
     7861 cctcgggcct gatccggtaa ggaacggaga cttgatggaa ataagcggtt tcggatatca
     7921 ttggttgact ttgtaaatta ttttaagaag aggaatattt agaaatggat atgcaatctt
     7981 ttgatgcggt tatttttgac ctcgatgggg tgatcaccaa aacagcgctg gtgcatagtg
     8041 ccgcctggaa gaaaatgttc gacgggtatc tcagggagag ggaaataaaa tatggcgaac
     8101 ccttcaggga attttctcag aaagaagatt acctgcctta tgtcgacggc aagccgaggt
     8161 acaaaggagt cgagtctttc ctcgaatcga gagggataaa gctccctttc ggaaccccgg
     8221 aagatgatcc ggaaaaggag accgtttgcg ggatagggaa ccggaagaat atcgccttca
     8281 atgaagcgtt gaaatcggat ggcgtacagg tttacgaatc gacagtagcc ctgatccgcc
     8341 ggctgaaaga agaaggcatc cgcgtgggtg tggcttccag cagcaagaac tgccgccagg
     8401 tcctggaaac tgcaggatta ctcgatatgt tcgaaaccag ggtagacggg gaagtgtcgg
     8461 ccgaactggg actgaaaggc aaaccggagc ccgatatctt caccgtggca gccgataatc
     8521 tcggcgtgac ttacgaccgt gctgtggtcg tcgaagatgc cgtcagcggt gtccaggcag
     8581 gccgtaaagg caatttcggt ctcgtccttg gcctggcccg ggaagaaaat gaaaacgaac
     8641 tttattctaa tggagccgat atcgtggtca aagatatcgc tgatatcggc ctcgaaggga
     8701 ttgtcgactg gttccgccag gggcttttca aagacaactg gtcgctaact taccacgatt
     8761 acatccccga aagggaaaaa acccgtgaag cgctgcttac agttggaaac gggtatttcg
     8821 ggacccgagg cgccatgccc gaaaccatcg ccggtccata taactatccc ggaacctata
     8881 tggccggtat ctataaccgg ctggtctcca aagtcggcga taaggaaatt gaaaatgaag
     8941 actttgttaa tgtcatcaac tggctccctg ttactttcaa aatcaacgat gaggagtggt
     9001 tcgatgtgaa taatgccagg atcgctgaag tgcgccgcac gctccatttc aacaacggca
     9061 tgctttaccg caggctggtc gtgagagatg atgcaggccg cgaaacgctg atcgggaccc
     9121 gacggatagc cagcatggac aatccgaacc ttgccgcgca ggaatatacg gtcacacccc
     9181 tgaactattc cgctaaaatc actatacggt ccggtttgta cggcgatcat atcaatgccg
     9241 gcgtggagcg ctacagccag ttgaaccaga aacatatcga accggtgttg ctgggctcgg
     9301 aagagaatat ccagtatatg gtggttaaaa ccacccgttc gcaaaatgtc atcgcagccg
     9361 cggcacaggt gacggttttt gaaggaaacg accgggtcta tgtacatttt cataaaaacc
     9421 ataccgccgg ccgctcccgg atcgaattca cgaaaaaact ggcccaggga gaagctctgc
     9481 ggatacagaa gatcgtagcc ctttgccaca acagggacca ggacgaaacc gaccccctcg
     9541 attcggccat cgaaaattgt aaaaaattca ggaccttcga tgaagtcctc gagagatcgg
     9601 cccggaaatg ggaagagatc tgggaccgga tcgatatccg tgtcgccggc gaccgtctcg
     9661 cccaaaaact attgagactc catctgtacc ataccctgat caccacttca ccccttaacc
     9721 ggaatatcga tgcagggatt cccgcgcgcg ggctccatgg agaagcctac cgcggacata
     9781 tcttctggga tgaacttttt atcctccctt tctacaacct gcatttcagg gatgtggtca
     9841 aatcagtgct cctgtaccgg cataaaaggc tggacgaagc caggaaatat gccctggagt
     9901 atggatacca gggcgctatg ttcccctggc agagcggcag cagcggacgc gaggaaacgc
     9961 aaatcctcca ccttaaccct ctttccgggg agtgggggcc cgaccacagc tcgcttcagc
    10021 gccatgtttc gctggctatt gcctataata cctggcaata ctaccatatc accggcgacc
    10081 tggcatttat ggaagaatac ggcgccgaac tgatatttga gatctgccgt ttctgggcgg
    10141 ggaaaagcaa gctgaaccgc gtaaccggcc gttatgagat cattaaggtc atgggaccgg
    10201 atgagtttca tgaaaagtat gccgataccg atgaaggagg gctgaaagat aacgcttaca
    10261 ccaatatgat ggttgcatgg ctgttcggca gagctgccgt gttgttcgac cggatgagcc
    10321 cgcaggcaaa ggaaacggtc atggcaaaga ttcatctcgc taccggggag attgagcgat
    10381 ggaacgatat tgccgccagg ctgaacctgg tgatctctcc cgaagggatc atcgcacagt
    10441 atgacggtta tttcgacctg ctggaacttg attttgaaga gtatcgcaag aaatataaaa
    10501 acatctaccg gatggaccgg ctgctgaaag ccgaaggcaa atcgcccgac gaatacaagg
    10561 tggccaaaca ggccgatacg ctgatgactt tctacaacct aagcggtgaa gaggtcggtg
    10621 aaatcctcga aaagctgagc tataaactac cggatgatta cctgatgcgc aatctaaact
    10681 actacctggc ccggacctcc cacggttcca ccctcagccg ggtagtccat gcccaactgg
    10741 ccaacatgac gggcaacaaa agcctggcct gggaacttta ccaggacgca cttaccagcg
    10801 attatgccga tatccagggc ggaacgactg ccgaaggtat ccacgccggc gttatgaccg
    10861 gaacggtttg ggtagcattg gtctccttcg ccggattgaa cctgcatgga gattacccgc
    10921 gtttcaaccc gcaactgccg gcccactgga ggaagataag ctttttcttc agcttccgcg
    10981 acggcgagta cgaatgtgag gtgaccgctg ccggtattcg catacgggtt gataaacccg
    11041 aacccgggtc tgcacggatc ggattgcagg ataaaacatt tgaaatcaac accggcgaat
    11101 gggtcgacct ggagatttga aaatttgaaa atttggaaat ttgaaaattt gaaaatttgg
    11161 aaccctggaa ctttggaac
//
LOCUS       DAOPNH010000084        11103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6287_length_11103_cov_6.840424, whole genome shotgun sequence.
ACCESSION   DAOPNH010000084 DAOPNH010000000
VERSION     DAOPNH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6287_length_11103_cov_6.840424"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKZ74_05930"
     CDS             <1..763
                     /locus_tag="PKZ74_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPM92331.1"
                     /translation="GAITPGANASFVIMVVITCLASPLMYGQINRKKAYSDDHTIIIG
                     GSSVGVLLARRLKMLGKSSLIIENSPQRYNEIKSNGLDIIAGDGGDPAIYRSANLKPH
                     CYVVVLTANESQDMQICEMLRKELHHERIISIPGNTVIEDKMLGLGVHILDARRILAS
                     TIENIILRPGAYQSLIETFEEFSVEDITVLNGKIEGKKIMDLPLHKDAMIMLLTRGNE
                     KNVPHGNTYLKMGDVLTIFGTGTGIEEIRDVISRN"
     gene            complement(747..2288)
                     /gene="rny"
                     /locus_tag="PKZ74_05935"
     CDS             complement(747..2288)
                     /gene="rny"
                     /locus_tag="PKZ74_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPM92332.1"
                     /translation="MDVLYYLVDGLAALIIGIVIGTFILKRLNSKRFDAMRLEAEEKA
                     EVIKKEKILQAKEKFLELKAEHERAVNERNNAVLINENRIKQKESQISQKFEEIQRKQ
                     AELNTIRENLNNQLEIVSKKQAEYDRIIKEQVEKLETIAGLSAEEAKSQIVETLKEEA
                     KSDAMVYIKDTVEEAKITAQREAKKIVIETIQRTAAEHAIENTVSVFPLENDDIKGRI
                     IGREGRNIRALEALTGVEIIIDDSPEAIILSGFDPVRREIARLSLHKLVTDGRIHPAR
                     IEEVVAKTTKQIEQEVIEVGKRTCIDLGIHNMHNDLVRMIGRMKYRSSYGQNLLAHSI
                     EVAKLSATMAAELGLNPKMAKRAGLLHDIGKVPDNEPDLPHAILGMKMAEKFKEHPEI
                     CNAIGAHHDEIEMESLIAPIVQVCDAISGARPGARREVAEAYIQRLNKLEELALSYPG
                     VVKTYAIQAGRELRVIVGAEKVSDNEASKISYELSRKIQDEMTYPGQIKITVIRETRA
                     VNFAK"
     gene            complement(2581..2868)
                     /locus_tag="PKZ74_05940"
     CDS             complement(2581..2868)
                     /locus_tag="PKZ74_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPM92333.1"
                     /translation="MEELTITVNIADRPYRLKIKREEEEIIRKAVREIDARIKEYSEH
                     FSFNDKQDLVAMVLLHYASTAQKLEGDLVSNENMVVSRLQKMEELITHHLD"
     gene            complement(2894..3181)
                     /locus_tag="PKZ74_05945"
     CDS             complement(2894..3181)
                     /locus_tag="PKZ74_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92334.1"
                     /translation="MNPVTDHFQKLEQLIRQLAEDHNRNKSEKEILTRLSVTQQLTID
                     EQKTEIENLKKQVEALKLAKSVEHHEETGKVKEKIHDLVREIDRCIELLNK"
     gene            3433..4698
                     /locus_tag="PKZ74_05950"
     CDS             3433..4698
                     /locus_tag="PKZ74_05950"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPM92335.1"
                     /translation="MTEDFLHFVWKTRLRGEPYFTTAGESLVILKAGDHNHDSGPDFT
                     NAQVKIGETLWAGNVEIHIRSSDWTRHRHQADQAYDSVILHVVYEDDLPVRRRSGETI
                     PAFALKEILHPGLYETYQYFLNNHLWIPCAFRLPEVRPVIIHDWLTALTVRRLERKAL
                     ELENLLVYSGYDWNQAFFESMAATLGFRINRQPFEMMARSTPVSNLYKHKDNLFQVEA
                     MLFGQSGLLETRFRGDYPKRLRKEYSHLKNKFGLKPVSGHLWKFMRLRPNNFPTIRLA
                     QLAMLIHQREQFFGYILENNEYDRLRQFFRIGVSDYWMEHYYFDRPSKNTGKTISLST
                     VDLILINNVIPFLFLYGKLKGQPIFQDKAMELMDSIRPETNSIVRRFGDFGISPVSAA
                     QSQSLLELKSGFCDEKKCLDCRIGAELIR"
     gene            complement(4705..5250)
                     /locus_tag="PKZ74_05955"
     CDS             complement(4705..5250)
                     /locus_tag="PKZ74_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92336.1"
                     /translation="MRIIKVSFLILLLAIIWQGCTSDPKTRIRDLEKKIGSESFILDD
                     KGMALSGELIRSYLDFAESHKDDPSSPEFLYKAADLSLNTGQSKQSLDLYNRIIYQYP
                     NFEKVPECLFLVGYIYENYFQELGKAKSVYEAFLAKYPEHDFADDARVSIENLGKSPE
                     ELIRYFEEKNKALSDTLTSMK"
     gene            complement(5274..5852)
                     /gene="def"
                     /locus_tag="PKZ74_05960"
     CDS             complement(5274..5852)
                     /gene="def"
                     /locus_tag="PKZ74_05960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPM92337.1"
                     /translation="MILPIVAYGHPTLRKIAVEIEPGHPGLEELVENMFETMYSAEGV
                     GLAAPQVNQGIRLFIVDAAPYAEDHPELQGFKKVFVNPRILETEGEEIVFNEGCLSIP
                     KIREDVMRKPKVRMRYQDMQFNPFEETFEGITARIIQHEYDHLEATLFVDRINPLKKM
                     LLKRKLTDITNGNIDVDYKMIFPGKKYKGKGK"
     gene            complement(5857..6285)
                     /gene="ruvX"
                     /locus_tag="PKZ74_05965"
     CDS             complement(5857..6285)
                     /gene="ruvX"
                     /locus_tag="PKZ74_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPM92338.1"
                     /translation="MSRIMAVDYGTKRVGIAVTDESRIIATGLATVPSHEIFSFLTGY
                     LSREKVDVIVVGEPRQMNNKPSEALKFVIPFVNKLKKQFPDIEIDMMDERFTSKMAFQ
                     TMIDSGLRKKDRQNKGLVDRISATIILQSYLEYQSHKTNS"
     gene            6358..7176
                     /locus_tag="PKZ74_05970"
     CDS             6358..7176
                     /locus_tag="PKZ74_05970"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPM92339.1"
                     /translation="MYDQMRKIIESAWENREELKSVVTGRAIEEALSLLDRGKLRIAE
                     KAEGQWVVNEWIKKAVILFFPIRGMAVMEAGPMEFFDKIPLKKDYRSQGVRVVPHAVA
                     RYGSFMEPGVILMPSYVNIGAYIGEGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLE
                     PVQAAPVIIEDDCFIGSRCIVVEGVRVERESVLGAGVVLTSSTRIIDVSGNDAVEYKG
                     MVPAGSVVIPGTYPKTFPSGVFQVPCALIIGKRSASTDKKTSLNEALRNFQVAV"
     gene            complement(7201..7734)
                     /locus_tag="PKZ74_05975"
     CDS             complement(7201..7734)
                     /locus_tag="PKZ74_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92340.1"
                     /translation="MENLIFGIGSRVRHAKFGAGVVVQVWPDNYEIVFMDSGPKQIIH
                     GTEQLEVIDYRSPEPDQLSFDKVERILVKILRKFSDIQETVPLGDKWKGGKMIMQPAT
                     AGLQSKEVPIESFFHKIVMVRDRLRVLEQRVNASKLSDEEKIDIQQYITRIYGSLTTF
                     NVLFADKEDYFVGEKSV"
     gene            complement(7764..8363)
                     /gene="coaE"
                     /locus_tag="PKZ74_05980"
     CDS             complement(7764..8363)
                     /gene="coaE"
                     /locus_tag="PKZ74_05980"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPM92341.1"
                     /translation="MLRIGLTGNMGSGKTFVADIFQSFGIPVFFADKEARKLFSHDKI
                     KIQIRGVFGNEVFDKEGNISRSQLAAIVFRNNHLLAKLNRIIHPAVIEEYKNWLEHYP
                     DVPYTLYEAAILYESGHYSDMDRIICVTAPEAVRISRVITRDGISPEEAKIRMENQWD
                     ESRKAEMADFLIVNDGIKEVKLQVEQIHQQIILLTKSAS"
     gene            complement(8363..9361)
                     /locus_tag="PKZ74_05985"
     CDS             complement(8363..9361)
                     /locus_tag="PKZ74_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPM92342.1"
                     /translation="MKISLLELFQSAFGKRDDKFRQRLSIFLVCVGISCIIWFTIKLS
                     EEYDTVVEYPVRYTHLPKNKSLTFTSDSILQVEIIEKGSNLFRMLYVDKIEPVTINLR
                     FLPIYPKGGSYHGIVTPSLLINEIEREKGLLGKIISISPDTIYLTFEPEKSRKVPVKA
                     DFDLTFDKEYMRYGNSVFKPDCVTVKGPERVIENLDSVSLGYIKLENLTDSFTGNKKF
                     INDSINRTLSISPSEVSFIVPVEKYTETQVMVPVKPVNANGKNVRLFPDKVKVYCTVA
                     LKDYNRIQEGMIKAQADMSKADQSGDDKIKVSLENFPPFIRVNRIEPEKVEYIIVR"
     gene            complement(9407..9754)
                     /gene="yajC"
                     /locus_tag="PKZ74_05990"
     CDS             complement(9407..9754)
                     /gene="yajC"
                     /locus_tag="PKZ74_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPM92343.1"
                     /translation="MANLLYILLFAPPANPDGGSGSGSGSLLSFLPIILIILVFYLFF
                     IRPQMKKSKDQKKFREALKKGDKVITIGGLHGKILEVDETTVTLDVGNNMRLTFEKSA
                     IAMDTSSTIEQNK"
     gene            complement(9774..10229)
                     /locus_tag="PKZ74_05995"
     CDS             complement(9774..10229)
                     /locus_tag="PKZ74_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPM92344.1"
                     /translation="MRLINNLLILGIMAISACNGDPSRLPADVVNIPNSASGSVSENA
                     LPEMKFVKLEHDFGQVVMGEVVTYAFKFRNTGKGDLVIANISASCGCTASNFPKQPIK
                     PGEENFIEVSFNSEGRLGFQNKTLTVAANTQPGTIVLTIKAKVVSPELQ"
     gene            complement(10250..>11103)
                     /locus_tag="PKZ74_06000"
     CDS             complement(10250..>11103)
                     /locus_tag="PKZ74_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM92345.1"
                     /translation="HVYNDDSTFYCTDTVNLYIYPKVTIEYEQLVFGCPPADTNSVDT
                     ICKAQIKANVTGGYPPYQYDWGDSVLVNYGDSSWALNLCVDQTYVLIVKDTVCSYNLD
                     LYVEPFDMPDLEVTVEPDSLFETNPQAILSFENKSADSIPLTSWTWIFPDGTSTNQLT
                     PSYVFTTKDSLVKFTYTTIDNCNDTIYIPILVKEFELSVPNVFTPNGDGINDKYEIPY
                     LDRYISNEILIFDRWGTLVYKEKNYNSGWDGGRLPDGVYFYILKCQGYWEEDVFRGSV
                     SIYGSRY"
BASE COUNT         2777 a         2463 c         2527 g         3336 t
ORIGIN      
        1 tggggcaata acgccgggag ctaacgcaag ttttgttatt atggtcgtca tcacctgcct
       61 ggcatcgccc ttgatgtatg gacagatcaa caggaagaag gcctattcgg acgaccatac
      121 catcatcatc ggtggcagca gtgtgggcgt tttattggcc cgaaggctta aaatgctcgg
      181 aaaatcgtcg ttgatcattg agaatagtcc acaaagatat aatgagataa agtcaaacgg
      241 gctcgatatt attgccggtg acggaggtga ccctgccatc taccggagtg ctaacctgaa
      301 accgcactgc tacgtggttg ttctcacagc taatgaatcg caggatatgc agatatgcga
      361 gatgctccgg aaggagttgc atcatgaaag gataatatcc ataccgggga atacggtcat
      421 agaggacaag atgctgggtt tgggcgttca tatcctggat gcccgccgta tactggcttc
      481 taccattgaa aatataatcc tgcggccggg tgcttaccag tcgctcatcg aaaccttcga
      541 ggaattctct gtcgaagata tcaccgtttt aaacggtaaa attgagggga agaaaattat
      601 ggacctgcca ttgcataaag acgctatgat catgctcctg acgaggggaa atgaaaagaa
      661 cgtcccccat ggcaatactt atttgaagat gggagacgtt ctgaccattt tcggtaccgg
      721 aaccggtatc gaagaaatcc gtgatgttat ttcgcgaaat taactgcgcg ggtttcccgg
      781 atgacggtaa ttttaatctg acccggatag gtcatttcat cctggatttt ccttgacagc
      841 tcatacgaaa ttttactggc ttcgttatcg gaaactttct cagcgccgac gataacccgc
      901 aattcccgcc ccgcctggat agcgtaggtt ttcaccacgc ccgggtagga cagggccagc
      961 tcttcgagct tattgagccg ctggatgtaa gcttccgcca cttcgcgcct ggccccgggc
     1021 ctggcgccgg agatggcgtc acatacctgg acgatggggg cgatcagaga ctccatctca
     1081 atttcgtcgt ggtgtgcgcc gatggcgttg caaatttcag gatgctcctt gaacttttct
     1141 gccattttca tacccaggat agcatgtggc agatcgggct cattatcagg tacttttccg
     1201 atatcatgca acaaaccagc gcgtttggcc atcttgggat taaggcccag ttcagcagcc
     1261 atagtagcgc tcagtttggc cacctcgatc gaatgggcta aaaggttctg accatatgaa
     1321 gaacggtatt tcatccgccc gatcatcctg acaaggtcgt tgtgcatatt gtgaatgcca
     1381 agatcgatac aggttcgctt accgacctcg attacctcct gttcgatctg cttggtggtt
     1441 tttgccacca cttcttcaat acgggcagga tggattcgtc cgtcggtgac cagcttatgc
     1501 agcgataaac gggctatctc ccttctgacc ggatcaaaac ccgacaggat aatggcttcc
     1561 ggcgagtcgt cgatgattat ttcaacgcct gtcaaagctt ccagagcccg gatatttctt
     1621 ccttcacggc cgatgatccg ccctttgatg tcatcatttt ccagcgggaa aaccgaaacg
     1681 gtattttcaa tggcatgttc ggcagctgtt cgctggatcg tttcaattac gatctttttt
     1741 gcttcccgct gggccgtgat ctttgcttct tctaccgtat cttttatata aaccattgcg
     1801 tctgacttgg cttcttcttt aagtgtttca acgatttggg atttcgcttc ttccgctgaa
     1861 agtccggcaa tagtttcgag tttttctacc tgctctttga ttatgcggtc gtattcggcc
     1921 tgctttttag atacaatctc aagctggttg ttgaggtttt cccggatcgt attcagttct
     1981 gcctgttttc gttgtatttc ttcgaatttt tgtgagatct gggattcttt ttgctttatt
     2041 ctgttttcgt tgatcaggac ggcattgttt ctttcattga ccgctctttc gtgttcggcc
     2101 tttaattcca ggaacttctc tttggcttgc aggatcttct ctttttttat gacttcggct
     2161 ttttcttccg cttccaggcg catagcgtca aaacgtttgg aattcagcct tttcaaaata
     2221 aaagttccga tcacaatccc aatgattagt gctgccagtc catctactag gtaatataat
     2281 acatccatgg ttcactaacg attagattta tatgatttat ttgctaatgg tcaaaaaaat
     2341 acccgccatt tatccaagcg tttgggataa acccatttcg taacgcttag ggttgccccg
     2401 gcaggtcgaa cttctgctgt ccgccggctg gcggatcccg gttttcgccg ggttgcggcc
     2461 tgttctagct ccggtaagtc aaaccctgat gttgattgtg ttgatttatc aaaccagaat
     2521 tggataaatg cgggtaaatt tcgtatggtt aattttcaaa gaactatagg gcttgaattg
     2581 tcaatcaaga tggtgtgtga tcaattcctc catcttttga agccgcgaca ccaccatatt
     2641 ttcgttcgaa accaaatcgc cttccagctt ttgggctgtt gatgcgtaat gaagcaatac
     2701 catggctacc aggtcttgtt tatcattgaa agaaaagtgc tcggagtatt ctttgatcct
     2761 tgcgtcaatt tccctgacgg ctttacggat gatctcttct tcttctcttt tgatcttcaa
     2821 cctgtaaggc ctgtcggcta tattaaccgt gatggtcagc tcttccattt tccttacttt
     2881 gatttcgttg atcttatttg ttcagcaatt caatgcaccg gtctatttcc cgcaccaggt
     2941 catggatctt ttctttcacc ttccccgttt cttcatggtg ttcaacggat ttggccagtt
     3001 tgagcgcctc gacctgcttt tttaaatttt caatttctgt tttttgttcg tctattgtta
     3061 gttgttgagt gaccgaaagc ctggtcaaaa tttctttttc agatttattt ctattgtggt
     3121 cctcagcaag ttgcctgatc aactgctcca gtttttgaaa atgatctgtt acaggattca
     3181 tagcacatca agccgggttt tagttcaatc tttacaaaaa tatcaaaata attttacaat
     3241 gatacaggat gacaaaaata tgatgccgga ttttttctta tgatttatcc atcagcagac
     3301 atacggaaaa gattgaacta tccggattgt tggtactcaa tgaagtattt gtaaattaag
     3361 atattaggaa aatatttaat gatctccggg tattttttat ccattttccg gattaatatg
     3421 aaaagcgatg ttatgacgga agatttttta cattttgttt ggaagacaag gctccgggga
     3481 gagccgtact ttacaacggc aggggagagt cttgtcatcc tgaaagcggg tgatcataac
     3541 catgattccg ggcctgactt taccaatgcc caggtgaaaa ttggcgagac cttatgggcc
     3601 ggcaacgtcg aaatccacat ccggtcgtcg gattggaccc ggcaccgtca ccaggctgat
     3661 caggcttatg actcagttat tttgcatgtg gtgtatgagg atgacctgcc ggtcaggcga
     3721 cgcagtggag aaacaattcc tgcttttgcc ctgaaagaga tccttcatcc ggggttatat
     3781 gaaacatacc agtatttcct gaataaccat ttatggattc cttgcgcatt tcgtctgccg
     3841 gaggtcaggc ctgttatcat ccatgactgg cttacggcat tgacagtaag aaggttggag
     3901 agaaaagcgc tggaactgga gaacctgctt gtatattcgg gatatgactg gaatcaggct
     3961 ttttttgagt cgatggctgc cactctgggt ttcaggatca atcgccagcc atttgaaatg
     4021 atggcccgga gcacaccggt atccaatctc tataaacata aagacaatct tttccaggtg
     4081 gaggccatgc tctttggcca gtcggggctg ctggaaacac ggtttcgcgg tgattatccg
     4141 aaaaggctca gaaaggagta ttcacacctg aaaaacaaat tcggattaaa gcccgtttca
     4201 gggcacctct ggaaattcat gcggcttcgc cccaataatt ttcctaccat ccgcctagcg
     4261 caactggcta tgctgatcca ccagagggaa caattcttcg gctatatcct tgagaacaat
     4321 gaatatgacc gcctccgcca atttttcaga atcggtgtca gcgattattg gatggagcat
     4381 tattactttg accgtccctc aaaaaataca ggcaaaacca tttccctgtc aacagtcgac
     4441 ctgatcctga tcaataacgt cattcctttt cttttcctgt acggcaaact gaaagggcag
     4501 ccaatctttc aggataaagc catggaactg atggattcga tcaggccgga gacaaattcc
     4561 attgtgcgaa ggtttggcga tttcggtatc agtcctgttt ccgcagccca gtcacaatcg
     4621 ctcctggagc tgaaatcagg attctgtgat gagaaaaaat gcctggattg ccggattgga
     4681 gcggagttga tcaggtgaaa agggttattt catggaggtc agtgtatcac tcaatgcctt
     4741 gtttttctct tcaaaatagc ggatcagttc ttcagggctt ttgccaaggt tttcaatcga
     4801 aaccctggca tcatcggcaa agtcatgctc cggatatttc gcaagaaacg cttcatagac
     4861 cgattttgcc ttgccgagtt cctggaaata attttcataa atatagccaa ccagaaaaag
     4921 acattccgga accttttcga agttgggata ttggtaaatg atccggttat ataaatcgag
     4981 cgattgtttg ctctggcctg tattcagcga aagatcggcg gctttgtaaa ggaattcggg
     5041 tgaggaagga tcatctttat ggctctccgc gaaatcaaga taggacctga tgagttcgcc
     5101 ggataatgcc atccctttat catccaggat aaaactttca gaaccgatct tcttttccag
     5161 atctcttatc cttgttttcg gatcagaggt gcatccttgc cagataatgg ccagtagcag
     5221 gatcaggaat gatactttta tgattctcat aatcttcaat ttacacttta tgattacttt
     5281 cctttccctt tgtatttttt cccggggaaa atcattttat aatcaacgtc gatattgccg
     5341 tttgtaatat cggtcagctt tcttttcaac aacatttttt tcaggggatt tatcctgtcg
     5401 acaaacaggg tggcttccag gtggtcgtat tcatgctgga tgatccgtgc ggttatgcct
     5461 tcaaatgttt cttcaaatgg gttaaactgc atatcctggt atctcatcct gactttgggc
     5521 tttcgcataa catcttccct gattttggga atgctgaggc agccttcgtt aaaaacgatt
     5581 tcttccccct ctgtttcgag aatccgggga ttgacgaaga ccttcttaaa tccctgaagt
     5641 tccggatgat cctcagcgta aggtgcagca tctacaataa aaagccgtat gccctggttg
     5701 acctgaggtg cggcaagtcc aacgccttca gcagaataca tggtctcaaa catattttcg
     5761 accagttcct caagaccggg atgtcccggc tcaatctcca cggcgatttt tctgagtgtc
     5821 ggatggccgt aagctacaat aggcaatatc atcttcctaa gaatttgttt tatgtgattg
     5881 gtattcaaga taagactgta gaatgatggt ggcgctgatc ctatccacca ggcctttatt
     5941 ctgccggtct tttttcctga gccctgagtc gatcattgtc tggaatgcca tcttcgaagt
     6001 aaaccgttca tccatcatat cgatttcaat atcaggaaat tgtttcttta atttattcac
     6061 aaaagggatt acgaatttta acgcttctga cggtttattg ttcatttgcc tgggttctcc
     6121 cacaacgatc acgtctactt tttcgcggct cagataaccg gttaaaaatg aaaatatttc
     6181 atgagatggt acagtagcca gaccggttgc gataatcctg gattcgtcgg taaccgcaat
     6241 tccgactctt tttgtaccat aatcgacagc catgatccgg ctcatgaata atcagttaaa
     6301 attgaggagg caaaattacg aagaacttct ttatttttga tggataaacg gcaagctatg
     6361 tacgatcaga tgcgcaaaat cattgagtcg gcctgggaaa acagggaaga gctcaaatca
     6421 gtagttacag gcagggcaat agaagaagcg ttatcactcc tggaccgggg aaaattgcga
     6481 atagcggaga aggcagaggg gcagtgggtt gtaaacgaat ggatcaagaa agctgtgatt
     6541 ttgttttttc caatacgggg gatggcggta atggaagcgg ggccgatgga gtttttcgat
     6601 aaaattccat tgaagaaaga ttaccgtagc cagggtgtaa gggttgtacc gcacgcggta
     6661 gcccggtacg ggtctttcat ggaaccgggt gtgatcctga tgccgtctta tgtaaatatc
     6721 ggcgcatata tcggcgaagg cacaatggta gacacctggg caacggttgg gtcatgcgca
     6781 cagatagggc ggaatgtcca tttaagcggt ggtgtaggca tagggggggt gcttgaaccg
     6841 gtccaggcag cgccggtgat aatcgaagat gattgtttca ttggttccag gtgtattgtg
     6901 gttgaagggg tgagggtgga aagggagtcg gtgctgggag ccggggttgt gctgacctct
     6961 tcgacccgga tcatcgatgt ttcagggaat gatgcggtgg aatataaagg gatggtgccg
     7021 gcagggagcg tcgtcattcc gggaacctat cccaagacat tcccttctgg cgtattccag
     7081 gtgccttgtg cattgatcat cggaaaacgg agtgcttcta ccgataagaa aacatcgctg
     7141 aatgaagcgc tgaggaattt tcaggttgca gtatgatcgg tttttctttc tttgtctgag
     7201 tcaaacactt ttttcgccga cgaaataatc ttctttatcc gcaaacaaga cattaaatgt
     7261 ggtcagactg ccatagattc gggtaatata ctgctgaata tcaatctttt cctcgtccga
     7321 tagtttactg gcgttcaccc tttgttccag aaccctgagc cggtcgcgaa ccatcacaat
     7381 tttatggaaa aagctttcga taggtacttc tttgctctgc aaaccagctg tcgcaggctg
     7441 catgatcatt ttgccgcctt tccatttatc gcccagaggc acagtttcct gtatatcaga
     7501 aaatttgcga agaattttaa ccaatattcg ttcgaccttg tcgaaactta gctggtcggg
     7561 ttcaggactt cggtaatcga tcacttccag ttgttctgtt ccatggatga tttgttttgg
     7621 tcctgagtcc atgaaaacga tctcataatt gtcaggccac acctgaacga ccacaccggc
     7681 gccgaatttg gcatgccgca cacgcgatcc gattccgaat atcaagtttt ccataatttt
     7741 tattcaaatt agccggttga tcactaagag gcactttttg tgagcaatat gatttgttga
     7801 tgtatttgct cgacttgcaa tttaacttct tttatcccgt cattaactat taaaaaatca
     7861 gccatttctg ccttacggga ttcatcccac tggttttcca tccgtatctt tgcttcctcc
     7921 gggctgatgc cgtcacgggt aataaccctg ctgatcctta ctgcttccgg tgcggtaaca
     7981 cagatgatcc tgtccatatc gctatagtga ccgctttcat aaagaatggc agcttcatat
     8041 aaggtatatg gtacatcagg ataatgttcc agccagtttt tatattcttc tataaccgcc
     8101 ggatgaataa tccggttaag ttttgccagc agatggttgt tcctaaaaac gatcgcagcc
     8161 agctgcgatc tactgatatt tccttcttta tcaaatactt cgtttccaaa tactcccctg
     8221 atctgaattt tgattttatc gtgactgaaa agtttcctgg cttctttatc cgcaaagaac
     8281 actggaatgc caaaggattg aaagatatca gcgacaaacg tctttccact tcccatattt
     8341 ccggtaagac cgatcctgag cattacctga caataatata ttccaccttt tcgggttcaa
     8401 tccggtttac ccggatgaaa ggcgggaaat tttccagact cacttttatt ttgtcgtcgc
     8461 ccgactgatc ggctttcgac atatctgcct gtgccttgat catgccctcc tggattctgt
     8521 tgtaatcttt aagtgcaacg gtgcaatata cttttacttt atccgggaat aacctgacat
     8581 tctttccatt cgcattaacc ggtttcacag gcaccattac ctgcgtctcg gtatattttt
     8641 ctaccggtac gataaagctg acttccgatg ggctgatcga cagagtgcgg ttaatactat
     8701 cattgataaa tttcttattt cctgtaaatg agtcggttag attttccagt ttgatataac
     8761 ctaaagacac ggaatcgagg ttttcaatca cacgctccgg accttttact gttacgcaat
     8821 cgggtttaaa gaccgagttt ccgtaacgca tgtactcttt gtcgaaggtg agatcgaaat
     8881 cggcttttac agggactttc cttgattttt cgggttcaaa agtcagatat atggtgtcgg
     8941 gactgatgga gatgatctta cccagaagcc ctttttccct ctctatttca ttgattaaca
     9001 gcgatggcgt gacaattccg tggtaagatc cccctttcgg atatataggc aaaaacctga
     9061 ggttaatagt cacaggctct attttatcca cataaagcat tctgaaaagg ttggatccct
     9121 tttcaattat ttcaacctgg aggattgagt cggatgtgaa cgtaagtgat ttatttttag
     9181 gaagatgggt ataacggacc gggtattcaa ccaccgtatc atattcctcc gacagtttga
     9241 tcgtaaacca gataatgcag gagatgccga cacaaaccag aaaaatggat aaccgttgcc
     9301 ggaatttatc atcccttttc ccaaaagccg attgaaaaag ttctaaaaga cttatcttca
     9361 aatctttaaa gattgcctgg agttatcgta aaaacgtaac gacggactac ttattctgtt
     9421 cgatggttga tgacgtatcc atagctatag ctgacttttc aaaagtcagg cgcatattgt
     9481 tgccgacgtc cagtgtgacg gtagtctcgt ccacttccag gattttcccg tgcaatccac
     9541 cgatagtgat caccttatct ccttttttca atgcttcccg gaatttcttt tgatccttgc
     9601 tttttttcat ctgcggacga ataaagaaaa gataaaagac caggatgatc aggataatgg
     9661 gtaaaaacga aagcaggctg ccggagccgg agccggaacc tccatccggg ttggctggag
     9721 gagcgaataa taagatgtaa agtaaatttg ccatatgttc taaaaattaa ttgttattgt
     9781 aattccggtg aaaccacttt tgctttgatt gtcaggacaa tagtgccggg ctgggtattg
     9841 gcagcgacgg tcagagtttt gttttgaaat ccgagccggc cttcgctgtt gaagctgact
     9901 tcaataaagt tttcttcccc gggcttgatg ggctgtttcg ggaaattgga ggcggtacag
     9961 ccgcaggatg cgctgatgtt ggcgatcaca aggtcgcctt ttcccgtgtt cctgaattta
    10021 aaagcatacg taactacttc acccataaca acctgaccaa aatcatgttc cagtttgacg
    10081 aatttcattt ccggcagggc attttcactg acggaaccag acgctgagtt aggaatattt
    10141 accacatcag ccggcaaacg tgacggatct ccgttgcagg cgctgatggc catgattccc
    10201 agaatgagta aattattaat gagtcgcata atatttcagc cttaaaaagt cagtatcggc
    10261 tcccgtaaat ggatactgaa cccctgaaga catcttcctc ccagtatccc tgacatttta
    10321 aaatataaaa gtaaacaccg tcgggcaatc tgccgccatc ccatccgctg ttatagttct
    10381 tttctttgta taccagtgtt ccccatcggt caaagattaa tatttcattt gaaatatacc
    10441 tgtcaagata ggggatttca tatttatcgt tgattccatc cccgttcggt gtaaagacat
    10501 tcggcacact cagttcaaat tccttgacca ggatgggtat ataaattgta tcgttgcaat
    10561 tgtcgatcgt ggtataggta aatttgacca gcgaatcctt cgtcgtaaaa acatagctgg
    10621 gagtcagctg atttgtggat gttccgtctg gaaatatcca ggtccaggag gtcaggggta
    10681 tcgaatccgc cgatttattt tcgaaagaga ggatagcctg cggattggtt tcgaacaggc
    10741 tgtcgggctc tactgttact tcaaggtcgg gcatgtcgaa aggttccacg tagaggtcca
    10801 ggttatagct gcaaaccgtg tctttcacga tcaggacata ggtctgatca acgcagagat
    10861 tcagggccca tgaagaatcg ccataattca ccagtacgga gtcgccccag tcatactggt
    10921 acggtggata gccgcctgtt acattggctt ttatctgggc cttacatatc gtatcgacac
    10981 tattggtatc ggctggcggg catccgaaga ccagttgttc gtattcgatc gtgaccttag
    11041 gataaatata aagattgacc gtatcggtac agtagaatgt gctgtcgtcg ttgtagacgt
    11101 gca
//
LOCUS       DAOPNH010000085        11010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6360_length_11010_cov_5.794432, whole genome shotgun sequence.
ACCESSION   DAOPNH010000085 DAOPNH010000000
VERSION     DAOPNH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_11010_cov_5.794432"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1320)
                     /gene="mraY"
                     /locus_tag="PKZ74_06005"
     CDS             complement(79..1320)
                     /gene="mraY"
                     /locus_tag="PKZ74_06005"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPM92346.1"
                     /translation="MLYYLADYLQRCCDVPGAGVFQYISFRTAAAVIVSLLISLVFGT
                     KWIRYLNKRQVGETIRDLGLEGQLAKQGTPTMGGLIILASILIPTILFAKLDNIYITL
                     MLFTTVWLGSVGFLDDYIKVFKKNKNGLRGKYKILAQVILGIAVGATMFFSDGVVIRE
                     KVTQTSPSTEEITTSTGVSEKFSAVSVKSTKTTIPFVKNNEFDYAVLLRWLGTGYEKY
                     AFLIFIPLVIFIITAVSNGANLTDGMDGLATGTSAIIGATLGLLAWVSGNIIFADYLN
                     IMYLPNTGELTIFIGAFVGGCIGFLWYNSYPAQVFMGDTGSLALGGIIAVFAIIIRKE
                     LLIPILCGIFVMESLSVVMQVAYFKFTRKRFGEGRRIFKMSPLHHHFQVLGYPEPKIV
                     QRFMIVGIILAVITVITLKLR"
     gene            complement(1323..2795)
                     /locus_tag="PKZ74_06010"
     CDS             complement(1323..2795)
                     /locus_tag="PKZ74_06010"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPM92347.1"
                     /translation="MKKLSDILYKAGVVRVVGNPHARIGEIRFDSRLVGKGDLFVAVR
                     GTQADGHAFIGKAIESGAAVVVCEEMPGTLIPGITYAQVTDCSFALGVMASNYFDNPS
                     EELTLVGVTGTNGKTTIATLLYQVFTGLGYGCGLLSTIRNVIMGKVIAATHTTPDPLA
                     LNALLRQLADEGGQYCFMEVSSHAVAQNRIAGLNFAGGIFTNLTHDHLDYHKTFKDYL
                     LAKKKFFDLLPSRAFALVNKDDKNGMVMVQNTTARIRTYAVKSPADYKCRITENLLEG
                     LQLTINETEIYCRLTGEFNAYNLTAVFGTALLLQQPAENVLTFLSSVPPVEGRFDTLH
                     SDNGITAIVDYAHTPDALKNVLTTIEEIRTRNEKLITVVGAGGDRDRSKRPLMGGICA
                     AKSDLVILTSDNPRSEEPEEIIAEMQAGISAELKRKVMVIVNRREAIRTACHLAKPGD
                     IILVAGKGHEKYQEIKGIRHHFDDKEILEEMLLNENQKPI"
     gene            complement(2792..4915)
                     /locus_tag="PKZ74_06015"
     CDS             complement(2792..4915)
                     /locus_tag="PKZ74_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPM92348.1"
                     /translation="MKDIKRDILWRVYLVYFGVLVFALVIIYRAAYLQFSMKDELLEK
                     AKKQEVKYFPIEALRGNILARDGSLLASSIPDFEIRMDLSPNVVQPEIFNAKIDSLSM
                     LLAGLFKDKSSLDYKSDLLRARADGNRYFLVKNHVKYDELKKLKRFPIFRLGKYAGGL
                     IVIRKTTRELPYKELARRTIGFENQTESLFVGLEGAYSEDLKGHEGLQLKRRISQGEW
                     IPVNDNPEVAPRDGRDIITTIDVNIQDLAESALARHLEEHKAFQGCAILMEVSTGEIR
                     AIANLRYDPKDGKFKEIYNYAVGETIEPGSTFKLPSLIAALETGKIRLSDQYYVGNGR
                     VQYHNRWMLDSHRPESDHMSVQRIFEESSNVGVSKIITTLFGAHPEEYVSLLRKMSLG
                     QQLGVDIPGEGKPYLKDPSDKQHWYGTSLPWMSIGYELTLTPLQILTLYNAVANNGVM
                     VKPIFVSEIREGGKTIRKNEISVLNPSICSPSTLTQVKYLLEGVVENGTGSVLKDSTY
                     RIAGKTGTALVADGEKGYSEKKYNASFVGYFPADNPKYSCIVVVNRPEAGKIYGGAVA
                     APVFKEIADKVYATQMDIHDQTEKTEYGKIHLPDRGKGNYQDLKTSLVALSYPVMMPS
                     QTPEWVQMDSSVVGIVLKPAYYGIDTIPDMIGMTGKDAVYMMEKAGLKPVIHGKGLVS
                     EQSLLAGTWLEKGSRIELFLSDTIR"
     gene            complement(4912..5319)
                     /locus_tag="PKZ74_06020"
     CDS             complement(4912..5319)
                     /locus_tag="PKZ74_06020"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPM92349.1"
                     /translation="MDSRGEKRSATFFKLPGKEVFSRRHIGQSIHSILDGTFLTRERF
                     MRALPFIIFLIILGIMYITNIFHVEKTKRQIDDLEEELRELRYEYITSRSRVMFESKS
                     SEIARKLKDTGISESMHPPKKIIIGEKDKISMP"
     gene            complement(5321..6205)
                     /gene="rsmH"
                     /locus_tag="PKZ74_06025"
     CDS             complement(5321..6205)
                     /gene="rsmH"
                     /locus_tag="PKZ74_06025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPM92350.1"
                     /translation="MYHIPVLLKESVDGLDIRPDGIYVDATFGSGGHSGKILEKLVTG
                     KLFAFDADRDALANAPEDARFELIGHNFRYLKNFLRIHGITRIDGILADLGISSWQID
                     QPDRGFSTRFSGKLDMRMDREQDKDAWQVVNGYSLEDLTIVLKKYGEVENAFRLSQEI
                     ILSRPVNTTEEFKSLVSRRAPKGKENQYLAKVFQAIRIEVNDELEALKELLQHGTDLL
                     HPGGRFAVISYHSLEDRLVKNYFKTGNFEGNPVKDFYGNLIAPLAPVTRKAIIPSASE
                     QSVNSRSRSAKLRIAKKI"
     gene            complement(6205..6672)
                     /gene="mraZ"
                     /locus_tag="PKZ74_06030"
     CDS             complement(6205..6672)
                     /gene="mraZ"
                     /locus_tag="PKZ74_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPM92351.1"
                     /translation="MLNIIGTYECRVDAKGRLMFPVAFKNQLSQVVAQGFVIKRSIFR
                     NCLELYPVPEWNAESARINKLNRFRKKNVDFIRKFMAGVKLTELDSTGRLLIPKDLFN
                     FAAIKQDVVMASVVNKIEIWDKDEYEKAVSYDAEEFANLAEEVMGDNDVEPIE"
     gene            6913..7788
                     /locus_tag="PKZ74_06035"
     CDS             6913..7788
                     /locus_tag="PKZ74_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HPM92352.1"
                     /translation="MENTLQRKARESQPVITEKYIDVRKIFRSKNPRLAGSIPGFIFR
                     LLEKIIHQDGINDFMYRNRDKWGLDYAEAILDGFRVSTRVVNPPDLSHGRYLIASNHP
                     LGGMDGIALIHETGKLKKDLLFPVNDLLLNLPNLYELFIPVNKHGSNAENIRLFNDTF
                     ASDVLILYFPAGLVSRKQAGEIKDVEWKKTFLTKARQSGRDIVPVHMAGRNTEFFYNL
                     ANFRKRLGIKANIEMLFLPDELFKYQGKEIVITYGNPIPIGTFNRSKSDQEWASLLRE
                     HVYKLERNPNEIFNY"
     gene            7811..8737
                     /locus_tag="PKZ74_06040"
     CDS             7811..8737
                     /locus_tag="PKZ74_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM92353.1"
                     /translation="MQAVIPPVNKALLEAELTPEKFVRKTNNGNNLIYIFDYDSCPNL
                     MREIGRLREITFRDSGGGTGKDNDIDEFDLGDKPFHQLIVWNPEDKEIVGGYRFAQGD
                     RLKINDKGEVQSPTAELFEFSDKFVKDYLPWGIELGRSFVQPNYQPQFNLRKGMYSLD
                     NIWDGLGAIVIDNPGYKYFFGKLTMYPHYEYFARDMVLYFLQKFFPDPEGLMTPREPL
                     HLRHDKDYLDSFFTGSNYVENYKILVQQVRSHNETVPPLVNAYMSLSATMKTFGTSLN
                     RHFGDVEETGIIITIGDIYDFKIDRHIATYKK"
     gene            8737..9354
                     /gene="yihA"
                     /locus_tag="PKZ74_06045"
     CDS             8737..9354
                     /gene="yihA"
                     /locus_tag="PKZ74_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPM92354.1"
                     /translation="MATNPLSISARFITSSPEYTKCPAPAMPEYAFIGRSNVGKSSLI
                     NMLTGVKMLAKTSSTPGKTQLINHFLINESWYIVDLPGYGYASTAKIKREQWPNVIEK
                     YLLYRTSLMTTFLLIDSRLELQENDRKVINWFGENGLPFVIVLTKTDKLTPNQVNASQ
                     LRLANKLKEEWEELPVFILTSSKSLEGRTEILQYIDQTNLLFQVP"
     gene            complement(9369..9719)
                     /locus_tag="PKZ74_06050"
     CDS             complement(9369..9719)
                     /locus_tag="PKZ74_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPM92355.1"
                     /translation="MTEHKHKQADENSQQPQYGSMLLDDFTYQTIITEKFRNRKPYEP
                     EDPKKIFAFIPGKIRKVHIKKGSKVKEGEILLVLEAMKMNNNIFSPLKGVIKEVYVTP
                     GLSVAKGTLLAEFR"
     gene            complement(9730..>11010)
                     /locus_tag="PKZ74_06055"
     CDS             complement(9730..>11010)
                     /locus_tag="PKZ74_06055"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPM92356.1"
                     /translation="IFAQDFTVAGGSLGYMHAKKITKIMDHAMKLKVPLIGINDSGGA
                     RIQEGVNSLAGYGEIFYRNTQASGVIPQISVILGPCAGGAVYSPALTDFVFVVDKISK
                     MFITGPEVIKATIGEEISMEDLGGARVHAEKTGNAHFFAESEQECFDQIKQLITFIPW
                     NNQKRADSFPKRPPKSRSFKIDQIIPSDPKQPYDVRDVIKALADDSEFLEIQELWARN
                     IVIGFARVDGETVGCVANQPLYLAGVLDVDSSDKAARFIRYCDAFNIPLLTLVDLPGY
                     LPGIDQEHAGVIRHGAKLLYAYSEATVPKITIILRKAYGGGYIAMCSHHLKADFVFAW
                     PTAEIAVMGPEGAANIIFRNEIKNSDNPEATRKLKIKEYKEKFANPYVAAAYGYIDAV
                     IEPSETRNMLVHALQISMQKQETPPKKKHGIPPF"
BASE COUNT         2648 a         2754 c         2657 g         2951 t
ORIGIN      
        1 ctttcagccg tgccaggact gccgccccgg cgccgctttc gcctcctccc agtataatga
       61 tatcgtgacg gttcatcttt atctcagttt taatgtgatg acggtgatga cggccaggat
      121 gatcccgacg atcatgaacc gttggacaat ctttggttcc ggatagccca gcacctggaa
      181 atgatgatgc aggggcgaca ttttaaaaat cctgcgacct tctccgaacc tcttccgtgt
      241 gaatttgaaa taggcaacct gcatgacgac cgaaaggctt tccatcacga atatcccgca
      301 aaggatgggg atcaggagct ccttacggat gataatggca aaaacggcaa tgatcccgcc
      361 cagggccaga ctccccgtat cgcccatgaa aacctgcgcc ggataggaat tgtaccagag
      421 gaatccgata cagcctccga cgaaggcccc gatgaatatg gtcaactcgc cggtattggg
      481 caggtacatg atattcaggt aatcagcaaa gatgatgttg cccgataccc aggccagcag
      541 cccaagagtg gccccgatga tggccgaggt gccggtagcc agcccgtcca tgccgtcggt
      601 aagattggcg ccgttggata ctgccgtaat gatgaaaatc accagcggta taaaaatcag
      661 gaacgcatac ttttcgtagc ctgtgcctaa ccacctaagc aatacggcat aatcaaattc
      721 attgttcttg acgaatggaa tggttgtttt ggtcgatttg accgataccg cgctgaattt
      781 ctccgaaacg ccggtggagg tagtgatctc ttcggttgaa gggctggtct gtgtaacttt
      841 ttcccggatg acaacgccat cgctgaagaa catcgtggca ccgacagcaa tgccaagaat
      901 gacctgagcc agtattttat attttcccct taaaccattc ttgttttttt tgaaaacttt
      961 aatgtaatcg tccagaaagc cgaccgagcc aagccataca gtcgtgaaca acatcagggt
     1021 aatgtaaatg ttgtcgagtt tggcgaacag gatagtcggg atgaggatgg aagccaggat
     1081 gatgagcccg cccatggtcg gggttccctg cttggccagt tgcccctcca ggcccaggtc
     1141 gcggatcgtt tccccgacct gccttttgtt caggtatcta atccatttcg ttccaaaaac
     1201 cagcgagata agcaaagaaa cgatcactgc cgccgccgta cggaaggaga tgtactggaa
     1261 aaccccggct cccggcacat cgcaacatct ttgcaaataa tcggctaagt agtacagcat
     1321 gattatatgg gtttttggtt ttcgttcagt aacatctctt ccagaatctc cttatcatcg
     1381 aaatgatgcc ggataccttt tatttcctgg tatttttcat ggccctttcc ggcaaccagg
     1441 ataatatcgc cgggctttgc cagatggcag gccgtccgga tcgcttccct ccggttgacg
     1501 atcaccatca ctttccgctt caactcggcg gaaatgcccg cctgcatttc ggcaatgatc
     1561 tcttccggct cctccgaccg cggattgtct gaagtgagaa tcaccagatc gcttttggct
     1621 gcgcagatgc ctcccatcaa aggccgcttc gagcggtcac ggtcaccgcc tgccccgacc
     1681 acagtgatca gcttctcatt gcgggtccgg atctcttcga tggtggtcag cacatttttc
     1741 aatgcatcgg gtgtatgggc gtaatcgaca atggccgtga tgccgttatc tgagtgaaga
     1801 gtgtcgaaac ggccttctac cggcggcacc gagctgagaa atgtcagcac attttcggcc
     1861 ggctgttgta gcaggagtgc cgtgccgaag acggctgtca ggttgtaggc attgaattcc
     1921 cccgtcagcc tgcagtaaat ttccgtttcg ttaattgtga gctgcagccc ttccagcagg
     1981 ttctctgtga tccggcattt gtaatcggcc ggtgatttaa cggcgtaggt cctgatccgt
     2041 gcagtggtat tctgtaccat caccattccg ttcttgtcgt ctttgttgac cagcgcaaaa
     2101 gcccgggagg ggaggaggtc gaagaatttc ttcttggcca gcaggtaatc tttgaatgtt
     2161 ttatggtaat ccaggtgatc gtgagtcagg ttcgtgaaaa tgcccccggc aaagttcagc
     2221 ccggcgatgc ggttttgtgc gacggcatgg gaactcacct ccatgaagca gtattgcccg
     2281 ccttcatcgg ccagttgcct gagtaaagcg ttcagggcca gcggatcggg cgtcgtatgg
     2341 gtggcagcga tcaccttgcc catgataacg ttccgaatcg tcgataacag gccgcagcca
     2401 tagcctagcc cggtaaatac ctggtacaga agggtagcga tcgtggtttt gccgttagta
     2461 cccgtcacgc ctaccagcgt aagttcttcg gaaggattat caaaataatt ggaagccata
     2521 acccccaggg caaaactgca gtcggtaacc tgggcatatg ttattccggg tatcaatgtc
     2581 ccgggcatct cttcgcaaac gaccacggca gcgcccgatt caatggcctt gccgatgaaa
     2641 gcgtgaccgt cggcctgcgt tcccctgacc gccacgaaaa gatccccctt tccgaccaac
     2701 ctcgaatcga agcgtatttc accgatccgg gcatgcgggt ttcctacgac cctgaccaca
     2761 ccggccttgt acaatatatc gctcagtttc ttcacctgat cgtatctgaa agaaataatt
     2821 caatccggct ccctttctcc agccaggttc ctgccagcaa tgattgttcg cttaccagcc
     2881 ctttcccgtg gatgaccggt tttaaacctg ccttttccat catgtagacg gcgtcttttc
     2941 cggtcatgcc gatcatatcc ggtatcgtgt caattccgta ataggcgggt ttcagtacga
     3001 tacccaccac ggaactgtcc atttgtaccc attcgggtgt ctgcgatggc atcatgaccg
     3061 ggtatgacaa agcgacgagg cttgttttca ggtcctggta attgcctttg ccccgatcgg
     3121 gaaggtgaat ctttccgtat tccgtttttt ctgtctggtc gtggatatcc atctgcgtgg
     3181 catagacttt atcggcaatc tccttaaaaa caggggctgc cacagctccg ccgtatatct
     3241 tccccgcttc cggccggttt acaacgacta tacaggaata cttgggattg tcggccggga
     3301 agtagccaac aaatgaggca ttgtatttct tttcggaata gcccttctcg ccatcggcaa
     3361 ccagcgctgt gcctgtcttt ccggcaatcc ggtacgttga gtctttcaaa acactgcccg
     3421 tcccgttctc cacgacaccc tccaggaggt acttgacctg tgtcagggtt gacggcgagc
     3481 agatagaggg attgagcacc gatatttcgt ttttccggat ggtttttccc ccttccctga
     3541 tttcgctgac gaagatcggt tttaccatta cgccattgtt cgccacggca ttataaaggg
     3601 tcaggatctg taatggggtg agggtcagtt catatccgat cgacatccag ggcagactgg
     3661 taccgtacca atgttgcttg tcggagggat ccttcagata cggtttgcct tctcccggaa
     3721 tatccactcc gagctgctga ccgagcgaca tcttccggag cagtgaaaca tattcttccg
     3781 ggtgagcacc gaacagggtt gtgatgattt tggatacgcc gacattggat gactcttcga
     3841 atattcgctg aacgctcatg tgatccgatt ctggacgatg cgaatcgagc atccagcgat
     3901 tatggtactg aacccggccg ttgccgacgt aatactggtc cgaaagcctg atcttgccgg
     3961 tttcaagtgc cgctatgagg gagggtagct tgaaagtgga ccccggttcg atggtttccc
     4021 cgacggcata gttgtagatc tccttgaact ttccgtcttt cggatcgtag cgaaggttgg
     4081 cgatggcccg gatctcaccg gtgcttacct ccatcaggat cgcacagccc tgaaacgctt
     4141 tgtgctcctc caggtggcgc gccagggccg attcggccag gtcctgtata ttcacgtcga
     4201 tcgtcgtaat aatatcgcgt ccatccctgg gggctacttc cggattgtcg ttcaccggga
     4261 tccattcccc ctggctgatc cgtcgtttca gttgcaggcc ttcatggcct ttgaggtcct
     4321 cgctgtaggc cccttccagc cccacaaaaa ggctttcggt ctggttttca aaaccgatgg
     4381 tccgacgcgc aagttctttg taaggcaatt cacgtgtcgt cttccggata acgatcaatc
     4441 ccccggcgta tttacccagg cggaagatcg gaaatctctt cagtttcttt aattcatcat
     4501 atttaacatg gtttttgacc aggaaatagc ggtttccgtc ggcccgcgca cgaagcaggt
     4561 ctgatttgta atccagtgac gatttatctt tgaataaccc ggcgagcagc atcgaaagcg
     4621 aatcgatctt tgcattgaat atttccggct gaacgacatt gggcgaaaga tccatacgga
     4681 tttcaaaatc gggaatcgat gaagccagca ggctgccgtc gcgcgccagg atgtttccgc
     4741 gtaaggcttc gatggggaag tatttcacct cctgtttttt tgctttttca agcaactcat
     4801 ccttcatgga aaattgaaga taagccgcac ggtagatgat taccagcgca aaaaccagta
     4861 ccccgaagta aaccaggtaa acccgccata gtatgtccct ttttatatct ttcatggcat
     4921 gctgattttg tctttttcac cgatgatgat ctttttcggc ggatgcatcg attcgctgat
     4981 ccctgtatcc ttaagcttcc tggcaatttc cgatgatttg ctctcaaaca tgacccggct
     5041 gcgggatgta atgtattcgt accggagctc tctcagttcc tcttccaggt catcgatctg
     5101 acgtttggtc ttctctacat gaaaaatatt ggttatatac atgataccca atatgataag
     5161 aaagatgatg aaaggaaggg cccgcatgaa cctttccctt gttaagaagg tgccatcgag
     5221 aatggaatga atcgactggc cgatatgcct ccggctgaat acctccttgc cgggtaattt
     5281 gaagaatgtt gccgatcgtt tttctcccct gctatccata tcagatcttt tttgcaattc
     5341 tgagtttagc gcttctcgac cggctgttga ccgattgttc tgaggctgaa gggatgatgg
     5401 ctttgcgcgt tacaggcgcc aggggggcaa tcaggttgcc atagaaatct ttcaccgggt
     5461 tgccttcgaa attgcccgtt ttaaagtaat tcttcaccag tctgtcttca aggctgtggt
     5521 aggagatcac ggcgaaacgt ccgcccgggt gtaacaggtc tgttccatgc tgtaataact
     5581 ctttcagggc ttccagttca tcgtttactt cgatccggat ggcctggaag actttggcca
     5641 ggtattgatt ttccttgcct ttgggagccc gccgtgaaac cagcgacttg aattcctccg
     5701 tggtgttgac cggacggctg aggatgattt cctgcgaaag cctgaaggca ttttctactt
     5761 ccccgtattt tttcaggact attgtcaggt cctcaaggga gtaaccgttc acaacctgcc
     5821 atgcatcttt atcctgctcc cggtccatcc gcatatcgag ctttccgcta aagcgggtgg
     5881 aaaatcccct gtcaggctga tcgatctgcc aggaggagat acccaggtcg gccaggatac
     5941 cgtcgatccg ggtgatacca tggatccgga ggaaattctt caggtacctg aaattatggc
     6001 cgatcagttc gaaccgcgcg tcttccgggg cgttggcgag tgcgtccctg tctgcatcga
     6061 aggcgaaaag cttcccggtg accagctttt ccaggatctt gccggagtgg ccgccactgc
     6121 caaaagtggc gtcgacataa atcccgtcag gacgtatgtc aaggccatca accgattctt
     6181 tcaacaggac tggaatatgg tacatcactc tatgggttca acatcgttat cgcccatgac
     6241 ctcttcggcc aggtttgcga attcctcagc gtcgtacgaa accgcttttt cgtactcgtc
     6301 cttatcccag atttcaattt tattgacaac cgatgccatc accacatcct gctttatcgc
     6361 ggcgaaattg aacaggtctt tcgggatcag cagcctgccc gttgagtcaa gttctgtcag
     6421 tttgacgccg gccatgaatt tccggatgaa atccacgttc ttctttcgga aacggttgag
     6481 tttattgatc cgggcacttt ctgcattcca ttccgggaca ggatacagct caaggcagtt
     6541 gcggaaaatg ctccgcttga taacaaagcc ctgggccacc acctgcgata actggttctt
     6601 gaaagccact ggaaacatga gccttccctt ggcatcaacc ctgcattcgt atgtcccgat
     6661 aatgtttaac aaggatcaga ggaattttga tgtaaatata gagttgtttt taccacattt
     6721 taccacttta taccactttt taactaacaa ggtttcaatt tttattaaca cattttgatg
     6781 ataactttta aataattgaa tgacagttgc ttacgggcac atttataatc cggcgatcaa
     6841 ccgggcccta aatttgacaa ctgtatccgc aaacaccgat tatttcctat atttgttctg
     6901 gaaagaggca ttatggagaa tacccttcaa cgaaaggcaa gagaaagcca gccggtgatt
     6961 actgagaaat acatcgacgt gaggaagatc ttcaggtcga aaaatccgcg actggccggg
     7021 agcattccgg gattcatttt ccgtttactg gaaaagatta tccaccagga cgggatcaac
     7081 gattttatgt accggaacag ggataaatgg ggattggatt atgctgaagc aattctggat
     7141 ggattcaggg tttcaacccg cgttgtcaat cctcctgatc tgagccacgg aagatacctt
     7201 attgcttcca accatccgct cggtggaatg gatgggattg cactgatcca tgaaaccggt
     7261 aaattaaaaa aagacctgct ttttccggta aatgaccttt tgctcaatct gccgaacctt
     7321 tatgaattgt ttataccggt gaacaagcac ggcagcaatg ccgaaaatat caggctgttc
     7381 aatgatactt ttgcttctga cgttctgatt ttgtattttc cggcaggcct ggtttcaagg
     7441 aagcaagccg gagaaattaa agatgtggaa tggaaaaaaa ctttcctgac aaaagcccgg
     7501 cagtcaggaa gagatattgt tcctgttcat atggccggta gaaataccga attcttttat
     7561 aacctggcta atttcaggaa aagactgggg ataaaggcga acattgaaat gctgttttta
     7621 cccgatgaat tgtttaaata ccagggcaag gaaatagtta taacttatgg aaatccaatc
     7681 ccgatcggta cttttaacag gagcaaatcc gaccaggagt gggcttcatt gctccgtgag
     7741 catgtttata aactggaaag gaatcccaat gagattttta actattgatt tttaacaaaa
     7801 taggattatt atgcaggcag tcattccacc cgttaacaag gctctgctgg aagccgaact
     7861 aaccccggaa aagtttgtca ggaagacgaa taacggtaat aacctgattt acatttttga
     7921 ttacgacagt tgcccgaacc tgatgaggga gattggaagg ctccgggaga tcaccttcag
     7981 ggattcaggt ggaggaacag gaaaagataa tgatatcgat gaatttgacc tgggcgacaa
     8041 gccattccat cagttaatcg tatggaaccc ggaagataag gagatcgttg gcggataccg
     8101 gtttgcccag ggcgacaggt tgaagatcaa tgataaaggg gaagtgcaat ctccaactgc
     8161 tgaattattt gagttttctg ataaatttgt taaagattat cttccctggg gtatcgaact
     8221 cggccgctca ttcgtccagc cgaattacca gccgcagttt aaccttagaa aagggatgta
     8281 ttctctcgac aatatctggg atgggcttgg cgctatcgtg atagataatc ccgggtataa
     8341 atatttcttc ggcaaattga ccatgtaccc tcattatgaa tactttgccc gtgatatggt
     8401 cttgtatttt ctgcaaaaat tctttcccga tccggaagga ctgatgactc caagggagcc
     8461 tttacacctt cggcatgata aagattacct cgatagtttt tttaccggga gtaattatgt
     8521 ggagaactat aagatccttg tccagcaggt cagatcacac aatgaaaccg ttccacccct
     8581 ggtgaatgct tacatgagcc tgtcggccac tatgaaaacc ttcggcactt ccctgaaccg
     8641 ccatttcgga gatgtggaag aaaccggcat tatcatcacc atcggcgata tttatgattt
     8701 taagatcgac aggcatattg ccacatataa aaagtaatgg ccactaatcc gcttagcata
     8761 tcagcccgct ttatcaccag cagccccgaa tatacaaagt gtccggcgcc ggcgatgcct
     8821 gaatatgcct tcatcggccg ttcaaacgtc ggcaaatcct ccctgatcaa tatgctgacc
     8881 ggcgtcaaga tgctggccaa gaccagctcc acaccgggca agacacaact gatcaaccac
     8941 tttctgatca atgaaagctg gtacattgta gacctccccg ggtatggcta tgccagtacg
     9001 gctaaaatta agcgggaaca atggcccaat gtgattgaaa aatacctgct ttatcgaacc
     9061 agtcttatga cgacctttct attaatcgat tcccggctgg aattgcagga aaacgatcgg
     9121 aaagtaatta actggttcgg agagaacgga ttgccttttg tcatcgtatt gaccaaaacc
     9181 gacaagctta cccccaacca ggtgaatgca agccagctaa gactggcaaa taaactgaaa
     9241 gaagagtggg aagaattgcc tgtatttatc ctgacttcgt caaaaagcct ggaaggccgt
     9301 acggaaatcc tgcaatatat cgaccagact aatctgttat ttcaggtacc gtaaatatta
     9361 tgtcccggtt atctgaattc tgccagcaaa gtgcctttag caacggaaag cccgggtgta
     9421 acataaactt cttttatgac gcccttaagc ggtgaaaaaa tattattgtt cattttcatc
     9481 gcttccagta ccaggagaat ttccccttcc tttaccttgc tccctttttt gatatgtact
     9541 ttcctgattt ttcccgggat aaaggcgaag atctttttcg ggtcttcggg ttcatagggt
     9601 tttcggttgc ggaatttctc ggttattatt gtctggtaag taaaatcatc cagaagcatg
     9661 gaaccgtact gaggttgctg agaattctca tccgcttgtt tgtgtttatg ttcagtcatg
     9721 cttgtcggtt tagaaaggtg gtataccgtg ttttttcttt ggcggagtct cctgtttctg
     9781 catcgagatt tgcaaggcgt gcaccagcat attccgtgtt tcggatggtt caatgacggc
     9841 atcaatgtaa ccatacgccg cagcaacata tggattggca aacttctcct tgtactcctt
     9901 gatcttcagt ttacgggtcg cttccggatt atcggagttt ttgatctcgt tcctgaaaat
     9961 gatatttgcc gccccttccg ggcccatgac ggcaatttcg gccgttggcc acgcaaacac
    10021 aaagtcggct ttaagatggt gggagcacat ggcgatatat cctcctccgt atgcctttct
    10081 gagtattatc gtgattttgg gtacggtggc ttccgaatag gcatacagca actttgcacc
    10141 atgccggatg acgcccgcat gctcctggtc tattccgggc aaatatcccg ggaggtcaac
    10201 cagagtaagc aaaggaatat taaaggcgtc acagtaccgg ataaaccggg ccgctttatc
    10261 cgaagagtca acatcaagca ccccggcgag gtataacggc tggttggcca cgcatccgac
    10321 cgtttcgcca tccacacgtg caaatccgat aacgatattc ctggcccaaa gttcctggat
    10381 ctcaaggaac tccgaatcat ccgccagggc tttgatcaca tcgcggacat catacggttg
    10441 ctttggatcg gaagggatga tctggtcgat tttgaagctc cggctcttgg gcggcctttt
    10501 ggggaaggaa tcggcccgtt tctggttatt ccatggaata aaggtgatga gttgcttgat
    10561 ctggtcgaaa cactcctgtt cgctctcggc aaaaaagtgg gcattacctg tcttttccgc
    10621 atggaccctg gcaccgccga gatcctccat cgatatttct tctccaatcg tggctttgat
    10681 gacttccggg ccggtgatga acatcttgga tatcttatcc acgacaaaga caaaatcggt
    10741 cagggccggc gaatataccg cccctccggc acatggcccg agaatgactg aaatctgcgg
    10801 gatcacgcct gaagcttgtg tgttccggta aaatatttct ccatatccgg ccagcgaatt
    10861 caccccttcc tggattcggg ctccgcctga atcgttgatg ccgatcagcg gcactttcag
    10921 cttcatggca tggtccatga tcttggtgat cttttttgca tgcatgtaac ccagcgaacc
    10981 gcctgctacg gtaaagtcct gcgcaaaaat
//
LOCUS       DAOPNH010000086        10998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6371_length_10998_cov_6.913369, whole genome shotgun sequence.
ACCESSION   DAOPNH010000086 DAOPNH010000000
VERSION     DAOPNH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6371_length_10998_cov_6.913369"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..601
                     /locus_tag="PKZ74_06060"
     CDS             194..601
                     /locus_tag="PKZ74_06060"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM92357.1"
                     /translation="MPQRPRSHNPHVPGYNARFVILLDMVGAGNARFLKEGFSMHFAP
                     DKVKKVWDIAASLGYQEYFRDEKGAFINDDHYFINEIRKIPAIDIIHLDPNSANGSFY
                     DYWHTTQDTFDKIDKRTLQVVGDVVTKVVYTEK"
     gene            647..2926
                     /locus_tag="PKZ74_06065"
     CDS             647..2926
                     /locus_tag="PKZ74_06065"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM92358.1"
                     /translation="MKKFPAVLCIFFPTLWFTNTLTAQPEFPPNAPVFDSSTVARIDI
                     TIDPDTLAWIYENVDSDIEFHATFRFENQTIDELVENVGFRLRGNTSRYSQKKSFKVS
                     FNTFEPGKKFQGMEKLNLNGEHNDPSIIRSRICWEWLRTAGVPAPRAAHATVYINGDY
                     YGLYIMVEHVDEEFAESRFGNKNGNLYKCLWPADLDYLGNDPDLYKFYQNGRQAYELK
                     INEEYNNYSDLAHFIDVLNNTPPEDLECELEKVFNLNDYLKIMALDVITGNWDGYIYN
                     KNNFYLYHNTETGRFEYIPYDLDNTLGIDWMDRDWATRDIYDWEQHGTEVRPLYARLL
                     EVPGIKDRYSFFFKKFLDLLSEEDSLITAIGGIRTMIAPFVMNDPFYPLDYGYTYDNF
                     INSYDQALGGHVDYGLIPYIQTRRTTALQQLVINGIPPVINYIESSKAVPGEEYWIRA
                     TVTDESADFTVKLKYSINGGTELLVNMKDDGTHHDHEEDDGCYGYEFDGFDIGQILSW
                     QVMVTDPESMITIRPCSPVMIDFHSSDDPLIYINELMADNDTTIEDEFGEYDNWVEIY
                     NADDHPVWMGDKYLSDNLSNPDKWKLPDVSIPPGGFLLIWTDGQPGQGPFHTDYKLND
                     EGESLAIFDNESTGYFLIDSISWGLIPIDVSYGREEDGFLSWIYFNHATPGYSNGSFG
                     TDEPEPVVKSTRFYPNPVKNGTIYFDDIFNGCILNATGMIVMRIEGEKITNVGHLRSG
                     LYFLLDSETGRSFKVFIIH"
     gene            complement(3008..3928)
                     /locus_tag="PKZ74_06070"
     CDS             complement(3008..3928)
                     /locus_tag="PKZ74_06070"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPM92359.1"
                     /translation="MKKLLTILLSATVLFAFAEERIANNPKPINHGPLACPSLVMTPV
                     DNTVTYPSDLIEAILGPDVVSYSLINFAGLAGNPYASAGLFVGGTDAGLGIEDGIVMC
                     SGYITNAIGPNSDDGASVSLGLPGDAQLSAFAGQSTFDATVLEFSFVPNFDQLYIQYV
                     FGSEEYNEYVFQYNDAFAFFLNGNNIAVVPSTTLPVTINTINLSTNNTYYKNNDYGDL
                     YPGPYPYCNEMDGFTTVLVAQGPVNEGESNTIKLAIADGGDSALDSFCFIKGSSFGGT
                     DPEVPVSNWALFIGIGLILIFAVIRFRRLV"
     gene            complement(4073..5173)
                     /locus_tag="PKZ74_06075"
     CDS             complement(4073..5173)
                     /locus_tag="PKZ74_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92360.1"
                     /translation="MSHKQDYWIIRMTDTVKIAGKLIRAGTLLGLVLLYCITMAAGMG
                     LSSCPAIDGENELIIYVIPSKVKYDWNSPNSLVKSYIKNIRRNIFDKKSYLLGHAFVE
                     LNTSASPEGILTGMRSARPEEAKNLVIGEYYGLAILGADISGELESREELEKKIEFYS
                     GNGRLAFIKLIINDKAVERMLTFFEGYKAFWEQDSTTGARYGGAFWPRNKGEGAGCSA
                     FVVSFLDLGGLLREAFDDWKVEIDIPMKLIGGPYNEGNKVRIRDIRREQKWADRQSET
                     NNAFEHFEIYDPTLMYEWIHRLWESSDIQEKYAAVPVELNEAKGIRIDARQIQPDENE
                     PVFYHRQTPSIFIDYYKTRFPAWYNLTLPACK"
     gene            5208..6992
                     /locus_tag="PKZ74_06080"
     CDS             5208..6992
                     /locus_tag="PKZ74_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPM92361.1"
                     /translation="MSRSTKRAIWFRFMVWQARRMNHRTFLMIISVVIGILSGISAII
                     LKNAVHFIQELLISSAPIYRENFLYFALPAIGILLAIFFVKYVIKDHVEHGVPSILYA
                     ISRSNGIIRRHNMFSSIVTSALTVGFGGSVGLEGPTLATGAAVGSSLGQYLRLSYKDL
                     ILMMTLACAGAMAAIFKSPIAAIVFAVEVLMIDLTMASLIPLLIASVTALLTSYFFLG
                     NAVLYPFEVTEAFYYKQIPYYLILGVFTGFISVYFTRMYIRIEKYFKRISSWWLRWIS
                     GGMILGMLIFFFPALYGEGYEAVNTCMKGDYSPLFNGSPFYEFQDSIWVVFLLFILVL
                     FFKVVATATTFGAGGVGGIFAPSLFMGVIAGLFFAEMVNYSGLTLLSGSNFAMVGMSG
                     VIAGVLHAPLTGIFLIAEITGGYDLIIPLMLVSTISYATTRMFVSNSVYTYQLAARGE
                     LITHDKDKTAMSLLRVNDLIETDFRTIGPDARLRNLIQVISESARNIYPVVDENNRFF
                     GFVRLDDVRHIMFKQELYDQVIVKDIMVRPDTFVSPDDSMETVVEKFAHYDKYNLPVL
                     LNGIYIGFVSRANVFRNYREIVRKFSED"
     gene            complement(7051..9099)
                     /locus_tag="PKZ74_06085"
     CDS             complement(7051..9099)
                     /locus_tag="PKZ74_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPM92362.1"
                     /translation="MMPDHRFTNQLIRESSPYLLQHAHNPVEWHPWNEQTLSKARSLD
                     KMLLISIGYSSCHWCHVMERESFEDVKVAEIMNRNFICIKVDREERPDVDQVYMNAVQ
                     LITGSGGWPLNCFTLPDGRPFFGGTYFRKNQWVQLLENISTLYLKRRKEIEDQADALT
                     EGIRKASLIKPAESVRAITREDITRITNSLTGQMDELEGGFGGAPKFPMPAALAFLLT
                     TGEITANEKVEKLVKLTLRKMSYGGIYDQAGGGFSRYSTDSQWKVPHFEKMLYDNAQL
                     ASLYLEASQVYGSKEFRETAEETLGFVMREMTSPEGGFYSALDADSEGKEGRFYTWTA
                     GELKILTGNQGDLVRKYYHTGGKALWEEGQNILVRTQSPDEFARAQGLQPSAFKAILK
                     SAKAKLLKERNRRTMPGLDNKILASWNGLMMKAFADAGRITGQKKYLDAAVKTAGFIH
                     ANMTGPDGHIFRCFNKGKSYINGFLEDYCFVAEGLLALYLATFREQYLYRALELIETA
                     IRDFYDSESSYFFGSSASDAPLVARQQEIYDSVIPSSNSVMAGLLYKLGVIFEKDDFI
                     EKQAKMVSGVREKALQFPSAFANWASVMLQTALPYYFVVITGDDYPEKIATIRKHFRS
                     QIFFCAARERSELPVFKDRFTENQTMIYICTGRECLLPTTSVDEALQQLAGKIRLTPE
                     "
     gene            complement(9148..10185)
                     /locus_tag="PKZ74_06090"
     CDS             complement(9148..10185)
                     /locus_tag="PKZ74_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM92363.1"
                     /translation="MSGNLVTSEIIEQVARKAGITKKLASELLHAIPEIIAEGLQRDG
                     EVRVRGLGTFRMKWTQTRIGRNPKTGEQVPIPPHNRLVFLPEQAFKDFINRDLVALEY
                     QVIPEDASVPEPEKPAVEIPEPIIISPSWPEPLPEPEPYAIAGTGIDQEPGSTGSRRR
                     LHWIIPAAIAVIAILTVVFYFRIFREKEPVAVGSQQSAVGSQQPAVGSQQSADSSRQP
                     AVGSQQVEESNNDSTLIIKNSELSIKNSTLITSGVHLFQVSRDVYEGNPYLWALIYKA
                     NREIITNPQNLPSGLTLIIPGLEGSPNKLSRRDSSEVSEGYRLLSEYYREKDPSLSDG
                     FLKTSSQFRPE"
     gene            complement(10182..10469)
                     /locus_tag="PKZ74_06095"
     CDS             complement(10182..10469)
                     /locus_tag="PKZ74_06095"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM92364.1"
                     /translation="MNTKEFIQVLAEKLAITQKEASGLLEHTTAVMREAVAADQKVTI
                     THLGSFQLKKTASRAAYLPALDKKAIVPPKNSVQFHAADSLKDNLKKSPEK"
     gene            10683..>10998
                     /locus_tag="PKZ74_06100"
     CDS             10683..>10998
                     /locus_tag="PKZ74_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92365.1"
                     /translation="MKIFAPPYGILTLILLALSCMFSMTVKAQSIQIDSTFITDAEIF
                     PFENIELISELLIEGDVELNRDTSLVRVILEDENGFRYMVLEAYPLICPEIDFTVQDH
                     CDE"
BASE COUNT         2651 a         2658 c         2661 g         3028 t
ORIGIN      
        1 cgcgccagtt cgacgacatc cgccgccacc tcgcggccct ccggcgtctc gagccgaagc
       61 ctggacgaat cggcgaggaa gccccgcggc agcggcaggc cgaagccgat cgcctgagcg
      121 tcacttccgc ctgccgcgca gtggacggtc gcgtctacct ttccggtggc ctgcggcgcg
      181 tcgtaaggct cgggtgccgc aacggccgcg gtcgcataac ccgcatgtgc cggggtataa
      241 cgcccgtttt gtgatcctcc tcgatatggt gggagccgga aatgcccgct tcctgaaaga
      301 gggcttctcc atgcactttg cccccgataa ggtgaagaaa gtgtgggata tagccgccag
      361 ccttggatac caggaatatt tccgcgatga aaaaggggct tttattaacg atgatcacta
      421 ttttatcaat gagatccgca aaatccccgc catcgacatt atccaccttg atcccaactc
      481 cgctaacggt agtttttacg attactggca tacaacacaa gacactttcg ataagattga
      541 taaaagaact ttacaggtcg tcggggatgt ggtgaccaaa gtagtttata ccgaaaagta
      601 gggttggcct gcatttctgt tattgaagga ttaaaatcgt tttatgatga aaaaatttcc
      661 agcggtactc tgcatcttct ttccgactct gtggtttacc aacacgctga ccgcccaacc
      721 cgagtttccc cctaacgctc ccgttttcga cagttcaacc gtagcacgga tcgatattac
      781 cattgatccc gatacgcttg cctggatcta tgaaaatgtg gattcagata tcgaatttca
      841 tgctacattc cgttttgaaa atcagacgat tgatgaattg gttgaaaatg taggattcag
      901 gcttagggga aatacttcgc ggtattcgca gaagaaatca tttaaggttt cattcaacac
      961 attcgaaccg ggtaaaaagt tccagggaat ggagaaactg aacctgaacg gtgaacataa
     1021 cgatccttcc atcatacgtt cgcgcatttg ctgggagtgg cttcgtacag cgggtgtccc
     1081 ggctccccgg gccgctcatg ccacggttta tatcaacgga gattactacg ggctttatat
     1141 catggtcgaa catgtggatg aagaattcgc ggagtcgcga ttcggcaata aaaacgggaa
     1201 cctgtataaa tgcctctggc cggccgacct cgattacctc ggcaatgatc cggatctgta
     1261 caaattttat caaaacggcc ggcaggctta tgaattgaag atcaatgaag aatataacaa
     1321 ttactccgat cttgctcact ttattgatgt tttgaacaat accccgccgg aagatctgga
     1381 atgcgaactg gaaaaggtct ttaatctcaa cgactacctg aagatcatgg ccctggatgt
     1441 gatcactggc aactgggacg ggtacatata caacaagaat aacttctacc tttaccataa
     1501 tacagaaacc ggcaggttcg agtatattcc ctacgatctc gataatacgc tgggaattga
     1561 ttggatggat agggactggg cgactcgcga catctacgac tgggagcaac atggcacgga
     1621 ggtaaggccg ttgtatgcac ggcttctgga ggtacccggg atcaaagacc ggtattcttt
     1681 cttctttaaa aaattcctcg atcttttatc tgaagaagat tctttgatta cggccatcgg
     1741 gggaatacgg acaatgatcg ccccttttgt gatgaatgat ccgttttatc cgctcgatta
     1801 tggttatacg tatgataact tcataaattc atacgaccag gcgctgggcg gtcatgtcga
     1861 ttacgggttg atcccttata ttcagacccg gcggacgaca gccttgcagc aattggttat
     1921 caatggcatt cctcctgtta tcaactatat cgaatcatca aaagcggtac ccggtgaaga
     1981 atactggatc agggcgacgg ttaccgatga gtcggccgat tttacagtga agctgaaata
     2041 cagtattaac ggggggactg aattactggt gaatatgaaa gatgacggaa cacaccatga
     2101 ccatgaggaa gacgacggat gttatggcta tgaatttgac ggtttcgaca ttggacagat
     2161 cctttcatgg caggtgatgg tcaccgatcc cgaatcgatg atcacgatcc gcccgtgttc
     2221 tccggtgatg attgattttc attcttcgga tgatccgctg atctacatta acgaattgat
     2281 ggccgataac gatacgacca ttgaggatga attcggtgaa tatgataact gggtcgagat
     2341 ttataatgcc gatgatcatc cggtttggat gggggataaa tatttgtcgg ataatctttc
     2401 caatcccgat aagtggaaac tgccagatgt atcaatacct ccaggtggat ttctgttgat
     2461 ctggaccgac ggccagccgg gacaggggcc atttcacacc gattataaac tgaacgatga
     2521 aggtgagtcg ctggccatct tcgacaatga atcaacagga tattttttaa ttgacagtat
     2581 ttcctgggga ttgatcccga tcgatgtatc ttatggcaga gaagaagacg gatttctttc
     2641 ctggatttat tttaaccatg ccacgccggg atacagcaac ggaagctttg gaacagatga
     2701 acctgagccg gttgtaaaaa gcactcgatt ttatccgaat ccggttaaaa atgggactat
     2761 ttattttgat gatattttca atggctgcat tttaaacgcg acaggcatga ttgttatgcg
     2821 gattgaagga gagaagatca ccaatgttgg acatttgcgg tctggattgt atttcctgtt
     2881 agattcagag acagggagat ccttcaaggt ttttattatt cactgatgtt ccattttgcg
     2941 ttttgcaaac aaaaaggctg cctggaaaac ccggcagcct ttttcattta aagattctat
     3001 tattgtacta aaccagtctc ctgaaacgga tgacggcaaa aattaagatc aggccgatac
     3061 caatgaataa agcccagttg ctgaccggca cttccgggtc ggttccgccg aagctcgatc
     3121 ctttgataaa gcagaatgaa tcgagagctg agtcgccacc gtcggcaatc gccagcttaa
     3181 tggtattgga ttctccttcg ttcacgggtc cttgtgcaac cagaacagta gtaaatccgt
     3241 ccatctcatt gcaataagga tacggaccgg gatataaatc gccatagtca ttattcttgt
     3301 aatacgtgtt attggtagac aggttgatcg tattgatcgt taccgggagg gttgtgctcg
     3361 ggacgacggc gatgttgttg ccattgagga aaaatgcaaa agcgtcgttg tactggaaaa
     3421 catattcatt gtactcttcc gaaccgaaaa catactggat gtaaagctgg tcgaaattcg
     3481 gcacaaagct gaattcgagg acagtggcat cgaaggttga ttgtccggca aatgcggata
     3541 gctgggcatc tccgggaaga ccaagactaa ccgatgctcc atcatcagaa ttgggtccga
     3601 tagcattggt aatatatccg gagcacatca cgattccgtc ttcgatccct aatccggcat
     3661 cggttccgcc tacgaataaa ccggcggatg cataaggatt accggctaac ccggcgaagt
     3721 tgatgagcga ataagagacg acatccggtc ctaagatcgc ttcgatcagg tcgctgggat
     3781 aggtaacggt attgtcaacc ggggtcatga ccaggctggg gcaagccagc gggccgtgat
     3841 tgatcggttt cggattattg gcaatacgct cttctgcaaa agcaaaaagc accgtagcac
     3901 tgagtagaat tgtcaaaagt tttttcataa tgattgtttt tgaggttatt tgttattttc
     3961 tattgttcta ttgttctatt gcccaactga aattagaatg gcaaagataa actgcattac
     4021 ccgttatgtc aagaaaatga tgtacggatg aagtttattc gtacactttt tttcatttgc
     4081 acgccggaag cgtcaggtta taccatgccg ggaaccgtgt tttataataa tcgatgaaaa
     4141 tggaaggagt ctgccggtga tagaaaacag gctcattttc atcaggttgg atctgccttg
     4201 catcgatccg gatccctttt gcctcattca gttccaccgg taccgcagca tatttttcct
     4261 gaatgtcgct gctttcccag agccggtgaa tccattcgta catcagggtc ggatcataaa
     4321 tttcaaaatg ctcgaaagca ttattggttt cgctttgccg gtcagcccat ttttgttctc
     4381 tgcgtatatc cctgatcctg accttattgc cttcattata gggtcctccg atcagcttca
     4441 tcggtatgtc aatttctact ttccagtcat caaacgcctc ccggagcaaa ccacccaggt
     4501 ccagaaaact aacgacaaat gccgaacatc cggccccttc tcctttgtta cggggccaga
     4561 atgctcctcc atatctcgcc cctgttgtac tgtcctgttc ccagaacgct ttgtatcctt
     4621 cgaaaaaagt aagcatacgc tctactgctt tatcattgat aatgagttta ataaatgcca
     4681 gccggccatt ccccgaataa aattcgatct tcttttccag ttcttcacgg ctttcaagtt
     4741 ctcccgaaat gtcggctcca aggattgcta acccataata ctcaccgata accaggtttt
     4801 tagcctcttc cggcctggct gacctcatcc cggtcaatat cccttccgga ctggcggaag
     4861 tgtttaactc aacaaatgca tggcccagca gataactctt tttatcgaaa atattccgcc
     4921 tgatattttt tatataactt ttaaccaatg agttaggtga attccagtcg tattttacct
     4981 tcgatggaat gacgtaaatg atcagttcat tctcaccgtc aatggccgga caggacgaaa
     5041 ggcccatccc tgcagccatc gttatacaat aaaggaggac cagcccaagc aatgttccgg
     5101 ccctgatcag tttcccggct attttaaccg tatccgtcat ccttattatc caataatcct
     5161 gtttgtggct cacgaaatta aaaaatttag ataaattagc ccaatttatg tcaagatcca
     5221 ctaaaagggc tatctggttc aggttcatgg tctggcaggc caggcgaatg aaccatcgca
     5281 ccttcctgat gatcatcagt gtcgtaattg gcatattatc agggatctcc gccattattc
     5341 tgaagaacgc ggtccatttt atccaggaac ttctcatcag cagcgcgcca atttaccgcg
     5401 aaaatttcct ctattttgct ttgccggcga tagggatcct tctggccatc tttttcgtaa
     5461 aatatgtcat taaagatcat gtcgagcatg gcgttcccag cattctttat gccatcagcc
     5521 gttccaatgg catcatccgg cgccacaaca tgttctcctc catcgtcacc agcgcactga
     5581 ccgtcggttt cggcgggtcg gtgggattgg aaggcccgac actggctacc ggcgctgcag
     5641 taggctccag cctgggccag tacctccggc ttagttacaa agacctgatc ctgatgatga
     5701 cgctggcctg cgccggcgct atggcagcca tctttaagtc gcctattgcg gccatcgtct
     5761 ttgccgtgga agtcctgatg atcgacctga ccatggcttc cctcatccct ctgctgatcg
     5821 cttcagtcac cgccctgctt acttcatact tttttcttgg caatgccgtt ctttaccctt
     5881 tcgaagttac cgaggccttt tattacaaac aaatacccta ttatttaatc ctgggtgtct
     5941 ttaccggttt tatatcggtc tattttaccc ggatgtatat ccgtattgag aaatatttta
     6001 aaaggatcag cagctggtgg ctgagatgga tatcgggcgg catgatcctc gggatgctga
     6061 tctttttctt cccggcacta tacggtgaag gctatgaagc tgtaaacacc tgtatgaagg
     6121 gtgattacag tccattattt aacggaagcc ctttttatga gttccaggac agcatctggg
     6181 tagtgtttct tctctttatc ctggttctct tttttaaggt ggttgcaact gccactacat
     6241 tcggcgcggg gggagtcggg gggatattcg caccgtcgct gttcatgggg gttatagcag
     6301 ggcttttctt tgccgagatg gtcaattatt caggcctgac tctcctgtcg ggttccaatt
     6361 tcgccatggt agggatgagc ggagtgattg ccggcgtgct ccacgcgccg ctgacgggca
     6421 tcttcctgat cgcggagatc actgggggct acgacctgat catcccgctg atgctggtct
     6481 ctaccatcag ctatgctacc acccggatgt tcgtctccaa ttcggtctat acctaccagc
     6541 tcgctgccag gggagagctg atcacccatg ataaagacaa gacagccatg tcgctgctcc
     6601 gggtcaacga cctgatcgaa accgacttcc ggaccatcgg accggatgcc cgcctccgca
     6661 acctgatcca ggttatcagc gaatccgcca ggaacatcta cccggtagtc gatgaaaaca
     6721 acaggttttt cggattcgtc aggctggacg atgtccgtca tatcatgttc aaacaagaac
     6781 tctatgacca ggttattgtc aaagatatta tggtcaggcc ggatactttt gtctcacccg
     6841 atgattccat ggaaacggtc gtggagaaat ttgcgcatta cgataaatac aacctgccgg
     6901 tgcttttaaa tggcatttat atcggttttg tctcccgtgc caatgttttc cggaattacc
     6961 gggaaatcgt caggaaattt tccgaagatt gaagccgtca gttccctcct tagtcgggat
     7021 gacgcattct ctaagaaaat aaagggagcg ttattccggc gttagccgaa ttttacccgc
     7081 taactgctgc agtgcttcat caaccgatgt tgttggcagg aggcactcac ggccggtgca
     7141 tatatagatc attgtctgat tttcagtgaa cctgtctttg aaaaccggaa gttcactcct
     7201 ttcccttgct gcgcaaaaaa atatttgaga ccggaaatgc tttcggatag tcgcgatctt
     7261 ttcgggataa tcatcgccgg ttatgacaac gaaataatat ggaagggccg tctgtagcat
     7321 taccgatgcc cagttggcaa aggcggaagg gaattgcagg gctttttccc tgacacccga
     7381 aaccattttt gcctgttttt caataaaatc atctttttca aaaataacgc ccagcttata
     7441 aagcaaaccc gccatcacgc tgttggagga ggggatcacg gagtcgtaaa tttcctgctg
     7501 gcgagctacc agcggagcat cagaagcgga tgatccgaaa aaataactgc tttccgaatc
     7561 gtagaaatcc cggatcgccg tttcaatcaa ttccagagcc cggtacaaat attgttccct
     7621 gaaggtggcc aggtaaaggg ccaggagccc ttccgccaca aaacagtaat cttccaggaa
     7681 tccgttgata taagatttac ctttattgaa acagcgaaaa atatgaccat cggggccagt
     7741 catatttgca tgaataaacc cggcagtttt aaccgccgca tccaggtatt ttttctgtcc
     7801 ggtaattcgt ccggcatcgg cgaatgcctt catcatcagt ccgttccagg aggccagaat
     7861 cttgttgtca agccctggca ttgtccttct attccgttct ttaagcagtt ttgctttggc
     7921 gctcttcagg atggctttaa atgccgatgg ctgcaaaccc tgggccctgg caaattcatc
     7981 gggtgactgg gtgcgcacca ggatattttg cccctcttcc cacaaagctt tacctccggt
     8041 atgataatat tttcgcacca ggtcgccctg gttaccggtg agaatcttaa gctcaccggc
     8101 tgtccaggta tagaaccggc cttctttgcc ttccgaatcg gcatctaaag ccgaataaaa
     8161 gccgccttcg ggcgaggtca tttccctcat gacaaatccc agtgtctcct cggccgtttc
     8221 ccggaattcc tttgatccat atacctgtga ggcttccagg tagagagatg caagctgtgc
     8281 attgtcgtac agcatcttct cgaaatgggg gaccttccat tgggaatcgg tcgaataccg
     8341 ggagaatccg cctccggcct ggtcatagat tccgccgtat gacatcttcc gtaaggtcag
     8401 cttcaccagt ttttcgacct tctcattcgc agttatttcg cctgtggtta gtaagaacgc
     8461 caaggcagct ggcatcggaa atttcggggc gccgccgaat cccccctcca gttcgtccat
     8521 ctggccggtc aggctgttgg tgatccgggt aatgtcttcc ctggtaatcg ccctgaccga
     8581 ctctgccggt tttatcaagg aggccttcct gatcccttcg gtgagtgcat cggcttgatc
     8641 ctcaatctct tttcttcttt taagatagag tgtcgaaata ttctccagga gctggaccca
     8701 ttggtttttt cgaaaatagg tgccgccgaa aaagggacgg ccgtcgggca gggtaaagca
     8761 gttcagcggc cagcccccgc tgcccgtaat aagctgcacg gcgttcatgt acacctggtc
     8821 gacgtcgggc cgctcttccc ggtcgacctt gatgcagatg aaattgcggt tcatgatctc
     8881 cgccactttc acatcttcga acgattctcg ctccataacg tgacaccagt ggcaggatga
     8941 atagccaatg ctgatcagca gcatcttgtc gagcgaccgg gcttttgaaa gagtctgctc
     9001 attccaggga tgccattcca cgggattatg cgcgtgctgc agcaggtagg ggctgctttc
     9061 acggataagc tggttggtaa accggtgatc gggcatcatg agataaagtt acgattttgg
     9121 ccggcggtat tcaggcagtt gataacttta ttccggccgg aattgtgaag aggttttcag
     9181 gaacccgtcc gacaaggagg gatctttttc ccggtaatat tctgatagta agcgatagcc
     9241 ttccgacacc tctgaggaat cgcggcggct cagtttgttg ggtgagcctt caaggccggg
     9301 tatgatgaga gtcagaccgg aggggaggtt ctgcgggttt gtgatgatct ccctgttggc
     9361 cttgtagatc agcgcccaga ggtacgggtt gccctcgtaa acatcccggg atacctggaa
     9421 aagatgaacc ccggaggtta ttaatgttga attcttaatg cttaattctg aattcttaat
     9481 gattaatgtt gagtcgttat tggattcctc tacctgctga ctgccgactg ccggctgccg
     9541 actgctgtct gccgactgct gactgccgac tgccggctgc tgactgccga ctgccgactg
     9601 ctgactgccg actgctaccg gctccttctc ccgaaagatc ctgaaataga aaacgaccgt
     9661 caggatggcg atcacggcga tggctgccgg tattatccag tgcaaccggc ggcggctgcc
     9721 cgttgacccg ggttcctggt cgattcctgt tccggcaata gcatagggtt caggttcggg
     9781 tagcggctca ggccacgatg gactgatgat gatcggctcc ggaatttcca cggccggttt
     9841 ctccggttcg gggactgaag catcttccgg gattacctgg tattccaggg ctacaagatc
     9901 cctgttgata aaatctttaa atgcctgttc cggcagaaac accagccggt tatgcggcgg
     9961 gataggaacc tgctcgcccg ttttcgggtt tcggccgatc cgggtttgtg tccatttcat
    10021 gcggaaagtg cccaaaccgc gcacccttac ctctccgtcg cgttgcaaac cttcggctat
    10081 gatttcgggg atggcatgaa gcagttccga agccagtttt ttggtgatcc ctgctttgcg
    10141 tgccacctgt tcgatgattt cgctggttac gaggttgccg ctcatttctc cggggatttt
    10201 ttgagattat ctttcagcga atcggcggca tggaactgca cgctgttttt tggagggacg
    10261 atggcttttt tatcgagcgc tggaaggtag gcggcgcggc tggctgtttt tttcagttga
    10321 aagcttccca ggtgggtgat cgtcactttc tgatcagcgg ccacagcttc gcgcattacc
    10381 gcagtggtgt gttcaagcag tccggaagct tctttctgtg tgatcgcgag cttctccgcc
    10441 agcacctgta tgaattcctt ggtgttcagg gttgatttct ttggattgtt tcaaagtgta
    10501 atattacgaa aaaagattag gttttgcaag tttgagatgg agtctcctga aaaacgagac
    10561 tccatctggg ctaatcgaga tggagtctca aaatcaatct ttatattgct ttttatatta
    10621 attcagcata tatttacatc agccgaattt ttatttaatt attagcaatt aattcactga
    10681 ttatgaaaat atttgccccc ccctacggaa tcctgacctt aattcttctg gcgcttagtt
    10741 gtatgttttc catgactgtt aaagcgcaat ccattcaaat tgacagcact tttattaccg
    10801 atgctgaaat tttccctttc gaaaacatcg aactgatcag tgaactgctg atagaaggag
    10861 atgttgagtt aaacagggat acaagcctgg tcagggtgat cctggaagat gagaacgggt
    10921 ttcggtacat ggtcctggag gcttacccgt taatttgccc tgaaatcgat tttaccgttc
    10981 aggatcattg tgatgaaa
//
LOCUS       DAOPNH010000087        10918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6442_length_10918_cov_6.929393, whole genome shotgun sequence.
ACCESSION   DAOPNH010000087 DAOPNH010000000
VERSION     DAOPNH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_10918_cov_6.929393"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKZ74_06105"
     CDS             <1..484
                     /locus_tag="PKZ74_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPM92366.1"
                     /translation="NQNPLQGSFIIEELTDLVEEAVMMEFDRLTERGGVLGAMETMYQ
                     RGKIQEESLYYETLKHTGGYPIIGVNTFLSSEGSPTITPGEVIRATPEEKEYQISMLD
                     QLHITFAEKSDKLLEGLKDRARKNENIFEGLMEVAKYCSIGQITRALFDVGGQYRRNM
                     "
     gene            604..1236
                     /locus_tag="PKZ74_06110"
     CDS             604..1236
                     /locus_tag="PKZ74_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92367.1"
                     /translation="MVYWLDEIERHESRKKGSGSSSARVQDKKFRIGQNYAKNKNVYD
                     GFMSKMVTLINRVNNLPMEYREEFGKINAQQKDSKLDNHLTIFSSSRRIEKTEFKSIL
                     NPFKTVHYKHVRVIYFNIAKLMDKVEVEIYEELLEKKHRDGKIVPEHENPHHSQKPHS
                     DKEKFHEIFYYDMDKLNDELTTKVLDWLAFREEVNKIPIVFEGEPRFKES"
     gene            complement(1278..3287)
                     /locus_tag="PKZ74_06115"
     CDS             complement(1278..3287)
                     /locus_tag="PKZ74_06115"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HPM92368.1"
                     /translation="MEYKKLWTPDPEKVKNSQMYRYMQFVNEYTGSKYKTYDELWQWS
                     VGHPAAFWGTFWHYSGLKYSQDFNEVVDDPKKMPGAKWFTGARLNFAENLLQRRDDHP
                     AIIFRGEDGTSRTLTFRQLYEESHRVAEGLKRLGVQKGDRVAAFMPNIPETVIAMLAA
                     ASIGAIRSSTSPDFGIKGVLDRFSQIEPKVIFSVDGYYYNGKHFDSQEKLRGILGELP
                     TIETVVMVNFTGKTDLAGIPHGMAWESLALPVDGEMHFEQLPFDHPLYIMYSSGTTGL
                     PKSIVHSAGGTLIQHLKELKLHCDVRPDDVVFYFTTCGWMMWNWFVSSLAVGATIVCF
                     DGNPFYPAPDTLIRLSAELKFTLFGTSAKYIASLQQAGVRPVMETVVGTLHATSQLPK
                     NATSLQRFPLGFPSLRAIASTGSPLADESFDFVYNEWQRDVQLSSISGGTDIISCFML
                     GNPMLPVYRGEIQCRGLGMDVDCLDEEGHPVRDRQGELVCKTAFPSMPVYFWNDPDGK
                     KYFNAYFDHYPGIWYHGDYMILSRHGGITMLGRSDATLNPGGVRIGTSEIYRVVENME
                     EIDDSVVVGQQHEGDERVILFVKMKPGFVLDEDLAKKIKSSIRSACSPRHVPAVILTA
                     PDIPYTINGKKVEVAVKKLIHGQEVKNSDALRNAECLEFFRTVGL"
     gene            complement(3300..3995)
                     /locus_tag="PKZ74_06120"
     CDS             complement(3300..3995)
                     /locus_tag="PKZ74_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92369.1"
                     /translation="MRKEIYLVRGKEGEDYRAFTDRIMQTGRDAVKALRPLALKITVT
                     EAAPPPFSVIPFRKDKIAVISVYRDNSVTEDFFHDVEGISGAFKVEEALPVSYRKEWP
                     DGNPTPGVCLLTLFKRKPGIDYDLFIHRWHNGHTPLSLKIHPLWNYNRNVVLQKMCDS
                     DIWYDGIVEEQTRTRETLTNPFKFFGSFPVIIGNMIAVYRDVSSFIHYKSIETYLTIE
                     YHILSNETPSQNP"
     gene            4221..5273
                     /locus_tag="PKZ74_06125"
     CDS             4221..5273
                     /locus_tag="PKZ74_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM92370.1"
                     /translation="MDRNNLNVLVTGGSGFLGQAIVRELLEEDSPVRAGKIRIFDLKP
                     PADNHLPAHVEYFRGDVRDLQSLQAACKDIGLVIHSAAVIDWGILSDEEVIDINTGGT
                     KNIISACKSGGIGHLIFTSSLDAVYSGKPLVHIDESHPYPEQHKTSYCRSKYLAEAAI
                     KEACGEGLKAVVLRPSDIYGEADPYHIGSLINMAKGGFYVRLGNGKAKCQHIYVGNIA
                     HAHLQAAAALMNGSDHITGEVYFMTDAPGSNFFRFFDQVVIGAGYKIRPKNFWIPRPV
                     AYAMGSMSEFFAFMVRPFKKYHPKFSRFAVVYTCTDYTFTSDKACKDWGFKPKYSHGE
                     ALERTIGYYRSVKSER"
     gene            complement(5446..6984)
                     /locus_tag="PKZ74_06130"
     CDS             complement(5446..6984)
                     /locus_tag="PKZ74_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92371.1"
                     /translation="MIRNLLLLFLLIFSGVLSKGQFTYFNNHYNNDNWSAGISIVETE
                     SGYVAFGVSGVESGQYIFKRILLTGIDHQGNQIWWKTYGEDFHNYYAGLNRGGIKTSD
                     GGFAVSGAIEDETRVVGLLMKFDETGDSLWSRTYGDTITPGYSFNGFYACKQLPDHGY
                     LLAGTVYISGDDDDILLVRTDSLGNQIWRKSYGALHFIESAYSLVQLPDGEFLIGYMS
                     QNINVFYTLHPGMLKIDNQGNEIWSKIYSGPYDDSGCAVTISQDGNYLLGSSFGYTST
                     HPVITTFKVWIIKTDTSGNIIWDKIYGDKTYEGQAWTINEMNDGNIIVSGSGGFEETP
                     GIEGWILKTESDGDSLWMRRYSYFPTYNNEQNDLAFTSDNGLVLTGMTMGYPDWIESV
                     WIQKLDSIGCDSVGCDTTVGIYEGHGGVEAGGHGGMEIYPNPASDLIHITFRQNSLNR
                     FPARDRELEIFNVYGEKVLGIKLSVFQESITQNISGLAQGLYLVVLRERQKVVATGKF
                     LIAR"
     gene            complement(7028..10843)
                     /locus_tag="PKZ74_06135"
     CDS             complement(7028..10843)
                     /locus_tag="PKZ74_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM92372.1"
                     /translation="MRDSLIRAKISEKVDKINEIIEEKRLPWYAGETPMALKTFQEKL
                     DYFGTTALPYLQGLEYYVGGFFVILPLEDDQNANRSYTITDEFDWRNRHGANDSSSLY
                     YDGDPTGSGWIPNRREGQYCYDCWAFAPVYTTEAIENLYYNEHIDENLAEQNIISCLN
                     DTLCWWGGNTTDAINFIINPGVMNEECFPYQPWHIQHCEDSCDNPNERISIYGKSYIH
                     GGQQNREYFLKKNLIVNGPQSTIMPIWKHAITMVGYGKIKAWDLILDGNNPWHEGDSE
                     KVILPGDPWIGSNYWIFKNSWGDWGANGTEFYYVIANAGQFRNYSLLTPLSSFLYDDS
                     DIRCADLDNDGYYNWGISLTKPNTCPTCSDSCDCDDSKSFLGPYDENYNCILLCDQFV
                     LDEDTLFISTDDTWYGKKYINQIVYIEDTVTLTIKGEVGFCEDATIIVGRGSELIIDA
                     GLLHGTCSNMWQGIQVWGTATAPQSTPGAQGKLTVINGGTIENAIAAVRMAKTDGIRY
                     EYGYEGGIIQTDDAHFINNRIGVRFYPYRYQVSGVEVDNLSYFYNSEFLTDAELADQS
                     LPEKFIHFDNIKGINVKGCSFINSRPEEETEMNERGVGIYSEDANFTVTDYCLDPPGC
                     EDLIPSVFQLLNYGVEAFKEETTKSFDVNNSNFYNNLTGIYIKGVDNPEIIFNNFELK
                     SVSLFHQPGDIFGGLYLDGAPRFTVEENYFYNEDSYDPQSEISSIGITVSNCGDLQNI
                     IYRNTFDRLHLAILAQGRNRGTSISTGLKLHCNVCTNNMNDFAVTADGSALNQGISER
                     QGAFRVGDVTAPAGNLFSHEFSTDPYRDFNNTLSGSRITYYQHTGSNGDPWELTYYQN
                     ITKNEYGTYTGHDDACPAHFYSGGHKKSAEIVPDEDDLKDLLIQREYQRDSLINILNL
                     WVDAGDTPQLDYTVDSSTPDEALEVYDELISYSPYLSDTVLASSIDKQDVLVNAMVRD
                     IMVANPHGAKKEELVESLEERIPPLPDYMMAEIMNGRDTLSLKELKEGEITWYEQERN
                     SAFAALLNLYLTQTTESSGDSLVSLISNHGYLKDHYFLAAGFLEKGDIANTFSVLDEL
                     PTKFSMTSGELSEYQDYLTLFSILGDLKLSGQPLDSMNASSRQTLYQIAEDNFAPGML
                     AQNILQRIDTAEYHEIYILPTSEPAENKYYGENPDLNANAEDGVFRVYPNPCLDYFII
                     EYFIKIVPQDVTYSIVDPLGRIIEEDKIVGQQNQIIRRASEFSPGVFTIQLILDGKVV
                     KAAKLTIIK"
BASE COUNT         3012 a         2540 c         2352 g         3014 t
ORIGIN      
        1 gaaccagaac ccgctccagg gatcgtttat catcgaggag ctgaccgacc tggtggaaga
       61 ggccgtgatg atggaattcg accggctgac ggagcgcgga ggggtgctgg gcgccatgga
      121 gaccatgtac cagcgcggca agatccagga ggagtcgctc tattacgaga cgctcaaaca
      181 taccggcggg tacccgatca tcggggtgaa cacgttcctc tcctccgaag gctctccgac
      241 catcacgccg ggcgaggtga tccgggccac accggaggaa aaagagtacc agatcagcat
      301 gttggaccaa ttgcatataa ctttcgcgga aaagtcggat aaattgctgg aagggctgaa
      361 agaccgcgcg aggaaaaatg aaaatatctt tgaaggtttg atggaggtgg cgaaatactg
      421 ctccatcggg cagatcacca gagcgctctt tgatgtggga ggacaatata gaagaaatat
      481 gtaacaataa ttttgaattc ttaatgttta attcttaatt cttaattaaa ttgcttaatt
      541 ctactgtaaa atcagcatta ataattaaaa attaagaatt tagaattcaa cattctttag
      601 gtgatggttt attggttaga tgaaatagaa cggcacgaga gccggaaaaa aggatcgggt
      661 tcatccagcg cgagggtgca ggataagaaa ttccggatcg ggcagaatta tgcgaagaac
      721 aagaatgttt acgacggttt tatgagcaag atggtgaccc tgatcaaccg ggtaaacaac
      781 ctgcctatgg aataccggga ggaattcggc aagatcaatg cgcaacagaa agattccaaa
      841 ctcgataatc acctgaccat tttctccagt agccgcagaa ttgagaaaac cgaattcaaa
      901 agcattctga atccatttaa aacagttcac tacaaacatg tcagggtgat ttattttaac
      961 atcgccaagc tcatggataa ggttgaagtg gagatttacg aagagttact ggaaaagaag
     1021 caccgcgacg gcaagatcgt accggaacat gaaaatccgc accacagcca gaaacctcat
     1081 tcggataagg aaaaattcca tgagattttt tattacgaca tggataaact gaacgatgaa
     1141 ctgaccacaa aggtgctcga ctggctggcc ttccgcgagg aggtgaataa aatccctatt
     1201 gtcttcgaag gagaaccgcg gtttaaggag tcataagccg gtggtcggca gtcggcagtc
     1261 ggcgggtgaa gtatctccta cagtccaaca gtcctgaaaa actccaggca ttccgcattc
     1321 cgaagcgcgt cgctgttctt cacctcctgc ccgtggatca gttttttgac agctacttcc
     1381 actttcttgc cgttgatggt gtacggaata tcaggagcgg tcagtatcac cgccggcaca
     1441 tggcgcgggc tgcacgccga gcggatgctg cttttgatct tttttgcaag atcttcatcc
     1501 agcacaaatc cgggcttcat cttaacgaac aggattaccc gctcgtcgcc ttcgtgttgc
     1561 tgccctacca ccaccgaatc gtcgatctcc tccatgttct ccacaacccg gtagatctct
     1621 gaggtgccga tccgcacgcc cccggggttc agggtggcgt cggagcgccc cagcatcgtg
     1681 atcccgccat gccggctcag gatcatgtaa tcgccgtggt accagatccc cgggtagtga
     1741 tcgaaatagg cattgaagta cttttttccg tccgggtcgt tccagaaata gaccggcatc
     1801 gacgggaaag cggtcttgca caccagctcg ccctgcctgt cgcgcaccgg atggccctct
     1861 tcatctaaac aatccacgtc cattcctagc ccccggcact ggatctcgcc ccggtacacc
     1921 ggcagcatcg ggttgcccag catgaaacac gaaataatgt cggtcccacc cgaaatcgat
     1981 gacaattgca catcccgttg ccattcgttg taaacgaaat cgaacgattc gtcggccaac
     2041 ggggaacctg tcgacgcaat cgcccgcaat gatggaaacc ctaatggaaa ccgttgtagg
     2101 gacgttgcat tttttggtag ttgggacgtt gcatgcaacg tccctacaac ggtttccatt
     2161 accggtcgca cccccgcctg ttgcaacgac gcgatatatt ttgccgacgt tccgaacagg
     2221 gtgaatttca attcggccga caggcggatc agcgtatcgg gcgcagggta gaacgggtta
     2281 ccgtcgaaac agacgatggt ggcacctaca gccaggctgc tgacgaacca gttccacatc
     2341 atccatccgc aggtggtgaa atagaagacg acgtcgtcag ggcggacgtc gcagtgcagc
     2401 ttcagttcct tcaggtgctg gatcagtgtg ccgccggccg agtggacgat gctcttcggc
     2461 agcccggtgg tgccggatga gtacatgatg tacaacggat gatcgaaagg gagttgttcg
     2521 aagtgcattt cgccgtcgac cggaagggcc agcgactccc aggccatgcc atgggggatg
     2581 cccgccaggt cggtttttcc ggtgaagttg accatcacca ccgtttcgat cgtgggcagc
     2641 tcgcccagga tgccacgcag cttctcttgc gagtcgaaat gcttgccgtt gtagtaataa
     2701 ccgtcgacgg agaagatcac tttcggctcg atctggctga agcggtcgag aacgcctttg
     2761 atgccgaaat cgggggaggt tgacgacctg atcgccccga tcgaagcggc ggccagcata
     2821 gcgatgaccg tttcggggat attgggcatg aaggcggcta cccggtcgcc tttctgtacg
     2881 ccgagccgtt tcaatccttc cgccacccgg tgcgattctt cataaagctg ccggaaggtc
     2941 agtgtacggg aagtgccgtc ttctccgcgg aagataatgg ccgggtggtc gtcgcggcgc
     3001 tgaagcaggt tctctgcaaa attcagccgg gcgccggtga accatttcgc accgggcatc
     3061 tttttcggat cgtcgaccac ttcattgaaa tcttgagaat acttcaggcc gctgtagtgc
     3121 cagaacgtcc cccagaaggc tgccgggtga ccgacgctcc actgccagag ttcgtcgtaa
     3181 gttttatatt tactccccgt gtattcgttc acaaactgca tgtaacggta catctgtgag
     3241 tttttcacct tttccgggtc gggggtccag agttttttgt attccatagg gcggtttggt
     3301 tatggatttt gagatggagt ctcgttgctg aggatatggt attcgatggt aagatacgtt
     3361 tcaatacttt tgtaatgaat gaaggagctg acatcccggt aaacggcgat catattcccg
     3421 attatgaccg gaaaactgcc gaagaatttg aacggattgg tcagcgtttc tctggtccgg
     3481 gtctgctcct cgacaatacc gtcgtaccat atgtcgctgt cgcacatctt ctgcagcacc
     3541 acattacggt tataattcca cagcgggtgt attttcagcg ataggggagt gtgcccgtta
     3601 tgccaccggt ggatgaacag atcataatcg atgcccggtt tgcgtttgaa taaagtcaga
     3661 aggcacacac ccggcgtcgg attaccatcg ggccattctt tccggtaaga aaccggtaaa
     3721 gcttcctcca ctttaaaagc gccgctgatc ccttccacat catgaaaaaa atcttcggta
     3781 acactattgt ccctgtaaac cgaaatcacg gcaattttgt ctttccggaa agggatcact
     3841 gagaatgggg gcggcgctgc ttccgtcacc gttattttca gcgccagcgg ccggagggct
     3901 ttcacggcat cccttccggt ctgcatgatc ctgtcggtaa aggcccggta atcttctccc
     3961 tcttttcccc ggaccaggta gatctctttt ctcataatct ttgtttcttt gcaggcagaa
     4021 taaacagata gtaaagatag ttttttgttc tcacagactt tatgacattg attaatctta
     4081 ttaaggtagc cttgactaaa gatcagatcc gcggtaattc gtcttaattt gcgtcattcg
     4141 tgttctataa tggaacgcga ataaacgcga atacaacgaa tttgcacgga ttaatttatt
     4201 atttctaaaa gacaacataa atggacagaa ataatctgaa cgtgctagta accggaggct
     4261 ccgggttttt aggacaggcg atcgtcaggg agctgctgga ggaggactcg cctgtcagag
     4321 caggcaaaat caggatattt gatttaaagc caccggcaga taatcatttg ccggctcatg
     4381 tggagtattt ccggggggat gtccgcgacc tgcaatcgct tcaggccgcc tgcaaggata
     4441 tcggcctggt gatccattca gcggcggtga tcgactgggg gatcctatcg gatgaagagg
     4501 tcattgatat caataccggc gggacaaaga acattatctc tgcctgtaaa tccgggggga
     4561 tcgggcatct catctttacc agctcgctgg atgcggttta cagcggtaag ccgctggtgc
     4621 atatcgacga aagccacccg tatcccgaac agcacaagac ctcctactgc cgcagcaaat
     4681 acctggcgga ggctgcaata aaggaggcct gcggggaagg cttgaaggcg gtcgttcttc
     4741 gcccgtctga tatttatggc gaagccgacc cttaccatat cggttcgctg atcaacatgg
     4801 cgaaaggggg tttttacgtc aggctgggca acgggaaggc caaatgccag catatctatg
     4861 tcggcaatat tgcgcatgcc catctgcagg cggcggcggc gctcatgaac ggctcggacc
     4921 acattaccgg cgaggtctat tttatgaccg atgcgcccgg ttccaacttc ttccggttct
     4981 tcgaccaggt agtcatcggc gcaggttata aaatacggcc gaagaacttc tggatcccgc
     5041 ggccggtagc gtatgccatg ggcagtatgt cggaattctt cgctttcatg gttcgtccgt
     5101 ttaaaaaata tcaccctaaa ttcagccgct ttgccgtggt ctatacctgt accgattata
     5161 cgtttacttc ggataaagca tgtaaagact ggggattcaa gccgaaatac agccacgggg
     5221 aggcgctgga gaggacgatt gggtactata gaagcgtgaa gagtgaacgg tgaagggtga
     5281 agcgtgaaat tggaaaatag aaatttggaa ttggttcccg ctttcgcggg aaagacgcga
     5341 aacctggaac ctgaaacctg aaacctgaaa cctgaaacct ggaacctgaa acctggaacc
     5401 tgaaacctgg aacctgaaac ccggaaccct gggacctcac cgctcctatc tcgcaatcag
     5461 gaacttgcct gttgcaacga ctttttgtct ttccctgagc accactaaat acaagccttg
     5521 tgcaaggccg gaaatgtttt gggttatcga ttcctgaaag acggacaact taattcctaa
     5581 caccttttct ccgtaaacat taaagatttc caattctctg tctcgtgcgg gaaaccggtt
     5641 caggctattt tgcctgaagg tgatgtgaat cagatctgat gccgggtttg gataaatctc
     5701 catgcctcca tgcccccccg cctccacgcc tccatgccct tcataaatcc ctaccgttgt
     5761 atcgcaccca accgaatcac acccgatgct gtcaagtttt tgaatccaaa ctgactctat
     5821 ccaatccgga tatcccattg tcataccggt aagcacgagc ccattatctg aagtgaaggc
     5881 aagatcattt tgttcattat tataggttgg gaaataacta tatcgtctca tccaaaggct
     5941 atcgccatct gattcggttt ttaatatcca tccctcaatc cctggtgttt cttcaaagcc
     6001 tcccgaacct gatacaatga tattcccgtc attcatttca ttaatagtcc aggcttgacc
     6061 ttcatatgtt ttatcaccat agatcttgtc ccagataata ttcccggaag tatcagtttt
     6121 aataatccat actttaaatg ttgtaattac cgggtgtgtg gaagtatatc caaagctgct
     6181 tcctaaaaga taatttccat cctgggaaat tgtgacagcg cacccggaat catcatacgg
     6241 gccactgtat attttactcc agatttcatt tccctggttg tcaattttca gcattcccgg
     6301 atgcaatgtg taaaaaacat taatattttg gctcatatag cctattaaaa attcgccatc
     6361 aggtaattga acgagagaat atgcactttc aatgaaatgg agagctccat aactctttct
     6421 ccaaatttga ttaccaaggc tatcggtcct tactaaaagt atatcatcat catcaccgga
     6481 aatatataca gtgcctgcaa gcaaataccc atggtcaggt aattgtttac aggcatagaa
     6541 tccattaaat gaataccctg gtgtaatcgt atctccataa gttctcgacc acaaggaatc
     6601 accggtttca tcaaatttca tcaataatcc aacgactctg gtttcatctt caattgcgcc
     6661 actgactgca aatcccccgt ctgatgtttt aatgccgccc ctgtttaggc ctgcataata
     6721 attatgaaaa tcctctccat atgttttcca ccagatttgg ttaccctggt gatcgatgcc
     6781 ggtcaggagt attcttttga agatatattg gcctgattcc accccgctca ccccgaaggc
     6841 cacataacca ctttccgttt caacaattga gatgcccgcg ctccagttat cattgttgta
     6901 atggttattg aaataggtaa actggccctt tgaaagaaca ccggaaaaaa ttagtaaaaa
     6961 aagtaatagt aaatttctga tcatcattca gatatttgta gaaaaagatg aacttctatt
     7021 tattctgtta cttaattata gtcaatttag ctgcttttac aaccttccca tcaagaatta
     7081 attggatagt aaatacacca ggactaaatt ctgaagccct tctgatgatc tgattttgtt
     7141 gccccactat tttatcttct tcaataattc tgccgagtgg atcaactatt gaataagtaa
     7201 cgtcttgggg gacaatttta atgaaatact caataataaa ataatccaga catggatttg
     7261 gatagaccct aaaaactcca tcttcagcat ttgcgttcaa atccggattt tcaccataat
     7321 atttattttc agcgggttca gacgtcggaa gaatataaat ctcatgatat tccgcagtat
     7381 caatccgctg aagtatattt tgagctaaca tacccggtgc gaaattgtct tcggcaattt
     7441 gatatagtgt ttgacgagag gaagcattca tagaatctaa tggctgaccc gaaagtttaa
     7501 ggtcaccaag tattgaaaaa agagtcagat aatcttgata ctcggaaagt tcgccggatg
     7561 tcatagaaaa tttagttgga agttcatcca gtacagaaaa tgtattggct atatctcctt
     7621 tctctaaaaa cccggcagct aaaaagtaat gatcttttaa gtacccgtga ttacttataa
     7681 gggatactaa actatctccg cttgattctg ttgtttgcgt tagatataaa ttcaacaggg
     7741 ctgcaaatgc tgaattcctt tcttgttcat accaggtgat ttcaccttct ttcaattcct
     7801 ttaaagataa agtatcacgg ccattcatga tttctgccat catataatcc ggaagaggtg
     7861 gtattctttc ttcaagggac tcaaccaatt cttccttttt ggctccatga ggattagcaa
     7921 ccataatatc tcttaccatt gcgttaacta agacatcttg tttatcaata cttgaagcga
     7981 gcacagtatc ggaaagataa ggagaatatg aaattagttc atcatacact tcaagcgctt
     8041 catcaggtgt agaactatca acggtataat ccaattgagg tgtatctccg gcatcaaccc
     8101 aaagattgag gatatttatc agtgagtctc tttgatattc tctttgaatc aataaatcct
     8161 tcagatcatc ttcatctgga acaatttctg cagacttctt atgccctccg ctgtagaaat
     8221 gtgcgggaca tgcatcatca tgccctgtat aagttccata ttcatttttt gtaatgttct
     8281 gataatatgt caattcccac ggatcgccat ttgaccctgt atgttgataa taagtaatcc
     8341 ttgacccgga tagggtattg ttaaaatccc tgtaaggatc ggtactaaat tcgtgtgaga
     8401 ataaatttcc ggcgggtgca gtaacatctc ccacacgaaa tgctccctgg cgttctgaaa
     8461 tgccctgatt aagtgccgaa ccatcagcgg taacagcaaa gtcattcata ttatttgtgc
     8521 acacattgca atgtaatttt aaaccagtag aaatagatgt tccacggttt cgaccttgtg
     8581 ccaagattgc aagatggagc cgatcgaaag tgttccggta tataatattc tgaagatcac
     8641 cacaattgct gaccgtaatg ccaatgctgc taatttcact ttgtggatca tatgaatcct
     8701 cattatagaa atagttttcc tcaactgtaa accggggagc accatctaaa tacaagccac
     8761 cgaaaatatc accgggttga tggaagaggc tgacgctctt taattcaaaa ttattgaata
     8821 tgatttccgg attatcaaca cctttaatgt aaatccctgt aagattattg tagaaattcg
     8881 aattattgac atcaaatgat ttggtagttt cctctttaaa tgcttcaact ccataattca
     8941 agagttgaaa aacacttggg atcaaatctt cacaaccagg aggatccaaa caataatccg
     9001 taacagtaaa attagcatct tcactataaa ttcctactcc tctttcattc atttccgttt
     9061 cctcttccgg cctggaattt ataaaggaac agcctttaac attgataccc tttatattat
     9121 caaaatgaat aaatttctca ggtaaacttt gatcagccag ttcagcatcc gttaaaaatt
     9181 ccgaattata aaaatagctg aggttatcca cttcaacacc ggaaacttga tatctataag
     9241 gataaaacct gacgcctatc ctgttattta taaaatgtgc atcgtctgtt tgaataattc
     9301 cgccctcata accatattca tatctgatcc catcggtttt agccattctt actgctgcaa
     9361 tggcattttc gatagttccg ccgttaatga cggtgagttt tccttgcgcc ccgggggtgg
     9421 attggggagc ggtggctgta ccccaaacct ggatgccttg ccacatattt gaacatgtcc
     9481 catgaagtaa tccagcatca ataatcaatt ctgaaccgcg gccaacaatg atagttgcat
     9541 cttcacaaaa accaacttcg cctttaatgg tcaacgttac agtatcttca atataaacaa
     9601 tttgattgat gtatttcttg ccataccaag tatcatccgt tgaaatgaat aaagtatcct
     9661 cgtctaacac aaattggtca caaagaagta tgcaattata attttcatca tatgggccga
     9721 gaaatgactt tgaatcatca caatcacatg aatctgagca agtaggacag gtattcggct
     9781 tagtcagtga aataccccaa ttataatagc catcattatc taaatctgca catcttatat
     9841 ctgaatcatc ataaagaaat gaactgagtg gcgtgagcaa agaataatta cgaaactggc
     9901 cagcgttcgc aattacataa tagaattctg ttccattggc tccccagtct ccccaactat
     9961 tcttaaatat ccagtaattg ctaccaatcc aaggatctcc aggtaaaatt accttttctg
    10021 aatcaccttc atgccatgga ttatttccat ctaaaattaa atcccaagct ttaatttttc
    10081 catatcccac catcgtgatg gcgtgtttcc atataggcat aatagtgctt tgaggaccgt
    10141 ttacgatcaa attctttttt aaaaagtatt ctcgattttg ttgaccacca tgtatataac
    10201 ttttaccata aattgatatt ctttcatttg gattatcaca tgaatcctca cagtgttgta
    10261 tatgccaagg ttgatatgga aaacactctt cattcattac tccaggattt attataaaat
    10321 ttattgcatc agtggtattg cccccccacc aacatagtgt atcatttaaa catgaaatta
    10381 tattttgctc cgctaaattt tcgtcaatat gttcattata gtacaagttt tcaatagctt
    10441 ctgtagtata aactggggca aaagcccaac aatcgtaaca atactgaccc tcacgacgat
    10501 tagggatcca tccacttcct gttggatccc catcataata cagtgaactt gagtcattag
    10561 ctccatgacg atttctccaa tcaaattcgt ctgttatagt ataggatcta ttagcatttt
    10621 gatcatcttc taaaggtagt attacaaaaa aaccaccgac ataatattct aatccttgaa
    10681 ggtatggcag agccgtggtt ccaaaataat caagcttctc ttgaaaagtt ttcaatgcca
    10741 tgggtgtttc acctgcatac caggggagcc gcttttcttc aataatttca tttattttat
    10801 cgactttctc agatatcttt gctctaataa gactatctcg cacatattct aaattttcaa
    10861 aaccttgatc taaataggtt aaataatcaa catagatgga agcatcaatt aactgaat
//
LOCUS       DAOPNH010000088        10866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6497_length_10866_cov_6.558413, whole genome shotgun sequence.
ACCESSION   DAOPNH010000088 DAOPNH010000000
VERSION     DAOPNH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_10866_cov_6.558413"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PKZ74_06140"
     CDS             <1..1326
                     /locus_tag="PKZ74_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM92373.1"
                     /translation="PWSGRMLSDEGMILHFATPSYTAGTKGVQRGHVLIEPRTQAEFD
                     RMKGQLKGAWVLILGENDGWPIDISAHADSLRDSIKVTNAAIEEENNKIRRENRDNKE
                     KGLPEKELKELNEEPALFYREMREAGILGIIQSSKTPIRVMYDRKNIMDMTFDNLPAV
                     PDIKLDEHQYAVIKQMAEERRYFQLEFDIRNHFRPGPIPYYNVIGVIPGTEFPDEYVI
                     QGGHLDAYDVATGGVDDGSGATPSMEAARLIMAAGGKPKRTILTILWAGEEFGLLGSQ
                     SWVDRNMDQLGKISNIFNRDGGPTVITGISVPAAMREDFEQICAPINGINPDFPFKIE
                     ERQPSEMPKKAWGTDSGPFAVAGVPVLHFNTGDPKGYNFNYYEIWHTERDTYDKSIKE
                     YQDHVSIVTAIVVYGVANLDHLLNREGYWKAPEEEQGKGKKEKGKGGKE"
     gene            1397..2338
                     /locus_tag="PKZ74_06145"
     CDS             1397..2338
                     /locus_tag="PKZ74_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPM92374.1"
                     /translation="MKSVYRPVIALLILSIASYAAIGQGISVNKTGAPPDSSAILDIQ
                     STSQGVLFPRMSTWKIRNIQDPETGLMVFNTDSLELYIFTGQKWESVRDKTDTITTWE
                     CGQEFLYGGQAYSTVLIGTQCWMSENLNIGVRVEGTVEQHDNDTIEKYCYNNDPANCD
                     VYGGLYQWAEVVQYLNGATNFTSWDPAPEGPVRGICPEDWHIPSYGEWMELVVYCDYW
                     GGRLKETGTEHWFPPNTDANNESGFTAFPGGYRHPSNGGYAEFRYMGTLWSTTGYEPY
                     ETYSVYFIRLYFNNGGITWAIGIEKSWGFSVRCVMDN"
     gene            2407..4611
                     /locus_tag="PKZ74_06150"
     CDS             2407..4611
                     /locus_tag="PKZ74_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92375.1"
                     /translation="MHKILIIIGLIGICANIYCQEEAYNWYFGEQAGLSFAQGDPVAL
                     TDGALYTGEGCSAISSSSGELQLYSDGRFVYDRNHSIMPNGSGLLGHSSSTQSCIIVP
                     RPANNSQYYVFTVDAKDNGLVNGLCYSLVDMTLNGGLGDVSPAEKNISLLPYTCEKVT
                     AVRHNDGASVWVITHQWGSNAFYSFLVTAEGVNMTPVISHAGQAVSGDMEAAKGYIKV
                     SPDGARIAMANNTAFTVGIFSFDDASGVVSHIITDDSYVNPGGSDPGGPYGVEFSPES
                     DLLYISEWKTNRKISQYDLSSNDPQTILNSRVTVATVNQNADPFGAIQLAPDKRLYIA
                     RMGSNYMSAINFPDTAGPGCNFNDNAIELTGGNSRYGLPAFVQSYFNDIAAFTATPLK
                     DYAPLTVQFTDLSSGSPTGWKWDFENDGTFDAFIQNPSHTYDVPGIYTVKLTALYGFA
                     SDVAMKTNYISAAGGPELCLVTVTSEDANKLVWEKQTEIPVNYFNIYRETSQPGEYEV
                     IGTVDYEDSCVFADTTANPQEQPYKYRLSAFSLQGTESLPGNLHSTVHLSVNSVPGGC
                     ELEWTPYEGFVFDKYYIYRGNEADNLTLLDSAAGGVNSFTDADPPFGPLFYAIEIILD
                     GGCFPGGDEIYHTSRSNAIFAGNIGIPEDAGSTFRIFPNPVHDILNIQAGTGMIRQDA
                     EVSIYSVYGIELISCQLTTGRTAIDIRELAPGIYFINLRSRNQIKTGKFVVQ"
     gene            complement(4709..6406)
                     /locus_tag="PKZ74_06155"
     CDS             complement(4709..6406)
                     /locus_tag="PKZ74_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPM92376.1"
                     /translation="MHKNPGISDKWIRASVIGTTWAASEIVLGSFLHNLRIPFSSNIL
                     AGIGIIALVSVSYKWQQKGLFWRAGLICALMKTMSPSAVIFGPMIAIFSQALMLEIFT
                     RVLGRNFFGFAIGAMFATTWNLFQKIINFIIFYGFDIVDVYSGLLSYAQKQLNIRLDL
                     VWSPVILLAIIYSSLGLVSAIIGMRIGRKLLSQEPVAVKAGNNANLSFLKPKTQSFPY
                     SIGWLFLDMALIITAFILLNFGSWMVWGSGIIIITTIWVIRYKRALRQLARPTFWLYF
                     VLITMGTAFVINRVQSREFVEGLIIGIQMNFRAALVILGFSVLGTELYNPRIREFFLR
                     TSFKQLPMALELSFDSLPIMISAFPDIKEIVKSPVSVIYRVISQIDFRLEEIRRKLQR
                     KVFIISGPVGGGKTTQIRKIIEELWKKNIQVDGIYSPRVMEDGLTAGYDVVDVETGQR
                     EPFLRKAADSAHAAVGQYDIHPAGLELGLAALTRSHEKQCRLVVIDEVGRLEVNDRGW
                     SEKLALLIGQAQVLVLTVRDTYSDEVIKKWGLKDYEVFTATGDLYVKITDKIAGLTG"
     gene            complement(6427..8064)
                     /locus_tag="PKZ74_06160"
     CDS             complement(6427..8064)
                     /locus_tag="PKZ74_06160"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPM92377.1"
                     /translation="MSRNTTLPVRQSSAPEKSRMNLNLLYLVAFIEGGTVMAIELAGA
                     KMIAPFFGTSLFVWASVLAVTLGGLAAGYFAGGWATYRFPPLRLLFWELLAGTLLIAL
                     MPFVAVKFMSLTGSLGLRLGSLLSSFSFMFLPLFCMGMISPTLIQLQNRELKGTGKTA
                     GTVYAVSTLGGIIMTLLMGFYLLPEWGIRLSVFFTALLLASMAILVVIISRKFKGITG
                     ISVFVLVLLFLASAKSFSDANVAGKYLFRGEGILGQITVVETRGGDPVIRTLFINQIP
                     QTQVDAKYIPASYWKYPHRIAMIAGMKPPGSKALLIGLGGGNIAMELKQMGFSVDAIE
                     YDKRMVGLAERYFGFNPDGISVYVDDGRHFIRTTGERYDLVVIDVLNGEVQPHHMFTM
                     ESFGEMKRIMAPDGLLIINNQGFLWGEHGPGARSVYKTLAESGFKVKYYFAGDDKNSG
                     DIHFIASPADFRLPMEIPQNINACCREYPVDYPALVSAIEFGSAANIPDLRDAHILTD
                     DKPLLLTLYNYSNEEWRQNTMKSLLRGQSPPPVTFFH"
     gene            complement(8287..8586)
                     /locus_tag="PKZ74_06165"
     CDS             complement(8287..8586)
                     /locus_tag="PKZ74_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPM92378.1"
                     /translation="MEKLNNIHPGEILLEEFLIPLKISAYKLSKDIRIPQTRVSEILK
                     GRRRITADTALRLSNYFGNSAKFWLGLQDDFDIEEEILVKKDEFKVIRKFKENVA"
     gene            complement(8599..8880)
                     /locus_tag="PKZ74_06170"
     CDS             complement(8599..8880)
                     /locus_tag="PKZ74_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM92379.1"
                     /translation="MIQSFRSRETENIWNGIRSRKLPDEIQHIARRKLRMLNNSMNLN
                     DLIVPPSNNLEKLKGEFKDFHSICINDKWRIIFQWENGHSYNVEIIDYH"
     gene            9512..9961
                     /locus_tag="PKZ74_06175"
     CDS             9512..9961
                     /locus_tag="PKZ74_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92380.1"
                     /translation="MKKIITLVCMLLIGVSCVLAQYDCNVYWTYPPGADCTPKNLPAQ
                     GTYHIEVTLWIYDIANAEWVTPSGNPPINNESTSATSTNFSAAQCGVSDYCDESHDNT
                     PVFYLYGKVAFVDSSSQEFCSDNGQNTNGLSCNDFYNSLAAVQVTMN"
     gene            10452..>10866
                     /locus_tag="PKZ74_06180"
     CDS             10452..>10866
                     /locus_tag="PKZ74_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92381.1"
                     /translation="MGALIVQQPGSDHLYYVFTVGREWPYNIGMHYSVVDMHLDGGLG
                     DVTSEKNVLLAQAWAASNKLTSVRHANGEDIWIITRNFKSDQYYIAFLLTSSGLSTEG
                     VVSPVQWRDEFNNYGNMKISYDKKYLAAAYVSYKIE"
BASE COUNT         2880 a         2660 c         2629 g         2697 t
ORIGIN      
        1 ccgtggtcag ggcgcatgct gtcggatgaa gggatgatcc tccacttcgc caccccctcc
       61 tacaccgcag ggaccaaagg cgtgcagcgc ggccatgtgc tgatcgaacc gcgcacccag
      121 gccgagttcg accggatgaa aggacaactg aaaggcgcat gggtgctgat cctgggagag
      181 aacgacggct ggcccatcga tatctcggcc catgccgaca gcctgcgcga ttcgatcaag
      241 gtaaccaatg ccgcgatcga agaggagaac aataagatca ggcgggagaa cagggataac
      301 aaagagaaag ggctgcccga aaaagagctg aaagaactta acgaggaacc ggcgctattt
      361 taccgcgaaa tgcgcgaagc cggcatcctg ggcatcatcc agtcgtcgaa gacgccgatc
      421 cgcgtaatgt acgaccggaa gaacatcatg gacatgacct ttgataacct gccggcggtt
      481 cccgatataa agctggatga gcaccagtat gcggtcataa agcagatggc cgaagagcgc
      541 cgctatttcc agctcgaatt cgacatccgc aaccatttcc gccccgggcc gatcccctac
      601 tataacgtca tcggcgtcat tcccggcaca gagttccccg acgaatacgt catccagggc
      661 gggcatctcg acgcgtacga cgtagccacc ggcggcgtcg acgacggatc gggcgccacc
      721 ccgtcgatgg aagcagcccg gctgatcatg gcggcgggcg gcaaaccgaa acggaccatc
      781 ctgacgatcc tgtgggccgg cgaagagttc ggcctgctcg gctcccagag ctgggtcgac
      841 cgcaatatgg accagctcgg caagatctcc aacatattca accgcgacgg cgggccgacg
      901 gtgatcaccg ggatcagcgt gccggcagcc atgcgggaag actttgaaca aatctgcgcc
      961 ccgatcaacg gcatcaaccc cgattttccg ttcaaaatcg aagagcggca gccttccgaa
     1021 atgcccaaaa aagcctgggg cactgacagc ggcccgtttg ccgtcgctgg cgtccctgtg
     1081 ctccatttca acaccggcga tccgaaaggg tataatttta actactacga gatctggcac
     1141 accgaacgcg acacctacga caaaagcatc aaagaatacc aggaccacgt atcgatcgtg
     1201 acggctatcg tggtttacgg cgtggcaaac ctggatcatc tgctgaaccg tgaagggtac
     1261 tggaaagcgc cggaggagga acagggaaaa gggaaaaagg aaaaaggaaa agggggaaag
     1321 gaatagggaa aagaaaaaaa atatctgatt tttacttatt tatcttatct ttataggtct
     1381 gatatcaaat catgccatga aatccgttta tcgtccggtc attgcattgt tgatcttatc
     1441 catcgcatca tatgcagcta tcggccaggg catttccgtc aacaaaaccg gcgctccgcc
     1501 cgactcctcc gccatcctcg acatccaatc cacctcgcag ggcgtccttt ttccacggat
     1561 gtcgacctgg aaaataagaa atatccagga tccggaaacg ggcctcatgg tcttcaacac
     1621 cgattcgctc gaattgtaca tcttcaccgg ccagaaatgg gaatcggtcc gggataaaac
     1681 cgatacgatc acaacctggg agtgcggtca ggaatttctt tacgggggac aagcctacag
     1741 caccgtattg atagggactc aatgctggat gtcggaaaac ctcaatatcg gtgtcagggt
     1801 agaagggaca gtggaacagc atgacaacga taccattgaa aaatactgtt acaacaacga
     1861 cccggcgaat tgcgatgtct atggcgggct ctaccagtgg gccgaagtgg tgcagtatct
     1921 gaacggggcg accaatttca cctcgtggga tccggcgccc gaaggacctg tcaggggaat
     1981 ctgcccggaa gactggcata tcccctctta cggcgaatgg atggaattgg tggtatattg
     2041 cgattattgg ggcggacggc tgaaagagac gggaacggag cattggtttc cgcccaatac
     2101 ggatgcaaac aacgagagcg gttttaccgc tttcccgggc ggttaccggc acccatccaa
     2161 cggcggttat gccgaattcc ggtatatggg cacgttatgg tcaaccacag ggtatgaacc
     2221 ctatgaaacc tactcggtat attttatccg cttatatttt aataatggcg gaataacctg
     2281 ggcaatcggc atcgaaaaat cctgggggtt ctcggtgagg tgcgtgatgg ataattaagt
     2341 cgttggccgc tgtcctgtgt ccgccaaagg cggacgaagg aactctggtc gttagtcatt
     2401 agaagcatgc ataagatact cattattata ggattaatag ggatttgcgc caatatctat
     2461 tgccaggaag aagcgtataa ctggtatttc ggcgaacagg cgggtttgag ctttgcccag
     2521 ggtgatccgg tggcattgac cgacggtgca ctttatacag gtgaaggatg ttcggctatc
     2581 tcttcgtctt ccggggaact gcagctctac tccgacggca ggtttgtgta tgaccgcaac
     2641 cattccatca tgccgaacgg cagcggcctg ctgggacatt cttccagtac ccagtcgtgc
     2701 atcatcgttc caagaccggc caataattct caatactacg tatttaccgt tgatgccaaa
     2761 gacaacgggc tggttaacgg cctttgctat tcccttgtcg atatgacgct gaatggcgga
     2821 ctcggagatg tgtcgccggc agaaaaaaac atctccctcc ttccgtatac ctgtgaaaaa
     2881 gtaactgccg tcaggcacaa tgatggcgcc tctgtctggg tcattaccca ccaatggggg
     2941 tccaatgcat tttattcgtt cctcgtcacc gcagaaggtg taaatatgac gccggtcatc
     3001 agtcatgccg gtcaggccgt ttcaggcgac atggaggcgg ccaagggtta tatcaaagtt
     3061 tctcccgacg gcgccagaat tgctatggcg aacaatacgg cttttactgt cggtatcttt
     3121 agcttcgatg atgcatcggg agttgtgagt catatcatca ctgacgatag ttatgtgaat
     3181 ccgggaggat ccgaccccgg agggccttat ggcgtagaat tctcgccgga aagcgacctg
     3241 ctctatatta gcgaatggaa aacaaaccgg aagatcagcc agtacgacct ctcttccaat
     3301 gatccgcaga ctatcctgaa ttcacgcgtt accgtcgcca ctgtcaacca gaatgccgat
     3361 cctttcggag ccatccagct tgctcccgat aaacggcttt atatcgcacg aatgggaagc
     3421 aactatatgt cggccatcaa ttttcccgat acggcaggcc ccggctgcaa tttcaacgat
     3481 aatgccattg aactgacagg aggaaattca agatatgggc ttccggcctt tgttcaatct
     3541 tactttaacg atattgccgc tttcaccgct actcccctga aggattatgc ccctttgacg
     3601 gtccagttca cagatctttc cagtggcagc ccgaccggct ggaagtggga ttttgagaac
     3661 gacggcactt tcgatgcatt tattcagaac ccttcacata cgtatgatgt gccgggtatt
     3721 tacactgtaa aactgacagc gctttatggt ttcgcttccg atgtggctat gaagacaaat
     3781 tatatctcgg ctgcaggagg tcctgaatta tgcctggtga cagtcacatc cgaagatgcc
     3841 aataaactgg tttgggaaaa acaaacagaa attcccgtga attacttcaa tatttaccgg
     3901 gaaacgtcgc aacccggaga atatgaagtg atcgggaccg tcgattatga ggattcatgc
     3961 gtgtttgccg atactaccgc gaatccgcag gagcagcctt ataaatacag gttatcggcc
     4021 ttttcactcc agggaacgga atcattgccg ggaaatctcc atagcacggt tcatttatcc
     4081 gttaattcag tccccggtgg ctgtgaactg gaatggacgc cctatgaagg atttgtattt
     4141 gacaaatatt acatttatcg cgggaacgaa gcggacaacc tgactttgct cgattcggcc
     4201 gcaggtggag tcaattcctt taccgatgcc gatccgcctt tcgggcccct gttctatgca
     4261 attgagatta ttcttgatgg tggttgtttc cctggcgggg atgaaattta ccacacctcc
     4321 cgctccaatg cgatatttgc cggaaatatc ggtattccgg aagatgccgg atcaacattc
     4381 cggatctttc caaaccctgt tcatgatatc ctgaatatcc aggccggaac cggaatgatc
     4441 cggcaggatg cagaggtatc catttatagc gtatatggta tcgaactgat ctcctgtcag
     4501 ttaacgaccg gaagaactgc catcgacatc cgggagctgg cccccggtat ctatttcata
     4561 aatctgagat cccggaacca gataaaaaca gggaaattcg tggtgcagtg atgctgcctt
     4621 cttcacttta ggttgttggt caacttattt cagaagcaaa gcaatacggt tttgaaatga
     4681 aatactatct aattaatatc cctgaatatc aaccggtcaa accggctatc ttatcggtaa
     4741 tcttaacata aagatcgcca gtcgcggtaa aaacttcgta atcctttaat ccccacttct
     4801 ttattacctc atcggaataa gtatccctga cggttaaaac cagtacttgg gcctggccga
     4861 tcagcaaagc aagcttctcc gaccatcccc tgtcattcac ctccaggcgt ccgacttcgt
     4921 cgataacgac gagcctgcat tgcttttcgt gcgagcgggt cagcgccgcc aggcccagct
     4981 ccagtccggc cggatggata tcgtattgcc cgacggccgc gtgcgcacta tccgccgcct
     5041 ttctcaaaaa cggctcccgc tggccggtct ccacatccac cacatcataa cccgcagtca
     5101 ggccgtcttc cattaccctc ggagaataaa taccatcgac ctgaatgttc tttttccaga
     5161 gttcctcaat tattttccgg atctgtgttg tctttccccc cccaaccggt ccggagatta
     5221 taaaaacctt tcgctgcagt tttctcctga tctcctccag cctgaaatct atttgtgaga
     5281 tcacccggta aatgaccgat accgggcttt ttacaatttc ctttatatcc ggaaaggcag
     5341 aaatcatgat cggcaggctg tcgaaggaga gctccagggc catcggaagt tgtttgaaag
     5401 aggtcctaag gaaaaactcc ctgatccggg gattgtaaag ctcggtgccc agtacggaga
     5461 atcccaggat gacaagggcc gcccggaaat tcatctggat gccgatgatc agcccctcga
     5521 cgaactcccg tgactggacc cggttaatga caaacgccgt acccatggtt atcagaacga
     5581 aatagagcca gaaagtgggg cgggccagct gcctgagcgc ccttttataa cgaatgaccc
     5641 agatcgttgt aatgatgatt atccctgatc cccaaaccat ccatgacccg aagttcagca
     5701 ggatgaaggc ggtgatgatc agggccatat cgagaaacaa ccagccgatg gaataaggaa
     5761 aagattgtgt tttgggtttc aggaaggata aattggcgtt gttgccggcc ttaacggcca
     5821 caggttcctg cgacagcagt tttctcccga tccgcatccc gatgatagca gaaacaaggc
     5881 ccaggctgct gtaaatgatg gccaggagaa tgaccggcga ccagaccagg tcgagccgga
     5941 tattcagttg tttctgcgca tagctcagca agccggaata cacatccaca atatcaaaac
     6001 cgtaaaaaat aatgaagttt atgattttct ggaagagatt ccatgtagtg gcgaacatgg
     6061 cccctatggc aaatccgaag aaattcctgc ccagcacgcg ggtaaagatc tccagcatca
     6121 gggcctggct gaaaatagcg atcatcgggc cgaaaatgac tgcgctgggc gacatggtct
     6181 tcatcagggc acagatcagc ccggcccgcc agaacaaccc tttctgctgc catttgtaac
     6241 tcaccgatac cagggcaata atcccaatcc cggccaggat attcgaactg aaaggaatgc
     6301 ggagattgtg caggaaactt cccaggacga tctccgaggc ggcccatgtg gtgccgataa
     6361 cggaagccct gatccatttg tcgctgatcc ccggattttt atgcattcac gacaggtttt
     6421 tccgcattag tgaaaaaacg tcaccggcgg cggactctgg ccccgcagca ggcttttcat
     6481 ggtattctgc cgccattcct cgttggaata attgtacagg gtgagcagca gcggtttatc
     6541 atcggtcagg atatgcgcat cgcgcaaatc ggggatgttc gcagccgacc cgaattcaat
     6601 ggcgctcacc agggccgggt aatccaccgg gtattccctg cagcaggcat tgatgttttg
     6661 cgggatctcc atgggcaggc gaaaatcggc cggagaggca atgaagtgaa tatcgcctga
     6721 attcttatcg tcgccggcga aataatactt caccttgaag cccgattccg ccagcgtctt
     6781 gtaaaccgac cgggcgccgg gcccgtgctc tccccataaa aacccctggt tgttgatgat
     6841 cagcaggccg tcgggtgcca tgatccgctt catctcaccg aaagattcca tcgtgaacat
     6901 atggtggggc tggacctctc cgttcagcac atcgatgact accaggtcgt atctctctcc
     6961 ggtggtccgg ataaagtgcc ttccatcatc cacataaaca ctgatcccat ccggattgaa
     7021 gccaaaatat ctctccgcca gcccgaccat tcgcttatcg tattcgatgg catcgacgct
     7081 gaagcccatc tgtttgagtt ccatggctat attgcccccg cccaggccga tcagcaaggc
     7141 tttggagccg ggtggtttca tgccggcgat catggcgata cggtgcgggt atttccagta
     7201 agaagccggg atatatttcg catcgacctg cgtctggggg atttgattga tgaagagcgt
     7261 gcggatgacg ggatcgccgc cgcgtgtctc cacaacggtg atctgcccta agatcccttc
     7321 gccccggaaa aggtatttgc ctgcgacatt cgcgtcggag aatgactttg ccgaagccag
     7381 gaacaggagc acgagaacaa acacggagat accggtaatc cccttgaatt ttctgctgat
     7441 gatgaccacc aggatggcca tggatgccag caggagggcc gtaaaaaaga ccgacagccg
     7501 gattccccat tcgggcagga ggtagaagcc catgaggagg gtcatgatga tcccgccgag
     7561 ggttgagacg gcatagaccg tcccggctgt tttgcccgtt cctttcagct cccggttctg
     7621 caactggatg agggtgggcg agatcatgcc catgcagaac agtggcagga acatgaacga
     7681 aaacgacgaa aggagggaac cgaggcgcag gcccaggctg ccggtgagcg acatgaattt
     7741 gaccgccaca aaaggcatca gtgcgatcag cagtgtgccg gccagcaact cccagaaaag
     7801 cagtcgcagc gggggaaagc ggtaggtggc ccagcctccg gcgaaatagc ctgcggcaag
     7861 ccctccaagc gtcacagcca gcacggaagc ccatacaaag agcgatgtcc cgaagaaagg
     7921 cgcgatcatc ttggctccgg ccagctcgat cgccatcacg gtaccgcctt cgatgaacgc
     7981 gacaaggtag aggaggttca ggttcattct gctcttctcc ggggcgctgc tctgccggac
     8041 cggcagggtg gtatttcttg acattcagta aagaatttca gatttattct tcaaagatag
     8101 aaatcatatg cgattcttcg gcccggggcc ggggaatgct tatgaaccgt tcttccattt
     8161 gttttctcat cggaaattgc ttttggttat ggatgtagta aaggtagtta aatcttttcc
     8221 aatccagtca ttcgggtcaa tgaaaagaaa caaggtcaaa gttttagaaa ttgttgatgc
     8281 gtgaggttag gccacgtttt ctttaaactt tctgattact ttaaattcgt ctttcttaac
     8341 taaaatttct tcttcaatat caaagtcatc ttgcagtccc agccagaatt ttgcagaatt
     8401 accaaaataa ttactcaacc tgagtgcagt gtcagccgta atccttctcc tgcctttcaa
     8461 gatttcactt accctggttt gtgggattct aatgtctttc gataatttgt aagcagaaat
     8521 ttttaaagga attaaaaatt cctcgagcag aatctctccc ggatgtatat tatttaactt
     8581 ttccatattc ctattacatt aatgataatc tattatttcc acattataag aatgtccatt
     8641 ttcccattga aaaatgatcc tccatttgtc atttatacat atgctatgaa agtctttaaa
     8701 ttcacctttg agtttctcca gattattaga aggaggaacg attaaatcat tcaaattcat
     8761 cgaattattt aacattctaa gttttctcct ggcaatatgt tgaatttcat ctggtaactt
     8821 cctggatcgg ataccgttcc aaatattttc agtttccctg gatctgaaag attgaatcat
     8881 agtaacgtat tacaatacta acggcaaagg taagcaaaat attacaaaag accaaataga
     8941 aaatgtggcc tgacgctcag cgcaactgcc ggccggtgtt tttggccgca caggcgggcg
     9001 tacgatagtt caaagttaag aattctaagt tcaaagtacc acagcgcccg cctgggcgga
     9061 caagattttt tcaaaactgg ctggcagttg cgtttttgtt agccccagta atttcaaatc
     9121 gaaaatggca aattaataga agagctgttc gccctatctc accctctcag aaagtaagat
     9181 atatccggtt caaaaatatt tatcaattac acagtacatt ttgcgatatt tatgattatc
     9241 tttaggctcc cctagttaat gaccaggaaa ataaaatatt gtcaaatttc ttacctgctt
     9301 gcgaattccc atccgccatg aagggccagc aacactagga aaatttataa aattctgatc
     9361 aatcaaattc ctttattctc ttgtaacgac aggatttaga gaggaagttc caatctatac
     9421 tcgtatttat caattttcct atttttaatg caggtattaa tattaaacgc aaccaaataa
     9481 catatttata aaccttttag atcttaatca aatgaaaaaa attatcacct tagtttgcat
     9541 gctattgatc ggagtaagtt gcgttcttgc tcaatacgat tgtaatgttt actggactta
     9601 tcctccagga gcagattgca caccgaagaa tcttcctgcg caaggcacat atcatattga
     9661 agtaacactt tggatatatg atattgccaa tgctgaatgg gttaccccat ctggtaatcc
     9721 acctattaat aacgaaagca caagcgctac atcaacaaac tttagtgcgg ctcaatgcgg
     9781 ggttagcgat tattgtgatg agtctcatga taatacgcct gtcttctatc tttatggtaa
     9841 agttgccttt gtggactctt catcacaaga attttgttca gataacggac aaaatacaaa
     9901 tggcctctct tgtaatgatt tttataatag cttagctgca gtgcaggtta cgatgaattg
     9961 attcgatgtt attgttgcaa gagtttgttt taaaagccgc agcagtctcg attttatttt
    10021 gctccaatag gtaaacatgt tgattaaatg gttgttgcaa aaatcgaaac gatgaggtta
    10081 tccataattg taagtaattt gatagtgtct tccggctttt tagaccacct ttggattcat
    10141 tacgagaata catttgattg atgaggaaat tgaaatatta ccttttgtta ctggcttgta
    10201 tattgtttta caggacttat acccaaaacc aggctaacct ctggtactgg agtcaatact
    10261 gtggaataga tttcaattca ggtatccctg tatatcatga tcagacaatt attgcctcgg
    10321 gaaatgccat gtctgttatg tgtgatacca atggtaatct actcttttgt tgcagtggca
    10381 tacaagtaaa gaacagggaa gggcacacca tgccaaatgg tgagaatctt ataggatttg
    10441 aagctgcttg tatgggtgct ttaattgtac agcaacccgg atcggatcat ttgtattatg
    10501 tgtttactgt tggaagagaa tggccctata atattggcat gcattattct gtcgtggata
    10561 tgcacctgga tggagggctg ggcgacgtaa cctcagaaaa aaacgttctg ctggcacaag
    10621 cctgggcagc aagcaataaa cttacatcgg tccggcatgc caatggggag gatatctgga
    10681 tcattacacg taacttcaaa tcagaccagt attatattgc ttttttgttg acatcaagcg
    10741 gattatcgac cgagggagta gtaagccctg tccaatggag agatgaattc aacaattatg
    10801 gaaacatgaa aatctcatat gacaagaaat acttagccgc tgcatatgta agctataaaa
    10861 tagaga
//
LOCUS       DAOPNH010000089        10788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6560_length_10788_cov_7.006056, whole genome shotgun sequence.
ACCESSION   DAOPNH010000089 DAOPNH010000000
VERSION     DAOPNH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_10788_cov_7.006056"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PKZ74_06185"
     CDS             complement(<1..1399)
                     /locus_tag="PKZ74_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM92382.1"
                     /translation="MEYRFKSYLDLPVFKPLLAAVKQYGHPAYVVGGYVRDRILSRTT
                     KDIDVVVIGSGIELAEIYAQKIGKGASVNVFRNFGTAMVRFHEQDVEWVVEFVGARKE
                     SYRANSRKPIVEDGSLEDDQNRRDFTINTLAISLNSKDLGTLLDPFGGFHDMEECLIK
                     TPLSPDKTYSDDPLRMMRAIRFATQLDFVIEKGSFESIRRNADRINIISRERINDELE
                     KIILSKKPSVGFNLLMDSGLLKLIFPEFVALKGAEYIDGIGHKDNFHHTLQVLDNVAA
                     KSDNLWLRWGAILHDIAKPVTKKFEQGAGWTFHAHEFVGAKMIPKLFQRMRMPVDERM
                     KYVQKMVLLHLRPIVLSQEEVTDSAVRRLLFDAGDDIDDLMLLCEADITSKNREKVIR
                     YMKNFELVRKKLVEVEAKDRIRNWQPPVTGELIMETFGIPPGKEVGIIKNAIREAILD
                     GIIHNDFDEAYRLMIA"
     gene            complement(1439..2044)
                     /locus_tag="PKZ74_06190"
     CDS             complement(1439..2044)
                     /locus_tag="PKZ74_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92383.1"
                     /translation="MKIRTLPLLFSFLILSTWMFTSCSVLQQTSEMKNFGKCDFRIES
                     ARNMKLGGVSMQDKTSISQVGILEITKIGAAVASGTLPLTFDLNISAKNPNPTLAAMN
                     KLDWILIIDDAEMTRGVLNQRVEIPANSTTQFPVLINFDLMKSLNGKSADALINFVLN
                     LAGTGTKPTRIKLKAKPTILIGSTPLEYPGYITIRQEFGAQ"
     gene            2089..3072
                     /locus_tag="PKZ74_06195"
     CDS             2089..3072
                     /locus_tag="PKZ74_06195"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPM92384.1"
                     /translation="MLRFIQRQETDPYYNLAAEEHIFQSAAEDTFMTWRNNPAVIIGK
                     HQNALKEINTDFVEKYQIPVIRRITGGGTVYHDPGNINFSFIFTQRRENLIDFREFTR
                     PIILFLHSLGLDAAFEGKNNITVDGWKVSGNSAHLYKNKILYHGTLLFDTDHERLEKT
                     IRGNERLFKDKSVGSIRSKTINIKEITKNEISMDQFISQFQKFIFNYFEGYSLHILNE
                     EDRSSIKKLVDTKYKSYEWNFGYSPDYVFNNRFYFKDEEYAVSIQVKKGKIELADIKG
                     PATNIITLNQLETLLSGLYHQKSSIRKAFDQIPVKTPSLTEFLNFLISKLF"
     gene            3099..3905
                     /locus_tag="PKZ74_06200"
     CDS             3099..3905
                     /locus_tag="PKZ74_06200"
                     /inference="COORDINATES: protein motif:HMM:NF018731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HPM92385.1"
                     /translation="MNLEQTFNKLWEGYTSQNPAVQQVYDLFIREGERVVNDHIAFRT
                     FNDPRVNIDVISKPFLSNGYVEKGTYRFEEKKLFAKHYEHKKDPEAPRVFISELLIQN
                     FSLFLQESVLQALDQISLERIDADGLIFSGRIWEKPSFEIYSKLKEESEYAAWVYVFG
                     FCANHFTVSVNYLQRHNTLEKVNSLLKSSGFLINDAGGEIKGTPEELLEQSSIKSGIV
                     KVRFVEGDFEIPGCYYEFARRYPDENGKLYSGFIAKSADKIFESTNFYKK"
     gene            3935..6889
                     /locus_tag="PKZ74_06205"
     CDS             3935..6889
                     /locus_tag="PKZ74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPM92386.1"
                     /translation="MSPDSLNTSLEALRRSLEGDLFTDESYRILHATDASAYREMPIA
                     VARPRTKEDIRKLVTFACENGTSLIPRTAGTSLAGQVVGNGIVVDVSKYLNNILEINA
                     EERWVRVEPGVVLDELNMAVARFSLFFGPETSTSNRCMIGGMVGNNACGAHSIIYGST
                     RDHLISLKAILSDGSDAEFKPITPEEFERRCDGDSLEAAIYRNIRELLKEPGVQEEIR
                     REFPDPKIKRRNTGYAIDQLLEYYPFSANGQPFNFCKILAGSEGTLAFSTEIKLNLVP
                     LPPKQKILLCAHFKTLEEALQGNLVALKHNPGAVELMDKFILDCTRDNIEQRKNRFFI
                     EGDPGAILIIEFARETREELEAITSKITEDFRSAGLGYHYPAVTGADIPRVWALRKAG
                     LGVLTNIPGDAKPVSVIEDTAVNPENLPQYIAEFKEILAGHGLECVYHAHIATGELHL
                     RPVLNLKDPADVKKFRAIATDTARLVKKFRGSFSGEHGDGRVRGEFIPLMIGEKNYEI
                     LRKIKQAWDPGNIFNPGKITGTAPMDTSLRYEPGRATRQFKTVFDFSHDGGILRAAEK
                     CNGSGDCLKSHLIGGTMCPSYMASKDENATTRARANILREFLTRSDRKNPFSHKEIYD
                     VMDLCLSCKGCKSECPSNVDIARYKAEFLQHYYDEHGVPLRSLAVANITRLNRLGAIL
                     PGVYNFMVRNQTISGLMKKVLGMAPQRPLPALHPYTLNTWLNKYNQILPSGLPQRQVW
                     LFIDEFTEFNDVPVGIATVKLLNALGYKVLTCQHPESGRTYLSKGLVRAARKYAIDNV
                     MTFKDKVSEDVPLIGIEPSAILSFRDEYPDLVGPELKDFATKLSKNCLMFDEFFMREI
                     KSGNISKDHFTRSKANIKLHGHCHQKALSTTGPTKEMLSFPENYTVEEIPSGCCGMAG
                     SFGYEKEHYELSMKVGELVLFPAVRQSDQTFIIAAPGTSCRHQIKDGTGREALHPVEV
                     MWSAIDPSLRPG"
     gene            complement(6940..7851)
                     /locus_tag="PKZ74_06210"
     CDS             complement(6940..7851)
                     /locus_tag="PKZ74_06210"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPM92387.1"
                     /translation="MSFTVTILGSGAAIPTGDRNPTAQVVKVRNRTLLLDCGEGTQVQ
                     LRKAGLRIQKINYIFISHLHGDHYFGLIGLINTMHILGRKDALHIFAIEPLKRLIDLQ
                     LELSNTTLVYPLVFHPIDPEINRTIYEDEQISVSTIPMNHRVPTCGFLIQEKSGLRRI
                     RKDFARSVKVPVEYYERIRRGEDFTDENGKHYPNHSITDESTAPKSYAYCTDTAYHEP
                     VIPIIRDCDLLYHEATFMEDKSADAKAKFHTTAREAATIALKSNAKKLVIGHFSARYK
                     DSDGLLREAQEVFPETMIAEEGKTFQV"
     gene            complement(7929..10205)
                     /gene="rpsA"
                     /locus_tag="PKZ74_06215"
     CDS             complement(7929..10205)
                     /gene="rpsA"
                     /locus_tag="PKZ74_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPM92388.1"
                     /translation="MFLMIEKDQEVNNQDEIQEPSEVNETPEAAEAEMMAEIPAEEPA
                     AVETPEETAEIAEEPEAIPEEPAETPEEPASEPEAPEEFIPVEDEPVKEIFGEVPVVR
                     EAPKPNPDFDWNAIGKKQEKYSDDERNRLEKIYDKTLSSIAEHEVIEGTVVGKNNREI
                     VVNIGYKSDGVIPVSEVRYLTNIKNGDKLDVYVESQEDLGGQLVLSHKKARILKSWER
                     VNSAFENDEIITGYVKCRTKGGLIVDVFGIEAFLPGSQIDVKPIRDYDIFVDKTMEFK
                     VVKINQEYKNVVVSHKALIEAELELQKTEIMSKLEKGQVLEGTVKNITSYGVFIDLGG
                     VDGLIHITDLSWGRINHPEEIVELDQKIKVVILDFDDEKKRIALGLKQLMPHPWESLD
                     ENLKIGDKVKGKVVVIADYGAFIEIAAGVEGLIHVSEMSWSQHLRTAHDFLKVGDEVE
                     AIILTLDREERKMSLGMKQLVPDPWENIVQKYPINSKHTATVRNFTNFGIFVELEEGV
                     DGLIHISDLSWSKKIKHPAEFTKIGEQIEVIVLDVDTENRRLSLGHKQLEENPWDVFE
                     SVFTLGSVHQGTVVSANDKGFIVSLPYGVEGFVPTRHAAKADNSMAKVDDNLDFKVIE
                     FSKENKKIILSHARVHQDEARAERDKEDGKVKREQRTTNKAVKKVQDNVEKTTLGDLE
                     ALVSLKTDMEKQEKLENKEKLEKLEKKAKAEKAAKAEKEATAEKPEKEEKKSKGKAKA
                     EPKAEEAPEIKFDAEPEA"
     gene            10495..>10788
                     /locus_tag="PKZ74_06220"
     CDS             10495..>10788
                     /locus_tag="PKZ74_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPM92389.1"
                     /translation="MPLKCGIVGLTLVGKTTIFNCFSNARAQSGGALKANIGTIHVPD
                     QRLHEIDKFIKSVKIVPATVEIVDIPGLTKGGGQESGGNKFLADIQQTDAIIHV"
BASE COUNT         2637 a         2695 c         2596 g         2860 t
ORIGIN      
        1 ctgcaatcat cagccggtac gcttcatcga agtcgttatg gattattccg tcgaggatcg
       61 cttcgcggat ggcattttta atgatcccca cctcttttcc cggcgggatg ccgaatgtct
      121 ccatgatcag ctcgcccgtg accggtggct gccagttcct gatccggtct ttcgcctcta
      181 cctccaccag ttttttcctc accagctcga agtttttcat ataccggatc accttttcac
      241 ggttcttgga ggtaatatcg gcttcgcaaa gcagcatcag gtcgtcgata tcgtctccgg
      301 cgtcgaacag caggcgcctg acggccgaat cagtcacctc ttcctgtgaa agaacgatcg
      361 ggcggaggtg cagcagcacc atcttctgga catatttcat ccgttcatcc accggcatgc
      421 gcattctctg aaacagcttc gggatcatct tcgcgccgac aaactcgtgg gcgtgaaatg
      481 tccagccggc gccctgttca aacttctttg tcaccggttt ggcaatatcg tgtaaaatgg
      541 ccccccagcg cagccagaga ttgtcagatt tagccgcaac attgtcaagt acttgcaacg
      601 tatggtgaaa attatcctta tgcccaatcc cgtcgatgta ttcagcccct ttcaacgcca
      661 caaactccgg aaagatcagc tttaacaggc cggagtccat cagcaggtta aacccgacgg
      721 agggcttttt cgagagaatt atcttctcca gttcatcgtt gatcctttcc cgggaaatga
      781 tgtttattct atccgcattc ctgcggatcg attcaaatga gcctttttca atgacaaaat
      841 caagctgggt ggcaaaacgg atggcccgca tcatcctcag cgggtcgtcg gaataggttt
      901 tatcgggtga aaggggtgtt ttgataaggc attcttccat atcgtgaaat ccgccgaaag
      961 gatcgaggag cgttcccagg tctttgctgt tcaggctgat agccagcgta ttgatcgtaa
     1021 aatcgcgccg gttctggtcg tcttccaggc tcccgtcttc cacgattggt ttgcgtgaat
     1081 tggcccggta ggattctttc ctggccccga caaactcaac gacccactct acatcctgct
     1141 cgtgaaacct gaccatggcg gtgccgaaat tgcggaaaac gtttactgat gctccctttc
     1201 cgattttctg cgcatagatc tccgccagct cgatgccact gccgatcacc acaacatcga
     1261 tatctttggt cgtgcggctc aggatccggt cgcggacata cccccccaca acataagcgg
     1321 ggtgaccgta ttgcttcacg gccgccagca acggcttgaa tacgggaaga tcaagatatg
     1381 atttgaagcg gtattccatc agcggatgca aaagtaagca aaacgggcaa atcccggctt
     1441 actgtgcccc aaattcctgc cggatggtaa tgtatccggg gtattccagc ggggtggaac
     1501 cgatgagaat ggtcggtttt gctttcagct tgatccgcgt gggtttggtg cccgtcccgg
     1561 ccaggttcag cacaaaattg atcagggcat cggccgattt tccattgagt gatttcatca
     1621 ggtcgaaatt gatcagaacc ggaaactgtg tcgtggaatt agccggaatt tccacccgct
     1681 ggtttaaaac tccccgggtc atctccgcat cgtcgatgat cagtatccag tcgagcttgt
     1741 tcatcgcagc caatgtcgga tttggattct tagccgagat gttcaggtcg aaggtaagtg
     1801 gcagcgtgcc ggaagccacc gcagcgccta tttttgttat ttcgagaata ccaacctgcg
     1861 aaatggaggt tttatcctgc atgctcaccc cgcccagctt catattgcgg gccgattcga
     1921 tccggaaatc acacttgccg aaatttttca tctcggaggt ttgctgtaga acggaacatg
     1981 aggtgaacat ccacgtcgag aggataagaa aagagaaaag taaaggcaat gttctgatct
     2041 tcatgatttt ttatttaaat agtatagctt tgcaacaatt tcaatctgat gctgcgcttt
     2101 attcaacgcc aggaaaccga tccttattat aaccttgctg cagaagagca catttttcag
     2161 tccgcggcag aggatacctt tatgacctgg aggaataatc ctgccgtcat catcggaaaa
     2221 catcagaatg cgctgaagga gatcaatacg gatttcgttg aaaaatatca gatcccggtc
     2281 atccgaagga tcaccggggg aggaactgtt taccatgatc cgggcaacat caatttctcc
     2341 tttattttta cccaaagaag agaaaatctc attgatttca gggaattcac ccggcccatc
     2401 attctttttc ttcatagcct gggactggat gcggcttttg aaggcaaaaa caatatcacg
     2461 gtggatggat ggaaagtttc cggcaattcc gctcacctgt acaaaaataa aatcctttat
     2521 cacggcacct tgttgtttga tactgaccat gagaggctgg aaaaaactat ccggggcaac
     2581 gaaaggttgt ttaaggacaa atcagtgggt tccataaggt ctaagactat aaatatcaaa
     2641 gaaataacca agaatgagat ctcaatggat caattcatat ctcaatttca gaaatttatt
     2701 tttaattatt tcgaggggta ttcgctgcat attctaaatg aagaggatcg gtcatccatt
     2761 aaaaaacttg ttgatactaa atataaaagt tatgaatgga acttcggata ctctcctgat
     2821 tacgtgttta ataatagatt ttatttcaaa gatgaagaat atgcagtcag tatccaggta
     2881 aagaaaggaa agattgaact ggctgatatt aaaggacctg caacaaatat aatcacatta
     2941 aaccagctgg aaactttact ttcaggcctt tatcaccaga aatcctctat acgaaaggcc
     3001 tttgatcaga ttcctgtcaa aacaccctcc ttgactgaat ttttgaactt tttgatctca
     3061 aaattgtttt gatcatctgt gaacttttgt aaaacattat gaacctcgaa cagacattca
     3121 ataaactttg ggagggatac acctcacaaa atcctgccgt gcaacaggtt tacgaccttt
     3181 ttatccggga aggcgaacgg gtggtgaacg accatattgc gttccgcacc ttcaatgatc
     3241 cccgcgtcaa tattgacgtt atctcaaaac ctttcctgtc caacggttat gttgaaaaag
     3301 ggacgtaccg ttttgaagaa aaaaaattgt ttgcaaagca ttacgagcat aaaaaagacc
     3361 ctgaggcccc tcgcgtattt atcagcgaac tgcttatcca gaacttcagt ctttttctgc
     3421 aagaatctgt tttacaagcg cttgatcaaa tttccttaga aagaattgat gcggatggtt
     3481 tgatcttctc cggaagaatt tgggaaaaac cctcattcga aatttacagc aaactcaagg
     3541 aagaatcgga atacgctgca tgggtctatg ttttcggctt ttgtgccaat cattttacgg
     3601 tttctgtgaa ttatctccaa cggcataaca cccttgaaaa ggtgaacagc ctgctgaaat
     3661 ccagcggatt cctgatcaac gatgcaggtg gtgagatcaa aggcacaccg gaagagctat
     3721 tggagcaatc ttccatcaag tcagggatcg tgaaagtcag attcgtcgaa ggcgatttcg
     3781 aaattcccgg ttgctattat gaatttgccc gccgatatcc ggatgagaac gggaaacttt
     3841 attcgggatt tatcgccaaa tcggccgata aaatctttga aagcaccaat ttctataaga
     3901 agtaaagcag aaaaaagggt taattttggc tcccatgagc ccggattcat taaatacctc
     3961 actcgaagcg ctgcgcagat cgctggaagg cgaccttttc accgacgaat cctaccgtat
     4021 tttgcatgcc accgatgcct cggcataccg tgaaatgccc attgcagttg cccgtccaag
     4081 aacaaaggag gatatccgca aactggtaac ttttgcctgc gagaacggca catcgcttat
     4141 tccgcgtacg gccggcacct ccctggctgg ccaggttgtg ggtaacggaa ttgtggtgga
     4201 tgtatcgaaa tatctcaata acatcctgga gatcaatgcg gaagagcgct gggtgagagt
     4261 tgagccgggt gtcgtgctgg atgaattgaa catggctgtt gcccgtttta gcctcttctt
     4321 cggtcccgaa acttccactt caaaccggtg catgatcggg ggaatggtcg gcaacaacgc
     4381 ctgcggggcg cattccatta tctatggaag cacacgtgac catctgatct cgcttaaggc
     4441 cattctgagc gacgggtccg acgcggaatt caaacctatt acgccggagg aatttgaacg
     4501 gagatgtgat ggggattccc ttgaagctgc aatctaccgg aatatccgtg aattgcttaa
     4561 ggagcccggc gttcaggaag agatccgcag ggaatttccc gatccgaaga taaaaaggcg
     4621 aaataccggg tatgccattg accagttgct tgaatattac ccatttagtg ctaacggtca
     4681 gccatttaac ttctgcaaaa tactggccgg ttcggaaggc acgctggcat tttcgacaga
     4741 aatcaaactg aatctcgttc cgctcccccc gaaacaaaag attctgctct gtgctcattt
     4801 caaaacgctc gaggaggcgc tccagggaaa cctggttgcg ctgaaacaca atcccggcgc
     4861 agtggaactg atggacaagt tcattctcga ctgcacccgc gataatattg agcagcgcaa
     4921 gaaccggttt tttattgaag gtgatccggg cgccatcctg atcattgagt ttgcgcgaga
     4981 gacccgggag gaattggagg ctatcaccag caaaattaca gaggatttcc ggtctgccgg
     5041 cctgggctat cactacccgg ccgttacggg tgccgatatt cccagggtat gggcccttcg
     5101 caaagccggg cttggagttc tgacaaatat acccggcgat gccaaaccgg tatcggtcat
     5161 agaagacaca gcggtgaacc ccgaaaatct accgcagtac attgccgaat tcaaagagat
     5221 tctggccggg cacgggctgg aatgcgtgta ccatgcgcat atcgccacgg gcgaactgca
     5281 cctgaggcct gtgctgaacc tgaaagatcc ggcagatgtg aagaaattcc gggctattgc
     5341 caccgacaca gcacggctgg ttaagaaatt ccggggttca ttcagcggcg agcacggcga
     5401 cggaagggtc aggggcgaat tcatcccgct gatgatcggg gaaaagaatt atgagatcct
     5461 ccggaagatc aagcaggcct gggatcccgg caatatcttc aatcccggca agatcaccgg
     5521 gactgcgccg atggacacca gcctgcgcta tgagccgggc cgggccacgc ggcaatttaa
     5581 gacggttttc gatttttcgc acgacggcgg tatcctgcgg gctgccgaga aatgcaacgg
     5641 ctcgggcgac tgcctcaaat cgcacctgat cggcggaact atgtgcccga gttatatggc
     5701 aagcaaagat gaaaatgcca ccacccgggc cagggccaat atcctgcgcg aattcctgac
     5761 acgctccgat cggaagaatc ccttcagcca taaagagatt tacgatgtta tggatctttg
     5821 cctttcgtgc aagggatgta agtcagaatg cccgtcgaat gtagatattg ccaggtataa
     5881 agccgaattc ctgcagcatt attacgatga gcacggcgtg ccgctgcggt cgctggcagt
     5941 agctaacatt acccggctga accggctggg agctatcttg cccggcgttt acaatttcat
     6001 ggtccggaac caaacgatct caggcctgat gaagaaagtc ctcgggatgg ctccccaacg
     6061 acccctaccc gctttacacc cttacaccct taacacctgg ctaaacaagt ataaccagat
     6121 cttgccttcc ggtctgcctc agcggcaggt ctggctcttc atcgacgagt tcaccgaatt
     6181 caacgatgtg ccggtaggca tcgccacagt caaattgctc aatgcactgg gctacaaggt
     6241 tctgacctgc cagcatccgg aaagcgggcg gacctatctt tcaaaaggtt tagttcgggc
     6301 tgccaggaaa tatgccattg ataatgtgat gacttttaag gataaagtga gtgaggatgt
     6361 tccgctgatc gggattgagc cttctgccat tttatctttc cgcgacgaat atcccgacct
     6421 ggtcgggccg gaattgaaag atttcgccac caaattatcg aagaactgcc tgatgttcga
     6481 cgagttcttc atgagagaaa taaaatcggg gaacattagc aaggaccatt tcacaagatc
     6541 aaaagccaat atcaaattgc acgggcactg ccatcagaaa gccctgtcca ccaccggtcc
     6601 gacaaaggag atgctttcat tccctgagaa ttacactgtg gaagagatac catcgggatg
     6661 ttgcggaatg gcgggctcgt tcggttacga aaaggagcat tatgaattat cgatgaaggt
     6721 cggtgaattg gtattgttcc cggcagtaag gcaatcagat caaactttta ttattgctgc
     6781 gcccggcacc agttgccgcc accagatcaa agacggaacg ggaagggaag ctttgcatcc
     6841 ggtggaagtg atgtggtcgg caatcgatcc ctcgctacgc ccgggatgac ggtttggaga
     6901 tttgaagtgc agctctatat ggaggcatat acaatatgct cacacctgga atgtcttgcc
     6961 ttcttcagcg atcattgtct ccggaaaaac ttcctgtgct tctcgcagca gcccatccga
     7021 atctttataa cgggctgaga aatgcccgat aaccagcttt ttagcattgg atttcaatgc
     7081 gatcgttgcg gcttcgcgcg ctgtcgtatg aaatttggcc ttcgcatcgg ccgacttgtc
     7141 ttccataaat gttgcctcat gatataacag gtcacaatca cggatgatgg gaatgaccgg
     7201 ctcgtggtag gcagtatcgg tgcaataagc gtaagatttc ggagctgttg attcgtctgt
     7261 aatcgaatga ttagggtagt gttttccgtt ttcatccgta aaatcctctc cccttctgat
     7321 ccgttcatag tattcgacag gaaccttgac actcctggcg aaatcttttc tgatccttcg
     7381 cagaccactc ttttcctgga tcagaaatcc gcaggtcggc acccggtggt tcatcgggat
     7441 cgtggaaacg ctgatctgct catcttcata aatagttcga ttgatctccg ggtcaatcgg
     7501 atgaaagaca aggggataca ccagggttgt attggaaagc tcaagctgaa ggtcgatcag
     7561 gcgtttcaga ggttcaatgg cgaaaatatg caaagcgtct ttccttccca ggatatgcat
     7621 ggtgttgatc aggccgatca atccaaaata atgatcgcca tgaagatgag aaataaagat
     7681 ataattgatc ttctgtatcc gcaaaccggc ttttctcaac tgcacctgtg tcccctctcc
     7741 gcaatccagc agcaaggtcc ggttgcggac tttcacaacc tgtgcggtcg gattcctgtc
     7801 acccgtaggg attgcagctc ctgatcccag tattgtcacg gtaaaactca tgattccaaa
     7861 aatagttaat ttataaaaaa agagcgcccc ggaaatcgaa gcgctctgtt attgggataa
     7921 tttcgggatt aagcttcggg ttcggcgtca aatttgatct ccggcgcctc ttcagccttg
     7981 ggttcggctt tagccttacc tttggatttt ttctcctctt tttccggttt ttcggctgta
     8041 gcctctttct cagccttagc tgctttttcg gctttagctt tcttctcgag tttttccagc
     8101 ttctccttat tctccagctt ctcctgtttt tccatgtcgg tcttcaggga aacaagggct
     8161 tcaaggtcgc cgagggtggt cttttcgaca ttgtcctgga ctttctttac agccttgtta
     8221 gttgtacgct gctccctctt cactttgccg tcttccttgt cgcgttctgc cctggcttca
     8281 tcctggtgga cgcgcgcatg gctgaggatg atcttcttgt tttccttgga gaattcaatg
     8341 accttgaaat caaggttatc atcaactttt gccattgagt tgtcggcttt ggcagcatgg
     8401 cgggtcggta caaatccttc aacaccgtaa ggcagggaaa cgatgaatcc tttatcgttc
     8461 gctgaaacga ctgttccctg gtgtactgaa ccgagcgtaa acaccgattc gaacacatcc
     8521 cacggatttt cctccagttg tttgtggcct aagctcaggc gtctgttttc ggtatcgaca
     8581 tcgagcacga tcacttcgat ctgttcgccg atcttggtaa attcggccgg gtgcttgatc
     8641 ttcttcgacc agctcaggtc ggagatgtgg atcaggccgt cgacgccctc ttccagttcg
     8701 acaaagatgc cgaaattggt aaaattacgg acggtagccg tatgcttgga gttgatcggg
     8761 tatttctgca cgatgttttc ccacggatcg ggcacaagct gcttcattcc cagcgacatc
     8821 ttccgctctt cgcggtcgag ggtgaggatg atggcttcca cttcgtcgcc gactttgagg
     8881 aaatcgtgag cggtgcgcag gtgctgcgac cacgacattt cggaaacatg gataagtcct
     8941 tccacgccgg cagcgatctc aatgaacgca ccgtaatcgg cgatgacgac cactttgcct
     9001 ttcactttat cgccgatctt caggttctca tccagcgatt cccacggatg cggcatcaac
     9061 tgcttcaggc caagggcgat acgcttcttt tcatcgtcga agtcgaggat aacgaccttg
     9121 atcttctggt cgagttccac gatctcttcc gggtggttga tacgtcccca cgacaggtcg
     9181 gtgatgtgga taagtccgtc cacaccgccc aggtcgatga acacaccgta cgaagtgatg
     9241 tttttgacgg ttccttcgag cacctgtcct ttttcgagct tcgacatgat ctcggtcttc
     9301 tgcagctcga gttcggcttc gatcagtgct ttgtgcgaaa cgacgacatt tttgtattcc
     9361 tggttgatct tcaccacttt gaattccatg gtcttgtcaa cgaagatgtc gtaatcgcgg
     9421 atcggtttta cgtcgatctg tgatccgggg aggaaagcct cgatcccgaa cacatccacg
     9481 ataagaccgc cctttgtacg gcatttcaca tagccggtga tgatctcgtc gttttcaaat
     9541 gcgctgttga cccgctccca tgatttcagg atgcgggctt ttttatgcga cagaacgagc
     9601 tgtccgccaa ggtcttcctg gctttcgaca tacacgtcga gcttgtcgcc gttcttgata
     9661 ttggtcaggt acctgacctc cgacaccggg attacgccat cggatttgta gccgatattg
     9721 accacgattt cgcggttgtt tttccctacg accgtgcctt ctatcacttc atgctcggca
     9781 atggagctta gggttttgtc atagattttc tccagccggt tgcgttcgtc atccgaatac
     9841 ttttcctgtt tcttcccgat agcgttccag tcgaagtcgg gattgggctt cggggcctcc
     9901 ctgacgacag gtacctctcc aaatatctcc tttaccggct catcctcaac gggaatgaat
     9961 tcctccggcg cctcaggttc tgatgcgggt tcttcggggg tttctgcagg ttcttcagga
    10021 attgcttccg gttcttctgc gatttctgct gtttcttcgg gggtttcaac tgctgccggt
    10081 tcttcggcgg gaatttcggc catcatttcg gcctcggcag cctcgggggt ttcatttact
    10141 tcggaaggtt cctggatttc atcctggttg tttacttcct ggtctttttc aatcattaag
    10201 aacataatgc gtttcgtttc atgcccctgc gccagatgac atttcacaaa acaggtttaa
    10261 cttcagtttt caaggttgca gcgcagattt caatacaacc ttccaaaatg gggtgcaaag
    10321 atagaatttt tagttttaag ttttgaggtt ttttagataa aaagttgaat cttcccacac
    10381 cttccaaata aacccgttac ctgtaacccg taactttgga actttggaac actggaacat
    10441 tgaaactttt tccagtaatt ccctatcttt gcaactcatc aacccagaac atccatgcca
    10501 ctcaaatgcg gaatcgtcgg actgacactc gtgggcaaga ccaccatctt caattgtttt
    10561 tccaatgcac gcgcccaatc gggcggagcg ctgaaagcca acatagggac gatccatgtc
    10621 ccggatcaac ggctgcatga aatcgataag ttcatcaaaa gcgtaaagat tgtcccggca
    10681 acagtcgaaa tcgtcgatat ccccgggctg accaaaggcg gcggacagga aagcggcggc
    10741 aacaaattcc tggccgatat ccagcaaacc gatgccatca tccatgtc
//
LOCUS       DAOPNH010000090        10716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6628_length_10716_cov_6.120439, whole genome shotgun sequence.
ACCESSION   DAOPNH010000090 DAOPNH010000000
VERSION     DAOPNH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_10716_cov_6.120439"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..685)
                     /locus_tag="PKZ74_06225"
     CDS             complement(86..685)
                     /locus_tag="PKZ74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92390.1"
                     /translation="MKYTIIKVVLSVIIVVLAYLIYESILRPVRFNKATSAREAKVIA
                     TLTDLRTSQQFYKRQHNKYTASFDTLISFLRNGEIPVVKMIPDPNDTTFTKTINDTLG
                     YVKVADSLFRARPGYNLDSLRYIPFSGGELFEIAASEIDRSGIKVGVYEIKAHYSTFL
                     KGLDRQLVINLIKSKEDIEKYPGLKIGSMEEPSTDGNWE"
     gene            complement(811..1299)
                     /locus_tag="PKZ74_06230"
     CDS             complement(811..1299)
                     /locus_tag="PKZ74_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPM92391.1"
                     /translation="MRWNSVLKNSPKIMIKLNFPEFEFKREIRDGKELIFDPVRRRFV
                     ALTPEEWVRQHAIRYLTGYKNVPVSLIRIEAVIKLFRTKKRFDLVVSDRNGNALLLAE
                     FKSPAVAVTQSVLDQAVRYNLALKVKYLVLSNGIQHVYCQVGNDPGSLKLLDDLPEFQ
                     FL"
     gene            1337..2107
                     /locus_tag="PKZ74_06235"
     CDS             1337..2107
                     /locus_tag="PKZ74_06235"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPM92392.1"
                     /translation="MKTKEEIVKNWLPRYTGRSLEDFGKYILLTNFDHYVELFANLHN
                     VEITGLDRPMPNATAGGITIINFGMGSANAATIMDLLSAIEPKAMLFIGKCGGLKKKN
                     EIGDLILPIAAIRGEGTSNDYFPPEVPALPAFNLQKAVSTTIREYNHDYWTGTVYTTN
                     RRVWEYDEEFKERLRRMRCMAIDMETATLFIAGFANSIPSGALLLVSDQPMIPEGIKT
                     EETDRQVIRDHVETHLRIGIDSLQLLINDGLTVKHLRF"
     gene            2113..3111
                     /gene="holA"
                     /locus_tag="PKZ74_06240"
     CDS             2113..3111
                     /gene="holA"
                     /locus_tag="PKZ74_06240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPM92393.1"
                     /translation="MTYDQITGDLRRKIYRPVYLLHGEEPYFIDAITAMIEESVLTES
                     EREFNQTIVYGRDTDPGKIIDIARRFPMMANYQVVIVKEAQDIDKIENLKPYVEKPVP
                     TTILVLAHKHKKLDQRKGLHKIIEKTGVLFESKKLYDDKIPAWINGQVQAKGYTIKPD
                     ACQLLAEYLGADLGRIINELEKLVISLPGGTAIDAALIERNIGISKDYNFIELQNAIG
                     NRDIHKANRIVNHFTANLKQNPTIALVAILYGFFIKLMIYHQLKDKSPKNAASDMGIH
                     PFFYKDYEKAAKNYSFRRIRPIIAVLREYDLRLKGVNNGSTEEAGLLRELVYKIMH"
     gene            complement(3115..4878)
                     /locus_tag="PKZ74_06245"
     CDS             complement(3115..4878)
                     /locus_tag="PKZ74_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPM92394.1"
                     /translation="MEVTRLFDLLPFYKTSFKPKDDVLAGKDGGKWIKYSIDQYIENV
                     NNISHGLLKLGVRPGDKIATISNNRPEWNFVDMAILQIGAVHVPIYPTISESDYKYIL
                     KHSEVSFVFISGRDILRKIEHILPEINAVQDVYTFKAIEGIKHLDELIALGQTNRQSE
                     KLEKLKEAVNPDDLATLIYTSGTTGFPKGVMLSHRNLLSNVMGVKHIFPVDENCKGLS
                     YLPLCHVYERMDIYTYHYLGLSIYYAENMGTIADNIREIRPEIFTTVPRLLEKVYDKI
                     IAKGSQLKGFKKAIFFWAVSLANRYELEGKGWLYHAQLRLANKLVFVKWREALGGNVR
                     VIVSGGAALQPRLARVYTAAGIPVLEGYGLTETSPVLAVNTLEEGGRMFGTVGKPLKN
                     TEVKIGPDGEILARGANIMQGYFKEPGMTAEAIDADGWFHTGDVGILEPEGHLRITGR
                     KKEIFKTSLGKYISPQLLENKFKESAFIDGILILGENQKYAAALVVPDFNHLKSWCEH
                     KNMPYTTDKEMIKNPLVVKRFKKEIDHFNKFFGSTEQIQSFELIDHEWSLETGELTAN
                     LKLKRSYILDKYKEEVSRLFR"
     gene            complement(5008..5766)
                     /locus_tag="PKZ74_06250"
     CDS             complement(5008..5766)
                     /locus_tag="PKZ74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM92395.1"
                     /translation="MIKTILITGATAGIGKATAFRFAQEGHRLIITGRRIKILQDIAT
                     GLKEKYNTETVCLAFDIRDRSAMEKSLDSLPEEWKAIDILVNNAGLAAGLDPVNTAST
                     DDWDTMIDTNVKGLLYISRKVIPWMIERGKGHIFNIGSIAGKEVYPKGSVYCATKHAV
                     DALTKGMRIDLLPHGIKVTQVSPGAVETEFSLVRFKGDHDRAGMVYKGYEPLVAEDIA
                     AVIHFISSLPPRVNINDILVMPQAQANATTFNKM"
     gene            complement(5772..6638)
                     /locus_tag="PKZ74_06255"
     CDS             complement(5772..6638)
                     /locus_tag="PKZ74_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM92396.1"
                     /translation="MKTEYILTRRKEKLGSLTCKKFLLAMIISPLLAFTQGGYRITPD
                     VVYGHKAGMALTYDVFQPAGPANGAGLIHLVSGGWNSRYNPPDSVIEDYKPYLDSGFT
                     VFALRHGSSPTFKLPETVDDVLLGSWQIRRNAHRFGVDSLRLGIFGGSSGGHLSLMAG
                     LSGDRHPVSAIVAFFPPVDLRNIPDFMKAMFPAFDFDTTLAAGISPVLFASPDDPPTL
                     LIHGDKDFVVAPRQSERMIAALEENSVVSQLIVFEGIGHGNSYGAKGKYYEESTRETI
                     GWFRKYLVDPYK"
     gene            complement(6677..7567)
                     /locus_tag="PKZ74_06260"
     CDS             complement(6677..7567)
                     /locus_tag="PKZ74_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPM92397.1"
                     /translation="MKQKNHTLSRFQSIVLSLLIYIIAGITAVLIARLFTGHHPLVMI
                     GAGDLAATVVVFLFSVILNNSSAYDPYWSVKPIVIAAAYVFIFHLDDLTIRQLLVLAG
                     IFLYALRLTSNFYRDWPGFHHEDWRYVNFRKSSGKAYWVVSFLAIHLFPTIMVYLGCM
                     AMFAVFSGEGRALNIWDIPAALILFGSVVYVFVADEQLRNFRKDSSNKGKTIMSGLWR
                     LSRHPNYLGEISTWWGLALFAIAAGCHFWWTLAGPVAITLMFIFASIPMIEKRNLQRR
                     EGYTEYRKNTPMLLPLKFFR"
     gene            complement(7616..8539)
                     /locus_tag="PKZ74_06265"
     CDS             complement(7616..8539)
                     /locus_tag="PKZ74_06265"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPM92398.1"
                     /translation="MIRQAVIVLVSIIPTIIFSQNNMKYNKLTTEEEYIIIQKGTERP
                     WTGKYLNNKETGTYTCRRCDAPLYRSEDKFDSHCGWPSFDDEIKGAVKRIPDPDGMRT
                     EIICASCGGHLGHVFLDEGFTDKNTRHCVNSISMNFIPDSEAANQAVNRAIFAGGCFW
                     GVEYYMQKKPGVISVVSGYIGGHKDNPTYQEVCTGTTGHAEAVEITFDPSKVSYEELA
                     RLFFEIHDPTQVNRQGPDVGAQYRSEIFYLDENQKIITENLIGILKNKGFKVATKVTK
                     ATTFWPAENYHQDYYDNKGTLPYCHGYTKRF"
     gene            complement(8715..>10716)
                     /locus_tag="PKZ74_06270"
     CDS             complement(8715..>10716)
                     /locus_tag="PKZ74_06270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPM92399.1"
                     /translation="QLEAVACKDWLTNKVDRSVTGRIAKQQCAGTVQLPLNNLGVATM
                     DYLGKRGIATAIGHAPVAGLIDPEKGSVLAVAEALTNIVWAPLEDGLKGVSLSANWMW
                     PAKNPGEDARLFAAVKKVSEFAIGLGINIPTGKDSLSMTQKYPDGQKVFAPGTVIISA
                     VGEVANVRQIVEPVLQPAPPVETLHATPPVETLHATSLQGAGCIIYIDFSGDGFHLGG
                     SSFAQSVNAIGTAVPGVPDTGYFARAFETVQNMIREEMILAGHDISAGGMITTLLEMT
                     FGRNDAGMNLNMDAVGEKDIYRILFSEKPGVIIQVKDPDEASRLLEKEQIRFYRLGQV
                     TGNRTIEIGHDGQSYLFDIEELRDTWYKTSYLLDRKQCGPELAARRYENYKKQELSFR
                     FPDSFSGRFAQYGIEQARVVKSGIKAAIIREKGVNGDREMAWSMHLAGFDVKDVHMTD
                     LIAGRENLEDVNLIVFVGGFSNSDVLGSAKGWAGAFLYNPKAREALDKFYARHDTLSL
                     GVCNGCQVMVELGLVYPEHARMPKMLHNASGKFESAFLNVDVHETKSIMLKSLAGSRL
                     GIWVAHGEGQFSLPYEEGKYEIAMKYSYTGYPGNPNGSDYCAAALCSHDGRHLVMMPH
                     LERAIHPWNWASYPASRPSDEVSPWIEAFVNAKKWIETRCGD"
BASE COUNT         2689 a         2687 c         2578 g         2762 t
ORIGIN      
        1 cctgtcggag agaataaatg cactcatagc agtaacgaaa gatcccggta tgatttaatt
       61 ttccgggatc aactccttcc cgggactatt cccagttacc atcggtggaa ggttcttcca
      121 tcgacccgat cttcagaccg ggatactttt cgatatcttc ctttgatttg ataagattta
      181 taacaagctg acgatcaagg cctttgagga aggttgaata atgagctttt atctcataaa
      241 ctcccacttt aatcccggac ctgtcgattt cgctggcagc gatctcaaac aattctccgc
      301 cagagaaagg gatataacga agggaatcca ggttgtagcc cggcctggcg cggaacagcg
      361 agtcggctac tttcacataa ccgagcgtat cgttgatggt tttggtaaat gtggtatcat
      421 tcggatcggg gatcattttt accacgggga tttcaccgtt tctcagaaaa ctgataaggg
      481 tatcgaaact ggcggtgtat ttattatgtt gccttttata aaactgctga ctggtcctga
      541 gatcggtgag tgtggcgatt accttggctt cgcgagcgct ggtagctttg ttaaacctga
      601 cgggtctcaa tatgctttca tagataagat aagccagaac cacgatgata actgaaagga
      661 caaccttgat aatcgtgtat ttcatatcaa aaaagtttgg ttttattgaa aagcaaatgt
      721 ataaattatt ttctatctca aacggatatt tattgattaa aaataggcgt tttcttcctt
      781 ttcattcctt tacccggatt gctttaaatt ttacagaaac tggaattcag gaagatcatc
      841 cagaagtttc agggaacccg gatcattgcc aacctggcaa taaacatgct gaatcccgtt
      901 cgacaagacc aggtatttga ctttcagtgc caggttatac cgcaccgcct ggtcgagcac
      961 ggattgcgta acggcaactg caggcgactt gaattcggcc agcagcaggg cattgccgtt
     1021 acggtcgctg acaaccaggt cgaaacgctt tttagtcctg aacaacttga taacagcttc
     1081 gatgcggata agtgagacag gtacgttctt ataaccggta aggtaccgga tggcatgttg
     1141 cctgacccat tcctccggtg tcagagccac aaaccgcctt ctgacgggat caaagatcaa
     1201 ctcttttcca tcgcggattt cgcgtttgaa ttcaaattcg gggaaattca gttttatcat
     1261 tattttcggg gagttcttca gcacggaatt ccatctcaaa attaacaaaa atcctacctt
     1321 tgcaaaaacg gaacggatga agaccaaaga ggagattgtg aaaaactggc tgccgcgtta
     1381 tacgggccgg tcgctggaag atttcggaaa gtacatcctg ctaaccaact tcgatcatta
     1441 tgttgagttg tttgctaacc tccacaacgt tgagatcacg ggacttgacc gccccatgcc
     1501 caatgcgaca gccggcggaa taacgatcat caattttggt atggggagcg cgaatgccgc
     1561 aacgatcatg gatttgttgt cggccatcga acccaaagca atgctgttta ttggcaaatg
     1621 cggcggattg aagaaaaaga acgagatagg cgatctgatc cttcctatag cggccattcg
     1681 cggcgaaggg acctctaacg attattttcc tcccgaggtg ccggcattgc ccgcattcaa
     1741 cctgcagaaa gccgtttcga ctaccatccg cgaatataac cacgactatt ggacgggcac
     1801 ggtttacacc actaaccggc gggtatggga atatgacgag gagttcaagg agcggctccg
     1861 cagaatgaga tgtatggcga tcgatatgga gaccgctacc ctcttcatcg ccggctttgc
     1921 caacagcatt ccgtcgggcg ccctgctgct ggtcagcgac cagcccatga tccctgaagg
     1981 gattaaaacc gaggaaaccg acaggcaggt catccgtgac catgtggaaa cccaccttcg
     2041 gatcggtatc gattcactgc agttgttgat taacgacgga ctcactgtca agcacttacg
     2101 cttctgatca ctatgactta cgaccagatc acaggtgacc tccggcgaaa gatctaccgt
     2161 cccgtttacc ttcttcatgg agaagagccc tattttatcg atgccatcac cgcgatgatc
     2221 gaggagagcg ttctgaccga atccgaaaga gagttcaacc agaccattgt atatggacgt
     2281 gataccgatc cgggtaagat catcgacata gcccggcgct ttcctatgat ggccaattac
     2341 caggtagtga tcgtaaaaga agcgcaggat atcgataaaa tcgaaaacct gaagccctat
     2401 gtcgagaaac cggttccgac caccatcctg gtacttgcgc ataaacacaa gaaactcgac
     2461 cagcggaaag gcttgcacaa aatcattgaa aaaacgggag tattattcga gtcgaagaaa
     2521 ttatacgatg ataagattcc ggcgtggatc aacgggcagg tccaggccaa aggctacacg
     2581 atcaaaccgg atgcctgcca gttgctggcc gaatacctgg gcgccgacct aggccggatc
     2641 atcaatgagc tggaaaagct ggtcatcagc cttccaggcg gcaccgccat cgatgcggcc
     2701 ctgatcgaga ggaacatcgg catcagcaaa gattacaatt tcatcgagct tcagaatgct
     2761 atcggtaacc gcgatattca caaagctaac cgcattgtga accattttac ggccaacctg
     2821 aagcagaatc cgaccatcgc gctcgttgcc attttatatg gtttcttcat aaaactgatg
     2881 atttaccatc aattgaaaga taaatcccca aaaaacgctg catccgacat ggggatccac
     2941 cccttctttt ataaagatta tgaaaaagcg gcaaagaatt acagctttcg ccggataaga
     3001 cccatcatcg ctgtgttacg cgaatatgac ctgcggctta agggggtgaa caacggttct
     3061 acggaggagg cagggctgct cagggaattg gtttacaaga tcatgcactg atatctagcg
     3121 gaaaagcctg ctgacttctt ccttatattt atcgaggata tatgaccgct taagcttgag
     3181 gtttgccgtc agctcccctg tttcaaggct ccattcatgg tcgatcagtt cgaagctttg
     3241 aatctgctct gtgctgccaa agaatttgtt gaaatgatcg atctctttct tgaaccgttt
     3301 aaccaccagc ggatttttaa tcatctcttt atcagttgta taaggcatat tcttatgttc
     3361 gcaccacgat ttcaggtggt taaaatcggg aacgaccagc gctgctgcgt acttctgatt
     3421 ttcgcccagg ataaggatgc catcgataaa ggctgattct ttgaatttat tttcgagcaa
     3481 ctgggggctg atatatttcc ccagcgatgt tttaaagatt tcttttttcc ttccggtgat
     3541 ccgcaggtgt ccttcgggtt cgaggattcc cacatctccg gtatggaacc agccgtcggc
     3601 atcgatcgct tcagcagtca tacccggttc tttgaaatac ccctgcatga tattggctcc
     3661 acgggccagg atctccccat ccggtccaat tttcacctct gtgtttttca gaggtttacc
     3721 aaccgttccg aacattcggc caccctcttc cagcgtgttc acggccagca caggggaagt
     3781 ttccgtcagg ccataccctt cgagaacagg gatgccggca gcggtgtaaa cgcgggcaag
     3841 acggggctga agggcggcgc cgcccgaaac gatcacacgg acattcccgc caagggcctc
     3901 tctccactta acgaacacca atttattggc cagacggagc tgtgcatgat agagccagcc
     3961 tttcccttcg agttcataac ggttggccag gctcacagcc cagaaaaata tcgccttctt
     4021 aaatcccttt agctggctgc ctttagcaat gatcttatca tagacctttt cgagcagccg
     4081 gggaacggtc gtaaatatct cgggcctgat ctcgcggatg ttatcggcga tagtgcccat
     4141 attctcagcg taataaatcg aaagcccaag atagtgatag gtataaatgt ccatccgctc
     4201 atagacgtgg cacaacggca gataactgag gcctttgcag ttctcatcca ccggaaagat
     4261 gtgtttgacg cccatgacat tgctcaacag gttccggtgc gaaagcatga cccctttcgg
     4321 aaatcccgtg gtgccggaag tatatatcag ggtagccaga tcgtcagggt ttaccgcttc
     4381 ttttagtttt tccagttttt cagattggcg attggtttgg ccaagggcta tcaactcgtc
     4441 aaggtgtttg atcccctcaa tggctttaaa agtataaaca tcctgaacag cattaatttc
     4501 cggaagaatg tgctcgattt tccgaagaat atcccttccg gagataaata caaaactgac
     4561 ttcggagtgt ttgagaatgt atttgtaatc cgactcgctt atcgtcggat agatcggaac
     4621 gtgaacggcc ccgatctgta aaattgccat gtcaacgaaa ttccattccg ggcggttgtt
     4681 ggagatcgta gcgatcttat ctccgggccg caccccgagt ttcagcaacc catggctgat
     4741 attgttcaca ttctcaatat actggtcgat cgaatactta atccatttac caccatcttt
     4801 acctgccagt acatcatctt ttggtttaaa agaagtcttg taaaaaggaa gaagatcaaa
     4861 gagtctggtt acttccatgt ggtttgattt gggatctaaa aagcgcgcaa agatataaaa
     4921 atatttccta tatagtattt tatatatgag ctacttaaaa agaggcctaa aagtaataaa
     4981 tcttgaaatg aaaaaatcaa gaataaatca cattttatta aaagttgttg cattcgcctg
     5041 cgcctgtggc attacaagga tatcattgat gttgaccctc ggcggaagcg aggagataaa
     5101 atgtatgacc gcagcaatat cttctgccac cagcggttca tagcctttat aaaccatccc
     5161 ggcccggtcg tgatcgcctt tgaaacgcac cagggagaat tccgtttcca cagcgccggg
     5221 gctgacctgg gtcactttga tgccatgggg gagcaaatct atccgcatcc ctttggtgag
     5281 ggcatccacg gcatgcttgg tggcgcaata gacgctacct ttcgggtaca cttcttttcc
     5341 ggcgatggat ccaatgttga aaatatgccc cttgcccctt tcgatcatcc aggggatcac
     5401 tttacgggaa atatacagca aacccttcac attggtatcg atcatggtat cccagtcgtc
     5461 ggtcgaagcc gtgttcaccg gatccagccc ggcagccagg ccggcattat tgaccaggat
     5521 atcgatcgct ttccattctt cggggaggct atcgagactc ttctccatag ctgatctatc
     5581 gcggatatca aatgccaggc aaacagtttc ggtgttgtat ttttccttga gcccggtggc
     5641 aatatcctgc aggattttta ttctccggcc cgtgatgatc aggcgatggc cctcctgggc
     5701 aaaccggaat gcagttgctt ttcctatccc agccgttgcg ccggtaatga gtatggtttt
     5761 tatcatgagg tttatttata tggatctacg agatatttcc ggaaccagcc gatggtttcc
     5821 ctggtgcttt cttcataata ttttcctttg gcgccgtagc tgttgccgtg tcctatgcct
     5881 tcgaagacga tcagttgcga aaccacgcta ttttcctcca gcgcagcgat catccgttcc
     5941 gattgcctgg gcgcaacgac aaaatcttta tcaccgtgta tcagcaatgt cggagggtca
     6001 tcagggctgg cgaaaagcac gggagatatt ccggcggcca gggtggtatc aaaatcgaat
     6061 gcggggaaca ttgctttcat gaaatcgggg atattcctca ggtcgaccgg cgggaagaaa
     6121 gcgacaatag cgctgaccgg gtgcctgtcg cccgaaaggc cggccattag tgaaagatgg
     6181 ccgccggagc tgcctccgaa aatgcccagc ctcagtgaat cgacgccaaa acggtgcgca
     6241 ttcctgcgga tctgccatga tccaaggaga acgtcgtcga cggtctcagg gagcttgaaa
     6301 gtcgggctgc tgccatggcg caaggcaaaa acggtaaatc ccgagtcgag gtaaggcttg
     6361 taatcttcaa tgactgagtc gggcgggtta tagcgggaat tccatccccc gctgaccagg
     6421 tggatgaggc ctgctccgtt ggccggtccg gccggctgga agacatcgta ggttaacgcc
     6481 attcccgctt tatggccata aaccacatcg ggtgtaatac ggtacccacc ctgggtaaag
     6541 gccagaagcg ggctaataat catggcaaga agaaacttct tacaggtcag ggagccgagc
     6601 ttttccttcc gacgggttaa tatatattca gttttcatgg cggtataaca aacagaaatg
     6661 gatgtttgtt aaaggattat ctgaaaaatt tcaatggcag caacatcggt gtgttcttcc
     6721 ggtattccgt gtatccttcc ctgcgctgaa ggttgcgttt ttcaatcatc gggatgctgg
     6781 cgaaaatgaa catcagggtt atggcgaccg ggccggccag tgtccaccag aaatggcagc
     6841 ctgcggcaat ggcaaataaa gccagccccc accaggtact gatctctccc aggtaattgg
     6901 gatggcggct taagcgccac aggccggaca tgatcgtttt tcccttgttt gaactgtctt
     6961 ttctgaagtt cctgagctgt tcatccgcaa caaatacata aaccacgctt ccaaaaagga
     7021 tcagggcggc cgggatatcc cagatattca gtgcccttcc ttcaccggaa aatacggcaa
     7081 acatagccat gcacccaagg tagaccataa tggtcgggaa caggtggatt gccaggaaac
     7141 tcaccaccca gtatgctttt ccgctggatt tcctgaaatt tacatagcgc cagtcttcat
     7201 gatggaaccc gggccagtcg cggtaaaaat tcgaagtaag acgaagggca tacaggaata
     7261 tgccggcaag aaccagcaac tgcctgatgg tcaggtcatc aagatgaaaa ataaaaacat
     7321 aagctgccgc gatcacaata ggttttacac tccaataggg atcataggcg gaggaattgt
     7381 tcaggatgac cgaaaacaga aagacgacca cggtggctgc caggtctcct gccccaatca
     7441 tgaccaaggg atgatgaccg gtgaataacc ttgcaataag cactgcggtt atgccggcga
     7501 taatgtagat caacaggctg agcacaatgc tctgaaacct gcttagagta tgatttttct
     7561 gtttcatttt taattcattt gccattcagg cacctaaact ccaattcatt aaaaactaga
     7621 atctcttcgt atatccatgg caatagggca aggtgccttt attgtcgtaa taatcctggt
     7681 ggtaattttc tgccggccag aaggttgtcg cttttgtgac tttcgtggcc actttgaagc
     7741 ctttgttttt gaggattccg ataaggtttt cagtgattat cttctgattt tcatccaggt
     7801 aaaaaatttc ggaacggtac tgtgcgccga catcgggacc ctggcggttg acctgggtcg
     7861 ggtcgtggat ttcgaagaag aggcgggcca gttcttcgta agacacttta gaaggatcga
     7921 aggtaatctc tactgcttcg gcatggccgg tggttcccgt gcagacctcc tggtaggtag
     7981 gattatcttt atgtccgccg atataacccg aaacgaccga gatcacaccc ggtttcttct
     8041 gcatatagta ttcgacgccc caaaagcatc ctccggcgaa tattgcccgg tttacggcct
     8101 gattggccgc ttctgaatca ggaataaaat tcatagagat ggaatttacg caatgacgtg
     8161 tgtttttatc tgtaaagcct tcatccagaa agacgtgccc caggtgtccg ccgcaactgg
     8221 cgcagatgat ctctgtccgc ataccatcgg gatcgggaat ccttttgact gcccctttga
     8281 tctcatcgtc gaagctgggc catccgcagt gggagtcgaa cttatcttcc gacctgtaaa
     8341 gcggggcgtc acaccggcgg caggtataag tacctgtttc tttattgttc aggtattttc
     8401 cagtccacgg gcgctcagtc cctttttgga tgatgatata ttcttcctcc gtggttaact
     8461 tattgtattt catattgttt tgcgagaata taatggttgg gatgatggag actaaaacga
     8521 ttacagcttg ccggatcatt atcatcaatt ttaacaccta actgttttgt gccaattttg
     8581 tttaaaaatt tgttgatccc ctccggggaa aaattacaac acgctcattt ttctataaga
     8641 attgatcccc tacggggaat ataatacatc gatgatcgcc tgttcgcata atcgcctgtt
     8701 cgcccgtacg cttgttaatc cccgcatctc gtttcgatcc atttttttgc gttgacgaat
     8761 gcttcgatcc agggtgagac ctcatcggaa gggcggctgg cgggatagct ggcccagttc
     8821 cagggatgga tggcccgctc caggtggggc atcatgacca ggtggcggcc atcgtgcgaa
     8881 caaagcgcag cagcacagta atcgcttccg ttaggattgc cgggatagcc ggtataactg
     8941 tatttcatcg cgatctcata tttgccttcc tcatacggca gggagaactg cccttctcca
     9001 tgcgcaaccc agatgcccag ccggctgccg gcaaggcttt tcaacataat gcttttggtc
     9061 tcatgaacat ccacattaag aaaagccgat tcgaacttac ccgaagcatt gtgcagcatc
     9121 ttcggcatgc gggcatgctc aggatagacc agtcccagtt cgaccattac ctggcatccg
     9181 ttacagacgc caaggctaag ggtatcatga cgggcgtaga acttatcaag cgcttccctg
     9241 gctttaggat tgtaaaggaa agcaccggcc cagcccttcg ccgagcccaa aacatcgctg
     9301 ttggagaatc ctccgacaaa cacgatgaga ttaacatctt ccaggttctc gcggccggcg
     9361 ataaggtcgg tcatatgaac gtcttttaca tcgaacccgg ccaggtgcat gctccaggcc
     9421 atctcccggt cgccgttcac ccctttctcg cggataatcg cggctttgat gcccgacttt
     9481 acaacccttg cctgctcaat cccatattga gcaaatctgc cgctgaaaga atcaggaaac
     9541 cggaacgaca actcctgctt tttgtaattt tcgtatcttc tggcagctag ttccggtcca
     9601 cactgcttac gatcaagcag ataggatgtt ttgtaccatg tatccctgag ttcctcaata
     9661 tcaaacaaat aggattgtcc atcatggccg atttcaatcg tacggttgcc ggtaacctga
     9721 ccgagacgat aaaatctgat ctgctctttt tcaagtaacc gtgatgcttc gtccgggtct
     9781 ttcacctgga tgatcacacc gggtttttca ctgaaaagga tgcggtagat gtccttctcg
     9841 ccgacagcat ccatgtttag attcatcccg gcatcattcc ggccgaaagt catctccagc
     9901 agagtggtaa tcataccgcc ggcgctgata tcatgtccag ccaggatcat ctcttcacgt
     9961 atcatattct gcacggtttc gaatgcccgc gcaaaatatc cggtatcagg cacgcccgga
    10021 actgcggtgc cgatggcgtt aaccgattgg gcgaaactgc tgccccccag gtggaatccg
    10081 tcgcccgaaa aatcgatata aatgatgcaa ccggccccct gtagagacgt tgcatgcaac
    10141 gtctctacag ggggcgttgc atgcaacgtc tctacaggcg gcgccggttg caacaccggt
    10201 tccacgatct gccggacatt tgcgacctcc cctacggcgg aaataatgac ggtacccggg
    10261 gcaaaaacct tttgtccgtc agggtatttc tgtgtcatcg acaatgaatc tttgccggtg
    10321 ggtatattga tcccgaggcc gatggcaaat tcgctgacct ttttaacggc agcaaacagc
    10381 ctggcgtctt cgcccggatt ttttgccggc cacatccagt tagcgctgag ggatacgcct
    10441 ttcaggccat cttccagcgg cgcccagaca atattcgtca gagcttccgc aacggccagc
    10501 acagatcctt tctccggatc gatcagtccg gcgactggcg catggccgat cgcagtagcg
    10561 attccccttt ttccaaggta atccatcgtc gctaccccca ggttgttcaa aggcagttgc
    10621 accgttccgg cacattgctg tttggctatc ctgcccgtca ccgaccggtc gaccttgttg
    10681 gtcagccagt ctttgcaggc gacggcttct agttgc
//
LOCUS       DAOPNH010000091        10588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6737_length_10588_cov_6.378715, whole genome shotgun sequence.
ACCESSION   DAOPNH010000091 DAOPNH010000000
VERSION     DAOPNH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6737_length_10588_cov_6.378715"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKZ74_06275"
     CDS             complement(<1..803)
                     /locus_tag="PKZ74_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPM92400.1"
                     /translation="MKIPLNQFEQYIDETILKRGLSYFKKGLVEDPEELSPGFFEAIV
                     EGSESYRVTVSIKNEIITDHSCTCPYDLGPVCKHIAALLFALQAEKLGINKQNKITAA
                     KNSTEMTRKKGRKTVIEQVDEILTKLPPNHIAEFIREQCIQNPAFRRLFIARFITLFE
                     GESKAVYAQQVKAILNSAKGRHGFIEWNRAGAVGKAVYEMLVNARKQLEVKNYQTVML
                     ISCAVLEEMFKALQFADDSDGDIGGGVSEAVEILFLVSEEDLPEENRKWL"
     gene            complement(821..1183)
                     /locus_tag="PKZ74_06280"
     CDS             complement(821..1183)
                     /locus_tag="PKZ74_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HPM92401.1"
                     /translation="MKSLTGSATLLRIYVGELDKYHHQPLYEAIVLFAKRQGLAGATV
                     LRGLMSYGANSMIHTSKLFALSEDLPIIIEIVDEEDKIQDFIQKLQPYFESAKYGGLV
                     TLEEIRVIYYQNSKTTQD"
     gene            complement(1186..1563)
                     /gene="crcB"
                     /locus_tag="PKZ74_06285"
     CDS             complement(1186..1563)
                     /gene="crcB"
                     /locus_tag="PKZ74_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPM92402.1"
                     /translation="MLKMFLITGAGGFIGSGLRYLVQRIIAIYLPVTFPFATLFINVF
                     GSLLIGIIYALSDKTSMLNPEMRIFLAIGVCGGFTTFSTFSLDTYSLVKDGAYLFVSL
                     YIFASVILSIAAVFAGIWIVKSL"
     regulatory      complement(1579..1640)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1646..1915)
                     /locus_tag="PKZ74_06290"
     CDS             complement(1646..1915)
                     /locus_tag="PKZ74_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92403.1"
                     /translation="MNEISAMKLDVISGGGRKKDFWDIHELLNHFSIQQMLDLHEKRY
                     PYSHDPSQILKKLTDFNSADDDFNPFCLKGKHWELIKLDLIEWVK"
     gene            complement(1912..2112)
                     /locus_tag="PKZ74_06295"
     CDS             complement(1912..2112)
                     /locus_tag="PKZ74_06295"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPM92404.1"
                     /translation="MAIHKLFYNTTTPLLLEILKALMVSDEFRPFRLVGGTALSLQLG
                     HRKSLDIDLFTDRDYDFIDFQA"
     gene            complement(2099..2560)
                     /locus_tag="PKZ74_06300"
     CDS             complement(2099..2560)
                     /locus_tag="PKZ74_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance system antidote protein"
                     /protein_id="HPM92405.1"
                     /translation="MNNELTIIKGIHPGFYLERELRRRNLGKSKFAISVGEYPQTLVS
                     ITKGKRKMNTSLALKIEKELGLEEGYLMILQIYHDIEKEKMNLNLSRPDLSKIRPVLF
                     WDTEPEKINWILQKEAVIKRVFERGNEEEKEELIRFYGIDAVNEVIKNGNS"
     gene            2746..3159
                     /locus_tag="PKZ74_06305"
     CDS             2746..3159
                     /locus_tag="PKZ74_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPM92406.1"
                     /translation="MASKTSKVIILNKRDFKAKIFDYNYSKEWEYKGDMPAVIDFYAD
                     WCQPCKVVSPLLDQLSMEYDGQVKFYKINTEKDPEVSKVFGISSIPTLLFIAREGRPV
                     VVRGAQPIGALRKNIERILPGEKKNFLQGLFSFKK"
     gene            3284..3676
                     /locus_tag="PKZ74_06310"
     CDS             3284..3676
                     /locus_tag="PKZ74_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92407.1"
                     /translation="MRKLSGIILTMALTAFFAVQVMAQTATPAATDDKPVKQTATTAQ
                     TCGKFVDNNNDGVCDNRGTQCKQGKGTGFTDANGDGICDHRSDGNCCKGNPDCCKGQK
                     SGCGKGYGPKHRQGCGGPCGARTVPDKK"
     gene            3769..4365
                     /locus_tag="PKZ74_06315"
     CDS             3769..4365
                     /locus_tag="PKZ74_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92408.1"
                     /translation="MTEQELISKANGRDREALSQLVETYQRKIIKTAYYYLGNMEDAE
                     DLSQEIFVKILTSLPRFRQSSSLSTWIYRIAVNESLNAVRRNRQRQIFCRIERIFGIT
                     ENGSKGIRREIQAETDTLEAEYQRQWLWEAISSLPEKQRTVFILHKMEELPYAEISSI
                     TGQTLSSVESLMHRARLNLQKKLLKPDITNIKTTSHEL"
     gene            4355..4783
                     /locus_tag="PKZ74_06320"
     CDS             4355..4783
                     /locus_tag="PKZ74_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92409.1"
                     /translation="MNCKEFRSFLILSQDQALNPKQLPGMEDHARDCRSCKMLLHKQE
                     LLEHFITAEQVREPNPFVSTRILQRLTGMQAPVTAGKSLVIRPVLVTLGLLAALAAGF
                     LIGTAGSVQEPGLTEENRIETLRTGLFVSDFADEDINLIE"
     gene            4795..5301
                     /locus_tag="PKZ74_06325"
     CDS             4795..5301
                     /locus_tag="PKZ74_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPM92410.1"
                     /translation="METITRNRVLFWLLIFLIIVNLAALATYFILPEKKQPAVCAGDP
                     NSPECILHSRLNLTDEQNEHVERINNEYRVVSQPLSQEIKKLRGIILDELETGTPDTA
                     LIHNLTGEIAALQLQLHRENILHYLDLKEVCNPEQALLLSNLYREMYGCPMHREMGQP
                     KHHRYRGH"
     gene            complement(5354..6892)
                     /locus_tag="PKZ74_06330"
     CDS             complement(5354..6892)
                     /locus_tag="PKZ74_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPM92411.1"
                     /translation="MRKEVLERLGISAINDGVCTGYDWHGGGGAVSVSVSPIDNKEIG
                     KVRNASPEDYEFVMQKAQNAFLEWRNVPAPKRGEVVRQIGDALRASKEDLAYLVTLEM
                     GKIYQEGLGEVQEMIDICDFAVGQARLLNGFTMHSERKDHRMYDQYHPLGIVGLITSF
                     NFPVAVWAWNSMIAAIAGDVVVWKPSSKTPLTAIATQNIIREVLKKNNVPEGVFNLLV
                     AGSNVLGDNFVADRRIPLFSVTGSTAVGKRVGEIVGKRLGRTILELGGNNAVIVSHSA
                     NLEMAIKSIVFGAVGTAGQRCTSTRRVIIHEAVYDAVKDKLVLAYNSVLKKIGDPLDK
                     ETLMGPLIDGKAVGNFLHALDQAKAEGGKVIFGGKVLEGEKYVSGNYITPALVEAENH
                     YKIVQEEIFAPILYLIKYRWVDEAIALNNGVPQGLSSAIFTENLREAEMFLSEAGSDC
                     GIANVNIGTSGAEIGGAFGGEKDTGGGRESGSDSWKLYMRRQTNTINYSTELPLAQGI
                     EFGF"
     gene            complement(6941..8569)
                     /gene="pruA"
                     /locus_tag="PKZ74_06335"
     CDS             complement(6941..8569)
                     /gene="pruA"
                     /locus_tag="PKZ74_06335"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPM92412.1"
                     /translation="MNNALYNFPAPQNEPVSGYLSNSPERIALEQELHRQSLEKVEIP
                     LIIGGKEIRTGNTAELRMPHDHSKVIGIYHMVGEKEIKVAIEAALKAHQTWSNLAWTI
                     RASILLKAAELIATKYRALMNAATMLGQSKNIFQAEIDSVCETVDFLKYNSYFAGRIY
                     EIQPRSSFNQLNRMEYRALEGFILTISPFNFTSIASNLNMAPVMMGNTTLWKPATTSL
                     LSNYYLMKIFLEAGLPEGVINFIPGSGSLIGKTVLDSPEMAGIHFTGSNNTFNLLWGQ
                     VSANLASYRSYPRLVGETGGKDFIFVHPSADPLEVAVNAIRGAFEYQGQKCSAASRMY
                     VPQSLWPEIKPMLIDLANDIKIGDVRDAGNFMNAVIDKGSFETIKGYIDFAKVSAEAK
                     ILAGGDCDDSVGYFIRPTLVEVSDPKHKLMAEEIFGPVLTIFVYKDADLDETIELCNT
                     TSPYGLTGAVFSRDRVAASRICEKLQYAAGNFYINDKPTGAIVGLQPFGGSRASGTND
                     KAGGEFNLVRWISPRTIKETFVPATNYKYPYMME"
     gene            8727..10373
                     /locus_tag="PKZ74_06340"
     CDS             8727..10373
                     /locus_tag="PKZ74_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM92413.1"
                     /translation="MQTSRKSAMEDRRRIIIYLPVILAFVLILGIWLGSKMNFSKSGE
                     TQNPIFNRAVQHDKITDLINYVEQDYVDSVSREDLTVNAIEGVLDKLDPHSTYIPAED
                     FGEAEDQLDDNFEGIGIQFRIEQDTIMVIQVIPGGPSEKVGLMAGDRIVMINDSLVAG
                     TGLTSRDAVKQLKGKRGTVVKVGILRRGLAELVDFSITRDVIPTYSIDISYMVNDSVG
                     YVKLSRFAVSTHEEMIRAMSGLLENGMKRLILDLRDNTGGYLQASIHVSDEFLGDKKL
                     IVYTEGNSRPNQYAYATKRGVFEDYPLVILINEASASASEIVAGAIQDNDRGLIVGRR
                     SFGKGLVQEQLTFPDGSAVRLTVARYHTPTGRSIQRPYKEGEKDEYYHELYDRYANGE
                     MENPDSISLTDTVKYYTPAGKVVYGGGGIMPDVYVALAAEEKEVLYRKLIHQNTIFQY
                     AFNYTDRERPSLAKYSTPKEFIERFKVDDKLFSDLMDYARNQDIYYTPSELIDSKEKI
                     KTLLKAYIGRNLLDDEAFYPVYHEVDKVFQIALDTLIKNN"
     gene            10487..>10588
                     /locus_tag="PKZ74_06345"
     CDS             10487..>10588
                     /locus_tag="PKZ74_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655997.1"
                     /note="SodB; iron binding; present under aerobic and
                     anaerobic conditions; destroys free radicals; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HPM92414.1"
                     /translation="MNFELPSLPYALDALEPFISKNTLEFHYGKHHQA"
BASE COUNT         2785 a         2624 c         2424 g         2755 t
ORIGIN      
        1 agccatttcc ggttctcttc aggcaggtct tcctctgaaa caagaaacag gatctccaca
       61 gcttcgctga cacctcctcc gatatcgccg tcgctgtcat ccgcaaattg aagcgctttg
      121 aacatttcct ccagaacagc gcaggaaatg agcatgaccg tctgatagtt tttcacttcc
      181 aactgtttcc gggcattgac cagcatttca tatacagctt ttcctaccgc tccggccctg
      241 ttccattcta taaatccatg acgccctttt gccgaattaa gtatcgcctt tacctgttga
      301 gcgtaaaccg ctttcgattc accctcaaac agggtaataa accgggcaat aaacagcctg
      361 cgaaatgcag gattctgtat gcactgttcc cgtataaatt ccgcaatatg atttggaggc
      421 agtttggtca ggatctcatc tacctgctca atgaccgttt tccgaccttt ctttctggtc
      481 atttctgttg aatttttagc agcggttatt ttattttgct tgttaattcc aagcttttca
      541 gcctgtaaag caaataagag cgctgcaata tgcttgcaca ccggacccag atcgtatggg
      601 caggtacaag aatggtccgt aatgatttcg tttttaatag aaaccgttac cctgtatgat
      661 tcagatcctt caactatagc ttcgaagaat cccggcgata attcctcagg atcctccacc
      721 aggcctttct tgaaataact taaacctctt ttcaggatag tttcatcgat atactgctca
      781 aactggttta aagggatttt catgaggttc gcttttatgt ttaatcctga gttgttttac
      841 tgttctgata ataaatcacc ctgatctctt ccagtgtcac cagcccgccg tactttgcac
      901 tttcgaaata gggttgaagc ttttggatga aatcctgtat tttatcctct tcatccacga
      961 tttcgatgat gatgggtaga tcttccgaaa gggcaaataa ttttgaagta tggatcatgc
     1021 tgttcgcccc gtacgacata agcccgcgca atacggtggc gccggccagc ccctgtcgtt
     1081 tggcaaacaa gacaatggct tcgtaaagcg gctgatgatg atacttatcg agttctccaa
     1141 cataaatgcg caataaagtg gcacttccgg taagtgattt catgactaca acgatttaac
     1201 gatccagatt ccggcgaaaa cggcggcaat acttaaaatt acactggcaa aaatatagag
     1261 ggatacaaat aaataggcac cgtctttgac caggctgtaa gtgtcgagcg aaaaggtcga
     1321 aaaagtggtg aatccgccgc aaacacctat agccagaaaa atccgcattt cgggatttaa
     1381 catgcttgtt ttgtcgctca gggcatatat tatcccgatg agcagggaac cgaaaacatt
     1441 gatgaacaag gtggcgaaag ggaaggtgac cgggagatag atggcgataa ttcgctgtac
     1501 caggtacctg agcccgcttc ctataaaacc tcctgcccct gtgattaaaa acatcttcaa
     1561 cataggaatt cgtttagttc aagaacagga gtcatcagct cgtgaggcgg ttcagggcga
     1621 actccatcgc cagcggcaaa aatacttatt taacccattc aatcaaatcc agcttgatta
     1681 attcccagtg tttgcctttc aggcagaacg gattaaagtc atcatcagcg ctgttgaaat
     1741 cagtcagctt ttttaatatt tgggacggat catgactata aggatacctt ttctcatgta
     1801 agtcaagcat ttgttgaatg ctgaagtgat tcaacaattc atggatatcc cagaaatcct
     1861 tttttctacc gcctccggat ataacatcta atttcattgc tgatatctca ttcatgcctg
     1921 aaagtcgatg aaatcgtaat ccctatctgt aaacaggtct atatctaatg atttccggtg
     1981 tccaagctgc aaacttaatg cggttcctcc gaccagcctg aagggcctga actcatcgct
     2041 taccatcaaa gccttcagga tttcaagcag aagaggtgtt gtagtattat agaacaattt
     2101 atgaattgcc attctttata acttcattga cagcatcaat cccgtaaaaa cgaattaatt
     2161 cttctttttc ttcctcattt cctctttcaa aaacccgttt aattaccgct tctttctgca
     2221 atatccagtt aattttttcc ggttctgtat cccaaaacaa aaccggcctt atttttgaaa
     2281 gatcggggcg tgatagattc aggttcattt tttctttttc aatgtcatga taaatctgaa
     2341 gaatcattaa atagccttct tcaaggccta gttccttttc aattttcaat gccagggaag
     2401 tattcatttt acgttttcct ttggtgatgg aaaccaaagt ctgtggatat tccccgacgg
     2461 aaattgcaaa tttgctctta cccagatttc gccgtctgag ttcacgttca aggtaaaatc
     2521 ccggatggat acctttaata attgtcaatt cattattcat cctgcaaaaa taaacataat
     2581 aatgtaaaca taaacgttta tttattcaat gattgatcac ccctggtatt ttctatttcg
     2641 caaaactatt attagtgata ttaaagcgat ttatttctgc ttttttatta cttttgccac
     2701 caatgcacga attcacgaaa ttcaatcatc ttaaatcatg ataacatggc ttcaaaaacc
     2761 agtaaagtaa tcatcctcaa caaaagggat tttaaagcga aaattttcga ttataactat
     2821 tccaaggaat gggaatacaa gggggatatg ccggccgtaa tcgactttta tgccgactgg
     2881 tgccagccat gcaaggtggt ttcccctctg ctcgaccagc tttcgatgga atacgacgga
     2941 caggtgaagt tttacaagat caacaccgaa aaagatccgg aagtctccaa agtgttcggc
     3001 atttccagta tcccaacatt gttgttcatt gcccgtgaag ggaggccggt ggtggtgagg
     3061 ggagcacaac ccatcggggc gctgcggaaa aatattgaaa ggattctgcc gggcgaaaaa
     3121 aagaacttct tacagggttt attttccttt aaaaagtaag gctttatgca agcatccggc
     3181 ggttgattat atgcatccct gacgcattga attaaaaatt ttcatacgac cgcaagtttt
     3241 ttgattttag gttgtctaaa aggaaaaatc aaattttaaa agtatgagaa aattatcagg
     3301 aattatttta acgatggcgc tgaccgcctt ttttgcagtc caggtaatgg cccagacggc
     3361 tacgcctgcc gccactgacg acaagccggt gaagcaaacc gccaccacgg cccaaacctg
     3421 cgggaagttt gttgataaca acaacgacgg agtctgcgat aaccgcggta cccagtgcaa
     3481 acaaggcaag ggaacaggat ttaccgatgc caacggcgac ggcatttgcg accaccgcag
     3541 cgatggaaat tgctgcaaag ggaacccgga ttgctgcaaa ggccagaaga gcggatgcgg
     3601 caaagggtat ggccctaaac accgccaggg ttgcggcgga ccttgcggag cccggaccgt
     3661 gccggacaaa aaataaagcg taattttatc gaaccgtcgg gtgcccggtt gtcaataatg
     3721 gataaccggg cgcctgaatt gttgaggtgt attctaactg aacttcggat gaccgaacag
     3781 gaattgatca gcaaagcgaa tggccgcgac cgcgaagccc tgtcacaact ggtcgaaacc
     3841 taccaaagaa aaattatcaa gactgcgtac tattaccttg gcaacatgga ggatgcggag
     3901 gacctgtcgc aggaaatctt tgtaaaaatc cttacttcgc taccccggtt ccgccaatca
     3961 tcgtcacttt ctacctggat ttaccggatc gctgtgaatg aatcattaaa tgccgtcagg
     4021 agaaacaggc aaagacagat tttctgcaga attgaaagga tattcgggat cactgaaaat
     4081 ggaagcaaag gcataagacg ggagatccag gcagaaactg acaccttaga ggctgaatat
     4141 cagagacaat ggttatggga agccatttct tctttaccgg aaaaacaacg gactgttttt
     4201 attttacata aaatggaaga attgccttat gcagaaatat cctctattac cggccagacc
     4261 ctgtcgtcgg tcgaatcgct gatgcaccgg gccaggctga atctccaaaa aaaactgctg
     4321 aagccggata tcacaaacat taaaactaca agccatgaac tgtaaggaat ttcggagttt
     4381 cctgattcta tctcaggacc aggcgctcaa tccgaagcaa ctgcccggca tggaagacca
     4441 tgcccgggat tgccgatcct gcaaaatgct gctgcataag caggaactcc tggaacattt
     4501 tatcacagct gagcaagtcc gggagcccaa tccctttgta tcgacacgga tcttgcaaag
     4561 gctgaccgga atgcaggcgc cggtcacagc cgggaaatca ctggtgatca ggcccgttct
     4621 ggttaccctt gggctattgg ccgccctggc cgccggattc ctgatcggta ccgccgggtc
     4681 agtccaggaa ccgggcttaa cggaagagaa ccggatcgaa acactgcgta caggcctatt
     4741 tgtcagcgac tttgccgacg aagacatcaa tctgatcgaa taaaatccaa tcctatggaa
     4801 accataacac gaaaccgcgt acttttctgg ctgctcatct ttttgatcat agtcaacctg
     4861 gcagccctgg caacttactt tattttgcct gagaaaaagc aacctgccgt atgcgccggc
     4921 gatcccaatt cacccgaatg tatcctgcat tccaggctca atcttaccga tgagcagaat
     4981 gagcatgttg aaaggataaa caacgaatac cgggttgttt cgcagcctct gtcacaggaa
     5041 atcaagaaat tacggggcat catcctggat gaacttgaaa ccggaacgcc cgatacagcc
     5101 cttatccata atctgaccgg tgagattgcg gcgcttcagc tacagcttca tcgtgagaat
     5161 atccttcatt atcttgatct gaaagaggtc tgcaatccgg aacaagccct gctcctctcc
     5221 aatctttacc gggagatgta cggttgcccc atgcaccggg aaatgggcca accaaagcat
     5281 caccggtaca ggggacattg aatgtaagaa gggttcccgg ggttgcaaat ccacccctgc
     5341 accccttacc acctcagaat ccaaactcaa tcccctgcgc cagaggcagt tcggtactgt
     5401 aattgatcgt attggtctgt cttctcatgt aaagtttcca gctatcggag cccgattcgc
     5461 gcccgccgcc ggtatctttc tcgccgccga atgcgccgcc gatctcagcg cccgaggtgc
     5521 cgatgttcac attggcgatg ccgcaatcgc tgccggcttc ggagaggaac atctccgcct
     5581 cgcgtaagtt ctcggtgaaa atggcagagg atagcccctg cggaacgccg ttgttaagcg
     5641 cgatcgcctc atcaacccaa cggtatttga tcaggtaaag gatcggggca aagatctcct
     5701 cctgaacgat cttataatgg ttctccgctt cgaccagggc cggcgtgatg taattcccgg
     5761 atacatactt ttcgccttcc agcacttttc ctccgaaaat tacctttccg ccctcggctt
     5821 tggcttgatc aagcgcatgc aggaagtttc cgactgcttt accgtcgatc agcgggccca
     5881 tcaacgtctc tttatccagc ggatccccga tctttttgag gacgctgttg taggccagca
     5941 ccagcttgtc tttcaccgca tcgtatacgg cttcgtggat gatcaccctg cgggtggagg
     6001 tgcagcgctg tccggccgtt cctacggcgc caaaaacgat cgacttgatc gccatctcca
     6061 ggttggccga atgtgatacg atcactgcgt tgttgccgcc gagctcgagg attgtccggc
     6121 ccaggcgttt gcccacgatc tcaccgactc tttttccaac agcagtcgat ccggtcaccg
     6181 aaaacagggg gattcgcctg tcggccacga aattatcgcc caggacattc gacccggcaa
     6241 ccagcaggtt gaagacacct tcggggacat tgtttttctt cagcacctcc cggatgatat
     6301 tttgtgtggc gatggccgtt aacggggttt ttgagctcgg tttccagacg accacatcgc
     6361 cggcaatggc agcgatcatc gaattccagg cccatacggc cacagggaag ttaaagctgg
     6421 tgatgagtcc gacaataccg agcgggtggt actggtcgta catccggtgg tctttccgtt
     6481 cggagtgcat cgtgaaaccg ttcagcaaac gggcctgccc cacggcaaaa tcgcagatat
     6541 cgatcatctc ctgcacttcg ccaagccctt cctggtatat tttacccatt tccagcgtaa
     6601 ccaggtaggc caggtcctct ttggaggccc tcagtgcatc cccgatctgg cgcaccacct
     6661 caccgcgttt tggcgccggc acgtttcgcc actccaggaa ggcattctgt gctttttgca
     6721 tcacaaattc ataatcttcc ggtgaagcat tccttacttt gcctatctct ttattatcaa
     6781 tcggggaaac agaaacggag accgctcccc cgcctccatg ccagtcataa cccgtgcata
     6841 cgccatcgtt tatggctgaa attcccaaac gctcaagtac ttcttttctc atgttcattt
     6901 tattaaatct tgataataaa cccttctgaa ggaaaattgt ttattccatc atataaggat
     6961 atttgtaatt tgtggctggt acaaaggtct ccttgatcgt tcgcggactg atccaccgga
     7021 cgagattaaa ctcgccgccg gctttatcat tggtccccga agcccttgat ccgccgaaag
     7081 gctgaagacc tacaatggcc ccggtaggct tgtcgttgat gtagaaatta ccggcggcat
     7141 actgaagctt ctcgcaaatc ctcgatgcgg ccacacggtc gcgggaaaac acggctcccg
     7201 tcagcccgta aggagaggtg gtgttgcaca attcaatagt ttcatccaga tcggcatcct
     7261 tataaacaaa aatagtcagt accgggccga agatctcttc tgccatcagt ttgtgcttcg
     7321 gatctgaaac ctcaacaagg gtcggacgaa taaaatagcc caccgaatca tcgcagtcac
     7381 cgccggcaag gatctttgct tcggccgata cttttgcaaa gtcgatatat cctttgatcg
     7441 tttcgaatga gcctttgtcg atcacggcat tcataaagtt accggcatcc ctgacatcgc
     7501 caattttaat atcgttggcc aggtcgatga gcatcggctt gatctcaggc cacaggcttt
     7561 gcggcacata catcctggag gcagccgagc atttttgtcc ctggtattca aaagcacccc
     7621 ggatggcatt gacggctact tccagcggat cggcggatgg atgaacgaaa atgaaatctt
     7681 tgccaccggt ctccccgacc agcctggggt aggatctgta gctggcaaga ttggccgaaa
     7741 cctgccccca gagcagattg aacgtattat tggaaccggt aaaatgaatc cctgccattt
     7801 cgggcgagtc caggaccgtt tttccgatca ggcttccact gccgggaatg aagttgatga
     7861 caccttccgg caaaccggcc tcgaggaata tcttcatcag gtaataattg gagaggagcg
     7921 aagtggtcgc cggtttccag agagtcgtat tgcccatcat aactggcgcc atgttcagat
     7981 tggaggcaat ggaagtaaag ttgaacgggc tgatggtcag gatgaaccct tccagtgccc
     8041 ggtattccat ccggtttaac tggttaaagc tcgaccgggg ttgaatttca tagatccggc
     8101 cggcaaaata ggagttgtat ttcagaaaat cgacagtttc acatacactg tcgatttcag
     8161 cctggaagat attcttgctc tggccgagca tagtggcagc attcataagc gcacggtatt
     8221 ttgtcgcaat tagttcggcg gccttgagca ggatggaggc ccggatggtc catgccaggt
     8281 tcgaccaggt ctggtgtgct ttcagagcgg cttcaatggc cactttaatc tctttttctc
     8341 caaccatgtg gtatattccg atgacctttg aatggtcatg cggcatgcgt agctcagccg
     8401 tgtttcccgt cctgatctct tttcctccga taattaaagg gatttccacc ttttccaatg
     8461 attggcggtg aagttcctgt tccagggcaa tcctttcagg actgttgctc aaatacccgg
     8521 ataccggctc gttctgaggt gccgggaagt tgtatagtgc attattcata gcatctgttt
     8581 ttaataaaaa cgttatgcac atcattgcat aacggtcagc aaaaataaaa aaaaccggac
     8641 aattattaaa ctcatgcaat aaccccctcc ccccttacca ccctttgagg ttcctgatga
     8701 cgtgctgccg actaattttc ctatctttgc aaacttcaag aaaatcagct atggaagacc
     8761 gtcgtcggat catcatatac cttcctgtta ttctcgcttt tgtgcttatc ctgggcatct
     8821 ggctcggcag caagatgaac ttcagcaaga gcggtgaaac acagaatccc atatttaacc
     8881 gggctgtcca gcacgataag atcacggacc ttatcaatta tgtcgagcag gactatgtcg
     8941 attctgtcag ccgggaggat ctgaccgtga atgctattga gggagttctg gacaagctgg
     9001 atccccactc cacttatatc cctgctgaag attttggcga ggccgaagat cagcttgacg
     9061 acaatttcga agggattggc atccagttcc ggatagaaca agacaccatc atggtcatcc
     9121 aggtcattcc gggaggcccc tcggagaagg tcggcctgat ggcaggcgac aggattgtca
     9181 tgatcaacga ttcgctggtt gcgggtaccg ggctgaccag cagggacgcg gtcaaacagc
     9241 tcaaaggcaa gcgcggcacc gttgttaaag tgggcattct tcgccgcggc ctggccgaac
     9301 tggttgattt cagcatcacc cgcgatgtta ttcccaccta cagcattgat atttcctaca
     9361 tggtcaacga ttcggtcggt tacgtcaaac tcagccggtt cgccgtcagt acacacgaag
     9421 agatgatccg ggccatgagt ggtctcctcg aaaatggaat gaaacggctt atccttgatc
     9481 tccgcgataa taccggtggc tatctccagg cttcgatcca tgtgtcggat gagttcctgg
     9541 gcgataaaaa gctgatcgtt tataccgaag gaaacagtcg tccgaaccag tatgcatatg
     9601 cgacaaaaag gggtgtcttt gaagattatc cgctggttat ccttatcaat gaagcatcgg
     9661 cctcggccag cgaaatcgta gccggcgcca tccaggacaa tgaccgggga ctgatcgtcg
     9721 gacgcaggtc gtttggcaaa gggttggtac aggagcaact tactttcccc gacggatcag
     9781 ccgtaaggct taccgttgcc cgctatcata ccccgacggg acgcagtatc cagcggcctt
     9841 acaaggaagg tgaaaaggat gaatattacc atgaactcta cgaccggtat gccaatgggg
     9901 agatggaaaa tcccgacagc atttctttaa cagatacggt taaatactac actcctgccg
     9961 ggaaggtggt atatggcggt ggcggaatta tgcccgatgt ttatgtggct ttggcggctg
    10021 aagagaaaga ggtgctttac cgcaagctga tccaccagaa tacaatcttt caatatgctt
    10081 tcaattatac cgaccgggaa aggccgtcac tggcaaaata ttcgacaccg aaagagttca
    10141 ttgagagatt caaggtggat gacaagcttt tcagcgatct gatggattat gccaggaacc
    10201 aggatatcta ctatacgcct tctgagctca ttgattcaaa ggagaagata aagacactgc
    10261 tgaaagctta catcggccgc aacctgctgg atgatgaagc cttctatccc gtttaccatg
    10321 aggtcgataa agttttccag atcgccctcg atactctgat taagaataat tagagaatcc
    10381 gtatcatccg cggcatccgt gtgcaatcaa aaaatggatc attggaagtt taaattttaa
    10441 tacagattta ttcttaaatt tgatagataa tataaaacca aaccctatga actttgaatt
    10501 accctccctg ccttacgcac tggatgcgct ggaacctttt atttcgaaga acactttaga
    10561 attccattat ggtaagcatc accaggcg
//
LOCUS       DAOPNH010000092        10538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6787_length_10538_cov_7.446437, whole genome shotgun sequence.
ACCESSION   DAOPNH010000092 DAOPNH010000000
VERSION     DAOPNH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_10538_cov_7.446437"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKZ74_06350"
     CDS             <1..328
                     /locus_tag="PKZ74_06350"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92415.1"
                     /translation="RRRGDKETGRQGDGETRRQGDKVTGRGLEMWPNPCQEILNFKFS
                     ILNSGRGLYLSVYDVFGREIRKIDVPDTDHEIKLTVEDFVPGLYLVVLKDDNTIVGSA
                     KMVVSR"
     gene            757..1443
                     /locus_tag="PKZ74_06355"
     CDS             757..1443
                     /locus_tag="PKZ74_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPM92416.1"
                     /translation="MLRFSSNLFYLCITMEESTSKEKVLKNVREALISKTDPPFPRID
                     LDRPIFATVEESPDVNFAQELTAAGGLFIYCEKDEEVIEGLRFLFNDRHWDSIFAPEP
                     AIVELLRKSQIPCVFEPDKLTETRVAMTRCESLIARLGSVLVSTGQMAGRRVFVYPDV
                     HIVLAHASQLVGDIKDALANLRKKYDRQMPSMVTLITGPSRTADIEKTLVMGAHGPKE
                     LYVFFLEDRL"
     gene            1526..2347
                     /locus_tag="PKZ74_06360"
     CDS             1526..2347
                     /locus_tag="PKZ74_06360"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPM92417.1"
                     /translation="MKNLVTRTITGVFFVAAIIGSMVWHPAAFMAVAYILMIAGMLEY
                     FRIVSSGDVFPQRYNGLAAGSIVFLLPSLASLGWISPKFLVILPTLVMLFFAAELFRN
                     KPNSLQNLTFTLLPVGFIAIPMATLFLLMSPLVTEDQPHWHLLFGFFIISWSYDTFAY
                     LTGIWLGKHKLFEKISPKKTWEGTGGGAIFGLVAAMVMSVFFPVLTPAEWIAGGLIIM
                     VTGTLGDLSESLLKRNCHVKDSGTFFPGHGGVLDRFDSILFAAPAFFCYLILLNL"
     gene            2344..3009
                     /locus_tag="PKZ74_06365"
     CDS             2344..3009
                     /locus_tag="PKZ74_06365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPM92418.1"
                     /translation="MRLHRKGFLPLLVTLAILLALVWMINLAFPVQTCIHNILYGIGI
                     VFYFLCIRFFRLPSRTVDEKHENIISSADGHVVVIEEIDETVFFHDRRIQVSVFMSPL
                     NVHVNWYPISGEIVHTKYHRGSHIPAYRPKASLENERMSVVIRGEHGHEVMMTQIAGL
                     VARRVFTNAKKGSYVFQGEEAGIIRFGSRVDLILPVGTDIRVKIGQKVRACETVIGIF
                     PEK"
     gene            3040..3603
                     /gene="frr"
                     /locus_tag="PKZ74_06370"
     CDS             3040..3603
                     /gene="frr"
                     /locus_tag="PKZ74_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPM92419.1"
                     /translation="MTDESELLLQETEDQMNKSVQFLEKELHKLRTGKATPQMLEGIR
                     INYYGTPTPIEQTANITTPDARQIIIQPWDKSIIHDIDKAIMAANIGLNPKNEGEVLR
                     IIVPPLTEERRRDLVKKAKTEAENARISVRNIRRSSNDMAKDLEKEGVPEDEVKKLQE
                     EIQKLTTRFIDLVDKIVVQKEKDIMTV"
     assembly_gap    3635..3734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4012..5016)
                     /locus_tag="PKZ74_06375"
     CDS             complement(4012..5016)
                     /locus_tag="PKZ74_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPM92420.1"
                     /translation="MVSRFSDTFSLLSRITPRRMVNLLLLYFSYYAARTTGKVRHRGK
                     PFTVSIEPTTSCNLRCPECPSGLRQFTRPTGALSLELYRDIIDQLSPGLFYLILYFQG
                     EPFLNPLFFKMVEYARRKRIYTATSTNAHFLNDNFARKTVESGLDRIIISLDGLDQES
                     YEKYRTGGSVEKVFEGTRNLVRWKKELKSRTPYIILQFIVFSTNEHQVGELKKVAREL
                     GVDKLELKTAQVYNYEDGNDLIPSNPDYARYKKENGKFKIDNPLNNHCLRMWRGCVIT
                     WDGLVVPCCFDKDGSHRMGDLKINSFDEIWRGKAYDDFRRKLFTARSEIDICKNCTEK
                     "
     gene            complement(5027..5269)
                     /locus_tag="PKZ74_06380"
     CDS             complement(5027..5269)
                     /locus_tag="PKZ74_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92421.1"
                     /translation="MEKKNEYRDIERLKAFQLGRIRQQEQAIKLSFKEVRDNITGEAL
                     KEKLKENLFSGPGLAFKLGYITVTLLQERFSKTKKK"
     gene            complement(5244..5669)
                     /locus_tag="PKZ74_06385"
     CDS             complement(5244..5669)
                     /locus_tag="PKZ74_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPM92422.1"
                     /translation="MTNDLRLMTNDPKSPGLTELVLDLMEQLKSYVNMRIDYVKLQVA
                     DYLIRFFSSLTLFIILFWIVFFAVFFGSFAFAYWFGEKTGMWSVGFLIVAGFYILLAV
                     LVYVFRRVLIIRPFTRLILEQLELNKFIEKENGKEERVP"
     gene            complement(5669..5905)
                     /locus_tag="PKZ74_06390"
     CDS             complement(5669..5905)
                     /locus_tag="PKZ74_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPM92423.1"
                     /translation="MKKGVSNFLLGFLAGAAAGALAGILLAPDKGSETRKNLKKKVKD
                     LSDEYNLGLSDLMDDMEPEKAKAGKRQKGKVNSE"
     gene            complement(5909..7003)
                     /locus_tag="PKZ74_06395"
     CDS             complement(5909..7003)
                     /locus_tag="PKZ74_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPM92424.1"
                     /translation="MSQKYRVIVAATGVLLGLFMAWYFFNILVYIITAGVISLTGQPL
                     VEALSRIRIGRYSMPKSIAALITIVVLIGFLTGLVFLFVPAISRQAHIISMINVDEVV
                     KYFADVIDKMQRLLLSYDLIGTDQTLQSLLDQQIRSVIKVTDISNIIEKLVGATTQVF
                     IGIFSVLFLAFFFLRDRQLLRNGILLFTPDKFQEEVKNILHKTKTMLSRYFIGLVSEL
                     LIMMILISIGLSLFGIKNAVFIGFIGGLMNVIPYLGPIIGATIGAILGISADLSLGMY
                     DNALNTALIVVAVFSVANLIDNWVLQPVIYSRSVNAHPIEIFIVILMAGSLAGIPGMI
                     LAVPGYTVLRIVAKEFLSGFKLIDKLTENI"
     gene            complement(7046..7411)
                     /locus_tag="PKZ74_06400"
     CDS             complement(7046..7411)
                     /locus_tag="PKZ74_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPM92425.1"
                     /translation="MKGLTTIGMLILSNAFMTMAWYGHLKFNGAKAGGTGLFIIILTS
                     WGIAFFEYCFQVPANRIGYQGFGGPFTLVQLKVIQEVITLVVFMVFTLLIFKNETIKM
                     NHLIGFVFLVLAVYFIFKK"
     gene            complement(7439..9754)
                     /locus_tag="PKZ74_06405"
     CDS             complement(7439..9754)
                     /locus_tag="PKZ74_06405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM92426.1"
                     /translation="MKTRFTALLFVAFAGNLFSQQDLIGFVNPLIGTQRMGHTFPGAT
                     VPFGMVQLSPDTDTVSYEKDGKYNGEVYAYCSGYQYDDPTIVGFSHTHFSGTGHSDLG
                     DFLVMPSSGKARLNPGTASHPESGYRSAYAHANEMAQPAYYKVRLDDDGILAELTATE
                     RVGFHRYTFSKNDTANIILDLIHGIYNYEDKNVWTFIRVENDTLVTGYRQTSGWARTR
                     TVYFAMAFDNPVVEYGHRKYDNAVYRGFYRKFDETKNFPEMAGEKIRAYFRFLLHEGE
                     SLRVKFALSSTSTEGALKNLQTEIPHWDFDRIVNESQAKWNKELGKVAVEMMTPADMT
                     TFYTALYHTFLSPTIYMDVDGAYRGLDQNNHVADGFTNYTTFSLWDTYRALHPLFNLV
                     QPSRNSDMIRSMLAHYDQSVHPMLPVWSHHANENWCMIGYHAVSVIADAAVKNVGNFD
                     RLHALEACVKTAGYDLFDGIKYYETLGYVPEDKNSSSVSKTLEYAYDDWTIAQLARIT
                     GNAEVEKQFAERSLNFRKVYDKEIGFMRPRLASGEWKTPFDPLNTHGQGFIEGNAWNY
                     SLYVPHRIPDMITMMGGPERFTARLDSLFIMHLPDENFKDTEDITRDGIIGCYVHGNE
                     PGHHIPYLYNRAGKPWKTQEKVRMIMREMYGDTPDGLCGNDDCGQMSAWYVFSALGFY
                     PVCPGSDMYAIGSPLVVSAKITLENGRKVGISVENQSEENIFIRKIILNGDEYDKNYF
                     SHSNLVNGAEITFVMGARPEKSWGKISPEDE"
     gene            10223..>10538
                     /locus_tag="PKZ74_06410"
     CDS             10223..>10538
                     /locus_tag="PKZ74_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92427.1"
                     /translation="MKIFAPPYGILAILFLALSFLIVAPMKSQTIQIDSTFTTDAEIF
                     PFENLELISELLIEGDVELSSDTSLVRVILEDENGFRYMVLEAYPLICPEIDFTVQDH
                     CDE"
BASE COUNT         2583 a         2611 c         2656 g         2588 t
ORIGIN      
        1 aaggagacgg ggagacaagg agacggggag acaaggagac ggggagacaa ggagacaagg
       61 tgacaaggtg acggggagag gattggagat gtggccgaat ccctgccagg aaattttgaa
      121 ttttaaattc tcaattttaa attcggggag gggtttatat ttatctgttt acgatgtttt
      181 tgggagggaa atccggaaaa tagatgtccc ggataccgac catgaaatta agttgactgt
      241 cgaagatttt gtgccggggt tgtatctggt cgttcttaaa gatgataata ccatcgtcgg
      301 aagcgccaaa atggttgttt cgagatagac gatcttttca acaggcgaaa gcacgatcag
      361 acgaacagac gatattcgat tttatcaggt agtgctaagg ctgtattaaa attatatccc
      421 ctacggggaa atatttcttt ttgttattga tcctgctaca gtattttatc gcctacggcg
      481 aaaaccgtta acctacgggg aaatatttct ttttgctatt gatcctgcta cagtatttta
      541 tcgcctacgg cgaaaaccgt taacctatta accgacggat ttacccgtcg gaagaaaacc
      601 cctctttcac cgacagattt tttcaaacta tcccaaatcg ttaaatcgcc aaatcgccaa
      661 atcgttaatt cgccaaatcg ccaaatcgtt aattcgccaa atcgccaaat cgtccaatcg
      721 ccaaatcgtc caatcgccaa atcgtccaat cgtgaaatgc ttcgtttctc ttccaatctt
      781 ttttatcttt gcatcaccat ggaagagagc acgtcgaaag aaaaggtgtt gaaaaacgtc
      841 agggaagcgc tgatatccaa gaccgacccg ccgttcccga gaatcgacct cgaccggccg
      901 atcttcgcta ccgtcgagga atcgcccgac gtgaacttcg cccaggaact gacggcggct
      961 ggcggactgt tcatctactg cgaaaaggat gaagaggtga tcgaagggct gaggtttttg
     1021 tttaatgacc ggcactggga cagcatcttc gcaccggaac ccgccattgt ggagctgctc
     1081 cgcaaaagcc agatcccttg cgttttcgaa cccgataagc tgaccgaaac ccgggtggca
     1141 atgacccgct gcgaatcgct gatcgcacgg ctgggaagcg tgctggtgtc gaccgggcaa
     1201 atggccggac gccgcgtatt tgtctatccc gatgtgcata tcgtacttgc ccatgcttcg
     1261 caactggtag gtgatatcaa agacgcgctg gccaacctgc gtaaaaaata cgaccggcag
     1321 atgccgtcga tggtcaccct gatcaccggg cccagccgta cggcagatat cgagaaaacc
     1381 cttgttatgg gggcgcacgg cccgaaagaa ctgtacgtat tcttcctgga agacagattg
     1441 taatatattt accgacaacc atttaaacca gattataacc cttgcgcccg gatcatcatt
     1501 catatccctg taaattgccg gcttcgtgaa aaacctcgtc accaggacaa tcaccggagt
     1561 tttttttgtc gccgccatca ttggttcgat ggtctggcac ccggctgcat tcatggctgt
     1621 ggcttatatc ctgatgatag ccggcatgct ggagtatttc cggatcgtga gttcaggcga
     1681 tgtctttccg caacgataca acgggctggc cgccggcagc atcgtcttcc ttctcccctc
     1741 cctggcttcc ctcggatgga tatcgcccaa attcctcgtc atccttccta ccctggtcat
     1801 gctgttcttt gcagccgaat tattccggaa caaacccaac agcctccaaa atttaacctt
     1861 tacccttctg cctgtcggtt ttattgccat cccgatggct acgctgttcc tgctgatgag
     1921 cccgctggta acggaagacc agccccactg gcatctcctg ttcggattct tcatcatctc
     1981 ctggagttac gacacttttg cttatttgac cgggatctgg ctgggtaaac acaaattgtt
     2041 cgaaaagatt tcgccgaaaa aaacctggga agggacagga ggaggagcca tattcggact
     2101 ggttgccgcg atggtgatgt cggtattttt ccccgtgctg acgccggcgg aatggatagc
     2161 cggagggctg atcatcatgg tgacggggac actgggcgat ctttccgaat cgctgctcaa
     2221 acgcaactgc catgtcaaag actcaggcac ctttttcccg gggcacggcg gggtgctcga
     2281 ccggtttgac tcgatcctgt ttgccgctcc ggcatttttc tgttacctga tcctgctgaa
     2341 cctatgagac tgcaccgcaa aggatttctg cccctgctgg tgactctcgc catactcctg
     2401 gcgctggtct ggatgataaa ccttgctttt ccggtccaga cctgcattca taacatcctt
     2461 tacggcatcg ggatcgtctt ttacttcctg tgtataaggt tcttccggtt gcccagccgc
     2521 acggtggatg aaaagcacga aaacatcatc agcagcgccg acgggcacgt ggtggtcatc
     2581 gaggagatcg acgaaacggt cttttttcac gaccgccgga tccaggtatc ggtgtttatg
     2641 tcgccgctga acgttcacgt caactggtac ccgatcagcg gggagatcgt gcatacgaaa
     2701 taccaccgcg gttcgcatat cccggcctat cgacctaaag ccagcctgga gaacgagcgg
     2761 atgtcggtgg tgatccgcgg cgagcacggg cacgaggtga tgatgaccca aatcgcgggg
     2821 ctggtggccc ggcgcgtgtt caccaatgca aaaaagggca gttacgtttt ccagggcgaa
     2881 gaagcgggca ttatccgttt cggctcccgt gtcgacctga tcctgccggt cggtaccgat
     2941 atccgggtga aaatcgggca gaaagtcagg gcgtgtgaaa cggtcattgg cattttcccg
     3001 gagaaataat atttttgtaa aaaattaata tcgataatca tgacggatga atctgaatta
     3061 ctattgcagg aaaccgaaga ccagatgaac aaatcggtcc agttcctgga aaaagagctt
     3121 cacaaactgc ggaccggcaa agctacgccc cagatgctgg aagggataag gatcaattat
     3181 tacggaacac caacaccgat cgagcagacg gctaacatca ccacgcccga cgcccgccag
     3241 atcatcatcc agccatggga caagagcatc atccatgata tcgacaaagc gataatggcg
     3301 gcaaacatcg gcctgaatcc gaagaatgag ggcgaagtgc tgcgcatcat cgtcccgcca
     3361 ctgaccgaag aacgccggcg cgacctggtc aaaaaggcca aaaccgaagc cgagaacgcc
     3421 aggatcagcg tacgcaacat ccgccgctct tcgaacgaca tggcgaaaga ccttgagaaa
     3481 gaaggcgtgc cggaagacga ggtgaaaaaa ctgcaggaag agatccagaa actgacgacc
     3541 agattcatcg acctggtgga taagatcgtt gttcagaagg agaaggacat tatgactgtt
     3601 tgatatagaa ccagaaagtt cgtgctccga cgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnntgaagc cgtcggttcc tgatgggttt tccttccgac gactaaagcc
     3781 gtcggttcct gatgggtttt ccttccgacg actgaagccg tcggttcctg atgggttttc
     3841 cttccgacga ctaaagccgt cggttcccac tttcgtaaaa ccataagaac caacggtttt
     3901 attcgttgga ctaccaatat aaaaagaata tttgtacttt ttaaaaagcc ctactaccaa
     3961 taaaaagggg ctgctcagaa ttttaaggca tcttttactt caaaaaggaa ctcatttctc
     4021 agtacaattc ttacagatat caatctcact tcgggcggtg aataatttcc ttcgaaaatc
     4081 atcatacgcc tttcctctcc agatttcatc aaagctattg atcttcaggt ctcccatgcg
     4141 gtgactgccg tctttgtcga agcagcaggg cacaaccagc ccgtcccagg tgattacgca
     4201 gccgcgccac atgcgcaggc aatggttatt cagcggatta tcgatcttaa atttcccatt
     4261 ttctttttta taccgcgcat aatccggatt ggaagggatc aggtcgttgc catcttcata
     4321 attataaacc tgcgcggttt tcaactccag cttgtcgact ccaagttccc tggcgacttt
     4381 cttcagttcg ccgacctggt gctcgttggt gctgaaaaca atgaactgca ggatgatata
     4441 cggcgtgcgg gatttgagct cttttttcca gcgcaccagg ttgcgcgtcc cttcgaaaac
     4501 tttctccacg ctccctcctg tcctgtactt ttcatagctc tcctggtcta gcccgtcgag
     4561 cgagatgatg atccggtcga ggcccgattc gaccgttttc cgggcaaaat tgtcgttcag
     4621 aaagtgcgca ttggtggaag tggctgtgta aatccgcttc ctgcgggcat attctaccat
     4681 cttgaaaaac aacggattca gaaaaggttc cccctggaaa tagaggatca ggtaaaaaag
     4741 tccgggagag agctgatcga taatatcgcg gtacagctcc aggctcaggg cgcccgtcgg
     4801 acgtgtaaac tgccgcaggc ccgacgggca ttcggggcag cggagattgc aagaggtggt
     4861 cggttcaatg gaaacggtaa acggtttgcc gcgatgacgg actttcccgg tcgtccgggc
     4921 ggcataatag gaaaaataca acagaagcag gttgaccatc ctccgcggcg tgatccggga
     4981 aaggagcgag aatgtatctg aaaaacggga aaccaagacc ggacgactat ttcttcttcg
     5041 ttttggaaaa ccgttcctgc agcagcgtta cggtgatata gcccagcttg aatgccagtc
     5101 ccgggccgga aaacaggttc tccttcagct tctctttcag cgcttcgccg gtgatattgt
     5161 cgcgcacctc tttaaagctc agtttgatcg cctgttcctg ctgcctgatc cggccgagtt
     5221 gaaaagcttt caggcgttcg atatcacggt actcgttctt cttttccatt ttccttttca
     5281 atgaatttat tcaactcaag ctgttccaga ataagccggg taaaaggcct gataatcaat
     5341 acccttctaa aaacataaac cagtacggcc aggagaatat aaaaaccggc gacgataaga
     5401 aacccgaccg accacatgcc ggttttctcc ccgaaccaat aggcaaaggc aaacgaaccg
     5461 aagaaaacgg cgaaaaagac gatccagaag aggatgataa acaacgtcag gctcgaaaag
     5521 aagcggatca ggtaatcggc tacctgcagc ttgacgtaat cgatccgcat gttaacataa
     5581 gacttcagtt gctccatcag atcgagcacc agttcagtca gcccggggga ttttgggtcg
     5641 ttggtcatta ggcgtaggtc gttagtcact attcactatt cacttttccc ttttgccttt
     5701 tccctgcctt cgccttctcc ggctccatgt cgtccatcag atcgctcagg ccgaggttgt
     5761 attcgtcgga aaggtctttg accttcttct tcaggttctt acgggtttcg ctgcctttgt
     5821 cgggcgccag gaggatgccg gcaagagcgc cggcagcggc gccggccagg aaaccgagca
     5881 aaaagttaga tacccctttt ttcatcgctc aaatattttc ggttaattta tcaatcagtt
     5941 taaatccgct caggaactcc tttgccacga tcctcagaac ggtatagccc ggcacggcga
     6001 ggatcatccc gggaatgccg gcgaggcttc ccgccatcag gatcacaatg aatatttcga
     6061 tcggatgggc gttgacgctc cgcgagtaga tcaccggttg caatacccag ttatcgatca
     6121 ggttggcgac ggaaaagacg gccaccacga tcagggcggt attcagggca ttatcataca
     6181 ttcccaggct caaatctgcc gaaatgccaa ggatggcccc gatagtggcc ccgatgatcg
     6241 gccccaggta agggatcacg ttcatcaacc ctccgataaa accgatgaat acggcatttt
     6301 ttatcccgaa taaagataat cctatggaga tcaggatcat catgatcagc aactccgata
     6361 ccagcccgat aaaataccgc gacagcatgg tcttggtctt gtgtaaaata ttcttcacct
     6421 cctcctggaa tttatccggt gtgaagagca gtatcccgtt gcggagaagc tgccggtcgc
     6481 gcaggaaaaa gaatgcaagg aagagtacgg aaaagatccc gatgaacacc tgggttgtag
     6541 cgccgacgag cttttcgatg atgttgctga tgtcggtcac tttgattacc gaccggatct
     6601 gctggtcgag caggctctgc agggtctggt cggtgccgat gaggtcgtag gagagcaaca
     6661 gccgctgcat cttgtctatc acatcggcaa agtatttcac tacttcgtcc acgttgatca
     6721 tggagatgat gtgggcctgg cgggagatcg cggggacgaa gaggaagacc agcccggtca
     6781 ggaaaccgat gaggacgacg atcgtgatga gggcggcgat ggatttgggc atggaatagc
     6841 gcccgatcct gatcctcgac aaggcttcga ccagcggctg tccggtcagt gagatcaccc
     6901 cggcggtgat gatgtacacg aggatattaa agaaatacca ggccatgaaa aggccaagga
     6961 gaacgccggt tgcggcaacg atcacacggt atttctggct catcggatga attcctcggt
     7021 taacaaaaat aagaatataa tgacgttatt ttttaaaaat aaaatagacg gcgagcacga
     7081 gaaacacgaa tccgatcagg tggttcattt tgatcgtttc atttttaaag atcagcaggg
     7141 taaaaaccat aaaaacgaca agcgtgatca cctcctggat gacttttagc tgtaccagcg
     7201 tgaacgggcc gccgaatccc tggtagccga tccggttggc cggcacctgg aaacagtatt
     7261 caaagaaggc aatgccccag ctggtcagga tgatgatgaa caggcccgtg ccgcccgctt
     7321 tcgccccgtt gaacttcagg tggccgtacc aggccatggt cataaatgca ttcgacagga
     7381 taagcatccc gatggttgtc agacctttca tcgtaaaaat aatttgattc cgaattattt
     7441 attcatcttc cggtgagatc ttcccccatg acttttcagg acgagctccc atcacaaaag
     7501 taatttcagc cccgtttaca aggtttgaat gcgaaaaata atttttatca tattcatctc
     7561 cgttaagaat tatttttcga atgaatatgt tttcttcgga ttgattctca actgaaattc
     7621 cgaccttccg accattttcg agggtaattt tggcggatac taccagcggg ctgccgatgg
     7681 catacatatc gctgccgggg catacggggt aaaagccgag ggcgctgaag acataccagg
     7741 ccgacatctg gccgcagtcg tcgttgccgc aaagcccgtc aggcgtatcg ccatacatct
     7801 cccgcatgat catcctgact ttttcctgcg tcttccaggg tttcccggcc cggttgtaga
     7861 gatacgggat gtgatggccg ggctcgttgc catggacata gcagccgatg atcccgtcgc
     7921 gcgtgatgtc ttcggtatcc ttgaagttct catccggcaa gtgcatgatg aacagcgaat
     7981 cgaggcgggc ggtgaaacgt tccgggccgc ccatcatggt tatcatatcg ggtatccggt
     8041 ggggcacgta aaggctgtaa ttccaggcat ttccctcgat gaacccctgg ccgtgggtgt
     8101 tcagcgggtc gaacggcgtt ttccattcgc ctgatgccag cctggggcgc ataaaaccta
     8161 tctctttatc gtacaccttc ctgaaattca gcgaccgttc agcgaactgc ttttccacct
     8221 ccgcattgcc tgtaatcctt gccagttggg ctatggtcca gtcgtcgtaa gcgtattcca
     8281 gtgtttttga tacggaggat gagtttttat cttccggaac ataacccaat gtctcataat
     8341 attttatacc gtcaaagaga tcatacccgg ctgtttttac acaagcttcg agggcgtgca
     8401 aacggtcgaa attccccaca ttcttgaccg cggcatcggc gatgaccgaa acggcgtgat
     8461 aaccgatcat gcaccagttt tcgttggcgt ggtgcgacca caccgggagc atcgggtgga
     8521 cgctctggtc gtaatgggcc agcatggagc ggatcatatc gctgttgcgg gaaggctgca
     8581 ccaggttgaa cagcgggtgc agcgcgcggt aggtgtccca gagcgaaaag gtcgtgtaat
     8641 tcgtgaaccc gtcggcgacg tggttattct ggtcgagacc gcggtaggca ccatcgacgt
     8701 ccatgtagat tgtcgggcta aggaaggtat gatagagagc cgtgtagaag gtggtcatgt
     8761 cggccggggt catcatttcg acagcgacct tgccgagttc tttattccat ttcgcctggc
     8821 tttcattgac tatcctatca aaatcccagt gaggtatctc cgtctgcagg ttttttaatg
     8881 caccttcggt ggatgtggag gatagggcga atttcacccg taaactttcg ccttcgtgca
     8941 aaagaaatct gaaatacgcc ctgatcttct ccccggccat ctccgggaag ttcttcgttt
     9001 cgtcgaattt cctgtaaaac ccgcggtaaa cggcattgtc gtattttctg tgcccgtatt
     9061 cgacgaccgg attatcgaag gccatggcaa aatagactgt ccgggtcctg gcccatccgc
     9121 tggtttgccg gtaacccgtg acgagggtgt cgttttccac ccggataaag gtccagacat
     9181 ttttatcctc gtaattgtaa atgccgtgga tcagatcgag gataatgttg gcggtatcgt
     9241 tttttgaaaa ggtatagcga tggaaaccca cccgttccgt agccgtcagt tctgcaagaa
     9301 tgccgtcgtc gtcgagccgc accttgtagt aagccggctg cgccatttcg ttggcgtgcg
     9361 cataggccga ccggtagccg ctttcggggt ggctggccgt acccgggttg agccgggcct
     9421 taccggatga tggcatcacc aggaagtcgc ccaggtcgga atggccggtg ccgctgaagt
     9481 gggtatggct gaagccgacg atggtgggat cgtcgtactg gtagccactg caataggcat
     9541 agacctcgcc gttgtatttg ccatcctttt catagctgac cgtatcggta tcggggctga
     9601 gctggaccat gccgaacggc acggtggcgc cgggaaaggt gtggcccatg cgctgggtgc
     9661 cgataagcgg attgacgaag ccgatgaggt cttgctggga aaaaaggttc ccggcaaaag
     9721 ccacaaataa cagagctgta aaacgggttt tcattggaat aaatgataat tgtaagtaat
     9781 tgtttgttgt gtcgcggtac tgatctgaaa attgatggct tgtcgattct cagaaccgaa
     9841 tgttgtataa gcaaagattc atttgcaaag ataaagttcc atattcagcg gtgttatcct
     9901 gccgaaatta aatattttcg aggtgagttt ttctgaagag gatgtctcaa aaggatttaa
     9961 ccacaaagga caccaaggaa ttcacaaagg cgcacaaagg tatgctaact gatattcaac
    10021 cacttagtgt tccttcgtga tgtactttgt gcgccttcgc ggttaataaa aggccttttg
    10081 agacagcctc ttcgagactc catctcgtca tgttgagatg gagtctcaaa actttagtcc
    10141 tgagattgct ttttatatca atctttccta tatttactcc ttctttacct actaaaaatt
    10201 attgacctct aatccattga ttatgaaaat attcgccccc ccctatggaa tcttagcgat
    10261 tctctttctg gcgcttagtt tcctgatcgt tgcgccgatg aaatcgcaaa ccattcaaat
    10321 tgacagcaca tttaccacag atgctgaaat ttttcctttc gaaaacctcg aactgatcag
    10381 tgaactgctt atagaaggag atgttgagtt aagcagtgac acaagcctgg tcagggtgat
    10441 cctggaagat gagaacggat ttcggtacat ggtactggag gcatacccgt taatttgccc
    10501 tgaaatcgat tttaccgttc aggatcattg tgatgaaa
//
LOCUS       DAOPNH010000093        10516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6815_length_10516_cov_6.759583, whole genome shotgun sequence.
ACCESSION   DAOPNH010000093 DAOPNH010000000
VERSION     DAOPNH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6815_length_10516_cov_6.759583"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKZ74_06415"
     CDS             complement(<1..557)
                     /locus_tag="PKZ74_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPM92428.1"
                     /translation="MPTKIRLQRKGKKGFPFYHVVIADGRAPRDGKFIERIGIYNPVS
                     KPAEIDINFDRALYWLQAGAEPTDTVKSILKFKGVSYKFHLLKGVKKGAMTPELAEAK
                     FEAWQKEKQEKFVSKLKDAELKGKESKKKRLEAEMKVNEERAKELQAKRAKELKAKLA
                     VEETEEPVAEETAVEETVAEAAVEET"
     gene            822..5078
                     /gene="dnaE"
                     /locus_tag="PKZ74_06420"
     CDS             822..5078
                     /gene="dnaE"
                     /locus_tag="PKZ74_06420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPM92429.1"
                     /translation="MFLIFDTETTGLPRNYEAPVTDVDNWPRAVQLAWQLHDTDGSLV
                     EARDFIIKPDNFTIPYNAAKVHGITTEFAAENGDDLKLVLEQFIDALQQATVIVGHNV
                     DFDMKIIGAEFVRTRFENYFEGKIKVDTDRESLNYCALPGGIGGGFKLPKLGELHQKL
                     FGDGFAEAHNAAADVRATARCFLELIRLGIITAGKLGVDPEVVRNFIINHPEPVQPEQ
                     ITLRDLRQTVVSVQQPEETSLPKSPEPGTLQPAITPFCHLHVHSQYSILQATADVKDI
                     IAKAVEMKMPAMALTDHGNLYGAFAFIGEAVKHKIKPIIGCEYYISLERTRKKFTKDN
                     PDVRYHQVLLAKNLTGYQNLSRLSSIGFLEGFYDGVPRIDKPLIEKYREGLIATTGGL
                     KSEINDLILNIGETQAEEAFCYWLRLFGDDFYVQLNRHGLPEEEHVNKVLLQLARKYN
                     VRYFAANNAYYLAKDDAEIHDMLLCLQLNEYQATPVGHGRGYRFGFPNNEFYLKSREE
                     MEELFHDLPEAIATAGEIADKIETYPLKRNPLMPDYEIPSGFDDANAYLRHVTYQGAE
                     KRYGSITPEIAERIDFELETIKRMGYPGYFLIVQEILHQAREMGVSVGPGRGSAAGSV
                     VAYCLRITDVDPIRYNLLFERFLNPDRISLPDIDIDFDEDGREKVLKWVVEKYGAGRV
                     AQVITFGKMAPKGAIRDIARVKQLPLSESDRLAKLVNMRPGTTFTDAYKESPDLQRER
                     KEGEPLVQETLKGAERIEGQIRNTGTHACGIIIGKNELIDLIPLSITKESDMLATQFD
                     GSQIESVGMLKMDFLGLKTLSIIKDTLDNIRKSKGLSLDIEAIPLTDTKTFELYSKGD
                     TNGIFQFESEGMKKHLRDLKPNRFEDLIAMNALYRPGPMDYIPNFINRKHGREKIEYP
                     LPEMEEILKETYGITVYQEQVMLLSQRLAGFTKGRADVLRKGMGKKNKDLIDELKKDF
                     IEGIKSNDLDEEIAAKIWTDWEKFAGYAFNKSHSTCYAVVSYRMAWLKAHYPAEFMAA
                     VLSRHLSEIKKITFFIDECKHLHIPVLGPDVNESELHFVVNKKGGIRFGMAAIKGVGE
                     SAVNAIIEERNANGPFRSIFDFVKRINLRAVNKRALEALAKAGAFDGFQGIHRAQYFQ
                     QENPEDNIFLEKIIRHAAIYQEKQQASQHSLFGDDLTLDLQDPKIPDCPQWSKHHQLK
                     YEREVTGFFISGHPLDEYKAEIDSFCNVTFQDLNAGLSRYLNKAVSFAGMINTVEIRT
                     AKTGNQYANFELEDFTDSYRLSLFSDDFMKFKYLLVEDTYVLIFGKVEVNRKNNRPEI
                     RIKNMILLAEAMERFCKSVSVTFELDRIDSDLVRSVVKIVKSNPGECEIKIRIKDQDG
                     FYLDMFPKKFRVSPAGFIHAISEFEQVNYSVNGFHAVIGN"
     gene            5145..5462
                     /gene="trxA"
                     /locus_tag="PKZ74_06425"
     CDS             5145..5462
                     /gene="trxA"
                     /locus_tag="PKZ74_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM92430.1"
                     /translation="MALEITDANFQETVLNSQKPVIVDFWAEWCGPCRMVGPIVEEIG
                     KEYADTVVVGKVNVDHNPGITVKYGIRNIPTLLFFKGGQVVDKEVGAVPKQKILGKLQ
                     PHL"
     gene            5473..6408
                     /locus_tag="PKZ74_06430"
     CDS             5473..6408
                     /locus_tag="PKZ74_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPM92431.1"
                     /translation="MSWSIDHNRLQQFGSLEFLAKQVVEGFIVGLHKSPFHGFSVEFA
                     EHRLYNPGESIKNIDWKLYGRTDKLFTKRFEEETNLRCILVIDNSSSMYYPVVENPSL
                     DKPNKITFSVYAAAALMNLMKRQRDAVGLNIFSDKIEFGSGSKSNSVHFKYLYSQLEK
                     MLEPIPADAKKETSATSALHQIADNIHKRSLVVIFSDMFETATDPGDLFAALQHLRHN
                     KHEVVLFHVIDKKREIDFNFENRPYKFIDLESGRQVKVNPLEVKEEYTRLVSRFTNEM
                     RIKCGQYRIDFVEADINQGFEQILLPYLLKRERLY"
     gene            complement(6405..8504)
                     /locus_tag="PKZ74_06435"
     CDS             complement(6405..8504)
                     /locus_tag="PKZ74_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92432.1"
                     /translation="MKKLIMMIIAFSHTYLAILNASICRAQDSRKEAISLEDYIGDFF
                     EGEDEYMTGLGEWQEAVSRWLETPVCLNSDETEWLAEYRIIDPYQLSKLKEYRYIYGN
                     LLSVYELAFIEGWDEQSARKVFPLVTVLSGNNPSFIPGKFDLRSFRQSLSVKYRSNSG
                     KSRGYDKLYPGKSDSAVTHFRGSPAGIALRYDLQYRNRLELGIRMEKDPGEPMLQQTS
                     VLSQQIKVPDLFSGFLHIKDLGLLKNIIVGNFRANFGYGLNLSGGQSISTGRTNVPGM
                     AGKIRPQTSTGEAGFFRGAAVELASGRFSAVIFGSSRKIDGTTIIRDTLSGEVLSFSA
                     ISASGMHRTDSEMENRRTINETYAGGFGIFHSDWFKAGIFAGYNRFSAEMVYQQEPYR
                     QFDFRGKENIIAGLAMTAWLKPVNIFGEFSKSIGYGMAYLAGCRLSPIPGASILVSYR
                     HFTKDYNNFHGTGILSAGNNQNESGLRTFLRLELPGRWNIEALADISKSDWVSYTLNA
                     PGRKWSVGLLVENQWNRSALLALSFRYTGGSVRAEWPEANLARTTTSEQYRIRISARE
                     TTNSIFSFNTRLECSLRPEEPTGWLLMQDIEIKPWTGPLKFWLRCSVFDARGYDNRIY
                     AYENDVLYDFSTIAYYGKGVRGIFMIRSSLFQHIDLWVRFSTVYYSDKYIGSGWDETG
                     LNQQNEIEIQVRIRFPD"
     gene            complement(8501..9337)
                     /locus_tag="PKZ74_06440"
     CDS             complement(8501..9337)
                     /locus_tag="PKZ74_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92433.1"
                     /translation="MKIKMIGTLMAFLIIGGLGNLSWSRVFGQTGPAGGRCIAMGGVT
                     SVSEEIWAAANNPALLAGDCLPAISICLENKYLTGQMQYNSVAGILPAGRGGLGFTGK
                     LLLMSTYCDALMSLGYGRPFGEKVKIGINLVYHIQSAGEGFDPVHKVSYGLGTAVDLS
                     AKVSLSFSTFNPFRLYYKSSGYATLPSVFNLGMVYRYAPAFLVAIELEKEMDFPLCVK
                     TGMEYNFGETVFIRGGIRLLPFGYSFGTAWRKKRLLIEVSSSYHQYLGFSPVISFQYN
                     LK"
     gene            9423..10076
                     /locus_tag="PKZ74_06445"
     CDS             9423..10076
                     /locus_tag="PKZ74_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPM92434.1"
                     /translation="MEKIIFDFLNELKTNNDREWFQSNKSRYEKAKKSFESFIDELIP
                     VIRGIDPNIDLITAKDCVFRIYRDVRFSHDKSPYKTNMGAYITRGGKSSPMAGYYVHC
                     EPGGSFLAGGIYMPQPDVLKKIRDEVFYKYDDFRKIIEDKEFVKTFGQIDDDQKMKSP
                     PRGYPKEFPGIELLKFRNYAVAHFVSDELALSEKYPEYAKKVFKTLYPLNSFFNNVF"
BASE COUNT         2893 a         2617 c         2345 g         2661 t
ORIGIN      
        1 gtttcttcga cagcggcttc agcaacggtt tcttcaacag cagtttcttc agcaaccggt
       61 tcctccgttt cttcaacggc cagcttagcc ttcagctctt tagccctctt ggcctggagt
      121 tctttagccc tctcttcatt caccttcatt tcggcctcca gcctcttctt tttgctctcc
      181 ttgcctttca gttcggcatc tttaagcttg gaaacgaact tctcctgctt ttctttctgc
      241 caggcttcga atttagcctc agccagttcg ggtgtcatag cacctttttt aactcctttc
      301 agaagatgga atttgtatga aactccttta aacttcagaa tggattttac cgtatcggtc
      361 ggttctgccc cggcctgaag ccagtaaagg gcacggtcaa aattgatgtc gatctcggct
      421 ggtttgctca caggattgta aatgccaatt ctttcgatga atttcccgtc ccgtggtgca
      481 cgaccatccg caatgacaac gtggtaaaaa ggaaacccct ttttgccttt tctctgtaat
      541 ctgattttcg ttggcataat gtgttgttaa ataattagga tgaaagaatt ttaaattggg
      601 gtgcaaagat ataaaagtta ttccaataac caaatatcca ctcatccaaa ttcacaaaat
      661 cataagctca caaaatcaca aaatcacaat atcacaaaat cacaaaatca caatatcaca
      721 atatcacaaa attctttttt tatcaacatt cccccgcatc cctctcaaaa tacctatttt
      781 taaccgtttg tgatattcaa caataaattc ttgataatca aatgttcctg atattcgata
      841 ccgaaacaac gggcttaccc agaaattatg aagcaccggt gaccgatgtg gacaactggc
      901 cacgggccgt tcaactggcc tggcaattgc acgacaccga cggcagcctg gtcgaagccc
      961 gcgattttat catcaaaccc gataacttca ccatccctta caacgccgcg aaggttcacg
     1021 gcattaccac cgagtttgcc gctgaaaatg gcgacgacct gaaactcgtc ctggaacaat
     1081 ttatcgacgc gctgcaacag gctacggtca tagtcggcca taatgtcgat ttcgatatga
     1141 agatcatcgg cgccgaattc gtgcgaaccc gctttgagaa ctattttgag ggaaaaatca
     1201 aggtcgatac cgatcgcgaa tcgcttaatt actgtgcact tcccggtggg atcggaggcg
     1261 gtttcaaact gcccaaactg ggcgagttgc atcagaagct ttttggtgat ggatttgccg
     1321 aagcccataa tgccgccgcc gacgtccggg ctacagcccg atgtttcctg gagctcatac
     1381 gcctgggaat cattaccgcc ggaaaattag gcgtcgatcc ggaggtcgtc agaaatttca
     1441 tcatcaacca ccctgaaccg gttcaaccgg aacagatcac cctgcgcgat ttacggcaaa
     1501 ccgtggtcag cgttcaacaa ccagaagaaa catcacttcc gaaatcacct gaacccggga
     1561 ccctgcaacc cgccattact cccttctgcc atctccacgt ccattcgcag tattccatcc
     1621 tgcaggcgac ggccgatgtg aaagatatca tagcgaaagc cgttgagatg aaaatgcccg
     1681 ccatggccct gaccgaccat ggaaacctct acggggcttt tgcatttata ggcgaagcgg
     1741 ttaaacacaa gatcaaaccc attatcgggt gcgaatatta tatcagcctg gaacggaccc
     1801 gcaaaaaatt cacgaaagac aatcctgacg tgcgttatca ccaggttctg ctggcgaaga
     1861 accttacagg gtatcagaac ctgtcgaggc tatccagcat tggcttcctg gaagggttct
     1921 acgatggcgt gccgcgaatc gacaaaccgc tgatcgaaaa ataccgtgaa ggacttatcg
     1981 ctaccaccgg cgggctcaag tcggaaatca atgatttaat actgaatatc ggcgaaacgc
     2041 aggcggaaga agccttctgc tactggctaa ggcttttcgg cgatgacttc tatgtgcagc
     2101 tgaaccgcca cggcttgccc gaagaagaac atgtcaacaa agtattgctt cagcttgccc
     2161 ggaaatacaa tgtcagatat tttgcagcca acaatgctta ctacctggct aaagacgatg
     2221 cggaaatcca tgatatgctg ctctgccttc aactcaatga gtaccaggcc actccggtgg
     2281 gtcacggtcg agggtaccgc tttggttttc cgaacaatga gttttatctg aagagccggg
     2341 aagagatgga ggagttgttc catgaccttc ctgaagctat tgccactgcc ggtgaaattg
     2401 ccgataagat agagacctat ccgttgaaaa ggaatccgtt aatgcccgat tacgagattc
     2461 cctccggatt tgatgatgcc aacgcatacc tcaggcatgt cacctaccag ggagcggaaa
     2521 agcgctacgg ctccattaca cctgaaattg cagaaaggat tgattttgag ctggaaacaa
     2581 tcaaacgaat gggctatccg ggttactttc tgatcgttca ggagatcctg catcaggccc
     2641 gcgaaatggg cgtatcggta ggcccgggac gaggttcagc cgccggctcg gtcgtcgcct
     2701 actgcctgcg gataaccgac gtcgacccga tccgttataa cctgcttttc gaacgcttcc
     2761 tcaatcccga ccgtatctca ctccccgata tcgacatcga cttcgacgaa gacggcaggg
     2821 aaaaagtatt gaagtgggtt gttgaaaagt atggcgcagg acgtgtcgca caggtcatta
     2881 ccttcggtaa aatggcaccc aaaggagcga tccgtgatat cgcccgcgtt aagcaacttc
     2941 ccctgagcga atccgaccgc ctggccaaac tggtcaatat gcggcccgga acaacattca
     3001 ccgacgccta caaagaatcg cccgacctcc aacgggaacg caaagaaggg gaaccactgg
     3061 tgcaggaaac cctcaaaggc gcagaaagaa ttgagggcca gatacgcaat acgggtaccc
     3121 atgcctgtgg gatcattatc ggaaaaaatg aactgatcga cctcatcccc cttagcatca
     3181 ccaaagaatc ggatatgctg gccacccagt tcgacggtag ccagatcgaa agcgtcggga
     3241 tgctgaaaat ggattttctg ggattgaaaa ctctttcgat cattaaggat acccttgata
     3301 atatccggaa atcaaaaggc ctgtcactgg atattgaagc catcccgctg acggatacca
     3361 aaactttcga gctttattca aaaggcgata ccaacggcat attccagttc gagtcggaag
     3421 ggatgaaaaa gcacctgcgc gacctgaaac cgaaccgttt tgaggacctg atcgccatga
     3481 atgcgctcta tcggcccggc ccgatggatt atatcccgaa cttcatcaac cggaaacacg
     3541 gccgtgaaaa aatagaatac cctcttcccg aaatggaaga aattctaaaa gaaacatacg
     3601 gcatcaccgt ctaccaggaa caggtcatgc tcctttccca gcggctggct ggcttcacca
     3661 aaggccgcgc ggatgtcctg cggaaaggga tgggaaagaa aaataaggac ctgatcgatg
     3721 aactgaagaa ggattttatc gaggggatca aatccaatga cctggatgaa gagattgcgg
     3781 ctaagatctg gaccgactgg gaaaaatttg ctggatacgc cttcaataag tcacattcaa
     3841 cttgctatgc cgtcgtgtcg taccggatgg cctggctgaa agcccattac ccggccgagt
     3901 ttatggctgc tgtcctgagc cggcatctta gcgaaatcaa aaagatcacc ttctttatcg
     3961 atgaatgcaa gcaccttcat atcccggtgc tcggccccga tgtcaatgaa agcgaattgc
     4021 actttgtggt caataaaaaa ggcggtatcc ggttcggaat ggctgccatt aaaggcgtgg
     4081 gtgaatccgc cgtcaacgcc atcatcgagg aacgcaacgc aaacggcccg ttccgcagta
     4141 tcttcgattt tgtgaaaagg atcaacctca gggctgtaaa taagcgggca ctggaggcgc
     4201 tggctaaagc cggcgccttc gacggcttcc agggaatcca ccgtgcccag tatttccagc
     4261 aggaaaatcc cgaagataac atcttcctgg aaaagattat tcgtcatgcg gctatatacc
     4321 aggagaaaca gcaagcttcc cagcattccc tctttggcga tgacctcacc ctcgacctcc
     4381 aggatccgaa aattccggat tgtccgcagt ggtctaaaca tcatcaactt aaatatgagc
     4441 gggaggtcac agggttcttt atctccggtc atccgctcga tgaatacaaa gcggagatcg
     4501 attcattctg caacgtgact ttccaggatc tcaacgccgg attgagccgc tacctgaata
     4561 aagcggtctc gtttgccggt atgatcaata cagtagagat caggaccgcc aaaacgggca
     4621 accagtatgc taatttcgaa ctggaagatt ttaccgattc ttaccggctg tcgttattct
     4681 ccgatgattt tatgaagttc aaatacctgc tggtagaaga tacgtatgtc ctgatattcg
     4741 gaaaagtgga ggtgaaccgg aaaaataacc gccccgagat tcgcatcaaa aatatgatcc
     4801 tgctggctga agcaatggaa agattttgca aatcggtcag cgttaccttt gaactcgacc
     4861 ggatcgattc tgatctggta cgaagcgtgg tgaaaatcgt aaagtcaaat cccggggagt
     4921 gtgagataaa gatcaggatt aaggatcagg acggttttta cctggatatg ttccccaaaa
     4981 agttcagagt cagcccggcc ggattcatcc atgccatttc ggaatttgaa caggtgaatt
     5041 actccgtgaa tggtttccat gctgtgatag gaaattagcc ggtgagaaat cctccaacac
     5101 aatttttact atttttgaac gcttaatcat taaataattt acatatggca cttgaaatca
     5161 ctgatgctaa cttccaggaa actgtcctga attctcaaaa acctgtaatc gtcgactttt
     5221 gggccgaatg gtgcgggcct tgcagaatgg tcggaccgat tgttgaagaa atcggaaaag
     5281 aatatgccga taccgtcgta gtaggtaaag taaacgtcga tcacaacccc ggaatcactg
     5341 taaagtatgg catccggaat atcccgacat tgcttttttt caaaggcggc caggtagtgg
     5401 ataaggaagt cggtgctgtg cccaaacaga agattctggg caaacttcag ccccaccttt
     5461 aatattcact gattgtcctg gtcgatcgat cataaccgtc ttcaacagtt cggctcactg
     5521 gagttcctcg ccaaacaggt ggtggaaggt ttcatcgtag ggctgcacaa aagtccgttc
     5581 catggttttt ccgtggaatt tgccgaacac cggctttata accccggcga atcgattaag
     5641 aatatcgact ggaagttata tggccggact gataagctat tcaccaagcg ctttgaagaa
     5701 gagaccaatc tgagatgtat cctggttatc gacaattcaa gctcgatgta ttatccggtg
     5761 gtcgaaaatc ccagccttga caaacccaat aaaattacct tctcggttta tgccgccgca
     5821 gcgctgatga accttatgaa acgccagcgc gacgcggtag gtttgaatat tttttcagat
     5881 aagatcgagt tcgggtccgg ttcgaagtcc aattcggttc atttcaagta tttatattca
     5941 caactcgaaa aaatgcttga accgatacca gccgatgcga aaaaagaaac atcggccact
     6001 tcggccctcc atcaaatcgc cgataatatc cataaacgct cactggttgt catattttca
     6061 gatatgttcg aaacagcaac cgatcccggc gatttgtttg cagcgctcca gcacctcagg
     6121 cataataagc acgaggtagt cctcttccat gtcatcgata aaaaaagaga aatcgatttt
     6181 aactttgaaa accggccata taaatttatt gacctcgaaa gcggacgcca ggtgaaagtg
     6241 aacccgctgg aggtcaaaga ggaatataca cgcttggttt cccgttttac taacgagatg
     6301 agaatcaaat gcggacagta ccggatcgat tttgtggaag ccgatataaa ccagggcttc
     6361 gaacaaattc tgttgcctta tcttcttaaa agggaaaggc tttattaatc gggaaacctt
     6421 atcctgacct gaatttcaat ctcattctgc tggtttaacc ccgtttcatc ccaaccgctt
     6481 ccaatgtatt tatccgaata atatacagta gaaaacctga cccacaaatc aatatgttgg
     6541 aaaagcgaag acctgatcat gaaaatcccc ctgacccctt tgccatagta ggctatggtc
     6601 gagaaatcat acaagacatc attttcataa gcataaatcc ggttatcata cccgcgggca
     6661 tcaaatacac tgcaccttaa ccagaacttt aacggcccgg tccagggctt gatctctatg
     6721 tcctgcataa gcagccagcc ggttggctct tccggacgca gactgcattc cagcctggtg
     6781 ttgaaactga agatactatt tgtcgtttcc cttgccgata ttcttatccg gtattgttct
     6841 gatgtcgtgg tccgggccag gtttgcttcc ggccactctg ccctgaccga gcctccggta
     6901 tagcggaatg ataacgccag cagggccgaa cggttccatt ggttttcaac cagcaatcca
     6961 acactccact ttcgtccggg agcatttagc gtgtaactta cccagtctga ttttgaaata
     7021 tccgccaggg cttcaatatt ccatcttccg ggcagttcta accgcaaaaa agtcctcagg
     7081 ccggattcat tctgattatt gccggctgat aaaatgcctg tgccgtgaaa attgttgtaa
     7141 tccttggtaa aatgacggta actgaccagg atcgaggccc cgggtatagg agatagacgg
     7201 caaccggcca ggtaagccat cccgtatcca atgcttttgg aaaattctcc gaaaatgttt
     7261 acaggcttaa gccaggcagt catagccagc ccggcaataa tattctcttt tccgcggaaa
     7321 tcaaactgcc tgtaaggttc ctgctgatag accatttcgg cgctgaagcg gttgtaccct
     7381 gcaaaaatgc ccgctttgaa ccaatcgctg tgaaatatcc cgaacccgcc ggcatatgtc
     7441 tcattgatcg tacggcggtt ttccatttcg ctatcggtgc ggtgcatgcc tgacgctgaa
     7501 atggccgaaa atgaaaggac ctcccccgaa agagtgtccc ggataatagt ggttccatca
     7561 attttccggc tcgatccgaa gataacagcg gaaaatcgac cggaagccag ctcaacggct
     7621 gcccccctga aaaaacctgc ctcacccgtc gaggtctgtg gccgtatttt gcccgccatg
     7681 cccggaacat ttgtcctgcc cgtcgaaatg gactgcccgc ccgataggtt caggccatac
     7741 ccgaaattag ccctgaagtt gccgactatt atatttttaa gtaagcccag atcctttata
     7801 tggagaaaac cggaaaaaag gtctggtact ttgatttgtt gtgataaaac agatgtctgc
     7861 tgaagcatgg gctcgccagg atccttttcc atacggatgc ccagctccag tctgtttcgg
     7921 tattgcagat cataacgcag cgcaattccc gcgggagatc cccggaaatg cgttaccgcg
     7981 gaatcgctct ttccaggata caacttatca taccccctgg atttgccgga attcgatctg
     8041 tatttaacgg atagactttg ccggaatgat ctcagatcga attttccggg aataaaggag
     8101 ggattattgc ctgataatac ggtaaccagc gggaacactt tgcgcgctga ctgctcatcc
     8161 catccttcta taaaagccag ctcatatact gaaagcaggt tgccgtagat gtaacggtat
     8221 tctttcagct tactgagctg gtagggatca atgatccggt attctgccag ccattccgtt
     8281 tcatcactat tcaggcagac cggggtttca agccacctcg aaacagcctc ctgccactcc
     8341 ccgagaccgg tcatatattc atcctcccct tcaaagaagt cacctatata gtcctccagg
     8401 gatatggcct ctttccggct atcctgtgcc cggcatatcg aagcgttaag tatggcaaga
     8461 tatgtatggc taaatgcaat tatcatcatg ataagcttct tcatttcaga ttgtattgaa
     8521 aagatataac aggtgaaaat cccagatact gatgataaga tgaggaaact tcgataagta
     8581 atctcttttt cctccatgct gtaccgaatg aatagccgaa tggcagcaag cggatgccgc
     8641 cccgtataaa tacggtttcc ccgaaattgt attccattcc cgttttgacg cataagggga
     8701 aatccatctc cttctccagc tcgattgcaa ccaggaaagc cggagcatag cggtaaacca
     8761 tccccagatt gaaaaccgag ggtaatgttg cataccctga acttttataa tagagccgaa
     8821 atggattgaa ggttgagaaa gagagagata ccttagccga caggtccact gctgtaccca
     8881 aaccatagct gactttgtgc accggatcaa atccttcgcc tgccgactga atatgataga
     8941 ccaggttgat cccgatcttt accttttccc cgaatggtct gccgtaacca aggctcatta
     9001 atgcatcgca gtaggttgac atgagcaata gtttcccggt aaagcccagt cctccacggc
     9061 ctgccggcag gattccggcc accgaattat actgcatctg ccccgttaag tatttatttt
     9121 ccaggcatat cgagatggct ggaaggcaat cccctgccag cagtgccgga ttattggctg
     9181 cagcccaaat ctcctcgctg actgatgtga ccccacccat cgcaatacat cttccccctg
     9241 ccggccccgt ctgtccgaat acccggctcc atgacaaatt acccagacct ccgataatca
     9301 aaaaggccat caacgtcccg atcattttta ttttcataac acctcctata ttgcttaatc
     9361 cgttttttgc cccaattata cccggtcgat gcaagaaaaa aataactttg ttaaaaaaaa
     9421 gcatggaaaa gattattttt gacttcctta acgaattgaa aaccaataat gaccgtgaat
     9481 ggttccagtc gaataaatcc cgctatgaaa aagccaaaaa atcgttcgaa tcattcattg
     9541 atgaacttat tccggttatc aggggtattg atccgaatat cgatctcata acagcgaagg
     9601 attgtgtatt ccggatttat cgcgatgtga ggttttcaca cgacaaatcg ccctataaaa
     9661 ccaatatggg tgcctatatt accaggggag gaaagagttc accgatggcc gggtattatg
     9721 ttcattgcga gcccggcggt tctttcctgg cgggaggtat ctacatgccg caacctgatg
     9781 tgctgaagaa gatcagggat gaggtctttt ataaatatga tgatttcagg aaaattattg
     9841 aagacaagga atttgtaaag actttcggac aaattgatga tgaccagaag atgaaaagtc
     9901 cgccccgggg atatccaaaa gaatttccgg gaatagaact gctgaaattc cggaattacg
     9961 ctgtggcgca ttttgtttca gacgaactgg ccttaagtga aaagtacccg gaatatgcca
    10021 aaaaggtttt taaaacactt tacccgctta actcattctt taacaatgtt ttttaagagg
    10081 ttttttttaa tgatgaccag gtaagacatt gccgttaact tgagaatgat tccgaaaact
    10141 gattcaaatc aaccgttaac ttattttatt tgcatcacgt gtagaaaaac ttgtactttt
    10201 atctcggatt aattggttaa ttatttccta cttcggaggc ttttgaagat ttggttaatt
    10261 ggtttacttt acttcaaaag tcctttctca cctcaaacta gtaagacttg gcaaaagcaa
    10321 ccccgacgag ctcggggttg ctcttttatg gcaacctgta atttcaggat ggttataatt
    10381 tctggaaaca gtcaagatta tcacatgaga gaagccatct ctttctgcat cttcagcgct
    10441 tcgtctgccg ccctttcggc aaagtcctct ttactggatg cgaaaattat gcttcgcgaa
    10501 gcattgacca gcaggc
//
LOCUS       DAOPNH010000094        10482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6839_length_10482_cov_7.866884, whole genome shotgun sequence.
ACCESSION   DAOPNH010000094 DAOPNH010000000
VERSION     DAOPNH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_10482_cov_7.866884"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..883)
                     /locus_tag="PKZ74_06450"
     CDS             complement(212..883)
                     /locus_tag="PKZ74_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92435.1"
                     /translation="MKRFTIVFIAVVSILLANAQTKLDYSISVIYNRSSQTNYNPQLP
                     DDGTFQWNALNTFGIGVNIGKPLSDRVYWTNRLLCQQKGYSQEAQTADFIDGIPGPLS
                     YHKLQDRFGYLTIESNIGYHIIKFKSLKLTPLIGFNINYLLSKSIESEGVYPINSCYP
                     IDEYNGNWKKINVGYNVGISCAIQKKVNLGFEFQRSITPLLKTNDLTVKDWTWSVRLD
                     FLLSD"
     gene            complement(913..2352)
                     /locus_tag="PKZ74_06455"
     CDS             complement(913..2352)
                     /locus_tag="PKZ74_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HPM92436.1"
                     /translation="MKKLALSSVIILSTLSITFSQVIPTDSLYFGQTPPGCIPQLFAP
                     GIVSIPDRNEATITFSPDGASVFFHIEKYPEPGNPYILSASYLEDHWTTPDTIPFSNG
                     RATGEPFFAYNGNRVYLFATNAVNHEGIADLSFSQKEGDSWSDPVSLGNPPNSESYQY
                     HPCIVGDSSVYFSSQAGKICRSQYCDGIYENRIVLPKPVNFIGSQTWGDPYVSADESY
                     MIMKAIREEGYGQNDLYIAYRKPDGNWTNPKNLGNVINTPGDETSGDITPDGLYMTYG
                     SNDDLYWVSAGFTDSLRYTNFIPYMKYPIPDQTAYIGEQFIYSIPDSTFSDDDSSSVF
                     TFTAVLTNGNPLPEWLTFDTVSGVFTGVPEMEQILNIKLTANDNAGASAWTPFKLSVQ
                     LPTGYDPVNFHALTIFPNPNRGLITITLGPFTGKTATVVLNDLQGRTFVRQVFKNETI
                     IDLTDCSSGVYILKVYVENELISRKIYKM"
     gene            complement(2393..3025)
                     /locus_tag="PKZ74_06460"
     CDS             complement(2393..3025)
                     /locus_tag="PKZ74_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92437.1"
                     /translation="MKKITLTSLLTIMCISLALSQNQRKSATENEADHKFSFGTTYLT
                     LANFGQEKTNTHHYEFHGSYQLTPKDRIGIKVATWKLFAPMGIQLWDPGFRDESEFFP
                     GRLSECGIGVTYQRMLWKGLFAAVEVLPLFKIYLDEEKNKIDEGFKLYTTWHLGYHIA
                     LFKNRFYLEPQVHCNYWPVDTKAPQGFEEKESKWKNYFLFEPNLYFGINF"
     gene            complement(3101..3727)
                     /locus_tag="PKZ74_06465"
     CDS             complement(3101..3727)
                     /locus_tag="PKZ74_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92438.1"
                     /translation="MKKYILAILTMAMFTSMAFSQNLIQKDSASALAKSKDLIRRHSI
                     GSSLFMFGNLGPGDPVYYFQLNYGYRLTAKDNLIFEAITWTYYEPLGTYGSSEEMYPG
                     KVRALGAGFGYQRFHWKNLFTTVEATPFIQQFYDSEDQKIQKGFQLYLQAILGYRFEF
                     FEKRVFIEPAYALKYWPVNTNMPESFKAVEKGAPKYIFEPSLNFGIRF"
     gene            complement(3747..3968)
                     /locus_tag="PKZ74_06470"
     CDS             complement(3747..3968)
                     /locus_tag="PKZ74_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92439.1"
                     /translation="MKGKTVIKIKGHLDKKWKESFEGTEIHHEGSETVLSIDLKDDAH
                     LHGILNLVRDLNLKLISVNPDDDHNEHSI"
     gene            complement(4105..5076)
                     /locus_tag="PKZ74_06475"
     CDS             complement(4105..5076)
                     /locus_tag="PKZ74_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPM92440.1"
                     /translation="MTMLAAVSDQYGSPDILRIETVKKPVPKNHEVLIKVFATTVNRT
                     DCAVLSGKPLIFRLFTGVIHPKRSVQGTDFAGEIVSAGKNVTKFREGDRVFGFDDVGL
                     SSHAQYLTLSENNAIATIPDHITYEHAAAGIEGAHYALNFLNKLDIKKGQKVLVNGAT
                     GAIGSAAVQLLNYYGAKVTAVCRKKDEDLVKSIGAVKVIDYINEDFTRPEEKYKFVFD
                     AVGKSSFSRCKPILERGGIYISSEPGRMAQNIFLPMITAVFDTKKVVFPFPLDRAGSV
                     LFMQKLLREGKFLPVIDRRYSLEEIADAYRYVGKGQKTGNVIISVQH"
     gene            complement(5115..7799)
                     /locus_tag="PKZ74_06480"
     CDS             complement(5115..7799)
                     /locus_tag="PKZ74_06480"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPM92441.1"
                     /translation="MLLTKLHIPSPGDQTVHREDLFDKLNCGLNRKLILISAPAGFGK
                     TTLVCDWIHQQKIPAAWFSIDKGDNDPEEFLSYIISGIQGVRKEFGQSALRLLQSPNK
                     PSPEAIATLLINDILRIKHDFILVLDDFHHVSSREVVELINFILQYIPGNIHLVILTR
                     SDPALPLARIRSQHQMVELRLADLSFTAHDISALFNKKLKLGLSNEDVYLLESRTEGW
                     IAGLQLAALSVQQRRDVSEFIRDLQGENQYIMDYLMEEVLKTQPDDIREFLLKTSLLD
                     QMNASLCDWVLNRNDSRFIIEHLERNNMFVIPLDSERNWYRYHHLFSDLLKQRFQQQM
                     ITNAADIRTKACDWFEQHQMTDLAIENALAGQNYEKSLQLISKVVEEMWCNGKHAAIL
                     RYGAILPDEWIQRNADFCFYYAWVLITSGQIGHAEPYLACADWMVNEKLKSRDIALKE
                     AQGYKKLSGKIAVAYTYLKSHDTHSEKIFAYSKRAMEYLPEDDPQWFSWAWFSEGIAW
                     YSKGDLERSNAAFLKAFDFAKKSGNIYLISTTAIRMAENEQQLGHYRSAYKKCMELLT
                     LLKARGYSQITKADWTFASLYYILGTSYFMWAENDKAFDNIKIAYDLCKSGKDSLLKA
                     MILMVYSVILRDRGEAEAERRIAELDDIMNIEKLPPFMNAYYMGWKIFMFLDKNQMDK
                     ARDAASGFGLDLDQEKTFANEAAYSSYLRLLLAQNQLDEAELLLSALCPLAIAGNRAE
                     RIIDLKTAHAILYKKRGEYEKAVAVLAEAMALAAEENLLSFFVFNNDPIDALIKKIFN
                     AGTSASPHIPKKFISNLKLALERKDNRKKGLAESALSARELEMMKLMAEDLMNQEMAD
                     RLFVSLNTVKTHLKNIFIKLGVDSRSRAVAKAKEMGLI"
     gene            complement(7835..8518)
                     /locus_tag="PKZ74_06485"
     CDS             complement(7835..8518)
                     /locus_tag="PKZ74_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM92442.1"
                     /translation="MAVNCIVIDDTPLAVEKLEGFIRQVPLLNLLASFNNALDAISFI
                     KGNPVDLVFLDIQMEQFSGLQFLESLRSPPQIIIVSAYDQYAVSGFDYSVTDYLLKPY
                     SFERFLKAIDKIKVDPAHKPSKDYMFVKTEYRMERINFADILYIEGQGAYLRIVTPKN
                     RIMTLQNFQTMESQLPADNFIRVHKSFIVAINKIESIERAIIKIGAQRIPIGAGYREK
                     FKAALNLKD"
     gene            complement(8508..9422)
                     /locus_tag="PKZ74_06490"
     CDS             complement(8508..9422)
                     /locus_tag="PKZ74_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM92443.1"
                     /translation="MGAYFLSGSNLFGIIFNFYLYILLPVGIFYACYFLLKPHNLKGQ
                     IFKLLTIIILPFIITTIFKYLTIRVGFEFPYFLVMTGLFAIPFATFGLLFRKMEVWIT
                     HERRTQENLKSELALLKNQISPHFLFNTLNNIDSLIKTNADQASETLIKLSGIMRYMI
                     YDTNAGRVPLSLEIKHIEDYIDLQKIQFSNRELVSFNVSGDTDRIQIAPMLFIPFVEN
                     AFKHCTDKNTRNAIRFSFRIEGNTVIFESSNIFNEEQKISRDHAGGIGLKNIKRRLEL
                     LYPDSHFLNFIQENSTFKVNLTVKINGR"
     gene            complement(9734..>10482)
                     /locus_tag="PKZ74_06495"
     CDS             complement(9734..>10482)
                     /locus_tag="PKZ74_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM92444.1"
                     /translation="SSLNSGSNGNCYYVGNDHDAVLIDAGISCRETEQRLKRQGLSLT
                     KVRAIFISHEHSDHTRGVEVLSRKHRIPVCITPATHRNSLIRLEPQLLQPFSAGEELH
                     FGSLTVKAFQKHHDAADPHSFTVTGSGITVGIFTDIGSVCEQLIHHFSRCHAAFLEAN
                     YDETMLEEGRYPYFLKRRIRGGNGHLSNLQALELFNSYRAPFMQLLVLSHLSRQNNHP
                     QIVTDLFAPHANGTRVTIASRFGESEVFAV"
BASE COUNT         2856 a         2300 c         2331 g         2995 t
ORIGIN      
        1 agcatatctt ccagcatttc aatatagatg atcagttcct ccttcgacag tttatcctcc
       61 ctgatcttct ccttcagttc atggattttt gatattaagt atactatgat ctgctccttg
      121 tggtccattt accggttgtg gttagatata gtcggctaat tcagacgctt aaaaatttgc
      181 ttttaaagat acatttttaa ccaaaacatt cttaatcact aagtaaaaaa tccagtctga
      241 ccgaccacgt ccagtccttt actgttaagt cattagtctt taataaagga gttatgcttc
      301 gctgaaattc aaaccccaga ttgacttttt tctgaatagc gcatgatatt ccgacattgt
      361 accctacatt tatttttttc cagttcccat tgtattcatc aataggataa caggaattga
      421 tggggtaaac cccttcggat tctatcgatt tactcaacaa ataattgata ttaaagccga
      481 tcagaggagt taatttcagg gatttaaatt taatgatatg ataaccgata ttcgattcaa
      541 tagtcagata cccgaatctg tcctgaagtt tgtgataact taatgggccg ggaatcccat
      601 caatgaaatc tgcagtttga gcttcctggg aatacccttt ttgctggcag agaagacgat
      661 tcgtccagta gaccctgtcg ctgagcggtt tgccaatatt aacaccgatt ccgaacgtat
      721 ttaaggcgtt ccattgaaaa gtgccatcat ccggaagttg gggattataa tttgtttggc
      781 ttgatctgtt gtaaattacg gatatgctat aatcaagctt tgtttgcgca tttgcaagta
      841 gtatactgac tactgctata aaaactattg tgaatcgctt catatgctta aatttaaatt
      901 ctttgagaat cactacattt tataaatctt cctggaaatt aattcatttt caacatacac
      961 tttcaggata taaacaccgc tagaacaatc cgtcaaatca ataatggttt catttttaaa
     1021 cacttgtctg acaaaggtcc tgccttgaag atcattaaga acgacggtcg cagtcttgcc
     1081 ggtaaatgga cctaatgtaa ttgtgataag cccacggttc ggatttggaa agatcgtgag
     1141 ggcatgaaaa tttaccggat cgtatcctgt tggcaattgc acactaagtt taaatggggt
     1201 ccatgccgat gcaccggcgt tgtcgttggc tgtcagtttt atattcagaa tttgttccat
     1261 ctccggcacg cctgtaaaaa ctcccgaaac cgtgtcaaat gtcaaccact ccggcagggg
     1321 atttccattc gtgagtacgg cggtaaaggt gaaaacactg ctggaatcat catcagaaaa
     1381 agtactgtcg ggaatggagt aaatgaattg ttctccgata taagcggtct gatcaggtat
     1441 cggatatttc atatacggaa taaaattggt ataccgaagg ctgtcggtaa agccggccga
     1501 aacccaatac agatcgtcat ttgagccata ggtcatgtac aaaccatcgg gcgtaatatc
     1561 ccccgaagtt tcatcgcccg gagtattgat gacattcccg agatttttgg gattggtcca
     1621 gttgccatcc ggctttctgt aagcaatata caaatcgttc tgcccgtatc cttcttcccg
     1681 tatcgctttc atgatcatat agctttcatc ggctgataca tacggatcac cccaggtttg
     1741 tgaaccgatg aaattaacag gctttggtaa aacgatcctg ttttcataaa taccatcgca
     1801 atactggctc cggcatattt tcccggcctg gctcgaaaaa tagaccgacg aatccccgac
     1861 aatacaagga tggtactggt aagattcaga atttggagga ttgccaagac tgaccgggtc
     1921 cgaccatgaa tccccttctt tctgtgaaaa gcttagatca gcaattcctt catggttaac
     1981 agcattggtg gcaaacaggt aaacccggtt gccattgtaa gcaaagaatg gttcgccggt
     2041 ggctctgcca ttggagaaag ggatggtatc gggcgttgtc cagtggtctt ccagatagct
     2101 agccgacagg atatacggat ttccgggttc aggatatttc tcaatatgaa aaaaaacaga
     2161 ggctccatcc ggtgagaaag tgatggtggc ttcatttcga tcaggaatcg aaacgatgcc
     2221 cggagcaaat aattggggga tgcaaccggg aggagtttgg ccgaaataga gactgtcagt
     2281 aggaataacc tgtgaaaacg tgatactcag ggttgaaagt atgatgacgg aggaaagcgc
     2341 taattttttc atggtattta caatttgatt tttattacgg atatcctgat tgttaaaaat
     2401 taattccgaa atagagattg ggttcgaaca gaaaataatt cttccattta ctttccttct
     2461 cttcaaaacc ttgcggcgct ttggtatcaa ccggccagta attgcaatgg acctgcggct
     2521 ccaggtaaaa cctgttttta aagagcgcta tgtgataacc caggtgccag gtcgtataaa
     2581 gcttaaatcc ttcatcaatc ttatttttct cttcgtccag gtatattttg aaaagtggca
     2641 gaacctcaac tgcagcgaat aatcctttcc acagcatccg ctggtaggta accccaatgc
     2701 cgcattcgct gagcctgccc ggaaagaact cgctttcgtc acggaatccg ggatcccata
     2761 actgtatgcc catcggggca aaaagcttcc atgtggctac tttaatgccg atcctatctt
     2821 ttggcgtgag ttgatagctg ccgtgaaact cgtaatgatg cgtgttggtt ttttcctggc
     2881 cgaaattggc cagggtcagg taggttgttc cgaaggaaaa tttgtggtca gcttcatttt
     2941 ctgtggctga tttcctttga ttctgactca gtgcaagcga tatgcacatg atcgtcaata
     3001 gcgatgttaa agtgattttt ttcattattc agatctttat gattttcaga aaacttacaa
     3061 ttatcaaaat gaatggaaat aaattagagc ttgaacctca ttaaaacctg atcccaaaat
     3121 tcaggctggg ctcaaaaatg tatttaggtg cgcccttttc aacagcttta aatgactccg
     3181 gcatattggt gtttaccggc cagtatttga gggcatatgc gggttcaata aaaacacgct
     3241 tttcaaagaa ttcgaaacga taacccagga tcgcctgaag atataactgg aaacccttct
     3301 ggatcttttg gtcttctgaa tcatagaatt gctgaataaa aggggtggct tctacagtgg
     3361 taaacaggtt tttccagtga aagcgctggt aaccgaaccc tgctcccaaa gccctgactt
     3421 ttcccgggta catttcctca gaagaaccat aagttcccag gggttcgtag taggtccacg
     3481 tgatggcctc aaagatcagg ttatccttag cagtaagccg gtatccgtag ttcaactgga
     3541 agtaatagac gggatcgccc ggccctaggt tgccgaacat gaaaagcgag ctgcctatgg
     3601 aatgccgccg gatcaggtct ttgctttttg ccagggccga cgcagaatct ttttgaatca
     3661 ggttctgact aaatgccatg cttgtaaaca tggccatggt taaaattgct aaaatgtact
     3721 ttttcatatt gttataatta aggttttcaa atggaatgtt cgttatggtc atcatcggga
     3781 ttgaccgaaa taagtttcag gtttaaatcc ctgacaaggt tcaggatgcc atgcaaatgg
     3841 gcatcatctt tcagatcgat cgaaagaacg gtctcagatc cttcatgatg gatctccgta
     3901 ccttcaaaac tctccttcca ttttttgtcg agatgaccct tgatttttat aaccgttttg
     3961 cccttcattt tttcaggatt aaatacggat acaaaagtgt tgcggcaggg acaaaaataa
     4021 atcacccgcg agggtgaatt cagcgggtga tttgttaaac ggaatgatac aagtgcgggt
     4081 gaatcaagcc gggagactgg tttctcaatg ttgaacagaa atgatcacat tcccggtttt
     4141 ctgccctttc ccgacgtacc ggtaagcatc ggcgatttct tcgagggaat atcttcggtc
     4201 gattaccggt aaaaatttcc cttcccttaa gagtttctgc ataaacagaa cacttccggc
     4261 gcggtcgagg ggaaaaggaa atactacctt tttggtatca aatacagcgg taatcatcgg
     4321 caggaatata ttttgcgcca tcctgccggg ttccgatgaa atgtagattc cgcctcgttc
     4381 caggataggt ttgcaccggg aaaatgagct ttttccaacg gcgtcgaata cgaatttata
     4441 tttctcctcc gggcgggtaa aatcctcatt gatataatcg atcactttta ccgcaccgat
     4501 cgattttacc aaatcttcat cctttttccg gcaaaccgcc gtcacctttg ccccgtaata
     4561 gttcagaagc tgaaccgcag ctgaaccgat agccccggtt gcaccgttga caagcacttt
     4621 ttgccctttt ttaatatcca gtttattcag aaaattaagg gcatagtgag caccttctat
     4681 gccggcggcg gcgtgttcat aagtaatatg atccggaatc gtggctatag cattattttc
     4741 ggataatgtg agatattgcg cgtgcgagct taatccgaca tcatcaaacc caaaaacacg
     4801 atcgccttct ctgaattttg tgacattttt gccggctgat acaatttcac ctgcaaaatc
     4861 agttccctgt accgaccgct tcgggtggat gactccggtg aaaagcctga agatcaatgg
     4921 tttcccactc agtactgcac aatcggtccg gtttaccgtg gttgcaaata ccttaatcag
     4981 cacttcatgg tttttcggaa cgggcttttt aacagtttca atccggagaa tatccggaga
     5041 tccgtattgg tctgatactg ctgctaacat ggtcattctt acgatatttt aataattccg
     5101 ttgctgaatt taaatcagat caaacccatt tcctttgctt ttgcaacagc ccgggagcgg
     5161 ctatccacac caagtttgat gaatatattt ttcagatggg tttttaccgt attcagggaa
     5221 acaaacaaac gatccgccat ttcctggttc attaaatcct ctgccattag cttcatcatt
     5281 tccagttcac gtgcgctcaa agccgattcg gccaggccct tcttccggtt atcttttctt
     5341 tccagtgcca gtttcaggtt gctgatgaac tttttcggaa tatgaggtga tgctgatgtt
     5401 ccggcattga atattttttt gatgagcgca tcaataggat cattattaaa gacgaaaaat
     5461 gaaagcaagt tttcttccgc ggccagcgcc atggcttcgg ccaggacagc cacggctttt
     5521 tcatactcac cgcgcttttt gtacagaatg gcatgggcgg tttttaaatc gatgattctt
     5581 tcagccctgt ttccggctat agccagcggg cacaaagccg ataaaagtaa ttcggcttca
     5641 tccagttgat tttgcgccag taacaaccgg agatacgacg aataggccgc ttcattggca
     5701 aatgttttct cttgatcaag atccagccca aaaccggatg cagcatcccg ggccttatcc
     5761 atctgatttt tgtcgaggaa cataaaaatc ttccatccca tgtaataagc attcataaag
     5821 ggcggaagct tttctatgtt cataatgtcg tcaagctcgg cgattcttct ttctgcttca
     5881 gcctctcccc ggtcgcgaag aataaccgaa taaaccatta aaatcatcgc ttttaataaa
     5941 ctgtctttgc cggatttgca aagatcatag gcgattttaa tattgtcgaa ggccttgtca
     6001 ttctccgccc acataaagta cgaagtgcct aaaatgtaat acagtgaggc aaaagtccag
     6061 tctgctttgg tgatttgaga atagccgcga gctttcagaa gcgtaagcag ctccatgcat
     6121 tttttatagg ctgaccggta gtggcccagc tgctgctcgt tttcagccat acggatagcg
     6181 gtggttgaga tcaggtaaat gttcccggat tttttcgcaa agtcgaacgc tttcaaaaag
     6241 gccgcattgc tacgctccag gtcacctttc gaataccagg caatcccttc cgaaaaccag
     6301 gcccagctaa accattgcgg atcatcttcc ggaaggtatt ccatggccct cttactgtac
     6361 gcaaaaattt tttccgaatg ggtgtcatgg gatttcagat aagtgtaggc tactgcaatc
     6421 tttccggata gttttttata tccctgagct tcctttaaag caatatctct gcttttcagt
     6481 ttctcattga ccatccagtc ggcgcatgcc agatatggtt cagcatggcc gatctggccg
     6541 gatgtgatca atacccatgc gtaataaaag cagaagtcag cattgcgttg aatccattca
     6601 tcgggtaaaa tcgcaccata tcttaaaata gcggcatgct ttccattgca ccacatttcc
     6661 tcaaccacct tgctgatcag ttgaaggctc ttttcataat tctggccggc cagtgcattt
     6721 tcaatggcca aatcggtcat ttggtgctgt tcaaaccaat cgcaagcttt ggtgcgaata
     6781 tccgcagcat tcgtaatcat ctgctgctga aatcgttgct ttaacaaatc ggaaaacaaa
     6841 tgatgatacc ggtaccagtt tctttccgaa tccaaaggaa taacaaacat gttattcctt
     6901 tccaggtgtt caatgatgaa acggctgtcg ttcctgttca gaacccaatc acataacgaa
     6961 gcgttcatct gatccagcag cgatgttttc aacaagaatt ccctgatgtc gtccggttgt
     7021 gttttcagta cctcctccat gagataatcc atgatgtact ggttctcgcc ctgcaaatcc
     7081 cggatgaatt ccgacacatc tctgcgttgc tgcaccgata aagccgctag ctgtaacccg
     7141 gcaatccagc cttctgttct ggattccagc aaataaacat cttcattgga taatcccagt
     7201 ttcagctttt tattgaacag ggcggaaata tcgtgtgccg taaaactcag atcggccaaa
     7261 cgtagttcga ccatctgatg ctggcttctg atccttgcca gaggtaaagc ggggtccgac
     7321 cgggtcagga tcacaagatg aatgtttcct ggaatatatt gcaggataaa gttgattaat
     7381 tcaacgactt cgcggctgct gacatggtgg aaatcgtcga ggaccagtat aaagtcatgt
     7441 ttgatgcgga gaatatcgtt gatcagaagg gtggcaatgg cttcgggaga tggcttattt
     7501 ggcgactgca acaacctaag ggcggactgt ccgaattctt tccggacacc ttgtattcct
     7561 gaaataatat agcttaagaa ctcttccgga tcattgtcgc ctttatcgat ggaaaaccat
     7621 gcggcaggga tcttttgctg atggatccag tcgcatacca gggttgtttt gccaaaaccg
     7681 gcaggtgcgg agatcaggat cagcttacgg ttcagcccgc aattaagctt gtcaaacaga
     7741 tcttcccggt gaaccgtttg atcacccgga gaggggatat gtagttttgt cagaagcata
     7801 ggctcattaa tctttccagg gtgatggccc atcgtcagtc tttcaggttc agggcggctt
     7861 taaatttctc acggtagccc gcaccgatgg gaatacgctg ggcgcctatt ttaatgatcg
     7921 cccgctcgat gctttcaatt ttattgatgg ctacaataaa ggatttgtgg acccgaataa
     7981 aattatcggc cggcaactgg ctttccatcg tttgaaaatt ctgaagggtc ataatcctgt
     8041 ttttgggcgt tacgatcctc agatatgctc cctggccttc aatatacagg atatcagcaa
     8101 aattaatgcg ttccatccgg tattccgtct tcacaaacat ataatcttta gaaggtttat
     8161 gagccggatc aaccttaatt ttatcgatcg ctttcaggaa ccgctcaaac gaatagggct
     8221 tcagtaaata atcggtgacc gaataatcaa atccgctgac cgcatattgg tcatacgccg
     8281 atacaatgat gatctgaggc ggactccgga gagactcaag aaattgtaaa cccgaaaatt
     8341 gttccatctg aatgtcaaga aaaaccaggt caaccgggtt gccttttata aatgaaatgg
     8401 catccagtgc gttgttgaac gatgccaata aattcaacag cgggacctgg cggataaacc
     8461 cctccagctt ttcaacggcc agaggggtat cgtcgatgac aatgcaatta acggccatta
     8521 atttttacgg ttaaattcac cttaaatgta ctgttttcct gaatgaaatt taaaaaatgc
     8581 gagtcgggat aaagcagttc gagcctgcgt tttatatttt tcaggccgat gccgcccgca
     8641 tgatccctgg atattttctg ttcctcgttg aaaatattgc tgctctcaaa aattacggtg
     8701 ttaccttcaa tccggaacga gaaacgaatg gcgttccggg tgtttttatc ggtacaatgt
     8761 ttaaaggcat tctccacaaa agggataaaa agcatgggcg caatttgaat gcggtcagta
     8821 tctccggaaa cgttgaaaga taccagttcc ctgtttgaga attgtatttt ctgcaaatcg
     8881 atatagtctt caatatgctt gatttcaaga gataacggca ccctgccggc attcgtgtca
     8941 taaatcatat accgcatgat gccggagagt ttgatcaatg tttcggatgc ctgatcggca
     9001 ttggttttta tcaggctgtc gatattgttc agcgtattga agagaaaatg cggactgatc
     9061 tggtttttaa gcagcgccag ttcactctta aggttttcct gcgttcgcct ttcatgggtg
     9121 atccagactt ccatctttct gaaaaggagg ccaaacgtgg caaaggggat ggcaaaaagg
     9181 ccggtcatga ccaggaaata cgggaactca aaaccaaccc ggatggtcag atatttaaag
     9241 attgttgtaa taataaatgg aaggataata atcgtcagta atttaaagat ctgccctttt
     9301 aaattgtggg gttttagcag gaaatagcag gcgtaaaata ttcccacggg gaggaggatg
     9361 taaaggtaga agttgaaaat gatcccgaac aggttggaac ctgaaagaaa gtaggcaccc
     9421 atatccgggg gctcggttaa aaaacggaag ctgtctgata ataaagacca gaagcttact
     9481 tgatctgttc ccatcatata gctgaaggtg ccaaattcac gggacccaac ggagataacc
     9541 ctaaaaatcg ggaaaatcat ccagaaaccg gcatgcaata aaattttcac tgaattcttc
     9601 atggttgaaa atgattaaag ttaaacatga agcaaaattc atcggaaata taaggaataa
     9661 aaaatccgtt taacaaacgc ggcctgttta ttcatgaaat gacgggattt atgattaagg
     9721 gccggaagcg ccattacaca gcaaaaactt ctgattcgcc gaaacgggaa gcaatggtta
     9781 cgcgggtgcc gttggcatga ggagcaaata aatccgttac gatctgcgga tggttatttt
     9841 gccgggagag gtgtgaaagc accagaagct gcatgaacgg ggcgcgataa ctgttaaaga
     9901 gctccagcgc ctgcaggttt gaaaggtgcc cattgccccc gcggattcgt cttttcaaaa
     9961 agtacggata gcggccttct tcgagcatcg tctcatcgta attcgcttca aggaaagctg
    10021 cgtgacaccg gctgaagtga tggatcagct gttcgcatac cgaaccgata tcggtaaaaa
    10081 tgcccactgt gataccgctt cctgtcacgg tgaagctatg cggatcggct gcatcgtgat
    10141 gtttttgaaa ggctttaacg gtaaggctgc cgaaatgcaa ttcctcaccg gcggaaaacg
    10201 gctgcaaaag ttgcggttca agccggatca ggctgttccg gtgagtggca ggagtgatac
    10261 agaccgggat cctgtgcttc cgggaaagca cctccacgcc cctggtatgg tcagaatgtt
    10321 catgggagat aaaaatggcc ctgacttttg tcaaggacag gccctgtcgt ttcagccgtt
    10381 gttctgtctc cctgcacgag atgcccgcgt cgataaggac ggcatcatgg tcgttgccaa
    10441 catagtagca atttccattg cttccggaat tgagcgatga ga
//
LOCUS       DAOPNH010000095        10482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6840_length_10482_cov_7.021483, whole genome shotgun sequence.
ACCESSION   DAOPNH010000095 DAOPNH010000000
VERSION     DAOPNH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6840_length_10482_cov_7.021483"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="PKZ74_06500"
     CDS             <1..1838
                     /locus_tag="PKZ74_06500"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPM92445.1"
                     /translation="KVVGDIYTLGSYAIRPSFRYKKRYKYTGSASFTYAINITGIKEA
                     PDYSRKRDFSIRWNHRQDPKARPKSTFSADVNIVSSTLNRYNPSTTNDYLSNTFRSSI
                     AYQTNFAGKYFLTVNASHDQNTLSKIVNVTLPEVSFSVNRFNPFVTKKKTGPNKWYHN
                     ITMSYNMNARNTVSAADSVILTTAALDLMKNGIKHYMPITSPVKVLKYFNWSNSITMT
                     DRMYFESYRKYWSNETIIDGNDTITGFMVQDTLNGFNNVFDFSFSSSLSTKVYGLVQF
                     GRKFPVRAIRHVLTPSVSFSYRPDFSTDFWGYFDSYIDKDGKEKVYSKYEGAIFGAPP
                     MGESGAIGFSFANNLEMKVRSRKDTITGTKKVSLIDNFTIGFSYDFARDSLNLSTILM
                     QGRTRLFKNLDIGYRADLDPYVLDSAGKNLNRFEWDVNRRLLRLKQTNWSVSLNWRLN
                     SKELGKNKEKPGGTPVTTGAPQVPTGDESEMTEIADNPDQYIDWSIPWDLTLSYSFNY
                     TVVRRYPEYILYKDKKIVQTLGVSGNISVTPKWKIGFMTGWDFEDKDLSYTSISIYRD
                     LHCWEMRFNWIPTGYQKSWNFQINAKASLLQDLKLNKKKDFRDNF"
     gene            1890..2270
                     /locus_tag="PKZ74_06505"
     CDS             1890..2270
                     /locus_tag="PKZ74_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM92446.1"
                     /translation="MKKIISTPDAPKAVGPYSQAVEFNGMLFISGQIPIDPAIGKIVD
                     GGIREQTEQVMKNIGVILKEAGYDYKDVVKSTCLLNDMDNFAAMNEVYARYYPENPPA
                     RAAYGVVRLPLGAMVEIETIAMKK"
     gene            2298..4439
                     /locus_tag="PKZ74_06510"
     CDS             2298..4439
                     /locus_tag="PKZ74_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPM92447.1"
                     /translation="MKKCIITLLLGFLLSNLSIADEGMWIPSLIGQQKLDDMKKRGLK
                     LSAEDIYSINQSSLKDAVVLFGRGCTGAIVSAEGLLLTNHHCGFGSIQRHSAVEHDYL
                     RDGFWAMSREEELPNAGLSVTLLVRMEDVTDRVLASVKEGMSEADRQKAIDDAAKAIV
                     EEAVKETHYQAEVEPLFNGNQYFLYVSEVFKDVRLVGAPPSAVGNFGGDTDNWMWPRH
                     TGDFSVFRIYADSNNIPAEYSKNNKPYQPKKYFEVSLDGVDKGDFTMVYGYPARTQQY
                     LPSYMVELIAEQSNPEKIAIRREKLDIMGAAMNSDQKIRIQYASKYAGVANAWKKWMG
                     ESKGLQRFDAVEKKQAFEKDFSAWASMNAPQYSGILPAYQALTDELTPCQLWVDRFSE
                     AVWSLDIIRLAAGYRNLAGMDKTAGEDLRKEADRLKGNLNGFFKDYDQATDKKLFAAM
                     LGLFRNNSQPQELPGIYSEIDRKFGTDINTYANWVYENSLFTDQERMGEFLDGYSASK
                     AKKIAADPVYKVMISFFDKFIADYSFRYQKLMTGQDSLQRVYMKAIMEMKSNRLLFAD
                     ANFTLRVSYGVVKDYFPKDGVYYDYQTTLEGIMEKDDPDIYDYRVPEKLKELYRAKDY
                     GRYGDNGKMNICFTAANHTTGGNSGSPVLNAEGQLIGLNFDRNWEGTMSDIMYDPDMC
                     RNIVLDIRYCLFIMDKYAGAGHLVEEMEIAD"
     gene            4543..6357
                     /locus_tag="PKZ74_06515"
     CDS             4543..6357
                     /locus_tag="PKZ74_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPM92448.1"
                     /translation="MRRHLPASRWSLVALRWSLISLSLFLNFNQLSAQSIHFEQNSYY
                     HAFGEQKTPFYDSLNGFIGLKQNVKTDACQLHRVVFGFHPYWSGSSYLNYQWNLLSDL
                     CYFSYEVDPVSGDPVTIHDWLTDPAIDSAQANGVRVHLCATIFYGHSVFFNNPAARQN
                     LIDNLISLVQERNADGVNMDIEAVPSSVSDSMTVFMQDLSIQLKAAIPGAILSIDLPA
                     VDWSNNFQIDELSDYLNWFFVMGYDYYWNGSSEAGPVSPLYSLTPGYDYSLARTVSAY
                     EKAGLAPEKFILGIPYYGRQWKTASDIIPSPVLSNGIALTYSNVRNNSVIYNPEVYRW
                     EPASASSCYVFFLNGYWNQCFIGLDRDLREKYDIANYRNLAGIGIWALGYDNGYPDLW
                     QAISDKFTDCYIPPVYDTIFDSGGQAWNYYPFEDYEMTIDHGYQDPRFLTFTTCSLEA
                     GYDSLWIYSGSDSGMVFIGSYSGNENPGTITSADGSFTLRFRSDGLTQAPGWQAIFHD
                     GTIGIESTVKTRDRLLIYPIPAKDEIIIELPKDERFTGVSINDLSGRTLISAKRLHQP
                     AGSTMRISIGNWPAGTYFVTLAGDRGRGITGMFVKVAE"
     gene            6556..8439
                     /locus_tag="PKZ74_06520"
     CDS             6556..8439
                     /locus_tag="PKZ74_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPM92449.1"
                     /translation="MEDNKVKEKLFPEFPPVTVADWEKKINQDLKGADYEKKLVYRPA
                     EGIRIKPYYAADDLLSLEYLNQFPDSFPYIRGNKKSGNDWFIRQDIVVTDLKRANEKA
                     LDILMKGIGSLGFILDDEKEYSKDDLDLLLKNIFAEMVEINFTAGRHAESLMKNHYEM
                     LVRYNRDFGKIYGSLEFDPFGRLATKGKFYLTEEEDLEICSELIRISGHLPHFTVIAV
                     GGDHFHNAGATIVEELAFSLASGAEYLTRLTEKGLSVNQVAPKIRFRFATGSNFFMEI
                     AKYRAARQLWAHIVKAYGPSREEVSRMTVHATTSKWNKAVYDPFVNMLRTTTESMSSV
                     VAGIDSLTVGPFNAIYQQYDDFAERIARNQQLLLKEESYLDQVADPSAGSYYIESMTD
                     SIAAEAWKLFLEVDSVGGYLQAVKSGFIRERIKATAEKRDAGLANRKDVILGVNQYPN
                     FTEKKEEPLAEQVFSPARSGETDLITEPLRLYRGAQPFEALRYKTDMYALKHKRPAAF
                     MFTYGNLAMRIARSQFSRNFFACAGFDTIDNLGFKTIDEGIRAFHESRAEILVICSAD
                     EEYPVIAPAILEALKDKAKVVIAGYPKDSVDELKAKGIEHFIHVRSNVLEELKKFQAL
                     LGL"
     gene            8591..>10482
                     /gene="scpA"
                     /locus_tag="PKZ74_06525"
     CDS             8591..>10482
                     /gene="scpA"
                     /locus_tag="PKZ74_06525"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPM92450.1"
                     /translation="MKPNFQKINIKSVAAQRISAAEWEQQNRKEEDWLTSEGIPVKHA
                     YTAEDLAGMEHLSYAAGLPPFLRGPYSTMYVQKPWTIRQYAGFSTAEESNAFYRRNLA
                     SGQMGLSVAFDLATHRGYDSDHERVVGDVGKAGVAIDSILDMKVLFDQIPLDRMSVSM
                     TMNGAVLPIMAFYIVAGLEQGARLEQLSGTIQNDILKEFMVRNTYIYPPLPSMRIIAD
                     IFKYTSAHMPKFNSISVSGYHMQEAGATAELELAYTLADGWEYLRTGTQAGLDIDDFA
                     PRISFFWGIGMNHFMEVAKMRAGRMLWAKIVNKFNPKNPKSMMLRTHCQTSGWSLTEQ
                     DPFNNVARTCVEALGAVLGGTQSLHTNALDEAIALPTDFSARIARNTQIFIQEETQVC
                     RTVDPWAGSYYIEYLTDQIARKAWALIEEVEALGGMAKAIETGIPKMRIEEAAARKQA
                     RIDSHKDTIVGVNKYRLEKEAPIETLEVDNTAVRESQVKRLHLLKSQRNQADVDRILE
                     AITRCCETGEGNLLELSVEAAKARATLGEISNACEKVFGRYKAVIRSISGVYSAESKS
                     DESFRRACELADKFASLDGRRPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLF
                     QTPVETA"
BASE COUNT         2735 a         2648 c         2744 g         2355 t
ORIGIN      
        1 ttaaagttgt cggggatatc tatacactgg gtagttatgc catcaggccc agcttccggt
       61 ataaaaaacg gtacaaatac accggatcgg ccagctttac atacgctatc aatatcacgg
      121 ggataaagga agctcccgat tacagccgta aacgcgactt ttccatacgc tggaaccacc
      181 ggcaggaccc gaaagcccgg ccgaaaagca ctttcagcgc cgatgttaac atcgtttctt
      241 ccaccctgaa ccgctataat ccttcgacga ccaatgatta tctttcgaat accttccggt
      301 cgagcatagc ctaccagacc aacttcgccg ggaaatactt cctgacggtc aacgcctctc
      361 acgatcagaa taccctgtca aaaatcgtga acgtaaccct gccggaagtc tccttctcgg
      421 tgaaccggtt caatcctttc gtcaccaaaa agaagaccgg accgaacaaa tggtatcaca
      481 acatcacgat gagctacaac atgaatgccc ggaatacagt ttcggcggcc gattcggtca
      541 tcctgacgac ggcggcgctc gacctgatga aaaacgggat caaacattat atgccgatca
      601 ccagcccggt gaaggtgctg aaatatttca actggagcaa ctcgatcacg atgaccgaca
      661 ggatgtactt cgagtcctac cggaaatact ggtcgaacga aacgatcatt gatggaaatg
      721 ataccatcac ggggtttatg gttcaggata ccctcaacgg gttcaacaat gtgtttgatt
      781 tcagtttttc atcttctctc agcacaaagg tttacggact ggtgcagttt gggcggaagt
      841 tcccggtcag ggcaatacgg catgtgctta ccccctcggt ctctttttct taccggcccg
      901 atttcagtac cgatttctgg ggttattttg acagttatat tgataaagac ggaaaggaga
      961 aagtttattc caaatatgaa ggggcgatat tcggggctcc gccgatgggt gaatcgggcg
     1021 cgatcgggtt cagttttgcc aataacctgg agatgaaggt gagatccagg aaagatacta
     1081 tcaccggtac aaaaaaggtc agcctgatcg ataatttcac catcggattt tcgtacgatt
     1141 ttgccaggga ttcactcaac ctgtcgacta tactgatgca gggaaggacc cggcttttca
     1201 agaatcttga catcggctac cgtgccgatc ttgaccccta tgttctcgat tcggcgggga
     1261 agaacctaaa ccggtttgaa tgggacgtca accggcgact gctcaggctg aagcagacca
     1321 attggtcggt cagtctcaac tggcggctga attcgaaaga actgggcaag aacaaggaaa
     1381 aacctggtgg aacaccggtt acaacaggag ccccgcaggt tccgaccggg gatgagagtg
     1441 aaatgacgga gatcgccgat aatccagacc agtacatcga ttggtcgatc ccgtgggacc
     1501 tgaccctgag ctattctttc aattatacgg tggtgcggag atatcccgaa tacatcctgt
     1561 acaaggataa aaagatcgtc cagacactgg gagtatcagg caatatcagc gttacaccga
     1621 agtggaagat cgggtttatg accggttggg attttgaaga taaagacctg tcgtatactt
     1681 caatcagcat ctaccgcgac cttcattgct gggagatgcg tttcaactgg atccccacag
     1741 gttaccagaa gagctggaac ttccagatca acgccaaagc ctctttgctg caagacctta
     1801 aactcaacaa gaaaaaggat ttcagggata atttctaatt ctgaatgaac ctgaatattt
     1861 ataaaataaa acattaaaaa tactacatta tgaaaaaaat catcagtact cccgacgctc
     1921 cgaaagccgt aggaccgtat agccaggctg tagaattcaa cggaatgtta tttatttccg
     1981 gccagatccc gatcgatccg gccatcggga agatcgtcga cggagggatc agggaacaaa
     2041 ccgaacaggt aatgaaaaat attggcgtga tcctgaaaga agccggttac gattacaaag
     2101 atgtcgtcaa atcaacctgc ctgctgaacg atatggacaa tttcgctgcc atgaatgaag
     2161 tgtatgcccg gtattatccc gaaaacccgc cggcccgggc agcgtatggc gtggtccgcc
     2221 tgcctctcgg agccatggtg gagatcgaga cgattgcaat gaaaaaataa acattaagaa
     2281 ttaaaaatta ggaattaatg aaaaagtgta tcataactct tctgttaggt ttcttgctct
     2341 ctaacctgtc aattgccgat gaaggaatgt ggattcccag cctgatcggg cagcagaagc
     2401 tggacgatat gaagaaacgg ggcctgaagc tttccgccga ggatatttac agcatcaacc
     2461 agtccagttt gaaagatgcc gtggtgctct tcggtcgcgg ttgcaccggg gcgatcgtat
     2521 ccgctgaagg cctgctgctc accaaccacc attgcggttt cggcagcatc cagcgccaca
     2581 gcgcagtgga gcacgactat ttgcgcgacg gattctgggc gatgagccgc gaagaagagc
     2641 tgcccaatgc aggactctcc gtaactctgc tggtcaggat ggaggacgta acggatcgtg
     2701 tcctggcatc ggttaaagag gggatgagcg aggccgaccg gcagaaagcc atcgatgatg
     2761 cagccaaggc gattgtggag gaagcggtca aagaaacaca ctaccaggcc gaggtcgaac
     2821 cgctgttcaa cggaaatcag tattttctct atgtttcgga ggttttcaag gatgtccgcc
     2881 tggtaggggc acccccgtcg gcagtgggca attttggcgg cgataccgat aactggatgt
     2941 ggccgaggca tacgggcgac ttcagcgtct tccggattta tgccgacagc aacaatatac
     3001 ccgcggagta ttcgaagaat aacaagcctt atcagccaaa aaagtatttt gaagtttcac
     3061 tggatggcgt cgataagggt gattttacga tggtctacgg atatcccgcc cggacacagc
     3121 aatatctgcc atcttacatg gttgaactga tagccgaaca atcgaatccg gagaagatag
     3181 ccatacgtcg tgaaaagctc gacatcatgg gagctgcaat gaactccgac cagaagatca
     3241 ggattcagta tgccagcaaa tatgccgggg tggcgaatgc ctggaagaag tggatgggtg
     3301 aaagcaaagg gttgcaacgt ttcgatgccg tagagaaaaa acaggcattt gagaaggatt
     3361 tttcggcctg ggccagtatg aacgcaccgc agtacagcgg gatccttcct gcttaccagg
     3421 cgctgacgga tgaactgaca ccctgccagc tctgggtcga ccgtttttca gaagccgtat
     3481 ggagcctcga tatcatccgc ctggcagccg gctacaggaa cctggccgga atggacaaaa
     3541 cagccggcga agacctgcgg aaagaagcgg accggcttaa aggcaatctg aacggcttct
     3601 ttaaggatta tgatcaggca acggataaga agctctttgc cgccatgctc gggcttttcc
     3661 ggaataattc gcaaccgcag gaactgccgg gcatttacag cgaaatcgac aggaagttcg
     3721 gcaccgatat caacacttac gcaaactggg tttatgagaa ttctttattt acggaccagg
     3781 aacgtatggg cgaattcctg gatggttatt ccgcctctaa agcgaagaaa atagcggccg
     3841 atccggtgta caaagtgatg atcagtttct ttgacaagtt catagccgat tattcgttca
     3901 gatatcagaa attgatgaca ggacaggata gtctgcagag ggtttatatg aaagccatca
     3961 tggagatgaa aagcaacagg ctgttgtttg ccgacgccaa tttcaccctt cgggtttctt
     4021 atggcgtggt gaaggattat tttccgaaag acggagtata ttatgattac cagacaaccc
     4081 tggaagggat catggaaaaa gacgatccgg atatttacga ttacagggtc ccggagaaac
     4141 tcaaggagct ttaccgggca aaggattacg gccggtatgg cgataacggg aagatgaata
     4201 tctgtttcac ggcagccaat cacactacgg gaggcaattc cggcagcccg gtcctgaatg
     4261 cggaagggca gctgatcggc ctgaatttcg accggaactg ggaaggaacg atgagcgata
     4321 tcatgtacga tcccgacatg tgccggaaca ttgtgctcga catccgctac tgcctgttta
     4381 tcatggataa gtatgccgga gcgggccatc tggtggaaga gatggaaata gcggattagt
     4441 tcaaagttca aagatgtagt gtgggttgga ttttataaat ttccacaggg tgtggtagtt
     4501 ccttttgcaa tattttctgg atcgagatgg agtctcgaaa tgttgagacg ccatctgcct
     4561 gcatcgagat ggagtctcgt ggctttgaga tggagtctca ttagcctgtc cctgtttctt
     4621 aacttcaacc aactttccgc ccaatccatc catttcgagc aaaatagcta ttatcatgcg
     4681 ttcggagagc aaaaaacccc attttacgat tcacttaatg gtttcatcgg gctcaaacag
     4741 aatgtcaaga ccgatgcatg ccagctgcat cgtgtcgtct ttggcttcca tccctactgg
     4801 tcgggctcat catacctgaa ttaccaatgg aaccttttat ccgatctttg ctacttttcg
     4861 tatgaagtcg atcccgtttc gggcgatccg gtgaccatac acgactggct caccgatccg
     4921 gctatcgact ctgcacaggc caatggcgtc agggttcacc tttgtgccac gatcttctac
     4981 gggcattcgg tattctttaa caaccctgct gcccgtcaga acctgatcga taacctcatc
     5041 tcgctggtac aggagcgtaa tgctgacggc gtaaacatgg acatcgaggc agtaccctcg
     5101 tcggtgagcg acagtatgac ggtttttatg caggacctct ccatacaact caaagcagca
     5161 atccccggcg ccatcctgag catcgacctt ccggccgtcg actggagcaa caattttcaa
     5221 attgacgaat tatcagatta tctcaattgg ttctttgtaa tggggtacga ttattactgg
     5281 aacgggagtt cggaggcagg acccgtctcc ccgctctatt cgctcacacc cggctatgac
     5341 tacagtttgg cacgaaccgt ttcggcttac gaaaaggctg gcttggcgcc ggaaaagttc
     5401 atcctcggaa ttccctatta tggcaggcaa tggaaaaccg cttcggatat tattccttcc
     5461 ccggttctat caaatggcat cgccctgacc tattccaatg tcaggaataa ctccgttata
     5521 tataacccgg aggtctatcg ctgggaaccg gccagcgctt cttcctgtta tgttttcttc
     5581 ctgaacggat actggaacca gtgttttatc ggccttgacc gcgacttgag ggagaaatac
     5641 gatatcgcca attaccgcaa cctggccgga atcggcatct gggccttggg gtatgataac
     5701 ggatatccgg acctgtggca ggccatttcc gacaaattca ccgattgtta tattccgccg
     5761 gtatacgata caattttcga cagtggaggc caagcctgga attactaccc gttcgaagat
     5821 tatgaaatga ccatcgatca cggataccag gacccgcggt ttctgacttt caccacttgc
     5881 agcctggaag ccggatacga ttccctgtgg atttattcag gaagtgattc cggcatggtt
     5941 ttcatcggaa gttattccgg aaacgagaat ccgggaacga ttacctccgc cgatggctcc
     6001 tttaccctga gattccggtc ggatggactg acccaggcgc ccggctggca agccatattt
     6061 cacgacggga cgattggaat agaatcaacg gtgaaaaccc gcgatcggct gctaatctat
     6121 cccattccgg caaaagatga gattatcata gaacttccga aggatgaacg gtttactgga
     6181 gtttcgataa atgatctttc aggaagaacg ctcatcagtg cgaagagatt acatcaaccg
     6241 gccggtagca ccatgagaat cagcatcggg aattggccgg cgggcactta ctttgttacg
     6301 ctggccggcg acagggggag aggtattacc gggatgtttg tgaaggtggc ggaatgagtc
     6361 aaaaagggga cgccatccgc cggctgtcgg atggagtccc tcgggacgcc atccctaatc
     6421 cttgttaatt tattcacaat aaaccctccc ttgaattatt gttcgtaaat ttgcgcccgg
     6481 ccatagatgg agccatcgtt tatcgctcga ataaaaagat aaatgattga taaacaagat
     6541 ttagacagac atagaatgga agataataaa gtgaaagaga aactcttccc cgagtttccg
     6601 cccgtgacgg tcgccgactg ggagaaaaaa atcaatcagg acctgaaagg ggccgactat
     6661 gaaaaaaaac tggtttaccg acctgcggaa ggcatccgga tcaaacccta ttatgccgcc
     6721 gatgacctgc tatccctcga ataccttaac cagttccccg attcgttccc atacatccgc
     6781 gggaataaaa aatcgggaaa cgactggttt atccgccagg atatcgtggt gaccgacctt
     6841 aaaagggcca atgaaaaagc gctcgatatc cttatgaaag ggatcggctc cctcggattt
     6901 atcctggatg atgaaaaaga atattccaaa gatgatctcg acctgctgct gaagaatatt
     6961 ttcgccgaaa tggtcgagat caattttacc gccggccggc atgccgaatc gttaatgaaa
     7021 aaccattacg aaatgctggt ccggtacaac cgtgatttcg gtaagatcta cggttctctg
     7081 gagtttgatc cgttcggccg gctggccaca aagggcaaat tctacctgac agaggaggaa
     7141 gacctggaga tttgcagcga actgatccgg atttccggac acctgcctca ttttaccgtt
     7201 attgcggtcg gtggcgacca tttccacaac gccggcgcta ccatcgtaga agagctcgct
     7261 ttcagcctgg cgtcaggggc agagtacctc acacgcctta cggaaaaagg attgtcggtg
     7321 aaccaggtcg cccccaagat caggttccgt tttgccaccg gatccaattt ctttatggag
     7381 atcgccaaat accgcgctgc ccgccagctt tgggcacata tcgtcaaagc ctacggacca
     7441 agccgtgaag aggtttcccg gatgacggtt cacgctacta cctccaaatg gaacaaggcg
     7501 gtttacgatc cgtttgtgaa tatgctgcgg acaacaacgg aatcgatgtc gtccgtcgtg
     7561 gcggggatcg attcgctgac ggtcggtcct tttaacgcga tctaccagca gtatgacgat
     7621 tttgccgaac ggatcgcccg caaccagcaa ctcctgctga aagaagaatc gtatctcgac
     7681 caggtggccg atccgtcagc cggttcctac tacatcgaaa gcatgaccga cagcatcgcc
     7741 gccgaggcct ggaagctgtt cctggaagtg gattcagtgg gcggatacct gcaggcggtt
     7801 aaatccggat ttatccggga aaggataaaa gccacggcgg aaaaaagaga cgccggcctg
     7861 gccaaccgga aagatgtcat tctcggagtc aaccagtacc cgaactttac cgagaaaaaa
     7921 gaagagccct tggcggaaca ggtcttcagc ccggccagat ccggcgaaac ggacctgatc
     7981 acggaaccac tccgtcttta ccgcggcgcg cagcctttcg aagcgctacg gtacaaaaca
     8041 gacatgtatg cccttaaaca caaacggccg gccgccttta tgttcaccta cggcaacctg
     8101 gccatgcgga tcgcccgctc gcagttcagc cgcaacttct tcgcctgcgc cggttttgat
     8161 acgatcgata acctgggatt taaaaccatc gatgagggta tccgggcttt ccacgaaagc
     8221 cgcgccgaga ttctcgtcat ttgcagcgcc gatgaagaat acccggtgat cgccccggcg
     8281 attcttgaag ccttgaaaga taaagcaaaa gttgtgattg ccggataccc gaaagattca
     8341 gttgacgaac tgaaagcaaa agggattgag cattttatcc acgtccggtc gaatgtgctc
     8401 gaagagttga agaagtttca ggctttgctt gggttataag atttactttc aaaaaatatt
     8461 ttattatgat ttaccacaaa ggcgcgcaaa ggattacaca aaggcgcacg aaggggttca
     8521 gtatttctgg gatcctttgt gttcttcgtg aaatcctttg tgttcctttg tggttataaa
     8581 ataaaaaatc atgaaaccga attttcagaa gatcaatata aaatcagtcg cggctcagag
     8641 aatctcagct gccgaatggg aacagcagaa ccgcaaagaa gaagactggc tcacctccga
     8701 aggtatcccg gtgaagcatg cttacacagc cgaagacctc gccgggatgg agcacctgag
     8761 ctatgccgcc ggcctgccgc cgttcctgcg cggaccttat tcgacgatgt acgtccagaa
     8821 accctggacc atcaggcagt atgccgggtt ttccaccgcc gaggaatcca atgcctttta
     8881 ccgccgcaac ctggcctcgg gccagatggg attgtcggtg gcatttgatt tagccaccca
     8941 ccgcggctac gattccgacc acgagagggt cgtcggcgat gtgggcaaag ccggcgtggc
     9001 tatcgattcg atcctcgata tgaaggtcct gttcgaccag atcccgctcg accgcatgtc
     9061 ggtgtcgatg accatgaacg gcgcggtgct gcccatcatg gctttttaca ttgtggcagg
     9121 gctggagcag ggggcgaggc tggagcagct ctccggaacc atccagaacg acattctcaa
     9181 agagttcatg gtgcgcaaca catacatcta tccgccactg ccatccatgc gcatcatcgc
     9241 cgatatcttc aaatatacct ctgcccacat gccgaaattc aacagcatca gcgtgagcgg
     9301 gtaccacatg caggaggcgg gcgctactgc cgagctggag ctggcctaca cgctggccga
     9361 cggctgggaa tacctgcgga caggcaccca ggcagggctc gacatcgacg actttgcccc
     9421 gcgtatctcg ttcttctggg gcattggaat gaaccatttt atggaggtgg ccaaaatgcg
     9481 ggccgggcgc atgctctggg cgaaaatcgt caacaaattc aatccgaaga atcccaagtc
     9541 gatgatgttg cggacacact gccagacctc cggctggagc ctgacggagc aggatccgtt
     9601 caacaacgtg gcccgcacct gtgtggaggc cctgggcgcc gtgctgggcg gaacgcagtc
     9661 gctgcacacc aacgccctcg acgaggccat cgcattgccc accgattttt cggcccgtat
     9721 tgcccgcaat acgcagatct tcatccagga agagacacag gtctgccgca ccgtcgaccc
     9781 ctgggccgga tcgtattata tcgaatacct gaccgaccag atcgcccgga aagcctgggc
     9841 gctgatcgaa gaggtcgaag ccctgggcgg aatggccaaa gcgattgaaa caggaattcc
     9901 caagatgcgg atcgaggagg ccgctgcacg caagcaggcg aggattgact cgcacaaaga
     9961 cactatcgtg ggggttaata aataccggct cgaaaaggag gcgccgattg agacgctgga
    10021 ggtcgataat acggccgtca gggaatcaca ggtgaagaga ctccatctcc tcaaatcaca
    10081 gcgcaaccag gccgatgtcg accgcatcct cgaagccatt acccgctgtt gtgagaccgg
    10141 tgaaggcaat ttgctggagt tgtcggtcga ggcggccaaa gcccgggcca cgctcggcga
    10201 gatctccaat gcctgcgaga aggtgtttgg aagatataag gcggttattc gttcgatatc
    10261 aggcgtttac tccgccgagt cgaagagcga cgagagcttc cgccgggcct gcgagctggc
    10321 cgataaattt gcctcgctcg acggacgccg gccccggatc atgatcgcga agatgggcca
    10381 ggacggccac gaccgcggcg cgaaggtggt ggctaccggc tatgccgaca ttggtttcga
    10441 cgtcgatatc ggcccgttgt tccagacccc ggtggagacc gc
//
LOCUS       DAOPNH010000096        10481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6842_length_10481_cov_4.933244, whole genome shotgun sequence.
ACCESSION   DAOPNH010000096 DAOPNH010000000
VERSION     DAOPNH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6842_length_10481_cov_4.933244"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2643)
                     /locus_tag="PKZ74_06530"
     CDS             complement(46..2643)
                     /locus_tag="PKZ74_06530"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPM92451.1"
                     /translation="MKKTILHLLLLAAMLAIPGLSAGQDFLPVLNDNYMGVNQAFLQP
                     ASIADSRFKSDFNAGGFSSDIVSTGIRFKSRWLLDPTGILTNADWWDENTYLDKANGK
                     DKSMFMSQSAIGPSLMISMGSRHAFALTSRVRSITNVDGMDEPLFRMIYSNFSEKESL
                     YWNRWYYDDNMRAVQHIFGDYGLTYARVFYDQREHFIKAGVSLKLLQGIASSYLQTDQ
                     LYYYYDGAEPSGAKPISWNSPYVQGGLSDNWGYWRPDGSYAFDMNFQLTARPSFGMDF
                     GAVYEYRPRWMEHNNPKTGKPRADRNKYFIKVGISVVDLGRLKYVKDYHSSDLVTSFT
                     PDYVNRYNSSDNSVPVNTSWLNAGSVDFTFRKYVPFADTMYRRYSSGHDLGVVRAKDN
                     KEEFKVSLPTAFSIQADVYLIEGVYVNLTTYTALNQGYSKPAHNHYLTTVSVTPRYEQ
                     KWYGFALPIQVDQYGHFEAGLGVRAGIVYFGVSNLFSTVFEDPYGMKGYVGVKIPVPH
                     NDPNKTPKVKKAKGGNDVKIIVCCTCCGCAHDSVDCEKCKVYCPEISKTPFDPVHQEN
                     SINIMNSKIIIDKSVRTDSQINKTIEIKEQESDSTGRPCPPPPSMLHFDNNKSIINDT
                     SIVILDYFAECLLKDPSSVVLITGHTDSIGSDSANMILSYNRANSVKNYFIIRGVNPK
                     QINLKWHGERMPIAPNSTSPGRAMNRRVVLEITKDQIKIVTDPPRIPKETSPDTVERT
                     HPIPIPIPNIIPSAQPTYIDYVLFKSGQAIIDQKYYPELDKIAKYLESNPSESIRIEG
                     FSDNIGGTADNMKLSRFRANVVARYLISRGANADQITINWFGESNPPYDNSTQEGQAQ
                     NRRVNIIMI"
     gene            2645..3649
                     /locus_tag="PKZ74_06535"
     CDS             2645..3649
                     /locus_tag="PKZ74_06535"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92452.1"
                     /translation="MKLPLPIFNTHYKSMTALVLYSFTAGRFQEPASFFSFDSMQVEN
                     TLTLPATKKTVTLLIFNWMNNIFLRIITSVIFVSSVGLTEPVSAQDQPGLMRTAEDYS
                     SLITLNYQDTLLLNVNEEFDLPVIIKKQSKISAISLGFFYDPVYLEIDSMILAKGTSG
                     FFFSVNDSAFQMAWSSIQPLELEDGDTLLVLNMKTLDISDLTNTLRISLNETSELADT
                     AANPIEDVVLEIPEIGYLIPDHHDSIGESTICIYPNPFRDHATVFFTLKVGSEVKITV
                     NNSSGIEIFTLTDRPYEPGNHEIRLDKLRMPRGIYLLKFEIRNSDNSGQKVFKIISAF
                     "
     gene            complement(3661..5706)
                     /locus_tag="PKZ74_06540"
     CDS             complement(3661..5706)
                     /locus_tag="PKZ74_06540"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPM92453.1"
                     /translation="MSKPIVDLAADTIRILSAAMVEKANSGHPGGAMGGADFINILYS
                     EFLNFNPDDPAWPLRDRFFLDPGHMSPMLYSVLHLCGYYSTEDLKNFRQWKSITPGHP
                     ELDVLHGIENTSGPLGQGHAMAVGSAIAERFLAARFGEWMAHKTFAFISDGGIQEEIS
                     QGAGRIAGYLGLNNLVMFYDANNVQLSTKIGEVTSEDTAMKYRSWNWYVQTINGNNAM
                     EIREAISNALAEEQRPSLIIGKTIMGLGAVDSQGNRMEGLTSTHGQPLSKAGVSLSDT
                     IRKLGGDPDNPFEVSDEVKEYYRLILSKKRIYTKDRDDQMTAWKDDNPDQALLWNNYT
                     SGEVPDIDTEAIPIKPDAPTRNTSGDVLSYFSKSIGNMIVMSADLSNSDKTEGFLKHS
                     RPFQPGDLNGGFLHAGVSELTMAAIANGIALHGGLFVVCGTFFVFSDYMKPAIRLAAL
                     MRLPVKYVFTHDSFRVGEDGPTHQPVEHEMQLRLLEQLKNHRGHNSLLVLRPADSAET
                     IISWKLALENTETPTALILSRQNIKDIPAPAGGNRLKEAVHTRKGAYTVYEPDNEPDV
                     ILLANGSEVSTLIECAFLLKNDHNIQARVVSAPSEGLFQLQDEHYRKSIIPDNTPVFG
                     LTAGLPVTLKNLAGCKGKVFGLEHFGYSAPFQVLDEKFGFNAQNIKAMVLDFLQEPI"
     gene            complement(5803..6936)
                     /locus_tag="PKZ74_06545"
     CDS             complement(5803..6936)
                     /locus_tag="PKZ74_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HPM92454.1"
                     /translation="MKHPVIIIDDHIPFIKGVLEPFARVTYAPGGVISKDIASKADGL
                     IIRTRTRCNAELLDGIPVKFIASATIGTDHIDIQYCNNRSIEWYHAPGCNSGSVMQYM
                     ASAFSRLSTKYHISLAGKTIGIIGAGNVGKKIALLAEILGMSPLLNDPPRARNEGQQG
                     FVSLQKIKEECDLITFHVPLNLGGDDRTFHLANDAFFNSLAKKPLIINTSRGAVVDGS
                     ALKVAHQKGMIRGFVADVWEDEPHIDRELLAIADIATPHIAGYSVEGKANGTAAVVRA
                     ASRYFGFGIDEWYPENIPGPEKPVIEITSAGRSDERIIDEAILGTYDVLKDDRTLRND
                     PLQFEYLRNYYPVRREFSAYTLHTKGLNHELRLKLNQLGFDGG"
     gene            complement(6976..8088)
                     /locus_tag="PKZ74_06550"
     CDS             complement(6976..8088)
                     /locus_tag="PKZ74_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92455.1"
                     /translation="MKKLAITVAILTASFTSGGQQGNPELQKQVDELKARVKQLEKEN
                     IVFIAGNKTEDSLDYALCRAEIYKIFLHLQKLDGDYQHTSDKLAVTGLFSKLIQAGNP
                     TSDILGFRFTDVIMTSVDKHFIQYLKDEKDKKRLINVIGKIMDNPVVSNLANTNPVTS
                     VIASVIHIVSDFSTTSVELKKNGRKVTGAGVNEQDIFDSPSIKNFRASLQIYIDFYDV
                     LNQITSEFVKSLDDLNKEYHTLNTRISLFKSDLYNTLNVNDENLFLRLSQALPDPSAD
                     RINNEIMITRAVVKDCYRFSDRFAVLREDADEYEIRYKTLLYSFLDDYIKTLGLINDF
                     PDSSIDKSRARELADEIKCYIKSRKLVDAVLLNVDE"
     gene            complement(8133..9290)
                     /locus_tag="PKZ74_06555"
     CDS             complement(8133..9290)
                     /locus_tag="PKZ74_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM92456.1"
                     /translation="MFRIIRILVLLLAFTNLMAQEKSSAIQNDDPFIKMVRATDALSA
                     FIFTSQLNMKQVFTFDTIRNTAEIRVKKSGAKILFLQIIPSESQKELLFFLDSAWVVD
                     HTIRNMALIGHTHDDLSHNFISTSYPFSLFTLDTMLAYVSPFWKITGENNDFYEVTMD
                     IANKSEDVSDVLVKFMIRKPDFLPYGLYQESVYLKVDKMFQEQILSDFRLLDTNEIEI
                     PGYYYMYERSGTHEHSMEMDSAIAGEPDTSSVYLSDLELFTLEGDKFEMYADGLVLLD
                     LWYVGCAPCMKSAPVLEKLYKQYGSMVHFLSINEVDRDTARIKLFKEKMGITVPALLG
                     GNNKIANRVSQYRGYPVFILFDASTGKVLWHIAGYSEELEKVIGEVIEKNL"
     gene            complement(9436..9945)
                     /locus_tag="PKZ74_06560"
     CDS             complement(9436..9945)
                     /locus_tag="PKZ74_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92457.1"
                     /translation="MKKLILTFIILNSVILSSQSQDKLKIGDVRNDKLVVSNVEGLKK
                     FLYNSIGNSGTLGKDLQYRLAPEGDRCVVFYPVSGNRQNVTSAGVLLIVKNGEAFIQE
                     SPEIIDSSGPGGGGSYEIQCVGTNCNQCVPAIKWIGNWLPYVICECKQEGDGTCNMIT
                     KLIVNVQFN"
     gene            complement(10291..>10481)
                     /locus_tag="PKZ74_06565"
     CDS             complement(10291..>10481)
                     /locus_tag="PKZ74_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92458.1"
                     /translation="FQGCNITEEFYDFVMTLQSETGFQTPLFSGPPANVKSNLSNGAV
                     GFFTAYSVAYSSKVFQLQ"
BASE COUNT         2787 a         2343 c         2306 g         3045 t
ORIGIN      
        1 tgagttaata gaaagtcagt cctatttttt tcaaaatatt ttttctcaaa tcattataat
       61 gtttaccctc ctgttctgag cttgcccctc ctgagttgaa ttatcatagg gcggattgga
      121 ttcgccaaac caattaatgg taatctgatc ggcatttgcc ccccgactga ttaaatacct
      181 cgcgacaaca tttgctctga atctcgataa cttcatattg tcagctgttc ctccaatgtt
      241 atccgagaac ccctcaattc ttatggattc tgaaggatta ctttccagat acttagcaat
      301 cttatccagt tcaggatagt atttctgatc gattatagcc tgcccgctct tgaataaaac
      361 ataatcaata tatgtaggtt gagcggatgg aattatattg ggtattggaa ttggaattgg
      421 atgtgtgcgt tcgacagtgt ctggtgaagt ctcttttggg attcttggtg ggtctgttac
      481 tatttttatt tggtcttttg tgatttctaa tactactctg cgattcatgg ctctgcctgg
      541 actggtacta tttggagcaa ttggcattct ttcaccgtgc cattttaggt tgatttgttt
      601 tggattgaca ccacgaatta taaagtaatt cttaacagaa tttgcccgat tatatgaaag
      661 tatcatgtta gctgaatcgg aaccaatgct atccgtgtgg ccggtaatta aaacaacaga
      721 tgaaggatct tttagtagac attcagcaaa atagtcaagg ataactattg aagtatcatt
      781 tattatgctc ttattgttat caaaatgtaa catggatggt gggggaggac aaggccttcc
      841 tgtagaatca ctttcttgtt cctttatctc aattgtctta tttatttgcg aatctgtcct
      901 gacgctttta tcaataatta ttttcgaatt cataatgtta atgctatttt cctggtggac
      961 aggatcaaag ggtgtttttg aaatctccgg acagtaaact ttacattttt cacaatccac
     1021 agaatcatgc gcacatccgc aacaagtaca gcaaacaatg atctttacat cattacctcc
     1081 tttagccttt tttacttttg gagttttatt tgggtcatta tgtggcaccg ggatcttcac
     1141 ccccacgtat cctttcattc cgtatggatc ttcaaaaacg gtcgaaaaca ggttgctgac
     1201 gccgaagtaa acgatacccg ccctgactcc caatcctgct tcaaaatgtc cgtactggtc
     1261 aacctggatg ggtagggcaa aaccatacca cttttgctca taccgtggag tgaccgagac
     1321 cgtggtcagg taatgattgt gcgcaggctt gctatatccc tgatttaaag cggtataggt
     1381 tgtgagattg acatatacgc cttcgatcag gtaaacgtcg gcctggatac tgaaagcggt
     1441 cggcagactg actttgaact cttctttgtt atcttttgcc ctgacaactc ccagatcatg
     1501 tccggatgag taccgccggt acatcgtgtc ggcaaacgga acgtacttcc tgaaggtgaa
     1561 atccaccgag ccggcattga gccaggaggt attcaccggt acgctgttat cgcttgaatt
     1621 gtagcggttt acgtaatcgg gtgtgaatga ggtcaccagg tcggaactgt ggtaatcttt
     1681 cacgtatttc aaccggccca ggtcaaccac cgaaatgcca acctttatga aatacttgtt
     1741 ccggtcagcc cgcggcttgc cggtcttcgg attgttgtgc tccatccagc gcggacggta
     1801 ctcatacacc gcgccgaaat ccattccgaa agagggtctg gcagttaact ggaaattcat
     1861 gtcgaatgca taactgccat ccggcctcca gtatccccag ttgtcggata atccgccctg
     1921 aacatagggt gaattccagg aaatgggttt ggcgccggaa ggttcagcac catcgtagta
     1981 ataataaagc tgatcggttt gcaggtatga actggcaatc ccctggagca gtttgagcga
     2041 aacaccggct ttgatgaaat gctcccgctg gtcgtagaat acccgggcgt aggtgagtcc
     2101 gtaatccccg aaaatgtgct gcaccgccct catgttatca tcataatacc aacggttcca
     2161 gtaaaggctc tctttttccg aaaaattgct gtagatcatc cggaaaaggg gctcatccat
     2221 gccatcgaca ttggtgatgc tgcggactcg ggaggtgagg gcaaaggcgt gcctgctgcc
     2281 catggagatc atgaggctgg ggccaatggc cgattgcgac atgaacatgc tcttgtcttt
     2341 gccgttggct ttgtcaagat aagtattttc atcccaccag tcagcgttgg taagtatgcc
     2401 tgtaggatca agtaaccacc tcgatttgaa gcggataccg gtgctgacga tgtcgctgct
     2461 gaaacctccg gcgttgaaat cggatttgaa acggctgtcg gcaatggagg cgggctgcaa
     2521 aaatgcctgg ttaacaccca tataattgtc gttcagcaca ggcagaaagt cctgcccggc
     2581 cgacaaaccc ggaatagcca gcatggccgc aagcaataac agatgtagga tagttttttt
     2641 caatttgaaa ctgccattac cgatttttaa tacgcattat aaatcaatga ctgctctagt
     2701 gttatactca tttactgcag gccggtttca ggaaccggcc tctttttttt catttgattc
     2761 gatgcaggtt gaaaatacat taactttacc cgctacaaaa aaaaccgtta cgctattgat
     2821 cttcaattgg atgaataata tttttttgag gataattact tccgtcattt ttgtctcttc
     2881 tgttggatta accgaaccgg tatctgccca ggatcagccg ggcctgatga gaaccgccga
     2941 ggattatagt tcactgataa cccttaacta ccaggatacc ctgcttttaa acgtgaatga
     3001 agaatttgat cttccggtga tcatcaagaa acaaagtaaa atatctgcca tctctctggg
     3061 ctttttttac gatccggttt acctggaaat tgacagtatg attttggcca agggtacatc
     3121 cggattcttt ttcagcgtca acgacagcgc attccaaatg gcctggtcca gcatccagcc
     3181 ccttgaattg gaggatgggg atacgctctt agtattgaac atgaagacgt tagatatatc
     3241 tgatttgact aacactttaa ggataagctt gaatgaaacc agtgaactgg cggatacggc
     3301 agccaatccg attgaggatg ttgttctgga gattcccgag attggttact taattcctga
     3361 tcatcacgac tctataggtg aatcaacaat atgcatttat ccgaatccat tccgggatca
     3421 tgcaacagta ttttttactt tgaaagtagg ttctgaagtg aaaatcacag tgaataactc
     3481 atcaggaatt gagattttca ctttaactga ccggccatat gaacccggaa atcatgagat
     3541 tagattagat aaactgagga tgcccagagg gatctattta ctgaaatttg aaattagaaa
     3601 ttcggataat tcagggcaga aggtgtttaa aattatttca gccttttaac cttaaaagat
     3661 ctaaataggt tcctgaagaa aatcaagtac catagctttg atattctgag cattaaaccc
     3721 aaacttttca tccagtacct ggaatggcgc cgaatacccg aaatgttcca gtccgaatac
     3781 ttttccctta caacccgcca ggttctttaa cgtaaccggt aaaccggctg ttaacccgaa
     3841 gacaggggta ttatccggaa tgattgactt gcggtaatgt tcatcctgaa gttgaaaaag
     3901 tccctctgat ggcgctgaca ccacacgggc ctgaatattg tgatcattct tcaggagaaa
     3961 agcacactca atcagagttg aaacttccga accgttggcc agtagaatca catccggttc
     4021 attatcaggt tcataaacag tataagcgcc tttgcgggta tgaacggcct ccttgagcct
     4081 gttgcctccc gccggagccg gtatgtcttt gatattctgg cgtgacagga tcagggctgt
     4141 cggtgtttcg gtattttcca gggccagttt ccaggaaatg atggtttctg cgctgtcggc
     4201 cggccgtaat accaataggc tgttgtgacc ccggtggttc ttaagttgtt cgagcaaacg
     4261 cagttgcatt tcatgttcca ccggttgatg cgtagggcca tcttctccta cacgaaaaga
     4321 gtcgtgcgta aaaacatact ttaccggcaa ccgcatcagg gcagcgagcc ggatagccgg
     4381 tttcatataa tcggaaaata cgaagaaggt gccgcagaca acaaaaagac cgccatgaag
     4441 ggcgataccg ttggcaattg cggccatggt cagctctgat acacccgcat gcagaaatcc
     4501 gccattaaga tcgccgggtt gaaagggcct ggaatgtttc aggaaccctt cggttttatc
     4561 gctgttggat aaatcggccg acataacgat catatttcca atactttttg aaaaatatga
     4621 caacacgtcg cccgaagtat ttctggttgg ggcatccggc tttatgggaa tagcctccgt
     4681 atctatatcc ggtacttccc ccgaagtgta attattccac aataatgcct ggtctggatt
     4741 atcatctttc caggctgtca tttgatcatc tctgtctttg gtgtaaatac gtttcttgct
     4801 taatattaat ctataatact cttttacttc atctgatact tcaaatggat tatcaggatc
     4861 tccgcccagc ttccggattg tatcagacaa ggaaacaccc gctttggata aaggttgccc
     4921 gtgcgtggat gtgaggccct ccattctatt accttgagaa tcaacagccc cgaggcccat
     4981 gatggttttg ccgatgatca ggctgggacg ctgttcttct gccagggcat ttgaaatagc
     5041 ctccctgatc tccattgcat tgttaccgtt gatggtctga acataccaat tccaggagcg
     5101 gtatttcata gcggtatctt cgctggtaac ttcgccaatt tttgtagata actgtacatt
     5161 atttgcatca taaaacatga ccaggttgtt cagcccgaga taaccggcta tgcgaccggc
     5221 tccctgggag atctcttcct ggatacctcc gtcggagatg aaggcaaatg tcttgtgagc
     5281 catccactcg ccaaaccggg cagccaggaa ccgttccgct atggccgagc cgaccgccat
     5341 cgcatggcct tgtccgagcg ggccggaagt gttctcgatg ccgtgcagca catcgagttc
     5401 cggatgtccc ggcgtgatgc ttttccattg acggaaattt ttcaggtctt cagtagaata
     5461 atagccgcac aggtggagca cagaataaag catgggcgac atatgtcccg gatcaaggaa
     5521 gaagcggtcg cgcaatggcc aggcaggatc gtcgggattg aaattcaaaa actcggaata
     5581 gagaatattg atgaaatcgg cgccacccat tgcgcccccg gggtgcccgg aattggcttt
     5641 ttccaccatg gcagccgaca gaatgcggat ggtatccgcg gcgaggtcaa cgatcggttt
     5701 gctcatcgca aagttatatt ttggttaaga tttgcgataa aaatagatca gaattcggga
     5761 gcggttcaag tatcgttgat aaattctgaa aaaaacatgc ggctatccac catcaaatcc
     5821 cagctggttg agcttcagcc taagttcatg atttaagcct ttggtgtgca atgtataagc
     5881 gctgaattca cgccgcacag ggtagtagtt tcgcagatat tcgaactgca acggatcatt
     5941 tctcaatgtc ctgtcatctt ttaaaacatc ataagtgccc agaatagctt catcgatgat
     6001 ccgctcatct gacctgccgg ccgacgtaat ctcaatgaca ggtttttccg gtccgggaat
     6061 attttccgga taccattcat cgataccaaa accgaaatac cgggaggctg ccctgaccac
     6121 ggctgctgtg ccgttggctt ttccttcgac cgaataaccg gcgatgtgag gcgttgcaat
     6181 atcggctatt gcaagcaatt ccctgtcgat atgcggttca tcctcccaga catcggcaac
     6241 gaaaccccgg atcatgccct tttgatgcgc cacttttaat gcggaaccat ctacaaccgc
     6301 tccgcgagaa gtgttgataa tcaatggctt cttagccagg ctattgaaaa aggcatcgtt
     6361 tgccagatga aaagtccggt catcacctcc aaggttaagc ggaacatgga aagtaattaa
     6421 atcacactcc tctttgatct tttgcagcga aacaaaaccc tgttgccctt cgtttctggc
     6481 cctgggcgga tcattgagca atgggctcat tcccaatatt tccgccaata atgcgatttt
     6541 ctttccgaca ttgcctgcgc cgataatacc aattgttttt cctgcaagac tgatatgata
     6601 ttttgttgag agccttgaaa aggcagaagc catatactgc atgaccgatc cggaattaca
     6661 gccgggagcg tggtaccatt caatgcttcg gttattacag tattgaatat caatatgatc
     6721 ggttccaatg gtggcggatg cgatgaattt aaccggtata ccgtcaagca attcagcatt
     6781 gcatcgggtc cgggtcctta tgatcaggcc gtcggctttg gatgcaatat ctttgctgat
     6841 tacgccgccc ggagcataag tcactcttgc gaatggttcc agaacacctt taatgaacgg
     6901 aatatggtca tctattataa taaccggatg tttcaaatat aaaagctatt tatcttttga
     6961 caaaattaaa acaattcatt catccacatt taagagaaca gcatctacta atttccggct
     7021 ttttatatag cattttatct catctgcaag ttcccgggcc ctggatttat caatactgga
     7081 atcgggaaaa tcgttaatta aaccaagagt tttaatatag tcatccagaa aactgtataa
     7141 caaagtctta tatcggatct cgtattcatc ggcatcctct cttaatacgg cgaacctgtc
     7201 tgaaaaccgg taacaatctt taacgactgc ccgggtaatc attatttcat tatttatccg
     7261 gtcggccgag ggatcgggaa gggcttgtga taaccggaga aacaggttct cgtcattaac
     7321 atttagcgtg ttatacaaat cactcttgaa taacgagatc cgggtattca acgtatggta
     7381 ttctttatta agatcatcca ggctttttac gaactctgat gtgatttggt tcaatacgtc
     7441 ataaaagtcg atatatatct gcaatgaggc ccgaaaattt ttgatggacg ggctgtcaaa
     7501 gatatcctgt tcgttgactc cggctccggt gacttttctg ccgttttttt tcaattccac
     7561 tgaagttgtt gaaaaatctg aaacaatgtg gattaccgat gctatgaccg atgtcaccgg
     7621 gtttgtatta gccaggtttg aaaccaccgg attgtccatt attttcccga tcacgttgat
     7681 cagtcttttc ttatctttct catctttaag atattgaata aagtgtttat cgaccgaagt
     7741 cataataaca tccgtaaaac gaaagcccag gatgtcgctg gtgggattgc cagcctgtat
     7801 caatttggaa aataaacctg tcaccgctaa tttatctgat gtatgctgat aatcgccatc
     7861 gagtttttgc aggtgcagga atattttata gatttctgca cggcacagtg cataatccag
     7921 gctgtcttcc gttttatttc cggcaataaa aacaatattt tccttttcaa gctgtttgac
     7981 cctggctttt aattcatcta cctgtttttg caactccgga ttaccttgct ggcctccgga
     8041 agtaaatgag gcagtgagaa ttgctacagt tatggccaat ttcttcatac acaatattct
     8101 tccccattgc catgttgaaa tagtgaaacg gtttaaaggt tcttttcgat cacctctccg
     8161 ataacttttt ccagttcttc ggaatatcct gcaatatgcc acaatacctt cccggtagaa
     8221 gcatcgaata gaataaatac cggatatccc ctgtattgtg aaacccgatt agcgatttta
     8281 ttattacctc ccagcaaagc aggaacggta attcccattt tttccttaaa gagtttgata
     8341 cgggcggtat cccggtcaac ctcgttgatg ctgagaaagt gaaccatact gccgtattgc
     8401 ttgtaaagtt tctccagcac cggtgccgac ttcatgcagg gagcgcaacc gacataccag
     8461 aggtcaagaa gaaccagccc gtcagcatac atctcaaatt tatcgccttc cagagtgaaa
     8521 agttccaggt cagacagata aacactactt gtatcgggtt cacccgcaat agccgaatcc
     8581 atctccatgc tatgttcgtg cgtgccggac ctttcataca tgtaataata tccagggatt
     8641 tctatctcat tagtatccag cagtctaaag tcagatagta tttgttcctg gaacatttta
     8701 tcgactttca gatatacgga ttcctggtac aatccatagg gcaggaaatc gggttttctt
     8761 atcatgaatt taaccaggac atcggagacg tcctcggatt tgttggcaat atccatggta
     8821 acttcataga aatcattgtt ttcgccggta atcttccaga aaggggagac gtaagcaagc
     8881 atggtgtcca gggtaaaaag ggaaaacggg taggatgttg agatgaaatt gtgggacaga
     8941 tcatcatgtg tatgtccgat tagggccata ttcctgattg tgtgatcgac cacccaggct
     9001 gagtcgagga agaaaaggag ctctttctga ctttcagagg gaataatttg cagaaacagg
     9061 attttagcgc ccgatttttt aactcttatc tccgccgtat tgcgaatcgt gtcgaaagta
     9121 aagacctgtt tcatattgag ttgcgatgtg aatataaacg cacttaatgc gtcggttgcc
     9181 ctgaccattt tgatgaaagg atcatcattc tgaatggccg atgatttttc ctgtgccatt
     9241 aaattcgtga aagccaggag taaaacaagg atgcggataa ttctaaacat ggttttaaag
     9301 ggttaatggt gagacaatta aatttatatt taacttattt aaatttattt cttcgattcg
     9361 gtttaaagac ttccccggaa ggtgatattc ggaatagtgg aaattcgcca tccgggaaag
     9421 tcaaatcatc attctttagt tgaattgcac gttgacaatt aatttcgtaa tcatattaca
     9481 ggtaccgtcg ccttcctgct tgcattcaca gattacataa ggcagccaat ttcctatcca
     9541 tttgattgca ggtacgcatt gattgcagtt tgtaccaacg cactggattt cataacttcc
     9601 accgccgccg ggtcccgaag aatcaattat ttcagggctt tcctggataa atgcttcgcc
     9661 attcttcacg atcagcaaaa ccccggctga ggtcacattc tgcctgtttc cggaaacagg
     9721 gtagaaaacc acacagcggt cgccttcagg agcaagcctg tactggaggt cttttcccag
     9781 agttccgcta ttcccgatac tgttgtacag gaactttttc aatccttcca cattggaaac
     9841 cacgagctta tcgtttctta catccccgat cttcagcttg tcctgtgatt gagacgaaag
     9901 gattactgaa ttcagaataa tgaatgttaa aataagtttt ttcataaccg atgaattaaa
     9961 gtattgccta ctctgacagc ccttttcggc aaccgggcac tattccactt tcgcagtcga
    10021 actacctcag ccctgcaaat ttctacgcac tgcattaaag gaagaaccgg cgccgccagc
    10081 agcgatatta ttttcataat aacaaggctg tggcagaggt tttcagtaat gaattctggc
    10141 caaaaaggat taggttaagg gaagatgatt ccttctttcc tgaatggagt tttaccaggg
    10201 gggccggccc ggatgcatca ggtaatgatc ttccttacct gttaatccgg attttgggta
    10261 aaaaataccc ggtcgaatga aaatattttt ttattgcagc tggaaaacct tgctggagta
    10321 agcaacgctg taggccgtaa aaaagccgac tgcgccattt gagaggttac ttttaacgtt
    10381 tgccggaggg ccgctgaaca acggggtctg aaatccagtt tctgattgca gggtcatgac
    10441 aaaatcgtag aactcctctg ttatattgca tccctggaag g
//
LOCUS       DAOPNH010000097        10398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6915_length_10398_cov_6.885526, whole genome shotgun sequence.
ACCESSION   DAOPNH010000097 DAOPNH010000000
VERSION     DAOPNH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_10398_cov_6.885526"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKZ74_06570"
     CDS             <1..1049
                     /locus_tag="PKZ74_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPM92459.1"
                     /translation="TDHECFELDEALEKASMGMHILIREGSAARNFDDLSPLLESYPD
                     QVMFCSDDRHPDDLLKRHINDIARRAINKGYDPVAVIRACTLNPKKHYNLDTGLLQPG
                     DPADLIVIDDLSSFHVLKTYINGQMVASEGNSCIPRIEEIPVNRFDARPVSHSDLKVA
                     KKGNRIRIIEALEGQLITHELDGSPMVSGDEIISDPGQDILKIMVMNRYNPSSPAIGF
                     IRNFGLKRGAIASTVAHDSHNIIAVGVNDDDIMLAVNALVDTRGGVCVADQGHISLLP
                     LPVAGLMSVEDGFTVAEKYESLDRMAHELGSRLRAPFMTLSFMALLVIPELKLSDKGL
                     FDGKSFSFASLFLD"
     gene            1126..1428
                     /locus_tag="PKZ74_06575"
     CDS             1126..1428
                     /locus_tag="PKZ74_06575"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM92460.1"
                     /translation="MAKIQIQIEKLEAAASKLRAIAHPMRIAIIDLLHDGNQMSVTEI
                     YKKLKIEQAAASHHLNILKNKGVLGSRRDGKKIYYTIKNQVLTEIIDCINRCNNEV"
     gene            complement(1430..3130)
                     /locus_tag="PKZ74_06580"
     CDS             complement(1430..3130)
                     /locus_tag="PKZ74_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM92461.1"
                     /translation="MLLLLLPVIFTHCAKQGTPTGGPKDVRPPEVLEEIPPARTVNFT
                     AKKFTVVFDEFIQLKDPGKEIFISPPMKTKPEYKIHGKKLSVEFQEELKPDATYTINF
                     GNAIVDYTESNPLINYEYVFSTGDHLDSLSIPGLIVGAFDLKPVEGIVAMVYQDDNDS
                     IPFDSLPMRVPPKSASKTTKEGLFSINNLPAGAYRLFALEDLNNNYYYDLPNERIAFI
                     DSLVTLFPPAHTVVMPDTAIVDTSGVVAPVITAVMPDYTLYLFQESDSSQKFLGKKLI
                     GQSLLQYYYRMPVDSFHISPVGFEPVNPDWYMGDISRNRDTLGFWLRPGLPDTIRIRM
                     AAGDSLVDTSRYILTAAGKERPGRRKETASRAMSINTSSYAGFLDLNKKFYLKFGVPV
                     MDYDSSMLLLQTPADTLIPSIYFSDSLSRQLQVDYNWQPGEAYALVLDDSAFTDLSGA
                     HNDSLIFRFRVRTLEDYGILILNLTVPDDTGQYIIQLLNEKEIPVRETVINSSGQVKF
                     DYLAPGKYKLKATYDRNINGRWDPGKYIINSLPEKVEYYPTTLEIRANWEMQEEWKLD
                     "
     gene            complement(3197..3802)
                     /gene="nadD"
                     /locus_tag="PKZ74_06585"
     CDS             complement(3197..3802)
                     /gene="nadD"
                     /locus_tag="PKZ74_06585"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPM92462.1"
                     /translation="MSEPVKTKKVGLFFGSFNPIHTGHMIIAGYMLHFTDLDDVWFII
                     SPHNPLKEKSTLLADHHRYMMVQTAIEDNPRLRASNIEFKLPQPSYTIDTLIRLEEKH
                     SDHQFVLIAGTDIFPTFHKWKNYEVLLENYRFYIYNRPGYDAGAYSSHPNVVTFNAPL
                     MEISSSFIRQAILEGRDIRYFVPEKVYTYIREMHFYEKNNR"
     gene            complement(3799..4365)
                     /gene="gmk"
                     /locus_tag="PKZ74_06590"
     CDS             complement(3799..4365)
                     /gene="gmk"
                     /locus_tag="PKZ74_06590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPM92463.1"
                     /translation="MSGKLIIVSAPSGAGKTSVVKRLMQAELNLEFSVSATSRAPRPG
                     EIHGKDYFFIPVEEFRAKIDRDELLEWQEVYPNQYYGTLKSEVERIWNGGHHVLFDVD
                     VMGGMNLKKMYPEISLSLFIMPPSIKVLEQRLRHRSTETEESLRKRLDKAGKEIGFAG
                     HFDMTIVNDILEQAVAKAIETVSKFVRI"
     gene            complement(4367..5242)
                     /locus_tag="PKZ74_06595"
     CDS             complement(4367..5242)
                     /locus_tag="PKZ74_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPM92464.1"
                     /translation="MIRSMTGFGKCQADLPGKVITVEIRALNSKQLDLNFRAPAVYRE
                     KEMIARTLITQKLERGKIDATITAESKNEQLAVTVNKAIALHYLEQLKDLSSALPQTQ
                     NIDYLSVLVRMPDVLKTEKEELSEHEWDAIRHAIETALEEADTFRKNEGKILENDFIM
                     RINRITALLESIEPLEKARVATIRARLTSRLDELNIEKSFDENRLEQELIYYFEKLDI
                     TEEKLRLRKHCDFFILTLDEMESPGKKLSFITQEIGREINTLGSKANDASIQVIVVRM
                     KDELEKIKEQLANIL"
     gene            5420..6490
                     /locus_tag="PKZ74_06600"
     CDS             5420..6490
                     /locus_tag="PKZ74_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HPM92465.1"
                     /translation="MTDTAPYRLFDVPAGYFDAMIRDIGSARKYVYFEIYKYYNDAIG
                     GRFREALTQAAARGVEVKLLIDSWGANVSDDYFRELTDLRGEVRFFRKIRIVIDFFTK
                     NHRRNHRKLLIIDDHISWIGSGNVTEYSLEWRELMLRMEGDIATSFKKVFNLDFKIYN
                     RYFFEKNSYIRLIRHGDYLIVRDVPSITKKRINKKFIQMIRHADTSVEIETPYFLPGY
                     FLRKALLDACKRGVEVTVVVPRHSDVMLIDILRNRYIGPLHQGGLKFRYYMPGNLHAK
                     AILVDRQLFAIGSPNFDYRSFRYMHEIILIGREHSIIDQLGSHIDETMNHTEPFDFEK
                     WLDRPKLQQFIEWLLLPFRHFF"
     gene            6572..7780
                     /gene="rocD"
                     /locus_tag="PKZ74_06605"
     CDS             6572..7780
                     /gene="rocD"
                     /locus_tag="PKZ74_06605"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPM92466.1"
                     /translation="MTSKLAMELEDKYGAHNYHPLPVVLSKGEGVFVWDVEGKKYFDF
                     LSAYSAVNQGHCHPRIVQTMIDQAKTLTLTSRAFYNDALGVYEKYVTEYFGYDKVLPM
                     NTGAEGVETALKLCRKWAYMKKGIPEHEAKIIICEGNFHGRTITVISMSTDPDARGGF
                     GPYTPGFITIPYNDLEALAKALEQPNVAGFLVEPIQGEAGVYVPDEGYISKAAALCKA
                     KNVLFIADEVQTGIARTGKLLACDHENVRPDMVILGKALSGGVYPVSAVLADDEIMMC
                     IKPGQHGSTFGGNPVAARVAITALDVIRDEKLADNSARLGAIFREELKNINSDMIELV
                     RGKGLLNAIVVRPKNGKTAWDVCVKMAENGLLAKPTHEHIIRFAPPLVITEEQVHEAV
                     DIIRKSILSF"
     gene            complement(8005..>10398)
                     /locus_tag="PKZ74_06610"
     CDS             complement(8005..>10398)
                     /locus_tag="PKZ74_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM92467.1"
                     /translation="VVGSTTGAATDRNGNYTLIIPAGSELTVDFSYIGYKSRRFQVTL
                     QPNEKRELNVVLTIEFRELKDVEIKDDQVRSYNYVRLNPKEARLIPTMSGGIETMLKT
                     LPGVSSTNELSSQYSVRGGNFDENLVYVNGIEIYRPFLIRSGQQEGLSFVNSTLVSGI
                     VFSAGGWEAKYGDKMSSVLDITYRKPEKFAGSAQVSLLGAEGHIEGVNKSRRLTFLTG
                     ARYKTNQYVFRGLETKGSYKPNFIDVQGVVTYDMTETLQLSVLGYYSRNSYLLVPESR
                     QTNFGTIQEAYRLTIYFDGQEVDLYRMYLGAASVDYRPSSRLMMKFIVSGFDTRESET
                     FDIRGQYWIGKLENAFGDEEFGNVTEVQGVGTFMDHARNYLDAGVISAEYKGNYLWGK
                     NIAGWGIKYQHEEVSDKLAEWELIDSAGYSLPNPPVIIGGITDPKALLVIDRSVKTDI
                     TLSTNRLMGYAQNTWDLLGDGRDLNVTAGVRFHYWDYNGQFLFSPRASVAYKPQWKRD
                     VVFRFSTGYYYQPPFYKELRNLEGELNPDIRAQKSIHFVAGVDINFLAWKRPFKFTGE
                     AYYKYLDDLIPYVVDNVRIRYLSENSARGYATGVDFKINGEFIEGTESWMSLSFLKTE
                     QDIIGDFYYEYYNAAGEKIIRGYTADQVVADSARFEPGYIPRPSDQRVSFAIFFQDYL
                     PIIPSMKAHLTLIFGTGLPFGPPDSPLYKHTRRYPPYRRVDIGFSKLLVGEKTKFKEN
                     NPLRYIDKAWLSFEVFNLFQISNTVSYIWVKDINNRQYAVPNYLTPRLLNLKLIVEL"
BASE COUNT         2477 a         2610 c         2657 g         2654 t
ORIGIN      
        1 ctaccgacca tgagtgtttt gagcttgatg aagcgctcga aaaagcgtcg atggggatgc
       61 atatccttat ccgcgaagga agcgccgcgc ggaatttcga cgatttgtcg ccgttgctgg
      121 agagttaccc ggaccaggtg atgttctgtt ccgatgaccg ccatcccgat gatttgctga
      181 agcgccacat caatgatatt gcacgccgcg cgataaacaa agggtacgat ccggttgcag
      241 ttatccgtgc ctgcaccctg aatcctaaaa agcattacaa cctggatacc ggactgcttc
      301 aacccggcga tccggctgat ttaattgtga tcgatgacct ttcatctttc catgtgctga
      361 aaacctatat caacggccag atggttgctt cagaaggcaa ttcctgcatt ccccgtatcg
      421 aagaaattcc cgtgaaccgg ttcgatgcca ggcctgtgag tcatagcgat cttaaggttg
      481 cgaaaaaagg caaccgcatc cgtatcatcg aggcgctgga gggtcagctt attacgcatg
      541 agcttgacgg ttcgccaatg gtatccggcg atgagatcat cagcgatccc ggtcaggata
      601 tactgaagat catggtaatg aaccgttaca acccttcatc gccggcgatt ggatttatcc
      661 ggaatttcgg gctgaagaga ggagccattg cttcaacggt tgcccacgac agtcataaca
      721 ttatcgccgt aggtgtgaat gatgacgata tcatgctggc cgtcaatgca ctggttgata
      781 cacgaggcgg tgtttgtgtt gccgatcagg gtcatatttc gcttcttccg ttgccggttg
      841 ccggactgat gtcggtcgaa gacggattta ccgtagcgga aaagtacgaa tcgctcgacc
      901 ggatggccca tgaactgggc agtcggcttc gcgctccgtt catgactctc tcctttatgg
      961 ctttacttgt cataccggag ctaaaactga gcgataaggg tttgttcgat ggtaaaagct
     1021 tttcttttgc ttctcttttc ttagattaaa tataaattag caaaaaagat atatacatat
     1081 gaattgagtt ctgatgtatt ttctatttta gcaaaaaatt tagtcatggc aaaaattcag
     1141 attcaaattg agaaattaga ggcagccgcc agcaagctca gagcgattgc ccatccgatg
     1201 cgtattgcga ttatcgacct tcttcatgat ggaaaccaaa tgagtgtgac cgaaatttat
     1261 aaaaagctga agatcgagca ggccgctgcc tcacatcatc ttaacatatt gaaaaataag
     1321 ggtgtgttag gttcccgccg ggacggaaag aagatctact atacgatcaa aaaccaggta
     1381 cttactgaaa ttatcgattg tattaatcgt tgcaacaacg aggtataatt caatccagtt
     1441 tccattcttc ctgcatttcc caattagccc tgatttcaag ggttgttgga taatattcga
     1501 ccttttcagg aagtgaattg attatgtatt tgcccggatc ccagcggccg ttgatatttc
     1561 tgtcataggt cgcttttaat ttatactttc cgggcgccag gtaatcgaac tttacctgtc
     1621 cggatgaatt tattaccgtc tccctgaccg gtatctcttt ttcgttgagc aactgaatga
     1681 tatattgccc ggtatcatcc ggaacagtca gattcaggat aagaattccg taatcttcaa
     1741 gggtcctgac acgaaacctg aagattaaac tatcattatg cgctccgctc aggtcggtga
     1801 aagccgaatc atccagaacc agcgcatagg cttctcccgg ctgccagttg tagtcgacct
     1861 gcaattgccg gctgaggctg tcggaaaaat atatcgaagg tatgagggta tcggccgggg
     1921 tttggagcaa taacatggaa gaatcgtaat ccatgaccgg cacgccgaat ttcaggtaaa
     1981 atttcttgtt cagatccagg aacccggcat agctgctggt attgatgctc attgccctgg
     2041 aagccgtctc ttttcggcgt cccggccgct ccttcccggc agcggtcagg atataccggg
     2101 aggtatcgac aagagagtcg ccggcggcca tcctgatccg gatcgtatca ggtagcccgg
     2161 gacggagcca gaagcccagc gtgtcgcggt ttctggagat atcgcccatg taccagtcag
     2221 ggttcaccgg ctcaaatcca accggggaaa tgtggaacga atcgaccggc atccggtaat
     2281 aatactggag gaggctttga ccgatcagtt tcttgccgag gaacttttgg gaggaatcgg
     2341 attcctggaa caggtaaagc gtgtagtcgg gcatcacggc tgttatcacc ggggcgacaa
     2401 cgccggaagt gtccactatc gccgtatccg gcattacgac cgtatgagcc ggagggaaaa
     2461 gggtgaccag gctgtcgata aaagcgatcc gctcgttggg caggtcgtag taatagttgt
     2521 tgttcagatc ttccagtgca aagagcctgt atgcccctgc cggcaggtta ttgatactga
     2581 atagcccctc cttggtggtc tttgaggcgc ttttgggcgg aacgcgcatc gggagcgaat
     2641 caaaagggat gctgtcgtta tcatcctggt aaaccatcgc aacgatccct tccaccggtt
     2701 tgagatcgaa cgcgccgaca attagtccgg gaatggacag ggaatccagg tgatcgccgg
     2761 tagaaaatac gtattcgtaa ttgatcagcg gattgctctc cgtatagtcg acgatcgcat
     2821 tgccgaaatt aatggtgtaa gtggcatcgg gcttcagctc ctcctggaat tccaccgaga
     2881 gcttcttgcc gtgtatttta tattcgggtt ttgttttcat cgggggggag atgaagattt
     2941 ccttccccgg atctttgagc tggatgaatt cgtcaaaaac aacggtgaat ttttttgctg
     3001 tgaagttcac agtccgggca ggcggaattt cctccagcac ctccggcggc ctgacatctt
     3061 tgggccctcc cgtgggtgtt ccctgcttgg cgcaatgggt gaaaatgacc ggaagtaaga
     3121 gcaacagcaa aaaaatgatc gtattgcccc gttttttttt ggattttatt ttgaatgatg
     3181 aatggatcat gcctaatcaa cggttattct tctcataaaa gtgcatctcc cggatgtagg
     3241 tataaacttt ttccgggaca aagtaccgga tatcccttcc ttccaggatc gcctgccgga
     3301 taaagcttga agagatttcc atcagaggag cattgaaagt gactacattg ggatggctgg
     3361 agtaggcgcc ggcgtcatat cccggccggt tatagatgta gaaccggtaa ttttcaagca
     3421 gcacttcata attcttccat ttatgaaacg tgggaaagat atcggtgccg gcgatcagca
     3481 cgaactggtg gtcggaatgc ttctcctcga gccggataag ggtatcgatg gtgtacgacg
     3541 gctgcggaag tttgaattca atgttgctgg cccgcagtcg cggattgtct tcgatagctg
     3601 tctgtaccat catatagcgg tggtggtcgg ccagcagggt gctcttctct ttcagcgggt
     3661 tatggggcga aatgatgaac cacacatcat cgaggtcggt gaaatgcagc atatagccgg
     3721 caatgatcat atggccggtg tggatcgggt tgaatgatcc gaagaaaagg ccgactttct
     3781 tggtcttaac cggctcgctc atatgcgaac aaatttactg acagtttcaa ttgcctttgc
     3841 cacagcctgt tccaggatat cgttgacgat cgtcatatcg aaatggccgg caaaaccgat
     3901 ctctttaccg gctttgtcga gccgttttcg caggctctcc tccgtttcgg tcgaacggtg
     3961 cctgagccgt tgttccagga ccttgatgga gggcggcatg atgaacagcg acaacgatat
     4021 ctccgggtac atcttcttca ggttcatccc gcccatcaca tccacatcga aaaggacatg
     4081 atggccccca ttccagatgc gctccacctc cgatttcagc gttccgtaat actggttcgg
     4141 gtagacctcc tgccattcca gcagttcatc ccggtcgatt ttagcccgga attcttctac
     4201 agggataaaa aaatagtctt ttccatgaat ctcgccagga cggggcgcgc gactggtggc
     4261 cgatacggag aactccaggt tcagctccgc ctgcatcaac cgtttgacga ccgatgtttt
     4321 ccctgctccc gacggggcgc tgacgatgat gagtttaccg ctcatgtcag agaatattgg
     4381 ccagttgctc cttgatcttt tccagttcgt ctttcatccg gacgacgatg acctggattg
     4441 aggcgtcatt ggctttggag ccgagcgtgt tgatctcacg cccgatctcc tgggtgataa
     4501 aactgagttt tttccccggc gattccattt catccagcgt caggatgaag aaatcgcagt
     4561 gttttctcaa cctcagtttc tcttcggtga tatcgagctt ttcaaagtaa tagatcagtt
     4621 cctgttccag ccggttttcg tcgaacgatt tttcgatatt cagctcgtca agcctggagg
     4681 tgagccgggc acggatggtg gccacacgtg ctttttcaag cggttcgatg ctttcgagca
     4741 gcgccgtgat ccggttgatg cgcatgatga aatcgttctc cagaattttg ccttcgtttt
     4801 tacgaaacgt atcggcctct tccagcgccg tctcaatcgc atgccggatg gcatcccatt
     4861 cgtgttccga cagctcctct ttctctgttt tcagaacatc gggcatgcgt accagcaccg
     4921 ataaatagtc gatattctgg gtctgtggca gggccgatga gaggtctttc agttgttcca
     4981 ggtaatggag agcgatagct ttgttgaccg tcaccgccag ttgttcgttt tttgattctg
     5041 ccgtgatggt cgcatcgatc tttcctcgtt ccagtttctg ggtgatgagt gtgcgggcaa
     5101 tcatctcttt ttcgcggtag acggccggcg cccggaagtt gaggtcgagt tgtttgctgt
     5161 tcagtgcgcg aatctcaacg gtgatcacct tgccgggaag atcggcctgg catttaccga
     5221 aacctgtcat ggaacggatc atggggaact ttgttttgaa gagtcaaaga tagggaaaga
     5281 aatcggattt ggcgatttgg cgattggacg atttaatcgt cgactaacaa actaaatcga
     5341 aaatgcgcaa tgccgaatcg cgtaattcga ttgtccaact gtctcgctat tgtttcttat
     5401 ctttgcttaa aacatcacgg tgaccgatac agcgccatac cgcctctttg atgtgccggc
     5461 agggtatttc gatgccatga tccgcgacat cggctcggcc cggaaatatg tctacttcga
     5521 gatctataaa tactataacg acgccatcgg cggccggttt agagaagcgc ttacgcaggc
     5581 ggccgccagg ggcgtggagg tcaaactgct gatcgattcc tggggcgcaa acgtttcgga
     5641 tgattacttc cgggaactca ccgacctgcg gggagaggtg cgattcttcc gcaaaataag
     5701 gatcgtcatc gacttcttta ccaaaaacca ccgccgcaac catcgtaaac tgctgatcat
     5761 cgacgaccac atcagctgga tcggatcggg caacgtgacg gaatacagcc tcgaatggag
     5821 agagctgatg ctcaggatgg aaggcgatat cgccacatct tttaaaaaag tgtttaacct
     5881 cgacttcaaa atctataacc gctatttttt cgagaaaaac tcctacatcc gcctgatccg
     5941 ccacggcgat tacctgatcg tccgcgatgt cccttccatc accaagaaaa ggatcaataa
     6001 gaaatttatc cagatgatac gccatgccga taccagcgtt gaaatcgaaa ctccctattt
     6061 cctccccgga tatttcctgc gtaaagctct cctggatgcg tgcaaacgtg gcgttgaggt
     6121 cactgtcgtg gtgccgcgcc attccgacgt aatgcttatc gatatcctcc gtaaccgcta
     6181 tatcgggccg ctgcaccagg gggggctgaa attcaggtac tacatgcccg gtaacctcca
     6241 cgccaaggct atcctggtcg accgccagct cttcgccatc ggttcgccta atttcgacta
     6301 ccgctcgttc cgctatatgc acgagatcat cctcatcgga cgcgaacact caattatcga
     6361 tcagctcggc agccatatcg atgaaaccat gaaccatacc gagccgttcg acttcgaaaa
     6421 atggctcgac cggccgaaat tgcaacagtt tatcgaatgg ctcctgctgc ccttccgcca
     6481 ttttttctga ttcgcttttt ttcctccccc gaaaacccta actttgctgc aataattaaa
     6541 aattaagaat taaaaattaa gaatttctat gatgacttct aaattagcga tggagcttga
     6601 agacaagtac ggcgcgcata actatcatcc gctgccggta gtgctttcaa aaggtgaagg
     6661 tgtctttgtc tgggatgtgg aagggaaaaa atatttcgat ttcctgtcag cctattcggc
     6721 cgtcaaccag ggccactgcc acccgcggat cgtccagacg atgatcgatc aggctaaaac
     6781 gctgacgctg acttcccgtg ctttttataa cgatgcactg ggtgtatatg agaaatatgt
     6841 gaccgaatat ttcggctacg ataaggtttt accaatgaac acaggtgcgg aaggggtgga
     6901 gacggccctc aaactttgcc gcaaatgggc ttacatgaag aagggcatcc cggagcacga
     6961 ggcaaagatc atcatctgcg agggcaactt ccacggaagg accatcacgg tgatctccat
     7021 gtcgaccgat cccgatgccc gcggaggctt cggcccctac acaccgggat ttatcaccat
     7081 tccttacaat gatctcgaag ccctggccaa agcgctggaa cagccgaatg tagccggttt
     7141 cctggtagag ccgatacagg gcgaggcagg ggtttatgtg ccggatgaag ggtatatctc
     7201 caaagccgcc gctctttgca aagcaaagaa tgtcctgttc atcgccgatg aggtacagac
     7261 ggggatcgcc cggaccggca aactgctggc atgcgaccac gagaacgtcc gccccgatat
     7321 ggtgatcctg ggcaaagccc tctccggggg cgtttacccg gtttccgccg tactggccga
     7381 tgatgagatc atgatgtgca tcaaaccggg ccagcacgga tccacctttg gcggtaatcc
     7441 ggtggccgca cgcgttgcca ttacagccct ggatgtgatc cgggatgaaa aactggctga
     7501 taattctgca aggctggggg cgatcttccg cgaagagctg aaaaatatca attccgatat
     7561 gatcgagctg gtcaggggaa aaggactgct caacgccatc gtggtcaggc cgaaaaacgg
     7621 caaaaccgcc tgggatgtct gcgtgaaaat ggctgaaaac ggactgctgg ccaaaccgac
     7681 acacgaacac atcatccggt ttgcaccgcc actggttatt accgaagaac aggttcacga
     7741 agccgtcgat attatccgga aatcgatcct ttctttttag ttacagatat aaaaagcaaa
     7801 aggccacgcg ggcgcgtgac ctgtttgccg attattaacc aaaccaaaag agttgtttga
     7861 agagagccaa acaactgtac ctatgaaagt tctttttcag acgacaaaga tactgtacaa
     7921 tttttattta gacttaatca accctacaaa gatattaaca aaaatatccg gtgtcaagat
     7981 aatttttgtt aaaaatttct taaatcagag ttcaacgatt aatttcagat ttaagagcct
     8041 gggcgtcaga taattgggaa cggcatactg gcggttgttg atatccttga cccagatata
     8101 ggaaaccgta ttgctgatct ggaaaaggtt gaaaacttca aagctcagcc aggccttgtc
     8161 gatgtaacgc agaggattgt tctccttgaa ttttgttttt tctccgacca gcagtttgga
     8221 aaacccgata tccaccctcc ggtaaggggg atagcgacgg gtatgcttgt acagcgggct
     8281 gtcgggaggg ccgaagggga ggccggtacc gaatatcaat gtcagatgag ccttcatgga
     8341 tgggatgatc ggcaggtaat cctggaaaaa gatggcaaag ctgacccgct gatccgacgg
     8401 gcgtggtata tagcccggtt caaaccgggc gctgtcggct accacctggt cggccgtata
     8461 gccacgaatg atcttttctc cggcggcatt gtagtattca tagtaaaagt cgccgataat
     8521 atcctgttcg gtcttcagga acgacaggct catccacgac tcggtacctt cgatgaattc
     8581 cccgttgatc ttgaaatcga ccccggtggc ataacccctg gcgctgtttt cagacaggta
     8641 ccttatcctg acgttatcca ccacgtaggg gataaggtcg tcgagatatt tgtaatacgc
     8701 ttctccggtg aatttgaacg gacgtttcca ggccaggaaa ttaatatcga cgccggctac
     8761 gaagtggatc gatttctgcg cccggatatc gggattcagc tctccttcca ggttccgaag
     8821 ttctttgtag aatggcggct ggtaataata tccggttgaa aaacggaaga ccacgtcgcg
     8881 cttccattgc ggtttgtagg caacgctggc ccgcgggctg aaaaggaact ggccgttgta
     8941 atcccagtaa tggaacctga ccccagccgt tacgttaagg tcacgcccgt cgcccagcag
     9001 gtcccaggta ttctgcgcgt agcccatcag cctgttcgtt gaaagggtta tgtcggtctt
     9061 taccgaccgg tcgatgacca gaagtgcttt cggatcggtg atcccgccga taatgacggg
     9121 agggttcggc aatgaatacc cggccgaatc gatcagttcc cactccgcca gtttgtcgct
     9181 cacctcttcg tgctggtatt tgatgcccca tccggcgatg ttcttccccc ataggtagtt
     9241 gcctttgtat tccgcactta tcaccccggc atccaggtag ttccgggcat ggtccataaa
     9301 agtgccgact ccctgtacct ccgttacatt gccgaattcc tcatcgccga aggcattctc
     9361 cagtttgcca atccagtact gaccgcggat gtcgaaagtc tcgctttccc tcgtgtcgaa
     9421 tcccgaaacg atgaacttca tcattaaccg cgaactgggc cggtaatcga ccgatgccgc
     9481 tccgaggtac atcctgtaaa ggtccacttc ctgtccgtcg aagtagatcg taaggcgata
     9541 ggcctcctgg atcgtaccga aattggtctg gcggctttcg gggaccagaa ggtaggaatt
     9601 gcgtgaatag taaccaagca cggaaagctg cagggtttct gtcatgtcgt atgtcactac
     9661 gccctgcaca tcgataaagt tgggcttgta gctccctttc gtctccaggc cgcggaaaac
     9721 atactggttt gtcttatacc gggcgccagt cagaaaggtg agacggcggc ttttattgac
     9781 cccttcgata tgcccttcgg cgcccagcag gctgacctgg gccgatccgg cgaatttctc
     9841 cggtttgcgg taagtgatat ccagaaccga cgacatcttg tcgccatact tggcctccca
     9901 gccaccggcc gagaacacaa ttcccgagac cagcgtggag ttgacaaagc tgaggccttc
     9961 ctgttgcccc gaacggatca ggaaaggacg gtagatttcg ataccattga cgtacaccag
    10021 gttctcatcg aaattcccgc ccctgaccga atattgtgaa ctgagttcat tggtagagct
    10081 gacgcccggc agggttttca gcatggtctc gattcccccg ctcatggtcg ggatcagcct
    10141 ggcttccttt ggattgaggc ggacatagtt atagcttctc acctggtcat ccttaatctc
    10201 cacatccttc agctccctga actcgatggt cagaacaaca ttcagctcac gtttttcatt
    10261 gggctggagg gtcacctgga atctcctgga cttatacccg atataagaga agtcgaccgt
    10321 cagttcactc ccggcgggga tgatgagcgt atagttgcca ttcctgtcgg tggcggcccc
    10381 ggtggtgctt cctactac
//
LOCUS       DAOPNH010000098        10323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_6976_length_10323_cov_5.835314, whole genome shotgun sequence.
ACCESSION   DAOPNH010000098 DAOPNH010000000
VERSION     DAOPNH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6976_length_10323_cov_5.835314"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1022
                     /locus_tag="PKZ74_06615"
     CDS             168..1022
                     /locus_tag="PKZ74_06615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM92468.1"
                     /translation="MTEQFRPRGFTLLPPVVKNLLIINGIMFLAQFVAEKSFGIRLEE
                     HLGLRYPGASEFRPWQLISYMFLHGNFWHLFFNMFALWMFGNALENIWGTKRFLIYYF
                     ITGIGAALIHYLVFYFFDLSPVLSDINAIMAAPTYQAVSDFISGDPELFQIYLKEIYS
                     DPQKFFGAMEILRTSPDNKQAIYIVKNLLVDYREFYMNLPNVIGASGAVFGILLAFGM
                     LFPNTLIYIYFFFPLKAKYFVIAYGLIELFYGFTGSGSNVAHFAHLGGMIFGFFLILY
                     WKKKGRLY"
     gene            1030..1956
                     /locus_tag="PKZ74_06620"
     CDS             1030..1956
                     /locus_tag="PKZ74_06620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM92469.1"
                     /translation="MQYYRNPLEEAGRFFRSGNLLSRLIIINTAIWLLVGVLRVISFL
                     FKFPDKELMEFLIDVMALPADTGILITRIWTLFTYMFFHLDFFHLLFNMLWLFWFGKI
                     FLEFLKPVQLLFLYLTGGLSGGILYILFYNLFPVFQESLPVSVALGASASVMAIVTAI
                     SVYVPGYTVHLLFLGRIRIFYIALFLFVLDFFMIRSENAGGHIAHIGGAVFGLIYILS
                     LRKGMNFNGVFKMRWFKQFTSIFGRRKMKVVSGNYRQTTERPLTDDEFNRRRAERQRK
                     IDDILDKVARSGYDSLSKEEKDMLFNASNSGD"
     gene            1972..4011
                     /gene="uvrB"
                     /locus_tag="PKZ74_06625"
     CDS             1972..4011
                     /gene="uvrB"
                     /locus_tag="PKZ74_06625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPM92470.1"
                     /translation="MQFNLTSDFFPTGDQPEAIRQLKEGLDRGDPAQVLLGVTGSGKT
                     FTIANVIAQINRPTLVLSHNKTLAAQLYGEFRQFFPENAVEYFVSYYDYYQPEAYLPV
                     TNTYIEKDLSINEEIEKLRLSTSSSLLSGRRDVIVVSSVSCIFGIGNPDDFTRNVVKV
                     QIGDRISRNHLLYSLVDALYSRTETDFGRGRFRVRGDTVDVFPAYADIAYRIVFFGDE
                     IETIESFNPENGFKIEKQDNLTIFPANIFVTSQEKMKEALKEIEEDSWGQAAFFRDNG
                     KMFEAKRLEERVSYDVEMMRELGYCSGIENYSRYFDGRAPGTRPFCLIDFFPDDFLLV
                     IDESHVTIPQIRAMYGGDRSRKQTLVEYGFRLPSALDNRPLKFDEFESLVNQVIFVSA
                     TPADYELQRSGGVVVEQLIRPTGLLDPVIEVRPSQNQIDDLLGEIDITVKKGNRVLVT
                     TLTKRMAEELTKYLTRLGIKSRYIHSEVETLDRVEIMRELRLGKFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNIDSRVIMYADQITDSMRQTIDET
                     NRRREKQLAYNEAHNITPKAIIKSVERIMGQTAVADAKHKEPQPYFEKEEVEVAADPV
                     LKYLSDSQIQRSISDLRKEIEKAVKELDFIQAAKLRDEMYAMEEFLEDRKNDSKK"
     gene            4059..4832
                     /gene="cysQ"
                     /locus_tag="PKZ74_06630"
     CDS             4059..4832
                     /gene="cysQ"
                     /locus_tag="PKZ74_06630"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPM92471.1"
                     /translation="MAVSAAIEGGNEILQVYGQDFAVEYKNDNSPLTLADRRAHEAIM
                     RILEPSGLPVLSEEGKAIGFETRRNWGQFWLVDPLDGTKEFVKRNGEFTVNIALIKGN
                     EPLAGVIYIPVTGVLYFACQTTGARKTVLEPSGKQSFDQIIKDSTILPESFSPNKLTV
                     VCSRSHMSAETEKYIDQLRQTHHDVDFVSIGSSLKLCLVAEGKAHIYPRFGPTMEWDT
                     AAGHAIALFAGCTVRQADSGAPVVYNKPDLKNPWFIVSR"
     gene            complement(4894..6237)
                     /locus_tag="PKZ74_06635"
     CDS             complement(4894..6237)
                     /locus_tag="PKZ74_06635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM92472.1"
                     /translation="MKLKNVFSLITLAVILAVFATSCNIGTHPGYKKSKSGVYYKIHN
                     DNNDTTSVREGTIVTMDLKYGLKDSLMFDSKQSPNPIILQVAKSEYEGDFNECISLLN
                     QGDSATFILKAGPLFKTTFRQPELPEPLTDDSEIIFDVTIQKVQTQEQMEMETKLKNM
                     ELEKQEVIKLQEYIQKNNITAEPSSTGIYYIETDKGKGKSPAEETYLTAHYTVYLLDG
                     EQLFSTRERGEPVDFKYKSQYENEGFHEVLGMMREGGKAKAIIPSAMAFGEQGAGNIV
                     PPFSTLYYEIELIKVISKDEFNAKQQQKESKKKAEAEMKVKEEEANIQKYLKDNGLAP
                     TTTLPSGLVYIETKAGNGPKPETGKKVKVHYTGKLLNGNKFDSSVDRGEPFEFALGQR
                     QVIEGWDQGIALMSEGAKGLLVIPYRLGYKERGAGEDIPPYSTLVFEVELVEIEK"
     gene            complement(6254..6790)
                     /locus_tag="PKZ74_06640"
     CDS             complement(6254..6790)
                     /locus_tag="PKZ74_06640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM92473.1"
                     /translation="MHRRYLKVICILIGVTAILAISCNENPSTDRKEALPGLKEPLIH
                     ANKEAVREENQQIDDYIRRHQWAMETTPTGLRYMITHTGIGPKGETGRMVRLDFVVMF
                     LTGDTVYTARTEGPLEFVVGKGQVITGLEEAILLMRVGDRAKFIIPSHLAFGLIGDQK
                     KIRHKATLVYDLEFVSMK"
     gene            complement(6796..7821)
                     /locus_tag="PKZ74_06645"
     CDS             complement(6796..7821)
                     /locus_tag="PKZ74_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPM92474.1"
                     /translation="MKHPDIEIIKSIIKDSRSVVVTSHHNPDGDAIGSVIAMVKLLEK
                     RVPQVTGIVPNDFPDFLKWMPGADRIVIYNQLEPKAKELIYNADLIFCLDYNALHRTD
                     AMAPALKSARAVKVLIDHHLDPLSEDFDYIISRIDISSTSELLFNFIEQCGWSDLIGQ
                     EVATGLFVGIMTDTGSFSYSCNYPETFQISALLIKTGIDPEQIHRNVYDTYSESRLRL
                     LGYCLGEKMTILPQYKTAYIALTKAELDRFQYKTGDTEGVVNFALSIDEIRFAVLLTE
                     RTDRIRLSFRSKGNLSMNVFARKYFNGGGHRNAAGGDSFESMENTISHLKNALEQYKE
                     EIDRSGC"
     gene            7900..8319
                     /gene="ndk"
                     /locus_tag="PKZ74_06650"
     CDS             7900..8319
                     /gene="ndk"
                     /locus_tag="PKZ74_06650"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPM92475.1"
                     /translation="MNGNITLTMIKPEAVRNGHTGHILAKITEGGFGIVAMKYLKLTR
                     EQAEKFYEVHRERPFYKDLVEFMTSGPIVAAILKKDNAVEEYRSYIGSTNPKDAAPGT
                     VRALYGTNIQENAVHGSDSDENAAREASFFFSELERY"
     gene            complement(8313..8714)
                     /locus_tag="PKZ74_06655"
     CDS             complement(8313..8714)
                     /locus_tag="PKZ74_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM92476.1"
                     /translation="MIDRFRQLMADQGLSASEFADRIGVQRSSISHILSGRNKPSIDF
                     LEKIKTAFPEADLNWLVSGKKSATSGLSTSPAVSKTVPVSLPPTLFEAEENNEIQKKP
                     VHKDDEPVEQIIMIFRNGTFRVLKPESGKDQ"
     gene            complement(9125..>10323)
                     /locus_tag="PKZ74_06660"
     CDS             complement(9125..>10323)
                     /locus_tag="PKZ74_06660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HPM92477.1"
                     /translation="FDYGMGFNQIQMLNGDVFHEMGYRGQGKVIAVLDAGYFNANSVD
                     AFDSLWMNGQILGTKDFVRGGDIQFDEHQHGTMVLSCMGANFPGQLIGTAPMADYWLL
                     RSEDGGSEYLIEEYNWVSAAEFADSVGADIINSSLGYSQFNDPTQDHTYEDMDGNTTP
                     ATKGADMAAKKGILVVNSLGNEGNSSWYYLSAPSDGDSVMGIGAVDANGFYVGFSSHG
                     PSYDGRVKPDVATQGSMVYVVEPMGGGFTFGGGTSFSSPILAGMAACLWQANPAMTNM
                     QIADAIRKSASQYDNPDDMLGYGIPDFVLANNILTVINGPDKQHVSLNLYPNPVRTSF
                     TVSLDQVNANNADIIITDITGREIYTASLMGNEYTVNAKGLEAGVYFVSVSANGRTFT
                     GKFIKR"
BASE COUNT         2732 a         2290 c         2280 g         3021 t
ORIGIN      
        1 gtcagcctag agtcaaaaat gtgtcgaaaa ttatatcctg aagagatgat tcaccttata
       61 gatgagttat tttcatgtaa agtacctgat ttgaccccgg aaggcaaaaa ggtttatacc
      121 attttccctt tggaagagct aaaccggttg tttaaccttg aaaagctatg acagaacaat
      181 tcagacccag gggatttacc cttctcccac ctgttgttaa gaatttgttg attatcaatg
      241 ggataatgtt tcttgcgcaa tttgttgcag agaaatcttt cggcatcagg ctggaggagc
      301 atttggggct gagatacccc ggcgcttctg aattcaggcc ctggcaactg atctcctata
      361 tgttcctcca tggtaacttc tggcatctgt ttttcaatat gtttgccctc tggatgtttg
      421 gcaacgccct tgaaaatata tggggtacaa aaaggttcct gatctactat tttattacag
      481 gaatcggggc cgccctgatc cattatcttg tcttttattt ctttgacctg agcccggtat
      541 tatccgatat caatgcaatc atggcggcac ctacttatca ggcagtcagc gattttatat
      601 ccggtgatcc cgaactattc cagatatatc tcaaggaaat ctactcggat cctcagaagt
      661 tcttcggtgc gatggaaatc ctgcgaacca gcccggataa taagcaggca atttatatag
      721 tgaagaattt attggtggac tacagagagt tctacatgaa cctaccaaat gtcatcgggg
      781 catcaggcgc ggtattcgga atcttattgg cgtttggtat gttatttccc aataccctga
      841 tttatatcta tttctttttc cctctcaaag ccaaatattt cgtgattgct tacggattga
      901 tagaattgtt ttatggattt accggctcag gatcaaatgt tgcccatttt gcccacctcg
      961 gcggtatgat ttttggattc ttcctgatcc tttactggaa gaagaaaggt cgtttatatt
     1021 aatcccacta tgcagtatta caggaatccg cttgaggaag caggccggtt tttcagatca
     1081 ggaaatttat tatcccgact gatcatcatc aatacggcca tatggttatt ggtgggcgta
     1141 cttcgtgtca tttcctttct cttcaaattt cccgacaagg aattgatgga atttctgata
     1201 gatgtcatgg ctttgcctgc agataccggc attctgatca cccgaatctg gacgcttttc
     1261 acctatatgt tttttcacct cgatttcttt cacctgttat ttaatatgtt atggctattc
     1321 tggttcggaa aaatattcct tgaattcctg aagcccgtgc agttgctatt cctctatcta
     1381 acgggaggat tatccggtgg aatactttac atcctttttt ataacctgtt tccggtcttt
     1441 caggaatcat taccagtttc agtcgctttg ggcgcttcgg catccgtcat ggcgattgtc
     1501 accgcaattt ccgtctatgt tcccggttat acggttcatc ttttgttttt aggcaggata
     1561 cggatatttt atattgcctt gtttctgttt gtcctggatt ttttcatgat ccggtcggag
     1621 aatgccggag gccatattgc gcacattggt ggagcggttt ttgggttaat ttatatactt
     1681 tcgttaagaa aaggaatgaa tttcaatggg gtgtttaaaa tgaggtggtt caaacaattt
     1741 acttccattt ttgggagaag aaagatgaaa gttgtttcag ggaattaccg gcaaaccacc
     1801 gaaagaccac tgacagatga tgaattcaac cgcaggagag ccgaaaggca gagaaagatc
     1861 gatgatatcc ttgataaagt ggcccgttcg ggctatgata gcctgagcaa ggaggaaaaa
     1921 gatatgttgt ttaatgccag taactcagga gattaaacca aatcatacaa tatgcagttc
     1981 aatttgacct cggatttttt tccgaccgga gaccagccgg aagcgatcag gcaactgaag
     2041 gaaggtctgg atagaggcga tcctgctcag gtactgcttg gggtgaccgg ttcgggtaaa
     2101 accttcacca ttgccaatgt tattgctcaa ataaacaggc cgacacttgt actgagccat
     2161 aataagacac tcgcagcgca gctttacgga gaatttcgcc agttcttccc ggaaaatgcc
     2221 gttgaatact ttgtctctta ttatgattat taccagcccg aagcttactt gcctgttacc
     2281 aatacttaca ttgaaaaaga tctctccatc aatgaagaga tcgagaaact tcgtttaagc
     2341 acatcctcat cgcttctttc cggcagaaga gatgtaatcg ttgtttcatc cgtttcctgc
     2401 atcttcggta tcggaaatcc ggatgacttt acccggaatg tagtcaaggt acagattggt
     2461 gaccggatca gcaggaacca tcttttatat tccctggtgg atgccctgta ttcaagaacg
     2521 gagaccgatt ttggccgggg ccggttccgc gtgcgtggcg atacggttga tgttttcccg
     2581 gcctatgccg atattgccta tcgaatcgtg ttttttggcg atgaaattga aactattgaa
     2641 tcctttaacc cggaaaatgg gtttaaaatt gagaaacagg ataacctgac catttttcct
     2701 gccaatattt tcgtgacatc gcaggaaaag atgaaagaag ccctgaaaga gattgaggaa
     2761 gattcatggg gccaggctgc ttttttcagg gacaacggga aaatgttcga agctaaaaga
     2821 ctggaagaaa gagtatccta tgatgttgag atgatgcgcg aactggggta ttgctcaggg
     2881 attgaaaact attcccgcta ttttgacggc agggcaccag gtacccgccc attctgtctg
     2941 atcgactttt tccccgatga cttcctcctg gtcatcgatg agagccatgt gaccataccg
     3001 cagatcaggg cgatgtacgg aggtgacagg tcacgtaaac aaaccctggt tgaatatggc
     3061 ttcaggctcc cttctgccct tgacaaccgg ccgctgaaat tcgacgagtt tgaatccctt
     3121 gtcaaccagg ttatttttgt cagcgcaaca ccggcagatt atgaactcca gcgatccggc
     3181 ggtgttgtgg tggaacaact tatccgacct acaggcctgc tcgatccggt gatcgaagta
     3241 cgcccgagtc agaaccagat tgatgacctg ttgggagaga tcgatatcac ggtaaaaaaa
     3301 ggaaaccgtg tacttgtcac cactttgacc aagaggatgg cggaagagct tacaaaatac
     3361 ctgacccggc ttggaattaa aagccggtac atccattccg aagttgaaac gctcgacagg
     3421 gttgagatta tgcgggaact ccggctcggc aagttcgatg tgcttgttgg gattaactta
     3481 ttaagagagg ggctggatct ccccgaagta tcactggttg ccatcctgga tgccgataaa
     3541 gaaggttttc tccggtcggc ccgctcgcta acacaaacag ccggccgtgc cgctcgcaat
     3601 atcgacagcc gtgttatcat gtatgcagac cagatcacgg attccatgcg gcagacgatc
     3661 gatgaaacca accgcaggag ggaaaaacaa ctggcttata atgaggcgca taatattaca
     3721 ccaaaagcga tcattaaatc cgttgagaga atcatgggtc aaacagcggt tgccgatgca
     3781 aaacacaaag aaccgcagcc atattttgaa aaagaagagg tggaggttgc tgccgaccct
     3841 gtattgaagt acctgtcgga cagccagata cagagatcta tcagcgattt aaggaaggaa
     3901 atcgaaaaag cggtgaaaga actcgatttc atccaggcag ccaagcttcg cgatgaaatg
     3961 tacgcaatgg aggaattcct tgaagaccgt aagaatgatt caaaaaaata acagaccctc
     4021 ctcatatgac cagccctgag atattaggat tagtagagat ggctgtatca gctgctattg
     4081 aaggtggtaa cgagatattg caagtctatg gacaggattt tgctgtcgaa tataaaaatg
     4141 acaacagccc cttaacgctt gccgatcgca gggcacacga agctatcatg cggatacttg
     4201 agccttcagg ccttcctgtt cttagcgaag aaggcaaagc aattggcttc gaaacccgaa
     4261 gaaactgggg ccagttctgg ctggttgacc cgctggatgg cacaaaagaa tttgttaagc
     4321 gcaatggcga atttaccgtc aatattgctc tgatcaaagg caatgaaccg ttagccggtg
     4381 tgatatatat tccggttaca ggagttttgt attttgcctg ccaaacgaca ggcgcacgga
     4441 aaacagttct ggaaccatcc gggaaacaat cttttgatca aataattaaa gattcaacaa
     4501 tcctgccgga aagtttcagc ccaaataaat taacggtcgt ctgcagccgc tcccatatgt
     4561 cggctgaaac ggaaaaatat atcgaccagc taaggcaaac ccatcacgat gtagatttcg
     4621 tatcgatcgg cagctcattg aaattatgcc tggtagcaga aggtaaagca cacatctatc
     4681 cccggtttgg tcctactatg gaatgggata ctgcggcagg acatgccatt gcgttgtttg
     4741 caggctgtac ggtcaggcaa gcagattccg gcgctccggt tgtttacaat aaaccggatt
     4801 tgaagaatcc atggtttatt gtttcgaggt gaaataaaaa gaggctgtcc ggacaattga
     4861 agcagcctct ttcgatatta aattcgtttc ggtttatttt tcgatttcaa ccagttcaac
     4921 ttcaaagacc agtgtggaat atggcggtat atcttcgcct gcgccccgct ccttatatcc
     4981 aagacggtag ggaattacaa gcaaaccttt tgctccttcg ctcatcaggg cgataccctg
     5041 atcccagcct tcaatgacct gcctctggcc cagtgcaaat tcaaatggct ctcccctgtc
     5101 gacggaagaa tcaaatttat tgccgttcaa taacttgccg gtgtaatgaa ctttaacttt
     5161 tttgccggtt tccggttttg gtccgttccc tgcttttgtc tcaatataaa ccaggccgct
     5221 ggggagcgtt gtcgtcggag caagtccgtt atcctttaaa tatttctgaa tgtttgcttc
     5281 ctcttctttt actttcattt cggcctccgc cttctttttt gactcctttt gctgctgttt
     5341 cgcgttgaat tcatctttag aaatgacctt aatcaattca atttcataat ataaagtgct
     5401 gaacggaggg acaatatttc cggctccctg ttctccgaat gccatggctg aagggatgat
     5461 ggcttttgct tttccacctt ctctcatcat acctaatact tcatgaaagc cttcattttc
     5521 gtactggctt ttgtatttaa aatctaccgg ttcgccacgt tcacgcgtgg agaacaattg
     5581 ttcgccgtct aaaagataaa ccgtgtagtg agctgtgagg tatgtctctt ctgccggtga
     5641 ctttcctttg cctttatcag tttcaatata atagatacct gtagatgatg gttccgctgt
     5701 tatgttattt ttctggatat actcctggag ttttattact tcttgttttt caagttccat
     5761 gtttttcaat tttgtctcca tctccatctg ttcctgggtc tggaccttct gaatcgtaac
     5821 atcgaaaatg atctcgctat catcggtaag aggttcgggt aattccggct ggcggaatgt
     5881 tgttttgaaa agtgggccgg ctttcaggat gaacgtagcg ctatcgcctt gatttaatag
     5941 tgaaatacac tcgttaaaat caccctcata ttctgatttt gccacttgaa ggataatggg
     6001 attgggggat tgcttactgt cgaacattag cgaatccttt aatccgtatt tcagatccat
     6061 tgtaacgatc gttccttctc ttacagatgt cgtgtcgtta ttgtcattgt gtattttata
     6121 gtaaaccccg gatttggact tcttatagcc gggatgagtg cctatattac aggatgttgc
     6181 gaaaactgcc agaataacgg ccagggtgat caatgagaaa acatttttta atttcataat
     6241 tatactttta aattcatttc atgctgacaa attccaaatc ataaaccagg gtggccttat
     6301 ggcgtatctt tttctggtcg ccgataaggc caaaagcgag gtgcgaaggt ataataaatt
     6361 ttgccctgtc gccaaccctc atcaataaaa tagcctcctc cagcccggta attacctgcc
     6421 cttttccgac aacaaattcg agggggcctt cggttctggc cgtatatacc gtgtctccgg
     6481 tcaggaacat aacaacgaaa tcgagcctga ccatcctgcc ggtttctcct tttggcccga
     6541 tccctgtgtg agtgatcata taccttaacc ccgttggggt agtttccatg gcccattggt
     6601 ggcgacggat ataatcgtca atttgttggt tttcttctct tacggcttct ttatttgcat
     6661 ggataagcgg ttctttcaga ccgggcaatg cttctttcct gtcggtactt ggattttcgt
     6721 tgcaggaaat ggccagaatt gcggttactc cgatcagtat acagataact ttaagatatc
     6781 tccgatgcat aattttcaac agccagaacg gtcgatttct tctttatatt gttccagggc
     6841 gttctttaga tgtgaaatgg tattttccat cgattcaaag gaatctccac ccgccgcatt
     6901 ccggtgccct cccccattaa aatatttcct ggcgaaaaca ttcatggata aatttccttt
     6961 cgaacggaat gatagcctga tccggtccgt cctttctgta agtaaaacgg cgaaccggat
     7021 ttcatctatg gatagagcaa aatttacaac accttcggta tcacctgtct tatattgaaa
     7081 ccggtcaagt tcggctttgg tcagggcaat gtaggctgtt ttgtattgtg gtaagatggt
     7141 catcttctct ccaaggcaat aacccagcag acggagcctg ctttcagaat atgtatcata
     7201 gacgtttcgg tgtatctgct ccggatcgat tcccgttttt atcaacaggg ctgatatttg
     7261 aaaggtttca ggataattac atgagtaact gaatgatcct gtatcggtca ttattcctac
     7321 aaaaagcccg gtagcaacct cctgtccgat caaatcagac caaccgcact gctcgataaa
     7381 attgaaaagc aattccgaag tggacgagat atcgattctg gatataatat agtcaaagtc
     7441 ttctgacaag ggatcaagat gatggtcaat caggacttta acagcacggg cgctttttaa
     7501 agcgggtgcc atggcgtcgg tgcggtgaag tgcattataa tccaggcaaa agatcaggtc
     7561 agcattataa atgagttctt tggccttggg ctccaactgg ttgtatatga ctatccggtc
     7621 ggctccgggc atccatttta agaaatcagg aaagtcatta gggacgatac ccgtcacctg
     7681 cggaactctc ttctccaaaa gcttgaccat ggcaataaca gaaccgatgg catctccgtc
     7741 aggattgtga tggctggtca ccacaaccga acggctgtct ttaataatgc ttttgattat
     7801 ttcgatatcc ggatgtttca aaaaatcgtt cttttagtgg gcaaagtaaa tgaaaatatt
     7861 cctgatttcc ttatttttgc gcgctcaaac ttaaatcata tgaatggaaa tatcacatta
     7921 accatgatca aaccggaggc cgtaagaaac ggtcacaccg gtcatattct cgcaaaaatt
     7981 actgaaggag gatttggaat tgttgccatg aaatatctga aattaacgcg tgaacaggct
     8041 gaaaagttct atgaagttca tcgcgaaagg cctttttata aagacctggt cgaattcatg
     8101 acatccggcc cgatcgtagc cgccattttg aagaaggaca acgctgttga agaatacagg
     8161 tcgtatattg gttcgacaaa tccgaaagac gcggccccgg gcactgttcg ggcactttat
     8221 ggaacaaaca ttcaggagaa tgccgtacac ggttccgaca gcgatgaaaa tgctgcccgg
     8281 gaagcctcct tcttcttcag cgaattggag cgttattgat cttttccgga ttccggtttc
     8341 agtacccgga aggtgccatt gcggaaaatc ataataatct gttcaaccgg ttcatcatcc
     8401 ttatgaaccg gttttttttg tatttcgtta ttctcttctg cttcaaagag agtaggcggt
     8461 aaggataccg gaacagtttt gctaactgcc ggacttgtac tcaaaccgga agtagctgac
     8521 tttttccccg aaaccagcca gttcaggtct gcctccggga aggcagtttt gattttttca
     8581 aggaagtcaa tactgggttt attcctgccg gaaaggatat gggaaatgct ggaacgctga
     8641 accccgattc tgtctgcaaa ttccgaggcg cttaatccct ggtctgccat tagttgcctg
     8701 aacctgtcga tcatgctttc attctatatt acaaatgtaa tgcaatattg tttacatgag
     8761 taatacattt gtaaacatta aatatccccc atacacaatc taatttactg taatacagca
     8821 cttatctaca actaattaca attgtaattt acaaaagtaa acatttgtaa attggtagtt
     8881 atatcacatt tgtatttaaa ctaattattc cgattaggat attcaatatg ctatattcca
     8941 gatattagat gcatatatgc tttcttctgg ctttaattgc ggagtattgt tctttgtttt
     9001 ctactaagaa acacggggaa agaaaactgg aagtcaaaac gaaaggtccg ggcagaaata
     9061 tacctgcccc ttccctattt atgtgaggga gaattatctt tattaaaaaa aatatgaagg
     9121 taaattaccg tttgatgaat tttccggtaa aagttcttcc gttcgcagac acactcacaa
     9181 aataaactcc agcctcaagg ccttttgcgt tgacagtata ttcattaccc attaacgaag
     9241 ctgtgtaaat ttctcttccg gtgatgtctg taataatgat atcggcatta ttggcattaa
     9301 cctgatcaag gctgactgta aacgatgtcc tgacgggatt agggtataga tttaaactta
     9361 cgtgttgttt gtcagggcca ttgatgaccg ttagtatatt attggccagt acaaaatcag
     9421 gaatgccgta gcccagcatg tcatcgggat tatcgtactg actggcactt ttcctgatgg
     9481 catcggcaat ttgcatgtta gtcatggctg gatttgcctg ccacagacag gcagccattc
     9541 cagccaggat gggtgatgag aaagaagtgc cgcctccaaa agtaaatcca ccgcccatcg
     9601 gttccaccac gtataccatt gatccttgtg tcgccacatc cggttttacc ctgccatcgt
     9661 aagaagggcc atgggagctg aaacccacat aaaaaccatt ggcgtccaca gcccctattc
     9721 ccattacgct gtcgccatca gaaggcgctg ataaatagta ccatgagcta ttgccttcat
     9781 tacccaggct atttacgacc aatattcctt ttttcgcggc catatcggcg ccttttgtgg
     9841 cgggagtcgt atttccgtcc atatcttcgt aggtatgatc ctgggtcggg tcgttgaatt
     9901 gagaatagcc cagtgatgaa ttgataatgt cggccccaac gctatcggca aattccgcgg
     9961 cagataccca gttgtattct tcgatcaagt attccgatcc accgtcctca gaccgcagaa
    10021 gccagtaatc ggccattggt gccgtcccga tcagttgccc tgggaaattc gctcccatgc
    10081 aggaaagcac cattgtcccg tgctggtgtt catcaaactg aatatctcct cccctgacga
    10141 aatcctttgt tcccaggatc tggccgttca tccagagact atcgaaagca tctacactgt
    10201 tggcattaaa atatccggca tccagaacgg ctataacctt cccctgtccg cggtaaccca
    10261 tttcgtgaaa aacatctccg ttcagcatct ggatctggtt aaatcccatc ccataatcaa
    10321 atg
//
LOCUS       DAOPNH010000099        10286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7014_length_10286_cov_6.514319, whole genome shotgun sequence.
ACCESSION   DAOPNH010000099 DAOPNH010000000
VERSION     DAOPNH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_10286_cov_6.514319"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3028
                     /locus_tag="PKZ74_06665"
     CDS             <1..3028
                     /locus_tag="PKZ74_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM92478.1"
                     /translation="TFVLLVGDAAQVPASSTSAGDSDNNYAYVVGSDHYPDLFIGRFS
                     AENVNQVQTQVQRTIMYEQNPPVSADWFSVCTGIASNQGPGDDNELDYEHIRNISNNK
                     LIPFTYTFANELFDGSQGGNDEGGNPTPTQVAADVNAGTSIINYTGHGSDGSWGTSGF
                     SISDVNNLVNDNLLPYVWSVACVNGNFVGGTCFAEAWLRATHNGNPTGAVAFLGSTIN
                     QSWDPPMAGQDEMNDILVETYPNQINRTFGALSMHGCMLMNDEFGSAGVEMTDTWTCF
                     GDPSVMVRTAMPVTMTVTHDPVLFLGATQTMILCDHNGARATLSLNGTRLATGVVANG
                     SVTLEFAPLGEIGTADLTITAFNTLPYMMGIDIIPATGPYVVMNSYQIADEAGNNNGL
                     PDYNESVGMAVELENVGIQDATDVLVTLVTDDPYITITDYSETYPLIPAGSTASVEDA
                     FAFSISSDVPDQHQVMFTITSIMGDETWQSIFMLNANAPILNINSITILDSEEGNGDG
                     ELDPGERADLVINYNNTGHAVAYDVNLFMEGQSGFVEISNPDQNFTSIGFFGVFNKTY
                     TVVVDEDVPEGIKIDFVNELTMGDFMMNRVFPLRVSAKVEDFETGNLEQFNWQTGGNA
                     AWQVVNSYPYEGFYCLKSGTISHSQTSEISLVYNVMEADSIIFYRKVSSESSDQLKFY
                     INNQVAGSWSGTTGGWKREAFAVSAGQKTFKWAYEKNNMGSGGSDCAWLDYIILPSPF
                     ALTIWAGSDNSVCNGNSYEINDAYGTDYELIEWISSGSGTFDDNTSMHPLYTPSGQDL
                     STGQVALTLNLWDDEGSMVTDEMQLGFRDVPSAPEQPDGLSYVDVATVETSDYTTDIM
                     EEADDYNWTIDPPVAGVIIGNENTATVSWNAEFTGVAYISVTGGNACGNGEVSAALEV
                     QVVNSIVGLTEPDDPAVSILPNPASGIIHVNLYSIGSYNPVVRLVNMIGIELFNSGVV
                     NNVSDIVIPVSGYAPGVYLLSVNTGDRQLTRKIIIK"
     gene            3028..>4502
                     /locus_tag="PKZ74_06670"
     CDS             3028..>4502
                     /locus_tag="PKZ74_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM92479.1"
                     /translation="MIYRICAFSVILILNLSAGGQNRVTGISLLNYTGEIRYVPDVMG
                     FVVVADNLNLSVDQKQIQRDEIILFPGWPVSIGGSNERGGVYANLDGDEDFELIYPAG
                     TMLYALNIDGTAVEGWPRQLDHPTDGSAAFGDIDGDGAGEIVVTTHENATFAIGSVYA
                     FETDGTMVTGFPVATEGGGIRTPALSDLDLNGALEIVMTVRKWPDGFVYVFKGDGTMF
                     PGWPQRMDYVPGSAAAAGDIDGDDYPEIIAESYYRLHAYNRFGDLLPGFPYLPGENRV
                     FSYSTPVLADLDEDGNREIICGDHSIGDGTGAVHIVRHNGLSLPDWPRFTVSWVYSPP
                     SVADIDGNGLLDVAVGDQTLSETPVNRIYAWTAMTGEALPGFPVENVHGINSQILLAD
                     VDGDGMVELITDDNTSNGKYAGFNHDGTVAEGWPLSLDGTTFFINPFVTDINFDGTLD
                     ISGAGYIVGAGLTNVYLWNTGKDYVEENNPLPILQYNTRRNG"
     assembly_gap    4503..4602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4603..5192)
                     /locus_tag="PKZ74_06675"
     CDS             complement(<4603..5192)
                     /locus_tag="PKZ74_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92480.1"
                     /translation="MKTRAFLLVMLVMGFFALSFGQEQPVQDMKTLFKPTGKKISHGG
                     YIGVSVGYTQMKNENVITLGGRAGWLIDHHVTIGIAGNSFISNPYIKSNFPNTEDTAF
                     YLMGGYGGLLIEPIIAPNFPVHVSFPILIGGGGFILDDQSIFHRDSYEYGDGDYNYDY
                     YGYYNWDSFFVVEPGVEIELNVIKFFRIGVGASYRIT"
     gene            complement(5270..5797)
                     /locus_tag="PKZ74_06680"
     CDS             complement(5270..5797)
                     /locus_tag="PKZ74_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92481.1"
                     /translation="MKISAKLLIVTLFMLSVIQAGAQENRKQAGFRFGNTTGFSARVI
                     TEDDFAFEGILGFRNGGLQLYGLFESRKPLYFNRIENMHLYFGGGVHVGFVRWNDYDK
                     YYDPYGNDHYYDDYYKWHTGMAFGLDGIVGMEYVFQAAPISLAVDFKPFFEMYGPFLM
                     RVNFWDFGFHMRYNF"
     gene            complement(5822..6598)
                     /locus_tag="PKZ74_06685"
     CDS             complement(5822..6598)
                     /locus_tag="PKZ74_06685"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPM92482.1"
                     /translation="MKTKKFSVLLLLFAAGLFFTGCYKPWHVIEGNHDVQTDTRAISG
                     FSHVFNEGNFDVYIIQDASGEVIIEAESNLIPLIRTRIEGSALVIDTRDNLHNNYPMK
                     VYVHTPEIEKIRLSGSGLMHAENINTGDIDIDLSGSGDIFFSGIADDLECKISGSGDM
                     EIGIECNTIDAKISGSGEMEFYGSAHRGDLNISGSGSVRAYDLTLQDCYARISGSGDM
                     YLTVEDYLNVTISGSGDVYYLGNPIIETNISGSGNVIHPR"
     gene            complement(6669..7772)
                     /locus_tag="PKZ74_06690"
     CDS             complement(6669..7772)
                     /locus_tag="PKZ74_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92483.1"
                     /translation="MTLLSNYKYLLVIACMAFSFPLAAQVYKNNRTVREAFRIAPNTE
                     VQVINKYGDIHLIPWEKDSVVFQIELSVTSNKQSKVDKIYDYIDFDFKSTAYYVIAQT
                     VFEGQNTIWSEMADVASTIFSSGTNTRIDYTVYFPAGNDVRIENKFGNIYTTDHTGKV
                     DFTLSNGDLKSHSFSGPTRLKIEFGNATIDKIKNATIVLGYAELNLDMAEDLTFDSRS
                     SKVYINNCKKVHLDSKRDRFQVKSAGEITGETFFSYLNLDYVNSRINLKTSYGDLKLL
                     QVAPAFVRMDIHSQYSDVTVYYDEERLYEFDITRDDRSQVIAPATVLSKKEDPVAGME
                     KTFRAVITSGNINKSKVPIIINIKSGKIYLVES"
     gene            complement(7750..8322)
                     /locus_tag="PKZ74_06695"
     CDS             complement(7750..8322)
                     /locus_tag="PKZ74_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92484.1"
                     /translation="MSPDKLEKYMLEHRQEFDDMEPDPSLWERIDTRKAPVIRPNWMS
                     IGWKVAAGVAIFIASYFFHDYMSSRNNNQESFMAGLNEEASPIARELIEAEAYYTARI
                     SYTKDEVFRLAAADPDLRREVDLQMVDLDRVFTELKEDLKDNAANEEVIEAMIQNYRL
                     KLDILEDMLRQLKESKEPQNDTSHDAAIEL"
     gene            complement(8312..8890)
                     /locus_tag="PKZ74_06700"
     CDS             complement(8312..8890)
                     /locus_tag="PKZ74_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92485.1"
                     /translation="MDNPIRLSNVEQADISRDIIERSKKGNRQAQYQLYRQYAKAMFN
                     ICNRMMNDRADAEDMLQESFSEAFRRLDSFRNESTFGAWLKMIVINRCINEIKRKKAQ
                     LEFFDDMSPFEDEEDLTDDQSAGLSPERIKKAMEDLPKGSRMIFSLYLLEGYDHQEIS
                     EILNISESNSKTQYMRAKQRIKEILKNQPYES"
     gene            9130..>10286
                     /locus_tag="PKZ74_06705"
     CDS             9130..>10286
                     /locus_tag="PKZ74_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPM92486.1"
                     /translation="MKLPFAESYKIKMVESLYRSTKSEREQWIREAKYNLFNLRSGHV
                     FIDLLTDSGTGAMSDLQWSAMMLGDESYAGASSYFKMKEAIQTVLGFDYFLPTHQGRA
                     AENVLFSVLVKSGDIIPGNAHFDTTKGHIEFRHATAIDCTIKEAYDTTVHHPFKGNID
                     LEKLEETLKAHPAEKIPMIIVTVTCNTPGGQPVSMENLKAVSALAKKYATLVVIDGAR
                     FAENAYFIKLREPGYEHISIRDIVKEMMSYADAMTMSSKKDAIVNMGGFIGFRNEELF
                     RSASVFNILFEGYITYGGMSGRDMNALAQGLYEGTEFNYLETRVEQVAYLGKKLVEYG
                     IPVQQPFGGHAIFVDARRFLPNVPKEQFQAQTLAVELYLEGGVRGVEIGTLL"
BASE COUNT         2555 a         2501 c         2502 g         2628 t
ORIGIN      
        1 gactttcgtg ctgctggtgg gcgacgccgc tcaggtgccg gcatcatcta cctctgccgg
       61 cgattccgat aacaactatg cttatgtcgt tggcagcgat cattaccccg accttttcat
      121 cggccggttc tccgctgaga acgtgaacca ggtacagaca caggtgcaac gaaccatcat
      181 gtacgaacag aacccgccgg tctccgccga ctggttctcc gtctgcacag gcatcgcctc
      241 aaaccagggt ccaggcgatg acaacgaatt ggactacgaa catatccgca atatctccaa
      301 caacaagctg atccccttta catacacttt tgccaacgaa ctgttcgacg gcagccaggg
      361 cggcaatgac gaaggcggta acccgacacc gacgcaggtg gcagccgacg tgaacgccgg
      421 taccagcatc atcaactaca ccggtcacgg cagcgacggt tcatggggca cctcgggatt
      481 ctcgatcagc gatgttaaca acctggtcaa cgacaacctg ctgccttacg tatggtcggt
      541 agcctgcgtg aacggtaatt ttgtcggtgg tacctgcttc gccgaagcat ggcttcgcgc
      601 aacacacaac ggcaatccca ccggcgccgt ggctttcctg ggatctacca tcaaccagtc
      661 gtgggatccg ccaatggccg gccaggatga aatgaacgat atcctcgtgg agacctaccc
      721 gaatcagatt aaccgcactt tcggtgcctt atcgatgcat ggctgtatgc tgatgaacga
      781 cgagttcggc agcgccggcg tcgaaatgac cgacacatgg acctgtttcg gcgaccccag
      841 cgttatggtg cggaccgcca tgccggtcac tatgaccgtg acacatgatc cggtcctttt
      901 cctgggggca acccagacca tgatcctctg cgatcacaat ggcgccaggg ccacgctgag
      961 cctgaacggc acccggctgg ctaccggtgt ggtggccaac ggatcggtca cactggaatt
     1021 tgcgcccctg ggtgaaatcg gcacggccga ccttactatc accgcattca atactttgcc
     1081 ctatatgatg ggaattgata tcatcccggc taccggacct tatgtggtca tgaattccta
     1141 tcagattgct gatgaggccg gaaataacaa cggcctgccc gattacaacg aatcggtcgg
     1201 aatggcagtg gaactcgaaa atgttggtat tcaggatgca acggacgttc tggtcaccct
     1261 ggttacggac gacccttaca tcacgatcac cgattatagc gaaacctatc cgcttatccc
     1321 cgccggaagt actgcatcgg tggaagacgc ttttgctttc tcgatttcca gcgatgtgcc
     1381 cgaccagcac caggtcatgt tcaccattac atccatcatg ggcgacgaaa cctggcaaag
     1441 catttttatg ttgaatgcca atgcccctat cctgaatatc aactcgatca cgatcctgga
     1501 ttcggaggag ggcaatggcg acggagagct tgatccgggc gaaagggctg accttgttat
     1561 caattacaat aataccggcc atgccgtagc ttacgatgtg aatcttttta tggaaggaca
     1621 atcgggcttc gtggaaattt ccaaccccga ccagaatttc acatcaattg gcttctttgg
     1681 cgtttttaac aagacctata ccgtggttgt ggatgaagac gttcccgaag ggataaaaat
     1741 tgatttcgtg aatgaactga caatgggtga tttcatgatg aaccgtgttt tcccgctgcg
     1801 ggtgagcgca aaagtcgaag actttgaaac gggcaacctg gagcagttca actggcaaac
     1861 cggcggtaat gctgcctggc aggtggtgaa ttcatatccc tatgaaggat tctactgcct
     1921 gaaatccggt acgatatccc atagccagac atctgaaatc tcactggttt acaacgtaat
     1981 ggaggccgac agcatcattt tctaccgtaa agtttcttcc gaatcatccg accagcttaa
     2041 gttttacatc aacaaccagg tggccggaag ctggtcgggt acgacagggg gatggaagcg
     2101 ggaagcgttt gctgtcagcg ccggccagaa aactttcaag tgggcttatg aaaagaacaa
     2161 catgggcagc ggcggttccg actgcgcctg gctcgattat atcatcctcc catcgccctt
     2221 tgccctgacg atctgggccg gaagcgataa ctcagtatgc aatggcaaca gttatgaaat
     2281 taacgatgcc tatgggaccg attatgagct gattgaatgg atctcctcgg gaagtggaac
     2341 cttcgacgac aataccagca tgcatccgtt gtatactcca agcggccagg atcttagcac
     2401 cggccaggtt gcgctgacac tgaacctgtg ggacgatgaa ggcagcatgg tgaccgatga
     2461 aatgcagctc ggattccgtg atgtcccatc agctcctgaa caacctgatg gattgtctta
     2521 tgttgatgtt gccacagtgg agacttctga ctatacgaca gacataatgg aagaggctga
     2581 tgattacaat tggactattg atcctccggt ggccggcgtg atcatcggga acgaaaatac
     2641 ggcgaccgtg agttggaatg ccgaatttac cggtgttgca tatatttcgg tgaccggcgg
     2701 caatgcatgc ggcaacggcg aagtttcggc tgccctggag gtgcaggtgg ttaattccat
     2761 cgttggttta acggaaccgg atgatccggc tgtttccatc ttgcccaatc cggcatccgg
     2821 aatcattcat gtgaaccttt attctattgg aagttacaat ccggttgttc ggctggtgaa
     2881 catgataggg attgagttgt ttaattcggg agtggtgaat aatgttagcg acattgttat
     2941 tcccgtttcc ggatatgctc cgggagttta tctcctctcg gtcaataccg gtgaccggca
     3001 attgacccga aaaattatca taaaataatg atctacagga tatgcgcttt ttcggtcatc
     3061 ttgattctga atctctccgc tggcggacag aatagagtga ccggcatatc cttgctgaac
     3121 tataccggtg aaatccgcta cgttcccgat gtcatgggat ttgtggtggt ggcggataat
     3181 ctcaatcttt ctgttgatca gaagcaaatt cagcgtgacg aaatcatcct ctttcccggc
     3241 tggcctgtgt cgattggtgg aagcaatgaa aggggagggg tttatgccaa cctcgacggt
     3301 gatgaggatt ttgagttgat ttaccctgcc ggaacaatgt tatacgcatt aaatatcgac
     3361 ggaacggcag ttgaagggtg gccccggcaa ctggatcacc ctaccgacgg atcagcggct
     3421 ttcggggata tcgatggcga tggagcgggc gagatcgtgg tgaccaccca tgaaaatgcc
     3481 acctttgcta tcggatcggt ttatgcgttc gagacagatg gaacaatggt aaccggattc
     3541 ccagtggcga ccgagggcgg cgggatccgc accccggccc tgtcggacct cgacctcaac
     3601 ggagcgctgg aaatcgtgat gacggtcaga aagtggccgg atggcttcgt ctatgttttc
     3661 aaaggagatg gaacaatgtt cccgggctgg ccgcaacgga tggattatgt ccccggttcg
     3721 gcggctgcgg caggagatat cgacggggat gattaccccg agatcattgc agaatcgtat
     3781 taccggctgc atgcctacaa ccgtttcggc gacttattgc ccggattccc ttatcttccc
     3841 ggcgaaaacc gggtattttc ctattccacg ccagtattgg ccgatcttga cgaagacggt
     3901 aaccgtgaga tcatctgtgg cgatcatagt atcggggatg gaacgggtgc cgtgcatatt
     3961 gtacggcaca acggcctttc attacccgac tggccaaggt ttaccgtaag ctgggtctac
     4021 agcccgccgt cggttgccga tatcgacggc aacggcctgc ttgatgtggc tgtcggcgat
     4081 cagacactaa gcgaaacccc tgtgaaccgg atatacgcct ggactgccat gaccggcgaa
     4141 gcgctgcctg gatttcccgt tgaaaatgtc cacggtatca acagccagat cctcctggcc
     4201 gatgtcgatg gcgatgggat ggtcgaactc attaccgacg ataacacctc gaatggaaaa
     4261 tatgccggtt tcaaccacga cggaaccgtt gccgagggct ggcccctttc actggatggc
     4321 actacctttt ttataaatcc cttcgtaacc gatatcaatt ttgacggaac tctcgacatt
     4381 tccggtgcag gatatattgt gggcgccggt cttactaacg tttacctctg gaataccggc
     4441 aaggattatg tggaagagaa caatcccctt cccatattgc aatacaatac ccggcgcaac
     4501 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtaattct gtatgatgcg
     4621 cccacaccga tgcggaagaa cttgatcaca ttgagttcga tttcaactcc cggttcgacc
     4681 acaaagaatg aatcccagtt ataataacca taataatcat aattgtaatc accatcgccg
     4741 tattcatatg agtccctgtg gaagatcgac tggtcgtcaa gaataaatcc acccccgccg
     4801 atcagaatcg ggaaggagac gtggacgggg aaattcgggg cgataatggg ctcgatcagc
     4861 aggccaccgt aaccgcccat caggtaaaac gccgtatctt ccgtattcgg aaaattgctc
     4921 ttgatgtaag gattgctgat gaaggaattc ccggctatgc caatggtaac gtgatgatcg
     4981 atcaaccagc cggcacgccc gccaagggtg atcacatttt catttttcat ttgggtatag
     5041 cccaccgaaa caccgatata gcccccatga ctgatcttct tgcctgtagg tttgaataat
     5101 gttttcatat cctgtaccgg ctgttcctgt ccgaatgata aggcgaaaaa tcccatcacc
     5161 aacattacga gtaaaaatgc tcttgttttc attattttag tttgttaaat ttctgaaaaa
     5221 tatcttaaat actttctaat taagaattaa gaactaaaaa tttaaaattt taaaaattat
     5281 atctcatatg aaacccgaag tcccagaaat tgacacgcat caggaacggc ccgtacatct
     5341 caaagaaagg tttgaaatcg acggctaaac tgataggtgc agcctggaag acatattcca
     5401 tacccacgat cccgtcgagc ccgaaagcca taccggtgtg ccatttgtaa taatcatcgt
     5461 aataatgatc gtttccgtaa ggatcatagt atttatcgta atcattccag cggacaaaac
     5521 ccacatggac acctcctccg aaatacaggt gcatattttc gatcctgtta aaataaagtg
     5581 gttttcttga ttcgaacagg ccgtacagtt gcagtccccc gttgcgaaaa cccaggatgc
     5641 cctcaaaggc aaaatcatct tccgtgataa cccgggctga gaatcccgtc gtattgccaa
     5701 accggaaacc ggcttgcttc ctgttctcct gcgctccggc ctgtatcacc gaaagcataa
     5761 agagggtgac aataagtagc tttgctgata tcttcataat tatcaggatt ttatgaggtt
     5821 attaccgcgg atggataacg tttcctgacc cggaaatatt cgtttcgata ataggattgc
     5881 ccaggtaata gacatcaccg ctgcccgaaa tggtgacatt cagataatct tcaacggtca
     5941 ggtacatatc gccggaaccg gagatccgtg cgtagcaatc ctgtagagtc aggtcatagg
     6001 ccctgaccga tcccgaaccg gagatattca ggtcgccgcg atgggctgat ccgtaaaact
     6061 ccatctcccc cgaaccgctg atcttcgcat caatagtatt gcattcgatc ccgatttcca
     6121 tatcgcccga acccgatatc ttacattcca ggtcatctgc aatacctgaa aaaaagatgt
     6181 cacccgaacc gctcagatcg atatcgatat cgcctgtatt gatgttttcg gcatgcatca
     6241 gtcccgatcc gctcaacctg atcttctcga tctcgggcgt atgcacataa actttcatgg
     6301 ggtagttgtt gtgcaggttg tctctcgtgt cgatgaccag ggccgaccct tcgatccggg
     6361 tccggatcag cggaattagg ttggattcgg cttcgatgat cacttcgcca ctggcatcct
     6421 ggataatata cacatcaaaa ttcccttcgt taaacacatg gctgaaaccg gaaatggccc
     6481 gtgtgtcggt ctgaacatca tggttccctt caataacatg ccagggcttg taacatccgg
     6541 tgaagaataa ccctgccgca aacagtaata acaatacgct gaatttcttt gttttcataa
     6601 tttattctta ttttgatttt tttattaaag acaagataaa ggacaatagg ttactttttt
     6661 tttaaaaatt atgattccac caggtaaatt tttcccgatt taatattgat gatgatggga
     6721 acctttgatt tattaatgtt tccggaagtg atcaccgccc tgaatgtttt ttccattcct
     6781 gccacaggat cttctttttt gctgagtaca gtagcaggag caatgacctg tgaccggtcg
     6841 tcgcgcgtta tatcgaattc atagagcctt tcctcgtcat agtaaaccgt cacatcggaa
     6901 tattgcgaat ggatatccat cctgacaaat gccggggcaa cctgtaaaag tttgagatca
     6961 ccgtagctgg tcttcaggtt gatccgggag ttcacataat cgagattcaa atatgagaaa
     7021 aaagtctccc cggttatttc tccggctgat ttgacctgga atcggtcgcg cttggaatcg
     7081 aggtgcactt tcttgcaatt gttgatataa acctttgaag accggctgtc aaaagtgaga
     7141 tcttccgcca tatcaaggtt aagctcggca tagccaagaa ctatggttgc gtttttgatc
     7201 ttgtcgatgg tagcattgcc aaactcgatc ttcagtcgtg tcgggccgga gaaactgtga
     7261 ctcttcaggt cgccgttcga cagcgtaaag tcaactttcc ccgtatgatc ggtcgtgtag
     7321 atatttccga atttattctc gatcctgaca tcgttaccgg cgggaaaata gaccgtgtaa
     7381 tcgatgcgcg tattggtacc gctgctgaaa atagtgctgg ccacgtcggc catctcgctc
     7441 cagatggtgt tctgcccttc gaatacagtc tgggcgatta cgtaataagc agtcgatttg
     7501 aagtcgaaat cgatataatc ataaatttta tcgactttcg attgcttgtt cgacgtaacc
     7561 gacagctcaa tttgaaagac caccgaatcc ttttcccagg gaataagatg gatatcaccg
     7621 tatttgttga tgacctgcac ctcggtattg ggtgcgatcc ggaaggcttc cctcacggtg
     7681 cggttgttct tgtaaacctg cgcggccagc gggaagctga aagccataca ggctattaca
     7741 agaaggtatt tataattcga tagcagcgtc atggcttgtg tcgttttgtg gttcttttga
     7801 ttctttcagt tgcctgagca tatcttccag tatgtcgagt ttaagacggt aattctggat
     7861 catggcttcg atgacttctt cattggcggc attgtccttc agatcttctt tcaattctgt
     7921 aaataccctg tcgaggtcaa ccatttgaag atctacctcc cgccgaaggt caggatcggc
     7981 ggcagccagc cggaacactt cgtctttggt gtaactgatc cttgccgtgt aatacgcctc
     8041 tgcttcaatc agttcgcggg cgatagggga ggcctcttcg ttaagtccag ccatgaagct
     8101 ttcctggttg ttgtttctcg acgacatata atcgtggaag aaataagaag caatgaagat
     8161 ggcgacgccg gcggcaactt tccatccaat gctcatccag ttgggccgga tgaccggggc
     8221 cttacgggtg tctatccttt cccagagcga tgggtcaggc tccatgtcgt cgaattcctg
     8281 acggtgctca agcatgtatt tttccagttt atcaggactc atatggctga ttttttaata
     8341 tttctttaat ccgttgtttg gcgcgcatgt actgtgtctt ggaattggat tcgctgatgt
     8401 tcagtatctc cgagatctcc tggtggtcat agccttcaag caggtaaagc gaaaatatca
     8461 tccggcttcc tttgggcaga tcttccatcg cctttttaat ccgttcgggc gataacccgg
     8521 ccgactggtc atcggtcagg tcctcctcat cctcaaacgg cgacatgtcg tcaaagaact
     8581 ccaactgggc cttttttcgc ttgatctcat tgatgcaccg gttgatcacg atcatcttga
     8641 gccaggcgcc gaaagtggat tcgttgcgga aactgtcgag ccgccggaat gcttccgaaa
     8701 acgactcctg cagcatatcc tcggcatcag cccggtcgtt catcatccgg ttgcaaatat
     8761 tgaacatggc tttggcgtat tgccggtaaa gctggtactg cgcctgcctg ttcccttttt
     8821 tgctccgctc gatgatgtct ctgctgatgt cagcctgctc cacgttcgat agccttattg
     8881 gattatccac tgaaaaagac aataaaaaaa ttaagaggtt gcaatttttt taaaagtaaa
     8941 aaaaatattt atttttgacg gtcaggttat ttcattaatt aatgatttac aggcaattca
     9001 tatctgataa acttatctac ctgaatataa aaaacaggga cgaattccgg attccaggtt
     9061 ccggattcca tgtttaaagt tattttatca gttaattcag aattaagaat ttaaaattca
     9121 aaattgtcta tgaagttgcc tttcgcagaa tcatacaaga taaaaatggt cgaatccctg
     9181 taccggagta ccaaatcgga aagggagcag tggatcaggg aagcaaagta caatctcttc
     9241 aatcttcgta gcgggcacgt gtttattgac ctgttgaccg actcagggac cggcgccatg
     9301 agcgacctgc agtggtcggc catgatgctc ggcgatgaaa gctatgccgg cgcctcttcc
     9361 tatttcaaaa tgaaagaagc gatccagacc gtcctggggt ttgactattt tctgccgact
     9421 caccagggcc gggcagctga aaatgtcctc ttttcagtcc tggtaaaaag cggcgatatc
     9481 atcccgggaa atgcccactt cgatacgacc aaaggccata tcgaattccg tcatgccaca
     9541 gccatcgatt gtactatcaa agaagcttat gatacaacgg tccatcaccc gttcaaggga
     9601 aatatcgacc tggaaaaact cgaagaaacc ctgaaagctc atccggcaga gaaaatcccg
     9661 atgatcatcg tgacggtgac gtgcaacact cccggcggcc agcccgtttc catggagaac
     9721 ctgaaagcgg tatcggcatt agccaaaaag tacgcgacac tagtggttat cgacggagcc
     9781 cggtttgcag agaacgccta ttttatcaag ctgcgcgaac cgggatatga acatatttcc
     9841 atccgtgata tcgtgaagga gatgatgagc tacgccgatg ccatgaccat gagcagcaag
     9901 aaggatgcaa ttgtcaatat gggcggattt attggttttc ggaatgaaga gttgttccgc
     9961 agcgccagcg tgttcaatat tctttttgaa ggatacatca cctacggcgg aatgtcgggg
    10021 cgcgatatga atgccctggc acaggggctt tatgaaggga ccgagttcaa ttacctggaa
    10081 acacgggtgg aacaggtggc ttacctcggg aagaaacttg tggaatatgg tattcctgtg
    10141 caacaacctt ttggcggaca tgctattttc gtcgacgccc gccgcttctt gcccaatgtc
    10201 ccgaaagaac aattccaggc tcagacgctg gccgtggagt tgtatctgga aggaggagtg
    10261 agaggggtgg agattggcac cctgct
//
LOCUS       DAOPNH010000100        10277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7027_length_10277_cov_6.131970, whole genome shotgun sequence.
ACCESSION   DAOPNH010000100 DAOPNH010000000
VERSION     DAOPNH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7027_length_10277_cov_6.131970"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKZ74_06710"
     CDS             <1..480
                     /locus_tag="PKZ74_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPM92487.1"
                     /translation="VTAFANLVISLIRNGIPPRIRIIVQLTVVATLVILVDQLLKAYV
                     YDVSRQLSVFVGLIITNCILMGRLEAFAMANKPWPSFLDGIGNGLGYSWILIALGFVR
                     ELFGSGTLWGYPVLPQALYDLGYKNNGLIILPPMALILVGLIIWIQRSRTPSLIDKS"
     gene            507..1123
                     /gene="nqrE"
                     /locus_tag="PKZ74_06715"
                     /pseudo
     CDS             507..1123
                     /gene="nqrE"
                     /locus_tag="PKZ74_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
     gene            1155..2396
                     /gene="nqrF"
                     /locus_tag="PKZ74_06720"
     CDS             1155..2396
                     /gene="nqrF"
                     /locus_tag="PKZ74_06720"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPM92488.1"
                     /translation="MGLVIGISIVVFLVIILLLVGVLLYARAKLIPQGKVKISINDDK
                     ELEVSPGSSVLSTLANSGIFLPSACGGGGTCAMCKCQVLEGGGSILPTETGYFTRKEQ
                     QNHWRLGCQVKVREDMKLHIDPHIFGIKKWECEVVSNKNVATFIKEFVVKLPEGENLD
                     FKSGGYIQIDVPVCEVDYGKDIFVEEEYRDEWDKFKMWELKMKNPEPVFRAYSMANHP
                     AESNIIMLNIRIATPPFDRKSGGFMNVNPGICSSYVFSRKPGDKVTISGPYGEFFIKD
                     TRREMMFIGGGAGMAPMRSHIFDQFNTKHSDRKATFWYGGRSLRELFYIDEFREIEKN
                     NPNFKFHVALSEPKAEDNWTGYTGFIHQVIIDNYLKQHPEPEEIEYYICGPPMMNAAI
                     FKMLDDYGVPKEMIAFDDFGG"
     gene            2445..3224
                     /locus_tag="PKZ74_06725"
     CDS             2445..3224
                     /locus_tag="PKZ74_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92489.1"
                     /translation="MKKVLLFTLLSVFGFMTISFAQDVIYKKDGSKEEVKVTLVGEKE
                     IQYKKFSNPDGPVYSLPKHEIVLITYENGEFETIVPKESAGMPVKTDFTVNFAKNIIS
                     YHMFDLVFGDFTFSYERLLNNGMVGFKIPVAMGYYYYSDFGNFNSIIYSGLGVNFYPT
                     GQGRWRYFVGPQVRAGIGEENEWIEYYDDYGNYLYSDDIENRGFYTQFFIDNGVTFLP
                     VRNFGISVIASIGVRYFPEASYDSDALRPDGQFAVNISYRF"
     gene            3232..4233
                     /locus_tag="PKZ74_06730"
     CDS             3232..4233
                     /locus_tag="PKZ74_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPM92490.1"
                     /translation="MTRLSLFGLILMLLSVSCFTPDKKVMLRGEAQGTYYAITYFDRT
                     GRNFQPEIDSILRRFDLSVSLWEPGSILSRINNGDSLALPDEWFTDIFNRSVDIAEKT
                     GGAFDFTVGPLVNAWGFGFRNKIRLDSAKVDSLLELVDFRTVGLYEGKIVKRDPRVQF
                     DFNAIAQGYAVDVLGNFLASEGISNFLIDIGGEVLGKGQKPDGSDWLVGIENPSTDSL
                     SERTLNARVKLSGKALATSGSYRKYYEENGVRYSHTIDPKTGYPVRHSLLSVSVLAGD
                     CATADGYATAFMVMGFEKAKDFVENEPELEAYFINASEDGLFETYATKGFRSLLISD"
     gene            4315..4518
                     /locus_tag="PKZ74_06735"
     CDS             4315..4518
                     /locus_tag="PKZ74_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92491.1"
                     /translation="MLNVILITIILVGLAFAGFAIKILLTKKGEFKKSCSSVDPVTGE
                     RYGCSCGSGDGGETCSNRQGSGE"
     gene            complement(4534..6072)
                     /locus_tag="PKZ74_06740"
     CDS             complement(4534..6072)
                     /locus_tag="PKZ74_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92492.1"
                     /translation="MLVCITNQMNSQSYYFNDIYNPNNTWAAGLSIIGIDTGYFGCAI
                     SSDSITTNYNTCTFVMSESGKILHWNNFGRWGAHYFAGDEGALAKNNNHFGLFTSVED
                     GTLETSYGIFFKFDQYGDSILTRTFFDETDIFLIGRTCTATNDGGFALLGNNYPNDLA
                     DLLLIKTDSNGNEQWRQQYGTDSSDWAKSVIQTADKGFALGTWTRLKDGYHWNINADP
                     IVFKTDSLGNLEWTLNLGGPVMDDKAMLCPTQDSCIMVLTTIGDSMYTPSYAYAKINL
                     IKIDLAGNIIWNRNYGGSRLGNQSSSIISLENGDLLACGFVRYSIYLDPAGWLFRFNS
                     DGDSLWYREYWYFPEDPSNHGHNYLNDVSATIDNGFIAVGYAYASSPSDYLEKMWALK
                     VDSVGCEIADCWVGIEEEETGRPGDKETGRPGDKETGRGLEIWPNPARGIVDCRWSIF
                     DGRGDVVLMIYDVFGREVAEIKVPGKQDQVQINVESYPPGVYIAVLKSGGDFLGSGKF
                     VVAR"
     gene            complement(6122..>10277)
                     /locus_tag="PKZ74_06745"
     CDS             complement(6122..>10277)
                     /locus_tag="PKZ74_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92493.1"
                     /translation="IRTAYLAGSKIYRIKGLENNEPEFIDISTDVLDIPNAGTRHGNP
                     LRCEISMDKKFENMVWFFYTCEYDKGIGKSRFYRIVKFDGTAYSLIYEEGQSILAGYS
                     DFKLEFNVSPSNQSVFYAGGTSIEKIKLLPNPTSNTMKDGTYPDDNCWVHDDIRDMQI
                     FSDGTHDTLFIADNSGISWGTAFETGQTTCNYDFVWHWRHPCNSIENGLNITELYGIG
                     IFNKSSELVAGGCQDLGHMLLNTDDWINFGGGDGSEIVWDTEEMNIFYYSEWQSGSIS
                     RTNDFGATSSSINSPTFGETGLFIPLELDPINNSILYSGKKELIKFSGVNDFNGNNIT
                     VETLYTLPHDSISDIEVVRSGNSRRIYFSTLRAYYDWAPPPIEGFENCLFKSIGDGNN
                     LTFVDIGKNLDGCDGGFINDIEVKPDAISTIWVSFAVYSFDGDPKVFRTLDEGAHWED
                     YSPGLPPGLPVFKLKYDSRYGNLYAATDVGVFVRNVNSSEEWYPFNSNLPQKIVTDLE
                     INPDFNAIYAATYGRGLWKSPLHCIYNSEPFYVNDDHLIWTTNQIREQTIIIPAGKIL
                     EIRNCTVFMPSDAKIVVERGGQLILDGGTLTSACNDLWWGVELQGDSRNIQTEQYQGK
                     IIMRNEAVIEDARKAIFCGKNIENTYPDWNYTGGIIDATSSTLQNNKFGILIWRYNDQ
                     DRDYNCTLSECNFLTTKALKDGLPPRYFLTLIQVKGVTVEACDFINSVPDNIDYQDRG
                     TGIFAYDGGFTVTYISIPGQIKRSKFKKLNYGILALNYEQDQPFVVNNTYFINNLTGV
                     YASGLSEILLYDNDFTVPRAGYPTNRIFGGVYMNYCTGYTITENHYNGITPGGETNVG
                     ITVNNSGEDFNDIYKNYFTNMFIGILAQNINKSENDRQGLYILCNEFTTNEYDITVTA
                     DPWCSDLCGIARNQGTLAVPAGNLFSKTGIHDFSDYNNEKEWIDYYHHAVPLQGECPW
                     KPEHYSRQTISLHGTDETYSPCPPLDFDLEDKIATINQYNASTILIDSLEAEKTQLVD
                     GGSTIELNLEISQAQPSEAVELNDELLENSPYLSDTVLINVAGKEDVLLTEMVTDIMI
                     ANPQSAKSESVLEALESRENPLTEEMMDAILAGRDTVSEKETLEADLSHAGLQRELAL
                     NRLIDIFRQDSIDSRTDSIINLLQNHPTLSAKYRLMMIYLGQGDSIAARSTLDSIPAQ
                     FSMTSRQSIIHDHWNEWVDFILELQADSSNITDADSLQMALLLILSQNEDLPGCIANN
                     ALQFLSWTETEPHYVLPGDLLKNNPVRIKSGKEKNSETKSTIKLYPNPASGYIIVEYL
                     PNQKSYNGLLQIFTSAGRLYYAMELNNNKHFQFIDTKDWPGGIYFYKYSCENSKAENG
                     KIIIIK"
BASE COUNT         2758 a         2305 c         2219 g         2995 t
ORIGIN      
        1 gtcacagcct ttgccaacct ggtcatatcc ctgattcgga acgggatacc tccacggatc
       61 aggatcatcg tccagctgac ggttgtcgcc accctcgtca tcctggtcga ccagctcctg
      121 aaagcctatg tttacgacgt cagccggcaa ttgtcggttt ttgtcgggct gattatcacc
      181 aattgtatcc tgatgggccg cctcgaagca tttgccatgg ccaataaacc ctggccttct
      241 ttcctcgacg gtatcggtaa cgggttgggc tatagctgga tcctgattgc gctgggattt
      301 gtcagggagt tgttcggctc aggcacgctg tggggatacc cggtgctgcc gcaggcttta
      361 tatgacctgg gatacaaaaa caacggcctg atcatcctgc cgccgatggc cctcatcctg
      421 gtaggcctta tcatctggat tcaaagatct cgaacccctt cactgataga taaaagctga
      481 tttcatcatc ctaaaaccga actgtcatgc aagaatatat aaacctgttc gtcaagtcga
      541 tctttatcga caacatggtc ttcgccttct tcctgggcat gtgctcctac ctggctgtgt
      601 cgaaaacggt gaaaacttca ctgggcctgg gggtagcggt catctttgta ctgaccatta
      661 ccgttccggt caactatctc atacaaaatt atttactcgc gcctgggctc ttagctggat
      721 ttccccgtcg ctggcgggtg tagatttgaa ttttctaagt tttatgatgt ttatcgcgac
      781 gattgcttcc atggttcaac tggtcgaaat ggtggtggag aagttttcgc ccgcccttta
      841 caattctctg gggatttttc ttccgcttat tgcggtgaac tgtgcgatcc tgggcggttc
      901 attgtttatg caggagaggg aatacccgac agttgttcat gccacagttt tcggcctggg
      961 atcaggcatc ggctgggcca ttgccattat tggtattgca gcgatccgtg aaaaaatgcg
     1021 gtattccaac atccctgcac ccctcagggg actcgggata tcctttatac taaccggcct
     1081 gatggccctt gcttttatgt catttttagg tatcaaactt tagttaaatc atcttcaaga
     1141 tattgattat gaatattgga ttggtcatag gcataagtat cgtagtcttc ctggtcatta
     1201 ttcttctatt ggtcggggta ttgctttatg cgcgcgcaaa actgattccc caggggaaag
     1261 tgaagatttc aataaatgat gataaggaac tggaagtctc accgggctct tcggtgttat
     1321 ctaccctggc taacagcggg atttttcttc cttcagcctg tggcggtggc ggtacctgtg
     1381 ccatgtgcaa atgccaggta ctcgaaggcg gtggcagtat cctgcctacc gaaacagggt
     1441 atttcacacg gaaagaacaa cagaaccact ggcgcctggg ctgccaggtg aaggtcaggg
     1501 aagatatgaa actccatatc gatccgcata tcttcggaat taaaaagtgg gaatgcgagg
     1561 tcgtctccaa taaaaatgtg gccactttta tcaaagaatt cgtcgttaaa ctccccgaag
     1621 gggagaacct ggatttcaaa tcgggcggct atatccagat cgatgtcccg gtttgtgaag
     1681 tggactatgg aaaggatatt tttgtcgagg aagaataccg ggatgaatgg gataaattta
     1741 agatgtggga actgaagatg aaaaacccgg aaccggtgtt cagggcctat tccatggcca
     1801 accacccggc agaaagcaac atcatcatgc tcaacatccg catcgctact cctcctttcg
     1861 accggaaatc gggaggtttc atgaatgtta atccgggaat ttgctcctcc tatgtattct
     1921 caaggaaacc gggcgataaa gtgactatat ccggtcctta cggtgaattt ttcatcaaag
     1981 ataccaggcg cgaaatgatg ttcatcgggg ggggcgccgg aatggccccg atgcgttccc
     2041 atatttttga ccagttcaac acaaaacaca gcgaccggaa agctacattc tggtacggcg
     2101 ggcggtcgtt gcgcgaactt ttctatattg atgaattccg cgagattgaa aagaacaacc
     2161 cgaattttaa atttcatgtc gccctctcgg aaccgaaagc cgaagataat tggaccggct
     2221 ataccggctt tattcaccag gtgatcatcg ataattacct gaagcaacac cccgaaccgg
     2281 aggagattga atattacatc tgcggaccgc ccatgatgaa cgccgccatc tttaaaatgc
     2341 tcgacgatta tggtgttccc aaagaaatga tcgctttcga cgatttcgga ggataaatgt
     2401 catattgcgg cattatcttt gcgtactgaa accaaactaa aattatgaaa aaggtacttt
     2461 tatttaccct gttgtctgtt tttggcttta tgaccatttc ttttgcccag gacgtcattt
     2521 ataaaaaaga tggttccaaa gaagaggtga aagttaccct tgtcggggag aaggagatcc
     2581 aatacaagaa attcagcaat ccggacggac ctgtttacag cctgcccaaa catgagattg
     2641 tcctgataac ttatgaaaat ggtgaatttg aaaccattgt cccaaaggag tccgccggaa
     2701 tgcctgtaaa aacagatttt acggtgaatt ttgcaaagaa catcatctcc taccatatgt
     2761 tcgacctggt cttcggtgat tttacgtttt cttatgagcg gttgctgaac aacggaatgg
     2821 ttggtttcaa gattccggtg gcgatgggat attattatta cagtgatttc ggcaacttta
     2881 acagtatcat ttactccggc ctgggggtca atttctatcc gacggggcag gggagatggc
     2941 gctattttgt agggccgcag gtcagggccg ggatcggtga agaaaatgag tggatcgaat
     3001 attatgacga ttatggcaat tacctttata gtgatgacat cgagaacagg ggtttctata
     3061 cccagttttt tatcgataat ggggtaacct tcctgccggt cagaaatttc ggtatctcgg
     3121 tgattgccag catcggcgtc agatatttcc ctgaagcgtc atacgacagc gatgcgctga
     3181 gacccgacgg ccagtttgca gtgaatatca gttaccgctt ttaaaaatcc catgacaaga
     3241 ttgtcccttt ttgggttgat tctcatgcta ttatccgttt cctgctttac cccggataag
     3301 aaagtcatgc tgcggggaga agcacaggga acatattacg caattacgta ctttgaccgg
     3361 accgggagaa atttccagcc ggagatcgat tccatccttc gcagatttga tctttctgtc
     3421 tctttgtggg agcccggttc aatcctttcc aggatcaata acggcgattc tttggcatta
     3481 ccggatgaat ggttcaccga tatcttcaac cgctcagtcg atatagccga aaaaaccggc
     3541 ggagcattcg atttcaccgt cgggccgctg gtcaatgcct ggggattcgg gttcaggaac
     3601 aagatcaggc tcgattcggc aaaagtcgat agcctcctgg agttggtcga ctttaggact
     3661 gtaggactat atgaaggtaa gatagtaaaa cgggatccgc gggtgcagtt tgattttaac
     3721 gctatcgcgc aaggatatgc cgttgatgtc ctggggaatt ttctcgcttc ggagggaatt
     3781 tcgaatttcc tgatcgacat tggaggagag gtgctgggaa aagggcagaa gcccgatggc
     3841 agcgactggc tggtgggcat cgaaaatcca tccaccgaca gcctgtcgga acgtactctt
     3901 aacgcacggg tgaaactgtc gggcaaagct ttggcgactt cgggcagcta caggaaatac
     3961 tacgaggaga acggcgtccg ctattcgcac accattgatc cgaaaaccgg ttatcccgtg
     4021 agacattcgt tacttagcgt atcggtactg gccggcgatt gcgctaccgc cgacggctat
     4081 gcgacagcct tcatggtgat gggttttgaa aaggcgaaag atttcgtaga aaacgagccg
     4141 gaactggaag cttattttat taatgcctca gaagatggac tttttgagac ttatgcaaca
     4201 aaagggttca ggagtttgtt aataagtgac tgaagcaatg gttaaaaagg ttccagagtt
     4261 ccagagttcc agagttccaa atttcaaatt tcaaatttct aatttccatt ttgaatgctc
     4321 aacgtaatcc taatcacgat aatccttgtc ggtcttgcct ttgccggttt tgccattaaa
     4381 attctgctga cgaaaaaggg agagttcaaa aaaagttgtt ccagtgtcga tcctgtcact
     4441 ggtgaaagat acggttgttc ctgcgggagt ggcgatgggg gagagacgtg cagcaacagg
     4501 caaggctcgg gtgaatgagt tatgtttatg cgatcacctc gccacaacaa acttaccact
     4561 ccccaaaaaa tcgcccccac ttttcagaac cgcgatatac acccccggag gataactttc
     4621 tacattgatc tgtacctggt cctgcttgcc aggaaccttt atctcagcca cttccctccc
     4681 gaaaacatcg taaatcatca atacaacatc ccctcgacca tcgaaaatcg accatcgaca
     4741 atcgacaatc cctctcgccg gattcggcca aatctccaac cctctccccg tctccttgtc
     4801 tcccggtctc cccgtctcct tgtctcccgg tctccccgtc tcctcttctt caattcccac
     4861 ccagcaatcc gcaatctcgc acccaacgct gtctactttt agagcccaca ttttttcaag
     4921 ataatcggag ggtgatgaag cgtatgcata tccgactgct ataaatccat tatcaattgt
     4981 ggccgataca tcatttaagt aattatgacc atgattactg ggatcttcag ggaaatacca
     5041 gtactcccgg taccaaaggc tatccccatc tgaattaaaa cgaaacagcc agcctgcagg
     5101 gtctagataa atagaatacc ttacaaatcc acatgccagg agatctccat tttcaagaga
     5161 gataatactt gaactctgat ttcccaatct cgatccaccg taatttctat tccaaattat
     5221 attaccggct agatctattt tgatcagatt aattttcgca taggcataag aaggtgtgta
     5281 catcgaatct ccaatggtgg tcaaaaccat gatgcaacta tcctgcgttg ggcaaagcat
     5341 cgccttgtca tccatgaccg gtcctccaag attaagtgtc cattccaggt taccgaggct
     5401 gtcagtttta aatacgatag gatcggcatt aatattccaa tggtatccgt ctttcaatcg
     5461 tgtccatgtt cctaatgcga aacctttatc agcggtctga atcacacttt tggcccagtc
     5521 ggagctgtcg gtaccgtatt gctgccgcca ttgttcattg ccgttcgaat ctgttttgat
     5581 aaggagcaga tcggccaggt cattcggata gttatttcca agaagggcaa atcctccatc
     5641 gttagttgcc gtacaggtgc ggccaatcag aaaaatatcg gtctcatcga aaaaagttcg
     5701 tgtaagaata ctatcgccgt attgatcgaa tttaaaaaag ataccatatg atgtttctag
     5761 tgttccatct tccacagagg taaaaagacc aaaatggtta ttgtttttag ccagagctcc
     5821 ttcatcaccg gcaaaatagt gagcgcccca cctgccgaaa ttattccagt gaagtatttt
     5881 tcccgactca ctcatgacaa atgtacaggt gttataattg gtcgtaatgc tgtcggatga
     5941 gatggcacaa ccaaaatatc ccgtgtcaat tccaatgata cttaagcctg ctgcccatgt
     6001 gttatttgga ttataaatat cgttgaaata gtacgattga gaattcattt gattggtaat
     6061 gcaaacgagc ataatacaag ctaatatttt tacaatccat ttcatatcag tttatcaacc
     6121 ttcatttaat aataatgatt tttccatttt ctgctttcga attttcacat gaatatttat
     6181 aaaaatagat cccgccaggc caatcttttg tatcgatgaa ctggaaatgt ttattattat
     6241 taagttccat tgcataatat agcctgcctg ctgaagtgaa aatttgcaac aatccgttat
     6301 atgatttctg attcggtaaa tattcaacaa taatataacc ggaggcaggg ttgggatata
     6361 atttaattgt agatttagtt tcactgtttt tttcttttcc ggattttatc ctgaccggat
     6421 tatttttcaa tagatcgcca ggtaaaacat aatgaggttc tgtttcagtc cagctcaaaa
     6481 actgcaaggc attatttgca atacagccgg gtagatcttc attttgtgaa aggattagaa
     6541 gtaaggccat ttgtaaagaa tcggcgtcgg ttatattcga tgaatcagcc tggagctcaa
     6601 gtataaaatc gacccattcg ttccagtgat cgtgtattat tgactgccgg cttgtcatgg
     6661 agaattgagc aggaatactg tcgagtgtgc tgcgggcagc aatggaatca ccctggccaa
     6721 gatagatcat catcaggcgg tatttggcag aaagagtagg atgattttgc aataaattga
     6781 tgatactgtc tgtccgtgaa tcaatcgaat cctgcctgaa aatatcaatc agcctgttta
     6841 acgccagttc ccgctgtaat ccggcatgtg aaagatccgc ttcaagggtt tccttttcgg
     6901 aaaccgtgtc tctgcctgct agtattgcat ccatcatctc ttcggtcaat ggattttccc
     6961 ggctttccag cgcttcaagc acgctttcgg atttggcgga ttggggattg gcgatcatga
     7021 tatccgtaac catttcagta agcaaaacat cttctttacc ggccacatta attaataccg
     7081 tgtcggaaag atacggagaa ttttccaata attcatcatt caattcaacc gcttcagatg
     7141 gttgtgcctg ggaaatttcc agatttaact cgattgtaga tcctccgtcg accaattgag
     7201 ttttctcagc ttctaatgaa tcaattaaaa tagtcgaagc attatactga ttaatagttg
     7261 ctattttatc ctccaagtca aaatccagtg gaggacaagg agagtaggtt tcgtctgtcc
     7321 cgtgtaagct tattgtctgt cttgaataat gttccggttt ccagggacat tctccttgta
     7381 agggtactgc atgatgataa taatctatcc attccttttc attattataa tcgctgaaat
     7441 cgtgaatacc ggttttactg aacaggttac cggctggtac agccagggtg ccttggtttc
     7501 gtgcgattcc gcataaatct gagcaccagg gatcagctgt tacagtaata tcgtattcat
     7561 ttgtggtgaa ttcattgcaa agaatatata aaccttgtct atcattctcg cttttattga
     7621 tattttgtgc taagatgcca ataaacatgt tggtaaaata attcttgtaa atatcattaa
     7681 aatcctcccc cgaattattg accgtgattc caacattggt ttctccaccg ggggttattc
     7741 cattataatg gttttccgta atagtataac cggtacaata attcatataa acacctccga
     7801 aaatcctgtt tgtgggatat ccggctctcg gtacagtaaa gtcgttatca taaaggagaa
     7861 tctcactcaa cccgctggca tagacgcccg tgaggttatt gataaaatat gtgttgttaa
     7921 caacaaatgg ctgatcctgc tcatagttca gtgccagtat cccataattg agctttttaa
     7981 atttggacct ctttatttgt ccgggaattg aaatataagt cactgtgaat cccccgtcgt
     8041 aagcaaagat tcccgtcccc ctgtcctgat aatcaatgtt gtcaggtact gaatttataa
     8101 aatcacatgc ttccacagtg acacctttaa cctgaataag tgtaaggaaa taccttgggg
     8161 gcaaaccatc ttttaaagcc ttagtggtaa ggaaattgca ttccgagagg gtgcagttat
     8221 agtcccggtc ctgatcattg tatcgccaga tcaggatccc gaatttgtta ttttgaagag
     8281 tcgaactggt ggcatcaata attcctccgg tatagttcca gtcggggtaa gtgttttcga
     8341 tgtttttgcc gcaaaatatg gcttttcggg catcttcgat aaccgcttca ttcctcatga
     8401 ttattttccc ttgatattgc tcagtctgaa tattccggga atcgccctgc aattcaacac
     8461 cccaccaaag gtcgttgcag gcgctggtaa gcgttccgcc gtcaagaatg agctgtcctc
     8521 ctctttccac aacgattttc gcatcggaag gcataaaaac agtacaattg cgaatctcga
     8581 ggattttgcc tgccgggatt atgatagtct gctccctgat ttgatttgtc gtccagatca
     8641 aatggtcatc gtttacataa aatggttcgc tattgtatat gcaatgaaga ggcgatttcc
     8701 ataaaccgcg tccgtacgtg gcagcataaa tcgcattaaa gtcgggattg atctccaggt
     8761 cggttacgat cttttgaggc agattagagt tgaatggata ccattcctca ctgctgttga
     8821 catttctgac aaatacccct acatccgttg cggcataaag attaccatat cttgagtcgt
     8881 atttaagctt gaatacaggc aaaccgggcg gtaatcccgg agaataatcc tcccaatgcg
     8941 ctccctcatc aagcgttcta aatacttttg gatctccatc gaacgaatag acggcaaaag
     9001 atacccaaat ggtagagatc gcatcaggtt tcacttcaat atcattgatg aaaccaccat
     9061 cacagccgtc taaatttttc ccaatatcta caaaagtgag attatttcca tcgcctattg
     9121 atttgaaaag gcagttttca aaaccctcaa ttggaggagg agcccagtcg taataagctc
     9181 tcagtgttga aaagtaaatt ctccgggaat ttccggatct gacaacttca atatctgaaa
     9241 ttgaatcgtg aggcagggta tacagagttt ctactgttat attatttcca ttaaaatcat
     9301 tgacaccgga aaactttatt aattcttttt taccggaata aaggatcgag ttgtttatcg
     9361 gatcaagttc caggggtata aataggccgg tttctccaaa ggtcgggcta ttaatagaag
     9421 atgatgttgc gccgaaatca ttagttctag aaatcgagcc tgattgccat tcgctatagt
     9481 agaaaatatt catttcttca gtatcccaaa ctatttcact tccatcccct ccgccaaaat
     9541 tgatccagtc atcagtattt agaagcatat gtcccaggtc ttgacagcca cccgcaacca
     9601 attcggaaga tttattaaat atccctattc catatagctc tgtaatattc aaaccatttt
     9661 caatagaatt acatggatga cgccaatgcc atacaaaatc gtaattacat gttgtctgcc
     9721 cggtttcaaa cgccgttccc cagcttatcc ccgaattatc tgcaatgaat aacgtatcat
     9781 gggttccatc gctgaagatt tgcatatccc ggatatcatc atgaacccag caattgtcgt
     9841 caggatatgt accgtctttc atagtgttgc ttgtgggatt gggtaagagt ttgatttttt
     9901 caatacttgt gcccccggca taaaaaacac tttggtttga aggtgataca ttaaactcga
     9961 gtttgaagtc ggaataacct gctaaaatgc tttgcccctc ttcatagatt aaactatatg
    10021 cagttccatc aaacttgaca attctataaa acctgctttt cccaattcct ttatcatact
    10081 cacatgtgta aaaaaaccaa accatatttt caaacttctt atccatactg atctcgcacc
    10141 gaagaggatt tccgtgcctt gtccctgcat tcggaatatc aagtacatct gtagaaatat
    10201 caataaactc cggctcgttg ttttcaaggc cctttatcct atagattttg ctgccggcaa
    10261 gatatgctgt tctaatc
//
LOCUS       DAOPNH010000101        10212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7102_length_10212_cov_8.702767, whole genome shotgun sequence.
ACCESSION   DAOPNH010000101 DAOPNH010000000
VERSION     DAOPNH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7102_length_10212_cov_8.702767"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..460
                     /locus_tag="PKZ74_06750"
     CDS             89..460
                     /locus_tag="PKZ74_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPM92494.1"
                     /translation="MEFDDLSRKIIGCALQVHKILGPGMLESAYEECLAYELTSTGLM
                     IHRQKPIPIIYKDIKLDCGYRLDLLVEQKIIIELKSVELLAPVHEAQILTYMRFAEIR
                     LGLLINFNVTILKSGIKRYVL"
     gene            complement(592..792)
                     /gene="xseB"
                     /locus_tag="PKZ74_06755"
     CDS             complement(592..792)
                     /gene="xseB"
                     /locus_tag="PKZ74_06755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPM92495.1"
                     /translation="MEKQITYTEAMKELELIVGEIEEGEITIDSLSEKVKRASELIRI
                     CKEKLTATEEDVSKILEGLKET"
     gene            complement(797..2191)
                     /gene="xseA"
                     /locus_tag="PKZ74_06760"
     CDS             complement(797..2191)
                     /gene="xseA"
                     /locus_tag="PKZ74_06760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPM92496.1"
                     /translation="MTGNHHNGAEIMTLSQLAAGITVVLEENFRSPLWIKAEIAKLNY
                     YPASGHCYPSLVEKEKGSVKAELRGTIWANDYVRINSNFLKVIREPLKEGIQILFRAY
                     VRFHAVYGLSLQIIDIDSSYTLGEMAREKMETLARLKGEGLFDRNRQLELPALPKRIA
                     VISVKSSKGFSDFINIIENNPWKYGISCVLFPALLQGDKAVESIRSQMELIGRYAHLF
                     DAVAIIRGGGGEVGLNCYDNYMLARDVALSQLPVLTGIGHSTNETVVEMIAGYNKITP
                     TDVAYFIIQQFHNFSGRLNELHENLATIANDILEEEKKRWEVFCLELATQTENLVRNE
                     KRAILELSGSVIDGTGILITGQKHLLNLAGLKLSYKPAGLLRESRTGLENHWERFFLH
                     VKQLLKQKNSEIENLNHKINLLNPVHVLKRGYSITYLNGKALIDCSEAKAGDNLTTRL
                     YKGEISSEVKSLKD"
     gene            2382..3134
                     /locus_tag="PKZ74_06765"
     CDS             2382..3134
                     /locus_tag="PKZ74_06765"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPM92497.1"
                     /translation="MENNTQPAVRHGLKGKSLVFGVLVILLGIALFFRNLEPFEGARI
                     WDVIFSWQMLLIAIGLVNIVNDSSRRVGWILIAIGGFFMIPVIFDIENYRSTFWPALL
                     IIIGLILIFGSSRLCKRSYSHNKTWTDSSGFSSGTITDDFVEEVAVFGGRERFINSQS
                     LKGAKVVSVFGGSTLDMTKVELAPGNVEIEIVAIFGGSTLVVPNDWNIKLEVFNIFGG
                     YSDKRIRGQVDFNKTVIVKGVAIFGGGEIKSY"
     gene            3154..4209
                     /locus_tag="PKZ74_06770"
     CDS             3154..4209
                     /locus_tag="PKZ74_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM92498.1"
                     /translation="MARGIKNHLEIVYLIMWVTLGVIHFSLLYFGYGLRSVHAFADSL
                     VFNGLFTVIGSGLWFMVRYSNLQTKTKAELALYHITGAAVAIFVWMMAGHLILNTLFP
                     EDQAYQSFLKNTVFLRILAGLMFYALLVSIFYLLINFRELKEKSQREARLTGLLKEAE
                     LNLLRSQIRPHFLFNSLNSISALTMTDPGKAQEMVIKLSEFMRYSLNFPDNMTGTLER
                     ELYNAELYLDIEKVRFGSRLAFEKEIQPEAMSWIVPVMILQPLVENAVKYGVYESEET
                     TVIRLGARILESELEIRIGNTYGFGTMAKKGTGTGLRNIRGRLLNLYGTGSLMKINQT
                     ENYFEVTIRIPEYARKD"
     gene            4193..4948
                     /locus_tag="PKZ74_06775"
     CDS             4193..4948
                     /locus_tag="PKZ74_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM92499.1"
                     /translation="MQEKIKVIIVDDEDLARELVKKYLAGLADAELLCECSNGFEALK
                     AIQELRPDLVFLDIQMPKIDGFELLEVLDEKPAIIFTTAFDQYAIRAFELNAVDYLLK
                     PFSRERFVQAYEKAKLRLGTTPQGMASQGTPSLLKHLDDTRQTLDRVITRLGSKITVI
                     PVDKIRYIESADDYVMIHSELGDHLKEKTMKYFEEHLPKEQYIRIHRKYIVNINEIRS
                     LELYTKDSYVALLKTGEKLNVSSEGYRRLRERF"
     gene            complement(5031..6320)
                     /locus_tag="PKZ74_06780"
     CDS             complement(5031..6320)
                     /locus_tag="PKZ74_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92500.1"
                     /translation="MSSKNVIIKICQKKIWFNETVSIAVECSNLPVDDIKFSRVNEIY
                     WLTEQVSFEKDTGLLEVNIQDYFLRDFSSFTAQNPKSLVKSIRFRNLSDTDSLKTALR
                     YYSSGMNHLDQAMEEAFRELSDKPEPIPEKVWKKNIMFRSYQEKEKLEVTFDAWFKDA
                     EFKLGYVAFDRNIPELKQMLRFKVDNDFLLPEFEFIKSYFVKALGTRKFDVKATIFLE
                     HGKPVETSAASVLIKKIDENLIDSIKNIRTAALFKPPFKVNVDKSLFSADEVFGEFGP
                     GEAGNVFSQTEEEMLGQILEKYKVRNRKQLEFLAGVKQAAGSRLKFTLNPYFGFLFTV
                     EGEKMNHFIWELLNSHATYIWSLDKGDGAMARQYRRVEDAINQVRNSGRDNYKQAWRA
                     SHIDHDLVFHVIHHDDAGSAMIDGFVKWRHRLGEVMI"
     gene            6457..7248
                     /locus_tag="PKZ74_06785"
     CDS             6457..7248
                     /locus_tag="PKZ74_06785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HPM92501.1"
                     /translation="MNFLYIISAAFVLVVLVLLVRYAWGIFFDSNYRPVEWEYAVING
                     RVSKELEKLERNYPDKVRFFNWWFQVERLKRDGIPGDFAELGVYKGESARIIHMMDPS
                     RTFHLYDTFEGFKEEDLENETGEAATYTTRNFADTSLEAVKRYLGEEGKFVYHAGRFD
                     FSAPTPSLPPAGGEGEREGAYALVNLDADLYNPTKAGLEYFYPRLSPGGVIFIHDYTH
                     KWPGIKRATDEFISKIPETLVHVPDMDGTVMIVKISSPALQLPRL"
     gene            complement(7214..7621)
                     /locus_tag="PKZ74_06790"
     CDS             complement(7214..7621)
                     /locus_tag="PKZ74_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPM92502.1"
                     /translation="MKKIAALFVLVLVGVQIISAQTQAVQQQPAKTYSWNETTHDFGK
                     IVVNDPATVTFTVKNNGTAPLLMTAAKSSCGCTVAEYTKEPIKPGETGIVKATYNAAR
                     AGSFTKTVTVTFEGISSGELLTIKGEVIAKPEN"
     gene            7775..9043
                     /locus_tag="PKZ74_06795"
     CDS             7775..9043
                     /locus_tag="PKZ74_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM92503.1"
                     /translation="MTNRSVQWVIGLGILAILAILAGQAYFFYQDFNLRERQTVQSFR
                     ISLQSVAESISQFNKSTLPDNIIHQYSPDYFIVDINNRIDPWVLEHYLRTELMRRHLD
                     VDFEYAIYDCDDDRMVYGNYVSLDQTEEVKNKLEYWPKYEEGLYYFGVHFPGMRNYLL
                     GEMGLWYFFTGILVMVILFFGYSMMIILRQKKLSEIQRDFINNMSHEFRTPLTSIGLA
                     SDVILEEGQEGDKARFSKYSAILKDQIIQLQKKVDKILVHAETENKFFRLNTQKLYPE
                     DVVTEVLEELKPSVEHSNGVVRFTVQSGNQLINADRYHFSGILINLIDNSLKYTSKPP
                     DVCITLSANPKRVFIKLEDKGTGIDSKYLRKVFMPFFRVPSGNIHNVKGTGLGLSYVK
                     QVCDLHGWKVKIESAPGQGTTVTISIPKTE"
     gene            9045..9737
                     /locus_tag="PKZ74_06800"
     CDS             9045..9737
                     /locus_tag="PKZ74_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92504.1"
                     /translation="MVTRSRILYVEDDETLGFITSENLERKGYEVILCKDGESAYRLF
                     KTESFDLCLFDVMLPKLDGFTLARLVRKENEEIPIIFISAKTLTEDKIEGLLLGADDY
                     LVKPFNIEELTLKIEVFLRRSRVNKAESPESEIRIGSCTLDMGLLRLECGGIVKKLTF
                     REAELISYFSRNRNKLLSREQILEAVWGGNDYFSGRSLDVFISRLRKYFQDDKLVSIE
                     NRHGLGYVFEVK"
     gene            complement(9782..>10212)
                     /locus_tag="PKZ74_06805"
     CDS             complement(9782..>10212)
                     /locus_tag="PKZ74_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM92505.1"
                     /translation="ALEADALHLKTDVYTSLGVGIGLLLIWITKIHIIDPIIAILVAV
                     IILKEAYELLARAFSPLLDTSIKLADLKKLEDSLVKMGVKYHDLKTRKAGSYIFVEFH
                     LEMPPDKKLEEVHAYCDRIEEELKKQISNLRITIHAEPEE"
BASE COUNT         2590 a         2406 c         2472 g         2744 t
ORIGIN      
        1 cccctttgtt gcaagcataa caatatttgt ttgaataaaa aaacaaacat ataagaaaac
       61 acgaagttac acgaagaaaa tgttgcaaat ggaatttgat gatttatccc gcaagataat
      121 aggctgtgct ttgcaggtac ataaaatcct tggtccggga atgctggaat cagcatatga
      181 agaatgcctc gcttacgagt tgacctcaac tggactaatg atccacagac aaaaaccaat
      241 tcctataatt tataaggata ttaaacttga ttgtggttat cgacttgatt tgcttgttga
      301 gcagaagata atcattgaat taaaatcagt agaattatta gcgcctgtcc atgaagccca
      361 gattctgact tatatgaggt ttgcagaaat caggttagga ttattgatca actttaatgt
      421 gacaattcta aagagtggta taaaaagata tgttttataa ccttcgtgat ccttcgtgac
      481 tccttcgtgg atcttcgtga aaataaatat atatgattct aatacaataa gttacacgaa
      541 gaaaacacga agttatgcga ataaatcaag acctaaaaat aggggtcatc atcatgtttc
      601 cttcaatcct tccagaatct tgctgacatc ctcctccgtc gccgtcagct tctccttgca
      661 aatcctgatc agctccgatg cccgctttac tttttcggaa agcgaatcga tcgtgatctc
      721 cccttcttct atttcaccga cgatcagttc aagttctttc atcgcctcgg tataagtaat
      781 ttgcttctcc atttgattaa tcctttaatg atttgacttc actcgaaatt tcccctttat
      841 aaagccgggt tgttaagtta tcccccgctt ttgcctctga acagtcaatt agtgcttttc
      901 cgttgaggta tgtgatgcta taacctcttt tcagcacatg gaccggattc agaaggttta
      961 ttttgtggtt taggttttca atttcgctgt ttttttgctt caacagttgt ttcacatgca
     1021 gaaaaaaacg ctcccaatgg ttttcgagac cggtcctgct ctcccgcaac agtcctgccg
     1081 gtttgtacga cagtttcaat ccggccaggt tgaggagatg tttctggccg gtaatcaaga
     1141 tccccgttcc atcgatcacc gaccccgata actccaatat cgcccgcttt tcatttctga
     1201 cgagattttc tgtttgcgtg gctagctcca ggcagaatac ttcccacctc tttttttcct
     1261 cttccaaaat atcatttgca attgttgcca gattctcatg gagttcgttc aaccttcccg
     1321 aaaaattatg gaactgctga ataatgaaat aagcgacatc ggtgggggtg atcttgttgt
     1381 aaccggcgat catttccact acggtttcat tggtggaatg cccgattccg gtcagcaccg
     1441 gcagctggga aagagctaca tccctggcca gcatgtagtt gtcgtagcaa ttcagcccca
     1501 cctcacctcc accgcccctg ataatggcaa ccgcatcgaa caggtgtgcg taccggccga
     1561 taagttccat ctggctccgg attgactcta ccgctttgtc cccctggagc agcgcgggga
     1621 agaggacaca actgatccca tatttccatg ggttattttc gatgatattt atgaaatcgc
     1681 tgaagccctt gcttgatttt accgaaataa cggcaattct cttcggcaga gcgggtaatt
     1741 caagctgcct gttccggtcg aacaggcctt cgcctttgag ccgggccagg gtctccatct
     1801 tttcccgggc catctcgccc agggtatacg acgagtcaat gtcgatgatc tggagagaaa
     1861 gcccgtacac agcatgaaac ctgacatagg cccggaagag tatctgtata ccttccttca
     1921 gcggttcgcg tattactttc aggaaattac tattgatcct tacatagtcg ttagcccaga
     1981 tcgtccccct cagttcggcc tttacgctcc ccttttcctt ttccaccagt gaaggatagc
     2041 aatgaccgct ggccgggtag taattcagct tggctatctc ggctttgatc cataacggcg
     2101 accggaagtt ttcttccaaa accaccgtaa tgcctgccgc gagctgcgac aaggtcatga
     2161 tttccgctcc gttatgatga tttcctgtca ttcaatcttc aacctgcaaa cttcaaccta
     2221 caatcttcaa ccttcaatgc tcaaaagtac cactattttc ccttgtttac cgattccggc
     2281 ccggtcttcg ccgatttcag gttctctggc gattgtcgcc gtattatttt tgccccatca
     2341 aatttgtgaa aacaatgttc aacattcaaa atcatacgaa aatggaaaac aacacacaac
     2401 ctgccgtaag gcatggcctt aaaggcaaat cactggtatt cggcgtcctg gtcattctac
     2461 tcgggatcgc tctttttttc agaaatctgg aaccctttga aggcgcccgg atctgggacg
     2521 ttattttctc ctggcagatg ttgctgatcg ccatcggcct ggtgaatatc gtgaacgact
     2581 cctcccgccg cgtaggctgg atcctgatcg cgatcggcgg attcttcatg atcccggtca
     2641 tctttgatat tgaaaattac cggagcactt tttggcccgc cctgttgatc attatcgggc
     2701 tgatccttat tttcggctca tccaggctct gcaaaaggtc ctattcacat aacaaaacct
     2761 ggaccgattc ttccgggttt tcctccggca ccatcacaga tgacttcgta gaagaggtag
     2821 ccgttttcgg cggccgggag cggttcatca attcgcaatc gctcaaaggg gccaaggtgg
     2881 tctcggtttt cggaggatcc accctcgaca tgaccaaagt ggagctggca cccggcaacg
     2941 tggagattga gattgtggct attttcggcg gatcgacgct ggttgttccg aacgactgga
     3001 acatcaaact ggaagttttt aatatctttg gtggatacag cgataagcgg atcagggggc
     3061 aggtcgattt caacaagacg gttatcgtaa aaggggtagc catttttggc ggaggggaaa
     3121 taaagagcta ctgatcccgg atgctggatt gatatggcaa gaggaataaa aaatcacctg
     3181 gagattgttt acctgatcat gtgggtgacg ctgggcgtca tccacttttc ccttttgtat
     3241 ttcggttatg gcctccggtc ggtccatgct tttgccgaca gcctggtttt caacgggctt
     3301 tttacggtga tcggcagcgg gctgtggttt atggtcaggt actccaattt gcagacaaaa
     3361 accaaagccg agctggcgtt ataccatata accggcgcag ctgtggccat ttttgtatgg
     3421 atgatggcgg gccacctgat tctgaataca ttattccctg aggatcaggc ctatcaatct
     3481 tttctaaaga ataccgtctt tttgaggata ttggcgggat tgatgtttta cgccctgctg
     3541 gtttccattt tttacctgct gatcaatttc agggagttaa aagaaaagag ccagcgggaa
     3601 gcaaggctca ctggattgct gaaagaggcg gagctgaatc ttctgcggtc gcagatcagg
     3661 cctcattttt tatttaacag cctgaactcc atcagcgccc tcacgatgac cgatccggga
     3721 aaggcacagg aaatggttat caagttatcc gagtttatgc gctattcgct gaactttccg
     3781 gacaatatga ccggtacgct ggaaagggag ctctacaatg ccgaacttta cctggatatc
     3841 gagaaggtcc gtttcggcag ccgcctggca tttgagaagg aaatacagcc cgaagcgatg
     3901 agctggattg taccggtcat gatcctgcaa ccactggttg aaaacgccgt caaatatggt
     3961 gtttacgaat ctgaagaaac gactgtaatc cggctggggg cccggatcct ggaaagcgag
     4021 ctggagatca ggattggcaa tacctacggt ttcggtacca tggcaaaaaa ggggacgggg
     4081 accgggttgc gcaacatccg gggcaggtta ctgaatcttt atggaaccgg cagcctgatg
     4141 aagatcaatc aaacggagaa ttattttgaa gtcactatca gaattccgga atatgcaaga
     4201 aaagattaaa gttatcattg tggatgatga ggatctggcg cgggaattgg taaagaaata
     4261 cctggccggg ttagccgatg cggagctgct ttgtgaatgc tccaacggtt tcgaggccct
     4321 gaaggcgata caggaactca ggcctgatct tgtgttcctg gatatccaga tgccgaagat
     4381 tgatggtttt gagttactcg aggtgctgga tgagaagccg gccatcatat tcactacggc
     4441 ttttgaccag tatgctatcc gggcgttcga gctgaatgcg gtggattatc tgctaaagcc
     4501 attttcgagg gagcgtttcg tgcaggcgta tgaaaaggcg aagctccggt tagggacgac
     4561 accccagggg atggcgtccc aagggacgcc atcccttttg aagcacctcg acgacacccg
     4621 gcagaccctc gaccgggtca tcacccggct cggctccaaa attaccgtta taccggtgga
     4681 taagatcagg tacatcgaat cggccgatga ttacgtcatg atccattccg agctgggcga
     4741 ccatctgaaa gagaagacca tgaagtactt cgaggagcac ctgccgaaag agcagtatat
     4801 ccgcatccac cgcaaataca ttgtcaatat caacgaaatc cggtcgctgg aactttacac
     4861 aaaagacagc tatgtcgctc tgcttaaaac cggcgagaaa ctgaacgtga gttcggaagg
     4921 gtacaggcgg ctgagggaga ggttttagat tgggcgattg ggcgattggg cgattaaccg
     4981 ctacgcggta aaaacatcaa attcgtcaaa tccaccaatc ccccaattcc ttaaatcatc
     5041 acctcgccca gccggtgccg ccattttaca aacccatcaa tcatagccga gccggcatcg
     5101 tcgtggtgga tgacatggaa caccaggtca tggtcgatgt gggaggcgcg ccaggcctgc
     5161 ttgtagttgt cgcgcccgct gttgcgcacc tggtttatgg cgtcttccac ccgccggtac
     5221 tgcctggcca tcgcgccgtc gcccttgtcg agactccaga tgtaagtagc atgggagttc
     5281 agcagctccc agatgaagtg gttcattttc tccccttcaa cagtgaatag aaacccgaaa
     5341 tagggattca gcgtgaactt cagcctcgat ccggccgcct gctttacgcc cgccaggaat
     5401 tcgagctgct tgcggttccg aaccttgtac ttctccagga tctgcccgag catctcctct
     5461 tcggtctggc tgaaaacatt gccggcttca ccggggccaa actcaccgaa aacctcatca
     5521 gccgaaaaaa ggcttttgtc gacattgacc ttgaagggag gtttgaatag agcagccgtc
     5581 cggatattct tgatgctgtc gatcaggttc tcatcgattt tcttgatcag gaccgatgca
     5641 gcgctggttt cgaccggttt cccgtgttcc aggaatatag tggctttgac gtcgaatttc
     5701 cgggtcccga gcgctttcac gaagtagctt ttgatgaact caaactcagg caggagaaaa
     5761 tcattatcaa ccttaaaccg cagcatttgc tttagttccg ggatgttcct gtcgaaagcc
     5821 acatagccta gcttgaattc ggcatccttg aaccaggcat cgaaagtcac ttccagcttc
     5881 tctttttcct ggtacgaccg aaacatgata tttttcttcc agactttctc cgggatgggt
     5941 tccggcttgt cggaaagctc ccggaaagcc tcttccatcg cctggtcgag gtggttcatt
     6001 ccgctcgaat agtagcgtaa tgccgtcttc aggctgtctg tatcgctgag gttccggaac
     6061 cggattgatt taaccagcga ttttggattt tgagcggtaa aagaggaaaa gtccctgaga
     6121 aaataatcct ggatattcac ttcgagcagg ccggtatcct tttcaaaaga gacttgctct
     6181 gtaagccagt agatctcatt cacccggcta aatttgatat catcgacagg caggtttgaa
     6241 cactcaaccg cgatcgatac ggtttcattg aaccatatct ttttctggca gattttaata
     6301 atgacgtttt tactgctcat acccgctcac aggaaataat agcactttta aatccagacc
     6361 gaaattagaa aattaattcc gaattcttca aatcaaccca tccgcccact aactttgcac
     6421 tctgaacttt gagttttgca ctttgaactt tgcactttga actttcttta cattatttcc
     6481 gccgcttttg tcctggtcgt acttgttctc ctggtaaggt atgcctgggg gattttcttt
     6541 gattcgaact accgtccggt ggagtgggaa tatgccgtta taaatgggcg ggtcagcaag
     6601 gaactcgaaa agctggagcg taattacccc gataaggtcc gttttttcaa ctggtggttc
     6661 caggtggagc ggctgaaaag ggatggcatc cccggcgatt tcgcggaact gggcgtttac
     6721 aaaggggaaa gcgcaaggat catccacatg atggacccgt cacggacttt tcatctctac
     6781 gatacttttg aaggctttaa agaagaagat cttgagaacg aaacgggaga ggcggcaacc
     6841 tatacgaccc gtaatttcgc cgacaccagc ctggaagcgg tgaagcgtta cctgggggag
     6901 gaagggaagt ttgtgtatca tgcggggagg tttgattttt cagcccccac cccgtccctt
     6961 ccgccagctg gcggagaggg agaaagggag ggggcttacg ccctcgtcaa cctcgatgcc
     7021 gacctgtaca acccgaccaa agccggactg gagtattttt atccccggtt gtcgcccggt
     7081 ggtgtcattt tcatccacga ttatacgcac aaatggcccg ggattaagcg ggccacagat
     7141 gaatttatca gcaaaattcc ggaaacgcta gtccatgtcc cggatatgga tggaacggtg
     7201 atgatcgtga agatcagttc tccggctttg caattacctc gcctttgatg gtgaggagtt
     7261 caccggaaga gattccttca aaagtaacgg tcaccgtttt ggtaaaggag ccggccctgg
     7321 ccgcgttata ggtagctttc acaatacccg tttcacccgg tttgatcggt tccttcgtgt
     7381 attcagccac ggtacagcca cacgagcttt tagccgcggt catgagcaag ggtgccgttc
     7441 cgttgttttt taccgtaaaa gttaccgtag ccggatcgtt cacgacaatc ttcccgaaat
     7501 catgggtcgt ctcattccat gaataggttt tggcaggctg ctgctgcact gcctgggtct
     7561 gtgctgaaat gatctgcaca cccactaata ccagaacaaa taatgctgca atttttttca
     7621 tttgattata tttaaattta tttgaagaat cttcggccaa aattagggga acagaccgga
     7681 aggattggtt aacaaagtca taattattct taatgaatag taaatgaatt gttaatagaa
     7741 ataccatcag cctcaatttt agtaatttcg ctttatgaca aaccgttcgg tacaatgggt
     7801 aatcggcctg ggtatcctgg ccattttagc catcctggcc gggcaggcct atttttttta
     7861 ccaggatttt aatctgcgtg aacgacaaac agtccaatcc ttccggattt cattgcaatc
     7921 ggtggcggaa agcataagcc agttcaataa aagcactttg cctgacaata tcatccatca
     7981 gtacagcccc gattatttta ttgtcgatat caacaacagg atcgatccct gggtactgga
     8041 acattattta cgcacagagc tgatgcgccg ccacctcgat gtcgatttcg aatatgccat
     8101 ctatgattgt gacgacgacc gcatggttta tggaaattac gtttcgctcg atcagacgga
     8161 ggaggtgaag aacaagttgg aatactggcc aaagtatgag gaagggctct attattttgg
     8221 ggttcatttt ccgggaatgc gaaactacct tctgggggag atgggattat ggtatttttt
     8281 tacaggtatt ctggtcatgg tcatattatt tttcggttac agtatgatga tcatccttcg
     8341 ccagaagaag ctttcggaga ttcagcggga ttttatcaac aacatgagcc acgaattccg
     8401 tacccccctc acttccatcg gcctcgcctc ggatgtcatc ctggaagagg ggcaagaggg
     8461 cgataaagcc cggttcagta agtattcggc gatcttaaaa gaccagatca ttcagcttca
     8521 gaaaaaggtc gataaaatcc tggtccatgc cgaaacggaa aacaaatttt tccggctgaa
     8581 cacccagaag ttatacccgg aggacgtggt taccgaagtg cttgaagaac tgaagccatc
     8641 ggttgagcat tcgaacggtg tcgtgagatt caccgttcaa tcgggaaatc aactgatcaa
     8701 tgccgatcgt taccactttt caggaattct tatcaatctc atcgacaatt ctttaaaata
     8761 caccagcaag cctccggatg tttgcattac cttgtcggcc aatccaaagc gggtatttat
     8821 taaattggaa gataagggaa ccggtattga cagtaaatat ctcaggaagg tattcatgcc
     8881 cttttttcgg gtccccagcg ggaacattca caatgtcaag gggaccggcc tggggctgag
     8941 ttatgtcaag caggtgtgcg atctgcatgg ctggaaagtg aaaattgaga gtgcgccggg
     9001 gcagggaacc acggtgacga tttccatacc caaaacagaa tagtatggta accagaagcc
     9061 ggatattgta tgtcgaagac gacgaaacgc tcgggttcat cacctccgag aacctggaga
     9121 gaaaggggta tgaggtcatt ttgtgcaaag acggagaatc ggcatatcgt ctcttcaaaa
     9181 cagaatcctt cgatttatgc ctgtttgatg tcatgctccc aaaactcgac gggtttacgc
     9241 tggcacgcct tgtcaggaag gaaaacgagg agatcccgat cattttcatt tcagctaaaa
     9301 cactgacgga agacaagata gaagggctgt tgctgggcgc cgacgattac ctggtgaagc
     9361 catttaacat tgaagagtta acattgaaga ttgaggtgtt cctgaggagg tcgagggtaa
     9421 ataaagctga atcacctgaa agtgaaatcc ggatcggcag ttgcaccctg gacatgggcc
     9481 tgttgcggct ggaatgtgga ggaattgtga aaaaactgac attccgcgaa gctgaactga
     9541 tcagttactt cagccggaac cggaataaat tgctcagccg ggagcagatc ctggaggcgg
     9601 tatggggtgg taacgactat ttctccggcc ggagtctcga tgtattcata tcgcggttac
     9661 gaaaatattt ccaggatgat aagctggtca gcatcgaaaa ccggcacgga ctggggtacg
     9721 tttttgaggt gaaataaaag cgaaaaaagg gacgccatcc tttgggacgc catccctggg
     9781 atcattcctc cggttcggca tgtatggtaa tacgcaggtt gctgatctgt tttttcagct
     9841 cctcttcaat cctgtcgcag taagcgtgca cttcttccag tttcttgtcg ggcggcatct
     9901 ccaggtgaaa ttccacgaaa atatagctgc ctgctttccg ggttttcaga tcgtgatatt
     9961 ttacacccat ttttaccagc gaatcttcca gttttttaag atcagccagt ttaatcgaag
    10021 tatcaagcaa tggggaaaag gccctggcta ataattcata ggcttctttc agaataataa
    10081 cagccaccag gatggctatg atggggtcga tgatatggat cttggtgatc catataagca
    10141 gcaatccgat tccgactcca agggaagtgt aaacatcggt cttgaggtgc agggcgtctg
    10201 cttcaagggc ca
//
LOCUS       DAOPNH010000102        10168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7141_length_10168_cov_6.569959, whole genome shotgun sequence.
ACCESSION   DAOPNH010000102 DAOPNH010000000
VERSION     DAOPNH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_10168_cov_6.569959"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..153
                     /locus_tag="PKZ74_06810"
     CDS             13..153
                     /locus_tag="PKZ74_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPM92506.1"
                     /translation="MLLAGPSLSLPNMLVIRGVMGTQKTVMYVVLVVIMATISGLIYG
                     TI"
     gene            164..439
                     /locus_tag="PKZ74_06815"
     CDS             164..439
                     /locus_tag="PKZ74_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM92507.1"
                     /translation="MAIQIVILCPGSKCKKCRRMIRQVEEAVLESGLEAEVKILDTLD
                     DLMKYNTYVLPALVINGKMLARGYVPEKSMIIKEMNKLPDNGKKYES"
     gene            429..842
                     /locus_tag="PKZ74_06820"
     CDS             429..842
                     /locus_tag="PKZ74_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPM92508.1"
                     /translation="MSLEIEKYGFVINDLRHVSGKECLEILKTDGFILDVRPDFELTR
                     LIDHEHILYYPYDEIREHFRKLPHDTWLILADAVGLRSKEVAFFLKENGFSNILHLAG
                     GVVEWERDGLPVTLDKSRRLTGSCMCQLKRREIKK"
     gene            895..1308
                     /locus_tag="PKZ74_06825"
     CDS             895..1308
                     /locus_tag="PKZ74_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92509.1"
                     /translation="MKKHITFMMAVMLTISAGMIFGQSNKTSTEVLYFKANLCKCKAG
                     ACNALQADVDSVIVKYFQKENIEFKVIKLADEANKELVSRFNAKSQTVVIISKKKKKE
                     NVIDASAIVADYSKTKDKVRFEAEMRKKISESLVF"
     gene            1340..1714
                     /locus_tag="PKZ74_06830"
     CDS             1340..1714
                     /locus_tag="PKZ74_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92510.1"
                     /translation="MSESNKPGFIMCVCTGKCPGFSQMDIWDFINRVRIELPVEYGFI
                     HPQLCEEDGDRFLLDFLKAHRKVVIGGCAPNMQMKLFRDAFKESGLEISTDLIPIDVR
                     DMTTDQAIEKVSAALQKMEEQK"
     gene            1711..2004
                     /locus_tag="PKZ74_06835"
     CDS             1711..2004
                     /locus_tag="PKZ74_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM92511.1"
                     /translation="MSETTFMGVPRNKIDWKPAIDYSKCDYCMECVKFCPHRVFEVRE
                     TEEIRLIVRNPHNCVVFCRACSKICGPDALSFPDKNATTLRIKEIRKEMEGHD"
     gene            1997..2854
                     /locus_tag="PKZ74_06840"
     CDS             1997..2854
                     /locus_tag="PKZ74_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPM92512.1"
                     /translation="MIEKYAILPCNGLDKCAGVISGELAIRLCESGKNELICPVFYRV
                     SESRYNKMANVRPLLVIDGCQTRCASRLAAEKNLKVSKKLTITEEAKNHGIELKSNLR
                     LQDQEKALVEGIFKELNEEGEKVIMAPVEAITSLHFEYETYQFGKFLFRVPSNPGIYF
                     NENDGWAYIYGNHARVGVTDFVQQNLSDILYFTPPEIGDEIDQFGEAGDIESSKSVFE
                     LISPVSGKVVAINEELVKKPEMINENPYEEGWMAELELTDFESDKELLIGFEEYLELM
                     KRKVQELNE"
     gene            2847..3251
                     /locus_tag="PKZ74_06845"
     CDS             2847..3251
                     /locus_tag="PKZ74_06845"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPM92513.1"
                     /translation="MNKKVKVIPCSGIGKVYGLIARESALKTVFDLLPEKSETVCLAY
                     IVTGDKEAREKIEGFACITVDGCPKMCASKNVSIAGGNVMEEIKVLDTVKEHKGKKFG
                     SPTQLDEDGELVTDEIAAKIAKTINELNEGGK"
     gene            3252..3701
                     /locus_tag="PKZ74_06850"
     CDS             3252..3701
                     /locus_tag="PKZ74_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPM92514.1"
                     /translation="MDELKVGIISCSGEECLGGTISRLATRKVLEELKMISTVTLCLP
                     LYLAGGEEERNFANVYPTLAVDGCDKLCAKRSTEKYSGKINGSIDVSSIIGKENALNT
                     KTVRNKDLTAEHIAMAEEVASEIVKMISNLPPDSYSGKTFHGCACGS"
     gene            3775..4095
                     /locus_tag="PKZ74_06855"
     CDS             3775..4095
                     /locus_tag="PKZ74_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92515.1"
                     /translation="MSACHKEDKNPVVNPSEKLALDSLVATNKHIVTWEEIQVRAYAR
                     GENLNFEWYTNHGSMIAVDSATVLYWACPSCEGLNIIECTVSNDYGSISDTIMIQVDP
                     ENGL"
     gene            4112..5020
                     /locus_tag="PKZ74_06860"
     CDS             4112..5020
                     /locus_tag="PKZ74_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92516.1"
                     /translation="MIFALFSLNGPGQCCSAGNPLGSNGINDGLPVKEWQFLTSYKHS
                     LSKNYFHKDHQVEAPVVEKSYYDYQSISVAYGILKWLNASAEIGYFYNKTQELEPGQT
                     MDQIRAGGLGDLALNLRVLPVKTLRRTSQFIASGGIRIPVGAFEKEIDGVTVPVSLQP
                     SSGALKINTSVYYSMPLFRPEFSMNSFAFYEYSNTIEEGYFIYKYADFFQYSIGGQYV
                     YNKRLSILLNMKYEFRGKDHRENEAVVESSGSHLVLINPQVTYNIKRGWSLMVLADLP
                     LYKYVNGKQLTNSYAVQCSLYKRITF"
     gene            5107..5442
                     /locus_tag="PKZ74_06865"
     CDS             5107..5442
                     /locus_tag="PKZ74_06865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM92517.1"
                     /translation="MPQAKTELFETELQTTSMFLKALSHPARLMIVKFLADCKSCYTG
                     DITSELPLSRTTINQHLDELRKAGLIMGHITGNKTSYCINQERIGEMKAVIGAYLDEL
                     DISVPFKCE"
     gene            5484..6230
                     /locus_tag="PKZ74_06870"
     CDS             5484..6230
                     /locus_tag="PKZ74_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92518.1"
                     /translation="MKTRLFFLLLVMAIISLQASATVKSDLEAANKNGNSVFLVVTDP
                     NITGTDQALDIANKAKTSVTKSIVLQMNRSDAANTELVTKYRLAGAPLPLILVIASNG
                     VVTAGYALAQATPELLVKAVPSAKKSEVLKALSDGKSVFIVVTGKSMAEKTTIMNTCQ
                     QACVEMENNAKMIEISLDDPKEKQFLTELKVNMAAAEPTTYVINSKGQMTGTFTDDVN
                     STTLVASAKKVAASGCCPSGSGKSCGPAKK"
     gene            6240..7457
                     /locus_tag="PKZ74_06875"
     CDS             6240..7457
                     /locus_tag="PKZ74_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92519.1"
                     /translation="MKVIKLVWLELGQRKTQLMSGILAITLGISVIVAIRSISLVSEK
                     AVAINLDNLGANIMVLPQGATIDNYYSADIDAPTIPEEYVERILTSSLQGVDNLSPKL
                     TRRVDIGDYKFVLTGILPSNELASKPLWQSAGLFGAGLAASCGENPQNESLGYKDEKL
                     QRKSIDSLAMADCLLGYSAAQKLGLKEGGVLTIMGNELKVARVLTETGTIDDDRVFAH
                     LRSVQNILGIHDQVSAIEIMGCCNAISDGLLGQLRNILPDTRITTIGQIVSTQIKTNQ
                     LMNQISLIILIIILIVGGISIGNYMWANVNERRKEIGILRMIGFHKSSIYSMLLIKAV
                     FMGVVGGLAGYLAGALIAWFLGPTLAGIIVVPIPLLLLYSVLLAAGIAVVGSLLPAYF
                     ASRFEPYSNMQEI"
     gene            7454..8128
                     /locus_tag="PKZ74_06880"
     CDS             7454..8128
                     /locus_tag="PKZ74_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92520.1"
                     /translation="MTTILSTNALSKHYFHRDGLIMALDDVSISVSEGSFVTITGPSG
                     SGKTTLLMALSGLIRPTSGKIYFKEQMLETDNDTALVEFRKRYTGFIMQNFSLIPYMT
                     AIQNVMLPLSLNSISEESRKHRAVELLELAGLGNRMNHYPRELSAGQQQRVAIARALA
                     NKPAIIFADEPTGNLDPMLSVEILNFLKEINEKNRITIFMVTHSPAAGAYGNVSIKLR
                     DGRIEN"
     gene            8168..9220
                     /gene="arsB"
                     /locus_tag="PKZ74_06885"
     CDS             8168..9220
                     /gene="arsB"
                     /locus_tag="PKZ74_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPM92521.1"
                     /translation="MPSKSRLKFLDRYLTLWIFLAMFAGVFMGWAFPSVAGFWDSMSS
                     GTTNIPIAIGLIVMMYPPLAKVKYEELGDVFRNVKVLSLSLVQNWIIGPVLMFLLAII
                     FLQFNIEYMYGLILIGLARCIAMVIVWNDLAKGDRQYAAGLVAFNSIFQVLFFSIYAW
                     FFIAVLPGWFGLPVNDAVEQITIGQIAKSVFIYLGIPFIAGFLTRFILIRVKDKTWYE
                     TKFVPKISPLTLIALLFTILVMFSLKGEYIVKIPLDVVRIAIPLVIYFVIMFLVSFFM
                     SMKVGANYEQSTTLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVMIAL
                     VNVAFWFKRTYFKPVE"
     gene            9304..9723
                     /locus_tag="PKZ74_06890"
     CDS             9304..9723
                     /locus_tag="PKZ74_06890"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPM92522.1"
                     /translation="MGKKILVICTGNSCRSQMAEGFIRSFDKTLKVHSAGTHPADKVN
                     PNAVKVMKEIGIDISDHVPELVDKYVNEEWDYVITVCGGAKEVCPAFTGTVKHRLHIG
                     FDDPADAVGSEEEVMAVYRRVRDEIKVSFIEFYLKIR"
     gene            9742..>10168
                     /locus_tag="PKZ74_06895"
     CDS             9742..>10168
                     /locus_tag="PKZ74_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM92523.1"
                     /translation="MEDVKKIVREKYGEIARENAEPEKKTSCGCGCGCDDTYTIFSEN
                     YNGKDGYDPDADLGLGCGIPTDFVQIHKGDTVIDLGSGAGNDCFVARALVGEEGKVIG
                     IDMTKEMIELARKNADKLGYKNVQFRYGDIENMPVTSKKA"
BASE COUNT         2992 a         2043 c         2474 g         2659 t
ORIGIN      
        1 cctgcactgg cgctgctcct cgccggcccc tcattatcgt taccgaatat gctggttatt
       61 aggggagtga tgggaacaca aaaaaccgta atgtatgttg tgctggtcgt aattatggcc
      121 acgatcagcg gtttgattta tggcacaatc tgactttatt caaatggcta tacagatcgt
      181 catattatgt cccggaagca aatgcaaaaa gtgcaggcga atgatccgcc aggttgaaga
      241 agcagtgctg gaatcggggc ttgaagcaga agttaagatc ctggacacac tggacgacct
      301 gatgaaatac aatacatacg tgctgccggc cctggtgatc aatggcaaga tgctggcccg
      361 gggatatgta ccggaaaaaa gtatgatcat aaaggaaatg aataaactgc ccgataacgg
      421 aaagaaatat gagtcttgaa attgaaaaat acggatttgt gatcaacgac ctacggcatg
      481 tttccggaaa ggaatgcctg gaaattttaa agacagacgg gtttattttg gacgtcaggc
      541 ctgattttga acttaccagg ttgatcgacc acgagcacat cctttattac ccttatgatg
      601 agatcaggga gcacttccgg aaactgcccc acgatacctg gctcattctt gccgatgcag
      661 tcgggctcag gagtaaggaa gtagccttct ttctcaaaga gaatggattt tcaaatatcc
      721 ttcaccttgc cggtggagtg gttgaatggg aacgagacgg tcttcctgtt acactggata
      781 aaagccggag actgacagga tcttgcatgt gccagctaaa gcgcagggaa atcaaaaaat
      841 gaaagatgcc atcacgactt aacaatgagt tattatccaa ataaattcta aaaaatgaaa
      901 aagcacatta catttatgat ggcagtcatg ctaaccattt cggcaggtat gatctttggg
      961 cagtcaaata agacgagtac agaggtgctg tattttaaag ccaatttgtg taaatgcaaa
     1021 gccggggcgt gcaatgctct tcaggctgat gtcgattccg tcatcgtgaa atatttccaa
     1081 aaggaaaata ttgagttcaa agttatcaaa ctggccgatg aggcaaataa ggaattagta
     1141 tccagattca acgcaaaatc acagaccgta gtgattatta gtaaaaagaa gaagaaggaa
     1201 aatgtcattg atgcctctgc tatagttgca gattattcta aaacaaaaga taaagtaaga
     1261 tttgaagctg aaatgaggaa aaaaataagc gagagtcttg tgttttaata aaaaaaacaa
     1321 tctgtataat attagatata tgtctgaatc gaataaaccc ggttttatca tgtgcgtttg
     1381 cacaggcaaa tgtcccggat tctcccagat ggatatatgg gattttataa acagggtaag
     1441 gatcgaactg ccggtagaat acggatttat acacccgcaa ctttgtgagg aagacgggga
     1501 ccggttcctg ctggattttt taaaggctca caggaaagta gttataggcg gctgtgctcc
     1561 caatatgcag atgaagcttt tcagggatgc attcaaagaa tcgggcctgg aaataagcac
     1621 ggatctaatc cctatcgatg tgcgggatat gacaactgac caggccatag aaaaagtcag
     1681 tgcggcgctg caaaaaatgg aggagcagaa atgagcgaaa ccacttttat gggtgtcccg
     1741 cgcaataaaa tcgactggaa gcctgccatt gattattcga aatgcgatta ctgcatggaa
     1801 tgcgtgaaat tctgccctca cagggtattc gaagtcaggg agaccgaaga aattagattg
     1861 attgtaagga accctcataa ttgcgttgtt ttctgcaggg cttgctcgaa aatttgcggt
     1921 ccggatgcac tgtcatttcc ggataaaaat gcaactacac taagaattaa ggagattaga
     1981 aaggagatgg aaggccatga ttgaaaaata tgccatcctt ccctgcaacg ggctggataa
     2041 atgcgcgggt gtcatttcag gagaattagc catcaggctc tgtgagagcg gaaaaaatga
     2101 gctgatctgc cccgtatttt acagggtttc tgaatccaga tacaacaaga tggcaaatgt
     2161 gcgccctctc ttggttatcg atggatgcca aacgcgttgc gccagcaggc ttgccgctga
     2221 aaagaacctg aaagtttcaa agaaactgac catcaccgaa gaagccaaaa atcatgggat
     2281 tgaattaaag agcaatctca ggcttcagga ccaggaaaag gctctggttg aaggtatttt
     2341 taaagaattg aatgaagaag gggagaaggt gataatggca ccggtcgaag caattacctc
     2401 tttgcatttc gaatacgaga catatcaatt cggtaaattc ctattcaggg tcccaagtaa
     2461 tcccgggatt tatttcaacg aaaatgacgg ctgggcttac atatacggaa accacgcccg
     2521 ggttggcgtt accgattttg tacaacagaa tctgtccgat atcctctatt ttacacctcc
     2581 tgaaatcgga gatgaaatag accagttcgg agaagcaggt gatattgaat cgtcgaagtc
     2641 ggtctttgaa ttgatttcgc ctgtcagcgg caaagtcgtt gcaattaatg aagagcttgt
     2701 aaaaaagcct gaaatgatca atgagaatcc ttatgaagag ggttggatgg cggaacttga
     2761 attaaccgat tttgaaagcg ataaggagct tttgatcgga tttgaagaat acctggaatt
     2821 aatgaaaaga aaggtgcagg aattaaatga ataaaaaagt aaaagtgata ccctgtagcg
     2881 gtatcggtaa ggtttatggc ctcattgcaa gagaatctgc tttgaaaacg gttttcgacc
     2941 tgctccctga aaaatcagag acggtttgtc tcgcctacat tgtaaccggc gataaagaag
     3001 cgagagagaa aatcgaaggt tttgcatgca ttactgttga tgggtgccct aaaatgtgtg
     3061 cttcgaagaa tgtttcaata gccggcggga atgtcatgga agaaataaaa gtattggata
     3121 cggttaagga acataaagga aaaaaattcg gatcaccgac gcagcttgat gaagacggcg
     3181 aattggttac ggatgaaatt gctgcgaaga ttgcaaaaac gattaatgaa ctgaatgaag
     3241 gagggaaata aatggacgag ttaaaagttg gaattatttc atgcagtgga gaagaatgcc
     3301 tcggtggaac tatttcgagg cttgccacaa gaaaagttct cgaagaactg aaaatgatca
     3361 gcactgtgac actttgcctt cccctgtatc ttgccggtgg tgaagaagaa aggaatttcg
     3421 caaatgtata cccgacactg gcggtagatg gttgcgacaa gctatgcgcc aaaagaagca
     3481 cggaaaaata cagcggaaag atcaatggct ccatagatgt ttccagtatc atcggcaagg
     3541 agaatgcact gaacacaaaa accgtcagga ataaagacct caccgcagag catattgcta
     3601 tggcggagga agttgccagt gagattgtga aaatgatcag taaccttcct cctgattctt
     3661 actcgggtaa aactttccat ggctgcgcct gcggaagcta agtagaacta tatctatcat
     3721 ttacatattc ttatggaaag aaatttctgc ttgatgatat tgcttttaat actcatgtcc
     3781 gcttgtcata aagaggataa gaaccctgtt gtcaatcctt cggaaaaatt agccttagac
     3841 agcctggttg ccacgaataa gcatatcgta acctgggagg agatccaggt aagagcctat
     3901 gcgcggggag agaatctcaa ctttgaatgg tataccaatc atggaagtat gatcgctgtc
     3961 gattcagcca ccgtgcttta ctgggcctgc ccttcctgtg aagggctgaa tatcatcgaa
     4021 tgcaccgttt caaatgatta tggcagtatt tctgatacaa taatgataca agttgatccg
     4081 gagaatgggt tataaatttc tcttgttaat catgatcttt gccctctttt cattgaatgg
     4141 gcccggtcaa tgttgctctg ccggaaatcc actgggcagt aatggcatca acgatggttt
     4201 gcccgttaag gagtggcagt ttctgacgtc atacaagcat agcctttcga agaattattt
     4261 tcacaaggat caccaggtgg aagcccctgt ggtcgaaaag agttattacg attatcagag
     4321 catttctgta gcttatggga ttctgaagtg gttgaatgcc agtgccgaaa tcggttattt
     4381 ttacaataag acccaggaac tggagcctgg ccagacgatg gatcagatca gggccggggg
     4441 cctgggcgac cttgctctta atctcagggt gctgcctgta aaaactctca ggagaacatc
     4501 ccagttcatt gcatcgggtg ggatcaggat cccggtcggg gcgtttgaaa aggaaattga
     4561 cggagtgacg gtcccggttt ccttacagcc atcatcaggg gcattaaaga tcaatacaag
     4621 cgtatattat tccatgcctc tctttcgtcc tgaattttca atgaattcct tcgctttcta
     4681 cgaatacagc aatacaatag aagaaggtta tttcatttat aaatacgcgg actttttcca
     4741 gtacagcatc ggggggcaat atgtctataa taagcgactg tcgattttac tgaacatgaa
     4801 gtatgaattc cgtggcaaag atcacagaga gaatgaagca gtggtcgaat cctccggcag
     4861 tcacctggtt ctgatcaacc ctcaggtgac gtataacatt aaacggggat ggagtttaat
     4921 ggtattggct gatttgccgc tatacaaata tgtgaacggg aaacagctaa cgaatagtta
     4981 tgctgtgcaa tgttcattgt ataagaggat cacattctaa gccaaaattc aatctaaacc
     5041 ataaaaaaat taacgtattt atacgtcaat tcaaaaatga tgtattttta cgtcaaaatt
     5101 aattttatgc cacaagctaa aaccgagtta ttcgaaaccg aattacaaac cacctctatg
     5161 ttcttaaaag cgttaagcca tccggccaga ctgatgattg tcaaattcct ggccgattgc
     5221 aaaagctgtt ataccggaga tatcacttct gagcttccgc tgagcaggac taccatcaac
     5281 cagcatctgg atgaacttag aaaagccggg ttgatcatgg gacatattac aggaaataag
     5341 accagttatt gtatcaacca ggaacgaatc ggtgaaatga aagccgttat tggcgcttac
     5401 ctggatgaac tggatatttc tgttcctttc aaatgtgaat gatatttaaa attataaaaa
     5461 ccaaattttt taaagaattc attatgaaaa cacgtttatt tttcctttta ctggtcatgg
     5521 caatcatcag cctgcaagct tctgcaaccg taaaaagcga cctggaagca gcaaacaaaa
     5581 atggtaattc cgttttcctt gtggttaccg atccgaatat taccggaacg gatcaggcac
     5641 tggacattgc aaataaagcc aagacaagcg ttaccaaatc cattgttctg caaatgaacc
     5701 gttctgatgc agccaacacg gagttagtca ccaaataccg tcttgccgga gcgccattgc
     5761 cgcttatcct tgtaattgct tccaatggtg tggtaaccgc gggatatgcg ctggcccagg
     5821 ccacacctga attactggta aaagctgtcc catcagcaaa aaagtctgaa gtgctgaagg
     5881 ccctgagtga cggtaaatct gttttcattg tcgttacagg caaatcaatg gcagagaaga
     5941 ccacgatcat gaatacatgt cagcaagcgt gcgttgaaat ggagaataac gccaaaatga
     6001 ttgaaataag ccttgacgat ccaaaggaga aacaatttct gacggaacta aaggtaaata
     6061 tggcggctgc agaacccaca acttatgtca ttaactccaa gggccagatg accggtactt
     6121 tcactgacga tgtaaactcc accacactgg ttgcttcagc taagaaagta gccgcatcgg
     6181 gttgctgccc ttcgggtagc ggaaaaagct gcgggcctgc aaagaaataa tctgatccta
     6241 tgaaagttat taaattggtt tggttggaac tcggacagag aaaaacacaa ctaatgtcgg
     6301 ggatcctggc aataacatta gggatctcag tcatcgtagc catcaggtca atttcgctgg
     6361 tttccgaaaa agcagtggcg atcaacctgg ataaccttgg cgccaacata atggtgcttc
     6421 cccagggggc caccatcgat aattattatt cagccgatat tgatgcgcca acgattcctg
     6481 aagagtatgt tgaaagaatc ctcacttcct cattgcaggg agtagataac ctcagcccaa
     6541 aacttacccg gcgggtcgat attggtgatt ataaatttgt attgaccggc atacttcctt
     6601 cgaatgaact ggcatctaaa ccactatggc aatcggccgg actctttgga gccgggctcg
     6661 cagcaagttg cggtgaaaat ccacaaaatg aatcccttgg ctataaagat gaaaaactgc
     6721 aacgcaagtc tattgattcg ctggcaatgg ccgactgcct gcttggttat tcagcagctc
     6781 aaaaacttgg cctgaaagag gggggcgttt taacaattat gggtaatgaa ctgaaggttg
     6841 ccagggtatt gacagaaacc gggactattg atgatgacag agtttttgct cacttaagat
     6901 ctgtgcagaa tatcctgggt attcatgacc aggtcagtgc cattgagatc atgggttgct
     6961 gcaatgcgat atctgatggc ttattgggtc agctcagaaa cattttgcct gacacgcgga
     7021 tcaccaccat cggtcagatc gtatccacac aaattaaaac gaaccagttg atgaaccaga
     7081 tttcgctcat cattctgatc attatcctga tcgtgggcgg gatctccatt ggaaactata
     7141 tgtgggccaa tgtcaatgag cgtagaaaag agatcggcat cctgcgaatg atcggctttc
     7201 acaagtcttc catttattca atgctgctga ttaaagcggt ttttatggga gtggtaggag
     7261 gtttggccgg ttaccttgcc ggcgcattga ttgcctggtt cctgggtccc acactggctg
     7321 gcatcattgt cgtgcccatt ccgttactct tactctactc ggtactgctt gcagcgggta
     7381 tcgctgtcgt cggctccttg cttccggctt actttgcctc acggtttgaa ccctattcaa
     7441 atatgcagga aatatgacga cgatcctcag tactaatgcc ttatcgaagc attattttca
     7501 cagagacgga ttgattatgg cgcttgacga tgtatccatt tctgtttctg aaggctcctt
     7561 cgtcaccatt accggaccct ccggatcagg aaagaccacg ctgttaatgg cattaagcgg
     7621 attaatccgt ccgacctcgg ggaagattta ttttaaggaa cagatgctgg agacggataa
     7681 cgacacagcg ctggtcgaat tccgtaaaag atacaccggt tttatcatgc agaatttttc
     7741 gctgatccca tatatgaccg cgatacaaaa tgtaatgctt cctctgtcac tcaatagtat
     7801 atcggaagaa agccggaaac acagagcggt agaactcctc gaactggctg gacttggaaa
     7861 ccggatgaac cattatccac gggagttatc agccgggcaa cagcaaagag tggccattgc
     7921 gagggcgctg gctaataagc cggcgattat atttgctgat gaaccgacag ggaatctgga
     7981 tcccatgcta tctgtggaga ttttgaattt tcttaaagaa attaatgaga aaaacaggat
     8041 cactattttt atggttacgc acagtccggc cgccggtgcc tacggtaatg tttcaattaa
     8101 gctgagggat ggcaggattg aaaattagca agtttaataa ataatgaatt tcatcaagaa
     8161 ttgaaatatg ccatcaaaat cacgattaaa attccttgac cgttacctga ccctctggat
     8221 tttcctcgcc atgtttgcgg gggttttcat gggttgggca ttcccctccg ttgccggatt
     8281 ctgggacagc atgtcgagcg ggacaaccaa tatccctatt gccattggac tgatcgtcat
     8341 gatgtacccg ccactggcga aagtaaagta cgaagaactc ggagatgttt tcagaaacgt
     8401 taaggtactg agcctgtcgc ttgttcagaa ctggatcatc gggccggtcc tgatgtttct
     8461 tctggcgata atttttctgc aattcaacat cgaatacatg tacggtctca tcctgatcgg
     8521 gctggcccgg tgtatcgcta tggtgattgt atggaacgac ctggccaaag gcgaccggca
     8581 gtacgctgcc ggactggtgg catttaattc catcttccag gtattgttct tttcgattta
     8641 tgcctggttc tttattgcgg tccttcccgg ctggttcggc ttaccggtaa atgacgctgt
     8701 tgagcagatc accatcggcc agattgccaa aagcgtgttc atctacctgg ggatcccgtt
     8761 tatcgccggc tttttaaccc gatttatcct gatcagggtg aaggataaga cctggtacga
     8821 aacgaaattt gtcccgaaaa tcagtccctt aacactgatt gcattgttgt tcaccatcct
     8881 ggtgatgttc tccctgaaag gcgaatacat cgttaagatc ccgctggatg tggttcggat
     8941 cgcgatcccg ctggtcatct attttgtgat catgttcctg gtttccttct tcatgagcat
     9001 gaaagtcggg gctaattatg agcagtcgac cacgttgtcg tttaccgcag caagcaacaa
     9061 ttttgaactt gctatcgcgg tggctattgc tgtatttggg attaattccg gagaggcttt
     9121 cgctgctgtg atcggcccgc ttgttgaagt gccggtaatg attgcgctgg taaatgtggc
     9181 gttctggttt aaaaggacct attttaaacc ggttgagtga aatcatctta aacgtatatt
     9241 caaaccaaaa ccgcaaggag acgttaacat tgagtacttt tgcgtagaag ttagcatagt
     9301 tttatgggta agaaaatttt agtaatttgt acgggaaaca gttgccgcag ccagatggcg
     9361 gaaggtttta taaggtcgtt cgataaaacg ctgaaggttc attcagcagg aacgcatccg
     9421 gccgataaag tcaatcccaa cgcggtgaaa gtgatgaaag agatcggcat cgatatcagc
     9481 gatcatgttc cggaactggt tgataaatat gttaatgagg aatgggatta tgtgatcacc
     9541 gtttgcggcg gtgcaaaaga agtctgcccg gcattcaccg gcacagtgaa acatcgactg
     9601 catatcgggt ttgatgatcc ggcagatgcg gtaggcagcg aagaggaggt gatggctgtg
     9661 taccggaggg tgcgggatga gataaaagtt agctttattg agttttattt gaaaattaga
     9721 taatttggaa attgggtaat tatggaagat gtaaaaaaga ttgtccgtga gaaatacggc
     9781 gagatagcca gggaaaatgc cgaacctgaa aagaaaacct cttgcggttg cggttgcgga
     9841 tgtgatgata cgtataccat tttcagcgaa aattataacg gaaaggatgg ctatgatccg
     9901 gatgccgacc tgggcctggg ttgtggcatt ccgaccgatt ttgttcagat tcataaaggc
     9961 gatacggtaa tcgacctggg ctccggcgcc ggcaacgact gttttgtcgc ccgtgcactg
    10021 gttggagaag agggcaaggt catcggcatt gatatgacga aagaaatgat agaactggca
    10081 aggaaaaatg cagataaact gggctataaa aatgtccagt tccgctacgg cgatatagag
    10141 aatatgccgg tgacatccaa aaaagccg
//
LOCUS       DAOPNH010000103        10136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7181_length_10136_cov_7.323281, whole genome shotgun sequence.
ACCESSION   DAOPNH010000103 DAOPNH010000000
VERSION     DAOPNH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_10136_cov_7.323281"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1013)
                     /locus_tag="PKZ74_06900"
     CDS             complement(21..1013)
                     /locus_tag="PKZ74_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPM92524.1"
                     /translation="MQLSDQNSENRWLVVVNPNAGKKRGEKDWPEISGLLAAAGIDFH
                     HEFTSHRNHAVELTCEFISKGFRKIIVVGGDGTLNEVVNGIFRQDEVEPNDITLGMIM
                     VGTGNDWGKMYNLKENYKDAIHIIREGNIFLQDAGVVIYLENDIEQVRYFVNSAGMGY
                     DALVVQMTNQGKEKGRGGTMAYMSSLLRGLFRYNLAYLDIEVDGKRVYKGKVFSMSVG
                     ICKYSGGGMIQLPFAVADDGLLDVTLFKKVSKLTVIRHLKKLYSGRFTHLPFVDTYQG
                     KTISVISAPHNPSFLETDGESLGNGPFTFHLIPRCLRVITAETRHPKSEKFLAE"
     gene            complement(1013..2674)
                     /locus_tag="PKZ74_06905"
     CDS             complement(1013..2674)
                     /locus_tag="PKZ74_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPM92525.1"
                     /translation="MKSDKIIFWLYYFSLGLMLVSLPFSNFFMSVSQFCLVGTFILHG
                     MVKEDVVRFFSENNRVKQVLLFLPYGIFWILKSVAGKFKEFFRRENSPAWIFSSIYLL
                     HLVGLFFTTDFDYALKDLRIKLPLFIIPLMLSTTDLLNRKGFRFLMYLFSAAVFTGTL
                     ISTYHYLTMDFTDSRELSRYISHIRFSLLIDLAIFFLAYIAFRKSDIPRWPRYIMAAI
                     ALWMVAFLALAAFMTGLVVFFLTAAVVLLYFILTRQGIVLRLVTLSGIILIFTVAFLF
                     VMNIGRDVNRVYPVDEGSLEKTTALGNPYWHDLSNPQTENGYYVWHYVATDELREAWN
                     RRSQYSFDGKDKAGQEIRYTLIRFMTSKGLRKDAEGLGKMSDAEVAMVEDGTASIVYV
                     SKSNLYVRIYKIFWEYGRYQETHNASGHSVMQRLEFWKASVNIIRDNWITGVGTGDLE
                     KAFQEEYVKSGSLLDPEYRWRSHNQFLAIFASFGVLGFAWFLFSLLFPALRLGKFHDY
                     HYLSFFIIIMLSMLTEDTLETQAGVTVFAFFSSFYLFSKKFIDIL"
     gene            complement(2743..4206)
                     /locus_tag="PKZ74_06910"
     CDS             complement(2743..4206)
                     /locus_tag="PKZ74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPM92526.1"
                     /translation="MFGKSEINFRQILRLIGVILVIEGVFMLTGLPFSLYYQDDKPFT
                     LLYSFIITSASGGILLFFTRSDKDNTINKREGFLVVSLAWVVMSLFGTVPFLLSGAIP
                     SFTDAFFETMSGFTTTGASILSDVEVLPKGILFWRSMTHWIGGMGIIVLTIIILPFLG
                     IGGMQLYVAEMPGVSKDKLHPKISETAKRLWGIYIVLTAIQTILLVLGKMPLLDSLCH
                     AFGSMATGGFSTRNASIGAYSPYIQYVVIVFMFLAGMNFTLHYFIISGRFKEVWKNEE
                     FRKYLYISLGFSIIIAAFLVIKTDAGYAKAFRDALFQVVSIVTTTGYATTDYLVWPGA
                     LWFFIFLMMFVGGMAGSTGGGVKVVRQLLLFKNAGKELKRAIHPQGVIPVRLNHEAVS
                     QDVIYKVMAFFQLYIIVFIFGAMILSFLGLDFESAIGASISALGNIGPGLGLVGPVGN
                     YGFVPDAGKWLLSFMMLLGRLELFTVLLLITPYFWKR"
     gene            complement(4250..5590)
                     /gene="trkA"
                     /locus_tag="PKZ74_06915"
     CDS             complement(4250..5590)
                     /gene="trkA"
                     /locus_tag="PKZ74_06915"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPM92527.1"
                     /translation="MRIIIAGDGEVGLYLAQELSKEDHSITMIVPSEDLVKTIESHSD
                     VMATVGDPTLISVLKRSNVKKADLLISGLHEERINILTAALGKKIGAIKTIARINTME
                     YLSDENRRMFEELGVDHLVCPEDIAANEVVDLLRQTAATETFDFSQGKLSLFLLRLEK
                     NAPVLNKTLDQIAKEFVHLHFRAVAIHRDRKTIIPKGDDKFLENDLAYVITKPQGIND
                     LLKLSGKSKISIHNLMLLGGGRVGRTVARRLEHSMNIKLIEKDKDRAIQLANTLDHCL
                     VIHGDARDIQLLEEEDISGMDAFVALTDNSETNILACLHAKKFGVPRTIALVENIDYI
                     DISQNIGIDTIVCKKLSTASNIIRFTLISEVTSIKCLSGINAQVLEVVTKPHSPVTKA
                     PIRKLKFPPGAIIGGIIRGSDSFIAVGDFQIQEGDKVVVFALPDALHAVDNLFH"
     gene            5703..6896
                     /locus_tag="PKZ74_06920"
     CDS             5703..6896
                     /locus_tag="PKZ74_06920"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPM92528.1"
                     /translation="MKTIDNFNFNGKKALIRVDFNVPLDENYHITDTTRIDAAIPTLK
                     KILGDGGAVILMSHLGRPKEGPAEKYSLKHLLPYLNKTLNLTVKFAGDCIGEEAKEMA
                     GKLKGGEVLLLENLRFYKNEEKGDEEFAAKLAQLGDVYVNDAFGTAHRAHASTAIIAK
                     FFPENKLFGYIMEEEVKAIDKVLRNAERPFTAILGGAKVSGKIEIINNLIDKVDNLLI
                     GGGMMFTFIKALGGEVGSSLVEEDLLQLARETSDKAEANNVKIYIPQDAVIASAFQND
                     AEKMECGSTAIPEGWMGLDIGPKAIAAYREVILNSKTILWNGPMGVFEMPNFEPGTKA
                     IAEAVAEATQNGAFSLIGGGDSVAAVNKYDLKDQVSYVSTGGGAMLEYIEGKELPGVA
                     AIKGR"
     gene            complement(6897..7394)
                     /locus_tag="PKZ74_06925"
     CDS             complement(6897..7394)
                     /locus_tag="PKZ74_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPM92529.1"
                     /translation="MTLLDFIILLPIVWLCIRGFMKGLIIELATLAGMALGILAAYYF
                     AADLQALMQDYFSLSEKGTRIVAYILIFLVVLFTVWLIGKLVEKSVDLMAMGWLNKLL
                     GAIVGIIKGVVIVCVALYLVEKYDTRQKLIKPEVKEKSMFYQPAMDAVRFVVGNRLSP
                     ARSDL"
     gene            complement(7391..8137)
                     /locus_tag="PKZ74_06930"
     CDS             complement(7391..8137)
                     /locus_tag="PKZ74_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92530.1"
                     /translation="MIEARNISLTFGENKVLDDVSFTFEQGKTNLVIGQSGSGKTVLV
                     KCIVGLLQPDEGEIHYNGRDFLSMGINEKRKLRQEIGMLFQGGALFDSMTVEQNVMFP
                     LNMFTKMSLAEKLDRVNFCLERVHLEGRNKLMPSELSGGMIKRVAIARAISLNPSYLF
                     CDEPNSGLDPITSGVIDELIKEITEEFNTTTVINTHDMNSVLMIGEKIAFIYQGKIGW
                     SGTRHEILHTGNPELNDFIFATELTRRIRR"
     gene            complement(8137..8868)
                     /locus_tag="PKZ74_06935"
     CDS             complement(8137..8868)
                     /locus_tag="PKZ74_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92531.1"
                     /translation="MVSLLGKYILLMAQVFRKPAKWRVFWDQLMSEFQKIGVESMGIV
                     AIISLFMGAVLTMQVAFNIDSPLVSPIIIGFTVRQSVILEFAPTVISLILAGKVGSRI
                     ASEIGTMRVTEQIDALQTMGVNSANYLIFPKMIAAILFNPVLIVMSMFLGLIGGLVAC
                     LLLDLTSFTVYVDGLQAWYEPWSVSYALIKTVIFAFIIVSVSGFNGYIIKGGALEVGN
                     ASTKAVVQSSILIILFNLLLTEILL"
     gene            complement(8890..9060)
                     /locus_tag="PKZ74_06940"
     CDS             complement(8890..9060)
                     /locus_tag="PKZ74_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92532.1"
                     /translation="MNFNLELGTRNLEPGTWNPELGIRNSEPGTWNPELGTRNLEPGT
                     WNPELGTRNFAL"
     gene            9169..9375
                     /locus_tag="PKZ74_06945"
     CDS             9169..9375
                     /locus_tag="PKZ74_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92533.1"
                     /translation="MKRLRITTLFLIVIFLALVAGSCASNRSPVRHYKPKRPGGCDCS
                     RWSYHQPSNPNLFYAGEENASGRA"
     gene            9347..9967
                     /locus_tag="PKZ74_06950"
     CDS             9347..9967
                     /locus_tag="PKZ74_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92534.1"
                     /translation="MKKTLREGLSEYLPEKALDVVMEWIGTRPIHVKLTRNRSSKLGD
                     FRPPQHDRINRISLNAGMNPYEFLITFTHEIAHMLTWEKYGRKVLPHGSAWKGQYAAL
                     LEELIVKDIFPPDIRKIVVHQVRNPKSTSKCDTMLTRALQPHDEEPQGIFLEDLPMGA
                     SFRMQNGRRFIKQDRLRKWYRCLSLDNRRLYRISPVARVIPEEDKH"
BASE COUNT         2685 a         2566 c         2480 g         2405 t
ORIGIN      
        1 gatttttaac agatttatta ttattccgcc aggaattttt cactcttcgg atgccgggtt
       61 tcagcggtga tcacccggag gcaccgcggg ataagatgga aggtaaaagg gccatttccc
      121 agcgattcgc cgtcagtttc aagaaaagat ggattatggg gggctgagat aaccgaaatg
      181 gttttcccct ggtaagtatc aacgaagggc agatgggtaa aacggccgga atagagtttc
      241 ttaaggtgcc ttataaccgt tagcttcgat acttttttaa agagggtaac atccagcaaa
      301 ccgtcgtcag cgacggcaaa aggcaactgg atcatcccgc ctccgctgta cttgcagatc
      361 ccgacgctca tgctgaagac ttttcctttg tagacccttt ttccatccac ttcaatatcg
      421 agataagcaa gattataacg gaacaatccc ctgagaaggc tcgacatata agccatagtg
      481 ccaccccgtc ccttttcctt tccctggttg gtcatttgca caaccagtgc atcgtatccc
      541 atccctgcgc tattcacgaa ataacgaacc tgttcaatat cattctcaag gtaaatgacc
      601 actcccgcat cctgcaaaaa aatatttccc tcccggataa tatgaatagc atccttataa
      661 ttctccttca ggttatacat ttttccccag tcattgcccg taccgaccat gatcattccc
      721 agggtgatat cgttgggttc aacctcatcc tgcctgaaga tcccgtttac cacttcattt
      781 aaagtgccgt ctccacccac cacaattatt ttccggaatc ctttgctgat aaattcgcag
      841 gtcagttcga ccgcatggtt acgatgggac gtgaattcgt gatggaagtc aatgccggct
      901 gcggcaagaa gaccggatat ctcgggccag tctttttcac ctcttttttt tccggcattc
      961 gggttgacga ccacgagcca ccgattttcg ctgttttgat cactaagctg catcagagga
     1021 tgtcgataaa ttttttactg aacaggtaaa aggagctgaa aaaagcaaaa acagtaacgc
     1081 cggcctgggt ttccagtgta tcttcggtga gcatcgacag cattatgatg ataaagaaag
     1141 aaagataatg gtaatcgtgg aacttgccga ggcgtaatgc aggaaacagc aggctgaaca
     1201 ggaaccacgc gaagcccaaa acaccgaaag aggcgaaaat tgcaaggaac tggttatgcg
     1261 accgccatct gtactcggga tcgagcaagg agcccgattt gacatattct tcctggaatg
     1321 ctttttccag gtcgcctgtt ccaaccccgg tgatccaatt gtcacggata atattgaccg
     1381 atgctttcca gaactccagg cgttgcatca ccgaatgccc gctggcatta tgggtctcct
     1441 gataccgtcc gtattcccag aagatcttgt aaatcctgac atataaattg gatttgctta
     1501 cataaacgat gctggctgtt ccatcctcca ccatagctac ttcagcatcg ctcattttac
     1561 caagcccttc ggcgtctttc cgcaaacctt tggaggtcat gaaacggatc agggtgtatc
     1621 tgatttcctg gccggctttg tcttttccat cgaaagaata ttggctgcgc cggttccacg
     1681 cctccctcag ctcatcggta gccacataat gccagacata ataaccattt tcggtttgag
     1741 gattggatag atcgtgccag tacggattgc caagggcggt ggttttttca aggcttccct
     1801 catccaccgg gtaaacccgg ttcacatcgc ggcctatatt catcacaaat aaaaaagcta
     1861 cagtaaaaat caggataatt ccgcttaaag tgaccagccg gagcacgatt ccctgccggg
     1921 tcaaaataaa ataaaggaga actaccgctg cggtaaggaa aaaaacgacc aggccggtca
     1981 taaacgcagc cagtgcaagg aaggcgacca tccagagggc gatggcagcc attatgtagc
     2041 ggggccacct cgggatatcg cttttccgga aagcgatata ggccaggaaa aatatagcca
     2101 gatcgatcag caggctgaac cggatatggg aaatatatcg cgacaactcc cggctgtcgg
     2161 tgaaatccat agtcaggtaa tggtaggtgc tgatcagcgt accggtaaaa acagcggccg
     2221 aaaacaagta catcaggaaa cggaatccct tcctgttgag cagatccgtc gttgataaca
     2281 tcagcgggat gataaacagc ggcagtttta tccggaggtc tttcagggca taatcgaaat
     2341 cggtggtgaa gaataatccg accaggtgga gcaggtagat ggaagagaag atccaggcgg
     2401 gagaattctc acggcggaaa aactctttga actttcctgc gactgacttc agaatccaga
     2461 aaatcccgta aggaagaaaa agaagaactt gtttaaccct gttgttttct gagaagaacc
     2521 ttaccacatc ttctttcacc attccatgaa ggataaaggt tccgaccagg caaaactggg
     2581 aaacgctcat aaaaaaattg gaaaacggca gggagaccag catcagaccc agggagaaat
     2641 aataaagcca gaagatgatc ttatccgatt tcataagcgc ttttcagggg ggtatttaca
     2701 acgaaggtaa caagtatttc catggaatcg gtttcttatc gcttatcttt tccagaagta
     2761 aggggtgatc agtaacaaaa cggtgaacag ttcaagcctg ccaagcagca tcataaacga
     2821 taacagccat tttccggcat caggaacaaa accataattt cctaccggtc cgaccaaccc
     2881 cagtccggga ccaatattac ccagggcgga aatactggcg ccgattgccg actcaaaatc
     2941 aagaccgagg aaagacaata tcatggcgcc aaagataaag acgataatgt acaactggaa
     3001 gaatgccatc actttgtaga tgacgtcctg tgaaaccgcc tcatggttta gcctgaccgg
     3061 aataaccccc tggggatgga tcgctctttt caattccttt cccgcattct taaataaaag
     3121 caactgcctt accactttca cgccaccgcc tgtggagccg gccattccgc ctacaaacat
     3181 catcagaaat ataaaaaacc agagcgcgcc gggccatacc aggtaatcgg tcgtggcata
     3241 tcctgtagtc gtgacaatgg aaaccacctg gaacagggca tcccggaatg ctttcgcata
     3301 ccctgcatcg gtctttatta ccaggaaagc ggcaattata atactgaacc ccagtgagat
     3361 ataaagatac ttccggaatt cctcgttctt ccatacctct ttaaaccttc cggagataat
     3421 gaagtaatga agggtaaaat tcatcccggc aaggaacata aaaacaatca caacatattg
     3481 tatgtaaggc gaataggctc cgatgctggc gttcctggtt gaaaatccac cggtagccat
     3541 cgacccgaaa gcatggcaaa ggctgtcgag caggggcatc tttcccaaaa ccagcaaaat
     3601 cgtctgtata gcggttaaaa cgatatagat cccccacaac cgtttggccg tttcggaaat
     3661 tttcgggtgc agcttatctt tggaaactcc cggcatttcg gctacataaa gctgcatccc
     3721 gccaattccc aggaatggca ggatgataat tgtcagtaca atgatcccca tgccgccgat
     3781 ccagtgggtc atactccgcc agaagaggat cccttttggc agcacttcaa cgtcgctcag
     3841 aatggaggct ccggttgtcg taaatcccga catggtttcg aagaaagcat cggtgaaaga
     3901 agggatagcg ccgctcagca aaaacgggac agtcccgaac aggctcatta ccacccaggc
     3961 caggctgacc accaggaatc cttctctctt gtttatcgtg ttgtctttat cagaacgggt
     4021 gaaaaacaat aaaatccctc ccgatgcgct ggtgatgatg aacgagtata acagggtgaa
     4081 aggtttatca tcctggtaat aaagagaaaa gggcaatccg gtcagcatga aaacaccttc
     4141 gataacgagg atcacgccaa taaggcgaag aatctgcctg aaatttattt ccgatttgcc
     4201 gaacatacca agggataggt caagttattt tttccgaaag tctgaatctc taatggaaaa
     4261 ggttatcgac agcgtggagg gcatcgggca gggcaaagac caccaccttg tcgccttcct
     4321 ggatctgaaa atcgccgacg gcaatgaagc tgtcggatcc ccggataatc ccgccgatga
     4381 tggcccccgg gggaaatttc agcttccgga tcggagcttt ggttaccgga gaatggggtt
     4441 ttgtcaccac ctcaaggacc tgggcattga tcccgctgag gcatttgata ctggtcacct
     4501 ccgaaatcaa agtgaagcgg atgatattac tggctgtact gagtttttta cacacgatag
     4561 tgtcgatgcc gatattttgt gagatgtcga tatagtcgat attctcgacc agcgctatgg
     4621 tcctgggaac accgaacttt ttggcatgga ggcaggccag gatattggtt tccgagttgt
     4681 cggtgagggc aacaaatgcg tccatccctg aaatatcctc ttcttcgagc agttgaatgt
     4741 cgcgcgcatc gccgtggatc accaggcagt ggtcgagtgt attggccagc tggatagcgc
     4801 ggtctttatc tttttcgatg agtttaatgt tcatcgaatg ttccagcctc cgggctacgg
     4861 tacgcccgac ccttcctccg ccgagcagca tcaggttatg gatagagatt ttcgatttcc
     4921 cgctcagttt cagcaggtcg ttaatcccct ggggttttgt gatcacgtat gccaggtcat
     4981 tttcaaggaa tttatcgtcc cctttcggga tgatcgtttt ccggtcgcgg tggatggcta
     5041 cggccctgaa gtggagatgg acaaattctt tggcgatctg gtcgagcgtc ttgttcagaa
     5101 ccggggcgtt cttttcaagg cgaagcagga aaagggaaag ctttccctgt gaaaaatcaa
     5161 acgtttcggt ggccgccgtc tgccgaagca ggtccactac ctcatttgcg gcaatatctt
     5221 caggacagac caggtgatcg acgccgagct cctcgaacat cctcctgttc tcatcggaaa
     5281 ggtattccat tgtgttgatg cgggcgatgg tcttaatagc gccgatcttt ttccccagtg
     5341 cggcggtcag gatgttgatc cgctcttcat gcagcccgga gatcagcaga tcggcttttt
     5401 tgacattcga ccgtttcagc accgagatca gcgtcggatc accgaccgtt gccataacgt
     5461 cgctgtgtga ttcgatggtt ttgaccagat cctcgctggg gacgatcata gtgatgctat
     5521 gatcttcttt ggataattcc tgggccaggt agagtccgac ttctccatcc ccggcaatga
     5581 tgattctcat cgtaatgacg aattaaataa ggggcaaagg tagagtattt tcccggtata
     5641 ttcctatttt tgcagtgccg aagatggaga ctccatctcg agtgaattaa caccaattcg
     5701 acatgaaaac aatagacaat ttcaatttta acggaaagaa ggccctgata cgggtcgatt
     5761 ttaatgttcc cctggatgaa aattatcata ttaccgacac tacccgcatc gatgcagcca
     5821 tccccacgct gaaaaagatc ctcggtgacg gcggcgcggt gatcctgatg tcgcacctgg
     5881 gccgcccgaa agaaggccct gccgaaaaat attcgctgaa acacctcctg ccctatctga
     5941 acaaaacgct taacctgaca gtaaaatttg ccggtgattg tatcggcgaa gaagcgaaag
     6001 agatggccgg caagctgaaa ggcggggaag tgctgttact ggaaaacctg cgtttttata
     6061 aaaatgaaga aaaaggcgat gaagagtttg ccgccaaact ggcacaactg ggcgatgttt
     6121 acgtgaatga cgcctttggg acggcgcacc gcgcacatgc ctctacggcg atcatcgcta
     6181 agttcttccc ggaaaacaaa ctgttcggtt acataatgga agaagaggtg aaagccatcg
     6241 acaaggtgct gcggaacgcc gagaggccgt tcaccgcgat cctgggcggg gcgaaggttt
     6301 cgggaaagat agagatcatc aacaatctca tcgataaggt cgataacctg ctgatcggcg
     6361 gcgggatgat gttcaccttc atcaaagccc ttggcggaga ggtgggcagc tcgctggtag
     6421 aggaagacct gctgcagctg gcccgcgaaa ccagcgacaa agccgaagcc aacaatgtta
     6481 aaatctatat cccgcaggac gctgtcattg ccagcgcctt ccagaacgat gcggaaaaga
     6541 tggaatgcgg atcgacggcc ataccggaag gatggatggg ccttgatatc ggcccgaaag
     6601 ccatagctgc ttaccgcgaa gtgatcctga actctaaaac gatattgtgg aatggcccga
     6661 tgggtgtttt cgagatgccg aatttcgaac cggggaccaa agccattgcc gaagctgttg
     6721 ccgaagccac gcagaacggc gctttttcac tgatcggcgg tggtgactcc gtggcagccg
     6781 tcaacaagta cgatctcaaa gaccaggtca gctacgtcag caccggcggc ggcgccatgc
     6841 tcgaatacat cgaaggcaaa gaactcccgg gagtggcggc gattaaaggt cgttagttaa
     6901 aggtcgctcc tggcggggct caagcggttt ccgaccacaa agcgcaccgc atccatcgcc
     6961 ggctgataaa acatcgattt ttctttcact tccggtttga ttagcttctg gcgggtatcg
     7021 tacttctcca ccaggtatag cgcaacacaa acgatcacaa ccccttttat gatgccgacg
     7081 atagctccga gcagtttgtt cagccatccc atcgccatca ggtcgaccga tttttcgacc
     7141 agcttgccga tcagccatac cgtgaacagc acgaccagga agatcaggat gtaggcgacg
     7201 atccgggtcc ctttttctga aagggagaaa taatcctgca tgagcgcctg caggtcggcg
     7261 gcaaagtagt aggcggccag tatccccagc gccatgccgg caagggtggc cagctcgatg
     7321 atgagcccct tcataaaacc gcggatacaa agccagacga taggtagaag aatgataaaa
     7381 tcgaggagtg tcatcgcctg atcctgcggg ttagttcggt ggcgaaaatg aagtcgttca
     7441 gttccgggtt gccggtatga aggatctcgt gccgggtccc gctccacccg atcttcccct
     7501 ggtaaatgaa agcgatcttc tcgccgatca tcagcaccga attcatatcg tgtgtattga
     7561 tcaccgtggt ggtgttgaac tcctccgtta tctctttgat cagttcatcg atgaccccgg
     7621 aggtgatcgg atcgaggccc gaattgggct cgtcgcagaa caagtaactg ggattcaggg
     7681 agatggccct ggctatggcc acccttttga tcatcccgcc actgagttcc gatggcatca
     7741 gtttgtttcg accctcaagg tgtacccgtt caaggcagaa attcacccgg tcgagttttt
     7801 ccgccaggct cattttcgta aacatgttta gcgggaacat cacattctgt tcgacggtca
     7861 tgctatcgaa caacgcaccg ccctggaaaa gcatcccgat ctcctgccgg agttttcgct
     7921 tttcattgat ccccatgctc agaaaatccc tgccgttgta atggatctcc ccttcgtcgg
     7981 gttgcaacag gccgacaatg catttcacca gcaccgtttt acccgagccg ctctgcccga
     8041 tgaccaggtt ggtcttcccc tgttcaaaag tgaatgaaac gtcgtcgagg actttgttct
     8101 cgccaaaggt gagcgatatg ttccgggcct cgatcatcag agcaggattt cggttaaaag
     8161 gaggttaaat agaatgatga ggatactgct ctgtaccacc gctttggtac tggcattccc
     8221 gacctcgagg gctccgcctt tgatgatgta tccgttaaag cctgatacgc tgacaatgat
     8281 aaaagcgaag atgacggttt tgatgagcgc atagctgacc gaccagggtt cgtaccaggc
     8341 ctgcaggcca tcgacatata cggtgaaaga tgtcagatcc agcaacaggc aggctaccag
     8401 ccccccgatc aggccgagga acatgctcat gacgatcagt accgggttga agaggatggc
     8461 ggcaatcatc ttcggaaaaa tcaggtaatt tgcactgttc acccccatgg tctgcagtgc
     8521 gtcgatctgt tcggtcaccc gcatcgtacc gatctcggaa gcaatccgcg atcccacttt
     8581 gccagccagg atcaggctga tgacggtcgg tgcaaactcg agaatgaccg actgcctgac
     8641 ggtaaacccg atgatgatcg gcgataccag ggggctgtcg atattgaatg ccacctgcat
     8701 cgtgaggacc gcccccatga acagggaaat gatcgccacg atccccatcg actccacgcc
     8761 gatcttctgg aactcgctca tcaactggtc ccagaaaaca cgccacttgg ccggttttct
     8821 gaagacctgc gccatgagca gaatatattt tccgagtaga ctcaccataa tgagttcaaa
     8881 gtgcaaagtt caaagtgcaa agttccgggt tccgagttcc gggttccaag ttccgggttc
     8941 caggttccgg gttccaagtt ccgggttcca ggttccaggt tccgagttcc ggattccaag
     9001 ttccgggttc caggttccgg gttccaggtt ccgagttccg agttccaagt taaagttcat
     9061 agttgagcaa aggatttcgt tatccgtcat ttcaaaatta caaaatattg catatggtca
     9121 ggataacaat ttttgtaacc tttgcccgtt attttgattt ggtaccgaat gaaacgttta
     9181 cggattacaa cattattcct gatagtcatc ttcctggccc tggtcgcagg ttcatgtgca
     9241 tcaaaccgtt cgccggtgcg ccattacaaa ccaaaaaggc ccggaggatg cgattgttcc
     9301 cgctggtcgt accatcaacc ttcaaatccg aatttatttt atgccggtga agaaaacgct
     9361 tcgggaaggg cttagcgaat atttacccga aaaagcgctg gatgttgtga tggagtggat
     9421 agggaccagg ccgatccatg taaaattaac ccgaaaccgt agcagcaagc tgggcgattt
     9481 ccgcccgccg cagcacgacc ggatcaaccg gatctccctg aatgccggga tgaacccgta
     9541 tgaattcctc atcactttca cccatgagat cgcccatatg ctgacctggg aaaaatacgg
     9601 ccgaaaagtc ctgcctcacg gttcagcctg gaaagggcaa tatgccgccc tcctggaaga
     9661 acttatcgtg aaagacattt tccccccgga tatccggaaa atcgttgttc accaggtaag
     9721 aaaccccaaa tcgaccagca aatgcgacac gatgctgacg cgggcactcc agccgcacga
     9781 tgaagaaccg cagggcatct tcctggaaga tctccccatg ggcgccagct tccggatgca
     9841 gaacggccgt cgctttatca aacaggacag gctccgcaaa tggtaccgct gcctcagcct
     9901 cgataaccgc aggctttacc gcatcagccc ggtcgcccgt gtcattcctg aagaagataa
     9961 gcattaaatt tttacttttg tctgaataat aattagtcat ttatagtcat ttatagtcat
    10021 ttatagtcat ttatagtcat ttgtagtcat taatgacaat caatgacaac caatgacaac
    10081 caatgacaat caatgacaac caatgacaac caatgacaat caatgacaac caatga
//
LOCUS       DAOPNH010000104        10126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7195_length_10126_cov_5.498262, whole genome shotgun sequence.
ACCESSION   DAOPNH010000104 DAOPNH010000000
VERSION     DAOPNH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_10126_cov_5.498262"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKZ74_06955"
     CDS             complement(<1..495)
                     /locus_tag="PKZ74_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM92535.1"
                     /translation="MKSAEEIYALFQKHPGISTDSRDIRPGTLFFAIRGSHFDGNRFA
                     NDALNNGAAYAIVDDPDSVSDQRYILVDDTLATLQEIAMIHRSGIRAKVIGITGSNGK
                     TTTKELTSRVLSNGYNTLATKGNFNNHIGVPLTVLSITPQTQLAVIEMGANHQKEIEL
                     YCKIA"
     gene            complement(590..2059)
                     /locus_tag="PKZ74_06960"
     CDS             complement(590..2059)
                     /locus_tag="PKZ74_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM92536.1"
                     /translation="MSVIFLAGVLLAFLASCQQQGASRKASRTTGWKYNDPSVGGFEV
                     SSAREQATGPGLVLVEGGRFTMGANFENLTYEWDNIPKTVTVPSFYLDQTEVSNIDYL
                     EYLYWLERVFGEDYPEVVRNARPDTLVWRERLAFNEPLVENYLRHPSYQDYPVVGVNW
                     IQANDYCLWRTDRVNEMILISEGYLDFDPDQKNRDNFNTDAYLAGQYIGVVNKPKANL
                     NPSSTGERSINLQDGILLPKYRLPTEAEWEFAALGLMGNTLYERVVERRIYPWNGNLL
                     RTDQKKYYGSFISNFKRSSGDYMGVAGNLNDGADLPAPVGSYWPNDYGLYNMAGNVAE
                     WVMDVYRPLSPEDVADYAPFRGNVFTKPATDADGFLLEPDSLGRMQRIPVTDEESKDR
                     VNYRKADNINYADGDYQSVSSSDWLTDPGKNTTDEMYEFGATSLITDKSRVIKGGSWK
                     DGPYFLSPGTRRFMDEKLSTNWIGFRCAMVRVGDPMKGR"
     gene            complement(2108..3112)
                     /locus_tag="PKZ74_06965"
     CDS             complement(2108..3112)
                     /locus_tag="PKZ74_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM92537.1"
                     /translation="MLQRILTILTVVTALLFNYKGQAQDPEFSQFYGNPLYLNPALAG
                     AEICPRAVLNYRNQWPGLAKSFVTYNASYDQYFKKLHGGVGVLFNMDNAGNGILKTTQ
                     ASLMYAFSLQASKNLFINMALQGTFYQKSLNWDMLDFYDEIDPQQGFIIPTSEVPPNN
                     NTVIQPDFSAGVVFGWKGILHGGFAAHHLTQPELAFYTENVNKLPMKYTGHLGANINL
                     EGGGMGFAEDVEPTFYIAPNLLYQQQGEFHQLNAGVYVIRLPIIVGAWFRHNFENSDA
                     VIALVGLNYKNLKVGYSYDITLSKLKSNTGGAHEISVAYQFNCIEKKRKIHAIKAPGF
                     "
     gene            3388..7074
                     /gene="porU"
                     /locus_tag="PKZ74_06970"
     CDS             3388..7074
                     /gene="porU"
                     /locus_tag="PKZ74_06970"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPM92538.1"
                     /translation="MPGDESDSVLRAAFGGAKYAKETGGLPIFSAVYPLDGDPALIEA
                     KIANPVYDIAPEGFNLISGIDLLDDVITVSTKKAYDRKMPVACCSFIPARINPYNGEP
                     EILIAFDLVVSAGGSLPDTRQTDGNYIQNSVLATGTWYKLAVTETGIYRISYPDLQNF
                     GINPSGIDPRNIRIYGNGGGMLPESLNEFRHDDLAENRIFVSGESDGKFDPDDYILFY
                     GESPHEWKYQSFSQAFHHFQNIYSDSTYYFLTTGLGPGKRVVSVQSSNEPPDVYISRF
                     TDYDYHEVDLINLTNIGRVWYGEVFDVNTIYEFPFSFPNIDLESQAYFRAYAAAKSES
                     VTSFSFYDNGSKILSSNISGIPPSSNTYARAYIGSNWFTPSSSSLIIKAEFDKNISSS
                     IGWLNFLELNVTRQLIFTGGQMSFREPSAASPGTIAEFALGNAGNAVQIWNVTDPTSA
                     SRIETITSGNGKLFRIAQDTMSEFIAFDGSNYLKPVYAGITGNQNLHGSGPQDMIIIT
                     HESFREQADRLAGFHREHDGLSVLVAGIEEVYNEFSSGAQDITAIRNFMKMLYDVAPA
                     GQEPRYLLLFGDASFDYKDRIQDNSNLVPTWEDEESLVIVYSIASDDYYGFLDGPGDN
                     LLDIGIGRLPVRTVEQATVAVDKIIHYATNTPEVMKEWRNYICFVADDEDGNLHIRQA
                     EEMATFIDTTYGAYNIDKIYIDAFPQVSTPGGQRAPDVNRALNNRIDKGCLVMNYTGH
                     GGEVGWGHERFLENTDINSWTNYDHMPIFITATCEFSRYDDPERISAGEYTFLNPLGG
                     AIAMFTTARATFGGSNFNLNTALFDYMFEKDNGEHYRFGDLIRLAKNKGGVDANDKKF
                     ILLGDPALHLAYPEHEVVTTRINGEDISGVPDTLQALRKITIEGEVRSSGSREEGFNG
                     TVYPIVFDKPSRVTTLAADQTSYQRTFDLQNNILYKGKAQVVDGVFNFTFIVPRDIAY
                     QYGFGKISYYAATENEDAHGFYRNILVGGLDASADPDFAGPEVSLYMNDENFRSGGVT
                     DENPFMLAFVDDVSGINTVGSGIGHDIVAILDENTEKPIILNDFYESDLNSYSSGSIR
                     FPFHGLDEGLHSLSLKVWDIFNNSSEAYIEFNVVRSSNFMIEELSNNPNPFREGTSIV
                     FSHNRAEGNLEFSLDIFNLNGMIVRSFNENIVPTGYRTEAIYWDGRGSDGNALANGIY
                     FCRIIVRNEEGNMASKTAKLILLR"
     gene            7138..>7835
                     /locus_tag="PKZ74_06975"
     CDS             7138..>7835
                     /locus_tag="PKZ74_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92539.1"
                     /translation="MKYFTLRLISIFIAFQAGFFARAQNEVHSVKVELDWQSDLNLFP
                     GHSLLVFEGSVNHDSLGFLPVFHYLMPEHPSRILTDAVIRNEISEPFEIESPVNYPDL
                     EKIGSKPILIIANLASRRSTIPAVYVMPVYRDESGSYRRLTSFTLEVSVSTKNVSNRE
                     TDAREYAAHSVLREGDWFRIAVRQTGVHILTYQDLVDMGFNPGQSDPRKISLFGNGNG
                     MLPEPNSMDRPDDLT"
     assembly_gap    7836..7935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7936..>10126
                     /gene="porU"
                     /locus_tag="PKZ74_06980"
     CDS             <7936..>10126
                     /gene="porU"
                     /locus_tag="PKZ74_06980"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPM92540.1"
                     /translation="NLIKSGKIWYGEVFDQKLDYTFPFHLPGKVNEEPVILKLSLAGR
                     TTSRTNFEVMVSDQLQMELDVPSIVLNSQIYGRPILSGFEPFYTSNDSAVIRIIYNKT
                     GTIDKGWLNYIELNYIRNLEHTGNQLLFRDMRIVGDSVVARYHLSGAAPGTTVWDITD
                     PAMVLNIPVTAESNGVTITTSADSGREFVAFSPGAYYSPEFVEKVENQDLHAMGSADY
                     IIVAHPLFLEQANRLADFHRQYSGMQVHIVTPRQIYNEFSSGAQDVSAIRDFVKMIYD
                     RAGDGEEPRYLLLFGDASYDYKRFLEIDNNFVPAYQSRESLKAAASFVTDDFFGCMDI
                     DEGSNGSGTMDLGVGRFPVHTLEQAKAMVDKTIQYMSPVRENFGPWRNSICFIGDDED
                     NNTHFGQAEGLVKIADSLGPAFNVNKIYLDAYSQLKTPSGNRYPDVNKAIDQVVGEGA
                     LIVNYTGHGGEIAWAEERILDIPAIQSYTNLNSLPTFVTATCEFSRYDDPGLVSAGEL
                     VFLNPEGAGIGLFTTTRLAFSQSNYALNKRFYYESFIIDPLTGEYPRQGDLIRVAKTP
                     SNQNIKNFVLLGDPALMLAYPKMKVSALNVLNERSGRMTDTISAQSKVTIEGQVEDLQ
                     GNRLEGFNGTVHVTVFDKPVLYTTRANDPSSKVADFYMQDKKIFRGEASVANGLFSFS
                     FIVPVDISYQFGDGKISYYALDTNNLIDAQGYDHIIVGGQDELAITDD"
BASE COUNT         2557 a         2263 c         2479 g         2727 t
ORIGIN      
        1 agcaatttta caatacaatt caatttcctt ttgatggttg gcccccattt cgattacggc
       61 tagttgtgtt tggggtgtta tggaaagaac agtcagtggt accccgatgt ggttattgaa
      121 gtttcctttt gttgcgagtg tgttgtatcc gtttgagagc acacgggatg tcagttcttt
      181 ggtggttgtt ttgccgttcg atcccgtaat accgatcact tttgctctaa tccccgacct
      241 gtggatcatg gctatttcct ggagggtcgc cagggtatca tcaacaagga tatatctctg
      301 gtcggaaact gaatccggat cgtcgacgat cgcataagcg gccccgttgt tcaatgcatc
      361 gtttgcaaac cggttaccgt cgaaatgaga accccggata gcgaaaaaca gggttccggg
      421 ccggatgtcc cgtgagtcgg tggaaattcc cgggtgcttt tgaaacaggg cataaatttc
      481 ttcggcagat ttcatagaaa gaaattcata gaaattgaca aagataaaaa aagaggctgt
      541 tcaaagattt taaacagcct ccggatttga agcttcaggc tttaatgttt tatctccctt
      601 tcatggggtc tccgacgcga accatagcac accggaaacc aatccagttg gtggaaagtt
      661 tttcatccat aaatcttctt gttcccgggc tgaggaaata gggaccatct ttccaggaac
      721 cgcctttgat cactctggat ttatcggtga ttagggatgt tgccccaaac tcgtacatct
      781 cgtcggtagt attcttaccc gggtctgtga gccaatccga agaggagacc gactgatagt
      841 cgccatcggc ataattaata ttatcggctt tacggtaatt aacgcggtct ttgctttcct
      901 cgtccgtcac ggggattctt tgcattctgc ccaggctgtc gggttcgagc agaaatccat
      961 cggcatcggt agctggtttg gtaaagacgt taccgcggaa aggggcataa tcggcaacat
     1021 cttccggtga caggggacga tacacatcca taacccattc agccacatta ccggccatgt
     1081 tataaagccc atagtcattt ggccagtaac tgccaacagg tgcgggcagg tccgctccgt
     1141 cgttcaggtt tccggcaacc cccatatagt cgccgctcga ccttttgaag ttggaaataa
     1201 agcttccgta gtattttttc tgatcagtgc ggagaaggtt tccgttccag gggtaaatgc
     1261 gtcgttcgac aaccctctca tataaggtgt ttcccataag ccccagcgct gcaaattccc
     1321 attcggcttc ggttgggagc ctgtatttgg gtaagaggat accgtcctgg agattgatgg
     1381 agcgttctcc tgtgctgcta gggttgaggt ttgctttggg tttgttgacc actccaatgt
     1441 attgccctgc caggtaagca tcggtattga aattatcacg gtttttctga tcggggtcaa
     1501 aatcgagata accttctgaa atgaggatca tttcattgac cctgtcggta cgccacaggc
     1561 agtaatcgtt tgcctggatc cagttaacgc ctacgaccgg gtagtcctga taagacggat
     1621 gcctgaggta gttttccacc agaggttcgt taaaagccag gcgctcacgc cagaccagcg
     1681 tgtcggggcg ggcgttccgg acaacttccg gatagtcttc cccgaagacc cgttccagcc
     1741 agtataggta ttccaggtag tcgatattgc tcacttcggt ctggtccagg taaaatgagg
     1801 gaaccgtaac cgttttgggt atgttatccc attcataggt caggttctca aaattagctc
     1861 ccatggtaaa acggccacct tcgaccaata ccaggcccgg acctgttgcc tgttccctgg
     1921 cggatgaaac ttcgaatcca ccgacagagg gatcattgta tttccatcct gtagttcttg
     1981 aggctttcct tgaggcaccc tgctgctggc aggaggccag gaatgcaagc aagacgcctg
     2041 ccaaaaaaat tacgctcaaa cttttaaatc tgatcatata aggtattcat tgaatttcga
     2101 catataacta aaaaccgggt gcttttattg catgaatctt tcttttcttt tcgatacagt
     2161 tgaactgata ggcaactgag atttcatgcg cccccccggt attgcttttc aatttggaca
     2221 atgtgatgtc gtaactatat cccaccttga gatttttata attcagtccg acgagtgcga
     2281 tgaccgcatc agaattctca aaattgtgcc ggaaccaggc cccgacgatg atcggcaggc
     2341 ggatcacata aaccccggcg ttcaactggt gaaattctcc ctgctgctga tacaacaggt
     2401 taggtgcaat ataaaaggta ggttcgacat cttcagcaaa tcccattcct ccaccttcaa
     2461 ggtttatatt ggctccaagg tgtccggtat atttcattgg aagtttattg acattttcgg
     2521 tataaaatgc cagttccggc tgggtcaggt gatgggccgc aaaacctccg tgcagaatcc
     2581 ctttccaccc gaataccact ccggcggaga agtcgggctg aataacggta ttgttattcg
     2641 gcgggacttc cgaggtggga atgatgaatc cttgctgggg atctatttca tcataaaaat
     2701 ccagcatatc ccagttgagt gatttttggt aaaaggtgcc ctgcaaagcc atgttgatga
     2761 acaaattttt cgaagcctgg aggctgaagg cgtacatcag gctggcctgg gttgttttca
     2821 gaatgccgtt gcccgcatta tccatattga aaagtacgcc gaccccaccg tgcagttttt
     2881 tgaaatactg gtcataggaa gcattatagg tgacgaaact tttggcaagc ccgggccact
     2941 gattcctgta attgagtact gcccgcgggc agatttcggc tcctgccaat gccggattca
     3001 ggtataacgg attgccataa aactgtgaga attcgggatc ctgtgcctgc cccttgtagt
     3061 tgaacaaaag tgctgttaca acagttaata tagttaaaat tctttgcaac atcaatccgg
     3121 ccttaaattt gagggtacaa ttatacaata ttttttaatt caaaaatttt tttttgaatt
     3181 tattacactg acaatatcaa cataaaacgg acgttatatt ttacgtttat gaatctgtgt
     3241 tttcggattg gacggtaagc aattctaact aaattaaatt ccatgaaatc agctgcattg
     3301 ttgctctttt tatttttgac ggcagagatt ttctgtaccg gacaaactgt ttatcagggc
     3361 cgcattaaat ggacggggtt ggaagttgtg cctggcgacg aatccgactc ggtattaagg
     3421 gctgcattcg gcggggcgaa atatgctaag gagacaggtg gcttacccat attttccgct
     3481 gtttatccct tagatgggga tccggcgctg attgaagcaa aaattgcaaa cccggtctat
     3541 gatattgcgc cggaaggttt taatttaatt tcgggaatcg atcttttgga cgatgtgatt
     3601 accgtcagca ctaaaaaagc ttatgacagg aagatgcctg ttgcctgttg ttctttcatc
     3661 ccggccagga ttaatccata taacggagaa ccggagattc tgatcgcttt cgatcttgtt
     3721 gtttcagccg ggggatcatt acctgacaca cgccagacgg acggcaatta tatccagaat
     3781 tcggtccttg cgaccgggac gtggtacaaa ctggctgtta ctgagaccgg tatatacagg
     3841 atatcatatc ctgaccttca gaatttcggt atcaatcctt caggaataga tccccgaaat
     3901 atccggattt atggtaacgg cggtggtatg ctgcccgagt cgctcaacga attcaggcat
     3961 gatgaccttg ctgagaaccg gatattcgta tcgggcgaat cggatggaaa atttgatccc
     4021 gatgattata ttttatttta cggcgaatct ccccatgagt ggaaatacca gtcgttcagc
     4081 caggcgtttc accattttca aaacatatac agcgattcaa cttattattt cttaacaacc
     4141 ggtcttgggc caggaaaaag ggttgtctcc gttcagtctt cgaatgaacc gcctgatgtt
     4201 tatatttcca ggtttacgga ttacgactac catgaggttg atcttatcaa cctgaccaat
     4261 atcggcaggg tatggtatgg tgaggttttc gatgtcaata ccatttatga gtttccgttt
     4321 tcatttccta atattgacct ggaaagccag gcctatttcc gggcctatgc ggcggcaaaa
     4381 tcggaatctg ttacatcttt cagtttttat gataacggaa gtaagatcct cagcagtaac
     4441 atcagcggca ttccgccatc ttccaataca tacgccaggg catatatagg cagcaactgg
     4501 tttacccctt catcgtccag cctgattatc aaagcggaat ttgataaaaa catatccagt
     4561 tcaatcgggt ggctgaattt cctggaattg aacgttaccc gtcaactcat ttttaccggc
     4621 ggacaaatgt cgtttcgtga accctccgcc gcttctccgg gaacaatagc ggagtttgcc
     4681 cttggcaatg ccggtaatgc agtccagatc tggaatgtga ccgatccgac cagtgcttca
     4741 aggattgaaa cgatcacatc cggaaacgga aagttgtttc gcatagccca ggatacaatg
     4801 tccgaattta ttgctttcga cggttcaaat tatcttaagc ctgtgtatgc cggcataacg
     4861 ggcaatcaga accttcatgg ctccggacct caagatatga tcatcatcac gcacgagtcg
     4921 ttcagggagc aggccgacag actggccggc tttcacaggg agcacgatgg cttatcggtt
     4981 ttagtcgctg gaattgaaga agtttacaat gaattttctt cgggtgctca ggatattaca
     5041 gccatccgca actttatgaa aatgctttac gatgttgccc ctgccggcca ggagccccgg
     5101 tatctcctgt tgtttggaga tgcttctttt gattacaagg acaggattca ggataactcc
     5161 aatctggtgc caacttggga agacgaggaa tcgctggtca ttgtatattc tattgcttcg
     5221 gatgattatt acggttttct ggatggcccc ggtgataacc ttcttgatat tggtatcggc
     5281 cggttgccgg ttcgtacggt tgaacaggcc acggtagccg tcgacaagat cattcattat
     5341 gccaccaata cgccggaagt gatgaaggaa tggcggaact acatttgctt tgtcgccgac
     5401 gatgaggacg gtaatttgca tatccggcag gcggaagaga tggcgacctt catcgacacc
     5461 acctacggtg cctataatat tgataaaatc tatatcgatg cttttccaca ggtttcaacg
     5521 cctggtggcc aaagggcgcc agacgtcaac cgcgcattga acaacaggat cgataaaggc
     5581 tgcctggtga tgaactacac agggcacggg ggagaggtcg gctgggggca cgagaggttc
     5641 ctggaaaaca ccgatattaa ttcctggacg aattacgacc atatgcccat cttcattacc
     5701 gctacctgcg agttcagccg ctatgatgat ccggaaagga tttcggccgg ggaatacacc
     5761 tttctgaatc ccctgggagg ggctattgcc atgtttacca ctgcccgggc aacctttggc
     5821 ggttccaact tcaatcttaa tactgctctt tttgactata tgtttgaaaa agataacggg
     5881 gagcattacc gtttcgggga tctgatccgg ctggctaaaa acaagggggg tgtggatgcc
     5941 aacgataaaa aattcatcct tctgggtgat cctgccttgc atctggcata tccggaacat
     6001 gaggtagtga ctacccgtat caacggggag gatatttcgg gcgtgcccga tacgctccag
     6061 gcactgagaa aaataacgat cgaaggggaa gtcaggagtt caggttcaag agaagaggga
     6121 tttaacggga cggtgtatcc gattgtgttc gataaacctt ccagggtgac aaccctggct
     6181 gctgaccaga caagctatca gcgtacgttc gaccttcaga ataacatatt atacaaaggg
     6241 aaagcgcagg tggtcgacgg agttttcaat tttacattca ttgtaccgcg cgacattgcc
     6301 taccagtatg gctttggcaa gataagctac tatgcggcca ccgaaaatga ggatgcccat
     6361 ggtttttacc gcaatatcct ggtcgggggg ctggatgctt cggcagaccc tgactttgcc
     6421 gggccggaag tgagtttata catgaatgat gaaaatttcc ggtcaggagg tgttacagat
     6481 gaaaatccct ttatgctggc tttcgtcgat gatgtcagcg gtataaacac ggtcggatcg
     6541 ggtataggcc atgatattgt ggccattctg gatgaaaata ccgaaaaacc gatcatactc
     6601 aacgattttt acgaatccga cctcaacagc tattccagtg gctcgatccg gtttccattc
     6661 cacggcctgg atgaagggct tcacagtctt tcactgaaag tctgggatat cttcaacaac
     6721 agttcagaag cttatattga gttcaatgtg gtacggtctt ccaattttat gatagaggag
     6781 ttgtctaaca atccgaatcc tttcagggag gggacttcca ttgttttttc acataaccgg
     6841 gctgaaggaa acctggaatt ttcactggat attttcaatc tgaatggaat gattgtcagg
     6901 tcttttaatg aaaatattgt ccctaccggt taccgtacgg aagcgattta ctgggatggc
     6961 cgcggttcag acggcaatgc cctggccaat ggcatttact tttgcaggat tattgtgaga
     7021 aatgaggagg ggaatatggc atcaaaaact gcaaagctga tcctgctccg gtaagatttt
     7081 ttaaacttca ggcaactttt taatgcgtaa gggtgtctta gactatattt gtacaggatg
     7141 aaatatttta ccctcagact catatccatt ttcattgcat tccaggcagg ttttttcgcc
     7201 cgggcgcaaa acgaagttca ttcggtgaaa gtggaattag actggcaatc cgatctcaat
     7261 ttatttcccg gacattccct tttggtattt gaaggttctg tgaaccatga cagtcttgga
     7321 ttcctgccgg tttttcatta cctgatgcct gaacatccca gccggatcct taccgacgca
     7381 gtaatccgga atgaaatatc ggaacctttt gaaattgaaa gcccggtgaa ctatcccgat
     7441 ctggaaaaga tcggttccaa accgatcctg ataattgcca atcttgcatc gaggcggtcg
     7501 acgataccgg ctgtatatgt tatgccggtt taccgtgatg agagcggcag ttaccgccgg
     7561 ttgacctctt tcacacttga agtctcggta agtacaaaaa atgtatccaa tcgtgagaca
     7621 gatgccaggg aatatgctgc tcattcggtt ttgcgcgagg gagattggtt ccggattgct
     7681 gtcagacaaa ccggtgtgca tattcttact taccaggacc ttgttgatat gggatttaat
     7741 cccggccagt cggatccccg caagatatct ttatttggaa acggcaatgg aatgcttccc
     7801 gaacctaatt ccatggacag gccggatgat ttgacnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnaacct gataaaatcg ggtaaaatat ggtatggcga agtctttgac
     7981 cagaaactcg attacacttt tccattccat ttgcccggca aagtaaacga agaacctgtt
     8041 attctgaagc ttagcctggc cggcaggacg acatcacgca caaatttcga ggttatggtt
     8101 tcagaccagc ttcaaatgga acttgatgta ccgtccattg tgctgaattc acagatttac
     8161 ggccggccga tcctgtcggg attcgagccg ttctatacca gtaacgattc tgctgtcatc
     8221 cggataatat acaataaaac gggaacaata gacaaagggt ggctgaatta tatcgaatta
     8281 aattatatcc ggaatcttga acacaccggg aaccagctgc ttttcaggga tatgaggatt
     8341 gtcggcgaca gtgttgtggc ccggtatcat ctttccggag ccgcgcccgg aacaaccgtt
     8401 tgggatatca ctgatccggc gatggtcctg aatattccgg taacagcgga atccaatggg
     8461 gttactataa caacatcagc agattccggg cgggaatttg tcgcattcag cccaggcgcc
     8521 tattattcgc ccgaatttgt tgagaaggtg gaaaaccagg atttgcacgc aatgggtagt
     8581 gcggattaca tcattgtagc tcacccgttg tttctcgaac aggcaaacag gctggccgat
     8641 ttccacaggc aatacagcgg catgcaggtg catatcgtga caccccggca gatttacaat
     8701 gagttttctt caggggctca ggatgtgtcg gccatacggg attttgttaa aatgatatac
     8761 gacagggccg gggatggtga agaaccacgg tacctattgc tgtttggcga tgcttcctat
     8821 gactataaaa gattcctgga gattgacaac aactttgtgc cggcatacca gtcgagggaa
     8881 tcattgaaag ccgccgcttc atttgttaca gacgactttt tcggttgcat ggatattgat
     8941 gaaggcagta acggatcggg gacgatggac ctgggtgtgg gccgtttccc ggtgcacacg
     9001 cttgaacagg cgaaggcgat ggtggataag acgatccaat atatgtcgcc ggtgcgcgag
     9061 aattttggcc cctggcggaa ttccatctgt tttatcgggg atgatgaaga taacaatacc
     9121 catttcggcc aggctgaagg gctggtgaaa attgccgatt cgctgggtcc tgccttcaac
     9181 gtgaacaaga tctacctgga tgcttattcg cagttaaaaa caccgagcgg aaaccgctat
     9241 ccggatgtaa acaaagccat tgaccaggta gtcggtgaag gtgccctcat agtcaattat
     9301 accggacacg gcggggaaat agcctgggcc gaagagagaa ttcttgatat cccagccatt
     9361 caatcataca ccaatctgaa ttcattaccc acatttgtca cggcaacatg cgaattcagc
     9421 cggtatgacg acccggggct ggtttcggcc ggtgagctgg ttttcctcaa tccggaaggc
     9481 gccgggatcg gtttgtttac cacgacgcgc ctggcttttt cacaatccaa ttacgcgctt
     9541 aataagcggt tttattacga atcattcata atcgaccctc tgaccgggga atatcccagg
     9601 cagggcgatc tgatccgtgt agccaaaacg ccttcgaacc agaatatcaa gaattttgtg
     9661 ctcctggggg atccggcatt gatgctggct tatccgaaaa tgaaggtgag tgcgctcaat
     9721 gtgctcaacg agcgatcggg ccggatgaca gacactatca gtgcacaatc gaaagtaacg
     9781 atagaaggcc aggtggaaga tctgcagggc aaccggctgg aaggatttaa cgggaccgtt
     9841 catgtgaccg tatttgacaa accggtgctc tatacgacac gggcgaatga tcccagcagt
     9901 aaagtagccg atttttatat gcaggataaa aagattttca ggggagaggc atctgtggcc
     9961 aacggtcttt tcagtttttc tttcattgta ccggtcgata tttcgtatca gttcggcgat
    10021 ggtaagatca gttattatgc gctggataca aataacctga tagatgccca gggatatgac
    10081 catatcattg tcggtggtca ggacgaactg gccattaccg atgata
//
LOCUS       DAOPNH010000105        10059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7256_length_10059_cov_5.958816, whole genome shotgun sequence.
ACCESSION   DAOPNH010000105 DAOPNH010000000
VERSION     DAOPNH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7256_length_10059_cov_5.958816"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PKZ74_06985"
     CDS             <1..1217
                     /locus_tag="PKZ74_06985"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPM92541.1"
                     /translation="AEVMEAEDTLFILYTSGSTGKPKGVLHTTAGYMVFAQFTFLNVF
                     QYNEGDIYWCTADIGWVTGHTYIVYGPLLSGATSVMFEGVPTYPDPGRFWETVDKYQV
                     NQFYTAPTAIRALIAQGDEWVRSKNLSSLKVLGSVGEPINEEAWRWYHDVVGKGKCPI
                     VDTWWQTETGGILITPLAGITPTKPSFATLPLPGVQPVLVDNEGNVMEGNNVQGNLCI
                     KFPWPGMIRTTYGDHERCRINYFSTYRGLYFTGDGAKRDEEGYYRILGRVDDVINVSG
                     HRIGTAEVEDAINQHPKVVESAVVGYPHEIKGSGIYAYVTCDALSEEEVQTIDKEICD
                     IVSRIIGPIAKPDKIQIVSGLPKTRSGKIMRRILRKIGEGDATSLGDTSTLLDPGVVE
                     EIIRGAKVEVKR"
     gene            1365..2642
                     /locus_tag="PKZ74_06990"
     CDS             1365..2642
                     /locus_tag="PKZ74_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPM92542.1"
                     /translation="MSDIKTMNRWLVVIGAIMIQLALGAIYAWSVYTKTLENSGWSKQ
                     ETQMVFSAGLALFAIVMVVAGRFLQHPKVGPRRLAMASGLVLGLGYVLAGIFGAENFT
                     TTFIFVGIIGGAGIGIGYVVPIAVGIKWFPDKKGLITGLAVAGFGFGATLWMTLAGKL
                     GPLGGGELLKSLGVSGTFIAFGIAYFVLVEIGSIWMKFPPDGFKPAGWNPPQATGSSP
                     VAGSVTLTSAEMLRTPQFYFILLTFAFGASAGLMSIGLMKLWPMEALEANGIAPETAS
                     AMATLAMAVFFALFNGLGRILWGMISDKIGRKLSIIIMTATQGVFVYLFQFMAGNEYT
                     LYLFAMLIGFNFGGNFALFPTLTADTFGTKYVGMNYGWVFLAYAIGGIFGPIMGGKLG
                     DLGNFPLAFTICGILCIVAVITISLVKPVIRKA"
     gene            2766..3941
                     /locus_tag="PKZ74_06995"
     CDS             2766..3941
                     /locus_tag="PKZ74_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92543.1"
                     /translation="MSIEIKEVLTRHDIGEFIRFPFRLYRNNPFWLPPVLIDEKNFHN
                     PRKNKALRVNDCIHYLAYKNGQVAGRIMGIIHHRYNEQIGKKRARFFKFDCMDDPDVG
                     HSLITAVEEWARVKGMEEMIGPFGFSDKDPQGLLISGFDIRAAIVAPYNHPYYIDLIE
                     KEGYVKDVDLYEYIIPIPEKIPDFYQRIYDRVSRNEDLRHVVLKTKKDLKPYIMPVMR
                     LINETFRNIYGSYEFDEEEMKKFASDYMMILDVDFTIIITDKGIPVAFLIAVTDIGPA
                     LQKTGGRVFPFGIFRILKEMKRSDFIVLMIGGIKESHQGTGIDVMMGVKMLQAAKRRG
                     IRHMHSHLELEENYKVRGEMEKMGGEVCKVHRVYRKSLVAYPACEQHFSNIVNNFTK"
     gene            4001..6685
                     /locus_tag="PKZ74_07000"
     CDS             4001..6685
                     /locus_tag="PKZ74_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HPM92544.1"
                     /translation="MAVKTLQKIFRPQRIAIIGVSNDPNSVGGITLKNLVGGGFQGVV
                     YPINPKHEAVMGIPCYPDVKSLPKVPDLAVICTNAKSVPGLLKDCGDAGILGIIIMSA
                     GFKETGPEGKKLEEEVKAIVAKYTGMRVIGPNCLGIIVPGNNMNVSFASGTAKKGHVA
                     FISQSGALCTSALDWAKEENIGFSYFVSIGNTMDVGFGDLIDFFGQDPSTRSIILYVE
                     SISKARQFMTAARAFARKKPIIVYKAGRFPESAAAAASHTGAMASEDAVYDAVFQRAG
                     VARVFDIGDIFNFTELMAQKNIPKGRNLAIITNAGGPGVMATDALISMDGKLTKLSDE
                     TMKKLNDYLPPFWSHGNPVDVLGDAPPERFATATRIVLDDPNVDAVLVILTPQAMTNP
                     TATALEITKLADHTVKPIMAAWLGGESMREGISIFNKHGLSTYDTPEGAIRAFMTLAS
                     YSKNLDALFETPKDFPVSFTLDRDKVRKEFVEKHFTKERILSEDVSKALLTAYGIPTT
                     QPMVAKSAIEAADIANRIGYPVVMKIHSPDITHKSDMGGVILNIKDEDGVWNNFKNMT
                     RSIKEKMPSANIEGVTIQPMVNMKDATEMILGIKKDPVFGTVLLVGMGGTGAEVFKDT
                     KLAFPPLNERLTRTMIKSLKIYPFLKGYRGAPEKDIEKLIEVIIRLSYLAADYPEIVE
                     LDINPLLVGTSDVIALDARIVIDKELVGKDLPQFSHLLIHPYPDKYTKPAKLIDGNTI
                     LLRPIKPEDEPLWLDLLGSCSKESIYSRFRYNFHYDSHEIATQFCFIDYAREIAIVAE
                     VMEEGQKKLIGVGRLIADLDHETVEYAVLIADAWQKKELGTILTEYCIEIARQWHLKR
                     IAAETTKDNQAMISVFKKLGFKVVFNQDTGVSVSMDLV"
     gene            complement(6682..7257)
                     /locus_tag="PKZ74_07005"
     CDS             complement(6682..7257)
                     /locus_tag="PKZ74_07005"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPM92545.1"
                     /translation="MFNLILFGPPGSGKGTQSVKIAEKYQLVHISTGDIFRREIKNQT
                     PLGIKVQSIIEKGELVPDELLVDILRSALHQAGSPAGFAFDGFPRTLRQAGDLDNLLA
                     ETHENVSLVLALEVDEEEVVKRLLKRARLEGRKDDTEDVIRNRMSVYHSQTHPLIDFY
                     EKQGKFVAIKGVGSVEEIFADICRAIDKVKT"
     gene            7385..8206
                     /locus_tag="PKZ74_07010"
     CDS             7385..8206
                     /locus_tag="PKZ74_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPM92546.1"
                     /translation="MTDPFTDNSIYSPGRSVCEYLDPGLWEACHASPSATVPEPFLYT
                     AKKNMIFQNLDIALNGSNWIFWILLAGFIALTFTRFYHERRLKLLAKSLFKHSAALQL
                     IRESPLHTHRSFLLFFLIYIISTSLLISQVSVIFSSGDNRTIGSLLFSLTAVGAYIGF
                     ILLKFLLIWIIGIIFKNIETAKEYVQNIIIYNFVQGMLLLPFLALIIYVHPTVFIYIS
                     IGIVLIMIVLRFTRGMMIGLSDPKFTLFHLFLYLCTLEILPLAFAAKWISKYFFS"
     gene            8265..9014
                     /locus_tag="PKZ74_07015"
     CDS             8265..9014
                     /locus_tag="PKZ74_07015"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPM92547.1"
                     /translation="MRILVSQPQPADIEKSPYGDLTKKFNVDIEFFKFIKIEGIPSRE
                     FRQARVNLLDYSAVIFTSRNAVDHYFRMAKELRADIPQTMKYFCMSESTAFYLQKYVQ
                     FRKRKIFHGKQNFEELMEIIKKHKEEKYLLPCSDIHKASISGMLDEGEVNYTKAIFYR
                     TLASDLSHIDIKKYDMLIFFSPSGIASLMKNFPEFRQNSTIIGAFGPTTSQAVKDAGL
                     TLNIEAPTKTAPSMTMAIEEFLQKQTKKNHK"
     gene            9090..9512
                     /gene="rnpA"
                     /locus_tag="PKZ74_07020"
     CDS             9090..9512
                     /gene="rnpA"
                     /locus_tag="PKZ74_07020"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPM92548.1"
                     /translation="MSLFSFRKEEIIRKKKQIDRLFDEGRAFFIHPFKVIYLPYPAEP
                     DSPAQILISVSKRSFKNATDRNRIKRQIREVYRLNKHEFYHYLNEKQTGCLLAVIYSG
                     QVMHETHFLEKKIKAVINRLINELSKNLNNPSISLSDQ"
     gene            9523..>10059
                     /locus_tag="PKZ74_07025"
     CDS             9523..>10059
                     /locus_tag="PKZ74_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPM92549.1"
                     /translation="MNSIRKSLVIFLSLLAFLPVISLSQAENNFEVSKNLDIYATLLK
                     ELDKNYAEAINPGELTQAAIDAMLETLDPYTVYIPESNAEDYKLMTTGQYGGIGALIH
                     KSGSYVVISEPYEGFPAQKAGLRAGDKILEINGKTMKDKNSDEVSEILKGQPGVELEL
                     LIGRINVPDPVRMKVVREE"
BASE COUNT         2714 a         2426 c         2415 g         2504 t
ORIGIN      
        1 aggccgaagt catggaagcc gaggatacgc tgtttatcct ttatacctcc ggatcaacag
       61 gcaaaccgaa aggcgttctc cataccacag ccggatacat ggtatttgcc cagttcacgt
      121 tcctgaatgt tttccagtac aatgaaggcg atatttactg gtgtacagcc gatatcggct
      181 gggtaacggg ccatacctat atcgtttacg ggccgttgct ttcgggcgcc acatcggtca
      241 tgttcgaagg agtacctacc tatcccgatc cgggcaggtt ctgggaaacg gtcgacaaat
      301 accaggtcaa ccagttctat actgcgccga cggctatccg cgccctgatc gcacagggag
      361 acgagtgggt gcgttccaaa aacctcagtt cactcaaagt cctcggttcg gttggcgaac
      421 caatcaacga agaggcatgg cgctggtatc acgatgtggt gggcaaaggc aaatgcccga
      481 tcgtcgacac ctggtggcag acggaaaccg gtggcatcct gatcacgccg ctggccggca
      541 ttaccccgac caaaccgtcg tttgccacac tgccattgcc cggggtacaa ccggtgctgg
      601 tcgataacga aggcaatgtc atggagggga ataatgtaca gggtaacctt tgcatcaagt
      661 tcccgtggcc gggcatgatc cgtacgacct atggcgacca tgagcgctgc cggatcaact
      721 atttctcgac ctaccgcggg ctctatttca ccggcgacgg cgccaaacgc gacgaggaag
      781 gctactaccg catcctgggc cgtgtcgacg acgttatcaa tgtttcgggc caccgcatcg
      841 gaacggccga agtggaagac gccatcaacc agcacccgaa ggtggtggaa tcggctgtcg
      901 ttggctatcc tcatgaaata aagggttcag gcatctatgc ctatgtaacc tgtgatgcgc
      961 tttcggaaga ggaagtccag accatcgaca aagagatatg cgacatcgtc agccggatca
     1021 tcgggccaat cgccaaaccg gacaagatcc agatcgtgag cgggcttccc aagacgcgtt
     1081 cagggaagat catgcgccgt atcctccgca agatcgggga aggtgatgcc accagcctcg
     1141 gcgatacctc taccctgctc gatccgggtg ttgtggaaga aatcatccgc ggcgccaagg
     1201 ttgaagtgaa acggtaatct atcggttggt taaccactaa ggacacaaag gatatcacaa
     1261 aggaacacaa agtacggttt acaattggaa aacttagtgc ttctcagtgc cttggtgcct
     1321 tagtggttat catttaaaaa tcataaccaa aaaccaaaat ccttatgtca gatattaaaa
     1381 caatgaaccg ctggctggtg gttatcgggg ctattatgat tcaacttgcc ctgggtgcta
     1441 tttatgcctg gtcggtttac acgaaaacgc tggaaaattc cggatggagc aagcaggaaa
     1501 cacagatggt cttttcggcc ggactggctt tgtttgccat tgtcatggta gtagccggcc
     1561 ggttcctgca acatccgaaa gtggggcctc gcaggctggc tatggccagc ggactcgtac
     1621 tcggcctggg atatgtgctg gcaggaatat tcggcgcaga aaactttacc acaaccttta
     1681 tttttgtcgg aattatcggt ggagccggga tcgggatcgg gtatgtcgtt cctattgcgg
     1741 taggcattaa atggtttccc gacaagaaag ggctaattac gggtctggct gtcgccggat
     1801 tcggattcgg cgccacactc tggatgacac tggccggcaa actcggtccg ctcgggggtg
     1861 gtgaactgct taaaagcctg ggcgtttcag ggacatttat tgctttcggt attgcctatt
     1921 tcgtgctcgt cgagatcggc agtatctgga tgaaatttcc tccggacgga ttcaagcctg
     1981 ccgggtggaa ccctccgcaa gccacaggat cgtcccctgt cgccggttcg gtcaccctga
     2041 cctcagcaga aatgctgcga acacctcagt tttatttcat cctgctgacc tttgcttttg
     2101 gagccagcgc cggcctgatg agcatcggcc tgatgaagct atggccaatg gaagcactgg
     2161 aggcaaatgg aatcgcacct gaaacagcca gcgctatggc aacactggcc atggccgtat
     2221 tttttgccct tttcaatggc ctcggccgaa tcctgtgggg catgatatcc gataaaatag
     2281 gccggaaact gtctattatt attatgacag caacccaggg cgtatttgtc tatcttttcc
     2341 agtttatggc tggtaatgaa tacactcttt acctatttgc catgttgatc ggctttaatt
     2401 tcggcggcaa cttcgccctt ttccccacat taacagccga cactttcggc accaagtatg
     2461 ttggcatgaa ttatggctgg gtattcctgg cctacgccat cggtgggatc ttcgggccga
     2521 taatgggtgg aaaactgggc gatttgggta actttccact cgcatttact atttgcggaa
     2581 tactctgcat cgttgctgta atcaccattt cactcgtcaa gcctgtcatc aggaaagcct
     2641 gagcctgaat caggattttc atcaccgctg gggtgcaatc gtgcaaagaa ttaaaattta
     2701 actttgccaa tttgttcctc agcggtttta tttttatcag gttcaccagg attaaaaata
     2761 atgatatgtc catagaaata aaagaagttt taacccgcca cgacattggt gaatttatcc
     2821 gattcccctt ccgtctatac aggaacaacc ccttctggct ccctcctgtc ctgatcgacg
     2881 aaaagaattt tcacaacccc agaaaaaaca aagcactgcg cgtaaacgat tgcattcatt
     2941 acctggcgta taaaaacgga caggtggcgg gcaggatcat ggggattatt catcaccggt
     3001 ataatgaaca gatcggaaaa aaaagagcca ggttctttaa attcgattgt atggatgacc
     3061 ctgatgtcgg ccatagtttg atcaccgcag ttgaagaatg ggcacgggtc aagggcatgg
     3121 aagagatgat cgggcccttc ggtttttcgg ataaagatcc acagggattg ctcatcagcg
     3181 ggtttgatat ccgtgcagcc atcgtcgccc cgtataatca tccgtattat atcgatctga
     3241 ttgaaaaaga aggctatgtg aaagatgttg atctctatga atatattatt cccatcccgg
     3301 aaaagatccc cgatttttac cagcgcattt acgaccgggt aagccggaat gaggatctcc
     3361 gccatgtcgt cctgaaaaca aaaaaagatc tcaaacctta tatcatgccg gtcatgcggc
     3421 tgatcaatga aactttccgg aacatatacg gttcatatga gttcgatgaa gaagaaatga
     3481 agaaattcgc ctccgattat atgatgatcc tggatgtgga tttcaccata ataattacag
     3541 ataagggcat tccggtcgcg tttctcatcg ccgttaccga tatcggccct gcccttcaga
     3601 aaaccggcgg cagagttttc ccttttggta ttttccggat tttaaaagag atgaaaagat
     3661 ccgatttcat tgtcctgatg atcggaggaa taaaagaaag ccaccagggc acagggatcg
     3721 atgtgatgat gggcgttaaa atgctccagg cagccaaacg aagaggaatc cggcatatgc
     3781 acagccacct cgaactggaa gaaaactata aggtccgcgg ggaaatggag aaaatgggcg
     3841 gggaagtttg caaggtgcac cgggtgtaca ggaagtcgtt ggtcgcttat ccagcatgcg
     3901 aacaacattt ttccaatatt gttaataatt tcacaaaata aatctacatt tgtgcgttgt
     3961 aaaaaaaagg cttaatataa aaatataaat atataaaaca atggcagtaa agactttaca
     4021 gaagatattc aggccacagc gcatcgcgat catcggcgta tcgaatgatc cgaacagtgt
     4081 tggaggaatt acattaaaaa accttgtagg aggaggcttt cagggggtgg tttacccgat
     4141 taacccgaaa cacgaagcgg tcatggggat tccgtgctac cctgatgtga aaagccttcc
     4201 gaaagtacct gacctggccg tcatctgtac caatgccaaa tcggtcccgg gattactgaa
     4261 agattgcgga gacgcaggta tcctggggat tatcatcatg tcggcaggat ttaaagaaac
     4321 cggtcctgaa ggaaagaagc tggaagaaga ggtcaaagct atcgtggcaa aatataccgg
     4381 gatgagggta atcgggccga actgcctggg aatcattgtt ccgggcaata acatgaacgt
     4441 cagtttcgcc agcgggaccg ctaaaaaagg gcatgtggca tttatatccc agtcgggagc
     4501 cctttgcacc tcggcgcttg actgggccaa ggaagaaaat atcggcttct cctactttgt
     4561 atccataggc aacaccatgg atgtcggttt cggcgacctg atcgactttt tcggacagga
     4621 cccgagcaca cgctccatca tactttatgt cgaatccatc tccaaagcca ggcaattcat
     4681 gacggcagcc cgtgcttttg cccgcaaaaa gccgatcatc gtatataaag ccggaaggtt
     4741 ccccgaatcg gccgccgctg ccgcttccca caccggagcg atggcttcgg aagatgcggt
     4801 ttacgacgct gtctttcaac gtgctggtgt agcccgtgtg ttcgacatcg gcgatatctt
     4861 caatttcacc gaactgatgg cacagaaaaa catcccgaaa gggagaaacc tggccatcat
     4921 aaccaatgcc ggcggtccgg gcgttatggc aaccgatgcg ctcatctcca tggatgggaa
     4981 actgaccaaa ctttcggatg agaccatgaa gaaactgaac gattacctgc cgccattctg
     5041 gtcgcacggc aacccggtcg acgtgctggg cgacgcacca ccggaacgat ttgccacagc
     5101 tacacgcatt gtcctggatg atcccaatgt agacgccgtc ctggtcatcc tcaccccgca
     5161 agccatgacc aatccaaccg ctacagcgct ggagatcacc aagctggccg accacaccgt
     5221 gaaaccgatc atggcggcct ggctcggcgg agaaagcatg cgggaaggga tcagcatctt
     5281 caacaaacac ggattaagca catacgatac ccccgaaggc gccatccgcg catttatgac
     5341 actggcaagc tattcgaaaa acctggatgc cctgtttgaa acaccgaagg atttccctgt
     5401 ttcgtttacc cttgaccggg acaaggtgag gaaagaattt gtcgaaaagc atttcaccaa
     5461 agagagaatc ctgtcggaag acgtttcaaa agcgctcctg acggcctatg gcatacccac
     5521 cactcagccc atggtcgcca aatcggctat tgaagcggcc gatatcgcca accggatcgg
     5581 ctatcccgtt gtcatgaaaa ttcactcacc cgatattaca cataaatcgg atatgggtgg
     5641 cgtcatcctg aatatcaagg acgaagacgg cgtttggaac aacttcaaga atatgacccg
     5701 aagcataaag gaaaagatgc cctcggccaa tatcgaaggg gttaccatcc agccgatggt
     5761 caatatgaag gatgccacgg aaatgatcct cgggatcaag aaagatccgg tttttggtac
     5821 cgtcctcctg gtagggatgg gcggcaccgg cgcggaagtc ttcaaagata ccaagctggc
     5881 atttccgcca cttaatgaac ggcttacgcg caccatgatc aaatcgctga aaatatatcc
     5941 tttcctgaaa ggataccgtg gcgcaccgga aaaagacatc gaaaaactga tcgaagtgat
     6001 catccggtta tcttatctgg ccgccgatta tcctgagatc gtcgagctcg atatcaaccc
     6061 gttgctggta ggcaccagcg atgtcattgc actcgacgcc cgcatcgtca tcgataaaga
     6121 gctcgtcggc aaagaccttc cccagttctc gcacctgctt attcacccct atccggataa
     6181 atataccaaa ccggcaaaac tgatcgacgg caacaccatt ctcttacggc cgatcaaacc
     6241 ggaagacgaa ccgctgtggc tcgatctgct gggtagctgc tccaaggagt cgatctattc
     6301 aaggttccgt tataatttcc attatgattc ccatgaaatt gccacccagt tctgctttat
     6361 agattatgca agggagattg cgatcgtggc ggaagtaatg gaagaagggc agaaaaagtt
     6421 gatcggagtc ggccgcctga tagctgatct tgaccatgaa acggttgaat atgccgtact
     6481 gatcgctgat gcctggcaga agaaagaatt gggaaccatc ctgacggagt attgcattga
     6541 gattgcccgt caatggcatc tgaaacgaat cgccgctgaa acgacaaaag ataaccaggc
     6601 catgatctca gtattcaaaa aactgggatt caaggtagtc tttaaccagg ataccggtgt
     6661 aagtgtatcg atggacctgg tttaagtctt caccttatcg atggcccggc agatatcggc
     6721 aaaaatttct tcgacagagc caaccccttt tatagcgaca aacttgccct gcttttcgta
     6781 gaaatcgatt agcgggtgag tttgagaatg gtagaccgac atcctgttcc ggatcacatc
     6841 ttccgtatcg tctttgcggc cttcaagccg ggcccgtttc aacagccttt tgacgacttc
     6901 ttcctcatcg acttccagag ccaggacgag ggagacgttt tcatgggttt cagcgagcag
     6961 gttatccagg tcgccggcct ggcgcaaggt acgcgggaat ccatcgaagg caaagccggc
     7021 aggcgaacct gcctgatgca aagccgaacg gaggatatcc accagcagtt catcgggaac
     7081 cagttcccct ttttcaatga tgctctgcac tttgatacca agtggggttt gatttttgat
     7141 ctcccggcga aagatatcac ccgtcgagat atgaaccaat tggtactttt cagcgatttt
     7201 caccgactgg gttcctttgc ccgaacccgg aggaccgaat agaatcagat tgaacatatt
     7261 attagtcgtt agccttttac tgattttttg cgaaagtggc ggttgggtaa ccgcgtttac
     7321 aaatatagtg aagcgttttt attcctgcca ttgttttggt taattttgcc caaaatacca
     7381 attcttgaca gaccccttca cagataattc tatttattca cccggcagat cggtctgcga
     7441 atatcttgat ccgggattat gggaggcgtg ccatgcttcg ccgtcagcaa cagttccgga
     7501 gccatttctc tacacggcta aaaagaatat gatctttcag aacctggata ttgctttaaa
     7561 cggaagcaac tggatattct ggatattact ggccgggttt atcgccctga catttacccg
     7621 gttctatcac gaaaggcgct taaaactcct ggctaaatct ttattcaaac attccgcagc
     7681 ccttcaattg atccgtgaga gccccctgca tacgcaccgc agttttctat tgtttttcct
     7741 gatttacatc atttccacca gcttactgat cagccaggtt tctgtcatat tctcctcggg
     7801 cgacaaccgc actattgggt cgctcttgtt ttcgttaaca gccgtggggg cttacatcgg
     7861 ctttattctt ctgaagtttt tgctcatctg gataatcggg attatattta agaacatcga
     7921 aacggcaaag gaatatgttc agaatatcat catttataat tttgtacagg gaatgctgtt
     7981 acttccgttt cttgccctga tcatctatgt ccatccgaca gtttttattt atatcagcat
     8041 cggtatagtg cttataatga ttgttttaag attcaccaga ggaatgatga tcggtttatc
     8101 ggatcctaag tttacgttgt ttcatttatt tttgtacctt tgcaccctcg aaattttgcc
     8161 gcttgctttt gctgcaaaat ggataagcaa atattttttc tcctgagaaa aaatggttgc
     8221 cgttgatgga tttttaatta acgaactgat actttaagaa ataaatgcga atcctggtat
     8281 cacaaccgca gcctgccgac attgagaaat caccatacgg cgacctgacc aagaagttca
     8341 acgttgacat tgaatttttc aagttcatca agattgaagg tatcccctcc agggaatttc
     8401 gccaggcaag ggtcaacctg ctcgattact cagctgttat ctttaccagc cgtaatgccg
     8461 tagatcatta ttttcgtatg gcaaaagagc tcagagcgga tatcccgcag acaatgaagt
     8521 atttctgtat gagcgagtca actgcgtttt atcttcagaa atatgttcag ttccgcaaaa
     8581 ggaaaatctt ccatggcaag cagaattttg aggaactcat ggaaatcatt aaaaaacata
     8641 aagaggaaaa gtatctcctg ccctgctccg acatacacaa ggccagtatc tccggtatgc
     8701 tcgatgaagg tgaggtcaac tacaccaaag ctatttttta ccggaccctg gccagcgact
     8761 tatcgcatat cgatattaaa aaatatgaca tgctgatttt cttcagcccg tcgggtattg
     8821 catcgctgat gaaaaacttt cctgaattca ggcagaacag taccatcatc ggcgcattcg
     8881 ggcctaccac cagccaggct gtcaaggatg ccggattgac gctgaacatc gaagcgccga
     8941 ccaagaccgc cccgtcgatg accatggcaa ttgaagaatt tcttcagaaa caaacaaaga
     9001 agaatcataa ataaccgtcg tgccgttcat cgggaggtaa actgcctcac ctcccctgaa
     9061 ttgcgttacc gttaaatttc atcctgctta tgtcgctttt ctctttcaga aaagaggaga
     9121 tcatccgcaa gaaaaagcaa atagatcggc tatttgacga agggagggct ttttttatac
     9181 atccttttaa agtaatctat ctgccttatc cggcagaacc ggatagcccg gcccaaatac
     9241 tgatcagcgt cagcaaaagg tcgtttaaaa atgccactga ccggaaccgg atcaaaaggc
     9301 agatccggga agtataccga ctcaataaac atgaatttta tcactatctg aatgaaaaac
     9361 agaccggatg ccttttggct gttatttatt caggacaggt catgcacgaa actcattttc
     9421 ttgagaaaaa aataaaagcc gtaataaacc gtttaatcaa tgaactttca aaaaatctga
     9481 acaatccttc catatctttg tctgatcaat aaatatgcag ttatgaacag tattcgtaaa
     9541 agcctggtaa tattcctgtc gctgctggct tttttaccgg ttatttctct tagccaggca
     9601 gagaacaatt tcgaagtatc caagaacctg gatatttatg caactttact gaaagagctt
     9661 gacaagaatt atgcagaggc gataaatcct ggcgaactga cacaggcagc catcgatgcc
     9721 atgttggaaa cccttgatcc ttacacggta tatattccgg aatcgaatgc cgaagattat
     9781 aaactgatga ctaccgggca atatggcggc atcggcgctt tgatccataa aagcggcagt
     9841 tatgtcgtca tttcggagcc ttatgaagga ttccctgccc agaaggccgg gttgcgcgct
     9901 ggagacaaga tcctggagat caatggcaaa acaatgaagg ataagaactc cgatgaggtc
     9961 agcgaaattt taaaagggca gcccggcgtg gaactggaat tactcattgg ccggattaat
    10021 gtaccggatc cggtcaggat gaaggttgtc agggaagag
//
LOCUS       DAOPNH010000106        10050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7262_length_10050_cov_7.061331, whole genome shotgun sequence.
ACCESSION   DAOPNH010000106 DAOPNH010000000
VERSION     DAOPNH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_10050_cov_7.061331"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKZ74_07030"
     CDS             <1..981
                     /locus_tag="PKZ74_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM92550.1"
                     /translation="SKTVSVLIPARNEEENIGRLLDDLSASEYPHLRIFVYDDESEDR
                     TADIVIEKSRMDDRIILIRGRIPGPGWLGKNHGCSRLAEAAVGDYFLFIDADVRVSKT
                     LINSAVQSMEQHQLALLSIFPVQMMNSTGEWLTVPLVNRILVANLPLCLVNRSYIQAF
                     SAANGQFMMFKADVYRLHRFHERFSDEKVEDIRIIRFMKSLRLPVRTLLSDGQIKCRM
                     YRGFGDSMNGFAKNIHAFFGRNWLILFVYVILTTTGPFATWNTFGFTGLIFYLAGLIL
                     FSILVSLQSKQPVWRNLLLMPFQQVIVVMIAFLAAFRHSTGTLKWKGRRV"
     gene            1047..2243
                     /locus_tag="PKZ74_07035"
     CDS             1047..2243
                     /locus_tag="PKZ74_07035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM92551.1"
                     /translation="MTSKASVILVPGRESSVLRRHPWIFSGAVRNVQGNPSAGETVEI
                     YSSAGQWLARGSYSPGSQIRIRILTFDAGETVDETFFENRIRRAVELRQSLPGFREGN
                     CARLIFSESDSLPGLIVDKYDDFLVCQFLSAGAEFWKDIITPKLQEIIPCRGIFERSD
                     SDSRAKEGFAERTGLISGEEPPARVAVDDGKMKFWVDIRQGHKTGFYLDQRLNRLKIM
                     DYANGREVLNAFSYTGGFGVAAAIAGASTVVNVDSSKDALELATENFRLNGLALEKSD
                     FAEDDVFQLLREYRDRRRQFDMIVLDPPKFVASASQLDKGTRGYKDINLLAFKLLRPG
                     GILVTFSCSGYVKPDLFAKIVSDAALDAGRDAVIVENLHQSPDHPVALNFPEGLYLKG
                     LICRVS"
     gene            complement(2263..3099)
                     /locus_tag="PKZ74_07040"
     CDS             complement(2263..3099)
                     /locus_tag="PKZ74_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92552.1"
                     /translation="MHSDHLPGNGKIFRNLLFRISSYTLTAYFLSFIAEKLLVITGAF
                     LFDFKITWLYNKLIIKGADSNWTQENVLIIYLMPLVIMAIIIVLIYLPKKEKDPQPHY
                     KDLFLTWFAFFLVYRILGMTPFNLVVRTNVFFVFDWLYAGPLVLFSAGILSFILYLLI
                     TNKLLKDYYLHAALLSRNHSRLGNHDFMLASILVPLFITSIAATLFFLPGPPYEELAG
                     LVMMNLPAVMIFIRYKLDPPVLLMTKKETSEMTFASWIFPVCLLTVLAFRIILALNEI
                     IA"
     gene            complement(3107..3520)
                     /gene="mscL"
                     /locus_tag="PKZ74_07045"
     CDS             complement(3107..3520)
                     /gene="mscL"
                     /locus_tag="PKZ74_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPM92553.1"
                     /translation="MGLLKEFKEFAMKGNVIDLAVAVIIGGAFGKIVTSFVNDILMPP
                     LGLLLGGVDFKDLKLIIKEGAEGVAAVTWNYGMFIQNIIDFLIIAFAIFMAIRSMTKM
                     QQLRAKPAEPEPEPAPPEPSNEEKLLTEIRDLLKK"
     gene            complement(3603..4700)
                     /gene="corA"
                     /locus_tag="PKZ74_07050"
     CDS             complement(3603..4700)
                     /gene="corA"
                     /locus_tag="PKZ74_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPM92554.1"
                     /translation="MKSRKKTNHSPRNKIPLKDTRKSGKTGLPPGSLVHIGKKIADSV
                     TVRHWAFNSSEFSSTAVIDLHELKTSEGVNNWFEICGLHDTTLMDRLGKALNISPLVL
                     EDILNTEHRPKFDIQGGMLFLTLKAFYISGEGEILDEQASFILGSGYLVSFHERSHSW
                     FDSIKAAMEAGNTRIRQRGNDFILYSLLDIIIDRYYAVAESLGDRLEELEELIFENPD
                     RTLIDKNREIKKEIIKLRKALIPSLEAINKLKRGESDLVMKDTLPYFDDIYDHMVQVL
                     DYIDTYREISAELKESYLSNLTLRMNQVMKLLTIITSIFIPLSFIAGIYGMNFRNMPE
                     LGWKYGYYTILGIMFLLTVGMLIYFRRKKWL"
     gene            complement(4741..5136)
                     /locus_tag="PKZ74_07055"
     CDS             complement(4741..5136)
                     /locus_tag="PKZ74_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92555.1"
                     /translation="MDQKKLEKLENIKNYLENDDISGLNKYIERIKPMVKDDEDFQEI
                     FEELKGKNYDQALFLAEEFIYETDEEADTYGDDFDKVDLEDFDGKNDLMLEGPGDDLE
                     EFDEDLNDDFSEDQYDDLAYYEDKDDDYY"
     gene            5306..7378
                     /locus_tag="PKZ74_07060"
     CDS             5306..7378
                     /locus_tag="PKZ74_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPM92556.1"
                     /translation="MSFLYPYMLWGLAAVSIPVIIHLFNFRQFRKVYFTNVRFLDELK
                     QQTRRQSQLRHLLVMISRMLAIASLVLAFARPYIPVEKEMTPPQAVNQVSIYIDNSFS
                     MEALSSAGPLLEVARNAAREIAAGYKSTDRFMLTVNEFEGRHQRWVNRDEFLQMIGEI
                     VPTPAVRILSEVIRRQQNSLTGSHGESVTSYLISDFQTSMADLENVKPDSLNPLFLVP
                     LQAVRSENLYIDSCWFVSPVHQIDQGVKLVARVVNDSRTDYEKVPLKLSVNGLQKAVA
                     SFDITAGTNYDVEIPFTNHEPGIQLGIVEITDYPVVFDDQMFIVYNVAGSIPVLAING
                     NQESPFLNSLFGKDPEFGFVNNPLGNIDYNRLREFELIILNEVENLSTGLIQELTIYL
                     ENGGSLLIIPSEKLDIGSYTDFLQSVNVNFYGEFTRELTRVTEIDRDNPVFADVFERN
                     VASERGGNTMDLPEVRSFFPVTRNARTAQVPVMTMQNGKPFLTMEKAGKGLVYLLAVP
                     LAESSGNFARHAIFVPALYRIALLSAASDPLYFVIGRDNRVSMSNPMLSGDYVLRIVS
                     SGGDYEFIPGMQTQNNLFNILINNQIRSAGHYFVRKGEEDIKGLGFNYDRNESVMEFS
                     GAEELNRMIDKYGFSRMRVISEDGKPLAESVKDMNQGTVLWRLFIILALVFLAFEIVL
                     LRYWKLKS"
     gene            7375..7917
                     /locus_tag="PKZ74_07065"
     CDS             7375..7917
                     /locus_tag="PKZ74_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPM92557.1"
                     /translation="MRILREQSAGLVIDIQEKLFPFISGNDALTSSSVILVKGLQALK
                     IPIITTVQYTKGLGPTIRPLRELLPDEETIEKLSFSCCDDPVFMKKLESIQKRFIIIS
                     GIESHVCVLQTVTDLLEKGYVPVVAEDCVSSRKLNDKNMAIARMHRQGAIITTYESVL
                     FELLRYSGTEEFKAISRLVK"
     gene            7935..>10050
                     /locus_tag="PKZ74_07070"
     CDS             7935..>10050
                     /locus_tag="PKZ74_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPM92558.1"
                     /translation="MDEIREDMTEGREEPGNLNSEQSPGADTHRTIKISGLYENWFLD
                     YASYVILERAVPEVIDGLKPVQRRILHSMKQLDDGRFNKVANIIGHTMQYHPHGDASI
                     GDALVQLGQKDLLIETQGNWGNILTGDSAAAPRYIEARLSKFALEVVFNPKTTPWKPS
                     YDGRNKEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFIELIDASINILKEEEFEIY
                     PDFQTGGLIDVSRYNDGARGGKVRIRAKIKKIDNKTLVIQEIPFGVTTTNLIDSIIQA
                     NDKKKIKIRKIDDNTAAEAEILVHLVPGTSPDQTIDALYAFTLCEVPVSPNACVIRDG
                     KPQFLDVREILRISTRNTVGLIRRELEIRLDELREQWHYASLEKIFIEHKIYRKIETC
                     ETWEEVIETIDKGLAPFTKKLVRPVTHEDIVRLTEIRIKRISKFDSNKADDSILSLEN
                     EMEEVQNHLDHLIDYAILYFEQIRKKYSAGRERKTEIRSFDTIEAAAVAAASQKLYFN
                     REEGFAGTSLKKDEYICDCSDIDDVILFREDGTFLVRKVEGKFFTGKDVVYINVFKKN
                     DERTIYNMVYRDGKKGSCYVKRFAVVGVTRDKEYDLTKGNPGSKVVYFSANPNGEAEM
                     IRIFLRPKPRMKKTTFDFDFSSIAIKGRSSQGNLLSKNPVRQIVKKEHGVSTLGARGI
                     WYDESVKRLNAEERGIFLGAFEG"
BASE COUNT         2639 a         2289 c         2497 g         2625 t
ORIGIN      
        1 tccaaaaccg tttcggtgct cattcctgcc cggaacgagg aagagaacat tggcaggctg
       61 ctggatgacc tttctgcctc cgagtatcct catctcagga tctttgttta tgatgatgaa
      121 agtgaagata gaacagcgga tattgttatt gaaaaaagcc ggatggatga ccggatcatc
      181 ctgatcaggg gaagaattcc ggggcccggc tggctcggaa aaaatcatgg ctgttcccgg
      241 ctggcagaag cagcagtagg agattatttt ttgtttattg atgccgatgt tcgtgtcagt
      301 aaaacgctga tcaacagtgc cgtacaaagt atggaacaac atcagctggc tttgttgtcg
      361 atatttccgg tgcagatgat gaacagtacc ggggaatggt taacggtgcc gttggtgaac
      421 aggatactgg tcgctaacct ccctctttgc ctggtaaaca gaagttatat acaggctttt
      481 tcggctgcga acgggcaatt tatgatgttc aaagcggatg tttaccgcct gcacaggttt
      541 catgaacgat tcagtgacga aaaggtcgaa gatatcagga tcatccgttt tatgaaaagc
      601 cttcgcctgc ctgtccgtac tttgctcagc gacgggcaga taaaatgcag gatgtaccgc
      661 ggattcggag attcgatgaa tgggtttgcc aagaatatcc atgcattttt cggaagaaac
      721 tggctcatcc tattcgtcta cgtgattctg accactacgg ggccgttcgc cacatggaat
      781 acttttggct ttaccggcct tatattttac ctcgcaggcc tgatcctttt cagtattttg
      841 gtttctttgc aaagcaaaca gcctgtttgg cgcaaccttt tgctgatgcc ttttcagcag
      901 gtaatagtcg tgatgatcgc atttctggca gccttcaggc acagcaccgg caccttgaaa
      961 tggaagggga ggagggttta agacgaggaa aagggaaaaa ggaaaaggga aaaagtactt
     1021 ttgcagtatg attaaatact ttggaattga ctagtaaagc ttcagttatc ctggttccgg
     1081 gccgggaaag ttcggttctg cggcggcatc cctggatatt ttccggggct gtcaggaatg
     1141 ttcagggtaa tccgtcggcc ggtgagaccg tggaaatata ttcttcggca gggcagtggc
     1201 tcgccagggg aagttactcg cccgggtcgc agatcaggat ccgcatcctt acttttgacg
     1261 cgggagagac agtggatgaa acatttttcg aaaacaggat caggagggca gtggaacttc
     1321 gacaatctct gccgggattc cgggagggca attgtgcccg tctgattttc agcgaatcgg
     1381 atagtttgcc gggattgatc gtcgacaagt atgatgactt cctggtgtgc cagtttctat
     1441 ctgccggggc cgagttctgg aaagatatca taacccctaa acttcaggag attataccct
     1501 gccggggaat ctttgagcgc tccgattccg acagccgggc caaagagggg tttgcggagc
     1561 gaacaggttt gatttcaggg gaagaacctc ccgccagggt agcggttgat gacggtaaga
     1621 tgaagttttg ggtggatatc cggcaggggc ataagacggg attttatcta gaccagcgcc
     1681 ttaaccggct gaaaatcatg gattatgcca atggcagaga ggttttaaat gctttcagtt
     1741 atacaggggg ctttggcgtg gctgctgcta ttgccggcgc ttccacggta gttaatgtcg
     1801 attcgtcgaa agatgcactg gaactggcaa ccgagaattt ccggctgaac gggcttgcct
     1861 tggaaaagtc ggacttcgcc gaggacgatg ttttccagct attgcgcgaa taccgcgacc
     1921 ggcggcggca atttgatatg atcgtcctgg atccgcccaa gtttgtggcg tctgccagcc
     1981 agctggacaa gggcacgcgc ggttataagg atatcaacct gttggcattc aagctgctgc
     2041 ggccaggtgg aatactggtt acattttctt gctcagggta tgtaaaaccc gacctttttg
     2101 ctaagattgt ttccgatgca gcccttgacg ccggaaggga tgccgttatc gtggaaaatc
     2161 tccatcagtc ccccgatcat ccggtagcgc ttaattttcc tgaaggcctt tacctgaaag
     2221 gattgatctg ccgcgtaagt tgaaaattta acggtcagat tatcaggcta ttatttcatt
     2281 taaggccaga atgatccgga aggccagcac cgttagcagg catacgggaa atatccatga
     2341 tgcgaacgtc atttcactgg tttccttttt tgtcattaat aatacaggcg ggtcgagttt
     2401 atagcggatg aagatcatga cagcggggag attcatcatg accagcccgg caagttcttc
     2461 atacgggggc ccgggaagga aaaaaagagt ggcggcaatg gatgtgatga aaaggggaac
     2521 caaaatggag gccagcatga agtcgtgatt gccgagcctt gaatgattcc ggctcaataa
     2581 agctgcgtgt aagtaatagt ctttcagcaa cttgttagtt ataagcaaat agagtataaa
     2641 tgacagtatg ccggcgctga acagcaccag cggcccggca tacagccaat cgaatacaaa
     2701 aaagacattg gttcggacga ccaggttgaa aggggtcatc cccaggattc tataaaccag
     2761 aaagaatgca aaccaggtca ggaataaatc tttgtaatgg ggctgagggt ctttttcctt
     2821 tttcggaaga tatatcaata caattattat agccatgata accaacggca tcaggtagat
     2881 tatcaggaca ttctcctggg tccagttgga atcggcccct ttgattatta gtttgttgta
     2941 cagccaggtg attttgaaat caaacagaaa agctcccgtg ataaccagga gcttttctgc
     3001 gataaatgat aaaaagtaag cagttagtgt atacgaagaa atcctgaaaa ggaggttcct
     3061 gaaaattttc ccgttccccg gcaaatgatc ggaatgcata ggaaaattat tttttaagca
     3121 ggtccctgat ttctgtgagg agtttttctt cgttagatgg ttcgggtggc gccggttccg
     3181 gttccggttc agcgggtttg gccctgagtt gctgcatttt ggtcatcgac cggatcgcca
     3241 tgaagatcgc aaatgcaatg atcaggaaat cgatgatgtt ctggataaac atcccgtaat
     3301 tccaggtcac cgctgctact ccttcggctc cttcctttat aataagcttc aggtctttga
     3361 aatcaacccc tcccaggagc aatcccaggg gcggcataag gatgtcgttg acaaaagagg
     3421 tgacaatctt tccgaatgcg ccgccaatga tcacggctac agcaaggtcg atcacgttgc
     3481 ctttcatggc aaattccttg aattctttaa gcagtcccat attctttttg gtttaagtga
     3541 aacaataatt taaaagatat aaaaattcaa aatgactata aagatattta aatgttaaag
     3601 aatcataacc atttttttcg ccggaaataa atgagcattc cgacggttaa caaaaacatt
     3661 atgcccagaa tagtatagta cccgtatttc cagcccagtt caggcatatt cctgaaattc
     3721 attccgtata tccccgcgat aaagcttaac gggataaaga tcgaagtgat gatcgtaagc
     3781 aacttcatga cctggttcat ccgaagtgtc agattcgaca ggtagctttc ttttagttcg
     3841 gcgctgattt cccggtaagt atcgatatag tccagcacct ggaccatatg gtcgtaaata
     3901 tcgtcaaaat agggaagggt atctttcatc accaggtcgg attcgcctct cttcagtttg
     3961 ttaatggctt ccagtgaagg gatcagcgcc tttcgcaatt tgatgatctc ttttttgatc
     4021 tccctgtttt tatcgatcag ggtccggtcc ggattctcaa agatcagttc ttcaagctct
     4081 tccaggcggt ctcccaatga ttcggcaaca gcgtaatacc ggtcgataat gatatcgagg
     4141 agggaataaa ggataaaatc gtttccccgc tgccggattc tggtattgcc tgcttccatc
     4201 gctgccttga tggaatcgaa ccaggaatgg ctcctctcat gaaatgaaac gaggtatccg
     4261 ctgccgagga taaaactggc ctgctcatcc aggatctccc cttcgccgga aatgtaaaaa
     4321 gctttcaggg tcaggaaaag catgccgccc tgaatatcga atttggggcg gtgttcggta
     4381 tttaatatgt cttcaagaac cagagggctg atattgagtg ctttgccaag acggtccatc
     4441 agggttgtgt cgtgcaaccc gcatatctca aaccaattgt ttaccccctc cgatgttttc
     4501 agttcatgga gatcgattac ggcagtactg gaaaattcgg aggagttgaa agcccagtgc
     4561 ctcaccgtta cagaatcggc aatctttttg ccgatatgta cgagcgaacc gggcggtaat
     4621 ccggtctttc ctgacttcct ggtatctttt aacggaattt tattcctggg ggagtggttc
     4681 gttttttttc gggatttcat attcccgaaa ttacgattat tttagaatcc aattattcca
     4741 ttaataataa tcatcatctt tatcttcata ataggccagg tcatcgtact gatcttccga
     4801 aaaatcatcg ttcagatctt catcgaattc ttccaggtcg tctcccgggc cctccagcat
     4861 caaatcgttc ttgccgtcaa aatcttcaag atcaacctta tcgaaatcat cgccgtaagt
     4921 atctgcttct tcgtcggttt cataaatgaa ctcttcggcc aggaacaacg cctggtcata
     4981 gttcttccct ttcagttctt caaaaatttc ctgaaagtct tcatcgtcct tgaccatcgg
     5041 cttgatcctt tcgatatact tgttcaaccc tgaaatatca tcattttcaa gataattctt
     5101 gatgttttcc agtttctcga gcttcttctg atccattgtt actattattg taaaaagcgg
     5161 atgcaaatat atttttcctt tatttatctg caacattcaa aaataaattt ttattgtgat
     5221 tgtttaataa atgattgtgt ggtaaatatt cggttttatc cattttttca caccgataat
     5281 cgttaatttt gcccgttctt aagaaatgag ttttctttat ccatatatgt tatgggggtt
     5341 ggcagcggtt tccatccctg ttatcatcca cctgtttaat ttcaggcagt tccggaaggt
     5401 atactttacc aatgtccgtt tcctggatga gcttaagcaa cagacacgcc gccagtcgca
     5461 gttgcggcac ctgctggtga tgatcagccg gatgctggca attgcttcac tggtgctggc
     5521 ttttgcccgc ccgtatattc ctgttgaaaa ggagatgaca ccgcctcagg cggtgaacca
     5581 ggtgagcatt tacatcgata attccttcag catggaagct ttgtcgtcgg ccggcccgct
     5641 gctggaagtg gcccggaatg cggcgcgtga aattgcagcg ggttataaat cgaccgaccg
     5701 gtttatgctc acggtgaacg aatttgaagg ccgtcaccaa cgatgggtga accgtgacga
     5761 atttctgcag atgatcgggg agatcgtacc gacacccgct gtcaggatcc tctcggaagt
     5821 gatcaggcgg cagcaaaaca gtcttaccgg atcacacgga gaatccgtga ccagctacct
     5881 gatctccgac tttcaaacct cgatggcaga cctggagaat gttaaaccgg attcgctgaa
     5941 ccccttgttc ctggttcccc tgcaagccgt ccggtctgaa aacctgtata tcgacagttg
     6001 ctggttcgtc tcgccggtcc accagatcga ccagggagta aaactcgtgg caagagtggt
     6061 caacgattcc cggaccgatt acgagaaggt tccgctgaaa ctatccgtga acggcctcca
     6121 gaaggctgtg gccagctttg atattaccgc cggtaccaat tacgacgtcg aaatcccgtt
     6181 taccaaccat gaacccggaa tccagctggg aatagtcgaa ataaccgatt acccggttgt
     6241 atttgacgac cagatgttca ttgtgtacaa cgttgccggt tccatcccgg tgctggccat
     6301 caacggcaac caggagagcc ctttcctgaa ctctttgttc ggcaaagatc ccgaattcgg
     6361 ttttgtcaat aacccgctgg gaaatatcga ttacaaccgg cttcgtgaat ttgaactcat
     6421 tatcctgaac gaagttgaaa acctttctac cggattgatc caggaactga caatttacct
     6481 tgagaatgga ggctccttgc ttatcatccc gtctgaaaaa ctggatattg gatcctatac
     6541 tgactttcta cagtcagtta acgtgaattt ttacggtgaa tttacccgtg aactcacgcg
     6601 cgtgactgag atcgaccgcg ataaccctgt ttttgccgat gtttttgagc ggaatgtcgc
     6661 ttctgaaaga ggcgggaaca cgatggacct tcctgaggtc aggagctttt tcccggttac
     6721 ccgcaatgcc cgcacagcac aggttccggt tatgaccatg cagaatggca aaccattcct
     6781 gactatggaa aaagcgggaa aaggcctggt ttacctgctg gctgtcccac tggcggaatc
     6841 ctccggtaat tttgcccgcc atgccatctt tgtaccggca ttataccgga tcgcactcct
     6901 tagtgccgcc agcgatccgc tatactttgt gatcggaagg gataaccggg tatccatgtc
     6961 caatcccatg ctcagcggcg actatgtgct caggattgtt tcatccggcg gcgactatga
     7021 gttcatcccg ggaatgcaaa ctcagaataa tctttttaat attttgatca ataatcagat
     7081 acggtcggct ggccattatt tcgtcagaaa aggcgaagag gatatcaaag ggctgggatt
     7141 caactatgac cgaaatgagt cggttatgga attttccgga gcggaggaac tgaaccgaat
     7201 gattgacaaa tatggatttt cccggatgcg cgtcatttcg gaagatggga aacctttggc
     7261 ggaatcggtg aaagatatga accagggtac tgtcctgtgg aggttattta ttattttagc
     7321 cctggttttt ctggcttttg aaattgtttt attaagatac tggaaattaa aatcatgagg
     7381 attttacgcg aacagtctgc cgggctggtt atcgatattc aggagaaatt atttcctttt
     7441 atttcgggaa atgatgccct gaccagcagc tctgttatcc ttgtcaaagg attgcaggcg
     7501 ctgaaaatac ccataataac caccgttcag tataccaaag ggcttggccc tacgatccgg
     7561 cccctccggg agttgttgcc cgatgaagag acgattgaaa aactctcgtt cagttgctgc
     7621 gacgacccgg tttttatgaa gaaactggaa tcgatacaaa aaagattcat cattatcagc
     7681 ggcatagaat ctcatgtctg cgttttgcaa acggtgaccg acctgctgga aaaagggtac
     7741 gtgccggtag tggcggagga ctgtgtttct tcccgtaaac tcaatgataa aaatatggcg
     7801 attgcccgga tgcatcgcca gggggctatc attaccactt atgaatcggt gctgtttgag
     7861 ctgctccgtt attccggaac cgaagagttt aaagccattt ccaggctggt taaataatat
     7921 ttgttaatac agggatggat gagatcaggg aagatatgac cgaaggacgg gaagagccgg
     7981 gaaacctcaa ttccgaacaa tccccaggcg ccgataccca tcggacgatc aagatatcag
     8041 ggctttacga gaactggttt cttgactatg catcctatgt cattttagag agggcggtgc
     8101 cggaagtgat cgacgggctg aaacctgtac agcggcgcat tctccattcc atgaagcaac
     8161 tggatgacgg gcggttcaat aaagtggcca acatcatcgg ccataccatg caataccacc
     8221 cgcacggcga tgcttccatc ggggatgccc tggtccagct ggggcagaaa gacctgctga
     8281 tcgaaacgca ggggaactgg ggcaacatcc ttactggcga cagtgcggca gcgccacgct
     8341 acatcgaagc caggctttca aaatttgccc tggaggtcgt ctttaatcca aagactactc
     8401 cctggaaacc atcttacgac ggccggaaca aagagccggt caccctgccg gtcaaattcc
     8461 cgctgctgct ggcccaggga gtggaaggaa tcgccgtcgg acttgcatca aagatccttc
     8521 cccacaattt tattgagctg atcgacgctt ccatcaacat cttaaaagaa gaagaattcg
     8581 aaatctatcc cgattttcag accggcggac tgatcgatgt ttcgaggtac aacgacggcg
     8641 cccggggagg caaggtgagg atcagggcga agatcaaaaa gatcgacaac aaaacgctgg
     8701 tcatacagga aattcccttc ggcgttacaa ccaccaacct gatcgattcc atcatccagg
     8761 ccaacgacaa gaagaagatc aagatcagga aaatcgacga taatacggct gccgaagctg
     8821 aaatcctcgt ccatcttgtt cccggtacct cacccgatca gaccatcgac gccctttacg
     8881 ctttcaccct ctgtgaggtg cctgtctcgc ccaatgcgtg cgtgatccgc gacggaaaac
     8941 cgcaatttct ggatgtcagg gagatcctcc gtatttccac ccggaatacc gtcggactga
     9001 tccggcgcga actggagatc aggctggatg aattgcgtga acaatggcat tatgccagcc
     9061 tggagaagat ctttatcgaa cataaaatct accggaagat cgaaacctgt gaaacctggg
     9121 aagaggtgat cgaaaccatc gacaaagggc tggcgccgtt tacgaaaaag ctggtaaggc
     9181 ccgtaacgca tgaagatatt gtcaggctga cagaaatcag gatcaaaagg atctcaaaat
     9241 ttgattccaa caaggccgac gattctatcc tttcgctgga gaatgagatg gaagaggttc
     9301 aaaaccacct cgatcatctt atcgattatg ccattctcta tttcgaacag atcaggaaaa
     9361 agtacagcgc cggccgtgag cgtaaaacag agatcaggag tttcgatacg atcgaagcgg
     9421 cggccgtggc agcagccagc cagaaactct atttcaaccg tgaagaggga tttgccggca
     9481 catccctgaa aaaagacgaa tatatctgcg attgctccga tatcgacgat gtgatcctct
     9541 tccgggaaga cggaaccttc ctggtcagga aagtggaagg gaagtttttt acgggaaaag
     9601 atgtcgttta cataaatgtc tttaaaaaga atgatgaacg gacgatatac aatatggtct
     9661 accgggatgg caaaaagggg tcgtgttatg tgaaacggtt cgccgtggtt ggcgtcacgc
     9721 gggataaaga atacgatctg acaaagggaa accccggatc aaaggttgtt tactttagtg
     9781 ccaatccgaa cggggaagcc gagatgatca ggattttcct gcgcccgaaa cccaggatga
     9841 aaaaaaccac tttcgatttt gacttcagtt ccattgccat aaaaggcaga agttcgcagg
     9901 gaaacctgtt atccaaaaac ccggtacgcc agatagttaa aaaagaacat ggggtatcga
     9961 cgctcggcgc ccggggcata tggtatgacg aatcggtcaa acggctcaat gctgaagaga
    10021 ggggaatctt tctcggtgct tttgaaggcg
//
LOCUS       DAOPNH010000107        10011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7308_length_10011_cov_6.710125, whole genome shotgun sequence.
ACCESSION   DAOPNH010000107 DAOPNH010000000
VERSION     DAOPNH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_10011_cov_6.710125"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1505)
                     /locus_tag="PKZ74_07075"
     CDS             complement(36..1505)
                     /locus_tag="PKZ74_07075"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HPM92559.1"
                     /translation="MKKQFLSSFFKLTLLVLMGTLIFVGCKDDDDDDPPIVVLDGFYI
                     KGAATALPDLADNGRMSVARNEVNQTDRADLFEMYIPIKAGTDGFNIIMVKGDTLKTY
                     GPGADFALVPEAELDVEEPKEGLWKGSLVETETKFTVPEDALYHVMFDRELMKIAIAK
                     VVWGVIGGATPGGWSESTPMTAGTFGTSEMSFEVAEILLLVNDYKFRYSNGWKIILDA
                     DYDLGGGSKGIKLNTNFGGAVEALVPGGGNIANDTYGVWKFTMTWKLGEGTKATKTWV
                     KDGEPLPEYPETLFMIGATVGGWDWATIDLPMIPVHSHPELFWKIVWMEAGVADAGFK
                     FAPNKEWVGDFGKTGDATNGVYAKGGENVPGPETSGFYMVVVDLENETIEVNAPTVYM
                     IGDAVGSWDAGVAANLFTVDNANSVVTITKDLAAGNLRMYAAATTLAADWWQAEFMIF
                     NNIIEYRATGGDQAAVPVTAGNYTINLNFKDNSGSVTQN"
     gene            complement(1627..3129)
                     /locus_tag="PKZ74_07080"
     CDS             complement(1627..3129)
                     /locus_tag="PKZ74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM92560.1"
                     /translation="MKKISLLYLALFITLFSCTKLDEEVYDKIPGDLYPENENQIANL
                     SVEAYAKLKPLCDDEGWWFLAQEISSDELCGPTRGSDWFDGGKWVNVHRHTWSNDEES
                     VNRMWEKFWTGITTCNQVLDMMKELPQNDALVAKMREVEVLRAFYYYLLIDNYGDAPY
                     LSSAANAPEKPYKAKREDIFDSLVTTVKNSLPYLKAIDNKYMANRYMAYALLTKLYLN
                     AEVYTGTPMWEEALIYCDSILAGPYAMEETNFMAPFLTNNEKNKEIIFSIPYDEDNFQ
                     GFRLHMRTLHYQHNLKYDMPVGPWNGFCVVPTFFDTYDDSDLRKSKYHIYGMQYDTKG
                     DAIIDGETGQPLDISPYVPALYMQAADYTPQQIRTTGARIGKYEIKTGAKENLSNDFP
                     LFRITDFNLIKAELEIRLGRSGDEWIMPVRERAGVAELPPTSGWTLDQLLAERGRELY
                     VEGHRRQDLIRYGKWEDAWWEKEAHGTDRRTFPIPKWATDANPNLLENPR"
     gene            complement(3143..6154)
                     /locus_tag="PKZ74_07085"
     CDS             complement(3143..6154)
                     /locus_tag="PKZ74_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM92561.1"
                     /translation="MKHLLIIVFFLGTTASLLAQNMQVSGVITDESTGETLIGATVLE
                     KGTTNGTVSDLDGKYTISVPKGATLAFSLVGYEPEEFVIEKSGTLNVAMKVQVTNLDD
                     VIVIGYSTQKKSDKTGAVSLVKADEMNSGVITSPIQSMQGKAAGVLVTKKGGDPNESF
                     SVRIRGASGFNSNTQPLYVIDGIPGADPTILSPSDIESFNILKDAASTAIYGSRGSNG
                     VIIITTKKGKKSGIPAEGDKTEGAFSNIEFTSQVSFEQISKKLGILDADGMRSFANKL
                     LEEAQVENPNYTIDSVFTDGGASTDWQDEIYRWGVSTVNNLSFSGGNKHNLYLASITN
                     SGWQGIMEGTEKKLTTARVNVTHKGFNDRLTITGNLMGAFEKNDYENYDGWDKDDIIY
                     QAISRNPTDPVYDSTGEYYKTSRVFNYENPLAIINYVTNKRDANKFLGGVKADFEIVK
                     GLVAGVNLGYINDNQTSTYFRPSGIYSSADNGYGKKEYKNNTQKLIEITGTYNKTFNK
                     NHNFNALLGYSWQESVYSGFYSQGGDAQSNYAGPDNLAVLNDVKWGDIGSWKGKWNLI
                     GFFGRVQYNYKSKYYASASLRRDGSSKFGSNNKWGWFPTAAIGWNLDQEKWLSDVDWL
                     SQLKIRASYGVSGNQEIGEYRSMVLWEPSGKAINPETGQEVITFKPAWNANPDLKWEE
                     TSEANLGIDFAFLNSRLSGSLELYYKITNDLLGEYNVPVPPNLATKTFANSGSMSNRG
                     IELYLQAFIISNTNFSYKSSLTASHNKTEILDLGDYFNEEDGVRKEGYISGRGMVGEE
                     YYVTGIIVGEETGSFYVPTFITLKDGKFIYESKTGGFTDVLSDAKRTVVATAAPKVEL
                     GWSNTLTFRKRWNLDFALRSWIGNHVYNATEMFFDNPGNLPSLNALPSAIEWYDQDRV
                     TGSTIADIYVENASFLKLDYISLSYEFDVSKVNWLQGLSLYVSANNVLTITGYSGVDP
                     ETTVNGLSFGIDQYNVYPKTRAYTAGLKVNF"
     gene            6456..6854
                     /locus_tag="PKZ74_07090"
     CDS             6456..6854
                     /locus_tag="PKZ74_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92562.1"
                     /translation="MKRSIVIFIIAAMVLITTGFWILSPGAIGDTMEYLHFGIILLIV
                     LFALFIGYKRLKSEKRGEPAEDELSKKVLQKTAAWSYYISLYIWVAMIYIKDRIVMDT
                     EIMLGTGILAMAVTFAVCWLVFNFRGIRNE"
     gene            6847..7056
                     /locus_tag="PKZ74_07095"
     CDS             6847..7056
                     /locus_tag="PKZ74_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM92563.1"
                     /translation="MNNRIREFRARYNLTQDDLALKVGVRRETIVFLEKGKYNPSLKL
                     AHDISVVFNVKIEEIFLFDDPAPST"
     gene            complement(7104..7556)
                     /locus_tag="PKZ74_07100"
     CDS             complement(7104..7556)
                     /locus_tag="PKZ74_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92564.1"
                     /translation="MDRLKLKQKILDACFEQQTKVVDNLKVVMEEVADSAEEHGLPKD
                     LYDSYRNQMMSKKEMFAQQLLKVNEQIDILRRVDMTRVFQQVRFGAVVVTESQRLFIA
                     SGIGKIIVEGLEYFVISAMVPFYSAIDGKKPGDSYEFRGKKEKILEVF"
     gene            7672..8223
                     /locus_tag="PKZ74_07105"
     CDS             7672..8223
                     /locus_tag="PKZ74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPM92565.1"
                     /translation="MEKINPPYTEPENSLSVLELLRVAHEYCIYCEKASEKPFSENVD
                     FILKISPLLYLKGKLLPEINPEYPEFAERYVTEEEWQEVFNHFRSVYLEKDEFYNVSY
                     EDHHESEAVKSSLSENLSDIYQDLKDFILLYQKNTQAARQNAVSQCRDLFETRWGPKL
                     LSNLRYLHFLSVKERLPGMDDER"
     gene            8347..>10011
                     /gene="feoB"
                     /locus_tag="PKZ74_07110"
     CDS             8347..>10011
                     /gene="feoB"
                     /locus_tag="PKZ74_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPM92566.1"
                     /translation="MRSLRELKEGEKGIIIRIRGKGEFRKRIIEMGFVKGKRVTVIKA
                     APLQDPVEYRILDSDVSLRLEEADLIDVIPEAESPGFLEPGNFSSIFNEPDPLEEFHR
                     NGKIIHVALVGNPNSGKTSLFNHASHSREHVGNYAGVTVDSKTAEVKTGDHIIRLTDL
                     PGTYSLSSYSPEELFVRNHLSEKRPDLVINVIDASNLERNLYLTTQLVEMGLNVIVAL
                     NMYDELQIKGDRFDYEMLGKMTGIHFVPTIASKGKGIKELFAKIIDVYTGKTVTKRRI
                     RLDYGPEVELSVSRLMSRFHGTIVGESFPDRYFAVKLLERDWHALHYLEHKCKNPDIC
                     KLAEAETQRLEKIFNEDTESLIAGIRYGFIEGALKETYIPSTTPRMTLTRRIDFFLTG
                     RFAGYPIFILFMWLMFQATFIFGDYPVYLIEKLVEFTGNLLDRIIPDGIINDLVVDGI
                     IGGVGGVIVFLPNILILFLFISLMEDTGYMARVAFIVDKLMHKIGLHGKSFVPLLMGF
                     GCNVPAIMATRTIESRSDRMVTMLIIPFMSCSARYPVYILLISAFFV"
BASE COUNT         2562 a         2391 c         2265 g         2793 t
ORIGIN      
        1 ggcagcctct tctttttgat atagataaat cgcggttaat tctgcgttac tgaacccgaa
       61 ttatccttga agttcaggtt gatagtgtag tttcctgcgg ttaccggaac ggcagcctga
      121 tcgccaccgg ttgcgcggta ttcaatgata ttgttgaaaa tcatgaattc agcctgccac
      181 caatcagcag ccagtgttgt agctgcggca tacatgcgga ggttaccggc ggctaaatcc
      241 ttggtgatcg taacaaccga attggcatta tcgactgtga agagattggc agcaacgcct
      301 gcatcccatg agccgactgc atcaccgatc atataaacgg tcggggcatt cacttcgatg
      361 gtctcatttt ccaggtcaac cacaaccatg tagaatcctg atgtttcagg tccggggacg
      421 ttttcaccgc ctttggcata aacgccgttg gtggcatcac cggtctttcc gaaatcacca
      481 acccattcct tgttgggagc aaatttgaat cctgcgtcgg ctacaccggc ttccatccag
      541 acgatcttcc agaagagttc cggatggctg tgaaccggga tcatcggcag gtcaatcgtt
      601 gcccagtccc agccgccaac ggtggcgccg atcatgaaca atgtttcggg gtattccgga
      661 agcggttcgc cgtcctttac ccatgtctta gtggctttgg tgccttcgcc cagtttccag
      721 gtcatggtga acttccatac gccgtatgta tcattggcga tattgcctcc accgggtacg
      781 agggcctcga ctgcacctcc gaagttggtg ttgagcttaa tccctttgct tccgccgccg
      841 aggtcataat cggcgtcgag gatgattttc cagccattgg agtagcggaa cttgtaatca
      901 ttcaccagga gaaggatttc agcgacttca aatgacattt cgcttgtgcc gaaagttccg
      961 gcggtcattg gagtgctttc gctccagcca cccggggtag ctccaccgat cacgccccaa
     1021 accacctttg caatagcaat cttcatgagt tccctgtcga acatgacatg atacagagca
     1081 tcctcaggaa cggtgaattt ggtttcagtt tcaacgagcg aacctttcca taagccttct
     1141 ttcggctctt ccacgtcgag ttcggcttcc ggcaccaggg cgaagtcggc tccgggtccg
     1201 taggttttca gggtgtcgcc cttcaccatg atgatgttga atccgtcggt accggctttg
     1261 atcggaatat acatttcgaa aaggtcggcg cggtcggtct ggttaacttc attgcgggca
     1321 acggacatac ggccgttgtc agccaggtcg ggaagcgcgg ttgcggctcc cttaatgtaa
     1381 aaaccgtcaa ggactacgat tggcggatca tcgtcatcat catccttaca tccaacgaaa
     1441 atcaatgttc ccatcagaac aagcagggtc agtttaaaga agctgcttaa aaattgtttt
     1501 ttcatactta atttagttag attagttaaa aaattgaatt gcaataacat taatttcaaa
     1561 ctctttggct ttcaaatgcg aaattacaaa taaattgatt ttatttcaat atttaattca
     1621 ttcaatttac cggggatttt ccagtaaatt cggattggcg tccgttgccc atttcgggat
     1681 cgggaatgtc ctccggtcgg tcccgtgagc ttctttttcc caccaggcat cctcccattt
     1741 tccgtaacgg atgagatcct ggcgcctgtg cccttccaca taaagctctc ttcctctctc
     1801 agccagcagt tgatccagtg tccaaccgct ggttggcgga agctcagcga caccggcacg
     1861 ctccctgacc ggcatgatcc attcatctcc gcttctaccg agcctgatct ccagttcagc
     1921 tttaatcagg ttaaaatcgg tgatcctgaa taacgggaaa tcgttgctta aattttcctt
     1981 ggcccctgtt tttatctcgt attttccgat cctggcgcct gtcgtcctga tctgctgcgg
     2041 agtataatcg gctgcctgca tataaagcgc cggaacataa ggactgatat ccaggggctg
     2101 tcccgtttct ccgtcgataa tcgcatcacc cttggtatca tattgcattc cgtaaatatg
     2161 gtatttggat ttccgaaggt ccgaatcatc atatgtgtca aagaacgtcg gaacgacgca
     2221 aaaaccgttc catgggccga ctggcatatc atatttcagg ttgtgctgat aatggagcgt
     2281 acgcatatgc agccggaaac cctggaaatt atcttcatcg taaggaattg agaaaatgat
     2341 ctccttgttc ttttcgttat ttgtcaggaa cggtgccata aaattggtct cttccatggc
     2401 ataaggaccc gccaggatgc tgtcgcaata aatcaaggct tcttcccaca taggcgtacc
     2461 cgtataaact tcagcattca ggtaaagctt tgtgagcagt gcatacgcca tatagcggtt
     2521 ggccatgtat ttgttatcga tggctttcaa atagggtaat gagtttttta ccgtagtaac
     2581 aagactgtca aagatatctt cccgttttgc cttgtaaggt ttttccggtg cattggccgc
     2641 gctcgacagg tacggggcat ctccataatt atcaatcagc agataataat agaacgctct
     2701 caacacctcc acttcgcgca ttttggcgac gagggcatca ttctggggaa gctctttcat
     2761 catatccagg acctggttgc aggttgtaat gcctgtccag aatttttccc acattcggtt
     2821 cacgctctcc tcatcgttcg accatgtatg ccggtgaaca ttcacccatt tacctccgtc
     2881 aaaccaatcg cttccccgtg tcgggccaca aagttcgtcg gatgaaattt cctgggccaa
     2941 aaaccaccat ccttcatcat cgcacaacgg tttaagtttg gcatacgcct caacgctcag
     3001 gttggcaatc tggttctcat tttccggata tagatcgcca gggattttat catacacctc
     3061 ttcatccagt ttggtgcagg agaaaagggt aatgaacaat gctaaatata ataaactgat
     3121 ctttttcata gcttttctgg gattagaagt taaccttcag gccggccgta taagcgcggg
     3181 ttttgggata aacattgtat tgatcgatgc cgaatgacag gccgttgaca gtggtttcag
     3241 gatccacacc ggaatagccg gtgatagtaa gtacgttgtt ggcagacaca tagagtgaaa
     3301 gtccctgcag ccagttcacc ttcgacacat caaactcgta cgacagtgaa atatagtcca
     3361 gcttaaggaa tgaagcgttc tcaacgtaga tgtctgcgat cgtcgatccg gtaacccgat
     3421 cctggtcata ccattcaatt gccgagggca gtgcattgag tgaaggtaaa ttgcccgggt
     3481 tgtcgaaaaa catttccgtt gcattgtaaa catggtttcc gatccaggaa cgtaaggcaa
     3541 aatcgagatt ccagcgtttg cggaaggtga gggtattcga ccatccaagt tcaacttttg
     3601 gcgctgcagt ggctactacg gtccgtttgg catcgctcag cacgtcagta aatcctcctg
     3661 ttttggattc atagataaat ttcccatcct tgagtgttat aaaagtcgga acatagaaag
     3721 aaccggtctc ttctccaaca ataatcccgg taacataata ttcttcaccg accatccctc
     3781 ttcctgaaat atatccctct tttctgacgc cgtcttcttc attaaaataa tccccaagat
     3841 caagaatttc ggttttatta tgagaagccg tcagactcga tttatagcta aaattggtat
     3901 ttgaaatgat aaatgcctgc aggtaaagct ctatcccccg gttagacatc gacccggaat
     3961 tggcaaaagt tttggtcgcc aggtttggtg gaaccggaac attatactct ccaagcaagt
     4021 cattggtgat cttgtaataa agctcaagac taccactaag ccggctgttc aggaaagcaa
     4081 aatcgattcc gagatttgct tctgaagtct cttcccattt aaggtcggga ttggcattcc
     4141 atgcaggctt aaaagtgatg acttcctgcc cggtctccgg attgatggct tttccgctgg
     4201 gttcccataa aaccatactg cggtactcac cgatctcctg gttgccggaa acaccgtaac
     4261 tggccctgat cttcaactgg ctcagccaat caacatccga tagccatttt tcctggtcga
     4321 ggttccagcc gatcgctgcg gtcgggaacc atccccattt attgttagaa ccgaatttgg
     4381 atgaaccgtc gcggcgtagt gaagccgaag cataatattt gcttttataa ttgtattgaa
     4441 ccctgccgaa gaaaccgatc aggttccact ttcctttcca tgagccaatg tcgccccatt
     4501 tcacatcgtt gagtacggcc agattatccg gcccggcata gttcgactgg gcgtcgccac
     4561 cctgtgaata aaaaccgctg taaaccgatt cctgccagga atatcccagt aaagcattga
     4621 aattgtggtt tttgttaaag gttttattgt aggtgccggt tatttctatc agtttctgcg
     4681 tgttgttttt gtactccttt ttgccataac cattgtcggc agaggaatat attccggaag
     4741 gacggaaata tgttgaagtc tgattatcat taatataacc tagattgact cctgcaacca
     4801 atcccttcac aatttcgaag tcggctttta ccccacccag gaatttgttg gcgtcgcgct
     4861 tgttggtgac gtaattgatg atcgccagcg gattttcata attgaaaaca cgggaagttt
     4921 tataatactc tccggtcgaa tcgtataccg gatcggtagg attccgggaa atagcctggt
     4981 agataatatc atctttatcc catccgtcat aattttcgta atcgttcttt tcaaacgcac
     5041 ccatcaggtt accggtaatg gtgagccggt cattgaaccc tttatgcgtc acgttgacac
     5101 gggcagtggt cagttttttc tcggttcctt ccataatccc ctgccatccc gaattggtga
     5161 tggaggcaag gtagaggtta tgtttgtttc caccggaaaa actaaggttg ttgaccgtag
     5221 atacgcccca cctgtatatt tcatcctgcc agtcggtcga ggcgcctcca tctgtaaaaa
     5281 cgctgtcaat agtgtaattt ggattctcaa cctgtgcctc ttccaggagt ttgttggcaa
     5341 aagatctcat cccgtccgca tcaagtatgc ccagcttctt ggagatctgc tcgaaagata
     5401 cctgggaggt gaattctata ttgctgaaag ccccttctgt cttgtcacct tcagccggga
     5461 taccgctttt cttccctttc tttgtggtaa tgatgatgac accgttcgat ccccttgacc
     5521 cgtagatagc ggtcgaagct gcatccttca aaatattgaa agattcgata tcactgggtg
     5581 aaagaatggt cggatcggcg ccgggaatcc cgtcaatgac atataaaggc tgggtattgg
     5641 aattaaaacc ggaagcgcca cgtatcctga cggagaatga ctcattcggg tcgccccctt
     5701 tttttgttac cagcacaccg gccgctttac cctgcatcga ctggatcggt gaggtgatta
     5761 ccccgctgtt catctcatcc gctttcacca ggctgacagc accggtctta tccgatttct
     5821 tctgggtgct gtaaccgatg acgatgacgt cgtcgagatt tgtcacctgg actttcatgg
     5881 cgacattcag ggtccccgat ttttcgataa cgaactcctc aggctcatac ccgacaaggg
     5941 agaaggccag tgtggcccct ttaggtaccg aaatggtgta tttgccatcc aggtcgctga
     6001 cagtaccgtt tgtggttccc ttttcaagca ctgtagcgcc aataagcgtc tcacccgtgc
     6061 tttcgtcggt gatcacaccg gaaacctgca tgttctgggc caacaggctg gccgtcgtcc
     6121 ccagaaaaaa aacgatgatt aacaggtgtt tcataattat tctaagttta tttttcatat
     6181 ttatgaagat tagtttctgg aaatcgggtt aagaataaga tagtcgaaca ctgcaaaata
     6241 gtgatttcgg ggcctgagtt ttgaacctcc caaaataatg aaccatgtaa aactttgcaa
     6301 aatgctgaat caacggcaca ttccatgcat ccttggttat aaagcacagc aaaaataata
     6361 aaataaatgt taaaaaaaga ttaaaataat tttggattag aatgttattt atttctaact
     6421 ttgtgttagg tatttataac atcaaattta ttcatatgaa aagatcaatt gtaatattta
     6481 tcattgccgc aatggtgctg atcacaaccg gtttttggat tctcagcccg ggggcgattg
     6541 gagatacaat ggagtatctg cattttggta tcatcctgct gattgttctg ttcgccttat
     6601 ttataggcta taaacgcctg aagagcgaaa aaagaggcga gccggctgaa gatgaactgt
     6661 caaaaaaagt actgcagaaa actgctgcct ggtcctatta catttcgctc tatatctggg
     6721 ttgccatgat ctacatcaaa gaccgtattg tcatggatac cgaaattatg ctgggaacag
     6781 ggatcctggc aatggcggtc acttttgccg tttgctggct ggttttcaac ttccggggga
     6841 tcaggaatga ataaccggat cagggaattc agggccaggt acaacctgac acaggacgac
     6901 ctggcgttaa aagtcggcgt aagaagggaa accattgttt ttttagagaa aggcaagtac
     6961 aatccctcgc tgaaactggc tcacgatatc tcggtggtgt ttaatgtaaa gattgaagag
     7021 atctttcttt ttgatgaccc cgcaccctca acctgactaa cgactgacca ccaacgactt
     7081 atgactacac atatcgacct gcactaaaac acctccagga tcttctcctt ctttcctctg
     7141 aattcgtagg aatcgcctgg ttttttgccg tcgattgcac tgtaaaatgg gaccatggcg
     7201 gaaataacga aatattccag gccttcgacg ataattttgc cgatgcctga cgctataaat
     7261 agtcgctggg attcggttac gacgaccgcg ccaaacctga cctgctgaaa aacacgggtc
     7321 atatccaccc gccgcaatat atctatttgc tcatttacct tcaacaattg ctgggcaaac
     7381 atctcttttt tactcatcat ctggttgcgg tacgaatcat acaaatcttt cggcaggcca
     7441 tgctcctctg cgctgtcggc cacttcctcc atcaccactt tcaggttatc caccaccttg
     7501 gtctgctgtt caaaacaggc atccaggatc ttttgcttca gtttcaaacg atccatacta
     7561 tatttataat atttttaaaa attaaaaatc ttatcgcgaa aactcacgag aagattttga
     7621 aaggtaaaag caaataacaa attttgtacg aaaataataa tattttcatt tatggaaaaa
     7681 attaatccgc cttacaccga accggaaaat tctcttagcg tacttgaatt gctccgcgtt
     7741 gcccatgaat attgcattta ttgtgaaaaa gcctcagaaa aaccattttc tgagaatgtg
     7801 gattttatat tgaaaatcag cccgttgctg tatctaaaag gcaaactgct tccggaaatc
     7861 aacccggaat acccggaatt cgccgaacgg tatgtaacag aagaggaatg gcaggaagtg
     7921 tttaaccatt tcagatcggt gtaccttgag aaggatgagt tctacaatgt cagttacgaa
     7981 gatcaccatg aatcggaagc cgttaaatca agcctttccg aaaatctttc ggatatatac
     8041 caggacctca aagatttcat cctcctgtac cagaaaaata cacaggccgc ccgccaaaat
     8101 gccgtcagtc agtgcaggga tctgtttgaa acacgctggg ggccgaagtt gctgagcaat
     8161 ctgcgctacc tgcatttcct ctccgtaaag gaaaggttgc cgggtatgga tgatgagcgt
     8221 tagtcgtaag tcgttagtcg caaaaaactc tgccacttat ttagaaataa tttagaaccg
     8281 ctaccctgct gacctacttc ttatttcgat atttttgtcc ccgttatctg gaattaatct
     8341 aaataaatga gaagcctcag ggaattaaaa gaaggagaaa aagggatcat tatccggatc
     8401 aggggaaaag gtgagttccg taaaaggatc atcgaaatgg gctttgtgaa aggaaaacgg
     8461 gtgaccgtta ttaaagccgc cccgttacag gatcctgtgg aataccggat acttgattca
     8521 gatgtatctc tgaggcttga agaagcggat ctgatcgatg tcatcccgga agcggaatcg
     8581 cccggattcc tggaaccggg gaatttcagc agcatcttta atgagcctga tccgctcgag
     8641 gaattccata gaaacggaaa gatcattcat gtagccctgg ttggaaaccc taattcgggc
     8701 aaaaccagcc tgttcaacca tgcttctcac tcccgcgaac acgtgggaaa ttatgccggg
     8761 gtaacggtgg attcgaaaac agcggaggtg aaaaccggcg accatatcat ccgccttacc
     8821 gacctgccgg gaacctactc gctctcatcc tacagcccgg aagagctttt cgtcaggaac
     8881 catctgtcgg agaaacgtcc cgacctggtc atcaacgtga tcgatgcttc caacctggag
     8941 agaaatcttt acctgaccac acagttggta gaaatggggc tcaatgtgat tgttgccctg
     9001 aatatgtatg atgagcttca gatcaaaggc gaccggtttg attacgagat gctgggcaag
     9061 atgacaggta ttcatttcgt ccctaccatc gcgtctaaag gaaagggaat caaagaatta
     9121 tttgccaaaa taatcgatgt ttataccggt aaaaccgtta caaaaagaag aatccgcctg
     9181 gattacgggc cggaggtgga attgtcggtc agccgcctga tgagccgttt ccatggtact
     9241 attgtgggcg aatccttccc cgacaggtat tttgcggtca aattactgga aagggactgg
     9301 cacgctttgc attatttaga gcataaatgc aaaaatcccg atatatgcaa actagctgag
     9361 gctgagaccc agcggctgga aaagatattc aatgaagaca ccgaaagcct gattgccggt
     9421 atccggtatg gatttatcga gggagctctg aaagagactt atatcccgtc gacaacgccc
     9481 aggatgaccc tgacccggcg catcgatttc tttctgaccg gccggtttgc gggatacccg
     9541 atttttatcc tcttcatgtg gctgatgttc caggccactt ttattttcgg tgattatccg
     9601 gtttatttga ttgaaaaact ggttgaattc accggtaatt tattagacag gatcataccc
     9661 gatggcatca tcaatgatct ggtcgtagat ggcattatcg ggggtgtagg tggcgtgatc
     9721 gttttcctgc cgaatattct catcctgttt cttttcattt ccctgatgga agatacgggg
     9781 tatatggccc gtgtggcttt tatcgttgac aagctcatgc ataaaatcgg gcttcatggc
     9841 aaatcgttcg tcccactgct gatgggcttc ggctgcaatg tcccggcgat tatggcgacc
     9901 cgcaccattg agagccgaag cgaccggatg gttacgatgc tcatcatccc tttcatgtcg
     9961 tgctcggccc gctacccggt ttatatcctg ctgatcagtg cgttctttgt c
//
LOCUS       DAOPNH010000108         9982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7342_length_9982_cov_7.595749, whole genome shotgun sequence.
ACCESSION   DAOPNH010000108 DAOPNH010000000
VERSION     DAOPNH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_9982_cov_7.595749"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKZ74_07115"
     CDS             <1..787
                     /locus_tag="PKZ74_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPM92567.1"
                     /translation="VIREEIAPLSAREIKGLKWAGVTVFTFMVLMAWELIPENGFLRG
                     QDGTVLHSPVLKGFISVLFIMAAVSGIVFGYVSGTFKKHDDVIAGMNSSFKSLVSFLV
                     LVFFAAQFVAWFKWSNLGLLIAVKGADILQSADIGLIPLVILFVILSGFINLFMGSAS
                     AKWAIMGPVFIPMFMLLGYSPELSQAVFRIGDSVTNVISPMMSFFALIIVYYQKYEPK
                     AGLGTLIATMLPYSVTFFLAWTALLIIWILFGLPLGPEAGLYY"
     gene            874..1377
                     /locus_tag="PKZ74_07120"
     CDS             874..1377
                     /locus_tag="PKZ74_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPM92568.1"
                     /translation="MKEKSIELLNKAVADEMAAVHQYMYFHFRCDDLGYFLISNLFKR
                     TAIEEMMHVETLAERILFLKGEVEMKAAHDVQKIHDVKQMLELATKMETDSAIDYNNW
                     AMECAKNADSATKKIFEALVDDEERHFDQYDKELDNLAKFGDNYLALQSIENSKNIAA
                     AGGRPAE"
     gene            1627..1935
                     /locus_tag="PKZ74_07125"
     CDS             1627..1935
                     /locus_tag="PKZ74_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92569.1"
                     /translation="MKNAIIIGALVILGATGLILGLSGYHVGEAGDFALGYFSAGQFS
                     AGVFSAGMFSAGIFSVGLFSVGIFSLSVFNVAVYGLGFFLIAWKKHYAKVKVVEEEKI
                     "
     gene            1991..3214
                     /locus_tag="PKZ74_07130"
     CDS             1991..3214
                     /locus_tag="PKZ74_07130"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HPM92570.1"
                     /translation="MMTYTESYHTLKSIFSTSQKDKTYNGTEIHEFPFISSSCGILPG
                     NPDHFCEKHHQHLSTSFSFYNDVGTIRDDVHIKDIEVKENQAFNYNIIRPAGEGKCRK
                     VIFLFHGFNEKDWTKYLPWAYALCSGTQSAVVLFPIAFHMQRAPKYWSSKREMFRLSE
                     ERRKRFPHIIHSTLSNVAISMRLHSMPQRFIWSGLQTYYDMIRFIEECRQGRHPDIDL
                     NFEFNIFAYSIGGFLAQILKLTNFSGYFDDTKVCLFCGGAAFNRLSPVSKFILDSEAN
                     VALYSFLVEHFDKMLDQDHLLHHYIRDDHPEGRVFYAMLDYRRSRSFREELLKKHEKW
                     FYAISLRKDEVVPSFEIINTLQGAYRDIRIRVDEVDFDRKYLHENPFPVNVAYSGQVD
                     EDFALVFDKACRFLA"
     gene            3376..3834
                     /locus_tag="PKZ74_07135"
     CDS             3376..3834
                     /locus_tag="PKZ74_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPM92571.1"
                     /translation="MAFYQLTKKQFVPASIDEVWDFISSPKNLKEITPEYMGFDITSE
                     NLPEKMYAGMIISYKVRPLMRIPMTWVTEISQVVDKQYFVDEQRSGPYAFWHHQHLIE
                     PYENGVMMTDIVSYKPPLGFLGSIANALMIRKQLEGIFAYREKALKERFP"
     gene            3958..4845
                     /locus_tag="PKZ74_07140"
     CDS             3958..4845
                     /locus_tag="PKZ74_07140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM92572.1"
                     /translation="MMNGDIDSGKRSFELRSLGATGFDTVYKAFSLAFQDYEIRLNRS
                     QLMAMLHRRGFDPGLSFAAFDGDDITAFTLNGIGTFNGIPTAYDTGTGTLKEFRGQGL
                     ATKIFEYSIPFLKEKGVEQYLLEVLQHNTGAVSVYKNLGFEVTREFNYFIKNMNEIRD
                     EILTQDIPFSIRQTGLDELKAASEFWDFHPSWQNGFESINRSAGEFVCLGAFHEHRLA
                     GYCIFEPLTGDVTQIAVSRPYRRKGIGSFLLREMIGRNRSEVLKIINTEISCESITRF
                     LASRNIEPTGRQFEMIRKL"
     gene            4940..5506
                     /locus_tag="PKZ74_07145"
     CDS             4940..5506
                     /locus_tag="PKZ74_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92573.1"
                     /translation="MTNDRRPTTDDLTTILAMGSERVVADLAVNVLEKTPEAFGDLLA
                     LCWLEQYPLSMRAARVAQLYCEKHPEAIYPFLDEAVEKTLKSKIGGVRRNFLKIFAEF
                     IDIQRISNQGHLLNTCFEWLLDARNTPAIRIHAMGLIYKMSLHEPDLLRELAATIEIV
                     MEEGETSLKTCGRKMLGRISRQSAVGSR"
     gene            complement(5549..>9982)
                     /locus_tag="PKZ74_07150"
     CDS             complement(5549..>9982)
                     /locus_tag="PKZ74_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92574.1"
                     /translation="DWSNITQQGTTQKLDAVPQVVMNPPQYRNFGSYETIVCCHTVDV
                     NATDRAGIRWYELRRTSGGNWTIRQQGTYSPDSHSRWMGSIMLNGYNEIALGYSIASS
                     TLYPGIRYCGQSAAAYASANSTMDVAESTIFTGTTYQPNYNRWGDYTSMQIDPEDDET
                     FWYVNQYSYYTGSGSYPYSRKTKIANFQIGNIILTANFVVSNPKPQTCSPVTFTDVSF
                     GSPASWSWSISPSSFHFINGTSSSSQNPQVQFTQAGSYSVTLIVSNGGTGDTIQKPDF
                     IEVLDCGFPTLPFTEDFSDGVIPPCWKNIDNSVNGHVWQFNNPGGWTINTSTGANGCA
                     ILDSYTYGNPVKKNADLITPLLDLSAYTSVNVFYEHYFRYFGPNSGWGRLYYSINDGA
                     TWTQLGSTISSSLNGADISIDATTQVAGQSRVRFKWNFIGYGYYWAIDDISITGTGPA
                     VWTGATSTDWATATNWSTGAVPTNATNVVIPKTATNWPTLSGDFTAGTTSDKITLYGE
                     AQMNVNGNVNIPAGETLTFTGDGQISLTGNWTNQGNFIPGQGSVRFTGGSPSTVTTSD
                     NSITNFQRSTFTKGMTDLVSGYSTGPSGNDAASNVNIGFTFNYLGTNYTQARICTNGW
                     MTLNLTGSTSATNADLFTTTAPSTTLAPWFDDLTVDNSTSFIRYKTEGIEPYRVFTVE
                     WKTLTTYRTTATARISFQVKLYETTNIIEFHYGTLTAGTHSGSESASAGLEDATGGPG
                     YFLEATTGSSTTGNSNLVSTTQWPLVNYRFSPPVQNFHHLVIDKTSSSLTLAQNSTIR
                     GNLEIAGGEFIGPGNVNKELRIYGDWINNGTFTQGAGTVMFSGSSAQTIGGNTATNFY
                     NLVFGNSAGFALQNNLTAGNIMTMTAGNIDCGADTLILGTSTSATGILNRTSGHIAGC
                     FKRWIAASTVTSVDFPTGAGNAYRNARITFTNNTGGSLTARFEPGDPGNNSGFPLTEG
                     GITINENNLYTEGSWTLTPTTLSSTNYALELTAEGFSTMGTPDETVRILKRTGDSDPW
                     MLNGTHVNGTATVAKRSGLSGFSRFILAKPCNFNISGNITYYNQANTPLTSGITVKLY
                     QNDTQLGTDYTVTGGTYQFTGLCPGTYQVRISSNRPVDGSINSTDAAQVNYWGVNSYE
                     IEKVRFYAGDVTGGTYYINSTDALRIQANFVNGTAFDRPAWTFWKAGETINSNSTPSE
                     SYASVTISGGDITANIYGLCTGDFNRSFIPGARNDGENQVEMIHDGEISVIPMQEFEL
                     PVRVASGCTVGAVSLAMILPSDLFIVKDIVMMNENGQAGWAFNGDELRVGWFTADPVQ
                     LAAGDKLLTLKLMMKDVAGILTNLRVSLSPGLLNELADENFIPVDHAVVVTDEISVKM
                     TGIGDTVESEMLTLSCYPNVTGEMTSIEYSIPRQGRVKLEISNIPGQKVELLADQVQK
                     KGNHKLSYDVTALQTGTYFVVLSYESSGRNMVKSALLVVSR"
BASE COUNT         2380 a         2360 c         2681 g         2561 t
ORIGIN      
        1 tgtgatccgg gaagagattg ctcctttatc ggcacgagaa atcaaaggtt tgaaatgggc
       61 cggggtcacg gtattcacgt ttatggtgct gatggcctgg gagcttatcc cggagaacgg
      121 ttttttgcgg ggacaggacg gaacagtgct ccattcgccg gttttgaagg gctttatctc
      181 ggtattgttc atcatggcgg ccgtatcagg gattgtgttc ggatatgtca gtggcacttt
      241 taagaagcat gatgatgtaa ttgccgggat gaacagcagt ttcaaaagcc tggtgtcttt
      301 cctggtcctg gtgttcttcg ccgcacagtt tgtggcgtgg ttcaagtgga gcaacctggg
      361 cctgctgatt gccgtaaaag gggccgacat tcttcagagc gcggatatcg ggctgatccc
      421 gctggtcatc ctttttgtca ttttatccgg gttcatcaac ctgttcatgg ggagtgcctc
      481 ggccaaatgg gccatcatgg gcccggtatt catcccgatg ttcatgctgc tggggtattc
      541 cccggagcta tcccaggcag tattccgtat cggcgacagt gtcacgaatg tgatctcgcc
      601 catgatgagc tttttcgccc tcatcatcgt ctattaccag aaatatgaac ccaaagcagg
      661 ccttgggacg ctgattgcga ccatgctgcc ctattccgtg acctttttcc tggcatggac
      721 cgccctgctg atcatttgga ttttatttgg tttacccctg gggcctgagg ccgggttgta
      781 ctactaaaag ggatggcgtc cctagggacg ccatcccttt ttttttagta tctttacacc
      841 ctgcataaat caaatcctaa agatatattg accatgaaag aaaaaagcat tgaattactg
      901 aacaaagccg ttgccgatga aatggccgcc gttcaccagt acatgtattt ccatttccgt
      961 tgcgacgacc tggggtattt cctcatctcc aacctattca agcgcactgc cattgaagag
     1021 atgatgcatg tggagaccct ggccgaacga atccttttcc tgaaaggaga agttgaaatg
     1081 aaagcagccc atgatgtgca gaaaatccat gatgtgaaac agatgctcga gctggccaca
     1141 aagatggaga ccgacagcgc gatcgactat aacaactggg ccatggaatg tgccaaaaac
     1201 gccgattccg ccacgaaaaa gatctttgaa gccctggtcg atgacgaaga acggcacttc
     1261 gaccaatacg acaaggaact ggataacctg gccaagtttg gcgataatta tctcgctttg
     1321 caatctatcg agaacagcaa gaacattgcc gctgcaggcg gaagaccggc tgaatagttc
     1381 aaagttcaaa gttcaaagtt caaagtgttt gtccggatgt cttgaaagat ggcattcggg
     1441 tactgtgtat tgtatgaatc gggcgggaaa atcaatgcat tttgtcccgg ttgatgccca
     1501 tttcgtcccg gggatagtcc tttcgtcaca aatccttcca ttcatcccaa attcctttac
     1561 aggcttccgg gatgagtgta atattgcccc atcaaatttt tattcattaa cctaaaaaac
     1621 aaaaaaatga aaaatgcaat tattatcgga gcgctggtga tccttggggc caccgggctt
     1681 attctgggat taagcggtta ccatgtgggt gaagcgggcg attttgccct tgggtatttc
     1741 tctgccggac aattctccgc cggcgtgttc tcggccggaa tgttttccgc agggatattt
     1801 tcagtagggt tattcagtgt gggcatcttt tcgctgagcg tattcaacgt ggctgtatat
     1861 ggcctgggct ttttcctgat cgcatggaag aagcattacg caaaagttaa agtggtggag
     1921 gaggagaaaa tataaatcct tttcgggcgt ttcatgataa gaatgattcc tgttttatcc
     1981 taaccggaaa atgatgacct acaccgagag ctatcacacg ctgaaatcta tcttttcaac
     2041 ttcacaaaaa gacaaaacat ataacggaac ggaaattcat gagtttccgt tcatttcttc
     2101 ctcctgcgga atcctgcccg ggaacccgga tcatttctgt gagaagcacc atcagcatct
     2161 ttccacttct ttttcgtttt ataacgatgt gggcaccatc cgggatgatg ttcatataaa
     2221 agacatagaa gtaaaagaaa accaagcttt taattataat ataatccggc cggccggcga
     2281 agggaaatgc cggaaggtga ttttcctgtt tcacggattt aacgaaaaag actggacaaa
     2341 gtacctgccc tgggcttatg cgctctgcag cgggacacag agcgccgtgg tgctgtttcc
     2401 catcgcgttt cacatgcaac gtgcgccgaa atactggagc agcaagcggg agatgttccg
     2461 gctcagcgaa gagcgcagga agcggtttcc ccacatcatc cactcgactt tgtcgaatgt
     2521 ggcgatcagc atgcggttgc attccatgcc gcaacggttt atctggtcgg gattgcagac
     2581 ctattacgat atgatccggt tcattgagga gtgcaggcag ggacgccatc ccgatatcga
     2641 cctgaacttt gaattcaata ttttcgcata cagcatcggc gggttcctgg cacagatact
     2701 gaagctgacc aatttcagcg gatacttcga cgatacgaaa gtttgcctgt tttgcggcgg
     2761 cgctgctttc aaccgcctgt cgcccgtgtc gaagttcatt ctcgacagtg aggcgaatgt
     2821 tgccctgtac tcgttcctgg tcgagcattt cgacaagatg ctggaccagg accatctgct
     2881 gcaccattat attcgtgacg accacccgga agggcgggta ttctatgcca tgctcgacta
     2941 ccggagatca agatcgttcc gcgaagagct tctgaaaaaa cacgaaaagt ggttctatgc
     3001 catttccctg agaaaagacg aggtcgtccc gtcgtttgag atcatcaaca ccctgcaggg
     3061 ggcttaccgc gacatcagaa tacgggtcga tgaggttgat ttcgaccgta aatatcttca
     3121 cgagaaccct ttcccggtga atgttgctta ctccgggcag gtcgatgaag attttgccct
     3181 ggtattcgat aaagcctgcc gtttcctggc ttgacaggta tctcattgaa tcttttgaga
     3241 acggggtgat gatgaccgga attttttcaa aaacagagat tataagtagc cacatctcaa
     3301 atttttttta aacatttggc cacttatatt gttcagaatt gataattagt tggttacatt
     3361 tcttaaaaca ataaaatggc cttttatcaa ctcactaaaa aacaattcgt cccggcttct
     3421 atcgacgaag tctgggattt catctcatct cctaaaaacc tgaaagagat cacccccgaa
     3481 tacatgggat tcgacatcac ttctgaaaac ctcccggaaa aaatgtatgc cgggatgatc
     3541 atcagttata aagtcaggcc gctgatgagg attccgatga cgtgggtgac ggagatttca
     3601 caggtggttg ataagcaata ttttgtcgat gaacagcgtt ccggtcccta tgctttctgg
     3661 caccaccagc atctcattga gccttatgag aacggggtga tgatgaccga tatcgtttcg
     3721 tacaagccgc cgctgggatt tctcgggagc attgccaatg ctttgatgat caggaaacag
     3781 ctggaaggga tctttgctta ccgcgaaaaa gccttgaaag aaaggtttcc ctgaaagctt
     3841 taacaaaaac cactaagccc aacaccgaac atcttatatt tgtctccagg atttgagtat
     3901 tttccggctt tgttttattt tggcggaaaa aaagaaaaga aaaaaggaaa taattttatg
     3961 atgaacggtg atattgattc gggaaagaga tcttttgaat tgcggtctct cggtgctacc
     4021 ggtttcgata ctgtttacaa agccttcagc ctggcttttc aggattacga gatccggctg
     4081 aaccgttcac agttaatggc aatgttgcac aggcgcggtt tcgatcccgg gttgtcgttt
     4141 gccgcgttcg acggagatga cattacggcc tttaccctga acggcatcgg gactttcaac
     4201 gggatcccga cggcctatga tacggggacg ggaacgctca aggagttcag gggacagggg
     4261 ctggctacga agatcttcga atattccatc ccatttttga aagaaaaggg cgttgaacaa
     4321 tacctgctgg aggttctgca gcacaacaca ggggcggtat cggtgtataa gaatcttgga
     4381 tttgaagtga cccgcgagtt taactatttc attaagaata tgaatgaaat cagggatgag
     4441 attttaactc aggatattcc cttttccata cgacaaacgg gattagatga attaaaggcg
     4501 gcttcggaat tttgggattt tcatccatcg tggcagaacg gttttgaatc cataaacaga
     4561 tcggccggcg aatttgtttg cctgggagct ttccatgaac accgtctggc agggtattgc
     4621 atttttgagc cccttacggg ggatgtgacc cagattgcgg tcagcaggcc atacaggcga
     4681 aaggggatcg gttcattcct gctccgggaa atgatcgggc gtaaccggag tgaagtcctg
     4741 aaaattatca atacggaaat ctcctgtgaa tccataaccc ggttcttagc ctccagaaat
     4801 attgaaccca caggccggca atttgaaatg atcagaaagc tttgatcttc tttgcttgat
     4861 aacaggaata gcgatcatat ccggcgaaca acaaccaatt cttcaggtat ctccttaaaa
     4921 aacaaccgac cagcaatcca tgactaacga ccgacgaccg acgaccgacg accttaccac
     4981 cattcttgca atgggctccg aacgggtggt ggccgacctg gctgtgaatg tgctggagaa
     5041 aacaccggaa gctttcggcg acctgctggc gctatgctgg ctggagcagt acccgctgtc
     5101 gatgcgggcg gcgcgcgtag cccagcttta ctgcgaaaag cacccggagg ccatctatcc
     5161 tttcctggat gaagcggtgg agaaaacgct gaagtcaaag atcggcgggg tccgccgcaa
     5221 ctttctgaag atatttgccg aattcatcga tattcagcga atcagcaacc agggacacct
     5281 gctgaatacc tgcttcgaat ggctgctcga tgcccgcaat acgccggcta tccggatcca
     5341 tgccatggga ctgatctaca aaatgagcct ccatgaaccc gacctgctgc gggaactggc
     5401 cgcaacaata gagatcgtca tggaagaagg agagacctcg ctgaagacgt gtgggaggaa
     5461 gatgctgggg aggatcagtc ggcagtcggc agtcggtagc aggtagtcgg gcagttggac
     5521 aaaggaggtg gtgactaacg gccgacgact aacgactaac gaccagcaaa gctgacttca
     5581 ccatgttccg gccgctgctt tcgtacgaaa gtacaacgaa gtaagtcccc gtttgaagcg
     5641 ccgttacatc atatgacaat ttgtgattcc cctttttttg aacctggtcg gcaagcaact
     5701 ccactttctg accgggaata ttgctgattt ccagctttac cctgccctgc cggggaattg
     5761 agtattcaat ggaggtcatt tctccggtca cgttcgggta acatgaaagg gttaacattt
     5821 ctgactcaac cgtatcgccg attccagtca ttttcactga aatctcatcg gtaaccacga
     5881 ccgcatgatc gaccggaata aaattctcat cggcaagctc gttcaggaga ccgggagaca
     5941 gggagacccg gagattggta agaatgccgg cgacatcctt catcatcagt ttgagggtaa
     6001 gaagcttatc gccggcagcc agttgcaccg gatcggccgt gaaccacccg acccgcaatt
     6061 catcgccgtt gaacgcccag ccggcctggc cattttcatt catcatgaca atgtctttca
     6121 caataaaaag atcagaaggc agaatcatag ccaatgaaac tgccccgacc gtgcatccgg
     6181 aggcgacccg caccggcagc tcaaattcct gcatgggtat aacggatatt tcgccgtcgt
     6241 ggatcatttc tacctggttt tcgccatcat ttcttgcacc gggaataaaa ctgcggttga
     6301 agtcgccggt acacaagccg tagatgtttg cggtgatatc gccgccggag atggtcacgc
     6361 tggcatagga ttccgaaggg gtgctgttgc tgttgatggt ttcgccggct ttccagaagg
     6421 tccaggcggg gcggtcgaac gcggtaccgt tcacaaagtt cgcctggatg cgaagcgcgt
     6481 cggtgctgtt gatgtagtaa gtgccgcccg tgacatcgcc ggcgtaaaac ctgacttttt
     6541 cgatttcgta agaattgacg ccccagtaat tgacctgtgc cgcgtcggtg ctgttgatgc
     6601 tcccgtcaac cggccggttc gacgagatcc tgacctggta tgtgcccggg cacaggccgg
     6661 tgaactggta ggttccgccg gtaacggtat aatcggtacc cagctgggta tcgttctggt
     6721 aaagttttac ggtaatcccc gatgtgagcg gggtattggc ctggttgtag taagtgatat
     6781 ttccggaaat attgaaatta cagggcttgg cgaggatgaa ccgggaaaaa ccgctgaggc
     6841 cggagcgttt ggccaccgta gcagttccgt tcacatgggt cccgttgagc atccaggggt
     6901 cagaatctcc tgtccgcttc agaatcctga ccgtttcatc gggggtcccc atggttgaaa
     6961 acccttcggc ggtgagctcc agggcgtagt ttgtgctgga aagcgttgtg ggagtgaggg
     7021 tccaggatcc ttcggtgtag aggttgtttt cgttgatcgt gatacctcct tcggttaatg
     7081 gaaatccgct gttgttgccc ggatcgccgg gttcgaaccg ggctgtgagc gaaccgccgg
     7141 tgttattggt gaaggtgatc ctggcattgc ggtaagcgtt gccagccccg gttgggaaat
     7201 ccaccgatgt taccgtggag gcggcaatcc atcttttgaa gcatcccgcg atatggcctg
     7261 aggtccggtt caatatgccg gttgctgaag ttgaagtccc gagtatcaaa gtatcggcgc
     7321 cgcagtcgat attgccggcc gtcatggtca tgatattgcc tgcagtaagg ttgttttgaa
     7381 gcgcgaagcc ggctgaattt ccgaaaacaa ggttgtaaaa gttcgtggct gtgttaccgc
     7441 cgatggtttg agcagacgag ccggaaaaca ttaccgtacc ggcgccctgc gtaaatgtgc
     7501 cgttattgat ccagtctcca tatatccgca actccttgtt cacattgccg ggtccgatga
     7561 attctccgcc ggcaatctcc aggttccccc tgatggtcga attctgggcc agggtgaggg
     7621 atgaactggt tttatcgatg accaggtgat ggaaattctg taccggcggg gagaaacggt
     7681 aattgaccag cggccattgg gtggtgctga ccaggttgga attgcctgta gtgctggaac
     7741 cggtagtggc ttcgaggaaa tatccgggcc cgccggtagc gtcttccagt ccggccgatg
     7801 cgctttcact tccgctgtgc gtccctgcag tcagggtacc gtaatggaat tcgatgatat
     7861 tcgtggtttc atacaatttt acctggaagc tgatccgggc agttgcagtg gtcctgtagg
     7921 tggtcagcgt tttccattcc actgtgaaga cccggtaggg ttcaatccct tccgttttat
     7981 aacggatgaa agatgtggag ttatccacgg tgaggtcgtc gaaccagggg gcaagggtgg
     8041 tggatggggc tgttgtcgtg aataaatctg catttgtcgc tgaggtcgag ccggtcaggt
     8101 tcagtgtcat ccagccgttg gtgcagatcc gcgcctgggt ataatttgta ccgagataat
     8161 tgaaggtaaa accgatattt acattagatg ctgcatcgtt acccgagggt cccgtcgaat
     8221 aacctgaaac caggtcggtc atcccttttg tgaaagtgct cctctggaag ttagtaatgg
     8281 aattatcgct tgtggttacg gtagatggcg aaccgccggt gaatcgtacg ctcccctgcc
     8341 cggggatgaa atttccctgg tttgtccagt ttccggtcag gctgatctgg ccatcgccgg
     8401 taaaagtcag cgtttcccct gccgggatgt tcacattgcc gttgacattc atttgggctt
     8461 caccgtagag ggtgatttta tcgctggtgg tgccggctgt aaagtcgccc gaaagcgtcg
     8521 gccagttcgt tgccgttttt ggaatgacga catttgttgc gtttgtaggt acagccccgg
     8581 tactccagtt ggtggcggta gcccagtcgg tggaggtggc gccggtccac actgccggcc
     8641 ccgtgccggt gatgctgata tcgtcgatgg cccagtaata accataacca atgaaattcc
     8701 atttgaaccg cacccgggat tgccccgcga cttgtgtcgt tgcatcaatg ctgatatcgg
     8761 cgccattaag tgaactggaa attgtactgc ctagctgcgt ccatgtagcg ccatcgttaa
     8821 tgctgtagta taaacgcccc caacccgagt ttgggccaaa atatctaaaa tagtgctcat
     8881 aaaatacatt gaccgaagta taagctgaca agtcaagcaa tggtgtgatc aggtcggcat
     8941 tctttttcac cggatttccg taagtataac tatccaggat ggcacaacca ttggctcccg
     9001 tcgacgtatt gatcgtccag ccccctggat tattgaattg ccacacatgc ccgttgaccg
     9061 agttgtcgat atttttccag caggggggga tgacgccgtc ggagaagtct tcggtaaacg
     9121 ggagggttgg aaacccgcag tctaaaacct ctataaaatc aggcttctgg atcgtatcac
     9181 ccgtaccgcc gttactcacg atcagggtta cggaataaga tccggcctgg gtgaactgca
     9241 cctgggggtt ttgtgaggag gaggaggttc cgttgataaa atgaaaggaa gacggggaaa
     9301 tggaccagct ccaggatgcc gggctgccga agctgacatc ggtgaaagtc accggggaac
     9361 aggtttgagg tttgggatta ctgacaacaa aatttgccgt cagtatgata ttgccgatct
     9421 ggaagttggc aattttggtt tttcgtgaat aaggatacga tcccgagccg gtgtagtaag
     9481 aatactggtt gacataccag aaggtttcat cgtcttccgg gtcgatctgc atggaggtat
     9541 aatcgcccca gcggttgtaa ttcggctggt aggttgttcc tgtaaatatg gtggattcgg
     9601 ctacatccat ggtgctgttg gcgctggcat aagctgccgc cgattgtccg caatatctga
     9661 tccccggata gagtgtggat gaagcaatcg agtatccgag ggcgatttcg ttgtagccgt
     9721 tcagcattat tgaacccatc catcgcgagt ggctgtcggg cgaataggtc ccctgctggc
     9781 gtatggtcca gttgcctccg gaggtacgcc tgagttcata ccatctgatc ccggcccggt
     9841 cggtagcatt gacgtcgacg gtatggcagc agacgatggt ttcatacgat ccgaagttgc
     9901 ggtattgcgg cgggttcatc accacctgcg gaacggcgtc gagtttctgc gtggtcccct
     9961 gctgggtgat attggaccag tc
//
LOCUS       DAOPNH010000109         9929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7387_length_9929_cov_7.625481, whole genome shotgun sequence.
ACCESSION   DAOPNH010000109 DAOPNH010000000
VERSION     DAOPNH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_9929_cov_7.625481"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKZ74_07155"
     CDS             <1..531
                     /locus_tag="PKZ74_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92575.1"
                     /translation="AGNLILFLIIIAFITLAFIKRINVYEAFIDGAKDGFSVAVKIIP
                     YLVAILVAVGVFRTSGAMDYLVGGIKDIVAFLGADTGFVDALPTALMKPLSGSGARGM
                     MVETMKQYGADSFAGRLSCIFQGAADTTFYIVAVYFGSVSIRKTRYAITAGLIADLAG
                     VIAAIFVAYLFFSQKA"
     gene            complement(538..1812)
                     /locus_tag="PKZ74_07160"
     CDS             complement(538..1812)
                     /locus_tag="PKZ74_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92576.1"
                     /translation="MKRFWATIKKEFLVLVRDWAGLGLIFLMPAVLVLVMTLIQDASF
                     RKLDETKITLLYLDEDLDSLGSHVKTGLLEADYFEVVTEVDGKILTEKEILSQVAAGN
                     YQIGIRIPSGSTDAIRLRGQQMVQQTILGIEAPPSDSSGQARILIYYDPAMKNSFRLT
                     VRNALENFTSKIEAGIMFKALSDEMAQYLPEPPAPMTGAGSSISYEEIYATDTHTEII
                     PNSVQHNVPAWTIFAMFFIIIPLTGNLIKERESGLAMRLMTMPGNQFQSLGAKVFIYT
                     IVGMSQFALMLILGKELLPLFNLPPLILGPEKTALILMALTTTLAATGYSVLVGTIAR
                     THDQAAVFGMVSVIILAAIGGIWVPVFMMPNLMQAASQVSPLNWSLDGFYQILLRGGG
                     IREVAENAGALFLFFAVTLTLAVAISTKSRRA"
     gene            complement(1812..2567)
                     /locus_tag="PKZ74_07165"
     CDS             complement(1812..2567)
                     /locus_tag="PKZ74_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92577.1"
                     /translation="MIPTPAIEINHLAKTYRGADEPALDGISLTVPEGEIFGLLGPNG
                     AGKTTTINILCGILQPSGGNIRILGKDSIREHDAIKHIIGVVPQDIALYPSLTARENL
                     KFIGSMYGLSGKNLADRVDECLHVFGLTKFGGRMVGKYSGGMKRRVNLIAGILHQPRI
                     LFLDEPTVGIDVQSRTVIIEYLKKLNEDGTTIIYTSHYMEEAELLCSSIAIIDRGKII
                     TQGRPASMVGSQPGFTNLESIFLQLTGRKLRDY"
     gene            complement(2567..3586)
                     /locus_tag="PKZ74_07170"
     CDS             complement(2567..3586)
                     /locus_tag="PKZ74_07170"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPM92578.1"
                     /translation="MNQNKYYIDFQHKPAAHCENGATSNLLNHYNIRLSEPMVFGIGS
                     GLFFSYMPFMKLNGLPVVSFRPWPGTIFNRNTRRLGIEIQRYKFRDRKEAMKMLDENL
                     AKGIPTGCLVGVYHLTYFPPAYRFHFNAHNIVVIGRENGEYLVSDPIMPGIERISYHD
                     LERVRFAQGTQPPKGRMYFIKGISETFDLKNAIVEGIKQVCKDMLEIPIPLFGVKGIR
                     YMAKQMRKWPKKLGERKASLYMGQVVRMLEEIGTGGAGFRFIYAAFLQEAARELNQPW
                     LNEISDEMTQAGDRWREFAIIAGRIFKSRAGQEENYQAAADVLLDIAGREEKIFLKLR
                     HIKLS"
     gene            complement(3573..3998)
                     /locus_tag="PKZ74_07175"
     CDS             complement(3573..3998)
                     /locus_tag="PKZ74_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92579.1"
                     /translation="MKKKEVPQDEGLSDGLFEDICYALDDDGHYVVVPTTGFQPKTDA
                     LLQAWDVIHEKIEEVRQLVLAGKLSPIAYFMEKNIMDARLLADYMGIPKWKVKRHLKP
                     KHFNKLDDDILDRYAETFNINREQLKDISVIQEDKNESK"
     gene            complement(3995..4441)
                     /locus_tag="PKZ74_07180"
     CDS             complement(3995..4441)
                     /locus_tag="PKZ74_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92580.1"
                     /translation="MLVPKEKIMELLPQRPPMVMVDELVMCNEMEAVSRLFIRDDNIF
                     LGETGFSPSGMLEAMAQTAAARTGYLLKKQPESAHKSIPVGVIGSIKNFKLHFQPGSG
                     DTIFTTVFVEHEVLQATVIRAKVEVEGRLSAESTLQIFLSEEQAKP"
     gene            complement(4487..5626)
                     /locus_tag="PKZ74_07185"
     CDS             complement(4487..5626)
                     /locus_tag="PKZ74_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPM92581.1"
                     /translation="MINEVYITRLSKFLPNKPVDNDRMEDFLGMIDGKPSKAKRIILR
                     NNQITQRYYALNENGKLTQNNAQLTAEAIRQLFDKDFTIEEIELLACGTTSPDQTLPS
                     HASMVHGELGGKPLEIISPSGSCATGMHAMKYGYLAVKAGEKKNAVCSGSELFSIMMQ
                     ADKFEKETENLHLLDQNPILAFEKEFLRWMLSDGAGAALLQNRPNPDGLSLRIDWIES
                     SSFAGRLETCMYSGGDKLPDGRIKGWKEFDTQDILNKSLFSLRQDVKLLGENIYQTCN
                     MFLADIVKRRKLDLNQIDYFLPHISSEYFRYRIDADSKEKGVHIPQEKWFTNLVTLGN
                     VGAASIYFMLEEIFHSGTLKKGQKLLLGVPESARFSYVYSLVTVV"
     gene            complement(5787..6698)
                     /locus_tag="PKZ74_07190"
     CDS             complement(5787..6698)
                     /locus_tag="PKZ74_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92582.1"
                     /translation="MKKILIIYYTQTGQLRQIVDSITEPLKNHCLLFFEELRPVPSYP
                     FPWNGMPFFQVFPESVREIPCELEPLANNPDLDYNLVILAYQVWYLSPSIPMTSFLKS
                     EAAGKILKGKPVITVLGVRNMWIMAQERVKAMIAGAGGKYCGNIVMADPAGNLTSVIT
                     IVRWMMKGEKTPFRWMGVNFPAAGVPYEEIGRGREYGRLIMHYLISSFTGELQEDLVR
                     QGAVKVNPVLYKIEKTGKRIFGIWSKLVLAKGGYNDPAREGRLKAFQYYLFAVIYLVS
                     PIVAAVNRFLFFLNPGAARKIVDRFSR"
     gene            complement(6816..7700)
                     /locus_tag="PKZ74_07195"
     CDS             complement(6816..7700)
                     /locus_tag="PKZ74_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM92583.1"
                     /translation="MAGWQGKSRGTLLGHRIFVFILKYLGLSPAYLLLFFVASYYFLF
                     SRKSNRFIYRYFRDVHRYPPSKARRAVFRNYYIFGQILIDKVAIRSGLEDKYTFDLAD
                     EVHLAGMENGGFMISAHIGNWEMSGKKSDRIHKTMNLVMLDAEHRRIKHYLETVMKDR
                     NFNIIPLGDDLSHLISIRQALDRGELIAIHGDRFMPGMKTVRATFLGKEAGFPEGPFY
                     LALRMNAPVSFAFAMKAGRKHYHFHATPPKKYAIRPGKRISQEDIEPVLQDYVSALET
                     MLKKYPEQWFNYYDFWID"
     gene            complement(7703..7951)
                     /locus_tag="PKZ74_07200"
     CDS             complement(7703..7951)
                     /locus_tag="PKZ74_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM92584.1"
                     /translation="MEKSKIIGEINGFLVEEFEIEEHLIRPEASWKEIGIDSLDFVDI
                     VVIIEKNYGFKLKGEEMASIKTLGQFYDYIFSRIQNQN"
     gene            8337..8636
                     /locus_tag="PKZ74_07205"
     CDS             8337..8636
                     /locus_tag="PKZ74_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM92585.1"
                     /translation="MNELTFGEYLRLRREELSLPLRKVASYLDIDTSTLSKVERGERP
                     ASKDYLKPLAEILNLDLKDIQTKYIADKITKDFEGLEHVQSGLKAAENMLNQKRK"
     gene            8636..9898
                     /locus_tag="PKZ74_07210"
     CDS             8636..9898
                     /locus_tag="PKZ74_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPM92586.1"
                     /translation="MPEIFDNIENYLVDGLRYTLDVSNRADFCVGYFNLRGWKQVAEN
                     IEPWNGIEGNCCRLLVGMQKPPLEIIKEYFSTSDQLTIDNQKAAAIKKKLAQEFKEQI
                     TVGVPTDEDERGLRQLSSQIKAKKVIVKLFLRYPLHAKLYLCFRDDKINPIIGYLGSS
                     NLTLSGLSLQGELNIDVLDRDASGKLSKWFNDRWEDKFCIDISEELTEIIDNSWAADK
                     LIHPYHIYLKIAYHLAREARAGLAEFKVSKIFEKQLLDFQMKAVLVAAHHLNKRGGVI
                     IGDVVGLGKTITAAALAKMFEDDYLLETLIICPKNLVKMWEDYRHKYQLRAKIIPSSQ
                     VQTILPKERRYRIVVIDESHNLRNREGKRYRSIQEYIHLNDSKVIMLTATPYNKTYLD
                     LSNQLRLFIPEEKDLGISPERFKVLPIF"
BASE COUNT         2490 a         2269 c         2493 g         2677 t
ORIGIN      
        1 gccgggaacc tcatcctgtt tctgatcatt atcgccttca taacgcttgc attcatcaaa
       61 cggatcaatg tttacgaagc atttatagat ggggcgaagg atggtttttc ggttgccgtg
      121 aagatcatac cttaccttgt ggccatcctg gtggctgtcg gggtattcag aacctccggt
      181 gcgatggact acctggttgg cgggatcaaa gacattgtgg cctttcttgg agccgacacc
      241 ggctttgtgg atgcgcttcc tacagctctt atgaagccgc tgagcggttc cggagcccgg
      301 gggatgatgg tggagacgat gaagcagtac ggcgccgatt cgtttgcggg ccgcctctcc
      361 tgtattttcc agggcgctgc agatacaacc ttttatattg ttgccgtcta cttcggctcc
      421 gtcagtatcc gcaagacacg ttatgcgata acggccgggc tcatcgccga cctggcgggt
      481 gtaattgccg cgatcttcgt agcttacctg ttcttttccc agaaggcctg acagatatca
      541 ggcccttcgg gatttggttg atatagccac cgccagggta agggtaacag cgaagaatag
      601 aaagagtgcg ccggcatttt ctgcgacctc cctgatcccg ccgccgcgga ggagtatctg
      661 gtagaatccg tcgaggctcc agttgagcgg ggagacctgg ctggcagctt gcataaggtt
      721 gggcatcatg aataccggca cccagatccc gccgatggcg gccaggatga tcaccgatac
      781 cattccgaag acggctgcct ggtcgtgtgt ccgggcaata gtaccgacca gaacactgta
      841 gccggtagct gccagggttg ttgtcaaagc catcaggatg agggcggttt tttcaggccc
      901 caggatgagc gggggtagat tgaaaagggg cagcaactct tttcccagga tgagcatcag
      961 ggcgaattgt gacatgccga cgatcgtata gatgaagacc ttggcgccga gcgactgaaa
     1021 ctggttgccg ggcatggtca taagccgcat ggccagtccg ctctcgcgtt cctttatcag
     1081 gttgccggtc agggggatga tgatgaaaaa catggcaaag atggtccagg cagggacatt
     1141 atgctggact gagttaggga tgatctcggt gtgggtatcg gtggcataaa tctcttcata
     1201 actgatgctg cttccggcgc cggtcatagg ggcaggcggt tcgggcaggt attgcgccat
     1261 ttcatcggag agggccttga acatgatccc ggcctctatt ttcgaggtga aattttccaa
     1321 cgcattcctg acggtcagcc ggaatgagtt tttcatggcc ggatcatagt aaataaggat
     1381 gcgggcctgc cctgatgaat cggaaggagg tgcctcgatc ccaaggatgg tctgttgaac
     1441 catttgctgt ccgcgcaacc tgatagcatc ggtcgagcca gacggaatcc tgatcccgat
     1501 ctggtagtta ccggcagcga cctggctgag tatctctttt tcagtcagga tcttcccatc
     1561 gacctcggtc accacctcga aataatcggc ttccagaaga cctgttttta catgagatcc
     1621 aagggagtcg aggtcttcat caagatacag cagggtgatc ttcgtctcgt cgagcttccg
     1681 gaaagaagca tcctggatca gtgtcatgac tagcaccagg acagccggca tgaggaagat
     1741 caggccaaga ccggcccagt cgcgcaccag aacgagaaat tcctttttta tagtagccca
     1801 gaatcttttc atcagtaatc cctcagtttt cgtccggtca gttgaaggaa aatgctttcc
     1861 aggttagtaa acccgggctg gcttccgacc attgatgccg gccggccctg ggtaatgatc
     1921 ttgcccctgt cgatgatagc aatggaactg cacaataatt cggcttcttc catatagtgc
     1981 gatgtatata tgatcgtcgt cccgtcttca ttcagttttt tgagatattc gataataacg
     2041 gtcctggact ggacgtcgat cccgacggtt ggttcatcga ggaagagaat ccggggttga
     2101 tgaaggatac cggcgatgag attcacccgt cgtttcattc cgcccgaata tttcccgacc
     2161 atgcgtccgc cgaatttggt cagaccaaaa acatggaggc attcatccac ccggtcggcc
     2221 aggttcttcc ccgaaagccc gtacatgctt ccgatgaatt tgaggttttc ccgggctgtc
     2281 agtgaagggt agagggcgat atcctgcgga actacgccga taatatgttt tatcgcatca
     2341 tgttcgcgga tggagtcttt tccaagaatt ctgatattgc cgcctgacgg ttgcaggatt
     2401 ccgcaaagga tgttgatggt cgtggttttg ccggcgccgt tcggaccgag cagaccgaag
     2461 atttcccctt cgggcacggt caggctgatc ccgtcaaggg ccggttcatc cgctccccgg
     2521 taggtcttgg ccagatggtt tatctctatg gctggcgttg ggatcatcac gacaatttga
     2581 tatggcgcaa tttaaggaaa atcttttctt ccctgccggc aatgtcgagc aggacgtcgg
     2641 cggctgcctg gtaattctct tcctgcccgg cgcggctttt gaagattctg ccggcaatga
     2701 tggcgaattc gcgccaccgg tcgccggctt gtgtcatttc atccgatatt tcattcagcc
     2761 agggctggtt cagttcacgg gcagcttcct ggaggaatgc agcataaata aaccggaatc
     2821 cggcgcctcc tgtaccgatc tcttccagca tcctgactac ctggcccata tagagcgagg
     2881 ctttcctttc tcccagtttc tttggccatt tccgcatttg ctttgccatg taacggatcc
     2941 ctttaacgcc aaaaaggggg atcggaatct cgagcatatc tttgcaaacc tgtttaatac
     3001 cttcaacgat tgcatttttc aagtcaaaag tttcggaaat ccctttgata aagtacatac
     3061 ggcctttggg tggctgcgtc ccctgggcaa acctgaccct ttcgagatcg tggtagctga
     3121 tccgctcgat tccgggcatg atcgggtcac tgacgagata ttcaccgttt tccctaccaa
     3181 tgacaacgat attgtgggcg ttaaaatgga accggtaagc aggagggaag tatgtgagat
     3241 ggtacacccc gacgaggcat ccggtcggaa tgcctttagc caggttctca tccagcattt
     3301 tcatcgcttc tttcctgtct ctgaatttat aacgctgaat ttcaataccg agcctgcggg
     3361 tattgcgatt aaagatggtt cccggccagg gcctgaagga tacaacgggg agaccgttaa
     3421 gtttcataaa aggcatgtac gagaaaaaaa gtcctgagcc gatgccgaaa accattggtt
     3481 cactgagacg gatgttgtaa tgattcagca ggttggaggt ggctccgttc tcgcagtgtg
     3541 cagccggctt atgttgaaaa tcgatatagt atttattttg attcattttt atcctcctgt
     3601 ataacgctga tatcctttaa ctgttccctg tttatattga atgtttccgc atagcggtca
     3661 aggatatcgt catcaagctt gttaaaatgc ttgggcttca gatgccgctt caccttccat
     3721 ttcgggatac ccatataatc ggccagcaac cgggcatcca taatgttttt ctccatgaaa
     3781 taagcgatag ggctaagttt tccggccagt accagttgcc ttacctcttc gattttttca
     3841 tggattacat cccacgcctg aagcagtgca tcggtctttg gttgaaaacc ggtagtcggg
     3901 acaacgacat aatgcccatc gtcgtccagc gcatagcaga tgtcttcaaa caaaccgtcc
     3961 gacagacctt cgtcctgggg aacttctttc ttcttcatgg tttagcctgt tcttccgata
     4021 aaaaaatctg caaagtgctt tcggcggaga gcctgccctc cacctccact ttcgccctga
     4081 tcaccgttgc ctgtaaaact tcatgttcca cgaaaaccgt agtaaaaata gtatcacctg
     4141 atccaggttg aaaatgaagc ttaaaattct ttatactccc gataacaccg acaggtatac
     4201 ttttgtgtgc gctctccggc tgttttttca acaggtagcc cgttctggcg gcggctgttt
     4261 gagccatggc ttcgagcatc ccggaaggtg aaaaaccggt ttctcctaaa aaaatgttgt
     4321 catcacggat aaaaagcctg gagaccgctt ccatttcatt gcacattacc agttcgtcca
     4381 ccataaccat tggggggcgc tggggcagca attccataat cttttctttc ggaaccagca
     4441 tcagggcggc tcattttcaa agtgcgggca aagataataa aagaagtcaa acgacagtta
     4501 ccaggctgta aacataagag aaccgggcgc tttcgggaac gccgagaagc agtttctgcc
     4561 cttttttcaa agttccggaa tggaagatct cttcgagcat gaagtagatg gaagcggcgc
     4621 cgacattgcc cagggtgacc aggttggtaa accatttttc ctgcgggatg tggactcctt
     4681 tttctttact gtcggcatcg atacggtaac gaaaatattc ggatgatata tgcggcagga
     4741 aataatcgat ttggttgagg tcgagttttc ttcttttcac gatgtcggcc aggaacatat
     4801 tgcaggtctg gtagatgttt tcgccgagca gtttcacgtc ctgcctgaga gaaaagaggg
     4861 atttgttgag gatatcctgc gtgtcgaact ccttccatcc tttgatccgt ccgtcaggca
     4921 gtttatcacc gccgctgtac atgcaggttt ccagccggcc ggcaaaggag gatgattcaa
     4981 tccagtcgat cctgagcgag aggccatccg gattgggcct gttctgcagc aacgcagcgc
     5041 cggcgccgtc gctaagcatc cagcggagga actctttttc gaaggccagg atgggatttt
     5101 gatccagcag gtggagattt tcggtctctt tttcaaattt gtctgcctgc atcatgatag
     5161 agaagagctc tgaaccggaa catacggcat ttttcttctc accggccttc acagccaggt
     5221 aaccgtattt catggcatgc ataccggtgg cacaggagcc ggagggggag atgatctcca
     5281 gcggtttgcc tcccagttcc ccgtgcacca ttgaagcgtg cgaagggagc gtttgatcgg
     5341 gagatgtggt gccgcaggcc agaagctcta tttcttcaat agtaaaatct ttatcaaaaa
     5401 gctgacggat ggcttctgcg gtaagttgtg cattgttttg ggtcagtttg ccattttcat
     5461 taagcgcata gtagcgctgg gtgatctggt tgtttctcag gatgatccgt tttgctttcg
     5521 acggtttccc gtcgatcatt cccaggaaat cttccatccg gtcgttatcc acaggtttat
     5581 tgggcaggaa tttggagagt cgggtgatgt aaacctcatt gatcatatac aagccgcatc
     5641 aaagtatttt gagtgggcaa agttaaaatt ttccgggaga ttttaaatag tgagactcca
     5701 tctcgatttt tgagatggag tctccaatga aacgagacgc catctggcca attgagatgg
     5761 agtctcatgg aattcgagat gccatctcac ctggagaatc ggtcaacaat ctttctggca
     5821 gcgccgggat tcaaaaaaaa caggaagcgg tttacggcgg caacgatggg cgagaccagg
     5881 taaataaccg caaaaaggta gtattggaat gctttcagcc tcccttcgcg ggccggatcg
     5941 ttgtagccgc ctttggccag aaccagcttc gaccagatcc cgaagatccg tttgccggtc
     6001 ttttcaatct tgtacaacac cggattaact tttaccgcac cttgccggac aagatcctcc
     6061 tgcaattctc ctgtgaagct acttattaaa tagtgcataa tcaatcttcc atattctctc
     6121 cctctcccta tctcctcata cggtacaccg gcagccggaa agttcactcc catccagcgg
     6181 aacggcgttt tttccccttt catcatccac ctgacgatgg tgatcacgct ggtcaggttg
     6241 ccggccggat cagccatcac tatgtttccg cagtattttc ctccggcccc ggcaatcatc
     6301 gccttcaccc tctcctgcgc catgatccac atgttcctca cccccaggac ggtgataacc
     6361 ggtttgccct tcagtatctt acctgcggct tcacttttca gaaaggaggt catcggtata
     6421 gatggtgaaa gataccagac ctggtaggcc aggatcacca ggttgtaatc aaggtccggg
     6481 ttgttggcca acggttcgag ctcgcagggg atctccctga ccgattccgg gaagacctgg
     6541 aagaaaggca ttccgttcca ggggaaagga taggagggga cgggcctgag ttcttcgaaa
     6601 aaaagcaggc agtggttctt caacggttca gtgatcgagt cgacgatttg ccttaactgg
     6661 ccagtttggg tatagtaaat tatcagtatt ttcttcattt tctaaccgaa atgggattca
     6721 aaattaaata tatccgcgaa gattcgtaaa attcgcatca tccgtgttct aataagagaa
     6781 cgcgaataat cacggatgca acgaattttc acaaattaat caatccagaa atcgtagtaa
     6841 ttgaaccact gctccgggta cttcttcagc atcgtctcca gtgcagaaac ataatcctgc
     6901 agtaccggtt cgatatcctc ctgcgaaatt cttttgcctg gccgaattgc atattttttc
     6961 ggaggtgtgg cgtggaagtg ataatgtttc cggccggctt tcatggcgaa cgcaaagctg
     7021 accggggcat tcatgcgcaa cgccaggtaa aaagggcctt cggggaaacc ggcttccttc
     7081 ccgagaaacg ttgccctgac ggttttcatc cccggcatga aacggtcgcc gtggattgcg
     7141 atcagttcgc cgcggtcgag cgcctggcgg atggagatca ggtgcgaaag gtcgtcgccg
     7201 agcgggatga tattaaagtt tctgtctttc atgaccgttt cgaggtagtg cttgatgcgc
     7261 cggtgttcgg cgtcgagcat caccaggttc atggttttat ggatacggtc cgatttcttg
     7321 cccgacatct cccagttgcc gatgtgtgcg ctgatcataa acccgccgtt ttccataccg
     7381 gccaggtgca cttcgtcggc caggtcgaag gtgtacttgt cctccaggcc ggagcggatg
     7441 gcgactttat cgatgaggat ctgtccgaag atatagtagt tacggaatac ggcgcgacgg
     7501 gcttttgaag gggggtaacg gtgaacatcc ctgaaatagc ggtaaatgaa gcggtttgat
     7561 ttacgggaga agaggaagta ataagaagcc acgaagaaaa gcagaagata ggcgggtgat
     7621 aatccgaggt atttcaggat aaagacgaat atcctgtggc ccagaagggt gccgcgggat
     7681 ttgccctgcc acccggccat ggctagttct ggttttggat gcgggagaaa atgtaatcgt
     7741 aaaactggcc gagtgttttg atgctggcca tctcttcacc cttcagctta aagccgtaat
     7801 tcttttcgat gatcaccacg atgtccacga aatcgaggct atcgatgccg atctctttcc
     7861 aggaagcttc cggtctgatc agatgttctt caatttcgaa ctcctcgacc agaaaaccgt
     7921 tgatttctcc gatgatttta ctcttctcca tgtcccgggt tcttgatact catttttttg
     7981 ggagagcaaa ggtaaggata ttttttttgt tgtggatgaa aaaagtcgtc agtcagcagt
     8041 cggcggtcgg cagccagcag tcggaaagca aataagagta ttcataaggt aggcatatgg
     8101 tgattgatct tgcaaattta tacttgaggc ttatctccgg aggagatcaa taattgtaga
     8161 aacgagagag ggaagaatcc acgattttcc cctcccgccg aaatcccggt taattgcttt
     8221 gaattttgca cgggagggga ttaaggggag ggcgagtaat aaaattgcat tcaaaattta
     8281 ataatagtga ttaagtttat tatatttgac aactctgtcc aaaaattctt gtctaaatga
     8341 atgaattaac atttggtgaa tacttaaggc ttcgtagaga agaactatca cttcctttga
     8401 ggaaagttgc ttcatatctt gatattgata cttcaacgtt gagtaaagtt gaaaggggag
     8461 aaaggccagc ttccaaagat tatttaaaac cccttgcaga aatacttaac ttggatttaa
     8521 aggatatcca gacaaaatat atcgctgata aaattactaa ggattttgag gggcttgaac
     8581 atgtccaatc gggactaaaa gccgcagaaa atatgctaaa ccaaaagaga aaataatgcc
     8641 agagatattt gataatattg aaaattattt ggttgatgga ttgagataca cgcttgatgt
     8701 ttcaaatcgt gcggattttt gcgttggata tttcaatttg cgtggatgga aacaagttgc
     8761 agaaaatatc gaaccgtgga atggcattga aggtaattgc tgcaggttat tagttggaat
     8821 gcaaaagcca cctttggaga taataaagga atacttttca acttcggatc agctcactat
     8881 tgacaatcaa aaagctgctg caataaagaa aaaacttgca caggaattta aggaacagat
     8941 aacagtaggt gttcctaccg atgaagatga gagaggctta aggcaattgt cctcacagat
     9001 taaagcaaag aaagtcattg tcaaattatt tttaagatat ccgttacatg caaagcttta
     9061 tttatgcttt agagatgaca aaatcaatcc aattatcggc tatctgggta gcagtaattt
     9121 aaccctttca ggtttatctt tacagggcga gttaaatata gatgtgttag atcgtgatgc
     9181 atcaggaaaa ttatcaaaat ggtttaatga ccggtgggag gataagtttt gtattgatat
     9241 ttcggaagaa cttactgaaa taattgataa tagctgggct gctgataaac ttattcatcc
     9301 gtaccatatt tatcttaaga ttgcttacca tcttgcaagg gaagcaaggg ctggccttgc
     9361 ggagtttaaa gtttccaaaa tatttgaaaa gcaactacta gattttcaaa tgaaagcagt
     9421 attggttgct gcacatcatt tgaataagag aggcggtgta attataggag acgtcgttgg
     9481 tttgggtaaa accatcacag cagctgcttt agccaaaatg tttgaagatg attatcttct
     9541 tgaaacgcta atcatttgtc cgaagaatct ggttaaaatg tgggaagatt acaggcacaa
     9601 ataccaactt agagcgaaga tcattccctc ttctcaggtt caaacaatcc tgccaaagga
     9661 aagacgatat agaattgtcg taattgatga aagtcataac cttagaaacc gggaaggtaa
     9721 acgatatcgt tctattcaag agtacattca tttaaatgac agtaaggtga tcatgttaac
     9781 tgctacacct tataacaaaa cctatcttga tctttcaaat caacttcgtt tatttattcc
     9841 ggaagagaaa gaccttggta ttagcccgga aaggtttaaa gtactcccca ttttctagac
     9901 cagggatctg tcattcatag ttgatttaa
//
LOCUS       DAOPNH010000110         9811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7521_length_9811_cov_5.010455, whole genome shotgun sequence.
ACCESSION   DAOPNH010000110 DAOPNH010000000
VERSION     DAOPNH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7521_length_9811_cov_5.010455"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKZ74_07215"
     CDS             complement(<1..785)
                     /locus_tag="PKZ74_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPM92587.1"
                     /translation="MKRLIQILSVITLSVLTIWGIKGIFSDNDKEDLVKEMGFKTEGD
                     ARVLPANYLPVAPFSGPDFTTAAEKTVHGVVHIKSEFSVRTGGYDDFFAPFRDFFGYP
                     YRGDNTYTGFGSGVIISNDGYIVTNNHVVEGAGRVNVTLNNKREYDAEIIGTDPSTDL
                     ALLKIDGSDIPFITIGNSDNTKIGEWVLAVGNPFNLTSTVTAGIISAKARNINILGTP
                     GAIETFIQTDAAVNMGNSGGALVNMNGELVGINAAIASNTGSYA"
     gene            950..1753
                     /gene="dapF"
                     /locus_tag="PKZ74_07220"
     CDS             950..1753
                     /gene="dapF"
                     /locus_tag="PKZ74_07220"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPM92588.1"
                     /translation="MKIPFYKFHGTGNDFILINNRENEFVAREDLISRMCHRRFGIGA
                     DGLILLEKAPGYDFGMHYYNSDGKESTMCGNGGRCIVALADFLSLTGNDCRFKAIDGP
                     HRGKVLHREGNNWQVSLQMADVAIIENMNDEFVLNTGSPHLVTFADNVSTVDVDGEGR
                     AIRNREVFKSDGINVNFAEIRDDGLFVRTYERGVEAETLSCGTGVTASAIAFAIKSGC
                     FNEKVKVSTPGGVLAVRFEYNKLGHFSSVLLEGPATLVFVGETDTNYYL"
     gene            1773..2318
                     /locus_tag="PKZ74_07225"
     CDS             1773..2318
                     /locus_tag="PKZ74_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM92589.1"
                     /translation="MKPISGGIVSLRALEPEDADLLYEWENDCRIWHLSNTIAPLSRF
                     ALEQYILNTGQDIFATRQLRLMIDLQSKEGKTTIGSVDLFEFEPLHLRAGAGILVNEK
                     YRGRGYASEALTLLIGYAFGTLRLHQLYANVSASNHDSIKLFEKNGFLPAGVKKDWNR
                     LPNKWEDELMFQLINPSRVIR"
     gene            2305..3393
                     /gene="mltG"
                     /locus_tag="PKZ74_07230"
     CDS             2305..3393
                     /gene="mltG"
                     /locus_tag="PKZ74_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPM92590.1"
                     /translation="MLSDKMAVQIRKPEPRKKRGKFHRIILLILAGAVVISLVAAWSL
                     FNMILKPNVTVPGGKASLYIPSGSTYQDVKGILKSNAWLRNSSSFEWMAQRKNYPARV
                     KPGHYIIHNGMSNNSLVNMLRSGVQTPVDVTFNNILNKEELAGKIAGQIEADSLSLIE
                     CWNDKEFLKSLNTNPAKVIMLFLPDTYELWWTTSAREFTQRMAKESDRFWNEERLKKG
                     RDARMSREEVIILASIIEKETQMNDEKPVMAGVYINRLKKGWPLQADPTVKFALGRLD
                     LKRVLKIHTEFDSPYNTYKYTGLPPGPICIPSKASIEAVLNFRKHDYMFFCAKADMSG
                     YHVFSRTLAEHNRYAAAYQAELNRRRIR"
     gene            3458..4363
                     /locus_tag="PKZ74_07235"
     CDS             3458..4363
                     /locus_tag="PKZ74_07235"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPM92591.1"
                     /translation="MLFATVTISQPIDYHDASISDSTNPAEPDKKRLTGVSATAGGLY
                     AGTMTGLYYLWYADYPLSKFHFINDNNHWLWMDKIGHVTTSYWIGKIGYESLRWSGLE
                     EKKAVWYGGTWGLVYLTTVEIFDGFSREWGASDGDFAANVMGTAAFIGQQLIFEDQIF
                     VFKYSFHPTKYADYRPDLLGENMAQSLLKDYNGQTYWLSANLSAFLPSESRFPRWLAF
                     SFGYSAEGMLGAKSNPDEYNGKPLPHFGRYNQYFITLDLDLTKIRTRSETLRFLLNLV
                     GYVKVPFPAVEFNKKDHVKFHWLYF"
     gene            4425..5588
                     /locus_tag="PKZ74_07240"
     CDS             4425..5588
                     /locus_tag="PKZ74_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM92592.1"
                     /translation="MSKILVVDDERSIRNTLRDILTYEKYDVSDAESGIVALEMLRQS
                     EFDVILLDIKMPQMDGMEVLDHILAEYEVPVIMISGHGTIETAVDAIKKGAYDYVAKP
                     LDLNRLLITIRNALDKSRLMTETRSLKKRVSKKYEIIGETEVMNGIREMIDRVAPTHA
                     RVLITGDNGTGKELVARQLHEKSLRARGPFIEVNCAAIPSELIESNLFGHEKGSFTSA
                     IKQRKGDFELATGGTIFLDEIGDMSLTAQAKVLRALQENKIMRVGGEKEIPVDVRVIA
                     ATNKNLKDEIAAGRFREDLYHRLSVILIHVPPLRERVDDIPVLAHYFMEEICAEQGKP
                     LMFFTDEAFEELKMMKWTGNIRELRNVVERLIILCDKEITDSDVRRFAQPLVI"
     gene            complement(5593..6756)
                     /locus_tag="PKZ74_07245"
     CDS             complement(5593..6756)
                     /locus_tag="PKZ74_07245"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPM92593.1"
                     /translation="MRAKKLLSRFLLSYCLMALTFCGSEMNAQVRVSKVSDPSQLTGK
                     KGIFYSLPRTKIHINLGIVRTEQIAGPLAEYAKEMLGIDKVVEKNMVTYAIDHVAIMT
                     ESETDPEQLFLVEKEEKAQGEVWIAFGKREPVVALEKFDKASHPDGFTPWNGQHIPLA
                     DPQSLFRKYTDSPVREVIDTIVRKVSIDTLVMVDMIFKRTLAEFSDLEKAQEAAARIR
                     QIEQDQYNLLIGYQETAYSIESLQFMYDKLEELRLEYLKLFTGLTLNTLMRYDLPVVP
                     SIGEDDQAYTLGGFSKSSGLVEAEGQNAIILKLISSESALSDLAKKQESFTGGIAYRL
                     PVKVKAAVFNQGKELVSKEIEVLQLGPVISLPSEFKRVEFDTETGNLRSVVIE"
     gene            complement(6774..8966)
                     /locus_tag="PKZ74_07250"
     CDS             complement(6774..8966)
                     /locus_tag="PKZ74_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPM92594.1"
                     /translation="MSNFRFKAIEIALSRSLDKKSPANGRKISEYFGEMTFGISMMRE
                     HLSKEAFTAVMSAIKKGEKIDRRVADQVAASMKSWALSKGATHYTHWFHPLTGATAEK
                     HDAFIVPVDDGKAIENFNGDQLVQAEPDASSFPSGGIRNTFEARGYTAWDPTSPAFIL
                     DSTLCIPTIFVSYTGEALDYKTPMLKALHALDKAAVEVCRYFDKNVTKVIATLGWEQE
                     YFLIDSALFAARPDLVLTGRTLFGHPSAKDQQLSDHYFGSIHERVAHFMRDVEYESHR
                     LGIPLKTRHNEVAPNQYECAPIFEEVNLAVDHNQLLMHLLKKVANHHNFTVLLHEKPF
                     ASVNGSGKHNNWSMATDTGVNLLSPGKTAKTNLQFLVFIVNVLKAVHEHAGLLRAGIS
                     SAENDNRLGGHEAPPAIISAFIGSHLTKILDEIEQKVRKGRISSDLQEELSVNISKIP
                     EILRDNTDRNRTSPFAFTGNKFEFRAVGSSDTCARAMIILNTIMADQLVSFKNDVDKL
                     LEEGVKKDEAIFKVLKRYITESKAIRFEGNSYSEEWVQEAGRRGLITARSTPEAFKEY
                     ISPKTVKLFNDHKVFTERELLARYDIKLESFTRKLQIESRMMGDLAKNHLIPTAIRYQ
                     NMLIENAMGLKQVLDAKTYVKLSKNQVDTIKDISDHVSQVKINVDLMLEERKKANRAG
                     SLEEMTCAYDEKVKPYFDIIRYHIDKLELLVEDNLWPLPKYREIMFLK"
     gene            complement(8970..>9811)
                     /locus_tag="PKZ74_07255"
     CDS             complement(8970..>9811)
                     /locus_tag="PKZ74_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPM92595.1"
                     /translation="MFNTVWESDYSRMIFGDWHDKRFYLKQILSGAFIAIVMTGLDQE
                     MMQKNLSCRTLRDAQKNMFSFSMVLVLVNFLFLFLGAALYLFAHDKGIDLPHRSDDLF
                     PVIAINYLGPVAGIVFIIGLISAAYPSADGALTSLTTSFSIDFLGLNKKDHLSEKQKV
                     KARYLVHTGFALLLLVVIVIFRAINDRAVIDKLFTIAGYTYGPLLGLYAFGLFTKWKV
                     KDRWVPYIAVVSPVLCYLLSIYDQMLLNNYNFGFELLIINGIFTFLGLFLARKRDNPI
                     SSL"
BASE COUNT         2631 a         2201 c         2337 g         2642 t
ORIGIN      
        1 gcgtaggatc cggtattgct tgcaatggcc gcattgatcc cgaccagttc gccgttcata
       61 ttgaccaggg ctccgccgct attacccata ttaacggctg catctgtctg aataaatgtc
      121 tcgattgctc ccggtgtgcc cagaatgttg atattcctag ccttggcgct gataataccc
      181 gccgtaaccg tggatgtaag gttgaacggg tttcccacag ccagtaccca ctctcctatt
      241 ttcgtattgt cagaattacc gatggtaata aatggaatat cgctgccgtc aatcttgagg
      301 agtgcaaggt ccgtagaagg atctgtacct atgatttccg catcgtattc ccttttgttg
      361 ttcagcgtta cattcactct tcctgctcct tccaccacat ggttgttggt tacgatatag
      421 ccatcattgg aaattatgac acctgaaccg aatcctgtgt aggtattatc tccgcgatac
      481 gggtatccga aaaagtcgcg gaacggggca aaaaaatcgt cataacctcc cgtcctgaca
      541 ctgaactccg attttatatg aacgacgccg tgtacagttt tttcagcagc agtagtaaag
      601 tcaggccctg agaaaggtgc gacgggcagg taattagcag gtaacactct ggcatcacct
      661 tccgttttaa aacccatttc tttcaccaaa tcctctttat cattatccga aaagataccc
      721 tttattcccc aaatggtgag tacagagagg gtaataaccg ataatatctg aattaacctt
      781 ttcattgttc aattttttga tttgctatta taataatttc gggaaaaacg ccatgaaatt
      841 cgcaaatagt gtgcctttcc actgttaaat tatgttaaaa gaaaggcaaa tcaatattgc
      901 tctttaactc catttttctc tacttttgaa tcttaaattt agaaaaccat tgaaaattcc
      961 attttataag ttccacggaa caggcaatga tttcatcctg attaacaacc gggagaatga
     1021 atttgttgca agggaagatt taatatcccg gatgtgccac cggcgttttg gaatcggagc
     1081 cgatgggctt atactgctgg aaaaagctcc cggttatgat tttggcatgc attattacaa
     1141 cagcgatgga aaggaaagta cgatgtgcgg aaatggcggc cggtgcatag tggccctggc
     1201 tgactttttg tcactcaccg ggaatgattg ccggtttaaa gcgattgacg ggcctcaccg
     1261 cggtaaagtt ctgcaccgcg aaggaaataa ctggcaggtg agcttacaaa tggcagatgt
     1321 agccatcata gaaaacatga acgacgaatt cgttctcaat accggctctc cgcacctggt
     1381 gacatttgcc gacaatgtca gcaccgtgga tgttgacggt gaaggcaggg cgatcaggaa
     1441 ccgcgaagtt tttaagtccg atgggattaa tgtgaatttt gcagaaatcc gggatgacgg
     1501 attatttgtg cggacttatg agcggggtgt cgaagcggaa accctttcct gtggcaccgg
     1561 ggtcacggca tctgccattg cttttgccat aaaaagcggg tgctttaacg aaaaggtcaa
     1621 agtttcgact cccggaggtg tactggctgt caggtttgaa tataataagc tgggccattt
     1681 ttcgtctgtt ttactggaag ggccggcaac actggttttt gtgggagaaa cagacaccaa
     1741 ttattattta taagatgatg accgaatcac tcatgaaacc gatttccggc gggattgttt
     1801 ccctccgtgc gcttgagccg gaggatgccg atctccttta tgaatgggag aatgattgca
     1861 ggatatggca tctttccaac accattgccc ctttatcgcg atttgccctg gaacagtaca
     1921 ttctgaatac cggtcaggat atcttcgcta cacgtcagct ccgcctgatg attgacctgc
     1981 agagcaaaga gggaaaaaca actatcggat cggtcgactt gtttgaattt gagccgttgc
     2041 atctccgtgc aggtgcaggt attcttgtca atgaaaaata caggggcaga gggtatgctt
     2101 cagaagccct tactttgctg atcgggtatg ctttcgggac cttgcgcctg caccagctat
     2161 acgccaatgt ttcggcatcg aatcacgaca gcattaaact atttgagaaa aacgggttcc
     2221 ttcccgctgg tgttaaaaag gactggaaca ggcttccaaa taaatgggaa gatgaattga
     2281 tgttccagtt gatcaatcca tcacgtgtta tccgataaaa tggctgttca gatcagaaaa
     2341 ccggaacccc gaaaaaagag agggaagttt caccgaatta tcctgctgat ccttgcaggc
     2401 gcggtggtaa tctcccttgt tgcagcctgg tcgttgttta atatgatttt aaaaccgaat
     2461 gttacagtcc ccggcgggaa agcttcttta tacataccat ccggctcgac ctaccaggat
     2521 gtgaaaggaa tactgaaaag taatgcgtgg ttgagaaaca gctcttcgtt cgaatggatg
     2581 gctcagcgaa aaaactaccc ggctcgtgtc aagccaggtc attacatcat ccataacggt
     2641 atgagcaaca atagcctggt gaatatgctc agatccggag tgcagacgcc tgtcgatgtt
     2701 acattcaaca atatcctgaa taaggaggaa ctggccggaa aaattgccgg tcaaattgaa
     2761 gcagattcat tgtcactgat cgaatgctgg aatgacaagg aatttttgaa atcgctgaat
     2821 acgaatcccg caaaggtgat tatgcttttt ctgcctgaca cctacgagtt gtggtggacg
     2881 accagcgccc gggaattcac gcagaggatg gcaaaagagt cggaccggtt ctggaatgag
     2941 gaacggttga agaaaggcag ggatgcccgt atgagccggg aagaggtgat catccttgcc
     3001 tccatcatcg aaaaagagac ccagatgaac gatgaaaaac ctgttatggc tggtgtttac
     3061 atcaaccggc tgaaaaaagg ctggccgctg caagctgatc cgacagttaa attcgcactg
     3121 ggccggcttg acctgaaaag ggtactgaaa attcatactg aattcgattc cccttacaat
     3181 acgtataaat ataccggatt accgcccggg ccgatctgta taccctcaaa agcttctatc
     3241 gaagcggtat tgaatttcag gaaacatgat tacatgtttt tttgtgcaaa agcggacatg
     3301 tcgggatatc atgtcttctc caggaccctg gccgagcata atcgatatgc cgcggcttat
     3361 caggcagaat tgaacaggag gagaatcaga tgaaatgttg attgttctaa aaaataaggt
     3421 gctaaagggc aaaataatat tgcatggatt gtttttcatg ctgtttgcca cagtgacaat
     3481 ttcccaaccg attgattatc atgacgccag tatttcagat tccacgaatc cggctgaacc
     3541 tgataaaaaa agactgaccg gcgtttcagc cactgccgga gggctatacg caggtaccat
     3601 gaccggatta tattatttat ggtatgccga ttacccgctc tctaaatttc attttattaa
     3661 cgacaataac cattggttgt ggatggataa gatcgggcat gtcacaactt cttactggat
     3721 tggaaagatt ggatatgaaa gtcttagatg gagcggattg gaggaaaaga aagcggtatg
     3781 gtacgggggg acatggggat tggtatacct gactactgtc gaaatcttcg atgggttttc
     3841 acgggaatgg ggcgcctccg atggtgattt tgcagcgaat gtgatgggta ctgctgcatt
     3901 tatcggtcag caattaatat ttgaagatca gatttttgtg tttaaatact catttcatcc
     3961 cacaaaatat gcagactatc ggcctgatct gctcggggag aatatggcgc agagcctttt
     4021 aaaggattat aacggacaga cctactggtt atcagccaat ttgtctgctt tcctcccgtc
     4081 agagagccgg tttccccgat ggttggcgtt ctctttcggt tacagcgcag aagggatgct
     4141 cggggccaaa tccaatccgg atgaatacaa cgggaaaccc ctaccccatt tcggcaggta
     4201 taaccagtat tttataacac ttgacttgga cctgacaaag atcaggacac gttccgaaac
     4261 attacgattt ttactcaacc tggtcgggta tgtgaaagtt ccgtttcctg ctgttgaatt
     4321 caataagaaa gaccacgtaa agtttcactg gttgtatttt taagtacctg aagggtgcga
     4381 ccatcatcag ttttgcctaa ttttaccgga tcaatcatta tattatgtcg aaaatactgg
     4441 tagtcgatga tgagaggagt atccgtaata ccttgcggga tatcctgaca tatgaaaagt
     4501 atgatgtgag cgatgccgag tcgggtatag tggccctgga gatgctcagg caatcagaat
     4561 ttgatgttat tctcctggat atcaagatgc cgcaaatgga cggtatggaa gttctcgacc
     4621 atatactggc cgaatatgag gttccggtga tcatgatttc gggtcatgga acgattgaaa
     4681 cagccgtcga tgccatcaag aaaggggcct atgattatgt tgccaaacca ctggatttaa
     4741 accgtttgct gattacaatc cggaatgcgc tggataaatc gaggttgatg accgaaaccc
     4801 ggtcattgaa aaagagagtc agcaaaaagt acgagatcat cggtgagacg gaggtgatga
     4861 acggaatccg cgagatgatc gaccgggtgg caccaaccca tgcccgggta ctgataaccg
     4921 gtgacaacgg tacagggaag gagttggttg ccaggcaact ccatgagaaa agcctgcggg
     4981 cacgaggacc attcattgaa gtcaactgtg cggcaatccc ctccgaactg atagaaagta
     5041 atctattcgg acatgaaaaa ggttcattta cttcggcgat caagcagcgg aaaggggatt
     5101 ttgaactggc cacgggcgga acgatttttc tggacgagat aggcgatatg agccttacgg
     5161 cccaggcaaa agtattgagg gcgctgcagg aaaacaagat catgcgtgtt ggtggcgaaa
     5221 aggagatacc ggtggatgtc agggtgattg cggctaccaa taaaaacctg aaagatgaaa
     5281 ttgcggccgg aaggtttcgt gaagatctgt accaccggct gagcgtgatc ctgatccatg
     5341 tcccgccgct cagggaaaga gtggatgata ttcccgtcct ggcgcattat tttatggaag
     5401 agatatgcgc agaacagggc aaacccctga tgttttttac agacgaagct tttgaagagt
     5461 tgaagatgat gaagtggacc gggaacatca gggaactccg gaatgtggta gaaagattga
     5521 tcatcctttg cgacaaggaa attaccgatt ctgacgtcag gcggtttgcc caaccgctgg
     5581 tcatctgaag atctattcga taaccactga tcttaaattt ccggtttcag tgtcaaattc
     5641 cacccgtttg aattcggagg gcaggctgat gaccgggccc agttgcaata cttcgatttc
     5701 ttttgagacc agctctttac cctggttaaa aacagcagct ttgactttaa caggcaaacg
     5761 gtaagcaatg cctcccgtaa aagattcctg cttcttcgcc aggtcggaca gagcggattc
     5821 acttgatatc agttttaaaa tgatcgcatt ctgtccttcc gcttcgacca ggcctgagct
     5881 tttagaaaag ccgcccagtg tataggcctg gtcatcttca ccgatagatg ggactacagg
     5941 aagatcgtat ctcatcaggg tattcagggt caggcccgta aatagcttaa ggtattccaa
     6001 gcgcagttct tccagtttat catacataaa ttgcaaagac tcgatggaat aggccgtttc
     6061 ctggtacccg atcagcaggt tgtattggtc ctgttcgatc tgccgaatcc tggcagccgc
     6121 ttcctgcgcc ttttccaggt cggagaactc tgccagggtg cgcttgaaaa tcatatccac
     6181 catgaccaaa gtatcgatac ttacctttcg cactatggta tcgatcacct ctctgacagg
     6241 cgaatcggta tatttcctga aaagtgactg tgggtcggca agcggaatat gttgaccgtt
     6301 ccatggtgta aatccatccg gatgagaggc cttatcaaat ttttccaacg cgacgaccgg
     6361 ctcccttttt ccgaatgcta tccagacttc cccctgagct ttctcttcct tttcgaccag
     6421 gaaaagctgc tccggatccg tttccgactc ggtcattatg gcaacatgat caatagcata
     6481 agttaccatg ttcttttcca ccactttgtc gatacccagc atctcttttg catattctgc
     6541 cagtgggccg gctatctgct cggttctgac gatgcccagg ttaatgtgga tcttcgtcct
     6601 gggcagggaa taaaaaatgc cctttttacc tgtcaactga gaaggatcag aaacctttga
     6661 aaccctaacc tgggcgttca tttcgcttcc gcaaaatgtc aaagccatca ggcaatacga
     6721 taaaagaaat cgggataaca actttttggc tctcataatc aagcaattga aatctatttc
     6781 aggaacatga tctcccggta tttgggcaaa ggccacaaat tatcttcaac cagcagttcc
     6841 agtttatcaa tatgataacg tatgatatca aaataaggtt tcaccttttc gtcgtatgcg
     6901 caggtcattt cttccaatga accggcccgg tttgcttttt ttctttcctc cagcatcagg
     6961 tcaacattta ttttcacctg actgacatga tcggaaatgt ctttaatagt atccacctgg
     7021 tttttagata acttaacata ggtttttgcg tccagaacct gtttaagccc catggcattt
     7081 tcaatcagca tgttctgata gcggatagcg gtaggaatca ggtggttctt ggccaggtcg
     7141 cccatcatcc tggattcaat ctgcaacttc cgggtgaaag attccagctt gatatcgtac
     7201 cgtgccaata actccctttc agtaaagact ttatggtcat taaacaattt aactgtctta
     7261 ggtgaaatgt attctttaaa agcttccggg gtggaacgcg ccgttataag gcctctcctt
     7321 ccggcttcct gcacccattc ttcgctatag ctgttccctt caaaacggat agctttggat
     7381 tctgttatat atcttttcag taccttaaag atcgcttcat ctttctttac cccttcttcg
     7441 agcagtttat cgacatcatt tttaaagcta acaagctggt cggccatgat agtattgagg
     7501 atgatcatag ccctggcgca ggtatctgac gagcccacgg ccctgaactc aaatttattg
     7561 cccgtaaagg caaacggaga ggtacggttg cggtcggtat tgtcacggag gatctccgga
     7621 atctttgaaa tattgacgct gagttcttcc tgtagatcac tggaaattcg acctttcctg
     7681 actttttgtt caatttcgtc aaggatcttt gtcaggtgag aaccgataaa tgctgaaatg
     7741 atggccgggg gcgcctcatg accaccgagc ctgttatcgt tttcagccga cgaaatgccg
     7801 gctctcagca gccctgcgtg ttcatggacg gctttcagaa cattaacgat aaaaacgagg
     7861 aactggagat tggttttggc cgtttttccc ggcgaaagca ggttaactcc ggtatcggtt
     7921 gccatcgacc agttgttatg cttaccagat ccgttcacgc tggcaaacgg tttttcatgc
     7981 aacaacacgg taaagttgtg atgattggct acttttttca ggaggtgcat caggagttga
     8041 ttatgatcca ccgccaggtt gacctcctcg aaaatcggag cacattcgta ctgatttggt
     8101 gcaacctcat tgtgcctggt tttcaagggt atgccaagcc tgtgtgattc atattccaca
     8161 tctctcatga aatgggctac cctttcgtgg atacttccga aataatgatc cgataattgc
     8221 tggtccttgg cagaagggtg gccaaagagc gttcttccgg tcaggaccag gtcgggccgg
     8281 gcggcaaata acgccgaatc gatcaggaaa tattcctgct cccatccgag cgttgcaatg
     8341 accttggtca catttttgtc gaaataccgg caaacttcca cagcggcttt atccagtgca
     8401 tgaagagctt tcagcatagg cgtcttgtag tcgagggctt ctccggtata agaaacgaat
     8461 atggtaggga tacagagcgt tgaatccagg ataaaagccg gtgaggttgg atcccaggct
     8521 gtgtaaccgc gagcctcaaa ggtattccgg atgcctccgc tgggaaagct agacgcatcg
     8581 ggttcagcct gcacaagctg atcgccgtta aaattttcga tcgcctttcc gtcatcgacc
     8641 ggtacaatga acgcatcatg tttttcagca gtagcgccag tgagtggatg gaaccaatgc
     8701 gtataatgtg tggccccttt gcttaacgcc catgatttca tcgaagcggc aacctggtcg
     8761 gctactcgcc ggtcgatctt ctcccctttt tttatggcgc tcatgaccgc tgtaaatgcc
     8821 tccttggaaa gatgttcgcg catcatgctg ataccgaagg tcatttcacc aaaatactct
     8881 gaaatctttc ttccgtttgc cggggatttt ttatccagag acctggataa agctatttca
     8941 attgctttaa atctgaaatt actcatacac tataacgaac taattgggtt atctcttttc
     9001 ctggccagga ataagcctag aaatgtaaaa ataccgttaa tgatcaaaag ctcaaatcca
     9061 aagttataat tatttaataa catctgatcg taaatgctta ataagtaaca taatacgggc
     9121 gaaacaacgg ctatatacgg gacccatcga tccttgactt tccattttgt aaacagcccg
     9181 aaagcataaa gacccagcaa gggcccgtac gtataaccgg caatagtgaa gagtttgtcg
     9241 atgactgccc ggtcgttgat agccctgaaa atcacaatga ccaccagcag cagcaacgcg
     9301 aacccggtat ggaccaggta cctggctttt actttttgtt tttctgataa gtgatccttt
     9361 ttattcaagc ccaggaaatc aatgctgaac gatgtggtga gcgacgtgag tgcaccgtcg
     9421 gcactgggat atgcggccga aatgagcccg atgatgaata cgataccggc aactggtccg
     9481 agataattga tagcgataac ggggaaaaga tcgtcggacc gatgtggcag gtcaatgcct
     9541 ttgtcgtgag caaagagata aagggcggct ccgagaaaaa ggaaaagaaa gttgaccagc
     9601 accaggacca tgctaaagga gaacatgttc ttctgggcat ccctcagagt cctgcaactc
     9661 agattcttct gcatcatttc ctggtcaagc cccgtcatga caatagcaat aaaagctccg
     9721 ctaaggatct gtttcaggta aaagcgttta tcgtgccagt caccgaatat catccggcta
     9781 taatcactct cccagacagt attgaacatt t
//
LOCUS       DAOPNH010000111         9801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7535_length_9801_cov_7.847014, whole genome shotgun sequence.
ACCESSION   DAOPNH010000111 DAOPNH010000000
VERSION     DAOPNH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7535_length_9801_cov_7.847014"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="PKZ74_07260"
     CDS             complement(<1..1965)
                     /locus_tag="PKZ74_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92596.1"
                     /translation="MISSLLNSIEKLKVQILLTTGLLIGVLATGQSSTLTILTPNGGE
                     SYVSGQTIGVTWENTGDPEDLMIEVTEDGGAYWYYMAYVNAGDTSTTLYFQYYGNPTD
                     LAKVRISSYANPSNSDESDSYFTILESPVYFNSPYFGDRYYVNHPVNISWYSYTLTSF
                     NLDFSYDNGATWNSLAANYTGFDFEWDSPSQITDQALIRISDPADPASYGLSPVFSII
                     DEPVAVITQPNGGEIWNYGEYQTVSWDGSNLPPYVNIDFSYDGGLNWTSLGFGYSEPD
                     SGSAQVSVPYYSTENAKVRVTDYSYGFTLDESDATFSVVVPPVIVYYPSGGEYYNGQT
                     TYLNWLALQEISLLNFELSTDNGQTWTLIAEDIPANQGYYYWTVNGTPSQDCLIRMSN
                     STDPSQFGLSGQFTILETPVLTLQEPSAGEIWNTGTPYTISWTYENPAANYIYLEYSV
                     DNGSTWSYIGYVTIDGPQGSFNWTTPDVSSDQCRIRIMDYYLNFVADTSEIFTIITFP
                     ETPICMVSVDSTTNHNVIVWEKPASELIDRFVVYKETSEANVYEIIGTLDYNEQAVLT
                     DTNSNPNVKAYRYRLGFMDAQGHYFPAGADHQTIHLTINQGVGNTWNLIWTGYVGFEA
                     SSYNIHRKTGSGNYEPIGSTSASFTSYTDLT"
     gene            2180..3469
                     /locus_tag="PKZ74_07265"
     CDS             2180..3469
                     /locus_tag="PKZ74_07265"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HPM92597.1"
                     /translation="MTEFQPTRYHLRSLITGNSFPDVNWMLDEPGEGKPSLLQTEYEQ
                     AQLELLGDENGFYKFSGWLPVKKVLQGSSAPVTYKSKDLAVHLGLENLFITFSGWWPE
                     KGAGMRTCSFKETEAYSVCARLDEKNDKVLVVASAGNTARSFARVCSDNNIPLLLCIP
                     EDNLDALWFDTPVNDCVKVICTRSGSDYFDAIHLSALACKLDDFLPEGGAKNVARRDG
                     MATTVLSAVTTIGQIPGYYFQAVGSGTGAIAAWEANKRFQADGRFGKNKMKLIVSQNA
                     PFLPMHDAWKAGSRELLPLDDELARRQVEEIDAKVLSNRKPPYSIHGGLFDALKDTDG
                     DILPVTNEEAKKAGLLFLQKEGVDLHPAAAVATASLIKACQDQTIDKKAVIMLNITGG
                     GEELFRKGRNLFYLKPNALFDINPPIEEVAKELERLF"
     gene            complement(3535..4152)
                     /locus_tag="PKZ74_07270"
     CDS             complement(3535..4152)
                     /locus_tag="PKZ74_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPM92598.1"
                     /translation="MKKTFLFLLLFSFIATTGFAQLELKPAVGLNVARFDSDPVFSNG
                     ADSMLTNPGAGFQLGASLAIGRKLYVEPGVFYSKMVQGFETSDIEKEDFTYSANYIRV
                     PLNFGFKFIGTQTSLAGLKIFLGPSMFIPLSIQENTYPIAKTDVKSPLFDFNVGAGLT
                     IWNVFLDVSYGWGLSPQFKDDPIEAKMQAFYANVGFRFKLINDEE"
     gene            4313..5536
                     /locus_tag="PKZ74_07275"
     CDS             4313..5536
                     /locus_tag="PKZ74_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPM92599.1"
                     /translation="MMKIKVILLILVSAITLSSCSKDPDGPKQPKPINLPQKGQQVIS
                     ASNRFGIDLFRVTAGEENGNMMLSPLSASTALTMLLNGCQSETYNQIRDMLGYGELTT
                     QEINETYNSLVGQLLAVDPEVKLSLANAVWYKQGFNVKQDFLSVMDSSFQAAIGALDF
                     SQPSALETINNWAKDNTNGKIPKVLDEIDPDMVMFLMNALYFKGNWTYQFKESQTSDL
                     PFTLKDGSITDVKMMRQMLPARYFYGNNALAVELPYGQQNFAMVIILPNENLDAYIVN
                     FGEAGWQEIVEGLDSYAEPDDIEMILPKFKFEYEKILNDQLKALGMTDAFDPFLADLS
                     GIADADLVVDFVKQNTFVDVNEEGTEAAAVTTIGIKEVSMPPTIMIDKPFIFAIRERM
                     TNSLLFLGKVEHPGY"
     gene            5647..6177
                     /locus_tag="PKZ74_07280"
     CDS             5647..6177
                     /locus_tag="PKZ74_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HPM92600.1"
                     /translation="MKIQKLLAVAVLVLFAAALLPAQDYNYIGAVKCKMCHNSEKAGF
                     AYNIWKESLHAKALESLGSAKSLEYAKKNGIADPTKDPKCLDCHSTYHMVPKDNIETL
                     LPTEGVSCESCHGPGSAYKTNTIMKSRELSMKNGLILPTKEVCEKCHNKNNPFYKPFN
                     YEEAVKKIAHPTPKAQ"
     assembly_gap    6418..6517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6573..7100)
                     /locus_tag="PKZ74_07285"
     CDS             complement(6573..7100)
                     /locus_tag="PKZ74_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPM92601.1"
                     /translation="MKKRSMAELERISAEQFKMAEKMPVVIVLDDVRSAYNVGSIFRT
                     ADAFRIEGIRICGISARPPHKDISKTALGATESVDWKYYSVVEEAVAGLRNAGFRIYA
                     VEQADESVFLQDFTPARDEKIAIVFGHEVFGVKEEVLKMADGCIEIPQFGTKHSFNVA
                     ISAGIVLWDISLKIR"
     gene            7179..9782
                     /gene="mutS"
                     /locus_tag="PKZ74_07290"
     CDS             7179..9782
                     /gene="mutS"
                     /locus_tag="PKZ74_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPM92602.1"
                     /translation="MIDTPLMRQYASIKAKYPDALLLFRVGDFYETFGEDAVKTSKIL
                     GIVLTRRANGAATFVELAGFPYHALDTYLPKLVRAGQRVAICDQLEDPKLAKKIVKRG
                     VTELVTPGVSYNDKILEHKENNFLCSLHLGEKTSGAAFLDISTGEFYAAQGNNDYIDK
                     LLQGFKPSEVVIQKTKRERFQQLFGNKFYTYTFDDWVFTEEFAGDILTRHFGTTSLKG
                     FGVEAMPIAIIAAGGAFHYLAETHHDKIQHIVKISRIEEDKYVWLDRFTVRNLELVSS
                     INEKGRTLLDVIDRTITPMGSRMMRRWIVLPLKEKKPIEERLETVEYLINHEELAENL
                     ARQLKITGDLERIISKVSVGRVTPREVVQFRRALYAAEEIKNLAGTTGHPVLQKISEQ
                     LNPCKIIRDRIENEIQDDPPATVAKGGVMKTAVSPELDELRDLLFSGKDYLQKLQQRE
                     IANTGITSLKVGYNNIFGYYLEVTNAHKEKVPAEWNRKQTLVGSERYITEELKEYEQK
                     ILGAEEKILELESRLFDELIQGIRDYIRPVQLNANWIAHLDCLLSFAIIAKENKYTKP
                     GINEGFAIEIREGRHPVIELELPLGEKYVTNDISLDHEKQQIIIITGPNMSGKSALLR
                     QTALIVLMAQLGSFVPAEEANIGMVDKIFTRVGASDNISSGESTFMVEMNETASILNN
                     ISTRSLILLDEIGRGTSTYDGISIAWAITEFLHEHPVYRPKVLFATHYHELNEMAATF
                     KRIRNFHISVKEIGNQVIFLRKLRKGGSEHSFGIHVARLAGMPPKVIERANELLKVLE
                     RSHSNDELAKMAKEIGASDKDQFQLSFIQLDDPLLEQIKQDILHTDIDNLTPVEALMK
                     LHTIRKRLSGG"
BASE COUNT         2674 a         2283 c         2384 g         2360 t
ORIGIN      
        1 ggtcaggtcg gtgtagctgg taaagctggc cgatgtgctt ccgatcggtt cataattgcc
       61 cgaaccggtt ttccggtgga tattgtaaga tgatgcctca aatcccacat agccggtcca
      121 gatcaggttc caggtattgc caactccctg gttgatcgtc agatggatag tctggtgatc
      181 ggcgccggcc gggaaataat gaccctgggc atccatgaat cccagtcggt aacgataagc
      241 tttcacattc ggattgctgt tggtgtcggt aagaacggct tgttcattgt aatccagggt
      301 gccgataatt tcatatacat tcgcttccga tgtttccttg tataccacga accggtcgat
      361 cagttccgag gcaggctttt cccagacaat gacattgtgg ttagtggtgc tgtcgaccga
      421 aaccatacag attggggttt ccgggaaagt gatgatggta aagatttcgc tggtatcggc
      481 cacaaaattc aggtagtagt ccatgatcct gatccggcac tggtcagacg acacatccgg
      541 agttgtccag ttaaagctgc cttgcggtcc gtcgatagtg acataaccga tgtaactcca
      601 ggttgatccg ttatcgactg agtattccag gtagatgtaa ttcgcggcag gattctcata
      661 ggtccatgaa atagtgtagg gggttccggt attccagatc tcgccggcag acggttcctg
      721 tagcgtgagt accggggttt ccaggatcgt aaattgcccg ctgagaccaa attgggaagg
      781 atcggtggag ttgctcatcc ggatgaggca gtcctgggat ggcgtcccgt tgacggtcca
      841 gtaataataa ccctggttgg cggggatatc ttctgcaatg agtgtccagg tctgtccatt
      901 atctgtcgat aattcgaaat tcaacaagct gatctcctgc aaggccagcc agttgagata
      961 ggtcgtctgg ccgttatagt attcaccgcc ggaagggtag taaacgatga cgggaggaac
     1021 aaccacagaa aatgtcgcat cgctctcatc cagggtaaac ccatatgaat aatcggtgac
     1081 ccggactttg gcattctcag tggaataata tgggacgctg acttgtgccg atccgctatc
     1141 cggttcacta tagccgaagc ctaagctggt ccagttcaag ccgccatcat acgaaaaatc
     1201 gatattaacg taaggaggga gattgcttcc gtcccaggaa accgtctggt attcaccgta
     1261 gttccatatt tctcccccgt taggttgagt gataaccgcc accggttcat ctataataga
     1321 aaaaacaggg ctcaatccgt aactggccgg atcggccgga tcagaaatgc ggatcagtgc
     1381 ctgatccgtt atttgtgacg gcgagtccca ttcgaaatca aatccggtat aattagccgc
     1441 caggctgttc caggtggccc cgttatcgta tgaaaaatcc aggttgaatg aggtcagcgt
     1501 gtaggaatac caggaaatat ttacagggtg gttcacataa tacctgtcgc cgaaataagg
     1561 tgaattaaaa tataccggtg attccagaat ggtaaaatag ctatcgctct catccgaatt
     1621 ggaaggattg gcatagctgg agattcttac cttggccaaa tcggttggat tgccataata
     1681 ctggaaataa agggtggtag aagtatcgcc tgcatttaca taagccatat aataccaata
     1741 cgcccctccg tcttcggtaa cttctatcat caggtcttcc ggatcgcctg tgttttccca
     1801 ggtcacaccg atagtctggc cggatacata ggattctcct ccgttgggcg tgaggatagt
     1861 cagggttgag ctttgaccgg ttgctaatac accgatcagc aagccggtgg tcagaaggat
     1921 ctgcaccttc agtttttcga ttgagtttag tagagaagag atcatgatga gtaattttta
     1981 attcttaatt tttagttttt tattcttaat tatttcttta ttgtaaatga tttgatttta
     2041 gttgatggag caaaataaca gtatgaatac gggtattgaa aatttaaaac gatcagccgg
     2101 gaaaactgtt cactgaacgg gaatttttca gccccgggtg aatttcatac ctttgcccgg
     2161 aattaatgat gatgacccga tgactgaatt tcaacccacc cgataccatc tccggtcgct
     2221 tattacgggc aattcattcc ccgatgtgaa ctggatgctg gacgaacccg gtgaaggaaa
     2281 accttcgctg ctgcaaaccg aatatgaaca ggctcagctg gaattgctgg gtgatgaaaa
     2341 tgggttctac aagttttccg ggtggctgcc ggtcaaaaag gttctgcagg gttcctctgc
     2401 ccctgtcact tataaaagca aagacctggc tgtgcacctc gggctggaaa acctttttat
     2461 cacattcagc ggctggtggc cggagaaggg tgccggcatg cgtacctgtt catttaagga
     2521 aacagaggcc tattctgttt gtgcaaggct ggatgaaaag aatgataaag tacttgtggt
     2581 tgcatccgcc ggcaatacgg cccggtcgtt cgcccgcgtc tgctccgaca acaatatccc
     2641 tctcctgctc tgcatccccg aggataacct ggatgccctc tggtttgaca cacccgtaaa
     2701 tgactgtgta aaagtgatct gcacccgttc gggcagcgat tacttcgatg ccatccacct
     2761 gtcggccctg gcttgtaagc tggatgattt cttaccggaa gggggagcaa aaaatgtggc
     2821 acgaagggat ggaatggcaa cgacagtcct ttcggccgta acgacgatcg ggcagatacc
     2881 cggctactat ttccaggctg tgggcagcgg caccggggct attgcagcct gggaggctaa
     2941 taaacgcttc caggccgatg gccgtttcgg caaaaacaag atgaaactga tcgtatccca
     3001 gaatgcgcct ttcctgccga tgcacgatgc ctggaaagcc ggctcgagag aactgctccc
     3061 gctggatgac gaactggccc gccggcaggt ggaagaaatc gatgcaaaag tgttgtcgaa
     3121 ccgcaagccg ccgtattcta tccacggcgg attgttcgat gccctcaagg atacggatgg
     3181 cgatatcttg cctgtgacca atgaagaagc taaaaaagcc ggcctattgt tcctgcagaa
     3241 agaaggggtc gatctccatc cggctgccgc tgtggcaaca gcatcgctca tcaaagcctg
     3301 ccaggatcag accatcgata aaaaagccgt cattatgttg aatatcaccg gtggtggaga
     3361 agaattgttc agaaaaggta gaaatttatt ttacctgaag cccaatgccc tgttcgacat
     3421 caaccctcca attgaagaag tcgcaaaaga attggaaagg ttgttctgaa attgaatact
     3481 aaagacattg aaattcgaac tttgaacttt gaactttgaa ctttgaacta aattctattc
     3541 ttcgtcatta atcaatttga aacggaaacc cacattggca tagaaagcct gcatcttggc
     3601 ttcgatcggg tcatccttga attgagggct cagtccccat ccgtagctca catccaggaa
     3661 aacattccag atggtcagac ctgcgccgac attaaaatca aacaaagggc tcttaacatc
     3721 cgttttggcg atcgggtaag tattttcctg gatactcaga ggaataaaca tagagggacc
     3781 aaggaaaatt ttcaatccgg ccaggctggt ttgggtgccg atgaatttaa aaccgaaatt
     3841 cagcgggacg cgaatataat tggcactata ggtaaaatcc tctttctcaa tatctgaggt
     3901 ttcgaagccc tggaccattt tagaataaaa caccccgggt tcgacgtaca gtttccgtcc
     3961 gatcgccagg ctggcgccga gctggaaacc ggcaccggga ttcgtaagca tactgtcggc
     4021 gccgttgctg aagaccggat cgctgtcgaa acgggcgacg tttaaaccca ctgcgggttt
     4081 tagttctagt tgcgcgaaac cggttgtggc aataaagctg aatagtagta agaagagaaa
     4141 tgtctttttc atctttcaga ttttttacaa agattataat tttttaaacc ggaattgaaa
     4201 tttctttatc ggtcactgac tgaaaaaggg tgtcaattat cgcatagaat atatatcggt
     4261 ttggcaaggt ctttgcatct ttttcccttt ctgttatttg ctaaacacaa agatgatgaa
     4321 aataaaggtg attcttttga ttcttgtcag tgctattaca ttgtcttcct gctcgaaaga
     4381 cccggatggc cccaaacaac cgaaacctat caatctgccg caaaaagggc aacaggtcat
     4441 atcggccagc aaccgtttcg ggatcgacct tttcagggtt acagccggcg aagaaaacgg
     4501 caatatgatg ctttcgccgc tgagcgccag caccgcgctg acgatgcttc ttaacggatg
     4561 tcagagtgag acctacaacc agatcaggga catgctgggc tatggagaac ttaccaccca
     4621 ggagatcaat gaaacctata acagcctggt tgggcaactg ctggctgtgg atccggaagt
     4681 aaagctctcg ctggccaatg ccgtctggta caagcaggga tttaacgtta aacaggattt
     4741 cttatcggta atggacagct catttcaggc tgccatcggc gcattggatt ttagtcagcc
     4801 ttctgccctc gaaaccatca acaactgggc aaaagataac accaacggaa aaattccgaa
     4861 agtcctggat gaaatcgacc ccgatatggt gatgttcctg atgaacgcgc tttatttcaa
     4921 aggcaactgg acttatcagt tcaaggaaag ccagacttcc gatctgcctt tcacgctgaa
     4981 agacggaagt atcaccgatg ttaagatgat gcggcaaatg ctgccggccc ggtatttcta
     5041 tggcaataat gcattagcag tggaattgcc ctacggtcag cagaatttcg ccatggtcat
     5101 cattcttcct aatgaaaacc ttgatgctta tatcgtgaat ttcggtgaag ccgggtggca
     5161 ggagattgtt gaaggactgg attcttatgc ggaaccagac gacattgaga tgatcctgcc
     5221 caaatttaaa ttcgaatacg agaaaatact taacgatcaa ctgaaagccc tgggtatgac
     5281 cgatgcattt gatcccttcc tggctgatct ttccggcatt gccgatgctg accttgttgt
     5341 ggattttgtg aagcagaaca ctttcgtgga tgttaatgaa gaagggacgg aagcagcggc
     5401 tgtgaccact atcgggatca aagaagtaag catgcccccg acgataatga tcgacaagcc
     5461 cttcatcttc gcgatccgag aaaggatgac gaattcactc ctgttccttg gtaaagttga
     5521 acatcccggg tattaaaaaa acaattaaat tcatatgtga ttttttaaat taattgtata
     5581 cttttgattt ttgttaaatt tatcacaaaa attatttaat aactaaccga acgaaaacgt
     5641 ataactatga aaattcaaaa attattagct gttgcagttc tggtcctctt tgctgccgca
     5701 ctgctccccg cgcaggatta caattacatc ggcgctgtga agtgtaaaat gtgtcataat
     5761 tcggaaaaag ccggttttgc ttacaacatc tggaaagagt cgctgcatgc caaagcgctc
     5821 gagtcgctgg gaagtgcaaa atcgctcgag tatgccaaga agaacggcat tgccgatcct
     5881 accaaggatc cgaaatgcct tgattgccat tccacgtatc atatggtccc gaaagacaat
     5941 attgaaacgt tgctcccgac agaaggcgta tcctgcgaaa gctgccacgg gccgggaagc
     6001 gcatacaaaa ccaacaccat tatgaaaagc cgcgagcttt cgatgaaaaa tggcctgatc
     6061 ctcccgacaa aagaggtttg cgaaaaatgc cacaacaaga acaatccctt ctacaaaccg
     6121 tttaactatg aagaagcggt gaagaagatc gctcacccga cgccgaaagc acagtaggtt
     6181 ataaagaaat acagataaaa aaccgacggg taaacccgtc ggtttttttt tgaaagactt
     6241 ccttaatgct gtctcaagag tctgatattt cgaattgatt gcctgaaaac agttcgtgct
     6301 ccaacgacta aatattacaa agatggaacc gacggcttta gtcgtcggaa gaaaacctat
     6361 caggaaccga cggctttagt cgtcggaaga aaacctatca ggaaccgacg gctttagnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg tcggaagaaa acctatcagg
     6541 aaccggcggc tttagtcgtc ggttcccagg gattacctga tctttagtga aatatcccaa
     6601 agcacaattc cggcgctgat ggccacattg aacgaatgct tggtgccgaa ctgaggaatc
     6661 tcaatgcatc catccgccat ctttaatact tcttccttca cgccgaatac ttcgtggcca
     6721 aatacgatcg caattttttc atccctggcg ggtgtgaaat cctgtaaaaa aacgctctca
     6781 tcagcctgtt cgacagcgta aatcctgaag cctgcgtttc gcaggccggc aacggcctcc
     6841 tccaccacgg aataatattt ccagtcgacc gattctgtgg cgcccaaagc tgtcttggaa
     6901 atatctttgt gtggcggccg ggccgaaata ccacagatcc ggatgccttc aatccgaaag
     6961 gcatcggctg tgcggaagat cgatccgaca ttataagcgc tcctgacatc atcaagcaca
     7021 atgaccaccg gcatcttctc cgccatcttg aactgctcgg ctgaaatccg ctccaattcc
     7081 gccatcgatc tctttttcat acgataattt ccttatcgct aaattaatcc tatttttgct
     7141 acaatactgg atatactacg ttgaataaag agccggatat gattgatacg ccattgatga
     7201 ggcagtatgc ttcgatcaag gcaaaatacc ccgatgcatt attgctgttc agggtggggg
     7261 atttttatga gacgttcgga gaagatgcgg tcaaaacttc gaaaatcctg ggtatcgttc
     7321 tgacccgtcg ggccaacggc gccgctactt tcgtagaact ggccggcttt ccttatcatg
     7381 cactcgacac ctacctcccg aaactggtca gggccggaca gcgggtagcc atctgcgatc
     7441 agcttgaaga tccgaaactg gccaagaaga tcgttaaacg aggcgttaca gaactggtta
     7501 cacccggcgt atcctacaac gataagatcc tggagcacaa ggagaacaac ttcctgtgca
     7561 gcctccacct gggagaaaaa acatcgggtg ccgctttcct ggatatctcg acaggcgagt
     7621 tctatgccgc acaaggcaat aacgattata tcgataaatt gcttcagggt tttaaaccca
     7681 gcgaagtagt catccagaaa acgaagcggg aacggttcca gcaactgttc gggaataaat
     7741 tctacaccta cacatttgac gactgggtgt ttaccgaaga atttgccggc gatatcctga
     7801 cccgccattt cggaacgacc agcctgaaag gatttggcgt ggaggctatg cccatcgcca
     7861 tcattgcagc cggcggagct tttcattacc tggcggaaac acatcacgat aaaattcagc
     7921 atatcgtaaa aatatccagg attgaagagg ataaatatgt ctggctcgac cggttcacgg
     7981 tgagaaatct ggaactggtc agctcgatca atgaaaaagg gagaactctt ttagatgtga
     8041 tcgaccgcac catcaccccg atgggatcac gaatgatgcg gcgctggatc gttctgccgc
     8101 ttaaggaaaa gaagccgata gaagagcgcc tggaaacggt ggaatatctt atcaaccatg
     8161 aggaactggc cgagaacctg gccaggcaac tgaaaataac cggtgacctg gaacgaatca
     8221 tctccaaagt atcggtgggg cgggtcacac ctcgcgaagt ggttcagttc agacgggcgc
     8281 tctatgctgc agaagagata aaaaacctgg ccggaacgac agggcatccg gttttgcaaa
     8341 agatcagcga acaactgaat ccttgcaaaa tcatccgcga ccggatcgaa aatgagattc
     8401 aggacgatcc tcctgcaacc gttgccaaag gcggtgtaat gaagacagcc gtctcgccgg
     8461 aactggatga attaagagat ctgcttttct ccggcaaaga ttatctgcaa aaactgcagc
     8521 agcgggagat cgccaatacc gggatcactt ctctgaaagt cggatataac aatatcttcg
     8581 ggtattacct ggaagtaacc aatgctcata aagagaaagt cccggccgaa tggaaccgga
     8641 agcagacact ggtgggatcg gaaagatata taacagagga actgaaagag tacgagcaga
     8701 agatcctggg tgcagaggag aaaatcctgg agctggagag ccgccttttt gatgaactca
     8761 tccaggggat cagggattat atccggccgg tacaactcaa tgctaactgg atcgcacacc
     8821 tcgactgcct gctttcgttt gctatcatcg cgaaagagaa caaatacacc aaacccggga
     8881 tcaacgaagg attcgccatc gagatccggg aagggcggca cccggtgatc gagctggaac
     8941 ttccgctcgg cgaaaaatat gtgaccaatg atatctcact ggaccatgaa aagcagcaga
     9001 tcatcattat cacggggcca aatatgtcgg gaaaatcggc gctgctgagg caaacggccc
     9061 tgatcgtcct gatggcccag ctcggttcat ttgtgccggc cgaagaagca aatatcggca
     9121 tggtcgataa aattttcacc cgtgtagggg ccagcgacaa tatttcttcg ggtgaatcga
     9181 cattcatggt cgaaatgaac gaaacagcga gcatcctgaa caatatcagt acccggagcc
     9241 tgatcctgct ggatgagatc ggccgtggca cgagcaccta cgacggcatc tcgatcgcct
     9301 gggcgatcac ggaatttctt catgagcacc ctgtttacag gccaaaagtc ctctttgcta
     9361 ctcattacca tgaattgaac gaaatggcgg ccactttcaa acggatccgg aatttccata
     9421 tttcggtgaa agagatcggc aaccaggtga ttttcctgcg aaaacttcgt aaaggaggca
     9481 gtgagcacag tttcggtatc catgtggccc ggctggccgg catgcccccg aaagtcatcg
     9541 aacgggccaa cgaactgctg aaagtcctgg aacgttccca cagcaacgat gaactggcca
     9601 aaatggccaa agagatcgga gcatccgaca aagaccagtt ccagctgtcg tttatccagc
     9661 tcgacgatcc gttgctggaa cagatcaaac aggatatttt gcataccgac atcgataatc
     9721 ttactcccgt agaggcattg atgaagctgc acacgatccg gaaacggctg agcggcgggt
     9781 aaaaaaattg tgggtgtctc a
//
LOCUS       DAOPNH010000112         9778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7559_length_9778_cov_4.668004, whole genome shotgun sequence.
ACCESSION   DAOPNH010000112 DAOPNH010000000
VERSION     DAOPNH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7559_length_9778_cov_4.668004"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /gene="ccoN"
                     /locus_tag="PKZ74_07295"
     CDS             complement(<1..1926)
                     /gene="ccoN"
                     /locus_tag="PKZ74_07295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HPM92603.1"
                     /translation="MEQEKFYYDNRIVKYFAYATLFWAIIGMLVGLLVALQFIFPSLN
                     FDLAYTTFSRIRPVHTNAVIFAFVGNGIFTGVYYSLQRLCKARMFNDRLSYIHFWGWQ
                     LIIVLAALTFIFGITTSKEYAELEWPVDILVALIWVIFGWNMFGTILKRRTRHIYVAI
                     WFYIATFVTVAVLHIVNSIAIPVSLLKSYPVYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYYLPKAANRPVYSYRLSIIHFWALIFLYIWAGPHHLLYNALPDWAQTLGMVFSL
                     MLIAPSWGGMVNGLLTLRGAWDKVREDPVLKFFVVAVTAYGMSTLEGPLMSIKAINAF
                     THFTDWTIAHVHVGALGWNGFLTFGMLYWLIPRMFNTQVFSKKLANVHFWLGTLGIIF
                     YAIPLYWGAVVQTLMWKEFDGLGFLKYPNFLETVTQIIPMYGARAIGGTLYVTGALIG
                     VWNLYKTIKAGQFSKETEATAPALVPVSSKRTDGESRHRWLERKPVQFMIASLIVILI
                     GGAVEIIPMYLIKTNIPTISSVKPYTPLELEGRDIYVREGCYTCHSQMIRPFRSETER
                     YGEYSKAGEFVYDHPFQWGSKRNGPDLAREGVKTGKIYKPDSWHFTHMIDPRSVSPGS
                     IMPTYPWLAENNLDVSYT"
     gene            complement(1971..2303)
                     /gene="ccoS"
                     /locus_tag="PKZ74_07300"
     CDS             complement(1971..2303)
                     /gene="ccoS"
                     /locus_tag="PKZ74_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HPM92604.1"
                     /translation="MNVIFILIAVSLLVAIGFLAAFIWTIRTGQFDDEYTPSVRVLFD
                     DEKNIIEENSKYHPPAGGPNAKQNSNSKSKRKINEQKQKQQPLTNQKPLTNKQEPGIW
                     NPEPGTRN"
     gene            complement(2412..4505)
                     /locus_tag="PKZ74_07305"
     CDS             complement(2412..4505)
                     /locus_tag="PKZ74_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPM92605.1"
                     /translation="MENLNRIRKGVLHSLVNFVKKEVTITFKEDELSLRELVELLVSL
                     NYVPQITLDDLYRKHARKGQRRIYYQLGVAGFCFGNIMLFSFPDYLAGSKPIDQDFIH
                     NFAYFNLVLAIPVVVFAARDYFISAWKGLMKKIVNIDVPIALGISVLFLRSAYDILAG
                     IDQGYMDSLSGLVFFLLIGKWYQGKTYQALSFERDYKSYFPVAVTILDDDGKESTIPL
                     KNLRPGNRIMVRNQELIPADAILVSGQGHIDYSFVSGESTPVHKQVKDRIFAGGRQVG
                     GMIELIVEKEVVQSYLTELWNQDLHRKTNSKGLNPVVDSVSRYFTLVIITISLGTGIF
                     WWMTDPSQAMNAFTSVLIVACPCALALTIPFTLGNTMRIFGRWGFYVKKTEVLEDLSR
                     IDTIVFDKTGTITQNDVFHVDFSQVELTEKEMALVRSLARHSSHPLSQAIYGSIKTGN
                     LLPVEDFSEQPALGLSGRFDGREVRMGSGLFVTGLEDETKNYSARVFVSIGGQTRGFI
                     TIRNEYRPGMEQVIGALRNRHELHLLSGDNDAEKGHLMPVFGDERSLHFRQSPAGKLE
                     YIKNLKDAGKKILMVGDGLNDAGALKESHVGITIADNVYHFSPACDGILDADRFGRLN
                     TFISFSRTAMKIVYAGFTLSFLYNIIGISFAAAGLLTPLLSAILMPLSSVSAVAFATL
                     ATWLLAKRRLRVLYI"
     gene            4605..4880
                     /locus_tag="PKZ74_07310"
     CDS             4605..4880
                     /locus_tag="PKZ74_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92606.1"
                     /translation="MIEISISAADLFFPDPDARMGFDVVICTGVALPEDFVNGFATVA
                     TKRFFIENDGIFPAIFPAVETVYFNTGIYVSYVPMWLINQFNLFILR"
     gene            complement(4853..7957)
                     /gene="ccsA"
                     /locus_tag="PKZ74_07315"
     CDS             complement(4853..7957)
                     /gene="ccsA"
                     /locus_tag="PKZ74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPM92607.1"
                     /translation="MMRKFFDFIFSMQFAGTLMLIFAAAVGTATFIENDFGTLAAKSV
                     VYSAKWFEALLALTAVSLIGSVFKYKLYERKRYSVLFFHLSFVVILIGAALTRYIGYE
                     GTMMIREGEASNQIISESPYVQVAVNKNGQSWNFDEEYLFTEYKKNRFRESFHFNDES
                     FKLQFKEYIPNAAEAVVEAKNGIPMMTFVTVDRGSRRTSLLKAGEVKTVKGLALSFNA
                     PTDDSTAIRINYSPETGLTVRTPFPSTVMNMMAGTTDTLRKDSTYTLFQMQLYDFMGM
                     QFVVRQFYPSGKTQLVSSPHESSGVAAIVMNLSYNGKTEEVGMLGGKGMVGQAVTTSI
                     DGTEFSLSYGSKYIGLPFYIKLRDFILERYPGSNSPSSFASEVTVIDPSENLEMPYRI
                     FMNNILNYGGYRFFQSSYDKDEKGTVLSVNSDYWGTLFTYIGYAILAAGLLYNFFSRN
                     SRFRKLARMTSELKKKSAMVVIAILIMVGFSAENAGAQHDGNFNPLNISKEHAKKFGE
                     LIVQDQDGRMKPVNTLSSELLRKIYRKDTYKGLNSEQVFLGIISNPEYWQGIPLIKVS
                     DDELKTAIGADGKYASFNDFLDNRQGFYKLGQHIEAANNKKPVERTSFDKDVIKVDER
                     VNIFYMAILGDFLTAFPEPGHPNHKWHTAEKPYETFDSTDAAFVENILPLYYGAVHDA
                     MVTNDWSQADTVLSYLKTFQQRYGEQVMPSSTKTRLEILYNNTNIFKRLFPIYGTIGF
                     IFIIALFTQVLSKRLNLKWIVRVLVGLIIACFALHTLGLAIRWYVAGHAPWSNGYETM
                     IYIAWGTVLSGILFLRKSPITTATTAILASVTLMVANLSWLDPQMTNLVPVLKSYWLV
                     IHVAVITASYSFLGVGALLGFLNMILIGLQTKNNYKKLKLTIEELTNINEMNLIIGVF
                     LITIGTFLGAVWANESWGRYWGWDPKETWALVTVLVYSFIIHMRLIPGLNGVYGFNMA
                     ALWGFSSVLMTYFGVNYYLSGMHSYAAGDPVPIPTFVYYSVAVAFLVSLLSYLNHLRM
                     KRLN"
     gene            complement(8094..9269)
                     /locus_tag="PKZ74_07320"
     CDS             complement(8094..9269)
                     /locus_tag="PKZ74_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92608.1"
                     /translation="MENYNLDKQLVISNRFNYLTYALMGIGVITIIVGLFVNPVKTWA
                     NYLMNNYYFLSLAIGATFFVALQYITQSGWSSAFVRIPQALSNVIPVIAVLMIPILVF
                     GLPQLYHWAHAGAADHDPIIAHKSPYLNLPFFIIRYVVFFAAWIFLTQYLKKLSLKED
                     RIGGMANFYKSELFSKIYIFTLAITFSLATFDWIMSVDVHWFSTIFAVRNFAMSFYHG
                     TVVITLVIIILNKLGYLPFLNKSHLKDFAKYIFILSIIWTYMWFAQYILIWYANIPEE
                     TVYYLPRTKGEFTPLFYLELFLNFAIPFALLLSNYLVSHKNTLLAICIVIIFGHWVDL
                     YLQIIVGTYGKLEIGMIEIGTFLGFAGMFAFIVARALKSAPLVAKNHPYLEESLSHH"
     gene            complement(9295..>9778)
                     /locus_tag="PKZ74_07325"
     CDS             complement(9295..>9778)
                     /locus_tag="PKZ74_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPM92609.1"
                     /translation="YAYMPDMYYSEAAEPYTENPLFRDSLTMQAPPKGTIARGHEPAY
                     PYLPRSADEQKRAGLELANPVPLTPEALAQGKEQYTIFCAMCHGDRGDGKGFLFTSKR
                     FPAQPTSLTGDLVKNKPDGEIYHVITLGTLSGLMGAHASQISPENRWKIVHYVRELGK
                     "
BASE COUNT         2446 a         2367 c         2544 g         2421 t
ORIGIN      
        1 cgtatagctg acatccaggt tgttttcagc cagccaggga taggtaggca tgatggagcc
       61 cgggctcacg gagcggggat caatcatatg ggtgaaatgc caggaatcgg gtttatagat
      121 ctttccggtc tttacgcctt cccttgccag gtcgggcccg ttccgtttcg atccccactg
      181 gaatggatgg tcataaacga actcgccggc tttggaatat tcgccgtagc gttccgtctc
      241 cgaacggaaa ggcctgatca tctgggagtg gcaggtatag catccttccc tgacatagat
      301 gtcgcgtcct tccagttcca gcggcgtgta aggcttcacg gaagagattg tcggtatatt
      361 ggtctttatg aggtacatag ggatgatttc aacagcgccg ccgatgagga tcacgatcag
      421 tgaggcgatc atgaactgaa cgggtttgcg ctcgagccag cggtggcgtg attcgccatc
      481 cgtgcgcttc gaagataccg ggacaagggc gggagccgtc gcttcggtct ctttgctgaa
      541 ctggccggct ttgatggttt tgtaaaggtt ccagaccccg ataagcgctc cggtcacata
      601 cagtgttccg ccgatagcac gcgcgccgta catcgggatg atctgtgtga cggtttccag
      661 gaagttgggg tatttcagga atcccaggcc gtcaaactct ttccacatca gggtttgaac
      721 cacagcgccc cagtacagcg ggatggcgta gaaaatgatc cccagtgtgc cgagccagaa
      781 atggacattg gccagtttct ttgaaaagac ctgcgtgtta aacatacggg ggatcagcca
      841 gtagagcata ccgaaggtca ggaagccgtt ccatccgagc gctccgacat gcacatgtgc
      901 gatcgtccag tcggtgaaat gggtaaaggc attgatggct ttaatcgaca tgagcggccc
      961 ttcgagggtc gacataccgt aagcggttac agccaccaca aagaacttga gcaccggatc
     1021 ttcgcggact ttgtcccagg ctccgcggag ggtgagcagg ccgttgacca ttcctcccca
     1081 cgacggggct atcagcatca gggaaaacac cattccgagg gtctgggccc agtcgggcag
     1141 ggcgttataa agcaggtggt gcgggccggc ccagatatac aggaagatca gcgcccagaa
     1201 atggatgatc gacagcctgt acgaatagac aggcctgttg gcggctttcg gaagatagta
     1261 gtacattagc ccgaggtacg gcgtggtgag gaaaaatgcc accgcgttat ggccatacca
     1321 ccactgaaca agggcatcct ggaccccggc atagaccggg taactcttca gcaggctgac
     1381 cgggatggcg atggagttga cgatatgcag gacggcaaca gtgacaaagg ttgcaatgta
     1441 gaaccagatg gctacataga tatggcgggt ccttcttttc aggatagtcc cgaacatatt
     1501 ccagccgaag ataacccaga tcagggctac caggatgtcg accggccatt ccagttctgc
     1561 atactccttg ctggtggtaa taccgaaaat aaaggtcagt gcggccagaa cgatgatcag
     1621 ttgccatccc cagaagtgga tatagctgag tctatcattg aacatacggg ctttgcaaag
     1681 gcgttgaagc gaatagtaaa caccggtgaa tatcccgttg ccgacaaagg caaatatcac
     1741 ggcattcgta tgcaccggcc tgatcctgct gaatgtggta taggccaggt cgaaattgag
     1801 tgatgggaaa ataaattgca aagccacaag caatccgaca agcattccga taatcgccca
     1861 gaacagcgtg gcataggcga aatacttgac gatcctgttg tcgtagtaaa acttttcctg
     1921 ttccataaat tgttgattgt tattggtttt tggttataag ttattagttc ttagttccgg
     1981 gttccgggtt ccgggttcca gattccgggt tcctgcttat tcgttaatgg tttttgatta
     2041 gttaatggtt gttgtttttg tttttgttca ttgatttttc ttttggattt tgaatttgaa
     2101 ttttgtttgg catttggtcc gccagctggc ggatggtatt tggaattttc ctcaataatg
     2161 tttttctcat catcaaaaag caccctgacc gacggtgtat attcgtcgtc gaactgaccg
     2221 gtacggatag tccagatgaa ggcggccagg aagccgatgg caaccagcag actgacggca
     2281 atgaggataa agatgacgtt cacggatggt taagtgaaat tggttccgga aaacaaatat
     2341 aaatgagaat aaggtaggat gacgggatca gtctgccgaa atatgtatgt ttattgatat
     2401 ttagaagggt tctaaatata taatactctc agccgccgtt ttgccagcag ccaggtggcc
     2461 aatgtcgcaa aagccacagc cgagaccgag cttagcggca tcaggatggc ggagagcagc
     2521 ggagtgagca ggccagccgc cgcaaatgat atgccgatga tgttgtaaag aaatgagagg
     2581 gtgaaaccgg cgtaaacgat cttcatggcg gtccggctga agctgatgaa ggtattcagc
     2641 ctgccgaacc ggtcggcatc aaggataccg tcgcaggcag gggagaagtg gtagacattg
     2701 tcggcaatag taatcccgac atggctctcc ttcagggcgc cggcatcgtt cagcccgtcg
     2761 cccaccatca gtatcttctt cccagcatct ttgagatttt tgatgtattc gagcttgccg
     2821 gccggcgact ggcggaaatg caggcttcgt tcatctccga aaacaggcat caggtggccc
     2881 ttctctgcat cattatcacc cgaaagcagg tgtaattcat gcctgtttct cagagcgccg
     2941 atcacttgct ccataccggg ccggtattcg ttgcggatcg tgatgaagcc tctggtctgc
     3001 ccgccgatcg agacaaatac gcgggccgag tagtttttcg tctcatcttc cagcccggtg
     3061 acaaacagcc ccgatcccat cctgacttcc ctgccatcaa accgtccgct cagccccagg
     3121 gccggctgct cgctaaaatc ctctaccggt agcaggtttc cggtcttaat actgccgtaa
     3181 atagcctggc tcagcggatg tgacgagtgc cgcgccagcg acctcaccag ggccatctcc
     3241 ttttctgtca gttcaacctg gctgaaatcg acatggaaga catcgttctg ggtaatcgtt
     3301 cccgttttat cgaaaacgat ggtgtcgatc cgtgaaagat cttccagtac ttccgttttc
     3361 ttcacataaa aaccccatct tccgaagatc cgcatggtat tgcccagggt aaaaggaatg
     3421 gtcagcgcca gggcgcaggg gcaggccacg atcagcaccg aggtgaaggc attcatcgcc
     3481 tgcgaaggat cggtcatcca ccagaatata ccggttccga gacttatggt gatgataacc
     3541 agcgtaaagt agcggctgac gctgtcgacc accgggttca gacctttgct gttcgttttg
     3601 cggtgcagat cctggttcca gagctcggta agataactct gtaccacctc tttctcaacg
     3661 atcagctcaa tcatcccgcc gacctgtctg ccgccggcaa agatcctgtc tttcacctgt
     3721 ttgtgaaccg gagtggattc gccgctgaca aagctgtaat cgatatgccc ttggccggaa
     3781 acgaggatag catcagccgg tatcagctcc tggtttcgga ccatgatccg gttgccgggt
     3841 ctgagattct tcagcggaat agtcgattcc ttcccatcat catccagaat ggtgaccgcc
     3901 acggggaaat aggatttgta atcacgttcg aacgacagcg cctggtaggt ctttccctgg
     3961 taccacttgc ctatcaggag gaagaagacc aggcctgaca gtgaatccat atacccctgg
     4021 tcgatcccgg ctaggatatc atacgccgat cttagaaata atacgctgat ccccagcgca
     4081 atcgggacat cgatattgac gatctttttc atcagccctt tccaggctga aataaaatag
     4141 tcgcgcgcag caaataccac aaccggaatg gccaggacca ggttgaaata ggcgaaatta
     4201 tgaatgaagt cctggtcgat gggcttgcta ccggccaggt aatcgggaaa gctgaaaagc
     4261 atgatattgc cgaagcaaaa accggcaacc ccgagctggt aatagatcct tcgctgacct
     4321 ttccgggcgt gtttcctgta aaggtcatca agagtgattt gcgggacata attcagcgaa
     4381 acaagcaatt cgaccagctc tcggagcgat aattcatctt ctttaaaagt aatcgtcacc
     4441 tcttttttta caaagttgac gagggaatga agaacgcctt tccggatccg gttaaggttt
     4501 tccaacagcc agatgcagga gctgcaatgt atggagggga tgaagaacct taccttgctg
     4561 atcccgctgt cggtgaattc cagtaaccct tccctgatct cctcatgatc gagataagca
     4621 tatctgctgc cgatctcttc ttccctgatc cggatgcccg gatgggcttc gatgttgtaa
     4681 tatgcacagg cgtcgctctc cctgaggatt tcgtaaacgg ttttgcaacc gtagcaacaa
     4741 aaaggttttt catcgaaaat gacgggatct ttcccgcaat cttccccgca gtggaaacag
     4801 tttattttaa taccggaatc tatgtgtcct atgtgcctat gtggttaata aatcaattca
     4861 accttttcat cctgagatga ttaagatacg agagcaatga aaccaggaat gcaacagcca
     4921 ccgaataata gacgaaagtc ggtatcggga ccgggtcacc ggcagcatag gagtgcatcc
     4981 ctgaaagata ataattcacc ccgaagtagg tcatcagcac cgagctgaat ccccacagag
     5041 ccgccatgtt gaagccgtaa accccattca gtccggggat caaccgcatg tggatgatga
     5101 atgaatagac cagaacggtc accagtgccc aggtttcttt cgggtcccag ccccagtagc
     5161 gcccccacga ttcattggcc cagacagctc ccaggaatgt gcctatggtg ataagaaata
     5221 ccccgatgat caggttcatc tcgttgatgt tggtcagctc ttcgatggtc agtttcagct
     5281 ttttgtaatt gttcttggtc tgaagcccga tcaggatcat attgaggaat ccgagcaggg
     5341 cccctacgcc gaggaagctg taactggccg tgatcaccgc cacgtggatg acgagccagt
     5401 aggatttgag aaccggcacc aggtttgtca tctgcggatc gagccagctc aggttggcca
     5461 ccatcagggt gactgaggcc aggatagccg ttgtagccgt ggtgatgggc gatttcctga
     5521 gaaagaggat gccgcttaaa accgtacccc aggcaatgta gatcatcgtt tcatagccgt
     5581 tgctccaggg ggcatggccc gccacatacc agcggatcgc caggcccagc gtgtgaaggg
     5641 caaaacaggc gatgatcaga ccgaccagaa ccctcacgat ccacttcagg ttaagtcgtt
     5701 tgctcaatac ctgggtaaaa agtgcgataa taaaaatgaa gccgatcgtc ccgtatatcg
     5761 ggaataggcg tttgaagata tttgtgttgt tatacaagat ctccaggcgg gttttggtgg
     5821 aggatggcat cacctgctct ccataacgtt gctggaaagt cttcagatag gatagcactg
     5881 tgtcggcctg cgaccagtcg ttggtgacca tggcatcatg aaccgccccg taatagagcg
     5941 gaaggatgtt ttcgacgaaa gctgcgtcgg tgctgtcgaa ggtttcataa ggcttttcgg
     6001 cggtgtgcca tttgtggttc gggtggcccg gctccggaaa tgccgtcaga aaatcgccca
     6061 ggatggccat atagaatatg ttcacccgct cgtcgacctt gatcacatct ttgtcaaaac
     6121 tggttcgctc aacaggtttt ttattgttag ccgcttcgat atgttgccca agcttgtaga
     6181 agccttgcct gttgtcaaga aaatcgttaa aagatgcata tttgccatcc gcaccaatag
     6241 ctgttttaag ttcgtcatca gatactttaa tgagcgggat gccctgccag tactccggat
     6301 tggagataat gccaaggaag acctgttcgc tgttcagccc tttgtaagta tctttccggt
     6361 aaatcttcct tagtaattcg gaagatagcg tattgacagg cttcattctc ccgtcctggt
     6421 cctgtacgat cagctctccg aacttcttgg cgtgttcttt actgatattc aagggattga
     6481 agttgccgtc gtgctgtgcg ccagcatttt ccgctgaaaa cccaaccatg atcagtatcg
     6541 cgatcaccac cattgcgctc ttctttttga gttctgaagt catcctggcg agcttgcgga
     6601 aacggctgtt ccggctgaag aaattgtaca gcagcccggc agcaaggatt gcataaccga
     6661 tataagtaaa cagcgtgccc cagtaatcgc tgttaacgga taagactgtc cctttttcat
     6721 ctttatcgta ggatgactgg aagaaacgat acccgccgta attcagaata ttgttcatga
     6781 agatacggta gggcatttcc aggttttccg aagggtcgat gacggtgact tcgctggcaa
     6841 acgacgaagg gctgttcgat cccgggtatc tttccaggat gaaatccctg agcttgatat
     6901 agaaaggcag gccgatgtat ttggagccgt acgacaatga gaattcggtt ccgtcgatcg
     6961 atgttgtcac cgcctggccg accattccct ttccgcccag catgccgact tcttctgttt
     7021 taccgttata ggaaaggttc atgacaatag ctgccacgcc gctcgattca tggggagaag
     7081 agaccagctg ggtttttccc gagggataaa actgccggac aacgaactgc atgcccatga
     7141 aatcgtacaa ttgcatctgg aaaagggtgt aggtagaatc tttccgaagg gtgtcggtag
     7201 taccggccat catattcatc acggtcgacg ggaagggggt acgcacagtc agccctgttt
     7261 caggtgagta attgatccgg atggctgttg aatcgtcggt gggagcatta aagctaagcg
     7321 caagcccttt gacggttttg acttcgccgg ctttgagcag gctggtccgg cggctgccac
     7381 ggtctaccgt gacaaaagtc atcatcggta tgccgttttt agcctctacc actgcttcgg
     7441 ctgcatttgg aatatattcc ttaaattgga gtttaaacga ttcatcatta aaatgaaacg
     7501 actcccggaa cctgttcttt ttatattctg taaatagata ttcctcgtcg aagttccagg
     7561 attgcccgtt tttatttaca gccacctgaa cgtaaggact ttccgagatg atctggttgg
     7621 atgcttcgcc ttcgcggatc atcatggtcc cttcataacc gatgtaccgt gtaagggctg
     7681 caccgatcag gataacgaca aaagaaagat ggaagaacag tacgctgtac cgtttccgtt
     7741 cgtatagctt gtatttgaat acgctgccga tgaggcttac ggctgtcaga gccagcagcg
     7801 cttcaaacca ttttgcgctg tatacgaccg atttagcggc aagggtaccg aagtcgtttt
     7861 caataaaagt agccgttccg accgccgccg caaagatcag catcaacgtg ccggcaaact
     7921 gcatggagaa tatgaaatca aagaatttgc gcatcataaa tcagggttca attgttcaat
     7981 tgttcgattg ttctcgtcca acagtcctat cgtactatag tctgcgatgc aaaaataacc
     8041 aaatcatcct tattataaag aatgagttta aataaatata gccttgcttg aaatcaatga
     8101 tgacttaagc tctcctcaag gtatggatgg ttctttgcca caagcggggc tgatttcaag
     8161 gcccgggcga caataaaagc aaacatgccg gcgaatccca ggaatgtgcc gatttcaatc
     8221 atgccgatct ccagcttgcc ataggtgccg acgatgatct gtaaataaag atccacccaa
     8281 tgcccgaata tgatcacgat acaaatcgcc agcagggtgt tcttgtggct gacaaggtaa
     8341 ttcgacagga gcagggcaaa cggaatggca aaattcagga acagctccag gtaaaacagg
     8401 ggagtgaatt cgcctttggt cctgggtaaa taataaactg tttcttccgg gatattggca
     8461 taccagatca ggatatactg ggcaaaccac atataggtcc agataatgct gaggataaaa
     8521 atgtatttgg cgaaatcctt caggtgcgat ttattcagga aaggcaggta tcccagcttg
     8581 ttcaggatga taatgaccag tgtgatcacc accgttccgt gatagaacga catggcaaag
     8641 ttcctgacgg cgaagatcgt actgaaccaa tggacatcta ctgacatgat ccagtcgaac
     8701 gtggccagtg aaaaggtaat ggccagggta aaaatgtaaa tctttgagaa cagctcactt
     8761 ttatagaagt tagccattcc gccgatccgg tcttctttca ggctcagttt tttaagatac
     8821 tgggtcagga agatccaggc agcgaaaaag acgacatagc gaataatgaa aaagggcagg
     8881 tttaagtaag ggcttttatg tgcgatgatc ggatcgtggt cggcggctcc cgcatgggcc
     8941 cagtgataga gctggggaag tccgaaaacc aggataggga tcatgagcac ggcaataacc
     9001 gggatgacgt tcgataacgc ctgggggatg cgcacgaacg cactcgacca ccccgattgt
     9061 gtgatgtact gtagagcaac gaagaaagtc gctcctatgg ccagcgacag gaaatagtaa
     9121 ttgttcatca ggtagttggc ccaggtttta accgggttga caaataatcc gacgatgatt
     9181 gtgatcacac cgatacccat cagggcatag gtcaggtaat tgaacctgtt tgaaatgacc
     9241 agttgtttat ccagattgta attttccatg tttgatcctc cttccgtctt cattctattt
     9301 acccaattcc ctgacgtaat gtactatttt ccagcggttc tcaggcgaaa tctgtgaagc
     9361 atgcgccccc atcaggcccg aaagagtgcc taaggtgatc acatggtata tttcgccatc
     9421 gggtttgttc tttaccaggt cgccggtaag cgaagtgggt tgtgccggaa accgtttgct
     9481 ggtaaaaagg aacccttttc catcgccccg gtcaccatgg cacatggcac aaaagattgt
     9541 atattgctct ttcccctgtg ccagtgcttc cggggtgaga ggaaccgggt tcgccagttc
     9601 cagcccagcc cgtttctgct catcggccga tctgggcagg taaggatagg ccggctcatg
     9661 gccacgggca atcgtccctt tcggaggcgc ctgcatcgtc aggctatccc tgaataatgg
     9721 gttctccgtg tagggttcag cagcctccga ataatacatg tcgggcatat aagcgtat
//
LOCUS       DAOPNH010000113         9707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7632_length_9707_cov_6.258392, whole genome shotgun sequence.
ACCESSION   DAOPNH010000113 DAOPNH010000000
VERSION     DAOPNH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7632_length_9707_cov_6.258392"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKZ74_07330"
     CDS             complement(<1..418)
                     /locus_tag="PKZ74_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HPM92610.1"
                     /translation="MKRLLLISNSTNPGEPYLGWPRPYITSFLAGTGVKNILFIPYAG
                     VNLSPEGLEKSYDIYEQRVQAVFKELGYGIYSIHHEQDPVEAVRKAEAIAVGGGNTFY
                     LVYMMHKMKLVDVIRDRANDGMHYMGWSAGSHPPVSS"
     gene            complement(453..3164)
                     /locus_tag="PKZ74_07335"
     CDS             complement(453..3164)
                     /locus_tag="PKZ74_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92611.1"
                     /translation="MTTYVKGNITAVVAALVFLFFADSYSYAQCRYSQEVNEEFRASG
                     ILRGPEHYYRNLIIQDIALARNVTPEEVSLEFHYNARFSVNRCTSGKLEITVAPPLFY
                     SPALFYHDFDISSYIRPSSGQMVFHLLQDKTIIADSIIFKNIIPNQDTGLYVSMDYTI
                     SSSVNDFQAVFSKARFFFDKSSYEQFRDQILHVDNFLAAVYVGRNLLDSIGTKDRSGV
                     QSKGDLILAWIIMDKVNRQMNPVNFRDLFVPVSHKSNALDSIARLLAIHQKRIGSILI
                     MNRKRPQGIISQVTSREELANLYFSHLDHYHDFAYSGDFRFVNFIEKLAEPVYSNAYL
                     LHLREFLAGQAGMDDAAIFSEGKRITGKMIRKGEELALAGDQLRAMKYFAASARLAGS
                     IRLYESFVYSRDKVAALKDSIAVSYVTISRKSASVNNPVLAVQYLEKAVNIYSNGIEA
                     KSQPGWLSEYRHELFRGFSLQIQALIKEEKYSKCMEYLVQIQNYCRNYPGFICPDEFH
                     DQMKTVRQGIYKELLNRSRLMLAAEEYNEAEKLLNEAVELRTAGGYNIGREIEESQLE
                     LSFRQIEYDLNIEEGYRFSDLHEYNSTLYYFNKAFAAGKSGIGKTDPDLDKNRLNAAR
                     QVIRQVLSEGRVKAWANDYEGAEEIIIRIETMLNDYHVATNDSLYAELALLKESVSGS
                     KCRKVQEQYRQKITQADSLIKAGNFIQARRLSQAAVDISFTNLDCMIRDEEAWYLKVW
                     LEVPAAYQEQEAGLERRLNGPWHDYLKAFEQLRNDYSRNKLLDYGIAFAPLIERIVAS
                     TDPDFLKDALFFYIELKDADNSFRVLKHIYSLGIPADPLREMQHKLGKLMAMRDYSVN
                     GSEKPWTTMESRQVSDKWFRDLKWSYKRTLLEKNRWKMKYWPVIWKN"
     gene            complement(3217..3300)
                     /locus_tag="PKZ74_07340"
     tRNA            complement(3217..3300)
                     /locus_tag="PKZ74_07340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3264..3266),aa:Leu,seq:cag)
     gene            complement(3312..3395)
                     /locus_tag="PKZ74_07345"
     tRNA            complement(3312..3395)
                     /locus_tag="PKZ74_07345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3359..3361),aa:Leu,seq:gag)
     gene            3566..5113
                     /locus_tag="PKZ74_07350"
     CDS             3566..5113
                     /locus_tag="PKZ74_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPM92612.1"
                     /translation="MFNQNDLDQIRARGISTDEIERQINDFKQGYPFAKLHAAATREN
                     GIRTFNEKEVHELITHFERTAKDMKLVKFVPASGAATRMFKHLYEFRERQEKVKKPGD
                     ILIDEDFNSVGYFFAHLNKFAFYDDLKRVMSRAGFNIEECIKRSDFDTVLDYLLTPAG
                     LNYGNLPKGVLKFHHYGTFSRTATGEHMVEGAFYAANRAKKVNVHLTVSPEHLSNFKD
                     LIAYVQPYYESMFGVSYDVSFSVQKPSTDTIAVDLNNEPFREKDGSLVFRPAGHGALI
                     ENLNDLDCDLVFIKNIDNIVPDRLKAETYRYKKLLGGLLLNLQVRSFQYLRALDKKPP
                     TEETIAEAVQFCEKQISISLPSGFEYWEESKKVGFLQQALNRPIRVCGMVRNEGEPGG
                     GPFWVKSKQGEISLQIVESSQIDMANPAQKKILQTATHFNPVDLVCSIRNYKGEKFDL
                     RQFVDPSTGFISVKSKDGRQLKALELPGLWNGAMANWITVFVEVPIITFNPVKTVNDL
                     LRPQHQN"
     gene            5165..5812
                     /locus_tag="PKZ74_07355"
     CDS             5165..5812
                     /locus_tag="PKZ74_07355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPM92613.1"
                     /translation="MTIIWDFNGTLLNDMQVCVDCMNILLEERALPPVGTEQYREIFT
                     FPVKEYYIHLGFDFEKEDFEVPAHRFIDLYRERLPNAPLHDEVKSVMDHFRKKGFRQV
                     VLSAMEQEFLDEAMRSKGIEHYFDRIAGITDHLGEGKNEMARSLIGTLPAAKSEIIMI
                     GDTLHDYEVSQANGIRCILITHGHQSPERLNGVECPKVSNFNELKDLIDRIGQLS"
     gene            complement(5813..6532)
                     /locus_tag="PKZ74_07360"
     CDS             complement(5813..6532)
                     /locus_tag="PKZ74_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92614.1"
                     /translation="MKRWILLALMIFATLFVRSQEMTDSHINKSDREGRRQGLWKVYD
                     EVGNLKYTGDYLDGKPIGTFTYYYPEGEVKALVRHSEGGGAAYVKNYHRNGKLMAEGK
                     YTDQKKDSTWLYYNETDGSLSLEEHYQNTVKEGVWKTFYPEGQVAEEVIYIHDRKDGP
                     WTQYFTDGKVKLKAAYVNDQIEGQYIVYHLNGMVEVSGFFQNSFKNGTWVYFNDKGEM
                     EKREEYQSGKLISQIVYDIEQ"
     gene            6602..7222
                     /gene="recR"
                     /locus_tag="PKZ74_07365"
     CDS             6602..7222
                     /gene="recR"
                     /locus_tag="PKZ74_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPM92615.1"
                     /translation="MNYSSKLLESAVNELSRLPGIGRKTALRLALHLVRQDEEAVVAF
                     SGAVLKLRQEIQRCSQCHNISDNEICDICANIKRDHQLVCVVQDIRDVMAIENTGQYN
                     GVYHVLNGIISPIDGIGPEDLTINSLLEKISREPVREVILALPTTVEGDTTNYYLSRK
                     LAPFQIILTAIARGIAIGDDIEFADEITLGRSIINRVPFTIPGSGV"
     gene            complement(7232..7474)
                     /locus_tag="PKZ74_07370"
     CDS             complement(7232..7474)
                     /locus_tag="PKZ74_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92616.1"
                     /translation="MPVVKSRKKPRKKVRYREVKLKLSRSQQKSLANYCSARGTTPTK
                     LIKKMIRPFLSNYSRAVPEELYVTANQLDLFGQENN"
     gene            complement(7547..8923)
                     /gene="glmM"
                     /locus_tag="PKZ74_07375"
     CDS             complement(7547..8923)
                     /gene="glmM"
                     /locus_tag="PKZ74_07375"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPM92617.1"
                     /translation="MTLIKSISGIRGIVGDGLTPPEIVRFTSAYVNYIRQENAEKRLK
                     IVVGRDARVSGQMVSAMVCSILMSCGADVIDLGLATTPTVEMAVKSYGADGGIIITAS
                     HNPAQWNALKLLNEKGEFLSAAAGETILQLAERNDFGYMGYRDIGTCQQDNNYLVTHI
                     NKILALPLVNKIAIERAGFRIAFDAVNSVGGLALPALLKALGVKEIIPLNANPDGLFA
                     HNPEPLPEHLAGICSLVKQTGADAGFVVDPDVDRLAIITETGGFFGEEYTLVAVADYI
                     LKHESGNTVSNLSSTRALKDVTERYGGQYFASAVGEVNVVEMMKKTRAVIGGEGNGGV
                     IYPDLHYGRDALVGIALFLSHLATSGTTCSDLRKTYPDYFISKNKVVLKPGSNPSDIL
                     VHIKKTYKDFPMNDSDGIRIDFPEGWVHIRQSNTEPIMRIYSEAAAMSVAEQLAARIR
                     MDISQWIC"
     gene            9034..>9707
                     /locus_tag="PKZ74_07380"
     CDS             9034..>9707
                     /locus_tag="PKZ74_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92618.1"
                     /translation="MESSFEHNETDLKALLERFEEMVRSDGAYFFDVEDLEDIVDYYL
                     DNQNLVKAKKAIDLAEAQHPGSTVFQLKKARYLILSEKTGKAISLLEEIEKIDPTNPD
                     IYMLKGSILSRLKKYDEAIGEYHRALFYSGDSEDIYNSIAFEYENAGNYDKAAEYLVR
                     ILEINPDNEAVIFELSFCYEITDNLEGAVDFLTQFLDRYPYNKIAWFNLGIAYNNLEL
                     FERAIEA"
BASE COUNT         2656 a         2294 c         2185 g         2572 t
ORIGIN      
        1 aagatgaaac tggtggatgt gatccggcgc tccatcccat gtaatgcatt ccatcattgg
       61 cgcgatcacg gatcacatcc accagtttca tcttatgcat catatatacc agataaaagg
      121 tattcccccc tcctactgcg atggcttctg cttttctgac agcttccacc ggatcttgtt
      181 catgatgaat agaatatatt ccatagccga gttctttaaa aacggcttgt accctttgct
      241 cataaatatc ataggatttt tcaagccctt ccggcgacag gttcacgccg gcataaggaa
      301 taaaaaggat atttttcaca cctgttcctg ccaggaagga cgtaatataa ggtctgggcc
      361 aacccaggta aggttcgccg ggattggtcg aattactgat caataataat cttttcataa
      421 tataatatta agggattggg aaacgaaact aatcaatttt tccaaatgac aggccagtat
      481 ttcattttcc agcggttctt ttccagcaag gtccgtttat agctccattt caggtccctg
      541 aaccatttat cgctgacctg gcgggattcc atggttgtcc aaggcttttc agaaccattc
      601 acactataat cgcgcatcgc cattaacttt ccaagtttat gctgcatctc ccttaacgga
      661 tctgcaggaa tgccgagcga ataaatatgt ttcaagaccc ggaaagagtt gtcggcatct
      721 ttcagttcga tgtaaaaaaa caatgcatcc ttaagaaaat ccggatcggt tgaggcgacg
      781 attcgttcaa tcaaaggggc aaaagcgatt ccataatcga gaagtttatt cctggaatag
      841 tcgttacgaa gttgctcgaa agcctttaga taatcatgcc atggcccgtt gagccttctc
      901 tccaagccgg cctcctgttc ctggtaagca gccgggacct ccagccagac tttgaggtac
      961 caggcttcct catcccggat catacaatcg aggttggtga aagaaatatc cactgctgcc
     1021 tggcttaacc tgcgggcctg gataaaattc cctgctttga tcagtgaatc agcctgagtg
     1081 atcttttgcc tgtactgttc ctgtacctta cggcatttgg agccgctaac agattcttta
     1141 agcaatgcca gttccgcata aaggctgtcg tttgtggcta catggtaatc attcagcatg
     1201 gtctcaatgc ggatgataat ctcttcggcc ccttcgtagt cgttggccca ggctttaacc
     1261 cggccttccg aaagcacctg gcggatcacc tggcgtgccg cattcaaacg attcttatcc
     1321 agatcaggat ctgttttgcc gatccctgat tttccggcgg cgaaagcttt attaaaatag
     1381 tacagagtcg agttgtattc gtgcaggtct gaaaaacgat aaccttcttc aatgttcaga
     1441 tcgtactcaa tctgacggaa tgaaagctcc agttgtgact cttcaatttc cctcccgata
     1501 ttgtaaccgc cggcagtcct cagctcaacg gcttcattca acagtttttc cgcttcattg
     1561 tattcttcgg cagccaacat caatctcgag cggttgagca actctttgta aatgccctgt
     1621 ctgaccgtct tcatttgatc atggaactca tcagggcaaa taaatccggg ataattccgg
     1681 caatagttct gtatttgaac aaggtactcc atgcatttgg aatatttttc ctctttgatc
     1741 aatgcctgga tctgaaggga aaagccccgg aacaattcat gacggtattc actcagccat
     1801 ccgggctgcg acttcgcctc tattccgttg ctgtaaatat ttactgcttt ctccaggtat
     1861 tggaccgcca gtaccggatt gttaaccgat gccgatttcc tggaaatagt aacatacgaa
     1921 acggcaatac tgtcctttaa tgcagcgacc ttatccctgg aataaacaaa tgattcatat
     1981 aaacggatac ttccggccag ccgggctgat gcagcaaaat atttcatcgc ccgtaattga
     2041 tcacccgcta aagccagttc ttctcccttt ctaatcattt taccggttat tctttttcct
     2101 tcgctaaaaa tagctgcatc gtccatgccg gcctgaccgg ccaggaattc cctcaaatga
     2161 agcaagtaag cgttactata gaccggttcg gcgagctttt cgataaaatt cacaaaccgg
     2221 aaatcgccgg aataagcaaa atcatgatag tgatccaaat ggctgaaata tagattggcc
     2281 agttcttccc ggcttgttac ctgtgatatt atcccctgag gccttttccg gttcataatc
     2341 agaatagagc cgatccgttt ctggtgaatg gcgagaagcc gggcaatgga atccagcgca
     2401 tttgacttgt gactgaccgg aacaaacaga tcccggaaat taaccggatt catttgcctg
     2461 ttaaccttat ccattatgat ccaggcaagg atcaggtctc cctttgattg tacccccgaa
     2521 cggtctttgg taccgattga atccagcagg ttgcggccca catatacagc cgcaaggaaa
     2581 ttatcaacat gcagtatttg atcccgaaac tgctcatagg aagatttatc gaaaaagaat
     2641 ctggctttag agaaaaccgc ctgaaaatca ttaaccgaag aagagatggt ataatccata
     2701 gatacgtaca agccggtatc ctggttgggt atgatatttt taaagataat actgtcggcg
     2761 ataattgttt tatcctggag aaggtgaaaa accatctgac ctgatgaggg gcggatatag
     2821 gatgaaatat caaaatcatg ataaaacaga gccggggagt aaaaaagcgg gggagctacc
     2881 gtaatttcaa gttttccgga tgtgcaccgg ttaaccgaaa agcgggcgtt gtagtggaat
     2941 tccagtgaga cctcctccgg ggtgacattt cgggccaggg cgatatcctg aatgatcaga
     3001 ttgcggtaat aatgttcagg gcctctcagg ataccggaag cccggaattc ctcatttact
     3061 tcttgagagt agcggcattg tgcataggaa taggaatcag caaagaatag aaaaacaagg
     3121 gcagcgacga cggcggtgat atttcctttt acgtaggttg tcaaaggtaa aggattaaat
     3181 taagatgccg gcctttcggc cggcatctga ttcgagtgcc caagacaaga ctcgaacttg
     3241 cacgggcgtt cgcccactac accctgaatg tagcgcgtct accagttccg ccacttgggc
     3301 attttgctcg gtgcccagaa caagactcga acttgcacga ccgagcggtc acatggccct
     3361 caaccatgcg cgtctaccac ttccgccatc tgggctaaaa gcggctgcaa aattaataat
     3421 ttatttgaat cctgaagatt tatttaagaa taatttcatt gaagataaaa agtaaaatat
     3481 ttgagattat tttcaaggat cactattttt acattttgaa agatgaagat taacttaacc
     3541 gatctcttat acaaagcaaa tgcatatgtt taatcagaac gacttggacc aaataagagc
     3601 ccgtggcatt tcaacagacg aaatagagcg gcaaatcaat gatttcaaac aggggtatcc
     3661 atttgcaaaa ctccatgcgg cagccacgcg tgagaatggc atcagaacgt ttaatgagaa
     3721 agaggtgcat gaactcatta cccactttga gaggacagca aaagacatga aactggtcaa
     3781 gtttgtcccc gcctccggag cggctacacg aatgttcaaa cacctgtatg aattccgcga
     3841 gaggcaggaa aaagttaaaa aaccgggaga tatattaata gacgaagatt tcaacagcgt
     3901 cggttatttc tttgcccatc tgaataaatt cgccttctat gatgatctta aacgggtgat
     3961 gtcgagagca ggatttaaca tcgaagaatg tataaaacga agcgacttcg atacagttct
     4021 cgactatttg ctcacaccgg cgggattgaa ctacggcaac ctgcccaaag gagtactcaa
     4081 atttcatcat tacggcacat tttcacggac agcaaccggg gaacatatgg ttgagggggc
     4141 attttacgct gccaaccggg cgaaaaaagt caacgtccat ctgaccgttt cccccgaaca
     4201 tctttcaaac ttcaaagacc tgatcgctta cgtgcagccc tactatgaaa gcatgttcgg
     4261 ggtcagttac gatgtcagct tctcggttca gaagccctcg acagatacca tagccgtcga
     4321 tcttaataat gagcctttcc gtgaaaagga cggatcgctt gtctttcgtc ctgccggaca
     4381 cggggcattg atagaaaacc tgaacgacct tgattgtgac ctggtattta ttaaaaatat
     4441 cgataacatt gtccccgacc gtttgaaagc agagacatac cgttataaaa agctactcgg
     4501 cggtttgctg ctgaacctcc aggtgagatc attccagtat ttaagggcac ttgacaagaa
     4561 accgcctacg gaagagacga tagcggaggc ggtgcagttc tgtgagaaac agatcagtat
     4621 atcattgcca tcaggctttg agtactggga agagagcaaa aaggtcggat ttctccaaca
     4681 ggctttgaac aggcctatcc gggtatgcgg aatggtcaga aatgaaggag agcccggtgg
     4741 cggcccattc tgggttaaaa gcaaacaggg ggaaatctcc ctccagatcg tggaatcctc
     4801 gcagatcgac atggctaatc ccgcacaaaa gaagatactc cagaccgcaa cacactttaa
     4861 cccggtcgac ctggtatgta gtatccggaa ctacaaaggc gagaaattcg acctgcgcca
     4921 atttgtcgat ccgagcacag gctttatttc tgtcaagtcg aaagacggca ggcaactgaa
     4981 agcactggag cttccgggac tatggaatgg cgccatggcc aactggataa ccgtgttcgt
     5041 ggaagtaccc atcattactt tcaacccggt aaaaacggtg aatgacctgc tccggcctca
     5101 acaccagaat taacccgggc tcttatcttc atccaaagtt gaaaacggga gaaataatca
     5161 cacattgaca atcatctggg attttaacgg gactttgctg aacgacatgc aggtgtgtgt
     5221 cgactgcatg aatattttgc tggaggaacg ggcattgccc ccggttggca cagagcaata
     5281 ccgggaaatc ttcacttttc cggtcaaaga gtattatatc cacctcggtt tcgattttga
     5341 aaaagaagat tttgaggtac cggcacaccg cttcatcgac ctttacaggg aaaggcttcc
     5401 gaatgcaccg cttcacgatg aggttaaatc tgtcatggat cattttcgta aaaaagggtt
     5461 ccgacaggta gtcctttccg ccatggagca ggagtttctc gatgaagcca tgcggtcaaa
     5521 aggaatagaa cattacttcg atcgcattgc cggtatcacg gaccacctgg gcgagggcaa
     5581 gaatgaaatg gcccgttcac tgatcggaac actcccggca gctaaaagtg aaataatcat
     5641 gatcggcgat accttgcacg attatgaggt ttcgcaggca aacgggatcc gatgcatact
     5701 gataacccat ggccatcaat cccctgaaag actgaacgga gtcgaatgcc cgaaggtcag
     5761 taattttaat gaattgaagg accttatcga ccggatcgga caattatctt aattattgct
     5821 caatgtcata aactatctgg ctgatgagct ttcccgactg gtattcttcc cttttttcca
     5881 tttcaccctt atcattaaag taaacccagg tgccattttt gaaggaattc tgaaagaacc
     5941 cggacacttc caccatcccg ttcaggtgat acacaatata ctggccttcg atctgatcat
     6001 taacataagc ggcctttaat tttactttgc catccgtaaa atattgcgtc cagggcccgt
     6061 ctttccggtc atgaatataa ataacctctt cggctacctg tccttcaggg taaaatgttt
     6121 tccaaactcc ttccttcact gtattttgat aatgctcttc cagggataac gatccgtctg
     6181 tttcattata atacaaccag gtactgtctt tcttctgatc ggtatattta ccctcggcca
     6241 tgagcttccc gttgcgatga tagttcttca cataagcagc tcccccaccc tcactgtgcc
     6301 tgaccagcgc tttgacttct ccttccgggt aataataggt aaaggtgcca atgggtttgc
     6361 catccaaata atcacctgta tatttaagat taccgacctc atcataaact ttccacaatc
     6421 cctgccgccg gccttcacgg tcggatttat tgatatggga atctgtcatc tcctgggagc
     6481 ggacaaaaag ggtggcaaat atcatcaggg ctaaaagtat ccagcgtttc atggttccaa
     6541 caattgatta attttgtcag aaaacgtaaa attaccattt ttattaaaca ccggaactaa
     6601 gttgaactat tcgtcgaaat tactcgaaag tgctgtcaat gaattgtcgc ggcttcccgg
     6661 aattggcaga aaaacagctc tccggctggc gcttcacctg gtccgccagg atgaagaggc
     6721 cgtggtggcg ttcagtggag ccgtgctcaa actcaggcag gaaatacaac ggtgctcaca
     6781 atgccataat atctccgaca acgaaatctg cgatatttgt gctaatataa aacgcgacca
     6841 ccaactggta tgtgtggtgc aggatatcag ggatgtgatg gccattgaaa ataccggcca
     6901 gtataacggc gtttatcatg tgctgaacgg gatcatttcc cctattgatg gaatcgggcc
     6961 ggaggatctg acaatcaatt cacttctgga gaaaatttcg cgggaaccgg tcagggaggt
     7021 cattctggcc ttgcccacaa ccgtcgaagg cgatacgacc aattattacc tgtcgagaaa
     7081 gttggcccca tttcagatta tactgactgc gattgcccgc ggaatcgcca ttggcgacga
     7141 tattgaattt gccgatgaaa taactctggg acgatccata ataaacaggg tcccctttac
     7201 catacccggt tcaggggttt aaaatagacc attaattatt ttcctgcccg aacagatcga
     7261 gctgattggc cgtcacatac aattcttcgg gcactgccct ggaatagttg gagagaaacg
     7321 gcctgatcat ctttttgatg agtttagtgg gtgtagtgcc ccgtgctgaa caataattgg
     7381 ccagcgattt ttgctggctc ctggaaagtt taagtttgac ttccctgtac cgcacttttt
     7441 tgcggggctt tttcctcgat ttgactacag gcatagattc cgtgattttc aggattctaa
     7501 aatagaatta tttaaagaaa ggatccgttg acagaaccga aatttattaa caaatccatt
     7561 gtgagatatc cattctgatg cgggcggcca gttgctcggc aacgctcatg gcagccgcct
     7621 cggagtatat ccgcatgatc ggttcggtat tggattggcg gatatgtacc catccttccg
     7681 ggaaatcaat cctgattcca tccgaatcat tcatggggaa atccttgtat gtctttttta
     7741 tgtgaacgag gatatcggaa ggattgctgc cgggcttcag aacaacttta ttttttgaaa
     7801 tgaaataatc cggatatgtc tttcgcaaat ctgaacaggt agtcccggac gtagccagat
     7861 ggctcaggaa caatgctatt ccaaccagcg catcgcgccc gtaatgcaaa tccggataga
     7921 tcacaccgcc gtttccctca ccgcctatca ccgcccgggt ttttttcatc atttcaacca
     7981 cattgacctc tcctactgcc gatgcaaaat actgaccgcc atatctttcg gttacatctt
     8041 ttaaagcccg cgttgaagat aaatttgaga cagtatttcc ggattcatgc ttcaggatgt
     8101 agtcggccac tgcgaccaaa gtatactcct cgccgaaaaa cccgccagtc tccgtgatga
     8161 tagccagccg gtcgacatcc ggatcaacca caaaaccggc atccgcaccg gtttgtttaa
     8221 cgagactgca gatccctgcc aggtgttcgg gaagcggttc gggattatgg gcgaaaagcc
     8281 cgtcgggatt tgcgttcagc ggtatgatct cctttacgcc caacgctttg agtaaagccg
     8341 gaagcgccag cccgcccaca gaattcacgg catcaaaagc tatcctgaaa ccggctctct
     8401 cgatagctat tttattgacc agcggtaatg cgaggatttt gttgatgtgt gttaccaggt
     8461 aattattatc ctgctgacag gtaccgatgt cgcggtatcc catgtatccg aagtcgttcc
     8521 tttctgccag ttgcagtatg gtttcgcctg cggcagccga aaggaattcg cccttttcgt
     8581 tcagtaattt caaagcattc cactgtgcag ggttatggct ggctgtaatg atgataccgc
     8641 catcggctcc gtaagatttt acagccatct ccacagtcgg cgtggttgcc agccccaggt
     8701 caatgacatc ggcgccgcag ctcataagta tactgcaaac catggctgaa accatttgcc
     8761 ctgataccct ggcatcacgg cctacgacga ttttcaggcg tttttccgca ttctcctgcc
     8821 ggatgtaatt aacataagcc gatgtaaacc tgactatttc cggtggagta agcccgtcgc
     8881 ccacaatacc ccggatgccg gatatggatt taatgagagt cattcaagat atttttggca
     8941 aaagtaatga agttagcgtg attgaagctg aaaacgacaa gtcggcagga caaagaaaat
     9001 atgtattttt gcggcaaatt tttcagtatt ttgatggaat cttcctttga gcataacgaa
     9061 acagacctga aagccttgct ggagcggttt gaggaaatgg tccgctcaga tggggcttat
     9121 ttttttgatg tggaagacct cgaagatata gttgactatt atctcgacaa tcagaatctg
     9181 gtcaaagcga aaaaagcgat agaccttgcg gaagcacagc atcccggttc cacggttttc
     9241 cagctcaaga aagcccgcta cctgattctt tcggaaaaaa ccgggaaagc catatcgttg
     9301 cttgaagaga ttgaaaagat cgatcctacc aatcccgata tctatatgct gaaaggttct
     9361 attctcagca ggctgaaaaa gtatgacgaa gcaatcgggg aatatcaccg ggcgcttttc
     9421 tattcgggcg attcggagga tatctacaac tcgattgctt tcgaatatga gaatgccggt
     9481 aattatgata aggcggccga atacctggtc cgaatcctgg agatcaaccc ggataatgaa
     9541 gcggttatat tcgaattatc gttttgttat gaaattaccg ataacctgga aggagctgtc
     9601 gatttcctca cgcagttcct tgaccgttat ccatacaata aaatagcctg gttcaacctg
     9661 ggtattgcct ataataacct ggaattattt gagcgggcca tcgaagc
//
LOCUS       DAOPNH010000114         9692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7655_length_9692_cov_6.998340, whole genome shotgun sequence.
ACCESSION   DAOPNH010000114 DAOPNH010000000
VERSION     DAOPNH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_9692_cov_6.998340"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..907)
                     /locus_tag="PKZ74_07385"
     CDS             complement(254..907)
                     /locus_tag="PKZ74_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92619.1"
                     /translation="MTFDLKLSNSIFKENLNSLLKISQDNYTLIKDQNQKYLSELIEK
                     QSSIVNQENNEFSEFTHLSNIDWILFNSIYIGVYSHFERHMFALARIIEDRSGSEIRI
                     NNFSGNGLVKYFNYIRLVGKIQSVTPDKDPWQQVMLHQKVRNLLVHNGGLMLNDISLK
                     LEKHECFNFLENSNVTMVGSFGHIRIKELDIIESIISTFKDVSDCLTNEIQFKYPEN"
     gene            1710..2006
                     /locus_tag="PKZ74_07390"
     CDS             1710..2006
                     /locus_tag="PKZ74_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HPM92620.1"
                     /translation="MERIFAKSTLREFWKSYPESEQFLKTWYDTAMNSIWKSPADIRK
                     TYATASILKDGRVVFNIKGNAFRLVAKINYEKQWIFIRFVGTHEDYDKIDATTI"
     gene            2020..2400
                     /locus_tag="PKZ74_07395"
     CDS             2020..2400
                     /locus_tag="PKZ74_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92621.1"
                     /translation="MKIRPVKNEEDYRKALKRLEVIFDAPSGTDEGDEADVLGLLIDD
                     YENKHYPIEAPDPIEAIRIRMEEMNLKQVDLIDEIGGKSRVSEILNRRRKLTLEMIRN
                     LNKRLNLSPELLISDYKLVKNQSR"
     gene            2397..3251
                     /gene="panC"
                     /locus_tag="PKZ74_07400"
     CDS             2397..3251
                     /gene="panC"
                     /locus_tag="PKZ74_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPM92622.1"
                     /translation="MKIFHTISETRAWLDEQKAAGKSSGFVPTMGALHRGHTELIRRS
                     VSENNITGCSIFVNPIQFNNPEDLEKYPRTLETDVAMLEEAGCDLVFVPSVKEMYPEP
                     VTEKYHFGPLEQVMEGAHRPGHFNGVAVVVKKLFDIFQPDRAYFGEKDYQQLAIIRAL
                     VKMKNIPVGIVPCPTVREADGLAMSSRNRRLTPEERAIAPEIYRTLDLAADLAGIKSV
                     AEVKQLACDRLTEKGFVVDYFEISDAVTLQPVDAWDQAPEIIGCVAAFLGNVRLIDNL
                     ILFRNFAG"
     gene            3290..3637
                     /locus_tag="PKZ74_07405"
     CDS             3290..3637
                     /locus_tag="PKZ74_07405"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPM92623.1"
                     /translation="MNVKLLKSKIHRATVTLSGLHYVGSIGVDEDLMDAAGLFNWEKV
                     HVFNINNGERFETYVIKEPRGSGTIGVYGAAARKVALGDLVIIAAYASMSPEEAKNFQ
                     PSIAIPDENNRLK"
     gene            3645..4658
                     /locus_tag="PKZ74_07410"
     CDS             3645..4658
                     /locus_tag="PKZ74_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM92624.1"
                     /translation="MKKRILSIIQYLFFLLIGFVLLWLVFRKIDITDVATEIRSAKYS
                     WLLLSITLGIISHIARAMRWNILIRSLGYKTDTQTTFYAVMVGYFFNMAFPRLGEVSR
                     CGVLSKKKGIPFNSLFGTVISERVFDTIVLAVIIVAVIFLQFSLLSNFVDKYIVTYFS
                     GIANRDNLVIAIILVALFIILPILLFRIFFHRIKEMHLYNKVKDFVMGLLAGITTIMK
                     IKQKWSFLGLTLVIWTLYTLMTFTAFFAIEATSSLSFIDAITVMSLGSLGMVAPVPGG
                     VGAYQFIVKAILVEIFLIAAEPAVSYSIILWSAQSLMIIVVGAISYYILVFKKSKVQH
                     EHA"
     gene            4618..5127
                     /gene="rfaE2"
                     /locus_tag="PKZ74_07415"
     CDS             4618..5127
                     /gene="rfaE2"
                     /locus_tag="PKZ74_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPM92625.1"
                     /translation="MSLRNRKFSMNTLENTRRKIAERADLSKLIAMWRFQNQTIVFTN
                     GCFDILHRGHIEYLSAAADLGNILIIGLNTDASVKRLKGEGRPVQDEQSRALALASLR
                     FVTAVVLFDEDTPYELIKSVQPDVLVKGGDYTEDTIVGAGIVRAHGGRVVTIPLTEGY
                     STSGILNSL"
     gene            5205..5456
                     /locus_tag="PKZ74_07420"
     CDS             5205..5456
                     /locus_tag="PKZ74_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HPM92626.1"
                     /translation="MGKNTSISLGDHFEDFITSKIEEGRYHSASEVIRAGLRLLEQEE
                     KKLNALKVTLELGEESGIAEEFNAKDYLKSLHKKHLKND"
     gene            5449..5754
                     /locus_tag="PKZ74_07425"
     CDS             5449..5754
                     /locus_tag="PKZ74_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM92627.1"
                     /translation="MIKYRLTNEAVKDLEEIWSYTCNKWSVGQADRYYHLIMEEIEFI
                     ASNPLSGKSVDYVKQGYRSAKVKSHLVFYHMTDENNILVVRILHQRMDIEDRIKIEK"
     gene            5780..7138
                     /gene="radA"
                     /locus_tag="PKZ74_07430"
     CDS             5780..7138
                     /gene="radA"
                     /locus_tag="PKZ74_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPM92628.1"
                     /translation="MTSKSKTVFFCQSCGSQSPKWIGKCPACGEWNTYVEEVVKVEGK
                     TVRREDGRTTKPVRVQEIKAEEGKRRTTHNGELDRVLGGGLVPGSIILVGGEPGIGKS
                     TLLLQVALRMKGQTVLYVTGEESAQQIRMRADRVGVKNDECFIMTETGTAQILHHLNE
                     IKPSMVIIDSIQTLQTDLLDSPAGSISQIRECAAEMQRFAKSTATPVILIGHIIKDGS
                     LAGPKILEHMVDTVLSFEGERNYGYRILRSVKNRFGPTSEMGIYEMHDSGLREVTNPS
                     EILISQREEDVSGVAIAASVEGLRPMLIEIQALVSSAAYGTPQRSTTGFDVRRMNMLL
                     AVLEKRSGFRLGAKDVFLNIAGGLRVDDPAIDLAVVAAILSSNADIPIDSKTCFAAEV
                     GLSGEIRAVNRIEHRVAEAEKLGFERIILSKYNKKALSGKKYQIELVFAGNVTDVVKW
                     IF"
     gene            complement(7297..8106)
                     /locus_tag="PKZ74_07435"
     CDS             complement(7297..8106)
                     /locus_tag="PKZ74_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92629.1"
                     /translation="MKRAGLILLMAGLILMAVVILLPGCKEDNEPAPSLPPQSSFVMD
                     FSDFSNPDDTLGTRGITTYQNWGYSYANVVIWQTFLTVGLAVPVAAFNESFNHEAVYH
                     PDEDNWTWSYNVTVGFVVYEAELTGYLVADSVAWEMRITKGSEFSDFLWYYGRSAIGG
                     SGGYWILQENPLNPNPLVLIDWHKYADGTAEISYTNIRPEVPENGTYIFYGTTLNDFN
                     RFYHIYNKVQDNLTEIEWSSVNKNGQVKDPLHFGDDLWHCWDGSLMDVVCP"
     gene            complement(8389..>9692)
                     /locus_tag="PKZ74_07440"
     CDS             complement(8389..>9692)
                     /locus_tag="PKZ74_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92630.1"
                     /translation="LTLLAFFFLLEIPRYKFTAIILGLLFILEVILLVRFVERTNRKL
                     TRFFESIRYADFSSSFSDPGLGKSFEDLSREFNQVIEAFRKYRTEKEEHFNYLQTVIQ
                     HVPIGIIVFQHDGKVEVFNNAMSKMLKVRNIRFVSDLKKIKDDLPPTLLNLKPGDNTL
                     YKVFSNDELLQIAMNATGFRMRNEDYMLVSMQNISAELEEKEIESWQKLIRVLTHEIM
                     NSITPITSLASTVHDMTVRREGGKIKRNEMDDEDLESFDAALTTIQNRSQGLLNFVQV
                     YRDLTRIPKPNFRYFRVQELFDRCNLLLKPKLDELGIGCTVRVFPPDLMITADPDLID
                     QVIINLMLNAIDAVKEAGNPAITVLATINSSNRVIIDITDNGCGIKPDIMDKIFMPFF
                     TSKKHGNGIGLSLSRQIMHLHKGSILFKSKPDEGTVFTLVF"
BASE COUNT         2540 a         2388 c         2348 g         2416 t
ORIGIN      
        1 gtattttttt atttcaattt ttatgtcaaa tatcagttat tagaggtccc cttaattatt
       61 gaaatctaaa aagttttaaa acagtcgtat cagagaatat tagttatgtt cgccgcgccg
      121 ctctggtttc ttctcctgag aacgtcatcc cgaagcgcag cgagggatca cccgccgccc
      181 gtccgccagc tggcggagca aaatttttaa ggaaaaactt gactttgcct tcgagcatcg
      241 ttagtggtag tttttaattt tctggatact taaattgaat ctcattagtt aagcaatcac
      301 tcacatcttt aaatgtgcta attattgatt caataatatc taactccttt attcttatat
      361 gcccaaaact accaaccata gtaacattac tgttctccaa aaaattgaaa cattcatgct
      421 tttctagttt taaggagatg tcattaagca ttaggcctcc attatggact aataaatttc
      481 tcaccttttg atgtaacata acttgttgcc aagggtcctt atctggtgta acagattgta
      541 ttttacctac aagcctgatg taattgaaat acttgacaag cccgttgcct gaaaaattgt
      601 taattcttat ctcagaaccg ctccgatcct ctataatacg ggctaatgca aacatatgtc
      661 tttcaaaatg agaataaact ccaatataaa ttgaattaaa aagtatccaa tcaatatttg
      721 aaagatgtgt gaattcagaa aactcgttgt tctcttggtt tacaattgaa gattgctttt
      781 caattaattc cgataagtat ttctgattct gatctttaat taaagtgtaa ttatcttgcg
      841 atattttgag caatgaatta agattctctt taaaaatcga attacttagc ttcaaatcaa
      901 aagtcatttt cgtaagcatt ttataattct aaaaattacc actaacgttt gtgcaagttt
      961 attgaattta gttccaatat atcaaactcc gggttgaata tacccctgct taattgagca
     1021 gtattattca atgaatgatg tttttgaaag actgatggta tgccttgctt tctcatacct
     1081 gattaatttg gccttcgtaa agataggaat tttaatattc aaatgatccg gcgggctttg
     1141 catttttccc caccataatg gcgtccctgg cgggacttct ccatgtctcc ttatctcgat
     1201 gctatactca aaatagaacc tcaatctctc tttgccatct attggcctgg tttgattgta
     1261 atcgttttca tattgattga tttttcacca cataggcaca gcagggcaca taggagacac
     1321 atagattatt attctggatt tccctatgtg gatctatgtg ctctatctgt ctatgtggtt
     1381 attttaaaac cctttccaga taggcacaac ttgtcccgaa acctcgggat aggacacata
     1441 gaaatcacaa tagctgtgac gcagagtttc gttaaggaga cacaaaggtt cgcaaagaac
     1501 ccttcgtggt ccttcgtgca ttcttcgtga aacttcgtgt aataattgtc aatccggaaa
     1561 agcaacaaaa aattcccggc cgctgccgga ttaatatgaa caacatcgga ggaggatatt
     1621 ccttatgatt tggggatttg gaattactaa aaaaataaag ttcccaattt gcgaatttat
     1681 tgtttatctt tgccttctgt ttaaaccgga tggaaaggat atttgctaaa agtacgctga
     1741 gggaattctg gaaatcatat ccggaatcgg aacagttcct gaagacctgg tatgatacag
     1801 ccatgaattc tatatggaaa tccccggcag atatccggaa aacatatgct acagccagta
     1861 tcctgaaaga tggaagggtc gtatttaaca tcaagggaaa tgcattcagg cttgtggcta
     1921 aaataaatta tgagaaacaa tggatcttca tcaggttcgt cgggactcat gaagattatg
     1981 acaaaatcga tgcgacaaca atttaataga aaggttatta tgaaaatcag gcccgttaaa
     2041 aacgaagaag actacaggaa agctttaaaa agactggagg ttattttcga tgccccttca
     2101 gggactgacg aaggcgacga agccgatgtg ctcggcctgt tgattgatga ttatgaaaat
     2161 aaacattatc cgatagaagc gcccgatcca atcgaggcga tcaggatcag gatggaggag
     2221 atgaacctta agcaggtaga tttgatcgat gaaatcggtg gaaaaagcag ggtttccgaa
     2281 attctgaacc gaagacggaa actgaccctg gaaatgatcc ggaatctcaa taaaagacta
     2341 aatctatctc cggagctatt aatttccgat tacaagctgg taaaaaatca atcccgatga
     2401 aaatcttcca tacaatctcc gaaacccgcg catggctcga tgagcagaaa gcggcgggga
     2461 aatcgtccgg cttcgtgccg acgatgggcg cgctgcaccg gggccatacc gaactgatcc
     2521 gccgctcggt cagcgaaaac aatatcaccg ggtgcagcat cttcgtcaac ccgatccagt
     2581 tcaacaaccc ggaagacctc gaaaaatacc cgcggacact ggaaaccgac gtggctatgc
     2641 tcgaagaggc aggctgcgac ctggttttcg tcccttcggt gaaagagatg taccccgaac
     2701 cggtgacgga gaagtaccac ttcgggccgc tggagcaggt catggaaggg gcgcaccgcc
     2761 cgggccactt caacggagtg gcggtcgtgg taaagaagct gttcgatatc tttcagcccg
     2821 accgggccta cttcggcgaa aaggactacc agcagctcgc catcatccgc gcgctggtga
     2881 aaatgaagaa tatccccgtc gggatcgtcc cctgcccgac cgtcagggaa gccgacgggc
     2941 ttgcaatgag ctcgcgcaac cgccgcctca cgcccgaaga aagagcgatc gcaccggaga
     3001 tctaccggac gctcgacctg gccgcagacc tggccggcat aaagagcgtc gcggaagtga
     3061 agcagcttgc ctgcgatcgc ctgaccgaaa agggctttgt cgtggattat ttcgaaattt
     3121 ccgatgccgt aacgctccaa ccggtggatg catgggacca ggcgccggag atcatcggct
     3181 gtgtggcggc tttcctgggc aacgtcaggt tgatcgataa tctgatttta tttcgtaatt
     3241 ttgcaggctg aattcaatta cccggcagag gcgggttaaa ctattgtaaa tgaatgtaaa
     3301 gttactaaaa tcgaagattc accgggctac ggtaaccctc tccggcctgc actatgtcgg
     3361 cagcatcggc gtggatgaag acctgatgga cgccgccggc ctcttcaact gggaaaaggt
     3421 ccatgtgttc aacatcaaca acggcgagcg ttttgaaact tatgtgatca aagagccgag
     3481 aggctcgggc accatcggcg tttatggcgc ggcagcccgc aaggtagccc tcggcgacct
     3541 ggtgatcatc gccgcctacg cctccatgtc gcccgaagag gcaaagaatt tccagccttc
     3601 tatcgctatt cccgacgaga acaaccggct aaaatgatat cgccttgaag aagcgcatcc
     3661 tgtccatcat ccagtacctt tttttcctgt tgatcggatt cgtgctgctc tggctggtct
     3721 tccgcaaaat cgatattaca gacgtcgcca ccgagatcag gtcggcgaaa tactcctggc
     3781 tcctcctctc catcaccctg ggcatcatca gccacatcgc ccgcgccatg cgatggaaca
     3841 tcctgatccg ctccctcgga tataaaaccg acactcagac caccttctac gccgtcatgg
     3901 tcggctattt cttcaacatg gccttcccga ggctgggcga agtgagccgc tgcggcgtac
     3961 tgagcaaaaa gaaagggatc ccgttcaatt cgctgttcgg taccgttatc tccgagcgcg
     4021 ttttcgatac catcgtcctg gccgtcatca tcgtcgcggt gatctttttg caattcagcc
     4081 tgctgagcaa cttcgtcgac aaatacatcg tcacctactt ctcgggcatt gccaatcgcg
     4141 acaacctggt catcgccatc atccttgtcg ccctgttcat catcctgccc atcctcctct
     4201 tccggatttt ctttcaccgg atcaaggaga tgcaccttta caacaaagtg aaggatttcg
     4261 tcatgggcct cctggccggg atcacgacga tcatgaagat aaagcaaaaa tggtcgttcc
     4321 tgggccttac gctcgtgatc tggaccttgt acacactgat gacctttacc gcctttttcg
     4381 ccatcgaagc cacctcatcg ctcagtttta tcgacgccat cacggtgatg tcgctcggca
     4441 gcctcggcat ggtcgcaccc gttcccggcg gcgtgggagc ataccagttc atcgtcaagg
     4501 cgatcctggt ggagatcttt cttattgccg ccgaaccggc cgtatcctac agtatcatcc
     4561 tctggtcggc ccagtcgctg atgatcatcg tcgtgggcgc catttcgtac tatatccttg
     4621 tctttaagaa atcgaaagtt cagcatgaac acgcttgaaa acacgcgcag gaaaatcgcc
     4681 gagcgcgctg acctgtcgaa actcatcgcc atgtggcggt ttcagaacca gacgatcgta
     4741 ttcaccaacg gctgcttcga catcctgcac cgcggccata tcgagtacct ctccgccgcc
     4801 gccgacctgg gcaatatcct tatcatcggc ctgaataccg acgcttccgt taaaaggctc
     4861 aagggcgaag gccgcccggt tcaggacgaa caatcgcggg cgctggcgct ggcttcgctc
     4921 cgcttcgtga ccgccgtggt gctgttcgat gaagacacgc cttatgaact gatcaaatcc
     4981 gttcagcccg acgtgctggt caaggggggc gattataccg aagacaccat cgtcggcgcc
     5041 ggcattgtgc gtgcccacgg cggcagggtg gttacgatcc cgctgacgga aggctattcg
     5101 acctccggaa ttttgaattc tctgtaatta cagtatttag gaattttttt aaggaaaatt
     5161 tataaaagta ccaaatattg gtacttttgt aaaaaacaac tactgtgggt aagaacacat
     5221 ccatatcatt gggcgaccat tttgaagatt ttattacgag taaaatcgaa gaaggcagat
     5281 accattctgc aagcgaagtg atccgtgctg gattacggtt acttgaacag gaagaaaaaa
     5341 aattaaatgc tctgaaagtt acgctggagc ttggcgaaga aagtggaatc gcggaggaat
     5401 ttaatgcaaa ggattacttg aaatctttgc ataaaaagca cttaaaaaat gattaaatat
     5461 cgtttgacca atgaagcggt aaaggatctt gaggagattt ggtcttacac ttgcaataaa
     5521 tggtctgtcg ggcaagctga cagatattat catttgatca tggaggaaat tgagttcatc
     5581 gcttcaaatc ctctttcagg taaatccgtt gactatgtaa aacaagggta cagatcagca
     5641 aaagtaaaat cacacctagt gttttatcat atgacagatg aaaataacat tttagttgtg
     5701 agaatacttc atcagcgaat ggatatagag gaccggataa aaatagaaaa gtgactttta
     5761 ataaaattct gaaattatta tgacctccaa atcgaaaacc gtcttcttct gccagtcctg
     5821 cggcagccag tcgcccaagt ggatcggaaa atgccccgcc tgcggcgagt ggaatacgta
     5881 tgtggaagag gtggtgaagg tggagggcaa gacggtaagg cgggaggacg ggaggaccac
     5941 aaaaccggtc agggtgcagg aaattaaggc ggaagagggg aagcggagga cgacgcataa
     6001 cggcgagctc gaccgcgtcc tgggcggcgg gctggtgccg ggatcgatca ttctcgtcgg
     6061 cggcgagccg gggatcggta aatcgaccct gctgctccag gtggcgctgc ggatgaaagg
     6121 acagacggtc ctgtacgtaa ccggcgaaga gagcgcacag cagatcagga tgcgggccga
     6181 ccgcgtcggg gtgaagaacg acgagtgctt tatcatgacc gagaccggca cggcgcagat
     6241 actccatcat cttaacgaaa taaagccgtc gatggtgatc atcgactcca tccagaccct
     6301 gcagaccgac ctgctcgact cccctgccgg cagcatctcg cagatcaggg agtgtgcggc
     6361 cgagatgcag cgcttcgcca aatccaccgc tacgccggtc atcctcatcg ggcatatcat
     6421 caaggacggc tcgctggcag gcccgaaaat actggagcac atggtcgaca cggtgctgag
     6481 tttcgaaggg gaacgcaact acggctaccg catcctgcgt tcggtgaaga accgcttcgg
     6541 gccgacgtcg gagatgggca tctacgagat gcacgacagc ggcctgcgcg aggtgaccaa
     6601 cccgtcggag atcctgatct cgcagcgcga agaagatgtc agcggggtgg ctatcgccgc
     6661 ctccgttgaa gggctgcggc cgatgctcat cgagatccag gcgctggtca gctctgccgc
     6721 ctacggcacg ccgcaacgat ccaccaccgg cttcgacgtg cgcaggatga acatgctgct
     6781 ggccgtcctc gagaaacgga gcggcttccg cctcggcgcc aaagacgttt ttctcaatat
     6841 cgccggcggc ctgcgcgtcg acgacccggc catcgacctg gccgtggtgg ccgccatcct
     6901 ctcctccaat gccgacatcc cgatcgacag caaaacatgc tttgccgcag aggtagggct
     6961 ttcgggcgag atacgcgccg tcaaccgcat cgagcaccgc gtcgctgaag ccgagaaact
     7021 gggcttcgaa cgcatcatct tatccaaata caataaaaaa gccctttccg ggaaaaaata
     7081 tcaaatcgaa ctggtgtttg ccggaaatgt gactgatgtg gttaaatgga tcttttaagt
     7141 aaacagcgca gtcctacgag cgtcccgtcg gttacttgaa agattctctt ccgacgactg
     7201 aagccgtcgg ttcctacatt cataaatcca taggaaccaa cggctttagt cgttggagcg
     7261 agaactaaga tcagggcagg ctgataggag gctgtctcac gggcatacaa catccatcaa
     7321 actcccgtcc cagcaatgcc acaggtcatc gccgaaatgc agcgggtctt tgacttggcc
     7381 gttcttattc accgaactcc attcgatctc cgtcaggtta tcctgaacct tgttgtaaat
     7441 atgatagaac ctgttgaaat cgttcagcgt ggttccgtaa aaaatatagg tgccattctc
     7501 gggaacttcg ggccggatat tggtgtaact gatctcggcc gtgccatcgg catacttatg
     7561 ccagtcgatc agaacaaggg gattgggatt caaaggattt tcctgcaata tccagtaccc
     7621 tccgcttccg ccgatcgccg atcttccgta ataccagaga aaatctgaaa attccgagcc
     7681 tttggtaata cgcatctccc aggcaacact gtcggcaacg agataaccgg tcagttcggc
     7741 ttcataaacc acaaaaccga ccgttacgtt atacgaccag gtccagttat cctcatcggg
     7801 atgataaacg gcttcatgat tgaatgattc gttgaacgcc gccacgggaa cggcaaggcc
     7861 caccgtaagg aaagtctgcc agatcactac gtttgcatag gaataccccc agttctggta
     7921 tgtggtgatc ccgcgggttc cgagtgtgtc gtcaggattt gaaaaatcgc tgaaatccat
     7981 gacaaaactc gattgcggag gaagcgacgg ggccggttca ttatcctctt tacaccccgg
     8041 aaggaggata actactgcca tcagtatcaa tccggccata agcaaaataa gacctgctct
     8101 tttcatatta tcactattaa ttagttgaat ttgattataa agataaacta ataacgtcca
     8161 ggaagcaaag agtattttca atcagtatct tccccctccc cttaatcccc tcccatgctg
     8221 acattatctt gaaaaacttg attatccgcg ggaggggaaa attgtgcggg tgccatcgcc
     8281 tttgctacaa atgtggaact cctccggagt aatgagaagc caatgtcatc caatattgtg
     8341 caatttgtta aagtctatat actcaggatg acgttccgtt ctaaaaaatc agaacaccag
     8401 cgtaaacacc gtcccttcat cgggtttgga tttgaacagg atgctccctt tgtgcaggtg
     8461 catgatctgt cgcgacaggc tcagcccgat cccgttcccg tgttttttgg aggtaaagaa
     8521 aggcatgaag atcttgtcca tgatatcggg tttgatcccg caaccgttat cggtaatatc
     8581 gatgatcacc cggttggaac tgttgatggt cgcaagcacg gtgatggcgg ggtttccggc
     8641 ttctttcacg gcgtcgatgg cattcagcat caggttgatg atcacctggt cgatcaggtc
     8701 gggatcggcc gtgatcatca ggtcgggagg aaagacacgc accgtgcatc cgatgccgag
     8761 ttcatccagc ttcggcttca acagcaggtt gcaccggtcg aacagctcct gcacacggaa
     8821 gtagcggaaa ttgggcttgg gaatgcgggt caggtcgcgg taaacctgca cgaaattcaa
     8881 cagcccctgg ctccggttct gaatggtggt aagggcagca tcaaagctct ccaggtcttc
     8941 atcatccatt tcatttcttt tgatcttacc gccctcccgt ctgaccgtca tatcgtgaac
     9001 tgtcgacgcc agcgaagtga tcggcgtgat cgagttcatg atctcatgtg taagcacccg
     9061 gatcagtttc tgccacgact cgatctcttt ttcctccagt tcggcgctga tgttctgcat
     9121 cgaaaccagc atgtaatcct cgttcctcat cctgaacccg gtagcgttca tcgcaatctg
     9181 gagcagctcg tcattggaaa aaaccttgta cagggtatta tcgcccggtt tcaggttaag
     9241 cagcgtcggg ggaagatcat cctttatctt ttttaaatcc gaaacaaacc ggatatttct
     9301 gaccttcagc atcttcgaca tggcgttgtt aaacacttcc actttaccgt catgctgaaa
     9361 tacgatgata ccgataggca catgctggat caccgtttgc aggtagttga aatgttcctc
     9421 tttttcggtc ctgtatttcc ggaatgcttc gatgacctgg ttgaattccc tggagaggtc
     9481 ttcgaagctt ttgcccagtc cggggtcgga gaaggagctg gaaaagtcgg catagcggat
     9541 gctttcgaag aaacgggtaa gtttcctgtt ggtacgctcc acaaacctga cgaggagtat
     9601 cacttccagg atgaacagca ggccgaggat gatggcggtg aacttgtacc gggggatttc
     9661 cagcaggaag aaaaaggcca gcagggtcag gg
//
LOCUS       DAOPNH010000115         9649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7694_length_9649_cov_7.845528, whole genome shotgun sequence.
ACCESSION   DAOPNH010000115 DAOPNH010000000
VERSION     DAOPNH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7694_length_9649_cov_7.845528"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKZ74_07445"
     CDS             complement(<1..1012)
                     /locus_tag="PKZ74_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPM92631.1"
                     /translation="MSDRLVHTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVDDG
                     TATMDWMIQEQERGITITSAATTVFWKVDGQDYKVNIIDTPGHVDFTVEVERSLRILD
                     GAIAIFCAVGGVEPQSETVWRQADKYQVPRLAYVNKMDRSGADYFRVLQQIKAKLKAN
                     PVPIQLPIGVEDTFTGVVDLIENKAYTWEEESFGLNYHETEIPADMADMVREYRTSLV
                     EGAAEESEELLEKFLENHDSITAEDIHLALRKATLEGRIVPVLCGSSFHNKGVQKLID
                     AIVRYLPSPLDIPPVEGTNPFTDKKETRRADENEPFTALAFKIATDAFVGKIAYFRVY
                     SGQ"
     gene            complement(1054..1521)
                     /gene="rpsG"
                     /locus_tag="PKZ74_07450"
     CDS             complement(1054..1521)
                     /gene="rpsG"
                     /locus_tag="PKZ74_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPM92632.1"
                     /translation="MRKGKSKSRIIVPDPKFGDLMVTKFVNNIMLRGKKNAAFDVFYE
                     AIEIVAQRSKEDGLEVWKKALANVTPAVEVRSRRVGGATFQIPSEIRPARKQSIGMKN
                     MIKFARNRHEKSMQEKLAGEIMAAYKEEGSAFKKKEDMHRMAEANKAFSHFRF"
     gene            complement(1541..1915)
                     /gene="rpsL"
                     /locus_tag="PKZ74_07455"
     CDS             complement(1541..1915)
                     /gene="rpsL"
                     /locus_tag="PKZ74_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPM92633.1"
                     /translation="MPTIQQLVRKGRAKLIDKSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGAL
                     DTSGVDGRKQRRSKYGAKRPKK"
     gene            2254..2916
                     /locus_tag="PKZ74_07460"
     CDS             2254..2916
                     /locus_tag="PKZ74_07460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPM92634.1"
                     /translation="MKEQYFSLQGLQKETEEMILEITSGRLKSNPRKWNADISRSALI
                     VTDMQNYFLNPDSHAFIPSATSIIPNILSLADFFHKSNRPVIFTKHTNDHENAACMSY
                     WWKDMIGNETPSAKIHENLRTSGDIILEKHQYDAFYNTRLEEILHNQNVRFAVICGVM
                     TNMCCETTTRSAFVKGFQPLLPLDATAAYNREFHISTFINLSFGFCPLMTTREVIEVL
                     MK"
     gene            2913..3800
                     /locus_tag="PKZ74_07465"
     CDS             2913..3800
                     /locus_tag="PKZ74_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM92635.1"
                     /translation="MTENKIIIIGYGPSGIACAIQMVRLGLHPVVIEKSRPGGMLTNA
                     RLIENYPGFPEGITGEKLARRMIKQAKRFNINVIKDEITFVDHRNDNYILKGIIENYT
                     CDVLVAATGTVPVTPENIPAKLFRNKRIHTDIAGLKNISGKSVGIVGAGDAAFDYSLS
                     LAEKENNVMIFNRGDQVKALKVLKENVFINNRINYIDNIEIKSLEILPDKRLHATCNS
                     ASAIRNYFLDYLIFATGRKPADGFFQKSLEGKLDGLISSHRLYLIGDLKNGSCRQVSA
                     AVGDGVRAAMEIFHHESNQ"
     gene            3784..4719
                     /locus_tag="PKZ74_07470"
     CDS             3784..4719
                     /locus_tag="PKZ74_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92636.1"
                     /translation="MKVISKTENSQLATVYIAQNSSGKLIEFVESVQPPLTLSEKWVL
                     IVSTLYGCPVDCSFCDAGGNYHGRLTADEILFQVDYPVRLRFPDGVIGTEKFKIQFSR
                     MGEPSFNPAVLTVLEKIPQLYTIKEFIPSLSTIGPNGTDKFFGRLLEIKKELYPKTFQ
                     FQFSIHTTSLKQRDQLIPVRKWDFRQMARYGEKFFDEEGKKITLNFAVSRESIIDPDV
                     LTRHFSPDIFLIKLTPVNPTFKARKNQIESLDLKDEGFYSDVIQNIREAGYETIVSIG
                     EMEENLIGSNCGQYVQAHLKSCEKMPEAYQYQLNQ"
     gene            5148..5600
                     /gene="tnpA"
                     /locus_tag="PKZ74_07475"
     CDS             5148..5600
                     /gene="tnpA"
                     /locus_tag="PKZ74_07475"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPM92637.1"
                     /translation="MASTYISVNIHYVFSTKGRKNLISKEIKPRLWQFMGGIARKNKM
                     VAYAVGGTENHVHVLLSIPATINIAEAIKLIKGGSSKWVNDEFIVPGGFKWQEGYGAF
                     SVSQRGLQRTIAYILNQEDHHRKKSFRDEYVDFLNQCGIQYDERFLLG"
     gene            5976..8294
                     /locus_tag="PKZ74_07480"
     CDS             5976..8294
                     /locus_tag="PKZ74_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM92638.1"
                     /translation="MESLLKDLNEPQKNAVINYEGPSMVLAGAGSGKTRVLTYRVAYL
                     VHKGIDPFHILALTFTNKAAREMKERITSLVGADARNVWMGTFHSIFSRLLRIDGYRL
                     GYTPQFTIYDTEDSKRTIRNIVKEMDLDPKVYQPGYILHRISSAKTRMMSAREYFDDP
                     EIKAADQSSGKPHIGQIFLSYAERLKKSDAMDFDDLLVNMNILLEHYPEVLLKYQRKF
                     EYILVDEYQDTNYCQYLILKHLAANNENICVVGDDAQSIYAFRGASIQNILNFRHDYP
                     DHRIFKLEQNYRSTQNIVNAANSIIINNKDQIEKKIWTQNDTGPKIRLIRAKSDTEEG
                     TLVVNAIFELKMNKQLGNDAFAVLYRTNAQSRSFEESLRKLNIPYRIYGSVSFYQRKE
                     IKDLLAYFRLAINNRDEDALIRVINYPARGIGQTTMEKIIVTAGQHQVSSWQVIENPD
                     YYQLAVNSSTRHKLEEFVVMIKSFSARMKAKDAYDLARTVATTSGILKDLYEDQTPEG
                     VSRFENVEELLNAIREFCDTPRPEPVMIEDDTPLPDLPAFRTLDEFMQDVALITDADD
                     KDPSDRNHVSLMTIHAAKGLEFPHIFISGLEENLFPSIQSLNSRADLEEERRLFYVAL
                     TRGMHSVTVSYAENRYRWGNLEPCDPSRFIDEIDPKYIEIPRKTSMYDLPVDFRKIRG
                     ADSRETAPGKTDPPRKLKKLTNFTNYSSGDSGKQDELSGLQPGMMVEHEKFGKGKVQS
                     VEGEGSNKKAVIFFQLIGQKQLLLKFAKLKIL"
     gene            8433..9095
                     /locus_tag="PKZ74_07485"
     CDS             8433..9095
                     /locus_tag="PKZ74_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPM92639.1"
                     /translation="MEYNTQKNKLIIAEYGRNLQQMAMDCANIEDREKRTRIAEFIVN
                     VMAQMNPKVKESGEYKQKLWDHLYIMSDFKLDVDSPYPPPSREVLLFAHKSIKYQQSN
                     IRYRHYGKNIEMIIDKAVTYDEGPEKEALIKTIANHLKKSYLNWNRNSVDDEVILKNL
                     GELSGNRLKLSEEHKLSHTNEILARNIVKKKKFVKPGTGNQFGAPGNQGQPYHKLKKK
                     QG"
     gene            9092..>9649
                     /locus_tag="PKZ74_07490"
     CDS             9092..>9649
                     /locus_tag="PKZ74_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPM92640.1"
                     /translation="MSSFEIHGGKRLNGTIRPQGAKNEALQILCAVLLTPEKVTVSNV
                     PDIRDVNFLIDLLRDMGVSVNKTAPETFEFQADQINLEYLRTPQFKQKAASLRGSVMI
                     LGPLLSRFRKGYIPHPGGDKIGRRRLDTHFIGLQNLGARFIFNEKEKLYEVEAEDLKG
                     CYMLLDEASVTGTANIIMAAVMAKGK"
BASE COUNT         2749 a         2309 c         2153 g         2438 t
ORIGIN      
        1 cctggcctga atagacccgg aaatatgcta ttttgccgac gaaagcatcg gttgcaattt
       61 tgaaagcaag cgctgtaaaa ggctcgttct cgtcagcccg gcgtgtttcc tttttatcag
      121 tgaatggatt ggtaccttcc accggcggaa tgtcaagagg gctgggtaga tatctcacaa
      181 tggcatcgat cagtttctgg acacctttat tatggaaaga tgatccacac agtaccggga
      241 caattcttcc ctcaagagtg gctttgcgca aagcgaggtg tatatcttca gccgtaatgg
      301 aatcgtgatt ctcaaggaac ttttcaagaa gttcttcgct ctcttcagcc gcgccttcca
      361 ccaggctggt gcggtattca cgaaccatat ccgccatatc tgccgggatt tccgtttcgt
      421 ggtagttcag gccaaaggat tcctcttccc atgtataggc cttattttca atcagatcaa
      481 ccacaccggt aaaagtatct tcaacgccga tagggagttg aatgggtacc gggttagcct
      541 ttaacttggc tttgatctgc tgcagcacac ggaaatagtc ggcaccggaa cggtccattt
      601 tgttcacata agccaaccgg ggaacctggt atttatccgc ctggcgccat acagtctccg
      661 actggggttc aaccccgccg actgcgcaga aaatggcaat ggctccatca agaattcgga
      721 gtgatcgctc cacttccacg gtgaaatcca catgtcccgg ggtatcgatg atattgactt
      781 tataatcttg tccgtcgact ttccagaaaa cggtggttgc ggccgaagta atcgtaatcc
      841 ctctctcctg ctcctggatc atccagtcca tggtcgccgt cccgtcatct acttcgccga
      901 tcttgtaatt gatgccggta tagtacaata tacgctccgt cgtcgtggtc ttgcccgcgt
      961 cgatgtgcgc catgatgccg atgttccggg tatgtactaa cctgtccgac atttttttgt
     1021 actctatacc tgattcaaat taatactcaa aacttaaaat ctgaaatggg agaaagcctt
     1081 gttggcttct gccatccggt gcatatcttc tttcttttta aaggctgaac cttcttcttt
     1141 ataggcagcc attatctcac cggccagttt ttcctgcatc gacttttcat gacgattacg
     1201 ggcaaacttg atcatgtttt tcataccgat ggattgcttt cgggcaggcc tgatctccga
     1261 agggatctgg aatgtggctc caccaacccg gcggcttctg acctcaacgg caggcgtaac
     1321 attggccagg gctttcttcc acacctcaag accgtcttcc ttgctgcgct gggcaactat
     1381 ctcgattgcc tcgtaaaaaa catcgaaagc ggcatttttc ttaccacgca acataatatt
     1441 gttgacaaac ttggtcacca tcaggtcgcc gaattttgga tcgggaacga tgattctcga
     1501 ttttgatttg ccttttctca tcagttattt gatataaata ttacttctta ggtctttttg
     1561 ctccgtactt tgatctgcgc tgctttctgc cgtcgacacc ggatgtatcg agagcgccgc
     1621 ggatgatgtg gtaacgtaca cccggcaagt ccttcaccct gccaccacgt atcagcacaa
     1681 tgctgtgttc ctgcaggtta tggccttctc cgggaatgta cgagttgact tcttttccat
     1741 tggtcagtcg taccctggcc actttacgca tggcggaatt cggcttcttg ggagtggtcg
     1801 tgtaaaccct tacacaaaca ccacgccgct gaggacatga atccagtgcg ggagatttgc
     1861 tcttatcaat caatttagca cgtccttttc tgacaagctg ttgaattgtt ggcatacgct
     1921 ctttattaaa attttaattt gctttttgct tccttttttt acagacacaa tatgttgtgt
     1981 ctttaccctc tcattgttca ggatttttca ggaaagtata caaggcgggc tcttacggag
     2041 actgagcggc tttctttttc acgccctgaa cctatatcgt ctcaccttgt caggttccta
     2101 agtctttctg gataagctca gaacgactgt ccttaaaatt cgggctgcaa aggtataaat
     2161 tgattttgaa ttaacaaacg tttatggaaa tatttttttt gaggaggaaa gcttaactct
     2221 gcgttgcgaa attaaaaaat atatctttat tccatgaaag aacagtattt ctcattacag
     2281 ggcttacaaa aagaaactga agaaatgatc ctggaaataa ccagcgggcg tttgaagagc
     2341 aatccccgaa agtggaatgc cgatatcagc cggtctgccc ttattgttac cgatatgcag
     2401 aattatttcc tcaatcctga ttcccacgct tttatcccct cagcaacatc cattatacct
     2461 aatatcctga gcttggccga cttttttcat aaaagtaaca gaccggtcat ctttacaaaa
     2521 catactaatg accatgaaaa tgcggcatgc atgtcgtact ggtggaaaga catgatcgga
     2581 aacgagactc catctgctaa aatccatgaa aacctgcgaa cttccggtga tatcatcctt
     2641 gaaaaacatc aatacgatgc tttctacaat accaggcttg aagaaatact gcataatcaa
     2701 aacgtgcggt ttgccgtgat ttgcggggta atgaccaaca tgtgctgtga aacaactacc
     2761 cgaagtgcat tcgtaaaggg tttccaaccc ttattgcctt tagacgccac ggccgcatac
     2821 aaccgggagt ttcacatttc cacttttata aacctgtcct tcggtttctg cccgctgatg
     2881 accacccggg aagtcataga agttctcatg aaatgactga aaataaaatc attatcattg
     2941 gttatggtcc atctggcatt gcctgtgcaa ttcagatggt ccggttggga ctccatcccg
     3001 tggtcatcga aaaaagccgg cccggaggca tgctcaccaa tgcccgcctc atcgaaaact
     3061 atccgggttt cccggaagga ataaccggtg aaaaattggc ccggcgtatg atcaagcaag
     3121 ccaaacggtt taatatcaat gtaataaaag atgaaattac ctttgtcgat caccggaatg
     3181 ataactacat attaaaaggt ataatagaaa attatacatg cgatgtactt gtcgcggcca
     3241 caggaacggt accggtgaca cctgagaata taccggcaaa gcttttccgc aacaaacgaa
     3301 tacataccga tattgccggg ctgaaaaaca tttcggggaa gtcggtcggt atcgtcggcg
     3361 ccggcgacgc tgccttcgat tacagcctct cattagccga aaaagaaaat aacgtaatga
     3421 tcttcaaccg gggagatcag gtcaaggcat tgaaagttct taaggaaaat gtatttatca
     3481 acaataggat aaattacatt gacaatattg agataaaaag cctggaaatc cttcctgata
     3541 aacgtttgca tgccacctgc aacagtgcgt ctgcgattag aaattatttc ctggattatc
     3601 ttatttttgc gaccggaaga aaaccggctg acggtttctt tcaaaaaagc ctggaaggca
     3661 agctggacgg gctgataagc agccaccggc tctacctgat cggcgacctg aagaacggct
     3721 cttgtcgtca agtctcagca gccgttggcg atggagtaag agcggcaatg gaaatattcc
     3781 accatgaaag taatcagtaa aacagaaaac agccaactgg caacagttta tattgcgcag
     3841 aattcttcag gaaaactgat agaattcgtc gaatctgttc aaccccccct tacactttcg
     3901 gaaaaatggg tactgattgt atcaaccctt tacggatgcc cggttgattg ctccttttgc
     3961 gatgcgggcg ggaattatca cggcaggctt acagccgacg aaatactctt ccaggtcgat
     4021 tatccggttc ggctcagatt cccagatggt gtgatcggaa cggagaaatt caagatccag
     4081 ttctcccgga tgggcgaacc gtcatttaat cccgctgttc tgacggtact ggaaaaaatc
     4141 cctcaacttt ataccattaa agaattcatc ccttcccttt caacgatagg cccgaatggt
     4201 accgataaat ttttcggacg gctccttgag atcaagaaag aattgtaccc caaaaccttt
     4261 caattccagt tttcaatcca cacaacttcg ctgaagcaaa gggaccagct gattccggta
     4321 agaaaatggg acttcagaca aatggcccgg tacggcgaga agttttttga tgaagaagga
     4381 aaaaagatca ccctcaattt cgccgtgtcc agggagagta ttattgaccc agatgtactg
     4441 acccgtcatt tttccccgga tatctttcta attaagctca ccccggtaaa cccgactttc
     4501 aaggccagaa aaaaccaaat agaatctttg gatcttaagg atgaaggttt ttattctgat
     4561 gtaattcaga atatcaggga agccggatac gaaaccattg tgagtatcgg cgaaatggaa
     4621 gagaacctta tcggcagcaa ttgcgggcaa tatgttcagg cgcatttgaa atcatgcgaa
     4681 aaaatgccag aagcctacca gtatcaatta aatcagtaaa aaaattccaa acgccgggga
     4741 atcgatacct ttttttaaga tatccctccg ggacacctga aaatcccgaa gggatttccg
     4801 atagtagtcc gccgatttat cagagaacgg acatcggata atcccgaagg gatttccgat
     4861 aataacccgc cgatttatcg gagaggcaca gaacccggga tgtacccgtc atgtccggaa
     4921 tagacatcgg ataatcccga agggatttcc gataataacc cgccgattta tcggagaggc
     4981 acagaacccg ggatgtaccc gtcatgtccg gaacggacac ttgataatcc gtccggattt
     5041 caatccggtc aacaaatcca aaatattttt ttgaaccatc gggtattttt caataaaaaa
     5101 ccggattaag cagtatagat catttcttaa tctcaaaatc atacattatg gcatcaacgt
     5161 atatctcagt gaacattcac tacgtgttct ccacaaaagg aagaaagaat ctgatctcga
     5221 aggagatcaa gccccggtta tggcagttta tgggaggaat tgcaaggaaa aataaaatgg
     5281 ttgcatatgc agtaggcgga acagagaatc acgttcatgt gctgctctca atcccggcaa
     5341 cgattaatat tgccgaggca attaaactga tcaaaggcgg atcatcaaaa tgggttaacg
     5401 atgaatttat tgtacccgga ggatttaaat ggcaggaggg atacggcgca tttagtgtca
     5461 gccagagggg gctgcaaaga accattgctt atatcctgaa ccaggaagat catcacagga
     5521 agaaatcttt cagggatgaa tatgtcgatt ttttaaacca atgtgggatt caatatgatg
     5581 aaaggtttct tttagggtaa tgttttctgg attaaaattc ttatgtttct caaatgtccc
     5641 ttcgggacat gggcctcggc gaatcggttt acggatacgt tcacaaccga tgaatcggta
     5701 tcctattatc aattgtccct ctgggacatc tgcaatcccg aagggatttc cgataataac
     5761 ccgccgattt atcggagagg cacagaaccc ggatgtaccc gccatgtccg gaacggacac
     5821 cggataatcc cgaagggatt tccgatagta accgccgatt tatcggctgt gacggaccgg
     5881 ggatactccc tttggaagga gccggattta ctgtttaaaa atagtcaatt taatttgaag
     5941 aagaaagcct atttttgccg ctttaactcc gactgatgga atcgctgctg aaagatctga
     6001 acgaacccca aaaaaatgct gtcatcaatt acgaagggcc atccatggtc ctggctggtg
     6061 ccggctcggg gaaaacccgc gtactgacat accgtgtggc ttacctggtt cacaaaggga
     6121 ttgatccgtt tcatatcctg gctcttacct ttaccaacaa agccgcccgg gagatgaaag
     6181 aacgtatcac aagcctggtc ggcgccgatg cccggaacgt ctggatgggg accttccatt
     6241 ccattttctc acggctcctc cggatcgacg gttaccggct gggctatact ccacagttta
     6301 ccatttacga tacggaagac tccaagcgta ctatccgcaa catagtcaag gagatggacc
     6361 tcgatccgaa agtttaccaa cccggctaca tccttcacag gatctcttcg gcaaaaaccc
     6421 ggatgatgtc ggccagggaa tatttcgacg acccggaaat taaagcagct gaccaatcct
     6481 ccggcaaacc gcacatcggg cagatcttct taagctatgc cgaaaggctc aaaaaatctg
     6541 acgccatgga tttcgatgac ctgctggtca atatgaatat tctgctggag cattaccctg
     6601 aggtccttct caagtatcag cgtaaattcg aatacattct cgtggatgaa taccaggaca
     6661 ccaactactg ccaatacctg attcttaaac atctagcagc taacaacgaa aatatttgcg
     6721 tggttggcga cgacgcgcag agcatatacg ctttccgggg cgccagtata caaaatatcc
     6781 tgaatttcag gcatgactat cccgatcaca ggattttcaa actggagcag aattaccggt
     6841 cgacccagaa tatcgtcaat gcagctaaca gcatcatcat aaataataaa gaccagatag
     6901 aaaaaaagat ctggacacag aatgatactg ggccgaagat caggttaata agggccaaaa
     6961 gcgacacaga agaagggacg ctcgtagtca atgccatctt cgagctaaag atgaacaaac
     7021 agctcggaaa cgacgctttt gccgttcttt accgcacgaa tgcccagtcg aggtcgttcg
     7081 aggaatcgct gcggaaactc aatattccct accggatcta tggcagtgtc tctttctatc
     7141 agcgcaagga aatcaaagat ctcctggctt atttccgtct ggcaatcaac aaccgggatg
     7201 aagacgcgct gatccgggtc attaactacc cggcccgggg gatcgggcaa acgacaatgg
     7261 aaaagatcat tgtcactgcc ggacagcatc aggtcagttc ctggcaggtc atcgaaaatc
     7321 ccgattacta tcagcttgct gtcaattctt ccacccggca caagctggaa gaatttgtcg
     7381 taatgatcaa aagtttttct gcccggatga aagccaaaga cgcctatgat cttgcccgga
     7441 cggtggctac aacatcggga attctgaaag atctttatga agatcagacg ccggaagggg
     7501 tcagccgttt tgagaatgtt gaagaattgc tcaacgctat ccgggaattt tgcgatacgc
     7561 ccagacctga accggtaatg atagaggatg acacgcccct gcccgacctc ccggccttcc
     7621 gcaccctgga tgaatttatg caggatgtgg ccctgatcac cgacgccgat gataaagatc
     7681 cgtcggaccg taaccatgtc tcactgatga cgatccatgc cgcaaaaggg cttgaatttc
     7741 cccatatttt catttcgggt cttgaagaaa atcttttccc atccatacag tcgcttaatt
     7801 cccgcgctga tcttgaagag gaacggcggt tattttatgt cgcccttacc cgcggaatgc
     7861 atagtgtaac agtatcttat gccgaaaacc gttaccggtg ggggaacctg gaaccttgcg
     7921 atcccagccg ctttattgat gaaatcgatc ccaaatacat cgaaattcca cggaagactt
     7981 ccatgtatga cctgccggtc gatttccgga aaatacgcgg agccgactcg cgggaaactg
     8041 cccccgggaa aaccgatccc cccaggaaac tgaagaaact gacaaacttt accaattact
     8101 cttccggaga ttccggaaag caggatgaac tttccgggct gcagcccgga atgatggtcg
     8161 aacatgaaaa attcggcaaa ggaaaagttc aaagtgtgga aggagaaggc agtaataaaa
     8221 aagcagttat cttctttcaa cttatcggtc agaaacaact tctgttaaaa tttgctaaac
     8281 ttaaaatcct ttgatcaaat aatttagtaa ctttgccgtt ttaaatccga cttcagacat
     8341 tgcgcggtta ataccctttc atatataaac tccggaaata tcaggcacta tcaccggtcg
     8401 gtcagtcgga aaagctcaaa aatcaaattt acatggaata taacacacag aaaaacaagc
     8461 tgatcattgc tgaatacggg cggaatctgc agcaaatggc catggactgc gccaatattg
     8521 aagaccgtga aaaacggacc cgcattgccg aattcatcgt taatgtcatg gcgcaaatga
     8581 atcccaaagt gaaagagtcg ggcgagtaca aacagaaact gtgggatcat ctttatataa
     8641 tgtccgattt caaactcgac gtcgattccc cttatcctcc cccatcacgt gaggtacttc
     8701 ttttcgccca taaatcgatt aaataccagc aaagcaatat tcgttaccgt cactacggta
     8761 aaaatattga aatgatcata gataaagcgg tgacgtatga tgaaggtcct gagaaagaag
     8821 ctcttattaa aactattgcg aatcatctga agaaatccta tcttaactgg aacaggaatt
     8881 cggtcgatga tgaagtaatt ctcaagaatc tgggcgaact ctccggtaac cgacttaagc
     8941 tgtcggaaga gcacaaactc agccacacca acgaaatcct cgcacgcaac attgttaaaa
     9001 agaagaaatt cgtcaaacca ggaaccggga accagttcgg ggcgccgggc aaccagggcc
     9061 aaccatacca taaactgaaa aagaaacagg gatgagttcg tttgaaattc acggcggaaa
     9121 aagattaaac gggaccatca ggccgcaggg ggccaagaat gaagcattac agatcctttg
     9181 cgccgtcctg ctgacccccg aaaaggtcac agtcagcaat gtcccagata tccgcgatgt
     9241 gaattttctg atcgacctgc tccgcgatat gggagtcagt gtcaataaaa cggctccgga
     9301 aaccttcgag ttccaggccg atcagatcaa cctggagtac ctgcgtacac cccagttcaa
     9361 acagaaagcg gccagcctgc gcggctcggt catgatcctt ggaccgctgc tgtcgcggtt
     9421 cagaaaaggt tacatccccc atccgggtgg cgataagata ggccgaagaa ggctcgatac
     9481 ccattttatc ggtttacaaa acctgggcgc acgctttatc ttcaatgaaa aagaaaagct
     9541 atatgaagta gaggccgaag acctcaaagg gtgctacatg ctgctcgatg aagcttcggt
     9601 caccggaacg gccaatatca tcatggctgc cgtaatggcc aaagggaaa
//
LOCUS       DAOPNH010000116         9641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7708_length_9641_cov_4.670353, whole genome shotgun sequence.
ACCESSION   DAOPNH010000116 DAOPNH010000000
VERSION     DAOPNH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_9641_cov_4.670353"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="PKZ74_07495"
     CDS             complement(<1..1854)
                     /locus_tag="PKZ74_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPM92641.1"
                     /translation="MILFYQNNHSVIYAIGSKIHQPKKDLEKLAWLLGDASLVMWEQV
                     EGQFIGPRKEMITPWSTNAVEITQNMGIEGIERIEEFRYFKEGDSFDPMLQHLYKGLD
                     QKIFTIEHKPEPILYIDDIRAYNLKEGLALSEEEVIYLEGLAEKLGRKLTDSEVYGFA
                     QVNSEHCRHKIFNGSFIIDGDVKEDSLFSMIKKTSKANPNRLVSAYKDNVAFISGPAA
                     QEFAPETQDKADFFRIRPVNTVISLKAETHNFPTTVEPFNGASTGTGGEIRDRMAGGK
                     ASIPLAGTAVYMTSYPRMAPGRPWEERLKPRKWLYQTPEEILIKASNGASDFGNKFGQ
                     PLICGSVLTFEHEEDGKSYGYDKVIMLAGGIGTGIREDSIKDIPGTGDLIVVMGGDNY
                     RIGMGGGAVSSVATGEYGNAIELNAVQRANPEMQKRVYNAIRAMAESGANPVISIHDH
                     GAGGHLNCLSELVEATGGTIDMTKLPIGDPTLSDKEIIGNESQERMGLIISEKDLPLL
                     KRIAERERAPLYVVGRTTGDHILSFVNAQGEKPMHLELANLLGKPPRTVLNDTTLPAS
                     FADPAYTVVKTQHAASPLQHAASPLQHAASLPIALHQVLQLEAVACKDWLTN"
     gene            complement(1856..2815)
                     /locus_tag="PKZ74_07500"
     CDS             complement(1856..2815)
                     /locus_tag="PKZ74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM92642.1"
                     /translation="MENREKQIVRASWVSIAGNALLSVLKIFIGLISGSMAVLADGID
                     SAGDIIASAITLVTARVISRPPNLKYVYGYERADTIASKVLSFIIFFAGAQLAITTIN
                     HLIEGEKSVMPSMLAVYVTVFSIMGKVMLAWYQYTIGKKTGSLMLQANGKNMMGDVVI
                     SLAVLIGLVFTFVMKMPVFDKITALIVSGWIIYLAFRIFRESSLQLMDGVDDESVYDR
                     VFKAISKVEGASNPHRTRIRKIGHQYMVAVDIEVDGDMPLKMAHQLAHQVEKQIKLEL
                     TDVYDVMVHTEPVGDDPAEEKFGISEDNFQKLKKTRRSKSKNT"
     gene            complement(2817..3758)
                     /gene="trxB"
                     /locus_tag="PKZ74_07505"
     CDS             complement(2817..3758)
                     /gene="trxB"
                     /locus_tag="PKZ74_07505"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPM92643.1"
                     /translation="MEHIHCLIIGSGPAGYTAAIYAARADLRPVIYEGIQPGGQLTTT
                     TEVENFPGYPDGIMGPQMMEDMKKQAARFGTDIRFGIVTEVDLSKRPFTVKADDGKTM
                     LADTLIIATGATAKYLGLESEERFMGAGVSACATCDGFFYRGKDVAIVGGGDTAAEEA
                     SYLAKLCRTVHLIHRREELRASKAMQKRVTTTPNIVLHWNCVVKEIVGTEEGFNKAVT
                     GVRIENIKTGEQELLTVDGYFSAIGHKPNSDLFKGVLDMNEVGYLITKPDSTETNVPG
                     VFACGDVQDWKFRQAVTAAGSGCMAALEAERFLAAQE"
     gene            3828..4208
                     /locus_tag="PKZ74_07510"
     CDS             3828..4208
                     /locus_tag="PKZ74_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPM92644.1"
                     /translation="MKVLIRLLVYTCAVLLTAWLLPGVSVENFTTAVMVAISLAILNT
                     FVRPILVFLTIPVTIVTLGIFLLVINALIILFIGKVIPGFTVDGFWWALLFSVILSIV
                     ASLFGTSSTTIVRRNEYEKPADNQ"
     gene            4183..4644
                     /locus_tag="PKZ74_07515"
     CDS             4183..4644
                     /locus_tag="PKZ74_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPM92645.1"
                     /translation="MKNRQITNKEHQDAIIRKCQFCHVAMTTPDGKPYVIPMNFGYDG
                     EFVYFHGSAKGKKADILKINPDVCIAFSTDHELRYVNEEVACSWSMRYKSVLVYGKAE
                     FIEDAGSKIECLNRIMAQYADRDFEYNEPAVREVMVFRVKVEKMEGRVYGY"
     gene            complement(4598..5179)
                     /locus_tag="PKZ74_07520"
     CDS             complement(4598..5179)
                     /locus_tag="PKZ74_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPM92646.1"
                     /translation="MLESLKNIDTQLFLAINGINSPAIDPVMFWISQRWIWIPLYLFF
                     AWLLYRHYGKKAVLLVILAGLMIVISDQTSVHLFKNVFLRLRPCHEPSLAGMVHLVDG
                     RCGGKYGFISSHAVNSFALATYLSFLLGKRIRYFTPLILFWAFLLSYSRIYLGVHYPG
                     DVIAGGLWGAGIGAAIFIISIRIPFLPSSQPSP"
     gene            complement(5192..6193)
                     /gene="obgE"
                     /locus_tag="PKZ74_07525"
     CDS             complement(5192..6193)
                     /gene="obgE"
                     /locus_tag="PKZ74_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPM92647.1"
                     /translation="MSSPNFVDYIKINCTSGKGGAGSAHLHRAKYIPKGGPDGGDGGR
                     GGHVILKGNRHLWTLLHLKFTKHVRAGNGGVGGKSLSTGAAGEDRIIEVPLGTVAKMA
                     DTGEVLFEITEHEETKVLLKGGRGGLGNDHFKSSTFQTPRFAQPGEPGQEAWIILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLTPNLGIVSYRDGASFVMADIP
                     GIIEGASEGKGLGLRFLRHIERNSLLLFLIPADSKDIRREFEILLNELDKYNPELVDK
                     PRLLAISKSDLLDSELISEMKKELPPVKTVFISSVTGDGILKLKDLIWTELNRYSDE"
     gene            complement(6324..6857)
                     /locus_tag="PKZ74_07530"
     CDS             complement(6324..6857)
                     /locus_tag="PKZ74_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPM92648.1"
                     /translation="MKTITVKDKTFKLSITADEISQAIDRLAAELNRDLEGRNPLFLV
                     VLNGAFIFAADLYRKITIESEISFVKLASYSGTSSTSDVKELIGLNERLKGRNIVILE
                     DIIDTGTTMQYLINKLYYLGVNDVKLAALLFKPGAFRESFRIDYLGMEIPNEFIVGYG
                     LDYDGFGRNYADIYKIC"
     gene            6975..7484
                     /gene="purE"
                     /locus_tag="PKZ74_07535"
     CDS             6975..7484
                     /gene="purE"
                     /locus_tag="PKZ74_07535"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPM92649.1"
                     /translation="MTPLVSIIMGSTSDLPVMEDAAKVLNELKIPFEMNALSAHRTPD
                     KVEEFAKSASGRGIKVIIAGAGMAAHLPGVIAAMTPLPVIGVPIKGSLEGLDSLLAIV
                     QMPPGIPVATVAINGARNAGILAAQIIATGDPALADKMLKFKEELKEKIIKANEDLSK
                     VKFEYKVGG"
     gene            complement(7491..8756)
                     /locus_tag="PKZ74_07540"
     CDS             complement(7491..8756)
                     /locus_tag="PKZ74_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92650.1"
                     /translation="MFGVPISKTTLAIIFTIVLFNKTCRSQDYYEEIVYPDSINIISM
                     LPLNFDTIFIGGSTQYATGGVYRSFDSGITWEYVGLNGLSIYCMSHGNGDTIYAGTIG
                     VYRSNNLGESWERISPIEQNVISISGFFPDDIFIGYWGGIMRSLDNGNTWDTSLVLSQ
                     NTVINTILPVGENEIYAGGTAYTAQDAGVFKSADLGEIWNYIGLVGYNIQSLGFNSNQ
                     DLFAGCFYSGLLKTSNGGLTWETVLPNRDAVSLVIEFDEIYVGCENQSFPNGGIYYSP
                     DNGLTWENRTYNITNNRIKDIVITPDQFLYSLSRYESTVLGPALNRSVNPVFINESNQ
                     DKNVPVQIFPNPASDFFQISINQKFLLEENIKIFIYNQNGQMLKSDSLVLLPDNSCPT
                     INIAEMTPGVYYVKLVIGKNKYLLKLVVI"
     gene            complement(8865..>9641)
                     /locus_tag="PKZ74_07545"
     CDS             complement(8865..>9641)
                     /locus_tag="PKZ74_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92651.1"
                     /translation="IQDVSGQKKVKVTAKGSYVSLDLAPDQIKEKALAEAKRDALMKA
                     GVAESIAVSDFLYTFEDNEKFQEIFQAFTSTETGGEVIVDKFTENRSVNEFNALVITV
                     EIEATVFVHDEKEDPTLNIKIEGIEDNYKNGEYLKFTVTAGTDGYLKIFNVTEDYNSL
                     LYPYLDEKVVSYSDDPKFMLKALQKTTFPVNKYFPDGYTLEITKPEKNKEFNLLIFVF
                     TRENIAFLEDGSDVKKIMEWIYSIPMDKRKVVQVGFVISR"
BASE COUNT         2392 a         2370 c         2311 g         2568 t
ORIGIN      
        1 gttggtcagc cagtctttgc aggcgacggc ttccagttgc aggacctggt gcagggctat
       61 cgggagagac gcagcatgct gtagtggaga cgcagcatgc tgtagtggag acgcagcatg
      121 ctgcgtcttt acaacagtat aggcaggatc cgcaaacgat gccggcaggg ttgtatcgtt
      181 gagcaccgtc ctcggtggtt ttccgaggag gttggccagt tcgaggtgca taggcttttc
      241 gccttgtgca ttgacgaagg ataagatatg gtcgccggta gtcctgccga cgacgtacag
      301 gggagcgcgt tctctttctg cgatcctttt cagcagcggt aggtcttttt cgctgatgat
      361 cagccccatc cgttcctgtg attcattgcc gatgatctct ttatcggaca gagtaggatc
      421 gccgatgggg agctttgtca tatcgatggt tccccctgtt gcctccacca gttccgacag
      481 gcagttcagg tggcctcccg cgccatggtc gtggatggag atgaccgggt tggcgcccga
      541 ttcagccatt gcccggatcg cgttgtagac ccgtttctgc atctcgggat tggcgcgctg
      601 aacggcattc agttcgatag cgttgccgta ttcaccggta gccactgacg aaaccgcgcc
      661 gcctcccata ccgatccggt agttatcacc gcccatgacg acgatcaggt cgccggtgcc
      721 cggaatgtct ttaatactgt cttcgcggat tcccgtgcca atgccgccgg cgagcatgat
      781 taccttgtcg tagccgtaag atttgccgtc ttcttcgtgc tcaaaggtga gcacacttcc
      841 gcagatcagc ggttgtccga atttgttgcc gaaatcgctg gcgccgttgg atgctttgat
      901 caggatctct tcgggggtct ggtagagcca cttcctgggt ttaaggcgct cctcccaggg
      961 tctgccgggc gccatcctgg gatacgaagt catataaacg gcggtgccgg ccagcgggat
     1021 actggctttg ccaccggcca tccggtcgcg gatctctccg ccggtaccgg tggaagcgcc
     1081 gttaaacggc tcgacggtcg tcgggaaatt atgcgtttcg gctttcagcg aaatcactgt
     1141 gttgaccggc ctgatgcgga aaaaatcggc cttgtcctgc gtctccgggg caaattcctg
     1201 tgctgccggt ccgctgataa aggctacatt atctttataa gctgagacca ggcggttggg
     1261 attcgccttc gaggttttct ttatcatcga aaaaagggaa tcctccttta catcgccatc
     1321 aataataaaa ctcccgttaa agatcttatg cctgcaatgc tctgaattaa cctgtgcaaa
     1381 tccgtatact tcgctgtcgg tcagctttct gcccaatttt tctgccagcc cttccaggta
     1441 aatgacctct tcttcgctca gggcaagccc ttccttcaga ttataagccc tgatatcgtc
     1501 gatatatagt atcggttcgg gcttatgctc gatcgtaaaa atcttctgat ccaaaccttt
     1561 gtaaagatgc tgaagcatgg ggtcgaaaga gtcgccctcc ttaaaatagc ggaactcctc
     1621 gatcctttca atgccttcga tacccatatt ctgcgttatt tcaaccgcat tggtgctcca
     1681 gggagtgatc atctctttcc ggggtccgat aaactgtcct tcgacctgct cccacatgac
     1741 cagcgaggca tcccccagca accaggccag cttttcaagg tccttcttcg gttggtgaat
     1801 tttgctgccg atggcataaa tgacagagtg gttattctga taaaaaagga tcatctcagg
     1861 tatttttgga ttttgaccta cgggtctttt taagtttttg aaaattatct tcggaaatcc
     1921 cgaatttttc ttctgccgga tcatcgccca ccggttcggt gtgcaccatc acatcataaa
     1981 catcggtaag ttcgagtttt atctgctttt ccacctggtg agccaactga tgggccattt
     2041 tgagcggcat atcgccatcg acttcaatat cgacggcaac catatactgg tgccctattt
     2101 tacggatcct ggtacgatgt ggattagaag ccccttcgac tttcgatatc gctttgaata
     2161 ccctgtcgta aacgctctca tcatccacac catccattag ctgcagcgaa gattcccgga
     2221 aaattctgaa tgccaggtaa atgatccagc cgctcacgat aagagccgta atcttgtcga
     2281 aaacaggcat tttcatcacg aaagtaaaaa ccagtcctat taaaactgcc agggatatga
     2341 ccacatctcc catcatgttt tttccattag cctgaagcat caggctgccg gtttttttac
     2401 cgatggtgta ctggtaccag gcgagcataa cttttcccat gatggagaac acagtcacat
     2461 aaacagccag catcgacggc atgaccgact tttcgccttc aatgaggtgg ttgatcgtcg
     2521 tgatggcaag ctgtgccccg gcaaagaaaa tgataaaaga gagcactttg gaggcaatcg
     2581 tgtcggcccg ttcataacca tagacatact tcaggttcgg cgggcgggaa atgacccggg
     2641 ctgtgaccag ggtgatagct gatgcgatga tatcgccggc cgaatcgatc ccatccgcca
     2701 aaacggccat ggatcctgag atcaacccta taaagatttt aagcaccgac aggagagcgt
     2761 ttccggcgat gctgacccag gaggcacgga cgatctgctt ctcccggttt tccatcttat
     2821 tcctgtgctg cgaggaacct ttcagcctca agagcagcca tacagccgct ccctgccgcc
     2881 gtcaccgcct ggcggaattt ccagtcctgg acatcgccgc aggcaaaaac acccggaaca
     2941 ttcgtttcgg ttgaatcggg tttggtaatg agataaccca cctcattcat atccaatacc
     3001 cctttaaaca ggtcggaatt cggtttgtgg ccgatagcgg aaaaataacc atcaaccgtt
     3061 agcagttcct gctccccggt tttgatattt tcaatgcgaa cgccggtcac cgctttgtta
     3121 aaaccttctt cggtgccgac aatttctttc acaacacaat tccagtgaag gacgatattc
     3181 ggcgtggtgg tcactcgctt ctgcatggct ttcgaagcgc gcaactcttc gcgtcggtgg
     3241 atcagatgca ccgtgcggca gagtttggcc aggtaggagg cctcttccgc tgccgtatcg
     3301 ccgccgccga caattgccac atcttttccg cggtagaaga acccgtcgca ggtagcacag
     3361 gccgaaacgc ccgcccccat gaaccgctct tccgattcca ggccgaggta tttggccgtg
     3421 gccccggtgg caatgatcag ggtatcggcc agcatcgttt tgccatcatc ggctttcact
     3481 gtaaaaggcc gtttcgacag gtcaacctca gtgacaatgc caaaacggat atcggtgccg
     3541 aaacgggcgg cctgcttttt catgtcttcc atcatctgcg gacccatgat cccgtccgga
     3601 taaccgggga agttttccac ttccgtggtt gtcgtcaatt gtccacccgg ctgtattcct
     3661 tcataaatta cgggacgcag atcggccctg gcggcataaa tagctgcggt atatccggca
     3721 ggcccggacc caatgatcag gcaatgaata tgttccatgt tttcgttaat cttatttggt
     3781 tttgaaagtt gaggatgcaa aggtagtaaa aagtatatct ttgtaagatg aaagtactga
     3841 tccgactctt agtttacaca tgcgccgtat tgctgaccgc ctggctgctg cccggagtca
     3901 gtgttgaaaa cttcactacg gccgtaatgg tagccatttc gctggctatc ctgaacacat
     3961 ttgtcaggcc gatactggta tttctgacga tcccggtaac catcgtcacc ctggggatct
     4021 tcctgctggt gatcaacgcg ctgatcatcc tgttcatcgg gaaggtcatt cccggattta
     4081 cggtcgacgg gttctggtgg gccctgctgt tcagcgtcat tttgagtatt gttgcttcct
     4141 tgttcggaac aagttcaacc acgattgtca ggcgaaacga atatgaaaaa ccggcagata
     4201 accaataaag agcaccagga tgcgatcatc cggaaatgcc agttctgtca tgtggccatg
     4261 accacgccgg atgggaaacc gtatgtcatt ccgatgaatt tcggctacga cggtgagttt
     4321 gtctatttcc acggctcggc aaaagggaag aaagcggata tactgaagat taacccggat
     4381 gtgtgtatcg ctttcagcac cgatcatgag ttacggtatg ttaatgaaga agtggcctgc
     4441 agctggagca tgcgctacaa gagtgtcctg gtatacggaa aggctgaatt tattgaggat
     4501 gccggcagta aaatagagtg tttgaacagg atcatggcgc aatacgctga ccgggatttt
     4561 gaatacaacg aaccggcggt gagagaggtt atggttttca gggtgaaggt tgagaagatg
     4621 gaaggaaggg tatacggata ctaattataa atattgcagc gccgatgccg gctccccaga
     4681 ggccgccggc aatcacatcg cccggataat gcacccccag gtatatccgg ctgtagctca
     4741 gtagaaaagc ccagaataag atcagcggcg taaagtaccg gattcttttt cccagtaaaa
     4801 acgacaggta agtagccagg gcaaaggagt ttacggcatg ggaagagatg aaaccgtact
     4861 tccctccgca gcgcccgtcg accaggtgta ccattcctgc cagcgatggc tcgtggcagg
     4921 gcctgagcct caggaaaaca ttcttaaaaa ggtgaacgga ggtctgatcg ctgatcacta
     4981 tcatcagacc ggcgaggatc accagtagca ccgccttttt cccgtaatgc cggtaaagca
     5041 gccaggcgaa aaacagataa agaggaatcc aaatccaccg ctgggagatc cagaacatga
     5101 ccggatcgat cgccggggaa ttgatcccgt tgatcgccag gaacaattgg gtgtcgatat
     5161 ttttcaggga ttccagcatg cttgtgcggc attactcatc ggagtagcgg ttcagctcgg
     5221 tccagatgag gtctttcagt tttaagatgc catctccggt aacggacgaa ataaaaaccg
     5281 tctttacagg cggcagctcc tttttcatct ctgatatcag ttccgaatcg aggagatcgg
     5341 atttggatat ggccagcagc ctcggtttgt cgaccagttc ggggttatac ttatccagct
     5401 cgttgagcag aatttcaaat tcccggcgaa tatccttcga atcggcaggg atgaggaaca
     5461 acagcaggga attccgttcg atatgccgga ggaaacgcag ccccagccct ttgccttcgc
     5521 tggcgccctc gataatgccg gggatatcgg ccatgacaaa cgaggcgccg tcgcggtaag
     5581 aaacgatccc gagattgggc gtcagggtgg taaaggggta gtcggcgatt tcgggtttag
     5641 cggccgagac gaccgatagc agcgttgatt tcccggcatt gggaaagccc acgaggccga
     5701 cgtcggcaag tatcttcagt tccaggatga tccaggcctc ctgtcctggc tcgccgggct
     5761 gggcgaagcg gggcgtctgg aaggtggacg atttgaaatg gtcgttgccc agtccaccac
     5821 gccctccttt cagcagaacc tttgtctctt cgtgttcggt gatctcaaac aatacctcac
     5881 cggtatcggc cattttagcg actgtgccca ggggcacctc gatgatcctg tcttcgccgg
     5941 cagctccggt gctgagcgat ttgcccccca ccccgccatt acccgccctg acgtgcttgg
     6001 tgaatttgag gtgcaggagg gtccagagat ggcggttccc tttgaggatg acatgccctc
     6061 ccctgccacc gtcgccgccg tcagggcccc ctttggggat atacttggcg cggtgcagat
     6121 gggccgaacc ggcgccccct tttccggagg tgcagttgat ctttatataa tcgacaaaat
     6181 tgggtgagga cattataaaa cgatttttta caaatgtaac tatctttttt tcaccacaat
     6241 aggaacatag gacacatagt attttcaatt agctctatgt gcctcctatt gtgtcctatg
     6301 tgtctgttgt ggtttatggt tatttaacat attttataaa tatcggcata attcctcccg
     6361 aatccgtcat aatccagccc atacccgaca atgaactcat tcggtatctc cattccaagg
     6421 taatcgatcc ggaatgattc acggaaggcg ccgggtttga agagaagggc tgccagtttc
     6481 acatcattca cccccaggta ataaagcttg ttgatgagat actgcatggt tgtccccgta
     6541 tcgataatat cttccaggat aacgatattc cgtcctttga gcctttcatt cagcccgatc
     6601 agctccttaa cgtcggaagt ggaggatgtg ccggaatagg aagccagttt gacaaaactt
     6661 atttcacttt cgattgttat cttacggtag aggtcggcgg cgaagataaa agcgccattc
     6721 aggaccacga ggaataaggg atttcggcct tccagatcgc ggtttagctc tgctgccagc
     6781 cggtcgatgg cctggctgat ctcatcggct gtgatcgaaa gcttaaaagt tttatctttg
     6841 accgtgattg ttttcattgg gttggtttag ggggacgaat actgtttcgc aaagttacgc
     6901 aaaggaaacg caaaaaatag ttcaaagttc aaagttcaaa gttcaaaatt taaaattcaa
     6961 aatttaaaat ttctatgact cctttagtta gcatcatcat gggttcgacg agcgatcttc
     7021 cggtgatgga agatgccgcc aaggttctta acgaactgaa gataccgttt gaaatgaatg
     7081 ctttgtcggc ccaccggacg cccgacaagg tggaagagtt tgccaaatcg gcttctggca
     7141 gggggatcaa agtgatcatt gccggggcgg ggatggcggc ccatcttccc ggggttatcg
     7201 ctgccatgac cccattgccg gtgatcggcg ttccgatcaa aggttcgctc gaagggctcg
     7261 attcgctcct ggccatcgtc cagatgcctc ccgggatacc cgtggctacg gttgccatca
     7321 acggagcgcg caacgccggc atcctggccg cacagatcat cgctaccggc gatccggcgc
     7381 tggccgataa aatgctgaaa ttcaaggaag aactgaaaga aaaaatcatc aaagcaaacg
     7441 aggatctttc gaaggttaag tttgaatata aggttggtgg atagtggttt ctagattaca
     7501 accaacttta gtagatattt gttcttccct ataactaatt ttacataata aacacctggt
     7561 gtcatttcag ctatattaat tgtggggcaa gaattatctg ggagcaatac taatgaatca
     7621 cttttaagca tttgcccatt ttgattataa ataaatatct ttatattttc ctcaagtaag
     7681 aatttttgat taatactgat ttgaaaaaaa tctgatgctg gattgggaaa tatttgaaca
     7741 ggtacgtttt tatcttgatt actttcatta atgaataccg gattgactga cctattaagg
     7801 gctgggccta atacggttga ttcatatctt gataatgaat acagaaattg atcaggtgta
     7861 atgacaatat ccttaatccg attgttggta atattatatg ttcggttttc ccatgtaagt
     7921 ccattatcag gagaataata aataccccca tttggaaaac tctgattttc acatccgaca
     7981 tagatttcat caaattcgat tactaaagaa acggcatctc tattcggaag tactgtttcc
     8041 caagtcaatc ctccattaga ggtctttagt aaaccggaat aaaagcagcc ggcaaacaag
     8101 tcttgatttg aattaaaccc aagtgactga atattatatc ctaccaaccc aatatagttc
     8161 catatttctc ctaaatccgc cgacttgaac actcctgcgt cctgagccgt ataagcagta
     8221 cctccggcat aaatctcatt ctcaccaaca ggtaatatgg tatttatcac ggtattctga
     8281 ctaagaacca atgaggtatc ccatgtattt ccgttatcaa gagaacgcat gatccctccc
     8341 caataaccaa taaaaatgtc gtcgggaaag aagcccgaga tagaaattac attttgttct
     8401 ataggtgaaa tacgttccca gctctcaccc aaattatttg acctatatac cccaatagta
     8461 ccagcataaa tagtgtcacc atttccgtga gacatacaat atatagaaag cccatttaat
     8521 cctacatact cccaggtaat tccgctatca aatgatcgat aaactccacc agtggcatat
     8581 tgagtactac caccgataaa gatagtatca aagtttagcg gaagcataga aataatattt
     8641 atagaatccg gatatactat ttcttcataa taatcctgag aacggcaggt tttattaaaa
     8701 agaactattg tgaaaataat ggcaagggta gtttttgaga ttggcactcc aaacatctta
     8761 tgactttttc gcctttaaat ataataattt ttaatatatc atgaggcact atagcaaaaa
     8821 ataataataa ttttggaaga acaataagct attttatcaa ctggtcaccg gctaatcaca
     8881 aaccccacct gcaccacttt tcgcttatcc atcgggatcg aatagatcca ctccatgatc
     8941 tttttcacat cggagccatc ttcgaggaag gcgatatttt cgcgggtgaa tacaaatatc
     9001 agcaggttga actctttgtt tttctccggt ttggtgatct ccagtgtata gccatccgga
     9061 aaatatttat tgaccgggaa ggttgttttt tgcagcgctt tcagcatgaa cttcgggtca
     9121 tcgctataag agaccacttt ctcatcgagg taaggataaa gaagggaatt ataatcctcc
     9181 gttacattaa aaattttcag gtagccatcg gtgccggccg tcacggtgaa tttcaggtat
     9241 tcgccgtttt tgtaattatc ttcaattcct tctattttaa tatttaatgt cggatcttct
     9301 ttttcatcat ggacaaaaac cgttgcttcg atctccacag tgatcaccaa ggcgttgaat
     9361 tcattaacag aacggttttc cgtaaattta tcaacgatca cttcgccccc ggtctccgtc
     9421 gaagtaaatg cctggaagat ctcctggaat ttttcgttgt cctcgaatgt gtaaagaaaa
     9481 tcggagaccg cgatgctttc agctaccccg gccttcatca atgcatcacg ctttgcttcg
     9541 gccagggcct tctccttgat ctgatccggt gcaagatcca gtgatacata gctccctttt
     9601 gcggtcactt tcaccttctt ctgccccgaa acatcctgga t
//
LOCUS       DAOPNH010000117         9617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7743_length_9617_cov_7.958795, whole genome shotgun sequence.
ACCESSION   DAOPNH010000117 DAOPNH010000000
VERSION     DAOPNH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7743_length_9617_cov_7.958795"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKZ74_07550"
     CDS             complement(<1..255)
                     /locus_tag="PKZ74_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM92652.1"
                     /translation="MIYLDNSATTFPKPEEVYRFMDSFYRKNGVNPGRSGFDAAIETE
                     EVVFGTRKLLTELFNGDDPNRLTFSYNASDSLNILLQGLAE"
     gene            complement(304..1584)
                     /locus_tag="PKZ74_07555"
     CDS             complement(304..1584)
                     /locus_tag="PKZ74_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM92653.1"
                     /translation="MKEDMKSKALSKLNSNGSSRLLTQLNSCVHCGLCAESCLYYEAF
                     RDARFVPAKKVELVASVYRRYYTLTGKTLPWLTGAKELDDKMIAEMIDLLYGACTMCG
                     RCNLHCSIGLDIAHVVFTGRSMLADMNLVPDTLQATVDAAIKTGNNMAIPTHELVDTI
                     KWLEEDLRMEVNDDNALIPLDEPGKNILYTLNPREPKFFPLSISAMAKIFHVAGESWT
                     ISTKMYDVTNYGYFSGNEQEASEIAGRLHREMESLGAKRCVLAECGHGSRAFRWEAPN
                     YLGKAFDFDVLTSVELIAEYIRDGRIKLDPGRLKGKVTLHDPCNLVRGGGIFEEQRYI
                     LKHAVSDFTEMEPHGPDNLCCGGGGGQLAMGEYNERRMKIAGLKADQIRKTGAKIVCT
                     PCHNCVDQLIQTNASYKLGVKIMTLAEVVSEAMV"
     gene            complement(1586..2275)
                     /locus_tag="PKZ74_07560"
     CDS             complement(1586..2275)
                     /locus_tag="PKZ74_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92654.1"
                     /translation="MEFAWFQWMALSALAICLSVLTFHLIRLIRQGSPQDYSKSAGSI
                     SRGVRYAFTGAMSPARKASAYLHLPTYTAGLIYHSGTFLSFFLFFFIITGNFPEGWLT
                     LLIVSFLAISALCGIGIFIKRMVKQELLSLSNPDDFISNLLVTIFQVFTVIILLCNTN
                     LISEGTTVQLPGCPTAQLIYFFHFTLLMLYVPVGKLRHLLYFFAARYHLGFFYGRRGV
                     WPSGNINSKAG"
     gene            complement(2284..2601)
                     /locus_tag="PKZ74_07565"
     CDS             complement(2284..2601)
                     /locus_tag="PKZ74_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPM92655.1"
                     /translation="MPTMEIEGRVFEVDGDGFLAHPEIWDKGVAELFARYDGIDPMTE
                     NHWAVVNIIRRNFEEKGMAPMIRTICQETGLRLRDVYELFPLGPARGACRVAGLPKPD
                     GCV"
     gene            complement(2659..2910)
                     /locus_tag="PKZ74_07570"
     CDS             complement(2659..2910)
                     /locus_tag="PKZ74_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92656.1"
                     /translation="MTLICKNNQGCRLVQTDVVVPDKAQKENYIRTYCESDDNWKQCQ
                     RYIIRKALWICPDFVLPDSNLTEDEVIERYEQSKAVEKK"
     gene            complement(3026..3829)
                     /locus_tag="PKZ74_07575"
     CDS             complement(3026..3829)
                     /locus_tag="PKZ74_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM92657.1"
                     /translation="MTDFRRQFGQWGLVAGAAEGIGAAFCEELARRGMNVILVDILKS
                     EMESRAAKLESEFQVKTISKVIDLGSENAASLCMEAIRETGCRLLIYNAAYSRVKPFL
                     SAEAGELDRYIDVNARTPLSLAYLFSGWLKSEGNSGGILLMSSLAGLWGTGLVAAYSG
                     TKGFNLLLAEAISRELRHLNIRVSVCVAGATATPGYLGTHPHYGLIRPSVMKPGKVAS
                     YALKRLVKRTVIIPGMMNRMTYFLLTRIFPRSVSVRLMNRTMERTYRVE"
     gene            3892..5088
                     /locus_tag="PKZ74_07580"
     CDS             3892..5088
                     /locus_tag="PKZ74_07580"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HPM92658.1"
                     /translation="MGKLLFLILSIIKNFRKLKLRLTGNTEFGISSSRVVSGKAWDDF
                     CDQLKLAGASLHYPGAPTDPFQQAEGIRYLTRLTRAGLEAFVEYNDPAFPVLRQMVHE
                     TVKMGADNPDNVYLNAQISGHYEYRITGKRNTIDYIGFFTQNGNYGSTGGLAPCGKID
                     DKQLVCEPDGSFEIILSKTPKGKNWLKIENETGLVIIRQTYLDRKTEIPAELRIENLD
                     GRKAPDPVTPEKIDEGLKTAAMFVGGASLLFSRWANNFRKHSNRLPMFDPAVSNAAGG
                     DESIIYYHSHWKLGPEEALVIEVLPPSCDTWNFQLNNYWMESLDYRYFNICLNKASAK
                     YEEDGSVRLIVSPTDPGHPNWIDTCGHTEGTMCWRWYRLKEGVQPVAPGCQVVQFKDL
                     KDHEKR"
     gene            5075..6403
                     /locus_tag="PKZ74_07585"
     CDS             5075..6403
                     /locus_tag="PKZ74_07585"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM92659.1"
                     /translation="MKKDRIIKSSHYGHRPWWFRVLNRAWRSAGKAGFKPDLSKDSLI
                     RAARKSTGLTDLGKDFMDEPLDRLLWSIREEARLHPVGAFITRQRMINLLAARLRAEE
                     MFRKNHGILDRPLLPAWVILGLQRTGTTKLHRLLAADESNRVLLSWEAINPVPLEIRN
                     SKLETRNLEPETRHLERETRNPKQDKRVAIAKKSENALRLMAPGFFAIHPVEHNAPEE
                     DILLLDITFLSTTPEATMNVPSYAAWLETTDQGPAYEYMVKLLKLLQWQRPGKRWILK
                     SPHHLEFINLIDKHFDDVHFFWTHREVTECIPSFISMAAHSRVIFSDEAADGNTIARH
                     WIRKTGYMLRKGLEYRTNNQHKNQFTDIYYDGLVGNSAGILTGIYSLDGGMTPELLEK
                     FSRAEKNNPMRKYGIHEYRLEDFGVRKEDILNETVDYRNFIKNRIRDISR"
     gene            6423..7412
                     /locus_tag="PKZ74_07590"
     CDS             6423..7412
                     /locus_tag="PKZ74_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM92660.1"
                     /translation="MPKTQKSNYTSPASSTWEGIRAAFARQQKVGSPGPDDRLDGKTV
                     LVDGASSGLGFAVATQMAMLGARVIMACRSGIPEKGEEVKRRTGNKNVEMVYVDFSDM
                     DSIRNLIATLKEEYAPFDIVVSNAAMVPAQSRKTPQGLEEMFMVNYLSKFILINGLIN
                     EGCLRRKRDGVPPPRIIFVSSESHRHPAAFDWEGFGIYKTYGMQKTVEYYGYYKLLLT
                     TFARELSRRLNNKGKTEISVFALCPGPVNSGIAREAPGIFQPLMKLVFAMFFKSPKKA
                     AEPVMYLAVSKDQEGKAFDYLFKMTRKDIDEKAEDEENGKRLWKMSENIVKTL"
     gene            complement(7519..8706)
                     /locus_tag="PKZ74_07595"
     CDS             complement(7519..8706)
                     /locus_tag="PKZ74_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92661.1"
                     /translation="MERLFNLSRIKIISVPERFKRFLISEVDWSQRLIGIKGARGTGK
                     TTLLLQQLKNLSPAEAVYVSLDDIYFGENHLVHFAESFARNGGKYLFLDEVHKYPNWS
                     QEIKNIYDSLPELNIVFTSSSALEIQKGKYDLSRRALIYELPGLSFREFLMLKYEMDL
                     PAITLDQILARHEADIFNHQPGFKPYKYFAEYLQHGYYPYFAGDEVHYHARLNETINQ
                     VLESDLPAIFNINYEAIIKLKKLLFILSAIVPYKPNINKLAQQIGSTRDTLLRYLYFL
                     HHAHIIRWLSNESEGINFLNKPDKLYLENTNIEFALGKQLVKSGTIRETFFLNQLSVN
                     HKLAYPEKGDFLVDNKYTFEIGGRKKGNRQILGIEEAYIVSDEIEFGSLNKLPLWIFG
                     FLY"
     gene            complement(8940..>9617)
                     /locus_tag="PKZ74_07600"
     CDS             complement(8940..>9617)
                     /locus_tag="PKZ74_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM20 domain-containing protein"
                     /protein_id="HPM92662.1"
                     /translation="ISLLISGLLLLILSGCYDMTIRLEKLPDNTPPTEPIYISGNFNN
                     WDPGDEDYIMQRNSDSVLEISLPKGIGEVQYKFTRGDWSTVEKDPCGFEISNRFAYYG
                     KQEVVHDTVRSWNDLPKPGCPSITIIIDTLPENTPENAVLYLASNINDWDPGSRYWMF
                     IKGPDGKYYIKVPLPNSMESEYKVTRGDWSKVEVDTDGNDIDNRSFSGNAGDEIRIKI
                     ARWKDRE"
BASE COUNT         2569 a         2356 c         2247 g         2445 t
ORIGIN      
        1 ctccgccaaa ccttgcagta atatattcag ggagtcgctg gcgttgtagc tgaatgtcag
       61 cctgttgggg tcatctccgt tgaatagttc cgtaagcagt tttcttgtcc caaacactac
      121 ctcctctgtt tcaatggccg catcaaaacc ggatctcccc ggatttaccc cgttcttcct
      181 gtaaaagctg tccataaaac gataaacctc ttccggtttt gggaatgttg ttgcagagtt
      241 gtctaagtat atcataattc caaattcctt acagtcctat tgtcctattg tcctattgtc
      301 ttactacacc atcgcttccg atactacctc ggccaatgtc attattttca ctcccaattt
      361 atagcttgca ttcgtctgta tcagttgatc cacacagtta tggcagggtg tgcagactat
      421 tttcgccccg gttttacgga tctggtctgc tttcaggccg gcgattttca tccttcgctc
      481 attgtactcg cccatggcca gctggccccc gccgccgccg caacaaagat tatccggacc
      541 atgcggttcc atctccgtaa aatcacttac agcatgtttg agaatatacc gttgttcctc
      601 aaatatccct cccccgcgaa ccaggttaca cggatcatgc aatgttactt tgcctttcag
      661 cctgcccgga tcgagtttga tcctcccatc ccggatatac tcagcgataa gttccactga
      721 agtcagcacg tcaaaatcaa atgctttgcc gagataattc ggcgcttccc agcggaaagc
      781 cctggatccg tggccacatt cggccagcac acaccttttt gctccgagag actccatctc
      841 acggtgcaac cggccggcaa tctccgacgc ttcctgttca ttgccactaa aataaccgta
      901 attggttaca tcgtacatct tcgtcgagat cgtccagctt tcgccggcta catgaaatat
      961 tttcgccatg gctgagatcg aaagcgggaa aaacttgggc tcccggggat tgagcgtata
     1021 cagaatgttt tttccgggtt catccagggg aattaaggca ttgtcgtcgt tcacttccat
     1081 ccggagatct tcttcgagcc actttatcgt atcgaccagt tcatgggtcg ggattgccat
     1141 attgttaccc gttttaatgg cggcatcgac tgtagcctgc agcgtatccg gtaccaggtt
     1201 catgtcggcc agcatcgatc gacccgtaaa aacaacatgg gcaatatcga gcccgatgga
     1261 acaatgcaga ttgcatcggc cgcacattgt gcaggcaccg tatagcagat caatcatttc
     1321 ggcgatcatt ttatcgtcga gttcctttgc gcccgtcagc cagggcaggg tcttgcccgt
     1381 cagggtataa taccggcggt ataccgatgc gacgagttct acttttttcg ccggtacgaa
     1441 ccgggcgtct ctgaaagcct catagtataa gcaactttcg gcgcataaac cgcaatgtac
     1501 acagctgttc aactgggtca gcaaccggct gcttccatta gaattcaatt tgctgagcgc
     1561 cttgctcttc atgtcttctt tcatctcaac cggctttgct gttgatatta ccggagggcc
     1621 atacacctct ccgcccgtaa aaaaatccca ggtgatacct tgcagcaaaa aagtaaagca
     1681 aatgccgcag tttcccaacc gggacgtaca gcatcaggag tgtgaagtgg aaaaagtaaa
     1741 tcagttgggc agttgggcag ccgggcagtt ggacagtcgt tccctctgat ataaggttag
     1801 tattacagag aagaataatt actgtaaata cctggaaaat agttacgaga aggtttgaaa
     1861 taaaatcatc gggatttgac aatgacaaca attcctgttt gaccatcctt ttaatgaaaa
     1921 taccgatacc acacaatgca gaaatggcca gaaaagatac gataagcaag gtcagccaac
     1981 cctccggaaa attaccggtt ataatgaaga agaacaggaa gaaagagaga aaagtaccgg
     2041 aatgatagat caagcctgcc gtgtaagtgg ggagatgaag gtatgccgag gcttttcgcg
     2101 ccgggctcat ggccccggtg aaggcgtacc ggaccccgcg ggagatgctt cctgcagact
     2161 tggaataatc ctgagggcta ccctgccgga tcaggcggat caggtgaaaa gtcaggacag
     2221 ataagcaaat cgccagggca gataatgcca tccattgaaa ccaggcaaat tccatagata
     2281 agatcaaacg cagccatccg gtttgggaag tccggcaacg cgacaggcgc cgcgggctgg
     2341 ccccaacgga aacaattcat aaacatctct taaccgcagc cccgtttcct ggcaaatcgt
     2401 ccggatcata ggagccatcc ccttctcttc gaaattcctc cggataatat tcaccacagc
     2461 ccagtggttc tctgtcatcg gatcaatccc gtcgtaccgg gcaaatagct cggcaacccc
     2521 tttgtcccag atctccgggt gggccagaaa accatccccg tcgacctcaa agacccgtcc
     2581 ttcaatttcc atcgttggca tactttagaa ttttgagttt tgagtgttta atttttaatt
     2641 aatatttcgg actgaatgtt attttttttc aacggccttg ctctgttcat acctttcaat
     2701 gacttcgtct tctgtcaggt tggaatcggg caggacaaaa tcggggcaaa tccagagagc
     2761 ttttcggata atatatcgct ggcattgctt ccagttatca tctgattcac aataggttct
     2821 aatataattt tctttttggg ccttatcggg tacaacaaca tcggtttgaa ccagcctgca
     2881 tccctgattg tttttacaaa tcaaagtcat tattatcatt tatcaggagc cgaaattata
     2941 aaaaaaggtt gttcaaacga tcaattgttc aagagttcaa ttgttagaag agtaatttag
     3001 tttgagatta atttcagata ccctatcatt ctacccggta tgttctttcc atcgtccggt
     3061 tcatcagtct caccgacacg cttcttggaa atatcctggt gagaagaaaa taagtcatcc
     3121 ggttcatcat cccgggaatg attacagttc ttttgaccag tcttttcaat gcatacgatg
     3181 caactttgcc gggtttcatc acagaaggcc ttataagacc ataatgcgga tgggttccca
     3241 ggtaaccggg tgtagcggta gcccccgcaa cacaaacgct cacgcggatg ttcaaatgac
     3301 ggagctccct cgaaatggct tctgctaaca gcaggttgaa tccttttgtc ccggaataag
     3361 ccgccaccag tcctgttccc cataatcccg caagcgacga catcaacagg atccctcccg
     3421 aattgccttc gcttttgagc catcctgaaa acaagtaagc aagactgagt ggcgttcttg
     3481 cattcacatc gatataccgg tcgagctcac cggcttccgc agagagaaaa ggcttgaccc
     3541 ggctgtatgc cgcattatag atcaacaggc ggcaaccggt ctccctgatg gcctccatgc
     3601 atagtgatgc agcattttcc gacccgagat cgatcacttt gctgatggtt ttgacctgaa
     3661 actccgattc aagttttgca gcacgagact ccatctcact tttcaggata tccaccagga
     3721 tcacattcat cccccttcgg gccaactcct cgcaaaaagc cgcgccaatg ccttccgcag
     3781 ccccggcaac caatccccac tgaccaaatt gtcgacgaaa gtcagtcatg attatttttt
     3841 atatttgcgc accactatag tgccgtgcaa tgataacaaa ttctgatcag aatggggaaa
     3901 ttgctcttct taattttatc gatcataaag aatttcagga agttaaagct gaggttaacc
     3961 ggtaatacag aattcggtat ttcctcatcg cgtgtggtct caggaaaagc ctgggatgat
     4021 ttctgcgatc agctcaagct tgcaggggca tccctccact atcccggagc acccaccgac
     4081 cctttccagc aagcggaagg tattcgctac ctgacccgcc ttacccgagc cggcctcgaa
     4141 gcgttcgtcg aatacaacga ccccgccttc ccggtcctcc gccaaatggt gcatgaaacg
     4201 gttaaaatgg gtgccgataa tcccgacaat gtctatctca atgcccagat cagcggacat
     4261 tacgaatacc ggatcaccgg gaaacgaaac accatcgatt atatcggttt tttcacccaa
     4321 aacgggaatt acggctcaac cggcggactg gccccctgcg gaaaaatcga tgacaagcaa
     4381 cttgtttgcg agcccgacgg cagcttcgag atcatcctca gtaaaacgcc aaaggggaaa
     4441 aactggctta aaattgaaaa tgagacagga ctggtcatca taaggcaaac ttatctggac
     4501 cggaaaacgg aaattcccgc cgaactccgg atcgaaaacc ttgacggcag aaaagcgccg
     4561 gacccggtta cgcccgaaaa gatcgacgaa gggctgaaga cagccgccat gttcgtcggg
     4621 ggcgcttcgt tgctgttcag ccggtgggcc aataacttcc ggaaacactc caacaggctg
     4681 ccgatgttcg atcccgccgt ttccaatgcc gccggtggcg atgagagcat catctattat
     4741 catagtcact ggaaactggg cccggaagaa gcgctcgtca tcgaagtgct gccgccttcc
     4801 tgcgacacct ggaacttcca gttgaataat tactggatgg aatcacttga ttaccggtat
     4861 tttaatattt gtctgaataa agcatcggca aaatatgaag aggatggatc ggtcaggctg
     4921 atcgtttcgc ccaccgatcc cggccatccg aactggatcg atacctgcgg ccacaccgaa
     4981 gggacgatgt gctggcgctg gtaccgcctg aaagaagggg ttcagccggt agcgcccggt
     5041 tgccaggttg tacaattcaa ggatttgaaa gaccatgaaa aaagatagga tcatcaaatc
     5101 gtcgcactac ggacacaggc cctggtggtt cagggtattg aaccgggcct ggagatcggc
     5161 cggcaaagcg ggcttcaaac cggacctcag caaagacagc ctcatcagag cggcaagaaa
     5221 aagtaccggt ctgaccgacc tgggcaaaga ttttatggat gaaccgctcg accgtttgtt
     5281 gtggtcgatc cgggaagaag cacggctcca tcccgtcggt gcgttcatta cccggcaacg
     5341 gatgatcaac ttgctggccg cccgactcag ggctgaagag atgttcagga aaaatcacgg
     5401 catcctcgac cggcctttgt tgccagcatg ggtcatcctg ggactccaaa ggacaggcac
     5461 gacaaaactt catcgtcttc ttgcagccga tgaaagcaac agggtccttt taagctggga
     5521 agcgataaac ccggtaccat tagaaattcg aaattcgaaa ctcgaaaccc ggaacctgga
     5581 acctgaaact cgacacctgg aacgcgaaac ccggaacccg aaacaggata aacgtgttgc
     5641 tattgccaaa aaaagtgaaa atgctttgcg gttaatggcg ccgggttttt ttgcgatcca
     5701 cccggtggag cataacgcac cggaagagga tatcctgctg ctcgacatca ctttcctgag
     5761 cacaacgccg gaggcgacca tgaatgtgcc gagctacgcc gcctggctcg aaacgaccga
     5821 ccagggcccg gcgtatgagt acatggtcaa attgctaaaa ctgctgcaat ggcaaaggcc
     5881 gggcaagcga tggatcctga aatcgccaca tcatcttgag tttatcaatc tgatcgataa
     5941 acatttcgat gatgttcatt tcttctggac ccaccgggaa gtgaccgaat gcatcccgtc
     6001 gtttatcagc atggcagcgc acagccgggt tattttcagc gacgaggctg ccgacggaaa
     6061 taccattgcc cgccattgga tcagaaaaac agggtatatg ttgcgaaaag gattggaata
     6121 caggacaaat aaccagcata agaatcaatt caccgatatc tattatgacg gcctggtcgg
     6181 caactctgcc gggatcctta caggtattta cagcctcgat ggcgggatga caccggaact
     6241 gctggaaaaa ttctcccggg cagaaaagaa caatccgatg cgcaaatacg ggatccatga
     6301 atacaggctg gaggatttcg gggtcaggaa agaggatatc ctgaatgaaa ctgttgacta
     6361 tagaaatttt attaagaatc gcattcgaga tatttctcgc tgatttataa ctaatttttt
     6421 ccatgccaaa aacacaaaaa tcgaattaca caagccccgc cagttcaacc tgggaaggga
     6481 tcagggctgc atttgcaaga cagcagaaag tcggctcgcc aggtcctgac gaccgccttg
     6541 acgggaagac tgtcctcgtt gacggggcca gctcagggct tggatttgca gtggctacgc
     6601 agatggcgat gctcggtgcc cgggtgatca tggcctgccg gagcgggatt ccggagaagg
     6661 gtgaagaggt gaaaaggaga acaggaaaca agaatgtaga gatggtctac gtggattttt
     6721 ccgatatgga ttcaattagg aatctcattg caacgctgaa ggaagaatac gccccgttcg
     6781 atatcgtcgt cagcaacgcc gcgatggtgc cggcgcagag ccgtaaaaca ccgcaggggc
     6841 tggaagagat gttcatggtg aattatcttt ccaaattcat tttaatcaat ggtttgataa
     6901 atgagggctg tctgcgtcgg aaaagggatg gcgtccctcc tccacggatc atctttgtat
     6961 catccgaaag ccaccgtcat ccggctgcat tcgactggga aggatttggc atttacaaaa
     7021 catacgggat gcaaaaaaca gtcgaatatt acggttatta caaattgctg ttgacaacgt
     7081 ttgcccgcga actttcccgg agactgaaca acaaaggcaa aaccgaaatc tccgtttttg
     7141 ccctgtgccc cggcccggta aactcaggga tcgcccggga agcgccgggc atattccagc
     7201 ctttgatgaa gcttgtcttt gccatgttct tcaaatcacc gaagaaggca gccgagccgg
     7261 tcatgtacct ggccgtatca aaagaccagg aagggaaggc gtttgattat ctttttaaaa
     7321 tgaccaggaa ggatatcgat gaaaaggctg aagatgagga gaatgggaag aggctttgga
     7381 aaatgtcgga gaatatcgtt aagacgcttt agaccggaaa gatccggtgg catccgggaa
     7441 gtggaaagcg ggccggtgaa ttattcgaac aggaaattta ggaattggta aggctctatt
     7501 cgaagaccga cttcggcttt agtacaaaaa tccaaaaatc catagcggca gtttattcag
     7561 tgagccgaat tcgatctcat cagaaacaat atatgcctct tcaatgccca atatctgtct
     7621 attacctttt ttccggccac ctatttcaaa ggtatatttg ttgtcgacca ggaaatcgcc
     7681 cttttcggga taagccagtt tatggtttac cgataattgg ttcaggaaaa aagtttctct
     7741 tattgtcccg gattttacca gttgtttccc cagagcaaat tctatattag tgttttcaag
     7801 ataaagtttg tcaggcttat tgagaaaatt aatgccttca gattcatttg ataaccagcg
     7861 gataatatgt gcatgatgta gaaaataaag gtagcgaagc aaggtatctc ttgtcgagcc
     7921 gatttgttgt gccagtttgt ttatattcgg cttatagggc actatggcag acaggatgaa
     7981 aagtagtttt ttaagcttga tgatcgcctc ataattaatg ttgaagattg caggtaaatc
     8041 actttcgaga acctggttta tggtttcatt caggcgggca tggtagtgaa cttcatctcc
     8101 ggcgaaataa ggatagtagc catgctgcag gtattcagca aaatatttat atggtttgaa
     8161 tcccggctgg tgattaaata tgtccgcttc atgtctggca aggatctgat caagcgtaat
     8221 tgccggaaga tccatttcat atttgagcat cagaaattcc ctgaaggata gtcctggaag
     8281 ctcatagatc agtgcacgcc ggctcagatc atatttgccc ttttggattt ccaatgctga
     8341 tgaacttgta aaaacgatat tcagttccgg aagagaatca tagatatttt taatttcctg
     8401 cgaccagttc gggtacttat ggacctcatc caggaacaag tattttccgc cgttcctcgc
     8461 aaagctctcc gcgaagtgaa cgaggtggtt ttccccaaaa taaatgtcat cgaggcttac
     8521 ataaactgct tctgcagggg atagattttt aagttgctga agaagcaggg ttgtttttcc
     8581 tgttcctctt gcccctttaa ttccgataag gcgttgtgac caatccactt cgctgatcag
     8641 gaaacgtttg aacctttccg gcacagatat tattttgatg cgggataaat tgaacagtct
     8701 ttccataaaa aagcttttag aaaggacaaa tataatataa taaacactta ttgagaagtg
     8761 aattcaatat tttgaattaa cgtttctatt gactctccgg atggcaggag agttccgtgg
     8821 aaggacgctg ggtatataat ccaacactta tcaatgcata aaatgcaatc acgattaatt
     8881 tttttgacat ggcgtattag gattgatcaa agtcctaaat atacgaaata tatgatcccc
     8941 tactcccggt ctttccacct ggcaattttg atccggatct catcccccgc attgccgctg
     9001 aacgaacggt tgtcaatatc attaccgtcg gtatcgactt ccactttact ccaatctccg
     9061 cgggtgactt tatattccga ttccatgctg tttggcagtg gcacttttat ataatacttg
     9121 ccgtcaggtc ctttaatgaa catccagtaa cgacttcccg gatcccagtc gttgatgtta
     9181 gaggcaaggt aaagaacggc attttcagga gtgttttcag gaagagtatc aataattatg
     9241 gtaatgctcg gacagccggg ttttggcagg tcattccagc tccttaccgt atcgtggacc
     9301 acctcctgtt ttccatagta ggcgaaacgg tttgagatct cgaaaccgca cgggtctttt
     9361 tccactgtgc tccagtcgcc ccgggtgaac ttgtactgaa cctcgccaat ccctttgggg
     9421 agactgatct ccaggaccga gtcgctgttt ctttgcatga tataatcctc atcacccggg
     9481 tcccagttgt taaaattgcc ggaaatgtat ataggttctg tcggaggcgt attatccggc
     9541 aacttctcca gccggatcgt catatcataa caacccgaca ggatcagcag cagcaatcct
     9601 gaaatcaaaa ggcttat
//
LOCUS       DAOPNH010000118         9606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7759_length_9606_cov_5.190975, whole genome shotgun sequence.
ACCESSION   DAOPNH010000118 DAOPNH010000000
VERSION     DAOPNH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7759_length_9606_cov_5.190975"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="PKZ74_07605"
     CDS             complement(<1..2157)
                     /locus_tag="PKZ74_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPM92663.1"
                     /translation="MTVKTEIFKVEGMSCTACATSVESALSSASGVKRANVNFAANTV
                     AVTYDERTTGFEKLKKTLLASGYDLAPDPAGDAEMIAADEKSRLKNIRNRTIGSAVLS
                     LPVVVVSMAFHAHDRFHILLFVLTLPVVAWFGREFFIHAFKRAIHLTANMDTLVAIGT
                     GTAFIFSAFNTFFPDYLASRGLEAHVYYEAAAVIITLILLGRYFEERAKFRTSGAIRK
                     LMGLAVKSAVVIREGVESEIPVDQVIPGDLILVRPGDKIPVDGEVVSGNSTVDESMIT
                     GEPVPVKKKAGDTVIGATINNSGAFRMVARKVGEEMMLARIIRKVREAQGSKAPVQKL
                     ADRIAAVFVPVVLAISIITFVIWWFFGPEPSLTYAFITSITVLIISCPCALGLATPTA
                     IMVGIGRGAESGILIKDARSLEIANRLDVVVLDKTGTVTEGQAKITGWIWVNEEVDKE
                     GTKEMILAAEKMSEHPVARAIAHQLAEEGVKEAPLEDFMNIPGKGIIAKSNGKQCLLG
                     NRKLMDSKDIPIPGHVTDRINLWTGEGITVNYIAIEGKAICAVGVNDPVRPDSVAAIK
                     ALQDAGKEVHMITGDHPGSAERVARAAGIMHYRAQVTPEGKLDYVKELQSRGLKVAMT
                     GDGINDAPALAQADVGIAMGTGTDVAMETAEITLLKGNLMKIAEAIVLSGKTMRIIRQ
                     NLFWAFFYNIIGIPVAAGILFPFTGLLLDPMIAGAAM"
     gene            complement(2160..2369)
                     /locus_tag="PKZ74_07610"
     CDS             complement(2160..2369)
                     /locus_tag="PKZ74_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPM92664.1"
                     /translation="MAILKFKTTIKCNGCIKAVTPGLDTISDIKSWKVDLNSPDRILT
                     VEGDNPDAGRIMEALKNAGYKSELI"
     gene            2571..2858
                     /locus_tag="PKZ74_07615"
     CDS             2571..2858
                     /locus_tag="PKZ74_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92665.1"
                     /translation="MTDNIHKKSDKRDNVNGFEEILHGSTFIYGFATVTNSFQKLAGD
                     FSAASYGLMNRNSLLFSSFIFFGCMKECTASSSGEIIHGAPAGYPFFFRIP"
     gene            3199..6477
                     /locus_tag="PKZ74_07620"
     CDS             3199..6477
                     /locus_tag="PKZ74_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM92666.1"
                     /translation="MKKRIFLLFLLAVTAVSLVSAQGRVITGNVTEADGNPIPGVTVY
                     EKGTTNGTITDMNGKYQLTVPANATTLIFSFVGMQTQEIEIGGRAMIDVIMESSTVSL
                     GEVVVTAIGISRDKKALGYAVQEVSGQSVSDSRNTNVINSLSGKVAGVTVTSSSGMAG
                     ASTFITMRGFNSITQENQPLFVIDGVPVDNSMDYSGNPDDGINNLGKGVGYSNRMIDL
                     NPDDIQNVSVLKGGAATALYGLRAANGVVLITTKSGKVKGDSKVNVSFNSSVSFDQVN
                     KLPGLQNSYAQGRYGEWLGPETFNRYSWGPKYDTLYWDNNIQDDGVTPYDEPYMFDKN
                     GRIVGASAANAMQKATPYDNLGTFFQTGVSVNNSLSMAGGNDAASYYLSFSHSKTEGI
                     IPNNTYQKYTLKMSGDTKLGRLFTVSGSANYMKSGGNRIQQASNLSGVMLGLLRTPAS
                     FDNSNGLSDPVNNPEAYLFPDGSQRSFRWGIYDNPYWTVNRNEFNDDVNRIISQATVT
                     CAPTSWLNITYRLGNDFYIDRRKGTLAINSAEQPTGQILEDHHTNWDFNSDLIFNFKK
                     DIKDISLNFNVGHNIFQHYHQQIYVQGDNLTIPDYYNMANASSVITRETITQKRTAAF
                     YGDFGFGYKSMLYLNLTGRNEWSTTLPEKKNSFFFPSASLGFVFTELPALRDNKVLSF
                     GKLRTSYAIVANDAPEYSTATYFISSYWIDGWTNGISFPFAGQTGFMNDDLLGNEDLK
                     PERMKSFEAGTELRFFGNRLNFDLTYFYNKNEDLIIVVPLAGSSGYTAQTMNAATMEN
                     KGIEAVLGGSPVKNDKLEWTLNVNFTKIKNTVLSLADGVESVNLGGYTGSDIRAVAGM
                     PYATIYGSQWVKDDNGNVIIDDRETIGGEPNDHYGYPFLSDEEGPLGSALPDWTCGIS
                     SLLNYKGIMFSFLFDIRTGGYMWNGTRGALYSFGMHEDTEDRGSMTVFDGIKGHPVTN
                     PDGSVTYQTSGVNDIEAIKDENWYNGLGGGFAGPIEPFIEESGWVRLREVSLAYTFDK
                     MLEGTFIKSATLVLTGKNLWLSTPYTGIDPESSTFGASNAQGIDYFNTPNTRSYIVSL
                     KLNF"
     gene            6498..7889
                     /locus_tag="PKZ74_07625"
     CDS             6498..7889
                     /locus_tag="PKZ74_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM92667.1"
                     /translation="MKRIRTNIITAIILSLAVLVTSCKKWIDPEMNDDPNKPTDVSLN
                     LLLPSIEAGIGYAVGGDLSYATLMWMQQYSGLGNQPIAFDRYNYTQSDVDNVWKWALY
                     AGVLKDLHTMADKADEENSPYYGGIARVLTVYMLGTTTDLWGDVPYSDAFQGNENLKP
                     VYDTQQQIYDTMAVMINEAIADLTAETSNFVPGSDDLIYGGDLSLWLKAAYAVKARYA
                     LHLSERDATAYQKALDALSNGITSNEEDLEFHFGTPANEENPLYQFLIQRPGDITMCA
                     NFVNMLIQDADPRLGQFCDTTGGNYGAHPGTGDGDCPPSGYYTQNDSPVPFVSYVELL
                     FIKAESEYKLGIGDPKVTVKEAVAQSLAKFGIDDPAWTAAFAAKIDALTGEELYAEIM
                     RQKYIALFNQVETFTDWRRTGYPELEPAAYAATNDGQLPRRYPYPTSERIYNGDNMPD
                     GLTLSTRVWWDLY"
     gene            complement(7886..9394)
                     /locus_tag="PKZ74_07630"
     CDS             complement(7886..9394)
                     /locus_tag="PKZ74_07630"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPM92668.1"
                     /translation="MDNAILLTKIIATLGPSSSSVELVGQLIREGVRVFRVNFSHGSI
                     DSFSRIIDNVRQAAALSGVPAAILGDLSGPKIRIGQVPDPGIELIKGEKVEFTAQDTP
                     AFRKEDGTVVFSTTSPEIVCEVRTGQRIMIDDGNVVLICTGKHEDRLVCEVMQAGRVS
                     SKKGINLPETDLTVAAMTEWDDRCVEFAVAKKVDYLALSFVRHAKDIIHLKEILAALG
                     ARPFSPFEYHADDMSGVPDDVQFIPIISKIEKPQAIDDLDGIVAESDAIMIARGDLGV
                     EMELAEVAVLQKKIISACHDQGVPVIVATQMLQSMIESPVPTRAEVSDVANAIYDGVD
                     AVMLSGETAVGRYPVETVRMMARIARLTNEHIRSRKIARDVPGQLRESKYRSAALAHG
                     VKSIVRDIDAKLIIIWSKFGGGALYLSQLDIPLPIIAFSNLPATLNRLSLLYGIQPEL
                     MEQPETPEEFIRKAGEKVLKTRRAGHGDAVIFVYGEPIRSSGVTNSIYIHYL"
     gene            complement(9411..>9606)
                     /locus_tag="PKZ74_07635"
     CDS             complement(9411..>9606)
                     /locus_tag="PKZ74_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92669.1"
                     /translation="IRYMDYFNQYNNGQWILDIRAGYALSDHNEFSLLVNNMLNNIYS
                     LRPLKAEPMRSVQLQYIHKF"
BASE COUNT         2404 a         2435 c         2354 g         2413 t
ORIGIN      
        1 catggccgcc ccggcaatca tcggatcgag gagcagcccg gtaaatggga aaagaatacc
       61 tgctgcaacg ggaatcccga taatattgta gaagaaagcc cagaaaaggt tttgacggat
      121 gatccgcatg gttttccccg aaaggacgat agcttcggct atcttcatca ggttgccttt
      181 cagcagcgta atctctgctg tttccatcgc cacatcggtc ccggtaccca tggcaatccc
      241 tacgtcggcc tgggccaggg caggagcatc attaatgccg tcgccggtca tggccacttt
      301 cagtcctcgc gattgcagct cttttacata atcgagcttt ccttcgggtg tgacctgtgc
      361 cctgtaatgc atgatcccgg cggcccgggc aactctttca gccgatcccg gatggtcgcc
      421 tgtgatcatg tggacctcct tgccggcatc ctgcaacgct ttgatggccg ccaccgaatc
      481 gggccttacc ggatcattca cgccgaccgc gcatattgct ttaccttcaa tggctatgta
      541 attcaccgtt atcccttcgc cggtccatag attgattctg tccgtaacat gaccagggat
      601 agggatatct ttactgtcca tcagcttccg gttccccagc aggcattgtt ttccgtttga
      661 ttttgcaata attcccttac cggggatgtt catgaaatcc tccagtgggg cttcttttac
      721 accttcttct gccagctggt gtgcgatagc ccgggcgaca ggatgctccg acatcttctc
      781 ggctgccagg atcatctcct tggttccttc tttgtcgacc tcttcattta cccagatcca
      841 gcctgtaatc ttcgcttggc cttccgtaac ggtgcctgtt ttatccagca cgaccacgtc
      901 gagccggttt gcgatctcga ggctccgggc gtctttgatc aaaatgcccg attcggctcc
      961 gcggcctatc ccgaccatga tggcagtagg agttgccaga cccagcgcgc acgggcagga
     1021 aatgatcaga acagtgatag atgtaatgaa agcataggtc agcgagggct caggcccgaa
     1081 gaaccaccag atgacgaaag tgatgataga gattgccagc acaaccggta caaataccgc
     1141 ggcgatcctg tcggccagct tctgtaccgg cgctttgctc ccctgtgcct ccctcacttt
     1201 gcgaatgatc cgtgccagca tcatctcttc gcccaccttt cgggcaacca tccggaatgc
     1261 ccccgaatta tttatcgtgg cgcctatgac cgtatcgccc gccttttttt tcaccggtac
     1321 cggttcgccg gtgatcatgc tctcatccac cgttgaattc ccggatacca cttctccgtc
     1381 gaccggaatt ttatcccctg gcctgaccag aatcagatca ccaggaatca cctgatcgac
     1441 gggaatttcc gattcaactc cttcccggat caccacggcc gacttgacgg ccaggcccat
     1501 cagtttgcgg atggctcccg atgtgcggaa tttagctctt tcttcgaaat atcttcccag
     1561 caaaatgagg gtgataatga cggcagccgc ttcgtaatac acatgcgcct ccaggccgcg
     1621 gctggcaagg taatcgggaa agaacgtgtt aaaagcactg aaaataaatg ccgttccggt
     1681 accgatggct accagggtgt ccatattggc ggtcaaatga atagctcttt taaaagcatg
     1741 gataaagaac tccctgccaa accaggcgac aaccggcaat gtcaatacaa aaagcaatat
     1801 atgaaaccgg tcgtgggcgt gaaatgccat cgaaacaacg acaaccggca gcgataaaac
     1861 ggctgatccg atggtcctgt tccgtatatt ctttaacctg gatttctcat cggcggcaat
     1921 catttcggca tcgccggccg gatcgggagc caggtcatat ccagaggcca gcaatgtttt
     1981 tttcagcttt tcaaatccgg tggttcgctc gtcgtatgtt acggcaacag tattcgcagc
     2041 gaaattcaca tttgcccttt tgacaccact ggcactggag agggcgcttt ctacgctggt
     2101 tgcgcatgcc gtacacgaca tgccttccac tttgaatatt tcagttttaa ccgtcaattt
     2161 cagattaatt cagatttgta accggcattt ttcaacgctt ccatgatccg gccggcatcc
     2221 ggattatccc cttcaacggt cagtatccgg tcgggagaat tcaaatccac cttccaggat
     2281 tttatgtcac ttattgtatc aagtccgggt gttacagctt tgatgcagcc gttacattta
     2341 attgttgttt tgaacttcaa tatggccata ttatccgttt ttaatatgct aaattaacga
     2401 ttaatgacct ggttttgttt tgcgaaaagt tatccggttc gggttaatgt taatattggc
     2461 aggatgttga tcataacacg atatcagtaa attggtaact gctgattatt gttttgtgat
     2521 tgatttatgg ctttttactg actaaatttg gtcaaccagc aataaatcca ttgactgata
     2581 acatccataa aaaatccgat aaaagggata atgtgaacgg atttgaggaa atccttcacg
     2641 gaagtacttt catctatggt tttgccaccg tgaccaattc ttttcaaaag ctggccggcg
     2701 atttctccgc ggccagttac gggttaatga acaggaactc gcttctattt tcctccttca
     2761 ttttttttgg ttgcatgaaa gaatgcactg cttcctcttc cggggaaatc attcatggcg
     2821 ccccggcagg ttatcccttc tttttcagaa taccttaatg tcaaactaaa agaataataa
     2881 tgaaaacatc ttacactgac gacacttcct gaaaaagtta ctgcctgaca gccagaaggt
     2941 aaaaagaatc cccgggttgc aatccgggga atcgtgaaga agcatttcca tgcaaggctt
     3001 gccaccgtga aaacatgaag atgttccggc attaaatata caacatttta tcttgttttg
     3061 tcaagagttg tatagctttt tctttcccgg ggattcatca ccttctattc ttgtccaaac
     3121 agaaaatccg gcctgaagcg gatgcacagc ttaccggaca aattatctca aaatttaaaa
     3181 accaaatcaa ttaagattat gaagaagcga atctttttac tctttctgct tgcggtgaca
     3241 gccgtttccc tggtcagcgc acaaggcagg gtcatcaccg ggaatgttac cgaagcggat
     3301 gggaatccga tacccggggt taccgtttat gaaaaaggga cgacaaacgg gaccataacc
     3361 gatatgaacg ggaaatacca gttgacagta cctgcaaatg ctactacttt gatcttcagc
     3421 tttgtcggca tgcagacgca ggagatcgaa atcggcggcc gggccatgat cgacgtgatc
     3481 atggaatcat caaccgtttc actgggagag gtggtagtga cagccatcgg catctcccgc
     3541 gacaagaaag cgctgggata tgccgtacag gaagtcagcg gccagagtgt ttccgattcc
     3601 cggaacacga atgtcatcaa ctcgctgagc ggcaaggtcg ccggcgtcac cgttaccagt
     3661 tcttccggca tggcaggggc atccaccttc atcacgatga ggggtttcaa ctccatcacg
     3721 caggaaaacc agcctctttt tgtgatcgac ggtgttccgg tcgacaattc gatggattac
     3781 tcgggaaatc ctgatgatgg gatcaacaac ctggggaagg gggtcggata ttctaaccgc
     3841 atgatcgacc tgaaccccga cgatatccag aatgtttcgg tactcaaagg tggggctgca
     3901 acagcgcttt acggcctgcg cgcggcaaac ggcgtcgttc tgatcacaac aaaaagcggt
     3961 aaagtcaaag gcgactctaa agtgaatgtc tcatttaatt cttcggtcag tttcgaccag
     4021 gtcaacaaat tacccggatt gcagaattcc tatgcgcaag ggcgctacgg cgaatggctg
     4081 gggccggaga cctttaaccg gtattcatgg gggcctaagt atgataccct ctactgggat
     4141 aacaatattc aggacgacgg ggtaacgccg tatgacgaac cgtatatgtt cgataaaaac
     4201 ggccggattg tcggcgcaag tgcggctaat gccatgcaaa aggccacgcc gtacgacaac
     4261 ctgggtacct tcttccagac aggggtgtct gtcaataatt ctctcagtat ggccggtgga
     4321 aatgatgcag catcatatta cctgtcattt tcccactcca aaacggaagg aattataccg
     4381 aacaacacct atcagaaata tactttaaaa atgtcgggcg acacgaagct gggccggctg
     4441 ttcactgtat cgggttctgc aaattacatg aaatcagggg gtaacaggat ccagcaagcg
     4501 tccaacctgt cgggtgtgat gctgggcctt ctccggacac cggcatcatt tgataattcg
     4561 aacggcttat ccgatccggt caataatccg gaggcctatt tgttccccga tggttcgcag
     4621 cgcagcttcc gctgggggat ttatgacaat ccctactgga cggtgaaccg aaatgaattc
     4681 aacgatgatg tcaatcgaat tatcagccag gcaaccgtaa cctgtgcacc aacttcctgg
     4741 ttgaatatca cttacaggtt gggtaacgat ttctatatcg accgccggaa aggaaccctg
     4801 gcgatcaatt cagccgaaca accgaccggg cagatcctgg aagatcatca taccaactgg
     4861 gacttcaatt ccgatctcat cttcaacttc aaaaaggata tcaaggatat ctcattgaat
     4921 ttcaatgtcg gacataacat cttccagcat tatcaccagc agatttatgt tcagggagat
     4981 aacctgacga tccccgatta ctataatatg gccaatgcca gttcggtgat tacgcgcgaa
     5041 actattacac agaaaaggac agccgcgttt tatggtgatt tcggatttgg atacaagagc
     5101 atgctgtacc ttaacctgac cggtagaaat gaatggtcga ccacattgcc ggaaaagaag
     5161 aattcattct tcttcccgtc ggccagcctt ggttttgtgt tcaccgaact gcctgcgctc
     5221 agggataaca aagtgttgtc gtttggtaaa ttgcgcacct cttatgccat tgttgccaac
     5281 gatgcaccgg aatacagcac agcaacttat tttatttcgt catactggat tgatggatgg
     5341 accaatggca tttcgttccc gtttgccggc cagaccgggt ttatgaacga tgacctgctg
     5401 ggaaatgaag acctgaaacc tgaaaggatg aaatcgtttg aagccggcac agagctccgt
     5461 ttcttcggca acaggctgaa tttcgacctg acctatttct ataataagaa tgaagacctg
     5521 atcattgtcg taccgctggc cggaagctca ggttatacgg cacaaacgat gaatgcagcc
     5581 accatggaaa acaagggcat tgaagcagtt cttggcggca gcccggtaaa aaacgacaag
     5641 ttggaatgga cgctgaatgt caactttaca aagattaaga atacggtgct ttctctggct
     5701 gatggcgtag aaagtgtcaa cctgggcggc tataccgggt cggatatccg tgctgttgca
     5761 ggaatgcctt atgctacgat ctatggttca caatgggtga aggatgataa cggaaatgtt
     5821 attattgatg accgtgaaac cattggcgga gaacccaacg accactacgg ctatcctttc
     5881 ctgagcgatg aggaagggcc gctgggatcg gcactgcccg actggacctg cggaatcagc
     5941 agcctcctga attacaaggg aatcatgttc tcattcctgt ttgatatccg gaccggggga
     6001 tacatgtgga acggcacacg cggagctctt tattcgttcg gtatgcatga agacaccgaa
     6061 gaccgcggca gtatgaccgt cttcgacggg attaaaggcc atccggtgac caatcccgat
     6121 ggaagtgtta cctatcagac gtccggtgta aacgatattg aggcgatcaa ggatgaaaac
     6181 tggtacaacg gcctgggtgg cggttttgcc ggtccgattg aaccgtttat cgaagagtcg
     6241 ggatgggtca ggctgcgtga agtttcactg gcttatacgt ttgataagat gctggaaggt
     6301 acattcatca agtcggccac actggtgctc acaggaaaaa acctgtggct cagcacgcct
     6361 tataccggga tcgacccgga aagcagcacg ttcggcgcca gcaatgccca gggcatcgat
     6421 tatttcaata cgcccaacac gagaagctat attgtatcgt taaaactgaa tttttaatct
     6481 aagaaaatct tgataccatg aaaaggatta gaaccaacat aataacggcg atcatacttt
     6541 cgctggctgt gttagtcacc tcatgcaaaa agtggatcga tccggaaatg aacgatgatc
     6601 ccaacaaacc gactgacgtt tcattgaacc tgctccttcc gtctatcgaa gccggaatcg
     6661 ggtatgcggt cggcggggac ctttcctatg ccacactgat gtggatgcag cagtattcgg
     6721 gcctgggcaa ccagcctatt gcattcgacc gttataatta tacgcagagt gatgtcgaca
     6781 atgtgtggaa atgggcgctg tatgcaggcg ttctgaaaga ccttcacacg atggccgata
     6841 aagccgacga ggagaactca ccttattacg gaggcattgc cagggtattg acggtttaca
     6901 tgctgggaac gactaccgat ctctggggcg atgtgcctta cagcgatgcc ttccagggaa
     6961 atgaaaacct taagccggtg tatgataccc agcaacaaat atatgatacg atggctgtta
     7021 tgatcaatga agccattgca gatctgactg ctgaaacgag caatttcgtt cccggcagcg
     7081 atgacctgat ttacggcggg gatctgagcc tgtggcttaa agcggcttat gccgtgaagg
     7141 cgcgttatgc tcttcatctt tcggaaaggg atgccaccgc ataccagaag gcgctagatg
     7201 ccctttccaa tgggataact tcaaatgaag aagatctcga atttcatttc gggactccgg
     7261 ccaatgagga aaatccgctc tatcagttcc tgatccagcg acccggtgat atcaccatgt
     7321 gcgccaattt tgtcaatatg ctgatccagg acgccgaccc gcgtctcgga cagttttgcg
     7381 atacgaccgg cggcaattac ggtgcgcatc ccggaaccgg cgacggcgat tgtccgccgt
     7441 caggatacta tacacagaat gattcgccgg taccctttgt ttcgtatgtg gaattgctgt
     7501 ttatcaaggc cgaaagcgaa tataaactgg gaattggcga cccgaaagtc acagttaaag
     7561 aagccgttgc acaatcgctg gcaaagttcg gtattgatga tcctgcatgg acagccgcgt
     7621 ttgctgcgaa aattgacgct ttgacaggag aggagcttta tgcggagatc atgcgccaga
     7681 aatacattgc cttattcaac caggtggaga cgtttaccga ttggcgccgt actggctatc
     7741 ctgaactgga accggcggct tatgcagcaa ccaatgatgg gcagctcccg agaaggtacc
     7801 cgtacccgac ttctgaaaga atttacaacg gcgataacat gcccgacggg cttaccctgt
     7861 cgaccagggt ctggtgggat ctctattaga ggtagtgaat ataaatactg ttagtcaccc
     7921 cgcttgaccg gatcggctcg ccgtaaacaa aaataacggc gtcgccatgt ccggcccggc
     7981 gggtttttaa taccttttcg ccggctttac ggatgaactc ttctggggtt tccggttgtt
     8041 ccatcagttc cggttgtatt ccataaagga gagataaccg gttcagcgtc gcaggcaggt
     8101 tgctgaaggc tataatgggc aggggtatgt cgagctggga gagataaaga gcaccgcctc
     8161 cgaattttga ccatatgatg atcagctttg catcgatgtc tctcacgata gattttacac
     8221 catgagccag ggcggcgctc cggtatttcg actcacgcaa ttgcccgggc acgtcacggg
     8281 caattttccg gcttctgatg tgttcgttcg tcaaacgggc gatccgggcc atcattctga
     8341 cagtttccac agggtatctg cctacggccg tttctcccga aagcataacc gcatccactc
     8401 cgtcgtaaat cgcgttggcg acgtcagaaa cctcggcgcg tgtcggaacg ggagattcga
     8461 tcattgattg cagcatctgg gttgcaacga tcacaggcac accctggtca tggcatgcac
     8521 tgatgatctt tttctggaga acggcgacct ctgccagctc catctccacg cccaggtcgc
     8581 cccgggcgat cataatggca tcactctccg ccacaatccc atcaaggtca tcgatagcct
     8641 gcggtttttc aatcttgctg atgatcggga tgaactgcac atcatcgggc acaccgctca
     8701 tgtcgtcggc atggtattcg aacgggctga aaggtctggc acccagggca gccagtatct
     8761 ctttcagatg aatgatgtct tttgcatgcc tgacaaaact caatgccagg taatcaactt
     8821 tcttcgcgac ggcaaattca acacaccggt catcccactc tgtcattgct gcgacggtca
     8881 ggtcggtttc gggaaggttg atcccttttt ttgaagatac cctgcctgcc tgcatgactt
     8941 cgcaaacgag ccggtcttca tgtttgccgg tacatatcag gaccacattc ccgtcgtcga
     9001 tcattatccg ctgaccggtc ctgacttcgc agacaatctc aggcgaagtc gtcgaaaaaa
     9061 ctacagtgcc gtcctctttg cgaaaagcag gtgtatcctg tgctgtgaat tccacttttt
     9121 cgcctttgat caattctatt ccgggatcgg ggacctggcc tatcctgatc ttcggcccgg
     9181 acagatcgcc aaggatggcc gccggcacac ccgacaaagc ggcggcctgg cggacattat
     9241 caatgatccg cgagaaagaa tctatactgc catgcgaaaa attcaccctg aacacacgca
     9301 caccttcccg gatcagttgc ccgaccaact caaccgacga actggaaggg cccagggtgg
     9361 caatgatctt tgtcagcagg attgcattat ccatcctcgg tatattaata ttaaaatttg
     9421 tggatatact ggagctgcac gctccgcata ggttctgctt tcagcggccg gagcgagtat
     9481 atattgttca gcatattgtt gaccagcagg ctgaattcgt tatggtcact caaggcataa
     9541 cccgccctga tatccaggat ccattgcccg ttattgtatt ggttgaaata atccatatac
     9601 cttatt
//
LOCUS       DAOPNH010000119         9601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7764_length_9601_cov_5.492353, whole genome shotgun sequence.
ACCESSION   DAOPNH010000119 DAOPNH010000000
VERSION     DAOPNH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7764_length_9601_cov_5.492353"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKZ74_07640"
     CDS             complement(<1..1319)
                     /locus_tag="PKZ74_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPM92670.1"
                     /translation="MNRFSRYTPIRIAFPFMAGVALFFLTGEIFSVFTLLTLACLFLA
                     GLCIHYLFFRANFRWRWTAGVTTACCLAAFGNIVTCLHDPQHVAGSNNFGPRPDTSIL
                     LLRAGHTPEKKTRSYALKVKVVAMLTDTTAWERSGLKMLVYFTDSSCADIKYGDVIIT
                     RAVVQPISPPGNPNVFDFKKHMYNQGYTHQIFLKPHQWGKLNTSRSNPVRMYAEMCRA
                     RFLDVLRKYRVEGEQFALVTALMIGERAYLEPETVREFSYAGAIHVLSVSGLHVGIMY
                     VLLDKLLFFLKRGKHGRKLQYILIIAGIWAYAVVTGLPSSVVRAALMFSIVAMANLFR
                     RDSDNFNSVATAAFLQLFINPFELTQIGFQLSYLAVLGIFAFYRPFNNMVVTGNKAVA
                     WIWSVIAVSLAAQLATGPLSVYYFNMFPVYFLITNLIVVPLASFITY"
     gene            complement(1365..2042)
                     /locus_tag="PKZ74_07645"
     CDS             complement(1365..2042)
                     /locus_tag="PKZ74_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92671.1"
                     /translation="MKTKYLKLFERYYNGELDEPEKEMFQSQLQNDHEMADAYREYLS
                     IYEALKDKETLEFRTKLNEIRDEFRHNRKKDRFMNDKTNWLLVAALFILIAGITVITS
                     FFVLTLKEQKKYVASVEAEFFKEYVALDAELMRFDQRNVDFKLLEPASPFSIDRNKPL
                     QLSWTANSDDPLIMELINWDGRIIYSSRGTVKSPYVIKGPLPAGIFVYRFRTSTQAYG
                     LGFLFLL"
     gene            complement(2039..2602)
                     /locus_tag="PKZ74_07650"
     CDS             complement(2039..2602)
                     /locus_tag="PKZ74_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92672.1"
                     /translation="MVHYTKYGIIEGIRRKDKDVLRYVYKQYFPMVRYFIVRNNGTEQ
                     DAEDIFQEAMIVVFERARKRNLALGCAFKTYLYSVVRHIWLQLADRNRLQYELSDMDE
                     FLLLEEKEIYDDFHYKKVIFQRKFLELTELCRKLLLMVVEGKPYEEIATILGYKGKQY
                     AVKRKYECMKSLINRVLNDPEYKNLTK"
     gene            complement(2750..3508)
                     /locus_tag="PKZ74_07655"
     CDS             complement(2750..3508)
                     /locus_tag="PKZ74_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPM92673.1"
                     /translation="MEKIVDNQYYDAAIEANMAVVLLKTRAFDLITSVPDSDELMEFI
                     RDAEHNPKVKALMLLNEPDCLGEKVYDEFIRSILKSDQNSENSDVPAFLQKNTRFRQI
                     INLNKLIRYLAGYQKLVVVGVSSTIVTPFMGVALVADLRLASPKGCFALAHTRYGLHP
                     SGAIPFFLTHYLGHSKALEVQLSERVCAEEAFRLGIVNQILPSEEFYSNCIRFVQPYL
                     KHCRSTLAMTKRLNNFKNRSLQEYLDHEASLMNL"
     gene            complement(3654..3730)
                     /locus_tag="PKZ74_07660"
     tRNA            complement(3654..3730)
                     /locus_tag="PKZ74_07660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3694..3696),aa:Glu,seq:ttc)
     gene            complement(3803..4483)
                     /locus_tag="PKZ74_07665"
     CDS             complement(3803..4483)
                     /locus_tag="PKZ74_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92674.1"
                     /translation="MSPLQNAGLYQIVNNKGKLKKSVYHNTLESLNLIKKTIGEMLED
                     YRREFAKDPEAIPFVVKDMGEFEIHLQFAGDVLIFNMHTNVFEFSRYHDVMSTSYIRE
                     DKARSYCGIINIYNFLADSFKYDRANDIGYLIGRIFINKDKSYFIEGKREVGLLYNNF
                     GQEKLDETAVRKVVESAVLYTINFDLLTPPFDSVKEISLSEMKSIAGSQTLKTGKRLG
                     FKFQADRD"
     gene            complement(4501..5268)
                     /locus_tag="PKZ74_07670"
     CDS             complement(4501..5268)
                     /locus_tag="PKZ74_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM92675.1"
                     /translation="MRKVVCFIILIVLLTDAVISRGQGQVTNLPKYDHADYHFGFTIG
                     MNQMLFDVKPSDDLNTRFYYESLSQTGELIADSATVLGVNGTPTFGFNIGIVGDKRLG
                     RYFNVRFVPALVFGERYLNYSVRAWANGEEKLVDIKKNIGSVFIDFPFTIKYKSKRHN
                     NYLAYLLAGGKYTLDIASNAKKKDSNGQVVVKLNKHDIYAIVGVGFDFYNPWFKMGVE
                     LKMSYGLLDVLKDDKTFFTQSIDKINSKVFELGVTFE"
     gene            complement(5380..6387)
                     /locus_tag="PKZ74_07675"
     CDS             complement(5380..6387)
                     /locus_tag="PKZ74_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPM92676.1"
                     /translation="MAVHDFHAIFSDFQKMKVIVIGDVMIDSYILGRVDRISPEAPVP
                     VVTVEKRYDRLGGAANVALNIKALGAEPLLYSVIGIDERGALFRKMIEDEGISVSGII
                     SCNTRITTSKSRILGNNFQMLRVDEEVTDDLTPENEERLLNTLSEAILKGNVNAIILQ
                     DYNKGVLTPKVIHETLSLAASRNVPVAVDPKKKNFLLYRNADLFKPNLKELRDGLGLE
                     MAAAEADQLKQASEWLHNRQGIKTVLVTLSERGVFISRKNGDEPFEQMIMPAYVRKIS
                     DVSGAGDTVISVAALALAAGLEPGLLAGLANLAGGLVCEEVGVHPVNRARLLSEARLL
                     L"
     gene            complement(6409..7611)
                     /locus_tag="PKZ74_07680"
     CDS             complement(6409..7611)
                     /locus_tag="PKZ74_07680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM92677.1"
                     /translation="MELVAQRILNLSESETLAMTRRSRELAAEGIDVINLSIGEPDFN
                     TPEPIKQAAIKAIHDNKTHYPPVPGYIELRKAICHKLKRDNNLDFTPEQIVVSTGAKH
                     SIANVIISLVNAGEEVVLPAPYWVSYKEMVKLARGVAVFVPATVETDFKITPEQLEAA
                     ITPKTKVVLYSSPCNPSGSVYSREELEALARVVARHPQVVIISDEIYELINFDCKHES
                     IAQFDFIRNQVVVVNGVSKGFAMTGWRLGYLAAPLWIAKACDKLQGQMTSATSSISQY
                     AAIEAMLTEPGKSPDIRDMVFTFRQRRNKMLDLLKTIPGLVTNVPKGAFYFFPDVTFY
                     FGKKYGERTISNACDLANYLLEVGHVALVPGDAFGNPDCIRISYATSMELLTEAVERM
                     KKALSELK"
     assembly_gap    7722..7821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7822..8409
                     /locus_tag="PKZ74_07685"
     CDS             <7822..8409
                     /locus_tag="PKZ74_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92678.1"
                     /translation="EPGTWNSETTDYYLCKYFLDMALTILSWILKFVLPSVVVFLAVR
                     MAVKAYMENEERKRMAKAGPDNSAIVLPIRLQAYERFILLLERMAPAQAINRALEPGM
                     TSFGLQLVLVRNIREEFEHNAAQQLYISQDSWVMVKSAKEAVIHLINSSASQTEASSP
                     SSELARIILERWSEMEHNPVQKAIDQLKSEVTGLL"
     gene            complement(8430..8936)
                     /locus_tag="PKZ74_07690"
     CDS             complement(8430..8936)
                     /locus_tag="PKZ74_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM92679.1"
                     /translation="MTVIEFNYKLLSMQSYLQYFAKKLTNNEEDANDLVQETNYKALL
                     FREKYVHHTNFKAWLFTIMKNVFINNYRRTKKARTFIDNTDNLHYIHQHIDVHPVTPD
                     SELREKEVRKAVSQLEDEQRIPFEMHTQGFKYKEIAEELDISIGTVKSRIFFGRKKLM
                     DKLEGYGN"
     gene            complement(9147..>9601)
                     /locus_tag="PKZ74_07695"
     CDS             complement(9147..>9601)
                     /locus_tag="PKZ74_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92680.1"
                     /translation="NPAHEHFITNLVKQKIYVAIDSIPVPAGAEVRKFLLFLPEWDLH
                     ELGLLVYDYILKSRGFKVIYLGSNVPEEDVYSVADYLKPDYFLTAFANAIEPEKLESY
                     INRVAGRFPGSKIFITGFQAQAMPPAFPHNVVRVNSAQQFSSEILPGL"
BASE COUNT         2595 a         2214 c         2109 g         2583 t
ORIGIN      
        1 aaataggtaa taaaagaggc caggggaaca acaatcaggt tagtgatcag gaaataaacg
       61 gggaacatat taaagtaata tacggatagc ggacccgtag ccagctgggc agccaacgaa
      121 acggcaatga ccgaccatat ccaggcaacg gctttattgc cggtaaccac catgttattg
      181 aaaggccggt aaaatgcaaa aatgccgagc acagccaggt aagataactg gaatccaatc
      241 tgggttagtt caaagggatt gatgaagagc tgaaggaaag cggcagtagc caccgaattg
      301 aaattatccg aatccctgcg gaataaattc gccatagcta caatactgaa catcagtgca
      361 gccctgacaa cggaggaggg aagtccggtc actactgcat aggcccatat cccggcaata
      421 attaaaatat actgcagttt gcggccatgt tttccacgct tcaggaaaaa gagcaatttg
      481 tcgagcaaga catacataat cccgacatga agccctgaaa cacttaacac atgaatagcg
      541 ccggcataac tgaattccct gaccgtttca ggttctagat aggccctttc cccaatcatc
      601 aaagcggtaa caagcgcaaa ttgctcaccc tccacccggt attttctcag aacatccaga
      661 aatctggccc tgcacatctc cgcatacatt ctaacaggat tagagcgact ggtattcagc
      721 ttgccccatt gatgcggctt gagaaagatc tgatgggtat atccctggtt atacatatgt
      781 tttttaaaat caaaaacgtt tggattgccc ggtggagaaa tcggctgaac aactgctctg
      841 gtgattatta cgtcgccgta cttaatgtcg gcacagctgc tgtcggtgaa atagaccagc
      901 attttcaaac ctgatctttc ccaggccgtt gtatcggtca acattgccac caccttaact
      961 ttaagggcat acgaccgggt ctttttttca ggtgtatgcc cggctctcag caataaaata
     1021 ctggtatccg gcctgggtcc aaagttgttg ctgcctgcaa catgctgtgg atcatgcagg
     1081 catgtgacaa tatttccgaa ggcagccaag caacaggccg tcgtaacacc tgctgtccat
     1141 cgccagcgaa aattcgccct gaaaaaaaga taatgaatac acaagccagc cagaaagagg
     1201 cacgcgaggg ttaataaagt aaaaacagaa aagatttcac cggtcaggaa gaacagggca
     1261 acgcctgcca tgaacgggaa ggcaatcctg atcggagtgt accggctgaa cctgttcaat
     1321 ggcaatgatt ccgttatttg acacagcgcc cctcatcatc ctgctcacag caaaaacagg
     1381 aatcccaaac catatgcctg tgtggaggtc ctgaatcggt aaacaaaaat acctgccggc
     1441 aatggccctt tgatcacata cgggcttttc acagtccccc tggaagaata tatgatcctt
     1501 ccgtcccagt tgatcagctc catgatgagc ggatcatcag aattagcagt ccagctcaat
     1561 tgcaggggtt tattccggtc gatggagaac gggctcgccg gctccagcag tttaaaatcg
     1621 acattgcgct gatcaaatct catcagctct gcatcgaggg ctacatattc cttaaaaaat
     1681 tccgcttcga cagaggcgac atatttcttt tgttctttca gagtgaggac gaaaaatgag
     1741 gtaatgacgg ttattccggc aatcaggata aaaagtgccg ccaccaaaag ccagttggtt
     1801 ttatcgttca taaaccggtc cttcttcctg ttatgcctga attcatcccg tatttcattc
     1861 agtttggtcc ggaattccag tgtttccttg tccttcaatg cttcataaat actcaggtat
     1921 tccctgtaag catccgccat ttcatggtcg ttctgcaatt ggctttgaaa catttccttt
     1981 tcaggctcat ccaattcgcc attgtagtat ctttcgaaca attttaaata ttttgttttc
     2041 attttgtcag atttttatat tcaggatcgt tcaataccct gttaattaat gatttcatgc
     2101 attcatattt gcgtttcaca gcatattgtt tgcctttata ccccagaata gtggcaattt
     2161 cttcataggg tttgccttcg acaaccatca gcagcagctt cctgcataat tcggtcaatt
     2221 caaggaattt tctctgaaaa atgactttct tatagtggaa atcgtcatag atctcttttt
     2281 cttccagcaa aagaaactca tccatatcgc tcagttcata ctgcaaccgg ttccggtctg
     2341 ccagttgtag ccagatatgc ctgaccacag aataaagata ggtcttaaaa gcgcaaccca
     2401 gggccaggtt tctctttctg gcccgctcaa agacgacgat cattgcctcc tggaatatgt
     2461 cttccgcatc ctgttccgtt ccgttattcc tgacaataaa gtacctcacc atcggaaagt
     2521 attgcttgta gacatatcgc aggacatctt tatccttacg ccggattcct tctataatcc
     2581 catatttggt atagtgtacc attctatact gtttatccgg tttttcacaa aaaaataccc
     2641 ggtcgtttta aaatattttt aaattatttt ttaatatatt gctttacaat acattacaaa
     2701 ggcggcataa aggtagtttt tgtccttttt tcaggaaaaa acttataatt cagaggttca
     2761 tcaggctggc ttcatggtcg aggtattcct gcagcgaacg gtttttgaaa ttattaagcc
     2821 tttttgtcat cgccagcgtt gatcggcaat gtttcagata cggctggaca aaacggatgc
     2881 aattggaata aaattcttcg gaaggaagaa tctgattaac gatacccagg cgaaaagcct
     2941 cttcagcgca aaccctttcg gaaagctgaa cttcaagtgc ttttgaatgg ccgagataat
     3001 gtgtcaggaa aaaggggatg gcgccgctgg gatgcaatcc ataccgggta tgggccaggg
     3061 caaaacatcc ttttggactg gccaggcgca aatcggccac cagggctact cccatgaatg
     3121 gagtcacaat cgtgctgctc acgccgacta cgaccagttt ctggtaaccg gccaggtacc
     3181 ggatcagttt attcagattg ataatctgcc ggaaccgggt atttttctgg aggaaggccg
     3241 gtacgtcgct attttcggaa ttttggtcac tcttcaaaat gctccggata aattcatcat
     3301 acactttctc tccgaggcag tcgggctcat tgagtagcat caacgctttt acttttggat
     3361 tatgctcggc atcccggata aattccatca gttcatcgct gtcgggcacg ctggtgatca
     3421 ggtcaaaggc gcgggttttt agcagaacga cagccatatt agcctcaatg gctgcgtcat
     3481 aatattgatt atcaacaatc ttttccatac aatccggcat ttttggtcgg ggttaaattc
     3541 aacgacaatt tacaaaattc ggataaataa tcatcaacaa aaaaagggga aaccggtaaa
     3601 attttatgga aaggataagg tcgaaataaa aaaagccggc atgaaaccgg ccctggtagc
     3661 ccgtagggga ttcgaacccc tgatttccag gatgaaaacc tggcgtccta accacctaga
     3721 cgaacgggcc aaaatttgag ggtgcaaaaa tagcaagatt tttttatcct gcaaaacact
     3781 ttgaaaaaaa tctttacttt tcttaatcgc ggtcggcctg aaacttgaaa cccaatcgtt
     3841 taccggtttt aagcgtctgg ctgccggcga tcgatttcat ctccgaaagg gaaatttctt
     3901 tcacggaatc gaacggaggc gtcagcaaat cgaaattaat ggtgtataaa acggccgatt
     3961 cgacgacctt ccggacagcg gtctcatcca gcttctcctg gccgaaatta ttgtacagaa
     4021 gcccgacttc ccgcttgccc tcaatgaaat agctcttatc cttattgata aaaatcctcc
     4081 cgataagata gccgatatca ttggcccggt cgtatttaaa tgaatcggcc aggaaattgt
     4141 aaatattgat aatgccacag tacgaccttg ctttgtcttc ccggatgtaa gaggtcgaca
     4201 tcacatcatg atacctcgaa aattcgaaaa cattggtatg catgttgaag atcaggacat
     4261 caccggcaaa ttgcaggtgg atctcgaatt cccccatatc tttgacaacg aacgggatag
     4321 cctcaggatc ttttgcaaat tcccgccggt aatcctccag catctccccg atagtcttct
     4381 tgatcaggtt aagcgattcg agcgtattat ggtaaacact ctttttgagc ttgcctttgt
     4441 tgttgacaat ctggtaaagt ccggcattct gtaatggcga catggcacat aagattttga
     4501 ttattcaaag gtaactccca gttcgaatac tttggaattt attttatcga tgctctgtgt
     4561 gaaaaaggtt ttatcatcct ttaaaacatc cagtaacccg tacgacatct ttaactccac
     4621 tcccatcttg aaccagggat tgtaaaaatc aaaacctaca ccgacgatag catagatatc
     4681 atgcttgttc agcttgacca ccacctgtcc gttggaatcc tttttcttgg cattggaagc
     4741 aatgtcgagc gtgtatttcc ccccggccag gaggtaggcg agatagttat tatgcctttt
     4801 ggatttgtat ttgatggtaa aaggaaaatc gatgaaaacc gatccgatat tcttctttat
     4861 atccaccagc ttttcctcgc cgttcgccca ggctctcaca ctgtaattaa ggtaacgttc
     4921 gccgaaaaca agtgcaggga caaaacggac attaaaatag cgtccgagtc ttttatcccc
     4981 gacgatgccg atgttgaatc caaaagtggg tgtaccgttc actccaagga cggtagccga
     5041 atcggctatc aactctcccg tttgactgag cgattcataa tagaaccggg tattcaggtc
     5101 atcggaaggc ttaacatcga acaacatctg gttcataccg atagtaaaac caaaatggta
     5161 atcggcatgg tcgtatttag gcagattggt cacctggccc tgaccccggg aaataacagc
     5221 atctgtgaga agaacgatca gtattatgaa gcaaacaacc tttctcaact ctatccggat
     5281 taaatttaaa aagtaacgac tggaaacctg ctttattttg agggtgcaaa cttaatgaaa
     5341 actttgaaga cggaaagcat accgttcaac ggaaaaggtt cagaggagaa gccgggcctc
     5401 ggaaaggagg cgtgcacggt taaccgggtg aacgccaacc tcttcacaga ccagccctcc
     5461 tgccaggttc gccagtccgg ccagcagccc gggttccagg cctgctgcca gcgccagggc
     5521 cgccacgctg attaccgtat cgccggcgcc ggagacatcg gagatcttcc tgacataggc
     5581 cggcatgatc atttgttcaa agggctcgtc cccgtttttc cggctgataa agactcccct
     5641 ctccgacagg gtgaccagga cggtttttat tccctgcctg ttatgcagcc attccgatgc
     5701 ctgtttgagt tgatctgcct ctgctgcagc catttccagg cccagaccgt ctctgagctc
     5761 tttcaggtta ggcttgaaca ggtccgcatt cctgtaaagc aaaaaattct tcttcttggg
     5821 atccacagcc accggaacat tccggctcgc ggccagcgac agggtctcat gaattacttt
     5881 tggcgttaat acccctttgt tgtaatcctg aaggataatg gcattaacat tgccttttaa
     5941 gattgcttct gaaagggtat ttagcagtcg ttcctcattc tccggggtca ggtcgtccgt
     6001 cacctcttca tccaccctga gcatctgaaa attatttccg aggatgcgtg acttggaggt
     6061 ggtgatcctg gtgttgcagg atataattcc ggaaaccgaa ataccctcat cctcaatcat
     6121 tttccggaaa agcgctcccc tttcatcgat cccaatgacc gaatataata gcggttccgc
     6181 tcccagggct tttatattca atgcaacatt ggcggcgcct ccaagccggt cgtagcgctt
     6241 ttctactgta acgaccggca caggcgcttc gggagagatc cggtcaacac ggccgagaat
     6301 gtaagagtcg atcatgacat caccgatgac gatcactttc attttctgaa agtcgctgaa
     6361 tatcgcatgg aaatcatgaa ctgccataat accttttacg gctgaaccct atttaagttc
     6421 cgataaggct ttcttcattc tttcaactgc ttcggtaagc agttccatcg aagtggcata
     6481 ggaaatcctg atgcagtccg gattaccgaa tgcgtcaccg ggcaccaggg ctacatggcc
     6541 cacttcaagg aggtaattgg ccaggtcaca ggcattgctg atggttcgct cgccatattt
     6601 ctttccaaaa tagaaggtaa catcggggaa aaaatagaag gcgcctttcg gtacattcgt
     6661 caccaggccc ggaatcgttt taagcaagtc cagcatttta ttcctccgct gccggaaagt
     6721 aaagaccata tcgcggatat cgggcgattt gccgggttcg gtcagcatcg cttcgatagc
     6781 ggcatattgt gaaatggaac tggtggccga ggtcatctgg ccctggagct tgtcacaggc
     6841 tttcgcaatc cacaaaggag cggccaggta tcccaggcgc catccggtca tcgcaaaccc
     6901 tttggaaacc ccattcacca ccacgacctg gttgcggata aaatcaaact gggctatgct
     6961 ctcgtgcttg cagtcgaaat tgataagctc gtaaatctca tcggagatga tcactacctg
     7021 cggatggcgt gcgacaaccc ttgccagcgc ttccagctcc tccctggaat atacgcttcc
     7081 ggaaggatta caggggctgc tgtacaggac caccttggtt ttaggtgtaa tggcggcttc
     7141 cagctgttca ggcgtgatct taaaatcggt ctctaccgta gcggggacaa aaacggccac
     7201 tccccgtgcc agtttcacca tttctttgta agagacccag taaggggcag gaaggactac
     7261 ctcttccccg gcattgacca gactgatgat gacattggca atggaatgct ttgcaccggt
     7321 cgatacaacg atctgttcgg gggtaaagtc aaggttatta tctcttttca gtttatggca
     7381 gatggctttt ctcaattcga tatagccggg taccggcgga taatgtgttt tattatcgtg
     7441 aattgcttta atggccgcct gtttgatagg ctccggggta ttgaaatcag gttcgccgat
     7501 actcaggttg atcacatcaa tgccctcggc tgccagttcc cggcttcggc gcgtcatggc
     7561 cagggtttcc gactcagata aattcagtat acgttgtgca acaagttcca tagatcattt
     7621 cattgttaaa agattcacaa aaatagaaaa ttatccttaa caacgtatta tcgacggaaa
     7681 gggaaaaggg aatagtaatt tggaaatttg gaaatttgga annnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn ngaacctgga acctggaact cagaaacaac agattactat
     7861 ctttgcaaat attttttaga tatggcgctg acaattttat catggatcct gaagttcgtt
     7921 ttgccatcgg tggttgtctt cctggcggtt agaatggcgg taaaagctta catggaaaat
     7981 gaggaaagga agaggatggc taaggccggt cctgacaatt cggctattgt tttacccatt
     8041 cggcttcagg cttatgaaag atttattctt cttctggagc ggatggctcc ggcgcaggcg
     8101 atcaacaggg cattggaacc aggtatgaca agcttcgggc ttcagctggt tttggtgaga
     8161 aacatccggg aggagtttga gcataatgca gcccagcagc tttatatttc gcaggatagc
     8221 tgggttatgg taaagtcggc caaagaagcg gtcattcatc tgatcaattc ttccgcctca
     8281 caaacagaag ccagctcacc cagtagtgaa ctggcccgaa tcattttgga aaggtggtct
     8341 gaaatggaac acaacccggt tcagaaagca atagaccaac ttaaatcaga agtaaccggg
     8401 ctactctagc caaaaacgtt cccttttccc taattcccgt acccctcgag cttgtccatc
     8461 agcttctttc tgccaaagaa tatcctgctt ttcactgtgc cgatcgaaat atcgagttct
     8521 tcggcaatct ccttgtattt aaaaccctgc gtgtgcattt cgaacggaat cctttgctcg
     8581 tcttcgagtt ggctgacggc ttttcttacc tctttctcgc gaagttcgga atcgggtgtt
     8641 acaggatgca catcaatgtg ctgatgtata tagtgcaggt tatccgtgtt atcgataaaa
     8701 gtccgcgctt ttttcgtccg cctgtagttg ttgatgaaca catttttcat aatagtgaac
     8761 aaccaggctt taaaattggt atgatgtacg tatttttcac gaaacaataa ggctttgtaa
     8821 ttcgtttcct gaaccaggtc gttagcatct tcctcattgt tggtcagctt tttagcaaag
     8881 tactgaagat aactctgcat gctgagaagc ttataattga attctattac tgtcatggct
     8941 cgtatatttg tttaattatt atgcgacaaa gttaaacata aatacgatca cttgtcaagt
     9001 tttttaacat ttttttaaga attattctaa ataagaatat tgttagcatt gttattcaat
     9061 gctttaaagt atttaaagta atcagaattt aaatagaatt gttaaacgat gttaaaatat
     9121 cagtcgataa ggtttaacag aaacggttat aaaccgggga ggatttcgga agaaaactgc
     9181 tgagccgaat ttacccggac tacattgtga gggaatgccg gcggcatagc ctgagcctga
     9241 aaaccggtaa taaagatttt tgaaccggga aagcggccgg caaccctgtt gatatatgat
     9301 tccagttttt caggttcaat tgcattggca aatgcagtca gaaaataatc gggcttcaga
     9361 taatcagcca ctgaataaac atcctcctcc ggcacattcg agcccaggta gattacttta
     9421 aaaccccggc ttttaaggat atagtcgtaa accagaagac ctaactcatg cagatcccat
     9481 tcgggaagga acagtaaaaa tttcctgact tcggctccgg caggcacggg aatactgtcg
     9541 atggcgacat aaattttctg cttaacgagg ttagtaatga agtgctcgtg cgctggattg
     9601 a
//
LOCUS       DAOPNH010000120         9588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7775_length_9588_cov_4.899402, whole genome shotgun sequence.
ACCESSION   DAOPNH010000120 DAOPNH010000000
VERSION     DAOPNH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7775_length_9588_cov_4.899402"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..837
                     /locus_tag="PKZ74_07700"
     CDS             238..837
                     /locus_tag="PKZ74_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPM92681.1"
                     /translation="MTSKEDKNKKTETTEDQSVKTGSDSHSGPASEESPAKKDKKKKH
                     DDSEELNRQWQAKYDEVNDKYLRMYSEFDNYRKRTLKEKVEFSKTASEEIIVALLPVI
                     DDLERALATGFKTEDGKEVVMMEGLTLIYQKFKGLLAQRGLEPVPSVGEAFDVDFHDA
                     LTNIPAPSDELKGKVIDEIEKGYKLNGKVIRYSKVVVGN"
     gene            839..1993
                     /gene="dnaJ"
                     /locus_tag="PKZ74_07705"
     CDS             839..1993
                     /gene="dnaJ"
                     /locus_tag="PKZ74_07705"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPM92682.1"
                     /translation="MAKRDYYEVLDIPRSASADEIKKAYRKMALKYHPDKNPDDPSAE
                     AKFKEAAEAYEVLSDSDKKQRYDRYGHDGVKGMNGGGFNMSMDDIFSHFGDIFGGGFG
                     SAFGEQFGFGTRGRTTRRVNRGTNLRVKVKLTLEEIASGVEKKIKVSKYIPCDHCNGT
                     GAEKGASMNTCPTCHGQGRVTRVTSTFLGQMQTSSTCPTCDGEGTIIGTKCNKCFGNG
                     IVKGEEVIAVRIPAGVADGMQLSMSGKGNAAARGGIPGDLLILIEEHEHEHFTRDNNN
                     LVYEHFISLPDAALGGSVEIPTLDGKARLKIDPGTQSGKILRLKNKGLPDINGYGRGD
                     LLVNLTVWTPRELTREEHQIMEKLRESENFKPHPSKKDRGFFDRMKDLFQ"
     gene            1990..2907
                     /locus_tag="PKZ74_07710"
     CDS             1990..2907
                     /locus_tag="PKZ74_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92683.1"
                     /translation="MNIIQVEEVTKRFSGHTALEKVTLDVPAGSIYGLLGPNGAGKTT
                     LIRIINMITAPDEGQILFDGKKIHPDDITRIGYLPEERGLYKKMKVGEQALYLSQLKG
                     LSHTDAQKQLKEWFEKFEISAWWNKKVEELSKGMQQKVQFIITVLHKPKLLIFDEPFS
                     GFDPINANLLKEEILKLRSDGSTIIFSTHNMASVEELCDNIALINKSRKILEGSMMEI
                     RQQHRTEIYEVEVSGLVNGSLELPAQCEIIKKEEKEGRQTLQIRGILDHKPNELLSGL
                     ISQASVHAFREILPTMNDIFIATVNAAEK"
     gene            2904..4262
                     /locus_tag="PKZ74_07715"
     CDS             2904..4262
                     /locus_tag="PKZ74_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92684.1"
                     /translation="MKKIWLIIQREYLTRVKKKSFIVMTFLGPILMASVWIVPLLLAS
                     RSDGIKTIEVLDETGIFFEEFKETSDTKFEYITDDLQTAKEKLATSENFAILYIPKSS
                     YSTPLAFYLYSYKNPGLSLKLYLQESANKILENKKLQDVYGIDEETLASVKSNINLVV
                     EDIETNKKSFTEISWALGFIGGMLIYFFIFMYGAQVMRGVIEEKTNRIIEVIVSSVKP
                     IQLMLGKIVGIAMVGLTQFLLWILLTLGIVTAFTAAYSDQIADFKMGEQPVVTDPGQL
                     FQSDAMGMPDTQQGLEALGSDENINQILEVFFSINYGIIIFTFLFYFVGGYLLYAALF
                     AAIGSAVDNEADTQQFMMPVTIPLIFSIIIGLSFVLNNPDGSVAYWLSLIPFTSPIIM
                     MIRVPFGINIYTDLIPSMILLIVGFLVTTWIAAKIYRTGILMYGKKVSYRELWKWLTY
                     KP"
     gene            complement(4263..5282)
                     /locus_tag="PKZ74_07720"
     CDS             complement(4263..5282)
                     /locus_tag="PKZ74_07720"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPM92685.1"
                     /translation="MEKKDLLKQAIEHVDIKSFDSAPVIDSMRKMSFTSRETANAADI
                     LQLMINDKSCSIWLTLAGSTSAGGCMQVYVDMVKNNMIDAIVATGASIVDMDFFEALG
                     FRHYRGTPSIDDRMLRNNYIDRIYDTYIDEEELQHCDMTVKLIADKLEPRPFSSREFI
                     REMGRYLSENSVKKDSLIQACFEHDVPIFCPAFSDSSAGFGLVKHQWENPGKHVSIDS
                     VKDFLELTLVKMAAESTGLFMIGGGVPKNFAQDTVICAEILGKDVPMHKYAVQITVAD
                     VRDGACSSSTLKEASSWGKVDTVYEQMVYAEATSVLPLIVSYAYHRGDWKSRTKYRWS
                     KLFDK"
     gene            complement(5376..6224)
                     /gene="speB"
                     /locus_tag="PKZ74_07725"
     CDS             complement(5376..6224)
                     /gene="speB"
                     /locus_tag="PKZ74_07725"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPM92686.1"
                     /translation="MNFGGIPAELSGYEVSPIVILPVPFDATSTWIKGADRGPDAILE
                     ASANMELYDIETDSEVYLKGIFTAEPVIEENSPENLAVKVKKSISRFLDDGKFVVTLG
                     GEHSVTIGAVQAFAERFPGLHVLQIDAHTDLRPEYEGSRFNHACVMSRVRELCPIVQV
                     GIRSMDVVEKPFVDKGRIFYAKDIAGSGNNWIDRVIELLGDQVYITIDLDGFDPSVLP
                     ATGTPEPGGLNYYEVLHLVRKLINTRKVVGFDVVELCPIVDNKASDFLASKLIYQMLS
                     YQFVNQ"
     gene            complement(6274..7665)
                     /locus_tag="PKZ74_07730"
     CDS             complement(6274..7665)
                     /locus_tag="PKZ74_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPM92687.1"
                     /translation="MKVKYLDLIEQTFDFPTNEFEVIEYELNFHEIPLMDIIKQYGTP
                     LKISYLPKISEQIQKAKRWFNVAMAKADYNGNYIYCYCTKSSHFSFILQEAMKNDIHI
                     ETSSAFDIHIVEQLFEEGRINKDTHIICNGFKRPQYIENIINLINRGFENTIPVLDNI
                     FELDKFSKGIRKKVKLGIRIASEEEPKFEFYTSRLGIRYNDIVSYYQEKIQNNNKFAL
                     KMLHFFINTGIRDTAYYWNELSKCVNVYCELKKICPELDSLNIGGGFPVKNSLTFDYD
                     YEYMAEEIISQIKIICNQNNVPEPDIFTEFGSFTVGESGAVLYSIIDQKRQNDREIWN
                     MIDSSFMTTLPDTWALNQRFILLAVNNWDAEYERVFLGGLTCDSMDYYNSEAHSNAIF
                     LPKMRPGIPQYIGLFHTGAYQESIGGYGGIQHCLIPAPKHIIIDRDSDGEYNTKLFAK
                     EQSYKAMMKVLGY"
     gene            7984..>9588
                     /locus_tag="PKZ74_07735"
     CDS             7984..>9588
                     /locus_tag="PKZ74_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPM92688.1"
                     /translation="MQSKGAIKVFAIAFALVCLFQLSFTFFSGITERKARKYATNAEA
                     MRLADELAGGSQRSKTFVMDSLAKARESYYLDSMANQPIYNILVKEYTYKEVKEREIN
                     LGLDLRGGMNVVLEVSIGEIVKALAGPNAVNPIFQQAMERTYEKQKSSDRDFVTLFGE
                     AFREVDPNANMASIFSTVEFKDKITYNSTNDEVLEVLRDETEGAFDMTYNIIRTRIDR
                     FGVTQPNVQKLTGTGRILVELPGIKEPARVRKLLQGTAKLEFWETYNYSEVYGYFADA
                     NSRLVTILGSQNLEDNGTAGKTREGETSDTGENGSSATGDTTKSLLDKIESDTSKAAQ
                     DKSFEEYAKDNPLNAYLMPAFFQGEDGQYYPGQSATVGFAAIKDTAKVNDMLRKVNSV
                     FPRNLKLVWTVKPRDANSDVLELVALKVTSRDGTPALGGEVIVDARQDYDQLGNVEVS
                     LNMNAEGARTWKRLTGENIDKQIAIVLDNHVYSFPTVRGEIPNGMSSISGGGMTLEEA
                     QDLSNILKAGKLPASARIVEEAVVGPS"
BASE COUNT         2766 a         2243 c         2162 g         2417 t
ORIGIN      
        1 ccagaacatc gattcacgac tggccgccct gggtgccgat atcaggcgca tctgaaatct
       61 gcatgccgtc ttttcctgtc ggggacttag ccctgccaaa ttgtcctttt atcgcggttg
      121 gcaaacaatt tgatcgcaaa gcgcgaagtt tcactatttt tgcacccttg taaagaagga
      181 atttattgaa tatcaatctt taaagggtgt atttattatt atagccagat aaataccatg
      241 acgagcaaag aagataaaaa taaaaaaacg gaaaccaccg aagatcaatc ggtaaaaacc
      301 ggcagtgatt cccattcagg accggcaagc gaagaaagcc ctgccaaaaa agataagaaa
      361 aagaaacatg atgattccga agaactgaac cggcagtggc aggctaaata cgacgaggtt
      421 aatgacaaat atctccggat gtactcagaa tttgataatt accgtaaacg gacactgaaa
      481 gaaaaggtag aattcagcaa aaccgcatcc gaagagataa ttgttgccct cttacctgtt
      541 atcgatgatc tggaaagggc cctggccacc ggctttaaaa ccgaagatgg caaagaggtg
      601 gtgatgatgg aagggcttac gcttatctat caaaaattta aaggattact tgctcagcgc
      661 gggcttgaac ccgtcccttc cgtcggggaa gctttcgatg ttgattttca tgatgcgctg
      721 accaatatcc ctgccccttc cgatgagctg aaaggtaaag tgatcgacga aatagagaaa
      781 gggtataaac tgaatggtaa agtgatccgg tattcaaaag ttgtagtggg gaattaagat
      841 ggcaaaacgc gattattacg aagttcttga tattccccgt tcggcttctg ccgacgagat
      901 caagaaagcc taccggaaaa tggccctcaa ataccatccg gataaaaatc cggatgaccc
      961 atcggccgag gctaaattca aggaagcggc agaagcatat gaggtgctta gtgattctga
     1021 taaaaaacaa cgttacgacc gttacgggca tgacggggtg aaggggatga acgggggcgg
     1081 cttcaatatg agcatggacg atatattctc ccatttcggg gatattttcg ggggaggttt
     1141 cggaagcgcc tttggggaac agtttggttt cggaacgcgg ggcaggacca cccgaagggt
     1201 gaacagggga accaacctga gggtgaaggt caaactgacc ctggaagaga ttgcgtctgg
     1261 agtagagaaa aagataaaag tcagcaaata catcccctgt gaccattgta acggaacggg
     1321 cgccgaaaaa ggagcctcga tgaatacgtg tccgacgtgc catggccagg gaagggtgac
     1381 ccgggtaacc tcaaccttcc tgggacagat gcaaacatca tccacctgcc cgacctgcga
     1441 cggcgaaggg acgattatcg gcaccaagtg caacaaatgc tttggcaacg gaattgtcaa
     1501 gggagaagag gtgatcgctg ttaggattcc ggcaggcgta gccgacggaa tgcaactctc
     1561 tatgagcggc aaaggcaatg cggcagcccg tggcggtatc ccaggggatc tgctcatcct
     1621 tatagaagaa catgaacatg agcactttac acgcgacaat aacaacctgg tctatgaaca
     1681 ttttatcagc cttcccgatg cagccctcgg cggttcagtt gaaatcccga ctctcgacgg
     1741 aaaagcaagg ctgaaaatag atccgggaac gcaaagcgga aaaatactgc gtttgaaaaa
     1801 caaagggctg cccgatatta acgggtacgg aagaggcgac ctgctggtca acctgaccgt
     1861 ctggactccc agggagctga cccgcgaaga acaccaaata atggaaaagc tcagggaatc
     1921 ggaaaacttt aagccgcacc cttcgaaaaa agacagagga ttttttgaca gaatgaaaga
     1981 tttattccag tgaacataat acaggtagaa gaagtcacca agcgtttttc cggacacacc
     2041 gcgctggaaa aagtcaccct cgatgtcccc gcaggaagca tctacggcct tctcgggcca
     2101 aacggagcag gtaaaacaac ccttatccga attattaata tgatcaccgc gccggatgaa
     2161 gggcaaatcc ttttcgacgg aaaaaagatc catccggatg atatcacccg cattggatac
     2221 ctgccggaag agaggggact ctataaaaag atgaaggtcg gcgagcaggc gctctacctc
     2281 tcgcaactga aaggattgag ccataccgac gcccagaagc agctcaaaga atggtttgag
     2341 aaatttgaga tcagcgcctg gtggaataaa aaagtggaag aactttcgaa aggaatgcag
     2401 cagaaagtac agttcattat cacagtcctt cataaaccca aactgctcat cttcgatgaa
     2461 cccttcagcg ggttcgaccc gatcaatgcc aacctgctga aagaagagat cctaaaactg
     2521 cgttccgatg gttctactat aatcttctct acccacaaca tggcttccgt tgaggagctc
     2581 tgcgacaata tcgccctgat caacaaatcc agaaaaatac tggaaggcag catgatggag
     2641 atccgccagc agcaccgcac cgaaatttac gaggtggaag tctccgggct ggtaaacgga
     2701 agcctggaac tgcctgccca atgtgaaatc attaagaaag aggaaaaaga gggaagacaa
     2761 actcttcaga tacggggaat tctggatcat aagccgaatg aactgctatc gggtctcatt
     2821 tcgcaggcca gtgtgcatgc attccgcgaa attcttccga ccatgaacga catctttatc
     2881 gccaccgtta acgctgctga aaaatgaaaa aaatctggct catcatacaa cgcgaatacc
     2941 tgacccgggt aaagaaaaag tcgttcatcg tcatgacttt cctcgggccc atcctgatgg
     3001 catcggtctg gattgtgcca cttttactgg cttcccgctc cgacggcata aaaaccattg
     3061 aggtacttga tgaaaccggt atttttttcg aagaatttaa ggaaacttcc gataccaaat
     3121 ttgaatatat aaccgacgat cttcagacag caaaagaaaa actggctacc agcgaaaact
     3181 ttgcaatcct ctatatcccc aaatcttctt actcgacacc attggccttt tatctttatt
     3241 cctataagaa tcccgggctc agcctgaagc tctacttgca ggaatcggca aataaaatcc
     3301 tggagaacaa gaaactacaa gacgtttacg ggattgatga agaaaccctg gcctcggtca
     3361 aatcaaatat caatctcgtt gttgaagata ttgagaccaa caaaaagtcg ttcaccgaga
     3421 tcagctgggc actgggattc attggcggta tgctgatcta tttcttcatt tttatgtatg
     3481 gtgcgcaggt tatgaggggc gttatcgaag agaaaaccaa ccggatcatc gaggttatcg
     3541 tatcctctgt taaaccgatc cagctgatgc tcggtaagat cgtcggcata gcgatggtcg
     3601 ggctgaccca gttcctcctc tggatcctgc tgacccttgg cattgtaacg gcattcacag
     3661 ccgcgtactc cgaccagata gccgatttta agatgggcga gcagcctgtg gtcaccgatc
     3721 ccgggcaact tttccagtcc gacgccatgg gtatgcccga cacgcagcaa ggtttggagg
     3781 ctctgggatc cgacgaaaac atcaaccaga tcctggaggt tttcttctcc atcaattacg
     3841 gaatcatcat ttttaccttc ctgttttatt ttgtcggagg atacctgctg tatgccgcgc
     3901 tgtttgcggc gatcgggtcg gctgtcgaca atgaggccga tacacagcaa ttcatgatgc
     3961 cggtaacgat acctctcatc ttttccatta ttatcgggct gtcgtttgta ttgaataacc
     4021 ctgacggaag tgtcgcctac tggctttccc ttatcccttt cacctcacct atcatcatga
     4081 tgatacgggt tcccttcggg attaacatat acaccgatct gatcccttcg atgatcctgc
     4141 ttatcgttgg attcctggta acgacctgga tagccgctaa aatataccgt accggtatcc
     4201 ttatgtatgg aaaaaaggtc agctacaggg aactgtggaa atggcttact tacaagccgt
     4261 gattatttat cgaaaagctt gctccagcgg tattttgtcc ggctcttcca gtctccgcga
     4321 tgataggcat agctcacaat aagcggcagt acagaagtag cttcagcata aaccatctgc
     4381 tcgtaaaccg tatccacctt tccccaggag gaagcttcct taagggtaga actcgaacag
     4441 gccccatccc tgacatccgc taccgtgatc tggacagcgt atttatgcat cgggacatcc
     4501 ttaccaagaa tttcggcaca gatcactgta tcctgtgcaa agtttttggg caccccgccc
     4561 ccgatcatga ataatccggt tgattcggcc gccatcttta ccagcgtcag ctccaggaaa
     4621 tccttcaccg aatcgatgct gacatgtttt ccgggatttt cccattgatg cttcacaagg
     4681 ccaaatccgg cgctgctgtc tgaaaaagcc gggcagaaaa tcggcacgtc atgttcgaaa
     4741 caggcctgga tgagagaatc tttcttaaca gagttctcgc taagatatcg gcccatctcc
     4801 cggataaatt cccttgaaga aaagggcctg ggctccagct tatctgcgat aagttttacc
     4861 gtcatatcgc aatgctgcaa ttcctcttca tcaatatagg tgtcgtaaat gcggtcgata
     4921 tagttattcc tcagcatccg gtcatcgata ctgggcgttc cccggtaatg tctgaacccc
     4981 agagcctcaa agaagtccat atcgactatg gaagcccccg tggccacaat ggcatcgatc
     5041 atattgtttt tcaccatatc tacatatacc tgcatacatc ctccggcact ggtgctgccg
     5101 gccagggtca gccagatcga acagctttta tcattgatca taagctgcag gatatcagct
     5161 gcattagccg tttcacgaga agtgaacgac atcttccgca tcgaatcgat cacaggggct
     5221 gaatcaaacg atttgatatc gacatgttca attgcctgtt tcaaaagatc tttcttttcc
     5281 atagtatcga gtttttatat ttaatcaaca tgaatagtat tattttaacc catccgcgat
     5341 aaccggatta accaaaattc cgcaggctga attacttatt gatttacaaa ttgatagctc
     5401 agcatctggt aaataagttt ggacgccaga aagtcagagg ctttgttatc aactatagga
     5461 cacaattcaa caacatcaaa accaactact ttgcgggtat ttatcagttt cctgacaagg
     5521 tgcaacacct catagtagtt taacccaccg ggctccggtg tgccggttgc aggtaacact
     5581 gatgggtcga atccatcgag atcgatagtg atatacactt gatcaccaag taattctata
     5641 accctgtcga tccagttatt cccggacccg gcgatatctt tggcataaaa gatcctgcct
     5701 ttatcgacaa aaggtttttc caccacatcc atactgcgga taccaacctg cacgatcggg
     5761 cacagttccc tgacacgcga catgacacag gcatgattaa aacgggagcc ttcgtattcg
     5821 gggcgaagat ctgtatgggc atcgatctgc aatacatgca gtcccggaaa tctctctgca
     5881 aatgcctgca cagcgccaat agttaccgaa tgttcccctc ccagggttac gacgaattta
     5941 ccatcatcca gaaatcggct gatggatttt ttcaccttaa cggccagatt ttccggggaa
     6001 ttttcttcga tgaccggttc agcggtaaaa attcccttta gataaacctc ggaatcggtc
     6061 tctatatcat aaagttccat atttgccgat gcttccagga tggcatctgg tcccctgtca
     6121 gcccccttga tccaggtact ggttgcatcg aacggaaccg gcaggataac aataggagac
     6181 acttcataac ctgacagttc agcgggtatt ccaccaaaat tcatatatac ggtttaaatt
     6241 agaaaactct aaaatacgac ttttttgatt acattagtaa cccagaacct tcatcatcgc
     6301 tttatagctt tgttccttgg caaacagctt ggtattgtat tccccatcgc tgtcgcggtc
     6361 gatgataata tgtttcggcg ccggaataag gcaatgctgt atgccaccgt aaccgccgat
     6421 cgattcctgg taggcgccgg tatgaaacaa accgatatac tgaggaattc ccggtctcat
     6481 tttaggtaag aagatggcat tggaatgagc ctcggagtta taataatcca tgctgtcgca
     6541 ggtcagtccg cccaggaata cgcgctcata ttcagcatcc cagttattga cggctaaaag
     6601 gatgaacctt tggtttagcg cccaggtatc cggaagtgtt gtcataaaag agctgtcgat
     6661 catattccag atctcccggt cgttctgcct tttctgatca atgatggaat agagcacggc
     6721 cccgctttcg ccgaccgtga aggaaccgaa ttcggtaaat atatcgggtt caggcacatt
     6781 attctgattg caaatgattt taatctgtga aatgatctcc tctgccatgt actcgtaatc
     6841 ataatcaaat gtaagagagt tctttacggg aaatccaccg ccgatgttca gcgaatcaag
     6901 ttcggggcat atttttttga gctcacagta aacattgacg catttcgaca gctcattcca
     6961 gtaataggct gtatcccgga taccggtatt gatgaagaag tgcagcattt tcaaggcaaa
     7021 cttgttgtta ttctgtattt tttcctggta ataggaaaca atatcattat accttatacc
     7081 aagcctggag gtataaaatt caaatttcgg ttcttcttcc gaagcaatcc tgatccctaa
     7141 ttttaccttt tttctaatcc cttttgaaaa tttatccagc tcgaaaatat tatcgaggac
     7201 cggaatagta ttttcaaagc cacggtttat cagattaata atgttttcaa tgtattgagg
     7261 gcgtttaaat ccattgcata ttatatgggt atccttattg atccggcctt cttcaaacaa
     7321 ctgctccaca atatgaatat caaaggctga agaggtctcg atatgaatat cgtttttcat
     7381 tgcttcctgc aggataaatg aaaagtgaga acttttcgtg cagtaacaat atatgtaatt
     7441 gccgttataa tcggctttgg ccatggccac gttaaaccat cttttagcct tctggatctg
     7501 ctcacttatc tttggcagat aactgatttt taacggagtg ccatattgtt ttataatgtc
     7561 catcagcgga atttcatgaa aattcagctc atattcaatc acttcgaact cattcgttgg
     7621 aaaatcgaag gtttgttcaa tcagatccag atacttaact ttcatatcaa tacaattaac
     7681 ttgttaaacg gcaggcaaag ataatatatt aaagatatca ctgtcaagaa acttcaatcc
     7741 aactcaccta aatcagtaat ttttactcca tccatcttag tcggattttt aattatctga
     7801 tattcttatg tttatacata atagaaatga tacaatttgt ccttccaatt ttcaattttc
     7861 aaatttccag ttgtattaca ttcttttacc aataaaaaat gaaggtacta atggaaatta
     7921 gaaataatat gctaattttg caccctccaa aaaacaggta taaaatcata aaattctgta
     7981 aacatgcaaa gtaagggagc tattaaggta tttgcgattg catttgcatt agtttgtctt
     8041 ttccagctat catttacatt tttcagcggc attacggagc ggaaagcgcg taaatatgcc
     8101 acaaacgctg aggcgatgag actggccgat gaattggcgg gcggcagtca gaggagtaaa
     8161 acctttgtga tggattcgct ggcaaaagcg cgagaatcgt attatctcga ttccatggcg
     8221 aatcaaccca tttacaatat tctcgttaaa gaatatactt ataaagaagt caaagaaagg
     8281 gaaatcaacc tggggttgga tctgcgaggt ggtatgaatg tcgtcctgga agtttcgata
     8341 ggagaaattg tcaaggcact ggccggaccc aatgctgtca acccgatctt ccagcaagcc
     8401 atggagcgga cctatgaaaa gcaaaaatcg agcgatcgcg attttgtaac tttattcgga
     8461 gaagcattcc gtgaagtcga tcccaatgcc aacatggctt ctatcttcag cacggtagaa
     8521 tttaaggata aaatcaccta taactccaca aatgatgaag ttcttgaggt tttaagagat
     8581 gaaaccgaag gggcctttga tatgacgtac aatattatcc gtactcgtat cgacaggttc
     8641 ggcgttaccc agccgaatgt ccagaaactg accggaaccg gccgaatcct ggttgagctc
     8701 ccgggcataa aagagcctgc ccgtgttcgt aaacttctcc aggggacagc caagcttgaa
     8761 ttctgggaga cctacaacta ctcagaagta tacggatatt ttgcagatgc caacagccgt
     8821 cttgtgacga tcctcggatc tcaaaacctg gaagacaatg gcacggctgg caagaccagg
     8881 gaaggagaaa cttcggatac cggagaaaac ggtagctccg ctactggcga cacaaccaaa
     8941 tccctccttg acaagataga gagcgacaca tcgaaagctg cccaggacaa gtcgtttgaa
     9001 gagtatgcca aagacaatcc cctgaatgct tacctgatgc cggccttttt ccagggtgaa
     9061 gacggccagt attacccggg acaaagcgcg acagtaggat ttgccgccat taaggatacg
     9121 gcgaaagtta atgatatgct caggaaagtc aatagcgtct tcccccggaa cctcaagctg
     9181 gtatggacgg ttaagcccag agatgccaac tccgatgtgc tcgaactggt agcgctgaag
     9241 gtgacctcac gcgacggcac cccggccctt ggcggtgaag ttatcgtaga tgcccgccag
     9301 gattacgacc agcttggcaa tgttgaggtc agcctgaata tgaatgctga aggtgcccgg
     9361 acatggaaac gcctcaccgg tgagaacata gataaacaga tcgctatagt gctcgacaac
     9421 cacgtttatt catttccgac agtaagaggt gaaatcccca acggtatgtc ttccatcagt
     9481 ggaggcggaa tgaccctgga agaagcacag gatctctcca atatcctcaa agccggtaag
     9541 ctccccgctt cggcacgcat cgtcgaagag gcggtcgtag gcccttca
//
LOCUS       DAOPNH010000121         9451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7918_length_9451_cov_6.128778, whole genome shotgun sequence.
ACCESSION   DAOPNH010000121 DAOPNH010000000
VERSION     DAOPNH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7918_length_9451_cov_6.128778"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKZ74_07740"
     CDS             <1..230
                     /locus_tag="PKZ74_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92689.1"
                     /translation="PFILSLYTEKEGSAMVHIYSVHGKEVYSHSIDLHRGDNEYFIDH
                     TGPGPGMYFIIVDKVTATADFHRFCGRIMVK"
     gene            457..1632
                     /locus_tag="PKZ74_07745"
     CDS             457..1632
                     /locus_tag="PKZ74_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPM92690.1"
                     /translation="MMITYEEALEIVLRNTYLSDKETVQIQEAIGRALADDVFSDINM
                     PPFNRSAVDGFACRRADHGQPLEVIETVRAGLFPQKKIGTGQCTRIMTGAPVPEGADC
                     VMMVEETEETADRRISFRGSQTKTNISLFAEDVREGDCVLKAGTMIAPHHIAIMAAVG
                     CVRPVVSKKPRVAVIATGDEIVEPHLKPGISQIRNSNGYQLTAQAIRSGAEANYMGIA
                     PDTEEDTFRILDNALDQADVILLTGGVSMGTFDFVPKILEKAGIEILFRTIALQPGKP
                     TVFGVKGDKRIFGLPGNPVSSFTIFEILVKPMLWKMMGITEPVRNMILTFGGHYQRKR
                     GDRMQWLPVKIKAGKVYPLEYHGSAHIFALADADAIAAIPLGITELNEGDLIDVRPV"
     gene            1616..2416
                     /locus_tag="PKZ74_07750"
     CDS             1616..2416
                     /locus_tag="PKZ74_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM92691.1"
                     /translation="MFDRFDRQINYLRISVTDRCNLRCTYCMPADGITLLRHEDILSY
                     DEIVEVAQIAAEMGVSKIRLTGGEPLVRKGITNLVKMLASIPGIVDLSMTTNGILLER
                     FAQELKDAGLQRVNISLDTLDAERFRKITRGGDIEEVKKGIIKAREVGLTPVKINCVT
                     GYEMDPEDAARVKEFSEANGFQVRFIRQMDLASGEFYVVDGGDGGNCNRCNRLRLTSN
                     GIVKPCLFNAIGYNVRELGAREAILQAVSNKPRCGTANSEEEFSWIGG"
     gene            2532..2984
                     /gene="moaC"
                     /locus_tag="PKZ74_07755"
     CDS             2532..2984
                     /gene="moaC"
                     /locus_tag="PKZ74_07755"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPM92692.1"
                     /translation="MGSSFSHTDESGRARMVDVGHKPEQLRIATATGYIRLNPETIRL
                     IRENEIKKGDVLTVAQIAGIQAGKKTADLIPLCHPLQITKIDVVTAIGDSGVTVTATA
                     KCVGQTGVEMEALTAASVALLTIYDMCKAVDKEMVIGEIRLVEKRKEE"
     gene            3113..3994
                     /locus_tag="PKZ74_07760"
     CDS             3113..3994
                     /locus_tag="PKZ74_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPM92693.1"
                     /translation="MKQPVDSICLDEWGVKNDAHAGPWNRQVSLLAKESVDKFSKAAG
                     REIRFGEFAENITTDGIILHETHPLDRFIIGETELEVTQIGKECHGDSCAIFREVGNC
                     VMPKEGIFCRVIRPGTVRPGDPIIFKENIYKAMVITLSDRASQGIYEDKSGPRTIAIL
                     NEFFTLQNWKCEITHHIIPDDENELKSLLQKAKSDKTDIVITTGGTGIGPRDITPEAV
                     RSVIDKEIPGIMELIRIKFGMEKPNALLSRSVAGVAGSTLIYALPGSVKAVNEYMAEI
                     SKTLRHSVFMLHGIDSH"
     gene            4162..4458
                     /locus_tag="PKZ74_07765"
     CDS             4162..4458
                     /locus_tag="PKZ74_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92694.1"
                     /translation="MIMLRILKKIFLNRYFITLVAFAVWMIFFDTNSLKRQHALNQRI
                     TEIREMKAFYASEIARNNTLIEELINNPEAIEKFGREKYLMKRDSEDIYLVTRE"
     gene            complement(4462..6948)
                     /gene="priA"
                     /locus_tag="PKZ74_07770"
     CDS             complement(4462..6948)
                     /gene="priA"
                     /locus_tag="PKZ74_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPM92695.1"
                     /translation="METKFANVLLPLPLPGYFTYHVPGDFREMIAPGKRVVVPFGKKK
                     IYTALVHEVLEENPSSFEPKSILSVIDDRPVVFPVQFRFWEWMASYYMCTPGEVMNAA
                     LPSGFKLASESMVCLNPEADLETADLNEKEIMIVEALLSQKKLPVNAIADLLDQRKII
                     PIIHTLIDKGVVVAEEKLEEKYKPLRQTFIRLGKEYEDEEKLKAIFDELSKRAFKQLQ
                     ALMVFLSLTREASVKAMDVRKSDLLKNKDVTQQALTALINKGILEYFEIEASRLAAST
                     ALNDPGDIALSLHQKRAFSDIGRIFAEKEVILLHGITSSGKTELYIKLIDQALNERKQ
                     VLYLLPEIALTSQIINRLQRYFGDNVGVYHSKYNDNERVEIWNHCLHHEKDFEKGRFQ
                     VILGARSALFLPYHNLGLIIVDEEHDSSYKQYDPAPRYHARDAAIMLARLHGAKTLLG
                     SATPAIESYFNASNGKYGLVEMTERFGGVSLPEIVLANVREETRNKTMQSHFTSALIN
                     RIRDALALKEQVILFQNRRGFSLHLTCDSCDWVPMCKNCDISLTYHKRENKIKCHYCG
                     YSEALPQECPQCKSTRILMKGFGTEKVEEELGAIFPEATIRRMDLDSTRTKYAHQRLI
                     SDFEERRTDILVGTQMVTKGLDFDNVSLVGILNADNMLNFPDFRSHERSFQLMEQVSG
                     RAGRKAKQGQVIIQSYNPQHAIIQHVVHHDYHAFYRSQLTDRHKFQYPPFNRLIILKV
                     KHRDYNVLNKAASDLAHDLRVDLGKRILGPEYPLVSKIQNWYIKHIMVKIERGNNIAE
                     MKKIILNRIENTGRIPAYRQVRVVIDVDPV"
     gene            complement(7000..7980)
                     /gene="pfkA"
                     /locus_tag="PKZ74_07775"
     CDS             complement(7000..7980)
                     /gene="pfkA"
                     /locus_tag="PKZ74_07775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM92696.1"
                     /translation="MPSIKRIGVLTSGGDAPGMNAAIRAVVRTGIYHGLEVYGIYKGY
                     SGLIQGLIEPLHSRSVSNIIQRGGTILKTARSEEFRTQEGRKKAHENLIKHNIDALVV
                     IGGDGTFTGAKIFQEEFNYPIVGLPGTIDNDLYGTDYTIGYDTAVNTVVDAVDKIRDT
                     ADSHDRLFIIEVMGRDAGFIALRSGIATGAEKILIPESVTYTDELIEKLRTDYANNKT
                     SGIIIVAEGDDLGGGFDVARVIKEHFPQYDTRVTILGHVQRGGSPSAFDRTMASAMGY
                     QAVLALKDGVRGVMVGSINRKVSYTPFEKAIKLHQSINEELLEIAEVLSH"
     gene            complement(8218..9381)
                     /locus_tag="PKZ74_07780"
     CDS             complement(8218..9381)
                     /locus_tag="PKZ74_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPM92697.1"
                     /translation="MEEQRIKMIAKTFEGFEEILARELRDLGAEDIDLIKRGIHFSGD
                     KKLLYMANYNCRTALRILVPLSSFRASTDAMLYDGVRAIPWEKFLDFRGKFAIDSVIS
                     YSTFTHSGYVSLKAKDAIADRFRDKFGKRPDVDNNDPDLRVNVHIFREECTVSIDSSG
                     ASLHLRGYRRAQTEAPINEVLAAGLILMSGWDGKTPFLDPMCGSGTILIEAALIAGRV
                     PPGYYRPLFGFERWKDFDRRLWDRVKRECEEAISEPTAPIIGYDRDEKAILASQANID
                     EAGVEQFVKVEQADFTTASPPWRSGFLLTNPPYGERIKVEDLKQFYKDIGDVLKQKYA
                     GYTAWLLGSNEEAMKFIGLRPSRRIRVMNGPLECRLAKFELYEGRKGDKTLGR"
BASE COUNT         2295 a         2347 c         2488 g         2321 t
ORIGIN      
        1 gccctttcat cctttcgctg tatactgaaa aagagggatc agcgatggtg catatttatt
       61 ctgttcacgg aaaagaagtg tattcacatt caatcgacct tcatagagga gataatgaat
      121 atttcattga tcacacaggg cccggtccgg gaatgtattt tattattgtc gataaagtaa
      181 cggccacagc tgatttccac cggttttgcg gaaggataat ggttaaatga agtattactc
      241 tgtgctactc tgtgtaaatc tgtatcactc cgtggtaaaa ttagtttttg ttacaccaca
      301 gagtacacag agaacacaga gttatcataa agagagttaa ctgcttatga tgaggcaggc
      361 taaaaatccc cgggaaccgg cttccgtttc agtcgtcaaa cttagccgta agtttatttg
      421 gcttaatatt cctactttta caggctcatt ttttttatga tgatcacata cgaagaggca
      481 ctggagatcg ttttaagaaa tacttatcta tctgataaag aaacagttca aattcaagag
      541 gcgattggaa gagcactggc agatgatgta ttctcagata tcaatatgcc cccgttcaac
      601 cggtcggccg tggatggatt tgcctgtcgc agagcagacc acggacaacc gctggaggtg
      661 atcgaaacgg tccgcgccgg cttgtttcca cagaaaaaga ttggcacggg gcagtgtaca
      721 cgcattatga ccggagcgcc tgtccctgaa ggggccgatt gcgtaatgat ggttgaagag
      781 acggaggaga cagccgatag acggatctcg ttccggggat cacaaaccaa aacaaatatc
      841 tccctgtttg cagaagatgt aagagaagga gattgtgttt taaaagccgg cacgatgatc
      901 gctccgcatc atatcgccat catggctgct gtaggctgtg ttcggcccgt agtttccaaa
      961 aaaccgcgag ttgcagtcat cgcaaccggc gacgaaatcg tcgaaccgca ccttaagccg
     1021 ggcatctccc agatccgcaa cagcaatggt tatcagctca ctgcccaggc cataagatct
     1081 ggcgctgaag caaactatat gggtattgcc cccgatacgg aagaagatac attcaggata
     1141 ctcgataacg ccctggatca ggccgatgtg atcctcctta ccggcggcgt ttcgatgggc
     1201 acatttgatt tcgtccccaa aattctcgaa aaagcaggca ttgaaatcct tttcaggacc
     1261 attgccttgc aacccggtaa accgaccgtt ttcggcgtaa aaggagataa gcgcattttc
     1321 ggattgcccg gaaatccggt ctcttcgttt actatttttg aaatcctggt gaagccgatg
     1381 ttatggaaaa tgatgggaat aacagaaccc gtcagaaata tgatactgac ttttggaggg
     1441 cattatcaac gaaagcgggg cgaccggatg caatggctcc cggtaaagat taaagctgga
     1501 aaggtttatc cgctggaata tcacggatcg gcacacatat ttgcactggc cgacgccgat
     1561 gccatcgccg ccatacctct cggaatcact gaactcaatg aaggagatct gatcgatgtt
     1621 cgaccggttt gaccgccaga tcaattacct gcgcatctcg gtcaccgacc ggtgcaacct
     1681 gcgctgcact tattgcatgc ctgccgatgg gatcacgctg cttcggcacg aagatatcct
     1741 gtcgtacgat gaaattgttg aagtagcgca aatagctgcg gaaatgggtg tcagcaagat
     1801 caggctgacc ggcggagaac cactggtgcg caaaggaata accaacctgg taaagatgct
     1861 ggcctcgatc cccgggatcg tcgatctgtc gatgaccacc aatggcatcc tacttgaaag
     1921 atttgcgcaa gagctgaaag atgccggtct tcagcgggtg aatatcagcc tcgatacgct
     1981 ggatgcggaa aggttcagaa aaatcacccg gggaggcgat attgaagagg tgaagaaggg
     2041 aattataaaa gccagggagg tcgggttaac cccggtcaag atcaactgcg tcacaggtta
     2101 tgagatggat ccggaggatg cagcccgggt gaaagaattc agcgaagcaa acggattcca
     2161 ggtccggttc atccgtcaga tggacctggc ttcgggtgaa ttttatgtgg tagacggagg
     2221 cgacggagga aactgcaacc gctgcaaccg tctccggctg acctccaacg ggatcgtcaa
     2281 gccgtgcctg ttcaacgcca tcggctacaa tgtcagggaa cttggggcaa gggaagctat
     2341 tttgcaggcc gtcagcaaca aaccccgctg cgggacagcc aattctgaag aggagttttc
     2401 gtggatcgga ggatgagata agactatagt cattagctca cttcgttcgc gtcattggct
     2461 tgctgcgcaa gcgtcattgg aaatatcgaa ttatctttga actttgaact ttgaactttg
     2521 aactttgaaa tatggggagc agcttttcgc ataccgatga atcgggccgg gcaaggatgg
     2581 ttgatgtggg ccacaagccg gaacagttaa ggatagctac agcaacgggc tatatccgcc
     2641 tgaacccgga gaccatccgg ctgatacggg aaaacgaaat aaaaaaaggc gatgtgctga
     2701 cggttgcgca gatcgccggt atccaggcag gaaaaaagac cgctgatctg atcccgctat
     2761 gccacccgct tcagatcaca aagatcgatg tcgtgacggc tatcggtgat tcgggtgtta
     2821 cagttactgc tacggcaaag tgtgtcggac agaccggcgt ggaaatggaa gcgctgaccg
     2881 ctgcatccgt tgccctcctt acaatttacg acatgtgcaa agcggttgat aaagagatgg
     2941 ttatcgggga gatcaggctg gtggagaaga ggaaggaaga gtgaagagtg aagggtgaag
     3001 agtgaagagt gaagagggaa aaaggaaaaa gaaatggtgc attttgaatc agaaatagga
     3061 aattggtatg attagggtat tgtcggttaa tatttcggaa aaaaagggga cggtgaagca
     3121 gccggtagat tctatctgcc tggatgaatg gggggtgaag aacgatgcgc atgccggtcc
     3181 gtggaaccgc caggtgagcc tgctggcaaa agagagcgtt gacaaattct caaaagccgc
     3241 cggacgggag atccggttcg gcgaatttgc cgaaaatatc acgaccgacg ggatcatcct
     3301 gcatgaaacc catcccctcg acaggttcat cattggcgaa accgaactgg aagtgaccca
     3361 gatcggcaaa gaatgccatg gcgatagttg cgccatcttc cgcgaggttg gcaattgcgt
     3421 gatgccgaaa gaaggtattt tctgccgcgt gatccgcccg ggaaccgttc gtccgggaga
     3481 cccgatcatc tttaaagaaa atatttataa ggcaatggtg atcaccctca gcgacagggc
     3541 cagccagggc atttatgaag ataaaagcgg gccgcgcacc atcgccattt tgaatgaatt
     3601 ttttacactt caaaactgga agtgcgaaat cactcatcat atcatccccg atgatgagaa
     3661 tgaactgaaa tcgttactgc aaaaagcaaa aagcgacaag accgacatcg ttattaccac
     3721 cggtggcacg gggatcgggc cgagggatat cacaccggag gcggtgcgtt cggtaattga
     3781 taaagagatt ccgggcatta tggaactgat ccgcatcaaa tttggtatgg aaaaaccgaa
     3841 tgcattgctg agccggagcg tggccggggt ggccggttca accctgatct atgctctccc
     3901 gggtagcgtt aaagcggtca atgaatatat ggctgaaatc tcaaaaacct tgaggcattc
     3961 agtttttatg ctacatggca ttgatagtca ctaatttatc acctgacatt ttcagtcgtc
     4021 ggaagtaaaa cgcgggcaat ggcgcttcaa cggattatag tgtttatatg gcatatcggt
     4081 tatcaacaaa atcaagttaa aaattcctgc tattttaagg aatcatctgc attatcaacc
     4141 ttatctttaa atcctgatta aatgatcatg ctccgcatcc tgaaaaaaat attcctcaac
     4201 cgctatttca tcacactggt agcattcgcc gtatggatga ttttcttcga tacgaacagc
     4261 ctgaagcggc aacacgcatt gaaccagcgc attaccgaaa tcagggagat gaaagcgttt
     4321 tatgcgtcgg aaattgccag gaacaacacc ttgatcgagg agctgatcaa taatcccgaa
     4381 gccattgaaa aatttggccg ggagaaatac ctgatgaaac gcgacagcga ggatatctac
     4441 ctggttacga gagagtgaag atcaaaccgg gtcaacatca ataaccaccc gtacctgcct
     4501 gtaagccggg atcctgccgg tattttcaat cctgtttaga ataattttct tcatctcagc
     4561 gatattgttc ccccgctcaa tcttgaccat gatatgcttg atataccagt tctggatttt
     4621 tgagaccagc gggtattcgg ggccgagaat cctttttccc aggtccaccc gcagatcgtg
     4681 ggccaggtcg ctggcagcct tgttcagcac attgtaatca cggtgcttga ccttcaggat
     4741 gatcagccgg ttgaaaggcg ggtactggaa tttgtgccgg tcggtgagct gtgagcggta
     4801 gaaggcgtga taatcgtgat ggacaacatg ctggatgatc gcatgttgcg ggttatagct
     4861 ctggataatg acctggcctt gcttcgcttt cctcccggcc cgcccgctga cctgctccat
     4921 gagctgaaaa ctccgttcgt gcgaccggaa gtctggaaaa ttaagcatgt tatcggcgtt
     4981 caggataccg acgaggctga cgttgtcgaa atccagccct ttggtcacca tctgcgtccc
     5041 gaccaggata tcggtccgcc gctcttcaaa atcggagatc agccgctggt gggcgtattt
     5101 cgtccgcgtg gaatcgaggt ccatacggcg gatggtggct tccgggaata tcgcgccaag
     5161 ttcttcttcc actttctccg ttccgaaccc tttcatcagg atacgcgtgc ttttgcactg
     5221 cgggcactcc tgcggcaatg cttccgaata accgcagtag tggcatttga tcttgttctc
     5281 ccgcttatgg taggtgaggg agatgtcgca gttcttgcac atcgggaccc agtcgcacga
     5341 atcgcaggtc aggtgcagcg agaatccgcg ccggttctgg aaaaggatca cctgctcttt
     5401 cagcgccagc gcatccctga tgcgattgat gagggccgag gtaaagtgcg actgcatcgt
     5461 tttattcctc gtctcctccc tcacgttggc cagcacaatc tccggcaggc tgaccccgcc
     5521 gaaccgttcc gtcatctcca ccagcccgta ttttccattc gaagcattaa agtagctttc
     5581 aattgccggg gtagcagagc ccagcaatgt ttttgcgccg tggagcctgg ccagcatgat
     5641 cgccgcatcc cgggcatggt acctcggcgc cggatcatat tgcttgtacg atgaatcgtg
     5701 ttcttcatca acaatgatta ggcccaggtt gtgatagggc aggaacaggg cggagcgggc
     5761 gcccaggatc acctggaacc ggcccttctc aaaatccttt tcatggtgaa ggcagtggtt
     5821 ccagatttct acccgttcat tgtcgttata ttttgaatga taaacgccta cattgtcccc
     5881 gaaatagcgc tgcagccggt tgatgatctg cgatgtcagc gcgatctcgg gcagcaggta
     5941 cagcacctgt tttctctcat tcaacgcctg gtcgatcagt ttgatataca actccgtctt
     6001 tccgcttgat gtgatcccat gaagaaggat cacctctttc tctgcaaaaa tgcgcccgat
     6061 atcgctaaaa gcccttttct gatgaaggct cagcgcaata tcgcccggat cgttcaacgc
     6121 ggttgaagcg gccagacggc tcgcctcgat ctcgaaatat tccagtattc ccttgttaat
     6181 caatgcggta agcgcctgct gcgtcacatc cttgtttttc aggaggtcgc tttttctcac
     6241 atccatcgct ttaacggaag cttcccgggt cagcgacagg aaaaccatca gtgcctgcaa
     6301 ttgcttgaat gcccgcttgc tgagttcgtc aaatatggct ttgagctttt cttcatcttc
     6361 atattctttt cccagccgga tgaaggtctg cctgagcggc ttgtacttct cttcgagctt
     6421 ttcctcggcg acgacgaccc ctttatcaat taatgtatgg atgatcggga tgatcttccg
     6481 ctggtcaagc aggtcggcaa tggcatttac aggaagtttc ttctgggaga gcaacgcttc
     6541 cacgatcatg atctctttct cgttgagatc ggcggtttcg aggtcggctt cggggttcag
     6601 gcagaccatc gattcgcttg ccagcttgaa cccggaaggg agggcggcgt tcatcacttc
     6661 gccgggggta cacatgtagt acgatgccat ccattcccag aaccggaact ggaccgggaa
     6721 aacaacgggc ctgtcgtcga tgaccgacag gatgcttttc ggttcaaaag aggaggggtt
     6781 ttcttccagg acttcatgga ccagcgccgt atagatcttt tttttaccga atggcaccac
     6841 gacccgcttg cccggcgcaa tcatttcccg gaaatcaccc gggacatggt aggtgaaata
     6901 gcccggcaaa ggcaggggca gaaggacatt tgcgaatttg gtttccaacg gattcagcgc
     6961 ttgatttaaa ggctgaaagg tacgaaataa ccggtcaggt taatgcgaaa ggacctcggc
     7021 aatttccaac aattcctcgt tgatactctg gtgcagcttg attgctttct caaacggcgt
     7081 atagctgact ttccggttta tcgagccgac catcacgccg cgcaccccgt ctttcagggc
     7141 cagcaccgcc tggtatccca tcgcgctggc catggtgcgg tcgaaagctg agggcgaacc
     7201 gccgcgctgg acatggccca ggatcgtgac ccgggtgtcg tattgcggaa aatgttcctt
     7261 gatcaccctg gccacatcga atccgccgcc caggtcgtcg ccttcggcta cgatgatgat
     7321 ccccgaggtc ttgttgttgg catagtctgt ccgtaatttt tcgatcagct catcggtgta
     7381 ggtgacactt tccgggatga ggatcttctc ggcgccggta gcgatgccgc ttcttaatgc
     7441 gatgaatccc gcatcgcgcc ccatcacctc gatgatgaac agcctgtcgt gtgagtcagc
     7501 cgtatcacgg atcttatcta ctgcatccac cactgtgttg acggccgtat cgtaaccgat
     7561 agtgtagtcg gtgccgtaga ggtcgttgtc gatcgttccg ggcagaccga cgatcggata
     7621 gttgaattct tcctggaaga ttttcgcccc ggtaaaggtg ccgtcgccac cgataacgac
     7681 caacgcgtcg atattgtgtt tgataaggtt ctcgtgggct tttttgcggc cttcctgggt
     7741 tctgaactcc tcgctgcggg ccgtcttcag aatggttccg ccacgctgga tgatattgga
     7801 gaccgaccgg ctgtgcaagg gttcgatcaa tccctggatg agcccgctat agcctttata
     7861 gattccgtaa acttcgaggc cgtgatagat tccggtgcgc accacggcgc ggatggcggc
     7921 attcatcccc ggtgcatcgc cccccgaggt cagtacgcct attcttttaa ttgatggcat
     7981 tttttcgttg atctgatttt cggtgcaaag gtactgctaa aaggcaacaa gaatgaagat
     8041 gtttaaaaag tcctttgtgg agcttcgtga aaccttcgtg gaacttcgtg taatttataa
     8101 atgattgttg cacagagatc cacgaagaag gcacgaaggt acacgaagct attttgaaag
     8161 aaatcttacc ttttgaaaca cgcttttatt aatactttat cctgcctttc tgcagtccta
     8221 ccgtcctaaa gtcttatctc cctttctccc ttcgtacagc tcaaacttcg ccagccggca
     8281 ctccagcggg ccgttcatca ccctgatccg gcgggaaggg cggaggccga tgaatttcat
     8341 ggcttcttcg ttggaaccca gcagccacgc ggtatagccg gcatatttct gtttcagcac
     8401 gtcgccgatg tctttgtaaa actgtttcag gtcttccacc tttatccgct cgccgtaagg
     8461 tggattggtg aggaggaatc cgctgcgcca gggcggggaa gcggtggtga aatcggcctg
     8521 ctcaaccttg acgaattgct ctacgccggc ttcatcgatg ttggcctggc tggccaggat
     8581 ggctttttca tcacggtcgt agccgatgat aggtgcagtg ggctccgaaa tcgcctcttc
     8641 gcattcgcgc ttcacccggt cccatagcct ccggtcgaaa tccttccatc gttcaaagcc
     8701 aaacagcggc cggtaataac cgggaggcac cctgccggct atcagcgccg cttcgatcag
     8761 gatcgtccct gaaccgcaca tgggatcaag aaagggcgtt ttgccgtccc atccgctcat
     8821 caggatcagg ccggcggcca gcacctcgtt tatcggcgcc tcggtctggg cccggcgata
     8881 acccctcaga tggagcgaag cgcctgagga gtcgatggat acggtgcatt cttcacggaa
     8941 aatatgcaca ttgacccgca gatcgggatc gttgttatcg acatcgggac gtttgccgaa
     9001 tttgtcgcgg aaccggtcgg caatcgcatc tttcgctttc agcgacacat agcccgaatg
     9061 tgtgaatgtg gagtagctga tcacgctgtc gatggcgaat ttcccccgga aatcgaggaa
     9121 cttttcccac gggatggccc tgacgccgtc gtaaagcatg gcatcagtgg atgccctgaa
     9181 gcttgaaaga ggtaccagga tgcggagcgc cgtccggcag ttgtagttgg ccatgtacag
     9241 caacttctta tcaccgctga aatgaattcc ccgtttgatc aggtcaatat cttccgcgcc
     9301 aaggtccctc agctccctcg ccagtatctc ctcaaaacct tcaaaggtct tggcaatcat
     9361 ttttatgcgt tgttcttcca tgccggatcg ggtttggtag tccgacgggt aaatccgcgg
     9421 gttccaaagt tagtgtaaaa atattttttt a
//
LOCUS       DAOPNH010000122         9430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7949_length_9430_cov_7.152960, whole genome shotgun sequence.
ACCESSION   DAOPNH010000122 DAOPNH010000000
VERSION     DAOPNH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_9430_cov_7.152960"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKZ74_07785"
     CDS             complement(<1..426)
                     /locus_tag="PKZ74_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPM92698.1"
                     /translation="MKFQPQQYNLPDERMKPFIDPDEISSLLARSFPTREQVREVLAK
                     ALAKNRLSMEETAILVNATDPQLVEEIKAAARKLKKDVYGERIVLFAPLYVGNYCVNN
                     CKYCGFAASNRKALRKTLTNEELVRNVEALEENGQKRLIL"
     gene            complement(510..1637)
                     /locus_tag="PKZ74_07790"
     CDS             complement(510..1637)
                     /locus_tag="PKZ74_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPM92699.1"
                     /translation="MATLKVLVVDDEPGIRSGIIRIMRNFRVGYPFMDEDFDFEVIEA
                     ETGERAIEIIEAQPMDIILLDNKLPGMDGIEVLDYIKKKEYDAQVMMITSYASLDLAI
                     KATNTGAYNFMPKPFTPDELKSSIEGITKHLYLKRMTRNMNREGKQVRFQFLSVLSHE
                     LKSPINAVEGYLKIMKERQVGDNLADYDTMIDRSLERIKGMRNLIMDLMDLTRLESGK
                     KVREMRKVDLNQVARIAMDTMEPMAIQKNVKMYLDAPENLTMVGDPDEIEIIVNNLVS
                     NAVKYNREGGSVNVMMRQSSSSLNITVEDTGIGMSEDDIKLLFQEFVRIKNEKTKLIT
                     GSGLGLSIVKKLVEETYKGTINVKSQPDVGSTFVVVLPLIL"
     gene            complement(1713..2108)
                     /locus_tag="PKZ74_07795"
     CDS             complement(1713..2108)
                     /locus_tag="PKZ74_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM92700.1"
                     /translation="MKESKKTILIVDDDIDYLSQMELQVKRLGYDVVTADTQKEGEEL
                     IGKGKFDLAIIDLMMENKDSGFILCYKMKQKNPAMPVIIASAVTAETGLSFGVETAQD
                     RSWIKADTFIEKGIRPDQLEREINKLLKI"
     gene            complement(2192..4171)
                     /locus_tag="PKZ74_07800"
     CDS             complement(2192..4171)
                     /locus_tag="PKZ74_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM92701.1"
                     /translation="MAHSKKYAKLVYTVKDRCRVCYTCVRECPVKAIRIFNGQAEVIA
                     ERCIACGNCVRVCSQDAKVFIDSREQVKIFLKGDRPVYAVIAPSFPAEFREIRDYRVL
                     VGMIKKLGFEKVVEVSFGADMVAMEYNRLLGGNGGKHYISSDCPAIVNYVENYHPDLV
                     PSLAPIVSPMVALCRVIRKKYGEEPLIVFIGPCIAKKAESDEIDEAITFRELRKLFQE
                     FNVTPEQTQPAEFDEPVSGKGAIFPVSRGLLQTANKNDDLVEGNIMVADGRVNFREAI
                     KEFENGLMNSQHLELLCCEGCIMGPGMTYGCKKFEGMALVSEYVREKMQNLDEEKWEN
                     QVEEFSGIDFSQQFSRKERTPLQAPKEKVDEVLFKMGKVSEKDQLNCGACGYDTCVDH
                     AIAIVKGLAENEMCLPYAIEKLHTSLKDLNKSNEQLATTQQALRQSEKLASMGQLSAG
                     IAHELNNPLGVITMYTNILKDETPPDSPVYKDLQLIVEQAERCRGIVGGLLNFARKNQ
                     VFLVETNTVDFVKRSVDSIIRPDNIDIKIENKLSDPIARIDKDQMMQVLTNLEKNAVE
                     AMPKGGTLKISLTGTDHEVELAISDTGIGIPEENMEKIFTPFFTTKEVGKGTGLGLPL
                     IYGIVKMHRGQVQVTSNADPWKGPTGTTFTIKIPR"
     gene            complement(4246..5973)
                     /locus_tag="PKZ74_07805"
     CDS             complement(4246..5973)
                     /locus_tag="PKZ74_07805"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPM92702.1"
                     /translation="MDFQIEVNNKKLDAKQGETILDVLNRNGMKVPTLCHMKDRIPTG
                     SCRMCVVELENNGRLVPSCSFPASPEMIIKTHSPRVVDARKTIVELLLSNHPDDCLYC
                     VRNENCELQSLSREHHVTERRISGLKNNFSLDRSSASLVRDPDKCILCGRCVRVCEEV
                     IGVSAIDFIGRGSKTHIGTAFDGGLNTSSCINCGQCILVCPTGALHEKSHFGEVLDAL
                     NNPNKIVVVQYAPAISVSLAEAFHMKTGEDINGLLNAVLRKIGFDFVFDTTFSADLTI
                     MEESAELIHRIVNGEKMPMFTSCCPAWVKYVEEFAPEFLPNVSSCKSPQQMLGAVIKT
                     YFADIEGIKPEDIYSVSIMPCTAKKFEAQRTSMTRKGLTDVDAVLTTRELVDLIRMYG
                     IQIEQLEPEGADSPMGARSSAGKLFGASGGVMEAALRTAYFKLTGKEMVQFRVNEVRG
                     LNGRKETKVQIGDLVLGIAVVSGMANAKPLLDEIRNGRDDIHFVEMMACPGGCIAGGG
                     QRIGAGLEAIRARMKSLYDIDDRESIKVSHRNPEIQEIYKNFLGEPLGHKSHELLHTK
                     YIERDDVLL"
     gene            complement(5995..7905)
                     /locus_tag="PKZ74_07810"
     CDS             complement(5995..7905)
                     /locus_tag="PKZ74_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM92703.1"
                     /translation="MGFLVENPSDETSGIREKQLKVLRREDVSAPVIFIGTGTCGLGA
                     GAGKTKLAAQEYLTRQGIDATIIDVGCIGLCSSEPILDVQLPGMKRISFEKVSADKVE
                     GILDKVFYKELPEHPVLGQFNQGDGKNWEGVPALDEHPFFAPQTRVVLQSCGIIDPES
                     IEEYIAHGGYQTLVKTLQTKTAEEVCRFAEESGLRGRGGGGFPTGKKWSLANQEKADQ
                     KYLICNADEGDPGAFMDRAVIEGDPHRLIEGMSIAAYGIGASKGYVYIRAEYPLAVKR
                     LKNAITQAYDLGLLGSNIAGTNFSFDLLVKMGAGAFVCGEETALIHSIEGKRGMPRPR
                     PPFPSTSGLFGKPTVINNVETLSNVPTIFQKGEGWYSSIGTSNSKGTKVFALSGKINL
                     TGLVEIPMGTTIRDIIFKIGGGIRNGKKFKAVQIGGPSGGCITEENLDIQIEYESLLK
                     VGAMMGSGGLVVMDEDTCMVDVAKFFMDFIQRESCGKCIPCREGTRRMLEILESITRR
                     PDAGSSHDALDRFKGVMQLEKLGKIIKETSLCGLGQSAPNPVMSTLRWFRDEYEAHVF
                     DRVCPSGACTDLRVFVIDVDKCTGCTVCSKKCPTSAIIGAKKTPHFIVEDKCIGCGSC
                     YEACKFDAIYIK"
     gene            complement(8028..8486)
                     /gene="nuoE"
                     /locus_tag="PKZ74_07815"
     CDS             complement(8028..8486)
                     /gene="nuoE"
                     /locus_tag="PKZ74_07815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPM92704.1"
                     /translation="MSADINQIITEYSGGSENLIPILQKIQDREGFLSRHAVIEVGKK
                     LDIPASKIYGVATFYNQFRFEALGKYHVQVCRGTACHVLGSATVLQELEKMLRIKAGQ
                     TTRDGLFSMEVVACIGACGLAPVICINGEFHAKVTKESLQGIIDEYRNRE"
     gene            complement(8532..9044)
                     /locus_tag="PKZ74_07820"
     CDS             complement(8532..9044)
                     /locus_tag="PKZ74_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM92705.1"
                     /translation="METKKILIVDDDIDIITVVKAILSKEGYTVISASDKVEGFKLAY
                     KEKPDLAILDVMMTTQYEGFELAREFSENPEFANMPVLMMTSIDVLTTTKSAVQEMAR
                     EFRQDPSYRELDVLLVRDVITGKAGVDYRTEKGPSVWLPVDGFLRKPVDAKKLVPAVQ
                     HLMEIHAKAN"
BASE COUNT         2058 a         2441 c         2258 g         2673 t
ORIGIN      
        1 caggatgagc cgcttctggc cgttttcctc gagggcttcg acgtttctga ccagttcttc
       61 gttggttaaa gtcttcctga gcgctttccg gttggaagcc gcaaagccgc agtatttgca
      121 gttatttaca caataattcc cgacgtaaag cggagcgaaa aggacaattc gttcaccata
      181 aacgtctttt ttaagtttcc tggcagcagc tttaatctct tcaacaagtt gaggatcagt
      241 ggcattgacc aggattgcgg tttcctccat ggagaggcgg ttcttggcca gggcttttgc
      301 gagaacttcc ctgacctgct cccgggttgg aaaagagcgg gcgagcagag aactgatttc
      361 atcgggatcg atgaaaggct tcatccgctc gtccgggaga ttatattgtt gaggttggaa
      421 tttcatttta ttctatttat agcacgcgga tgacgcggat gcgacggatg accacggatt
      481 attattattc actattcatt attctttatt cacaggatta gtggcagcac aaccacaaaa
      541 gtgctgccca catccggctg gcttttgacg ttaattgtgc ctttgtatgt ttcttccacc
      601 agtttcttca cgatcgatag gccaagtccg ctgccggtga tcagtttcgt tttttcattt
      661 ttaattctta caaactcctg gaaaagaagc ttgatgtcat cttcgctcat tccaattcct
      721 gtatcttcta cagtaatatt taaagatgat gaggattgac gcatcatgac attgaccgaa
      781 cctccttccc gattatactt aacggcattc gacaccaggt tgttcacgat gatctcaatt
      841 tcatcgggat cgcccaccat cgttaaattc tccggggcgt cgaggtacat ctttacattt
      901 ttttgaatcg ccatcggctc catggtgtcc atcgcgatcc tggccacctg gttgaggtcg
      961 acttttctca tctcacgcac tttctttccc gattccagcc tggtcaggtc catcaggtcc
     1021 atgatcaggt ttcgcatccc tttgatccgc tccagcgaac ggtcgatcat ggtgtcgtaa
     1081 tcggcaaggt tatcgcccac ctggcgctcc ttcatgatct tcagataacc ttccacggcg
     1141 ttgatcggtg atttgagctc atgcgacagc accgagagga actggaacct cacctgcttg
     1201 ccttcacggt tcatattccg ggtcatccgt ttcaggtaga gatgcttggt gatcccttcg
     1261 atcgacgatt tgagctcatc cggcgtaaac ggcttcggca tgaaattgta tgctcctgtg
     1321 ttcgtggcct ttatagccag atcaagtgag gcataggagg tgatcatcat cacctgggca
     1381 tcatattcct tctttttgat ataatccagc acttcgatgc cgtccatgcc cggcaactta
     1441 ttgtcgagca ggataatatc catcggttgg gcctcgatga tctcgatagc cctttccccg
     1501 gtttcggctt cgatcacttc aaaatcgaag tcctcatcca taaacggata ccctaccctg
     1561 aaattccgca tgatccggat tatccccgaa cggatgccgg gttcgtcgtc gactacaaga
     1621 acttttagag ttgccattga gttagtcatt ttaaccctcc ccctttagtt ccccctccct
     1681 gatatcaggg agggggatag ggggtgggtt catcatatct tcaacagttt atttatctcc
     1741 cgttccaact ggtccgggcg gattcctttt tcgataaatg tatcggcctt gatccaggaa
     1801 cggtcctgtg ccgtttcgac gccaaagctc aggccggtct cggccgtgac ggccgaagca
     1861 atgatgaccg gcatggccgg atttttttgt ttcatcttgt aacacaggat aaatccgctg
     1921 tctttgttct ccatcatcag atcgatgata gccaggtcaa attttccttt accgatcagt
     1981 tcttcgcctt ccttctgagt atctgccgtc accacatcgt atcccagcct tttaacctgc
     2041 agttccatct gtgataaata atcgatgtcg tcatccacga tcaatattgt cttcttactt
     2101 tctttcattt ctctttacta ttttgattaa atcttttatt tccctccaca tgccccctcc
     2161 ccgttttggg gagggcattg gggtggggct tttatcttgg tatcttaatt gtaaatgtcg
     2221 ttcctgtcgg tcctttccac gggtcggcat tggatgtcac ctggacctgg ccacggtgca
     2281 tcttcacgat tccgtatatg agcggaagtc cgagccctgt tccttttcct acttccttgg
     2341 tagtgaagaa cggggtaaat atcttttcca tgttttcctc cgggattccg ataccggtat
     2401 cactgatagc gagctccacc tcatgatctg tgccggtgag cgagatcttc agggtgccgc
     2461 ctttcggcat ggcctccacc gcattttttt ccaggttggt cagcacctgc atcatctggt
     2521 ccttgtcaat ccgggcaatc ggatcgctga gcttattttc gatcttaatg tcgatgttat
     2581 ccggccggat gatcgaatcg acactccttt tcacgaagtc gaccgtattt gtttcgacca
     2641 ggaaaacctg gttcttacgg gcgaaattca gcaacccgcc gacaattccg cggcagcgtt
     2701 ccgcctgctc cacgatcagt tgcaggtcct tgtatacagg gctgtcgggc ggcgtttcat
     2761 cctttaaaat attcgtgtac atcgtaataa cgcccaaagg attgttgagt tcgtgggcga
     2821 taccggccga aagctgtccc atgctggcca gcttctccga ctggcggagc gcttgttggg
     2881 ttgtggccag ttgctcgttc gatttattca gatcttttaa tgaagtatgg agcttctcga
     2941 tagcataggg caggcacatt tcgttctctg ccaggccctt cacgatagcg atggcatgat
     3001 ctacacaggt atcgtatccg caggcgccgc aattaagctg atctttctcc gaaactttac
     3061 ccatcttgaa caggacttca tcgactttct ctttgggcgc ttgtagcgga gtccgctctt
     3121 tccggctgaa ttgctgagaa aaatctattc cggagaattc ctcaacctgg ttttcccatt
     3181 tctcttcatc caggttttgc attttctccc tgacatattc actgaccagg gccatccctt
     3241 caaacttctt gcatccataa gtcatcccgg ggcccataat gcatccttca cagcacaaca
     3301 gttccaggtg ctgggagttc atcagcccgt tttcaaattc tttgatggct tcccggaagt
     3361 taacccttcc atcggccacc ataatattgc cttcaacgag gtcgtcgttt ttattcgctg
     3421 tttgcagcag gccacggctt accgggaaga tagcaccttt gcccgaaacc ggctcatcga
     3481 attccgccgg ttgagtttgc tccggagtaa cgttaaactc ctggaagagc ttcctcagtt
     3541 ccctgaaggt gatcgcttcg tcgatctcat cagattctgc tttcttggcg atacaggggc
     3601 cgatgaagac aatcagcggc tcttcgccgt actttttgcg gatcacgcgg cacaaagcca
     3661 ccatgggtga tacgatcggc gccagcgacg ggaccaggtc ggggtgataa ttttcgacat
     3721 aattgacgat ggccgggcag tcggaggaga tataatgctt cccgccgttt ccacccagca
     3781 acctgttgta ttccatggcc accatatcgg cgccgaagga tacttccacg actttttcaa
     3841 atcccagctt tttaatcatg ccgaccagta cccggtaatc tctgatttcc ctgaattcgg
     3901 cagggaaact gggggcgatg accgcgtaaa ccggccggtc gcctttcagg aagatcttca
     3961 cctgttccct ggaatcgatg aatactttgg catcctggct gcaaaccctg acacaattcc
     4021 cgcaggcgat gcacctttcg gcgatgacct ccgcctggcc gttaaaaata cggatggctt
     4081 tcaccgggca ctccctgacg caggtgtagc aaaccctgca ccggtctttt accgtgtaaa
     4141 ccagctttgc gtattttttt gaatgtgcca tttgtcattt tttaaccctc ccccttgttc
     4201 cccctccctg ccagcaggga gggggatagg gggtgggttc cttaattata gcaatacatc
     4261 atctctctca atatactttg tatgcagcag ctcatggctt ttatggccga ggggttcgcc
     4321 gaggaaattc ttatagatct cctggatttc gggattgcga tgcgaaacct tgatcgattc
     4381 cctgtcgtcg atatcgtaca ggctcttcat ccgggcgcgg atggcttcga gacctgcccc
     4441 gatcctctgg ccgccaccgg cgatgcatcc gcccggacaa gccatcatct ctacaaaatg
     4501 gatatcgtcg cggccattcc ggatttcgtc gagaagcggc ttggcattgg ccatgccact
     4561 gacgacggcg atgccaagta ccaggtcgcc gatctggact ttggtctctt tccggccgtt
     4621 aagtccgcga acctcattca ccctgaactg caccatctct tttccggtga gtttaaagta
     4681 tgctgtcctt aaggcggctt ccatgacacc gcctgaagcg ccgaacagct taccggccga
     4741 actgcgggct cccatcgggg agtcggcgcc ttccggctcc agttgttcga tctggatgcc
     4801 gtacatccgg atcaggtcga ccagttcgcg ggttgtaaga accgcatcga catcggtaag
     4861 ccccttacgt gtcatagagg tacgctgggc ttcaaatttc tttgccgtac agggcataat
     4921 cgatacggaa tagatatctt caggcttaat gccttctata tctgcaaaat aggttttgat
     4981 gacggctccg agcatttgtt gcggggattt gcaggaggag acgttgggaa gaaattcggg
     5041 ggcgaactcc tccacgtatt ttacccaggc cgggcagcag gaggtaaaca tcggcatttt
     5101 ttcgccgttt acgatccggt ggatcagttc ggccgattct tccatgatcg taaggtcggc
     5161 agagaaagtc gtatcaaaca caaagtcaaa tccgattttg cgcagcaccg cattcagcag
     5221 gccgtttatg tcttctcccg tcttcatgtg gaatgcttcc gccagggaaa cggagatggc
     5281 aggcgcgtac tgaacgacca ctattttgtt ggggttgttg agggcgtcga ggacttcgcc
     5341 gaagtggctc ttttcatgca gggctccggt agggcagacc aggatgcact gaccgcagtt
     5401 gatgcagctt gaggtgttga gtccaccgtc gaaagcggta ccgatgtggg ttttcgaacc
     5461 acgtccgatg aaatcgatgg cggaaacgcc gatcacctct tcgcaaaccc tgacacatcg
     5521 gccacaaagg atacatttgt cgggatcgcg caccaggctg gcgctggaac gatccaggct
     5581 gaagttgttc ttcaggccgc tgatgcggcg ttcggtcaca tgatgctcgc gcgataggct
     5641 ctgcaattca cagttctcat tcctgacaca gtaaaggcaa tcgtcggggt ggtttgaaag
     5701 gagcagttca acgatcgttt tcctggcatc cacaaccctg ggtgagtgtg tcttaataat
     5761 catttcgggg gaagcgggga atgaacagga aggcaccaga cgtccgttgt tttcaagttc
     5821 gaccacgcac atccggcatg aaccggtcgg aatcctgtct ttcatgtggc ataacgtggg
     5881 cactttcatc ccgttgcggt tcagcacatc gaggatggtt tccccctgtt ttgcatccag
     5941 ctttttatta ttgacttcaa tctgaaaatc catgattata taatgatttt ggtgttattt
     6001 aatgtagatg gcgtcgaatt tgcaggcttc ataacagctt ccgcaaccga tgcatttgtc
     6061 ttcgacaata aaatgaggag ttttcttcgc tccgatgatg gctgatgtcg ggcacttttt
     6121 ggaacaaacg gtacatcctg tacatttatc cacatcgatc acaaaaaccc tgaggtcggt
     6181 gcaggcgccg gaagggcata cgcggtcaaa aacatgggct tcgtattcat cacggaacca
     6241 gcggagtgta ctcattaccg ggttgggtgc cgactggccg aggccacaaa gggaggtttc
     6301 cttgatgatc tttcccagtt tttccagttg cattacgcct ttgaaacgat cgagggcgtc
     6361 atggctgcta ccggcatccg gcctgcgggt aatgctttcg aggatttcca gcatcctccg
     6421 ggtcccttcc cggcagggga tgcactttcc gcagctttcc cgctggatga agtccataaa
     6481 gaatttggcc acatccacca tgcaggtgtc ttcgtccatg accaccagtc caccggaacc
     6541 catcatagcg ccgactttca gcaacgattc atattcgatc tgtatgtcga gattttcctc
     6601 ggtgatacac ccgcccgaag ggccgccgat ctggactgcc ttaaattttt tgccattccg
     6661 gatacctccg ccgatcttga agataatatc gcggatggtc gttcccatgg gaatttcgac
     6721 cagtcctgtg aggttgattt taccggaaag ggcaaacacc ttggtccctt tgctattact
     6781 tgttccaatg gagctgtacc agccttcacc cttttgaaag attgtcggaa cattggatag
     6841 tgtttcgacg ttgttgatga cggtcggttt gccgaacagc ccgctggtgg aaggaaaagg
     6901 aggccggggg cgcggcattc ccctctttcc ttcaatgcta tggatcaggg cggtttcctc
     6961 tccacacaca aatgcaccgg ctcccatttt gaccaataga tcaaatgaga agttcgtgcc
     7021 agctatgttg gaacccaata accctaaatc ataagcttgc gtgattgcat ttttaagccg
     7081 tttgacagcc aggggatact ccgcccggat gtaaacatag cctttggagg cgccgatccc
     7141 gtatgccgcg atcgacatgc cttcaataag ccggtgcgga tcgccttcaa tcaccgcccg
     7201 atccataaat gctccggggt ctccttcatc ggcattacag atgaggtatt tctggtcagc
     7261 cttttcctgg ttggccaggg accatttctt accggtcggg aaaccgccgc cgccgcgccc
     7321 tctgagcccg ctttcctctg cgaaacggca aacctcttca gcagtttttg tttgcagagt
     7381 tttaacgagt gtctgataac cgccatgagc gatatattct tcgatacttt ccggatcgat
     7441 aatgccgcag ctttgcagca caacgcgtgt ctggggggca aagaaagggt gctcgtccag
     7501 tgcagggacg ccctcccaat tcttgccatc gccttgatta aactgcccga gtaccggatg
     7561 ttcgggcaat tccttataaa atactttatc gagaatgcct tccactttat cggcggagac
     7621 tttttcgaaa gagatccttt tcattccggg caactggaca tccaggatgg gctcggagga
     7681 gcagaggcca atgcatccaa catcgataat agttgcgtca attccctgac gcgtcagata
     7741 ttcctgagct gcaagtttag tttttccagc ccctgctcca agcccacagg tacctgttcc
     7801 gataaagata acaggcgccg aaacatcctc cctccgtaaa accttcaact gcttttccct
     7861 tatcccggag gtttcatcgg agggattctc aaccaaaaaa cccattatga aaggattata
     7921 ttttaagttc ttttccattt ttaaaatctt aactgatcca atttacctac ccaatcaatt
     7981 tcttagtctt cactattccc ttttcactat tcccttttta ctattcacta ttccctgttc
     8041 ctgtactcat cgatgatccc ctgcagcgac tctttggtca ctttggcatg gaattcaccg
     8101 ttgatacaaa taacgggggc cagtccgcat gcgccgatgc aggcgactac ttccatgctg
     8161 aagaggccat cgcgtgtggt ttgcccggct ttgatcctga gcatcttttc cagttcctgc
     8221 agcactgtcg ctgaccccag gacatggcag gcggtgccgc ggcaaacctg gacatgatat
     8281 ttgccgaggg cttcaaaacg gaattgatta tagaaggtag ctacgccgta aattttactg
     8341 gccggaatgt cgagcttctt cccgacttcg ataactgcat ggcgggatag aaacccttcc
     8401 cggtcctgga ttttttgcag gatcgggatc aggttttcac tgccaccgct gtattcggtt
     8461 attatttgat tgatatcagc agacatttta tattgagttt cgattctacg acaattgatc
     8521 aattcttttg atcagttagc tttagcatgg atttccatca ggtgttgaac ggcggggacg
     8581 agttttttgg catccaccgg ttttctgagg aacccgtcga cgggcagcca aacggaagga
     8641 cctttttctg tacggtaatc gactccggct tttccggtga tcacatcgcg gacgaggagg
     8701 acatccagtt cgcggtagct gggatcctgg cggaattcgc gggccatttc ctgtactgcc
     8761 gatttagtag tagtcagtac atcgatagag gtcatcatta gtaccggcat attggcgaac
     8821 tcgggatttt cgctgaattc cctggccagt tcaaaacctt catactgggt agtcatcatc
     8881 acgtcgagga tggccaggtc cggtttttcc ttataagcca gtttaaaacc ttcgactttg
     8941 tcggaggcag agataacggt atagccttcc ttgctgagga tggctttaac aacagtgatg
     9001 atgtcaatgt cgtcgtcgac gatgaggatt tttttcgttt ccatggggtt gttttttaaa
     9061 gggttcattt gattacaatt tcatgataca aaaatagatt gcccggacaa cccgggtaag
     9121 gtggggaagt cctgattttc ggcgactgga ttccgatttt aatgagaaag aaagatggca
     9181 actgtgcgca agagttaccg gtagcaaaat gcaaataaac cggtaaaaat gtactggttt
     9241 tttgtatgat gaaatatcta tattattgat attaagtttt atatgcaatt tgagaagact
     9301 ggattttatt gcaaattttg acgtgaaaat tcagtacttt ctattgttaa ttatttcaca
     9361 atgggttata ttttaacccc tacagggtat gggtgaccgg ttgacgtcct gttaccacaa
     9421 tccatgccct
//
LOCUS       DAOPNH010000123         9407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_7979_length_9407_cov_4.812553, whole genome shotgun sequence.
ACCESSION   DAOPNH010000123 DAOPNH010000000
VERSION     DAOPNH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7979_length_9407_cov_4.812553"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1378)
                     /locus_tag="PKZ74_07825"
     CDS             complement(104..1378)
                     /locus_tag="PKZ74_07825"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPM92706.1"
                     /translation="MKKRYLYGLLSLIVLGLSILLPYLMPDQKSHVTTEAQPESLSVE
                     QHIEYGINCDSLRVEKGIVRQNQSLSEILSFYDIGSEIIHQIASLSRPVFDVRKMRVG
                     NPYTVLMTKDTVPEVVYFIYEEDAVSYVTYQLKDELKVNKGAKELTREISTASGVIES
                     SLWNSLAENGKDPTLANELSEVYAWTIDFFGIQKGDYYKVIYEELKVEGKPIGIGNVI
                     AACFHHMGSDLYAFRYTSNGETGYYDQKGENLQRAFLKAPLRFKRISSKFSHGRMHPI
                     LKIRRPHLGIDYAADYGTPVQTIGDGTVIEKGWNKGGGGNYIKIKHNGAYTTLYMHLA
                     GFAKGISTGTRVRQGDVIGYVGSTGLSTGPHLDFRVFLNGSAIDPLKMESPPSEPVAK
                     EYLPQYLPLVDSLKTGLDTLKVIRAQQLIVNR"
     gene            complement(1460..2044)
                     /locus_tag="PKZ74_07830"
     CDS             complement(1460..2044)
                     /locus_tag="PKZ74_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPM92707.1"
                     /translation="MKKDIILAGVGGQGILSIAAVIGMAALDSGLQLKQAEVHGMSQR
                     GGDVHSNLRISDREIFSDLIPFGKADLIISVEPMESLRYLPWLSKSGWLISNNVPHVN
                     IPGYPELASIESAIHAVGQHVLINADEIAKDLGMPRSANMVILGAASPWLGIDFSTLE
                     SAVRKIFGSKGADVVNQNLMALTAGKEFAMAHHS"
     gene            complement(2048..3673)
                     /locus_tag="PKZ74_07835"
     CDS             complement(2048..3673)
                     /locus_tag="PKZ74_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM92708.1"
                     /translation="MEKLLLLGDEAIAQSAIDAGLSGIYAYPGTPSTEILEYVHRSKE
                     AKENKIRASWSVNEKTAMEAALGMSYAGKRAMVCMKHVGLNVAADAFINSAITGANGG
                     LIVVAADDPSMHSSQNEQDSRFYGKFAMIPVLEPTNQQEAYDMVRAGFLLSEKYGIPV
                     LLRITTRLAHSRSGIIRREPLPQNEVHLPGNPKQFILLPANARRQYKSLLRNQVLFEE
                     ESESSDFNQYREGKDKSLGVITCGIAYNYLFENFQDREFPYPLLKIGQYPVPRKMIEK
                     LYSECNTILILEEGYPILEELLRGLLDDGKKIKGRMDGTVPRDGELNPNIVARAFGLD
                     EDYGFDVPGLVKSRPPSLCKGCPHIYSYNALNEALNLYSKGRVFSDIGCYTLGALEPY
                     GAINTCVDMGASITMAKGAADAGYVPAVAVIGDSTFTHSGMTGLLDAINDKSPVTVMI
                     LDNATTAMTGGQKSSATGRLEDICLGLGVEKEHIHILNPSPKNHEENLKIIEQEINYP
                     GVSVIIPRRECIQTVARRLRDEKKEKQKQEVIE"
     gene            3824..4417
                     /locus_tag="PKZ74_07840"
     CDS             3824..4417
                     /locus_tag="PKZ74_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPM92709.1"
                     /translation="MNFLAHLYLSGKEEDVIIGNFVADAIKGNSLGHYPQGMERGIRL
                     HREIDSYTDSHELFRASRNRLAPRYNHFSGIIVDLYYDHFLAKFWNEYSNEDLRGLVA
                     NTYFLLIRKFHMLPARSRRILPFMITQNWLVNYRDLDTMQRVFNGMSRRINKPSGMEN
                     AIGDLVAGYSLYEAEFRGFFPEITDHINRFRSNLIGL"
     gene            4444..5691
                     /locus_tag="PKZ74_07845"
     CDS             4444..5691
                     /locus_tag="PKZ74_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92710.1"
                     /translation="MASLTQEQKFEFIRNLPMFSGADNKVLLELADSMSENEALKGSI
                     VIRKGDEADAVFIIAGGRVRVHDGNHVLARLEQGQMFGEYSLIDQRQRSASVTAEDNC
                     RLLRLDNRDFYRIAAGDPGILRGVLKELINRMRDMNELEEKLSRSYLKIQRQKELIEK
                     QNASIMQQKAMLEQQNFDLTKLNEEKNQLIGTVIHQIKNPLTSSLCMADMLDARDGFV
                     DETEREGLAIIRNSIKRINNLINEILDINAIESKVYELKMEPLNVREILEDLIRNYSY
                     FIDQKHINFKVQMVDITARLNRVYVTQIFDNLLSNAIKFTPDGKTIEVRLGENEDKIM
                     FCVCDEGPGMEKEKMEKVFDRYKRQTSMFDQESAGNGLGLAIVYKYATAMHGTVRCES
                     EPGSGTRFHVELPGREKTKGNKE"
     gene            complement(5719..7032)
                     /locus_tag="PKZ74_07850"
     CDS             complement(5719..7032)
                     /locus_tag="PKZ74_07850"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HPM92711.1"
                     /translation="MIGLKEKLRQKIEGWRPRTKRLVQEFGDVVIDEVTIEKVIGGMR
                     DLKVLITDISYLDPNEGIRYRSYTLNEVIEKPPKPKGAEMPYVEGVWYLLLTGDIPTE
                     EEVQEVVEEFQKRRILPRVIYEVIDAAPCCNHPMTTFASAILALHRESFFVKKYNAGI
                     PKSEYWDPMYEDAMNLLAKLCEIATYIYTKLYKDGKRIQSNPNLDMGANFAWMMGINP
                     PYDDVSRMHFILHADHESGNVSAHTGHLVASSLSDVYLSIAAMINGLAGPLHGLANQE
                     VLRWLQDLREQLGGDDPTEEDIRKYVWDTLHNGHVIPGYGHAVLRKTDPRYTIQREFS
                     LKHMPEDPLFKYTDLLYKVVPEILIQQGKAKNPWPNVDAQSGIIQWHYGVRQYDFYTV
                     LFGIGRAIGICANIIWDRALMYPLERPKSITTEMLEEIVGIKQKE"
     gene            complement(7087..8457)
                     /gene="icd"
                     /locus_tag="PKZ74_07855"
     CDS             complement(7087..8457)
                     /gene="icd"
                     /locus_tag="PKZ74_07855"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPM92712.1"
                     /translation="MTGEKITKSGGRLNVPDIPIIPFIRGDGTGPDIWAASVRVFDAA
                     VEKAYHGKKRVEWLEVYAGEEAFNRFGEWLPQATIDTFRDYLVGIKGPLTTPVGGGIR
                     SLNVALRQLLDLYVCLRPVKYVTGVPSPVKHPERVDMVIFRENTEDIYAGIEWKSGSE
                     EVKKVIRFLEEEMKVKKIRFPETSGIGIKPVSEEGSKRLIRSAIRYALEENRHSVTLV
                     HKGNIMKFTEGAFKTWGYEVATQEFRDHVVTQRESWILGNIDKDPTISGEDNARLIEP
                     GYYMMTPEQQQAIKDEVAEGMKLLPTHGNGQWKTKLMIKDSIADITLQQVLTRADEFE
                     VVATPNLNGDYLSDALAAQVGGIGIAPGANINYDTGHAIFEATHGTAPKYAGLDQVNP
                     GSVILSGAMMFKYLGWHEVHDLIWKGLEKAVSGKRVTYDFHRQMEGAVLLKCSEFGEE
                     IVENMK"
     gene            8567..8980
                     /locus_tag="PKZ74_07860"
     CDS             8567..8980
                     /locus_tag="PKZ74_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HPM92713.1"
                     /translation="MKTTKKSGGSGFPTVFYDGSCHLCARSVRFILKYDRKKRFRFAS
                     LQSVTAKTLEIINLKDDSVILYEEGKILMKSQAAIRIAILLGFPWSVAGIFAVIPVSW
                     RDTIYDLIARNRHRWPGSSQYCETGRSTPDDRILP"
     gene            complement(8985..>9407)
                     /locus_tag="PKZ74_07865"
     CDS             complement(8985..>9407)
                     /locus_tag="PKZ74_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM92714.1"
                     /translation="ADISEKGFSWTNGVAVVPVVDLEDMTGTEREKWEKLTQKEREKM
                     IYSFEKPVPERVITQELRQTAFDNYQTTDDHGMHITGTAKDQNGQPYYYVKNSWGVTG
                     NDYKGFFFASKPFVAYKTIDILVHKDGIPKEIRKKLGL"
BASE COUNT         2311 a         2388 c         2230 g         2478 t
ORIGIN      
        1 caactactta gtgttccttc gtgatgtact ttgtgcgcct tagtggttaa taaaaagcct
       61 tttgagacag cctcagcaat aataacagag acgataaaag ggattaacgg ttcacgatga
      121 gttgttgcgc acggatcact ttcaaggtat cgagtccggt tttcagtgaa tccaccaatg
      181 gcaggtattg gggcaggtac tcttttgcaa cgggctctga agggggtgat tccattttca
      241 atgggtcgat ggcgctgccg ttgagaaaaa cgcggaaatc gagatgcggc ccggtgctga
      301 ggccggtgct gccgacatac ccgatcacat cgccctggcg tacccgggtt ccggtcgaga
      361 ttcctttggc aaatccggcc aggtgcatat agagtgtggt ataggcgcca ttgtgcttga
      421 tcttgatgta attacctccg cctcctttat tccatccttt ttctatcact gtcccgtcgc
      481 caatggtttg caccggggtg ccgtaatcgg cagcgtagtc aattcccagg tgcggacgcc
      541 tgatcttcag gataggatgc atccggccat gcgaaaactt ggaactgatt cgcttaaaac
      601 gaagaggggc tttcaaaaat gcacgctgaa ggttctcccc cttctggtca taataacctg
      661 tttctccatt acttgtgtaa cgaaaagcgt agaggtccga ccccatatgg tggaaacagg
      721 cggcaatgac atttccgatc cctatcggtt ttccttctac tttcagttct tcatagatca
      781 ccttatagta atctcctttt tgaataccaa agaaatcgat cgtccatgca taaacttccg
      841 atagttcatt ggccagggtc ggatcttttc cattttcggc cagggaattc cagagggaag
      901 attcgataac gccgctggcg gtgctgatct cgcgggtcag ctccttggcg cctttgttta
      961 ccttcagctc atcctttaat tgatacgtta cgtaggaaac cgcatcttct tcataaataa
     1021 aataaaccac ctccggaacg gtgtcttttg tcatcaaaac cgtataaggg tttccaaccc
     1081 tcatcttcct aacatcaaac acaggccgcg aaaggctggc gatctggtgt atgatctcgc
     1141 tgccgatatc ataaaaagag aggatctccg agagcgattg gttttgcctg acaatcccct
     1201 tttcaacccg cagggaatcg cagttgatac cgtattcaat atgttgttca acggaaagtg
     1261 attcaggctg tgcttcagtc gtaacatggc ttttctggtc cggcatgaga taaggaagga
     1321 ggatggaaag ccccagaacg attaatgaca gcaagccata gagatatctt ttcttcatga
     1381 atttattctt caaggaaggc caaaaataaa atttttcttg aaaggatggt ccgataattt
     1441 aacggccttt ccatgattct caggaatggt gtgccatagc gaactctttt ccagccgtta
     1501 aggccatcag gttctggttc accacatccg ctcctttgga gccgaatatt tttctgactg
     1561 ccgactcgag ggtagaaaaa tcaataccga gccacggaga agcagccccc aggatcacca
     1621 tattcgccga ccggggcatt ccgagatctt tcgctatttc atcggcattg atcagcacat
     1681 gctgaccgac ggcatgaata gccgattcga tactggcaag ctcaggataa ccggggatat
     1741 tgacatgagg tacattattg gaaatgagcc agccgctttt cgacagccag ggcaggtagc
     1801 gcagtgattc catcggttcg acggagatga tcaggtctgc tttcccgaat gggatcagat
     1861 cggagaaaat ctcacggtcg gagatccgga ggttggagtg gacatcccca ccccgttggc
     1921 tcatgccgtg cacttcggcc tgtttcaatt gaagcccgga atccagggct gccattccga
     1981 tcactgctgc gatcgacagg ataccctggc caccgacccc tgcaaggata atatctttct
     2041 tcatattcta ttcgatgact tcctgtttct gcttttcttt cttttcatcc cggagccgcc
     2101 gtgcaacggt ctgaatgcat tcgcgccgtg gaataatgac tgagacgccc gggtaattta
     2161 tctcctgttc tatgattttc aggttctctt catgattctt aggggatggg ttcaggatgt
     2221 gaatatgctc tttttccact cccaggccga ggcaaatatc ctccagcctg cctgtagccg
     2281 acgatttctg cccgccggtc atcgccgtgg tggcattatc gaggatcatg actgtgaccg
     2341 gggatttgtc gttgatagcg tcgagcaggc cggtcatgcc ggaatgggtg aatgtcgaat
     2401 cgccgatcac ggcaacagcg ggaacatagc cggcatcggc ggcccctttg gccatggtga
     2461 tcgaagcgcc catatccacg caggtattga tggcgccgta aggttccagc gccccgagcg
     2521 tatagcaacc gatatcgctg aatacccttc ctttggaata caaattcagc gcctcattca
     2581 gggcattgta ggaatagata tgcgggcatc ctttgcagag tgaaggtggc cgcgacttca
     2641 ccagcccggg tacatcgaag ccataatcct catccagccc gaatgcacgg gctacgatat
     2701 ttggattcag ttccccgtcg cgcggaaccg tcccgtccat ccttcctttg atctttttac
     2761 catcatccag aagacctctc aaaagctcct ccaggatcgg atatccttct tccagaataa
     2821 gaatggtatt acattcactg taaagcttct caatcatttt ccgggggaca gggtactgac
     2881 cgatcttgag caacggataa ggaaactccc tgtcctggaa attctcgaaa agataattgt
     2941 aagcaatccc gcaggtaatt acgcccaggc ttttatcttt tccttcgcgg tactgattga
     3001 aatccgaact ttccgactct tcctcgaaca aaacctggtt acgcagcaag gatttatatt
     3061 gcctcctggc gttggccggg agaaggataa attgtttggg gttgcccgga agatgaacct
     3121 cgttttgtgg caggggttcc cggcggataa tacctgaacg cgaatgggcc aggcgtgtgg
     3181 tgatcctgag aaggaccggg atcccgtatt tctccgataa caggaatccg gcccgcacca
     3241 tatcgtatgc ctcctgctgg ttggtgggtt ccagcaccgg gatcatggca aattttccat
     3301 aaaaacggct atcctgttca ttttgtgagg aatgcatgga aggatcatct gctgcaacaa
     3361 cgataaggcc accgtttgcc ccggtaatag ccgagttaat aaaggcatcc gcagccacat
     3421 tgagtccaac atgcttcata cataccatag ctcttttgcc tgcatatgac attcccagtg
     3481 ccgcttccat ggccgttttc tcatttaccg accagctggc ccgtatttta ttctcttttg
     3541 cttctttcga ccggtggaca tattccagga tctccgtgga aggtgttccc ggataggcat
     3601 aaattcctga taaacctgca tctatcgcag actgtgcaat cgcttcatcg cccagcagta
     3661 gcaatttctc cataataatt ctctacataa aagatattcg tagcctcact gacaaattta
     3721 gaataatttt cagaaagggg aatgccgtcg ggcttatcta caatcattat aaattagcag
     3781 ataaaagcat aaaatatttt caaaataatc acgttgattt tatatgaatt ttctcgctca
     3841 cctttatctt tcaggaaaag aagaagatgt catcatcggt aattttgtgg ccgatgcaat
     3901 caaaggcaat tccctcggtc attatcctca aggtatggaa agggggatca ggctgcatcg
     3961 cgaaatagac agctataccg acagccatga gcttttcagg gcaagcagaa acaggcttgc
     4021 accccgctac aatcattttt ccgggatcat tgtcgatttg tattacgatc atttcctggc
     4081 aaaattctgg aacgaatact cgaatgaaga cttgagaggc ctggttgcga acacctattt
     4141 tctgctgatc cgcaaatttc atatgttacc ggcaagatcc aggcggatac tgccgtttat
     4201 gatcacccag aactggctgg tcaattaccg ggacctggat accatgcaac gggtttttaa
     4261 cgggatgtcg cgacggataa acaagccgtc ggggatggaa aatgccatag gtgacctggt
     4321 ggccggctat tcgctctatg aagctgagtt cagaggtttt ttccccgaaa ttaccgatca
     4381 tataaatcgc ttcagaagta acctgatcgg tctatgatcg tctatcaata aatcaacaga
     4441 cggatggcat cacttaccca ggaacaaaaa ttcgaattca tccggaattt gcccatgttc
     4501 agcggagcag ataataaggt cctgctcgaa ctggccgatt ccatgtcaga aaatgaggca
     4561 ctgaaaggga gtatcgttat tcgcaaaggc gatgaagcgg acgcggtatt tatcatagcc
     4621 ggcggcaggg tcagggttca cgacggaaat catgttctgg ccaggctgga gcaggggcaa
     4681 atgttcggag agtattctct catcgatcag cggcagcggt cggcatcggt gacagccgaa
     4741 gacaattgcc ggcttctacg gctcgataac cgcgactttt atcgtattgc ggccggggat
     4801 cccggcattt taagaggtgt gctcaaggaa ctgatcaacc ggatgaggga tatgaacgag
     4861 ctggaagaga agctttcacg aagctacctc aagatacagc gtcagaaaga gcttatcgaa
     4921 aaacagaatg ccagcatcat gcaacaaaaa gcgatgcttg aacagcaaaa tttcgatctg
     4981 accaaattga atgaagaaaa aaaccagttg atcggcactg ttatccatca aataaaaaat
     5041 ccgctgacca gcagcctgtg tatggccgat atgctggacg ccagggacgg ttttgtggat
     5101 gaaaccgaac gtgaagggct ggccatcatc cggaacagca taaaaaggat caataatctt
     5161 atcaatgaga tccttgatat caatgccatc gaatcgaaag tttacgaatt gaagatggaa
     5221 ccattgaatg tcagggagat cctcgaagat ctgatccgga actattctta ttttattgac
     5281 cagaagcaca ttaactttaa ggtgcaaatg gtcgatatca cagcccggct gaaccgggtt
     5341 tacgtgacgc agatattcga caacctgctt tcgaatgcca tcaaatttac gcccgacggg
     5401 aaaacgattg aagtgagact cggggaaaat gaagataaga tcatgttttg tgtttgcgac
     5461 gaaggccccg gaatggaaaa agaaaagatg gagaaagtct ttgaccgcta taaacgccag
     5521 acatcgatgt ttgaccagga atcggccggc aacggactgg ggctggccat tgtttataaa
     5581 tacgcaacgg ccatgcacgg aactgtcaga tgcgaaagcg aacccggatc cggcacaagg
     5641 ttccatgttg aactgccggg aagggaaaag acaaaaggga acaaggaata aggaaaaggg
     5701 aaaaagtacc gggagaggct attccttttg ttttattccg acgatttctt ccagcatttc
     5761 cgttgttatc gatttggggc gctccagcgg gtacattaat gcacggtccc agatgatatt
     5821 ggcgcaaata ccaatagccc gcccgatgcc aaacaggacg gtatagaaat catactgccg
     5881 caccccgtaa tgccactgga taattcccga ctgcgcgtcg acgttcggcc atggattctt
     5941 tgcttttcct tgctgaataa ggatctcagg caccacttta tagagcaggt cggtatattt
     6001 gaacaacgga tcttccggca tgtgtttcag gctgaattca cgctgaatgg tgtacctcgg
     6061 gtcggtcttg cgcaaaacgg cgtggccgta gcccgggata acatgtccgt tgtgcagcgt
     6121 atcccaaaca tatttccgga tatcctcttc cgtcggatcg tcacctccga gttgctcccg
     6181 caggtcttgc agccagcgaa gcacttcctg gttggccagg ccgtgcagag gacctgccag
     6241 cccgttgatc atagcggcga tggacagata gacatccgac agcgaactgg ctaccaggtg
     6301 gccggtatgc gcactgacat tcccgctttc gtgatcggca tgcaggataa aatgcatccg
     6361 ggatacatca tcgtatggcg ggttgatacc catcatccag gcaaaattgg cgcccatgtc
     6421 gaggttgggg ttcgactgga tccgcttgcc gtctttatac agcttggtat agatgtaagt
     6481 ggctatttcg cagagcttgg ccagcaggtt catcgcatct tcgtacatcg ggtcccagta
     6541 ttcgcttttt ggtatgccgg cattgtattt ttttacgaag aaagattcac ggtgcagtgc
     6601 caggatagcc gaggcaaagg tcgtcatcgg gtggttgcag caaggggccg catcgatcac
     6661 ttcgtagatg accctgggca ggattctcct cttctggaac tcctccacga cctcctgtac
     6721 ttcttcctct gtcgggatat cacccgttag caggagatac catactcctt caacataggg
     6781 catttccgcg ccttttggct tgggaggctt ctcaatcact tcatttaacg tatagctcct
     6841 gtaacggatg ccttcattcg ggtccaggta ggatatatcc gttatcagca ccttcaggtc
     6901 cctcatcccg ccaatgactt tctcgatggt gacctcgtcg atcacaacat ctccgaattc
     6961 ctgcaccagg cgctttgtcc ttggcctcca gccttctatc ttttgccgta atttctcttt
     7021 cagtccgatc atttgcgata aagggttaaa tgttctattg ttctattgtc ctacagtctt
     7081 aaagtcctat ttcatattct ccacgatctc ttccccaaat tcggagcact tgagcagaac
     7141 agccccttcc atctggcggt gaaaatcgta ggtaactctt ttacccgaaa cggctttttc
     7201 caggcctttc cagatcaggt cgtgcacttc atgccagccg aggtatttaa acatcattgc
     7261 gccggagagg atcaccgaac cggggttcac ctggtcgaga ccggcatatt tgggcgctgt
     7321 gccgtgggtt gcctcaaaaa tggcatgtcc ggtatcgtag tttatattgg cgccgggtgc
     7381 aatcccgatg ccgccaacct gggccgccag ggcatccgac aggtaatcgc cgttcaggtt
     7441 cggcgtggcg accacctcga attcatcggc acgggtcagt acctgctgca gcgtaatatc
     7501 ggctatcgaa tcctttatca tcagcttcgt cttccactgc ccgttcccat gggtcggcag
     7561 cagcttcatc ccttccgcca cttcatcttt aatagcctgc tgctgttccg gtgtcatcat
     7621 ataataaccc ggttcgatca ggcgcgcatt atcttctcca gaaatcgtag gatccttgtc
     7681 gatatttccc agaatccagc tttcgcgctg tgtcaccaca tggtcgcgga actcctgcgt
     7741 tgccacttca tacccccacg tcttgaaagc gccttcagtg aatttcatga tattgccttt
     7801 atgcaccagc gtgaccgaat ggcgattctc ctcaagggca tagcggatgg ccgaccggat
     7861 aaggcgtttg gaaccctctt ccgaaaccgg ttttattccg atccctgatg tttccgggaa
     7921 gcggatcttc ttcactttca tctcttcttc caggaaacgg atcacttttt tcacctcttc
     7981 cgatccgctt ttccattcga tccccgcata aatatcttcc gtattctccc ggaaaatgac
     8041 catatcgacc cgctcgggat gttttacggg agaaggtacc ccggtgacat attttaccgg
     8101 gcgcaaacac acataaagat ccagtaattg cctgagtgcg acgttaagcg agcggatccc
     8161 ccctcctacc ggcgttgtca gtggaccctt tatccctacc aggtaatccc tgaaagtatc
     8221 gatcgttgcc tgtggcagcc actctccaaa ccggttaaaa gcttcttcac cggcataaac
     8281 ctcgagccat tccactctct tctttccatg atatgctttc tcaacagccg catcgaaaac
     8341 acgcaccgac gccgcccaga tatcagggcc ggtaccatcg ccacgaatga acggtattat
     8401 gggaatatcg ggtacattta atcttccgcc gctttttgta atcttctctc cagtcataat
     8461 acaggttctc ctaataatct gtgaatttgg ggataggtcg gtaaaaatac aaagttttta
     8521 atatcttgaa aaccatctat cttatttttt gttcattaca aataagatga aaacaacaaa
     8581 aaaatcagga ggttccggct ttccgaccgt attttatgac ggctcctgcc acctctgtgc
     8641 ccgttcggtg cgatttatcc tgaaatatga ccggaagaaa agattccggt ttgcatcgtt
     8701 gcaatcggtg acggctaaaa cattggaaat aattaatcta aaagatgact ccgtcatcct
     8761 ctatgaggaa ggcaaaatcc tcatgaaatc tcaggcagcc atcagaatag ccatactgct
     8821 cggatttccc tggtcggttg caggaatatt cgctgtgatc ccggtatcct ggcgcgatac
     8881 catttacgac ctcatcgccc ggaaccggca ccggtggccc ggaagttctc aatactgtga
     8941 aacaggccgt tccacaccgg atgaccgtat ccttccctga tttactacag ccccagcttt
     9001 tttcttattt ccttcgggat accatccttg tgaaccagga tatcgatggt tttgtaggcc
     9061 acaaaaggtt ttgaagcaaa gaaaaatcct ttgtaatcat tccctgtaac gccccaggag
     9121 ttcttgacat aataataggg ctggccgttc tgatctttcg ccgttccggt aatgtgcatc
     9181 ccatggtcat ccgtggtctg gtagttatcg aaggctgtct gtcgcagttc ctgggtaatg
     9241 acccgctccg ggaccggctt ctcaaagctg tagatcattt tttcacgctc tttctgggtc
     9301 agcttctccc atttttccct ttcggttccg gtcatatcct ccaggtcaac taccggaacg
     9361 actgctacgc catttgtcca ggagaaaccc ttttcgctga tatcggc
//
LOCUS       DAOPNH010000124         9386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8010_length_9386_cov_6.337263, whole genome shotgun sequence.
ACCESSION   DAOPNH010000124 DAOPNH010000000
VERSION     DAOPNH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8010_length_9386_cov_6.337263"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..118
                     /locus_tag="PKZ74_07870"
     CDS             2..118
                     /locus_tag="PKZ74_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPM92715.1"
                     /translation="MRLTRKNKEAGLMLDLQALDHIILTTEGYYSFAEEGCL"
     gene            complement(257..697)
                     /locus_tag="PKZ74_07875"
     CDS             complement(257..697)
                     /locus_tag="PKZ74_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92716.1"
                     /translation="MVKNKRKCLNLSVQASLIRRVFPTAGVTINRGKMVCKLLLQPSP
                     LSDSYNIKIVYDIDNHPDIYVIKRKLKLFSGATSLPHVYDTAKQHLCVYYRTAKEWNK
                     SMPIVDSIVPWTSEWLYHYEFWLATGTWHGKGIHGKPKPFRKTK"
     gene            complement(684..1913)
                     /locus_tag="PKZ74_07880"
     CDS             complement(684..1913)
                     /locus_tag="PKZ74_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPM92717.1"
                     /translation="MLNEEIKIQLNEIFEEISNNLDITESQFKKAVESYTAVGNWLSD
                     DESSLSPYKPMIKPQGSFLLGTMIRPLLETDELDVDLVCQLVGKNPTWTQFDLKNAVG
                     RRLKANQTYNQMLDKEGRRCWTLNYADSSKFHMDILPSLISNDYQIILEKAYSVRDYK
                     DYDKIAIRITDKLEENYYSETNPDFWNKSNPFGYAAWFQQKCLTNIEEARFLSDSMSP
                     MPNYQKRKYPLQQVVQILKRHRDIMFKGDEDKPISIIITTLAAKAYRQEQDLYVALFN
                     VVNSMEKYIEVKYSHEHNKWIKWIGNPVNEEENFADKWPDNPDKERNFFKWLQQIKMV
                     INNSSEIRGIPNIQLALKEALGQEAVNKAFTSIAENAIKTRESGELFMKGGTGYIGLK
                     DADNDIKVKQHNFHGEK"
     gene            complement(1916..3040)
                     /locus_tag="PKZ74_07885"
     CDS             complement(1916..3040)
                     /locus_tag="PKZ74_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HPM92718.1"
                     /translation="MSKSTLLPKTIIHLWTLAGGRCEYKGCNKSLWKDSLSMNKMNAA
                     YIAHIVSDSENGPRGDRIRSPKLAKDLNNLMLLCDEHHRKIDIEEKENHSESVLTEMK
                     KEHESRISFLTALKPNNKVHIVVYGANIGIQGSPLDYQDIVHAVLPNKYPANEYPIEL
                     GISNSSFQDNEEIFWNVEIKNLERQFQEKVEKIKNQDAVKCFALFGLAPQPLLIKIGT
                     LFSDLYDVDVFQKHREPSTWIWQDNFDFEGYTIEKPTELDGIPVLNLSLSANITDDRI
                     EKLFNSKTSIWKITHKNPNNDFLKSRDMLAKFRKTLRSFFDQVKFIHGQNAKLHVFPA
                     MPVSAAVELGRIWMPKADVKMIIYDQNKKKQGFYKTIEII"
     gene            complement(3051..3284)
                     /locus_tag="PKZ74_07890"
     CDS             complement(3051..3284)
                     /locus_tag="PKZ74_07890"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HPM92719.1"
                     /translation="MSRKTYHVTKTDSGWQGKVEGGKRASVTGETKEEVKQKTIDIAK
                     NQGDTSVIIHKGDGKIQEERTYPKSSDPYPPEG"
     gene            complement(4346..4422)
                     /locus_tag="PKZ74_07895"
     tRNA            complement(4346..4422)
                     /locus_tag="PKZ74_07895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4386..4388),aa:Asn,seq:gtt)
     gene            4693..5259
                     /locus_tag="PKZ74_07900"
     CDS             4693..5259
                     /locus_tag="PKZ74_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92720.1"
                     /translation="MKKNSPAFIFFLIISSVFIFSSCDKINDIIDPETETEKVTRIFV
                     EGSPWHVDTLTYKEDFLSGSQSVVTYDTSYFNYGTLEFTDPDASENPGYNCGYMIHRY
                     SENGQAKIDSMAWAPYNHNSGSDNHITIFIHDQNSIDFVVGAWDMYLDKIIIGENKVK
                     VEGWRRENAGGTSGGIYGNFRGYTLSRD"
     gene            complement(5283..6161)
                     /locus_tag="PKZ74_07905"
     CDS             complement(5283..6161)
                     /locus_tag="PKZ74_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPM92721.1"
                     /translation="MNIRKVELIMQPPEPHWVGDGFRVHNFVPGRYRLSMERMNPFIL
                     LDYHSKYHYPPTDKPKGVGVHPHRGFETVTIAYKGRVAHHDSSGGGGIIGEGDVQWMT
                     AASGVLHKEYHEKEWSRKGGDFHMVQLWVNLPAKDKMSEPKYQPITRESMGKVELPDR
                     AGTIEIIAGSYHGTPGPASTFSPVHLINARLNKGGKAGFEFPSSYNTALVVIEGSILI
                     NEEEKVPSDHFVLFENEGDEFTVKALENSIVLIMSGEPFHEPIAAHGPFVMNTPDEIK
                     QAYNDYYSGKFGELSD"
     gene            complement(6259..7566)
                     /gene="creD"
                     /locus_tag="PKZ74_07910"
     CDS             complement(6259..7566)
                     /gene="creD"
                     /locus_tag="PKZ74_07910"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPM92722.1"
                     /translation="METKNITQFMNSYSVKMIIVSVLALLLLIPSFLIQEIISERMSL
                     SEKVKKELYAHWGGKQVVAGPVLNVPFTATIPGNSDPVTSIRQGVAHFLPETLETIGT
                     LLPETRKRGIYRVVVYQGTLALKGTFAKPDLSQLDLENPVFNWGAAYFTIGVSDMRGI
                     RNLPVPVINGQSASVEPGVADTDLFSSGITVRAGSSDLNNPVTFEIELVLNGSENLSV
                     EALGKSSSVSLQSDWSQPGFTGEFLPSNRIVTDDGFTADWLVTHLNRNFPQQWTDRKY
                     STTEASFGVELLIPVDHYQKSMRSAKYAILFIALNFIIFLFIEIKSKTRIHPFQYSLV
                     AFALLLFYILLTSIGEQIGFNLAYLVSALAVTALISWYSYNLVRNKQTLAWVTLLQSG
                     LYLFLFAILQLQDYALLAGSIGLFLILAIIMKASQKIRWYAED"
     gene            complement(7620..7916)
                     /locus_tag="PKZ74_07915"
     CDS             complement(7620..7916)
                     /locus_tag="PKZ74_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPM92723.1"
                     /translation="MKNSIANLQKVFESRIRLGIMSALMVNETLTFNELKELLGVTDG
                     NLASHLKALEQQEIIGISKQFIGRKPNTTYKATMHGRYLFRQHLAALEKLIKNR"
     gene            complement(7994..8650)
                     /locus_tag="PKZ74_07920"
     CDS             complement(7994..8650)
                     /locus_tag="PKZ74_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92724.1"
                     /translation="MKNNSTNPGDDLQAIRDIMERSSRFLSLSGLSGIFAGACALAGA
                     AIAWLFILDSGNIRYDEYWHSAGVTHSFSMRNYLILDALLVLAAALLGAAYFSRRKAM
                     KAGTNLWTVSTKRLILHLTIPLFTGGIFILILAYRGNIELVVPAMLIFYGLALVNAGK
                     FTFGEIHYLGLTQIGLGILAAIFTGYGLIFWTAGFGLMHIIYGAVMYLRYERVEDIET
                     "
     gene            complement(8827..>9386)
                     /locus_tag="PKZ74_07925"
     CDS             complement(8827..>9386)
                     /locus_tag="PKZ74_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92725.1"
                     /translation="FAVGDRLQLLVRVKSVANGETYMKAHPGTVISMPSSANGVFLAG
                     GPWEDYSTPNRDLRLLIAMDAVLGFPDRVAAFPQDFKISGRKTAEETRQSLRSMLEKK
                     VAGQTVSYTRSDGSMQELTVAEILLRKDAFEMAYNPNDCPEIRWGAPENSPERSTCRR
                     TAPPYQRKTMESVRKWFQQRLHPPT"
BASE COUNT         2444 a         2046 c         2042 g         2854 t
ORIGIN      
        1 cattcgccta acgaggaaaa ataaagaagc aggtttgatg ctcgaccttc aagcccttga
       61 tcacattatt ctaactactg agggttacta ttcctttgct gaagagggat gcctctaaca
      121 ataaccgcaa tactgatctt caaaaagagg gtgcttccgg tcagtctggt tcagctgata
      181 tgaaggtgct gggaaagatg gagagggtag ggtgaggagt ttggtcagat tattaatcct
      241 tctaaaataa tgctggtcat tttgttttcc tgaatggttt tggctttcca tgaataccct
      301 taccatgcca agtgccagtt gctaaccaaa attcataatg atacaaccat tcagatgtcc
      361 atggaactat tgaatccaca atcggcatac ttttattcca ttcttttgca gttctgtaat
      421 atacacataa gtgttgtttt gctgtatcat aaacatgagg cagacttgtt gcaccagaaa
      481 atagtttcaa ttttctttta ataacatata tatcgggatg attgtcaata tcataaacaa
      541 tctttatatt atatgaatca cttaaaggag atggctgaag caaaagctta cagaccattt
      601 ttccccgatt tattgtaact cctgctgttg gaaatactcg tctaataagt gatgcttgaa
      661 cactcagatt gaggcattta cgtttatttt tcaccatgga agttatgttg tttaaccttt
      721 atgtcattgt cagcatcctt taagccaata tatcctgtgc ctcccttcat aaatagctcg
      781 cctgactctc ttgtctttat ggcattctct gcaatggaag tgaatgcttt atttacggct
      841 tcctgcccta atgcttcttt caatgccagt tgtatattag gtattcctct tatttctgat
      901 gaattattta taaccatctt tatttgttga agccatttaa agaaatttct ttctttatca
      961 ggattatcag gccatttatc agcaaaattc tcctcttcat tgacaggatt cccgatccac
     1021 tttatccatt tattatgctc atgagaatat ttcacttcaa tatatttttc cattgagtta
     1081 acaacattaa aaagagcgac atacaagtcc tgttcttgcc gatatgcctt tgctgctaaa
     1141 gtggttatta tgattgatat aggcttatct tcatctcctt taaacataat atccctatgc
     1201 cgctttaata tttgtacaac ctgttggagt ggatattttc tcttttgata atttggcatt
     1261 ggactcatag aatcacttaa gaatctggct tcttcgatgt tagtaaggca cttttgttga
     1321 aaccatgcag cataaccaaa aggattactt ttattccaga agtctggatt tgtttccgaa
     1381 tagtaatttt cttccagctt atctgtaatc cttattgcga ttttgtcata atctttataa
     1441 tcccttaccg aataagcttt ctctaagatt atttgatagt catttgatat cagggatggt
     1501 aaaatatcca tatgaaattt tgaagaatca gcataattta aagtccagca tctccgacct
     1561 tctttatcta acatctggtt ataggtttga ttagctttaa gtcttcttcc gacagcattc
     1621 ttcaaatcaa attgtgtcca tgttggattt tttcctacaa gttggcatac taaatctaca
     1681 tctaattcat ctgtctctaa taagggccta atcattgttc caagtagaaa agaaccttgt
     1741 ggtttaatca ttggtttgta aggtgataat gaagattcat cgtcagataa ccaatttcca
     1801 acggccgtat aactctctac cgcttttttg aattgagatt cagttatatc cagattattg
     1861 gatatttctt caaatatctc atttaattga attttaattt cttcgtttag cataattaaa
     1921 taatttcaat tgttttataa aaaccctgtt tcttcttgtt ttgatcgtaa atgatcattt
     1981 taacatcagc tttaggcatc caaattctgc ctaattctac cgctgctgat acaggcattg
     2041 ccggaaagac atgtaatttc gcattttgtc catgtatgaa cttaacctga tcgaaaaagc
     2101 ttcttaaagt ttttctaaac ttagcaagca tatctcttga ttttaaaaaa tcattgttcg
     2161 gatttttatg agtgatcttc caaatgcttg ttttcgaatt aaatagcttt tctattctat
     2221 catctgtaat atttgcactt aacgataaat ttagcaccgg aatgccatct aattcagtag
     2281 gtttttcaat ggtatatcct tcaaaatcaa aattatcctg ccatatccac gttgaaggtt
     2341 cacgatgttt ttgaaaaaca tcgacatcat ataaatcgga aaacagtgtt cctattttta
     2401 ttagtaaggg ttgaggagct aatccaaata atgcaaagca tttaacagca tcttgatttt
     2461 ttattttttc gaccttttct tgaaattgtc tttcaagatt ttttatctcc acattccaaa
     2521 atatttcttc attatcctgg aagctactgt tagatatacc taactcaatt ggatattcat
     2581 tagcagggta cttattaggc aaaactgcat gtacaatatc ctgataatcg aggggtgagc
     2641 cttgaattcc gatgtttgct ccgtatacga caatatggac tttattattt ggtttgagag
     2701 ctgtcagaaa tgaaattcga gactcatgtt ctttcttcat ttcggttaat accgattcgg
     2761 aatgattttc tttttcttca atatctatct tcctatgatg ttcatcgcac aatagcatca
     2821 gattgtttaa atcctttgcc agtttaggcg atcttatcct atcacctcgg ggtccatttt
     2881 cactatcaga aacaatatga gcaatataag ccgcattcat tttattcatg gataggctat
     2941 ccttccacag agacttgtta caacccttat attcacatct tcccccagca agagtccaaa
     3001 gatgaattat tgttttcgga agtaaagtag atttagacat atgctttatt ttagccttct
     3061 ggcggatatg gatcactgct tttcggatag gttcgttcct cctggatctt accatcaccc
     3121 ttatgaataa taactgatgt atcgccttga tttttggcga tatcaattgt tttctgctta
     3181 acttcttctt ttgtttctcc tgtgactgaa gctctttttc ctccttcaac tttaccttgc
     3241 caacctgaat cggttttggt aacatggtat gttttcctac tcatatctaa aaaattaagt
     3301 ttatgattat taatatttta tatcatgtat gccaaactct tcaaaaattg acccatccac
     3361 aaatttttga attaaaactt atcaatattc tcatgttgac aacttggata tgttataaga
     3421 cactgttgat caatcacata attcagtaaa tactttttaa caccctatat gaaggaaata
     3481 ttgcagatta gttgatgtga ctttatgtca gtagattatg acaaaacaat gtactaagtg
     3541 agggtataag tccttactga cagaatttca ttataaattt tgaaaatgag cttttaacta
     3601 aagtttcatc gtggtagaaa cccgctcccc catttccaca tctgcccata tcatcatacc
     3661 attttccgat ttgaatgcct caaagactcg ctactggctt cataacttaa gggattaatc
     3721 aaggggagca accaggaagt tttagatcaa agaaattgaa tctaaaatta atcaatggaa
     3781 tccttcagcc agttaagtga aaaatcaaaa aatattgatg tcttcaaacc aaactttaaa
     3841 tttatgaact acctttactt tctaaactat ttgatataag ctgtatatga agatatattc
     3901 acctcagata atgtaataat acattgcctg aggtatccaa gtggctgaag gaacccgttt
     3961 gataatcggg tatactcgaa agagtatcgc gggttcgaat cccgtcctct ccgctaaata
     4021 tgggggctta ggtccccttt cggattttta gagtatagtg gctccgccat caaaacccac
     4081 tggtttaaaa agggtatcat ccagcttatt tcaaaaccaa gtacctaaat ccaaccccga
     4141 cccggactcg aactacagcc agaacacttt ccatcagcca gttaactgat taaccagaag
     4201 aaaatccgga tattttgcct ttttcagaaa ataatgtatc taaaaatttt ttgtaggata
     4261 ctggaatgag ctaatccttg tctatcatag aatacgaaag ggttaaaaat gaaaaagtcc
     4321 cgtaaaaacg ggacttagtc gactttggct ccccgacctg gactcgaacc agggacctaa
     4381 tgattaacag tcattcgctc taaccagctg agctatcggg gaagatagta ttcaggtttt
     4441 ttgagggcgc aaaggtaata attaattttg aatgttgagt gtagaatgtt gaatttggga
     4501 agattttttt tgggggatgg tatttcatgt tgatccgggc agaatcttcc gggttgaaga
     4561 tccggctaca ctcaggcagg cctggcctca aactttaaca tcttcttaac aatattccgg
     4621 atatgtttac ttgactcgta tgccggcgta tcgatacttt tgcggccgtt ttaaaaatat
     4681 actttgttaa ctatgaaaaa gaactcaccg gcttttattt tttttctgat catcagctct
     4741 gtctttatct tcagttcttg cgacaagatc aacgacatta tcgatcccga aacggaaacg
     4801 gagaaagtta cccggatctt cgtagagggc agcccatggc atgttgatac attaacctat
     4861 aaggaagact ttttaagcgg cagtcagagc gtcgttacat acgatacctc ctatttcaat
     4921 tacgggaccc tggaattcac cgatcctgat gcttcagaaa atccgggtta caactgcggc
     4981 tacatgatcc accgctattc ggagaacggg caggccaaaa tagattctat ggcatgggcc
     5041 ccgtataacc ataactccgg atcggataat cacatcacca tcttcatcca tgatcagaat
     5101 tccattgatt ttgtcgtcgg ggcatgggat atgtacctcg ataaaataat catcggcgaa
     5161 aataaggtta aggtggaagg ctggcgaagg gaaaatgccg gaggtacctc cggcgggatc
     5221 tatggaaatt tcaggggtta taccttaagc cgtgattaat tcggatcagc tttcggattg
     5281 tgctaatccg aaagctctcc gaacttcccg ctgtaatagt cgttgtatgc ctgcttgatc
     5341 tcatccgggg tgttcatcac aaacgggccg tgggccgcaa tgggctcgtg aaacggctcg
     5401 ccgctcatga tcagcacaat gctgttttcc agcgctttaa cggtgaattc atccccttca
     5461 ttctcaaaca gcacgaaatg atccgaagga actttttcct cttcattgat aaggatgctt
     5521 ccttctatca ccactaaagc ggtgttgtat gaagaaggaa actcaaaacc ggctttaccg
     5581 cccttgttca gcctggcgtt gatcaggtgg acaggggaga aggtgctggc cggtccgggg
     5641 gttccgtggt atgatccggc tatgatctcg atagtgccgg cacggtcagg caactccacc
     5701 ttgcccatgc tctcacgggt gatgggctga tatttcggct ccgacatctt atctttcgct
     5761 ggcaggttca cccagagctg gaccatatgg aaatcgcctc ctttccggct ccactctttt
     5821 tcgtggtact ctttgtgcag caccccggat gcggccgtca tccactgcac atcgccttca
     5881 ccgatgatgc cgcctccgcc gctgctgtcg tggtgcgcca cgcggccttt gtaggcgatc
     5941 gtcaccgttt caaagcccct gtggggatgg acgccaaccc ctttcggctt atcggtcgga
     6001 gggtagtggt atttcgagtg atagtcaagc aggatgaaag gattcatccg ctccatgctg
     6061 agacggtaac ggccgggcac aaaattgtgc acccggaacc cgtcgcccac ccagtgtggt
     6121 tcgggcggct gcatgatcag ttcaaccttt ctgatgttca tttcttaagt actttaatat
     6181 caaattatcg tatgagaatc ttttctgtaa cttaaacaat caacatgttg aaaggtttaa
     6241 tccgggatag ctttcatact aatcttccgc ataccaccgg atcttttgag aggccttcat
     6301 gatgatcgcc aggatcagga acagtccgat acttccggcc agcagggcat aatcctgaag
     6361 ctggagaatc gcgaacagga acaggtagag gcccgattgc agcagggtaa cccaggccag
     6421 ggtctgttta ttacggacca ggttgtagct gtaccaggag atcagcgccg taacggccaa
     6481 agccgataca aggtaggcca ggttgaaccc gatctgttcg ccgatggagg tcagcaggat
     6541 gtaaaacagc agcaaggcaa atgcgaccag ggagtactga aatggatgga tgcgggtctt
     6601 gcttttgatt tctataaaga ggaagatgat gaaattgagc gctatgaaca ggatggcata
     6661 tttggccgag cgcatcgatt tctggtaatg atcgaccggg atgagaagtt caacgccaaa
     6721 cgaagcttca gtcgtgctgt atttccggtc tgtccattgc tgggggaaat tccggttgag
     6781 gtgggtcacg agccagtcgg ccgtgaatcc gtcgtcggta actatacggt ttgacggaag
     6841 aaattcaccg gtgaagccgg gctgcgacca gtcggattgg agagaaacgg aggaagattt
     6901 acccagggct tccaccgaaa ggttctccga gccgtttaaa accagctcga tttcaaatgt
     6961 taccggatta ttcaggtctg aagacccagc cctgacggtg atccccgagc taaaaaggtc
     7021 ggtgtcggcc acgcccggct cgacggaggc gctttggccg ttgatcaccg gcaccgggag
     7081 gttcctgatg ccccgcatat ccgagacgcc gatggtgaag taggcagctc cccagttaaa
     7141 taccggattt tcgaggtcca gctgtgaaag gtcgggtttg gcaaaggtcc ctttcagcgc
     7201 cagggttccc tggtagacca ctaccctgta gattccgcgt ttccgtgttt ctggtaataa
     7261 ggtgccgatt gtttccagcg tttcgggtaa aaagtgcgcc actccctgcc tgatgctggt
     7321 caccgggtcg gaatttcccg ggatggttgc cgtaaagggc acattcagaa ccggtccggc
     7381 tacaacctgt tttccgcccc agtgtgcata gagttctttt tttacttttt cactgaggga
     7441 cattcgttcg ctgatgattt cctgtatcag gaaggagggg atcagcagca gcaaagccag
     7501 gaccgacacg atgatcattt tgacgctgta actgttcatg aactgtgtga tgttttttgt
     7561 ttccatattt atagaattaa gaaagtactt tgtattacaa agtgaatgat taaaaaaaat
     7621 tatctgtttt taatgagttt ttcgagagca gccaggtgct ggcggaagag gtaccggccg
     7681 tgcatcgtgg ctttataggt ggtgttcggt ttgcggccga tgaattgttt ggagatgccg
     7741 atgatttcct gttgttccag ggctttcagg tggctggcca ggttcccgtc ggtcacgccg
     7801 agcagttcct tcagctcgtt gaacgtcagg gtttcgttga ccatgagggc cgacatgatc
     7861 ccgagcctga tgcggctttc aaatactttc tgaaggttcg caattgaatt tttcactggt
     7921 tttatttctt atttgttccg ggttccgggt tccaggttcc aggttccagg ttccaatttt
     7981 ctaatttcta atttcaagtt tctatatcct ccacccgctc atacctgaga tacatcactg
     8041 cgccgtaaat gatgtgcatc aggccaaaac cggctgtcca gaatatcagc ccgtaaccgg
     8101 tgaagatggc ggctaggatg ccgagcccga tctgtgtaag tcccaggtaa tggatctcgc
     8161 caaaggtgaa cttcccggca ttgaccagcg ccagcccgta gaagatcagc atggccggca
     8221 ccaccagttc gatgttgcca cggtaagcca ggatcagtat gaagattccg ccggtgaaaa
     8281 gcgggatcgt cagatggagg atcagccttt tagtggaaac ggtccacagg ttggttcctg
     8341 ctttcatggc ctttctccgg gaaaagtaag ccgcacccag cagcgccgct gccaggacca
     8401 gcagagcatc aagaataagg taattccgca ttgagaatga atgtgttaca cctgcgctgt
     8461 gccagtattc atcgtaacgg atgtttccgg agtccaggat gaacaaccag gcaatcgccg
     8521 ccccggccag tgcgcaggca ccggcaaaga tgccggataa cccgctcagc gataagaacc
     8581 gtgacgaccg ttccatgatg tcccggatcg cctggagatc gtcgccggga tttgttgaat
     8641 tgtttttcat ttagaaagta ctttgagatg caaagttata ataaaaagga aaacgatggt
     8701 cgattttcaa tttgagatct tttttttttt gagattcgag atttggaatt tgtttggatt
     8761 ttggtgcctg gaatttggaa tttgtttggt cttgggtact cgggatggtg cctggaactt
     8821 ggaattttac gtcggcgggt gcagacgctg ctgaaaccac ttcctgaccg attccatggt
     8881 tttgcgctgg tagggagggg cggtacggcg gcaggtcgac ctttcggggc tgttctccgg
     8941 agctccccag cggatctcgg ggcaatcgtt cgggttgtaa gccatctcaa acgcgtcttt
     9001 gcgaagcagg atctcggcca cggtgagctc ctgcatcgag ccatccgagc gggtatagct
     9061 aacggtctgc ccggccactt ttttctccag catcgaccgg aggctctgcc gggtctcttc
     9121 ggctgttttc cgcccggaga tcttgaagtc ctgcgggaaa gcggccaccc tgtcagggaa
     9181 tcccagcacg gcatccatag cgatcagcaa ccggagatcg cggttcgggg tggagtagtc
     9241 ttcccagggg ccgcccgcca ggaagacgcc gttagcgctt gaaggcatgg agataactgt
     9301 tccggggtgc gctttcatat acgtttcgcc gttggcgacc gatttcaccc gcaccagcag
     9361 ttgcaatcgg tcgccaacgg cgaaac
//
LOCUS       DAOPNH010000125         9339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8064_length_9339_cov_4.887010, whole genome shotgun sequence.
ACCESSION   DAOPNH010000125 DAOPNH010000000
VERSION     DAOPNH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_9339_cov_4.887010"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PKZ74_07930"
     CDS             complement(<1..957)
                     /locus_tag="PKZ74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM92726.1"
                     /translation="MINTQFPEKPALILRDHTDTYQQLIQRIREYAALFAGKNIEKVA
                     IFSENRPEWIYAFYSAWYNSALVVPIDFLSSAADAAYILNDCRPELLFAGKSVSATLA
                     KMYGKLEFKPEIIYFEDVRLTGDTIYSEWDFHPIEDKTAAIIYTSGTTGSPKGVMLSF
                     KNISANIKAVTEDYPIFTSDRPVLMLLPLHHIFPLAGSMVAPLKAGGCVVMSPSMQSS
                     DIIETLKAHPVRIMIGVPRLYELLYKGIKAKIDAKAIGRLFYRIVKLSRSKRLAKKIF
                     KKVHAGLGGELEIMVSGGAALNKAVGQFFDTLGFAVMEGYGMT"
     gene            1217..3040
                     /locus_tag="PKZ74_07935"
     CDS             1217..3040
                     /locus_tag="PKZ74_07935"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPM92727.1"
                     /translation="MSTNKEAWGSRIGLILAMSGNAVGLGNFLRFPVQAVQNGGGAFI
                     IPYLVCFLLMGIPLLFVEWSIGRYGGRFGHHTTPFTLEKMGGNKVWRYVGVFGIFSNI
                     AIAAYYCYIESWTLSYTMHSALGSFKGLDQHGVADFFSNYLDLSTTTTGIPYEAVVFF
                     LVCMFLNTWVLSRGIAGGVEKMAKIAMPLLVFFAIFLAIKAITLKAGHENAVNDGWAG
                     LSFLWTPQFDSLTNPKVWLAAAGQIFFTLSLGQGSVQCFASYVKKKDDIALNAMSAGW
                     MNEWVEVVLGASIIIPISIGYLGIDRVIELTNLGGLGLGFRTMPYLFQQWGPILAAMA
                     GIAFFGLLFFAGITSSLAMGTPILSFMQDEFNWSRKNSALVFGGAVTALGLPTVFFFQ
                     KGVLDEYDYWAGTVSLVVFAMLEIILFSWIFGIKKGWKEITAGADIRIPGIYKFIIKW
                     ITPFILIIVFLGSMVRPVNDDWTKVSLRHWPLHKESIIGQLIHKDIGPNKAYFTDAFY
                     AEFEGTVDSISSMNQKTILQVSSVDQTRSETYILGKKTEPAVKTGDQFQAGDTLYTGK
                     VVNRILYTDLARLLLLSLFLFSSVLVYLAAVKRKKYADRHH"
     gene            3052..3204
                     /locus_tag="PKZ74_07940"
     CDS             3052..3204
                     /locus_tag="PKZ74_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92728.1"
                     /translation="MNTSAIIFMALAQTIITLITFSFLYRAMRAKKRPEPDSYADNDD
                     DDDDEN"
     gene            3323..4279
                     /locus_tag="PKZ74_07945"
     CDS             3323..4279
                     /locus_tag="PKZ74_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPM92729.1"
                     /translation="MINRIKDYFSFTAKEQRGLIALLGIILLALSAGIYLPVIIPQKQ
                     FDIEPFRSEVEAFLAKAVIEDSSAISNQNQALKFTPVSDHQDLEGFQQSPYPFNPNEL
                     DESQWKNMGLRDRIVNNIMNYRKKGGRFRDKEGFRKIYGLTDKEFGILESYLVFDPPV
                     KSEYPKDTASKPDGFHDQYTKKDFLPIRVEINSADSAALLNLKGIGPSFAGRIVKYRS
                     RLGGFYKPEQLMEIRGLDSVRFNQFRDQIELNSGLISKIDINNVTFKELLKHPYFEYY
                     LVKAIFEKKDQIKSFDSVEQIKYLPVMYEELFEKISPYITVN"
     gene            complement(4272..5054)
                     /locus_tag="PKZ74_07950"
     CDS             complement(4272..5054)
                     /locus_tag="PKZ74_07950"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM92730.1"
                     /translation="MIRQEERKILRSMVIPFMLVLLMWAVKIIEVTGDYKLSFLGIFP
                     LRWQNLTGIITTPFIHGDFSHLIANSVPLIILGSALFYFYKDLSFRILLFIWLFSGFW
                     VWIAARPAFHIGASGIVYGLATFIALSGIIRKHTGLMALALIVVFLYGSLIWGIFPEF
                     FPEKNISWEAHLYGMIAGIILAVYYRKQGPQRKVYEWELEEEEETGRHGDQETRRHGD
                     QETGRHGDQETGRQGDDFWNVSITDEEIDKIRHIYRPNKNVN"
     gene            complement(5127..6164)
                     /locus_tag="PKZ74_07955"
     CDS             complement(5127..6164)
                     /locus_tag="PKZ74_07955"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPM92731.1"
                     /translation="MKSLFCNWVCLILMGLTTGIQGQSARIVFYNVENLFDVMDDTLT
                     NDEEFLPEGDKNWTYDRFREKTIKIYRTLVALGQGDMPAVIGLCEVENRLVLNRLIYD
                     TPLSLKDYRLIHRESHDARGVDVALIYRADLFFPITSEWLTIPLRSGETTREILHVKG
                     RIWDRDTLDIYINHWPSRYGGAGTSVQKRMASAAILSESIVKVLKSNYNANIIVMGDF
                     NDEPDDESMQSVNKILENSLQDSFCPLVNLSSLEKNIFGRGTIKHQGVWSVFDQILVS
                     CALFSGDSGLRVSDQMAGIFMAEFLLEPDNAYSGMKPFRTYLGPGYHGGFSDHMPVFL
                     DVEKTGTLKRK"
     gene            6293..8671
                     /locus_tag="PKZ74_07960"
     CDS             6293..8671
                     /locus_tag="PKZ74_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92732.1"
                     /translation="MTKAHFSQISSLLTGIIFLTCLFFNATAGIDPDDPSVKRYTISG
                     HIKDNTSGEDLFGSSVLVTEQNTGTIANEYGFYSVSLTKGIYTLRFSYMGYETQTHKI
                     TADKDIVLDINLNPSSAILNEVEIRAERGDANIKTPEMSVVKLDVKTINRIPALMGEV
                     DIIKAIQLLPGVQSVSEGSSGFSVRGGSPDQNLVLLDEATVYNPSHFLGFFSVFNNDA
                     IKDVKLYKGDIPAQYGGRLASVLDVRMKDGNQKKFSGTGGIGLISSRLTLEGPIIKDK
                     TSFILSGRRTYADLFLPLSKNEGVQDSRLYFYDFNAKINHTFNENNRLYLSGYFGNDI
                     FKNPFAYMKLGNTTATIRWNHLFSKRLFSNFTAIYSRYQYGLGTADDDNPNSFLWTSV
                     LQDYQVKGDFTYYPGTNHTLRFGFTSSLHLLDPGSASGRGDSSLFTEYSIDRNHSLES
                     GLYVSNDQLIGSRLTLKYGIRFSLFQNIGPSTVFLYDDEFHLSDSTKYEHGKFYNTYT
                     GLEPRLGLTYVLDEFSSVKASYTHTYQYLQLAQNSTAGTPLDVWYSASPNVKPQISDQ
                     AAIGYFRNFRKNTIETSVEVYYKTMRNTIDFKDHAELLLNKQLEGELRFGKGWSYGVE
                     LMVSKPQGKITGWVSYTFSRTWRKFEDINDGKKYPAPYDSPHDVSIVLNYDATKRISI
                     SANWIYSTGKPVTFPTGRAVIDGVVVPIYSDRNAYRMMDYHRLDLSVALHKKPSDSRF
                     DWDLVFSVYNAYNRHNTWAINFVQDNLYPLTTYAEQTYLFGIIPAVTFNFQF"
     gene            8671..>9339
                     /locus_tag="PKZ74_07965"
     CDS             8671..>9339
                     /locus_tag="PKZ74_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPM92733.1"
                     /translation="MMKRIHILYLLIPVILSSCTERIDIDLDETYTRLVVYGVLTTDT
                     TTHYVTLSKTTSYFYNELPPPVSGAEIIISDDQGNEFTLQETEPGKYATSPLFAAVQG
                     RTYTLNISLEEEINNFRNYTASTKVTSINPIDSIGLVHQPLWGEKGFYEVTCYYQEPP
                     TRDFYMFNILINGKLVTDTINSRIVINDDFFNGNYTNGIGVGYLDQSKTDEILRPGDK
                     VTFQG"
BASE COUNT         2618 a         2150 c         1997 g         2574 t
ORIGIN      
        1 ggtcattcca tagccctcca ttacagcaaa acccagtgta tcaaagaatt gaccaactgc
       61 cttattcaac gcagcgcctc ccgaaaccat gatctccagc tcgcctccga ggcccgcgtg
      121 aactttttta aaaattttct ttgctaacct cttgctcctg gagagtttga ctattctata
      181 aaagagcctg ccaattgctt tggcatcgat tttagcttta atccccttgt aaagaagctc
      241 atacagcctg ggtactccta tcattatccg cacaggatgg gctttcaggg tttcgataat
      301 atcggatgac tgcatggatg gagacatcac tacacatcct cctgctttga gcggagcaac
      361 catcgatccg gccaaaggga aaatatgatg aagaggaagc agcatcaata cggggcggtc
      421 gctggtgaag atcgggtaat cctctgttac cgccttaata tttgccgaga tgtttttaaa
      481 agataacatg acccctttgg gacttccggt tgtgcctgaa gtataaataa ttgccgccgt
      541 tttatcctct atgggatgaa aatcccactc cgaataaata gtgtctccgg ttagccgaac
      601 atcttcaaaa taaataattt ccggtttaaa ctccagtttg ccatacatct ttgccaatgt
      661 agcggataca cttttaccgg caaacaacag ttccgggcgg cagtcgttca gaatatatgc
      721 tgcatctgcc gctgaagaca gaaaatcgat gggtacaacc aaggcactat tgtaccaggc
      781 agaataaaac gcatatatcc actcggggcg gttttctgaa aaaatggcaa ctttttcaat
      841 gttctttccg gcaaagagtg cggcatattc tctgatacgc tgaatcaact gttgataagt
      901 atccgtatga tcccggagga tcagagccgg cttttcaggg aattgagtgt ttatcatctg
      961 ggggttgggt aagtgaaaga ttaatacaaa tttagtatta atttcatatc ccggaatcta
     1021 aatatcagga tacaaatccc gggtaatatt tcctttcaaa tgtcagtgta taactgtatt
     1081 acacgtttaa gtcaatattt ttgattataa atacatgtaa tttattaagg atcgggattt
     1141 ggtattttgg caaattccca tacctttaat cgcgattttc tttgaatcct ttaattaatt
     1201 aactaaacca ggacgaatga gcactaacaa agaagcctgg ggatccagga tcggcctcat
     1261 tctggccatg tcgggaaatg cagtaggatt gggtaacttc cttcgctttc cggttcaagc
     1321 cgtacaaaat ggaggtgggg cattcatcat cccttatctg gtgtgtttcc tgctgatggg
     1381 aatcccgtta ctttttgtgg aatggtcgat cggtaggtat ggaggccggt ttggacatca
     1441 taccacccca ttcactcttg aaaaaatggg tgggaataaa gtctggcggt acgtgggggt
     1501 gtttgggatt ttttcaaaca tagccattgc tgcttattat tgttacatcg aaagctggac
     1561 actttcctat acaatgcatt cggctttggg atcttttaag gggcttgacc agcatggtgt
     1621 ggctgatttc ttctccaatt accttgacct ttcgactact accaccggaa taccttacga
     1681 agccgtcgtt tttttcctgg tctgtatgtt tctcaatacc tgggtcctga gccggggaat
     1741 agctggcggt gtagaaaaga tggcaaaaat tgctatgcct ttgcttgtct ttttcgctat
     1801 atttttggca atcaaggcca ttacgttaaa ggccggccat gaaaatgcag tcaatgacgg
     1861 ctgggcagga ctcagtttcc tgtggacgcc ccaatttgac tcgcttacca atccaaaggt
     1921 ctggctggcc gccgccggac agatattttt cacactctcc ctcggccagg gatcagtgca
     1981 atgttttgca tcgtacgtca agaaaaagga tgacattgca ctgaacgcca tgtcggccgg
     2041 ctggatgaat gaatgggtgg aagtcgtgct gggtgcatcg attatcattc cgatctctat
     2101 tggctacctg ggtatcgacc gggtaataga actcaccaat ctgggaggtc tcgggctggg
     2161 attcagaacc atgccatacc ttttccagca atgggggcct atcctcgcag ctatggcagg
     2221 cattgccttc ttcgggttat tattcttcgc tggaattaca agctctctgg ctatgggtac
     2281 cccaatcctg tcatttatgc aggatgagtt taactggtca agaaagaatt ccgcacttgt
     2341 tttcggcggc gctgtcactg ccctgggcct tccaacagtg tttttcttcc aaaaaggtgt
     2401 tctcgatgag tacgactatt gggcagggac cgtctcattg gtcgtattcg ctatgctcga
     2461 gatcatcctc ttctcctgga tctttggaat taaaaagggc tggaaagaga taacggcagg
     2521 cgctgacata cgaataccgg gaatctacaa attcattatt aaatggatca ctccgttcat
     2581 ccttattatc gtgttcttag gctcaatggt caggccggtc aacgatgact ggacaaaagt
     2641 ttctctccga cactggccgc ttcataaaga aagtatcatc ggccagctga tccacaagga
     2701 catcgggcca aataaagctt acttcaccga tgcgttctat gcagagtttg aaggaactgt
     2761 tgattcaata agcagtatga accagaaaac cattttgcag gtttcttcag tcgatcaaac
     2821 caggtcggag acctacatac tcggcaagaa aactgaaccg gcagttaaaa cgggcgatca
     2881 gttccaggcc ggcgatactt tatatacagg taaagtggtg aacaggatcc tgtataccga
     2941 cctggcgcga ctcctcctgc tgtcgctctt tcttttctcg tccgtattgg tttacctggc
     3001 tgccgtaaaa cgaaaaaaat atgccgatcg tcaccattaa cacgtaaaac tatgaatacc
     3061 tcagcaatta tatttatggc gctggcacaa acgatcatta ctttgatcac cttctcattc
     3121 ctgtaccggg caatgagagc taaaaaacgc cctgagcctg attcttatgc agacaatgat
     3181 gacgatgatg atgacgagaa ttaaattcgt cagtcttaaa gatcacagag tatcatcctt
     3241 cgtaaactat tgtatataag ttacttgtaa ttttcttctt caccgaccgg gtatttttta
     3301 ccctttttcc ggattaatca gtatgataaa ccggatcaaa gattactttt catttactgc
     3361 gaaagaacag cggggcctta tcgccctgct ggggattatc ctgttggccc tgtcagccgg
     3421 gatttacctg ccggtaataa ttcctcagaa acaatttgat attgaaccct ttagaagcga
     3481 agtcgaagcg tttcttgcaa aggctgtgat cgaagactcc tcagccattt caaaccaaaa
     3541 tcaagcttta aaattcactc cggtatcgga tcatcaggac cttgaaggtt ttcagcagtc
     3601 accttacccg ttcaatccta atgagctcga tgaaagccaa tggaagaata tgggtttgag
     3661 ggatagaatt gttaacaata tcatgaacta caggaagaaa ggcggacggt tcagggataa
     3721 ggagggattc aggaaaattt atggccttac agacaaagaa ttcggtattc ttgaatctta
     3781 ccttgttttt gatcctccgg taaaatctga atacccaaaa gacactgctt caaagcctga
     3841 cggatttcac gatcaatata caaagaaaga tttcctgccc atcagggtgg agatcaacag
     3901 tgcagattcg gctgctttgc tgaacctgaa aggaattgga ccctcttttg ccggcaggat
     3961 cgtaaaatac cgctccagac tgggaggttt ttataagccg gaacaactga tggaaataag
     4021 aggtctcgac agcgtccgat ttaaccaatt ccgtgaccag atcgagttaa actctggttt
     4081 gatctcaaag attgatatta ataatgtaac tttcaaagag ttactaaagc atccatactt
     4141 tgagtactac ctggttaaag ccatttttga gaaaaaagat cagattaaat catttgattc
     4201 agtagaacaa ataaaatacc tgccggtgat gtatgaagag ctctttgaga agatatcgcc
     4261 ttatataaca gtcaattaac atttttattc ggacgataaa tatgcctgat cttatcgatt
     4321 tcttcatcag tgatggaaac attccaaaaa tcatctcctt gtctcccggt ctcctggtct
     4381 ccgtgtctcc cggtctcctg gtctccgtgt ctcctggtct cctggtctcc gtgtctcccg
     4441 gtctcctctt cctcctccag ttcccattcg taaactttcc gttgaggccc ctgttttcta
     4501 tagtaaacag ccaggataat accggcaatc attccataaa gatgagcttc ccaggagata
     4561 ttcttttccg ggaagaattc gggaaaaatc ccccagatca ggcttccata aagaaaaaca
     4621 acgatcaggg cgagagccat caaaccggta tgcttccgga taatcccgct cagggcaata
     4681 aaagtggcaa ggccatagac aatcccgctg gccccgatat gaaaagccgg ccgggcagct
     4741 atccagaccc aaaatccact gaatagccag ataaacagta atattctaaa tgacaggtct
     4801 ttgtaaaaat aaaataacgc cgatcccagg atgatcaatg gtactgaatt ggcaataaga
     4861 tgggaaaaat cgccgtgtat gaaaggtgtt gtgatgatac cggtgagatt ctgccaccgt
     4921 aaagggaata tccccagaaa ggacaatttg taatctcccg taacctcgat gattttaaca
     4981 gcccacatca gcaaaaccag catgaaagga atcaccatgc ttctcagtat ttttcgctcc
     5041 tcctgtctga tcatatctga aaatttccgg ttgtgccagc acaaatcagg ccacttgact
     5101 tggctaagtt agttatttga tggatttcat tttcttttta aggtgccggt tttttcaaca
     5161 tccaggaaaa caggcatgtg atcactgaat ccgccatggt aacccggccc aaggtaggtc
     5221 cggaatggtt tcattcccga ataggcatta tccggttcaa gcagaaattc agccataaag
     5281 atgcctgcca tttggtcaga gaccctcaaa ccggaatcac ccgaaaagag tgcgcatgaa
     5341 accaggatct gatcgaaaac cgaccaaacc ccctggtgct tgatcgtgcc tctaccaaaa
     5401 atgttctttt ccaggctact caggtttacc agcgggcaaa atgaatcctg cagactgttt
     5461 tccaggattt tgttgaccga ctgcatgctt tcatcatccg gttcatcatt aaaatcaccc
     5521 atgacaatta tgtttgcatt ataattgctt ttaaggacct ttacgatcga ttcggacaaa
     5581 atggccgccg atgccatcct tttttgcact gaagttccgg ccccgccgta tcttgacggc
     5641 cagtggttaa tataaatatc cagcgtatcc ctgtcccaga tccggccctt tacgtggagt
     5701 atctcccttg tcgtttcacc cgatctcaaa ggtatggtca gccattctga agtgatcggg
     5761 aaaaacaggt cagcccggta gatcaaagcc acatccacac ccctggcatc atgcgactct
     5821 ctgtgtatca accggtaatc cttaaggctt aggggtgtat cataaataag cctgttcagc
     5881 acaagcctgt tttccacctc acataatcca ataacggcgg gcatatcccc ttgccctaat
     5941 gcaacaagag tcctgtaaat cttaatggtc ttttcccgga acctgtcata agtccagttt
     6001 ttatcccctt ccggcaaaaa ttcttcatcg tttgtcaaag tatcatccat gacatcaaat
     6061 aaattttcaa cgttataaaa gacaatacgt gccgattgtc cctggattcc ggttgttaat
     6121 cccatcagga tcaaacaaac ccaattacaa aataaacttt tcatctgatt tcctttttcc
     6181 tattaatccg gtttttggga tgaaaatacc cggtcggtga aaaaaatatt cggaaagtgc
     6241 aactatttgg ccttttattt gtcattatcc tgatcttttg aatcatcata aaatgacaaa
     6301 ggcacatttc agccaaattt caagcttact aacaggcatt atctttctga cctgcctttt
     6361 ttttaatgcc actgccggca ttgatccgga cgatccatcc gtgaaacggt atacgatcag
     6421 cgggcatatc aaggacaata ccagcgggga ggaccttttc ggctcttctg tcctggtcac
     6481 cgaacaaaat accggcacga ttgccaatga atatggattc tactcggtca gcctgaccaa
     6541 agggatatat acgcttcgct tttcatatat gggatatgag acccagacac acaagatcac
     6601 tgccgacaaa gacatagtcc tggatattaa tctcaatcca tcgtctgcta tattgaatga
     6661 agtggaaatc agggctgaac gcggcgatgc caatataaaa acgccggaga tgagcgtagt
     6721 taagctggat gtcaaaacga tcaaccggat tccggcactg atgggcgaag tggatattat
     6781 caaagcgata cagcttcttc ccggggtaca atcggtgagt gaaggttctt cgggttttag
     6841 tgtaagagga ggaagccccg accagaacct ggtattgctg gatgaggcta cggtttataa
     6901 tccctcccat tttctcggat ttttttcagt attcaataat gatgcgataa aagatgttaa
     6961 actatataag ggcgacatac ccgcacaata cggaggaagg ctggcttcgg tcctggatgt
     7021 gcgaatgaaa gacggaaacc agaagaagtt ttccggaacc ggagggatag ggcttatttc
     7081 cagcaggtta acgcttgaag ggccgataat taaagataaa acctcattta tcctgtcggg
     7141 tcgaagaact tatgccgatc tctttttgcc cttgtcaaaa aatgaaggag ttcaggacag
     7201 ccggctctat ttttatgatt ttaatgccaa aatcaatcat acttttaatg agaacaaccg
     7261 gttatatcta tccggttatt tcgggaatga catctttaag aatccttttg cctatatgaa
     7321 actggggaac acaacggcta ccatccgttg gaatcacctc ttttcaaaaa ggctcttcag
     7381 caatttcact gcaatatact cccgctacca gtatggttta ggaacggctg atgacgacaa
     7441 tccgaactca ttcctttgga cttctgtttt acaggattac caggtcaaag gagattttac
     7501 atattatccg ggaacaaacc atacgcttcg cttcggattc accagcagcc tccatttgct
     7561 cgatccggga agtgccagcg gaagaggcga ctcaagcctt ttcacggagt attcaatcga
     7621 ccgtaaccat tccctggaga gtgggttata cgtaagcaac gaccagttga tcggcagccg
     7681 gctgactttg aaatacggca tcaggttttc tctctttcag aacattggtc catcaaccgt
     7741 ttttctatat gatgatgaat ttcatctgag cgattcaacg aaatatgaac atggaaagtt
     7801 ctataataca tataccggcc tggaaccccg tcttgggctg acatatgtcc tggacgaatt
     7861 ttcatcggta aaggccagct atacgcatac atatcagtat ttgcaattag cacagaattc
     7921 aactgcgggt acgccgctcg atgtctggta ttccgccagt ccgaatgtaa agccgcagat
     7981 ctccgaccag gctgcaattg gttatttccg gaacttccgc aaaaacacta ttgaaacttc
     8041 ggtagaggta tattataaga ccatgcgcaa tacgatcgat tttaaagatc acgctgaact
     8101 tttactaaac aaacaactgg aaggggaact gcggttcgga aaagggtggt cgtacggagt
     8161 agaactgatg gtctccaaac cgcaaggcaa aatcaccggt tgggtaagtt acacgttctc
     8221 acgcacctgg agaaagtttg aagacattaa tgacgggaag aaatatccgg ccccgtacga
     8281 ttccccacac gatgtatcca tcgtcctgaa ttatgatgcg acaaaaagga tcagcatatc
     8341 agccaactgg atatattcta ccggcaaacc ggtaactttc ccaacaggaa gagccgtgat
     8401 cgatggcgtg gtagtgccga tttattctga tagaaatgcc taccgcatga tggattatca
     8461 ccgtcttgac ctttcggtag cactgcataa aaagccgtct gattcacgtt tcgactggga
     8521 tctggtattt tcggtctata acgcgtataa ccggcataat acatgggcca tcaattttgt
     8581 tcaggataac ctgtatcctt tgacaaccta tgcagagcaa acgtatttat tcgggatcat
     8641 tccggctgta accttcaact ttcaattctg atgatgaaaa gaatccatat cctctatctt
     8701 ctgatcccgg tgattttatc ctcctgtaca gagcggattg atattgacct ggatgaaacc
     8761 tatacccgcc tggttgtgta tggcgtactc acaaccgaca caacaacgca ctatgtaaca
     8821 ttatcaaaaa caacaagcta cttttataac gaacttcctc ccccggtttc aggagcagaa
     8881 ataattatca gcgatgacca gggaaatgaa ttcacccttc aggaaaccga accgggaaag
     8941 tatgccactt cacctttatt tgccgctgtg cagggccgga cctatacctt aaacatcagc
     9001 cttgaagaag aaatcaataa tttcagaaat tataccgcct caacaaaagt cacatccatc
     9061 aatccgatcg actcgatcgg gctggtgcat caacctttat ggggcgaaaa aggattttat
     9121 gaggtgacct gctattacca ggaaccaccc acacgcgact tttatatgtt caatatcctc
     9181 ataaacggaa aactggtgac cgataccatc aacagtagga ttgtcattaa tgatgatttc
     9241 tttaatggga attataccaa cgggatcggt gtgggatacc tcgaccagtc aaaaacggat
     9301 gaaattttac ggcccggcga caaggtgacc ttccaggga
//
LOCUS       DAOPNH010000126         9303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8112_length_9303_cov_6.185770, whole genome shotgun sequence.
ACCESSION   DAOPNH010000126 DAOPNH010000000
VERSION     DAOPNH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8112_length_9303_cov_6.185770"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PKZ74_07970"
     CDS             <1..616
                     /locus_tag="PKZ74_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92734.1"
                     /translation="FEGDDKILTLHRSGVYKLYNYDLSTHFDEDMLFILKFDPQITVS
                     AVYFDGNTGTYYLKRFAIEPSDKAVGFLNEHKDTRLEELSIDWLPRLQLAFLEKNGKQ
                     RPEEIIDVAGFIGVKSYRAKGRKLSEHAIGQIRWLDPLPYEPSGEEDNGVEQKEEDVV
                     SFEILPAAKPPEEEPEPRKPIPPESGNDEHKPPESGKQITLEFF"
     gene            646..1878
                     /locus_tag="PKZ74_07975"
     CDS             646..1878
                     /locus_tag="PKZ74_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPM92735.1"
                     /translation="MQPDNKVHDVSPSVKWCGVLDHDIVTFDVVMETKHGTTYNSYFI
                     DAERKALIETVKEGFWDVYLEKLRQITNPEEIDYIILNHTEPDHSGSLAHLLKLAPGA
                     TVVGTGNAIRYLKEMIYTDFPHIVVKDGYTLDLGNKTLQFISAPNLHWPDTMYTYLAE
                     EKILFTCDSFGAHYCHEGVFDDKVGDFDEDFRYYFDVILKPFSKFMLKAIEKVNALDI
                     NLIAPGHGPVLRSGWKKRVELSEKWAKEHLDLPVSRKVFIPYVSAYHKTGKIAEKIRD
                     GIISAGNLEAEILDIEMTSIGDLEQKVAESAALIVGSTTINQNILLPVYKLFSVINPL
                     RDKGKLAGGFGSYGWSGESKDLIKNNLENLKLNYFGEGVFVKFTPSPVEEQQAFAYGK
                     AFGEELLSGTNAGTKSEG"
     gene            1875..2714
                     /locus_tag="PKZ74_07980"
     CDS             1875..2714
                     /locus_tag="PKZ74_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92736.1"
                     /translation="MRTLRSAILLIFIAALFTGCTLEQRLASSFVKLDPKPQVYLLEP
                     DHIFKYNLKEFELSGSDTMDQVVKDSLLLEKSLFLNTFSDSVVIERLMAGMISGLKRS
                     GLSIIPESDMDSLLGQGGNPFILNLAQFTLEEYIHPYRSEEEVYDEIIVIDGIDVNAI
                     NFNLWIELGIMNSEMKNRVLFYSDFLMDKISGTLKQNLMTGKMSFDYSIDTISPGWIT
                     EFAYKFGTVSASYFYDYLLNEYIGEKLPDNYPYERYYYHFDPERRLIYPVDESERLTE
                     IKE"
     gene            complement(2721..3725)
                     /locus_tag="PKZ74_07985"
     CDS             complement(2721..3725)
                     /locus_tag="PKZ74_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPM92737.1"
                     /translation="MKAIILPEYNPNVIRALKSLHVEETDIPKPAGRNVLVKVAASPC
                     NPSDIAFMRGSYNIRKPVPAVMGFECTGTVVETGDHREAKALLGKRVSCFSQSDENGT
                     WAEYFLTDFNNCLTIREDLDDDQASALCINPFTAFALFSMALDRKANAIIQNGSSGQI
                     GIFIRTLARRNRIPVINLVRKDDHIKVLQDAGEKHVLSMNDPDFESGLAAIAREVRAT
                     IAFDAVGGDISGTIINAMPPGSELVVYGGLSGKPIGMVNPLEVIFKSKSIRGFNLGDW
                     KIEIGSKRFQEISDELQQLMVSRILVTRIQCAFSLEEVHQALEQYIRNMSSGKIIFQP
                     "
     gene            complement(3722..4750)
                     /gene="tsaD"
                     /locus_tag="PKZ74_07990"
     CDS             complement(3722..4750)
                     /gene="tsaD"
                     /locus_tag="PKZ74_07990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPM92738.1"
                     /translation="MSIYILGIESSCDDTSAAVIRDREMLSNLIANQDVHRAFGGVVP
                     ELASRAHQQNIIPVVQQALDRAKIDKNQLHAVAYTRGPGLLGSLLVGTSFAKAFALSL
                     GIPIIDTDHMEAHILAHFLMEPGVEKESPDFPFLCLTVSGGHTQIVLVKDYFDFTIVG
                     RTIDDAAGEAFDKAAKIMGLPYPGGPLIDKLAKEGDENAFRFPMPSIPGLDYSFSGLK
                     TSFLYFLRDHLKTDPGFIENHKADLAASIQKAIIKTLMKKLLKAASELNIHDIAIAGG
                     VSANSRLRNELLELRETKGWRVFIPKFEYCTDNAAMIAITGYFKYLKGEYGRQDAPVY
                     ARSNMVNL"
     gene            4800..9209
                     /locus_tag="PKZ74_07995"
     CDS             4800..9209
                     /locus_tag="PKZ74_07995"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPM92739.1"
                     /translation="MIAVVVLIMTAITLYSLLRTPSLQQFAGRLASGFLTERLGHEIR
                     LESLRISDFIYVETRGIEVRDTLDNVILGIENLRVKIDDFSLGRHRLSFERVSIDSGT
                     FAMVYFNDDSLSNLGYFIRKFTRSDTTVTDTTPAPAWHISCSNLLIDRFTYSLKREGV
                     EAGEQDGVNFRDLGIHDISIDMREIAVIGDSISAYIDHLACRENSGLELLNFSGDATV
                     YSKGIIVDGAQISTGRTSLDLDLAFRYDGYDRLSEFLDSVRIEADIRSSLITPGDIGY
                     FVPALAGMDDPVMFSGKVNGPVSDFRATSLSVNLGDFTLFEGDLAINGLPDFDSAFID
                     LSIDQFASTPGEIANLKFPAYDLASVIPDELNTLGYTSLQGNFSGTPHDFRIDAMVRS
                     EAGNATLNGKYTEDRPNEAPNFRLKVEVLSADLGNLLSSDDLGIVNLQAELNGSGSSL
                     NDLKVDLNALVTGLEYRNYRYNEIVIDGTVTGKSFSGRLDVNDPNFKAAYEGTVDMNE
                     DLPDLDFKLTVDKARLYETGLTTRSEDAMISGGLTVDIEGWNAEYFRGDIRVDNLHYL
                     ENGRHYTLKMLNVSRGRQEEKIDILTVRSDYIDADMAGKFNIRDLYRQVYGFIRNDES
                     DSLIFQEMENNPQVADMEVRIKDINPITELFVPGMHTSPGIMLSGRFSSVDRILEAEG
                     DIRELIYSGMSADTIRLFAVYAFGSLYLDAGMRHLNLSEDQENEGIGIENLQLHTALT
                     GNDLYYAVQWNNDDPLKTNAGEFEGYARLLPDGAVETGLRNAQANINGHQWMISDLNI
                     IRMDSASVGVKNFEIYGANEHFIIDGVLSHSAEDTLSLYFRNWELANFNPFLENYSLA
                     LGGEINGHFGLFRHERKINVFSALNVNDFTLNEVYLGEMDFDTRWIQSERALAVDLNL
                     FSEGNTNEHFKILGVNGVYYPMDKKRNFDFDIIAKNLDISVLKPFLTSFSSKLAGYAT
                     GRLKLAGTNADPVVMGRLRLQRAEMNVDYLNVTYSFSNEVVFTENGILFNELQAYDPN
                     SQKALISGGIYHDHFRDMQMDITILPDQFMAINLNRYQNEVFYGKAYATGTVHLSGPF
                     DNLSISADVLTEKGSAITIPINYSVDVSENDFIIFSDSRDTVVDDNNREKQVSGLALD
                     LGMNITKDADIEIILPGNIGSIAANGDGNLRLGVDPYGYLNLTGSYVIRSGLFVFSLE
                     QLVSRRFEILEGSSISWTGDIYDAEVSISARYRLRTDLNGLGISMIDPDAANQKVIVN
                     TDIRMSGNLFNPDLKFGITFPNMQEQTRQAVYAVLDTNDLGLMNQQAISLLVLGSFSS
                     TGTGGSNLVNPASIVSNTLSNMLSQINNDFNIGINYMPGDQVSAEQLEVALSTQLLDD
                     RLIIDGNIDVSGSNANTQKTSSIVGDINIEYKLSPDGRFRVKAFNRSNDLSLFNDYAP
                     FTQGIGVFYRKEFDNLRDLFTPRQPKEKKVTRE"
BASE COUNT         2486 a         2055 c         2292 g         2470 t
ORIGIN      
        1 ttttgaaggc gacgataaga tcctgactct tcatcggtca ggcgtctata aactttataa
       61 ttacgacctg tcgacccact ttgatgagga tatgctcttc attctgaaat tcgacccgca
      121 gattaccgta tccgccgttt atttcgacgg caataccggc acatactatt tgaaaaggtt
      181 tgctattgag ccttcggata aagcggtcgg ttttctgaat gaacacaaag acacccggct
      241 ggaagagctg agtatcgact ggctgccccg gttgcagtta gctttccttg aaaagaacgg
      301 aaaacaacga ccggaagaaa tcattgatgt ggccggtttc ataggggtga agagttaccg
      361 tgccaagggc aggaagctgt cggaacatgc catcgggcag atacgctggc tcgatccgtt
      421 gccctatgaa ccgtccgggg aagaggataa tggcgtagag caaaaggaag aggatgtcgt
      481 ttcctttgaa atccttccgg ctgcgaagcc tccggaggaa gaacctgaac cccggaagcc
      541 aattccgccc gaatccggga atgatgaaca caaacctccc gaatccggta agcagatcac
      601 cctggaattt ttttaatcat aacagaggaa aagtaaaata atgctatgca acccgacaac
      661 aaagtacatg atgttagtcc ttcagtgaaa tggtgcggcg tgcttgatca cgatatcgta
      721 actttcgacg ttgttatgga gacaaagcac ggtacgacct ataattctta ttttatcgat
      781 gctgaaagaa aagcccttat cgagacagtc aaagagggat tctgggatgt ttatctcgaa
      841 aagctcaggc agataactaa ccccgaagaa attgattaca tcattctcaa tcacaccgag
      901 cctgaccatt caggaagcct ggcgcatctg ctcaaactgg cccccggcgc cacagtggtc
      961 ggaaccggga atgctatccg ctatctgaag gagatgatct ataccgattt cccgcatatt
     1021 gttgtaaaag acggttatac actcgacctg ggcaataaaa ccctgcagtt tatcagcgcc
     1081 cccaacctgc actggcccga taccatgtac acctacctgg cggaggaaaa gattttattt
     1141 acctgcgatt catttggcgc ccattattgc cacgaaggtg tttttgatga taaagtggga
     1201 gactttgatg aagatttcag gtattatttt gatgtcatcc tgaaaccgtt cagcaaattt
     1261 atgctgaagg ctattgagaa agtcaatgcc ctggatataa acctgatagc cccgggtcac
     1321 ggaccggttt tgaggtcagg atggaaaaaa cgagtggaac tttcagaaaa atgggcgaaa
     1381 gaacacctgg atcttcccgt tagccgcaaa gtctttatcc cttatgtatc tgcttatcac
     1441 aaaaccggta agattgctga aaagataaga gatgggatca tttcggcagg aaacctcgaa
     1501 gcagagatcc tcgatatcga aatgaccagt atcggcgacc tggaacaaaa agttgcggag
     1561 agtgccgccc tcattgttgg gagcacaacc attaaccaga atatcctgct tccggtttat
     1621 aagcttttct cggttatcaa cccgcttcgc gacaaaggca agctcgctgg aggttttggc
     1681 tcttacggct ggtcgggaga gagtaaagac ctgattaaga ataacctgga aaacctgaag
     1741 ctgaattatt tcggcgaagg agtctttgtg aaattcaccc cgagcccggt tgaagaacaa
     1801 caggccttcg cttatggaaa agctttcggt gaagaattgc tctcgggcac aaatgccggc
     1861 acaaaatctg aaggttgaga acgttaaggt cagccatact attgatcttc attgcagctt
     1921 tgttcaccgg ttgcaccctc gaacaaagac tggcttcctc ttttgtaaag cttgatccga
     1981 aaccacaggt ataccttctg gagcctgacc acatctttaa atataacctg aaagagtttg
     2041 aactttcggg ctccgacacg atggaccagg ttgtcaaaga cagcctcctt cttgaaaaga
     2101 gtttgttttt gaatactttc agtgattcag tggtcatcga aagattgatg gccggaatga
     2161 tcagcggtct taagaggtcg ggcttgagca tcattcctga aagcgatatg gattcattgc
     2221 ttggccaggg ggggaacccg tttatcctca accttgccca gttcactttg gaagaatata
     2281 ttcatccata caggagcgaa gaagaggtat atgatgaaat tattgtgatt gatggaattg
     2341 atgtaaatgc gatcaatttc aacttatgga tagaactggg aattatgaac tcagaaatga
     2401 agaacagggt ccttttttat tctgatttcc tgatggataa aatatccgga accctcaaac
     2461 aaaacctgat gaccggcaag atgagctttg actattccat cgatacgatc tcgcccgggt
     2521 ggataacgga attcgcttat aaattcggaa ccgtctcggc atcttatttt tatgattacc
     2581 tgttgaatga atatataggc gagaaacttc ccgataacta tccctacgaa aggtactact
     2641 atcattttga tccggaacgg aggctgattt acccggtaga tgagagcgaa cgacttaccg
     2701 aaatcaagga atgagatgca tcagggctgg aaaatgattt ttcccgacga catgttgcgg
     2761 atgtactgtt cgagtgcctg gtgaacctcc tcgagtgaga aggcgcactg gatacgggtt
     2821 acaagtatcc gtgataccat cagctgctga agctcgtcac tgatctcctg gaatcttttg
     2881 ctgccgattt ctatcttcca gtcgcccagg ttaaatcccc tgatggattt tgatttgaat
     2941 ataacttcaa gaggattgac cattcctatc ggttttccgg ataatccgcc ataaacaacc
     3001 agttcggagc ctggtggcat ggcgttgatt atggtacctg agatatcccc cccgaccgca
     3061 tcaaatgcaa tggtggccct tacttcacgg gctattgcag ccagaccgga ttcaaaatca
     3121 ggatcgttca tgctgagcac atgcttttcc ccggcatcct gcaaaacctt gatatgatca
     3181 tctttacgca ccaggttaat gaccgggatc cggttacgcc gggccagggt tcggataaag
     3241 attccgattt gccctgaaga gccattctga ataatggcat tagcctttct gtcgagagcc
     3301 atgctgaaca acgcgaatgc ggtaaacggg ttgatacata gcgcggaagc ctgatcgtca
     3361 tccaaatctt ctctgatagt caggcagtta ttaaaatccg tgagaaagta ctccgcccag
     3421 gttccattct catccgactg ggaaaagcag ctcacccttt ttccaagcag cgcctttgct
     3481 tcgcgatgat ctccggtctc aaccacggtt ccggtgcatt caaaacccat taccgcgggc
     3541 accggtttgc gtatattgta actgccgcgc ataaaggcaa tatcggaagg attgcagggc
     3601 gaggcagcga cttttaccag gacattccgg cccgcaggct ttgggatatc ggtctcttcc
     3661 acatggagac tttttaatgc ccggatcacg ttggggttgt attcaggaag aataattgct
     3721 ttcataagtt caccatattg cttctggcat ataccggtgc atcctgcctg ccatattctc
     3781 ctttgagata tttaaaataa ccggtgatgg cgatcatggc ggcattgtcg gtacaatatt
     3841 caaattttgg aatgaagact cgccagcctt tcgtttctct cagctccagc aattcgtttc
     3901 tgagcctgga gttggctgaa acaccccctg caatggcaat gtcatggatg tttaattccg
     3961 aggctgcctt gagcagtttt ttcatcaaag ttttgataat ggctttctgg atggaagctg
     4021 ccagatcggc tttgtgattt tcgataaacc ccgggtctgt tttaagatgg tcgcggagaa
     4081 aatagaggaa ggaggtcttt aatccgctga aactgtagtc gagaccggga atagaaggca
     4141 taggaaagcg aaatgcattt tcatccccct cctttgccag tttatcgatc aacggcccgc
     4201 cgggataggg gaggcccatg atcttggccg ctttgtcgaa cgcctctccg gctgcgtcat
     4261 cgattgtcct tcccacaatc gtgaaatcga aataatcctt tacgagaacg atctgggtgt
     4321 gccctcccga taccgtcaga cagagaaaag ggaaatccgg ggattctttt tccacccctg
     4381 gttccatcag gaaatgggcc agaatgtgcg cttccatgtg atcggtatcg atgatcggga
     4441 tcccaaggga aagggcaaat gctttggcaa aagaggtgcc aaccagcaga gatccgagca
     4501 atcccggccc ccgggtatag gctacggcat gcaattgatt tttatctatc tttgccctat
     4561 ccaaggcttg ttgaaccaca ggaatgatgt tctgctggtg ggctctcgag gcaagttcgg
     4621 gaacaacgcc gccgaatgcc cggtgcacat cctggttggc aatcaggttg gataacattt
     4681 cgcggtcgcg gatcaccgcc gccgaagtgt cgtcgcagga ggattcaata ccaaggatgt
     4741 aaatgctcat aaatcttatt cttacgggca aaattattaa aaatatcctt aaggcgtgtg
     4801 tgatagctgt cgtcgtgctt atcatgacag ccatcacctt gtattcattg ctgcgtaccc
     4861 cttccctcca gcaatttgcc gggcgactcg catcaggatt tcttacagag cgacttgggc
     4921 atgaaatccg gctggagagt ctgaggatca gtgactttat ctatgttgag acccggggta
     4981 tcgaagtcag ggataccctt gacaatgtaa tccttggtat tgaaaacctc cgggttaaga
     5041 ttgatgattt ttccctcggc cgccaccggc tctcgtttga aagggtgtcg attgacagtg
     5101 gaactttcgc tatggtttat tttaatgatg acagcctgtc gaacctcggc tattttatca
     5161 gaaagtttac ccggtctgac acaacagtta ccgacactac gccggcacct gcctggcata
     5221 tttcctgcag caacctgctg atcgaccggt tcacctattc cctgaagcgg gaaggagtgg
     5281 aggctggaga gcaggatggt gtgaacttca gagacctggg tattcatgat atttccatcg
     5341 atatgagaga gatagcggtg attggcgact ccatctcggc ctatatcgat cacctggcct
     5401 gccgggagaa ctctggtctg gaactgctga atttcagtgg agatgcaaca gtttattcta
     5461 aaggcataat tgtcgacggg gcgcagatct ctacaggccg caccagcctc gaccttgacc
     5521 tggctttccg gtacgacggt tatgaccgtt tatccgaatt tcttgacagt gtacgaatcg
     5581 aggctgatat cagaagctca ctgattactc ctggagatat tgggtatttt gttccggcac
     5641 tggccggtat ggatgacccg gtgatgtttt cgggtaaagt gaatggcccc gtcagcgatt
     5701 tccgtgcaac aagcctgtcg gttaacctcg gcgattttac tttgtttgaa ggcgatttgg
     5761 ctattaatgg attacctgat ttcgattcgg cctttataga tctttccatc gatcagtttg
     5821 cttcgacacc gggagaaatt gccaatttaa aattcccggc gtacgacctg gcatcagtta
     5881 taccggacga gttgaacacc ttgggctata cttcattgca gggaaatttc tcggggaccc
     5941 cacatgattt caggatcgac gccatggtaa ggtccgaggc cggaaatgca acattgaatg
     6001 gcaaatacac tgaagaccgg cccaacgaag caccgaattt tagactgaaa gtggaagtgt
     6061 tatctgccga cctgggcaat ctgttgtctt ctgatgatct gggcatagtt aatttacagg
     6121 cggaattaaa cggttcgggt tcctctttaa acgatctgaa agtcgacttg aatgcactgg
     6181 tcacagggtt ggaataccgc aattaccggt acaatgagat agttattgat ggtacggtga
     6241 caggaaagtc attttcgggc cggctggatg tgaatgatcc gaattttaaa gcggcctatg
     6301 agggaaccgt ggatatgaat gaggatctac ccgatcttga ctttaaactg actgtagata
     6361 aagcccggtt atatgaaaca ggactgacaa cccggagtga agatgcaatg atctcgggtg
     6421 gattgacggt cgatattgaa ggttggaatg cggaatattt tcgtggggat attcgggtgg
     6481 ataacctgca ttatctggaa aacggcaggc attatacgct gaaaatgctg aatgtgagtc
     6541 ggggaaggca ggaagagaag atcgatatcc tgacagtcag gtccgattat attgatgccg
     6601 atatggcggg taagttcaac atccgggatt tataccggca ggtgtacggc tttatcagaa
     6661 atgatgaatc agacagcctc atttttcagg agatggaaaa taatccccag gttgctgaca
     6721 tggaagttcg gatcaaagat atcaatccga tcactgaact ttttgttccc ggaatgcaca
     6781 cttcgcccgg catcatgctg agtggccggt ttagttcggt ggaccggatt cttgaagcgg
     6841 aaggcgatat cagagaactt atttatagtg gaatgtcagc cgacactatc cggttatttg
     6901 cagtttatgc ttttggaagc ctttacctgg atgcaggtat gcggcacctg aatctttctg
     6961 aagaccagga gaatgaagga attggtattg aaaatttgca gttgcacaca gctttgacgg
     7021 gaaatgatct gtattatgcg gttcaatgga ataatgatga tccgttaaag acaaatgccg
     7081 gcgagttcga gggctatgcc cgtttattgc ctgatggggc cgttgaaacc ggattgcgta
     7141 atgcccaagc caatatcaat ggccaccaat ggatgatatc tgatctgaac attattcgga
     7201 tggattcggc atcggtaggg gtcaaaaact ttgaaatata cggtgcaaat gagcatttta
     7261 ttattgacgg agtactttcc cattcggcag aagatacact cagcctttat ttcaggaatt
     7321 gggaactcgc gaacttcaat cctttcctgg aaaactattc ccttgcgctg ggcggggaga
     7381 tcaatggcca tttcgggttg ttcaggcatg aaaggaaaat caatgtcttt tcggcattga
     7441 atgtcaatga ttttacattg aacgaggttt acctcggaga aatggatttc gatacacgct
     7501 ggatccagtc ggaaagagca ctggccgtcg atcttaatct tttttccgaa ggaaatacta
     7561 acgagcattt taaaatattg ggagtcaatg gcgtatacta tccaatggat aaaaagagaa
     7621 attttgattt tgatataatt gcaaaaaacc ttgatatctc agtattaaag ccatttctca
     7681 ccagtttcag ttcaaaattg gcaggctatg ccacaggccg tttaaaactg gccggtacaa
     7741 atgcggaccc tgtcgtaatg ggccggttgc gactgcaaag agctgaaatg aatgtggatt
     7801 atctcaatgt tacgtattcg ttttccaacg aagtcgtttt tactgagaac ggtatccttt
     7861 ttaacgaatt acaggcgtat gaccccaatt cacagaaagc gctgatctcc ggcggaatat
     7921 atcatgacca tttccgtgat atgcagatgg atatcactat cctgcccgac caattcatgg
     7981 ccattaacct gaaccgttat cagaacgagg tcttttacgg aaaagcttac gcaacaggga
     8041 cggtacatct atcgggaccg tttgataacc tctccatatc cgctgatgta ttgacggaga
     8101 aaggctctgc cataacaata ccgattaatt attctgtgga tgtatcggaa aatgatttca
     8161 tcatattctc tgatagccgg gatactgtgg ttgacgacaa taaccgggaa aagcaggtca
     8221 gcgggctggc cctcgacctg ggaatgaaca ttacgaaaga tgcagatatc gaaataatcc
     8281 ttcccggcaa tattggcagt attgctgcga atggagacgg caatctgcgg ctcggtgtcg
     8341 acccgtatgg ctatctgaac ctaacgggat cctatgtgat ccggtcggga ttatttgtgt
     8401 tttcactgga acagcttgtc agccggaggt ttgagatcct cgagggaagt tcgatcagct
     8461 ggacgggaga tatttatgac gccgaggtca gtatctctgc ccggtatcga ttgaggactg
     8521 atttgaacgg actgggaatc agtatgatag acccggatgc ggcaaatcag aaagtgattg
     8581 tcaataccga catccggatg agtggcaatt tgtttaatcc ggacttgaaa ttcgggatca
     8641 cttttccgaa tatgcaggaa cagacccggc aagcagtgta cgctgtgctg gataccaatg
     8701 atctgggatt gatgaatcag caagcgatat ccttactcgt ccttggaagt ttcagcagta
     8761 cgggaaccgg tggcagtaac ctggtcaacc ctgcgtcaat agtttctaat acgctgagca
     8821 atatgttatc ccaaatcaac aacgatttta atatcgggat taactatatg cccggcgacc
     8881 aggtctctgc cgaacagctt gaagtggcgc tttcaaccca gttgctggat gaccggttga
     8941 ttatcgacgg aaatatcgat gtttccggct ctaatgccaa tacccagaaa accagctcta
     9001 ttgttggaga tataaacatt gaatataaac tttcaccgga tggaaggttc agggtcaaag
     9061 cttttaacag gtcaaacgac ttgtcgcttt tcaatgatta tgcaccgttt acccagggga
     9121 tcggcgtctt ttaccggaaa gaatttgata atttacgtga tctttttaca ccacgccagc
     9181 cgaaagagaa aaaagtaacc cgggaataat ttcctctata tttgcaaaaa aaatcagatg
     9241 cgacgactaa ttttgctgag cattaccttg cttgttatgc ctctgttggg gctggcccag
     9301 att
//
LOCUS       DAOPNH010000127         9137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8324_length_9137_cov_6.165823, whole genome shotgun sequence.
ACCESSION   DAOPNH010000127 DAOPNH010000000
VERSION     DAOPNH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8324_length_9137_cov_6.165823"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKZ74_08000"
     CDS             <1..967
                     /locus_tag="PKZ74_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92740.1"
                     /translation="VYKDGLKDNEASRKALLKMMERFPANKHELISYYYLYKTYLDDG
                     FLTEADFYKDQIIYKYPESDYARVLSDPEYYARIEAEKNKVNLLYAETFRDYKAGQYF
                     QVIAKSDLAFTLYGDTMELAPNFAYLKAISVGKIDVLDSLILHMKKIIRKYPKSEVKT
                     LAQNVLATLAEDNPEIKAETREFLPDEKKPEETLPEKASPYKVNPTGQHMFMIVADSK
                     EMRLNPFKVKLSDFNTKYFSIEDLKINSLVLDNEHYVVTVGNFSNAQKARDYFDLIVI
                     NEYVYADLKAGTFFNFVISTENYSLFFKEKDIGGYSTFFEKNYNQ"
     gene            993..1409
                     /locus_tag="PKZ74_08005"
     CDS             993..1409
                     /locus_tag="PKZ74_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPM92741.1"
                     /translation="MAKTIISESPAVNMIGKGTSIKGDIRSDGDFRVDGILQGSIHST
                     GKIVVGVSGSIEGDITCQNADFSGQVKAVIHVKELLSLKSTSKVIGEVHTSKLAIEPG
                     AKFSGTCNMEDEPEFKVQKEITQDEPVTREKEKITG"
     gene            1369..1617
                     /locus_tag="PKZ74_08010"
     CDS             1369..1617
                     /locus_tag="PKZ74_08010"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPM92742.1"
                     /translation="MNLLPGKKKKLLDNYARYSSIAFQMLVIILAGVFGGIKLDEWLE
                     MKIPVFTIVFSILSVILAIYTVTKDLIRSGKKEDGKDS"
     gene            1623..2015
                     /locus_tag="PKZ74_08015"
     CDS             1623..2015
                     /locus_tag="PKZ74_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92743.1"
                     /translation="MNKKYARFIVRLWLLTAAMAAFIAGVNVLTAPPLFPPVLYLFLS
                     LFFMVHAAVHYILLKITSLNPGKFVSYFMLSTTLKLFLYLIVLIFYVINTHQPIMVPV
                     IAFFIMYIVFTVFEVISIINQTREDRSS"
     gene            2068..2673
                     /gene="folE"
                     /locus_tag="PKZ74_08020"
     CDS             2068..2673
                     /gene="folE"
                     /locus_tag="PKZ74_08020"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPM92744.1"
                     /translation="MDDYGYKKTDNYNYKKVNAMARHYKEVLRHIGEDPEREGLLDTP
                     VRVAKSMQFLTHGYMLNPEEILRSAMFKEDYREMVIVKNIEIYSLCEHHMIPFFGKAH
                     VAYIPNGYIVGLSKIPRVVDAYARRLQVQERLTTQIKDCIQDTLKPLGVAVVIEALHM
                     CMAMRGIQKQNSITTTSDFTGAFLKEPTRAEFIHLIGSDLH"
     gene            2750..3643
                     /gene="fabD"
                     /locus_tag="PKZ74_08025"
     CDS             2750..3643
                     /gene="fabD"
                     /locus_tag="PKZ74_08025"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPM92745.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLSEGSALARELFSKANSILGFNITE
                     IMFEGTDEDLRQTKVTQPAIFLHSVILSRVLGNEFKPDLVAGHSLGEFSALVANKVLS
                     FEDGLRLVSKRAIAMQKACELKPSTMAAVIGMEDTDVERVLSLIKDEIVVPANYNCPG
                     QLVISGSVQGIETAAQKLSEAGAKRVMTLNVSGAFHSPFMQPAAEELANAINTTKFSK
                     GICPIYQNVTGQGVTDPEIIKKNLIAQLTSPVRWSQSVKNMIASGVTHFTEVGPGTVL
                     QGLIKKVKKDVVTESAEVKRS"
     gene            complement(3712..4464)
                     /locus_tag="PKZ74_08030"
     CDS             complement(3712..4464)
                     /locus_tag="PKZ74_08030"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92746.1"
                     /translation="MRISMVFGNIFSSARSSLAGFSALGTDMHSHLIPGIDDGARTLD
                     DSVNLIRSMAGLGFKKLITTPHIQHDFYRNTREIILNGLDTVRNAIRSENIPVEIEAA
                     AEYLLDDGFETLAENGDLLSFSGKHILVELSYFNPNPNLKNLVFNLQCDGYKVILAHP
                     ERYTYWFSDFGKFEDLKDRGIFFQLNLVSLTGFYPDPVKKFAEKLIDKGMYDFAGSDM
                     HNMNYMNALEKSLKEKSLKKLLESGKLRNSIL"
     gene            complement(4439..5506)
                     /gene="rfbB"
                     /locus_tag="PKZ74_08035"
     CDS             complement(4439..5506)
                     /gene="rfbB"
                     /locus_tag="PKZ74_08035"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPM92747.1"
                     /translation="MTNKLHRTIIITGGAGFIGSHVVRLFVNKYPEYRIINLDALTYA
                     GNLENLRDVEGKPNYTFVRADISNEAEVRQVIRQFHPDGIIHLAAESHVDRSISNPTE
                     FVFTNIVGTVNLLNAAREIWKDNPEGKRFYHISTDEVYGSLGDTGFFTESTPYDPKSP
                     YSASKASSDHLVRAYHHTFGLPAVISNCSNNYGPNQFPEKLLPLFINNIRQNKPLPVY
                     GKGDNVRDWLYVEDHAAAIDLIFHRGRDGETYNIGGNNEWKNIDLIRLLCRLMDDRLD
                     RPPGTSEKLIAFVKDRAGHDLRYAIDSGKLQNELGWKPSVTFEQGLARTVDWYLANQQ
                     WLDHVSSGDYLKYYENQYGVR"
     gene            complement(5584..6591)
                     /gene="galE"
                     /locus_tag="PKZ74_08040"
     CDS             complement(5584..6591)
                     /gene="galE"
                     /locus_tag="PKZ74_08040"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPM92748.1"
                     /translation="MKILVTGGTGYIGSHTVVELQLLGHEAVIVDNLSNSSAGVVNRI
                     GKITGEKPALYVVDLTDEAKTEKVFRENFNFDAVIHFAALKSVGESVQSPLKYYRNNV
                     ISTLNLLKMMTKYEIGRIVFSSSCTVYGQPDKLPVTEQTPLKRAESPYGNTKKVMEDI
                     IFDVTRAKDIHAIALRYFNPIGAHESALIGEAPSDIPDNLVPYITQTATGKRPYLRVF
                     GNDYNTPDGTPVRDYLHVTDLAKAHLAALNRMNEGKMKEKFEVFNLGTGKGYSVMDII
                     SSFEKVAEMKLNYKVVARRPGDVEQIWADPTLANTELGWKAEKTLDEMLLSAWNWQKA
                     K"
     gene            6679..7410
                     /locus_tag="PKZ74_08045"
     CDS             6679..7410
                     /locus_tag="PKZ74_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92749.1"
                     /translation="MQTNLRALSKELKNYFLDFTSETNLTSVQKTEQDNELRRVLRGK
                     GILFGEHRNKKTDQVEYYNFNLTDYFIEDTGIIRDINSSGDFPISLYLNDQINVSYEP
                     RNISKIREDYRRDISNAELQHLKMKSEFTFTGEVLNARYDAVSDKFHYLVTGNEESIA
                     DRDNQKLARKEGMKRGALGAFYGFVTGLIAVVGIYIFVGIYNMVSKYDMKAGFIVPVA
                     VTLVITAVWGYISYRKKYSEIRKWK"
     gene            complement(7413..8894)
                     /locus_tag="PKZ74_08050"
     CDS             complement(7413..8894)
                     /locus_tag="PKZ74_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM92750.1"
                     /translation="MDEERRRKLILVNPVNRVRIGFMNSEGTIAMPLGLGIVAALTPE
                     HWEVELVDEYFGEFTLKSADLVALTGFTPAAQRAYEIAAMCRQAGIHTVMGGIHASMC
                     PEEAATFVDTVFLGEAEGAWPALIDDFEAGRIKSRYDGGIVAVNDIASPRRDIFDRYP
                     YVYDLLQTSRGCPMGCEFCSVTQMCGKTYREREINAVIADLEKTRRPLLFFVDDNLVN
                     NKKGSEERAINLFKAMVDRGLTREWYSQAAVNFAANDEVLYWARKSGCYMVLMGIEAE
                     KPQALKDVRKNLNLRLGTGNYNHVFKKMHRHGIGILASMIFAMDSDSAEDLYARRDFI
                     LNSSIDTYQCTILTPLPGTILFDRMRAENKIIMNNYPSDWQQYDGMVATSRSATLERD
                     EIEQAMRRIWMDLYHKETLRKKMFRTLWNTRSFKTAYRTYATGHNYGRMFLERFIKNG
                     DGINEKLEWINKKRSIFLKITDRVIWLLYRIAWQKSSEKLNPK"
     gene            complement(8899..>9137)
                     /locus_tag="PKZ74_08055"
     CDS             complement(8899..>9137)
                     /locus_tag="PKZ74_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92751.1"
                     /translation="LWNTRSFRTAYWAYAANHNYGRMFLERFIQTDKECLNSRLEWVG
                     KKRSMYLKLTDALLWVFYQTAWKKILKRVSGNKA"
BASE COUNT         2354 a         2155 c         2292 g         2336 t
ORIGIN      
        1 tgtatacaaa gacggattga aagataacga agcatcccgg aaagccctgt tgaagatgat
       61 ggagcggttt cctgccaaca agcacgaact catctcttat tattacctat ataaaaccta
      121 cctggacgac ggtttcctga cggaggccga tttttacaag gaccagatca tttataaata
      181 tcctgaaagc gattatgcca gggtgctgtc ggatccggag tattacgccc ggatcgaggc
      241 agagaaaaac aaagtcaacc tgctgtatgc cgagactttc cgggattaca aagccggaca
      301 atacttccag gtcatcgcca agagcgatct ggcctttacc ctttacggcg ataccatgga
      361 actggctccg aatttcgctt acctgaaagc gatctcggtc ggaaagatcg atgtgctcga
      421 ctcgctgatc ctgcatatga aaaagatcat ccggaagtat cccaagagcg aagtaaaaac
      481 gctggcacag aatgttctgg caaccctggc ggaagataat ccggagatca aggcagagac
      541 cagggagttt ctgccggacg agaaaaaacc ggaagaaacc ttgccagaaa aggcatcgcc
      601 atataaagtc aatccgaccg ggcagcatat gttcatgatc gttgccgact caaaagagat
      661 gcggctcaac ccgttcaagg taaaactatc ggacttcaac accaagtatt tcagcattga
      721 agatctgaag atcaacagcc tggtgcttga caatgagcat tatgtggtca ctgtaggtaa
      781 tttcagcaat gcacagaaag cccgtgatta ttttgacctg atcgttataa atgagtatgt
      841 atatgccgac ctgaaagcgg gaactttttt taactttgtt atttcgactg agaactactc
      901 cttgtttttc aaagagaaag acatcggagg atactccaca ttctttgaaa aaaactacaa
      961 ccagtaagca tctgatttaa atttacgtta taatggcaaa aacgattatc agcgaaagcc
     1021 ctgccgtcaa tatgataggc aaagggacca gcatcaaggg cgatatccgt tccgacggcg
     1081 acttccgggt ggatgggatc ctgcagggct ccatccattc gaccgggaag atcgtggtcg
     1141 gggtttcggg aagcatcgag ggagatatta cctgccagaa tgctgatttt tcaggccagg
     1201 tcaaagcggt tattcacgtg aaggagttgc tgtcgcttaa atcgacttcc aaagtaatag
     1261 gcgaggtcca taccagtaaa ctggctatcg aacccggggc gaaattttcc ggaacctgca
     1321 atatggaaga tgaaccagaa ttcaaggttc aaaaagaaat aacccaagat gaacctgtta
     1381 ccagggaaaa agaaaaaatt actggataat tacgcccggt attccagtat tgcattccag
     1441 atgctggtga tcatcctggc gggggttttc ggggggataa aactggatga atggctggag
     1501 atgaaaatac cggtattcac gatcgtattt tcaattttat cggttatcct ggcaatttac
     1561 accgtgacca aagacctgat caggagcggg aaaaaggaag atggaaagga ttcctgaaga
     1621 acatgaataa aaagtacgca agatttattg tccgcctgtg gcttcttacc gcagccatgg
     1681 ctgccttcat cgccggagta aatgttttga ccgccccgcc cctgtttccg cctgtgcttt
     1741 acctgttcct ttcgctgttt tttatggtcc atgcggccgt ccattatatc cttttaaaga
     1801 tcacctcact caatcccggg aagtttgtca gttatttcat gctgtcgacc acactaaaac
     1861 ttttccttta cctgattgtt cttatctttt acgtgatcaa tacgcatcaa ccgatcatgg
     1921 ttcctgtgat cgctttcttt attatgtata ttgtctttac ggtttttgaa gtaatttcaa
     1981 taataaatca aaccagggaa gatcgttcct cttaatccaa ataaaatgaa aaaaaatatt
     2041 ttgaaagaga tggccgaagt caatatgatg gatgattacg gctacaaaaa gaccgacaat
     2101 tataattaca agaaggtgaa tgccatggcg cggcattaca aggaagtact gcgccacatc
     2161 ggagaagacc cggagcggga gggattgctt gataccccgg tcagggtagc caaatcgatg
     2221 cagttcctga cgcacggtta catgctgaac ccggaggaga tactccgttc ggccatgttt
     2281 aaggaagatt accgtgagat ggttattgta aagaacattg aaatttattc gctttgtgag
     2341 caccacatga taccattctt cggaaaggcg catgtcgctt atattcccaa tggatacata
     2401 gtaggtttga gtaaaatacc gcgggttgtg gatgcatatg ccaggcgttt gcaggtacag
     2461 gagcggctta ccacgcagat caaggattgc atacaggaca cgctgaaacc tcttggagtt
     2521 gccgttgtga ttgaagccct gcacatgtgc atggccatga ggggcatcca gaaacaaaat
     2581 tccatcacga ccacttccga ttttacggga gctttcctaa aggagccaac acgggcagaa
     2641 ttcatacacc tgatcggatc cgacctccat taaccagggg ataaattgcc gggtgaagaa
     2701 atttgcctac ttttggcgca cttataaaga gataatttta gtacgacgca tgaaagcata
     2761 tgtttttcca ggccaggggg cccagtttgt cgggatgggt aaggatttgt cggaggggtc
     2821 agcgcttgca agggagctgt tcagcaaagc caattccata ctcggattca atattaccga
     2881 gatcatgttt gaagggaccg atgaggacct tcgccagacc aaagtaacac agccggccat
     2941 atttcttcat tcggtgatcc tgtcgcgtgt tttgggcaac gaattcaaac ccgacctggt
     3001 agccgggcat tcgctgggtg aattttccgc tctggtagcc aacaaggtac tttcgttcga
     3061 agacggcctc cggctggtgt caaaacgggc tatagccatg cagaaggcct gcgagttaaa
     3121 accctcgacc atggcggctg tcatcgggat ggaagacacc gacgtagaac gggtactcag
     3181 cctgatcaag gatgagatcg tggtaccggc caattacaat tgcccggggc agctggtcat
     3241 ttcgggcagc gtgcaaggga ttgagaccgc ggcgcaaaag ctgtcggaag cgggcgccaa
     3301 gcgtgtcatg acgctgaatg tcagcggcgc tttccattcg ccgttcatgc agcccgcagc
     3361 ggaagagctg gccaatgcga tcaatacgac caaattcagc aaaggcatat gcccgatcta
     3421 ccagaatgtg accggccagg gcgtgaccga tccggagatc atcaagaaaa acctgatcgc
     3481 gcagctcacc tcccctgtgc gttggtcgca gtcggttaaa aatatgattg ccagcggcgt
     3541 cacccatttc accgaagtag gcccgggaac ggtgctccag gggctgatca agaaagtaaa
     3601 gaaagacgtg gtgactgaga gcgcggaggt gaaaaggtcg taagccgttc gctggtcccg
     3661 aggagttcgc gacgagggat cgttggtcat tagcaggtgc agcaggatat atcagagtat
     3721 actgttccgg agctttcccg attcgagtaa ttttttcagt gatttttcct tcaggctttt
     3781 ctccagcgca ttcatgtaat tcatgttatg catatcgctg ccggcaaaat catacatacc
     3841 tttatcaata agcttttcag cgaatttttt aaccgggtca gggtagaaac cggtcaagga
     3901 gaccaggtta agttgaaaaa agatgccccg gtctttcaga tcctcgaact ttccgaaatc
     3961 actaaaccaa taggtatagc gctccggatg cgcgagaatc accttgtatc cgtcgcattg
     4021 taaattgaat accaggtttt taaggtttgg attcggatta aagtaggata attcaaccaa
     4081 tatgtgcttc ccgctgaaac tcagcaaatc gccattctca gcgagcgttt cgaaaccatc
     4141 atccaggagg tactcagcag ccgcttcaat ttcaacgggg atgttttctg accggatggc
     4201 atttctgacg gtatccagtc cattgaggat gatctcccgg gtattgcggt aaaaatcgtg
     4261 ctgaatgtgt ggcgtggtga tgagtttttt aaaacccagg ccggccatgc tccggatgag
     4321 attgacggaa tcatccagtg tccgtgcgcc gtcatcgata ccgggaatca ggtgggagtg
     4381 catatcggtg ccaagagcag agaagccggc cagagaagat cttgctgatg aaaaaatatt
     4441 accgaacacc atactgattc tcataatatt tcagataatc gcctgaagaa acatgatcga
     4501 gccattgctg gttggcaagg taccagtcga ctgttcgggc gaggccctgc tcgaatgtaa
     4561 ccgagggttt ccaacccaat tcattttgca gtttacccga gtcgatggca tagcgcaggt
     4621 catggccggc acggtctttc acgaaagcta tcagcttttc cgatgtaccg ggcgggcggt
     4681 ccagccggtc gtccatcagc ctgcaaagca ggcggatcag gtcgatattt ttccattcgt
     4741 tattcccgcc gatgttatag gtctctccat ccctgccccg gtgaaagatc aggtcgatag
     4801 ccgcggcatg gtcttcaaca taaagccagt cgcggacatt atccccttta ccataaacgg
     4861 gtagcggttt attctgccgg atattattaa tgaaaagagg aagcagtttt tcggggaact
     4921 ggttgggccc gtagttattg gaacagttgg agatcactgc cggcaggcca aaagtgtgat
     4981 gataggccct gaccaggtgg tcgctgctgg ctttggaggc agaataggga cttttcggat
     5041 cgtagggagt ggattcggta aaaaaaccgg tgtcgcccag cgaaccgtaa acctcatccg
     5101 ttgatatatg gtaaaacctt ttcccttccg gattgtcctt ccatatctcc cgtgcggcat
     5161 tcagcaggtt aaccgttccg acaatattcg taaaaacaaa ttctgtcgga ttggagatcg
     5221 accggtcgac atgcgattcg gcggccaggt gaataatgcc atcgggatga aactgacgga
     5281 ttacttgcct gacctctgct tcgtttgaaa tatcagccct gacaaaagta taattcggtt
     5341 tgccctccac gtcgcggagg ttttccaggt taccggcata cgtcagggca tcgaggttga
     5401 tgatcctgta ttccggatat ttgtttacaa acagtcgaac cacatgcgat ccgatgaatc
     5461 cggcccctcc ggtgatgatg atggttcgat gcaacttgtt tgtcattgta gttaaggttt
     5521 gtttataatt gtcataagct cacttcgttc gcgtcagtgg ctcgctgcgc tcgcgtcagt
     5581 ggtttatttt gctttctgcc agttccaggc cgagagcagc atttcatcga gtgtcttttc
     5641 ggctttccag ccgagttccg tgttagcgag cgtcggatcg gcccagatct gctcgacatc
     5701 tccgggccgg cgtgcgacca ccttataatt gagcttcatt tcagccactt tttcaaaaga
     5761 actgataata tccattacgg aatatccttt cccggttccg agattgaaaa cctcgaattt
     5821 ctctttcatt ttaccttcat tcatccggtt caatgccgcc agatgggctt tggccaggtc
     5881 agtaacatga aggtaatccc ttaccggcgt tccgtcgggg gtattgtaat cgttgccgaa
     5941 aacccggaga taggggcgtt tgccggtggc agtctgggtg atatagggca ccaggttatc
     6001 gggaatatcg gaaggggcct ccccgatcag cgcagattca tgggcaccga tcggattgaa
     6061 ataccggagg gcaatggcgt gaatatcttt ggctctcgtc acatcaaaaa tgatatcttc
     6121 cattactttt ttagtgttgc cgtaaggtga ttccgccctt ttcaacggtg tttgttcggt
     6181 aaccggaagt ttatccggct ggccataaac agtacatgat gatgaaaaaa caatcctgcc
     6241 gatctcatat tttgtcatca ttttcagcag gttaagggtt gatatcacat tattgcgata
     6301 atatttcagc ggagattgaa cggattcccc gaccgatttc aaagccgcaa aatggatgac
     6361 cgcatcgaaa ttgaaatttt cacggaatac tttttccgtt ttcgcttcat ccgtcaggtc
     6421 gacgacatag agggccggtt tttcgccggt tattttaccg atccggttca ccacacccgc
     6481 cgaactgttg gagagattat cgacgatcac cgcttcatgt ccgagcagct gcaactccac
     6541 taccgtatga gagccgatgt atccggtacc gcctgtaaca aggattttca tagtaagagc
     6601 agttatgaaa ggcaaagata agtgaattta caaaatatca gatttcctct tatctttgct
     6661 aaactcaaac caaaacctat gcaaaccaat ctgcgtgcac ttagcaagga gcttaaaaat
     6721 tattttctgg atttcacctc cgaaaccaat ctcacttcgg tgcaaaaaac cgaacaggac
     6781 aatgaattaa ggcgagtact acggggaaaa gggatcttat tcggcgagca caggaataag
     6841 aagaccgatc aggtggagta ctataatttc aacctgaccg attatttcat agaagacacc
     6901 gggatcatcc gcgatatcaa ctcttcgggc gactttccga tctctttgta tttaaacgat
     6961 cagataaacg tttcgtatga gccccggaat atttcaaaga tcagggagga ttaccggcgc
     7021 gacatatcca atgctgaact gcagcatctt aagatgaaga gtgagttcac cttcaccggc
     7081 gaggtgctga atgcccggta cgatgcggtc agtgataaat tccattacct ggtgaccggg
     7141 aatgaagaga gtatcgctga cagggacaac cagaaactgg cccggaagga aggaatgaag
     7201 cgcggtgcac tgggcgcttt ttacggtttt gttaccgggc tcatcgctgt agtggggatt
     7261 tacatttttg ttggcattta caacatggtc agcaaatacg acatgaaggc gggattcatc
     7321 gtccctgttg cagtgaccct ggtcattacg gctgtatggg gttatatcag ctacaggaag
     7381 aaatattcgg agatcaggaa atggaagtga tcttattttg gattcagttt ttcagaggat
     7441 ttctgccagg cgatccggta cagcagccag ataacccggt cggtgatctt caggaagatt
     7501 gatcttttct tgttgatcca ctccagtttt tcattaattc catccccatt cttaataaaa
     7561 cgctccagga acatacgtcc gtaattatgt cctgttgcat aagtgcggta ggccgtttta
     7621 aaactgcgtg tattccagag ggtgcggaac atttttttac gcagggtttc cttgtggtac
     7681 aggtccatcc agatgcgccg catagcctgt tcgatctcat ccctttccag tgtcgctgag
     7741 cgggaggtgg ccaccatccc gtcgtattgc tgccagtcgg acgggtaatt gttcattatg
     7801 attttattct ccgcccgcat ccggtcgaac aggatggtac cgggcaaggg ggtcagaatc
     7861 gtgcactggt aagtatcgat ggaactattg aggatgaaat cgcggcgggc atagagatcc
     7921 tcggccgaat cgctgtccat ggcgaagatc atggaggcca ggataccaat tccatgccgg
     7981 tgcattttct taaagacatg gttgtaattg ccggtcccca gccggaggtt caggttcttc
     8041 cggacatctt tcagggcctg tggtttttct gcttctattc ccatcaggac catatagcaa
     8101 ccgcttttac gggcccagta aagaacttcg tcgttggcgg cgaaattcac tgccgcctgt
     8161 gaataccatt cccgggtgag tccccggtcg accatggctt tgaaaaggtt aattgccctt
     8221 tcttcagacc cttttttatt attgaccagg ttatcgtcga cgaaaaaaag cagcggtcgc
     8281 cgggtttttt ccaggtcggc gatcaccgcg ttgatttcgc gttcccggta ggttttgccg
     8341 cacatctgtg tcacggagca gaactcgcat cccatcgggc agccgcgtga agtctggagt
     8401 aggtcgtaca catagggata acggtcgaag atatccctcc tgggggaggc aatgtcgttc
     8461 accgcaacaa tccctccgtc gtaccggctt ttaatgcggc cggcttcaaa atcgtcgatc
     8521 agggcgggcc aggcgccttc cgcttcgccg aggaagacgg tatcgacaaa cgtggccgct
     8581 tcttcggggc acatggaggc atgaatccct cccataacgg tatggatccc ggcctgccgg
     8641 cacatagcgg cgatctcata agcccgctgg gcagccggtg taaatcccgt caaggctaca
     8701 agatcggcag atttgagggt gaactccccg aaatactcat cgaccagttc cacctcccag
     8761 tgttccgggg tcagggccgc cacgatcccc aggccgaggg gcatggcaat agtcccttcc
     8821 gaattcataa agccgatcct gacccggttg accggattga ccaatatcag ctttctgcga
     8881 cgttcttcat ccatcaattc atgctttatt accggagact cttttcagga tttttttcca
     8941 ggcggtttga tagaagaccc aaaggagggc atcggtcaac ttcagataca ttgaacgctt
     9001 cttcccaacc cattccagcc gggagttcaa gcattcctta tcggtttgta taaacctttc
     9061 caggaacatc cggccataat tatggtttgc ggcatacgcc cagtaagcgg ttcggaaact
     9121 tcgcgtattc cagaggg
//
LOCUS       DAOPNH010000128         9098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8379_length_9098_cov_6.113237, whole genome shotgun sequence.
ACCESSION   DAOPNH010000128 DAOPNH010000000
VERSION     DAOPNH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8379_length_9098_cov_6.113237"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKZ74_08060"
     CDS             <1..1271
                     /locus_tag="PKZ74_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92752.1"
                     /translation="FLNRSTGKVRTGMGAGREDVNVSCRGGSRVEIKGVAHTRWIPEL
                     THNEAFRQWALLHIRETLKQRVKNPGNWKIASKILAYHNFNFKYKPLREARNSKNRIV
                     AVNLPDFKGLMSHFTQPGKIFADEISDRLKVIACIEKPHMAHSEDLTPLVTLKDWETI
                     RKMFKAKENDAQLIFWGPEADMTTALDVIEERCRMAFEGVPNETRKSFEDGTTIFERV
                     LPGADRMYPDTDSAPIPLEDELIEELRKNQPADVIDRYHQLVAWKVPEDTYTYIFSKN
                     LFPVIEKIIKDLKINPRLVGTFFGHHLKWVEGQHVKSAEFSYEMIFALFRYLKENKLD
                     LILAGNMLPVIYLHPKMDFESVLTSIGFKKVKKEDILAKAEFLNEKFKQIGKKQSPIT
                     RHNWVMGELRKVALGNIDLTELSKEIKHFV"
     gene            1314..2699
                     /gene="gatD"
                     /locus_tag="PKZ74_08065"
     CDS             1314..2699
                     /gene="gatD"
                     /locus_tag="PKZ74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPM92753.1"
                     /translation="MTDDIFQGYKGDALATLKKFNTRVWGQTVVETTRGTFTGTVLPR
                     AENDDDKHIVVKIPTGYNIGVDVRTITSMKETGYKKAVYKIPEKEFPITPGLPKVKLL
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLETEKIFAVFSENMGPKQYV
                     ALSKAIGKEIENGIDGIVIGHGTDTLHHTGAVLTFMCQNLPVPVVLVGSQRSSDRPSS
                     DAALNLMHAMTAAGTGDIAEVMVCMFGPTSDEYGLLHKGTRVRKMHSSYRSTFRTIGD
                     VPVARVSRKGVIPIHKNYNKRRNDRNVNIIPYFSDEVTMLYYYPGIKPDVMDAMIDAG
                     YRGIIIVGTGLGHVNKELYPALVRAKEKGVHVFMTVQTIWGYVHMFVYDTGRDMMAKG
                     VVPAGNMLPEVAWVKLSWILGQTNDPQKVRQMMMTPVNDEITLREPYNGYLVYQGGVP
                     EVEEFVRKVHK"
     gene            2894..4951
                     /locus_tag="PKZ74_08070"
     CDS             2894..4951
                     /locus_tag="PKZ74_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92754.1"
                     /translation="MNRSLLIISLFFWALLIQAQKTTVHDEYIVDRVIDENGKELVQI
                     IVPGKPPDHFRMPAVIPSNAVTAYSLGNVPAYDWSFGCSATSAAMMAGYYDRTSYPNM
                     YTGPTNGGVAPMDNSVWGSVVINGETRKQCPFSATRNGLDGRTIRGHVDDYWIQYGNT
                     GTDPFITNGWTEHTYGECTGDYMKTNQSNYGNSDGSTTFWYYNDGSPYAGNYNNNDGM
                     YGIKLFFESRGYTVTSYYNQYIYGYLGNTLGFTFNQYMAEIDAGRPVLIQVDGHTMLG
                     MGYNETGSIVYLHDTWDYSTHQMTWGGSYSGMAHYGVGVIQLQVITNDPCSNISVIGG
                     CGSGYSQTYTGGGSGQWYTTTANPCGYLTPGIEKIYSFVAPYNGTYSIQVTAASGYVD
                     YLWKSGSCASTGWNCIDDITSPGAYGAMTWSAGTTYYILLDDENSTTGTHTFYINFPI
                     CYDCPSYNLNLAPSTSWQTHSSSHVSYGCKTYRISVTSGLPYIFKTGCGNGATASYNT
                     LLELYNSGCTLVASNDNGCESNRSSISWTANYTGYAYLKVKGVGTASGSYTLAYINNS
                     VPANRLVQNVTVLSGQSPCYDATQNVITAGSGTTFIVQSGGDVSLIAGSSVLMLPGTL
                     IQSGAYLLGSITTNGSYCYTQAAPGQEQPVIRTIELIPGVMAIPVVGVESEEREQGTG
                     NRE"
     gene            4994..5707
                     /locus_tag="PKZ74_08075"
     CDS             4994..5707
                     /locus_tag="PKZ74_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92755.1"
                     /translation="MKKNVILVLVLFMMAGLANAQWAVNGSHIYNTNAGNVGIGMNAP
                     TSLLDVAKSMTEPTISVRNLGSNGGATYRMYDQLSGADWKFKATTTGGFKIRDNAFSL
                     DVMIVEANSAANVIYINAAGNVGIGTAAPSEKLAVNGNIKCKQVEVSLAGWSDFVFED
                     DYDLMPLAELEAYINQHNHLPDVPSAEEVISNGNNLGEMDAILLQKIEELTLYVIELK
                     KENEALKQAIGIPAEKIVH"
     gene            5858..7444
                     /locus_tag="PKZ74_08080"
     CDS             5858..7444
                     /locus_tag="PKZ74_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPM92756.1"
                     /translation="MKRITPLIILMLMAALLIAQQSMPEGKYIADRYIDENGREVVII
                     IVPGTPPGDHREPAAIPDRASVILTNVPAYDWSFGCSATSAAMMSGYYDRTSLPNCYT
                     GPTNGGVAPLDNSVWGTVVIGGETRSQCPFSATRQGLDGRASRGHVDDYWIQSDNPGP
                     DPFIVNGWTEHTYGDCTGDFMKTNQSTYGNVDGGTVFYNYVDGSPFSDPGGGNNDGMY
                     GLKLFYESRGYYVASHFNQYIMGYNGNGTGFTFADYKAEIDAGRPVLIQVEGHTMLGM
                     GYNDTGNTIYLHDTWDYTMHSMTWGGTYAGMLHYGVGVIRVYANPVGPCASVIPINGT
                     GAGNAKTYTGGGTGEWFQTGSNPCGYTSPGIEQVYSFTAPFTGTFNIQVTAANGWVDY
                     FWRAESCNPTGWTCISDIINPGAYGNLSWNSGTTYYILLDDEDNTAGTHTFYINGVQT
                     VPDNLTLQNITIASGQTECYGAAQTITTAGGGTTFTVQSGGTATLIAGQNILMLPGTL
                     LQSGSSVLGKITTNGTYCIP"
     gene            7508..8755
                     /locus_tag="PKZ74_08085"
     CDS             7508..8755
                     /locus_tag="PKZ74_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92757.1"
                     /translation="MVLFSGRKCLIVLGMHRSGTSAITGALSETGFEIGKNIMPPADE
                     NPKGFFENSSIVSLNDQILSELYVFWDDTLFIPDGWWKSEIFSGYKEKIRIILEDEYP
                     GDKPMLVKDPRMCVLLPLYLEVLQQMGTEPAFLVCVRHPLPVARSLGRRNNMPAEKAS
                     LLWADYQFKAELYSRGFSRLFVAYDEFLSDPLQVLYSIKDKLVPEMNIPDEVAGRIGS
                     FVDPALSNRFPESNRLYDFPYTGIANLYTIQQSSHLRDLKEDERVTTDGIRHQFENLN
                     RFFNGMPDQYRAALTVEFENGETIVLIAPIVFGTNTLEYPVSVQKKVKKLIFRPCNAK
                     TGIRWIEAEAYGPEENKIILAFTGTNATAKNAEKQVYIFETGLSKILFDFPEPFFASR
                     IIISIRYLVFGPAPCRKALWRKD"
     gene            complement(8752..>9098)
                     /locus_tag="PKZ74_08090"
     CDS             complement(8752..>9098)
                     /locus_tag="PKZ74_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM92758.1"
                     /translation="WGPLFGVPLPVHENETPSASFRYWVEAHKRILALQKEIGRDRIL
                     ILNFDRLCEDPERGIREILEFLGIQPSEELLARAAKLPVTPKTAGRWRQHPEDVFDEN
                     DLEFLRKMGFDG"
BASE COUNT         2372 a         2334 c         2312 g         2080 t
ORIGIN      
        1 gtttcctgaa ccggagcacc ggcaaagtgc gcaccgggat gggagccggc cgcgaagacg
       61 tcaatgtgag ctgccgcggc ggttcccggg tagagatcaa aggcgtggca catacccgct
      121 ggataccgga actgacgcac aacgaagcat tccgccagtg ggcgttgctc cacatccgcg
      181 aaacgttgaa acagagggtg aaaaatccgg gcaactggaa gatcgcttcc aaaatcctgg
      241 cctatcacaa tttcaatttc aagtataagc cgctacgaga ggcccgcaac agcaaaaaca
      301 ggatcgtggc tgttaacctg cccgatttca aaggcctgat gtcgcatttc acccagcccg
      361 gcaaaatatt tgccgacgag atcagcgacc ggctcaaggt gatcgcctgc atcgaaaaac
      421 cccatatggc tcattccgaa gatcttacgc cactggtcac tttgaaagac tgggaaacga
      481 tccggaaaat gttcaaggca aaggaaaacg atgcccagtt gatcttctgg gggcctgaag
      541 ccgatatgac gacagccctc gatgtgatcg aagagcgctg caggatggcc tttgaaggag
      601 tcccgaacga aacgcgcaaa tcgttcgaag atggcacaac catcttcgaa agagtactac
      661 ccggcgccga ccggatgtac cccgacaccg actcggcacc gatcccgctg gaggatgaac
      721 tgatcgagga actgcggaaa aaccagcctg ccgatgtcat cgaccggtat caccagctgg
      781 tggcctggaa agtgcctgaa gatacataca cctacatttt ttccaaaaac ctgtttccgg
      841 tcatcgaaaa gatcatcaaa gacctgaaga tcaatccgag gctcgtcggg actttcttcg
      901 ggcatcacct gaaatgggtc gaagggcagc atgtgaaatc ggccgaattc agctatgaaa
      961 tgatctttgc ccttttccgt tacctcaaag aaaacaaact ggacctgatc ctggcgggaa
     1021 acatgctgcc ggtgatctac cttcatccga agatggattt cgagtcggtg ctgaccagta
     1081 tcgggtttaa aaaggtcaaa aaagaagata tcctggccaa agccgaattc ctgaacgaga
     1141 aattcaaaca gatcgggaag aaacaatcgc ctattacacg ccacaactgg gtgatgggtg
     1201 aactaaggaa agtggcattg gggaatatcg atctgacgga gttaagtaaa gagattaaac
     1261 attttgttta acgcattttg ttaaatgata atattttctt acagaataaa tatatgactg
     1321 acgatatctt ccagggctat aagggcgatg cgctcgccac actgaaaaaa ttcaacaccc
     1381 gggtctgggg gcagacggtg gtggagacca cccgcgggac ctttacaggc actgtattgc
     1441 cgcgggctga gaatgacgac gacaaacata tcgtggtaaa gattccgacc ggttacaaca
     1501 tcggcgtcga tgtgcggacg atcacctcca tgaaggagac tggctataaa aaagctgttt
     1561 acaagatacc tgagaaagaa tttccgataa ctcctggatt accgaaggta aaattgctcg
     1621 gaaccggcgg aacgattgct tcgcgtctgg attatcgcac cggcgccgta atccctgctt
     1681 tttcgccggg tgagctctac ggagccgtgc ccgaactggc cgacatctgc aacctggaga
     1741 cggagaaaat atttgccgtt ttcagcgaaa acatgggccc gaagcagtat gtagcccttt
     1801 caaaggctat cgggaaagag attgagaatg ggatcgacgg gatcgtgatc gggcatggga
     1861 ccgatacgct gcatcatacc ggcgcagtgc tcaccttcat gtgccagaac ctgcctgtcc
     1921 ctgtcgtcct ggtcggctca caacgttcga gcgaccggcc cagcagtgac gccgcgctca
     1981 acctgatgca tgccatgacg gccgccggca caggcgatat cgccgaagtg atggtctgca
     2041 tgttcggccc gacatcggat gagtacgggt tgttgcacaa aggcacccgg gtgagaaaaa
     2101 tgcattcttc ctaccgttct accttccgga cgatcggcga tgtacccgta gcccgtgtca
     2161 gccgcaaagg cgttatcccc atccacaaaa attacaacaa acggcgcaac gaccgcaatg
     2221 tgaatattat tccctacttc agcgatgagg taactatgct ctattactat ccgggtatta
     2281 aacccgatgt gatggatgcc atgatcgacg cggggtacag ggggatcatc atcgtaggta
     2341 ccggcctcgg gcatgttaac aaagagctct acccggcact ggtcagagcg aaagaaaaag
     2401 gggtccatgt cttcatgact gtccagacca tctggggcta cgtgcatatg ttcgtttacg
     2461 ataccgggcg cgacatgatg gccaagggcg tcgtcccggc cggcaatatg ctgcccgaag
     2521 tggcctgggt gaaactcagc tggatcctcg gccagaccaa cgacccgcaa aaagtccgcc
     2581 agatgatgat gacgccggtc aacgacgaga tcaccctccg ggaaccctac aacggttacc
     2641 tcgtttacca gggcggtgta cccgaagtag aggagttcgt taggaaggtg cataaatagt
     2701 tccaggttcc aggttccagg ttccaggttc cgggttccag gttccgggtt ccaggttcct
     2761 gattttcctt ttttaaattc attccaaata agcggaccga gtgcgatttc gctgcctgga
     2821 ttgccgatga taactatatt ttcctgtaaa tttacagtca tcgaaataat tcactccatt
     2881 aaccatgcct tctatgaaca gatctttgct gattatttca ttgtttttct gggccttact
     2941 gattcaggca cagaaaacta cagtacatga cgagtacatt gtcgaccgtg ttattgatga
     3001 aaacgggaag gagttagtac aaatcattgt cccgggaaaa ccgcccgacc atttccggat
     3061 gccggcggta atcccctcca atgcagttac ggcttattcg ctgggaaatg tcccggccta
     3121 cgactggagt ttcggttgtt ccgccacctc ggcagctatg atggccgggt actatgaccg
     3181 cacaagttat cccaacatgt ataccggtcc gaccaatggc ggtgtggctc cgatggacaa
     3241 cagcgtatgg ggcagtgtgg tcatcaacgg cgaaacccgg aaacaatgcc ctttcagcgc
     3301 tacccgcaac gggctcgacg gcagaacgat ccgtggtcat gtcgatgatt actggatcca
     3361 atatggaaat acaggaaccg atcccttcat caccaatgga tggaccgagc acacctacgg
     3421 ggaatgcacc ggcgattaca tgaaaacgaa ccaatccaat tatggaaatt cggatggatc
     3481 gacaactttc tggtattata atgatggttc gccatatgca ggcaattaca acaacaacga
     3541 cgggatgtac gggatcaagt tgttttttga atcacgaggc tatacggtga catcctatta
     3601 caaccagtat atttatggct atctgggcaa cacactgggc ttcaccttta accagtatat
     3661 ggcggaaatt gatgccggac ggccggttct gatccaggtc gacggtcaca caatgctggg
     3721 tatgggctac aatgagacgg gcagcatagt ttacctccat gacacatggg attattccac
     3781 ccaccagatg acctggggtg gttcttactc cgggatggcc cattatggtg taggcgtgat
     3841 ccagttgcag gttatcacca acgacccctg ctccaatatt tccgttatcg gaggatgcgg
     3901 ttcaggatat tcccagactt ataccggggg cggcagcggc cagtggtata ccactaccgc
     3961 caatccttgc ggctacctga ctccggggat tgaaaagata tacagttttg ttgcgccata
     4021 caacggcacc tatagtatcc aggttacggc cgccagcggt tatgtcgact acctgtggaa
     4081 aagcggctcc tgtgcctcaa caggatggaa ctgtatcgat gatatcacct ctccgggagc
     4141 ctatggcgcc atgacatggt cggccggaac cacttactat attttgctcg atgacgaaaa
     4201 cagcaccacc gggactcata ccttttatat caactttccg atctgctacg attgtccttc
     4261 ttataacctg aatttagctc catccacttc gtggcagacc cattcttcct ctcatgtttc
     4321 atacggatgc aagacctaca ggatcagtgt aacctcggga ttgccctata tctttaaaac
     4381 aggatgtggt aatggggcaa ccgccagtta caatacgtta ctggaattgt ataattccgg
     4441 atgtacgctg gtggcttcca atgataacgg atgcgaatcc aaccggtcat ccatctcatg
     4501 gacagccaac tacaccggat atgcctatct gaaagttaaa ggtgtaggga cagcttcggg
     4561 ttcatataca ctggcttata tcaataattc cgtaccggca aaccggctcg tacagaatgt
     4621 aacggtgctt tcaggccagt cgccttgcta tgatgccacg caaaatgtca tcactgccgg
     4681 cagcggcaca acatttatcg ttcagagtgg cggcgatgtc agcctgatcg caggtagttc
     4741 cgtcctgatg cttccgggca cgctcattca gtcgggcgca tatcttctgg gtagtatcac
     4801 caccaatggt tcctattgtt acacgcaggc cgcccccggt caggaacagc ctgtcatccg
     4861 cacaatagaa ctaattcccg gtgtgatggc gattccggtg gtcggtgtgg aaagcgaaga
     4921 aagggaacag ggaactggga atagagaata gtgaatagta gaatctgata ataaaaaata
     4981 aatattctaa accatgaaaa aaaatgtaat tcttgtgtta gtcctgttca tgatggcagg
     5041 gcttgccaat gcccagtggg cggttaacgg atctcatatt tacaacacca atgccggcaa
     5101 tgtggggatc gggatgaacg ctcccacctc gctgctggat gtggcaaaaa gcatgacgga
     5161 accaacgatc tctgtccgca acctgggaag caacggaggt gcgacttacc ggatgtacga
     5221 ccagctcagc ggggccgact ggaaattcaa agccacaacc actggcggat tcaagatcag
     5281 ggataatgca ttcagtcttg atgttatgat tgtcgaagcc aacagtgctg ctaatgtgat
     5341 ctacatcaat gctgccggca atgtcgggat cggaacggct gcaccatcgg aaaaattagc
     5401 agtgaacggc aatatcaaat gcaagcaggt ggaggtttcc cttgcgggat ggagcgattt
     5461 cgtatttgaa gatgactatg acctgatgcc gctggccgag ctggaagcct atatcaacca
     5521 gcacaaccac ctgccggatg tgccatctgc cgaagaggtc atcagcaatg gcaacaacct
     5581 gggcgaaatg gatgccatcc tcctgcaaaa gatcgaagaa ctcaccctct atgtgatcga
     5641 gctgaaaaag gagaatgaag cgctgaaaca agctatcggc ataccggcag aaaaaatcgt
     5701 ccattgagtc tttttttagc cacaaaggcg cactaaggat gtcacaaagg cgcacaaagt
     5761 gattaaatat ctgatcaata cctttgtgtt actttgtgaa gccctttgtg ttctttgtgg
     5821 ttaaccccta ttaatacaac ttaataggat cttcgcaatg aaaagaatta cccctcttat
     5881 tattttgatg ctgatggcag ccctgttgat tgcccagcaa tccatgcccg aagggaaata
     5941 tatcgccgac cggtatattg atgaaaacgg ccgggaagtc gtcataatca tcgtcccggg
     6001 aacgcccccc ggcgaccacc gcgaacctgc agcaattccc gaccgggcca gcgtcatcct
     6061 taccaatgtc ccggcttatg actggagctt cggttgttcg gccacttcag cagcaatgat
     6121 gtcgggatat tacgaccgta ccagcctgcc caactgctat accggcccga cgaacggagg
     6181 cgtggcaccg ctcgacaatt ccgtgtgggg aaccgtcgtc atcggcggcg aaacacggag
     6241 ccagtgcccc ttcagcgcta cccggcaagg gctcgacgga agggcatccc gcggccatgt
     6301 cgacgactac tggatccaat cggataaccc cggccccgac cccttcatcg tcaatggatg
     6361 gaccgaacat acttacggcg attgcaccgg cgactttatg aaaacaaatc agtcgactta
     6421 cgggaatgtg gatggcggca cggttttcta caattatgtg gatggttccc ccttctctga
     6481 tcccggtggc ggaaataatg acgggatgta cgggctgaaa cttttctatg aatccagagg
     6541 ttattatgtc gccagccatt tcaaccagta tattatggga tataatggca atggtaccgg
     6601 gttcactttt gccgattaca aagccgaaat tgatgccgga aggcccgtgc tgatccaggt
     6661 cgaaggccat acgatgctcg gcatgggata caacgatacg ggaaatacca tctacctgca
     6721 cgatacctgg gattacacca tgcactcgat gacctgggga ggcacctatg ccggcatgct
     6781 gcactatggc gtcggggtga tcagggtata tgcaaatccg gtcgggccct gtgccagtgt
     6841 tattccgatc aatggcaccg gtgccggaaa tgctaaaact tacaccggcg gcggaaccgg
     6901 cgaatggttt cagaccggtt ccaatccctg cggttacact tcgcccggga tagaacaggt
     6961 ttacagcttt accgccccat ttaccggcac ttttaatatc caggtaacgg cagccaacgg
     7021 atgggtcgat tatttctggc gggcggaatc atgcaaccca acgggctgga cctgcatctc
     7081 cgacattatc aaccccggtg cttacggaaa tctttcgtgg aacagcggaa caacctacta
     7141 catactgctg gatgatgaag acaataccgc cggaacgcat accttttata taaacggagt
     7201 gcaaacggtt cccgacaacc ttaccttgca gaacatcacc attgccagcg gccagaccga
     7261 atgctacggc gccgcgcaga cgattaccac tgccggcggc ggcactacct tcacggttca
     7321 gagcggaggc accgccacac tcatcgccgg gcagaatatc ctgatgctgc cgggaacgct
     7381 attgcagtcg ggcagcagcg tgctgggaaa aatcacgacc aacggaacgt attgcatacc
     7441 ataattctgc gatccataag gaaaattgac gatttcatcc tacatttgta aataatcact
     7501 tttccttatg gtcctttttt ccggaagaaa atgccttatc gtgctgggca tgcaccgcag
     7561 cggaacttcc gcaataaccg gagctttgtc agaaaccggg tttgagatag ggaaaaacat
     7621 catgcccccg gccgatgaaa atcccaaagg gtttttcgaa aatagcagta tcgtcagcct
     7681 gaacgaccag atcctgtcgg aattgtatgt tttctgggat gataccttgt ttatcccaga
     7741 tggctggtgg aaatcggaga tcttctccgg gtacaaggaa aaaatcagaa tcattcttga
     7801 ggatgaatat ccgggagaca agccaatgct ggttaaggat cccaggatgt gtgtcctttt
     7861 gcctctctac ctggaagttc tgcagcagat gggaacggag cccgcattcc tggtctgtgt
     7921 ccgccatcct ctgccggtgg cccggtcatt gggccggaga aacaatatgc ctgccgaaaa
     7981 agcttcgctc ctgtgggccg attaccagtt caaagccgag ctttattcca ggggattttc
     8041 gaggctgttc gttgcgtatg atgaattcct gtcggatccg ctgcaagtcc tctattcaat
     8101 caaagataag ctggttccgg agatgaatat cccggatgaa gtggccggca ggatcggttc
     8161 attcgtcgat ccggcgctct caaaccgctt tcccgaatcc aatcgcctgt acgactttcc
     8221 atacaccggt attgcaaacc tttatactat ccagcaaagc tctcatctgc gtgatctaaa
     8281 agaggatgag agggttacga ccgatggcat caggcatcag tttgaaaacc ttaaccgatt
     8341 tttcaatggg atgccggatc aataccgggc tgcccttacc gttgaatttg agaatggaga
     8401 aaccatcgta ttgattgcac ccattgtttt cggcactaat accctggaat acccggtaag
     8461 tgtacagaag aaggttaaga agctgatctt caggccgtgt aatgcaaaga ccggtatccg
     8521 gtggatagaa gcggaggcct atggtccgga ggagaataaa ataatactgg cttttacagg
     8581 aacgaatgca acggcaaaaa atgcagaaaa acaggtatat atttttgaaa caggcctttc
     8641 aaaaattttg tttgattttc ctgaaccgtt ctttgccagc cggatcatca tatcaatccg
     8701 gtacctggtc ttcggaccgg cgccctgccg caaggcactg tggagaaagg attagccgtc
     8761 aaaccccatt ttcctcaaaa actccaggtc attttcatca aaaacatcct ccggatgttg
     8821 cctccagcgt ccggctgttt tcggtgtgac cggaagtttt gccgcccggg ccagtaactc
     8881 ctccgaaggt tgaatgccga gaaattccag gatctccctg atccctcttt ccggatcttc
     8941 gcaaagcctg tcgaagttaa gaataaggat cctgtcgcgt ccgatctctt tctgcagggc
     9001 gagaatccgc ttatgcgcct ccacccagta gcggaaggag gcagatggag tctcattttc
     9061 atgaaccggt aacggcaccc cgaacagcgg cccccagt
//
LOCUS       DAOPNH010000129         9052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8440_length_9052_cov_5.942648, whole genome shotgun sequence.
ACCESSION   DAOPNH010000129 DAOPNH010000000
VERSION     DAOPNH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8440_length_9052_cov_5.942648"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKZ74_08095"
     CDS             complement(<1..622)
                     /locus_tag="PKZ74_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92759.1"
                     /translation="MSAPLAEQLRPKTLDDFLGQEHLVGPDAILRKVIESGNLPSMIF
                     WGPPGVGKTTLAFIIAKQLSKPFFILSAINSGVKDIRNVIDQATEGGGGAILFIDEIH
                     RFSKSQQDSLLGAVERGTITLIGATTENPSFEVISPLLSRCQVYILKSLDRKELVKLL
                     ETAARHLEGTIKKPIKIEEDEALLRISGGDARKLLNALELIVNTEIN"
     gene            complement(683..1372)
                     /locus_tag="PKZ74_08100"
     CDS             complement(683..1372)
                     /locus_tag="PKZ74_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM92760.1"
                     /translation="MPTILITGANGNLGLAVVSRLLDDGNRIIAVTGKSAAGILPHDK
                     NLVIKELDLMDEEKAENLVRSALKDDSGIDAAVLLAGGFAMGKLTDTGKADLDKMINL
                     NFYTAYNMVRPLLKHFLERGNGGQFILVGSRPGLDAATGKDFFAYSLSKALVFKLAEH
                     INAAGKEKGVTATVIVPSTIDTEANRKAMPGADFSKWVPAEKIADAISFSLSETGKMI
                     RESVVKIYNRS"
     gene            1713..2330
                     /locus_tag="PKZ74_08105"
     CDS             1713..2330
                     /locus_tag="PKZ74_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPM92761.1"
                     /translation="MSEIFTDITTWYMDHINYWTITLLMAIESSFIPFPSEIVVPPAA
                     YKAAQGELNLFLVIFFSTLGAMIGALFNYYLALTIGRAAIYSFARTRFARMMLVEPKS
                     IQKAEDYFVKNGKISTLIGRLIPAIRQLISIPAGLAKMPLKYFLLYTFIGATGWNIIL
                     ALLGYFAPKELFELYYKEISFVMIGLGVLFVMYLVYKGFFQKKSV"
     gene            2587..4389
                     /locus_tag="PKZ74_08110"
     CDS             2587..4389
                     /locus_tag="PKZ74_08110"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM92762.1"
                     /translation="MEKTIKLLGALLAFLAFTTFIACDKEEEEPDVIASFTFAVDQTD
                     FMKVQFTNQSTNFSALSWNFGDNSAVSAETNPVHIFPDLGDFVVTLTATSLNGKVTDV
                     FTATVTISDPNAELTKLVGAESKTWKLLRDGSTGRYPLLVFPYNVEDPFTPTTIWWAM
                     GLNNDELANRPCLLNDEWTFYRDGTLEFNNGGDTWAEGGVFKAPDNKCIDDTEAWIGV
                     NDEDISAWGSGTHAFELIAGTPQKLKAIGNGAHIGFFKSATEYEVMKLNPMVQDEVTY
                     DVVKLTDGTTDTLIVQCNYYFEVGDAAFGGSWRYTLVHYDDPNDEPPIPTNNPNPNFS
                     YVVDGLTVTLTNNSLYCDSYLWDFGDGGTSTEAAPTHTYAAEGIYVITLTGTNANGTA
                     SITSEVWISATVLTEEILKGGGTPWKVKVGEKTVFVGSGLGQSNWWAVPAADLQEGGG
                     WACMTDDEFTFLDGGVFTYDGKGVVRNDGYLAEGQGCIEEGTVTGNGAAFLSCTDHSY
                     VFTPATGTTRPIIDLTNGTDRAAFIGFYKGYYGGENGAAPGAEQPLPNGGLTTNKYEI
                     MGYVNDGTKEYILVSVDITADHNGTAAWSVILER"
     gene            4528..7449
                     /locus_tag="PKZ74_08115"
     CDS             4528..7449
                     /locus_tag="PKZ74_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92763.1"
                     /translation="MKKVYILLLFLALAAHAFPQGRTITGRVTDESGTPLPGVTIQLV
                     GTSTGTTTDLNGLYKIAVTTGTLRFSFVGFVAQEILLTNQTTIDIVLKEETSMLNEVV
                     VVGYGTQRKKDLTTAVSVVDDKEIRERPITSAAQALQGKAAGVQVTVPSGKPGVGLSV
                     RVRGSTSVIAGNEPLYVIDGVPTTNTNGLNPSDIASMSVLKDASSSAIYGARAANGVV
                     LITTKRGSDAAGPTVNFDAYAGFSKLRKTIEVLNTKQYRDLMEELKIAMDPSWTNFGN
                     WSDSVFSTGYHQSYQLSFAGGNDKTKYFFGGGYFSNAGMVEPAKFDRYTVRLNLDNQI
                     TNWLKFGLSMNVARLRTEDTPDNASSGRGGVFMSTLNTPPFMSVWAPDGSGQYYPNPF
                     QGSWENPVAYMFGADQEAIDNTMMGNMFLEATIIKNLTFKTRYGMDYMAHQWDYFLDP
                     VQTSWGRANDGLGQSNKTNSNTWLWENSFDYVKKFGDHNFTAMLGSSTQKYRSNGTWL
                     EGKGYRPGSSVKTINAANETTGSTWLDEWSLVSFFARATYDYKSKYYLTATVRQDGSS
                     KLAHHWGTMPSFSAGWRISSEKFMENANYIDDLKLRGGWGKNGNQEGIPNYARYGLTN
                     FTPSTETPYPSENPISYGNPDLKWETTTQTNIGVDLSMFNFRVNFTIDAYYKKTTDVL
                     LNVQLTNTLPVTTIMTNAGDIENKGIEFSINTININKKITWTTDLNMSFNRNKVLSLV
                     YTPVYYFGDIYSSGSKVSMVKPGQPLGVFYGLVAEGVDPATGDMLYKDVNGNGLLELG
                     ADRTVIGDPNPDFVFGFTNTLNYKKWDLNIFFQGSVGNDIYNATRVDLEGMFDSKNQS
                     VAVLDRWTPENTDTDVPRAVKNSTYNIANSSRFIEDGSYVRLKTVTLSYKIIDNDPKI
                     RIIKKFSVYLTGENLLTFTNYSGYDPEVSAYGSNAVSMGIDYGTYPQSINVILGLNVQ
                     F"
     gene            7461..8930
                     /locus_tag="PKZ74_08120"
     CDS             7461..8930
                     /locus_tag="PKZ74_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM92764.1"
                     /translation="MKKIIIILTTVFAVVSFTACNKFLDLEPKSNNIWVNDTGDSILY
                     QTANEVETALAGVYADFRNEYFELDYFIVGDAQSDDAYAGADNPWMFEVDDYNLNALN
                     GLVTRDWRYLYGTIGLANLVINNVMLCPDPSLTQERRNEILGEASFIRAYIYFRAVQT
                     WGEVPLQLKEVTTVSAEVLPEIYPLLFPDRAPVDSVYTQIISDLEVALANVKPVAEHK
                     GFATTGAVNAVLAKVYATMEPPDYNKVVQYCDAVINGGYSLLPNFEDLWNNSAENTAE
                     SIFEVNYEGTATNANWGVNMFLRLFGESDWKKFNVPSNDLVKAYDDEGDVIRRNSTII
                     FSEEGFPDNYWPQDNYPFIWKWRISGNNSPQNYIFLRLADIILLKAEALNELGDVNGA
                     AELVNQIRGRVDLPNTTAAGQDEMRLAIEKERRLELAFEGHRWFDLKRTGRAIEVINN
                     AVDQNGQKLGYNLTENRLLWPIPQGELDLNTSLVQNPGY"
BASE COUNT         2351 a         2245 c         2223 g         2233 t
ORIGIN      
        1 ggttgatttc tgtattgacg atcagttcca gcgcattcag cagttttcgc gcatccccgc
       61 ccgaaatgcg cagcagcgcc tcatcttcct caatttttat cggtttttta atagttccct
      121 ccaggtgcct ggcggctgtt tccagcaatt tgacaagttc tttccggtcg agggatttca
      181 ggatgtaaac ctggcatcgg gaaaggagag gggagatgac ttcaaaggaa ggattctcgg
      241 tcgtggcgcc gatcagggta atggtgcccc gctccaccgc gcccagcagc gaatcctgct
      301 gagatttgct gaagcggtgg atttcgtcga taaaaagaat agctccgccg ccgccctccg
      361 tcgcctggtc gattacgttc cggatatctt tcacccccga attgatcgcg ctgagaataa
      421 aaaacggttt gctgagttgc tttgctatga taaaggccag cgttgtcttc ccgactcccg
      481 gcggccccca gaagatcatc gaaggcaggt tgcccgattc gatcaccttg cgcagtatcg
      541 catccggccc gaccaggtgc tcctggccca ggaaatcatc cagggtcttg gggcgaagct
      601 gttctgctaa aggcgctgac attaatgttc aaagttcaaa gtgcaaagtt atcaatttcg
      661 gaagtgagtg gtgagatgta ttctatgacc ggttgtaaat tttaacgaca ctttcgcgga
      721 tcattttgcc ggtttctgac agagaaaatg agatggcatc tgcgatcttc tccgccggta
      781 cccacttcga aaaatcggcc cccggcatcg ctttccggtt agcctccgta tcgatagtag
      841 acgggacgat taccgtggcg gtcactcctt tttctttccc tgctgcattg atgtgttcgg
      901 ccagtttgaa taccaaagcc ttgctgaggg aataggcgaa aaagtctttc ccggtcgcgg
      961 catccagccc gggacgtgac cctaccagga tgaactgccc gccgttgccc cgttccagga
     1021 aatgcttcaa taagggcctg accatgttat aggcggtata aaaattcagg ttgatcattt
     1081 tatcaagatc cgctttgccg gtatcagtca gctttcccat ggcaaaaccg ccggccagga
     1141 gcacggctgc atctataccg gaatcatctt ttagagccga ccggacaagg ttttcagcct
     1201 tttcctcatc catcagatcc agttccttga tcaccaggtt tttatcgtga ggtaatattc
     1261 ctgctgctga ttttcctgtt acagcaatga tccggttccc gtcatccagc agcctgctga
     1321 caacagccag tcccaggttt ccgtttgcac cggtgattag tatggttggc atatctcttt
     1381 gtttttttta tctgaggaac aaatatatca gtaatctgtt caggattcgt gaatgtttca
     1441 aaatccggca ttcctttttc tttattcctc cgactactcc ggctttttgg tgctccgacg
     1501 actgaagccg tcggttattt ggttaggggt tttcgccgta ggcgataaac tactgaagct
     1561 ggagcaaaac gaaaaagaaa tatttccctg taggggataa acaaaaagcc agaagcaacg
     1621 gctttaatcg ttggagcacg aacctggtat tttcttccgg cgagcaaacc cgaaagatgc
     1681 tatttttgcc acgcaatcct aaaatgagaa ttatgtcaga aatcttcacc gatataacca
     1741 cctggtacat ggaccatatt aactactgga ccattaccct gctcatggct attgaaagct
     1801 cgtttatccc gttcccgtcg gagatcgtcg tcccgccggc tgcttataaa gcagcccagg
     1861 gtgagctaaa tctgttcctt gttattttct tttccaccct gggcgccatg atcggggcgc
     1921 tgttcaacta ttaccttgcg cttaccatcg gaagggcggc gatatactcg tttgccagga
     1981 cccgctttgc ccgcatgatg ctggtagagc cgaaatcgat acaaaaagcc gaagactatt
     2041 ttgttaaaaa cggaaaaata tccaccctga tcggccgtct gataccagcc atccggcagc
     2101 ttatctcgat cccggccggc ctggctaaaa tgcctcttaa atattttctt ttatacacat
     2161 tcatcggggc gaccggttgg aatatcatcc tggccctttt aggctatttc gcgccaaaag
     2221 agctgtttga actctattac aaagagatca gctttgttat gatcgggctg ggagtgcttt
     2281 ttgtgatgta cctggtttat aaaggattct ttcagaagaa aagcgtgtag ggataatttg
     2341 aaaatttgaa aatttgaaaa tgcgaaaatt tgaaaatgcg aaaatttgaa aatgtgaaaa
     2401 tgtgatatta tgaggtattt ataggtgtgg tcaaatattt tcaaaaaaaa tccttgaatt
     2461 gcattattga attatcattt cacataatat tgtattaatt ttggtataag ttagaaaaaa
     2521 ctttgtgatt tcgaccaaac ataatgaaat tcaatccact aataattaac actaaatttt
     2581 acaaaaatgg aaaaaacgat taaattgttg ggtgccttat tggcattcct ggcgtttacc
     2641 acatttatcg cttgcgataa ggaagaagaa gaaccggatg taattgccag ctttactttt
     2701 gcggttgacc agactgactt tatgaaagtg cagttcacca accagtcgac caacttctcg
     2761 gcattgtcat ggaacttcgg cgataactca gccgtttctg ccgaaaccaa cccggtacac
     2821 atattcccgg atctgggcga ctttgttgta actttaacgg caacctcgct gaatggtaag
     2881 gtaaccgatg ttttcaccgc tacagtcacc atcagcgacc ccaatgccga actgaccaaa
     2941 ctggtcggcg ccgagtcgaa gacctggaaa cttctgcgtg atggcagcac gggccgttac
     3001 ccgttgctcg ttttccccta caatgttgaa gatcctttta caccgaccac gatctggtgg
     3061 gctatgggcc tgaacaacga cgaactggcc aaccgcccct gcctcctgaa cgatgagtgg
     3121 accttctacc gcgacggtac gctggaattc aacaatggtg gcgatacctg ggctgaaggt
     3181 ggcgtattca aagcgcctga taacaaatgc atcgacgata ccgaagcctg gatcggtgtc
     3241 aatgatgaag atatctctgc ctggggcagt ggtacacacg ctttcgaact tatcgccgga
     3301 accccgcaga aactgaaagc aatcggcaac ggcgctcata tcgggttctt caaatcagct
     3361 accgaatatg aggtgatgaa actcaatcct atggttcagg acgaagtgac atacgatgtc
     3421 gttaaactga ccgatggcac aaccgacacc ctgatcgtgc agtgcaacta ctacttcgaa
     3481 gtaggagatg ccgctttcgg tggctcctgg agatataccc tggttcatta tgacgatccg
     3541 aacgatgagc ctccgatccc gaccaacaac cctaacccga acttcagcta tgtggtggat
     3601 ggcctgacag ttaccctgac caataactca ttgtactgcg attcgtattt atgggatttc
     3661 ggcgatggcg gaacttccac tgaagccgct ccgacccata cctacgctgc ggaaggtatt
     3721 tatgtgatca cgctgaccgg aaccaatgcc aatggaacag cttcgataac atccgaagtg
     3781 tggatttcgg caacggttct caccgaagaa attcttaaag gcggcggcac cccatggaaa
     3841 gtgaaagtcg gtgaaaagac tgtatttgtc ggtagtggcc tcggccagag caactggtgg
     3901 gcggtaccgg cggctgacct gcaggaaggc ggcggctggg cttgtatgac cgacgatgaa
     3961 tttacattct tagacggtgg tgtattcacg tatgacggta aaggcgttgt ccgtaacgac
     4021 ggatacctgg cagaaggtca gggatgtatt gaagaaggta cggttaccgg taatggagcc
     4081 gctttcctct cctgcaccga ccactcctat gtatttactc ccgctacagg aactacccgg
     4141 cctattattg acctcaccaa tggtaccgac agggctgctt tcatcggatt ctacaaggga
     4201 tactacggcg gtgaaaatgg cgctgccccg ggtgccgaac aacccctgcc gaatggtggc
     4261 ctgacgacca ataagtatga aatcatggga tatgtcaacg acggaacgaa agaatatatt
     4321 ctggtttctg tcgacatcac cgctgatcac aacggcaccg ctgcctggtc agtgatcctc
     4381 gaacgttaga gaatttattt ttggaattct tttcaggagc gggcagtaat gtcccgctcc
     4441 tgtttttttt atatttttat cttttaacaa ctcaatttta aacatcttga tattcagaat
     4501 tataagtaaa tatctaataa gttcagtatg aaaaaagtct acattttgtt attattcctg
     4561 gccctggcgg cacatgcatt tccccagggg aggactataa ccggaagagt aaccgatgaa
     4621 tcgggaactc cgcttccagg ggtgacaatc cagttggttg gcacctcaac cggtaccaca
     4681 accgatctca acggcttgta taaaatagct gtgacgaccg gaacgctgcg gttcagtttt
     4741 gtcggatttg tcgcgcagga aatactcctg acaaaccaaa ctacgatcga tatcgttttg
     4801 aaggaagaga cctccatgct taacgaagtg gttgtggtcg gctatggaac gcaacgtaaa
     4861 aaagacctga caacggccgt ttcggtggtc gatgataaag agatcaggga aaggccgatc
     4921 acttctgccg cccaggcgct tcagggaaaa gcggccggcg tgcaggtcac cgtcccgtcc
     4981 ggtaaaccgg gagtgggcct gtcggtcagg gttcgcggtt caacatccgt tatcgccgga
     5041 aatgagccat tatacgtgat cgatggtgtg ccgacaacca ataccaatgg gcttaacccg
     5101 tcggatatcg cctcgatgag cgtgctgaaa gacgcctctt catccgcaat atacggagcc
     5161 cgggcagcca atggagtcgt tctgatcaca accaaacggg gctccgatgc cgccggccct
     5221 accgtcaatt ttgacgccta tgccgggttc tcaaagctta gaaagacgat cgaagtgctg
     5281 aataccaaac aataccgcga cctgatggaa gaactgaaga ttgccatgga tccgtcgtgg
     5341 accaattttg ggaactggag cgattcggtg ttcagtacag gatatcacca gtcgtaccag
     5401 ctatcgtttg ccggcggcaa cgacaaaacc aaatatttct tcggtggcgg ctatttcagc
     5461 aatgcaggga tggttgaacc ggcaaaattc gaccgctata ccgtccgcct gaacctcgat
     5521 aaccagataa ccaactggct aaaattcggc ctcagtatga atgtagccag gctccgtacg
     5581 gaagataccc ccgataacgc cagcagcggg aggggaggcg tgttcatgag caccctgaat
     5641 acccctccgt ttatgagtgt gtgggccccc gacggcagcg gccagtacta cccgaacccg
     5701 ttccagggat catgggaaaa cccggtcgct tatatgttcg gcgccgacca ggaagccatc
     5761 gacaatacca tgatgggcaa tatgtttctc gaagccacca tcataaaaaa cctgacattt
     5821 aaaacaaggt acgggatgga ttatatggcc caccagtggg attatttcct cgacccggtg
     5881 cagaccagct ggggaagggc taacgacggc ctcggacaat ccaacaaaac caacagcaac
     5941 acctggctct gggaaaattc attcgactac gtgaaaaagt tcggtgacca taattttacg
     6001 gccatgctgg gcagcagcac ccaaaagtac cgctcgaacg gcacctggct ggaaggcaaa
     6061 ggctaccgcc ccggctccag cgtgaaaacg atcaatgcgg ccaacgaaac caccggctcc
     6121 acctggctcg atgaatggtc gctggtgagc ttcttcgccc gggctaccta cgattacaag
     6181 agtaaatact atctcaccgc aacggtcagg caggacggct cttcgaagct ggcccaccac
     6241 tggggaacca tgccctcgtt cagcgccgga tggcggatct cttccgaaaa attcatggaa
     6301 aatgccaatt atattgacga ccttaaactg cgcggcggct ggggtaagaa cggaaaccag
     6361 gaaggtatcc cgaattatgc ccggtacggg ttgacaaact tcacgccgag caccgagacg
     6421 ccttatcctt ccgaaaaccc gatctcatac ggaaatcctg atttgaaatg ggaaacgaca
     6481 acccagacca acattggtgt cgatctttcc atgttcaatt tccgtgtcaa ttttaccatt
     6541 gatgcctatt ataaaaagac gaccgatgtc cttctgaatg ttcaactcac caataccctg
     6601 ccggtcacca cgatcatgac caacgccggg gatatcgaga ataaagggat cgagttcagt
     6661 atcaatacca tcaacatcaa taaaaaaatc acctggacga ccgacctgaa catgtcgttc
     6721 aacagaaata aagtgctgtc gctggtttat acgccggtct actatttcgg ggatatttac
     6781 agcagcggct ccaaagtttc tatggtcaaa cccggccagc cgctgggcgt tttctatggc
     6841 ctcgtggccg aaggtgttga tcccgccacc ggcgatatgc tgtataagga tgtgaacggt
     6901 aacgggctgc tggaactggg cgccgaccgg accgtgatcg gtgatcccaa tcccgacttt
     6961 gttttcggat ttaccaatac cctcaactat aaaaagtggg atctgaatat cttcttccag
     7021 ggaagtgttg gcaatgacat ttataacgcc acccgtgtcg acctggaagg gatgttcgac
     7081 agtaagaacc agtcggtggc ggtcctcgac cggtggacgc cggaaaatac cgataccgat
     7141 gttccccgcg ccgttaagaa cagtacctac aacattgcca attccagccg ttttatcgaa
     7201 gacggctcct atgtcaggct gaaaacggtg actttatctt ataaaattat cgacaacgat
     7261 ccgaagatca gaatcataaa gaagttttcg gtttatttga ccggagaaaa cctgctgaca
     7321 ttcaccaatt acagcggata cgatccggag gtgagcgcat acggcagtaa tgccgtttcg
     7381 atgggtatcg attacggcac ctatccgcag tctattaatg tgatccttgg attaaatgtt
     7441 caattctaat aaatgctgcc atgaaaaaga tcattatcat actgaccacg gtttttgctg
     7501 tcgtatcttt cacggcatgt aataaattcc tcgacctcga accgaaatct aacaatatct
     7561 gggtcaatga cacaggcgat tcaattttgt accagaccgc caatgaagtg gaaacagcac
     7621 tggccggagt atatgccgat ttccggaatg agtatttcga actcgattat tttattgttg
     7681 gcgatgccca atcggatgac gcctatgccg gtgccgataa cccctggatg ttcgaggtcg
     7741 acgactataa cctgaatgcg ctcaatggcc tggttacccg cgactggcga tatctctacg
     7801 gaacaatcgg cctggctaac ctggtgataa acaatgtcat gctctgtcct gatccttctc
     7861 tgacacagga aagaagaaat gaaatcctgg gtgaagcttc atttatcagg gcttacattt
     7921 atttccgggc agttcagacc tggggagagg tgccgctgca attaaaagaa gttacgacgg
     7981 taagtgcaga ggtattgcct gagatttacc cactcctttt ccctgaccgc gccccggtcg
     8041 atagcgttta tacgcagatc atcagcgacc tggaagtggc cctggcgaat gttaagcctg
     8101 tagccgagca taaaggattt gcgacgacag gcgcagtgaa tgccgtcctg gccaaggttt
     8161 atgcaactat ggaaccgccg gattataata aggtagtgca atactgcgat gccgtcatta
     8221 atggtggcta cagcctttta cccaattttg aggacctctg gaataattcg gcggaaaata
     8281 cagccgaatc gatctttgag gtcaattatg aagggacagc cactaatgcc aactggggcg
     8341 tcaatatgtt cctgcgatta tttggagaat cggactggaa aaagttcaac gttccttcaa
     8401 acgatcttgt taaagcctat gatgatgagg gcgacgtgat ccggagaaac tccaccataa
     8461 ttttttcgga ggaaggtttc ccggataatt actggccgca ggataactac ccgttcatct
     8521 ggaaatggag gataagcgga aataacagcc cgcagaacta catctttctc cgtttggccg
     8581 atattatcct gctgaaggcg gaagcgctca acgaactggg tgatgtgaat ggcgcggctg
     8641 agctggtcaa ccagatccgc ggcagggtcg acctgcccaa taccactgct gccggacagg
     8701 atgaaatgcg cctggcgatt gaaaaagaac gccggctcga gctggctttc gaagggcacc
     8761 gctggttcga tctgaaacgg acaggccgcg ccatcgaagt catcaacaac gccgtggacc
     8821 agaacggtca gaagcttggc tacaacctga cggaaaaccg gctcctgtgg cccattcccc
     8881 agggtgaact cgatctgaat accagcctgg tacagaatcc gggttactga tatcaatgtt
     8941 gaattttgaa ttttgaaggt tgaatgaagg atgaaatttg ttatttggcc gttcttcttg
     9001 cgctgctgct cctgcccggc tgcaaggatg aaccggcgga gaaggaaggg cc
//
LOCUS       DAOPNH010000130         9046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8448_length_9046_cov_8.113892, whole genome shotgun sequence.
ACCESSION   DAOPNH010000130 DAOPNH010000000
VERSION     DAOPNH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8448_length_9046_cov_8.113892"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..1678
                     /locus_tag="PKZ74_08125"
     CDS             374..1678
                     /locus_tag="PKZ74_08125"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HPM92765.1"
                     /translation="MKKIGIRYEDKYVMERRVPLVPAHLKRLIQDEGLEFAVETSAKR
                     VFSDEEYEKAGAVVTDDLSGCPVIFGVKEIPIPRLEADKTYIFFSHVIKGQPHNMPML
                     KRLMELNCTLIDYERVVDEMGKRLIFFGRYAGLAGMINSLWSLGQRLEEFGVITPFLD
                     IAQTHNYHSLDEVRKVVSKVGQKIIEKGLPPELKPLVIGFTGYGNVSQGAQEIASLLP
                     VKEILPDELFNLHKHTHLPDNIIYKVVFKEEHLVKPKNPDHAFDLQDYYQHPENYCSQ
                     FEQYIPYLTVLVNCIYWDPRYPRLLTKEFTEQLFKDGKPKLTVIGDITCDPDGSVEIT
                     HRGTEIENPVFVYNPSTRKATDGFKGKGLLVMAVDILPSELPRDASRFFSEVLWRYVA
                     PIAKTDFSQPFEKVKLPGPIRKATILYKGELTPDYEYLQKYI"
     gene            1758..3095
                     /locus_tag="PKZ74_08130"
     CDS             1758..3095
                     /locus_tag="PKZ74_08130"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPM92766.1"
                     /translation="MKKVLILGAGMVVNPIVRYLLDKNFIVTVATRTKSKADEMIGGH
                     PNGRSVAWTVEQEAELDQMIAEHDLTVSLLPYLYHVMVAKKCIRLKKNMVTTSYVKPE
                     MKALDAEAKEAGIIILNELGLDPGIDHMSAMRIIDHIHGKGGNVDEFYSFCGALVAPE
                     VEENPFHYKFTWAPKGVVMAGNNDGNFLKDGVVKYIPTEQLFRNPLKVDFPKVGLLDV
                     YPNRDSLPYVELYGIPEAKTIMRGTFRYEGWCEIIDALKVLKLITYDKFNMEGMSYAG
                     MMAKMIGESSTENIVAKVAAKLGVKINARAIVAMEWLGLFSNEPMNRKEDSTFEVVSD
                     LMIAKMMIQPDERDMVVMQHTFLASYPDGKQEVIKSRMLDFGTLKTDTSVARTVALPA
                     ACGVEMILEGKITVKGVYIPVIPDIYNPILDQLETMNIKMVEEYGLSVSERIS"
     gene            3295..4017
                     /locus_tag="PKZ74_08135"
     CDS             3295..4017
                     /locus_tag="PKZ74_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPM92767.1"
                     /translation="MAKLRFAKWIGGGLGWAWGGPIGGILGFIFGAMIDGMQSGKFEY
                     KPTQSGDFSVSLLILSAAVMRADGKVMKSELEYVRQFFSRQFGQEVANQRIQMLQEIL
                     KQDFNLPEVCQQISHYMDYPAKLQLLHYLFGICAVDNQYNPKEVEMVGTIAGFLGIER
                     QDFESIRAMFVKDIDSAYKILEVNPDATDEEVKAAYRKMAVKYHPDKVEHLGPEIRKS
                     AEEKFQQLQAAYDEIKKRRGIK"
     gene            complement(4198..6120)
                     /locus_tag="PKZ74_08140"
     CDS             complement(4198..6120)
                     /locus_tag="PKZ74_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92768.1"
                     /translation="MAKIKIFQIFLLFTIHFSLFIIPCSLLADKLDSLMRVHDTSTVV
                     YFFHRTDSLAMGRFYTVESNRLTGFQRYNPLDQQGRFSASSSNTGLPYKNLIFRPLLS
                     GGFFTGMESYDAFTYSSENTRYYRHLIPVTFLQYSSGPEKEQLFRVVHSHTIKRLVTL
                     GVDFNLINSPGHFINQKSDDKSVVFTGQYYTKDLRFGAIANYRHNKFISGENGGIVND
                     TVFEEQLETDTKLFDVNLTTAQNLVKESGFMANTYFYLSGKPEIIDSTKMRPPTFHAG
                     RIDYTFNYTRAIQIYSDQDPLADFYQPFGPVIDSTETFDSLMINQYENRFSWSNLRIG
                     DVPEKKYLTIIFAAENKIATIADSVSDRRFSSWIPEASMIIRPYKTLLIRLNGRYTIG
                     DFNNTGFVLQGLANQHYRDKKGRSGDIIFSFLTTSQQAGYFYGHFNSNYFRWDTSFSN
                     QIMQLATFSLRYQGLTARVEYQFISDYVYLNQQALPSQYKGSINLIRAELNEEFRWKV
                     WAFNAQVIYTINSNKDLIRVPDLMARASFSPTFSLFKNAAVLQPGVEVFYNTAYYASA
                     YMPALRMFYLQDEKKIGNYAYLDVFINLMIKRFRIFVKYQHLNSLWSEHRYYMVPHYP
                     MQEGAFKFGLSWSFYD"
     gene            complement(6288..8153)
                     /locus_tag="PKZ74_08145"
     CDS             complement(6288..8153)
                     /locus_tag="PKZ74_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM92769.1"
                     /translation="MRTFLEVTNLSRVYGEKVLFKNISFTIAEGQKVALIARNGTGKT
                     SILNTIAGTESADEGSAVIVSGISYGYLSQDPVLNPAKTVFDEVYDSANNIQRTILAY
                     EQALESHNENALHQAMTEMDACNGWDYEIRIKQILSTFKINNFDQKISELSGGQRKRV
                     ALAKTLITEPEFLILDEPTNHLDVEMIEWLEEYFSRSSTTLLMVTHDRYFLDRVCNEI
                     IELETGSLYRYKGNYSYFLEHQAERVDIQNKEVEKARNILRTEQEWMNRMPKARTTKA
                     KARIDSFYEIKEKATQKFTDDRVRINIQGERLGNKILEVKDISFSWPDVPVVDHFGHI
                     FKKNEKVGIIGKNGTGKTTFLDLLSGKIRPQKGRVEPGETVKFGYYRQEGLSFKEDDR
                     VIDVVRRVADEVKTASGDWMSAAAFLNYFLFPYHTHNQFVYKLSGGEKRRLYLVTVLM
                     QHPNFLILDEPTNDLDILTLNVLEEYLVNFNGCVMVVTHDRFFLDKIVDHLFVFEGNA
                     KVKDYPGNYSQWKEWAEKQELKNKAIQKAKEPVTKRPQREKKPGLTFKEKRELEQLEK
                     DLELLEEEKYTIESSFNDSNLSPDHLIKKSERLGEIIKEIDVKSERWIELSDLAI"
     gene            complement(8536..8647)
                     /gene="rrf"
                     /locus_tag="PKZ74_08150"
     rRNA            complement(8536..8647)
                     /gene="rrf"
                     /locus_tag="PKZ74_08150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8735..>9046)
                     /locus_tag="PKZ74_08155"
     rRNA            complement(8735..>9046)
                     /locus_tag="PKZ74_08155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2396 a         2137 c         2114 g         2399 t
ORIGIN      
        1 cttatattta tttcgctctg tcgcatgcta cagccggtta ggctcctacg gagcttatat
       61 ttatttcgct ctgtcgcatg ctacagccgg ttaggctcct acggagctga tttgaatttc
      121 ccggatttgc agaatcgcaa ggctccgtca ggagccccac cgtctgtagc gccggacgtt
      181 tcacgggatt cccccggctc cgtcaggagc cccaccgtct gtagcgccgg atgatgcaat
      241 gttaataccc aagctccgtt aggagcgtcc cgtattaaaa tacccgatct attgatttgt
      301 gaaatattta acgataaccg aaaatctccc cgaaaattgc taagtttgcc cgcaaattgg
      361 ctaaaacgac tgcatgaaaa aaatcgggat tcgctacgaa gacaaatacg tgatggagcg
      421 ccgcgtaccg ctggttccgg ctcatcttaa aagactgata caggacgaag ggcttgaatt
      481 tgccgttgaa acatcggcta aaagggtttt ctccgacgaa gaatatgaaa aggcaggtgc
      541 agtagtgacc gacgacctga gtggttgccc ggtcattttc ggcgtaaagg agatccccat
      601 tccccgcctg gaagcggaca aaacgtatat tttcttttcc catgtgatca aaggccagcc
      661 gcacaatatg ccaatgctga aacgattgat ggagctgaat tgcacgttga tcgactatga
      721 aagggttgtg gatgaaatgg gcaaaaggct gatctttttc ggaagatatg ccggcctggc
      781 agggatgatc aattcgctct ggtcattagg gcaaagactt gaggaattcg gcgttattac
      841 accatttctc gatatcgcac aaacccataa ttatcattca ctggatgaag tgcggaaggt
      901 ggtatcaaaa gtaggtcaga agatcattga aaagggcctt cccccagagc tcaaaccact
      961 ggttatcggg ttcacgggtt acgggaacgt gtcgcagggc gcccaggaga tcgcttcgct
     1021 gctgcccgtt aaggagattc tgccggatga acttttcaac ctccataaac atacccacct
     1081 cccggataat atcatataca aagtagtttt caaggaagaa cacctggtca aaccgaaaaa
     1141 tccggatcat gcgttcgatc tgcaggacta ttaccagcac ccggaaaatt actgcagcca
     1201 gtttgagcaa tacatccctt acctgacggt cctggtgaat tgtatctact gggacccacg
     1261 gtatccaaga cttttaacta aagaatttac cgagcagctt tttaaagatg gaaagcccaa
     1321 gctgaccgtt atcggggata ttacatgcga tccggatggt tccgtcgaga tcacgcatag
     1381 aggaacggag atcgaaaacc cggtatttgt ttacaatccg tccactcgaa aagcaacgga
     1441 tggtttcaaa gggaaaggat tgctcgtcat ggcggtagat attctgccca gtgagctgcc
     1501 ccgcgatgcc tcacgttttt tcagcgaggt gctctggcgg tatgtggccc caattgccaa
     1561 aaccgatttc agccagcctt ttgagaaagt aaaactgccc ggcccgatcc gtaaagccac
     1621 catcctgtac aaaggcgaac tcaccccgga ttatgagtac ttgcagaagt atatctaaat
     1681 tggaaaatgg aaattggaaa tttggaacct gtaacagttt aacaatttaa caatcgaata
     1741 atagaacaat agaactcatg aaaaaagtcc ttatccttgg agccggcatg gtggtaaatc
     1801 ccatcgtgcg ttatttactc gacaaaaact ttatcgtaac cgtagctacc cgcactaaat
     1861 cgaaagccga cgaaatgatc ggcgggcatc ccaatggccg gtcggtggca tggaccgtcg
     1921 agcaggaagc cgaattggat cagatgatcg ccgaacacga cctgaccgtc agtctgctgc
     1981 cttatctcta ccatgtgatg gtggctaaaa aatgcatccg cctgaaaaag aacatggtga
     2041 ccacttctta tgtgaaaccg gaaatgaaag cccttgatgc tgaagcgaaa gaggcaggca
     2101 tcatcattct gaacgaactg ggtctcgatc cgggcatcga ccatatgtcg gccatgcgca
     2161 tcatcgacca tattcacgga aaaggtggta atgtggatga gttctattct ttctgcggcg
     2221 ctctcgttgc accggaagtg gaagaaaacc ctttccatta taaatttacc tgggctccca
     2281 aaggtgtggt catggccgga aataatgacg ggaatttttt gaaagatggc gtggtgaaat
     2341 atattcccac cgagcaactt ttcaggaacc cgctgaaagt cgatttcccg aaagtcgggt
     2401 tactcgatgt ttacccaaac cgcgattctc ttccttatgt tgaattatac ggcattcccg
     2461 aagcgaagac cattatgcgc ggcacgttcc gctatgaagg ctggtgcgag atcatcgacg
     2521 cgttgaaagt cctcaagctt ataacctacg ataaattcaa tatggaaggc atgtcatatg
     2581 ccgggatgat ggcgaaaatg atcggggaaa gctctacgga aaatattgtg gcgaaagtgg
     2641 ccgcaaaact tggcgtgaag atcaatgccc gcgctatcgt ggccatggag tggctcggac
     2701 tgttcagcaa tgagccgatg aaccgcaagg aagacagcac gttcgaagtt gtttccgacc
     2761 tgatgatcgc taaaatgatg atccagcccg atgaacgcga catggtggtg atgcagcata
     2821 ctttcctggc aagctatccc gatgggaagc aggaagtgat taaatcccgc atgctcgact
     2881 tcggtacttt gaagactgac acctccgttg cccggacagt tgcattgccc gctgcatgcg
     2941 gtgttgaaat gatcctggaa ggcaagatta ctgtgaaggg cgtttacatt cccgtgatcc
     3001 ccgacattta caacccgatc ctggatcagc tcgaaaccat gaatataaag atggtggagg
     3061 aatatggatt atcggtaagt gaacggatct cttaaattcc aggcactaaa ttccaggcac
     3121 caaataccaa gcaccaaatt ccaaacaagc accaaattaa aagttcaaaa accaatacta
     3181 ttttgaattt gattttttga atttgtttgg aatttggaat ttggaatttg gtgcttacag
     3241 ttaatggtcc aactgcttaa ctatttccta actttgccgc aataaaaaat agacatggca
     3301 aaattacgtt tcgccaaatg gatcggcggc ggcctgggct gggcctgggg cggccccatc
     3361 ggcggtatcc tcggcttcat cttcggagcc atgatcgatg gcatgcagtc gggtaaattc
     3421 gaatacaaac ccacacagtc gggcgacttc agcgtcagcc tgctgatcct ctcggcagcc
     3481 gttatgcgtg ccgatggcaa agtgatgaaa tcggagctgg aatatgtgcg ccagttcttt
     3541 tcccggcaat tcggacagga agtggccaat caacggattc agatgctcca ggaaatcctc
     3601 aagcaggatt ttaacctccc ggaagtttgc cagcagatca gccattacat ggattaccct
     3661 gcaaaactcc aacttctgca ttatctcttc gggatttgtg cagtggataa ccagtacaat
     3721 ccgaaagagg tcgaaatggt tggcactatt gcgggtttcc tggggattga aaggcaggac
     3781 tttgaatcca tccgcgccat gtttgtcaaa gatatcgaca gcgcttacaa aatcctcgag
     3841 gtcaatcccg atgcaaccga cgaggaggtc aaagcggctt atagaaagat ggcagttaaa
     3901 taccatcccg acaaggtcga acacctgggc cccgaaatcc gcaaatcagc cgaggagaaa
     3961 ttccagcagc tacaggcagc gtatgatgag atcaagaaac ggagagggat caaatagaaa
     4021 ttccaaattc caagcaccaa attccaaaca aattccaaat tccaagcacc aaatcccaaa
     4081 caagcacaaa atttaatatt caaaatccaa aacttttttg aatttgtttt ttaagttttc
     4141 gaatttgctt ggaatttggg atttggtgct tggaatttta tagttggtgc ttggaattta
     4201 atcatagaag ctccagctca gcccgaactt aaacgcccct tcctgcatcg ggtagtgcgg
     4261 aaccatatag tagcggtgtt cgctccataa actgtttaga tgctggtatt tgacaaagat
     4321 cctgaaccgc ttgatcatca ggttaataaa cacatccagg taggcatagt tacctatttt
     4381 cttctcatcc tgcaaataaa acatccttaa tgccggcata taagcgctgg cataataggc
     4441 cgtattgtaa aacacctcaa cacccggctg tagtaccgcg gcatttttaa aaagagagaa
     4501 agtgggactg aatgaagccc tggccatcag gtcaggcacc ctgatcaggt ctttattgga
     4561 attgatcgtg taaatgacct gcgcattgaa cgcccacact ttccaccgaa attcctcatt
     4621 taactcagcc ctgatcaggt tgatgctccc tttgtactgt gatggcagcg cctgctggtt
     4681 cagatataca taatcgctga tgaactggta ttcgaccctg gccgtcaaac cctggtatcg
     4741 cagagaaaac gtggccagtt gcatgatctg gtttgagaat gacgtatccc acctgaagta
     4801 atttgaattg aaatgcccgt aaaagtatcc tgcctgctgg cttgtcgtca gaaaactgaa
     4861 gatgatatct ccggatcttc cttttttatc ccggtaatgc tgattggcca atccctggag
     4921 cacaaatccg gtgttgttaa aatcgccgat ggtgtagcga ccgttgagcc tgataagcaa
     4981 tgtcttatac ggccggatga tcatcgaggc ttccggtatc cagctggaaa atctccggtc
     5041 ggaaacggag tcggcaatgg tggctatttt attttcggca gcaaatatga tcgtcaggta
     5101 ttttttctca ggcacatcgc caatccgcag atttgaccag gagaaccggt tttcatactg
     5161 attgatcatc agcgagtcga aagtctccgt cgaatcgatc accggcccga atggctggta
     5221 gaagtcggca agggggtcct ggtcgctgta aatctgaatg gcacgggtat aattgaaagt
     5281 gtaatcgatc cttccagcat ggaaagtcgg cggcctcatt tttgtcgaat cgatgatctc
     5341 cggcttgccc gacaggtaga aataggtgtt cgccataaaa cccgactctt tgacaagatt
     5401 ctgcgcagtc gttagattga catcgaaaag tttggtgtcg gtttccagtt gctcttcaaa
     5461 aaccgtatca ttgacaattc ctccgttttc cccggaaata aatttgttat gccgataatt
     5521 tgcgatcgcc ccgaatcgca ggtccttcgt ataatattgc cctgtaaaga caacgctttt
     5581 atcgtccgat ttctggttga taaagtggcc cggagagttg atcaggttaa aatccacacc
     5641 cagggttacc agccgtttta tggtgtggct atggactacc cggaacagct gctctttttc
     5701 cgggccgctg gagtactgca gaaaggtgac cgggatcagg tgcctgtagt accgcgtatt
     5761 ttcgcttgag tatgtgaacg catcgtatga ttccatcccg gtaaagaatc ctcccgaaag
     5821 gagaggtctg aatatcagat ttttataagg aaggcccgtg ttggatgaac tggcgctgaa
     5881 cctgccttgc tggtcgagcg ggttatagcg ctgaaaaccc gtcagacggt tgctttcaac
     5941 ggtataaaag cggcccatgg ccaggctgtc ggtacgatga aaaaaataaa caacagtaga
     6001 tgtgtcatga acccgcatca gcgagtcgag tttgtcggca agcagggaac agggaataat
     6061 gaatagtgaa aagtgaatag tgaataacag aaagatctga aatattttaa ttttagccat
     6121 tggatcgggt tgccgacttt tttaacggtt cgcgtgatgt tttatttttc aaaaatacgc
     6181 aaatcctatt tgatcaacca caaaggcgca caaagtaatg atttgtctat tgaccggcct
     6241 ttgtgtgcct tcgtgaaatc cttggtgtgc ctttgtcgtt attattttta aattgccaga
     6301 tcactcaact ctatccaacg ctccgattta acgtcaatct ctttaatgat ctctccaagc
     6361 cgttctgact ttttaatgag atgatccggt gaaaggttgc tgtcattgaa tgaggattca
     6421 atggtatatt tctcttcttc aagcaattcc agatccttct ccagttgctc cagctcccgt
     6481 ttctccttaa aggtcagtcc cggcttcttc tcccgttgcg gtctcttcgt taccggttct
     6541 tttgccttct ggatagcttt attctttaat tcctgctttt cagcccattc tttccattgc
     6601 gaataattcc ccggataatc tttaaccttc gcatttcctt caaaaacaaa gagatgatcg
     6661 acaattttat ccaggaaaaa acggtcgtgg gtgacaacca tcacacaccc gttaaaattg
     6721 acaagatatt cttccagaac attaagggta agaatatcca ggtcgttggt cggttcatcg
     6781 aggatcagga aattcggatg ctgcatcaaa acggttacca gatataatct tcgtttttct
     6841 cctccactaa gcttatacac aaactgatta tgagtatgat aggggaatag aaaataattg
     6901 aggaaggctg cggccgacat ccaatcgccg ctggccgtct tcacctcgtc ggcaacccgg
     6961 cgcaccacat cgatcacccg gtcatcttct ttgaacgata gcccttcctg ccggtagtag
     7021 ccgaatttga ccgtttcgcc cggttccacc cgccctttct gcggcctgat ctttcccgat
     7081 aagaggtcca ggaaagtggt cttgccggtt ccgtttttac cgattatacc gactttttcg
     7141 tttttcttga agatatggcc gaaatgatcg acaaccggaa catccggcca ggagaacgaa
     7201 atatctttca cttccaggat cttattgcct aacctttctc cctggatatt gatcctgacc
     7261 cggtcgtcgg taaatttttg cgtcgctttt tccttgatct catagaaact gtcgatgcgt
     7321 gcctttgctt ttgtggtcct tgccttcggc atgcggttca tccattcctg ttccgttcgc
     7381 agaatgttcc gggctttttc cacttccttg ttctggatat ctacgcgttc cgcctggtgc
     7441 tccaggaaat aggaataatt ccctttatat cgatataaag aacccgtttc cagttcgatg
     7501 atctcattac agactctgtc gaggaaataa cgatcgtggg tgaccatcag cagggtagtg
     7561 gaggagcggg aaaaatattc ttccaaccat tcgatcatct ccacatcgag gtgatttgtc
     7621 ggctcgtcga ggatcaggaa ttccggttcg gtgatcagcg ttttggccag ggcgacgcgt
     7681 tttctttgcc ccccggataa ttcgctgatc ttttgatcga agttattgat tttgaaggtc
     7741 gacaggatct gtttgatccg tatctcataa tcccagccgt tgcaggcgtc catctcggtc
     7801 atcgcctgat gaagggcatt ttcattgtgt gattccagag cctgttcata agccaggatt
     7861 gtccgctgga tattgttggc agagtcgtac acctcatcaa atacggtttt cgccgggtta
     7921 agcaccggat cctgggaaag atagccgtag gaaatgcctg aaacgatcac tgcgcttcct
     7981 tcatcggcgc tttcagtacc tgcgattgta ttgaggatac tcgtctttcc agtcccgttc
     8041 cgtgcgatca acgccacctt ttgtccttca gcgatggtga aggagatgtt tttaaagagt
     8101 actttttcgc cataaacccg gctgaggttc gtgacctcga ggaatgtccg cacgctaatt
     8161 gaattttggg caaagatata gacataagca ccaaatccca aacaccatcc cgagtactag
     8221 ggaccaaaca aattccaaat tcctaaactg ttttggattt gattttttaa gttttctcag
     8281 atgtttggaa attagagctt ggaatttgga atttccatcg taataggtgg ttaaaattct
     8341 attattatta aataaaaaac ccccggcttt tgaccgggtt tttgtataaa ttttggcgac
     8401 gacatactct cccatcccga gtcctcggga cagtaccatc tgcgctggcg ggcttatccg
     8461 ccaactggcg gatcggaatg ggaagaggtg agaaataaaa aaacccgtcc tgaaacagca
     8521 cgggttttaa ataaattttg gcgacgacat actctcccac aaataaatgc agtaccatct
     8581 gcgctggcgg gcttaacgac tctgttcgga atgggaagag gtggaccccg ccgcaatagt
     8641 caccaaaaaa ccttacgtcc gccggctggc ggactagttt tgtaatggca tgttgagaaa
     8701 gaaaatacag ttttggataa aatagcctga catttagcag aaagctacgg ataattagta
     8761 ctgctcgact ttgccattac tggctttaca tctgcagcct atcaacgtca tggtctatga
     8821 cgatccttaa aggaaacctc atcttgggct gagtttcgcg cttagatgct ttcagcgctt
     8881 atctcatccg aacatagcta ctctgcgatg cagctggcgc cacaacagat acactagagg
     8941 ttcgtccaac ccggtcctct cgtactaggg tcaggtgccc tcaagtttcc aacgcccaca
     9001 acagataggg accgaactgt ctcgcgacgt tctgaaccca gctcgc
//
LOCUS       DAOPNH010000131         8984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8534_length_8984_cov_5.987457, whole genome shotgun sequence.
ACCESSION   DAOPNH010000131 DAOPNH010000000
VERSION     DAOPNH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8534_length_8984_cov_5.987457"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKZ74_08160"
     CDS             complement(<1..362)
                     /locus_tag="PKZ74_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92770.1"
                     /translation="MTVSTTPGILRKWYYRILTGAVTGAGTWFLAEVIFNIKYRYYDL
                     VSMRISYVYAFIFGILILETYFRAGGFLDRILPWGKGPVKRFWAQLLAGFITAFWWII
                     VVRVGLNILLYPGQLIIL"
     gene            complement(456..806)
                     /locus_tag="PKZ74_08165"
     CDS             complement(456..806)
                     /locus_tag="PKZ74_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92771.1"
                     /translation="MKTAILTAAMMILMTAANARTIADTNYYQGSPVKTFQASVIKTG
                     PVMIRFSVLNPENDKVILKVYNEDQVKVYQKTVRKSNVTNIGCDMSNAEKGRYTFVVI
                     RNGKEEVKKSIILF"
     gene            1050..1592
                     /locus_tag="PKZ74_08170"
     CDS             1050..1592
                     /locus_tag="PKZ74_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92772.1"
                     /translation="MKTKTIQLIVMVLLVLGFTGCDKVSDLNTVTFDADFAASLDCST
                     AALKSTAENHAFSGTAVIDPASDTNVSDYLDNIKKYEVEAVHATITSMSQENVTVFTA
                     TLDVFNAGRETGWSMTNEVLTTGKTITLGNENGQWDTVSEILKDGQVFTVQINGEADT
                     GGVTFTLRVRIETKVEAKVI"
     gene            1730..2998
                     /locus_tag="PKZ74_08175"
     CDS             1730..2998
                     /locus_tag="PKZ74_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM92773.1"
                     /translation="MTRCRFLSVILLSLIFFSLHAQKHPISGVVLDSETYSPLINANI
                     SLTGSRTGCTSGLDGAFTIQVDTLPVYMIISYLGYETQHIWIDSKAEGLNVLMKPTAS
                     LLKEIEISSGKPEVFFKDDKYSILDYTIDHSFIYMIIFRYRTARAELICKKDNGEIIA
                     RSCTLPFKPTRLFADCLGYIHLLSPDSSYQVFLNKDTLLFPHRATLSKFYATLSDCIT
                     STADWLYFRKESNDKLSVSFYRINRHSGLRSEINTYRDEDAFRMLRDHPYDYYLLTMD
                     TLPASFNEMIAYSWVKKIQYKSNLSVMEKTGDSLVIINTTDGSVDILNISGYLVNHLQ
                     MPLSQHTGEKWMKNILNDPVSHCLYTTFLKNGRLHLYKLDLSKGHLQKTLTLAHSFPQ
                     HVMVHNNYLYYLYDVPGLMDNKQLLRESIR"
     gene            3120..3524
                     /locus_tag="PKZ74_08180"
     CDS             3120..3524
                     /locus_tag="PKZ74_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPM92774.1"
                     /translation="MKQLKSTGRELTKAEEQIMHIFWDKGSALVHDILKEFPEPKPAY
                     NTVSTIIRILEQKGFIGHKAYGRTYEYYPLISKNDYTRTYFRSFVANYFGNSYKSLAS
                     FFASEEKISIEELEEIRRLMDMEIQKQKAQKK"
     gene            3521..4807
                     /locus_tag="PKZ74_08185"
     CDS             3521..4807
                     /locus_tag="PKZ74_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPM92775.1"
                     /translation="MTDLLTYLLHSSAILAALVTVYWLFLRKETFFHVNRFYLVFSVL
                     LSLTMPFIRIGFFETETFRPAAILLEPVLITGEKVDKIAGSNISAFQLVAIVYFTGVV
                     IFTAKFIFQLIQLAVITRRFKIIRRDGSRFVLVDNGYSPFSFFNFIYIREEYLSDDKF
                     APVVAHEKVHIRQIHTIDIILAEFAVILQWFNPFAWLAGHAIKRTHEFLADEGVLKDG
                     FQKQKYQEFILQESIGIQLNTLTNNFNVSLLKNRIIMMTKKRSASWAKLKAGFALPSL
                     AVVLFLFTAGNPLWLTAQESQQKSEQAKAAQTVSQSAQEEPAFQVVKNMPSFKGGMDA
                     MTKYLVENIKYPPEAKTKGTAGTVFVTYVIEKDGSVTNVKILQGIGDGCDEEAARVVK
                     GMPKWNPGTNDNNEPVRVIFNLPIKFSLEGKKEEVK"
     gene            complement(4808..8602)
                     /locus_tag="PKZ74_08190"
     CDS             complement(4808..8602)
                     /locus_tag="PKZ74_08190"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM92776.1"
                     /translation="MILNFLKTTNPIQKFKKLLVGLSLVLLPTLLYPQGTQELNIYKM
                     INPDSPLPDSLYLIDIIKYIEDTLFLGGARISDIQYIGNPKGVGLFTDGADIGMNDGL
                     ILSNGWVGTTTIVDGGQNKTGAQAIPAHDSLGPYRAEPNGPPPLQDDDMDWLSGLFTP
                     NDPKPDTAYDPSIITFKFKPYYNSIKLEYVFASEEYLYEQPPFPPPPPPPIDVDMTGA
                     PVSDFMAVFIKRYPSEQNYNMIASLIGQDGDPSWIPVCVKTLNHTTPPGYYQPNYDRS
                     FIFDGASLPRQIFPFVSEGIDVTPCQNYWMKIAVADYPHGVVQGYDISHQINSAVFLK
                     AYSLMSGYGLEWTLEGAIDNPDFANDSSLVEGGCSNINITVKFNTMPKDTSFIRFRID
                     GASLADYTVTPPEAIVQDSLIMIPDSITEFTFTITAVDDNIAEGTNGIDHWFIRYQEN
                     PCDVPYQDTTGIGSNEDGYSGKIKVKVRDYNPFVNTSKSVGPDLPPPASQYHCGGDVT
                     VSIQDILEGGIPPYTYSWSNPPQIANTETFTTTIGDSPDMAICKIFDRCSGKPGYANG
                     SDTVFIYSRLEIEASGDFQLCQNGESDIEIVSTNVGDDYSTIWYFENPPGVLTQVGNE
                     SVYTVTWEDYGSFAPNTLTFICTATDECGNSANDTVHATFFPVVTISGVPLICLYDEI
                     QLTCSFALQYWWHYGSLAGPLLGQNQVLNYTPPNSGLHTIWVRIENECHEYADTSFTF
                     EVSELVMDMTMNNSTNFFTCPNVPFTLQELHAFDGWYWTWEDDGTNYEAYGQNISLEM
                     TEAGPHPVTVIAYNNHGCYDTITRTVTVYPYAYPVASTSIESVCIDYPTDLSIVPTGP
                     VTISSYFWTANPAYAWQPGQQTQASPTVTPQETTTFKCRITDNHGCLDSATVVVNVRP
                     RLAGNILGDPGFQCTDKPVTITFLPTVTPLPDATYSWTFDDGVPATSNLAVPPQIVWS
                     TDGLKNISLHIEELGCEETFYFQYQVYPDPLAGFTATGAAGCQPIQVSFTNISSNLEN
                     PSYLWDFGDGTTGTDVNPSHEYTIPGTYDITLTVTNSTGCVNTLTINDMVEVFEVPVA
                     DFTADPQAATIDNPTINFSEQVNIPYQIINWDFGDGNEDLSSPNPRHTYTLPGTYLVV
                     MYTETEHGCWDRDTLEIGIVEDIKIFVPNAFTPNGDGLNDCFAIGGTTGDIVDVFRII
                     IYNRWGVQLVDEPVHNPECLWNGRDVNGVEMPGGQYIFRIFGKNLRGSKKVYEGMVTL
                     LR"
BASE COUNT         2512 a         2033 c         2032 g         2407 t
ORIGIN      
        1 tccaggatga tcaattggcc ggggtagaga aggatattca accccaccct gaccacgatt
       61 atccaccaaa acgcagtgat aaagccagcc agaagctgtg cccagaacct tttaaccggc
      121 ccttttcccc aggggagaat tctgtcaaga aatcctcccg cacgaaaata ggtttctaaa
      181 attaggatcc cgaatataaa agcataaaca tagctgatcc gcatggatac caggtcatag
      241 taccggtatt tgatattgaa tatcacttcg gcaaggaacc aggtgcccgc tccggtcact
      301 gcacccgtca ggatacggta ataccatttt cgcaaaatac ccggagtcgt actgacagtc
      361 attttatgct ccctggatta attcttccaa accaatcaaa agtaggcaaa ttttcaacat
      421 gagaaagaac ggtataattc atcggaatgg cggtctcaga agagaataat gctcttcttt
      481 acctcttcct tgccgtttct tatgaccaca aaggtgtacc tgcctttttc ggcattcgac
      541 atatcgcatc cgatattggt aacattcgat ttgcggacag ttttctgata aacttttacc
      601 tggtcttcat tgtagacctt caggatcact ttgtcatttt cgggattcag aacgctgaat
      661 ctgatcatca caggaccggt ttttataact gatgcctgga atgtttttac cggagacccc
      721 tgatagtaat tggtgtcggc gatggtacgc gcattggcgg cggtcatcag gatcatcatg
      781 gcggcggtta agattgctgt tttcatggtt tctgtgtttt taaattgtta attgatgaaa
      841 caaagatatc ccgccatgaa ggggaaggaa atgaaaagat ggtgaactgc atgatcggaa
      901 gggtgaactg agtcgtctga caggtaaagt gaatttcgtt tcaggtgaat taaacttcaa
      961 ccatgggttt ttcgtctgat tttgcttctc ctggaataca atttctacat tttgaactta
     1021 actttgcata aatactaact attcatctta tgaaaacgaa aacgattcaa ttgattgtca
     1081 tggtattact agtgctgggt ttcaccggat gtgataaagt cagtgatctc aacacggtca
     1141 cttttgatgc cgatttcgcc gcatcattag attgctcaac ggctgcatta aaatccactg
     1201 ccgaaaatca tgcgttcagc ggaacagcgg taatcgatcc ggcatcggat acgaatgtca
     1261 gtgattatct ggacaatatt aagaaatatg aagttgaagc ggtccatgct accattacca
     1321 gtatgtcgca ggaaaatgtg accgttttta cagctacgct ggatgtcttc aacgccgggc
     1381 gtgaaaccgg atggtcgatg acaaacgaag tgctgacaac agggaaaacc atcacgctgg
     1441 gcaatgaaaa cggccagtgg gacacggtta gtgagatcct gaaagatggc caggttttca
     1501 ccgttcagat aaacggagaa gcagatacag ggggtgtaac atttactttg cgggtgagaa
     1561 ttgaaacaaa ggtggaagcc aaagtgatat agttctctgt gagactccat ctcgaaaaat
     1621 cgagatggag tctcactttt ccatccgtta acccaaacgc tcaaagggtt aaaacagtga
     1681 agtatacggt gtttaaattg tttagattta aacacaaaac tccaatgtaa tgacccgatg
     1741 cagatttttg tccgtcattc tactctcatt aattttcttt tctctccatg ctcagaagca
     1801 tcccatctca ggtgttgtgc ttgattctga gacctattcg ccgcttatta atgcaaatat
     1861 cagcctgacg ggcagccgga caggttgtac atcaggcctc gacggggcat tcaccataca
     1921 agtcgatacc ttgccggtct atatgattat cagctacctg ggatatgaaa cacagcatat
     1981 ctggatcgac agcaaagcgg aaggtttgaa tgtgctcatg aaacccacag ccagtctttt
     2041 gaaagagatt gagatatctt ccggaaaacc cgaagtattc tttaaagatg ataaatactc
     2101 tatcctcgat tacactatcg accactcatt catctatatg atcatcttcc ggtaccggac
     2161 agccagggca gaactgatct gtaaaaagga caacggggag atcattgcgc ggtcctgtac
     2221 cttgccattc aaaccgacac gattatttgc cgattgcctg gggtatatac acctgcttag
     2281 ccccgattca tcctaccagg tattcctgaa taaagacaca ctgcttttcc ctcaccgggc
     2341 cactctgtcg aaattctatg ccaccctttc cgattgtatc acgtcaaccg cagattggct
     2401 ctatttccgt aaagagagca acgacaaact ttcggtaagc ttttacagga tcaaccggca
     2461 ttcggggttg cgatccgaaa ttaacaccta tcgggatgaa gatgcattca ggatgcttcg
     2521 tgaccatccc tacgactatt accttttaac tatggataca ttgccagcca gtttcaatga
     2581 gatgatcgcg tatagctggg taaaaaagat ccagtataaa tctaatctgt cggtgatgga
     2641 aaaaacaggt gattcgctgg ttatcattaa tacaacggat ggctctgtcg atattctgaa
     2701 tatcagcgga taccttgtca atcatttgca aatgcccctt tcgcagcata caggtgagaa
     2761 atggatgaaa aacattttga atgatcctgt ctcgcattgc ttatatacta cttttcttaa
     2821 aaacgggagg ttgcatcttt acaaactgga cctctccaaa ggccatcttc aaaaaacatt
     2881 aacgctggcc cacagtttcc cgcaacatgt catggtccac aataactacc tgtattacct
     2941 gtatgatgtg ccggggttga tggataacaa gcaattgctc agggaaagta ttcggtaaga
     3001 ttaacgtgag actccatctc gaaaaatcga gatggagtct catttaaaaa aaatattact
     3061 tttttagttg ataaactaat tatttagtta tatatttgca gcatagaaat taaatgggta
     3121 tgaaacaact gaaaagcaca gggcgcgaac tcaccaaagc cgaggaacaa attatgcata
     3181 tattctggga taaaggatcg gcgctggttc atgatatcct gaaagaattt ccggaaccca
     3241 aaccggcata caataccgtt tccacgatca tccggatcct ggaacaaaaa gggtttatag
     3301 gccacaaagc gtatggccga acttatgaat attaccccct gatctcaaaa aatgattaca
     3361 cccggaccta tttccgcagt ttcgtagcca actatttcgg taactcctat aaatcactgg
     3421 ctagtttctt cgcgtctgaa gaaaagatca gcatcgagga actggaagaa atccgaaggc
     3481 tgatggacat ggagatccaa aaacaaaaag cacaaaaaaa atgacagacc tgcttacata
     3541 cctgttgcat tcatcggcta tccttgccgc actggtgacc gtttactggc tctttctcag
     3601 aaaagagacc ttcttccatg tcaaccggtt ctacctggtc ttttctgttc tgctttcact
     3661 gaccatgcca ttcatacgga ttggtttttt tgaaacggaa accttcaggc ctgccgccat
     3721 cctgcttgaa ccggtcctga ttacgggaga aaaagtcgac aaaattgccg gcagcaatat
     3781 ttcagccttt cagcttgttg caattgttta cttcacagga gtagtaatat tcactgcgaa
     3841 gttcattttt cagcttatcc agctggctgt catcaccagg cgatttaaaa ttatccggcg
     3901 cgatggatcc cgtttcgtcc tggtggataa tggctactcc cccttttcat tcttcaactt
     3961 tatttatatc cgggaagagt acttatctga tgacaaattc gctccggttg tggcgcatga
     4021 aaaagtacat atccggcaaa tacatacaat cgacatcatt ctggcggagt ttgcggtcat
     4081 tttacaatgg tttaatccat ttgcctggct ggccggccat gctatcaaaa gaacccatga
     4141 atttctggcg gatgaaggcg ttctgaagga tggttttcag aaacaaaaat accaggaatt
     4201 tattctgcag gaatctattg gcattcaatt aaatacctta acaaataact ttaatgtttc
     4261 attacttaaa aacagaatta ttatgatgac gaaaaaacgc tccgcatcat gggctaaact
     4321 aaaggccgga tttgcactgc cctcactggc tgtggttctt tttcttttca cagccggaaa
     4381 tcctttgtgg ttaactgctc aggaaagcca gcaaaagtca gaacaggcaa aagctgccca
     4441 gactgtttcc cagtcggccc aggaagaacc tgcattccag gttgtaaaaa acatgccttc
     4501 ttttaagggt ggaatggatg ccatgacaaa atacctcgtg gaaaacatta aatatccacc
     4561 tgaagcaaaa acaaaaggaa cagcaggaac cgttttcgtt acctacgtta ttgaaaaaga
     4621 tgggtcggtg accaatgtca agattctcca gggaattggc gacggatgtg atgaagaagc
     4681 tgcacgggtt gtcaaaggta tgcctaaatg gaacccgggc acaaatgaca ataatgagcc
     4741 agtcagggta atatttaacc tcccgataaa gttttccctg gaaggtaaaa aagaagaagt
     4801 aaaataatta ccttagcaag gtaaccatcc cttcatagac ttttttcgaa ccgcgcaggt
     4861 tcttcccgaa gatcctgaaa atatactgcc ctccgggcat ttcaaccccg ttcacatccc
     4921 tgccgttcca taaacattcc gggttgtgaa cgggttcatc cacaagctga acaccccaac
     4981 ggttataaat gatgatccgg aatacatcaa cgatgtcgcc cgttgtaccg ccaatggcaa
     5041 agcagtcgtt caatccatcg ccattcggtg taaaggcatt gggaacaaaa atcttaatat
     5101 cctccacaat accaatctcc agcgtatccc tgtcccagca tccgtgttcg gtctcggtat
     5161 acattacgac caggtaagtg cctggtaaag tgtatgtatg ccttggattt ggactggaaa
     5221 gatcctcatt gccgtcgcca aaatcccagt tgataatctg gtaggggata ttgacctgtt
     5281 ccgagaaatt gattgtagga ttatcaatag tagccgcctg aggatcagct gtaaaatcgg
     5341 ctaccggcac ctcaaacacc tcaaccatat cattaatggt caaagtattg acacaacctg
     5401 tcgaattggt caccgttaag gtaatatcat acgtccccgg tatggtgtat tcatgggaag
     5461 gattcacatc agtgcctgtt gtgccatcgc cgaagtccca caggtatgaa ggattttcaa
     5521 ggtttgatga gatattggtg aatgacacct gaataggctg gcaaccggct gcacctgttg
     5581 ccgtaaaacc ggccagagga tcgggataca cctggtactg aaaatagaaa gtctcttcgc
     5641 aacccagttc ttcaatgtgc aggctgatat ttttcaatcc gtctgtcgac cagacgatct
     5701 gcggtggaac ggccaggtta ctggtagccg gcactccatc atcaaatgtc caggaataag
     5761 tagcgtcggg tagtggtgtc acagtcggca ggaaagtgat cgtcaccggc ttgtcggtac
     5821 actggaaccc gggatcaccg aggatattgc ccgccagcct tggcctgaca ttgaccacca
     5881 ccgtggctga atcgagacag ccatggttat cggtgatcct gcacttaaat gttgtggttt
     5941 cctggggcgt aaccgttggc gaggcctggg tttgctgtcc cggctgccag gcatacgcag
     6001 ggtttgctgt ccagaaatag ctggaaatgg tgaccgggcc ggtcggtaca atgcttagat
     6061 cggtcggata atcaatacag accgattcga tcgaagtcga agccacagga taagcgtaag
     6121 gatagaccgt gaccgttcgg gtaatagtgt cataacaacc gtgattatta taagctataa
     6181 ctgttaccgg gtgaggcccg gcttcggtca tttccaggct gatattctgc ccgtaagcct
     6241 catagttcgt cccgtcgtcc tcccaggtcc agtaccaccc atcaaaagca tgaagttcct
     6301 gcagcgtaaa tgggacgttc gggcaggtga agaaattggt cgaattgttc atggtcatat
     6361 ccatcaccag ttccgagact tcaaaagtga aactggtgtc ggcatactca tggcactcat
     6421 tctcaattct tacccagata gtatggagtc ctgagttcgg aggggtataa ttcaatacct
     6481 ggttctgtcc gagcaaggga ccagccaggc ttccgtaatg ccaccaatac tgaagggcga
     6541 agctgcacgt cagctgtatc tcatcgtaca ggcagatcag cggcacacca ctgattgtga
     6601 caaccggaaa gaaggtagca tgcactgtat cgttagccga gtttccgcat tcatccgtcg
     6661 ctgtgcaaat aaaagtaagg gtattcggtg caaagctgcc atagtcctcc caggtaacgg
     6721 tgtaaacaga ttcattaccc acctgggtca atacacctgg aggattctcg aaataccaga
     6781 tggtggaata atcgtcgccg acattggtac tgacaatctc gatgtcggac tctccgttct
     6841 ggcaaagctg aaaatctccg ctggcttcaa tttcgagcct gctgtatata aaaaccgtat
     6901 cagaaccgtt cgcatacccg ggctttccgg agcatcggtc aaatattttg caaatggcca
     6961 tatcgggact gtcgccgata gttgtcgtaa atgtttcggt attggcaatc tgcgggggat
     7021 tcgaccatga atatgtgtag ggcgggatgc ccccttccag gatatcctga atgctgaccg
     7081 tcacatcccc gccgcaatga tactggctgg ctggaggagg aagatcggga ccgacagatt
     7141 tcgatgtatt gacaaacgga ttgtaatctc tgaccttgac tttaattttt cccgaataac
     7201 cgtcttcatt cgaaccgatg ccggtggtat cctggtaagg cacgtcgcag gggttctcct
     7261 ggtaacggat aaaccagtga tcgatgccat tggtcccttc ggcgatgttg tcatcgacag
     7321 cggtgatcgt aaaagtaaat tccgttatag aatccgggat catgatcagg gaatcctgga
     7381 cgatagcttc cggcggtgtt acggtataat cagccaaaga tgccccatcg atacggaacc
     7441 ggataaagga ggtgtccttt ggcatcgtat tgaatttcac tgtgatattg atgtttgagc
     7501 agccgccttc gaccaagctg gagtcgttcg caaaatcggg attgtcgata gcgccttcca
     7561 atgtccattc cagtccgtat ccgctcatca gactataggc tttcaggaat accgccgagt
     7621 tgatctggtg agaaatatca taaccctgaa caacgccatg gggataatca gcaaccgcaa
     7681 ttttcatcca gtaattctgg cagggagtaa catcaatgcc ttctgataca aaaggaaata
     7741 tctggcgtgg cagggatgcc ccgtcaaaaa taaaactcct gtcgtagttg ggttggtagt
     7801 accctggagg ggtagtatga ttcagggttt tgacacatac cggaatccag gaaggatctc
     7861 catcctgccc gataagggaa gcaatcatgt tgtagttttg ctcgctgggg tatcttttaa
     7921 taaaaacagc cataaaatcc gagacaggcg ctccggtcat atcgacgtct atcggcggtg
     7981 gaggcggtgg tggaaaaggt ggctgctcgt agagatattc ttcagaagca aatacatatt
     8041 ccagttttat agagttgtaa tagggtttga acttaaaagt aataatagaa gggtcatatg
     8101 ccgtatccgg tttcggatcg ttgggagtaa acaatccgga gagccagtcc atatcatcat
     8161 cctgtagcgg cggcggcccg ttgggctcag cccggtaagg ccccaggctg tcgtgggcgg
     8221 gtatagcctg tgctccggtt ttattctggc caccatcaac aatagtagtc gtacccaccc
     8281 acccatttga gaggatcaac ccgtcattca tcccgatatc cgcaccatcg gtaaataaac
     8341 cgacaccttt gggatttccg atatattgaa tgtcggagat acgcgctccg ccaagaaaca
     8401 gcgtatcttc aatatacttt ataatatcga tcagataaag ggaatccggc agcggagaat
     8461 cgggattgat catcttataa atattcaatt cctgtgttcc ctggggatag aggagtgtgg
     8521 gcagtaaaac caggctcaat cctactaaaa gctttttaaa cttttgaatc gggttggtgg
     8581 tttttaaaaa attcaagatc attttgaaaa tgttttcttt atgtgaaata ataaacctgt
     8641 aaagttaaac acactttttt aatatgtgta tttcaaatgc taaaatttga ctttaggctc
     8701 tgaaaattca atcaaaacaa ccgggacctg ccaggctggc tcaattttcc tgataacttt
     8761 atatttcatg tttcgcaagt gttcccattg cgccaggtat agcgctccgg gcaatttctc
     8821 cgttttcact tccgtaatcc gtcccctggc agttgaaaga tagaaagtga atatctcgtt
     8881 attatacctg tactgaaaat aaggatcggg tttgaatgag ggattcactt tcaatgagag
     8941 aacccatgca tactgctgtt tctattatca cttttgtctt gctg
//
LOCUS       DAOPNH010000132         8942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8599_length_8942_cov_4.778778, whole genome shotgun sequence.
ACCESSION   DAOPNH010000132 DAOPNH010000000
VERSION     DAOPNH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8599_length_8942_cov_4.778778"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PKZ74_08195"
     CDS             complement(<1..1755)
                     /locus_tag="PKZ74_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPM92777.1"
                     /translation="MPLTIKEEPMMKYLHTFVFSLILVMILSSCTESVNKEPLIGKKE
                     LKLATDRMTPEVLWSFGRVGEFEVSPDLQKILFSITYYDIDRNKSNRELYLIGTDGKN
                     LKQITKTPENEFNYTWRPDGQKILFAGTASGSVQVWEMNPDGSGRKQLTDIPGGISGF
                     KFSADMKKIAYSAEVNLDDQYKKDLFEGLPLTSGKVYHSMMFRHWDEWRDTYSHLFIA
                     DFQDGKLSNVTDILEGEPFDFPNKPFGGMEQMSWHPDGKKLAYSCHKNFGKAYAVSTN
                     TDIFIYDLETRQQTLLTRDMKGYDNDPVFSPDGRHIAWTSMERDGYEADKSRLMVFDL
                     ESNSRVDYTGIFDNDAEGPVWADDSQSIYFVAYWYGSKEIFNLHLNGTITQLSSGVHD
                     FTAAQPAGDKIIASMTTMSMPAELFAVPAAGGKETQITFTNKDILDQLSLGKVEKRFV
                     NTTDGKKMLTWVIYPPHFDERNKYPALLYCQGGPQNALSQFWSYRWNFQMMAANDYIV
                     VAPNRRGVPGFGQAWKEQISGDYGGQNMQDLLAAIDDVSNEGYVDKDRLGAVGASYGG
                     FSVYWLAGHHERRFKAFIA"
     gene            complement(1765..3966)
                     /locus_tag="PKZ74_08200"
     CDS             complement(1765..3966)
                     /locus_tag="PKZ74_08200"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPM92778.1"
                     /translation="MRKKPGRSSKNTWPENNLSLIRKLTTTGLGACISVFLSAQNLLP
                     ADGYLIINAGPGDPVYTTYTAAPARSSLYDDKGYTMIYDEFSSPLTYGSEHGGRFFTV
                     WEINSVVVSRVGDFHVKPFIKATFPDMALMEYEPWPGISAEEIFFVYNSNCAVVDCHL
                     KNNTPNTHEINIYPVLEDADSLYVIGFDSTSNSYCFNHYETKKRLISNLSDKYPYPDH
                     FYNLFSAGFPIYSYGAYQGNREDLYNAIKTDFYIENRSDSLNMADSSDAGFIALHGKY
                     ILQPGATIHYRYCRLRQSRDEEPERLGKILEDAKSLSIQPFIDENVRLFSNIPLIDFS
                     DPADKMVYLSAFNLARGCFLPAEGQTTHNYYVFSREPLWGWGHGHQVLHESLSMLAYA
                     YLDPVSAQNSQRVYMEQQSEDGLIAYRHGPRGPQTYPHKEMPTTSAPFYSWINLEIYN
                     VSHDREFLADAYESGTRYARWLINNRDTDHDGTFEWGPYGIIENVRDWYNAVFQVSKE
                     RYLDIDKEDISDELECLDLSLMMVKEMRSLAAMATALDLREESEEWESKANKLARLIN
                     ERMWDDASGFYYSVDKTDHSFYFMTRDLKRQEIIGFLPLWTQVADSTRAVRLVQTLTD
                     PGKFWRKYGVPTLAADDEWYSPYVDYCCKWNGPVWLLWDYMVYEGLRNYGYDSLAEEL
                     AVNLLGAVKVQLSRNHRFPESYSPDNEVLNCPPNYIWDSIIARLMIRMNSD"
     gene            complement(3917..4153)
                     /locus_tag="PKZ74_08205"
     CDS             complement(3917..4153)
                     /locus_tag="PKZ74_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPM92779.1"
                     /translation="MSEQESLKNVFTGSVLEANYIKSLLEESGIGAMVRNTMGESLVA
                     GWVSGGPEDAGLVYVIESHEEEARKIVEEYLAGK"
     gene            complement(4209..5135)
                     /locus_tag="PKZ74_08210"
     CDS             complement(4209..5135)
                     /locus_tag="PKZ74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM92780.1"
                     /translation="MPGRKAFIIGATAISFAAIMWGLDGVVLTPRLYNLDVGLVVFIL
                     HLIPFLLMQLFLFREYRHLKTFRRNDYILLLLIALFGGAIGTLSIVKALFLINFKELS
                     IVVLLQKLQPVFAILLATLLLKERINRYFILWASVALLGGYFMTFGFKIPDVHTGSNT
                     ALSALFALLAAFSFGSSTVFSKMILRRYSFSTATFYRYGFTTVIMLLYVIITGQLASY
                     HEITRSNWLIFIIIAVTTGSGAIFLYYYGLNRVKATVAVICELFFPISTLVFDYFING
                     TRLSAVQWASAALMVFAIINLNRTQQAENTRS"
     gene            5216..5641
                     /locus_tag="PKZ74_08215"
     CDS             5216..5641
                     /locus_tag="PKZ74_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPM92781.1"
                     /translation="MVEDKWITLRNIARIEVRVGDVNYGGHMGNDRALLVFHDARIAF
                     LESLGFSEKNIGGPAIIMRDAHVNFRKEVFLHDVLTVDVGIDEVTTSSFNMTYTVKRE
                     SDGAVVFLGSTGLVAFDYETRKVVKLPEVFIQRIVKSEE"
     gene            5805..7196
                     /locus_tag="PKZ74_08220"
     CDS             5805..7196
                     /locus_tag="PKZ74_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM92782.1"
                     /translation="MKNLLAILFLFAVGGLAAQVTFVIDALPEYTPPDDYIYIAGDFN
                     GWTPGDPNFRLAKNEQQKWAITLAAATQGTQIQFKFTRGSWETVEKGQSGEEIGNRLF
                     TYGNGSTVNIVIYNWAQGGGGGSTAAENVIIMDDAFEMPQLGRTRRIWLYLPPGYETS
                     ENNYPVLYMHDGQNLFDDATSFAGEWHIDETLNDLFDQGINVPIVVGIDNGGAERLNE
                     YTPWKHVVHGGGDGELYMQFITETLKPYIDGHYRTLPGREHTGIMGSSLGGLISHFGA
                     LDNQDIFSKAGIFSPSYWFSDSLWAFTREAGKQDAMRLYLMCGGMEGQNTINDVMDMQ
                     DSLLVSGYQEEDLSVKIIPGGQHNENLWSGDFGEAYLWLFAAYANDITEHVRYEVIRL
                     YPNPATGMVSFPADFPRDIDSVDIIDMQGVLVNRIEPVTGREIDISGLSNGIYVILVR
                     TGNTWYQGKVVKE"
     gene            complement(7229..7861)
                     /locus_tag="PKZ74_08225"
     CDS             complement(7229..7861)
                     /locus_tag="PKZ74_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPM92783.1"
                     /translation="MEIGNWKFGNQEPGTRNLELFHPTNDQRKILILPVSPLFSQISL
                     MTMVNRRALVPLYVLGIMFYVLIINDVKVVAQDGAIRNEILMEVNKLRKEGCTCGEEK
                     MPPVMEVTWNDKLEKAAAKHADDLFKNSRFSHMGNDGSEASDRVEKAGYKWSAVGENI
                     GKGYATAAQVVNGWRQSPGHCRNMMDPSFREIGAARKGEIWVLNLGAWRE"
     gene            8185..8586
                     /locus_tag="PKZ74_08230"
     CDS             8185..8586
                     /locus_tag="PKZ74_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM92784.1"
                     /translation="MKLKVIIERGNDGSFGAYIGSDNAPFGIIGDGKTALEAKTDFLN
                     SYEEMKAYYMETNKEFTECEFEFQFDVASFLAYYGCILSLAGMERLTGVNQGQLSHYV
                     TGRRKPSKKTIEKIEKKLHEFGRELNQVEFI"
BASE COUNT         2383 a         2101 c         2146 g         2312 t
ORIGIN      
        1 cgcaataaaa gctttgaacc gtctctcatg atgtccggcc agccagtaaa cggaaaatcc
       61 gccataactg gctccaacag cgcccaggcg atctttatcg acataaccct cgtttgaaac
      121 atcatcgatc gccgccagca aatcctgcat gttctgtccg ccatagtcac cgctgatctg
      181 ttctttccag gcctggccga atcccgggac gccccggcgg ttcggggcta ccacgatata
      241 gtcgtttgca gccatcatct gaaagttcca gcggtacgac cagaactggc tcagggcatt
      301 ctgcggacca ccctggcaat aaagcaatgc cgggtatttg ttccgctcgt caaaatgggg
      361 cggataaatt acccaggtca gcatcttctt accatccgta gtgttgacaa accgcttctc
      421 aaccttcccc agggagagct ggtcgaggat gtctttattg gtgaaggtga tctgtgtctc
      481 ttttccgccg gcagcaggta cggcgaaaag ctcggcaggc atcgacatgg tcgtcatcga
      541 agcaatgatc ttgtcgcctg caggctgggc agcagtgaaa tcgtggacgc cactgctgag
      601 ctgtgttatc gtaccattga ggtgaagatt aaaaatttcc ttggatccgt accaataagc
      661 aacaaaatag atcgactggc tgtcgtcggc ccataccggg ccctcggcgt cattatcgaa
      721 aattcccgta taatcgacgc gtgaattgct ctccaggtcg aaaaccatta accggctctt
      781 atcggcttca taaccgtcac gttccatact ggtccaggca atgtgtcttc cgtcaggcga
      841 gaatacagga tcattgtcat aacctttcat atcacgtgtc aggagggtct gctgcctggt
      901 ttccaggtca taaatgaaga tgtcggtatt ggtggaaaca gcgtaggctt ttccgaaatt
      961 cttatggcag gaataagcca gctttttccc atcggggtgc cagctcatct gctccatgcc
     1021 gccaaaaggc ttgttgggaa agtcaaacgg ttccccttcc agaatatccg ttacgttgga
     1081 taattttccg tcctggaaat ccgcgataaa aagatgactg tatgtatcac gccattcgtc
     1141 ccaatgcctg aacatcatcg aatgataaac cttcccggag gtcaacggta atccttcaaa
     1201 caaatctttc ttgtattgat catccaggtt tacctcagcc gagtaagcta ttttcttcat
     1261 atcggccgaa aatttaaagc cgctgatgcc gccggggata tccgtaagct gctttctgcc
     1321 cgatccatcg ggattcattt cccaaacctg aacagaacca ctggctgtgc cggcaaacag
     1381 gatcttctgc ccatccggcc gccacgtgta gttgaattcg ttttcgggtg tttttgttat
     1441 ctgcttcaga ttcttgccgt cagttccgat caggtatagt tcccggttac ttttattcct
     1501 gtcaatatca taataagtga tgctgaacag tattttctga aggtccggtg aaacttcaaa
     1561 ctcgcccaca cgcccaaacg accataatac ctccggcgtc atacggtctg tggccagttt
     1621 taactctttt ttcccgatca gaggttcttt gttaacggat tccgtgcacg atgaaaggat
     1681 catcacaagg atcaggctga aaacaaaggt gtgcaagtat ttcatcatcg gctcttcttt
     1741 tatagtgagt ggcaaaatta agaattaatc gctattcatt ctgatcatca accgggcaat
     1801 gatcgaatcc cagatgtaat tgggggggca attcaggact tcattgtccg ggctgtatga
     1861 ttccgggaac cggtggtttc tcgaaagctg cactttaaca gcacccagta aattcacagc
     1921 cagttcttcc gccaggctgt catagccgta attcctcaac ccttcataaa ccatgtaatc
     1981 ccataacagc cagacgggcc cgttccactt gcagcaataa tcgacataag ggctgtacca
     2041 ttcatcatcg gcagccagcg taggcacgcc gtatttcctc cagaatttgc cgggatcggt
     2101 caatgtttgc accagccgga cggctcttgt gctgtcggca acttgtgtcc agagcggcag
     2161 aaaaccgatg atctcctgcc gtttgaggtc cctcgtcata aaatagaagc tgtggtcagt
     2221 cttgtcgacc gaataataga atcccgacgc atcatcccac attctttcat tgatcaatcg
     2281 ggccagtttg tttgctttac tttcccactc ctcactctct tctcgtaaat caagcgctgt
     2341 agccatcgcg gccaggctcc gcatttcctt aaccatcatc aggctgagat caaggcattc
     2401 gagctcatcc gaaatatcct ccttgtcgat atccaggtaa cgctccttcg aaacctggaa
     2461 tacggcatta taccaatccc tcacattttc aatgattcca tatgggcccc attcgaaagt
     2521 cccgtcatga tccgtgtccc ggttattgat cagccaccgc gcataccggg tgccgctctc
     2581 gtaagcgtca gcaagaaact ccctgtcatg gctcacatta taaatctcca ggtttatcca
     2641 cgaatagaac ggagccgatg tggtgggcat ttctttatgc ggatatgtct gcggcccccg
     2701 aggtccgtgg cggtaagcaa tcaaaccgtc ttcgctttgc tgttccatat atacccgttg
     2761 tgaattctgt gccgaaacag gatcaaggta ggcataggcc agcatgctga gcgattcatg
     2821 caggacctgg tggccgtgtc cccagcccca gagcggttcc cgtgagaaaa cataataatt
     2881 atgggtagtc tgtccttcgg ccggcaaaaa gcagcccctg gccaggttga aagccgaaag
     2941 gtaaaccatc ttatccgcag gatcactgaa gtcgatcagt ggaatattgg aaaacaatct
     3001 gacattttca tcaataaatg gctgaatgga aaggctcttt gcatcttcca ggattttgcc
     3061 aagacgttcg ggctcctcgt cccggctctg tcgcaaccgg caataacggt aatggattgt
     3121 agcgcccggt tgaaggatgt attttccatg cagggctatg aaaccggcat cactgctgtc
     3181 tgccatattg agggaatccg acctgttttc aatgtaaaag tcggtcttga tggcattgta
     3241 cagatcctcc ctgttacctt gatacgcacc gtaagagtat attgggaacc cggctgaaaa
     3301 aaggttataa aaatggtcgg gataaggata tttatcggat agattgctga tcagcctttt
     3361 ttttgtttcg taatgattaa aacaataact atttgaagta gagtcaaatc caatgacata
     3421 aagggaatcg gcatcttcca gtacggggta aatattgatc tcgtgggtgt ttggtgtatt
     3481 gtttttcaga tgacaatcca caacagcaca attcgaatta tatacgaaaa agatctcttc
     3541 agcgctgatt ccgggccatg gctcgtattc catgagcgcc atatccggaa aagtagcttt
     3601 gatgaacggt tttacatgaa aatcgccgac ccggctgact accacgctgt tgatttccca
     3661 aacagtgaaa aaccttccgc cgtgttcact gccgtacgtt aaaggggatg agaattcatc
     3721 atagatcatc gtataaccct tgtcgtcgta aagagatgag cgtgccggcg ccgctgtata
     3781 tgtagtataa acaggatcgc cgggaccggc attgatgatc aggtacccgt cggcaggaag
     3841 aagattctgg gccgacagga aaacagatat acaggccccg agacctgtcg tggttagctt
     3901 ccgtatcaac gataaattat tttccggcca ggtattcttc gacgatcttc ctggcttctt
     3961 cctcatggct ttcaataaca taaaccaggc cggcatcttc cgggcctccc gaaacccagc
     4021 cggcaacaag gctttctccc atggtattgc ggaccatcgc gccgataccg ctctcttcca
     4081 gcaaactctt aatataatta gcttccaata cggaaccggt aaatacattc tttaaacttt
     4141 cctgttcgct catagctcat agttttaagt cttagacaaa tataaggaaa aagcaaagaa
     4201 aagcaaaatc atgaacgtgt attttcagcc tgttgtgtac ggttcaggtt gatgatggca
     4261 aataccatca gggcggcgct ggcccattga accgctgaaa gcctggttcc attgataaaa
     4321 taatcgaaga ccagggtcga gatagggaag aataattcgc aaatcacggc tacagtggct
     4381 ttgacccggt tcagcccgta ataataaagg aagatggcac cgctgccggt cgtcactgca
     4441 atgatgatga atatgagcca gttggagcgg gttatttcat gatatgaagc cagttgcccg
     4501 gtaatgatca catacaacag catgatgaca gtggtaaacc cgtaccggta aaaagtggcg
     4561 gtactgaatg aatacctcct caggatcatt ttgctgaaga cggtggaact gccgaacgag
     4621 aaggctgcca gaagggcaaa caacgctgat aatgcagtat ttgacccggt gtgcacatcc
     4681 ggaattttga agccgaatgt catgaaatag ccacccagca aggcaaccga tgcccagagg
     4741 ataaaatatc ggttgatcct ctctttcaat aacaacgtgg ccagcagtat ggcaaacacc
     4801 ggctggagtt tctgtagcag gaccacgatg cttaactcct tgaaattgat cagaaagagg
     4861 gcttttacaa tggataaagt accgattgca ccaccgaata aggctatcag cagtaataaa
     4921 atataatcat ttcgccggaa tgttttcagg tgacggtatt cccggaaaag aaaaagctgc
     4981 attaacagga acgggatcag gtgcaggata aaaacgacaa gcccgacatc caggttgtac
     5041 agcctgggcg ttagcacaac accgtccagg ccccacatga tggctgcaaa actaatggcc
     5101 gttgccccga taatgaatgc ttttcttccc ggcatgattc cctgaatagg gctgcaaaga
     5161 tagttttaag tcttaaatat tgtatttttg atgataatga tatgatgaaa agaatatggt
     5221 tgaggataaa tggattaccc tgaggaatat tgccaggatt gaagtcaggg tcggagatgt
     5281 caattatggc ggacatatgg gaaacgacag ggcattgctg gttttccacg atgcccggat
     5341 tgcctttctg gaatcgctgg gattcagtga gaaaaatatc ggcgggccgg ccatcatcat
     5401 gcgcgacgcc catgtgaact tccgtaaaga ggtgttcctg cacgatgttt taacggtgga
     5461 tgtcggtatt gatgaggtga ccacatcttc attcaatatg acctataccg taaaacggga
     5521 aagtgacggc gcggtcgtat tcctgggcag caccggcctg gtggcatttg attatgaaac
     5581 acgaaaagtg gttaaactgc cggaagtctt tattcaacga atagtgaaaa gtgaagagtg
     5641 aaaagtgaag agtgaagagt gaagagtgaa gagtgaaaag tgaattggtt aacaatagaa
     5701 caatagaaca atagaacaat agaacaatag agcaatagaa caatagagca atagaacaat
     5761 agaacaatag aacaatagaa caatagaaca atagaacata taccatgaaa aacctgcttg
     5821 cgatactgtt tttgtttgct gtcggtggcc tggcggcaca ggtgactttt gtaatcgatg
     5881 cattgccgga atatactcct cctgacgact acatttatat cgccggtgat tttaacggat
     5941 ggacccccgg tgatccgaac ttccggctgg cgaagaacga acagcagaaa tgggcgatca
     6001 cgctggcggc ggcaacacag gggacccaga ttcaattcaa gttcacccgc gggagctggg
     6061 agacggttga aaaagggcaa agcggggagg agatcggaaa caggctcttc acctacggca
     6121 atgggtcaac ggtgaatatc gtcatttaca actgggcgca gggaggtggt ggaggcagta
     6181 cggctgccga aaatgtgatc atcatggatg atgcctttga gatgccgcaa ctgggccgca
     6241 cccgccggat ctggctttac ctgcctcccg gttatgagac ttcggaaaac aactacccgg
     6301 tgttgtatat gcacgatggc caaaaccttt tcgacgatgc cacttccttt gccggggaat
     6361 ggcatatcga cgaaacatta aatgatcttt ttgatcaggg gattaatgtc ccgatcgtgg
     6421 taggtatcga caatggcggc gcagagcggc tgaatgagta cactccctgg aagcatgttg
     6481 tgcacggagg aggcgatggc gaactctaca tgcagttcat cacggaaacg ctgaaacctt
     6541 atatcgacgg gcactaccgc actttgccgg gccgagagca taccggcatc atgggcagtt
     6601 ctctcggcgg cctgatctcc cattttggag cgctggataa ccaggatatc ttcagcaaag
     6661 ccggcatctt ctctccctct tactggttct ccgacagcct gtgggcattt acccgtgaag
     6721 cgggaaagca ggacgccatg cgcctttacc tgatgtgcgg cggcatggag gggcagaata
     6781 caatcaatga tgtgatggat atgcaggaca gcctgctggt aagcggatac caggaagagg
     6841 atctgagcgt gaagatcatc cccggcgggc agcacaacga aaacctctgg agcggcgact
     6901 tcggcgaagc ctacctctgg ctgtttgcgg cctatgccaa cgatataacc gaacatgtcc
     6961 gttatgaggt aatccgccta tatcccaatc ctgccacagg catggtatct tttcctgcag
     7021 attttccccg ggatatcgac agcgtggata taattgatat gcaaggtgtc ctggttaacc
     7081 gaatcgagcc ggtgacagga agagaaattg atatctcggg attatcgaat ggaatctatg
     7141 tgatcctggt gagaactggc aatacctggt accaggggaa agtggtgaag gagtgaaaat
     7201 tgtgctttac ttgatgttcc gttttgtgtt attccctcca cgcgccaagg tttaaaaccc
     7261 agatctctcc tttccgggcc gctccgatct ccctgaagga tggatccatc atattccggc
     7321 agtgccccgg gctctgtcgc catccattca ccacctgggc cgcggttgca tatcctttcc
     7381 ctatattttc tcccacagcc gaccatttat agcctgcttt ctcaacgcgg tccgatgctt
     7441 cggagccatc attgcccata tggctgaacc gcgaattttt aaaaagatca tcggcatgtt
     7501 ttgcagcagc tttttcaagc ttgtcgttcc aggtaacctc catgaccggc ggcatctttt
     7561 cctctccgca agtgcatcct tctttgcgca gcttattgac ctccattaga atttcgttcc
     7621 gtatcgcccc atcctgtgct actaccttta catcattaat aatcaacaca taaaacataa
     7681 tacccagcac atacaaaggc accagagcac gacggttcac catagtcata agcgatattt
     7741 gagagaataa cggtgaaacc gggaggatta gtatttttct ttggtcgttg gtcggatgaa
     7801 agagttccag gttcctggtt cctggttcct ggttgccaaa tttccaattt ccaatttcca
     7861 ttttttctcg aagaggttcg gcgattttca gaggcccaaa gtctggtatt gaccggaaag
     7921 tgaaggaata gctccgggct ccggcccgaa aagcgcataa aacaaaacta atttgtttgt
     7981 taaatcttta ccccgtaggg gttttccgtc ttgtagaaat cgtggccggt tatggtaaat
     8041 tccccctccc tttgatgccg tttcctaaag ttgcagtgtt tcacagggag gggcagggga
     8101 gggtgaaaaa ataatggaat tttaataatt gatattaata ttaatatctt tataatctct
     8161 ggtagaagtt tagtatttga ggatatgaag ttgaaggtaa ttattgaacg cggtaatgac
     8221 ggcagtttcg gagcctatat tggttctgac aatgcgcctt ttggaatcat aggagatgga
     8281 aaaactgcgc tggaagcaaa gacggatttt ttaaattcgt atgaagagat gaaagcatat
     8341 tacatggaga caaataaaga gtttactgaa tgtgaatttg aatttcaatt tgatgtggcc
     8401 tcatttttag cttattatgg atgtatctta tccttagccg gcatggaacg gttaactggt
     8461 gtaaatcagg gacagttaag ccattacgta acaggccgcc gaaaaccgtc aaaaaaaacc
     8521 attgaaaaga ttgaaaagaa attgcacgag tttggccggg aattaaacca ggtggagttt
     8581 atatgaaata ccgattcagt cgtcggtcgt cagtcaacta ttaactcaaa acccctccaa
     8641 gtcccatagg gacgccatta tggtagaatg ttggtgacag gatttcccat ttcccctccc
     8701 tttgatgccg tttcccaaag ttgcagtgtt tcacagggag ggtgagggga gggtgattca
     8761 tagaattgat tcttaagtga ttatttcgga caaacaaggc cacctgtttc ggtgtaaatt
     8821 gagccactca tttcggacga aagtgagtct ctatttcaaa gggcaacatg agggtaatag
     8881 tattaattta tttccattga ttttcttctt aacgattctc ctttaatttc aaatcggtgt
     8941 gc
//
LOCUS       DAOPNH010000133         8901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8646_length_8901_cov_6.817997, whole genome shotgun sequence.
ACCESSION   DAOPNH010000133 DAOPNH010000000
VERSION     DAOPNH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8646_length_8901_cov_6.817997"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKZ74_08235"
     CDS             <1..837
                     /locus_tag="PKZ74_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92785.1"
                     /translation="ARVEIFCEEKVSNELIRFIKALPPKLDFQEEGNAIQRICQLLYK
                     KNLDYEIVEQKVYDDLRRVVSSGYKLLKFVSNSNPLPIPSSFVASDNLDFIKSWREVL
                     MKVFNNSPDFTKISSVSYCASFPNLNFEIYSKSVDVNFYAAIWMSLQHALLNRPGMVT
                     KDGKIYLWVTNAIFVEIRIRKQMNSRQFAGLFNVKRKDSNNRVSYKDQADITNIIGGY
                     TSSGYYIPYGVYIIDKKISVEIYFYQNALLQDSISITALANQNSSASLVEDRRKADDD
                     DD"
     gene            complement(1127..2344)
                     /locus_tag="PKZ74_08240"
     CDS             complement(1127..2344)
                     /locus_tag="PKZ74_08240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM92786.1"
                     /translation="MNRVVITGMGIYSTIGKNLEEVRESLYTGKSGIGFEPARKEMGF
                     RSGLTGILERPQLKGLLDRRQRIGMAEQAEYAYISTVEALRLAGIDQDYLDKVEAGIL
                     FGNDSSAVPVIETIDIMRDKKDTALIGSGLIFQTMNSTITMNLSVIYKLKGINLTVSG
                     ACASGSHALGLAYYLIRSGLQECIIAGGGQEVNLESTGNFDALNAFSVRDGEPTKASR
                     PFDRDRDGLVPSGGAATLILESYDSAVRRGAKILAEVIGYGFSSNGDHISVPNLDGPV
                     KAMRMALKDAGLSAKEIGYINAHATSTPVGDAKEAEAIDLVFGDAKPWVSSTKSMTGH
                     EMWMGGASEVIYSILMMQNNFIAPNINFENPDEYSAKLKIADRTIHQDFNIFLSNAFG
                     FGGTNSTLIVRGL"
     gene            complement(2412..3143)
                     /gene="fabG"
                     /locus_tag="PKZ74_08245"
     CDS             complement(2412..3143)
                     /gene="fabG"
                     /locus_tag="PKZ74_08245"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPM92787.1"
                     /translation="MKKYALVTGGSRGIGKATCIRLAEMGYPVIINYRSNEEEARQTM
                     AAVVGNGGEAELLPFDVSSQEAAEKALDLWMEAHPDDYIAILVNNAGIRKDNLLVFMN
                     DEEWNSVMNTNLGSLFFITRKLIKSMMVNKWGRIINVVSLSGIKGLPGQVNYSAAKAG
                     VIGATKALAQEVAKKNITVNAVAPGFIRTDMTKDLDENQHKPLIPMGRFGNPEEVAEA
                     VAFLAGERTGYITGEVISVNGGLYT"
     gene            complement(3147..4682)
                     /locus_tag="PKZ74_08250"
     CDS             complement(3147..4682)
                     /locus_tag="PKZ74_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPM92788.1"
                     /translation="MIVIGGRDICLEDFQQIIFGQETVSLDPEAFSAVNDSFLFLKDF
                     SKNKIIYGINTGLGPMSQYKVQEKDRQALQYNLIRSHAAGMGEPISEIHVKALMVARL
                     NTLMKGYSGIHPSVVELLRDFINHDITPVIPRHGGVGASGDLVQLAHLALALIGEGEV
                     TVRGTRMATEEALRKAGLEPVKMHIREALSLINGTSVMSGIGIVNVIRAKRLLQWAVA
                     ASALINEVVESYDDHFSLELNRVKLHPGQQEITGMLQNILADSRLTRKRAEDLFNGTN
                     GETVFTKKIQEYYSLRCITQILGPVADTIHAAERVVLNEINSVNDNPVIDHRNGNVFH
                     GGNFHGDYISLEMDKLKLAITKLSMLAERQLNFLMNDKLNGKLPPFVNLGTLGLNLGM
                     QGTQFTATSTVAENQSLSNPVYIHSIPTNNDNQDIVSMGTNAALLTGRVIRNSFEVMS
                     IHLLTLMQAVDHLKIRNRMSTFTGGIYDALRARVPAFADDHYMSPDLRKINEYISNNS
                     LNI"
     gene            complement(4797..5483)
                     /locus_tag="PKZ74_08255"
     CDS             complement(4797..5483)
                     /locus_tag="PKZ74_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPM92789.1"
                     /translation="MISIPMLIIFGVFMLLSWLISMQLKSKFKKYSTVPINQNLTGKE
                     VAEKMLRDNGISDVKVVSTPGMLSDHYNPLTKTVNLSEGVYASSNVAAAAVAAHECGH
                     AVQHATAYNWLTMRSKLVPVVSFASNWVQWVLLAGVLLIEVFPQLLLLGIFLFALTTL
                     FSFITLPVEINATNRALVWLNTSGITNVQNHDKAQDALRWAAYTYVIAALGSLATLLY
                     YIMIFLGRRD"
     gene            complement(5584..7275)
                     /locus_tag="PKZ74_08260"
     CDS             complement(5584..7275)
                     /locus_tag="PKZ74_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM92790.1"
                     /translation="MKNLSGCIIWYLFIFNCLNIHAQTIIPGGYVSGNWDISGSPYLI
                     DGNLLVQPDSTLEIGLGTEILFRGTFRLEVLGQLLVSGTPTQPVIFDGENDTIEWRGI
                     YFNATDSSLTDSSILANGSINNSLGGSGLTLNNSNRVRISGFTIRNCASFQGGAIRCV
                     DSAPVFENLLIENNSALDGAGIALDASNAIIKNCSFNNNIANGAGGGMVIYNGSAPVL
                     ENCTFTGNSSIGSGGGIYINDASPVLSQCTFLANAGALGGSSLYSGGAVSVKLGADPR
                     FENCTFSENTSNTSGGAIASFSPNEIINCLFVENSAPVSGGAIFLSSGNPIETTLFNN
                     TFHNNESPDGSVLYAHNHVAVMLNCIAWQDETPDTSSLVRLDAIMSLVALFIAYSDLK
                     DAQEGIELTGNAGYSWGTGNIVLDPLFLPGIYDLDWQSPCIEAGTPDTTGLGLPELDL
                     NNNPRYANDRIDMGAFEYQLPLAVQSLKFKDQREMRVFPNPAREWVWVETNEDREYCS
                     IELINSSGLILSKKDLHPGEEMARIDLSPYPAGIYLVRLSANGEGVSNRSIIKVR"
     gene            complement(7548..>8901)
                     /locus_tag="PKZ74_08265"
     CDS             complement(7548..>8901)
                     /locus_tag="PKZ74_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPM92791.1"
                     /translation="NQNASVRETFNTALGYEALRGSTSAASNSGQYNTAIGDAALWTN
                     SMGNHNTSVGAQALYQNTQGEQNTAMGFGALYNNKENDRSTAVGYMAMYNADNRTTSE
                     RSTYNTAVGFQALSGSSTPSANTGQENTAVGDQALSLNASGSFNTGCGAFALQFNDAG
                     NYNTAVGNMSMVSNNGGSYSTAVGYQAMYATTTGGSNLAVGYRSLYSNVGKRRNTVVG
                     IEAMFNADDTPTSGDAYNTAIGYKALYGSGTPASNTGTSNTAVGDQALFGNTSGNNNV
                     ALGRNALDGNTVGSNNTAIGYEADVAFNNLTNATAIGYGASVNASNKIVLGNSSATTV
                     GGYGTWTNYSDARLKENISYKDDLGLDFVMKMRTASYNYIDDENKRRRDGLIAQDVQQ
                     ALDELGVEFSGLVVDDDEMKTLNLSYGEFVIPLINAVKEQQAQIEALRKEIEALKADR
                     "
BASE COUNT         2153 a         2200 c         2191 g         2357 t
ORIGIN      
        1 gccagagtcg agattttttg tgaagaaaaa gtttctaatg aattaatcag gtttattaaa
       61 gctttgcctc ccaaacttga ctttcaagaa gaaggtaatg ccatacagag aatctgccaa
      121 ctattatata aaaagaactt agattatgag atagtagagc aaaaggttta tgatgacctc
      181 aggagagtcg tgtcaagcgg atataaatta ttaaaatttg tatcaaattc taatccattg
      241 cccattcctt cttcatttgt tgcatctgat aatcttgatt ttattaaatc ttggcgggaa
      301 gttttgatga aagtctttaa taactcacct gattttacta aaatatcttc tgtaagttat
      361 tgtgcatctt ttccaaactt gaattttgag atttactcta aatcagtcga tgtcaatttt
      421 tatgcagcaa tttggatgag tttacaacat gcattgctaa ataggccagg aatggttaca
      481 aaagatggta aaatatatct ttgggtgacg aatgccatct ttgtagagat tcgtattcgg
      541 aaacaaatga atagtcgcca atttgctgga ttattcaatg tgaaaagaaa ggatagcaac
      601 aacagggtct catataaaga tcaggcggat ataacaaata taattggagg ttataccagt
      661 tctggatact acatccctta tggtgtatat attatcgata agaaaataag tgtagaaatc
      721 tatttctatc agaacgcact tttgcaggat tcaatatcta ttactgcatt ggctaatcag
      781 aatagttcgg caagtttagt tgaggatagg cggaaggctg atgatgatga cgattgattg
      841 atgattgatt tcatgtttct tttaagaatt atgtattttc aatacttacc caccccttga
      901 tcccctccca tgccaatatt tgaggatcga tcccggatta ccgggggagg ggaaaagggt
      961 ggtgattcca tccttgtgct acaatattgg atcacctacg gtgaaggttc cagattcaat
     1021 aaatgacacc aatgacgctc gcgcagcgaa ctaatgacgc gaacgtagtg agcttatgac
     1081 acgaacaaag tgagcttatg acgccctttt gacctccgac caatgactaa agacctctaa
     1141 caatcagcgt gctgttcgtg ccgccgaatc cgaaggcatt ggaaaggaaa atattgaaat
     1201 cctggtggat ggtacgatcg gcgattttca gttttgccga atattcatcg gggttttcga
     1261 agttgatgtt gggggcgata aagttgttct gcatcatcag gatggaatag atcacttcgc
     1321 tggcgccgcc catccacatt tcatggcctg tcatcgattt ggtgctgctg acccagggct
     1381 ttgcatcgcc gaatacgaga tcgatcgctt cggcctcttt cgcgtcgcca acgggggtgc
     1441 tggtggcatg ggcgttgatg tagccgatct cttttgcgga taggccggcg tctttcagcg
     1501 ccatccgcat cgctttcacc ggcccgtcga ggttgggaac agagatatgg tcgccattgg
     1561 aggagaagcc gtaaccgatc acctcggcca ggatcttcgc gccgcgcctg acggctgagt
     1621 cgtagctttc caggatcaac gtggccgccc cgccgctggg caccagcccg tcgcggtcgc
     1681 ggtcgaacgg gcggctggct tttgttggct cgccatccct gaccgaaaaa gcattcagcg
     1741 catcgaagtt tccggtcgat tcgagattga cctcctgccc gccgccggca atgatgcact
     1801 cctgcaatcc gctgcggatg aggtagtatg ccagccccag ggcatgcgat ccgctggcgc
     1861 aggcgccgct caccgtaaga ttgatccctt tcagcttgta aatcaccgaa agattcatcg
     1921 tgatggtcga gttcatggtc tgaaagatca gccccgaacc gatcaaagcc gtatccttct
     1981 tgtcgcgcat gatgtcgatg gtctcgatca ccggtacggc cgagctgtcg ttgccgaaca
     2041 ggatgcccgc ttccactttg tcgaggtaat cctggtcgat accggccagc ctgagcgcct
     2101 caactgtgct gatgtaggcg tattcggcct gctccgccat gccgatccgc tggcgccggt
     2161 cgagcaaacc tttcaactgc ggccgctcca ggatgcccgt caggcctgag cggaaaccca
     2221 tctctttgcg ggccggctca aacccgatcc ccgattttcc ggtataaagc gattccctca
     2281 cctcttcgag attcttcccg atggtggagt agatgcccat tccggttatg acaacgcgat
     2341 tcatatgata gttaaaagtt caaaattcaa agttcaaagt ttacaaagca ttttgcactt
     2401 tgcactttga actatgtata tagtcctcca tttactgaaa taacttctcc cgtgatataa
     2461 cctgttcgtt ctcccgccag gaatgccacc gcttccgcca cctcttccgg gttaccgaaa
     2521 cggcccatcg ggatgagcgg tttgtgctgg ttttcgtcta gatctttcgt catatcggtc
     2581 cggataaatc ccggggccac ggcattgacg gtaatgttct ttttggccac ttcctgggcc
     2641 agcgctttgg tggcgccgat cacgcccgcc ttggccgccg agtagttcac ctggcccggc
     2701 agccctttta tccccgaaag ggaaaccaca ttgataatcc ggccccactt attcaccatc
     2761 atgcttttaa tgagtttccg ggtgatgaaa aacaggcttc caaggttggt attcatcacg
     2821 ctgttccatt cctcatcgtt catgaatacc agcaggttgt cttttctgat cccggcgtta
     2881 ttgaccagga tggcgatata atcgtcagga tgcgcctcca tccataaatc gagggctttt
     2941 tcggctgctt cctgcgaact cacatcgaac gggaggagct ccgcctcgcc gccgttaccg
     3001 acgacggcgg ccattgtttg ccgcgcttct tcctcattcg acctgtagtt gatgataaca
     3061 ggatatccca tctctgccag cctgatgcag gttgcttttc cgattccacg ggatccgccg
     3121 gtcaccagcg cgtatttctt catccgtcag atatttaagc tgttattgct gatatactcg
     3181 ttgatcttcc ggagatcggg cgacatgtag tggtcgtcgg caaaggccgg cacccgggcc
     3241 ctgagagcgt cgtagattcc gccggtaaac gtcgacatcc ggttacggat tttcaggtga
     3301 tcgaccgcct gcatcaacgt caggagatgg atcgacatca cctcgaaact gttgcggatc
     3361 acgcggccgg tgagaagagc cgcgtttgtt cccatgctca cgatatcctg gttgtcgttg
     3421 ttggtcggga tactgtggat ataaacggga ttggagagcg actggttttc ggcaacggtg
     3481 gaagtcgctg tgaactgggt tccctgcatg ccgagattaa ggcccagcgt gccgaggttg
     3541 acgaatggcg gaagctttcc gttgagtttg tcgttcatca ggaaattgag ctgccgctcg
     3601 gccagcatgg aaagtttggt tatggccagt ttgagtttat ccatctcgag tgagatgtaa
     3661 tcgccgtgaa aattcccgcc gtgaaaaaca ttaccgttcc ggtgatcgat gacagggttg
     3721 tcgttcacag agttgatttc gttgaggacg actctttcgg ctgcatggat cgtatcggca
     3781 accgggccga ggatctgtgt gatgcacctg agggagtaat attcctggat ttttttagtg
     3841 aagaccgttt cgccgtttgt cccgttgaag agatcttcgg cccttttgcg ggttaaacgg
     3901 ctgtcggcca aaatattctg gagcatgccg gtgatctcct gctgccccgg gtgaagctta
     3961 acgcggttga gttccagcga aaaatggtcg tcgtagcttt ccacgacttc gttgatcagt
     4021 gcagaggccg ccactgccca ttggagcagg cgtttggcgc ggatcacatt gacgatcccg
     4081 atgcccgaca ttaccgaagt cccgttgatc agggaaagcg cttcgcgtat atgcatcttc
     4141 accggttcca gtcctgcttt ccgcagggcc tcttccgttg ccatccgggt acctctcacc
     4201 gtcacttcgc cttcgccgat gagggccagg gccaggtggg ccagttgtac caggtcgccg
     4261 ctggcgccta cgccgccgtg gcgcgggatg accggggtaa tatcgtggtt gatgaaatcg
     4321 cgcagcagtt caacgaccga cggatggatg cccgagtagc ctttcatcag ggtattgagc
     4381 cgggccacca tcagcgcttt cacgtggatt tcgctgatcg gttcgcccat tccggcggca
     4441 tggctgcgga tgaggttata ctgcaatgcc tgccggtctt tttcctgcac tttatattgc
     4501 gacatcgggc ccaggccggt attgatgccg tagataattt tatttttcga gaaatccttt
     4561 aaaaacagaa aactgtcgtt gaccgcggaa aacgcttccg ggtcaaggct gacagtctcc
     4621 tggccgaaaa tgatctgctg aaaatcctcc aggcagatat cccttccacc aataacgatc
     4681 attattctga aattttccgc aaagtaagta aaaaaaagtc attagtcatt cgtcatcagt
     4741 cgtcagtcga aagtcaatga aaaatgaaaa acgcaaaatg caaaacggaa caggcatcag
     4801 tctcttcttc ccaggaaaat cataatgtag tagagcaatg ttgcaagtga tcccagggcg
     4861 gcgatgacat aggtataggc ggcccagcgc agggcatcct gtgctttgtc gtggttctgt
     4921 acattggtga tgccgctggt gttgagccat accagcgccc tgttggtcgc gttgatctcg
     4981 acgggcaatg tgataaagct gaaaagggtg gtgagggcaa aaaggaagat gcccagcagg
     5041 agaagttgcg ggaatacctc gatgaggaga acgccggcca gcagcaccca ctgcacccag
     5101 ttagaggcga aactgaccac cgggaccagt ttggagcgca tggtcagcca gttgtaggct
     5161 gtagcatgct gcacggcgtg gccgcattca tgcgcggcca cagcagcggc ggcaacattg
     5221 ctgctggcat agacgccttc actgagattt acggttttgg tgagcggatt gtaatgatcg
     5281 ctcagcatac ccggagtcga aaccacctta acgtcggaaa taccgttatc gcggagcatt
     5341 ttctctgcga cctctttacc ggtaaggttc tgatttatag gaaccgtcga atatttcttg
     5401 aatttggatt tgagctgcat ggagatcagc cagctcagga gcataaaaac gccgaaaatg
     5461 atcagcatgg gaatcgatat catagtttta aaattttaaa tgaaacaaga aaccatcaat
     5521 tttgttgcca gatttattca gattgtcttt cagacaagtt ggcagtatga tatcttgcaa
     5581 gaatcacctc acctttataa tactcctgtt tgaaacgcct tcaccattgg ccgaaagcct
     5641 taccaggtaa atccctgccg gataaggact cagatcaatt cttgccattt cctctccggg
     5701 atgcaaatct ttcttactca atatcaatcc tgagctatta attaattcaa tggagcaata
     5761 ttcacgatct tcatttgtct caacccaaac ccactcccgt gccggattcg gaaaaaccct
     5821 catttctctt tgatctttga actttaaact ttgaactgcc aagggcaatt gatattcaaa
     5881 cgctcccata tcaatccggt cgttggcata ccttggatta ttgttaaggt caagctccgg
     5941 caggccaagc ccggtcgtat ccggcgttcc ggcttcgata cagggtgatt gccaatcaag
     6001 atcataaatt ccgggaagaa acaggggatc aaggacgatg tttcctgtgc cccagctata
     6061 acctgcattt ccggtcagtt caatcccttc ctgagcgtct ttcagatcgg aatatgcgat
     6121 aaagagtgca acaagggaca tgatggcatc aagacggacc agggaagaag tgtcgggagt
     6181 ctcatcctgc caggcaatgc agttcaacat gaccgcgaca tggttatgtg cgtatagcac
     6241 agagccgtca gggctttcgt tgttgtggaa cgtattattg aaaagtgttg tttcaattgg
     6301 atttccggac gagaggaata ttgcgccgcc gctaaccggg gctgaatttt cgacaaaaag
     6361 gcaattaata atttcgttcg gggaaaagga tgcaatagca ccacccgaag tgttgctggt
     6421 attttctgaa aacgtacaat tctcaaatcg gggatcagcg ccaagtttca cactgacggc
     6481 gcctcctgaa tataagctgc tgccgcccag cgccccggca tttgcaagga atgtacattg
     6541 gctcagaacc gggcttgcat cattgatata tatccctcct ccggagccaa tcgaagaatt
     6601 gccggtaaac gtacaatttt caagtacggg cgcactgccg ttgtaaatca ccattccccc
     6661 gccggcgccg ttagcgatgt tattgttaaa agagcagttt ttaatgatgg catttgaagc
     6721 atccagggca atgccggctc cgtcgagggc tgagttattc tcgattagca ggttttcaaa
     6781 tacaggagct gaatcaacgc atctgatcgc acctccctgg aatgaagcgc aattccgtat
     6841 ggtgaagccg gatatcctga ccctgtttga gttatttaaa gtcaggccgc tgcccccgag
     6901 ggagttattg attgatccat ttgcaaggat ggaggaatcg gttaaactgg agtcggtggc
     6961 attgaaatag atcccgcgcc attctatggt atcattttca ccgtcaaaaa tgacaggttg
     7021 ggttggagtc cctgaaacca gcaattgacc aaggacttcc agccggaatg tgcccctgaa
     7081 aaggatctcc gttcctaatc ctatttccag tgtggaatcg ggttggacca acaggtttcc
     7141 atcgatcagg tagggggaac ccgaaatatc ccagttgcca ctcacatacc cgccggggat
     7201 gatggtctgg gcatgtatgt ttaagcagtt gaaaatgaac aaataccaaa tgatgcagcc
     7261 ggacaggttt ttcattcttg ctatttgtga aataagacag caattaaatc agaatagttg
     7321 caaaggatga aaaaagaaga ggctgctcag aaatatgaga cagcccctta cagcagaatt
     7381 aacctactta actcaccgca tatcaaacaa attccaaatt ccaagcacca aaagctaaac
     7441 aaattccaaa tgccaagcac caaaagccaa acaaattcca aatctccaaa tcttcaaatc
     7501 ttcaaatctt caaatcttca aatcctcaaa tctccaaatc tccaaatcta tcggtctgcc
     7561 ttcagcgctt cgatctcctt cctcagcgct tcgatctgag cttgttgttc tttcacggcg
     7621 ttgatgagcg ggatcacgaa ttcgccgtaa gagaggttca gggttttcat ttcgtcgtca
     7681 tccaccacaa gcccgctgaa ttctacgccc agttcatcca gggcctgttg gacatcctgt
     7741 gcgatcagcc catctcttct tcttttattt tcatcatcaa tataattgta agaagcagtt
     7801 ctcatcttca taacaaaatc aagccccagg tcatctttat acgaaatatt ctctttgagc
     7861 cttgcatcgg aataattggt ccatgttccg taacctccta ccgttgtagc gctggagtta
     7921 cctaaaacga tcttattgct ggcattcacc gatgccccgt agccgatggc ggtggcattg
     7981 gtcaggttat tgaacgctac atcagcctcg tatccgatcg ccgtgttgtt cgagccaaca
     8041 gtattgccat ccagggcatt gcgaccgagt gctacattgt tgttgccgga agtgttgcca
     8101 aacagggcct ggtcgcccac cgccgtgttg gatgtgccgg tgtttgatgc gggagtaccg
     8161 cttccataca aggccttata acctatggct gtattgtaag catcaccgct cgtgggcgta
     8221 tcatccgcgt taaacatggc ctcgatgccc accacggtat tccttctttt cccgacattg
     8281 ctataaagcg accggtagcc gacggccagg ttcgacccgc cggtagtcgt agcgtacata
     8341 gcctgataac ccacggctgt cgaatatgat cctccgttat tgcttaccat cgacatattt
     8401 ccgaccgctg tattgtagtt tcctgcatca ttgaactgca atgcaaaggc gccgcagccg
     8461 gtattgaagc tgccggatgc gttcaggctg agggcctggt cgccaacggc ggtattttcc
     8521 tggccggtat tggctgaagg agtgctgctg ccgctgagtg cctggaaacc gacggcggta
     8581 ttgtaggtag atctttcact cgtggtacgg ttatcggcgt tgtacatggc catataacca
     8641 acagcagtgc tgcggtcatt ttctttattg ttgtaaagcg cgccgaatcc catggccgta
     8701 ttttgctctc cctgagtatt ctggtataaa gcctgcgcac cgacacttgt attatggttt
     8761 cccatactgt tggtccagag agcagcatcc cctatagcgg tattgtactg cccgctgttg
     8821 gaagcggcgc tggtgctgcc gcgtaaagct tcataaccca acgcggtatt gaaggtttcc
     8881 ctgaccgatg cgttctggtt a
//
LOCUS       DAOPNH010000134         8889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8662_length_8889_cov_6.648404, whole genome shotgun sequence.
ACCESSION   DAOPNH010000134 DAOPNH010000000
VERSION     DAOPNH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_8889_cov_6.648404"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKZ74_08270"
     CDS             <1..547
                     /locus_tag="PKZ74_08270"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPM92792.1"
                     /translation="HRHLNIETLHAIMPVNIRERCESDRSLNSLRKALFGKELSYRYP
                     AEPFFRKGSARAQLVGGNLSILYSLTNTRSDISTNGKILFIEDLDEYLYHIDRMMLNL
                     RRSGKLEGLAGLIVGALSKMHDNEVSFQKSASDIIAEAVEDYPYPVCFNFPAGHGPDN
                     RALILGREVGLEINDEVRVIF"
     gene            656..1027
                     /locus_tag="PKZ74_08275"
     CDS             656..1027
                     /locus_tag="PKZ74_08275"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPM92793.1"
                     /translation="MSEASELGKKGEEIATRHLLKLGYIILDVNWRAGRNEIDLVARD
                     KEFLVVVEVKSRSSDSFAEPEDAVTRDKQQALIRAANAYVYKKNITLEVRFDIISIIF
                     NKEEARVNHMKDAFYPRLRTG"
     gene            1205..2356
                     /locus_tag="PKZ74_08280"
     CDS             1205..2356
                     /locus_tag="PKZ74_08280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPM92794.1"
                     /translation="MSQEKTPRQPRKPFQNAGKKETKTYRENKRGGRAEGRMEKPFRP
                     RPAEERGKKSLVKKDKPYAAREDGDKPPKKVFRKERPSTVGGPFEKRPKKSYRDEKSS
                     EKFERAERPAKKIIRRKTEGEPGLPQRKFATRSGVRIKATKAEPAGIRLNKYIANAGI
                     CSRREADDLIRAGVIKVNGEVVTTLGAKVMPGDKVQYADQTLSNERKQYLLLNKQKGY
                     VTTTKDPHAKNTVMELIAGACKERIYPVGRLDRNTTGLLLFTNDGELAKKLMHPASRI
                     LKMYHVVLDQPLSKQDLMKVAGGIEIDGDRIQVDGIEYVEDAKDRREVGMEIHSGQNR
                     IVRRIFESLGYKVTKLDRVMYAGLTKKNLPRGKWRFLSEQEVINLKKMR"
     gene            2369..2860
                     /locus_tag="PKZ74_08285"
     CDS             2369..2860
                     /locus_tag="PKZ74_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPM92795.1"
                     /translation="MRGDPKLYRILEELGITFDYYEHPAAPTIEEALKNWKNIDSTHC
                     KNIFFRNHKGNRHYLVIMEHSRPLAIHDLEKKLKQGKLTFASPERLMKYLGVTPGSVS
                     LFGLINDEADHVYVFLDEVLREAKNLSFHPNDNTASLVITGEGFHKFLEWSGNGFEFL
                     ALA"
     gene            complement(2958..5072)
                     /locus_tag="PKZ74_08290"
     CDS             complement(2958..5072)
                     /locus_tag="PKZ74_08290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPM92796.1"
                     /translation="MRTLFGISLLLMGIITSCTTGEKKMKTAENPFFAPYGTPFETAA
                     FDRIKNEHFLPAFHEGIRVHDEEIAAIVANPAAPDFENTIVALDRSGDLLAGVSGVFY
                     NLTSANTNDELQSIAKEISPVLTGHYDNILLNAGLFKRIKAVYEQRDNLNLDTEQAML
                     LEKTYKQFARGGSNLPDDKQATLREINKELSMLSLQFGDNLLAETNAFRLVIDNESDL
                     EGLPAFVRDAAASAAREDSLDGKWLFTLHSPSRIPFLQFSAKRELREKVLKAYANRGN
                     NNNQYDNKEIAAKMASLRVKKANLLGYATHADYILEENMAKNPAAVYDFLDKVWSAAL
                     PVAKKEAAELQAMIDAEGGNFKLEAWDWWYYAEKLRKQKYDLDEEALKPYFSLENVRL
                     GMFDVATRLFGITFTERNDIQKYHPEVKVFEVKDTDGSHIGILYMDFFPRASKEGGAW
                     MNNYRDQYMAGDSNVSPVITTVCNFTKPAGDVPSLLTFEEVETLFHEFGHALHGLLSE
                     CRYRKLSGTSTPRDFVELPSQIMENWAGDPEVMKLYARHYQTGEVIPQELIDKITASG
                     HFNQGFATVEYMAACYLDMDIHTLKDSSLMDALGFESASMSKIGMMPEIIVRYRVPYF
                     AHIFAGGYSSGYYSYLWAEVLDADAFQAFKETGIFDPATAKAFRDNILSRGGTDDPMK
                     LYKQFRGKEPDVAPMLKRKGLI"
     gene            complement(5141..5395)
                     /locus_tag="PKZ74_08295"
     CDS             complement(5141..5395)
                     /locus_tag="PKZ74_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92797.1"
                     /translation="MKLYDVISYLGYLSGIFGGLMMLAGVIGFLTGTEFLGVKYYYNY
                     FFYANSFLLFGVFIILGTRQFCHCHCHTHDDACQHEDHQK"
     gene            5509..6087
                     /locus_tag="PKZ74_08300"
     CDS             5509..6087
                     /locus_tag="PKZ74_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM92798.1"
                     /translation="MTKDQTRDKILAVAAKMFGKYGFQKTTVDEIARTAHKAKGSVYY
                     YYKSKEDLFLAVVMQEIQFLKTGLIRVIVDSQDATGMIRNYLMNRMMLMKDAVNYHES
                     LKADFVADFSFLNDCREDFTRFEIDLMKAIIDRGIRENRFQIKDSYATAQVIILAMKA
                     IEIPFYHHHKISEYEQTIVELVDILVRGLEKP"
     gene            6339..8585
                     /locus_tag="PKZ74_08305"
     CDS             6339..8585
                     /locus_tag="PKZ74_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM92799.1"
                     /translation="MKTIAYSIIRYRKAIIILTLIITGIMAYFFRYLKVDPDVFNYLP
                     DDDPKAMLFREIGNKYGGNYTGIIGLETKNIFNTETLENIRRITDSVKIIPGVATVTS
                     LTNIIDIKGSDWGIEIGNLVDKYDIPDTQEELAALRTYTLSQEMYRGAIVSEDATVTA
                     VMVKIAEGVDKIKVASAIREKVESLNLSEKVYFGGMPFAFKSLADIIMGDIVILAPIT
                     ALVIILVLLLSFRSWRGVVMPLVTVVISVIWTIGLMGLFRVEISIISDVTPVILLAVG
                     SAYTIHVINRIRQEKAENPERPMQEALAYIIIPVFLAAATTMAGFISFIFGSYLTMIS
                     TFGIFTALGVFFALILSLTFAPALLYAFPEKKPALPGKTSDRSDLIGMILAKIQRRVI
                     RHPKRVILTWGIILAGAIAGIFMIERNVNILDYFRKSDPTHQSEILFKNKFGGSMPVY
                     ITVKGDVQDPEMLRIMADIGDYLKNDGYVSHAQSVANLIEEMNEVMGEGKTIPDEKTK
                     VEQLWFLLEGQDIMEQLVTYEKDEGLVNTTTGKGDLGEMTGLTENLQAYIERHPDWKD
                     KVEFTGMPSLYLQIDRSIVNSQMQSLLYAIVFVFLLVTLILRSLRLGLHALIPIIATL
                     LVLFGFMGLIGLPLDIATVLVGSVSIGIGVDYAIHMITHYNHEKKHGRDSKRALGHAI
                     RISGRAIVINVLSVSLGFLVLTFSNLVPLQHFGLLVGVTMIISGAAALTLLPATILFR
                     EKGIENSE"
     gene            8603..>8889
                     /locus_tag="PKZ74_08310"
     CDS             8603..>8889
                     /locus_tag="PKZ74_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92800.1"
                     /translation="MKNVVVIMILGLMVSPVFSQTASEVLRKADDVMYAPKDQKAAVK
                     IILTDKKSNTQERAAQYIQKGSEMRLFKFTAPASQQGIAFLSLPDDVMYLYL"
BASE COUNT         2433 a         2119 c         2196 g         2141 t
ORIGIN      
        1 ccaccgccat cttaacattg aaacgctgca cgctatcatg cccgtgaata tccgcgaaag
       61 gtgcgaaagc gaccgctcgc tcaacagcct gagaaaagcc ctgttcggaa aggagctatc
      121 ataccgctac cccgccgaac cctttttccg caaaggatca gccagggcgc aactggtcgg
      181 tggcaacctg tcgatcctct acagccttac caatacccgt tccgatatca gtaccaatgg
      241 caaaatcctg tttattgaag accttgacga gtatctctac cacattgacc ggatgatgct
      301 gaacctgcgc cgttcgggca aactggaggg gctggcgggg ctgatcgtcg gggcattgag
      361 caaaatgcac gacaatgagg tctcctttca gaaatcggcc agtgatatca tcgccgaagc
      421 ggtggaagac tacccctacc ctgtttgctt caatttcccc gccgggcatg gcccggataa
      481 ccgtgccctc atcctcggaa gagaggtcgg actggagata aacgacgaag tcagggtgat
      541 tttttagtta ctgatatcca gcttcttatc tcatattttt aaaaatattt tacaccgacc
      601 gggtattttt tggtgaaaat ccggattaat gattagtcgt tggtcgaaca aatttatgtc
      661 agaagcatca gaactcggaa agaagggcga agagatcgcc accaggcacc tcctgaaact
      721 gggatacatc atcctggacg tcaactggag ggcgggacgc aatgagatag acctcgtggc
      781 gcgggataag gaatttcttg ttgttgtgga ggtgaaatcg cgaagcagcg attcgtttgc
      841 agagccggag gatgctgtga cgcgcgacaa gcagcaggcg cttatccgtg cggcgaacgc
      901 ttatgtctat aaaaagaaca tcaccctgga ggttcgtttc gacatcatct ccatcatatt
      961 caataaagaa gaagcccgcg tcaatcacat gaaagacgct ttttatccaa gattgaggac
     1021 aggatgatgg aattgttgtt acctttgcga aataataaat tccaaattcc aaactccaaa
     1081 ttccaaacaa atacgaaaaa ttaaatccaa aatccaaaac cattttgaat ttgctttttt
     1141 ggatttgttt ggtgcttgga atttggaatt tggaatttta gttcaatgca atgaataatc
     1201 tgttatgagt caggaaaaaa ctccccgcca gccgagaaag ccgtttcaga atgccggaaa
     1261 gaaagagaca aaaacctacc gcgagaataa gcggggcgga cgcgccgaag gcaggatgga
     1321 aaaaccattt cgtccaaggc cggctgaaga gagaggaaaa aaatctcttg taaagaaaga
     1381 caaaccatac gctgcaagag aagacgggga taaacctccg aaaaaggttt tcagaaaaga
     1441 acggccttcg accgttggag gtccgttcga gaaacgccca aagaaatcct atcgggatga
     1501 aaaatcttct gaaaaattcg agcgagcgga aagacccgca aaaaagatca tccggagaaa
     1561 gacagagggt gagccgggtt tgccgcaacg taaattcgcc acccggagcg gagtaaggat
     1621 caaagccacc aaggctgaac cggccgggat caggctgaac aagtatatcg ccaatgccgg
     1681 tatctgttcc aggcgcgagg cggatgacct gatcagggca ggcgttataa aagtaaacgg
     1741 agaagtagtc accacattgg gggccaaagt gatgcccggc gacaaagtgc agtatgccga
     1801 ccagacgctg tcgaatgaaa gaaagcagta cctgttgctg aacaaacaga agggctatgt
     1861 gaccacgacg aaagacccgc acgcaaagaa cacggtcatg gagctgattg ccggggcctg
     1921 caaagagcgc atctaccctg tcggccggct cgaccgcaac acgaccggcc tgttgctgtt
     1981 caccaacgac ggcgaactgg cgaaaaagct gatgcacccg gcctcacgga tcctcaagat
     2041 gtaccatgtg gtgctggacc agccactatc gaagcaggac ctgatgaagg tggccggtgg
     2101 catcgagatc gacggggacc ggatacaggt cgacgggatc gaatatgtgg aagacgccaa
     2161 agaccggcgt gaagtcggca tggagatcca ttcggggcaa aaccggatcg tccggcggat
     2221 cttcgaatcg ctgggttata aggtgacgaa gctcgaccgg gtgatgtacg cagggctcac
     2281 caagaaaaac cttccccgcg gcaaatggag gttcctctcg gaacaggagg tgatcaacct
     2341 gaaaaagatg cgttaaaatc ttaaagctat gcgcggagat cccaaattat accgaatcct
     2401 ggaagaactg ggcattacgt tcgattacta tgagcatccc gcggctccga ccatcgaaga
     2461 ggcgctgaaa aactggaaga atatcgactc gacgcactgc aagaatatct ttttccgcaa
     2521 ccacaaagga aaccggcatt acctggtgat catggagcac tcgcggccgc tggccatcca
     2581 cgatctcgaa aagaaactga agcagggaaa actcaccttt gcttcacccg aacggctgat
     2641 gaaatacctt ggcgttacgc cgggttctgt ttccttattc ggtctgataa acgatgaggc
     2701 cgatcatgtt tatgttttcc ttgatgaggt tttgagagag gcgaaaaatc ttagctttca
     2761 cccgaacgac aacaccgcca gcctggtgat caccggcgaa ggattccaca aattcctgga
     2821 gtggtccggg aatgggtttg agtttcttgc attagcatga gatggcatct caaaaccatc
     2881 tctatctact cctaactaac agtataacag tgcactatgc aatagttctg ccgtaaatga
     2941 gatgccatct catttcgtca aatcagtcct ttccttttca gcatcggcgc aacatccggc
     3001 tctttgccac ggaactgctt gtacagcttc atcgggtcgt cggtgccgcc gcgggaaagg
     3061 atgttatcgc ggaacgcttt cgctgtcgcc ggatcgaaga tgcccgtctc tttgaatgcc
     3121 tggaacgcgt cggcatccaa aacttcggcc cacaggtagc tgtaatagcc ggaagaataa
     3181 ccgccggcaa agatgtgggc gaaatagggc acacggtagc ggacgatgat ctcgggcatc
     3241 atgccgatct tcgacatgga agcgctctca aagccaaggg catccataag agaggaatct
     3301 ttcagggtat ggatatccat atcgagataa caggcggcca tatattccac tgttgcaaag
     3361 ccctggttaa agtgaccgct ggcggtgatc ttgtcgatca actcctgggg gataacttcg
     3421 ccggtttggt aatgacgggc gtagagcttc atcacctccg gatcgccggc ccagttctcc
     3481 atgatctgtg aggggagctc aacgaaatcg cgtggcgttg aagtgcccga aagcttgcgg
     3541 tacctgcatt ccgaaagcag gccatgcagc gcgtgcccga actcatggaa aagggtttcc
     3601 acctcttcaa atgtcagcag cgacggaaca tcgccggcag gcttggtaaa attgcaaaca
     3661 gtggttatga ccggggagac attagaatcg cctgccatgt actgatcgcg gtagttgttc
     3721 atccatgcgc ctccttcctt gctggcgcgc gggaagaaat ccatataaag gataccgata
     3781 tgtgacccgt cggtgtcttt gacttcaaag acctttactt caggatgata cttctggatg
     3841 tcgtttcttt cggtaaatgt gatgccgaaa aggcgtgtgg ccacgtcgaa catgccaagc
     3901 ctgacatttt caagcgaaaa ataaggcttc agcgcttctt catccaggtc atacttctgt
     3961 ttgcgcagtt tttcggcata ataccaccaa tcccaggcct cgagtttgaa attcccgcct
     4021 tcggcgtcga tcatcgcctg cagttcggcc gcttcctttt tagctacagg gagggcagcc
     4081 gaccatactt tatccaggaa atcgtaaacg gcagccggat ttttggccat attctcttcc
     4141 aggatataat cggcatgggt ggcatagccc agcaggttag cctttttcac ccggagtgaa
     4201 gccattttgg ctgctatttc cttattatcg tactgattat tgttgtttcc ccggttggca
     4261 taagctttca gcactttttc ccgcaattcc cgtttagcgg aaaactgcag gaaagggata
     4321 cgcgaagggg aatgcagggt aaacagccat tttccgtcga gactgtcttc tctggcggca
     4381 gaggcggcgg catcccttac aaatgcagga aggccctcca gatcggattc attatcgatc
     4441 accagccgga aggcattggt ttcggccagc aggttatcgc caaactgcag cgacagcatg
     4501 gaaagctctt tgttgatctc tctcagggtc gcctgcttgt cgtcaggcag gttggaacca
     4561 ccgcgggcaa actgtttgta ggtcttttcg agcagcatcg cctgttcggt atccaggttc
     4621 aggttatctc tctgttcata aaccgctttg atccttttaa acaacccggc attcaacagg
     4681 atattatcgt aatgcccggt caggacggga gatatttctt tggcaatcga ctgcagttca
     4741 tcattggtat tagccgatgt aaggttatag aaaaccccgc tgacgccggc cagcaggtcg
     4801 ccgctgcggt cgagcgctac gatcgtattc tcaaagtcgg gtgcagccgg attggccaca
     4861 atagcggcaa tttcttcatc atgaacacgg ataccttcat ggaaagccgg caggaagtgt
     4921 tcattcttga tccggtcgaa tgccgcagtt tcgaaagggg tgccataagg ggcaaagaag
     4981 gggttttctg ctgttttcat ctttttttca cctgtggtgc atgatgtaat gatccccatc
     5041 aacaacaggg atataccaaa taatgttctc atttcaacct ggtttttaat ttacccggcg
     5101 aaattaggaa gaaaacagaa gtataaagcc ttattccggt ttatttttga tgatcttcat
     5161 gctggcacgc atcatcatgg gtgtgacaat gacagtggca aaactggcgg gttcctaaaa
     5221 tgatgaatac tccgaataag aggaagctgt tggcatagaa gaagtaattg taataatact
     5281 tcacacccag gaactcggtt ccggtcaaaa aaccaataac gcctgccagc atcatcaatc
     5341 cgccgaaaat acctgacagg taacccaggt agctgataac atcataaagt ttcataaata
     5401 tctgttttgg tgaaaatcaa agttaaatat tttattcacc cggttgtttt gatattgatt
     5461 tttttatatt tttaaacctt taaacctata tagtcaaata gtttaaaaat gactaaagac
     5521 cagaccaggg acaagattct ggcagtagct gccaaaatgt tcggaaaata cggattccag
     5581 aaaacgactg tcgacgaaat agcccgaacg gctcataaag caaaaggatc agtttactac
     5641 tattacaaga gcaaggaaga tctgttcctg gccgttgtca tgcaggaaat ccagtttctt
     5701 aaaacaggcc tgatccgggt gatagtcgac agccaggatg cgacaggaat gatacgcaat
     5761 tacctgatga acagaatgat gcttatgaag gatgcggtga actaccacga atcgctgaaa
     5821 gccgattttg tggccgattt cagcttcctg aacgattgcc gcgaagattt cacccggttt
     5881 gagatcgacc tgatgaaagc catcattgac aggggaatca gggagaacag gttccagatc
     5941 aaggattcat atgccacagc gcaggtcatc atcctggcca tgaaagccat cgaaatcccg
     6001 ttctaccatc atcataaaat ctccgaatac gagcaaacca tagtcgaact ggttgatatt
     6061 ctggtcagag gactggaaaa accctgatat ttatattcct ttaacaattt ttaatgaaat
     6121 tcaggatttt tatgcttata tttatactat atttgactat aaaacgtttt tggtcatata
     6181 gattaatttt cttcgccggg ataaatcaag atcaaatttg aaattggaaa ctcgtaaagt
     6241 caatgttcat tataataaac ttgataatta aaagcaacag atttattttt tttattcata
     6301 atctgactat aaaacgatat tagtcattta gtatattaat gaagacaatt gcatatagta
     6361 ttatccggta cagaaaggcc atcataatcc tgactctcat catcacaggt ataatggctt
     6421 actttttccg atacctcaaa gtggatcccg atgttttcaa ctacctcccc gatgatgacc
     6481 ctaaagcaat gcttttcagg gagataggaa acaaatacgg gggtaattac accgggatca
     6541 tcggcctgga gaccaaaaac atcttcaata cggagaccct tgaaaacatc cgtaggataa
     6601 ccgattcggt gaagatcatc ccgggagtgg cgacggttac cagcctcacc aacatcatcg
     6661 atatcaaagg gagcgactgg gggattgaga tcggcaacct ggtcgacaaa tacgacattc
     6721 ccgatacgca agaagagctg gcagccctga gaacctacac tttatcccag gagatgtacc
     6781 ggggggccat tgtttcggag gatgccaccg tgacagccgt catggtgaaa attgccgaag
     6841 gagtggataa aattaaagtc gcatcggcta tccgggagaa ggtcgaatcg ttaaaccttt
     6901 ccgaaaaagt ctatttcggc ggtatgcctt ttgcgtttaa aagcctggcg gatatcatca
     6961 tgggcgatat agttatcctg gcgccaatca ccgcactggt catcatcctg gtcctgcttc
     7021 tatcattccg ctcatggcgc ggcgtagtca tgccactggt tacggtcgtc atcagcgtca
     7081 tatggaccat tggcctgatg ggcctgttcc gtgttgaaat ctccattatc tcagatgtta
     7141 ccccggtgat tctgctggcc gttggcagcg cttataccat ccatgtcatc aaccggatac
     7201 ggcaggaaaa ggcagagaat cccgaacggc ctatgcagga ggcgctggct tacatcatta
     7261 tacccgtttt cctggccgcc gccaccacca tggccgggtt tatctccttc attttcggct
     7321 cttatctgac gatgatcagt actttcggta tttttaccgc actgggtgtt ttctttgccc
     7381 tgatactttc gctgactttc gcccctgccc tgctgtacgc tttcccggaa aaaaaacctg
     7441 ctttaccagg aaaaacaagc gaccggtcgg acctgatcgg aatgatcctg gctaaaatcc
     7501 agcgacgggt cattcgacat ccgaaaaggg tgatcctgac ctgggggatc atcctggccg
     7561 gcgccattgc cggcatcttt atgatagagc gaaatgtgaa catcctcgat tatttccgga
     7621 aatcggaccc cacccatcaa tccgaaatcc tgttcaagaa caaattcggc gggagcatgc
     7681 cagtatacat cacggttaaa ggcgacgtac aggacccgga aatgctccgg atcatggccg
     7741 atatcgggga ttaccttaaa aatgacggat atgtgtcgca cgcccagtcg gtggctaacc
     7801 tgatcgaaga aatgaacgaa gtgatgggag aaggaaagac gatccccgat gaaaaaacca
     7861 aggtagaaca gctctggttc ctgctggaag gacaggatat catggaacag ctggtgactt
     7921 atgaaaaaga cgaagggctt gtcaacacta ccaccggcaa aggcgacctc ggcgaaatga
     7981 ccggcctcac tgaaaacctg caagcttata ttgaacgcca tccggattgg aaagacaagg
     8041 tagaattcac agggatgcca tcgctttacc ttcagattga ccggagcatt gtcaatagcc
     8101 agatgcaatc attactatat gccatcgtat ttgtatttct ccttgtaaca ctgatcctga
     8161 gatcattacg gctaggatta catgccctga tccccattat cgccactttg ctggtcctgt
     8221 tcggattcat gggcctgatc ggacttcccc tcgacatcgc cacggtcctc gttggcagtg
     8281 tttccatcgg gatcggggta gattacgcca tccacatgat cacccattac aatcatgaaa
     8341 agaaacacgg gagggattca aaaagagcgc tcggacatgc catccgcatc agcggaaggg
     8401 ccattgtcat taacgttctt tcggtttcgc tggggttcct ggtcctgact ttctcaaacc
     8461 tggtcccatt gcagcacttc ggcctgctgg taggggtaac gatgatcatt tccggcgcgg
     8521 cggcgctgac attgctgccg gctacgattc ttttcaggga aaagggaata gagaatagtg
     8581 aatagttaaa ttttaattga ttatgaagaa tgtggttgta attatgattt tagggttgat
     8641 ggtttcgccg gtgttctcac agacggcttc cgaagtgctg aggaaggccg atgatgtgat
     8701 gtacgctccg aaagaccaga aagcggcagt gaagatcatc ctgaccgata aaaaaagcaa
     8761 tacgcaggaa cgggccgctc agtatattca gaaaggctcc gaaatgaggt tgtttaaatt
     8821 caccgcaccg gcatcgcagc agggaatcgc cttcctgtcg ctgccagatg atgttatgta
     8881 cctgtatct
//
LOCUS       DAOPNH010000135         8885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8669_length_8885_cov_6.214043, whole genome shotgun sequence.
ACCESSION   DAOPNH010000135 DAOPNH010000000
VERSION     DAOPNH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8669_length_8885_cov_6.214043"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKZ74_08315"
     CDS             complement(<1..545)
                     /locus_tag="PKZ74_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPM92801.1"
                     /translation="MINPYYYHDMIRQLDIRPGSLVILAGDVTRLALAGKRMEIGFDI
                     HTFTGVVKQCLGKGGTLVIPAYNFNLRNHDYFHPKKSLPITGSLAVAALKRPEFIRTS
                     HPLHSFLVWGEQAGALAALSNSSSFDERSPFAFFRDHKALMLLVDTTIAEAFTFVHHV
                     EEMEKVKYRRYKKLIINVEGE"
     gene            complement(542..1027)
                     /locus_tag="PKZ74_08320"
     CDS             complement(542..1027)
                     /locus_tag="PKZ74_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM92802.1"
                     /translation="MEKIEFIRLDNAELPDWDEIPGLFNEMYARMDEMGLMVPLAADG
                     AQKWLRSTRNTAGKFGIVVLARIGEVPVGFAHGMIKFLPDYLGNHSVGLITHVFVKDI
                     ARKKGLGRELVKMLEGWFHDKNVHSIELQVINDNLPAQKFWENLGYRVELTQYRKIRS
                     K"
     gene            complement(1032..1286)
                     /locus_tag="PKZ74_08325"
     CDS             complement(1032..1286)
                     /locus_tag="PKZ74_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92803.1"
                     /translation="MMKEIEIRNQVMALIHDKLARLSVKESELGYPFDLVKSGLLNSL
                     EFVDLVASLEKENDREIDFEKELATGAFTTLGGLIKAFSS"
     gene            complement(1270..2262)
                     /locus_tag="PKZ74_08330"
     CDS             complement(1270..2262)
                     /locus_tag="PKZ74_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM92804.1"
                     /translation="MSYLDFMLETYDFERDDRFTQIFDLTFDLSVHDMLLAWSIGACL
                     VVPDDSSSFAMSRFIRDKKPTVWFSVPSVPALMNRMRLLKPDTFPSLRLSFFCGEALM
                     AGDARAWKFAAPNSSLINLYGPTEATIAISGYSLPGDETKWKTALGIVSVGKVFDGNK
                     YLILRENPSDARGELCLCGNQVVEKYLDNEEADKQSFFTDADDMERYYKTGDMVSVDE
                     QGDLFYLGRKDSEVKISGYRVNLKEVENVLLESETVTQAVVVYHDSSMSLLAFLVLSE
                     IPPGDALQKLTEYCQSRLPWYMVPGKFIFVKEIPLNDNGKIDRKQLLKKYDEGN"
     gene            complement(2187..2801)
                     /locus_tag="PKZ74_08335"
     CDS             complement(2187..2801)
                     /locus_tag="PKZ74_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM92805.1"
                     /translation="MTELQEDILASLGGRLWDTARRFPDRTALEQGDRKVTYRELFAA
                     ASRFAHAIADLENPAHFVAVMADKSPACYAGILGCMMAGKAYMPLNPRFPAARNLFML
                     RKSGARIIIAGENSDDELEQILENHLQEIYILFPEEVADYHHHDFVPRITEPSDPAYL
                     YSPQVLPANRKGYRSPVPMSFRTSISCSKRMILSVMTALPRSST"
     gene            complement(2798..3997)
                     /locus_tag="PKZ74_08340"
     CDS             complement(2798..3997)
                     /locus_tag="PKZ74_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HPM92806.1"
                     /translation="MLNSLRLYAKYFIVSAISPAGKYLWLDTGRYIFNKDIFKKEPHK
                     EPDLQAKALMKAIAWLVRSQQANTDGGMGSFHLINKWSSSYPETTGYIIPTLLNFGRK
                     YDNDEVTGSAMKAAKFLLKIQKKSGGWQGGRVAENKPEIVFNTGQAIRGMMAAYNFSG
                     EQKYLDAASAAGRWLSGIQNPEGYWKEFALMNQPRVYDTFVDAPLLELYGLTGEESLK
                     NAAIRNLDWVVGEKMLENGWFEDCDNTIRRNDKPILHTIAYTLDGLVDCGLMLEDEKY
                     IAAARKGAAQLREIFPERGYLHGRYDRNWHGSEHMICTGSAQISIVWLKFFRISGERV
                     WYDAAGQMLDLLMTVQSRMRGEPVDTLGAIPGSFPLWGRYEPFAFPNWATKFFADALL
                     LESELRP"
     gene            4136..5851
                     /locus_tag="PKZ74_08345"
     CDS             4136..5851
                     /locus_tag="PKZ74_08345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPM92807.1"
                     /translation="MDNFVVSARKYRPATFDMVVGQQSITSTLKNAIRNNQLAQAFLF
                     CGPRGVGKTTCARILAKTINCMNPRDKIEPCDQCESCLSFNRSASFNIHELDAASNNS
                     VDDIRNLVDQVRIPPQVGKYKVYIIDEVHMLSAAAFNAFLKTLEEPPPYAKFILATTE
                     KHKIIPTILSRCQIFDFRRIVVKDIADYLAYVAKSEGVDAEEEALHVIAQKADGAMRD
                     ALSFFDQLVSFSGSQLTYQTVTEHLNILDHAYYFRIMDQALSQDTSGILLTLDEIISR
                     GFDGQHFLIGLGEHLRNLLVCQDPKTVTLMDISSNLQQKYAEQAGKCSPAFLLQALEI
                     HNQYDQSYKTANNKRLQLELVMLLISAAAVKSGSPEPEKKISLIPPPADKKPGPEPGI
                     PLTPKPEENKTVAPPVAAVPKPSAALSASRTISIKPENHTPKKVGATGEETPAGPVNQ
                     KEFTQEKLEKVWLYYTESIAGQYPNFYSILSTRKPVLKENFLIYLQLDNKTQEITLKD
                     RRADLLDFLRNELGNQSVQMESVVIETATQSKPYTPDEKFRSMVEKNPELKVLKEGLD
                     LEIEF"
     gene            complement(5903..8755)
                     /locus_tag="PKZ74_08350"
     CDS             complement(5903..8755)
                     /locus_tag="PKZ74_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPM92808.1"
                     /translation="MKRIVFYLISSLLLLFAFSLPAQEISYAPGSVLPLDPDVIYGKL
                     DNGMTYYIRENREPKERAEFYLAVNVGALSEDADQNGLAHFTEHMAFNGTKNFSKKAI
                     LNYLQSIGMKFGPEINAFTSSDETNYMLQKVPTTDPKIVDTALLILYDWATNISFEDE
                     EIDAERGVIHEEWRTGRSAMFRMMREANKVIYKDSKYAVHDVIGDIDIIDNFSHDVIR
                     RFYSDWYRPDLQAIIAVGDFDGKAMEQHIKQLFGTIPVKNNPREREYFPVPNHDETLV
                     TILTDKEAQYPIVQIYYKHEPANEKNMEYYREQLKQQLFNSMLNARLQELILSENPPF
                     VYGYAFYTNIARTKDGFLSLAVGNNNELDKALRALLVENKRVKEFGFTTTEFDRAKSE
                     LKRGIEKQFAEKDKMASDQYVWQYFSHFLEQEPTPGVAYDFAFTNAMLPGITLEEVNA
                     QAGKWIIDKNRVVSLMAPDNPDIKVPSEQEVRDIITAVETEPITAYVDKVTDKPLMAA
                     EPTPAKVAKKGKDKTLGTTTWTFENGVKAVMKTTDFKDDEILMSAYSFGGSSLYETKD
                     LMSAMFASDISLESGLGEFDRIALDKKLTGVIASVRPVIKGSSEGLEGSCSVKDLETM
                     LQMVNLYFTAPRTDEVAFNAFIKRMKAMLDNKALEPESALQDTVTVLMANHHPRVRPL
                     TSAILDETNLKKARSIFKTRFGDPGSFTFYFVGNIDPVAAKPLIEKYLGGLPKVSREE
                     TFKDNNIRPPAGKVERVIEREMKVPKGTVYLTYTGTFDYDDFQSRLNLSALCDILDVR
                     FVETIREEQSGTYGAAVYESMDKYPYENYSVTIFFDCDPQNAGKLKQIVYDEIGKLKT
                     DGPTEKDLHGVKENKIKVHQENLRQNNYWLGIIKNKDYYGTGLDEYMKYEDYVNSMSV
                     GSLKTAAQQFFGDNIIEAVLMPANMEDNISNPVHPE"
BASE COUNT         2198 a         2250 c         2124 g         2313 t
ORIGIN      
        1 tcctcacctt ccacatttat tatcagcttc ttataacgcc tgtatttcac tttttccatc
       61 tcttccacat ggtgaacaaa cgtaaaggct tcggcgatgg ttgtatcgac gagcagcatc
      121 agcgccttgt gatcacggaa aaatgcaaac ggggagcgct catcgaagga gctgctgttc
      181 gaaagcgcgg ccagggcacc ggcctgctcg ccccaaacca ggaatgaatg gagcggatgg
      241 ctcgtccgga tgaattcagg gcgtttcagc gccgccaccg ccagtgaacc cgttatgggc
      301 aacgattttt tcggatggaa gtagtcgtgg ttgcgaaggt tgaaattata agccgggata
      361 accagtgtgc ctccctttcc gaggcattgc ttcacaacac cggtaaacgt atggatatcg
      421 aaaccgatct ccatccgttt gccggccagc gccagccggg tcacatcccc cgccaggatg
      481 accaaactac ccggacgtat gtccagttgc cggatcatat cgtggtagta gtaaggattt
      541 atcacttcga acggattttg cggtattgcg tcaattccac cctgtacccc aggttttccc
      601 agaacttctg tgcaggtaaa ttatcattaa ttacctgtag ttcaatggaa tgaacatttt
      661 tatcatgaaa ccagccttcc agcattttca ccaattctcg gcccagcccc tttttcctgg
      721 ctatatcttt gacaaaaacg tgcgtgatca atcccacgct atggtttcca aggtaatcgg
      781 gcaggaattt gatcatgccg tgggcaaagc caaccggaac ctctcctatc ctggccagga
      841 cgactatgcc gaacttcccc gccgtattcc gggtcgaccg cagccacttc tgcgctccgt
      901 cagcggccag cggcaccatc agccccatct catccatccg ggcgtacatc tcgttgaaaa
      961 gccctgggat ttcatcccaa tcgggaagtt ccgcattgtc gagccggatg aattcgatct
     1021 tttccatggc ttcaggatga aaatgctttg atcagtccgc ccagcgtggt aaaagctccg
     1081 gtcgcaagct ctttctcaaa atcgatctcc cggtcatttt ccttttccag ggaagcgacc
     1141 aggtcgacga attccagcga attaagcagg ccgcttttca ccaggtcgaa cggatacccc
     1201 agttcacttt cttttaccga caggcgggca agcttgtcat ggatgagcgc catcacctgg
     1261 ttccggatct caatttcctt catcatactt tttcagcagt tgtttccggt caattttacc
     1321 gttatcgttc agcgggattt ctttcacaaa gataaacttt cccggcacca tataccaggg
     1381 aaggcgtgac tggcaatatt ccgtcagttt ctgtaaggca tcaccaggcg ggatttctga
     1441 taataccagg aaagccagca gtgacatgga tgaatcatga tagaccacaa cggcctgtgt
     1501 aacagtttcg ctttccagga gcacattctc gacctctttc aggttaacgc ggtaaccgga
     1561 aatcttcacc tcgctgtctt ttctccccag gtagaagagg tcgccctgtt catcgacgct
     1621 caccatgtcg cccgttttat aatatctttc catgtcatcc gcgtcggtga aaaatgactg
     1681 tttgtcggct tcttcattat ccaggtactt ttcaacgacc tggtttccgc acaggcaaag
     1741 ttcaccacgg gcatcggacg ggttttcgcg caggatcaga tatttgttgc catcgaacac
     1801 tttgccgaca gagacgattc ccagggctgt tttccatttc gtttcatcac caggaaggct
     1861 atagccggag atagcaattg tagcctctgt agggccatag aggttgatga gggaggaatt
     1921 gggcgctgcg aatttccagg cccgggcatc accggccatc agcgcttcgc cgcagaaaaa
     1981 gctcaggcgc agcgaaggaa aagtgtccgg tttaagcaac ctcatccggt tcatcaacgc
     2041 cggcacggaa gggacggaaa accataccgt cggtttctta tcccggataa accgcgacat
     2101 ggcaaaggag gaactatcgt cggggacgac caggcaggcg ccaatcgacc atgccaggag
     2161 catatcgtgg acgctcaggt cgaatgtcag gtcgaagatc tgggtaaagc ggtcatcacg
     2221 ctcaaaatca tacgtttcga gcatgaaatc gaggtacgaa atgacattgg cacaggagac
     2281 cggtacccct ttcggttcgc cggtagtacc tgaggtgaat aaaggtaagc cggatccgaa
     2341 ggctcggtga tcctggggac aaagtcgtga tggtgataat cggctacttc ttcgggaaac
     2401 aggatataaa tctcctgcag gtggttttcg aggatttgtt ccaattcatc gtcggagttt
     2461 tctcctgcga taatgatccg ggcgcccgat ttccggagca taaagaggtt ccgggcggca
     2521 ggaaacctgg gattgagggg catataggct ttgccggcca tcatgcaacc gaggataccg
     2581 gcatagcagg ccgggctttt gtcggccata acggctacga agtgggccgg gttctccagg
     2641 tcggcgatgg catgggcgaa ccgggaggca gcggcaaaga gctcccggta ggtaaccttc
     2701 cggtcgccct gttccagggc tgtgcggtcg gggaacctgc gggcggtatc ccatagccgg
     2761 ccacccagtg aggccaggat atcttcttgc agttcggtca tggtcttaat tctgattcca
     2821 gcaataaagc gtcggcaaag aacttcgtcg cccagttcgg aaaggcaaat ggctcatagc
     2881 gcccccacag cgggaaagaa cccgggatgg cgcccagtgt atccaccggt tcaccgcgca
     2941 tcctgctctg taccgtcatc agcagatcca gcatttgacc ggccgcatcg taccagaccc
     3001 gttcgcccga aattctgaag aatttcagcc agacgatgga tatctgggcc gatccggtgc
     3061 agatcatatg ctccgaacca tgccagttcc ggtcgtatct gccatgcaaa tatccccgtt
     3121 cagggaaaat ctcccggagt tgtgcggcgc ctttccgtgc agcggctata tacttttcat
     3181 cttccagcat caatccgcaa tcgaccaggc catcgagcgt ataggcaatc gtgtgcagaa
     3241 tgggtttatc atttctccgg atggtattgt cacaatcttc aaaccagcca ttctccagca
     3301 ttttctcacc gacgacccag tcgagattac gtatagccgc atttttcagg ctttcctcac
     3361 cggtaaggcc gtacaattcg agcagcgggg catcgacgaa agtgtcgtat accctcggct
     3421 ggttcatcag ggcaaattcc ttccagtatc cctccggatt ttggattccg gataaccagc
     3481 ggccggctgc cgacgctgca tcaaggtatt tttgttcccc gctgaaattg tatgctgcca
     3541 tcatgccgcg gatcgcctgg cccgtattga aaacaatctc gggtttattt tcggccactc
     3601 ttcccccctg ccatcccccg ctcttcttct ggattttcag taaaaatttc gctgctttca
     3661 tcgcactacc ggtaacctca tcattatcat attttcttcc gaaattcaac aatgtaggaa
     3721 taatgtaccc ggtcgtttcc ggataagagg atgaccactt atttataaga tgaaaagatc
     3781 ccatcccgcc atcggtattg gcttgttgcg atctgaccag ccaggcgata gctttcataa
     3841 gcgctttagc ttgaaggtca ggttctttat gaggttcttt cttaaaaata tctttattaa
     3901 aaatatagcg cccggtatcc agccaaagat atttcccggc cggtgaaatg gccgacacta
     3961 tgaaatattt tgcatataac ctgaggctgt taagcatatt tttcagaaaa ttctaccggc
     4021 aaagataacg aagtaaggct tgtttatatt ttcccttaac taatatccgt aatccgatca
     4081 cggctggtca atctttatta tttttgacca atcgaaaagg aggaacacgc aggacatgga
     4141 taattttgtc gtttcagcaa gaaagtacag gccggccaca ttcgatatgg ttgtaggcca
     4201 gcaatccatt acatcgacct taaagaacgc gatccggaat aatcagcttg cgcaggcatt
     4261 cctgttctgc gggccaagag gagtaggcaa aacgacctgt gcacgtatcc tggccaagac
     4321 gatcaattgc atgaatccca gggacaagat agagccctgc gaccagtgtg aatcgtgtct
     4381 ttctttcaac cgttcggctt ctttcaacat ccacgagctg gatgcggcat ccaacaattc
     4441 ggtcgacgac atccgaaacc tggtcgacca ggtcaggatt ccgccgcagg ttggaaaata
     4501 caaagtgtat atcatcgatg aagtccacat gctttcggcg gcggcattca acgcttttct
     4561 gaagacgttg gaggaacctc cgccatacgc taaattcatc ctggcgacga cggaaaagca
     4621 caagatcatt ccgacgatcc tttcccgttg ccagatattt gatttcagac ggattgtagt
     4681 taaagatatt gccgattacc tggcgtatgt agctaaaagc gaaggagtcg acgcagaaga
     4741 ggaggcgttg catgtcatcg cccagaaagc cgatggtgcc atgcgcgacg ccctttcatt
     4801 cttcgaccag cttgtcagct tttcaggcag tcagcttacg tatcagactg ttaccgaaca
     4861 tcttaacatc ctggatcatg cctattattt caggatcatg gaccaggcgc tcagccagga
     4921 taccagcggc attttactga ccctggatga gatcatcagc cggggatttg atggccagca
     4981 tttcctgatc ggcctggggg aacatctccg gaacctgctg gtatgccagg acccaaaaac
     5041 ggtgaccctg atggatataa gctccaatct gcagcagaag tatgcggaac aagccggaaa
     5101 atgttcaccg gcttttttgc tccaggcgct ggagattcat aaccagtacg atcaaagcta
     5161 caaaacagcc aataacaaac ggctgcagct tgaactggtc atgctgctga tcagtgctgc
     5221 tgcagtaaaa tccgggtctc cggaaccgga aaaaaagatt tcattgattc cgcctccggc
     5281 agataaaaaa ccgggcccgg aacccgggat tcctttaacg ccaaagcccg aagaaaataa
     5341 gactgttgcg ccccctgttg ctgcggttcc caagccatcg gcagcactgt cggctagccg
     5401 caccatctcc atcaaacccg aaaatcatac gccgaaaaag gttggagcaa ccggtgaaga
     5461 aacgcctgca ggaccggtta atcagaaaga attcacacaa gagaagctgg aaaaagtctg
     5521 gctctattac acggaaagca ttgcgggtca gtatcccaat ttttacagca ttctatcgac
     5581 ccggaaacct gttctgaagg aaaatttcct gatctatctc cagttggaca ataagactca
     5641 ggagatcacc ttaaaagacc gccgcgccga tctcctggac tttttacgca acgaattagg
     5701 aaatcagtcc gttcagatgg aatccgttgt cattgagaca gcaacgcaat caaaacccta
     5761 tacgccggat gagaaattcc ggtcgatggt ggaaaagaat cccgaattaa aggttttaaa
     5821 ggaaggtctt gacctggaga tcgagttctg aagaaggagt cttaaaaaga catgttcttc
     5881 agaacccgtc caggtatgat cattattccg gatgaaccgg gtttgagata ttatcctcca
     5941 tatttgcagg catcaacacg gcttcgataa tattatcgcc aaagaattgc tgggcagccg
     6001 tttttaatga tccgaccgac atgctgttca cataatcttc atacttcata tattcatcca
     6061 ggccggtgcc atagtaatcc ttatttttaa tgattccgag ccaatagttg ttttgcctca
     6121 gattttcctg gtggactttg atcttgttct ctttcacgcc gtgcaggtct ttctccgtcg
     6181 ggccatccgt tttcagcttg ccaatttcat cataaacgat ctgtttaagt ttgccggcat
     6241 tttgcggatc acagtcaaag aatatagtaa ctgaataatt ttcgtaagga tatttgtcca
     6301 tgctctcata aaccgcggcc ccataagttc cgctctgctc ttcgcggatg gtttccacaa
     6361 atcgcacatc gagaatatcg caaagcgccg acaggttcag cctggattga aaatcatcat
     6421 aatcaaaagt accggtatat gttaaataga cggtaccttt cgggactttc atttcacgtt
     6481 cgatcacacg ttccactttg cctgcaggcg gacggatatt attgtctttg aatgtttctt
     6541 cgcgtgaaac tttcggcagt ccaccgagat atttttcgat caggggttta gcagcgacgg
     6601 gatcgatatt tccgacgaaa tagaaggtaa aactgcccgg atcgccaaag cgggttttga
     6661 agatggagcg ggccttctta agattggtct cgtcgagtat ggcagaggtc aatggccgca
     6721 cgcggggatg atggttggcc atcaggaccg tcacggtatc ctgaagggct gattcgggtt
     6781 cgagcgcttt attatccagc atagccttca ttctcttaat aaaagcatta aaggccacct
     6841 catctgttct gggagccgta aaatagagat tgaccatttg gagcatcgtt tccaggtctt
     6901 tcacagagca agaaccttca agaccctctg agctcccctt gatcaccggc cttacgctcg
     6961 cgatcacccc cgtcagtttt ttatccaggg caatccggtc gaactcgccc aatccgcttt
     7021 ccaatgaaat atcggaagca aacatagccg acatcaggtc cttggtttca tacagtgagg
     7081 atccgccaaa actataagcg ctcatcagga tctcatcatc tttaaaatcg gttgttttca
     7141 tcacggcttt taccccgttc tcaaaggtcc aggtggttgt tcccaatgtt ttatcctttc
     7201 ctttcttagc cacctttgca ggtgtaggtt ctgcagccat cagcggctta tcggtcactt
     7261 tatccacgta agccgttatg ggttccgttt caacagcggt gatgatatcc ctgacttcct
     7321 gttccgaagg cactttaata tcgggattgt cgggggccat cagtgaaacg accctgtttt
     7381 tatcaattat ccacttaccc gcctgggcat tgacctcctc cagggtaatt cccggaagca
     7441 tagcattggt aaaggcaaaa tcataagcaa cgcccggggt tggctcctgt tccaggaagt
     7501 gtgagaaata ctgccagaca tactggtcgc ttgccatttt atctttttcg gcgaattgtt
     7561 tttcaatacc tcttttcagt tcacttttgg cccgatcgaa ctctgtagta gtaaaaccaa
     7621 attctttaac tctcttgttt tcaaccagca gagcacgaag agctttgtca agttcattat
     7681 tattgccaac ggccagtgaa agaaaaccgt ccttagtcct ggcaatgttt gtataaaatg
     7741 catacccata aacaaacgga ggattttcag aaaggatcag ttcctgcagg cgggcattca
     7801 gcatggaatt aaaaagttgt tgtttgagct gttcgcggta atactccatg tttttctcat
     7861 ttgcaggctc gtgtttgtaa tagatctgaa cgatgggata ctgcgcttcc ttgtctgtga
     7921 ggatcgtcac gagggtttca tcgtggttcg gaacggggaa gtattcgcgt tcacgagggt
     7981 tatttttaac ggggatggtg ccgaagagct gtttgatatg ctgttccatc gcttttccat
     8041 caaaatcccc aacggcaata atagcttgca ggtcgggcct gtaccagtcg ctataaaaac
     8101 ggcggatcac atcatgagaa aagttatcga ttatgtcgat atcgccgatc acatcgtgca
     8161 cagcatactt ggagtcttta tagatcacct tgttggcttc gcgcatcatg cggaacatgg
     8221 cagaacggcc tgttcgccat tcttcgtgga tgactccgcg ctctgcatca atttcttcat
     8281 cttcgaaaga gatgtttgtt gcccagtcgt ataggataag gagggctgta tcgacgatct
     8341 tcggatcggt ggtcgggacc ttctgcagca tataattggt ttcgtcgctg ctggtaaaag
     8401 cgttgatttc aggcccgaac ttcatcccga tcgattgcag gtaattcagg atggctttct
     8461 tgctgaagtt ttttgtgccg ttaaaggcca tatgctctgt aaagtgggcc aggccgttct
     8521 ggtcggcatc ttccgagagt gccccgacat tcacggccag gtagaactcg gccctttctt
     8581 ttggctcgcg gttttcgcgg atgtaatagg tcattccgtt gtcgagtttt ccatagatca
     8641 catcgggatc caaagggagt accgaacccg gtgcataaga gatctcctgg gccggcaggc
     8701 tgaaggcaaa tagcagcaaa agactgctga tcagataaaa tactattctt ttcatagaaa
     8761 aaatgtttgg ttacaaaaga tgggcaaaag tagcaataaa aaaggctgat caaatttttg
     8821 atacagcctg ttttgtggga gttactggat tcgaaccagt gaccctctgc ttgtaaggct
     8881 taaat
//
LOCUS       DAOPNH010000136         8815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8767_length_8815_cov_7.686986, whole genome shotgun sequence.
ACCESSION   DAOPNH010000136 DAOPNH010000000
VERSION     DAOPNH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_8815_cov_7.686986"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="PKZ74_08355"
     CDS             complement(<1..1666)
                     /locus_tag="PKZ74_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92809.1"
                     /translation="MKAVFSLVCLTMFYSSSVVAVTRYWINGGSGNFNDGTHWSLSSG
                     GNPINGTISWAITDVAVFDYNSGSPTVIFTASEQIGKFRFYGSDTVTLKANSTYSRTL
                     GINAVDTASLLVSDVSTLIISGTASTTDRNMTVELDNATGVTAKIYGVVEVKEENSGY
                     GEFRKKGSYSTIFFRYGSTYIHNTKETGSDIPIATWHAASTCKITGITGTVPGNLDQP
                     FGNLIWNCPGQTDVFNWPTMPALLGNFTIASTGSGGIKLSDAIDITWNISGSYSQTGG
                     NFYPAGTSANNIINLGGGFSMTGGILSCPGTGSCLFRFNTSGVHNYSKTGGTYYQKIH
                     FEVVSGATLDMGGSIIDNGSSGNFTLNSGGCLKTAHVQGISSTGASGCIQLTGSRTFN
                     AGASYIFYKSGSQATGTGLPATLSSVLTIGTIHNATSLTLTNSPVTINGTLVLVSDSA
                     INSAIASGTISYGSNGTLEYQGDTAQVTTDIEFPSTSGPYNLKINNAYGVSLHEDRTI
                     NGTIYLSIGQFLIGAHTLTINGTLSQTMGVLKGGLSSNIIVGGTGNL"
     gene            1789..2394
                     /locus_tag="PKZ74_08360"
     CDS             1789..2394
                     /locus_tag="PKZ74_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPM92810.1"
                     /translation="MKTLLLISIFIFLAYTGKTQTVTDTDGNTYPTVKIGEQTWMAKN
                     LAVTRAPDGTVLESYPCNGIEDSVKTFGRMYDWANAKKACPAGWHLPTDEEWTQLVDF
                     LGGPMIAGGKIKETGYAHWKAPNAGADNKSGFTALPGGYRNPKGKYFNFKNNLAYFWT
                     ATEYDEANAWGYYITYGEPIIYRYGMSFGKKMAFAVRCIKD"
     gene            2559..4637
                     /gene="fusA"
                     /locus_tag="PKZ74_08365"
     CDS             2559..4637
                     /gene="fusA"
                     /locus_tag="PKZ74_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM92811.1"
                     /translation="MPLNRLRNIGIAAHIDAGKTTVTERILFFTGVTRKIGEVHDGQA
                     TTDFMKQEQERGITIASAAISCAWKDSQINLIDTPGHVDFTVEVERSLRVIDGMIALF
                     CAVGGVEPQSETVWNQADRYKVPRIAFINKMDRTGADFYDVVGQMNNVLDANAIAFQI
                     PIGVEDDFTGIIDIISGKAYKFTDKGREEISIPGEYTEKVNEARHQLIEKLADFNDEI
                     AELFLDDGEISNELLKKAAREATLRLNITPVFCGAAYKNKGVQLLLDAVIDYLPSPLD
                     IGAIVGTDVDNPEKSHARHPSANDPFSALAFKLINDPYVGQQTFVRVYSGQLKSGMQV
                     LNSTKDKTERIGRIMKIHAKDREEISDAGPGDIVALVGLKLTKTGDTLCDPAHPLHLE
                     SITVPATVIEMKILPTSRKDQSKLGEALHKLSNEDPSFNVRYDEETEETIIAGMGELH
                     LEIIIDRLKHEFGVEVDVSEPSVAYRETITTEVEASHRYSKQTGGKGQFAETHFRIEP
                     NEGNGYEFIDRIKGGAIPMEFVPSVNKGITKTLEKGVLAGFPVVDVRIVLHDGKFHPV
                     DSSDMAFQTCASICFKEGFMKAHPILLEPIMKVEINTPDDYIGDIVGNLNKRRGKVES
                     MRRHRKGSQKVVAFVPLVEMFGYATQLRNISSGRASFSMDFFRYMPLSKTVQEEVLKK
                     LAEKKAAMKK"
     gene            4692..5615
                     /locus_tag="PKZ74_08370"
     CDS             4692..5615
                     /locus_tag="PKZ74_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM92812.1"
                     /translation="MQTILGSTGIIGKEVARELYRSYTKDIRLVSRNPKPVNPTDQLF
                     KADLLDRDQVRRAVEGSEIVYLTVGIQYSAKLWAEQWPVIMRNVIEACKAEKAKLVFF
                     DNVYAYGKVDGVMTEETPYNPCSKKGTVRLKVINMMMDEVRKGNLEGLIARAPDFYGP
                     DTPLSVVSVTVFGNLAKGKEPQWMVDDRKKHSFIFTPDAGKATALLGNTPSAFNQVWH
                     LPTDSNTLTGKEFVVLAARAFGMKPSYMVLPKWLLRTVGLFNPIIRESLEMLYQNEVD
                     YIFDSSKFEKAFGFAPTPYPTGIEETARSYK"
     gene            5742..6665
                     /locus_tag="PKZ74_08375"
     CDS             5742..6665
                     /locus_tag="PKZ74_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM92813.1"
                     /translation="MPIPTSRTYESGHVVIDQEKCTGCGLCAEVCSDAVIEIIDGKAV
                     ATQKNSVFGCIGCGQCMAVCPEGAVSVTGRWISPDDVFPLADRSESASYDSLLKLYQR
                     RRSIRDFKDKPVEPELVEKILQAAVTAPMGIPPSDVNVLVLNSKEKVFQFSKDYCDYL
                     ENLKWMVSPLGLLFMRLFYGKENGDMFRDFIKPVINAYTGNMKEGKNIVMYDAPLAFY
                     FYGSPYTDPADPIIACTYAMHAAESLGLGTCMLGAIHPFIQSGGAAKKFREKHGIRFK
                     SKEGLFLIVGYPKVKFRNGIKRTFAGVEGLN"
     gene            complement(6669..7715)
                     /locus_tag="PKZ74_08380"
     CDS             complement(6669..7715)
                     /locus_tag="PKZ74_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HPM92814.1"
                     /translation="MIEFTNCNIDRISIHGIGNKTNGEDLTISNSIVEIGDIRARELL
                     YKFFLSSFSDPEYYSFTFSNEDFTLNPVYIFSKVIFDNVNLFHDTTKDLAKLLYELSV
                     HPQIKAGDLFVAYFTGISLDNIIVDGIGLFKSENKQSFLKLQSEGYSYSINHDEGINI
                     EKLDKGCLILNTQNDKGYKICLVDKSNKSAETQYWKDDFLKVKPIANEFFQTNQFLSV
                     AKNFVTKQFSKEFEVSRAEQIDLLNKSVEYFKLHETFDKNEFETDVFKNQEVIDSFRN
                     FDSQFRQDNDIELSDQFDISQKAVKKQARIFKSVLKLDKNFHIYIHGDRELIQQGVDP
                     DGRKYYKIYFKEES"
     gene            complement(7721..8698)
                     /locus_tag="PKZ74_08385"
     CDS             complement(7721..8698)
                     /locus_tag="PKZ74_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92815.1"
                     /translation="MMDIKVIAEAWKGDQPKYEELGQLVNKFIRSTITECEILPEITF
                     RTKELYSIVKKIKKKLREKEYSYTDLNDKLGIRIICTFQEEMEVIEKFLYKYFEIKKV
                     ERKRETLDIHTLDYTSNHYDACIKTSIKQFTKYSTLKNLVFEIQVRTLNQHAWANTAH
                     ALSYKQEANIPPNLERKVYRLLSLYEIADDEFSVVNSSLKQLPDNEIYVLLRKLEGKI
                     YKYAQVDFDRELSLNVFRTILGFLEPQNAKLLSLEIENFIKINSDKIQQIFAENEGRF
                     YEIPLLTQPEIFIIWYCLNKFPYSFKDKWEDNFDADELEQIEILWGKQI"
BASE COUNT         2446 a         2121 c         1886 g         2362 t
ORIGIN      
        1 gaaggtttcc tgttccaccg acgatgatat tggagctcaa tccccccttc aatacaccca
       61 ttgtttgtga cagggttcca ttaattgtca gtgtatgagc cccgataaga aattgcccta
      121 ttgaaaggta aatcgtgccg tttatggtcc ggtcttcatg aagggaaacg ccatatgcat
      181 tgttaatttt taaattataa ggacctgaag tcgaaggaaa ctctatgtct gtcgttactt
      241 gcgccgtgtc accctggtat tcgagtgtcc cattgcttcc ataacttatg gtgccggagg
      301 cgatcgcact gttgattgca gaatcactta caagaacaag cgttccgtta atggttacag
      361 gagaattggt aagtgtaagg cttgtggcat tatggattgt tccaatcgtg aggactgaac
      421 taagcgttgc cggcagacca gtccctgtag cctggctgcc actcttataa aaaatataac
      481 tggcgccggc attaaaggta cgggaacctg tgagctgtat acaaccggat gcgccggtgc
      541 tggatattcc ctggacatgt gcggttttca ggcatccccc tgaatttaat gtgaaattgc
      601 ccgaagaacc attatcaata atcgatcctc ccatatccaa tgtagctccg cttacgactt
      661 caaaatggat cttctgataa taggttcctc cggttttgct atagttatgt actccggaag
      721 tattaaaccg gaagaggcaa ctgcctgttc ccgggcagga taggatccca cctgtcatgc
      781 taaatcctcc ccccaggttt atgatgttgt tcgcactggt tccggctgga taaaaattac
      841 cccctgtctg tgaataactt ccgcttatat tccaggtaat atctatagcg tccgataatt
      901 ttatccctcc acttcccgtg ctcgcaatgg taaaattacc cagcaatgca ggcatggtcg
      961 gccagttaaa aacatctgtc tgcccggggc agttccaaat caggtttcca aagggttggt
     1021 ccaggtttcc gggcacggtg cccgtaatcc ccgttatttt gcaggttgag gcggcatgcc
     1081 aggttgcaat ggggatatcg ctgcccgtct cttttgtatt atgtatgtag gtactcccgt
     1141 atctgaaaaa gatcgtggag tatgatcctt tcttacggaa ctctccataa cccgaattct
     1201 cctccttaac ttcgacgacg ccgtaaatct tcgctgtaac ccccgtcgca ttgtctaact
     1261 ccacggtcat atttcgatca gtagtggaag ccgtgccgga aatgatcagt gtcgatacat
     1321 cgctgacaag taatgaagcg gtgtcgacag cgttgatgcc taacgtacgg ctgtatgtac
     1381 tgttggcttt cagggtgacc gtatcggacc cgtaaaatcg aaatttcccg atctgttcgc
     1441 tggcagtgaa gataactgtt ggagaacccg aattataatc aaatactgca acatctgtta
     1501 tggcccagct tatcgtaccg tttattggat ttcctcctga agaaagcgac cagtgtgtcc
     1561 cgtcgttgaa attgccggaa ccgccgttga tccagtacct ggtaactgca acaacagagg
     1621 aagaataaaa catagtaaga caaaccaagg agaaaactgc tttcataatt cctccttttt
     1681 aattaaatcc tttaaaaaca ttaaagttat gaaatttgaa aacttaaatt aagaaaacct
     1741 aatttcggtt taaagtgttt ttataacttc gtttaaaaat acggtgtgat gaaaaccctc
     1801 ctgctgattt ccatttttat ttttctggca tatacaggaa aaacccagac cgtcaccgat
     1861 accgacggta acacttaccc cactgtcaaa attggagaac agacctggat ggcaaagaat
     1921 ctggcggtca ccagagcgcc cgacggcacg gtcctcgaat cgtatccctg caatggaatt
     1981 gaagacagcg taaaaacttt cggccggatg tacgactggg ccaatgccaa aaaagcctgc
     2041 ccggcaggat ggcacctgcc caccgatgaa gaatggaccc aattggtaga tttcctgggc
     2101 gggcccatga tcgccggagg caagatcaaa gagaccggat atgctcactg gaaagccccc
     2161 aatgccggcg ccgacaacaa atcgggattc accgccctgc ccggtggtta caggaaccca
     2221 aaaggaaaat acttcaattt caagaataac ctggcctatt tctggaccgc gacagaatac
     2281 gatgaagcca atgcctgggg ttattacatc acctacggtg agccgatcat ctaccgctat
     2341 ggcatgtcat tcggcaaaaa gatggccttc gccgtcaggt gcataaaaga ttaaaaatta
     2401 aatatttgtt atctttcata tgaagtttgc gttttgcaac ttgattatca ctacctttgc
     2461 aaactttttc ggcccccacc ccttgatccc ctcccccaat tgggggaggg gcccggggag
     2521 ggggcacatt caaaattcaa catttaaaaa ttaccattgt gcctttaaac agattacgca
     2581 acatcggcat tgccgcccat atcgatgccg gtaaaactac cgttaccgaa cgaatattat
     2641 tctttacagg tgtcacacgc aaaattggcg aggttcacga cgggcaggcg acgaccgact
     2701 tcatgaagca ggagcaggag cgtggcatta ccattgcctc tgccgccatt tcatgtgcct
     2761 ggaaagattc ccagataaac ctcatcgata ctcccggaca cgtcgatttt accgtggagg
     2821 tggaacgctc cctccgcgtg atcgacggga tgatcgccct gttctgcgcc gtgggaggcg
     2881 tggaaccgca gagcgaaaca gtatggaacc aggccgaccg gtacaaggtt ccccggatcg
     2941 catttatcaa taaaatggac cgcaccggtg ccgacttcta tgacgtcgtc ggccagatga
     3001 ataatgtcct cgacgctaac gccatcgctt tccagatccc gatcggggtc gaagacgatt
     3061 ttaccggcat catcgacatc atatccggta aagcttataa attcaccgac aaaggacgcg
     3121 aagaaatatc catccccggc gaatacaccg aaaaagtgaa cgaggcccgc caccagctta
     3181 tcgagaaact ggccgacttc aacgatgaga tcgccgaact tttcctggat gacggcgaaa
     3241 tcagcaacga actgcttaaa aaagccgccc gcgaagcaac cctccgcctg aacatcacac
     3301 cggtgttctg cggtgcagcc tacaaaaaca agggcgtcca gctcctgctc gacgctgtga
     3361 tcgactacct cccctccccg ctcgatattg gagccattgt gggaaccgac gtcgacaacc
     3421 ctgaaaaatc gcacgcccgg cacccttcag ccaacgatcc tttctccgcc ctggcattta
     3481 aactgatcaa cgacccctat gtcggacagc aaacgttcgt ccgtgtctat tccggtcaac
     3541 tgaaaagcgg catgcaggtg ctgaattcca ccaaagacaa aacagaacgg atcggccgca
     3601 tcatgaagat ccacgccaaa gaccgcgaag agatcagcga tgccggcccc ggagacatcg
     3661 tagccctggt gggactgaag ctgaccaaaa ccggcgatac gctctgcgat ccggcccatc
     3721 cgctccacct ggaatcgatc acagtcccgg caacggtcat cgaaatgaag atccttccaa
     3781 cctcacggaa agaccagagc aaactgggcg aagcgctcca caagctttcc aatgaggatc
     3841 cgtcgttcaa cgtaaggtac gacgaggaaa cggaagagac gatcattgcc ggcatgggtg
     3901 aacttcacct ggagatcatc atcgaccgcc tcaaacacga attcggcgtg gaggtcgatg
     3961 tcagtgaacc ctctgtcgct tatcgcgaaa ctattaccac agaagtcgag gcttcgcacc
     4021 gctacagcaa acagaccggg ggcaaaggcc agttcgccga gacccacttc cgcatcgagc
     4081 ctaacgaagg caatgggtac gagtttatcg acaggatcaa gggaggagct atccctatgg
     4141 aatttgtccc atccgttaac aaaggaatta ctaagactct cgaaaaaggg gtcctggccg
     4201 gattcccggt ggtcgacgtc cggatcgtgc tccacgacgg gaaattccat ccggttgatt
     4261 cgagcgatat ggccttccag acctgtgcct cgatctgctt caaggaagga tttatgaaag
     4321 cacacccgat cctcctcgaa ccgatcatga aggtcgagat caatacaccg gatgattaca
     4381 tcggcgatat tgtcggcaac ctcaacaagc gccgcggcaa agtcgaatcg atgcgccgtc
     4441 accgcaaagg ctcgcagaag gtcgtcgcct tcgtcccgct ggtagaaatg ttcggctatg
     4501 ctacacaatt gcgtaatatt tccagcggcc gcgccagctt ctccatggat ttcttccgct
     4561 acatgccact cagcaaaacg gtgcaggaag aggtgctgaa gaaactggcc gagaaaaaag
     4621 cagcgatgaa gaaatagttt ttcgacttgt cagcgtccgc aggacctgac aatgccaaaa
     4681 caaaaagaaa tatgcaaaca atcctaggct caaccggaat catcggcaaa gaagttgccc
     4741 gcgaacttta tcgttcctac accaaagaca ttcgtctggt atcccgtaac ccgaaacccg
     4801 tcaacccgac cgaccagctt ttcaaagcgg atctgctcga cagagaccag gtacggcggg
     4861 ctgttgaagg ttccgaaatc gtttacctga cggtcggtat ccagtacagc gcaaaattat
     4921 gggccgagca atggcccgtg atcatgcgga acgtcatcga agcctgcaag gctgagaaag
     4981 caaaactcgt tttcttcgac aatgtttatg cctatggcaa agtagatggc gtgatgaccg
     5041 aggaaacgcc gtacaatccc tgcagcaaaa aaggaacagt gaggctgaag gtgatcaata
     5101 tgatgatgga tgaggtgagg aaaggcaacc tggaggggct catcgcccgc gccccggact
     5161 tctatggccc cgacacgccg ttgagcgttg tgagcgtaac ggttttcggc aacctggcca
     5221 aaggcaagga acctcagtgg atggttgatg accgtaaaaa gcattcgttc atcttcacgc
     5281 ccgatgcggg taaagccact gccctgctgg gcaacacccc ttccgctttc aaccaggtct
     5341 ggcatcttcc gaccgattcc aacaccctca ccgggaaaga attcgtcgta ctggcagcca
     5401 gggccttcgg catgaagcca tcgtacatgg tcctcccaaa atggttgctc agaaccgtag
     5461 gattattcaa ccccatcatc cgggaaagcc tcgaaatgct ctaccagaac gaagtggatt
     5521 acattttcga cagcagcaaa ttcgaaaaag cgtttgggtt tgcgccgact ccatatccga
     5581 ctggaattga ggagactgcc agatcgtaca aataagatcc gtgttaatcc gcataatccg
     5641 cgtcatccgt gtgctattat tgctgatagc acgcggataa acacggatgc ggcggatcaa
     5701 cgcggattat tattaatttt aaggcataaa tcaaaccgat tatgcccata ccaacctcca
     5761 gaacctatga gtcgggtcat gttgttatcg accaggaaaa gtgcaccggc tgcggattat
     5821 gtgccgaagt ttgctccgat gcggttatag agataattga cggcaaagcc gtcgcaaccc
     5881 agaaaaattc cgtcttcggc tgtatcggat gcggccagtg catggctgtc tgccccgaag
     5941 gagcagtcag cgtcaccggg agatggattt cccctgacga tgtattcccg ctggccgacc
     6001 gatcggaatc ggcttcgtat gattcgttgc taaagctata ccagcgccgc cgcagtatcc
     6061 gcgatttcaa agacaaaccc gtggagccgg agctggtcga aaaaatcctt caggccgcgg
     6121 ttaccgctcc gatgggcatt ccgccgtcgg atgttaacgt gctggtattg aatagtaaag
     6181 agaaggtttt tcagttctca aaagattatt gtgattacct tgaaaacctg aaatggatgg
     6241 tgtcgcccct aggcctgctt tttatgcggc tgttctacgg aaaagagaac ggcgatatgt
     6301 tccgcgactt catcaaaccg gtcatcaacg cctataccgg aaacatgaaa gaagggaaga
     6361 acattgtgat gtacgatgcc ccgctggctt tctattttta cggttcgccc tacaccgatc
     6421 ccgccgatcc gatcattgcc tgcacttacg ccatgcatgc cgccgaatcg ctcgggctgg
     6481 gcacctgcat gctcggcgcc atccacccgt tcatccagtc gggcggcgcc gcaaagaagt
     6541 tccgcgaaaa gcacggcatc cggtttaaaa gcaaggaggg cctgtttctg atcgttggtt
     6601 atccaaaagt taaattccgg aacgggatta aaaggacgtt tgcgggggtg gaaggcttaa
     6661 actgaaaatc agctttcttc tttaaagtag attttataat acttccggcc atcaggatca
     6721 acaccttgct ggattaactc tctatctccg tggatatata tgtgaaaatt cttatccagc
     6781 ttaaggacac ttttaaaaat ccttgcttgc tttttcaccg ccttttgcga tatatcaaac
     6841 tgatcagaaa gctcaatgtc attatcttgt ctaaactgag aatcaaaatt tctaaacgaa
     6901 tcaattactt cctgattttt aaaaacatcc gtttcaaatt catttttatc aaaagtctca
     6961 tgtaatttga aatactccac agatttattc agtaagtcta tctgctcagc tctgctaact
     7021 tcaaattcct ttgaaaattg tttagttaca aagtttttag ctacacttaa gaattgattt
     7081 gtttgaaaaa attcatttgc aatcggtttg acctttaaaa agtcatcctt ccaatattgg
     7141 gtttcggctg atttatttga tttatctacc aaacaaattt tgtatccttt atcgttctga
     7201 gtatttaaaa ttaaacaccc tttatctaac ttttctatgt taatcccttc atcatgattt
     7261 atagaatagc tatatccttc actttgaagt tttaaaaaag attgcttatt ttcagattta
     7321 aataagccaa taccatctac tataatatta tctagactaa ttccagtaaa atacgccaca
     7381 aataaatccc cggcttttat ttgaggatgt actgacaatt cataaaggag cttggcaaga
     7441 tcctttgtgg tgtcatgaaa caaattcaca ttatcaaaaa ttactttaga aaatatataa
     7501 acaggattta atgtaaaatc ttcatttgaa aaagtaaatg aataatattc aggatctgag
     7561 aacgaagaga ggaagaattt atacagtaac tctcttgctc ttatatcacc tatttcaact
     7621 attgaattgg aaattgttag gtcttcccca ttcgttttat taccaattcc atgtatagaa
     7681 attctatcaa tattacaatt tgtaaactca atcatagata tcaaatttgt ttgccccaaa
     7741 gtatttcaat ttgctccagt tcgtcagcat caaaattatc ctcccattta tctttaaaag
     7801 aatatgggaa tttatttaaa caataccaaa taataaatat ttcaggttgg gttagtagcg
     7861 ggatttcata aaagcgccct tcattttcgg caaatatttg ttgaatttta tcactattga
     7921 ttttgataaa attttcaatt tccagtgata aaagttttgc attttgtggt tccaaaaatc
     7981 ctagaatggt tcggaataca tttaaagaca gttctctgtc aaaatcaacc tgagcatatt
     8041 tatatatctt cccttccagc ttcctaagta atacatatat ttcattatct ggtaattgct
     8101 tcaaactact atttacaact gaaaactcat catcggcgat ttcatataaa gaaagtaacc
     8161 tgtaaacttt tctctccaag tttggtggaa tattggcctc ttgtttatat gacaatgcat
     8221 gtgctgtatt tgcccatgcg tgttgattta aggttcgaac ttgaatttca aatactagat
     8281 tttttaatgt gctatactta gtaaattgtt taatactagt cttgatgcag gcatcataat
     8341 gatttgaagt ataatctaac gtatgaatat caagagtttc cctttttctt tcaactttct
     8401 ttatttcaaa atacttataa aggaattttt caattacctc catttcctct tgaaatgtgc
     8461 aaatgatcct aattcctaat ttatcattaa gatcggtata ggaatattct ttctctctca
     8521 gcttcttctt tatcttcttt acaatactat agagctcctt ggttctaaat gtgatttctg
     8581 gtaatatctc acattcagta attgtgcttc taatgaattt atttactaat tgccccaatt
     8641 cttcatattt aggttggtcg cctttccaag cttcggcaat tacttttata tccatcattt
     8701 tatataatgt ttttaatttt agaaattact tcagcctttg tattcccatt ttctaatttt
     8761 tctagtgtta tgttaaacat gaattgacgg atataatcgt ttaagtttta ttctt
//
LOCUS       DAOPNH010000137         8805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8783_length_8805_cov_7.487429, whole genome shotgun sequence.
ACCESSION   DAOPNH010000137 DAOPNH010000000
VERSION     DAOPNH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_8805_cov_7.487429"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKZ74_08390"
     CDS             complement(<1..959)
                     /locus_tag="PKZ74_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92816.1"
                     /translation="MTAEEKILQAQEYTADNIQVLEGLEAVRKRPAMYIGDISQRGLH
                     HLIHEVVDNSIDEALGGYCDRIDLTIHEGNSITVLDNGRGIPTGLHEKENRSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSKKLVATVYREGKIFRQEYNFGKPVYD
                     VQVIGDSDKTGTQVTFTPDDSIFTVTEFDYDLISARLRELAFLNKGIRITLTDERQPN
                     EDGSFHSTSFYSEEGLKDFIKYLDVNREALMPDPITIEGERGAIPMEIALQYNTSFAE
                     NIHAYVNNINTHEGGTHLAGFRRGLTRTLKNYAEKAGLLAKLK"
     gene            complement(1216..2913)
                     /locus_tag="PKZ74_08395"
     CDS             complement(1216..2913)
                     /locus_tag="PKZ74_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92817.1"
                     /translation="MTRIIILRAILLPVLAVIMLAAGMPLASGQGKNEEVTIIAPYIP
                     SIGDATKISFKPEIKPEDNDTAKFSFSYTDRPYPVHIDPQPLEAIKYGQGDKPQLYRN
                     FAKIGIGNYYTPYLDFMATSLQSEKLLFGARLKHHSSQGKIKGYPVSAYSHNLIDIFG
                     KTFFKSHTLSLEAGYDRDVVHFYGFQPDSFPAFEFTKENIRQRFQHLDAGIEFSSNYR
                     ENDKLNHSIRLGVDHFTDLYGSRETEVGFKTRFDKSFKAGRSDFNHAVALDFGLDFLN
                     FRDSISSSSPFFMQFKPVYRLHFGQYRFMAGIDVSLAATDSPGGSSVGIDVFPLLKAE
                     VIVIPSQLSAYAEISGERTINSFRNLAGINPFIISNPQVSFTDEQFSIGGGITGNAAG
                     INFHVSAAYSYINDMALFVTDTALVPENRFKVIYDDLNRVKIQAGLGYVKTGRFSARM
                     NAAYYYYKPENELKAWHLPDYEVDLDASYTFLERYTAKLSLLALGSKYAPVYASGETA
                     SEKLNGALDLGAGAEYEISHMLTAWLDINNILNQHYQRWQEYPVQGILIMAGVKLSF"
     gene            complement(2941..5958)
                     /locus_tag="PKZ74_08400"
     CDS             complement(2941..5958)
                     /locus_tag="PKZ74_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92818.1"
                     /translation="MHSNLLRNFCLTVVAITAMTPAFPQENAASRQPASEYRLADDLF
                     RKGNLGAARRIYEQIAGNYAGQDPDLMAFAEYRQAVTAAELTHNDAAARIDRFIDKNP
                     ENALAGEAGLYLGRSFFYDNKFKDAIKAFQKADLTGLSKTARDEMNFMIGYANLKLNE
                     PASAKTYFQRVKNPKSAYFNQALYYIAHIDYIQGNYQEALIGFSGIEDDPRYAKIAPL
                     YKLQVYHYLGDYDQVIATGPALITSEGNINKPEIARLTGNAYFNRNDFEKAAEYFEIY
                     EEANRRSLSREDHYLAGFVNYKSGDYKTAVNHFQQAVRESDELSQNAGYYLGICYNES
                     GQKKYAAGAFLASYKIGINKELAEEALFNYIKIGFESPFNPYNESIALLENYLRDNPG
                     STRADEAYDYLTRLYLTSRNYKQALASIESIGEKNEKLQAAYQKILFYRAIELTNAGD
                     PDGALDLFQKAAAIKKDETIKTESLFWIGEIFYKKGNYPSAKKYYFDFLGAKKAKQSG
                     LYSRAVYNLGYVYFNSQEYDDAITQFKKYLDTPDEEDGKLTADAYLRLGDAYFISKKY
                     PEAITYYDKVILAKDAALDYALYHKALAEGAKGDFNRKIDVLKILINNYPKSSYHDDA
                     IFETAMSYILINQESQALTFFDKLIQAYPTSAKAIQASLRKGFIYFNNNDYSQAIRSF
                     KNVVEKYPGTQESQEALAALKNIYVETGDVDQYYAYARSLTFAEVSSSEEDSLSYAVA
                     ENFYMQGRCDQAIASFKKYLDSFAAGMYTTHAKFYQAQCFLKSNQLDPALARFKEVGQ
                     GPRSQFTEPALATASSMEFTLGNYGAALPLFEQLETDAGDPDNVAAAITGQMRCHFKL
                     GNYPAAALAAQKIMATGTATPELTGEARYILTKGYLAQGNIQAAESELVILSKMTGTE
                     YGAEASYHLAEIAFNSDRLQDAETLVYALSENYTAYDYWVARGFILLADVFTKSGNVF
                     QARQTLQSIIDNYQGPELGEIAREKLKSLEIKE"
     gene            complement(6114..6543)
                     /gene="rnpB"
                     /locus_tag="PKZ74_08405"
     ncRNA           complement(6114..6543)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKZ74_08405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6746..8080
                     /locus_tag="PKZ74_08410"
     CDS             6746..8080
                     /locus_tag="PKZ74_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM92819.1"
                     /translation="MRDLSVGNERGIIFRFALPMLIGNVFQQLYTLINSIIVGQFVGK
                     EALAAVGASFPMIFTLISLVIGIAIGATIIISQYFGAKDMRNVLRAIDTLLVFTLLAS
                     VVVTITGILISKPIFRLIELPEEVFPLAVSYFNIYMLGLVFAFGFNSIAAILRGMGDS
                     KTPLYFLVISTLLNIGLDLLFVLGFGWGVKGVAWATNISQAVALLFAILYLNRTHDIF
                     KVRWRKPDFDRAILNKSLRIGFPVGFQQAFVALSMLAMYWLVNRFGTDVAAAFSVAFR
                     IDSFASMPAMNFAMALSAFVGQNLGARKPERVKRGLVATFYMTAIISVTISLVGIFFA
                     GPLMRLFTTDAAVVEVGKDYLQIVTGFYIVFSSMFVVGGVMRGAGDTFIPMLITFFAL
                     WVVRIPVGYFLSEKMGYHGIWWCIPIAWVVGLGLSFAYYSTGRWRKKVVLLV"
     gene            8175..>8805
                     /locus_tag="PKZ74_08415"
     CDS             8175..>8805
                     /locus_tag="PKZ74_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92820.1"
                     /translation="MKRIFFKTRAIAGLLMVLFFIKTLIVNSQSIEIYQTFISDAEIQ
                     PFTPEDTIYGITVSGLVNLKSDTSLVRIILSDINGTKWMIHESYPLIASIKEFNMEND
                     CDETCYLDETLPVSITIQIIDAELYISTFSLSTIPSENPSLLQYQAKRSKDLEKVQQM
                     NINIQATGWNWTADTTNLVKMFYKEKIKNFDEKFNLLGLEFYSGGIYKSI"
BASE COUNT         2023 a         2029 c         2310 g         2443 t
ORIGIN      
        1 aacttcagct tggccagcag gccggccttt tcggcgtaat ttttcagggt ccgggtcagt
       61 cctctccgga aacccgccag gtgcgttccg ccttcgtggg tgttgatgtt attgacgtag
      121 gcgtggatgt tctcggcaaa tgaggtattg tattggagtg caatttccat tgggatggcg
      181 ccccgttccc cctctattgt gatgggatcg ggcatcagcg cttcacggtt cacgtcgagg
      241 tatttgataa agtccttaag cccttcttcc gaataaaaag aagtcgaatg gaaactgccg
      301 tcttcattag gttgcctctc atcggtcagc gtgatccgga tccctttgtt gagaaaggcc
      361 agttcgcgaa gcctggctga gatcaggtcg taatcgaatt cggtgacggt aaagatcgaa
      421 tcatcggggg taaaagtgac ctgtgtgcct gttttgtccg agtcgccaat cacctggaca
      481 tcgtaaaccg gtttcccgaa gttatattcc tgacggaata tttttccttc gcggtaaacc
      541 gttgctacca gcttttttga aagggcgttc acgcacgata cccctacgcc gtgaagaccg
      601 ccggacactt tgtaagatcc tttgtcgaat tttccaccgg catggaggac ggtcatgacc
      661 acttcgagtg ccgacctgtt ctctttttcg tgcaggcctg tagggatacc acgtccattg
      721 tcaagaacgg taatcgagtt gccttcgtgt atggtcaggt cgatccggtc gcaatacccg
      781 ccaagggctt cgtcgatcga gttgtctact acttcatgga tcaggtggtg caatccgcgc
      841 tggctgatat ccccgatgta catggccggg cgtttccgta cggcctccag gccctccaaa
      901 acctgtatgt tgtctgctgt atattcctga gcctgtaaaa tcttttcttc tgcagtcata
      961 atcaattagt tacaaataaa tagataaggt tacaaattta cggattttat ccggattaaa
     1021 gttgttaaaa catcagtaaa atcaacctat ttattaacaa tttggtgatc gttttttaag
     1081 aagggatgga aggatagcta gatgggttcg gttgtccgac gggtaaatcc gtcggtgaga
     1141 gatgaggtag tccgacgact aaagccgtcg tgagagatga ggtagtccga cgactaaagc
     1201 cgtcggttcc tacctttaaa aagataattt aaccccggcc ataataagga tgccctggac
     1261 gggatattcc tgccagcgct ggtaatgctg gttcaggatg ttattgatat cgagccaggc
     1321 cgtgagcata tggctgatct catattcggc gccggcgcct aaatcgagtg cgccgttgag
     1381 cttttccgaa gcggtttcgc cggatgcgta aaccggggca tattttgagc ccagggccag
     1441 caaagaaagc tttgccgtat atctttccag gaaagtgtac gatgcatcca gatccacttc
     1501 ataatcaggc aggtgccatg ctttcaattc attctcaggt ttataataat agtaagccgc
     1561 attcatccgt gccgaaaacc ggcctgtttt aacatagccc agtccagcct ggatcttcac
     1621 ccggttcagg tcgtcgtaaa tgactttaaa cctgttttcg ggcaccaggg ctgtatcggt
     1681 gacgaacagg gccatatcgt tgatgtaaga ataggcggcc gatacatgga aattgatgcc
     1741 ggcggcattg ccggtgatcc cgccgccaat ggagaactgc tcgtcagtaa atgaaacctg
     1801 cgggtttgag atgataaacg ggttgatgcc ggccaggttc cggaaagaat tgatcgttcg
     1861 ttctcccgaa atttcggcat acgcgctgag ttgcgaaggg atgacgatga cctcggcctt
     1921 caggagcggg aatacgtcga tcccaaccga tgaaccgccc ggtgaatcgg tagccgccag
     1981 gcttacatca atgccggcca taaagcggta ttggccaaaa tgcaaccggt aaaccggttt
     2041 gaattgcata aagaaagggg aagaggagga gatggagtct ctgaaattca gaaaatccag
     2101 cccgaaatcc agtgccactg catggttgaa gtcgctacgg cctgctttga aggatttatc
     2161 aaagcgggtt ttaaatccca cttcggtttc gcgcgagccg taaaggtcgg tgaaatgatc
     2221 aactcccagc cggatcgaat ggttcagttt gtcgttctcc cggtaattgc tgctgaattc
     2281 gataccggca tccagatgct ggaaccgctg ccggatattc tcctttgtga actcgaacgc
     2341 cggaaaactg tcgggctgga aaccgtagaa atggacgaca tccctgtcgt atcccgcctc
     2401 cagtgacagg gtgtgcgatt taaagaatgt ctttccaaaa atatcaatca ggttgtgact
     2461 gtaagcgctg acggggtaac ctttgatctt tccctgcgac gaatggtgtt tgagccgtgc
     2521 gccgaaaagc aatttttcgg attgcagcga ggtagccata aaatcgaggt atggggtata
     2581 gtaattacca atgccgattt ttgcaaaatt gcgatatagc tgaggtttgt cgccctggcc
     2641 gtatttgatc gcttccagcg gctgcgggtc gatatgaaca ggataaggcc ggtcggtata
     2701 ggaaaagctg aatttagccg tatcgttatc ttccggtttt atttcgggtt tgaaagaaat
     2761 cttggttgca tcgccaatcg atgggatgta aggtgcgatg atcgtcacct cttcattctt
     2821 tccctgccct gaagccagtg gcataccggc agccagcatg atgaccgcca gtaccggcag
     2881 cagaattgct cttaatatta tgatccgcgt catagtttct aattctatta tcggggaaag
     2941 ttattctttg atttcgaggg atttgagttt ttctctggcg atttcgccca gttccggacc
     3001 ctggtagtta tcgatgatgc tctgcaaggt ttgccgggcc tggaaaacat taccgctttt
     3061 ggtaaaaaca tcggccagga ggataaatcc gcgtgctacc cagtaatcgt aggcagtata
     3121 gttttccgac agggcataaa cgagtgtttc tgcatcctgt aaccggtctg aattaaaggc
     3181 gatctcggcc aggtgatagc tggcttcggc gccatattcc gtacctgtca ttttgctgag
     3241 gatcaccagc tcgctttcgg cggcctggat attaccctgt gcgagataac ctttggtgag
     3301 gatatagcgg gcttcgcccg tgagttcggg tgttgccgta cccgtagcca taattttctg
     3361 agcggccagg gctgcagcgg gataatttcc gagtttgaaa tggcaacgca tctgacctgt
     3421 gatggctgcc gctacattat cgggatctcc ggcatcggtc tccaattgct cgaagagcgg
     3481 tagggcagcc ccgtaattgc ccagcgtaaa ttccattgag gaagctgtgg ccagggccgg
     3541 ttcggtgaac tgcgaccgcg gtccctgtcc cacctcttta aaccgggcca acgccggatc
     3601 aagctggtta gacttcagga agcactgggc ctggtaaaac tttgcatgag tggtatacat
     3661 ccctgctgca aagctgtcga ggtatttttt aaaggacgcg atagcctggt cgcatcgtcc
     3721 ctgcatataa aaattctcgg ctacagcgta gctgagcgaa tcttcttcgg aagaactgac
     3781 ctcggcaaaa gtcaggctgc gggcataggc gtaatactgg tcgacgtcgc cggtctccac
     3841 atagatgttt ttcagtgccg ccagtgcttc ctgtgattcc tgcgtaccgg gatacttctc
     3901 cacgacattt ttgaatgagc ggatcgcctg gctgtaatcg ttgttgttaa agtagatgaa
     3961 acctttccgc agcgaagcct ggatcgcttt ggcgctggtg gggtaggcct ggatcagttt
     4021 atcgaaaaaa gtcagtgcct ggctttcctg gttaataagg atataagaca tcgctgtttc
     4081 aaagatcgca tcgtcgtggt aagaggattt agggtaatta ttgatcagaa tcttgaggac
     4141 gtcgatcttc cggttgaaat ccccttttgc gccttctgca agagctttgt gatacagggc
     4201 ataatcgagt gcggcgtctt tggccaggat gaccttatcg taataagtga tggcttcagg
     4261 gtatttttta ctgatgaaat aagcgtcgcc cagcctgaga taagcatcgg cagtgagttt
     4321 gccatcttct tcgtccggtg tatccaggta ttttttaaac tgagtgatgg cgtcgtcata
     4381 ttcctggctg ttaaaataga cataacccag gttgtaaacc gcacgggagt atagcccgga
     4441 ctgtttagcc tttttggcac ccagaaaatc aaagtaatat ttttttgcag aagggtagtt
     4501 gcctttctta tagaagatct ctccgatcca gaatagagat tcggttttta ttgtttcatc
     4561 ttttttgatg gcggcggcct tctgaaaaag atcgagtgca ccgtcgggat cgcctgcatt
     4621 ggtcagttca atggcgcggt aaaagaggat cttctggtag gcggcctgga gcttttcatt
     4681 tttttcaccg atcgattcaa tggaagccag cgcctgttta tagttccggg acgtgagata
     4741 caggcgggtc agataatcgt atgcttcgtc ggcccgtgtt gagccgggat tatcccgcag
     4801 gtaattctca agcagtgcga tcgattcgtt atagggattg aacggagact caaagccgat
     4861 cttgatataa ttaaagaggg cttcttcggc gagttctttg ttgatcccga ttttgtagga
     4921 ggccaggaaa gctccggctg catatttttt ctgccccgat tcattgtagc agattcccag
     4981 gtagtagccg gcattctgag aaagctcatc gctttcgcga acggcttgct ggaaatggtt
     5041 gacggctgtt ttataatcgc ctgatttata gttcacaaat ccggccaggt agtgatcctc
     5101 ccggctaagg gagcggcggt tggcctcttc atatatttca aagtattcgg ccgctttttc
     5161 aaaatcgttc ctgttgaagt aagcatttcc ggtcaggcgg gcaatctcgg gtttattgat
     5221 atttccttcg gaggtgatca gtgccggccc ggtggcgatg acctggtcgt agtcgcccag
     5281 gtagtgataa acctggagtt tatacagcgg ggcgattttc gcataccggg gatcatcttc
     5341 aattccggaa aaacctatca gggcctcctg gtaatttccc tggatatagt cgatatgtgc
     5401 gatataatac aatgcctggt tgaagtaggc cgacttcgga tttttcacgc gctggaaata
     5461 ggttttggcg ctggcgggtt cattcagttt caggttagca taaccgatca tgaagttcat
     5521 ttcatcgcgg gcggttttgc tcagcccggt gaggtcggct ttctgaaaag ctttgatggc
     5581 atctttgaat ttattgtcgt agaaaaagct tcgcccgagg tagaggccgg cttctccggc
     5641 gagtgcattt tccggatttt tatcgatgaa ccggtcgatc cgggcagcgg cgtcgttgtg
     5701 ggtcagttcg gcggcggtga cagcctgccg gtattcggca aaagccatca gatccgggtc
     5761 ctggcccgca tagtttccgg caatctgttc ataaatccgg cgtgcagcac ccaggtttcc
     5821 tttccggaac aaatcgtcgg cgaggcgata ttctgaagcg ggttgacggg aagctgcgtt
     5881 ttcctgtgga aatgcagggg tcatggcggt gatggccacc acggtcaaac aaaagttcct
     5941 cagcaggtta ctgtgcattt tatttaaaaa ttcaagggac tcaaaggtag aaaagcgacc
     6001 gatagggaga ggcttaaggc gattaactta ttaacaaacg atttttaatt gaacggctat
     6061 tacgggcaat tattgtataa agaaaggggg ccgttaaggc cccctgtcaa tgtctggccg
     6121 gccgtcctgt aagcaggatt ctgtgtcgtg caacggttgc acgatctctg ccatttatct
     6181 gggccggacg tcaccgcccg gctctggcag cctacccgtt ccgattccat cgttgcctcc
     6241 ggattcacgc gggcagcgtg atgagcaccg aagctgccga aggcagcgaa ggcgctgatc
     6301 ggaacatatt tggccttgca acccgtaagg tttacccgta tgccatgtca ccacagcata
     6361 ccgtgggctc ttaccccaca ttttcaccct tatcccgatc agatcgggac ggttattttc
     6421 tgtggcactt gctgtaaatc cccgcgtaag cgtggatctc cttcccgtta ggaagtacgg
     6481 cgccctgtgt tgtcccgact ttcctccccc gccaatggcg gaagcggcag agcagaccgc
     6541 cggccggcgc aaagatacaa cattttagcg ggttggtgca ccgacgacta aagctgtcgg
     6601 ttatgttgga ttcacttccg acgactaaag ccgtcggtta tgttggattc acttccgacg
     6661 actaaagccg tcggttaaag agaagtgcaa taattagtta cttttgcgac gaattttgat
     6721 ttgaaattag tataactcaa tatcgatgag ggatctgagt gtaggtaacg aacgtggcat
     6781 catcttccgt tttgctttac ctatgctgat cgggaatgtt tttcaacagt tgtacacgct
     6841 gatcaacagc atcatcgtcg ggcagttcgt ggggaaagag gccctggctg ccgtcggcgc
     6901 ttcatttccg atgatcttca ccctcatatc cctggtgatc ggcatagcca tcggggcaac
     6961 gatcatcatc agccagtatt tcggggccaa agacatgcgg aatgtcctcc gggctatcga
     7021 taccttgttg gtctttaccc tcctcgcttc tgtcgtggtc accattacgg gtattttgat
     7081 cagcaagccg attttccggc tgattgagct tcccgaagag gtctttccgc tggccgtttc
     7141 gtacttcaat atttacatgc tgggactggt tttcgctttt ggtttcaaca gtatcgccgc
     7201 gatcctgagg ggcatgggcg attctaaaac acctttgtat ttcctggtga tctccacgct
     7261 tctgaatatc gggcttgacc tgctgtttgt gctggggttc ggatggggcg tcaaaggggt
     7321 ggcctgggct acgaatatct cgcaggctgt ggcgctgctt tttgctattc tgtaccttaa
     7381 ccggacccat gatattttca aagtccgatg gcgcaagccc gacttcgacc gggctatact
     7441 caataaaagc ctgaggatcg gttttccggt ggggtttcag caggctttcg tggcattgag
     7501 catgctggcc atgtactggc tggtcaaccg tttcggcaca gatgtcgcag cggcattcag
     7561 cgtggctttc cggatcgatt cctttgcctc catgccggct atgaacttcg ccatggcgct
     7621 gtcggccttt gttggccaga acctgggagc gaggaaaccg gagcgggtga aacgcgggct
     7681 ggttgctaca ttctacatga cagcaattat ttcggttacc atctcgttgg tgggaatatt
     7741 ttttgccggg ccattgatgc ggttattcac tacagacgcg gccgtcgttg aggtagggaa
     7801 ggattacctt cagatcgtga ccggattcta tattgtcttc tcttcgatgt ttgtggtggg
     7861 tggcgtaatg cgcggcgccg gcgacacctt catccccatg ctgatcactt tctttgcctt
     7921 gtgggttgtc cgtattcccg tgggatattt cttgtcggag aagatgggat accacggcat
     7981 atggtggtgc attccgatag cctgggtggt ggggctgggg ctttcgtttg cctattattc
     8041 caccgggagg tggcgaaaaa aagtggtttt gttagtataa taatttttta tggacttgct
     8101 tttccctccg gaataacgta tctttaatgc gtcaatttat ccccccgcat tattaaccag
     8161 ttattctgaa acgcatgaaa agaatttttt ttaaaaccag agccatagca ggattgctta
     8221 tggtgttatt ttttattaaa acccttatcg tgaactccca gtcgattgag atttaccaaa
     8281 ccttcatatc agatgctgaa atacagcctt tcacgcccga agacacaatt tatggcataa
     8341 ccgtcagcgg tttggtgaat ctgaaaagtg ataccagttt ggtcaggata atcctatcag
     8401 atataaacgg tactaaatgg atgattcatg aatcctatcc gttgatcgcc agtattaaag
     8461 aatttaacat ggaaaacgat tgtgacgaga cttgttatct cgatgaaacg cttcctgttt
     8521 cgatcaccat tcaaataatt gatgctgaac tatatatttc gacgtttagt ttaagcacca
     8581 tcccgagtga aaacccttcc ctgcttcaat atcaggcaaa aagaagtaaa gacctggaaa
     8641 aagttcagca aatgaatatc aatatacaag caacgggatg gaattggact gctgatacta
     8701 ccaaccttgt aaagatgttt tataaagaaa agattaaaaa ttttgatgaa aagttcaacc
     8761 ttttaggttt agaattttac tctggcggga tttataaatc gatta
//
LOCUS       DAOPNH010000138         8798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_8793_length_8798_cov_7.227039, whole genome shotgun sequence.
ACCESSION   DAOPNH010000138 DAOPNH010000000
VERSION     DAOPNH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8793_length_8798_cov_7.227039"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1870
                     /locus_tag="PKZ74_08420"
     CDS             47..1870
                     /locus_tag="PKZ74_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92821.1"
                     /translation="MSIPEKFMSQTSYKYPEREFSKKKITKEGMQRLYRLFRFVGPQK
                     YTFIAGMAFLVLSSLTTLVFPMLIGELLDAASSSTLEKINELGMILIGVFLVSAVFSY
                     FRIYLFAVVTQKTLALLRQTTYNHLIKLPMAFFSSRRVGELNSRISADISLLQETFTT
                     TLAQFVRQVITIVGGIVLLTVISPRLTVFMLALIPVAAIVARIFGTYIRKLSKKAQTV
                     VAESNTIVEETLQAVASVKAFANEAFEIMRYKKKTDEVIAISLKGAKWRGLFISFIMF
                     ALFGSIIGVIWYGVYLIHQGAGLSTGDLLKFVLYSVFIGGSISGVADLYSDIQKSIGA
                     TENLLDILDETPEDLLSETTGSATGITGDVRFENVSFSYPTRKDVAVLKDITFSVRHG
                     GQVALVGPSGAGKSTISALLLRFYDPTGGRILIDGKDARDYDLTALRGNMAIVPQEVL
                     LFGGTIRENIEYGKPGATKEEILHAAELANAMEFIGKFPEGLETLVGERGVQLSGGQR
                     QRIAIARAILKNPAILILDEATSSLDAENERLVQEALEKLMEGRTSFVIAHRLSTIHK
                     ADQIVVIDQGEVKEMGTHEELLAREGGIYQQLTRLQINVMG"
     gene            complement(2098..2601)
                     /locus_tag="PKZ74_08425"
     CDS             complement(2098..2601)
                     /locus_tag="PKZ74_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92822.1"
                     /translation="MKDKKINTLVCTAQWLAALTGSIYGLVLAIFGYLSGWGPGGSMN
                     GPFWRVLLSAAISILAGAFIGTFVVRKLARLLFEKPFSKLESGLRAFALVLAGSFPAF
                     VSSWLTGYIMGKLTGTIEGLDWITVLVYTPLMSFIYGIPVCFGTDLLFGIFVFFYLKD
                     VDDLRRV"
     gene            complement(2621..3781)
                     /locus_tag="PKZ74_08430"
     CDS             complement(2621..3781)
                     /locus_tag="PKZ74_08430"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPM92823.1"
                     /translation="MKKVIVRTTLFIIIIHTGLFGQNSIRFTFSAVEGSDYFRLDSLR
                     VRNMTQDCDTVLIYPDTVLVINYVGINDSQNSAGGFNMIRNFPNPVTSLSKINLEIPE
                     KDYVNLTLADAAGRILYSASLTLGPGIHRFLFTPPGNGLFIFNASWRGNNCSARILAS
                     GVSQGNEFSLKYMCTSGSSNTLKTRRSIAEYLYNPGDELLLTGYGDGLESGFADSPET
                     DQEYIFQFAANIPCPCLDSLLYDGQWYHTIQVFSQCWMKENLNAGIMIPSSQAQTNNG
                     IIEKYCMINNESYCDILGGLYFWNETMQHVLETGGRGICPEGFHVPTDLEWQILEGAV
                     DSELEIGAPGWNASGWRGTDAGGNLKQTGTDLWEPPNTGGTGAAFSVRCIRN"
     gene            complement(3889..4536)
                     /locus_tag="PKZ74_08435"
     CDS             complement(3889..4536)
                     /locus_tag="PKZ74_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92824.1"
                     /translation="MKRLFNILIIIVVYMIFCGKSCDDDGERINRQSHRASLAKDSIL
                     HDFELDFLTEEARYAAGVNAMQKLNDLADYMEIYADRSLEPAFREKAGEMINKMFRSP
                     DNHLFLGPVRKEKMAQITPEEFLKKGFGDDYIRVKINFDSVKVIEPLVLKDEGNYSGK
                     MSAYQSMIGFTANDSLVLPARRVTIDFFSTRQVKTFGSDSIEVRVVRLGDIYLEK"
     gene            complement(4537..5463)
                     /locus_tag="PKZ74_08440"
     CDS             complement(4537..5463)
                     /locus_tag="PKZ74_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92825.1"
                     /translation="MQQKENTDFKSLKTRIVATMQQSYPGLSSDISEWKGQDIVNFQE
                     ELLQKVNAHISEKWFYMHMKSGKSTLPRIDVLNLLSRYAGYADWNDFVYRNGGQKPKP
                     AVSSGNRYFIIIPLLVIAILGIFYLVNKLVSNRNYTFCFYDATTREQITHADIEVKII
                     LENESPITYLCSSDGCFTLKTDRSFIKMVVNSPYYRADTINRTLKKFNPQEIIGLQAN
                     DYALMLHYFSEMNTEDWQKRRLKLDEMFDESAMIYQVVNDKSGTGMELYSKWEFIDKL
                     TMPSHSLKNIEILDTKYIGDRIVVLRFRISDK"
     gene            5669..6037
                     /locus_tag="PKZ74_08445"
     CDS             5669..6037
                     /locus_tag="PKZ74_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92826.1"
                     /translation="MKKQILRWAPRIIAILAVLFMMIFSMDCFGPGYSLKEQLICFVM
                     HNIPAFIVIAALVIAWKWELIGGILFVLIFLAAGIFFHSFGRNPASLIVITPFLVAGV
                     LFMLDYFLMKDKSEMESHGN"
     gene            6114..6923
                     /locus_tag="PKZ74_08450"
     CDS             6114..6923
                     /locus_tag="PKZ74_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92827.1"
                     /translation="MQNHFTTHFEAIHSKITLILFIIAFLFITASPSIGIADNPLAMV
                     LPIAGFKYFFFICKAEPGLIIQKRARWISCLILLIAMSGCKPGSVLIRGDDCRMNGSF
                     EHVKNGFPVNWCYYAMETVPDGDFDILIDSVQSKEGKRSLKFQVRTCSSIGGWYSPGF
                     FEEFKVLPGQTYKISFCVLNQGCDFKVGVSTGMKGNPGPSETILRTNGKYSDWHYFEY
                     SFTIPLVNDNIRFEANILSPGTILFDDIRIEGLTDKDELTLYPYRGSEKCK"
     gene            6955..7359
                     /locus_tag="PKZ74_08455"
     CDS             6955..7359
                     /locus_tag="PKZ74_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92828.1"
                     /translation="MIEHVHKHITTELQQNTKTDTIFILTAILLNLIALAVNSGLVED
                     SRTDNSLLIVMYLFVFLIIVVNIVVIIGIMKGKQTRMKLVDGLMRMYKDQGVDKYYDE
                     SLLSNYSLRYNLFIMVVVSTGIIATVVPFVVR"
     gene            7396..>8798
                     /locus_tag="PKZ74_08460"
     CDS             7396..>8798
                     /locus_tag="PKZ74_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPM92829.1"
                     /translation="MRWSVNLPETPSGLIEMESQFRIFGKKHPMGILSALSKPYSRHI
                     AGKIRHDSLHAVECQLKMMNMLVERGRTTIYGKARHFNEIVSYEDFRRHIPLTDYEDI
                     RPLIERISTGEKDILWPGRPIYFAKTSGTTSGTKYIPVTRDSISNHINTARNALLVYM
                     DRTKNFAFADHSMIFLQGSPVLDKKGVIPAGRLSGIVAHHVPGYLQKNRMPSWKTNCI
                     DDWETKVDAIVEETLKKRMSLISGIPSWLRMYFEKLVDKAGKPVGEIFPDFSLLVYGG
                     LNYEPYREAFKKLIGRPVGTLELYPASEGFLAFQDEGPGEGLLLNVNSGIFFEFIPVN
                     KMHNDDPERIPLEGVEKNTDYAVVLSNNAGLWGYNLGDTVRFVSINPYRLVVTGRISQ
                     FISAFGEHVIASEVEGALAEVCAATGTQVAEFTVAPVVNPPAGLPHHEWLIEFITPVE
                     TLQARDRPRWPRCRQGRA"
BASE COUNT         2248 a         2065 c         2160 g         2325 t
ORIGIN      
        1 ggcataactc attctaagca gttttgcttt cgcaaacctg caaagaatga gtatacctga
       61 aaaatttatg tcgcagacgt catacaaata tcccgaaagg gaattttcca aaaagaagat
      121 cacaaaggaa gggatgcagc ggttataccg gctgttccgc tttgtgggcc cgcagaaata
      181 cacctttatt gcgggaatgg ccttcctggt gctttccagc ctgaccacgc tggtattccc
      241 gatgctgatc ggggaattgc tggatgccgc atccagttcc accctcgaaa aaatcaacga
      301 gctggggatg atcctgatcg gtgttttcct ggtaagcgcc gttttctcct atttccggat
      361 ctacctgttt gccgtcgtta cccagaaaac cctggcgctg ctccggcaga ccacctacaa
      421 ccatctcatc aagctgccga tggcattttt ttcgagccgg cgggtcgggg aattgaacag
      481 ccgcatctcg gcggatattt cgctgctgca ggagacattc accaccaccc tggcgcagtt
      541 tgtcaggcag gtgatcacca ttgtcggggg catagtgctg ctgacggtca tttcgcccag
      601 gctgacggtg ttcatgctgg cgctgatccc tgtggcggct attgttgcca ggatcttcgg
      661 gacctacatc cggaagctgt cgaagaaagc acagacggtg gtagccgaat cgaacacgat
      721 cgttgaagag acgctgcagg cggttgccag tgtcaaggct tttgccaacg aggctttcga
      781 gatcatgaga tacaagaaga agaccgacga agtgatcgcc atttcgctga aaggggccaa
      841 atggcgcggg cttttcatct ctttcatcat gtttgccctg ttcggatcga tcatcggggt
      901 gatctggtac ggcgtttacc tgatccacca gggggcgggg ctgtcgacgg gcgatctgct
      961 gaagttcgtg ctttattcgg tgttcatcgg cggctcgatc agcggggtgg ccgacctgta
     1021 ttccgatatc cagaagtcga tcggggcgac ggagaatttg ctcgacatcc ttgatgagac
     1081 gccggaagat ttactttcgg agactacggg aagcgcaacg ggaattaccg gggatgtccg
     1141 gtttgagaat gtatcctttt cctacccaac ccgcaaggat gtcgctgtac tgaaagacat
     1201 taccttcagt gtgcgccacg gcgggcaggt agcgctggtt ggcccgtcgg gggcgggcaa
     1261 gagcaccatc agcgcgttgt tgctccggtt ttatgatccc acgggcggcc gcatcctgat
     1321 cgacgggaaa gatgcccggg attacgatct cacggcatta cgggggaata tggcgatagt
     1381 gccgcaggag gtattgctgt tcggtggcac gatccgggaa aatatcgaat acggcaaacc
     1441 cggcgccacg aaggaagaga ttttgcatgc ggcggagctg gccaatgcga tggagttcat
     1501 cggcaagttc ccggaagggt tggaaaccct cgtcggggag cgcggcgtgc aactctctgg
     1561 cggtcagcgg caacgcatag ccattgcccg tgccatattg aaaaatcctg ccatcctgat
     1621 cctggatgaa gcaaccagtt cgctcgacgc ggagaatgag cggctggtgc aggaggcgct
     1681 ggagaagctg atggaagggc gcacctcgtt cgtgatcgcc caccggcttt cgaccatcca
     1741 caaagccgac cagatcgtgg tgatcgacca gggtgaagtg aaagagatgg ggacacacga
     1801 ggagttgctg gcccgcgaag gcgggatcta ccagcagctt acacggttgc agatcaatgt
     1861 gatgggatga gggagggcag gcggcggtca gaccttcacg atattgccgg tcagcagccg
     1921 acggcctgtc ctgagttcgc cagtggtgga cgaagtttcg ttagttgcta ttagtagata
     1981 ccggagggcc ggttttcggc gttccaggat catgctaccg tgaaccaaat tccgactcaa
     2041 atttactgcc tgataaagga aaagtcaata catatttcat cggatttttt atcagttcta
     2101 tactcttcta agatcatcaa cgtctttcaa ataaaaaaat acaaaaatgc caaacaaaag
     2161 atccgttcca aaacataccg ggatgccata gataaaagac atgagcggag tgtaaaccaa
     2221 gactgtgatc caatcgagcc cctcaatggt acccgtcaat ttccccatta tgtacccggt
     2281 taaccagctg gaaacaaaag cagggaaact tcctgcaagg accagggcaa aggctctaag
     2341 tcccgattcc aactttgaaa atggtttttc gaatagtaac cgtgccagtt tcctgacgac
     2401 aaatgtaccg atgaaagctc ctgccaggat cgatatggct gccgacagca ggactctcca
     2461 gaatggcccg ttcatgcttc ctcccgggcc ccaacccgac agataaccaa aaatggccag
     2521 caccaggcca tagatgctgc cggtcaatgc tgcaagccat tgggctgtgc acaccagggt
     2581 attaattttt ttatccttca cattttcaaa tttagaatac ctaattcctg atacacctca
     2641 ccgaaaacgc tgcccccgtc ccgccggtat tcggtggttc ccagagatct gtcccggttt
     2701 gtttaagatt acccccggca tctgtccctc gccagccact tgcattccag ccgggtgccc
     2761 cgatctcaag ttcgctgtcg acggctcctt ccaggatctg ccactcgagg tctgtcggta
     2821 cgtgaaatcc ttcagggcaa atacccctgc ctcccgtttc aagaacatgc tgcatagttt
     2881 cattccagaa ataaaggccg ccaaggatgt cacaatatga ctcgttattg atcatgcaat
     2941 acttttcgat gatgccgttg ttcgtctggg cctgggaaga agggatcata atgccggcgt
     3001 tgaggttctc cttcatccag cactggctga atacctggat ggtgtgatac cactgcccgt
     3061 catagagcag ggaatcgagg caggggcagg gaatgttcgc cgcaaactgg aaaatatatt
     3121 cctggtcggt ttcgggcgaa tctgcgaacc ctgattcaag accatcgcca tatccggtaa
     3181 gcaggagctc atcgccggga ttataaagat attctgcgat tgacctgcga gttttcaacg
     3241 tattcgaaga gcctgacgta cacatgtatt tcagtgaaaa ttcgtttccc tgactgacac
     3301 cggaagccag gatccgtgca gagcaattgt tccctctcca ggaagcattg aagataaaaa
     3361 gcccgttccc gggtggcgtg aagagaaatc tatggattcc gggacccagt gtcagtgagg
     3421 ctgaatacag tatgcgcccg gcggcatctg ccaatgtcag gtttacgtaa tctttttcag
     3481 gtatttcaag attaatctta gacagactgg tgacagggtt cgggaagttc cgaatcatgt
     3541 tgaaaccgcc agcactattt tggctgtcgt tgattcccac ataattaata accagaactg
     3601 tatcaggata aatcagcaca gtatcgcaat cctgagtcat attccttacc ctcaggctat
     3661 ccagccggaa atagtctgat ccctcaacag cggaaaatgt gaatcggatt gaattctgcc
     3721 caaaaaggcc ggtatggatg atgattataa acaaggtggt acgaacaatg acttttttca
     3781 tatgacttga attaaaaaca agcctcaaaa atataactgt cgttaccgtt tcttctgtaa
     3841 gtcgcttgta aaggatttgt aaattatttt gcaaattgag atggagtctc acttttccag
     3901 gtaaatatcc cccagtctga ccaccctcac ttctatcgag tcgcttccga aggttttaac
     3961 ctggcgggtt gaaaagaagt caattgtcac ccgcctggcc ggcagaacca ggctgtcatt
     4021 agcggtaaat ccaatcattg actggtaagc cgacatcttg ccagaataat ttccttcatc
     4081 ttttaggacc agcggctcaa taaccttgac agagtcaaaa ttaatcttca cccggatata
     4141 atcatctccg aatccttttt tcaaaaattc ctccggggtt atctgtgcca tcttctcctt
     4201 cctgaccggt ccaagaaaca ggtggttatc cggtgaacga aacatcttgt tgatcatttc
     4261 acccgctttc tccctgaatg cgggttccag cgaccgatcg gcatagattt ccatataatc
     4321 ggcaaggtcg ttcagctttt gcattgcatt gacaccggcg gcataacggg cttcttctgt
     4381 taaaaaatcc aattcaaaat catggagaat gctgtccttt gccaaagatg cccggtgact
     4441 ttgcctgtta atcctttcgc cgtcgtcatc gcacgacttc ccgcagaaga tcatgtatac
     4501 tacaatgatg atcaatatgt taaacaatct tttcatttat ttatccgata ttctgaaccg
     4561 tagtacaaca atacggtcac caatgtactt cgtatctaag atttcgatat ttttcaacga
     4621 atgggaaggc atcgtcagtt tatctataaa ctcccatttg ctgtatagtt ccatcccggt
     4681 gccgctttta tcgttcacga cctggtagat catcgcgctt tcatcaaaca tttcatccag
     4741 tttcagtcgt cttttctgcc agtcctccgt attcatctcc gaaaaataat gcagcatcaa
     4801 tgcataatcg ttggcctgga ggccgatgat ctcctgcggg ttgaacttct tcagcgtcct
     4861 gtttatggta tcggcacgat agtagggtga atttacgacc attttgatga agctccggtc
     4921 ggttttcagt gtgaaacacc catcggaact gcacaggtat gttattggcg attcattttc
     4981 caggatgatt ttcacctcga tatctgcatg ggttatttgt tccctggtcg tggcatcata
     5041 gaagcagaaa gtataattcc tgttgctgac cagcttattg accaggtaga aaatgccgag
     5101 gattgcaatg acaagcaggg gaattattat aaaataacgg ttaccgctgc ttacggctgg
     5161 ttttggtttt tggccgccat tcctgtatac aaaatcgttc cagtcggcat acccggcgta
     5221 cctgctcaac aggttaagca catcgattcg cggcagggta cttttgcccg atttcatatg
     5281 catgtagaac catttttcgc tgatgtgggc attcactttc tgaagcagtt cttcctggaa
     5341 attgacgata tcctggcctt tccattccga aatatcggaa cttaaccccg gataagattg
     5401 ttgcatggtt gccacgatcc tggtcttaag tgatttaaag tcggtatttt ccttctgttg
     5461 caaagatgaa acggattaat gaaacgtgga tgaagaacgc ttgtaaaagg atttacaatg
     5521 gttttacaac aggaattcca tggtatctcc ctgaaaacga tgtaatttta tatcaatgat
     5581 cagaattatt ttggttaact ttaggtaaag ttacgacgtc aattgtgtca aatcaatttt
     5641 tcaggccaga atctttaaaa ataataagat gaaaaagcaa attctacgct gggccccacg
     5701 gatcatcgcc atcctggcgg tccttttcat gatgatcttt tcgatggatt gttttggtcc
     5761 cggatattcc ttgaaggaac aattgatctg ctttgtgatg cacaacatac cggctttcat
     5821 tgtcattgct gctctggtga ttgcctggaa gtgggagctg atcgggggga tattgtttgt
     5881 gcttatcttt ctggcagcgg gaatcttttt ccacagtttt ggtcgcaatc cagcgtcact
     5941 catagtcata actccatttt tggtggcggg ggtgttattt atgctggatt attttctgat
     6001 gaaagataaa agtgagatgg agtctcatgg caattgagat ggagtctcat accaaacgag
     6061 acgccatctc gaaaaattga gatggagtct ctgtgattta aatagttatt agtatgcaaa
     6121 atcattttac cacacatttt gaggccattc attcaaagat tactctgatc ctttttataa
     6181 ttgcttttct gtttatcaca gcctcgcctt ctattggcat agccgacaac ccgctggcca
     6241 tggttttacc tatagcagga ttcaaatatt ttttctttat ctgtaaggca gaacctggcc
     6301 tgatcatcca aaaaagggcc cgctggatat cgtgtctaat attactgatt gcaatgtcag
     6361 gttgcaaacc cggaagtgtt ttgataagag gagatgattg cagaatgaat gggagttttg
     6421 aacatgtaaa aaacggtttc ccggtaaatt ggtgttatta cgcaatggaa acggtaccgg
     6481 atggtgattt tgatattctt atcgattctg ttcagtcaaa agaaggtaaa agatctctga
     6541 aatttcaggt gcgaacctgt tcgtccattg gcgggtggta ttctcccgga ttctttgagg
     6601 aatttaaagt tttgcccggc caaacttata aaataagctt ctgtgtatta aatcagggct
     6661 gtgattttaa agtcggcgtc agtactggca tgaaaggaaa tccaggccca agtgaaacca
     6721 ttcttagaac caatggaaag tattcagatt ggcattattt tgaatattct ttcaccatcc
     6781 cactggtgaa tgacaatata aggtttgaag caaatatatt atcgcctggc acgatcttgt
     6841 ttgatgatat tagaattgaa gggctgaccg ataaagatga attgaccctg tatccgtaca
     6901 ggggatccga aaaatgtaaa tagttaaagt tataaacttt aaaatcaatg tattatgata
     6961 gagcacgtgc acaaacacat tacaaccgag ttacagcaga acacgaagac cgatacgatc
     7021 ttcatcctga cggcgatcct gctcaacctg atcgccctgg ctgttaattc agggctggta
     7081 gaagactcaa ggaccgacaa ctcactcctg atcgttatgt atttatttgt attcctgatc
     7141 attgtggtga atattgtagt aataatcggc ataatgaaag gcaagcagac ccggatgaaa
     7201 ctggtggatg ggctgatgcg gatgtataaa gaccagggtg ttgataaata ctatgatgaa
     7261 tcgttattga gcaattatag cctcaggtat aatctattta tcatggttgt cgtgagtacg
     7321 ggcattatag ccacggtagt gccgtttgtg gtgaggtgat tttgtgttgc ttaaacgaga
     7381 ctccatctcg agtaaatgag atggagtgtc aatcttccgg agactccatc tgggctcatc
     7441 gagatggagt ctcaatttcg tatctttggg aaaaaacacc cgatgggaat tctctctgct
     7501 ctcagcaaac cttattcacg gcatattgcc ggaaaaatcc gtcatgattc cctgcatgca
     7561 gtggagtgcc agttgaaaat gatgaatatg ctggttgaac gtggtcgcac gacaatctat
     7621 ggtaaggccc ggcatttcaa tgagatcgta agctatgaag actttcgcag gcacattccg
     7681 ctgactgatt acgaagatat ccgaccattg atcgaaagga tatccaccgg ggaaaaagac
     7741 attctctggc ccgggcggcc gatttatttt gcgaagactt ccggcaccac atcaggcaca
     7801 aagtatatcc ctgtcacacg agactccatc tctaatcata tcaacaccgc ccgcaatgcc
     7861 ctgctggtct acatggaccg gacaaaaaac tttgcctttg ccgaccacag tatgattttt
     7921 ctgcagggca gcccggtgct ggataaaaag ggtgtgatcc cggcgggccg gctttcgggg
     7981 atcgtggccc accatgtgcc cggttacctt cagaagaacc gcatgccctc ctggaaaacc
     8041 aactgcatcg atgattggga gacgaaagtc gatgcaatcg ttgaggaaac actgaagaag
     8101 aggatgtcgc tcatcagcgg catcccatcc tggttgagaa tgtatttcga aaaactcgtt
     8161 gacaaggccg gcaaaccggt cggggagatt ttccccgatt tctccttgct cgtttatggc
     8221 gggctgaact atgagcccta ccgggaggcc tttaaaaaac tgatcgggcg cccggtcggc
     8281 acgctggaac tctaccccgc atcggaagga ttcctggctt tccaggacga agggccgggt
     8341 gaaggactgc tcctgaatgt caacagcggc atcttcttcg aatttatccc cgtcaacaag
     8401 atgcataatg atgatccgga gcgcatcccg ctggaagggg ttgaaaaaaa caccgattat
     8461 gcggtggttc tcagcaacaa tgccggcctc tggggatata acctcggcga tacggtacgc
     8521 ttcgtctcga ttaatccgta ccgattggta gttaccggcc gtatttcgca gttcatatcc
     8581 gcattcggtg aacatgtcat cgcctcggag gtggaagggg ccctggcaga ggtctgcgcg
     8641 gccacaggta cacaggtggc cgaatttacc gtggcgcctg tggtgaaccc gccggcggga
     8701 ttaccccacc acgaatggtt gatcgaattt ataaccccgg tggaaacgtt gcaagcacga
     8761 gatcgccctc gatggccccg atgccggcaa gggagagc
//
LOCUS       DAOPNH010000139         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9079_length_8612_cov_5.795606, whole genome shotgun sequence.
ACCESSION   DAOPNH010000139 DAOPNH010000000
VERSION     DAOPNH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9079_length_8612_cov_5.795606"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKZ74_08465"
     CDS             <1..1659
                     /locus_tag="PKZ74_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPM92830.1"
                     /translation="ETVAYCKLLDEASESIYYTTFGSSPQGRDLPLLIIDSDGLNDPA
                     SVNNAGKLVVLIQAGIHPGEIDGKDAGFMFFRDLVILKKLGKLPENLTFLFIPVMNVD
                     GHERFGPYSRINQDGPEQMGWRTTAQNLNLNRDFLKADAPEMQAWLRLYQQWKPGFVA
                     DIHVTDGADYQYVMTYGLETHGNMDEGLTAWTQGSFIPEMESWMEKAGYPAGPYVMFR
                     RWHDPRSGLRSGAAGPRYSTGYMATRNRICLLVENHSLKNYKTRVSSTYELLKFLTDY
                     LDKNAFKIKILNAAADKYAVSDAFRARPFALSYKAGPDSIFVDFKGVEYDVEKSDLTG
                     GDWFRYHPDKPVTFKVPLFHQQIPAATVLIPEAYIIPPEWNEIIDRLELHGVQYYRLE
                     KPADIKIESYRFTKTDFSKTSFEGRIQVTPTFEAFNEVRQFPAGSVVIPTDQVAVRAI
                     VHMLEPGSPDSFLQWGFLNGIFELKEYFETYVMEEYARKMIAENPALKEEFNKWKAEN
                     PEASQSQWSQLEWFYFRSPYLDRKWNVYPIGRILEKSKLEELGL"
     gene            complement(1792..2295)
                     /locus_tag="PKZ74_08470"
     CDS             complement(1792..2295)
                     /locus_tag="PKZ74_08470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPM92831.1"
                     /translation="MTTFYPSPIGMIRLEISGRGLAGLEFSDLSETPSRCIETPSHST
                     ETPSHSNQMESRIIRQLDEYFSRKRKVFDLELDLQGTDFQKQVWMELLKIPYGRTITY
                     KELSIRLGNPGVIRAAGAANGANPVSIIVPCHRVIGSDGSLTGYAGGLWRKKWLLDFE
                     SDEKFLF"
     gene            2432..4654
                     /locus_tag="PKZ74_08475"
     CDS             2432..4654
                     /locus_tag="PKZ74_08475"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM92832.1"
                     /translation="MKTITPILIILFHLSATDWACSQWTCQNPLPQVNTLNDVFFIDP
                     ETGWVVGEAGTIMKSTDGGQTYDLLNFQTHLNFSCVKFFDLNNGLIAGDSGIILVSDE
                     GGSNWNRVETGFSADITDIYIINNCISWLSCTNGTIMKSDDFGGSWSIAYDNPLLEFN
                     SLSFADEMHGWAAGKDNTGFPAIVRTCDGGLSWIELDPPFEDQIFDICFADTLVGYAS
                     LRDIDYETRLAKTTDGGLSWLFLLDDVFYGEFYDIEFIDAEHGWIAGSSASIPSAWGI
                     FFYTSDGGTTWNPGDITHDYNHRAFNSVYFINEDTGWMVGSLGIILHSNDGGIHWSKS
                     NTGFTGDCHFEDVFFVDDQKGWAVGAGFYPYESGILHTTDGGNSWTPQPALEHMLNSV
                     FFINPLEGWIVGGRSLQEYTTAIYHTNSGGDQWDKQFSENINDGDFSDVFFKDAMHGW
                     AVGSGNNYYPPMNNSLFFYTEDGGENWIDQSAMISHALSSICFTDDETGFISGHKIIL
                     RSINGGKSWTEVWNGSHYLRNIFFTDINHGWAIGDSATRYPYRDVIMRTTDGGTTWEE
                     QFPGFGSMKKQLFFTDNSHGWIARDEGDILYTIDGGITWQHMFVNYNWSFGGIFFTDI
                     DNGWVVGGNKTIFHINNGGTVGITEPSAAGGHSPVSAYPNPTTGIVEFRLSNFDCRWI
                     SLKIYDLQGQEVATVMDGRWYGSKGVRWDASGLPAGVYFYQLRTKGEGQGAAGKIVKL
                     "
     gene            4795..5277
                     /locus_tag="PKZ74_08480"
     CDS             4795..5277
                     /locus_tag="PKZ74_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM92833.1"
                     /translation="MKKALLLISLIFISVRIFSQVEEINIYDPDANAREDIANAVRQA
                     DAGNKHVLLQIGGNWCPWCVRLHHLESSDPQIDSLLKADYIRVMVNVPKEKDKRDYEL
                     LKTLGNPQRFGFPVLVFLNRKGERIHTQDSWYLEQDKAYDREKVIHLFKMWNVVAAGN
                     "
     gene            complement(5274..6863)
                     /locus_tag="PKZ74_08485"
     CDS             complement(5274..6863)
                     /locus_tag="PKZ74_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPM92834.1"
                     /translation="MKMRFPHTYVIIFSLIILAAILTWIFPGGDYVEQVKTVDGKEVK
                     ELVFIEQDNAPQTWQVFGTMFKGFVRQAGIIVLILMIGGAFWIMNTTKALDMGILSFI
                     HFTRSLEKFRFFKLIGVDNVVIVLIMLMFSLFGAIFGMSEESIAFVIILVPLAISMGY
                     DSIVGVAMCYLAAHIGFAAAMLNPFTIGIAQGIAGLPLFSGLEYRFFCFIIINIIGIG
                     FVLWYARRIKKNPQRSPVHDIDVYWRDKAAGTTERMEHHTPVSAWVSFALTLAVLIAF
                     SVYYPMTEIKIGNSAAMIIPAIPVATVLFAFLGLISLQKTVHYFILTLLLFTIVFLVI
                     GVMGYSWYIMEIAALFFAMGIASGVAMGYGGNTQVKLFLDGVRDIVSAALVVGLAGGI
                     IVVLEDGKVIDTILHRLASSLHQAGQIQAVGTMYVIQTIINLVIPSGSAKAALTMPIM
                     APFSDLINLSRQATVMAFQFGDGFTNMITPTSGVLIGALGVARIPYDRWFRWVWPIIL
                     IFFILGFLLLIPTVTMELNGF"
     gene            complement(6993..>8612)
                     /locus_tag="PKZ74_08490"
     CDS             complement(6993..>8612)
                     /locus_tag="PKZ74_08490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPM92835.1"
                     /translation="CLVTKTSLMKKLYILLIVLIITSPNDLSAQVDKSDWVGTVPDGC
                     TSITCGKRATIDGSVITSHTDDSHRTRSSIFIQPALDHPEGSRCDITQRVPCDTFAMP
                     LYVYKKIGTIPQVKHTFAYVNSAYPCINEHQLAIGESTFGGRESMQSGKGLIDCTMLC
                     QLMVERCTTAREAIAMAGELTAKYGYNDAGECLTIADKKEVWHLEILGPGKDQVGSVW
                     AAQRVPDDHVSVNANASRIRKINLKDKEHFMASENIFQVAIDSGWCTTKDDFEFCYAY
                     APESRTTLASRRREWRVLDLVAPSLKLDPNAENYPFSVKPDSLVTLPQLVSIFKDYYE
                     GTPFDMTKDMTVTGDDGKSIISPLANPHMPYDANKLFKINGGWGWLGERTIARWYTMY
                     ATIIQCREWLPDPVGGVVWLAMDNVATSVYIPVYCGVTDLPEPYKVDGRIEGFSNKSA
                     WWAFNRLGTLTAQRWGDMRHDVDAIWNPIQQELFDRQKSVELQAVQMLKEDPGKAKAF
                     LTSYTGMWGSYVVDKAWKLGDYLWTKYDEKF"
BASE COUNT         2235 a         2083 c         2177 g         2117 t
ORIGIN      
        1 gaaaccgtgg cttactgcaa attgctcgac gaagcctcgg agtctattta ttatacaaca
       61 ttcggaagct caccccaggg aagagacctc ccgctgctga tcatcgacag tgacgggctg
      121 aatgacccgg cctcggtcaa taatgccggc aaactcgttg ttttgatcca ggccgggatc
      181 cacccgggag aaatcgatgg caaagacgcc gggttcatgt tcttccgcga ccttgtcata
      241 ctgaaaaagc tcggcaaatt acctgaaaac ctcacgtttc tttttatccc cgtcatgaat
      301 gtcgacggcc atgagcgttt cggaccttac agccggatca accaggatgg cccggagcag
      361 atgggatggc gcacaacagc ccagaacctc aacctgaacc gcgattttct taaagctgac
      421 gctcccgaga tgcaggcctg gctcaggctt taccagcaat ggaaaccggg ctttgttgcc
      481 gacatccatg ttaccgatgg cgctgattat cagtatgtga tgacctatgg gttggaaacc
      541 cacgggaata tggacgaggg cctgacagcc tggacccagg ggtcatttat cccggagatg
      601 gagtcatgga tggaaaaagc cggctatcct gccggccctt acgttatgtt ccgccgctgg
      661 cacgatcccc gaagcgggct gaggagcgga gccgcaggtc cgcgctattc aaccggctac
      721 atggcaaccc ggaaccggat ctgcctgctg gtggagaatc attctcttaa gaattataaa
      781 acacgggtaa gcagcactta cgaattattg aaatttctta cggattatct tgataaaaac
      841 gcgtttaaga tcaaaatcct gaatgccgct gcagataaat atgctgtatc tgacgctttt
      901 cgcgccaggc ctttcgcttt aagctacaaa gcaggccccg acagcatctt tgtcgatttt
      961 aaaggggtgg aatacgatgt tgagaaaagc gacctgaccg gcggtgactg gttccgatat
     1021 catcccgaca aaccggtaac tttcaaggtg cccctgttcc atcagcagat cccggcagct
     1081 acggtcttaa ttcctgaggc ttacatcatt ccgcccgaat ggaatgagat tatcgaccgt
     1141 ctggaactgc atggagtgca gtattaccgc ctggagaaac ctgcagatat caaaattgaa
     1201 agttatcgat ttacaaagac cgatttcagt aagacctcat tcgaagggcg gatccaggtg
     1261 acgcccacct tcgaagcttt caatgaagtg cggcaattcc cggccggctc ggtggttatc
     1321 ccgacagacc aggtagctgt gagagccatt gtccatatgc tcgagcccgg ctcgcccgac
     1381 tcctttctcc agtggggttt ccttaacggg atttttgaat tgaaagaata tttcgagacc
     1441 tatgtgatgg aagaatatgc acggaagatg atcgcggaga atcctgcgtt gaaggaagaa
     1501 ttcaataaat ggaaggctga gaaccccgaa gcctcccaga gccagtggtc gcagctggag
     1561 tggttctatt tccgctctcc ctatctcgac aggaaatgga atgtttatcc gattggcaga
     1621 atcctggaga aaagcaagct ggaggagtta ggactgtagg atggtaggac cgtaggactg
     1681 aaggacgaag gactgtaaga ctgttggacg gcaagactgt aggactttaa gaatccgtgt
     1741 aaatccgtta tatctgtgtc atctgtgttc tattaatagc ctaatgaaca ttcagaacaa
     1801 aaatttttca tcggattcga aatcaagaag ccattttttc ctccacaatc ccccggcata
     1861 gcctgtcaac gatccatcgg aaccgatcac ccggtggcag ggaacgatga tgctgaccgg
     1921 gttggcgccg ttggctgccc ctgcggcgcg gataacgccg ggattgccca gcctgatcga
     1981 gagttctttg taggttatcg ttcttccata gggaatcttc agcaattcca tccatacctg
     2041 cttctggaag tcagtcccct gcaggtcgag ttccaggtcg aacactttgc gcttgcggga
     2101 gaaatattcg tcgagctggc ggatgattcg agactccatc tggtttgagt gagatggcgt
     2161 ctcggttgag tgagatggag tctcgatgca acgagatgga gtctcactta aatcactgaa
     2221 ttccaggccc gccagccccc tcccggaaat ctcaagccgg atcatgccta tcggcgatgg
     2281 atagaatgtt gtcatggaat aaaattacct taaaaattga ataaagtatt actcttattg
     2341 actgatatga agaccatttt agaaaaatag attgcagtag aaatgcctat ttattaaatt
     2401 tacagggaat tctaatcttt aggagtcagc aatgaaaaca attactccga tcctgatcat
     2461 tctatttcat ctttcggcca cagattgggc gtgttcccaa tggacctgcc agaatccgct
     2521 gccacaggta aatacgctta acgacgtatt tttcatcgat ccggaaacag gctgggttgt
     2581 cggcgaagcc ggcaccatca tgaaatccac ggatggcggg caaacttatg acctcttaaa
     2641 tttccagact catctgaatt tttcctgcgt aaaattcttt gatctgaata acggccttat
     2701 agcaggcgat tccggcataa ttctcgtcag tgatgaagga ggttcgaact ggaacagagt
     2761 ggaaacaggc ttttctgcag atatcacgga tatatacatc attaataact gcatctcctg
     2821 gctgtcttgc actaacggta cgatcatgaa atcagatgat tttggcggat cgtggtctat
     2881 tgcatacgat aaccccttat tggagttcaa cagtctttca tttgcagatg aaatgcacgg
     2941 atgggcagct ggcaaagata atacaggttt tccagcgatt gttcgcacct gcgacggcgg
     3001 cctttcatgg atagagcttg atcccccttt tgaagatcaa atattcgata tctgtttcgc
     3061 tgacacattg gttggttacg cctctttaag ggatatagat tatgaaacca gacttgcaaa
     3121 aactacggat ggtggactat catggctatt tctgctggat gatgttttct atggagaatt
     3181 ttatgatatc gaattcattg acgccgagca cggctggatt gcaggttcca gtgcgtcaat
     3241 cccatctgca tggggtattt tcttttacac ttcagatgga gggacaacat ggaatccggg
     3301 tgacatcact catgattata atcaccgggc ttttaattct gtgtatttta ttaatgaaga
     3361 tacaggatgg atggtgggaa gcctgggtat aattcttcat tcaaatgacg gaggaataca
     3421 ctggtcaaaa agtaacaccg gatttaccgg tgattgccat tttgaggatg ttttctttgt
     3481 tgatgatcag aagggatggg cagtgggagc cggattctat ccttatgaat cgggcatcct
     3541 ccataccacg gacgggggaa attcatggac acctcaacca gccctcgagc atatgctcaa
     3601 cagcgttttc tttataaacc cgcttgaagg ctggattgtc ggcggaagat cgcttcagga
     3661 gtatacaacg gcaatttatc ataccaactc aggaggagat caatgggata aacaattttc
     3721 cgagaacatc aatgatgggg atttttcgga tgtctttttt aaggatgcta tgcatggatg
     3781 ggcggttggt tccgggaaca attattatcc gccaatgaat aactcattat tcttttatac
     3841 tgaagatggt ggcgaaaact ggattgatca atccgctatg atttctcatg ctttatcttc
     3901 aatttgcttt acagatgatg aaacaggctt tatttcaggt cacaagatca ttttacgttc
     3961 gataaacgga ggaaaaagtt ggactgaggt ttggaacggc tctcactatc tcaggaatat
     4021 ttttttcacc gacattaacc atggctgggc catcggagac agtgctacac gttacccata
     4081 ccgggacgta atcatgcgca caaccgacgg aggaacgacc tgggaagaac agttccccgg
     4141 gttcggatcc atgaaaaaac aattgttttt tacagataat agccatggct ggattgcgag
     4201 ggacgagggt gatatattgt atacaattga tggaggtatc acctggcaac atatgtttgt
     4261 aaactacaat tggtctttcg ggggtatatt ctttacggat attgacaatg gctgggtagt
     4321 cggaggaaac aaaaccatct ttcacatcaa taacggtggg actgtcggga taacggaacc
     4381 atcagccgcc ggcggtcatt cgccggttag cgcttacccc aacccaacca cagggattgt
     4441 cgaatttcga ttgtcgaatt tcgattgtcg atggatctca ttgaagattt atgatttgca
     4501 ggggcaggaa gtggcgactg tgatggatgg caggtggtac ggtagtaagg gagtaaggtg
     4561 ggatgcatcg ggtctgccgg caggagtgta tttctatcag ctcagaacga agggtgaagg
     4621 gcagggagcg gcagggaaga tcgtgaagtt gtaggtggtc aggtggtcag gtaaaattga
     4681 aaattgaaaa atcaaatgca aaatgccgaa tgcgcaatgc ggaatgtttt tcgcctttgc
     4741 gaaactttgc gctacctttg cgaaactttg cgatacaaca aatatcttac aaacatgaaa
     4801 aaggccctct tgctgatcag tcttatcttt atctctgtta gaatcttctc ccaggtggaa
     4861 gaaattaata tatacgaccc cgatgccaat gcccgcgaag atatcgcaaa tgccgtcagg
     4921 caggccgatg ccggaaacaa gcatgtgctt cttcagatcg gcggcaactg gtgcccctgg
     4981 tgcgtcaggc tgcaccacct ggaatcatcc gatccccaga tcgactcgct gctcaaagcc
     5041 gattacatcc gggtgatggt gaatgtgccc aaagaaaaag acaaacggga ttatgaattg
     5101 ctcaagacgc ttggcaatcc gcagcgtttc gggtttcccg tgctggtttt tctcaaccgg
     5161 aagggtgaac gcattcatac gcaggatagc tggtacctgg agcaggacaa agcctatgac
     5221 cgggaaaagg ttatccacct cttcaagatg tggaacgtag tggcagccgg caactaaaac
     5281 ccgttcagct ccatcgtcac cgttgggatc agcagtaaga atccaaggat gaaaaagata
     5341 aggataatcg gccagaccca gcggaaccac ctgtcgtaag ggatcctggc cactccgagc
     5401 gctccgatca aaacaccgga tgttggcgtg atcatgttgg tgaagccgtc gccaaactgg
     5461 aaagccatga ccgtcgcctg cctcgacagg ttaataaggt cggaaaacgg cgccatgatc
     5521 ggcattgtaa gggccgcttt ggcagaaccg gaaggaatta caaggttgat gatcgtctgg
     5581 atcacgtaca tcgtccctac ggcttgaatc tggcccgcct ggtgaagcga tgaagccagc
     5641 cggtgcaata tcgtgtcgat tacctttccg tcttccagta ccacgatgat gccgcctgca
     5701 agccctacta caagtgcggc tgaaacaatg tccctgacgc catccaggaa aagcttcacc
     5761 tgggtgttgc cgccgtatcc catggctacc ccggaggcaa tccccatggc aaagaacaac
     5821 gctgcgattt ccatgatata ccacgaatag cccatcaccc ctattacaag gaaaacaatg
     5881 gtgaacaaca gcagggtcag gatgaaataa tggaccgttt tttgtaaaga aatcagcccg
     5941 agaaaagcaa aaagtaccgt tgcaaccggt atcgccggaa tgatcattgc cgccgaattg
     6001 ccgatcttta tttcagtcat cggataataa acagagaaag caatgagcac cgcaagcgtt
     6061 aatgcgaagg atacccaggc agatacaggc gtatggtgct ccatccgctc tgtggttccg
     6121 gcagctttgt cacgccagta aacatcgata tcgtgaaccg gcgatctttg cgggttcttt
     6181 ttgatcctgc gggcatacca gaggacaaaa ccaattccta tgatattaat tatgataaag
     6241 cagaaaaagc ggtattccag ccctgaaaac aatggtaacc cggcaatccc ctgtgctatc
     6301 ccgatagtga acggattcag catcgcagca gcaaaaccga tatgggccgc caggtaacac
     6361 atggctacgc ctactatcga atcatatccc atcgaaatag ccagtggcac caggatgatc
     6421 acaaatgcga tcgactcttc actcatgccg aaaattgcgc cgaataagct gaacataagc
     6481 atgatcagca caatcacgac attatcgacg ccgatcagct tgaagaacct gaatttctca
     6541 agactgcggg tgaaatgaat gaaagacaga atgcccatat cgagcgcttt agtagtgttc
     6601 atgatccaga acgcaccgcc gatcatcagt attaaaacaa taataccggc ctgcctcaca
     6661 aaacccttga acatcgtgcc aaaaacctgc caggtctgcg gtgcattatc ctgctcgata
     6721 aagaccagct ctttaacctc cttgccatca acggttttga cctgctcgac ataatccccg
     6781 cccggaaaga tccaggttaa aatggcagcc aggattatca ggctgaaaat gatcacatag
     6841 gtatgcggaa acctcatttt catagttcaa agttcaaagt tcaaagttca aaattcaaaa
     6901 ataaacttat taccaacatt atgacgcgag cgaagcgagc ttatgacgca tgcgcagcat
     6961 gccaatgtcg cgagcgaagc gagcttatga cctcagaatt tctcatcata tttcgtccag
     7021 aggtaatcgc ccagtttcca cgctttgtcg accacatagc ttccccacat gccggtatag
     7081 gaggtcagga aagccttagc cttcccggga tcttccttaa gcatttgcac cgcctgtaat
     7141 tcgaccgatt tttgccggtc gaacagctcc tgctggatcg ggttccagat ggcgtcgaca
     7201 tcgtggcgca tatcgcccca gcgctgagcc gtcagcgtgc ctaatcgatt gaaggcccac
     7261 caggccgact tgttgctgaa gccctcgatc ctcccgtcga ccttgtaggg ttccggcagg
     7321 tcggtcacgc cgcagtagac cgggatatag accgatgtgg ccacattgtc catagccagc
     7381 cacaccaccc ctcccaccgg gtcgggcaac cattcgcggc attggatgat cgtggcatac
     7441 atcgtgtacc agcgggcgat ggtccgttca ccaagccatc cccatccccc gttgatttta
     7501 aagagtttgt tggcgtcgta tggcatatgc gggttggcca gcggcgaaat aattgatttt
     7561 ccatcatcgc cggttacagt catatctttc gtcatatcga acggcgtccc ttcgtagtaa
     7621 tccttaaaaa tggaaacgag ctgcggcagt gtaaccaggc tgtcgggttt tacggagaaa
     7681 gggtagttct ctgcattcgg atcgagcttc agagacggcg ccaccaggtc gagcacacgc
     7741 cattcgcgcc gccgtgaggc cagcgtggtg cggctttcgg gagcgtaagc atagcagaac
     7801 tcgaaatcgt ccttcgtggt gcaccatccg ctatcgatgg ctacctggaa gatgttttcc
     7861 gaagccataa agtgttcctt atccttgagg ttgatcttcc ggatacggct ggcgttggcg
     7921 tttaccgaca catggtcgtc gggtacgcgc tgggcggccc acaccgatcc cacctggtct
     7981 tttcccgggc cgaggatctc caggtgccac acctcttttt tatcggcgat ggtcaggcat
     8041 tcgcccgcat cgttatagcc gtattttgcc gtcagctcgc cggccatggc gattgcttcg
     8101 cgggccgtcg tgcatcgctc caccatcagc tggcatagca tcgtgcaatc gatcagccct
     8161 ttgcccgatt gcatcgactc ccttcccccg aaggttgatt cgccgatggc gagctggtgt
     8221 tcattaatgc aagggtaagc cgagttgaca taggcaaagg tgtgcttcac ctgcgggatg
     8281 gtccctatct tcttgtaaac atacagcggc atggcgaacg tgtcgcacgg cacccgttgg
     8341 gtgatatcgc accgggaacc ctccggatga tcgagcgccg gctggatgaa gatcgacgag
     8401 cgggtccggt ggctgtcgtc ggtatgggat gttattactg agccgtcaat ggtagcccgt
     8461 ttgccacagg tgatcgacgt gcagccgtcg ggcacggtgc caacccagtc ggatttgtcg
     8521 acctgggcgg agagatcatt tggagatgtg ataattagaa cgattaatag gatatacagc
     8581 tttttcataa gtgatgtttt tgttacaaga ca
//
LOCUS       DAOPNH010000140         8552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9166_length_8552_cov_5.903378, whole genome shotgun sequence.
ACCESSION   DAOPNH010000140 DAOPNH010000000
VERSION     DAOPNH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_8552_cov_5.903378"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..815)
                     /locus_tag="PKZ74_08495"
     CDS             complement(156..815)
                     /locus_tag="PKZ74_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM92836.1"
                     /translation="MKLSRYLALHLLLIMPFVAFTQPVDTEREEYYKGLVQFSGAVVT
                     GDSLKPVPFTHIIDKNTGFGTISDYYGYFSFVANKGDTITFSSVGFKKSSFIIPDTIH
                     NNRYTMFKIMTADTIYLSETVIYPWPTKEQFKEAFLKLDIPDDDLEIARKNLDRYELY
                     VRAEAIPMDGSMNYRNYIDQTVSKLYYAGQTQPISLLNPFAWAQFFQAWQDGKFKRKD
                     R"
     gene            complement(976..1215)
                     /gene="rpmB"
                     /locus_tag="PKZ74_08500"
     CDS             complement(976..1215)
                     /gene="rpmB"
                     /locus_tag="PKZ74_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPM92837.1"
                     /translation="MARVCEITGKKPIKGHNISHSNIKTLRRFLPNLQTKRFYIPEED
                     KWITLKVSTEAIRTINKKGITACLKEAREKGFTDK"
     gene            1479..2831
                     /gene="gdhA"
                     /locus_tag="PKZ74_08505"
     CDS             1479..2831
                     /gene="gdhA"
                     /locus_tag="PKZ74_08505"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM92838.1"
                     /translation="MSNNYENLVNEFMAKIIAKNPGEKEFHQAVREVTESLIPFIEEN
                     PKYKTAKILERMAEPERVILFRVPWVDDKGEIQINKGYRIEMNSAIGPYKGGLRFHPT
                     VNLGILKFLAFEQVLKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELARHI
                     GADTDVPAGDIGVGGREIGFMFGQYKRLRNEFTGVLTGKGLEWGGSLIRPEATGYGQV
                     YFAEEMLKTRGDSLKGKICCVSGSGNVAQYATEKATMLGAKVVTLSDSNGFIHDPDGI
                     TPEKLQFAMYLKNVKRGRIQEYAQKYGCAYYEGKRPWMIKCDVALPSATQNEVDGDDA
                     KTLMANGCFVVSEGANMPSTPEAIEVFLNAKILYGPGKAANAGGVATSGLEMSQNSMR
                     LSWSREEVDNKLHNIMINIHANCVKWGKGDDGYIDYVKGANIGGFVKVADAMIAQGIV
                     "
     gene            complement(2894..3949)
                     /locus_tag="PKZ74_08510"
     CDS             complement(2894..3949)
                     /locus_tag="PKZ74_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPM92839.1"
                     /translation="MKKNRIILIITLILIIIAAILVFSTSTSTLNRRVSDFSVQDTAS
                     VTKIFMADKSNNELLLERQPDGSWIVDGKFQVQQAKISAFLKTLKDLTVRSPVPLAAR
                     DNVIKRMAVLAKKVEIYQVKPAISLFNRVNLFPREKLTKVYYVGDVTQDNQGTYMLME
                     GADDPFIVHITGFRGFVSSRYSTIRADWRDFTVFKTPINRIRSVRVEYPQKPEQSFEV
                     DVLDNQNVSLVSLADNRNVAYDTLRTLHFLTSFEDVRFESLLAHLIEKEFIDSVSAST
                     PKTIISLTDTDGRVNRIKIFNKKSFAPYYSEDGAMLEPVDLDRAYALVNEEEDFVLIQ
                     YFVFDKVTRTLDFLTGK"
     gene            complement(3975..5717)
                     /gene="gldG"
                     /locus_tag="PKZ74_08515"
     CDS             complement(3975..5717)
                     /gene="gldG"
                     /locus_tag="PKZ74_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPM92840.1"
                     /translation="MAKNKQNNLMRNSLIQFGLGLLIIILANIIGSYWFTRFDLTSEK
                     RYSLSPVTKNLLKEIDDIVYFQVYLDGEFPAGFKRLRNATKEMLDEFRAYNKNIQYEF
                     VNPSASDNEQERNDTYQLLVEKGLQPTDLKVNTKEGLEQQIIFPGAIVTYKSNELPVE
                     LLNSQIGVDPEDVLNNSVQALEFRLASAIRELTVIMKPSVAFVRGHGELDNREIYDIG
                     QALSKQYSVSVVSIGGQLNSLTKRDSVSSDKTRIINKFDAIIIAKPDSAFSEKDKFII
                     DQFIMRGGKVLWLIDPVFASMDSLQRATETLGIGLDLNLEDMLFRYGVRLNANLIMDL
                     NALSIPLRTGQVGNQPQIDFFPWYYFPVITPSENHPIVRNLNAIKTEFISSIDTIKVA
                     GVKKTILLRSSPYSRTVNAPVLITLAILEQEPDERAYTGPQQPVAVLLEGELRSIFEN
                     RIPPEISTSRDIGFIPKSEPTKMIVVADGDIIRNQLHFSQGYPLPLGYDQYTRQSFGN
                     KDFILNALDYLIDESGLISIRSREVKLRQLDMPRVTKNKIFWQAFNVILPVIFVILFG
                     IIRYYLRKRKYTGI"
     gene            complement(5720..6451)
                     /gene="gldF"
                     /locus_tag="PKZ74_08520"
     CDS             complement(5720..6451)
                     /gene="gldF"
                     /locus_tag="PKZ74_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPM92841.1"
                     /translation="MLTLLKKEISSFLGSLTGYIVITVFLLINGLFLWVFEGDFNILD
                     FGFANLDGLFMIGPFVFLFLIPAITMRLFAEEKRTGTIEMILTKPLTELEIVMAKFLS
                     GLVLVIFSLLPTLIYFFSVYQLGLPKGNLDIGGMWGSYIGLLFLGASFVAIGMFASTL
                     TDNQIVSFILAVFLCGFLYVGFDLIHSFDLFGKADLFIKSLGINEHYKSMSRGVLDTR
                     DMIYFLGVIALFILLSRFSVERRKW"
     gene            complement(6530..7309)
                     /locus_tag="PKZ74_08525"
     CDS             complement(6530..7309)
                     /locus_tag="PKZ74_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPM92842.1"
                     /translation="MMPIITLTSDWGQRDYFLAAMKGKLMTLLPEASIVDISHEISPF
                     NLKQASFVIRNSYPHFPKGTVHVLSVLTEMNNKTPHLAVQYDGQYFIAADNGIFSLIF
                     DKQPEKIIRLSVQASNGIKEKPARDRFIEAAVHLASGKPVEQLGEPLGEWREQLHFHP
                     VVSGDVIRGVVIYVNRYENVITNITKDLVEKTGRGRNFIIEFRGEIIKGIKHSYQEAP
                     IGEIVALFGSTGHLEIAINQGNASSLLGLDINDPVRVEFKD"
     gene            7446..8405
                     /locus_tag="PKZ74_08530"
     CDS             7446..8405
                     /locus_tag="PKZ74_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPM92843.1"
                     /translation="MSERVISIEAYSPLEVYGVNDRNLDLIKKQFPALRIVARGEVMK
                     VFGEEEELSQFERKFGLLLLSYDKFGRITENDILQVMQGNEDDTGGLIRGQQPDDVLV
                     HGRDGRIVRALTINQQRMVSSSDANDLVIAIGPAGTGKTYTAVALAVRALKNKEVKRI
                     ILTRPAVEAGENLGFLPGDLKDKLDPYLQPLYDALRDMLPTQKLLTYIEDGTIEVAPL
                     AFMRGRTLDHAFAILDEAQNTTSSQLKMFLTRMGKSSKFIVTGDITQIDLPRNQESGL
                     VHAVKILRDIPGMDIIYLDQRDIIRHRLVVKIIEAYAKERKNP"
     gene            8418..>8552
                     /locus_tag="PKZ74_08535"
     CDS             8418..>8552
                     /locus_tag="PKZ74_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM92844.1"
                     /translation="MLNNVVVRTDFNLPGQKSVYHGKVRDVYNINDELMVMVVTDRIS
                     A"
BASE COUNT         2173 a         1995 c         2191 g         2193 t
ORIGIN      
        1 actgccacgt tggccagctc gatcggttta ttgtcctggt cgcggacatt tccggatagg
       61 atggcatttt gctgggcaac caggggttgg aaacccatta ttgcgatgag cagaacgacg
      121 atattcctgg tgagttgcat cccggtcatt cccatttacc tgtctttccg tttgaatttc
      181 ccatcctgcc aggcctggaa gaactgggcc caggcaaaag gattcaacag gctgattggc
      241 tgggtctgcc cggcatagta cagtttgctg actgtctgat cgatatagtt acggtagttc
      301 atactcccgt ccatcgggat agcctccgct ctcacataga gctcatagcg gtcgaggttt
      361 ttccgggcta tttccaggtc atcatccgga atatcgagct tgagaaaggc ttctttgaac
      421 tgctcttttg tcggccaggg ataaatgacg gtttcagata aataaatcgt atcggccgtc
      481 atgatcttga acattgtata gcggttatta tggatagtgt cggggataat gaacgagctt
      541 ttcttgaacc cgacggagga aaaggtaatc gtatcgccct tattggccac aaaggagaaa
      601 tagccgtaat aatcgcttat ggttccgaag cctgtgtttt tatcgatgat atgcgtaaac
      661 ggaaccggtt tgaggctgtc gccggtgacc acggcgcctg aaaactggac cagccctttg
      721 taatactctt ccctttcggt gtcgacaggc tgtgtaaaag ccacaaaagg catgatcaac
      781 agcaggtgga gggcaaggta acggctcagt ttcatccggc aaagtaatta tcgataatgc
      841 aaagcagcaa atttaatgtt aaaatgaaac ggtcaggagg cggttttatt ttgtgacgcg
      901 cattgtcgca cggtatgaca ataaaaaaga ggctgccgga atgctctgac agcctcttca
      961 tacaaagaaa tatttttact tatctgtaaa gcctttttct cttgcttctt tcagacaggc
     1021 ggtgatacct tttttattaa tagtccggat agcttcggtg gacactttca gggtaatcca
     1081 tttatcttct tccgggatat aaaagcgttt ggtctgcagg ttgggcagga accggcgtaa
     1141 tgttttaata ttggaatgag agatattatg acctttaata ggtttttttc cggttatttc
     1201 gcatacacgt gccatgttac tgaatgtttt tcggttattt ttaaaaaacg gagtgcaaag
     1261 gacggaatat ttttttaaat aaacaaatgt tttttaattc atgatgagac tccatctcaa
     1321 ttttcgagat ggagtctcat tctctaagat caaacttaaa ccatttctaa attgcatttt
     1381 tgcaaaataa ttttgtgaat cttttcacaa caccaaaatt attttgacta atattgctcc
     1441 gcttttgaga attttaacaa ccaaatattc taaagattat gtcaaacaac tatgaaaacc
     1501 tggtgaatga attcatggca aagatcattg ccaaaaaccc gggtgaaaag gaattccatc
     1561 aagcagtaag ggaagtgacc gagtcgctta tcccgttcat tgaagaaaac cccaagtaca
     1621 agacggccaa gatcctggaa cggatggccg agcccgagcg ggtcatttta ttccgcgtac
     1681 cctgggttga cgacaaaggt gaaattcaga tcaacaaagg ttaccgcatc gagatgaaca
     1741 gcgctatcgg cccctacaaa ggcggcctcc gcttccatcc caccgttaac ctcggtattc
     1801 tcaagttcct tgctttcgaa caggtattga agaacagcct tacgacgctc cccatgggcg
     1861 gcggcaaggg cggttccgac ttcgatccca aaggcaaatc agacaatgaa gtgatgcgtt
     1921 tctgccagag cttcatgacc gagctggccc gtcatatcgg tgctgatacc gatgtcccgg
     1981 caggcgatat cggtgtggga ggccgtgaga tcggtttcat gttcggccag tacaagaggc
     2041 tgagaaatga gtttaccggc gttcttaccg gcaaaggact ggagtggggc ggcagcctta
     2101 tccgtccgga agctaccgga tacggccagg tttactttgc cgaggaaatg ctcaaaaccc
     2161 gcggcgattc attgaaaggc aagatttgct gtgtctccgg atcgggaaat gtggcacaat
     2221 atgctaccga aaaagcgacc atgcttggcg ccaaagtcgt taccctttct gattccaatg
     2281 gatttattca cgacccggac ggcattaccc cggagaaact gcagttcgcc atgtatctca
     2341 agaatgtgaa acgcggacgc atccaggaat atgcccagaa atacggctgt gcttattacg
     2401 aaggcaaacg cccgtggatg atcaagtgcg acgttgcctt accatcagct acccagaatg
     2461 aagtcgacgg cgatgatgcc aaaacattga tggccaacgg atgcttcgtg gtttcggaag
     2521 gtgcgaacat gccctccacc ccggaagcta tcgaagtgtt cctgaatgca aaaatcctct
     2581 acggccccgg caaagctgcc aatgcaggcg gtgtagctac ttcgggcctc gaaatgagcc
     2641 agaactccat gcgtttgtca tggtcgcgtg aagaagttga caacaaactt cacaacatca
     2701 tgatcaatat tcatgctaac tgcgtgaaat ggggcaaagg cgacgacggc tatatcgatt
     2761 atgtaaaggg tgcgaatatc ggcggattcg tcaaagtggc cgatgcaatg atcgcccagg
     2821 gaatcgtata atattggtaa ttatccgaac caacttttcg gggggcttgc cgtgtggcag
     2881 gtcccctttt ttattatttc ccggtcagaa agtcgagggt gcgcgtcact ttatcgaaca
     2941 caaagtactg aatgaggacg aaatcctcct cctcattgac cagggcatag gcccggtcaa
     3001 gatccaccgg ttccaacatc gccccatctt ccgaataata cggagcaaaa cttttcttat
     3061 tgaaaatctt gatacggttc acccggccat cggtatcggt cagcgaaata atggtttttg
     3121 gcgtgctggc cgatacggaa tcgataaatt ccttttcgat caggtgagcc agcaacgatt
     3181 caaacctgac atcttcaaat gaggtcagga aatgcagtgt ccggagggta tcgtaagcca
     3241 cattccggtt atctgccaga gataccaggc ttacattctg attgtcaagt acatcaacct
     3301 caaaggattg ttccggtttt tgcggatatt cgaccctgac cgacctaatc cggttgatag
     3361 gcgttttgaa tacggtaaaa tccctccaat cggccctgat cgtgctatac cttgaagaaa
     3421 cgaaaccccg gaatccggta atatgtacga taaacggatc atcagcgcct tccatcagca
     3481 tataggtccc ctggttgtcc tgtgttacat ccccgacata ataaactttt gtcagtttct
     3541 cacgggggaa caggttgacc cggttgaaaa ggctgatagc cggttttacc tggtatatct
     3601 ccactttttt agctagaacg gccatacgct tgataacatt gtcgcgggca gccagcggta
     3661 cgggtgatct gaccgtaaga tccttcaacg tcttcaggaa agcgctgatc ttggcctgct
     3721 ggacctgaaa ctttccatcg actatccagc ttccgtcagg ctgacgttcc agcagaagct
     3781 cattattgct tttatcggcc atgaatattt ttgtaaccga tgccgtgtcc tgtaccgaaa
     3841 agtcgcttac gcgccggttt aatgtcgatg tggatgtgga gaagacgaga atggctgcta
     3901 taattatcag gatcagggtg atgatcagga tgatccggtt ctttttcatg tattttcagg
     3961 ttatattttg atgattagat gcccgtatat tttcttttcc ggagataata acggatgatt
     4021 ccgaaaagaa taacgaaaat aacagggaga atgacattga aagcctgcca gaaaatttta
     4081 ttttttgtaa cacgcggcat atcaagctgc cgcagtttca cttcccggct ccggatggag
     4141 atgaggcccg attcgtcgat caggtaatcg agggcgttca ggataaagtc tttgtttcca
     4201 aacgactgac gtgtgtactg atcatagccc agcggcaaag ggtagccctg ggagaagtgc
     4261 aactggttgc ggatgatatc tccgtcggca acaacgatca ttttcgtcgg ctcacttttg
     4321 ggaatgaacc cgatgtcgcg gctggttgag atttcggggg gtatcctgtt ctcgaagata
     4381 gaccggagtt ccccttccag cagcacggcc accggctgtt gcggccccgt ataggccctt
     4441 tcatcgggct cctgttccaa aatagccagg gtgatcagta caggggcatt gacagtcctt
     4501 gaatatggcg aggagcggag caggatcgtt tttttcacac cggccacctt gatggtatcg
     4561 atgctgctga tgaattccgt tttgatggca ttgaggttcc tgacgatggg atggttctcc
     4621 gatggagtga taaccgggaa gtagtaccaa gggaagaagt cgatctgcgg ctggttacca
     4681 acctgccccg tgcggagcgg gatcgagagc gcgttgaggt ccatgatcag gttggcattc
     4741 agcctgacgc cgtagcggaa gagcatatct tcgagattca ggtcgagccc gatgcccagg
     4801 gtctcagtgg cccgctgaag gctgtccatc gaagcaaaaa cgggatcgat cagccacagc
     4861 actttcccgc cgcgcataat gaactggtcg atgatgaatt tgtctttttc agaaaatgcc
     4921 gaatcgggtt tggcgataat gatggcatcg aatttattga tgatccgggt tttatcgctg
     4981 ctgacgctgt cgcgtttggt caggctgttc aactgtccgc cgatggaaac gacgctgact
     5041 gaatattgct tcgacaaggc ctgtccgatg tcgtatattt cgcggttatc cagttctcca
     5101 tggccgcgga caaaggcaac ggagggtttc atgataacgg tgagttcgcg gatagcgctg
     5161 gcaagcctga attcaagtgc ctgaaccgaa ttgttgagca catcttccgg atcgacgccg
     5221 atctgcgaat tcagcagttc caccgggagt tcattggatt tgtaggttac aatggccccg
     5281 gggaaaatga tttgttgttc cagcccttct tttgtgttga ccttcagatc ggtaggttga
     5341 agccctttct caaccaggag ctggtaggtg tcgtttcgct cctgttcgtt atcactggca
     5401 gaggggttga caaattcgta ctgaatattt ttattgtaag ccctgaattc atccagcatc
     5461 tctttggtgg catttctcag ccttttaaat ccggccggaa attccccatc caggtaaacc
     5521 tggaaataga cgatatcatc gatctctttg agcaggtttt tagtgacggg ggacagggaa
     5581 tagcgttttt cggaggtcag gtcgaaacgg gtaaaccagt aagagccgat gatattggcc
     5641 aggatgatga tcagcaatcc cagcccgaac tgtatcaggc tgttccgcat caggttattc
     5701 tgtttgttct tcgccatgtt taccacttcc tcctttcgac agaaaaacgg gataatagga
     5761 taaacagggc gatcaccccc aggaaataga tcatatcacg ggtatcgagc acgccgcggc
     5821 tcatcgattt ataatgctca ttgataccca atgatttaat aaagagatcg gcttttccga
     5881 agaggtcgaa agagtgtatc aggtcgaagc cgacatatag aaatccgcaa agaaatacag
     5941 cgaggatgaa tgaaacgatc tggttatcgg tgagagtgga ggcgaacatc ccgattgcta
     6001 cgaaagaagc acccaggaaa aggaggccga tgtaggagcc ccacatgccc ccgatgtcga
     6061 ggtttccttt gggaagcccg agctggtaaa ccgagaaaaa ataaatgagg gtcggtaaaa
     6121 gggagaaaat gaccagcaca agcccggata agaacttcgc cataacgatc tccagttctg
     6181 tcaacggttt ggtcaggatc atctcgatgg ttccggtccg tttctcttcg gcgaacaatc
     6241 gcatggtaat ggcggggatc aggaagagga aaacgaacgg accaatcatg aaaaggccgt
     6301 caaggttggc gaagccgaaa tccaggatgt tgaaatcacc ctcgaatacc cacaggaaca
     6361 ggccgttgat cagcaggaat accgtgatca cgatgtatcc cgtcagcgaa ccgagaaatg
     6421 agctaatctc ctttttgagt aatgtcagca ttttgtgtta gttccaggtt ccgggttcca
     6481 ggttccgggt tccaatttcc aaattttcaa attttcaaat tttcaaattt caatctttaa
     6541 actccaccct gaccgggtcg ttgatgtcga ggcccaggag gctggaggca tttccctggt
     6601 tgatagctat ttccaggtgg ccggttgagc cgaacagggc gacgatctca ccgatcggcg
     6661 cttcctggta cgaatgcttt atccctttga tgatctctcc gcggaattca atgatgaaat
     6721 tccttccccg gcccgttttc tcaaccagat cttttgtaat gttggtaatg acattttcat
     6781 aacggttgac gtagatcacc acacctctta tgacatcacc cgagactacg ggatggaaat
     6841 gcagttgttc gcgccattcg ccgagcggct cacccagttg ttcgaccggt tttccggagg
     6901 ccaggtggac ggcggcttcg ataaatcggt cgcgggccgg tttttccttg atgccattag
     6961 aggcctgaac cgaaagccgg atgatctttt ccggttgctt gtcgaagatc agggaaaaga
     7021 taccgttgtc ggcggcgatg aaatactgtc cgtcgtattg cacggccagg tgaggggttt
     7081 tattgttcat ttcggtcagg accgacagca catgcacggt tcctttcgga aaatgggggt
     7141 agctgtttct gataacaaaa gaggcctgct taagattgaa cgggctgatt tcgtgtgata
     7201 tatcgacaat gctggcttcc ggcaacaagg tcatcagttt tcctttcatg gcagcaagga
     7261 agtaatccct ttgtccccag tcacttgtca gcgttatgat cggcatcatg aaacagcagt
     7321 taataatccg gccttgccag tgagttgatt gattgatatt tcggccaaaa ttacagaaaa
     7381 cttcacagat gaagcgcaat ttttaatact tttgaacttc tgctccactt agttgttatt
     7441 ccattatgag tgaacgggtt atctccattg aagcatacag tccgctagag gtttacggcg
     7501 tcaacgaccg gaatctggat cttatcaaga aacagtttcc cgcgcttcgg atcgttgccc
     7561 gcggcgaagt gatgaaggtt tttggcgaag aagaggaact gagccagttc gaacggaaat
     7621 tcggcctgct tttactcagt tacgataagt ttggacggat cacggagaac gatatcctgc
     7681 aggtgatgca gggcaatgag gacgacaccg gcggcctgat ccggggccag cagcccgatg
     7741 atgtgctggt gcacgggcgc gacgggcgga ttgtccgtgc cctgaccatc aaccagcagc
     7801 ggatggtatc aagcagcgac gccaacgacc tggtgatcgc cattggcccg gcgggaacgg
     7861 gcaaaaccta tactgccgtt gcgctggcag tcagggcact gaagaacaag gaggtgaaac
     7921 ggatcatcct gacccgtccc gcggtggagg ccggcgaaaa cctcggcttt ctgccgggcg
     7981 acctgaaaga caagctcgat ccttacctcc agccactgta cgacgctttg cgcgatatgc
     8041 tgccgaccca gaagctgctg acctatatcg aagacgggac gatcgaggtg gctccgctgg
     8101 ccttcatgcg cggccgaacg ctcgatcatg cgttcgctat cctcgacgaa gcgcagaata
     8161 ctacttcgag ccagctcaag atgtttctca cccgcatggg aaaatcatcc aaatttatcg
     8221 tcaccggcga cattacgcag atcgacctgc cccgcaacca ggagtcgggg ctggtgcacg
     8281 ccgtaaaaat cctgagagat attcccggga tggatatcat ttatctcgac cagcgcgata
     8341 ttatccggca caggctggtg gtgaagatca ttgaagctta tgccaaagaa agaaaaaacc
     8401 cttaaatcag tatacttatg ctaaacaatg tagttgtcag aaccgatttc aatctgcccg
     8461 gccagaaaag cgtttatcac ggtaaggtca gggatgttta taatattaat gatgagttga
     8521 tggtgatggt ggtcaccgac cggatctcgg cc
//
LOCUS       DAOPNH010000141         8452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9313_length_8452_cov_8.065738, whole genome shotgun sequence.
ACCESSION   DAOPNH010000141 DAOPNH010000000
VERSION     DAOPNH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9313_length_8452_cov_8.065738"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /gene="metH"
                     /locus_tag="PKZ74_08540"
     CDS             complement(<1..2156)
                     /gene="metH"
                     /locus_tag="PKZ74_08540"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPM92845.1"
                     /translation="MGTMIQRYRLTEEDYRGERFKDCGRDLKGNNDLLSITRPQIIRE
                     IHNAYLEAGADIIETNTFNANAISQADYGMESLAYELNLASASLARQCADAFTLKDPS
                     RPRFVAGALGPTNKTASLSPDVNDPGYRAVTFDELYAAYYRQAEGLLDGGADALLVET
                     IFDTLNAKAALYAIQDLLEKRGLKVPIMVSGTITDLSGRTLSGQTVEAFLNSVSHVDL
                     FSIGFNCALGAKELRPFVEEIALKAPFYTSVYPNAGLPNPLGEYDDGPEEMASQLKEF
                     TDNRFVNIIGGCCGTTPEHIRAFSRIIEHAEARKVSVQPGSLRLSGLEPLVSFPGSNF
                     INIGERTNVAGSRVFARLIREEKFEEALAIARQQVENGAQVIDVNMDDALLDAERSMV
                     RFLSLLSADPDIARVPVMIDSSKWPVIEAGLKCLQGKAIVNSISLKDGEGPFREKAGK
                     IKKYGAAVIVMAFDEEGQAVTFERKTEICRRAYRILTEEVKFAPSDIIFDPNILTIAT
                     GIEEHNNYAVDFIKAVRWIRENLPHARVSGGISNLSFSFRGQDALREAMHSVFLYHAI
                     KAGLDMGIVNAGNMPVYEDIPEDLLHLLEDVILNRRKDATERLIVYAEKMQAKEAKQE
                     TVEEWRAGTVEERLKHALVRGIVDHVTEDAEEALKKYPRALDIIEGPLMDGMNHVGDL
                     FGSGKMFLPQVVKSARVMKKAVAVLEPALEPHPSAAL"
     gene            complement(2695..>3815)
                     /locus_tag="PKZ74_08545"
     CDS             complement(2695..>3815)
                     /locus_tag="PKZ74_08545"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92846.1"
                     /translation="DTAGYVSSFIGTPHLGPGETNNGIGYMKISPDGTKIALAIFGSD
                     ICEVFDFDASTGVVSNVITSEPVFDELFGVEFSPDSRFLYLTITSVSLPTPAYTPPSY
                     LFQMDMNAGTSIFEPGSFDTIAFDTTGSYFGGIQLGPDGRIYVSRSPYGNSVISVIQN
                     PKRKGSACNFISNAIDLQGRHSRFGFPNFIQSWFDLPHFSANSTGNNFEYAFTLQDDS
                     NIDEILWDFGDPGSSQNTSADFQPLHVFSSEGTYEVSVTEYAGRTAYGPYTETIVIPS
                     TSGIDDAVDVSGFCKVYPNPGNGDLRIIFDENQKRVMISVKNSLGHHLLGPVSYNNLA
                     EDSEVFLDISELGNGIFFILFQNEEGDNFTIKYLLREE"
     assembly_gap    3816..3915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3916..4646)
                     /locus_tag="PKZ74_08550"
     CDS             complement(<3916..4646)
                     /locus_tag="PKZ74_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92847.1"
                     /translation="MRFAFFILHLPGFRIFIPAILAFIVFLTDDLAGQSICEKATQGV
                     LNDAGHQASRWYFGEYAGLDFRQGNPVADLSNDWINAITSPAIISDSAGGILFFTNGR
                     WVFNRLGDTMPNGTGLHGFPGYPMPALIVPKPGCDSIYYIFTSHRPKQNPEDPQTIYG
                     LEYNEVNMNLDSGLGDITIKNNVLLEPEVSSKLSGTKHSNGIDYWVVAHRFNSNEFCA
                     FRVTQDGVDTAGYVSSFIGTPHLGPG"
     gene            complement(4659..4735)
                     /locus_tag="PKZ74_08555"
     tRNA            complement(4659..4735)
                     /locus_tag="PKZ74_08555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4699..4701),aa:Ala,seq:cgc)
     gene            complement(4823..6100)
                     /locus_tag="PKZ74_08560"
     CDS             complement(4823..6100)
                     /locus_tag="PKZ74_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPM92848.1"
                     /translation="MNTLKKPKSLEILNDDWMAVVVGFIIIALTILIYEFVPSLPDLF
                     KPKDKWSGTDFLTGFLSKPNLLRIVYIFVFFAILAWLGLKFAGKKTTYFLGSFAVIAL
                     LAILAQVISTHTSIKAVSLETVFFSILIGLLISNTVKLPEWLKQSVQSEFYIKIGLVL
                     LGTSVLFTKIMSAGALGLVQALVVVVSVWYIAFWISRKLKIDPEMSTMLASAVSICGV
                     SAAIATCGAIKGDSKKLSYVISLVLIVAIPMMIFMPYLAKWMGLSQEVAGAWIGGTID
                     TTGAVVASGTIIGEIAQKYAVIIKSAQNVLLGLAAFAISIYWSYSQKSPSAEKPTARI
                     IWDRFPKFVIGFILASLLFSFALDAETVKQAGDTIKGYQNIWFSMAFVCIGLETRFRE
                     IFTAENRKPLYAFLIAQTINIIITLIIAYLLFR"
     gene            6178..7296
                     /locus_tag="PKZ74_08565"
     CDS             6178..7296
                     /locus_tag="PKZ74_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPM92849.1"
                     /translation="MTMFHFTRISMISIILSLLFVFCDNTDKPDGTGDPANLSITVLS
                     VDMETGDVTLQATADNTVRYLLYIDAETNPADSNTTGYFEYSFGPGEATYEITVRAYG
                     ESGRYIKGSKEIVIQLVPDGPVPLDSGYFSALSYAGYSMVWQDEFNGSAVEPANWTFE
                     VGGEWYNNELQYYRSENAWVSEGTLTIEARKENFGGHNYTSARMITQNKKSFQYGRVD
                     IRAVLPEGQGIWPALWMLGQNISSVGWPKCGEIDIMEMIGGQGRENTVHSTMHWDDGN
                     GHASYGLSYTTTGKNFHEKYHVFSIIWDEYTIKSYVDDQLYFTIDITPAALSEFHQKH
                     FFIFNVAVGGIWPGNPDNTTLFPQQMKVDYIRVFQKSK"
     gene            7313..>8452
                     /locus_tag="PKZ74_08570"
     CDS             7313..>8452
                     /locus_tag="PKZ74_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM92850.1"
                     /translation="MKLNLYKLAISFFLLCFPVLLMAQKHTVTGKVSDEGGPGGIPGA
                     TVLEKGTTIGAITDADGNYSIAVANSNDTLVFSYIGYKTMEIPIMGRTKIDVTLSADL
                     TELDEVVVIGYGTQKKKVVTGSISTVDAEKIEKIPVLRIEQAMQGQAAGVQVTQMSGQ
                     PGEAPTVRIRGTGTTMDSNPLYVVDGMVVGGIDYLNPGDIQSIDVLKDAASAAIYGAR
                     AANGVVLITTKTGTAGKMSLNFSTYYGIQNVGKKLEMLDADEYRMIWNEAARNSNRAE
                     PFDLAEVPEANTDWQEYLFTKNAPVSNYELSIAGGNEKTTYASSVSYFSQQGIIGGEK
                     SQFDRITARLNGQVKATNWFTFGSDLAYSSLTRRGISSNGSFNTAY"
BASE COUNT         2151 a         2153 c         2068 g         1980 t
ORIGIN      
        1 agggccgctg atggatgggg ctcaagcgcc ggctcaagca ccgctaccgc cttcttcatc
       61 acccgggcgc tctttactac ctgcggcagg aacatctttc cggatccgaa aaggtcgccg
      121 acatggttca tcccatccat cagcggccct tcgatgatat ccagcgcccg cgggtacttc
      181 ttcagggcct cctccgcatc ttccgtaaca tgatcaacaa tccccctgac cagcgcgtgt
      241 ttcagccgtt cttcgacggt tccggcgcgc cactcctcta ccgtttcctg ttttgcctct
      301 ttcgcctgca tcttctccgc atagacgatc agccgttcgg tagcgtcttt ccggcggttg
      361 aggatcacat cttccaggag atgcagcaga tcctccggaa tatcctcata aacaggcata
      421 ttgcccgcat tgacaatccc catgtcgaga cccgctttta tggcatggta cagaaacacc
      481 gaatgcatcg cctctcgcag cgcatcctgg ccacggaagg agaacgaaag attgctgata
      541 cccccgctga cccgggcatg cggcagattc tccctgatcc atctcaccgc tttgatgaaa
      601 tcgaccgcat agttgttgtg ctcctcgatc ccggtggcta tggtcaggat attcggatcg
      661 aaaatgatgt cggacggtgc aaatttcacc tcttccgtta aaatccggta ggctctccgg
      721 cagatctccg ttttcctctc aaaagttacc gcctgcccct cctcatcaaa agccatgacg
      781 atgactgcag cgccgtactt tttaattttc ccggctttct cccggaaagg tccttcgcca
      841 tccttcaggc tgatcgaatt gactatggct ttcccttgca ggcacttcag ccctgcttcg
      901 atcaccggcc atttcgacga atcgatcatc acgggcaccc tggcgatgtc gggatcggcg
      961 gagagcaggc tgagaaacct gaccatcgat ctttccgcat ccagcagggc atcgtccata
     1021 ttcacatcga tcacctgggc gccgttttcc acctgctggc gggcgatggc cagcgcctcc
     1081 tcgaacttct cctcccggat cagccgtgca aacacacgtg atccggcgac gttggtgcgt
     1141 tcgccgatat tgatgaaatt gctgccggga aaactgacca gcggctccag gccgctgagg
     1201 cgcagcgaac cgggctggac agagaccttc cgtgcttccg catgttcgat gatccgggaa
     1261 aacgcccgta tatgttccgg cgtggtgcca caacaaccgc cgataatatt cacgaaccgg
     1321 ttatcggtga attcttttaa ctgagatgcc atctcctccg ggccgtcgtc atactctccc
     1381 aacggattcg gaagaccggc attcgggtaa acgctggtgt aaaacggcgc tttgagggct
     1441 atctcctcca cgaaaggcct gagctccttt gcccccaatg cgcaattgaa accgatactg
     1501 aacaggtcga catgtgaaac agaattgaga aaggcttcaa cggtctgccc ggatagtgtc
     1561 cgcccgctga gatcagtgat cgtaccggag accataatgg ggactttaag cccccttttt
     1621 tcgagcaggt cctggatggc ataaagagcc gccttggcgt tcagtgtgtc gaagatcgtc
     1681 tcgacgagga gggcgtcggc gcccccgtcg agcaatccct cggcctgccg gtagtaagcg
     1741 gcatagagtt catcgaaagt gacggcgcgg tatcccggat cgttaacgtc gggagagagg
     1801 gaagcggtct tgtttgtcgg gccgagtgcg ccggctacga agcgcggccg ggacggatct
     1861 ttcagggtaa acgcgtcggc gcattgccgg gctaaactgg ctgaagccag gttaagctca
     1921 taggccagcg actccatccc gtagtcggcc tgggatatgg cattggcatt gaaagtattg
     1981 gtttcgatga tatcggctcc cgcttcgaga taggcattgt ggatctcccg tatgatctgc
     2041 ggccgggtga tggatagcag gtcgttattc cctttcaggt cacggccgca gtctttgaaa
     2101 cgttctccgc ggtaatcctc ctctgtcagc cggtaccgct ggatcatcgt ccccatcgcc
     2161 ccgtcgagca gcagtacacg cgtttgtatt atttgatcaa tggtattcaa tggaaaaaag
     2221 tcgttagtcg ttaatcgttg gtcgcttgtc ctgagtccgc cgcaggcggg cgaaggatca
     2281 gtcatttgtt tttggtcatt tattaaaaat aacaatcgga caatagaaca attgagcctt
     2341 tttgaattag tgtgtggttt tatggatcac gccttcggca taagcattgc tgaaggctca
     2401 tgccatgcac ataggcattg aaagggagta gtttgatggt gcggtcattt tttgccggtt
     2461 ttcagaatgc aaaggtaata tttatattga ttctaattta cggacacttt tccgcgctca
     2521 ggataattgt ttaattcgcg atgaggctgt ctccaaagtc tatttttaac cacaaaggcg
     2581 cacaaagtac atcacaaagg cgcacaaagt atcagattat caaataataa actttgtgtt
     2641 ccttcgtgaa atccttggtg ttcctttgtg gtttaaacta atgatatagc caattcattc
     2701 ctccctgagc agatatttta tggtaaaatt atcaccctct tcattctgaa ataaaataaa
     2761 gaagatgcca ttccccaatt cggaaatatc caaaaagact tcagagtctt cggctaaatt
     2821 attataagag acaggtccaa gcagatgatg acccagcgaa ttcttcacgc tgatcatcac
     2881 tcttttctgg ttttcatcaa agattatcct gagatctcca ttgcccggat tcggataaac
     2941 tttgcaaaaa ccggaaacat caacggcatc atctattccg gaagtgctgg gtatgacgat
     3001 cgtttcggta tacgggccat aagctgtccg gcctgcgtac tctgtcaccg acacttcata
     3061 ggtcccttcg gaagaaaaca catgtaacgg ttgaaaatcc gctgaggtat tctggctgct
     3121 tccgggatcg ccgaaatccc ataaaatctc atcgatattt gaatcatcct gcagggtaaa
     3181 agcatactcg aaattgttac cggttgaatt cgcggaaaag tggggaagat caaaccatga
     3241 ctggatgaaa ttcggaaagc cgaagcggct gtgcctgcct tgcagatcga tggcgttgga
     3301 aataaagtta caggcgcttc cttttctttt gggattttga ataacgctga taaccgagtt
     3361 tccgtaaggc gaccgggaaa catagatcct gccgtcggga cccagctgaa tgcccccgaa
     3421 ataactgcca gtggtatcga aagcgatggt atcaaatgat cccggttcga aaatgcttgt
     3481 tccggcattc atatccattt gaaaaaggta agaggggggt gtataagccg gcgtcggtaa
     3541 cgacactgag gtaatggtca gataaagaaa ccgggaatcg ggtgaaaact ccaccccgaa
     3601 taattcatca aaaacaggtt cggaagtgat gacattggag accacgccgg tcgacgcgtc
     3661 aaaatcaaac acttcgcaaa tatccgatcc gaaaatcgcc agcgcgattt ttgtaccatc
     3721 cgggctgatt ttcatatacc caatcccatt gttggtctct cccggcccca ggtgtggggt
     3781 tccgataaaa ctggaaacgt agccggcggt atcaannnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnncccgg ccccaggtgt ggggttccga taaaactgga aacgtagccg
     3961 gcggtatcaa cgccatcctg tgtcacccgg aaagcgcaaa actcatttga attgaacctg
     4021 tgcgccacca cccagtaatc gataccgttg ctatgctttg tcccggataa ttttgacgaa
     4081 acttcgggtt ccagcagcac attattttta atggtgatat caccaagtcc ggagtccagg
     4141 ttcatattga cttcattgta ttccaggcca taaatggttt gaggatcttc cggattttgt
     4201 ttgggccggt gagaagtgaa aatataataa atactgtcgc agcctggttt cgggacgatc
     4261 agcgccggca tagggtatcc cggaaaacca tgtaatcccg tcccgttggg catcgtatcc
     4321 ccaaggcggt taaataccca tctcccattc gtaaaaaaca ggatcccacc tgcactgtcg
     4381 ctgataattg ccgggcttgt tatagcattg atccagtcat tgctcagatc cgccaccggg
     4441 ttgccctgcc tgaaatccag cccggcatac tccccgaagt accaccgcga ggcctggtga
     4501 ccagcatcgt tcagaacgcc ttgcgtggcc ttctcgcaaa tggactgacc ggcaagatca
     4561 tccgtcagga agacaataaa cgccaggata gccggaataa aaatccggaa accgggtaaa
     4621 tgcaaaatga aaaatgcaaa acgcaaaatg gaacatgttg gtggagtata ccggagtcga
     4681 accggtgact tctgcattgc gaacgcagcg ctctggccat ctgagctaat accccgaaat
     4741 gctaattctt aatttttaat ttttaatgag taattcaaaa ttaagaattc aaaattaaga
     4801 attaagaatt aatcgcctga tattatctga acaataaata tgcaataatt aaggtgatga
     4861 tgatattgat ggtttgtgcg atcaggaagg cgtacagggg tttgcggttt tcagcggtga
     4921 agatttcgcg gaaccgggtt tccaacccta tgcacacaaa agccatactg aaccatatgt
     4981 tctggtaacc tttgatcgtg tcgccggcct gtttgaccgt ctcggcgtcg agcgcaaatg
     5041 agaacaacag ggatgccagg ataaaaccga tgacgaactt cggaaagcgg tcccagataa
     5101 ttcttgcggt cggtttttcg gccgacggcg atttttgcga atacgaccaa tagatcgaaa
     5161 tggcgaaggc ggcgaggcca agcagcacat tctgcgccga tttgatgata acggcatact
     5221 tctgggcgat ctccccgatg atggttcccg aagcgaccac ggccccggtc gtatcgatcg
     5281 tcccaccgat ccaggcgccg gccacctcct gcgaaagccc catccatttg gccaggtaag
     5341 gcatgaagat catcatcggg atagcgacga tcaataccag cgaaataacg taagagagct
     5401 ttttgctgtc gccttttatg gcgccgcagg tggcgatggc cgccgacacg ccgcagatcg
     5461 aaacggccga ggccagcatc gtcgacatct ccggatcgat cttcagcttg cgcgaaatcc
     5521 agaaagcgat gtaccagacc gaaacgacaa caaccagcgc ctgcaccagc cccagcgctc
     5581 ccgccgacat gatcttcgta aacagcaccg aagtccccag caagaccagc ccgatcttga
     5641 tgtagaattc gctttgcacc gattgtttga gccattcggg cagcttcacc gtattgctga
     5701 tcagcaggcc gatcagtatg gaaaagaaaa cggtctccag gctcaccgct ttgatcgacg
     5761 tatgggtcga gatcacctgc gccaggatgg ccagcaaagc tatgacggca aacgagccca
     5821 ggaaataggt ggtcttcttg ccggcaaact tcagccctag ccaggccagg atagcaaaaa
     5881 agacaaaaat gtagacgatc ctcaaaaggt ttggcttcga caggaacccg gtcagaaaat
     5941 cggtgccgct ccatttgtct tttggtttga aaagatcggg caacgagggg acaaattcat
     6001 agatcaggat ggtcagggca atgatgataa aacccacaac gacagccatc cagtcatcat
     6061 tgagtatttc cagtgatttt ggctttttaa gggtattcat acttaaaatg gtttatttat
     6121 gggaatgcaa tgataagtat tattttaatg tatttttgaa tcagataaca ctaagatatg
     6181 acaatgtttc actttacccg tatatcgatg atctccatca ttttatcctt gctttttgtg
     6241 ttttgcgaca atacagataa gccagacgga accggcgatc cggccaatct gagcattacg
     6301 gtactgtcgg tcgatatgga aaccggcgat gtcactttac aagccactgc tgataatacg
     6361 gtcagatacc tgctttatat cgatgctgaa acaaatccgg cagattccaa tactaccggc
     6421 tactttgaat actctttcgg acccggtgaa gcaacgtatg aaatcacggt ccgggcctac
     6481 ggcgaatcgg gaagatatat caaaggcagc aaagagattg tgatccagtt ggttccggat
     6541 ggcccggtcc cgctcgacag tggatacttc agcgctcttt cctatgccgg gtactccatg
     6601 gtatggcagg atgaattcaa tggttcggcg gttgaacctg ctaactggac gtttgaagtc
     6661 gggggcgaat ggtacaacaa cgaactccag tattaccgtt ccgagaatgc ctgggtatcg
     6721 gaaggtacac tgactattga agcccgaaaa gaaaatttcg ggggccataa ctatacctcc
     6781 gcccgaatga tcacgcagaa taaaaagtca ttccagtatg gccgtgtcga tatccgggcg
     6841 gtattgcctg aaggacaggg aatctggccg gcattatgga tgctggggca aaatatttcc
     6901 tctgtcggct ggccgaaatg cggtgaaata gacattatgg agatgatcgg cggccaggga
     6961 cgggaaaaca ccgtacacag caccatgcac tgggacgacg gaaatggcca tgccagctac
     7021 ggactgagct acaccaccac cggtaaaaac ttccacgaaa aataccatgt gttttcaatc
     7081 atctgggatg aatacactat caagtcctat gtcgatgatc agctctattt tacgatagat
     7141 attacccctg cggctctcag tgaattccac cagaaacatt tctttatttt caatgtcgct
     7201 gtcggtggca tctggccggg aaatcccgat aataccacgc ttttccccca gcaaatgaag
     7261 gttgattaca tccgtgtatt ccaaaaatcc aaataactta aattataaac agatgaagct
     7321 aaatctttac aaattagcga tttccttttt cctcctgtgt tttccggtcc tgttaatggc
     7381 ccagaaacat accgtaacgg gaaaagtaag cgatgaaggc ggcccgggcg ggattcccgg
     7441 cgcaaccgtc cttgaaaaag ggacaacgat tggcgcgatt accgatgccg acggaaacta
     7501 ctccattgct gtcgcaaaca gcaatgacac acttgttttt tcttatatcg gttacaagac
     7561 catggaaatt ccaatcatgg gccggactaa aatcgatgtc actttaagtg ccgatttaac
     7621 cgaactcgat gaggtggtgg tgatcggcta tggcacccag aaaaagaaag tggtgacggg
     7681 ttcgatctct acggttgatg ccgagaagat cgaaaagatc cctgttctgc gcatcgagca
     7741 ggcgatgcag gggcaggccg ccggtgtgca ggtaacccag atgtcggggc agccgggtga
     7801 agcgccaacg gtgcgtatcc gcggaaccgg caccacgatg gactccaacc cgctctacgt
     7861 agtcgacggg atggtcgtcg gcgggatcga ctacctgaat cccggcgata tccagtcgat
     7921 cgacgtgctg aaagacgccg cctcggcagc catctacggc gccagggccg ccaacggcgt
     7981 cgtcctgatc accacgaaaa cagggacagc cggcaaaatg agcctcaact tctcgactta
     8041 ctatggaatc cagaacgtcg ggaaaaaact cgaaatgctc gacgccgacg aataccggat
     8101 gatctggaac gaagcagccc gaaacagcaa ccgtgccgaa cctttcgact tagcagaagt
     8161 cccggaagcc aatacagact ggcaggaata cctgttcaca aaaaatgcac cggtcagtaa
     8221 ttatgaactc agtattgccg gcggaaatga aaaaacaacc tatgcttcat ccgtttccta
     8281 tttctcacag cagggtatca tcggcgggga aaaatcacaa ttcgaccgta ttacagcccg
     8341 gctcaacgga caggtcaaag ccaccaactg gtttactttc ggcagcgacc tggcctattc
     8401 cagcctcaca cgcaggggga tctccagtaa cgggtcgttc aatacagcct ac
//
LOCUS       DAOPNH010000142         8429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9346_length_8429_cov_7.901600, whole genome shotgun sequence.
ACCESSION   DAOPNH010000142 DAOPNH010000000
VERSION     DAOPNH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9346_length_8429_cov_7.901600"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKZ74_08575"
     CDS             complement(<1..364)
                     /locus_tag="PKZ74_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPM92851.1"
                     /translation="MQPGKVLTVFAIVSLAGFALAMISFRPALPDDKKGAFEYKDFEK
                     SAKCRTCHVQFYEQWRQSMMSQAYTHHWDEIEYFDLAVAHAAKVPELKDVVDGCNGCH
                     APLAFAAGVLPPARPSENT"
     gene            complement(492..914)
                     /locus_tag="PKZ74_08580"
     CDS             complement(492..914)
                     /locus_tag="PKZ74_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92852.1"
                     /translation="MKNLKILSIFLGSLQIFIAAGAIPAGALFLADTSGARMGVTPEI
                     LGNSPLDSFLLPGLFLVLVNGLATGAAAVLTFKRHRYSGIAGLMLGIILCFWIIIQVK
                     WITVSSFMQPMFFGIGVVETLLGWRIMKLTEQENLLPH"
     gene            979..2262
                     /locus_tag="PKZ74_08585"
     CDS             979..2262
                     /locus_tag="PKZ74_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM92853.1"
                     /translation="MAEKKGINRRSFPKSFWTVVTMEFFERGSYYGMMSVLSVYLVLS
                     RNEGGLGFSKESVGVIKSTIVPMLYFLPIISGAIGDRFGYRRVLFFAFASMSLGYLLT
                     GLSNTYATVFGSLLLVALGAGFFKPMIAGTIAKVTDESNSALGFGIFYWSINLGAFLF
                     PLILVPWLKAISWSYIFFMAAIGTGWLILLNLFVFREPAREPNSKPISKVFIDMLAIL
                     KDYRFFLMLFIYSGFWVMYFQVYDSVLWYLTEKIDMAPVNRIINSFLGLFVSNPGWKF
                     EAEHVTAMNAGAIILLQILVSSIVKNTKTLPTMIFGIALGSIGMGMLAIAPSPWIFLL
                     SMFVFTLGEMIAHPKFISYVGLIAPPDKKAVYQGYSFLYGVIGSGIGGILGAWLYVFF
                     VEKNDNPSMLWLTFAMIGVMTIAGLLFYNRKFVKK"
     gene            2402..3331
                     /locus_tag="PKZ74_08590"
     CDS             2402..3331
                     /locus_tag="PKZ74_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM92854.1"
                     /translation="METKQFGNTDMKITRIGFGAWAIGGGNWAFGWGPQDDAEAVGAI
                     KKAVEVGMNWIDTAAVYGLGHSEELVGKAIKGMQQKPYIFTKCGLVWDENRRTGQSLK
                     AKSIRKECENSLRRLDVDVIDLYQVHWPVEEDIEEAWEMMATLKEEGKVRWIGVSNFN
                     IEQMKRCLAIAPVSSLQPPYSLISRDYEKEILPWCLEQNIGVIVYSPMGSGLLTGTMT
                     RERIQAMPADDWRKKSPFFNEPMLSKNLALADKLRVIGESQGYSAGEVAIAWTLHNPA
                     VTAAIVGGRSAEQVEGICRAEEVGISEEEKMML"
     gene            3513..3869
                     /locus_tag="PKZ74_08595"
     CDS             3513..3869
                     /locus_tag="PKZ74_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM92855.1"
                     /translation="MRQEIENRLVDFAADVVKIRPGLDRNDLAENLIKQLSRSSSSAA
                     LNYSEAIGAESRKDFIHKVSLVLKEIRESYNNLRIIRKSGLYKGNQEDIDKLVDECSQ
                     LVAIFHKTVLTLKNRK"
     assembly_gap    4025..4124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4213..5937)
                     /locus_tag="PKZ74_08600"
     CDS             complement(4213..5937)
                     /locus_tag="PKZ74_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPM92856.1"
                     /translation="MNILKIILPLIFCFWYHGIYSQLTHPRAWAEWEETDGVIIHQPN
                     FYLQEQPTPVELLIAEEWDSLYADLIRGLFDEGVNIYYILDTNDRPEYHSDIIDTMYL
                     KYDIDIHHPGFHTVIGCKDNYTKLTKWTRDHGPMNVFKNETDSLYFYLFSDDAKGAGA
                     VMRDYLGIPDTVFYQGGAGNIGSDGGNYIVDGHAVGIVNMGNGAVLPALKSFFGLDTV
                     YALANYLEHADYYLKMVDEETLIISSHHPDDYIYGSEPYTYSQDSAILSGLVAFFQEQ
                     VVSQYGRPMKVFSVPNPPSVCDDSLQLWWYTHYASYTNSLIVNRSVFVPQFDTPGHDS
                     TALAIYRQAMPGYRIVPVFSSRGATAGGGVHCLTNSVAASDPVLIRHYCLNDTVDVAA
                     GYDITATIMAAAGISEAFVNWSSDPLGPFQSVLMEKDSGDAYSGLIPYQTGMTEVYYY
                     ITAVSEKGRSGIKPMVAPDHTFHFTIRGGAPGWISQAEQAGGIVRIYPNPARNLVRID
                     LSEARLDFSSAMDLRIYDLLGNRIMAGITGGEKEFSVSLEHLAAGQYFVVVQDLKGRF
                     VSEKLLIF"
     gene            6129..6647
                     /locus_tag="PKZ74_08605"
     CDS             6129..6647
                     /locus_tag="PKZ74_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92857.1"
                     /translation="MPKRKTFLTLFFLAFFLTLSGQESSSSSPVTETQRELENLFSRL
                     TQSATEGAHFPESSVSGIETRSGHFIVPWQGEDLVSDYIIRAESRDYMKKTYGYYLGE
                     LYKNFIKNLDGSLDFISSDHYGEAKYRMMYHVKIDRDEFCYVYEAATNSHFIASMEAV
                     PPRMACNVLKCF"
     gene            6826..7224
                     /locus_tag="PKZ74_08610"
     CDS             6826..7224
                     /locus_tag="PKZ74_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92858.1"
                     /translation="MKNLILLFTGIVLLTGCDEHDPPNPENEYDIYFAGYTYEWEMLG
                     PTFDFAHYWKNGNGKLLSGLGMANSIFIDNGTVYIAGFIGDILFESKAVIWINGTEEI
                     LSDEISGANAVHVSNGVVYVAGWFIRQAQV"
     gene            7197..7589
                     /locus_tag="PKZ74_08615"
     CDS             7197..7589
                     /locus_tag="PKZ74_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92859.1"
                     /translation="MVYSAGTGVIGDEYRAILWKNNTAELLYDKSSFATALYMAGSDL
                     WVTGYYYYLNGLTDALVWKNGVLQSEITGNRFIPVAVYVNEDDVFVAGNSEDGALLFV
                     NGISQGLNTAPYPYDTIYMIKSMCVIPR"
     gene            8072..>8429
                     /locus_tag="PKZ74_08620"
     CDS             8072..>8429
                     /locus_tag="PKZ74_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92860.1"
                     /translation="MSGKSTLLTTIVLAVLLTANSALGQSVVVSSYFNASDPRDEWSE
                     LLVIGDNLNINNYTFRDNNSSQSNWQPLITFSNTIWSNLRPGTVVIVWHRLRGTNGAL
                     HPKDMDVSDGYLEVWAN"
BASE COUNT         2227 a         1834 c         2063 g         2205 t
ORIGIN      
        1 tggtgttttc cgatggccgg gcagggggga gaacacccgc cgcaaacgcc agcggggcat
       61 ggcacccgtt gcacccgtcg accacatctt tcagctccgg tacctttgct gcatgggcta
      121 ctgcgaggtc gaagtattcg atctcatccc agtgatgggt atatgcctgc gacatcatcg
      181 actggcgcca ctgctcatag aattgcacat ggcaggtccg gcatttggcc gatttctcga
      241 aatctttgta ttcaaaagcg ccttttttat cgtcgggcag tgccgggcga aacgaaatca
      301 tggcaagagc aaatcctgcc agcgaaacga tggcaaacac ggtcaatact tttcctggtt
      361 gcataagtag caagtttggt tgatctaaag ttagaaacca attccgtgca aatgatagca
      421 ggacaaatat tatcaggaaa tttataatgg gtctggataa gaatagatcc cggatcggat
      481 aagcgtgatt atcagtgggg aaggagattc tcctgctcag tcaatttcat tattctccat
      541 cccaggaggg tttccaccac cccgatcccg aaaaacatcg gttgcataaa gctgctaacc
      601 gtgatccatt ttacctgtat gatgatccag aagcagagga taatgcccag catcaggccg
      661 gcgatgccgg aatagcggtg cctcttaaag gttaagaccg cggccgcacc tgtggccagt
      721 ccattgacca ggaccaggaa caatcccggc aacaaaaagg agtcaagcgg ggaattgccg
      781 agaatctcag gtgtgacacc cattcgggca ccggatgtgt cggccaggaa cagggcgcct
      841 gccggaatgg ctcccgctgc aataaagatt tgaagggagc ccaggaagat ggaaaggatt
      901 ttcaggtttt tcatgaagca aatataaaaa cccggctgat attcttttgc tattttcgca
      961 attcaaagca gaaatcttat ggctgaaaag aaaggtatta accggcggtc gttcccgaag
     1021 agtttctgga ctgttgttac gatggaattt ttcgagcggg gctcctacta cgggatgatg
     1081 tcggtgctga gcgtttacct ggtgctgagc aggaacgaag gagggcttgg cttttcgaaa
     1141 gagagcgtcg gggtcatcaa aagcacgatc gtgccgatgc tttactttct gccgatcatc
     1201 tcgggcgcta tcggcgaccg tttcggctac cgcagggtgc tgttcttcgc ctttgcctcc
     1261 atgagcctgg gttacctgct gacaggcctc agcaacactt acgccaccgt tttcgggagc
     1321 ctgctcctgg tggccctggg cgccggtttc ttcaaaccga tgatcgccgg gacaatcgcc
     1381 aaagtcaccg atgaatcgaa ctctgccctt ggatttggca ttttctactg gtcgatcaac
     1441 ctgggcgctt tccttttccc gctgatcctg gtaccgtggc tgaaagcgat ctcctggtcc
     1501 tatattttct tcatggcggc aatcggcacc ggctggctta tcctgctcaa tctctttgta
     1561 ttccgtgaac cggccaggga gccgaattcg aaacccatca gcaaggtctt tatcgatatg
     1621 ctggccatcc tgaaagatta ccggttcttt cttatgttat tcatttatag tggtttctgg
     1681 gtgatgtatt tccaggtgta cgattcggtc ctgtggtacc tgacggaaaa gatcgacatg
     1741 gcgccggtga accggatcat caactctttc ctgggattgt tcgtcagcaa tcccggatgg
     1801 aagttcgaag cagagcacgt tacagcgatg aatgccggcg ctatcatcct gttgcagatc
     1861 cttgtttcaa gcatcgtaaa gaacaccaaa acgcttccga ccatgatttt cggcattgcg
     1921 ctgggatcga tagggatggg aatgctggct attgcaccga gcccgtggat tttcctgctt
     1981 tccatgtttg tctttaccct gggggagatg atcgcccatc cgaaattcat ctcttatgtc
     2041 gggttgatcg ccccgcccga taaaaaagcc gtttaccagg gttattcatt tctttacggt
     2101 gtgatcggca gcgggatcgg gggaattctc ggagcatggc tttatgtgtt ttttgtggaa
     2161 aagaacgaca acccttccat gctctggctg acgtttgcaa tgataggggt tatgaccatc
     2221 gcgggattgc ttttttataa caggaagttt gttaagaaat gattgatatt atgattttgt
     2281 gatattgtga tattatgata ttgtgatatt gtgatattgt gatattgtga tattgtgatt
     2341 ttgtgaactt atgatctttt gatattgtga atcaataata aaccgtttaa tttttgatga
     2401 tatggagaca aagcaatttg gaaatacgga tatgaagatt acccggatcg ggtttggggc
     2461 gtgggctatt ggcggaggga actgggcatt tggatggggg ccgcaggatg atgctgaggc
     2521 ggtcggcgct attaaaaaag cggtcgaggt cgggatgaac tggatcgata cggctgcggt
     2581 ttatggcctg gggcattcgg aagagttggt cggcaaagcc atcaaaggaa tgcagcagaa
     2641 gccttatatc ttcaccaagt gcggactggt ctgggatgag aacaggagga cagggcaaag
     2701 cctgaaggcg aaatcgatcc ggaaagaatg tgagaacagc ctgcggcggc tggatgtgga
     2761 tgttattgac ctttaccagg tgcactggcc cgtggaagaa gatatagaag aggcctggga
     2821 gatgatggcc acattaaaag aggagggaaa agtcagatgg ataggtgttt caaactttaa
     2881 tattgaacag atgaagagat gcctggccat tgcacccgtt tcatcgcttc agccgcctta
     2941 ttcgctcatc agccgggatt atgagaagga gatcctgccc tggtgcctcg aacagaatat
     3001 cggcgtgatc gtttattccc caatgggttc gggactactt accgggacca tgacccggga
     3061 acgtatccag gctatgcctg ccgacgactg gcgaaaaaag agcccctttt tcaatgagcc
     3121 gatgctgtcg aagaaccttg cactggccga taaattgaga gtgatcgggg aaagccaagg
     3181 gtattcggca ggtgaggtgg cgatcgcctg gacgctgcat aatcctgcgg taacggctgc
     3241 tattgttggc ggacgaagcg cagaacaggt ggaaggaata tgccgggcgg aagaggttgg
     3301 gatttcggaa gaggagaaaa tgatgttatg attttgtgag cttgtgaact tttgagattg
     3361 tgaacttttg agattgtgat tttgtggtgt ctggttgtgt gaagatgagt tgaaatatag
     3421 ctttgtaaat tattgaaaat cttttgcgta tgattttatt tttaatggtc cgggtatttt
     3481 ttggtagaaa accggattaa aaaataaaaa tcatgagaca agagattgaa aaccgactgg
     3541 tggattttgc ggctgatgta gttaaaataa ggccaggact tgatcggaat gatcttgcgg
     3601 aaaaccttat caaacaactt tcccgctcgt cttcaagtgc ggctttgaac tatagtgaag
     3661 ccatcggtgc tgaatcgaga aaagatttca ttcacaaagt cagtttggtt ctcaaagaaa
     3721 tccgggagtc atataataat cttagaatta tccgcaaatc gggactctat aaaggaaatc
     3781 aggaggacat tgataaactt gttgacgaat gcagccagct cgtggccata ttccacaaaa
     3841 cagtcttaac tcttaaaaac agaaaataat aatctgacca tcaattaatc acaacctcaa
     3901 aagttcgtaa gttcaaaata tcaaaagttc acaatttcaa aagttcacaa tctcaaaagt
     3961 tcacaatctc aaaagttcac aatttcaaaa gttcacaatc tcaaaagttc ataatctcaa
     4021 aagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcacaa tctcaaaagt
     4141 tcacaatctc aaaagttcac aatctcaaaa gttcacaatc tcaaaagttc acaatatcaa
     4201 aaaatcaaaa aatcaaaaaa tcaaaagttt ttctgaaacg aatcgtcctt tcaaatcctg
     4261 aaccacaaca aaatattgtc cggcagctaa atgttccagg gaaacggaaa attctttctc
     4321 ccctccggtt atccctgcca ttatcctatt tcccaaaaga tcatatattc ttaaatccat
     4381 cgctgacgag aaatccagcc gggcttccga taagtcgatc ctgacaaggt ttctggcagg
     4441 attggggtag atcctgacaa tgccgcctgc ctgctccgcc tggctgatcc atcccggtgc
     4501 tccgcccctg atggtgaaat gaaaagtgtg atccggggcg accatcggtt tgatgccgct
     4561 cctccctttc tccgatacgg ctgtgatgta ataataaacc tccgtcatgc cggtttggta
     4621 tggaataaga cctgaatacg catcgccgga atccttttcc atcagaacgc tttggaaagg
     4681 gccaagcgga tctgatgacc agttgacgaa ggcttcgctg atcccggcag cagccatgat
     4741 cgtggcagtg atgtcataac cggccgctac gtcgaccgta tcattcaggc agtaatggcg
     4801 gataaggacc ggatcggagg cggccaccga gttggtcagg cagtggacgc cgccaccggc
     4861 cgtagcaccc cggcttgaga aaaccggaac gatcctgtag ccgggcatag cctggcgata
     4921 aatggccagc gccgtgctgt cgtgcccggg ggtatcgaat tgcggcacaa acaccgaacg
     4981 gttgacgatc agcgagttgg tgtaggatgc gtaatgcgtg taccaccaga gctgcaggct
     5041 gtcgtcgcaa acggaaggcg gattcggaac agagaacacc ttcatcgggc gcccgtactg
     5101 cgagaccacc tgctcctgga aaaaggccac cagtccggaa agaatggccg aatcctggct
     5161 gtaggtgtag ggctcgctgc catagatata gtcatccgga tgatgcgatg agatgatcag
     5221 cgtttcttca tcaaccattt taaggtaata atcggcatgt tcgaggtaat tggccagggc
     5281 atagaccgta tccaggccga aaaagctttt cagcgccggc aaaacggcgc cattccccat
     5341 attcacaatg cccacagcgt gtccgtcgac gatataatta cctccgtcgg aaccgatatt
     5401 tcctgccccg ccctggtaaa agaccgtatc gggaatgccg aggtaatcgc gcataacggc
     5461 gccggcccct ttggcgtcat cgctgaaaag gtagaaataa agcgaatcgg tttcattttt
     5521 aaagacattc atcgggccat ggtcgcgcgt ccatttcgta agtttggtat aattgtcttt
     5581 acacccgatc accgtatgaa aaccgggatg atggatatcg atatcatatt tcagatacat
     5641 cgtatcgatg atatccgaat ggtattccgg ccggtcgttg gtatcgagga tataatagat
     5701 attcacccct tcgtcaaaga ggccacggat cagatcggca tacaggctgt cccactcttc
     5761 ggcgatcagc agttcaaccg gggtaggttg ttcctgcagg tagaaattcg gctgatggat
     5821 gataacgccg tcggtctctt cccattcggc ccaggcacgg ggatgggtca actgggaata
     5881 aataccgtga taccagaagc agaagatcaa aggcaaaatg atttttaaaa tattcatgat
     5941 gaatagataa acaagatatt ttccgaaaaa tattcccata attcaataaa ataagcattg
     6001 aattaacgga tgtgcagttt gacttggcag atgacacaag ataccaaagg tcataatctc
     6061 aaaagttcac aagttcagaa tatcacaagt tctcacaaaa tccataaatt agcaataaat
     6121 ttcgccccat gccaaaaaga aaaacttttc tgactctatt tttcctggcg ttttttctca
     6181 ccctttccgg ccaggaaagc tcgtcgtcat cacctgtaac tgaaacgcag cgggaacttg
     6241 aaaacttatt ttcccgatta acccaatcag caacagaagg cgcccatttt ccggagagtt
     6301 ctgtcagtgg gatagaaaca cgatccggcc attttatagt cccgtggcag ggagaagatt
     6361 tggtttcgga ctatattatc cgcgcagaga gcagggatta tatgaaaaag acctatggct
     6421 attacctggg cgagctttac aaaaacttta taaaaaacct ggacggcagc ctggatttta
     6481 tcagttccga tcattacgga gaggccaagt accggatgat gtatcatgta aagattgaca
     6541 gggatgagtt ctgttatgtt tatgaagcgg ccaccaattc tcattttatt gccagtatgg
     6601 aagcagttcc acccaggatg gcatgcaatg ttttgaaatg cttctgaacc atttccggga
     6661 ctatctggag ggagaaggat tttccgtgtc tgttgaaagt gaccattcga tgcgcggctg
     6721 gatacggact ctttaccttg ccaggcgcaa tgatatgact ttcaaattct tatatgagtc
     6781 ggagagtagt gacagcatgg gttactcgaa aatattgatt ataatatgaa gaatcttatc
     6841 cttcttttca ctgggattgt tctgcttacc ggatgtgatg aacatgaccc cccgaaccct
     6901 gaaaatgagt atgatattta ttttgccggt tatacatacg aatgggaaat gcttgggccg
     6961 acatttgatt ttgcgcacta ttggaaaaac ggtaatggaa agctattatc cggtcttggc
     7021 atggccaatt caattttcat cgataatgga acagtctata tagcaggatt tattggtgat
     7081 atcctgtttg aaagcaaggc cgtcatctgg attaatggca ccgaggagat cctttccgat
     7141 gagatttcgg gcgcaaatgc tgttcatgtt tcgaatggag tggtatatgt tgcggggtgg
     7201 tttattcggc aggcacaggt gtgatcggtg atgaatacag ggcaatttta tggaagaata
     7261 atacggcgga attattgtat gacaagtcat catttgctac tgcgctctac atggcaggaa
     7321 gcgatctttg ggtgaccgga tattattatt acctgaacgg cttaaccgat gctttggtat
     7381 ggaagaatgg cgttttgcaa agcgaaatta ccggaaatag atttatacct gtggctgttt
     7441 atgtgaacga agatgatgtt ttcgtagccg gaaacagtga agatggtgcc cttttgtttg
     7501 tcaacgggat atcacagggc ctgaatactg caccttatcc ctacgataca atctatatga
     7561 tcaaatcgat gtgtgtgata ccgagatgac agcaaagagg ctctgctttg gataactggt
     7621 caccgtttgc aaacagattc ctccttcgtc tttgctccgc tgaagctacg cgaaagcgat
     7681 gcgggatgac gcaggctgag gagggaccca ccggctctaa cgtcatcccg ggtttatata
     7741 ctattacaca ctccacaata tggaatgaca ttagcttttc attactccga agcatttccg
     7801 gtttccctcc ccaacccctc cctgtgaagg tttttctcta taatctaata tttccaaagg
     7861 gaggggcttt tttctttccc gtcctttttg ctacaaatag ggttctcctc cggagaacgg
     7921 ttcagtagaa attgctaact tgtcattggt agcgtagcaa gggacccaaa tcaatttatc
     7981 aacaaacccc attttaacaa cgtttactta acctttcctt ccctaaaata tagtatcttc
     8041 gccccctcat ttattataaa tcttaaccgc tttgtcaggc aaatctaccc ttctgacaac
     8101 catcgtctta gccgtccttc tcacggctaa ttctgccttg ggacaaagcg tcgtggtgag
     8161 tagctatttt aatgcatcgg atccccggga tgaatggtct gaattactgg ttattggcga
     8221 caacctgaat atcaataatt acactttccg ggacaacaat tcgagccagt ctaactggca
     8281 acctttgata acattttcaa acacaatatg gtccaattta aggcctggaa ctgttgtgat
     8341 cgtttggcac aggctccggg ggaccaatgg tgcactacac ccaaaagata tggatgtcag
     8401 cgatggttat cttgaagtat gggctaatg
//
LOCUS       DAOPNH010000143         8367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9449_length_8367_cov_4.824832, whole genome shotgun sequence.
ACCESSION   DAOPNH010000143 DAOPNH010000000
VERSION     DAOPNH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9449_length_8367_cov_4.824832"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1300)
                     /locus_tag="PKZ74_08625"
     CDS             complement(119..1300)
                     /locus_tag="PKZ74_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM92861.1"
                     /translation="MFLRPYYSRIEQRLGMERKFIQVLMGPRQVGKTTLVRQFLDKYP
                     FPWHYASADEATQPLWLQQQWETARLKLNSSGAKEALLVIDEIQKIPDWSNLVKIQWD
                     KDSFDKLPLKVILLGSSQLMLQKGLSESLAGRFEVTLLPHWSYSEMKEAFGFTLDDYI
                     WFGGYPGPADLISDESRWKEYIRHSLVETTIMRDILLLTRIDKPALLRNLFDLACSWS
                     GQILSYNKILGQLKDAGNTTTLAHYLKLLEGAGMVKGLEKYSSSTIRQRSSSPKFQVF
                     NTALMSALSPNQKQEIEQQHEIYGRWVESAIGAHLVNQSQAEQIHLFYWREGNHEVDF
                     VMQKGSKVIGIEVKSGRNKSSSGISEFTKRFNPDKVLLVGREGIPVKEFLTIPVPDLF
                     S"
     gene            complement(1508..2935)
                     /gene="mnmE"
                     /locus_tag="PKZ74_08630"
     CDS             complement(1508..2935)
                     /gene="mnmE"
                     /locus_tag="PKZ74_08630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPM92862.1"
                     /translation="MIHPAREDTICAIATPPGTGAIAIIRMSGNESLRICEQVFTPRN
                     RRLKVSESPSHMVHFGNIVWGEEMIDEVLVSVFRQPHSYTGENAVEISCHGSVYIQQK
                     IMELLISNGARTATPGEFTLRAFLNGKFDLSQAEAVADLIAASSRTAHDLALDQMRGG
                     FSKKIGELRTRLLDFASLIELELDFSQEDVVFADRKELVKLLEEIKSEIMHLASSFSY
                     GNVLKTGIPVAITGKPNVGKSTLLNAILNEERAIVSDIPGTTRDTIEDTIVVQNYAFR
                     FIDTAGLRHSRDAIESEGIERTYEKIRQAKIVLYVFDVRDADGARFLNQFRVPGSGFQ
                     DVEEDLRLQTPDDKKFILIGNKSDLLEETPAHLKELLEEHCIFISAKRKENINLILER
                     LVEEVREMHVQDTAVVSNVRHYEALLGAQKSAEAVLAGLSAGLPGDLISVDLRMALYH
                     LGEITGQITTDDILGNIFGRFCIGK"
     gene            3083..3955
                     /gene="sucD"
                     /locus_tag="PKZ74_08635"
     CDS             3083..3955
                     /gene="sucD"
                     /locus_tag="PKZ74_08635"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPM92863.1"
                     /translation="MSVLVNKHSRVLVQGFTGKEGTFHASQMIEYGTNVIGGVTPGKG
                     GTFHLDKPVFDTVSQAVDREGADVSVIFVPPAFAADAIMEAADAGIKVIVCITEGIPV
                     KDMIVAKDYLQCRNTRLVGPNCPGVITPGEAKVGIMPGFIHTPGPIGIVSRSGTLTYE
                     AVDQLTKTGLGQTTAIGIGGDPIIGTTTLDAVRLFTKDPDTKGIVMIGEIGGPMEAEA
                     ALWLKENGTKPVVAFIAGATAPKGRTMGHAGAIIGGKEDTAQAKKAILRECGIHVVDS
                     PADIGKRMKEILGN"
     gene            complement(3952..4803)
                     /locus_tag="PKZ74_08640"
     CDS             complement(3952..4803)
                     /locus_tag="PKZ74_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM92864.1"
                     /translation="MKRAVVILNILLIINGFNLFAQLPDFCLTRDEYRLYSLINAYRT
                     KNGQNVIPVSRSLCYVAKIHARDLYINNPDTGNCSLNSWSDQGNWTACCHSARTPNPV
                     CIVNKPKELARYAGEGHELAYWDSQDLHADTVLKFWQSYEQSKELLLNQKKWSHFNWK
                     AIGVGMYKGYACVWVGEARDTLPEPGLCPDDVDALTLPSGKIYKEVITSHTGRYYIIF
                     GSFMTQEDAMKTADKYRKEGLKEAKVLISGKTYRVSLADYPSQQDAVNAKKMLGERFK
                     EAWITKY"
     gene            complement(5106..>8367)
                     /locus_tag="PKZ74_08645"
     CDS             complement(5106..>8367)
                     /locus_tag="PKZ74_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPM92865.1"
                     /translation="RLDGDATGDSRQVVITISPAARVLTTLTIDDVPAPLTVTGVNNG
                     QFAGTGLEPSPATGRLDSDAWATTGMDEGISTYGDTKISGDFARGVSNGNVSLGTGGF
                     YSFQVATGDYAFGTQPTGNDWTPGTMTLRIKNNTGLSISGISFSYNICVYNDQPRSNS
                     FNFSYSIDSSAYIQITDLNYSTPTTATSGWTIVPRSYLISGLSIANGASFFFRWNSDD
                     VLGTGGRDEIALDDITINVTAEGVSNPAGFNAESVSPYQIDLTWNLNNNNDSVLIATN
                     LSNTFGTPAGDYSLGSEISGGGTVIYKGTGIAFSHSGLTSNTAYYYKAWSMTVANEYS
                     TGTEDNATTLKIQPSEYPTLFAVADTGITITATWTDAGGTQPPDGYLIRISSQDNIAA
                     PVDGTPVTNDLDLSDGTGAVSVAQGVGKYTFYRLQGTTTYYLKIFPYTNSGSGIDFLT
                     DDPAPAGNATTQSIVNTNDFESGLFGDWDTVSLAVKSRNWSIYATNGAYGTAKSGGIN
                     GYQTTDTLENDWLISPSLNLDAFADEYMVFFTWWRYGTDPNELKLKYSTDYVGGDPSL
                     ATWAELSFAKSSVEQTWTPSGFIDLKGISGTNVHLAFHYLSVTGPRWWLVDEIEITGD
                     GISNPADFSAQTASMTEISLSWTKNTSLNDVLVAFNTIDEFGIPEGQLDAGAEIPGGG
                     TVLYHGQDQSFLHTELTPATTYFYKAWSVDPDNNYSPGVAGSATTQYPEPSGNPTGLT
                     ATANGFSNITVTWTDSDASGYLVKASSTGFNAIAAPVDLVPQSDSLLVKNVAASVQSV
                     QFNGLIPATTYYFKIYPYNGSGSTINYKIDGEIPEASATTGELDPEIFISEVAESADN
                     NNIRFVEIHNAGTTAFDFNTIPVFLCKQANGGATWNSIQLSGTLAPGGNHIVAYSASY
                     FDTAYSSAANQYSTTVCNYNGNDGIFLYYRGNQLTGLLFDAFGRINQEGHDTTGWNYE
                     DGHVVRKREVSSSNPVWTAAEWSIIKTTNYDQMTPYLHKADVTWQGTTSTDWNRRGNN
                     WSGAQGWVPDASCNVIIPNVTNYPVVTRKSACNLLDIQTGSALSIQSTGTLQIVGP"
BASE COUNT         2024 a         2070 c         2069 g         2204 t
ORIGIN      
        1 gtatggttat tttagtcgct tccatttttt tcttattgca attaatttga cttcaccccc
       61 tctccgggtg gagagggggc agggggtgag gcctgaacaa tgcggcaact gtgtttaatt
      121 atgagaacaa atccggtact ggaattgtca gaaattcttt caccggaatc ccttctcttc
      181 caacaagaag aaccttgtcc ggattaaatc gcttggtaaa ctcagaaata cctgaagagg
      241 acttattacg accgcttttc acctcaatac caatgacttt gcttcctttc tgcataacaa
      301 aatccacttc atggttgcct tccctccagt aaaacaggtg gatttgttct gcctgggatt
      361 gattgaccag gtgagcgccg atggcggatt caacccagcg gccgtagatc tcatgctgtt
      421 gttcaatttc ctgcttttga ttcggactta acgccgacat cagggcagta ttgaaaacct
      481 ggaacttcgg acttgaagac cgctgtctga tggttgatga agaatacttc tccagtcctt
      541 taaccatgcc ggctccttca agaagtttca ggtaatgtgc cagggtagtg gtgttgccgg
      601 catccttcaa ttgtcccagt atcttattat aagataggat ctgaccagac catgaacagg
      661 caagatcaaa caggttcctt agcaatgccg gtttatcaat cctcgtcagc agcaaaatat
      721 cacgcatgat cgtcgtttca acgagggaat gacggatgta ctctttccag cgggattcat
      781 cggagatcag atcagcaggg cccggatacc ctccaaacca gatgtaatca tccagggtga
      841 agccgaatgc ctctttcatc tctgagtaag accagtgagg aagcagcgtc acttcaaacc
      901 gtcccgccaa cgattcactc aggcctttct ggagcatgag ctgtgatgat cctaggagaa
      961 taacctttaa aggcagctta tcgaagctgt ctttatccca ttggatcttc accaggttgc
     1021 tccaatcggg aattttttga atttcatcga taaccagtaa cgcttcttta gctcctgatg
     1081 agtttagttt tagcctggct gtctcccatt gctgttgcag ccagagtggt tgagtagctt
     1141 catcggctga tgcataatgc catgggaaag gatatttatc taaaaattgc cggacaaggg
     1201 tggttttgcc cacctgccgg ggacccatca gaacctggat gaatttcctt tccatgccaa
     1261 gacgctgctc gatccgggag taatatggac gcaggaacat aaagaatttt tcacaaagat
     1321 aataaattac tcaacgtatt gagtaaaatt actcagtgta ttgagtaaat aaaaatttct
     1381 aatgttctgt ccaactgtcc aactgtcaac tgtccaactg tccaactgtc aactgtccaa
     1441 ctgtccaact gtccaactgt caactgtcaa ctgtccaact gtccaaccgt ccaattgtcc
     1501 aactgaatca ctttccaata cagaatcttc cgaaaatatt ccccaggatg tcgtcggtgg
     1561 tgatctggcc ggtgatctca cccaggtgat agagcgccat acggagatcg acggagatca
     1621 ggtcgccggg gaggccggcg gagaggccgg ccaggaccgc ctccgctgat ttttgggcac
     1681 ccaacagcgc ttcgtaatgc cttacattcg aaaccacagc ggtatcctgt acatgcatct
     1741 ccctgacctc ctccaccagc cgttcgagaa tgagattgat gttttccttc cttttcgcag
     1801 agataaatat gcagtgttct tccagtaatt ccttcaaatg cgccggcgtc tcctccagca
     1861 gatcggattt gtttccgatt aatatgaatt tcttgtcgtc aggggtctgc aaccggaggt
     1921 cttcctccac atcctggaac ccggaacccg gaacccggaa ctgattcagg aaccttgcgc
     1981 catcggcatc acgtacatcg aacacgtaca gcacgatctt tgcctgcctg atcttctcgt
     2041 atgtcctctc gatcccttcc gactcaatgg cgtcgcggct atgccggagg cccgccgtgt
     2101 cgatgaaccg gaaagcgtag ttctgaacca caatggtgtc ttcgatcgtg tcgcgcgtgg
     2161 tgcccgggat gtcgctcaca atcgctcttt cctcgttcag gatggcattc agcagtgtcg
     2221 atttgcccac gttgggtttg ccggtgatcg ccaccgggat accggttttc agcacattcc
     2281 cgtaggagaa actggatgca agatgcatga tttcggattt gatttcttcg agcagtttga
     2341 ccaactcctt ccggtcggca aacaccacat cttcctggct gaagtcgagc tccagctcga
     2401 tcagggaagc aaaatcgagc agtcgtgtcc gcaattcgcc gatttttttc gagaatccgc
     2461 cgcgcatctg gtccaatgcc aggtcatggg ctgttcgcga agacgctgcg atcaggtcgg
     2521 cgaccgcttc cgcctgcgac aggtcgaact tcccgttcag aaaggccctc agcgtgaatt
     2581 caccgggggt ggcggtacgc gcaccattgc tgatcagcag ttccatgatc ttttgctgga
     2641 tgtagaccga accgtgacac gatatctcaa cggcattttc gccggtgtag gagtggggct
     2701 ggcggaaaac gctgaccagc acctcatcga tcatttcctc cccccaaacg atgtttccga
     2761 aatggaccat atgcgaaggg gattcggaaa cttttaatct gcggttcctg ggagtaaata
     2821 cctgttcgca aatcctgagc gattcgttgc ctgacatgcg gatgatggca atcgccccgg
     2881 tccccggagg ggtggcaatg gcacaaatag tatcttctct tgccggatgg atcattatta
     2941 tcagctttgt taacaaattt aaggatttct gccataaaaa tataatttgt tatatttgtc
     3001 gcatgccagc ccccttagga tggaaacata caaactccct aattaagcat taagaattaa
     3061 acattaagaa ttaaattctc ccatgagtgt tctcgtcaac aaacacagcc gcgttctggt
     3121 ccagggtttc accggtaaag aaggcacttt tcatgcaagc cagatgatcg aatacggaac
     3181 caatgtgatc ggcggggtta ccccgggcaa aggaggaacg ttccacctgg ataagccggt
     3241 gttcgatacc gtcagccagg cagtcgaccg ggaaggggcc gatgtgtcgg ttattttcgt
     3301 accgcctgct ttcgctgccg acgccatcat ggaggccgcc gatgccggga tcaaagtgat
     3361 cgtttgtatt acggaaggga taccggtgaa agatatgatc gtggccaaag actacctgca
     3421 gtgccgcaat acgcggttgg tcgggcccaa ttgtccgggc gtcattacgc cgggcgaggc
     3481 gaaggtgggc atcatgcctg ggtttatcca cacaccgggc ccaatcggga tcgtttcccg
     3541 ttccggaacc ctcacctacg aggcggtcga ccagctcacc aagacaggtc tgggccagac
     3601 aacggctatt ggcatcggcg gtgatccgat catcgggacg accacgctcg atgccgtcag
     3661 gctgttcacg aaagatccgg ataccaaagg gattgtgatg ataggggaga tcggcggacc
     3721 gatggaagcg gaagctgccc tgtggctcaa agaaaacggg accaagccgg ttgtggcttt
     3781 cattgccggg gctactgcgc cgaaagggcg tacgatgggc catgccggcg ccatcatcgg
     3841 tggaaaagaa gatacggccc aggccaagaa ggccattctg agagaatgcg ggatccacgt
     3901 ggtagattca ccggccgata tcggcaagag aatgaaagaa atccttggta attagtattt
     3961 tgtgatccag gcttctttaa atctttctcc cagcatcttc tttgcgttca ctgcatcctg
     4021 ctgcgaggga taatcggcca gcgacacccg gtaggtcttt ccgctgatga gaacctttgc
     4081 ctctttcagc ccttctttac ggtatttatc agccgttttc atggcatctt cctgtgtcat
     4141 aaagctgcca aagatgatgt aataccttcc ggtatggctg gtaatcacct ctttgtaaat
     4201 tttacctgac ggcaacgtca gggcatcaac atcatcaggg cagagaccgg gctcaggcag
     4261 tgtgtcgcgg gcttcaccca cccatacaca ggcatagcct ttatacatcc ctaccccaat
     4321 ggctttccag ttgaaatggc tccatttttt ctgattgagc aacaactcct tcgattgttc
     4381 atacgactgc cagaacttca gcaccgtgtc ggcatgcaga tcctggctgt cccagtaggc
     4441 cagttcgtgc ccttcacctg cataacgggc cagttccttc ggtttattga caatgcagac
     4501 cgggttggga gtcctggcgc tatggcagca ggctgtccag tttccctggt ccgaccagct
     4561 gttcaggctg caattaccgg tatccggatt gttgatgtaa aggtcgcggg cgtggatttt
     4621 tgccacgtag cagagcgaac gggagaccgg gatgacattc tgcccgtttt ttgtgcggta
     4681 ggcattgatc agcgagtaaa gccggtattc atcacgggtc aggcaaaaat cgggtaactg
     4741 ggcaaataag ttaaaaccgt tgattatcag taatatattc aggataacta cagcccgttt
     4801 cataagtgaa ggtattattc tacaaattta cagtttacgg tttacaattt acaatctaaa
     4861 atcttgagcc tatttaaaaa agcctttcat tgccactaaa gcaccaacac acgaaagcaa
     4921 acgaacaata acactttgat aatcaatttt atgtgctatt ttgtgtattt gtgattttgt
     4981 ggcatttcct gggaaattga ctattcatag tatactcatt cttgatctct gatactaaat
     5041 tcaatattca atattctaga ttctagattc tagattgtaa attgtaaacc gtaaactgta
     5101 aattcctatg gcccaacaat ctgcaacgtg cccgtgctct gtatgctcaa tgccgatccc
     5161 gtctggatat ccagtaggtt gcacgccgat ttccgggtta cgaccgggta attggtaacg
     5221 ttgggtatga tcacgttgca actggcatcc ggcacccagc cctgtgctcc gctccagtta
     5281 ttcccccgcc tgttccagtc ggtggaggtg gtgccctgcc aggtcacatc ggccttgtgc
     5341 aggtaagggg tcatctggtc gtaattggtt gtcttgatga tcgaccattc ggcagccgtc
     5401 cagaccggat tggaggagct aacctctctt tttctgacca catgcccatc ctcataattc
     5461 cacccggtgg tatcatgccc ttcctgattt atccttccga aagcatcaaa aagcaaaccg
     5521 gtcaactggt ttccgcggta ataaaggaaa atcccgtcgt tgccattgta attgcaaacc
     5581 gtcgtgctgt actgattggc ggcggagctg taagctgtgt caaaataaga agcgctataa
     5641 gctacgatat ggtttccacc gggagccaac gttcccgata attgaatgga attccacgtg
     5701 gcgcctccgt ttgcctgttt gcaaaggaag acaggtattg tattgaagtc gaaagcagta
     5761 gttcctgcat tgtggatttc cacaaaccgg atattattat tatctgccga ttctgcaact
     5821 tccgaaatga atatctcagg gtcaagttcg ccggttgtgg ccgatgcttc cgggatttcg
     5881 ccgtcaatct tgtaattaat tgttgacccg cttccgttgt aaggataaat tttaaagtaa
     5941 tacgtggtag ctggtattaa cccgttgaac tgaacggatt gtacagaagc tgcgacattt
     6001 tttaccagca ggctgtcact ttgcggcacg aggtcgacag gagctgcgat tgcattaaat
     6061 ccggtcgatg atgcctttac caggtagccg gatgcgtcgg aatcagtcca ggtgacggta
     6121 atattcgaga atccgttcgc cgtagctgtc aagccggtcg gattacccga gggttcaggg
     6181 tactgtgtgg tagcgctgcc tgccacgccg ggagaataat tattgtccgg atcaaccgac
     6241 caggctttgt aaaagtaggt ggtggccggg gtcagttcgg tatggaggaa tgattgatcc
     6301 tgaccgtgat aaagcaccgt tccgcctccg ggaatttcgg ccccggcatc gagttgcccc
     6361 tccggtattc cgaattcatc gatggtgttg aatgcgacta aaacatcgtt aaggcttgta
     6421 tttttagtcc aactcaggct gatctcagtc atcgaagcgg tttgcgcact aaagtcggcc
     6481 ggattggaga tgccatctcc ggtgatctca atttcatcga ccagccacca gcgcggtcct
     6541 gtaacagaaa gataatggaa tgccaggtga acattggtgc cggaaatccc tttgagatcg
     6601 ataaaaccgg aaggggtcca ggtttgttca accgacgact tggcgaatga aagttcagcc
     6661 caggtggcca gagagggatc accaccgacg taatctgtgg aatatttcag ctttaactca
     6721 ttgggatcag ttccgtagcg ccaccaggtg aagaagacca tgtattcatc ggcaaaggca
     6781 tcaaggttaa ggcttggtga gataagccag tcattctcaa gtgtatctgt ggtttggtaa
     6841 ccattaatgc cgccggattt tgcagtaccg tatgcgccgt ttgtagcgta gatcgaccag
     6901 tttcttgatt ttacggccag gctgaccgtg tcccagtcgc cgaataaccc ggattcaaag
     6961 tcgttggtgt tgacgattga ctgcgtagtg gcattgcccg ctggggccgg atcgtcggtc
     7021 aggaagtcga tacctgaacc cgaattggta tatgggaaaa ttttcaaata ataggtagtt
     7081 gttccctgaa gccggtaaaa cgtgtatttt ccaacaccct gtgcaactga aacagcccct
     7141 gttccatccg aaagatcaag atcatttgtt accggagttc catcgacagg tgctgcaata
     7201 ttatcctggc tggaaattct gatcaggtat ccatccggtg gctgtgtccc accggcatcg
     7261 gtccatgtgg ctgtgatcgt tatccctgtg tcggcaacgg caaagagcgt cgggtattca
     7321 gaaggctgta tcttcagtgt tgtggcatta tcctctgttc ctgtcgaata ttcattggcc
     7381 accgtcattg accaagcttt ataataataa gcggtgttgc tggtcaggcc ggaatggctg
     7441 aaggcaatcc ctgtcccttt ataaattacc gttccaccgc cgctgatttc agaacccaag
     7501 gaataatctc cggcaggggt tccaaaagta ttcgaaagat tagtggcgat cagtaccgaa
     7561 tcattgttgt tattcaggtt ccaggtcagg tcgatttgat aagggctgac actttctgcg
     7621 ttgaatcctg caggattaga tacgccttca gcagttacat taatcgttat atcatccagg
     7681 gcaatctcat cccttccacc ggtgcccaaa acatcatcgc tgttccagcg gaagaaaaag
     7741 ctggccccat ttgcaataga aagaccacta attagataag acctgggaac tatagtccag
     7801 ccactcgttg cagtagtagg cgtagagtaa ttgaggtcgg ttatttgaat ataggctgaa
     7861 ctgtcaatac tataactgaa attgaatgag tttgaccggg gctggtcatt gtaaacacaa
     7921 atattataag aaaaagaaat gcctgatata cttaaaccgg tgttattttt aattctcaat
     7981 gtcattgttc cgggtgtcca gtcatttcct gttggttgag ttccaaaagc ataatctccg
     8041 gttgccacct ggaatgaata aaatcctccg gtacctaagc ttacatttcc atttgagaca
     8101 cccctggcga agtctccgga tatcttcgtg tctccatacg tcgatattcc ttcgtccatt
     8161 cctgtagtgg cccatgcatc tgaatcaagc cggccggttg ccggagaagg ctccaggcct
     8221 gtaccggcaa attgtccgtt attcacgccg gttaccgtca gtggcgccgg gacatcgtca
     8281 atggtgagcg tagtcaacac gcgcgcggcg ggagagatgg tgatgaccac ctgtcgcgag
     8341 tcgccggtgg catcgccgtc aaggcga
//
LOCUS       DAOPNH010000144         8349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9477_length_8349_cov_5.692669, whole genome shotgun sequence.
ACCESSION   DAOPNH010000144 DAOPNH010000000
VERSION     DAOPNH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9477_length_8349_cov_5.692669"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /locus_tag="PKZ74_08650"
     CDS             complement(<1..2179)
                     /locus_tag="PKZ74_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92866.1"
                     /translation="MESQKIVPHRLLLVLLGFTAMATQVILIREVLAIFQGNELVIGM
                     FLGIWMILTATGAYLSVQSSKFKVQSGFLKNKSTIDNRQSTIENPIAKSPNLLFLLAL
                     LPVASLFLLVTLRFSLVPAGIMPGLGKTILVFFLALLPFCMVSGLLFPKLVNSLSLLK
                     GKNLLHEGYALESAGGVVGGLLFSLVFILILPPYESLVLLSTFCLLVLFVIWLISGKI
                     VLAILALVTGAVVFLMPTVAGIARYLDRKQFSRQDVVEIRSSPFGLLAVSKMGNELII
                     YDNGSPVSLSADVIQREEAVHYAMLLHRSPKKVLLVSGGVSGAIDEILKYPVEKVDYV
                     EPNPWLIRLVDKYRPLPDDRRIRYIYKDPLIFLRNNPGTYDVILINAAEPHSAESNRY
                     STAEFYKLLKDKLNHGGIVSVATEASGNYMNETSRMIHSVNYNTFKSAFSYIRIIPGA
                     KDYFLVSDTTIEQSIFKHYQDKGIDNEYVNPFYIDEELLRMRSELIHKDLLPDAPVNS
                     DLKPFVFSLFLRHWLEKFKMNTWIIPLILVLLLILSLIFFGPLNLGLFAGGFTASGLE
                     FILLIWFQVIYGNVYQMTGVIFAVFMLGLVIGSLLLVGGIKKNTFKGYLMIQGGYVCL
                     SLLVALLMIIMASGSPGMLTIGLILFLVLLTGLLMGTQFSSSAHLRTTSIINSARESF
                     SADLAGSATGIVLVSVYVVPLLGLPVTALFLAGLNVQRYHINRY"
     gene            2318..3565
                     /locus_tag="PKZ74_08655"
     CDS             2318..3565
                     /locus_tag="PKZ74_08655"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM92867.1"
                     /translation="MSRNKGLYFILSVILILPQIIVAQAVNPNDAEKGWKPLFNGLDL
                     AGWRGFKADGIPADWSVADGCIVVAGTGGGESANDLITAEQYEDFDLYLEWAISPGGN
                     SGIFYHVLEGDYPSTYATGPEYQLIDDIGFPGKLEEWQQAGADYAMHTADKTRKKLMP
                     VGEFNSCRIRVENGHVTHWLNGEIILEFDLWTEEWHRLKNEGKWLDYPFYGLARKGHI
                     GLQHHGSLVRFRNIKIKDLTDPGAPLFNGNDLTGWVNHGNERWFVENGMIVGSSSPDG
                     GYGYLATDREYHDFILRLQFKLEEEGNSGVFFRSLLNGTDIKGWQAEVAPSGNNTGAI
                     YESGGRGWLNEIPEEREDILKPGEWNDMVISVKGDHVQTWLNGESMTDLFDDAVGKGQ
                     GVIALQVHSGGGVAVKWRDIFIR"
     gene            3616..4239
                     /locus_tag="PKZ74_08660"
     CDS             3616..4239
                     /locus_tag="PKZ74_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92868.1"
                     /translation="MSEFYNMVHLLIGLLLLNCSGPCNSDLQSDPVINSVFSQSEIKD
                     LSIILDFFTDQICSKHLMDSANTQKCFKNYLDRMNNDGKKGVIDINIPFELQRSVYTQ
                     ISDSTLNEIWSFERSWIPHTSDTSKHIYYNPNGKYVKFLREFGEENKVIKNYYDSYVN
                     TGDISPSMVADLQMNKDVYDINDIRIQLFVAIHYLTLNDQLERKEIY"
     gene            4305..4769
                     /locus_tag="PKZ74_08665"
     CDS             4305..4769
                     /locus_tag="PKZ74_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPM92869.1"
                     /translation="MTNTQKLTLVVLRLAIGWHFLYEGLVKLANPNWTSAGYLLDSGG
                     FLKGFFISLASNPDMLKIVDLLNIWGLILIGLGLILGLFTRTAIISGIVLMAFYYLSH
                     PPFVGLKYAVPSDGSYLVINKMVIEGLALAVLLLFPTWKEWGVDRWVGRRRD"
     assembly_gap    4894..4993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5061..6590
                     /locus_tag="PKZ74_08670"
     CDS             5061..6590
                     /locus_tag="PKZ74_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM92870.1"
                     /translation="MERRDLLKSMVSVPVLGALAYAFYRKRKFEKYLRDNIQEELKID
                     TEPAALTGDPSKGREIRLGLIGYGIRGKDLALAAGFAHPTMIDDWKEGAVNNRQDKRY
                     EEYQQQEPLNVRVTAVCDIFDTYGNMAVEAGSNVHREGTGGKMGQQPKRFLNHLDLIN
                     SPEVDAVIIATPDHWHGPMTIAAARAGKHVYCEKPLTWTVEETYEVRKAVKENNIVFQ
                     LGHQGRQNESYLKAKEVISQGILGKVNLIEVTTNRNDPNGAWVYPIHETAGRQTIDWE
                     QFIGQAPWHDFSLERFFRWRCWWDYSTGLSGDLLTHEYDAMNQILGLGIPDSAISSGG
                     IYFFKDGRTVPDVLHTVFEYPGRDLTLMYSATLASNRRRGRVIMGHDGTMELGSSMTV
                     YADRGSTRFREKLDNGVIDPDLPILTYTPGLKNVDAITSPTEQYFAGRGLMYTYRGGK
                     RVDTTYLHIREWLDCIRNGGQPSCNIDQAFEEAMTAHMGTMAYKENRRVFWDPVGEKI
                     L"
     gene            6719..7567
                     /locus_tag="PKZ74_08675"
     CDS             6719..7567
                     /locus_tag="PKZ74_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM92871.1"
                     /translation="MITRRDFLKTTTLATASALLAPGILRAGPHKKDIGLQLYTVRDH
                     ISKDLPGTLRAVAKIGYTRLEAAGYNDGKFYGMAPGEFRSLVSGSGMQPVSSHVAFSP
                     EQSRHVIDAHLELDVPYVVYPWISMPEIPSADDYLRAAGLFNRLGEACKKDGLRFGYH
                     NHDFEFKKIGDTTGFDILLKNTDPDLVCFEADIYWMQYANIVPGDYLSKYPGRFELWH
                     IKDMDSGTGRTFTEVGEGTIPYGDYFTNFKDISGMKYFFVEQDKCNRDSLESTKISYR
                     NLKKIL"
     gene            7574..>8349
                     /locus_tag="PKZ74_08680"
     CDS             7574..>8349
                     /locus_tag="PKZ74_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM92872.1"
                     /translation="MGEGIINTAIVGFGLSGRVFHAPFIHTHPGFRLVTVVERHSSLS
                     KEIYPGVTIAKEYNNVLNDGSIKLVVLATPNIYHLEQARSALEAGKHVVVEKPFTPTS
                     QEADALIGIAEKTGKKIFVYQNRRWDGDFKTIQQVVYNGYLGEILEYEAHFDRYAPGA
                     RRSAWRDEPLPAGGVLFDLGSHLVDQALVLFGMPRSVYADIRMQRAESKVDDYFHVHL
                     YYERLKVILKASVFVKEQGPRYSLHGTKGSFIKYGIDPQE"
BASE COUNT         2193 a         1872 c         2163 g         2021 t
ORIGIN      
        1 ggtatcggtt tatgtggtac cgttgcacgt tcagcccggc aaggaagagc gcggtaaccg
       61 gcaatcccag caacggtacc acataaaccg ataccagcac aatccctgtt gccgatccgg
      121 ctaagtcggc actgaaggat tctctggcgc tgtttataat ggatgttgtc ctcagatggg
      181 cacttgaaga gaactgggtt cccatgagca gacctgttag cagaaccaaa aataaaatca
      241 aaccgatcgt taacatgcct ggggatccgg aagccattat tatcattaat aatgcgacca
      301 gtaaagaaag gcaaacatac cccccttgta tcatcaggta ccctttgaag gtatttttct
      361 ttataccacc aaccaataac aaagacccaa tgaccaaccc cagcataaac actgcaaata
      421 tcaccccggt catctggtaa acattgccat atatcacctg gaaccagatc agtaagatga
      481 attcaaggcc ggatgcggtg aaacctccgg caaacaggcc caggttcagg gggccgaaga
      541 agatcaatga caagatcaat aacaatacca gaatcaaggg gatgatccat gtgttcattt
      601 tgaatttttc gagccagtgg cgaaggaata acgaaaagac aaagggtttc aggtcggaat
      661 tcaccggggc atcgggcaac aggtccttgt ggatcagctc gctgcgcatt ctgagtagct
      721 cttcatcaat gtaaaaagga ttgacatatt cgttatcgat ccctttatcc tgataatgct
      781 taaagatcga ttgctcaatt gttgtatccg ataccaggaa gtagtctttt gcgccgggaa
      841 tgatccttat gtaactgaag gctgatttaa atgtattata gttcaccgaa tggatcatcc
      901 gtgaggtttc gttcatgtaa ttaccggagg cctctgtggc aactgagacg atcccgccat
      961 ggttgagctt gtctttcagt aatttataga actctgccgt ggaatagcgg ttcgattcgg
     1021 ccgaatgagg ctccgccgcg ttgataagga tcacatcgta ggtgcccggg ttatttctca
     1081 aaaaaattaa aggatcttta taaatgtacc ggatcctccg gtcatccggc aatggccggt
     1141 atttatcaac cagccggatc agccagggat tgggctccac ataatcgacc ttttcaaccg
     1201 gatacttcag gatttcgtcg atagcgcccg aaacgccgcc ggaaaccagt aagacctttt
     1261 tcggcgaacg atgcagcagc atggcgtagt gcacggcttc ttcccgttgg ataacatcag
     1321 cgcttaaact gacagggctc ccgttatcgt aaatgatcaa ctcgtttccc attttgctta
     1381 ccgccagcaa cccaaatggc gacgaccgga tctcaacgac atcctgcctg ctgaattgct
     1441 ttctgtccag gtatcgtgca atgccggcga ctgtgggcat gagaaacacc actgctccgg
     1501 ttaccagcgc taatatggcc aaaacgatct ttccggagat cagccatatt acaaacagga
     1561 ccaggaggca gaatgttgag agcagaacca ggctctcgta gggtggtaag atcaatatga
     1621 aaaccaggct gaaaagcagg ccaccgacaa cgcccccggc cgattccagg gcgtaccctt
     1681 catgcaaaag gttttttccc ttcaacaaag aaaggctgtt taccagcttt ggaaataata
     1741 aaccggatac catgcaaaat ggcaacaagg ccagaaaaaa caccagtatg gttttgccca
     1801 ggccgggcat gataccggcc gggacaaggg agaagcggag ggttacaaga agaaaaagag
     1861 aggcgacagg gagaagagcg aggaggaaga gaagatttgg cgatttggcg attggatttt
     1921 cgattgtcga ttgtcgattg tcgattgtcg atttattttt taggaagcca ctttgaactt
     1981 tgaactttga actttgaact gaaagatagg ctccggttgc tgtcagtatc atccatatac
     2041 ccaggaacat gccgataacc aattcattgc cctgaaaaat ggccaggact tcccggatga
     2101 gtatgacctg ggtggccata gcggtgaatc cgaggaggac caggagcagc cggtggggga
     2161 cgattttttg agactccatc tcaaatcaat gagactccat ctcgaaatct cgagactcca
     2221 tctcatttca aattgttcct cataaagata aattgttttc aatcgagact ccatctcaat
     2281 ttctattttt aattttaacg gtcgaaaata tggaactatg agcaggaata aaggattata
     2341 cttcatattg tcagttattt taatacttcc gcagattatt gttgctcagg cggttaaccc
     2401 gaatgatgcc gaaaagggtt ggaaacccct gttcaacggc cttgacctcg ccggctggcg
     2461 cgggttcaaa gcggatggca tacccgccga ctggtccgtt gcagacggat gcatcgtcgt
     2521 tgcgggaaca ggcgggggag aatcggccaa cgacctgatc accgccgaac aatatgagga
     2581 tttcgacctt tacctggaat gggccatttc tcccggtggc aacagcggta tcttttacca
     2641 tgtcctggaa ggggattacc cgtcgaccta tgcgaccggg cctgaatacc agttgatcga
     2701 cgatatcggg ttcccgggta aactggaaga gtggcagcag gccggggccg attatgccat
     2761 gcatacagcc gataagacca ggaaaaagct gatgccggtt ggggaattca attcatgccg
     2821 catccgggtt gaaaacgggc atgtaactca ctggctgaac ggggagatta tccttgagtt
     2881 cgacctgtgg accgaagaat ggcaccggtt gaagaatgag gggaaatggc tggattatcc
     2941 cttttacggg cttgcccgga aaggccatat cgggttgcag caccacggca gcctggtccg
     3001 ttttcgcaat attaagatca aagacctgac cgatcccggt gccccccttt ttaacgggaa
     3061 tgaccttacg ggatgggtca atcatggcaa tgagagatgg ttcgtcgaaa acgggatgat
     3121 tgtaggatcc agcagccctg acggaggcta tggctacctt gccaccgacc gggaatatca
     3181 cgattttatc ctcaggctgc aatttaaact ggaggaagag ggtaatagcg gtgttttttt
     3241 ccggtcgtta ctcaatggta ccgatatcaa agggtggcag gccgaagtgg ccccgtcagg
     3301 aaataatacc ggagccatat acgaatcggg tggccggggt tggcttaatg agattccgga
     3361 agaaagagaa gacatcctga agcccgggga atggaacgac atggtgatat ccgtcaaagg
     3421 tgaccatgta caaacctggc tgaacggtga atccatgacc gacctgttcg acgatgccgt
     3481 gggcaaaggt cagggtgtca ttgctctgca agtgcacagc gggggaggtg ttgctgttaa
     3541 atggagggat atatttattc gttagaaatt gatcgcttgt catgtgtgcg atgtccgata
     3601 gattagtcgt cagtcatgtc cgaattttat aatatggttc atttattaat aggccttttg
     3661 cttttaaatt gctcaggacc atgtaattct gacctgcaat ccgatcctgt tatcaattcg
     3721 gttttcagcc aatctgaaat caaagattta tcaataatat tagatttttt tactgatcaa
     3781 atctgttcca agcatttaat ggattcagct aatactcaaa aatgctttaa aaactatctt
     3841 gacaggatga ataatgatgg gaaaaaagga gttattgata ttaatatccc ctttgaactt
     3901 caaaggtcag tttacacaca aatcagtgat tcaactttaa atgaaatctg gagttttgaa
     3961 agatcctgga tcccgcatac atccgacacc tcaaaacata tttattataa tccaaatggg
     4021 aagtatgtta agtttttaag agaatttgga gaagaaaata aagtaattaa gaattattat
     4081 gactcgtatg taaatacggg tgatatttct ccgtcgatgg tcgctgatct tcaaatgaac
     4141 aaagatgtat atgatataaa tgatataaga attcaactgt ttgtggccat ccattacctg
     4201 actttgaatg accaactgga aagaaaagaa atatattgaa ataaccacat tataagtgat
     4261 taatgactaa tgaccaacga ccaacgacca tcaaccaaaa actcatgacg aacactcaga
     4321 agctcacact agtagttctc cgcctggcca tcggctggca tttcctctac gaaggccttg
     4381 taaaactggc caatcccaac tggaccagtg ccggttacct gctcgactcg ggtggtttcc
     4441 tgaaaggctt cttcatcagc ctggcctcca atccggatat gctgaaaatt gtcgatctcc
     4501 tgaatatctg gggattgatc ctgatcgggc tgggactgat cctgggtctt tttacacgga
     4561 ctgccattat ctccggcatt gtgcttatgg ctttttacta cctgtcgcac ccaccgtttg
     4621 tcggtttgaa atacgccgtg cccagcgatg gcagctacct ggtaatcaac aagatggtga
     4681 ttgaagggct ggcgctggcg gtgctgctgt tgttcccgac atggaaagaa tggggagtgg
     4741 atagatgggt tggaaggaga agagattgag gatattttga tattttgatt ttgtgatatt
     4801 ttgattttgt gaagttgtga tattttggta ttttgaggtt gtgatatttt gattttgtga
     4861 agttgtgata ttttggtatt ttgaggttgt gatnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnattttga ttttgtgaag ttgtgatatt ttggtatttt gaggttgtta
     5041 catactgtta ttcgggttaa atggaaagac gagatctgct gaaatcgatg gtgagtgtgc
     5101 ccgtgctggg agcgctggcg tatgcttttt accggaaaag gaaattcgaa aaatacctca
     5161 gagacaatat ccaggaggag ctgaagatcg atacggagcc ggcagcactg accggcgatc
     5221 cttccaaggg ccgggagatc aggctgggat tgatcggtta cgggatacga ggcaaagatc
     5281 tggcactggc cgcgggattt gcccacccga ccatgatcga cgactggaaa gagggggcgg
     5341 tgaacaaccg gcaggataaa agatatgaag aataccagca gcaggaaccg ctcaatgtgc
     5401 gggtgacggc ggtgtgtgat atttttgaca cgtatggcaa tatggccgtg gaagccggat
     5461 ccaatgtaca cagggaaggg acgggtggca aaatgggcca acaaccgaaa cggttcctga
     5521 accacctcga cctgatcaat tcgcccgagg tcgatgcggt gatcattgcc acgcccgacc
     5581 actggcacgg cccgatgacc attgccgcag ccagggccgg aaagcacgtt tattgcgaaa
     5641 agccgctcac atggacggtg gaagagacct atgaagtcag aaaagcggtc aaggaaaaca
     5701 acatcgtctt ccagctcggc caccaggggc gtcagaatga aagttatctg aaagcaaaag
     5761 aggtgatcag ccagggaatc ctcggcaagg tcaacctgat cgaagtgacg accaaccgca
     5821 acgatcccaa cggcgcctgg gtctatccga tccacgaaac ggccggccgg cagacgatcg
     5881 actgggagca gttcatcggc caggcaccct ggcacgactt cagcctggag cggttcttcc
     5941 gctggcgctg ctggtgggat tacagcaccg gcctgtcggg cgatctgctg acccatgagt
     6001 acgatgccat gaaccagatc ctgggcctgg gtattccgga ttcagccatt tcaagcggtg
     6061 gaatatactt ttttaaagat ggccggacag tccccgatgt gctccacacc gtcttcgagt
     6121 accccggccg cgacctgaca ctgatgtaca gcgccacgct ggccagcaac cggcgccgcg
     6181 gcagggtcat catgggccac gacggaacaa tggaactggg ttcatccatg acagtctatg
     6241 ccgaccgggg atcgactcgc ttccgggaga agctggacaa cggcgttatc gaccccgatc
     6301 tgcccatcct gacctatact ccgggcctca agaatgttga cgctatcaca tcaccaacgg
     6361 aacagtattt tgccggacgc gggctgatgt atacctatcg cggagggaaa agggtggata
     6421 ccacctacct gcatatccgc gagtggctcg actgtatccg caacggcggc cagcccagct
     6481 gcaacatcga ccaggcgttt gaagaggcga tgacggccca catgggtacg atggcgtaca
     6541 aggaaaaccg gcgcgtcttc tgggacccgg tgggggagaa gatcctttga agatttggcg
     6601 atttggcgat ttggggattt ggggatttgg ggatttgggg atttggaaat gagtcaattg
     6661 gataatttat cgtgttaatt cgttttattc gtatcatccg tgttctatta ctatttgtat
     6721 gataaccaga agagattttc ttaagaccac aaccctggct accgcttccg ccttattggc
     6781 gccgggaatc ttgagggctg gcccgcacaa aaaggatatc ggcctgcaac tgtacactgt
     6841 cagggaccac atcagcaaag acctgccggg aaccctccgg gctgtagcga agatcggtta
     6901 tacccggctc gaagcggccg gttataacga tggaaagttc tacgggatgg cccccggcga
     6961 gtttagatcg ctcgtcagcg gatcaggcat gcagccggtc agctcgcatg tggcgttttc
     7021 accggaacag agccggcatg tgatcgacgc gcacctcgaa ctggatgtgc cgtatgttgt
     7081 ttacccctgg atttcaatgc ccgagatccc ttcggccgac gattatctcc gggctgccgg
     7141 cttgtttaac cggctgggcg aagcctgtaa gaaagacggg cttagattcg ggtatcataa
     7201 tcatgatttt gaatttaaaa aaatcggcga taccactggt ttcgacatcc tgttaaagaa
     7261 taccgatcct gacctggtgt gttttgaagc tgacatttac tggatgcaat atgcaaacat
     7321 agttccgggg gattatctca gcaaatatcc cggccggttt gagttatggc atatcaaaga
     7381 tatggattcc gggaccggac ggacttttac ggaagtcggc gaagggacca ttccttatgg
     7441 ggattatttc acgaacttta aagatatttc cgggatgaaa tatttttttg tggaacagga
     7501 caaatgcaac cgcgactcgc tggaaagtac gaagatcagc taccggaacc ttaagaaaat
     7561 cttgtaaata gttatgggag aaggaattat taatacagcc attgtcgggt tcggactatc
     7621 gggaagggta tttcatgctc cctttatcca tacccatccg ggcttccgcc tggtgaccgt
     7681 tgtcgagcgg cattcttcat tatcaaagga aatctaccct ggcgttacga ttgccaaaga
     7741 gtataataat gtattgaatg atggatctat caaactggtg gtattggcaa cgcccaatat
     7801 ttatcacctt gaacaggcca ggtcagccct ggaggcgggc aagcatgtgg tggtcgagaa
     7861 acctttcact cctacctctc aggaagccga tgcactgatc gggatagctg aaaaaaccgg
     7921 aaagaagatc tttgtttacc agaaccggag gtgggatggc gattttaaaa cgatccagca
     7981 ggtggtttac aatggatatt taggtgagat ccttgaatat gaagcgcatt tcgaccggta
     8041 tgccccgggg gcgcgccgca gtgcctggcg cgatgagcca ctgccggccg gcggcgtgct
     8101 gtttgacctc ggctcgcacc tggtcgacca ggcgctggta ctgttcggga tgccgcggtc
     8161 ggtttatgcc gatatccgta tgcaacgggc agagagcaag gtggacgatt atttccatgt
     8221 acatctttac tacgagcggc tcaaagtgat cctgaaagcc tccgtttttg tgaaagagca
     8281 ggggccacgg tatagtcttc acggcacaaa gggctctttc atcaaatacg gcatcgatcc
     8341 gcaggaaga
//
LOCUS       DAOPNH010000145         8299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9544_length_8299_cov_6.651989, whole genome shotgun sequence.
ACCESSION   DAOPNH010000145 DAOPNH010000000
VERSION     DAOPNH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9544_length_8299_cov_6.651989"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2472
                     /locus_tag="PKZ74_08685"
     CDS             <1..2472
                     /locus_tag="PKZ74_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPM92873.1"
                     /translation="FPNPANGACTFQLPSSFSSKSSFEVFDISGKLIFRSEEILEPGM
                     NYFTLNGLSDGVFVIKIKTAQTVYSGKIISMNSTGNPHGLSLTRSGSSASSGGSGVHP
                     ESMKTTAAEFIMPYTAGDLLKFRGKAGLHKAVVMLVPTGDREITFNFIPCVDADGRHY
                     ATVRIGSQFWMAENLDVGVMINSSQQQTDNGIIEKYCYDNDETNGETYGSLYQWGELM
                     QYGNEGAKGLCPDGFHIPTRDEYNSMIYYLGGSTQAGGKLKERGYEHWNYPNTSATDK
                     SGFEALPNGASKGDGIFDGLKSFAKIWASSPVEPSNAWLAFLSDQSGAIGLYSYEGPE
                     GYSCRCIQDNAEEMTMLAGDTSKTWKLLRDVSTGRYPMEVGPYDHSTIWWAMGLNNDE
                     LGNRHCMLNDEWTFGRDGSLVFNAYGDYWAEGGIFDPANFCESTDYMININGEDCSGW
                     GNGNHQFALVTGDNPRLTTLGHGAYLGFYKTATDYEVTQLDPMVQDSVRYNLVMLSDG
                     ETDTLIVEVPYYFYPGDLQYGGYWRYVLVHYDNPADEPPLPGPVPTADFTAVSDGLTV
                     TCTNLSLYGEDYLWDFGDGTTDTAFNAVHTYAYDSVFHITLTVTNPNGSDMTSQDVWI
                     SEYPLTEDMLQGGPWSVRVQENSIFVGSGLGLNDWWAVPLANLDGSMIGTTDDWSCIT
                     DDEFTFGAGGSYTYQAFGDVRNDGYFGSPNGCWAESALYGNALYFASGSHTYAFTPAN
                     GTERPIITLTNGANRAAFIGFYKGFYGGENYSIENPPNGGFPTNTYEVMGYCIGEGTD
                     YLFISVDISSDHSGTAAWSVILQRD"
     gene            complement(2488..3579)
                     /locus_tag="PKZ74_08690"
     CDS             complement(2488..3579)
                     /locus_tag="PKZ74_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPM92874.1"
                     /translation="MLFKNILITGTGSYIPSAIVKNQDFADNKFYDNDGSAFDSSHDE
                     ISEKFLAITGIRERRYISDELVASDIGAKAAEIAIKDSGINREEIDQIIVAHNFGDVR
                     KHTIQTDTVPSLAARIKQILKIENPFCVGYDILFGCPGWIQGLIQAESYMRSGMAKKC
                     LVIGTETLSRVVDMHDRDSMIYADGAGACILELVESDRQEGILSHIAASYTTKEAFYI
                     YLGKGNIEEADPNIRYIKMYGRKVYEFALTHVPAAMKTCLDRAGADLRDVKKIFIHQA
                     NEKMDDEIIKRLYRMYKIHDVPKDITPMSIHTLGNSSVATVPTLYDCVRKGTGEEMQH
                     RLHEGDLILFASVGAGMNINAIAYRYSGI"
     assembly_gap    3791..3890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4025..5494)
                     /locus_tag="PKZ74_08695"
     CDS             complement(4025..5494)
                     /locus_tag="PKZ74_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM92875.1"
                     /translation="MKKTVYLLLIVMLSTLAGLSQTSDKKWNLGAYGGKSEYSGDLGN
                     GFFAISKAYYGFAALSLSRYISPRFNVAGHGSYGATGFMVYEAYPKYGAQELYGDLQL
                     QFKFVKRDKAVFKPYIFAGVGFRNLSETSSDITTANNGLDLVVPAGLGLDLRLYKNLS
                     LRYIASFGWTNNDGRDKNQTESGNDLLLHHNLGLTVNLGPAIDTDGDGVPDRKDKCPD
                     TPLGSLVDEKGCILDRDKDGIADNVDECPEIAGLAQFNGCPDTDGDGIIDSKDECPSK
                     AGLAEFNGCPDTDKDGIEDRKDDCPEVAGLASLNGCPDADGDGITDAKDDCPNDAGPA
                     ETNGCPDRDKDGIIDKLDKCPDVPGIAENKGCPEIKQETRQIFEKALQGIQFETGKDV
                     IKSNSFRILNDVVRVMEENPEYSLSIFGHTDNVGADDMNMTLSQKRAESVKRYLLEKG
                     VPESRIVEVKGFGETSPVATNDTADGRAQNRRVEFKVVF"
     gene            complement(5800..7230)
                     /locus_tag="PKZ74_08700"
     CDS             complement(5800..7230)
                     /locus_tag="PKZ74_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92876.1"
                     /translation="MKYLKIPSRPGHLRITVPDQFFHSIILILFLMTVCSCASLDSTN
                     YKSRLRAVKKTDDQSILYKVALGDVNREVKLLALSRITDQELLGKYADEMNDPGLQQM
                     AIGMITDQEILFEIALDHASSDIKMAAFHKISDMKLVNRLAVEANDPKIQLPAIGKTD
                     SQEVLFKAAMDDSRTEVRQAAFDKLTDPALIGKLAIGSGDTKLQLKAIEKVSDQNTLF
                     EIALKEDYKEAKHAAMIRLTDEQLLGKLANESKDTFFRLMVISKITDQDILYKIALED
                     ENRDAQKTALSRLSPEMLTRLVSDYDELEMQLKVIEILDDQTGLMNISQNHTDWQVRR
                     AAFTKLDDVSLDLLSREATDPAIMLGAKIRRGIISWQEAFAFKKDETGSLGNVIGAAG
                     LVDEPRPSTSDVVSACHNFIRQGDATRIPELIHLLNEFGDKSLAEDYMNCGNGELEDA
                     GVSWGRAHGYNVGSGYGSHRVRWGGR"
     gene            complement(7255..>8299)
                     /locus_tag="PKZ74_08705"
     CDS             complement(7255..>8299)
                     /locus_tag="PKZ74_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92877.1"
                     /translation="YTFFPASDQKVCLTSYKYDKKGRLIEEYRYSDNRKLINKVVITY
                     NDHGEVQEYIQSDHLGYPDFTNKYRYDDDHRLVEVEEFNSVDELACKTVNTYDDQGNL
                     TEKRLYDERGELSKKIKQEYDDSGSLVLQEEYDRGTLLMKTTRYRYNVKKILFEKILD
                     GFSMGGTFRYLYTLGAGNASTETVVFKNDKAYKMYEIQYGLSAGEVKQKLAALKSLGP
                     VNVLHDLRDEWEENKMFIINRFGKEAYQNFLEPYSIVQIELNRLFSKKSIPTTYDDLK
                     KFIETNQEIRRISDYNAPHQLDHFSRVLKVPKWKLKLGMWAGSDEMDYKEAMRSGRLL
                     IWLNALEFYEARF"
BASE COUNT         1938 a         2069 c         1958 g         2234 t
ORIGIN      
        1 ttccctaatc ccgcaaacgg ggcgtgcacc tttcaattgc cctcttcgtt ttcatcaaaa
       61 tcttcgttcg aggtttttga tatttcaggc aaattaattt tccggtcgga agaaatcctt
      121 gagcccggaa tgaattattt cactttaaac ggccttagcg acggtgtgtt tgttataaag
      181 atcaaaactg cacaaaccgt ttattcgggt aagatcatca gcatgaattc cacaggcaac
      241 cctcacggat tgagcctgac ccgttcaggc tcttccgcat catcgggcgg ttcgggagtc
      301 catccggaaa gcatgaaaac gaccgctgcg gagttcatca tgccttacac cgccggcgac
      361 ctgctgaaat tccgcggcaa agcgggattg cacaaggctg tcgtgatgct ggtgccgacc
      421 ggtgaccggg aaattacttt caatttcata ccctgcgtcg acgccgacgg acggcattat
      481 gcaacggtcc ggatcggaag ccagttctgg atggccgaaa acctggatgt cggggtgatg
      541 atcaacagca gccagcagca aaccgacaac gggatcattg aaaagtactg ttatgataat
      601 gatgagacca atggcgaaac gtacggttcc ctttaccaat ggggtgagct gatgcaatac
      661 ggtaatgaag gggctaaagg gctctgtcct gacggatttc atattcctac ccgcgatgaa
      721 tataacagca tgatatacta cctcggcggc tcgacgcaag cgggcggtaa gctgaaagag
      781 aggggatatg agcactggaa ttaccccaat acttcggcga cagataaaag tggctttgaa
      841 gcactgccaa atggcgccag caagggtgac ggcatttttg acgggctaaa atcttttgct
      901 aaaatatggg catcatcacc ggtagagcct tcgaatgcct ggcttgcttt cctgtcggat
      961 cagagcggcg ctatcggcct gtattcttat gaaggcccgg aggggtacag ttgccgctgc
     1021 atccaggaca atgccgaaga aatgaccatg cttgccggcg acacctccaa gacctggaaa
     1081 ctcctccgcg atgtgtcgac aggccgttac ccgatggaag tcggtccgta cgaccattct
     1141 acgatatggt gggccatggg gctgaataat gacgaactgg gcaaccgcca ttgcatgctc
     1201 aacgacgaat ggacctttgg ccgcgacgga tcgctggtat tcaatgcata cggagattac
     1261 tgggccgaag ggggaatttt tgatccggct aacttttgcg agagtacaga ttatatgatc
     1321 aacataaatg gcgaggattg ctccggctgg ggcaacggca atcatcaatt cgccctggta
     1381 acaggggata atcccaggct caccaccctg ggccacgggg catatctcgg cttttacaag
     1441 accgcgaccg attatgaagt gacccagttg gatcccatgg tgcaggattc tgtcaggtat
     1501 aacctggtga tgctgtcaga tggggaaacc gacacgttga ttgtggaggt accctattat
     1561 ttttacccgg gcgacttgca atacggtggc tactggcgct atgtcctggt ccattatgac
     1621 aatccggccg acgaaccgcc ccttcctggt ccagtgccaa cagccgattt taccgctgtt
     1681 tccgacggac ttacagtaac ctgcaccaat ctgtcgttgt acggagaaga ttacctctgg
     1741 gactttggcg atggaaccac cgatactgcg tttaatgccg ttcatactta tgcctacgat
     1801 agtgtgttcc atattacgct gaccgtgacc aatccgaacg gaagcgatat gacttcccag
     1861 gatgtctgga tctctgaata ccctttgact gaagatatgc ttcagggcgg gccgtggagc
     1921 gtcagggtac aggaaaactc catattcgtg ggttcagggc tgggattgaa cgattggtgg
     1981 gcggttccgc tggccaacct ggatggatcg atgatcggta cgacagacga ctggtcgtgt
     2041 attaccgatg atgaatttac atttggcgca gggggatcat acacctacca ggcttttggc
     2101 gacgtcagga atgacgggta tttcggcagt ccgaacgggt gctgggccga atcggcactg
     2161 tatggcaatg ccctctattt tgcctccgga agtcatacgt atgcctttac cccggcaaac
     2221 ggcactgaac gtcccatcat tacgctgacc aatggggcga accgggcagc atttatcggg
     2281 ttttacaaag gtttttacgg aggtgagaat tatagtattg aaaatccccc gaacggaggt
     2341 tttccgacca atacttatga agtgatggga tattgcattg gggaagggac cgattacctg
     2401 ttcatatccg tggatatctc atcggatcat tccggtacag ctgcctggtc tgttattctg
     2461 caacgggatt agaaaccggc accggcttca gataccggaa tagcggtagg cgatcgcatt
     2521 gatattcatt cccgcgccaa cggaagcgaa caggatcaga tcgccttcat ggagcctgtg
     2581 ctgcatctct tctcccgtcc cttttctgac acagtcgtac aaagtaggga ccgtggccac
     2641 ggagctgttg cccagcgtat ggatgctcat cggggtaata tctttcggga catcatggat
     2701 cttatacatc cggtaaagcc gtttgataat ctcatcatcc attttttcgt tggcctggtg
     2761 gatgaatatc ttcttaacgt ccctcagatc agctcctgca cgatctaggc aggttttcat
     2821 agccgcagga acatgggtca gggcaaattc gtatactttc cttccgtaca ttttaatgta
     2881 gcggatattc ggatcggctt cctctatatt gcctttccca aggtaaatat agaaggcttc
     2941 ctttgtggtg taggaggcgg caatatggga aaggatccct tcctgccggt cgctttcgac
     3001 cagttccagg atgcaggcgc cggcgccatc ggcatagatc atggaatcgc ggtcgtgcat
     3061 gtcaaccacc cgcgaaaggg tttcggtacc tatcaccagg cacttttttg ccatccccga
     3121 acgcatatag gattccgcct ggatcaatcc ctggatccat cccgggcagc caaataaaat
     3181 atcatatccg acgcaaaaag ggttctcaat cttcaggatt tgcttgatcc gggcagccag
     3241 gctcggcacg gtatcggtct ggatggtatg tttcctgaca tctccgaaat tatgcgccac
     3301 gatgatctgg tcgatctcct cccggttgat cccggaatct ttaatggcaa tttcagcggc
     3361 tttcgctccg atatccgaag ccacaagttc gtcggaaata taccgccgtt cccttatccc
     3421 cgtgatggcc agaaacttct ccgaaatctc atcgtgtgag ctgtcgaaag ccgatccatc
     3481 gttatcgtaa aatttattat cggcaaaatc ctgattttta acaattgcag aggggatata
     3541 actgccagtt cccgttatca ggatattttt aaaaagcata cttactggtt ctataactaa
     3601 gctacaaaat tagtaagaat ttaccttaag atattacata tcattaaagt tagttcgatt
     3661 agttttataa aaatcgggta tgggatggtc tcaaaagtcc aatattcgct ttttataggt
     3721 agtccgttga gcgtcccgtc ggttccggat gggttttcct tccgacgact aaagccgtcg
     3781 gttccggatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggttttcctt
     3901 ccgacgagcg tcccgtcggt tccggatggg ttttccttcc gacgactaaa gccgtcggtt
     3961 ccaattgtaa ctccttggaa ccaacggctt tagtcgttgg agcaaaaaat gtcaggcttc
     4021 gcttttagaa cacgaccttg aactctaccc tcctgttctg cgctctgccg tctgccgtat
     4081 cgttcgtggc cacaggcgaa gtttcgccaa agcccttcac ttccacgatc ctcgattcgg
     4141 gaacgccctt ttcgagcagg tacctcttca ccgattcggc gcgtttctgc gacagggtca
     4201 tattcatgtc gtcggcaccg acattgtcgg tatggccgaa gatcgacagc gaatattccg
     4261 ggttttcttc catcaccctg acgacatcgt tgaggatacg gaacgaattc gatttgatga
     4321 cgtctttccc ggtttcgaac tgaataccct gcagcgcttt ttcgaagatc tggcgggtct
     4381 cctgtttgat ctcggggcag cccttgtttt cagcaatacc aggtacatcg gggcatttgt
     4441 cgagtttatc gatgatcccg tctttatccc tgtcggggca gccgttggtt tcagcggggc
     4501 cggcatcgtt cgggcaatcg tctttagcat cggtgatccc gtcgccatct gcatcggggc
     4561 aaccgtttag agaagccagt ccggccactt cggggcaatc gtctttgcgg tcttctatcc
     4621 cgtctttatc cgtatcgggg cagccgttaa attcggcgag gcccgctttg gaaggacatt
     4681 catccttgct gtcgataata ccgtcgccat ccgtatccgg gcagccgttg aactgggcaa
     4741 ggccggcaat ttccgggcat tcgtcgacat tgtcggcgat cccgtcctta tccctgtcga
     4801 ggatacagcc tttctcatcc accaatgaac cgagcggtgt gtcggggcac ttgtctttcc
     4861 tgtcagggac gccatccccg tcggtatcga tggccgggcc caggttcacc gtcaggccca
     4921 ggttgtggtg cagcagcagg tcatttccgc tttcggtctg attcttgtcg cgaccatcgt
     4981 tatttgtcca cccgaaggat gcgatatacc ggaggcttag atttttgtat aacctcaggt
     5041 cgaggccgag ccctgccggg acgaccaggt cgaggccgtt attggctgtt gtaatatctg
     5101 aactggtttc ggaaaggttt ctgaaaccta cccctgcaaa tatatagggt ttaaaaacgg
     5161 ccttatctct ttttacgaat ttaaactgca actgcagatc gccatacaac tcctgggctc
     5221 cgtacttagg ataggcctca tagaccataa atccggtagc gccgtaactt ccgtggccgg
     5281 ccacgttaaa ccgggggctg atgtagcgcg acagggataa tgccgcgaag ccgtaatacg
     5341 ccttggaaat agcaaagaaa ccatttccca ggtcaccgct atattcggat tttccgccat
     5401 atgcaccgag gttccacttt ttatcgcttg tttgcgatag tcccgccagg gtgctcagca
     5461 tgacaatcaa taataagtaa actgtttttt tcatagcatt agtttttaag ttagtgatga
     5521 ttatattaat tctgtcctgt ctggacggca aaatcctttt tcctgaataa tttatcaatg
     5581 aatccgtaaa tctcaaatag atatatcccg acataaaacc tggctattat gactttcctc
     5641 aaaggtaagg ataatcaaaa cgaaatacaa catctttttt atacccttac tttaaaaggc
     5701 gcaaaattaa aagaatttct ggtgacgaag atttcaagca aagcttaaaa tatcaccctg
     5761 atgaatatcc gtaaggcaaa tctgaaaggg agaataattc tatcgcccgc cccagcggac
     5821 acggtgggat ccgtatcccg agccgacatt gtagccgtga gccctgcccc aggaaacccc
     5881 tgcgtcttcg agttcaccat tgccgcagtt catataatct tctgccaaac ttttatcgcc
     5941 gaactcgttc agcaggtgga tcagttccgg gatgcgcgtt gcatcaccct gacggataaa
     6001 gttatggcag gcggaaacaa catcggaggt tgaaggtctg ggttcatcca ccagtccggc
     6061 cgctccgatg acattgccca gcgaaccggt ctcatctttt ttaaacgcaa atgcctcctg
     6121 ccagctgata attccgcggc ggattttagc ccctagcatg atcgccggat cggtcgcttc
     6181 acgcgaaagc aagtcaagtg aaacatcatc cagcttcgtg aaagccgccc ttctcacctg
     6241 ccaatcggta tggttctggg agatgttcat cagcccggtt tgatcgtcca gtatctctat
     6301 aacttttaac tgcatttcca gttcatcgta atcggacacc agcctggtga gcatttcagg
     6361 cgacaaccgg ctgagggccg ttttttgagc atccctgttt tcatcttcca aggcaatctt
     6421 atacagtata tcctggtcag ttattttact gattaccatt aacctgaaga aagtatcctt
     6481 gctttcattg gccagcttgc ccagcagttg ctcatccgtt aaccggatca tggcagcatg
     6541 ctttgcttcc ttataatcct ctttcaaagc aatctcaaag agggtattct ggtccgatac
     6601 tttctctatt gctttaagct ggagctttgt gtcgcccgag cctattgcca gcttaccgat
     6661 cagcgccgga tcggtcagtt tatcgaatgc cgcctgcctg acctcggtgc ggctgtcatc
     6721 catggctgcc ttaaacagca cctcctggct gtctgtcttg ccaatggcgg gaagctgaat
     6781 tttcggatca ttggcttcca cagccaggcg gttaaccagt ttcatatcgc tgatcttatg
     6841 gaaggccgcc atctttatat cactactagc atgatccagt gctatttcga aaaggatctc
     6901 ctggtcggtg atcattccga tggccatttg ctgcaggccc ggatcattca tctcatccgc
     6961 atatttgcca agaagttcct ggtcggtgat tcggctcaag gcaagcaatt tgacttcgcg
     7021 gttcacatct ccaagagcga ctttgtacaa aatactctgg tcgtcagttt ttttgactgc
     7081 cctcagccgc gacttgtaat tcgtactatc aaggctggca cagctgcata cagtcatcaa
     7141 aaacaggatc aggataatgc tgtgaaaaaa ttgatccggg acggtaattc tgagatggcc
     7201 cgggcgggaa gggatcttta agtatttcat aatctatcgg tttggcaaag gttattaaaa
     7261 acgagcttca taaaattcca gtgcattgag ccagatcagc agtcgaccgc ttctcatagc
     7321 ttccttgtaa tccatttcat ccgaaccggc ccacattccc agcttaagct tccatttagg
     7381 aaccttcagc acccgtgaaa aatgatccag ctgatgaggg gcattataat cgctgatcct
     7441 tcggatctct tggttagttt caatgaactt tttaagatca tcgtatgtgg tgggaatgga
     7501 tttctttgaa aagaggcggt ttaactcaat ctgaacaata ctatagggtt ccaggaaatt
     7561 ctgataagcc tctttaccaa accggttaat gataaacatt ttattctctt cccactcatc
     7621 ccgcaagtcg tgcaggacat tgacagggcc cagggatttt aaagcagcca gcttttgttt
     7681 gacctcaccg gcagacaggc catactgaat ctcatacatt ttatacgcct tgtcattttt
     7741 gaagacaacg gtttctgtgc ttgcatttcc cgcccccaga gtatacaaat accggaaagt
     7801 gccgcccatt gagaatccat ccagtatttt ttcgaaaagg atctttttta cattgtatct
     7861 gtacctggtt gtcttcatca ataaggtacc tctatcgtac tcttcctgca aaaccaggct
     7921 gcccgaatca tcatactcct gctttatttt ttttgataat tcgcctctct catcataaag
     7981 ccttttctct gtaagatttc cctgatcatc ataagtgttc accgttttgc atgcaagctc
     8041 atccaccgaa ttaaattcct cgacttcaac cagcctgtgg tcgtcatcgt aacgatattt
     8101 gttcgtgaaa tcgggatatc ccagatgatc ggactggatg tattcctgta cttcaccatg
     8161 atcgttatag gtaatgacca ctttattgat cagcttcctg ttgtcggaat agcggtattc
     8221 ttcaatgagt cttccctttt tgtcgtattt gtatgaagtc aggcacacct tctgatcgct
     8281 ggctggaaaa aatgtatag
//
LOCUS       DAOPNH010000146         8293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9549_length_8293_cov_6.847657, whole genome shotgun sequence.
ACCESSION   DAOPNH010000146 DAOPNH010000000
VERSION     DAOPNH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_8293_cov_6.847657"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..748)
                     /locus_tag="PKZ74_08710"
     CDS             complement(98..748)
                     /locus_tag="PKZ74_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM92878.1"
                     /translation="METKLKIFIVDDHEFFRNGLKMVINRLKYARVAGEASNGREFLD
                     LLAGEEPDVVLIDIQMPVMNGIEATRLALEEYPDLKVVALTMFDDEEYIQSMIDAGAK
                     GFLLKNITKDILDQALTAVHSGKNYYSPELFDFFTRKVVSEPRFDDEEIQLTRREKEI
                     LQLICDGLSNKEIADKLFISERTVVGHKSNLLAKTNCKSSVGLLSYAIRNRLVEVR"
     gene            complement(800..2131)
                     /locus_tag="PKZ74_08715"
     CDS             complement(800..2131)
                     /locus_tag="PKZ74_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPM92879.1"
                     /translation="MITVIGLISSAFLQVVAAVSALWLLRASRAKVAWIMIFVAFMFM
                     AFRRIVEVMAITHGDISTGLILLSNWLGIIVSAIMAVAVIMIGRILYKLKKAENISHE
                     LETRFTTLFHNSSDEIYLADLKGNLIEANQVACEALGFSRQELLQKNFKDLKTPKYFD
                     TVEPNLKKIIENGRHVYETEHVSKEGKVISLEVKSRVINYMGQEAIISIARETTERKQ
                     MERKILTAVIGAEEKERERISRDLHDGLGPLLSTIKLYVNELESGDLESAEKVGMLKQ
                     TNELINEAISSTRAISNNLSPHLIMDFGLVKAVESFCKKINLVQKIRIQFSNRLDNRR
                     FDQTVEIVLYRVITELINNTIRHARASRIEIDLEIIEDMLQLTYLDDGIGFDKERVLT
                     GDSGGMGMKNIISRLRSINGNYRIHSRPGAGTLVVVEIGLNTGDSDYIPVN"
     gene            complement(2238..2441)
                     /gene="tatA"
                     /locus_tag="PKZ74_08720"
     CDS             complement(2238..2441)
                     /gene="tatA"
                     /locus_tag="PKZ74_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPM92880.1"
                     /translation="MNTFLPLLSMPSGTEWLLILAVVLLIFGGRKIPELMKGIGKGAR
                     EFKKGMSGDYDEEPKKDVPAKKE"
     gene            2527..4560
                     /locus_tag="PKZ74_08725"
     CDS             2527..4560
                     /locus_tag="PKZ74_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPM92881.1"
                     /translation="MKRFSSLFFIFLITFSVKLHAQEKQPALTHEMTPEEELRKHEIG
                     RDFTPTDPPAAPRSIAEFEEMQSVLIRYPFGIPMSLIKEMAEDCKVKTIVASAAEQQQ
                     VLSQYQSAGVNVANCEWLIAPSNSYWTRDYGPWWVADGYSDIGICDFPYNRPRPFDDN
                     IPVVLAQQMGVPLYGMNLIHTGGNWMDDGDGLAASTQLTWTENPTLTHADIDTLVKDY
                     LGINRYILQDDPLDEYIEHIDCWAKFLDVDKILVGQVPPSDYRYQDYEMIANYWAFQT
                     SAYGNLFQVYRVYTPGTYPYTPYTNSLILNKKVFVPITGSQYDDEAIEVYQEAMPGYE
                     IVPVLFGSWENTDALHCRAKGIADIGMLYVNHQPILGNKPFQMDWDLSAEIVPYSGMG
                     LISDSLRCYYKVNEGDYQSVQLEQINGYHYEATLPLVEPGSQVSYYLRAVDLSGRRKN
                     HPYIGAPDPHVFTVGYSALTVVYPDSMIYTSGDQMVTGQTFNVCNYTNSESVINDIES
                     EGFGYFHWYIEGFDMTFPYTMTVNETLPLTVKIDLPVDKAMGYMVTDTLDIYAEDGHY
                     KVVIRVDSDLLSGIANPQISSARIESIAPNPFSSETRISFSLKAEMDVTVAVCNLQGQ
                     IVRALASCEYPAGNHEITWDGKDEAGNEVRSGIYLLKLKTDRGVDYRKLIISR"
     gene            complement(4586..5539)
                     /locus_tag="PKZ74_08730"
     CDS             complement(4586..5539)
                     /locus_tag="PKZ74_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92882.1"
                     /translation="MKKNIFAFITVLTLSFSGISAQTLDEAGAAFNAGIQFAKDSSYA
                     EAIVSIEKAIEICKKIGDDGMELQIKAEQLLPGTYFNLAKSLFEQKKYNDAIPNFEKS
                     AQYADVMGETKTADASRTYLAGIYYAQGNAEYKNEALDKAIEKYNKALSYKPEYYKAF
                     YGLGLVYKKQENLPLMKENLDKAIALAGDDEKTKGNAQDAAASAYQKAGALTLQAKKY
                     TTSIENLIASQEYNSTEPRTYFYLAIAYNGLSKWDDAITAATKALELQTDDKSDIYFE
                     LAKAQENKGDKASACANYKNVTGGNNVAAAKFQIEQVLKCQ"
     gene            complement(5599..7155)
                     /gene="lysS"
                     /locus_tag="PKZ74_08735"
     CDS             complement(5599..7155)
                     /gene="lysS"
                     /locus_tag="PKZ74_08735"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPM92883.1"
                     /translation="MQFSEQEQIRRNSLQELINLGIDPYPAEGFNVSRNIHDIVAEYE
                     PVKSKYYEDGKLKDPAVEPEYEKIEVSLAGRIMNRRIMGAASFAEIQDSTGRMQIYIK
                     RDEVCPGDDKTMYNTVFKRLLDIGDIIGVKGFVFITHMGEITVHVKEYKLLSKSLRPL
                     PIVKEKEGEVFDAYADPELRYRQRYVDLIVNPEVRETFVMRNRLINSMRSFLDPKGYL
                     EVETPILQPLYGGAAARPFTTHHNSLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDF
                     RNEGMDRFHNPEFTQMELYVAYKDYEWMMDLVEEMVEKIALDLHGTSHVRVGENLIDF
                     KRPWKRFTMFEAILYYTGLDISGMEEGELRQAAAKLHVDLDPAMGKGKIIDEIFGQLC
                     EPKLIQPTFITDYPVEMSPLAKKHRSKPGLVERFEAICNSKEICNAFSELNDPIDQRK
                     RFEYQLELGKRGDQESMVLDEDFLRALEFGMPPTAGLGIGIDRLTMIMTNSPSIQDVI
                     FFPQMRPERK"
     gene            complement(7316..8104)
                     /locus_tag="PKZ74_08740"
     CDS             complement(7316..8104)
                     /locus_tag="PKZ74_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92884.1"
                     /translation="MKYLIIITWIFLSMVSAAQPGIPPRCPYKAIQFPDSRVVKEAQD
                     FTIITTDGVTRNLYNTLDSGKTVFIDIFYTTCYYCQVYAPVIEEIYQNTGAGEGDIEF
                     WGISNNLFDTNNVIDEYREQYNITNPCAGPWGDGEIAFTILVNGQNFNGFPTYCVICP
                     DRTLYFDACYPPTTTCFDPFFEQCASIGINDDIIADLQSKHKGPVTVEVYDLVGRKIF
                     SGNFESEPDLKSLSSSVQDNGMYVIRILNDNRLIDIHKIVITGR"
BASE COUNT         2082 a         1986 c         1930 g         2295 t
ORIGIN      
        1 gttaccacaa tccatgccct gcgggcaagg ggattggaaa ttatggcgaa gcttgaataa
       61 atgtttcatg gcccaccgcg tatcggttcc ggtattgtta ccgaacctcc accaaccggt
      121 tccggatagc atacgaaagt aatccgacag agcttttgca gttggttttg gccaggaggt
      181 tggacttgtg gccgacaacg gtccgttcgc tgatgaaaag cttatcggct atttccttgt
      241 tggagagccc gtcacagatg agttgcaata tctctttctc ccggcgcgtc agctgaattt
      301 cctcatcatc aaaacgtggc tcgctgacca ctttcctggt aaagaaatca aaaagttcgg
      361 gactgtagta atttttccct gaatgcacgg ctgtcagggc ctggtcgagg atatccttgg
      421 tgatgttctt taacaggaat cctttggctc cagcgtcgat catgctctga atgtattcct
      481 catcgtcgaa catggtcagt gcaacgacct tcagatcggg gtattcctcc agggccagcc
      541 gggtcgcttc gatgccgttc atcaccggca tctggatatc gatcagcacc acatccggtt
      601 cttctccggc cagcagatcg aggaactccc ggccatttga agcttcgccg gccacccggg
      661 catatttcag cctgtttatg accatcttca gcccgttacg gaaaaattcg tgatcgtcga
      721 cgataaatat tttcagtttg gtttccattt caggaaaatc gcatggaagc aaagataata
      781 ctttaagttg accggcatat cagttcaccg gaatataatc actgtctccg gtattgagac
      841 cgatttcgac caccaccagc gtcccggcgc ccggccggct gtggatcctg tagttaccat
      901 ttatagaacg aagccggctg ataatgttct tcatgcccat tccacccgaa tctcccgtta
      961 aaaccctttc tttatcaaac ccaatcccat catccaggta ggtcagttga agcatatctt
     1021 cgatgatctc gaggtcgatt tcaatccggg aagcccgggc atgcctgatc gtattgttga
     1081 tcagctccgt gatcacccgg tataatacga tctcaactgt ctggtcaaaa cgccggttat
     1141 ccagcctgtt gctgaattgg atccggattt tctgcaccag gttgatcttt ttacaaaacg
     1201 attctactgc tttaaccagg ccgaaatcca tgatcagatg gggtgaaagg ttattggaaa
     1261 tggcccgtgt acttgaaatg gcttcattga tcagctcatt ggtctgtttc agcattccca
     1321 ccttctcagc agattccagg tcgcccgact ccagttcatt cacatacagt ttgatcgttg
     1381 atagcaaagg cccaagtccg tcgtgcaaat ctctcgagat tctctccctc tccttctcct
     1441 ccgccccgat cacagccgtt aatattttac gttccatctg tttccgctct gtcgtttcac
     1501 gcgcaatcga gataatcgcc tcctgcccca tataattgat gaccctgctt ttcacctcca
     1561 gggagatcac tttaccctct ttcgaaacgt gctcggtttc ataaacatga cggccattct
     1621 caatgatctt tttaaggttc ggttccaccg tatcgaaata cttaggggtt ttcaggtctt
     1681 tgaaattctt ctgcagcagt tcctgcctgc tgaatcccag tgcctcgcag gccacctggt
     1741 tggcttcgat caggttcccc ttaagatcgg ccaggtaaat ttcatcgctg ctgttatgaa
     1801 acagggtggt aaaccgggtt tcgagttcat ggctgatgtt ttccgctttc tttaacttat
     1861 aaaggatccg tccgatcata ataacagcca cggccattat agccgaaact atgatcccca
     1921 gccagttgga aagaagaatc aacccggttg aaatatcccc gtgagttatg gccatcactt
     1981 ccacaatccg cctgaaagcc ataaacatga atgcgacaaa aatcatgatc caggccactt
     2041 tggccctgga agcccttaac agccataaag cactgacggc tgcaacaacc tgcaaaaatg
     2101 cggaagatat caggccaatt acggttatca taatgatttt gttgtgtatt ataaaaattc
     2161 aaacatacga ttaccaataa aaaaaaggct gctcagacgt atgagacagc ctccaaacgg
     2221 cttttgcctt aaatatccta ttctttttta gcggggacgt ccttttttgg ctcttcatca
     2281 taatcgccgc tcatcccttt tttaaattcc cgggctcctt tacctattcc cttcatcagt
     2341 tcgggtattt tcctgccgcc aaaaatcaaa agaactactg ccaggatcag gagccattcc
     2401 gttccggaag gcatcgataa aagaggtaaa aacgtattca tattcctgat attacaaaat
     2461 gaaaaacaaa gatacgaaat aaggcatatt aattttctaa ttttgttgaa tagaatgata
     2521 tattttatga aacgattctc atccctgttt tttatctttc tgatcacttt ttctgtgaaa
     2581 ctgcatgcac aggaaaagca gcccgcgctg acgcatgaaa tgacccctga ggaagaactc
     2641 aggaaacatg aaattggacg cgactttacg ccaaccgatc cgccagccgc accccgcagc
     2701 attgctgaat ttgaggagat gcagagtgta ctcatccgtt atccgttcgg cattccgatg
     2761 agcctgatca aggagatggc ggaagattgc aaagtaaaga cgattgttgc gagcgctgcg
     2821 gagcagcagc aggtgctcag ccagtatcaa tcggcagggg tgaatgtagc caactgtgaa
     2881 tggcttatcg caccgtcaaa tagctactgg acgcgcgact acggcccctg gtgggtggcc
     2941 gacggctatt ccgatattgg aatctgtgat ttcccttata accggcccag gcctttcgat
     3001 gataatattc ctgttgttct cgcccagcag atgggagtcc ctctgtatgg catgaacctg
     3061 atccacaccg gtggcaactg gatggatgac ggggacggac ttgctgcatc cacacaactt
     3121 acctggaccg aaaatcccac actgacgcac gccgacatcg atacgcttgt gaaagattat
     3181 ctgggtatta acaggtatat ccttcaggat gatccgctgg atgaatacat tgaacatatc
     3241 gactgctggg ccaagttcct ggacgttgat aaaatattgg tcggacaggt tcccccttcc
     3301 gattaccgtt atcaggatta tgagatgatc gccaattact gggccttcca gaccagcgct
     3361 tacggtaatc ttttccaggt ttatcgtgtt tacacacccg gtacctatcc ctacacgcca
     3421 tataccaact ccctgatcct taataaaaag gtgtttgttc ccattaccgg aagccagtac
     3481 gatgatgagg ccattgaggt ttaccaggag gccatgccgg ggtatgagat tgtcccggtc
     3541 ctttttggca gctgggaaaa tacggatgcg ctccactgcc gggcaaaagg gattgcagat
     3601 attggaatgc tctatgtaaa tcaccagccg atcctgggaa ataagccatt ccagatggat
     3661 tgggacttat cggcagagat cgttccctat tcggggatgg ggttgatcag cgattccctg
     3721 cggtgttatt ataaagtaaa tgaaggggat taccagtctg ttcaactgga gcagattaat
     3781 ggatatcatt acgaagccac tctccctctt gttgaaccgg gcagccaggt gtcgtactac
     3841 ctgcgtgccg ttgatctttc gggaagaaga aagaatcacc cttatatcgg cgcacccgat
     3901 ccgcatgttt tcacagtagg gtattccgct ttaactgtgg tttatcccga ctccatgatc
     3961 tatacctccg gcgaccagat ggtaaccggc cagaccttca atgtttgtaa ttataccaat
     4021 agtgaatcgg tgatcaatga tattgaaagc gagggatttg gatatttcca ctggtatatt
     4081 gaaggatttg acatgacatt tccgtatacg atgactgtga atgaaaccct gccgctaacg
     4141 gtaaagatcg atcttcccgt tgataaggca atgggatata tggtaaccga tacgctggat
     4201 atttatgctg aagatgggca ttacaaggtg gtgataagag tagatagcga tctgctgagc
     4261 ggtattgcca atcctcagat ttcttctgca agaattgaat cgatagcacc caatccgttc
     4321 agcagcgaga ccaggatctc tttcagcctg aaagcagaga tggatgtaac ggttgctgta
     4381 tgtaacctgc aggggcagat tgtcagggcc ctcgctagct gtgaataccc ggcaggaaat
     4441 catgaaatta cctgggatgg gaaagatgaa gcagggaatg aagtacgttc aggaatttac
     4501 ctgttgaaac tcaagacgga cagaggagtt gattaccgga agctaattat ttccagatag
     4561 cagatattct attaattttt agttcttact ggcatttcag cacctgctcg atctggaatt
     4621 tggcagcagc gacattattt ccgccggtga cattcttgta gttggcacat gcagaggctt
     4681 tgtcgccctt attctcctgg gctttagcca gttcaaaata gatatccgat ttgtcgtcgg
     4741 tctgaagctc cagggctttg gttgcagcgg tgatggcatc atcccatttg gaaagcccgt
     4801 tgtaagcgat agccaggtag aaataggtcc ggggttccgt actattgtat tcctgggaag
     4861 cgatcaggtt ttcgatcgac gtcgtgtatt tcttggcttg cagggtaagc gcaccggctt
     4921 tctggtaagc tgaagcagca gcatcctgcg cgtttccttt ggttttctca tcgtcgccgg
     4981 caagtgcgat ggctttgtca aggttttcct tcatcagggg aaggttttcc tgctttttgt
     5041 aaaccaggcc aaggccgtag aaagctttgt aatattcagg tttataggaa agggccttgt
     5101 tgtatttttc gatggctttg tcgagggctt cgttcttgta ttcggcattt ccctgggcat
     5161 aatagatacc ggccaaatat gtcctgctgg cgtcggcagt tttcgtttca cccatcacat
     5221 cagcatactg cgccgacttt tcaaaatttg ggatcgcatc attgtatttt ttttgttcga
     5281 acaagctttt agccagatta aaatatgtgc cgggaagtag ctgttcagcc ttgatctgga
     5341 gttccattcc atcgtcgcca atctttttac aaatttcaat tgccttctca atagaaacaa
     5401 ttgcttcggc ataggatgaa tcttttgcaa attggatgcc tgcattgaag gcagcgccgg
     5461 cctcatcgag agtctgggcg gaaattcctg aaaaactgag agttaaaacg gtaatgaatg
     5521 caaaaatgtt ttttttcata gggatcatat ttaaaatgtt gattatcaaa tcgggtgcca
     5581 aaggtagaat atttctaatt atttcctctc gggccgcatc tgggggaaga atattacatc
     5641 ctgtattgac ggagaattgg tcatgatcat cgtgagccgg tcgatcccga tgccgaggcc
     5701 tgcggtcgga ggcataccga actccagagc cctgaggaag tcttcatcca gcaccatcga
     5761 ttcctggtcg cctcttttgc caagctccag ctggtattcg aagcgcttcc gctggtcgat
     5821 cgggtcgttc agctcggaaa aagcattgca gatctccttg ctgttgcaga tggcttcgaa
     5881 ccgctctacc agcccgggtt tgctgcggtg cttcttagcc agcggcgaca tctccaccgg
     5941 gtaatcggtg atgaacgtcg gttggatcag tttcggttcg caaagctgcc cgaatatctc
     6001 atcgatgatc ttacctttgc ccatggccgg atcaagatcg acgtgcagtt ttgcagccgc
     6061 ctgccgcagc tctccttctt ccattccgct gatatccagg cctgtgtagt acaggatagc
     6121 ttcgaacatt gtgaaccgct tccaggggcg tttgaaatcg atcaggttct cacctacccg
     6181 tacatgtgag gttccgtgaa gatcgagcgc gattttctcc accatctcct ccaccagatc
     6241 catcatccat tcataatcct tatacgccac atacagctcc atctgggtga actcggggtt
     6301 atgaaaacgg tccatccctt cgttgcggaa atctttcgaa aattcataaa cgccatcata
     6361 cccgccgacg atcagccttt tcaggtaaag ctcattggcg atgcggaggt agagggtcat
     6421 atcgagcgaa ttgtgatgcg tcgtgaaagg ccgggcggca gctccaccat acagcggctg
     6481 caggataggc gtctctactt ccagataacc tttcggatcg agaaacgaac gcatcgagtt
     6541 gatcagccgg ttacgcatga caaaggtttc cctgacttcc gggttaacga tcaggtcgac
     6601 ataacgctgg cggtaacgga gttccggatc ggcatacgca tcgaacactt cgccctcttt
     6661 ttctttcaca atgggaagag gcctgagcga tttggaaaga agcttgtatt ccttcacatg
     6721 gacggtgatc tctcccatat gggtgataaa gacaaagcct ttcacgccaa tgatgtcgcc
     6781 aatatccagt aaacgcttga acactgtatt gtacatggtc ttgtcatcgc cagggcaaac
     6841 ctcatccctt ttaatataaa tctgcatcct tccggtcgag tcctgtattt cggcaaagga
     6901 agcggcaccc attatgcgcc tgttcataat gcggccggcc aggctcactt caattttttc
     6961 atattcaggc tcaacggcgg gatctttcag cttgccgtcc tcgtaatatt tgcttttcac
     7021 cggttcgtat tcagcgacga tgtcatgaat attcctgctc acgttaaatc cttcggcagg
     7081 ataaggatca atgcccaggt tgatcagttc ctgaagcgaa ttacggcgta tttgttcctg
     7141 ttcggaaaat tgcattgcta aattgctaaa ttgttaaact gtttaaatgg atctctatat
     7201 cgtttgaatc gcttgttttt tttgtagtcc gacgactaaa gccgtcggtt caacctgaag
     7261 tcagcagtcc gacggtgcaa agataggaaa ctatcggtaa aaacgttttg gcgacttatc
     7321 tcccggtaat cacaatctta tggatatcga tcaaccggtt atcattcagt atcctgataa
     7381 catacattcc gttatcctga acagaagatg aaagagattt cagatcgggc tccgattcga
     7441 aatttcctga aaagattttt ctcccaacaa gatcataaac ttcgacagtc acagggccct
     7501 tgtgttttga ctgaagatca gcaataatat catcattaat tccgatgctg gcgcattgct
     7561 caaaaaacgg atcgaagcag gtagtggttg gcggataaca ggcatcgaaa tacagggtcc
     7621 ggtcgggaca gatgacgcaa tacgtcggga atccgttgaa gttctgcccg ttgacaagga
     7681 tcgtaaaggc gatctccccg tcgccccagg gccctgcgca cggatttgta atgttgtatt
     7741 gctccctgta ttcgtcgatc acattatttg tatcaaacag attgttggag ataccccaga
     7801 actctatatc tccttctccg gcaccggtat tctggtagat ttcttcgata accggggcat
     7861 agacctggca ataataacaa gtggtataaa aaatatcgat gaaaacagtt ttaccggaat
     7921 ccagggtatt gtagaggtta cgggtgacac cgtcggtggt gataatggtg aagtcttggg
     7981 cctcttttac cacgcggctg tcggggaact gaatggcttt ataggggcag cggggaggaa
     8041 ttccgggctg cgcggcagaa accattgata aaaatatcca agttatgatg atcaaatatt
     8101 tcacttatat atatttttag atgcaaatgt aaacataatc tatatgatga gatttgttat
     8161 tcaccacaga gttacacaga ttttcacaac ttgtcccgca aggcgggaga gtaatacaga
     8221 gtattttgat catatttaat taacaatagg acagttggac agcacgtcct gagggcgaag
     8281 cccgaaggat cac
//
LOCUS       DAOPNH010000147         8274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9581_length_8274_cov_6.065093, whole genome shotgun sequence.
ACCESSION   DAOPNH010000147 DAOPNH010000000
VERSION     DAOPNH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9581_length_8274_cov_6.065093"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="PKZ74_08745"
     CDS             <1..1789
                     /locus_tag="PKZ74_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPM92885.1"
                     /translation="TYPGLIPYPEGKTTGIATVLQTAQTPYAWEVETFTSAAATDLVI
                     YELLIRDFTSQRSYAALTDTLGYLERLGVNAIELMPVNEFEGNISWGYNPSFYFAPDK
                     YYGPKNELKRFVDECHKRGIAVFIDLVLNHSYDQSPMVQMYFDGNKPTGLNPWYNVDH
                     NFTNTGAQWGNDFNHESPETQAFVDSVNSYWMSEYKIDGFRFDFTKGFGNNIKLSDDE
                     WGSKYDADRVRLLKRMAAEIWERNHNAVVIFEHLAENLEEKELADYGILLWGNLNYNF
                     AEAAMAYHTNNKSDFGWISYQKRTWNDPHLVGYMESHDEERIAYKCQQWGQEKDWYNI
                     REHGIMLRRLAMNAAFLLTIPGPRMIWQFGELGYDYSIDYNGRTGEKPVRWDYYSDYF
                     RKYLYDFYSAMIRLRTTHPAFETDDFTLSVSSEMKKVVLEHPDMDVVLIGNFDVINGE
                     IIPGFTHTGTWYEYFTGQTLEVTDVSAPVMLKHGEYRLYTDVPLETPQIGTGIGQIDI
                     NGSRLLNVWPNPSNNFSIDVTLNNPAKMNISIYDVSGQLVDHVYSGQLQSGQSTFIWS
                     ARQAQVNPGIYFCKASTNYRAEISKIVVK"
     gene            complement(2176..4407)
                     /locus_tag="PKZ74_08750"
     CDS             complement(2176..4407)
                     /locus_tag="PKZ74_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92886.1"
                     /translation="MKTKRWKKGMVVMIILLAGWHTPARPGIIVTGILDGTLTGGSPK
                     AVEIFVTGTENLNNYEVWRSLNGAPFGSGSGSVASLNGVFTNTFVYLVKTDHVNAFHD
                     VFGNEGIFANVVPMGIINGNGNDGFQVRKKVGSVVIDQVWLEDASYSYEDSYWYRKNG
                     TGPDGGWIPSAWETPGNGALDGLDQEGLRAAVPFGTYASVWLGINSAWNDSVNWSLGY
                     VPTSQTNVVIPDSLTEYPLIFAPPLEPATCLNLFIAENARLTVESSACFTISGNMEIE
                     SPDTTGALQGLILSSDPNPEMSGSLIVKGTAVGKVTVRRYIPKNNNWHLIASPADTQY
                     FQPEFVPVPVDQSFDLYYWNETAEFNSGWINCRDENGGWYPGFETGFHEGKGYLAAYS
                     PATGGNEVRRFYGRIRTGDYSFPLSCSGNAWNLLGNPYTCALDWASAGISKESIAGSA
                     VYLWDPYLNSGQGGYRAHNGTTGVPSGTSPSIPAMQGFFVNALVAGTIVIDTDNDAPL
                     IHGDHDYYKETPALTANQVRLRISNASGSDEALVYFDPQATNQYDPLLDATKLFGLPD
                     CCPEIFTLTGDNHSLCINLLNEVPVSIPIGISFPSEDYLVVSAFGHEFFDPGTDIFLE
                     DLMTGTWTDLGENPDYGFFHDPDAPGIRFMLHFSGSLSQDEPNNMELPDLQITGNLIL
                     IRNPKALKGMVETIAPDGRQLYREPLKGGEYSLQAEPVTPIYIVRIITNAGTQVRKAI
                     YQP"
     gene            complement(4572..4913)
                     /gene="hypA"
                     /locus_tag="PKZ74_08755"
     CDS             complement(4572..4913)
                     /gene="hypA"
                     /locus_tag="PKZ74_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPM92887.1"
                     /translation="MHELSIAMSIVELAGEEAAKAGASEISKIEVEIGSLAGIETDAL
                     LFCWDAAIRDTMACRAALVIHPIDAEAHCLDCGHVFPINDYYSGCPSCQGFQYRITKG
                     RELRIRSLTVE"
     gene            complement(4917..5423)
                     /locus_tag="PKZ74_08760"
     CDS             complement(4917..5423)
                     /locus_tag="PKZ74_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92888.1"
                     /translation="MKKLSFSLAGLVIFLLMINGCDKESSSAGISGKITGHSDCKFSR
                     SSGISYELADTLSCVYYLYNEISGELNIIHVNSGFNCCPGELSCEITISQDTILIREY
                     EKEQACDCNCLYDLYITVKGVYMGNYVILFNEPYAYGMEELIFPINLAENISGSYCVA
                     RKNYPWGI"
     gene            5541..5789
                     /locus_tag="PKZ74_08765"
     CDS             5541..5789
                     /locus_tag="PKZ74_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPM92889.1"
                     /translation="MCLAVPGKLISIETDTPLKMGKVDFDGVVTDICLEFLPEANVGD
                     YVLAHVGTAISLLDEEEAMETLRALRELGEIKPDYELQ"
     gene            complement(5808..6908)
                     /locus_tag="PKZ74_08770"
     CDS             complement(5808..6908)
                     /locus_tag="PKZ74_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92890.1"
                     /translation="MKNLFLASVICLLFSCSPGQNPGQDNAGKVNLADSLRYPDEKHL
                     ANLRQLTFGGDNAEAYFSFDDKRIAFQSNNAAWNLECDQIFTFELASGDMKEGAPQMI
                     STGMGRTTCSYFMPGDTTILFASTHAGDHACPHVPPPRPDHKYVWPIYDDYDIYVSDL
                     KGNIIDTLTHEPGYDAEATVSPKGDKIVFTSLRSGDLELYTMNLDGSDVRQITDELGY
                     DGGAFFSQDGSMLVFRASRPKTEEDIRVYKELLSVGQVMPTNMEIFVCNADGSNLRQV
                     TNLGKANWAPYFHPSMKKVIFSSNHASDRGYQFNLYMINLDGTGLEKITYDGVFDAFP
                     MFSFNGKKLIFSSNRNNGGTRATNLFIADWID"
     gene            complement(7007..>8274)
                     /locus_tag="PKZ74_08775"
     CDS             complement(7007..>8274)
                     /locus_tag="PKZ74_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92891.1"
                     /translation="QVILKGNATSLPQLVTSGFSVNYSVPGNTVSTNKTNFWDYEDQL
                     FGVNLPPNIGLTGVGLSGTMAVATPENDHFFVDGIPVTPYQDNDLVNEDPYQLALVEL
                     HDNQNNLIVQAQPVIPVSNEINCVSSGCHSSEAQILNMHEDEGGFDPNDTPILCAECH
                     SSNALGTPGMPGLESLSQVIHEQHGEETNDCYKCHPGPNTQCHRDIMHTMGMVCQDCH
                     GSVDEVGQSIDDGREPWLEEPQCGNTDCHGSNYAEEQGKLFRQSRGHGGLFCSACHGS
                     PHALYTSENDRDNQQIIALQGYAGILRECSVCHGVTPAFPGPHGYIPTSVDQLEGEVK
                     ESLLLSVPQPNPFKERTVISFNIDKTGKTYLDIFDQNGKQMVRLVSENLGKGQYQITF
                     NAPGFAPGVYILNLRNNGRESSQKLVLTR"
BASE COUNT         2078 a         2054 c         2026 g         2116 t
ORIGIN      
        1 gacctacccg gggctgatcc catacccgga agggaaaaca accggcatag ccaccgtcct
       61 gcaaaccgcc cagacgcctt atgcctggga ggtggagacg tttacctctg ccgccgccac
      121 cgacctggtc atctatgaat tgctgatccg tgatttcacg tcgcagcgct cctatgcagc
      181 cctgaccgat acgctgggct acctcgaacg gctgggcgtc aatgctattg aactgatgcc
      241 ggtaaatgaa tttgagggca acataagctg gggctataat ccgtcgttct atttcgcccc
      301 cgacaaatac tacgggccga agaacgagct caaaaggttt gttgatgaat gccacaaaag
      361 agggatcgcc gtctttatcg acctggtgct gaaccattcc tacgaccaga gcccgatggt
      421 gcagatgtac ttcgacggca acaaaccgac aggcctgaat ccctggtaca atgtcgacca
      481 taactttacc aacaccggag cgcaatgggg aaacgacttc aaccacgaaa gccccgaaac
      541 ccaggcattt gtcgatagtg ttaatagcta ttggatgtcg gaatacaaaa tcgacggatt
      601 ccggttcgat tttaccaaag gtttcgggaa taatatcaaa ctttcggatg atgagtgggg
      661 gagcaaatac gacgccgacc gggtcaggct gctgaaaagg atggctgcag agatctggga
      721 acgtaatcac aatgcggtgg tgatcttcga acacctggcc gagaacctgg aagagaaaga
      781 actggctgat tacggcatcc tgttatgggg gaacctgaat tacaactttg ccgaggccgc
      841 tatggcctat cataccaaca acaaatccga ctttggctgg atcagctacc agaaacgcac
      901 atggaacgac cctcacctgg tggggtatat ggagagccac gatgaggaga ggatcgccta
      961 caaatgccaa caatggggac aagaaaagga ttggtacaat atccgggagc acggcattat
     1021 gctgcggagg ctggccatga atgccgcttt cctgcttact attcccggtc cgcgcatgat
     1081 ctggcagttc ggtgaactgg gctacgatta ttcgatcgac tataacgggc ggaccggaga
     1141 gaagcctgtc cgctgggatt attattctga ttacttcaga aagtatcttt atgattttta
     1201 cagcgccatg atccggctca ggactaccca cccggcgttt gagacggatg atttcaccct
     1261 gtcggtatct tcagaaatga aaaaggtggt gttggaacac cccgatatgg acgtggttct
     1321 gataggcaat ttcgatgtga tcaacggcga gatcatccct ggttttaccc acaccggaac
     1381 ctggtacgaa tattttaccg gtcaaacgct ggaggttacc gacgtgtcag cccccgtcat
     1441 gctgaagcat ggcgaatacc gcttatatac cgatgttccg ctcgaaacgc cccagatcgg
     1501 cacggggata ggccagattg atattaatgg cagtcgattg ctgaatgtct ggccaaatcc
     1561 ttcaaataac ttttcgatag atgtcaccct caataacccg gcaaaaatga atataagcat
     1621 ttacgatgtt tcggggcaat tggtggacca tgtctacagt ggacagctgc aatcgggcca
     1681 atctactttt atatggtctg cccgccaggc gcaagtcaac ccggggatat atttttgtaa
     1741 agcatcaaca aattatcggg cagaaatctc caaaattgtt gttaagtgat cacgaattcc
     1801 gcgaattaaa cgaatttcca cggataaaga ggataatagc ttcaatacgg taaaaccgga
     1861 caagcatccc ataataatcc gtttcaatcc ctgagattcg tttcattcgc gttcccgggg
     1921 ggtagtttca ttattctgaa gctttattct ttcgtatggc tccagccatc ttataaattg
     1981 cataggcgaa tactccgagc aacatttcta ttaaccctcc atcgatgggc gcaaagcctc
     2041 cgggatttcc gttcgacccg tgttgttcag gtggcatggg aggttggcca taaacgcagg
     2101 caattactgt taacatcaag ataatgaatg atatgatgat cctgaaattt attcttctat
     2161 tcatgttaat ttgtattaag gttgataaat tgcttttcga acctgggttc cggcatttgt
     2221 gatgatcctc acaatgtaga ttggcgttac cggttctgcc tggagactat actcaccgcc
     2281 cttcagtggc tcccggtaga gctgcctgcc atccggagcg atggtttcca ccattccttt
     2341 cagcgctttc ggatttcgga tcagaatcag gtttccagtg atttgcaaat caggtaattc
     2401 catgttattc ggttcatcct ggctgagcga tccgctgaaa tgcagcatga agcggattcc
     2461 cggtgcatca ggatcatgaa agaatccata gtccggattt tctcccaggt cggtccaggt
     2521 accggtcatt aaatcttcca ggaaaatatc tgtacccgga tcaaagaatt catggccaaa
     2581 agcagacacc accaggtaat cctctgaagg aaatgaaatc cctattggaa ttgaaacagg
     2641 tacttcattc agcaggttaa tgcacagcga atggttatcg ccggtgaggg tgaatatttc
     2701 gggacaacag tcaggcaagc cgaacagttt ggttgcatcc aggagcggat cgtattggtt
     2761 cgtagcctgt gggtcaaagt agacaagggc ttcatcggag ccggatgcgt tgctgatacg
     2821 gagccttacc tggttggccg taagcgccgg cgtttccttg taataatcgt ggtcgccatg
     2881 gatcagcggg gcatcattgt cggtatcaat gacaattgta cccgccacca gtgcattgac
     2941 aaagaatccc tgcatggccg gtatggaagg gctggtgccc gaaggaacgc cggttgtgcc
     3001 attatgagcc cggtatccac cctgtcccga gttcaggtat gggtcccaca ggtaaacggc
     3061 acttccggcg atggattctt tgctgatccc tgccgaggcc caatccaggg cgcaggtata
     3121 cggattgccg agaaggttcc aggcattccc cgaacaactc agcgggaaac tgtaatcacc
     3181 tgtcctgatc cggccataaa accggcggac ttcgttgccg ccggttgcag gtgaataggc
     3241 ggccaggtac cctttacctt catgaaaccc ggtttcgaat cccgggtacc agccgccatt
     3301 ttcatccctg cagttgatcc accccgagtt aaattccgct gtctcgttcc agtagtacag
     3361 atcgaaactc tggtcgaccg ggacggggac aaattcgggc tggaaatatt gtgtatcggc
     3421 cggcgacgcg atcagatgcc agttgttgtt tttcgggatg taccgcctga cagtgacttt
     3481 tcccacggcc gtacctttca cgatcagtga tccgctcatt tcggggttgg gatcggagct
     3541 caggatcaat ccctgcaaag cacccgtcgt atcagggctt tcgatctcca tgttgccgga
     3601 aatggtaaag caggcggagg attctaccgt cagcctggca ttctcagcta taaacaggtt
     3661 aaggcacgtg gccggctcga ggggaggggc aaagatcagc ggatattcgg ttaacgaatc
     3721 gggaatgacg acgttggtct gagaagtggg gacatatccc agcgaccagt tcacgctgtc
     3781 gttccacgcc gaattgattc ccagccagac acttgcataa gtaccgaaag ggaccgcggc
     3841 ccggagtcct tcctggtcca acccgtccag cgcaccattt ccaggcgttt cccaggcgga
     3901 tggaatccac ccgccgtcgg gcccggtccc gtttttccga taccagtagc tgtcttcgta
     3961 agaataagaa gcatcttcaa gccagacctg gtcgatcacg accgaaccga ctttcttcct
     4021 cacctggaac ccgtcgttgc cattcccgtt gataatcccc atcggcacca cgttggcaaa
     4081 gatcccttca ttcccgaaaa catcatgaaa tgcattaaca tgatctgttt taaccaaata
     4141 aacaaaagtg tttgtaaaca caccatttaa agaagcaacg gacccggaac ccgatccgaa
     4201 cggcgcaccg ttgagcgacc gccacacttc gtagttgttc aggttttccg tccccgtaac
     4261 aaagatttcg accgctttcg ggctaccgcc cgtgagcgtc ccatccagta tgccggttac
     4321 tatgatcccc ggcctggccg gagtatgcca tcccgccagc aaaatgatca tcacgaccat
     4381 tccctttttc catctttttg ttttcataac cgttttttta tgtttatccg gtttttggat
     4441 gaaaaatacc cggtcgatcc gaaaaatatt tttgggaggg gtataactat ataattaaca
     4501 gtgatttgga ggatcctgca ggaaagccaa aaatcatttt tttgtccgga agataagact
     4561 cagagtaagt tttattcaac ggtgagcgat cggatacgca actctcttcc tttggttatc
     4621 cggtattgaa acccttgaca ggaagggcat cctgaataat aatcattaat tggaaaaaca
     4681 tgtccgcaat ccaggcaatg cgcctctgca tcaataggat gaatgacgag ggcggcccgg
     4741 catgccattg tatccctgat cgcggcatcc cagcagaata gaagggcgtc tgtctcaatc
     4801 ccggccaggg agcctatttc cacttcaatt ttcgatattt cggatgcacc ggcctttgcg
     4861 gcttcttccc cggccagttc gacaatgctc atcgctatgg aaagttcatg catccctcaa
     4921 atcccccacg gatagttttt cctggccaca caatatgaac cgctgatgtt ttccgccaaa
     4981 ttaatgggaa agatcagctc ctccatgccg tatgcataag gctcattaaa aaggatcaca
     5041 taattcccca tatacacacc ctttacggtg atatagagat cgtataggca gttgcaatca
     5101 caagcctgct ccttttcata ttcacggata agaatagtat cctgactgat ggtaatttcg
     5161 catgacaatt ctccggggca acagttaaac ccgctgttaa catgaataat gttcaattca
     5221 cctgaaattt cgttgtacag gtaataaacg cagctcaggg tgtcggccaa ttcatacgat
     5281 attcccgatg atctggagaa tttgcaatcc gaatggccgg tgatttttcc ggatattccg
     5341 gccgatgagg attctttgtc gcatccgttg atcatcaata aaaagatgac caatcctgcc
     5401 aggctgaagg atagcttttt catgactcta atcgattttg gctctaatca gaacattgat
     5461 gtaaaattac aattaaagga agtggattta taacagaaat ttatggactt ttgcagaaat
     5521 tctattaata ggaaactgaa atgtgtctgg ctgtcccggg aaaactgatc tcgatagaaa
     5581 ccgatacccc gttaaagatg gggaaagtcg attttgacgg tgttgtaacg gatatttgcc
     5641 tggaattttt acctgaagcc aatgtggggg attatgtcct ggcccatgtc gggacggcga
     5701 tcagccttct cgatgaagaa gaagccatgg agaccctgcg ggctttgcgc gaattaggtg
     5761 agatcaagcc cgattacgaa cttcagtaaa gtgcggtttc aaatctccta atcaatccag
     5821 tcggcgataa aaaggttcgt tgcccttgtt cctccgttat tcctgttgga ggagaagatc
     5881 agttttttac cgttgaagga aaacatcggg aaagcgtcga aaacgccgtc atacgttatc
     5941 ttttcaagtc ctgttccatc caggttgatc atgtaaagat tgaactggta gccccggtcg
     6001 gacgcatgat tggagctgaa gatcactttc ttcatggagg ggtggaaata gggggcccag
     6061 ttggctttgc caaggtttgt cacctgcctc agattcgaac cgtcggcatt gcagacaaat
     6121 atctccatat tcgtcggcat aacctgccct actgaaagaa gctctttata aacacggata
     6181 tcctcctccg tttttggccg tgaagccctg aataccagca tgctgccatc ctgcgagaag
     6241 aacgcaccgc cgtcgtagcc aagctcgtcg gtgatttgcc tgacgtcact gccatcgagg
     6301 ttcatggtat aaagctccag gtcgccggag cgaagggaag taaagacgat cttatcgcct
     6361 ttgggcgaaa cggtcgcttc ggcgtcatat cccggttcgt gggtcagcgt gtcgatgata
     6421 ttgcctttca ggtcggaaac atagatgtcg taatcgtcat agatgggcca gacatacttg
     6481 tgatcgggcc tgggtggcgg aacatgcgga caggcatgat cacccgcatg ggtcgaggca
     6541 aacaggatgg tcgtatcccc gggcatgaaa tacgaacagg tcgtccgccc catgccggtg
     6601 ctgatcatct ggggtgcgcc ctctttcata tcgccggatg ccagctcgaa agtgaagatc
     6661 tggtcgcact ccaggttcca tgccgcgtta ttcgactgaa aagcgatcct tttatcatcg
     6721 aaactgaaat aggcttcggc attatctccc ccgaaggtga gctgcctgag atttgccagg
     6781 tgtttttcat cagggtaccg cagcgaatcg gccagattaa ccttgccggc attatcctgg
     6841 ccgggatttt ggcccggact gcaagaaaat agcaagcata tcacgcttgc taaaaataaa
     6901 ttcttcatat ttaattattg aaatgcaaaa ctacaaaatt ccgcattaga gggaattgta
     6961 attatttagg aggatgttcc agaattcagc ctttctcttc ttgtttttat cttgtcagta
     7021 ccagcttctg cgatgattcc ctgccgttat ttctcaggtt caggatataa acccccggtg
     7081 caaatcccgg cgcattaaaa gtaatctggt attggccttt tccaagattt tcgctgacca
     7141 gtcgcaccat ttgttttcca ttctggtcaa atatgtccag gtatgttttt cctgttttgt
     7201 caatattgaa agaaatcaca gtacgctctt taaaaggatt cggctgggga acggaaagta
     7261 aaaggctttc tttaacttcg ccttcaagct ggtcgacaga ggtaggtata tagccgtgcg
     7321 gaccgggaaa tgccggtgtt acaccatggc aaacgctgca ctctctcaag ataccagcat
     7381 agccctgaag ggcgatgatc tgctggttgt ccctgtcatt ttcactggta taaagggcgt
     7441 gcggtgatcc atggcaggca ctgcagaaca ggccaccatg tcctctcgat tgcctgaata
     7501 gcttcccctg ttcctcagca taatttgagc catgacagtc agtgttgccg cattgtggtt
     7561 cctccagcca gggttccctg ccgtcatcaa tcgactgccc gacttcatcc acgctgccgt
     7621 ggcagtcctg gcaaaccatt cccattgtgt gcatgatgtc gcgatggcat tgtgtgtttg
     7681 gccccgggtg gcatttgtag cagtcgttgg tctcttcccc atgttgctca tggatcactt
     7741 gcgaaagcga ttcaagtccc ggcattccag gtgttccaag tgcattggaa gaatggcact
     7801 ccgcgcaaag aatcggtgta tcattcggat caaatccccc ttcatcttcg tgcatgttca
     7861 ggatttgagc ctcactgctg tggcagcccg aactgacaca atttatttca tttgagaccg
     7921 ggatgaccgg ttgcgcttgc acaataagat tgttctgatt atcatgtaac tccaccaggg
     7981 caagctggta aggatcttca ttcaccaggt cgttatcctg gtaaggcgtt acgggaattc
     8041 catccacaaa aaaatgatcg ttctcaggcg tagccacggc cattgtaccg ctcagcccca
     8101 cacctgtcag cccgatattg ggagggagat tgacgccgaa caactgatct tcgtagtccc
     8161 agaaattcgt cttatttgtt gaaacagtat ttccaggcac agaataatta acactgaaac
     8221 ctgatgtaac cagctgggga aggctggtag cattgccttt aaggattacc tggg
//
LOCUS       DAOPNH010000148         8267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9593_length_8267_cov_6.625670, whole genome shotgun sequence.
ACCESSION   DAOPNH010000148 DAOPNH010000000
VERSION     DAOPNH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9593_length_8267_cov_6.625670"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKZ74_08780"
     CDS             complement(<1..1346)
                     /locus_tag="PKZ74_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM92892.1"
                     /translation="MKEHIAHMVRDRATRFGSDEVFRFMDKKDESYKSYSWESFASDS
                     EKIACSLISMGFGHESRIGIFSDNRLEWTLADIGILAVRGVVVPFFGTASVKQVQYIV
                     DETEMELMFAGNQEQLDKAIWLLDHSKTLKTVVYFPGATACADKRCLAWEQFLQLGEG
                     PVYNHEFQLAFDNAQPYDLATILYTSGTTGEPKGVMLGHDNFMECFLIHDKRLDITRK
                     DVSLCFLPLSHIFERTWTFYLMHCGAVNVFLENPREVIKVLPLVNPTVMCTVPRFFEK
                     TYDGIQVEKEKWPKIKQNIFNWAVATGLRCNEHKKSSSKIPVVLNFKLKLADLLVLKK
                     LRKVFGNKMRQMPCAGAAIREDLLRFFHAAGLFVNYGYGATETTATVSCFKKEEYSFQ
                     TCGTAMPGLDIKFTEEGEIMVKGPTVFRGYYRKPAETEMVLRDGWYMTGDKGHMTK"
     gene            1502..4342
                     /locus_tag="PKZ74_08785"
     CDS             1502..4342
                     /locus_tag="PKZ74_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPM92893.1"
                     /translation="MKKPVKIITAVILILVLAIILLPVIYKGKIKGILLDEAEKNLDA
                     RLDIESVNISLIKSFPSFTIGLKGLTIVGKGAFETDTLLQVESVTITTGFMDLLNGSP
                     YSIRKILIDRADVRMKVLADGRANWDIAPASEEPVMEEQTSTGGSFRLLLKDLSINNS
                     RFIYDDIPNLTYAGLEDFNFSMNGDLGDERTVLGLKAGIGSALVRYDGVEYLKNAKIT
                     WDAGIDADLKNSIYTFADNKLYINDFPLIFNGSVGLPDNGYDLDLSFSTPSGGFKALL
                     SLVPAVYARDFESVKTDGTVSFNGFVKGVYSEDGYPAFKVDLSVGNAWFRYPGLPAEV
                     NKININGTVENPGGDLDNTVIDIRELSLNLADNPVMARLYLKNPMSDPFIDMEVNIDL
                     ALADIGKLYPLSPGEQVTGQIMADVAVKGNLSDFEKLNYTKIEASGKLNSSGIRYSTA
                     YLAQPLQIDRISLSVTPAFLDLADLKIRAGNSDFGLTGKVTNYLGYYLKNETLGGIFV
                     LESKRIDANELLTFAGTEEEPSSSDTAAVSAFIVPPGLDLTLKISAGSVAWLDYDIRN
                     LNGTVRVKDQKLLLDGLTMNGMGGILRMDGSYATTDPVNPTIDFSLGLKDISIRETFK
                     QIGIVEKFAPVAEKIAGDISGNFKLSGLLDETMMPKMESLLGAGDLTTTAIQVNNVNT
                     LNQLSNSLKMDQLRELKINGARIIVEFINGAMNVKPFDFKALGIDMNLGGRTSLDQTI
                     GYELKMKIPRTMMGGEANKVVDDLLATAGRAGADLKPGDQINVDAMIDGTITDPKVKL
                     NLAGMGKNMVENLKEQVKEEVEKKVEEVKDMAKEEAAKILAEADKQAQAILDLASRQS
                     EELLRNAQKLADETKKTANTQADNIVKEAKGKGFIAEKAAEKSAEEVRKQGDKKAQGI
                     MDEARKQSGSVLAKAKQEADKVKADAQKRIGQ"
     gene            4344..4949
                     /locus_tag="PKZ74_08790"
     CDS             4344..4949
                     /locus_tag="PKZ74_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92894.1"
                     /translation="MIVRILRLLPVLIVLLQAGCSQQNDPVRQSVLRQLDRYPQSTLQ
                     DIYKSFFQDEFGPGHMLEDTAAARKYLAYELANMTSGGNYTAEPCGAGLNFYRVPLDL
                     VKDEKISFNEYLTAFLESGSSFRIPATGEWKAKWGMIVLEIESMNLEINNFESDKKDL
                     SKMLDEGETTVHHSESYSLAYDPHYRIMSRVQCERLVGSVR"
     gene            5179..5976
                     /locus_tag="PKZ74_08795"
     CDS             5179..5976
                     /locus_tag="PKZ74_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPM92895.1"
                     /translation="MNSLVFDIRRFTIHDGPGIRTTVFFKGCPLHCQWCHNPESREME
                     IEKVNMKRVFDGRDFHCEETIGKWYSEKEVMHEIEKDMVFYRESGGGVTFSGGEPLMQ
                     ADALAQLLHLCKERELHTVVDTSGYAEPAAIQKVMPLTDLWLFDLKLMDDLKHVEYTG
                     VSNDLALKNLEMLSSRGNKVIIRFPLVPGMTDGIENLEGIALVMKKNKLNRIDILPYH
                     SIAREKYRRLGQSYLLENLVEPDKNRIDEVRSFFNRLGLETGPDGIS"
     gene            6059..>8267
                     /locus_tag="PKZ74_08800"
     CDS             6059..>8267
                     /locus_tag="PKZ74_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPM92896.1"
                     /translation="MNERIKRLREATLNATNTLSEERAVLLTEFYRSDMAKKVSVPVQ
                     RALAFKYILENKKIWINEDELIVGERGPGPKAVPTYPEICLHSLEDLEILHSRPKVSY
                     KSENAMQEAYREKIIPFWKGRTQRDRIFAAMTPEWLAAYEAGIFTEFQEQRAPGHTVL
                     GDKIFLTGMLDLIGQIDKLLEESSRFQVPGSKSEALKAMKIAAEAIIIYANRYAEALV
                     QSSKFQVPGLKDEERKEELLAMARICRRVPAHAPQTFHEALQYYWFVHIGVVTELNPW
                     DSFNPGRLDQHLFPFYEKGLADGTLDRERARELLQSFWVKFNNHPAPPKIGVTAKESS
                     TYTDFALINVGGLKADGSNAVNELSYLILDVVEEMRILQPSSMVQLSVENPDAFIERA
                     MHIVKTGFGQPSIFNTEAIVAELVRQGKSLEDARNGGASGCVESGAFGKEAYWLTGYF
                     NLPKVLEITLHGGLDPRTGKQVGMASRGMASLESAFQAQLNYFIDIKIKGNLTIEQLF
                     AEHMPAPFLSLLIDDCIANGLDYNAGGARYNTSYIQGVGLGTVTDMLAALKYWVYDDA
                     AAGRYDGKTLSMEVLMEALRADFVGFEELRYKLVYETPKYGNDDDFADELAVKVFEMF
                     FSAIDGKPNARGGKHHIMMLPTTSHVYFGSVTGATPDGRKAWEPLSEGISPVQGADIN
                     GPTAVMKSAARIDHLRTGGTLLNQKFTPQILESAKGITAAAQLVRSYFRMNGHH"
BASE COUNT         2255 a         1856 c         2125 g         2031 t
ORIGIN      
        1 tcttttgtca tatgaccttt gtctccggtc atataccagc catctcgcaa taccatttcg
       61 gtctcggccg gtttccgata atacccgcgg aataccgtcg gccctttgac catgatttcg
      121 ccttcttcgg tgaattttat atccaggccg ggcattgcgg ttccgcaggt ctgaaaacta
      181 tattcctcct ttttaaaaca ggataccgtt gcggtagttt ctgttgcccc gtaaccataa
      241 ttgacaaata gtcctgcagc atgaaagaac ctgaggagat cttcacggat ggctgcgccg
      301 gcacacggca tctggcgcat tttgtttccg aataccttcc ggagtttctt cagtacaagc
      361 aggtcggcaa gttttaattt aaaattcaga accaccggaa tttttgatga agattttttg
      421 tgctcgttgc accgcagccc cgtcgctaca gcccagttaa aaatgttttg cttgattttg
      481 ggccattttt ctttttcaac ctgtatcccg tcatacgttt tttcgaaaaa ccttggcacg
      541 gtgcacatga ccgtaggatt gaccaacggc agaactttga tgacttccct ggggttttca
      601 agaaaaacat tcactgcacc gcaatgcatc aggtaaaagg tccaggtccg ttcaaatata
      661 tggcttaagg gcaggaaaca aagtgatacg tccttgcggg taatatccaa tcgtttatcg
      721 tgaataagga agcactccat gaaattatca tgccccagca tgacaccctt gggctcgccg
      781 gtagtgcccg atgtatataa aatcgtggcc aggtcatagg gctgtgcgtt atcaaaagca
      841 agctgaaatt catggttata caccgggcct tcacccagct gtaagaattg ttcccaggca
      901 aggcatcttt tatccgcaca cgctgtcgcg ccgggaaaat aaacaaccgt tttaagcgtt
      961 ttcgaatgat cgagcagcca gatagcttta tccagttgtt cctgattacc tgcaaacatc
     1021 aactccatct ccgtttcatc gacaatatat tgtacctgtt tgaccgaagc agtgccgaaa
     1081 aacggtacca ctacacccct gacagcgagg atgccaatat cggccaatgt ccactccaga
     1141 cggttgtcac tgaaaatacc gatgcgggat tcgtgcccga aacccatgga aatcaatgag
     1201 caggcgatct tctccgaatc actggcaaac gattcccatg aataactttt atacgattca
     1261 tctttcttat ccatgaaccg gaaaacctca tcggaaccga accgggtggc acggtcacga
     1321 accatatggg caatgtgctc tttcattttg tctggaaata aattattgaa taacaggcgc
     1381 aaatgtgaga ttttttatta aatattcaat atttaacatg taaatggtta aaaaaacttg
     1441 tcttatttat gattaaattt gctttttctg taatggaaat ccttacataa tcttagtaaa
     1501 catgaagaaa ccggttaaaa ttataacagc cgtcattctg atcctggtat tggcgataat
     1561 tctgctaccc gttatctata aagggaaaat caaaggaatt ttactcgatg aggccgaaaa
     1621 gaaccttgac gcccgcctgg atattgaaag tgtcaatatt tccctgataa agagctttcc
     1681 ttcatttacc ataggattga aaggactgac catcgtcgga aaaggggcct ttgaaaccga
     1741 taccctgctt caggtcgaat cggttaccat cacaaccggt tttatggacc tgctgaacgg
     1801 aagcccttac agtatccgga aaattctgat cgacagggct gatgtgcgca tgaaagtcct
     1861 ggcagatggc cgcgccaact gggatattgc cccagcttcc gaagagccgg taatggagga
     1921 gcaaaccagt acaggcggaa gcttcaggct tttactgaaa gatttgagca taaacaattc
     1981 ccggtttatt tatgatgata ttccaaacct gacctatgcc ggcctggagg attttaattt
     2041 ttccatgaac ggcgatctgg gagatgaaag gaccgtattg ggtttgaagg ctggaattgg
     2101 ctcggcctta gtccgttacg acggagtgga gtatctgaaa aacgcgaaga tcacctggga
     2161 tgccggtatc gatgccgatt tgaagaacag tatttatacg tttgccgata ataagttgta
     2221 tatcaatgat tttccgttga tcttcaacgg ttcggtcggg ctgcccgaca atggctatga
     2281 cctggacctt tctttctcga caccgtccgg cggttttaaa gcgctgcttt cgctggtacc
     2341 agccgtttat gccagggatt ttgaatctgt aaaaaccgat ggaacagtca gtttcaatgg
     2401 ttttgtcaaa ggtgtatatt cagaggatgg ctatcctgct ttcaaagtcg acctcagtgt
     2461 gggtaacgcc tggttcaggt atcccggtct tccggcggag gtcaataaaa ttaatatcaa
     2521 cgggactgtc gaaaacccgg gcggcgatct tgacaatacc gttatcgata tccgcgaact
     2581 gagccttaac cttgccgata atcctgtcat ggcaaggctt tatcttaaaa atcccatgtc
     2641 ggatcctttc atcgatatgg aggtcaatat tgacctggca ctggcggata tcggcaaact
     2701 ctacccgttg tcgcccggag aacaggtgac cggccagatc atggctgatg tggctgtgaa
     2761 aggtaatctg tctgattttg aaaagcttaa ttacacaaag attgaagcat cgggtaaact
     2821 gaattcctct ggtatcagat attccactgc ttacctggcc caaccgcttc agatcgacag
     2881 gatctcccta tcagtcacac cggcattcct tgatctggca gaccttaaaa ttcgtgccgg
     2941 gaacagcgat ttcgggttga ccggcaaggt caccaactac ctgggttatt atcttaaaaa
     3001 tgaaacgctg ggaggaatct tcgtcctgga atcgaaacgg atagatgcca atgagttatt
     3061 aacatttgca ggaactgagg aggagccctc ctcatcggat acggctgccg tttctgcttt
     3121 tatcgttccg cccggtctcg atctgacact caaaatttca gccgggtcgg ttgcctggct
     3181 ggattacgat attcgcaacc tgaacggaac agtcagggtt aaagaccaaa agcttttgct
     3241 tgatggcctt accatgaatg gaatgggagg gatactccgg atggatggaa gctatgccac
     3301 aaccgatccg gtcaatccaa ccattgattt cagcctggga ctgaaagata taagtatccg
     3361 cgaaacgttt aaacaaatcg ggattgtcga aaagtttgcc ccggttgccg aaaagattgc
     3421 cggcgatatt tcgggaaact tcaagctttc aggtttgctg gatgaaacga tgatgcctaa
     3481 gatggaatcg ctcctgggag ccggcgatct cactacaacc gccatacagg tcaataatgt
     3541 gaatacactt aaccagttgt cgaattcatt gaagatggac cagcttcggg aactgaagat
     3601 caatggtgcc cggatcattg tggaattcat caacggcgcg atgaatgtca agccatttga
     3661 cttcaaagca ctgggcatcg atatgaacct gggaggccgg acgtcgctcg atcagaccat
     3721 tggctacgaa ttgaaaatga agattcccag gacaatgatg ggcggcgagg caaataaagt
     3781 ggtcgacgac ttgctggcga ctgccggacg tgccggagct gacctgaaac ccggcgacca
     3841 gataaatgtc gatgctatga tcgatgggac gattaccgac ccgaaggtaa aactcaacct
     3901 ggcagggatg ggcaagaaca tggttgaaaa cttgaaagaa caggtgaagg aggaggtcga
     3961 aaagaaagtg gaggaggtga aagacatggc aaaagaggag gctgcgaaga tcctggcgga
     4021 agcggataag caggctcagg caattctcga cctcgcctcc aggcaatcgg aagagctgct
     4081 gcgaaatgcg caaaaactgg ctgacgaaac aaagaaaact gctaatacac aggccgacaa
     4141 tatcgtgaaa gaagccaaag ggaaaggatt tatcgcggaa aaggcggccg aaaagtcagc
     4201 agaagaggtg cggaaacagg gcgataaaaa ggctcaggga attatggacg aagcacggaa
     4261 acaatccgga tctgtgctgg ccaaagcgaa acaggaagca gataaagtca aggcagatgc
     4321 gcaaaaaagg attggtcaat aaaatgattg tacgcatttt acgcctgcta cctgtactta
     4381 tagttctact ccaggctggt tgcagtcagc agaatgaccc ggtcaggcaa tcggttttac
     4441 gacagttgga tcggtatcct caatccaccc tccaggatat ttacaagagt tttttccagg
     4501 atgaattcgg gccggggcat atgctggaag atactgccgc ggcacgaaag tacctggctt
     4561 acgagctggc caacatgacc tccgggggca attacacggc tgaaccctgt ggcgccgggt
     4621 tgaattttta cagggttccg cttgacctgg tgaaagacga aaaaatttca tttaatgaat
     4681 atctgactgc ctttctggaa agtggttcct ctttcaggat tccggcaacc ggtgaatgga
     4741 aagcaaagtg ggggatgatt gtattagaaa tagaatcaat gaatctggaa atcaataatt
     4801 ttgagtcaga taagaaagac ttgtcgaaga tgttggatga aggagaaact acagttcatc
     4861 atagcgaatc ttactcgctc gcatatgatc cgcactatcg tatcatgagc agagtacagt
     4921 gcgaaaggtt ggtaggatct gttcgttgaa gaagtgcagg gctaaagccc aaaacataga
     4981 accgcttgca attcgagagt aactttttat ctttgtcaaa aaattcaccc atgctattgc
     5041 cgcattatca tctcttccaa atgtacactc gccgcggccg gggattgttt tgttgtcgta
     5101 tgacgtgaag tcggtcggag gtcgtaagtt tttcgagacc atatacatgc agctaattta
     5161 agtataaaat tttaaatttt gaactccctc gttttcgata ttcgtcggtt tacaattcat
     5221 gatggccccg gaatccggac caccgttttt ttcaagggct gtccgctaca ctgccagtgg
     5281 tgccataacc cggaaagccg tgaaatggag atagagaaag tgaacatgaa aagagtattc
     5341 gacggaaggg attttcattg tgaagagacc atcgggaagt ggtattctga aaaagaagtg
     5401 atgcacgaaa ttgagaagga tatggtgttt taccgggaat cgggcggggg agtcactttt
     5461 tcgggtggcg agccgttgat gcaggccgac gcgcttgcac aattattgca tctttgtaag
     5521 gaaagagaac tgcatacggt cgtggatacc tccgggtatg ccgaacctgc tgcgatacaa
     5581 aaagttatgc cgctgaccga tttatggctg ttcgacctga aactgatgga tgacctgaaa
     5641 catgttgaat atacaggagt ctccaatgat cttgcattga aaaacctgga aatgctgtca
     5701 tcccggggca ataaggtcat tatccggttc ccgttggtcc cgggaatgac ggatggaata
     5761 gaaaatcttg aaggaattgc tttggtgatg aaaaaaaata agctgaaccg gatagacatt
     5821 ttgccatacc attccattgc cagggaaaag tacaggcgcc ttgggcaatc ctacctgctg
     5881 gaaaacctag tggaacccga taaaaataga atagatgagg tcaggtcatt ttttaaccga
     5941 ttgggattgg aaaccggacc ggatggaatt tcataaattt agtgtaactt ggtgatttcg
     6001 tgcttttgtg gcatgtaatg gaatacctgt taaaatcttc gataactgcc agaataaaat
     6061 gaatgaaaga ataaaaaggc tgagggaagc gactttaaat gcgacgaaca cgctttcaga
     6121 ggagagggcg gtgctgttaa ctgagtttta ccggtcggat atggcaaaga aagtttccgt
     6181 tccggttcag agagcactgg cattcaaata tatactggag aataaaaaga tctggattaa
     6241 cgaagacgag ctgatcgttg gggagcgggg gcctgggccc aaggcggttc cgacttatcc
     6301 cgaaatctgc ctgcactcac tcgaagatct cgaaatcctt cattcccggc cgaaggtctc
     6361 ctacaaatcg gaaaatgcca tgcaggaggc gtaccgcgaa aaaatcatcc cattctggaa
     6421 agggaggaca cagcgtgacc ggatctttgc cgccatgacc cccgagtggc tggcagctta
     6481 tgaagccggc atttttaccg aattccagga acagcgggcg ccgggacata cggtgctggg
     6541 ggataagatt ttcctgaccg ggatgctgga tttgatcggg cagatcgata agctcctgga
     6601 ggagagttcc aggttccagg ttccaggttc aaagtctgaa gccctgaagg cgatgaagat
     6661 agcggcggaa gcgatcatca tatacgcaaa ccggtatgct gaggcgttgg ttcaaagttc
     6721 caagttccag gttccgggtt tgaaagatga agagagaaag gaggagctgc ttgcgatggc
     6781 ccggatctgc cgccgggttc ctgcgcacgc accgcagaca ttccacgaag cactgcagta
     6841 ttactggttc gtccatatcg gcgtcgttac ggagctcaat ccctgggatt cgttcaatcc
     6901 gggccgtctc gaccagcact tgttcccctt ctatgagaaa ggattggccg atggcaccct
     6961 tgaccgcgaa cgggccaggg agttgctgca gtcattctgg gtaaaattca ataatcatcc
     7021 ggccccgcca aagattggcg ttacggctaa agagagcagc acttataccg atttcgccct
     7081 gatcaatgtc ggcggattga aagccgatgg gagcaatgcc gttaatgaac tcagctacct
     7141 gatcctggat gtggtggaag agatgcggat tctgcaacca agctccatgg tccagctaag
     7201 tgtggaaaac ccggatgcgt tcattgaaag ggccatgcat atcgttaaaa ccggtttcgg
     7261 ccagcccagt atttttaata cggaagcaat cgtagccgaa ctggtccgtc agggaaaaag
     7321 tcttgaagat gcgcgcaacg gaggcgccag cggttgtgtg gagagcggcg cttttggcaa
     7381 agaggcttac tggctgacag gctatttcaa cctgcctaaa gtgctggaga tcacgctaca
     7441 cggcggtctc gatccgagaa ccggtaagca ggtcgggatg gcgtcccggg ggatggcgtc
     7501 ccttgagtct gcttttcaag cccagctaaa ctatttcatc gatataaaaa tcaagggcaa
     7561 cctgacaata gaacaactct ttgccgaaca tatgcccgcg ccattcctct ccctgctgat
     7621 cgacgattgt atcgccaacg gcctggatta caatgccggc ggcgcccggt acaatacgtc
     7681 ttatatccag ggcgtgggac tgggaacggt aacggatatg ctggctgcgc tgaagtattg
     7741 ggtttacgat gatgcggcag caggacggta tgacggtaag actttaagta tggaggtgct
     7801 gatggaggct cttcgtgctg actttgtggg ttttgaagag cttcgttaca aactggtcta
     7861 cgaaacgccg aaatacggca atgacgatga ttttgccgat gaactggcag tgaaagtctt
     7921 tgagatgttt ttcagcgcta tcgacgggaa gccgaatgcc cgcggtggaa agcatcatat
     7981 catgatgctg ccgaccacaa gtcacgtgta ctttggcagc gtcaccggcg ccactcccga
     8041 tggccggaaa gcctgggagc cgctgtctga aggcatttca cccgtgcagg gagccgatat
     8101 caacggacca acggcggtca tgaaatcggc agcaaggatt gatcacctca ggaccggagg
     8161 caccctgctc aaccagaaat ttacccccca gatcctggaa tcggccaaag ggattactgc
     8221 agcggctcag cttgtgcgca gttatttccg gatgaatggc catcatg
//
LOCUS       DAOPNH010000149         8202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9700_length_8202_cov_7.242175, whole genome shotgun sequence.
ACCESSION   DAOPNH010000149 DAOPNH010000000
VERSION     DAOPNH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9700_length_8202_cov_7.242175"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1299)
                     /locus_tag="PKZ74_08805"
     CDS             complement(16..1299)
                     /locus_tag="PKZ74_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPM92897.1"
                     /translation="MRYIRTLFLISLLMMGAAAVRAQENSLNPTVHGNFQLDAQSYMT
                     DEALGITDSVLQGKSMRMNGYGDIRFSMWRFNAGIRYEAYMPPLAGYDQQYQGQGIPY
                     WFVDYGDEKLQVTAGHFYEQFGMGMILRSYQQWDLGYDNSINGLRVKYSPVAGLNFKA
                     LIGTQRYYWERFEDDNRGVVRGFDGELMLNDAFKAMKEKKTRIILGGSFVSKYEKMRT
                     KSLVVDTVKYEYQLPENVAAAAGRLTISNGGFNFYSEYVYKFNNPSAYNNFIYKPGEA
                     FLASLSYSQKGLGIFVAAKRIDNMSYKSKMTELNNVLDINYIPPLTKQFVYSLENVYP
                     YATQLNGETAFQGQVVYTVPKGTKLGGKYGTKFELNFSRTHGLDKEPVEEGIPVDSTG
                     TDGYKAPWLSLGDLYHQSISATMEKKMGKKQINLP"
     assembly_gap    1386..1485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1515..1976)
                     /locus_tag="PKZ74_08810"
     CDS             complement(1515..1976)
                     /locus_tag="PKZ74_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM92898.1"
                     /translation="MAQEGILEKTIPAVDIKTLQGETVNTSSIDNQGKPMIISFFALW
                     CKPCMRELTAISDVYADWQEEIGVKLVAISIDDSRSVANVLPTVNGNGWEYEFYSDVN
                     GDFKRAMGVNMIPHVFVVDGNKKVVYQHTSYAEGGEHELYEIVKKVAEGSK"
     gene            complement(2040..3017)
                     /locus_tag="PKZ74_08815"
     CDS             complement(2040..3017)
                     /locus_tag="PKZ74_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPM92899.1"
                     /translation="MNELYPLKFKPVFDDRIWGGEKIRTHLGLDFAPLNRCAEAWILS
                     GYEGKQTVVSNGFLEGNEINELIEIYMDDLVGEKVYENTGETFPLLVKFIDSRDWLSV
                     QVHPDDELAQKSGMPNGKTEMWYVIDAEENAQLIAGFNRKITQKQFLEHLHNKTLPEI
                     LNYETVRKDDAIFMPAGRIHSLGPGVLLAEIQQTSDATYRLFDWHRKDSQGKERELHT
                     ELALAAIDFDAFSDNKTNYKPRLNETVDLVSCPYFTTNLIELAQPLRKNYEEIDSFVV
                     YVCLEGAMRIKCGDSPTTVAKGEVVLIPAMFDVVEIYPDGNVKFLEVYI"
     gene            3208..3798
                     /locus_tag="PKZ74_08820"
     CDS             3208..3798
                     /locus_tag="PKZ74_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPM92900.1"
                     /translation="MDSICVFCGSSMGKKPEYKKAAEELGRLLLERNIRLVYGGANVG
                     LMQILADTVLKGGGEVIGIMPQMLIEKEVAHANLTKMITVGSMSERKSRMVELSDAFI
                     AMPGGFGTLDELAEVITYNQLRLTDKPIGILNTAGYFDPLVAFFDHITEEGFLRYEHR
                     QNLIVDDNVGVLLRKMIEFQPVAMDKWIEDIKKESL"
     gene            3841..4863
                     /gene="amrS"
                     /locus_tag="PKZ74_08825"
     CDS             3841..4863
                     /gene="amrS"
                     /locus_tag="PKZ74_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPM92901.1"
                     /translation="MSVHPASYSVKLQENKVKCTLCPHLCVIADGKTGICRVRKNDGG
                     TLISEVYGKACCLRFDPIEKKPLYHFYPGSSILSVGSVGCNLRCKFCQNWEISQTCAE
                     DYPYLKPASVDEIIRMAGERHDNAGIAFTYNEPVIWFEFMVDIARKSKEKGLRNVMVS
                     NGYINAAPLSELLGCIDAFNIDLKAFTDDFYRRVTGGRLQPVRDTLSMIRKHNRHLEL
                     THLVVTGLNDDDDQFEEMVRWIGGELGPETPLHLSRYFPNFELENKATPLPKLLHLYE
                     MATQYLRYVYLGNVAADEGRITVCPDCGASLIERIGYSTYKKGLDAKGRCRKCSRPVI
                     NYIETA"
     gene            complement(4841..6484)
                     /locus_tag="PKZ74_08830"
     CDS             complement(4841..6484)
                     /locus_tag="PKZ74_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM92902.1"
                     /translation="MLKENFSRYLADSITRNWDYRALTDYQGESFYYHDVAGHIVRLH
                     QFFREAGIRPGDKIGLIGKNSARWGILYITTVTYGAVIVPILPDFKPEDLQQIVDHSD
                     AVILFAEEGIFEKMDRANMPKLKCVVSVTSLEFLAGDEEIRAVHSRNEFHKIVPGFYK
                     DEDKPAFPEIPGETLAVISYTSGTTGFTKGVMLTHNNLAANMRYAQQNMPLNPGDRIV
                     SFLPLAHTFGAAFEFLFPFTLGCDITILTKTPSPQVIIKAFQEVKPSLILSVPLVIEK
                     IYKKQVLPAIRKPHMKLLLMLPGLKNIILKKIREKLVAVFGGNFRELVIGGAAFNAEA
                     EEFFRQMKFPFTVGYGMTECGPLISYVGWKDSRPGSAGKAVDTLEVKIDSPDPANKSG
                     EIIVRGDNVMLGYYKNDEATQEVLGADGWLYTGDLGRMDKDGHIYIRGRIKSVLIGSS
                     GKNIYPEEIEAQFNNKPFIAESLVVQRGEKLIVLISPDFEEMEKAGQKEENLAEIYNH
                     YLKTVNHHLPGFMQVSKYEIHPKEFQKTPKRSIKRFLYS"
     gene            6575..7147
                     /locus_tag="PKZ74_08835"
     CDS             6575..7147
                     /locus_tag="PKZ74_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM92903.1"
                     /translation="MIIIGITGTLGAGKGTIVDYLVNQRGFLHFSVRAFISEEIVRRG
                     MKVNRDSMVVVANDLRRNNSPSYITDCLYEQAMEANRNSVIESIRTPGEVYSLRKKGR
                     FLLISVDADPKIRYDRISRRKSETDHIDYETFLQNERREMTSDDPNAQNLAKCIEMAD
                     LKFFNNGTIEQLNRQVEAAMGNLEIGQFGN"
     gene            7159..7653
                     /locus_tag="PKZ74_08840"
     CDS             7159..7653
                     /locus_tag="PKZ74_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPM92904.1"
                     /translation="MSNAHSRPSWDEYFLDLAKAAARRATCDRGRSGCVIVRDKQVLV
                     TGYVGSPMGLPHCDDVGHLMKKVVHDDGTVTQHCVRTVHAEQNAICQAARRGIALDGG
                     TLYCRMTPCRTCAMLIINCGIKRVVCQNRYHAGNESEAMFLEAGVKLDYFSDEVVEYE
                     NQKG"
     gene            7775..>8202
                     /locus_tag="PKZ74_08845"
     CDS             7775..>8202
                     /locus_tag="PKZ74_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPM92905.1"
                     /translation="MKTIMTSFACLAIVTIMVSCGGTSEQKPAAPGTVPAAKPASDDP
                     KANKGIGPVKELTLGELDPAMAAEGEEIFKKMCSACHKPAEKFIGPAPKGILDRRSPE
                     WIMNMILNPEEMVKYDEIAKKLLIEANLAPMANQHLTQEQA"
BASE COUNT         1994 a         2000 c         2052 g         2056 t
ORIGIN      
        1 atgacggtct tatttttagg gcaaatttat ttgctttttt cccatcttct tctccatcgt
       61 tgccgaaatg ctctggtggt agaggtcgcc gaggctgagc cagggcgcct tgtagccatc
      121 cgttccggtg gaatccacag gtatcccttc ctcaaccggc tctttgtcaa ggccatgcgt
      181 cctcgagaag ttcagctcga acttcgtccc gtacttcccg ccgagctttg tccctttcgg
      241 aacggtgtac acgacctgcc cctggaaggc cgtctccccg ttaagctgtg tggcataagg
      301 gtacacattc tccagcgagt aaacgaactg tttcgtcagt ggcggaatat agttgatatc
      361 cagtacgttg ttgagctcgg tcatcttcga cttgtagctc atgttgtcga tccttttagc
      421 agcgacaaaa atgcccagtc ctttctgtga atacgaaagc gaagccagga aagcttcgcc
      481 gggcttatag atgaaattgt tgtatgccga cggattgttg aacttgtaaa cgtattcgga
      541 atagaaattg aacccgccgt tggagatcgt cagccggccc gctgcggcag ccacattttc
      601 gggcagctgg tattcatact tcaccgtatc gaccaccagc gatttggtcc gcatcttctc
      661 gtatttgctc acaaaacttc caccgaggat tatgcgtgtt ttcttctctt tcatagcttt
      721 gaaggcgtcg ttgagcataa gctcgccgtc gaaaccgcgc accacgcccc ggttgtcgtc
      781 ttcgaaccgc tcccagtagt agcgctgggt accgatgagc gctttgaaat tcagtcccgc
      841 gacggggctg tattttaccc gaaggccgtt gatggaattg tcgtagccca ggtcccactg
      901 ctggtaactg cgcaggatca ttcccatgcc gaactgctcg taaaaatggc cggcggtaac
      961 ctgcagtttc tcatccccgt aatcgacaaa ccaatagggg atgccctgtc cctggtattg
     1021 ttggtcataa ccagccagcg gaggcatgta agcttcatag cggatgccgg cattaaaacg
     1081 ccacatcgaa aagcggatat cgccatagcc gttcatccgc atgctcttgc cctgcagcac
     1141 cgagtcggtg atccccagcg cctcatcggt catgtacgat tgcgcatcaa gctgaaaatt
     1201 cccgtgaacc gtcgggttga gggaattttc ctgcgcccgc accgcggcag cccccatcat
     1261 aagcaggcta attaaaaata gagttctaat atatctcata aattccaaat tccaaaatcc
     1321 aaaatccatc ccgagtactc gggaccaaac aaaaaccaaa tctcaaatct tcaaatcgtc
     1381 aaatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaaa tctccaaatc
     1501 ttcaaatcgc caaatcactt gcttccttcc gccactttct tcacgatctc atacagctcg
     1561 tgctcgccgc cttcggcgta agaggtgtgc tggtaaacga ctttcttgtt gccgtcgacc
     1621 acaaacacat gagggatcat attgacgccc atagcgcgtt tgaaatcgcc gttcacatcg
     1681 ctgtaaaact cgtattccca gccatttccg ttcacagtag gcaatacatt ggctaccgag
     1741 cgggaatcgt cgatcgatat ggcaacaagc ttaactccaa tttcttcctg ccagtcggca
     1801 taaacatcgg agatggcggt gagctcacgc atgcagggtt tgcaccacag ggcgaagaag
     1861 gagatgatca tcggcttgcc ctggttatcg atggaagagg tgttgaccgt ttctccctgc
     1921 agggttttga tgtcgacagc cgggatggtt ttttccagga tgccttcctg ggccatgaga
     1981 tttatccctg agaaaactaa aatgaagaat acgattactt ttttcatatc tgattgaaat
     2041 taaatataaa cctctaaaaa cttcacatta ccatcggggt agatctccac cacgtcaaac
     2101 atcgccggga tcaggaccac ttcacctttg gctaccgtcg tcgggctgtc cccgcatttt
     2161 atcctcatag cgccctccag gcagacatac accacgaacg aatcgatctc ttcgtagttc
     2221 tttctcaaag gttgtgccaa ttcgatcagg tttgtcgtaa aataggggca gcttaccagg
     2281 tcgaccgttt cgttcagcct gggcttgtaa ttcgttttat tgtctgagaa tgcatcaaaa
     2341 tcaatagctg caagggccag ttcggtatgc aactcccttt ccttaccttg cgaatctttc
     2401 ctgtgccagt cgaacagtct gtaggttgcg tcggaggttt gctggatctc ggccagcagc
     2461 acgcccgggc ccaatgagtg gatccggccg gccggcatga agatcgcgtc gtctttccgc
     2521 accgtttcat aattcaatat ctccggaagg gttttattat gaagatgttc aaggaactgt
     2581 ttttgagtga ttttccggtt aaagcccgcg ataagttgtg cattttcttc ggcatcgatc
     2641 acgtaccaca tctccgtttt accgttcggc atcccacttt tctgtgccag ttcgtcgtcg
     2701 ggatgaacct gcaccgacag ccagtcgcgt gaatcgataa acttgacaag caagggaaat
     2761 gtttcgccgg tattttcgta aaccttttcg ccgaccaggt catccatata gatttcgatc
     2821 agttcattga tctcattgcc ctccaggaac ccattactga caacagtctg ttttccttcg
     2881 taaccggaaa ggatccaggc ctccgcgcac cggttcaaag gggcaaagtc aagacccagg
     2941 tgtgtcctga tcttttcacc tccccagatc cggtcatcga atacaggctt gaatttcaaa
     3001 gggtaaagct cgttcacgga atttttgatg aagcgccaaa aatactgttt tttggattat
     3061 atgttgaaat cgttatgatt agaatgggtt taaattagtt ccggagcctg ccctgattcc
     3121 gccaaaggcg ggcgaaggat tccagagttc caggttccag agttccagtt ttggaaatat
     3181 tctttatttt ggctgaaaat taccggtatg gattccatct gtgttttttg tgggtcgagc
     3241 atgggcaaaa agcctgaata caaaaaggct gcggaggagc tgggcaggct gctgctggag
     3301 cggaacatcc ggctcgtata cgggggagcc aatgtgggat tgatgcagat cctggccgat
     3361 acggtgctga aaggaggggg agaagtcatc ggtattatgc cgcaaatgct gatcgaaaaa
     3421 gaggtcgcac atgcaaatct cacgaaaatg atcaccgtag gaagcatgag tgaacgcaag
     3481 agcaggatgg tagaactatc cgatgccttt attgccatgc ccggcggatt cggcacactg
     3541 gatgaactgg ccgaggtgat cacttacaac cagctccggc tcaccgacaa gccgatcggc
     3601 atcctcaata ctgccggtta ttttgatcct ctggtggctt tcttcgacca tatcacggaa
     3661 gagggctttc tcaggtacga acaccgtcaa aacctgatcg tagatgacaa tgtcggcgtg
     3721 ttgttgagaa agatgattga attccagccg gttgcgatgg ataaatggat cgaggatatc
     3781 aaaaaggaaa gcttatgagc ttctaggctt acaggtacga aaaatgatta tttttacaaa
     3841 atgtccgtgc atcctgcttc atactcggtc aagcttcagg aaaataaggt taagtgcacc
     3901 ttgtgtcccc acctgtgcgt tatagcggat gggaagacag gtatttgcag agtcaggaag
     3961 aacgatggag ggacgcttat ttcggaagtt tacggtaaag cctgctgcct gcgttttgac
     4021 ccgatagaga aaaagccgtt gtatcatttt tacccgggca gttcgatttt atcggtcgga
     4081 tccgtggggt gcaacctgcg ctgcaagttt tgccagaact gggagatctc ccagacctgc
     4141 gctgaagatt atccttatct caagccggct tcggttgacg agatcatccg gatggcgggc
     4201 gaacggcacg acaacgccgg tatagcattc acttacaatg aacctgttat ctggtttgag
     4261 ttcatggtcg acatagcccg gaaatcgaag gagaagggat tgcggaacgt gatggtgtcg
     4321 aacgggtata tcaatgctgc gccgctttct gagctgcttg gctgcatcga cgctttcaat
     4381 atcgacctta aagcctttac cgatgatttt taccgcaggg ttacgggagg aaggctccag
     4441 ccggtcagag acactttgtc gatgatcagg aagcacaacc ggcaccttga gttgacacac
     4501 ctggtggtga caggtctgaa cgacgatgac gaccaattcg aagagatggt gaggtggatc
     4561 ggcggtgagc tgggtcctga aacaccgctg cacctgtcgc gctatttccc gaactttgaa
     4621 ctggaaaaca aggcgacacc gttgcccaag ctgctgcatc tgtatgaaat ggcgactcaa
     4681 tacctccggt atgtttacct gggcaatgta gctgctgatg aagggcggat taccgtttgc
     4741 cccgattgtg gcgccagcct gatcgaacgg atcggctatt caacctataa aaaagggctc
     4801 gatgccaaag ggagatgccg gaagtgcagc aggccggtga ttaactatat agaaaccgct
     4861 tgatactacg ttttggcgtt ttctggaatt ccttcggatg gatctcgtat tttgaaacct
     4921 gcataaatcc cggcaggtgg tggttgacgg ttttcaggta gtggttgtaa atctcagcca
     4981 ggttttcctc tttctgcccc gctttttcca tctcttcaaa atcggggctg atcagcacaa
     5041 ttagcttttc accacgctga acgacgagtg attcggcaat gaacggcttg ttattgaact
     5101 gggcctcgat ctcttcgggg tatatattct ttccgctgga gccgatcagg acgcttttta
     5161 tccggccacg gatatagata tggccatcct tgtccattct ccccaggtcg cctgtataca
     5221 accagccgtc tgcaccgagt acttcctgtg tcgcttcatc gtttttgtaa taccccagca
     5281 tgacattatc gcccctgacg atgatctcgc ccgatttatt ggccggatcg ggcgaatcga
     5341 tcttcacttc aagggtatcc accgccttac ctgccgatcc gggcctggaa tctttccatc
     5401 cgacatagct gatcagaggc ccgcattcgg tcatgccgta gccaacggtg aatgggaact
     5461 tcatctggcg aaaaaattcc tcggcctcgg cattaaaggc cgcacccccg atcaccagtt
     5521 ctcggaaatt tccgccgaaa acggcaacga gcttttcccg gatcttcttc aggataatat
     5581 ttttcaaccc gggcaacatc agcagcaact tcatatgtgg tttgcggatg gcagggagga
     5641 cctgtttttt gtagattttc tcgatgacca gcggcacgga gaggatcagc gagggcttca
     5701 cctcctggaa cgccttgata atgacctgcg gtgacggggt tttggtcagg atggtgatat
     5761 cgcagcccag tgtgaatggg aaaaggaact cgaacgcggc gccgaaggtg tgggccaatg
     5821 gcaggaacga cacgatccgg tcgcccgggt tcagcggcat attctgctgg gcataacgca
     5881 tattcgctgc caggttgttg tgtgtcagca tgaccccctt ggtaaatccg gtcgtgcccg
     5941 acgtataact gatcacagcc agcgtctcac cggggatctc cggaaatgcc ggtttatctt
     6001 catctttata aaaacccggg acgatcttat ggaattcgtt acgcgagtga acagccctga
     6061 tctcttcatc gcccgcaagg aattccagcg aggtgaccga gaccacacat ttgagctttg
     6121 gcatattcgc cctgtccatt ttctcgaaaa taccctcttc ggcaaacagg ataacagcat
     6181 cggaatggtc gacgatctgt tgcagatctt cgggcttgaa atcgggcaga attggcacga
     6241 tcaccgcgcc gtaggtaacg gtggtgatgt acaggatacc ccagcgggcc gagtttttac
     6301 cgatcaggcc aatcttgtcg ccgggtcgta tgcccgcttc cctgaagaat tgatgaagcc
     6361 tgacgatatg tcctgcaaca tcatggtagt aaaagctctc tccctgataa tccgtaagtg
     6421 cgcggtagtc ccagttcctg gtaatgctgt ctgccaggta acgtgaaaag ttttccttca
     6481 gcataatttg gtttaaaaaa aaatcaaagt tagatattat ttgaaaggtt gaaaccacct
     6541 gtttatttta taagtttgtt ttgaaatcaa tagtatgatc atcattggaa taaccggaac
     6601 actgggagca ggaaaaggga cgatcgtcga ttacctggtc aatcaacggg gattccttca
     6661 tttttctgtc agggctttta tttcggaaga aattgtcaga agaggaatga aggtgaaccg
     6721 tgattcgatg gtggtggttg ccaacgacct gcgaaggaac aattcgccgt cttatatcac
     6781 cgattgcctc tatgaacaag cgatggaagc aaacaggaac agtgtgatcg aaagtatccg
     6841 gacgccgggt gaggtttatt cgttaagaaa gaaaggccgg ttcctcctga tttctgttga
     6901 cgccgacccg aagatcaggt atgaccggat cagccggcga aaatcggaaa cggatcatat
     6961 tgattacgaa acctttctgc aaaacgaacg aagagaaatg acttcggacg atccgaatgc
     7021 gcagaacctt gcgaaatgta tcgaaatggc ggatttaaaa ttttttaaca atggaacaat
     7081 tgagcaattg aaccggcaag tggaagctgc aatgggcaat ttggaaattg ggcaatttgg
     7141 aaattagaca atttggaaat gagtaatgca cattcccgtc cttcgtggga cgaatatttc
     7201 ctggatctgg ccaaggcggc ggctagaagg gcgacatgcg accgtgggcg cagcggctgc
     7261 gtcattgtcc gtgataaaca ggtgctggtg accggatatg tcggctcgcc gatgggtttg
     7321 ccacattgcg acgacgtcgg acacctgatg aagaaggtgg tgcatgacga cggcacggtc
     7381 acccagcatt gcgtccgtac cgtccatgcc gagcagaatg ccatttgcca ggcggcccgg
     7441 cgcggcatcg ccctcgacgg cggaacgctc tactgccgga tgactccctg ccgaacctgc
     7501 gccatgctga tcatcaactg cgggatcaag cgggtggttt gccagaaccg gtatcatgcc
     7561 ggcaacgaat cggaagcgat gttccttgaa gccggagtca agcttgatta tttcagcgat
     7621 gaggtggtgg aatatgagaa tcagaagggc tgatattttg attttctgaa tttttgtgat
     7681 caatttcctt ttcttgtaac atatgttaca tattgttcct attgtgacat atatctttac
     7741 cgcaataaat tatcaatcaa attaataaga aataatgaaa acaatcatga cttcttttgc
     7801 ctgtctggca attgttacaa taatggtgtc gtgtggcggt acttcggaac agaaaccggc
     7861 ggcacccggt acggtgccgg cagccaaacc ggcttctgac gacccgaaag ccaacaaagg
     7921 gatcggaccg gtcaaagaac tgacactggg cgaactcgat ccggcaatgg cggcggaagg
     7981 ggaggagata ttcaaaaaaa tgtgctctgc ctgccataaa ccggctgaga aattcatcgg
     8041 cccggcgcca aagggaattc tcgaccgccg ctctccggag tggatcatga acatgatcct
     8101 gaacccggaa gagatggtca agtatgatga aattgccaaa aagctgctca tcgaagcaaa
     8161 cctcgccccg atggccaacc agcatcttac acaggaacaa gc
//
LOCUS       DAOPNH010000150         8147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9795_length_8147_cov_4.787321, whole genome shotgun sequence.
ACCESSION   DAOPNH010000150 DAOPNH010000000
VERSION     DAOPNH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_8147_cov_4.787321"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKZ74_08850"
     CDS             complement(<1..562)
                     /locus_tag="PKZ74_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM92906.1"
                     /translation="MSKKLKFLVFSSIGLLFAGMAYLFFTGMTTKAEENNPSGAEIMF
                     FEEGMNYAVPVPLPDPAFFAGEEVPAGTFYVREQLDREMTVNTYWHSSTLLSMKRSAR
                     WFPVIEPILAKNGIPDDFKYLALAESGLANVVSPAGAAGFWQFLEGTGRECGLIVNKE
                     VDERYHLVKATEAACVYLKAAYQKFGN"
     gene            complement(603..1145)
                     /gene="rfbC"
                     /locus_tag="PKZ74_08855"
     CDS             complement(603..1145)
                     /gene="rfbC"
                     /locus_tag="PKZ74_08855"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPM92907.1"
                     /translation="MKIHKTEIEDLLVIHPDVFPDERGYFFESYQKEKFREAGLNWDF
                     VQDNESMSMKGVLRGLHFQLPPFAQGKLVRVVRGAVLDVAVDLRKNSATYGQWASFRI
                     SAENKWMAFIPAGFAHGFLVLEDHTIFQYKCTNYYNKESESGIIWNDPELAIDWGIEH
                     PLVSEKDLQGLRFKDFVSPF"
     gene            complement(1145..2536)
                     /gene="lpdA"
                     /locus_tag="PKZ74_08860"
     CDS             complement(1145..2536)
                     /gene="lpdA"
                     /locus_tag="PKZ74_08860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPM92908.1"
                     /translation="MNYDLIVIGSGPGGYVAAIRASQLGLKTAVVERESIGGICLNWG
                     CIPTKALLKSAQVFRYAKHAADYGVTIQGDVTADFAGMIKRSRDVANGMSNGVQYLFK
                     KNKIDLIKGEGKVAPGKKVFVVDESGTKAEYTANHIILATGTRSRELPNLKQDGVKVI
                     GYRQAMNLAVQPKSMVVIGSGAIGSEFAYFYNTIGTEVTLIELLPNIVPLEDEEVSKQ
                     LERSFKKAGIKVMTDAAVESVDTGGPSCKVKIKTKKGEEFIECDVVLSAVGVKSNIEN
                     IGLEEVGIQVEKDKIKVDEYYRTNVEGFYAIGDIIPGPALAHVASHEGITCVEKIAGL
                     HVEPIDYNNIPGCTYTSPEVASVGYTEKQAREAGYEIKVGKFPYTASGKASAAGNKDG
                     FIKVIFDAKYGEWLGAHFIGDNVTEMIAEVVVARKLETTGHEILKSVHPHPTMSEAVM
                     EAVAAAYGEVIHI"
     gene            2718..4181
                     /locus_tag="PKZ74_08865"
     CDS             2718..4181
                     /locus_tag="PKZ74_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM92909.1"
                     /translation="MSTGKQYYIDAAGRATSWHQENIERMRRCRFDTIAVHGIYSMQE
                     ALDFNQGSIIEPVYMSTSQAYRDADEMEAALGYEIPTWCYSRIANPSMYYLEGVLALL
                     DGYGAKDATSCVATSSGMAAIMAAADPLLAVDPDHPSKPINFVATCQVYGGTFQQFAI
                     RKHQERNIEWRKVVHSSDINEWASLIDENTRFLYGEMPSNPGQAFFDLEKVVELAHSH
                     NIPMIVDATVATPALMRPIQWGADIVVQSVTKTLATSGFAIAGAVIAKKKIVTNIKND
                     ALRDDYCMYLKALPNRDNGPNISPMNAIMALNDIRTLRMRVDQCSRTTLKVAQFLAQH
                     KHIEQVDYLGLESHPLHPLASKYLWLVDAETDEIYGKPVNRYGHLMSFRVKGGGQAAR
                     DVFDGMQRIWRATDLGRIKSVATIPAISTHSQQGEEGRKIADIPPNLVRLCVGAEHPD
                     DIIADLEQALAVLDGKKVSVTSPEYSAGGASSARIRS"
     gene            complement(4176..4919)
                     /locus_tag="PKZ74_08870"
     CDS             complement(4176..4919)
                     /locus_tag="PKZ74_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GW dipeptide domain-containing protein"
                     /protein_id="HPM92910.1"
                     /translation="MIKKISLYSLFIAITAVSCNFGQKPDEYAHQKAKVREVIQTTNY
                     TYLRVEKSKQDQWIAIGKSDIKEGSVIYFEDGLQMVNFESPELGRTFESILFVQQISD
                     KPIKHGDFHGEMPEGMGEMGGMDPAMMGDKPEKPVIDKIEIELEHPAGAIKIGDLYAR
                     RSEYAGKQVTVHGKVIKVNVGIMGKNWLHIQDGTSDGDNFDLTITTIYEPAVGAVVTY
                     SGTLAVKKDFGYGYFYEIIVEDAEPVKAL"
     gene            5083..>5417
                     /locus_tag="PKZ74_08875"
     CDS             5083..>5417
                     /locus_tag="PKZ74_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM92911.1"
                     /translation="MSCRNLLFTVLPVFLFLELNAQVFINEGSNRNYSLIADENGEFP
                     DWVELYNAGNETISLYNYSLSDNIDEPGKWTFPNVDIDPGEYRVVFCSGKDRKPVTGF
                     VNVVHTGPFV"
     assembly_gap    5418..5517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5518..7812
                     /locus_tag="PKZ74_08880"
     CDS             <5518..7812
                     /locus_tag="PKZ74_08880"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM92912.1"
                     /translation="VGNITSLGFDVVSTDPNTTYDYIDISLKLVTVSEITQEFTPVDT
                     NNFLHTNFKLKKNGETVYLFSPGHTLLGSLSIGCGDLDNSLGRQPDAGSETYLFQAGT
                     PGASNNASATFTDYLEPPSFSIPSGFFNQPFSVSITNPNPGQTSVHYTIDGSEPNILS
                     PLYTGQPIEVMYSIVLKAKAFGWGILPSRNTVASYLFGVNHTTPVISVVTDDDNLYGE
                     RGIFDNWWTDWERTAYVEYFDSLQNLIFSQRTGMQIDGGWGGARYQPQHSFRLELDDG
                     VLGDGPVEYPIIPDRPERNKYGQIYLRNGSNQYLVLPYKDACQVTAMGTGTYNYYSAW
                     RPVSVYMNGYYFGLYELREKIDAEYFEVYDDADNDEMDLLSLSAWYGFAFRALEGSVD
                     NFFEDYYYFSLLEPSDTGYWKQADNYFDLKYYTDYIIGESWMANNDWPGNNIKIYRSD
                     KTNYRWRFCLIDLELGMAPNSWTDCYTDHIRYMLDQDPNNPFINIWKRSIENDEYRHY
                     FINRFADVMNTAYLAENIISLENRMFDQVVGEMPKEYARWGDANHIPEQMLTFTENHN
                     IFVSQLAERSTQVRNHIQSNFQLPNQVLVTLGVYPEGAGKINISTIAPDEYPWQGVYF
                     NGVPVRIEALANQGYEFIEWDNNLLLNDTLNPVFQGMLDAESVSFNAIFEEISIGVAS
                     PDNQTLIRVYPNPAMDFIHILNNGNTKGAYYQLINANGQTILEGKLNGDETTLDIRHL
                     TGAVYLLIIVDSDLNRISFRIVKI"
BASE COUNT         2032 a         1946 c         1897 g         2172 t
ORIGIN      
        1 agttgccgaa tttctggtaa gcagccttca aataaacaca ggcagcctcg gtagctttta
       61 ccagatgata cctttcatcc acttctttat tcacaattaa cccgcattcg cggcccgtac
      121 cttccaggaa ttgccagaat ccggcggctc cggcggggga gacaacgttg gccagtccgc
      181 tttcggccag ggccaggtac ttgaaatcat ccgggatccc gttttttgcc aggatcggtt
      241 cgatgaccgg gaaccagcgg gccgaccgct tcatcgaaag cagggtggag gagtgccagt
      301 atgtgttgac ggtcatttcc cggtcgagct gctccctgac ataaaaagtt cccgccggaa
      361 cctcttcgcc cgcgaaaaat gccggatcag gcaaaggtac cggcacagcg taattcatgc
      421 cttcttcaaa gaacattatt tcggctccgg acgggttatt ctcttccgct ttggtcgtca
      481 tccctgtaaa aaacaggtat gccattccgg caaagaggag accaatagag gaaaaaacca
      541 ggaatttcaa ttttttggac atatttgata atgttttgat aagaagttga ggaagatcca
      601 tttcagaaag ggctgacaaa atctttaaaa cgtaaacctt gtaaatcctt ttcggaaaca
      661 aggggatgtt cgatgcccca gtcgatagcc agttccggat cattccagat gatgccgctt
      721 tccgattctt tgttataata attggtgcat ttatactgga agatggtatg atcttccagc
      781 accagaaacc cgtgggcaaa cccggccggg ataaaggcca tccatttgtt ttccgcgctg
      841 atgcggaagg aggcccattg accgtatgtg gctgagtttt tccggaggtc aaccgccaca
      901 tccagcacag cgcccctgac caccctgacc agtttgcctt gtgcaaaggg gggcaactgg
      961 aaatgaaggc cgcgaagcac acctttcatc gacatcgatt cattatcctg cacgaaatcc
     1021 caattgagcc cggcttccct gaatttttcc ttctggtatg attcaaagaa atagcctcgt
     1081 tcatcgggaa aaacatccgg gtggatcacc agaaggtctt cgatttcagt tttgtggatt
     1141 ttcattatat atgaatgact tcgccatatg ctgctgctac cgcttccatc accgcttccg
     1201 acatggtcgg gtgggggtgc accgatttca ggatctcgtg gccggtagtt tcaagtttgc
     1261 gtgccaccac tacttcggcg atcatttccg tgacattgtc gccgatgaaa tgagcgccca
     1321 gccattcgcc gtatttggca tcgaagatca ctttgatgaa cccgtctttg ttgcctgctg
     1381 cgctggcttt tcctgatgcg gtgtagggga acttgcccac ctttatttca tagccggctt
     1441 cacgtgcctg cttttcggtg taacctaccg atgccacctc cgggctggta taggtgcacc
     1501 cggggatgtt attgtagtcg atcggttcga catgcagccc ggcgatcttt tccacgcagg
     1561 tgatcccttc atgcgaggcc acatgagcca gggccggccc cggaataata tcgccaatcg
     1621 catagaaacc ttcgacattg gtccggtagt attcatcgac cttgatcttg tccttttcca
     1681 cctgtatccc gacctcttcc agcccgatgt tttcgatgtt cgattttaca cccacagccg
     1741 atagaacgac atcgcattcg ataaattcct cgcccttttt ggttttgatt ttaaccttgc
     1801 aggatgggcc accggtatca accgattcga cagcagcatc ggtcatcact ttaataccgg
     1861 cctttttaaa gcttctctcc agttgtttcg aaacctcttc atcttccagc gggactatat
     1921 tgggcaacaa ttctatcagg gtgacctctg tgccgatggt gttgtagaaa taggcaaatt
     1981 cactgccaat ggctcccgac ccgataacaa ccattgattt cggctgaacg gcgaggttca
     2041 tcgcctgccg gtagccgatg actttcacgc cgtcctgctt caggttgggc agctcgcgcg
     2101 aacgggttcc ggtagccagg ataatgtgat tggctgtata ttccgccttg gtgccgcttt
     2161 cgtcgacgac aaacactttc tttccgggag cgacctttcc ttcgcctttt atgaggtcga
     2221 ttttattctt tttgaaaaga tactgaacgc cgttgctcat tccgttggcc acgtcgcggc
     2281 ttcttttaat catgcctgca aaatcggctg tcacatcacc ttgaatggtg acgccataat
     2341 cggcggcatg tttggcatac ctgaatacct gtgcgctttt aaggagcgct ttcgtaggga
     2401 tacagcccca gttcaggcat atgccaccga tggattccct ttcgacgaca gcggttttca
     2461 aacccagctg tgatgcgcgg atggcagcca catagcctcc cggcccgctg cctataacga
     2521 taagatcata attcatgaat ttcagaatta aaatttttac aaaggtagta aaagaagaca
     2581 gaagactgaa gacagaagac tgaagtgagc atttgataat ttgaattttt ggcatttgtt
     2641 tggaatttgg aattcggggt ttggaatttc aaattaatat tgtctatttt agcaattcat
     2701 tccaaaccaa ttcaactatg tcaaccggaa aacaatacta cattgatgcc gcgggaagag
     2761 caacgtcatg gcatcaggaa aatattgaac gaatgcggcg ctgccgcttc gatacgatag
     2821 ctgttcacgg aatttactcc atgcaggagg cacttgattt taaccagggg tctattatcg
     2881 aacccgtata tatgtcgact tcacaagcat accgtgacgc cgacgagatg gaagcggcgc
     2941 ttgggtatga gatcccgacg tggtgctatt cccgtattgc caaccccagc atgtattacc
     3001 tggaaggggt cctggcgctg ctcgacgggt acggggcaaa ggatgcaacc tcctgtgtgg
     3061 ccacctcttc ggggatggcg gccatcatgg ctgctgccga tccattgctg gccgtcgatc
     3121 ctgatcatcc ctccaaaccg atcaattttg tggccacctg ccaggtgtat ggcggcactt
     3181 tccagcagtt cgccatccgc aagcaccagg aaagaaacat cgaatggcga aaagtggttc
     3241 attccagtga tataaatgag tgggcttcac tgatcgacga aaatacccgg ttcctatacg
     3301 gtgaaatgcc atccaatccg ggccaggcat tcttcgatct tgagaaagtg gtcgagcttg
     3361 cccattcgca taacatcccg atgatcgtcg atgccaccgt tgccacgccg gctttgatgc
     3421 gcccgatcca gtggggagcc gatatcgtgg tccagtctgt tacgaaaacg ctggctacga
     3481 gcggctttgc catagccggc gctgtaatcg ccaaaaagaa gatcgtaacc aatataaaga
     3541 atgacgctct ccgggatgat tactgcatgt atctcaaagc ccttcccaac agggataacg
     3601 gaccgaatat ctccccgatg aatgccatta tggccttgaa cgatatccgg acattgcgca
     3661 tgcgtgttga ccagtgtagc aggaccacgc tgaaagtggc ccaatttctt gcacagcaca
     3721 aacatatcga acaggtcgat tacctggggc tggaatctca tcctttgcac ccgctggcca
     3781 gcaaatacct ctggctggtc gatgccgaaa ccgatgagat ttatggtaag ccggtcaacc
     3841 ggtacggaca cctgatgtcg tttcgcgtaa aaggcggggg gcaggcagca cgtgatgtgt
     3901 tcgacggcat gcagagaata tggcgggcga cagacctggg caggatcaaa tcggtagcga
     3961 caatcccggc tatttcgacg cactctcagc agggagaaga aggccggaaa atcgccgaca
     4021 tcccgccaaa cctggtccgt ttgtgcgttg gcgccgagca cccggatgat attattgcag
     4081 atctggaaca ggcactggcc gtgctggatg ggaaaaaggt atccgtgact tcgccggaat
     4141 atagtgcagg gggagcgagc tcggcaagga tcagatcata aagcctttac cggctcggca
     4201 tcttctacga taatctcata aaagtatccg taaccaaagt cttttttaac ggccagtgtg
     4261 ccgctatagg tcacgacagc cccgactgcc ggctcgtaga tcgtagtgat ggtcaggtcg
     4321 aaattgtcgc catcggaggt tccatcctgg atatgcaacc agttcttgcc cataatgccg
     4381 acattgactt tgatcacttt tccatgcacg gtgacctgtt ttccggcata ttcgcttctc
     4441 cgggcataca gatcgccaat ctttatcgca ccggccggat gctcaagctc tatctctatt
     4501 ttgtcaatga ccggcttctc gggtttatca cccatcattg ccggatccat tccacccatt
     4561 tcccccatac cttcaggcat ttcgccatga aaatcaccat gctttatcgg tttgtcgctg
     4621 atttgctgca caaataaaat actctcgaag gtccggccca gttccgggct ctcaaaattg
     4681 accatttgca acccatcttc aaaataaatg accgaaccct cttttatatc cgatttgcca
     4741 atggcaatcc actgatcctg tttgcttttc tcaaccctga gatacgtgta gttggtcgtt
     4801 tggatcactt ccctgacttt agctttctgg tgtgcatatt cgtcgggctt ttgcccgaaa
     4861 ttgcatgaaa cggctgtaat agcgatgaac agcgaatata gactgatctt ttttatcatg
     4921 atgaatgaga ttttaggatt gaactattct ttcggatgca aaaatatcaa tacattttga
     4981 atattgtatg tttatttttg agccctgcaa tgccatttaa agcaatacat cataattggt
     5041 cttctgtttc cataaagttt taactttatc aaaaatttct ctatgtcctg ccgaaatctc
     5101 cttttcacag ttctcccggt tttcctgttt cttgaattga acgcccaggt ttttatcaac
     5161 gaaggcagta accgtaatta ttcattaatt gccgatgaaa atggtgaatt ccccgattgg
     5221 gttgaattgt ataatgccgg aaacgagacg attagcctgt acaactattc gttgagtgat
     5281 aatatagatg agcccggtaa atggaccttt cccaatgttg atattgatcc cggagaatat
     5341 cgagttgtgt tttgcagtgg caaagaccgc aaaccggtga caggatttgt gaatgtggta
     5401 catacaggcc cgttcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg
     5521 ggaaatatca ccagcctggg atttgatgtc gtatccaccg atcccaatac aacttacgat
     5581 tatatcgata taagtttgaa attagtgact gtcagtgaga taactcaaga atttactccg
     5641 gtcgatacca ataattttct gcatacgaat tttaaattaa agaagaatgg cgagacggtt
     5701 tacctgtttt ctcccggcca taccttgctg ggttcgcttt cgatcgggtg cggcgatttg
     5761 gataacagcc tgggccgcca gcccgatgcc ggttccgaaa cctatctttt tcaggccggt
     5821 acaccgggag cttccaataa cgcctcggcc acttttaccg attatctgga accgccctca
     5881 ttctcaattc cttccggttt cttcaaccag cccttcagtg tgtctataac caatcccaat
     5941 cccggtcaga catcggttca ttacaccatc gacgggagcg agccgaacat tctatcgcct
     6001 ctgtataccg gtcaaccgat tgaagtaatg tactccatcg ttttgaaagc caaggccttc
     6061 ggatggggca tcctcccgag ccggaatacc gtggcctctt atcttttcgg ggtaaatcat
     6121 acaacccccg tgatcagtgt cgtcacagat gatgataatc tttacggtga aagagggatt
     6181 ttcgataact ggtggaccga ttgggagcgg acagcctatg ttgaatattt tgactccctg
     6241 caaaacctca ttttttcgca acgaacaggg atgcagatcg atggcgggtg gggaggtgcc
     6301 cgataccaac cccagcactc tttccggctg gagctggatg acggggtcct tggcgatggg
     6361 ccggtggaat acccgatcat acccgacagg cctgaacgaa acaaatacgg tcagatctac
     6421 cttcgcaacg ggagtaacca atacctggta ttaccttata aagatgcttg ccaggtaacg
     6481 gctatgggaa caggaaccta taactattat tccgcctgga ggccggtatc agtttatatg
     6541 aacggttatt attttggttt atatgaatta agagaaaaga ttgatgctga atattttgaa
     6601 gtgtacgatg atgccgataa tgacgaaatg gacctgttgt cgctgagcgc ctggtatgga
     6661 tttgctttcc gggcactgga aggctcggtc gataatttct ttgaagatta ctactatttt
     6721 tccctccttg aaccttcgga tacgggatac tggaaacagg cggacaatta tttcgatctg
     6781 aaatattaca ccgattatat catcggtgag tcgtggatgg ccaataacga ctggcccggg
     6841 aataacatca agatttaccg gtcggataag actaattacc ggtggagatt ctgcctgatc
     6901 gaccttgaac tcgggatggc tccgaatagc tggacagatt gctataccga ccatataagg
     6961 tatatgctgg atcaagaccc gaataatccg ttcatcaata tctggaagcg aagtatcgaa
     7021 aacgacgagt atcgccatta tttcatcaac cggtttgccg atgtgatgaa taccgcgtat
     7081 ctcgctgaaa atattatttc gcttgagaac cggatgtttg atcaggtcgt gggcgaaatg
     7141 cctaaagaat atgcgcggtg gggagacgcc aaccatatcc ctgaacagat gctgaccttt
     7201 acggaaaatc acaatatttt cgtttcccag cttgcagagc ggtcaacaca ggtgaggaac
     7261 catatccagt cgaattttca attgcccaac caggtgctcg taaccctggg ggtttatccg
     7321 gaaggggcgg ggaaaattaa tatcagcacg attgcacccg atgaatatcc ctggcagggg
     7381 gtctatttca acggagtgcc tgtccggatc gaagcactgg ccaatcaagg gtatgaattt
     7441 atcgaatggg ataacaatct cctgttaaac gatacattga acccggtatt ccagggaatg
     7501 ctggatgccg aatctgtcag tttcaatgcc atttttgaag aaatttccat cggggttgct
     7561 tctcccgaca atcagacttt aatccgggta tatcctaatc cggcgatgga ttttattcat
     7621 attctgaata atggtaacac aaaaggggct tattaccaat tgattaatgc aaatgggcaa
     7681 acaatattgg agggtaaact taacggtgat gaaacaacat tagatatccg gcatcttacg
     7741 ggggctgttt atttattaat aatcgtcgat tcagatctga accgtatttc tttcagaatt
     7801 gtgaaaatat agtttcggat ggtgactatt gatgggagat ttgatgacca cgaagtcact
     7861 gagtttacaa agaggcactt agaggaacaa taaccagcat cttcgcaaga ttcaccatgt
     7921 tgttccttcg tgtacttggt gcctcagtgg tcagtaatcc tgaaaaattg agctaatcat
     7981 tagattttca ggatgcaata agaaataaaa tttcatataa atatacatca tgaaaacagc
     8041 aatcgccagg agtatcattg aaaagcatct cgttcctgcc gacaactata tttatggttt
     8101 tgctgatctt aagaatctga tcgataagac atttggcgat tttcact
//
LOCUS       DAOPNH010000151         8113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9863_length_8113_cov_6.320179, whole genome shotgun sequence.
ACCESSION   DAOPNH010000151 DAOPNH010000000
VERSION     DAOPNH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9863_length_8113_cov_6.320179"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="rlmD"
                     /locus_tag="PKZ74_08885"
     CDS             <1..518
                     /gene="rlmD"
                     /locus_tag="PKZ74_08885"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPM92913.1"
                     /translation="FQVNVKQAEKLYSTAVEMAGFKGQETVYDLYSGTGTIACYIAKY
                     VKKVVGLEYIPSAVADAFENAGLNGIDNVDFMAGDIAKILDDDFFEKHGRPEVIITDP
                     PRSGMHEKVIRQILAARPEKVVYISCNPATQARDIQLLSEHYEVLAVQPVDMFPHTQH
                     VENIALLVKRE"
     gene            629..1357
                     /locus_tag="PKZ74_08890"
     CDS             629..1357
                     /locus_tag="PKZ74_08890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPM92914.1"
                     /translation="MVIYIATFVITIIFWLWIIRKFDRFEREPLKNILFVFIAGGLLS
                     IIPAGLFNGLFAWMIGYGFEPGSDAGIGFGRLLWFYGFVGFNEEFWKALATIILIRRM
                     KSFNEPADALVYSMTVAFGFSVIENIEYTLKLGYPVFWVRQFNAVPLHVGLAAIWGIG
                     IAKVRFSNAPSYLSRVMPYILIAAFLHFIYNIAATRIFNTALSLLIPTVIAFFLILFA
                     IRRVKKYSEEGPFRDGLGDGVSDA"
     gene            complement(1354..1929)
                     /locus_tag="PKZ74_08895"
     CDS             complement(1354..1929)
                     /locus_tag="PKZ74_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM92915.1"
                     /translation="MSDLDALKNLVLAPYILKATALIGVSRKVGGNQFRHSFSTLGIL
                     MDYKYFNDSILLKASLLHDLLEDLPETQVDEIRWIDEDASQVVDLVLEVTRRPDESKP
                     QYLQRVLETGSVRAKILKCADRISNLTDLHRDTHPDHKISDYLEQTETYILPMARQVS
                     ADMVRELTDLIAKRRKIIQRMNAGSGDGMMV"
     gene            complement(1984..2511)
                     /locus_tag="PKZ74_08900"
     CDS             complement(1984..2511)
                     /locus_tag="PKZ74_08900"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPM92916.1"
                     /translation="MLIFLIGYMASGKSTVGRMLAEELKYRFIDLDSEFEKRTGASVP
                     NYYTQFGELAFREKEREVLRLHLNDTDSVIATGGGTPSYHDNMVLMNRSGRTIFLDVP
                     VNILVRRLAGEQSRRPVIKEIPDEELASFITPHLDSRRKYYDLASMKVQVINENAEEI
                     VAQLIQRLSRTAPTL"
     gene            complement(2532..3926)
                     /locus_tag="PKZ74_08905"
     CDS             complement(2532..3926)
                     /locus_tag="PKZ74_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM92917.1"
                     /translation="MIRILLMTWLIMIGTVAAFAQKTQKTDPVQDSKPVTRILFVFDA
                     SQSMYGRWQSDLKINIARDILLKVLDSLRPMPNLEVALRLYGHQHRFPPQVCDDSKLE
                     VPFAKDNYDKIRLKLKDVIPRGTSPIAASLEQAAGDFPPCENCRNVIVLITDGIEECG
                     GDPCQASYTLQKSGIALRPFIIGIGANFEKAFDCVGIYLDASSEEKFSNALHIVVSRI
                     MNPTTCQVNLLDANNRPTETNIGMTFYDHSSGKIKYNIVHTLNSYGQPDTLNIDPMVT
                     YDIVVHTIPPVRADSVRLVPGRHTVIPVNVPQGILLLKMNSQNTNLKDITTIVRKEGR
                     SETINTQEIDQQEKYITGTYNIEVLSLPRLYRNNVDIIQSHTTTVEIPVPGIAVIQKP
                     VKGFGSLFLEKDGELELIYNFKDNDMGQESLVLMPGNYRAVFRSRYAYRSAFTIERAF
                     EVKSGTTTNVKLQD"
     gene            4070..7027
                     /locus_tag="PKZ74_08910"
     CDS             4070..7027
                     /locus_tag="PKZ74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPM92918.1"
                     /translation="MQRRLFIFKLLIIILFNGLFLRNVAQENALITLSPEERWADSVL
                     NTFSTDEMIGQLMIVRANVPNQPYFDVIERYIKDYNLGGVTFFGGHPALQALQTNRWQ
                     SMAKTPMFISIDGEWGPAMRLDSILSFPYLMTAGAAVDDSLMYFMGMEVARQCKRLGI
                     QINFAPVVDINSNPDNPVIHMRSLGEDREKVAAKGIMYMKGMQDGGLIVTAKHFPGHG
                     DTGTDSHYTLPVVPHSRQRLDSLELYPFRKLIEAGLDGIMIAHLFVPAVENSPNTPST
                     LSKAIVTDLLRGDLGFGGLIVTDALDMKGVTLNDQPGEIEVKALKAGNDILLLSANVP
                     EAVKKIKEAIEHGDLPLELIRERCRKVLLYKYKAGLNRYNPVELTGLYDDLNKPSSEL
                     VCRNIFEQSVTLVHDDHNMLPVTGLDSLKIASVSTGYGALTPFQERLGCYAPVDHFFL
                     KKEPTDEEIIALKKKIAPYNLVILSVQNTSIWGGSPYGISPKIIQLIGDISRSHRVIL
                     DLFASPYALRLIEKLPEPAAILLSYQDHPLIQDISAQAIFGGTAISGRLPVTAGNSYP
                     AGTGKTTSSMRLKYTIPEELALDRTSLAPIDSIISDAISKKAFPGCQVFAANDGKVFF
                     SKSYGYHTYTNEIPVKNSDIYDIASLTKIAATTLAVMRLADEGKIDVDRKVETYLPDF
                     ANTDKGNIVVREMMAHQARLRPWIPFYKATLKDGKPDSAFYRSTRSDKFPVRVAENLF
                     LTEAFDKIIFDSIKASSLLKSSQYKYSDLGFILLTPIIKNLTGLRLDSYAGKEFYLPL
                     GLSTTGYNPRERFDIQRIPPTENDTVFRRQLIRGDVHDQCAAMIGGVSGHAGLFSNAN
                     DLGIIAQMLLNRGIYGGNQYLSEEIIGEFTRMQFPLNDNRRGLGFDRPLPVYAEEGPC
                     CRSASQSSYGHSGFTGTYIWVDPESELTFVFLSNRVYPNAENNKLSAMNIRPKIHQVF
                     YDALEKRSVTKLK"
     gene            complement(7007..>8113)
                     /locus_tag="PKZ74_08915"
     CDS             complement(7007..>8113)
                     /locus_tag="PKZ74_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM92919.1"
                     /translation="YIFPVLVAGLVVLVYFSLRFTKKVIFAAGFFLFTVFFVLQLLPV
                     GGAVMADRYAYIPSIGIFYLAGEGFYYLWNRKQKLVPVILLSAFTIFFAITTYSRSSV
                     WKTDLSLWTDVIEHDPSVKLAYINRGNAFKNERKYDRAVADFSKAMEIDPEYALAYFN
                     RGSAYEDAKKADLALADYNKALELDPELAQAFNNRGNLFAGQNKTELALADFEQAIRL
                     DPGYSQAYNNRGTLYANANQPDLALADFTKAIELNPNNSKAYNNRANIAVNRKKYDQA
                     IEDFSRAIKLNPTFCQPYYNRGSVLSYLKKYDQAIADFTQAIAIDANFMQAYFARGQA
                     EYYSGKKAAACRDFRSAANLGFKPAADALLQLCN"
BASE COUNT         2023 a         2015 c         2013 g         2062 t
ORIGIN      
        1 tctttcaggt caacgtaaag caagccgaaa agctctattc gaccgctgta gaaatggccg
       61 gattcaaagg tcaagaaacg gtatacgacc tgtattcggg aaccggcacg atagcctgct
      121 atattgccaa atatgtaaaa aaggtagtcg ggctggaata tatcccatcg gctgtggccg
      181 atgcatttga gaacgccggg ttgaacggga tcgataatgt tgattttatg gcgggcgata
      241 ttgctaaaat cctggatgat gactttttcg aaaagcatgg aaggcctgag gtgatcatca
      301 ccgatccgcc gcgcagcggg atgcatgaaa aggtgatccg gcagattctg gccgccaggc
      361 cggaaaaagt ggtgtacatc agttgcaatc cggccacaca ggcccgcgac atccagctct
      421 tgtcggaaca ctacgaagtt ctggcggtac agcccgtcga catgttcccg catacgcagc
      481 atgtggagaa cattgctttg ttagtgaaaa gggaatagta acagattgat aatgaattaa
      541 taacagaacc ttagggctac gtcatcggaa caagcaacta acaacatcta actgctgact
      601 gctgactgct gactgcttac ttacgattat ggtaatatac attgctacat ttgtgatcac
      661 gattatcttc tggctctgga tcatccggaa gttcgaccgt ttcgagcggg agcccctgaa
      721 gaacattctt ttcgttttca ttgccggcgg attattgagt attatcccgg cgggattatt
      781 caacggttta ttcgcctgga tgatcggtta cggattcgaa ccgggatcag atgccggcat
      841 agggttcggc aggttgcttt ggttttatgg ttttgtcggg ttcaacgaag agttctggaa
      901 agcgctggcg acgatcatcc tgatccgccg gatgaagagc ttcaatgaac ctgccgatgc
      961 gctggtttat tcgatgacgg tggcttttgg attttccgtc atcgagaata tcgaatatac
     1021 tttaaaactg ggctatcccg tattctgggt acgacaattc aatgcggtgc cgttgcatgt
     1081 ggggctggcc gccatctggg gaattggaat tgcgaaagta agattcagca acgccccgag
     1141 ttacctgtcg cgcgtgatgc cgtatatcct gatcgccgcc ttcctgcatt ttatttataa
     1201 cattgccgcc acgcgcattt tcaacacggc gctcagcctg ctgataccta cagttatcgc
     1261 attcttcctg atcctgttcg ccatcagaag ggtaaaaaaa tattcggaag aagggccgtt
     1321 ccgggatgga ctgggagatg gagtctcaga tgcctaaacc atcatcccgt cgccactgcc
     1381 ggcattcatc cgctggatga tcttcctgcg tttcgcaatc aggtcggtca gttctctgac
     1441 catatcggcg ctgacctgcc gggccatcgg caaaatatag gtttcggtct gttccaggta
     1501 atcgctgatc ttatgatcgg gatgggtatc gcggtgaagg tcggtcaggt tgctgatccg
     1561 gtcggcgcac ttgagaatct tggcccgcac ggagccggtt tccagcaccc gctgcaggta
     1621 ttgcggcttg gactcgtcgg gccggcgggt cacctccagc accaggtcga cgacctggct
     1681 ggcgtcctca tcaatccagc gtatctcatc gacctgcgtc tccgggagat cctccagcag
     1741 gtcatgcaac agcgaggctt tcagtaaaat gctgtcattg aagtatttgt agtccataag
     1801 tatgcccagg gtggaaaaac tgtgccggaa ctggttcccc cccactttcc tgctcacgcc
     1861 gatcagggcg gtggctttca ggatgtaagg cgccagtacc aggttcttca gggcgtctaa
     1921 atcgctcatc ttattagttt ttgtttagaa tgacaaaatt aatggaaaaa tggagctgat
     1981 cagtcacaga gtgggagcag ttctggaaag tctttgaatc aattgagcga caatctcttc
     2041 agcattctcg ttaataacct gaactttcat cgaagccaga tcatagtatt tccgccttga
     2101 atccaggtgg ggagtgataa aagatgccag ttcctcatcg ggaatttctt taattaccgg
     2161 gcgccgtgac tgctcgccgg ccaggcgtct aaccaggata ttcaccggaa catcgagaaa
     2221 tatagttctg ccggaacggt tcatcagcac catgttgtcg tggtacgacg gagttcctcc
     2281 accggtagca atcaccgaat cggtatcgtt gagatggagt ctcaaaacct cccgctcctt
     2341 ttcccggaag gccaactcac caaattgcgt gtaataattt ggaacagagg ctcctgtgcg
     2401 tttttcaaat tctgaatcca gatcgatgaa ccggtacttc aactcctcag ccagcatccg
     2461 gccgacggtt gatttgcccg atgccatata cccgataaga aatattagca tagaaatggc
     2521 ttcagatgag gttaatcctg gagcttaaca ttggtcgttg tccccgattt gacttcaaaa
     2581 gcccgttcga tggtgaaagc cgaccggtag gcataccgcg acctgaaaac ggccctgtaa
     2641 ttcccgggca tcagcaccag cgactcctgc cccatgtcgt tatctttaaa attgtagatc
     2701 agctcaagct caccatcttt ttcaaggaac aagctcccga atcctttcac cggtttctga
     2761 attacggcta tgcccggaac cgggatttct actgtagtgg tatggctttg gatgatgtct
     2821 acattatttc tgtataacct cggaaggctc agaacctcaa tattatacgt cccggtaata
     2881 tatttttcct gctggtcgat ctcctgcgta ttgatggtct cgctccttcc ttcctttctg
     2941 acgatcgttg tgatatcttt caaatttgta ttctgggaat tcatctttaa caataaaatt
     3001 ccctgcggca cattcaccgg aataaccgta tgccggcccg gcacaagcct gacactgtcg
     3061 gctctcaccg gcggaatggt atgcaccaca atatcatacg tgaccattgg atcgatgttc
     3121 aacgtatcgg gctgaccata gctgttaagc gtatggacga tattgtattt tattttccca
     3181 gacgaatgat cgtaaaaggt cattccgata tttgtttcgg tcggccggtt attggcatcc
     3241 aggagattta cctggcaggt cgtagggttc atgatcctgg agaccacgat atgcagcgcg
     3301 ttgctgaatt tttcctcgct ggaggcatcg aggtagatgc cgacacagtc gaacgctttt
     3361 tcaaagttgg ccccgatacc aataataaat ggccggaggg cgatacccga tttctggagc
     3421 gtgtatgatg cctggcaggg gtcgccaccg cattcctcga ttccgtcggt aatcagtacg
     3481 attacattcc ggcagttttc gcaggggggg aaatcgccgg cagcctgctc cagcgacgca
     3541 gcaatcgggc tggttccccg gggaatgaca tccttcagct tgagccttat cttgtcgtaa
     3601 ttatcctttg caaacgggac ttccagtttg gagtcatcgc aaacctgggg cggaaatcga
     3661 tgctggtggc cgtatagcct caaagccacc tccaggttgg gcatcgggcg gaggctgtca
     3721 agtactttca ggaggatatc acgggcaatg ttgatcttca ggtcgctttg ccaccggccg
     3781 tacatgctct gcgaagcatc gaacacaaaa aggatgcggg tgaccggttt tgaatcctgc
     3841 acgggatccg ttttttgcgt tttctgggca aaagcagcaa ccgtgccgat cattatcagc
     3901 caggtcatta gcagaatccg aatcatcctt atcacttctc ttcccgtggg tttatcaatg
     3961 tttaagatca aattgttgaa aaaatgaaac agcgatcata tcctcccggt tcttgtgttg
     4021 tatttttgac cggagattat gtttatccat tttgaaacaa aaggaaatca tgcagcgcag
     4081 actttttatc ttcaaactct taataattat attgtttaac ggattattcc ttcgaaatgt
     4141 tgcacaggaa aacgctttaa tcactttatc acctgaagaa cgctgggccg actcggtcct
     4201 gaataccttc tccaccgacg aaatgatcgg gcaacttatg atcgtcaggg ctaatgtgcc
     4261 gaatcagcct tactttgatg taattgaaag atatataaaa gattacaatc ttggtggagt
     4321 gactttcttc ggcggccatc cggctctgca ggccctgcaa accaaccgct ggcaaagtat
     4381 ggcaaaaact cctatgttca tttccattga tggtgagtgg gggccggcta tgcgtctcga
     4441 cagcattttg tcttttcctt acctgatgac tgccggcgct gccgttgacg acagcctgat
     4501 gtacttcatg ggaatggagg tagcgcggca atgtaaacgc ctcggaatcc aaataaactt
     4561 tgccccggtc gtcgacatca attccaatcc cgacaaccct gttattcaca tgcgctcgct
     4621 gggtgaagac cgggaaaaag tagccgcaaa gggcatcatg tatatgaaag ggatgcagga
     4681 cggaggtttg atcgtgacgg ccaaacattt tcccggccat ggcgatacgg gcaccgactc
     4741 ccattatacg ctacctgtcg tgccccattc gcgccagcgg ctcgattcac tggaactgta
     4801 tcctttcaga aagctaatcg aagcaggact tgacgggatc atgatcgccc acctgtttgt
     4861 cccggccgtt gaaaattcgc cgaacacccc ttcgactctt tcgaaagcaa tcgtaaccga
     4921 cctgctgcgg ggtgacctcg gatttggcgg tttgatagta accgatgccc tcgacatgaa
     4981 aggggtgacg ctgaatgacc agcccggaga gatcgaagta aaggcgctca aagcgggaaa
     5041 cgacattcta cttctcagcg ccaatgtccc ggaagcggtg aagaagatca aagaggccat
     5101 tgaacacggt gatctgccgc tcgaactcat ccgggaacgg tgccggaaag tgctgctgta
     5161 taaatataaa gccgggctga accggtataa tccggttgaa ttgaccggat tgtatgacga
     5221 cctcaacaaa ccgtcgtctg agctcgtttg ccggaatatc ttcgaacagt ctgtgacgct
     5281 ggtgcatgac gatcacaaca tgcttccggt caccgggttg gattcgttga agatcgcttc
     5341 tgtttcaacc ggttatggtg cattaacccc cttccaggag aggcttggat gttatgctcc
     5401 ggtcgatcat tttttcctga aaaaggaacc aaccgatgag gaaattatcg cgctgaagaa
     5461 aaaaatagcg ccatataacc ttgttattct ttcggttcag aacacctcca tctggggcgg
     5521 aagcccatac ggcatttcac cgaagatcat acaactcatc ggcgacataa gccgttcaca
     5581 tcgggtcatc ctggatcttt ttgcctcgcc ttacgcgcta aggctgatcg aaaaactccc
     5641 ggagccggcg gccattctcc tctcctacca ggatcatccg ctgatacagg atatttcagc
     5701 acaggcaata ttcggcggaa cagccatcag cggcaggtta ccggttacgg caggaaacag
     5761 ctatcccgcc ggtacgggaa aaaccacctc atcgatgcgg ctgaaataca ccatccccga
     5821 agaacttgcg ctcgacagaa cctcgcttgc ccccatcgat tcgatcatca gcgacgccat
     5881 ttcaaagaaa gcttttcccg gttgccaggt attcgccgcc aatgacggta aagtgttctt
     5941 ttcaaaatca tacggatatc atacatatac aaacgaaata ccggttaaaa attcagacat
     6001 ttacgatatc gcttccctga caaaaatcgc tgcaaccacc ctggctgtta tgcgccttgc
     6061 ggatgaaggc aagatcgatg tggaccggaa ggttgaaacc tacctgcctg attttgcaaa
     6121 caccgacaaa ggaaatatcg tagtccgcga aatgatggca caccaggcac ggctgagacc
     6181 gtggatcccg ttctacaagg cgacactgaa agatggcaaa cctgattctg cgttttaccg
     6241 gagcaccagg tcagacaaat tcccggtacg ggtagctgaa aatttatttt tgacagaagc
     6301 ttttgacaag atcatcttcg acagtataaa agcatcatct ttgcttaaat ccagccagta
     6361 taaatacagc gacctgggtt ttatccttct cacacccatc attaaaaacc tgacgggact
     6421 aagattagat agctatgccg ggaaagaatt ttatctgcca ttgggtctgt cgaccaccgg
     6481 gtataatcct cgcgaacgat ttgatattca aaggattcca ccgacagaga acgatactgt
     6541 tttcaggcgt cagcttatcc ggggcgatgt ccatgaccag tgcgcggcga tgataggagg
     6601 agtttccggg catgccggat tgttctccaa tgccaatgat cttggaataa ttgcacagat
     6661 gttgctcaat cgggggattt acggtggaaa ccaatacctt tcggaagaaa tcatcgggga
     6721 atttacccgg atgcagtttc cattgaacga taaccggcgc gggctgggat tcgacaggcc
     6781 cttgccggtg tatgccgaag aagggccctg ttgtagaagc gcctcgcagt caagttacgg
     6841 ccattcgggt ttcaccggaa cctacatctg ggtagatcct gaatcagaac tgacattcgt
     6901 tttcctctcc aaccgcgttt accccaatgc agaaaacaat aagctttcgg caatgaatat
     6961 ccggccgaaa atccaccagg tattctacga tgcccttgaa aaaagatcag ttacaaagct
     7021 gaagtaacgc atcggcagcg ggtttaaatc ccaggttggc cgcagacctg aaatcacggc
     7081 aggctgccgc ttttttgccc gagtagtatt ctgcctgccc cctggcaaag taagcctgca
     7141 tgaagtttgc gtcgatggca atagcctggg tgaaatcagc gatggcctga tcgtacttct
     7201 tcaaataact gagcacactg ccccggttgt aatatggctg gcaaaaagtc gggttgagtt
     7261 tgatagcccg gctgaaatct tcgatcgcct ggtcgtactt tttccggttt actgcgatat
     7321 tggccctgtt gttataagct ttggaattat tcgggttcag ctcgatggct ttggtgaaat
     7381 cggccagcgc aagatcgggt tgattggcat tggcgtaaag ggtgccgcgg ttattgtatg
     7441 cctgggaata cccgggatcc agacgtattg cctgttcaaa atcggctaat gccagctcgg
     7501 ttttattctg cccggcaaac aggttcccgc ggttattaaa ggcttgcgcc agttccggat
     7561 cgagttcgag ggctttgtta taatcggcca gggccagatc ggcttttttt gcatcctcat
     7621 aagccgatcc gcggttaaaa taagctaagg catactccgg atcgatctcc atggctttgc
     7681 tgaaatcggc cacggcccgg tcatattttc tctcattctt aaacgcattg ccccggttga
     7741 tataggcaag ttttaccgaa ggatcatgct caataacgtc ggtccagagc gacaggtcgg
     7801 tcttccagac gctgctcctg gaataggttg ttatggcgaa aaagatagtg aacgcactta
     7861 gcaggatgac cgggactagc ttctgcttcc tgttccacaa ataataaaat ccttcgccgg
     7921 cgaggtaaaa tatgcctatt gacgggatat acgcataacg gtcggccata acggcaccac
     7981 caaccggcag caattgcagc acgaagaaaa cggtaaataa gaagaatccg gcggcaaaga
     8041 tcaccttctt tgtaaaccgt agcgaaaagt aaaccagcac aaccaaacct gccaccagta
     8101 ccgggaaaat ata
//
LOCUS       DAOPNH010000152         8095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9891_length_8095_cov_6.806965, whole genome shotgun sequence.
ACCESSION   DAOPNH010000152 DAOPNH010000000
VERSION     DAOPNH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_8095_cov_6.806965"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKZ74_08920"
     CDS             <1..980
                     /locus_tag="PKZ74_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92920.1"
                     /translation="MQGTDYQARTDNPTGTPFYGYVSYKFKDMIAIGVGVYTPFGSTT
                     KWDDNWAGRYLIRDIKLSAIYIQPTIAYNYKDKFGIGAGFVYATGSVELNKALPYSPD
                     SKVNLSGKADNFGFNAGLFFLPTEKLNIGVDYRSEISMDLEAGDATFTVPVALQTMVP
                     DTNYFDASLPLPANLDFGIAYQVNDKLTLAAEVNWIIWSSYESLDFTFENNPELLNSE
                     NPREYKDTWIPRIGGEYKLNDIFTLRAGAYFDPSPASEEYFTPETVTLNTIAFTLGAS
                     ITPLKGLDIDISYLELHGLEAEKNYSPDNFAGTYETAAFIPGIGLSYRF"
     gene            999..2345
                     /locus_tag="PKZ74_08925"
     CDS             999..2345
                     /locus_tag="PKZ74_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92921.1"
                     /translation="MKKIHLLYIVPLVLLAACKPNIDEFAPSKGNADFSSFVAVGDSW
                     TSGFADGSLYISGQEYSFPNIMNGQFKKAGGGDFLQPLMLDEYGIGLATGIPKPKLEM
                     GFHPDCKGVVGLVPAYADVPVNMDNLNPLNTGIHYDNISTPGLKTFYMGVPGMNQLNP
                     YYKRFASEGAATMNDEIPLAAPTFFSLWLGSYDALAYAIAGGADPMNPVTTKEVFAGS
                     LQQTLAALTATGAKGVMANIPDVTDFPFFHTIPYNALPLDQASADALNAGYAQVNAII
                     KGLGRTDTIHFKSGANAVIIADATLPWGFRQVKPTEFLLLSLPQDSLKCAGWGSQKPI
                     PGQYVLDEAEIAITKNAVDEYNSQINLLIKDLPDVALVNMYTIMKEITETGLVFNHVN
                     VNARFVTGNFYSTDGLNPTPMGSAVIAHYFIKTINESFQANIPQVIVSEFPAVKLP"
     gene            2541..3809
                     /locus_tag="PKZ74_08930"
     CDS             2541..3809
                     /locus_tag="PKZ74_08930"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPM92922.1"
                     /translation="MRLFISLLLVISALSAGAQFNRYFENATLRFDYFHSGSVEAEYV
                     VPDELIREGAWAGPRKNLIDPFDYGAFKIMVYDSITGKLIYTRGFSSLFIEYQATEQA
                     LTECGNFQESILMPFPKRTVKLEYYSRGKEMVWTRQFETFINPVKDEIRIKPGAGYIT
                     EDILFSGKPKKKLDLVFLPEGYTAAEMDKFRTDCNRFTDFLFQTEPFGEYRKKINIRA
                     VMAPSEESGTDIPTDSVFKNTLLGAGFNTLNTERYLMATDFKIVRDVASAVPYDQIII
                     MVNSPQYGGGGIYNFYAISTVDNIYSGFVFTHEFGHSFAGLGDEYSDSGSAAGILYLE
                     NAEPWEPNITTLADFESKWKDMLPESTPVPTPATEEFNGKLGVFEGAGYQAKGAYKPY
                     LDCSMNMIRFNNFCPVCQRAMRGMISFYGR"
     gene            3915..4820
                     /locus_tag="PKZ74_08935"
     CDS             3915..4820
                     /locus_tag="PKZ74_08935"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPM92923.1"
                     /translation="MLKKLSDLHDLVKEKQQAKRLVVAVAHDEHSLEAVAMAAKKKYI
                     EAILVGNAAEIKKIAEKNKKIDISQFEIINEINDSEAVAKAVKLVHYGEADILMKGNV
                     GTATLLKGVLNKEWGLRKNEVLSHIALFEVQVYHHKLISLTDVAMNIAPDLNTKIGII
                     NNSVEFLKKLGVETPKVAVLGAVELVNESMPATIDAALLSKMAERGQIKGCIIDGPLA
                     FDNAISAESAKHKGIKSIVAGDADLLLLPDIEAGNVLYKAFVFFAKAKVAAVVLGARA
                     PIVLTSRSDSEESKLDSIVLAAAIA"
     gene            4912..5991
                     /gene="buk"
                     /locus_tag="PKZ74_08940"
     CDS             4912..5991
                     /gene="buk"
                     /locus_tag="PKZ74_08940"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPM92924.1"
                     /translation="MEDVYIIAINPGSTSTKIAVYRNKECIFEENIKHSADDLAPFEK
                     ITDQFEFRKRVIMEKLNGTRIEKDKVRVIMGRGGLVYPIESGVYRINEKMKHDLVHSP
                     VGEHASNLGGFIAEDLAKDFPNAEAFICDPVVTDELDEIARIAGHPEFTRRSVFHALN
                     QKATARRHATAVGKKYEELNLIVAHLGGGISIGAHRHGRVVDVNHAIGGEGPFSPERA
                     GSVSVIDAVKLAYSGKYSEKEFTKMLIGKGGLCAHLGTNNAYEVEQRVKNGDEHAALV
                     YEAMAYQVAKFIGMKAVVLKGKVDGILITGGIAYDKWFVGKIREYADWIGPFFIYPGE
                     NEMEALAMNALRVINGEVEVKEYQN"
     gene            6047..>8095
                     /locus_tag="PKZ74_08945"
     CDS             6047..>8095
                     /locus_tag="PKZ74_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM92925.1"
                     /translation="MRNLFLLAAMLLLYPVLSISQTFIYSDNKDNQGFILLSSSDEKA
                     ELTFSVAAWSLETVTVNRAEMQKVTLPGHFLPNDEGMPDLPGSGRYMAIPQGSTASLV
                     IRNMETEILQNINMAPAPRIPKTTENGDLFYAINEQVYSTDAFYPAEPVKLSEAKQIR
                     GVDVVMLGITPFQYNPVTKELIVIKNLEIEVEFSGGNGHFGDDRLRSRWWEPVLADMV
                     MNYEELVPFGKISRRSAVGSQQPVGSSQQSAVGSRQAAGSRGETGFEYLIICPDDPAF
                     LAWADTIRVFRNKQGISTGVVTTTQTGGNSVNAIRTYINTAFNTWDIPPAAILILGDY
                     GTTGSTVVSPIWGNYCASDNIYGDVNNDDLPDIVMARIFAQNEAHLALMVQKFIDHEI
                     NPPVDPDYYDKPVTALGWQTERWFQICIETVGGFFRNVLGKEPVRINAIYDGDPNVDP
                     WSYAPNTSTILNYFGPNGLGYLPASPSTLGYWIGGTSGKISNAINNGTFMVLHRDHGY
                     EQGWGEPAYSNSSMASLDNEHTTFVYSVNCLTGKFNIGGECFAEGFLRYPKRAYGIIA
                     ASEVSYSFVNDTYVWGAMDHMWPEFMPEYGTTPETRGLFPAFSNAAGKFFLEQSSWPY
                     NTDNKEVTYHLFHHFGDAFTTIYSEVPQELLAVHNDVLIGGAATFRVHTEADALVALS
                     I"
BASE COUNT         2078 a         2113 c         2059 g         1845 t
ORIGIN      
        1 agatgcaggg aacggattac caggcacgga ccgacaaccc gacgggcacg ccattctacg
       61 gctatgtctc ctacaaattc aaagacatga tcgctatcgg cgtaggggtt tatactcctt
      121 tcggcagcac gacgaagtgg gatgataact gggcgggccg gtacctgatc cgggacatca
      181 agctcagcgc catttacatt cagccgacca ttgcatacaa ttacaaagac aagttcggga
      241 tcggcgccgg gttcgtgtat gccaccggca gtgtggaact gaacaaggct ctcccctaca
      301 gccccgattc aaaggtcaat ctttcgggaa aagcggacaa cttcggtttc aatgcaggtc
      361 tctttttttt acccaccgag aagctgaaca tcggagttga ttaccgctcc gaaatctcaa
      421 tggacctcga ggccggcgac gccactttta ccgtaccggt tgccctccag accatggttc
      481 ccgacaccaa ttatttcgac gcctccctgc ccttgccggc caacctcgat ttcggcatag
      541 cttaccaggt caacgacaag ctgacactgg cggcagaggt gaactggatc atctggagca
      601 gctacgaatc acttgatttt acatttgaaa ataatcccga actgctgaat tccgaaaatc
      661 cgagggaata caaagacacg tggattcccc gcatcggagg cgaatataag ctgaacgaca
      721 ttttcaccct ccgcgccggg gcctatttcg atccctcccc ggcaagtgag gagtatttta
      781 ctcccgaaac ggttaccctc aataccatcg ccttcacttt aggcgcctcc attacaccgc
      841 tgaaagggct tgatattgat atttcctacc tcgaactcca cgggctcgaa gcagaaaaga
      901 attacagccc cgataatttt gccggaacct acgagacagc cgctttcatt cccggcatcg
      961 gcctcagcta ccgtttttaa cctaaaatac tcacgaatat gaaaaagata catttgcttt
     1021 atatcgttcc gctggtattg ctggcagcct gcaagccgaa catcgacgaa tttgcccctt
     1081 cgaagggaaa tgctgatttc agcagctttg tggccgtcgg ggattcctgg acatccgggt
     1141 ttgccgatgg ctccctgtac atatccgggc aggaatactc cttcccgaac attatgaacg
     1201 gacagtttaa aaaagccggg ggtggcgatt tcctccagcc tctcatgctt gatgaatacg
     1261 ggattggcct ggcaacaggc atcccgaagc caaagctcga aatgggattt catcccgatt
     1321 gcaaaggagt tgtcggactg gtcccggctt acgccgatgt gcctgtcaat atggataacc
     1381 tgaaccctct gaataccggc atccactacg acaacatctc cacacccggg ctgaagacgt
     1441 tctatatggg tgtacctggc atgaaccagc ttaatccgta ttataaaagg tttgcttcgg
     1501 aaggggcagc cactatgaat gatgagatac cactggcagc ccccaccttc ttctcgctct
     1561 ggctgggcag ctacgatgct ttggcctacg cgatcgcagg tggcgccgac ccgatgaacc
     1621 cggtaacgac aaaagaggtg ttcgcaggat cattgcaaca gaccctggcc gccctgacgg
     1681 caaccggtgc caaaggcgtg atggccaaca tccccgatgt gaccgatttt ccgttcttcc
     1741 acaccatccc ctacaatgcc cttccgctcg accaggccag cgccgacgcg ctgaatgccg
     1801 gatacgccca ggtaaatgcc attatcaagg gtttgggaag gaccgatacg atccatttca
     1861 agtcgggtgc aaatgcggta attattgccg atgctacctt gccctgggga ttccgccagg
     1921 tcaaaccgac ggaattcctg ttgctgagcc tgccgcagga ttcgcttaaa tgcgccggat
     1981 ggggaagcca gaagcccatc cccggccagt atgtgctgga tgaagccgag atcgccatca
     2041 cgaaaaatgc cgtcgacgaa tacaattcgc agatcaacct gcttattaaa gatcttccgg
     2101 atgttgcgct ggtgaatatg tatacgatca tgaaagagat caccgaaacc gggctggtct
     2161 tcaaccatgt gaatgtcaac gcccgatttg tcaccggcaa tttctattcc accgacggtc
     2221 tgaatcccac gcccatggga agcgccgtga tcgcccacta tttcatcaaa acgatcaacg
     2281 aatcattcca ggcgaatatt ccacaggtga tcgtgagtga gttcccggcg gttaagctac
     2341 cgtaattaac tatctttgct gaggctgttt caaaagtcta ttatttaacc acgaaggcac
     2401 acaaaggatc ttcacaaagg cgcactaagt aattgattaa caggaataga tctttgtgtt
     2461 ccttagtgaa atccttcgtg cgcctttgtg gttgaacact tttagacaac ctcactataa
     2521 aaattaaaat atgacaatcc atgcgtctgt ttatctcact attgttagta atttcagcac
     2581 tttcagccgg tgcgcagttt aacaggtatt tcgaaaatgc caccctccgg ttcgattatt
     2641 ttcattcggg gagtgtcgag gcggaatatg tggttcccga tgaattgatc cgggaaggcg
     2701 cctgggccgg gccacggaaa aacctcatcg atcctttcga ttacggggcg ttcaagatca
     2761 tggtttatga ctcgataaca ggtaagctga tttacacgcg cggcttttcc agccttttca
     2821 tcgagtacca ggccaccgaa caggcgctga cggagtgcgg caatttccag gagagtatcc
     2881 tgatgccgtt tccgaaacgg acagtgaaac tggaatatta cagccgcggg aaggagatgg
     2941 tgtggaccag gcagtttgag acatttatca atccggtgaa agacgagatc aggattaaac
     3001 cgggcgccgg gtatattacg gaagatattt tgttttccgg caaaccgaaa aagaagcttg
     3061 atcttgtttt tttgcccgaa gggtacacag cagcagagat ggacaaattc cgcaccgatt
     3121 gcaaccggtt cacggatttc ctttttcaga cggagccttt cggagaatac cggaaaaaga
     3181 tcaatatccg ggcggtcatg gcgccgtcgg aagaatccgg caccgatatc ccgaccgact
     3241 cggtgttcaa aaatacattg ctgggcgccg gtttcaacac cctcaacacc gagcgctacc
     3301 tgatggcgac agacttcaag attgtccgcg atgtagcatc cgctgtacct tacgaccaga
     3361 tcatcatcat ggtgaacagc ccgcagtatg gcggtggcgg catctataat ttctatgcca
     3421 tcagtacggt tgacaatatt tactccggat ttgtatttac gcacgaattc ggtcattcgt
     3481 ttgccgggct gggtgatgag tacagcgact ccggatcggc agccggaatc ctttacctgg
     3541 aaaatgccga gccctgggag ccgaacatta ccactctggc tgattttgaa tcgaagtgga
     3601 aggatatgct tcctgaaagt actcctgttc ccaccccggc gactgaagaa ttcaacggga
     3661 agctgggtgt tttcgaaggg gccggctacc aggcgaaagg tgcatataag ccttacctcg
     3721 actgctccat gaacatgatc cggttcaaca acttctgccc ggtctgccag cgggcaatgc
     3781 gggggatgat ttcgttttac ggtcgttagt ctaacgacta atttttgcta atccaactta
     3841 ttattgtact tttgcgctgt tattttttta acaattacat cgaacttatt attcttcaat
     3901 aaattacagg aaaaatgcta aagaaattat cggatcttca tgatctagtc aaagagaaac
     3961 agcaggcaaa aaggctggtg gttgcggttg cacacgatga acattcactg gaggcagtcg
     4021 cgatggcggc aaagaaaaag tatatcgaag caatcctggt ggggaacgcg gcagagatca
     4081 aaaagatagc cgaaaagaac aaaaagattg atatcagcca gtttgagatc atcaacgaga
     4141 tcaatgactc ggaagcggtt gccaaggcgg tgaagctggt ccattacggc gaagcggata
     4201 ttttgatgaa aggaaacgtg ggcacggcaa ctcttctgaa aggtgtactg aacaaggaat
     4261 ggggattgcg gaagaatgaa gttctctcgc atattgccct ttttgaggtc caggtttatc
     4321 accacaagct catttcgctg accgatgtgg ccatgaacat cgctcccgac ctgaatacca
     4381 agatcggtat catcaacaat tccgtggagt ttctgaagaa gctcggcgtg gaaaccccga
     4441 aggtggcggt tctcggcgcc gtggagctgg tcaacgagag catgccggcg acgatcgacg
     4501 ccgccctgct ttcgaagatg gccgagcgcg gacagatcaa aggttgcatc atcgacgggc
     4561 cgctggcctt cgacaatgcc atcagcgccg aaagcgccaa gcacaaaggg atcaaaagca
     4621 ttgtggccgg tgatgccgac ctgctgttgc tccccgatat cgaagcgggt aacgtgcttt
     4681 acaaagcgtt cgttttcttt gccaaagcga aagtggctgc cgtcgtgctc ggcgcccgtg
     4741 ccccgatcgt gctcacttcc cgctccgact cggaagaatc gaagctcgac agcatcgtcc
     4801 tcgccgcagc aatcgcttga cagtcagtag ctagcggtca gcagtctgca gtaattaaga
     4861 attaaaaatc aagaattaaa aattaagaat taaaaattaa gaatttcttt cgtggaagac
     4921 gtttacatca tagccatcaa cccggggtca acatcgacca aaatagcagt gtatcgcaat
     4981 aaggagtgta tttttgaaga gaacatcaag cacagcgccg acgacctggc gccgttcgaa
     5041 aagatcaccg accagtttga attcaggaag agggtgatta tggaaaagct caacggcacc
     5101 cggatcgaaa aggataaagt gcgggtgatc atgggccgcg gcgggctggt ttacccgatc
     5161 gaatcgggtg tttaccgcat caacgaaaaa atgaagcacg atctggtgca cagcccggtc
     5221 ggcgagcatg ccagcaacct gggcggattc atcgcggaag acctggccaa agatttcccg
     5281 aatgccgaag cctttatctg cgatccggtg gtaaccgatg aactggatga aatcgcccgc
     5341 attgccggcc atcctgaatt tacccgccgg tccgttttcc atgcgttgaa ccagaaagcg
     5401 acggcccggc gccatgccac cgctgtcggt aaaaaatacg aagagctcaa cctgatcgta
     5461 gcccaccttg gcggcgggat cagcatcgga gcccaccgtc acggtcgggt tgtcgatgtg
     5521 aaccacgcca tcggcggcga aggtccattc tcgccggaac gggccgggag cgtttcggtg
     5581 attgatgcgg taaaactggc ttacagcgga aaatacagcg aaaaagaatt caccaaaatg
     5641 ctgatcggaa agggagggct ttgtgcacac ctcggcacca acaacgccta cgaagtcgaa
     5701 cagcgcgtta agaacggcga tgaacatgcc gcactggttt acgaagccat ggcctaccag
     5761 gtagccaaat tcatcggcat gaaagcggtt gtgctgaaag ggaaagtcga tggtatcctg
     5821 attaccggcg gtattgccta tgacaaatgg tttgtcggaa agatccgcga atatgccgac
     5881 tggatcggcc cgttcttcat ctatccgggt gaaaacgaaa tggaagccct cgccatgaac
     5941 gccctccgtg taatcaacgg agaggtggag gtaaaggaat atcagaatta aaagatttta
     6001 ttatttttac cccccgataa gcgattcatc acttaacctg tctttcatga gaaatttgtt
     6061 cctgcttgca gcaatgctgt tgctgtatcc tgttttatcc atatcacaga catttatata
     6121 ttcggataac aaagataatc aaggatttat acttctttcc tcttcggatg aaaaggctga
     6181 attaaccttt tccgtcgccg cctggtcgct cgaaacagtt accgtcaacc gggcagagat
     6241 gcaaaaagtg accctgccgg gccatttcct cccaaacgat gaagggatgc ctgacctccc
     6301 gggatcggga cggtacatgg ccatcccgca gggatcgaca gccagccttg tgatccggaa
     6361 catggaaacc gaaatcctgc agaatatcaa catggctccg gccccccgga ttcccaaaac
     6421 cactgaaaac ggtgatcttt tctatgcaat caacgaacag gtctattcaa ctgacgcttt
     6481 ttatcctgcc gaaccggtaa aactttcgga agcgaagcag atcaggggcg tggatgtggt
     6541 catgctcggg attaccccgt ttcagtacaa cccggttacc aaagagctta tcgtcataaa
     6601 aaacctggag atagaggtcg aattttcagg gggtaacggc cattttggcg atgacaggct
     6661 tcgcagccgc tggtgggagc cggtgctggc ggatatggtg atgaactatg aggagttggt
     6721 accattcggg aaaataagtc ggcggtcggc agtcggcagt cagcagccgg tgggcagcag
     6781 tcagcagtcg gcagtcggca gtcggcaggc agcaggcagc agaggcgaaa ccgggtttga
     6841 gtaccttatc atctgccccg atgatcccgc cttcctggcc tgggccgaca caatccgggt
     6901 attcaggaac aagcagggca tctcaaccgg tgtggtaacc accacccaaa cggggggaaa
     6961 ttcggtcaat gccatccgga cctatatcaa caccgctttt aatacctggg atatccctcc
     7021 ggcagcgatc ctgatcctgg gtgattacgg aacgacaggt tcgacggtcg tttcaccgat
     7081 ctgggggaat tattgcgctt ccgataatat ctatggagat gtgaacaacg acgacctgcc
     7141 tgatatcgta atggccagga ttttcgcaca gaatgaggct catctcgcat tgatggtgca
     7201 aaaattcatt gaccacgaaa tcaatcctcc ggtcgatccg gattactacg acaagccggt
     7261 tacggcgctg ggctggcaaa cggaacgctg gttccagatc tgtatcgaaa cggttggcgg
     7321 ctttttccgg aatgtgctcg gcaaagaacc ggtccggatc aatgccattt acgatggcga
     7381 tcccaacgtt gatccgtggt cctatgctcc caataccagc accatcctta actatttcgg
     7441 cccgaacggg ctgggttacc tcccggccag cccctccacc ctcggctact ggatcggcgg
     7501 cacatcgggc aagatcagca acgccatcaa caacggcacc ttcatggttc tccaccgcga
     7561 tcacggctac gaacagggct ggggcgaacc ggcttacagc aacagcagca tggcctcact
     7621 cgacaatgag cacaccactt tcgtttattc ggtcaattgc cttaccggaa aattcaatat
     7681 cggcggggag tgttttgcgg agggcttctt acgctatccc aaacgggcct acggtatcat
     7741 cgccgcctcc gaagtgtcgt attcctttgt gaacgacacc tatgtctggg gtgccatgga
     7801 ccatatgtgg ccggaattca tgcccgaata cggcactaca cctgaaacaa gagggctgtt
     7861 tcccgcattc agcaatgccg ccgggaaatt tttcctggaa caatcttcct ggccgtataa
     7921 tacggataac aaagaagtta cctatcatct cttccaccat ttcggcgacg ccttcaccac
     7981 tatctattcc gaggtaccgc aggaactcct ggctgttcac aacgatgtat taatcggagg
     8041 cgcggccaca ttccgggtcc ataccgaagc agatgcactg gtagcgctca gtatc
//
LOCUS       DAOPNH010000153         8060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9952_length_8060_cov_5.776640, whole genome shotgun sequence.
ACCESSION   DAOPNH010000153 DAOPNH010000000
VERSION     DAOPNH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9952_length_8060_cov_5.776640"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKZ74_08950"
     CDS             complement(<1..541)
                     /locus_tag="PKZ74_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92926.1"
                     /translation="MYEDIFFKLACELKRAIIIILLTESQNPLIYKFLKMRNLHLAVK
                     TKTRPERPWKRSINFIPLFILLVLISFSGKGYGQPLLVENFDYTVGTYITANGWANHS
                     TGVNPITVTASTITYPGYLSSGIGAEVTMAVSGEDDNKTFPQQTSGTIYAGFLVNVTS
                     ATTGGDYFFHVGATTIGSTF"
     gene            642..4028
                     /locus_tag="PKZ74_08955"
     CDS             642..4028
                     /locus_tag="PKZ74_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPM92927.1"
                     /translation="MTIIILIACYQCYSQVYTPGFLSRISGEELSYHAPQPDATTSLL
                     VRSEDSTRFIEWESAMIAETGYSKQEYGSNKPGTWNPEYGTFLLLAGIDVNPADPRSW
                     KVYVNDRHLFTISSPLDTIQKTITWPGPDGSSLVFKTKEVDKYGDFMGYLYCNLPSSM
                     LEAGKPVRFRVVGESAGSRTWFMVFEYETNDRVRLIPEQAVVKGDKGNYQVLRAEIVR
                     YAEPVEVKITVAGKKLNRNLDFGYNVLYLPVPEITAETAFDFVIKAGKTLISRESFTI
                     APVIPRTIYLLHHSHNDIGYTHVQPEVEEIQWQNLDRALILSDSTKDFPAGSGLKWCT
                     EVIWAFESWYEKADPAKKEQIRQALKDGRIELNALFANELVSLCGPEELDRLLEAGRR
                     IAKECGVDLNSAMITDIPGWSWALVPAMARSGVKYFSIGTNRGHRIGSILEEWGDRPF
                     YWVSPSGEEKILCWIHGEGYSLFHTGLAYSIIRKRLQEGLLFGYLNKLNQTNYPYEEV
                     MLRYNIGSDNGPVDETLPMAVREWNERYVTPKIVISTVGEAFSAFEKKHGPGLQEFQG
                     DLTGYWEDGAWSTARETHQNRRNASRLIQAQALWAMYNPENYPVDEFYAAWQNVLLYD
                     EHTWGSWNSISEPDSPFTLQQWEIKRSFAEKAEQQSRRLFFGAMTSKPLGGPATDAIE
                     VINTCGWPRSGLVTIYGGPNPEAVGLADDTGKLIETQLLSDGNIAFLAKSVPALGSKI
                     YKITHTQGQKSQIPSTKNQTNSKSINLETLAYSLSIDSLTGAISSFIWHPGETPSQSA
                     RLHLEKPDGVSSVPSSLVDLSVYSGLNEYLYTSGRDPEKSSTGKLLEIRTIDDGPLVK
                     TFSMELEAPGCESLNVTIRITDALGQVEIINTVRKKKVVEPEAVRFIFPWNVAGGEMT
                     YDLAYGFCRPEKDQLPGANRNYLAMEHWIDISGKQGGVTVVCPDAALFETGKPGMDAI
                     VTGWVDTLPSSQAFLSYAMNNYWETNFAASQEGPCEFSYVVYPHEAFDPALAEQRAIG
                     YRQPLITRKGGGWMKARPSLVQFMNRNLVITSVKPLDNGKALLVSVYNAGMKKEMPRL
                     QNQGKLQVSDPDGINGKPLAPAESIPAGGIKHFIISAEHW"
     gene            4205..5038
                     /locus_tag="PKZ74_08960"
     CDS             4205..5038
                     /locus_tag="PKZ74_08960"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92928.1"
                     /translation="MKKLYKTLTLTAIILALTAFTGRSQNAWLNEIHYDDFGTDTVEF
                     IEIVIQDVENFDLSSFEVVLYNGNNGESYDTKSLDEFTVGQVENVFTFFYRVYPTNGI
                     QNGEADGLALVYDGTVIPGQFLSWEGTLTAVDGPASGMTSVDIGVAEAGELEGKSLQL
                     SGESNEYDGFTWQMPSEETMGDVNNDQFFLHFGLKEPGLSHVDFFPNPGNGEGQIFNP
                     FSNNLVLNVFSVDGRMIRQITVKPGENLIRLENLASGIYLINLQEENGRPIGNDKLVI
                     R"
     gene            complement(5042..7321)
                     /locus_tag="PKZ74_08965"
     CDS             complement(5042..7321)
                     /locus_tag="PKZ74_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92929.1"
                     /translation="MIRAIHQFLRQGMALLFAVTAFQANINAQDAEIRGRITDAADSN
                     PLAGVNIVLDSLNGAASDAQGQYSLKVKPGSYVLNYRFVGYEPYRVSVTLKENEILTK
                     DIQLSQSAVLLNTAVISASRYEQRLSDVTVSMEIIKPTFIEKQNANQLDEALRLIPGV
                     DVIDGQANIRGGSGYSYGAGSRVMILVDELPMLTGDVNEVKWSFIPVENIGQVEVIKG
                     ASSALYGSSALNGVINVRTAAPGDKPETTVSLSQGVYTRPARKELSWWWDGVPLYSSV
                     KASHMRKAGPVDLTFGLNGFGDQGYRTDNYQYYGRASAGIRYNPPGVKGLSAGLRASI
                     QWQESSDFLLWTDADSGAFVQNPVSVSPTNGIRFNVDPYVVYFDDKKGEHALRTRYYK
                     VQNTFDEDPDKDNGSGYYFGEYQYQKQFRYNIHLAAGLAASYTDGRSNLYGNHTGSSF
                     AAFAQFDKRFFDRLSASLGLRWERNRLDAEKAEAKPVVRAGISYKAAGSTFLRASFGQ
                     GYRFPSMAEKYTATSLGSLNIFPNPDLTSETGWSAEIGIRQGFQLGSLSGSLDVAGFW
                     TEYQDMMEFTFGLYLPDSNTLPTLDHIGFKSVNIGVARIAGIDISLNGQVKTGAFLLS
                     YFAGYTYLDPIDHSVDSIENEILKYRYRHSAKGNAEVEYRHYNLGITINYQSFTERID
                     EAFETVILGQEFFPGLKDYREANNKGAVVIDIRTGYQLTASSEISLIIKNIFNKEYMG
                     RPGDIQPPRTIALQYVLKI"
     gene            complement(7342..>8060)
                     /locus_tag="PKZ74_08970"
     CDS             complement(7342..>8060)
                     /locus_tag="PKZ74_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92930.1"
                     /translation="PLSGRSQCEPMTAEECPDPEENGQVCPDSLEVAYLNQFYSQVAT
                     IKTPAVYYQPPDSTVITIHHVKLKEVGNLPPGLTWQSDSPDSVFMAGEFNCVVMEGQP
                     EAAGIYPLAIKVGVSVIPFPGWPPIEVANVTDSTSLSILVVDDSGIHESKEIFAGQNF
                     PNPFVDMTVIPYSTSHTGEATFEVFTLAGQRVHTAAYPVNRGENELIYNGFTLPKGSY
                     FYLVRDGIGLTGGIMVKSGR"
BASE COUNT         2014 a         2046 c         2088 g         1912 t
ORIGIN      
        1 tgaaggtcga gcctattgtt gttgctccga catgaaagaa ataatcgcca cccgttgtag
       61 cagaagtaac attcaccaga aaacctgcat aaattgttcc tgaagtttgt tgtggaaatg
      121 tcttgttgtc atcctcaccg cttacagcca ttgtaacttc cgctccgata cctgatgaaa
      181 gatatcctgg gtatgttatc gttgaggcag taaccgtaat ggggttaact cctgtactgt
      241 gatttgccca accgtttgcc gtaatataag tccctactgt ataatcaaaa ttttccacca
      301 ggagtggctg tccgtatcct ttccctgaaa aggagataag cacaagcaaa atgaataatg
      361 gaataaaatt tatactcctt ttccacgggc gctccggcct cgtctttgtc ttaacggcaa
      421 ggtgtaaatt tctcatcttt aagaatttat agattaatgg attttgggat tctgttagca
      481 ggataataat tatcgctctc tttaattcgc atgctaattt aaaaaaaata tcttcataca
      541 aacctacgcc attcccgaaa tatttatatt aataaatggt tatttttacc ggtttgattg
      601 cccgatttaa ttcctgattt tcgcagacta tgaaaaatac aatgacaatt atcattctta
      661 tagcctgtta ccaatgctat tcccaggttt acacccccgg tttcctttca cggatcagcg
      721 gtgaagagct atcctaccac gccccgcagc ccgatgccac cacctcgctg ctggtccgct
      781 ccgaagacag cacccgcttc atcgaatggg aatccgctat gattgcggaa actggatact
      841 cgaaacagga atatggaagt aataaacctg gaacctggaa cccggaatat ggaactttcc
      901 tgctcctcgc cggcatcgac gtcaaccccg ccgacccccg ctcctggaaa gtgtatgtga
      961 acgacaggca tctgtttacg atctcgtcac cccttgatac catccaaaaa accatcacct
     1021 ggcccggacc ggatggatcc agcctggttt tcaaaacaaa agaggtcgat aaatacggcg
     1081 atttcatggg ctatctctac tgcaacttac cctcttcaat gctggaagca ggaaaaccgg
     1141 tgcggttcag ggtcgtcggt gaaagtgcag gcagccgtac ctggtttatg gtttttgaat
     1201 atgagacgaa tgaccgggtg aggctgatcc ccgaacaggc tgttgtgaaa ggcgacaaag
     1261 gcaactacca ggtattgcgg gccgagatcg tcaggtatgc ggagccggtg gaggtaaaga
     1321 tcactgttgc cggcaaaaag ctgaacagaa atctcgattt cggttacaat gtcctctacc
     1381 tacctgtccc cgagatcacg gccgaaacag ccttcgactt tgtcatcaag gccggaaaaa
     1441 cactcatctc cagggaatcc tttaccatcg cacccgttat cccgcggacg atctatctgc
     1501 tccatcattc gcacaacgac attggctaca cgcatgttca gccggaggtg gaggagatcc
     1561 aatggcagaa tcttgaccgg gcgctgattc tgagcgattc gacaaaagat tttccggcag
     1621 ggtcaggtct caaatggtgc accgaagtga tatgggcctt tgagagctgg tatgagaaag
     1681 ccgatccggc caaaaaagaa cagatcaggc aagcattgaa agacgggcgg attgagctga
     1741 atgcattatt tgccaacgaa ctggtgtcgc tctgtgggcc ggaagagctc gacaggctgc
     1801 tggaagccgg gcgccggatt gcgaaagagt gcggcgttga tctgaactcg gctatgatca
     1861 ccgatatccc ggggtggtcg tgggctttgg tgccggcaat ggcccgaagc ggagtaaaat
     1921 acttctctat tggcacaaac cgtggccacc ggatcggcag cattctcgaa gaatggggcg
     1981 accggccgtt ttactgggtc tccccttccg gggaagaaaa aatattatgc tggatccatg
     2041 gcgaagggta ttctctcttt cacaccggac tggcatacag cattatacgt aagcgactcc
     2101 aggaaggcct cctcttcggt tacctgaata aacttaatca aacgaattat ccctacgagg
     2161 aggtgatgct gcggtacaat atcggctccg acaacggccc ggtagatgag acgctgccca
     2221 tggctgtcag ggagtggaat gagcggtatg tgacgccaaa gatcgtgatc tcgactgtcg
     2281 gagaagcatt ctctgctttt gaaaaaaagc acgggccggg attacaggaa ttccagggcg
     2341 accttacggg ttactgggaa gacggcgcct ggtcgacggc acgggagacc caccagaacc
     2401 ggcggaatgc ttcccgtttg atccaggcac aggccttgtg ggcgatgtac aacccggaga
     2461 actaccctgt ggacgaattc tatgctgcct ggcagaatgt gttgctgtac gacgagcata
     2521 cctggggcag ttggaacagc atcagcgagc ccgattcacc ttttacgctg cagcagtggg
     2581 agatcaagag atcttttgca gagaaagcgg aacagcaaag ccggaggttg tttttcgggg
     2641 cgatgaccag caagccgctt ggagggcctg caacggatgc catcgaggtg atcaacacct
     2701 gtggctggcc ccgcagcggg ctggtaacga tctatggcgg accgaatccg gaagccgttg
     2761 gcctggcaga tgatacaggg aaacttatcg aaacgcagct cctttcggat gggaacattg
     2821 cattccttgc caaatccgtt cccgccctgg ggtcaaaaat ctataaaatc acccatacgc
     2881 agggtcaaaa atcccaaatt ccaagcacca aaaaccaaac aaattccaaa tccataaatc
     2941 tcgaaacgct cgcttattcg ctttccatcg attctctgac gggagcaata tcatcgttta
     3001 tatggcaccc tggcgagact ccatctcaat ctgcgagact ccatctcgaa aaaccagatg
     3061 gcgtctcatc cgttccatcc agtctcgtcg atctttctgt ttactccggc cttaacgaat
     3121 atctctacac ctcaggaaga gatcccgaaa aatcatccac cggaaaatta ctagaaatca
     3181 ggacgatcga cgatgggccg ctggtaaaaa cgttcagcat ggagctggag gcgccgggct
     3241 gcgagagcct gaacgttacc attcgcatca cggatgccct cggccaggtt gaaatcatca
     3301 atacggtgcg taaaaagaag gtcgttgagc cggaggcggt gcgcttcatc ttcccgtgga
     3361 atgttgccgg cggcgagatg acatacgacc tggcttatgg cttttgccgg cctgaaaaag
     3421 accagttacc cggagcgaac cggaactacc tggccatgga gcactggatc gatatatcgg
     3481 gcaaacaggg aggagtgact gtggtttgtc cggatgcagc attatttgaa accggtaaac
     3541 cggggatgga tgcgattgtg acgggctggg ttgataccct cccctcctcc caggcttttc
     3601 tttcctatgc catgaataac tactgggaaa ccaacttcgc tgcaagccag gagggcccat
     3661 gcgagttcag ctatgtggtc tatccgcatg aagccttcga tccagccctg gccgaacaga
     3721 gagccatcgg ttaccgccag ccgctgatta cccgcaaagg gggcggatgg atgaaagcaa
     3781 gaccttcgct ggttcagttc atgaacagga acctggtgat tacgtcagtg aaaccgcttg
     3841 ataatggaaa agctttgctg gtttcggttt acaatgcagg gatgaaaaaa gagatgcccc
     3901 ggctgcagaa tcaaggaaaa cttcaggttt cagatcccga cggaattaac gggaaacctc
     3961 tggctccggc agaatccatc ccggcaggcg gaataaagca ttttataatt tcagctgaac
     4021 actggtaatt aagaaaagaa attcgtgttc attcgcttaa attcgcgttt attcgtgtgc
     4081 caatgatcga acgcgaatta acgcgcatga aacgaatttg cacgaatttt tgaaactgta
     4141 agtaatgttg ttttatcttt gagatcggtc ttagaaccgg caagaacata aaattccaaa
     4201 tcaaatgaaa aaactttaca aaaccctgac cctcaccgcc atcatccttg ctttaaccgc
     4261 ctttaccgga cggtcacaaa atgcctggct gaatgagatc cactacgatg atttcgggac
     4321 cgacaccgtc gaatttatcg agatcgtgat ccaggatgtg gaaaatttcg acctttcctc
     4381 attcgaggtt gtgctatata acggcaataa cggcgaatcc tacgacacaa aatcgctgga
     4441 tgagttcacc gttggccagg tagagaacgt gtttacattt ttctatcgtg tttacccgac
     4501 caacgggatc cagaacggcg aagccgacgg actggcgctg gtttatgacg gcaccgtgat
     4561 cccggggcag tttttaagct gggaagggac gctcactgcc gttgacggcc ctgcatcggg
     4621 gatgacttcc gtcgatatcg gcgttgcaga agcgggtgaa ctggaaggaa aatcgctgca
     4681 actctccggg gaatccaacg aatatgacgg tttcacctgg caaatgccat ctgaagaaac
     4741 aatgggagat gtgaacaatg atcagttctt tcttcatttc ggcctgaagg agccgggttt
     4801 gagccacgtt gattttttcc cgaatcccgg aaacggtgaa ggacaaatct tcaatccttt
     4861 ctccaataat ctggtcttaa atgtgtttag tgtagatgga aggatgataa ggcaaataac
     4921 ggttaaaccc ggagaaaatc tcattcgcct ggaaaacctt gcctccggaa tctacctgat
     4981 caatttacag gaggaaaacg gaagacccat cggtaacgat aagctggtaa tccggtagcc
     5041 tttagatttt cagtacatat tggagggcaa tggtccgggg tggttgaata tcacccggcc
     5101 tgcccatgta ttccttatta aatatattct tgattatcag gctgatttca gatgatgcgg
     5161 tgagttgata cccggtgcgg atatcgatca ccacagctcc tttattattt gcttcgcgat
     5221 aatctttcag gccgggaaag aactcctggc caaggataac cgtttcgaaa gcctcgtcga
     5281 tccgttccgt aaagctctgg taattgatcg taattcccag gttataatgc ctgtactcca
     5341 cctccgcatt gcctttggcc gaatgcctgt aacggtattt cagtatctca ttttctatgg
     5401 aatcaaccga atggtcaatc ggatcaagat aggtgtagcc ggcaaaatag ctgaggagaa
     5461 aagccccggt tttcacctgt ccgttcagtg agatatcgat ccccgcaatg cgggccacac
     5521 cgatattgac cgatttgaac ccgatatggt ccagggttgg aagggtattg ctgtcgggca
     5581 ggtacaaccc gaaggtaaat tccatcatat cctggtattc ggtccagaaa ccggccacat
     5641 ccagcgaccc gctcaagctg ccaagctgaa aaccctgccg gatgccgatt tcagcgctcc
     5701 agcccgtttc ggatgtaagg tcgggattgg ggaaaatatt caggcttccc aggctggtgg
     5761 ccgtgtactt ctctgccatg gatggaaacc ggtatccctg gccgaaggag gccctgagga
     5821 aagttgaccc ggctgccttg tagctaatcc cggcccttac cacaggcttt gcttccgcct
     5881 tttccgcgtc gagcctgttg cgctcccatc gcagccccag gctggccgac aacctgtcga
     5941 agaacctttt atcgaactgg gcaaaggcag caaaagatga acctgtatgg tttccgtaaa
     6001 gattggatct tccgtcggtg taggaagcag ccaggccggc cgccaggtgg atattgtacc
     6061 ggaactgttt ctggtactgg tactcaccga agtagtatcc ggagccattg tccttgtctg
     6121 gatcctcatc aaaagtattc tgcaccttgt aataccttgt ccgtaatgca tgttctcctt
     6181 ttttatcatc gaaataaaca acatacggat caacgttgaa acgaataccg ttcgtagggg
     6241 aaacggaaac gggattctgg acgaacgcgc ctgaatcggc gtcggtccac agcaggaaat
     6301 cggacgactc ctgccactgg atgctggccc tcagccctgc actcaatcct ttcaccccgg
     6361 gcggattata ccggataccg gccgatgccc tgccgtagta ctggtaattg tcggtccggt
     6421 agccctggtc accgaaacca ttcagcccga aggtaaggtc gaccgggccg gctttccgca
     6481 tatggcttgc tttgacgctg ctgtaaagcg ggacgccatc ccaccaccag ctcaactctt
     6541 tccgggcggg gcgtgtatag actccctgcg acaggctgac tgtcgtttcc ggtttgtcgc
     6601 ccggggcagc cgtcctcaca ttgatcactc cgttcagggc agaagagccg tatagagccg
     6661 acgaagcccc tttgatcacc tccacctggc caatgttctc aaccgggata aaactccatt
     6721 tcacctcgtt cacatcgccg gtcagcatcg gcagttcatc gaccaggatc atgacgcggc
     6781 tgccggcgcc gtaggaatag ccgctgcctc cgcggatatt ggcctgcccg tcgatcacat
     6841 ccaccccggg tataaggcga agcgcttcgt cgagctggtt agcattttgt ttctcgataa
     6901 aagtgggctt gatgatctcc atggagaccg taacatccga aaggcgctgc tcatagcggg
     6961 aagcggaaat cactgccgta ttgagcagaa cggccgattg agatagctga atgtccttgg
     7021 tcagaatttc attctccttc agagtaactg acacacggta aggctcgtaa ccgacaaacc
     7081 ggtaattcag cacatagctg cccggtttaa ctttaaggga atattggccc tgcgcatcgg
     7141 aagcggcgcc gttgaggctg tcgagaacga tattgacgcc ggccagaggg ttgctgtcgg
     7201 cggcatcagt tatgcgcccc cggatttcgg catcctgggc gtttatgttg gcctgaaatg
     7261 ccgtaacagc aaacagcaaa gccatcccct gcctcagaaa ttgatggatt gcccggatca
     7321 ttcagtgttc cgattgatgg attaacggcc gcttttgacc ataatgccgc cggtcaaccc
     7381 gattccgtct ctgaccaggt aaaagtaaga gcccttcggg agagtaaatc cattgtagat
     7441 cagctcattc tccccgcgat tcacaggata cgctgcggtg tggacgcgtt gtccggccag
     7501 ggtaaaaact tcaaaggtcg cctcccctgt atggcttgtt gagtaaggga taacggtcat
     7561 gtcaacaaag gggttgggga aattctgccc ggcaaatatt tccttacttt catgaatgcc
     7621 cgaatcatcg actaccagga tagagagcga agtggagtcg gtgacgtttg cgacctcgat
     7681 ggggggccat ccggggaaag gaatgaccga aacgccaact ttaatggcga gtgggtagat
     7741 cccggcggct tcgggctggc cttccataac aacgcagtta aattcgcccg ccatgaacac
     7801 gctgtcgggg ctgtcggatt gccatgtcag gcccgggggg aggttgccga cctctttcag
     7861 cttcacatga tggatggtga tgacagtgct gtcgggcggc tggtaataga ccgccggtgt
     7921 tttaatggta gccacctggc tgtagaactg gttcaggtag gctacttcga gggaatcggg
     7981 gcatacctgg ccgttttctt ccggatcggg gcactcctca gcggtcattg gttcgcactg
     8041 ggagcggccg gagaggggga
//
LOCUS       DAOPNH010000154         8057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_9959_length_8057_cov_5.900025, whole genome shotgun sequence.
ACCESSION   DAOPNH010000154 DAOPNH010000000
VERSION     DAOPNH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9959_length_8057_cov_5.900025"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKZ74_08975"
     CDS             complement(<1..342)
                     /locus_tag="PKZ74_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92931.1"
                     /translation="MKANDFLCPYCRGHLMANTKVILAARKASGTRGLILFNPQLGEY
                     DVIVHHTFRLAEGEHIDMLCPLCHANLTDQTIHKNLARIKMIDSNGNEYDIYFSEIFG
                     EKCTYKISSDKK"
     gene            complement(572..1321)
                     /locus_tag="PKZ74_08980"
     CDS             complement(572..1321)
                     /locus_tag="PKZ74_08980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPM92932.1"
                     /translation="MKVLIIFAHPNPLSFTRAILDHFVKGLKEAGHQYEVVDLYKIRF
                     NPVFQDMDYSSFVDKDIPKDIFQQMDIRKMVIELAGGPLKRIIARMYIKNKTDDDLIK
                     LINSQIPKDVQKQQKKVAEADVLVFITQVFWMHFPALVKGWMERVLTYGFAYKLNENG
                     WKGDPDGRIPLLKIKKAIIIHPTFFNEQVYREKGFKVSMEKSIDDWSLKYPGVEKVDH
                     IYFHSILAVSDETRKKYLEIAYLKGKNLDLD"
     gene            complement(1330..1869)
                     /locus_tag="PKZ74_08985"
     CDS             complement(1330..1869)
                     /locus_tag="PKZ74_08985"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPM92933.1"
                     /translation="MIFSLVFFVVIRGILNKIPIIGKPPVKTVFFITAKMMVVVNLTF
                     LVMAGLNIKLNALFDPVFALRLIALVLLVTGTAILYVTTIQLNKDLIFGLPDSEKGRL
                     QTKGLFAFSRHPFYLGFILILFSSCLLYPNPLNITAFLTAWIIHHFIMIEEEKFLETV
                     YGEEYRIYRQKVGRYLTIK"
     gene            complement(1989..2771)
                     /locus_tag="PKZ74_08990"
     CDS             complement(1989..2771)
                     /locus_tag="PKZ74_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPM92934.1"
                     /translation="MFKVFKYSFFDLVRSRWIYAYLLFFLLVTTGLVWSGSDLSRALI
                     SLMNILLILCPLIGTLFGALYYYNSREFIDLLLALPLPRRSIFLGKYLGLAISLSASF
                     LLGIGLPMVVFGVFSSGLLTNFLTLLATGIFLTFIFTAIAFVVALKSENPIRGFGMAI
                     FIWLFLSVIYDGILLVVMMYFESYPLDRFALIASLFNPVDLSRILIMLQLDISALMGY
                     TGAVFRKFLGTGTGIAVAYSALACWIVVPVLLFIRCAGRKDF"
     gene            complement(2764..3471)
                     /locus_tag="PKZ74_08995"
     CDS             complement(2764..3471)
                     /locus_tag="PKZ74_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92935.1"
                     /translation="MKIIDLHKAYGRFGVLKGVDLELKDRAITAVLGPNGSGKTTLIK
                     CLLGMVIGQKGDILYHGEPVGRGWAYRSDIGYLPQIARFPENLKVKELVAMIRDIRNQ
                     PAETDALVALFGLDAFMNKPLRQLSGGTRQKVNILIAFMFDSPVYILDEPTVGLDPVA
                     LIRFKELLLAEKRKGKAILMTTHIVHLVEELADEVVFLLEGKVYFKGDVKALKELNKS
                     ESLEEAIAKILELEKHV"
     gene            complement(3468..4661)
                     /locus_tag="PKZ74_09000"
     CDS             complement(3468..4661)
                     /locus_tag="PKZ74_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HPM92936.1"
                     /translation="MPVFSDTIYVSEDHVPTISEALSMSEPGDVIIVQPGLYREGNII
                     INKKITLTGIKYPVADGSDSNEVFTIMSDSVVISGFQVQNCGVSYVKDRAGIRIDQQK
                     GCVIENNRLINTFFGIYLKNASDCLILNNYIEGVAQDEFSSGNAIHLWYSKNITVEGN
                     TCLQHRDGIYLEFVENSMIRKNHSERNLRYGLHFMFSNNDSYVENTFRDNGAGVAVMF
                     SHHITMQNNHFLKNWGSSAYGLLLKDISDGEMTGNVFRENTVGIYGDGANRIRMEGNE
                     FISNGYALKILGSCSGNTITGNNFISNTFDVFTNTSGNTNTFESNFWSDYTGYDLDRD
                     NKGDVPYRPVKLFSYITGRIPASMILLRSPFVDLVNFAEKVMPSITPPTLEDASPLMK
                     MIILN"
     assembly_gap    4778..4877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4878..5287)
                     /locus_tag="PKZ74_09005"
     CDS             complement(<4878..5287)
                     /locus_tag="PKZ74_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HPM92937.1"
                     /translation="MKKLFYIIPLMLFTISCTIKPEPIHYGEDNCILCQMTIMDHRYG
                     TEMVTSKGKIFKFDSIECMVDYFKETSKKGEEYRYILFTPFDQPGKLTDALTGYVLHS
                     KNLPSPMGMYLTAFEDKAAAMRFQQEKGGRIYDW"
     gene            complement(5289..5861)
                     /locus_tag="PKZ74_09010"
     CDS             complement(5289..5861)
                     /locus_tag="PKZ74_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92938.1"
                     /translation="MKNLPKYLMILAALLLVFLFFFPIWKITLFAPQYPGGISMYIWI
                     NQITGDTPGTLQNINILNHYVGMQMIEPDSIPELKYFQFIIAGMGVMALLFAWLNKRP
                     LILAWVILFIVLGAAGIYDFYLWEYDYGHNLSPTAPIKVPGMAYQPPLFGKKELLNFI
                     AYSYPHWGSLFFGLSILSGTLAFWFHKKKN"
     gene            complement(5967..7925)
                     /gene="nosZ"
                     /locus_tag="PKZ74_09015"
     CDS             complement(5967..7925)
                     /gene="nosZ"
                     /locus_tag="PKZ74_09015"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HPM92939.1"
                     /translation="MKQKLTLFSILFGLGMLFACTPKQPGTTGAGNAADAASRVYVAP
                     GQYDEFYAFLSGGFSGQLSVYGLPSGRLLKVIPVFSVDAEKGYGFNEETKPLLNSSFG
                     FLPWDDAHHPELSQTNGVPDGKYIFINGNNTPRIAKIDLSTFETTEIIEIPNSGGNHS
                     SPFTTENTEYVVAGTRFSVPFPQKDVPIDSYKEHFKGALSFISVSPEGHMDLAFQVLL
                     PAFNYDLAHSGKGASHGWTFFTSYNAEQSNTLLEANASQFDKDFIAAVNWKKAEEYVK
                     AGKAKEMPASYFHNVYDENSHMATSTEKKTVKVLIPSECPDMVYFLPTPKSPHGVDVD
                     PTGEYIVGAGKLSADMAVHSFSRMLKAIENKAFETMIDGIPVLKYEEVLGGMVSSGGL
                     GPLHTEFDGKGNAYTTFFISSEVVKWKVGSWEVLDRVPCYYSVGHLMIPGGDSKQPFG
                     KYMLALNKITKDRYLPTGPELNQSAQLYDISGEKMQLLLDFPTVGEPHYAQAIPADLV
                     KPRSKQIYKLGENTHPHAARSEADARVVREGNVVRIYMTAIRSHFAPDNIEGIQVGDK
                     VYFHVTNLEQDWDVPHGFAVMGARNAELLIMPGQTETLFWEPDRPGVFPMYCTDFCSA
                     LHQEMQGYVRVSAKGANIPLKWWVGSQQ"
BASE COUNT         2071 a         1909 c         1933 g         2044 t
ORIGIN      
        1 ttttttatcg ctgctgatct tataggtgca tttctcgccg aagatctccg aaaaataaat
       61 atcgtattcg ttcccgttgc tgtcgatcat cttgatgcgg gccaggtttt tatggatcgt
      121 ctggtcggtc aggttggcat ggcagagggg gcaaagcata tcgatgtgtt ctccttcagc
      181 cagcctgaag gtatggtgga cgatcacatc atattcgccc aactgtgggt tgaagaggat
      241 caggccgcgt gtgcccgacg ctttgcgggc agccaggatc actttggtat tggccatcag
      301 gtggccacgg caatagggac aaagaaaatc gtttgctttc atggcgattg gaaataaagg
      361 gcaaagatag ttcaaagatt ggttcgactg ttcaattgtt cgattgttac agggttacgg
      421 gttaaggggg tccctcaccg cctccggcgg ttcgggatga cgtttgagat gaaagaaaaa
      481 gtcgcgccag cgacgccagt atggtagccc ccgattaaat cgggggtaac gtacggcaaa
      541 ttccattcgg agtgctgtag gcacggcaga attaatccag atcgagattt ttgcctttta
      601 ggtaagcaat ttccaggtat ttcttcctag tctcatcgct taccgcaagg atcgagtgga
      661 aatagatatg gtcgaccttt tcaactccag gatatttcag gctccaatca tcgattgatt
      721 tttccatgga aactttaaat cctttctccc tgtatacctg ttcattaaaa aaagtaggat
      781 ggatgatgat ggcttttttg attttcagaa gaggtatcct tccgtcaggg tcacctttcc
      841 acccattctc atttaattta taagcaaatc catacgtcag gaccctttcc atccaacctt
      901 ttaccaacgc cgggaagtgc atccagaata cttgcgtgat aaagaccaga acatcggctt
      961 ctgcaacttt cttttgctgt ttctggacat ctttcggtat ctgcgagttg atcagcttta
     1021 tcaggtcatc atcggtctta ttcttaatat acatccgggc aattatcctt tttaacggcc
     1081 cgccggcaag ttcaatgacc atcttcctga tatccatctg ctgaaagatg tctttaggta
     1141 tatctttatc aacaaaagag gaataatcca tatcctggaa aacaggattg aatctgatct
     1201 tgtacaaatc gaccacctca tactggtgac cggcttcctt caggcctttg acgaaatggt
     1261 cgagtattgc cctggtaaac gacaatggat ttggatgagc aaaaatgatc agtactttca
     1321 tggttcattt catttaattg tcaggtatct tccaactttt tgcctgtata tcctgtattc
     1381 ctccccataa actgtttcaa gaaacttttc ctcttctatc ataataaaat gatggatgat
     1441 ccaggccgtg agaaaagcgg taatattcaa cggattggga taaagaaggc aggaggagaa
     1501 gagtatcagg ataaagccaa gataaaaagg atgacggctg aaagcaaaaa ggcctttggt
     1561 ttgcaggcga cccttttcag aatcaggcaa accgaagatc aggtctttgt tgagttgaat
     1621 ggtcgtcaca tatagaatag cagtgcccgt gaccagcaga acaagggcga tcagcctgag
     1681 ggcaaataca ggatcaaata atgcattgag ctttatattc aatccggcca ttaccaagaa
     1741 tgtgaggttg accactacca tcattttggc ggtaatgaag aagactgttt taacgggagg
     1801 tttgcctatg atgggtattt tattcaggat accgcggata acaacgaaaa agacaagtga
     1861 aaaaatcaaa aaaacgatta gaagaaccag gaaatttaac atgggaggcg aatttacccc
     1921 caaatataac atttatattg aataaatatc gattcttttt attaaaaggg gacgaggtag
     1981 cgaacggact aaaagtcttt cctccctgca caccggataa acagtaatac cggtaccact
     2041 atccagcatg ccagtgccga ataagccacg gcaatcccgg ttccggtccc caaaaatttg
     2101 cggaaaacgg cgccggtgta tcccatcagc gccgaaatat cgagctgcag catgatcagg
     2161 atgcgcgaca ggtcgacggg attaaacagg gaggcgatca atgcaaaccg gtcgagcggg
     2221 taactctcga aatacatcat gacaaccagc aggataccgt cgtagatgac cgagaggaac
     2281 agccagataa aaatcgccat tccgaagccg cggattggat tttccgactt cagcgccacc
     2341 acgaaggcga tggcggtaaa aataaacgtg aggaaaattc ccgttgccag gagggtcagg
     2401 aaattcgtca acagcccgga tgaaaacacg ccgaaaacga ccatgggcag gccgatcccc
     2461 agtaaaaagc tggctgaaag cgaaatggcc agccccaggt atttgcccag gaaaatcgaa
     2521 cggcgcggca gcggcaaagc cagcaggaga tcgataaatt cccttgagtt gtagtagtat
     2581 agcgctccga aaagggttcc gatcagcggg cagaggatca gcaggatgtt catcaggctg
     2641 atcaaggccc tggacagatc gcttcccgac cagaccagcc cggtggtcac cagcaggaaa
     2701 aacagcaaat atgcgtaaat ccagcggctc cgtaccaggt cgaaaaagct gtatttgaat
     2761 accttaaaca tgcttttcca gttccagtat ttttgcgatg gcctcttcca ggctctccga
     2821 tttgttcagc tctttaagag ccttcacatc gcctttgaaa tagaccttcc cttcgagcag
     2881 gaacactacc tcgtcggcca gctcctccac caggtgtaca atatgcgtgg tcatcaggat
     2941 cgctttgcct ttccgtttct ctgccagcag caactccttg aaacggatca gcgccacggg
     3001 atccaggcca accgtaggct cgtcaaggat ataaacggga gaatcgaaca tgaaggcgat
     3061 gaggatgttg accttctgcc gcgtgccgcc cgacaattga cgcaaaggct tgttcataaa
     3121 tgcgtccagg ccgaacaggg ccaccagggc atcggtttcg gcaggctggt ttcggatatc
     3181 gcggatcata gccacgagtt ccttcacttt aaggttctcc ggaaaacggg caatctgcgg
     3241 caaatatcct atatccgagc gataagccca gccgcggcca acaggttcgc catgatacag
     3301 aatatccccc ttctgcccta tgaccatccc cagtaaacat tttatcagcg tggttttccc
     3361 cgatccgtta gggcccagca ctgccgttat tgctctgtcc ttcagctcca ggtctacccc
     3421 tttcagcacg ccgaacctgc cgtaggcctt atgtaaatct atgatcttca attcagtatg
     3481 atcattttca tgagaggcga agcatcttcc agcgttggag gtgtaatcga tggcattacc
     3541 ttttcggcaa agtttaccag gtcgacaaac gggctgcgca gcaggatcat cgaagcggga
     3601 atccggccgg tgatatagga gaaaagcttc accggtctgt agggcacatc gcctttgttg
     3661 tcgcggtcga ggtcatatcc ggtgtaatcg ctccagaaat tggattcgaa tgtattggta
     3721 ttgccggagg tattggtaaa aacatcaaag gtattggaaa tgaaattgtt cccggtgatc
     3781 gtattgccgg aacaactgcc caggatcttg agggcatagc cgttggaaat aaactcattg
     3841 ccctccattc ttatccggtt ggcaccgtcg ccgtaaatcc cgacggtatt ctcccggaat
     3901 acattccctg tcatctcccc gtcggaaata tcttttaata ataacccata agccgaactg
     3961 ccccagttct ttagaaaatg attattttgc atggtaatgt ggtgcgagaa catgaccgca
     4021 acgcctgcgc cgttatcccg gaaagtattc tccacataac tgtcgttgtt ggaaaacata
     4081 aaatgaagac cgtaccggag gtttcgctca ctgtgattct tcctgatcat gctgttctcc
     4141 acgaattcaa ggtaaattcc gtcacggtgc tgaaggcagg tattcccttc cacggtaatg
     4201 ttttttgaat accataaatg aatggcattg cccgatgaaa attcatcctg tgcaacgcct
     4261 tcaatataat tgttcaggat caggcaatca ctggcatttt tgagataaat gccaaaaaat
     4321 gtattgatca ggcgattgtt ttcgatgaca catccctttt gctgatcgat tctgatcccg
     4381 gccctgtctt tcacatagct caccccgcag ttctgcacct ggaatccgct aattacaacg
     4441 ctgtcggaca taatggtgaa cacttcattc gaatccgagc catcggccac agggtacttt
     4501 atccccgtaa gggtaatttt tttatttatg atgatattcc cttcccggta taaccccggc
     4561 tgaacgatga tgacatcacc tggttcagac atagataatg cttctgagat cgtgggaaca
     4621 tgatcttcag aaacataaat ggtatccgaa aataccggca taccaatgat aatcaagaga
     4681 taagcaaaag ttattctact tggcctcaca caaagtccca caaaggtttt acaaagaatc
     4741 aaaatatcaa taaatcaaaa gttcaaaagt tcaaaagnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnttc cagtcatata ttctcccgcc tttttcctgt tgaaaccgca
     4921 tagccgccgc tttatcctca aaagccgtaa ggtacattcc catcgggctg ggcaggtttt
     4981 tgctgtgcag cacataaccg gtgagggcat cggttaattt acccggctga tcaaaaggag
     5041 taaaaaggat ataacggtat tcttcacctt ttttcgacgt ttccttaaag taatccacca
     5101 tacattctat ggaatcgaat ttgaagattt ttccttttga cgtaaccatc tcagtcccgt
     5161 acctgtggtc cataatggtc atctggcaaa ggatgcaatt gtcttcaccg taatgaatag
     5221 gctccggttt aatggtgcaa gagatggtaa agagcatcaa agggattata taaaacagct
     5281 tcttcatttt aattcttctt tttatgaaac cagaaagcaa gggtaccgga tagaatggat
     5341 aatccgaaga acaggctgcc ccagtgcggg taggaatagg caataaagtt aagcagttct
     5401 ttcttcccga acagcggggg ctggtaggcc atccccggca ccttgatcgg ggcggtggga
     5461 gaaaggttat gaccatagtc gtattcccag aggtagaagt cgtagattcc ggcagcaccc
     5521 aatacaatga aaaggataac ccacgccagg atcagcgggc gtttgttcag ccaggcaaac
     5581 aaaagggcca tgactcccat tcctgcaatg atgaactgga agtacttcag ttcggggatg
     5641 gaatcgggtt cgatcatctg cattccgacg taatgattca ggatattgat gttttgcagt
     5701 gtcccggggg tatcgccggt tatctggttg atccagatgt acatcgagat accgccgggg
     5761 tactgcggag cgaacagcgt gatcttccag atcgggaaaa agaagaggaa gaccagcaga
     5821 agcgctgcaa ggatcatcag gtatttcgga agatttttca tgtgttgata ttgatttttg
     5881 ctccgacgac tgaagccgtc ggttccatta ttgttttact ttggaaccaa cggctttagt
     5941 cgttggacta cctcacattt ggttgattat tgctgagagc cgacccacca tttcaacgga
     6001 atgttggctc ctttagcgga tacccgcaca taaccctgca tctcctggtg cagcgccgag
     6061 caaaagtcgg tgcagtacat cggaaaaacg cccgggcggt cgggctccca gaagagcgtt
     6121 tcggtctgtc ccggcatgat cagcaactct gcattacggg cgcccatgac ggcaaagcca
     6181 tgaggcacgt cccagtcctg ctccaggttg gtcacgtgga aatagacctt gtcgcccacc
     6241 tggatgcctt cgatgttatc cggagcgaaa tggctgcgga tggcggtcat atatatgcga
     6301 accacattgc cttcacgcac caccctggca tccgcttcgc tacgggcagc gtgcggatgg
     6361 gtattttctc ctagcttata gatctgcttt gagcgtggct ttaccaggtc ggcagggata
     6421 gcctgggcgt agtgaggttc tccaacggtc gggaaatcga gcaaaagctg catcttttcg
     6481 cctgagatat cgtaaagctg cgccgactgg ttcagctccg gaccggtagg caggtagcgg
     6541 tctttcgtga tcttgttcag ggccagcatg tatttcccga aaggctgctt cgagtcgccg
     6601 ccggggatca tcaggtgacc gaccgaataa tagcagggta cccggtcgag cacctcccat
     6661 gagccgacct tccacttcac cacttccgat gaaatgaaga atgtcgtata ggcatttcct
     6721 ttgccgtcga actccgtgtg cagcggtccg aggccgccgc tggagaccat cccgccaagg
     6781 acttcttcat actttagcac cggaatccca tcgatcatcg tctcaaatgc cttgttttct
     6841 attgctttca gcattctgga aaacgaatgg actgccatgt cggccgataa tttgcctgcg
     6901 ccaacaatgt attcgcctgt cggatcgaca tcaaccccgt gaggtgattt cggggtcggg
     6961 aggaaataga ccatatcggg gcattccgac gggatcagca ccttcaccgt tttcttctcc
     7021 gtagaggtgg ccatgtggct gttttcatca tacacattat ggaaatagct ggccggcatc
     7081 tcttttgctt tgccggcttt tacgtattcc tccgcttttt tccagtttac cgcggcgatg
     7141 aaatccttgt cgaattgcga cgcgttggct tcgagcaatg tattcgactg ttcggcatta
     7201 tagcttgtga agaaagtcca gccatgcgaa gcgcccttgc cggaatgcgc gagatcgtag
     7261 ttaaatgccg gcagcagcac ctggaaagcc aaatccatat gtccttccgg gctcacggaa
     7321 atgaacgata gcgcaccttt gaaatgctcc ttgtaggaat cgataggcac gtctttttgc
     7381 gggaaaggca cactgaagcg ggtgcccgca actacatatt ccgtgttttc agtggtaaat
     7441 ggagaactat ggttcccccc gctgttcggg atctcgatga tctccgtcgt ttcgaaagtg
     7501 gacagatcga tttttgcgat cctgggtgtg ttattcccat tgatgaagat gtacttacca
     7561 tcgggaacgc cattggtctg cgacagttcc gggtggtgtg cgtcgtccca tggcaggaat
     7621 ccgaacgagg aattgagaag cggcttggtc tcttcgttga aaccatagcc tttttcggca
     7681 tcgaccgaaa aaaccgggat cactttcagc agtcttcccg aagggaggcc ataaacgcta
     7741 agctggcctg aaaacccacc ggaaaggaat gcatagaatt cgtcgtattg cccgggtgca
     7801 acatacaccc tggaggctgc atcggcagca ttaccggctc ctgtcgttcc gggttgcttc
     7861 ggcgtacagg caaaaagcat acccaggccg aagaggatcg aaaatagtgt tagtttctgt
     7921 ttcataggtt attgggttga gagatttgtc ataaggtggt cataaggtgg tcataaagtg
     7981 gtcgtgatac ttacagccga ctgccgactt attttatcgt ccggaaatac tccagcacag
     8041 cacgtgcttg ttcctgt
//
LOCUS       DAOPNH010000155         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10002_length_8039_cov_5.915957, whole genome shotgun sequence.
ACCESSION   DAOPNH010000155 DAOPNH010000000
VERSION     DAOPNH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_8039_cov_5.915957"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="PKZ74_09020"
     CDS             <1..2068
                     /locus_tag="PKZ74_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM92940.1"
                     /translation="IDAVVRHTAVIAVLGINIQNSNNCRVSYNSVNITNTDAQGKSKG
                     INLRNTDGVTGRNNIFYIKSSGIPIHVDQNVTNLALDYNNYYNPAGIIGKLLNQVFNN
                     LSQWGQSISGDANSLAVHPYFKSDTIPLPYQRILNGSGIPVAGIRYDIDGKLRHIQAP
                     DMGCMEFFIDFGVLEMISPNLECFHEDLEPVIVYIRQFGDVPFDNLIIRYQLNDGDIH
                     TDTIQGPLEFDFIHEFDETETLETYGDYLFKIWLVNNADDNINNDLLQVWRYSKPSPI
                     ISIDYNNACTGNEVYFTANATVDSPYYIEKYEWIFGDGDTAYTQNPVHLFDEAGSYPV
                     VCRAYSNAGCYNDTSFVITLDPAYELLSISYDLDDETCHNSGTGTLQMSAVGGTPPYT
                     FYLNGSAVPDGFIASLASGKYQLSVEDAAGCTAVDSAEARTLIFMDPEIIASQLTGNA
                     PFNVNFDYEADGATGCTWHFPGNITDTLKNATFTFTEYGDYTIVLEVNSGEPFYCIET
                     DTVNIHVDIIVVIEPNSVFTPNGDGHNDYFEVKMYGLETMNVKIFNQWGNRIYEITDV
                     EGKWDGNTDDGVEAADGTYFYNLTARGVDGLEYSRQGTVMLLRHGAVAFPNPVLNSVS
                     IKTFGPLDPPVYIEIFSVFGEKVYSGLETQPGNIHLDLAELSKGIYFIRVHDNRKEYY
                     VRIIKN"
     gene            2075..4513
                     /locus_tag="PKZ74_09025"
     CDS             2075..4513
                     /locus_tag="PKZ74_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM92941.1"
                     /translation="MINIRYIFFMMMAVLVTASTGYAQKKEQYIAVSLFRDLQFNIAA
                     ENYDKALRKMKGITPEKLHCTFNLAECYRLMNDPDLAEPYYRQLAETSYGDTVPVYYL
                     RYATILKTRGDAESARKYFEKYLQTDPGNMEAETGLKSCDWLLENRHKKAQVIVEEIT
                     RINTPDDEFGPAYLNPQKNSIIFTSNRVTEDSKNIDQWTGSRFSDLYISAFDGKNWSD
                     PELAEYGGLLNSDVHEGTPSLNGDYSVMYYTRCGKTANEKSFCEIWKTVKTGDQWSKP
                     APVLTDSVANIGHPAISKDERILIFSSNRPGGTGGKDLWAAKRNSADEPFGRPELLGP
                     GINTAGDELFPYLYNDTTLLFASNGYEGYGGLDIFISNLRKNKWTKPENLLAPINSSY
                     DDFGIILKKPFEEGCFCSNRNGGTGGDDIYYFYRRILLFTASGQVKDNSTLLSVKGAE
                     VILAGKAGDTVKVMTDKQGLFRFDNSQVKEDVDYELVFRKQGYFSKKETFSTRPYEED
                     HDFSIDIKIDPIPEKPIVLPDILYELDKWDLAPQYQDSLLQLVRLLKDNETLVIELRS
                     HTDSRGSAEYNNVLSQKRAQSVVDFIVSQGIDPGRLVAKGYGKRIFRMLDKDIVKDGY
                     TFKAGTELNDDFVNRLPSKEAREAAYQLNRRTEFAVIAKDYKPGSSAGSTAVIEVISD
                     TSLAALDYNLTPEGKMAVEVYINDYSAGALIDRTINSSLIDEKIVLDLLQKGAIDRND
                     FQGVFEQILIDDRITENTVILLRKIRLGGIMVGDHPVKVISDAGNKLIIGNDLLEKAG
                     SFIIDESNKKLIFK"
     gene            4518..5465
                     /locus_tag="PKZ74_09030"
     CDS             4518..5465
                     /locus_tag="PKZ74_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPM92942.1"
                     /translation="MKKPSVIFLFILLAGSLFSQQEPQYNQHMFTRAIINPAAAGISQ
                     AVDATLFGRHQWIGYKDNDSSPVYPKLYGISLDIPIYAIHSGVGLNLRHYTAGAEKSL
                     DIRLQYAYHFTLNKRNTLSFGMSFNLLNKTIDYSQLLPAEFDPLLESSKAESGLVTDI
                     GLGIHYRYGDKFNAGISSASLLGNSAGIGGPDFKLERHYYGYGSYDIEIQWSGEALVL
                     TPGFLVKTARGAFNADIYALVTYNDFIWGGLMYRVESAAAILAGISYNGIKAGVSYDY
                     TMSKGFAAGSRHSVELFVKYSYPISPKVVKRSGYNTRNL"
     gene            5723..5794
                     /locus_tag="PKZ74_09035"
     tRNA            5723..5794
                     /locus_tag="PKZ74_09035"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5755..5757,aa:Met,seq:cat)
     gene            complement(5958..>8039)
                     /locus_tag="PKZ74_09040"
     CDS             complement(5958..>8039)
                     /locus_tag="PKZ74_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92943.1"
                     /translation="VSTISEKVETSTALIVTSRDAIPWDGVPICCIFTCSYGIKPPNQ
                     KKMRVVNVLLCSALIVFSPSVLGQGPLSLDSTCRIIETSMVITPTTCNGASGSITGLT
                     FLGSPPFTFQWLNGAGDPYGSSIDAFGLPAGQYRLTAADGNGCQKNSPYYSIVDAGNL
                     QVTGVQITNPHCDRPDGQIIVQAFSPSGSTLEYSIDDGVSYQADGVFSGLAGGVYVVR
                     VKDVNGCYGFYPFNPIVLADIPGPQVQQIVVTDETDFLSNGSMEIIATGSTATLYYSI
                     DNGNTYQSNNGTFNNLTAGIYICIVKDENDCDTTFMIEIQNIILTYIHIVTGEGGHCL
                     GSTAMVPLDVNNFQSVASFHLRLSYNADKLQCEGFANVHPQLADSLSGLVDPVAGDIH
                     LAWNSSTPVTFIQPETLAELVFTTINPGQGDIQWYTGDTDSYFLDSEGNPVPADFQNG
                     EVMIYEPPEIMLDQSKTVCAGQFVSIMSIATGNHPPFSYRWIYPTGDSTSSDPFFVSV
                     TPADAGLYTLLATDQVGCTDQEIIELIVEEIPIAAFHGNDTLELHTGDMLDAGAGLYS
                     CLWNTGDTTAGIVINAAGMYIVEMESLWGCFGVDSVYVKLTYEEITESNVPAILLYPN
                     PAKDILTIQFEEMKDNAGVIIYDIRGKMMVSKRIQQEITTIDAGNLDPGAYFVEISNN
                     HFSIVRKLMVH"
BASE COUNT         2138 a         1871 c         2022 g         2008 t
ORIGIN      
        1 tattgacgct gttgtaagac acacggcagt tattgctgtt ctgggtatca atatccagaa
       61 cagcaataac tgccgtgtgt cttacaacag cgtcaatatt accaataccg atgcgcaggg
      121 taaaagtaaa gggataaacc tgagaaacac cgacggcgtg acgggcagga ataatatctt
      181 ttacataaaa tcctccggga ttccgattca tgtcgaccag aatgttacca acctggcgct
      241 cgactataat aactattaca atccggccgg aatcatcgga aagctgttga accaggtatt
      301 caataacctt tcgcaatggg ggcaaagcat ttcaggagat gccaattcac tggcggtgca
      361 tccctatttc aaatccgata ccattcctct gccctatcag cggattttga acggttcggg
      421 cattccggta gcggggatca ggtatgatat cgacggcaag ctgaggcaca ttcaggcgcc
      481 tgacatgggt tgtatggagt ttttcataga tttcggtgtg ctggagatga tcagccccaa
      541 cctggagtgc ttccatgagg acctggaacc ggtcattgta tatatccgcc agttcggcga
      601 cgtaccgttc gataacctca tcatcaggta ccagttgaat gatggcgaca tccacaccga
      661 tacgatccag gggccactgg aattcgattt tatccatgag ttcgacgaaa ccgaaacttt
      721 ggaaacttac ggggactacc tttttaaaat ttggctggtg aacaatgcag atgataatat
      781 caacaacgat ttgcttcagg tatggcgcta ttcaaagcca tctcccatta tatcaataga
      841 ttataacaat gcctgtacag gtaatgaagt ctactttacc gcgaatgcaa ctgttgattc
      901 cccctattat atagaaaagt atgaatggat tttcggggat ggtgatactg catacaccca
      961 gaacccggtc cacctgtttg atgaagccgg atcgtatccg gttgtatgcc gggcttacag
     1021 caatgccggt tgctacaacg atacgagttt tgtcatcact cttgatcctg cgtatgaact
     1081 tctctccatt tcgtatgacc tggatgatga gacctgtcat aacagcggca ctggcaccct
     1141 tcagatgtcg gcggtgggag gcacgccgcc ttacaccttc tacctgaatg gcagcgcggt
     1201 tccggatggg tttatcgcca gcctggcttc gggcaaatat cagctgagtg tggaagatgc
     1261 tgccggttgt acagccgtgg attcagcgga agcccgtacc ctgatcttca tggatcctga
     1321 aatcattgcc agtcagctga caggtaatgc ccctttcaac gtcaactttg attatgaagc
     1381 tgacggggca acaggctgta cctggcattt tcccggaaat atcacggata ccctcaaaaa
     1441 cgcaactttc acttttactg aatacggcga ttacacgatc gtgctggagg ttaattcggg
     1501 tgaacctttc tattgcattg aaactgatac ggtgaatatt catgtcgata ttattgtcgt
     1561 catcgagccc aattcggtat ttacacctaa cggggatggg cataatgact attttgaagt
     1621 gaagatgtat ggtttggaga cgatgaatgt aaaaatcttc aaccaatggg gaaacaggat
     1681 ttatgagatc acggatgtgg aaggcaaatg ggacggtaat accgatgacg gggttgaagc
     1741 ggccgatggc acttatttct ataacctgac tgcccgtgga gtggacggcc tggaatattc
     1801 gcgccaggga accgtaatgc tgcttcgcca tggtgctgtg gcttttccga atcccgtgct
     1861 gaattcggtc agcattaaga cattcggccc cctggatccc ccggtttata tcgaaatttt
     1921 ctccgttttc ggagaaaaag tgtattcagg gttggaaacc caacccggga atatccatct
     1981 tgacctggcc gagctaagta aaggcattta tttcatccgg gtgcatgaca accggaaaga
     2041 gtattatgtc aggatcatta agaactaatt gtaaatgatt aatatacgat atattttctt
     2101 tatgatgatg gctgttctgg taacagctag cacaggatat gcacagaaaa aggagcagta
     2161 tattgccgtt tcccttttcc gtgaccttca gttcaatatc gctgcggaaa actacgacaa
     2221 agccctccgc aagatgaagg ggatcactcc cgaaaaactg cactgcactt ttaacctggc
     2281 cgaatgttac cgcctgatga acgatccgga cctggccgaa ccttattaca ggcagctggc
     2341 agaaacctca tacggcgata ccgtccctgt ttattatctt cggtatgcaa cgatcctgaa
     2401 aactcgtggg gatgctgaat ccgccagaaa atattttgag aaatatctgc aaaccgatcc
     2461 cgggaacatg gaagcggaaa cggggctcaa atcatgcgac tggctattgg agaaccgtca
     2521 taagaaggcc caggttatcg tggaagagat cacccggatc aatacccccg acgatgaatt
     2581 cgggccggca tatctaaatc cgcaaaagaa ttccatcatc tttacttcca accgggtaac
     2641 ggaagactca aaaaacattg accagtggac cgggtcgcga ttctcggacc tgtatatcag
     2701 cgcttttgac ggaaaaaact ggagtgatcc ggaattggcc gaatatggcg gactgttgaa
     2761 ttcggatgtc catgaaggga cgccatccct aaacggggat tattctgtga tgtattacac
     2821 ccggtgtggt aaaacggcga acgagaagtc gttctgtgaa atatggaaaa ccgtcaaaac
     2881 cggtgaccag tggagcaagc ctgcaccggt gctcacggat tcggttgcaa acatcgggca
     2941 tccggccata agtaaggatg aaaggatact tatcttctcg tcgaacagac cgggcggtac
     3001 tggcggcaaa gatttatggg cagccaaacg gaacagcgct gatgaacctt tcggccggcc
     3061 ggaattgctg ggccccggga tcaataccgc cggcgatgag ttgttccctt acctttataa
     3121 cgacacgacc ctgctgtttg cttccaatgg ttatgaaggc tacggagggc tggatatatt
     3181 tatctctaat ctcagaaaaa acaaatggac caagccggaa aaccttctgg caccaattaa
     3241 ttcttcttac gatgatttcg gaatcatcct gaaaaagcct tttgaagaag gctgtttctg
     3301 ctccaatcgc aatggcggca ccggcggcga cgatatttat tatttctaca ggaggatatt
     3361 gcttttcacg gcatcaggcc aggtgaaaga caacagcacc ctgctatccg tcaaaggtgc
     3421 agaggtgatc ctcgctggta aggccggtga tacagtcaaa gtgatgaccg ataagcaggg
     3481 cctattccgt ttcgacaatt cccaggtaaa agaagatgtg gactatgaac tggtttttcg
     3541 taaacaagga tatttttcaa agaaggaaac attctccacc cggccgtatg aagaggatca
     3601 cgatttttcg attgatataa agatcgatcc gattcctgag aaacccatcg ttctgcccga
     3661 cattctttac gaactcgaca aatgggatct tgccccgcaa taccaggatt cacttctgca
     3721 actcgtgcgc ttgctgaaag acaatgaaac gctggtgatt gagctgcgtt ctcataccga
     3781 ttctcgcggg tcggccgagt acaacaatgt gctgtcgcag aaaagggcgc agtcggtcgt
     3841 cgatttcatt gtcagccagg gtattgatcc gggacggctg gtagcgaaag gatacggtaa
     3901 gcggatattc aggatgctgg ataaagatat cgttaaggat ggttatactt tcaaagcagg
     3961 cacggagctg aatgatgatt tcgtcaaccg tctcccttcg aaagaggccc gtgaagcggc
     4021 gtatcagctt aaccggcgga ccgaatttgc cgtaattgca aaggattaca agcccggttc
     4081 ttcagcagga agtacagcgg ttatcgaagt gatatccgac acatcgctgg cggcgctgga
     4141 ttacaatcta acgccggaag gcaagatggc cgttgaggta tacatcaacg attacagcgc
     4201 cggggcactg atcgacagga ctatcaactc atcactgatc gatgaaaaga tcgtgctgga
     4261 cctcttgcag aaaggagcga tcgaccggaa tgatttccag ggggtattcg aacagatcct
     4321 tattgatgac cggattacgg aaaacacggt cattttgctc agaaagataa ggctgggcgg
     4381 gatcatggtc ggagaccatc ctgtaaaagt cataagcgat gcgggaaaca agctcatcat
     4441 tggcaacgac ctgctggaaa aggccggttc gtttattatt gacgaatcga ataaaaaact
     4501 gatattcaaa tagaaatatg aagaaaccat ccgtcatatt tcttttcatt ttgctggccg
     4561 gaagtctgtt ctcgcagcag gagccgcagt acaaccagca catgttcacc cgggcaatca
     4621 ttaacccggc agccgccggt atttcgcagg cggtcgatgc gacccttttc ggcaggcatc
     4681 aatggatcgg gtacaaggat aatgacagtt ccccggtcta cccgaagttg tacggtattt
     4741 ctctcgacat tcccatttac gccatccaca gcggcgttgg gttgaatctt cgccattata
     4801 cagccggggc ggagaagagc ctcgacatcc ggctgcaata tgcctatcat ttcactctca
     4861 ataaaaggaa caccctcagc tttgggatgt cgttcaacct gttaaacaag acgatcgact
     4921 attcgcagct cctccccgcg gagtttgatc ccctgctgga gagcagtaaa gcggagagcg
     4981 gtctggtcac cgacattggg ctcggtatcc attaccgtta cggcgataaa ttcaatgccg
     5041 gcatttcatc ggccagcctg ctgggcaatt cggccggtat cggcggtccc gatttcaaac
     5101 ttgagcgaca ctactatggg tacggcagtt acgacatcga aatccagtgg agcggagagg
     5161 ctttagtact cacgccgggt ttcctggtca aaacggccag aggggcattc aatgccgaca
     5221 tttatgccct ggtgacttat aacgacttca tctggggcgg gctgatgtac cgggtcgaga
     5281 gcgctgccgc catcctggca ggcattagtt ataacgggat aaaggcgggt gtgtcgtacg
     5341 attacacgat gagcaagggt tttgctgccg gcagccgtca cagtgtggaa ttatttgtaa
     5401 aatattccta tcctatttca ccgaaggtgg tcaagcgcag cgggtacaat acgaggaacc
     5461 tttagcaaaa ttccaagcac caaattccaa gcaccaaatt ccaagcaaat tcaaaatatc
     5521 aaatttaaaa tctaaattgt tttgaaatta tttttttgga tttgattttt gtttggaatt
     5581 tggaatttgg tgcttggaat ttattaaata tttattgtat ctttgcaccc cgtttggatt
     5641 aaattgaacg gttatattgc ggggtggatc cgccggctgg cggatcgtcg gactcaaatt
     5701 ccgaagatcc ctgaagacat attgcggggt ggagcagtgg tagctcgtcg ggctcataac
     5761 ccgaaggtcg ttggttcgaa tccagccccc gctacaaaac aaaagcccgg cctatagcaa
     5821 caggtcgggt tttttgttga accaatcggg acgccatccc ttgggatggc gtcccgattg
     5881 gttcaactgg ggttcttctt ttgacgacaa aagccgtcgg ttccaaaaag gcaggatggg
     5941 atcgactata aaattcctta atgcaccatc agcttccgga ctatactgaa atggttgttt
     6001 gatatctcaa cgaaataggc acccggatca aggtttccag cgtcaatcgt ggtaatttcc
     6061 tgctgaatcc gttttgaaac catcatcttt ccccggatat cgtatatgat cactccggca
     6121 ttatctttca tttcttcgaa ctgaatagtc agaatatcct tggcgggatt tggatagagg
     6181 aggatcgcgg gcacattgct ttcggtgatt tcttcatatg tcagcttgac atataccgaa
     6241 tcaacaccga aacaacccca aagagactcc atctcaacaa tatacattcc tgcggcattg
     6301 attacaatac ctgccgtggt gtcgccggta ttccacagac aggaataaag tccggccccg
     6361 gcatccagca tatccccggt gtgcaattcc agcgtatcat tgccatgaaa agcagctatc
     6421 ggaatctctt ctacgattag ctcgataatt tcctggtcgg tgcagccaac ttggtcggtg
     6481 gcaagcagag tatagaggcc ggcatcagcg ggggtgacgc tgacgaagaa cgggtcggag
     6541 ctggtgctat cgcccgtcgg ataaatccaa cggtaactaa agggtgggtg gttgcctgtc
     6601 gcaatactca ttatgctgac gaactgcccg gcgcagacag ttttactctg atccagcatg
     6661 atctccggtg gttcataaat catcacctcg ccattctgga aatcagccgg aacaggattg
     6721 ccttcggaat ctaagaaata gctatcggta tcacccgtgt accattgaat gtcgccctgt
     6781 cctggattaa tggtggtgaa cacaagctct gccagggttt ccggttggat gaatgttacg
     6841 ggtgtagatg aattccaggc taaatgaata tctcccgcca ccgggtcgac caatcctgag
     6901 aggctgtcgg caagttgcgg atgaacattg gcgaagcctt cgcattgcag tttgtcggcg
     6961 ttgtaactta atctcaaatg aaaggacgca acggattgga aattattgac atcaagagga
     7021 accatagccg tacttccaag gcaatgtcct ccttcgccgg taactatatg tatgtaagtc
     7081 aggatgatgt tttgaatctc aatcatgaaa gtcgtgtcgc agtcgttttc gtctttgacg
     7141 atgcagatat atatacctgc tgttaaattg ttgaaggttc cattgttcga ttggtaggtg
     7201 ttgccgttgt caatagagta atagagcgtt gctgtgcttc cggttgctat aatttccatc
     7261 gatccgtttg acaaaaaatc ggtttcatcc gtgacgacga tttgctgtac ctgcgggccg
     7321 ggaatatctg ccagaacaat cgggttgaaa ggataaaaac cataacatcc attcacatcc
     7381 ttaacccgca ccacgtaaac cccaccggcc aacccggaga aaacgccgtc ggcctggtac
     7441 gaaacgccgt catctattga atactcaaga gtggacccgg agggcgagaa cgcttggacg
     7501 ataatctggc catccggccg gtcacaatgg ggattggtta tttggacccc ggttacctgg
     7561 agattccctg catctacgat gctataataa ggagaattct tctggcagcc gttgccgtcg
     7621 gcggcggtga gccggtattg tccggctggg aggccaaacg catcaatgga tgagccataa
     7681 ggatcaccgg ccccgttaag ccactggaag gtgaaaggcg gagagccgag gaaggtaagc
     7741 ccggtaatcg aaccgctggc tccattgcag gttgtagggg tgattaccat tgaagtttcg
     7801 atgatgcggc aagtagaatc gagtgaaagg ggcccctgac caagaacaga aggggaaaaa
     7861 acaatcaggg ctgaacaaag aagcacattc acaacacgca ttttcttctg gtttgggggt
     7921 ttaataccat atgaacaggt aaatatacaa caaattggga cgccatccca agggatggcg
     7981 tcccgacttg tgacaattag tgcggtagaa gtttccacct tttcactaat ggtgctaac
//
LOCUS       DAOPNH010000156         7926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10229_length_7926_cov_5.148901, whole genome shotgun sequence.
ACCESSION   DAOPNH010000156 DAOPNH010000000
VERSION     DAOPNH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10229_length_7926_cov_5.148901"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKZ74_09045"
     CDS             complement(<1..387)
                     /locus_tag="PKZ74_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPM92944.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEITGTVTGKDLQEQVLDDM
                     DLERERGITIKSHAIQMNYRQDGVDYILNLIDTPGHVDFSYEVSRSIASCEGALLIVD
                     ATQGVQAQTISNLYMAIDHDLEVIPVM"
     gene            530..1558
                     /locus_tag="PKZ74_09050"
     CDS             530..1558
                     /locus_tag="PKZ74_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPM92945.1"
                     /translation="MSKVRLGIMGFGEIGRHIYRLCMEDDMFEVVAISDYGKPDILAY
                     LLEAEVKGKVKVTLEEGNFINSAAGRARVVTGGKPGDVPWDIFDVDVVIDATGIFKSK
                     KDLEKHLKAGAPHVVLSTLPEEEIDRIVVMGVNENSIDLNDKIVSAGSATTNATALML
                     KFFDEKFGVDYAMLTTVHSYTSDQPLRDKAGVDYRRSRSAAENIIPNETPTPRWIPTI
                     LPKFAGRIEGTALNVPIPNGSLLDLTMIFKNNEVTIDQVNETMYEAANKLPGLIQVAE
                     DPIVSSDVINNRHAVIFDKLATMKSQGKMIKTLVWYHTALAQACRILDVIRTYHNLQE
                     KTGKGGSK"
     gene            1555..2561
                     /gene="gap"
                     /locus_tag="PKZ74_09055"
                     /pseudo
     CDS             1555..2561
                     /gene="gap"
                     /locus_tag="PKZ74_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(2668..5280)
                     /locus_tag="PKZ74_09060"
     CDS             complement(2668..5280)
                     /locus_tag="PKZ74_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM92946.1"
                     /translation="MKRILLTVVLIVAATIHLLATHQRAAEITFRHVTGLTYEVRVIT
                     YTYTSSPADRPTLDIGWGDGTYSTLLRTQKTNLPDNISLNVYEYDPGNGAATNRHTYS
                     SPGTYVIFMEDPNRNLGIVNIPNSVNVPMYVETKLIINPFLGTNNSPVLLNPPIDVGC
                     VNQVFVHNPGAYDPDGDSLSYRMVNCKTAGGIDIPGFFLPQASNSFTIDAVSGDVVWD
                     TPVMQGEYNIAFVVEEWRKGALIGSVTRDMQIEILACNNTPPVIYIPADTCVTAGDTI
                     AFDATAIDPDGDNVWLSATGGPFEVPDSPAELDPDPAAGNDTVSTRFNWISNCSHVRN
                     QPFQVFFKASDDAFPVNLVSFKTLNIKVNCPAPENLTAMAAGSAIHLTWDRIVCSQAS
                     GYRIFRHIGYTGYVPKGCETGVPDSLGYVLIHENQGIADTSFIDDDNGAGLTHGNQYC
                     YMVTAIFADGAESYPSLEACATLKKDLPVITHVSNDSTDLEAGRAFIAWSKPTELDTV
                     QIPGPYQYVLLRSEGPGMVTPQTVATLAGLDDTAYIDNSINLNTSGIPYSYKVNLESL
                     NFGYIGSSRNASSLFLQISPTDEELRLSFIPDVPWENDYYIIFRREEGNTNFDSLGLS
                     TVPFYNDTGLINGTIYCYYIKSVGGYSAAGFIDPIINFSQIACASPYDNVPPCPPELS
                     VTVNCDMAINTLNWINPFDSCSADIFKYLIFYSPYQESDFALIDSTSPASVMIYEHFN
                     NGIITGCYAVMAVDSVGNHSDYSRPVCIDNDTCSTYRLPNVFTPNFDGYNDVFHPFPY
                     TSVERVDMTIFNRWGGMVYETEDPDIGWDGKDMNTNANCSDGVYFYVCRVYEITLLGI
                     VPRELRGSVTIIRD"
     gene            5356..7614
                     /locus_tag="PKZ74_09065"
     CDS             5356..7614
                     /locus_tag="PKZ74_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPM92947.1"
                     /translation="MVTKRHFFMVNADPEIEKKEIVKRYRALLRVWNTKDQDDKKLVR
                     RAFKLAADAHKDMRRHSGEPYIYHPMEVATIVAGEIGLGTTSIVSALLHDVVEDTNYT
                     LEDIRFHFGEHVAQIIDGLTKISEIMDQPATSPQAENFRKILLTLARDVRVILIKLAD
                     RLHNMRTLEALPVDKQLKIASETIYLFAPLAHRLGLFSIKSELEDLALRATEPEIYHT
                     LQKKLQETRTDRIRFTNKFIYPIKKGLRERGVKFQIFTREKSIYSIWQKMKNKEIPFE
                     EVYDLFAVRIVLDVPIPMEKSECWQVYSIITDHYRPNHSRLRDWISIPKANGYEALHT
                     TVMSHAGKWVEVQIRTRRMDEIAERGYAAHWKYKDTIDSETGVEEWLKKVKDMLREEE
                     PDAVDFVNDFKLNLFADEIYVYTPRGEVRTLPSGSSVLDFAYVVHTEIGNHCIGAKVN
                     QVLVSRKHILKNGDQVEVLSSNSTLPEADWLQYAMTAKARANIKQWVREQQRKMTMLG
                     KEKLGVLFQQYNLPDDKASVQRFMSFHNIIKEDDLYYMIASGDLTADDFKNFRVEGEK
                     KNFFSYLSRPFTRQKPGKNRTLTETIVEKLKEKPESLLLSDDITQIKYVLSRCCSPIP
                     GDDVVGFLNENGSIQIHRTNCPDTIDLLSKYGKRIVKAKWKNKESIGFLTGIKIQGVD
                     KKGFIRKVTEVITEKHNINIRSFHLNTSEGMTEGTIMLYLHDTQTLQKLIDNLMKIKE
                     IIKVTRIDRFNE"
     gene            7776..>7926
                     /locus_tag="PKZ74_09070"
     CDS             7776..>7926
                     /locus_tag="PKZ74_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPM92948.1"
                     /translation="MKEREQSDSFETVKKIFTEYLEQRGHRKTPERFTILKEIYNTDG
                     HFDIET"
BASE COUNT         2120 a         1858 c         2042 g         1906 t
ORIGIN      
        1 cattaccggt atcacctcca ggtcatgatc gatggccata taaaggttcg aaatcgtttg
       61 agcctgcacc ccctgcgtcg catcaacgat aagcagcgct ccttcgcagg atgctattga
      121 tcttgaaact tcatacgaga aatccacatg ccccggagta tcgatcaggt tcaggatgta
      181 atcgacgcca tcctgccggt aattcatctg gatagcatga ctcttgattg taattccgcg
      241 ctcacgctcc agatccatat cgtcgagaac ctgttcctgc aaatctttcc cggtcacggt
      301 gccggtgatt tcaagcagac ggtcggccag ggtgcttttt ccgtgatcga tatgggcaat
      361 gatgcagaag ttgcggatgt ttttcatagt gtgcaaaagt agtttttttc ctgattggtt
      421 taaaacttta ttatggttga tatgtttata actgtgattg tgattacctt tgcgcaactt
      481 ttttaaaaag ggcatctaat tgcagaaata ttaattaaaa ttattgatta tgagtaaggt
      541 aagattaggt attatgggat ttggagagat cgggcggcat atctaccgtt tgtgtatgga
      601 ggatgatatg ttcgaagtcg ttgcgatcag cgactatggc aaacccgaca tcctggccta
      661 tttactggaa gccgaagtga aaggaaaggt gaaggttact ctggaagagg gaaacttcat
      721 caactccgca gccggccgtg cccgtgtggt caccggcggc aaaccgggcg atgtgccatg
      781 ggacatcttc gatgtcgatg tggtcatcga tgccacgggt atctttaaaa gcaagaaaga
      841 cctcgagaaa cacctcaaag ccggtgcgcc gcacgtagtg ctgtcaacac ttcccgaaga
      901 ggagattgac cgtatcgtcg ttatgggggt gaacgaaaat tccatagatc tcaacgataa
      961 gattgtatcg gccggttcgg ccactaccaa cgctaccgct ttgatgctga agttcttcga
     1021 cgagaagttc ggcgtcgatt atgccatgct gaccacggtc cattcgtata cttccgacca
     1081 gcctctccgc gacaaagccg gtgtcgatta ccgacgctct cgctctgccg ctgaaaacat
     1141 tatccctaac gaaacgccta ccccgcgctg gatccctacc atcctcccga aattcgcagg
     1201 cagaatcgaa gggaccgcat tgaatgtgcc aattccgaac ggttctctcc tcgatctgac
     1261 gatgatcttt aaaaataatg aagtgacgat cgaccaggtg aacgaaacga tgtacgaagc
     1321 ggcaaacaag cttcccggtc ttatccaggt agccgaagac ccgatcgttt ccagcgacgt
     1381 gatcaacaac cgccacgcgg tgatcttcga caagctggcc acgatgaaat cgcagggcaa
     1441 aatgatcaag accctggtgt ggtaccatac cgcactggca caggcctgcc gcatcctcga
     1501 tgtgatcagg acctatcata atttacagga aaaaacaggg aaaggaggtt ccaaatgaga
     1561 gtagcaatta atggattcgg aagaatcggt cgttcagtat atagaatcct ggatgaagat
     1621 ccaaacatcg atgtcgttgc catcaacgac ctttatgaca atgacgtgct ggcatacctg
     1681 ctgaagtacg ataccgtcat gtcgaaattc tatgcagaag cccgggtgga aggcgactat
     1741 cttgtcactc ccagggaaaa ggtgctgatg ctgtctcaaa gcgatatggc gcagcttccc
     1801 tggaaagagc ttgatatcga tgtggtagtg gaagccacca gcgtattccg gaaaaaatca
     1861 cagctcgaag cccatctgag tgccggcgca aggcgtgtat cctgaccgtt ccatcgaagg
     1921 acgaaatcga tttcacggtt gttatcggag tgaatgaaga cggccttcag cctgaacacc
     1981 gtattgtgtc gaacgccagt tgcacaacca actgcctggc ccccgtagcc aaagtgctca
     2041 acgattcatt cggcattgtc gaaggattga tgaccaccac acatgcatat accaacgatc
     2101 agcgcctggc cgacgtccct cataaggact ggcgccgtgg acgcgctgcc gccgagaaca
     2161 tcgttcttac aactaccggc gccgccaaag cagtcggcaa agtgattccc gaactgaaag
     2221 gaaagcttga tgggcttgct tcacgcgtac ccgttccgga tggctctgtc gtcgacctga
     2281 tcgtagaagt gggaaaagat gtcaccgtgg aagatgtgaa caatgccatg aaggcggccg
     2341 ccgctaccga aaggatgaag aacgtgctgc tttattccga cttcaaatgg gtatcttccg
     2401 atatcgtcgg taatccctat tcctcgattt tcgatccgga atacacccgc gtgatcggaa
     2461 accgcctggt gaaagtcctg agctggtacg acaacgaatg gggctacagt aaccgcgtcg
     2521 tcgacctgat caggcttttc aaggcgatgg ataacaagta aagttctttg gatatttcat
     2581 ccgtgtagat tcgtaaaatc agtgtaatcc gtgttctgtt ttataaagaa cacgaataac
     2641 acgaattatg cggatttaca cggattttta atctcttatt atcgtaaccg atcctctcaa
     2701 ttccctcgga acaatgccca gcagggtgat ctcataaacc cggcagacgt agaaataaac
     2761 accgtcgctg caattggcgt tggtgttcat gtctttacca tcccagccga tatcggggtc
     2821 ttcggtttca taaaccatcc cgccccagcg gttgaaaatc gtcatatcta ctctttcaac
     2881 cgatgtgtaa ggaaatggat ggaaaacatc gttataacca tcgaaattag gtgtaaaaac
     2941 attcggcagc cggtaagttg aacaggtgtc gttatcaatg cagacaggcc gactgtaatc
     3001 gctgtggtta ccgactgaat cgaccgccat gactgcatag cagccagtta taataccgtt
     3061 gttgaagtgt tcgtaaatca tcactgatgc cgggctggtg gaatcgatca atgcgaaatc
     3121 actttcctgg taaggtgaat aaaagatcag gtatttaaag atatctgccg aacatgaatc
     3181 gaacgggttg atccagttta acgtattgat tgccatatcg cagttcacgg tcaccgacaa
     3241 ctccgggggg cagggaggga cgttgtcgta cggtgatgca caggctatct gtgaaaaatt
     3301 gatgatagga tcgataaaac ctgcagcgga gtagcctccg accgatttaa tataatagca
     3361 gtaaatcgta ccgttgatca gcccggtatc attatagaat ggtacggtac tcaaacccag
     3421 cgagtcgaaa ttcgtattgc cttcttctct tctgaaaatg atgtaataat cgttttccca
     3481 tgggacatcc gggataaaag ataaccgaag ctcttcatct gtgggtgaaa tttgcagaaa
     3541 aagagaagat gcattccggc tactgccgat atacccgaaa ttgaggcttt cgaggttaac
     3601 cttgtagctg tatgggattc cgctggtatt caggttgatt gaattatcta tataagcagt
     3661 gtcatcaagt ccggccaggg tggcgacagt ctgcggggtt accattccgg gtccctctga
     3721 gcgaaggagc acatattggt aaggccccgg gatctgaacg gtgtcgagtt cggtaggctt
     3781 tgaccaggca ataaaagcgc gtcctgcttc caggtcagtg ctatcgttgc tgacatgggt
     3841 gatcacgggt aaatcctttt tcaatgtggc gcatgcttcc agggaagggt agctttcggc
     3901 gccatcggca aaaatggctg tgaccatgta gcaatactgg tttccatggg taaggccggc
     3961 cccgttgtca tcgtcaataa agcttgtatc ggcaattccc tggttctcgt gaataagcac
     4021 ataacccagg ctgtcgggca ctcctgtttc acaacccttt ggaacatacc cggtatagcc
     4081 gatgtgcctg aatatcctgt atccgcttgc ctggctgcaa acgatccggt cccaggtgag
     4141 atggatcgca ctcccggccg ccattgccgt aagattctcc ggtgcagggc aattgacttt
     4201 gatattaagg gttttaaaac tgaccaggtt gacgggaaaa gcatcatcgg acgctttgaa
     4261 aaagacctgg aacggttggt tccggacatg gctgcagttg ctgatccagt tgaaccgtgt
     4321 cgataccgta tcgtttcctg ctgcaggatc gggatccagt tcagcgggcg aatcgggtac
     4381 ttcgaagggg ccgcccgttg ccgacagcca tacgttgtcg ccgtcagggt ctatggctgt
     4441 ggcatcaaac gcaatggtgt cgccggctgt cacgcaggtg tcagcaggga tgtagatcac
     4501 cggcggggta ttgttgcagg ccaggatctc gatttgcata tcgcgggtaa cgcttccgat
     4561 cagcgcccct tttctccatt cttcaacgac aaaggcaatg ttgtattccc cttgcatgac
     4621 cggtgtgtcc caaaccacat caccgctgac ggcatcgatg gtaaaggagt tgctggcctg
     4681 gggcaggaaa aatcccggaa tatctattcc gccggccgtt ttgcaattga ccatcctgta
     4741 ggaaaggctg tcgccatccg ggtcataggc cccgggatta tgaacgaata cctggttcac
     4801 acatcccaca tcgatcgggg gattcagcaa taccggagag ttatttgtac ccagaaaggg
     4861 attgatgatg agtttggtct ccacgtacat cggaacattt accgaattgg gaatatttac
     4921 gattcccagg ttccggttag gatcttccat gaaaatcaca taggtgcccg gagagctgta
     4981 agtatgccgg ttggttgcag cgccgtttcc cggatcgtat tcgtaaacat tgagtgagat
     5041 attatccggt aaattggttt tctgtgtgcg cagcagggtg ctgtaggtcc cgtcgcccca
     5101 gccgatatcg agggtaggac ggtcggcagg gcttgaggta taggtatagg taatgaccct
     5161 cacttcgtaa gtcaggcccg ttacatgccg gaaggtgatc tctgccgcgc gctgatgggt
     5221 tgccagcaaa tggattgttg cggctacgat caaaaccacg gtgagtaaaa ttcttttcat
     5281 tattctataa cgtaacaaaa gtccaaattt agataaattc catccttcgg agcaaaattt
     5341 cttattcgta actttgtggt gaccaagcgt catttcttta tggttaatgc ggatcctgaa
     5401 atagagaaga aagaaatagt gaaacggtac cgggctttgc tcagggtctg gaacacaaag
     5461 gatcaggatg acaaaaagct ggttcgccgg gcgtttaagc ttgcggcgga tgcccataaa
     5521 gacatgaggc gccattccgg cgagccctac atttatcatc ccatggaggt agccacgatt
     5581 gtggccggtg aaatcggcct gggcacgacc tctatcgtca gcgcacttct tcacgacgtg
     5641 gtcgaagata ccaattatac cctggaagat atccgttttc acttcgggga gcatgttgcg
     5701 caaatcatcg atggcctgac taaaattagc gagataatgg atcaaccggc gacgtcgcca
     5761 caggcggaga atttccgcaa gatcctgctg acgctggccc gggatgtcag ggtaattttg
     5821 atcaaactag ccgaccggtt gcataacatg agaacgctgg aggcccttcc ggttgacaaa
     5881 cagttgaaaa tcgcttccga aacaatatat ctgttcgcgc cgctggccca ccggttagga
     5941 ctgttttcga taaaaagcga attggaagac ctggctttga gggctaccga acctgagatt
     6001 tatcatacgc ttcagaaaaa actccaggaa acgcggacag accgcatccg ttttaccaat
     6061 aaattcatct acccgataaa aaaagggctc agggaaaggg gcgtcaagtt ccagatcttt
     6121 acccgggaga agagcattta ctccatctgg cagaaaatga agaacaaaga gatccctttc
     6181 gaggaggtgt acgatttgtt tgcagtcagg attgtactgg atgttcccat tcccatggaa
     6241 aaatcggagt gctggcaggt ctattcgatc attaccgatc attaccggcc caatcacagc
     6301 cgcctgcgcg actggatctc cattcccaaa gccaacggtt atgaggccct tcatacaacc
     6361 gtcatgagcc atgccggtaa atgggtggag gtgcagattc ggacccggag gatggacgag
     6421 atcgcggaaa gaggatatgc tgcccactgg aaatacaagg ataccatcga cagcgagaca
     6481 ggcgtggagg aatggctgaa aaaggtaaag gacatgctgc gagaagaaga gccggatgcg
     6541 gtagacttcg tcaatgattt caaactgaac ctttttgcgg atgaaatcta tgtttatacc
     6601 cccaggggtg aagtaaggac attgccttcg ggttcttccg tgctcgattt tgcctacgta
     6661 gtccacaccg agatcgggaa tcattgcatc ggggcaaaag tcaaccaggt actggtttcc
     6721 cgtaagcata tcctgaagaa cggcgaccag gtggaggtct tatcttccaa cagtaccctg
     6781 ccggaggccg actggctcca atacgccatg acggctaaag cgcgggccaa tataaagcaa
     6841 tgggtcaggg aacagcagag gaaaatgacc atgctcggga aagaaaaact gggcgtgtta
     6901 ttccaacaat ataacctgcc cgatgataaa gcttcggtcc agcggttcat gagcttccac
     6961 aacatcatta aggaagatga cctgtattat atgattgcca gcggcgacct taccgccgat
     7021 gattttaaaa atttcagggt ggaaggggag aagaagaact tcttcagcta tctcagccgg
     7081 ccgttcaccc ggcagaaacc ggggaaaaac aggaccctga ccgaaacaat tgtcgagaaa
     7141 ctgaaggaga agcccgaatc attgcttctg agtgatgata tcacccagat caaatatgtt
     7201 ttgtcgcgct gctgcagtcc tatccccggt gatgatgtgg tcggtttcct gaacgagaac
     7261 ggctcgatcc agatccaccg gaccaactgc ccggatacga tcgacctgct ttcgaaatat
     7321 ggcaagcgga tcgtgaaagc gaaatggaaa aataaggagt cgattggctt tttgacaggg
     7381 ataaagatac aaggagtgga taaaaaggga ttcatccgca aggtgaccga agtgatcacc
     7441 gaaaagcaca acatcaatat ccgttctttc cacctgaata cttccgaagg gatgacggaa
     7501 gggacgatca tgctctatct tcatgatact cagacactac agaagctcat tgataacctg
     7561 atgaagataa aggagatcat caaagtgacg aggatcgaca ggttcaatga gtaggagggc
     7621 agtaggcagt cggcagtagg cagtcggcag ttccgttgcc agggtgaggg ttggcgtcat
     7681 tacgacggag ggggaaacat gttaaaaaat tatttttatt tataccaaat agtaataata
     7741 atttttctac ttttatgcac ttttctgtaa tgtgtatgaa agaaagagaa caaagcgatt
     7801 cattcgagac ggttaagaag attttcacgg aatacctgga gcagcgaggc cacaggaaaa
     7861 cgccggaacg gttcaccatc ctgaaggaaa tttataatac cgacgggcac ttcgatatcg
     7921 aaaccc
//
LOCUS       DAOPNH010000157         7915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10249_length_7915_cov_4.631425, whole genome shotgun sequence.
ACCESSION   DAOPNH010000157 DAOPNH010000000
VERSION     DAOPNH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_7915_cov_4.631425"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKZ74_09075"
     CDS             <1..679
                     /locus_tag="PKZ74_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPM92949.1"
                     /translation="IGARVDKQSVPNSIPLVAIGSGLLWFGWYGFNAGSEVAVDHVTA
                     IAFLNTDLAASFATIAWVVVEWIRERRPKFVGLLTGSIAGLATITPCAGFVPVWAAPI
                     IGTAAGLMCYVAVQWKNKMKWDDALDVWGVHGVGGFLGTILLGVFASKAVNSAGTDGL
                     IFGGGTFFWKQVLAVVAASAYAFIFTYIMLALINKISRVRVSEVEEKAGLDIALHGEK
                     AYDEGAL"
     gene            complement(779..3565)
                     /locus_tag="PKZ74_09080"
     CDS             complement(779..3565)
                     /locus_tag="PKZ74_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM92950.1"
                     /translation="MKTRPTRLIVAAITAFAFLSSLFLLNRNQTERERYEQFLLDQAI
                     GIDIAPAGESEKEEGKSPDEPQMAAFQDFLQTVDPVEKRVPVERLHQAYFNLNNQNKE
                     DRLKTMDPLQWDIIPSDMGGRTRCIMYDPNSASGNKVWAGAVTGGLWYNNDITGSQDW
                     VPVDDFWPSLSISGITCDPNNPQVFYAGTGEAFTARVIYRESSGIGAGIFKSENAGNT
                     WTQLPATAGWKYVTDVEVRDENGLSVIYAGVASGEYHGTQQSEPTDGLFRSVDGGTTW
                     IQVLPAVEGSENPYAVADIEITAGNRILIGTLPNLNQEGGATILRSDLGTAGSWTVYD
                     DYVAIIQSYPDFPIPNRVMLASAPSDPDVAYALLDAGYINSSNGFIYTQGLFILRTGN
                     GGATWVSKAIPTGGDYYWATIGWHALTAGVDPNNADALYIGGLDVYKSDDGANSWIQV
                     SNWWYMYQGGGDRYVHADIHDIDYKPGSSDELVITTDGGVFYTDEAQSDGPAFQEKNN
                     GYGSLQFYTCDIHSNPLQNKAVGGLQDNGTLYFTGSPLTIFNMIDGGDGACCFIDQNQ
                     PQYMITSVYYNQYSLWLNGNYYASMADWSSGTFISPADYDDASNILYANACSFGGSQA
                     NRILRIKGIPDNISGSYVNLNTGLNVYFSSVICSPYATAGTSTLFAGSLSGRLFKATN
                     AQASPTVTELTGAGFPLGAISCVAIGGSEDTLLVTFSNYGVSSVWQTCDGGATWAEKE
                     ANLPDMPVRWAIYHPLSSNYAMLATELGIWTTTNLQDDAPVWEQDIQGLANVRVDMLQ
                     MRMSDYQVLAATHGRGLARATWDIGVGVDEETGGPGDGENDLVEVWPNPTQGKFQITS
                     TKIQTNSKIQTIIKQVEIVDLDGNIVISNNNRTIEQWNSEPIELNISNYPSGIYLVRI
                     ISGNKLIVKKIVKS"
     gene            3945..7721
                     /locus_tag="PKZ74_09085"
     CDS             3945..7721
                     /locus_tag="PKZ74_09085"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92951.1"
                     /translation="MKTLVTFLKISFLCVINIIIWQQSIFSTVRPDEITTVTLPSIET
                     GIIPEDIIYIEEFNRFYIFGNNKLIIIEYSTTESKYVISGSICLFGTLLQDCDRYGNN
                     IYPKKNITNETPKNKYLAYDPDNKNLYVLRPDFRIVQIDIINNVNNGVKISALPAFQP
                     FAFETSKIYIRNGILYWQQYGQWGDPPTKKQFFGSYNINDEFSQIINNYTDYQASILD
                     FVVDDNFIYLLYNPGTGSQIQRRYISSFAVYGTTITANFQLGEFYYGSNNRIYVYGFG
                     PGAGKVYVIDPTDLTSTLSPEIIDHYNNMEIPPISCATYDNINHFLYLGTCYSAGQDD
                     IFIYNYDINVDEHNYINKLATNNIPSKSENKPYYLFCANEKAYASKESEIVRIDYKGD
                     YKFESSQVIEKVNNYFYTCALDEQLDNVLALNLEGVIHVIDNTGQYFDNPDIQVGIKV
                     NDAYHIPFKDIVCFYTRDLFQDNHLVVYDNHSKVIIADIKNEHPISDVIYNPFTSEIL
                     VSYYSGEVNYLSRYSYTPPATINEEKVYFQNSNDNYCGKIFIDPNHHLYCAVGLDNIN
                     DAKILFINADNYSQINSFALNGVTNLTDFKVSFTYHKPNSFVYSTIMQFSDDFNYSDI
                     VEINPSGIGSLIHLISQHDLPVKIESCPVHNKLYVQYMNDNHITRIKWIDNTIDYFDD
                     FNLPNVCILDIAYDDHLDFLYFTNGFSQTGFIYCDLDIFNEERVFDNGHSYYPIIFNK
                     KNNFLYSYCPNDHPARINKLMSSEPLFWSESNVITLNNTINRKSEIGYMNTLSVDDYK
                     NFLFIPNGSQCNFTVVRCTPERRYLGQGIPNPYEPFNYNYISFPSLERLEGVEDNPVS
                     AIDVLSKFEPFTESPVITMESRVPGNSEPYTIYHPHDGEWSGTLTDVFSSRGYALQHN
                     VPSGQVCYIPMEGMRLDPGYDQIKLYYGANIENWTGYFIDETQNIFNALGEVEQDLNL
                     IIAQCWACTRQFWSGDPGINQSWMCRTAIGAENIPVGGISYGTMVRLTLKPNVPFRTF
                     GWSSGQEPYPIITKSDPVHFIFNSNSLYTPIFIELDTSARPLEIGAFIGNTCIGATTV
                     NLDDTIIMVPAYNNGDTTSIIEFEEYFGDLKSPNRNRIINYRSFNSKINKITDNTIRI
                     CSPENGAYFVSLKKEDIIEEQNIQTSFMCYPNPYQAGSLTLTANLDSASDIQIEIFDA
                     RSRLCAFYEIGDQPSGLFNCNLENLEISAQLSPGIYLVKMKTGVRELYTKLIIR"
BASE COUNT         2185 a         1601 c         1770 g         2359 t
ORIGIN      
        1 catcggcgcc agggtcgata agcaatctgt tccgaacagt atacccctgg tggctatcgg
       61 cagcggtttg ctctggttcg gatggtacgg cttcaatgcg ggtagtgagg tggcagtcga
      121 tcatgttaca gcaatagcgt tcttaaatac cgatctggcc gcatcttttg caaccatagc
      181 atgggtcgtg gttgaatgga tccgggagcg tcgtccgaag tttgtcgggt tgcttaccgg
      241 ttccatcgcc ggcctggcaa caatcactcc gtgcgccggt tttgtgccgg tttgggccgc
      301 tccgatcatt gggaccgcag ccggactgat gtgttatgtt gccgttcaat ggaagaataa
      361 gatgaaatgg gatgatgcac tggatgtctg gggcgttcac ggtgtaggtg ggttcctggg
      421 aacgatactg ctcggcgttt ttgcttccaa agcggtgaac agcgccggaa ccgacgggtt
      481 gatctttggc ggcggcactt tcttctggaa acaggtcctg gctgttgttg cagcatctgc
      541 atacgccttt atcttcacct atatcatgct ggcattgatc aataaaatct ctcgtgtgag
      601 ggtctcggaa gttgaagaga aagccggatt ggatatagcc ctccacggag aaaaagctta
      661 cgacgaagga gcgttgtagc ttcttttttt caccacaata ggcacatagg acacatagat
      721 tacacaatag aattctatgt gattcttatg tgttctattg tgcctatgtg gttaataatc
      781 atgacttaac gatcttctta actatcaatt tattgcctga gatgatcctg accaggtaaa
      841 tcccggaagg gtagttgctg atgtttagtt ctataggttc actgttccat tgttcaattg
      901 ttcgattgtt attggagatg acgatattcc catctaaatc gactatttca acctgtttta
      961 ttatggtttg aattttcgaa tttgtttgga ttttggtgct tgtaatttgg aatttcccct
     1021 gtgtggggtt cggccacacc tccaccaaat cattctcccc gtcgccgggt ccccctgtct
     1081 cctcatccac tcccacgccg atatcccagg tagcccttgc caggcctcgg ccatgggtcg
     1141 ctgccaggac ctggtaatca gacatccgca tctgcagcat atccaccctc acgttggcca
     1201 gcccttggat atcctgttcc cagaccggcg catcatcctg tagattggta gtggtccaga
     1261 tgcccagttc tgtggccagc atggcgtagt tgctgctcag cgggtggtat atcgcccagc
     1321 ggacgggcat gtcgggcagg ttggcctctt tttcggccca ggtggcgccg ccgtcgcagg
     1381 tctgccagac agacgacaca ccgtagttgg aaaaagtcac cagcagcgta tcctcagaac
     1441 cgccgatggc tacgcaggag atggctccca gggggaagcc ggcgccggtg agttcggtga
     1501 cagtcgggga ggcctgggcg ttcgtagctt tgaataacct gccggagaga gagccggcga
     1561 aaagggtaga ggtacctgca gtggcatacg gactgcagat caccgatgag aaatagacat
     1621 tcagcccggt gttcaggttg acatacgatc cggagatatt atcggggatg cctttgatcc
     1681 tcaggatgcg gttggcctgg cttccgccga aagaacaggc attggcatag aggatgttgc
     1741 tggcatcatc gtaatcggcc gggctgataa aagtgccgct cgaccagtcg gccattgagg
     1801 cgtaataatt gccgttcagc cagagcgagt actggttgta atagaccgat gtgatcatgt
     1861 attgcggctg gttctggtcg ataaagcagc atgccccgtc gcccccatcg atcatgttaa
     1921 agatggtcag cggggagccg gtgaagtaca gcgtcccgtt gtcctgcagg ccgccgacag
     1981 ctttgttctg caacggattg gaatggatgt cgcaggtgta gaactgcaat gagccgtagc
     2041 cgttgttctt ttcctggaag gcaggtccgt cggattgggc ctcgtcggtg taaaagacgc
     2101 ccccgtcggt agtgatcacc agctcgtcgg atgagccggg tttgtagtcg atatcgtgaa
     2161 tatcggcgtg cacgtagcgg tcgccgcccc cctggtacat gtaccaccag ttcgacacct
     2221 ggatccagct gtttgccccg tcgtcggatt tatagacgtc gaggccgcca atgtagaggg
     2281 catctgcgtt gttgggatca acgccggccg tcagggcatg ccagccgatg gtggcccagt
     2341 agtaatcgcc gccggtaggt atcgctttcg agacccatgt ggcaccgccg ttaccggtgc
     2401 ggaggataaa caaaccctgg gtgtagataa aaccattgct ggaattgatg tacccggcgt
     2461 cgagcagcgc ataggccaca tccggatccg acggggcgct ggccagcatc acccggttgg
     2521 ggatcgggaa atcgggatat gactggatga tcgccacata gtcgtcgtaa actgtccatg
     2581 atccggctgt ccccaggtcg gaacggagga tcgttgcgcc gccctcctgg ttgaggttag
     2641 gcagcgtgcc gatcaggata cggttgccgg cggtaatttc gatgtcggca acggcgtagg
     2701 ggttctccga tccttcgact gcgggtagca cctggatcca ggtcgtgccg ccgtcgaccg
     2761 accggaacag cccgtcggta ggctcgcttt gctgggtgcc gtggtattcg cccgaagcaa
     2821 cgccggcata gatcacgctg agtccgtttt cgtcgcgtac ctccacgtcg gtgacatact
     2881 tccagccagc ggtagcgggg agttgcgtcc aggtgttgcc ggcattttcc gatttgaaga
     2941 tccctgcgcc gatcccggaa gattcgcggt agatcacccg agcggtaaat gcttcgcctg
     3001 taccggcgta aaatacctgc gggttgttcg gatcgcaggt gatgccgctg atggagagcg
     3061 acggccagaa atcgtcgacc ggcacccagt cctggctgcc ggtaatatcg ttgttatacc
     3121 acaggccgcc ggttacggcc ccggcccaga ccttattgcc cgaagcgctg ttagggtcgt
     3181 acatgatgca ccgtgtcctg ccgcccatat ccgacgggat gatgtcccac tgcaagggat
     3241 ccatggtctt gagcctgtcc tccttattct ggttatttaa gttaaaataa gcctggtgga
     3301 gacgttcgac gggaaccctt ttttcaacag ggtccaccgt ctgtagaaaa tcctggaaag
     3361 ccgccatctg cggttcatcc ggcgattttc cctcttcttt ctctgattcg cctgccggtg
     3421 cgatatcgat cccgatagcc tggtccagga ggaactgctc gtatctttcc cgttcggttt
     3481 gattccggtt gaggaggaat aaactgctta ggaaggcaaa agcagtgatg gcagcgacga
     3541 tcaggcgggt tggtctggtt ttcatcttaa aggcgattta ttattcttga aatgggaggg
     3601 taaaggtatg gataaagtta aatcaacgaa atatataatt ccaaggtggc gggtggctta
     3661 tcattgagtt ggcagcctgc tatttctttt actggagact tggtacatat cgtacgcatg
     3721 ttattatttg gaatgattcc atatttcttg tttctattga cttttaaatt ttttatctta
     3781 tctttgatac tatgaaataa acattggttg atgtcaggca atcaatgttt attctatccg
     3841 ataaattcca gccgcaattt tattggaact gaattttgtt cagttaaatc ttgattggtg
     3901 tcatatatta aagatataat tattaaccat taaaaattgc gcatatgaaa accttggtca
     3961 cttttcttaa aatctcattt ttgtgtgtca taaatatcat tatatggcaa caatccattt
     4021 tctctacagt caggcctgat gaaatcacaa cagtgactct ccctagtata gaaacaggta
     4081 taattccgga agatataatt tatattgagg aatttaacag gttttatatc tttggtaaca
     4141 ataaacttat aatcatagaa tatagcacta ctgaatcaaa atatgttatt tctggaagta
     4201 tatgcctttt tggaacttta cttcaagatt gtgatagata tggtaataat atttacccca
     4261 aaaaaaatat aacaaatgaa accccaaaaa ataagtatct tgcatatgat cctgataata
     4321 agaacttata tgttttaagg ccggatttca gaatagttca gattgatata ataaataatg
     4381 tcaataatgg agttaaaatt tccgcattgc ctgctttcca accttttgca ttcgagactt
     4441 cgaagatata tataagaaat ggaatcttat attggcaaca atatggacaa tggggagatc
     4501 ccccgacaaa aaagcaattt tttggtagtt ataatattaa tgatgaattt tcgcaaataa
     4561 taaacaatta tactgattat caagctagta ttctggattt tgttgttgat gacaatttta
     4621 tatacttatt atataatcca ggaacaggtt cacagattca aagaaggtat atatcaagtt
     4681 ttgccgttta tggaactacg attacagcaa attttcaact tggagaattc tattatggtt
     4741 cgaataatag aatatatgtt tatggatttg gaccaggtgc aggaaaagtt tatgtaattg
     4801 atccgactga tcttacttca acattatctc cggaaataat agaccactat aacaacatgg
     4861 agattcctcc aatctcttgt gcaacttatg ataatattaa tcattttctt tatctaggca
     4921 cttgttattc cgcgggacaa gatgatattt ttatatacaa ctatgatatt aacgtggatg
     4981 agcataatta tattaataaa ttggcgacaa acaatattcc aagtaaaagt gaaaacaaac
     5041 catattactt attttgtgct aatgaaaaag catatgcttc taaagaaagt gaaatcgtaa
     5101 ggatcgacta taaaggagat tataaatttg aatcatctca agtaatagaa aaggtaaata
     5161 attactttta tacttgcgct ttggatgagc aattggacaa cgtgttggct ttgaacctgg
     5221 aaggagttat ccatgtaatt gataatacag gtcaatattt cgacaacccg gatattcaag
     5281 tcggtataaa agtaaatgat gcgtatcata tcccatttaa agatattgtc tgcttttata
     5341 cacgggattt atttcaagac aaccatttgg tggtttacga taatcattca aaggtaatta
     5401 ttgctgatat aaaaaatgaa catccaattt ccgatgtgat atataatcct ttcacttcag
     5461 aaatattggt atcatattat tcaggagagg ttaactattt aagtagatat agttatactc
     5521 caccggcgac aataaatgaa gaaaaagtat atttccagaa tagcaatgat aattattgtg
     5581 gtaaaatatt tattgatcct aatcatcact tatattgtgc tgtaggcctg gacaatataa
     5641 atgacgctaa gatattattt ataaatgcgg acaattattc tcaaatcaat agttttgcat
     5701 taaatggtgt tacaaatctc accgatttta aagtttcttt tacgtaccat aaaccaaatt
     5761 cttttgtgta ttctaccata atgcaatttt ctgacgattt taattattct gatattgttg
     5821 agattaatcc ttctggaatt ggctccttga ttcatttaat ttcgcaacat gatcttccgg
     5881 taaaaattga aagttgccct gttcataata aactatatgt ccaatacatg aatgataacc
     5941 atattacgag aataaaatgg atagataaca ctattgatta tttcgatgac tttaaccttc
     6001 ctaatgtctg tattttagat atagcatatg atgatcacct tgattttctt tatttcacaa
     6061 atggtttttc acaaactggt tttatttact gtgacttgga tatatttaat gaggagcggg
     6121 ttttcgataa tggccattca tattacccta tcatatttaa taagaagaac aatttcttat
     6181 attcttattg ccctaatgat cacccagcca gaataaataa gttaatgtca tcagaacctt
     6241 tattctggtc agaatcaaat gtaatcacat tgaataatac aattaataga aaatctgaaa
     6301 tcggatatat gaacaccctt tctgttgatg attataaaaa tttcctattt ataccaaatg
     6361 ggtctcaatg caattttacc gttgtaagat gcactcccga aagaagatat ttaggtcagg
     6421 gcattcctaa tccttatgag cccttcaatt ataattatat tagcttccct tctttagaga
     6481 gactggaggg agttgaagac aatccagtga gtgcaataga tgttctctca aaatttgagc
     6541 catttacaga atcaccagta ataacaatgg aaagccgagt acctggcaat tcagagccat
     6601 atacaatata tcatccacat gatggggaat ggtcggggac tttgactgat gtatttagct
     6661 caagagggta tgctttgcag cataatgttc catccggaca agtatgttat ataccaatgg
     6721 aaggaatgcg gttagatcct ggatatgacc aaataaaact atattatggc gcaaatattg
     6781 agaactggac aggatatttt atagatgaaa cccaaaatat ttttaatgca ttaggagagg
     6841 tcgaacaaga ccttaatctc atcattgctc aatgctgggc atgtacacgc caattttggt
     6901 ctggtgatcc tggaataaat caatcatgga tgtgtcgtac tgcaataggc gcagaaaata
     6961 ttcctgtcgg tggaatttca tacggtacga tggtgagact gacattaaaa ccaaatgtac
     7021 ctttccggac gtttgggtgg agttcaggtc aagagcctta tccaataata acaaaatctg
     7081 atcctgttca ttttatcttt aattcaaact ctttatatac tccaattttt attgaattag
     7141 acacatcagc aaggcccttg gaaatcggtg catttatcgg taatacctgc atcggtgcga
     7201 caactgtgaa tttagacgat actatcataa tggttccagc atacaataat ggcgatacca
     7261 cttcaataat tgaatttgaa gaatactttg gcgatttgaa atcacctaat aggaatagga
     7321 ttataaatta tcgatccttt aattctaaaa taaacaagat tactgataat acaatacgaa
     7381 tttgcagtcc agagaatgga gcctattttg tttctctcaa aaaggaagat ataattgaag
     7441 agcaaaatat tcaaaccagt tttatgtgtt atcctaatcc atatcaagca ggttctttaa
     7501 ctttaactgc taaccttgat tctgcaagtg atatccaaat cgaaatcttt gatgctagaa
     7561 gtagattatg tgctttctat gaaataggag accaaccgtc cgggcttttt aattgtaatc
     7621 ttgaaaatct ggaaatatcg gctcaattat ctcccggaat ttatttagta aagatgaaaa
     7681 caggggtccg tgaattatat acaaagctga tcatccgata ataattacga tttgaattat
     7741 ctttatagaa tatttattat tcatgcttaa atcattattc tctttacttc ttacgttggt
     7801 attaacaaat gccattgccg atacgcttac ggttaaactg gatggtaccg gtgattatac
     7861 cgacattcag aatgccatcg gttggtcttc atccggagac gttatcctga tctat
//
LOCUS       DAOPNH010000158         7878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10315_length_7878_cov_5.563339, whole genome shotgun sequence.
ACCESSION   DAOPNH010000158 DAOPNH010000000
VERSION     DAOPNH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10315_length_7878_cov_5.563339"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKZ74_09090"
     CDS             complement(<1..618)
                     /locus_tag="PKZ74_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92952.1"
                     /translation="MRTLQFILQKEFIQLFRNKVMVRIIFIIPFVQMLILAYTATFEI
                     KTVRLHVADLDRTKTSRDLAGHFSGSPFYKIVAYSHSEKLSQDDILKNRAHQVLTIPE
                     GFEKDLLTSGNAKVQIVTNAIDGAAAALMNAYTLSIIQDFNINLLVESTGIQAEEPIK
                     TTRSFWFNPELNYINYMVPGILVLLVTIIGMFMSGMNLVKEKETGT"
     gene            complement(621..1721)
                     /locus_tag="PKZ74_09095"
     CDS             complement(621..1721)
                     /locus_tag="PKZ74_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM92953.1"
                     /translation="MRGFIIKEFFHIFRDVRTMIILFGMPVIQILLFGFALTNEIRNA
                     RIAVLDHSKDDVTTEIISRITSSGYFILDEYLESEEQIEDLFRKGKIKEVIIFEENFA
                     EKLVKDKTAGIQIIGDASDPNTANLLANYTNAIIKDYQAGMLLNQASIPMVITPEPKM
                     LFNPELKGVFMFVPGIITILLMLVCAMMTSISIAKEKELGTMEILLVSPLKPLQIILG
                     KVTPYILLSLINALTILALGYFVFGMPMRGNIVLLLLETILFIIMALSMGILISTIAK
                     TQQVALMVSLAGLMLPTILLSGFIFPVENMPWALRTLSNIMPSKWFIIIIKNIMLKGI
                     DITYIWKETLILVFMTLFFITLSVKNFKIRLA"
     gene            complement(1734..2468)
                     /locus_tag="PKZ74_09100"
     CDS             complement(1734..2468)
                     /locus_tag="PKZ74_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92954.1"
                     /translation="MTNDHHIIEVENLVKKFGSFVANDHLNFYVKKGEIFGFLGANGA
                     GKTTAIKILCGLWKPTSGRIQVAGFDVYKETEKIKRNIGYMSQKFSLYEDMTIAENFT
                     FYGGIYGMRMPGIRKKMSFFLEKLGIVEYKDRVISDLPLGWKQKLAFSIAIMHDPAIV
                     FLDEPTSGVDPLTRRQFWDMIYEAAHDGITVFLTTHYMDEAEYCERVSIMVDGRIEAL
                     GTPSEQKKQFGAESMEEVFIRLARKQ"
     gene            complement(2461..3411)
                     /locus_tag="PKZ74_09105"
     CDS             complement(2461..3411)
                     /locus_tag="PKZ74_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM92955.1"
                     /translation="MESVRITGLSKSYGKVRALDSISLSVDKGELFGFIGPDGSGKTT
                     LFRILTTLLLPDSGQATVEGFDTVKEYRQIRKIAGYMPGRFSLYQDLSVEENLKFFAS
                     IFKTTIQENYYLIRDIYNQIEPFKDRPAGKLSGGMKQKLALSCALIHKPAVLFLDEPT
                     TGVDAVSRKEFWDMLARLKKEGITILVSTPYMDEAGRCDRVALIQQGKILGIDTPRGI
                     VGAYKDKLLAVRSSGMYKLLFDVQEFPRTIRAYRSGEFIHCSVKDENAGDELRNFLAG
                     KGHEMIEVRNISPDVEDCFLALMTNDRSFGTSPSGQATND"
     gene            complement(3424..>4194)
                     /locus_tag="PKZ74_09110"
     CDS             complement(3424..>4194)
                     /locus_tag="PKZ74_09110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPM92956.1"
                     /translation="NGQIKYLNIEEGMQVNVGDTVAMTDTIDLSLKREQLVSQSKVIL
                     ARLQNIDSQISVQEQQKKNLLVDRERVGNLLKDGAATQKQMDDISGNLDLISKQIRAT
                     AVQKESVRAELGTLEAQVRQVEESMNKCFIVSPLSGTVLVKFAEAGEITTPGKPVCKV
                     ANLETLELKVYISGDQLPYLRLGDNVDVMIDKDKSGFTNLTGRVSWISPKAEFTPKTI
                     QTKKERVNLVYAAKVLVPNDGSLKIGMPGEINFMPVTD"
     assembly_gap    4195..4294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4295..5389)
                     /locus_tag="PKZ74_09115"
     CDS             complement(<4295..5389)
                     /locus_tag="PKZ74_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM92957.1"
                     /translation="MKTALLLLIATFMLTSASAQSDTLSLADCLSKAEASHPLFQQYD
                     LLASTSDLKVKNLGKNFLPEININGDAHYQSDVTEIPFSSPAFPIEALDKDQYKISLD
                     VSQMIYDGGMTRKNKDIEYIEHQINRANVGITIYQLKERIVGTYFSIISLQENLKILA
                     INRENLKARLKDVESGIRNGVILASNAEILKAELLKIDQKEIEINAGIDLGYKILSVY
                     TGEEISPGTFLNWANPVVDIHISGNNRLEYSLFGLQAEKTDALKKLASSKLVPRFFAY
                     GSAGYGKPGFNMLLNEFDDYYIIGAKLSWNVWNWNRTSNEKRVFDLQKQVIESNRDAF
                     TRNLSADMEKRISEIAKYEALLPKDREIAGL"
     gene            complement(5386..5994)
                     /locus_tag="PKZ74_09120"
     CDS             complement(5386..5994)
                     /locus_tag="PKZ74_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM92958.1"
                     /translation="MASQYTETERLILEAARKVFLDKGFDGATMQMIATEARINKALL
                     HYYFRSKDRLFEGVFNEAFGKMVPDLMKIFASEAPFDEKIRTFVHAYISALQNYPQIP
                     LFILHEIHRNPGRIIELIRSTGMNPDFVLNVIQNEIKKGTIRDIDPMQLITNMMALCI
                     FPFAARPLIQGFIFNNKEDVYNQFLENRKTEAADFIIKAIKP"
     gene            6112..6513
                     /locus_tag="PKZ74_09125"
     CDS             6112..6513
                     /locus_tag="PKZ74_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92959.1"
                     /translation="MKRNLSAILIIILSIVFIAGCSKTPEQKIAGTWKVEDVKFESVK
                     PVDQQQVEASKKSAQQVSYELFEDKTAKIRAGSTIMEGTWSYKEEEAGVYMSFKDSYQ
                     TSLLGKYEDGKLINIATRPDMTITTIFAKEK"
     gene            complement(6528..7556)
                     /locus_tag="PKZ74_09130"
     CDS             complement(6528..7556)
                     /locus_tag="PKZ74_09130"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPM92960.1"
                     /translation="MKKNPLKLFNYSVVMLMAVISFYSCQFDDPGKKKSIGAVNELLV
                     VTNDKAQWESALGDSIRAVFAAQMPALTQPEPYFDLINVADENFSDLFQKYHNIFIAD
                     INPQAAGVKSETNADVWAVPQRVIRITAPDLSTFYIELARMKATFMEMFIALERQRTL
                     NISQLSVNMDLSREVEQKFGISLPFPGGFYKAGEAPGFMWIRHKVTKARQDVELGIMV
                     YSADYRDQSVFDPRHVVKWRNLVTMQYVPGPSPLSFMKVSEEYIPPVFDTITYFPGGY
                     ALETRGIWEVENDFMGGVFVSYTFINPADNKVMTLDGYVYNPNDDKKNFLRQLEAIFF
                     AMKFSSPN"
     gene            complement(7602..>7878)
                     /locus_tag="PKZ74_09135"
     CDS             complement(7602..>7878)
                     /locus_tag="PKZ74_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPM92961.1"
                     /translation="KKWNNLSSNMIHPNQKLKISGSKPSPANNAGNTAMGTDVTYIYH
                     TVKKGDTLWDIAQQYKGVTVEDIKKWNNLGNSSKLQVGQKLKIAKNG"
BASE COUNT         1875 a         1817 c         1922 g         2164 t
ORIGIN      
        1 cgtcccggtc tctttctctt tcaccaggtt catacccgac ataaacatac cgatgatggt
       61 caccaggagg acaaggatac cggggaccat ataattgata tagttcaatt cgggattgaa
      121 ccagaacgac cgggtggttt tgatcggttc ctccgcctgt atgcccgtag attcgaccag
      181 caggttgatg ttgaaatcct ggataatgga aagggtgtag gcgttcatca gggcagctgc
      241 tgccccgtca atcgcattgg tcacgatctg tacttttgca tttcccgatg tcagcaggtc
      301 tttttcaaac ccttcgggaa tggtcagcac ctggtgggcg cggttcttca gaatatcatc
      361 ctgcgacagt ttctccgaat ggctataggc gacgattttg tagaatgggg atccggagaa
      421 atggcctgcc aggtcgcggg aggttttcgt ccggtcgagg tcagcgacat ggagccgcac
      481 cgttttgatc tcaaaagtgg cggtataggc caggatcagc atctgcacaa aagggatgat
      541 aaagataatc ctgaccatca ccttattccg gaagagctgg atgaattctt tctgcaggat
      601 aaactgaaga gttctcattg tcaggccagc cggattttaa agtttttaac acttaacgtg
      661 atgaagaaca acgtcataaa gacgagaatg agtgtctctt tccatatata ggttatatct
      721 atccctttga gcattatgtt ttttatgatg atgataaacc atttggaagg catgatattg
      781 ctgagggtcc ggagtgccca gggcatattt tcgacgggga aaataaaacc ggagagcagg
      841 atggtcggga gcatcaggcc ggcaagggaa accatcaggg ccacctgctg tgttttcgcg
      901 atggttgaga tcaggatgcc catggataag gccatgatga tgaagagtat ggtttccagg
      961 agcagcagta caatatttcc ccgcatgggc attccgaaga caaagtagcc cagggcgagg
     1021 atggtaaggg cattgatcag cgacagaagg atatagggcg tcactttgcc caggatgatc
     1081 tggagcggtt tgagcggaga gaccagcagg atttccatcg tgcccagctc tttttctttg
     1141 gctatcgaaa tcgaggtcat catggcacag accagcatca gcaaaatagt gatgattccc
     1201 ggtacgaaca taaagacccc tttcagttcc ggattgaaca gcattttggg ttccggggtg
     1261 atgaccatcg ggatggatgc ctggttgagc agcattccgg cctggtaatc tttgatgatg
     1321 gcattggtgt agttagccag aagattggcc gtgttcgggt cggaagcgtc gccaatgatc
     1381 tggataccgg ccgttttatc ttttaccagt ttttctgcaa aattttcctc gaagatgatc
     1441 acttctttga tctttccttt cctgaagagg tcttcgatct gttcttccga ttcgaggtat
     1501 tcatccagga tgaaatatcc tgaggatgtt atcctggaga tgatctcagt ggtaacatca
     1561 tcctttgaat ggtccagaac ggcgatgcgg gcattccgga tctcgttggt cagcgcgaag
     1621 ccgaacagca ggatctggat gaccggcatc ccgaaaagga tgatcatcgt ccggacgtcg
     1681 cggaagatat gaaagaattc tttgatgata aagccgcgca tattgctgtt tatttattgt
     1741 ttcctggcca gcctgataaa cacctcttcc atggattcgg caccgaattg ctttttttgt
     1801 tccgaagggg ttcccaaagc ttcgatcctt ccgtcgacca ttatggaaac cctttcgcag
     1861 tattccgctt cgtccatgta gtgggtagtc aggaagaccg taattccgtc gtgggctgct
     1921 tcatagatca tgtcccagaa ctgccggcgg gtcaatggat cgacgccgct ggtcggttca
     1981 tcgaggaaaa cgatggccgg atcgtgcatg atggcgattg aaaaggccag tttctgtttc
     2041 catcccagcg gcaggtcgct gatcaccctg tccttatatt ccacaatgcc cagcttctcc
     2101 aggaaaaagc tcattttctt cctgatcccg ggcattctca taccataaat tcccccgtag
     2161 aatgtgaagt tttctgcaat cgtcatatct tcatacaggg agaacttctg gctcatataa
     2221 ccgatattcc gtttgatctt ttccgtctct ttatagacat cgaacccggc gacttgaatc
     2281 ctgcccgaag taggtttcca caagccgcac aaaattttga tcgctgttgt ttttccggcg
     2341 ccgttggctc ccaggaaacc gaaaatctcc cctttcttca catagaagtt caggtggtcg
     2401 ttggcaacga acgaaccgaa tttcttgacg aggttctcta cttcgatgat atgatggtcg
     2461 ttagtcattg gtcgcttgtc ctgaaggtga agtcccgaag gatcggtcgt tagtcatcaa
     2521 agccaggaag caatcttcca catcgggaga tatatttctg acttcgatca tttcatgccc
     2581 ttttccggcc aggaaattcc tcaattcatc gccggcattt tcatctttta cagagcaatg
     2641 gataaattct cccgagcggt aggcccggat tgtccggggg aattcctgca catcgaacag
     2701 cagcttgtac attccggaag atctgaccgc cagcagttta tccttatatg caccaacaat
     2761 tccccgggga gtatcgatac ccagaatctt tccctgctgg atcagcgcca cgcggtcgca
     2821 tcggcctgct tcgtccatgt aaggtgttga gaccaggatc gtgatccctt cttttttcag
     2881 ccgggccagc atgtcccaga attctttacg cgaaacggca tccacgccgg ttgtcggttc
     2941 atcgaggaac aggacggccg gtttatggat cagggcgcac gacagggcca gcttttgttt
     3001 catccctccc gacagcttac cggcgggacg gtctttgaac ggttctatct ggttgtaaat
     3061 atccctgatc aggtaatagt tttcctgaat ggtggttttg aagatgctgg caaagaattt
     3121 caggttctct tccacgctca ggtcctggta tagtgaaaac cggcccggca tatagccggc
     3181 aatcttcctg atctgccggt actctttcac cgtgtcgaaa ccttcgacgg tagcctggcc
     3241 gctatccggc agcaaaaggg ttgtcaggat gcggaacaat gttgttttgc ccgatccgtc
     3301 gggcccaatg aagccgaaga gctctccttt atcgaccgac aggctgattg agtcgagggc
     3361 cctgactttt ccgtaggact tactgaggcc ggtgatgcgg accgattcca ttaatggctg
     3421 cagctagtcg gttacgggca tgaaattgat ctcgccgggc atgccgattt tcaggctgcc
     3481 atcgttcggg accagcactt tggcggcata gaccaggttc accctctcct tcttggtctg
     3541 gatggtctta ggtgtgaatt cggctttcgg gctgatccag gaaacgcgtc ccgtgaggtt
     3601 ggtaaagcct gatttgtctt tatcaatcat cacatccaca ttatctccca accggaggta
     3661 aggtagctgg tcgccgctga tatagacttt cagctcaagc gtttccagat tggctacttt
     3721 gcagacaggt tttcccgggg tggtaatttc accggcttcg gcaaatttga ccagtaccgt
     3781 acccgacagc ggtgagacga tgaagcattt gttcatcgat tcttccacct gcctgacctg
     3841 ggcttcgagg gtgcccagtt cggcgcgtac cgattccttc tgcacggcag tggccctgat
     3901 ctgtttgctg atcaggtcga ggtttccgct gatatcgtcc atctgtttct gtgtggcggc
     3961 gccatctttg agcaggttcc cgactctctc cctgtctacc aggaggtttt tcttctgttg
     4021 ttcctgtacg ctgatctgcg aatcaatatt ctgcaaccgg gccaggatca ctttcgactg
     4081 gctcacaagc tgttcgcgct tgaggctcag gtcgatggta tcggtcatag cgactgtatc
     4141 gccgacattc acttgcatac cttcttcaat atttaagtat ttgatttgtc cgttnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagacc ggcaatttcg cggtctttgg
     4321 gcagcaacgc ttcatatttt gcgatctccg atattcgttt ctccatatca gccgaaaggt
     4381 ttcgggtaaa agcatcccgg tttgattcga taacctgttt ctgaagatcg aaaacccgct
     4441 tttcgttgct ggtccggttc cagttccaga cattccagct gagcttagcc ccgatgatat
     4501 aataatcgtc gaattcgttc aacagcatat taaaacccgg tttcccgtag ccggccgaac
     4561 cgtaggcaaa aaaccgcgga acaagcttag atgaagcgag ttttttcaag gcatcggttt
     4621 tctctgcctg gagtccgaaa agcgagtact ccaaccggtt attgccggag atatgaatat
     4681 ctacgaccgg gttagcccaa ttcaggaagg tgccggggga aatctcctct cccgtataga
     4741 ccgacagtat tttatagccc agatcgatgc cggcgttgat ttcgatctct ttctgatcga
     4801 tcttcagcag ttcggcttta aggatttcgg cgttggaggc caggatgacc ccgttccgga
     4861 tgcccgattc cacatctttc agcctggcct tcaggttttc ccggttgatg gccaggatct
     4921 tcaggttttc ctgcaacgaa atgatgctga aatacgttcc gacgatccgt tctttgagct
     4981 ggtagattgt gatcccgaca tttgcccggt tgatctggtg ctcgatgtat tcgatatcct
     5041 tgttctttcg ggtcataccg ccatcataga tcatctggct gacatcgagc gagattttgt
     5101 actggtcttt gtcgagggct tcgataggaa atgcaggaga agagaaggga atttcggtca
     5161 catccgactg gtaatgggca tctccattga tattgatttc cggaaggaag ttcttaccaa
     5221 ggtttttcac tttcaggtcg ctggtggatg ccagcaaatc atattgctgg aacagcggat
     5281 ggctggcttc ggctttcgac aggcaatcgg cgagcgacag ggtgtcggac tgtgcagatg
     5341 cagaagtcag catgaatgta gcgataagga ggagaagagc tgttttcatg gcttgatggc
     5401 tttaatgata aagtcggctg cttcggtctt tctgttttca aggaattgat tataaacatc
     5461 ttccttatta ttaaagatga aaccctggat caggggccgg gctgcgaaag ggaaaatgca
     5521 aagggccatc atattggtga taagctgcat cggatcaata tcccggatgg tgcctttctt
     5581 tatttcattc tggatgacat tcagcacaaa atcggggttc attccggtag aacggatcaa
     5641 ttcaataata cgccccgggt tgcggtggat ttcatgcagg atgaacagcg ggatctgcgg
     5701 atagttttgt aaagcgctga tataggcatg aacgaaggtc ctgatctttt catcaaaagg
     5761 agcttcggag gcgaatattt tcatcaggtc cgggaccatt ttcccgaaag cttcattaaa
     5821 aaccccttca aacagcctgt ctttactgcg gaagtagtaa tgcagcagcg ctttattaat
     5881 ccttgcttcg gtagcgatca tctgcatggt ggccccgtcg aagcctttgt cgaggaacac
     5941 tttccttgct gcttcaagga tcaggcgttc tgtttcggtg tattgagatg ccatctaact
     6001 aatcagttta accattttat ttaaccattt ggtttaatcg atcggacaaa agtaaactat
     6061 attttaattc atgtcaagtg ttttttcata attttgccaa aaaataaaga gatgaaaaga
     6121 aatctttcag ccatccttat catcattctg tcgattgttt ttattgcagg gtgttccaaa
     6181 acaccggagc aaaaaatagc cggtacctgg aaagtggaag atgtcaagtt tgaatcggtc
     6241 aaaccggtag atcagcagca ggtagaagct tcaaagaaaa gcgctcagca ggtttcatac
     6301 gaactctttg aggacaaaac agcgaaaatc cgtgccggtt caacgatcat ggaggggaca
     6361 tggagttata aagaggaaga agccggggtg tatatgtcgt ttaaagattc ttatcagacc
     6421 tccctgcttg ggaaatatga agacggcaaa ctgatcaata ttgccacaag gcctgacatg
     6481 accattacga ccatctttgc caaagaaaaa taggttttaa gttttcttta attcggtgag
     6541 gaaaacttca tcgcgaaaaa gatcgcttcg agctgccgca ggaaattctt cttatcatca
     6601 ttaggattat aaacataccc gtcgagggtc atgactttat tgtcggcagg attaataaaa
     6661 gtatagctga cgaacacgcc tcccataaaa tcgttttcca cctcccagat gccccgggtt
     6721 tctaacgcat aaccccctgg aaaatatgtg atggtatcga aaaccggggg aatatattcc
     6781 tcggagacct tcataaatga caaaggggat ggtcccggga catattgcat agtgaccagg
     6841 tttcgccatt tgaccacatg acgcggatcg aagaccgatt gatcccggta gtcggctgag
     6901 tagaccataa tgccaagttc cacatcctgc ctggctttgg tcactttatg acgaatccac
     6961 atgaatccgg gggcttcgcc ggctttataa aaaccaccgg gaaaaggcaa ggatattccg
     7021 aatttttgtt ccacctcgcg ggaaagatcc atattaaccg ataactggct gatattgagg
     7081 gtccgctgcc tttccaaggc aataaacatt tccatgaatg ttgccttcat ccgggccaat
     7141 tcaatgtaaa aagtggataa atcgggtgcc gtaatcctga taacccgttg aggcactgcc
     7201 cacacatctg catttgtttc cgatttaaca ccggctgcct gtgggttgat gtcagctata
     7261 aaaatgttgt ggtacttctg gaaaaggtct gaaaagtttt cgtcggcgac attgataagg
     7321 tcaaaataag gttcgggctg cgtcaaagcc ggcatctgag ccgcaaaaac ggcccgtatc
     7381 gaatcgccca aagcgctttc ccactgtgct ttatcattgg ttacgactaa aagctcgttc
     7441 acggcgccaa tcgacttctt tttgccggga tcatcaaact ggcaggaata aaaggagata
     7501 acagccatca acattacgac agaatagttg aacagtttca gagggttctt tttcatgaca
     7561 tgaaagcttt aatgtcgaga ttagagatta tttgctgaat atcagccgtt tttcgcgatt
     7621 ttcaacttct gtccgacctg gagttttgat gaatttccga ggttattcca tttttttata
     7681 tcctcaaccg tcactccctt atattgctgg gcaatatccc acagggtatc cccttttttc
     7741 accgtatgat atatataagt aacatcagta cccattgctg tgttgccggc gttattagcc
     7801 ggagatggtt tactgccgga aatcttcaac ttctggttcg ggtggatcat gttcgacgag
     7861 aggttattcc atttcttc
//
LOCUS       DAOPNH010000159         7832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10404_length_7832_cov_7.451074, whole genome shotgun sequence.
ACCESSION   DAOPNH010000159 DAOPNH010000000
VERSION     DAOPNH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10404_length_7832_cov_7.451074"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1147
                     /locus_tag="PKZ74_09140"
     CDS             290..1147
                     /locus_tag="PKZ74_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HPM92962.1"
                     /translation="MEKQNEIKIFEDKKVRTLWDAEQEKWYLSIVDVIAVLTNSPSPQ
                     VYWRVLKMRLKEEGNETVTNCNALKMPAPDGKMRLTDVADTEQLFRLIQSIPSPKAEP
                     FKQWLAQVAAERLDEMQDPELSIDRALEQYMRLGYSENWINQRLKSIEVRKELTDEWK
                     KRGLKEGMQFATLTDIITKAWSDKTTKEYKVLKGLKKENLRDNMTNTELILNMLAEAS
                     TKDISEATKPKDFEESRKVAKQGGNVAKVARKELEAKTGKKVVTALNAKSALQLNEGD
                     KKKPKNKKK"
     gene            complement(1511..2005)
                     /locus_tag="PKZ74_09145"
     CDS             complement(1511..2005)
                     /locus_tag="PKZ74_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HPM92963.1"
                     /translation="MKTKNIITTILIIAGIMSFSACSKDKNIDKEKPVIDISIQDAFP
                     INCDTLYFGESYTLKIRFTDNAELGTYSIEIHHNFEHHSHSTDVEECEHDPEKEPVNP
                     WVFIQDYEIPAGLTEHVTDITLSLPSDCCGEYDEGDYHFFISLTDAEGWSAQKGLSVK
                     IMKR"
     gene            complement(2075..4579)
                     /locus_tag="PKZ74_09150"
     CDS             complement(2075..4579)
                     /locus_tag="PKZ74_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92964.1"
                     /translation="MRKIILLLISINLLHCVSFGQSIYELEGLVVDENNQPLSGATII
                     LFPGEKATTTGFQGNFTIGGLEQGTYRIEISFIGYKPLQDTVTMNGNTTYRAQLSLSP
                     LTLHEVVISDNYSEERKKEEPLNIEIVNDRYLRQNLGGSVMQSLERLPGLSTIDIGPG
                     QSKPVIRGLGFNRVVVLENDIRHEGQQWGADHGLEIDQYAVGRVEVVKGPASILYGSD
                     AIGGVIDLQTNHIPAENTVGGSVDISGKSNNDFGGGSVMLYARKHALHANLRVTLLGF
                     ADYRVPADSIDIYSYRAALDRRRMRNTAGNEQSVNLSVGYLGTKFRSRLFISDLNGKT
                     GFFANAHGLEPRNVDTGLHDASNRDINMPYHYVNHLKIINQSRLDLEKVTVEAGLGFQ
                     RNFIQEWSRYIAHGYMPAAFPGNTGYSADLEREFKKYIYSGNLKIRYDRNDRSEFDFG
                     LNSEFHDNRIGGWAFIIPAYKQFTIGGFALGKYSFSQKSILQAAVRYDYGHIRTAEYR
                     DWYPSPVINESDTAYEHLQRAAAINRDFSSVSWSVGYNYNPGKWSFKANLGKSFRMPI
                     AKELAANGVNYHTFSYEVGDAGLSPEVAWQLDAGAEYGAEKLALGLTPFVNYFRNYIY
                     LNPTAQHDRLYGNGNQVFNYTQARVFRYGFEVHAHYQLIKPLQLGLVIEYVYSEQLSG
                     EKKGFTLPFAPPASGIVNIRYLKPKVGFLRNAYVSLDYRVTATQKHIVPPEEVTKGYQ
                     VVNLGLGADIRMKDQNISLSVQVQNLLNNKYYNHTSYYRLLNVPEPGLNLIIHVSVPF
                     GDRGLNHKGTQRNAQRNTMDYTDNQFLRAPLWFREN"
     gene            complement(4827..5186)
                     /locus_tag="PKZ74_09155"
     CDS             complement(4827..5186)
                     /locus_tag="PKZ74_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92965.1"
                     /translation="MKPFRLYIALILLGIFLFPVLFQSVHSTGHKKKCCSGYENFCES
                     VLPHKDLHTNSLAEQSKEKKCPVCKYQFPVNKLPELSLYSTIAPVITGTICEIILSAD
                     INDAFSIKSPRAPPAIS"
     gene            complement(5183..5599)
                     /locus_tag="PKZ74_09160"
     CDS             complement(5183..5599)
                     /locus_tag="PKZ74_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPM92966.1"
                     /translation="MKASEVLYSRQLKRTSCREGIIDVMMAAGRALSENEIRERLPGN
                     YDRTTFYRSFKTLEEYKIIHKIIIDNQLSKYSLDNSVTHKSEHAHFYCNDCQTVTCLY
                     SIPVQEVRLPNGFTIAEKEILIKGTCPDCKNIQTRI"
     assembly_gap    5641..5740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5870..6292
                     /locus_tag="PKZ74_09165"
     CDS             5870..6292
                     /locus_tag="PKZ74_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPM92967.1"
                     /translation="MTIQDCIKFTNENPICYLATVENDQPRVRIIHFWFADESGFYFQ
                     TGSIKEMYRQLKANPKMEACFYRHEGMLGTMLRIAGEAEFLNDKQMKEKAMADRPFLK
                     EFGLSADSPHLIIFRIAHGQAYFWTKERNLLPKEYIEF"
     gene            6320..7054
                     /locus_tag="PKZ74_09170"
     CDS             6320..7054
                     /locus_tag="PKZ74_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM92968.1"
                     /translation="MKTWKTKSGYTITKVLAGRSNAFLVSNGIVNVMVDTGPARNRKR
                     LQRNLNRLHIEKIDLLLLTHTHYDHAGNAGQIKRTYNATVAVHAREKSFLETGHNILP
                     KGTIPITKLLMNLLGKRLSGRFTYDPCQPDILIEAVRPLNDFGLSACVLPTPGHSPGS
                     VSLIVDDEIAIVGDAMFGIFKGSIFPPFADDTAMMIRSWKVLLDTGCRTFLPAHGFEN
                     SRGKVEKNLRDAIPRDGVPKDGVPAG"
     gene            7139..>7832
                     /locus_tag="PKZ74_09175"
     CDS             7139..>7832
                     /locus_tag="PKZ74_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPM92969.1"
                     /translation="MKNLNKFIFGTVEKVGNALPNPAALFGLFALITLLLSALGYWLN
                     WHGINPSTGETIRVTNLLTHDGLHRILLEMVTNYTSFAPLGIVMVALLGIGIAESSGL
                     VKAAINALILRAPKNSITFMVVFTGVMSNAASDLGYILIIPLAGMIFHSLGRHPVAGM
                     SAAFAGVSGGFSANLVIGTIDPLLAGLSTEAARIIDPEYYVMPTANYYFMAASTFLVS
                     IFGTLVTRYIVEP"
BASE COUNT         1978 a         1776 c         1954 g         2024 t
ORIGIN      
        1 ccccgcttca ccgatttacc ctcgtgctct taatcccgaa taatgttata tttgatctcc
       61 cgacaaaaga attcttgact agcgaaaaag tactggcagc atgagaatga agaggatgtg
      121 gtaatagttc ttcgtggaat ttcgtgaaac cttcgtgggc cttcgtgtaa ctttcaatgt
      181 tcactattac acgaagttac acgaagaaga cacaaagata cacgaagaat tcttagataa
      241 aagctttctt ttagagatct tgtgaagaaa gaaatcgaaa aaaaacgcaa tggaaaagca
      301 aaatgaaatt aaaatttttg aagacaaaaa ggtgagaaca ctttgggatg cagagcagga
      361 aaagtggtat ttgtcgattg ttgatgtgat tgctgttttg actaatagtc ctagtcctca
      421 ggtgtattgg cgtgtattaa aaatgagatt aaaagaagaa ggcaatgaaa ccgttacaaa
      481 ctgtaacgct ttgaaaatgc ctgctcctga cgggaaaatg agattgaccg atgtcgctga
      541 tactgaacaa ctttttcgtc ttattcaatc cattccatct ccaaaagctg aaccctttaa
      601 acaatggctg gcacaggttg ccgcagagcg ccttgacgaa atgcaagacc ctgagctaag
      661 catagacagg gcactggaac aatacatgag acttggatat tcggaaaact ggattaacca
      721 gcgcctaaaa agtattgaag tacgcaaaga attgaccgac gaatggaaaa agagaggatt
      781 gaaagaaggt atgcagtttg ccacgctaac cgatattatt actaaagcat ggagtgataa
      841 aacaaccaaa gaatataaag ttctgaaagg tttgaaaaag gaaaacctgc gggataacat
      901 gactaatacg gaactcatcc tgaatatgct ggccgaagca tccacaaaag acatttcgga
      961 agccacgaaa ccgaaagatt ttgaagaaag caggaaagta gccaaacagg gcggcaatgt
     1021 cgctaaagtt gcacgcaaag aactggaagc caaaacaggc aaaaaggtcg tgaccgctct
     1081 caatgccaaa tccgcgttgc aattgaatga aggtgacaag aaaaaaccga aaaataaaaa
     1141 gaagtagatg ctccaaaaca acgcatagct aaaatccctc atcgacaaac tctggcagaa
     1201 cttccgggga gtattatata ccacctctac cgcctgttaa atctggacga agaggtaaaa
     1261 tgtagtggac attggtagtc ataaggtggt cagaaagtgg tcagaaggtg gtcagaaggt
     1321 agtcataaag ttgtcagaag gttgtcataa gggggtcata agattgtgat tttgaatttg
     1381 agttttggat tttgtttggt gcttggaatt tggaatttgg aatttgtttg gtcccgatta
     1441 ctcgggatgg tgcctggaat ttggaatttg ttttttgctt ggtctgccag ctggcggatg
     1501 gaattttccc tcatctcttc attatcttca cactcagccc tttctgtgcc gaccagcctt
     1561 cggcatcggt caggctgatg aagaaatgat agtcgccttc atcgtactcg ccgcagcagt
     1621 cggatgggag agaaagcgtt atgtcggtca cgtgctcggt tagaccggca ggaatttcat
     1681 agtcctggat aaaaacccag gggttcaccg gctccttctc aggatcatgc tcacattcct
     1741 ccacgtcggt gctgtgcgaa tgatgttcga agttgtgatg tatttcgatg ctgtaggtac
     1801 ccagctctgc attgtcggtg aagcgtattt tcagggtgta ggattcgccg aaataaaggg
     1861 tatcgcaatt gatggggaag gcatcctgga tgcttatgtc gatgacgggc ttctctttgt
     1921 cgatgttctt atccttgctg caggcgctga acgacatgat cccggcgata attaagatgg
     1981 ttgttatgat gttttttgtt ttcatgattc ttattattca atcgttataa acttagtttt
     2041 tcttccgacg agcgtcccgt cggttccaat attgttaatt ttcccggaac cacaaaggcg
     2101 cacgaaggaa ttgattatct gtataatcca ttgtgttcct ttgtgcattc ctttgtgttc
     2161 ctttgtggtt aagtcctctg tctccgaacg gaacggaaac atggatgatc agattcagtc
     2221 ccggctccgg cacattcaac agcctgtaat agctggtgtg attgtaatac ttgttgttca
     2281 gcaggttctg cacctggact gaaaggctta tgttctggtc tttcatccgg atatcagctc
     2341 ccaggccgag gttgaccacc tggtaccctt tggtaacctc ttcgggcggc acaatgtgtt
     2401 tctgggtggc ggtcaccctg taatcgagag atacgtaggc attcctgaga aatccaacct
     2461 ttggtttcag gtacctgata tttactattc ccgaagcggg cggggcaaag ggaagggtaa
     2521 agcccttttt ttcacccgac aactgctccg aataaacata ctcgatgacc agtccgagct
     2581 gcaggggctt aattagctgg taatgggcat gaacttcaaa tccgtaacgg aagacccggg
     2641 cctgggtata gttgaacacc tggttcccgt tgccgtaaag ccggtcgtgc tgagcggtcg
     2701 ggttgaggta gatatagttg cggaaatagt tcacaaaggg ggtgaggccc aatgccagtt
     2761 tctccgcccc gtattcagcg ccggcgtcga gctgccaggc cacttcggga gagaggccgg
     2821 catcgccgac ttcgtagctg aaggtgtggt agttcacccc gttggcggcc agctccttgg
     2881 cgatgggcat ccggaagctc ttgccgagat tggctttaaa cgaccacttc ccggggttgt
     2941 agttatatcc caccgaccac gacacgctgg aaaaatcccg gttgatcgcc gcggcccgct
     3001 gcaggtgctc atacgccgta tcactttcgt ttatcaccgg cgacgggtac cagtcgcggt
     3061 actccgctgt acggatatgg ccgtagtcgt accggaccgc cgcctgcagg atgctcttct
     3121 gcgaaaacga gtactttccg agcgcaaagc caccaatcgt gaattgctta taggcgggga
     3181 tgatgaatgc ccatcccccg atccggttgt cgtggaattc gctgttcagc ccgaaatcga
     3241 attcggatct gtcgttccgg tcgtacctga tcttcaggtt cccggaatag atgtacttct
     3301 taaattcccg ctcgaggtcg gcgctgtagc ctgtgttacc ggggaaagcc gccggcatgt
     3361 agccatgggc aatatatctg ctccattcct gtatgaaatt gcgctgaaag cccaggccgg
     3421 cctccaccgt taccttttcc aggtcaagcc ggctctgatt gatgatcttg agatggttta
     3481 cgtaatgata gggcatgttg atatcgcggt tcgacgcgtc gtgcagtccg gtatcgacat
     3541 tgcgcggttc cagcccgtgc gcgttggcaa agaacccggt ctttccgttc aggtcgctga
     3601 taaagaggcg gctccggaat ttagtcccca ggtaaccgac ggagaggttc acactctgct
     3661 cgttgccggc ggtgttgcgc atacggcgac ggtcgagggc ggcacggtag gagtagatat
     3721 cgatactgtc ggccggtacg cggtagtcgg caaagcccag gagcgtcacg cggaggttgg
     3781 cgtggagcgc atgcttccgt gcgtacagca tgacggatcc accgccgaag tcgttgttgc
     3841 tcttgccgga gatatcaacg ctgcctccga cggtgttttc ggcggggatg tggtttgttt
     3901 gcagatcgat cacaccgccg atggcatccg aaccatacag gatcgacgcg gggcctttca
     3961 ccacctccac ccggccgacg gcatactggt cgatctccag cccgtggtcg gcgccccact
     4021 gctgcccttc gtgccggata tcgttttcca ataccaccac acggttaaag cccaggcccc
     4081 ggatcaccgg tttggattgc cccggtccga tgtcgatcgt ggagaggccc ggcaggcgtt
     4141 cgagcgactg catgacgctg ccgcccaggt tttgcctgag gtaccggtcg ttcacgattt
     4201 cgatgttcag cggctcttcc tttttgcgtt cttcggaata gttatcgctg ataaccactt
     4261 catgcagagt aagagggctt aaggaaagct gtgcccggta agtcgtattc ccgttcatgg
     4321 tcacggtatc ctgcaaaggc ttgtacccga taaaagatat ttcaatccgg taagtaccct
     4381 gctcaaggcc gcctatcgtg aaatttcctt gaaaaccggt cgtggtggct ttttctcccg
     4441 ggaacaggat tatagtggct cctgacaagg gctggttgtt ttcgtcgaca accaagcctt
     4501 ccagttcata aatactttgc ccgaacgaaa cacagtgcaa aagatttatg cttatcagaa
     4561 gcagaattat ttttctcaca ttaaatgaat tgaaaatgaa cttcaactgt aaaaagagga
     4621 tgtttcaaaa agttagattt ctttcaaaat aacttcgtgg atcttcgtgc cttcttcgtg
     4681 atcctctgtg taacaatcat ttataagttc cacgaaggtt tcacgaaggt ttcacgaagg
     4741 tttcacgaag ctacacgaag gggttatgaa acaacctctt agataaaata cccgttccgt
     4801 aaggcgggga tttgaccttg aggagattag ctgatggcag gcggggctct gggtgatttg
     4861 atagaaaaag catcatttat gtctgctgat aaaatgattt cgcagattgt tccggtgata
     4921 accggggcta tcgttgaata aagtgataat tcgggcagtt tattcaccgg gaactggtat
     4981 ttacacaccg ggcatttctt ttcctttgat tgttcagcaa gggaattggt atgcagatcc
     5041 ttatgcggta aaacagactc acagaagttt tcatagcctg aacagcattt ctttttatgg
     5101 ccggttgaat gaaccgattg gaacaggacc gggaaaagaa atattcccag caagatcagg
     5161 gctatataga gtcggaatgg cttcaaatgc gtgtctggat atttttacag tcagggcagg
     5221 tacctttgat caggatctct ttttcggcaa tggtaaagcc gttgggaagc cggacctcct
     5281 ggacggggat ggaatacagg catgtaacgg tctgacaatc attacagtag aaatgagcat
     5341 gttcactttt atgggtcaca gagttatcca gtgagtattt actcaactga ttatcaataa
     5401 ttatcttatg gatgatttta tattcttcca gcgttttaaa ggagcggtag aatgtagtgc
     5461 ggtcgtagtt acccggaagg cgttctctga tttcattttc cgacagggcg cggcctgcgg
     5521 ccatcatcac gtcgatgatc ccttcgcggc agcttgtccg cttcagttgg cgggagtaca
     5581 gaacttcaga agctttcatg gatgcaaata tatttcaaat gcaacaatgt tgcaaagata
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atttattaaa tatcgcctct
     5761 ataatgcggc atcttctctt ttctttattt ttgcctggac ctgaaaaaca tagacaggag
     5821 aagacatcca gtcataaaac attttatatc aatatctaga aaaattatca tgactattca
     5881 ggattgtatt aaattcacca acgagaaccc tatctgctac ttagccactg ttgaaaacga
     5941 ccagccacgc gtccgcatca tccatttctg gtttgccgat gaaagcggtt tttattttca
     6001 gaccggttcg ataaaagaga tgtaccggca actgaaagcc aatcctaaaa tggaagcatg
     6061 cttttacagg cacgagggca tgctcgggac catgcttcgg attgccggcg aggcggagtt
     6121 ccttaacgat aagcagatga aagagaaagc gatggccgac cggccttttc tgaaggaatt
     6181 cggtttatcg gccgacagcc cgcacctgat cattttccgc atcgcccatg gacaagctta
     6241 tttctggacg aaagagcgga acctgttgcc caaggagtac attgagtttt aaaaaaaggg
     6301 acgccatccc tttgaccgga tgaaaacctg gaaaacaaaa agcggataca ccattaccaa
     6361 agttttagcc ggacggagca atgcttttct tgtttcaaac ggtattgtca atgtaatggt
     6421 cgataccggc ccggcaagaa accggaaaag gctgcaaaga aatttaaatc ggcttcacat
     6481 agaaaagatt gatttacttc ttcttaccca tactcattat gaccatgcgg ggaatgcagg
     6541 ccagatcaaa agaacttata atgcaactgt agcggtgcat gcccgggaaa agtcttttct
     6601 tgaaaccggc cataacatcc tgccaaaagg gaccattccg attacaaaat tattgatgaa
     6661 ccttctgggt aaacggttat ccggaagatt cacatatgac ccttgtcagc ctgatatttt
     6721 gattgaggcc gttcgtccgc tgaacgattt tggtttgagt gcctgtgttc tgcccactcc
     6781 gggacattcc cccggatcgg tgagccttat tgtggatgat gagatcgcca ttgtgggcga
     6841 cgccatgttc ggaatattca aaggctcaat ctttcctccc tttgccgatg atacggctat
     6901 gatgatcagg agctggaaag tgttgctgga taccggatgt cgcacctttc tgccggcgca
     6961 tggctttgaa aacagccggg ggaaagtgga gaagaactta agggacgcca tcccgaggga
     7021 tggcgtcccg aaggatggcg tccctgcggg ctaaacaaaa aggcggaaag agtgttaatt
     7081 ctttctataa acagattaac attctaaaga tcttaccctc aatcagtcct gatgcacgat
     7141 gaagaacctc aacaaattca ttttcggtac ggtggagaag gttggcaatg cattgccgaa
     7201 ccctgctgca ttgttcggcc tgtttgcttt aatcaccctg ctgttatcgg ctttagggta
     7261 ctggttgaac tggcacggca tcaatccatc caccggtgaa acgatccgtg ttaccaacct
     7321 ccttacccat gacgggttgc accggatcct gctggagatg gtgaccaatt atacaagctt
     7381 tgccccgctg ggcatcgtga tggtggcttt gctggggatc ggtattgccg agagcagcgg
     7441 actagtgaag gcagctatta atgccttgat cctgagggct cccaaaaatt ccatcacctt
     7501 tatggtcgta tttaccggtg ttatgtcgaa tgccgcttcc gacctgggct acatcctcat
     7561 catcccactg gccgggatga tctttcattc cctgggccgg cacccggtag caggcatgtc
     7621 ggcagctttt gccggggtca gcggaggatt cagcgccaac ctggtgatcg gcaccatcga
     7681 tccgctcctg gccggcctct ccacggaagc agcccgtatc atcgacccgg agtattacgt
     7741 gatgccgacg gccaactact atttcatggc ggcgtcgaca ttcctggtct ccattttcgg
     7801 cacactggtc acccgctata ttgtagaacc gc
//
LOCUS       DAOPNH010000160         7804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10450_length_7804_cov_6.391534, whole genome shotgun sequence.
ACCESSION   DAOPNH010000160 DAOPNH010000000
VERSION     DAOPNH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10450_length_7804_cov_6.391534"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKZ74_09180"
     CDS             <1..659
                     /locus_tag="PKZ74_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPM92970.1"
                     /translation="CVTAQEWPRYSKELVDAGADGIELNVFIVPKNEQMTSAEIEDLY
                     VQIVSEVRRNVNVPIAVKIGPFFSNGFSMVKRLNDAGANAIVVFNRFYRPDIDIDNEC
                     ITRPNYLSCPQEMGQPLRWVSLFSSRVNIDMAGNTGIHEGSGMIKMLLAGAAAVQVCS
                     TLYINGLGYIDTMIGDLKKWMAWKGYEKIDDFKGKLTKRHENIAAFERVQFLKQALGE
                     "
     gene            complement(666..1562)
                     /locus_tag="PKZ74_09185"
     CDS             complement(666..1562)
                     /locus_tag="PKZ74_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPM92971.1"
                     /translation="MKMNYIFILLILMTSCASSNVPQHKTGFDELILAGQDVFFKDVT
                     FENDIDFTKFERNLISEGVYQVRIVSSVTFQNCIFKGKVITYKVDEDQNITLTSFQSN
                     VTFIGCNFNEEANFRASAILGRCDFTNSLFLKGASFEECTFFQNAYFRGSAYHEEARF
                     QNAVFMQKANFLNAEFDVTASFQSATFNSEAQFSSAKFLGYADFGLTRFNGNFFSNYA
                     TFGKQVIFNHATFYGQADMTSMTLKRSEMKNCRFFNEARFAKSIVEESLTLSNTFFLF
                     GKPDLSSFDQQKVIQDGLRTGE"
     gene            complement(1639..2379)
                     /locus_tag="PKZ74_09190"
     CDS             complement(1639..2379)
                     /locus_tag="PKZ74_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92972.1"
                     /translation="MEPPDDIDPNVTGVDLIPWVFDDYSTFSWDYPTERIIQFNIYNA
                     GNADAPSSADWAYYYIYYNAFDADDYGVIFYDQFNQSVPENTYDCPNDWNCIFNIPIP
                     AGNDFAWYAWESYWVERYYYMPEITGFYYLLCIADAEDKFQEQDELNNLFYTTIDPVY
                     FEYGVGKKSSQKFYNPFTFKNDVEFNKEKSRRTKYNSAVTQDFRNAYTPEEIIEFFKK
                     EKISGRLDGKVDEYVQRNAQLNASKQLK"
     gene            complement(2346..3290)
                     /locus_tag="PKZ74_09195"
     CDS             complement(2346..3290)
                     /locus_tag="PKZ74_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM92973.1"
                     /translation="MKIFRFLMALAIIAGITLAIGCKKDKDDDPDDNPQYALGWFGTD
                     DPSKVPSSTNFGFGGGNVPPDYSLVDKFPPIGDQGQYGTCVAWAVGYNTKTMVNGLSK
                     GYGASDLAQANKQFSPRDLFTAIDDANKGANCGGTNFNFALDLVQNRGIATLATAPYT
                     TNLGTCEQATLDPAWTQEAGNYKFKFWRKIDASIEAIKENISQNVPVVFGARLADNFM
                     SHNTDEVISSATSYDNVGQHAYHAMVIAGYDDNKGPNGALRCINSWGTSWGGNGYFWV
                     DYNFFMNNFVSTSAMKRTFTLQPMPTVMWNLRTISIRT"
     gene            complement(3414..3971)
                     /locus_tag="PKZ74_09200"
     CDS             complement(3414..3971)
                     /locus_tag="PKZ74_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92974.1"
                     /translation="MRLCLIIRIRIALIISRHIINAGAGIDYMSGNDQSNTDADYQAK
                     SHAFDLLYGIRHRYYGHMDLFSDFPKATANGGLVDAIIRLKYFVTEKANIGADVHFFS
                     LQNNVIDKSSKALAYYSKGLGQEVDLNFSWDVNKIFNVKAGYSVFLLSETMEKIQGVH
                     NNSRFPNWAWIMITAKPVFLDTSVK"
     gene            4017..4391
                     /locus_tag="PKZ74_09205"
     CDS             4017..4391
                     /locus_tag="PKZ74_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92975.1"
                     /translation="MDLFSDFPKATANGGLVDAIIRLKYFVTEKANIGADVHFFSLQN
                     NVIDKSSKALAYYSKGLGQEVDLNFSWDVNKIFNVKAGYSVFLLSETMEKIQGVHNNS
                     RFPNWAWIMITAKPVFLDTSVK"
     gene            4515..5459
                     /locus_tag="PKZ74_09210"
     CDS             4515..5459
                     /locus_tag="PKZ74_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM92976.1"
                     /translation="MKIFRFLMALAIIAGITLAIGCKKDKDDDPDDNPQYALGWFGTD
                     DPSKVPSSTNFGFGGGNVPPDYSLVDKFPPIGDQGQYGTCVAWAVGYNTKTMVNGLSK
                     GYGASDLAQANKQFSPRDLFTAIDDANKGANCGGTNFNFALDLVQNRGIATLATAPYT
                     TNLGTCEQATLDPAWTQEAGNYKFKFWRKIDASIEAIKENISQNVPVVFGARLADNFM
                     SHNTDEVISSATSYDNVGQHAYHAMVIAGYDDNKGPNGALRCINSWGTSWGGNGYFWV
                     DYNFFMNNFVSTSAMKRTFTLQPMPTVMWNLRTISIRT"
     gene            5426..6166
                     /locus_tag="PKZ74_09215"
     CDS             5426..6166
                     /locus_tag="PKZ74_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92977.1"
                     /translation="MEPPDDIDPNVTGVDLIPWVFDDYSTFSWDYPTERIIQFNIYNA
                     GNADAPSSADWAYYYIYYNAFDADDYGVIFYDQFNQSVPENTYDCPNDWNCIFNIPIP
                     AGNDFAWYAWESYWVERYYYMPEITGFYYLLCIADAEDKFQEQDELNNLFYTTIDPVY
                     FEYGVGKKSSQKFYNPFTFKNDVEFNKEKSRRTKYNSAVTQDFRNAYTPEEIIEFFKK
                     EKISGRLDGKVDEYVQRNAQLNASKQLK"
     gene            6243..7139
                     /locus_tag="PKZ74_09220"
     CDS             6243..7139
                     /locus_tag="PKZ74_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPM92978.1"
                     /translation="MKMNYIFILLILMTSCASSNVPQHKTGFDELILAGQDVFFKDVT
                     FENDIDFTKFERNLISEGVYQVRIVSSVTFQNCIFKGKVITYKVDEDQNITLTSFQSN
                     VTFIGCNFNEEANFRASAILGRCDFTNSLFLKGASFEECTFFQNAYFRGSAYHEEARF
                     QNAVFMQKANFLNAEFDVTASFQSATFNSEAQFSSAKFLGYADFGLTRFNGNFFSNYA
                     TFGKQVIFNHATFYGQADMTSMTLKRSEMKNCRFFNEARFAKSIVEESLTLSNTFFLF
                     GKPDLSSFDQQKVIQDGLRTGE"
     gene            complement(7146..>7804)
                     /locus_tag="PKZ74_09225"
     CDS             complement(7146..>7804)
                     /locus_tag="PKZ74_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPM92979.1"
                     /translation="CVTAQEWPRYSKELVDAGADGIELNVFIVPKNEQMTSAEIEDLY
                     VQIVSEVRRNVNVPIAVKIGPFFSNGFSMVKRLNDAGANAIVVFNRFYRPDIDIDNEC
                     ITRPNYLSCPQEMGQPLRWVSLFSSRVNIDMAGNTGIHEGSGMIKMLLAGAAAVQVCS
                     TLYINGLGYIDTMIGDLKKWMAWKGYEKIDDFKGKLTKRHENIAAFERVQFLKQALGE
                     "
BASE COUNT         2150 a         1752 c         1752 g         2150 t
ORIGIN      
        1 actgtgttac ggcacaggag tggccgcgct attcgaaaga actggttgac gcaggagcag
       61 atggcattga actcaatgtt ttcatcgttc cgaaaaacga gcagatgaca agcgctgaaa
      121 ttgaagacct ttatgtacag atcgtatcgg aagtccggcg taatgtcaat gttccgattg
      181 cggtgaagat cggaccattc ttcagtaatg gtttcagcat ggtcaaaagg ttaaacgatg
      241 cgggtgccaa tgcaatagtc gtctttaacc gtttttaccg gcctgatatt gatattgata
      301 atgaatgtat taccagacca aattacctga gctgcccaca ggaaatgggc cagcctttgc
      361 gttgggtatc cctgttttcc agccgtgtta atattgatat ggcaggtaat acgggaatac
      421 acgaaggatc gggaatgatc aaaatgctgc tggccggcgc agccgccgtc caggtttgtt
      481 caaccttgta tatcaacggc ctgggctata tcgatacgat gatcggagac cttaaaaaat
      541 ggatggcctg gaaaggatac gaaaaaatcg atgattttaa aggaaaactg accaaacgcc
      601 atgaaaatat tgcagcgttc gaacgtgtcc agttcctgaa acaggccctc ggagaataag
      661 cggctttact cgccggttct caggccatcc tgaataactt tttgctgatc gaaactgctc
      721 aggtcaggtt ttccgaataa aaagaaggta ttggaaagcg tcaggctttc ttcaacaatt
      781 gatttggcaa acctggcttc gttgaaaaac ctgcagttct tcatctcact tctcttcagg
      841 gtcattgatg tcatatcagc ctggccgtaa aaggtggcat ggttgaaaat aacctgtttg
      901 ccgaaagtgg cgtaattgga gaagaaattt ccgttgaacc gggtcaggcc gaaatcggcg
      961 tatcccagga attttgcgga actgaactgc gcttcactgt tgaatgtcgc gctttgaaaa
     1021 ctggcggtca catcaaattc cgcattcagg aaattggcct tctgcataaa gactgcattc
     1081 tggaaacggg cctcttcatg atatgcgctc cccctgaaat aagcattctg aaaaaatgtg
     1141 cattcctcaa agctggcccc ttttaagaag agggaattcg taaaatcaca acggcccaga
     1201 atggctgaag cacgaaaatt agcttcttca ttaaaattac acccgatgaa tgtcacattg
     1261 ctctgaaatg atgttaatgt aatattttga tcttcatcta ctttataggt gattactttc
     1321 cctttgaaaa tgcagttctg gaaagtaacc gatgaaacga tgcggacctg gtacacccct
     1381 tcgctgatca ggtttcgttc gaactttgta aagtcgatgt cattttcaaa agtaacgtct
     1441 ttgaagaaca catcctgccc tgccaggatg agttcgtcga aaccggtttt atgctgcggt
     1501 acattgctgg aagcacagga agtcatcaaa atcaataaaa taaaaatgta attcatcttc
     1561 ataatataaa taattaaaaa aggtgtgcaa agggcctgaa ttgcccggat tgcacacctt
     1621 atcagttttt ttccaaattt atttcaattg tttgctggca ttcagctggg catttctctg
     1681 cacgtattca tccactttgc cgtcaagcct gccgctgatt ttttccttct tgaaaaattc
     1741 aatgatctct tccggagtgt aggcattcct gaaatcttga gttacagcag aattgtattt
     1801 ggttctgcgt gatttttcct tattgaattc aacgtcattc ttaaaagtga aaggattata
     1861 gaacttctgc gaagactttt tcccgacgcc gtattcgaaa taaaccggat cgatcgtggt
     1921 gtagaacaga ttgttgagtt catcctgctc ctggaattta tcttctgcat cggcaatgca
     1981 gagcaggtaa tagaaaccag tgatctcggg catataataa tatctttcta cccagtaaga
     2041 ttcccaggca taccaggcaa agtcatttcc tgccgggatg ggaatattga agatgcagtt
     2101 ccagtcgttc gggcaatcgt aggtgttttc gggcaccgac tggttgaact ggtcatagaa
     2161 tatcaccccg taatcatcag cgtcgaatgc gttgtagtag atatagtaat aagcccagtc
     2221 ggccgatgat ggagcatctg cgttgccggc attgtagata ttgaactgga taattctctc
     2281 ggtcgggtaa tcccagctaa aggtggaata atcatcgaat acccagggga tgagatcgac
     2341 gccggtcacg ttcggatcga tatcgtccgg aggttccaca tcactgtcgg cattggctgc
     2401 aatgtaaagg ttcgtttcat tgctgaggtg gagacaaaat tgttcataaa gaagttgtag
     2461 tcgacccaga agtagccatt accgccccag gaggtgcccc atgaattgat gcagcgaagc
     2521 gccccattcg gccctttgtt gtcgtcgtat cctgcaatga ccatggcatg gtaagcatgc
     2581 tggcccacat tgtcgtaaga agtagcggaa gagatcactt catcggtgtt gtgcgacata
     2641 aaattgtcag ccagccgtgc accgaagact acgggcacat tttgcgaaat attctcctta
     2701 atggcttcga tagaggcatc gatctttctc cagaatttga acttgtagtt gccagcttcc
     2761 tgtgtccatg ccggatcaag ggtggcctgc tcgcatgtgc ccaggttggt ggtataggga
     2821 gctgtcgcta aagtcgcgat acccctgttc tgcaccagat ccagggcgaa attgaagttg
     2881 gtcccgccgc aattggcccc tttattggca tcatcaattg cagtaaagag gtcacgcggg
     2941 ctgaactgct tgttggcttg tgccagatcg cttgcgccat atcccttcga aagaccgttt
     3001 accatagttt ttgtgttgta accgaccgcc caggcaacac aggtcccata ctgaccctgg
     3061 tctccgatgg gcgggaattt gtcgacaagc gaataatcgg gagggacatt tccgccaccg
     3121 aacccgaaat tagtggaaga cgggaccttg ctggggtcat ctgtcccgaa ccaaccgagc
     3181 gcatattgtg ggttgtcatc gggatcatcg tctttgtctt ttttacatcc tatcgccagg
     3241 gttatccctg cgataatggc caatgccatg agaaacctga aaattttcat aaatgaaaag
     3301 atttaatgaa gaggtttgaa attgggttaa ttattaatgt ttttgatatt attatctcaa
     3361 aaatataaat aatctaaata tggaaatcat aacgaaatgt taaattctgg aaattatttc
     3421 acagaagtgt ccaaaaatac cggtttggct gtaatcatga tccaggccca gttcggaaaa
     3481 cgggaattgt tgtgaacgcc ctggattttc tccatcgttt ccgaaagcag aaataccgag
     3541 tacccggcct tgacattgaa gattttattc acatcccacg agaagttcag atcgacttcc
     3601 tgtccgagac cttttgagta ataggcaagc gccttagaag acttatcaat gacattgttt
     3661 tgtaaactga aaaaatgaac atcggctccg atattagctt tctccgtgac aaaatacttc
     3721 agacggatga tggcatctac caggccgcca ttggctgtgg ctttgggaaa atccgaaaac
     3781 aaatccatat ggccataata gcggtgacga ataccgtata aaagatcgaa tgcgtgagac
     3841 tttgcctgat aatccgcatc cgtattgctt tgatcatttc ccgacatata atcaatgccg
     3901 gcgccggcat tgattatatg tcgggaaatg atcaaagcaa tacggatgcg gattatcagg
     3961 caaagtctca cgcattcgat cttttatacg gtattcgtca ccgctattat ggccatatgg
     4021 atttgttttc ggattttccc aaagccacag ccaatggcgg cctggtagat gccatcatcc
     4081 gtctgaagta ttttgtcacg gagaaagcta atatcggagc cgatgttcat tttttcagtt
     4141 tacaaaacaa tgtcattgat aagtcttcta aggcgcttgc ctattactca aaaggtctcg
     4201 gacaggaagt cgatctgaac ttctcgtggg atgtgaataa aatcttcaat gtcaaggccg
     4261 ggtactcggt atttctgctt tcggaaacga tggagaaaat ccagggcgtt cacaacaatt
     4321 cccgttttcc gaactgggcc tggatcatga ttacagccaa accggtattt ttggacactt
     4381 ctgtgaaata atttccagaa tttaacattt cgttatgatt tccatattta gattatttat
     4441 atttttgaga taataatatc aaaaacatta ataattaacc caatttcaaa cctcttcatt
     4501 aaatcttttc atttatgaaa attttcaggt ttctcatggc attggccatt atcgcaggga
     4561 taaccctggc gataggatgt aaaaaagaca aagacgatga tcccgatgac aacccacaat
     4621 atgcgctcgg ttggttcggg acagatgacc ccagcaaggt cccgtcttcc actaatttcg
     4681 ggttcggtgg cggaaatgtc cctcccgatt attcgcttgt cgacaaattc ccgcccatcg
     4741 gagaccaggg tcagtatggg acctgtgttg cctgggcggt cggttacaac acaaaaacta
     4801 tggtaaacgg tctttcgaag ggatatggcg caagcgatct ggcacaagcc aacaagcagt
     4861 tcagcccgcg tgacctcttt actgcaattg atgatgccaa taaaggggcc aattgcggcg
     4921 ggaccaactt caatttcgcc ctggatctgg tgcagaacag gggtatcgcg actttagcga
     4981 cagctcccta taccaccaac ctgggcacat gcgagcaggc cacccttgat ccggcatgga
     5041 cacaggaagc tggcaactac aagttcaaat tctggagaaa gatcgatgcc tctatcgaag
     5101 ccattaagga gaatatttcg caaaatgtgc ccgtagtctt cggtgcacgg ctggctgaca
     5161 attttatgtc gcacaacacc gatgaagtga tctcttccgc tacttcttac gacaatgtgg
     5221 gccagcatgc ttaccatgcc atggtcattg caggatacga cgacaacaaa gggccgaatg
     5281 gggcgcttcg ctgcatcaat tcatggggca cctcctgggg cggtaatggc tacttctggg
     5341 tcgactacaa cttctttatg aacaattttg tctccacctc agcaatgaaa cgaaccttta
     5401 cattgcagcc aatgccgaca gtgatgtgga acctccggac gatatcgatc cgaacgtgac
     5461 cggcgtcgat ctcatcccct gggtattcga tgattattcc acctttagct gggattaccc
     5521 gaccgagaga attatccagt tcaatatcta caatgccggc aacgcagatg ctccatcatc
     5581 ggccgactgg gcttattact atatctacta caacgcattc gacgctgatg attacggggt
     5641 gatattctat gaccagttca accagtcggt gcccgaaaac acctacgatt gcccgaacga
     5701 ctggaactgc atcttcaata ttcccatccc ggcaggaaat gactttgcct ggtatgcctg
     5761 ggaatcttac tgggtagaaa gatattatta tatgcccgag atcactggtt tctattacct
     5821 gctctgcatt gccgatgcag aagataaatt ccaggagcag gatgaactca acaatctgtt
     5881 ctacaccacg atcgatccgg tttatttcga atacggcgtc gggaaaaagt cttcgcagaa
     5941 gttctataat cctttcactt ttaagaatga cgttgaattc aataaggaaa aatcacgcag
     6001 aaccaaatac aattctgctg taactcaaga tttcaggaat gcctacactc cggaagagat
     6061 cattgaattt ttcaagaagg aaaaaatcag cggcaggctt gacggcaaag tggatgaata
     6121 cgtgcagaga aatgcccagc tgaatgccag caaacaattg aaataaattt ggaaaaaaac
     6181 tgataaggtg tgcaatccgg gcaattcagg ccctttgcac acctttttta attatttata
     6241 ttatgaagat gaattacatt tttattttat tgattttgat gacttcctgt gcttccagca
     6301 atgtaccgca gcataaaacc ggtttcgacg aactcatcct ggcagggcag gatgtgttct
     6361 tcaaagacgt tacttttgaa aatgacatcg actttacaaa gttcgaacga aacctgatca
     6421 gcgaaggggt gtaccaggtc cgcatcgttt catcggttac tttccagaac tgcattttca
     6481 aagggaaagt aatcacctat aaagtagatg aagatcaaaa tattacatta acatcatttc
     6541 agagcaatgt gacattcatc gggtgtaatt ttaatgaaga agctaatttt cgtgcttcag
     6601 ccattctggg ccgttgtgat tttacgaatt ccctcttctt aaaaggggcc agctttgagg
     6661 aatgcacatt ttttcagaat gcttatttca gggggagcgc atatcatgaa gaggcccgtt
     6721 tccagaatgc agtctttatg cagaaggcca atttcctgaa tgcggaattt gatgtgaccg
     6781 ccagttttca aagcgcgaca ttcaacagtg aagcgcagtt cagttccgca aaattcctgg
     6841 gatacgccga tttcggcctg acccggttca acggaaattt cttctccaat tacgccactt
     6901 tcggcaaaca ggttattttc aaccatgcca ccttttacgg ccaggctgat atgacatcaa
     6961 tgaccctgaa gagaagtgag atgaagaact gcaggttttt caacgaagcc aggtttgcca
     7021 aatcaattgt tgaagaaagc ctgacgcttt ccaatacctt ctttttattc ggaaaacctg
     7081 acctgagcag tttcgatcag caaaaagtta ttcaggatgg cctgagaacc ggcgagtaaa
     7141 gccgcttatt ctccgagggc ctgtttcagg aactggacac gttcgaacgc tgcaatattt
     7201 tcatggcgtt tggtcagttt tcctttaaaa tcatcgattt tttcgtatcc tttccaggcc
     7261 atccattttt taaggtctcc gatcatcgta tcgatatagc ccaggccgtt gatatacaag
     7321 gttgaacaaa cctggacggc ggctgcgccg gccagcagca ttttgatcat tcccgatcct
     7381 tcgtgtattc ccgtattacc tgccatatca atattaacac ggctggaaaa cagggatacc
     7441 caacgcaaag gctggcccat ttcctgtggg cagctcaggt aatttggtct ggtaatacat
     7501 tcattatcaa tatcaatatc aggccggtaa aaacggttaa agacgactat tgcattggca
     7561 cccgcatcgt ttaacctttt gaccatgctg aaaccattac tgaagaatgg tccgatcttc
     7621 accgcaatcg gaacattgac attacgccgg acttccgata cgatctgtac ataaaggtct
     7681 tcaatttcag cgcttgtcat ctgctcgttt ttcggaacga tgaaaacatt gagttcaatg
     7741 ccatctgctc ctgcgtcaac cagttctttc gaatagcgcg gccactcctg tgccgtaaca
     7801 cagt
//
LOCUS       DAOPNH010000161         7795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10468_length_7795_cov_7.091602, whole genome shotgun sequence.
ACCESSION   DAOPNH010000161 DAOPNH010000000
VERSION     DAOPNH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10468_length_7795_cov_7.091602"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..421
                     /locus_tag="PKZ74_09230"
     CDS             200..421
                     /locus_tag="PKZ74_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HPM92980.1"
                     /translation="MTRSELEAILKEGVNSCANPQGWANLAELGTYLRQRGLQYGKLS
                     RFLQDYQNLIETRMDTNITPPAVYARLRQ"
     gene            complement(473..1846)
                     /locus_tag="PKZ74_09235"
     CDS             complement(473..1846)
                     /locus_tag="PKZ74_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM92981.1"
                     /translation="MFAKGVNLPKKSVTLKTSVTNKDIWLIAYPIILGSVAQNIINIT
                     DTAFLGRVGQVALGAGAIGGIFYLVASMLAWGFGIGSQIIIARRHGEQAFGEIGRTFR
                     HGFFFMLPLTLTLFSLMKFFSGDLLSLIISSEAVYDSLQEYLGYRSWGIFFAGVNMLY
                     RGLYIGIARTKVITYTTFILAFTNAFLDYCLIFGNLGFPELGIGGAALASTLSEAVAT
                     VYFTVYTFFILDGRKYRITARSRFDEQLYRRIIRISLPVMMQHFISMVAWLVFFLFIE
                     KLGEMALAVSNIVRSFYVILMIPMWGFSSATSTLVSSLIGQGRQGEVMSLVYKVARMC
                     AFGVMIVAALGALFPDYALMIYTDDAGMIKASLPALYVVNISVLFLAVGFVFFSAVSG
                     TGKTQVSFLIELAVIVFYLVFSYLLAVVWRAEVYVVWMAEFFYAFMLGTLSYLYLKRG
                     KWKSVRI"
     assembly_gap    2061..2160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2292..3215
                     /locus_tag="PKZ74_09240"
     CDS             2292..3215
                     /locus_tag="PKZ74_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPM92982.1"
                     /translation="MKTLIIGATGFIGRELVKDLADHGHTVVAVTRNSGMARAILGKK
                     CSIVEWDGISVSSLVHYMAGCEAVVNLAGENIASGRWTEKRKEALVASRTTVGRKLAE
                     AILRLTSPLPVLVQGSAIGIYGTDVDSASDESLAPGSGFLANLVVEWENSVAEALPNV
                     KRLAWARTGLVLGRDGGLLEKMLLPFRFHSGTLLGSGRQWMSWIHLSDEVRAIRFLLE
                     NNTSSGHYNLTAPMPVKMEEFIRALSRITGKPAWVRVPGAVIKALMGEMAAETVLASQ
                     NALPARLLRDGFTFDYPRLEDALTNLLTNNE"
     gene            3208..4518
                     /locus_tag="PKZ74_09245"
     CDS             3208..4518
                     /locus_tag="PKZ74_09245"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPM92983.1"
                     /translation="MNNTEINLFWFRRDLRLDDNAGLFHVLSSGLPVLPIFIFDDDIL
                     LKLDNKEDARVTFIYDEVLRIKQEIESRGGSLLIKRGRPAGIFRELTGRFRISKVFAN
                     HDYEPYARNRDDRVGKLLSEQGIRFITFKDQVIFEKDEILKDDGKSYTVFTPYSRRWK
                     SALSGSSFTKHNSAGLTGNFLKMMPMDVPALSSLGFNRSRLTFPPREPDAGILQHYHR
                     DRDFPALQGTSRMGPHLRFGTVSIRKLAEMAHKTNEVFLNELIWREFYMMILWHFPHA
                     AERAFKPQYDRIAWRNDEQQFRAWCEGRTGYPIVDAGMRELNATGYMHNRVRMITASF
                     LARHLLIDWRWGDAWFASKLLDFELSSNNGGWQWAAGTGCDAAPYFRVFNPVLQAEKF
                     DPEKNYIRQWVPEYGTGSYPPPIVEHSRARLLAIETYQKALKPD"
     gene            4582..6972
                     /locus_tag="PKZ74_09250"
     CDS             4582..6972
                     /locus_tag="PKZ74_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM92984.1"
                     /translation="MKRKIISSLSVIFALIPFMIHAQQGSVKGRIFDESNNEPIAFAN
                     IIVFGTTIGSTSDLDGNFIFTGLEPGFIKLAASAVGYESKVTIDFQVTNSKTSFMDIP
                     MKPKEFELQEVVIKASPFKRLEESPVSLRTLQISEIEKNPGGNRDISRVIQVLPGVSS
                     TPAFRNDVLVRGGGGNENAFYLDGIEIPNLNHFSTQGSSGGPVGIINADFIREVDFYS
                     GAFPADRGKSLSSLLDMKLIDGNKEEWIFRGSLGASDLALTTNGPVSDNSTFLFSYRR
                     SYLQFLFDALGLPFLPNYDDFLAKYKIQFDQKNELSVIGLGSIDRFELNTGIKDPDES
                     QAYILGYLPVQEQWSYTIGAVYKHYHKNGFTDVILSRNMLDNRSFKYRNNDENDTLNF
                     NYNSQESENKLRVEHTSRRGAFKYTYGGGLELARYTNSTFQRLYANDVYTPVNYDSQL
                     RMFLWNVFGQVSKNFFDDRLVLSLGLRMDANDYTPYMANLLNQVSPRFSASFGLTDIW
                     FLNFNTGRYYQRLPYTTFGFRDNNGELVNKRDDLRNLSADHVVFGFEVLPERDAKFSI
                     EGFYKYYRNYPFSVRDSINIASKSAGYGTFGDEQVISVSDGRAYGFEVLAQDKSLFGF
                     NVILSYTFVVSKFTDKDDKFVPSAWDNRNILNLTVLKSFKRNWDIGAKWRYVGGAPYT
                     PADLEFSSYVPAWDARGREYPDNSRFNELRLKAFHQLDLRVDKTYFFKKWSLNLYIDV
                     QNVYNFQAENPPNYTNLDKDGNKVYQDPPTNQFYQLRALNSESGTVLPTLGIIVEF"
     gene            6987..7748
                     /locus_tag="PKZ74_09255"
     CDS             6987..7748
                     /locus_tag="PKZ74_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92985.1"
                     /translation="MKTRIFSIAFIFFLGGLFIVGTSRCSTARKTADSGQPEIINTNI
                     NGQGLSIRINFTKGESHNHPLMAFWLEDKDGKYIETIYISESIGKGIFQHGDRSKGRW
                     QPGEVRRPAALPYWGHKRGIQAPDGYFIPTVALPMPDAVTGPTPKGDFILKSKSTEQA
                     PRIFKVMMEINQSWDWNEFWTNSKYQGDVNYMTSSQPSLVYEAVINLDSPVKEYEMKP
                     VGHGHYSGADGSLNPDLSTITTAGEIAESVKVTVE"
BASE COUNT         1925 a         2072 c         2008 g         1690 t
ORIGIN      
        1 gatgtgtcgg gttatccgcc ggggatgtat gtggtggtgg taacggatag gaaagagagg
       61 aggtattcgg gaaagatggt tatcaggtaa ttagccgatc cgacaaaatc gggaaatcat
      121 acaaaaaacc ctttactttt gaaccaattt gttgtatatt tgtttttctc gttaagaaac
      181 agttaaaact ttttttaaaa tgacacgctc cgaattagaa gctattttaa aggaaggggt
      241 taacagctgc gccaacccgc aaggttgggc gaacctggcg gaattaggaa cctatttgcg
      301 ccagcgagga cttcaatacg gcaaattgtc acgcttcctg caagactacc agaacctcat
      361 cgagacccgg atggacacca acatcactcc ccctgccgta tacgcccggc ttcgccagta
      421 atgctgcctg cctgctatcc tgccaccttc gggtgaatat cttttctgac ggtcaaatcc
      481 ttacggattt ccatttgcct cttttcaggt agagatacga taatgtaccc agcataaaag
      541 catagaagaa ctcggccatc cagaccacgt aaacctccgc cctccaaacc acggccagca
      601 aataggaaaa taccaggtaa aatacgatca cggccagttc gatcaggaac gacacctgcg
      661 ttttgcccgt tcccgatacg gcgctgaaaa atacaaaccc gacagccagg aaaaggaccg
      721 atatattcac tacgtacaaa gccggtagtg aagctttgat cattcccgca tcgtcggtgt
      781 agatcatcag ggcgtaatcg gggaacaggg cgcccagggc cgccacgatc atcaccccga
      841 aagcgcacat ccgggccacc ttgtacacca gcgacatcac ctcgccttgc cgcccctgcc
      901 cgatcaaact gctgaccagg gtgcttgtcg ccgatgaaaa tccccacatc gggatcatta
      961 atatcacata aaaactcctt acaatattcg aaacggccag ggccatctcc cccagcttct
     1021 cgatgaacag gaaaaaaacc agccaggcca ccatcgagat gaaatgctgc atcatcaccg
     1081 gcaaggatat ccgtatgatc ctccggtaca actgctcgtc gaaacggctg cgggcggtga
     1141 ttcgatactt tctgccatcc aggataaaaa aggtgtaaac ggtaaaatag accgtcgcaa
     1201 ccgcttccga aagggtcgag gccagcgccg ccccgccgat ccccaactcc ggaaagccca
     1261 ggttgccgaa gataaggcaa taatcgagaa aagcattcgt aaaagccagg ataaatgtgg
     1321 tgtaggtgat cacttttgtc cgtgcaatgc cgatgtaaag cccgcggtac agcatgttta
     1381 ccccggcaaa gaaaatcccc caggagcggt aaccgaggta ctcctgcaag ctgtcgtaca
     1441 cggcttctga actgataatc aggctgagca gatcgccgga gaagaacttc atcaggctga
     1501 acagggttag cgtaaggggc agcatgaaaa agaaaccgtg ccggaaggtc cgcccgatct
     1561 ccccaaacgc ctgctcgcca tgccgccggg ctatgatgat ctggctcccg atcccgaatc
     1621 cccacgccag catgctggct accaggtaaa aaatgccccc gatcgccccg gcccccaatg
     1681 ccacctgtcc gacccgcccc aggaaggccg tatcggtgat gttgatgatg ttctgcgcta
     1741 cgcttccgag tatgatcggg tatgcgatca gccaaatatc cttattggta accgacgtct
     1801 tcagtgtaac cgattttttc ggtaaattta ctccttttgc gaacaaatga ggcgggttgt
     1861 ggttaatgtg ttgggtttgt agtccgacga ctgaagccgt cggttctggc gggtttttcc
     1921 ttccgacgac tgaagccgtc ggttccatca attctggaac caacggcttt agtcgttgga
     1981 ccacgaacac aggcaaccaa cggctttagt cgttggacca cgaacacagg caaccaacgg
     2041 ctttagtcgt tggaccacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 cacaggcaac caacggcttt agtcgttgga ccacgatcac aggcaaccaa cggctttagt
     2221 cgttggacca cgatcacagg caaccaacgg ctttagtcgt tggaccacga atatgaaaat
     2281 gaaatcataa aatgaaaaca ctgatcatag gcgcaacagg gtttatcggc cgggagctgg
     2341 taaaagatct ggcggatcac ggccacacgg tggtggctgt gacaaggaac tcggggatgg
     2401 ccagggccat tctcgggaaa aaatgctcga tcgtggagtg ggacgggatt tcggtctcct
     2461 cgctggtcca ctacatggcc ggatgcgaag cggtggtcaa cctggccggg gagaatatcg
     2521 cttcgggccg gtggacggag aaaagaaaag aggccctggt cgccagccgc accaccgttg
     2581 gccgtaagct ggccgaggcc attctccgcc tcacctctcc cctgcctgtg ctggtccagg
     2641 gctcggccat cggaatctac gggaccgacg tcgattccgc atccgacgaa tcgctggctc
     2701 cgggtagcgg attcctggca aacctggtgg tggaatggga aaattcagta gcggaggccc
     2761 tgcccaatgt caaaaggctt gcctgggcgc ggacgggtct ggtgcttggc cgggatggcg
     2821 gtttgcttga aaaaatgctc ctgccgttcc gatttcacag cggcactctc ctgggatcgg
     2881 ggcggcagtg gatgtcgtgg atccacctca gcgacgaagt gcgcgcgatc cgtttcctcc
     2941 tcgaaaataa cacctcttca ggccattaca acctgacagc cccaatgccg gtgaagatgg
     3001 aagagtttat ccgcgccctg agccgaatta ccggcaaacc tgcctgggtt agggtgcccg
     3061 gagccgtgat caaagccctg atgggcgaaa tggccgccga aacggtgctg gccagtcaga
     3121 atgccctccc ggccaggctg ctccgggatg ggtttacatt tgactatccc cggctggaag
     3181 atgctttaac gaatctgctg acaaacaatg aataacacgg aaataaacct tttctggttc
     3241 aggagagatc ttcggctgga cgataatgcc ggcctgtttc acgtcctcag cagtggcctg
     3301 cccgttctcc ccattttcat ttttgatgac gacatccttc taaaacttga taataaagaa
     3361 gatgcaaggg taacattcat ttatgacgag gtcctccgta tcaaacagga aatcgaaagc
     3421 cggggaggat cattattgat caaacggggc cgcccggccg gtattttccg ggaactcacc
     3481 ggccggttcc ggatcagcaa ggtttttgcc aatcatgact atgagcctta tgcccgtaac
     3541 agggatgacc gggtcgggaa attgttgtcg gagcagggta tccggtttat tactttcaag
     3601 gaccaggtta tttttgagaa agatgagatc ctgaaagatg acggcaaatc atacaccgtt
     3661 tttaccccgt attcccgaag atggaagtcc gccctgagcg gatcatcttt cacaaagcac
     3721 aactcagcag gcttgaccgg caacttcctg aagatgatgc cgatggatgt tcctgccctg
     3781 tcatccctgg gattcaaccg gtcgcgtttg acatttccac cgcgggaacc cgatgccggt
     3841 atccttcagc attaccaccg cgaccgcgat ttcccggcgc ttcagggcac ttcccggatg
     3901 gggccgcacc tgcggtttgg caccgtgagt atccggaaac tggcggaaat ggcgcacaaa
     3961 accaatgagg tctttctgaa cgaactgatc tggagagagt tctacatgat gatcctgtgg
     4021 cactttcctc atgcagccga acgggccttc aaaccacagt acgaccggat cgcctggcgg
     4081 aacgacgagc agcagttccg ggcctggtgc gaaggccgca ccggataccc gatcgtggac
     4141 gccgggatgc gggagctgaa cgccaccggc tacatgcaca accgggtccg gatgatcacc
     4201 gccagcttcc tggccaggca cctgctgatc gactggcgct ggggcgatgc ctggtttgct
     4261 tcaaaattgc tcgacttcga gctgtcgtcg aacaacggcg gctggcagtg ggcggcaggg
     4321 accggctgcg acgccgcgcc ctatttccgg gtattcaacc cggttttgca ggctgagaaa
     4381 ttcgatcctg aaaaaaatta tatccggcag tgggtgcccg aatacggaac agggagctac
     4441 ccgcccccta tcgtggagca cagccgggca aggttgctgg ctatcgaaac gtaccaaaaa
     4501 gcgttgaaac ccgattgaac tttttatccg gacttttgtt taacaaacca aacctgaaac
     4561 ttaaacaaag attattaaca gatgaagaga aagataatat cctcattgag tgtgattttc
     4621 gccttaatcc cctttatgat ccatgcccag caaggatcgg taaaagggag gatttttgac
     4681 gaaagcaaca acgagccgat tgcatttgcc aacattattg tgttcgggac gaccatcggt
     4741 tcgacctccg acctggatgg caatttcatt ttcacggggc tggagccggg atttatcaag
     4801 ctggccgctt cggcagtcgg atatgaatcg aaggtgacca tcgatttcca ggtgaccaat
     4861 tcgaaaacat catttatgga tatcccgatg aagccgaagg agttcgagct gcaggaggtg
     4921 gttatcaagg cgtcgccgtt caagcggctg gaggagagtc ccgtttcgct gcgaacgctg
     4981 cagatctcgg agatcgaaaa gaacccgggc ggtaaccgcg acatttccag ggttatccag
     5041 gttttgccgg gcgtgtcgtc cacaccggca ttccgtaacg atgtgcttgt tcgcggcggc
     5101 ggcggcaacg aaaacgcgtt ctacctcgac gggatcgaga tcccgaacct gaaccatttc
     5161 tcgacgcagg gttccagcgg cggtccggtg ggcatcatca atgccgattt tatccgggaa
     5221 gtcgacttct acagcggcgc tttcccggcc gaccgcggca aatcgctcag ctcgctgctc
     5281 gacatgaaac tgatcgacgg caacaaggag gagtggatct tccgcggatc gctcggcgcc
     5341 agcgaccttg cattgaccac caacggcccg gtgtcggaca actccacctt tttattctcc
     5401 taccgccgtt cgtacctgca atttctgttc gacgcactgg gtctcccttt cctgcccaac
     5461 tacgacgatt tcctggcaaa atacaagatt cagttcgacc agaaaaatga gctttcggtt
     5521 atcggcctcg gctcgatcga ccgtttcgaa ctgaacaccg ggatcaagga tccggatgaa
     5581 agccaggcct acatccttgg ctatctgccg gtgcaggagc agtggtcgta taccatcggc
     5641 gccgtgtaca agcattatca caagaacggc tttaccgatg tgatcctgag ccggaacatg
     5701 ctggataacc ggtcgttcaa atacaggaac aacgatgaga acgacacact gaatttcaac
     5761 tataactccc aggaatcgga gaataagctg cgcgtggagc acaccagccg ccggggagcg
     5821 ttcaaatata cctacggcgg aggactggaa ctggcccgct acaccaacag cacctttcag
     5881 cggctttacg cgaacgatgt ttacacgccc gtcaattacg actcacagct taggatgttc
     5941 ctgtggaacg tattcgggca ggtcagcaaa aatttcttcg acgacaggct ggtcctttct
     6001 ctcggtctca gaatggatgc aaacgactat acgccttaca tggccaacct gctcaaccag
     6061 gtttccccga gattttcggc ttccttcggg ctgaccgata tctggttcct gaacttcaac
     6121 accggaaggt actaccagcg gttgccctat actactttcg gatttcgcga caacaacgga
     6181 gaactggtta acaagcggga tgatctccgc aatctctccg ccgaccacgt ggtcttcgga
     6241 ttcgaggtcc tccccgaaag ggatgccaaa ttcagcatcg aaggctttta caagtactac
     6301 aggaactacc cgttctctgt cagggattcg atcaacatag ccagcaagag cgcagggtac
     6361 ggcacattcg gcgatgagca ggtgatctcg gtttcggatg gccgggcgta cggattcgag
     6421 gtactggctc aggacaagtc gctgttcgga ttcaacgtga ttttgtcgta cacgtttgtt
     6481 gtcagtaagt ttacggataa ggacgataaa tttgtccctt ctgcgtggga caaccgtaac
     6541 atcctcaacc ttaccgttct gaagagcttt aagcgaaact gggatattgg cgccaaatgg
     6601 cgttacgttg gcggggcgcc ctacactccg gccgacctgg agttttccag ttatgttccg
     6661 gcctgggatg cccgcggccg ggaatatccc gacaattcca ggttcaacga gctgcggctc
     6721 aaagccttcc accagcttga tctccgtgtt gacaagacct atttcttcaa gaaatggtcg
     6781 ctgaatttat atattgatgt tcagaacgtt tataatttcc aggccgaaaa tccgcctaac
     6841 tataccaacc tggacaagga cggcaacaaa gtttaccagg atccccccac aaaccagttt
     6901 tatcagcttc gtgcgctgaa ctcggaatcg ggaactgttc tgcccacgct aggaattatt
     6961 gtcgaatttt aatgaccgga tcaactatga aaaccagaat attttcaatc gcattcattt
     7021 ttttcctcgg cggattgttc attgtgggta catcacggtg ctccaccgca cgaaagactg
     7081 cagattcagg ccagcctgaa atcatcaaca caaatattaa cggacagggt ctttcaatcc
     7141 gtatcaattt tacaaaaggt gaaagtcata accatcccct gatggctttc tggctggagg
     7201 acaaagacgg gaagtatata gaaactattt atatttccga atcgatcggt aagggtatct
     7261 ttcagcacgg cgaccggtcg aaaggcaggt ggcagccggg agaggtacgc aggccggctg
     7321 ctttgccata ctgggggcac aaaaggggta tccaggcacc cgacggatac tttattccga
     7381 cggtagccct gccgatgccc gacgcggtta ccgggccgac tcccaaagga gatttcatcc
     7441 tgaaaagtaa atcaacagaa caggctccgc gaatattcaa agtaatgatg gagatcaacc
     7501 agtcgtggga ctggaacgaa ttctggacaa acagcaaata ccagggcgat gtcaactaca
     7561 tgacctcaag ccagccctcg ctggtttatg aagccgtgat aaacctcgac tccccggtaa
     7621 aggaatatga gatgaaaccg gtgggtcatg gccattattc cggcgccgac gggagcctta
     7681 accccgatct cagcaccatc acgaccgccg gggagattgc ggagagcgtg aaggtaacgg
     7741 ttgaatagag actccatctc aatttctatg agactccatc tcatttttag cgaga
//
LOCUS       DAOPNH010000162         7757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10555_length_7757_cov_6.148922, whole genome shotgun sequence.
ACCESSION   DAOPNH010000162 DAOPNH010000000
VERSION     DAOPNH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_7757_cov_6.148922"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKZ74_09260"
     CDS             <1..919
                     /locus_tag="PKZ74_09260"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPM92986.1"
                     /translation="VPDRNGRMADVVLGFDNIDGYAGTHPYFGCIVGRYGNRIARAGF
                     SIDGTYHRLNANNGKNHLHGGISGFDKKIWNAAEIYREEEAGIVLTCVSNDGDEGFPG
                     ELKVKVTYTITLNNELKIEYYAETDKPTPVNLTHHGYFNLKGAGDGDILGHKLMIDAD
                     RYTVVDDELIPTGELRPVANTPLDFRNPAEIGRGIDSVPGGYDHNFVLNGDGKLKKVA
                     WLSEESTGRLMEIYTDQPGLQFYSGNFLDGTIQGKHGMIYRKHYGLCLETQHFPDSPN
                     RPEFPSTILRPGEIYRSATIYRFIINRQL"
     gene            complement(897..1754)
                     /gene="prmC"
                     /locus_tag="PKZ74_09265"
     CDS             complement(897..1754)
                     /gene="prmC"
                     /locus_tag="PKZ74_09265"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPM92987.1"
                     /translation="MTQYKELKDRSIELLKAIYPERESRAIINRVFSDILGIDPLKAV
                     VDPDMPVEPEKEILLMNCTRDLLSYKPLQYVTGKAYFLDLQLDVRPGVLIPRPETEEL
                     VSWIIRDQASAGPVSILDIGTGSGCIVLALGKRLPGAKLTALDISPAALEIARANAMK
                     HAIPVSFIEIDIMDEGAWKHLGPFDVIVSNPPYVRESEKSLMQPNVLDYEPAVALFVG
                     DDDPLVFYRKVSDLAHQHLFKTGFLYFEINEYLGDEVVRLLRNSFSEVILKQDLHGKN
                     RFIRAAGLL"
     gene            1858..2502
                     /locus_tag="PKZ74_09270"
     CDS             1858..2502
                     /locus_tag="PKZ74_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92988.1"
                     /translation="MRGDILIIGDHHRSAAENILSLTLTAIRSKKGVYIFTIAGESGA
                     GKSETAYALEGLLEKIHIRTYIIQQDDYFILPPKTNEKARIININRVGPDEVRLDLLN
                     ENIRSVIAGDMVIKKPLVIFEEDRITTETIDLSGYEVIIIDGTYTTMLEGIDCHVFID
                     RDRNDTRTDRLKRNREQQNEYLERILEIEHKIISRHKEMADIIVNKEFKAYLAR"
     gene            2499..3755
                     /locus_tag="PKZ74_09275"
     CDS             2499..3755
                     /locus_tag="PKZ74_09275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM92989.1"
                     /translation="MTGRYKRLAMLSTHGYFDPIPLLGETDTGGQVLYVLELSKALAR
                     RGIKVDIYTRWFDRNRSQIELLPGYPDVRVIRIPAGPWEFVIKEKIYSLLPELTENLA
                     AFLRENRLDYDLFHGHYVDAGIVTVDLARIFGKPAFFTAHSLGAWKRERMDGDPEQME
                     LEFNFNHRIEEEKRIYRTLPGHTVTSRLQLDKLRELYGYEEDNVEIIPPGVNIHKFKP
                     LNPGEPPKKTNLPEKYIFCLSRVDSNKGYDLLLNAFGKVCQKMDDVYLVTGGGSSNPQ
                     PREKEVIDMMLRIMKEKGIENKVIFVGHVEEHLMVPYYQNAQFFVMPSIFEPFGMTTQ
                     EAMASGIPVIASKFGGINTVLTHEKDGLLIDPKNEDEFAGAMIRLLEETAFRKQLGQK
                     ASSLIRQNYSWEAMAEKHLHFYKKYV"
     gene            3755..4609
                     /locus_tag="PKZ74_09280"
     CDS             3755..4609
                     /locus_tag="PKZ74_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM92990.1"
                     /translation="MLFIALSVIQSVLILITFKLFDRFRIDNLQAIVTNYIIAAFFGF
                     LTAGTTWSFKLLPHNDWFLTAIFLGFLFIGTFFLFALSSQKIGVAITSVTSKMSLIIP
                     VTVSILLLGESVNLFRIAGIVLAFAAFYFSLKQNEPIRWNSRYTYLPFLVLAGNGLVD
                     TTMKLADHYYISDDVTLFLSVVFSTSFLIGAAILVYRILRRKTILKLKNLAGGVILGS
                     LNFGSTYYMLKAISSFESSVVFPVTNAAIVGISGLAGFFIFREKLTWINWLGITLAIL
                     AILIISKA"
     gene            4612..5214
                     /locus_tag="PKZ74_09285"
     CDS             4612..5214
                     /locus_tag="PKZ74_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPM92991.1"
                     /translation="MNTDEYRTLAFEARGLYKEKGSKFIAVALPVSTEEEIRLARERI
                     RKEFHDARHHCFAWRIGEPPYESRYNDDGEPSGTAGKPILGQIQSFELTQILIVVVRY
                     FGGIKLGTGGLIQAYKKASMDALENGQIITKHWKVKVKISFDYLKMNEIMKIIKEEDL
                     MVTGQESGERCHISLEINKYRSLTIYNKFNSVESLEWAVF"
     gene            5530..6969
                     /gene="dnaA"
                     /locus_tag="PKZ74_09290"
     CDS             5530..6969
                     /gene="dnaA"
                     /locus_tag="PKZ74_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPM92992.1"
                     /translation="MKRTCTEVWENCLKIIKDNIHYQSYKTWFIPINPVKLENNVLTI
                     QVPSQFFYEWLEEHYVGLLKKTIKKELGSDGRLEYSILMETSYDNPDPLSIKIPATNK
                     KALANPAVSMPIDVNRGASKEIPNPFVIPGLKKIKVNSQLVENYSFENFIEGDCNRLA
                     RSAGFAVAENPGKTAFNPLYLYSNVGLGKTHLAHAIGIQVKNNYPDKTVLYVSTEQFV
                     GQYIESVRNNNQNDFIHFYQMIDVLIIDDIQFLSGKEKTQDVFFHIFNQLHQNSKQLI
                     LTSDKAPVEMKGIEPRLLSRFKWGLAADLQPPDLETRIAILQKKLYRDGIEMPDEVIE
                     YLAYSINTNIRELEGALISIMAQSSLNKKPITLDLAKQMIDKFVKSTSREISIDFIQK
                     IVCDYFNLPVDSISSKTRKREIVQARQLAMYFAKKHTKSSLATIGLHCGNKDHATVLH
                     ACRTVNNLIDTDKQFRIYVEELDKKIKMV"
     gene            7189..7668
                     /locus_tag="PKZ74_09295"
     CDS             7189..7668
                     /locus_tag="PKZ74_09295"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPM92993.1"
                     /translation="MKILFVCLGNICRSPLAEAIFTHKAKQLGLNVEANSAGFESFHR
                     GDPADPRSVAVARAHGIDLSEHRARMFAVRDFDHYDRIYVMDRNNYNDVMGVARDSKD
                     EKKVDFMLNLVHPGDNRPVPDPWYGSKDGFEKVYQMLDEACENLIHELSHPLPPLHT"
BASE COUNT         2196 a         1884 c         1786 g         1891 t
ORIGIN      
        1 ggttcccgac cggaacggga gaatggccga tgtagtcctc ggatttgaca acatcgatgg
       61 ctatgccggt acccatccct atttcggctg tatcgtcggc aggtatggta accggattgc
      121 ccgtgccggg ttcagtatcg atggaaccta ccacaggtta aatgccaaca atggtaaaaa
      181 tcatcttcac ggaggcatat cgggatttga taaaaaaatc tggaacgccg cagagattta
      241 ccgcgaagag gaagcgggga tcgtgctgac ctgtgtcagt aatgatggcg atgaaggctt
      301 tcccggggaa ttgaaggtta aggtgaccta taccattacg ttgaacaatg aattgaagat
      361 agaatattat gccgaaacgg ataaaccaac accggtcaat cttacccacc acggctattt
      421 taacctgaaa ggagcaggag atggtgatat cctgggccat aaactgatga ttgatgccga
      481 ccggtacacg gttgtcgatg atgaactcat tcccaccggt gagctgaggc cagtggcgaa
      541 cacaccactg gatttcagaa atcctgccga aatcggaagg ggcattgatt cggttcccgg
      601 aggatacgat cataactttg tcctgaacgg tgatgggaag ttgaaaaaag ttgcctggct
      661 aagtgaagaa tcgaccggaa gacttatgga aatctacacc gaccagcccg gactgcagtt
      721 ttacagcggc aatttcctgg acggaactat ccagggaaaa catgggatga tttaccggaa
      781 acactatgga ttatgtcttg agacacagca ttttcccgac tcgcccaacc ggccggagtt
      841 cccttctact atactccgcc ccggcgaaat atacagatcg gcaactattt acaggttcat
      901 aataaaccgg cagctctgat aaaccggttc ttcccatgca aatcctgttt aaggatgact
      961 tcactgaaac tgttacggag taaccggacg acttcatcac ccaaatactc attgatctca
     1021 aaatacaaga atccggtctt aaacaagtgt tgatgagcca gatcggagac tttccggtaa
     1081 aacaccagcg ggtcatcgtc gccgacaaat aatgccacag ccggctcgta gtcaagcaca
     1141 ttcggctgca tgagagattt ctcggattca cggacatacg ggggattgct gacaatgaca
     1201 tcaaatggtc ccaaatgctt ccaggcccct tcatccatga tatcaatctc tataaatgat
     1261 accgggatag cgtgcttcat agcattggcg cgtgcaatct ccagggctgc aggagaaata
     1321 tccagtgcgg tcagctttgc accgggcaat cttttcccca acgccaggac aatgcatccg
     1381 cttcccgttc cgatatctaa aatggagaca ggcccggctg aagcctggtc gcggataatc
     1441 cagctgacaa gctcttctgt ttcggggcgt gggatcaaaa cacccggtcg cacatccaat
     1501 tgcagatcaa gaaaatatgc ctttccggtc acatattgca aaggtttata actgagtaaa
     1561 tccctggtac agttcattaa caggatttct ttttcaggct caaccggcat atccggatca
     1621 accacggctt ttagcggatc tatccccagg atgtcgctga acactctgtt gatgatggcc
     1681 cggctctccc gttcgggata tatcgccttc agcaattcta tcgatctgtc tttaagctct
     1741 ttatactgag tcatacaaat aatccggccg gtttaaaatt tctgaagaat ttcgtcccgg
     1801 aaggtaagaa tattctaatt ttgactttac gttaaagatt aatttatctg aattaatatg
     1861 aggggagata tcctgatcat tggtgatcat caccggtcgg ctgcggaaaa tattttaagc
     1921 ctgacgctga cggcaatccg cagcaagaag ggagtttaca ttttcaccat tgccggggaa
     1981 tcgggcgccg ggaaatcgga aacggcctat gcgctcgaag ggctgctcga aaaaatccac
     2041 atcaggactt acatcattca acaggatgat tactttatcc ttcccccgaa aaccaatgaa
     2101 aaagctagaa ttatcaatat caacagggtt ggccctgacg aagtcaggct cgatctgctg
     2161 aatgaaaata tccgctcggt tattgcaggg gatatggtta taaagaaacc ccttgtcatc
     2221 tttgaagaag accggatcac aaccgaaacc atcgatttat caggctatga agtgatcatc
     2281 attgatggca cctacaccac aatgctggaa ggaattgatt gtcatgtatt tattgacaga
     2341 gaccggaacg atacccgaac tgaccggttg aaaaggaacc gggaacagca gaatgaatat
     2401 ttggagcgaa tcctggaaat cgaacacaaa ataatttcgc ggcacaaaga aatggccgat
     2461 attatcgtaa ataaagaatt taaagcctat ttagccagat gacgggccga tataaacgcc
     2521 tggccatgct cagcacgcat ggctattttg atccgatccc gctgttagga gaaaccgata
     2581 cgggcggaca ggtcctgtat gtgctggaat tatccaaagc gcttgcccgc aggggaatca
     2641 aggtcgatat ttatacccgc tggttcgacc ggaacaggag ccagatcgag ctcctgcccg
     2701 gttacccgga tgtgagggtg atccggatac cggcaggacc atgggaattc gtgatcaagg
     2761 agaagatcta ttcgctcctg cccgaactga ccgaaaacct ggccgcgttc ctccgggaaa
     2821 accggctgga ttacgacctg ttccacggcc attatgtgga tgcagggatt gttacggtcg
     2881 acctggcccg catcttcggc aaaccggcat tcttcaccgc ccactccctg ggggcctgga
     2941 aacgggaacg gatggacggc gatccggagc agatggaact cgagttcaat ttcaatcacc
     3001 gaatcgaaga ggaaaaaagg atctaccgga cattgccggg tcataccgtt acttcccggt
     3061 tgcagctcga taaactcagg gaactctatg gttatgagga agataacgtt gagatcatcc
     3121 cgcccggggt gaacatccat aaattcaagc cgttaaatcc cggcgagccc ccaaagaaaa
     3181 ccaacctgcc cgaaaaatac attttttgcc tgagccgtgt cgacagcaat aaagggtatg
     3241 acctcctgct aaatgcgttc ggaaaggtgt gccaaaagat ggatgatgtt taccttgtga
     3301 ccggtggcgg ctcatcaaac ccacagccca gggaaaaaga ggtgatcgat atgatgttgc
     3361 gcatcatgaa ggagaaaggc attgaaaaca aagtcatctt cgttggccat gtggaagagc
     3421 acctgatggt accatattat cagaatgcac agttctttgt catgccctcc attttcgaac
     3481 cattcggaat gaccacgcag gaggccatgg ccagcggaat tcccgtcatt gcctccaaat
     3541 tcggcggtat caatacggta ttaacgcacg aaaaagacgg cttactcatc gatccgaaaa
     3601 acgaagatga atttgccggc gccatgatcc ggctactgga agaaaccgct ttccggaagc
     3661 aactcgggca aaaagcctcc tcactgatcc ggcaaaatta cagctgggaa gccatggcag
     3721 aaaagcacct tcatttctac aaaaaatatg tttaatgctc tttatagccc tgtcggtcat
     3781 acaatcggta ctgatcctga tcacgttcaa actgttcgac cggttcagga ttgacaacct
     3841 gcaggctatt gtcaccaatt acatcattgc ggcgtttttc ggatttctca ctgccggtac
     3901 aacctggtca ttcaaacttt taccgcataa cgactggttc ctgaccgcca ttttcctggg
     3961 attccttttc atcggcacct tctttctgtt tgccctctcc tcacagaaaa tcggtgtcgc
     4021 cattacttcc gtgaccagca agatgtcgct tatcataccc gtaacggtca gcatcctgtt
     4081 actgggcgaa agcgtcaatc ttttccggat cgccggcatc gtactggctt ttgccgcttt
     4141 ttatttttct ctgaaacaaa atgaacccat ccggtggaat tcaaggtaca cgtacctgcc
     4201 ttttcttgtt ttagcaggta acggcctggt agacacgacc atgaaactgg ccgatcatta
     4261 ctatatcagc gatgatgtta ctttgttttt atccgtggta ttctcgacaa gctttcttat
     4321 aggcgcagca atactggttt acaggatatt aaggcgaaaa acaatattga agttaaagaa
     4381 cctggccggg ggagtgatcc tgggatcgct gaatttcggc tctacctatt atatgttaaa
     4441 agccatctcc tcttttgaaa gttcggtcgt gtttcccgtg actaatgctg ctatcgttgg
     4501 catatcaggc cttgccggct tttttatttt tcgtgagaaa ctgacatgga tcaactggct
     4561 gggcattacc ctggccatcc tggccatatt aattatatca aaggcctgag tatgaatacg
     4621 gatgaataca ggacacttgc gtttgaagcc cgcggcctct acaaggagaa gggcagcaaa
     4681 tttatagccg tagctttgcc ggtatccacg gaggaagaga tcaggctggc cagggaaaga
     4741 atccggaaag aattccatga tgcccgtcat cactgctttg catggagaat tggtgaacca
     4801 ccttatgaat cgcgatataa tgacgacgga gagccgtcgg gtaccgcagg aaaacccatt
     4861 ctcggacaaa tccagtcctt tgaacttacc cagatcctga tcgtagtcgt acgatacttc
     4921 ggcggtatta aactgggaac gggaggattg atccaggctt ataaaaaagc cagtatggat
     4981 gcccttgaaa acggacagat cataacaaag cactggaaag taaaagtgaa gatcagcttc
     5041 gactatttaa aaatgaatga aataatgaag ataatcaaag aagaggatct catggtgacc
     5101 ggccaggagt cgggtgaaag gtgccatatc tctttggaaa tcaacaaata tcgttctctg
     5161 accatataca ataaattcaa ttcggttgaa tcactggaat gggccgtttt ttaacaaaat
     5221 atttaacaga ttttctttga atctaatgat tttattttgt actttttcaa actcaaattg
     5281 attttatcca aaactgagag aatcaataga ttaactttag gtaagaagag ggggtcaaat
     5341 ttgcctatat aagctaaaaa ttctttaatg tcaagcatga aattactttt ttttttaact
     5401 tttttaatga cacttttgtt gaataacttc acagggcacc agccaggaat gagtgacctg
     5461 attttcaagc ggaaataata aacagagaaa agaaaatcat tatttaacaa gcggaacgag
     5521 ggttttagca tgaaaagaac ttgtacggag gtatgggaaa attgcctgaa gattataaag
     5581 gataatatcc attaccagag ctacaaaaca tggttcattc ccattaatcc tgtaaaactg
     5641 gaaaataatg tgctaactat ccaggttccc agccagttct tctatgagtg gcttgaagag
     5701 cactatgtcg ggctgctgaa aaagacgatc aaaaaggagc tgggttcaga cgggaggctg
     5761 gaatacagca tcctgatgga gaccagttat gacaatcccg accctctttc gatcaaaatc
     5821 ccggcaacca ataaaaaagc gctggccaac ccggcggttt cgatgccgat cgatgtaaac
     5881 cggggcgcct caaaagagat ccccaacccg ttcgtcatcc ccggcctgaa aaaaattaag
     5941 gtcaattcac aactggttga aaattactca ttcgagaatt tcatcgaagg agactgtaac
     6001 cgcctggccc gttctgccgg attcgccgtt gctgaaaacc cgggcaaaac ggctttcaat
     6061 ccgctttacc tttattcaaa cgtcggcctg ggtaaaaccc acctggccca tgccatcggt
     6121 atccaggtca agaataatta ccccgataag acggtcctct atgtttcaac cgagcaattt
     6181 gtcggacaat acatcgaatc ggtcagaaat aataaccaga acgacttcat ccatttctac
     6241 cagatgatcg atgtgcttat catcgacgat atccagttcc tttcgggaaa ggaaaaaacg
     6301 caggatgtgt tctttcatat cttcaaccag cttcatcaga acagtaagca actgatcctg
     6361 acctccgaca aagcgccggt ggagatgaaa ggcatcgagc cacgcctcct ctcccgcttt
     6421 aagtggggtt tggccgccga tctgcaaccg cctgaccttg aaacccgtat cgcgatcctt
     6481 cagaagaaac tctaccgcga cggcatcgaa atgcccgacg aagtgatcga atacctggcc
     6541 tacagcatca acaccaacat ccgcgaactg gaaggagcgc tgatctccat catggcccag
     6601 tcgtcgttga ataaaaagcc gatcaccctt gacctggcca agcagatgat cgacaaattc
     6661 gtcaagagca cttcgcgcga aatttccatc gacttcatcc agaagatcgt atgcgactat
     6721 tttaacctcc cggtcgattc catcagctcc aagacccgga aacgcgagat cgtccaggca
     6781 cgccagttgg ccatgtattt tgccaaaaag cacaccaaat cgagcctggc caccatcggc
     6841 ctgcactgcg gcaacaagga ccatgcgacg gttctccacg catgccgtac cgtcaacaac
     6901 ctcatcgaca ccgataagca gttccgcatt tacgtggagg aactggataa gaagatcaag
     6961 atggtgtagt tcagttggac agcctgtccc gagcgaagcg agggattgga cagttggaca
     7021 gtcgcattct tccaaaattt aatttgcact accctattcc ccgcagggga tataaataaa
     7081 taaaacaggt ttccggaagg caattaaatt tcgcacagcg gctctatgtg taactatgtg
     7141 tcctatatgc ctatgtggtt taaagccaaa tttctttatt tttgatccat gaagatactt
     7201 tttgtatgcc ttggtaacat ttgccgttct cctctggctg aggcaatttt cactcataag
     7261 gcgaagcaac tgggacttaa tgttgaagct aactccgcgg gatttgaatc gtttcaccgg
     7321 ggcgatccgg ccgatccgcg ctccgtagcc gtcgcccgcg cacatggcat cgacctgtcg
     7381 gaacaccggg cccggatgtt cgccgtccgc gatttcgatc attatgaccg catctatgtc
     7441 atggaccgta ataattataa tgatgtgatg ggtgtggccc gtgacagcaa ggacgaaaaa
     7501 aaagtcgact ttatgctgaa cctggtccat cccggagaca accgcccggt ccccgatccc
     7561 tggtacggaa gcaaggatgg ctttgaaaaa gtctatcaaa tgctcgacga agcctgcgag
     7621 aatttaattc atgaactctc acacccctta ccacccttac acacctgaac catgattccc
     7681 gaccttcaaa ccatacaagc ggcagccata aggatcaaac cctacatcca tcgcacaccg
     7741 gtgctcacat cccggag
//
LOCUS       DAOPNH010000163         7629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10833_length_7629_cov_5.937285, whole genome shotgun sequence.
ACCESSION   DAOPNH010000163 DAOPNH010000000
VERSION     DAOPNH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_7629_cov_5.937285"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..649)
                     /locus_tag="PKZ74_09300"
     CDS             complement(32..649)
                     /locus_tag="PKZ74_09300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPM92994.1"
                     /translation="MTRIQPINTNRYIVHLDLDTFFVSVERLMNARLVGRPVIIGGTS
                     DRGVVASCSYEARQYGVHSAMPMRTARMLCGDAIIVRGDMDLYTRYSRVVTDIIAERA
                     PVYEKASIDEHYLDITGMDRFFGCLKWTRELRQTIMKESGLPISVGLSVNKTVSKIAT
                     GEAKPNGELEVPPERVHSFLNPLSIKKIPMIGDKVYHLLRYLFDG"
     gene            861..1634
                     /locus_tag="PKZ74_09305"
     CDS             861..1634
                     /locus_tag="PKZ74_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM92995.1"
                     /translation="MRKRLGRTQDDIAFSLNMKRPTLSGYENGIAEPGMEALIAFSRY
                     FKISIDTLVKEDLSKLAESELSQLERGYDVFITGGKLRVLATTVNTDNEENVEMVAET
                     AKAGYRSGFADPEFIRVLPVFQLPFLSRDRKYRAFQINGDSMLPIPDKSWVIAEFVQN
                     WFTIRNGYPYIILTMEDGIVFKVAENKLKAEQKLTLVSLNPLYEPYDVHVKDIREVWK
                     FVHYVSSQIPEPNQMKENLASTVMTMQKELKEIQMKLKL"
     gene            1711..3057
                     /locus_tag="PKZ74_09310"
     CDS             1711..3057
                     /locus_tag="PKZ74_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPM92996.1"
                     /translation="MYAIIDIETTGLNARKDRITEIAIFIHNGEKIVDQFETLINPEC
                     RIPYHITAMTGISNKMVAEAPHFYEVAKRIVEMTDGNTFVAHNAMFDYNFIRCEFKRL
                     LYDYSRKTLCTKKLSRKLMPGLKSYGLGSLAKFLDIKNKARHRAAGDALATVELLEHL
                     MRIERNIENIPLKGLNSNLSPEIIKDLPEVPGVYYFHDESGNILYIGKSVNIKSRVMS
                     HFSNNDHKRELELREKTASITYEPTGSDLVASLLESAEIKKYKPVFNRSLRRSLFQYG
                     LYSHTGSDGYIHLAIEPIKGDKLPLTSYGSRKEGVSHLQRLLDEFNLCQKLCGLYSSK
                     GSCFDHHMGICRGACVGAEPPSSYNDRVIDAISRYAYRNKNFFIVDDGRFAHECSVVK
                     VENGRFIGFGYVELNEIGNQSRILHDIIKPFEDNRDAHVIIRGYLRKNQFEKMIVY"
     gene            complement(3062..3823)
                     /locus_tag="PKZ74_09315"
     CDS             complement(3062..3823)
                     /locus_tag="PKZ74_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPM92997.1"
                     /translation="MNSSTVQYIALIWILCAVIIFPVLLKITAPYGRHSKKTWGPVIN
                     NKLGWILMEAPSFIIFVSLVLFGPNTPDKIVWVLLAMWGIHYFNRSFVFPLRTKTQGK
                     KMPVIIMFSAIFFNLINASLNGYWLGFVAPPYPDSWMTDPRFITGGIIFITGFVINQM
                     ADNKLIGLRKGGQKGYFIPRGGLFNYISCPNFFGEIVEWAGFAIMAWNLPALSFAVWT
                     AANLIPRAIDHHKWYKGHFKTEYPAKRKAVIPFLV"
     gene            complement(3861..4838)
                     /locus_tag="PKZ74_09320"
     CDS             complement(3861..4838)
                     /locus_tag="PKZ74_09320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM92998.1"
                     /translation="MKIAVTGANGHVGANLCRRLISEGHQVKALVHTRSEALKGLDVG
                     LIQGSVSDLDSLRKLCKDTEIVFHLAALISIDGQKDKLLRVNVEGTHNLVKVIKENGV
                     KRLIHFSSIHSLDHRPFNEPMDETRPLINGGPMWYEITKSRAEQFIIDESAKGLDAVI
                     INPTAIVGPNDFKPSLVGTVLIRLYNNSLPALVPGGYNWVDVRDVVSGAVAAMAKGQT
                     GERYILGGRWVSVRDLALIVEKVTGRKTVRMMLPTVIAKIGVPFIKVYAGLAKQEPLY
                     TFESLRALREVNTMISCAKAKVELGYHSRDFEETVRDTLDWFKAHDYIR"
     gene            complement(4852..5454)
                     /locus_tag="PKZ74_09325"
     CDS             complement(4852..5454)
                     /locus_tag="PKZ74_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM92999.1"
                     /translation="MKVCLIIRGLIRSASDRIYESLNQFFQGKTEIPGTNNDIKSNDA
                     NANLIYMEWDLHQGIEDIMINDISFFISKLLSHKFINTLSIIRKRILISLEFDVLDNG
                     ENQVNETFLPSGEPEPKIVFFNKSAGRTTMALPELFTRFLFRATHLSHKIKHLKTILC
                     KFFYSLIITNRIDYDRINRRLSLHTCAVNLSFIPFFHKRN"
     gene            6154..6831
                     /locus_tag="PKZ74_09330"
     CDS             6154..6831
                     /locus_tag="PKZ74_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93000.1"
                     /translation="MQINSGNKEVLSTLNTVVNSVVTENIGLREYLKQLQATHTDNII
                     GMANQHLASLSRSTGRNYILNEESIQKLKEYKGNLRHIGSILKTAVIFTENLSPANLD
                     DPVIIRPFSISFDSYQVDDKPEYAARKIDSRMSKAMLLLDKLENAARVLRSNNLPLIG
                     TRMGQALDPPVTAPAITDALGKNANLIRQLLMKYPEKWEIIRKDFKPLLNQLRKIEPG
                     LSETGTD"
     gene            6865..>7629
                     /locus_tag="PKZ74_09335"
     CDS             6865..>7629
                     /locus_tag="PKZ74_09335"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPM93001.1"
                     /translation="MKKLNYFIFLIICPLVIHSQEYTLTHDGITRTYRLHLPVNFSPD
                     SLYPLVINMHGLGSNAWEQEIYSAFNTVSDTGRFIVACPNGLNSTWNVSSSGGTDDVG
                     FISALIDTLSVLYSVDLQRVYATGMSMGGFMSYRLACELSDRIAAIASVTGLLAFSPC
                     MPSRPVPVAQFHGTDDPVVPYAGVASTINYWISTNQCPDSAIVTDLPNIDTTDNSTVI
                     LYDYSPCTDSASVLLYSIMNGEHTWPGADILIGVTNQ"
BASE COUNT         2186 a         1797 c         1634 g         2012 t
ORIGIN      
        1 gccggaacgg gtccacagtt ttctcaaccc gttatccatc aaaaagatac cgcaacaggt
       61 ggtaaacctt gtcgccgatc atcggtatct ttttgatgga taacgggttg agaaaactgt
      121 ggacccgttc cggcggcacc tccagctcgc cattaggctt ggcctcgccc gtggcaattt
      181 tcgaaacggt cttgttcacc gataacccta ccgagatagg gagcccactc tctttcatga
      241 tcgtctgcct cagctcccgg gtccatttca ggcagccgaa aaaacggtcc atccccgtga
      301 tgtcaaggta gtgctcgtca atcgaagcct tctcgtacac gggggcccgc tcggcaatga
      361 tatccgtcac caccctggaa taacgggtgt aaagatccat gtcgccccgc acaatgatgg
      421 cgtcgccaca cagcatccgg gccgtacgca tcggcatggc cgagtgtacc ccgtactgcc
      481 gcgcctcata actgcaactg gccaccacgc cgcggtccga cgtgccgccg atgatcacgg
      541 gccggccaac cagccgggcg ttcatcagcc gctctaccga cacgaaaaaa gtgtcgagat
      601 cgagatggac gatatatcgg tttgtgttaa tcggttgtat ccgtgtcatc cgtgtgctaa
      661 ttttttttta ttttcttctt gacatcgatc attttttatt ataattttga ttaaaaatta
      721 atcaaatttg tgattacaat ttaatcaaat tatcaataca agtcaagaga aaaaaaatat
      781 ttttaaccga ccgggtattt tttaccaaaa aaccggatta atagatataa aacatgtatt
      841 tcagttcaaa tatcaaattg ttgcggaagc gcctgggccg tacccaggac gatatcgcgt
      901 tctcgctgaa tatgaaaaga ccgacattaa gcgggtatga aaacggcatc gcggagccgg
      961 ggatggaggc attgatcgcc ttcagccggt attttaaaat ctccatcgat accctggtca
     1021 aagaagacct gtcgaaactg gccgaaagcg aattgtcgca actggaacgc ggctacgatg
     1081 tcttcatcac cgggggtaaa ctccgtgtgc tggcaactac cgtgaacacc gacaacgaag
     1141 aaaacgttga aatggtggcc gagacagcca aagccggcta ccgcagcggc ttcgccgacc
     1201 cggaattcat ccgggtactg cccgtgttcc agctcccgtt cctgtcacgc gaccggaaat
     1261 accgcgcttt ccagatcaac ggcgactcga tgctgcccat ccccgataag tcctgggtaa
     1321 tcgccgagtt cgtccagaac tggtttacca tccggaacgg ctatccgtat atcattctta
     1381 caatggaaga cggaatagtg ttcaaagtgg cggaaaataa actgaaagca gaacagaaac
     1441 tcacgctcgt ctcgctcaac ccgctctacg aaccgtatga tgtccatgtg aaagatatcc
     1501 gcgaagtctg gaaattcgtt cactatgttt catcccagat acccgaacct aaccagatga
     1561 aagaaaacct ggcctcaacc gtcatgacta tgcaaaaaga actgaaagag attcaaatga
     1621 aattgaagct ttgataaata acccggaatt tggtccgcca gccggcggat ggaatttgga
     1681 atttggaatt tggaatttga accctaaggt atgtacgcaa taatcgacat cgaaaccaca
     1741 ggcctgaacg cccgcaagga ccgtatcacc gagatcgcca tctttatcca caacggggaa
     1801 aaaatagtcg atcagtttga aacactgatc aatcccgaat gccggatacc ttatcatatt
     1861 acggccatga ccgggatcag taataaaatg gtcgcagaag cgcctcattt ctatgaggtt
     1921 gcaaagagaa tcgtcgaaat gaccgacggg aacacctttg tagcccataa tgccatgttc
     1981 gactataact tcatacgatg tgaattcaag cggctgcttt acgattattc ccgaaaaacg
     2041 ctgtgcacta aaaaactcag caggaaactg atgccaggcc tgaagtcgta cggcctgggg
     2101 agccttgcca aatttctgga tatcaagaat aaagcacgcc accgggccgc cggcgatgcg
     2161 ctggcaacgg tcgaactgct cgaacacctg atgcggatcg aaagaaatat tgagaatatc
     2221 ccgctgaaag gactgaactc aaacctctcc ccggagatca tcaaagatct gcccgaagta
     2281 cccggcgtct actattttca cgatgagtcg ggcaatatac tctacatcgg aaaaagcgtc
     2341 aatattaaaa gccgggtgat gtcgcatttc agtaataatg accataaaag agaactcgag
     2401 ctgagagaga aaacagcttc gatcacttat gaaccgacag gcagcgacct cgtggcttcg
     2461 ctgctggaat cggctgagat taaaaaatat aaaccggtgt tcaacaggtc gctgagaaga
     2521 tctttgtttc agtatggact ttattctcat accggttccg atggctatat acatttggcc
     2581 attgaaccca tcaagggcga caaactgccg ctgacttcct acggaagccg caaagaaggc
     2641 gtcagtcacc tgcaacggtt actcgatgaa ttcaaccttt gtcagaaact ttgtggtctg
     2701 tattcgtcaa aagggagctg cttcgaccac catatgggca tctgccgcgg cgcctgtgta
     2761 ggcgccgaac ccccgtcaag ctataacgac cgggtgattg atgccatcag ccggtatgcc
     2821 taccggaaca agaatttctt tattgtcgac gatggccgct ttgcccacga atgcagcgtc
     2881 gttaaagttg aaaacggacg atttatcggt tttggctatg tggaactcaa tgaaatcggc
     2941 aaccaatccc gcatccttca cgacatcatc aaaccgttcg aggataatcg cgatgctcac
     3001 gtcattatcc gggggtacct gcgtaaaaac cagtttgaaa agatgattgt gtattaaaac
     3061 gtcaaactaa aaacgggatc accgccttcc gcttggccgg gtattccgtc ttgaaatgtc
     3121 ctttgtacca tttgtggtga tcaatagcac gggggatcag gttggctgcc gtccatacgg
     3181 cgaaggaaag tgccggcagg ttccatgcca taatggcaaa cccggcccac tcaacaatct
     3241 ctccgaaaaa gttagggcaa ctgatatagt tgaataaccc tccgcgggga atgaaatacc
     3301 ctttttgtcc gccttttctt aacccgatta acttgttgtc tgccatctgg ttgataacaa
     3361 aaccggtgat gaagatgatc ccgccggtaa taaaccgggg atcagtcatc caactatcgg
     3421 ggtagggcgg agcgacgaat ccgagccagt atccgttcag cgaagcattg ataaggttga
     3481 agaaaattgc cgaaaacatt atgatcaccg gcatcttctt tccttgcgtt tttgtcctta
     3541 atggaaaaac aaaagaccgg ttaaaataat gtatgcccca cattgccaat aaaacccaca
     3601 cgattttatc cggtgtattc ggtccgaata ataccaggga aacgaaaatg ataaaggagg
     3661 gtgcctccat aagaatccat cccagcttgt tgttgatcac cggcccccag gtttttttac
     3721 tgtgccttcc atagggtgcc gtaatcttta acagaaccgg aaagatgata acagcgcaaa
     3781 gaatccagat taatgcaata tattgaacag tagatgaatt cataatttag ttaaattaaa
     3841 agtttaaatg attgaattaa ttaccggatg taatcatgag ccttgaacca atcaagtgta
     3901 tccctgacag tctcttcaaa atctcttgaa tgatatccaa gctcaacctt tgccttagcg
     3961 caggaaatca tggtattgac ctccctcaaa gccctgagcg attcaaaagt atacaatggt
     4021 tcctgctttg ccagcccggc atatactttt atgaaaggaa ccccaatctt tgcaataacc
     4081 gtaggaagca tcatcctgac tgtctttcgc ccggtcactt tttcaacgat aagagccaga
     4141 tccctgacac ttacccatct gccacccaga atgtaacgtt cgccggtttg cccttttgcc
     4201 atggcagcaa cggcacctga tactacatcc ctgacatcca cccagttata ccctcccggc
     4261 accagtgcgg gcaaggaatt attgtacaaa cggatcagca ctgtacctac cagagatggc
     4321 ttgaaatcat tcgggccaac gatcgccgtc gggttgataa tcaccgcatc cagacctttt
     4381 gccgattcat cgataataaa ttgttctgcc ctggatttgg ttatctcgta ccacatgggc
     4441 ccaccgttga tcaacgggcg tgtctcatcc atgggttcat tgaagggcct gtgatcaaga
     4501 gaatggatgg aactgaaatg aataagccgc ttcacaccat tctccttgat aacctttacg
     4561 aggttatgcg taccttctac atttaccctc agcaacttat ccttttgccc atcgatcgaa
     4621 atcagcgctg cgaggtgaaa aacaatttct gtgtctttgc acagtttgcg cagggaatcc
     4681 aggtcgctga ccgacccttg aataagcccg acatccagcc ctttaagcgc ttcagaacgg
     4741 gtgtgtacca gggctttaac ctggtgtcct tcagagataa gtctacggca aaggtttgcg
     4801 ccaacatgcc cgttggctcc tgtaactgca attttcatgc agcgaattta gtcaatttct
     4861 tttgtgaaag aagggaatga aagataaatt gactgcacat gtatgcaggc tcagccgccg
     4921 gttaatgcga tcataatcaa tcctattcgt gataattaat gagtagaaaa atttacaaag
     4981 tatggttttg aggtgcttga ttttgtgtga caaatgggta gccctgaaca ggaaccgggt
     5041 aaataattca ggtaatgcca ttgtcgtccg acctgctgat ttattgaaaa agacaatttt
     5101 tggctctggt tcgccggaag gaagaaaggt ttcatttacc tggttctccc cattatccaa
     5161 aacatcaaat tcaaggctta taaggattct ttttctaata atacttaaag tgttaatgaa
     5221 tttatgtgat aataacttac taataaagaa tgatatatca ttaatcatga tgtcttcaat
     5281 tccctggtga agatcccact ccatgtagat taagtttgca tttgcatcgt tggattttat
     5341 gtcattattg gttcccggga tttcagtttt cccctgaaag aattggttta aggattcata
     5401 aatacgatcg gaagcagacc taattaaacc acgtataatt aaacagactt tcatttttaa
     5461 tttttttact taaatgaata tccagtatta aagcaaaaat aatattggta attggttaat
     5521 atcaagtaat ttaagtgaaa tcatcgtact taatatttta taaagaatat ttaaggaaag
     5581 aattaaacag attaagaata attttaaata tacttaagaa tacttaattt ttgtattaac
     5641 tttgttgaaa ctcaaccaac catgccggct ttgttttcgt tcataggaaa tgaatttgaa
     5701 agccttttcc agcattccga cctgtttttg cctggaatcg agaagttggc tggaatttat
     5761 tatcatcctg aatcccgtga attgaaaata atttccaggt ggcggaacgg agctgacccc
     5821 tattccaatg attttgttca gaaaagggaa aatttggtaa tccttcaaaa atatcgttca
     5881 gacgaaaagc cttattcctg gatcaataaa tctgatcttc cgtttgaaat tcctgacaaa
     5941 gagcatcggt tatcacccga ccttttttcc gaactggata atgtaatcct tttacttagg
     6001 atgcctaacg cctttgatca tcttagcgat ttgctcctga tttactttaa ccgtaatctc
     6061 ggaacttttg gcctgagcaa gtccgataag gtattaacca ctgaaaataa aacaattata
     6121 ggctatctgt tataccatca gtttaaatca attatgcaaa ttaattccgg gaataaggaa
     6181 gtcctgagta ccctgaacac cgtagtcaat tctgtggtaa cagagaatat cggcctcagg
     6241 gaatatttga aacaacttca agccacccat accgacaata taatcggtat ggccaatcag
     6301 catcttgcct ccctttcccg ctcgaccgga agaaactaca tactgaacga agaatccatc
     6361 caaaaactga aggaatataa aggtaatctg aggcatatcg gatccatcct taaaacagcc
     6421 gtgatcttta ccgagaatct atccccggca aatttggacg atcctgtcat aatacgcccg
     6481 ttcagcatca gctttgatag ctaccaggta gatgacaaac cggaatatgc cgcaaggaaa
     6541 attgacagca gaatgtcgaa agctatgctt ctgctggata agctggaaaa tgcagcaaga
     6601 gtgctgagaa gcaataatct tcccctgatc gggacgagaa tgggacaggc tttggatccg
     6661 ccggtaaccg ctccggccat tacggatgcc cttggcaaga atgcaaacct tatccgccag
     6721 ctcctgatga aatatcctga aaaatgggaa atcatccgga aagatttcaa accgctgcta
     6781 aaccagctca ggaaaattga acctggcctc tctgaaaccg gaaccgattg aaagtattac
     6841 ccttatcttt gttaaaattg tcatatgaaa aagttaaact atttcatttt tcttataata
     6901 tgtccattag taattcattc acaggagtat acattaactc atgatggaat tacacgaact
     6961 tatcgacttc atctaccggt aaacttttca ccggatagcc tttatccatt ggttattaat
     7021 atgcacggcc tgggttctaa cgcatgggag caggagatct actcggcctt taatactgtt
     7081 tcggataccg gccggtttat tgtcgcctgt cccaatggcc tgaactctac ctggaacgtt
     7141 tcgtcatcgg gtggaaccga tgatgttgga tttatttctg cattgataga cacacttagc
     7201 gttctgtata gtgttgattt gcaaagagtt tatgcaaccg gaatgtcgat gggaggtttc
     7261 atgagttacc gcctggcgtg tgaactctcc gacaggatcg ccgctattgc ttcggttacc
     7321 ggtttgctgg ctttttcgcc ttgtatgcct tcacggcccg ttcctgtggc ccaatttcat
     7381 ggtactgacg atccggtggt gccatatgcc ggagtggcat caaccatcaa ttattggatt
     7441 tcaaccaatc aatgccctga ttcagctata gttacagacc tgcccaatat tgacaccacc
     7501 gataattcaa cggtaatcct ttacgattat tcaccttgca ccgattctgc cagcgtactg
     7561 ctttacagca taatgaacgg agaacacacc tggccgggag cagatatact aataggtgta
     7621 actaaccag
//
LOCUS       DAOPNH010000164         7596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10897_length_7596_cov_7.275958, whole genome shotgun sequence.
ACCESSION   DAOPNH010000164 DAOPNH010000000
VERSION     DAOPNH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10897_length_7596_cov_7.275958"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..1876
                     /gene="ltrA"
                     /locus_tag="PKZ74_09340"
     CDS             431..1876
                     /gene="ltrA"
                     /locus_tag="PKZ74_09340"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HPM93002.1"
                     /translation="MSKVKKAANADNNRHYPPEAGVELRGKEGERSFAPASAEGEDGN
                     KVNASQMLEKVLDRENLNLAYKRVKKNGGSHGVDGMKVDELLPYLKRQGESLKRSLLE
                     GKYRPQPVRRVEIPKPEGGIRLLGIPTVVDRMIQQAIAQVLSPIFEEEFSKYSYGFRP
                     GRGAHQAIEQARQYINEGYKVVVDLDLEKFFDRVNHDKLMYLLAKRITDKRILKLIRA
                     YLESGVMLGGLISPSEEGTPQGGPISPLLSNVMLHELDKELERRGHRFCRYADDSNIY
                     VKSKKAGNRVMESVSQFIEKRLKLKVNREKSAVDYPTKRKFLGYSFYYNRGVASIRVH
                     AKPLSKLKAKLKKLTGRSIGISMEARTDKLNQTIRGWVNYYQLADMGKHCQKTDEWLR
                     RRLRMCYWKQWKNIGARHYNLVRLGIPDSKAWEFANTRNGHWHTANSPILASTLTNEF
                     FRKLGLTGLSDVYLISLSLRTAVCRTARTVV"
     gene            1944..4049
                     /gene="recG"
                     /locus_tag="PKZ74_09345"
     CDS             1944..4049
                     /gene="recG"
                     /locus_tag="PKZ74_09345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPM93003.1"
                     /translation="MTRDFIQTPVEFLKGVGPKRAELLRSELNIHHFGDLLNYYPFRY
                     IDRSRIFKISEIEPDTAYVQIRGTVTNIRIIGEKRARRMSALLRDDTGEIELVWFQGI
                     KWIEGTILPNVEYIVFGKPTIFNRRYNIAHPEIERAEDFLRTVPQTLHGIYSTTEKLK
                     NRGLNNKNLVKLIRELIIQAEGKLHETLPDEIIRKLSLLGREEALRNIHFPLNQQLLQ
                     KAQARLKFEELFFIQLGLLKDKMIRQQRISGFVFSRVADKVNRFYSEKIPFNLTEAQK
                     KVIREIRKDLGAGKQMNRLLQGDVGSGKTLVALMAMLIAIDNGFQSCLMAPTEILASQ
                     HLATLTRMTDGLGIGIGLLTGSTPAAKRREMHEKLQSGAMDILIGTHALLEDPVVFKN
                     LGLVIIDEQHRFGVAQRARMWSKSNNPPHILVMTATPIPRTLAMTLYGDLDISVIDEL
                     PPGRKPVKTILFSDASRLKVFGFMKQKIKEGRQIYVVYPLIKESETLDLKHLMDGYES
                     IVRDFPPPEYAVSIVHGKMRPADKDYEMQRFVKKQTQIMVSTTVIEVGVDVPNASVMV
                     IENAERFGLSQLHQLRGRVGRGADQSYCLLMTGWKVSQEARKRLETMVETSDGFKIAE
                     VDLQLRGPGDLQGTRQSGILDLKIADLIRDEKILKYARNLAFEILRDDPELAQEKNRP
                     LAMQLELLKKDFVDWSLIS"
     gene            4109..6571
                     /gene="pheT"
                     /locus_tag="PKZ74_09350"
     CDS             4109..6571
                     /gene="pheT"
                     /locus_tag="PKZ74_09350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPM93004.1"
                     /translation="MKISYNWLRRYVDLDLEPEELARKLTSSGLEVEDYEKTETVKGG
                     LKGVCVGLVVECEKHPNADKLSLTKVDTGIGRLLSIVCGAPNVRSGQKVPVATVGTIL
                     FQGDKSFEIKEAKIRGEFSEGMICAEDELGLGTSHEGIMVLDPEAEIGMPAAEYFHVS
                     EDYIFEIGLTPNRTDATSHLGVARDLVAVLNREAGERKYALKWPSVEEFAVDNHNRII
                     PVTVADATACPRYSGVTISGIKVAESPEWLKNLLNAAGIRPISNVVDVTNFVMLELGQ
                     PLHAFDASRIRGGKIVVRKASNDELFVTLDETERKLSGDDLMICNESEGMCIAGIFGG
                     AGSGVNENTTSIFLESACFSPQSVRKTSRRHGLQTDSSFRFERGADPNITVYALKRAA
                     LLIRELAGGEISSDIVDIYPEPVQPGHVIVSWKNISRLIGKEIDHSVIKSILADLGID
                     IASETMDGIELLIPTFKTDVTREADVTEEILRIYGYDNIEMPGHLRSSLSFSSKPDAE
                     EIRNLVSDMLSSRGFNEIMNNSLTRAKYAEECGWLKDEDSVRLMNPLSTDLNVMRQSL
                     LFGGLETIGYNQNRKSFDLRLFEFGRVYRIDRNRPGGRDTLAGYDEHESLSVFITGRM
                     HPESWRTSDIKADYFDLKSIVHLILERIGINPAHLQVSDDPGEPFEEGVEYSWNKVPV
                     IRFGLLKKKETKRFELKQDVYFADFNWDKVLGMLSVERVSYSGIPKFPEVRRDLALVV
                     DPAVSYADLEKAAFETEKKLLKNVSLFDVYQGEKIAAGKKSYALSFILRDDEKTLTDE
                     IIERTMNRILKAFEEKFKATLR"
     gene            6600..>7596
                     /locus_tag="PKZ74_09355"
     CDS             6600..>7596
                     /locus_tag="PKZ74_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93005.1"
                     /translation="MATSKEVVYSVSPAGEKFPLPLPGEFGKEYERLEKLAAKARKEG
                     KEVVVVMGVGFVGAVMAAIVADTKDKKGKNSKFVIGCQRPSARSYWKIPMLNKGLSPV
                     KAEDPEVDILISRCVKESKTLTATFNSDALKLADCVIVDVQCDYIKNSLGDMRDGEAD
                     MAALEATLKTIGQKIPEDCLVLIETTVAPGTTEFVAWPILKKEFSRRGLKKVPLLAHS
                     FERVMPGKEYVSSIRDFWRVCSGCDGEARKRVEKFLHEVLNTKEFPLTVMDRPIESET
                     TKIVENSYRATILAYLNEWSLFAERNGVDLIKVINAIKMRPTHSNMIFPGPGIGGY"
BASE COUNT         2141 a         1740 c         2034 g         1681 t
ORIGIN      
        1 tatgtgcgcc cggcatgggc gataactata gggtgaaaga cccgaacaca cttggcagta
       61 ggaagtgtta gccaaaggca agggtgtcca tcgtgaggtg gaatctgaag gaagccggcg
      121 gcaaaatccc ggcctgatga acaagaactg catataaggc ttatgaggga cggacgagct
      181 tgcataacaa agcaaattcc aatactgccc gaaccccttg gagtaaatgc agcaggtata
      241 tgggatgaaa gctatcgttc ttacccgggg agacctgacc cataggctgg ggatgaaata
      301 cggaaccggc aacctgtaca gcgatgtata gttgaatggt caggagtcag cagaggccat
      361 attacctggg cagacatgta tgcacaggga agggctgaac gtaaagatgt ctttaaattt
      421 tgaatgactt gtgagcaagg taaagaaagc agccaatgcc gacaataatc ggcactaccc
      481 accggaagcg ggagtggaac tccgaggtaa ggagggagag cggagctttg ccccagcgtc
      541 ggcagaagga gaagacggga ataaagtaaa cgccagtcag atgcttgaaa aagttctgga
      601 cagggaaaac ctgaatcttg cttacaagcg ggtaaagaag aacggcggca gccatggagt
      661 ggacgggatg aaggtggatg aacttttgcc ttacctgaaa cgacaggggg agagcctcaa
      721 gcgatcccta ctggaaggga agtaccgacc gcaaccggta cgcagggttg aaatacccaa
      781 gccggaaggc ggaataaggt tgttgggcat tccaacggta gtagaccgga tgatacaaca
      841 agcaatagcg caggtattgt ccccgatttt tgaggaagaa ttttctaagt acagttacgg
      901 cttccgaccc ggacgcggtg cacatcaggc catagaacag gcccggcagt acattaatga
      961 agggtataaa gtagtagtag atttggattt agagaagttc ttcgataggg ttaaccacga
     1021 taagctgatg tatcttcttg ccaaaagaat aacagacaag cgaatattga aacttatccg
     1081 ggcatacctt gaatcggggg taatgctggg cgggctgata agtccttcgg aagagggaac
     1141 acctcaagga gggccgataa gcccgctgct atccaatgta atgcttcacg aactagataa
     1201 ggaactggaa aggcgaggcc accggttctg ccgttatgcg gatgacagta atatctatgt
     1261 caaaagcaag aaagcgggga atcgggtgat ggaaagcgta agccagttta ttgaaaagcg
     1321 actgaaactg aaagtcaacc gggagaaaag tgcagttgac tacccaacaa agcggaagtt
     1381 cttaggatat agcttttact acaacagggg agtggcaagt ataagagtac atgccaaacc
     1441 actcagcaag ctaaaagcca agcttaagaa actgaccgga agaagcatcg ggataagcat
     1501 ggaagccaga acagacaaac tcaaccagac aatacgagga tgggttaatt attatcagtt
     1561 ggctgacatg ggcaaacatt gtcaaaagac cgatgaatgg ctgcgacgca gactacgcat
     1621 gtgctattgg aaacaatgga agaatatcgg ggcaagacat tacaatcttg tccgattagg
     1681 tatccctgat tccaaggcat gggagttcgc taatacgaga aatggccact ggcatactgc
     1741 caatagccct attcttgcca gtactcttac caatgagttc ttccgtaaac ttggccttac
     1801 aggtctttcc gatgtttatt taatatcttt atctttacga accgccgtat gccgaacggc
     1861 acgtacggtg gtgtgagagg acagtgaggg tacttatccc tcacttccta ctcgattttt
     1921 gtatatactc atattcaggc gcgatgacaa gagattttat tcaaaccccg gtggaattcc
     1981 tgaaaggagt gggccccaaa agggcggaat tgctcagatc agaactcaat attcatcact
     2041 tcggcgatct gctgaattat tacccgttcc ggtatattga ccgaagccgt atattcaaaa
     2101 tttcggagat cgaacccgat acagcctatg tgcagatcag gggaacagta accaatatac
     2161 gaatcatcgg tgaaaagaga gccagacgga tgtcggctct gctgcgcgat gataccgggg
     2221 aaattgaact ggtctggttc cagggaatta aatggatcga agggacgatc ctgcccaatg
     2281 tcgaatacat tgtgtttggg aaaccgacta tcttcaaccg gcgatataac atcgcccatc
     2341 cggaaattga acgcgccgaa gatttcttgc gaactgtgcc ccaaacactt cacggcatct
     2401 acagcacaac ggaaaagctt aaaaaccggg ggcttaacaa taaaaacctg gtgaagctaa
     2461 tccgggaact gatcattcag gccgaaggga aactccatga aaccctcccg gatgaaatta
     2521 tccgcaaact atcgctgctg ggtcgtgagg aagccctccg caatatacat tttccgttga
     2581 accagcaact tctgcaaaaa gcacaggccc ggcttaagtt tgaagagctc ttcttcattc
     2641 agctgggatt attgaaggat aagatgatca gacaacaaag gatcagcgga tttgtatttt
     2701 cccgcgttgc tgacaaagta aaccggtttt acagtgaaaa aatccctttt aatcttaccg
     2761 aagcacagaa aaaggttatt cgtgaaatcc ggaaagacct gggagccggt aaacagatga
     2821 accgcctgtt acagggtgat gtgggcagcg gtaaaacgct ggtggcgttg atggcgatgc
     2881 tgatagccat cgataacggg tttcagtcgt gcctgatggc cccgacggag atcctggcca
     2941 gtcaacacct cgccacactt acccgtatga ccgatggcct ggggatcggc atcggcctgc
     3001 tgaccggatc gactcccgct gcgaaaaggc gtgagatgca tgaaaaattg cagtcaggag
     3061 ccatggatat actgataggt acgcacgccc tgctggaaga cccggttgta tttaaaaacc
     3121 tgggcctggt gatcatcgat gagcagcacc gctttggagt cgcccagcgg gccaggatgt
     3181 ggagcaaaag caataacccg ccccatatcc tggtcatgac agccacgcct attccccgga
     3241 cactggctat gaccctttat ggtgatctcg atatatcggt catcgatgag ctgccacccg
     3301 gccggaaacc agtaaaaacg atcttattca gtgatgcctc caggctgaaa gtctttggtt
     3361 ttatgaagca gaaaatcaag gagggacggc agatctatgt cgtctatccg ttgatcaaag
     3421 aatcggaaac cctcgacctg aaacatctga tggatgggta cgaaagcatc gtccgtgact
     3481 ttccgccgcc ggaatatgcg gtgagtatcg ttcacggcaa aatgcgaccc gccgataagg
     3541 attacgagat gcaacgcttt gtaaagaagc agacccagat catggtatca acaacggtaa
     3601 tcgaggtggg agttgatgtc ccaaatgctt cggttatggt tattgaaaat gccgaacggt
     3661 ttggcttgtc acaactccac cagttgcgcg gcagggtcgg aaggggagcc gaccagtcat
     3721 actgcctgct gatgaccggc tggaaagtgt cgcaggaagc caggaaaagg ctggaaacaa
     3781 tggtcgagac cagcgatggt ttcaaaattg cagaggtcga tcttcagctg cgcggccccg
     3841 gcgatttgca aggtacccgg caaagcggta tcctcgatct gaaaattgca gacctcatcc
     3901 gtgatgaaaa aattctgaaa tacgcccgga acctcgcctt tgagatcctc cgggatgacc
     3961 ctgagctggc acaggaaaaa aacagacccc tggccatgca actggaactt ctgaaaaagg
     4021 atttcgtcga ctggagcctc atcagttaat agtaagcgcc cgaaaagcaa ttttcttaac
     4081 tttgccccga atcgttactt aatccggtat gaaaatatcc tataactggc tccggcgata
     4141 tgtcgatctc gacctggaac cggaagaact ggccagaaaa ctgacttctt caggccttga
     4201 agtggaagat tatgaaaaga ccgaaacggt aaaaggcggt ctgaaaggcg tatgtgtcgg
     4261 gctggtggtc gaatgtgaga aacaccctaa cgcggataaa ctgagcctga ccaaagtaga
     4321 tacaggtatt ggaaggctgc tgtcgatcgt ttgcggggcg cccaatgtca ggtctggtca
     4381 gaaagtccca gtcgcaacag tcggtacgat ccttttccag ggtgataaat ctttcgagat
     4441 aaaggaagca aagatcaggg gcgagttctc tgaaggaatg atctgtgccg aagatgaact
     4501 gggcctgggt acatcccatg aagggattat ggtcctcgat ccggaggccg aaattggtat
     4561 gccggccgcc gaatattttc atgtttccga agattatatt ttcgaaatcg gactgacgcc
     4621 gaaccggacc gatgccactt cccacctggg cgtggcccgc gatctcgtgg ctgtgctcaa
     4681 ccgggaagcc ggtgaaagga aatatgcact gaaatggcca tccgtcgaag aatttgcggt
     4741 cgataaccac aatcggatca tcccggttac cgttgccgat gcaacagcct gcccgcggta
     4801 cagcggcgtt acgatttccg gcattaaggt cgctgaatcg cccgaatggt tgaaaaacct
     4861 gctgaatgca gccggtatcc gcccgatcag caatgtggtc gatgtgacca atttcgtgat
     4921 gctggagctg gggcagccgc tccatgcttt cgacgcatcc cggatcaggg gcggaaaaat
     4981 agtcgttcgc aaagcgtcga acgatgaatt gtttgttact ctcgatgaaa ccgaaagaaa
     5041 actctccgga gatgacctga tgatctgcaa tgagtctgaa gggatgtgca tcgccgggat
     5101 ttttggaggc gccggatcgg gggtcaatga gaataccacc tccattttcc tggaaagcgc
     5161 ctgcttcagc ccgcagagcg tgcggaaaac ttcacgccgc catggccttc agaccgattc
     5221 gtcattccgc tttgaaagag gtgccgatcc caacattact gtctacgcac tgaaaagggc
     5281 cgccctcctt atccgggaac tggcaggcgg agaaatatct tccgatatcg ttgatattta
     5341 cccggaaccg gttcaacctg gccatgtaat agtgtcgtgg aaaaatattt cccgtcttat
     5401 cggaaaggag atcgaccatt ccgtgattaa atccatcctg gccgacctgg gcatcgacat
     5461 agccagtgaa acaatggatg gaatagaact tctcatcccg acttttaaaa ccgatgttac
     5521 ccgtgaagcg gatgtgaccg aagaaatcct ccgtatttac ggttatgaca atatagaaat
     5581 gcccgggcac ctccgatcat ctttatcatt ttccagtaaa ccggatgccg aagaaattcg
     5641 caacctggta tcggatatgc tttccagccg gggattcaat gaaatcatga ataattctct
     5701 gacccgggcg aaatatgccg aggagtgcgg gtggctgaaa gatgaagaca gcgtcaggct
     5761 tatgaatccg ctcagcaccg acctgaatgt catgcgccag tcgctccttt ttggcggact
     5821 ggagaccatc ggttacaacc agaaccggaa atcgttcgac ctgcggttat tcgagtttgg
     5881 cagggtttac cggatcgacc ggaacaggcc cggaggccgg gatacactgg ccggatacga
     5941 tgaacatgag tccctgtcgg tttttatcac cgggcggatg catccggaaa gctggcggac
     6001 cagcgatata aaggcagatt atttcgacct gaaatcaatc gtgcacctga ttttggaaag
     6061 gatcggcatc aatccggccc acctgcaggt ttcagacgat cccggagagc cgtttgagga
     6121 aggggtggaa tattcctgga ataaagtgcc cgttatccgt ttcggattat tgaaaaagaa
     6181 ggaaacgaaa cggtttgaac tgaaacagga tgtctatttt gccgatttta actgggataa
     6241 agtactggga atgttatccg ttgaacgcgt ttcgtattcc ggtatcccaa agtttcccga
     6301 ggtcaggcgc gacctggcac ttgtcgtaga cccggccgtg tcttatgccg atcttgaaaa
     6361 agcggcattt gaaaccgaaa agaaacttct taaaaatgtc agtttgtttg atgtttacca
     6421 gggtgagaaa attgcagccg gtaagaaatc ctatgcatta agcttcattc tcagggatga
     6481 tgaaaagacc ctgacggatg aaatcattga acggacgatg aacagaattc ttaaagcttt
     6541 tgaagaaaaa ttcaaggcaa ccctacgata attcattcaa aaacaaaatt ttactattta
     6601 tggcaacatc aaaagaagtg gtttattccg tcagtccggc aggtgagaaa tttccccttc
     6661 ctttacctgg agagtttggc aaagagtatg agcgactgga gaaactggct gctaaagcca
     6721 gaaaagaagg aaaagaggtt gtggtggtga tgggggtcgg atttgtgggg gccgtaatgg
     6781 cagccattgt agccgataca aaagataaaa aaggaaaaaa ttcgaagttc gtcatcggtt
     6841 gccagcgtcc cagtgcccgg agctactgga aaatcccgat gctgaacaaa ggattgtcac
     6901 cggtgaaagc ggaagacccc gaggtggata tcctgatcag ccggtgcgtg aaagagagca
     6961 aaacgctgac cgccaccttc aacagcgatg ccctgaaact tgccgactgt gtgatcgtcg
     7021 atgttcaatg cgattatatt aagaattcac ttggcgatat gcgtgatggc gaagccgata
     7081 tggccgccct ggaagctacc ctgaagacaa tcgggcagaa aatacccgag gattgccttg
     7141 tgctgatcga aacgacggtt gcgcccggga ctaccgagtt tgtggcctgg ccgatcctga
     7201 aaaaggagtt ttcgcgacgc ggtctgaaga aggtgccttt gctggcccac agcttcgaac
     7261 gggtcatgcc gggcaaggaa tacgtttcca gcatccgcga tttctggcgg gtttgttcgg
     7321 gttgcgatgg ggaggcccgc aagcgtgtcg agaaattcct ccatgaagtt ttgaatacaa
     7381 aagaatttcc tcttaccgtg atggaccggc cgatcgagtc ggaaaccacc aaaatcgttg
     7441 agaattctta ccgggcaacc attctcgctt acctgaacga gtggagcctc tttgccgaac
     7501 gcaatggcgt cgatcttata aaagtcatca atgccatcaa aatgcggccg acccacagca
     7561 atatgatctt tccgggaccc ggtatcggcg gctact
//
LOCUS       DAOPNH010000165         7594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_10900_length_7594_cov_4.940443, whole genome shotgun sequence.
ACCESSION   DAOPNH010000165 DAOPNH010000000
VERSION     DAOPNH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10900_length_7594_cov_4.940443"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PKZ74_09360"
     CDS             <1..1460
                     /locus_tag="PKZ74_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93006.1"
                     /translation="DGASFIPESGTVNNYGKVMAAIGRPIIFKKDPAASESQRVVVNT
                     TTLGTIADAFYVGTPISPDFTADSANWHIFEGIDITGGNGTKGLHIQCSSQNVFAKGK
                     IYSSGTSGTNNLGVIISGNSSRGARFNIIQNNEIYNTPYQGILIGYTNNSASYNFSHN
                     NHVLDNNMYITGSTFSARFNNAVKIQQFNKNNVIEGNNFHDIPIYTAGEAAVAVVTKA
                     DSTLIYNNIFRNIGKSASNSGVNAAIMLDSTIYKAHVYNNIIYNDDTVTNAVYAFRVY
                     GRRHVGSKLAFNTVYKIDNAFYLQDNNANGAAIDFGIHNNIISPTVGYFNNVGTTGRF
                     TVTYNLFRISPGTPYASGTGNIFGDPLFIDPNGSSMYGLMLTPTSPAMDTGTVITNLT
                     RDYIGDLRGSPEPTLGAFENTMTATWNGSVSTDWNNVNNWTYKILPKNYSPVVIPNVT
                     NDPVVSTGNATCNSISLSPGATLRVSPPRTLTVNY"
     gene            1553..2902
                     /locus_tag="PKZ74_09365"
     CDS             1553..2902
                     /locus_tag="PKZ74_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM93007.1"
                     /translation="MSKNKRKYLLILGLVLLLIPAYFLFASANPKTAERFPFLVFLLA
                     VDFYFWYSIKDQFKKLNKVLKTILSVIYWLPLGAFVVLLALTSVHKYNLTHPALVSYI
                     FGFVMVIYLAKLITVIFFLFADIFRISLFTVRHARAKKTGRPIDHGTHMSRGKFIRTM
                     GLATGGLFFSGMLIGMVRWAHDFRVRRENLFLPNLPKSFNGLRIVQISDLHLGSWASA
                     DPIAEAADIINDLDPDVVLFTGDLVNYSTNEAYRFRNELGRIRARHGVFAILGNHDYG
                     DYVTWPDETAKLQNLESLYQFFDEIGWRLLRNENQVLERNGDKIAIIGVENWSASKRF
                     PKLGNMTKAISGTENIPVKILMSHDPTHWEKEISHKFNDIDLTLSGHTHGFQFGVELK
                     NFRWSFAQYVYKYWAGLYESTHKGKKQYLYVNRGLGMIGYPGRVGILPEITLITLNS"
     gene            2966..4885
                     /locus_tag="PKZ74_09370"
     CDS             2966..4885
                     /locus_tag="PKZ74_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93008.1"
                     /translation="MKKFYPFLLVLATPVVLLLMSNNNGSPGGKSGSIGDGGATCTQC
                     HTGGSATTVSGWITTNVPADGYTPGQTYTITATGTHTGVVKFGFELTVESSTGAKVGT
                     LQLTEPTRTKFTNANQAVTHTAAGNVPSGNTNSWTMNWVAPTGVQGNIGSYAAFNAAN
                     GNGNNSGDVIYKSSTFISQYVPPPTLVSIVPDEAEQGQTVQTTITGSNTQFSGSPSVS
                     LSFSNNPNETISASNVVVVNATVIQAQFAVPGNASVGLWDLHVNLLSLDNAFTVIQAE
                     PSIVFMEPNFAYQGDSFTGTIYGENTTWSGTPSVTLTHVNNPNEIITGTNVTVVSPTQ
                     LTADFAIAEAGVIGNYNVNVDDLMQAAGFTVLAALVPEIAGISPVEGEQGTMVTTTIT
                     AENTDFGQPAPVVSLTLHGNPGESIPATSVTVINGTTLEADFDIPYEATPGLYDLQVD
                     DLTLVNAFTVIDVVPFLVSISPNTADQGETVVTTITAADSRFTLDEPSVSLSFSGDPL
                     ETIAASGVTVISNFVVEAEFIIPSNASVGTWDVHADEMTLSGAFTIDQAIGIADNLAG
                     MIRIYPNPADQKFFVENSRGAEITIFRTSGEQVLSLVAGNIRQEVNIETLTQGLYLVN
                     IRMNGMVKTEKLLVK"
     gene            complement(4889..5473)
                     /locus_tag="PKZ74_09375"
     CDS             complement(4889..5473)
                     /locus_tag="PKZ74_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93009.1"
                     /translation="MRPEDGGTPGFLQAIKAALAIGLKEKMGTGNIKKRESELLSVAF
                     AGFDRIPELHLLADENRERLGIISFYIENIHFNLAVKLLNDRFGIQVRGGCACAGTYG
                     HYLLDVSYDKSHLITSLISSGDLSLKPGWIRLSLHPTMTRGELEYILDAIGQVQKNHK
                     KWCDDYVYNRHTNEFRHRNEPEDKTVLVREWFEL"
     gene            complement(5403..6350)
                     /locus_tag="PKZ74_09380"
     CDS             complement(5403..6350)
                     /locus_tag="PKZ74_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93010.1"
                     /translation="MENYFKPFRENIIGIHDHFNSPYGRVPVVYADWIASGRLYRPIE
                     ERIIETLGPFVGNTHTETSETGTLMTNAYHFAHQKIKEHVNASADDVIITAGFGMTAV
                     VNKLQRMLGLKSCGQGSKHNCIDESDRPVVFITHMEHHSNHTSWFETMSDVVQVPPAN
                     DLMIDLNRLRELLEQYKERKIKIGTFTACSNVTGIATPYHQMARLMHEYGGYCFVDFA
                     ASAPYVDIDMHPEDPMEKLDAIFFSPHKFLGGPGSSGVLIFDRNLYSGHRPPDQPGGG
                     TVDWTNPWGEYKYIDDIEARGWRDTRVSPGYQSGIGNRA"
     gene            complement(6414..>7594)
                     /locus_tag="PKZ74_09385"
     CDS             complement(6414..>7594)
                     /locus_tag="PKZ74_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93011.1"
                     /translation="KIKEIDLILADIAAKEAAAKELAAKEAAEKEAAAKELAAKEAAA
                     RELAAKEAAEKEAAAKELAAKEAAEKEAAAKELAAKEAAEKEAAAKELAAKEAAEREA
                     AAKEQINREYLLALETAANYLKDGRLEESRTAYLEASAIKPGEEMPKQQIREIDRQIK
                     ERIAELDLAYQVAVSKGDNFFGLQDFESARMQFTRALELKPNEAYPAEQLRLADMEIA
                     KKQKLIQEEYDRTIIEADKYFASKTYDSALELFRKAAALKPDMDYPQEMAGRILKLLS
                     ERSIVQINTLPVAIASNTTHKFSFTPVPVKDRKSNYIYFKARNISGNEFKLIISYGKD
                     QAKNGGVVVKVPAGEDLIDYLVRISAQYKWFSEDNNWITVYPEGGECELSLMQVSYSD
                     "
BASE COUNT         1880 a         1941 c         1744 g         2029 t
ORIGIN      
        1 tcgacggggc ttccttcatt cctgaatccg gaacggtaaa taattatggc aaggtaatgg
       61 ccgctattgg ccgcccgatc attttcaaga aggatccggc tgcttctgag agccagcgtg
      121 tagtggtcaa tacgacaact ctcggtacaa ttgctgacgc cttttatgtt ggaacgccta
      181 tcagcccgga tttcacagcc gattcggcca actggcatat tttcgaggga attgatatta
      241 caggggggaa tggcaccaaa gggcttcata ttcaatgctc atcccagaat gtttttgcca
      301 aaggaaagat atattcatcg ggaacttccg gtaccaacaa cctgggggtg atcatctccg
      361 gtaacagcag ccggggagcc cggttcaaca ttatccagaa caacgagatc tacaatacac
      421 cataccaggg aatactgatc ggttatacta ataactcagc atcttataac ttcagtcata
      481 acaatcatgt gctggataat aatatgtaca ttacgggaag tacgttctca gccaggttta
      541 ataatgctgt aaaaattcaa cagttcaata aaaataacgt cattgaagga aataatttcc
      601 atgatatccc tatttatacc gccggtgaag ctgctgttgc cgtagtcacc aaggccgaca
      661 gtacactgat ctacaataat atcttcagaa atatcgggaa atctgcgtct aattcaggcg
      721 ttaatgccgc catcatgctc gacagtacca tttataaggc tcatgtttac aataatatta
      781 tttataatga tgatacggta accaatgcgg tttatgcatt ccgtgtctac ggccggcggc
      841 acgtcgggag caagctggct ttcaatacag tatataaaat tgataatgct ttttacctgc
      901 aggataataa tgccaatggc gctgccatcg atttcggcat acataataat attatcagtc
      961 ctaccgtcgg ttatttcaat aatgtcggga ccacgggccg gtttaccgtg acatacaact
     1021 tattccgcat atctcccgga acgccctatg cttcaggaac cgggaatatt ttcggcgatc
     1081 ctttattcat cgatcccaac ggcagcagta tgtacggcct gatgctcacc ccgacttcgc
     1141 cggccatgga caccggtaca gtgatcacca acctgacacg cgactatatc ggcgatctca
     1201 ggggaagccc ggaacctaca ctcggcgcat ttgaaaatac tatgaccgcc acctggaacg
     1261 gttcggtcag caccgattgg aataatgtga ataactggac ttacaagatt ctcccaaaga
     1321 actattctcc tgtcgttatt ccgaatgtta ctaatgatcc ggtcgtttca accggcaatg
     1381 ctacatgtaa ttccatcagc ctttcacccg gagcgacgtt gcgtgtgtct cccccaagaa
     1441 cccttaccgt caattattaa atattcttaa atttattcaa atcggataaa tatttaaaat
     1501 tttcaataca tactttttat acttttgcgc cctcaattaa cggataaggg ttttgtcgaa
     1561 aaataaaagg aagtatttac tgattttggg gctggttctg ctcctgattc cggcttattt
     1621 tctttttgcc agcgccaacc cgaaaacggc cgaacgattc ccgttcctgg tctttctcct
     1681 ggccgtcgat ttctacttct ggtattcgat caaagatcag tttaaaaaac tgaataaagt
     1741 actgaaaacc atcctgtcgg tgatatactg gttaccactg ggagcattcg tcgtcctgct
     1801 ggcgctcacg tctgttcata aatataatct cacacatccg gcgctggttt cgtacatctt
     1861 tggttttgtt atggttattt acctggcaaa actgatcacg gtgatctttt tcctttttgc
     1921 cgatatattc aggatcagct tgtttactgt caggcatgcc agggcaaaga agaccggccg
     1981 gccgattgat cacgggaccc atatgagccg gggaaaattt atccggacga tggggctggc
     2041 tacgggaggg ctttttttta gcggtatgct gatcgggatg gtgcgctggg cccacgactt
     2101 cagggttcgc cgtgaaaacc tcttcctgcc gaacctgccc aaatccttca acggtcttcg
     2161 aatcgtccag atctcggacc tgcatctggg aagctgggca tcggccgacc caattgccga
     2221 agctgccgat attatcaacg acctggatcc cgacgtggtt ttgtttacgg gcgacctggt
     2281 caactacagc accaatgaag cctaccggtt ccgcaatgag ctgggccgga tcagggcccg
     2341 gcacggcgtt tttgccattc tgggcaatca cgattatggc gattatgtta cctggccgga
     2401 tgaaactgcc aaattacaga acctggaatc tttatatcaa tttttcgacg agatcggctg
     2461 gcgtctgctc cggaatgaaa accaggtact ggaaagaaat ggagataaaa ttgccatcat
     2521 cggggtggaa aactggagcg ccagcaaacg gtttcccaaa ttgggtaata tgaccaaagc
     2581 aatctcaggt accgaaaata ttccggtaaa gatcctgatg tcgcacgatc cgacccattg
     2641 ggaaaaagag atcagccata aattcaacga catcgatttg accctttcgg gtcacaccca
     2701 tggttttcag tttggcgtcg aattgaaaaa cttccggtgg agttttgccc agtatgttta
     2761 caaatactgg gcgggacttt atgaatcgac ccataaagga aaaaaacaat acctatatgt
     2821 caaccgcggc cttggaatga ttggctatcc cgggcgtgtg ggtatcctcc ctgagattac
     2881 cctgattaca ctgaattctt aaatatttgt taaatattga acttttttta tatgatgttg
     2941 tttgcactta aaaatgtaaa caactatgaa aaaattttac ccctttttat tggtattggc
     3001 aaccccggta gtccttctcc tgatgagtaa caacaatggc tcgcccggcg gtaagtcggg
     3061 atcgatcggc gacggaggag ctacctgtac ccagtgccac accggtggta gtgcaacaac
     3121 tgtctcaggc tggatcacga ccaatgttcc ggcggatggt tacactcccg gacaaaccta
     3181 cacaattacg gcaacaggta cacataccgg cgttgtaaaa tttggctttg agctgaccgt
     3241 agaaagcagc accggagcta aagtaggaac cctgcaactg actgagccga cccgcacaaa
     3301 gttcactaat gcgaatcaag ccgtaaccca cacagcggca ggaaatgttc cgtcaggaaa
     3361 taccaactca tggacaatga actgggtggc tccgacgggg gttcagggca atatcggcag
     3421 ctatgccgca ttcaatgcag ccaacggaaa tgggaacaat tcaggtgatg tgatttacaa
     3481 atcaagcacc tttatttcac aatatgtacc gcctccaact ctcgtaagca tcgtccccga
     3541 tgaggccgaa caagggcaaa cggttcagac tacaattacc ggtagcaata ctcaattttc
     3601 agggtcgcca tctgtttccc tctccttcag caataatccc aacgaaacta tttccgcttc
     3661 aaacgtagtg gttgtcaatg caactgttat tcaggcacaa tttgcagtcc cgggaaatgc
     3721 cagcgtcggg ctttgggatc tgcacgtaaa cctgctttca ctcgataacg cgtttacggt
     3781 catccaggcc gaaccctcta ttgttttcat ggagcccaac tttgcttacc agggtgattc
     3841 attcacaggt acaatctatg gcgaaaacac cacctggagc ggaacaccat cggttaccct
     3901 gactcatgtc aataatccga acgagatcat taccggtacg aatgtcactg tggtcagccc
     3961 gacccagctg accgccgact ttgcaattgc tgaggcaggt gtgatcggaa actataatgt
     4021 caacgtcgat gacctcatgc aagctgcagg tttcaccgtt ctcgccgctc tcgttccgga
     4081 aattgccggt atcagtccgg ttgaaggtga gcagggaacg atggtcacca ccacgataac
     4141 cgctgaaaat accgatttcg gacaaccggc tccggtggtt agcctgacgt tgcatggcaa
     4201 tccgggcgaa agtatcccgg caaccagcgt taccgttatt aatggaacca ccctggaagc
     4261 cgatttcgat attccttacg aagcaacccc ggggctgtat gaccttcagg tcgacgatct
     4321 gacgctggtt aatgcgttta ccgttatcga cgtggtgcct ttcctggtca gtatttctcc
     4381 caatacagcc gatcaggggg aaaccgtcgt gacaacgatc acggcagccg acagccgatt
     4441 taccctggat gaaccatctg tttctctgag tttttcgggt gatccgcttg aaaccattgc
     4501 tgcttcgggc gttacagtta tcagtaattt cgtcgtcgaa gctgaattca ttattccgtc
     4561 aaatgcatct gtcggaacat gggatgtcca tgccgatgaa atgaccctca gcggagcgtt
     4621 taccatcgac caggccatcg ggattgccga taatcttgcg ggcatgatcc gaatctatcc
     4681 gaatccggcc gatcagaagt tcttcgttga aaacagccgc ggagctgaaa ttaccatctt
     4741 ccgcactagc ggcgaacagg tgttgagcct ggttgccggc aatatccggc aggaagtcaa
     4801 tattgaaact ctgacacagg gattgtacct ggtcaatata aggatgaatg ggatggtgaa
     4861 aacggagaaa ctgctcgtaa agtaaaaatt acagttcgaa ccattccctg acaagaacgg
     4921 ttttgtcctc aggttcattc cggtgcctga actcattggt gtggcggtta taaacataat
     4981 cgtcacacca ttttttatgg tttttctgaa cctgcccgat tgcatccaga atatactcca
     5041 gctcgccacg ggtcatcgtc ggatgcaggg agagcctgat ccatccgggc ttcagggata
     5101 agtcgcctga gctgatgagg ctggtgatca gatgggattt atcataggaa acatccagga
     5161 ggtaatgccc gtaagtgcct gcacaggcgc agcctccgcg cacctggata ccgaaccggt
     5221 cgttcagcag tttgacggcc aggttgaaat gaatattctc aatgtaaaaa gagatgattc
     5281 cgagcctctc ccggttctcg tcggccagca ggtgaagctc gggaatacgg tcaaaacctg
     5341 caaaagccac agataacagt tcgctttccc tttttttgat atttcctgtg cccatctttt
     5401 ctttaagccc gattgccaat gccgctttga tagcctggag aaacccgggt gtcccgccat
     5461 cctcgggcct caatatcgtc gatgtattta tattcccccc agggatttgt ccagtcgacc
     5521 gtccctccac ccggctgatc gggcggacgg tgaccgctgt aaagattcct gtcgaaaatc
     5581 aagacgcctg aacttcccgg gccaccgaga aatttatgcg gtgaaaagaa aatggcatcc
     5641 agcttctcca tcggatcttc cgggtgcata tcaatatcga cataaggcgc tgatgcagca
     5701 aaatccacaa aacaatagcc gccgtattcg tgcataagcc tggccatctg gtgatatgga
     5761 gtggcgatgc ccgtcacatt ggagcaggca gtaaatgttc cgatcttgat tttccgttct
     5821 ttatattgct ccagtaactc ccgtaaccgg ttcaaatcga tcatcaggtc gttggctggc
     5881 ggaacctgaa cgacgtccga catggtctcg aaccaggaag tatgattgga atgatgctcc
     5941 atatgggtga tgaaaactac cggccggtcc gattcatcga tgcagttgtg cttcgatccc
     6001 tggccgcagc tttttaaccc gagcatccgt tgcaatttat tcacaaccgc tgtcattccg
     6061 aatccagccg tgatgatcac atcatctgcc gatgcattga catgctcctt gatcttctga
     6121 tgggcaaaat ggtaggcatt ggtcattaat gtgccggttt cactggtctc ggtgtgcgta
     6181 ttaccgacaa aaggcccaag tgtttctatg attcgctctt cgatcggacg gtataagcgc
     6241 ccggaggcta tccagtcggc ataaactacc ggaacccggc catatggtga gttaaaatga
     6301 tcatggatac caatgatatt ctcccggaaa ggcttgaaat agttttccat aaatggttga
     6361 aattgcgaca aaagtaaaaa aattgcgcac ttttcagaac agccggccta atcttaatcg
     6421 ctgtaagaaa cctgcataag cgataactcg cattcaccgc cttccggata gaccgtaatc
     6481 cagttgttat cctcgctgaa ccatttgtac tgtgcgctga tcctgaccag gtaatcaatc
     6541 aaatcttccc cggcagggac tttcacgacc acacctccgt tcttggcctg gtctttcccg
     6601 taactgatga tcaacttaaa ctcattcccg gaaatattgc gggctttaaa ataaatgtag
     6661 ttgcttttgc ggtctttaac cggtacaggc gtaaaactga atttgtgagt ggtattactt
     6721 gcaatcgcca ccggaagggt attgatctgc acgatagatc tttcgctcag taatttaagg
     6781 atccggccgg ccatttcctg cgggtaatcc atgtccggtt tcaatgcagc ggccttcctg
     6841 aagagctcca gtgcactgtc gtatgtcttg gaggcaaaat atttatcggc ttcaatgatg
     6901 gtcctgtcgt actcctcctg gatgagcttc tgctttttgg caatttccat gtcagcaagc
     6961 cgtaactgct ctgcaggata agcttcgttg ggtttaagct ccagggcgcg ggtgaattgc
     7021 atcctggccg attcaaaatc ctggagtccg aagaaattat cacctttcga aacagctacc
     7081 tggtatgcca ggtcaagttc ggctatcctt tctttaattt gccggtcgat ctcccggatc
     7141 tgctgcttgg gcatttcttc cccaggttta atcgctgatg cttccaggta tgcggtcctc
     7201 gattcctcta atctcccatc ctttaagtaa ttagccgctg tctctaaggc cagaagatac
     7261 tccctgttga tctgttcttt cgctgcagct tctctctcgg cagcttcctt tgctgccagt
     7321 tctttcgccg ccgcttcctt ttcagccgcc tctttggctg ccagttcttt cgctgccgct
     7381 tccttctccg ccgcctcctt tgccgccagc tccttcgcag ccgcttcctt ttcagccgcc
     7441 tcctttgctg ccagttccct tgcagccgct tctttggctg ccagttcctt cgctgccgct
     7501 tctttttctg ccgcttcttt ggccgccagc tccttcgccg ccgcttcttt tgctgcgata
     7561 tcagccagga tcaggtcgat ctctttgatc ttgc
//
LOCUS       DAOPNH010000166         7536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11025_length_7536_cov_6.822083, whole genome shotgun sequence.
ACCESSION   DAOPNH010000166 DAOPNH010000000
VERSION     DAOPNH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11025_length_7536_cov_6.822083"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKZ74_09390"
     CDS             complement(<1..462)
                     /locus_tag="PKZ74_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93012.1"
                     /translation="MINSGAFGWAMQCSMGGLLFIYGGYKLATTRLKQNSLVLFSLSG
                     PLFLIESIFMFKDGLYHLLPNILILLFGTGIGIGLKILFLNGRHNIFYVSLSLGLLFS
                     LLCYFITLNWCEYVFTIKDDYRINNFELNFRTLDGHEMNNSSFMGKLTVLYL"
     gene            complement(914..1579)
                     /locus_tag="PKZ74_09395"
     CDS             complement(914..1579)
                     /locus_tag="PKZ74_09395"
                     /inference="COORDINATES: protein motif:HMM:NF033852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin fulvocin C-related protein"
                     /protein_id="HPM93013.1"
                     /translation="MKAKLRIRMEVFLTCSILVSILTNCSQESKYSCDEEVNQWVKYH
                     LKDLQDITRTELAGYPFNYQVAIFRSLDAYKKSEIWIEKVDSIQLYNWPQNLYPYIDS
                     LKTRIQADFFEAENATSSKAYCSTLLSYILSLQGIDTSQVVCSFYTIMTISEIEQGVY
                     AQQPGPQNCVCNWDITCSILNAGLCEDGLDGCMATDDGCGFLYMYSCEGDCTDMVVKP
                     DEN"
     gene            complement(1901..2356)
                     /locus_tag="PKZ74_09400"
     CDS             complement(1901..2356)
                     /locus_tag="PKZ74_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPM93014.1"
                     /translation="MDLENRINTDIKQAMMAKDSRKLEALRAIKAALLLAKTSKEAVN
                     GEISDEAGLKLLQKLVKQRHESAEMYTAGGRTDLAEVEVYQASVIEEYLPKQLGKEQI
                     RAGLEQIIRDTGASGLKDLGRVMGQASKLFAGKADNKVVAELVKELLGN"
     gene            complement(2377..4104)
                     /gene="ftsZ"
                     /locus_tag="PKZ74_09405"
     CDS             complement(2377..4104)
                     /gene="ftsZ"
                     /locus_tag="PKZ74_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPM93015.1"
                     /translation="MKEMIQFDMPKNQSSIIKVIGVGGGGSNAVNHMFRQGIKGVDFI
                     ICNTDAQAMESSPVPSKIQLGEKGLGAGSVPAVGREAALNRIEQIREILEKNTQMLFI
                     TAGMGGGTGTGAAPVIAQVARELGILTVGIVTIPFAFEGKKRRTQADEGLLELKKYVD
                     TLVIICNDKLRELCGDLKLSAAFDKADDVLTTAAKGIAEIITVTGYINVDFEDVKTVM
                     KESGKAIMGYAQAEGENRALDAVKGALSSPLLNDNDIEGASNVLLYITSGEEEISMDE
                     VTEITDYIQQEAKSQAEIIWGNGYDDKLGKSISITLIATGFEPQKKGDAQDNRVVHTL
                     GNQTVAATPMPEMQAIDDSLMNARTFELLRKPAMEETAENEAVEEESFSEPETAEATG
                     EESEMETVSVEAPVMEEKQEMNIPEPEDRTFVFEFQQPEEISREEQEVVKPEYQPIVN
                     RIGRDDQPERPVSGKQDEIRPFEVYVKKPGGETFRDTEKVFTRPSVDLRSQERIERLR
                     NLSLKMHQESVIEELENEPAYVRRNVELKNFTSSGEAHVSKYSLYEDPETRNTEIRSG
                     NSYLHDNVD"
     gene            complement(4196..5605)
                     /gene="ftsA"
                     /locus_tag="PKZ74_09410"
     CDS             complement(4196..5605)
                     /gene="ftsA"
                     /locus_tag="PKZ74_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPM93016.1"
                     /translation="MNKSDIIVGLDIGTTKIAAIVGRRNEHGKIEILGYGNTQSIGVK
                     RGVVSNIENTVQSIRTAVEMAEKSSGVEIRYVNVGIAGQHIKSLQHKGTIIRRDIENE
                     ISQQDIDTLHQNMFNLNMNPGEEIIDVIAQEYIVDGEEDVKQPIGMLGNTLEANFHII
                     IGQTAAAKNIYKCVRKAGLEVVDLILEPIASADAVLGEEEKEAGVVLVDIGGGTTDIA
                     IFQDGIIRHTSVIPFGGEILTEDIKEGCSIIKKHAEELKIRFGSALASENREDEVVAI
                     PGLRGRQPKEITLKNLASIIQARMEEIIEHVYYEIKNSGYEKKLIAGIVLTGGGSLLK
                     HLAQLTEFMTGMDTRIGYPNEHLAPETLDEMASPMYATGIGLVIQGIERQIREQVRSG
                     NKMQDKAPQSREQELPAPAPAPAPSQEQKPEPLPETVDDKPELQEEEEIEEEKPVEKK
                     PRTNGFLKRIQDWFEGDEV"
     gene            complement(5602..6393)
                     /locus_tag="PKZ74_09415"
     CDS             complement(5602..6393)
                     /locus_tag="PKZ74_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93017.1"
                     /translation="MKKLLNILFLIFLMAAVIILPAFTDLEHHRNVYREFTVEVLNQT
                     DKSLITTSEIHELVRKNFGEIKGSPVSIIDIDKMEKLVDGNPYVSACEVYKTMDCELI
                     MKARVREPLVRIANRQGEQFYLDLNGCAMPLNPAHPSHVTLAGGNISERLMSIDKSEK
                     PLDKFDRSSVLHQIYPVAYAISRDEFLESFIDQIYIHENGEIELVPRVGSQIILLGNG
                     EDAAEKLENLKVFYRKVMRNIDWNTYKTINLKYKNQVVCIKNPTL"
     gene            complement(6390..>7536)
                     /locus_tag="PKZ74_09420"
     CDS             complement(6390..>7536)
                     /locus_tag="PKZ74_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM93018.1"
                     /translation="IVYTPAVPESLNEYKFLASSGIRMIKRALAVGELTEGRTTVAVA
                     GTHGKTSISSLIAHILIEAGRPVTALIGGIARNYDSNMVTSGKEEIMVVEADEYDRSF
                     LYLHPDIAVISAIDADHLDIYGSTGSLQQSFSEFAAHIKPGGMLALREGIEVDLKSTF
                     ETVTYSVEGNADISALNPVIREGRQRFDMSLKGRMVRDVALRIPGRHNVENAMAAAAV
                     CVKLGVSPEQIAAAVSTFTGVTRRYDVRLDEPGPVYIDDYAHHPRELEAFISAVRMMY
                     PGKKLTGMFQPHLYSRTRDLADGFAQSLDLLDEAWLLDIYPARELPIPGVSSEMILEK
                     MKLPHKKIVSREQALQSLTENRPEIFLTIGAGDIDLLVGKIEKILRA"
BASE COUNT         1749 a         1920 c         1716 g         2151 t
ORIGIN      
        1 taaatatagg acggttaatt tacccatgaa actactattg ttcatttcgt gcccgtccag
       61 ggttctaaag tttaattcga aattattaat tctgtaatca tcttttatag taaatacata
      121 ttcacaccaa ttaagagtga tgaagtaaca taataatgaa aataatagcc ccaatgataa
      181 agacacataa aatatattat gacgtccatt taaaaataat atctttaggc ctattcctat
      241 tcctgttcca aaaagtaaaa taaggatatt tggcaataaa tggtagagac catccttaaa
      301 cataaagatc gattcaatta aaaataatgg accacttaaa gaaaataata ctaagctatt
      361 ttgcttaagt ctcgttgtag ctagtttgta tccaccatag ataaatagca atcctcccat
      421 actacattgc atggcccaac cgaaggctcc cgaattaatc atcaataaat taattaagag
      481 tataagatag ataaatctac ccagtgttga tcgtttgatt ttatacctaa ggaagtaacc
      541 tgaacaaaag gaaagaaaaa aagctggtat acaaattaat ataaaaggta aaattttaaa
      601 aatataagta atttcaagtt tgtgatgata ctcgacgaaa acaaaaaaac aaataataaa
      661 aattaaaaag aagatattag ttatactcct gactattgat agttgaagac ttgcatctga
      721 tggcttccaa taccctgtta ttagataaat gatacatcca ttataaatga agaaattaat
      781 ttcccttgta tttattaaaa aagaaggaat taaaaccagg aaagggaata gcgacatgaa
      841 gtatttatta ccacgcaacg tgataatgat tgtcttaatt cccttaatat tgagatatct
      901 tttataaaag atgttaattt tcatcaggtt tgacaaccat gtctgtacaa tcaccttcac
      961 aagagtacat atagaggaat ccgcatccgt catcagttgc catacatcca tccagaccat
     1021 cttcacacag tcctgcatta agtatcgaac aagttatatc ccaattacaa acacaattct
     1081 gaggtcccgg ctgctgagca taaacgcctt gttctatttc gctgattgtc attattgtgt
     1141 aaaaactgca gactacctga cttgtatcaa taccttgtaa acttaatatg tagcttagta
     1201 atgtactaca ataagctttt gatgaagtcg cattttctgc ttcgaaaaag tctgcttgga
     1261 ttctcgtttt caagctatca atataaggat aaagattttg aggccaatta tataattgaa
     1321 tactatcaac cttttcaatc catatctcag attttttata agcatcaagc gatcgaaata
     1381 tcgctacctg ataattaaaa ggataacctg ctagttcagt tcgggtaatg tcttgcaagt
     1441 cctttaaatg atatttaacc cattgattaa cttcctcatc acaactatat tttgattctt
     1501 gtgaacaatt ggttaaaatg gaaacaagaa tgctacaggt taaaaacact tccattctga
     1561 tccttaattt tgctttcatg gctttagaat ttattgaata atataatact tcttgtttca
     1621 aacgaagaat agcttggaca tttatgatct aaaggaaata ttccccttca agcaacttac
     1681 ttgttcagaa aataattagt tgcatcaaat ataagaaatt attatcctta aatcaataga
     1741 atgagcagat ttagaataag tttaaataaa ccgtgggggg atgttaaatg ggttggattc
     1801 gtggaaatac cgaaattaaa aaggctgcca tcccagcagc ctttttttgt caaacccaat
     1861 catgaaagaa cacatctttg cgatatgctt atcttaaata tcagtttccc agcagttcct
     1921 ttacaagttc cgccaccact ttattgtcgg ccttcccggc aaacagcttc gacgcctgtc
     1981 ccatcacacg cccgagatcc ttcagcccgg atgcgccggt atcccggatg atctgctcca
     2041 aaccggccct gatctgttcc tttcccaatt gtttaggcag atactcctca ataacggatg
     2101 cctggtacac ctctacctcc gccaggtcgg tgcgcccgcc ggccgtgtac atctcagccg
     2161 actcgtgccg ctgtttgacc agtttctgta agagcttcag gcccgcttca tccgaaattt
     2221 ccccgttcac cgcctctttg ctcgtctttg ccagcagcaa ggccgctttg atcgcccgca
     2281 aagcctccag cttccggcta tctttagcca tcatcgcctg cttgatatcc gtattgatcc
     2341 tattctcaag gtccatggct cttattttta cccggttcag tcaacattgt cgtgcagata
     2401 cgagttcccc gaccggatct ccgtattccg ggtctcggga tcttcgtaca gcgaatattt
     2461 tgaaacatgg gcttcgcccg atgaggtgaa gttcttcagt tccacattcc gcctcacata
     2521 agcgggctca ttctccagct cttcgatcac gctctcctgg tgcatcttca ggctcaggtt
     2581 cctcagccgc tcaatccgct cctgtgaacg cagatcgacc gaaggacgcg taaagacctt
     2641 ttccgtatcc cggaacgtct cgccacccgg ctttttgaca tagacctcga aaggcctgat
     2701 ctcatcctgc ttgccggata cagggcgctc cggctgatcg tcccggccaa tccggttgac
     2761 gatcggctgg tattcgggct tcacaacttc ctgctcttcc cggctgatct cttcgggctg
     2821 ctgaaattca aaaacgaatg tccggtcttc cggctcgggg atattcatct cctgtttttc
     2881 ctccatcacc ggcgcttcga cgctgacggt ctccatttcg gattcttctc cagtcgcttc
     2941 agcggtttcg ggctcgctaa acgattcttc ttcaacggct tcattctctg ccgtctcttc
     3001 catggccggt ttccggagaa gctcaaaggt ccgggcattc atcaggctgt cgtcgatggc
     3061 ctgcatttca ggcatgggcg ttgccgctac tgtctggtta cccagggtgt ggacaacgcg
     3121 gttatcctgg gcatcccctt ttttttgcgg ttcaaatcct gtggcgatca gcgtgatgct
     3181 gatacttttg cccagtttgt cgtcgtagcc gttgccccag atgatctcgg cctgcgattt
     3241 ggcctcctgc tggatgtagt cggtaatttc cgtcacctca tccatcgaaa tttcttcttc
     3301 gcccgaggtg atgtagagca gcacattgct ggcgccttcg atatcattgt cgttgagcag
     3361 gggagaagag agggcgcctt tcaccgcatc cagggcgcgg ttctcgcctt cggcctgggc
     3421 atagcccatg atggctttgc cactctcttt cattaccgtt ttgacatctt caaaatcgac
     3481 gttgatgtag ccggtaacgg tgatgatctc ggcaatgcct ttggctgccg tggtgagcac
     3541 atcgtcggct ttgtcgaaag ccgcagagag cttgaggtcg ccgcaaagct cccggagctt
     3601 gtcgttgcag atgatcacca gggtatcgac atatttcttc aattccagga gaccttcatc
     3661 ggcctgtgtc ctccttttct ttccttcgaa agcaaagggg atggtcacga tgccgacggt
     3721 caggataccg agttcgcggg ccacctgggc aatcaccggt gcggcgcccg tgccggttcc
     3781 gccgcccatg ccggcggtga taaaaagcat ctgggtattt ttctccagga tctcccggat
     3841 ctgttctatt ctgttcagtg cagcttccct gccgaccgca ggtacggagc cggcgcccag
     3901 ccctttttcg cctagctgga tctttgacgg gacgggtgac gattccatgg cctgggcatc
     3961 ggtgttgcag atgataaagt ctacaccttt gattccctgg cggaacatgt ggttcaccgc
     4021 attggacccg ccgcccccta cgcctatgac ttttatgatg gaagactggt tcttcggcat
     4081 atcaaattgg atcatttctt tcatggtcta caggaattta aaaatatttc ttttctgaat
     4141 tggatttgtt ttttatatga ttaattataa acaggttgat tgttgataac tgatgttata
     4201 cctcatctcc ttcaaaccag tcctggatcc gtttcaggaa cccgttggtc ctcggtttct
     4261 tttcaaccgg tttttcttct tcgatctctt cttcctcttg cagttcaggc ttatcatcca
     4321 cagtttcggg gaggggttcc ggtttttgtt cctgcgaagg tgccggggca ggggctgggg
     4381 cgggcaactc ctgttccctg ctctgtggcg ccttatcctg catcttgttc cccgacctga
     4441 cctgttccct tatctgccgt tcgattccct ggataacgag gccgataccg gtggcataca
     4501 tcgggctggc catttcgtcc agcgtctccg gcgccagatg ctcgttcgga tagccgatcc
     4561 gggtgtccat cccggtcatg aattcggtga gctgagccag gtgtttcagc agcgaaccgc
     4621 cgccggtgag cacaatgccg gcaataagtt tcttctcata tcccgaattt ttgatctcat
     4681 aataaacatg ctcgatgatc tcttccatgc gggcctggat aatgctggcc aggttcttca
     4741 gcgtgatctc cttgggctgg cgaccgcgca acccggggat ggcaaccact tcgtcttcgc
     4801 ggttttcgct ggccagcgcc gatccgaacc tgatcttcag ttcttcggca tgttttttaa
     4861 tgatcgaaca accctcttta atatcttccg tcaggatttc accgccgaaa gggataacag
     4921 acgtatgccg gatgatcccg tcctggaaga tggcgatgtc ggtcgtgccg ccgccgatat
     4981 ccaccagcac gacgccggcc tctttttctt cttcgcccag cacggcgtcg gccgatgcga
     5041 tcggctccag gatcaggtcg accacctcca gccccgcttt cctgacacac ttgtaaatgt
     5101 tcttggctgc cgccgtctgg ccgatgatga tgtggaagtt ggcttcgagc gtgttaccca
     5161 gcataccgat cggctgcttg acgtcttctt ccccgtcgac aatatactcc tgcgcgatca
     5221 catcgatgat ctcctccccg ggattcatgt tcaggttgaa catgttctgg tggagggtgt
     5281 cgatgtcctg ctggctgatc tcgttctcga tatcacgcct gatgatggtc cctttgtgct
     5341 gcaggctttt gatgtgctgt ccggcaattc cgacattcac ataccggatc tccaccccgg
     5401 aacttttttc ggccatctcg acagcagtac ggatggactg gaccgtgttt tcgatgttgg
     5461 atacgacgcc tctcttcacc ccgatcgact gggtgttgcc gtatccgaga atttcaattt
     5521 ttccgtgctc gtttcttcgc cctacaatgg cggcgatttt ggtggtgccg atgtctaatc
     5581 cgacgataat atccgactta ttcatagtgt tgggtttttt atacaaacta cctggttctt
     5641 atattttaaa ttgatcgttt tataggtgtt ccaatcgata ttccgcatca ctttccgata
     5701 gaataccttg aggttttcca gcttttcggc ggcatcttcc ccgttaccta acaggatgat
     5761 ctgtgaaccg acccggggta ccagctctat ttccccgttt tcatggatat agatctggtc
     5821 gataaatgat tccaggaatt catcccggga aatggcatat gcaacggggt agatctggtg
     5881 cagaacggag ctcctgtcga acttgtccag cggtttttcc gatttatcga tcgacatcag
     5941 ccgctcggaa atatttccgc cggccagggt gacatgcgac ggatgggccg ggttcagcgg
     6001 catggcacac ccgttcaggt cgaggtagaa ttgctctccc tgccggtttg caatccgcac
     6061 cagcggctcc ctgaccctgg ctttcatgat tagttcgcag tccatcgttt tgtaaacctc
     6121 gcaggcagag acatagggat tgccatcaac cagcttttcc attttatcga tatcgatgat
     6181 cgatacgggc gatcctttga tctcaccaaa gtttttcctg acgagttcat ggatctccga
     6241 tgttgttatg agcgatttat cggtctggtt caggacttcg acggtgaact cacggtacac
     6301 attccggtgg tgctccaggt cggtgaaagc cggcaggatg atgacggcag ccatcaggaa
     6361 gatcaggaac agtatgttca ggagtttctt catgcccgga gtattttttc gatttttccg
     6421 accagaaggt cgatatcgcc tgcgccaatg gtcaggaaga tttcgggcct gttttccgta
     6481 agggattgca gtgcctgttc gcggctcacg attttcttat gcggcagctt cattttttcg
     6541 aggatcattt cggatgatac tcccggaatg ggcaattccc tggcgggata aatgtcaagc
     6601 agccatgctt catcaagcag gtcaaggctt tgcgcaaatc cgtcggccag gtctcgcgtc
     6661 cggctgtaca gatggggctg gaacatgccg gtcagctttt tacccgggta catcatcctg
     6721 acagcggata tgaacgcctc aagttccctg ggatggtggg catagtcgtc gatatacacc
     6781 gggccgggct catccagcct cacgtcgtag cgacgtgtaa cgccggtgaa ggtgctgacc
     6841 gctgccgcaa tctgttccgg cgacacaccc agtttgacgc atacggccgc agcagccatc
     6901 gcgttttcca cgttgtgacg gcccgggatg cggagggcca catcgcgtac catcctgcct
     6961 ttcaggctca tgtcgaaccg ttgtctgccc tcgcggataa ccgggttcag agccgaaata
     7021 tcggcattcc cctccaccga gtaggtgacg gtttcgaaag tgcttttgag atcaacctca
     7081 atcccttcgc gcagcgccag cataccgccc ggtttgatat gagcggcaaa ttcgctgaac
     7141 gattgctgca ggctgcccgt cgatccgtaa atgtcgaggt ggtcggcatc aatggcggag
     7201 atgacggcga tatcgggatg gagatacagg aatgagcggt cgtactcatc ggcttcgacg
     7261 accatgatct cctctttccc ggaggtgacc atgttggaat cgtaattccg ggctattccg
     7321 ccgatcagcg cggtgacggg ccggcctgct tcgatcagga tatgggcaat cagcgagctg
     7381 atggaggtct ttccatgtgt ccccgcgaca gcgacggtgg tccgtccttc cgtcagttct
     7441 ccaacagcca aggcccgttt tatcatgcgg atcccactgg aagcgaggaa tttatattcg
     7501 ttaagtgatt ccggcacagc cggcgtgtat acaata
//
LOCUS       DAOPNH010000167         7469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11170_length_7469_cov_5.886161, whole genome shotgun sequence.
ACCESSION   DAOPNH010000167 DAOPNH010000000
VERSION     DAOPNH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11170_length_7469_cov_5.886161"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PKZ74_09425"
     CDS             complement(<1..1300)
                     /locus_tag="PKZ74_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HPM93019.1"
                     /translation="MKLNYFFVILLIILIGIQSTTSAQIDKNQAIGIVRSNMTNEELQ
                     NYNVLVYPDLIQGQEFNLSPYHVLHSTYDNSWLFFIDMYPQAQWDHNCRYIFVDHQTG
                     NYSTINFKIPPQDYWYGWEYVNDPHPYPAINPIPDSIQAVSYTLNPDPHKYAVFLCWW
                     EGEPCRWNNLSHLYCGIKRNYGFLEENIFVLSGSGVINDTMSEDLDGDLIGPDFDGPC
                     TKDSIEDVFNYLSQVMTEEDLLFVYATTHGHRTGQDTSYLRLWDYDSLFDYELAELAD
                     EIVCSQKIFGIDACHSGDFVDDLDNEHSTIQTCVSGDRLSVISTGFGFHDMSFWWGTA
                     LRGCYPSSDKSKPWENGPYIGEHDSLYTISNIDSVDFNPDDSLGGNSDGFIQFQEAFN
                     FAYRYASDIRDRGGQNYINEGFQGDLLTLNGIEGRVDTSQA"
     gene            complement(1320..2729)
                     /locus_tag="PKZ74_09430"
     CDS             complement(1320..2729)
                     /locus_tag="PKZ74_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93020.1"
                     /translation="MKRRFLIFLIISHIILNFSPCYSQEFEMPGYIYDINTGDIDANG
                     ANDILVSCPYTDTITILYNDGVGNFEIYYYNRPCGFILSGIIDADNLIDIITRDGYYL
                     YCLRNLGNRILSDNIILLPISGTYDVTSIIDINEDSLKDLVFTHTSGEYWGIFKNNGS
                     LTFTNEIIQSGSSTTNPGVGFITDDSLPDIVLTYSAFDRSSVYVNNGNFNFTEVVLEQ
                     TFIGEAFVMNIDNQGTEDFAFLISYSKSIALYKFIGNDQFERQANYYAPGEYNISTFL
                     PADFNMDGYDDFAITRGDWWNGSDSLYIYFNDHNWSFDLNQIFYLGYLGFFNLKSADL
                     NGDLFPDLYMSGAGTNGNKTLKLLWNDGTGNFSWENPVFIKESDSIDCKLNVFPNPFH
                     DNVRIEVQLTNKSRVSVTMYDLFGKIVKEFCDIESHSPQLQINWDGTDEEKGTCPQGI
                     YILTLRSDSDQISKKIVKY"
     gene            complement(3035..3262)
                     /locus_tag="PKZ74_09435"
     CDS             complement(3035..3262)
                     /locus_tag="PKZ74_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HPM93021.1"
                     /translation="MNCVICKNGQTQKGKVIVTLENNGAIILVKDVPAHICDVCEHYF
                     LDEDIAREVLKRGQESLSKGTELEVIRLRVA"
     gene            complement(3264..3548)
                     /locus_tag="PKZ74_09440"
     CDS             complement(3264..3548)
                     /locus_tag="PKZ74_09440"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPM93022.1"
                     /translation="MDCNNLVFSEHIISQMFKRNISVDDVKGILEVGEIIMTYPDDKP
                     YPSYLMLGFLNKRPLHLVVAKNNQAGLCIMVTVYEPDKNSWSLDFKIKIR"
     gene            complement(3777..5030)
                     /locus_tag="PKZ74_09445"
     CDS             complement(3777..5030)
                     /locus_tag="PKZ74_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93023.1"
                     /translation="MNLLIRENIRISVESIRSHMLRTILTITIIAFGIMALIGILTAI
                     DAIKYFLNDNFMMMGANTFSIRNREMMIQIGGRSNDPKRYKRITFDQAMQFKEDFDFP
                     AHVSISAFASHTGTLKYKSEKTNPNVAVLGTDENYIITAGQEIVKGRNFNPNEVYFGA
                     HVVIIGSEVEKDIFKNKEDPVGKIISVGPGKYQVIGVLKEKGSSMGFSGDRQVLLPLT
                     NVDQYFPRPNRSYGIDVSTIDPLQMDQCIGEATGLFRIIRGDRVGEEQTFDIERSDSL
                     AAMLTENIKYVTMAATFIGLITLLGAAIGLMNIMLVSVTERTLEIGIRKAMGATQRTI
                     RNQFLAEAIVIAQMGGILGIIFGIHRQHPVAHHEDQLHHSLALDIARRHALFHRRPGL
                     GNHPRQQSGKTRPDRIAALRVSGEG"
     gene            complement(5027..5962)
                     /locus_tag="PKZ74_09450"
     CDS             complement(5027..5962)
                     /locus_tag="PKZ74_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93024.1"
                     /translation="MIRYIDRKDIDDEKWNACLERAFNGNLYGRSWFLDIVADNWAGL
                     VENDYERIFPLVFRRKFGLSYIYQPFFTQQLGLFSTTRLDAGKLEEFIGAIPSRFRRA
                     EMNLNTLNNPSGTHYKIIPQVNHELDLINSYEKIRQGYSENLVRNLKKAEKAGLSVSR
                     NIKPDDIIALFRQNRGKDIGHLGDTDYMRLKRIAYTCMYKGMAGIQGVYDDRNQLVAG
                     AFFIRSHNKAVFLFSVLSDEGRKSGAMPFLIDAYLRHNAGKHLTFDFDGSNDADLARF
                     YKSFGSREVHYQRVIIDRMPALLKIGVYLYKKIKA"
     gene            complement(5959..7272)
                     /locus_tag="PKZ74_09455"
     CDS             complement(5959..7272)
                     /locus_tag="PKZ74_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM93025.1"
                     /translation="MITVYCDHITPRHHYIFRFIFEDVMGTEFRFISGIDDFLQDKGP
                     KISYSKENPGGGIHFHPYPILSQNGITTQDTETFLWKGLPVFFKVIKPSEWPFDPFSL
                     AFYLVSRYEEYLPFEPDRHGRFRPEESLAFKGGFLGIPLVDMIVKELKSLISSRYPGY
                     AFPVMPFRFVPTIDVDIAFAHRGKGWLRAAGAWIKLLLRADFRQLKERIFVVSGKIPD
                     PYDNFDLHLETAGKSGLSLRYFILIGDFGRYDRNTSYSSSSFKELLKRLSLTADVGIH
                     PSYRSHLRKEIFIKEKKRLEEITGNQVMASRFHFLRVRFSGSFRILIDQGITDDYSLG
                     YSTVNGFRAGTCTPFLFYDIDKEEITKLRIHPFIFMDSAFIDQMKMSPAEATGQIREL
                     VKRVKELGGEAIGIWHNYSLSEKGQYKGWQEVFRTTFKEFQNDNR"
     gene            complement(7253..>7469)
                     /locus_tag="PKZ74_09460"
     CDS             complement(7253..>7469)
                     /locus_tag="PKZ74_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93026.1"
                     /translation="AKKQTINKISFIQVDLDAAYPLILDDIRNNRGRNISRFSYNLFF
                     REMSKSVMQSFGFRTLGDKISHDHGLL"
BASE COUNT         2150 a         1677 c         1533 g         2109 t
ORIGIN      
        1 tcgcctggct tgtatccacc cttccttcga tgccgttgag ggtgaggaga tcgccctgaa
       61 atccttcatt gatataattc tgaccgcctc tgtctcttat atctgaagca tatctgtaag
      121 caaaattaaa agcttcctgg aattgaataa agccatcact attacctccc aatgagtcat
      181 ccggattaaa atcgaccgaa tcaatattgg aaatagtata gagactatca tgttcaccaa
      241 tataaggccc attttcccaa ggtttacttt tatcacttga tgggtaacat cctcttaatg
      301 cagttcccca ccagaagctc atatcatgaa atccaaaacc cgtactaata accgatagac
      361 gatctccaga tacgcaagtt tgtattgtcg aatgctcatt atccaaatca tccacaaaat
      421 ctcctgaatg acaggcatca ataccaaata ttttttgtga acatacaatt tcgtctgcta
      481 gttcagcgag ttcataatca aataaagaat cataatccca caaacgaaga taagatgtat
      541 cctgaccggt tctgtgcccg tgcgttgtgg catatacaaa taaaagatct tcctcagtca
      601 taacctggct taaataatta aagacatctt caatgctatc cttcgtgcaa ggaccgtcaa
      661 aatcagggcc aatgagatct ccatccagat cttcactcat cgtatcatta attacaccag
      721 aacctgataa aacgaaaata ttttcttcca agaatccata attcctcttg attccacaat
      781 aaaggtggct gagattattc catctgcatg gttctccctc ccaccagcag agaaacacgg
      841 catatttatg cggatcagga tttaaggtat aagaaacagc ctggatacta tccggtattg
      901 gattaatcgc aggataagga tggggatcat tcacatattc ccacccatac caataatcct
      961 ggggtggaat cttaaaatta attgtagagt aatttccagt ctgatggtca acgaaaatgt
     1021 acctgcaatt atgatcccat tgggcttgcg gatacatgtc aataaagaaa agccaggaat
     1081 tatcatatgt tgaatgcaat acatgatacg gagaaagatt aaattcctga ccctggatta
     1141 gatcgggata taccaggaca ttgtaatttt gcagctcttc attggtcata tttgacctga
     1201 ctattccaat tgcctgattc ttgtcaatct gagctgatgt tgtggattgt attccaatca
     1261 agatgatcag caggatcaca aaaaaataat ttagtttcat aaggtttggt tttttgtact
     1321 cagtatttta caattttctt tgaaatctga tccgaatctg atcttaaggt cagaatatag
     1381 atgccttgag gacaagttcc cttttcctca tcggttccat cccaattaat ttgaagttgg
     1441 gggctatggg actcaatatc acagaattcc ttcactatct taccaaacag atcgtacatt
     1501 gtaaccgata ctcgtgactt gtttgtaagc tgtacttcaa ttctgacatt atcatgaaaa
     1561 ggattaggga atacatttaa tttacagtca atcgaatcag attctttaat aaagaccgga
     1621 ttttcccatg aaaaattacc ggtcccatca ttccataata gctttaaagt cttatttcca
     1681 ttagtgccgg ctcctgacat atacagatca gggaataaat ccccgtttaa atcagctgat
     1741 tttaaattaa aaaatcccag gtagcctaaa taaaagattt gatttaaatc aaatgaccaa
     1801 ttgtgatcgt taaaatatat gtataatgaa tcggaaccat tccaccaatc acctcttgta
     1861 atggcaaaat catcgtaacc atccatgtta aaatcagcag gcaaaaatgt acttatatta
     1921 tattcacctg gggcataata atttgcttgc ctttcaaact ggtcatttcc aataaattta
     1981 tatagagcaa ttgatttaga ataactaatc aaaaaggcaa aatcctcagt accctggttg
     2041 tcaatattca ttacgaatgc ttctccaatg aaagtttgtt ccaaaaccac ctcagtaaaa
     2101 ttgaaattgc cattgtttac atagactgag gatctgtcga acgccgaata tgtcagcaca
     2161 atatcaggta gagaatcatc cgtgatgaat cctactccag gattagtagt ggagcttcct
     2221 gattgtatta tttcattagt gaaagtcagg ctcccattat ttttaaatat tccccaatat
     2281 tctccagaag tgtgtgtaaa taccagatct tttagggaat cttcattaat atcaattatt
     2341 gaagtaacat cataagttcc tgaaatcggc aataaaatta tattatcact tagtattcga
     2401 ttacctagat ttcttaaaca atacaaataa tacccatcac gggtaattat atcaattaaa
     2461 ttatcggcat caataatgcc acttaatata aaaccacaag gacgattgta atagtaaatc
     2521 tcaaaatttc ccactccatc attatataaa attgttatgg tatcagtata tgggcaggaa
     2581 acaagaatat catttgcgcc atttgcatca atatcacctg tattaatatc ataaatatag
     2641 ccgggcatct caaattcctg ggaataacac gggctaaaat tcaaaatgat gtgggatatg
     2701 attaaaaata ttaggaatct tcttttcatc gtaaaagtct atgaatggtt aaagttaatc
     2761 atattttgtt caatcttata tgaataatga aaagaaaata aattgtctta aaagggaccc
     2821 ctaagatgtt cgcattttac gttttgcgat tttcattttg catggcattt tgcatttttc
     2881 atgatttacc cttaacgcca cacccttcaa tacttcggat tgtttgacga cgcgaaagaa
     2941 tattgtcaaa ctgtgtagaa aatatatatg ataaacctat atgattaaat gtcgattgaa
     3001 gaatcagggg aaactcaagt tttaaagcaa cgtatcaggc tactcttagc ctgataactt
     3061 caagttctgt gccctttgaa agcgattcct gccctctttt taaaacttcc cgggcaatat
     3121 cttcatccaa aaaataatgc tcgcaaacat cacagatatg tgcaggaaca tctttgacca
     3181 ggataatagc accattattt tcgagggtaa cgatgacttt acctttttga gtctgcccgt
     3241 ttttacaaat tacgcagttc attttatctt attttaattt taaaatcgag tgaccaagaa
     3301 ttcttatctg gttcataaac agtaaccatt atacaaagac cagcctggtt atttttagca
     3361 accactaaat gtaacggcct tttattcaga aaaccaagca tcagataact tgggtaaggc
     3421 ttatcatcag gatatgtcat gataatttca ccaacttcaa gaatgccttt tacatcatct
     3481 accgaaatat tgcgtttgaa catttgggag ataatatgtt cactgaatac caggttatta
     3541 caatccatcg agactcaatt caatacaaaa gtaagctaat tctgtggaaa atgaaagcaa
     3601 ttagaaaaac acacaattga tttgacaaat ctatgtctcc gggctgccgg ctgggatata
     3661 cctggttgtt gtcaggaatg aaactggcat actgggttca gctaaattca ttatttcaca
     3721 ttgaacagca ccattcaccg ttcaccgttc acacttcact gctcaccgtt cactcttcac
     3781 ccttcaccgc tcactcgtag cgcagcgatt cgatcgggtc gagttttgcc gctttgttgg
     3841 cggggatgat tcccgaggcc agggcgacga tgaaacaaag cgtgacgccg agcaatatcc
     3901 agagccaggg aatgatgaag ctggtcttca tgatgagcga cagggtgttg ccgatggatg
     3961 ccgaaaatga tcccgaggat acctcccatt tgagcgatta caatggcttc tgccaggaac
     4021 tggttgcgga tggtccgctg cgtagcgccc atcgccttgc ggatgcctat ttcgagggtc
     4081 cgttccgtga ccgataccag catgatgttc attaagccga tagcggcgcc cagcagcgtg
     4141 atcagcccga tgaaggtggc ggccatcgtc acgtatttga tgttttccgt cagcatggcg
     4201 gccagcgagt cgctccgctc gatatcgaag gtctgctcct ctcccacccg gtcgccccgg
     4261 atgatccgga acaggccggt ggcctcaccg atgcactgat ccatctgaag cgggtcaatg
     4321 gtcgaaacat cgatgccgta tgaccggttc ggacggggaa agtactggtc gacattggtc
     4381 agaggaagaa gcacctgccg gtcgcccgaa aagcccatgc tggagccttt ctccttcagc
     4441 acgccgatca cctggtactt gccgggccct accgatatga tctttcccac aggatcttcc
     4501 ttgttcttaa aaatatcctt ctccacctcg ctcccgatga tcaccacgtg ggcgccaaaa
     4561 tagacctcat tcggattgaa attccgcccc ttgacgatct cctgccctgc ggtaatgata
     4621 tagttttcat cggttcccaa aacggctacg ttcggattgg tcttctccga cttgtatttg
     4681 agtgtgccgg tatgagaggc aaaagcggat atggagacgt gggcagggaa atcaaaatcc
     4741 tctttaaact gcatggcctg gtcgaaagtg atccttttat accgcttggg gtcattgctt
     4801 cgacccccga tctggatcat catctcccgg ttgcggatcg aaaaggtgtt ggcccccatc
     4861 atcatgaagt tgtcgttcag gaagtatttg atggcatcga tagccgtcag gatcccgatc
     4921 agcgccatga tcccgaaagc gatgatggta atggtgagga tggtccggag catatggctg
     4981 cggatcgatt cgaccgaaat cctgatgttt tcccggatga ggaggttcat gcctttattt
     5041 ttttgtaaag ataaacacca atttttaata atgccggcat ccggtcgata atcacccgct
     5101 gataatgtac ttccctggaa ccgaagcttt tatagaatcg ggcgaggtca gcgtcgttgg
     5161 aaccgtcgaa atcgaaggtc aggtgctttc cggcgttgtg cctgaggtag gcatcgatca
     5221 ggaaaggcat cgctcccgat ttgcggccct cgtcagaaag gactgagaag aggaaaacag
     5281 ctttgttatg gctccggata aagaaagcgc cggctacgag ctggttgcgg tcgtcgtaaa
     5341 cgccctgtat cccggccatt cccttgtaca tgcaggtata ggcgatccgt ttcagcctca
     5401 tatagtccgt atcgcccagg tggccgatat ccttcccccg gttttgcctg aacaacgcga
     5461 tgatgtcgtc gggcttgata ttcctggaga ccgaaagacc cgctttctcc gcttttttaa
     5521 ggttccggac caggttttcg gaataaccct gccttatctt ttcataactg ttgatcaggt
     5581 cgagttcgtg gttaacctgg ggaatgatct tataatgagt tccggaaggg ttattcagcg
     5641 tattcaggtt catttcggcc cttctgaagc gggaagggat agccccgatg aactcttcga
     5701 gctttccggc atccagcctt gttgtcgaaa atagcccgag ctgctgggta aagaaaggct
     5761 gataaatata ggataaaccg aattttcggc ggaaaacgag gggaaagatc cgttcatagt
     5821 cattttcaac cagcccggcc cagttatccg ccacgatatc caggaaccac gatctgccat
     5881 agaggtttcc gttgaacgcc ctttcaaggc aggcgttcca cttttcatcg tcaatatctt
     5941 ttctgtcgat atacctgatc accgattatc gttctgaaat tccttaaaag tagtcctgaa
     6001 tacttcctgc cagcctttgt attgcccttt ttcggaaagg gaatagttgt gccagatacc
     6061 gatggcctcg ccaccgagtt ctttcacccg ttttacaagt tcccttattt gcccggttgc
     6121 ttccgccggc gacattttca tttggtcaat aaatgccgaa tccataaaaa tgaagggatg
     6181 gatcctaagc tttgtaatct cttctttatc aatatcataa aacaagaagg gggtacaggt
     6241 gcccgccctg aaaccgttca ctgtggaata gcccagcgaa taatcatccg taatcccctg
     6301 atcaattaaa atcctgaaag aaccggagaa cctgaccctg agaaaatgaa agcgggaagc
     6361 catgacctga tttccggtaa tttcctccag tctctttttt tccttgataa atatttcctt
     6421 ccggagatgg gagcggtaag aagggtgaat cccgacatcc gccgtaaggc ttaatctttt
     6481 taataattct ttaaaagatg atgatgaata cgatgtattg cggtcatatc ttccaaaatc
     6541 cccgatgagg atgaaataac gaagactaag gcccgatttt ccggctgttt ccaggtgcaa
     6601 atcaaaatta tcatacggat ccggtatttt ccctgaaacg acgaatatcc tctctttcaa
     6661 ttgccggaaa tcggcccgga gcaaaagttt gatccaggcg cccgcggccc tgagccagcc
     6721 tttcccccga tgggcaaagg cgatatccac atcgatcgtc gggacgaaac ggaaaggcat
     6781 gaccgggaaa gcataaccgg gataacggct gctgatcagc gatttaagct ctttcacgat
     6841 catatcgacc agcggaatac cgaggaaacc gcctttgaaa gcgaggctct cttcgggccg
     6901 gaacctgccg tgccggtcgg gctcaaacgg cagatactct tcgtaccggc tgaccaggta
     6961 aaacgcaagg gaaaacgggt cgaaaggcca ttccgaaggt ttgattactt tgaaaaaaac
     7021 ggggagaccc ttccataaaa aagtctccgt atcctgcgta gtgatcccgt tctgcgacag
     7081 gatgggataa ggatggaaat ggataccgcc gcccgggttt tcttttgaat aacttatttt
     7141 cgggccttta tcctggagaa aatcatcaat accggaaata aaccggaatt ctgtacccat
     7201 aacatcttca aaaataaaac ggaagatgta gtggtgcctg ggggtgatat ggtcacaata
     7261 aaccgtgatc atggctgatt ttatcgccta aagtacgaaa accgaaggat tgcatgacgc
     7321 ttttagacat ctccctgaaa aagaggttat aactgaaccg cgaaatattc cggccccggt
     7381 tgttccggat atcgtcgagg atgaggggat aagctgcatc caggtctacc tgaataaaac
     7441 tgattttatt gatcgtttgc ttcttagcc
//
LOCUS       DAOPNH010000168         7309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11513_length_7309_cov_5.355942, whole genome shotgun sequence.
ACCESSION   DAOPNH010000168 DAOPNH010000000
VERSION     DAOPNH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11513_length_7309_cov_5.355942"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1124
                     /locus_tag="PKZ74_09465"
     CDS             <1..>1124
                     /locus_tag="PKZ74_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93027.1"
                     /translation="ANDALVVKYPNLVGIGTASPDALLHIHKDIYNSSYFGYLDTRAN
                     YFTHREIEADGNGQSCVYASRNRDVRNDGTGYLAIQSNNAIVGSCTWGDLYSFAVFGT
                     NYNDYGRCGGVLGIESDNEGNWGSLGYTSSGLTAYGGYFTTHTSGAGKSDSDLYTSVG
                     MGAWGDLFGADIHGKVYGAYVEGSHYAGYFHGDVIKDKLDVHLQKNATGTNDVLYTSV
                     SADVTVVTSGTAMLSGGKASIAFDPVFSSAVSASESVVVTVTPIGESAGIHLTKVSAS
                     GFSMAENNAGKSDVMVNYIAIGKRAGHENPDPAKEVIAPDYTGKLSRGLHADGNTETD
                     GEGLYFERGELVVGIHPTLLPDPNRSTVEETIIPEQKSPV"
     assembly_gap    1125..1224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1626..2060
                     /locus_tag="PKZ74_09470"
     CDS             1626..2060
                     /locus_tag="PKZ74_09470"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM93028.1"
                     /translation="MKRKATAIWQGNGMEGKGSLNSVSGVFKGTPYSFVTRFKNEVGV
                     EGTNPEELIAAAHGGCFAMALSFALTNAGFVPEELKVEAAVNIEFMTDHFEITGIHLD
                     LAGKVPGIAADKFEELAMAAKAGCPVSRALSAVPITLAVKLA"
     gene            complement(2131..2412)
                     /locus_tag="PKZ74_09475"
     CDS             complement(2131..2412)
                     /locus_tag="PKZ74_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPM93029.1"
                     /translation="MNIFVAKLSSSTTSDDLRTLFEEYGEVTSANVITDRMTGYSKKF
                     GFVEMSNDDEANKAIKELDNCQYDHAEIVVKKARPREDAPREKRYSNYR"
     gene            2677..2907
                     /locus_tag="PKZ74_09480"
     CDS             2677..2907
                     /locus_tag="PKZ74_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93030.1"
                     /translation="MKKDTLENRLNLARLKAKIPDDKLLMGSVIKKYPVVLDGGKTVI
                     YISDKTKVEETRLKYEMLKNSKAPDHYTKKQQ"
     gene            complement(2941..3213)
                     /locus_tag="PKZ74_09485"
     CDS             complement(2941..3213)
                     /locus_tag="PKZ74_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93031.1"
                     /translation="MHRLILIILICHLSTARSRELSICSWNLKDFGSAKNETEIYFIA
                     NTVKGFDVVAIREVVASAVGEEEIIRLYEALETTRKIPVIVRTRMR"
     gene            3422..4585
                     /locus_tag="PKZ74_09490"
     CDS             3422..4585
                     /locus_tag="PKZ74_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93032.1"
                     /translation="MYYSREIEKEVLKSIANNPVTAITGPRQCGKSTLAKHIAELSGK
                     DTLYLDLEKPSDLSKLDDPEWFLGLQKEKLICLDEIQRKPELFPLLRSLVDEWAGNGH
                     FLVLGSASRDLLRQSSESLAGRISYKRLTPFLLSELNNEIQLPDYLSRGGFPRSVLAR
                     DNEQSLRWREDFISTFLERDLLLCSGFSPVTMRKLWQMLAHLNGQVVNFSLLGSSLGV
                     SHTTVRNYVELLQSTFMLHLLQPIASGTGKRLVKSPKVYLSDTGINNALLKIADFEQL
                     AGHPSFGTAWETTVLNNLTGTFPHFNFYFYRTGHGAEIDIVVENGEKRIAVECKASLS
                     PRLSAGTFIAIADVRPLVCLVVTPAEKGWPMKDGVEVVSIGEAIRKIRDLLSM"
     gene            complement(4771..4995)
                     /locus_tag="PKZ74_09495"
     CDS             complement(4771..4995)
                     /locus_tag="PKZ74_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93033.1"
                     /translation="MKNDRLENRMRMQHNKEKISDEKLLMGATCDKFPVVMDNGKTIV
                     YITDKSKEAETRLRYELLKKSRFPMQSDFK"
     gene            5198..5854
                     /locus_tag="PKZ74_09500"
     CDS             5198..5854
                     /locus_tag="PKZ74_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPM93034.1"
                     /translation="MKIAVLGTGMVGEAIGSKLIELGHSVRMGSRTATNEKALAFVAK
                     HPGGKASAGTFAGAAAFGEVIFNCTKGTESMNILRSAGIENLDGKVLVDVANPLDFSN
                     GMPPSLSVCNTDSLGEQIQREFPGVKVVKSLNTMWCGLMVNPGMLNGGDHHVFMSGND
                     AGAKAVVSGLLQSFGWRSENIIDLGDITTARGTEMMLPVWLSLFMAKNHGAFNIRIVG
                     "
     gene            complement(6286..6600)
                     /locus_tag="PKZ74_09505"
     CDS             complement(6286..6600)
                     /locus_tag="PKZ74_09505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPM93035.1"
                     /translation="MDKDIADILEKYLNLVRYRFSGIETAILFGSYAKGKAGSYSDID
                     VALIFSDLKETERFDLQVQLMLIAAQIDSRIEPHPISSAEFKSENHFVHEIQRTGIEI
                     KT"
     gene            complement(6573..6974)
                     /locus_tag="PKZ74_09510"
     CDS             complement(6573..6974)
                     /locus_tag="PKZ74_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPM93036.1"
                     /translation="MTEENRNKLIHYWIDGSDEDFDTMLTLFDSKRYSWALFIGHLML
                     EKLFKAYFVKMNSDYPPYIHNLLRLAELSGLLLTQDQKERLLTITAFKINARYDDYKT
                     SFKLKCTPEFTARWLDELKEIRTWIKTLLIS"
BASE COUNT         2039 a         1513 c         1662 g         1995 t
ORIGIN      
        1 cgcaaatgat gccctggtgg tcaaatatcc taatctggtc ggaataggga cagcatcacc
       61 ggatgcattg ctgcatattc ataaagacat ttacaattca agctatttcg ggtatttgga
      121 tacacgtgca aattatttta cccaccgtga gatcgaagcc gatggcaacg gtcagtcatg
      181 tgtgtatgca agccggaaca gggatgttcg aaatgatggg accggttacc tggctattca
      241 gtcgaataac gctattgtcg gttcctgtac ctggggcgat ttgtattcat tcgctgtttt
      301 cggaacaaac tacaacgatt atggaaggtg cggaggggtc ctgggtattg aatccgataa
      361 tgagggaaac tggggctcat tgggttatac gagcagcgga ttaacggcat atgggggcta
      421 ttttacaacg catacctcag gcgccggcaa aagcgattct gatctttata ccagcgttgg
      481 aatgggcgcc tggggcgacc tcttcggcgc cgatatccac ggaaaagttt atggagccta
      541 cgtggaaggc agccattatg cggggtattt ccatggcgat gtcattaaag ataaactgga
      601 tgtccatctc cagaaaaatg ccactggaac gaatgatgtc ctctatacca gcgtcagtgc
      661 cgatgtaacc gtcgttacca gcggaaccgc gatgctttcg ggcggaaaag ccagcattgc
      721 tttcgacccg gtgttctcat ccgctgtctc cgccagcgaa tctgtggttg ttaccgttac
      781 tcccataggc gaatctgcag gtattcacct cacaaaagta tcagcttcgg gtttctccat
      841 ggctgaaaat aatgccggga agagcgatgt gatggtcaat tatatagcca ttgggaaaag
      901 agccggccat gaaaatcctg atcctgccaa agaagtcatt gcacctgact atacgggtaa
      961 attatcacga ggccttcatg cggatggcaa taccgaaacc gatggcgaag ggctttactt
     1021 tgaaagaggt gaactggttg tcggtattca tccaaccctt ctgcccgatc cgaaccgatc
     1081 aacggtggag gaaacgatca ttccggaaca aaaatccccg gtgannnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnccgaac cgatcaacgg tggaggaaac gatcattccg
     1261 gaacaaaaat ccccggtgag aaatggtgtt gctgatgacg gaagacatgc tggcgacgat
     1321 ccagaatgat tttaatggta tctttgagga ttagtatctg agctataatc tgcatctgat
     1381 tttgacagga gtaaggctgt atcaatagaa ttaaaccaca aaggacacca aggatttcac
     1441 aaagcacaca aaggtactgg ccagataatc aattatattg tgcccttcgt gaatttcttt
     1501 gtgccctttg tggttaaata taagactatt gatgcaccca cctgcatcta ctttaacagc
     1561 ttgtaacctg tagcatcagt acttaaaaat atcgatactg acattaatac tttaaacgat
     1621 tgattatgaa aagaaaagca acagccatat ggcagggaaa cgggatggaa ggcaaaggga
     1681 gcctgaactc cgtcagcggg gtatttaaag gcactccgta ttcatttgta acgaggttta
     1741 agaacgaagt gggagtggaa gggaccaacc ccgaggagct gatcgcggcg gcgcacggcg
     1801 gatgtttcgc catggcgctg agctttgcgc tgaccaatgc cggattcgtc cctgaagagc
     1861 tcaaagtgga agcagcggtt aatatcgaat tcatgaccga tcatttcgag atcacgggta
     1921 tccacctcga tctggcggga aaagtccccg ggatcgcggc ggataaattc gaagaactgg
     1981 ccatggcggc caaggctgga tgcccggtgt cgcgggcgtt gagcgcggtg cctatcacat
     2041 tagcggtgaa actggcttaa aaaaaagagt gccccggctt ccgggacact ctttttgctt
     2101 taacttgaag cagtaagtgc cactgatatc ttaacgatag tttgaataac gtttttccct
     2161 tggagcatct tccctgggcc ttgctttttt gacgacgatt tcggcgtggt catactggca
     2221 attgtcaagt tccttgatcg ctttgttcgc ctcgtcatcg ttcgacatct caacgaatcc
     2281 gaatttcttc gaataacctg tcatacggtc ggttatcaca ttggctgaag tcacttcgcc
     2341 atactcttca aataaagtcc ttaagtcatc gcttgtcgtg gatgagctta gttttgcaac
     2401 aaaaatgttc atgattagat aaaataaaat tgataaatac gttaggtcga aaactgtaag
     2461 aaaggcagat cgtcggtagg aaatgcgaat caatataatt ttaaacgcgg caaaggtaaa
     2521 taaataattg attctcaaaa cattttattg ggatttggcg ttaataaaat aatcaactta
     2581 aaaaataaaa taatataaat atatgtaaaa attaaatatt tgtcgtatct tgtacgaaat
     2641 attgattcac acttttatca aattcacata catacaatga aaaaagacac gttggaaaac
     2701 aggttgaatt tagcgcgcct gaaagcgaaa atccccgatg acaaactttt gatgggctcc
     2761 gtaataaaaa aatatcctgt agtccttgat ggtggaaaaa cagttattta catatccgat
     2821 aaaaccaagg ttgaagaaac caggctgaaa tatgagatgc tgaagaacag caaggcaccg
     2881 gaccattaca caaaaaaaca acaatagttt tctgcattgc cggatggcgg cgatgatcct
     2941 tcatctcatc cgggttctca caatgaccgg gatcttacgg gtagtttcaa gggcctcata
     3001 cagccggatg atttcctctt cgccgacggc agaagccacc acttcccgga tagcgaccac
     3061 atcaaacccc ttaactgtat tggcaatgaa atagatctcc gtttcatttt ttgcacttcc
     3121 gaaatccttc aggttccagg agcagatgga gagttcccgc gaccgggcgg ttgagaggtg
     3181 gcaaataaga atgattagga tgaggcggtg catgtcctga ttttgtagcc aggcgaaggt
     3241 aaaacaaaaa ctgatcaccg ttgcatcgga tgctgacggc cggatgcaaa tgcaagctgc
     3301 cggtaaagac aaacgcaggt cagtttgcac gggtatgcca gtgcctccaa ctataaaata
     3361 tgaaaataat acgtgcaata tgaaaattat actttaaatt tgcacggtaa tagaatcatc
     3421 catgtattat tcccgggaaa tagaaaaaga ggtcttaaaa agcattgcta ataatcccgt
     3481 tactgcaatt acagggccca ggcaatgcgg aaaatcaacg ctggcaaagc atattgcgga
     3541 gctttccggg aaggatactc tttaccttga ccttgagaag ccatctgatc tcagtaaact
     3601 ggatgatccg gaatggtttt taggattgca gaaagaaaaa ttaatttgcc tggatgaaat
     3661 acagagaaag ccggagctgt tcccattact gcgttcattg gttgatgaat gggccgggaa
     3721 tggacatttt ctggtattgg gctctgcctc acgggatctg ctccggcaga gctcggaaag
     3781 ccttgcggga aggatctctt ataaaagatt gacgccattt ctgttaagtg agttgaataa
     3841 cgaaatccaa ttaccggatt acctgtcaag gggaggtttc cccagaagcg ttttagccag
     3901 ggataatgaa caatcattga gatggaggga ggattttatc tccactttcc tggaaaggga
     3961 tcttctgtta tgttcgggct tttcaccggt taccatgcgt aaattatggc aaatgcttgc
     4021 ccacctgaat ggccaggttg taaatttttc actgctgggt agttcgcttg gggtaagtca
     4081 tacaaccgta aggaattacg tggagctgct gcaatcgaca ttcatgcttc atttacttca
     4141 acctattgca tctggcacag gcaaacggct ggtaaagtcg cccaaagttt atctcagcga
     4201 cacagggata aataatgcct tgcttaagat tgccgatttt gagcaactgg ccggccaccc
     4261 ttctttcgga acggcttggg aaaccactgt tcttaacaat ctgacaggga catttcctca
     4321 tttcaatttt tatttctaca ggaccggcca tggggctgaa attgatattg ttgttgaaaa
     4381 cggtgaaaaa agaattgctg tcgagtgcaa ggcaagtttg tcgcccagac ttagtgcagg
     4441 tacattcatc gctattgcag atgtccggcc gctggtatgt cttgttgtca ctccggcaga
     4501 gaaaggctgg ccgatgaagg atggagttga agtggtcagt atcggtgaag cgatccggaa
     4561 gatcagggat ctgctcagta tgtgaccatt atattccccc ttcggcataa ctcattcgta
     4621 gcagctttgc tgttgcaaat ctgcaaagaa tgagtataaa acagctgcgg ttgatctcct
     4681 tgttcgcctc atcttcattc gacatttcaa cgaatctgaa tttattcgaa tgacctgtca
     4741 tgcggttggt gatcgcattg gctgaagcgt tcatttaaaa tcggactgca tcgggaacct
     4801 gctcttcttc agtaattcat accgtagtct cgtttcggct tccttgcttt tatcggtgat
     4861 atatacaatc gttttgccat tatccatgac tacagggaat ttatcacatg ttgcccccat
     4921 cagtaatttt tcatcggaga tcttttcttt attgtgttgc attctcatcc tgttttccag
     4981 ccggtcgttt ttcatcttga tatttataag aatatatgtg atttaaaata tttcacaaga
     5041 tacgaataat atatattaca ttatcatatt tactgaagtt tatttattaa caatacttta
     5101 aatcacggtc aaatggaatg aaatataact taacgttgta attgtggcct gctgaacctt
     5161 ttccggtggc ggttgtttat ctgtgaaact ttaactcatg aaaatagccg tactgggaac
     5221 cgggatggtc ggagaggcca tcggttcaaa attaatagag ctcggccatt cggtcaggat
     5281 gggctcgaga acagcgacca acgagaaagc attggccttt gtggcaaagc atccgggagg
     5341 caaagcttcg gcggggactt ttgccggcgc agccgctttt ggcgaggtca tcttcaactg
     5401 cacgaaaggg acggagtcaa tgaatatcct ccgcagtgcc ggcatagaga acctcgacgg
     5461 caaagtgctg gtcgatgtag ccaatccgct cgatttctcc aacggcatgc cgccatcgtt
     5521 atcggtttgc aacaccgatt cgctcgggga gcagatccag cgggaatttc ccggggtgaa
     5581 agtcgtaaag agcctcaaca ctatgtggtg cggactgatg gtcaatcccg gcatgctgaa
     5641 cggaggcgac caccatgtgt tcatgagcgg caacgacgcc ggggctaagg ccgttgtatc
     5701 cggcctgctg cagagtttcg gctggaggag cgagaacatc atcgacctgg gcgacatcac
     5761 cactgcccgc ggcaccgaaa tgatgcttcc tgtatggcta agcctcttta tggccaaaaa
     5821 ccatggcgcc ttcaacatca ggattgtggg gtagatcggg aatgaggggt gggatgtgct
     5881 gaattcgaac cggtgacctc atttcttaca ggattaatct tattatttcg gacaaacaag
     5941 gccacctatt tcggtgtaaa gtaggccagt catatactac aacctctttg ccccgacatt
     6001 tttaattttt caccctttcc atgccctgac attttcattt tatacatctt tttttgtgag
     6061 tttccccgaa cctcaaccat ataaaaaaag agtaaaaaat acccattgtg aatgataatg
     6121 tttattagtg tgttgatggc gaaatggaag agaagatatt cgattgtcga tggtcgattg
     6181 tcgatggtcg atgggaaata agcgggaatc tgcgtctaaa tccgcgaaaa tctgcgagaa
     6241 aataaagtaa aggatgaatt tcttttactt gaaaataatg tcgggtcagg ttttaatttc
     6301 aatccctgtt ctttggattt catggacaaa gtgattttca gatttaaatt cagcagatga
     6361 aatcgggtga ggttcaattc gggaatcaat ttgtgctgca atcagcatta actgtacctg
     6421 taaatcaaat cgttctgttt cttttaaatc actgaagatg agagcgacat ctatgtcact
     6481 ataactcccg gccttccctt tggcataaga accaaacaag atagctgttt ctataccgct
     6541 gaatctatat cgaacaagat ttaaatactt ttctaagata tcagcaatgt ctttatccat
     6601 gttcttattt cttttaattc atccagccac cttgctgtaa attccggagt gcatttcagc
     6661 ttaaaactgg tcttgtaatc atcgtatctg gcattgattt taaatgcagt tatcgtaagt
     6721 agtctttctt tctggtcttg ggtcaataat aatcctgaaa gctccgccaa tcttaacaga
     6781 ttgtgaatat aaggaggata atctgaattc attttcacaa aatatgcctt aaaaagtttt
     6841 tcaagcatta aatgtccgat aaatagtgcc caactatatc gctttgaatc gaacaaggtc
     6901 agcatcgtat caaaatcttc gtccgaacca tcaatccaat agtgaataag tttattccgg
     6961 ttttcttctg tcattttcat tacttcaaag atacaaaaaa tccaataagt aattaaccat
     7021 aatcatatca acccgttgac attgacaatt caacttaaaa agatgctgac aactggaatg
     7081 caattcacgc aaatcaaagc gctgctttcg cagaaattta ttgtaatgat tattatcagc
     7141 gggaatctgc gtctaaatcc gcgaaaatct gcgagaaatt atgttccggt aataacatct
     7201 tttttataac tttgcacccc taacgggatg ttagctcagt tggttcagtc ccgagcactc
     7261 gggatcacac gcatgagttc tttatgggat gttagctcag ttggttcag
//
LOCUS       DAOPNH010000169         7302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11533_length_7302_cov_5.840210, whole genome shotgun sequence.
ACCESSION   DAOPNH010000169 DAOPNH010000000
VERSION     DAOPNH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_7302_cov_5.840210"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKZ74_09515"
     CDS             <1..297
                     /locus_tag="PKZ74_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPM93037.1"
                     /translation="SIYVLLVAVFFKLPFTLSFNALLGIGYIGIFEMGVAYVLWLKGL
                     QLSETTAKVSNLLFIAPFFSLIFINIFVGERILWSTLAGLAFVVTGIIMQRHIK"
     gene            340..1608
                     /locus_tag="PKZ74_09520"
     CDS             340..1608
                     /locus_tag="PKZ74_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPM93038.1"
                     /translation="MKTLKYLLLLILFLSIGRQVSAAYLLIPMDDTQRNHLKAYGIAY
                     WVLTKDVEIYWLLNYRGGSYMFPHNKSFEDECIIRGVSYELIADVQSTAILEEIADPQ
                     VNMDAVKLHKAPKIAVYSPKNKLPWDDAVTLVLSYSEIPYDVIYDKEVIEGILPMYDW
                     LHLHHEDFTGQYGKFWSAYRSFPWYQEDVRINEETARALGFQKVSECKLAVAQKIRDY
                     VIGGGYLFAMCSAPDSYDVALSAEGVDICDIVFDGDPMDPDAQAKIHYDNCLAFTDFT
                     LSTNPYEYEISSIDVTETRPKNIEGKDFFTLFDFSAKWDPIPTMLCQNHTQVIKAFMG
                     QTTAFNKDQVKSNVLVMGENKPLNEARYIHGEFGKGFWTFYGGHDPEDYSHRVGDPPT
                     ELDLFPNSPGYRLILNNILFPAARQQKRKT"
     gene            complement(1609..2463)
                     /locus_tag="PKZ74_09525"
     CDS             complement(1609..2463)
                     /locus_tag="PKZ74_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93039.1"
                     /translation="MKMHIKSRKTFFLLLLLAASCVLLLHACDETGDEIDYQPNVNSS
                     KDFVRAEDALYDIVNAFLKGIYDSLIAQNNYAYIDYCGVTYDPDENIMHFSYGAVNRM
                     CQDGKFRRGDFFAEFDGPLFEPGVKANLITDSLFVNDSLVFASMTFNNLGLNAMEKVQ
                     YGLNVISLSVAPRDSLSLRRIQITADLNLSWDQGSSTPEIHEDDYYLVTGTASGISSD
                     FTDFSVMIEEPLVNYVDCCWIISGTNLITVPSADIKQGMIDYLSDDGCNYEVHFYFNE
                     NFFYDHMN"
     gene            complement(2478..2969)
                     /locus_tag="PKZ74_09530"
     CDS             complement(2478..2969)
                     /locus_tag="PKZ74_09530"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPM93040.1"
                     /translation="MKNISIIVAIARNYAIGRNNQLLWHLSEDLKRFKKITLGKQVIM
                     GKLTYLSLPVRPLPNRQNVVITDRKDEVFEGCTTVHSIEEALDLCHEAEESFIIGGGS
                     VYRQFLPYCNRLYITLVHKDFDADTFFPQFDLAQWKLVEREDRLSDNDSELSYSFLIF
                     EKA"
     gene            complement(2966..3760)
                     /locus_tag="PKZ74_09535"
     CDS             complement(2966..3760)
                     /locus_tag="PKZ74_09535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPM93041.1"
                     /translation="MIQYLELLDHVRKNGVEKADRTGTGTLSVFGYQMRFDLQKGFPV
                     LTTKKLHLRSIIHELLWFLKGETNLKYLHENKVTIWDEWAGPNGDLGPVYGYQWRSWP
                     AGNGKHIDQISNVIHSIKTNPDSRRHIVSAWNVGELDKMALPPCHLLFQFYVAEGKLS
                     CQLYQRSCDIFLGVPFNIASYALLTLMVARVTGLQPGDFVHTLGDAHIYLNHIEQVML
                     QLSRKPYPLPKMNLNPERTNIFDFVFEDFELVDYQCHPHIKGEISV"
     gene            complement(3812..5230)
                     /locus_tag="PKZ74_09540"
     CDS             complement(3812..5230)
                     /locus_tag="PKZ74_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPM93042.1"
                     /translation="MKLKSLLLIFVMMIISSMPLESTAQTGQDVTQALSEAGAGFSIQ
                     SLLRGILGMMVLLGICYLFSTNRKAISWRVVIFGLLLQAVLAITVLKVPAIQHVIEFI
                     ARVFIKILDSSNAGSSFLFKSLVTNKVETGLITFAFQILPTIIFFSALTSVLFYYGII
                     QKVVWAMAWLLTKLLKISGAEGLVASSNVFLGQTEAPFLIKEYLPKMNRSEIMLVMVA
                     GMATMAGGVLAIYIGLLGGDDPVGKMIFAQHLITASVMAAPAAVVTSKMLVPQTEPIE
                     SDIKITKERIGSNILDAIANGTTSGLKLAVNVAAMLIAFIALIYFLNFIFGKIGDWTH
                     VNAWVADITGGQYSKISLQFFLGYLLAPLTWLMGVNSHDMTLVGQLLGEKIILNEMIG
                     YASLQNLISSGVFYDQKSVIIAVYVLCGFANFSSVGIQIGGISAIAPNQRKTLSDLGF
                     RALLGGGLSALLSATLVAIILG"
     gene            complement(5290..5874)
                     /locus_tag="PKZ74_09545"
     CDS             complement(5290..5874)
                     /locus_tag="PKZ74_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPM93043.1"
                     /translation="MVRIRLEIVGMSYSQSQSGAYALILGEVKGNRRLPIIIGGAEAQ
                     AIAVELEKIKPTRPLTHDLFKNFADTYHIQVKEVIIDQFRQGVFHAKLVCMQNEKESL
                     IDSRTSDAVALAIRFKCPIYTYEKIMAEAGMIMDENANLSDTMTTPLEESSESSFKDL
                     NLAELEELLQKAVDREDYEKASQIRDEIKKRKKG"
     gene            6018..>7302
                     /locus_tag="PKZ74_09550"
     CDS             6018..>7302
                     /locus_tag="PKZ74_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPM93044.1"
                     /translation="MKKLISLLSLFLLPLLTFASEANLVIPDAVKDQSILYWGFLITV
                     LGLLFGLYQFVQVKKLKAHHSMLEVAQVIYETCKTYLLQQGKFLMILFVFIGATVAFY
                     FGYLSEGGFGIGAVLMILGWTVVGILGSYSVAWFGIRMNTLANSRMAFASLEKKPIKL
                     LNIPLAAGMSIGVVLICVELIMMLIILMFVPGEYAGASFIGFAIGESLGASALRIAGG
                     IFTKIADIGSDLMKVVFKIGEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTGVA
                     LISFILLAVHDTATQTQLLTWIFVMRILMILTSIFAYWINRAISQTRLGKKDDIDFEQ
                     PLTSLVWITSILSILVTFVASYLMIGQLENNLWITLSIIISFGTLGAALIPEFTKIFT
                     SPKSRHVNEIVEASREGGASLTILSGLVAGNFSAFW"
BASE COUNT         1928 a         1713 c         1660 g         2001 t
ORIGIN      
        1 tctatctatg tcctgctggt tgccgtcttt ttcaaattgc cgttcaccct gtcattcaat
       61 gctctgctgg gcattggtta tatcgggatt ttcgaaatgg gtgttgctta tgttttatgg
      121 ctgaaagggc ttcaactctc tgaaacaaca gccaaagtca gcaacctttt atttatcgct
      181 ccttttttct ccctgatttt tatcaatatt tttgtaggtg aaaggattct ctggtctacc
      241 cttgccggtc tggcatttgt ggtcacggga atcatcatgc agcggcacat caagtgattg
      301 tcctgtaaaa caaaaatctt ttgtatccgt ttgttagtta tgaaaacatt aaaatacctg
      361 ttgcttctta tcctttttct ttcgatcggc cggcaggttt ctgctgccta cttgctgatc
      421 ccgatggacg acacccagcg caaccatttg aaagcctacg gcattgccta ctgggtactg
      481 accaaagatg tcgagatata ctggctgctt aattaccgcg ggggcagtta tatgtttccg
      541 cacaataaat cctttgagga tgaatgcatt atccgcggcg tcagctatga actgatcgcc
      601 gatgttcaaa gcacagccat tcttgaggaa atcgccgatc cacaggtcaa catggatgcg
      661 gtaaagcttc acaaggctcc taaaatcgcc gtgtattcgc caaagaacaa gcttccgtgg
      721 gatgatgccg taacgctggt gttatcctat tcggagatcc catacgatgt gatctatgac
      781 aaagaagtga ttgaaggcat tttgccgatg tacgactggc tgcatcttca ccacgaagat
      841 ttcacggggc agtatggcaa attctggtcg gcataccgca gtttcccctg gtaccaggaa
      901 gatgtaagaa taaatgaaga gacggcccgt gctctgggtt tccagaaggt ttccgagtgc
      961 aaactggcgg tagcgcaaaa aataagggat tacgttatcg gaggcggtta cctgtttgcc
     1021 atgtgctcgg cccccgacag ttatgatgtg gccttatccg ccgaaggcgt tgacatttgc
     1081 gacatagttt ttgacggtga tccgatggac ccggatgccc aggcaaaaat ccattacgat
     1141 aactgtcttg cttttaccga tttcacattg agtacgaatc cgtacgagta tgaaatttct
     1201 tcaattgatg tgacagagac ccggcccaaa aatattgaag gaaaagactt tttcacctta
     1261 tttgattttt ccgccaaatg ggatccgatt ccaaccatgt tgtgtcagaa ccatacacag
     1321 gtcatcaaag cattcatggg ccagacaacg gcattcaaca aagaccaggt taaatcgaac
     1381 gttctggtta tgggtgaaaa caaaccgtta aacgaggccc gatatattca cggggagttc
     1441 ggcaaaggat tctggacatt ttatggaggg cacgatccgg aagattactc tcatcgtgtt
     1501 ggcgatccac cgacagagct ggacttattc cccaattctc cgggttacag gctgatcctc
     1561 aataatatcc ttttcccggc agcccgccag cagaaaagaa agacctgatc aattcatatg
     1621 atcatagaaa aaattctcat tgaagtaaaa gtgtacttca taattacacc catcgtccga
     1681 taaataatct atcatacctt gtttaatatc ggccgaaggg actgtaataa ggttggtccc
     1741 tgaaataatc cagcagcaat ccacgtaatt taccagcggt tcttcgatca tgacactaaa
     1801 gtcggtaaaa tcggaggaga taccggaagc agtacctgtt accagatagt aatcgtcttc
     1861 atgtatttcc ggtgttgaag acccctgatc ccagctcagg ttcaggtctg ccgtaatctg
     1921 gatccgtcgc aggcttaaag aatctcttgg ggctaccgat aatgaaatta cattgaggcc
     1981 gtattgaact ttctccattg catttaatcc cagattatta aaggtcatac ttgcaaagac
     2041 caggctgtcg ttcacaaaca aactgtcggt aatcaaattc gccttaacac ccggttcgaa
     2101 caaagggccg tcaaattcag caaaaaaatc gcctctcctg aattttccat cctggcacat
     2161 acgattaaca gcaccataac taaagtgcat aatattctca tccgggtcat acgtcactcc
     2221 gcagtaatcg atataggcgt aattattttg tgcgatcagg gagtcgtaaa tacctttcag
     2281 aaatgcattg acgatatcat aaagggcatc ttcagccctg acaaaatctt ttgagctgtt
     2341 cacattaggc tgataatcaa tttcatcacc tgtttcatca caagcatgca acaaaagaac
     2401 acatgatgcg gctaataaga gcagcagaaa gaatgttttc cgtgatttaa tatgcatttt
     2461 cattaatatt ccttcgttta agctttctca aaaataagga aagaataaga taattccgaa
     2521 tcattatccg aaagcctgtc ctctctttca accaacttcc attgtgccag gtcgaactgc
     2581 ggaaagaaag tatcagcatc aaaatcttta tggacgaggg ttatataaag cctgttgcaa
     2641 tagggcaaga actggcggta gaccgatcct ccgccaatga taaagctctc ttccgcttcg
     2701 tggcaaagat ccagcgcttc ctcaatacta tgaacggttg tacaaccttc aaaaacttca
     2761 tcttttctgt cggtgataac aacattttga cggtttggca ggggccttac cggtaagctc
     2821 agatacgtca gcttacccat gatcacctgt ttgcccaggg ttattttttt aaaccgcttc
     2881 aggtcttctg agaggtgcca gaggagctgg ttattgcgtc caatggcgta attccgggcg
     2941 atagccacaa tgattgagat attcttcata ccgagatttc tcctttaata tgaggatggc
     3001 attggtaatc taccaattcg aaatcctcaa aaacaaaatc aaaaatattg gtccgttccg
     3061 ggttcagatt cattttgggc aacgggtaag gctttcttga caactggagc ataacctgtt
     3121 ctatatgatt gagatagata tgggcatctc ccaaggtgtg cacaaaatct cccggttgca
     3181 ggcccgttac acgggcaacc attaaagtca gcagtgcgta agatgcgata ttgaaaggca
     3241 cgccaaggaa aatatcgcag ctacgctggt agagctggca cgacaatttc ccttcagcca
     3301 cgtagaactg aaaaagaagg tggcagggag gaagtgccat tttatccaat tcgccgacgt
     3361 tccaggcgct tacaatgtgc ctgcggctat cgggattcgt tttgatggaa tgaattacgt
     3421 tgctgatctg gtcgatatgt tttccatttc cggcaggcca tgaccgccac tggtagccgt
     3481 aaacagggcc caggtcaccg tttggaccgg cccactcatc ccagatggtc accttattct
     3541 catgcaggta cttcaggttt gtttctcctt tgagaaacca gagcaattca tggatgatcg
     3601 accgaaggtg caattttttt gttgtcagta ccgggaatcc cttttgcagg tcgaaccgca
     3661 tctggtagcc aaaaacgctg agggtaccgg taccggtcct gtcagctttt tcaacgccgt
     3721 ttttccggac atggtcgagg agttcgaggt attggatcat gtttaaggtg ccctggctaa
     3781 aatttcagtt aataacgacg attactgcgt ctcatcccag gatgatggcc accagggtgg
     3841 cggataagag tgccgagagc ccgcctccga gtaaagccct gaatcccagg tctgataaag
     3901 ttttgcgctg gttcggagcg atggcgctga tcccgccgat ctgaattccg acggatgaga
     3961 aatttgcgaa cccacataac acgtaaacgg cgatgatcac cgatttctga tcatagaaaa
     4021 caccgctgga gatgaggttc tgcaggctgg catagccaat catttcattc aggatgattt
     4081 tttctccgag aagttgcccg acgagcgtca tatcgtgcga attgacgccc attagccagg
     4141 tcagcggggc cagcaggtaa ccgagaaaga actgcagact gattttcgaa tattgtcctc
     4201 cggtaatgtc ggctacccat gcattcacgt gggtccagtc gccgattttg ccaaagataa
     4261 agttcaaaaa atagataagt gcgataaaag caatcagcat agcggcaaca ttaacagcca
     4321 gttttagccc cgaggttgta ccgttggcga tggcatccag gatattgctg ccaatccttt
     4381 ccttggtgat cttgatatcg ctttcgatcg gttcggtttg cggcacgagc atttttgaag
     4441 tcaccaccgc ggctggtgca gccataacgg aagctgtgat cagatgctgg gcaaagatca
     4501 ttttccctac cgggtcatca ccgcccagca aaccgatata gatggccagt acaccgccgg
     4561 ccatcgtcgc catcccggcc accatcacca gcatgatctc cgaccggttc attttcggca
     4621 ggtattcctt gatgaggaaa ggggcttcgg tttgtccgag aaaaacattg ctcgaagcga
     4681 caagcccttc tgcgcctgaa atctttaaga gcttggtcag cagccaggcc atggcccaaa
     4741 cgaccttttg aatgatacca taataaaaca ggacgcttgt gagggcagaa aagaagatga
     4801 tcgttggcag gatctggaat gcgaaagtga tcagcccggt ttccacctta ttagtcacca
     4861 gtgatttaaa aagaaatgaa ctgccggcat tggatgaatc gagtatcttg atgaaaactc
     4921 tggcgataaa ttcaataaca tgttgtattg cagggacttt aagaacagta attgccagga
     4981 cagcctgcaa aagcaacccg aaaatgacca cccgccagct gatggctttg cggttcgtgc
     5041 tgaaaagata gcatatacca agcagcacca tcattcccaa aatccccctg aggagtgact
     5101 gtattgaaaa tcccgccccg gcttcgctca gtgcctgtgt tacatcctgc ccggtttggg
     5161 ccgtggattc taaaggcatg ctgctaatga tcatcatgac aaagatcaac agtaatgatt
     5221 tcagtttcat caaaatagtt cttattaaag gtctaatact ggaacagctt tattcaaaaa
     5281 gaaaaaaact catcccttct ttcttttttt tatttcatcc ctgatctggg aggctttttc
     5341 ataatcttcc cggtcaacag ctttctgcag gagttcttcc agttcggcaa ggtttaaatc
     5401 cttaaacgaa ctttcactgg attcttccag gggagtggtc atggtatcgg ataaattggc
     5461 attttcatcc atgatcattc ccgcttcagc cataattttc tcgtaggtat aaatcgggca
     5521 tttaaagcga atagccaaag cgactgcatc ggaggtcctg gaatcgatga gactctcttt
     5581 ttcattctgc atgcagacca gttttgcatg gaaaacaccc tggcggaatt ggtcgatgat
     5641 cacctctttc acctggatat gataagtatc ggcaaaattt ttaaagaggt catgggtcaa
     5701 aggccgggta ggtttaatct tttccagctc aaccgcaata gcctgagcct cggcccctcc
     5761 aatgataata ggtagccggc ggtttccttt aacctcacca aggataaggg cataagcccc
     5821 gctttgcgac tgactgtaag acatcccgac gatctcaagc ctgatcctga ccataaaaat
     5881 tcagttaacc cttttaccgg ataaaaatag aataaatttc aagccgcata tcccctgttg
     5941 ataaaataga attgcgattt tgaaatactt ttttaatttt gttttcctca ctcatacaaa
     6001 accaaaaaat aaccattatg aaaaaattaa tctctttact ttccctattt ctattgccat
     6061 tgctgacatt tgccagcgag gccaatctgg taattcccga tgcggttaag gaccaatcca
     6121 ttttatattg ggggttcctg attacggtac tgggtttact ctttggcctt taccagtttg
     6181 ttcaggtgaa gaaactgaaa gcccatcatt cgatgctgga agtggcacag gtgatttatg
     6241 aaacctgcaa aacatactta ttgcaacaag gtaagttcct gatgatcctg ttcgtcttta
     6301 tcggagctac ggtggccttt tatttcggtt acctgtcgga aggcggattc ggaatcggcg
     6361 ctgtgctgat gatcctgggc tggaccgtag ttggaatcct ggggtcttac tcggtcgcct
     6421 ggtttggtat caggatgaac acactggcca actccaggat ggcctttgcc tcgctggaga
     6481 agaaacccat caaattattg aatattcccc tggcggctgg gatgagcatc ggcgttgtgc
     6541 tgatctgtgt cgagctcatt atgatgctca tcatcctgat gtttgttccc ggtgaatatg
     6601 ccggggccag cttcatcggt tttgctatcg gagaatccct tggagcctct gcactaagga
     6661 ttgccggcgg tattttcaca aaaattgccg atatcggatc tgacctgatg aaagtcgtgt
     6721 ttaagatcgg tgaagatgac ccgagaaatc cgggcgttat tgccgactgt accggtgaca
     6781 acgccggcga ttcggttggc ccgacagcgg acggttttga aacctatgga gtgaccggcg
     6841 tcgcactcat ctcctttatt cttctggcgg ttcatgatac ggcaacgcaa acccagcttc
     6901 tgacctggat atttgtcatg cgtatcctga tgatcctgac ctccattttt gcttactgga
     6961 tcaatcgggc cattagccaa acccggcttg gaaagaagga tgacattgat tttgagcagc
     7021 ccctcacctc cctggtttgg atcacctcta tattatccat tttggtgacc tttgtagcca
     7081 gttacctgat gatcgggcag cttgaaaaca atttatggat aaccctgtcg atcattatca
     7141 gcttcggtac cctgggcgcg gcgcttattc ccgagtttac gaaaatattc accagcccca
     7201 aatcaaggca cgtcaatgaa attgtagagg cttccaggga aggcggcgca tcgctaacca
     7261 tcctctccgg cttggtagcg ggtaatttca gcgcgttctg gc
//
LOCUS       DAOPNH010000170         7259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11634_length_7259_cov_7.299139, whole genome shotgun sequence.
ACCESSION   DAOPNH010000170 DAOPNH010000000
VERSION     DAOPNH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11634_length_7259_cov_7.299139"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKZ74_09555"
     CDS             complement(<1..267)
                     /locus_tag="PKZ74_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM93045.1"
                     /translation="MRPIRMVDLKTQYDKIKPEVDAAIHEVIDTTSFINGPAVQRFQK
                     NLEQYLGIKHVIPCANGTDALQVSMMALDLKPGDEVITVSFTFVA"
     gene            382..1143
                     /locus_tag="PKZ74_09560"
     CDS             382..1143
                     /locus_tag="PKZ74_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93046.1"
                     /translation="MRFVSIAVLVIITQGLLAQVEVNEEDSALSIPMFYGFYGYQWPG
                     GDLDEQFGSNSVVGPGFLWKTKSNWLFGAEYDFHFGNNVKNTKEIFKNIYTTNGNVIS
                     GDGVPALVATFERCNSFSARFGKIIPVSENDRNSGIFFTVGAGYLSHKIKIEVENNSV
                     PQLRGDYKRGYDRLSGGLMLNQSVGFMYFGQNRLMNFTFSVEVVEAWVNPYRDYYFDL
                     MSPPPEGRQFDLLIGPKITWMLPLRQRNINKYYYY"
     gene            1156..2460
                     /locus_tag="PKZ74_09565"
     CDS             1156..2460
                     /locus_tag="PKZ74_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPM93047.1"
                     /translation="MWFLYDLIIKFFGCAVCIAALFNKKARKWMQGRKDFSSKLTDFT
                     TVQLSNCPTVVWFHCASLGEFEQGRPVIEAFREEHPDWKIIVTFFSPSGYEIRKNYPG
                     ADHIFYLPIDTPRNARNFIDLINPSLVIFVKYEYWFRYLDELYRRNIPVYFISAIFRD
                     NQHFFRWYGGWALKQLKKITGFFVQDDASAQLLRRHGIDRVTVSGDTRFDRVAAIAEN
                     AKAYPEVGKFVSGSPVFLAGSTWPADEEMILHLIEEAGYRVKFIIAPHEVGRERVEAL
                     REKVIGRQSSVASRRIESGTMEQWNNGTILFSELNGENAAGAGVLIIDGIGYLSHLYR
                     YATIAFIGGGFGVGIHNILEAATFGKPVIFGPNYHKFREAEELVEEGGAFPVENAQQL
                     NNRTIELLTDPVLLQKTSAICSAYVMKKKGATGRILSEIKLS"
     gene            2554..2988
                     /locus_tag="PKZ74_09570"
     CDS             2554..2988
                     /locus_tag="PKZ74_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HPM93048.1"
                     /translation="MKKYPVPDDQEELFFSSLNNKTGYDKISALREGIPYYAFERYAV
                     SSPLSLADWSEFLEISERSLQRYKKDNKTFDRMRSERIMEIAQVLKKGEEVFGDKEKF
                     TIWMNSNIIALGGIKPRELLESSYGIKLLDAELTRIDYGMFG"
     gene            2985..3449
                     /locus_tag="PKZ74_09575"
     CDS             2985..3449
                     /locus_tag="PKZ74_09575"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPM93049.1"
                     /translation="MIVFRLVRKKYGFALTGEGAKISGGRWNSVDLPVIYTSDSRALC
                     TAEVAVTLQRGILPNDFEMLSISIPDNILIHEIRTVDLPEGWRKFPYLEVTMQIGNRF
                     ILENRFMVMKVPSAVVPGDFNYLINPRHPDFEKIRIISTEPYEFDERFFSQR"
     gene            complement(3456..3998)
                     /locus_tag="PKZ74_09580"
     CDS             complement(3456..3998)
                     /locus_tag="PKZ74_09580"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM93050.1"
                     /translation="MNKKNSLMIPEEVIMSKIYLIRGKKIMLDKDLAELYEVKPIRLR
                     EQVKRNLQRFPANFLFRLTEEETVFMVSQNAIPSKQQLGGHLPYAFTEHGVLMLANVL
                     KSERAMQMSIRIIEIFVKIREMLLTHKDILLRLEQIEQTLSSHDDKVLLIFEYLKQLE
                     QAKQQELERNQRKRIGFKKG"
     gene            4264..5703
                     /locus_tag="PKZ74_09585"
     CDS             4264..5703
                     /locus_tag="PKZ74_09585"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPM93051.1"
                     /translation="MPKKISKIKHDDNLVLLTADVKNIGTDYLSKEEIEYAAVVVKDQ
                     KKETVLFDRLGKWMIVQVIKEEKCGHKRVENCRKAGDLLASRLNELKAKSVVVVDVDG
                     KGLETLALVEGMALGSYQFLKYKSEKERKEKENTLASVGILSKVIKDGQIDRLNIITG
                     AVLRCRDLVNEPVSHLTATVLSNEFRKMGEEAGIKVEVLNKNKIEALKMGGLLAVNKG
                     SAEPPTFTIMEWKPEKPVNKKPIILVGKGVVFDTGGLNLKTGAFMDGMKCDMAGAATM
                     ASALYTVARLNLPLHVIALMPATDNRLDATAVAPGDVIRMFDGTTVEVLNTDAEGRLI
                     LGDALSYARKYKPGLVITAATLTGAASRAIGRYGVVAMEKEATEEMTKLKASGDQVYE
                     RVAEFPFWDEYGELIKSDVADIKNIGGIDAGMITAGKFLAHFTDYPFIHLDIAGPAWI
                     EKKDSYRTAGGTGFGVRLLVEFLENKVSK"
     gene            5732..6892
                     /gene="pdxA"
                     /locus_tag="PKZ74_09590"
     CDS             5732..6892
                     /gene="pdxA"
                     /locus_tag="PKZ74_09590"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPM93052.1"
                     /translation="MEYTDERHRHDTDRVKVGITHGDMNGIGYEIIMKTFHDQRMLES
                     ITPVVYGSSKVASYHRKSLNISEINFNLVKNAASAIHRRMNIVNVIHDEVKIDLGKST
                     EIAGQLAFKALETATQDLLQRHIDVLVTAPINKKNMQTANFRFPGHSEYLSDKAGGKE
                     HLMLMVCDKLRVGVITGHIPLKDVPEALTTELILRKIELLHRSLIRDFGIRKPKIALL
                     GLNPHAGDLGVIGEEDQKVIAPAVEQAWNKNIIVYGPYSADGFFGSNSYVKFDGILAM
                     YHDQGMIPFKTLSFDRGVNFTAGLPFIRTSPAHGTAYDIAGKNLASHVSFREAVYLAI
                     DIFNNRKEFDELNNKPLQNFLKDIESNSDAVIEKEIPGAADEAANGGLVAGQ"
     gene            6917..>7259
                     /locus_tag="PKZ74_09595"
     CDS             6917..>7259
                     /locus_tag="PKZ74_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPM93053.1"
                     /translation="MEYSISEIVSIVNGNPSSSPFIERNIRDILIDSRKLIAPEQCLF
                     FALKSKRNDGHHYIPELYGKGIRDFVVSRLPADPEAYPEANFILVKNTLHALQALARK
                     HREKFRIPVVGI"
BASE COUNT         1898 a         1668 c         1906 g         1787 t
ORIGIN      
        1 ggcgacgaag gtgaaggaga cagtgatgac ctcgtcgccc ggtttcaggt cgagggccat
       61 catggaaacc tggagggcgt cggttccgtt agcgcagggt ataacatgct taatgccaag
      121 gtattgctcc aggttcttct ggaaacgttg cacggcaggg ccgttaataa aggaagtcgt
      181 gtcgatcact tcgtggatgg ctgcatccac ttcgggtttg attttgtcgt actgggtttt
      241 caggtccacc atccggatag gtcgcataga gaggatattt aatataagta aatgttttaa
      301 ctggtgcgca aaggtagtga aaatggtgaa cggtaagcgg tgaagggtga agggaattgt
      361 tatctttgtg aaaaaataca catgcgcttt gtttcgattg ctgtactggt cattatcact
      421 cagggccttc tcgcccaggt cgaggtaaat gaagaagatt cagctttgag tatcccgatg
      481 ttttatggct tttacggcta ccagtggccc ggtggcgacc tggatgaaca attcgggtca
      541 aactcggtgg tcgggcccgg ttttttatgg aaaacgaaat cgaactggct gttcggggca
      601 gagtatgatt tccatttcgg gaataatgtg aagaatacaa aagagatatt taagaatatt
      661 tacaccacga atggcaatgt gataagcggc gacggggtgc cggctttggt ggcaacgttc
      721 gagcggtgca acagtttcag cgccaggttc gggaagatca tcccggtatc ggaaaatgac
      781 aggaattcag gtattttctt caccgtcggc gcaggatatt tatcacacaa gatcaaaata
      841 gaggtcgaga ataacagtgt cccgcaactg cgcggtgatt ataaaagagg gtacgaccgg
      901 ctcagtggcg gtttgatgct gaaccagtcg gtcgggttca tgtatttcgg ccagaacagg
      961 ctgatgaatt tcactttttc agttgaggtt gtggaggcct gggtgaatcc ttatcgcgat
     1021 tattattttg atctgatgtc gcctcccccg gaaggaaggc agtttgacct gctgatcggg
     1081 ccgaagatca cctggatgct gccattgcgc cagcgaaaca tcaataaata ttattattat
     1141 taattctttg gcaggatgtg gttcctctac gacctgatca taaaattttt cggttgtgca
     1201 gtatgtatcg ccgccttgtt caacaaaaaa gcacggaagt ggatgcaggg cagaaaagat
     1261 ttttcttcta agctaaccga ttttacaacc gtccaattgt ccaattgtcc aactgtcgta
     1321 tggttccact gcgcatcgct gggcgagttc gagcaggggc ggccggtgat cgaagcattc
     1381 cgcgaggagc accccgattg gaaaatcatc gtgacattct tttcgccttc agggtacgag
     1441 atcagaaaaa attatccggg agccgatcac atcttttatc ttcctatcga tacgccccgc
     1501 aatgccagga acttcatcga cctgatcaat ccttcgcttg tcatctttgt taaatacgag
     1561 tactggttcc gttacctgga tgagctttac cgacgcaaca tccctgtcta tttcatttca
     1621 gccattttcc gcgataatca gcattttttc cggtggtatg gaggatgggc gctgaaacaa
     1681 ctgaaaaaga tcaccgggtt ttttgtgcag gatgatgcct ctgcgcaatt acttcgcagg
     1741 catggcatcg accgggttac ggtcagcggc gacacccggt tcgaccgggt agcagcgatc
     1801 gcggagaatg cgaaagcgta tccggaagtc gggaaatttg tgtcgggaag cccggtgttc
     1861 ctggcaggca gcacctggcc ggccgatgag gagatgatcc ttcaccttat cgaagaggca
     1921 gggtacaggg tgaagttcat catcgcgccg cacgaggtgg gcagggagcg ggtggaggcg
     1981 ctgagagaga aagtcatcgg tcgtcagtcg tcggtcgcca gtcggcgaat tgaatctgga
     2041 acaatggaac aatggaacaa tggaacaatc ctgttttctg aactcaatgg tgaaaatgca
     2101 gccggcgccg gtgtactcat catcgacggg atcggttatc tttcccattt gtaccggtac
     2161 gccaccatag catttattgg cgggggattt ggcgtgggta tccataatat tctggaggct
     2221 gccacatttg gcaagccggt aatcttcggc ccgaattacc ataaattccg cgaagcggaa
     2281 gaacttgttg aagaaggcgg tgcatttccg gttgaaaacg cgcaacaatt gaacaacaga
     2341 acaattgaac tcttaaccga tccggtactt ttacagaaaa cttcggcgat ttgttcagcc
     2401 tatgtcatga aaaagaaagg ggctaccggc aggatccttt ctgagatcaa actttcttag
     2461 cgcctctgcg cctccatgtc tccgcgatca agttaatacg acatatgtca tcattattca
     2521 gacaaatgtc gtacatttgt aaaaactagt tccatgaaaa agtacccggt tccggatgat
     2581 caggaagaat tatttttttc ttccttaaac aataaaacag gatatgataa aatttctgct
     2641 ttgcgtgaag gcattccgta ctatgcattt gaaagatatg ccgtttcaag ccctttatca
     2701 ctggctgact ggtcggaatt cctggaaatt tccgaacgga gcctgcagcg gtataagaag
     2761 gataacaaga ccttcgatcg gatgcggtcg gaaaggatca tggaaatcgc ccaggtattg
     2821 aagaaaggcg aggaggtgtt cggtgataag gagaagttca cgatatggat gaacagcaac
     2881 attatcgctc tagggggtat caagccccgg gaactgcttg aaagcagcta tggaattaag
     2941 cttttagatg cagaattgac aaggattgat tacgggatgt tcggatgatt gtatttaggc
     3001 ttgtcaggaa gaaatatgga tttgcgctta caggagaagg tgcaaagatt tccggtggga
     3061 ggtggaacag tgttgatctt ccagtgattt acaccagtga ttccagggcg ctttgcacgg
     3121 cagaagttgc tgttaccctt caaaggggta ttctgccgaa tgattttgaa atgttgtcga
     3181 tttcgatccc tgataacata ttaattcatg agatacgtac tgttgatctg ccggaagggt
     3241 ggaggaaatt tccttatctt gaagtgacga tgcagattgg caaccggttc attcttgaaa
     3301 acaggttcat ggtcatgaaa gtgccatctg cagtcgtacc aggtgatttt aactacctga
     3361 tcaatccccg tcatcctgat tttgaaaaaa taaggatcat cagcacagag ccttatgaat
     3421 ttgatgaaag gtttttcagc cagcgttagt cgtgttcatc cctttttaaa gccgatccgc
     3481 ttgcgctggt ttcgttcaag ttcctgttgt ttggcttgct ccaactgttt gagatactcg
     3541 aagatcagca ggaccttgtc gtcatggctg gagagggttt gttcgatttg ttccagcctc
     3601 aataaaatat ctttatgcgt gagcagcatc tcccggattt taacaaagat ttcaatgatc
     3661 cggatgctca tttgcatggc ccgttcactt ttcagaacgt tggccagcat taacacgcca
     3721 tgttcggtaa acgcatacgg taaatgacct ccgagttgct gttttgaagg tatcgcattt
     3781 tgcgatacca taaaaactgt ttcctcttct gttaacctga acagaaagtt agcaggaaaa
     3841 cgctgcaggt ttcttttaac ctgttcacgc aaacgaatgg gttttacttc gtaaagttct
     3901 gccagatcct tgtccagcat gatctttttt cctcgaatca ggtaaatttt gctcatgatc
     3961 acttcctcag ggatcatcag tgagttcttt ttattcatag gtacgggttt tcgcgtgaaa
     4021 taataatgtt aaagaaattg gaggtcgcaa attgcgtccg ccatcccatc ttattttaat
     4081 ccgttttttt cgagaaaaat acccggccgg cgtaaaatat tttgataagt agctggtata
     4141 gaagggataa ggggttggct cattgagact ccatctgctt aatttgagat ggagtctcat
     4201 agaaattgag atggagtctc agtttcttac ttacttttgt ccgttattct taaaacgaac
     4261 cctatgccga aaaagatcag taaaataaag cacgacgata acctcgtcct gctgaccgcc
     4321 gacgtgaaaa atatcggcac ggattatctt tcaaaggaag aaattgaata tgcagccgtg
     4381 gttgttaagg atcagaaaaa ggaaacggtg ctgttcgacc gcctcggaaa atggatgatc
     4441 gtccaggtga tcaaagagga aaagtgcggt cacaaacggg ttgagaactg ccgcaaggcc
     4501 ggcgacctgc tggcttccag gctcaacgag cttaaggcga aaagcgttgt ggtagtggat
     4561 gttgacggaa aagggttgga aacccttgcc ctggtcgaag ggatggcgct gggcagctac
     4621 cagttcctga aatataagtc ggaaaaagaa cggaaagaga aagagaatac gctggcttcg
     4681 gtcggtatcc tgtcgaaagt gataaaagat gggcagatcg accggctgaa catcatcacg
     4741 ggagcagtgc tccgttgccg cgacctggtc aacgagccgg tgtcgcacct caccgctacg
     4801 gtcctttcga acgagttcag gaagatgggc gaggaagcgg gaatcaaggt ggaggtgctc
     4861 aacaaaaaca aaatcgaagc gctgaaaatg ggtggcctgc tggccgtcaa caaaggtagc
     4921 gccgaaccgc cgaccttcac gatcatggaa tggaaaccgg aaaagccggt gaataaaaag
     4981 cccattatcc tcgtaggcaa aggggtggtg ttcgacacgg gcggactgaa cctgaaaacg
     5041 ggcgccttca tggacgggat gaaatgcgat atggccggcg ccgccacgat ggcttccgcc
     5101 ctgtacacgg tagccaggct gaacctgccg ctccacgtca tagccctgat gccggctacc
     5161 gacaaccgcc tcgacgccac cgccgtcgcc ccgggcgacg tgatccggat gttcgacgga
     5221 acaaccgtcg aggtgcttaa caccgatgcc gaagggcgcc tgatcctggg cgacgcgctg
     5281 agctatgccc gcaagtacaa accggggctg gtgattaccg ccgctaccct caccggggcc
     5341 gccagccgtg ctatcggccg gtatggcgtg gtggccatgg agaaggaggc cacagaagag
     5401 atgacgaaac tcaaagccag cggtgaccag gtttatgaac gggtggcgga atttcctttc
     5461 tgggacgaat acggcgaact gatcaaatcg gatgtggccg atatcaaaaa catcggcgga
     5521 atcgatgccg gcatgatcac cgcaggcaaa ttcctggccc atttcaccga ctatcctttc
     5581 atccatctcg atattgccgg cccggcctgg atcgagaaaa aagacagcta ccggaccgcc
     5641 ggcggaacag gattcggtgt caggctgctg gttgagtttt tagaaaacaa agtgagcaaa
     5701 taaaaattac atttgcttaa atttttcagg gatggaatat accgacgaaa gacacaggca
     5761 tgataccgac cgcgttaagg tcgggatcac tcatggcgac atgaacggca tcggctacga
     5821 gatcatcatg aaaactttcc acgaccaaag aatgctcgaa tcgatcacgc cggtcgttta
     5881 tggctcgtcg aaagtagcct cctaccatag gaagtcgtta aatatcagtg agataaactt
     5941 caaccttgtc aagaatgccg cttcggccat tcaccggagg atgaacatcg tcaacgtgat
     6001 ccacgatgag gtgaagatcg acctggggaa atcgacggag atagccggac aactggcttt
     6061 taaagcgctg gaaacggcca cgcaggatct gctgcaacgc catattgatg tcctggttac
     6121 cgccccgatc aataagaaaa acatgcagac agccaacttc agattcccgg gccacagcga
     6181 atatttgtcg gataaagccg gcggaaagga acacctgatg ctgatggtct gcgataagct
     6241 ccgggttggt gtcattaccg gccatattcc cctgaaggat gtcccggaag cattgactac
     6301 cgagctgatc ctgcgcaaga tcgaactatt gcaccggagc ctgatccgcg atttcggcat
     6361 ccgcaagccg aagatcgcgc tgctggggct gaaccctcac gccggcgacc tgggagtgat
     6421 cggggaggaa gaccagaaag ttattgcacc tgccgttgag caagcctgga ataaaaatat
     6481 catcgtttac ggtccttatt ccgctgacgg tttcttcggt tccaacagct atgtgaaatt
     6541 cgacggcatc ctggccatgt accacgacca ggggatgatc ccgttcaaaa cattgtcgtt
     6601 cgaccgcggc gtcaatttta cggccgggct gccattcatc cggacatcac cggcccatgg
     6661 cacagcctac gatatcgccg gcaaaaacct ggcctcccac gtttctttcc gcgaagcggt
     6721 ttacctggct atcgatattt tcaataaccg gaaagaattc gatgagctga acaacaaacc
     6781 gctgcagaat tttctcaaag acattgaatc gaattccgac gcagttatcg aaaaagagat
     6841 ccccggggct gccgacgaag cagccaatgg cggcctggtg gcaggccagt aaaactatct
     6901 atcttcttac ccttttatgg agtacagtat ttccgaaatc gtcagcattg tcaatggcaa
     6961 tccttcctct tcccccttta tagaaaggaa tatccgggat atcctgatcg acagccgtaa
     7021 gctgatagcg cccgaacaat gccttttttt cgccctcaaa agcaaacgga acgacggtca
     7081 tcattatatc ccggagcttt acgggaaagg gatccgcgac tttgtcgtca gccgtcttcc
     7141 ggccgatccg gaagcttatc ctgaagccaa cttcattctt gtcaaaaata cgctgcatgc
     7201 ccttcaggcg ctggccagga aacaccgcga gaaattccgg atacccgtcg tcgggatta
//
LOCUS       DAOPNH010000171         7210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11737_length_7210_cov_6.007687, whole genome shotgun sequence.
ACCESSION   DAOPNH010000171 DAOPNH010000000
VERSION     DAOPNH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_7210_cov_6.007687"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..257)
                     /locus_tag="PKZ74_09600"
     tRNA            complement(170..257)
                     /locus_tag="PKZ74_09600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Ser,seq:gga)
     gene            complement(391..1431)
                     /locus_tag="PKZ74_09605"
     CDS             complement(391..1431)
                     /locus_tag="PKZ74_09605"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HPM93054.1"
                     /translation="MSYSKPSILIIYTGGTIGMVRDRETGSLHPVSGTELYEHIPMIG
                     RMDYVIDFYSFDPLLDSSNINPGHWIDLVEVIAGHYDKYDGFVVLHGSDTMAYTASAL
                     SFMLENLNKPVILTGSQLPLGVIRSDGRENLVTAIEIAAAQEDETPVVPEVAIYFENM
                     LMRGNRTFKYNAEHFEAFRSGNYPALAEVGVHIRYNRNAILKPNFRMLKAHTTLDNHI
                     TILKLFPGISPESVRAQLSIPGLRAIVLETYGSGNATTEGWFTGMLAAAIKEGMIILN
                     VTQCKAGSVEIGKYQTSRELGQIGVLSGYDITTESAVTKLMYLLALGLEKSETEKLLQ
                     LSLRGEMTIGRV"
     gene            complement(1515..2282)
                     /locus_tag="PKZ74_09610"
     CDS             complement(1515..2282)
                     /locus_tag="PKZ74_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPM93055.1"
                     /translation="MILTDTHAHLYLEQFDADRHDAVRQAIAAGVNYILLPNIDKDSI
                     EPMMGLANAFPGHCFPMMGLHPTSVRADYREQLNVVESWLQKGKFCAVGEMGIDLYWD
                     KTFLREQQEAFRIQARMAKDHNLPLVIHSRESFSEIFRLMDEVYEPGLRGVFHCFTGN
                     REQAERIISMGFYLGIGGVLTYKNSNLPEVLREIPAEYLLLETDAPFLAPVPYRGKRN
                     ESAYIPEIAGKLAEVKHIETDELARITTNNAKKLFKL"
     gene            2338..3306
                     /locus_tag="PKZ74_09615"
     CDS             2338..3306
                     /locus_tag="PKZ74_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPM93056.1"
                     /translation="MKKILFIRFSSIGDIVLTTPVIRCVKQQVQGAEVHVAVKKAFLP
                     VVSANPYIDKIHVLGDDLKALTGLLRKERFDFIADLHCNIRSFYIRLSLHRPSAGFPK
                     LNFRKWLLTRFHINRMPGIHIVDRYFRAVEPLGVKNDGKGLDYFIPDDEEIGMASLPA
                     EFRKGYTAFVIGAKHATKKLPEHKIISICRKMNSPVILLGGPEDAPAGDRIAAACGPR
                     VMSMCGKATLNGSASLVKQADAVITHDTGLMHIAAAFRRRIVSVWGNTVPELGMYPYL
                     PGDEERSLIVEVKGLRCRPCSKLGYDRCPKGHFRCMEEINDSSFTS"
     gene            complement(3372..4016)
                     /locus_tag="PKZ74_09620"
     CDS             complement(3372..4016)
                     /locus_tag="PKZ74_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM93057.1"
                     /translation="MLNIHRFSFNPFQVNTFILWDETRECAIVDPGCYSKAEQDELRD
                     FIRENGLKPVRLINTHCHIDHISGVAFVSREYGLKPEAHFDGIPFIRHSEKTSFIYGF
                     DSLETTDPQLHLKEGDIIRFGNSELEVVEAPGHAAGSVCFISREGKFVITGDVLFHQS
                     IGRTDLPTGDYDLLIRNIREKLLTLPPDYTVYAGHGPETTIGYEMYSNPFLEKE"
     gene            4140..>7210
                     /locus_tag="PKZ74_09625"
     CDS             4140..>7210
                     /locus_tag="PKZ74_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93058.1"
                     /translation="MFWAAFAVFMALLLARPSSAQFYNGSQMSFGKNRIQYKEFLWTY
                     YKFEDFDIYFYLNGMELAVATAKYATEQIPLMEKKLQTSLDQKIQFLVYNNLTDLKQS
                     NIGLMQDERYNTGGITYIIDSKAFIYFSGEHRDFERQIRAAIANILINQAFYGTSVGS
                     QVKNSTLFTLPEWYRNGLVSYLSEDWNTEIDNKVKDGLLSGRYKRLNTLVGEDALYAG
                     HMLWKYIADKYGTQALTDIVSMTQISRSVDNGFLYVIGLSYKSLVAECLAHYTKIAEA
                     EEAGRSTPDESFLKKIRKRSVYSEFTFSPGKMFAAYAENDEGKYKVFLQNLRTGKKFS
                     VLRGGFRLEEKIDYSYPLITWHPDGNTLSIIVEKRGEVYLYSYNIEERKRNFIILYEF
                     EKITDFSYSPDGRYLVMSAVQKGQSDLYLYNIAATSLQQLNNDIYDDLNPVFAEGTGQ
                     ILFSSNRPDEKLAWMDYEDAPAAVGKTTDLFLMIRGDNKNILRRVTNTPLGSELRPEM
                     TDKGRFMFLSDENGINNRYFGQFDSAISFIDTTIHYRYFFETYPATNYSRSILSHEID
                     TVSGSLSQVVFQRGKYHLQEEELFPGTPDSVSLSQTAYRTVAVIEQEVKKEEEKAETE
                     LESYGEYTRRFRNVYRKDEQPAQPPPSDNSRIDIDNYVFEPTVTVTERGQDTVKQQPS
                     PDPKTKIEVKEGFVIPKRLNYRVEYTINQMGAQLDFTALNYFYQPFSGGSGLGISNLG
                     TNGNGFFQVGLNDLLENHRITGGFRVPLGISNVEYMFSYSDIEKRLDKEWLVVRQAIE
                     NQYTDGIYTDIIRFRSYQLFYMLKYPFSPVFAIKGTANVRYQKSTYLSTSEATLRAPD
                     SVEFWGGVKGELIYDDTKDIAPNLMTGTRFKIFGEYTQVLSEDSKGLFVVGLDVRHYL
                     RIHRSFIWANRLAASTSFGRNKLIYFMGGVDNWYRPKFSTEAPIDYSMNYTYQAIATN
                     MRGFKQNARNGNNFVVLNSELRFPVVRYFFNRPVKSDLLSNFQVVAFGDVGL"
BASE COUNT         1829 a         1750 c         1804 g         1827 t
ORIGIN      
        1 gattctgtca cctgggttgt ctgtacagat tgatcgtcac tttgtccata agcatacgcc
       61 tgattgaata cccccgtcgt aagaagggca gccagagcta aaaatgtgat taagcgtttc
      121 atagtttaag ttattgttaa ttaatcttgt tttggtttca taaataatgc ggagagaatg
      181 ggattcgaac ccatgaaccg cttttgacgg ttacacactt tccaggcgtg cgccttcgac
      241 cgctcggcca tctctccata tttcaaaatt ggtctaactc tccaaaatcg tgcgctaaag
      301 taataaaaaa acaattcaaa aaggtctgaa tataaatatt tataagcaat ctaatctgcg
      361 tttaatgttt cattctcagg gtcttcatga tcaaacccgg ccaattgtca tttcccccct
      421 taacgataat tgcaataatt tttccgtttc actcttttcc aggccaagcg ccagcaggta
      481 cataagcttg gtcacagccg attctgtagt aatatcgtat ccgctcaata cccctatctg
      541 tcctaactcc cggctggtct gatatttgcc tatctcaacc gaaccggcct tgcactgggt
      601 gacatttaat atgatcatac cttctttaat agctgccgct aacataccgg tgaaccaccc
      661 ctcagtcgtg gcatttccgg agccgtaagt ttccagcaca atcgcccgca ggcccggaat
      721 agataactgg gcccggaccg attccggcga tataccgggg aataatttca ggatggtgat
      781 atgattgtcg agtgtggtat gggccttcag catcctgaaa ttgggtttca ggattgcatt
      841 ccggttatat cggatatgaa cgccgacctc ggccagcgcc gggtaattcc ctgaccggaa
      901 cgcctcgaaa tgttcggcgt tgtacttgaa agtccggttg ccgcgcatca gcatgttctc
      961 aaaataaatg gctacttcag gcacaaccgg ggtctcatct tcctgggccg ctgcaatctc
     1021 tatggctgta accaggtttt ccctgccgtc gctgcggatc acccccaagg gcagctgcga
     1081 tccggtcagg atgacaggct tgttcaggtt ctccagcata aaactcagcg ccgacgctgt
     1141 atatgccatc gtatcagatc cgtggagtac aacaaatccg tcgtacttat cgtagtggcc
     1201 ggcaataact tccaccaggt cgatccagtg cccgggattg atattcgatg aatcgagcag
     1261 cggatcgaaa gaataaaagt cgatcacata atccatcctg ccgatcatgg gtatgtgctc
     1321 atacagttcc gttccgctga ccgggtgaag actgcccgtt tcccggtcgc ggaccatgcc
     1381 gatcgtcccg ccggtgtata tgatgaggat gctgggtttg gaataagaca cagggaggaa
     1441 aatttaatgt ttaattctta attttaaatt ttatttgtta gtggttgaat atcaattaat
     1501 aataaatgat gttgttataa cttaaataat ttctttgcgt tgttggtggt gatccgggcg
     1561 agttcatccg tctctatatg ctttacttcg gccagtttac cggctatttc ggggatatag
     1621 gcgctttcgt tccgtttgcc ccggtatggg acgggggcaa ggaaaggagc atccgtttcg
     1681 agcaaaagat attcggccgg aatttctctt aagacttccg gcagattcga atttttatac
     1741 gtcagcaccc cgccaatgcc caggtaaaat cccatagaaa tgattctttc agcttgctcg
     1801 cggttgccgg taaaacaatg aaacaccccc ctcaggccgg gttcgtaaac ttcatccatc
     1861 agccggaaaa tttcgctgaa cgattcgcgg gaatggatga ccagcggcaa attatgatcc
     1921 tttgccattc gggcctgtat cctgaaagct tcctgctgct ccctcaggaa ggttttatcc
     1981 cagtaaagat cgatccccat ctcaccgacg gcacagaatt ttcctttttg tagccaggat
     2041 tcgaccacat tcagttgttc ccggtaatcg gctctgaccg aggtgggatg gagccccatc
     2101 atcgggaaac agtgccccgg aaatgcattc gccaggccca tcatcggttc aatactgtcc
     2161 ttgtcgatat tgggaagcag aatataattc actcccgcgg caatggcctg tctgacggca
     2221 tcgtgacggt cggcgtcaaa ctgttccagg tagaggtgcg catgggtatc ggtgaggatc
     2281 atgtggcaaa attagctgaa attgctgctg aagtcttact tttgtaaaaa atcggccttg
     2341 aaaaagatcc tcttcatccg cttcagctcg atcggcgata ttgtgctgac cacgcccgtg
     2401 atccgttgtg ttaaacaaca agttcagggc gccgaagtcc atgtcgccgt aaagaaggcg
     2461 ttcctcccgg tcgtatcggc caatccgtat atcgataaaa tccatgtgct gggtgacgat
     2521 ctgaaggctt tgaccggttt actaagaaag gagcgctttg atttcatcgc cgacctgcat
     2581 tgcaatatcc ggtcgtttta tattcgtctc tcactgcatc gtccatcggc cgggtttccc
     2641 aaactgaatt tccgtaaatg gttgctgacc cgtttccata tcaaccgcat gcccgggatt
     2701 cacattgtcg accggtattt cagggccgtg gagccactgg gcgttaaaaa cgacgggaaa
     2761 gggctggatt atttcattcc cgatgacgag gaaatcggga tggcgtccct gccggcagag
     2821 ttccggaaag gctacacagc atttgtcatc ggcgccaaac atgccaccaa gaaactgcct
     2881 gagcacaaga tcatttccat ttgccggaaa atgaacagcc cggtaatcct tctgggcggg
     2941 ccggaagatg cccctgcggg cgacaggatt gcagccgcct gcgggccacg cgtcatgtcg
     3001 atgtgcggaa aagcaaccct taacgggagc gcgtcactgg taaaacaggc cgatgccgtc
     3061 atcacccacg atacaggcct gatgcatatt gccgccgctt tccgcaggcg gatcgtctcg
     3121 gtatggggaa atacggtgcc ggaactgggg atgtacccgt atttgcccgg tgatgaagag
     3181 agatcattga tcgttgaggt gaaaggcctt cgctgccggc cctgctccaa actgggctac
     3241 gaccgctgcc cgaaagggca tttccggtgt atggaggaga ttaatgattc ttcgttcacg
     3301 tcataagttc acttcgttca cgtgattagc tcgctgcgct cgcgtcattg gtcaggaaag
     3361 gaatcctaaa cttactcctt ttccaggaag ggattactgt acatctcgta accgatggtt
     3421 gtctcggggc cgtgccctgc gtaaacggtg taatcgggcg gcagggtgag cagtttctca
     3481 cggatgttcc ggatcagtaa atcgtaatcg cctgtcggca gatcggtccg cccgatgctc
     3541 tggtggaaaa gaacgtcacc agtgatcacg aatttgcctt cacggctgat aaaacacaca
     3601 ctgccggcgg catgcccggg ggcctcaacc acttccagtt cactgtttcc gaatcggata
     3661 atatcaccct ctttcaaatg taattgcgga tccgttgttt ccagagagtc gaagccatag
     3721 atgaaactgg tcttctccga atggcggata aacgggatcc cgtcgaaatg ggcctccggt
     3781 ttcagcccgt attcccggct tacaaaggcc acaccggaga tgtgatcgat atggcagtgt
     3841 gtattgatca gcctgaccgg ttttaatcca ttttccctga taaaatccct gagttcatcc
     3901 tgttccgctt tgctgtagca ccccggatct acgatagcgc actctctggt ttcatcccag
     3961 aggatgaatg tattgacctg gaaagggttg aatgaaaagc gatgtatgtt gagcatatcc
     4021 ttaatacatt aagagcgggc aaagaaacaa cattttcccg attttttagt ttattttagt
     4081 ggctgatttc aggctgtaca gacaggatgg ctaaaagctt tacaacgaat aagttacgat
     4141 tgttttgggc agcgtttgcg gtgttcatgg ccctgttgct tgccaggccg tcctctgccc
     4201 agttttacaa cggcagccag atgagtttcg ggaagaaccg gatccagtac aaggagttcc
     4261 tgtggaccta ttacaagttc gaggatttcg acatctattt ttacctgaac gggatggagc
     4321 ttgccgtggc aacggcaaag tatgccacag agcaaatccc gctgatggag aagaaattgc
     4381 agaccagcct cgaccagaag attcagttcc tggtttacaa taatctcacc gatctgaaac
     4441 agagcaatat aggccttatg caagacgagc ggtacaatac gggtggtatt acctatatta
     4501 tcgacagcaa agcctttatc tatttcagtg gcgaacaccg cgattttgaa cgccagatca
     4561 gggctgccat tgccaatatc ctgatcaacc aggccttcta tggcaccagt gtcggttcgc
     4621 aggtgaaaaa ttctaccctg ttcaccctgc cggaatggta ccggaacggg ctggtatcct
     4681 atctctcaga ggactggaat acggagatcg ataataaagt gaaagacggg ttactgtcag
     4741 gtcggtataa gcggctgaat acgctggtcg gtgaggatgc actctatgcg ggacatatgc
     4801 tctggaaata tatcgccgat aaatacggta cgcaggcttt gaccgatatc gtcagcatga
     4861 cccagatcag ccggagtgtc gacaacggtt tcctgtacgt cattggttta tcctacaaaa
     4921 gcctggtggc cgaatgcctg gcacattata cgaaaattgc ggaggcggaa gaagccggga
     4981 ggtcgacccc ggatgagagc ttcctgaaga agatcagaaa gagaagcgtt tatagcgaat
     5041 ttacattcag ccccgggaaa atgtttgccg cgtatgccga aaatgatgaa ggaaaatata
     5101 aggtgttcct ccagaatctc cgcaccggaa agaagttttc tgtgctgcgc ggcggattcc
     5161 ggctcgagga aaagatcgat tattcctatc ctttgatcac gtggcatccc gatggcaata
     5221 cgttaagtat catcgtggag aaaaggggag aggtatatct ttattcctat aatattgagg
     5281 aaaggaaacg aaactttatc atactctatg aatttgaaaa gattaccgat ttttcttatt
     5341 cacccgacgg ccgttatctg gttatgtcgg ctgttcagaa aggacagtcg gacctgtatc
     5401 tttataatat tgccgcaaca tctctccagc agctcaacaa tgatatttac gacgacctga
     5461 atcccgtttt tgcggagggg acggggcaga tccttttttc ttccaaccga ccggacgaga
     5521 agctggcctg gatggattac gaagacgctc cggcagcggt gggcaaaacc accgatctct
     5581 tcctgatgat ccggggcgac aacaaaaaca tcctcagaag ggtcaccaac acccccctgg
     5641 gaagtgagct gcgaccggaa atgacagaca agggcaggtt tatgttcctg agcgacgaga
     5701 atgggatcaa caaccggtat ttcggacagt tcgacagcgc catcagtttt atcgatacaa
     5761 ccattcatta ccgttacttt ttcgagacct acccggcgac caattattcg aggagtatcc
     5821 tcagccatga aatcgatacg gtttcaggat ccttgtccca ggttgtcttc cagagaggta
     5881 aatatcattt gcaggaagag gaattgtttc cgggcacacc cgattcggta tcgctgagtc
     5941 agacagccta ccggaccgtt gccgtcattg agcaggaggt taaaaaggag gaagaaaaag
     6001 cggaaaccga attggagagt tacggcgagt atacacgccg gttccggaac gtttaccgga
     6061 aggatgaaca gccggcgcag ccccctcctt ccgacaacag ccggatcgat atcgacaatt
     6121 atgtatttga accgaccgtg acagtgacgg agagagggca ggatactgtc aaacaacaac
     6181 cttcgcccga tccaaagacg aaaattgaag tgaaagaggg ctttgttatt cccaagcgac
     6241 ttaattacag ggttgaatat acgatcaacc agatgggagc ccaactggac tttaccgctt
     6301 tgaattattt ctaccagccc tttagcggtg gcagtggcct ggggatatcc aacctgggga
     6361 caaacgggaa cggctttttc caggtcggcc tgaatgatct tcttgaaaat cacaggatca
     6421 caggcggttt cagggttccg ttaggcatct ccaacgtgga atacatgttc agttattccg
     6481 atatcgaaaa gcggctcgac aaggagtggc tggtcgtaag gcaggccatt gaaaaccaat
     6541 acacggatgg aatttatacc gatatcatcc gcttcaggtc gtaccagttg ttttacatgc
     6601 tgaaatatcc tttttccccg gtatttgcca taaaaggcac ggcgaatgtc aggtaccaga
     6661 aatcgactta cctgtcgacc agtgaagcca ctttaagggc tcccgacagt gttgaattct
     6721 ggggaggcgt gaaaggagag ctgatttacg atgatacaaa agatatcgca ccgaacctga
     6781 tgaccggcac ccggtttaaa attttcggcg aatacacaca ggttctcagt gaagacagta
     6841 aaggtctgtt tgtggtaggt cttgatgtgc gccattacct gcgaatccac cggtcgttca
     6901 tctgggcaaa ccggctggcg gccagtacat ctttcggcag gaacaagctg atttacttta
     6961 tgggaggcgt cgataactgg tacaggccca aattttccac ggaagccccg atcgactatt
     7021 ccatgaatta tacctaccag gcgatagcta cgaatatgcg cggattcaaa cagaatgcgc
     7081 ggaatggaaa taatttcgtg gtactgaaca gcgaactgag attcccggtg gtcaggtatt
     7141 tctttaacag gccggtaaaa tcagacctgc tcagcaattt ccaggtcgtg gctttcggcg
     7201 atgtcggcct
//
LOCUS       DAOPNH010000172         7203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11754_length_7203_cov_5.704113, whole genome shotgun sequence.
ACCESSION   DAOPNH010000172 DAOPNH010000000
VERSION     DAOPNH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11754_length_7203_cov_5.704113"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1386
                     /locus_tag="PKZ74_09630"
     CDS             181..1386
                     /locus_tag="PKZ74_09630"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPM93059.1"
                     /translation="MPHYQRLGLIPPKRHIQFRKPDGALYSEQLVSTEGFSAEYSLVY
                     HVYPPTVVTKIDKPFSVAPEIAVDKNMQHRSFQGFKVAPQDNYINSRIPVLVNDDCYI
                     HLAAPKSWAGQQSTRTMENGQSTIDNLIFFKNSDAHELIFIHQGEGTLKTMYGSIPFR
                     YGDHLIIPRGVIYRLDFNSEENRLFIVESFSPFRFPKRYVNRMGQLLEHAPFYERDIR
                     KPENLETHDAKGDFIVMVKKQDLVYPYHYASHPFDVIGWDGYHFPYALSIHDFEPITG
                     RIHQPPPVHQTFEANNFVMCAFVPRLYDYHPEAIPVPYNHSNVDSDEVLYYVDGEFMS
                     RKHVEKGQITLHPIGIPHGPHPGTVEKSLGQKETRELAVMVDTFRPLKITKQAMEIED
                     SEYFRSWAE"
     gene            1440..2534
                     /gene="hppD"
                     /locus_tag="PKZ74_09635"
     CDS             1440..2534
                     /gene="hppD"
                     /locus_tag="PKZ74_09635"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HPM93060.1"
                     /translation="MSTDFLPLNGTDYVEYYVGNAKQAAYYYMTAFGFQPLAYSGLET
                     GNKEKTSYVLVQDKIRFVFTTSLVPDSEIGEHVKKHGDGVKVVALWVDDATSAFNETV
                     KRGAKPYLDPVKEQDAMGHVVRSGIHTYGETVHIFVERKDYRGAFLPGFSKWEPAYRT
                     EPIGLKYVDHMVGNLGWGEMNKWAKFYEDVMGFKQLISFDDKDISTEYTALMSKVMSN
                     SNGYIKFPLNEPAEGKKKSQIEEYIDFYGGAGVQHVAMATDNIIHTVSELKKRGVEFL
                     YVPGTYYEDVLDRVGHIDEDLKPLKDLNILIDRDDEGYLLQIFTKPVEDRPTLFYEII
                     QRKGAKSFGKGNFKALFESIEREQARRGTL"
     gene            2595..3836
                     /gene="fahA"
                     /locus_tag="PKZ74_09640"
     CDS             2595..3836
                     /gene="fahA"
                     /locus_tag="PKZ74_09640"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HPM93061.1"
                     /translation="MYTSWINIPPENDFSIFNIPFGIISYKGYSPRPATRIGDTVVDL
                     AALAGYGFFDGLGISDLSVFNRPALNDFIALGKPVTRAVRDRLISLFRSDNPELRDHS
                     EARRTSLLNAVDVTMHLPVKVGNYTDFYASEQHAYNVGVMFRDPKNALLPNWKHIPVG
                     YHGRASSIVVSGASIHRPMGQTKADDQEFPAFGPSKQVDFELEMGFVIGKSSALGDRV
                     KISDAEDYIFGLVLFNDLSARDIQKWEYVPLGPFLSKNFGSVISPWIVTTEALEPFRV
                     PGPVQVPEVLPYLKYQGNHHFNIKLEVMLQGKLLTVTNSKHLYWNFAQMLAHHTVNGC
                     NVEVGDIYGSGTISGPEQGSYGSMLEIAWKGTKPVVMPDGTSRSFIHDHDTIVIKGFG
                     EKQGIRVGFGECKVTILPAKK"
     gene            3909..4796
                     /locus_tag="PKZ74_09645"
     CDS             3909..4796
                     /locus_tag="PKZ74_09645"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPM93062.1"
                     /translation="MIHLNPAEIPHTKLHQYLLSGIAPRPIAFASTVDASGRPNLSPF
                     SFFNVFGVNPTTLIFSPSRRSRDGSVKDTLENLKAVPEVVINVVTHDMVNQASLASSE
                     YPKGVDEFVKSGFTKLPSEIIKPFRVKESPMQMECRVREIIETGTGGGAANLVICEIL
                     LIHISEDILGEDGMIDQHKIRLVGRLGKDFYVKAFGDALIEVEKPARNIGIGIDALPE
                     FIRHSAHLTGNDLGKLGNLERMPSLEEIEEMRQSQDITEILETSCDVYGEVLRLAKAW
                     LDSGHPFKALKILMLLEKI"
     gene            complement(4793..6526)
                     /locus_tag="PKZ74_09650"
     CDS             complement(4793..6526)
                     /locus_tag="PKZ74_09650"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF010657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HPM93063.1"
                     /translation="MMEMNEILGKLPHHLMQLVIDQPYNSYTAQDHAVWRYVMRQNVR
                     YLSKVAHGSYLDGLKRTGISIDSIPHMYGMNRILKEIGWAAVAVDGFIPPAAFMEFQA
                     YNVLVIAADIRPIDQIEYTPAPDIIHEAAGHAPIIADPEYAEYLKLFGRIGSRAFASA
                     RDHEIYEAVRHLSILKADPYTNPESILQAGKELAEAEAIHETTSEMAQIRNLHWWTVE
                     YGLIGDLKNPKIYGAGLLSSIGESFSSLQPQVKKLPYSIDAIHYNFDITTRQPQLFVT
                     PDFQFLTTVLNEYAGTMALNTGGIDGVMKAIESGYTGTVVYSSGLQVSGTFQEMIIHR
                     EALVYIRTEGPTQLCADDRQLEGHGKDYHVHGFGSPVGKLNVSTVPLEILSDGDLAAL
                     GIITGEEASLEFESGLMVTGRLERIVRNRDKIVVMSFSGCTVRFRDKVLFDPSWGLYD
                     MAVGEKIVSAYAGPADPFAYRLEYPVPKEKTHKIIHSDRAARLHGLYQAVRDHREGKQ
                     MIPDPVQLFNELKNDYPEEWLLPLEILEIIHGRSGYQLHEEIKSYLNQKGRENEDFGR
                     LIKRGIEEIDS"
     gene            complement(6661..>7203)
                     /locus_tag="PKZ74_09655"
     CDS             complement(6661..>7203)
                     /locus_tag="PKZ74_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93064.1"
                     /translation="DKDGQTDKINLQITTEEPAGGPINLWETRTLGSWNNLTIGSSFG
                     SINGNVYMLNEAFANQALIDFIYWWGASTDATIGAPNDANAALVFNTGAYALNNWTTR
                     NATMFKTTTVNPAAFDAIDDATECIDIATGADQTRIGTLAADQVIAFVTVTGKHGLIK
                     VKSIETGSAGSITIDVKVEQ"
BASE COUNT         1902 a         1747 c         1717 g         1837 t
ORIGIN      
        1 aatcatgaat tatcgaattg agtaagatag ccatttaaat tctgtcttac ttgttgtaaa
       61 tgagccccaa ttcatgaaaa gcgatacagt aacagtaaag ataaaggaag taacccttaa
      121 aacccggaac ccggaaccaa ttttttccta tctttgtacc cattctaaat aagaaagatt
      181 atgcctcatt atcaaagact aggactaatt ccgccgaaac gccatatcca gttccgtaaa
      241 cccgacgggg ctctctattc agagcagctg gtctcaacag aaggcttttc agccgagtat
      301 tccctggttt atcatgtata cccgcccact gttgttacaa aaattgataa gcccttttcg
      361 gtagcccctg agatcgctgt cgataaaaac atgcagcacc gcagcttcca gggatttaaa
      421 gtagcacccc aagataacta tatcaacagc cggataccag tcctggtgaa cgatgactgc
      481 tatatccatc tggcagctcc gaagtcgtgg gccggtcagc aatccacccg gacaatggaa
      541 aatggacaat cgacaatcga caatcttatc ttctttaaaa actccgacgc ccatgagctt
      601 atcttcatcc accagggtga agggacgctg aaaaccatgt atggaagcat ccctttccgt
      661 tatggcgacc atctcatcat tccgagagga gtgatctacc ggctcgattt caattcggaa
      721 gaaaaccgcc tgtttatcgt cgaatcgttc agcccgttcc gttttccaaa gcggtatgtg
      781 aaccgtatgg gtcaactgct cgagcacgcc ccgttctatg agcgtgatat ccgcaaaccg
      841 gaaaatcttg aaacccacga cgcaaaaggc gatttcatcg ttatggtcaa gaaacaggac
      901 ctggtttatc cgtatcatta tgcttcccat ccttttgatg tcatcggatg ggatggatat
      961 cattttccat atgctttgtc tatccacgat tttgaaccca taaccggacg gatccaccag
     1021 ccgccgccgg ttcaccagac ctttgaagcc aacaatttcg tcatgtgcgc tttcgtgcca
     1081 aggctttatg actaccatcc cgaagctatt cccgtgcctt ataatcacag caatgtcgat
     1141 tccgacgagg tgctctatta tgtcgacggg gaattcatga gccgcaagca cgtggagaag
     1201 gggcagatca ccctgcaccc gatcggcatc ccgcacggcc cgcacccggg aactgtcgaa
     1261 aaaagcctcg gacaaaaaga gacgcgcgaa ctggctgtca tggtcgatac gttccgcccc
     1321 ctgaaaatca caaagcaggc gatggaaatt gaagacagcg aatacttccg gagctgggca
     1381 gagtgaaatt atcaaattat caaattatca aactttcaaa tttctaattt caactttcaa
     1441 tgagcacaga ctttctccct ctcaacggca ccgactacgt ggaatactat gtcggaaatg
     1501 ccaaacaggc cgcctattat tatatgaccg ctttcggctt ccagccgctg gcctattccg
     1561 gccttgaaac cggtaacaag gaaaaaacat cctatgtcct ggtacaggat aagatccggt
     1621 ttgtttttac aacctccctg gtccccgatt cggaaatcgg cgaacatgtt aaaaagcatg
     1681 gcgatggggt caaagtggtt gccttgtggg tagatgacgc aaccagtgcg ttcaacgaaa
     1741 cggtgaaacg gggtgcaaaa ccttatcttg atccggtaaa ggagcaagat gcaatgggtc
     1801 atgttgtgcg atcaggcatc catacctatg gagaaacggt tcatatcttc gtcgagcgaa
     1861 aggattaccg gggcgctttc ctcccgggat tctcaaaatg ggaaccggca taccggaccg
     1921 aacccatcgg cctgaagtat gtggatcata tggtcggcaa tctgggctgg ggcgaaatga
     1981 acaaatgggc gaaattctac gaggatgtga tggggtttaa acagcttatc tcattcgacg
     2041 ataaggatat ctctaccgaa tatacggcac tgatgtcgaa ggtgatgtcg aacagcaacg
     2101 gttatatcaa atttccactc aatgaaccgg cagaaggaaa gaaaaagtcg cagatcgaag
     2161 aatatatcga tttctatgga ggagcgggag tgcagcatgt tgccatggca accgataaca
     2221 tcatacatac ggtcagcgaa ttgaaaaagc ggggagtgga atttttatat gtccccggaa
     2281 catattatga agatgttctc gaccgtgtcg gacatatcga tgaagacctg aaacctttga
     2341 aagacctgaa tatcctgatc gacagggacg acgaaggtta cctgcttcag atctttacca
     2401 aaccggttga agaccgcccg acgttgttct atgagattat ccagcgcaaa ggggccaaat
     2461 cattcggtaa aggaaatttc aaagctttgt tcgaatccat cgaaagggaa caggcccgcc
     2521 gcggaaccct ttaagacagg cttgactttt agtgtaattt ggtgttttag tgctttagtg
     2581 gcaaatagat agagatgtat acttcctgga taaatattcc gcctgaaaac gatttctcca
     2641 tattcaatat cccttttgga ataataagct ataaaggata ttctccacgg ccggcaaccc
     2701 ggatagggga tacagtcgtg gacctcgcgg cgctggccgg atacggcttt ttcgacggat
     2761 tggggatcag cgatctctcc gttttcaatc gcccggcatt gaacgatttt attgctcttg
     2821 gcaaaccggt gacccgggcc gttcgtgacc ggttgatttc gctcttccgg tctgataatc
     2881 cggaactgag agaccattcc gaagcccgaa gaacgtcatt gctgaacgcc gttgatgtga
     2941 cgatgcacct gccggtgaaa gtgggaaatt ataccgattt ctatgccagc gaacagcatg
     3001 cttacaatgt cggcgtgatg ttccgcgatc cgaaaaatgc ccttctgccc aactggaagc
     3061 atattccggt aggataccac ggccgggcct cctcgatcgt ggtcagcggt gcatctattc
     3121 accgtccgat gggccagacc aaagccgacg accaggagtt ccccgccttc gggccttcaa
     3181 agcaggtcga cttcgaactc gaaatgggat tcgttatcgg taaaagttca gctttaggtg
     3241 atcgtgttaa gatttctgat gctgaagatt atatattcgg cctggtattg ttcaacgact
     3301 tgtcggcccg tgatattcag aaatgggaat acgttccgct ggggcctttc ctgagtaaga
     3361 atttcggcag cgtgatctca ccatggatcg tgaccacgga agccctggag ccttttcgcg
     3421 ttcccggccc tgttcaggtt ccggaagttt tgccctattt aaaataccag ggaaaccatc
     3481 attttaacat taaactggaa gttatgcttc agggcaaact tctgacagtt accaattcaa
     3541 aacacctata ctggaatttt gcccagatgc tggcgcatca tacggtgaac ggctgtaatg
     3601 ttgaagttgg cgatatatac ggatcaggca cgatcagcgg gccagagcaa ggctcatacg
     3661 gttcgatgct ggaaattgcc tggaaaggga ccaaaccggt tgtgatgcct gatggcacat
     3721 cccgaagctt catccacgac cacgatacga tagtgatcaa agggtttggt gaaaagcagg
     3781 gaatacgggt aggtttcggc gagtgtaaag tcactatcct ccctgcaaaa aaataaacaa
     3841 gtgattactt cccatttccc aatccttagt aattataact ttgaacttta aattcacatc
     3901 acgttcccat gatccatctc aacccggccg aaattcccca tacgaaactt caccaatacc
     3961 ttctgtcagg tatcgccccc aggccgattg cctttgccag tactgtggat gcatcaggcc
     4021 gcccgaacct gtcgcctttc agtttcttca atgttttcgg cgtaaatccc acaacgctta
     4081 ttttttcacc atcccgacga agtcgggacg gttctgtgaa agataccctg gaaaatctga
     4141 aagccgttcc ggaagtcgta attaatgtgg ttacccacga tatggtcaac caggccagcc
     4201 tggcgagttc cgagtatccc aaaggagtcg atgaatttgt caaatccggt ttcacgaaat
     4261 taccatccga aatcataaag ccattccgcg tgaaggagtc gccaatgcag atggaatgcc
     4321 gtgtgaggga gatcattgaa accggaacag ggggcggggc agcaaatctc gttatttgcg
     4381 agatccttct tatccatatc agcgaagata ttttgggaga agatgggatg atcgaccagc
     4441 acaagatcag gctggtgggt aggttaggca aggactttta tgtgaaagct tttggtgatg
     4501 ccctcattga agtggaaaaa ccggcccgta atatcgggat cgggatcgac gccctgcctg
     4561 aatttatccg ccatagcgcc catcttacgg gaaatgacct gggtaaactg ggaaacctgg
     4621 agaggatgcc atctctggaa gagatagaag aaatgcgaca aagccaggat ataactgaaa
     4681 ttctggaaac ttcgtgcgat gtatacggcg aagttttgcg gctggctaaa gcctggctcg
     4741 attcaggaca cccatttaaa gcgcttaaaa tattgatgtt gctggaaaaa atttaagaat
     4801 cgatttcctc aatacctctc tttatcagtc ttccaaaatc ttcattctct cttccctttt
     4861 ggttcagata ggatttgatt tcctcatgta attgataacc tgacctgccg tggataattt
     4921 ccaatatttc caaaggcaga agccattctt ccggataatc gtttttaagt tcattaaaaa
     4981 gctgaaccgg gtcaggtatc atttgttttc cttccctgtg gtccctcaca gcctggtata
     5041 atccgtgaag tcgggccgcc cggtcgctgt gaataatttt atgtgttttt tccttcggaa
     5101 caggatattc gagacggtaa gcaaatggat ctgccgggcc ggcataggcg gaaacgattt
     5161 tttccccgac agccatatca tacaaacccc aggaaggatc aaaaaggact ttatcacgaa
     5221 atctcaccgt acagccggaa aaactcatca caactatttt atccctattt ctgacaatcc
     5281 gttccagtct tccggtaacc atcaggcctg attcgaattc caggcttgct tcttctcctg
     5341 ttattatgcc aagagcggca agatccccat ctgaaagaat ctccagaggt actgtcgaaa
     5401 cattcagctt tccgacaggc gatccgaagc cgtgaacatg gtaatccttt ccatgccctt
     5461 ccagttggcg gtcatcggca caaagctgcg ttggaccttc ggtccggata tagaccagcg
     5521 cttccctgtg tatgatcatc tcctggaagg tccctgagac ctgtaatccg gagctataaa
     5581 caacggtacc tgtataaccc gactcaatgg ctttcatcac accgtcgatt ccgcctgtat
     5641 tcagcgccat cgtgccggca tattcgttca ggactgttgt aaggaattgg aaatccggtg
     5701 taacaaatag ctggggctgg cgggtagtga tatcaaagtt atagtggatg gcatcaatgg
     5761 agtaggggag cttttttacc tggggctgta atgatgaaaa gctttccccg atagaagaga
     5821 gcaagccggc cccgtagatt ttgggatttt tcagatcgcc gatgagtccg tattccactg
     5881 tccaccagtg caggttacgg atttgcgcca tctcggaggt cgtctcatgg atggcttcgg
     5941 cctcagccag ctctttcccg gcctgcagaa tggattcagg attagtataa ggatcggctt
     6001 taagaataga aagatgcctg acggcttcgt aaatctcgtg atccctggca gaagcaaagg
     6061 ctcttgaccc gatccggcca aacaacttca gatattccgc atattctgga tcagcgatga
     6121 tgggtgcgtg tccggctgct tcatgaataa tatcaggggc cggggtgtat tcaatctggt
     6181 cgatcggccg gatatcagct gcaataacaa gcacattgta ggcctggaat tccatgaatg
     6241 cggcaggcgg gatgaaaccg tcgacggcaa ctgccgccca tccgatctct ttcaggatcc
     6301 ggttcatacc gtacatatgc ggaatgctgt cgatggagat gccggtcctt tttaatccgt
     6361 cgaggtagga gccgtgtgcc actttactca gatacctgac gttctgcctc atcacataac
     6421 gccaaacggc atggtcttgc gccgtatagg aattataggg ctgatcgatg accagttgca
     6481 tgaggtgatg cgggagtttg ccaagtattt cattcatttc catcatcttc caaaattacc
     6541 atttttcatg tactttcagg cttctccgaa attgattaac gaaaatttaa ccaaaaaaat
     6601 gttaaaaaaa aagaggctgt ccgatgaacc gggcagcctc ttccggagat ttataatatc
     6661 ctattgctca acctttacat caatggtaat ggatccggca gaaccggttt caatgctctt
     6721 caccttgatc aaaccatgct ttcccgtcac cgtgacgaaa gcaatgacct ggtcggcagc
     6781 aagagtgccg atacgggtct ggtctgcgcc ggtagcgata tcgatgcact cagtcgcatc
     6841 gtcgatggca tcaaaggcag ccggattgac cgtagtggtt ttaaacatgg tcgcattgcg
     6901 tgtggtccag ttattcaggg cataagctcc cgtgttgaat accagggcag cattggcatc
     6961 attaggcgca ccaatggttg catctgttga agcgccccac cagtaaatga aatcgatcag
     7021 tgcctggttg gcgaatgctt cgttcagcat ataaacgttt ccgttgatgg atccgaatga
     7081 actgccgatc gtcagattgt tccatgatcc gagcgtacgg gtctcccaca ggttgatggg
     7141 accaccggcc ggttcttcgg tcgtgatctg aaggttgatc ttgtcagtct gtccgtcctt
     7201 atc
//
LOCUS       DAOPNH010000173         7191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11778_length_7191_cov_4.525645, whole genome shotgun sequence.
ACCESSION   DAOPNH010000173 DAOPNH010000000
VERSION     DAOPNH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11778_length_7191_cov_4.525645"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3897)
                     /locus_tag="PKZ74_09660"
     CDS             complement(<1..3897)
                     /locus_tag="PKZ74_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93065.1"
                     /translation="MKRILFSGLISIFLCSVAIAQVSINPNGSPPHPSAGLEISFPNK
                     GFLLPRLTFEQMNNIPEPAEGLLIYCVDCNTDGSGSLAIFQNQAWRAFNLNCAKPNTP
                     TSGIKVPATTSIEWKWNPVPIAEGYKWGLTPDYESALDLGDTLAHLESGLSCWTEYIR
                     YVWAYNECGASQPLVLTGSTTDIPFTPAPTPFLILPTESTILWSWQAVPDAVGYRTIS
                     GNNPDFYSAQDIGDSILFLDEGLDCLTEYKRYVWAYDECGFSSAVMMTAWTTADPPPS
                     PAAGVHTATSTEIIWDWNSVTGADSYLWNDIDDTTGSVKTGTTTSYSESNLNCLTNYT
                     RYVWSLSECGLSDPTVLQHSTLSNPPTAPAPAAHVPSAYQVVWNWNVVEGVDGYRWGT
                     SNDYNEAEPMGTLTSKTETGLNCNTDYTRYVWAYDECGVSGVTILTQKTSLDPPDEPV
                     EAAHTPSPNQIIWNWNPVAGATGYRWGVTNVIESSVDVGNVTSRTETGLNCNSSYSRY
                     VWAYSSCGTSGATTLTQVTSLDPPDAPVAATHNPGPTQITWNWNAVAGATGYRWSQNN
                     DYENSTPLGNNTSTTQSGLTCNTPYVSYVWAYSSCGVSEATQLSQTTALDPPAAPVPS
                     VHVATANQITWNWNSVPTATYYLFNDENDPGTATNLGNVTTYIESNLTCFTPYTRFIW
                     AASNCGISLVTSLSKTTDLDPPDAPVAASHIPSYNQIVWNWNSVPDATGYKWWPTNDY
                     NAAQDMGSSTTKTQTSLECTTEYTSYVWAYSSCGHSVVTLLSEETTENPPAPPTPGTH
                     NVTATSITWNWNAVAGADGYKWNTSNNYQAAFDLGTIRTVTQNDLTCNTPYSIFVWAY
                     SVNCGVSTPVTLNATTAMNPPAIPTAGTHVPAVNAITWNWNPVAGATGYKFSATNNYA
                     TASDLGNVTTWNESLPCNASYVRFVWAYNGNCGNSGSRTLSASTLADPPELPVAAVHV
                     AEAYQITWKWHPVQDATGYRWNSVNDLASATPPSTDTSSTQGNLTCLSNYNSYVWAVG
                     PCGTTTAVTLTQMTGQDPPDAPSAATHIASANGIEWHFNTVPEATGYRWDFDNNPEGS
                     FDIGTNTTWTETNLACNTLYTRYVFSVGPCGTSAASTFTQSTLNEPPATPVSGSHVPA
                     ITSIEWKWNPVAGAVGYLWNTENTTVGATDKFNVTSHTEINLGCNLPQTAYVWAYNGC
                     GNSTPVTLTSATLLDPPAAPAEGTHIADVQEIEWHWNAVSGATGYLWNTVNDTTNSVD
                     VGNLTTYTESGLTCNSAYTRYVWAYSNCGLSGSTTLT"
     gene            complement(4329..4844)
                     /locus_tag="PKZ74_09665"
     CDS             complement(4329..4844)
                     /locus_tag="PKZ74_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM93066.1"
                     /translation="MGWRITGVIPPGTRKCVIAVAPHTSYWDFVIGRLAYWVMGIKAS
                     FLIKKEVFRFPFKFLLLSMGGIPVDRGRSSKMVEQVAEQFRKADSLYIVITPEGTRKP
                     VRNWKKGFYYIAMQAQVPIALGYLDYARKEGGVGKVIFPNGDYDSQLKEIKEFYRSMT
                     PKHPEKFILGD"
     gene            4971..>7191
                     /locus_tag="PKZ74_09670"
     CDS             4971..>7191
                     /locus_tag="PKZ74_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM93067.1"
                     /translation="MPIKLRYALVKVPFAYLARYIILPCCLAALLSSCSNTRLLSEGE
                     QLLIDNRIHFQDKNPGIPSDELLSIARPRPNHKFLGMARVKLYFYKLGTHGKPESKFR
                     TWLREKAGERPSVFDSASSLQSIAEMKQYLNKTGYFFSSVEFRTNHSRKKKVVVDYLI
                     TPSVPYRVKSVRYDIHDSIMLSFIEKSIADSRIITGDKYNAFNLDDERDRMTEILQNN
                     GYFYFTRDYIYFEIDSAFRSNELEINVKVKPWMDDTLKGIAQFHKRYFIRNVFITPGF
                     NPMAPVTAKQDTIGLEIPGALRKKYSGDYFLIQEKDDHIHPRTIIQSVFIRPGDPFRQ
                     VNIGRTRSRLSEIGVFGYTNIRFKPVTGLPDTITDGILDCQIDLTKRKPHSFTVETEV
                     TNSGGRPGIGLNFTYTNSIIFRGSETFRMKARGALEAQKIFGSNAEYLDGTPFFNTIE
                     SGLEMGLLFPRFLIPVRQERFPRYFRPKTTISLGIGYEKRPEFERWLTNLTFGYDWKE
                     SERKRHQIFPFDWSLVNVNLSPDFKQVLEDEPNDRIKNQYTDNFITAIRYSYTYNTQD
                     IKKIRDFFFFKGNLETAGNILNLGSNLLRAPVDSLGRYILFGIPFAQYVKIDGDFRFF
                     NVITRNTSFAYRLFLGAGWPYGNARFLPLEKGYYAGGANGMRGWPYRLLGPGSYNNPD
                     DNFDRMGDIQLEANLELRFPIYSFIKSAIFADIGNIWLMKEQESYAGGNFSFSRFYKE
                     "
BASE COUNT         1887 a         1669 c         1760 g         1875 t
ORIGIN      
        1 ggtaagcgtt gttgatcctg ataatccgca attactatac gcccatacat aacgtgtata
       61 agctgaatta catgtaagac cggattcggt ataggttgtc aagttaccga catccacaga
      121 gtttgttgtg tcgtttacag tattccacag gtagcccgtt gcaccagaaa ccgcgttcca
      181 atgccattcg atctcctgaa cgtcggcaat atgggttccc tctgccggtg ctgccggagg
      241 atccagcaat gtggctgaag tcagggtaac aggagtggaa ttgccgcatc cgttataagc
      301 ccagacatag gcagtctgtg gcaaattaca gcccaggttg atttccgtat gggatgttac
      361 attgaactta tcggtggccc ccactgttgt attttccgta ttccacagat atccgactgc
      421 acctgccacc ggattccatt tccactcgat cgacgtaatg gccggtacgt gtgacccgga
      481 aaccggggtt gcaggaggct cgtttaaagt ggattgtgta aaagtagaag cggcagaggt
      541 tccgcaaggc ccgactgaaa atacataccg tgtataaaga gtattgcaag ccagattcgt
      601 ttcggtccag gttgtattcg ttccgatatc aaatgaacct tccggattat tgtcaaaatc
      661 ccaccggtat cctgttgctt ccggaactgt attgaaatgc cattcaattc cgttagcaga
      721 tgcaatgtgt gttgcagcag aaggggcatc cggcggatcc tggccggtca tttgtgtcag
      781 tgtcacggcg gtggttgttc cgcacggacc gactgcccag acataggaat tataattaga
      841 caggcaggtt aaattacctt gagttgagct tgtatccgtt gatgggggtg ttgcagaggc
      901 cagatcattc acggaattcc acctgtaacc tgttgcgtcc tgaaccggat gccatttcca
      961 ggttatctgg tacgcttccg ctacatggac cgctgcaaca ggtaattccg gcggatccgc
     1021 cagggttgag gcagaaagag tccgggaacc tgaatttccg caattgccat tataggccca
     1081 tacaaatcgc acataacttg cattgcaagg caggctctca ttccaggttg tcacattccc
     1141 taaatcactg gctgtggcat agttatttgt agcgctgaac ttatagcctg tggcaccggc
     1201 aacaggattc caattccagg taatggcgtt tacagccggg acatgtgtac cagcggtagg
     1261 aattgccggc gggttcattg ctgtggtggc attcagggta accggggtgg agactccgca
     1321 attaacgcta taagcccaga cgaagattga ataaggtgta ttacaggtca gatcgttttg
     1381 tgttacggtt ctgattgtgc ccaggtcaaa tgcagcctgg taattattgc tggtattcca
     1441 tttataccca tcggcgccgg ctacagcatt ccagttccat gtaatggatg ttgccgtaac
     1501 attgtgagtc cctggagtcg gtggagcggg cgggttttcg gttgtctcct ctgaaagcaa
     1561 tgtaactact gaatggccac aactgctgta tgcccagaca taactagtat attcggtggt
     1621 acattccagg ctggtctggg ttttcgtggt ggatgatccc atatcctgag ccgcattata
     1681 atcatttgtc ggccaccatt tatatcccgt agcatccggg accgagttcc agttccaaac
     1741 gatctggttg tatgagggga tatgactggc tgcaacaggt gcgtcaggcg ggtcaaggtc
     1801 ggtcgttttg ctcaggcttg taaccagaga gattccgcag ttgctggctg cccagataaa
     1861 gcgggtatat ggggtgaagc aggttaagtt ggactcgata taagtcgtca cattccccag
     1921 gttagtcgcc gttcctggat cattttcatc gttgaataaa taataggtag ctgtcggtac
     1981 gctgttccag ttccatgtaa tctgattggc cgttgcgaca tgaacggatg gaacaggtgc
     2041 tgccggcgga tccagcgcgg tagtctggct gagctgagtc gcctcagaaa caccgcagga
     2101 actatatgcc caaacatagc ttacataagg tgtattgcat gtaagtccgg attgtgtagt
     2161 ggatgtattg ttgccaaggg gggtggaatt ttcataatcg ttattctggc tccagcgata
     2221 accggtagcg ccggcaacag cattccagtt ccaggtaatc tgcgtcggtc cgggattatg
     2281 agtggctgct accggtgcgt cgggaggatc cagagaagta acctgggtga gggtggttgc
     2341 ccccgaagtt ccacaactac tgtaagccca gacgtaacgg ctgtatgaag aattacaatt
     2401 taaaccggtt tcggttctgg aagtaacatt tcccacatca acagaggatt ctatgacgtt
     2461 agtcactccc catctgtatc ctgtggcacc ggccaccggg ttccagttcc agatgatctg
     2521 gttcggggaa ggtgtatgcg ctgcttcaac gggttcatcc ggtggatcaa gggatgtctt
     2581 ttgtgtgaga atcgtcacac ccgaaacgcc gcattcgtca taggcccata catagcgcgt
     2641 gtaatcggta ttgcaattca accctgtttc ggtttttgag gtcagggtac ccatgggttc
     2701 agcctcatta taatcgttcg aagtacccca gcgataacca tcaacacctt ccacgacatt
     2761 ccagttccat accacctggt aagccgatgg cacatgagca gccggtgcag gagcagttgg
     2821 agggttactg agtgtggagt gctgcagaac cgtcggatca gacaaaccgc attcgcttag
     2881 tgaccaaaca taccttgtat aatttgtcag gcagttcagg tttgattcgg aataactcgt
     2941 tgttgtgccg gtttttactg atccggtcgt atcatctata tcattccaca ggtatgaatc
     3001 ggcaccggtt acgctgttcc agtcccagat tatttccgtt gaagttgccg tatgtactcc
     3061 tgcagcaggt gacgggggcg gatcggcggt tgtccaggca gtcatcataa cagctgagga
     3121 aaatccgcat tcatcatacg cccaaacata tcgtttatat tctgtcaggc agtccagacc
     3181 ttcatccagg aacagaatcg aatcaccgat atcctgtgcc gaatagaaat cagggttatt
     3241 accggaaata gtgcggtaac cgacggcgtc aggaacggcc tgccagctcc atagaatagt
     3301 tgattcggta ggcaggatca gaaatggggt tggcgccgga gtgaacggaa tatcggttgt
     3361 actgccggtg agaacgaggg gttgcgatgc gccgcattca ttgtaggccc aaacatacct
     3421 gatgtactcg gtccagcatg aaaggcctga ttccaggtga gcaagtgtat cgcccaggtc
     3481 aagtgccgat tcataatcag gggtcaaacc ccacttataa ccttcagcaa tcggaaccgg
     3541 attccatttc cattcgatag aggtagtagc cggaacctta atgccggaag tcggcgtatt
     3601 gggtttggca caattcaggt taaaagcccg ccaggcctgg ttctggaata tcgccagcga
     3661 tcctgatcca tccgtattgc aatcaacaca atagatcagc aatccttcag ccggttcagg
     3721 aatattgttc atctgctcga atgtcagcct gggcagtaaa aaccctttgt tggggaatga
     3781 aatttccaat cccgccgaag gatgcggcgg gctaccgtta gggtttattg atacctgagc
     3841 aatggcgaca ctacagagaa aaatggatat caggcctgag aatagaattc ttttcatgat
     3901 gttatattta gattaaagga ctaagaaaca tatcaactta acaaagctat ttctgaaaaa
     3961 ccagcaatta ataaatgcaa ttgattattt atcaacccat tattgttagt atctgatttc
     4021 cgctgaggaa aagacccgga aaagaagtat aaatagaacc tttcagagtg atcagggtat
     4081 ataaatgaag tttcaatcgc attcatgcaa ctttatcgaa aaaaaccaaa aaagtaatat
     4141 caaaatctgg aaagtgtcgt tattggtgat ttccaatatg ttcaaccgga aagggatcgt
     4201 tttaaaggcc tggatggtgg ctcgaaagca atctgagttc cggacctccg attgatattc
     4261 cgttgacaaa agtaatagag aaatcctaaa accggagatt aaaatgaaat ttagccgatt
     4321 attgttttct aatctccgag gataaatttt tccgggtgct tcggggtcat gctccggtaa
     4381 aattctttta tttctttcaa ctgactgtca taatccccgt tgggaaagat aaccttaccc
     4441 actccccctt ctttccgggc atagtcgaga tagcctaagg caatgggcac ctgtgcctgc
     4501 atggcaatat agtagaatcc ctttttccag ttcctgacgg gtttgcgtgt tccctcagga
     4561 gtgatcacga tatacagcga atcggctttc ctgaattgct ccgctacctg ttccaccatc
     4621 ttgctgcttc ttcccctgtc gaccggaatt ccacccatgc ttaagagcag gaacttgaac
     4681 ggaaaccgga aaacctcctt ctttattaaa aaactggctt tgattcccat gacccagtat
     4741 gcgagccggc caatgacaaa gtcccagtag ctggtgtggg gagccacagc aataacacat
     4801 ttccgggtgc ccggaggaat aacgccggta atcctccatc ccataatttt aagtactgct
     4861 tttgcgaaca atgctaacat aataatggca ttcgtttgca aaattaaaaa tttaccggaa
     4921 ggcctgacgg taattaatgg ccgttctgtt tttcacaaaa atccatacct ttgcccatta
     4981 agctgcggta cgctttggtg aaggttccat tcgcatattt agcccgatat ataattctgc
     5041 cctgctgcct tgcagcactt ttatcctcat gctcaaatac gcgactgtta agcgagggag
     5101 agcaattgct gatcgataac aggatccatt tccaggacaa gaatcccggt attccatccg
     5161 atgaattgct ttcgatcgcc cgaccccgtc caaaccataa attcctcggc atggccaggg
     5221 tgaaactata cttttacaag ctggggaccc atggcaaacc cgaaagcaaa ttcaggacct
     5281 ggctcaggga aaaagccgga gaacgcccct cggttttcga ttcggcttca tcgctgcagt
     5341 ctattgccga gatgaagcaa tacctcaata aaacaggcta tttcttctcc tccgttgaat
     5401 tcagaacaaa tcattcaagg aagaaaaagg ttgtcgtaga ttacctgatc actccgtcag
     5461 ttccataccg ggtaaagtcg gtgagatatg acatccatga cagtataatg ctttcattca
     5521 tagaaaaatc aatagccgat agcagaataa taacagggga taaatataat gcctttaacc
     5581 tggatgatga acgggacagg atgaccgaga tcctgcagaa caacggctat ttttacttta
     5641 cacgggatta tatttacttt gagatcgaca gtgcatttcg gagcaatgaa ctggaaatta
     5701 acgtaaaggt caaaccctgg atggatgata cgctgaaggg tattgcacag ttccataaaa
     5761 ggtatttcat acgcaacgtg ttcatcaccc ccggtttcaa tccaatggcc ccggtcacag
     5821 caaaacagga tactatcggc cttgagatcc cgggggcgtt aagaaagaaa tacagcggtg
     5881 actatttcct catccaggag aaagatgatc acattcatcc acgtaccatt attcagtcgg
     5941 tttttatccg gccgggagat cctttcaggc aggtgaacat cggaagaacc cgctccaggt
     6001 tgtcagagat cggtgttttc gggtatacca atatcaggtt caaacctgtc acaggattac
     6061 cggacacgat cacagacggg attctggatt gccagataga ccttacaaaa cgaaagccgc
     6121 actctttcac cgttgagact gaagtgacca attcgggagg aaggccgggg atcgggctta
     6181 atttcaccta taccaacagt attattttca ggggatcgga aacattcagg atgaaagcac
     6241 gcggagcgct cgaagcgcaa aaaatattcg gcagtaatgc agaatacctg gatggcaccc
     6301 cattcttcaa tactattgag tcaggcctgg aaatgggcct tttatttccc cggttcctta
     6361 tccctgtgag gcaggagcgt tttcccaggt atttcagacc caaaacaacc atcagcctgg
     6421 gtatcggtta tgaaaaacgg cccgagtttg aacgatggct gaccaaccta acttttggtt
     6481 atgactggaa agagtcggaa cggaagcggc accaaatatt cccgttcgac tggagcctgg
     6541 tgaatgtgaa cctctcaccc gatttcaagc aagtcttaga ggatgaaccc aacgacagga
     6601 tcaaaaatca gtatacggat aactttatca cagccatccg gtactcttac acttacaaca
     6661 cacaggatat taaaaagata cgtgatttct ttttctttaa aggaaacctg gaaactgccg
     6721 gcaacatact caatctggga agtaacctcc tccgggcacc ggttgattct ttaggcaggt
     6781 acattttatt tggtatcccc tttgcccagt atgtgaagat cgatggcgat ttcaggttct
     6841 ttaatgttat tacccggaac acttcgtttg cctacaggct gtttctcggt gcaggatggc
     6901 catatggcaa tgcccgcttt ctaccgcttg agaaagggta ctatgccggc ggagcgaatg
     6961 ggatgagggg ctggccgtac cgtttgctcg gtccgggctc atacaataat cccgacgaca
     7021 acttcgaccg gatgggggat attcagcttg aagccaacct ggaactgcgt ttccctatat
     7081 acagtttcat taaaagcgcc atttttgcag acattggcaa catatggctg atgaaggaac
     7141 aggagtcata tgccggcggc aatttcagtt tcagccggtt ttacaaggag t
//
LOCUS       DAOPNH010000174         7161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11841_length_7161_cov_4.259499, whole genome shotgun sequence.
ACCESSION   DAOPNH010000174 DAOPNH010000000
VERSION     DAOPNH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11841_length_7161_cov_4.259499"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PKZ74_09675"
     CDS             complement(<1..1662)
                     /locus_tag="PKZ74_09675"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPM93068.1"
                     /translation="MVFDLNMIKGVYAGLAAKVQKARQLTGKPLTLTEKILYSHLSAE
                     NDPAPYYRGKSYVNFDPDRVAMQDATAQMALLQFMQAGKKRVAVPSTVHCDHLIQAKA
                     GAREDLMTALDINREVFDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGMPWELKFPKLIGVKLTGKLNGWTSPKDVILK
                     VAGILTVKGGTGAILEYFGPGALDISCTGKGTICNMGAEIGATTSVFGYDRKMEEYLR
                     GTGRAEVADSANVVAEHLTADPEVYMNPEKYFDQVIEINLTELEPHINGPFTPDLATP
                     VSKFAREVEKNGWPEKLEVGLIGSCTNSSYEDITRAASVARQAIDKKLRAKSEFTITP
                     GSEQVRFTAERDGYLDIFEKIGGVVLANACGPCIGQWARHNAHKQEKNSIMTSFNRNF
                     AKRNDGNPNTHGFVASPEIVTAFAIAGTLKFNPVTDKLLNEEGKEVSLDEPVGIDFPV
                     NGFAVEDNGYQPPAEDGSAVNVIVDPKSDRLQLLAPFPAWNGEDLT"
     gene            complement(1829..5011)
                     /locus_tag="PKZ74_09680"
     CDS             complement(1829..5011)
                     /locus_tag="PKZ74_09680"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPM93069.1"
                     /translation="MKRSVLVFLFFSLLGLYGYNQVFDERKNPKEMTPEEQQYLLERD
                     RPAKEFPVSPEGGTRAVGDDCTNPIIISIPAALPYNVTGQTNCGRGNTYSTSCLGNYD
                     GGEDIMYRLDVTATTLVTITMNPGTTTYTGMYLSATCGNSGTCIATSTGSGATPRVIS
                     NQTLTAGNSYYVMVDTWPTPNCIPSFSLNIVAVVANPVSFSASAFSSSQINLTFTPNA
                     SNHNVVIVWNNTGTFTTPAGTPPAAGQSFAGGTLLYNGTTSPQSHTGLNGGTIYYYKA
                     FSYDGSGYSSGLTANATTPCAAYSIPYTQDFNGSYLPTCWYEANGNAPNPTLSSSSWT
                     NSTYFANTTGNSAAVKMNLYGTDNDWLISPPIDLGSGSRRITLKVAGTTYNGTGSVTM
                     SADDTVFVLYSTNLTAPYNFVKANAITYYTNTHSPSVAGWDEVYELNGLSGNVLFAFY
                     AKGINNTPDMDFHIDDFVVEEVPACPKPTGLFTDNIGFTQADLDWVQSGSPAGWEIIW
                     GLPGFDPETEGTLITGVSSHPYTLNPPLDHSTIYQWYVKSDCGDGMLSGWSGPASFQT
                     LCQDITVYPYSQSFDGIAFPPICWDTSLIVTGTGSGTSGNWDRVTSGTFPTCSPFSGV
                     GMTKFASFNFKSGTKSIMVSPSLELPKDYYSVSFWMFRDNGYPTNNDMINIYYNNSPD
                     LNGAELLGSVSRYYLSSPVEATANLWYNYEFTLPEGSAGSGRYIIVEGVSAYGNNIFF
                     DEFIIREPSSLSGYVLDFDGNPVEGVLVEKVGGINTYSAADGSYELYPLPSGNQQFKC
                     SMTGNNDLFEYVDIPLYTTVNHNFTMLAPEINLSPGSFLFILGESETGSENLILTNTG
                     NGTLDWVLEELDGAPVDVPWLVADDYAGQLNPFGDSQTLLVTVDASLTPGSKAPGTTY
                     QAGLKFSSPSGIEPDTVPITLIVTDGGVKGPENLQVYVVSSNDGKFMLKWLYFTIKGL
                     NFEHFLILQNGQVIGTSEGSSFNVELDTPGMYCYKVFAVYSNGVYSNASNVACINYPL
                     PPNVPVSNWALLLAAGLMIIITIVYFRRRF"
     gene            5406..6689
                     /locus_tag="PKZ74_09685"
                     /pseudo
     CDS             5406..6689
                     /locus_tag="PKZ74_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(6725..>7161)
                     /locus_tag="PKZ74_09690"
     CDS             complement(6725..>7161)
                     /locus_tag="PKZ74_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPM93070.1"
                     /translation="ILKNLHTLHIRMRKHSENALYLSDRLEKDGLRIIYPGLSSHKDH
                     DLMKSLMNPDFGFGGMMAIDMGTADKAYAFMERMQNEGVGYLAVSLGYFKTLFSNPGK
                     STSSEVPEHIQREMGLSEGLVRFSVGLDHDIERTYQRIKKCL"
BASE COUNT         1833 a         1681 c         1722 g         1925 t
ORIGIN      
        1 tgtcaggtcc tcaccattcc aggcagggaa cggagcgagt aactgaagcc ggtcggattt
       61 gggatcgacg atcacattca cagcgcttcc gtcttctgcc ggaggctgat agccgttatc
      121 ttccaccgca aaaccgttga ccggaaagtc gataccgacg ggctcatcca ggctgacctc
      181 ttttccttct tcattgagga gtttatcggt aacaggattg aactttaagg ttccggcaat
      241 ggcgaatgcg gtgacgattt cgggtgatgc aacaaagccg tgcgtattgg gatttccgtc
      301 gttgcgtttg gcgaaattcc ggttaaaaga ggtcatgatg gaattttttt cctgcttatg
      361 ggcattatgg cgcgcccatt gaccaatgca gggcccgcag gcattggcca gcactacccc
      421 tcctattttc tcaaagatat cgagataacc gtctctttca gcagtaaaac ggacttgttc
      481 ggaacccggc gtaatagtga attccgattt tgccctgagc tttttatcga tagcctgccg
      541 ggcaacggag gctgcccgcg taatgtcctc gtacgacgaa ttggtgcagg aaccgatcag
      601 gcctacctcc agtttttccg gccagccgtt cttttccact tcccgggcaa attttgagac
      661 gggagtggct aaatcgggcg taaaaggtcc attgatgtga ggttccagtt cggtcagatt
      721 tatttcgatg acctggtcga aatacttttc agggttcata taaacttccg gatctgcggt
      781 gagatgctcc gccacgacat tggcgctgtc ggccacttca gccctgccgg ttccccgtag
      841 gtactcttcc attttgcggt cgtagccaaa gacagaggta gtagccccta tctctgctcc
      901 catattgcag atggttcctt tgcctgtgca ggagatatcc aaggcgcccg ggccgaagta
      961 ttccaggata gccccggttc cgccttttac ggtgaggatc cctgcgactt tcaggatgac
     1021 atcttttggg gaggtccagc cattcagttt gccggtgagc tttaccccga taagtttagg
     1081 gaatttcagt tcccagggca tgccggccat gacgtcgacc gcgtcggcgc caccaacacc
     1141 tatggctacc atacccagac cgcctgcatt gaccgtgtga ctgtcggttc cgatcatcat
     1201 ccctccggga aaggcatagt tttccagaac cacctggtgt atgatgcccg caccgggttt
     1261 ccagaaaccg ataccgtatt tattgctaac ggatgacaga aagtcaaaca cttcccgatt
     1321 aatatccagg gcagtcatca gatcttccct tgcgccggct ttagcctgaa tgagatggtc
     1381 gcaatggacg gtactgggaa cagcaaccct cttttttccg gcttgcataa actggagcag
     1441 ggccatctgg gcggttgcat cctgcatagc caccctgtca ggatcaaaat tgacatacga
     1501 cttgcccctg taataaggtg ccggatcatt ttctgccgac agatgggaat acaggatctt
     1561 ttccgttaat gttagcggtt tgccggtcaa ttggcgggct ttttgaactt ttgcagccag
     1621 gccggcgtaa acgcctttta tcatatttaa gtcgaatacc atagaaatgc taaatattga
     1681 atattgaaaa attgaatttt aaatatttaa ttgattgtaa aaggagtgca aaaatagtca
     1741 atttagagaa aagaaaagcc ctgcagaatt tgaatcgctg cagggctgaa aatcacttaa
     1801 tcccggcaat taacctaaga attgtttttt agaatcttct tctgaaatag actattgtta
     1861 tgatgatcat aagtcctgct gccagcagaa gcgcccagtt cgacaccggc acattcggtg
     1921 gcaaaggata attgatgcat gccacatttg aggcatttga ataaacacca ttggaataaa
     1981 cggcaaatac tttatagcaa tacataccgg gagtatccag ttccacattg aatgaactcc
     2041 cttcagatgt accgataacc tggccattct gcaaaataag gaagtgttcg aaatttagtc
     2101 ctttgatggt gaaatacaac cacttcaaca taaattttcc atcattggat gaaacgacat
     2161 agacctgaag gttttcagga cctttaacgc cgccatcggt aacgatcaaa gtgatgggta
     2221 ccgtatccgg ttcaatgcct gagggtgatg agaatttcaa tccggcctgg taagttgttc
     2281 caggggcttt tgatcccggc gtgagcgatg cgtcaacagt taccagcaga gtctgactgt
     2341 caccaaaagg atttaactgg ccggcataat catcggcgac aagccaggga acatccaccg
     2401 gggcaccgtc gagttcctcg aggacccaat ccagggttcc gttgcctgta ttggtcagga
     2461 tcagattttc gcttcctgtt tcactttctc caaggatgaa caggaaggag cccggtgaca
     2521 gattaatttc cggagccagc atcgtaaaat tgtgatttac tgtcgtataa agcggtatgt
     2581 cgacatattc gaagaggtca ttgttgccgg tcatggaaca cttgaactgc tggttaccgg
     2641 atggcagcgg atataattcg taggatccgt cggcagcaga ataggtattt attccaccga
     2701 ctttttccac taaaactcct tcaaccggat ttccgtcgaa atcaagcaca tacccgctta
     2761 aagatgaagg ttccctgata ataaattcat cgaagaatat attattccca taagcgctga
     2821 ccccttctac aattatatac ctccctgatc cagccgatcc ttccggaagg gtaaattcat
     2881 agttatacca aaggttggct gtggcctcaa caggtgatga caggtaatat cgactgaccg
     2941 atcctaataa ttctgctcca ttcagatcag gtgaattatt ataataaata ttgatcatgt
     3001 cattatttgt aggataccca ttgtccctga acatccagaa agagacactg taataatctt
     3061 tgggaagttc gagcgacgga gaaaccatga tagatttagt accgctttta aagttaaaac
     3121 tggcgaattt agtcattccg actccgctga agggggagca agtcgggaaa gtgccggagg
     3181 tcacccggtc ccagttcccg gaagttccac tgccggtgcc tgtcacaatg agggaagtat
     3241 cccagcagat tggggggaat gcaataccat caaaactttg ggaataagga tatacggtaa
     3301 tatcctggca gagcgtctgg aaagatgcag ggcccgacca accgctcagc atcccgtctc
     3361 cgcaatccga cttcacatac cattggtaaa tagtcgaatg atcgagcgga ggatttaatg
     3421 tatagggatg tgatgagacg ccggttatca gggttccttc ggtttcaggg tcaaatcccg
     3481 gaaggcccca aataatctcc catcccgccg gagagccgga ttgaacccaa tccaggtcag
     3541 cctgagtgaa tccaatattg tctgtaaaca atcctgttgg tttgggacaa gccggcacct
     3601 cttccaccac gaaatcatcg atatggaaat ccatatcggg agtattgttg attccttttg
     3661 cataaaaggc gaataaaaca ttgccgctca gtccgttcag ttcataaact tcatcccatc
     3721 ctgctactga tggtgaatgg gtattggtat aatatgttat ggcatttgct ttaacaaaat
     3781 tatatggagc tgtaagattc gtggaataga gtacaaaaac tgtatcatcg gccgacattg
     3841 ttacggatcc tgtgccatta tatgttgtac cggcaacctt taaagtgatt cgtctgctac
     3901 cggaacccag gtcaatcggc ggcgaaataa gccagtcgtt gtccgttcca tagaggttca
     3961 ttttcacagc tgctgaattt cctgttgtat tggcaaagta agtggagtta gtccaagatg
     4021 aactgctaag ggttggattt ggcgcatttc cgtttgcttc gtaccagcag gtaggtaaat
     4081 aactgccatt aaagtcctgg gtataaggaa ttgaatatgc tgcgcagggc gttgttgcat
     4141 tagctgtcaa accgcttgaa tagccagagc catcatatga aaaagccttg tagtaataga
     4201 tggttccacc gtttaatccg gtatggcttt gaggggatgt ggtcccgtta tatagcagtg
     4261 tcccgcctgc aaagctctgg ccagccgccg gaggagttcc ggcaggcgtt gtaaaagtgc
     4321 ccgtattatt ccacacgatc acgacattgt gattggaagc attgggtgta aaagtgagat
     4381 tgatctggga actgctgaat gcgctcgcgc tgaatgaaac cggattggca accactgcca
     4441 caatattcaa tgagaatgaa gggatacagt tgggggtcgg ccaggtatcc accataacat
     4501 aataggagtt tccggcagtg agcgtttgat tggaaataac ccgtggggtg gcgccgctac
     4561 cggttgaggt ggcgatacag gtacctgaat taccgcatgt tgccgacaaa tacatccctg
     4621 tataggtcgt tgtacccggg ttcattgtga tggtaactaa agtagtggcc gtaacatcca
     4681 ggcggtacat gatatcttca cctccgtcat aattgcccag gcaggatgtg ctgtatgtat
     4741 tcccgcggcc gcaattagtt tgcccggtaa cattataagg taaggctgcc ggaatactga
     4801 taataatcgg gttggtgcaa tcatcgccga ccgctctggt gccgccttcc ggcgatacgg
     4861 gaaactcctt tgcgggccgg tctctttcaa ggagatactg ctgttcttcc ggggtcatct
     4921 cctttggatt ctttctttca tcaaacacct ggttgtaacc gtataaacca agcaaactga
     4981 agaataaaaa aactaaaact gatcttttca taacatttag gtttaatcta atagtaagag
     5041 gaatgattga ttgacgaata tccgaaaata tgaatttact tgttttaatg atttggctat
     5101 aatggtaaaa atcatgaata atatgatctg taccacttgt caaatataag atattctatt
     5161 gtataataca tctgcttatc ctgttctatg aaatttaaac tcaatcttaa taaatggctg
     5221 gaataattag gttcaatttt tctcagaatg taagctaagg tttatctttg gcccataaaa
     5281 tggtacgtcc agtcaaatat aaattcaggg aattcctgct ggatataaaa gaagccataa
     5341 ccggtaccga acgcgatttt accagcgaga aacttggccg ggcgattttt cttttatctg
     5401 ttcctatggt tttggagatg gccatggaat cggtatttgc cattgtggat atctattttg
     5461 tttccaggct cggagctgaa acggtagcaa tagtcgggat taccgagtcg atcatgacca
     5521 tcgtttatgc gttgggcgtt ggtctgggca cagcggcaac ggcaatcatt tcccgaagga
     5581 tcggggaaaa agaccctgaa catgcgtcgc aggctgccgg acaggccata tttgtgggcg
     5641 tatttatttc gttgatgctt gctgttccgt ccgtgatctt ttaccggaac atactccata
     5701 tgatgggaat ggatgagcag gctatcaacg aatacgggag ctacacattg atcatcctgg
     5761 gcacaaatat ggtgatcatg ctcctgttta ttaataatgc catattcaga agctcgggtg
     5821 atccggccat ttctatgaag gtgctggccg tagccaacgg gatcaatata atcctggatc
     5881 ccattctgat attcggattc ggacccatcc cggctttggg aatcaaagga gccgccattg
     5941 caaccacaat cggaagggga ttaggcatcc tgtaccagtt ttacatcctt tttcacggaa
     6001 aacaccggat cggcctgaaa gtccaacatc ttctccccga cctcaaagtg atcctgaacc
     6061 tgttaaagct ggcttcaggg gctgccggac agcatctgat cgctacctcc agctggatat
     6121 tccttgtcag ggtcattgcc ctgtttggaa gcccggtagt agccggttac actattgcca
     6181 tccggattat gttcttcacg ctgttgccct ccctgggcgt cagcaatgcc acggctacgc
     6241 ttgtaggaca gaaccttggc gctgaaaaac cggaacgggc ggggcgggcc gtatagatca
     6301 cagtttatgt gaacattgtc ctgctgggtc tcatcgggct tttcctggcc ctttaccctg
     6361 ccccgcttat ccgtttgttc atcgacgatc cgcaggtgat tgcctacggg acatcctgcc
     6421 tgcgaattct cagtatgggt tatatcgcat acggactcgg aatggtcatg gtcaacgcgc
     6481 tgaatggcgc cggtgatacc cagaccccaa catggatcaa tctgttctgc tactggctat
     6541 tcgaaatccc gctggcctat ttcctcgccg tcaatgcagg gatggggcaa aacggcgttt
     6601 ttatagccat cctcgctgcc gagattgcaa tgacagccag tgcactgtat tttttcagga
     6661 aaggcaaatg gaagctccgg aaagtttagc attccgggat aaaatgatac ttattgagat
     6721 acgctcataa gcacttctta atacgctgat aagtccgttc gatatcgtga tcgaggccca
     6781 ccgagaacct gaccaatcct tccgaaagcc ccatctcgcg ctggatatgc tccggaactt
     6841 ccgaggaggt cgatttcccg ggattgctga acaaagtctt gaaatacccc aggctaacag
     6901 caagataacc aactccttca ttctgcattc tttccataaa cgcataggcc ttatctgctg
     6961 tccccatatc gatggccatc atccccccga aaccgaagtc ggggttcatc aggcttttca
     7021 tcagatcatg atctttatgc gatgacaggc cgggatagat gatcctcagg ccatcctttt
     7081 caagcctgtc gctcaggtac agagcatttt cgctgtgctt ccgcatccgt atatgaaggg
     7141 tatgaagatt tttcagaatg g
//
LOCUS       DAOPNH010000175         7137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11906_length_7137_cov_8.022875, whole genome shotgun sequence.
ACCESSION   DAOPNH010000175 DAOPNH010000000
VERSION     DAOPNH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11906_length_7137_cov_8.022875"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKZ74_09695"
     CDS             complement(<1..617)
                     /locus_tag="PKZ74_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPM93071.1"
                     /translation="MALLTQVFPQNVSIKVKGSDTVLPLTQKEAEVFMKKFPGSSVMV
                     TGGGSGVGLAALQNGTADIAQSSRKMKLDEKMKMQDAGKAFTEVIIANDALAVIVHPS
                     NPVTQMTREQIEGIYTGKITNWKDVGGEDLKIVMYSRETSSGTYEFFKEHVMNKKNFA
                     PTAMLMPATGAIVQSVSQTKGAIGYVGLAYLESDIRPVKVSYDKG"
     gene            complement(716..2065)
                     /locus_tag="PKZ74_09700"
     CDS             complement(716..2065)
                     /locus_tag="PKZ74_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPM93072.1"
                     /translation="MVITVNHKLTISLFLIWIFALPFVSYSQVNPENDVNLAVDTLNA
                     KVETVKSDMDLAKKLKISGYVQAQWQLADTAGQSSSFSGGNFPKNSDNRFQLRRGRIK
                     FGYKGKLTQFVFQIDATQSGVALRDAYMNFTDPWLQMFTVTGGLFKRPFGYEVLQSSG
                     DRETPERSRIAQSLFPGERDLGAMITIQPRKESRFNFIRLSAALISGNAIAPEIDSRK
                     DFVGQLGINRTTRNEAFRYAMNVSYYNGGYFQETKKVYTLESLANGIYGYIVDSTETN
                     KGEYAKRQYFGADAQFVYDWKPGTTTIRGEYIFGDQPGSAKSNLSPTNRTFSSTDPLD
                     TYNRHFSGGYVYFIQNILHSRHEIVIKYDWFDPNTRVAGNEIVSAVTVEEASVKTDMS
                     AADIMYSTWGFGYSVRIASNVKLTAYYEMVKNETTQLAGFAKDVKDNVLTIRAQFKF"
     gene            2414..4798
                     /locus_tag="PKZ74_09705"
     CDS             2414..4798
                     /locus_tag="PKZ74_09705"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPM93073.1"
                     /translation="MSEPFVHIYNTDSEADTCRREVLPRLYASQWTDDMILEQRNFTD
                     GKIIIVGRKAKRKKARRFDYLLRYSLNFPIAIVEAKVKYKEAGEGIQQAKEYAQILGL
                     KFAYSTNGTEIIEFDSITGIEKFISQFPTPNELWNRLKDDEPIKKEIEQIFLKPFFTV
                     PGKEMRYYQRNAVNKAVKAILEGRKRTLLTLATGTGKTTIAFQIIYKLWNNRWNIRGD
                     QRRPKVLYLADRSVLVTDPHAKDFAVFGDARCLVPDEGLPSSRDIYFSTYQSLAEDDN
                     RVGAFRQLSRNFFDLIVVDECHRGSATDDSNWRIILNYFNDAVKLGLTATPLRDDNID
                     TYHYFGNPLYTYSLKQGIEDGFLAPYIVHRIVPEPDALGFRPQEGQLDANGELIPDGI
                     YTTPDFDTILSHLPRTKAVAKHLFDFMVKYGRFDKTIVFCVNQEHADHFRREISNLNS
                     DMVSQYPDYVVRIVADEKEIGKGHLGKFMDIDEPIPVVVTTSRLLSTGVDVQTCKNIV
                     LFREVNSMTEFKQIIGRGTRVREDKEKLFFTILDYTGSATRKFADPVFDGDPPLITEE
                     EIDENGDLKPGTDITISDPAHPEEDVSISDPPHPPGEKRKKYYIREGEVRIVAESVQI
                     TGADGKLRTIHFTQYAREQIATLFPSVEELRSKWSNVEQRNFIFEQLEDCGISVEQLM
                     DITHLSEADPFDLLCFVAYDLKPKTRKQRADLIRRNKTDFFAEYSVKAQHILQLILEK
                     YVEFGLNQIRPDIISSVPINAEGNEIEIVNAFGGIEIFRKAIEQLQVLLYEEAA"
     gene            4803..5657
                     /gene="dinD"
                     /locus_tag="PKZ74_09710"
     CDS             4803..5657
                     /gene="dinD"
                     /locus_tag="PKZ74_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HPM93074.1"
                     /translation="MKKEQIAQLFEQFENSCQLWHGIECWSARELQEIFNYSDWRNFL
                     KVVEKAKKACENAGSVVTDHFVEFNKMVQIGSGTQREIEDIILTRYACYLVAQNGDAS
                     KSEIAFAQTYFAVQTRKQEVIEQRLLDIARVSAREKLTKTEKKLSGIIYERGVDEQGF
                     AIIRSRGDKALFGGFTTQDMKRKLNVPENRPLADFLPTLTIKAKDFATELTSHNVVEK
                     DLKGESAISKEHIDNNLAVRKILQERGVKPEQLPPAEDVKKIKRKLESEEKKVLKESK
                     KLYKKKNS"
     gene            5741..>7137
                     /locus_tag="PKZ74_09715"
     CDS             5741..>7137
                     /locus_tag="PKZ74_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPM93075.1"
                     /translation="MRKDKGLNGDGDRLPMLTWIMFLKFLDDNEFLQETQANLKNKNY
                     RAAIDAPYRWRDWAGDKNLTGDDLLAFINNEKVLLADGNEASGLLYFLRGLQSESGTE
                     RRDVIATVFKGVTNRMINGYLMRDVINKIDEIHFTNNEEINTLSHLYESILKEMRDAS
                     GDAGEFYTPRPVVRFMVEMLDPKIGEMVLDPAAGTGGFLVESFEHLKKQAKTVEQQEI
                     LQSKSIQGGEAKPLPYLLCQMNLLLHGLNYPDIDSGNSLRKPLRDIGDSERVDIILTN
                     PPFGGEEERGILNNFPDDKKTAETALLFLQLIMRKLKRKTPVLNGGRAAVVVPNGTLF
                     ADGIAARVKKQLIDEFNLHTIVRLGEGVFAPYTDIPCNLLFFQHGKPTTEIWYYELQP
                     PADRKKYSKTRPIQQEEFTELKRWWLDRQENDNSWKVLLKDILFTDEAGKLLNVNLDI
                     KNPNRKNEFEYREPG"
BASE COUNT         2154 a         1386 c         1656 g         1941 t
ORIGIN      
        1 tgtcctttat cgtagctgac cttaaccggc ctgatgtcgg attccaggta ggccaggccc
       61 acgtaaccga ttgcgccttt ggtctggctg accgactgta cgatggcccc cgtggccggc
      121 atcagcatgg ccgtcggcgc gaaatttttc ttgttcatga catgttcctt aaagaactca
      181 taggtacccg aactggtctc cctggaatac ataacgattt tcagatcttc acctccaaca
      241 tctttccagt tggttatttt accggtgtag atcccttcga tttgctcgcg ggtcatttgg
      301 gtgaccgggt ttgaagggtg cacaatgacg gccagcgcat cgttggcaat gatcacctcg
      361 gtaaaggcct tgccggcgtc ctgcatcttc attttttcat ccagtttcat tttacgcgac
      421 gactgcgcga tatcggctgt gccattctga agcgctgcca ggcctacacc tgaaccaccc
      481 ccggtaacca tcaccgatga tccgggaaac tttttcataa atacttctgc ttctttctgt
      541 gtaaggggta gtaccgtgtc gctccctttt accttgatgc tgacattctg agggaacacc
      601 tgcgtaagaa gggccatgaa tacgattgct aatgttattt ttttcatttt tattatttta
      661 tttaacagtt tataaaccaa tactttaatc aggaacttat attaagaatt tacaattaaa
      721 atttaaattg tgctctgatt gtcagcacat tgtctttcac atctttagca aagcctgcca
      781 gttgggttgt ttcattttta accatttcat aataagcggt tagtttgaca ttggacgcga
      841 tacggacact atagccgaat ccccaggtgg agtacatgat gtcggcggcg ctcatatcgg
      901 ttttaacact ggcttcttcg accgtcaccg cagacacgat ttcattgccc gctactctgg
      961 tattgggatc gaaccaatcg tatttgatca cgatttcgtg cctggagtgg agaatattct
     1021 ggataaaata tacgtatcca ccactgaaat gtctgttata tgtgtcaaga gggtcggtgg
     1081 aggaaaacgt ccggtttgta gggctgaggt tagatttggc agaaccaggc tgatcgccga
     1141 agatgtattc gccccggatg gtggtcgtcc ccggtttcca gtcatagacg aactgggcat
     1201 ctgccccgaa gtattgcctt ttagcatact cccctttgtt tgtttccgtc gaatccacaa
     1261 tatatccgta aattccgttt gccagcgatt caagggtata aaccttcttt gtctcctgaa
     1321 aatagccacc attatagtag gatacattca ttgcatacct gaacgcttcg ttgcgggttg
     1381 tgcggttaat gccgagttga ccgacgaagt ctttccggga atcgatttcc ggcgcaatag
     1441 catttccgct gattaaagct gcgctcaggc ggatgaaatt gaacctcgac tctttacgtg
     1501 gctgaattgt tatcatggcg ccaaggtcac gctcgccggg gaagagcgac tgagcgatcc
     1561 tcgatctttc gggagtctcc cggtcaccgg atgattggag gacctcataa ccgaacggac
     1621 gtttgaagag cccgccggta acggtaaaca tttgcaacca gggatcggta aaattcatat
     1681 aggcatctct cagtgcgaca ccgctttgtg tggcgtcaat ctgaaataca aattgtgtca
     1741 gtttaccttt ataaccgaat ttaatgcggc ctcttcttaa ttggaaccgg ttatccgaat
     1801 ttttcgggaa atttcctcct gaaaaggatg aactttgacc tgccgtgtcg gccaattgcc
     1861 attgagcctg gacgtatccg gagattttca gtttttttgc caggtccata tcagacttaa
     1921 ctgtctcaac ttttgcgttg agcgtatcga ctgcgagatt cacatcattt tcaggattta
     1981 cctgtgaata ggatacaaac ggaagcgcaa agatccagat cagaaataaa ctgattgtta
     2041 atttgtggtt tactgttatt accataatgt taaggtttgg taaattttgg tgcaaaagtc
     2101 catccttaac attaagttaa catcagggta atattaagaa attgttaagc agatgataat
     2161 taattaatat ccaatgatat aataagggtt tgtctcttta attcgtgtga ctctttttta
     2221 ttgcagtccg acggatgctg atattctctt ccgacgggta aacccatcgg ttcagagttt
     2281 tttctgacga ctgaagccgt cggtttgaat aaggatttct tccgacggct aaagccgtcg
     2341 gttaggacct acattagttt tttagatgga atagcataaa attaattaat ttaaccgatt
     2401 atttttaaat gccatgtctg agccttttgt acatatctat aatacagata gcgaagcaga
     2461 tacttgccga agagaagtcc tgcccagact ttatgccagt caatggacag atgatatgat
     2521 tttggagcaa cggaacttta ctgatgggaa aattattatt gtagggagga aggctaaaag
     2581 aaagaaagcc aggcgttttg attatttgct gcgctattca ctgaattttc ctattgccat
     2641 agttgaagcc aaagtgaaat acaaagaagc cggagaagga attcagcagg caaaagaata
     2701 tgcacaaatc ctggggctta aatttgctta ttcaactaac ggaacagaaa taatagaatt
     2761 tgattcaatt acggggatag agaaattcat ttctcagttt cccaccccaa atgagctatg
     2821 gaacaggttg aaggatgatg aaccaattaa aaaagaaatt gaacaaatat tcctgaagcc
     2881 attttttaca gttcctggaa aggagatgcg ttattatcaa agaaatgccg ttaacaaagc
     2941 ggttaaggca attcttgaag gaaggaaaag aactttattg acactggcca caggtacagg
     3001 aaaaaccacc atagcattcc agattattta taaactatgg aataacaggt ggaatatcag
     3061 gggggatcaa agaagaccaa aggtattata tctggcagat cgcagtgtcc tggtaacaga
     3121 tcctcatgct aaggattttg ctgtttttgg cgatgcgaga tgccttgttc cggatgaagg
     3181 attgcccagt agccgtgata tatatttttc aacttaccag tctttagctg aggatgataa
     3241 ccgagttggc gcatttcggc aactttcgcg caactttttt gacctgatag tcgttgatga
     3301 atgccatcgt ggtagtgcaa ctgatgatag taattggaga ataatactga actattttaa
     3361 tgatgcagta aaattaggat tgaccgcaac acctttaagg gacgacaata tcgacacgta
     3421 tcattatttt ggaaatccac tttacacgta tagtttaaaa caagggatcg aagatggctt
     3481 tttagctcct tatattgttc atcggatagt tcctgagcca gatgcccttg ggttcagacc
     3541 tcaggaagga caactggatg ctaacggaga gttgatacct gatggaatct atacaactcc
     3601 tgattttgat actattttat cacacctgcc caggaccaag gctgtcgcca aacatctttt
     3661 tgatttcatg gttaaatacg ggagatttga taaaacgatt gtattttgtg tcaaccagga
     3721 acatgcggat cacttcagaa gggaaatcag caaccttaat agcgatatgg tttctcaata
     3781 tccggattac gttgtaagga ttgtggccga tgaaaaggaa atcggaaaag ggcatcttgg
     3841 gaagtttatg gatattgatg aaccaatacc ggttgtcgta accacttcaa gattattaag
     3901 cactggtgtt gatgtgcaga cctgcaaaaa cattgtttta ttccgtgagg taaattcaat
     3961 gacggaattc aagcagatta tcggaagggg gacaagggta agagaggata aagaaaagct
     4021 attctttact attctggatt acaccggcag tgcaacaaga aaatttgctg atccggtatt
     4081 tgatggtgat cctccattaa ttacggaaga ggaaattgat gaaaacggag atttaaaacc
     4141 aggaacagat ataacaatca gtgatcctgc ccatccggag gaggatgtta gtatttcgga
     4201 tcccccacat ccacccggcg agaaaagaaa aaagtactat atcagagaag gtgaggtccg
     4261 gattgtagca gaaagcgttc agataaccgg ggccgatgga aaattaagaa caattcactt
     4321 tacacagtat gccagggaac agattgcaac attatttcct tcagtcgaag aactcaggag
     4381 caaatggagc aatgttgaac agcggaattt tatttttgaa caacttgagg attgtggaat
     4441 ttcagttgaa caactcatgg atatcactca tctttctgaa gcagatccct ttgacttact
     4501 ttgttttgtt gcttatgacc tgaaacctaa aacaagaaaa caaagggctg atttaattag
     4561 gaggaacaag actgatttct tcgctgaata cagcgttaaa gctcaacata tactacaact
     4621 aatactggaa aagtatgtcg aatttggttt aaatcaaatt cgtccggata ttatttcctc
     4681 agttcccatt aatgcggaag gaaatgaaat tgaaattgtc aatgcctttg gaggaattga
     4741 aattttcagg aaggcaattg aacaacttca ggttttactt tacgaagaag cagcgtagtt
     4801 ttatgaaaaa agagcagatt gcacaacttt tcgagcaatt cgaaaatagc tgccaattat
     4861 ggcatgggat agaatgctgg agcgccaggg aattgcagga gattttcaat tattcagact
     4921 ggagaaattt cctcaaagta gttgaaaaag caaaaaaagc ttgcgaaaat gccgggagcg
     4981 tagtaactga ccatttcgtt gaattcaaca aaatggtaca gattggcagt ggcacacaaa
     5041 gagagatcga agatattata ttaacgcgat acgcttgcta tctggttgcg caaaacggag
     5101 atgcttccaa aagtgaaatt gcttttgcac aaacctattt tgcagttcaa acccgtaagc
     5161 aggaagttat agagcaaaga ctgcttgata tagccagggt ttccgcgaga gaaaagctga
     5221 cgaaaacgga aaagaagctt tcaggaatta tatatgaaag aggtgtagat gaacagggat
     5281 ttgctattat ccgttcaaga ggtgataaag ccctttttgg gggttttaca acgcaagata
     5341 tgaaacggaa gttaaatgtc ccggaaaaca ggcccctggc cgatttcctc cctacattga
     5401 ccattaaagc caaggatttt gcaacagaat tgaccagtca caatgttgta gaaaaagatt
     5461 tgaaaggaga atcggccata tccaaagaac acatcgacaa taaccttgca gtgaggaaaa
     5521 ttttacagga aagaggagta aagcctgagc aattgcctcc cgctgaagat gttaaaaaga
     5581 taaaacgaaa actggaaagt gaagagaaga aagttttgaa agaaagcaaa aaactttata
     5641 agaagaagaa ttcatgagca aagagaagaa ggcaaagtta ttggtgaagg aaacacccgc
     5701 acagcgttta tcgggtataa ttaaatcatg ccgcaatata atgcggaaag ataaaggatt
     5761 gaatggcgat ggcgaccggt tgccgatgct tacctggatc atgttcctga agtttctgga
     5821 tgataatgaa tttctgcagg aaacccaggc taatcttaaa aataagaatt accgggcggc
     5881 gattgatgct ccatacagat ggagagattg ggcaggagac aaaaatctga ccggcgatga
     5941 tttgcttgct tttattaata atgaaaaggt tctgttagcc gatggaaacg aagcgtctgg
     6001 tttgctttat ttcctgagag gactgcaaag cgaatcgggg acggaacgaa gagatgttat
     6061 agcaacggtt tttaaaggtg tgacgaaccg tatgatcaat ggctatctaa tgcgcgatgt
     6121 tattaacaag atagatgaaa tacacttcac gaacaacgaa gaaatcaata ccctttccca
     6181 cttgtatgaa agcattttga aagagatgcg ggatgcaagc ggagatgcgg gtgagttcta
     6241 caccccaagg cctgtggtga gatttatggt agaaatgctg gacccgaaaa ttggagaaat
     6301 ggttttggat cctgcagccg gtaccggagg atttctggtt gaatcctttg aacatttgaa
     6361 aaagcaggcc aaaaccgttg aacagcagga aatattgcaa agcaaatcca ttcaaggagg
     6421 ggaggccaaa ccgctgcctt acctgctttg ccagatgaac ctgctgttgc atggattgaa
     6481 ttaccccgat atcgacagcg ggaacagttt acggaaacct ttgcgcgata ttggcgacag
     6541 cgaaagggta gatattatcc tgaccaatcc gccatttggc ggggaggagg agaggggtat
     6601 tctgaacaat ttcccggatg acaagaagac cgccgaaacg gcgttgcttt ttctccaact
     6661 gatcatgcga aagctaaaac ggaaaacacc tgtactaaat ggaggcaggg cagccgtggt
     6721 ggttcccaac gggacgctgt ttgccgatgg cattgcagcc agggttaaga agcaactcat
     6781 tgatgagttt aatctgcata cgattgtacg gcttggagaa ggcgtatttg ccccctacac
     6841 tgatattccc tgtaatcttt tattctttca gcacggcaaa cccacaaccg aaatctggta
     6901 ttacgagctg caaccgccgg ccgaccggaa gaaatacagc aaaacccggc ctatccaaca
     6961 ggaagagttc accgaattga aaagatggtg gctcgaccgg caggaaaatg ataattcctg
     7021 gaaggtcctg ttaaaagaca tattgtttac cgatgaagcc ggaaagttac tgaatgtcaa
     7081 cctcgacatt aaaaatccaa accggaaaaa tgaatttgaa taccgggaac cggggga
//
LOCUS       DAOPNH010000176         7131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11923_length_7131_cov_7.403618, whole genome shotgun sequence.
ACCESSION   DAOPNH010000176 DAOPNH010000000
VERSION     DAOPNH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11923_length_7131_cov_7.403618"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKZ74_09720"
     CDS             <1..439
                     /locus_tag="PKZ74_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93076.1"
                     /translation="DAGASVTYLTEGIAPFRVFTTEWYNVLSYSTGSASRLNFQVKLY
                     ETLNIIEFYYGTAISGYNSPDEGASIGIKDAMGGQGHYIEATQNSTDIILAFLNSSTD
                     WPAQNFRFIPPLTTDMEIFHKIIVNKPGANLSIQKDIKITGLE"
     gene            501..2498
                     /locus_tag="PKZ74_09725"
     CDS             501..2498
                     /locus_tag="PKZ74_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPM93077.1"
                     /translation="MKKISLIMLITGFVFSGFYAQSQRYDKGAQYCSHRKSMLKNTPA
                     NVRSENSPRHRFDVLNYKLNMDLWDNFQNPFPQAFDATETVTFRVDTALNQIKLDAVN
                     TSLTIHAVGLAGIGYTHFDDTLTVQLDRIYNPGEIAEVSIDYSHKNVEDEAFYVSGGF
                     VFTDCETEGARKWFPCYDRPADKATVDITAKVPGNVLLGSNGRLADSVTVADTTWYRW
                     ISRDPVATYLVVVSASNNWDLDIVYWDRPSTPGEPMPIRFYYETGDNPVPMENIITDM
                     ATYFSDHYGEHPFEKDGFASVGQEFSWAGMENQTLTSICPGCWYESLICHEFAHQWFG
                     DMISPGTWADIWLNEAFATWSEAFWYENDGGYQAYLNEIQGNASYYLATNPGWAVYEP
                     EWAVETPDNTILFNYSITYMKSSCVLHLLRYALGDSLFFPAIMDYATDTAEFKYKNAI
                     TDDFQAKMEESTGEDLDWFFDSWVKQPNHPVYENIYSIHDNNNGTWNVNFQVNQVQDN
                     AGFFPLPVEISVFFAKYTDTTLRVMNDVNNQSFTFTFDKEPGVVFFDLDNKIVLKQAS
                     LMVGTPEPEKAGKSLFLEQNFPNPVKNSTTLTYTIPESGAVELVIYSTTGNSIKVLVN
                     EVQEKGTHQVTADLSYLPDGLYFCRLQASGQESVIKLAVNH"
     gene            complement(2495..2953)
                     /locus_tag="PKZ74_09730"
     CDS             complement(2495..2953)
                     /locus_tag="PKZ74_09730"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HPM93078.1"
                     /translation="MKRRVNWGMVLSHHPPGQYNRTFTLCRVRFCVRCTGILAGILLW
                     AIVLSVTDLPFYLTFITGFLFPLPAILNFTLNELGKRKNSNSKRTITGILMGVAIGMA
                     IGFFIANRFWPGITIIAWIILLEFAVALVLRQAGVLDKFIREYEDAVYKD"
     gene            complement(3322..6684)
                     /gene="mfd"
                     /locus_tag="PKZ74_09735"
     CDS             complement(3322..6684)
                     /gene="mfd"
                     /locus_tag="PKZ74_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPM93079.1"
                     /translation="MKTEELIRLYAAAPQVQAMAGILKNQTSPRIFLQGLVGSSPALI
                     KAAVFHLLQRNHLVILNDPESAAYLYNDLETLFGEKGQPYEHKNTLFFPTVYKRHFEF
                     DKPDRTNMLMRSEVLSRLVSTNKKTLIVSYAEALSEKVVRKKVLTKNTLKLRAGEKVS
                     LDFVADMLIEYGFDREDFVYEPGQFSIRGGILDVFSFAHDNPFRIEFFGEEVESIRTF
                     DPASQLSIDKMDRITILPDLSERLEVESRESFLSFLPPGAMIWVDDLAGLQDLLDVYT
                     DRLQKLLDSDRDGLYPKNLLGHFTGKDDFLKEMLGHAIVESGSRPVLKPDLAPEFDIR
                     PQPTFNKNFDLLTADLAVNTKAGISNFILSENPRQIERIYTIFEDLKQSRKQPEDFEF
                     STLNISLHEGFIDRKLNLALYTDHQIFERYHKFHLKDSYSTREAITLKELYNLSPGDY
                     VTHIDHGVGRYDGLERVDVNGRQQEAIRIIYKNNDLLYISIHSLHRISKYIGKEGTEP
                     KIDRLGSGAWSKLKAKTKSRVKDIAKELIQLYAQRKKTDGFRFLPDTYLQTELEASFI
                     YEDTPDQLKATVDVKADMEKEYPMDRLICGDVGFGKTEIAVRAAFKAVADSKQVAVLV
                     PTTILALQHYKTFRDRLKDFPCRVDYLNRFKSQKKQKETLRELEEGKLDIIIGTHRLI
                     SQDVKFKDLGLLIIDEEQKFGVGAKEKLKQIKVNVDTLTLTATPIPRTLQFSMMGARD
                     LSIISTPPPNRYPIQTEVRPFSEEVIREAILYEVSRGGQVFFVHNRVLNIHDVERMLK
                     KFLPNITIGIAHGQMDGTHLENVMLDFIDGVYDVLLATTIIESGLDIPNVNTIIINDA
                     HHFGLSDLHQLRGRVGRSNRKAFCYLLAPPVSMLTDEARKRLRAIEEFSDLGSGFNIA
                     MRDLDIRGAGNILGAEQSGFISEIGFEMYHKILDEAMEELKESEFSELFRDEKPKPFV
                     KDCQIETDLEILIPADYITNSTERLIIYKELDGMENEEELAACKNRISDRFGPVPRQT
                     EELFNAIRLRWLAKEAGFEKIILKNERFIGYFISNQESPYYRSEKFTAMLRFVQENPS
                     ICRMKENESRLSLTFRNVRSIRDALALMYRIN"
     gene            6754..>7131
                     /locus_tag="PKZ74_09740"
     CDS             6754..>7131
                     /locus_tag="PKZ74_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93080.1"
                     /translation="MKVFKFGGGVMNSAGAVGRIPEIIRRYPGIPLVIVVSAFGKTTN
                     ALEELIASTISADGRSGRLFRQIKEYHDEITRQLFPNDSKEPEIRTGAQWNELERITG
                     SEVTGTAAEIYDRMIVFGELISSV"
BASE COUNT         1752 a         1810 c         1772 g         1797 t
ORIGIN      
        1 cgatgccgga gcatccgtca cttacctcac tgaaggtatt gcccctttca gggtgtttac
       61 aaccgaatgg tacaatgttc tgtcgtattc aacaggttcg gcttcccggc tcaatttcca
      121 ggtaaagctg tatgaaacgc tcaacatcat cgaattttat tacggcacgg caatatcagg
      181 ttacaacagc cccgatgaag gagcctctat cggaatcaaa gacgctatgg gcggacaagg
      241 ccattatatc gaagccacac agaattccac cgatattatc ctggcattcc tgaacagcag
      301 caccgactgg cccgctcaga atttcaggtt tattcctccc ctgacaaccg atatggaaat
      361 cttccataaa attatcgtca acaaaccggg cgctaatctc tccatccaga aggatataaa
      421 aataacggga ctggaataaa ttcttaccag gctccgttca aatctttttt aggattcttt
      481 cattaccttt gagttttttt atgaaaaaga ttagtttgat catgctgatc actggatttg
      541 ttttcagtgg tttttatgcc caatcccaac gttacgataa aggggcacag tattgttccc
      601 acaggaagtc gatgctgaaa aacactcctg ctaatgtcag aagtgaaaat tcaccccgtc
      661 accgcttcga tgttctgaat tacaaattaa atatggatct ttgggataac ttccagaatc
      721 ctttcccgca ggcctttgat gcaacggaaa cggttacctt cagagtcgac acagcgctga
      781 accagatcaa gctcgacgct gtcaacacct ccctgaccat tcatgcggtt ggactggcag
      841 gtattggtta tacacatttc gatgatacgc ttactgttca actggaccga atctacaatc
      901 ccggggaaat cgccgaggtt tccattgatt acagccataa aaacgtggaa gatgaagcgt
      961 tttatgtcag cggcggtttt gtttttaccg actgcgaaac ggaaggcgcc cggaagtggt
     1021 ttccctgcta cgaccggcca gccgacaaag ccactgtgga tatcactgca aaagttcccg
     1081 gcaatgtcct cctgggttca aacggccggc ttgccgattc ggtcacagta gccgacacga
     1141 cctggtaccg gtggatcagc cgcgatccgg tagccactta cctggtcgtc gtttcggcca
     1201 gcaacaactg ggacctggat atcgtttact gggaccgccc ctccacaccc ggcgaaccga
     1261 tgccgatccg cttttactac gaaactggcg acaacccggt cccgatggaa aacatcatca
     1321 ccgatatggc cacctatttt tcggatcatt acggtgaaca tcctttcgaa aaagacggat
     1381 ttgcttcggt aggccaggaa tttagctggg cggggatgga aaaccagaca cttaccagca
     1441 tctgcccggg ctgctggtac gaaagcctga tctgccatga atttgcccac cagtggttcg
     1501 gcgatatgat cagtcccgga acctgggccg acatctggct gaatgaagcc ttcgccacct
     1561 ggtcggaagc tttctggtat gaaaatgacg gtggatacca ggcgtatctc aatgaaatcc
     1621 agggaaatgc cagctattac ctggccacga acccgggatg ggctgtttac gaacccgaat
     1681 gggcggtgga aacccccgat aacactatcc tgttcaacta ttcgataacc tacatgaaaa
     1741 gctcctgcgt gctccacctg ctccggtatg ccctcggcga cagccttttc ttccccgcca
     1801 tcatggatta tgccaccgat acggcggaat tcaaatataa aaacgcaatc accgacgact
     1861 tccaggcaaa gatggaagag tcgaccgggg aagatctcga ctggtttttc gactcctggg
     1921 taaaacaacc caaccatccg gtttacgaaa atatttactc catccacgac aataacaacg
     1981 gaacatggaa tgtaaacttc caggtgaacc aggtgcagga taatgccggc ttcttcccgc
     2041 tgccagtcga aatatcggtt ttctttgcca aatacaccga tactacgctc cgcgtcatga
     2101 acgacgttaa taaccagtcc tttaccttca cgtttgacaa agagccgggc gtagttttct
     2161 tcgacctcga caacaagatt gtcctcaagc aggcatcgct gatggtgggc actcccgaac
     2221 cggaaaaggc cggcaaaagc cttttcctgg aacaaaactt cccgaacccg gtgaaaaatt
     2281 ccacaacatt gacctatact attccggaaa gtggggcagt agaacttgtt atctattcga
     2341 caaccggaaa tagcataaaa gtgctggtga atgaagtaca agaaaaaggg actcaccagg
     2401 tcacagccga cctttcatat ttgccggatg gcctttattt ctgccgtctt caggcttccg
     2461 gacaagaatc cgtcatcaaa cttgccgtaa atcattaatc tttatagaca gcatcttcat
     2521 attcccggat aaatttatcc aatacaccgg cctggcgaag gaccaatgca actgcaaatt
     2581 ccaggagaat gatccaggcg atgatcgtga taccgggcca aaatctattt gcaataaaga
     2641 atccgatggc catacctata gccactccca tcaaaatccc cgtaatagtt cgtttactgt
     2701 tcgaattttt ccgtttgccc agttcattaa gggtaaaatt gaggatggca ggcaggggaa
     2761 acagaaatcc tgtaataaaa gtgagataaa aaggaaggtc cgttacggac aatacaatcg
     2821 cccatagtaa aattcccgcc aggatacccg tacacctgac acaaaaccgg acccggcaca
     2881 gggtgaaggt ccggttatat tgaccagggg gatgatgcga aagcaccatc ccccagttca
     2941 ctcttctctt caagaaaaaa tgctatttgc ccatatcatt tgcccttgca ggaccgaagt
     3001 tatctccgtc gcaaatgggg cattttgctt tcttcagcaa atagtaaggc acataaaata
     3061 ttccacacag gaatataaag accagctagt taaacttttt cttcggaaca acattcctgt
     3121 tacacagatt acaccacttt ggagaggcca tagcttttag tttttagtta ataatggagt
     3181 tatttatctg gttattattt acccgtttta taaaaaatct taatccattt ttaggtgagg
     3241 taaatttatt gataaatatt taacagatgg aacaaaactt gaaaaaaaat catgaaaggc
     3301 cataaatctg ccaggaaggc gctaattaat cctgtacatc aatgcaagag catcacgtat
     3361 tgacctgaca ttgcgaaagg taagcgaaag ccggctttcg ttctccttca tacgacagat
     3421 agagggattc tcctgaacaa agcgcagcat ggccgtaaat ttctccgacc ggtagtaggg
     3481 cgactcctgg ttgctgataa aatatccgat aaaccgttca tttttgagga tgattttttc
     3541 aaaaccggct tctttggcga gccatcgaag gcggatggca ttgaaaagct cctcggtctg
     3601 gcgcggcacc gggccgaaac ggtccgaaat ccggttcttg caggcagcca gttcctcttc
     3661 gttctccatt ccgtcgagct ctttgtaaat gatcagcctt tcggtggagt tggtgatgta
     3721 atcggcggga atgaggattt cgaggtcggt ctcgatctgg caatccttta cgaagggttt
     3781 gggcttttca tccctgaaga gttcgctaaa ttccgactcc ttcagctctt ccatggcttc
     3841 gtcgaggatc ttgtggtaca tctcgaagcc gatttccgag ataaacccgc tctgttcggc
     3901 ccccagtata ttccctgccc cgcggatatc gaggtcgcgc atggcgatgt tgaagccgct
     3961 gcccagatcg gagaattctt cgatggcgcg gagccgtttg cgggcttcgt cggtaagcat
     4021 ggagacgggc ggcgccagca ggtagcagaa ggctttccgg ttggagcgcc ctaccctgcc
     4081 gcgcagctgg tgcaggtcgc tgagcccgaa atgatgcgca tcgttgataa tgatggtatt
     4141 cacgttcggg atatcaaggc ccgattcgat gatcgtagtt gccagcaaaa catcgtacac
     4201 gccatcgata aaatcgagca tgacgttctc caggtgcgtc ccgtccatct gtccatgggc
     4261 tatgccgatg gtgatgttgg ggaggaattt cttcagcatc cgctccacat cgtggatgtt
     4321 caacacccgg ttatggacga aaaacacctg tccgccgcgc gacacctcgt acaggatcgc
     4381 ttcgcggatg acctcttcgc tgaacggcct gacctccgtt tgaatcgggt accggttcgg
     4441 cggcggggtg ctgatgatcg acaggtcgcg cgcgcccatc atggaaaact ggagcgttct
     4501 tgggatcggc gtggccgtca gcgtcagcgt atcgacattt accttgatct gctttaattt
     4561 ctctttagcc cccacgccga atttctgctc ctcgtcgatg atcagcaggc ccaggtcttt
     4621 gaatttcaca tcctggctga tcagccggtg tgtcccgatg ataatgtcaa gcttgccctc
     4681 ttccagttcg cgcaacgtct ccttttgctt cttctggctt ttgaaccggt taagataatc
     4741 gacgcggcag ggaaaatctt taagcctgtc gcggaaagtt ttgtaatgct gaagggccag
     4801 gatggttgtc ggcaccagga ccgccacctg tttgctgtcg gctacggctt taaaggcggc
     4861 cctcaccgca atctctgtct tgccgaaccc gacatccccg cagatcagcc ggtccatcgg
     4921 gtactccttc tccatatcag ctttaacatc cactgtggct tttagctggt caggggtatc
     4981 ttcataaatg aatgaagctt ccagttcggt ctgcaggtag gtatcgggca aaaagcggaa
     5041 cccgtccgtt ttcttgcgct gggcataaag ctggatcagc tccttggcaa tatccttaac
     5101 gcggctcttg gttttggctt tcagtttgct ccaggccccc gatcccagcc ggtcgatctt
     5161 cggctccgtc ccctccttcc cgatatattt tgagatccgg tgcagcgagt ggatgctgat
     5221 ataaagcagg tcgttgttct tgtagatgat gcggatggct tcctgctgcc ttccgttgac
     5281 atcgacgcgc tccagcccgt cgtaccgtcc gacgccatga tcgatatggg ttacataatc
     5341 accggggctg agattgtaaa gctctttcag ggtaatcgcc tcccgtgtgc tgtaactgtc
     5401 tttgagatgg aatttgtggt agcgctcgaa tatctggtgg tcggtgtaga gcgccaggtt
     5461 cagcttcctg tcgataaagc cttcgtgcag cgagatgttg agtgtcgaaa attcgaaatc
     5521 ttcgggctgt ttccgcgact gtttcaaatc ttcaaagatg gtatagatcc gttcgatctg
     5581 gcggggattt tccgacagga tgaaattgga gatgccggct tttgtattga cggccaggtc
     5641 ggcggtaagc aggtcgaagt tcttgttgaa agtgggttgt ggccggatat cgaattcggg
     5701 ggcgaggtcg ggtttcagta ccggccggct gcccgattcg acgatggcat gaccgagcat
     5761 ctctttcagg aaatcgtctt tgccggtaaa atgcccgagg aggtttttgg gatacaggcc
     5821 gtccctgtcg ctgtcaagca gtttttgcag cctgtcggta taaacatcca gtaaatcctg
     5881 caatccggcc aggtcatcca cccagatcat ggctccgggc ggcaggaacg acaggaacga
     5941 ttcgcgggat tccacctcaa ggcgctccga aagatcgggc aagatcgtga tcctgtccat
     6001 tttgtcgata gaaagctgtg atgccggatc gaaggtacgg atcgactcca cctcttcccc
     6061 gaagaattcg atacggaaag gattatcgtg ggcaaaggaa aaaacgtcga ggatgccgcc
     6121 gcgaatggag aactggcccg gctcatagac aaaatcctcc cggtcgaacc cgtattcgat
     6181 cagcatatcg gccacaaaat caagcgatac cttctcccct gccctcagtt tcaaggtgtt
     6241 ctttgtcagc actttcttgc ggaccacttt ttccgacaag gcttcggcat aactgacgat
     6301 cagggttttt ttattggttg ataccagccg gctcagcacc tccgaccgca tcagcatatt
     6361 ggtccggtcg ggtttatcga attcaaaatg gcgtttatag accgtgggaa agaagagggt
     6421 gtttttgtgc tcatagggct gccctttctc tccgaaaagg gtttccaggt cattatagag
     6481 ataggctgcc gactcgggat cattgaggat gacgagatgg ttgcgctgga gaagatgaaa
     6541 gacggctgct ttgatcaggg ccggggatga cccgaccagc ccctgcagga aaatcctggg
     6601 ggaggtctgg tttttcagga tgccggccat ggcctgtacc tggggcgcgg cagcgtaaag
     6661 gcggataagt tcttctgttt tcaaccgttc aaattaccct tacagttctg caaaagtatt
     6721 atttttgccg gtagcgatca cgaattaatc ccgatgaaag ttttcaaatt tggtggcggc
     6781 gtaatgaatt ccgccggggc agtgggccgg ataccggaga ttatccgcag atatcccggc
     6841 atccccctgg ttattgtggt ttccgctttc gggaaaacga ccaatgcact ggaggagctg
     6901 atcgcttcga cgatttcagc cgacggcagg tcgggccggt tgttcaggca aatcaaagaa
     6961 taccatgatg aaatcacacg gcagcttttc ccaaatgact ccaaggaacc cgagattcgt
     7021 accggggcgc aatggaatga gctggaaagg attaccggga gcgaagtgac cggcactgcg
     7081 gctgagattt atgaccggat gatcgttttc ggcgagttga tctccagcgt c
//
LOCUS       DAOPNH010000177         7121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_11943_length_7121_cov_6.361308, whole genome shotgun sequence.
ACCESSION   DAOPNH010000177 DAOPNH010000000
VERSION     DAOPNH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11943_length_7121_cov_6.361308"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /gene="rodA"
                     /locus_tag="PKZ74_09745"
     CDS             <1..388
                     /gene="rodA"
                     /locus_tag="PKZ74_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPM93081.1"
                     /translation="LIGKGFLQGTQTKYNFVPEQSTDFIFCTVGEEWGFLGSTAVIGL
                     FVYLLFRLVRMAERQRSDFSRIYGYGVISILFFHFVVNIGMTIGLVPVIGIPLPFFSY
                     GGSSLWAFTILLFIFIKLDANRLALF"
     gene            complement(385..2508)
                     /locus_tag="PKZ74_09750"
     CDS             complement(385..2508)
                     /locus_tag="PKZ74_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPM93082.1"
                     /translation="MAIIGTLRKHSALAVILVGVAIAAFIVSDLFSGKRGRGNGVPAV
                     GTIAGEEITVTDYNRRVDVNLENQKANQNKESLTPGETFDIRQSTWNQYLDEIIMGNE
                     YKKLGLSVTTEELYDLVQGPRPHNLIQQYFKDPQTGMYSPTVVQNFLQNLDNLTPEVR
                     KQWLDLEKYIKEDRLDQKYKSLVTKGYYMPQAFAALDFESKKKNAEVRYVVARYTVIN
                     DDEVTLTDKDYETYYEKNKQSYEQEPSRDIDYLVFDVAPSAKDREDARQEAYRIYDEF
                     RNASDFVTFVNSTSDKRYDSTWYKKGLLPLNIEVALWDAPAGTFVPPYEENNAWHMAR
                     LMDIQARPDSMKAEHILIAFQGALRAGENVTRTKTQAEKTADSIFTIVSADKTKLQAL
                     AYLLSDDGSAKSNNGDLGWFSDGAMIGPFNDAVLNGKVGDVVKVETAFGYHVVKITGK
                     KDPEQKIRVAVIERTIAPSSKTFQDVYAQASTFAGENSTQAKFDAAVSSQGLNKRSAT
                     YLREMGNTIAGIDYPREIVRWAFFDGIEVGDVSPVFDIGGAYVVAVVTAAREKGIMPL
                     EQMKENIKSFVMNEKKASMLKDRIKGIGTDIYQIAREFNTKVDTNLTLTFSSRNIPGF
                     GSEYQVIGEIFSMEAGQQSEPIQGNGGVFVVVVDRFYEPPQPADYNANRDQLLAAFRS
                     RMGANPMFNALQKITKIEDNRLLYF"
     gene            complement(2664..3254)
                     /gene="lptC"
                     /locus_tag="PKZ74_09755"
     CDS             complement(2664..3254)
                     /gene="lptC"
                     /locus_tag="PKZ74_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPM93083.1"
                     /translation="MKTRPSLGENKILLIIIAGIIALMGPAIFSSCKNDVQTVLSLDF
                     VDTLPEMTARDIEILYSEKGQMQIKLVSPYLVNKKGDEELLLFPEGFTVFFYDTAMNL
                     TTKITADYGISYEKKKLMEARHNVVVENLEKEEKLNTEELFWDRAKAVIYSNQFVRLT
                     TGGNVVTGTGLTSKEPFDVLDITHVQGTLEIKDEPQ"
     gene            complement(3266..4846)
                     /locus_tag="PKZ74_09760"
     CDS             complement(3266..4846)
                     /locus_tag="PKZ74_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93084.1"
                     /translation="MKRLFFVITFSLIFAGIPSVNSFGQEDQGAGKYGSDSLTCITNI
                     SLYREFFKQWKASDYKSETIHDLIPPWRWVFLNCPRGTQNTYIDGAKILSYLIESTND
                     SALKYKENKETFNYFDKLKNRYVDTLMMLYDQRIKYFGKEGFVLGRQGVDLFNYRPQD
                     IENIFNYLHRSIELEGDNTLGAILPYYMDAAINLAKERSDSSLIFDAYESSMQIVENN
                     LKKLASESITKNLKVGLNDSETRVYLGVPKSVNKSVTSNGETEQWIYDNLYLYFENGI
                     LTSWQEQNISPDKGNWGLIKASIDFKLEPFATCPDLVTIYRKKFNQTPDDVELLKKIS
                     NLLEQKKCHTDPFYFETTVKLYELEPSPESAYMIGKMLLNEGKYAEAIDYLKQAEKLN
                     DTATVQKSYMYIAKAYQALNNYPSSRAYALKAAALDPTDGDPYMLIGDLYAASARDCG
                     GNDLTNHVAYWVAVDKYQRAKQVEPELGPEADKLIYTYSAYFPATSTIFFYTLKEGDI
                     YRVECWINEDTRIRASKQ"
     gene            complement(4858..6153)
                     /locus_tag="PKZ74_09765"
     CDS             complement(4858..6153)
                     /locus_tag="PKZ74_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93085.1"
                     /translation="MITQSKGKYLLLGLLLVASFRLSSLYGQISINSPYTRYGLGTLV
                     ENGNDPRITAMGGLYSGFRSPSVINTANPASYTAFDSVSFVFDAGVFGLMSNLQVTNQ
                     KNQGSYVSLSHLLFGFPVTRWWRTSFGVLPFSYVGYDIFNTEQQTGMPDIQYVYRGSG
                     GLNQLYWGNGFKIGKKLSVGFNVKYLFGNITRSRGVTFPDSIEMKNTYIRSSMRPSDF
                     YGEIGFQYREKLSKDLFMVIGGVFGPELEIGSSTSRLVTTYFGDITEAQLYYDTIDAV
                     DNEKGSFTLPMRTGGGITLGKDGKWMAGADFTWQNWEKYRYNGNSDSLVNRWSVGVGG
                     EYIPDSRNIGTYFQRVAYRIGFQYGRTPIYLKGKYIDEFGISFGMGLPIKKSKSTVNL
                     AATIGKRGTIQNGLIQENFIKFTIAVNVFENWFFKSKYY"
     gene            complement(6131..6874)
                     /locus_tag="PKZ74_09770"
     CDS             complement(6131..6874)
                     /locus_tag="PKZ74_09770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPM93086.1"
                     /translation="MHRKLIIDIGNTLQKLAVFDGKTMLFKETFDKLKPDSLTGFIAQ
                     HGPFDGIIQSSVIIHTRQLEEILAAAGRFILLTDQTPLPFRNLYRTPKTLGKDRIAAV
                     AGARNLFPGRNCLVIDAGTCITYDLITAEGTYLGGAISPGIRMRFKALHTFTGKLPLI
                     EQEEFDDLIGQTTQESILSGVYNGITGEITHLAGRYHEKSGDLAVVITGGDHHFLHNK
                     LKISIFAAPDLVLLGLNEILDYNDHSIKG"
     gene            7014..7086
                     /locus_tag="PKZ74_09775"
     tRNA            7014..7086
                     /locus_tag="PKZ74_09775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7047..7049,aa:Phe,seq:gaa)
BASE COUNT         1783 a         1653 c         1657 g         2028 t
ORIGIN      
        1 attgatcggc aaggggtttc tgcaggggac acaaaccaag tacaattttg tgccggaaca
       61 gagtaccgat ttcattttct gcaccgtagg cgaagagtgg ggcttcctgg gaagtacggc
      121 agtgataggt ttgttcgttt accttctttt caggctggtc aggatggcag agcggcagcg
      181 gtcggatttc agcaggatat acggatacgg tgtaatatcg atccttttct tccactttgt
      241 agtgaatatc gggatgacga tcgggcttgt gccggtcatc gggatccccc ttcccttttt
      301 cagttacggg ggatcttccc tctgggcatt caccatcctg ctttttatct tcatcaagct
      361 ggatgcgaac aggctggcgt tattctagaa atacaggagc ctgttgtctt ctatctttgt
      421 aattttctga agggcgttga acataggatt agctcccatg cgtgagcgga aagcggccag
      481 cagctgatcg cggttagcat tgtaatcggc cggttgcggc ggttcataga atcgatcgac
      541 caccacgaca aacacgccgc cgttaccctg gatgggttca gattgctgtc cggcttccat
      601 gctgaatatt tccccgataa cctggtattc gctgccaaaa ccgggtatat tcctggaaga
      661 gaaggtcagt gtcaggtttg tatccacttt ggtattgaat tcccgggcga tctggtaaat
      721 gtcggtaccg atgcctttaa tacggtcttt aagcatagag gctttctttt cattcatcac
      781 aaatgatttg atattctcct tcatctgttc cagcggcatg ataccttttt ccctggcggc
      841 ggtaacgact gccaccacgt aagcgccacc gatatcgaat accggtgaaa cgtcgccgac
      901 ttcgatccca tcaaagaaag cccagcgtac aatttcgcgc ggataatcga tgccggcaat
      961 agtattgccc atttcccgca ggtaagtggc cgatcttttg ttcagcccct ggctgctgac
     1021 agccgcatcg aatttagcct gggtgctgtt ttccccggcg aaagtgctgg cctgtgcgta
     1081 aacatcctgg aaagttttgc tgctgggggc aattgttcgt tcaataacag ctaccctgat
     1141 tttctgttcc gggtctttct tgccggtaat tttcaccaca tggtaaccga aagcggtttc
     1201 cactttaacc acatcaccga ctttaccgtt gaggaccgca tcattaaaag gaccgatcat
     1261 ggcgccatct gagaaccatc ccaggtcgcc gttattcgat ttggccgatc cgtcgtcgga
     1321 aaggaggtaa gccagggcct ggagtttggt tttatcagcg gagacaatcg tgaatatact
     1381 gtcggcggtt ttttctgcct gtgttttggt acgggtgaca ttttccccgg ctctcaatgc
     1441 gccctggaaa gcgatcagaa tgtgttcggc tttcatcgaa tcggggcggg cctggatatc
     1501 catcagccgg gccatatgcc aggcattatt ctcctcgtaa ggcgggacga aagtgcctgc
     1561 cggggcatcc cacaaagcca cttcgatgtt gagcggaagc aatccttttt tataccatgt
     1621 actatcgtaa cgcttatcag aagttgagtt gacaaatgtt acaaagtcgc tcgcattgcg
     1681 gaactcatca taaatcctgt aagcttcctg gcgcgcatct tcacggtctt ttgccgatgg
     1741 tgccacatca aaaaccaggt aatcgatatc ccttgaaggc tcctgctcgt aagattgttt
     1801 attcttttcg taatacgttt cgtaatcttt atcggttaga gtaacctcat catcattgat
     1861 aacggtataa cgggctacaa cgtacctcac ttctgcgttc tttttcttgc tttcaaaatc
     1921 cagggcagca aaagcctgag gcatgtaata tcctttggtc accaggcttt tgtatttttg
     1981 atcaagccgg tcttctttga tatatttttc caggtcaagc cactgtttac gcacctccgg
     2041 agtcaggtta tcaaggttct ggagaaagtt ctgcacaact gtcgggctgt acattcccgt
     2101 ctgcgggtct ttgaaatatt gctggatcag gttatgcgga cgcggcccct ggacgaggtc
     2161 gtaaagctct tcagtagtga cagaaaggcc cagcttttta tattcattac ccataatgat
     2221 ttcatccagg tactggttcc aggtactctg ccgaatatca aatgtttctc cgggagttaa
     2281 cgattcttta ttctgattgg ctttttgatt ttcaagattt acatcgacgc ggcggttata
     2341 atccgtgacc gtgatctctt ctccggcgat ggtgcctaca gccgggacgc catttcccct
     2401 gcctcgtttg cccgagaata gatcgctgac gatgaaagcg gcgatagcca cgccgacaag
     2461 aataacggcg agagccgaat gtttacgaag tgttccgata atagccataa ctgcgtattt
     2521 attttaaaaa gaggtgcaaa agtataaaaa aaacgaacgg gtgcaaagta attcatatat
     2581 taatgatgct atccgtcata atagtaaccg gttaagcgac gggtccgggt cgtggataag
     2641 cagaagaaca tccccaaggc gggttactgc ggttcgtctt ttatttcaag cgtaccctgc
     2701 acatgggtta tatcaagcac atcaaatggt tctttagatg tcagcccggt accggttacc
     2761 acatttccac ctgtcgtgag cctgacaaac tggttggaat agatcacggc tttggcccgg
     2821 tcccagaaaa gctcttcggt attcagtttt tcttctttct ccaggttctc gaccacgacg
     2881 ttatgccggg cttccatcag tttctttttt tcataggaga ttccgtaatc agccgtgatc
     2941 ttagtggtca ggttcatggc tgtatcgtag aaaaaaacgg tgaatccttc ggggaacagg
     3001 agcagttctt catcgccttt tttattgacg aggtaagggc tgaccagttt gatctgcatt
     3061 tgtccctttt cgctgtaaag aatttcaata tcccttgctg tcatttcggg aagagtatcc
     3121 acaaaatcca gtgagagaac cgtctgaaca tcatttttac aggatgaaaa aatcgccggc
     3181 cccatcagag cgatgatgcc ggcgatgata atcaacaata ttttattctc cccaagagaa
     3241 ggcctggttt tcaaaacggg tgcaattatt gttttgacgc cctgatccgg gtatcttcat
     3301 tgatccagca ttccaccctg taaatatcac cttccttcag ggtatagaaa aagatagtag
     3361 aagtggcagg gaaataggcc gaataggtgt aaatcagttt atcggcttcc gggcccagtt
     3421 ccggctcgac ctgtttggcc cgctggtact tatcgacagc cacccaatag gccacatggt
     3481 tggtcaggtc attgcctccg caatccctgg cagaggcggc ataaaggtca ccgatcagca
     3541 tatagggatc cccgtcggtc ggatcgagag cagcggcctt caaagcatag gccctggacg
     3601 aaggatagtt gttcagcgcc tggtaagctt tggcgatata catgtagctt ttctgcactg
     3661 tagcggtatc attgagcttc tccgcctgtt taagataatc gatggcctcg gcatatttcc
     3721 cttcattcag cagcatttta ccgatcatgt aagccgattc gggtgaaggc tccaattcat
     3781 acagcttcac tgttgtttcg aaatagaaag gatcggtatg gcattttttc tgttccagca
     3841 aattgctgat cttcttaagc aattcgacat catccggagt ttgattgaat ttcttacggt
     3901 agatggtcac caggtcgggg caggtggcaa agggttcgag tttgaagtca atactggctt
     3961 taatcaatcc ccaattgccc ttatcaggtg aaatattttg ttcttgccag cttgttaaaa
     4021 ttccattctc aaaatagaga tacaaattat cataaatcca ctgttcagtt tctccatttg
     4081 aagtaactga tttgtttaca cttttaggaa cacctaaata aactctggtc tcagaatcat
     4141 ttaaacccac ttttaagttc tttgtgatac tttcagatgc cagctttttt aaattatttt
     4201 caacgatctg catagaactt tcatatgcat caaatattaa tgatgaatct gatcgctcct
     4261 tagctaggtt tattgcagca tccatataat acggtaaaat tgcacctaac gtattgtccc
     4321 cttccaattc aatagaccta tggagataat taaaaatatt ctctatatct tgcggcctat
     4381 aattaaatag atcgacacct tgtcgaccta aaacaaaacc ctcctttcca aagtatttta
     4441 tacgttggtc atataacatc atcaatgtgt caacatatct attttttaat ttatcaaaat
     4501 agttaaaagt ttctttattc tctttatatt ttagggctga atcattggtt gattcaataa
     4561 gataacttag aatcttcgcg ccatcgatat atgtattctg agttcccctg gggcagttca
     4621 aaaagaccca tctccatggc gggatcaggt cgtggatcgt ttcactttta taatcgctgg
     4681 ctttccactg tttgaaaaac tcccggtaca atgaaatgtt cgtgatgcag gtgaggctgt
     4741 cgctaccgta tttgccggct ccctggtctt cctgcccgaa actgttcaca gaagggatac
     4801 cggcgaaaat cagtgaaaag gtaataacaa aaaataggcg tttcataatt ataatgatta
     4861 ataatatttg cttttaaaga accagttttc aaaaacgttg acagcgatcg taaatttaat
     4921 aaaattttcc tggatcaacc cgttctggat ggttcctctt tttccgatag ttgctgcaag
     4981 gttcaccgtt gatttcgatt tctttatcgg taaacccatc ccaaaactta tgccaaattc
     5041 gtcgatatac tttcccttga gatagattgg ggttcttccg tactgaaaac caatgcggta
     5101 agcaactctt tggaagtaag ttccgatatt tctcgaatcc gggatatatt caccgcccac
     5161 acctacgctc cagcggttga caagtgaatc ggaattgccg ttatagcggt atttctccca
     5221 gttttgccat gtaaagtcgg cgccggccat ccattttcca tcctttccga gggtaatgcc
     5281 ccctccggta cgcatgggga gcgtgaaact tcctttctca ttatcaacgg catcgattgt
     5341 atcgtaataa agctgggctt cggtaatatc tccgaaatag gttgtcacaa gccttgaggt
     5401 cgaagatccg atctcaagtt cgggaccgaa cacgccgccg atgaccataa acagatcctt
     5461 tgaaagtttt tccctgtact ggaaaccgat ctctccataa aaatcgctcg ggcgcataga
     5521 gctgcggatg taggtatttt tcatctcgat ggaatcgggg aaagtgacgc cccggctgcg
     5581 ggtaatgttc ccgaacagat attttacatt aaatccaacc gaaagttttt taccaatctt
     5641 aaacccatta ccccagtaaa gctggttcaa tccgcccgaa ccgcggtaaa catactggat
     5701 atcgggcatg ccggtttgct gttccgtatt gaaaatatcg tatcccacat aactgaaagg
     5761 gagcacgccg aagctggtgc gccaccagcg tgtcaccggg aaaccaaaga gaagatgact
     5821 gagggaaaca tagctgccct ggtttttctg gttggtgacc tgcaggttcg acatcaaacc
     5881 gaacaccccg gcgtcgaaca caaagctgac cgaatcgaaa gcggtatagg aagcgggatt
     5941 ggccgtattg atcacactgg gcgagcggaa accggaatag agtccgccca tggcggtaat
     6001 acgcggatca ttgccgtttt ctaccagtgt acccaatccg taacgggtat acggcgagtt
     6061 gatactgatc tgaccgtata aggaggacaa ccggaaggag gcaaccagta aaagccctaa
     6121 taacaagtat ttaccctttg attgagtgat cattatagtc caaaatttcg ttcaatccca
     6181 gcagcactaa atcgggggct gcaaagatgc taatttttag tttattatgc aagaaatgat
     6241 gatcaccgcc ggtaataacc acggccaggt caccggattt ttcatggtag cggccggcaa
     6301 gatgagtgat ttcgcccgtg atgccgttat aaaccccgga aaggatagat tcctgtgttg
     6361 tttgtccgat aaggtcatca aattcttcct gttcaattaa cggcaattta ccggtgaaag
     6421 tatgcaacgc cttaaaccgc atccggattc cgggcgatat tgctccgccc aggtaagttc
     6481 cttcggcggt gatcaggtcg taggtgatgc aggtaccggc gtcgatcacc aggcaattcc
     6541 ggccgggaaa aagattgcgg gccccggcaa cggcagcgat acggtctttg ccgagtgttt
     6601 tcggtgtccg gtaaaggttt cggaacggca gcggcgtttg atcggtcagg aggataaacc
     6661 tgccggcggc ggccaggatc tcttccaatt gacgtgtatg gataatgacc gacgactgga
     6721 tgatgccatc aaaagggccg tgttgtgcaa taaaaccggt aagggaatcg ggtttcagct
     6781 tgtcgaaggt ttctttgaac agcatggtct tcccatcaaa aacagcgagt ttctgcaagg
     6841 tattcccgat gtcgataatg agttttctgt gcaagtttga tagtaaagtg tgcaaagata
     6901 gcatttttca gtcggcagtc ggcagtcggc agtcagcagt cggcagtcga ctttttttgc
     6961 ccgtgttata ttatttacca aaaaatgtat atctttgcgc tcccaatttt gctggtacca
     7021 tagctcagtt ggtagagcaa cggactgaaa atccgtgtgt cgctggttcg actcccgctg
     7081 gtaccaccgg tagcctgatt ttccagaaag gaagatcagg t
//
LOCUS       DAOPNH010000178         7092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12025_length_7092_cov_7.439676, whole genome shotgun sequence.
ACCESSION   DAOPNH010000178 DAOPNH010000000
VERSION     DAOPNH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12025_length_7092_cov_7.439676"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKZ74_09780"
     CDS             <1..202
                     /locus_tag="PKZ74_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93087.1"
                     /translation="PRILITGIGKSGSTALFYSILKALPDCTIQLFEPDNIKKTLPSD
                     TPPPALIKSFIPYSENFSFFEK"
     gene            complement(607..2013)
                     /locus_tag="PKZ74_09785"
     CDS             complement(607..2013)
                     /locus_tag="PKZ74_09785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM93088.1"
                     /translation="MYTKDDIFLYFEKLAPKRLKWKKRNRFYHSLLEKYYRFLVPEGS
                     KVLEIGCGTGDLLHAVKPTQGVGVDISPAMVGIAGDRYPGLAFYTQDAESLSLDQRFD
                     YVIMSDLLGSLHDIQQAFACLKNVTDRNSKIIITQYNFLWEPALRFLEFSGLKARQPR
                     QSWLSMHEIRKLLELEGFECFKSFRKILLPLRIPLVATFFNKFLANLPLLNHLCLVNF
                     IVARRDEPVEEELSVSIVVPARNEKGNIENAILRTPGFGRWQEYIFIEGHSSDGTYEE
                     MLRVKEKYPELRIKTAIQPGRGKADAVRLGFEMAEGDILMILDADLTTPPEDMPKFYL
                     ALSRNRGEFINGSRLVYPMEDEAMRLLNLLGNKFFSLFFSYLLDQQLKDTLCGTKVLR
                     RTGYEKIKANRKYFGDFDPFGDFDLLFGASKLNMKIVEIPVRYRNREYGSTQISRFRH
                     GWLLIRMSLFAAMKIKFL"
     gene            2036..>3311
                     /locus_tag="PKZ74_09790"
     CDS             2036..>3311
                     /locus_tag="PKZ74_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93089.1"
                     /translation="MAISKSKIIAISVFIISFVVFYAGFRYNTWQVLKNGKFLDNQAE
                     SLVVGRLVKSAADGITSSGGLCGRCYNQKNDTALFENQYRAFLNNESCKKYVPYLSQI
                     GFHAMVYFAIDKASPFKKSTDLQIMYAFKSGMLAMVMSFIVLWFLLELGLLPAIFVSA
                     GILLTPWFTFLGRDLWFCIWTNFLPFLAALFILRQENRTGKPNGRSILLIVSLVVLFS
                     FIYNGFEWVTTTLIMTAVPFFYYWRMGQWPFARLAKRIGWLVAGSLASVMITFSILIY
                     QIAQVKGTISKGIEWLIFSFQKRTYGGGEDLPEVFEKQIDHSIWEVFLINLGGVAFPF
                     PAFISQHVPRYFGGILFAEILFILIIITCLILYRRNYLKIKEENRKWLQNLSIVTWIS
                     LLAPFSWFVIFKGHAWSHHHINYITWWMPFGLF"
     assembly_gap    3312..3411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3412..4047)
                     /locus_tag="PKZ74_09795"
     CDS             complement(<3412..4047)
                     /locus_tag="PKZ74_09795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM93090.1"
                     /translation="MNIFLRIFTIPRAACHLRSLFLNLRLGFRDRFRIVVYRRTKVCI
                     SRKGKLIFDPGARLRLGLCWPMTNPNFSTLKIDEHAEMVVHGDWIFHSGIFISVNKGA
                     RLELGSGISNNDADITCFNSIRIGNHVFISKGVIIRDSDNHNVLKEGYLQSKPILIGD
                     NVWIGMRAIILKGVTIGDGAVVAAGAVVTRDVPPNTVVGGVPARVIRENVSW"
     gene            complement(4084..5001)
                     /locus_tag="PKZ74_09800"
     CDS             complement(4084..5001)
                     /locus_tag="PKZ74_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93091.1"
                     /translation="MKLGITVVYFVKKDEGWLIDLHLNYIRKNTRVPYKIYAGAERLL
                     KEYRGKLEDSGDLIICDLSTTKDIAGKENSFYLEQLFRIAIDDGCTHIATFHVDSFPL
                     GSGWADSMLNKLDQGFQLAAIERLEDNDHKPHSSFMFFSSEYYLKYHPPLRLEEEEKN
                     TDTYKNYSKRFPHIQDSGYGFGYRLYRDNLTWYPLRRSNDKQNGRLMAGVYDDIVFHL
                     GGAGFTFHISRNDKIWYWRREAVLRLLFRFMRKSGIPTGKLETRYLPGFDHSKTKTRI
                     NNRNNTIFRKVMEKLKKDPDRFLDSLRMK"
     gene            complement(4998..6281)
                     /locus_tag="PKZ74_09805"
     CDS             complement(4998..6281)
                     /locus_tag="PKZ74_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93092.1"
                     /translation="MIEELKHLDHPIRLGIVGMGAMGRGLLYQAGVTPGFRYVAVNDI
                     VPRRAEECARWLGIEYINTGRSQKPEEVIASGKLAVAADPLDVARCNQVDVFIEASSG
                     IVESALYCKTAIEHGKHVVMMNAEADLAFGPCLMKLAKEHGVVYSSCDGDQPGIIKHL
                     YDRMVFWGFEPVMAGNIKGFLDRYSDPVKIIPEADKRRLDYKMATAYTDGTKLNIEMS
                     LVANALNMRTKIPGMYGPKAKDIREVFGLFDFKKLWADRVPFVDYILGAEPGGGVFTV
                     GYCEDDYQQFMMNYYKMGDGPFYLFYRPYHLCHVESMEWIARAYLYKEALLKPDFGFK
                     TNVIAYAKKDMKKGEMLDGLGGFACYGQIENTGDYAEEGLPVCLAEKVVLQRDMLRDE
                     RIMMNDIDPAGNEMGFELHRQSVKACQEVLFPAIL"
     gene            6378..>7092
                     /locus_tag="PKZ74_09810"
     CDS             6378..>7092
                     /locus_tag="PKZ74_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93093.1"
                     /translation="MAIKQKFDPKSNYALTLEMIGYVPRKQATQADYDRIGFMSGLEV
                     HQQLLTKYKLFCRCPAGVYNKHEVYDAELIRHMRPTLSELGEYDGTALMEFKTKKEIV
                     YRIKNETACTYDVDDTPPFKIDQEALNIAIEISLLSKLNIVGEVHITRKQYLDGSIPT
                     GFQRTAIIGVEGEIPLKFKKIRLIQLSLEEDSCREMWDVGHQRGYKTDRLGMPLIETV
                     TYPDCTNPEEVREACNYIRF"
BASE COUNT         1757 a         1868 c         1585 g         1782 t
ORIGIN      
        1 tccccgcatc ctcatcaccg gcatcggcaa atcgggttca accgccctgt tctattccat
       61 cctgaaagcg ctgccggatt gtacaataca gctttttgaa ccggataaca tcaaaaagac
      121 tttaccttcc gacacgcctc ccccggcact cataaaaagt ttcatcccct attcggaaaa
      181 ctttagcttc tttgagaaat agatcctgct ggtccgcgat ccgcgcgaca acctcatcct
      241 gctgctttac aaaccctaca atatcatcag gaagttttcc acggagaaaa ggaaatagct
      301 gttcaactga tccgctcatt tcttgatagt attaagcaaa aggaaaccgg tcagcatgag
      361 atctccgtct gtagcctggc cctaaaaact acctgggact agagcttgac aacgggaccg
      421 aaatcccgtc gaattttgaa agggtggccc ggcataaaaa atactgcgat tggagaaact
      481 ggctcacccc ggaagacatt gacttttaac gtccaatgat gaaaagttac atggacaaat
      541 tcggctatgc ggatgaatgg caattggaag aacaaccaaa actggatcct tccgtttact
      601 ttcttcttaa agaaacttga tcttcattgc cgcaaaaagg ctcatccgga tcagcaacca
      661 tccgtgcctg aagcggctga tctgggtgga gccgtactcc cggttgcggt accttacggg
      721 tatctccacg atcttcatat tcagcttgct ggccccgaac aacaggtcga aatcgccaaa
      781 cggatcgaaa tcaccgaaat atttccggtt ggctttaatc ttctcgtagc ccgttctccg
      841 gagcactttg gtgccgcata gcgtgtcttt aagctgctgg tcgagcaggt aggagaaaaa
      901 caggctgaag aacttgttcc cgagcaggtt cagcaggcgc atcgcctcgt cctccatcgg
      961 gtagaccagc cggctgccat tgatgaactc accccggttc cggctgagag ccaaataaaa
     1021 cttcggcata tcctccggcg gggtggtcag gtcggcgtcg aggatcatca ggatgtcgcc
     1081 ctccgccatc tcaaaaccaa gccgtaccgc atcggctttc ccccggcccg gctggatggc
     1141 cgttttgatg cggagctccg ggtatttctc cttcacccgc agcatctcct cgtaggtgcc
     1201 atccgaagaa tgcccttcga tgaaaatata ctcctgccac cggccaaatc ccggcgtccg
     1261 gagaatggca ttttcgatgt tccctttctc gttgcgggcc ggcacgacga tcgaaacgga
     1321 aagctcctct tcgaccggct catccctcct ggccacaatg aagttgacaa ggcagagatg
     1381 gttcagcaaa gggagattgg ccaggaattt gttgaaaaac gtggcaacca ggggtatccg
     1441 aagcggcagc agtattttcc ggaaactttt aaaacattcg aacccttcga gctccagcaa
     1501 tttcctgatc tcatgcatcg atagccagct ctgcctgggt tgccgtgctt tcaacccgct
     1561 gaactccagg aaccgcagcg ccggttccca caggaaatta tattgcgtga ttatgatctt
     1621 gctgttccgg tcggtgacgt ttttcaaaca ggcaaaagcc tgctggatat catgaaggct
     1681 gcccagcaga tccgacatga tcacgtaatc gaaccgctgg tcaagcgaaa gcgattccgc
     1741 atcctgggtg taaaaagcca ggccgggata acgatccccg gctatcccga ccatagccgg
     1801 cgaaatatcg acgccgactc cctgcgtagg ttttacggca tgcagcagat cgcccgtccc
     1861 gcaacctatc tccagcactt tgctgccttc gggtaccagg aaacggtaat atttctccag
     1921 cagcgaatga taaaaacggt ttcgtttttt ccacttcagc cttttcggcg ccagcttttc
     1981 aaaataaaga aaaatgtcgt cctttgtata cataacggat taattcgtct tacccatggc
     2041 aattagcaag tcaaagataa tcgcaatatc ggttttcatc atcagctttg tcgtttttta
     2101 tgccgggttc aggtacaaca cctggcaggt actgaagaac ggaaaattcc tcgataacca
     2161 ggccgaaagc cttgtcgtgg gacggcttgt caaatcggct gccgatggca ttacctcttc
     2221 cggaggcctt tgcggccgtt gttataacca gaaaaatgac accgcgcttt tcgaaaacca
     2281 gtaccgggca tttctgaata acgaatcctg caaaaaatac gttccctatc tgtcgcaaat
     2341 cggttttcat gcgatggttt actttgccat cgataaagcc tctccgttta agaaaagcac
     2401 cgatctgcag atcatgtatg ctttcaaatc gggaatgctg gccatggtga tgagctttat
     2461 tgtcctgtgg ttcctgctgg agttggggct gctgccggct attttcgtat cagcaggcat
     2521 cctcctgacg ccctggttca ctttcctggg ccgcgacctg tggttctgca tctggaccaa
     2581 tttcctccct ttccttgccg cacttttcat cctgcgacaa gagaacagga cgggaaaacc
     2641 taacggacga tctattttgc tgattgtcag cctggttgtt ctgttcagct ttatttacaa
     2701 cggtttcgaa tgggtaacca ccacgctgat catgactgcg gtaccgtttt tctattactg
     2761 gcggatggga caatggccct ttgccaggct ggcaaagcgg atcggctggc tcgttgccgg
     2821 cagcctggct tcggtgatga tcaccttctc tattttgatc taccagattg cacaagtcaa
     2881 aggaacgatt tcgaaaggga tcgaatggct catcttttct tttcagaagc gcacttacgg
     2941 agggggtgag gatctcccgg aagtgtttga gaaacagatt gatcattcca tctgggaggt
     3001 tttcctcatc aacctgggag gtgtggcatt ccccttcccg gcattcatca gccagcacgt
     3061 tccgaggtat ttcggaggga tcttgtttgc ggagatcctc ttcattctca ttattattac
     3121 ttgtctgatc ttgtacaggc gtaattattt aaagataaaa gaagaaaaca ggaagtggtt
     3181 gcagaatctt tccattgtca cctggatatc gctcctggcg ccgttttcat ggtttgtgat
     3241 cttcaaaggc cacgcctgga gccatcatca tatcaattac atcacctggt ggatgccttt
     3301 cgggctgttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccacgaaac
     3421 attctccctg atgaccctgg cgggaacgcc acctacaacg gtgttcggag gcacgtccct
     3481 tgtcacaacg gctccggcag cgacaacggc gccatcaccg atcgttactc cttttaagat
     3541 aatcgcccgc atcccgatcc agacattgtc gccgataagg atcggtttcg attgcaaata
     3601 cccttctttc agtacgttat gattatccga atcccggata atcacccctt tggaaataaa
     3661 aacatgattt ccgatcctga tgctgttaaa acaagttata tcggcatcat tattcgaaat
     3721 tccgctacca agttccagcc gggctccttt attcacagag atgaaaatcc cggaatggaa
     3781 tatccagtcg ccatgcacaa ccatttcagc atgctcatcg atcttaaggg tcgagaagtt
     3841 cggattggtc atcggccagc acaggcctaa cctgagcctg gctcccgggt caaagatcag
     3901 tttgcccttg cgtgatatgc aaactttagt tctccggtag accacgatcc ggaaccggtc
     3961 ccggaatccg agccgcaggt tcagaaagag ggatctgagg tggcacgccg cccggggaat
     4021 tgtgaatatc cgtaagaata tattcatgtt atgcttcttt tgcgaatatt ttatttcctg
     4081 ttatcatttc attctcaggg aatcaagaaa acgatccggg tcttttttca gcttttccat
     4141 gactttcctg aagatggtgt tgtttcggtt gtttatcctt gtttttgttt tggaatgatc
     4201 aaacccgggc agataacggg tttccagctt gcctgtcggg atacctgatt ttcgcatgaa
     4261 ccggaacaat aatctcagca cagcctcccg gcgccaatac cagatcttat catttcttga
     4321 aatatggaaa gtaaatcctg cgccaccaag atgaaagacg atgtcgtcat aaacgccggc
     4381 cattaaccgg ccattctgct tgtcgttgct gcgccgcaaa ggataccacg tcaggttatc
     4441 tctataaagc cggtaaccga aaccatatcc cgaatcctga atatgcggaa atctcttgct
     4501 gtaattttta tacgtatccg tattcttctc ttcttcttcc agtctcaggg gaggatgata
     4561 tttcaaataa tattccgaag aaaaaaacat gaacgaagag tggggtttgt gatcattgtc
     4621 ttccagccgt tcaatagctg ccagttgaaa tccctggtcc agcttattca acatgctgtc
     4681 cgcccagccg cttcccaaag ggaatgaatc aacatgaaat gtggcaatat gcgtgcaccc
     4741 gtcgtcgata gcgatgcgaa ataattgttc caggtaaaaa ctgttttcct ttccggcaat
     4801 atcttttgtc gtactgagat cacaaatgat gagatcaccg gaatcttcga gcttgcccct
     4861 gtactctttc aaaagccttt cggcaccggc atagatttta tacggaaccc gggtattttt
     4921 gcggatgtaa ttaagatgaa gatcgataag ccatccctca tccttcttaa cgaaatatac
     4981 gacggtaatc ccaagtttca taatatagct ggaaatagta cctcctggca tgccttaacc
     5041 gactgccggt gcaattcaaa tcccatttca tttcctgccg ggtcaatatc attcatcata
     5101 atccgctcat ctctaagcat atcacgctgc aaaacaactt tctccgccag gcaaaccggc
     5161 aatccttcct ccgcataatc gccggtgttc tcgatctgcc cgtagcaggc aaatccgccc
     5221 aggccatcca gcatctcccc ttttttcata tctttcttgg cataggcaat cacgttcgtt
     5281 ttaaatccaa aatcgggttt gagcaacgct tctttgtaaa gataagcccg ggcgatccac
     5341 tccatcgact ctacatggca aaggtgataa ggccggtaga aaaggtagaa cgggccatct
     5401 cccattttat aataattcat catgaactgc tggtagtcgt cctcacagta acccaccgta
     5461 aaaaccccgc cacccggctc ggcgcccagg atataatcga caaacgggac ccggtcggcc
     5521 cacaattttt taaaatcaaa caggccgaag acctcccgga tatcttttgc tttcggaccg
     5581 tacattcccg ggatcttcgt tctcatattc agcgcattgg ccaccagcga catctcaatg
     5641 ttcagcttcg ttccatctgt gtaggcggtc gccattttgt agtcaagccg ccgtttatcc
     5701 gcttcgggaa taattttaac cggatcggag tagcggtcga gaaaaccttt gatgttgccg
     5761 gccatgaccg gctcaaaacc ccagaagacc attctgtcgt aaagatgctt gatgatcccg
     5821 ggctgatcgc cgtcgcaact ggagtaaaca acgccatgtt ccttggcaag cttcatcagg
     5881 caggggccaa aagccaggtc ggcctccgca ttcatcatga caacatgctt accgtgttca
     5941 atcgccgttt tgcagtataa agccgattcc acgattcctg aagaggcttc gatgaataca
     6001 tcgacctgat tgcaccgggc aacatccaga ggatcagcgg ccacagccag tttaccagaa
     6061 gcaatgacct cttccggttt ttgagaccgg ccggtattga tgtactctat accaagccaa
     6121 cgggcacatt cttccgcacg tcgcggaacg atgtcgttaa cggccacata tcggaatccc
     6181 ggtgtaaccc ctgcctggta aagcaggccc ctgcccatgg cacccatccc gactattcca
     6241 agccttatgg gatgatcgag gtgttttaat tcttcaatca tacagcaaga ctattttcag
     6301 ctaaattaga aaatagttga tacaaccatg agtgcaaatt tccttacttt tgaccccttg
     6361 ttatttgatt cacaattatg gcgattaaac agaaattcga tcctaaatcc aactacgccc
     6421 tgaccctgga aatgattggc tatgttccac ggaaacaggc tacccaggcc gattacgacc
     6481 gtatcggctt catgtcgggg ctggaggtgc accagcaact gctaacaaaa tacaaactgt
     6541 tttgccgctg ccctgccggt gtttacaaca agcacgaagt ttacgacgcc gaactgatcc
     6601 gccacatgag gcccacgctg agtgaattgg gcgaatacga cggaacggcc ctgatggagt
     6661 tcaagacgaa aaaagagatc gtctaccgga tcaagaatga aacggcctgc acctatgatg
     6721 tcgacgacac gccaccattc aagatcgacc aggaggcgct gaatatcgcc atcgaaattt
     6781 cgctcctctc aaagctgaat attgtcggtg aagtgcatat cacacgcaag caatacctcg
     6841 acggatcgat cccgacagga ttccagcgta cggccatcat cggcgtggaa ggggaaattc
     6901 cgctgaagtt caagaaaatc aggctgatcc agctcagcct cgaagaagat tcatgccgtg
     6961 agatgtggga cgtcggccat cagcgcggtt ataagaccga ccggctcggc atgccgctca
     7021 tcgaaaccgt gacctacccc gactgcacaa acccggaaga ggtcagggaa gcctgcaact
     7081 atatccgttt cc
//
LOCUS       DAOPNH010000179         7088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12040_length_7088_cov_7.552680, whole genome shotgun sequence.
ACCESSION   DAOPNH010000179 DAOPNH010000000
VERSION     DAOPNH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_7088_cov_7.552680"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKZ74_09815"
     CDS             <1..673
                     /locus_tag="PKZ74_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM93094.1"
                     /translation="LIGAYIEGIKDGPRFFDAFFFSVTSSDGGVYTLLATDRVGCTDQ
                     KSIELIVSDNPVAAFHGTDTLEMHTGELLNAGAGMASYKWSTGDTTESIIIPSVGMYS
                     VEMESPVGCLGSDSVYVKLVSEEIPEFEIYVPNAFSPNGDGINDIFQIKFPHSTFNLQ
                     HLTLSIFDRWGGEVFSGEGVSSGWDGRKNGKDCPGGVYVYKIVFSLDGVPGSEERVGT
                     VMLVR"
     gene            859..2163
                     /locus_tag="PKZ74_09820"
     CDS             859..2163
                     /locus_tag="PKZ74_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM93095.1"
                     /translation="MNRLFTQKLETWKDTIGRKPLIIRGARQVGKSFTVASFGKNNFN
                     GRIHTVDFEKHPDWHGIFEKNLDPHRIVSELEILLNAKITAGTDLLFFDEIQAAPKAI
                     MALRYFYEEMPGLHVIAAGSLLEFAMKDISFPVGRVSLMNMSPMNFYEYLKACQKDKL
                     AEIVLSEPEKLPGTIHQALLDNLREYMYVGGMPQCVNVWHVNRSMAEVFDIQNDLAGT
                     YRQDFSKYAPYVDKRSLNQVLGSVASNIGHQVIYTRMSVEFTGPTNKKALDLLTLARV
                     VSKVNSASPASLPLAATSSDKRFKAILVDTGILRALNQLPANIEFMKNDLLQMYHGAL
                     AEQFAGQEFISSGQENLFYWSREAKSSSAEVDYLVVKNGSICPVEIKGGAAGKLRSLH
                     VLLKEYPKIERGYVLSIAPFGEIPSQKIVFLPLYYAYGLLRQ"
     gene            complement(2220..3209)
                     /locus_tag="PKZ74_09825"
     CDS             complement(2220..3209)
                     /locus_tag="PKZ74_09825"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPM93096.1"
                     /translation="MQKFRAAVTGIHGWVPDYILTNQELEQMVDTTDEWIMTRTGIKE
                     RRIMKEPGKGTSDIAVPAVNGLLEKTGTNPEDVELLICATVTPDYAFPATANIISDKC
                     GLKNAWSFDINAACSGFLYALHTAAQFIESGTYKKIIVVGAEKMSMMVDYTDRQTCVI
                     FGDGAGAVLLEPTAEETGVMDSIMKTDGSGRVYLYQKGGGSVKPASQETIDNREHYVY
                     QEGQAVFKFAVVGMADVSAEIMERNNLKSEDIAYLVPHQANLRIIDATARRMGLTKDK
                     VMINIEKYGNTTSATIPLCLWEWENRLKKGDNLILSAFGGGFTWGAIWLKWAY"
     gene            complement(3218..4180)
                     /gene="plsX"
                     /locus_tag="PKZ74_09830"
     CDS             complement(3218..4180)
                     /gene="plsX"
                     /locus_tag="PKZ74_09830"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPM93097.1"
                     /translation="MNIGLDVMGGDFAPKATVKGACLALHEISSADKIFLIGPEEVIL
                     EELAKEGVDPGRFVIINAPEVIGMGEHPTRAVVQKPNSSISVGFHLLKHGKIDAFSSA
                     GNSGAMVVGSMYSVNTIQGIIRPCTSAVVPKENGTVSILLDVGTITDVKPDVMYQFGI
                     LGSLYAEHIFKIPNPRVGLLNLGAEEEKGNLQCQSSFALMKNSKDFNFIGNVEGRDIF
                     KDKADVIVCDGFTGNVVLKLMESFYRILVKRNLRDEYIDRMNYENYGGTPLLGINSSV
                     IVGHGISNDIAIKNMILLSKEVHEVELSIKIQNALQKFSLNNKE"
     gene            complement(4185..4373)
                     /gene="rpmF"
                     /locus_tag="PKZ74_09835"
     CDS             complement(4185..4373)
                     /gene="rpmF"
                     /locus_tag="PKZ74_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPM93098.1"
                     /translation="MPHPKRKHSKTRRDKRRTHDKIEKPALAKCPVTGTVHVYHRAYY
                     VDGDLYYKGKLVVSKAGE"
     gene            complement(4385..4918)
                     /locus_tag="PKZ74_09840"
     CDS             complement(4385..4918)
                     /locus_tag="PKZ74_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPM93099.1"
                     /translation="MNYLRQYIIPFSGLKAGNYDYSFEIDGRFFEEFEYSEIREGKLH
                     VDCLLEKQDRMMIFSFRIKGAVRVICDRCAGEFDWPVEGKQQLIVKFGQEHTEEAEDI
                     LVITEKEHELDLSQYLYEYIHLLVPIRKIHGTDENGNSLCDPDVIRYIKEEEEHPADP
                     RWDILKKLKDNKKDDNN"
     gene            5101..6012
                     /locus_tag="PKZ74_09845"
     CDS             5101..6012
                     /locus_tag="PKZ74_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPM93100.1"
                     /translation="MFNRMIAGLLPYMPKSFVWIFSKRYIAGKKIEDAIRVSRELNGQ
                     GIKVTVDLLGEFITRLSEAEKNKIEYLDIIDTFTKENIDGNFSLKPTMFGLLIDPRVC
                     YENIREIVARAASYNNFIRIDMEDSQCVDLEIDLYRKLKAEFPKNVGLVVQAYLKRTM
                     SDLENLMDIHSKEIPLNYRLCKGIYVEPAEIAYKKYDEINSHFLEDLEFMLKNGVYPG
                     IATHDKPLVDGAYKLIEKYNVPKNMYEFQMLYGVTPELRKSIVDKGHTMRVYVPFGEQ
                     WFAYSTRRLKENPKMAWLIIKAIFVRG"
     gene            6116..>7088
                     /locus_tag="PKZ74_09850"
     CDS             6116..>7088
                     /locus_tag="PKZ74_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPM93101.1"
                     /translation="MENNMTGKIAQIIGPVVDVSFEDEAHLPNIYEALEVTNDKGEVV
                     VLECQQDIGENTVRTVAMDSTDGMRRGMEVVRTDKPISMPATEEIRGRLFNVIGKPID
                     GLGEVKGSKQYPIHREPPTFENLTTRQEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVIIMELINNIAKVYSGMSVFGGVGERTREGNDLLREMIESGVIQYGDEFLKDMEQG
                     GWDLSKVDREKLKDSNITLVFGQMNEPPGARARVALSGLTLAEYFRDGDEKSGGRDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTK"
BASE COUNT         1818 a         1676 c         1705 g         1889 t
ORIGIN      
        1 cctgattggc gcttacatcg aagggataaa ggacggcccg cgcttcttcg atgctttctt
       61 tttctctgtt acctcttcag acggtggggt atataccctg ttggccaccg accgcgtcgg
      121 ctgcaccgac cagaaaagca tcgagctgat cgtcagcgat aatcccgtag cggctttcca
      181 tggcaccgat acgctggaaa tgcacacggg cgaattactg aatgcaggag cagggatggc
      241 ttcgtataaa tggagcacag gtgacacaac agagagtata attattccgt ctgtaggaat
      301 gtactccgtt gagatggagt ctcccgttgg ttgtttgggt tccgattcgg tttatgtaaa
      361 actggtttcg gaagaaatac cggagtttga gatttacgta ccaaacgcct tcagtcccaa
      421 tggcgatggc attaacgaca tctttcaaat aaaattcccg cattcaacat tcaaccttca
      481 acatttaaca ttaagcattt ttgatcggtg gggaggagaa gtgttttccg gagaaggagt
      541 ctcatcgggc tgggatggca ggaagaacgg aaaagactgt ccgggtggtg tttatgttta
      601 caagattgta ttttcattgg atggtgttcc cggcagcgag gaaagggtag gaacggtgat
      661 gctggtgaga taatccgttc actcttcacc gctcacgctt cacgcttcac gcctcacgct
      721 tcacgcctca cgttttggac ggtaatgctg gtgcggtaat agtacaaaat tcaaaattaa
      781 gattcgtttt caagaataag ccttgttatt ctaaataaca tctattatat ttgccttgtt
      841 atactaaatt cttttgaaat gaacagactg ttcacgcaaa agcttgaaac ctggaaggac
      901 acgattggaa gaaaaccgct gatcatcagg ggagcgagac aggttggaaa gtcatttact
      961 gtcgcatcgt tcgggaagaa caattttaat ggtagaattc atactgttga ttttgaaaaa
     1021 catcccgact ggcatgggat atttgagaaa aacctggatc ctcacaggat agtaagtgag
     1081 ctggaaatac ttcttaatgc aaagattacc gctggaacag acctgctttt ttttgatgaa
     1141 atccaggctg ccccgaaagc gattatggct ttgaggtatt tttatgaaga aatgccaggt
     1201 ttgcatgtga ttgcagccgg gtcgctgctc gagtttgcca tgaaggacat cagttttcct
     1261 gtgggcaggg tcagcctgat gaatatgtcg ccgatgaatt tttacgaata cttgaaggca
     1321 tgccagaaag ataagctggc ggaaatagtc ctttccgaac ctgaaaaact gccgggcacg
     1381 atccaccagg cattactgga taatttacgg gaatacatgt atgtcggggg gatgccacag
     1441 tgtgtcaatg tctggcatgt gaacagaagc atggcggagg tctttgatat tcagaatgat
     1501 cttgcaggga cttaccggca ggatttttca aaatatgcgc cttatgtgga taagcgaagc
     1561 ttgaaccagg tgcttggttc tgtagcttca aatattggcc atcaggtcat atataccagg
     1621 atgtccgttg aattcaccgg tcctaccaat aaaaaagccc ttgatttact tacccttgca
     1681 agagttgtga gcaaagtcaa ttctgcttct ccggcatcct tgcctctcgc tgccacatca
     1741 tctgataaaa gattcaaggc catactggtg gataccggga tcctccgtgc attgaaccaa
     1801 ttgccggcaa atattgagtt tatgaagaat gatttgctgc agatgtatca tggcgcattg
     1861 gcagaacaat ttgccggtca ggagtttatt tcttccggcc aggagaattt attttactgg
     1921 tcacgggagg ctaaaagcag ctcggcagag gttgattatc tggtcgtgaa aaacggcagc
     1981 atctgtccgg tggaaatcaa aggcggggca gccggcaagc tccgcagcct gcatgtgctg
     2041 ttaaaagaat acccgaaaat tgaacgtgga tatgtgcttt ctatcgctcc gttcggtgaa
     2101 ataccctcac agaagatcgt ttttctgccg ttgtattatg catacgggct tcttcggcaa
     2161 taaaactaat tttagaaggg ataaagtcga atatcgcctg tttgtctgat cggcctatct
     2221 cagtaggccc acttcagcca gatggcgccc caggtgaaac cgccgccgaa tgcggaaagg
     2281 atcaggttgt cgcctttctt tagccggttt tcccattccc agagacacag cgggatagtt
     2341 gccgaagttg tattgccata tttctcgatg ttgatcatca ccttgtcttt ggtgagaccc
     2401 atccggcggg cagtggcgtc gataatgcgg aggttggcct ggtggggaac caggtaggca
     2461 atatcttccg atttcaggtt gttccgctcc atgatctccg ccgaaacatc agccatgccg
     2521 actacggcaa atttaaagac cgcctgtcct tcctggtaaa cataatgttc ccggttgtcg
     2581 atcgtctcct gcgatgccgg tttcaccgat ccgccgcctt tctggtagag atacacgcgg
     2641 cccgagccat cggtcttcat gatactgtcc attacgcctg tctcttcggc agtgggttcc
     2701 agcaacactg cgccagcccc gtcgccgaag atcacgcagg tttgccggtc ggtataatcg
     2761 accatcatcg acattttctc ggcgccgaca acaatgatct tcttgtaggt ccccgattcg
     2821 atgaactggg ccgcagtatg caacgcatat aagaatccgg agcatgccgc attgatgtcg
     2881 aagctccagg cattctttaa cccgcatttg tcggaaatga tattggctgt ggcagggaat
     2941 gcataatcgg gagtaaccgt agcgcatatc agcagttcta catcttccgg atttgtgcct
     3001 gttttttcca ggagtccgtt cacagccggc acagcaatat cggaggtgcc tttgcccggc
     3061 tctttcatga tccttctctc cttgataccg gttcgtgtca tgatccactc atccgttgta
     3121 tcaaccatct gttccagctc ctggtttgtc agtatatagt caggtaccca accgtggata
     3181 ccggtaacgg cagctctaaa tttctgcatg taatcgatta ttctttgtta ttcagtgaaa
     3241 acttttgtaa cgcattctgg attttaatgg aaagctccac ttcgtgcacc tctttcgaca
     3301 gaaggatcat gttctttatg gcaatgtcgt tggaaatacc atgcccaacg ataacagaag
     3361 aattaattcc cagtaatggc gttccgccat aattttcgta gttcatccgg tcaatgtatt
     3421 catcccgcag gtttcgcttc acaaggatac ggtaaaatga ctccatcagc ttcagtacta
     3481 catttcccgt aaaaccgtcg caaacgatca catccgcttt atctttgaaa atatcccttc
     3541 cttcaacatt gccgataaaa ttgaaatctt tggaattttt catcagagca aaggaagact
     3601 ggcactgcag gttccccttc tcctcttcgg ctcctaaatt caaaaggccg acacgcgggt
     3661 tcgggatttt gaagatatgc tcggcataaa gcgatccgag gataccgaac tggtacatga
     3721 catccggttt gacatcggta atggtgccga catccaacag gatggaaacc gttccgtttt
     3781 ctttgggcac cacggctgaa gtgcagggcc tgatgatgcc ctgtatcgtg ttgacgctgt
     3841 acatcgaacc gaccaccatg gcgccgctgt tgccggcact gctgaatgcg tcgatcttac
     3901 cgtgtttcag caggtgaaac ccgacggaga tactggaatt aggcttctgg acaaccgcac
     3961 gggtgggatg ttctcccatt ccgatcactt ccggggcatt gatgatcacg aaccggccgg
     4021 gatcgacacc ttctttggcc aactcttcaa gaatgacctc ttccggtccg atcaggaaaa
     4081 ttttatcggc ggaagagatt tcatgaagtg ccaggcaagc acctttaaca gtagctttcg
     4141 gggcaaaatc cccgcccatg acatcaagcc cgatattcat ctaattattc ccctgctttg
     4201 ctaacaacca gctttccttt gtagtaaagg tcgccgtcga catagtaggc gcgatgatat
     4261 acgtggaccg tacccgttac cggacatttg gccagtgcag gcttttcaat cttgtcgtgc
     4321 gttcttctct tatcccgtct ggtttttgaa tgttttcttt ttgggtgtgg cattgttgca
     4381 agatttaatt attgtcgtct tttttattgt ctttcaattt tttcaaaata tcccagcgcg
     4441 gatctgccgg gtgttcttct tcctctttga tataccggat gacatcaggg tcacagaggc
     4501 tgttgccgtt ttcatccgtg ccgtgaatct ttcttatcgg aaccagcaaa tgaatatatt
     4561 cgtacaggta ctggctcaga tccagctcat gctccttttc ggtgatgacc agaatgtctt
     4621 ctgcctcttc cgtatgctcc tgcccgaatt tcacgatcag ttgctgcttg ccctccacag
     4681 gccagtcaaa ttcccccgca caacggtcgc aaattactct tactgcccct ttaatcctga
     4741 aactaaagat catcatcctg tcctgctttt caagcagaca atctacatgt aatttccctt
     4801 ccctgatttc cgagtattca aactcttcaa agaacctgcc gtcaatttca aaggaatagt
     4861 cgtaatttcc cgcctttaat ccgctgaaag ggatgatata ttgcctcagg taattcacgt
     4921 atatccctcc ttaaaaattg gggtgcaaat ttagggatat tattttgaat aacaaaagaa
     4981 taatttgaaa tccaaaattt gcggcacttt tgttaaagaa ttaacaaaag attaattttg
     5041 caccgtttca atttcgaaat cgtgcattta aagtgtttta aattataaat ttctaaagag
     5101 atgtttaaca ggatgatcgc cggcctgctg ccgtatatgc caaaaagctt tgtctggatc
     5161 ttttccaaaa gatatattgc cggcaaaaaa attgaagatg caatccgcgt ttcgcgggaa
     5221 ctgaacggcc agggaattaa agtcacggtc gacctcctcg gggagttcat cacccggctc
     5281 agtgaagccg aaaagaataa aatagagtac ctggatatta tcgatacgtt tacgaaggaa
     5341 aatattgacg gtaatttttc cttgaaaccg accatgttcg gattactgat cgatccccgg
     5401 gtttgctacg aaaatatccg ggaaatagtt gccagggctg catcttacaa taattttatc
     5461 cggatcgaca tggaggactc acagtgtgtc gacctggaga tagaccttta ccggaagctg
     5521 aaagccgaat ttcccaagaa tgtcggcctg gtggtacagg cttacctgaa gcggacgatg
     5581 agcgatcttg aaaacctgat ggatatccac tcgaaagaga tcccgctgaa ttacaggctc
     5641 tgtaagggga tttacgttga accggcagag attgcctaca agaagtatga cgaaatcaac
     5701 agccatttcc tggaagacct ggaatttatg ctgaaaaatg gcgtttaccc cggcattgcc
     5761 actcacgata aaccgcttgt ggacggcgca tacaagctca tcgaaaaata taatgtaccc
     5821 aaaaacatgt atgagttcca gatgctatac ggcgtcacac cggagctgag gaaatccatc
     5881 gttgacaaag gacacacgat gcgggtttat gttccgttcg gcgaacaatg gtttgcttac
     5941 agcacccgcc gcctgaagga aaatccgaaa atggcctggc tgatcatcaa ggcgattttt
     6001 gtgagaggat aacgataatg tagagacgca acatgtgtta tgtcgtaaag acgcaaaata
     6061 ttgcgtcttt acgataatgt attatctttg ccacgaaaaa tcacaaagct atcccatgga
     6121 aaacaatatg accggtaaga tcgcccagat tatcgggccg gttgtcgacg tttcttttga
     6181 agatgaagct catcttccga atatttacga agcccttgaa gtaaccaacg ataagggtga
     6241 agtggtggtg ctggaatgcc agcaggacat cggtgaaaac accgtccgga ccgttgcgat
     6301 ggattccacc gacgggatgc gccgcggaat ggaagtcgtc aggaccgata aaccgatctc
     6361 catgccggca acggaagaga tccgcggccg tttgttcaac gtgatcggca aaccgatcga
     6421 tggcctcgga gaggtcaaag gatcaaagca gtacccgatc caccgcgaac cgcctacctt
     6481 tgagaacctt accacccgcc aggaagtact ttataccggc ataaaggtga tcgacctgat
     6541 cgagccctac tccaaaggag gcaaaatcgg gttgttcggt ggcgccggtg tgggcaagac
     6601 cgtcatcatc atggagctca tcaacaacat cgcgaaagtt tattccggta tgtcggtgtt
     6661 cggcggtgtc ggcgaacgta cgcgcgaggg caacgacctg cttcgcgaaa tgatcgagtc
     6721 gggcgttatc cagtatgggg atgagttttt gaaagatatg gaacaaggcg gctgggacct
     6781 ttcgaaagtc gaccgcgaaa agctgaagga ttccaacatc accctggtat tcggacagat
     6841 gaacgaacct cccggagccc gcgcccgcgt ggcattatcg gggctcacgc tggcggagta
     6901 tttccgcgat ggcgacgaaa aatcgggcgg ccgcgatatc ctgttcttta tcgacaatat
     6961 tttccgtttc acccaggccg gatccgaagt gtcggccctc cttggccgaa tgccttcggc
     7021 agtgggttac cagccgacgc tggctaccga aatgggtatc atgcaggaac gcatcacctc
     7081 caccaagc
//
LOCUS       DAOPNH010000180         7084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12051_length_7084_cov_4.867264, whole genome shotgun sequence.
ACCESSION   DAOPNH010000180 DAOPNH010000000
VERSION     DAOPNH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12051_length_7084_cov_4.867264"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKZ74_09855"
     CDS             <1..909
                     /locus_tag="PKZ74_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93102.1"
                     /translation="GLAEKGDHIITTMLEHNSVLRPLHHLETEGIIEVTHIPFDSHGY
                     VDPDDIRKAIRQNTKMVVVNHSSNVIGTVQPVAGIGKICRQHGVFLIVDASQSAGIIP
                     IDMKAMNIDALAFTGHKCLMGPTGIGGSYVMEHVPVRYTRFGGTGVRSAQKTHLEEFP
                     YRLECGTLNIAGVAGLHAGVNWVLAKGIENLHRMEITLWDKLRKGLQQIPGVTTYCAE
                     NPINQNAVLSFNIQGFDSGDVGTMLDVDYNIACRTGLQCAPLVHTGLGTDKIHGTVRL
                     SIGAFNTEEHVDKAIEAVADIASLRN"
     gene            complement(938..1228)
                     /locus_tag="PKZ74_09860"
     CDS             complement(938..1228)
                     /locus_tag="PKZ74_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPM93103.1"
                     /translation="MNIFIAKLSANTTSEDLRTLFSEYGEVVSANVIFDKMTGRSKRF
                     GFVEMKNDDEANKAIEELNEAEYDHSQIVVKKARPAGEGGPRPQQRRDFRRY"
     gene            complement(1386..3092)
                     /locus_tag="PKZ74_09865"
     CDS             complement(1386..3092)
                     /locus_tag="PKZ74_09865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPM93104.1"
                     /translation="MQKEKSVIIFIAFIIIWLIINSFQAGLTGLLHDEAYYFLYSEFP
                     AWGYYDHPPLTAMMIKAGTWLFHGEFGLRVLFVLLSAGTIGILYLQAKPTNIFFFALL
                     FFSFLFFQITGFLALPDSAMIFFTALFFLVYKKFTEKYDIRSALLLGTVMAGLFYSKY
                     LGIVVIFFTVLSNLNLFRKGSFWLAVAITTILLLPHLLWQYHHDFPTFYYHLTERSHD
                     EYFRWTNFLDYLVGQAGLINPILFIPVIWSLFRFKPGNTYEKALKFSAAGSLLLPFLL
                     MLKGRVEANWTMAGLLPLFLVTFIEWGKNRKMVRFATVAGLVTILLIISARVFLMADF
                     LPEKYTRMVRLEMIGWKTFSKEVEKIAGDRPVVFIGGYQIPSLYRFYTGKEAFSFNNA
                     LYRNNQYDLEDIEAKLQGRKVLLLFPRNTISPSDLVTYGYDSIMNPNGRKILYTSVDD
                     FRSYNWLRAEIIPNDNSFKAGTEQEFTVILNRPVNGPLDFSDVKTGEVFLTYLLLQQG
                     KPVQYRLFENISQLGSENNYQTSFRMTMPEKPGIYYMKVSLKTGILPPGINSRLVKIK
                     VE"
     gene            complement(3152..6280)
                     /locus_tag="PKZ74_09870"
     CDS             complement(3152..6280)
                     /locus_tag="PKZ74_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM93105.1"
                     /translation="MALKRLLIISTVLLVCQSSMGQKQANYWYFGVNAGLSFAMGAPV
                     PLTNGALNTGEGCSSISTSSGNLEFYTDGRFVYNREHDQMPHGSGLLGHSSSTQSGII
                     VPKPGSTTQYYIFTVDAKDNGLAAGLCYSRVDMTLEGGLGDVVTTEKNISLLPYACEK
                     VTAVGHSNGISIWVITHLWNSDAYYAFEVTTNGVNVTPVISHTGPAITGDMEASKGYI
                     KVSPDGSKIGIANNTAFHVSISNFSNSSGTVSHLVTDYNYVNPGGSDPGGPYGVEFSP
                     NSHFLYIGEWKANKRIWQYDVSTNDPDEILDSKVMVGSVAQDADPIGALQLGPDNRMY
                     IARNGSAYLSRINSPNVAGTGCGFTDNAVNLAGRQSRYGLPPFIQSFFYLSADFYWDV
                     PSCDGTPVQFYTSASDNPDSVKWNFGDPGSGAENQSTLVNPTHLFTNTGNYWVTLNVY
                     LYGVMKNAFHIVVVREQPEVYIGNDTTLCAYEPFYLDAGSGFEHYLWSNGDTTQISEI
                     TTSGTHWCKVTGEGGCTDTDYINVTLNPVPDVYAGDDQTIANGTATTILASLTGGSGD
                     FTYDWSPAALLVNPHVLQPTTVNLTGTTLFTLTVTDNAGGCVNSDQVLINVLGGALGC
                     FPYADPEGICTGNESQLHAMPTGGSGSYIYTWTSTPSGFNSDLANPFVNPSVTTTYHL
                     SLYDGYSTVNGNVTLPVYAHPLPDAGTDQSIPYGTSTTLHGYAGSGSGSYSYHWEPAG
                     SLVNPNVQHPTTTLLEVTTMFSLTVTDTETGCISEDPDVTIVTVTGGPLAVQPQALPD
                     TICSGETTQLFAMASGGADWQYTYNWSSNPPGFSNTTSSPFVTPTVTTTYTVQLDDGY
                     KQVVGSVTVIVNPTPAVNLGPDLTICVFDTVRLDAGYWGAGTKYLWSNGDTVRTIPIT
                     TTGIGFDLKNIWVTVTSPAGCVTTDNRLIIFDFAACNGIDDPSSDNGFSIYPNPGNGT
                     LRLETTGKHDICHVTVADAYGRTIIRDHEIRFSSSNPSSTIDLTGYSPGIYLVRITEN
                     GRELAYLKYLLK"
     gene            6549..>7084
                     /locus_tag="PKZ74_09875"
     CDS             6549..>7084
                     /locus_tag="PKZ74_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93106.1"
                     /translation="MRKLLLFISALFLIHMSYGQLTGIKSIPGDYATIEAAITALNAQ
                     GVGAGGVTFNVAAGHTETFTTVTAGLVTASGTAGNQIIFQKSGAGANPLITAATPGTG
                     TMDYIICLSGADYVTFDGINVQENAANTTSAMQMEWAYAVLKASVDNGSQNNTIKNCA
                     ISLNSDNTASYAVYSNNHT"
BASE COUNT         1893 a         1582 c         1801 g         1808 t
ORIGIN      
        1 ggtttggcgg agaagggcga tcatattatc actaccatgc ttgagcataa ctcggttctc
       61 cgtccgcttc accatttgga aacggagggg atcattgaag tgacccatat tcctttcgat
      121 tcccacgggt atgttgatcc tgatgatatc cggaaagcca ttcggcaaaa cactaagatg
      181 gttgtggtca accatagctc aaatgtgatc ggtacggttc agccggttgc cgggattggg
      241 aaaatttgca ggcagcacgg cgtattcctg atagttgatg ccagccagag cgcgggtatc
      301 attccaatcg atatgaaagc gatgaacatc gatgcgcttg ctttcaccgg ccataaatgc
      361 ctgatgggtc cgaccggaat cggcggatct tatgtgatgg agcatgtgcc ggtgaggtat
      421 acacggtttg gagggaccgg tgtcaggtcg gctcaaaaaa ctcacctgga ggagtttcct
      481 taccgcctgg agtgtggcac attaaatatc gcaggtgtag ccgggctgca tgccggcgta
      541 aattgggtgc tggcgaaagg cattgaaaac ctgcaccgga tggagatcac tttgtgggat
      601 aaattgagaa agggtcttca gcaaattccg ggcgtaacga cctattgtgc tgaaaaccct
      661 ataaatcaaa atgctgtatt aagttttaat atccagggct ttgattccgg cgatgtggga
      721 accatgctgg atgtggacta caatattgcc tgccggaccg gtttgcagtg tgcccctctg
      781 gtacatacag gcttggggac ggataagatt cacggtacgg taagattgag tatcggtgca
      841 ttcaatacgg aagaacacgt cgataaagct atcgaagcgg tagcggatat agcatcgctg
      901 cgaaattaag cccggcgacg caccgtaaaa tcaggaatta ataccttctg aagtcgcgcc
      961 tttgctgagg acgtggtccg ccttctccgg ccggccttgc tttcttcacc acgatctggc
     1021 tgtgatcgta ttcggcttca ttcagctctt cgatagcttt gttggcctca tcatcgtttt
     1081 tcatctcaac gaatccgaat cgtttggaac gacccgtcat cttgtcaaag atcacattgg
     1141 cggaaaccac ttctccatat tcggaaaaca acgtccttag gtcttcgctg gtggtgttag
     1201 cgctgagctt tgcaataaaa atgttcatgt ggaataataa ataaaagatt aataatagag
     1261 aaaatttaag aggtttaaaa tgtctgggtg atttctggga gggttatgtt ttttgactgt
     1321 gattaattgt ttagtacaaa gataaactga aaatgaccca aatgaataac taacaaaaaa
     1381 aaaatttatt ccacctttat ttttaccagc cggctgttga tcccgggcgg gagaataccc
     1441 gttttcagcg acaccttcat gtaatagatt ccgggttttt cgggcattgt catcctgaat
     1501 gatgtttggt aattgttttc tgatcccagt tgggagatgt tctcaaagag ccggtactgg
     1561 acaggttttc cttgctgtaa aagcaggtac gtgagaaaca cttcacccgt tttgacatct
     1621 gaaaaatcca aagggccgtt aacggggcga tttagaatta ccgtgaactc ctgttctgtc
     1681 cctgctttaa aactgttatc attcggaata atttctgccc tgagccagtt ataagacctg
     1741 aagtcatcaa cagatgtata aaggattttc cggccattcg ggttcataat gctgtcatat
     1801 ccgtatgtca cgagatccga cggagatatg gtgttccggg gaaacagcag gagaaccttg
     1861 cgtccctgca actttgcttc tatatcctca agatcatatt ggttgttgcg atatagtgcg
     1921 ttattgaagg aaaacgcctc cttacctgta tagaacctgt aaagcgaggg tatctggtag
     1981 ccaccgataa ataccaccgg acggtcgccg gcaatctttt ccacttcttt cgagaacgtc
     2041 ttccacccga tcatctccag cctgaccatc cgggtgtact tttcaggaag aaaatccgcc
     2101 atcaggaaaa cgcgtgccga aataatcaat aaaatggtca ccagtccggc cacggtggca
     2161 aagcggacca ttttccggtt tttcccccat tctataaaag ttaccaggaa caaaggcagg
     2221 agtccggcca tggtccagtt agcttcaacc cgtcccttta acattaggag gaaaggcagc
     2281 aggagactgc ccgcagccga gaatttcagg gctttttcat aagtgtttcc tggtttgaac
     2341 cggaacaggg accaaatgac cgggatgaac aggatgggat tgatcagtcc tgcctggccc
     2401 accagataat cgaggaaatt tgtccagcgg aaatattcat catggcttct ttcggtgaga
     2461 tgatagtaaa atgtcggaaa gtcgtggtga tactgccaca acaaatgggg cagaagcagg
     2521 atggttgtga tagccaccgc cagccagaaa gaacctttcc ggaaaaggtt aaggtttgat
     2581 agcactgtga aaaagatcac cacgatgccc aggtatttac tgtagaacaa tccggccatg
     2641 accgtgccca gcagcaaagc ggaccggatg tcatactttt cggtaaattt cttgtatacc
     2701 aggaaaaaaa gggcagtgaa aaaaatcatt gccgagtcgg gtaaagccag gaagcccgtt
     2761 atctgaaaaa ataaaaaaga gaagaacaat agggcaaaaa agaagatgtt ggtcggtttt
     2821 gcctggagat aaaggatgcc gattgtccct gccgataaaa gaacgaacaa taccctcaga
     2881 ccgaattcac cgtggaaaag ccatgtaccg gctttgatca tcatagcggt caaaggcgga
     2941 tggtcgtaat atccccaggc tggaaattcc gaataaagga aatagtaggc ttcatcatgg
     3001 aggaggcctg ttagtccggc ctggaaagaa ttaatgatca gccatataat aataaaggca
     3061 ataaaaatta tgaccgattt ttctttttgc atcagacctc atccattgtt aggagatacc
     3121 ggattaatga accactttct ccccggatta cttatttaag aagatatttc agataggcca
     3181 gttcccggcc gttttcagtt atcctgacca ggtagatccc cggggagtac cctgtaaggt
     3241 ctatggttga cgacgggttg gatgacgaaa acctgatttc gtggtcacgg atgatagttc
     3301 tgccgtaagc atcggccaca gtaacgtggc agatgtcatg ctttccggtg gtttccagcc
     3361 ggagggttcc attgccgggg ttgggatata ttgagaatcc gttgtcggat gacggatcat
     3421 caataccgtt gcaggcagcg aaatcaaaaa tgatgagccg gttatccgta gtgacacacc
     3481 cggcgggtga ggttaccgtc acccaaatgt ttttaaggtc aaaaccgata ccggtggtgg
     3541 taatagggat agttctgacc gtatcgccgt ttgaccagag gtatttggtg cctgcgcccc
     3601 agtagccggc atccagtcga acagtgtcaa atacgcatat agtcaggtcg gggcccagat
     3661 taacggccgg tgtcggattt acgataacag ttaccgatcc gacaacctgc ttatatccgt
     3721 cgtcaagttg tacggtatac gttgtggtga cggtgggcgt tacaaacggg ctggaagtgg
     3781 tattgctgaa tccgggagga ttggaactcc agttgtaagt atattgccaa tcggcaccac
     3841 cactggccat agcgaacaat tgggtggttt caccggaaca aatggtatcg ggcagcgcct
     3901 gtggttgaac cgccagaggc cccccggtta ccgtcactat cgttacatcg gggtcttcgc
     3961 tgatacaacc ggtttccgta tcggttactg tcaatgaaaa catggtcgtt acttccagta
     4021 aagtcgttgt tggatgctgg acattcgggt ttactaaact accggccggt tcccagtgat
     4081 aggaatagga tcccgaaccc gaacccgcat acccgtgaag agtcgtggat gtgccgtaag
     4141 gaatagactg gtctgttccc gcgtcgggca aaggatgggc gtatacgggc agggtgacgt
     4201 ttccgttgac ggtactgtac ccgtcataaa ggctgaggtg gtaggtagtg gtgacagaag
     4261 gattcacgaa tggatttgcc aggtcggaat tgaacccgga aggggttgat gtccaggtat
     4321 agatataact gccggagcct ccggtgggca tagcatgaag ctggctttcg ttaccggtgc
     4381 agattccttc cgggtcggcg tatggaaagc accccagggc gccgccgagc acattgatca
     4441 gcacctggtc ggagttcacg catcccccgg cattatcggt aacggtgaga gtgaagagcg
     4501 tagtgccggt aagattgacg gtggtcggtt gaagcacgtg cgggttgacc agcagggcgg
     4561 caggtgacca gtcgtaggta aaatcacctg agcctcctgt cagcgaagcc aatatggtgg
     4621 ttgccgttcc gttggcgatg gtctggtcat caccggcgta cacatccggt accggattca
     4681 gggtcacatt aatgtagtcg gtatcggtac atcctccttc gccggtaacc ttgcaccaat
     4741 gggttcctga agtggtaatt tcagatatct gtgttgtatc tccattgctc catagataat
     4801 gttcaaaacc ggaaccggca tccaggtaaa atggctcata agcgcagagg gtagtatcgt
     4861 tcccgatata tacttcgggt tgttcacgga ccacaacaat atggaaggca tttttcatca
     4921 ccccgtacag ataaacattg agggtaaccc aataattacc cgtattggta aacaggtgcg
     4981 tagggttgac cagagtcgac tgattttcag cacctgaacc cgggtcgccg aaattccatt
     5041 taacggagtc gggattatcg gaggcgctgg tataaaactg gaccggtgtt ccgtcgcagg
     5101 aagggacatc ccagtaaaag tcggcggaaa ggtagaagaa tgactggata aatggtggca
     5161 atccatatct tgattgacgg ccagccagat ttactgcatt atcggtgaat ccacatccgg
     5221 tgccggccac attgggagaa ttgatccgtg acaggtatgc gctgccattc cgggcaatat
     5281 acatacgatt atcaggcccc agttgaaggg caccgatcgg gtcggcgtcc tgggcgacac
     5341 taccgaccat cacttttgaa tccaggattt catccggatc attggtcgat acgtcatatt
     5401 gccagatgcg cttgttggcc ttccattccc caatgtaaag gaagtggctg ttaggtgaga
     5461 attcaacgcc ataaggtcca ccgggatcag aacctcccgg gttgacataa ttataatcgg
     5521 ttacaagatg agaaactgtt ccgctggagt tgctgaaatt actgatcgaa acatggaaag
     5581 cggtattatt ggcaatacca attttcgaac catccggcga gactttaata taccctttgg
     5641 aggcttccat atccccggta atggctggtc cggtatggct tatcaccggt gtgacattga
     5701 ccccgttggt tgttacttca aatgcgtaat atgcatctga attccagaga tgtgtaatta
     5761 cccagataga tataccgttg ctatgcccta ccgcagtcac tttttcacaa gcatacggaa
     5821 gaagggagat gtttttttca gtggtcacca catcacccag gccaccttca agggtcatat
     5881 ctacacgtga ataacacagg ccggcagcaa gaccgttatc cttggcatcg acggtgaaaa
     5941 tatagtactg ggttgtactt cccggtttgg ggacaatgat ccccgactgg gtgctggagg
     6001 aatgccccag caatccgctt ccgtgcggca tctggtcgtg ttcgcggtta tatacgaatc
     6061 ggccgtcggt ataaaactcc aggttgcctg aggatgtgga tatagacgag cagccttccc
     6121 cggtgttgag agccccgttt gtcagtggta ccggtgctcc catggcgaag ctcaggcccg
     6181 cattaacgcc gaaataccag taatttgcct gcttttgtcc catagagctc tgacacacta
     6241 aaaggacagt ggagatgatc agcaatcttt ttaaagccac tttgaatgaa gattaaagat
     6301 gataaatgta aaaataatat taattcatga ttttcaatca taatgattta tgtttttatt
     6361 cgtggctttt acccaaacat attttttaat gcaaccagta gaaaatttac tttgtaaacg
     6421 ataaatcaat ttgtttaaaa atatctatct ttatcctgaa aaatgccggg ccaattttga
     6481 agaatatgtt taaacccaat atcataaaat gaactgacaa aagcaaatta actactaaac
     6541 tattgattat gagaaaactg ttactattta tttcggctct gtttctcatt catatgagtt
     6601 acgggcaatt gacgggaatc aaatcgatcc ccggcgatta tgcgacgatt gaagcggcca
     6661 tcactgcact taacgcgcag ggtgtaggag ccggaggagt gacctttaat gttgctgccg
     6721 gccatacaga aacctttacc accgtgaccg ccggtttggt taccgccagc ggaacagcgg
     6781 gcaaccagat catttttcag aaaagcgggg caggcgctaa tccgctgatt acagccgcca
     6841 ctcccggaac cggaacgatg gattacatca tctgtctttc aggtgctgat tacgttacgt
     6901 tcgatggtat caacgtccag gaaaatgccg ccaataccac ctcagccatg cagatggaat
     6961 gggcgtatgc agtattaaaa gcgtctgtgg ataacggttc acaaaataat acgataaaga
     7021 attgtgcgat aagcctgaat tcagacaata ctgcaagtta tgcagtctat tcaaacaatc
     7081 acac
//
LOCUS       DAOPNH010000181         7049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12138_length_7049_cov_7.600801, whole genome shotgun sequence.
ACCESSION   DAOPNH010000181 DAOPNH010000000
VERSION     DAOPNH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_7049_cov_7.600801"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKZ74_09880"
     CDS             <1..679
                     /locus_tag="PKZ74_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93107.1"
                     /translation="GTDIVERGGAQSLLIMNDSTVYIGLTWTDNPMYDPAYCDIIKTD
                     TMGEIILQRRLIDDAYPPTSIIKSFDDKILAIGYYHVDSNWDIYLWKMNQDLEDDTLY
                     TQPITYDSLCPYEIQSDTVALDCGLFVNIDELPTQEQYESSIKISPNPASDWVVLTLP
                     DVVAPGAVEVQVYDVFGREAWGQGGVEKMPVNRMVLLDVLGYPAGIYVVVVTDKKGRR
                     YTGKIVK"
     gene            complement(691..2082)
                     /locus_tag="PKZ74_09885"
     CDS             complement(691..2082)
                     /locus_tag="PKZ74_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HPM93108.1"
                     /translation="MELQKKFLKDAEKVAFDPEHRRKLNHNISKYQVNVEKGKKQFND
                     LELARKKAANTRHKILNDLDKYLIEFESNFEKNGGKVIWASTAKDAVKEIMAIMKNTK
                     AVSVVKSKSMMTEELELNEILEKKKIEAIETDLGEFIVQLAGEKPYHILTPAMHKSKE
                     DVAKLYHEKFGMPLDSTPAAIAAYTRNYLREKFLSAGVGITGANFLLSDLGAVCLTEN
                     EGNGLMSISFPKVHIVIAGIEKILPSISDLQLFWPLLATMGTGQKVTAYNSIVFGPRH
                     EDEADGPAEMYVVLLDNRRTEVLKQEKVRRALSCIRCGACLNACPVYKNIGGYTYSSV
                     YTGPIGSVITPWMEDMGEYKHLSFASSLCGNCTDVCPVHIPLHELLLVNRQKSVEMGY
                     AKSGEKFMMKNMKRVLLSRKLMNMRGGFKNMLFRNFFASAWGPRRELPRLEAKTFNKL
                     WREKQERLMKEES"
     gene            complement(2114..2563)
                     /gene="rpiB"
                     /locus_tag="PKZ74_09890"
     CDS             complement(2114..2563)
                     /gene="rpiB"
                     /locus_tag="PKZ74_09890"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPM93109.1"
                     /translation="MKEKTSIIPIACDHGGYELKEFIKMNLLKDGYEIKDFGTDGPES
                     VDYPDFIHPLAKGVNDGVYPLGIIICGSGNGAQMVANKYPNVRAALCWNEEIARLARL
                     HNNANILSLPGRFVSKEEGWNMVKVFLETSFEGGRHQKRVEKINFKF"
     gene            3159..4124
                     /gene="nadA"
                     /locus_tag="PKZ74_09895"
     CDS             3159..4124
                     /gene="nadA"
                     /locus_tag="PKZ74_09895"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPM93110.1"
                     /translation="MAEPVSQNTKIDFKKEFIRLRKEKNAILLAHYYQVDEIQDIADY
                     VGDSLGLAQQAAKSDADIIVFAGVHFMAETAKILNPETKVLIPDMEAGCSLADNCPYE
                     SFTEFIKQHPGHIVISYINCSAAVKSVSDIICTSGNAVQIVESFPKDQKIIFAPDKNL
                     GGYINRITGRNMVLWDGTCEVHDILQTEAIIKLKIQNPDAKLIAHPECKAPILEIADF
                     VGSTTALLNFTINDDARKYIVATETGILFQMRKSSPEKEFIIVPSDETCSCNDCPYMK
                     LNTMQKLYLCLKNERPEIVMDPVLMEKARLPIERMLEISKKARLI"
     gene            4121..5737
                     /locus_tag="PKZ74_09900"
     CDS             4121..5737
                     /locus_tag="PKZ74_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPM93111.1"
                     /translation="MKWFKAFRRLKPSVEKMEAFRRLKPSVPGILLLIFIGLSYCGYA
                     QEEAKTLSDGYQVFYYPNGNKSSEGTMRNGKPDGYWKTYNEAGKLVSEGNRKDFELDS
                     AWKFYDENGKKALEINYLKGKKEGIRKTWREDEIIEEMFKNDIKNGLTRYLYPDGKLK
                     KEVNFREGLEEGMSKEFDTDGRVITIITYKSGFITGREIINRYDNTGRKHGPWKYFYD
                     DGTLRMEGSYKHGMENGYFKEYDRNGNLLTTSKFADGVKQENVAELVKLEVRKDYYPD
                     GKIKVAATYNKEGKPEGIRREYAEDGTVEKSYIFRNGIMIGEGVVTEKGERDGFWKEY
                     YDDGKLRAQGKYNKDLKEGPWVFYHPNGQTEQEGSYFKGKPEGEWRWYYAGGQLLREE
                     TCFNGLADGDMKEYDEAGNIITRGKYIEGREDGDWYYKVGDNETEGSYTDGMRNGMWR
                     YRDLSPDGKQGTLRFEGRYIEDNPHGKHTYYWENGMKKEEGEYVNGRKEGDWIIYNPD
                     GTPFIVVSYSDGAEVRFDGIQVSGTRPDEN"
     gene            5783..6232
                     /locus_tag="PKZ74_09905"
     CDS             5783..6232
                     /locus_tag="PKZ74_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPM93112.1"
                     /translation="MKKFTFLAVLAVLFFTTDAIFAQDVKAKADEGSAVVITPEKFQD
                     YASENIGGLVEIKGMVIHVCRHGGKKMFIIGEDPEMRVKINASDKVSVFEPEVEGSVV
                     VVKGVIEPIEEEVVPDAEKATEDTDHKNYYHKPQYAISCNSFRIVEE"
     gene            6234..6800
                     /locus_tag="PKZ74_09910"
     CDS             6234..6800
                     /locus_tag="PKZ74_09910"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPM93113.1"
                     /translation="MMQFQWRKWFRVVHRDFGYLFFGLTLVYSISGIALNHISEWNSN
                     YIIVRKEITLDNPSRIQKGISKDEVRSLMDEIGERRSYKNHYFPQSDQLKVFLKGGSV
                     VINTETGNGLLEKVTRRPFFREMNYLHYNPQESWTWISDVFAGALIILAITGLFLVRG
                     AKGITGRGAWLTVLGIIIPIIFLIIYFY"
     gene            6834..7010
                     /locus_tag="PKZ74_09915"
     CDS             6834..7010
                     /locus_tag="PKZ74_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93114.1"
                     /translation="MTAIWFSILFTAWYILALVVAEKVGKKRRIGEEWSFFLSFMFSP
                     LVGLVVSLMTKGKD"
BASE COUNT         1909 a         1581 c         1850 g         1709 t
ORIGIN      
        1 tggcacggat attgtggaac gaggtggggc tcaatcgttg ttgatcatga atgattcgac
       61 tgtctatatt ggtttaacat ggacagacaa cccaatgtac gatcccgcat attgtgatat
      121 aattaaaact gacacaatgg gagagattat tcttcaaaga agattgatag atgatgccta
      181 cccaccaaca tcaattatta aatccttcga tgataaaatt ctagcgattg gctattacca
      241 tgtcgatagt aactgggaca tctatctgtg gaaaatgaat caagatctag aggatgacac
      301 tctttacacc cagccaatta cttacgacag cctgtgcccc tatgagattc agtcagatac
      361 cgtggcgctg gattgcggcc tgtttgtaaa cattgatgaa ttgccaacac aggaacaata
      421 tgaatcatcg ataaaaatat cgccgaaccc ggcaagcgat tgggtggtgc tgactttgcc
      481 ggatgtcgtg gcgcccgggg cggtggaagt gcaggtgtat gatgtgtttg gaagagaggc
      541 atgggggcag ggtggcgtgg aaaaaatgcc ggtgaaccgg atggtgctgc tggatgtttt
      601 gggttatccg gcgggaatat atgtggttgt ggtgacggat aaaaaaggca ggagatatac
      661 agggaagata gtgaaataat tacgctattt ttagctctct tccttcatca gccgctcctg
      721 tttctcgcgc cagagcttgt taaaggtctt agcttcgagc cggggcagtt cgcgacgcgg
      781 gccccaggcg gatgcgaaga aattgcggaa cagcatgttc ttgaacccgc cgcgcatgtt
      841 catcagcttg cggctcagca aaacacgctt catgttcttc atcatgaatt tctcaccgct
      901 tttggcatag cccatctcca cactcttctg ccggtttacc agcagcagct catgcaacgg
      961 gatgtgcacc gggcagacgt cggtgcagtt gccgcacaac gacgaggcaa agctcaggtg
     1021 cttgtattcg cccatatctt ccatccaggg cgtgatgacc gagccgatcg ggccggtgta
     1081 gaccgacgaa taggtatagc cgcctatgtt tttatagaca ggacaagcgt tgaggcaggc
     1141 gccgcagcgg atgcacgaca acgctctccg taccttctcc tgcttcagca cctcggtgcg
     1201 ccggttatcc agcaaaacca cgtacatctc cgcaggcccg tcggcctcgt cttcgtgccg
     1261 cggtccaaac acaatgctgt tgtaggctgt gaccttctgc cctgtcccca tggtggccag
     1321 cagcggccag aagagctgca ggtcgctgat ggaaggaagg atcttctcaa tgccggcaat
     1381 gacgatgtgc accttcggaa acgaaatcga catcaggccg ttgccctcgt tctcggtgag
     1441 gcagacggcg cccaggtccg acaacagaaa gttggccccc gtaatgccta cgccggcact
     1501 tagaaacttc tccctcaggt aattccgggt gtaggccgcg atggctgccg gcgttgaatc
     1561 aagcggcata ccgaactttt cgtggtacag ctttgccaca tcttccttcg atttgtgcat
     1621 cgccggggtc aggatgtgat atggcttttc gccggccagt tgcacgatga attcgcccag
     1681 gtcggtctca atggcttcga tcttcttctt ttccagtatc tcgttcaact ccagctcctc
     1741 ggtcatcatc gacttcgatt tgaccaccga aacagccttt gtattcttca tgatggccat
     1801 gatctcttta acggcgtctt ttgccgtaga ggcccagatc acctttcccc cgttcttttc
     1861 aaaattgctc tcgaattcga tcaggtactt gtcgagatcg ttgaggatct tgtgccgggt
     1921 gttggcggct ttcttccgcg ccagctccag atcattgaac tgtttcttcc ctttttctac
     1981 attgacctgg tatttcgaga tgttatgatt gagcttccgc cggtgctccg gatcgaaagc
     2041 aactttttcc gcgtctttca gaaacttttt ctgcaattcc atgacaacaa cagttcaatt
     2101 aaaaattcaa aattcagaat ttaaaattaa ttttttccac tctcttctga tgtctgccgc
     2161 cttcgaaaga tgtttccagg aaaaccttca ccatattcca tccttcttct ttagaaacga
     2221 accgtccggg aagggaaagg atattggcgt tgttgtgaag ccgcgccagg cgggcgatct
     2281 cttcgttcca gcacagcgcc gccctgacgt tcgggtattt gttggccacc atctgggcgc
     2341 cgttcccgct cccgcagatg atgataccaa ggggataaac gccatcattg acccctttcg
     2401 ccagcggatg gataaaatcg ggataatcga cactctccgg accatcggtg ccaaaatctt
     2461 ttatctcata tccatctttc aataagttca tctttatgaa ctctttcagt tcatatccgc
     2521 catgatcgca ggcaatcggg atgatactcg ttttttcttt cactattaac tgattgtttt
     2581 aagcaaagtt aacctttatt caccataaaa attctcttca ggttgtatta aaagtctttt
     2641 atttaaccac aaaggcgcac aaaggaatac actaaggaac acaaaggttt tatcctgcta
     2701 atcaatgact tggtgtgcct ttgtgaaggc ccttcgtgcg cctttgcggt taaacatttt
     2761 taagacataa acagaaacta acattgacct gttaaaagct gttaataacc ctgtataaat
     2821 ggcaccggtt ttgttaacaa ataaaaaatt cgcgttaaaa tcgattttat gcggttttta
     2881 tgatgagata tgaagaaaat tcaccctctt tataaacatc ctcaacctgt tcataaccct
     2941 gaatttttta acaccccggg ttatcgacag gttgttaata attcttaatt ttccgaaaat
     3001 caaataataa taaagaaatt tgcagaaaat acctgttaac aaaattgaat aactcggttt
     3061 gtcaaatttt tgaaggataa gttttcatca ttatcaacag gcataataaa aagaataaat
     3121 ttctttaaac tttaatatta ataaggaatt gataaaagat ggcggaacca gtcagtcaga
     3181 atacaaagat cgattttaaa aaggaattta tccggttacg gaaagaaaag aacgccatcc
     3241 tcctggccca ctattaccag gtagatgaaa tccaggatat tgccgattat gtgggcgaca
     3301 gcctgggcct ggcacaacag gccgccaaat cggatgcgga tatcattgtt tttgccgggg
     3361 tgcactttat ggcggaaacg gctaaaattc tcaacccgga gacaaaagtt ttgattcctg
     3421 atatggaagc aggctgttcg ctggccgaca attgtcctta cgaatcattt accgaattca
     3481 tcaaacaaca ccccggacat attgtaattt cctatataaa ttgcagcgct gcggtaaaat
     3541 cggtatcgga tatcatctgt acttcgggaa atgccgttca gatcgtcgaa tcatttccga
     3601 aggaccagaa gatcattttt gctccggata agaacctggg cggttatatc aaccgcatta
     3661 cgggaagaaa catggtttta tgggacggta cctgcgaagt ccatgacatc ctgcagacag
     3721 aagccatcat caaactaaaa atacagaacc ccgacgccaa actgatcgcc catcccgaat
     3781 gcaaggcccc gattcttgaa attgccgatt ttgttggatc aacaacagca ttgctgaatt
     3841 ttacgatcaa cgatgatgcg aggaaatata ttgtcgccac ggaaacggga attcttttcc
     3901 agatgaggaa atcgtcgccg gaaaaggaat tcatcatcgt tccgtcggac gaaacctgct
     3961 cgtgcaacga ttgtccttac atgaagctga acacgatgca gaaactctac ctgtgcctga
     4021 aaaatgaacg tccggagatc gtaatggatc cggttttgat ggaaaaggcg cggttaccga
     4081 tagaacggat gctcgaaatt tcgaaaaaag ccagattgat atgaagtggt ttaaggcatt
     4141 ccgacgacta aagccgtcgg ttgaaaagat ggaggcattc cgacgactaa agccgtcggt
     4201 tcccggaatt ttattattga ttttcattgg tttaagctat tgtggatatg cgcaggaaga
     4261 ggcaaagacc ctttctgacg gctaccaggt tttctattat ccgaacggga ataaatcgag
     4321 cgaagggacg atgcgcaacg ggaagccgga tggttactgg aaaacgtata atgaagcggg
     4381 taagctggtt tcggaaggga acaggaaaga ttttgagctc gacagcgcct ggaaattcta
     4441 tgatgaaaat ggaaaaaaag ccctggaaat caattatctg aagggaaaaa aggagggtat
     4501 ccggaagacc tggcgggaag acgagatcat cgaagagatg ttcaaaaatg atataaagaa
     4561 cggcctgacg agatatttat atcctgacgg gaagctgaaa aaggaggtaa atttcaggga
     4621 agggctggaa gaggggatgt cgaaggagtt tgataccgat ggcagggtca tcacgatcat
     4681 cacgtacaaa agcggtttca tcaccggccg ggagatcatc aaccgttacg acaacacagg
     4741 caggaaacac ggcccgtgga aatactttta cgacgacggg acgttgcgga tggaggggag
     4801 ctacaagcac ggcatggaga acggctattt caaggagtat gaccgcaacg gcaacctgtt
     4861 gaccacgtcg aaatttgccg acggggtgaa gcaggagaat gtggcggagc tggtgaaact
     4921 ggaggtccgg aaagattact accccgacgg taagatcaag gttgccgcaa cgtacaacaa
     4981 agagggcaaa ccggagggga tccggcggga atatgccgaa gacggcacgg tggagaagtc
     5041 gtacattttc cgcaacggca tcatgatcgg cgagggtgtt gtgacggaga agggggagcg
     5101 cgacggtttc tggaaggaat attacgacga cggcaagctg agggcgcagg gaaaatacaa
     5161 caaagacctg aaagagggtc cgtgggtgtt ttaccatccg aacggacaga cagagcagga
     5221 gggaagctat ttcaaaggca aaccggaagg ggagtggcgg tggtactacg ccggcgggca
     5281 gcttctgcgg gaagagacct gtttcaacgg gctggcggat ggcgatatga aggagtacga
     5341 cgaagcggga aacatcatca cccggggaaa gtatattgaa ggccgggaag acggcgactg
     5401 gtactataaa gtcggcgaca acgagacgga aggaagctat accgacggga tgcgcaacgg
     5461 catgtggcgc taccgcgacc tgtcgcccga cggcaagcag ggaaccttgc gttttgaggg
     5521 ccgttacatt gaggataatc ctcacggaaa gcatacctat tactgggaga acgggatgaa
     5581 gaaggaagaa ggggagtatg tgaacggccg gaaggagggc gattggatca tctacaatcc
     5641 cgacgggacg ccgttcatcg tcgtgtcgta cagcgatggc gccgaggtgc ggttcgacgg
     5701 gatccaggtg tcaggcaccc gcccggatga gaattaaaac ttttgttaaa tttgcacgca
     5761 aaaccaataa aagaaagaac atatgaaaaa attcacattt ttagctgtat tagccgtatt
     5821 gttttttaca acagatgcga tttttgccca ggatgtcaaa gcgaaggctg acgagggcag
     5881 cgctgtagtc ataaccccgg agaagttcca ggattatgct tctgaaaaca tcggcggcct
     5941 ggtcgagatc aaagggatgg tcatccatgt atgccggcat ggcggaaaga agatgttcat
     6001 catcggggaa gacccggaga tgagggtaaa gatcaatgcc agcgataagg tcagcgtatt
     6061 cgagccggag gtggaaggca gcgttgtcgt ggtgaagggc gttatcgaac cgatcgaaga
     6121 agaggttgtt ccggatgcgg aaaaggcgac cgaggatacc gatcataaaa attactacca
     6181 caagccccag tatgcgattt cgtgcaactc gttccgcata gtggaggaat aagatgatgc
     6241 agtttcagtg gaggaaatgg ttcagggttg tccaccgtga tttcgggtat ttgtttttcg
     6301 gactgaccct tgtttattct atttctggga ttgccctgaa ccacatttca gaatggaatt
     6361 ccaattacat cattgtcagg aaggagatca cgctcgataa cccgtcgcgg atacagaaag
     6421 ggatctcgaa ggacgaggtt cgcagcctga tggatgagat cggggagcgg cggagctaca
     6481 agaatcatta tttcccgcag agcgaccagc tcaaggtctt tctcaaaggc gggtcggtgg
     6541 taatcaacac cgaaaccggc aacgggctgc tggagaaagt cacgcgccgg ccttttttcc
     6601 gggagatgaa ttacctgcat tacaatccgc aggagagctg gacgtggatc tccgacgttt
     6661 ttgccggcgc gctgatcatc ctggcgatca cggggctgtt cctggtgcgg ggagccaagg
     6721 gcattacggg gcgcggtgcc tggctgacgg tgctcgggat cattattccc ataatattcc
     6781 tgataatcta tttttattaa gaacccgaaa cctggaacct gaaaccaaaa actatgaccg
     6841 caatctggtt cagcatcctg ttcaccgcct ggtacatcct ggccctggtc gtagccgaaa
     6901 aagtcggcaa gaagaggcgc atcggggagg agtggtcgtt tttcctgagc ttcatgtttt
     6961 cgccgctggt ggggctggtt gtcagcctga tgacgaaggg gaaagactga ggggtcactg
     7021 gtcataagct cgctaacgct cgcgtcatt
//
LOCUS       DAOPNH010000182         6988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12290_length_6988_cov_5.414539, whole genome shotgun sequence.
ACCESSION   DAOPNH010000182 DAOPNH010000000
VERSION     DAOPNH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_6988_cov_5.414539"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKZ74_09920"
     CDS             <1..549
                     /locus_tag="PKZ74_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93115.1"
                     /translation="RDNGIPKKWVSYIKNCISQIAPHYTMKRMLDDYQHKYYDRLIRR
                     SKDIRSDDYQLARDIEAWKSKMRAGWNHIEVRRIAVPDPARKSLDLGDNFIAEIELKL
                     NGIKGEDLGLDILFGQKEMDTVNKIMHKEEMKMVDSAPGVAKFTCKIPMEKIGVYHYA
                     FRLYPKKEWLAHKQDFNLVKWL"
     gene            599..1342
                     /gene="fabG"
                     /locus_tag="PKZ74_09925"
     CDS             599..1342
                     /gene="fabG"
                     /locus_tag="PKZ74_09925"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPM93116.1"
                     /translation="MLLKDKTALITGAARGIGRAIALQFAAQGADVAFTDLRADENME
                     SLEKEIAAMGRKGKGYTSDASDFNGAEAVVDQIVNDFGKIDVLVNNAGITRDNLLMRM
                     TEADWDLVIKVNLKSVFAMTRAVQKYMLKQRFGSIINMSSVVGVSGNAGQSNYSASKA
                     GLIGFTKSIAQELGSRNIRCNAIAPGFIETEMTAKLPQDVREAWIKDIPLKRGGKPED
                     VAHIALFLASDLSDYVTGQVINVCGGMNT"
     gene            1347..3452
                     /locus_tag="PKZ74_09930"
     CDS             1347..3452
                     /locus_tag="PKZ74_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM93117.1"
                     /translation="MPELFTGQPSWLIIVCLALAFGYATSLYFRDRKNEFPGYLKTIL
                     GIVRFLAIFFISFLLLAPFVKTTSKEKEKPLLIFALDNSESVIIHKDSLAVKSELPAI
                     LDNLADKLTERGEAQKYYFDEKVYRLDGSADYNSSAAFNGKATDIANAVGEIVNIYTN
                     RNIGALILVSDGIFNTGTNPLYRAKDIPFPVYTVALGDTSIRKDLVVAGVNYNRMVYL
                     NNKFPLELVVRAGNAAGNQSRLRIVQQDNVLFSQDFQVNDDDFTQTFRIVLEAKRKGL
                     QKFTVIIDPIDGELSYSNNRKDIFVEVLDAKSRVLILSGAPHPDISALRQAILSNMNY
                     EVDVSTLNDFTGKLDSYSLVILHQLPSVNFPAENITREITEKAIPVLFILGKQSDLAR
                     VNQSKPGLSIVTARPSFEEAVPKYNPAFSAFSLSESTVSWLSDLPPLVAPLGDYQVAN
                     AARVLVSQRIGSVETTRPLILLNETLEGRKGIIAGEGLWKWRLFNFARFQNHQSFNEL
                     INKLVQYLSLKDQKKNFRVYQPGSFRETTPVIFDAEVYNDNYELTTLPEVQLSIINEE
                     GKTFPYVFNKTGNSYHLDAGAFPPGNYSFRAETSLDGRTLYETGEFSVLSLDLEALNT
                     VADHNLLYQISSETGGEMFYPADLDKLASAILEREDIRPLTYTGKKYEDLLNKSWILL
                     LILGLLTLEWFVRKRAGSY"
     gene            3466..4695
                     /locus_tag="PKZ74_09935"
     CDS             3466..4695
                     /locus_tag="PKZ74_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPM93118.1"
                     /translation="MEKILFVLIIAIVVFEFLLERVLDYLNATCWSEKLPEELQGIYE
                     QEKYSRSQQYLKANHWFSQLTESFNFILVMLMLLLGGFAFLDNQVRAITDNPVLMALL
                     FFGAIGVVADFLGTPFSIYSTFVIEQRFGFNKTTVRTFILDKIKGWVLAAVVGGGLLA
                     LIVWIYGVTGSWFWIIAWGVISLFTILMTLFYSNIIVPLFNKQTPLEPGELRDAIEEF
                     AGKTGFSLKNIYVIDGSKRSTKANAYFTGLGRKKRIVLYDTLIKDHPVDELVAVLAHE
                     IGHYRKKHTLIGTLISVLQTGLILFILSRFLDNPHLSGALGASQPSFHLGLVAFGILF
                     TPISLITGLGLNILSRMHEFQADRFAAVNHNAESLRNALVRLSVNNLSNLRPHDLYVF
                     FHYSHPPLLKRLKRLSE"
     gene            4786..5664
                     /locus_tag="PKZ74_09940"
     CDS             4786..5664
                     /locus_tag="PKZ74_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93119.1"
                     /translation="MKKFHTLGKAVISVVCICLMHNFSVAQVADVYNGTVLPQVKETI
                     QAPVMEPVRSLELYNNGPLITHYGIGYNGADISELQNPSLGMTVSGFGHQFSLGYKIA
                     DDFTVTDPGGWNIVGIAFYAYQAGSPSTSNFTAVYFAIYDGDPMLPGSNIIYGDFVTN
                     RMTSTGFTGIYRVLETAPTDNNRPIMVNHCEFDLTLGPGTYWLVWMSDGADIYGYEWA
                     PPVTILGQAPTGNGLQFTTAWGGAYDSGTMTQQGFPFVIFGRAGQAAIPVAGWALGIG
                     LVLIIGLAVLRYRRIL"
     gene            complement(5667..>6988)
                     /locus_tag="PKZ74_09945"
     CDS             complement(5667..>6988)
                     /locus_tag="PKZ74_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93120.1"
                     /translation="NTEPAQDHLVENEYNDLNGEELLAYLRDSVVGKDITVEAGGENV
                     TYLNLDHGASTRAFWPVWEAYRHALNAGPDRRSEITGNTRRIISGFLGAPAEEYDIVF
                     TSNTTEALNLAAESLSLEAGEETEPVVINTVLEHNSNELPWRFAKGIHHERLNVDAQG
                     FIDLDELDTKLSEYNEQGRHGKRRVAIVAVSGASNVLGTCNDLEAIGRITHKYGARLL
                     VDAAQLMAHRPVDMAGTGIDYLAFSGHKIYAPFGSGALIVRKGLLKFNDQETSQIHDS
                     GEENLAGIAALGKSLELLERVGMDIVMKEEINLTAQFLEGMNRVPGIRIYGIKSSLDS
                     NFTRKAGVISFDFKKIIAHTVAKRLANECGIGIRSGCHCAHLIVKRILGVAPPFEGIQ
                     RLIVLAAPGLNLPGVARVSLGIENTGEDAERLVKSLETIVGKKRRLN"
BASE COUNT         1767 a         1744 c         1679 g         1798 t
ORIGIN      
        1 agggataacg ggattcccaa aaagtgggtg tcttatatca agaactgtat ttcccagatc
       61 gccccgcatt atacgatgaa aaggatgctc gatgactatc agcacaaata ttatgacaga
      121 ctgatcaggc gttcgaaaga tatccggagt gatgactatc agcttgcccg cgatatcgaa
      181 gcgtggaaat caaaaatgag ggccggatgg aaccatatcg aagtcaggcg gatcgccgta
      241 ccggacccgg cccggaaatc cctcgacctg ggcgataatt ttatcgcgga gatagaactg
      301 aaactcaacg ggatcaaagg tgaagacctt ggtttagata tccttttcgg acaaaaggag
      361 atggataccg ttaataaaat tatgcataaa gaagagatga agatggtgga ttcggctccc
      421 ggcgttgcaa aatttacatg taaaatcccg atggagaaaa taggcgtgta ccattatgcg
      481 ttcaggttat atccaaaaaa ggaatggctc gcacataaac aggattttaa cctggtcaag
      541 tggctgtagc ccgatgattt ttagcttttt tattcgtaaa tttgaaaaaa attgacctat
      601 gctgctgaaa gataagaccg ctctcataac aggcgctgcc cgtgggattg gacgggccat
      661 cgcgcttcaa tttgccgctc agggcgctga tgttgctttt accgatctgc gggccgatga
      721 aaatatggaa agccttgaaa aagagattgc cgcaatgggc aggaaaggca aagggtatac
      781 ttccgatgcc agtgatttca atggagctga agctgttgtc gaccagatcg tgaacgactt
      841 cggtaaaatt gatgttctcg tcaataatgc gggaattacc cgggataacc tgctgatgcg
      901 aatgaccgaa gccgactggg acctggtgat caaagtcaac ctgaaatcgg tcttcgccat
      961 gacccgggcc gtgcagaaat acatgctcaa acaacgcttc ggatccatta tcaatatgag
     1021 ctccgtcgtc ggtgtcagcg gtaatgcagg gcaatccaat tattctgctt ccaaagcggg
     1081 cctgatcgga tttaccaaat ccatcgcaca ggaactcggc tcccgtaata tccggtgtaa
     1141 tgccattgcc cccggattta tcgaaacgga aatgacggca aagctgccgc aggatgtcag
     1201 ggaggcatgg attaaagata ttccgttgaa aaggggaggc aaacccgagg atgtagccca
     1261 catagccctg ttccttgcct ccgacctgtc ggattatgtc accggccagg tgatcaacgt
     1321 ctgcggagga atgaatacct gatctgatgc ccgaactctt taccggacaa ccttcctggc
     1381 tgatcattgt ctgcctggcg ctggcattcg gctatgccac gagcctctat ttccgcgacc
     1441 ggaaaaatga gttccccggt tatcttaaaa ccatcctggg cattgtccgc tttttggcca
     1501 tcttttttat ttcattcctg ttattggcac cgtttgtcaa gaccacttca aaggagaagg
     1561 aaaaaccttt gctgattttc gccctggata actcagaatc ggtgatcatc cacaaagatt
     1621 cccttgcggt taaaagtgaa ttaccggcta ttctggataa cctggccgat aaactcaccg
     1681 aaaggggcga agcccagaaa tactattttg atgagaaagt gtaccggctg gatggcagtg
     1741 cagattacaa cagttccgca gcctttaatg gcaaagcgac ggatatagcc aatgcagtcg
     1801 gtgaaatcgt taatatatac accaatagaa acataggagc gctgatcctt gtatccgacg
     1861 ggatattcaa taccgggacc aatccactat accgggcgaa agatatacct tttccggttt
     1921 atacagtcgc cctgggcgat accagcatcc ggaaagacct ggtggttgca ggcgtgaatt
     1981 ataaccggat ggtttacctg aataataagt tcccgctgga actagttgtc agagccggca
     2041 atgccgccgg taaccaaagc aggctgagga tcgtccagca ggataatgtc ctgttctccc
     2101 aggactttca ggttaatgat gatgatttta ctcagacatt caggatcgtg ctggaagcta
     2161 aaagaaaagg acttcagaaa tttaccgtca tcatcgatcc gatcgacggg gaattgtcgt
     2221 attccaataa ccgaaaagat atttttgtgg aggtgctcga tgcaaagagc cgcgtactca
     2281 tcctttcagg agcaccgcat cccgatataa gcgcgctgcg ccaggcgata ttatcaaata
     2341 tgaattatga ggttgatgtt tccacgctga atgattttac aggaaagctg gatagttaca
     2401 gcctcgtaat cctgcatcaa ctgccttcag tgaatttccc tgccgaaaat atcacccggg
     2461 aaataaccga aaaggccatc ccggtcctgt ttatacttgg aaaacagtct gacctggccc
     2521 gggttaacca gtcgaagccg gggttaagca tcgtaaccgc ccggccgtcg ttcgaagagg
     2581 ccgtaccgaa gtataatccg gccttttccg ctttttcgct gagtgagagc accgtttcct
     2641 ggctttccga cctgccgccc ctggtggccc ccctgggcga ttaccaggtg gccaacgccg
     2701 cccgggtgct tgtcagccag cgtatcggaa gcgtcgaaac cacccgcccg ctcatcctcc
     2761 tcaacgaaac cctcgaaggg cgtaaaggca tcattgccgg cgaaggactc tggaaatgga
     2821 gactctttaa ttttgcccgt tttcaaaacc atcaatcgtt taacgaattg ataaataagc
     2881 tggtacaata ccttagcctg aaagaccaga agaaaaactt cagggtctac caaccgggca
     2941 gttttcgcga gacaaccccc gttatattcg atgcagaagt ctataatgat aactatgaat
     3001 taactacact tccggaggtt cagttaagta taataaatga agaaggtaaa acatttccct
     3061 atgtattcaa taaaaccggc aattcctacc acctggatgc cggcgcgttc ccgccgggca
     3121 attattcctt ccgtgccgaa accagcctcg acggacgaac actttatgag accggtgaat
     3181 tttctgtgct ctctcttgac ctcgaagccc tgaataccgt ggccgaccat aacctgctgt
     3241 accagatttc cagtgaaaca ggaggggaaa tgttctatcc ggctgatctg gataaactgg
     3301 catccgcaat acttgagagg gaggatatcc gcccgctgac atacaccggc aagaaatatg
     3361 aagacctgct gaataaaagc tggatactcc tcctgatcct gggattgctg acattagagt
     3421 ggttcgtcag aaaacgggcc ggaagttatt gatcctatat tatttatgga aaagatcctg
     3481 tttgtcctca tcattgctat tgttgtcttt gagttccttc tcgaaagagt gctggattac
     3541 ctgaacgcaa cgtgctggag cgaaaaactg ccggaggaac tgcagggtat ttatgaacag
     3601 gagaaatatt cgcggtccca acagtacctg aaggcaaacc attggttctc acagcttact
     3661 gaatccttca attttatcct ggtcatgctg atgcttctat tgggaggatt tgcttttctc
     3721 gacaaccagg tcagggcgat aacagataac ccggtgttga tggcgctttt gttttttggt
     3781 gcgatagggg tggtggctga tttcctggga accccttttt ccatctattc aacctttgta
     3841 atcgaacaaa gattcggatt caataaaacc acagtcagga cttttatcct ggacaagatt
     3901 aaaggatggg tgctggcagc ggtggtaggc ggggggctgc tggctttgat cgtctggata
     3961 tacggcgtca cgggaagttg gttctggatc atcgcctggg gcgtcatcag cctctttacg
     4021 attctgatga ctttatttta ttccaacatc attgtgcccc tgttcaacaa acaaacaccg
     4081 cttgagccgg gtgaactgcg cgacgctatc gaggaatttg ccggtaaaac cgggttcagc
     4141 ctgaaaaata tctacgtgat cgacggctcg aagcgatcaa ccaaagccaa tgcctatttt
     4201 acgggattgg ggcgcaaaaa gcggatcgtt ctttacgata ccctgatcaa agatcatccg
     4261 gtcgacgaac tggtggctgt cctggctcat gagatcggcc attaccggaa aaagcatacc
     4321 ctgatcggaa cgcttatttc tgttttgcaa accggcctga tcctttttat cctcagccgc
     4381 ttcctcgata atccgcacct ttcgggcgcc cttggtgcca gccagcccag cttccacctg
     4441 ggactggtcg ccttcggcat tctgttcact cccatatcgc ttattacggg gttgggattg
     4501 aatatcctgt cgcgcatgca tgaattccag gctgatcgct tcgcagccgt gaaccacaac
     4561 gcggaaagcc tgcggaatgc gctcgtccgt ttatctgtca ataatttaag taatctccgt
     4621 ccgcacgatc tttacgtttt cttccattat tcccatccac cactcctcaa acgattgaaa
     4681 aggttaagtg aatgaccgaa aaatgaacgt gaggttttat agaaatttat tgcttttctg
     4741 atcccgaatc attatattgc atcaaaattt aaaaaccaat taattatgaa gaaattccat
     4801 accctcggaa aggcggttat ttccgtcgtt tgcatttgtc tgatgcataa cttctctgta
     4861 gcacaggttg ccgatgttta taatggtacg gtattgccgc aggtaaaaga aactatccag
     4921 gcaccagtaa tggagccggt cagaagcctg gaattataca ataacgggcc gctgatcact
     4981 cattatggta tcggttataa tggggccgat atcagtgaat tacaaaatcc ttccctgggc
     5041 atgaccgttt ccgggtttgg tcatcagttt tcactgggtt ataaaattgc cgatgatttt
     5101 accgttaccg atccgggtgg atggaatatc gtgggtatcg cattttatgc ctatcaggcg
     5161 ggttcaccct ccacatcaaa cttcaccgcg gtctattttg ccatatacga tggagacccg
     5221 atgttgccgg gctcaaatat catttatggt gattttgtaa ccaacaggat gacctctacc
     5281 ggctttaccg gaatatacag ggtccttgag acagcgccta ctgacaataa ccggcccatt
     5341 atggtcaatc actgcgaatt tgacctgaca ttgggccccg gcacctactg gctggtctgg
     5401 atgtcggatg gagcagatat ctatggatat gagtgggccc ctccggttac catcctggga
     5461 caggccccta ccggcaacgg gctacaattt acaacggcct ggggtggagc ttatgatagc
     5521 ggaactatga cccagcaagg atttcctttt gttatctttg gccgtgccgg ccaggcggct
     5581 attcccgtcg ccggctgggc tctgggcatc ggtctggtcc tgatcatcgg gctggccgtt
     5641 ctcagataca gaaggatcct ttaacattag ttcaggcgcc tctttttccc gactattgtt
     5701 tccagtgatt tcaccagtcg ttcagcgtct tcgcccgtat tttcaatccc aaggctgacc
     5761 cgtgctacgc ccggaaggtt caacccgggt gcagcgagta caatcagccg ctgaatgcct
     5821 tcgaagggcg gagcgacgcc aagtatccgc ttgacgatca gatgggcaca atggcagccc
     5881 gaacggatcc ctatgccgca ctcattggcc aggcgtttgg caaccgtatg tgcgataatt
     5941 ttcttgaaat caaatgatat gacaccggct ttcctggtga aattactgtc gagactactt
     6001 tttattccat aaatccggat gccgggaacc cggttcatcc cttcaaggaa ttgcgctgtc
     6061 agatttattt cttccttcat cacaatatcc atacccaccc gttccagcaa ttcgagggat
     6121 ttacccagtg cggcaatgcc tgcaaggttc tcctcgcccg aatcatggat ttgagatgtt
     6181 tcctgatcat tgaattttag cagccctttg cgcacgatta aagcgccgct gccaaacgga
     6241 gcatagatct tatggccgga gaaagccagg tagtcgatac cggtgccggc catatcgacc
     6301 gggcggtgtg ccatcagttg cgccgcatca acaagcagac gtgcaccgta cttatgcgta
     6361 atccgaccga tcgcctccag atcgttgcac gtccccagaa cattcgaggc gccgctgacc
     6421 gctactattg ccactcttcg ttttccatga cgaccctgct cattgtattc gcttaatttc
     6481 gtatccagtt cgtcaagatc gataaaacct tgagcatcaa catttaaccg ttcgtgatgg
     6541 ataccctttg caaaacgcca tggcaactca ttggaattat gctcaaggac ggtgttgatc
     6601 accacgggct cagtctcttc gccagcttcc agtgaaaggc tttccgcagc cagattcaat
     6661 gcttccgttg tgttggacgt gaacacgata tcgtactcct ctgctggggc gccgagaaaa
     6721 ccggagatta tccgccgcgt attcccggta atttcagatc tccggtccgg gcctgcattt
     6781 aacgcatgcc ggtatgcttc ccagaccggc caaaaggccc gggtgctggc gccatgatcc
     6841 aggttcaggt aggtcacatt ttctccgcct gcttccacgg taatatcctt tccgacaacc
     6901 gaatctctca ggtatgccag caattcctct ccattaaggt cattatactc gttttccacc
     6961 aagtgatctt gtgccggctc tgtattct
//
LOCUS       DAOPNH010000183         6923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12443_length_6923_cov_6.271695, whole genome shotgun sequence.
ACCESSION   DAOPNH010000183 DAOPNH010000000
VERSION     DAOPNH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12443_length_6923_cov_6.271695"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKZ74_09950"
     CDS             <1..789
                     /locus_tag="PKZ74_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM93121.1"
                     /translation="GISEHTVLRASAGRGSRSASVIAENLSLLTTSRRFLITEKLKME
                     QAWNYGINLTRYIDILGKEMTVNAEFYRTDFTNQVIIDKEQDIHHIYVYNLDGKSFAN
                     AFQVEVKYELIRQMDVTAAFRYNDVKMTIGDELQSEPLVNKYKGLLSVSYATNLRKWQ
                     FDVTGQLNGYSRIPSTELLPVEYRRPDYSPVYTILNAQVTKFYKKWEIYLGGENLLNY
                     TQHHPIIAADDPFGPYFDASNIWGPVSGVKIYAGVRFLLKHDKL"
     gene            813..1172
                     /locus_tag="PKZ74_09955"
     CDS             813..1172
                     /locus_tag="PKZ74_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPM93122.1"
                     /translation="MKKIIALTGILVLAISISYPTMAQEKKKNYEEVQIQTSAICGMC
                     EDRIETNISFEKGVKKVQLNDDTKVVTIGYDPRKTAPDKLRTAISKLGYDADDKPADS
                     LAHAKLPVCCQKGSKPH"
     gene            complement(1219..1920)
                     /gene="tsaB"
                     /locus_tag="PKZ74_09960"
     CDS             complement(1219..1920)
                     /gene="tsaB"
                     /locus_tag="PKZ74_09960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPM93123.1"
                     /translation="MPLILNLETATQVCSVALSDDGRVVELMESGEKNSHSVTLTVFI
                     DAVMKKAGIAFSELDAVAVSKGPGSYTGLRIGVSAAKGICYGAGKPLIAISTLESMAA
                     GAKGMASLGTPSLFCPMIDARRMEVYNALYDSDLNLVREIKAEIIHEGSFLEEMKDHP
                     VLFFGDGAEKCKPLLASHPNARFLDDFQLSATYMVHLAENIFNRRAFEDTVYFEPYYL
                     KDFIPGIPRVKGLNT"
     gene            complement(1967..3310)
                     /locus_tag="PKZ74_09965"
     CDS             complement(1967..3310)
                     /locus_tag="PKZ74_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM93124.1"
                     /translation="MKFFQKKRNLFLVIGGVILIIILVILAMNKGKDRTGTKVTAEKV
                     EKRTIIEMVSANGKIQPEKDIKISPYISGEVIELYVKEGDQVKKGDLLAKIDPEIYRA
                     NYDRMMAMLNSQKAGESNAQARVAQVEAQFRNAELSFNRSKTLWEQKVISDADFDAAK
                     SSYEVAKAEKDAAFQSLKSSQYGVESAEASLKEAKENLFKTTIISPSDGTVSKLSVEI
                     GERVTGASQFSAGTEIMRIANLQSMEVNVEVNENDIVRVSLFDTCQIEVDAYLDKKFK
                     GIVTEISTSANTSGVSADQITNFNVKIRILPESYTDMIDTASAIRSPFRPGMSATVDI
                     QTETVYNILAVPIQSVTTRSDTTGTVKEEDANIDLAATKDEVKEYIFLVKDGKALLQE
                     VKTGIQDITYIEVKSGVEEGQEVITAPYRAISKRLKNGDPIKVVDKKDLYSEEEK"
     gene            3477..5816
                     /locus_tag="PKZ74_09970"
     CDS             3477..5816
                     /locus_tag="PKZ74_09970"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPM93125.1"
                     /translation="MRIISRSLLPVLCLIMLNSLSINAQIHALEATHYEISITDIDFQ
                     SGAITASTTVSLTPVSGPVDEISLELIDLAVGAVWTGNQPVSDFAQTDSLIIFPLPTA
                     LQPGDTLDVRIDYSGNPYHEGWGGFHFAGEYAFNLGVGFEIIPHNLGKSWFPCIDNFT
                     DRASYDVYCTLPAGKMAVGGGILAGISNNGNGTTTYYWKLSQQIPTYLASLAAGNYVA
                     VSDTFSGMNGQIPIDIYVRPQDANYVAGTFANLKDILSIFEECMGPYSWDRVGYTSTL
                     LGAMEHATNIFVPYGTINGSLAYESLMAHELAHMWMGDKVTCSSAEEMWINEGWATFF
                     GLYYALALNHDTNAFKQGMRANHAKVLQYCHTSSGDGSYFPLNQIPQEYTYGMSAYER
                     GSTVAQALRFYLGDSLFFEALTGFIDEFAFDDASSYDMRDFLTGFTGMDMTGFFDNYV
                     LNSGTPHYSIDSFDVSNRDVDDYDVTVYVKQKRKGPAFTGNGNVMEIMFLDGNWNRYS
                     DTIHFNGQSGCSVKSVPFLPSVVLVDPEEKMCDATTDNYKIIKNSGNYSFDKTFFSLE
                     QVTLTDSAFVQVTHNWAPPDSIKDPDSGLRISDYRYWRIDGIFPDDFQARGKFFYSVG
                     DLLDNNLILTSSDTVIILYRTGAADDWHEITFDKIGPWNIGYIFVDDIQPGEYTLAVR
                     ETGVGIGSHTWEQAETLAVFPNPSGGSFTISTVSDKGGELRIYSDSGTMVRSMTVHPG
                     KKKTKWTPGNIAAGSYYVTFHEAGRNTSESVTIIYSPGL"
     gene            5813..6922
                     /locus_tag="PKZ74_09975"
     CDS             5813..6922
                     /locus_tag="PKZ74_09975"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPM93126.1"
                     /translation="MRELSAPSGRLLSLDALRGFDMFWIIGAEGIVHALKEATGWGWA
                     VWMSGQLEHVEWNGFRFYDLIFPLFLFLSGATMPFSLTRKLEQGIPKNKIYLQVLKRG
                     LILVILGMIYNGLFRLDFENQRYASVLAQIGIAWMLAAFIFLNTSWKGQAIWVAGIVL
                     AYWAAMKLIPVPGFGPGVLTREGSLATYIDQMLLPGKMHEGIFDPEGIMVKIPATATA
                     LLGALTGWWLKNKSTGEYAKVGLMAAAAVFFFVLAYLIGLSFPINKKLWSPSFVFQTA
                     GCSVLLLALFYLLIDIWKFRKWAFFFIVIGLNPITIYMATGIINFWQISDYFLGGLAG
                     LSPEHWKNVWLITGMVAFEWLFLYFLYRKRILLRV"
BASE COUNT         1689 a         1705 c         1669 g         1860 t
ORIGIN      
        1 ggtatttcag agcatactgt tttacgtgct tccgcaggcc ggggttcacg gtcagccagt
       61 gtcattgcgg agaatctttc tttgctcacg acttcgaggc gttttcttat aacagaaaaa
      121 ctcaaaatgg agcaggcctg gaattacggt attaacctga cccggtacat cgatattctt
      181 ggaaaggaaa tgacggtcaa tgctgagttt taccgtaccg acttcaccaa ccaggtgatc
      241 atcgataaag aacaggatat ccatcacatt tacgtgtaca atcttgacgg caaatcattt
      301 gccaatgcat tccaggtcga ggtaaaatat gaattgatcc ggcagatgga tgtaacggcg
      361 gccttccggt acaacgatgt aaagatgact attggcgatg agttgcagag cgagccgctg
      421 gtcaataaat acaagggttt actatcggta tcctatgcca ccaacctgcg aaaatggcaa
      481 tttgatgtga ccgggcaact gaacggttat tcccggattc catccaccga attgctgcct
      541 gtggagtaca ggagaccgga ctattcgcct gtgtatacta ttctgaatgc ccaggtgact
      601 aaattctata aaaagtggga gatctacctg gggggagaaa acctgctgaa ttacacgcag
      661 catcatccga tcatcgctgc ggatgatcct ttcggcccct atttcgatgc gtccaacatc
      721 tgggggccgg tcagcggagt gaaaatatat gcaggggtaa ggtttttgct aaaacatgat
      781 aagttataaa ttccatttaa aaacaaagta ttatgaaaaa aataattgca ttaacaggaa
      841 tattggttct agccattagt atctcatatc ccactatggc tcaggaaaag aagaaaaact
      901 acgaggaagt tcagatccag acatcggcga tttgcgggat gtgtgaagac cggattgaga
      961 ccaatatttc gtttgagaaa ggcgttaaga aagttcaatt aaatgatgac accaaagtgg
     1021 tcacaatcgg ctatgatccg cggaagaccg ctccggataa acttcggaca gccatttcaa
     1081 aacttggtta tgatgcggat gataaaccgg cagattcgtt agcacacgca aagttaccgg
     1141 tttgctgcca gaaagggagc aagccgcact aaagagcttt gagggttgag ctggggaagg
     1201 gagatattct accgggggtc aggtattcaa acctttcact cgtggtatac ccggaataaa
     1261 atctttcagg taatagggct caaagtaaac ggtatcttca aaggctcgtc ggttgaatat
     1321 attttcggca aggtggacca tgtaggtggc cgaaagctga aaatcatcta ggaacctggc
     1381 atttggatgg ctggccagga gtggtttgca tttttcggcg ccatctccaa agaacagtac
     1441 cgggtgatct ttcatttctt caagaaatga tccttcgtga atgatttcag ccttaatctc
     1501 cctgacaaga ttcaggtcgc tatcatagag ggcgttatag acttccatcc ggcgggcgtc
     1561 gatcatgggg cagaaaaggg atggcgtccc aagggacgcc atcccttttg cgcccgcagc
     1621 catcgattcc agtgtactta tggcaattaa tggtttgcct gcgccataac atattccctt
     1681 tgccgccgat actccaatcc tcagccccgt ataagaacca ggccctttgc taacggcaac
     1741 agcatccaat tcggaaaatg caatacccgc cttcttcatc actgcatcga taaaaacagt
     1801 caaagtaacg gaatgactgt tcttttcccc cgattccatg agctcaacga cccggccatc
     1861 gtcggataag gcaaccgaac acacctgcgt cgccgtttct aaattgagaa tgagcggcat
     1921 aactaccctt gtgaaatgtt cctgaaattg atattgaaac agttgcttac ttctcctcct
     1981 ccgaatatag gtccttctta tctactacct ttataggatc accgtttttc agtcttttgg
     2041 atatggcacg gtaaggcgcg gtgatcacct cctgtccttc ttccacgcca cttttcactt
     2101 cgatgtaggt gatatcctgg atcccggttt taacctcctg cagcaacgct ttgccatcct
     2161 ttaccaggaa aatatattcc tttacctcgt ctttagtggc cgctaagtcg atattggcgt
     2221 cctcttcctt caccgttcct gtcgtatcgc tgcgtgtcgt taccgattga atgggaacag
     2281 ccagaatgtt gtaaaccgtc tcggtctgga tatccaccgt tgccgacatc cccggccgga
     2341 aaggagagcg gatggcgctt gcggtatcga tcatatcggt ataagattcg ggaaggatcc
     2401 ggatcttgac attgaagttc gtgatctggt cggcgctgac gcctgaagta ttggccgacg
     2461 ttgatatctc cgtgacgatg cctttgaatt tcttgtccag gtaagcatcg acctcgatct
     2521 ggcaggtgtc gaaaagcgaa accctgacaa tatcattctc atttacttcc acattgacct
     2581 ccatgctctg caggttggca atgcgcatga tctcggtacc ggctgaaaat tgcgaagctc
     2641 ccgtcacccg ttccccgatc tccacactca gtttggaaac ggttccgtcg ctgggtgaaa
     2701 ttattgtcgt tttgaacaga ttttccttcg cctcttttaa tgaagcttcg gcgctttcca
     2761 caccgtactg cgaacttttc aggctctgga atgccgcatc cttttctgct ttggcaactt
     2821 cataagatga tttggccgca tcaaaatcag catcggagat cactttctgc tcccataatg
     2881 ttttgctccg gttgaagcta agttctgcat tccggaattg cgcctccacc tgggccaccc
     2941 gggcctgggc attcgattcg ccggcttttt gcgaattcag cattgccatc atccggtcgt
     3001 agttggcccg gtaaatctcc ggatcaatct tggccagcag gtcgcctttt tttacctggt
     3061 cgccctcttt tacataaagc tcaataactt cacccgagat gtacgggctg atcttaatgt
     3121 ctttctccgg ctgtatcttg ccatttgccg aaaccatctc gataatggtc cgtttttcaa
     3181 ctttttcggc ggtgaccttg gtccctgtcc tgtcttttcc tttgttcatg gccaggataa
     3241 ccaggataat aatcaggata acccctccga ttaccaggaa gagatttctt tttttctgga
     3301 agaatttcat actgcttgaa ttaaggtatg cacaagaaag atcaaaagta gggctttttt
     3361 aaagatttta aagggcattt tagattttta taaatgatga tccaaattcc ttccaacctc
     3421 atcctctttt cgatataata cttttatctt tgatactcat cattatttcc aataacatga
     3481 gaataatatc cagatccttg ctgccggttt tatgtcttat catgctgaat tccctctcaa
     3541 taaatgccca gatccatgct cttgaggcca cacattacga gatcagcata acggatatcg
     3601 atttccagtc gggagccatc actgcttcga ccaccgtgag cctgacgcct gtctccgggc
     3661 ccgttgatga aataagcctg gaactgatcg accttgcggt gggagctgtc tggaccggca
     3721 accagccggt atccgatttt gcccagaccg acagcctgat catatttcca ctgccaacag
     3781 cgcttcagcc gggagatacc ctcgatgtca ggattgatta ttccggaaac ccttaccatg
     3841 aaggctgggg aggctttcat ttcgccggag aatatgcttt caacctgggc gtcggattcg
     3901 aaatcatccc gcataacctt ggaaagagct ggtttccctg tatcgacaat ttcaccgaca
     3961 gggccagcta tgatgtctat tgcaccctgc cggccggtaa gatggctgtc ggaggaggga
     4021 tccttgccgg gatctcaaac aatggaaacg gcaccacaac ctattactgg aagttgtccc
     4081 agcagatccc gacctacctg gcttcgcttg ccgccggcaa ttatgtggct gtttccgata
     4141 ccttcagcgg tatgaacggg caaatcccca tcgatatcta tgtcaggccg caggacgcta
     4201 actatgttgc cggcaccttt gctaacctga aagacattct gtcaatcttt gaagagtgca
     4261 tgggacctta ctcctgggac cgggtggggt acacatccac attgctcggg gcgatggaac
     4321 atgccaccaa tatctttgtt ccctatggaa ccatcaacgg ctctctggcc tatgaatcac
     4381 tgatggcgca cgagcttgcc catatgtgga tgggcgataa agtgacctgc tcttcggcgg
     4441 aagagatgtg gataaacgaa ggatgggcga cctttttcgg cctctattat gccctggctc
     4501 ttaaccacga taccaatgcc tttaaacagg gcatgcgggc caaccatgcc aaagtactcc
     4561 agtactgtca tacttcctct ggcgatggtt cctacttccc gttgaaccag atcccccagg
     4621 aatacaccta tggcatgtcg gcttacgaaa ggggctcaac cgtggcccag gcgctgcggt
     4681 tttacctcgg cgacagcctg ttctttgaag ccctgaccgg ctttatcgat gaatttgcat
     4741 ttgatgatgc ctcatcctat gatatgcgcg acttcctgac cggtttcacc gggatggata
     4801 tgaccggatt tttcgataat tatgttctga attcgggtac gccccattat tccatcgatt
     4861 ccttcgatgt atccaacagg gacgtagatg attacgatgt aaccgtttac gtgaaacaga
     4921 aacggaaagg gccggcattc actgggaatg gaaatgtcat ggaaataatg ttcttggacg
     4981 ggaactggaa ccgttattcc gataccattc actttaatgg tcaatcgggc tgttccgtca
     5041 aatcggtccc gttcctgcct tctgtcgtgc tggttgatcc ggaagagaaa atgtgtgatg
     5101 ctactaccga taactacaag atcattaaaa attcaggaaa ttactcattc gacaagacct
     5161 tcttcagcct ggagcaggta accctcaccg attcagcttt tgtgcaggtc actcacaact
     5221 gggcgccgcc cgacagtatt aaggatcctg actccggcct gcgcatctcc gattatcgtt
     5281 actggcgtat cgatggtatt ttccctgatg atttccaggc caggggcaag ttcttttact
     5341 cggtgggtga ccttctcgac aacaacctca ttcttacatc gtctgatacg gtcatcatcc
     5401 tttaccggac cggggctgcc gacgactggc acgagataac ctttgacaag atcggcccgt
     5461 ggaacatcgg gtatattttc gtcgatgata tccagccggg cgaatacacg cttgcagtca
     5521 gggaaaccgg tgtcggtatt gggagccata catgggaaca ggcagaaacc ctggctgtat
     5581 ttccgaatcc ttccggtggc agttttacca tttccacagt tagcgataaa ggcggagaac
     5641 tgagaatcta cagcgattcc ggaacgatgg tcaggtcgat gaccgtccac cccggcaaga
     5701 agaagacaaa atggacgccg gggaacattg cggccggcag ttactatgtt acattccatg
     5761 aagccgggcg gaatacatcc gaaagcgtta caattatcta ctcaccggga ttatgagaga
     5821 attgagtgcc ccttccggga gattgctttc gctggatgcc cttcggggct tcgatatgtt
     5881 ctggatcatc ggcgccgaag ggatcgtgca tgccctgaaa gaggccacag gctggggatg
     5941 ggcggtgtgg atgtcggggc aactcgaaca tgtggaatgg aacggattcc ggttttacga
     6001 tctgatcttt ccgttgtttt tattcctgag tggcgctacc atgcccttct cgctgacaag
     6061 aaagctggaa cagggtattc ctaaaaacaa gatctatctg caagtattga aaagaggatt
     6121 gattctcgtc atcctcggca tgatctacaa cgggctgttc cggcttgact tcgagaacca
     6181 acgctacgcc agcgttctgg cacagatcgg aatagcctgg atgctggctg cttttatatt
     6241 tctgaacaca agctggaaag gacaggcgat atgggttgcg gggattgtgc tggcgtactg
     6301 ggcggccatg aaacttattc ccgtgccggg attcgggccc ggtgtgctga cacgcgaagg
     6361 cagcctggcc acctatatcg accagatgct gttgccgggg aaaatgcatg aagggatctt
     6421 cgacccggaa ggaatcatgg taaaaatacc ggctacggca acggccttgc tcggcgcatt
     6481 gaccggatgg tggctcaaaa acaaatccac cggtgaatat gccaaagtgg ggctgatggc
     6541 tgctgccgcg gttttctttt tcgtcctcgc ctatctcatc ggactttcat ttccaatcaa
     6601 caaaaagctc tggtcgcctt cctttgtttt tcagacggca ggctgcagcg tgttactgct
     6661 ggccttattt tacctgttga ttgatatctg gaaattcagg aaatgggcat tcttctttat
     6721 cgtcatcggg ctgaacccga tcacgatata catggctacc gggatcatca atttctggca
     6781 gatatcggat tacttcctgg gcggcctggc aggtttatcg cctgagcact ggaagaacgt
     6841 atggttgatc accggaatgg tagcctttga atggcttttc ctttattttc tttaccggaa
     6901 aaggatatta ttaagagttt agc
//
LOCUS       DAOPNH010000184         6890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12532_length_6890_cov_5.803219, whole genome shotgun sequence.
ACCESSION   DAOPNH010000184 DAOPNH010000000
VERSION     DAOPNH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12532_length_6890_cov_5.803219"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1963
                     /locus_tag="PKZ74_09980"
     CDS             116..1963
                     /locus_tag="PKZ74_09980"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93127.1"
                     /translation="MNRIHRQSLFIKSFICCLLLIVSAGLRAVPEDGKTLTDLITRAE
                     SLVESDPGEAKSLLRIAAARSDPKRFPKQTSRINFLLGEASFYLDDPDSVIAYYLKAV
                     EIDVASGNDRTAEHINILGNLGYMYDLIDQKQIAIDFYQKALNIAREINQKDEIAANL
                     ANIAQLKTLQGFYEEAMLYMEEALAFDKESGDESIIAIDLNTIGRIYESMGMYAQAVS
                     YLEQALEIDLRLKNEDKVAIRYNSLGLVYKGWGKYNMALEYFGKALEIDQKLGREEKV
                     ALRQANIGSTLIESGEVDRAIAYLEESLDYFKTNDRPSYTATTLNDLGRGYFMKKDYA
                     KAEKLFLESANICRQYGFVRFRMNSLDYLSRLYRESGQYQKAYLSISEFNVLNDSIFN
                     AESQKKIAEFNAKYELDKEQQENELLRRDREISRKRHTVAILAFSLAGLVLALFLLAV
                     LVRLKTIQNRRLRAEKENERLRMDLEQKNKELTYNAMCIIKNNETVAKIAETLEDAIQ
                     AGQDERGINNLVRKLQNMEREKNWTEFEVRFTKVHEDFYRKLNERFPDLTPNEKKLCA
                     FLKLNMSTKDIAAITHQSVHSINVARTRMRKKLGIDGTDENLIGFLNSL"
     gene            complement(2065..2412)
                     /locus_tag="PKZ74_09985"
     CDS             complement(2065..2412)
                     /locus_tag="PKZ74_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM93128.1"
                     /translation="MYDLEERTEKLAIEVRNLIKILPKTISNVEDSKQLIRSSGSIGA
                     NYIEANESLSRKDFVMRIKISRKEAKESIYWLNIIQESNNLDEQTKIRCESLIKETGE
                     IRNILSAILKKFV"
     gene            2741..2893
                     /locus_tag="PKZ74_09990"
     CDS             2741..2893
                     /locus_tag="PKZ74_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93129.1"
                     /translation="MAGSKKKPATENLNEMLKRIRSENKALEKLIRALEIRTAKKGEK
                     IKTNES"
     gene            2898..6038
                     /locus_tag="PKZ74_09995"
     CDS             2898..6038
                     /locus_tag="PKZ74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPM93130.1"
                     /translation="MNFKSLFFIVLVFSGTSMGLRAQQGIAINTTGASPDGSAILDVS
                     STSKGILIPRLDNGQMSGMSNPADGLLVYNTTANCFFYFESTGPGTGAWRQLIDNANN
                     NTLYDIDGDSYLTFKYDGGHDDTLYMLFDETEKYRITAKAIEMLNNSRSVMIGEGAGQ
                     YMDLTGDKHNIYIGYHAGNTNQYGGANTAVGHYALSENTVHNNTAIGSSALKFNTTGS
                     YNTAIGAGSQMNGVSGSSNTSVGYQSMIYSKGVENVAIGKQAMFYDTTGAYNVAVGNN
                     ALMYSRSSSSNTAIGHGALNMSSHGNGNVAVGYESMYLDSAGSNNTSIGQRSLYNNKN
                     GSYNLALGAYAAYNSRSSTGNMAIGAYSLYYSQNTTNNAAIGYGAMEQLTEGTNNIAI
                     GSNALRYSENVSENTVIGMNAMMFNKTGSRNTVIGYYAMIFDSIGSGNCILGHNAAYA
                     NKKGNDNVAIGQAALMNNTIRGDLVAIGDSALYSNGMGIIESQSYTAACNTAVGSNAM
                     MNNSSGYNNTALGFKCLYNNTQGDGNTAVGYKGMYTNSSGAWNTSLGYMAMHNNTGGI
                     NNTAAGYTAMMNNTMGIGNTALGQAALKTNTEGYYNVALGNAAMLSNTLGTSNIGIGN
                     EALKSNTTRGHLIAIGDSALYRNGIGATEIQHATKNIAVGSKSLYYNSTGRSNTSIGF
                     EAMTNNNGGSENVAVGTQALYNNTSGAGNTAAGVSALLSNSGGYENTAFGYHALEDNN
                     GNYNSGFGSFALMNNASTGFNNTAVGAYALGLVTTGDNNTAIGYYTASGANFANSTFI
                     GASIDITTARTNISVLGYGVTNGECTGNNQVLLGNTSISQIRAMVGSITTYSDARIKS
                     DVSENVPGLDFICKLRPVTYNKDPEKLHQIWGTPAEQVERIDFTDARHEYIHRRDGLA
                     VIVEPHVERFDLLRVVEDGDRLLEMFLGEPAFVFGLEVQAVGDGVLEFLPAFLQQLHG
                     IGVGDADERFGGNELKAFAKSLVDELFEDLQIALVIFESVGDEVFHQCFGKIHVAFEI
                     AESDFGFDHPELRGVA"
     gene            6255..6725
                     /locus_tag="PKZ74_10000"
     CDS             6255..6725
                     /locus_tag="PKZ74_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93131.1"
                     /translation="MDVDEIAFLEKLVDRECEPAAHAEHRAIEIRPRPQVGDRAEELG
                     RVAFFLQREIFRHSAEQFDRNGLHFPSLTCGGRGDQFPAHLDGCAGAHLRDVFISGNL
                     RVRDDLEISQTGAVVEFDERKRLGIPPRPDPAGDVEIVRGFRAFQDVFDERPHA"
BASE COUNT         1930 a         1579 c         1699 g         1682 t
ORIGIN      
        1 accagtattt tgatcatttt gacaggattg tttttaacaa atttaatgcg aattatttta
       61 ttgatgaaca gatgatcaaa tttgattatt ttagaagtcg atatcgattc tggctatgaa
      121 taggatacac cgtcaatctt tgtttattaa gtcatttatc tgttgtttat tattaattgt
      181 ttccgcaggc ttacgagctg ttcctgaaga tggaaaaaca ctgactgacc tgatcacccg
      241 ggcagaaagc ctggtggaaa gcgaccccgg tgaagcgaaa tccctgcttc ggattgccgc
      301 tgctagatcg gatcccaaac gttttccgaa acaaacatcc agaataaact tcctcctggg
      361 tgaagccagt ttttacctgg atgaccccga ttcagtcatc gcgtactacc tgaaagcagt
      421 tgaaatcgat gtggcatccg gtaatgaccg gactgcggaa catatcaata tcctgggtaa
      481 cctgggctac atgtacgatc tcatcgatca gaagcaaata gccatcgatt tctatcaaaa
      541 agcgctgaat atagcacggg aaatcaacca gaaagatgaa atcgcggcca atctggccaa
      601 tattgcccag cttaaaaccc tccagggatt ttatgaagaa gccatgctct atatggaaga
      661 agcattggca ttcgataaag agtcgggcga cgaatccatc attgccattg atctcaacac
      721 gatcgggaga atctatgaat cgatgggcat gtatgcacag gctgtcagct atctcgaaca
      781 agcgctggaa atcgatctgc gacttaaaaa cgaagataaa gtggccatac gttataacag
      841 cctgggactg gtttataaag gatgggggaa atataacatg gcgctggagt atttcggaaa
      901 agcgctggaa attgatcaga aactggggcg ggaggaaaaa gtggccctgc ggcaggctaa
      961 tatcggcagt actttgatcg agagcggaga ggttgaccgg gctatcgcct acctggaaga
     1021 aagtcttgat tattttaaaa caaatgatcg tccttcttat accgctacta ccctgaatga
     1081 cctgggcaga gggtatttca tgaaaaagga ctacgctaaa gcagagaaac tctttctgga
     1141 aagtgccaat atctgccggc aatacggctt cgtccggttc cggatgaaca gcctcgatta
     1201 tctctccagg ctttaccgcg aatcgggtca ataccagaaa gcttacctct ccatttcgga
     1261 attcaacgtg ctgaacgact cgattttcaa tgccgaaagc cagaaaaaga ttgctgaatt
     1321 caacgccaaa tacgagctcg acaaggaaca gcaggaaaat gaactcctgc gccgtgaccg
     1381 ggagatcagc cggaaaaggc ataccgtcgc tatccttgct ttttcgctgg ccgggcttgt
     1441 cctggcgctt tttctcctcg cggtcctggt aaggctgaag actattcaaa accgcaggct
     1501 cagggccgag aaagaaaatg aacggctgag aatggatctg gagcagaaga acaaagagct
     1561 gacctacaac gctatgtgca tcatcaagaa taatgagact gtggcaaaaa ttgccgaaac
     1621 gctggaagat gcaatccagg caggacagga tgaaagaggt attaacaatc tggtccgtaa
     1681 acttcaaaac atggaaaggg agaaaaactg gaccgagttt gaggtacgtt tcacaaaggt
     1741 ccacgaagat ttctaccgga aactgaatga gcgcttcccc gaccttaccc ccaatgaaaa
     1801 gaagctttgt gccttcctta aactaaatat gtcgaccaaa gacattgccg ccattaccca
     1861 tcagagtgtt cacagtatca acgtagcccg cacccggatg cggaaaaagc tgggcattga
     1921 tggcacagac gagaatctta tcgggttttt gaacagcctt tgaaaattcc aaaattccaa
     1981 gcaccaagct ccaaacaaat tccaagcacc aaattccaaa acccaaacaa attcaaaaaa
     2041 caaatatcaa attcaaatat tgcttcaaac aaattttttc aagattgctg aaagtatatt
     2101 cctgatttca ccggtttctt ttattagcga ttcacacctg atcttagtct gctcatcgag
     2161 attatttgac tcttgaataa tgtttaacca gtaaatcgat tccttggctt cttttctgct
     2221 gattttaatt ctcattacaa agtctttgcg actaagggat tcattggctt cgatatagtt
     2281 tgcccctatt gatccggagg accgaatcaa ttgtttacta tcctcgacat ttgaaattgt
     2341 ctttggcagg atcttgatga gattcctgac ctcaattgcc agcttctcag ttctttcctc
     2401 taaatcgtac atataaatgt gataataatg gtttcaaatg ctttatccgg aaaatcataa
     2461 aaaatacccg caactgatat aaatatttta tcagaaatat ctaatatgtt gtatgtcata
     2521 tggttacaaa aggatagaat ttagatgaaa tgattgcttt cacccctgtt ttgatttttt
     2581 tagatttttt gaatttgttt ggagtttgga atttggtgct tggaatttgt ctggagtttg
     2641 gtgcttggaa tttggaattt ccccgtctgt atcaataaag tatcacgtta aaatttgcat
     2701 tttccatgtc ctgcctttta catttacgct ctgaattatt atggcaggat ccaagaaaaa
     2761 accggcgacc gaaaacctga atgaaatgct gaaacgcatc cggtcagaga ataaagcatt
     2821 ggaaaaactg atccgggctt tagagatcag gacggctaaa aagggagaga aaataaaaac
     2881 caatgaatca taaaaatatg aatttcaaat cgttattctt cattgtcctg gtcttttcag
     2941 gaaccagcat gggcctcagg gctcagcagg gtatcgctat caacactaca ggcgcatcgc
     3001 ccgatggctc agccatactg gatgtgagca gcacttccaa agggatcctg atcccacggc
     3061 ttgataacgg gcagatgtcc ggcatgtcga atcctgccga cggccttctg gtttataata
     3121 ctactgctaa ctgctttttc tactttgaat caacgggtcc tggcaccggg gcctggcgcc
     3181 agctcatcga caatgccaat aacaatacct tatatgacat tgacggcgac agctacctga
     3241 cattcaagta cgacggaggg catgatgata ccttgtatat gcttttcgat gaaacggaaa
     3301 aataccggat cactgcaaaa gcaatcgaaa tgctgaacaa cagccggtcg gtgatgatcg
     3361 gggaaggggc cgggcaatac atggacctga caggcgacaa gcacaatatt tatattgggt
     3421 atcatgcagg aaatacgaat caatacggtg gagccaacac ggctgtcggc cattatgcat
     3481 tatcggagaa tacggtgcac aataacactg caattggcag ctcggccctg aaatttaaca
     3541 cgacgggcag ttataatacc gccatcggcg ctggttcaca aatgaatgga gtatcgggtt
     3601 cttccaacac ctcagtgggt tatcaatcga tgatttattc gaaaggagtt gagaacgtag
     3661 cgatcggaaa gcaagctatg ttctatgata ccaccggagc ctataatgtc gctgtgggaa
     3721 ataatgcgtt gatgtattcg cgaagcagca gttccaatac ggctatcggc catggggcgc
     3781 tcaatatgag cagtcatggc aatgggaatg tggcggtcgg gtacgagtct atgtacctgg
     3841 attccgccgg ttcgaacaac acatcgatag gacagcgatc actttataat aataagaacg
     3901 gaagctataa tcttgccctg ggagcctatg ctgcttataa cagcaggagt agcacaggga
     3961 atatggctat cggcgcctat tcattatatt atagccagaa tacgaccaac aatgctgcta
     4021 ttggatacgg ggctatggaa caattgaccg aaggaacaaa taatatagcg atcggttcaa
     4081 atgcattgcg ctattcggaa aatgtcagtg aaaatacggt gatcgggatg aatgccatga
     4141 tgtttaataa gaccggatca agaaatacgg tcatcggata ttatgccatg atctttgact
     4201 ccatcgggtc cggcaactgc atcctgggac ataacgcggc ttatgccaat aaaaaaggca
     4261 atgataatgt ggccatcggc caggccgcct tgatgaataa taccataaga ggcgatcttg
     4321 tagctatcgg tgattcagcg ctttatagca acggaatggg cattattgaa tcacaatcgt
     4381 atacagcagc ctgtaacacg gcggtcggat ccaatgccat gatgaacaac tcatccggtt
     4441 ataacaatac tgccttaggg tttaaatgcc tctataacaa tacacagggc gatggcaata
     4501 ctgccgtggg ctacaaggga atgtacacca acagcagcgg ggcctggaat acatctttgg
     4561 gctacatggc catgcacaat aataccgggg ggatcaacaa tacagccgca ggttacacgg
     4621 ccatgatgaa taacaccatg ggaatcggaa atacggccct ggggcaagca gcgcttaaaa
     4681 ccaacacgga agggtattac aatgtagccc tcggcaatgc tgcgatgtta agcaacacgc
     4741 tcggaacctc caatatcggt atcgggaatg aagccttgaa aagtaacacc acccgcggcc
     4801 acctgatcgc catcggagac tcggcgcttt accggaacgg gatcggcgca accgaaatcc
     4861 agcatgccac aaaaaatata gcggtcggat caaaaagttt atattacaat tcgacaggca
     4921 ggagcaatac ttctattggc tttgaagcca tgacaaataa caatggcgga tcggagaatg
     4981 tggcggtagg aacccaggca ttgtataata atacgtcggg ggcagggaat acggcagcag
     5041 gtgttagcgc cttgctcagc aactctggcg ggtacgaaaa taccgccttt ggctaccatg
     5101 ccctggaaga caacaatggg aattataaca gcggattcgg gtcctttgcc ctgatgaaca
     5161 acgcatcgac cggatttaac aacactgccg ttggagctta cgcactggga ctggtcacta
     5221 ccggcgataa caatacggcc atcggatatt ataccgcttc cggtgcaaac tttgcaaaca
     5281 gcacatttat cggtgcctct attgatataa ctacagcacg gaccaatatt tcggtgctgg
     5341 gatacggcgt cactaacggt gaatgcaccg gcaacaacca ggttttgctt ggaaacacct
     5401 cgataagcca gatcagggcg atggttggca gtatcaccac ttacagcgac gcacggataa
     5461 aatcggatgt atcggaaaac gttcccgggc tggactttat ctgcaaactg aggcctgtca
     5521 cttacaacaa agacccggag aagcttcacc agatctgggg tacgccggcc gaacaagtag
     5581 aaagaattga tttcaccgat gcccggcacg agtacatcca tcgccgtgac ggtcttgccg
     5641 tcatcgttga gccgcatgta gaacggtttg atctccttcg ggtagttgaa gacggtgacc
     5701 ggttgcttga aatgttcctc ggtgagccag cgttcgtgtt cggactggag gttcaggccg
     5761 taggcgacgg ggtattggaa ttccttcccg ctttcctcca gcagcttcac ggcatcggtg
     5821 taggagatgc ggatgaacgg tttggtggaa acgaactcaa ggcgttcgcg aagtcccttg
     5881 tcgacgaact tttcgaagat ctccagatcg ccctcgtgat tttcgagagt gtaggtgacg
     5941 aggtatttca ccaatgcttc ggcaagatcc atgtcgcctt cgagatcgca gaaagcgact
     6001 tcgggttcga tcatccagaa ctccgcggcg tggcgtgagg tgttggaatt ttccgcgcgg
     6061 aatgtcggcc cgaacgtgta gatgttcgag agcgcgcagg cgaaggtttc gccttcgagc
     6121 tggccactga cagtcaggta agcgcgcttg ttgaagaagt cttcggcgtc cttggaaatc
     6181 ttgtcgtccg gcagtgtcgt gacgcggaac atctcaccgg caccctcgca atcgctagcg
     6241 gtgatgatcg gcgtgtggac gtagatgaaa tcgcgttcct ggaaaaactt gtggatcgcg
     6301 aatgcgagcc ggctgcgcat gcggaacacc gcgccataga gattcgtccg cggccgcaag
     6361 tgggcgatcg tgcggaggaa ctcggtcgag tggccttttt tctgcagcgg gaaatcttcc
     6421 ggcactccgc cgagcagttt gatcgaaacg gcctgcattt cccaagcctg acctgcggcg
     6481 ggagagggga tcaatttccc gcgcacctcg acggatgcgc cggtgctcat cttcgcgatg
     6541 ttttcatatc cgggaatctc cgcgtccgcg acgatctgga gatttcccag acaggtgccg
     6601 tcgttgagtt cgatgaacga aaacgccttg gaatcccgcc gcgtccggac ccagccggcg
     6661 acgtggagat cgtccgtggc ttccgcgcgt ttcaggacgt gtttgatgag cgaccgcatg
     6721 cctgatcgtt atggacgaag ggcggatttt gtcaaagccg cgcggtgaac atcgggagat
     6781 gtttgaaatg ctttttcgtt gcctttgcct ggagatgcca tcagtttccc agaaccgtga
     6841 aaagcttggc atcgtctcta cgcaaatccc ttacgcatga cggcttcatc
//
LOCUS       DAOPNH010000185         6880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12550_length_6880_cov_5.721026, whole genome shotgun sequence.
ACCESSION   DAOPNH010000185 DAOPNH010000000
VERSION     DAOPNH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12550_length_6880_cov_5.721026"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="PKZ74_10005"
     CDS             complement(<1..1601)
                     /locus_tag="PKZ74_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93132.1"
                     /translation="MKIKNIIAIFWFMILLINQAYPQIITVKQDGTGDFTAIQQAIIA
                     SQDGDTVLAWPGIYYENLDFNGKSLTLASLNLTTGDPTYIHTTVIDGNQQGRCVTMNS
                     NEQLAAIYGFTMRNGWERFGPHAISDNSGGGILAYEIGELKVTNCIIKDNQVNGGGGG
                     IYCKDLTLYLSGVFIFNNYAVSGGGGICLVVDGNIIFDSINRCNIYLNYSARGNDFAH
                     NVSHPIEVIVDTFTVMNPDIYYLSSTDMYGFQNGLITYDILHHKIEPVNGDLFVSPVG
                     SDSNSGTSPDDPLRSVSFALTKIVSDSLHPNTIHLANGVYSLSFTDEKYPLNIRSHIT
                     IEGEHQDSVFLDGDSIIYILKGNNEVSDFVFRNLTICNGNGIINTSIGVGLMRLYVNY
                     NALFENVSLTGGVGDLRSTFTLIGDNTTFKNVRIFENYGGFPHCAIHPLAFLPGVIVH
                     DTVEFINVKYFYNQPSPNPLYGLGDGISVGGTLEWDNSMTVNFIGCEFTDNVENFTGW
                     NATTCLIINENAKANLVNCTLVNNL"
     gene            complement(1777..5472)
                     /locus_tag="PKZ74_10010"
     CDS             complement(1777..5472)
                     /locus_tag="PKZ74_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93133.1"
                     /translation="MKTIFFKISWLIFTLINLVNGYSQINNSTLSDEVIYLDPDTCYP
                     GLANGSPSRDQGPETVWLNTGLPISGGIPYDIIYVEDEIDPLNSLIFIYTERKLLTYY
                     MFEYIWGPTIDLTEYGSQTLFKLTQPLLSNSREKHLAYNPVKHELYCLTQDLKILVID
                     PFSLVTAPIIREIVPNTDIGLLDWAILKYDPVSQYIFLGLCRYHDASEAELMAFNTTD
                     YTFEYSIHNGNLTDVSFNPNPYYSILYLSSNSEIQLRNTLDGSLINSYPMPCSMGVIF
                     NAYNESTGLNKTYCLPKSNIIVEDNALVFTGNNTNPQTKSMAKGNFGCGIYNALSDKV
                     YIGYTSIGTLPSGFISFDPDTENMTDHELTNDDILMDMANLEERVFISTRDHIYCCKN
                     GTNYPDYTLDSPDSYFYRITTTNFFNYPRIWATNLMDQSLQIANAPAEFINLEWYEPE
                     PFSGAALQGFFNHTDQKLYLYYGKRDGDHKFMYIYDPATADITSVRIDHNPTGVIVDE
                     VTNIIYFSRWLDNFIKRYDADGNSWLQPINLPEGFSFCNEMYISDRMLYCVTKINQGE
                     VDAARLLIYNLDEPLAEPEIIDIPCFSPSGYPIVAHYTVDNIGRIYISIRFVEKNQGR
                     VIRINEDFSMTDFGINFVEPDEILYDPLQHRILLKYLESDNISILDFEASPHSYQNFQ
                     PREGLAIIDMEIDPISNLVYFDYNDIQQNGYIDIFTTNGIFVKTVQVGYLALALKYNP
                     NNGLMYVHVPENHAADRKEQLWSIDPGTFDVYSMDLGQNEGHWRNAVYLTDLILDEEN
                     NKIYSVSAHGNIKIINCSNDQLTLQPGWNWLSYPRLDRSQGAPAPEEVLQQNYFSVGY
                     ENLLMTYYNYLWHPTNLASVTWEEGPAGWTYDGLTEVYSERGYKIHLLPEQSRILDLT
                     GTILDRASTFLLYAINPNWIGYFLPITQSPFDAIPEEFLNKITRITGQYWSCFKKDPL
                     PPLKSSGSLWGCACQQGRIELKYGDMITVNTVEEIGAFHWQYGGIFASVEPKAPSLYF
                     QYEEQPAYDAIFIELDTLNLPEEIGAFAGDSCIGATTVLPSDTMALICAYTEGFEGEE
                     ITFELLYPTKSARPRCRDYSVLNTTTGIREKRRIVAGENQPYFLVSLKSQENIPADEA
                     AFNVNVRPNPARDEFTVSYFSGEEALVELSLINTLGLPVRSWQRGIQGAGSYSIMIST
                     TGLPSGCYYLKVKAGNAVEIQKIIIIH"
     gene            5887..>6880
                     /locus_tag="PKZ74_10015"
     CDS             5887..>6880
                     /locus_tag="PKZ74_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HPM93134.1"
                     /translation="MSYIEFDKKQLVNLEFSLSRELIRTNRAGAYASRNVVGCHTRKY
                     HGMLVVPQPALDDDNHVLLSNLDETVIQHGAEFNLGIHKYPGGVYHPHGHRYLTHFST
                     TPIPHNIYQVGGVVLKREMLFATNADRYLIRWTLVDAHSPTRLRLRPFLAFRNAHRLS
                     KANINANTKYEKVPNGIRMKLYTGYSHLYMQFSKTPEYTHVPDWNYNIEYQMEMERGY
                     EYQEDLLVPGFFEIDIKKGESVVFCAGTEEIAPVSIKKAFDGEVKKRVPRDSFDHCLV
                     NAAQQFIVKKGKRTDIIAGFPWFGRWGRDTFIALPGLTLVLDDLKTFRSVIDTMV"
BASE COUNT         1994 a         1538 c         1390 g         1958 t
ORIGIN      
        1 cataagttgt tcaccagtgt gcaattcacc aggttggcct tcgcgttttc atttattatc
       61 aggcaggtcg ttgcgttcca gcctgtaaaa ttttccacat tgtcagtaaa ctcacatccg
      121 ataaaattga cggtcatgga attgtcccat tccagagtac caccgacaga tatcccatca
      181 cctagaccat ataatgggtt tggggaaggt tgattataaa aatattttac gtttataaac
      241 tctactgtat cgtgtacgat gacccccggt aaaaaagcca gggggtgtat cgcacaatgc
      301 gggaaaccac cataattctc aaaaatcctg acatttttga aagtcgtatt atcgcctata
      361 agagtaaaag tgctccggag atcaccgact cctcctgtaa gcgatacatt ttcaaaaagg
      421 gcattataat taacgtataa tctcattaaa ccaacaccga tggaagtgtt aataatcccg
      481 tttccattgc atatggtcag atttcggaaa acgaaatccg aaacttcatt atttcctttc
      541 agtatataga ttatagaatc tccgtcgaga aacaccgaat cctggtgttc cccttcgatg
      601 gtaatgtggc tacgtatgtt caacgggtat ttttcatcgg taaatgaaag agaatagacg
      661 ccgtttgcca ggtgaatagt gttgggatgc aggctgtcgg agacaatctt tgtgagcgca
      721 aatgaaaccg accgtaacgg gtcgtcaggc gaagtgccgc tgttggaatc actcccgaca
      781 ggactgacaa ataaatcacc gttcacgggt tcgatcttat ggtgcaagat atcataagtg
      841 ataagcccat tttgaaatcc gtacatatcc gtagatgaaa gataatagat gtcaggattc
      901 atcacggtaa aagtatccac aataacttca atgggatgtg agacattatg agcaaagtca
      961 ttacccctgg cagaataatt caaataaata ttgcacctgt ttatactatc aaatataatg
     1021 ttaccgtcaa ccacaagaca aattccgccg ccgccactta cagcataatt gttaaaaata
     1081 aatactccag ataaatacaa tgtcaaatct ttacaatata ttccccctcc accgccgttc
     1141 acctgattat ccttgattat acaattcgtg actttgagtt ccccaatttc gtatgccaga
     1201 attccaccac ctgaattatc ggaaatggcg tgcggaccga acctttccca tccattcctc
     1261 atggtaaatc catagattgc agcaagttgt tcgtttgaat tcattgtgac acatcttccc
     1321 tgctgattgc catcaattac tgttgtatgg atatatgttg gatcgcctgt agtcaagttc
     1381 agactggcca gggtaaggct tttgccattg aaatccaggt tttcataata gattcccggc
     1441 caggccagca ccgtatcccc gtcctggcta gcaattatgg cctgttgaat tgccgtaaaa
     1501 tcgccggttc catcctgttt gacggtgatg atctgtggat aagcctggtt gatcaacagg
     1561 atcataaacc agaaaatggc aatgatattt tttatcttca atgctcttat tttaatgagg
     1621 atttctcaaa aatacttaac cacaaaggaa cacaaagggt tgcacgaagg cgcacaaagg
     1681 ttttaatcct gtaatcaggt actttgcgcg cctttgtgat atattttgtg cgcctttgtg
     1741 gtttattaaa gacttttgat gcactcccgt taaatgttaa tgaatgatta taattttttg
     1801 tatttcaaca gcattaccag ccttgacctt caggtagtaa caacctgaag gcaaaccggt
     1861 ggtactgatc ataatcgaat agctccccgc gccctggatc cctcgttgcc agcttctgac
     1921 cggtaatccc aatgtattga tcaacgacaa ctcgaccaac gcctcttccc cggagaagta
     1981 cgatacggta aattcatccc tagccggatt tggccggaca ttgacattga atgccgcctc
     2041 atcagccgga atattttcct gtgatttcaa tgaaaccagg aaatagggct ggttttctcc
     2101 tgctacaatt ctgcgttttt cacgaattcc tgtggtggta ttcagaacag aataatccct
     2161 gcaccttggc ctggccgatt tggttggata cagcagttcg aaggtgatct cctcgccttc
     2221 aaatccttcg gtgtaagcgc agatcagggc catggtgtcg gagggcaata ccgtagttgc
     2281 accaatacag gaatctccgg caaatgcccc gatctcttcc ggcaggttca gggtatccag
     2341 ttcgataaag atcgcatcat atgccggctg ttcttcatac tgaaaataaa gtgatggggc
     2401 tttcggctcc acactggcaa atattccccc gtactgccag tgaaaagcgc cgatttcttc
     2461 aacggtattg acggtaatca tgtcaccgta tttcaactcg atccttccct gctggcaggc
     2521 acagccccag aggcttcccg aacttttcaa cgggggaagc gggtcttttt tgaaacaact
     2581 ccaatattgc cctgttatac gagtgatttt attaagaaat tcttcaggga tcgcatcgaa
     2641 cgggctctgc gttatcggga gaaaatatcc aatccaattc gggttaattg catataatag
     2701 aaatgtagat gcacgatcaa gtattgtgcc cgtcaaatcc aggatcctgc tttgctcagg
     2761 taacagatgg attttatatc ctcgttcact gtaaacttct gttaaaccat cgtatgtcca
     2821 accagcagga ccttcttccc atgttacaga tgccaaatta gtcggatgcc ataaataatt
     2881 gtagtatgtc attaacaaat tctcataacc aacagaaaag taattctgtt ggagcacttc
     2941 ttccggagcc ggagcgccct ggctgcggtc gagtcgcgga taagagagcc agttccagcc
     3001 gggttgcaga gtgagttggt cattactgca attgatgatt ttaatatttc catgagcgct
     3061 gaccgaatag attttgttat tttcttcatc caggatcaaa tcagttaaat agacagcatt
     3121 tctccaatgg ccctcgtttt gaccaagatc cattgaatag acatcaaatg tacccgggtc
     3181 tatgctccat aactgttcct ttctatcagc ggcgtggttt tcaggaacgt gaacatacat
     3241 aagaccattg ttaggattat atttcagagc caatgccaga tatcccacct ggacggtttt
     3301 cacgaatatg ccattggtag taaagatatc gatataccca ttctgttgaa tatcattata
     3361 atcaaaataa actaaatttg aaatgggatc gatttccata tcaataatag caagcccctc
     3421 acgtggttga aaattctgat aggagtgagg ggaagcttcg aaatctaaga ttgagatatt
     3481 gtccgattcg aggtacttga gtaaaattct atgttgtaat ggatcataaa gtatttcatc
     3541 aggttccaca aagttgatcc caaaatcagt catgctaaaa tcttcattga tcctgatcac
     3601 tcttccttgg tttttctcta caaatcgaat agaaatataa atcctgccaa tgttatcaac
     3661 ggtgtaatgt gctacaattg gatatccgct tggactgaag cacggaatat caattatttc
     3721 cggctccgcc agtggctcgt ccaggttata aataagcaag cgggccgcat ctacttctcc
     3781 ctgattaatt tttgtaacac aatataacat cctgtctgat atatacatct cattacagaa
     3841 tgaaaatccc tccggcaaat taataggttg caaccaggaa ttgccgtcag catcatatct
     3901 ttttataaaa ttatccagcc atcttgagaa ataaataata ttggtcactt catccacaat
     3961 cacaccggtt ggattatgat caatcctgac tgacgtaata tctgctgttg ccggatcata
     4021 gatgtacatg aatttgtggt ctccatcccg ctttccgtag taaaggtaaa gcttttgatc
     4081 cgtatgatta aaaaatcctt gcagtgccgc tccactaaaa ggctccggct cataccattc
     4141 caaattgata aattcagcag gagcattagc aatttgtaat gactgatcca tcaaatttgt
     4201 agcccaaatg cgagggtagt tgaaaaaatt ggtggttgta atacggtaaa aatagctgtc
     4261 cggcgaatct aatgtataat ccggatagtt tgtgccattt ttacaacaat agatatggtc
     4321 ccttgtgcta ataaaaaccc tttcttcaag gtttgccata tccataagaa tatcatcatt
     4381 tgttaattca tgatctgtca tgttctctgt atcagggtcg aatgatatga atccactcgg
     4441 aagagttcct atgctggtgt aaccaatata tactttatcg gaaagtgcat tataaatacc
     4501 acatccaaaa ttaccttttg ccattgattt cgtctgtgga ttggtattat tccctgtaaa
     4561 gaccagtgca ttatcctcta caataatatt gctcttcggc aaacagtaag ttttattcag
     4621 gcctgtactt tcgttgtatg cattaaatat tactcccatt gaacaaggca ttggataaga
     4681 atttattaaa gatccatcca gtgtatttct taactggatt tcactattag acgataagta
     4741 aagtatagag taatatggat taggattaaa agaaacatca gtaaggtttc cattgtggat
     4801 ggaatattca aaagtataat ccgttgtgtt aaatgccatt aattctgctt ctgaagcgtc
     4861 atgataccgg caaagaccca ggaatatata ttgcgaaaca ggatcgtact taagaatagc
     4921 ccagtccagc aacccaatat cggtgtttgg aacaatctct ctgattatgg gtgctgttac
     4981 cagagaaaaa ggatcaatta caagaatttt taaatcttga gtcaaacaat ataattcatg
     5041 tttgactgga ttataagcaa gatgtttttc ccttgaatta gaaagcagcg gttgggttag
     5101 tttaaaaagt gtctgtgaac catactcagt caggtctatt gtcggccccc atatatactc
     5161 aaacatataa taggttaaca atttcctttc tgtatagata aatatgagag agtttaaagg
     5221 atctatctca tcctccacgt aaattatatc ataaggaatt cctcctgaaa taggcaaacc
     5281 tgtattcagc caaaccgttt caggtccttg atcccgggaa ggactgccat tcgctaatcc
     5341 cgggtaacag gtatcaggat ccaggtatat cacttcatcg cttaatgtgc tgttattgat
     5401 ctggctgtat ccgttaacta gatttatcaa cgtaaatatc agccaggaaa tcttgaaaaa
     5461 tattgttttc atggttttat aaaattaaat gaattattcg attaattaga actggcttca
     5521 gttagtcagt taattatgga ggaaatatct tttccggaag aaagattact ctctctctct
     5581 ctctctctct ctctacggaa atacgacctc aggcaaaacc gtatcatcgg gattaattga
     5641 attgcttaaa agatcaatgg ttggggggcg ggacaaatat aataaaagtc gataggaaag
     5701 tcaagagggg ttgataactt tcgtcagtga gactccatct caattaaggt acgccatttc
     5761 acaaaatttg ttaaatggca ttggtattgt gaaatgcgga atgaaaattc ttaaaacttt
     5821 ccttaccttt gcaggccggt tcttgcctcg gtccaattgc agaaaaatat tgatttataa
     5881 agccctatga gctatatcga atttgataaa aaacaactgg tcaatcttga attttcactc
     5941 agccgtgaac tgatccgtac caaccgcgcc ggcgcctatg ccagccggaa tgtggtcgga
     6001 tgccataccc ggaagtacca tggaatgctc gtcgtgccgc aaccggccct cgacgacgac
     6061 aatcacgtgc tcctctcaaa cctcgacgag acggttattc agcacggcgc cgagtttaac
     6121 ctgggtatcc ataaataccc cggcggggtg taccatccgc acggccatcg ctacctgacc
     6181 cacttctcca ccacaccgat accccacaat atctaccagg tcggcggagt cgtcctgaaa
     6241 cgggaaatgc tgttcgctac caatgccgac cgatacctca tccgctggac actcgtcgac
     6301 gcccactcgc cgacaagact ccgcctgagg cccttcctgg cattccgcaa tgcgcaccgc
     6361 ctcagcaaag ccaatatcaa cgccaataca aaatacgaaa aagtgcccaa cggcatccgg
     6421 atgaagctct ataccggcta ttcgcacctt tatatgcaat tctccaaaac cccggaatac
     6481 acccatgtac ccgactggaa ctacaacatc gagtaccaga tggagatgga aagaggctac
     6541 gaataccagg aagacctgct ggtacccggc tttttcgaga tcgatataaa gaagggggag
     6601 agcgtggtgt tttgcgccgg taccgaggaa atcgccccgg tctcgatcaa aaaagcgttc
     6661 gacggcgaag tgaaaaaaag ggtgcctcgc gacagtttcg accactgcct ggtcaacgcc
     6721 gcccagcagt tcatcgtcaa aaaaggaaaa cgtaccgata tcattgccgg gttcccctgg
     6781 ttcggacgct ggggcaggga tacgtttatc gccctgccgg gcttaacgct ggtgctcgac
     6841 gacctgaaga ctttccggtc ggtgatcgat accatggtcc
//
LOCUS       DAOPNH010000186         6841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12640_length_6841_cov_5.192160, whole genome shotgun sequence.
ACCESSION   DAOPNH010000186 DAOPNH010000000
VERSION     DAOPNH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12640_length_6841_cov_5.192160"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKZ74_10020"
     CDS             complement(<1..657)
                     /locus_tag="PKZ74_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPM93135.1"
                     /translation="MLSVVIIAIVIALLFDFLNGMNDAANSIATVVSTKVLPPLVAVV
                     WAAFFNFAAVFFFGVHVATTIGKGIVEPAIINEFVILAALVGSVVWVYVCTNLGLPIS
                     VSHALIGGLIGPALVIGGKSVLVSSGIIKVALFIVLSPLIGLILGYFLMILTMLVFHW
                     TSPRKVDHLFRGLQLVSSAIFSIGHGTNDAQKTMGIISVLLFSTGRLGPEFHVPTWVI
                     I"
     gene            complement(668..1315)
                     /locus_tag="PKZ74_10025"
     CDS             complement(668..1315)
                     /locus_tag="PKZ74_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPM93136.1"
                     /translation="MSINTFFQKLVPKDKKFFPMFESQADLIVVAAGKLNEIFITNDP
                     ERHAVLIREIKDLENQGDEVAHQIFDELDKTFITPFDREDIHQLTSTLDDVLDFINGS
                     SQRIRLYRLKSFPAEFVRFSSVLLSGANEIRSAVRELYNLKRPEMIRQACIRVNEVEN
                     QADDLYHFVISDLFENEKDAIELIKKKEVLQTMERASDRMEDVADVLKTIIIKLA"
     gene            complement(1360..2811)
                     /locus_tag="PKZ74_10030"
     CDS             complement(1360..2811)
                     /locus_tag="PKZ74_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPM93137.1"
                     /translation="MKRLNFRLLVYLAGLILLIESGFMMACIPVSLIFSGGDFRALAL
                     SAGITGLFGAILFFYYRKRVRKTPGIREGFLAVTMTWLLISVFGALPYMIHGSIPNPV
                     DAWFETVSGFTTTGASILTDIEAVPKGLLLWRSLTHWMGGMGIILMVIVVLPSLKVGG
                     VYLFSAEASKISLDDIKPRVVDTAKRFGIIYIALTLIEIVLLTLGGMPVFDSVCHSFA
                     TIATGGFGTKNTSITNYSVYIQYVIIAFMFLSGINFTLHFLVSKGEIKKALRNEELRF
                     YTQVTLAITLIIFLILYFNGTSLHKAFRDSLFQVVSIITCTGFATADYLLWPAAGWFM
                     IFLTMFVGASAGSTGGGIKVIRHLLMVRNLRMGLKTFLHPRVIYGVKYQGNLVSEELL
                     RSVIAFYIWYLIIFFFSSLLMMIIGVDFLTSIGGVATSMGGIGPGLGTVGPASNFHHL
                     PSLAKILLTFDMIIGRLEIYAFLVLFSPAFWKR"
     gene            complement(2876..4168)
                     /locus_tag="PKZ74_10035"
     CDS             complement(2876..4168)
                     /locus_tag="PKZ74_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93138.1"
                     /translation="MLLSNLALTAVVGPSVVPSIGIIRVEFILFGLTLIGVALFHKKT
                     MYVALTGLVVVLLFKLIFDPAFDFGHYILGGHGEEGEWRVLLNLMGLLFGFAILAKHF
                     EESRVPEILPKFLPDDWKGGLVLLVMVFILSAFLDNIAAAMIGGTIAMVVFKGKVHIG
                     YLAAIVAASNAGGAGSVVGDTTTTLMWIDGVEPVWVLHAYVASFAALIIFGIFGSIKQ
                     HKFQPIVKDNNPGIKIDWTKIFIVALILVGAIATNVLFDFPALGVWIAILLGALISKT
                     PWFELKHAWQGTIFLMSLVMIASMMPVEELPPASWQTAFILGFVSAVFDNIPLTKLCL
                     EQGGYDWGVLAYTVGFGGSMIWFGSSAGVALSNMYPQAKNTVNYIKHSWFVIVAYVVG
                     FFVLLWVIGWEPHAPHKKGADSGNPDLKPVPEQSIHDH"
     gene            complement(4349..5647)
                     /locus_tag="PKZ74_10040"
     CDS             complement(4349..5647)
                     /locus_tag="PKZ74_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPM93139.1"
                     /translation="MVLANIVTKAAESAAGVPSLGPIRVEFILFGLTLIGVAIFHRKT
                     MYVALTGLVAVLIFKFIFDPAFHFGGHIFGSAGHEGEWRILLNLTGLLFGFAILAKIF
                     EDSRVPDVLPGLLPDDWKGGLLLLFIVFILSAFLDNIAAAIIGGTIAMVVFKGKVHIG
                     YLAAIIASSNAGGAGSVVGDTTTTLMWIDGVSFNWVLPAYVASFSGFLIYGIVGSMQQ
                     NRFQMIIKDIQPGTTIDWWRIFIVALILVGTILTNVFFDFPALGVWIAILIGSLVRRP
                     PWKELKHAWQGTVFLVALVTIASLMPVEELPPASWQSAFILGFVSAVFDNIPLTKLCL
                     DQGGYDWAILAYAVGFGGSMIWFGSSAGVALSNIYPKIRNSGKYVMNGWHVIVAYVIS
                     FFVMLAILGWNPHEPHKKEHEPGTSSSINMTLNDNPDVSH"
     gene            complement(5728..>6841)
                     /locus_tag="PKZ74_10045"
     CDS             complement(5728..>6841)
                     /locus_tag="PKZ74_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93140.1"
                     /translation="VNPEVTTTYTVTVNDGFNVSQSQATVQVDPLPVPFAGDNQVIVY
                     GTPTQLHGTASQGSGNYSFHWEPAYKLIDPDSENPHTVNLYETTVFTLSVTDLETGCP
                     CEAVDAVSVMINGSALSVNPTALPNEICLGDTVQLFSSSGGGTGIYSHTWTSLPEGFN
                     SSEADPLVVPGNSMTYLVDVWDGYNWASGSVEVKVNPNPVVHMGNDTTVCVFDTITLD
                     AGPDGASYLWFNGATGRYISISSTGIGYEARQVSVEVASDKGCMTRSSKTINFDFTAC
                     SGVEDPEGHPSFRIFPNPGNGVITFEFSKLSGPASLIIFDSFGRKILYDEFRCTSQEN
                     YKKVFDISFNSRGLYLIRLMISDNVYLFKYVLNRPG"
BASE COUNT         2066 a         1679 c         1478 g         1618 t
ORIGIN      
        1 tattataacc catgtcggca catgaaactc cgggcccagc cggcccgtgc tgaacagcag
       61 cactgaaatg atacccattg ttttctgtgc atcattggta ccgtgtccga tactgaaaat
      121 cgccgaagaa accagctgca aacccctgaa caggtgatcc actttcctcg gggaggtcca
      181 gtgaaaaacc agcattgtca ggatcatcag gaaatagccc aaaatcaggc cgataagggg
      241 tgataaaacg atgaacaagg caactttaat gataccggaa gagaccagga cagatttccc
      301 gccgatgaca agagccgggc ctattaatcc gccgatcagg gcatgagata cactgatcgg
      361 caggcccagg ttagtgcata catacaccca gacaacggat ccgaccagtg cggcaaggat
      421 aacaaactca ttgatgatgg ccggttcgac gattcctttg ccaatcgtgg tggccacatg
      481 cacaccaaag aaaaacactg cggcaaagtt gaaaaaagcc gcccatacaa ctgccaccag
      541 cgggggtagc acttttgtcg aaacaactgt cgcaatagaa ttggctgcat cattcatccc
      601 gtttaggaaa tcgaagagca gggcgatgac aatggcaata atgacaactg aaagcatgtg
      661 atccggttta agccagttta attatgatgg tttttaaaac gtccgcaacg tcttccatcc
      721 ggtcgctggc ccgctccatc gtttgcagaa cctccttctt cttgatcagt tcgatagcat
      781 ccttctcatt ctcaaaaaga tctgaaatca caaagtgata aaggtcatcc gcctggtttt
      841 ccacttcatt tacccggata caggcctgcc tgatcatctc gggccgttta agattataca
      901 attcacggac cgcgcttcgg atctcgttgg ctccacttaa taaaaccgaa gaaaaccgca
      961 caaattcggc gggaaatgat tttaaccggt ataacctgat tctctggctg gagccattga
     1021 taaagtcgag gacatcatcg agggtcgagg taagctggtg tatgtcttcc ctgtcgaaag
     1081 gcgtgataaa agttttatca agctcatcaa agatctggtg agccacttca tccccctgat
     1141 tttccagatc ttttatttca cggatcaata cagcatgtct ttcaggatca tttgtgataa
     1201 atatttcgtt gagttttcct gcagccacaa caattaaatc ggcctgggat tcaaacatgg
     1261 gaaagaactt cttatccttt ggtaccagtt tctgaaaaaa tgtattgata ctcatattct
     1321 tggtttttag tttctttgag ggttttcttt aaataaatat caccgtttcc agaaggccgg
     1381 tgaaaataga acaagaaaag catatatctc caaccggcca ataatcatgt caaatgtaag
     1441 taaaatctta gctaaagaag gaaggtggtg aaaattactc gccgggccca cagtacccaa
     1501 acccgggcct atgccgccca tgctggttgc cactccaccg atcgatgtaa gaaaatcaac
     1561 cccgatgatc atcatcagta atgaactgaa aaagaaaatg atcaggtacc aaatgtaaaa
     1621 agcgatgacc gatctcaaca gctcctctga tacaagattc ccctggtatt ttacgccata
     1681 aataaccctt gggtgcagaa atgtcttaag acccattctc agattcctaa ccatcagaag
     1741 gtgccggatg accttgatcc ctccccctgt agatccggca gaggccccga caaacatggt
     1801 caggaagatc ataaaccagc cggctgccgg ccagagcaga tagtcggccg tagcaaatcc
     1861 tgtacaggtg atgatcgaca ccacctggaa caaggaatcc cggaaggctt tatgaagaga
     1921 ggttccattg aaataaagaa tcaaaaaaat tatcagtgtg atggccagag tgacctgagt
     1981 ataaaacctc aattcttcat ttctcaatgc tttctttatc tcccctttcg acactaaaaa
     2041 gtgtagagtg aaattaattc ccgaaaggaa catgaaagcg ataataacat attgaatata
     2101 aacagaatag tttgtaatac tggtgttctt tgtcccgaat cctcccgtgg cgatcgtcgc
     2161 aaatgaatgg cagacactgt cgaaaaccgg catacctccc aaggtcagca aaacaatttc
     2221 gattaatgta agagcaatat aaataatacc gaatcttttg gcagtatcta ctaccctggg
     2281 tttgatatcg tccaggctga tcttggaagc ttcggcgctg aaaagataaa ctcccccgac
     2341 tttcagggaa ggcagtacca cgataaccat caggatgatc cccatccctc ccatccagtg
     2401 ggtcaggctt ctccagagaa gcaaaccttt gggcaccgcc tcgatatcgg tgagaataga
     2461 ggcgccggta gtggtaaaac cggaaacagt ctcaaaccag gcatccaccg gatttggaat
     2521 cgaaccatga atcatataag gaagtgcacc gaatacggag atcagaagcc aggtcatggt
     2581 gacagcaagg aatccttccc tgatcccggg agtctttctt accctcttcc ggtaataaaa
     2641 gaataaaatc gcaccaaata atcctgtaat cccggccgat aaagccagtg ctctgaagtc
     2701 cccaccggaa aaaatcaggg aaaccggaat gcaagccatc ataaagccgc tttcaatcag
     2761 caggatcagg ccggccaaat agaccagcaa ccggaaattc agccttttca tcggtcagac
     2821 gacccgttat tctgagaata ggcgttaaaa agcttttata aaaacaacgt tctttttagt
     2881 gatcatgaat gctttgttcc ggaacaggct taagatccgg atttcccgag tctgcgcctt
     2941 ttttatgcgg agcatggggt tcccaaccga tgacccatag taagacaaag aatccaacga
     3001 cataggcaac gatcacaaac cagctatgtt tgatataatt tactgtattc ttggcttgag
     3061 ggtacatatt cgacagggca acgccggcgg aagaaccaaa ccagatcatc gaaccgccga
     3121 atccaacggt ataagccaga acaccccagt cgtacccgcc ttgctccagg cagagttttg
     3181 tcagcgggat gttatcaaac acggcggaga caaagcccag gatgaaggcg gtttgccagg
     3241 aagcgggagg cagttcctcg acaggcatca tggaagcgat cattaccagc gacatcagaa
     3301 agatggtgcc ctgccaggca tgtttaagct caaaccaggg tgttttgcta atcaatgccc
     3361 ctaacagaat agcaatccag acgcccaggg caggaaaatc aaataacacg ttcgtagcaa
     3421 tggctccgac cagaataagt gcaacaataa aaattttcgt ccagtcgatc ttgattccgg
     3481 gattattatc ttttacaata ggttgaaatt tgtgctgttt tattgagcca aaaatgccaa
     3541 atattataag tgctgcaaac gatgccacat atgcatgtaa tacccatact ggttccacac
     3601 cgtcgatcca catcaaggtc gtcgtcgtgt cgccgacaac acttcctgct ccaccggcat
     3661 tactggcagc cacaatggca gccaggtatc caatatgtac ctttccttta aaaacaacca
     3721 tcgctatggt tccgccaatc atggcggctg caatgttatc cagaaaggca gaaaggatga
     3781 aaaccattac cagcaggact aacccgcctt tccagtcatc aggaaggaat ttgggcagaa
     3841 tctcggggac tcttgattcc tcgaaatgct ttgccaggat ggcgaaaccg aacagcaatc
     3901 ccatcaggtt cagtagtacc ctccactcgc cttcttcacc gtgtccgccc aggatatagt
     3961 gcccaaagtc aaacgcagga tcaaaaatga gtttaaataa caaaacgacc accagccccg
     4021 tcaaggccac atacatcgtc tttttatgaa agagggccac gccgatcagg gtcaggccaa
     4081 acaggatgaa ttcaacacga ataattccga ttgaaggtac cacagatgga ccaacaactg
     4141 cggttaaggc aagattgctc agcagcataa gtcaaaattt caataaagta attggataaa
     4201 tgctttctga tgtttttgac aggagaaatc ggatgcaaaa atagagtgga atattaatct
     4261 cttttacaaa acaggtaaaa aatatttttc agcaaataaa attcgtaatg acttctataa
     4321 cagtatatta acttacctgt aacagaattc aatggctgac atccggatta tcattcagcg
     4381 tcatattaat ggaggaggac gttccgggtt cgtgttcctt cttatgcggc tcatgggggt
     4441 tccaacccag tatagccaac atcacaaaga aactgatcac ataggctacg atcacatgcc
     4501 acccgttcat cacatattta cctgaatttc tgatttttgg ataaatattt gacaaagcca
     4561 cgcctgcaga tgagccgaac catatcatcg accccccgaa acctacagca taggcaagaa
     4621 tagcccagtc ataccccccc tggtcaaggc agagtttagt aaggggtatg ttatcaaaaa
     4681 cagcagatac aaatccaaga atgaaagccg actgccagga tgccggcgga agttcttcaa
     4741 caggcatcaa agaggctatc gttaccagag ctaccagaaa cactgttcct tgccacgcat
     4801 gttttagctc tttccatggc gggcgtctca ccaggcttcc gatgagaatg gcaatccaaa
     4861 cccccaatgc cggaaaatcg aaaaatacat tcgtcaatat cgttccaacc agaataagcg
     4921 ctacgataaa aatccgccac cagtcgatcg tggttccggg ttggatgtcc ttaataatca
     4981 tctgaaaccg gttttgttgc attgagccga caataccgta tatcaagaaa cctgaaaatg
     5041 aagccacata agccggtaaa acccaattaa agcttacgcc gtcgatccac atcagtgtag
     5101 ttgttgtatc gcccactacg ctgccagcgc caccggcatt gcttgaggct atgatggcag
     5161 ccaggtaccc tatatgcacc ttccctttaa atacaaccat tgcaatggta cctccgatga
     5221 tagctgcggc aatattatcc agaaatgccg ataggatgaa tacgatgaac aaaagcaata
     5281 atcctccttt ccaatcgtcg ggaagcaatc ccggcaaaac atccggcacg cgtgaatctt
     5341 caaaaatctt ggcaaggatc gcaaatccaa ataaaaggcc ggtcagattc agcaatatcc
     5401 tccattctcc ttcatgcccg gccgatccaa agatatgacc tccaaaatgg aaagccggat
     5461 cgaatatgaa tttaaatatc aaaacagcca caagtccggt taacgctacg tacatggttt
     5521 ttctgtgaaa gatggctacc ccgatcagag tcaggccaaa taagatgaat tcaacccgga
     5581 tcgggccgag agatggaacc ccggcagcgc tttcagccgc cttagtaact atattcgcta
     5641 ataccatacg tatctgattg agcaggcaaa gatagaaggg aaattattct ggtgaaagat
     5701 tgttgcagaa attcaaggta ataatggtca tcctggtcgg ttaagaacat atttgaataa
     5761 ataaacgttg tcgctgatca ttagcctgat cagatacagt cccctggagt tgaatgaaat
     5821 gtcaaatacc ttcttataat tttcctgact tgtgcaccta aactcatcat acaaaatctt
     5881 tctgccgaag gaatcaaaaa tgattaagct ggccggtccc gacaatttgg aaaattcgaa
     5941 agtgatcaca ccatttccgg gattaggaaa tatccggaac gatggatgcc cctccggatc
     6001 ttcaactccc gaacaggcgg taaaatcaaa atttattgtt ttagacgacc tggtcataca
     6061 tcctttatcg gaggctactt caacggaaac ctggcgtgct tcatacccga taccggtgga
     6121 gctgatgctg atatagcgtc cggtcgcccc gttaaaccac aaataggaag ccccgtcggg
     6181 accggcatca agcgttatgg tatcaaaaac gcacaccgta gtgtcattac ccatatgcac
     6241 aaccgggttc ggattaacct tcacttccac cgatcctgat gcccagttat agccatccca
     6301 tacgtctact aaataggtca tactgtttcc aggtacgact agcgggtcgg cttccgacga
     6361 gttaaaccct tcgggtagcg atgtccaggt atggctataa atgcctgttc cacctcctga
     6421 ggaagagaaa agctgaacgg tgtcacccag gcagatttca ttcgggagtg ctgtcgggtt
     6481 tacacttaga gcagaaccgt tgatcatcac ggaaaccgca tccacagcct cacacgggca
     6541 tccggtctcc aggtcggtca ccgacagggt aaatacagtg gtctcataca aattgaccgt
     6601 atgcgggttc tctgagtcgg gatcaatcag tttgtaagcc ggttcccagt gaaatgaata
     6661 atttcctgag ccctggctgg ccgtaccgtg caactgtgtg ggcgttccgt atacgatgac
     6721 ctggttatca ccggcaaaag gaacgggtaa aggatcaacc tgcacggtag cctgggattg
     6781 tgagacatta aacccatcgt ttacagtcac ggtataagtg gttgtcacct ccggattaac
     6841 c
//
LOCUS       DAOPNH010000187         6820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12695_length_6820_cov_4.987288, whole genome shotgun sequence.
ACCESSION   DAOPNH010000187 DAOPNH010000000
VERSION     DAOPNH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12695_length_6820_cov_4.987288"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1484)
                     /locus_tag="PKZ74_10050"
     CDS             complement(201..1484)
                     /locus_tag="PKZ74_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM93141.1"
                     /translation="MRISIEIFVLLICSLSNAQSLTKRALFLGNSYTYTNNLPQMVAD
                     VAVSAGDTLLYDSNMPGGYTLEGHSTNLVSLEKIAEGNWDYVVLQEQSQLPSFPISQV
                     ETEVFPYARILDSIINAENPCAETAFFMTWGRKNGDESNCSWWPPVCTYAGMDSLLNL
                     RYCMMADSNQAIVSPVGAVWKYIRQHDPSIEQYQADGSHPSVAGTYAAACSFYASFFR
                     KDPESITFDAALPATDAATIRAAAKTVVYDSLLTWHIGEYDPIAGFSYLDSEELQITF
                     INNSLYATEFQWDFGDGGTSYIMNPTHSYASPGTYEVSLVAGACGISDTIFQTIDLEG
                     VGISESGKSIDFTISPNPASTYFTLKIADFRGQMELTLHSFTGLEIGHYLLVSPRTVI
                     GTGQLPEGVYFARLMREHGGMVTQERVKLIIRRNR"
     gene            1625..1801
                     /locus_tag="PKZ74_10055"
     CDS             1625..1801
                     /locus_tag="PKZ74_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93142.1"
                     /translation="MRADRISVTNAAGQVLFEQRIKEGSVIESVDISDFPVGVYFVKT
                     ISKDHVSVFRFQKR"
     gene            complement(1851..3695)
                     /locus_tag="PKZ74_10060"
     CDS             complement(1851..3695)
                     /locus_tag="PKZ74_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93143.1"
                     /translation="MKNPVYYLLIILTILTARNKNEENSNWTEEDQAYYEHILELQNQ
                     GTDNFYVWLDIMDSLEAVDQLQQFFLGDTAVVSAVVGSQGIAIDYKNGMRGGIMLNPE
                     DFYGEDKSMPAGGHTSDKLPGELKSIVNNSKMIFLNPSNWERTELTDMIIPKNTEDLE
                     RVGYELKKVYKDRDATLDRFCELGGYGIIRIYSHGWAHPTKWDVVETYLQTGESVSNG
                     TTRKYGDDIKEGKIAIFNVKNVNSQLDPSPVYFISKDFITSHNDFSKDTVFFYGGFCF
                     SFLGTWPLIEESFAKGTYMGFSWRVRTNFNCNLGTAAIEYLSDTAGANPKTAGQYMAE
                     PNPVKEKWDDGDNVMCRLRYSGDADLTLWTKTQVTTSPVTGIDSVSATCGGTVTASAS
                     TVIEARGVCWSMYTNPSVSDNLTTNGSGPGSFTSELEGLTPGTPYYVRAYATIKNGQT
                     IYGNEVSFTTDGGGLYIGKEYGGGRIFYIDNTMQHGLIAAETDQSNGAPWGCPGVEVG
                     GTWSDIGYGQANTSAILAACGSAGIAARICDDLVLNGLSDWYLPSFYELNYLFLYNQQ
                     IGGFSATEYWTSSEWDENQAVTWDFSTGNNAIRPKDYSSAHVRAIRSF"
     gene            3893..4378
                     /locus_tag="PKZ74_10065"
     CDS             3893..4378
                     /locus_tag="PKZ74_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM93144.1"
                     /translation="MKASLRFFLFLITSLFLFPLMALSQDPLNIEIGRFLDEWHNDAS
                     RADMQAYFDKIDDEGIYIGTDETENWTKLAFYDWSKPYFDKGKAWSFQADERNIYLSE
                     DQKMAWFDEKLRSSSGPLRGSGVLIYRNGNWKILHYVLSLPVPNEKFNDVMKVIAEDM
                     R"
     gene            4438..5139
                     /locus_tag="PKZ74_10070"
     CDS             4438..5139
                     /locus_tag="PKZ74_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93145.1"
                     /translation="MLLTSMLSAEQIKTLALSLGADRCGIADAARFDDAPKGFQPRDI
                     YSKCHSVIVFLKRLPVEVLRSGNPVVYTHTAGFLYRELDRIGMELCYRLEKEGIHPVP
                     VPTDDPYMHWEPERMHGMGILSMRHSARNAGLGILGRNTLLINEEMGNIVYIGAVLVD
                     AILEADPLVTDFHCPPHCRLCIDACPVGAFDGVTVIQERCRKHSIASHPRGWSLYTCS
                     ACRAACPYAEGTGKG"
     gene            complement(5213..5956)
                     /locus_tag="PKZ74_10075"
     CDS             complement(5213..5956)
                     /locus_tag="PKZ74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM93146.1"
                     /translation="MIIKEIRAKTILSASKIYDYVINPYVGCEHGCSYCYARYIKKFT
                     GHYEPWGGFVDVKVNAPELLKKEILKKKKAMVWISGLCDPYQPPEAHYELTRQCLEVL
                     AFYDWPVSIQTRSALVLRDLDMMNKFSELQAGFSIPTADDSVRMIFEPHATPVGDRIS
                     ALEELHRHGITTYAMIAPVLPGAENLVSLLAGKVDHILVDRMNYRYAEGIYKKHGLES
                     KMTDDYFAETGMLISEQCRKAGIACRVVF"
     gene            complement(6007..>6820)
                     /locus_tag="PKZ74_10080"
     CDS             complement(6007..>6820)
                     /locus_tag="PKZ74_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM93147.1"
                     /translation="DYLDVYGIKLASGRFFDKSYATDKEACVINQCAVNHYRFADPFG
                     IRFIVPTGSNPEDVEVRPVIGVVNDFHFESLRTRIGPYIMHFKDERNNWGYVSLRLGP
                     ETGPSTLAEIEKVWASYTSNDPLQYFFMDKDLERLYKEERQNSKLAVMFTILGILVAS
                     LGLFGLTSYTVQQRTKEIGVRKTFGASVYNIWYMIAREILLLITISAVLAIPFAWWIA
                     GNWLQNYEYRISLQVFDFLAGFLIAIVIALMTISYRAIRTAQMNPVVALRYE"
BASE COUNT         1621 a         1758 c         1645 g         1796 t
ORIGIN      
        1 ccgccatctc gccttcccag cttttaaaaa gtgtaaactg aaaatgtcaa aaagtgtaaa
       61 ctaaatggtt attggagaca tatccgccaa aatccctcaa tatcattacg ggaaccggaa
      121 taagttacac ccggaacgac gggattaacg tcatttcgcc aacatccctg ggtgcataat
      181 gaggtagttg gctctgcctg ctatcgattt cgcctgatga tcagcttaac acgctcttgc
      241 gtgaccatgc cgccgtgttc tctcatcagc ctggcaaaat atactccttc gggcaactga
      301 cctgttccga tcaccgttct ggggctcaca agaagataat gcccaatttc caggcctgtg
      361 aagctatgca gtgtcaattc catttggccc cgaaaatcag ctattttaag ggtgaaatag
      421 gtggatgcag gattgggact gatagtaaaa tcaatggatt tgccggattc agatatccca
      481 actccctcca ggtcaatagt ctggaatatc gtatcggaga ttccgcaggc tccggcaaca
      541 aggcttacct cataggttcc aggtgatgca tagctatggg tcgggttcat gatatacgat
      601 gtgccgccgt cgccaaaatc ccattgaaat tcggtcgcat acaatgaatt gtttataaat
      661 gtgatctgta gctcttccga atccagatag ctgaatcctg caatggggtc atactccccg
      721 atatgccagg ttaacaggct gtcgtacacc acggtctttg cggcagccct gatagtggcc
      781 gcatccgttg ccgggagtgc tgcatcaaaa gtgatcgatt ccgggtcctt tctgaagaat
      841 gaggcataaa aactgcatgc ggcggcataa gtgcctgcaa cagagggatg actgccgtcg
      901 gcttgatact gttcgatgga gggatcatgc tgccggatat acttccagac agcgccgacg
      961 ggagatacga tcgcctggtt gctgtcggcc atcatgcagt atcttagatt caaaagactg
     1021 tccatgcctg catatgtgca taccggaggc caccaggaac aattcgactc atccccgttt
     1081 tttctccccc aggtcatgaa aaatgctgtt tcggcacagg gattttctgc attgatgatg
     1141 ctgtctaaaa tccgggcata cgggaacacc tcggtttcca cctggctgat gggaaatgac
     1201 gggagctggc tttgctcctg caagaccaca tagtcccagt taccttccgc gattttttca
     1261 agcgatacca ggtttgtgga atgcccctcc agcgtgtacc cgcctggcat attgctgtca
     1321 taaagcaagg tgtcgcctgc tgaaaccgct acgtcggcaa ccatttgtgg taaattgttt
     1381 gtgtatgtat agctgtttcc caggaacaat gcccttttgg taagggattg tgcgttggac
     1441 aagctgcata ttaacagtac aaaaatttca atgctgattc tcatgatttg atcaattcta
     1501 aaaatccttc cggttcagtt cttaatgggt gtgatccggt caatggccaa aattaattca
     1561 tttcccgctt ccgttccaac cgggatgatg ccaatgccga agccctgagt gtgactactg
     1621 agatttgcga gccgacagga tctccgtcac caatgcggcg ggacaggtgt tgtttgagca
     1681 gaggatcaaa gagggttccg tcattgaaag cgtggatatt tcggattttc ctgtcggtgt
     1741 atatttcgta aaaacgatat caaaggatca cgtttcggtc ttcaggttcc agaagagata
     1801 agcttatcaa caatcctggc taaaggtgag tatttggaga taatgcattt ctaaaaagaa
     1861 cggatcgctc tcacgtgggc cgatgaataa tctttaggcc gaatcgcatt attcccggtg
     1921 ctaaaatccc atgttactgc ctggttctca tcccattcac ttgacgtcca atactctgtt
     1981 gcagagaaac cgccaatttg ttgattgtac aagaacagat aattcaactc ataaaatgat
     2041 ggcaaatacc agtcgcttaa tccattcaga accaaatcat cgcaaattct ggctgcaatg
     2101 cccgccgatc cgcaggcggc caggatcgca ctggtattag cctgtccgta acctatatct
     2161 gaccacgttc cgccgacttc aacgccggga caaccccagg gggcgccatt gctttgatcc
     2221 gtttcagccg ctatcaggcc gtgttgcatg gtgttgtcga tgtaaaagat tctcccgccg
     2281 ccatattcct tgccaatata gagaccccca ccgtccgtag taaaagaaac ttcatttcca
     2341 tagatggtct gcccgttttt aatcgtagca taagccctta cataataggg tgttccgggt
     2401 gtaagacctt ccagctcgct ggtgaaactg cctggtccgg agccattggt ggtcagatta
     2461 tcactgaccg atggattggt atacatgctc cagcacacac cccgggcctc aatcacagtg
     2521 gaggccgatg cagttaccgt acctccgcag gttgccgaaa ccgagtcgat gccggtgaca
     2581 gggctggtcg taacctgcgt ttttgtccat aatgtgagat ccgcgtcacc ggaatacctc
     2641 agcctgcaca tgacattgtc accatcatcc cacttttcct tgacagggtt cggttcagcc
     2701 atgtactgac cggccgtttt gggattagcc ccggccgtgt ccgacaggta ttcaatagca
     2761 gcggttccaa ggttgcaatt gaagttggtc ctgacccgcc acgaaaatcc catataggtt
     2821 ccctttgcaa acgactcttc gatcaatggc caggtaccca gaaagctgaa gcaaaatcca
     2881 ccgtaaaaga acactgtatc tttgctgaag tcattatggc ttgtaataaa atcctttgaa
     2941 ataaagtaaa ccggtgaagg gtcaagctgg ctgtttacgt tcttcacatt aaagattgcg
     3001 atttttcctt ccttgatatc atccccgtat tttcttgttg tgccattgga aacagattcg
     3061 ccggtttgca ggtatgtttc cacaacatcc cattttgtgg gatgggccca tccatgcgaa
     3121 tagatacgga ttatcccgta gccgccgagt tcacagaacc tgtcaagcgt cgcatcacga
     3181 tctttataga cttttttcag ctcatatcct acccgctcca ggtcttcagt gtttttcggg
     3241 ataatcatat cagtcaattc cgtccgttcc caattggagg gatttaaaaa gatcatcttg
     3301 ctgttgttta caattgattt taattccccc ggcaacttat ccgatgtatg gcctcctgcg
     3361 ggcatgcttt tatcctctcc gtaaaagtct tccggattaa gcatgatccc tcccctcatc
     3421 ccgtttttgt aatcaatggc gataccctgg cttccgacaa ccgcagatac aacggctgta
     3481 tcgcccagaa agaattgctg aagctggtcc acagcctcca gcgaatccat aatatccagc
     3541 caaacataga agttgtcggt cccctggttt tgcagttcta gaatatgctc gtaataggcc
     3601 tggtcctcct cggtccagtt tgaattttct tcattcttgt tacgtgccgt aaggatggtc
     3661 aaaatgatca aaagataata aaccggattt ttcatgattt ggctaatttt tgaagaacac
     3721 gcaatttaag caattctgaa gtgattgtca aatatttttc aggtcctcgc ttcattctgc
     3781 ctggttttaa taatcctttt catttccgga ggcgatcaat tattgttagc agccaaaggg
     3841 gaaataccgg ttacctcgca gaggtttaac tatctttacc aaaacaaatc ccatgaaagc
     3901 ttctcttcgt ttcttcctgt ttttgatcac cagcctcttt ctatttcctt taatggctct
     3961 ttcgcaggat cccctcaata tcgaaatcgg ccggtttctc gacgaatggc acaacgacgc
     4021 ctcccgggcc gacatgcagg cctatttcga caagatcgac gatgaaggga tatatatagg
     4081 caccgatgaa acggaaaact ggaccaaact ggcgttttac gactggtcga agccttattt
     4141 cgacaaagga aaagcatggt cgttccaggc cgatgagcgc aatatttatc tttcagaaga
     4201 tcaaaagatg gcctggttcg acgaaaagct gcgttcctct tccggccctt tgcgcggttc
     4261 cggcgtgttg atctatagaa acgggaattg gaagatctta cattacgtcc tttccttacc
     4321 ggttccgaac gagaaattca atgatgtgat gaaggttatt gcggaggata tgcgatgatt
     4381 tcctgaaggc accaatgcat tcgtgctgtt ccggccgtat acctgaaatc cttatatttg
     4441 ctcctgactt ctatgttatc tgctgaacaa atcaaaaccc tcgccctgtc gctgggggcc
     4501 gacagatgcg gaatcgccga tgccgcccgt ttcgatgatg cgccaaaggg tttccaaccc
     4561 agggatattt acagcaaatg ccattcggtg atcgtcttcc tgaagcgcct tcccgtagag
     4621 gtgctccgga gcgggaatcc cgtggtgtac acccatacgg ccggttttct ctaccgtgag
     4681 ctcgaccgga tcggtatgga gctatgttac aggctggaaa aggaaggaat tcacccggtc
     4741 cctgtgccta ccgacgatcc ctacatgcac tgggaaccgg aacggatgca cggtatgggg
     4801 atcctgtcga tgcggcacag cgcccgcaac gccggcctcg gaatactggg aaggaataca
     4861 ctgctgatca acgaagagat gggcaatatc gtttacatcg gcgctgtcct tgtcgatgcc
     4921 atcctggagg ccgatccgct cgtcacagat tttcactgcc cgccccactg ccggctttgt
     4981 atcgacgcct gccccgtcgg cgccttcgac ggtgttaccg tgatccagga acgctgccgg
     5041 aagcattcca tcgccagcca cccgcgcggc tggagccttt atacctgctc agcctgcagg
     5101 gcggcctgtc cgtatgcgga gggaacgggg aagggctgat aaagtgtatc ctgttgttgt
     5161 aatggtagat gatttgatct ttcaggaatc attctgaaaa ttattctgaa ggtcaaaaaa
     5221 ccaccctgca tgctattcct gctttccggc attgctccga gatgagcatt cctgtttcag
     5281 cgaaataatc gtcggtcatt ttgctttcca gcccatgttt cttatagatc ccctcggcat
     5341 agcggtaatt catccggtcg acaaggatat ggtcgacttt tccggcgagc agggacacca
     5401 ggttttccgc cccgggcagt accggtgcga tcatcgcata ggtggtgatg ccgtgacggt
     5461 gcagctcctc cagtgctgaa atccgatctc cgaccggcgt agcgtgaggc tcaaagatca
     5521 ttctgaccga atcatcggcg gtggggatgg aaaagccggc ctgcaactca ctgaatttat
     5581 tcatcatatc caggtctctc agtaccagcg ccgaccgggt ctgtatcgat acgggccagt
     5641 cgtagaaggc cagcacctca aggcattgcc tcgtcagttc gtaatgtgct tcgggcggct
     5701 ggtaaggatc gcacaaaccg ctgatccaga ccatggcttt ctttttcttc agtatctctt
     5761 tcttgagcaa ttccggtgca ttgaccttta catctacaaa cccgccccac ggctcgtaat
     5821 gccccgtgaa cttcttgata taacgggcat agcagtaaga acatccgtgt tcgcatccga
     5881 catacggatt gatcacatag tcgtagatct ttgatgctga aaggattgtc tttgccctga
     5941 tttctttgat aatcatgggt taaaggtaat taattccggt cgttggtcgt ttttcccttt
     6001 tccctttcac tcgtacctta gcgctaccac cgggttcatc tgcgccgtcc tgatcgcacg
     6061 gtagctgatg gtcatcaggg cgataacgat ggcgatcagg aagccggcca ggaaatcgaa
     6121 cacctgcaag ctgatccggt attcgtagtt ctgcagccag ttgccggcga tccaccaggc
     6181 aaacgggatg gccagcacgg ccgagatcgt gatgagcaaa agtatctccc tggcgatcat
     6241 gtaccagatg ttgtacaccg aggccccgaa ggtctttctc acgccgatct ctttggtgcg
     6301 ctgctgtacg gtgtaggagg tgagcccgaa aagcccgagc gacgcgacca ggatgccgag
     6361 aatggtgaac atgacggcga gcttcgagtt ctgccgttct tccttataga gccgctccag
     6421 gtccttgtcc atgaagaagt actgtagggg atcgttagat gtgtatgagg cccatacctt
     6481 ctcaatctcc gccagcgtcg aaggaccggt ctcagggccc aggcggaggc tcacatagcc
     6541 ccagttgttc ctttcatctt taaaatgcat gatataaggc ccgatccggg ttcgcagcga
     6601 ttcgaagtga aaatcgttca ccacgccgat aaccggcctg acctcgacat cctccggatt
     6661 tgacccggtc gggacgatga atctgatgcc gaagggatcg gcgaaccggt aatggttcac
     6721 ggcgcactgg ttgatgacac aggcctcttt gtcggtggcg tagcttttat cgaagaaccg
     6781 gcccgacgcc agtttgatcc cgtaaacatc caggtagtcg
//
LOCUS       DAOPNH010000188         6806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12739_length_6806_cov_6.951711, whole genome shotgun sequence.
ACCESSION   DAOPNH010000188 DAOPNH010000000
VERSION     DAOPNH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12739_length_6806_cov_6.951711"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..366
                     /locus_tag="PKZ74_10085"
     tRNA            294..366
                     /locus_tag="PKZ74_10085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:327..329,aa:Gln,seq:ttg)
     gene            466..912
                     /gene="rimP"
                     /locus_tag="PKZ74_10090"
     CDS             466..912
                     /gene="rimP"
                     /locus_tag="PKZ74_10090"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPM93148.1"
                     /translation="MTIAEVTKIANEYLAGSDKFLVDVLVKPTNRVFVFIDGDQGVTI
                     DDCVKLSRHIESTFDRETEDFELNVSSCGADQPLRFSRQYAKNAGRMLQVKLDDDSIV
                     AGKLVSADESGIVIITNENKKKKVPSETFHLAFNKIKEAKVIISFK"
     gene            943..2175
                     /gene="nusA"
                     /locus_tag="PKZ74_10095"
     CDS             943..2175
                     /gene="nusA"
                     /locus_tag="PKZ74_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPM93149.1"
                     /translation="MDHINLVETFSEFKEFKNIDRETMMRILEDVFKHMLEKRFGTSD
                     NFKIIVNIDRGDLEIWRNRVIVEDGSVEDDNTQIALSDAIKIEPDFEVGEEVSEEMKL
                     IDFGRREILSIRQNLVSKIMEYEKDSIYRKYMEKVGDIITGEVYQVWKKEILVLDDEG
                     YELSLPKQEQIPSDFYRKGDTIRAVVARVEMKNNTPAIIISRTSPVFLERLFESEVPE
                     IYDGLITIKKIVRVPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INFTNNSSLFIQRALSPAKISSIHLDDVTKKAEVYMKPDQVSLAIGKGGYNIKLAGKL
                     TGYEIDVYREDIDSEDVDIQEFSDEIDQWIIDELKAIGCDTAKSVLELEVKELVKRTD
                     LEEETIREVIRILKAEFE"
     gene            2228..5188
                     /gene="infB"
                     /locus_tag="PKZ74_10100"
     CDS             2228..5188
                     /gene="infB"
                     /locus_tag="PKZ74_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPM93150.1"
                     /translation="MPDEVKPTRLNKAAAELNVGISTIVEFLHKKGIVIEENPNTKIT
                     PEIFALLVKEYQSDKAAKDEAKKIGLSFVKHESITLDEKGTKARAPEREPEQDELIIK
                     SSAVDDTITQKEKKPAAAKKPAEEEKPAPKPAPKEAKEPEPEKAPEIKPVVATPEPTP
                     GPVVEEKPVVAPAEAEIPTDSPIKVLGKIDLDSINLKTRPAKKTKEEKKAEAHRKFEK
                     TKAKPAEEEPTAEIIPEDKEEVVVAVTPEPEPVVVEPVKEEPPAEKKPHRDANFIPTR
                     VEKLDGPKIFGKIELPVEKKPEKKPVASSTDDKLQLKKKKRKRIKQEKYQEPVAQQES
                     KERQKPGAKKRQVFKPEPSEEEIQKQIKETLARLAPSGKSKTSKYRRQKRDSIREEIE
                     IDRQKAEEDKKILKVTEFVTVNEMANMMNVPVINIISTFMQLGMFVSINQRLNAESLA
                     LVAEEFGYKVEFVDVSVQEALELTQEEDKEEDLVDRPPIVTVMGHVDHGKTKLLDFIR
                     NTNVVAGEAGGITQHIGAYEVQLESGKKITFLDTPGHEAFTAMRARGALVTDVVIIVI
                     AADDDVMPQTVEAINHAQAGGVPLVFAINKVDKPTANPEKIKEQLAKMNILVEDWGGK
                     YQSQEISAKQGLNVGLLLDKVLLEAELLELKGNPNRLALGTVLESSLDKGRGYVAKVM
                     VQNGTLKVGDMVLAGAHFGRVKAMYNERNQRVDLAGPSTPVLLLGLDGAPQAGDKFNV
                     MKDEKEAKAIANKRLQLQREQGIRSQKHITLDEIGRRIAIGDFKELNLIVKGDVDGSV
                     EALSDSLLKLSTKEVQVNVIHKSVGQVTESDVLLASASNAIIVGFQVRPSLSARKLAE
                     KEEIEIRSYSIIYQAIEEIKAAIEGMLSPEIEEKITSNLEIREVFKISKVGTIAGCHV
                     LDGKITRNTRIRLIRNGIVVYTGRLGSLKRFKDDVKEVAAGFECGLNIDNFNDIKEGD
                     IIEGFEEVEIKRKL"
     gene            complement(5193..6482)
                     /locus_tag="PKZ74_10105"
     CDS             complement(5193..6482)
                     /locus_tag="PKZ74_10105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPM93151.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDVLTPGYDIIARFQGGPNAGHTIE
                     FNHKKFILHTIPSGIFYPEKTNLVGNGVILDPFIFLKEINNLADHGIDATANLVISKR
                     THLILPTHRLLDAAQETLKGAAKIGSTLKGIGPAYTDKIARNGLRAGDLLSPGFDNAY
                     QHMREAHLRYIKFLGYETDSFTIDKLSFDEYERQWLKAARFMAKLRLIESEYFIHKSL
                     AEGKKVLAEGAQGTMLDIDFGSYPFVTSSNTSVGGVCSGLGVSPAHIGEVIGVFKAYC
                     TRVGSGPFPTEQFGEEGARMRDTGHEYGSTTGRPRRCGWLDMVALNYSIMINGVTRLF
                     MMKTDVLDDFPVLKAGTGYRFDNSIADQVPFDMVNDAMEVVYEDFPGWNSPLQGLDNY
                     HKLPEALKKYVKFIEDRTKVPVIGLSYGPDRNQTLFF"
     gene            complement(6506..>6806)
                     /locus_tag="PKZ74_10110"
     CDS             complement(6506..>6806)
                     /locus_tag="PKZ74_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPM93152.1"
                     /translation="KYRVSRATLYNTIELLLDCSLVTKHQFGNNCAQFERSYKFKKHD
                     HFIDTTTGKVIEFSDPRIDDIRRFVEDTLKVRVSHHSLTFYGSSLDEHKEPALTH"
BASE COUNT         1989 a         1590 c         1750 g         1477 t
ORIGIN      
        1 cacctgcaat ttgtaggcag tggcgatgta ttccccgcaa tcccaccagc tggcggaagg
       61 ctccagggtc aacagataaa cgatgaaggc gatcaggaag gaagcccatc cgaaaagggt
      121 attccagcgg ataaaatctt tgtgtgctat catgctcctt tatttggagt tgcgaaaata
      181 gaaagaaaaa gcgaatcttt catcccgaat atgactgagc aaatatttct gaagcaaacc
      241 gaaatattaa ataaataatt tctatctttg cgcgcccaaa acaggggaca gtttgtccga
      301 tggtgtaact ggcaacacgt ctgattttgg ttcagaagac tccaggttcg agtcctggtc
      361 ggacaacagg attaatgaaa tagttaatct ttcgggaggg gactgcggtc cccttcttta
      421 ttgcatcaaa aaatgaaaaa tgaaaaatga aaaataacct gtcacatgac cattgctgaa
      481 gttacgaaaa tagcgaatga ataccttgcc ggcagcgaca aattcctggt cgacgtactg
      541 gtaaagccta ccaacagggt atttgtgttc atcgatgggg atcagggggt tactatcgac
      601 gattgcgtaa aactgagccg gcacatcgaa tcgacatttg accgggaaac tgaagatttt
      661 gagttgaatg tctcttcctg cggtgccgac cagccgctgc gtttttccag gcaatacgca
      721 aagaacgcgg gcaggatgct ccaggtcaaa ctggatgacg actccattgt cgccggcaaa
      781 cttgtatcgg ctgatgaatc cggtattgtc atcatcacca atgaaaataa aaaaaagaag
      841 gttccgtctg aaacctttca tctcgctttc aataaaataa aagaagcaaa ggtcattata
      901 tcattcaaat aattgtttaa tccaagctca aacaataaag ccatggacca tataaatctt
      961 gttgaaacct tttctgaatt taaagaattt aaaaacatcg accgtgaaac gatgatgcgt
     1021 atcctggaag atgttttcaa acatatgctg gaaaaaaggt tcggcacttc cgacaatttc
     1081 aagattatcg tcaatatcga ccggggtgac ctggaaatct ggcggaaccg ggtaattgtg
     1141 gaagacggat cggtagaaga tgacaatact cagattgcac tttccgacgc gatcaagatc
     1201 gaacccgact ttgaagtggg cgaggaagtg tcggaagaga tgaaactgat cgacttcggg
     1261 cgaagggaaa ttctctccat ccggcagaac ctggtgtcga aaatcatgga atatgagaaa
     1321 gacagcatct accggaaata catggaaaaa gtcggcgaca tcatcaccgg cgaagtttac
     1381 caggtctgga aaaaagagat cctggtcctg gacgatgaag ggtatgagct gagcctgccg
     1441 aaacaggaac agataccctc cgacttttac cggaaaggcg atacgatccg ggcagttgtt
     1501 gcacgggttg aaatgaagaa caataccccg gccatcataa tttcccgtac ctcgcccgta
     1561 ttcctggaac gtctttttga atcggaagtg cctgaaattt acgatggcct catcaccatt
     1621 aaaaaaattg tcagggttcc cggggaaagg gcaaaagtgg ctgtcgaatc gtacgacgac
     1681 cggatcgatc cggttggcgc atgcgtaggg atgaaaggat cacggatcca tggcatcgtc
     1741 agggaattga aaaatgagaa catcgatgtc atcaacttca ccaacaactc cagtcttttc
     1801 atccagcgcg ccctcagccc agccaagatc agctccatcc acctggatga tgtgacaaaa
     1861 aaggctgaag tctatatgaa acccgaccag gtctcgctgg ctatcggtaa aggaggatat
     1921 aacatcaaac tggcaggcaa gctgaccggg tacgagatcg atgtataccg ggaagacatt
     1981 gactcggaag atgttgatat acaagaattt tcagatgaga tcgaccagtg gatcatcgat
     2041 gaactgaaag ccatcggttg tgatacggcc aagagtgtac tcgaattgga ggtgaaggaa
     2101 cttgtcaagc gtaccgacct cgaagaggag acaatccgcg aggttattcg cattctgaag
     2161 gccgaattcg aataaaaata gtatttttgc accttttaaa aaaatagtgt aaactaaatc
     2221 tgtattcatg ccagatgagg taaagccgac caggctaaat aaagccgctg ccgaactgaa
     2281 tgtcgggatc tccactatcg tggaattcct gcataagaaa ggcatcgtga tcgaggaaaa
     2341 cccgaatacc aagatcacgc cggagatctt tgccctgctg gtcaaggaat atcagtcaga
     2401 caaggctgca aaggatgaag caaaaaagat aggcctctcc ttcgtcaaac atgaatcgat
     2461 aacactcgat gaaaaaggga ccaaggcccg cgcacccgaa agggaacccg aacaggatga
     2521 actgatcatc aagagttcgg cagtcgacga tacgatcacc cagaaagaga agaaaccggc
     2581 ggccgccaaa aaaccggctg aagaggaaaa accggctcca aagcccgctc ccaaagaagc
     2641 aaaagaaccg gagccggaaa aagcacctga aatcaaaccg gtcgtagcca ctcctgaacc
     2701 caccccggga ccggttgtgg aggagaagcc cgttgtggcc cctgccgagg ccgaaatccc
     2761 aaccgattcc cccatcaagg tactgggcaa aattgacctc gacagcatca acctgaaaac
     2821 caggccggca aagaaaacaa aagaagagaa aaaagcggag gcccatcgga aattcgaaaa
     2881 aacgaaagca aaacctgctg aagaagagcc cacggccgaa atcattccgg aagataaaga
     2941 agaggtcgtt gtggctgtaa ctccggaacc tgagccggtt gttgttgagc cggttaagga
     3001 agagcctccg gcagaaaaga agccgcatcg cgatgccaat tttattccca cgagggtaga
     3061 gaaactcgac gggcccaaga tttttggaaa aattgaactg ccagttgaga aaaagccgga
     3121 gaagaaaccg gtggcttcat ctaccgatga caagctccag ttaaagaaga agaaaagaaa
     3181 gaggatcaaa caggagaaat accaggagcc ggttgcacag caggaatcga aggagcggca
     3241 gaaaccgggc gcaaagaaaa gacaggtttt caaacccgaa ccctccgaag aagagatcca
     3301 gaagcagatc aaggaaaccc tggccaggct cgctccttcg ggtaaatcga aaacttcgaa
     3361 gtaccgccgg cagaaacgtg attccatccg ggaagagata gaaattgacc ggcagaaagc
     3421 tgaagaggac aaaaaaatac tcaaagtcac cgaatttgtt acggtgaacg aaatggccaa
     3481 catgatgaat gtgccggtca tcaatatcat ttccacattc atgcaattgg gcatgtttgt
     3541 ttcgatcaac cagcggctca acgccgagag cctggcgctg gtcgctgaag aattcggcta
     3601 caaagtggaa ttcgtcgatg tcagcgtgca ggaagccctg gaactgacac aggaggaaga
     3661 caaggaggaa gacctggtgg atcggccgcc gattgtcacg gttatgggac atgtcgacca
     3721 cggcaagacc aagctgctcg attttatccg gaacaccaac gtggtggccg gagaggccgg
     3781 tggtatcaca caacatatcg gtgcgtatga agttcagctg gagagcggga aaaagatcac
     3841 ctttcttgac accccgggtc acgaagcgtt tacggccatg cgtgcccgcg gcgccctggt
     3901 aaccgacgtt gttattatcg ttatagcagc cgatgacgac gtcatgccgc aaaccgttga
     3961 agctatcaac catgcgcagg ctggcggcgt accgctggta ttcgcgatta ataaagtgga
     4021 caaaccgacg gctaatcccg agaaaataaa agagcaactg gccaagatga atatcctggt
     4081 ggaagactgg ggcggcaaat accagtcgca ggaaatctca gccaaacagg gattgaatgt
     4141 cggtctgctg ctggataaag tattgctgga agcagaactt ctggaactga aaggcaatcc
     4201 gaaccgtctg gcactgggaa ctgtcctgga atcctcactt gataaaggcc gcggttatgt
     4261 ggcaaaagtg atggtacaga acggaacact gaaagtcggc gatatggttt tggccggcgc
     4321 ccatttcggc agggtaaaag ccatgtacaa cgaaagaaac caaagagtgg atttagcagg
     4381 gccatcaacc cctgtcctgc ttctgggtct ggacggcgca ccgcaggccg gcgacaaatt
     4441 caacgtcatg aaagacgaaa aagaagctaa agctattgcc aacaagcggc ttcagctcca
     4501 gcgcgaacag ggaatccgct cgcagaagca tattaccctc gacgaaattg gccgccgtat
     4561 agccatcggg gatttcaagg aactcaacct gatcgtcaaa ggcgatgtgg acggcagcgt
     4621 ggaagccctc tccgattcac tgctgaaact ctccaccaag gaagtccagg taaatgtcat
     4681 tcacaaatcg gtcggacagg tcaccgaatc ggatgtgctg ctggcctcgg catccaatgc
     4741 catcatcgtc gggttccagg tccgtccttc gctaagtgca cgcaaactgg ctgaaaaaga
     4801 agagatcgag atccggtcgt attcgatcat ttaccaggct atcgaagaga tcaaagccgc
     4861 tatcgaaggt atgctctctc ccgagatcga ggaaaagatc accagcaacc tggaaatacg
     4921 ggaagttttc aagatttcca aggtgggcac catcgcggga tgccacgtgc tcgacgggaa
     4981 aatcacccgg aatacacgca tccggctcat ccgcaatggc atcgtggtct atacgggacg
     5041 gctcggatct ctcaaacggt tcaaggatga tgttaaggaa gttgccgcag gttttgagtg
     5101 cggcctcaat atcgataatt tcaacgacat caaagaaggc gatatcatcg aagggttcga
     5161 agaggtcgaa attaaaagga agctttaatc cgctaaaaga acaacgtctg gttcctgtcg
     5221 gggccatacg acaggccgat caccggaact ttggtcctgt cttcaataaa tttcacgtat
     5281 ttttttaatg cttcgggcag tttatggtag ttatccaagc cctgaagcgg agagttccat
     5341 cccgggaaat cttcataaac aacttccata gcgtcattta ccatatcaaa gggcacctgg
     5401 tcggcaatgg aattatcgaa acggtatccg gtcccggctt tcagaactgg aaaatcatcc
     5461 aggacatcgg ttttcatcat gaacaggcgc gttactccgt taatcatgat tgagtagtta
     5521 agggccacca tatcgagcca accgcaacgt cgcggccttc ctgtcgtcga tccgtattcg
     5581 tggcctgtgt cgcgcattct ggctccttct tcgccgaatt gttcggttgg gaaagggccg
     5641 cttccgacgc gggtgcaata ggctttgaaa acgccgatca cctcgccgat atgggccggg
     5701 cttacgccca gtcccgagca aacgcctccg acggatgtat tggaagaggt tacaaaagga
     5761 taggaaccga aatcaatatc gagcatcgtt ccctgggcgc cttcagcaag aaccttcttt
     5821 ccttcagcca ggcttttatg gatgaagtat tcggattcga tgagtcgcag tttagccata
     5881 aatcgcgctg ctttcagcca ttgcctttcg tactcgtcaa aggaaagttt atctattgta
     5941 aaagaatcgg tttcatagcc caggaacttt atataccgga gatgcgcttc acgcatatgt
     6001 tgatatgcgt tatcgaagcc gggcgataaa aggtctccgg ccctgagccc gttacgggca
     6061 attttatcgg tgtaagcggg gccgatgcct ttcagagttg agcctatttt ggcggctcct
     6121 ttcaatgtct cctgggcggc atcgagaagc cggtgggtgg gcaatatcag atgtgtcctt
     6181 ttcgaaataa ccaaattggc cgtcgcatcg ataccatgat cggccaggtt gttgatctct
     6241 ttgaggaaaa tgaaaggatc gaggataacg ccgttgccaa ccagattcgt cttctccgga
     6301 taaaagatac ccgaagggat ggtatgcagg atgaattttt tatggttgaa ctcgatggtg
     6361 tggccggcat tcggaccgcc ctggaaccgc gcaatgatgt cgtatccggg tgtcaggaca
     6421 tccacaatct tccccttgcc ttcgtcaccc cactgaaggc caagaataac atctactttc
     6481 atttcaattg gtctggtgta taaatctaat gggtaagtgc gggttctttg tgttcatcca
     6541 ggctcgaccc gtaaaaagtg agcgaatggt gcgaaaccct gactttcagt gtatcttcca
     6601 caaaccggcg gatatcgtcg atccggggat cgctgaattc gatcactttt cctgtggttg
     6661 tatcgataaa atggtcgtgc ttcttgaact tgtacgaccg ttcgaactgt gcacagttgt
     6721 tgccgaactg gtgtttggtg accaggctgc agtcgagcag cagttcgatg gtattgtata
     6781 atgttgcgcg gctgacccgg tacttg
//
LOCUS       DAOPNH010000189         6766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_12844_length_6766_cov_6.970794, whole genome shotgun sequence.
ACCESSION   DAOPNH010000189 DAOPNH010000000
VERSION     DAOPNH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12844_length_6766_cov_6.970794"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..842)
                     /locus_tag="PKZ74_10115"
     CDS             complement(66..842)
                     /locus_tag="PKZ74_10115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM93153.1"
                     /translation="MVKISSVTVTLNEELNIERCLGSVRDVVDEMIVVDSFSTDRTEE
                     ICLENGAVFSKHPFAGYIEQKSYAISLASHDYILLIDADEALSDEMRDSIRKVKENWT
                     HDGYSFNRLNSFCGKWIKHSGWYPDRKVRLFDRRKNTVKGRNPHEEFVMNAGATLKHL
                     KGDLLHYTYLTVEDQVRQIDRFTAIQAREAFARGKKASWFLILFGPCYKFFRHYFLRL
                     GFLDGFYGYLICKNMAHSTFLKHAKLKALWIREKGIGKNN"
     gene            1085..6244
                     /locus_tag="PKZ74_10120"
     CDS             1085..6244
                     /locus_tag="PKZ74_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPM93154.1"
                     /translation="MKTKPFCFIMALLVAMAGCNKSTRTTDPVQFAEYVSGFTSGVIS
                     KADPVRIKLATGNLKFETGKDLPAGIISFEPSVAGTVSMYSEDLLIFKPSGEWPAGKT
                     IKAFLRLDKLMDVPPQFSTFNFQFSIITPSYSIYPGNLVSIGQGQNKMKKMEGKLVSA
                     DVMKLEEVDKLLKARADGKRYKVKWAEGSGRNEFEFVIDSLPRNEQAYQLELEWNGSP
                     LGIDHKGELAVTIPSIFDFIYLGYSIGQGEEQYLDIVLSDPVDPDQNLDGLVYLREGD
                     PVRLVADNNIIRLYPMERLERSRTLIVEASVRNDIRTSLKERVVQTVMFEELKPQAEF
                     IGQGVIMPDPAGLFLPVRAVSLRALDIVVYKIFENNIPYYLQENNFSDNHLYSLKQYG
                     RPVYAKTLMLDEDKSIDLHRWNNFSLDLSPLMEKDMGAIYRVRISFRKEYSVYHCDNK
                     AETDILEFILDGSVPDDQLEKYDQPGWYSDYEWPDEYDWDEQGNPCHNSYYIPERFAQ
                     KLIMASKIGVTAKSADTKQWIIHTTDLISGEPLTDVSVGLYNFQNQPVGSATTGPDGK
                     AEVTAESRPFFLKATKGKQTTWLRLDDGTSLSLSQFDISGAELQKGLKGMIYGERGIW
                     RPGDTLFLTFILDDELKRIPSDYPVLMEVYNSRGQIVYSQTRSDPAGQFYTFKVATLP
                     DAPTGNWRAVVKAGGAMFEKSLKVETVKPNRLKIKLDFDKEILNAFSGNPLGTLEVKW
                     LHGAVAPRVRTTVDMSFRKIKTGFRGYEKYAFDNPSGYFWWSEERNVLDQEMNDQGKG
                     SFSLELPKENNAPGMMEAVFLVRAFEKGGDFSTDVLTKTFSPFKRYVGIKVPNGGTYD
                     EMLETDEDQQIDIACVDWMGNPLTVRNLEVKVYRVDWRWWWSAGEDDLAYYVGGNDAN
                     LVYRGSADAVNGKGTFRFRIDYPDWGRYLILVKDNEGGHQAGKAVYIDWPSFRSRAGR
                     ANPAGATMLTFTADKERYEPGDKAVISFPASPGSRALVSVESGSRVLSTQWKMCEKQE
                     ETIEIPVTKGMAPNVYVYLSLIRPHSQTLNDVPLRMYGVIPLMIEDPETVLSPVITMP
                     GELRPEEKFSVTISEKYNRPMTFTVAVVDEGLLDLTRFRTPDPHGIFYAREALGVKTW
                     DMFDLVIGAFGARLERNLAIGGDEEAMLAKNKKAQRFVPVVRFAGPFTIGKGEKKEIH
                     FTMPNYVGSVRTMVIAGHDGAYGMVEKATPVKKPLMVLATVPRVIGTAEEFEVPVSVF
                     SMDEKIRDVTVTLESNDILTGLQNNVSLRFNQTGEQMAYFRLKATDREGIGKIKITAV
                     SGSETASHAVEFDIRNPNPVLTRTESFMISPGEKMEIPYEFFGSVGTNQGQVVFSGLP
                     DFDLQKHLEYLIRYPYGCLEQTVSGAFPQLFLSDLMELSEDRKTSTDRNIRAAIQRIS
                     QMTLADGSLTYWPGEIYYDRWATSYAGHFLVLASEKGYLVPPGVLEKWKEFQYKAAGN
                     YQPSPEDYYYWSNSNQAYRLYTLALAHKPNMSAMNRLRESGRLTPSATWILAAAYIYA
                     GKPEVATDLITGKDPNVLDEYGNAGYTYGSELRDRAFTLEVLTLLNRDQEAFKVMNEI
                     AKGLKFRYYSTQTTAFCLYAISRFAGAGATPGLSFEYRNENESVQTINTTKPVFSAPL
                     DPGRGLKAWCLRTTGRLCFCCPVVRKHHAFRSAVAGRGKGRFQLPGNSGHV"
     gene            6237..6635
                     /locus_tag="PKZ74_10125"
     CDS             6237..6635
                     /locus_tag="PKZ74_10125"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93155.1"
                     /translation="MYKDDAGKSLDVRSLKQGTDFVAEVTVSHSGVLFPYEELALTQI
                     FPSGWEIINTRVQDVNPGIKEDLFDYRDYRDDRVYTFFDLQLYEKKTFRVRLNAAYTG
                     RFYLPSVNAEAMYDDAIYAFTKGMWVEVVR"
BASE COUNT         1647 a         1648 c         1845 g         1626 t
ORIGIN      
        1 cagagcgcat gcttcacacg catgaggtca ctggttcgaa tccagtacat cccacttccc
       61 ctaatctaat tatttttccc tattcccttt tccctgatcc aaagcgcttt cagcttcgcg
      121 tgcttcaaaa acgtggaatg tgccatgttt ttgcagatga gataaccata gaaaccgtcg
      181 aggaatccga gccggagaaa ataatgccgg aagaacttgt aacaggggcc gaacaggatc
      241 aggaaccagc tcgctttctt ccccctggca aaagcttccc gggcctgtat ggccgtgaac
      301 cggtcgatct gccgcacctg gtcctccact gtaagatacg tgtaatgcaa caagtcgcct
      361 ttcagatgtt ttaaggtggc gccggcgttc atcacaaact cttcatgggg attccggccc
      421 ttgaccgtat tcttccgccg gtcgaacaac cttaccttgc gatccggata ccagccgctg
      481 tgctttatcc atttcccgca aaagctgttc agccggttaa agctgtaccc gtcgtgcgtc
      541 cagttctctt tcaccttccg gatagagtcc cgcatctcat cgcttaacgc ttcgtctgca
      601 tcgatcaaaa ggatgtaatc atggcttgcc agcgaaatgg catagctctt ctgctcaata
      661 tatccggcga aaggatgctt gctgaaaaca gctccgttct ccaggcaaat ctcctccgtc
      721 cggtcggtcg aaaaggaatc gacaacaatc atctcatcca ccacatcacg gaccgatccc
      781 aggcaccgtt cgatgttcag ctcctcgttc agcgtaacgg ttacggaaga aatttttacc
      841 ataatgcaaa atttttgcgg agggccaaag ttattcataa agatgcaatt tttattcttt
      901 gcctttgatt tattcgtacc ttggagcttt gttgtaagaa atgttatgaa tagtgctttg
      961 cggcattatt gccactaaaa caccaagaca ccaaagatca ccaaattgat ttttttggtg
     1021 ttatttagtg ttttggagcc ttggtggcat ataaagccat cttaattaaa atcaggctaa
     1081 tgttatgaaa accaaacctt tttgttttat tatggctttg ctggtcgcga tggccggttg
     1141 caataaatcg acaaggacca ccgatccggt tcagttcgcg gaatacgttt cgggatttac
     1201 ttccggggtg atctccaagg ctgacccggt caggattaaa cttgcgaccg gaaatttgaa
     1261 atttgaaacc gggaaagatc taccggcggg tattatctcg ttcgagcctt cagtcgctgg
     1321 gaccgtatca atgtattctg aagatttgct gattttcaag ccttccggcg aatggccggc
     1381 gggtaaaacc attaaagcct tcctgagatt ggataaactg atggatgtac ccccccaatt
     1441 ttcgactttc aactttcaat tttcaatcat taccccatct tacagcatct acccggggaa
     1501 cctggtctcg atcggccagg gacagaacaa aatgaaaaag atggaaggca aactggtatc
     1561 ggccgatgtc atgaagctgg aagaggttga caaactgctg aaagccagag ctgacggcaa
     1621 acggtataaa gtgaagtggg ccgagggatc gggccgcaat gagttcgaat ttgtgatcga
     1681 cagtttgccc cggaatgaac aagcctatca gctggaactg gaatggaacg gatccccgct
     1741 ggggatcgat cataaaggag agcttgcggt aaccattcca tcgatcttcg atttcattta
     1801 cctcggatat tccatcggcc agggtgaaga gcagtatctc gacatcgtcc tttcggaccc
     1861 ggtcgacccg gaccagaacc tcgacgggct ggtttacctg cgggaaggtg atccggtaag
     1921 gctggtggcc gataataaca ttatccgcct ctatccgatg gagcggctgg aaaggagccg
     1981 cacgctcatc gtcgaagcct cggtcaggaa cgatatccgg acatcattga aagaaagagt
     2041 cgtgcagacg gtgatgtttg aggagttaaa gccacaggcc gaattcatcg gccagggggt
     2101 gatcatgcct gatcccgccg gcctcttcct cccggttcgc gccgtcagcc tccgcgccct
     2161 cgatatcgtc gtttataaga tcttcgaaaa caatatccct tattatctcc aggaaaataa
     2221 cttttcggat aaccatttat acagccttaa acagtatggc aggccggttt atgccaaaac
     2281 gctgatgctg gatgaagaca aatccatcga cctgcaccgg tggaataatt tttccctcga
     2341 tctttcgccg ctgatggaga aagacatggg cgcgatctac cgtgtcagga tctccttcag
     2401 gaaagaatat tccgtatatc attgtgataa taaagccgaa acggatatct tggaattcat
     2461 ccttgacgga tccgttcccg atgaccagct ggaaaagtat gaccagccgg gctggtattc
     2521 cgattacgag tggcccgacg aatatgattg ggacgagcag ggaaatccgt gccacaactc
     2581 ctattacatc cccgaaaggt tcgcgcagaa gctgatcatg gcttcaaaga tcggcgtgac
     2641 ggctaaatcg gccgatacga aacaatggat cattcatacg acggacctga tatcgggtga
     2701 gcctttgacg gatgtatcgg tcgggttgta taatttccag aatcagcccg taggatcggc
     2761 cactaccggc ccggatggga aggcggaggt taccgccgaa agccggccgt tttttctcaa
     2821 agctaccaaa ggcaaacaaa ccacctggct ccggctcgac gacggcacct cgctgtcgct
     2881 cagccagttc gatatcagcg gggcagagct tcagaaaggg ctcaaaggga tgatatacgg
     2941 tgaaagaggt atctggcggc ctggcgatac cctctttctg accttcatcc tggatgatga
     3001 attaaagcgg atcccgtcag attatcccgt tctgatggag gtgtataatt ctcgcggcca
     3061 gatcgtttac agccagacgc ggtcggatcc tgccggccag ttctacactt ttaaagttgc
     3121 aacccttccg gatgccccta ccggcaactg gcgcgctgtt gtgaaagccg gcggtgcgat
     3181 gttcgagaaa agcctcaagg tggagacggt caaacctaac aggctgaaga tcaagctgga
     3241 tttcgacaaa gagatattga acgctttcag cggcaacccg ttgggaacgc tggaggtcaa
     3301 atggctgcac ggcgctgtgg ctccaagggt aaggacaacg gtcgatatga gtttccggaa
     3361 aatcaaaacc ggtttccggg gatatgaaaa atatgctttt gataatcctt ccggctattt
     3421 ctggtggtcg gaggagagga atgtgctcga ccaggagatg aatgaccagg gaaagggttc
     3481 tttcagcctc gaattgccta aagagaacaa tgcgccgggg atgatggaag ccgttttcct
     3541 tgtccgggct tttgaaaaag gtggggattt cagtacggat gttcttacca aaactttttc
     3601 tccgtttaaa aggtatgtcg ggattaaggt tccgaacgga ggaacgtatg atgagatgct
     3661 ggagacggat gaggatcagc agattgacat tgcctgtgtc gactggatgg gcaatcctct
     3721 gaccgtcagg aacctggagg tcaaagtgta ccgggttgac tggcggtggt ggtggagtgc
     3781 cggggaggat gacctggcct attatgtggg tggaaacgat gccaatctgg tttacagggg
     3841 gagtgcggat gccgtaaatg gcaaagggac cttccgtttc cggatcgatt atcccgactg
     3901 gggccgctac ctgatcctgg tgaaagacaa tgaagggggc caccaggcgg ggaaagccgt
     3961 gtatatcgat tggccctctt tcagaagcag ggcaggacgg gccaacccgg cgggcgcaac
     4021 gatgctgacg tttaccgccg ataaggagag gtatgaaccg ggcgataagg ctgttatcag
     4081 cttcccggcg tcgcccggca gccgggccct tgtcagtgtc gaatcgggga gccgtgtgct
     4141 ttccacgcaa tggaagatgt gcgaaaaaca ggaggaaacc atcgagatcc ccgtcaccaa
     4201 agggatggcg ccgaacgttt atgtctacct gagcctgatc cgtccacaca gccagacgct
     4261 gaatgatgtc ccgctgagga tgtatggcgt gatacccctg atgatcgagg acccggaaac
     4321 agtgctgtcg cccgtcataa cgatgccggg agaactccgg cccgaagaga aattctccgt
     4381 tacgatcagc gaaaagtaca accggccgat gacctttacg gttgccgttg tcgatgaagg
     4441 gctgcttgac ctgacccgtt tccggacgcc cgacccgcac gggattttct atgcccgtga
     4501 ggcgctgggg gttaaaacct gggatatgtt cgacctggtc attggggcat ttggcgcccg
     4561 gttggagcgg aacctggcca ttggcgggga cgaagaagcc atgctggcta aaaataagaa
     4621 ggcacagcgt ttcgtgcctg tggtgaggtt tgccggtcct tttaccattg ggaaagggga
     4681 gaagaaagag atccatttca cgatgccgaa ttatgtgggt tctgtccgaa ccatggtcat
     4741 cgcaggtcat gatggcgctt acggtatggt agagaaagcc actcccgtca aaaagcccct
     4801 gatggtgctg gctacggtcc cgagggtcat cggaactgcg gaggaatttg aggtgcctgt
     4861 ttccgttttt tcgatggatg aaaaaatcag ggatgttacg gtgacgctgg aatccaatga
     4921 tattctgacc ggattgcaaa acaatgtcag cctgaggttt aaccagacag gcgaacagat
     4981 ggcctatttc cggttaaaag cgactgatcg cgaagggatt ggaaaaataa aaataaccgc
     5041 cgtttccggt tccgaaacgg cctctcatgc ggtcgaattc gatatccgga atccaaaccc
     5101 cgtgctgaca cgaacggaat cctttatgat cagtccgggc gagaagatgg agatacctta
     5161 tgagtttttc ggttcggtcg ggacgaatca ggggcaggtt gttttctccg ggctccctga
     5221 tttcgatctg cagaaacacc tggaatacct gatccggtat ccgtacggct gtcttgaaca
     5281 aaccgtctcg ggcgcttttc cgcagctttt cctgtccgat ctgatggaat tgtcggagga
     5341 tcggaaaaca tcgacggaca ggaatatccg ggctgccatc cagcggattt cgcaaatgac
     5401 cctggccgac ggcagcttga cctattggcc gggtgagatt tactatgacc gatgggcgac
     5461 atcttatgcg ggacattttc tggtcctggc ttcggagaag ggatacctgg tgccgccggg
     5521 tgtgctggag aagtggaaag agttccagta caaagccgcg ggtaattacc agccatcgcc
     5581 tgaggattat tattactgga gcaacagcaa ccaggcttat cgcctgtaca ccctggccct
     5641 ggcccataag ccgaacatga gcgcgatgaa ccggctgcgt gaatcgggtc ggcttacacc
     5701 ttcggcaacc tggatcctcg ccgcagccta catctatgcc ggtaagcctg aggtggccac
     5761 ggatctgatc acgggtaaag acccaaatgt tttggatgag tacggaaatg ccggatacac
     5821 ttatggctcc gaactgcgcg acagggcctt cacactggag gtgctgacgt tgctcaaccg
     5881 tgaccaggag gcattcaaag tgatgaacga gatcgccaaa gggctgaaat tcagatatta
     5941 cagtacccag acgaccgctt tctgccttta cgccatttcc cgttttgccg gcgccggcgc
     6001 caccccggga ttatcttttg aataccggaa tgaaaacgaa tcggtacaaa ctattaatac
     6061 tacaaagccg gtcttttcgg ctccgctgga tccgggccgg ggattgaaag cgtggtgttt
     6121 acgaacaacc gggcgcctgt gcttctgttg cccggttgtt cgtaaacacc acgctttccg
     6181 gtcagccgtt gcagggagag gaaaaggaag gttccagtta cctgggaatt caggtcatgt
     6241 ataaagacga tgccggtaaa tctctggatg tgaggtcgct gaaacagggg accgattttg
     6301 tggctgaagt cacggtcagt cactcggggg ttttgtttcc ctatgaggaa cttgctttga
     6361 cccagatatt tccgtcggga tgggagatca tcaacacccg ggtccaggat gtgaatccgg
     6421 gtattaaaga agatcttttt gattatcgcg attaccgcga cgacagggtt tacacctttt
     6481 tcgacctgca gttgtatgaa aagaagacct tccgggtcag gctgaatgcg gcttataccg
     6541 gccggtttta cctgccctcg gtaaatgccg aagcgatgta tgatgatgcg atttatgcct
     6601 ttacaaaagg tatgtgggtt gaagtggtcc gttagaaaag cggttatttc agtggccggg
     6661 gttcttatcc tgatcatttt atggataatt ccctggtctc ccttgttcga tgacccattt
     6721 gcaacggtgg tttacgaccg gaatggccgg ttgcttgcgg ccagga
//
LOCUS       DAOPNH010000190         6690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13050_length_6690_cov_5.474454, whole genome shotgun sequence.
ACCESSION   DAOPNH010000190 DAOPNH010000000
VERSION     DAOPNH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13050_length_6690_cov_5.474454"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKZ74_10130"
     CDS             complement(<1..1212)
                     /locus_tag="PKZ74_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93156.1"
                     /translation="MIKSDALSFKKLLPVIVALAVFSLIIIIYFYPVLEGKKLSASDM
                     VHFKGMSKEVVDYRDKTGEEALWTNGMFGGMPAYQISVHYTQNLMTYIHRLLRLSFHL
                     PVGMIIIYFIGFYILMRVLKINPWLSITGAIAFAFSSYFFIILEAGHTSKAYAIGYMA
                     PVFAGIILSYRGRWIAGGLTTAFFLALLIVSGHPQITYYLLIAMLIYGLVELIRHYRE
                     KKLPDFLKATGVLVLAALLAVLTHTASLWNVYEYSKYSIRGKTELTSEQENRTSGLDK
                     DYATAWSYGISETMTLLIPNFHGGSSHGELSKNSETYQVLTQNRVPNADQIIKALPLY
                     WGDQPFTSGPVYVGAIIVFLFVFSLIYLKGPLKWWGIAVTVLSIMLAWGKNFMPLTDF
                     FLDYVPLYNKFR"
     gene            complement(1330..3573)
                     /locus_tag="PKZ74_10135"
     CDS             complement(1330..3573)
                     /locus_tag="PKZ74_10135"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPM93157.1"
                     /translation="MKKIIFPMVFFSFLIQTVNFAQSTIIIQPGPEGKDAQLWSITPD
                     LNIGNTPKFLASGWTHGGEPGINRALIDFDLSEIPENAVITSAKLNLYFLCLEPNFMG
                     HTGQNSATLSLVTEPWDEATVTWNTQPATTPENQVYLPQSAAPYMDYTDIDITGLIQS
                     LLTSPEEYHGIMMRLQNENPYRCILFASGDYSDTISMRPRLEITYTSESNTIVIQPGQ
                     EGKDALVWSAYPLYHFGNSPKFTCMAWSHYKEICTTRSMVEFDLSVIPANATITDARL
                     SLYFVSQENLIEGHTGENESVLRLITEPWEEDDVTWANQPETTTDFQVNLPASTDPYQ
                     DFTNIDVTNLISKQFEQPGQYHGLLLRLADESIYKCLLFASGDYAESAELHPKLEVSY
                     TVSTVTIEIQPGEEGKDAQINTILPGNNYGNSPIFKAAAWTHSGEPGTNRALVEYDLS
                     VLPENAVIEDARLNLYFANKEPTFYGHTGENDAWLQLITEPWEEMEVTWNNQPATTSQ
                     YQVYIPQSVEEFQDYTNIDVTEVITRLYNEPETYHGLLFRLIHEEYYRCLLFASSDYT
                     DTVENRPKLVITYSICQDPIAVYTFEKEDFSVYFHDQSLHGESLYWDFGDGYYSDLNE
                     PWHIYDNPGTYEVCLTVWNECGVDSVCHMINIEQNAEPSDTYDNTISIYPNPAVHYFN
                     VTSTIEEEVSVLLLSPNGEAVVKRIDNIDPDNGITIETSKLNPGIYFLKISGPSGHSI
                     KKIMVNH"
     gene            complement(3701..3850)
                     /gene="rpmH"
                     /locus_tag="PKZ74_10140"
     CDS             complement(3701..3850)
                     /gene="rpmH"
                     /locus_tag="PKZ74_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPM93158.1"
                     /translation="MKRTFQPSVRKRKNKHGFRERMSTKNGRKVLSNRRATGRKKLTV
                     SDEKR"
     gene            complement(3925..4653)
                     /locus_tag="PKZ74_10145"
     CDS             complement(3925..4653)
                     /locus_tag="PKZ74_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM93159.1"
                     /translation="MSKRIPKELFKDDHFLGRPADADDRIVHRRIKLVREFPGFTGKD
                     KTLLDIGCGNGASVIALHNDFRSCTGIDVEFKPDDGENYRFYVSDITKVRPEHQFDRI
                     ISFEVIEHLQEENLENYYKALKPGGIIAVSVPNKWWIFETHGANIPGLNKIPWNRVPL
                     FSWLPRPVHEKFANARIYTKCRIRKLLQKHGFHVVEIYYVTAPMDVLPEGGLKRFVIR
                     YLFNTDTTRLPFKATSIMVFAIKK"
     gene            complement(4671..6140)
                     /locus_tag="PKZ74_10150"
     CDS             complement(4671..6140)
                     /locus_tag="PKZ74_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPM93160.1"
                     /translation="MSEEIKKLEPKALWENFYRLTRVPRPSKHEEKIQQFMLEFGDEA
                     GLETFRDKVGNIIIRKPATPGMEDRKGIILQAHLDMVPQKNTATQHDFEKDPITAVID
                     GEWVKAKGTTLGSDNGIGAAAIMTILASKDMVHGPIEALFTCDEETGMTGANGLKPGL
                     LKGDILLNLDSEDEGELYIGCAGGVDVTADFSYKEVEVPPFVAPYRISLTGLRGGHSG
                     LDINLGRGNANKILNQLLMQAGEKFGVRMAQVEGGNLRNAIPREAFATVVVPESRKED
                     FKNFVAAFEENAKTMFGQADPGLMITVNSTKMPTHLMEEEVQNKLYKALDQAPNGMIS
                     MINDMPEVVETSTNLAIVKSKEGKVFVACLLRSSVDENKMKLAEQMSAIFTQAGAKTE
                     VSGEYPGWKPNIHSPILETMKEVYRVHFGKIPAVKVIHAGLECGILGAQYPHWDMISF
                     GPTIRGPHSPDEKVHIASVGLFWDYLVKTLEHCPVKNPA"
     gene            complement(6365..>6690)
                     /locus_tag="PKZ74_10155"
     CDS             complement(6365..>6690)
                     /locus_tag="PKZ74_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM93161.1"
                     /translation="FSLVPASMWPSVPKIVEDRYLGSAYALVFWIQNIGLWFIPWLIG
                     IVLKAVNPGVAEKIQAGDLTATYNYTVPMLIFSGLGMAAIFLGYLLKREDKRKQYGLE
                     LPNKR"
BASE COUNT         1621 a         1613 c         1584 g         1872 t
ORIGIN      
        1 cctgaactta ttatacaagg gaacataatc gaggaagaaa tcggtcaggg gcatgaaatt
       61 cttaccccag gccagcatga tagaaagcac ggtcacggca ataccccacc atttcaatgg
      121 gcctttcagg taaatcagtg aaaagacgaa gaggaacacg atgatagcgc ctacataaac
      181 gggaccggag gtgaatggct gatctcccca gtagagcgga agtgctttaa tgatctggtc
      241 ggcgttgggt acgcggtttt gagtaaggac ctgatatgtt tccgaatttt tggatagttc
      301 gccgtgtgat gagcccccgt ggaaattcgg gatcagcagc gtcatcgttt cggagatacc
      361 gtagctccat gcggttgcat agtctttgtc cagcccgctg gtcctgtttt cctgttctga
      421 tgtcagctcg gttttccccc ggattgaata tttgctgtat tcatagacat tccagagact
      481 tgccgtatgt gtaagaaccg ccagcaatgc tgccaggacc agcacgccgg tagctttcag
      541 gaaatccgga agtttctttt ccctgtaatg acggatcagt tccaccaaac cataaataag
      601 catcgcgatc aagaggtaat aggtgatctg gggatggccg ctgacaataa gaagggcaag
      661 aaaaaatgca gtcgtgaggc cgcccgcaat ccatctgccc ctgtatgata ggatgattcc
      721 cgcaaacacc ggtgccatat atccgatggc ataagccttt gacgtatgac ctgcttcaag
      781 gataataaaa aagtaggaag aaaaagcgaa tgcgatggct ccggtaatgc tgagccaggg
      841 attgatcttc aataccctca tcagaatata aaatcctatg aaatagatga taatcatacc
      901 taccggcaaa tggaaactta accttagcag gcgatggata taagtcatca aattttgagt
      961 gtagtgaacg gagatctgat aagcgggcat cccaccgaac attccatttg tccatagtgc
     1021 ctcttcaccg gtcttatcgc ggtaatcaac aacttccttc gacatccctt taaaatggac
     1081 catatcgctg gccgacagtt ttttgccttc caggacaggg taaaaataaa taatgatgat
     1141 gagggaaaaa acggccaggg caacaatgac cgggagtaac ttcttgaaac tcaaagcatc
     1201 tgacttgatc atctggatac aggtattgat taatacacgt attaacctcg cgaaattaaa
     1261 gaaattcggt taatcttatt ttaaattaaa tgtacctgtt tgtaagttac aggatatcaa
     1321 ttcttaatat cagtgattta ccatgatctt cttaatagaa tgacctgaag gccctgatat
     1381 tttcagaaag taaattcccg gattcagttt gctcgtttca attgttatcc cattgtcagg
     1441 atcgatgtta tcaatgcgtt taacaacagc ttccccgttt ggactcagca ataagacact
     1501 tacttcttcc tcaatggttg atgtaacgtt aaagtaatgg accgcaggat tagggtatat
     1561 ggatatggta ttatcatatg tatctgatgg ttctgcattt tgttcaatgt tgatcatatg
     1621 gcacacggag tccaccccgc attcattcca taccgtcagg catacctcgt atgttcccgg
     1681 attatcatag atatgccagg gttcattaag atccgaataa taaccatcgc cgaaatccca
     1741 gtacaaagat tcaccgtgaa gcgactggtc atggaaatat actgaaaaat cttccttttc
     1801 aaatgtataa actgcaatcg gatcctggca gatggaatag gtgatcacca gtttaggcct
     1861 gttctcaact gtgtcggtat aatcgctgga tgcaaaaagc aggcagcggt aatattcttc
     1921 atggatcaac ctgaacaaca gcccgtggta agtttcaggc tcgttgtaca acctggtgat
     1981 gacttcagtt acatcaatat tggtgtagtc ctggaattcc tcaacacttt gaggaatgta
     2041 aacctggtat tggctggttg ttgccggttg attattccag gtaacttcca tctcttccca
     2101 gggctctgtg atcagttgca gccaggcgtc gttctcaccc gtatggccgt aaaatgtagg
     2161 ttctttgttc gcaaaataga gattgagtct tgcgtcttca atcaccgcat tttccggcaa
     2221 tacggaaagg tcgtattcaa ccagcgccct gtttgtgcct ggttctccgg aatgcgtcca
     2281 ggcggctgct ttgaaaatgg gtgaatttcc ataattatta ccgggaagaa tggtgttgat
     2341 ctgagcgtct ttcccttcct ctcccggctg gatctcaatg gtgacggttg atacagtgta
     2401 tgatacttcg agtttcgggt gtagttccgc cgattctgca taatcacctg atgcgaacaa
     2461 aagacatttg tatatgcttt cgtctgccag gcgcagcagc aatccgtgat actgtccggg
     2521 ttgttcgaat tgcttggaaa tcaggtttgt aacgtcaata tttgtaaaat cctggtaagg
     2581 atcggtactg gccggcaggt tgacctggaa atccgttgtg gtttccggtt ggttggccca
     2641 ggttacatca tcctcttccc agggttccgt aattaaccgg agaacacttt cgttttcacc
     2701 ggtatgccct tcgatcaggt tctcctggct gacaaagtac agactgaggc gtgcatcggt
     2761 gatggtggca tttgccggga tgactgataa atcaaattct accatcgaac gggttgtgca
     2821 aatttctttg tagtgcgacc aggccatgca ggtgaatttg gggctgtttc caaaatgata
     2881 taaggggtaa gcgctccaga ccagtgcgtc tttcccttcc tgtccgggtt gaatgacaat
     2941 agtgtttgat tcgctggtat aggtgatttc cagcctggga cgcatagata tcgtgtcaga
     3001 ataatcaccc gaagcaaaaa gtatgcaccg gtaaggattt tcattttgca accgcatcat
     3061 gatcccgtgg tattcttcag gtgatgtcag cagggactgt atcagtccag tgatgtcaat
     3121 atccgtataa tccatataag gagctgcgct ctggggcaga taaacctggt tttccggggt
     3181 ggtggcgggc tgtgtgttcc aggtgaccgt tgcctcatcc cagggttcag taaccaggct
     3241 gagggttgcg ctgttctggc ctgtatgccc cataaaattg ggctcaaggc atagaaaata
     3301 gaggttcagc tttgcactgg taataacggc gttttccggt atttcagaca ggtcgaaatc
     3361 aatcagggcc ctgttgatgc ccggttcacc gccgtgcgtc cagcctgagg caaggaattt
     3421 gggagtattg ccaatattga gatctggcgt gatgctccac aactgagcat ctttcccttc
     3481 cgggccgggc tggataatga ttgtgctttg tgcgaaattg acggtttgga ttaagaacga
     3541 aaagaaaacc atcggaaaaa taatcttttt catggtgtct ggtttttcag gtaaggctat
     3601 aaatatagac aggaatttta tacgatgagt tgcactggca aaaaaaaaga agataaccat
     3661 gattatcttc ttttcgggtt cctgatattt taaaaggatt ctatctcttt tcgtcggata
     3721 cggtcagttt ttttctgccg gtagctcttc tgtttgaaag aaccttgcgg ccgttcttgg
     3781 tcgacatcct ttccctgaaa ccgtgtttgt tcttgcgttt ccttaccgat ggctgaaatg
     3841 ttcttttcat cacttctcaa ttttggaggt gcaaaagtac aatttaattt tgatctacca
     3901 aataaaatcc tcccgacttt gcaattattt tttaatggcg aaaaccataa tggaggttgc
     3961 tttgaaaggc agccttgttg tatcagtatt aaagagatac ctgatgacga atcttttcag
     4021 ccctccttca ggtaaaacat ccataggagc agtcacgtaa tatatttcca ccacatggaa
     4081 cccgtgtttt tgcaacagct tcctgatcct gcactttgtg taaatcctgg catttgcaaa
     4141 cttctcatgt acaggccggg gcagccagga gaacaaaggg accctgttcc aggggatttt
     4201 attaagtccg ggtatattcg cgccatgcgt ttcgaatatc caccatttgt tggggaccga
     4261 aaccgcgatg atcccgcccg gctttagagc tttgtaatag ttctccaggt tttcctcctg
     4321 aagatgttca ataacctcaa aactgataat acggtcgaat tgatgttccg gtcttacttt
     4381 tgtgatatcg gacacataga accggtagtt ttctccgtcg tccggcttga attccacgtc
     4441 aataccggta caactcctga aatcgttatg cagcgcgata acagaggctc cgtttccaca
     4501 accgatatcc aggagcgttt tatccttacc cgtaaatccc gggaactccc gcaccagttt
     4561 tatacgtcgg tgaacaatcc tgtcatcggc atccgccggg cgccccagaa aatgatcatc
     4621 cttgaaaagc tcttttggga tccttttgct catctcagcg ataagatatg ctatgcaggg
     4681 tttttcaccg ggcagtgctc cagcgtcttc accaggtaat cccagaacag gccgacggag
     4741 gcgatatgca ccttttcgtc gggggagtgc gggccgcgga tcgtgggtcc gaatgagatc
     4801 atatcccagt gcggatattg cgcgcccagg ataccgcatt cgagccctgc gtggatcacc
     4861 ttgacggccg gaatcttccc gaaatggacc cggtaaacct ctttcatagt ctccaggata
     4921 ggggagtgga tattgggttt ccaacccggg tattcacccg aaacctccgt tttggctcct
     4981 gcctgggtga agatggccga catctgttcc gccagcttca tcttgttctc atccaccgaa
     5041 ctgcgcagca ggcaggcgac aaatactttg ccctccttcg atttgacgat ggccaggtta
     5101 gtggaggttt ccaccacctc gggcatgtcg ttgatcatcg agatcatgcc gttgggcgcc
     5161 tggtcgagcg ctttgtacag tttgttctgc acctcttctt ccatcaggtg cgtgggcatt
     5221 ttggtggaat tgaccgtgat catcaggccc gggtcggcct gaccgaacat ggtcttggca
     5281 ttctcttcaa atgcagccac aaagtttttg aaatcttctt tgcgggattc gggtaccact
     5341 accgtcgcaa aagcctcacg cgggatggca ttgcgcaaat tgcccccttc cacctgtgcc
     5401 atgcgcaccc cgaatttttc accggcctgc atcagcaact ggttcaggat cttgttggca
     5461 ttgccccgtc ccaggttgat atccagtccc gagtgcccgc cccgcagccc cgtcaggctg
     5521 atacggtacg gcgccacgaa cggcggaact tctacctctt tgtagctgaa gtcggccgta
     5581 acatccacgc cgccagcgca accgatatac aactcgccct cgtcttccga atcgaggttc
     5641 agcaggatat cgcctttcag caaccccggt ttcaggccat tggctccggt catcccggtc
     5701 tcttcgtcgc aggtgaacag cgcttcgatc gggccgtgta ccatgtcttt cgacgccagg
     5761 atcgtcatga tcgccgctgc gcctattccg ttgtcgcttc ccagcgtagt ccctttcgcc
     5821 ttcacccatt cgccgtcgat caccgcagtg atcgggtctt tctcgaaatc gtgctgggtc
     5881 gcggtgttct tttgcggtac catatccagg tgggcctgca ggatgatccc tttgcggtcc
     5941 tccatcccgg gcgtcgccgg cttgcggatg ataatattgc ctactttgtc gcggaatgtc
     6001 tccagtccgg cctcatcgcc gaattccagc atgaactgct ggattttttc ttcatgcttc
     6061 gaaggcctcg gtacccgggt caacctgtaa aagttctccc ataacgcctt cggttccagt
     6121 ttcttaatct cttcgctcat atcgtaaatg tttcagtgtt tcagtgttcc agtgttccag
     6181 tgttcaattg ttcaattgtt caatagttca attgttcaat agttcaattg ttcaatagtt
     6241 caatagttca attgttatac tgttaaaata ttaaatcgtt aagtcgtcaa atcgtcaaat
     6301 cgtcaaatcg tcaaatcgtc aaatcctcaa atcttcaaat cgtcaaatct tccaaccctt
     6361 gttctcatct tttattcggc aattccagcc cgtattgctt gcgtttgtct tcccttttca
     6421 ggagataccc caggaagatg gcggccattc ccagaccgga aaagatcagc atcggaacgg
     6481 tatagttata tgttgcggtc aggtcgccag cctggatctt ttcagcgacg cccgggttga
     6541 cggctttcag cacgatgccg atcagccagg ggataaacca gaggccgatg ttctgtatcc
     6601 agaaaacaag ggcatacgcc gacccaaggt aacggtcttc cacgatcttt ggcaccgaag
     6661 gccacatgga tgccggaacc agcgaaaaag
//
LOCUS       DAOPNH010000191         6686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13065_length_6686_cov_6.215051, whole genome shotgun sequence.
ACCESSION   DAOPNH010000191 DAOPNH010000000
VERSION     DAOPNH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13065_length_6686_cov_6.215051"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKZ74_10160"
     CDS             <1..415
                     /locus_tag="PKZ74_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPM93162.1"
                     /translation="PRSRKLANPPLMAGFKPFGMPLCESRSVKLQFDEYESFHLVNYK
                     NLQQDQAAGQMGVSRPTFTRIYNRALKKIAKAFAECSSIAIEGGNVEFNKHWYKCRKC
                     FKLIDGLENHTKCKNCPSFNEDELIMLEEINQSIY"
     gene            433..1152
                     /locus_tag="PKZ74_10165"
     CDS             433..1152
                     /locus_tag="PKZ74_10165"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPM93163.1"
                     /translation="MENVNQKEVIPMPRIGDAAPSFKAVTTQGTINFPEDYKGNWVIL
                     FSHPADFTPVCTSEFMTFATMEKQFAEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKG
                     MKNVEVMFPLIEDITMEVAMKYGMIQPGESNTKAVRAVFFVDPKGIIRAIIYYPLSLG
                     RNFDELYRALVAMQAADAFSIATPADWRPGDEVIVPPAGSCGVAKDRMEGKEEMKCYD
                     WFFCTRKISKEEVEKAILKIS"
     gene            1156..1347
                     /locus_tag="PKZ74_10170"
     CDS             1156..1347
                     /locus_tag="PKZ74_10170"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPM93164.1"
                     /translation="MPHLNHTGPEGKGPKTGRKLGLCHKNEKESEAMGTMGEGMGLRR
                     HSDGGKGKGRRKKYDQNND"
     gene            1347..1706
                     /locus_tag="PKZ74_10175"
     CDS             1347..1706
                     /locus_tag="PKZ74_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPM93165.1"
                     /translation="MMKIAVPVNRNGEVDGHFGHCEYYSIYTISSNGEIGLPEIIESV
                     DGCGCKSNIASVLAAKGVKVMLAGGIGGGAVNVLNSEGIEVVRGCSGPSSELVKMFAA
                     GKISDSGETCTHHHHHE"
     gene            complement(1697..2596)
                     /locus_tag="PKZ74_10180"
     CDS             complement(1697..2596)
                     /locus_tag="PKZ74_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93166.1"
                     /translation="MSNKIKNETVYRIDHIPGEEIDVDQEFENIHTHREYDIHGNLLM
                     EITYTRDGALADKILMDYDEKGNLTETRVFDEYDDILERKEAHYFPDGRLQKEITHYL
                     DGSYDLNEYFYDDEGRLTGMKVKDDEDEPDYSERYFYDGEDVVKLERYDDEDELIFSQ
                     EDKFEAGKLSSRTTWSDEDDEPYTLVQNYSVHGHMVEELRYNSDNELVERNIYEEDEN
                     GRVFRLTEENRQRKNTTEFEYDDKGRVTYQKETDLHGDLNHELWRFFSPDGELVRTTV
                     EMVMKPSLEKRAYSLIHKREYYS"
     gene            2711..4831
                     /locus_tag="PKZ74_10185"
     CDS             2711..4831
                     /locus_tag="PKZ74_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPM93167.1"
                     /translation="MKKLLIISVGMMFLTMISCQQQPKFTYPVTAKVDQTDDYFGTTV
                     TDPYRWLEDDNSAETAEWVKAQNAVTFGYLEQIPYRQKIQDRLTKIWDYPKVTAPYKE
                     GGRFYYSKNNGLQNQFVVFSKDALDGEERMVLDPNTFSEDGTVSLSTFSPSDDGKYIG
                     YGISRGGSDWSEYFVMNAGDLTKLDDHLKWIKFSGISWYGDGFFYSRYPEPAKGDELK
                     GMNENSKVYYHKVGDPQEKDELVYEDPAHPNWGFSAYATEDKEVLILTTNESTKGNKL
                     AFRRMADKNKAFTSLVDYFQYDYYPIQHIKGVLYVMTDEGAPKYRLIAIDLNNPAKEN
                     WKVVIPEKEEVLTSISMVGNKIVATYMKDAHDVVQFFDVEGNYLYDLEMPILGSIGGF
                     SGKMTDTFTFYTVTSFTTPATIFKYDLENNVSEEYNRSAIDFDPNLYETRQVFYTSKD
                     GTKVPMFIVHKKGIKLDGNNPALLYGYGGFNVSLTPSFSMSRLILLENGFVFAMANLR
                     GGGEYGEEWHQGGTKMNKQNVFDDFIAAAEYLVANKYTSPKRLAIQGGSNGGLLVGAV
                     INQRPDLFGVALPAVGVMDMLRYHKFTIGKYWAVDYGTSEDSKEMFDYLRKYSPLHSI
                     RNDVEYPAVLVTTADHDDRVVPAHSFKYIATLQENYLGRNPVMIRIETKAGHGGGKPT
                     AMIIEEYADLWAFVMKNLGVTPKY"
     gene            complement(5023..6165)
                     /locus_tag="PKZ74_10190"
     CDS             complement(5023..6165)
                     /locus_tag="PKZ74_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPM93168.1"
                     /translation="MNKIGATGLLAIALLMAACNEKEDVTLIPVFRPTPYEIEIPFGF
                     PTKLNIPDDNPMTVEGIELGRLLFYDGRISGQSEPGKQMSCATCHLQENAFECGLDHP
                     DFPGGKTFGVTGIPTPHYMLPLFNQVWNHSGYLWNGFVNDDNPDAMLRNIEALTYMSI
                     IAPHEMNSDTLSAVKAIKSIPGYANLFKKAFGSEEVTIDRMSKAIAQFIRTFVSADSK
                     FDQYLRGETQLSPSELNGYVLFVTEEGADCFHCHGGGGNPLFTTHLFYNNAKDSVFDD
                     PRDRFSVTGDPMMRGAYKAPSLRNIALTAPYMHDGRFKTLDEVIDFYSEGLVYTGLAD
                     PLMHHLLNGGTRLTPSEKADLKAFLLTLTDETFIKNPALSRPDSLP"
     gene            complement(6158..>6686)
                     /locus_tag="PKZ74_10195"
     CDS             complement(6158..>6686)
                     /locus_tag="PKZ74_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93169.1"
                     /translation="IPETMTWEIFDSLPPGAIDSISFVFGISEEKNQSYMYVNPPESL
                     MFWPDILGGGYHYLKLNGKWKNEENQLSPFNFHLGIGQVYDTAGHITGFIQNYFRTGA
                     RLPVYSSYALVVEPGETTSVTLAMDVASWFETPDIWDFNYWGGDIMQNQEALHAGCRN
                     GFDIFSLMSPLFINE"
BASE COUNT         1778 a         1551 c         1691 g         1666 t
ORIGIN      
        1 accgagaagc cgtaaacttg caaatccgcc gctgatggca ggatttaagc cgttcgggat
       61 gccgctgtgc gagtcccgtt cggtcaagct tcagtttgat gagtacgaga gttttcatct
      121 cgtcaactat aaaaatttgc aacaggatca agcagccggg cagatgggtg tgtcgaggcc
      181 cacttttacc cggatataca acagggcttt gaaaaaaatc gccaaggctt ttgccgagtg
      241 ttcttctatt gcgatagaag gggggaatgt cgaatttaac aaacactggt acaaatgcag
      301 aaaatgtttc aagctgatcg acggactgga gaatcatacg aaatgtaaga attgtccatc
      361 tttcaatgaa gacgagctga tcatgcttga agagatcaat caatctattt attaatcaag
      421 aattataaaa aaatggagaa tgtaaatcaa aaagaagtca ttccgatgcc gcgcatcggt
      481 gatgcagcgc cttctttcaa ggcggttacc acacagggaa ctatcaattt tcctgaagat
      541 tataaaggga actgggttat tttgttcagc catccggcag attttacccc ggtatgtact
      601 tctgagttta tgacgtttgc cacgatggag aagcaatttg ccgaagccaa ctgtaaactg
      661 gtaggtttgt cggtcgacgg cctttacagc cacatcgcct ggttgcgcac gatcaaagaa
      721 aagatcgagt acaaagggat gaaaaacgtc gaggtgatgt ttccgctgat cgaagacatt
      781 accatggaag tagccatgaa atacggtatg atccagccgg gagagagcaa taccaaagcg
      841 gtgcgggcgg tatttttcgt tgatcccaaa gggatcatcc gggccattat ttactacccg
      901 ctgagcctgg gccgcaactt cgacgagctg taccgggctt tggtcgccat gcaggctgcc
      961 gatgctttct cgatcgctac tccggccgac tggcgcccgg gcgacgaggt gatcgttccg
     1021 ccggcaggaa gctgtggcgt tgcgaaagac agaatggaag gcaaagagga gatgaaatgt
     1081 tacgactggt tcttctgcac caggaagatc agcaaggaag aggttgaaaa agctatcctg
     1141 aaaatttcct agaaaatgcc acacctgaat cacaccgggc ctgaaggtaa aggacccaag
     1201 accgggagga aactgggcct ttgccataaa aatgaaaaag aaagcgaagc gatgggaacg
     1261 atgggtgaag ggatggggct tcgcaggcat tcagatggtg ggaaaggaaa aggcagaaga
     1321 aaaaagtatg atcaaaataa tgattgatga tgaaaatagc tgttcctgta aacagaaacg
     1381 gagaggttga cggacatttc ggacactgtg aatattatag tatttacact atctcttcaa
     1441 atggtgaaat agggcttccg gagatcattg aatcggtcga cggatgcggt tgcaagtcga
     1501 atatcgcatc ggtcctcgcc gccaaaggag tgaaggtgat gctggccggc ggcatcggag
     1561 gcggtgcggt gaatgtgttg aacagtgaag ggatagaagt ggtcaggggt tgttccggcc
     1621 cctcttcgga gctggtgaaa atgtttgcgg caggtaaaat ctccgacagc ggggaaacct
     1681 gtacgcatca ccaccatcat gaatagtatt ccctcttgtg gatcagtgaa taagccctct
     1741 tttccaggga gggcttcatg accatttcaa ccgtcgtcct gaccagttca ccatccgggc
     1801 tgaaaaaccg ccacaactca tggtttaggt caccgtggag atcggtctct ttctggtagg
     1861 tcacacggcc tttatcgtca tactcaaatt cggtcgtgtt cttccgctgg cggttttctt
     1921 ccgtcagccg gaagaccctg ccgttctcat cttcttcata aatattcctc tctaccagct
     1981 cattatcaga attataacga agctcttcca ccatgtggcc gtgaactgaa taattctgaa
     2041 ccagggtata aggctcatca tcttcatccg accaggtagt tcggcttgac agttttcccg
     2101 cttcaaattt gtcttcctgg ctgaaaatca attcgtcttc atcgtcataa cgctccagct
     2161 taacaacatc ttccccatca taaaaatacc tttccgaata atcgggttca tcttcatcat
     2221 ctttaacttt catgccggtc aaacgccctt catcatcata aaaatattca ttcagatcgt
     2281 aagaaccgtc gagataatgg gtgatctctt tctgaagacg gccatccgga aagtaatgcg
     2341 cctcctttct ttccaggata tcatcatatt catcaaagac gcgtgtttcg gtcaaatttc
     2401 ccttctcatc ataatccata aggatcttat cggccaaagc cccgtcgcgc gtgtaggtga
     2461 tttccatcaa caggttgccg tgaatgtcgt attcccggtg tgtatggatg ttttcaaatt
     2521 cctgatccac atctatctct tcgccgggga tatgatcgat acgataaacg gtttcgtttt
     2581 ttattttgtt actcattgcc tgatatcttc atttggtaaa ctcaatccgt gatttccgcc
     2641 aaaattggtt atttttgccc taatcaccaa aaagtttatc aaccctttat tcaccgtaaa
     2701 aaagaatttt atgaagaagt tgttaattat tagtgtcggt atgatgtttt taacgatgat
     2761 ctcgtgccag caacagccaa agtttactta tccggtcacg gccaaagttg accagacaga
     2821 tgattatttc ggaacaacgg tgactgatcc ataccgttgg ctggaggatg acaactcggc
     2881 agaaacagca gaatgggtca aagcccagaa tgcggttact tttggatatc ttgagcaaat
     2941 cccttaccgg cagaagatcc aggaccggct gacaaagata tgggattacc cgaaagttac
     3001 ggcgccttac aaagagggcg gccggtttta ttattcaaaa aacaacggcc ttcagaacca
     3061 gtttgtcgtt ttttcaaagg atgcccttga cggcgaagag agaatggtgc tcgatccgaa
     3121 tactttctcc gaagacggaa cagtttccct gtcgacgttc tccccgtcgg atgacggaaa
     3181 atacatcggg tacggcattt ctcgcggagg ttccgactgg agtgaatatt tcgtgatgaa
     3241 tgccggcgac ctcaccaaac tcgacgacca tttaaaatgg atcaagttct ccggaatttc
     3301 gtggtatggc gacggatttt tctacagccg ctacccggaa ccggccaaag gcgatgagct
     3361 gaaggggatg aatgagaaca gcaaagtcta ttaccacaag gtcggcgatc cgcaggaaaa
     3421 ggatgaactg gtttatgaag atccggccca tccgaactgg ggtttttcgg cctatgccac
     3481 ggaagacaaa gaggtactga tccttaccac caatgaatcg accaaaggga acaagctggc
     3541 tttccgcaga atggctgata aaaacaaagc ctttacttcg ctggtcgact atttccagta
     3601 cgattattat cccattcagc atattaaagg tgttttatat gtgatgacgg atgaaggagc
     3661 gccgaagtac aggctgatcg ccatcgacct gaacaatccc gcaaaggaaa actggaaagt
     3721 ggtcatccct gaaaaagagg aagttctgac cagtatttcc atggtcggca ataagatcgt
     3781 tgccacttac atgaaagatg cccatgatgt ggtgcagttc tttgatgttg aaggaaatta
     3841 cctttacgac ctggagatgc cgatcctggg atcgatcggt ggcttctcgg gtaagatgac
     3901 cgatacgttc actttctaca cggtcaccag ctttactact ccagccacga ttttcaaata
     3961 cgacctggag aataatgttt ctgaagaata caaccgttcg gccattgatt ttgatccgaa
     4021 tctttatgag accaggcaag ttttttatac ctccaaggat ggcaccaaag ttcccatgtt
     4081 catcgttcac aagaagggga taaagctgga tggaaataac ccggcattgc tttacgggta
     4141 tggcgggttc aatgtcagcc ttacgccttc attcagcatg agccggctga tcctgctgga
     4201 gaacggcttt gtgtttgcca tggccaacct gcgcggtggc ggcgaatacg gtgaagaatg
     4261 gcaccagggc ggcacaaaaa tgaataagca gaacgtattc gacgacttca tcgctgcggc
     4321 ggaatacctg gtagccaaca agtacacttc gccaaagagg ctggcgatcc agggcggttc
     4381 caacggcggg ctgctggtcg gcgccgtgat caaccagcgt cccgacctgt tcggtgtagc
     4441 gcttccggcc gtcggtgtga tggatatgct tcgttaccat aagttcacca tcggcaaata
     4501 ctgggcagtg gattacggta cgagcgaaga cagcaaagag atgttcgatt acctgcgcaa
     4561 atattcaccg ttgcattcga tccggaacga tgtggaatac ccggcggtgc ttgtcactac
     4621 cgccgatcac gacgaccggg tggtaccggc ccattccttc aaatacattg ccacgctgca
     4681 ggagaattac ctgggcagga acccggtgat gatccgcatc gagaccaaag ccggccacgg
     4741 cggcggcaaa ccgacggcga tgatcatcga ggaatatgct gatctctggg cgtttgtgat
     4801 gaaaaacctg ggcgttacgc cgaaatatta gcagggaaaa gggaaaagcg aaaagggaaa
     4861 aagtctgata tttcaaattg atggcctgat attagttcgt gctccaacga ctaaaaccgt
     4921 tggttcctat ggttttatga aaatgggaac cgacggcttc agtcgtcgga ctaccaatta
     4981 aatgcaaata tttgtatttt tgagacagtc actgcgtcaa acctaaggta gtgaatccgg
     5041 tctcgaaagg gccggatttt ttatgaaagt ttcatcggtg agggtcagca ggaaggcttt
     5101 caggtcagct ttttctgaag gcgtaagacg ggtgccaccg ttcagcaaat ggtgcatcag
     5161 cggatcggcc agcccggtgt agacaagccc ttcggaatag aaatcgatga cttcgtcgag
     5221 ggttttaaat cttccatcgt gcatatatgg agccgtcagg gcgatgtttc ggagcgaagg
     5281 cgctttgtat gcgccccgca tcatcgggtc gccggtcacg gaaaagcggt cgcgcggatc
     5341 atcgaaaaca gagtccttgg cgttattgta aaacagatgg gttgtaaaca gcggatttcc
     5401 gccgccaccg tggcaatgaa agcaatcggc cccctcctcc gtaacaaaca gaacatagcc
     5461 gttcaattct gacggagaga gttgtgtctc accacgaagg tactgatcaa actttgagtc
     5521 ggcggaaacg aaagtcctga tgaactgtgc aatagctttg ctcatccggt cgatcgtaac
     5581 ttcctcactg ccgaacgctt ttttgaaaag atttgcatac cccgggatgg attttattgc
     5641 cttgacagcc gaaagggtgt cgctgttcat ttcgtgcggc gcgatgatgc tcatataggt
     5701 cagcgcttcg atgttgcgaa gcatcgcatc agggttgtca tcgttaacaa acccgttcca
     5761 caggtatccg gaatggttcc agacctggtt gaacagtggc agcatgtaat gcggggtagg
     5821 gatgccggtc acgccaaagg tctttccacc cgggaaatcg ggatgatcga ggccgcattc
     5881 gaaggcgttt tcctgcaggt ggcaggtagc gcagctcatt tgtttgcccg gttcactttg
     5941 tccggatatc ctgccatcat aaaacaacaa ccggcctaat tcaatcccct ccaccgtcat
     6001 tggattatca tccgggatat tcaatttggt cgggaagccg aagggtatct cgatttcata
     6061 aggagttggc ctgaagaccg gtatcaaagt cacatcctcc ttttcattgc atgcagccat
     6121 aaggagggca attgccagta agccggtggc accaatctta ttcattaatg aacagaggtg
     6181 acatgagtga gaaaatgtca aatccattac ggcaaccagc atgcaaagct tcctggtttt
     6241 gcatgatatc gcctccccag tagttgaaat cccagatatc cggtgtttcg aaccagctgg
     6301 ctacatccat ggccagggtt accgaggttg tttctcccgg ttcaacgacc agcgcataag
     6361 atgaataaac cggcagcctg gcgccggtgc ggaaataatt ctggataaac ccggtgatat
     6421 ggcctgccgt atcgtaaacc tgcccgatgc caagatgaaa gttgaacggg ctgagctgat
     6481 tttcctcatt tttccatttt ccgttcagtt tcaggtagtg gtaaccgccg cccaggatat
     6541 cgggccagaa catcagcgat tcgggtggat tgacatacat atacgactgg ttcttttctt
     6601 cgctgatccc gaaaacaaag ctgatcgaat caatcgcacc cggtggcagg ctgtcgaata
     6661 tctcccaggt catggtttca gggatg
//
LOCUS       DAOPNH010000192         6557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13429_length_6557_cov_5.388803, whole genome shotgun sequence.
ACCESSION   DAOPNH010000192 DAOPNH010000000
VERSION     DAOPNH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_6557_cov_5.388803"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKZ74_10200"
     CDS             <1..752
                     /locus_tag="PKZ74_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93170.1"
                     /translation="AIEAYEFAIAIDERFSSAYFNLGNAYANMGEYRLSIKNYKEALK
                     YEEPQALTYYYIGECYEKLDRFPDAIQYYRLALEIDPALAEAWMGLGVCLDETGDPHT
                     AARHMERALDLEDDNAENWYIYGDLKYKLNDKEAAVRAFEKVSALDPMHSDIWLDLAD
                     IYLETGEMTKAFDTLEEGIFQQPKNASLLYRMSSLLFRNGKQKQGLAVLSEGLETDFD
                     KHSDLFEFAPELEQNRDVQELIQLHYNKMHN"
     gene            759..1511
                     /gene="aroE"
                     /locus_tag="PKZ74_10205"
     CDS             759..1511
                     /gene="aroE"
                     /locus_tag="PKZ74_10205"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338490.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPM93171.1"
                     /translation="MRIFGLIGYPLGHSYSKQYFDRKYLAENITDCRFENFPLNDISL
                     MNDLIINNPECAGFNVTIPYKSRIMEYLDWIEPGAREIGSVNCIKVEREGGQTIRKGF
                     NTDMPAFRETLRMFVPDAGISGALVLGSGGAAKSVCQALRETGIEPVIISRSAGMGQL
                     TYHSLSSEIIKKNRLIINTTPLGMWPETEAFPPLPYHLITSHHIFYDLIYNPEMTTFL
                     MKGAEAGAKIISGLHMFHIQAELSWKIWNSGL"
     gene            complement(1506..1769)
                     /locus_tag="PKZ74_10210"
     CDS             complement(1506..1769)
                     /locus_tag="PKZ74_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPM93172.1"
                     /translation="METKYKSEFFVTGEELLGKVRELIHEGNVRKIIIKNEKGETYLE
                     IPVTIGVIGAILAPVFAAIGALAALAANFTIVVERKDDEPAAS"
     gene            1848..3089
                     /locus_tag="PKZ74_10215"
     CDS             1848..3089
                     /locus_tag="PKZ74_10215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM93173.1"
                     /translation="MPEIKTTEIAILGAGAAGLTAAIRLGQLGIPCLLIDKGEFPRSK
                     ICGDGLSGKTLSALKRLNPQYIDEIGDLATATSSQAARFFSPRGKMLQLGFRSEDLSH
                     PPGLICERFHFDHFLYSKAISSGKCGFVANTRISQITRENGQMILSDPDGEKQVRCSL
                     LLFGAEANKGLIRQFHPGYPVTIEEGIGIRGYFDRVMGADQLHAIEIHFFRELLPWYF
                     WIFPFADGSANVGLALPESQARKLDKSLKQILFSLIADKPHLKMRFSQASLKGKLEAH
                     RLPVYASGGCPVAGDGFMLIGDAARLIDPFTGEGIGNAMVSGFKAAETASKCYKNGDF
                     SFKYTGEYENEIREKLYPELDLSLKLQNLARKQRLLNLVIGKASKDEKLRDLMSDMLY
                     STYTKSKLNHPWFYLKLLTGI"
     gene            3145..5115
                     /locus_tag="PKZ74_10220"
     CDS             3145..5115
                     /locus_tag="PKZ74_10220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM93174.1"
                     /translation="MKLSVIIVNYNVRYFLEQCLYSVRKAAEGIDTEVFVVDNNSVDG
                     SVEMVRKKFTEVILIENKENTGFSKANNQAIRKACGEFILLLNPDTVVEDDTFTKAIG
                     FMESTPDAGGLGVKMIDGKGNFLPESKRGLPTPSVAFYKIFGLSKFFPRSRIFGRYHL
                     GYLDKDEIHPVEILSGAFMLMRKSALDKVGLLDETFFMYGEDIDLSYRILKGGFKNYY
                     FPETRIIHYKGESTKKGSINYVLMFYNAMIIFARKHFASKQARTFSFLIHSAIYFRAF
                     LAIISRFFRQVMLPVADAILIYGGLYFIINYWEDKVIFPQGGGYPPGYFSFYVPGYIL
                     IWIISGFFAGTYDRPYKISRIFRGISVGTVIILVAYALLPENLRFSRAIILMGSVWAV
                     IVMVISRTICQFASDRTLSFTPQKNRRFVIVGDRHEAGRVRDLLHSSYGTPGFIGLVS
                     ANPVATRNEDFIGSLVQIKEIIRVYRIDEVIFCSKSMTHQSIIDQMSALQDTGVDYKI
                     APEDSLSIIGSNSINTAGDLYTVNINAITNTENRRNKRLLDLAISLVAIILLPFLLFL
                     VHQPFGMVRNIFLVVVSRRSWVGYIHDDHYDIERLPHIRKGILHPLDAFGKDHISDEI
                     RQRLNLIYARDYSTATDLNIVFKGIRNLGRKS"
     gene            5212..6492
                     /locus_tag="PKZ74_10225"
     CDS             5212..6492
                     /locus_tag="PKZ74_10225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPM93175.1"
                     /translation="MARFEILMPKMGESIIEATITKWLKKAGDPIAEDEAIAEIATDK
                     VDSEIPSPVDGVVEKVLFNEGDVVPVGKVIAVINLGGDEETGEKEEKETESQGDKETA
                     GKEDKETVKQGDGRFYSPLVKNIARQENIPLNELDKISGSGKEGRVTKDDILNYAQLR
                     KQGAPVPEVSAHPPTIKTPQAVEVPVVTTEAGDEVFEMDRMRRLIADHMVMSKQVSPH
                     VTSFIDVDVSRIVTWREKVKDDILARELEKITYTHILMETCARVLKEFPRVNASVDGY
                     KIIHRRRINIGMATALPNGNLIVPVIKDADQKSLLGIIKNVNDLANRARKNSLLPDEI
                     QGGTFTITNFGTFDSLSGTPVINQPQVAILGTGAIRKKPVVVESEYGDIIAIRHIMIL
                     SLAYDHRVVDGALGGMFLKRMKELLENWDMSRKF"
BASE COUNT         1804 a         1504 c         1603 g         1646 t
ORIGIN      
        1 gggccatcga agcctatgaa tttgccattg ctatcgacga gcggttttca tcggcctatt
       61 ttaacctggg caatgcttat gcaaatatgg gggaatacag gctgtcgata aagaattaca
      121 aagaggcatt gaaatatgaa gagccacagg ctttgacata ttactatatt ggggaatgtt
      181 atgaaaagct ggatcgcttc ccggatgcta tccagtatta ccggctggcg ctggagatcg
      241 atccggcttt agcggaagcc tggatgggcc tgggcgtttg cctcgatgaa accggcgatc
      301 cccacacagc ggccaggcat atggaacggg cccttgacct ggaggatgac aacgcggaga
      361 actggtacat ttacggtgac ctgaaatata agctcaacga caaggaggca gccgttcggg
      421 cttttgaaaa agtcagcgca cttgacccta tgcacagtga catatggctt gatctggccg
      481 atatttacct ggaaaccggt gaaatgacca aagcattcga cacacttgaa gaaggcattt
      541 tccagcagcc taagaacgca tcgttactct accggatgtc atcgctattg ttccgtaacg
      601 gaaaacaaaa gcagggactg gctgttttat cggaaggact tgagaccgat ttcgacaaac
      661 actccgactt gttcgaattt gccccggaac tggaacagaa cagagatgtt caggaactta
      721 tccaactgca ttacaataaa atgcataact gatcccgcat gaggattttc ggcctcatcg
      781 gttaccccct cggtcactct tattcaaaac aatattttga tcgtaaatat cttgcggaaa
      841 atataactga ttgcaggttt gaaaatttcc cacttaatga tatttcgtta atgaatgatc
      901 tgattatcaa caatccggag tgtgccggtt ttaatgtaac cattccctat aagtcacgaa
      961 taatggaata tctggattgg atagaacctg gggcccggga gatcggatcg gtgaattgca
     1021 ttaaagttga gagggaaggc gggcaaacca tccggaaggg ttttaatacc gatatgcctg
     1081 cttttcgtga aacactccgg atgtttgtcc cggatgcagg tatttcaggc gccctggtcc
     1141 tgggatcagg tggggcggcg aaatctgtct gccaagcgct cagggagacg ggcattgaac
     1201 cggtcattat ttcacgttcg gcgggtatgg gtcaacttac ctatcattct ttatcatccg
     1261 aaatcatcaa aaaaaacagg ctgatcatca atacgacacc tctcggcatg tggccggaaa
     1321 cagaagcatt tccacccctt ccctaccact taataacttc acatcacatt ttttatgacc
     1381 tcatctacaa tcctgaaatg acaacatttc tgatgaaagg agctgaagca ggggcaaaaa
     1441 ttatcagtgg ccttcatatg ttccacattc aggctgaatt aagctggaag atctggaatt
     1501 ccggcctatg aggcggccgg ttcgtcgtct ttccgctcga ccacaatggt aaaattggcg
     1561 gccagggcgg ccagggcccc aatagccgcg aaaacaggcg ccagtatcgc gccgatcacc
     1621 ccgatggtaa ccggtatctc caggtaggtt tccccctttt cattcttgat aatgattttc
     1681 ctgacattgc cttcgtgaat aagttccctg acttttccca ggagctcttc tcccgtcaca
     1741 aagaattccg atttatattt agtttccatc tttttaaaat ttaattcaaa attattcaaa
     1801 ttatcagcgt ggctgatttt atctaatttt ggcaaccact aaattccatg ccagaaatta
     1861 agacgacaga gatagctatt ctgggggccg gcgcagcggg cctgacggcg gccatccggc
     1921 ttggccagct tggaataccg tgcctgttaa tcgataaggg ggaatttccc cgctcaaaga
     1981 tttgcggtga cggcctgagc ggaaaaaccc tcagcgcatt aaaaaggctt aatcctcagt
     2041 acattgatga aattggcgac cttgctacag ccacatcttc gcaggcagcc cgattttttt
     2101 ccccccgggg gaagatgctt caactcgggt tcagatcgga agatttgtca catccaccgg
     2161 gtttgatttg cgagagattt catttcgacc attttcttta cagcaaggcg atctcttccg
     2221 gcaaatgcgg gtttgtggct aacacacgaa tttcacaaat tacccgggaa aacgggcaga
     2281 tgatactttc agaccctgac ggtgaaaaac aggtccggtg ttcactcctg ctctttggcg
     2341 ctgaagccaa taaggggcta atcaggcagt ttcatcccgg ttatccggtt accatcgaag
     2401 aagggattgg gatcaggggg tattttgacc gggttatggg tgctgatcaa ttgcatgcca
     2461 ttgaaattca ctttttcaga gaacttctcc cctggtattt ctggatattt ccttttgcag
     2521 acggctcggc caacgttggg ctggctcttc ccgaatccca ggcacggaag ctggataaaa
     2581 gtcttaaaca aatcctattc agcctgatcg cagacaaacc ccatctaaaa atgaggttca
     2641 gccaggcatc gttaaaaggc aaacttgaag cccaccggct tccggtttac gcttccgggg
     2701 gttgtcctgt tgccggagat ggatttatgc tcataggaga tgcagcccgt ctcattgacc
     2761 cttttactgg ggaaggaata gggaatgcca tggtcagcgg gttcaaagcg gcagaaacag
     2821 cctcaaagtg ttataagaat ggagacttta gcttcaaata caccggggaa tacgaaaatg
     2881 aaatcaggga aaagctttac cctgagttgg atctcagcct taagcttcag aatctggccc
     2941 gtaagcagcg ccttcttaac ctggtaattg gcaaagcttc aaaagatgaa aagctcaggg
     3001 atcttatgtc ggacatgtta tacagcacgt ataccaaaag caaactgaac cacccctggt
     3061 tttatcttaa attactgaca ggtatttaat aaaccgcaaa atcatcgcag gatttgtatt
     3121 tttgtcgtgt tattggattc taggatgaaa ttgtcagtca tcatcgtcaa ttacaacgtc
     3181 aggtatttcc tggaacaatg tctttattcg gtaagaaagg ccgcggaagg cattgatacg
     3241 gaagtgtttg tggtggataa caattcggtt gacggatcgg tggaaatggt caggaaaaag
     3301 tttaccgaag tcatcctgat cgaaaacaaa gagaacacag gattttccaa agccaataac
     3361 caggcgatcc ggaaagcgtg cggggagttt atcctcctgc tcaatccgga tacagttgtt
     3421 gaggacgata cctttaccaa agctatcggg tttatggaat cgacgccgga tgccggtggc
     3481 ctgggggtta agatgatcga cggaaaaggt aatttccttc cggaatccaa aaggggactt
     3541 ccaacccctt cggtagcgtt ttataagatc ttcgggcttt caaagttttt cccccgatcg
     3601 agaatctttg gccggtatca tctgggttac cttgataagg atgagattca cccggttgag
     3661 atcctttccg gtgcatttat gctgatgcgg aaatcggctc ttgacaaagt cgggttgctg
     3721 gatgaaacat tttttatgta cggagaagat atcgacttgt cgtaccggat attgaaaggc
     3781 ggctttaaaa actattattt ccccgagacc cggatcattc attataaagg tgaaagtact
     3841 aaaaaaggga gcatcaatta tgtgctgatg ttttacaatg cgatgatcat tttcgcaaga
     3901 aaacattttg catccaaaca ggccaggact ttttcattcc tgatacattc ggcgatctat
     3961 ttcagggcat tccttgccat catcagccgt tttttcaggc aggtcatgtt accggttgcc
     4021 gatgccattc tgatatatgg tggtctctat tttataatca attactggga agataaggtg
     4081 atctttcccc agggtggagg atacccacca ggatattttt ctttttacgt gcccggatac
     4141 atcctgatct ggataatttc cggattcttt gccgggacct atgacaggcc ctataagatt
     4201 tcccggatat ttcgcggcat ttcggtcggt acagtgatca tcctggtcgc ttatgccctt
     4261 cttcctgaga acctccgttt ctccagggct atcattttaa tgggttcggt atgggcagtc
     4321 atcgttatgg tgataagccg cacgatctgt caattcgcct ccgaccggac cctctccttc
     4381 acaccacaaa agaacaggcg gtttgtcata gtgggcgatc gccatgaagc cggcagggtc
     4441 agggatcttc tgcacagttc gtacggaacg ccgggattta tcgggctggt atccgctaac
     4501 cccgtggcaa caagaaatga agatttcatc ggttcgctgg tgcaaatcaa ggagattatc
     4561 agagtttaca ggatcgatga agtcatcttt tgttccaaaa gcatgaccca ccagtcaata
     4621 attgaccaga tgtcggcatt gcaggacacc ggcgtggatt acaaaattgc cccggaagac
     4681 agcctctcta ttatcggcag caactccatc aacaccgccg gagatcttta caccgttaac
     4741 atcaatgcca ttaccaacac agaaaaccgc cggaacaagc ggttactgga tttggccatc
     4801 agcctggttg ccattattct gctccccttt ctccttttcc ttgttcacca accgttcggc
     4861 atggtccgga atatattcct ggttgtggta agccggcgtt cctgggtagg ctatattcat
     4921 gacgaccatt acgatatcga gcggttgccg catatccgca aaggaattct ccatcctttg
     4981 gatgcattcg gaaaagatca tatttccgat gagatcaggc aaaggctaaa tctgatctat
     5041 gccagggact acagtacggc caccgatctg aacattgtct ttaaaggaat caggaacctg
     5101 ggcaggaaaa gctgaaccgg cccgaacaat ccgcccatca ctttgttatt aattgttaac
     5161 tttgcagact gctgtagaat ctgctgcagc atatcattaa acataaactc aatggccaga
     5221 tttgaaatcc tgatgcccaa gatgggcgaa agcatcatag aagcaaccat tacaaaatgg
     5281 ctcaaaaagg caggcgaccc gatcgcagaa gatgaagcga ttgctgaaat tgcgacagac
     5341 aaggtcgact ccgaaatacc ttcaccggta gacggtgtgg ttgaaaaagt tttattcaac
     5401 gaaggcgatg tcgtcccggt gggtaaagtg atcgccgtta ttaatttagg tggagatgag
     5461 gaaacagggg aaaaggaaga aaaggagaca gagagtcagg gcgataagga gacagcagga
     5521 aaggaagaca aggagacggt gaaacaagga gatgggaggt tttattctcc attggtgaaa
     5581 aatatcgcca ggcaggagaa tattccatta aacgagcttg acaaaatttc ggggagcggg
     5641 aaggaaggca gggtaacaaa agatgacatc ctgaactatg ctcaattgcg caagcaagga
     5701 gctccggtac cggaggtatc agcccatccg ccaacaatta aaaccccgca ggccgttgaa
     5761 gtgcctgtag tgaccaccga ggccggggat gaggttttcg agatggaccg tatgagaagg
     5821 ctgatagccg accatatggt gatgtcgaaa caggtttcac cgcatgtcac ctcttttatc
     5881 gatgtagacg tgagccggat agttacctgg agggaaaagg ttaaagacga tatcctggcc
     5941 agggaactcg aaaagatcac ctacacgcac atcctgatgg aaacatgtgc cagggtgctc
     6001 aaagaattcc cgagagtaaa cgcttcagtc gacggttata agatcatcca tcgcaggcga
     6061 atcaatattg gtatggcgac ggccctgccc aacggcaacc tgatcgtacc ggtgatcaaa
     6121 gacgccgacc agaagagcct tctgggaatc atcaagaatg tgaatgacct ggccaaccgg
     6181 gccagaaaga acagcctcct gccggatgag atccagggag gcacatttac aatcaccaat
     6241 ttcggcacat tcgacagcct gagcggcacc ccggtgatca accagcccca ggtggccata
     6301 ctcgggaccg gcgctatccg gaagaaaccg gttgtcgtgg aatcggaata cggcgatatc
     6361 attgccatcc gtcatatcat gatcctgtcg ctcgcttatg accacagggt cgtcgacggc
     6421 gccctgggcg gaatgttcct caaacgaatg aaggaactgc tggaaaactg ggatatgagc
     6481 aggaaatttt aaactttaaa tcttaagggg acctctaata actgatattt gacataaaaa
     6541 ttgaaataaa aaaatac
//
LOCUS       DAOPNH010000193         6532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13499_length_6532_cov_7.176934, whole genome shotgun sequence.
ACCESSION   DAOPNH010000193 DAOPNH010000000
VERSION     DAOPNH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13499_length_6532_cov_7.176934"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..2062)
                     /locus_tag="PKZ74_10230"
     CDS             complement(149..2062)
                     /locus_tag="PKZ74_10230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM93176.1"
                     /translation="MKYIRLMMMVALPAILITLLAACQQQPVIMKAKQIPLEDFFKNP
                     EKSSYQISPGGTYFSFKAPYQDRMNIFVQKIGTDSAIRLTSEIDRDIMGYFWPNDEQI
                     LYLKDMGGDENFRLYGVNADGSNLKCFTDFEGVRTDIIDDLPEFPDHVIIGMNKRNPQ
                     VFDPYRLNIRTGEMEMLAENPGNIQGWIFDHDGKLRAATAIVDGINSQILYRETEKDE
                     FVPVLTTNFKETLSPVFFTFDNKNVYAISNLGRDKAAAVVFDIKNGKEVEVLYENPDY
                     DIDNIFFSRKRKVLTAANYYGDKRNRHFFDEETRLLFERLNKDLGKYELGISAITDDE
                     NKLIVRTYSDKSLGAYYIFDREADKLTNITDVSPWIDENEMAEMLPVRYQSRDGLTIQ
                     GYLTLPKGYTMETAKKLPVILNPHGGPWARDNWGFNPEVQFLANRGYAVFQMNFRGST
                     GFGRKFWEASFKQWGRTMQDDITDGAKWLISKGIADPDRLVIYGASYGGYATLQGIVK
                     DPGLYAAAVDYVGVSNLFTFMKTIPPYWEPFLQMMYEMVGNPVTDSLRMAETSPVLNA
                     DRIKTPLFIAQGANDPRVNKAESDQMVEALKARGIIVEYMVKDNEGHGFYNEENRFDF
                     YRAMEKFLAENVK"
     gene            complement(2167..3795)
                     /locus_tag="PKZ74_10235"
     CDS             complement(2167..3795)
                     /locus_tag="PKZ74_10235"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93177.1"
                     /translation="MRIILLILISFLINFHSTAQPREFEWASQCGNPSNTTDAKTTLA
                     GADNGYFYMAGEFLGTVQFGNKSLVSAGGTDIWLVKNTPEGAPVWSIRMGGNDDDFVW
                     KIVSDNEDNVTMTGYYYGTIQIGNDIYTSQGSQDLFIARFDGEGNYLWSYTLGGGMAD
                     YIRGLATDSGGNLFLAGHFYDSFIVGDTTVTAARGSDAFVIKFSPDGQPAWVTTAGGS
                     SSDQASSVTIDSEGNILLAASFYYDITFGDTTLHTPNPVGVAIARYFPDGGLDRVFQL
                     NGTYLTTENLIRAGLEGQFYVAGNFSEKLFFGNKTFDAGEFNQDIFTAAFGQDMELLW
                     ARHGFSTASDQVVGLDTDPYGNVYIAGHYLDTIRFDQLKLQYTLCCGSREIFIVSYSS
                     GGAAVWGEQISGARANLQSIEMIENGKMILSGMFTEDLAFGPHVLSHWEGFRNYASSL
                     ETGLITGTPAINNSLANIVLSPNPARTKIQIHSAKPLTGMNYTVFKLNGSVAMEGAMT
                     SNSIDIQNLAPGLFLLRIWNEQEDLTLTAKFIRQ"
     gene            complement(3911..4501)
                     /locus_tag="PKZ74_10240"
     CDS             complement(3911..4501)
                     /locus_tag="PKZ74_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPM93178.1"
                     /translation="MKDIHDLLKIKTNNIKPARGKILISEPFLLDYFFKRSVILLAEH
                     NDEGTFGLIVNKPVKARFNDVVKGFPEFNSRIFLGGPVQSDSVFFIHTMGEEIEESIE
                     IIPGIYWGGDIERVKEMIGLSLIKPSQIRFFIGYSGWSPNQLDEELERNSWVVTNLTA
                     DDILRTTPSMLWSRSLRRLGQDYAHWVKFPDDPTDN"
     gene            4611..5954
                     /locus_tag="PKZ74_10245"
     CDS             4611..5954
                     /locus_tag="PKZ74_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93179.1"
                     /translation="MREEFPVFTYESYHYEFSGETLALKFTFNLSGKYFFHPLVVIPV
                     KNFFRPQNAFKAGELDALVFHAGMIELISYWKAACPATVFIKPHSLTGEQAAFWKKLY
                     YHGLGEFFYINSIPADEGSFMNLHATGPNPLMPFDYSGTGRMVPVGGGKDSAVTIGLM
                     EKAGLDWLPFALSPVSATGEVIRTTGKTRNDTLVFERIIDPMLIELNNRGFLNGHTPF
                     SALLAFYSLLAAYLTGKKDIVLSNESSANEPTVPGTGINHQYSKSFGFEKDFREYVLK
                     NISSGFNYFSLLRPLSELQIARLFSGMPKFFPHFKSCNAGSKAGIWCGRCPKCLFTFI
                     ILSPFLEPGELTRIFGKNLLNDASLAGYLDELTGESPVKPFECIGTVDEVNTALAMTV
                     KHYEPEAMPYLLGISSGRINKADSDLFVDIEKQKMLKDEHFVPVEYSNLLEEVLR"
     gene            6059..>6532
                     /locus_tag="PKZ74_10250"
     CDS             6059..>6532
                     /locus_tag="PKZ74_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPM93180.1"
                     /translation="MTVGIADRDAYLAQKIEDQAELAGIDLHLGEKYLDALNDYSLVI
                     KSPGVGLPENLSLRPGIVITSQTRLMLEAYHRQIIGITGTKGKSTTSSLVHHLLANAG
                     VSSVLVGNIGLPPFDYLDRIGPDTKVVYEMSSHQLEDIGIAPHIAVLLNLFPEHLD"
BASE COUNT         1583 a         1636 c         1584 g         1729 t
ORIGIN      
        1 gatttaaggg ggttatggcg tttaaggggg tatggtgttt tcatggcttg agattttaaa
       61 ggtaaaagta atataaaatc tcaaataaca gggtttccaa ccctggggtt ggaaaccctt
      121 tttaacccat taaaaaaatc ttttatattt acttcacgtt ctccgcaagg aatttctcca
      181 tcgcacggta gaaatcgaag cggttctctt cgttgtagaa gccgtgccct tcgttgtctt
      241 tcaccatata ttccacaatg attccgcggg ctttcagcgc ttcgaccatc tggtcggatt
      301 cggctttgtt aaccctcggg tcattggcgc cctgggctat gaaaagcggt gttttgatcc
      361 tgtccgcatt gagaacagga gacgtttcgg ccatccgcag gctgtcggtc accgggttgc
      421 ccaccatctc gtacatcatc tgcaggaacg gctcccagta aggcgggatt gtcttcatga
      481 aggtgaagag gttggaaacc cccacgtaat caactgccgc agcatacaag ccgggatcct
      541 tgacaattcc ctgcaatgtg gcatacccgc catagctggc cccatagatc acgagcctgt
      601 cgggatcggc aattcctttg ctgataagcc atttggcgcc gtctgtaata tcatcctgca
      661 tggtcctgcc ccactgtttg aaggaagctt cccagaattt cctaccgaaa cctgtcgatc
      721 ccctgaaatt catctggaat acagcataac cgcggttggc caggaactgt acttcggggt
      781 tgaaacccca gttatcgcgt gcccaggggc ctccgtgggg gttcaggatc accgggagct
      841 ttttggcggt ctccattgtg tagcctttgg gcaaagtgag ataaccctgg attgtcaggc
      901 catcgcgcga ctggtacctg accggcagca tttcggccat ttcgttctca tcgatccagg
      961 ggctgacatc ggtgatattc gtcagtttat ccgcttcgcg gtcaaatatg taatacgccc
     1021 ccagtgattt gtcgctgtag gtgcggacaa tcaatttatt ttcatcatcc gtaatagcag
     1081 atattcccaa ttcatattta cccagatctt tgttaagacg ttcaaaaagc aaccgggtct
     1141 cttcgtcgaa gaagtgcctg ttccgcttat ctccgtaata attcgcagca gtcaacactt
     1201 ttctcttcct ggaaaagaag atgttgtcga tatcgtaatc gggattttca tagaggacct
     1261 ccacctcttt gccgttctta atatcgaaaa ccactgccgc cgctttatcc cggcccaggt
     1321 tggaaatagc atacacgttc ttattgtcaa acgtaaagaa tacgggcgac aaggtctctt
     1381 tgaaattggt tgtcagcacc ggtacaaact catccttctc ggtctcccgg taaaggatct
     1441 gtgagttgat cccgtcgaca atagctgtgg cagccctgag ctttccgtcg tgatcaaaga
     1501 tccagccctg gatattgccc gggttctcgg ccagcatttc catttctccc gtcctgatgt
     1561 tcagccggta aggatcaaat acctggggat tccgtttatt catgccaatg atgacatgat
     1621 cggggaattc gggcagatcg tcgataatat cggtcctgac gccttcaaaa tcggtgaaac
     1681 atttcagatt actgccatcg gcattcacac cgtacaaacg gaaattctca tcgcctccca
     1741 tatctttcag gtaaaggatc tgctcatcat tcggccagaa atagcccatg atatcgcggt
     1801 cgatctccga tgtaagccgt atggcggaat cggtcccgat tttctggacg aaaatattca
     1861 tcctgtcctg gtaaggagcc ttaaacgaaa aataggtacc tcccggtgaa atctggtaag
     1921 aagacttctc cggattctta aagaaatctt ctaaaggaat ctgttttgct ttcataatta
     1981 caggttgttg ctgacaagcg gccagcagcg tgatcaggat ggccggcaaa gcaaccatca
     2041 tcattaaacg tatgtatttc atattttgta gatttatttt gcgcaaaagt agaagtataa
     2101 agccaatccc gacacaagga atttcccttt ggtcattccc ggttaataaa aggaaaggtt
     2161 tcaggtttat tgcctgatga atttagctgt cagggttaga tcttcctgtt cattccatat
     2221 tctgagcaga aaaagccctg gcgccaggtt ttggatatcg atgctgttgc ttgtcatagc
     2281 gccttccata gccactgacc cgttcagttt aaacaccgtg taattcattc cggtcaacgg
     2341 tttggcgctg tggatctgaa tcttcgtacg agccggattc ggacttaaaa caatattggc
     2401 caggctatta ttaatcgccg gggtgccggt tatcaaccct gtttccaggc tggaggcgta
     2461 attacggaat ccttcccagt ggctcagcac atgcgggccg aaggccagat cctcggtaaa
     2521 catgcccgaa aggatcattt tgccgttttc aatcatttcg atggattgga ggtttgcccg
     2581 ggcgcccgaa atctgctctc cccaaacagc tgctcctccg gatgagtaac ttacaataaa
     2641 aatctcccgt gaaccgcagc agagggtata ttgcagttta agctggtcga atcgtatcgt
     2701 atcgaggtaa tgcccggcga tatagacatt cccgtaaggg tcggtatcga ggccgacgac
     2761 ctggtcgctg gccgtgctga acccatgccg ggcccacagc aattccatat cctggccgaa
     2821 tgcagcggta aaaatatcct ggttgaattc ccctgcatcg aaggtcttat tgccgaaaaa
     2881 taatttctca ctgaaattgc ctgcgacgta gaactgtccc tccaggccgg ctctgatcag
     2941 gttttcggtg gtaaggtagg tgccgttcaa ctggaaaacg cggtcgagcc cgccatcggg
     3001 gaaataccgg gcaatggcta ctcccacggg gtttggcgta tgcagggtcg tgtctccgaa
     3061 tgtgatatcg taataaaaag atgcagccag cagaatattc ccttcggagt cgatcgtcac
     3121 cgatgaagcc tggtcgctgg aagaaccgcc ggctgtggtg acccaggccg gttgtccgtc
     3181 aggcgaaaat ttgatcacga atgcatcgga accgcgggcg gccgtaacgg tcgtatcgcc
     3241 cacgataaag gagtcgtaga aatggccggc caggaaaaga tttccaccgg aatcggttgc
     3301 caggccccgg atataatcgg ccattccgcc acccagggtg tatgaccaga gataatttcc
     3361 ctccccgtcg aacctggcta tgaacagatc ctggcttccc tgcgaagtgt aaatatcgtt
     3421 gccaatctgg atggttccgt aataataacc ggtcattgta acattatcct cattatcgga
     3481 aacgatcttc cagacaaaat catcatcgtt gccgcccatc cgtatcgacc acaccggtgc
     3541 cccttccgga gtgtttttca ccagccagat atcggttccg cctgcagaga ccaggctttt
     3601 atttccgaat tgtaccgtgc cgagaaattc tccggccata taaaaatatc cgttatccgc
     3661 tccggccagt gttgtctttg catcggttgt attggatgga tttccgcact ggctggccca
     3721 ttcaaactcc cggggctggg cggtggagtg aaaatttatc agaaatgaga ttaagatcag
     3781 gagtataatt cgcatcacaa ttttttttac aaatatagaa atttaaatat gacagccggt
     3841 tcggtgccgt aaacgtattc ttcaacggca aaccgctggc cccatttctc tatcatattg
     3901 tcttaagaat ctagttatca gtcgggtcgt cgggaaattt gacccagtgg gcataatcct
     3961 gtccgagccg gcgcagggag cggctccaga gcattgaagg cgtggttcgg aggatatcgt
     4021 cagccgtcag gttcgtgacg acccaggagt tccgctccag ctcttcgtcg agctggttgg
     4081 gtgaccagcc ggaataaccg atgaagaaac gtatctgaga tggttttatc agcgaaagcc
     4141 cgatcatctc cttgaccctt tcaatatctc caccccagta tatacccggg attatttcga
     4201 tgctttcttc gatctcttcg cccatggtgt ggatgaaaaa aacgctgtcg ctctgaaccg
     4261 gccccccgag gaaaatcctg gaattgaact cgggaaagcc tttgaccaca tcattaaacc
     4321 tcgccttaac cggtttgttc acgatcaggc caaaagtccc ttcgtcgttg tgttcagcca
     4381 ggagtatgac cgatctcttg aagaaataat caagcaggaa cggctcggag atcaggatct
     4441 ttccccgggc cggtttaata ttgttggttt ttattttcag aaggtcatga atatctttca
     4501 tcgcctcaaa tctgcacggg tttgcccgtt ttgtttactt ttgaaatcta aattacaaaa
     4561 ataataccag ccttatgcaa cggtttgata acttttcaac gtatttgaga atgagggaag
     4621 aattcccggt tttcacctat gaaagttatc attatgaatt ttccggagag acccttgcgt
     4681 taaaattcac ttttaacctc tccggcaaat atttttttca tccgctggtg gtcatccctg
     4741 taaaaaactt cttccggcct caaaatgcat ttaaggccgg ggaactcgat gcactagtgt
     4801 tccacgccgg catgatcgag cttatttctt actggaaagc ggcctgcccg gccacggtgt
     4861 tcatcaagcc gcacagtctg accggtgagc aggcggcctt ctggaagaag ctttattatc
     4921 atggattagg tgaattcttc tatattaatt cgatccccgc agatgaagga agtttcatga
     4981 atcttcacgc aacgggaccg aatccgctaa tgccctttga ttattccggc accggacgca
     5041 tggtaccggt gggtggcggg aaagattcgg cggtcacgat tggattgatg gaaaaggccg
     5101 gcctggattg gttgcctttc gccctcagcc cggtatcagc caccggcgaa gtgatccgga
     5161 ccaccggcaa gacccggaac gacacacttg tttttgaacg gattatcgat ccaatgctca
     5221 tcgaactgaa taaccgcgga tttctcaacg ggcacacgcc tttttcggcc cttcttgcat
     5281 tttattccct tctggccgct tatcttaccg gtaaaaaaga cattgtcctt tctaacgaat
     5341 cgagcgcaaa tgagcccacc gtgccgggta cggggatcaa tcaccagtat tccaaatcgt
     5401 tcggctttga aaaggatttc cgggaatacg ttttgaagaa tatttcatcc ggtttcaatt
     5461 atttcagcct cctccggccg ctttcggaat tgcagattgc ccgccttttt tcggggatgc
     5521 caaagttttt tccgcatttc aaaagctgca acgcggggag caaagcaggc atctggtgcg
     5581 gccggtgccc gaaatgcctg ttcactttca tcatcctgtc gcccttcctg gagcctggcg
     5641 aactgacgcg gattttcggc aagaaccttc tcaacgacgc ttccctggcc ggttacctgg
     5701 atgagctgac cggcgaaagc cccgttaaac ctttcgaatg tatcggaact gttgacgaag
     5761 tgaataccgc attggcgatg acagttaaac attacgagcc ggaagctatg ccttatttgc
     5821 tgggaatatc ttccggaaga ataaataaag cagattctga cctttttgtt gacattgaaa
     5881 aacaaaagat gttgaaagat gaacactttg tacctgtgga atattcgaat ttgctggagg
     5941 aggtgctgag atgaaggaat tgttcgggtc gatggcatca ggtcgaaaaa ttcttatcct
     6001 gggattcgga aaggaggggc gatccacctg gcggttcatc agacagaatt accctgaaat
     6061 gacggttggt atagccgaca gggatgcata tctggctcaa aaaattgaag atcaggcaga
     6121 actggccggt atcgaccttc acctgggtga aaaatacctg gatgcgctga atgattactc
     6181 acttgtgatt aagagcccgg gagtcggatt gccggaaaat ctcagcctcc ggccggggat
     6241 cgtgatcacc tcccagaccc ggctgatgct ggaagcctac caccgccaga ttatcggcat
     6301 caccggcacc aaagggaaaa gcaccacttc cagcctggtg caccacctgc tggcaaatgc
     6361 gggcgtatcc tcggtgctgg tgggtaatat cgggcttccg ccatttgatt acctcgaccg
     6421 gatcgggccg gacactaaag tggtttatga aatgtcgtcg caccagttgg aagacattgg
     6481 tatagcccct catattgccg tcctgctgaa tctctttccg gagcatctcg ac
//
LOCUS       DAOPNH010000194         6532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13501_length_6532_cov_5.711749, whole genome shotgun sequence.
ACCESSION   DAOPNH010000194 DAOPNH010000000
VERSION     DAOPNH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13501_length_6532_cov_5.711749"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1000)
                     /locus_tag="PKZ74_10255"
     CDS             complement(5..1000)
                     /locus_tag="PKZ74_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93181.1"
                     /translation="MKKTIRNTVFFLLTAILPAAAFAQYNGAVLRLEKNSIRIGDQIR
                     AELSVTLPAGSQVKWPMLYDTLTAHVEILRKTDIDTVESNKTRFTLKQEFTVTSFDSG
                     YYRINPILVRYNQPGDTVTFFTETSPATLEVQTIQVDPAQDIKPIKPPLRAPVTFREI
                     WPWILLALGVIAIAIAIWYILKRRKNRPKPVATTRLDATKPPYEAAIDALENLKNKRL
                     WQAGMVKEYYSELTDIVREYIELRFMVRALEMTTAEISDAMRMTDAAVSTRDKLIQTL
                     TLADLVKFAKEQPLPVDNELSLNRCFEFVRETKPPKDAESLNAQPADSVEPKQLA"
     gene            complement(1002..1877)
                     /locus_tag="PKZ74_10260"
     CDS             complement(1002..1877)
                     /locus_tag="PKZ74_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPM93182.1"
                     /translation="METAEILKKVRKIEIKTRGLSHQVFSGHYHSAFKGKGMAFSEVR
                     EYQYGDDIRSIDWNVTARFNHPFVKIFDEERELTVMLVIDVSGSNEFGTKAQLKEELM
                     TEIAAVLAFSAIQNNDKVGVIFFSDKVEKFIPPKKGKTHILRIIRELIDFHPQSQKTN
                     ISEGLRYLSNALKKKCIAFVISDFMDHDFEQSLRIASRKHDIVAVRVVDQRESELPNV
                     GMIKVFNKESGKTMWIDTNDPGLRSNYRSNWQRTEQSLGQMFKRLGIDTATIATGENY
                     VKPLVNLFKVRESRF"
     gene            complement(2008..3000)
                     /locus_tag="PKZ74_10265"
     CDS             complement(2008..3000)
                     /locus_tag="PKZ74_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM93183.1"
                     /translation="MMETDIKALNDRIQQESAFIDMINMEMGKVIVGQKHMVERLLIG
                     LLSNGHILLEGVPGLAKTLAIKSLANVIDAHFSRIQFTPDLLPADLIGTMIYSQKKEE
                     FVVRKGPIFANFILADEINRSPAKVQSALLEAMQERQVTIGEHTFPLQEPFMVLATQN
                     PIEQEGTYPLPEAQVDRFMLKVVIGYPDKEEEKRILRQNISGINPATNTIIKPADISK
                     ARAVVRDVYLDEKIENYITDIVFSSRFPAEYKLRRFEPLIQYGASPRASINLALAAKA
                     FAFIKRRGYVIPEDIRAVAHDVMRHRIGLTYEAEAENITPEDIINEILNTVEVP"
     gene            complement(3270..4118)
                     /locus_tag="PKZ74_10270"
     CDS             complement(3270..4118)
                     /locus_tag="PKZ74_10270"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPM93184.1"
                     /translation="MQSGTETIKSVITAYLFKHLDHFRINLDHAIEEFDPEAIHQYRV
                     SVKRIRALVRIMNRLYDVPLFPDEFILPLRLMFKAGGTIRDDQVQLDLVEKIEKENVS
                     YYPLIKEYYRRRIEERKNSFYLKTFDFDQDSINRIRSQVEETLAPLDETFLEQGIYNR
                     LHNSMEKLKRKRYDLDQPEMLHRFRTRFKENGYVAEMFHFSEFTNRVTKSRYNRMKHF
                     GQELGNWHDFYQLRVKTALIFQECRDEALLDESIALHKYITPLHDKLFQEILHLIKRD
                     DSLFAI"
     gene            complement(4291..4839)
                     /locus_tag="PKZ74_10275"
     CDS             complement(4291..4839)
                     /locus_tag="PKZ74_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM93185.1"
                     /translation="MNNRQVSIPDELIMNKILIVRGVKVMIDSDLAELYGVTTKRLNE
                     QVKRNIKRFPEDFMFRLTEREKQEVVANCDHLERLKFSPNLPYAFTEHGAVMLASVLN
                     SDRAIIVNIQIVRVFNRMRQLLETHTDILRKLELLQKKDVEQDRQILLIFEYLKQLEQ
                     DRQQQLEQKKRKRIGFKRDSEA"
     gene            complement(5423..5962)
                     /locus_tag="PKZ74_10280"
     CDS             complement(5423..5962)
                     /locus_tag="PKZ74_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HPM93186.1"
                     /translation="MNNEEEKYKENFSITLNALFDKKEWVKAKELLESEINKFPNEYF
                     LYTSLAKVYFNLKQYQKASEAALMAVSIEPNDPLVVYDYACTLYSLDKYNEAINEWFK
                     IVNLGVKGILHGEYNEGIKWTKSIVNDSRFRIAMCYFEMGNKEIARKFVDDHLKHRQR
                     GIYSDFTKRQILKKQHELS"
     gene            complement(5959..>6532)
                     /locus_tag="PKZ74_10285"
     CDS             complement(5959..>6532)
                     /locus_tag="PKZ74_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93187.1"
                     /translation="VPDPLAEKHFEYTPYAYAFNNPITYIDPIGLDTVAATTAQPIKQ
                     DDVVVMEDGTEYTMNADETEIKPDDENNDDQGNDDQQNDDENNSSAGKVMAGTLLISG
                     ILLADDATVVGVFDDVAIPALLVAGSITAGAIWLHDQFAEHTSNARKSTWNKHSKKDP
                     GEITGKNRNTNRGDKNKKFEKKPNPNKRTK"
BASE COUNT         1609 a         1486 c         1467 g         1970 t
ORIGIN      
        1 aacatcacgc aagctgtttt ggttcaacac tatctgcagg ttgagcgttc aggctttcag
       61 catcctttgg cggtttggtc tccctgacga attcgaaaca acggttcagg ctgagttcat
      121 tatcgacagg cagcggctgt tctttggcga atttgacgag gtcggccagt gtaagggtct
      181 ggatgagctt gtcgcgtgta gaaaccgccg catcggtcat cctcatggca tccgaaattt
      241 ctgcagtggt catttcaagg gcgcggacca tgaaccgtaa ttcaatatat tccctcacaa
      301 tatcggtgag ttccgaatag tattccttca ccatgcccgc ctgccataac cgtttgttct
      361 tcaggttctc aagcgcatca attgcggctt cataaggcgg tttggtggca tcaaggcggg
      421 tggtggccac aggtttcggc ctgttttttc tccttttgag gatataccat attgcgatgg
      481 caatcgctat cacacccaat gccaggagga tccagggcca aatctcccgg aaagtgaccg
      541 gagcccgcaa cgggggtttg atcggcttga tatcctgcgc cggatcaacc tggatggtct
      601 gtacctccaa ggttgccggt gatgtttcgg taaagaaggt cacggtgtcg cccggctggt
      661 tgtagcggac caaaatcggg ttgatccggt aatatcccga atcaaaggag gttaccgtaa
      721 attcctgttt cagtgtaaac cgcgtcttgt tcgattccac cgtatcgatg tcggtcttgc
      781 gcaaaatctc gacatgggcg gtaagggtat catacagcat gggccacttc acctggctgc
      841 ctgccggcag ggtcaccgag agctcggccc ggatctggtc accgatccgg atcgaatttt
      901 tttccagccg caaaaccgcg ccgttgtact gtgcgaaggc cgctgccggc aggattgccg
      961 tcagtaaaaa gaaaaccgta tttctgatcg tttttttcat tctaaaacct cgattccctg
     1021 accttgaaaa gattaaccag cggtttgacg tagttttcgc ctgtggcgat cgttgcggta
     1081 tcgatcccca gccgtttgaa catttgcccg agagattgtt cagtccgttg ccagttcgag
     1141 cggtagttgc ttctcagtcc gggatcgttc gtatcgatcc acatcgtttt acccgattcc
     1201 ttattaaaga ctttgatcat gccgacattc ggaagctccg attcccgctg gtccactacc
     1261 ctcacggcca ctatatcgtg cttccggctg gcaatgcgga gcgattgctc gaaatcatgg
     1321 tccatgaaat ccgaaattac aaaggcaatg catttctttt tcaatgcatt ggagaggtat
     1381 cgcagccctt ccgaaatatt cgttttctgg ctttgcgggt gaaagtcgat cagttcccgg
     1441 atgatcctga gaatatgtgt ttttcctttt ttcggaggaa taaacttttc gaccttgtca
     1501 ctgaagaaga taacccccac tttgtcgttg ttctggatgg ctgagaaggc cagaaccgct
     1561 gcaatttcgg tcatcagctc ttctttgagc tgcgcttttg tgccaaactc gttcgatccg
     1621 ctcacatcga tcacgagcat gacggtcagc tctctttcct catcgaaaat cttgacgaaa
     1681 ggatggttga accgtgccgt gacattccag tcgatactcc ggatatcatc accatactgg
     1741 tattccctca cttcgctgaa tgccatccct tttcccttaa aagcggaatg gtagtgaccc
     1801 gagaacacct ggtgcgacaa cccgcgtgtc ttgatctcta tctttcttac ttttttaagg
     1861 atttctgcag tttccatatt aaaagaaata ttctattgtt ctattgttca attgatattt
     1921 aggtttttag aacttgacaa acatttaata agttgaataa atatatcttc taacactaga
     1981 acaattgaac aaatgaacag aatagcatta cggtacctct accgtattaa gtatctcatt
     2041 gataatgtct tccggagtga tattttcggc ttccgcttca taggtgaggc caatccggtg
     2101 gcgcatgaca tcgtgggcca cggcgcggat atcttccggg atgacatatc ctctgcgttt
     2161 gatgaaggca aaggctttgg cggccagcgc caggttgatg cttgccctgg gggaggcgcc
     2221 atactggatc agcggctcga agcggcgcag cttgtattcg gcagggaacc gggatgaaaa
     2281 tacgatatcg gtaatataat tttcgatctt ttcatccagg taaacatccc tgaccacggc
     2341 cctggccttg ctgatgtcgg caggcttgat tatggtattc gtcgccgggt ttatgcccga
     2401 tatgttttgc ctgaggattc gtttttcttc ttccttgtcg ggataaccga tcaccacttt
     2461 gagcataaag cggtcgacct gtgcttcggg aaggggatag gtcccttcct gctcgatcgg
     2521 gttctgggtg gccagcacca tgaatggctc ctggagcgga aaagtgtgct caccgatggt
     2581 gacctgcctc tcctgcatgg cttccagcag tgcgctctga acctttgcgg gcgaccggtt
     2641 gatctcatca gccaggataa agttggcaaa aatgggccct ttcctgacaa caaattcttc
     2701 ttttttctgg ctgtagatca tggtaccgat caggtcagcg ggtaaaagat caggggtgaa
     2761 ctggatacgg ctgaaatgcg catcgatcac attggccagc gatttgatcg ccagcgtctt
     2821 ggccaggccg ggaacccctt ccaggagaat gtggccgttt gaaagcagcc cgatcagcag
     2881 ccgttccacc atgtgtttct gacctacgat cactttgccc atctccatgt tgatcatgtc
     2941 gatgaatgcg ctctcctgct ggattcggtc gtttaaagcc ttgatatctg tttccatcat
     3001 aaaatgaatt aagcggcaca aaagtaaagc ttgcccgtat cagacaccta agggtttatg
     3061 ttaaaagttg ttaaagggct tgttaaagaa tgttaagcac tacctgtaag aggttgtctc
     3121 aaaagtcggg attgacttca aaatatcttc gtgtagcttc gtgatttctt cgtggaactc
     3181 tgtgtaacaa tcatttatta attacacgaa gattcacgaa ggtttcacga agatccacga
     3241 aggacttttg atacatcctc actgcaaatt cagattgcaa acagactgtc gtcccgcttg
     3301 atgagatgca ggatctcctg gaaaagcttg tcgtgcaacg gggtgatata tttgtgcaac
     3361 gcaatggatt catccagcaa cgcctcatcg cggcactcct ggaagatcag ggcggttttg
     3421 acccgcagct ggtaaaaatc atgccagttc cccagctcct gcccgaaatg tttcatccgg
     3481 ttgtaccgtg atttggtgac ccggttggta aattccgaaa aatggaacat ctcggccaca
     3541 taaccgttct ctttgaaacg ggtgcggaat ctgtgcagca tctccggctg gtccaggtcg
     3601 taccttttcc gcttcagttt ttccatcgaa ttatgaagcc tgttgtagat gccttgttcc
     3661 aggaaggttt cgtcaagtgg agcaagggtc tcttccacct ggctgcggat cctgttaatg
     3721 gaatcctgat cgaaatcaaa agtcttcagg taaaaggaat tcttccgctc ctcgatgcgc
     3781 ctgcggtaat actctttgat aagggggtag taactgacgt tttctttttc gattttttca
     3841 accaggtcca attgcacctg gtcatcgcgg atggtcccgc ccgctttgaa catcaaccgg
     3901 agcggaagga tgaattcgtc cgggaacaga ggcacgtcat ataacctgtt cataattctg
     3961 accagcgccc ttatcctctt aaccgagacg cggtactggt ggatcgcttc cggatcgaac
     4021 tcctcgatgg catgatcaag atttatcctg aaatggtcca ggtgtttgaa gagataagct
     4081 gtgatgaccg attttatggt ttcagtgccg gattgcatgg atttttgata atgccgttta
     4141 tgatgacggc ataaatgtat aaaagtccat tcaatctttt gttaagaaat gattaataaa
     4201 acaggaatgg ttcgtgttat tttattttag cacagggggg ctgagtcgca ggatgtttaa
     4261 gaaagaagtg agcatcattt tgttggagta ttaggcttca ctatcccgct tgaagccaat
     4321 gcgttttctt tttttttgtt ccagttgctg ctgccggtcc tgttccagtt gtttgaggta
     4381 ttcgaagatc agcaggatct gccgatcctg ctcaacatct ttcttttgta atagttccag
     4441 ttttcgtagt atatccgtat gggtttccag caactggcgc atcctgttaa atacccttac
     4501 gatctggatg ttgacgataa tggccctgtc gctgttcaag acactggcaa gcataacggc
     4561 tccgtgttct gtaaatgcat agggaaggtt tggagagaac ttaagccgtt cgaggtggtc
     4621 gcaatttgcg accacctctt gcttttctcg ctctgttagc cggaacataa aatcttcagg
     4681 aaagcgcttg atgttacgtt tgacctgctc attaagtctt ttggtagtca caccgtaaag
     4741 ctctgccaga tcactgtcga tcattacttt tacccctctt acgatcagga ttttgttcat
     4801 gatcagctca tcagggatgc tcacctgtct gttgttcata cattggttat tgacgttcaa
     4861 aaatgggatt taatgcatat tctgatgtaa ttggaggaca caatttgcgt cctccatcac
     4921 accatattaa tccgtttttt tcgaaaaaaa tacccggccg gagaaaaata ttttagtaag
     4981 gggctggttt ataagggaaa cggggttggt tcattgagac tccatctcga ttcgggcaga
     5041 tggagtctcg cagaaatgag atggagtctc gctgaaatga gatggagtct cattgaaatt
     5101 gagtctcgtt tccacccgcc tgaaaaccgg atgtagccgc aaggaggatc cgttaacgat
     5161 aaagcccaaa tcgcgatttc cgataaagaa ctgctgaacc agttcaagga agtggaaaaa
     5221 ctctcccagg aagacaaaca cctgatcaaa acctttatcg acgctttcct gaccaagagg
     5281 aaaattcagc aactggcggt gtaaatgaaa agaagctcat caaaatttac tgctcaagat
     5341 gtaacatgtc gtatttatac tctttattgg aaggatcaag caatcttaag tgggataaca
     5401 ttaatctgta aattattgcc gattaggata attcatgttg tttcttaagg atttgcctct
     5461 ttgtaaaatc actatatatt cctcgttgtc tatgttttaa atgatcatct acaaacttcc
     5521 tcgctatttc tttgtttccc atttcaaaat aacacatggc aatcctaaac cgggagtcat
     5581 taacaatcga ttttgtccac ttaataccct cgttgtattc accatgtaaa atacctttaa
     5641 ctcccagatt cacaatttta aaccattcat taatcgcttc attatattta tctaaggaat
     5701 acaaagtgca agcataatca tatacgacta aaggatcgtt tggctcaatt gaaactgcca
     5761 ttaaggctgc ttcagatgct ttttgatatt gtttaagatt aaaatatact ttagcaagtg
     5821 atgtataaag aaagtattca tttggaaact tattaatttc actttcaagc aattcctttg
     5881 cttttaccca ctccttctta tcaaataaag catttaaagt aattgaaaaa ttctctttgt
     5941 atttttcttc ttcattattc attttgtacg tttattaggg ttaggctttt tctcaaactt
     6001 tttattttta tctcctctat tagtatttcg attttttccg gttatctccc cagggtcttt
     6061 tttcgaatgt ttattccagg tcgattttct tgcattacta gtgtgttcag caaactgatc
     6121 gtgaagccat attgctcctg cagtaatact tccggcaacc aaaagggcag gaattgctac
     6181 atcatcaaac acacccacaa cagtagcatc atcggcaaga agtatacctg aaattaaaag
     6241 tgtcccagcc ataacttttc ccgcagagct attattttca tcatcatttt gctggtcatc
     6301 attgccctgg tcatcgttat tttcatcatc tggtttaatc tctgtttcat cagcattcat
     6361 cgtatattct gttccatctt ccatcaccac gacatcatct tgtttgattg gttgggcggt
     6421 agtagccgcg acagtatcta atccaatagg atcaatataa gttatgggat tgttgaaagc
     6481 atatgcatat ggcgtatatt caaaatgttt ctcggctaag ggatcaggca ca
//
LOCUS       DAOPNH010000195         6511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13567_length_6511_cov_6.457869, whole genome shotgun sequence.
ACCESSION   DAOPNH010000195 DAOPNH010000000
VERSION     DAOPNH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13567_length_6511_cov_6.457869"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKZ74_10290"
     CDS             <1..252
                     /locus_tag="PKZ74_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM93188.1"
                     /translation="VFGWISKLISLNIRQRLRRQGKLKEADDDNLMIPADHSAAIAMA
                     LFLYNEVHDEESNILTIERVSRIYSPWSSKYYNMRKPVR"
     gene            277..699
                     /locus_tag="PKZ74_10295"
     CDS             277..699
                     /locus_tag="PKZ74_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPM93189.1"
                     /translation="MRTFKFKIKGQEYEVEIKKLDNNIAEVEVNGSLYQVEVSREPKQ
                     SKTPILVRSEPSVPKSAHKFKKKISGTIEVRSPLPGNIMQVFVREADEVKKGDKLIVY
                     EAMKMENTIYAEKDGIVAKVKVKPGDTILEGDVLMEIG"
     gene            704..1885
                     /locus_tag="PKZ74_10300"
     CDS             704..1885
                     /locus_tag="PKZ74_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPM93190.1"
                     /translation="MNALDGIYRFLEYSGFANATPGHLIMILVGLIFIFLAIKFEYEP
                     LLLIPIGTGILIGNIPFFQSDSISFQIGIYEQGSVLNYLYYGVLKGIYPPLIFLGIGA
                     MTDFSSLISNPKLILLGAAAQIGIFVTFIGALLIGFNLPEAAAIGIIGGADGPTAIFL
                     SSRLANGGIVNGLQTQNLIGPIAIAAYSYMALVPVIQPPVMRLLTSKKERLIRMKPPR
                     AVSRLEKIMFPIVGLLLTTFISPGALPLLGMLFFGNLLKESGVTRRLADTAKSALIDI
                     VTIILGLTVGASTQADVFLTPKSIMIFGLGALSFIIASAGGVLFAKFLNLFLKPENKI
                     NPLIGSAGVSAVPDSARVSEMEGLRMDKTNHLLMHAMAPNVAGVIGSAVAAGILMSFL
                     M"
     gene            1980..2786
                     /locus_tag="PKZ74_10305"
     CDS             1980..2786
                     /locus_tag="PKZ74_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPM93191.1"
                     /translation="MKRFAGFLWIMVMILPFFLSSCNDDNDGINIFTVEDDKQLGLQM
                     EQQIQSSPAEFPLLQPGQYPDAYQHIQQVRDRILQSGLVDFDTEFNWDVHIINDDSVL
                     NAFCTPGGYIYFYTGLIKYLDNDAEFAGVMGHEMAHAAKRHSTEQMTKAYGLQVLLSV
                     VLGENPNVLAQMAAELAAGVASLAFSRDDEYESDEYSVKYLYETTYDARGVAGFFEKI
                     GGAGQPPEFLSTHPSPDNRVEKIYEIWQSLGGKEGETYPEEYQAFKNSLP"
     gene            2933..4459
                     /locus_tag="PKZ74_10310"
     CDS             2933..4459
                     /locus_tag="PKZ74_10310"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPM93192.1"
                     /translation="MPNNEELFKKIISHAKEYGFVFPSSEIYDGLSAVYDYGQYGVEL
                     KNNIRQYWWKSMVQHHENIVGIDAAIFMHPTTWKASGHVDAFSDPLIDNKDSKKRYRA
                     DVLVEEFMAKQQGKIDQEVEKARKRFGENFDEKLYLETNPRILEYARKIEEARSRMVK
                     VMEENDLAGFRQLILDLEIADPVSGTRNWTDVRQFNLMFSTQMGSVAEDASVIYLRPE
                     TAQGIFVNFLNVMKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVQP
                     GEEMKWFNYWKEQRMNWHLGMGIPKEKYRFHEHDKLAHYANAAFDIEFDFPIGFKELE
                     GIHSRTDFDLSSHQQYSGKKLQYFDTEQNQGFVPYVIETSIGLDRTFLAVLSYSYHEE
                     KLEDGTERVVLNIPPFLAPVKAAVLPLVKKDGLPEKALEIFDSLKYDYMCQYDEKDSI
                     GRRYRRQDAIGTPYCITVDHQTLEDNTVTIRERDSMKQDRIDIGGIAGIIANRLKGKN
                     "
     gene            complement(4539..5090)
                     /gene="pth"
                     /locus_tag="PKZ74_10315"
     CDS             complement(4539..5090)
                     /gene="pth"
                     /locus_tag="PKZ74_10315"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPM93193.1"
                     /translation="MKYLIAGLGNIGDEYANTRHNIGFEVVTALARLSGVSFESGRYA
                     DVARLKIKNKNLVLIKPSTFMNLSGKAVRYWLEHEKIPLENLLVVVDDLALPQGKLRL
                     RLKGGPGGHNGLMSIIETLGTEEFARLRFGIGSDFAKGYQTEYVLGRWTSAEEEIMVP
                     KVAEAVEMIRAFVISRPSFNSPQ"
     gene            complement(5112..5696)
                     /locus_tag="PKZ74_10320"
     CDS             complement(5112..5696)
                     /locus_tag="PKZ74_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPM93194.1"
                     /translation="MKTVSMSGSLRESVGKKDAKLQRSEGRIPCVLYGGKDQIKFVAD
                     EKSFHEIIFSPESYFIKMTVDGKTYDCVLKDIQYHPVSDTILHADFIEFSMDHPLTMS
                     VPVRLTGNAPGVIKGGQLVKKFRKLNVRALPADMPEAVTIDIGNLDIEQKITIREIAQ
                     EKFKILENPDRYIVAIRATRASATPDGKGEAPAK"
     gene            complement(5807..>6511)
                     /locus_tag="PKZ74_10325"
     CDS             complement(5807..>6511)
                     /locus_tag="PKZ74_10325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPM93195.1"
                     /translation="KNIVSVIPYFGLARQNRKDRPRVSIASKLVANLLTAAGVQRIVT
                     LDLHADQIQGFFDVPVDHLFASSIFRGYIESLKLPNLTMASPDTGGTRRAAAYAKALH
                     TDFVICYKQRSKPGQVETMALVGDVKGRDIILVDDIIDTGGSITKAAQLMMDKGANSV
                     RAFATHPVFSGSAIEKIENSVFTEVVVTDTIPLKEKSSKITVLTTADLFAEVIRRIEN
                     FKSINSLFIMDESDKK"
BASE COUNT         1613 a         1571 c         1734 g         1593 t
ORIGIN      
        1 gtatttggct ggatatcaaa gcttatcagc ctgaatatcc gtcagagact gagaaggcag
       61 gggaaactca aagaggctga cgacgataac ctgatgatcc cggccgacca cagcgcggcc
      121 atagccatgg ccctctttct ttataatgag gtgcatgatg aagagagcaa tattctgacg
      181 attgaacggg tttcccgaat ctattctccc tggagcagta aatattacaa tatgagaaaa
      241 ccggttcggt aaccggatga taaacactac cagaccatgc gaactttcaa gtttaagatc
      301 aaaggacagg aatacgaggt cgagatcaag aaactcgaca acaacatagc cgaggtagag
      361 gtgaacgggt ctctttacca ggtggaggtc agccgggagc caaaacagtc gaaaacaccg
      421 atactggtcc gctccgagcc ctccgtcccg aaaagcgccc ataagttcaa aaagaagatc
      481 agcggtacga ttgaggtcag atctccgctt ccgggaaata tcatgcaggt tttcgtcagg
      541 gaagccgatg aagtgaaaaa aggtgataaa ctgatcgttt atgaggcgat gaaaatggaa
      601 aacactattt atgccgaaaa ggatgggatt gttgccaaag taaaggtcaa acccggcgac
      661 actattctgg aaggggatgt tttaatggaa attggctgat aagatgaacg cacttgacgg
      721 aatttacagg tttttggaat actcaggatt tgccaatgcc acacccgggc acctgatcat
      781 gatcctagtg ggcttgattt ttattttcct ggcgataaaa tttgaatatg aacctttatt
      841 gctgatcccg atcggaacag gcattttgat aggaaatatc ccgttcttcc agtcggatag
      901 catttccttc cagataggca tctatgagca ggggagcgta ttgaattatc tctattacgg
      961 cgtattgaaa ggcatctatc cgccactgat cttcctgggg atcggcgcga tgaccgattt
     1021 ttcgtcgctg atctcgaatc cgaaactgat cctgctcggt gcagcggcgc agataggtat
     1081 tttcgtaacc tttatcggcg ccttactgat cggattcaat ctgccggagg cggccgctat
     1141 tggcatcatt ggtggcgccg acggcccgac ggctatcttt ctttcatccc ggctggccaa
     1201 cggcgggatt gtaaacggac ttcagacgca gaacctgatc ggcccgattg ccattgcagc
     1261 ctattcttac atggcgctcg ttccggtcat tcaaccgcct gtcatgcggc tgctgacttc
     1321 caaaaaagag cgtctgatca ggatgaagcc cccaagggca gtctcacgcc tggaaaagat
     1381 catgtttccc atcgtggggt tgctgctgac cacttttatt tcgccaggcg ccctgccgct
     1441 gctgggcatg ctcttcttcg gcaacttatt gaaagagagc ggcgtaacac gtcggcttgc
     1501 cgatactgcc aaatcggccc tgatcgacat cgtcaccatc atacttggcc ttaccgtggg
     1561 ggcctccaca caggccgacg tgttcctcac cccgaaatcg atcatgatct ttggcctggg
     1621 agccttatct ttcatcatcg catctgccgg cggagtcctt ttcgccaaat tcctcaacct
     1681 tttcctgaag ccggaaaata agatcaatcc actgatcgga tcggcaggtg tttcagccgt
     1741 gcccgacagt gcaagggttt cggagatgga agggctgagg atggataaaa cgaaccacct
     1801 gctcatgcat gccatggcgc cgaatgtcgc cggcgtcatc ggatcagccg tggccgcagg
     1861 aatcctgatg agcttcctga tgtgagttcc aggttccagg gttcaaaggt tacaggttta
     1921 tagaggttat actgacttct ttctccaata tttgcctaca tttgcagaaa aatttgatta
     1981 tgaaaagatt tgccggtttt ttatggatta tggtgatgat attaccattt ttcctgtcgt
     2041 cgtgcaatga cgacaatgac gggatcaata ttttcacggt cgaggacgac aaacagctcg
     2101 gcctgcagat ggagcagcag atacagagca gccccgccga attcccgctt ttgcagcctg
     2161 gtcagtatcc cgatgcgtac cagcacattc agcaggtcag ggataggatt ctgcagagcg
     2221 gcctggtcga ttttgataca gaatttaact gggatgtaca cattattaat gatgattcgg
     2281 tattgaatgc cttttgtacg ccgggcggat acatttattt ctatacgggc cttattaaat
     2341 acctggataa cgatgcggaa tttgccggcg tgatggggca tgagatggcc catgccgcca
     2401 aaaggcactc caccgagcag atgacaaaag catatggctt gcaggttctc ctttccgtgg
     2461 ttctgggtga aaatccgaac gtgcttgccc agatggccgc agaactggca gccggtgtgg
     2521 cttctcttgc cttcagccgc gatgatgaat acgagtcgga tgaatattcg gtgaagtatc
     2581 tgtacgaaac aacttatgat gcgcgcggag tggccggatt ctttgagaaa atcggcggag
     2641 cagggcaacc gcccgagttt ctgagtaccc atcccagccc cgataacagg gttgaaaaaa
     2701 tatatgagat atggcaatcg ctgggcggaa aagaggggga aacatacccg gaggagtacc
     2761 aggcgtttaa gaattcatta ccttagaagt aatttgcgtt taattgttcg actgttcaat
     2821 tgttattgtg gtaaggggca atcagagaga atattttata aattaccaag ccaataacaa
     2881 ttgaaccata gaacaattga gcaattgacc agtagcaata ataccctaaa tgatgccaaa
     2941 caacgaagaa ctctttaaaa agatcatctc ccatgccaag gaatacggct ttgtatttcc
     3001 ttccagtgaa atttatgacg gactgagcgc ggtttacgat tatggacaat atggcgtcga
     3061 actgaagaac aatatccgcc agtactggtg gaagtcgatg gtacagcatc atgagaacat
     3121 tgtcgggatc gatgcggcta tctttatgca cccgacaacc tggaaggcct ccgggcatgt
     3181 cgacgccttc agcgacccgc tgattgacaa caaagacagc aagaagcgct accgtgccga
     3241 cgtactcgta gaagaattca tggccaaaca gcagggcaag atcgaccagg aggtggagaa
     3301 agccaggaaa cggtttggag agaacttcga cgaaaagctt tacctggaga ccaatcccag
     3361 gattcttgaa tatgccagaa agatcgaaga ggcacgcagc cggatggtga aggtcatgga
     3421 agagaacgac ctggccgggt tccgccagct tatcctcgac ctggagatag ccgacccggt
     3481 ttcggggacg cgcaactgga ccgacgtgcg ccagttcaac ctgatgtttt ctacgcagat
     3541 gggttcggtg gctgaagacg ccagcgtgat ctatctccgt cccgaaacgg cgcaggggat
     3601 ctttgtgaat tttctgaatg tgatgaagac cggcaggatg aagattcctt tcggtattgc
     3661 acagaccggc aaggcattcc gcaatgagat cgtggcgcgg cagttcatct tccggatgcg
     3721 cgagttcgag cagatggaga tgcagttctt tgtgcagccc ggcgaagaga tgaaatggtt
     3781 caactactgg aaagagcagc ggatgaactg gcacctggga atgggcattc cgaaggagaa
     3841 gtaccgtttc catgagcacg acaagctggc gcactacgcc aatgccgctt tcgatatcga
     3901 gttcgacttc ccgatcggct ttaaggagct agaagggatc cactcccgca ccgatttcga
     3961 tctttcttcg caccagcaat attccggcaa aaagctccag tatttcgaca cagagcagaa
     4021 ccagggcttc gtaccgtacg tcatcgagac ctctatcggc ctcgaccgga cattcctggc
     4081 agtgctgagt tattcgtacc atgaagagaa gctagaagat ggcaccgagc gcgtcgtgct
     4141 gaacatcccg ccgttcctgg cgccggtcaa ggcagcggtg ctcccgttgg taaagaaaga
     4201 cggtttaccg gagaaagcgc tggaaatctt cgacagtttg aagtacgatt acatgtgcca
     4261 gtatgatgag aaggactcga tcgggcggcg ttaccggagg caggatgcca ttggcacgcc
     4321 ttactgcatc acggtcgacc accagacgct ggaggacaac accgtcacca tccgcgagcg
     4381 cgacagcatg aagcaggacc gcatcgatat cggcggaatt gcagggataa ttgctaatag
     4441 gttaaaaggg aaaaactgag tattttaacc acaatagaca catagaacac aataggtttc
     4501 acaatagatc tatgtgcttt ctattgtgcc ctatgtgact attgtggtga attaaaggat
     4561 ggccttgaaa taacgaatgc cctgatcatc tccaccgctt ccgccacctt cggcaccatg
     4621 atttcttcct cagccgaagt ccatctacct aaaacatatt ccgtctggta gcctttggcg
     4681 aaatcactcc cgataccgaa acgcaaccgg gcaaattctt ctgttccaag ggtttcgatg
     4741 atgctcatga ggccgttgtg gccaccgggg cctcctttta gccgtaatct cagtttgcct
     4801 tgcggaaggg cgagatcatc gaccaccacc agcaggtttt caaggggtat cttttcatgc
     4861 tcaagccagt agcggacggc cttgccgctc aggttcatga aggtggaagg cttgatcaga
     4921 acgaggtttt tgtttttgat cttcagacgg gcgacatcgg catagcgccc gctttcaaaa
     4981 gaaacacctg acagccgggc cagtgcagtt acgacttcaa aaccgatatt atgcctggtg
     5041 ttggcatatt cgtcgccgat attgcccaat cctgctatca ggtatttcat aattcagacc
     5101 cgttgaaggc cttattttgc cggggcttcg ccttttccgt cgggagtggc cgaagcgcgc
     5161 gtagcccgga tggccacgat ataccggtcg ggattttcga ggatcttgaa tttctcctgt
     5221 gctatttccc ggatggttat tttctgctcg atatccaggt ttccgatgtc gatggtcact
     5281 gcttcgggca tgtcggccgg aagagcgcga acgttcagtt ttctgaattt tttcaccagt
     5341 tgccccccct tgatcacacc gggtgcattt ccggtaagcc tgacaggaac gctcatcgtc
     5401 agcgggtggt ccatgctgaa ttcgatgaaa tcggcatgca ggatcgtgtc gctgaccggg
     5461 tggtactgaa tgtctttcag cacgcagtcg taggttttgc catcgacggt cattttgata
     5521 aaatacgatt cgggggagaa gatgatttcg tggaacgatt tttcatcggc cacaaacttt
     5581 atctggtctt ttccgccgta aagcacgcac gggatacgcc cctcggaacg ctggagctta
     5641 gcatcttttt tccctacgct ctccctcaga gagccgctca tagatactgt tttcatgaag
     5701 ataaattgtt aaattgttaa attgtaaaat tgttaaattg ttaaattgtt ctattgttct
     5761 attgctgact gcttactgcc gactgctgac tgccgactgc cgactgctac ttcttatcgc
     5821 tttcatccat aataaacaac gagttgatcg acttgaagtt ctcgatacgg cggatcactt
     5881 cggcgaagag gtcggctgtt gtcagaacgg tgatcttgct gcttttttcc ttcagcggga
     5941 tggtatcggt gacgacgact tcggtaaaga ccgaattttc gatcttttcg atagccgagc
     6001 cggagaaaac cgggtgggta gcgaaagcgc ggacgctgtt tgctcccttg tccatcatga
     6061 gttgggctgc tttggtgatc gagccgccgg tatcgatgat gtcatcgacc aggatgatat
     6121 cgcgtccttt cacatccccg acgagagcca tcgtttcgac ctgtcccggc ttggagcgct
     6181 gtttgtagca gatcacgaag tcggtgtgca gcgctttggc ataagcggcg gcgcggcgtg
     6241 tcccgccggt gtcgggcgag gccatggtca ggttcgggag cttcagcgat tcgatataac
     6301 cgcggaagat agacgaagca aagagatggt cgaccggcac gtcgaagaat ccctggatct
     6361 ggtcggcgtg gaggtcgagc gtaacgatac gctggacgcc ggcggctgtg agcaggttgg
     6421 caaccagttt ggaggcgatg gagacgcggg ggcggtcctt tcggttctgc cgggccagcc
     6481 cgaagtaagg gatgacggac acaatatttt t
//
LOCUS       DAOPNH010000196         6461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13748_length_6461_cov_7.039182, whole genome shotgun sequence.
ACCESSION   DAOPNH010000196 DAOPNH010000000
VERSION     DAOPNH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13748_length_6461_cov_7.039182"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..924
                     /locus_tag="PKZ74_10330"
     CDS             334..924
                     /locus_tag="PKZ74_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93196.1"
                     /translation="MKNRIFTSMTFMVAALISLAFITGGCKSKKETTAVQAPAPGEVE
                     VSVYCSGPEYRSDNDYFRANSIGESADQATSQKKALSNAKANLAGFIETTLKATFDNY
                     VKDSEMNNVSETLEKYEGLSREVVNQKLNGIKVICEKQTRTTTNTYKTYVAIELAGDE
                     IASAMNQRLSTDDKLFIDYNYEKFKETFDKEMENMK"
     gene            1006..2046
                     /locus_tag="PKZ74_10335"
     CDS             1006..2046
                     /locus_tag="PKZ74_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93197.1"
                     /translation="MKIILATWITLFLIPVIVHSQVNVTNSVEVDYNYQQYKSVFDEE
                     QGAFGEPAPDPGTPPPILFNTVPGWLQEPMPASAASFQVIGISDPGLDSVTARRQAIF
                     RALYLGSLMNGCRVRNLVDYYSQEKSAHNSELTGKYADYFEFTSFSPHGVDETTVVNQ
                     HLNENGECLVIIDFPVYSGGNPALAFQTNGMITEFQKNGKYESNSRIDFSLKAGRHDD
                     ADGFRYLFRSINGLVDMESYSGNEMLPQADARYKYTQTVTTEAPRDKQGIKTDRGLWN
                     ALLLAIIKDITVDIRTRSALVKSTADNYTGNSLALDREAVTGKLSFRLNRIILYDINL
                     WAILEQINFTQP"
     gene            2043..4709
                     /locus_tag="PKZ74_10340"
     CDS             2043..4709
                     /locus_tag="PKZ74_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93198.1"
                     /translation="MKPRTNKFLISLILSCIIILPFITKGQTFEEFKKQRSQEYKQYV
                     ENETKKMQELRDRYDAYVKAREQEFADYISKEWESYEAFKGRPVPVEPKPQVIPTYTP
                     PPQRTEEPAAKIPVKVKEIPRPVEQPAARINPVIEKIPPVDYLKSDLEFSFYGSKIML
                     DYDKALVIPPPSPVSEDGIGLYWTKACKANFNGLINQMLQYKSMLGLNDWGYYQLVRE
                     TSDKISMPDPNARNLLTWFLLTRSGYRAKISFAENKTSVIIPSNQTIYGRDYLTISQV
                     NYYFVDPIASNVIQTYARDYPDATRVFDFTIDRALNLGESPLVKNLSFTYNDQPHQVK
                     VTINQNVINFYKDYPLVDMNVYFDAALERLAAESIGEALLPVLTPMTEVDKVNCLLRF
                     VQTAFPYKVDQEQFGREKYFFAEELFFYPYSDCEDRSVLFSYLVSELLGMKVIGLGFD
                     GHVATAVHFSDKAGVNGAYFTFRNEKFIIADPTFINAPAGLAMPQYSASQAEIIEIAK
                     VNYAENTGVDFRELVRKAGGYRGGNQQDMIADENGNVYITGYYNETFQVGAKTLTGNV
                     DTRNAFAAKFNKYGELQWVAAPTGKAVTTGFGITQDEQQNVIVAGSFLGQAHFAGYTV
                     TSKQGKPDVFVAKYDPQGHCVWAGQASLDTIDQQSFLKYVAKFDRTGKHLATDLFLEN
                     VEFSQNGLFTDNDGTCYLAGNLSGTTGFRVPERTFMNGGEVDFPSMIKKESDKLISQK
                     VERNIAAIFAVTSLIRSNGMMIPGQAAQQALDKYQPLFRKQFPELYSMFGKITFMKNG
                     DGIVTLATKDHETIYYDKLKISDGTQIKIVQLQDGNERLDILSGIKVGKMVIWFSLNY
                     IQMDRLTGNLLFDYDSSHMQLNMNLKEDIIGD"
     gene            4727..5476
                     /locus_tag="PKZ74_10345"
     CDS             4727..5476
                     /locus_tag="PKZ74_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93199.1"
                     /translation="MKDDYKIRRLITAAGLFVLAVACLLTSASCKKLDFTRTEPIGQD
                     KWLHYDDGVNYDGIFANSGGNFDIAIRFSASELAPYDGFRISKLKFFPKVGYPAVYYI
                     TIWSGTEPPTLLKVQEISSVIAETWNEVSYDESYYIDASSDMWFGVWITDYPASTYPA
                     GCDNGPAVVGKGDLFSPDDGVTWYSLYTANSELNYNWNLQVYVTNLYAKTVPLAPSDE
                     SGLTKYKTSLRDLAPVEGVMNGVSSQKTDRP"
     gene            5473..6339
                     /locus_tag="PKZ74_10350"
     CDS             5473..6339
                     /locus_tag="PKZ74_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93200.1"
                     /translation="MKTKISALSFTILALLVSLFCSQGLNAQTKAKIENVDFFAESNR
                     IIITYDINGSGSGETFNIWILVKTASGKILSARTTSGDIGKGIQGGPGKRIEWSYSAD
                     NIVIEEDIGIEVLASLEAAAKKTEEVVQPVKEPEKEPAKEPQKKEEPVQQQYTGKKVS
                     MGAALAMSAVLPGLGKTYAKKKGAAYLWGVVGYGMVAGSVVLNHAAYKNLETYRESSA
                     ADRDDYYDKAKLQAIGSYVLIGSAAIIWVTEFISTGVNAGKAKKRQGSVRLNGGFDDR
                     FGVPVVGLTYKF"
BASE COUNT         1752 a         1705 c         1596 g         1408 t
ORIGIN      
        1 aaaaggtggt acactttgct caggaatcac tggtacactt tcgtcggaat attcaatttc
       61 cccccttttc ccttttccct gttccccaaa agctttcgtg catagtgcat ttgaaatatg
      121 acatgccatg cctcatgcca aatgccaaat gaaatgtaaa atgaaatgca aaatgcaaaa
      181 tgcaaaatgc aaaatgcgaa catcttcctg cccaaagaat aatttttctt ccgcttacag
      241 tctgtgttgc tatttttctt tgtaatttgg aggtttcaaa tcaaatcgat catttaacca
      301 tctaaatatt tattaaccag taatgcaaaa gtcatgaaaa acagaatctt tacctcaatg
      361 acctttatgg tcgcagcttt aatctccctg gcttttatca cgggaggatg caaatcgaaa
      421 aaagaaacga ccgcggtcca ggctcctgct ccgggcgagg tcgaagtcag cgtctattgc
      481 agcggaccgg aataccgcag cgataacgac tacttccgcg ccaacagcat cggcgaaagc
      541 gccgaccagg ctacttccca gaagaaagcc ctcagcaacg ccaaagccaa cctggccgga
      601 ttcatcgaaa ccaccctcaa agccaccttc gacaactatg tcaaggacag cgagatgaac
      661 aacgtcagcg aaaccctcga gaaatacgaa ggactcagcc gcgaagtcgt taaccagaag
      721 ctcaacggca tcaaggtgat ctgcgaaaag caaacccgca ccaccaccaa tacgtacaaa
      781 acctacgtag ccatcgagct ggccggcgac gagatcgcat ccgcgatgaa ccagcgcctc
      841 tccacggatg acaagctttt catcgactac aactatgaaa agttcaagga aactttcgac
      901 aaggaaatgg aaaatatgaa atagtaggag atcctcagga attcttatta ttcacgcagg
      961 ggcactgcgt cggaagaggc agggcccctg tttatctttc cggtcatgaa aattatcctg
     1021 gcaacgtgga ttactttatt cttaatccct gtgatcgttc attcgcaggt gaatgtgacg
     1081 aattcggtcg aagtcgacta taattaccag caatacaaat ctgtttttga cgaagaacag
     1141 ggggcgttcg gcgaaccggc acccgacccc ggcacgcctc ctcccatttt gttcaacacc
     1201 gtccccgggt ggctccagga accgatgccg gcttcagcag cctcgttcca ggtgatcggg
     1261 atctccgatc cgggcctcga ttcggttaca gcccgccggc aggccatctt ccgcgccctc
     1321 tatctcggat cgctcatgaa cggatgcagg gtaagaaacc tggtcgatta ttattcacag
     1381 gaaaaatcgg cgcataacag cgaacttacg ggcaagtatg ccgattattt cgagttcacc
     1441 agtttttcgc cgcatggcgt cgatgaaacc actgtcgtta atcagcatct caatgaaaac
     1501 ggggagtgcc tcgtgatcat cgactttccg gtttattcag gaggaaatcc cgcactggcc
     1561 tttcaaacca acggcatgat caccgaattc cagaaaaacg gtaaatacga gagcaattcc
     1621 aggatcgatt tttcgctgaa agcaggccgg catgatgatg cagacggttt ccggtatctc
     1681 ttccgctcaa ttaacggtct ggtggatatg gaatcctatt ccggaaatga aatgctgccg
     1741 caggccgacg cccggtacaa atacacccag accgtgacaa ccgaagctcc ccgcgacaaa
     1801 caggggatca aaaccgaccg cggcctgtgg aatgcgcttc ttttagccat cattaaagac
     1861 attacggtgg atatcaggac ccgctcggcc ctggttaaat ccaccgccga caactatacg
     1921 ggaaattcac tcgccctcga ccgggaagcg gttacaggca agctttcgtt ccggctgaac
     1981 cggatcatct tatacgatat taacctttgg gctattttgg aacaaataaa tttcacccag
     2041 ccatgaaacc aagaacaaat aaatttttaa tctccctgat cctttcgtgt atcataatcc
     2101 tgccgttcat cacgaaaggc cagacattcg aagagtttaa aaagcagcgc agccaggaat
     2161 acaagcagta tgtcgagaat gaaacgaaaa agatgcagga actgcgcgac cgctacgatg
     2221 cgtatgtaaa ggcaagggag caggaatttg ccgattacat cagcaaggag tgggaatcct
     2281 acgaagcgtt taaaggaagg ccggttccgg ttgagcccaa accccaggta atcccgacct
     2341 atacccctcc gccccaaagg acggaagagc cggccgccaa gatcccggta aaggtaaagg
     2401 agatcccgcg tccggtcgaa caacctgccg cccgcatcaa cccggtgatt gagaagatcc
     2461 cgccggtcga ttatcttaaa tccgacctcg aattctcctt ctacggctcg aaaatcatgc
     2521 tggattacga caaagcactg gtcattccgc ctccttcccc ggtgagcgaa gacgggatcg
     2581 gactatactg gaccaaggcc tgcaaagcca atttcaacgg cctgatcaac cagatgctgc
     2641 agtacaaaag catgctgggg ctgaacgact ggggctacta ccagctggtc agggagacct
     2701 cggacaagat cagcatgccc gatccgaatg cccgcaacct gctcacctgg ttcctgctga
     2761 cccgttcggg ttaccgggcc aagatatcct tcgccgaaaa caagaccagc gtgatcatcc
     2821 cttccaacca gaccatctac ggaagggatt acctcaccat cagccaggtg aattattatt
     2881 tcgtcgatcc gatcgccagc aacgtgatcc agacctatgc ccgtgattat ccggatgcta
     2941 cgcgcgtttt cgactttacg atcgaccggg cgctgaacct gggcgaatcg ccgctggtta
     3001 aaaacctgag ctttacctac aacgaccagc ctcaccaggt gaaagtcacc atcaaccaga
     3061 atgtgatcaa tttctacaag gattacccgc tggtcgatat gaacgtctat ttcgacgcgg
     3121 cgctcgaacg gctggccgcc gaatcgatcg gcgaagcgct cctgccggtc ctgacgccga
     3181 tgacggaggt cgataaggtc aactgcctgc tgcggttcgt gcaaacggcg tttccctata
     3241 aagtcgacca ggaacagttt ggccgcgaaa aatacttctt cgccgaggaa ctctttttct
     3301 atccctattc cgattgcgaa gaccggtcgg tacttttctc ctacctcgta agcgaactgc
     3361 tggggatgaa agtgatcggg ctcggatttg acgggcatgt ggctaccgcc gtgcatttca
     3421 gcgacaaggc cggggtgaat ggcgcctact ttacgttccg caacgaaaaa ttcatcatcg
     3481 ccgatcctac cttcatcaat gcgccggccg gcctggccat gccgcagtat tccgcttccc
     3541 aggctgagat catcgaaata gcaaaagtca attatgccga gaataccggg gtcgatttcc
     3601 gggaactggt ccgcaaagcc ggcgggtacc gcggtggcaa ccagcaggat atgatcgccg
     3661 atgaaaacgg gaatgtttat atcacgggat attacaacga aacattccag gtcggggcga
     3721 agacgctcac gggcaatgtt gatacccgga acgcttttgc agccaagttc aacaaatacg
     3781 gtgagctgca atgggtggcc gccccgacag ggaaagccgt tacgaccggt tttggcatca
     3841 cgcaggatga gcagcagaac gtgatcgtcg ccggatcgtt cctgggccag gcgcacttcg
     3901 ccggctatac cgttaccagc aaacagggaa aacccgatgt gttcgtcgcc aaatacgatc
     3961 cgcaggggca ttgcgtctgg gcaggccagg ccagcctcga caccatcgac cagcaatcgt
     4021 tcctgaaata tgtggctaag ttcgaccgga ccggcaaaca cctggccacc gatctcttcc
     4081 tggaaaatgt cgaattctcc cagaacgggc tgttcaccga taacgacggc acctgttacc
     4141 tcgccgggaa cctcagcgga accacgggat tccgggttcc cgaaaggaca ttcatgaacg
     4201 ggggagaggt cgattttcca tcaatgataa aaaaagagag tgacaagctc atctcccaga
     4261 aagtggaaag aaatattgcc gccatctttg ccgtaacaag cctgatccgc agcaacggga
     4321 tgatgatccc gggacaggcc gcccagcagg cgctcgataa ataccagcct ttgttcagga
     4381 aacagtttcc ggagctttat tccatgttcg gaaagatcac ctttatgaaa aacggtgacg
     4441 ggattgttac ccttgcgacc aaagaccacg aaacgatata ctacgacaaa ctcaagatct
     4501 ccgacggcac ccagatcaaa atcgtccagt tgcaggatgg caacgaacgc ctcgatatcc
     4561 tcagcgggat caaagtcggg aaaatggtca tctggttctc gttgaattac atccaaatgg
     4621 accggctgac gggaaacctg ctgttcgatt acgacagcag ccatatgcaa ctcaatatga
     4681 acctgaaaga ggacattatc ggagattaat cactttaaac cggattatga aagacgacta
     4741 taaaatacga cgactcatca cggctgcagg cctgttcgtt ctggcggttg catgccttct
     4801 gacatcggcc tcctgcaaaa agctcgattt tacccgaacc gagccgatag gtcaggataa
     4861 atggcttcat tatgacgacg gagtgaacta cgatggcatt tttgccaatt cgggcgggaa
     4921 tttcgatatc gctatccgct tcagcgcatc cgaactggct ccttacgatg gtttcaggat
     4981 ttcaaaactg aagttctttc cgaaagttgg ctatccggcg gtgtattaca tcacgatctg
     5041 gtctggaacc gaacctccta cactgttgaa ggtgcaggaa atcagcagtg taattgccga
     5101 aacctggaac gaagtcagct atgatgaatc ttattatata gatgcttctt cggatatgtg
     5161 gtttggcgtc tggatcaccg attacccggc ttctacctac cctgccggat gcgataacgg
     5221 cccggctgtt gtcggcaaag gcgacctctt ttcaccggat gacggcgtga catggtattc
     5281 gctctatacg gccaattcgg aactgaatta caactggaac ctgcaggttt atgttacaaa
     5341 cctgtatgcg aaaacagtgc cgctggcccc gtcggatgaa tccggcctga cgaagtataa
     5401 aacatccttg cgcgatctgg cgccggttga aggtgtcatg aacggtgtat catcacaaaa
     5461 aaccgaccgc ccatgaaaac aaaaataagc gccctcagct ttacgattct tgccctgctg
     5521 gtcagtttgt tctgcagcca gggcctgaat gcacagacaa aagcgaagat cgaaaatgtc
     5581 gatttcttcg ccgaaagcaa caggatcatc attacctacg acatcaacgg gagcggatcc
     5641 ggcgaaacct tcaacatctg gatactggtg aaaacagcat cgggaaagat cttgagcgca
     5701 cgaaccacct ccggcgatat cggcaaaggt attcagggcg gtccgggaaa acggatcgaa
     5761 tggagctatt ctgccgataa tattgtaatt gaggaagata tcggaatcga ggtgctcgct
     5821 tctctcgaag ccgctgccaa aaagacggaa gaagtggtac agcccgttaa ggaaccggaa
     5881 aaggagcctg cgaaagagcc gcagaagaaa gaagagcctg ttcaacagca atatacggga
     5941 aagaaagtct cgatgggcgc cgcgctggcc atgtcggcgg ttttgcccgg cctggggaaa
     6001 acgtatgcca agaaaaaggg tgcggcttac ctctggggtg tggtgggcta cggaatggtc
     6061 gccggctcgg ttgtattgaa ccacgctgct tataagaacc tggaaactta ccgcgaatca
     6121 agcgcggccg accgggatga ttattacgac aaggcaaagc tgcaagccat cgggtcttac
     6181 gtcctgatcg gcagcgcagc gatcatctgg gtgacggagt tcatttcaac cggcgtcaac
     6241 gcaggaaaag caaagaaaag acaaggcagt gtccggctga acggcggatt tgacgaccgt
     6301 ttcggcgtcc ccgtcgtcgg attgacatat aaattttgaa ctttgaactt tgaactttga
     6361 actttgaact ttgaattatt ttagcgtatg ttcaggacaa tattaatatt tgcagcactt
     6421 ctggcattcg tgggcctgaa tgcccaggtg atctcgggaa a
//
LOCUS       DAOPNH010000197         6459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13756_length_6459_cov_6.176608, whole genome shotgun sequence.
ACCESSION   DAOPNH010000197 DAOPNH010000000
VERSION     DAOPNH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_6459_cov_6.176608"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2527)
                     /locus_tag="PKZ74_10355"
     CDS             complement(<1..2527)
                     /locus_tag="PKZ74_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPM93201.1"
                     /translation="MKSLRLITLFLILGLHFSGISQVSREWTVVASYPIPGKASGLAW
                     DGTYLYSGLYSSPGDDNLVYRIDPSDGTYNLQCAGPFVQAYGLTFDGTYLWTTDHPSS
                     SQPANAICFDMSGNQVTTFALPATYMSGIAWDNNRFWACAYYNPDGMTYKLNSTGGII
                     TQFPSPNLQPWDICRQNEFLWVADYNANMLYKTDTLGNTLESHPSVGLKPSGVVYDGQ
                     FLWYCDGELQAQSTLYKIDLGGSGTPEIFLPENEHDYGIVTIGESSTWNMNVENTGDG
                     DLVITGLLIPDAAPVFTTFTPPQTITPGHSISIPLTYQPAEPIPLNTEITVVSTDPVD
                     PEVPVYLTGNAVLNGPHLLASETTHDYGTVRMEAWTRWFLYISNNGSGTLTVGSINIT
                     SENFILDGGTVFPLNISSLDTARVGVWFNPENDIQYAGSMEITHNGTNENPYIIDLAG
                     AGIDQDYPIGDLLWYYVIDVDYDNSPKAIVPIEDVTGDKESDVIVSSEDDILRCFNGN
                     SHNLADVIWERPIYAGCAYGQNSLTVIDDIDGDTYQEVIIGTAWGDRSVTAFSGKTGE
                     QIWKYDTHEYGDGGWVYQVDARFDYNNDGVKDVLAATGDDANDTGPRRIFCLDATDGG
                     KIWECFTGGPNFAIIGVEDFTGDNIPDAVAGASNNSESQGKVYGINGSNGAISWTYTT
                     EGSSVWAVEQLLDINNDGVRDVAAGDFSGHILYLDGTDGSVIATGYIGNHLILRFEWI
                     EDVNSDSHPDILVANSGINTVMLNGLTAESLWQHPMPDKPWNLAAANDLNGDGISDVM
                     VGTLYSNNYLFFLDGITGDELESISFGEAVDAINAIPDIVGDGS"
     gene            2705..4714
                     /locus_tag="PKZ74_10360"
     CDS             2705..4714
                     /locus_tag="PKZ74_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPM93202.1"
                     /translation="MTLKLIETDPWLEPSRQDIEDRYNRFRNRLEGIAKDYISLERFA
                     DGHRYFGINFDEERDGWIYREWAPQASGLYLTGDFNKWERFSHPLAKNDFGTWEIFLD
                     RNTYQDSFLHGTKIKVLVDSAKGLRYRIPAYIRRAVQDDDTKNFSGQLWFPEPFDWSG
                     DRFDGSRLGELCIYEGHVGMAQEKEGIGTYDEFTESILPRIKNAGYNSIQLMAIQEHP
                     YYGSFGYHVSNFFAPSSRFGTPEELKKLIKTAHQMGIAVILDVVHSHTVKNVVEGLNE
                     FDGSDAQYFHPGGRGEHPQWDSKIFDYGKTEVLRFLLSNLKYWMEEFHFDGFRFDGVG
                     SMMYYHHGLEIIDSRDKYFRQGVEWDALTYLQLANALIHKLNPAAVSIAEDVTGMPGL
                     TFPVDEGGLGFDYRLGMGIPDFWIKYLKEYRDEDWSIHEMWHVLNDRLPHVKTVTYAE
                     SHDQALVGDKTIAFHLMDKEMYWHMHKDDPDLVIDRGMALHKMIRLFTIALGGQAYMN
                     FMGNEFGHPEWIDFPREGNGWSYKYARRQWSLVDSPDLKYQYLAAFDKEMIRIMKEHQ
                     VMTSLFGNQLNMDEANKTIVFERQGLIFVFNFHPTHSIADYAFPVPRHGEYRIILNSD
                     SKAFGGHGRIDDSITYYTFSDPSGNWPYLKIYNTNRTALVLKFDL"
     gene            complement(4740..6032)
                     /locus_tag="PKZ74_10365"
     CDS             complement(4740..6032)
                     /locus_tag="PKZ74_10365"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM93203.1"
                     /translation="MKNFLLTLALVLFSLSVYSQWYQHTDIPVSTNLSGITFIDSFHG
                     WILGDSGTILHTNDGGSTWEIQYCPVGVSFGDISMIDSLNGWIVGHGPDTSGYILKTT
                     DGGTNWILQDVSLHNLQDVCFTDSLHGWMIGESGERLPCMLRSTFDGGATWSEAIINE
                     VQIPYDVIFTDSMTGFIGGTYHNGSDLCARIIKTTDGGATWQVKLNWCSDPFCFYNDM
                     AFANDSVGWLVGESWIEGIHGAEADFGFIMATQDGGETWHEIFSDEIFPVEACLLSDQ
                     SGWAVGGDGFFMYTGNGGNTWDMMNLPPEESLYDIFFIDDDTGWAVGTYGTVMYTNNN
                     GTMEVPEVPDDPTVLKAVPNPAHGELTMNFTVDKGGPATVELVDLSGRTVVQMPGKML
                     FDGPNRLTLQWGNEVTPGIYFLRIVSTSGILTGKILVE"
     gene            6159..6401
                     /locus_tag="PKZ74_10370"
     CDS             6159..6401
                     /locus_tag="PKZ74_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPM93204.1"
                     /translation="MKPITFTSTLPAEIWQSLEAYAEKFKVPRNRIMEQALKAYFERL
                     KQAEYIHSFRKAAGDPEMGAMAAEGLADYLKILDEP"
BASE COUNT         1684 a         1625 c         1548 g         1602 t
ORIGIN      
        1 acgagccgtc gccgacaatg tcgggtatgg cgttgatggc gtccaccgct tcaccgaaag
       61 atattgattc cagctcatca cccgtgatcc catccaggaa aaacaggtaa ttgttgctgt
      121 aaagcgtccc caccattaca tcgctaatgc cgtcaccgtt cagatcattg gctgcggcaa
      181 ggttccaggg tttatcaggc atcgggtgct gccataaact ttcagcggta aggccattca
      241 gcataacggt attgataccg ctgttggcta ccaggatatc cgggtgcgaa tcgctgttga
      301 catcctcgat ccattcaaat ctcaggatca ggtggttacc gatatatccg gttgcgatga
      361 ccgatccatc cgtaccatcc aggtaaagga tatggccact gaaatctcca gccgccacat
      421 ccctgacccc atcattgttg atatcgagca attgttccac agcccatacc gaagatcctt
      481 ccgttgtata ggtccagctg atggctccgt tgctcccgtt tataccgtat acttttccct
      541 ggctctccga attgttggag gcgccggcta ctgcatccgg gatattatct ccggtaaaat
      601 cctcaacacc gatgatcgca aaattcggac ccccggtaaa acactcccat atctttcccc
      661 cgtctgttgc atccaggcag aatatccggc gcggcccggt atcattggca tcatcacctg
      721 tagctgcgag tacgtcttta actccgtcat tattataatc aaaccgggca tcgacctggt
      781 aaacccagcc cccgtcgcca tattcgtgcg tgtcgtattt ccagatctgt tcaccggttt
      841 tacctgaaaa ggctgtcacc gaccggtcac cccaggctgt gccgataata acctcctggt
      901 aggtgtcgcc atcgatatcg tcaataaccg tcagtgaatt ctggccatat gcgcaaccag
      961 catatatcgg gcgttcccag atcacatctg ccagattgtg ggaatttcca ttgaaacaac
     1021 gcaggatatc atcctccgaa cttacaatca catcgctttc tttatccccc gttacatcct
     1081 caatagggac aatggctttc gggctgttgt cgtaatccac atcaatgaca taataccaga
     1141 gaagatcacc gatgggataa tcctggtcga tgcctgcacc cgccaggtcg ataatatatg
     1201 gattttcatt cgttccatta tgagtaatct ccatcgagcc tgcatactgg atatcatttt
     1261 cgggattgaa ccatacgcca acccgggcag tgtccaggct ggatatattc aacggaaata
     1321 ccgttccgcc gtcgagaata aaattttcag aagtgatgtt tattgaaccg actgtcaatg
     1381 ttccgctgcc attattactg atgtaaagga accagcgggt ccaggcttcc atcctgactg
     1441 taccgtaatc atgagtagtt tccgaagcca gcaggtgtgg gccatttaaa accgcattgc
     1501 cggtgagata caccggcacc tccggatcaa caggatctgt tgaaacgact gtgatttcag
     1561 tgttcagggg gattggttcc gcaggctggt aggttagggg aatggaaatg gagtgcccgg
     1621 gggtgatcgt ctggggcggt gtgaatgtcg tgaaaactgg cgctgcatcc gggataagaa
     1681 gaccggtgat gaccaaatcg ccatctccgg tgttctcgac attcatgttc caggttgatg
     1741 attcaccgat cgttacaatt ccataatcgt gttcattttc aggcaaaaat atttcagggg
     1801 ttcctgatcc tcccaggtca atcttatata aagtactttg agcctgcagt tcaccgtcgc
     1861 aataccatag gaattgcccg tcataaacca cgccggaggg ctttaatccc acggaaggat
     1921 gactctccag ggtgtttccc agggtatcgg tcttataaag catgttagca ttgtaatcgg
     1981 caacccacag aaactcattc tgcctgcaga tatcccatgg ctgcaggttg ggtgaaggaa
     2041 attgagtaat gatcccgccg gtcgaattca gcttataggt cattccgtca ggattataat
     2101 aggcacaggc ccagaaacgg ttgttatccc aggcgatgcc cgacatgtac gttgccggta
     2161 gggcaaatgt agtgacctgg ttgcccgaca tgtcgaaaca gatcgcattg gcgggctgcg
     2221 aagaactcgg atggtcggtg gtccaaaggt aagttccgtc aaatgtcaat ccataggcct
     2281 gtacaaatgg ccctgcacac tgaagattgt atgtcccgtc cgatggatca atgcggtaga
     2341 ccaggttatc gtcaccggga gaggaataaa gtccgctgta aagataggtg ccatcccatg
     2401 ccaggccgct ggctttaccc ggaatagggt aggaggcgac gactgtccat tcgcgggaaa
     2461 cctgcgaaat acctgagaaa tgaagaccca ggattaaaaa tagtgtaatg agtcttaagc
     2521 ttttcatgtg agtctgattt ggatttcagg gttaaagata atagtatctg cttaaactat
     2581 aaactttgaa ctttgaactt tgaactttaa actttgaact ttgaactttg aactttgaac
     2641 tttgaacttt gaactttgaa ctttgaacta atctttctat ctttatccaa ttcaaaaccc
     2701 ttgtatgact cttaaactca tcgaaaccga tccctggctt gagcccagca ggcaggatat
     2761 cgaagacagg tataaccgct tcaggaacag gcttgaaggt attgctaaag attatataag
     2821 cctggaaaga tttgcggatg gacaccgtta tttcgggatt aattttgatg aggaaagaga
     2881 tggttggatt taccgggaat gggcaccaca ggcatccgga ctatacctga ccggtgattt
     2941 taacaaatgg gagcgctttt ctcatccgct cgcaaaaaat gattttggca cctgggagat
     3001 tttcctcgac aggaatactt accaggattc cttcctccat ggcactaaga tcaaggtgct
     3061 ggtcgattcg gcaaaaggat tgcgctaccg cattccggcc tatatccgcc gggccgtgca
     3121 ggatgacgac actaaaaatt tctcaggcca gctctggttt ccggagccgt tcgactggtc
     3181 cggcgaccgg tttgatggca gccggctggg tgagctctgt atctatgaag gccacgtggg
     3241 catggcacag gagaaggaag gcatcggaac gtatgatgaa ttcacggaat ccatcctgcc
     3301 aaggattaaa aacgccggct ataattccat ccagctcatg gccatccagg agcatcctta
     3361 ttatggctcc ttcgggtacc atgtatccaa tttcttcgct ccttcatccc gtttcgggac
     3421 gccggaagag ctgaaaaagc ttatcaaaac agcccatcaa atgggtattg ccgtgatcct
     3481 cgacgtggtc cattctcata cggtgaaaaa tgttgtcgaa ggattgaatg aattcgatgg
     3541 ttccgatgca caatattttc acccgggagg gagaggagag cacccgcagt gggactccaa
     3601 gatttttgat tatggtaaaa cggaagtact gcgctttctg ctttctaacc tcaaatactg
     3661 gatggaagag ttccatttcg acggcttccg gttcgatggg gtcggctcga tgatgtacta
     3721 tcatcacggg cttgaaatta tcgattcgcg tgataaatac ttccgccagg gagtggaatg
     3781 ggatgcgctg acttatctgc aactggccaa tgccctgata cataagctta acccggctgc
     3841 agtctccatt gcagaagatg tgaccggaat gcccggcctc accttccctg ttgacgaagg
     3901 cggacttggt tttgactacc gcctcggaat gggtatcccc gatttctgga tcaaatacct
     3961 gaaagaatac cgcgacgaag actggagcat ccacgaaatg tggcacgtgc tcaacgaccg
     4021 cctgccgcac gtgaaaaccg taacctatgc cgaatcgcac gaccaggcgc tggtgggcga
     4081 taaaaccatc gcattccacc tgatggataa ggagatgtac tggcatatgc ataaggacga
     4141 ccctgacctg gtcatcgacc ggggtatggc gctgcacaaa atgatccggc tcttcaccat
     4201 cgccctggga ggtcaggcct acatgaactt catgggcaat gagttcggcc atccggagtg
     4261 gatcgacttc ccgagagaag gcaacggatg gagttataaa tatgcccgca ggcagtggtc
     4321 gctcgtcgac agccccgacc tgaaatacca gtacctggcg gcattcgaca aggaaatgat
     4381 caggatcatg aaagagcacc aggtgatgac atcgctgttc ggcaaccagc ttaatatgga
     4441 cgaagccaac aaaacgatcg ttttcgaaag gcaggggctg atcttcgttt ttaatttcca
     4501 cccgacgcat tccattgctg attatgcttt cccggtgccc cgccacggag aataccggat
     4561 catactgaat tccgacagca aagcgttcgg cggccacggc cggatagacg attcgattac
     4621 gtattatacc ttttccgacc catcaggcaa ctggccttac ctgaagatct ataacacgaa
     4681 ccgtacggcc ctcgtgctga agtttgatct gtgaagcctt taaaatagat cagaagaccc
     4741 tattcaacaa ggatctttcc ggtcagaatg cctgaagtgg agacgattct caggaaataa
     4801 atacccggcg taacctcatt tccccactgg agggtcaacc ggttcggacc gtcgaacagc
     4861 attttgccgg gcatttgcac aacggtcctt cccgacaggt caaccagttc aacggttgca
     4921 gggcctcctt tatccacggt gaagttcatc gttaattccc cgtgcgcggg atttggaacg
     4981 gctttcaaca ctgtcggatc atccggcacc tccggcacct ccattgtccc gttattgttt
     5041 gtgtacataa ccgttccata ggtcccgacc gcccagccgg tatcatcatc gataaagaaa
     5101 atatcataca gactctcttc cggaggtagg ttcatcatgt cccaggtatt tccgccattg
     5161 ccggtataca taaaaaatcc gtcaccgcca accgcccatc cggattgatc cgacagcaga
     5221 caggcctcaa ccgggaagat ttcatcggag aagatttcat gccaggtttc tccgccgtcc
     5281 tgggtagcca ttataaagcc gaagtctgct tcagccccgt gaatgccttc aatccagctc
     5341 tcgccaacaa gccagccaac cgaatcgttt gcaaatgcca tgtcgttgta aaagcagaaa
     5401 ggatctgagc accagtttaa tttcacctgc caggttgccc ctccatcggt ggttttaatg
     5461 atcctggcgc ataaatcgct tccattgtgg taagtgccgc cgataaaccc tgtcatcgaa
     5521 tccgtgaaaa taacgtcata tgggatctga acctcattaa taatagcctc tgaccaggtc
     5581 gccccgccgt caaaggtcga tctcaacatg cagggcagcc tttcgccgga ttcaccgatc
     5641 atccagccat gcagcgaatc cgtaaagcaa acatcctgaa ggttgtgtag cgaaacatcc
     5701 tgaaggatcc aatttgtacc accgtcagtc gtttttaaga tgtacccgct cgtgtcgggg
     5761 ccatgaccga caatccagcc attgaggctg tcgatcatgg aaatgtcacc aaatgaaact
     5821 ccaaccgggc aatattgaat ctcccaggtg ctcccgccgt cattcgtatg aagaattgtt
     5881 cctgaatccc cgaggatcca cccgtgaaag gaatcgataa aggtaatccc ggagagattt
     5941 gtactaacag ggatatcagt gtgctggtac cactgtgaat agactgataa cgagaacaga
     6001 accagtgcca gggttaacag gaagtttttc atggcgaaag gaattattcc caaatataag
     6061 gataaaaggg ataaaaacat gttccgtttt acttttttca tttttcattt atgcgtataa
     6121 attataaata ttgattatat ttgtaacaaa atatttatat gaaaccaatt actttcacca
     6181 gcacccttcc ggcagagatc tggcaatcgc tggaagccta tgcggagaag tttaaggtgc
     6241 cccggaacag gattatggaa caagccctga aggcttattt tgaaagactc aaacaggcgg
     6301 aatatatcca ttcttttaga aaagctgccg gagatccgga aatgggtgca atggcagcag
     6361 aaggactggc agattatctc aagatccttg atgaaccatg aaacagcggg aaatatggat
     6421 ggccgatctt aacccggtca agggaagcga acagcaggg
//
LOCUS       DAOPNH010000198         6443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_13800_length_6443_cov_6.691766, whole genome shotgun sequence.
ACCESSION   DAOPNH010000198 DAOPNH010000000
VERSION     DAOPNH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13800_length_6443_cov_6.691766"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(498..1550)
                     /locus_tag="PKZ74_10375"
     CDS             complement(498..1550)
                     /locus_tag="PKZ74_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM93205.1"
                     /translation="MLELRNISKSYPDFKLHDISFRVEDGDYFIILGPSGAGKTQILE
                     IIAGLVHPDEGGMFIDERDITEERIQSRGIGIVFQDYAVFPHMKVRENLAYSLRRTGY
                     EKGQIARLVAEQAEAMEISHLLDRRPSTLSGGELQRVALARTLIRKPRYLLLDEPLAS
                     LDVSLRQGLRSLLKQLNQAGQTIVQVTHDYEEALVLADKVAVIHQGKIIQSGKADDVF
                     HHPGSEFVARFTGIRNYFPARLFREEDRQYGELENGAILRLATDETEGRGFILIRGED
                     IFISLEDLPTSATNRFPGRIAGISPSVYGFEVLVDTGTLYYVRITRESLQNLGLHEGK
                     EVWISFKASNVRFVRV"
     gene            complement(1543..2340)
                     /locus_tag="PKZ74_10380"
     CDS             complement(1543..2340)
                     /locus_tag="PKZ74_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93206.1"
                     /translation="MYFRFIMRFQPFQLFLILLSGVVLLFIIGPLLGMFISTTPYQLF
                     ETSKDPEVQRSIGLTLWVSMAATLVFALGGIPLAYLLARKDFALKKVILGIIDLPIVI
                     PHSAAGIAVLGFISRETVVGKMGSAIGLDFVGAPAGIAIAMGFVSIPFLVNAARDGFL
                     AVPVRYEQAALTLGASPIRVFFTISLPLAWRNIVSGLILMFARGMSEFGAVIIIAYHP
                     MVAPVLIWERFSSFGLSYARPVAVLFILVSLVFFIGLRFLSRTKKDA"
     gene            2481..3197
                     /locus_tag="PKZ74_10385"
     CDS             2481..3197
                     /locus_tag="PKZ74_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM93207.1"
                     /translation="MGWLYDLMSKDVRFVEGLKACINCGTCTAICPAAEFYNYDPRIL
                     VTTLQTRDDEKIEALLKSDFIWYCGECMSCKTRCPRGNVPGGLISVCRSFSQDLGYFI
                     ESEKGRQQLAVKRTIGEWILDHGYCLFPQQIEPESHPEQGPVWEWITNSLKEVYGRFD
                     ATYQEEGPGILRKIPQDSLDELKRIFDVTGGTERFEKLEEYSKKKAEEMGLEFSPGQK
                     SEYYLHVYNFNSNKHGKDEH"
     gene            3187..4260
                     /locus_tag="PKZ74_10390"
     CDS             3187..4260
                     /locus_tag="PKZ74_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPM93208.1"
                     /translation="MNIEGKKIIWSQYQKEIADNDYFYARSCVRQSFFPGSETTFMKI
                     VRDDLGKNISEHPFHTTCTGIAYHSDVVPFETTMTVIARQFALMTESGFQNYVPSCIT
                     SFGMYNEVLETWHHFPEVEMKVRKMLKEATGKEFEKPKNLVHSSDIIYKFRKEIAEKG
                     KYRLINRFTGEPIRVVEHIGCHYSKMFPSKGVGGAEYPHVLVGMIEEWGGEVVDYPER
                     RHCCGFGFRQYVVKANRGYSIACSKKKFESMQPYRPEAILTNCPGCPFFFDRWQYAIS
                     EMEGKTYGENGQGIPVLTYEELAGIILGYDPWDLGFQTHQVPVEPLLDKIGIPYDPEK
                     KFLGKNGEFIGRPDMPDNLKFCH"
     gene            4264..5286
                     /locus_tag="PKZ74_10395"
     CDS             4264..5286
                     /locus_tag="PKZ74_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM93209.1"
                     /translation="MTTKVVAVIGGGAAGLEAAANLAGFGYTVHLIEKEHQPGGHVKE
                     WDRLFPSRRRASEIIGHLGGQLNENVRIHLGKEIINIRRDNGSWRIGLNDESRLDAQA
                     VILATGFDLFEAAKKEEYGYGIYDNVITSADLERMFREKGEIRNHQGMIPQRVGFVHC
                     VGSRDEKVGNLYCSRVCCVTAVKQAIEVCEMIPGCEVFCFYMDLRMFGLDFEALYKEA
                     QEKWGIHFIRGRLSEAFENPDGSVLVKVEDTLAARPLKMKTDLLVLMSGMVPNAGTKR
                     LVEMLGLQTDENRFLLPADRQLRANETGIPGIFVAGTCTSPKSIDETLADARAAAIKV
                     MEYFRE"
     gene            5343..5678
                     /locus_tag="PKZ74_10400"
     CDS             5343..5678
                     /locus_tag="PKZ74_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM93210.1"
                     /translation="MKNFGYAINSDRQIDYDSNDHRVARYLAEHEPSFRVCFACGTCS
                     ATCSAGLFTDLSLREMIGNIKRGEISKIKSEAEKCMLCGKCQLACPRGVNTRNVILTI
                     QRALVKYNL"
     gene            5675..>6443
                     /locus_tag="PKZ74_10405"
     CDS             5675..>6443
                     /locus_tag="PKZ74_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM93211.1"
                     /translation="MTFDFFVLPFFLGLITLLVILAVKYSRWIREFDPADKALIKKNI
                     FTTKSLRAAGEVFMESLLHRKMFRKNAMLGYMHMSFAMGWFLLIVCGNLESRIYSKVH
                     LNPPYYPIFLKFFVHDHRVLAHELLSIPGVFRVLMDAILLMILSGLALALFKRASSRW
                     FGMRKASKHSLYDRIAITTLWLIFPLRLLAESFTAATYEGGSFLTNSFGSFLGSFLPA
                     ETLAYPAWWAYSLALGGFFVSLPYSRFMHIPTEIMLTK"
BASE COUNT         1684 a         1591 c         1715 g         1453 t
ORIGIN      
        1 gcaagaaaat agcatggcta agaatagaaa taagatccaa ttggttaaag aagaattctc
       61 gaaatggatg aaacaaatat aattgtattc aatacaaaag tcaagggtgg ttgataactt
      121 tttctgttcc aggagaaaat gaagtgagac tccatctcat atcgggcaga tggagtcccg
      181 gttgagatga gactccatct caaccagagc agatggagtc tcgttaaaat gagatggagt
      241 ctcaaagaat tacccgattc ggtattgtaa cagggtttcc aacctttttg ctccacacaa
      301 tcgaatgaat tatagaaagt tatagagatg cttaaatatt gttataaatt atcggatatt
      361 taaaaatcac ataatcagta tgttacaatt tatataacga tttccaatta atagggttgg
      421 aaaccctgtt tctacaactg tccaactgcg atccttcgtc cgccaaaggc ggattcagga
      481 catgctgtcc aactgaccta tacccgcaca aacctcacgt tgctggcttt aaagctgatc
      541 cacacctctt tgccttcgtg caggccgaga ttctgcaacg attcgcgggt gattcgcacg
      601 tagtaaagcg ttccggtatc gacaagcacc tcgaagccgt agaccgacgg actgatcccc
      661 gcaatacggc ccggaaaccg gttggtggcg ctggtcggca gatcttccag cgaaataaaa
      721 atatcttctc cccggataag gataaacccg cgcccttccg tctcatcggt cgccagccgc
      781 aaaatggcgc cgttctccag ctcgccgtac tgccggtctt cttccctgaa cagccgtgcc
      841 gggaaatagt tgcggatgcc ggtgaaacgg gcgacaaact ccgaaccggg atggtgaaac
      901 acatcatcgg cctttcccga ctgtatgatc ttaccctggt ggatcacggc aactttatcg
      961 gccaggacaa gcgcctcttc ataatcatgg gttacctgca cgatcgtctg cccggcctgg
     1021 ttcagttgct tcagcaggct tctcaaaccc tggcgcagcg aaacatcgag cgatgccagc
     1081 ggctcatcaa gaagcaaata acgcggcttc cggatcaggg tgcgggccag cgccacgcgt
     1141 tgcagttcgc cacccgagag ggtcgacggc cggcggtcga gcaaatgact gatctccatg
     1201 gcctccgcct gctccgccac caaccgggcg atctgacctt tttcgtaacc ggtgcggcgc
     1261 aacgaatagg ccaggttctc cctgactttc atgtggggga aaacggcata gtcctggaat
     1321 acaatcccta tcccgcgcga ctggatcctt tcttccgtaa tatcccgctc atcgataaac
     1381 atccctcctt cgtcgggatg aacaaggccg gcgatgatct cgaggatctg cgtcttgccg
     1441 gcacccgacg ggcccaggat gatgaaataa tcaccatctt ccacccggaa ggagatatca
     1501 tgcagcttga aatcaggata tgattttgat atgttcctta gctcaagcat ctttttttgt
     1561 cctcgataaa aatcttaatc cgataaaaaa cacaaggctg acaaggataa acaagactgc
     1621 taccggccgg gcataactca gcccgaacga gctgaacctt tcccagatca ataccggcgc
     1681 taccatcggg tggtaagcaa taataatcac ggcgccgaat tcgctcattc cgcgggcgaa
     1741 catcaggatc agcccggaga cgatattgcg ccaggccagc ggtagcgaga tggtgaagaa
     1801 cacccggata ggagatgccc ccagggtcag cgccgcctgc tcgtaacgta ccggcacggc
     1861 caggaatccg tcgcgtgcgg cgttgaccag gaacggtatg ctgacaaatc ccatcgctat
     1921 ggcaatgcct gccggcgcac cgacaaagtc gaggccgata gctgaaccca ttttcccgac
     1981 gaccgtttcg cgcgagatga agcccagcac cgcaataccc gcggcggaat gggggataac
     2041 gatcggcagg tcgatgatcc ccaggatgac ttttttgagg gcaaaatcct ttcttgccag
     2101 aaggtaagcc agcggaatcc cgcccagggc gaataccagc gtcgccgcca ttgataccca
     2161 taatgtcagc ccgatgctcc tttgcacctc gggatctttc gatgtctcaa aaagctgata
     2221 gggtgtcgtc gaaataaaca tccccagcag cggcccgatg atgaataaaa gaaccacgcc
     2281 gctgagcagg atcagaaata gctgaaaagg ctgaaaccgc atgatgaaac gaaagtacaa
     2341 atttttccga accgaaggga cgctcgccgg actaccttat attttggcaa aaaatgtatg
     2401 gctaaatgaa tatgcattaa tttcatcatt caattttact acatttgtta aagaattaac
     2461 aatagtttaa gtctgtcttt atggggtggt tatacgatct gatgagcaaa gatgtcaggt
     2521 ttgtcgaagg cctgaaagcc tgcatcaatt gcggaacctg cacagccatc tgcccggcgg
     2581 cggaatttta taactacgat ccgcgcatcc tggtcaccac ccttcaaacc cgcgacgacg
     2641 aaaagataga agccctgctg aaaagcgatt tcatctggta ttgcggcgaa tgcatgtcgt
     2701 gcaaaacccg ctgtccacgc gggaatgtcc cgggaggact gatctcggtt tgccggtcgt
     2761 tttcgcaaga cctgggctac tttattgagt ccgagaaagg aaggcagcaa ctggccgtaa
     2821 aacgtaccat cggcgagtgg atactggatc atggctattg cctgttcccg cagcagatcg
     2881 aacccgaatc tcatcccgaa caggggccgg tatgggaatg gatcaccaac agcctgaaag
     2941 aggtctatgg ccggttcgat gccacttacc aggaggaagg acccgggatt ttacgcaaga
     3001 tcccgcagga ttcgctggat gaactgaaac ggatcttcga tgtgaccggt gggactgaac
     3061 gatttgaaaa gctggaggaa tattcgaaga agaaagccga agagatggga cttgagttca
     3121 gcccgggaca gaaaagcgaa tactatcttc acgtatataa ttttaacagt aataagcacg
     3181 gcaaggatga acattgaggg gaaaaaaata atctggagcc agtaccagaa ggagattgcc
     3241 gataacgatt atttctatgc ccgaagctgc gtgaggcaga gctttttccc gggttcggaa
     3301 acgactttta tgaagattgt aagggatgac ctgggtaaaa atatctcaga gcatccattc
     3361 catacgactt gcacagggat cgcctaccat tctgatgtcg ttccatttga aactacaatg
     3421 acggtcatcg cccgccagtt cgctcttatg accgaatcgg gctttcagaa ttatgtccct
     3481 tcctgtataa cgtcattcgg aatgtataac gaggttctgg aaacatggca ccatttcccc
     3541 gaggtggaga tgaaagtgcg gaagatgctg aaagaggcca cggggaaaga gtttgagaaa
     3601 ccgaagaacc tggtccattc cagcgatatc atatacaaat tccggaaaga gatcgcagaa
     3661 aaaggaaaat accggctcat taaccgcttt accggtgaac cgatccgcgt ggtggagcac
     3721 atcggatgcc actattcgaa gatgttcccg agcaaagggg ttggcggggc ggaatatccg
     3781 catgtccttg tcgggatgat cgaagagtgg ggaggagaag tggtcgacta cccggaacgc
     3841 cgccattgtt gcggctttgg atttcgccag tacgtggtca aagccaaccg tggctattcc
     3901 attgcctgct ctaagaagaa atttgaatcg atgcaaccct accggcccga agcgatcctg
     3961 accaactgcc cgggctgccc gttcttcttc gaccgctggc agtatgccat cagcgaaatg
     4021 gaagggaaaa cctatggcga aaacgggcag gggatacctg tccttaccta cgaagaactg
     4081 gccgggatca tcctgggtta tgatccctgg gacctgggct ttcagacgca ccaggtgccc
     4141 gtggaaccgt tactggataa aattggaatt ccttatgatc ccgaaaagaa atttcttgga
     4201 aaaaacggtg aatttattgg tagaccggac atgccggata atctcaaatt ttgccattga
     4261 cctatgacca cgaaagtagt tgctgtaatc gggggtggcg ccgcaggact ggaggcggcg
     4321 gcaaatcttg cagggttcgg ctataccgtt catttaatag aaaaagaaca tcagccaggc
     4381 ggtcacgtca aagaatggga ccggctgttt ccttctcggc gccgggcatc tgagatcatc
     4441 gggcatctcg gagggcaact caacgaaaat gtcaggatcc acctggggaa agagatcata
     4501 aatataagaa gggataacgg atcttggagg atcggactga acgatgaatc gcgattggat
     4561 gctcaagctg tgatcctggc cactgggttc gacctgttcg aagccgcgaa aaaggaagaa
     4621 tacggttatg ggatttacga caatgtcatc acttcggccg acctggagag aatgttcagg
     4681 gagaaagggg agatcaggaa tcatcagggg atgattccgc aacgggtggg atttgtccat
     4741 tgcgtgggat ccagggatga aaaggtgggc aatctttatt gctcccgggt ttgctgtgtg
     4801 acggccgtca aacaggccat cgaagtctgt gaaatgatcc ccggatgcga agtgttttgt
     4861 ttttatatgg acctgaggat gttcggactc gatttcgaag cgctttacaa agaagcccag
     4921 gagaaatggg gcatccactt tatccggggg aggctttcag aggctttcga gaatcccgac
     4981 ggatcggtgc tcgtaaaggt agaagacacc ctggccgcgc gcccgttgaa aatgaaaacg
     5041 gacctgctgg tgctgatgtc gggcatggtg cccaatgccg gcacaaaacg gctggtggag
     5101 atgctgggcc tgcagacgga tgaaaaccgt ttcctgctgc ccgccgaccg ccagctccgg
     5161 gccaacgaga ccggtatccc cggtattttc gtcgccggca cctgcacctc tccgaagagt
     5221 atcgacgaaa cgctcgccga tgcccgggcg gcagcgatca aagtgatgga gtatttcagg
     5281 gaatagtgaa tagcgctctg atacatggac aatggacaat ggacaatgga caatagtgaa
     5341 tagtgaaaaa tttcggttat gcgataaatt ctgaccggca gatcgattac gacagtaacg
     5401 atcaccgggt tgccaggtac ctggcggagc atgagccgtc gttccgggtc tgctttgcct
     5461 gcggcacctg ctcggcgacc tgcagcgccg gattgtttac cgatctcagc ctccgggaga
     5521 tgatcgggaa tattaaaaga ggtgagatta gtaaaattaa atccgaagcg gagaagtgca
     5581 tgttgtgcgg aaaatgccag cttgcctgcc cgcggggagt caataccaga aatgtgatcc
     5641 tgaccattca gagagctttg gtgaaatata atttatgacg ttcgacttct ttgtcctgcc
     5701 gtttttcctg ggcctgatca ccctgctggt gatactggcg gtgaaatatt cccgctggat
     5761 cagggaattt gacccggcag acaaagcgct cattaagaaa aacattttca cgacaaaaag
     5821 cctccgggcg gcaggagagg tattcatgga gagcctgctg caccggaaga tgttccggaa
     5881 gaatgccatg ctgggctata tgcacatgag ctttgccatg ggctggttcc tgctgatcgt
     5941 ttgcgggaac ctggaatcga gaatatacag caaagtccac ctgaatcccc cttattatcc
     6001 gatatttctg aaatttttcg tgcacgacca ccgggtgctg gcccatgagc tgttatccat
     6061 accgggcgtt ttcagagttt tgatggatgc cattttgctt atgatcctgt cggggctggc
     6121 gctggcgctg ttcaaaaggg cgtcatcacg ctggttcgga atgcgaaaag ccagtaaaca
     6181 cagcctgtat gaccggatcg ccataaccac gctctggctg atctttccgc ttcgcctgct
     6241 ggcagagagc ttcacagctg caacttatga aggagggagc ttcctgacca acagtttcgg
     6301 cagtttcctg ggaagttttc tgccggccga aacactggcc tacccggcct ggtgggccta
     6361 ttcactggct ctgggcggat tctttgtcag cctgccttat tcccggttta tgcacatccc
     6421 tacggagatc atgctgacaa aga
//
LOCUS       DAOPNH010000199         6357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14055_length_6357_cov_5.117423, whole genome shotgun sequence.
ACCESSION   DAOPNH010000199 DAOPNH010000000
VERSION     DAOPNH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14055_length_6357_cov_5.117423"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKZ74_10410"
     CDS             complement(<1..808)
                     /locus_tag="PKZ74_10410"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPM93212.1"
                     /translation="MERKTALDIHVTGLVQGVGFRPFVYRLAVSNQIYGWVENNNDGV
                     RIHAEGFAGDLEAFRAQLVNEAPRASQIRGIDSDFSTFEGLSGFEIRKSSDLSDEITE
                     ISPDIAVCQDCLEDMKRQENRYNYPFINCTNCGPRFSIIKGVPYDRPKTTMEPFRMCP
                     ECCSEYTDVLDRRFHAQPVACWHCGPRYTYHTAGSVVENPGEILKVLATDLGNGKIIA
                     IKGIGGFHLACDATDETTVRRLRQRKIREGKPFAVMFRDLHTLRSYMEVSQ"
     gene            968..2773
                     /locus_tag="PKZ74_10415"
     CDS             968..2773
                     /locus_tag="PKZ74_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPM93213.1"
                     /translation="MESKIKAIIAKYSHDRTRLMDILHDVKDEFGCISPEAAGLIAGL
                     LKTSQADVEQTISFYHFYSMKPAGKYAVYLNNSAVACMMGRDEIAKTFEKEAGIPFNS
                     VTADGLIGLWDTSDIGMNDQEPAAIINDVVFTRLTPSKVREIVAVMKAGQPIQELVVK
                     YGDGENSHPLIKSMVHNNIMKKGPVLFSEYELGMAIRKAVNLSPELVIDEVKRSNLRG
                     RGGAGFPAGLKWEFCRKSRGAVTYLLCNADEGEPGTFKERVILTELPKQLFEGMVVAG
                     YALGAKEGVLYLRYEYRYLVAYLEQMLQEMRDGNLLGRNIAGKQGFNYDIRIQLGAGA
                     YVCGEESALIESMEGKRGEPRNRPPFPVQKGYLDMPTVINNVETLSAVVKIMVKGADW
                     FKAMGTKESAGTKLLSVAGDCKHPGVYEIEWGTTIRELLEMVGATSVQAVQVGGPSGV
                     CINPTQFHREIAFEDLATGGSMIIIGKQRNLLKEVVVNFMDFFIEESCSSCVPCRSLT
                     VILKNKLQKILDGKGSRLDVNELYEWTKYMKAANRCGLGQTAANPVLSTIENFRQLYD
                     DLVIPEEEFVSTFNLEAAVADSCAYVGRIPKFEHH"
     gene            2801..3502
                     /locus_tag="PKZ74_10420"
     CDS             2801..3502
                     /locus_tag="PKZ74_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPM93214.1"
                     /translation="MSEKIRFIIDDKVCYAKPGQTIMDAARDNGIFIPSLCYLEGIKP
                     AGSCRICNVKVNGRNMTACTTPVAEGMVVENNLPDLEDMRKAIVEVLFVAGNHFCPSC
                     EKSGNCELQALGYRYKMMVPRFPYEFPVKGVNAKAKYIYHDRNRCILCKRCIRTVMKD
                     DKPVFAFNQRGGHHLHIEMDIELANSLTEAEALHAMDICPVGAIIKKERGYYSPIGSR
                     KYDFDEIGADLVKKV"
     gene            3527..4075
                     /locus_tag="PKZ74_10425"
     CDS             3527..4075
                     /locus_tag="PKZ74_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93215.1"
                     /translation="MSKAIVATTSMAGCFGCHMSILDIDERILDLIELVEFNKSPIDD
                     IKKFTKQCDVGILEGGCCNSENVEVLKEFRKNCKILISVGECAIMGGLPALRNGIPIQ
                     ECIEEAYLNGPTVGLNKDRILPNDDELPMLLDKVYPCHEIVKVDYFLPGCPPRADLIW
                     NALVALLTGDEMKLPYEVVKFD"
     gene            4092..5543
                     /locus_tag="PKZ74_10430"
     CDS             4092..5543
                     /locus_tag="PKZ74_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPM93216.1"
                     /translation="MTKKITIEPVTRVEGHGKVTIHLDEQNNVAQTRLHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKALDVIVGAGTGEGLTPTGEKMRRLMHY
                     GQMFQSHSLHFFHLVSPDLLLGIDADPAIRNVIGVAIHHRDLAVQGVMMRKFGQEIIA
                     ATAGKKIHGTGAIPGGINKHLTIEERDKFLKGADPLNIDKMIEWSIGALDFFKSYYKK
                     NKEFIDSFAYFPSNHLSLVRKDGALDLYHGVLRAVDTDGKKILNDIDYQYYADYIEEE
                     VRSWSYMKFPYLKELGPQKGWYRVGPLARLNTCDFIPTPLAQKEFEEFKSLTNGKPNN
                     HCMHYHYARLIEILHSAEEIKKLLNDPDLLEGPLVAKGQKLNEGVGLIEAPRGTLFHH
                     YRINEFDQITMANLIVSTTNNNEPMNQSVNRVAKSWMNGKTEITEPMMNAVEVAIRAY
                     DPCLSCATHALGQMPLDISLYDKDNNLIDRKRT"
     gene            5546..6025
                     /locus_tag="PKZ74_10435"
     CDS             5546..6025
                     /locus_tag="PKZ74_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPM93217.1"
                     /translation="MAEFFNILIYGYGNPGREDDGLGIELVKQLEQWAAENELQGISF
                     DSNYQLNIEDAEAISDMDVVIFADASTEDIEDFILTKVTGETEVSFTTHAASPGYIVK
                     LCEEIFGKSPEVYLLHIKGYQWDFYEGLSDNAVKNLENALNFISNFILEKLKQNSKP"
     gene            6039..>6357
                     /locus_tag="PKZ74_10440"
     CDS             6039..>6357
                     /locus_tag="PKZ74_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPM93218.1"
                     /translation="MDLSTSYMGLKLRNPLIVSSSRITSTVDDVKKCADNGAGAIVLK
                     SLFEEQFLADSNRLMDQDQKYFWFPEAIDFINQHSREHGIKGYLKLIKGAKANTDVPI
                     IASV"
BASE COUNT         1732 a         1490 c         1583 g         1552 t
ORIGIN      
        1 cctggctgac ctccatgtag cttctcagtg tgtgcaggtc gcgaaacatg acagcgaagg
       61 gtttgccttc cctgatctta cgctggcgta atctcctgac cgtggtttca tctgtggcat
      121 cgcaggccag gtgaaacccg ccaatgcctt tgatagcgat tattttgccg tttcccaggt
      181 ccgtcgccag gactttcaga atttcaccgg gattttcgac gacggaaccg gctgtgtggt
      241 aagtatagcg cggtccgcag tgccagcagg ctaccggttg tgcatgaaac cttcggtcga
      301 ggacgtcagt atattcactg cagcattccg ggcacatacg gaagggttcc atggtggttt
      361 tcgggcggtc atagggaaca cctttgataa tagagaaacg cggcccgcaa ttggtgcaat
      421 taataaaagg gtaattgtac cggttttcct gccgcttcat atcttccagg caatcctgac
      481 aaacggcaat atccgggctg atttccgtta tttcatctga taaatcgctg ctcttcctga
      541 tctcaaatcc ggaaagccct tcgaatgtag agaaatctga atctattcct ctgatttgag
      601 atgcccgggg agcttcgttc acgagttgtg cacggaaagc ctccaggtct cctgcaaagc
      661 cttcagcatg gatcctgacc ccatcgttat tgttctcaac ccatccgtaa atctggttgg
      721 aaacagcaag gcgatataca aatggccgga agccgacccc ctggaccagg cccgtcacat
      781 ggatatccaa tgccgttttt ctctccattt tattaaaagg tgccaaaaat aatacaatta
      841 ctatttgaac ttattctaaa ttcttaaata tagtcgtaag ataaacattt tcaattattt
      901 ttgctttgtt aaagatttca caatacctca ttgtatatca gaatattaat taaaaccaac
      961 ctatacgatg gaatcaaaaa taaaggctat tatcgcaaaa tacagtcatg accgaacccg
     1021 gctcatggat attttgcatg atgtgaagga tgaatttggt tgtatatctc cggaggccgc
     1081 ggggctgatt gccggcttgc tgaaaacatc gcaggccgat gtcgaacaaa ccatttcgtt
     1141 ctaccatttc tactccatga aacctgcggg gaaatatgct gtttacctca ataacagcgc
     1201 tgtggcctgc atgatgggta gagatgagat tgcaaagact tttgaaaagg aggccggtat
     1261 tccttttaat tcggtgacag ccgatggcct gatcggactg tgggatacgt ccgatattgg
     1321 aatgaacgac caggagccgg ctgctattat taatgatgtc gtattcacaa gactgactcc
     1381 ctccaaagta agggagatcg tcgccgtcat gaaagccggt caaccgatcc aggaattagt
     1441 ggtgaagtat ggcgatgggg agaacagcca tcctttgata aaatcgatgg tgcataacaa
     1501 catcatgaaa aaagggccgg ttctcttttc cgaatatgaa cttggaatgg ccatccggaa
     1561 ggctgtgaat ctttcccctg aactggtcat tgacgaagtc aagcgttcaa acctcagggg
     1621 ccgcggcggt gccggattcc ccgccggtct gaaatgggag ttctgccgca agtcaagagg
     1681 ggcagttacc tatttactct gtaatgcaga cgaaggtgaa cccggtacct tcaaagaaag
     1741 ggtcatcctt accgaactgc ccaaacagct tttcgaaggt atggttgtcg ccggatatgc
     1801 cctgggcgct aaagaagggg tactttacct gcggtatgaa taccgttacc ttgttgctta
     1861 cctcgaacaa atgcttcagg aaatgcggga tggaaacctg ctgggcagaa atatcgccgg
     1921 caagcaggga tttaattacg atatccggat ccagttagga gccggcgcat atgtctgcgg
     1981 cgaagaatcc gctctgatcg agtcgatgga ggggaaaagg ggagagcccc gcaaccgtcc
     2041 gccattcccg gtgcagaaag gatacctgga catgcctact gtcattaata atgtagagac
     2101 attatcggct gtcgtgaaga tcatggtgaa aggcgccgac tggttcaaag ccatgggcac
     2161 gaaagaatcg gccggcacca aactgctcag tgtcgccggc gactgcaaac atcccggggt
     2221 ctatgagatc gagtggggaa ccaccatccg tgaacttctg gaaatggtcg gagctacatc
     2281 ggtacaggct gttcaggtgg gcggaccatc cggggtgtgc attaatccga cccagttcca
     2341 tcgtgaaatt gcttttgaag acctggccac cggcggctcg atgatcatca tcggcaaaca
     2401 gcgtaacctg ctgaaagaag ttgtcgtgaa ttttatggac tttttcattg aggaatcatg
     2461 cagttcgtgc gttccttgcc gctcacttac ggtcatcctg aagaataaac ttcagaagat
     2521 cctcgacggc aaaggttccc ggctggatgt caatgaactt tacgaatgga ctaaatacat
     2581 gaaagcggcg aaccgctgtg gtctcggaca gaccgccgcc aacccggtac tttccaccat
     2641 cgagaatttc cgccagttgt atgatgacct ggtcatcccg gaagaggaat tcgtttccac
     2701 tttcaacctg gaagccgctg tggccgattc ctgcgcttat gtaggcagaa tcccgaaatt
     2761 cgaacatcac tgagtcagca atcattaaag aaattggaat atgagcgaga aaatcagatt
     2821 catcatagac gataaagtct gttacgccaa accgggccag actataatgg atgccgcccg
     2881 cgataatggc atttttattc cttccctttg ctacctggaa gggatcaaac cggcaggttc
     2941 atgccggatt tgcaatgtga aggtcaatgg ccggaatatg acggcctgca caaccccggt
     3001 cgccgaagga atggtcgtgg aaaacaatct cccagacctg gaagatatgc ggaaagccat
     3061 cgtggaagtt ttgttcgtag ccggcaacca tttctgccct tcttgcgaaa aaagcgggaa
     3121 ctgcgaattg caggctctcg gataccgcta caaaatgatg gtgccgcgat tcccttacga
     3181 gttcccggtg aagggtgtca atgccaaagc aaagtatatc tatcacgaca ggaaccggtg
     3241 tattctgtgt aagcgctgta tccgaaccgt tatgaaagat gataagcccg tatttgcttt
     3301 caatcagaga ggcggccatc acctgcatat cgagatggat attgaactgg ccaatagcct
     3361 gacagaagcc gaagccctgc atgcaatgga tatttgcccg gttggagcca tcatcaaaaa
     3421 agagagaggg tattattccc cgatcggatc gagaaagtat gatttcgatg aaatcggagc
     3481 tgacctggtt aaaaaagtat aagtaaaaca tttaatacgt ctgaatatga gcaaagcaat
     3541 tgtagcaacg acatccatgg ccggctgttt tggctgtcat atgtcgatcc ttgatataga
     3601 cgaacgtatc ctcgatctga tcgaactggt cgaattcaat aaatcgccga tcgatgatat
     3661 taaaaagttc accaagcagt gtgatgtggg aatcctggag gggggatgct gcaacagtga
     3721 gaatgtcgag gttctgaaag agttccgcaa aaactgcaaa atcctgatct ccgtcggtga
     3781 gtgcgccatt atgggcggat tgccggccct gcgcaacggc ataccgatcc aggaatgcat
     3841 cgaagaggct tatttgaatg ggcctactgt ggggctgaac aaggacagga tccttccaaa
     3901 tgacgatgaa ctgccgatgc ttctcgacaa ggtctatccc tgccatgaaa ttgtgaaggt
     3961 agattatttt ctcccgggat gcccaccgag ggccgacctg atctggaatg ccctggttgc
     4021 cttgctgacc ggcgacgaaa tgaagctgcc ttatgaagtt gttaaatttg attaatttgg
     4081 aggattagga catgacaaag aaaatcacga tagaacccgt caccagggtg gaagggcacg
     4141 gaaaagtcac catccacctg gatgagcaga acaatgtcgc ccaaacccgg ttacatattg
     4201 tcgaatttcg gggatttgaa cgtttcgtgc agggcaggcc ctactgggaa gccccggtgc
     4261 tggttcagcg actctgcggt atctgccccg taagccatca cctggccgct gccaaagcac
     4321 tcgatgtgat cgtcggagcc ggcaccggcg aaggacttac cccgaccggc gagaagatgc
     4381 gccgcctgat gcactacggg cagatgttcc aatcgcattc actgcatttc ttccacctgg
     4441 tttctcccga cctgctgctg ggtattgacg ccgatccggc tatccggaat gtcatcggcg
     4501 tggccattca tcatagagat ctggccgtac agggagtgat gatgcgtaaa ttcggacagg
     4561 agatcatcgc tgctacagcc ggaaagaaaa tccatggtac cggagccatc cccggaggaa
     4621 ttaacaaaca cctgaccatt gaagagcggg ataaattcct taaaggagcc gatcctctca
     4681 atatcgataa gatgatcgaa tggtcaatcg gagccctcga cttctttaaa tcctactata
     4741 aaaagaacaa ggaattcatc gattcattcg cctatttccc gtcgaaccac ctgagccttg
     4801 tccggaaaga cggagccctc gatctctatc atggtgtttt acgtgccgtg gatacggatg
     4861 gaaagaaaat ccttaatgat attgattatc agtattatgc tgattatatt gaagaagagg
     4921 tcaggtcctg gagttacatg aagttccctt atctgaagga gcttggcccg caaaaaggat
     4981 ggtacagggt aggacccctc gctcgcctga acacctgtga ctttattccg acacctctgg
     5041 ctcagaagga atttgaagaa ttcaaatcgc tgaccaacgg taaaccgaac aaccattgca
     5101 tgcattatca ttatgcacgc ctgatcgaaa tcctgcattc tgccgaggaa atcaagaaat
     5161 tgctcaacga tcccgacctg ctggaagggc cgctggttgc aaaaggccag aagctgaacg
     5221 aaggggtggg cctcatcgaa gctccccgcg gaacactctt ccatcattac cggatcaatg
     5281 aattcgacca gatcaccatg gctaacctga tcgtttccac aaccaacaac aacgagccga
     5341 tgaaccagtc ggtcaatcgc gttgccaaat cgtggatgaa cggaaagacc gagatcaccg
     5401 agccgatgat gaatgcggtg gaagtagcta tccgcgctta tgacccgtgc ctgagctgcg
     5461 caacgcatgc cctgggccag atgccactgg atatttcgct gtatgacaag gataataacc
     5521 tgatcgacag gaaacggaca taattttggc agagttcttt aacattctca tatacggata
     5581 cggaaatccc ggacgggaag acgacgggct gggtattgaa ctggtaaagc aacttgaaca
     5641 atgggctgct gagaatgaac tgcaaggaat ttctttcgat tccaactacc agttgaatat
     5701 tgaagatgct gaagctattt ccgatatgga tgtcgttatt tttgccgacg cttctacaga
     5761 agatattgag gatttcatcc tgacgaaggt aacgggggag actgaagttt catttacgac
     5821 acacgcagcc tctcccggct acattgtcaa gctttgcgag gaaatcttcg gaaaatcccc
     5881 ggaagtttat cttttgcata ttaaagggta ccaatgggat ttctatgaag gattatccga
     5941 taatgccgtt aaaaaccttg aaaatgcgtt gaattttatc agtaatttta tattagaaaa
     6001 acttaaacag aatagcaaac cttaaaacct ctctaattat ggatttgtca acatcctata
     6061 tgggattaaa gctgcggaat cccttaattg tcagcagttc acgaattacc agcacagtcg
     6121 atgatgtcaa aaaatgtgct gacaacggag caggtgctat cgtcttgaag tccttgtttg
     6181 aggaacaatt tcttgctgat tccaaccgct tgatggacca ggatcagaag tacttctggt
     6241 ttccggaagc cattgatttt atcaatcagc attccaggga acatgggatc aaaggatacc
     6301 tgaaactgat caaaggagcc aaagccaata ctgatgttcc gatcattgcc agtgtga
//
LOCUS       DAOPNH010000200         6310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14201_length_6310_cov_4.999520, whole genome shotgun sequence.
ACCESSION   DAOPNH010000200 DAOPNH010000000
VERSION     DAOPNH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14201_length_6310_cov_4.999520"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKZ74_10445"
     CDS             complement(<1..585)
                     /locus_tag="PKZ74_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM93219.1"
                     /translation="MTKQNKAYLLAGTAVFMWSTVGTAFKLTLSSLHPVIMLLYASLV
                     SIIVLFTILLIQGKLSLLLQVSRKDLLRSALNALLNPLLFYVVLFFSYDMLLTQEAMV
                     INFTWPLPLTLLSILILKQNISLKSVLAILISFLGIVVIATKGDILALHFENPAGVLL
                     ALSSTIIWSFFWIVNIRDKRDEVIKLFLNFSFGSI"
     gene            685..1629
                     /gene="arcC"
                     /locus_tag="PKZ74_10450"
     CDS             685..1629
                     /gene="arcC"
                     /locus_tag="PKZ74_10450"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPM93220.1"
                     /translation="MKKLGVVAFGGNALILGKQKGTIEEQEANAYQTCERLLVLIDNG
                     YDLVITHGNGPQVGNIMLANAAGEKLYGVPEMPLDISVAYSQGFIGYVIEQQLRNVLR
                     ENDIDRDIITIITQVLVNKDDPAFQNPVKPIGPFYTKEDAEKIRQASETIFAEDPRGR
                     GFRKVVASPRPLVIYNTKTIERIAREGQIVIAVGGGGIPAYYKTEYKLQGIDAVIDKD
                     HASALLASQIGAYRFFILTDVPKVCLNFNTPQEKTISRMNIAEAKRYLAEGHFSEGSM
                     APKIRAAIYFVEHSGRDAIITQTTLLGVDDGGTRITIV"
     gene            1728..2729
                     /gene="argF"
                     /locus_tag="PKZ74_10455"
     CDS             1728..2729
                     /gene="argF"
                     /locus_tag="PKZ74_10455"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPM93221.1"
                     /translation="MSFNLRNRSFLKLLDFTPQEIRFLLDLSAELKRSKYAGSEQQRL
                     KGKNIVLLFEKDSTRTRCAFEVAAFDQGANVTYLGPSGSQMKKKESIKDTARVLGRMY
                     DGIEYRGYGQDIVEELAKYSNVPVWNGLTNEFHPTQVLADLLTMMEHSNKPLKEIAYC
                     YLGDARNNMGNSLLVGAAKMGMDFRSIAPKEFQPAKELVDKCLEIAKETGARITVTDN
                     VAEGVKGVDFVYTDVWVSMGEPDSAWKERLALMLPYQVNSQLMAKTGNPDVKFLHCLP
                     SFHSRETTVGEEIFQKFGVAEMEVTDEVFESPASIVFDQAENRLHTIKAVMVATLGS"
     gene            complement(2740..3366)
                     /locus_tag="PKZ74_10460"
     CDS             complement(2740..3366)
                     /locus_tag="PKZ74_10460"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPM93222.1"
                     /translation="MKPLPHKTIKRLSQYRRALLMVHTSGKTHIFSHEIARIIHITAV
                     QVRRDIMLIGYTGTLRKGYNVKELIDLIGEIIDSESIIKVCVIGVGNLGRAIINYFSG
                     KRTKLSIVAVFDSNIEKVGKKYSGVTCYDIGQIDEIVKKEDIKVGIITVPADQGQITA
                     DRLVKAGIRGILNYAPKSVSVPKDVHLEEYDMITSLEKVAFFVKTHNL"
     gene            complement(3459..4244)
                     /gene="hypB"
                     /locus_tag="PKZ74_10465"
     CDS             complement(3459..4244)
                     /gene="hypB"
                     /locus_tag="PKZ74_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPM93223.1"
                     /translation="MCGTCGCGQPDPISYKKPGEAGSIKYNHHHNHTHAHDHDHPGHE
                     PSDQSREISLEIDIMLKNNLLAENNRGYFEGRKISALNMVSSPGSGKTAILERTIKEY
                     GSHRKVFIIEGDQQTMLDAERIRKAGAPVIQVNTGNGCHLDARMVRTAADKLEISDGS
                     LLFIENVGNLVCPALFDLGESKRVVIISVTEGDDKPLKYPNMFQTSHLCLINKSDLLP
                     YVDFNLDKCKEYALKVNPALQFLTVSAQTGEGFPGWIDWIKQI"
     gene            4383..4793
                     /locus_tag="PKZ74_10470"
     CDS             4383..4793
                     /locus_tag="PKZ74_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93224.1"
                     /translation="MTKVVSLDVKCPHCDASFMDEGHLINNKASIKLLIHTPIGQKGF
                     IWLSSIYGDYNFISEFQIKEGEVVEFFCPVCKESLKRKKIECDVCNAPIVSLNCTIGG
                     RVNICSRSGCKNHYIVFEDLDTTIRKFYEDYGYH"
     gene            complement(4815..5837)
                     /gene="hypE"
                     /locus_tag="PKZ74_10475"
     CDS             complement(4815..5837)
                     /gene="hypE"
                     /locus_tag="PKZ74_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPM93225.1"
                     /translation="MMLHNDTILLGHGSGGKLSHDLIGDLFVRHFHNIHLEKQTDSSI
                     LNIESGKIAFTTDSFVVDPIFFPGGDIGKLAVCGTVNDLAVSGARPLYLSAGFIIEEG
                     FLYTDLEKIVVSMADEARKAGVMIVTGDTKVVDRGKCDKVFINTSGIGILSERYSHIS
                     DGSKIRPGDKVIINGPVGEHGMAVLSARKDLNIKSSIVSDCASLNRMIAGVLEATDGV
                     RFMRDATRGGLATVLAELAEKNQLGISIEEEKLPVTSQVRSFCELLGFDPLYVANEGK
                     VVIISSKESADQVILSLKKSEYGKGAEIIGEICGDYPGKAYLNTIVGGRRIIDMLAGE
                     QLPRIC"
     gene            complement(5845..>6310)
                     /gene="hypD"
                     /locus_tag="PKZ74_10480"
     CDS             complement(5845..>6310)
                     /gene="hypD"
                     /locus_tag="PKZ74_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPM93226.1"
                     /translation="VAERYRLGCVIAGFEPVDLMESILMLVRQIEESSQRIEIQYKRA
                     VPAEGNPQARKLMDQVFELRDEWWRGLGILPLSGLKLKNQYSRYDAEKVFGVEPEPTK
                     EDKGCICGEILKGLKTPKECKLFGTACTPVNPAGPCMVSNEGACHAFFRYNR"
BASE COUNT         1717 a         1449 c         1465 g         1679 t
ORIGIN      
        1 gatagatcca aaagagaagt tcaggaataa ttttatcacc tcatcccgtt tatcgcggat
       61 attcacaatc cagaaaaaag accagataat tgtgctgcta agagccagca atacgccggc
      121 cggattttca aagtgaagag cgagaatatc cccttttgtc gcgataacaa caatccccag
      181 gaagctgatg aggatggcca gtacactttt aagactgata ttttgcttca ggattaggat
      241 tgataataag gtaagaggca gcggccaggt gaagttgatt accatggcct cctgggtaag
      301 caacatatcg taggagaaga aaagaacaac ataaaacagc agcggattga gcaaggcatt
      361 cagggcggaa cggagcaggt ctttgcgtga aacctggagg agaaggctca attttccctg
      421 gatcagaagg atggtaaaca gaacgattat cgaaaccagc gaggcataca gaagcataat
      481 taccggatgc agcgacgaca gggtcagctt aaaggcagtg cccactgtcg accacatgaa
      541 tacggccgtg ccagccaata gatatgcttt gttctgcttt gtcatccggc gtattagaac
      601 aggagcgaag ataaatattt agctgaaaga tacagaatga gcctccaaat attattagct
      661 ttgcagcctt aaacggtatt ttatatgaaa aagcttggtg tggttgcttt cgggggaaat
      721 gccctgattt tgggaaagca gaaaggaact attgaagaac aggaagccaa tgcatatcaa
      781 acctgtgaac gcttgctggt acttatcgat aacgggtatg acctggtgat tacccatggc
      841 aacgggccgc aggtaggcaa tattatgctg gccaatgctg ccggtgagaa actgtacggt
      901 gttcctgaaa tgcctctgga tatcagtgtt gcctattccc agggctttat cgggtatgta
      961 atcgaacaac agctcaggaa tgtattgcgc gaaaacgata tcgaccggga tatcattact
     1021 attatcacac aagttcttgt aaataaagat gatccggcct ttcagaatcc ggttaagccg
     1081 atcgggccat tctatacaaa ggaggacgcg gagaagatca gacaggcttc cgaaaccatt
     1141 tttgccgagg atccgagggg caggggattc cggaaagtgg ttgcttcacc caggcccctt
     1201 gtgatttaca atacgaaaac catcgaacgg attgccaggg aaggccagat cgttattgcc
     1261 gtcggaggtg ggggaatacc tgcctattac aagacagaat acaagcttca gggcatcgat
     1321 gccgttatcg ataaagatca cgcatccgca ctgctggctt cgcaaattgg cgcttaccgg
     1381 ttctttatcc tgaccgatgt acccaaagtc tgccttaatt tcaatacacc ccaggaaaaa
     1441 acgatcagcc ggatgaacat tgcagaagcg aaacgttacc tggcggaagg gcatttttcg
     1501 gaaggcagca tggcgccgaa gatcagggca gccatctatt ttgtcgaaca cagcggccgc
     1561 gatgccatca tcacccaaac cactctcctg ggtgtcgatg acggcggcac aaggattaca
     1621 attgtctaat aaacaagtga ttagatacga tttccgattg aaaattaata ttgaaaattg
     1681 aaaattaaga atttagaatt aaaaatttag aactaaaatt atatcgtatg tcattcaatt
     1741 tgcgcaacag gagcttttta aagctattgg attttactcc tcaggaaatc aggtttttat
     1801 tggatctatc ggcagaactg aaacggtcaa aatatgccgg gagcgagcaa caaaggctga
     1861 aaggtaaaaa cattgtcctg ctttttgaaa aagactccac acgcacccgc tgtgccttcg
     1921 aagtggccgc atttgaccag ggagcgaatg tgacttatct cggcccatcc ggttcacaga
     1981 tgaagaaaaa agagtcgatc aaggataccg ccagggtact cggtcgcatg tacgacggaa
     2041 ttgaataccg gggatacggc caggatatcg tcgaggaact ggcaaaatat tccaacgtcc
     2101 cggtgtggaa cggcctgacc aatgaattcc atcctacaca ggtacttgcc gatttgttga
     2161 cgatgatgga gcacagcaat aagccattga aagagatcgc ttattgttac ctcggcgatg
     2221 ccaggaataa tatgggcaat tcgctcctgg tcggcgcagc taaaatgggt atggatttcc
     2281 gttccatagc acccaaagag ttccagccgg ccaaagagct ggtcgataaa tgcctggaaa
     2341 tagccaaaga gaccggcgcc cgcatcacgg tgaccgataa cgtggccgaa ggggtaaaag
     2401 gggtcgattt tgtttatact gatgtctggg tttcgatggg cgaacccgat tctgcctgga
     2461 aagaaagact ggcactcatg ctgccttacc aggtgaacag tcagcttatg gctaaaacag
     2521 gcaatcctga tgtcaagttt ttgcactgcc tgccctcttt ccatagccgt gaaactacag
     2581 taggagagga gatattccag aaattcggcg ttgctgaaat ggaagtgacc gacgaagtct
     2641 tcgaatcacc ggcatccata gtgtttgacc aggccgaaaa ccgcctgcat accatcaaag
     2701 ccgtaatggt ggctacgctg ggaagttaaa cacagtagtt taaagattgt gcgttttaac
     2761 aaagaaggcg actttctcca gtgaggttat catatcatat tcctccaggt gaacgtcttt
     2821 aggtacagaa actgattttg gtgcataatt cagaatacct ctgatacctg ccttgaccag
     2881 tcgatcggct gtaatttgcc cctggtcggc gggaacggta ataattccga ctttgatatc
     2941 ttctttttta acgatctcgt cgatctgacc gatatcatag caagtgaccc ctgaatattt
     3001 cttgcctacc ttttcaatat tgctgtcgaa tacggctaca attgaaagct tcgtcctttt
     3061 tccgctgaag taattgatga tggctcttcc gaggttcccg acgccgatga cacatacctt
     3121 aataatgctc tcactgtcga tgatttcacc aatgagatcg atcagttcct tgacgttata
     3181 gcctttgcgc agggtgccgg tatatccgat cagcatgata tccctcctga cctgcacagc
     3241 cgtgatgtga atgatcctgg caatctcatg agagaatatg tgggtcttgc cactggtatg
     3301 caccatgagt aatgcccggc gatactggct cagtcgttta atcgttttat gagggagtgg
     3361 tttcatcctg atgaaattat tttcgcatta aaacacacaa aaataatttt agaagggata
     3421 aaatccaaat gttttgttaa ttatttaaca attcggtttc aaatttgttt gatccagtcg
     3481 atccaacccg ggaatccttc accggtttgg gccgatactg taaggaattg aagcgccgga
     3541 ttaactttca atgcatattc cttacattta tccagattga aatcgacata ggggagcaaa
     3601 tcggacttat tgatcaggca aagatgggat gtctggaaca tgttcgggta tttcagcggt
     3661 ttatcatccc cttcggtaac actgataatc accacgcgct tcgattcccc aagatcgaac
     3721 agggcagggc aaaccaggtt gccgacattc tcaatgaata gcagcgatcc gtctgagatt
     3781 tccagtttat cggccgctgt gcgaaccatc cgggcatcca ggtgacaacc attcccggta
     3841 ttgacctgta taacgggagc gcccgctttc cggattcgct cggcatccag catggtctgc
     3901 tggtctcctt caatgatgaa aacctttcta tgtgatccgt attcttttat ggtccgctca
     3961 agtattgccg tttttcctga tcctggcgaa ctgaccatgt tgagtgcact tatctttctg
     4021 ccttcgaaat acccccggtt attttctgct aaaaggttgt tctttaacat gatatcgatt
     4081 tccaggctga tctccctgga ttgatcagat ggctcgtgac cgggatgatc atggtcatga
     4141 gcatgggtat ggttatgatg atggttatat tttattgaac ccgcctctcc aggctttttg
     4201 taactgatcg gatctggttg tccgcatccg caagttccgc acatattctt attttttgag
     4261 caaaggtaag cattatcatc aggatagaat tgttctaaat tgcaaatttc ttactgtatt
     4321 ccaaattttg attaggaaaa atagtcaata atcaatatat ttgttttagc aaatctatga
     4381 tcatgacaaa agttgtttca ttagacgtta aatgcccgca ctgcgatgcc tccttcatgg
     4441 atgaaggtca cctgatcaat aacaaagcca gcattaagtt gttgatacat actcccatcg
     4501 gacaaaaggg atttatttgg ctaagctcaa tttatggtga ttataacttt atttcagagt
     4561 tccagatcaa ggaaggggag gtcgttgaat tcttctgccc tgtttgcaaa gaaagcctaa
     4621 agaggaaaaa aatcgagtgc gatgtgtgta atgctccgat tgtcagcctg aattgcacta
     4681 tcggagggcg ggtgaatatt tgcagccgca gtggatgtaa aaaccattac atcgtttttg
     4741 aagacctgga tacaacgatc cggaagtttt atgaagatta tggataccat tgatcgctga
     4801 tcaccgggtt cggttcagca gatcctgggt aactgttcgc ccgccagcat atcgatgatc
     4861 cttcttcccc cgacgatggt gttcaaatag gccttgccgg gataatcacc gcaaatttcc
     4921 cctataattt cagccccttt gccatattct gatttcttca gtgaaagaat tacctggtcg
     4981 gcagattctt ttgatgaaat gattactact ttgccttcat tggccacata aagcggatcg
     5041 aatcccaata attcacagaa agacctgacc tgggaagtca cgggcaattt ttcctcttca
     5101 atacttatac ccagttgatt tttttctgcc agttcggcca gaacagtggc caggcctccc
     5161 cgggtggcat cacgcatgaa ccgcacaccg tcggtggctt ccagcacacc ggcaatcatc
     5221 cggttcagcg atgcgcaatc tgaaacaata ctgcttttga tattgagatc cttccgggcc
     5281 gataaaacgg ccatcccatg ctctccaacc ggcccgttaa taatgacttt gtcgccgggc
     5341 cgtattttac tcccgtcact tatgtggctg tacctctcgc tgaggatgcc tattccggag
     5401 gtgttgatga acaccttatc acatttgccc ctgtcaacta ccttcgtatc gccggttacg
     5461 atcattactc ctgcctttcg ggcttcatcg gccatggaga cgacaatctt ttcgagatct
     5521 gtataaagaa aaccctcctc gataatgaat ccggcactaa ggtaaagtgg ccgggccccg
     5581 gatacagcca ggtcgttgac cgtgccgcat acagccagtt tgccaatatc gccgcccggg
     5641 aagaaaattg gatcgacgac aaaggaatcg gtcgtgaatg cgattttccc gctttcaata
     5701 ttcaaaatac tcgaatcggt ctgcttttcc aggtgaatat tgtgaaagtg acggacaaac
     5761 aggtcaccga taaggtcatg gctcaatttc cctccgcttc cgtgaccgag taaaatagta
     5821 tcattatgca acatcattcg ggagttatct gttataacgg aaaaaagcat ggcaagctcc
     5881 ttcattggag accatgcagg ggccggccgg attgaccggg gtgcaagcgg tgccgaaaag
     5941 cttgcactct ttcggcgttt ttaatccttt caggatctcc ccgcagatac atcccttgtc
     6001 ttctttcgta ggttccggtt ccacgccgaa aactttttcg gcatcataac gggaatattg
     6061 attcttcagt ttcaaaccgc ttaatggcaa tatacccagc ccgcgccacc actcgtcgcg
     6121 aagctcaaaa acctggtcca tcaactttcg ggcctggggg ttaccttccg ccggtacggc
     6181 tcttttatac tgaatctcaa ttctttgtga ggattcctcg atttgtctga ccagcataag
     6241 gatcgattcc atcagatcga caggttcgaa tcctgcgata acacagccca atcggtatct
     6301 ttcagccaca
//
LOCUS       DAOPNH010000201         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14255_length_6292_cov_6.127786, whole genome shotgun sequence.
ACCESSION   DAOPNH010000201 DAOPNH010000000
VERSION     DAOPNH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14255_length_6292_cov_6.127786"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PKZ74_10485"
     CDS             complement(<1..944)
                     /locus_tag="PKZ74_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPM93227.1"
                     /translation="MAIIDLVRWAPQGDQTIYAFRFPETNLSTYTQLIVQESQEAILF
                     SKGQIVGKFGPGKHTLSTENLPILRNLFGLPFGGKNPFTAEIWFINKLQPYNIDWQID
                     RMDIHDADYNTGIPLVAKGRYGLRINDSERFLVKIVGPKESFDEFDLTDQFSGEFHTK
                     TKSTIMQFMLNNGIGLKKISAFLDNISEHLRDIMLPFWENLGFELTRFYVTSIEVDGT
                     TEVGKRILDALSRQSAQAIGGYTWQQSQAFEVAKDAVDGLSNSSNGLLGAVIASNMMG
                     GLGGGGGMMNPQYNQPTFGPAGQGQQGPQAGQPAAQIK"
     gene            1217..>2546
                     /locus_tag="PKZ74_10490"
     CDS             1217..>2546
                     /locus_tag="PKZ74_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPM93228.1"
                     /translation="MVFSSTLFLLYFLPVFLGIYFLLPNKLRNLWLLVASVLFYSWGA
                     PDFIFIVLGSILADFYLVKYIHRAGLKRKRWLTAISIVMNVGLLAYFKYANFFVDNLN
                     SLLTGLGMTQVHWTAIALPIGISFFTFQKITYTVDVYRNVHAPLKKITDYAMYILMFP
                     HLIAGPIVRFNLVADQIEDRRYNETIGNRVNGLFRFVIGLSKKVLIANVLGAEADKIF
                     AMDPSTFGAAQAWTGIIAYAFQIYFDFAGYSDMAIGLALMIGFNFPENFNNPYISQNI
                     TEFWRRWHMTLGRFMREYLYIPLGGNRVGTARLYLNLWIVFFLSGLWHGAAWNFVAWG
                     AFHGLFLVADRVFLIKWLTKAGKYPAVAITFFISLIGWVLFRSETLGYAMRFLRSMFT
                     FGSDFGGVTLNNKFIVILVIAVIFSFIGIFKGNEAWQERILRGRGDGEMGR"
     assembly_gap    2547..2646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2647..4436
                     /locus_tag="PKZ74_10495"
     CDS             <2647..4436
                     /locus_tag="PKZ74_10495"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93229.1"
                     /translation="HLFQILMKKALPHILFAVILLIALLPAVQMRWSLIEEKPLNGAF
                     ELAEKPVLSYENWMSGDFQMQTEKYLKDHSGFRNFLIRLQNQLDFSLFKQANAEGATV
                     GKKGQLFEYDYIRSWLALDYPGDSFLEKKLQRARFVQDYLKREKGIDLVVVFEPGKAS
                     YYPEYLPSSYKKQKKGPSTYDYFVKKAKETGIDYIDLQQYFLELKDTSRYPLFPAYGT
                     HWSVYGMKFAADSLLRLIGNRRNLDLTDVRLTGIETSTRPRDTDDDVLKTMNLLCPMQ
                     GEELAYPLLAFDTLNPGEKPMVLVVADSYYWNIFNTRIPKYVFANEAFWYFNALVYPD
                     HYNRPTFTKDLDLKAEIEKQDIIFLMITERFVHKFDWRFTDQLYELYAPQGLSDPVYD
                     KINNIMTVEEWFEKMITKAGYKHATLEEILIDEGKYLYHMSDTAGFMIRYGEEYFRKM
                     ISGDRKWMGHIEEKAARRGIPVEDMLVEDALFVFKEKHPELYKLNRSLESVRKHILAN
                     PDTLALLREEARNYHFDPDLYLQHRAWEIVHRDEVAKTERAIRQYAPWLEDVARKALE
                     NGISVDEMIRLDAEYMWKERLRSVGFKEK"
     gene            4484..5050
                     /gene="efp"
                     /locus_tag="PKZ74_10500"
     CDS             4484..5050
                     /gene="efp"
                     /locus_tag="PKZ74_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPM93230.1"
                     /translation="MPTTADFRNGLCIDFNNDLWSIVEFQHVKPGKGGAFVRTKLKNL
                     RDGRVLENTFIAGVKINIVRIERRPHQYLYNDGESYYFMNADTFEQVFVEKKLLEAAD
                     LLKEGVTVDLIFHSETETVLQCEMPTTVTLEVTYTEPGEKGNTATNTLKEAKVETGAL
                     VRVPLFINTGDRIKVDTRTYRYAERAKD"
     gene            5055..5996
                     /locus_tag="PKZ74_10505"
     CDS             5055..5996
                     /locus_tag="PKZ74_10505"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPM93231.1"
                     /translation="MIFSRPYTLKEIADLLGAKYDGDPSFEITGINEIHMVEPGDLTF
                     VDHPKYYDKALNSKATTILINKEVACPEGKALIISDDPFRDYVWLAKYFRPFVASSAA
                     ISPTAEIGEGTVIQPGVFIGNHVKIGKNCIIHSNVSIYDHSIIGDEVIIHANSVIGAD
                     AYYFQKKPAGYRKMESCGRVVIKDRVEIGALCSIDKGVSADTIIDEGTKFDNHVQVGH
                     DTYIGKHCLIGAHCAIAGVTKIEDEVILWGRVAVNKDLVIGKGAVILATSAVDKSIEG
                     GGKTYFGAPAEEARKKWREMVYLRQLPELFEKVLGKQ"
BASE COUNT         1630 a         1548 c         1473 g         1541 t
ORIGIN      
        1 tctttgatct gcgcagcagg ctggccagcc tgagggccct gctgcccctg ccctgccggc
       61 ccaaaagtcg gctggttgta ttgaggattc atcatcccgc caccaccgcc aaggcctccc
      121 atcatgttgg atgcgatgac tgctcccagc aatccgttac tgctgtttga gagtccgtca
      181 acggcgtctt tggccacttc aaatgcctgc gattgctgcc aggtatatcc gccaatggcc
      241 tgggcactct gccggcttaa agcatccagg attctctttc ccacttcggt tgttccgtcg
      301 acttcaatgc tggtaacata aaaacgggtc agttcaaagc caagattttc ccagaaagga
      361 agcatgatgt cacgcaagtg ctctgaaata ttatctagaa aggcagaaat cttcttcagt
      421 ccaataccat tattcagcat aaattgcatt attgtggatt ttgtctttgt gtgaaattca
      481 ccggaaaact ggtcggtcag gtcaaattca tcgaatgact ctttcggacc tacaattttc
      541 accaggaacc gctccgaatc attgattctc agtccatatc ttccttttgc cacaagagga
      601 attccggtgt tataatcggc atcatgaata tccatacggt caatctgcca atcgatatta
      661 tacggttgta acttgttgat aaaccatatt tctgccgtga aaggattttt ccctccaaaa
      721 ggtaaaccga ataaatttct cagaatcgga aggttttccg tgcttagggt atgtttacct
      781 ggtccaaact tacctacaat ctgccccttt gagaacaaaa tagcctcttg cgattcctgg
      841 acgatcaatt gagtataagt actcagattt gtttcgggaa accggaatgc gtaaatcgtt
      901 tgatctccct gtggtgccca tcttactaaa tctataattg ccataaataa taccttgttt
      961 attaatgaat taaataatta gatcacgata ataatatttt aaaccgaaat agaataagaa
     1021 actatgacaa gcgataaaac cataataatg gactcttttg atatggcgga ttaaagttat
     1081 caggaaaatt gcttaattcc aacgttaata aaaacttttt tttgagatgt caggatgatg
     1141 attctatatt accgaaaaac ataatattgc aaaaaattcg attgcaatca gatgtaaatc
     1201 tttccaaaca gagtaaatgg ttttcagcag caccctattc cttctttatt tcctgccggt
     1261 tttcctgggc atctacttct tgctgcccaa caaactgagg aacctgtggc tgctcgtcgc
     1321 cagcgtcctc ttttattcct ggggcgcccc cgattttatc tttatcgtcc tcggatcgat
     1381 cctggccgat ttctacctgg taaaatatat ccaccgggcc ggcctgaagc ggaaaagatg
     1441 gctcaccgcc atctccatcg tgatgaatgt cgggctgctg gcttatttca aatacgccaa
     1501 tttctttgtc gataacctga acagtctgct tacgggactg gggatgacgc aggtgcactg
     1561 gaccgctatc gccctgccaa tcggaatttc tttcttcacc tttcagaaaa tcacctatac
     1621 tgtagatgtt taccggaatg tccacgcgcc actgaaaaag attaccgatt acgccatgta
     1681 catcctgatg ttcccccacc tgatcgcggg gccgatcgtc aggttcaacc tggtcgccga
     1741 ccagatcgaa gaccggcgtt ataatgaaac gatcgggaac cgggttaacg ggctgttccg
     1801 ctttgtgatc gggctgtcga agaaggtgct gatagccaat gtcctgggcg ctgaagccga
     1861 caaaatcttt gccatggacc cctccacgtt cggcgcggca caggcctgga ccggcatcat
     1921 cgcctatgct ttccagatct atttcgactt tgccggctac tccgatatgg cgatcggcct
     1981 ggccctcatg atcggtttca atttccccga aaatttcaat aatccgtata tctcacagaa
     2041 tatcaccgag ttctggcgcc gctggcatat gacgctcggc cggtttatgc gggaatacct
     2101 gtatatcccg ctgggcggca atcgtgtcgg aactgcccgc ctctacctga acctctggat
     2161 cgtattcttc ctttccggcc tctggcacgg agccgcgtgg aatttcgtgg cctggggcgc
     2221 ttttcacggc ctcttcctgg tagccgaccg ggtcttcctg atcaaatggc tcacgaaagc
     2281 aggcaaatac cccgctgtag ccatcacttt cttcatctcc ctgatcggat gggtcctgtt
     2341 cagatcagaa accctcggtt atgccatgag attcctgcgg tcgatgttca ccttcggcag
     2401 cgatttcgga ggtgtaacgc tgaacaacaa gttcatcgtg atccttgtca ttgctgttat
     2461 cttttcgttc atcggtattt tcaaaggaaa tgaagcctgg caggagcgga tcttgagagg
     2521 acggggagat ggggagatgg ggagatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnttca tttatttcag attctgatga agaaggcatt accccatatc ctttttgcgg
     2701 tgatcctcct gatcgctttg ctgccggcgg tgcagatgag atggagtctc atagaggaaa
     2761 aaccgctcaa cggcgccttc gaactggccg aaaaacctgt cctgtcttat gaaaactgga
     2821 tgtcgggcga tttccagatg caaactgaaa aatacctgaa agaccattcc ggcttccgga
     2881 actttttaat aagattgcaa aaccagcttg acttctccct gttcaaacaa gccaatgcgg
     2941 aaggggccac tgtcggcaaa aaagggcagc tcttcgaata tgattacatc cgttcctggc
     3001 tggcgctgga ttatcccggc gattcgttcc tggaaaagaa gctgcagcgg gcccgttttg
     3061 tccaggatta cctgaaaagg gaaaaaggga tcgacctggt cgtagtcttc gaaccgggca
     3121 aagcctccta ctatcccgaa tatcttccat catcttacaa aaaacaaaaa aaaggcccct
     3181 ctacctacga ttactttgtg aaaaaagcaa aggagacggg gattgattat atcgacctgc
     3241 agcagtattt cctggaactg aaagatacca gccggtaccc gctatttccc gcatacggca
     3301 cacactggag cgtctatggg atgaaatttg ccgccgacag ccttctccgt ctgatcggga
     3361 accggagaaa cctggacctg accgatgtca ggttaaccgg tatcgaaacc tcgaccagac
     3421 caagggatac agacgatgat gtactgaaaa cgatgaacct cctctgcccg atgcagggcg
     3481 aagaactggc ctatcccctc ctggcgttcg atacgctgaa tcccggcgaa aaaccgatgg
     3541 tgctggtggt ggccgacagt tattactgga acatcttcaa tacccggatc ccgaagtatg
     3601 tttttgccaa cgaagcgttc tggtacttca acgccctggt ttaccccgat cattacaaca
     3661 ggccaacctt tacgaaagat ctggacctca aagcggaaat tgaaaagcag gacataatct
     3721 tcctgatgat caccgagagg ttcgtgcaca aattcgactg gcggttcacc gaccagcttt
     3781 atgagcttta tgcaccgcaa gggctctccg atccggtgta cgacaagatc aacaacatta
     3841 tgacggttga ggagtggttt gaaaaaatga tcaccaaagc gggatataag catgcgacac
     3901 tggaagagat cctgatcgat gaagggaaat acctttatca tatgtcggat acggccggtt
     3961 tcatgatccg gtatggcgaa gaatatttcc gcaagatgat ctccggtgac cggaaatgga
     4021 tggggcatat cgaagaaaag gcggcccggc ggggcattcc ggtggaggat atgctggtag
     4081 aggatgcttt gtttgttttt aaagaaaagc atccggaatt gtataagctg aaccgcagcc
     4141 ttgaatccgt gagaaaacac attttagcca atcccgacac cctggcctta ctacgcgaag
     4201 aagcccggaa ttaccatttt gatcccgatc tctatcttca gcacagggcc tgggaaatcg
     4261 ttcaccggga tgaagtggcc aaaacggaaa gggccatccg gcaatatgcc ccctggctgg
     4321 aggacgtggc acgcaaggca ctggaaaacg ggatcagtgt cgacgaaatg atccggctgg
     4381 atgccgaata tatgtggaaa gagcgattac gatcggttgg ttttaaggaa aaatagttta
     4441 cttttgcaca aaatttcaaa atcctttact aaatccagat tatatgccta ctaccgctga
     4501 tttcaggaat ggcttgtgca tcgatttcaa taatgacctc tggtcgatcg ttgagtttca
     4561 acatgtgaaa cccggcaagg gaggcgcgtt tgtgagaacg aagctgaaaa acctccgcga
     4621 cggcagggtg ctcgaaaaca cctttatcgc cggtgtaaag atcaacatcg tcaggatcga
     4681 aagacgtcct caccagtatc tctacaatga cggcgaatcc tactatttca tgaatgccga
     4741 caccttcgag caggtgtttg tcgagaagaa actgctggaa gccgccgatc tcctgaaaga
     4801 aggggtaacg gtcgatttga tcttccattc cgaaacggaa acggttttgc aatgtgaaat
     4861 gcccactacg gttacgctcg aagtgaccta taccgaaccg ggagaaaaag ggaatacagc
     4921 cactaacacc ctgaaggaag ccaaggtgga aaccggcgcc ctggtaagag tccccctgtt
     4981 catcaatacc ggcgaccgga tcaaggtcga tacccgtacc taccgctacg ctgaacgggc
     5041 aaaagactga taatatgatc ttctcccgtc cctataccct gaaagaaatt gccgatctgc
     5101 tcggcgcaaa gtacgatggc gatccgtcgt tcgaaattac cgggatcaac gagatccaca
     5161 tggtggagcc gggcgatctg acctttgtcg accaccccaa gtattatgat aaagcgttga
     5221 attccaaagc caccaccatc ctgatcaaca aagaggtagc atgcccggaa ggaaaagcgc
     5281 tgatcatctc cgatgatccc ttccgcgatt atgtctggct agcgaaatat ttcaggccgt
     5341 ttgtggcatc atccgcagcc ataagcccca ctgccgaaat cggcgagggt acggtcattc
     5401 agcccggagt ctttatcggg aaccacgtaa agatcggaaa gaactgtatc atccattcca
     5461 atgtcagcat ttacgatcac agtatcatcg gcgatgaggt gatcatccat gctaacagtg
     5521 ttatcggggc cgatgcctat tattttcaga agaagccggc cggttaccgt aaaatggagt
     5581 cgtgcggccg ggtagtgatc aaagaccggg tggagatcgg cgccctgtgt tccatcgaca
     5641 aaggtgtatc ggccgatacg atcattgatg aggggaccaa attcgacaac catgtccagg
     5701 tcggtcacga cacctacatc ggcaagcact gcctgatcgg ggcccattgc gccatagccg
     5761 gcgtaacgaa aattgaagac gaagtgatcc tctggggacg tgttgcagtc aacaaagatc
     5821 tggtcatcgg caaaggggcc gttatcctgg ctacatcggc tgtcgacaag tccattgaag
     5881 gcggcgggaa aacctatttc ggagcgccgg ccgaggaagc ccgcaaaaaa tggcgcgaga
     5941 tggtctacct gaggcagttg ccggaattgt tcgaaaaagt tctgggtaaa caataggcat
     6001 tctcactaaa gggctaccat actgttctcc ctacgggaga aatttgggga tttgacgatt
     6061 tggcgatttg acgatttggc gatttgagga tttacccgtt agacttccaa actaaatcga
     6121 aaatgccgaa tgcagaatcg ggtaatgtta ttatccaacg accatcatct taccacaaac
     6181 ttaccactcc ccaaaaaatc gcccccattt ctcagaaccg cgatatacac ccccggcgga
     6241 taactttcta cattgatctg tacctggtcc tgcttgccag gaacctttat ct
//
LOCUS       DAOPNH010000202         6278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14283_length_6278_cov_4.872087, whole genome shotgun sequence.
ACCESSION   DAOPNH010000202 DAOPNH010000000
VERSION     DAOPNH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14283_length_6278_cov_4.872087"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2274
                     /gene="rpoB"
                     /locus_tag="PKZ74_10510"
     CDS             <1..2274
                     /gene="rpoB"
                     /locus_tag="PKZ74_10510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPM93232.1"
                     /translation="YTHYGRLCTIETPEGPNIGLISSLCVFAKVNTLGFIETPYKRVE
                     KGKVLLDEAPIFLSAEEEEKKIIAQASAPLQNDGMFKNDKVKVRYQADYPIIERQDVD
                     LMDTAPNQIASIAASLIPFLEHDDANRALMGSNMMRQAVPLMEPEVPLVGTGIEGQVV
                     RDSRVLINAEGDGYVEYVDAKEIVIRYTRDEMEKLVSFDEDIKKYELTKFLRTNQNTC
                     INLRPIVRKGEKVTKGQVLCEGYATKNGELALGRNLMVAFMPWKGYNFEDAVVINEKV
                     VKEDIFTSIHIEEFTLEVRDTKRGIEELTNDIPNVSAEATKDLDENGLIRIGAEVKEG
                     DILIGKITPKGESDPTPEEKLLRAIFGDKAGDVKNASLKAPPAMKGVVIENKLFSRII
                     KDRKSRSKEKDVLKDLDDQMHKEVNDLKSKLVDKLMILVSGKTSQGVYNNFKEELVPK
                     KAKFTQKMLIGLDYASINPNKWTSDKDKNDLIKVLINNYNIKHKELLGRFNRMKFQST
                     VGDDLPNGIVQMAKVYVARKRKLQVGDKMSGRHGNKGIVARIVRQEDMPFLEDGTPVD
                     IVLNPLGVPSRMNLGQIYETVLGWAGYKLGETYGTPIFDGASYQQINEKMEAAGLPKD
                     GKVYLYDGETGERFHQPTTVGFIYMLKLHHMVEDKIHARSIGPYSLITQQPLGGKAQF
                     GGQRFGEMEVWALEAFGASNILQEILTVKSDDVQGRAKTYETIVKGENMPKPSIPESF
                     NVLVHELRGLGLEVSFE"
     gene            2304..>6278
                     /gene="rpoC"
                     /locus_tag="PKZ74_10515"
     CDS             2304..>6278
                     /gene="rpoC"
                     /locus_tag="PKZ74_10515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPM93233.1"
                     /translation="MAFRRETNTVIDFNSITISLASPESVLERSFGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIICDRCGVEVTEKKVRRERTGHIQ
                     LVVPVAHIWFFRSLPNKIGALLGLPSKKMDSIIYYEKYVVIQPGIKEKDGVKELDFLS
                     EEEYFEIVDNLPADNAHLDDSDPNKFIAKMGAEALFELLEKLDLDKVSYDLRHKANTE
                     TSQQRKQEALKRLQVFEAFRDARTRTENKPGWMILKIIPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLIEIKAPDVIMRNEKRMLQEAVDSLLDNSRKVSAVKTES
                     NRPLKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNECGLPKDMAAELFK
                     PFIIRKLLERGIVKTVKSAKKIVDRKDAVVWDILENILKGHPVMLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQLLMLASHNILNPA
                     NGAPITVPSQDMVLGLYYMTKGRKSTPEHKVKGEGMRFYSPEEVIIAFNEGKLDLHAN
                     ISVKVEDLVDGVPENQVVETTTGRVLFNQVVPSEMGYINSLLTKKALRDIIGDILKAT
                     GTKKTADFLDNIKNLGFMMAFRGGLSFNLGDVIVPKVKESFIEEANQEVEEVLSNYNM
                     GFITNNERYNQIIDIWTHTNSKLTQQVMKEISTDQGGFNPIFMMLDSGARGSKEQIRQ
                     LSGMRGLMAKPQKSGATGGEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTA
                     DAGYLTRRLVDVAQDVIISEDDCGTLRGITIAALKKNEEIVESLYDRILGRVTLHDIF
                     HPNSGELIAYAGQELTEEKCKMIEDSPIEAVEIRSVLTCESKQGVCARCYGRNLATGR
                     MVQEGEAVGVIAAQSIGEPGTQLTLRTFHVGGTASNVAIISKIEAKYDGILEIDELRF
                     VPFTNREGKSFDVVIGRSAEMRIVDKHTRIVLASGHIPYGANLYFKENTEVRKGNLIS
                     DWDPFNAVILAEVKGSIQYENIVEGKTYRIESDEQTGYVDKVVIEPRQKLKNPTINII
                     SGDGEILKAYDIPVGARIIVEDGQEIKSGEPLVKIPRAIGKTGDITGGLPRVTELFEA
                     RNPSEPAVVAEIDGVVTINKKLKRGNREVIITSKTGEEKHYLIPTTKQILAQENDYIR
                     AGTPLSEGEISPSDILAIKGPMKVQEYIVNEVQEVYRLQGVKINDKHFEVIVRQMMRK
                     VEVDDPGDTRFLEGELVNKTDFQDENDDVFGKKVVVDSGDSLSYRAGQIVSARKLRDE
                     NSMLK"
BASE COUNT         1850 a         1392 c         1596 g         1440 t
ORIGIN      
        1 tatacccatt atggccggct ctgtacgatt gaaacgcctg aaggccccaa tatcggtctt
       61 atctcttcac tttgcgtttt tgcaaaggtg aatactctcg gttttatcga aaccccttac
      121 aaacgggttg agaaaggaaa agtcctcctg gatgaagccc cgatattttt aagcgcagaa
      181 gaggaagaaa agaagatcat agcccaggcg agcgcgccac tgcagaacga cggaatgttt
      241 aaaaatgata aggtaaaagt ccgttaccag gccgattacc cgatcattga acggcaagac
      301 gttgacctga tggacaccgc cccgaaccag atcgcttcga tagcggcttc tctcattcct
      361 ttcctcgagc acgacgatgc taaccgggcg ctgatgggct ccaacatgat gcgtcaggca
      421 gttcctctga tggagcctga agtgccactt gtcggaaccg gtatcgaagg ccaggttgtc
      481 agggattcca gggtacttat aaatgcagag ggcgatggtt atgttgaata cgtagatgca
      541 aaggaaattg ttatccggta tacccgggat gaaatggaaa aactggtcag ttttgatgaa
      601 gacataaaaa agtatgaact gaccaaattc ctgcgcacga accagaacac ctgcatcaat
      661 ttgcggccta tcgtcagaaa aggagaaaag gtcaccaaag ggcaggtatt gtgcgaaggg
      721 tatgcgacga aaaatggcga gctggcccta ggaagaaacc tgatggtagc atttatgccc
      781 tggaaaggtt acaattttga ggatgcagtc gtcatcaatg aaaaagtagt aaaagaagac
      841 atcttcacat ctatccacat cgaagaattc acgctggaag ttcgggacac caagagaggt
      901 attgaagaac tgaccaacga cattccgaac gtgagcgcgg aagcgaccaa agatctggat
      961 gaaaacgggc ttatccgcat cggcgctgag gtaaaagaag gggatattct catcgggaag
     1021 atcactccca agggagaaag cgatcctacg ccggaagaga aactactgag ggctattttc
     1081 ggtgataaag caggtgacgt taaaaacgca tcgctcaagg cgccaccagc tatgaaaggc
     1141 gttgtcatcg aaaataaact gttctcaagg atcattaaag accggaagtc aagatcaaag
     1201 gaaaaagatg tcctgaagga cctggacgac cagatgcaca aggaggtcaa tgacctgaaa
     1261 tccaagcttg tcgacaaact catgatcctt gtcagtggta aaacatcaca gggtgtttac
     1321 aataatttca aggaagaact ggtccccaag aaagccaagt ttacgcagaa aatgctgatc
     1381 gggctggatt atgccagtat caatccgaat aaatggacat ccgacaagga caaaaacgac
     1441 ctcattaaag tcctgatcaa caactataat atcaagcata aggaattgct tggccggttc
     1501 aaccggatga aattccagtc gaccgttggc gacgatcttc cgaacggtat cgtacaaatg
     1561 gccaaggttt atgttgcccg taagcgcaaa ttgcaggttg gcgataaaat gtcggggcgc
     1621 catggtaaca aaggtattgt ggccaggatc gtacgccagg aggacatgcc tttcctcgaa
     1681 gacggcaccc cagtagatat cgtactgaat ccgctgggcg tacccagccg tatgaacctc
     1741 gggcagattt atgaaactgt tctcggatgg gcaggataca aacttggcga aacctatggt
     1801 actccaatct ttgatggggc cagctaccag cagatcaatg aaaaaatgga agctgccggt
     1861 ttaccaaaag acggcaaggt ttacctttac gacggggaga ccggggagcg gttccatcag
     1921 cctaccactg ttggttttat ctatatgctg aagttacatc acatggtgga agataaaatc
     1981 cacgcccgtt ccataggacc atattcgctc attacacaac agccccttgg cgggaaagcc
     2041 cagttcgggg gacagcgttt cggtgaaatg gaagtttggg cgctcgaagc gttcggcgca
     2101 tccaatattc tgcaggagat ccttaccgtt aaatcggatg atgtccaggg ccgtgccaaa
     2161 acctacgaaa cgatcgtcaa aggcgaaaat atgcctaaac cgagcattcc ggaatcgttc
     2221 aatgtactcg ttcacgaact ccgtggcctg ggacttgagg taagttttga ataattaatc
     2281 agttaacatt aaaatcatta tacatggctt tcagaagaga aacgaatacc gttatcgact
     2341 tcaacagcat aacaatcagc ctggcctctc ctgaatcggt cctcgaaaga tcattcggag
     2401 aagtactgaa gcctgagaca atcaattacc ggacctataa acccgaacgc gacggtttat
     2461 tttgcgaaag gatattcgga ccggtaaaag attacgagtg ccattgcggg aaatacaaaa
     2521 ggatccgata caaaggaatc atttgcgacc gttgtggagt cgaggtaacg gagaaaaaag
     2581 tcaggcgtga gcggacaggt cacatccaac tggttgtgcc agtggcccat atctggttct
     2641 ttcggtccct tcccaacaag atcggcgctt tgctcggtct tccatccaag aaaatggatt
     2701 cgatcatcta ttatgagaaa tacgttgtta ttcagccggg tataaaagaa aaagacgggg
     2761 tcaaagagct agactttctc tcagaggaag agtattttga gatcgtcgac aacctgccgg
     2821 cagacaatgc tcacctggat gattccgatc cgaacaagtt cattgccaaa atgggagctg
     2881 aagctttatt tgaattgctg gaaaagcttg atctggataa agtttcatac gatctccgcc
     2941 ataaagccaa cacggagact tcgcagcagc gtaaacagga ggccctgaaa aggctgcagg
     3001 tttttgaagc tttccgtgat gcccggaccc gcaccgagaa taaaccgggc tggatgatat
     3061 tgaaaatcat tccagtgatc ccaccggagc tcaggccgct ggtaccgctt gatggcggac
     3121 gttttgcgac atccgacctg aacgacctgt accgcagggt gatcatccgc aataaccggc
     3181 ttaagaggct tattgagatc aaggcgcctg acgtgatcat gcgtaatgaa aaacgtatgc
     3241 tccaggaagc cgtcgactca cttcttgata attcccggaa ggtcagcgca gtcaagacag
     3301 aatcgaaccg tccacttaaa tcattgagcg acagtttgaa aggcaaacaa ggacgattca
     3361 ggcagaatct tctgggtaaa cgtgtggact attcgggccg gtcggtgatc gtggtgggtc
     3421 ctgagttaaa actcaatgaa tgcgggttgc cgaaagatat ggctgcagaa ttgttcaaac
     3481 cgttcattat caggaaactt cttgaaagag gcatagtaaa gacagtaaaa tccgctaaaa
     3541 aaatagtaga ccgtaaggat gctgttgtat gggatatcct ggagaatatt ctgaagggac
     3601 acccggttat gctaaaccgt gcccctaccc ttcaccggct tggtattcaa gcttttcagc
     3661 ctaaattgat cgaaggaaaa gccattcagc ttcatcctct ggtttgtacg gctttcaatg
     3721 ccgacttcga cggtgaccag atggcggtcc atgtaccatt gggcaatgca gcaattcttg
     3781 aggcacagtt acttatgctg gcatcgcaca acattctcaa tccagccaat ggcgccccta
     3841 tcactgttcc ttcgcaggac atggttttgg gcctgtatta tatgaccaag ggcaggaaaa
     3901 gcactcccga acacaaggta aaaggagaag gaatgcgctt ttattctcct gaagaggtta
     3961 tcatcgcatt taatgaagga aagcttgatt tgcatgccaa tatttcggtg aaagtggaag
     4021 acctggttga cggagttccc gaaaaccagg ttgttgaaac aactaccgga agagtcctgt
     4081 tcaatcaggt agttcccagt gagatgggat atatcaattc gctactgacc aaaaaagcac
     4141 ttcgcgatat tatcggtgat attctgaaag ctaccggtac caagaaaact gccgatttcc
     4201 tcgacaacat caaaaacctg ggatttatga tggctttccg cggaggattg tcgttcaacc
     4261 tgggagatgt cattgttccg aaggtgaaag aatcgttcat tgaagaagcc aaccaggagg
     4321 tggaagaggt gctgagtaat tacaatatgg gtttcataac caataatgaa cggtacaacc
     4381 agatcatcga tatctggacc cacaccaatt ccaagcttac gcaacaggtc atgaaagaga
     4441 tctccacgga tcagggtgga ttcaacccga tcttcatgat gctggattcc ggagcaaggg
     4501 gatccaagga acagatccgc cagttgtcgg gtatgagggg acttatggca aaaccgcaga
     4561 aatcgggagc taccggtggt gaaatcattg aaaacccgat cctctccaac tttaaagagg
     4621 gtttgtcggt tctggagtac ttcatttcaa cccacggcgc ccgtaaaggt ctggccgata
     4681 ccgctcttaa gactgcagat gcgggatatt taacccgtcg tctggtcgat gtggcccagg
     4741 atgtgatcat ttcggaggac gactgcggca ctcttagagg tataaccatt gctgctctca
     4801 agaaaaatga agaaatcgtg gaatcactct acgacaggat cctgggacgt gttacccttc
     4861 atgacatctt ccatccgaat tcaggtgaat tgattgccta tgccggacag gaactgactg
     4921 aagaaaagtg caagatgatt gaagattcgc cgatagaagc cgttgaaatc cgttcggtac
     4981 tgacttgtga gtcaaaacaa ggggtttgtg cacggtgtta cggacgaaac ctggcgactg
     5041 gccggatggt tcaggaagga gaggcggttg gcgtcatcgc agcccaatcc atcggggaac
     5101 cgggtaccca gctaacactt cgtactttcc acgttggggg tactgcatcg aacgtagcta
     5161 ttatttcaaa gattgaagcc aaatacgacg gtatccttga aatcgatgag ctccggttcg
     5221 tccctttcac caaccgtgag ggcaaatcat tcgatgttgt gatcggcagg tcggctgaaa
     5281 tgaggattgt cgacaaacat acccgtatcg tattggcatc agggcatatt ccgtatggcg
     5341 ccaacttata tttcaaagag aatacggaag tcagaaaagg caacctgatc agcgactggg
     5401 atccattcaa cgcagtaatt ctcgccgaag ttaagggttc gatccagtat gaaaatatcg
     5461 ttgaaggaaa gacttaccgt atcgaatcgg acgaacagac cggttatgtc gataaggttg
     5521 tcattgaacc ccggcaaaag ttgaagaatc ctaccattaa tatcatatcc ggcgacggag
     5581 aaatattaaa ggcttatgat atcccggtcg gcgcacgtat tatagtggaa gacgggcagg
     5641 aaatcaaatc gggcgagccg ctggtgaaga ttccgagagc catcggcaag acaggagaca
     5701 tcacgggagg tcttccgcgc gtaacagagc ttttcgaggc caggaatcct tccgaaccgg
     5761 ctgttgtagc cgagattgat ggtgttgtta cgatcaacaa gaagctgaaa aggggtaacc
     5821 gtgaagttat catcacctct aaaacagggg aagaaaagca ttacctgatc ccaacgacaa
     5881 aacagatcct ggcacaggaa aatgactata tccgcgccgg tacgcctctg tcagaaggag
     5941 aaatttctcc ttccgatatc ctcgctatta aaggccctat gaaggtccag gaatatattg
     6001 taaatgaggt acaggaagtt taccgtctcc agggtgtgaa gatcaatgat aaacactttg
     6061 aggtcattgt ccgtcagatg atgcgcaaag tagaagtgga tgatcccggt gatacccggt
     6121 tccttgaagg ggaactggtt aacaaaaccg atttccagga tgaaaacgat gatgttttcg
     6181 gaaaaaaggt tgtggttgat tccggtgatt cgctctctta cagggccggc cagattgtca
     6241 gcgcccgtaa attaagggat gagaattcca tgctgaaa
//
LOCUS       DAOPNH010000203         6263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14340_length_6263_cov_5.949742, whole genome shotgun sequence.
ACCESSION   DAOPNH010000203 DAOPNH010000000
VERSION     DAOPNH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14340_length_6263_cov_5.949742"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2446
                     /locus_tag="PKZ74_10520"
     CDS             <1..2446
                     /locus_tag="PKZ74_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM93234.1"
                     /translation="ILLVEDSIRFYSSYLPNIYKIIFKQSKDFMTEGLNEHQMMLRMR
                     GRPKIMLATTYEEALGLYEKYKRNLLGIITDMSYPRNGKQEKYAGVKFCEKVQRDDPF
                     MPILLQSSDKEGDEVARRLNVGFINKSSKTISVELKNYIREYFAFGDFVFIDPDTGEE
                     IARAVDLKGLQELLFQVPDEALRYHLSRNQLSKWLRARALFPLAEMLVDITLDDFTDL
                     DEVRRYLFDAIASFRISKSRGVIAQFRRDRFDEYLTFTRIGEGSIGGKARGLAFLDML
                     IKRNRILEKWPGVYITIPRTVVLCTDIFDEFMEENNLYEVALSDRSDEEILKEFIHAR
                     LPFRIHEDLYTLISYVRSPIAVRSSSLLEDSHYQPFAGIYSTYMVPYVMDNERVMMEM
                     LSNAIKSVYASTYFKASKAYMTATSNVIDEEKMGIVMQTVAGTKYGNRFYPSFSGVAR
                     SINFYPLEPEKPEDGVASIALGLGKYIVDGGLALHFSPKYPKRVLQTYSPEAALRETQ
                     KYFYALDMHPEMFKPNIDDGINILKLPIKEAEADGSLKWVASTYDLQNGTLRDGVNYE
                     GKKLVTFSNILKHNVFPLPEILQTILEIGQKEMGKPVEIEFAVDLNRPASDPKVFYCL
                     QIRPIVDNKESISTDLEKVPMDKTILYSKSALGNGSINDLTDIVYIKPESFNPARNTD
                     LVNRISKINDQFRTEQKNYILIGPGRWGSQDPWLGIPVKWPQISAARVIIESGLENYR
                     IDPSQGTHFFQNLTSFRVGYFTINPYLNDGYYDLAYLNAQPAIQEDEYIRHIRFESPL
                     LVEIDGKKNLGVIYKP"
     gene            complement(2453..2653)
                     /locus_tag="PKZ74_10525"
     CDS             complement(2453..2653)
                     /locus_tag="PKZ74_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPM93235.1"
                     /translation="MKKNMGSADKVIRLLLAAVIILLFILNVVSGILGYVLLAFAFIF
                     IVTSMVSFCPLYTIFGIKTCKK"
     gene            2860..3351
                     /locus_tag="PKZ74_10530"
     CDS             2860..3351
                     /locus_tag="PKZ74_10530"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93236.1"
                     /translation="MTSCGSRSSDQEILATKIQYDVPVVNNDPQLDWWINNLEGSKRE
                     PFLKRMMEAAEKGEVRVFDYFNNPLTPGEVISAGRDTVYATLLRNDPPYEEYDTAIIS
                     TVTYKDISRIRFLEEWRYDPATLQIDKKVVAIGPVMIKESGGQTYNQLLFWIYTDDSY
                     KPE"
     gene            3388..4623
                     /locus_tag="PKZ74_10535"
     CDS             3388..4623
                     /locus_tag="PKZ74_10535"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM93237.1"
                     /translation="MEFQTVTFPNNIRFLHIPAAGPVAHIAVYINAGTRDEDDRQSGM
                     AHFIEHTIFKGTERRKAYHVLVRLESVGGELNAYTAKEETCIFATFLKEHFTRALDLI
                     SDIIIHPTFPGKELQKEKDVILDEINSYKDNPAEEIYDVLEENVFKNHALGRNILGKP
                     DDIGRFTREDALGFIRSQYTGGRMVVSTIGDLDYRKIGQQVRKYFEAVPAEGQNGERK
                     AFSGYNPFSLSMTRTTYQAHCMMGNIAYDRKDPRRYPLFLLNNILGGPAMNSRLNLAV
                     RERHGYAYTIESAYLPYTDTGIFSLYFGTDNDNLDKSLRLIRREMDKLRTEKLGGLQL
                     SQARKQLIGQIEIANESNLAFLMAAGKAFLHDTRLDDSEEIRKQIEAITASQIMEVAQ
                     EILDPERMSLLIFKSARND"
     gene            4616..5254
                     /locus_tag="PKZ74_10540"
     CDS             4616..5254
                     /locus_tag="PKZ74_10540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPM93238.1"
                     /translation="MIDPRIEDYIEQHTSPEPELLYELRRQTYLRTPYPRMLSGPVQG
                     RFLEMISRMIKPVTILEIGTFTGYSAICLAQGLAGGGLLHTIEANPVFADLAESYFEK
                     AGLSNRIVLHRGEALSVLPNLDGLFDLVFIDAAKEQYLDYYQMVFDKVKTGGFILADN
                     TLWDGRVLENEDVTDPETAGIIRFNRFVNEDERVTNVLLSVRDGILIIQKIV"
     gene            5434..5916
                     /locus_tag="PKZ74_10545"
     CDS             5434..5916
                     /locus_tag="PKZ74_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93239.1"
                     /translation="MEATNQTFECCPPFDPAPWTDTRITWEGKRFIRDKVVTVFYMPL
                     NFGSVMKRLDKAVRGAGATMPDYLCLSDHTSSWNMDLYLAVDKEVPGAENMTLSGKYY
                     SKVYEGPFKDTGKWMKDAAADVKSRGMAVKKWYMWYTTCPKCAKKYGKNYVVVIGEVE
                     "
     gene            6033..>6263
                     /locus_tag="PKZ74_10550"
     CDS             6033..>6263
                     /locus_tag="PKZ74_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93240.1"
                     /translation="MHKSNILTGAIFLFCLSQIPFGTFSQAVQVEGNAFLEDQTYHNG
                     IMIAFERFAPQYLYDTAYSDSAGYYSIQLEQGM"
BASE COUNT         1701 a         1470 c         1604 g         1488 t
ORIGIN      
        1 tattttgctc gtggaagact ccatccggtt ttattccagt tatcttccca acatctacaa
       61 gatcatcttc aaacaatcga aagacttcat gacggaaggg ctgaacgaac accagatgat
      121 gctgcggatg cggggacgcc ccaagatcat gctggcgacc acgtatgagg aggcgctggg
      181 tctctatgag aaatacaagc gcaacctgct ggggatcatt accgatatga gctatccgcg
      241 caacggcaaa caggagaagt acgccggtgt gaaattctgc gaaaaagtgc agcgcgacga
      301 tccgtttatg cccatcctgc tgcagtcgtc ggataaagaa ggggatgaag tggccaggag
      361 gctgaatgtc gggttcatca acaagagttc taaaacgata tcggtagagc taaagaatta
      421 tatccgggag tattttgcct tcggcgattt tgtttttatt gacccggata ccggtgaaga
      481 aatagccagg gcggtggatt tgaaaggtct gcaggaattg cttttccagg tgccggacga
      541 agcgctgcgc tatcatctca gccgcaacca gctctccaaa tggctcagag ccagggcgtt
      601 attcccactc gctgagatgc tggtcgatat taccctcgac gattttaccg acctggacga
      661 ggtgaggcgc taccttttcg atgccatcgc cagcttccgg ataagcaaat cgcggggggt
      721 catcgcccag ttccggcgcg accggttcga tgaatatctt acttttaccc gcatcgggga
      781 aggatcgatc ggcggaaagg cccgcggact ggcatttctc gatatgctga tcaaacgcaa
      841 ccgtatcctg gaaaaatggc cgggagtgta tatcaccatt cccaggaccg ttgttctttg
      901 taccgatatc tttgatgagt tcatggagga aaacaatctc tatgaagtgg ctttgtcgga
      961 tcggagcgac gaggaaatcc tgaaggaatt catccatgcc cgtctgccgt tccgtatcca
     1021 cgaagacctg tacacgctca tctcctatgt caggagcccc atcgccgtaa ggtcgtcaag
     1081 cttactggaa gattcacatt atcaaccgtt tgccgggata tattcgacct atatggtgcc
     1141 ttatgttatg gataatgaaa gggtgatgat ggaaatgctc agcaatgcga tcaaaagcgt
     1201 atatgcatcg acttacttca aagccagcaa agcttacatg acggcgacat ccaacgtgat
     1261 cgacgaggag aagatgggga ttgtgatgca gacggtggca ggcaccaaat acggtaaccg
     1321 cttctatccc tctttttcag gcgtggcccg gtccatcaac ttttacccgc tggaacccga
     1381 aaaaccggag gatggagtgg ccagcattgc gctcgggctc gggaagtata ttgtcgacgg
     1441 cggccttgcc cttcatttct cgccaaaata tccgaagcgt gtacttcaga cctattctcc
     1501 ggaagccgcc ctcagggaga cccagaagta tttctacgcc ctggatatgc acccggagat
     1561 gttcaaaccc aatatcgacg atgggatcaa tatcctgaaa ctgccgatca aagaagccga
     1621 agccgacgga tcgctgaaat gggttgcttc cacgtacgac ctacagaacg gcacacttcg
     1681 cgacggagtc aattacgaag ggaaaaagct ggtcaccttt tcgaatattc tgaagcataa
     1741 tgttttcccg ttgcctgaga tcctgcagac catcctggaa atcggccaaa aagagatggg
     1801 gaaaccggtg gagatcgaat ttgccgtcga cctcaaccgg cccgcttccg atccgaaggt
     1861 tttctattgt ctgcagatca ggcctattgt cgacaacaaa gagagcatct cgaccgacct
     1921 ggagaaggta ccgatggata agaccattct ctattcaaaa tcggctcttg gaaacggctc
     1981 catcaacgat ctgaccgaca tcgtctacat caaacccgag tcgttcaacc cggcccgcaa
     2041 tacggatctg gtgaacagga tcagcaagat caacgatcag ttcaggacgg agcagaagaa
     2101 ctacattctg atcggaccgg gccgctgggg ctcgcaggat ccatggctcg gaatcccggt
     2161 caaatggccc cagatctcgg ctgcaagggt aataatcgag tcagggctcg agaattaccg
     2221 gatcgatccc agccagggaa cccacttttt ccagaacctg acctccttcc gggtcggata
     2281 ttttacgatc aatccgtacc ttaacgacgg ctattatgac ctggcctacc tgaatgccca
     2341 accggccatc caggaagatg aatatatccg ccacatccgg tttgaaagcc cgttgctggt
     2401 ggagatcgac ggaaagaaga acctcggcgt gatttataag ccctgagcag aactactttt
     2461 tacatgtctt aataccgaat atggtataga gcgggcagaa gctgaccatg ctggtcacaa
     2521 taaagataaa ggcaaaggca agaaggacat accccaggat accggaaacg acatttaaaa
     2581 taaagaggag gatgattaca gcggctaata ataaccggat gactttgtcg gcagagccca
     2641 tgttcttttt cataatgttg atttttaaga tggtggttta aaaaatttgt ttgatgtaat
     2701 aacaactaaa aatataaaaa gtttatgccg gaataatgat taagaaggat atttgcaatt
     2761 aatttgtgat agagttaccg ccaatcaaat caatatgtta tgaatacccg gaatcgcatt
     2821 ttagccttgt tattgtcaac gggcgtaatt ttttcaatga tgacatcctg tggaagccgt
     2881 tcttcagacc aggaaatcct ggcgacgaag atccagtatg atgtgccggt cgtcaataac
     2941 gatccgcagc tcgactggtg gatcaacaac ctggagggat cgaaacggga gccatttctg
     3001 aagcgaatga tggaggctgc cgaaaaaggg gaggtgcggg tttttgacta tttcaacaat
     3061 ccactcaccc ccggagaggt tatttcggcg ggcagagaca cggtttacgc aacattgctg
     3121 aggaacgatc ctccgtatga agagtacgac acggctatca tctcaaccgt aacctacaag
     3181 gatatttccc ggatcaggtt cctggaggag tggcgctacg atccggccac cctgcagatc
     3241 gataaaaaag tcgtggccat cggtccggtc atgattaaag agtcgggagg gcagacgtac
     3301 aaccagcttc tgttctggat ttatacagac gattcttaca aacccgagta acccattatt
     3361 cctatatttg cggttcatca acagaagatg gaatttcaga cagttacttt tccgaacaat
     3421 atccgtttcc tccatattcc tgcagccggg cccgtggccc atatcgctgt ttatatcaat
     3481 gccgggacaa gggatgagga tgacaggcag agtggaatgg cccactttat cgagcacacg
     3541 atattcaaag gaaccgaaag gcggaaagcc taccacgtac tggtccgact cgaaagcgtc
     3601 ggaggagagc tgaatgccta tacggccaaa gaagagacct gtatttttgc taccttcctg
     3661 aaggagcatt tcacccgggc attggatctt atctcggata ttatcattca tccgactttt
     3721 cccggaaaag agctgcagaa agagaaagat gtcatcctgg atgaaatcaa ttcctataaa
     3781 gacaatcccg ccgaggagat ttacgatgtg ttggaagaaa acgtttttaa gaaccatgcc
     3841 cttggcagga atattcttgg aaaacccgat gatataggcc gttttacacg ggaagatgcc
     3901 ctgggtttta tccggagtca gtataccggc gggagaatgg tcgtcagtac gatcggcgac
     3961 cttgactacc ggaagatcgg ccaacaggtc aggaaatatt ttgaagcggt gccggcagaa
     4021 ggccagaatg gcgaaagaaa ggcattttca ggttataatc cgttcagcct gagcatgaca
     4081 cgtacgactt accaggccca ttgcatgatg gggaatattg cctatgaccg gaaagatcca
     4141 cggcggtacc cgctgtttct gctgaacaac atcctgggcg ggccagcgat gaattccagg
     4201 ctcaacctgg ccgtcaggga gcgtcacgga tacgcataca cgatcgaatc ggcatacctg
     4261 ccttacaccg atacagggat cttcagcctc tatttcggaa cggataacga caacctggac
     4321 aaatcgctcc ggctgatccg tcgtgaaatg gacaagctgc gcaccgaaaa gctgggcggg
     4381 ttgcaactga gccaggcgcg gaaacagctt atcgggcaga tcgaaatcgc caacgaatcc
     4441 aatttagcct tcctgatggc cgccggcaag gctttcctgc acgatacccg tctcgacgat
     4501 tctgaagaga tcaggaagca gatcgaggcc atcactgcca gccagatcat ggaggtagca
     4561 caggagattc tggatccgga gcggatgagc ctgctgatct ttaaaagcgc ccgcaatgat
     4621 tgatccccgg atagaagatt acatcgaaca gcacacctcc ccggagcccg aattgctgta
     4681 tgaactcagg cgacaaacct atctccgcac cccatatccc cggatgcttt caggcccggt
     4741 gcaggggcgg ttcctggaga tgatcagccg gatgatcaaa ccggtgacca tactcgaaat
     4801 cgggaccttt acaggctatt cagcaatttg ccttgcccag gggctggccg gcggcgggct
     4861 actgcatacg atcgaagcca accctgtctt tgctgacctg gctgaaagct attttgaaaa
     4921 ggccggactg agtaaccgga tcgtccttca ccggggggag gctttatctg ttcttccaaa
     4981 tctggatggc cttttcgacc ttgtttttat cgatgctgcc aaagagcaat acctggatta
     5041 ttaccaaatg gtttttgata aagtgaagac gggaggattt atcctggccg acaatacttt
     5101 atgggatggg agggtgctgg agaacgagga tgtcacggat cccgaaacgg ccggtatcat
     5161 ccggttcaac cggtttgtga atgaagacga gcgagtgacg aatgtgctgc tttcggtcag
     5221 ggatggcatc ctgattattc aaaaaatcgt ttgataatac atttaattgg cgtagatttg
     5281 aagaatgatt tggatttatc attcttccta ctctgtgtaa ctctcacgcc ttgcgggaca
     5341 agctgcgtta atctgtgtac tctgtggtga aaaaggaata aagactaaaa tatttttatc
     5401 tgattaatca tattaatcta aaaatcaagt atcatggaag caaccaacca aacctttgaa
     5461 tgctgtcctc cctttgatcc ggcaccatgg accgacacgc ggataacctg ggaagggaaa
     5521 agatttatca gggacaaggt cgtgactgtt ttctacatgc ccctgaattt cggcagtgtc
     5581 atgaaaagac tggataaagc tgtgaggggt gccggagcga cgatgcccga ttacctctgt
     5641 ttgtcggacc acacctcctc ctggaatatg gatctctacc tggcggtgga caaggaggtg
     5701 cccggtgctg aaaatatgac cctgagcgga aagtattaca gcaaggtcta cgaaggtcct
     5761 tttaaagata ccggtaaatg gatgaaagac gcggcagccg atgtgaaatc cagaggcatg
     5821 gcagtgaaga aatggtacat gtggtacact acctgcccga aatgtgcgaa gaagtacggg
     5881 aagaattatg tggtagtgat aggggaggtg gagtagggca taggactata ggacggtaag
     5941 agaataagaa ggcagtagat cccagtgctt gggattgaat ggtttaggtg cgataatcgt
     6001 gtaattttct gataattttt aatcaaacca ttatgcataa atcgaatatt ttgacaggag
     6061 ccatattttt attctgtttg tcacagattc ctttcggcac tttttctcaa gctgtccaag
     6121 ttgagggtaa tgctttcctc gaggatcaaa cttaccataa tggcataatg atcgcctttg
     6181 agcggtttgc gccccaatat ctttatgata ctgcctattc ggactctgcc ggatattatt
     6241 ctattcaact cgaacagggt atg
//
LOCUS       DAOPNH010000204         6254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14372_length_6254_cov_6.043555, whole genome shotgun sequence.
ACCESSION   DAOPNH010000204 DAOPNH010000000
VERSION     DAOPNH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14372_length_6254_cov_6.043555"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKZ74_10555"
     CDS             <1..256
                     /locus_tag="PKZ74_10555"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HPM93241.1"
                     /translation="LRETLFIRIHYPVQVFAVYHDENLNGVMDANEKGFPKEGYGFST
                     KNKMLGRAKFEQAAFEVSGDQAVEVKMIYPAGSTVNSNKN"
     gene            338..718
                     /locus_tag="PKZ74_10560"
     CDS             338..718
                     /locus_tag="PKZ74_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPM93242.1"
                     /translation="MTENEIAKIVFEKGLKVHKALGPGLLESSYEECLYYELCKSGLQ
                     VEKQKALPLVYEDVKLEVGYRTDLIIDNKRIVELKAVDALNEIHLAQILTYLRLSGCK
                     LGLLINFNTLMFKDGVRRVINGTL"
     gene            840..1475
                     /locus_tag="PKZ74_10565"
     CDS             840..1475
                     /locus_tag="PKZ74_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM93243.1"
                     /translation="MKPIKIYILIVLLPIISFSQPAVDVPVKDPGNLIAQIAYFSRNT
                     TSITAGFIQVKEMSFMEETVTSSGRFYFQKDDKVRWEYTEPFSYVIIVSGDRIRIIDE
                     GKQKDFDAGSGRMFREVSRVMTGMVDGTLLTDGRFAVSWLEAPDYYRAELVPVSGAMK
                     DYLSGIGLKLNKTDHSVDELKMVEKSGDYTIITFNNKKFNATIPPEVFGLD"
     gene            1438..1830
                     /locus_tag="PKZ74_10570"
     CDS             1438..1830
                     /locus_tag="PKZ74_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93244.1"
                     /translation="MQRSLLRYSAWISLVFLAVSCFSPRITVTGEQPASYDKTLQPGL
                     PEKIIYKATVTLRGQELTGRLMLKKTDDDRYRVAWYSEMGMTYLEGTMDISSEKKTFI
                     IQNIIPALDKKMFLRKFEKSVRQVYNDS"
     gene            complement(1880..3409)
                     /locus_tag="PKZ74_10575"
     CDS             complement(1880..3409)
                     /locus_tag="PKZ74_10575"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93245.1"
                     /translation="MKIVTFFLILLFGHLILNAQFIYFNNRYNNDNWSASLSIIETDY
                     GYVIAGVSGVASGEYIFKRIVLTAIDDEANQLWWKTYGEDFHNYYAGFMRGCIKTSDG
                     GFAISGGIQDSIRHVGTLVKFDEDGDLSWIRKYGDTVSTYYTSTIFTVCNQLPDNGYL
                     ITGTFWVSEDDQDIILIRTDSLGNIKWSHTYGQLHWSDGGYSIAQLPDGYFLIGLDKE
                     KVGNNTLTDGGLLKVDSLGNQIWIKYYGGIYDDGYSQVVLSQDGNYLIGSTYAVSQPH
                     PDYPDHKIWLKKTDTSGNIIWERKYSDTVFMGWCSAVEELENGDIIVSGTGAFADGFG
                     NYGWILKTDENGDSLWMRRYSYYPTFENHLNDMRITSDSGLIITGMAFGEPDWEQSIW
                     VQKLDSIGCDSSGCDTTVGIVQLFPPFSGSAGISIYPNPASDRINFVLRQISLSTFYA
                     SNRELEIFNLFGEKMAGLKISVFQETYSFDVSSFPPGFYIAVIREKQKVTWCGRFMVD
                     R"
     gene            complement(3500..6031)
                     /locus_tag="PKZ74_10580"
     CDS             complement(3500..6031)
                     /locus_tag="PKZ74_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93246.1"
                     /translation="MPEDASIIVKRGGQLTINGGLVTSYDTVLWQGIQVWGDASASQS
                     TAGAQGKITIVNGGTIENAIIAVRLAKIDGLKYEAGFEGGIIQTNDAHFINNRTGVKF
                     YPYRNMVSGVEMDNLSYFRNSEFLTGAELADESTPEDFLHLNGVKGINISGCSFTNSR
                     PEQEAGMSDRGIGINSYDASFTVSWYCASSYPCEEPVLSTFTNLNYGINARNATTSGA
                     FDVNHSEFNNNLTGIYFNNIDYADIIFNDFELYNVSLFHEPDDIFGGLYIDNCSGYTI
                     EENNFFNDDEYHPQNEIRSIGITVNNSGSDLNMIYRNTFNRLHLGILAQNGNRGTSIT
                     TGLKLKCNICTNNEDDFAVTADTSSANMGISIYQGAFILNEISAPAGNLFSHNENSDP
                     YCDFNNTLSSQFIYYIHHDGTNEDPWVPVYYQNIIPCPYIEYEDYEKACPTNFSIGGS
                     NLKSEPNVMNEEELRSRMILMENLRDSAANDLSLWIDAGNTPELNYEVESSTPDESLE
                     LYDELIADSPYLSGTVLSSSIEKEEVLVNAMIRDIMVANPHGVKKEELIGALEQRIPP
                     VPDYMMAEIMAGLDSIAQKELRESEIAWYAQERELAFDKLMKIYLCDSNQESAGDSLV
                     ALILDHGNINSHYYLATRYLEQNNFTNATLILVDIPEQFELEPGQQAEYQDYQTVFSI
                     ITNVLQEGMPLDSIDNTSRQILYQIAENDSRPAIIAQNILKQIDSVDYPEIYILPTSG
                     PITRRYLAEIPDKDSDPEQGNLFRVYPNPGRDYFIIEYSFERVPDQASYSISDQYGRI
                     TEQCPIEGKQNQLIRRTTSYSSGLYTIKLIINGNTEKTLKINIIK"
BASE COUNT         1791 a         1336 c         1289 g         1838 t
ORIGIN      
        1 ccttcgggaa acccttttca ttcgcatcca ttaccccgtt caggtttttg ccgtgtacca
       61 cgacgaaaac ctgaacgggg taatggatgc gaatgaaaag ggtttcccga aggaggggta
      121 tggtttttcg acgaagaaca agatgctggg ccgggcgaag ttcgagcagg cggcatttga
      181 ggtttcagga gaccaggctg tggaggtaaa gatgatttat ccggctggtt caactgtcaa
      241 ttctaataaa aattaatttt atttaaccac aaaggacaca aggggttaca ctaagagcac
      301 aaggatttta atacaaattt taaagaaggg aggatatatg acggagaatg aaattgctaa
      361 aatcgtgttc gaaaaggggc ttaaagtaca taaagcctta ggtcccgggc tactggaaag
      421 ttcgtatgaa gaatgtcttt attacgaact ttgcaaatca ggattgcaag tagaaaaaca
      481 aaaggctttg cctctggttt atgaagatgt aaaactggaa gtaggatata gaacagattt
      541 gataattgat aataagcgaa ttgtggaatt aaaagctgtt gatgcattga atgaaattca
      601 tctggctcag atcctgactt acctcaggct ttcaggatgt aaactgggat tattgatcaa
      661 ttttaataca ctgatgttta aagacggtgt tagacgtgtt atcaatggaa ccttgtgatc
      721 tttgtgtaat cccttgtgtc ctttgcggtt aaaaaataaa gatggataaa aagactaatc
      781 aacagaacct tgtgatcttt gtgaaaccct ttgtgtcctt tgcgtttaag taaatcacga
      841 tgaaaccgat aaaaatctat atcttaatag ttcttcttcc gataatttcc ttttcccaac
      901 cggcagttga tgttcctgtt aaagatcccg gtaatcttat cgctcagatc gcctatttct
      961 cccggaacac cacctccatc accgccggtt tcatccaggt aaaagaaatg agtttcatgg
     1021 aagaaacggt cacctcttcg ggccggtttt attttcagaa agacgacaag gtcaggtggg
     1081 aatacaccga gcctttcagc tacgtgatca ttgtcagcgg cgatcggata cgcatcatcg
     1141 acgaagggaa gcagaaggat ttcgatgcgg gatcgggacg gatgttcagg gaggtaagcc
     1201 gggtgatgac ggggatggtc gacgggacgc tgctgaccga cggccggttt gcagtgagct
     1261 ggctcgaagc gccggattac taccgggcag agctcgtccc ggtcagcggc gccatgaagg
     1321 actacctgtc aggaatcgga ttgaagctga ataagaccga ccattcggtt gatgaactga
     1381 aaatggtgga aaagtcgggc gattacacga tcattacttt taacaacaaa aagttcaatg
     1441 caacgatccc tcctgaggta ttcggcctgg attagcctgg tattcctggc cgtatcctgt
     1501 ttttctcccc ggatcaccgt tacgggagag cagccggctt cttacgacaa gaccctgcag
     1561 ccaggccttc cggagaagat catttacaag gctaccgtga cgttgcgggg ccaggagctg
     1621 accggcaggc tgatgctgaa aaagacggat gacgaccgat accgcgtagc gtggtacagt
     1681 gagatgggaa tgacctacct ggagggaacg atggacatct catccgaaaa gaaaacgttc
     1741 atcatccaga acattattcc tgcccttgat aagaagatgt ttttaaggaa gtttgaaaag
     1801 tcggtaagac aggtctataa cgattcttaa aatggattgg atgtccgccc acaaggctgc
     1861 aaagctccaa cgataatcct tacctatcaa ccatgaacct accgcaccag gtaacttttt
     1921 gcttctccct gatcacggct atgtaaaatc cgggcggaaa ggatgaaaca tcaaatgaat
     1981 aagtctcttg gaaaaccgat atctttaatc cggccatttt ttcgccgaaa aggttaaaaa
     2041 tttccagttc tctgttactt gcatagaagg tactcaggct aatttgccgg agtacgaaat
     2101 tgatccggtc ggaagcagga ttcggatata ttgagatgcc ggctgatccc gaaaatggcg
     2161 gaaacaattg aacgattccg acagtagtat cgcaaccgga agaatcgcac ccgatgctat
     2221 cgagtttctg aacccagatg gattgttccc agtccggctc tccaaatgcc attcccgtaa
     2281 taatgagccc actgtctgaa gtaatccgca tatcattcaa atggttttcg aaagtagggt
     2341 aataactata tcgtcgcatc caaaggctat cgccgttttc atctgttttt agtatccagc
     2401 cgtaattgcc gaatccatct gcaaatgcgc ccgtccctga aacaataata tcgccatttt
     2461 ccaattcttc tacagcagag caccagccca taaagaccgt atcactgtat ttccgctccc
     2521 aaataatatt tccggatgta tctgtttttt ttagccatat tttatgatcc gggtaatccg
     2581 gatgtggttg tgatacggca taagtacttc caatcagata attgccatct tgtgaaagaa
     2641 caacttgtga atatccgtca tcgtatatac caccatagta cttaatccaa atctgatttc
     2701 cgaggctatc tacttttaat aagcccccat cagttaaggt attattacca accttttcct
     2761 tatctaatcc gattaaaaaa taaccgtcag gtaattgagc gattgaataa ccaccatctg
     2821 accagtgtaa ttgaccatag gtatgtgacc atttaatatt tccgaggcta tctgtcctga
     2881 tcagtataat atcctgatca tcttctgaca cccaaaatgt acccgtaata agatagccgt
     2941 tatctggcaa ttgattgcaa accgtaaata ttgtagaggt ataataagtc gaaaccgtgt
     3001 cgccatattt tctgatccag cttaaatcac catcctcatc aaatttcacg agagtcccga
     3061 catgcctgat ggaatcttga attccaccac tgattgcgaa tccgccatcc gacgttttta
     3121 tgcaacccct cataaaaccg gcataatagt tatggaaatc ttccccatat gttttccacc
     3181 acagttggtt agcctcatcg tcgatggcag taagcacaat ccttttgaaa atatattcac
     3241 cagaagccac accactgact ccggcaatta cataaccgta atccgtttca atgatgctta
     3301 aacttgcgct ccaattgtcg ttgttatacc ggttgttaaa atatatgaat tgagcattta
     3361 agatcaaatg tccaaataat aatatcagga agaaggtaac tattttcatt gcagaaggtt
     3421 tatgctgcat tactacttaa aaagattccc tcaatatttc gctattgaag gaatcttagc
     3481 atttaagaat tctatgaatc tatttaatta tatttatttt cagggttttc tcagtatttc
     3541 cattaataat taacttgata gtatacaagc ccgagctata tgaagtcgtt ctcctgatca
     3601 attgattttg ttttccttca atcgggcatt gttctgtaat tctaccatac tggtcgctta
     3661 ttgaataaga tgcctgatcc ggaactcgct caaaggaata ttcaatgatg aaataatcgc
     3721 gtccgggatt cgggtatacc ctgaataggt tgccctgttc cggatcagag tctttatccg
     3781 gaatctcagc caaataccgt cgtgtaattg ggcccgatgt gggcagaata tatatctccg
     3841 gatagtccac gctatcaatt tgtttcagaa tattttgtgc aatgatagca ggtcttgaat
     3901 cattttccgc gatctggtat agaatctgac gcgaagtatt gtcaatcgaa tccaatggca
     3961 taccttcttg cagaacatta gttataatgg aaaacactgt ctggtaatcc tggtattcag
     4021 cttgttgccc gggttccagt tcaaattgtt ccgggatatc aacaaggatt aaggttgcat
     4081 ttgtgaaatt attttgctca aggtacctgg tcgccagata ataatggctg ttgatatttc
     4141 cgtgatccaa aataagtgcg accagactgt ctccggccga ttcctgattg gaatcgcaaa
     4201 gatagatctt cataagtttg tcaaaggcta attctctttc ctgggcatac caggcaattt
     4261 cgctttcccg taactctttc tgcgcgattg aatccaatcc ggccatgatc tctgccatca
     4321 tataatcggg cactggcggt attcgttgtt caagcgcccc gataagttct tcctttttaa
     4381 caccatgcgg attggcaacc ataatatcac ggatcatggc attgaccagc acctcctctt
     4441 tttcaatact tgatgagaga accgtgccgg aaagataagg cgaatcggct ataagttcat
     4501 catatagctc cagtgactca tccggggtag agctttctac ctcataattc agttcaggag
     4561 tattcccggc atcgatccat aaactcaagt cattcgctgc tgaatctctg agattttcca
     4621 tcaatatcat cctggatctc aattcttctt cattcatcac attcggttct gattttaagt
     4681 tactgcctcc tatgctaaaa tttgtggggc atgccttttc ataatcctca tattctatat
     4741 aaggacatgg gatgatattt tggtaataaa ccggaaccca cgggtcttcg tttgttccgt
     4801 catgatggat ataataaata aactgcgatg atagggtatt attaaaatca caatatggat
     4861 cactattttc gttgtgagag aataaattgc ctgctggtgc agatatttcg ttaagaataa
     4921 aagcaccttg gtagattgaa attcccatgt tggcagaaga agtgtcggct gtcactgcaa
     4981 agtcatcttc attattggta caaatattac atttcagctt taatccagta gtaattgaag
     5041 tccctcgatt gccattttgt gcaagtatac caaggtgtaa acgattaaat gtattccgat
     5101 agatcatgtt gagatcgctt ccggaattgt ttacggtaat cccaatactt ctgatttcat
     5161 tctgcggatg atattcatcg tcattaaaga aattattctc ttcaatggta tagccggaac
     5221 aattatcgat ataaagccct ccgaaaatat catccggttc atggaacagg ctcacgttat
     5281 acagctcaaa atcattaaaa attatgtctg cataatcgat attattaaaa taaattcctg
     5341 ttaaattgtt attaaattcc gaatggttca catcaaaggc tcctgaagta gtggcattcc
     5401 gggcatttat gccgtaattc aggttggtga acgtgcttaa caccggttct tcacagggat
     5461 atgacgatgc acaataccag gaaacggtga agcttgcgtc atagctgtta atgccaatgc
     5521 ctctgtcgct cattccggct tcctgttccg gccttgaatt ggtaaaagag cagccactaa
     5581 tatttatccc ctttacacca ttgagatgga ggaaatcttc gggagtactt tcgtcggcca
     5641 gttcggcgcc ggtaagaaat tccgaattcc ggaaatagct caggttgtcc atttccacac
     5701 ctgagaccat gttcctgtaa ggataaaact taacgcctgt ccggttgtta atgaaatggg
     5761 catcgttagt ctgaatgata ccgccttcaa atccggcctc atactttagc ccatcaatct
     5821 tagccagcct gactgctatt atggcatttt caatagtgcc gccgttcacg atcgtaattt
     5881 ttccctgggc gcccgcggtc gattgagagg ccgatgcatc gccccacacc tggatgcctt
     5941 gccataatac ggtatcataa cttgtgacta aacctccatt aattgttagt tgtccacctc
     6001 ttttaacaat gatactcgca tcttccggca tagataattt gcaagttaat tctaaaacac
     6061 ctccatcttc aattataata tcactataaa ctcttaagtt ataattccaa gaaacagtat
     6121 cgttaatatt atagcttgat tccggaaagt aagtacttgg atctatatat ctttttgtat
     6181 tagatgtatg taaatatcta atcatttgtc caatttcaga gggtggtaac caacaggcta
     6241 ttagagaaga ttga
//
LOCUS       DAOPNH010000205         6243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14401_length_6243_cov_5.008242, whole genome shotgun sequence.
ACCESSION   DAOPNH010000205 DAOPNH010000000
VERSION     DAOPNH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14401_length_6243_cov_5.008242"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKZ74_10585"
     CDS             complement(<1..227)
                     /locus_tag="PKZ74_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93247.1"
                     /translation="MLNKESIYVILFSIPIYQLLFYTVQLVSFRRKNPSKKYLGLLLL
                     SMTAFLLMNAIHFLGYNDTFAYLYFIYLPVL"
     gene            324..1151
                     /locus_tag="PKZ74_10590"
     CDS             324..1151
                     /locus_tag="PKZ74_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM93248.1"
                     /translation="MEEKTLKTALITGASGGLGRETSNLLLKAGYRVIALDIDSEPLL
                     KNMNQNNLFFRQMDVSDPVGMKLVEEEFRLEQCGLDLLICLAGIYESFPVTEASPEKF
                     MKMMAVNTFSIQIAVNGLLKALITAQGRVIVVSSESYRFQSMFQPYMITKAALEAYCR
                     SARQELALKGVKLSVVRPGAIDTPLLRWMDSSGFVDQYTVFRNELLSAWKKSLSMVGH
                     RSAPSRVAALILKAASSRKPRRIYRINNNPLLKFLNHMPEGLIDRIIIMIIRNESGR"
     gene            complement(1153..2547)
                     /locus_tag="PKZ74_10595"
     CDS             complement(1153..2547)
                     /locus_tag="PKZ74_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM93249.1"
                     /translation="MKKDKSTRRDFLRIAVTGAAALGMGLRNVKGEKIEQFGSVTPWR
                     PSGQSSVMGLVCKPIEKVRIGIIGLGMRGMEAVRRLQYVEGVEITAVCDVLPDRVMAA
                     QKAVSEKGHPVPEGFSEDSLAWMKLCESENVDLVYACTPWDLHTPNAVHAMNNGKHAA
                     IEVPAAMTLDDCWLLVDTAEKTQRHCMMLENCCYDFFELATLNMARQGIFGEIMHAEC
                     AYIHDLRWLKFDKENGYADMWRLKYSMGHNGNLYPTHGLGPVAQIMGINRGDRMEYLT
                     STSTSQVGMSLYAKNTFGEGSPEFNQRYSLGDMNTTMVCTARGKTIMIQHDTTSPRPY
                     SRIHMISGTNGIAMKWPAEQIALEPNAHQWLPDEARDELLTRYEHPLARKMGEMAREV
                     GGHGGMDYIMDWRLIQCLRNGWPLDQDVYDAAAWSSIVELSEISVNDRSRPVEIPDFT
                     RGAWEEAKPLGIVE"
     gene            2687..4633
                     /locus_tag="PKZ74_10600"
     CDS             2687..4633
                     /locus_tag="PKZ74_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPM93250.1"
                     /translation="MIIFSSLMGQSCRKNEAQIHDYPIQPVPFTQVRIMDNFWSKRLE
                     TNHKVTIPIAFHQAEKTGRIDNFRIAGRLKEGKFSSIYPFDDSDVFKNIEAASYSLQL
                     FHDPDLESYLDTLIGYIAAAQEPDGYLYTNRTIDPKNTHEMAGPERWINEEAHSHELY
                     NAGHLYEAAVAHYQATGKRELIDVALRNADLIDNIFGWGKIEKAPGHQEIEIGLVKLF
                     RTTGEVKYLDLAKFFLDVRGRAGEEYNQMHMKVKDQNEARGHAVRAQYMYSAMADIAA
                     LTADTGYLEAIGRIWQDVTEAKTYITGGVGSVRQTEGFGPPFHLPNEEAYCETCAAIA
                     NALWNYRMFLLHGDSKYYDVFEKVIYNGFLSGVSLGGDQFFYPNPLSCEGQCRRKPWF
                     GCACCPVNITRFLPSLPGYTYATYGSVLYINLFIGSAALINLADRRFTIHQETEYPWE
                     GRVTFRVETEQPIVFTMLIRIPGWAKNEAFPTGLYRFADTLAEKPELEINGVKEEIIL
                     DRGYARIERKWSDRDSVVLALPYQPRKVIADQRVEAVRGKTALQCGPVVYCLEDIDQP
                     DSSIIQGKTQGSEVIIFGYDQDLLGGIGTLNGHIQTNDNQENNSSAVIYEKFRAIPYY
                     SWANRDQNKMDVWIGMNKDRKKQE"
     gene            complement(4638..5795)
                     /locus_tag="PKZ74_10605"
     CDS             complement(4638..5795)
                     /locus_tag="PKZ74_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPM93251.1"
                     /translation="MGFISLIYVVFASIALFACVLQLIVTFKKENDLTFLIGAFLSLV
                     VFGKFLFLVLCTTRFGIECEPGLILRISLLFNIAVFICMMGVIAHLFKEKRRIIWLST
                     VALLLLIFLLVLSLPDEILFGSGSTIRKTWLTYGDRVILSGTGGSVWRILLSLSTLLF
                     LGLSGLIFFRHPFKPGRSSFLFILVGSGLIFITGTADALIDLEWVKFSYVMPLGIFSY
                     YLILIFIPFIRFIDEINNQQVFVREEKRYQHLASKAELIVVSLNRTGHVEFINPYFYR
                     LTGFKPEEVIGKDWFEFFIPPKEHYQMQGAFLELLHTENHPRHINPILTKDHKQIMIN
                     WFNVRTQDQSGTITGSLSIGVDISEHVEENRSLKQKLRDAEKLIEKLKAER"
     gene            5965..>6243
                     /locus_tag="PKZ74_10610"
     CDS             5965..>6243
                     /locus_tag="PKZ74_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPM93252.1"
                     /translation="MLSKKAQYSILALVKLAKEYKNGPILISSISDSEKIPKKFLETI
                     LFDFKQTGILASKKGKGGGYYLIRHPREVTLADIIRCIDGAIALIPCVT"
BASE COUNT         1774 a         1390 c         1447 g         1632 t
ORIGIN      
        1 aacaatacag gcagataaat aaaatacaag tatgcaaaag tatcattata acctaaaaaa
       61 tggatggcat tcatgagcag aaacgctgtc atggataata atagcaatcc cagatatttt
      121 tttgacgggt tttttctcct gaatgaaacg agctgaacgg tatagaacag caactggtag
      181 atcggtatgg agaatagaat aacgtaaata ctttctttgt tcagcatgtg gttaattata
      241 aacaggcagg tgcaaagata ttaattttga cactcaggca tggaagaaac cggtttataa
      301 acctaaatca accttaacat attttggaag aaaagacttt aaaaactgct ctcatcaccg
      361 gagcttcggg aggattggga agggaaacat caaaccttct gctgaaagcg ggttacaggg
      421 ttattgccct ggatatcgat tccgaaccgc tgttgaagaa tatgaaccag aataatttgt
      481 tcttccggca gatggatgtc tcggacccgg tcgggatgaa actcgtggaa gaagaattca
      541 gacttgaaca atgcggttta gatttattga tttgcctggc aggcatttac gaatcgttcc
      601 cggtcaccga agcatctccg gagaaattca tgaaaatgat ggctgttaac acatttagta
      661 ttcaaatagc tgtcaatgga ttattaaaag cattgattac agcccaaggt cgtgttatcg
      721 tggtcagcag cgaaagctac cgtttccaga gcatgttcca gccatacatg atcacaaagg
      781 cggcgctaga agcatactgc aggagcgccc ggcaggagct cgccctgaaa ggtgtcaaat
      841 tatctgtggt ccggccgggt gctatagata caccgttatt acgatggatg gactcctccg
      901 ggttcgttga tcaatatact gttttcagaa acgaattgct ttcggcatgg aaaaagagcc
      961 tttcgatggt aggtcacaga tcggcaccat ccagggtggc agcgttaatc ctcaaagccg
     1021 cttcttcaag gaaacccagg aggatataca ggatcaataa caatcccttg ctgaaatttt
     1081 taaatcatat gccggaaggc cttatcgacc gtataattat aatgattatc cgcaacgagt
     1141 cgggaagatg atctattcta ctatgccgag gggctttgct tcctcccagg cacccctggt
     1201 aaaatcaggt atctccaccg gccggcttct gtcgttgacc gagatctcgc tgagttcaac
     1261 gatacttgac caggcagcgg cgtcatacac atcctggtcg agaggccatc cgtttctcag
     1321 gcactggatc agccgccagt ccatgatata gtccattccg ccgtgtcctc ccacttcgcg
     1381 ggccatctcc cccattttcc tggctaaagg gtgttcgtag cgtgtcagca gttcatctct
     1441 ggcttcatcc ggaagccact gatgggcatt gggttcgagg gcgatctgtt ctgcaggcca
     1501 tttcatggcg atcccgttag tcccgctgat catgtggata cggctgtatg gcctggggct
     1561 ggtagtatca tgctggatca taatggtttt gcctctggct gtgcagacca tggttgtatt
     1621 catatcgccc agcgaatagc gctgattgaa ttccggtgag ccttcaccga atgtattctt
     1681 cgcataaagg ctcattccca cctggctggt ggatgtcgac gtcagatatt ccatccggtc
     1741 gcccctgtta atccccatga tttgggcaac cggtccaaga ccgtgtgtcg gataaaggtt
     1801 tccattatgc cccatactgt atttcaatct ccacatgtcc gcatagccgt tctccttatc
     1861 gaatttcagc catctgagat catggatata ggcgcattcg gcatgcataa tttcaccgaa
     1921 tatgccctgg cgggccatgt tgagggtggc aagttcaaaa aaatcgtaac agcagttttc
     1981 cagcatcatg caatggcgtt gcgttttttc cgctgtatct accaacagcc agcaatcatc
     2041 cagtgtcatg gctgcgggaa cctcaatggc ggcatgttta ccgttattca tagcatgaac
     2101 agcgttgggc gtatggagat cccatggcgt gcaagcataa accaaatcga cattttccga
     2161 ttcgcaaagt ttcatccagg caagggaatc ctccgaaaaa ccttccggta caggatgtcc
     2221 cttttctgaa acggctttct gcgccgccat aaccctgtcg ggcagaacat cgcaaacagc
     2281 ggttatttca acgccttcca catactgtaa tcgcctgacc gcttccattc cgcgcatccc
     2341 cagcccgatg atgccgatcc ggactttttc gattggcttg cagaccagtc ccataacaga
     2401 actctgcccg gatggcctcc agggtgtaac agaaccgaat tgttctatct tttctccttt
     2461 aacgtttcgg agtcccattc ccagtgcagc cgcaccggtc actgcgattc gcagaaaatc
     2521 ccttcgtgtc gatttgtcct ttttcatgat cctggatttt tccggtttga ttaagtttgt
     2581 aaatttataa aatgtaagag gaggtcattt cacctttaaa aaaattatca tgaatatgag
     2641 gtatttggct ttgagcagaa aattgttcaa ttgttcgatt gttctggtga taattttcag
     2701 ttcgctgatg ggtcagtcgt gccgtaagaa tgaagctcag atccatgatt acccgattca
     2761 gccggtaccg ttcacgcagg taaggataat ggataatttc tggtcaaagc ggctggaaac
     2821 caatcataag gtcacgattc ccatcgcttt tcatcaagca gagaaaacag gaaggattga
     2881 caatttcagg attgccggcc ggttaaaaga gggcaaattc agctcaatat accctttcga
     2941 cgattcggat gtttttaaaa acatcgaagc agccagctat tcccttcagc tttttcacga
     3001 tcctgattta gaatcctatc ttgacaccct gattggttat attgctgcag cccaggagcc
     3061 tgatgggtat ctttacacca accggacgat cgatccgaaa aatacgcatg aaatggccgg
     3121 gccggaaagg tggatcaacg aagaggccca cagccacgag ctttacaacg ccggccacct
     3181 ttatgaagct gccgtcgccc attaccaggc caccggtaaa agggaattga ttgatgtcgc
     3241 attgcgaaat gctgatttaa tcgataatat tttcggttgg ggtaagatag aaaaagcgcc
     3301 cggtcaccag gagatagaga tcgggcttgt gaaattgttc cgtacgaccg gagaagtgaa
     3361 ataccttgat ctggcgaagt tttttctgga tgtcagggga cgagcaggag aggaatacaa
     3421 ccagatgcat atgaaagtga aagatcagaa tgaagccagg ggacatgcag tgcgggcgca
     3481 gtatatgtat tcggcgatgg ctgatattgc tgccctgaca gccgataccg gttatttgga
     3541 agctattggc cggatatggc aggatgttac cgaggccaag acttatatta ccggtggtgt
     3601 gggttcggtc aggcagaccg aaggattcgg ccctccgttc catcttccca acgaggaagc
     3661 ttattgtgaa acatgcgctg ctatagccaa tgcattatgg aactaccgga tgtttctcct
     3721 gcatggtgat tcaaaatatt acgatgtttt cgagaaagtg atatacaatg gctttctgtc
     3781 cggagtttca ctcggcggcg accaattttt ttatccgaac ccgctttcgt gtgaaggcca
     3841 gtgccggcga aaaccctggt tcggctgtgc ctgctgcccg gtcaacatta cccggttcct
     3901 gccatcactg ccgggttaca catatgcaac atatggcagt gtattatata tcaatttatt
     3961 tatcggcagc gccgctttga taaatctggc tgatcgtcga tttacaatcc accaggaaac
     4021 ggaatatcca tgggagggcc gcgtgacatt ccgggttgaa accgagcaac ccatagtttt
     4081 caccatgctg atcaggattc ccggatgggc aaaaaatgaa gcgttcccca ccgggcttta
     4141 tcggtttgcc gacacactgg cagaaaagcc ggagctggag atcaacggag tgaaagaaga
     4201 aataatcctg gatcggggat atgcacgtat cgaaaggaaa tggtcggacc gggattctgt
     4261 cgttttagca ctcccttatc agccacgcaa ggttattgca gaccagcggg ttgaagccgt
     4321 ccgggggaaa acagcgcttc aatgcgggcc ggtagtttat tgcctggagg atatcgacca
     4381 gccagattca tcgataatac aaggtaaaac tcaaggatca gaagtcatta tattcgggta
     4441 cgatcaggac ctgctcggcg ggatcggaac gttaaacggg catatccaaa ctaatgataa
     4501 ccaggagaat aactcaagtg ctgttattta cgagaagttc agggccattc cctattacag
     4561 ttgggcaaac agggatcaaa acaaaatgga tgtctggatt ggaatgaaca aggacaggaa
     4621 aaagcaggaa tagcctgtta tcgttcggct tttaatttct caatcagctt ttcggcatcc
     4681 cggagtttct gtttcagcga ccggttttcc tcaacatgtt cagagatatc aacacctata
     4741 ctcaggctac ctgtaatggt ccctgattgg tcctgggttc tgacattaaa ccaattgatc
     4801 ataatttgct tatgatcttt cgtcagaatc ggattgatat gccgtgggtg attttctgtg
     4861 tgaagcaact ctaaaaatgc cccctgcatt tggtaatgtt ctttaggagg aataaaaaac
     4921 tcaaaccagt ctttgccgat tacctcttcc ggtttaaaac ctgtcagtct gtaaaaatat
     4981 gggttgataa attccacatg ccctgtacga tttaaggaaa cgactatcaa ttcagcctta
     5041 ctggcaagat gctgatatct cttctcttcc ctgacaaaga cctgctggtt attgatctcg
     5101 tctataaatc ttatgaatgg aataaaaatc agaataagat aataagaaaa tataccgagt
     5161 ggcatcacat aggagaattt cacccattca aggtcgataa gggcatctgc cgttccggtg
     5221 ataaaaatca gcccgctgcc tacgaggata aacaaaaatg aacttctgcc gggtttaaaa
     5281 ggatgacgaa agaaaataag gccgctaagg ccaaggaaaa gtaaggtaga aaggctcagt
     5341 aatatcctcc aaaccgaacc acccgtacct gacaggatga ctctgtcgcc atacgtaagc
     5401 catgttttcc ggatggtact gccactgccg aaaaggattt catccggcaa tgaaaggaca
     5461 agcaggaaga ttaaaagcag gagtgcaacg gttgaaagcc agatgattct tcgtttttct
     5521 ttgaagagat gcgctataac ccccatcatg catataaaaa cggcaatatt gaaaagcagt
     5581 gagatcctga gaatcaaacc cggttcacat tcaataccaa aacgggtcgt gcataaaacc
     5641 aggaatagaa atttaccaaa aacaaccagg gaaaggaatg ctccgatgag aaaagtcagg
     5701 tcattttctt tcttgaatgt gacaataagc tgaaggacac acgcaaatag cgcaatggaa
     5761 gcaaaaacca catatatgag cgagataaaa cccatttcca aataaacgaa gaattggcaa
     5821 aactgtgaca aagttaaaca aatgattgtc gctagtcaag tatttattac ttttacaatc
     5881 gtatacttta ttatactgta tttaattaac taattaataa taagccatat tccttcaatt
     5941 atggaatttt ggtttttacg atagatgtta agtaaaaaag cccaatattc tattctggcc
     6001 ctggtaaaac tcgctaagga gtataaaaac ggtcccattt taattagcag tatatcggat
     6061 agtgaaaaaa ttcccaaaaa gttccttgaa acaattttgt ttgatttcaa acaaaccgga
     6121 attctggcca gcaaaaaggg caaaggcgga ggatattatt taatccggca tccccgggaa
     6181 gtaacgctgg cagatataat aaggtgtata gacggcgcca tcgcgctcat tccttgtgta
     6241 acc
//
LOCUS       DAOPNH010000206         6197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14543_length_6197_cov_4.888961, whole genome shotgun sequence.
ACCESSION   DAOPNH010000206 DAOPNH010000000
VERSION     DAOPNH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14543_length_6197_cov_4.888961"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKZ74_10615"
     CDS             complement(<1..915)
                     /locus_tag="PKZ74_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93253.1"
                     /translation="MARFTIIFLLLCLTGQAFSQINPADSILQTYTSLPDDSTKVNTL
                     ISVAGKYVRIDPTVTILVSSEALRIAEQIRYQKGIGYALKNIGMGYYFKADYLEVLTY
                     WQRSLNTFDSIGEKNGVANMSSNLGAVHFSQGDDSKAIEYYLKSLKVSEETGDKLRIA
                     TALINIGAVYTNKVQTYDMALQYYYRALPLATALEDYEAIGTVAVNMGEIFMKQNISD
                     SALYYFNKSLEAYEIAGSVKVSYTLNNLGKVYTQQKDYTKAVFVQKQAVERARKVDAR
                     LEMTQSLVGLAKTYKAQGDLTQAISAFRE"
     gene            complement(933..1169)
                     /locus_tag="PKZ74_10620"
     CDS             complement(933..1169)
                     /locus_tag="PKZ74_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93254.1"
                     /translation="MEPQHNVFGWVEVPVSDMATDGTLVYFTAFSGDLNIELGRVEKA
                     GGKVLQEKTLIAEGYGQMALILDTEGNRIALHSR"
     gene            complement(1305..1377)
                     /locus_tag="PKZ74_10625"
     tRNA            complement(1305..1377)
                     /locus_tag="PKZ74_10625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1342..1344),aa:Arg,seq:tct)
     gene            complement(1428..3020)
                     /locus_tag="PKZ74_10630"
     CDS             complement(1428..3020)
                     /locus_tag="PKZ74_10630"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HPM93255.1"
                     /translation="MYLCKLEIENFRGIRFARMNLNETTVVTGENDSGKTTLLDAVCQ
                     VLSPSGESHGIIFTAGDFHMRSEQPEFRPSGSIGIRLTFRERRPDEWSSLRLNDYGFR
                     LEDDQETLQELTLEVKSGVLTERGEARASWRIRTAGQTEMAFSEDMAILDWFRRLNPV
                     FRIRSDMFSKANLDFSREPGIFEDFRTSIKYVESHSNIFRSDGIPMEDIIYDILGKKS
                     DSNSGETRRIVLHHGSAAEKIGMLVFIEAFLESGGLKADASAEPIIIIEDPEAHLHPM
                     TLQSVKILIERMNWQKIITTHSGSLLSDFALEDIRRITRREGNIREYRVKPGSLSSEE
                     LRRLSYHVRKMIPNATFARCWLLVEGESEMWLMPHLARLCGYDFAMEGVVCVEFAQCG
                     IAPLVKAASQLGIEWFLMADGDAAGRAYIETARHFARQLNREPEEHCLRFREKDIENH
                     LFFNGYADIYSQYSGIPVHDSENMQPRRIIGRAIHRNSKPFMAIAIIEAMARTGSQGV
                     PSELRKVVEQCVGLAGGSENSR"
     gene            3113..3643
                     /locus_tag="PKZ74_10635"
     CDS             3113..3643
                     /locus_tag="PKZ74_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93256.1"
                     /translation="MKRLIIKLLALSFMLTISISSFSQTDENMKAMVAKMNDEMMELL
                     KAGRYEAMAKYYDINLISMPNYRVMEKGWQLILNNNLGRKKGGYQVADGKKTTTDLII
                     GEDMMVDLGQYTLTINFPGLEKPKVDNGKYMNVWRKGKDGSWRIVVETWNADKSPNAP
                     AQPKPTMQPAGQPVKK"
     gene            3711..4295
                     /locus_tag="PKZ74_10640"
     CDS             3711..4295
                     /locus_tag="PKZ74_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPM93257.1"
                     /translation="MNNPARIREAIKDSIGVKEILAADPEMIRTIEMIAETCSRALCD
                     GQKIMFCGNGGSAADAQHLAAELSGRFYFDRKPMAAEALTVNTSYLTAVSNDYSFEDV
                     FSRLVEGSGRPGDILVGLSTSGNSENVVRAFAKAHESGIITVALTGEDGGKLAPLADY
                     LICVPSADTPRIQESHILIGHIICELIENHLFRV"
     gene            4292..5002
                     /locus_tag="PKZ74_10645"
     CDS             4292..5002
                     /locus_tag="PKZ74_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM93258.1"
                     /translation="MTGEAIILAGGYGTRLKGVIPDGPKAMAPINGRPFLEYVMDYLD
                     RWGLNHIVLSVGYQQEKIINHFGTGYKSLAIDYALEDEPLGTGGAILNALPLIKGFSS
                     FIFNGDTYFDVNLQRLDDFRRIKEADLCLAIRFEIDPGRFGVLEFDNNNRITGFNEKS
                     ADIEEGYINGGVYILRKDYLLRFGLPRKFSFENDFLQKYHSTEEFYGMRCFSYFRDIG
                     IPEDYNKAVDEFKRLIFY"
     gene            5013..5555
                     /locus_tag="PKZ74_10650"
     CDS             5013..5555
                     /locus_tag="PKZ74_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPM93259.1"
                     /translation="MFLDRDGVISERLPGDYVRKWEDFRFIGGVFESLRMFSGIFGRI
                     IIISNQQGVGKGLMTAEELHEIDREMKLEIGNSGGRIDAAFYCTSLASENNPDRKPGV
                     GMGLKAKALYPEIDFSKSVMVGDSDSDMEFGKKLGMVTVYIGSDKNCLENADYVFDSM
                     FSFAQSINESHHREHRFTQS"
     gene            5561..>6197
                     /locus_tag="PKZ74_10655"
     CDS             5561..>6197
                     /locus_tag="PKZ74_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPM93260.1"
                     /translation="MTPSLILIIFLLYTALLFFITWLTARKATNRSFYIGNKESPWIV
                     VAYGMIGASLSGVTFMSVPGWVGDTQFSYMMVVLGYLIGYFIIATVLMPLYYRLNLTS
                     IYSYLEKRFGFWSYKTGAFFFLMSRTIGASFRMFLVVNVLQVFVFDAWGIPFIATTLI
                     FIILILLYTFKGGIKTIIWTDTVQTTFMLTALVITIYLVTREMNMDFRQMFN"
BASE COUNT         1692 a         1428 c         1415 g         1662 t
ORIGIN      
        1 ttcacggaat gctgaaatag cctgggtaag atccccctgt gccttatagg tcttggcaag
       61 cccgaccagc gattgtgtca tctcaagccg ggcatctact tttctggctc tttcaactgc
      121 ctgtttctga acaaataccg ctttagtata gtccttctgc tgtgtatata ctttacccaa
      181 attgttcagc gtgtaggaaa cttttacact cccggcaatt tcataagctt caagcgattt
      241 gttaaagtag tataatgcgg agtcggaaat attttgtttc atgaaaattt cacccatatt
      301 cacggcaacg gtgccgatag cctcgtaatc ttcaagcgcg gtggccagtg gcagggcacg
      361 gtagtagtac tgcaaggcca tgtcataggt ctgaaccttg tttgtatata cagctccaat
      421 atttataagc gctgtggcta tccggagttt gtcgccggtc tcttccgaga ctttcagcga
      481 tttaagatag tattcgatag ccttggaatc gtcgccctgg ctgaaatgta ccgcgcccag
      541 attgcttgac atattcgcca cgccgttttt ctcgccgatg gaatcaaatg tattcaggga
      601 acgttgccag taagtaagca cttcaaggta gtctgccttg aaatagtatc ccatgccgat
      661 attttttaag gcgtatccga tacctttttg atagcggatc tgctcggcaa tgcgcaatgc
      721 ttcagaactt acaaggatag tgaccgtcgg atcgattctg acatatttgc cggcaacaga
      781 aatcagggtg tttacttttg ttgaatcgtc aggtaatgat gtataagttt gtaagatgct
      841 gtctgccggg ttaatctgtg aaaaagcctg gcccgtaaga caaagcaaca ggaaaataat
      901 ggtaaatctc gccatctaga tcgtataatt gactacctgg aatgaagagc tatccggtta
      961 ccttccgtat ccaggatcag cgccatttgg ccatacccct cagcgatcag tgttttctcc
     1021 tgcaaaacct ttccaccggc tttctcgacg cgacccagtt caatgttaag atcccctgaa
     1081 aaagcagtaa aataaactaa tgtgccatcc gtggccatat cactgacagg cacttcaacc
     1141 catccaaaaa cattatgctg tggttccata ataaatgcgt ttgtgaggta acaaagagaa
     1201 aggcaaattg tttaaaaatt gagggaaaga atctctaaaa ataaaagggg attacaacta
     1261 agaagaaata ttaacccaac actaataacc caactctcaa cctgtgatcc cggaaggatt
     1321 cgaaccttcg acccacagct tagaaggctg ttgctctatc cactgagcta cgagaccgtt
     1381 ttgcgggtgc aaagatacat cttttttccc cgactaacga ccaacggcta acgactattt
     1441 tccgacccgc ctgccaaccc gacgcactgt tctaccacct ttctgagttc tgatggcacc
     1501 ccctgtgatc cggtccgggc catcgcctcg ataatcgcaa tagccataaa cggtttggaa
     1561 ttacgatgga tcgcccggcc gataatcctt ctgggctgca tattttcgct gtcatgtacc
     1621 gggatgcccg aatattggga atagatgtca gcataaccgt taaagaacaa atgattctca
     1681 atatctttct ccctgaacct cagacaatgc tcttcaggct cacggtttaa ctgccgggca
     1741 aaatgcctgg cggtttcaat gtaagccctt ccggcggcat ctccatcggc catcaggaac
     1801 cattcaatgc ctagctgtga ggcggctttt accagtgggg ctatgccgca ctgggcaaat
     1861 tccacacaaa ccaccccctc catggcaaaa tcatagccgc ataacctcgc caggtgaggc
     1921 atcagccaca tctccgattc cccttcaacc agcaaccagc agcgggcaaa agtggcattc
     1981 gggatcattt tcctgacatg ataactcagc cggcgcaatt cttcactgct caggcttccg
     2041 ggtttgaccc ggtattcccg gatatttccc tctctgcggg taatgcgccg gatatcctcc
     2101 agggcaaaat ccgataacag gctgccggaa tgcgtggtaa tgatcttttg ccagttcatc
     2161 cgttcaatca atatcttgac agattgtaac gtcatcggat gtaaatgggc ctcggggtct
     2221 tcgatgataa tgataggttc ggcagatgca tccgctttca atccgcctga ttcaaggaat
     2281 gcttcgataa ataccagcat tccgatcttt tccgcagcag aaccatgatg cagaacgatc
     2341 cggcgtgttt caccactatt tgaatccgat tttttaccca ggatatcata aataatatcc
     2401 tccatgggaa taccatcgct ccggaatata ttggaatggc tctcaacgta tttgatggaa
     2461 gtccgaaaat cttcaaaaat accgggctca cgggaaaaat ccagattggc tttcgaaaac
     2521 atatcgctcc tgatcctgaa taccggattt agcctgcgga accaatccag gattgccata
     2581 tcctccgaaa aggccatttc agtttgcccg gctgttctga tgcgccatga ggccctggct
     2641 tcaccccgct ccgtcagaac acccgatttc acctcaagag tcagctcctg cagggtttcc
     2701 tgatcatcct ccagcctgaa cccataatca ttcaaacgta aagatgacca ctcatccggc
     2761 cttctttccc tgaaagtaag ccggataccg atcgaaccgg aaggcctgaa ctccggttgc
     2821 tcagatcgca tgtgaaaatc accggctgtg aaaatgatgc catggctttc accggaaggc
     2881 gaaagtacct ggcaaacagc gtccagcaag gtcgttttac ccgaatcgtt ttccccggtg
     2941 accaccgtcg tctcatttaa attcatccgt gcaaaacgaa ttccacgaaa attctcaatt
     3001 tcaagtttgc ataagtacat gcatcaaaat taaaactatt ttttcgcgtt ttaaattatc
     3061 ctatctttgg aaccgtttta aataattatg aagactaaaa taatcctgat gtatgaaaag
     3121 actcataatt aagcttttag ctttatcatt catgctaacg atctcaataa gttcattcag
     3181 tcagaccgat gaaaatatga aagccatggt ggcaaagatg aatgacgaga tgatggaatt
     3241 actgaaagcg ggaaggtatg aagcgatggc caaatattat gacataaacc tgatctcaat
     3301 gcctaattac agggttatgg aaaaaggttg gcaattgatc ctgaacaata atcttggacg
     3361 taagaaagga ggataccagg tagctgacgg taaaaaaacc acaactgacc tgataatcgg
     3421 tgaagatatg atggtcgacc ttggccagta tacgctgact attaattttc ccgggttgga
     3481 gaagccaaag gtggataatg gaaaatacat gaatgtttgg aggaagggta aagatggttc
     3541 ctggcggatt gttgtggaaa cctggaatgc cgataagagc ccgaatgctc ccgctcaacc
     3601 gaaaccgacc atgcagccgg caggtcaacc agtgaaaaaa tagccgaccc tctttcaacc
     3661 gctttgcatt ataaaacaat aaaccagtct ttcagccttt gaaaccagaa atgaataatc
     3721 cagcccggat aagagaggca attaaagatt cgatcggggt taaagagata ctcgctgctg
     3781 accctgaaat gatccggacc attgaaatga ttgcagaaac gtgttcacga gccctgtgtg
     3841 acggtcagaa gatcatgttt tgcggcaacg gtggaagtgc tgcagatgct cagcacctcg
     3901 cggctgagct ttcaggacgg ttctatttcg acaggaaacc aatggcggcg gaagcactaa
     3961 cggtcaatac atcttatttg acggctgttt caaacgatta tagttttgaa gatgtttttt
     4021 cgcgcctggt tgaaggttca ggacggcccg gagacatcct ggtaggcctt tccacctccg
     4081 ggaattcgga aaacgtggtt agggcctttg ccaaagccca tgagagcgga ataattactg
     4141 tggcactgac cggtgaagat ggcggaaaac tggctccatt agccgattac ctgatatgcg
     4201 tcccttctgc tgatacgccc aggatacagg aatcccatat cctgatcggg catatcatct
     4261 gcgaattgat cgaaaaccac cttttccggg tatgacagga gaagccatta ttcttgccgg
     4321 tggttacggt acaaggttga aaggggtcat acccgacggc ccgaaagcca tggcgcccat
     4381 caacggcaga ccttttctgg aatatgtcat ggactatctc gaccggtggg gactcaacca
     4441 catcgtcttg tccgtcggat atcagcagga aaagatcata aatcatttcg gaacaggata
     4501 taaatcactg gcgattgact atgccctgga agatgaaccg ctcggaaccg gcggcgccat
     4561 cctgaatgcc ctgccactta taaaaggttt ttcaagtttc atttttaacg gcgacaccta
     4621 ttttgatgtc aacctgcaac gcctcgatga tttcaggcgt atcaaagaag ccgacctgtg
     4681 ccttgctatc cgttttgaaa ttgatccggg acggttcgga gtgctggaat ttgacaataa
     4741 caaccggatt accggtttca atgagaaatc cgctgatatt gaagaaggtt atattaacgg
     4801 cggggtctac attttgagaa aagactatct gctcaggttt ggattgcccc gcaaattcag
     4861 tttcgaaaac gactttctcc agaaatacca ttcaacagag gaattctacg gtatgagatg
     4921 tttctcttat ttcagggata ttggcatacc ggaggattat aacaaagcag tcgatgaatt
     4981 caaaaggctt attttttact aaggattgga ccttgttcct tgaccgcgat ggtgttataa
     5041 gtgaacgcct tcccggtgat tatgtcagga agtgggaaga tttccgtttt atcggcggcg
     5101 tttttgaatc tttgagaatg ttttcaggga tattcggcag gatcatcatt ataagtaatc
     5161 agcagggagt ggggaaagga ttgatgacgg cggaggaatt gcatgaaatc gaccgggaaa
     5221 tgaaattgga aatcggaaat tcggggggga ggatcgatgc ggcattctat tgtaccagtt
     5281 tggcttctga aaacaatcct gaccggaaac caggggtggg gatggggctc aaggcaaagg
     5341 ccctttaccc ggagatcgat ttctcgaaat cagtgatggt aggggatagt gatagcgata
     5401 tggaatttgg gaaaaaactc ggaatggtaa cggtatatat tggatccgac aaaaactgtc
     5461 ttgaaaatgc agactatgtt tttgactcca tgttttcttt tgcgcaatcg ataaatgaat
     5521 ctcaccacag agaacacaga tttacacaga gttaacacag atgacgccat cactgatatt
     5581 aatcattttt ctgctctata ccgcgctgct gttttttatc acgtggctca ctgccagaaa
     5641 ggctacaaac aggtcattct atattggtaa taaagaatcg ccctggatag tggtggccta
     5701 tggcatgatt ggcgcatccc tgtcgggggt gacttttatg tcggtgccgg gctgggtggg
     5761 agatacccag ttttcctata tgatggtcgt attgggatac ctgatcggtt atttcatcat
     5821 tgcgaccgtt ttgatgccac tgtattacag gctgaacctc acttccattt attcgtacct
     5881 cgaaaaaagg tttggcttct ggtcgtataa gacaggggca ttttttttcc tgatgtcgcg
     5941 cactatcggg gcttccttca ggatgttcct ggtagtcaat gtactccagg tatttgtgtt
     6001 tgatgcctgg ggcatacctt ttatagccac aacactcatt tttatcattc ttatcctgct
     6061 ctatactttt aaaggcggta taaaaacgat tatctggacc gatactgtcc aaacgacatt
     6121 catgcttaca gccctggtca ttaccatata tcttgttaca cgtgagatga atatggattt
     6181 caggcaaatg ttcaata
//
LOCUS       DAOPNH010000207         6164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14651_length_6164_cov_5.664757, whole genome shotgun sequence.
ACCESSION   DAOPNH010000207 DAOPNH010000000
VERSION     DAOPNH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14651_length_6164_cov_5.664757"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PKZ74_10660"
     CDS             complement(<1..1472)
                     /locus_tag="PKZ74_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93261.1"
                     /translation="MKNPALPHLLTTLITLITFITLITFTTLTTHAQWSTDPAINNAI
                     IALSGEEAIPKIATCSNGDTYIASFSNNGNYNVRMQRLNAQGIPQWADDGILISDNPQ
                     DSWLTDWDICADNANHAILVFPDIRNGGANVVAYRISPTGTFAWGDDGIVLSTGDANY
                     SPKVTATAAGNAIVAWMQDDVIRVQKISPTGSLPWGSGGLVLQQTGITFSWPQLLPVG
                     SDEFIMKYYQDSGTFPAITRHIYAQRYNAGGTGVWSSPTVISNAGGIAAWTQVLPFIN
                     DGSDGFYIAWHDDRDNNMLSSTWVNHVNSSGAVLFTANGVEASTLGGRNHFYPQLALP
                     PGSSNVFVFWNEMDGDQNNRGIYGQKITSTGSKLWATTGMIFIEISPVDVYPEAARHS
                     PDDVVVFYSEQVSGLDYTLKAFRIDADGGYIWTPNHKTLCSVVSEKVHVETNDFADNQ
                     WIMAWEDDRAGQKDIYAQNIQLDGSLGPYDPNEGTIEGVVT"
     gene            complement(1705..2358)
                     /locus_tag="PKZ74_10665"
     CDS             complement(1705..2358)
                     /locus_tag="PKZ74_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPM93262.1"
                     /translation="MPSHKIINIRPAILISCLAVLMFSPMFVLRQVGPIDFWWWMTIN
                     LSILISLSIITDWEYYYILSKDFTKSILKKIMIGLISAMVLYFVFFLGNYFSRLWFDF
                     AGPGIENVYKFKGDAAGIRIAILMLLIIGPGEELFWRGYLQRKFSKKYGKWTGLIIAL
                     ILYTGVHIFTGNFMLIMAAFICGLFWAWMYLKYESMLINVISHTAWDIVVFLLLPFH"
     gene            complement(2440..3399)
                     /gene="menA"
                     /locus_tag="PKZ74_10670"
     CDS             complement(2440..3399)
                     /gene="menA"
                     /locus_tag="PKZ74_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HPM93263.1"
                     /translation="MEDQAKLSFFKKWWISARPFSFPASTMPVIFGTVLAYVYGGHEF
                     KPLLFILAFIAMVILHSGANILSDIFDFEKGLDKVPGPTSGGVVRGIISIREAWIGSV
                     SMLVFGTIIGLYLTWVSGPWLLAIGILGLLVGTFYTSHSMVNMKYNGLGDLAVFLNFG
                     ILGSLGAYYVQSGALSWIPVIWAIPMSMLVIAILHANNWRDIQSDKEIKIFTIASLLG
                     DKNSLRYYGVLIYGPFIIIVGLILVPFFLFPNMTAMPFTFLITLAALPLAFRVWKKAL
                     NRKTPAQPLDFIALDGASAQLNLLFGSLCTLALILQAVIDWAK"
     gene            complement(3495..6062)
                     /locus_tag="PKZ74_10675"
     CDS             complement(3495..6062)
                     /locus_tag="PKZ74_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93264.1"
                     /translation="MKSKSLHIAYLLIFLTLFTHPAHSQLEAWGDTTICQGGSAQLFA
                     NGDGVSYFWSSYPTDPSLLIPQQQNPVVSPQVSTMYVVQSNIATGNLILNGSFELGVM
                     GFNSEYVNNQVSIVAEGTYAVVNDAHTVHPNFYCNEDHTSGSGKFMAVNGAGVANVKV
                     WYLSLNNIQPGSKYEFSTWITSLHATNPAILQFSINGELMGEPFQAYPYTCDWYQFFH
                     IWEAGDNTQATISIVNQNTILSGNDFALDDISFATVLVYFDTVYVEVLPQFTSTFVAP
                     QAACTREIVTVTYQGNAPDTAIFNWDFGDASVLSGNGPGPYEIVHNSIGNNTISLWVE
                     GDGCASDTATAGVLVGESPVVAATADAPIIPYGSSTTLHGSYSGGTGPYAWSWEPAAL
                     LTDPGSLNPQTNALFATTGFILAVTDQAANCTGYDTVIIQVTGGPLGVNIAADPEAVC
                     IGQQTTLTAIPYGGTENYSYSWSSSPPGFTSDLPEVTVQPATTTTYSVSVSDGLSEMT
                     ASVTITVHPNPQPDAGPDQLIPYGTNTTLSATASGGSGSYAYHWEPAALVSDPDIPVT
                     QTQNLIIATVFTLTVTDLETGCVSNPDPITISIDGGPLAVVITSDKPKICRGEEAVLT
                     AHASGGNQGNYIYSWSDNLGNSYPSLAQITVSPEDTTVFHVSVDDGYNVNGYDFTLVV
                     FPSAEFGWADGSDHVFACPNDSVLLQPVPRDPSWNFLWSNGATTPEIFVTVTGIGFSL
                     QDYSLTTTTVDGCTFTDTILVSFDFSYCSGIEDEGETTVMISPNPASGAFKVSWKKPG
                     DFEAIRVYNGLGKMISQKETGHYNHVIFDHFKYSPGLYYVHLIGAKSSETRKVLLMP"
BASE COUNT         1611 a         1436 c         1714 g         1403 t
ORIGIN      
        1 gtcactactc cttcaatggt cccttcattg ggatcgtaag gcccgagcga tccgtcgagc
       61 tggatgttct gtgcataaat gtctttctgt cctgcccggt cgtcttccca ggccatgatc
      121 cactggttat cggcaaaatc attggtttcc acatgaactt tttcggaaac gaccgaacaa
      181 agtgttttat ggttgggcgt ccagatatat ccgccatctg catcgatccg gaaagcttta
      241 agggtataat ccaatccgga cacctgttcg gagtagaaaa caaccacatc gtccggggaa
      301 tggcgtgctg cttccggata gacatcgacc ggagaaattt cgatgaagat catcccggtc
      361 gtcgcccata gttttgaacc tgtagaagtg atcttctgcc cgtaaatccc gcggttgttc
      421 tggtcgccgt ccatttcgtt ccagaaaacg aacacattgg aagaaccggg aggcagggca
      481 agctggggat aaaaatggtt gcgcccgccg agggtggagg cttccacccc gttagccgtg
      541 aacagcacag cgccggaact attgacatgg ttgacccagg tgctggagag catattgttg
      601 tcacggtcgt cgtgccatgc aatgtagaat ccgtcggagc cgtcgttgat gaaaggcagt
      661 acctgggtcc aggcggcgat acctcccgca ttggatatca cggtggggga tgaccagacg
      721 ccggtaccgc cagcgttgta gcgctgtgca tagatatgcc gggtgatggc cgggaaagta
      781 cccgaatcct ggtaatattt catgatgaat tcatcgctgc ccaccggcag gagctgtggc
      841 caggagaaag tgatcccggt ctgttgaagg accagtcctc ccgatcccca gggaagggac
      901 ccggtcgggc tgatcttctg cacccggatc acatcgtcct gcatccaggc cacgatggca
      961 tttccggctg cggtagccgt aaccttcggc gagtaattgg catcgccggt agagaggacg
     1021 ataccgtcgt cgccccaggc gaaagtaccg gttggcgaaa tccggtaggc tacgacattg
     1081 gcaccgccgt tacggatatc cggaaaaaca agaatagcgt ggttggcgtt atcagcgcag
     1141 atatcccagt cggtgagcca gctgtcctgc gggttgtcgc tgatgaggat gccgtcatcg
     1201 gcccactgtg gtataccctg ggcgttgagc cgttgcatcc gtacgttgta atttccgttg
     1261 ttggaaaagg aagcaatata ggtatcgccg ttggaacagg tcgcgatctt cggaatggct
     1321 tcttcccctg acaaagcgat aatggcgtta ttgatggcgg ggtcggtgct ccactgggca
     1381 tgtgtggtaa gggtggtaaa ggttataagg gttataaagg ttataagggt tataagggtg
     1441 gtaaggagat ggggtaatgc tggatttttc atgggttaag gtattgaatt gtctgtaaaa
     1501 atacatgatt caacactgtt aatgttcaat tatcctgaaa aaatcacata agaaacccat
     1561 aaaaatacca tggaacaaaa gacaaagaca atagaaatag aaatagaaca atagaacaat
     1621 agaacaatag aacaacagaa caatagaaca acagaacaat agaacaatag aacaattgaa
     1681 cactggaaca acagaaccct gaacctagtg gaacggcagc aagaggaaca ccacgatatc
     1741 ccaggcggta tggctgatca cgttgataag catcgactca tatttcaggt acatccaggc
     1801 ccagaagagg ccgcagatga aggctgccat gatcagcatg aaattgccgg tgaagatatg
     1861 gacgccggta taaaggataa gggcgatgat cagaccggtc catttaccgt atttttttga
     1921 gaattttctt tgaagatatc ccctccagaa aagttcttcg ccgggaccga tgatcagcag
     1981 catcaggatg gcaatccgga ttccggcggc atctcccttg aatttatata cgttttcgat
     2041 tcccgggccg gcgaaatcga accagaggcg cgaaaaataa tttccgagga aaaatacgaa
     2101 gtacaggacc atggcggaga taagcccgat cattattttc ttcaaaatgc tcttcgtgaa
     2161 atctttcgat aagatgtaat aatattccca gtcggtgata atcgacaaac taatgaggat
     2221 tgacaggttg atcgtcatcc accaccagaa gtcgatggga ccgacctggc gcaacacaaa
     2281 catcgggctg aacatcagga cggccaggca ggagatgaga atggcaggtc ggatattaat
     2341 gatcttatga gatggcatcc cgaatcccta ttttgttgaa gccaataaac ttatttatag
     2401 ttagttataa acaatacgac gttgatttga gatgccatct cattttgccc agtcaataac
     2461 agcctggagg atcagggcca gggtacagag cgatccgaaa agcaggttca attgcgccga
     2521 tgcgccgtcg agggcaatga aatcaagcgg ctgggccggt gttttccggt tcagcgcttt
     2581 tttccagacc ctgaaagcca gcggaagcgc ggccagcgtg atcaggaatg taaacggcat
     2641 ggcggtcatg ttcgggaaca ggaaaaacgg gaccaggatc aggcccacta tgatgataaa
     2701 cggtccgtaa atcaaaacgc cataataccg taatgaattc ttatctccca gtaatgaggc
     2761 gatggtaaat atcttgatct ctttgtccga ctggatatcc ctccagttat tggcatgcag
     2821 gatggcaatg accagcatcg acatcgggat ggcccagatg acaggaatcc atgatagagc
     2881 gccggattgc acataatagg cgcccagcga gcccaggatg ccgaaattga ggaatacggc
     2941 caggtcgcca agcccgttgt atttcatgtt gaccatggaa tgggaggtgt aaaacgtacc
     3001 cactaaaagg ccgagaatac cgatagccag caaccagggt ccgctcaccc aggtcaggta
     3061 cagcccgatg atcgtcccga aaaccagcat ggagacagaa ccgatccatg cttcgcgtat
     3121 ggaaataatt cccctgacaa ctccgccgct ggtcgggccg gggaccttat cgagcccttt
     3181 ctcaaaatca aatatatcgc tcagaatatt tgcccccgag tgaaggatga ccatcgctat
     3241 aaaagctaaa ataaacagca gcggcttgaa ttcgtgtccg ccataaacat aggccagcac
     3301 cgtcccaaaa ataacgggca tggtggaagc ggggaatgaa aacggcctgg ccgatatcca
     3361 ccacttctta aagaatgaga gttttgcctg atcttccatt aaattattat ccggttgata
     3421 ctcaaacaat actgagtaag atttgttcaa ggctgcaaag ataatgaaat ttgattttcg
     3481 gaagaatttc cggatcaggg cattaggagg actttccggg tttctgatga tttggcaccg
     3541 atcaaatgga cgtaatagag tccgggggag tatttgaaat gatcgaaaat aacatgattg
     3601 taatgaccgg tttccttctg tgaaatcatc ttacccaaac cgttataaac tctaatcgcc
     3661 tcaaaatcac cgggtttttt ccaggaaact ttgaaagccc ccgaggcagg gttcgggctg
     3721 atcatcaccg ttgtttctcc ttcatcctca atacctgagc aatatgaaaa atcgaaagag
     3781 accaggatcg tgtcggtaaa tgtgcatcca tccactgtgg tagtcgttag tgaataatct
     3841 tgcaggctga agccaattcc cgtgacggtc acaaagattt cgggggtagt cgcgccgttc
     3901 gaccagagaa aattccagga aggatcacgg ggaacaggtt gcagcaacac cgaatcattc
     3961 ggacaggcaa acacatggtc agagccgtca gcccatccga attcagccga cggaaagacg
     4021 accagggtga aatcataccc attgacattg tacccatcgt cgaccgaaac atgaaagacc
     4081 gtcgtatctt ccggcgatac cgtgatctgg gccagcgaag gatatgaatt gcccaggtta
     4141 tcgctccagg agtagatata gtttccctgg ttgccgccgc tggcatgtgc ggtgagaacg
     4201 gcctcttcgc cccggcagat ctttggcttg tcgctggtga tgacgaccgc cagcgggccg
     4261 ccgtcgatgc tgatggtaat gggatcggga ttgctgacac agcctgtttc aaggtcggtg
     4321 accgtcaggg taaaaacggt agcgatgatc aggttctggg tctgcgtaac gggtatatcc
     4381 gggtctgaga ccaaagcggc cggttcccag tggtatgcat agctgccact acctcctgat
     4441 gcagtggccg atagcgtggt attggtgccg tatgggatca actggtcagg gccggcatcc
     4501 ggctggggat tcggatgaac ggtaattgtg accgatgcag tcatttcact cagcccgtcg
     4561 ctaacggaaa cggaataggt tgtcgtagta gccggttgaa cggtgacttc cgggagatcg
     4621 gatgtaaagc cgggtgggga ggatgaccag gaataggaat agttttcagt acctccgtag
     4681 ggtatagcgg ttaaagtcgt ctgctggccg atacatacgg cttccggatc ggctgcgatg
     4741 tttaccccca gtggaccgcc ggttacctgt atgatgaccg tgtcgtatcc ggtgcaattg
     4801 gcagcctggt cggtaacggc caggataaat ccggtggtgg caaatagtgc gtttgtctgg
     4861 ggattcagcg agcccggatc agtcagcaac gccgccggct cccaactcca ggcataaggg
     4921 ccggtgccgc ccgaatagct gccatgcaac gtcgtggaag atccgtaagg gatgatgggc
     4981 gcatcggcgg tggctgccac caccgggctt tcgccgacca aaacaccggc agtggccgta
     5041 tcggaggcac atccatcacc ttccacccag aggctgatcg tattattccc aatagagtta
     5101 tgcacgattt cgtaaggacc aggtccgtta ccggaaagta cggaggcatc tccaaaatcc
     5161 cagttaaaaa tcgcggtatc cggggcattt ccctggtagg tcacggtgac aatttcgcgg
     5221 gtacaggcag cctgaggtgc aacaaaagtg gaggtaaact ggggaaggac ctccacgtag
     5281 accgtatcga agtaaacaag aacagtggca aacgagatgt cgtcgagggc gaaatcgtta
     5341 ccgctcagga tcgtattctg gttgacaata ctgatagtgg cctgggtatt gtcgccggct
     5401 tcccagatgt ggaagaactg gtaccagtcg caggtgtaag gataagcctg gaaaggttct
     5461 cccatgagtt ccccgttgat gctgaactgg aggatggccg ggttggtagc gtggaggctg
     5521 gtgatccagg tcgagaattc atatttgctg ccgggctgga tgttgttcag ggagagatac
     5581 cagaccttga cattggctac cccggcgccg ttgaccgcca taaatttccc gctgccgctg
     5641 gtgtggtctt cgttgcagta gaaattcggg tgcaccgtat gggcgtcgtt caccacggca
     5701 taggtccctt ctgccacaat gctgacctgg ttgttgacgt attcgctgtt aaaacccatc
     5761 acgccgagtt caaacgagcc gttgaggatg aggttaccgg tagcaatatt cgactggacg
     5821 acatacatgg tgcttacctg gggcgaaacg accgggtttt gttgctgtgg gatcaggagc
     5881 gaagggtcgg taggatagga gctccagaag tagctgacgc catcgccgtt ggcaaagagc
     5941 tgggctgatc cgccctggca gatggtggta tcgccccagg cttcgagctg gctgtgggcg
     6001 ggatgggtga aaagggtgag gaagataaga agataggcga tatggagtga ttttgatttc
     6061 aagggtacgg ttgatatagg aaggtaaaat tactatttat taaggagact ccatctcagt
     6121 taagtgagac tccatcttaa agtgaaattt ttcttcgaga tgga
//
LOCUS       DAOPNH010000208         6142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14724_length_6142_cov_6.995564, whole genome shotgun sequence.
ACCESSION   DAOPNH010000208 DAOPNH010000000
VERSION     DAOPNH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_6142_cov_6.995564"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKZ74_10680"
     CDS             complement(<1..450)
                     /locus_tag="PKZ74_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPM93265.1"
                     /translation="MTTPPYLKKGDKIALVSPARKVTPEEISPAKELFESWGLEVVEG
                     ENLYAAYNQFAGSDAQRTADMQRALDDDSIRAIVCSRGGYGTVRIIDRLDFNRFASHP
                     KWIVGFSDITVLHSHIHRHLNIETLHAIMPVNIRERCESDRSLSSMRK"
     gene            647..2710
                     /gene="metG"
                     /locus_tag="PKZ74_10685"
     CDS             647..2710
                     /gene="metG"
                     /locus_tag="PKZ74_10685"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPM93266.1"
                     /translation="MENKNWKRTTVTSALPYANGPIHIGHLAGVYVPADIYVRYLRSK
                     GEEVLFIGGSDEHGVPITIKARKEGITPQQVVDRYHEIIKDSFEKFGISFDIYSRTSH
                     PIHHETASEFFTTLYNKGEFIEQTSLQYFDEEQQHFLADRYITGTCPRCGNPKAYGDQ
                     CESCGTSLSPDELINPVSALSGNAPVKKETKHWYLPLDKYQDWLEKWIVEGHKDDWKP
                     NVYGQCKSWLDHGLQPRAVTRDLDWGVKVPLPGTDGKVLYVWFDAPIGYISATREWGQ
                     IHGKDWKPWWKDQETRLIHFIGKDNIVFHCIIFPSMLKEEGSFILPDNVPAFEFMNLE
                     NDKISTSRNWAVWLHEYLEEFPGRQDELRYVLTTNAPETKDNDFTWKDFQTRNNSELL
                     SIFGNFVNRTLVLTYKYFEGKIPAKGELSSADREAIDEIRTFPGRIGGSLEQFRFREA
                     LSEMMNLARLGNKYLTDAEPWKVYKTDPERVKTILNISLEICANLAILCEPFLPFTAE
                     RLSRMMNLPQRKWAGAGNPELLEAGHQLDQPEYLFTKIEDDVIDAQVQKLLATKQQNA
                     AVAEPKINLKPMKETITYDDFTKLDIRVCTVEAAEKVQGADKLLKLTVNTGLDTRTIV
                     SGIALYYKPEELIGKQFCFIINLAPRKLRGIDSNGMILSAENPDGTLSLIRPGDLLEN
                     GSEVK"
     gene            2817..2888
                     /locus_tag="PKZ74_10690"
     tRNA            2817..2888
                     /locus_tag="PKZ74_10690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Arg,seq:cct)
     gene            2915..3403
                     /locus_tag="PKZ74_10695"
     CDS             2915..3403
                     /locus_tag="PKZ74_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM93267.1"
                     /translation="MGNGKRITPFFSLPAARRGKMKQETSRPIPFKAEITRILDYWHN
                     AASRADMPAYFGMIDEEGIYIGTDATENWTKQAFYDWSKPYFDKGKAWSFHAEERKIY
                     LLEDLKMAWFDEKLSSASGPLRGSGVLINRNGQWKILHYVLSLPVPNERFKEVIELIN
                     EE"
     gene            complement(3432..4853)
                     /locus_tag="PKZ74_10700"
     CDS             complement(3432..4853)
                     /locus_tag="PKZ74_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPM93268.1"
                     /translation="MPFRLAFISEKHRKYKTDQWLVFTPRHKPDESLYAHLVFAMKYE
                     GIDLLFFKKLFEKLEPGEISAWVLHEPQSQYGRRIWFLYEWLMQKPLEVPDLNNGNYV
                     PLLNDELQYASPTGINSKRHRIKNNLTGNVNFCPLIFKTDKIERYIAENLSAKTERVI
                     RDVHKDLLLRTSAFLLLKDSKASFTIEGEQPAHTRALRWGKAIGQAGTNPLSIDELLR
                     LQQIVIDNSRFIHMGLRNEGGFIGEHDRTTGEPIPDHISARWQDLEILLEGIIEADRL
                     LETDGFDPVLTATMIAFGFVFIHPFEDGNGRIHRYLIHHILSSTRYTPQGIIFPVSAA
                     ILEKIEDYKKVLVSFSRPLLDFIEWRKTSFNNIEVLNETIDYYRYFDATRQAEFLFEC
                     VDYTINKIIPEEVSYLRSYDEMKLWLDHRFQMPDKTIALLIHFLNQNNGKLSKRAREK
                     EFASLTDIEINEIEQQFGSCFKS"
     gene            complement(5386..5979)
                     /locus_tag="PKZ74_10705"
     CDS             complement(5386..5979)
                     /locus_tag="PKZ74_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPM93269.1"
                     /translation="MELNPNRRRLVDLSGVCIQVWMAENLKVTRYRNGDAIQFVMGNQ
                     EWDELHTGAYCDMHNEGLNASSFGHLYNWYAVNDGRGICPPGWHVPRDDEWDVLIGFL
                     GGEDVAGGKLKSTDSLLWESPNTSAADLVGFAGLPGGTRWEKGLFLYFGFYGLWWSST
                     AEDADFAYYRNLIYDKPACFRNHFMKNHGCSVRCIKD"
BASE COUNT         1653 a         1528 c         1406 g         1555 t
ORIGIN      
        1 ttttctcatg ctgctgagcg agcggtcgct ttcgcacctt tcacggatat tcacgggcat
       61 gatagcgtgc agcgtttcaa tgttaagatg gcggtggata tgcgagtgga gcacggtgat
      121 atcgctgaat cccacgatcc acttgggatg cgaggcaaac cggttaaaat cgagccggtc
      181 gattatgcgg accgttccgt aacccccgcg ggagcaaacg atcgcgcgga tcgaatcgtc
      241 gtcgagtgcg cgttgcatat cggccgtgcg ctgggcatcg gagccggcaa actggttgta
      301 ggcagcgtat agattttcac cttccaccac ctccagtccc cacgactcaa atagttcctt
      361 cgccggagag atctcttccg gtgtcacttt ccgggccggg gagacgaggg cgatcttatc
      421 gccttttttg aggtacggag gtgttgtcat tttatatttt tttcatccaa agaaaagcaa
      481 agatcggtat ttatgttcac ttttcattaa ccccataggc acatcgggca catagataca
      541 catagaaaat ctattgtgcc ttctatgtgt cctatgtgcc tatgtggtga aaaaaagctc
      601 aatatacccg ataaatccct aattttgccg caaacatttg atcttcatgg aaaacaaaaa
      661 ctggaaacgc actaccgtca cctctgcatt accctatgcc aacggcccca tccatatcgg
      721 acacctggca ggcgtttacg tacccgctga tatatacgtc cgctacctcc gctccaaagg
      781 cgaagaggtg ctctttatcg gcggctccga cgagcatggc gtgcccatca cgatcaaagc
      841 gcgcaaagaa ggcatcaccc cgcagcaggt cgtcgaccgg taccatgaga tcatcaaaga
      901 ttcgtttgaa aaattcggca tctcgttcga tatctattcc cgtacttccc atcccatcca
      961 ccacgaaacg gcctctgaat tcttcacgac gctttacaac aaaggcgaat ttatcgaaca
     1021 gacctcgctg cagtattttg atgaagaaca gcagcatttt ctcgccgacc gctacatcac
     1081 cggcacctgc ccgcgctgtg gcaacccgaa agcttatggc gaccagtgtg aaagctgcgg
     1141 cacctccctt tcgcccgacg aactcatcaa ccccgtctca gccctcagcg gcaatgcacc
     1201 ggtcaagaag gagacgaagc actggtacct gccgctcgac aaataccagg actggctgga
     1261 gaaatggatc gtggaagggc acaaggacga ctggaagccc aacgtttacg ggcagtgcaa
     1321 gtcgtggctc gaccatggcc tgcagccccg cgccgtcacc cgcgacctcg actggggtgt
     1381 gaaggtaccg ctgcccggca ccgacggcaa ggtgctgtac gtctggttcg acgcgccgat
     1441 cgggtacatc agcgctaccc gcgaatgggg gcagattcac ggaaaagatt ggaaaccatg
     1501 gtggaaagac caggaaacaa ggctgatcca ctttatcggt aaagacaaca tcgtttttca
     1561 ctgcatcatt ttcccctcga tgctgaagga agaaggcagt tttatcctcc ccgacaacgt
     1621 ccccgccttt gagttcatga acctggagaa cgacaagata tccacctcca ggaactgggc
     1681 cgtatggctg catgagtatc tcgaggaatt cccgggcagg caggatgagc tacgttatgt
     1741 cctgacgacc aatgctccgg aaaccaagga caacgatttc acatggaagg atttccagac
     1801 ccgcaacaac agcgagttgc tgtcgatctt cggcaatttt gtgaatcgta cccttgtcct
     1861 tacctataag tattttgaag gaaagatacc ggcgaaaggc gaacttagtt cggccgaccg
     1921 tgaagccatc gatgagatca ggactttccc cggcaggata ggagggagcc tggagcagtt
     1981 ccggttccgc gaggcgctga gcgaaatgat gaacctggct cgtttaggaa acaaatacct
     2041 gaccgacgcg gagccgtgga aggtttataa aaccgacccg gagcgtgtga aaacaatttt
     2101 gaacatttcg ctggagatct gcgccaacct ggccatcctc tgcgagccgt tcctgccctt
     2161 caccgccgaa cgcctcagcc gcatgatgaa ccttccgcag cggaaatggg ccggcgcagg
     2221 caatcccgag ctgcttgaag ccggccacca gctggatcag cccgagtatc tctttaccaa
     2281 gatcgaagac gatgtgatag acgcccaggt gcagaaactg ctggccacaa agcaacagaa
     2341 tgctgcggtc gctgagccga agatcaacct gaagcccatg aaagagacca taacctacga
     2401 tgattttaca aagctcgaca tccgtgtctg cacggtagaa gctgccgaaa aggtgcaggg
     2461 ggccgacaag ctgctgaaac tgaccgtcaa cacagggctc gacacccgga ccattgtcag
     2521 cggcatcgcg ctctactaca aacccgagga acttatcggc aaacagttct gcttcatcat
     2581 taacctggcg ccgaggaagc tgcgcggcat cgactccaac ggcatgatcc tctcggccga
     2641 gaatcccgac ggcaccctca gcctgatccg cccgggcgat ttgctggaga acggaagcga
     2701 agtaaaataa tgttgaatgt tggatgttga aggttgaatg agggaattct ttaaaaattt
     2761 actaattttg cacccgaatt caacatttaa ccttcaacat tcaacatttc ctgcatggcc
     2821 ctatagttca acggatagaa cggaagtttc ctaaacttta aatccaggtt cgattcctgg
     2881 tggggctaca caaattcaga atgttcatgc cacaatggga aatgggaaaa ggatcacgcc
     2941 tttcttctct cttcctgctg ccagaagggg caaaatgaag caggaaacca gtcggccgat
     3001 tccctttaaa gcagaaatca cccggattct tgactattgg cacaatgctg cttcccgggc
     3061 agatatgccg gcgtatttcg gcatgatcga tgaggaagga atctatatcg gcaccgatgc
     3121 aacggaaaac tggactaaac aggcgtttta cgactggtca aaaccgtatt tcgacaaagg
     3181 aaaagcctgg tcgttccacg ccgaagaacg caaaatttat cttttggaag acctgaaaat
     3241 ggcctggttt gatgaaaagc tcagttctgc ctccggcccg ttaagaggtt ctggtgtgtt
     3301 gatcaacaga aacggtcagt ggaagatttt gcattatgta ctttccttac ccgttccgaa
     3361 tgagaggttc aaggaggtga ttgaactgat aaatgaagaa tgattttcgg gaaccatata
     3421 attatccgtg ttcaggactt aaaacaggag ccgaattgtt gttcaatttc attaatttct
     3481 atatcggtca gtgaagcaaa ttctttttcc ctggctcttt tagaaagttt gccattgttt
     3541 tgatttaaaa aatgaattaa aagtgcaatc gttttatcag gcatttgaaa acgatggtcc
     3601 agccataatt tcatttcatc atagcttcgc agatagctca cttcttcggg aataatctta
     3661 tttatagtgt aatcgacaca ttcaaaaagg aactcagcct gcctggtcgc atcaaaatac
     3721 cggtagtaat cgatggtttc attcagcact tcgatattgt tgaaggatgt tttccgccat
     3781 tctataaaat caagcaaagg ccgggaaaaa gataccagaa cttttttata atcttcgatc
     3841 ttttccagta tggcggccga gaccgggaaa ataatacctt gtggagtgta tcttgttgaa
     3901 gataagatat gatgaattag gtagcggtga attcttccgt tgccatcttc aaagggatgg
     3961 atgaaaacga aaccaaaagc aatcattgtg gctgtaagaa ccgggtcgaa gccatccgtt
     4021 tccagcaaac ggtccgcttc aattattccc tcaagcagga tttcaaggtc ctgccacctg
     4081 gctgaaatat gatccgggat gggttcgccg gtcgttctgt cgtgttcccc gataaatccg
     4141 ccttcgttgc gtaaacccat gtggataaac cgggaattat cgattacaat ttgctggaga
     4201 cgaagcagtt catctatact taacgggttg gtcccggcct gtccgatggc tttgccccag
     4261 cgcagcgccc gggtatgagc tggttgctca ccttcgatag taaaagacgc tttagagtct
     4321 ttaagaagca gaaatgccga cgtgcgcaac aacagatcct tgtgtacatc ccggatcacc
     4381 ctttccgttt tcgcagataa attttcagca atataccgtt caattttatc cgtcttaaat
     4441 ataagcgggc aaaagttgac atttcctgtt aaattgtttt tgatccggtg acgttttgaa
     4501 ttgatacctg taggactggc atactgcagt tcatcattca gcaaaggcac atagtttccg
     4561 ttatttaaat cagggacctc cagcggtttt tgcatcagcc attcataaag aaaccagatc
     4621 ctgcggccat attgactttg cggctcatgc aagacccagg ccgaaatttc gcccggttcg
     4681 agcttttcaa atagcttttt gaaaaacaac agatcgatgc cttcatattt cattgcaaaa
     4741 acgaggtgcg cgtataaaga ctcatccggc ttgtgccttg gcgtgaaaac cagccattga
     4801 tcggttttat attttctatg cttttcactg atgaatgcta aacgaaaagg cataggaacg
     4861 ggaagttcac aggcttgtat aatggctcca tatcccacta aatagccccg ctcaggagct
     4921 tttctaccgt gaaaaacact tatttgacgt gaaatatgct tattttgctg catattttat
     4981 gaaatatact tttagcaaaa ataaatgata aacacttaca attcatgaat attgattaaa
     5041 gattatctga ttctgtgaaa aataatttca tgccatttac agcggcattg tcattgttgc
     5101 tctgactata atataattcc ccctgttctt aggacagaat ttctttaaaa ttaagtttat
     5161 tattgattta gtggagtgcc tgggtaattt ttgacaatga aaatctacca ttatgctacc
     5221 aatcttccga aaggcttgat atcattgggc tgttcatttc ctggcggggc tacgaaaaaa
     5281 gcaatataag ccggtgtaga tcaacaaact gcaccggttt attttttgac gggggacggt
     5341 agaggggacg tcacacttat acaatcggtc ttagccccaa agcgctcaat ccttgatgca
     5401 cctcacacta catccatggt ttttcatgaa atgatttctg aaacaggcag gtttatcgta
     5461 aatcaggttc ctgtagtagg caaagtccgc atcctctgcc gtcgaagacc accataaacc
     5521 ataaaaacca aagtacagga atagtccttt ttcccatctt gtcccgccgg gcaaacccgc
     5581 aaatccaacc aggtcggcgg cgcttgtatt cggggattcc cagagcaggg aatcggtgct
     5641 tttcagcttc ccccctgcaa catcttcacc ccctaaaaac ccgatcagga catcccattc
     5701 atcatcacgg ggaacatgcc agcccggtgg acaaatgcct cttccatcat ttactgcata
     5761 ccaattgtac aaatgaccaa aagaagacgc gttcaatcct tcattgtgca tatcgcaata
     5821 cgccccggta tgcaactcat cccattcctg gtttcccatt acaaactgaa tggcgtcacc
     5881 atttcgatat cttgtaacct taagattttc agccatccaa acctggatgc atacaccaga
     5941 caaatcgacc agtctgcgcc ggtttggatt taattccata gttgcgcttc atttcctgac
     6001 tttagatgac catattttgc aggcaaaggc tggtaatttc atcgtctctg tccaatgatg
     6061 aacctgatga cggctgtttt ggccgtgccg ccatctcgcc ttcccagctt ttaaaaagtg
     6121 taaactgaaa atgtcaaaaa gt
//
LOCUS       DAOPNH010000209         6132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14758_length_6132_cov_5.369426, whole genome shotgun sequence.
ACCESSION   DAOPNH010000209 DAOPNH010000000
VERSION     DAOPNH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14758_length_6132_cov_5.369426"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKZ74_10710"
     CDS             complement(<1..354)
                     /locus_tag="PKZ74_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPM93270.1"
                     /translation="MKTKERESLFSAKNRKLIINPLNADNPITVQVLGICSALAVTAK
                     LKPAFVMALSVVVVTAFANLVISLIRNGIPPRIRIIVQLTVVATLVILVDQLLKAYVY
                     DVSRQLSVFVGLIITN"
     gene            complement(358..1101)
                     /gene="nqrC"
                     /locus_tag="PKZ74_10715"
     CDS             complement(358..1101)
                     /gene="nqrC"
                     /locus_tag="PKZ74_10715"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPM93271.1"
                     /translation="MYSNSYVFRYAAIMVIVVAAALASVAMLLKPAQDRNVTIEKMRN
                     ILAAANIEATPENAIELFDKYVIEEEALAPDGNVVAVFRGQKLEKGNIRPFEINLKEE
                     LYKHTKGQDFIIPLYIAEVNGKRLYIFPMLGRGLWGPVYGNLALEEDFNTVAGSMFGH
                     DKETPGLGAEIELPQFESQFIGKKIFDDNGNFTSIQVLKGASEVLSPEKLIHGVDAIS
                     GGTITSNGVSDMIKSNLANYIEYIKKQKI"
     gene            complement(1133..2278)
                     /locus_tag="PKZ74_10720"
     CDS             complement(1133..2278)
                     /locus_tag="PKZ74_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPM93272.1"
                     /translation="MKSLRNFLDKVKPQFEKGGKFAKFQSTFDAFETFLFVPNKVTRS
                     GSHIRDAIDMKRTMSVVILALLPALLFGIYNVGYQHFLSHGQEAGFWQMFGFGLVKVL
                     PIIVVSYVVGLGIEFIFAQSRGHEVNEGYLVSGMLIPLIVPPDIPLWMLAVSVAFAVI
                     FGKEVFGGTGMNVLNPALTARAFLFFAYPQDMSGDKVWIAEKADAFSGATPLGHALVG
                     DFDKIQAPIDMFLGFIPGSIGETSVLAILIGAVILIATGIGSWKIMLATVIGALGLGY
                     FLNLVSFNDYTALPAHYHLLMGGFAFGAVFMATDPVSAAQTNAGKWIYGILIGVFVVL
                     IRVFNPAYPEGMMLAILLMNVFAPLIDYYVVEANIRRRLKRMKPAKA"
     gene            complement(2410..3759)
                     /locus_tag="PKZ74_10725"
     CDS             complement(2410..3759)
                     /locus_tag="PKZ74_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPM93273.1"
                     /translation="MSNVIRISKGLTINLIGEAEKTISEIVPKTCAIKPTDFIGVFPK
                     LLVQEGDAVKAGTPLFIDKYRDKIVFTSPVSGTVKEIRRGEKRVLLEVVIENDGKDTG
                     IDFGKGDPEKMTREEITGKLLASGLWPFLRQRPYSIIAEPASKPKAIFISAFDTAPLA
                     PDYDLIVHKNGDVFQTGINAIAKLTDGAVHLNLNASTTSSGVFTNCKNVQINRFSGPH
                     PAGNVGTQIARLDPINKGEIIWYLRPQDVLHIGRLFAAGKIDATHLVAVTGSEIKRPH
                     YIKTKLGAAVEDIIKDNLSGEHVRYISGNPLTGKKVERYSHTGFYEAQLTVLPEGDHH
                     EFLGWAMPGFGKFSFSRSFFSWMKPKARYRLDTNYHGGERAYVMTGQFEKVFGWDIYP
                     LQLIKAILVEDIDMMEKLGIYEVDEEDFALCEFIDTSKTDIQEIVRKGLDLMRKEMS"
     gene            3850..5151
                     /locus_tag="PKZ74_10730"
     CDS             3850..5151
                     /locus_tag="PKZ74_10730"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPM93274.1"
                     /translation="MKKAVILFISALLSFNLLPFNLVSQNEPYYDAGYIRNDNAVYRE
                     TVRTVLLYREGFELAPPLIEFGSGEKLVLSFDDLDPQYKDYRYTVVHCDADWNTSDLR
                     QMEYLEGYFEDFIEDYAYSFNTTIPYVNYTLVFPTDDLRLKKPGNYILKVFLDSDKDE
                     NIILTHRFMVYEPKVAVEGKTNNSIDLDLRYTHQQLEFRLLAGDYNMSDVHRNLHVVI
                     MQNGRWDNAIRNIQPRNYSGNIYDFTLINGLVFPAGNEFRYFDMKTLRYNTDRMQLLQ
                     YDTDGYHVYLMTDLPRASGSYISDEDINGRRLIAANETRDPYSEGDYAWVHFLLPYWP
                     PLASGNLYIAGAFSDWTYNRENLCIYNFDLKAYEAKILLKQGYYNYAYAFVANNTAVG
                     DLTWVEGSFWETKNEYSILVYYREPGDTYDKLVGVGYVNRE"
     gene            complement(5202..>6132)
                     /locus_tag="PKZ74_10735"
     CDS             complement(5202..>6132)
                     /locus_tag="PKZ74_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93275.1"
                     /translation="NNCWVDHSDPSLRLIPLGYYDWDPVWCPGESYLKSDDVAETMFY
                     EAKAEIEAENYQAADSGFKQIIAGFPENRFALASLKELFALNPVLYSNYATLKAYCDN
                     LSENPGDSLLGKTAEWISIHCNIRDQHYQQAINSLDSIIFNPGTQADSVFALIDLAYV
                     FMQNNSDTAMKSVLVTKHPELVPESKNQFNRQRKEWIDILLQPKGGSSLGANEMIDPP
                     GEFPTCRITSIRPNPSKGDFTVDMDVRKPGEIRLLIVSPTGAIIYEFDLFAENPGLYR
                     KAISEPSLQPGVYYLLVSVAQEKPDAKKLVIIN"
BASE COUNT         1525 a         1499 c         1498 g         1610 t
ORIGIN      
        1 gttggtgata atgagtccga caaaaaccga taattgccgg ctgacatcgt aaacataagc
       61 tttcaggagt tggtcgacca ggataacaag tgtggcgact accgtcagct ggacgatgat
      121 ccggatccgt ggcggtatcc cgttccgaat cagggatatg accaggttgg caaaggctgt
      181 gacaaccact accgacagcg ccataacgaa tgccggtttc agttttgccg taacagccag
      241 cgctgaacag atacccagaa cctgtaccgt gatagggtta tccgcattca gcggattaat
      301 gatcagcttc cggttctttg cagagaaaag ggattctcgt tcctttgtct tcatcggtta
      361 tattttttgc tttttaatat attcaatata attagccagg ttggatttga tcatatccga
      421 aaccccgttg ctggtgatcg tgccaccgga gatcgcatcc acgccgtgaa ttaacttttc
      481 aggcgaaagg acttcggaag cgcctttcag tacctggata gaggtgaagt tgccattgtc
      541 atcgaaaatt ttcttgccga taaactgcga ttcgaattgt ggcaattcga tctcagcccc
      601 cagtccgggg gtttctttat catgtccgaa catcgagccg gcaaccgtat tgaaatcctc
      661 ttccagcgcc aggtttccat ataccggccc ccacagcccg cggccaagca tcgggaaaat
      721 atacaaccgc ttcccgttca cttcggcaat atagagtgga ataataaaat cctgcccttt
      781 ggtatgtttg tagagttctt ctttcagatt gatctcaaac gggcggatat ttcccttctc
      841 gagtttctgt cctctgaaga cggccaccac attgccatcg ggagccagcg cttcctcttc
      901 gataacatac ttatcgaaca gttcgatcgc attttcggga gtggcctcga tattggccgc
      961 agccaggata tttcgcatct tttcgatggt cacgttgcgg tcctgtgccg gcttcagcag
     1021 catggccacc gaagcgagcg ccgcagccac cacgatcacc atgatggcgg cataccggaa
     1081 cacatatgaa ttactgtaca tatcgttgtt gtttattccg tttcaaatta aatcacgctt
     1141 tagccggttt catccgtttc aacctccttc ggatattggc ttccaccaca tagtagtcga
     1201 tcagcggggc aaagacattc ataaggagga tggccagcat catcccttcg gggtaggccg
     1261 ggttgaacac acggatcagc acgacaaata cccctatgag aatgccgtaa atccatttcc
     1321 cggcattggt ctgcgctgca ctgaccggat cggtggccat aaacaccgcg ccaaaggcaa
     1381 atccgcccat gagcagatga taatgggccg ggagtgccgt atagtcgtta aaggagacaa
     1441 ggttaaggaa gtatcccagg ccaagtgcgc cgataaccgt cgccagcatg atcttccagc
     1501 tgccgattcc ggtagcgata aggataaccg ctccgatgag tatggccagg actgaagtct
     1561 cgccgatcga tccggggatg aatcccagga acatgtcgat gggggcttgt atcttgtcaa
     1621 aatcaccgac cagggcgtgg cctagcggag tggccccgga aaaagcgtct gccttttcgg
     1681 caatccagac tttgtcgccc gacatatcct gcgggtaggc aaagaagagg aatgcgcggg
     1741 ctgtcagggc cgggttcagc acgttcatgc cggttcctcc gaacacctcc ttaccgaaaa
     1801 ttactgcaaa agcgacggaa acagccagca tccacaatgg gatatcgggc ggtacgataa
     1861 ggggtatcag catgcccgat acgaggtaac cttcgttgac ctcgtgtccg cgcgactggg
     1921 cgaagatgaa ctcaatgccc agcccgacca cataggaaac gacgatgatc ggaagtacct
     1981 taacgagccc aaagccgaac atttgccaga aaccggcttc ctgtccgtgg gagagaaaat
     2041 gctggtatcc gacattataa ataccgaaaa gcaatgccgg caacagtgcc aggatcacaa
     2101 cgctcatggt gcgcttcata tcgatggcat cgcggatgtg gcttccgctg cgggttacct
     2161 tgttcggcac gaaaagaaag gtttcgaaag catcgaatgt cgattgaaac tttgcaaact
     2221 tccccccttt ttcgaactga ggcttaacct tatccaggaa attcctgagt gacttcatca
     2281 gaaaaaaata ttttaattct taatttttaa tttttaattc ttaatggctg atatttatct
     2341 atttatacct ttaaagaact cactttgaac tttgatccgc cagctggcgg atgaactttg
     2401 aactaatatt tagctcattt ctttccgcat caggtccagg cctttacgga ctatctcctg
     2461 gatatccgtt tttgatgtgt cgataaattc gcaaagggca aagtcttctt catccacttc
     2521 ataaataccg agtttctcca tcatatcgat atcttcgaca agtatggctt tgatcagctg
     2581 gagcgggtag atatcccatc cgaagacttt ctcgaactga ccggtcatga cgtatgccct
     2641 ttccccgcca tgataattgg tatcaagcct gtacctggct tttggtttca tccaggaaaa
     2701 gaaagacctg gaaaagctga attttccaaa tccgggcatg gcccagccga ggaattcatg
     2761 atggtcgcct tccggcagca cagtcagttg ggcctcataa aatccggtat ggctgtacct
     2821 ctccactttt ttgcctgtga gcgggtttcc gctgatatac cggacatgct ctccggaaag
     2881 gttatccttt ataatatctt caaccgcggc ccctaacttc gttttgatgt aatggggccg
     2941 tttgatttcc gatccggtaa ctgcgaccaa atgagtcgca tcaattttcc cggcagcaaa
     3001 taaccgcccg atatgcagca catcctgcgg cctgagatac cagataattt cacctttatt
     3061 gattggatcg agacgggcga tctgggtgcc tacattaccg gccgggtgtg gcccggagaa
     3121 acggttgatc tgcacattct tacagttcgt aaatacgccg gaagaggttg tactggcatt
     3181 caggttgaga tgtacggccc catcggtcag cttagctata gcattgatac cggtctggaa
     3241 aacatcaccg tttttatgga cgataagatc gtaatccggg gccaggggtg ccgtgtcgaa
     3301 ggcggaaatg aagatggctt tcggcttcga ggcaggttcg gcaatgattg aataaggccg
     3361 ttgcctgagg aaaggccata agccgcttgc caggagctta ccggtgatct cttcacgggt
     3421 cattttctcc ggatcccctt tcccgaaatc gatgccggtg tcctttccgt cgttctcgat
     3481 gacaacctcc agcaggacgc gcttttcgcc gcgcctgatc tctttcaccg ttccgctgac
     3541 gggtgaggtg aagacgatct tgtccctgta tttgtcgatg aataaaggcg ttccggcttt
     3601 cacggcatcc ccttcctgga ccaggagttt gggaaacaca ccgataaaat cggtcggttt
     3661 gatggcacag gttttgggga cgatctcgga aatggttttt tcggcttctc ctatcaggtt
     3721 aatggtgagg cctttgctaa tcctgattac atttgacata ttaaaaatag tctatatttt
     3781 cggcagtgca agtgtacaaa attttgcgaa atcttgcgac ctttgttgta aattttaccc
     3841 gtgaaagata tgaaaaaagc cgtaatcctt ttcatttccg cgctactttc ttttaacctg
     3901 ctgccattca atctggtatc acagaacgaa ccctattacg atgccggata tatccggaat
     3961 gacaatgcgg tttaccggga gacggtacgg actgtgctgc tttacaggga aggatttgag
     4021 ctggctccgc ccctcattga atttggctcc ggagagaaac tggtgctgtc gttcgacgac
     4081 ctggatccgc aatacaagga ttaccgttat accgttgtcc attgcgatgc cgactggaac
     4141 acctccgatc tccgtcagat ggaatacctc gagggttatt ttgaagattt tatcgaagat
     4201 tatgcttatt cgttcaatac gaccattccc tatgtaaatt acacccttgt attcccgacc
     4261 gatgatctgc gcttgaaaaa accgggcaat tatatcctga aagttttcct cgattcggat
     4321 aaagatgaga atatcatcct gacccaccgg ttcatggtgt atgaaccgaa agttgcagtt
     4381 gaggggaaga ccaacaattc gatcgacctg gatttgcgtt acacccacca gcaactggag
     4441 ttcaggctcc tggccggcga ttacaacatg tcggatgtcc accggaacct gcatgtggtc
     4501 atcatgcaga atggccgctg ggacaatgcg atccgaaata tccagcccag gaattactcg
     4561 ggaaatatat atgatttcac cctgatcaac ggcctcgtct ttccggccgg caatgaattc
     4621 cggtacttcg acatgaagac gttgcggtac aataccgacc ggatgcagtt gctgcagtat
     4681 gatacggatg gttaccatgt ttatctgatg actgacctcc cccgggccag cggcagttac
     4741 atttctgacg aggatattaa cggccggagg ctgatagcgg ccaacgaaac ccgcgatcct
     4801 tacagtgaag gcgattacgc ctgggtgcat ttcctactgc cttactggcc gccgctggcc
     4861 agcgggaatc tctacattgc cggcgccttc tccgactgga cctataaccg ggaaaacctg
     4921 tgcatctaca actttgatct caaggcctat gaggctaaaa tactgctgaa gcagggctat
     4981 tacaactatg cttatgcctt cgtggccaac aatacggctg tgggcgattt aacatgggtg
     5041 gaagggagtt tctgggagac gaagaatgaa tattcgatcc tggtctatta ccgcgagccg
     5101 ggcgacacat atgataagct ggtgggggtg ggatatgtta acagggaata gggaaaatga
     5161 caaagcctaa aataggtgtg gtgatacgac ttcggttcaa ttcaattaat aattaccagt
     5221 ttttttgcat ccggcttttc ctgtgctacg gacactaaca gataataaac tccaggttgg
     5281 agagatggtt cagagatagc cttccggtat aaacctggat tctcagcaaa taaatcaaat
     5341 tcatagataa tggctccggt cggagaaacg attaaaagtc ggatttctcc tggtttccta
     5401 acatccatat caacggtaaa atcaccttta gatggatttg gtcttatgga ggttatccgg
     5461 caagtcggaa attcaccggg aggatcgatc atttcatttg cccccaggga tgatcctcct
     5521 tttggttgta acaggatatc aatccattct ttccgttgcc tgttaaattg atttttcgat
     5581 tcggggacga gctccggatg tttggtaacg aggactgatt tcattgctgt atcgctgtta
     5641 ttctgcatga aaacataggc aaggtcgatc agggcgaaaa cggagtcggc ctgtgttccc
     5701 gggttgaaga tgatcgaatc gagcgaattg attgcctgct ggtaatgctg atccctgatg
     5761 ttgcaatgaa tggaaatcca ttctgcggtt tttccgagca aactgtcgcc cggattttct
     5821 gacaagttat cgcaataagc ttttagtgtg gcgtaatttg aatacaatac cgggttcaat
     5881 gcaaacagct cctttagcga cgccagggca aaccggtttt ccgggaatcc ggcaatgatc
     5941 tgtttaaacc cactgtcggc ggcctggtag ttttccgcct caatttcagc cttggcttcg
     6001 taaaacatgg tttcggcaac atcgtccgat ttgagatagc tttcaccggg gcaccagacc
     6061 ggatcccaat cataataacc cagtgggatc aagcgcaggg acggatcgga atgatcgacc
     6121 cagcagttat tt
//
LOCUS       DAOPNH010000210         6105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_14857_length_6105_cov_6.939339, whole genome shotgun sequence.
ACCESSION   DAOPNH010000210 DAOPNH010000000
VERSION     DAOPNH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14857_length_6105_cov_6.939339"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="lptB"
                     /locus_tag="PKZ74_10740"
     CDS             complement(<1..659)
                     /gene="lptB"
                     /locus_tag="PKZ74_10740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPM93276.1"
                     /translation="MILRTEKIVKKYRQRTVVNDVSVEVKQGEIVGLLGPNGAGKTTS
                     FYIIVGLIKPFSGKVFLDEDEITEEPMYRRAQRGIGYLAQEASVFRKLSVEDNIKAVL
                     EFTRLTKGEQKDKLESLLEEFGLQTVRKSLGIQLSGGERRRTEIARSLAVSPKFILLD
                     EPFAGVDPIAVEDIQNIVSHLKDKNIGVLITDHNVHETLSITDRSYLLFEGKVLKSGT
                     S"
     gene            complement(691..3165)
                     /locus_tag="PKZ74_10745"
     CDS             complement(691..3165)
                     /locus_tag="PKZ74_10745"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPM93277.1"
                     /translation="MIRYFRYIFLLLWMIAIVPAIVFSQETRITGKITDARTGEPIPF
                     VNVYFPNTTTGATSDAEGSYTLITTRPGDSICASAVGYQMQIAHIQKGKIQTIHFVLS
                     ESTTTLHEIQVRPEERWVELLMKLVIKNKEKNNPDRIETYQCQVYNKIRIDINNVDPQ
                     LRERRIFKPIDFVFEHLDTSEIGQKIFLPSLISESVSDYYFRKSPRSTREVIKASRIS
                     GVENQSFTQYLGGLFLGMNIYDNYINVFDKNFVSPVAGFALFTYEYFLEDTVMVDGKT
                     CYQIRFEPKRKQEMTFYGTLWIHDTTFAVKQIDMRVMPDANFNWVNDFYISQSYDQSG
                     SYWTLTRDYRLVDLNPFKGDVIKTFGIFGHRSTIYTDYVFDNPKEDEFYTSATNVMID
                     PGAYRKEDSWWLVNRPDSLTDTEEQIYEMIDSVKNVPAYKYYEKAVLLLGTGYLVTGN
                     FEIGPLYKFVSFNSIEGLRLRAGGRTSNKFSRKLMLEGHAAYGTKDRQWKYAAGMTYM
                     VGKNPRRSVNLSYRYDMEQLGQDPNAFSEDNFFASFFRRSPADKLTMVSELKTSYEHE
                     WFTGFSNAIRFIRREVYSLGDEKFIINKFGKQILDNSLVAGEIQLYTRFAYRERFLYG
                     EFERTSLGTKYPVAELIYSYGIPGFAGSDVEYHRIQFKLKQRFNVANIGWSKYILEAG
                     KTWGRLPYPFLKIHEGNETFFYYTESGNLMNYYEFISDRYASLFYIHHFDGWFLNKIP
                     LMRKLKWREVVHGRAVWGAMTQENLHYSVLPANSGELRKPYFEVGTGIENIFKVGRID
                     AIWRLSHLDKPDAKRFAIFISVNFTF"
     gene            complement(3219..4184)
                     /locus_tag="PKZ74_10750"
     CDS             complement(3219..4184)
                     /locus_tag="PKZ74_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPM93278.1"
                     /translation="MKTYEEIREIAQQTVRAERDAINGLQDSIGDDFVSLVRLILENQ
                     GRVIITGIGKSAIIAEKIVSTLNSTGTPAVFMHAADAIHGDLGIIQKEDLVICLSKSG
                     ETPEIKVLIPLLKHRGNKLAAIVGNPSSYLARSADFVLNSSVPKEACPNNLAPTSSST
                     AQLVMGDALAVALLECRGFTAGDFARLHPGGSLGKQLYLKVSDLSINNEVPRVDADAD
                     ISQVIMEISAKRLGATAVISNGTMVGIITDGDLRRMLEKNTDLKEVRAKDIMTIHPRT
                     IPADSLVVEALALMRNNNITQLPVTENDRYVGVIHLHDILKEGIL"
     gene            4361..6076
                     /gene="recQ"
                     /locus_tag="PKZ74_10755"
                     /pseudo
     CDS             4361..6076
                     /gene="recQ"
                     /locus_tag="PKZ74_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
BASE COUNT         1641 a         1473 c         1436 g         1555 t
ORIGIN      
        1 tgggaggtgc ccgatttaag gacttttcct tcgaagagaa gataggaacg gtcggtaatg
       61 gagagcgttt catggacgtt atgatcggtg atcagtacgc cgatgttttt atctttaaga
      121 tgggagacaa tattctggat gtcttcgacg gcgataggat cgacgccggc aaagggctca
      181 tccagaagga tgaatttcgg gctgacagcc agcgaccggg cgatctccgt ccgcctcctc
      241 tcccctcccg aaagctggat acccaggctt ttgcgaacag tctgaaggcc aaattcctcc
      301 agtaatgatt ccagtttgtc tttctgttct cctttagtca gcctggtaaa ttcaagcacc
      361 gccttgatgt tatcttccac gctcaatttt ctgaaaacgc ttgcttcctg cgccaggtaa
      421 ccgataccgc gctgcgcccg cctgtacatc ggctcctccg ttatctcgtc ttcatccaga
      481 aagaccttac cggagaacgg cttgatcagc ccgacgatga tatagaacga tgtcgtcttc
      541 cccgccccgt tagggcccag caagccgacg atctcaccct gtttaacttc aacggagaca
      601 tcattgacaa cggtgcgctg ccggtatttt ttaacgattt tttcggtccg taggatcata
      661 tctggcaaat ttactgattc tttgaacgca tcagaaggta aaattaacgg aaatgaagat
      721 cgcaaagcgt ttggcgtccg gcttatcaag gtgcgaaagg cgccagatag catctatccg
      781 cccgacttta aatatattct cgatcccggt gccgacttca aaataaggct tcctcaactc
      841 gccgctgttt gccggcagaa cggaataatg caggttctcc tgcgtcattg cgccccagac
      901 ggcacgaccg tgaaccactt cccgccattt cagcttccgc atgaggggaa ttttatttaa
      961 aaaccaaccg tcaaaatgat gtatataaaa taacgaagca taacggtcgc tgataaactc
     1021 gtaataattc atcaggtttc ccgattcggt ataataaaag aaggtctcgt tgccttcatg
     1081 gatcttcagg aacgggtaag gtagccggcc ccaggttttc ccggcctcaa gaatatattt
     1141 cgaccacccg atgttcgcca cgttgaacct ttgcttcaat ttaaactgaa tccggtgata
     1201 ttccacatca cttccggcaa aacccggaat gccataacta tagatcaact cggctactgg
     1261 atatttcgtt ccgagagagg tccgttcaaa ttcaccgtac agaaaacgct cgcgataggc
     1321 aaaacgtgta tataactgta tctcgcctgc aaccagagaa ttatccagaa tctgttttcc
     1381 aaatttatta atgatgaatt tttcatctcc cagagaatat acttcacgac ggataaagcg
     1441 gatggcattg gaaaatccgg taaaccattc atgctcatag ctggtcttca gttcactcac
     1501 catggtcagc ttatcggcag gacttcgcct gaagaacgac gcgaagaaat tatcctcgct
     1561 gaaggcattc ggatcctggc ccaattgctc catatcatag cgataagaaa gattgacgct
     1621 gcgccgggga tttttaccga ccatataggt catcccggca gcgtatttcc attgccggtc
     1681 ttttgtgcca tatgctgcat gaccttcgag catcagcttg cggctgaatt tattgctggt
     1741 tctgccaccc gcccttaacc ttaatccttc tatggaatta aagctgacga acttgtacaa
     1801 aggcccgatc tcgaaattac ccgtaacgag gtatccggta cccagcagca aaacagcttt
     1861 ctcataatat ttatacgcag ggacattttt taccgaatcg atcatttcat agatctgctc
     1921 ttcggtgtcg gtcaggctgt caggacggtt aacaagccac caggaatcct ctttccggta
     1981 ggcacccggg tcgatcatga cattggtggc agaagtataa aattcatcct ctttcggatt
     2041 gtcaaagaca taatcggtat aaatggtcga ccggtggccg aaaattccaa aggttttgat
     2101 cacatcgccc ttgaatgggt tcaggtcgac gagcctgtaa tcgcgtgtca gggtccagta
     2161 agatcctgac tgatcatacg actgggagat ataaaaatcg ttgacccagt tgaaattggc
     2221 gtcaggcatc acccgcatgt caatttgttt aacggcaaat gtcgtatcat ggatccatag
     2281 tgtcccgtaa aatgtcatct cctgttttcg cttcggttca aaccggatct ggtagcaagt
     2341 ttttccatca accataacgg tatcttccag gaaatattca taagtaaaca atgcaaaacc
     2401 agcaaccggg ctgacgaaat tcttatcaaa cacattgatg taattgtcat atatgttcat
     2461 tcccaggaac aaccctccga ggtactgggt aaagctctgg ttctccacac cggaaatccg
     2521 gcttgctttg ataacttccc gggttgaccg gggggatttc ctgaaatagt aatcggagac
     2581 cgattctgag atcagcgatg gcaggaaaat tttctgaccg atctcactgg tatccaggtg
     2641 ttcaaaaaca aaatcaatcg gtttgaaaat cctcctctcc ctcagttgcg ggtcgacatt
     2701 atttatatcg atccggattt tattgtatac ctggcattgg taggtttcaa tccggtcggg
     2761 attgtttttc tccttgtttt taatgaccag tttcatgagc agttcaaccc atctttcctc
     2821 gggcctgacc tgtatttcat ggagtgttgt agtggactcg gagaggacaa aatggatcgt
     2881 ctggatttta cctttctgaa tatgtgctat ttgcatttgg tatccaaccg ctgaggcgca
     2941 gatggagtct ccgggccggg tagtgatcag cgtataactc ccctcagcat cggaagtagc
     3001 cccggttgtt gtattgggaa aataaacatt cacaaaagga atgggctcac cggtgcgggc
     3061 atcggtgatc tttccggtta tccgggtctc ctgggaaaac acgatcgccg gtacaatcgc
     3121 aatcatccag agaaggagaa aaatatatcg gaaataccgg atcatcgggt aaaagtaaat
     3181 tcatcgatcc taaaagaaca accggggacc aaaatcgttc acaagatccc ttcttttaaa
     3241 atatcatgca ggtgaataac accgacatag cggtcatttt cggtcacagg caactgggta
     3301 atattattgt ttctcatcag cgccagtgcc tcgacgacca ggctgtcggc cggaatagtc
     3361 ctgggatgga ttgtcataat atcttttgcg cggacctctt tcaggtccgt atttttctcc
     3421 agcatccttc gcaggtcgcc atcggtaatg ataccgacca ttgttccatt gctgattacg
     3481 gccgtagcgc ccaggcgttt ggccgagatc tccatgatca cctggctgat atccgcgtct
     3541 gcatcaaccc gcggcacctc gttatttatc gagagatccg aaactttcag gtaaagctgt
     3601 ttgcccagtg aaccgcccgg gtgcagccgg gcaaaatccc ctgcggtaaa tccgcggcac
     3661 tccaggaggg ctaccgccag ggcgtcaccc atcacaagct gtgccgtaga actgctggtc
     3721 ggggcgaggt tattcggaca agcctctttc ggcaccgaag aattgagcac gaaatcggct
     3781 gaccttgcca ggtaagacga tgggttcccg acgatggccg caagcttgtt tccacggtgt
     3841 ttcagcaatg ggatcaggac tttgatttcc ggtgtttcac cgcttttcga caggcagatc
     3901 accaggtctt ccttctggat gatgcccagg tcaccgtgaa tggcatcggc agcgtgcata
     3961 aaaacggccg gggtgcccgt ggagttgagt gtcgatacga tcttttccgc aatgatggcg
     4021 cttttgccga ttccggtaat gatgacacgc ccctggtttt cgaggatcag ccggaccagg
     4081 ctcacgaaat cgtcgccgat ggaatcctgc aaaccattga tggcatctct ttcggcgcgg
     4141 acagtttgtt gcgcaatttc cctgatctct tcgtaagttt tcaaaacaat cagtatttca
     4201 gtctgttaat ttttcgagaa aatattttaa aaaaagttaa aaaaatcatg tttgtctggc
     4261 ttttttcaaa aaatcgtata tttataaaaa tataatctgc tatcgatagt tatagatact
     4321 ctgatttact gacaaagata ctcaatttta ggttgcatta atgatagtca aggaagcctt
     4381 atcgatgaag gaggtactga aggatgtctt tggttttgat aattttaaag ggaaccagga
     4441 aatcatcatc aaacatcttc tttcgaggaa agatactttt gtaattatgc ccacaggcgg
     4501 gggcaaatcg ctctgttacc agctgcctgc catgatgtgc gaaggaacag ccatcattgt
     4561 atcacccctc atcgccctga tgaagaacca ggttgacaat ctccggaact acggcactga
     4621 ccataacctg gcccatttca tgaattcttc gcttagtaag tcggagatga aagaggtcag
     4681 gcaagacctg gtggaaggat acactaaatt attatatgtt gcccccgaat cacttaccaa
     4741 agaagacaac gtcgagttcc tgcgcaatat aaacatatcc ttttatgcca tcgatgaagc
     4801 ccattgcata tcggaatggg gtcacgattt caggcctgaa taccgcagga tcaggcctat
     4861 aatcgaaacc atcgggcagg atgtgcccgt cattgccctg accgctacgg ctacgcccaa
     4921 agttcagcag gatatcatca agaaccttga gataccccag tcgacgatct tcaaatcatc
     4981 attcgacagg cctaatcttt attatgaaat caggcccaag acggaaaacg tcgcaaagga
     5041 tgtcattaaa tatatcaaag ccaacctggg gaaatcaggc atcgtatact gcatgagccg
     5101 gaaaaaggtg gaagagatgg ccgagacact ggtggtcaat ggtatcaaag ctttgcccta
     5161 tcatgcggga ttggaatcga atacccgcaa atcaaaccag gaccggttcc tgatggagga
     5221 tgtcgatgtg attgtagcca ctattgcctt cgggatggga atcgacaagc ccgatatccg
     5281 gtatgttatc cattacgata tgccgaaaag cctggaaagt tattaccagg agactggacg
     5341 gtgcggcagg gatgacggcg aaggcaattg catagcgttt tacagttacg acgatatcct
     5401 gaagctggag aaattcatga aagacaagcc tgtagccgag caggagatcg ccaaggagct
     5461 gctgcaggaa acggttgctt acgccgaatc ggccgtatgc aggcataaac ttctcctgca
     5521 ttatttcggg gaagaattcc ccaaggagaa ctgcggatca tgtgacaact gccttcatcc
     5581 caaagcaaaa ttcgagggta aagactacat caaacttgtg atcgaaacgg tgctggccgt
     5641 caaacaacaa ttcaaggcaa aacatatcgt caatatcatc atcggcaaga actctaccac
     5701 gatgaaatct tacaagcaca atgtgctgga agagttcgga aaaggggccg atgaggatga
     5761 acggttctgg aacgctgcgg tccgccaggc gcttatcagc cagttgctgg taaaggatat
     5821 cgaaaattac ggcatcctga aaatcagcaa ggaaggacat aaattcctga agaaaccata
     5881 cagcatcatg ctcacgcatg atcgggacta cgacaacctg gaggaagaag aggatatcat
     5941 ggaaggggcc cacaaaacca gcacggccga taagaacctg ttcgcgatgc tgaaggatct
     6001 gcgcaaagag atctcccgga aggagaacct gcctccgttt gtgatcttcc aggatccgtc
     6061 gcgaaaaagc tcggctattc gatggaagag ctgacgggca tgcat
//
LOCUS       DAOPNH010000211         6042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15062_length_6042_cov_5.747620, whole genome shotgun sequence.
ACCESSION   DAOPNH010000211 DAOPNH010000000
VERSION     DAOPNH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15062_length_6042_cov_5.747620"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKZ74_10760"
     CDS             <1..343
                     /locus_tag="PKZ74_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93279.1"
                     /translation="PAFELPGTAVVDVYAENRFDTKRYLVHYTVFTGVEDKVVPSIQV
                     YPNPVTNVLHVNGMKDAGVKLFNTSGEQVLTLNKFSGSTIDVSELPSGVYIMNVTTAD
                     GQTMRRKIVVY"
     gene            complement(409..744)
                     /locus_tag="PKZ74_10765"
     CDS             complement(409..744)
                     /locus_tag="PKZ74_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93280.1"
                     /translation="MIESTEGWVEDIGSFEMIGGEAVVKIDPVYASSVKLENDYKVFL
                     TPVSEDIVNLVITKKDPESFRVKGVTNDGRPANCSFDYRIVAKDRVRNSGRMEPVDIP
                     DAINIPRIE"
     gene            complement(837..1574)
                     /locus_tag="PKZ74_10770"
     CDS             complement(837..1574)
                     /locus_tag="PKZ74_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93281.1"
                     /translation="MKTVKITCSILLLLAILNLEKTSGQWAFNGTHIFNTNGGYVGIG
                     TSTPVKLLDVLRNATEPTIRIFNTGGTGGATFQMTDYISGADWKFKATAFGGFKIRDN
                     AHALDVVTIEPNTAANSLYIGAGGNVTIGTDIQTSYVKFQSNQNIIHGIAVSGVSTYT
                     SGSCIGVHGETNTISYAGAGGYFIANNNDGDAPGIYGRADGSSTNTYGVASHAYFGAQ
                     ESEHGATPEIFSEVSTAIFPEAPCGSM"
     gene            complement(2151..2771)
                     /locus_tag="PKZ74_10775"
     CDS             complement(2151..2771)
                     /locus_tag="PKZ74_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPM93282.1"
                     /translation="MKMQLILLLYKLFSIMPGKSQSGKPLSKAKKKKSNILRTMVIII
                     VMIIMIVFLIFSVYTPPARKKSQDRPLSSISRSGPIFRKDGTLSVLNARNQTIVNIEI
                     EVADEEAERMRGLMDRISMPENAGMLFIFEEEEQRSFWMKNTFISLDIIYINANKEIV
                     SIQKYTQPKTTTAIPSDAPAKYVLEVNAGFADKFVISAGDKIDFNL"
     gene            2961..3035
                     /locus_tag="PKZ74_10780"
     tRNA            2961..3035
                     /locus_tag="PKZ74_10780"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2996..2998,aa:Asp,seq:gtc)
     gene            3531..4049
                     /locus_tag="PKZ74_10785"
     CDS             3531..4049
                     /locus_tag="PKZ74_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93283.1"
                     /translation="MKTVLLCLALSVSSFWAFSQAQNTEELIRKFGKNSLENGATPYS
                     YCFGVNPKCHASGCSEVTVKTPKDSDVMVTIKTGDTVVRHAYIRSGNSYTFEMPNGTY
                     QVFFYYGTGWNPGKVMKSTGECEVKGGFVTDEYFGKDDPQVLDNTILEYELILQKNGN
                     FSTRPSDAGEAF"
     gene            4086..>6042
                     /locus_tag="PKZ74_10790"
     CDS             4086..>6042
                     /locus_tag="PKZ74_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93284.1"
                     /translation="MSLRRVVLSFLALLASLSAISQGLSCADSDPFCTGTIYEFPAGT
                     TGAAEAGPYYGCLSTQPAPAWYHMKIGNPGPITIYMHSEPQEDIDFACWGPYTDPTSA
                     CPYGLTQSKMVDCSYSTSWQEYCDIPNGQTGQYYILLITNYSQDPCNIIFSQTAGSGT
                     TDCSILPPLVWSDSPLCVGETLHLMAETISNATYSWTGPAGFSSTQQNPEIPNVTLAM
                     AGDYSCIITVFSQSSPPAVTTVVINDLPDASLLNSSQTTCPGDTVMIALQLTGAAPFT
                     LTCFNGTIYTTYEDLYGPVDTIYLAPPGPVTYTFTQVDDTNCTKYLSGTVFTVYNFPP
                     ATGIMSGGETICSGDTSELVFNLTGTPPWEITYLTNGMNPVTVVAEYTPFSVFVAPQV
                     TTVYQFTQLSDLHCEGSASGQVQVSVDYPTGTLSGDNTICAGSGAILLFILSGYPPWT
                     ITYTENGGNGQEVTTYSSPYALTVYPVENTLYEFTLLSDNYCTGSASGQAMITINQPT
                     GVLSGESTICAGESSEITFDLGGVAPWHIIFEQNGSNPQELNANTTPFPVSVTPLLTS
                     IYTFTYFEDGACEGIPSGSAQVIVNPLPLAQAGNDKTIPNGTSTTLNGSVSGGSGSYA
                     VQWEPASKLVNPDILQPQTVNLFESTLF"
BASE COUNT         1606 a         1392 c         1309 g         1735 t
ORIGIN      
        1 tccggctttt gaacttccgg ggactgcagt ggtggatgtt tacgctgaga atcgtttcga
       61 taccaaaagg tacctggttc attacacagt atttacgggt gttgaagaca aagtcgttcc
      121 ttccattcag gtttacccga atcctgtaac gaatgttctg catgtcaatg gtatgaaaga
      181 tgccggtgtg aaattgttca ataccagcgg cgaacaggtt ctgacactga ataaattctc
      241 aggtagcact attgacgtat ctgaactgcc ttcaggagtt tacatcatga atgtaacaac
      301 ggctgacgga cagaccatga gacggaagat cgtggtttat taataagctg ataataaaaa
      361 aaaagagggt gcctcgatga gagcaccctc tttttttata atatgagatt attcaatccg
      421 gggaatattg attgcgtccg gaatatcaac tggttccatc cggccggaat ttctcaccct
      481 gtcttttgcc acgatccggt agtcgaagct gcagttggcg ggccttccgt cgtttgtgac
      541 gcctttaacc ctgaaacttt caggatcttt ttttgtgata accagattca caatatcttc
      601 agatacaggg gtgaggaaaa ctttataatc attttccagt ttaactgaac tggcgtaaac
      661 gggatcaatt ttaacaacag cctcgccacc aatcatttca aatgatccga tatcttcgac
      721 ccagccctct gttgattcga tcattctgaa actgcgatat tcttctttac ctccagctaa
      781 taaggccttt ttaaaggtgt tatagccacc atcggcaaaa acagcccctg catgggtcac
      841 atagaaccgc aaggtgcctc cgggaaaatc gccgtcgaaa cctctgaaaa gatctccggt
      901 gtagctccat gctccgactc ctgtgcccca aaatacgcat gactagccac accgtatgta
      961 tttgtactgc tgccatctgc cctgccatat attccgggtg catctccgtc attattattt
     1021 gctatgaagt agcccccggc accggcataa gagattgtat tggtttcgcc gtgcacacct
     1081 atgcacgagc cggaagtata tgtagacaca ccagaaactg cgatcccatg gattatattt
     1141 tgattggact gaaatttcac ataggaagtc tgtatatctg tgcctatagt aacattgcct
     1201 ccggcaccaa tgtaaagtga attcgccgct gtgttgggtt cgatggtcac aacatccagg
     1261 gcgtgtgcgt tatctctaat cttaaaaccg ccaaaggccg tcgctttgaa tttccagtcg
     1321 gcgcccgaaa tgtaatcggt catttgaaag gtggcgcctc ctgttccgcc cgtattaaat
     1381 atccggattg tcggttcagt tgcatttcgg agcacgtcca gcaattttac gggcgtgctg
     1441 gttccgatac caacataacc tccgtttgta ttgaagatgt gggtgccatt aaaggcccac
     1501 tgccccgagg ttttttcaag gttgagaatg gctagcaata ataaaatgct acaggtgatt
     1561 tttacggttt tcataagaag atgaattagc aatgccttaa agataaggaa tattgtggca
     1621 taatgtatat gaagtaatgt aaaaataaac tttcagagaa atgaatctcc aaaggttgag
     1681 ctatgcggtc tggacagctt gtcccgagtg ctcgggatga ctcgaacccg cgaccttccc
     1741 gaggactcgg gacaggcatt ctaaccaagt gaaatcatag aagaatccat tctaaggttt
     1801 ggtcaatctc tcaatgaaat tcctgttctt ttgatttttc aggaagtgtt cccgtttaat
     1861 ggcctcggat ttatcgataa atgattccga gtattttaaa atccagggtt gtttttttga
     1921 agtatatccg ctctttgcag aatcatgttg tgataatcgt ttatccaggt tataggtata
     1981 agcgatgtaa tagctatcat cagacaaact ctgtatgata tagaccgagt aaatcatagg
     2041 ctaaaataaa aaaacctctg gagagaaatc tccaaaggtt gggcaatgcg gtctggacgg
     2101 gactcgaacc cgcgaccttc ccgaggactc gggacaggca ttctatgtac ttaaagatta
     2161 aaatcgattt tatcaccagc ggaaattaca aatttatcag caaaacccgc atttacctcc
     2221 agaacatact ttgcaggtgc atccgaaggt attgcggttg tcgtttttgg ctgtgtatat
     2281 ttctgaatac taactatttc tttattagca tttatataaa taatgtccag tgaaatgaag
     2341 gtattcttca tccagaatga cctttgttct tcctcctcga aaataaaaag cataccagca
     2401 ttttccggca tacttatcct gtccatcaat cctctcattc tttccgcttc ttcatcggca
     2461 acctcaatct caatgttaac aatagtttga ttcctggcat tcaaaaccga taatgttccg
     2521 tctttcctga agatgggtcc ggatcttgaa atagatgaca aaggacgatc ctgagatttt
     2581 tttctggcgg gcggagtata tacactgaag atgaggaaaa caatcatgat tatcatcaca
     2641 ataataatta ccattgtccg cagaatattt gatttcttct tttttgcctt tgataaaggc
     2701 tttccagatt gtgatttccc gggcataata ctgaatagtt tgtacaaaag taggattaat
     2761 tgcattttca ttttttatct gattgatatg ctttgggata taaaaaaatg agcatacagg
     2821 ttgattgtat tataaaatag tttacttttg cacccttaaa agttctttaa gaattaggtc
     2881 tggtagttca gcttggttag aatgcctgtc ccgagtcctc gggaaggtcg cgggttgaat
     2941 ccataagttc ttaaaatatt ggtctggtag ttcagcttgg ttagaatgcc tgcctgtcac
     3001 gcaggaggtc gcgggttcga gtcccgtcca gaccgcatag cccaaccttt ggagatttct
     3061 ctccagaggt ttttttattt tagcctatga tttactcggt ctatatcata cagagtttgt
     3121 ctgatgatag ctattacatc gcttatacct ataacctgga taaacgatta tcacaacatg
     3181 attctgcaaa gagcggatat acttcaaaaa aacaaccctg gattttaaaa tactcggaat
     3241 catttatcga taaatccgag gccattaaac gggaacactt cctgaaaaat caaaagaaca
     3301 ggaatttcat tgagagattg accaaacctt agaatggatt cttctatgat ttcacttggt
     3361 tagaatgcct gtcccgagtc ctcgggaagg tcgcgggttc gagtcctccc gagcactcgg
     3421 gacaagctgt ccggaccgca tagcccaacc tttggagatt tctctccaga ggtttttttt
     3481 gtcttctcat ttttagtaat ttagtcatct catcaaaagc cagactaaaa atgaaaacag
     3541 ttctcttatg ccttgcactg tcggtttcgt ctttctgggc tttcagccag gcccagaata
     3601 cggaagaact tatccggaaa ttcgggaaga attcactgga aaatggcgct acgccttatt
     3661 cctattgttt cggcgttaat ccgaaatgcc acgcatcggg ctgctccgag gttaccgtta
     3721 aaaccccgaa agacagcgat gtgatggtta cgataaaaac cggcgatacg gtggtcaggc
     3781 acgcctatat ccgttcgggc aactcttaca ctttcgagat gccgaatggc acttaccagg
     3841 ttttctttta ttacggtaca ggatggaatc ccggcaaggt gatgaaaagc accggggaat
     3901 gtgaagtgaa aggaggattt gtaaccgatg aatatttcgg taaagatgat cctcaggttc
     3961 ttgacaatac cattctggaa tacgaactca ttcttcagaa gaatggaaat ttttcgacac
     4021 ggcccagcga tgccggtgaa gcgttttgat catttgctta tttttattga aaattaaaat
     4081 tggtcatgtc actcagaagg gtcgtccttt cattcttagc tttattggct tctctttcag
     4141 ctatttcaca gggcctgagc tgtgctgact cagatccttt ctgcacagga acgatctatg
     4201 aatttcccgc cggcaccacc ggcgctgctg aagccggtcc ctattacgga tgcctttcca
     4261 cacaacctgc cccggcatgg tatcatatga aaataggcaa tcccgggccg attacgatat
     4321 acatgcacag tgagcctcag gaagatatag actttgcatg ctggggaccc tataccgatc
     4381 cgacctcggc ctgcccatat ggactgacgc agagtaaaat ggtcgattgt tcctattcca
     4441 cgagctggca ggaatactgc gatatcccaa atggccagac aggacagtat tatattctcc
     4501 tgatcacgaa ttattcacaa gatccatgca atatcatctt ctcacagact gcaggatccg
     4561 gcaccaccga ttgttccatc ttgccaccac tggtgtggag cgacagccct ttgtgcgttg
     4621 gagaaacact acacctcatg gcagagacca tttccaatgc cacctattca tggaccgggc
     4681 cggctggatt cagctcgaca caacaaaatc cggagatccc caatgtaaca ttagccatgg
     4741 caggagatta ttcctgcatt atcactgtgt tcagccagtc tagcccgccg gccgtaacaa
     4801 ctgtcgtgat caacgatctg ccggatgcat ccctgctaaa tagcagccag actacctgtc
     4861 ccggcgatac tgtcatgata gctctgcagc ttaccggcgc cgctcctttt actcttacat
     4921 gttttaacgg gaccatatat acaacctacg aggaccttta cggtccggta gacaccattt
     4981 atctggcgcc acccggccct gtcacttata cgttcacaca agtggacgat actaattgca
     5041 cgaagtacct gtcgggaacg gtcttcaccg tatataattt tcctccggca acaggaataa
     5101 tgtcgggagg cgaaacgatt tgtagcggcg atacttccga gctggtattt aatcttacag
     5161 gaacgcctcc ctgggaaatt acttacctta ccaatgggat gaacccggtt acggttgtgg
     5221 cggaatatac tcctttctca gtttttgtag cgccccaggt tacaaccgtt taccagttca
     5281 cacagttgtc agatctgcat tgtgagggct ccgcatccgg gcaggtgcag gtttcggtgg
     5341 attatccaac aggaaccctg agtggcgata atactatatg cgccggatcg ggagccattt
     5401 tactgttcat cctttcagga tatccaccct ggactattac ttatacggaa aatggaggaa
     5461 acggacagga ggtaacgact tactcatctc cttatgctct tacggtttac ccggtagaaa
     5521 ataccttata tgaatttact ttgctgtcag ataactattg taccggctcg gccagcggcc
     5581 aggccatgat cacgattaac cagcctacag gtgtattgag cggtgaatct acgatctgtg
     5641 ccggggagtc ctctgaaata acctttgacc tgggaggagt cgccccgtgg cacattattt
     5701 tcgaacagaa cggaagtaat ccgcaggaac tcaatgccaa taccactccc ttccctgtat
     5761 cggtcactcc tttattaacc tccatttata catttaccta ttttgaagat ggggcttgtg
     5821 aggggattcc ttcaggctct gcacaggtta ttgtcaatcc gttgccgtta gcgcaggcag
     5881 gcaatgataa gactattccc aatggcacat ccacaacttt aaatggatcc gtatcgggag
     5941 gttccggtag ttatgccgtt caatgggaac cggcttctaa gctggttaat cctgatattc
     6001 ttcagcctca gaccgttaat cttttcgaat ccaccctttt ta
//
LOCUS       DAOPNH010000212         6022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15136_length_6022_cov_6.832747, whole genome shotgun sequence.
ACCESSION   DAOPNH010000212 DAOPNH010000000
VERSION     DAOPNH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15136_length_6022_cov_6.832747"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..945
                     /locus_tag="PKZ74_10795"
     CDS             343..945
                     /locus_tag="PKZ74_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93285.1"
                     /translation="MKNLFVLFLVLMPFSFCEAQIMPRTDYIVIELGIDLDLGINYDQ
                     ELQYDIDLFDDGIIDYKIINNGIIVDPLADLHAFEKIIPYNGNSVAFYSIDSSFCGDE
                     LWNTYYTAINLLPGDTISDDLIFDTIPLSAFENESAEISDNGQWCYPNYVYHPSSHLI
                     TPLKLNSGEGVPGNKSYSLGGLYHIRANRSWNRRCSLRYP"
     gene            complement(1094..1369)
                     /locus_tag="PKZ74_10800"
     CDS             complement(1094..1369)
                     /locus_tag="PKZ74_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93286.1"
                     /translation="MQPNAPSAAPWAGHMIVDYRLSTFDFRIFPSAIDNGQWTMDNGQ
                     SSGPAWAMRVCYAPMPLYHNDFLNNDFIIQLDLNQIISTFNDFNIWK"
     gene            1454..1711
                     /locus_tag="PKZ74_10805"
     CDS             1454..1711
                     /locus_tag="PKZ74_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPM93287.1"
                     /translation="MRAANFTEFRTRLKKFLDDVENNHETLIIKRKTGNGAVLISLDE
                     YNSIMETVHLLSSKINADRLYESIQQMKSGTVVKKDLIEEQ"
     gene            1708..1971
                     /locus_tag="PKZ74_10810"
     CDS             1708..1971
                     /locus_tag="PKZ74_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPM93288.1"
                     /translation="MKITFSRNAWEDYTSWQSEDKRMLKKINELIKDIQRIPFEGMGK
                     PEPLKYDLSGLWSRRIDREHRLVYQANETELLIYSCRYHYDLY"
     gene            2095..2295
                     /locus_tag="PKZ74_10815"
     CDS             2095..2295
                     /locus_tag="PKZ74_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93289.1"
                     /translation="MIIWLVLFFGLSCQAFAKAGVANGGFELLLAIIGFLLLVTGLYT
                     GIEYLIKNGRNLFNRFRAILKD"
     gene            2452..3882
                     /locus_tag="PKZ74_10820"
     CDS             2452..3882
                     /locus_tag="PKZ74_10820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPM93290.1"
                     /translation="MGQHLIDKIRRNRGMAIYAVLATYAVLAAFIITFFNGTGDSGDS
                     IHHYLFARYAPQHIELFFDHWAKPLFVLLASPFAQFGFTGMKIFNALAGLFTIWFTYR
                     TVQLLNLRNPLLVTLLLIFAPMYFVQTFSGLTEPLFALFISIGLYFVVREKFVLSFLI
                     ISFLPFIRSEGLILIGVFALYAIVKNQWRPLPWLLTGHILYSVAGFFVHGDLLWVFTR
                     IPYARLASTYGSGTIFHFAEKLNYVIGIPIFILFWAGVLSIILKLVRRQISKEIFILV
                     FLGFLSYFIAHSLFWYFGIFNSMGLQRVLIGVMPMIAIISLTGFNFLTEELARNHRVT
                     RKISQIALMSYILIFPFLSNPAAIKWDTDLSLAEDQVAATGVKEFIISHYSITTPRLI
                     YSHPYLSEALQVDHFDQEKRLDLNKENLSRLRPGDLIIWENWFAVVENGISKESLEQN
                     PDLINIFNTTNNKGKREIQFIIFKKN"
     gene            4021..5508
                     /locus_tag="PKZ74_10825"
     CDS             4021..5508
                     /locus_tag="PKZ74_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM93291.1"
                     /translation="MKEKVVIIGGGVAGLSAGIYSAMNGFDTEIYEMHSVAGGQCTAW
                     DRDRFRFDYCLHWLVGTSKGPFHEIWRETNVLNDQTEIIDHEIHSQIIDNKGERFTIY
                     ADINRWEEYLLKLSPEDEKPIRSMCREMRKSARLEPFTSAPELRSPLEYLRLLPSMIP
                     LMFVVQKFGRLTCAEYFDKLNFKSEKIRTAFHSLYGDRNFSALAFIFMLGWFNQKNAG
                     YIKGGSYPLAQRMRERYESLGGRIVFRKRVEKIIVEKNIAKGIVLTDGTVIKADHVIS
                     AADGFSTIYKMLDGKYTSKQVEHAYKNWELFPPLVQVSFGVGKEVKADAPILLNFSKG
                     VKIGMTELEYGFSLMNYSYDNTMAPEGKTTIVMRYESPWKLWENLEGEAYRAEKEQIK
                     KDAVACLESIHPGISGSIEVIDVATPKTDVKYTGVKDGAYEGFLPTRENMMKGLRMQL
                     PGLKNFYMAGQWLFPGGGLPPSAQTGKWAVQMICKKKRQKFISNR"
     gene            5531..>6022
                     /locus_tag="PKZ74_10830"
     CDS             5531..>6022
                     /locus_tag="PKZ74_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93292.1"
                     /translation="MKQTEEIIFNALRTYSNVHRGSGYNSMVTTENYEQAKLKVLGFM
                     GLDKKSHKVVFCSPRQAQIITSGMKPGSYQVLSSIDTGLAIGLRAITAKRKALSMVPK
                     ITGGGTTRLISGDWVVWAKAPDRHEAGTPSVINAIAFTSALDMKKKTNAGELIQIKPE
                     NTEP"
BASE COUNT         1787 a         1264 c         1323 g         1648 t
ORIGIN      
        1 acgacattat ggtagaaaaa ggaaacacca caaatccatt aagtcccgcc agggacgaca
       61 ttatggtaga aaaaggaaac accacaaatc cattaagtcc cgccagggat gcaattatgg
      121 taggaaaaat aacaagcccg ccggataatt tgaatattta aaataattat atttgaggag
      181 attataccaa gataatcata tacctaagaa aatcacgcag tgaaatatca gtcagtgaaa
      241 aatacataca tcttgagtgg tataaaatca actagaataa tgataagtta aatataaagt
      301 caatagactt tgcacaaacg ttatgcccca atacttatat ctatgaaaaa tttattcgtc
      361 ttatttttgg tcctcatgcc tttctctttt tgcgaggctc aaattatgcc tagaacagat
      421 tatattgtta ttgaacttgg tattgatctg gatttaggga ttaattatga tcaggaattg
      481 cagtatgata ttgatctgtt tgacgatggt attattgatt ataaaatcat caataacggg
      541 ataattgttg atcctctggc ggacctgcac gcttttgaaa aaataattcc atacaatggg
      601 aatagtgtcg ctttttattc tattgattct tcattttgtg gagacgaact ttggaataca
      661 tattatactg caatcaattt attgcccgga gatacgataa gtgacgatct catttttgat
      721 accatcccct taagcgcttt cgaaaatgaa agtgccgaaa tttcggataa tgggcagtgg
      781 tgctatccaa actatgtata tcatccatct tctcatctta ttacacccct taaattaaat
      841 tcgggggaag gggtacctgg aaacaaaagt tattcactgg gaggcttata tcatattaga
      901 gcgaatcgtt cttggaacag acgttgttct ctcagatatc cctgaaaatt taaatactga
      961 ttgtttgaaa atatacccca atccatccac aggtattgtt catttcaggt atgacagacc
     1021 atttaataag attcagctta taaccgttac agatataacc ggaaaacaac tgaaacagtt
     1081 tcagaatctg aaatcatttc cagatattga aatcgttaaa agtggatatt atttgattaa
     1141 gatccaattg gataatgaaa tcattattaa gaaaatcatt gtggtataaa ggcatcgggg
     1201 cataacaaac gcgcatcgcc caggccggac cagaggattg tccattgtcc attgtccatt
     1261 gtccattgtc gatggccgaa gggaagattc gaaaatcgaa cgtcgacaat cgataatcga
     1321 caatcatgtg cccggcccag ggcgctgcgc tcggagcgtt aggctgcatg tttattattt
     1381 ttaattttgc ttataagtaa ttaataaagt acgtataatt gtactcgttt aataacttaa
     1441 ataatttata aaaatgcgag cagctaattt cacagaattc agaacccgct taaaaaaatt
     1501 cctagatgat gtcgaaaata atcatgaaac attgattatt aaaagaaaaa cgggcaatgg
     1561 tgcggtattg atctcacttg atgaatacaa ttccataatg gaaactgtcc atcttctcag
     1621 ttccaaaatc aatgctgaca gattatatga atctattcag caaatgaaat ctggtacagt
     1681 agtcaaaaag gatttaattg aagaacaatg aaaatcactt tttcgagaaa cgcctgggaa
     1741 gactatactt cctggcaatc agaagataaa aggatgttaa aaaagatcaa tgagttaatt
     1801 aaggatattc agagaatacc ttttgaggga atgggtaagc cagagccctt gaaatatgat
     1861 ttatcaggtt tatggtccag acgaattgac cgagagcata gattagttta ccaagcgaat
     1921 gaaaccgaat tattaatcta tagctgcagg taccattatg atttatatta aacacgcagc
     1981 ctaacggggc gcttcgctga gcattatatg ttcttaaaaa ctttttgtcg attcgaaaag
     2041 taaaattatt atgtaccttt aaccgtgaaa aaaatcagtg aaatgaacaa ggcaatgatc
     2101 atctggctgg tcctcttttt tgggctatcc tgtcaggctt ttgcaaaagc tggtgttgca
     2161 aatggcgggt tcgaactttt gctggcaatc atcggattcc ttcttttggt gacaggatta
     2221 tatacgggaa tcgaatatct gataaaaaac ggccgaaatc tgtttaacag gttcagggcg
     2281 attttgaaag attgaatcta caattcattc agaccttccg gtgattttat ggttttcgtt
     2341 tgttcctccc ggataatctg atctcaggat atttataccg ggactgtaaa tccgttatca
     2401 gctgcaatcc cggttttgat actcccttca tttaaatatc tttgtaaaat aatgggtcaa
     2461 catctaatcg ataaaattcg cagaaaccgg ggaatggcca tttatgcggt attggccaca
     2521 tacgccgtac tggcagcttt catcatcact ttttttaacg gtaccggcga ttcgggcgac
     2581 agcatccatc attacctttt cgcacggtat gccccgcaac atatcgaact tttcttcgac
     2641 cactgggcca agccactgtt cgtacttctt gccagcccat ttgcccaatt cggatttacg
     2701 gggatgaaaa tattcaatgc ccttgccggg ttattcacca tctggttcac ctacaggacg
     2761 gtacaactat tgaacctgag gaatccgctg ttggtaacgc tattgctgat cttcgcccct
     2821 atgtatttcg tccagacatt ttcaggcctt accgagccat tgtttgccct gtttatatct
     2881 ataggcctgt attttgtcgt aagggaaaaa tttgtacttt cctttctgat catttcgttt
     2941 ctccccttta tccgttcgga agggctgatc ctcataggcg tttttgcatt gtatgccatt
     3001 gtgaagaatc aatggcggcc actaccctgg ctgctcacag gccatatcct ctattcagtt
     3061 gccggctttt ttgtgcacgg cgacctgctc tgggtcttta cccggatccc ctacgcccgg
     3121 ctggccagca cctacggcag cggaaccatt ttccattttg cagagaaact gaattacgtt
     3181 atcggcattc ccattttcat cctcttctgg gcaggtgtcc tcagcatcat cctgaaactg
     3241 gtccgcaggc aaatatcaaa agagattttc atcctggttt tcctgggttt cctttcgtat
     3301 tttattgcac acagcctgtt ctggtatttc ggcattttta attcgatggg cttgcagcga
     3361 gttctgatcg gcgttatgcc aatgatagcg attatttccc taacgggatt taattttctg
     3421 acggaagaac tagcccggaa tcaccgggta acccggaaaa tttcacagat tgcattgatg
     3481 agttacatcc tgatattccc ctttctttcc aatcctgcgg cgatcaaatg ggatactgac
     3541 ttaagcctgg cggaggacca ggtggctgcc actggcgtta aagaatttat cataagtcac
     3601 tacagtatca ccacaccccg gttgatctat tcgcatccct atttaagcga agcattgcag
     3661 gtcgaccatt ttgaccagga aaagaggctg gatttgaata aggaaaacct cagccggttg
     3721 cggccgggcg acctcatcat ctgggaaaac tggtttgccg tagtggaaaa tgggatctca
     3781 aaagaatccc tcgaacaaaa tcctgatctg ataaacattt ttaatacaac aaacaacaaa
     3841 ggaaaacgag aaattcaatt tatcattttt aagaaaaatt gattgacagt aaaaagaata
     3901 gtatcgaaat cctgttttca tcccggataa aatgaaattc accggtttat cccgttcatt
     3961 caccggaaaa tcattcaggt ttaaagcagg tgcagatact tttgcgcaaa aattaatcac
     4021 atgaaagaaa aagttgtcat tattggcgga ggtgttgccg ggttgtcggc cggaatttat
     4081 tccgcaatga acggattcga caccgaaatc tacgagatgc attcggtagc cggagggcag
     4141 tgtacggcgt gggaccgtga ccggttccgg ttcgattatt gtctccactg gctggtggga
     4201 acctccaaag gccctttcca cgaaatctgg cgggagacca atgtgttgaa cgatcagacc
     4261 gagattatcg atcatgagat ccattcccag atcattgaca acaaaggaga aaggtttacg
     4321 atttacgccg acatcaaccg gtgggaagaa tatttgctga aactctcccc cgaggatgaa
     4381 aagccgatcc ggagcatgtg cagggagatg cgtaaaagcg ccaggctgga gcctttcaca
     4441 tcagccccgg aactgcgcag cccgctggaa tacctgcgcc ttctgccttc catgatccct
     4501 ttgatgttcg ttgtgcaaaa attcggccga ctgacctgcg ccgaatattt cgacaaactc
     4561 aatttcaaaa gcgaaaagat caggacagct tttcattcgt tgtatggcga ccggaatttt
     4621 tccgccctgg cgttcatatt catgctgggt tggttcaacc agaagaatgc gggttacatc
     4681 aaagggggct cctaccccct ggcccagcgg atgcgggaaa gatacgaaag ccttggcggc
     4741 aggatcgtat tcagaaaaag ggtggagaaa attatcgtag agaagaatat agcgaaagga
     4801 atagtactga cggatggcac tgtcattaaa gccgaccatg tgatcagcgc agccgatgga
     4861 ttttcaacga tctacaagat gcttgacggc aaatacactt cgaaacaagt tgagcatgca
     4921 tataaaaact gggaattgtt cccgcccctg gtgcaggttt ctttcggtgt cggcaaagag
     4981 gtaaaagccg atgcccctat tcttctgaat ttctccaaag gggttaagat cgggatgacc
     5041 gagctggaat atggattctc gctgatgaat tattcttacg acaacaccat ggcacccgag
     5101 gggaaaacga ccatcgtcat gcgatatgaa agtccgtgga aattatggga aaacctggaa
     5161 ggggaggctt accgtgcaga aaaggaacaa ataaagaagg atgccgttgc ctgcctggag
     5221 tcgatccacc ccggtatatc gggctccatc gaagtgatag atgtagccac acccaaaacg
     5281 gatgtcaaat acacaggtgt taaagacggg gcctatgagg gatttctgcc caccagggaa
     5341 aacatgatga aaggattgag aatgcagttg ccgggattga agaacttcta catggccggg
     5401 cagtggctgt tcccgggagg cggactgcca ccgtcggcgc agaccggaaa atgggcagta
     5461 cagatgatct gcaagaaaaa gaggcaaaag tttatatcaa accgttaaca gacctgaacc
     5521 aaccaaaagt atgaagcaaa cagaagagat cattttcaat gccttgagga cttactcgaa
     5581 tgttcaccgg gggagcggat acaattcgat ggtgaccact gagaattatg aacaggctaa
     5641 gctcaaagtt ctcggtttca tgggtttgga taagaagagc cataaggtcg ttttttgttc
     5701 accccgccag gcccagatca ttacctccgg catgaagccg ggcagttacc aggttctttc
     5761 cagtatcgat acaggcctgg ccatcggcct ccgggctatt accgccaaac gaaaagccct
     5821 ttcgatggta cctaaaatta ccggcggagg cactacgcgc ttgatttcag gcgactgggt
     5881 cgtttgggca aaggcgcctg atcgccatga agcgggaaca ccatcagtaa tcaatgctat
     5941 cgcttttacg tcggcattgg atatgaaaaa gaaaaccaat gccggtgaac taatccaaat
     6001 taaaccggag aatacagagc cg
//
LOCUS       DAOPNH010000213         5970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15339_length_5970_cov_6.250380, whole genome shotgun sequence.
ACCESSION   DAOPNH010000213 DAOPNH010000000
VERSION     DAOPNH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15339_length_5970_cov_6.250380"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2727)
                     /locus_tag="PKZ74_10835"
     CDS             complement(181..2727)
                     /locus_tag="PKZ74_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM93293.1"
                     /translation="MQLARDGKIYCGPKEEPGPNADQTYLSVIHKPWIRGTGCMYEEN
                     AVSIYPNECVYSFINILVDYLFRFEWIGETCQGYPIQFKPNFIPTPQTIQWNFDDGPG
                     SSSWQLSPTYTFKNPGVHEVKVDVWYPSGRYEHTSREIEISPSPLPLLGNDTLICNGS
                     TITLNANCDADFFSWSTGQFGVSSITVSDSGSYWVRGRFNNSGCMGYDTIHIGFHQTT
                     IIDETNLNITPTTCNGASGSITGLTAIGMPPFFYGWKDLSEVEVGTDIEVFDLPAGQY
                     YLTITDANGCETTSDIYTIEDAGNLQVLDVELIQPHCGRPDGEIVVHGFNPSGSALQY
                     SINDGDSYQADSVFSGLVDTSYVVRVTDGGGCFGFYIDNPVVLEDIPGPQVVEVNVTD
                     ETDFLGNGAIEIIASGSTPVIYYSIDSGATYQSNNGTFNNLGSGIYDLLVWDENGCDT
                     AFTVEIQNNILTWLHAVTGEEGICEASTALIPVNVDNFNNVAEFHLKLGYNADNIECE
                     GFANVNVQLLDSLTGWVDQATGEIHLTWNGEFPLTFSQSELATDLVFTTKNPGQGELS
                     WYTGETESYFTNSSGNLIPAEFSTGQVTIYDPPEIILDQSKTVCTGQFANLMSIAVGN
                     QPPITYRWIYPAGDSTSTDPLFFSITPADAGLYTLLATDRVGCTDQKSIQLIVSENPV
                     AAFHGTDTLEMHMGDVLDAGEGMASYRWNTADSMQSIVIHAEGKYQVEMESQIGCTGR
                     DSIYIKIIEDEIPPESHIFIPNAFSPNGDGVNDVFVMKCDGLSIVDCRLSIFDRWGGE
                     VFDSNNISQGWDGKKNSNPCPGGVYVYKIVFSVDGVPGSQERVGTVMLVR"
     gene            complement(4178..>5970)
                     /locus_tag="PKZ74_10840"
     CDS             complement(4178..>5970)
                     /locus_tag="PKZ74_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93294.1"
                     /translation="IDIVMHPAKPDTLYAAIVPPVRTAHPFPHGGPGSGIYRSYDGGD
                     TWTLLTNGLPAASAQVGRIGIDISASDPDILYAIYADNIGYFDGLYKTVNGGNSWFRT
                     SDEELADCYASYGWWFGRIAVDPQDPDVAYVIGFDLYKTIDGGNNWSYISSDVHVDQH
                     DIVIHPDNTDLLVLGNDGGVYFSYNGGYNWDFCDNLPITQFYTCEADEQYPFRLYGGT
                     QDNGTNRTFTGALDNWMMIYGGDGFHVLVDPVNNSYVYAEYQYGNLARSTNGGTSFTS
                     AMSGISGSDRKNWNTPVVFDPSDPQILYYGANRLYRSTNRAVSWQAVSPDLTGGSGSG
                     SVVYGPITTISVSPLNSQVIYAGTDDGFVWRTTDGGQEWADISSGLPLRWITRVAADP
                     YDEDVVYVTLSGYRYDSSLPHVFRSADSGGTWDDISGNLPEAPVNDIIIDPALDSTIY
                     LATDFGVFVSRNLGQGWQMLGDNLPNVPIVDLDFHQPTRTLIAATYGRSMYTFDVDQT
                     VGVSNLNDLPEQILLYPNPAKDYINLNHRFNENIHYSINDLSGKEVKKGRLAAKYERH
                     SLIINDLKPGSYIFKLAGHNRVITGKFEKL"
BASE COUNT         1634 a         1419 c         1395 g         1522 t
ORIGIN      
        1 gatcgtaaaa ttattattaa ttgaaatgcg gaggtcacag gatgcacaat ttttatgagg
       61 aaagtcaaaa gggtgaagcg tgaacggtga agggtgagcg gtgaagcgtg aagcgtcaga
      121 caaaagaatt aggaaatgtg ttaattaact cattggctaa tttccaaatt tccaaattga
      181 tcatctcacc agcatgactg ttcctactct ttcctggctt cctggaacac catcaaccga
      241 gaaaactatt ttgtaaacat acaccccgcc tggacaaggg ttgctgtttt tctttccatc
      301 ccatccttgg gaaatgttat ttgagtcaaa aacctcccca ccccaccgat cgaaaatcga
      361 caatcgacaa tcgacaatcg acaatccatc acacttcatc acaaacacat cattcacccc
      421 atcgccattc ggactaaacg catttgggat aaaaatatgc gattccggtg gtatttcgtc
      481 ttctattatt ttgatatata tggaatctcg accggtgcac ccaatttgag actccatctc
      541 aacctggtac tttccttcag catggatcac aatgctttgc atcgagtcag cggtgttcca
      601 acgataggac gccattcctt cccctgcatc caaaacatct cccatatgca tttccagcgt
      661 gtcggttcca tgaaaagccg caacgggatt ttcgctgacg atcagttgga tacttttctg
      721 gtctgtacag ccaacgcggt ctgtggcgag gagcgtgtag aggccggcgt cagcgggggt
      781 tatgctgaag aatagcgggt cggtgctggt gctatcgccc gctggataaa tccagcggta
      841 agtgatcgga ggctggttgc cgacggcaat gctcatcagg ttggcaaact gcccggtgca
      901 aacggttttg ctctgatcca ggatgatctc cggcggatcg tagatcgtga cctgcccggt
      961 actgaactcg gccgggatga gattgccgct gctgttggtg aaatacgact ccgtttcgcc
     1021 cgtgtaccag gaaagctctc cctgccccgg gtttttcgtg gtgaaaacca ggtcggttgc
     1081 gagttccgac tgactgaaag tcagtggaaa ttcaccattc caggtcagat gaatctctcc
     1141 cgtggcctga tcaacccaac cggtaaggct atccaacaat tgaacattaa cattagcaaa
     1201 gccttcgcat tcaatgttat cggcattata tcccagcttc aggtgaaatt cagctacatt
     1261 attaaaattg tccacattaa cgggaatcaa tgctgtagaa gcttcgcata ttccttcttc
     1321 gccggtaacc gcatgcaacc atgtcagaat attattctga atttcaacag taaacgcggt
     1381 atcacatcca ttttcatccc aaactagtaa atcgtaaatc ccagatccta gattgttaaa
     1441 ggttccattg ttcgattgat aagtcgctcc gctatcgatc gaataataaa tcaccggagt
     1501 agagccagag gcaatgattt caatcgctcc attccccaaa aaatccgttt catcggtaac
     1561 attcacttca acaacctgcg ggccggggat atcttccaaa acaaccggat tatcaatgta
     1621 aaatccaaag cacccacctc catctgtaac ccgcaccacg taactcgtat ccaccagccc
     1681 ggagaacaca ctgtcagcct ggtatgaatc gccatcattg atcgaatact gcaatgcaga
     1741 acctgacgga ttaaatccat gaacaacgat ctctccatcg ggcctgccgc aatgaggctg
     1801 gatgagttca acatccagaa cctgcaggtt gccggcatct tcaatggtgt atatatcgga
     1861 tgttgtttcg caaccatttg catcagtgat agttaaataa tactgcccgg caggaagatc
     1921 aaagacttca atatcagtac caacctcgac ctcggagagg tccttccatc cgtagaaaaa
     1981 cggtggcata ccaatcgccg tcagcccggt aattgagccg cttgccccgt tgcaggtggt
     2041 gggggtaata ttcagattgg tttcgtcgat gattgttgtt tgatggaatc caatgtgaat
     2101 ggtatcatag cccatgcagc ctgagttgtt gaaccgtccg cggacccaat agctgcctga
     2161 atctgaaacg gtaatcgatg aaacgccgaa ctggccggtt gaccaggaga agaaatcggc
     2221 atcgcagttt gcattcagag taattgttga gccattgcag atcaaagtat catttccgag
     2281 gaggggaaga ggggaagggg agatttcgat ttcccgagat gtgtgttcat atctccccga
     2341 gggataccag acatcgacct ttacctcatg aacaccggga ttcttaaaag tataggtcgg
     2401 tgaaagttgc catgaggagc ttcctggtcc gtcatcaaag ttccattgaa tagtctgggg
     2461 agtaggaatg aagttaggct tgaactggat cgggtaaccc tggcatgtct cgccaatcca
     2521 ttcgaagcga aagaggtagt cgacgaggat atttataaaa ctatagacgc actcattagg
     2581 gtaaatactc actgcatttt cctcatacat gcaacctgta ccccgaatcc agggtttatg
     2641 tatgacgctt agataagttt gatcagcatt cgggcctggc tcttcctttg ggccgcaata
     2701 gattttccca tcccttgcca actgcatgct atggagattt gcattatgat aaattttcaa
     2761 tgcggaattt ataaattcag ccgaatcagt aacatactgc atatcatatt gatatagtac
     2821 agtagtatca aaatttgtaa aataagatat atataaatat ttggaatctg gtgaaaactc
     2881 taatccttcc gccgcttcat ttagctcttc aaccgggcct ttcatagtat acaggcagga
     2941 cacctctcca tttagagcat taaatccgca tatttctata ttttggtgat agcttgaaac
     3001 caaatatctt ttatcggatg aaatcttaat atatccccaa tggttctcac ggtcaggcat
     3061 gacggagatt acaggatttg gatttaaacc gttatggtcc accaggaatg ctgcatatgc
     3121 atcctcaata tatttcctgg ttatcaccca tacagcatcc gaattttcct tccttactat
     3181 ggcgacccgg tcgctgacat cccaaccggc atcaatttca atatttttat ccagaacgac
     3241 tcctcctaaa ccctggttta tatcgataat agaataatat aatccatatt gaggccatcc
     3301 ggactccatt gcagttatgt aataataaat accagcacgg cccggccatt caacgattgc
     3361 tgcacctgaa aggcccggcc gaagcatttc ggtaccattg ggcataatac cgtaacttgt
     3421 aaaaatattt tcgttatctg cactaaataa ataattgcct gatgaatcgc atattgtcga
     3481 acctatgtga ccattaagta cctgtgaatc atctagaaca acaggctctc cggaattaaa
     3541 atccagcccg cacagctgag gaaaatacca gatattggct tcgttctggg cgtagatgcc
     3601 gtttgataat aggagcgaca ggactataag acggtaggac ggtaggaaga aatgacgata
     3661 ggacggtaag acggtaagac aataggatct aaggaatgca ggaaagtgaa aatttaattt
     3721 cagggttttc atcttacatc attatgaaga ttgttgtgga tgaaatgatt ggcataaaag
     3781 taggatgttt aggggagaaa tgcaagggaa aagtcggcta tcgtctgccg gcagttccct
     3841 cgctacgctt gccaatgcca agttagtaaa tttcttctga attgctctcc ggttgagagc
     3901 cctatttata acaaaaagga cgggaaagaa aaaaagcccc tccctttgga aatattggaa
     3961 tattgcgaaa aaccttcaaa gggaggggtt ggggagggaa accggaaata catctttccc
     4021 ctccccttaa tcccctccca tgctgatatt accttgaaaa accggattat cgcgggaggg
     4081 gaaaattgtg cgggtgccat cgcctttgct acaaatgtga aactcctccg gagtaatgaa
     4141 aagctaatgt catccaatat tgtggagttt gtaaaattta cagtttttca aattttccgg
     4201 tgataacacg gttatggcct gctaacttaa aaatataact acccggcttc agatcgttga
     4261 taatcagaga atgtctttcg tattttgccg ctaatcttcc ctttttgacc tcttttccgc
     4321 tcaaatcatt gattgaataa tggatattct cattaaaacg atggtttaaa ttaatgtaat
     4381 ccttagcggg attaggatag aggaggattt gttccggaag atcatttaaa ttacttactc
     4441 caaccgtctg atccacatca aacgtgtaca tggaacggcc gtaggtagcg gcaatcaagg
     4501 tccgtgtggg ctggtggaaa tcgagatcga cgatgggaac gttggggagg ttatcaccca
     4561 gcatttgcca gccctggcca aggttgcgcg aaacaaatac gccgaaatcg gttgccaggt
     4621 aaatcgtcga atcgagcgcc gggtcaatga tgatgtcgtt gacaggggct tcgggcagat
     4681 tgccggagat gtcgtcccag gtgccgccgc tgtcagcgct tctgaacaca tgcggcaaac
     4741 tgctgtcgta ccggtatccc gaaagggtaa cataaacaac atcttcatca taaggatcgg
     4801 cggctacccg cgtaatccag cgcaacggaa ggccggagga gatatcggcc cattcctgtc
     4861 cgccatcggt tgtacgccat acaaaaccgt cgtcggtccc ggcataaatc acctggctgt
     4921 tcaatggtga gaccgaaatg gtcgtaatcg gcccgtaaac aaccgatccg ctgccgctgc
     4981 ccccggtgag atcggggctg accgcctgcc aggagacggc ccggttcgtc gatcgataaa
     5041 gccggttcgc gccatagtaa aggatttgag gatcagatgg atcgaaaacc accggcgtat
     5101 tccagttctt ccggtctgag ccgctgatac cgctcatggc cgatgtaaaa ctggtacccc
     5161 cgttggtcga acgggccagg ttgccgtact ggtattcggc atagacataa ctgttattca
     5221 ccggatcgac gagcacatga aaaccgtcgc cgccataaat catcatccag ttatccaaag
     5281 ccccggtaaa ggtccggttg gtgccgttat cctgcgttcc gccatagaga cggaacgggt
     5341 actgttcatc cgcctcgcag gtataaaact gggtgatggg caggttatca cagaaatccc
     5401 agttatatcc gccattatag gagaaataga ccccgccgtc gtttcccagg accagcaaat
     5461 ctgtgttatc gggatgaata acgatatcgt gctgatcgac atgtacatcg gaggaaatat
     5521 atgaccagtt gtttccgccg tcgatggttt tgtaaaggtc aaagccgatc acataggcaa
     5581 catcagggtc ctgcggatca acggcaatgc ggccgaacca ccagccgtag gaggcatagc
     5641 aatcggccag ttcttcatcg ctggtccgga accaggaatt gccgccgttc accgttttgt
     5701 acaatccatc gaaatagccg atattatccg cataaatggc atagagaata tccggatcgg
     5761 atgctgaaat atcaatgccg atgcggccta cctgtgcgga tgcagcggga aggccgttag
     5821 tcagcagcgt ccaggtatcg cctccgtcgt aactccggta gataccgctg ccgggcccgc
     5881 catgtgggaa agggtgcgcc gtccgcacag gagggactat ggcggcgtaa agggtatcgg
     5941 gcttcgccgg atgcatgaca atatcgatag
//
LOCUS       DAOPNH010000214         5928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15485_length_5928_cov_5.490720, whole genome shotgun sequence.
ACCESSION   DAOPNH010000214 DAOPNH010000000
VERSION     DAOPNH010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15485_length_5928_cov_5.490720"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKZ74_10845"
     CDS             <1..244
                     /locus_tag="PKZ74_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93295.1"
                     /translation="LVTIKYVRTVKHEIMHFAAWLDDEGEFFDTVHFPPTLRTSPFKG
                     DGVYLIEGKVVEEFGFPSVEVERMEKMVLVADPRGG"
     gene            complement(412..1068)
                     /locus_tag="PKZ74_10850"
     CDS             complement(412..1068)
                     /locus_tag="PKZ74_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPM93296.1"
                     /translation="MNPQPVALIGATGLIGSHILDQLKNDTDIQSIRVLVRKPVEINH
                     PKVEVIRLDFADPGAYRKAIAGCQAVFCAIGTTNAKVKGDKTAYRKVDYDIPVNAARF
                     CAETGVQQFLLVSSVGANSQSNNFYLKLKGEVENEISRLSIPSIAIFRPSMLLGKRNE
                     SRPMESIAQAISKPLAFLFPSRYKPIAAADVARAMVAAGKQNHTGIRVYHYNEMITMV
                     "
     gene            1308..2168
                     /locus_tag="PKZ74_10855"
     CDS             1308..2168
                     /locus_tag="PKZ74_10855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPM93297.1"
                     /translation="MEIRTDIFLGDSKDVLREMEENSIDLIVTSPPYADQRSNTYGGI
                     KPDKYVEWFLPITEQLFRVLKPTGTFVLNIKEKVVEGERSTYVLELILEMRKQGWLWT
                     EEFIWHKKNSYPGKWPNRFRDSWERLLQFNKDRKFKMYQEAVMVPMGDWAKSRLKNLS
                     NTDKIRDNSKVGSGFGKNISNWLDREMAYPSNVLHLATECSNKSHSAAFPEELPEWFI
                     KLFTKEFDIVLDPFMGSGTSLVVAQRMKRHSIGIDIIPEYYEMVKSQFNRIEYILFEH
                     KPGYEQIKFK"
     gene            2146..2892
                     /locus_tag="PKZ74_10860"
     CDS             2146..2892
                     /locus_tag="PKZ74_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HPM93298.1"
                     /translation="MSKLNLNEVTQFVEKNIGSFHQSRIEKLNTIKLKEVIRHKNPYL
                     FKAKNVTTAQDIVKGILDAFLSSSEEGIFGNWLERLAIYINDQVYHGRKAGIEGIDLD
                     FDKGGNRYLVSIKSGPHWGNSSQVKKMIIEFDTARKRLATSGNKSIIVCVNGCCYGRS
                     NSKSEYKTSGNYFKICGQRFWELISNDPDLYVKIIEPLGFEAEKMNQEFSLLYSQVIN
                     RLTKEFLIDFCYDDGSINWEKLVAFNSGVA"
     gene            complement(2967..3950)
                     /locus_tag="PKZ74_10865"
     CDS             complement(2967..3950)
                     /locus_tag="PKZ74_10865"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM93299.1"
                     /translation="MKYFITGATGFIGGKLARRLVKEGHTINALVRDPAKATDLKELG
                     INVFKGDITDKSSLSEPMAGVDGVYHVAAWYKVGVKDKSMAYSINVNGTRNVLEVMKE
                     GGIKKGVYTSTLAVNSDTRGKIVNENYRYDGKHLSEYDRTKWLAHHEVALPMMKEGLP
                     LVVVMPGLVYGPGDTSAAGETFKRYLQGKLPVLPTQTAYNWAHVDDIVEGHILAMEKG
                     RAGEAYMIGGPVHELTEAMMMAEEITGVKAPRLHLSPAMMKASAGLMGFLEKFLPIPD
                     LFSGEMLRATAGVTYIGDSSKARRELGYDPRPLKEGLSETLGLLKLELGIK"
     gene            complement(4037..5065)
                     /gene="selD"
                     /locus_tag="PKZ74_10870"
     CDS             complement(4037..5065)
                     /gene="selD"
                     /locus_tag="PKZ74_10870"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPM93300.1"
                     /translation="MRLTQFTHGLGCACKLRPQVLEELLKNLPAEIFPDVLVGNEQAD
                     DAAVYRINDQQAIVQTLDFFTPIVDDPYTFGAIAAANALSDIYAMGARPLFALNIAGF
                     PSNRLPVSVLSQILKGASDKAAEAGISVIGGHTVDDTEPKFGMAVTGIVHPDKIWRNS
                     GAKPGDVLILTKPIGTGILATAMKQGMADQQSINRTIELMLDLNKTAAEVMLGFPVST
                     ATDITGFGLLGHLHEVISASRLDAEILVNAVPVIPGAEEMAAAGIIPGGTINNLEFSN
                     RFVKWDENISYVRRILLCDAQTSGGLLVSIPAGFADNIIKNLSDKGIKGVVIGRMSEK
                     GSGFIYVK"
     gene            complement(5216..5860)
                     /locus_tag="PKZ74_10875"
                     /pseudo
     CDS             complement(5216..5860)
                     /locus_tag="PKZ74_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
BASE COUNT         1610 a         1305 c         1427 g         1586 t
ORIGIN      
        1 cctggtcact atcaaatacg tacgaacggt gaagcacgag atcatgcact ttgctgcctg
       61 gcttgacgac gaaggggagt ttttcgacac cgttcacttc cctcccaccc tgagaaccag
      121 tccgttcaaa ggcgacgggg tttacctgat tgaaggaaag gtagtggaag agtttgggtt
      181 tccgagcgtg gaggtggagc gtatggagaa gatggtgctg gtggcggatc cgagaggcgg
      241 ttgagaaggt aggatggcag gacgatggga agatagaaga taggaagata ggactgtagg
      301 acggtaggaa aaagggccgt aaatgcagtt acccatattg cccgtagggc ataagctatt
      361 gtagaaatgg cagcgtaaaa gaccagatac cctgtagggg ttatatgaat atcagaccat
      421 cgtaatcatt tcattataat gataaacccg tattcccgtg tggttttgtt tgcccgcggc
      481 aaccatggcc ctggcaacat cggcagcggc gatgggcttg tatcgggatg ggaacaaaaa
      541 tgccagcggt ttggagattg cctgggctat cgattccatt ggccgggatt cgttgcgttt
      601 gcccagcagc atggatggcc ggaagatggc aatggagggg atggagaggc ggctgatctc
      661 gttttccacc tcacctttca gtttgaggta gaagttattg ctctggctgt tggctccgac
      721 cgaagagaca agcaggaatt gttgtacgcc ggtctcggca cagaaccggg cggcattcac
      781 agggatatcg taatcgactt tgcggtaggc ggttttgtcg cctttgacct tagcgttggt
      841 ggtgccgatg gcgcaaaaca cagcctggca accggcgatg gctttgcggt aagctcccgg
      901 gtcggcgaag tcgagccgga tcacttcaac tttcgggtga ttgatctcca ccggctttct
      961 gaccagcacc cggatggatt ggatgtctgt gtcgtttttc agttggtcca gaatgtggct
     1021 tccgatgagg ccggtagcgc cgatgagggc gacgggttga gggttcataa gtttaatata
     1081 gagaagagaa tcatatgaaa tgtttttata ggattgtagc tcaaagatag aaattaatca
     1141 gtcatgcgga aaatcaggaa ttactggtca attatcggca tagaatccaa gtgagttggt
     1201 agttgattaa tgtttaataa ggtcaatttg ataaaaatat tatcaattta caaacagcga
     1261 tgacttttag gttagcatga gttacttttg tgaatggaga ttgtattatg gaaatcagaa
     1321 cagatatttt tctaggtgat agcaaagacg tattaaggga aatggaagaa aattccattg
     1381 atctcattgt cacctcacct ccttacgccg atcagagatc aaatacctat ggaggaataa
     1441 aacctgataa atacgtcgaa tggtttttac ctattacaga gcaattattt cgagtactca
     1501 agccaaccgg aacattcgta ttaaatatta aggagaaggt tgttgaagga gagcgaagca
     1561 cctacgtgct tgagcttatt cttgaaatga gaaagcaagg atggctatgg actgaggaat
     1621 ttatctggca taaaaaaaac agttaccctg ggaaatggcc gaatcggttc cgggattcct
     1681 gggaaagact tcttcaattc aataaagata gaaagttcaa aatgtaccag gaagcagtaa
     1741 tggttccaat gggagattgg gcaaaatcga ggcttaaaaa tttatcaaac accgataaga
     1801 tccgtgataa ttcaaaagta ggcagtggct ttggcaaaaa tatttcgaat tggcttgatc
     1861 gtgaaatggc ttatccctct aatgtattac acctagctac tgaatgtagc aataaaagcc
     1921 atagtgccgc atttcctgaa gaactgccgg aatggtttat aaaactcttt acgaaagaat
     1981 ttgatattgt cttagatccc tttatgggat caggcacctc attggttgtt gctcaaagaa
     2041 tgaaaaggca ttcaattggt attgatatta ttcccgaata ttatgaaatg gtaaagtccc
     2101 aatttaatag aatcgaatac atattatttg aacacaaacc tggttatgag caaattaaat
     2161 ttaaatgagg taacccaatt cgtcgaaaag aatattggga gttttcacca gagccggata
     2221 gagaaattaa atactatcaa gctgaaggaa gtaattagac ataaaaatcc ttacctattt
     2281 aaagcaaaaa acgtaacgac tgcgcaggat atagtaaaag gaattcttga tgcattcctt
     2341 tcttcgagcg aagaaggaat atttggaaat tggctggaaa gactggccat ttatattaac
     2401 gatcaggtat accatggacg caaggcagga attgaaggga ttgatcttga ttttgataaa
     2461 ggcggaaata gatatttggt ttctattaaa tccggacccc attggggcaa ttccagccag
     2521 gtcaaaaaaa tgataattga gtttgataca gccagaaaac gtttagcaac ttcgggaaac
     2581 aaaagtataa tagtctgtgt taatggttgc tgctacggca gatcaaattc aaaatcagag
     2641 tataagacat cgggtaatta ttttaaaatc tgtggacagc gattttggga gcttatttcc
     2701 aatgacccgg atttatatgt aaaaataatt gagccactag gatttgaagc tgagaaaatg
     2761 aatcaagaat tttcactgct ttacagccag gtcattaaca ggcttacaaa agaatttttg
     2821 attgattttt gttatgatga tggaagtata aattgggaaa agctggtagc ttttaattcg
     2881 ggagtagcct aagaatagag agaggttatc actgcattgt tgtctcttgc aactattaaa
     2941 agtttttaaa ctagcatttc aatcttctat ttaattccca actcaagttt aagtaatcct
     3001 agcgtctcac tgagaccttc tttcagcgga cgcggatcgt atccgagctc gcggcgggct
     3061 ttgctgctat cgccgatata ggtgactccg gccgtagcac ggagcatctc gcccgaaaaa
     3121 agatccggga tgggaagaaa cttttccagg aaacccatta aaccggcgga tgctttcatc
     3181 attgccggcg acaaatgcaa ccgcggagct tttactccgg tgatttcttc cgccatcatc
     3241 attgcttctg tcagttcgtg gaccggcccg ccgatcatgt aggcttcgcc tgccctaccc
     3301 ttttccatcg cgagaatatg accttcaacg atatcatcga cgtgggccca gttgtaagcg
     3361 gtttgagtgg gcaggacagg caatttgccc tgcaggtatc ttttaaaggt ttcgccggcc
     3421 gcgctggtat cacccgggcc atataccaat ccgggcatga ccaccaccag cggcaatccc
     3481 tctttcatca taggcagtgc cacctcgtgg tgcgccagcc attttgtccg gtcatactcg
     3541 ctgagatgct taccatcgta cctgtagttc tcattaacga tcttcccgcg ggtatctgag
     3601 ttaaccgcca gggtgctcgt ataaacacct ttctttattc ctccttcctt catgacttca
     3661 aggacatttc gcgtaccgtt gacattgatg gaataagcca tactcttatc cttaacccca
     3721 actttatacc atgcagcaac atggtatacc ccatcgactc ctgccatggg ttcggaaaga
     3781 gatgatttat cggtaatatc acctttaaag acattgattc ccaattcttt caaatcggtg
     3841 gctttagcgg gatcacggac tagcgcattg atcgtatgtc cttctttcac aagtcttctg
     3901 gcaagctttc cgcctataaa tccggtagca ccggtaataa aatatttcat aatcagtttt
     3961 tctgaaaaat tctttaatcc tcgcggatca cacaggaaat aacacagcag gcgcagatat
     4021 gtttattccc gcaacgttat ttaacataaa taaagccact acccttctct gacattcttc
     4081 caatgaccac tcctttaatt cctttatccg atagattctt tataatatta tcggcaaacc
     4141 cggctggaat tgacacgagc aatcccccgc tggtctgggc atcacaaagg aggatccttc
     4201 ggacataact gatattctca tcccatttaa caaatctgtt cgagaattca aggttattga
     4261 tcgttccccc ggggatgatg ccggcagccg ccatttcttc cgctccggga atgaccggaa
     4321 cagcattgac caatatttca gcatccagtc gggaggctga gattacttca tgcaaatgcc
     4381 ccagcaatcc gaaaccggta atatcggttg cagtagaaac cggaaatccg agcataactt
     4441 cggctgctgt tttgttgaga tcaagcatta gttcaattgt tctattgatc gattgttggt
     4501 ctgccattcc ttgtttcatc gctgtagcca aaattcctgt tccgattggt tttgtaagga
     4561 taagaacatc accgggtttg gcgcctgaat tacgccatat tttatcggga tgaacgatgc
     4621 ccgtcacagc cattccgaat ttcggttcgg tatcatcgac ggtatgacct ccgataaccg
     4681 aaatacccgc ctcggctgct ttatcggatg caccttttag aatttgagag agtacactga
     4741 ccggaaggcg gttcgaaggg aaaccggcaa tattcagggc aaacagaggc cgggcgccca
     4801 tggcgtagat atcgctaagg gcattggctg ctgcgatggc accaaaagtg tacggatcgt
     4861 cgacaatggg tgtgaaaaaa tcgagcgttt gcacgatggc ctgttgatca ttgatacggt
     4921 aaacggcggc atcatcggcc tgctcgttgc caactaaaac atcaggaaaa atctcagccg
     4981 gaaggttttt cagcaattcc tccagaacct gcggccttaa tttgcatgca cagcccagcc
     5041 cgtgcgtaaa ttgagtcaac cggatctctt ttaaatcttc tccttcggcg gccagctgcc
     5101 tactgccaac tgccgactga cgactaacga cctggacaac agccgctgct gcgtgctcaa
     5161 catcctgttc actcagaaac ctgcctgtcg agaaccggat cgtgcccatg gcatattcaa
     5221 ccggtacatt catggctttc agcgtggccg acatgctggt ttcccctgca tgacaggctg
     5281 cgccggcaga ggcagctacc tcctccgcca actccgaaag gatggtattc gcttcaattc
     5341 ccggaaaaga aaggctaagc gtattcggca ggcggtattt cgggtgcccg tttaagcgta
     5401 ccatcgggat ttccatcctg atcttctcat aaagcatctc tcgtaacccg tgtgaatgct
     5461 ttgcattgac atccaggtcg cgcagggata tgtcacatgc ttctcccagg ccggcgatca
     5521 gggctacatt ttcggtgccc gcccggagat tctgttcatg gtcggctcca tggatcagtt
     5581 tttcgagctt cacccctgcc cttacgaata gcgccccgat ccctttggga gcataaagct
     5641 tatggcctgc aacagacagc agatcgacac ccagatcatc gatccgtatt ttgccggccg
     5701 actgggcggc gtcggtatgg aaaagaatac cgtgattatg ggcgatggcg gccaactccc
     5761 agatgggttg aatggtgccg gtttcattgt tggcatgcat cacggttacc aggattgttt
     5821 cgggtgttat ggccttttca agatcggttg ggcaaaccac tccataccct tcaaccggaa
     5881 gccagggaag cttaaagcct tgtttttcaa gataccggca gactgcgg
//
LOCUS       DAOPNH010000215         5925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15492_length_5925_cov_6.069847, whole genome shotgun sequence.
ACCESSION   DAOPNH010000215 DAOPNH010000000
VERSION     DAOPNH010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_5925_cov_6.069847"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PKZ74_10880"
     CDS             <1..1150
                     /locus_tag="PKZ74_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPM93301.1"
                     /translation="RIEARGNDYSLLHVIKPEIDLPPEIDHYAREVYDKALENFKKFQ
                     DKKWLVSDEKECLYIYAQTMNNKIQYGLVACASVEDYMNGIIKKHELTRPDKEEDRKK
                     HVRITNANMEPVFFTYPAHPEIDRIIATHVASFPPVYDFISVDGIGHHFWIIEGPETI
                     DQLIRLFDLIPFTYVADGHHRTAAAALVGNEKKLANPNHTGKEEYNYFLAVHFPDNQL
                     TIIDYNRVVKDLNGLNKEEFLGKISANFDLVLKGEDIYKPEHLHEFSMYLDGQWYSLE
                     AKPGTYNDHDPIGVLDVTILSNLVLDEILGIKDLRRDKRIDFVGGIRGLGELKRRVDS
                     GEMAVAFALYPVSMKQLIDIADSGNIMPPKTTWFEPKLRSGLVIHKLD"
     gene            1161..1337
                     /locus_tag="PKZ74_10885"
     CDS             1161..1337
                     /locus_tag="PKZ74_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPM93302.1"
                     /translation="MEIKEIVFKTLKDAREPLKSGEIAEKCGQDKAAVDKAIKALVKE
                     ERIDSPKRCFYQAK"
     gene            1369..2037
                     /locus_tag="PKZ74_10890"
     CDS             1369..2037
                     /locus_tag="PKZ74_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPM93303.1"
                     /translation="MTVRHNLEYLLKEIPAGVKLVAVSKTMPAEVILEAYNAGQLRFA
                     ENKSQELTSKQPALPQDIKWHFIGHLQTNKVKYIASFVDMIQSVDSLKLLKEIEKEAS
                     KNKRVIDCLLQFHIATEETKYGLDPDEAMAILNSPEYNECHHIAIRGVMGMASFSDDQ
                     TLVRREFRSLKNIFDRLKNDFFGTDPGFCEISMGMTGDYKIAIEEGSTIVRIGTAIFG
                     SRIL"
     gene            complement(2034..3194)
                     /locus_tag="PKZ74_10895"
     CDS             complement(2034..3194)
                     /locus_tag="PKZ74_10895"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPM93304.1"
                     /translation="MKEKIISAFTELYGEGNGVIRVYFAPGRVNLIGEHTDYNNGYVF
                     PCALTFGTYLAVRPTNRPFLEFYSHGFEYAAKIPFQSAYLNEGSEWINYPLGIIETMR
                     QEGKVTGGIQLYFRGDIPSAAGLSSSASIEMVTAFALNDVYKWKYTIPDLIRISKKAE
                     NEFIGVNCGIMDMFSIGQGKSGHAIFLDCGTLNFELVPANITGHRLVIINTNKKRGLG
                     DSKYNERVGECRKAVNYLSKSLNVNSLGEISLIDFRQNEHLITDAIIQKRARHIITEN
                     QRVLDAVKALKNNDLSTFGQLMNDSHFSLRDDYEVTGFELDSLVEIMLQQDGVVGARM
                     TGAGFGGCALAFVDEHHLEEFLGNVPVAYQNRTGYRAEVYMPEIGDGVKRIA"
     gene            3262..3335
                     /locus_tag="PKZ74_10900"
     tRNA            3262..3335
                     /locus_tag="PKZ74_10900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3296..3298,aa:Arg,seq:acg)
     gene            3400..3969
                     /locus_tag="PKZ74_10905"
     CDS             3400..3969
                     /locus_tag="PKZ74_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM93305.1"
                     /translation="MDPLIFKIIEEFYPPGSLRNRIYVPHCQAVTTLALKIAQSNPGL
                     NADEQYIQFGGMLHDIGIFYTETPDFGCYGRLPYLAHGYMGRELLEKKGLKDIAAVCE
                     RHIGVGITIEEIKQNNLPLPVRDMVPQTIEEKIICYADKFFSKSASNLSQPKPLDKVK
                     RSIRKYGDTKWDVFEEMMNLFGTESVYGK"
     gene            complement(3976..5619)
                     /locus_tag="PKZ74_10910"
     CDS             complement(3976..5619)
                     /locus_tag="PKZ74_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPM93306.1"
                     /translation="MMKLSSSLATLVIMVILNSCISKEKADLILFNGKIYTTDSAFSI
                     KEALAVSNGNFIATGTNEEILSAYSSEIIVDAGGKVILPGLIDGHCHFFGYAQNRYQG
                     VNLRGTSSFEEIVDKLKDFHSLYPEKWITGTGWDQNDWNDKAFPDNLILNTLFPDIPV
                     ILTRIDGHAVLANACALHLARIDSKTVIPGGKVLLKDGKPTGMLLDNAADSMKKLIPA
                     PDQQSRALALQSAQEDCFRYGLTSVVDAGLDYSEIKLIDSLQNSGHLKIRINAMLSPT
                     DKNIREFLGKGSFRKERLAVNSLKLYADGALGSRGALLLEPYADDPGNYGVMVADESY
                     YRELLSMAYNSGFQVNTHCIGDSANRMILNLYREVLNGPNDRRWRIEHAQVVNPGDFR
                     LFGDYRIIPSVQATHCTSDMYWAGERLGPDRIKYAYAYKKLLDENGWIINGTDFPIEE
                     VNPMLTFFASTGRMDKSGYPENGFQTENALSREETLRSMTIWAAKGSFEDDIKGSIEP
                     GKVADFVILDRDIMEVPFPEIPKTVVLKTYLNGELVFEN"
     gene            5737..>5925
                     /locus_tag="PKZ74_10915"
     CDS             5737..>5925
                     /locus_tag="PKZ74_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93307.1"
                     /translation="MSKLKLHSGLIFIISIIFTGALFRFIPHWPNFTPIAAMALFGGA
                     YLGRKHLAFLLPFAAMFLS"
BASE COUNT         1627 a         1362 c         1328 g         1608 t
ORIGIN      
        1 gcgcatcgaa gcccggggga atgattattc cctgttgcat gtgataaagc ccgagataga
       61 tctgccccct gaaatagacc attatgcccg ggaagtttac gacaaagccc tcgaaaattt
      121 taaaaaattc caggataaaa aatggcttgt ttcagatgaa aaggaatgcc tttatatcta
      181 tgcccaaacc atgaataaca agatccagta tggccttgtg gcctgcgctt cggtggaaga
      241 ttacatgaac gggatcatca agaagcatga actgacgcgg cccgataagg aagaagacag
      301 aaaaaagcat gtcaggatca ctaatgccaa tatggagccc gtcttcttta cctacccggc
      361 tcatccggag atcgacagga tcatagctac acatgttgct tctttcccgc ccgtttatga
      421 tttcatatcg gtagacggca tcggacatca tttctggatt attgagggtc cggaaacgat
      481 cgaccagctg atcaggttgt tcgacttgat cccattcacc tatgtggctg acggccacca
      541 ccggacggct gccgcagccc tggtaggcaa tgagaaaaaa ctggccaatc cgaatcacac
      601 gggaaaagaa gaatataatt atttcctggc cgttcatttt cctgataacc aattgacaat
      661 cattgattat aacagggttg tcaaagatct gaacggattg aataaagaag aatttctcgg
      721 taagatctcc gctaactttg acctggtgct gaaaggggaa gatatctata aacctgaaca
      781 tttgcatgaa ttcagcatgt acctcgatgg gcaatggtat tccttggaag cgaaaccagg
      841 aacctacaac gatcacgacc cgattggtgt cctggatgtg acgattctgt cgaatctggt
      901 cctggatgaa atcctgggaa ttaaagacct gcggagggac aagcggatag actttgtcgg
      961 gggtatccgt ggcctgggcg agctgaaacg ccgcgtcgac agtggagaga tggctgtggc
     1021 ttttgccctt tatcctgttt ctatgaaaca actgattgat attgccgaca gcgggaatat
     1081 aatgcctccc aaaactacct ggttcgaacc caaactgcgg agcggcctgg tgattcataa
     1141 acttgattaa ttctaatgaa atggaaatta aagaaatagt tttcaaaacg ctgaaagatg
     1201 ccagggaacc cctgaaaagt ggtgaaatcg ctgaaaagtg cggccaggat aaagcagccg
     1261 ttgataaagc catcaaagcg ctggtaaaag aagaacgtat cgattccccg aaacgctgtt
     1321 tttaccaggc aaagtgaaag cccgtaggat cctcattacc tggtgatgat gacagtcagg
     1381 cataacctgg aatacttact gaaagaaatc cctgccggcg ttaaattggt tgccgtttcg
     1441 aaaacgatgc cggcagaggt tatcctggaa gcatacaacg ccggacaact tcgttttgcg
     1501 gaaaacaaat cgcaggaact gacttcgaaa caaccggccc ttccacagga tatcaaatgg
     1561 cactttatcg gtcatcttca aaccaacaag gtgaaatata ttgcatcctt tgttgatatg
     1621 atacaatcag ttgattcact gaagcttctg aaggagatcg aaaaggaagc gagtaagaac
     1681 aaaagggtta tcgactgcct gctccagttt catattgcaa cggaagaaac caaatatgga
     1741 ctggatccgg atgaagcgat ggctatttta aatagtcctg aatacaacga atgtcaccat
     1801 attgcaatca ggggtgtgat gggaatggcc tctttctcag atgatcaaac actggtcagg
     1861 cgggagtttc gcagcctgaa aaatatcttt gaccggttaa agaacgattt ttttggaaca
     1921 gatccaggct tttgcgagat cagcatggga atgaccggcg attataagat cgctatcgag
     1981 gaaggcagca ccatcgtgag gataggcacg gcaatttttg gttccagaat actttaagct
     2041 attcttttca cgccatcccc aatttccggc atataaactt ccgcccggta acccgttctg
     2101 ttttgatatg caaccggaac gttcccaagg aattcttcca ggtgatgctc atcgacgaaa
     2161 gccagcgcac aaccgccaaa accggcgccg gtcatacggg cacccacaac cccatcctgc
     2221 tgtaacataa tttctaccag gctatccagc tcaaatccgg taacctcata gtcgtcacgg
     2281 agcgaaaaat gagaatcgtt catcaattgc ccgaaagtgc tgaggtcatt gttctttaat
     2341 gctttgacag catccagaac ccgttggttt tcggttatga tatgcctggc tcttttttgg
     2401 atgatggcgt cggtgatcaa atgttcattt tgacgaaaat caatgagact aatttctccc
     2461 aggctattga cattcagtga ctttgaaaga taattcaccg cctttcggca ctcccctacc
     2521 ctttcattgt attttgaatc acccagccct ctttttttat tggtattgat gatgacaagc
     2581 ctgtggccgg taatattcgc cggaaccagc tcgaaattta atgtgccgca gtcaaggaaa
     2641 atggcatgcc cggactttcc ctgcccgatt gaaaacatat ccataatccc acaattaact
     2701 ccgataaatt cattctcagc ttttttggat atgcggatca ggtcggggat cgtgtatttc
     2761 cacttataaa cgtcattcag ggcaaatgcg gtaaccattt cgatggatgc ggaagatgag
     2821 agccctgctg cggaaggaat atctccccgg aaatataact gaatccctcc ggttacctta
     2881 ccctcttgcc gcatagtctc gataatcccc aagggataat tgatccattc actgccttca
     2941 ttaaggtaag cactctgaaa tggaatcttt gcagcatatt cgaagccatg gctgtaaaat
     3001 tcgagaaatg gtctgtttgt cgggcgaaca gccaggtagg tgccaaaagt taatgcacag
     3061 ggaaaaacgt aaccattgtt ataatccgta tgttcgccga tcaggttgac ccgtccgggc
     3121 gcaaaatata ctcttataac gccatttcct tctccgtaaa gttctgtaaa agcggagata
     3181 atcttttctt tcacggtcat ctcaatataa ctgtcaaaaa tattaaaaga tttattactt
     3241 ttgcaccggt ttcacaccga tggacccgta gcttaattgg atagagcatc tgactacgga
     3301 tcagaaggtt gggggttcga ctcccttcgg gttcacacac ttggatttga gtcaggcagt
     3361 aggcaatttt tggttactgc cgtttttttt ctgtaacaga tggatccgct gatttttaag
     3421 atcatagaag aattctatcc gcccggttcg ctgaggaaca ggatttacgt gccacactgc
     3481 caggcggtaa caactctcgc tttaaaaatt gcccagtcca atccgggctt aaacgccgat
     3541 gaacaatata ttcagtttgg cggaatgctc catgatatag gcatttttta tactgaaaca
     3601 cccgattttg gctgctacgg ccgcctgccc tatcttgccc atggatacat gggaagggaa
     3661 ttgctggaga agaagggcct gaaagatatt gcagcggtat gcgaaaggca tatcggtgtc
     3721 gggatcacca ttgaagagat caaacaaaat aacttgccat tgcctgtcag ggatatggtt
     3781 cctcagacca tcgaggaaaa gatcatctgc tatgcagata aattcttctc aaaatcagca
     3841 agtaaccttt ctcaacctaa acctcttgat aaggttaaaa ggagcatcag gaagtacggc
     3901 gatacaaaat gggatgtatt tgaagagatg atgaatttgt tcgggaccga atctgtatac
     3961 ggaaaataat ttcggtcaat tttcaaagac cagctccccg ttaagataag ttttaagaac
     4021 cactgttttt ggaatttccg gaaacggcac ttccataata tccctgtcaa gaatcacgaa
     4081 atcagctact tttcccggct caatgctgcc tttgatatcg tcttcaaaac ttccctttgc
     4141 tgcccagatc gtcatacttc gtagcgtttc ttccctgctg agggcatttt ctgtctgaaa
     4201 accattttcc gggtaaccgg atttatccat tctacccgtg gatgcaaaaa atgtcagcat
     4261 cgggttgacc tcttcgatcg gaaaatcggt gccgttaatg atccatccgt tttcatccag
     4321 cagctttttg taggcatagg catattttat cctgtcaggc cccagcctct ctcctgccca
     4381 gtacatatcg ctggtgcaat gcgtggcctg cacggaagga atgatccggt aatcgccaaa
     4441 aagacggaaa tccccgggat tgacgacctg ggcgtgttca atgcgccagc gcctgtcgtt
     4501 cgggccattt agtacttccc ggtaaaggtt taaaatcatc cggttagctg aatccccgat
     4561 gcaatgcgta tttacctgga atccgctatt gtatgccatc gacaggagtt ccctgtaata
     4621 actttcatcg gctaccataa ccccgtaatt gccgggatca tctgcatagg gctcgagcaa
     4681 taatgcgccg cgcgatccca gcgcaccatc ggcatacaat ttcaacgaat taaccgctaa
     4741 tctctctttc cggaatgatc ctttaccgag aaattcacgg atatttttat ccgtagggct
     4801 tagcatggcg ttaatcctga ttttcaaatg tccgctgttt tgcagggaat ctatcagctt
     4861 tatttcactg taatccagcc ctgcatccac aaccgatgtg agaccatacc tgaaacaatc
     4921 ctcctgagcg ctttgcaaag ccagggcgcg cgactgttga tccggcgctg gaatgagttt
     4981 tttcattgaa tcggcagcat tatcgagcag catacctgtc ggtttaccat cttttagcaa
     5041 aacttttccg cccgggatta cagttttact gtcgattcgg gccagatgaa gagcacaggc
     5101 gttggccaga acggcatgac cgtcaatccg ggtcaggatg acgggaatat ccggaaaaag
     5161 agtgttgagt atcaaattat ccggaaaagc tttgtcattc cagtcgttct ggtcccaacc
     5221 ggttcctgtg atccattttt ccgggtacag ggaatgaaag tctttcagtt tatccactat
     5281 ttcttcgaaa gatgatgttc ctctgagatt tacgccctgg taacggttct gggcatatcc
     5341 gaagaaatgg cagtgaccgt cgatcagccc gggaagaatc accttccctc ctgcatctac
     5401 aattatttcc gaagaatagg ctgagaggat ttcttcattt gtcccggtag caatgaaatt
     5461 cccgttgctg actgccaggg cctcttttat gctgaatgcg gaatcggtcg tatatatttt
     5521 tccgttgaat agtataagat cggctttctc tttggatatg cagctgttaa gaatcaccat
     5581 aatgaccaga gttgcgaggc ttgaagataa tttcatcata tttaagtctt tcacgttgcg
     5641 aacaaaatta agaaaagcct gttaatataa atctcgattt gaccggagga gatggtaaat
     5701 ccttcttata tttgtagccg atttaaaact gggaacatga gcaaactgaa attacattct
     5761 ggcctgatat ttattatttc catcattttt accggtgcat tgtttcgttt tatccctcat
     5821 tggccgaact ttacacccat agctgctatg gcattgtttg gaggcgcata cctgggaagg
     5881 aaacatttag ccttccttct gccttttgcc gctatgtttc tcagc
//
LOCUS       DAOPNH010000216         5921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15501_length_5921_cov_6.387999, whole genome shotgun sequence.
ACCESSION   DAOPNH010000216 DAOPNH010000000
VERSION     DAOPNH010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15501_length_5921_cov_6.387999"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKZ74_10920"
     CDS             complement(<1..773)
                     /locus_tag="PKZ74_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93308.1"
                     /translation="MKKVFLIFILLLICIISSAQSRWIQQYLDEYNAPALELIISYDQ
                     GYLLSGWITSNFPPYTWLIKTDVNGEILWQKLIGDSYYSSIGVDNVCINANGDIYLGG
                     GAYNNSDANPVLIKLNPCGEKEWCREIVTTSNIDFFSDIVNTPDGGCATLIYKTFFPE
                     SINRSGILKFSSDGALLWQKYYESSDQGVANEDLSNIIITPDNGFLISGFCYYPDPEN
                     TDLYWLHPYYIKTDSLGNFEWETVIHRETGDIGGDAHMTL"
     gene            complement(781..2574)
                     /locus_tag="PKZ74_10925"
     CDS             complement(781..2574)
                     /locus_tag="PKZ74_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93309.1"
                     /translation="MDNARVVGNKFDIAGMTIPVEPYKYGLYIEYSTGYTIEENYFEE
                     KKSGIGSIGLYISNSGKDPNEVYKNDFKNLDYGIVAYGINRDEEGAGLCLKCNNFETC
                     TSDIHVIPENDGYGNWLQGREQGIASMQGTNSQGSNTSPAGNTFTKLDDVPEAFNYFN
                     HENCNKIYYFRHQNNNTNLKIVPEPTSSPDWFEPLGVSNTVFNSQEEACPSNLGGGSI
                     NFSLEKNLISFENELLVTYSDSIQALKDGGDTYTLNLNVYTSTPEESYQIRQQLLDES
                     PYLSDTVIKSAIIKENVLPNVMIRDVLVANPQAAKSSEILDKVIERIDPMPEEMMSDI
                     LNGRIIKGALEVLEDKFASHNTLKYKSLIKLESYYKLDTLDFHGSLDSLTNLWLNETD
                     PEILYKLVFLYFNNDDSANCLSTLNSIPQLNELSPEQLQDFEDFSSLIEILWTLKQCN
                     CELDSTIAEQLMLLVFRASKPGALARNTLVANGMLTYNEPIYLSDELKSGIVMPDKPF
                     KGLQSEDRLQIFPNPAKEFIIVSYNLKNLNGQSSILINSIDGKPCYSLDLNGEINQVV
                     VSLSTFSAGTYVIQLENKGKIIGSSTFVIAK"
     gene            complement(2639..2866)
                     /locus_tag="PKZ74_10930"
     CDS             complement(2639..2866)
                     /locus_tag="PKZ74_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93310.1"
                     /translation="MKPVIRFFIAIIVALFAFQANSQVNDTLTFSLNDVTFDTIGSYV
                     RPVLAGCHYTDVVGAPDMNHHQNSNPLPEHL"
     gene            3236..4090
                     /locus_tag="PKZ74_10935"
     CDS             3236..4090
                     /locus_tag="PKZ74_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPM93311.1"
                     /translation="MKQIIVAIDFSKTSLNALAYGIHIAGKAGADVQMVWVDNTTSEE
                     VVFEGFAHEERNEKVALLKELQEKYARTLKNGKLDFKTRKGKVYIELAQQAKSSGADL
                     IIAGTHGVSGFEEYWIGSNAYRIVTSVPCPVITLRHDCAVGDIKKIIIPIDSSQETRQ
                     KIPVAVNVASLFDSEIHILSLYSTPLKSVQKRVDNYARQVEEYCKERNIRFVTVTKES
                     ENITLTTIDYAEAVSADMIAIMTEQETTTANIFLGPYAQQMVNHSPIPVLSMRAKELV
                     SIANGLNK"
     gene            4087..4515
                     /locus_tag="PKZ74_10940"
     CDS             4087..4515
                     /locus_tag="PKZ74_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM93312.1"
                     /translation="MRFTLVIGLVLLLQPFLSAQDAGMGHVSVIRDERIDSVLVNYES
                     LRLKLMENPDTKLIPGYRIQIFFDSGLNSSDRARQTRDEFLLLFPDIPAYVSWKAPNY
                     RVRVGDYKSRLDAEKDLQLIMISYPNAWVTKDEINFPIMK"
     gene            4526..5374
                     /locus_tag="PKZ74_10945"
     CDS             4526..5374
                     /locus_tag="PKZ74_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPM93313.1"
                     /translation="MKKILVAIDFSECSINALEHAITIARKADAGIEMIFVIRPDSSR
                     EMFTEGPQTMSAMVKDKFDELVEKYQPLMGKNKLEYLIKEGKVYHEIVKEADKKEIFL
                     VMAGTHGSSGFEEFWMGSNANRIVSAIKKPVITIRGGVSINRHLEKIILPLDSTPETR
                     QKVPFTAYMARIFDAEVHVLRVYTSSVKAVIRKVDSYAEQVVKHLEEDNIKYVLESVE
                     ADNISDSTIEYALKVNANLISIMTEQETAAKNLLLGPYAQQMVNHSPIPVLSIHPKEL
                     LVALSR"
     gene            complement(5476..>5921)
                     /locus_tag="PKZ74_10950"
     CDS             complement(5476..>5921)
                     /locus_tag="PKZ74_10950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPM93314.1"
                     /translation="ISPRFTIAASFGNVHGVYKPGNVKLQPVILNNSQKYIEQKLNTG
                     PKPVNFVFHGGSGSSQAEIREAISYGVIKMNIDTDTQWAMWEGVHEYYKKYQGYLQGQ
                     IGNPEGDDKPNKKYYDPRKWLREGESAMIKRLKVAFEDLNCVDRN"
BASE COUNT         1673 a         1303 c         1316 g         1629 t
ORIGIN      
        1 agtgtcatat gagcatctcc gccgatatca ccggtttctc tatgtatgac ggtttcccat
       61 tcgaaattac caagtgaatc cgttttgatg taatagggat ggagccaata taagtcagta
      121 ttttccggat caggataata gcaaaaacct gatatgagaa aaccattatc aggagtaata
      181 ataatgtttg aaagatcttc atttgcaact ccctgatcag aactttcata atatttttgc
      241 catagcaaag caccatcaga tgaaaactta agtattcctg aacgattaat cgattcgggg
      301 aagaaggtct tataaattag tgtggcacat ccgccatcag gagtatttac aatgtcacta
      361 aaaaaatcga tgttgcttgt cgtgacaatt tctctgcacc attctttttc accacacgga
      421 tttaatttaa ttaaaacagg attggcatca ctattattgt atgcaccccc ccctaaataa
      481 atgtctccat tagcattgat acagacatta tcgactccaa ttgaagagta atatgaatcc
      541 ccaattaatt tttgccataa tatctctcca ttgacatcag ttttaattaa ccatgtgtag
      601 ggaggaaagt ttgaggttat ccatccggat aaaagataac cctggtcata agaaatgatt
      661 aactcaagcg ctggcgcatt gtactcatcg agatattgtt gaatccatct ggactgagcg
      721 cttgagataa tgcaaatcaa taataaaatg aatattagaa atactttttt cattatcaaa
      781 tcatttagca atgacgaatg ttgaagatcc aattattttg cctttattct ccagttgaat
      841 aacataagtt ccagctgaaa atgttgataa tgatacgact acttggttta tttcgccatt
      901 caaatctaaa ctatagcaag gctttccatc tatggaattt attaaaatgc ttgactggcc
      961 gtttaaattc ttcaagttat aagaaacgat gataaactct ttagccggat ttgggaatat
     1021 ttgcaatcta tcctcgcttt gcagaccttt aaaaggtttg tcaggcatca caatgcctga
     1081 tttcaattcg tcggatagat aaatgggttc gttataagta agcataccat ttgccaccaa
     1141 tgtgttacgt gctaaggcac ccggcttaga tgcccggaat acgagtaaca ttaattgctc
     1201 agcaatggtg gagtccaact cgcagttaca ctgttttaga gtccaaagga tctcaattaa
     1261 ggaagagaaa tcctcaaaat cctgtaactg ttctggcgat aattcattca attgtggaat
     1321 agaattcaag gtacttaggc aattggccga atcatcatta ttaaaataca ggaaaacaag
     1381 cttatataag atttccggat cagtttcatt tagccagagg tttgtcaaac tatccaacga
     1441 tccgtgaaag tcgagggtat ccagtttgta ataagattcc agcttaataa gtgatttata
     1501 ttttaaagta ttgtgcgatg caaatttatc ttcaagaacc tcaagcgctc ccttgataat
     1561 tcttccattt aaaatatcac tcatcatctc ttcaggcata ggatcgatac gttcaattac
     1621 tttgtccaag atttctgaag atttggcagc ctgaggattg gcaaccaata catctcttat
     1681 catgacattg ggcaggacat tttccttaat aatggcagat tttattactg tatctgataa
     1741 atagggggat tcatcaagta attgctgcct gatctgatag gactcttccg gggtgcttgt
     1801 ataaacatta agattaagcg tatatgtatc ccctccgtct tttaatgctt gaatactatc
     1861 agaataagta actaacagct cattttcaaa tgaaatcagg tttttttcca gactgaaatt
     1921 aatgctccct ccccccaaat ttgaggggca agcctcctct tgtgaattaa atactgtatt
     1981 tgataccccc aaaggttcaa accaatctgg agaagaagtt ggttccggaa caatttttaa
     2041 attggtatta ttgttttgat ggcgaaaata atatatctta ttgcaatttt catgattaaa
     2101 ataattgaaa gcctcgggaa catcatccaa tttcgtaaaa gtatttccgg caggagaagt
     2161 gtttgatccc tgtgagtttg ttccctgcat cgaggcgatt ccctgttcgc ggccctgcag
     2221 ccagtttccg tatccgtcgt tttcgggaat aacgtggata tcgcttgtgc aggtttcgaa
     2281 gttgttgcac ttcaggcaaa gcccggcgcc ctcttcatcg cggttaatgc cgtaagctac
     2341 aattccgtaa tccaggttct taaagtcgtt cttgtatact tcgttcgggt cttttcccga
     2401 gttgctgatg tacaggccaa tggatccgat accggatttc ttctcctcaa aatagttttc
     2461 ctcaatggta taacctgtcg aatattcgat ataaagtccg tatttgtatg gttccacggg
     2521 gatcgtcatt ccggcgatat caaatttgtt gcccaccaca cgggcgttat ccatttcacc
     2581 tataaagata ctatattgat tggaataaaa atcggaattc ggctgattct gttcatcgct
     2641 ataaatgctc tgggagagga ttcgaattct gatgatgatt catatccggc gctccgacga
     2701 catcagtata atgacatcct gccaaaaccg gacgcacata gctgccgatc gtatcaaacg
     2761 tcacatcatt aagtgaaaac gtcaatgtat cgttaacctg tgagtttgcc tgaaaggcaa
     2821 aaagcgcaac aatgatggca ataaagaacc tgatgactgg tttcataaat agttggatta
     2881 aaggttaata aatcggaatt gtaagatttg atagcgccat tccatttgga ttgattgatt
     2941 ggtaatgagt gaatgaaacg gattgaacaa atatagttcg atcagataag aaagtcaagg
     3001 gcgtttgata actttttggt ttgagactcc atctggtttt agtgagatgg catctcattt
     3061 cagcgagact ccatctcaaa ttcgagatgg catctcaatt ggaaggagac tccatctcac
     3121 atagggcaga tggagtctcc gtttcccgca caaattcacc gtttactttt taacagtagg
     3181 aaatttcatt ttaatttctt aattttactg aaaattgaaa ccaaactgcc gggacatgaa
     3241 acagatcatt gtagccatcg acttctccaa gacatcgctc aatgcccttg cttacggaat
     3301 ccatattgcc ggcaaagcgg gtgcggatgt gcaaatggtg tgggtcgaca ataccaccag
     3361 cgaagaggtg gtgttcgaag gttttgccca cgaagagaga aatgaaaaag tagccctcct
     3421 gaaagaactc caggaaaaat atgcccgaac actcaaaaac ggtaaactcg attttaagac
     3481 gcgtaaagga aaggtctata tcgaactggc ccagcaggcc aagagctccg gggccgacct
     3541 gatcatcgca ggtacccacg gcgtttcagg cttcgaagag tactggatcg gcagtaatgc
     3601 ctaccgcatc gtcaccagcg tcccctgccc cgttattaca ttacgtcatg actgtgccgt
     3661 cggggatatt aaaaagatca tcatcccgat cgacagctcc caggagacac ggcaaaaaat
     3721 cccggtggcg gtgaatgttg caagcctctt cgattcggag atccatatcc tttcactcta
     3781 ttccaccccg ctgaaatcgg tgcagaaaag ggtcgataac tatgcccggc aggtggaaga
     3841 atactgtaaa gagagaaata tccggttcgt caccgtgacg aaagagtcgg aaaatattac
     3901 cctgacgacg atcgattatg cggaggccgt ttcggccgac atgatcgcca ttatgaccga
     3961 acaggagacc acgacggcca atattttcct cgggccctat gcgcagcaga tggtgaacca
     4021 ttcgccgatc cccgtcctga gcatgcgtgc caaggaactg gtgtccattg ctaacggatt
     4081 gaacaaatga gattcaccct ggtcatcggc ctggttttat tgctgcagcc ctttctcagt
     4141 gcgcaggacg ccggaatggg ccatgtctcc gtgatccgcg atgaaaggat cgacagtgtg
     4201 ctggtcaatt atgaatcgct caggctaaag ctcatggaga acccggatac caagctgatc
     4261 ccgggttacc ggatccagat attctttgat tcgggcctga attccagcga cagggcaagg
     4321 caaacaaggg atgaattcct cttgctgttt cccgatattc cggcctatgt cagctggaaa
     4381 gcacccaatt accgggtgag ggtaggggat tataaatcgc ggctcgacgc cgagaaagac
     4441 cttcaactga tcatgatcag ctacccgaat gcctgggtta ccaaagacga aattaatttt
     4501 cccatcatga aataacatac gagctatgaa aaagattttg gttgccattg atttctccga
     4561 gtgctccatc aacgcgctgg aacacgcgat tacgattgcc agaaaagctg acgcaggcat
     4621 cgaaatgatt ttcgttatac ggcccgattc atcccgcgag atgttcaccg aaggccctca
     4681 gaccatgtcg gctatggtca aagacaagtt cgacgagctg gtggaaaagt accagccgct
     4741 aatgggaaaa aacaaactgg agtatctgat caaagaggga aaggtatatc atgagattgt
     4801 caaagaggcc gataaaaagg agatcttcct ggtgatggcc ggcactcatg gctcatcggg
     4861 attcgaggag ttctggatgg gcagcaatgc caaccggatc gtgagcgcga ttaaaaagcc
     4921 ggtgatcacc atccggggcg gcgtcagtat caaccggcac ctggaaaaga tcatcctgcc
     4981 gctcgacagt acgccggaga cgcgccaaaa ggttccgttt accgcctata tggcccggat
     5041 attcgacgcc gaggtccatg tgttgcgggt ctatacctct tctgtcaaag ccgttatccg
     5101 gaaagtcgac agctatgccg aacaggtggt caagcacctg gaagaggaca atataaaata
     5161 tgtgcttgaa tcggttgaag cagataatat ctccgattcg acgatcgaat atgcgctcaa
     5221 ggtgaacgct aacctcattt ctatcatgac cgagcaggag acggcagcaa agaacctgct
     5281 actggggcct tatgcgcagc agatggtcaa ccattccccg atccctgttt tgagcatcca
     5341 tccgaaggag ttgctggtgg cgctgagcag gtaatagaca gtaggcagtc agcagtcagc
     5401 agtcagcagt tgatatttaa caattgaaca attggacaat tgaacaattg aaccacctga
     5461 cttagctgcg gggaattaat ttctatccac acaattcaaa tcctcaaatg ccaccttcag
     5521 ccttttaatc atggccgatt cgccttcgcg aagccacttg cgcggatcgt agtatttctt
     5581 gttgggtttg tcgtcgcctt cgggatttcc gatctgcccc tgcaggtagc cctggtattt
     5641 cttgtaatat tcgtggaccc cttcccacat ggcccattgc gtgtcggtgt cgatattcat
     5701 cttgatcacg ccgtaggaga tcgcttcccg gatctcggcc tggcttgagc ccgagccgcc
     5761 gtggaagacg aaattcaccg gcttgggccc ggtgttcagc ttctgttcga tgtatttctg
     5821 ggagttgttg aggatgaccg gttggagctt cacgttaccc ggcttgtaca cgccgtggac
     5881 gtttccaaag gaggcggcta tcgtgaagcg ggggctgatc t
//
LOCUS       DAOPNH010000217         5912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15535_length_5912_cov_6.141882, whole genome shotgun sequence.
ACCESSION   DAOPNH010000217 DAOPNH010000000
VERSION     DAOPNH010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15535_length_5912_cov_6.141882"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PKZ74_10955"
     CDS             <1..1270
                     /locus_tag="PKZ74_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HPM93315.1"
                     /translation="FDHPEKVLKELKSYWHRDPKKQTLFDEFSENTLSNFYFPFGIAP
                     NFLINNKNYMVPMVIEESSVIAAASSSAKFWAERGGFHAEVVSSEKLGQVHFVWKGDP
                     EKLKQAMPALKQELLAGTKAITANMESRGGGILDVELINMNDRIEDYYQLKVSFETVD
                     SMGANFINSVLEEFARILKEFLVKSPVFNEDEKDCEIIMSILSNYTPDCLVRAWVECD
                     ISRMHLGENDMNEEKFVWKFQKAIEIAKVDKYRATTHNKGIFNGIDALALATGNDFRA
                     IEAGAHTYACRTGKYQSLTELTLDNGMFHYELKVPLAMGTVGGLTSLHPMARFSLEML
                     GKPSARELMIIAACAGLANNFGAVKSLVTKGIQVGHMKMHLFNILNFYAATQEEKLKA
                     VEYFKNKKVSFKSVTDFINNHRSQILGVKS"
     gene            1274..2194
                     /locus_tag="PKZ74_10960"
     CDS             1274..2194
                     /locus_tag="PKZ74_10960"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HPM93316.1"
                     /translation="MQTGQFYSNGKFLISGEYAVIHGAKALAVPLRFGQEMSVTEMDS
                     GGLIHWQTKVQGIDWFSAIFNRKRFEVTETSDPTTAGFIRQLLLEASSFQPELARGTS
                     SFFIENEIGFDINWGLGSSSSLISNISYWLDIDPYALYKELFRGSGYDLFCARTDHSI
                     VFQLKNGEPVVQTDVFNPPFQHHIHFVYSGRKQDSQSSVSNFLKEGRIDPGILHKISH
                     ITDRMTMASDVAEFMELMNEHETIISDLIGLPPVQRELFPGFDGAIKSLGAWGGDFIM
                     AVSPRDDKYVKDYFFSKNLPVIFAWHEIVR"
     gene            2178..3218
                     /locus_tag="PKZ74_10965"
     CDS             2178..3218
                     /locus_tag="PKZ74_10965"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93317.1"
                     /translation="MRSSDKTVGNIAWRSPSNIALVKYWGKRPVQIPANPSLSMTLGK
                     AYTEMNFSYRRADKGEKKLDFLFEGLKNEKFALKIATFLDSLSGNHPEISNFHIQVSS
                     FNTFPHSTGIASSASSMSALGLCLAEFLAIVEDREFSLREASYFSRLASGSACRSVYG
                     GYTVWGESPYVDGSHDEYAVPLPFEVHPVFRDIKDNILIVSSVEKKVSSRAGHSLMEG
                     HPYAEARFRLACQNIGILVSALSSGDIETFIRITESEALNLHALMMSSSSSYLLIEPN
                     TVNIVNEIRRFREETTIPVCFTLDAGPNVHLLYPAEYEENVKVWIGQVLVKYCENGRW
                     IDDGTGTGPIRL"
     gene            3245..4195
                     /locus_tag="PKZ74_10970"
     CDS             3245..4195
                     /locus_tag="PKZ74_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93318.1"
                     /translation="MKPEVFYAKIMLFGEYSVICDSMGLTIPYAHFTGSLRFINQEEY
                     TNYEMATESNRQLLNYLDHLKKLSREKEFKARMNLPKLESDIKDGLYFESNIPQGYGI
                     GSSGALVAAIYDQYSEGTQKHRKIYTSHEIPNLKETFSRMESFFHGISSGLDPLLCYI
                     RHPLLIRNKMQIETVGIPREKFGSDHAIFLIDTGELGKTGPLVNLFLERCKNDGFLNQ
                     VQNVFIPANNGCIETLLKGDINPFFGHLTELSAFQFLYLPEMIPDKMKSLWEAGLHRD
                     DFKLKLCGSGGGGFLLGISRDFRTTRKYLKQKGFEPITVF"
     gene            4205..5269
                     /gene="rlmN"
                     /locus_tag="PKZ74_10975"
     CDS             4205..5269
                     /gene="rlmN"
                     /locus_tag="PKZ74_10975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPM93319.1"
                     /translation="MNPRRNNIRTLSLTELETFFHSIGAEKFRARQVYEWLWKKAARS
                     FDQMTNISVETRAKLSESFTLDSITLNTTQKSEDGTIKMSFRLFDGHIIEGVLIPSGT
                     RMTACISSQVGCNLACKFCATGMIRYIRNLNFDEIFDQVAIIAETARHEYNLPLSHIV
                     YMGMGEPMLNYQNVLKSAERISAPDGLAMSPQRITVSTVGVAPMIRQLGNDKVKFHLA
                     LSLHSAINEKRNQIVPYNLRNSLGDLIEAVKYFHQQTRTRITLEYVMLKGFNDTMEDA
                     KALAEFCKNFPVKINLIQYNPVENVPYEDSGLERTEAFKAFLEKKNLVVNIRRSRGKD
                     IDAACGQLANKILKQNQENK"
     gene            5272..>5912
                     /locus_tag="PKZ74_10980"
     CDS             5272..>5912
                     /locus_tag="PKZ74_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM93320.1"
                     /translation="MDNSFSIRGLIVDVIGGRIFPGSVLVQDGRIQEIREHPVDETVY
                     ILPGLVDAHIHIESSMLIPSEFARLAVIHGTVATVSDPHEIANVLGIEGIKFMIENGK
                     KVPFKFCLGASSCVPATPFETAGASLGPKEVEELLRMDEIGYLSEMMNFPGVLNSDPE
                     VMQKLEIARKYGKPVDGHAPGLRGEDARNYISAGISTDHECFELDEALEKASMG"
BASE COUNT         1579 a         1422 c         1471 g         1440 t
ORIGIN      
        1 tttcgatcat cccgaaaagg tgttgaaaga gctaaaatca tactggcacc gggatccgaa
       61 aaaacaaacc ctgtttgatg agttcagtga aaataccctg tcaaactttt attttccttt
      121 cggaatagca ccaaatttcc tgatcaacaa caagaactat atggtcccga tggtcatcga
      181 ggaaagttcg gtgattgccg cagcatcgag cagcgctaaa ttctgggccg aaaggggtgg
      241 attccatgcg gaagtggtct cttcggaaaa actgggacag gttcactttg tctggaaagg
      301 tgatccggag aagctcaaac aggcgatgcc cgccctgaaa caggaacttc tggccgggac
      361 caaagccatc acggccaata tggagagcag gggaggagga atcctcgatg ttgagcttat
      421 caatatgaat gaccggatcg aagattatta ccagttgaaa gtttctttcg agaccgtcga
      481 ttcgatgggg gctaatttca tcaactccgt cctggaagaa tttgcccgta ttctcaagga
      541 atttcttgtt aaaagcccgg tttttaatga agatgagaag gattgcgaga tcattatgtc
      601 aatcctctcc aattatacac ccgattgcct ggtcagagcc tgggtagaat gcgatatcag
      661 ccggatgcac ctgggtgaaa acgatatgaa cgaggagaag ttcgtttgga agtttcagaa
      721 agccattgag attgccaaag tcgataaata cagggccact acccacaata aagggatatt
      781 caacgggatc gacgcactgg ccctggcgac cggtaacgac ttccgtgcga tcgaagctgg
      841 cgcccatacc tatgcctgcc ggaccggcaa ataccagagc ctgactgagc tgaccctcga
      901 caatggaatg tttcattacg aactcaaagt gccccttgcc atgggtaccg tcggagggct
      961 gacctcctta caccctatgg cacgcttctc tcttgagatg ctaggcaaac ccagcgcccg
     1021 cgaactgatg atcattgcag cctgtgccgg gcttgccaat aattttggcg ccgttaagtc
     1081 gcttgtcacc aagggaatac aggtcgggca tatgaaaatg cacctgttca atatcctgaa
     1141 tttctatgcc gctacacagg aggaaaaact gaaagctgtt gaatacttta aaaacaaaaa
     1201 ggtttctttc aagtcggtta ccgatttcat taataatcac aggtcacaga tcctgggtgt
     1261 aaaaagctga gccttgcaga cgggacaatt ttattccaat ggtaaattcc tgatttccgg
     1321 ggaatatgct gttattcacg gcgctaaagc actcgccgtc ccgctccgct tcggccagga
     1381 aatgtctgtt acagaaatgg attccggcgg gttgatccat tggcaaacaa aagttcaggg
     1441 aattgattgg ttttctgcaa ttttcaatag aaaaagattt gaggtaaccg aaacctccga
     1501 ccccaccact gccggcttta tccggcaatt gctcctggaa gcatcttcgt ttcagccgga
     1561 actggcccgt ggaacctcct cctttttcat agaaaacgag attggtttcg atattaactg
     1621 gggattagga agcagttcgt cgttaatctc caatatttcc tactggcttg atattgaccc
     1681 atatgccctg tataaagagc ttttcagggg ctcgggatac gatctgttct gcgcccggac
     1741 agatcattcc atcgttttcc agctgaaaaa cggggaacct gtggttcaga ccgatgtatt
     1801 caatccccct tttcagcatc atattcattt cgtctattca ggacgtaaac aggattctca
     1861 gtccagtgtc agtaattttc tgaaagaagg ccggattgat cccggtattc tccataaaat
     1921 cagtcatata acagatagga tgaccatggc gtcggatgta gctgaattca tggaattgat
     1981 gaatgaacat gagactatca tctccgatct gatcggtctg ccgccggttc aaagggaact
     2041 attccccggt ttcgatggcg caatcaagtc actcggggca tggggcggcg atttcatcat
     2101 ggctgtatcg cccagagacg acaagtacgt caaagactat ttcttcagta aaaatcttcc
     2161 tgttatcttc gcatggcatg agatcgtcag ataaaaccgt tggaaacatc gcctggagga
     2221 gcccgtcgaa tatcgccctc gtgaaatact ggggtaaaag gccggttcag ataccggcta
     2281 atccctcctt aagcatgacc ctgggtaaag cttatacgga aatgaatttt tcctaccgga
     2341 gagcggacaa aggtgaaaaa aagctggatt ttctttttga agggttgaag aatgaaaaat
     2401 ttgctttaaa gattgcaact ttccttgact cattaagcgg caaccatccg gaaatatcaa
     2461 atttccacat tcaagtttcc tctttcaaca ccttccccca ttcgaccgga attgcctctt
     2521 cagcctcgag catgagcgcc ctcggcctct gcctggctga attcctggcc atcgtcgaag
     2581 accgggaatt ttcgctccgg gaagcatcct atttctcccg cctggccagc ggcagtgcat
     2641 gccgctcagt ttacggcggt tacaccgtct ggggcgaaag cccctatgtc gacggctcac
     2701 acgatgaata tgccgttccg ctgccgtttg aagttcatcc ggttttcagg gacatcaagg
     2761 ataatatact gatcgtcagt agtgtggaga agaaggtttc gagccgcgcc ggccactcgc
     2821 tgatggaggg acatccctat gcagaggcac ggttcaggct ggcctgtcag aatatcggaa
     2881 ttcttgtatc ggcattatcg tcgggcgata tcgaaacatt tatccggatc accgaatcgg
     2941 aagccctcaa ccttcatgcg ttgatgatgt cgtcatcatc ttcctattta ttgattgaac
     3001 cgaatacggt taatattgtt aatgaaataa ggagattccg tgaagagacg accatccctg
     3061 tttgctttac tcttgatgcc ggaccgaatg tgcatctgct gtatcctgca gaatatgaag
     3121 agaatgtaaa agtatggatc ggtcaggtac tggttaaata ttgcgaaaac ggcagatgga
     3181 tcgatgatgg taccggtaca gggcccatca ggttataagg aaattaaatt aaaattaaag
     3241 ataattgaag ccggaagttt tttatgcgaa gatcatgctg ttcggtgagt acagcgtcat
     3301 ttgcgactcg atggggttga ccattccata tgcccatttc accggttcgc tcaggtttat
     3361 caaccaggaa gaatacacca attatgagat ggcgaccgaa tcgaaccggc aactgcttaa
     3421 ctacctggat catcttaaaa aattaagcag ggagaaggag ttcaaagccc ggatgaacct
     3481 gccgaaactg gagtcggaca tcaaagatgg cctttatttt gaatccaata ttccgcaagg
     3541 gtatggtatt ggcagctccg gggcactcgt agcagcaatc tatgaccagt attcggaagg
     3601 tactcaaaaa caccggaaaa tatataccag ccatgaaata ccgaacctga aggagacttt
     3661 ttccaggatg gaatctttct ttcacggcat cagctcaggc cttgatccgt tactttgcta
     3721 catcaggcac ccgctgctca tccggaataa aatgcagatc gaaacggtgg gcataccgcg
     3781 agagaaattc ggttccgacc acgccatttt cctgatcgat acgggtgagc ttggcaaaac
     3841 ggggccactg gtcaatctct tcctggagcg ttgtaaaaat gacggatttt tgaaccaggt
     3901 gcagaatgtt tttatcccgg ctaacaatgg ctgtattgaa actcttctca aaggcgacat
     3961 aaacccgttc ttcgggcatc tgacagaact ttccgctttc cagttcctgt atctgccgga
     4021 gatgatcccc gataaaatga aatccctctg ggaagccgga ttgcaccgcg acgatttcaa
     4081 gctgaaactg tgcggttcgg gtggcggggg attcctgctg gggatatcca gggatttcag
     4141 aaccacgaga aagtacctga agcaaaaagg ttttgaaccg attaccgtat tttagaaaaa
     4201 gaaaatgaat cccaggcgga acaatatcag gacgctgtcg ctgacagaac tggaaacctt
     4261 ctttcattcg atcggcgctg aaaaattccg ggcccggcag gtgtacgaat ggctctggaa
     4321 aaaagcggcc cggtcgttcg accagatgac caatatttcc gtcgaaaccc gggcaaagct
     4381 gagtgagagc ttcaccctgg attctatcac cctcaacaca acccagaaga gcgaagacgg
     4441 aacgatcaaa atgtcgttcc ggctgttcga cgggcatatc atcgaagggg tgctgatccc
     4501 ctccggaacg aggatgacag catgcatctc ctcgcaggta ggctgcaacc tggcttgcaa
     4561 gttttgcgcg accggtatga tccgctatat ccgcaacctg aatttcgatg aaattttcga
     4621 ccaggtggcc attattgccg aaaccgcccg ccacgaatac aatctcccgc tttcgcatat
     4681 cgtttatatg ggaatgggcg aacccatgct gaattaccag aacgtcctga aatcggcaga
     4741 gcggatttcg gcacctgacg gcctggcgat gtcgcctcaa cggatcaccg tatcaacggt
     4801 cggcgtggcg ccgatgatcc gtcagctcgg caacgacaaa gtgaagttcc acctggccct
     4861 gtcgctccac tctgccatca atgagaaacg caaccagatt gtcccttata atttgcgcaa
     4921 cagcctgggc gacctgatcg aggcggtgaa atatttccac cagcagaccc gtacccggat
     4981 cacactcgaa tatgtgatgc tgaaaggatt taacgatacc atggaggatg cgaaagcgct
     5041 ggctgagttc tgtaaaaatt tcccggtcaa gatcaacctg atccagtaca acccggtgga
     5101 gaatgtccct tacgaagatt ccggattgga gcgaacagaa gcgttcaagg ccttcctgga
     5161 gaagaaaaac ctggtcgtca atatccggcg gagccgcgga aaagatatcg atgctgcctg
     5221 cgggcaactg gccaataaaa ttctgaaaca aaaccaggaa aataaataac catggataat
     5281 tcattttcaa tccgcggcct tattgtcgat gtgattgggg gacggatctt ccccggcagc
     5341 gttctggttc aggacggtcg gattcaggaa atacgcgaac atcctgtcga cgaaacggta
     5401 tatattcttc cgggactggt cgatgcccat atccacatcg aaagttccat gctgatcccg
     5461 tcggaattcg cccggctggc tgtgatccat ggaacggtgg caacagtctc cgacccgcat
     5521 gagattgcta atgtgcttgg cattgagggg attaaattca tgattgagaa tgggaaaaaa
     5581 gttcccttta aattctgcct cggggcctct tcctgtgttc ctgccactcc gttcgagaca
     5641 gccggtgcct cgctggggcc aaaggaagtg gaggaactgc tccggatgga tgaaatcggt
     5701 tatctctcgg aaatgatgaa cttcccgggt gtcctgaaca gcgatcccga ggtaatgcaa
     5761 aagctggaga tagcccggaa atacggcaag ccggtcgatg gccatgcgcc ggggctcagg
     5821 ggggaagacg cccggaatta catctctgcc ggtatttcta ccgaccatga gtgttttgag
     5881 cttgatgaag cgctcgaaaa agcgtcgatg gg
//
LOCUS       DAOPNH010000218         5902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15577_length_5902_cov_6.331794, whole genome shotgun sequence.
ACCESSION   DAOPNH010000218 DAOPNH010000000
VERSION     DAOPNH010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15577_length_5902_cov_6.331794"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1984
                     /locus_tag="PKZ74_10985"
     CDS             80..1984
                     /locus_tag="PKZ74_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPM93321.1"
                     /translation="MIKYWGHSTFRPMQEEIIRSALDGKDTLALLPTGGGKSVCFQVP
                     ALAMQGVCLVITPLIALMKDQVENLKKKGIKAVAVYSGMHRNEIQMAFDNSVFGDMKF
                     LYLSPERLQTEQFQQALRRMKVCLLAVDEAHCISQWGYDFRPPYLKIADIRQFLPGVP
                     VLALTATATPGVISDIQKNLAFRAENVFRKSFERKNLTYAVIREEDKNSRLLRILKKV
                     PGVGIIYVRNRKRTVETATFLRKQGIKADFYHAGLDMAARSRKQEAWIREDIRIICAT
                     NAFGMGIDKPNVRLVVHIDLPDSIEAYFQEAGRGGRDEKRSYAVTLYNAADIAKLKEN
                     LEKEYPPVEMIKAVYQSLGNYFQLPVGSGKDESFDFELFAFCRNYGYNPVVTYNALRF
                     LEREGYIAMNESLETPSRIHFEADRESIYRYQIENQSMDAFVKLLLRSYSGVFTEFVQ
                     VNEVELARRAGLKEENVVANLVKLQKAGILSYAQRPARPRIIFPVERLDQKHLQISQV
                     NYHDRKKYALIRMESMIEYMTSEDQCRSQMLLRYFGEKNAPRCGLCDICLQRNKLNLN
                     EMEFNMVRDHVRTKLQARPMTLAELVFESGGHSEKHLLLVLRWLEDKGAVVRDEQMRY
                     SWRKQFKLGI"
     gene            2016..2603
                     /locus_tag="PKZ74_10990"
     CDS             2016..2603
                     /locus_tag="PKZ74_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPM93322.1"
                     /translation="MKLPLSFYRRPDVVQIAKDLIGKYIFTRIDGEVTSGIIVETEAY
                     EGATDRASHAYGNRRTARTEVMFAEGGVAYVYLCYGVHSLFNIVTNRKGIPHAILLRG
                     IFPVGGIEVMKRRAMKTKIGIASGIGPGNASKLLGIHYSLSGTDLMGGKIWLEDRGLD
                     LSAEIITGQRIGIAYAGPDALLPYRFYVRPEKIRA"
     gene            complement(2657..2929)
                     /locus_tag="PKZ74_10995"
     CDS             complement(2657..2929)
                     /locus_tag="PKZ74_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM93323.1"
                     /translation="MNKAELIDAMAKETGMTKADSKKALEAFVGSTMKALKKGDRVAL
                     VGFGSFSVAKRASRKGRNPQTGKEIKIAAKKVVRFKAGADLSNTVK"
     gene            complement(3109..4077)
                     /gene="fmt"
                     /locus_tag="PKZ74_11000"
     CDS             complement(3109..4077)
                     /gene="fmt"
                     /locus_tag="PKZ74_11000"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPM93324.1"
                     /translation="MQEAIKIVFLGTPEFAVDSLASLVDSGLQIAAVVTAPDQPAGRG
                     QKLRPSPVKEFALQNGLKVLQPVKLRDPEFINDLKALDADLFIVVAFRMLPGVVWQMP
                     RLGTFNLHASLLPQYRGAAPINHAIINGETETGLTTFLLKHEIDTGDILFREKIAIGP
                     DETAGELHDRMKVTGAGLVLKTAHALISGDYKLTDQSQLHRETEPLKSAPKIFRDFCR
                     IDWSGRSTEIFNKIRGLSPYPAAFTSLIPPEGEPVTVKIFRTGIGRDVPGTYPGQLTT
                     DNRSYLHVATSDGTIKILELQQSGKKMMKTDEFLRGFRINSDWRMG"
     gene            complement(4108..4791)
                     /locus_tag="PKZ74_11005"
     CDS             complement(4108..4791)
                     /locus_tag="PKZ74_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93325.1"
                     /translation="MAKKQDKTEGGIVAVEEALSKTERFIEKNQKILTIVIGAIVVVV
                     LVFFAFKRFYMAPREQESLEQMFMAERYFEGDSLSLALNGDGMYPGFLDIIDDYGMTK
                     GANLSKYYAGVCYLRMGNYEEALDYLKSFKGKDHILGPMAKGAAGDAYMELNQAAKAV
                     TYYLDAAESSKNEFTAPLFLMKAGWAYEVQNDYNKALKTYERIKQEFPTSNEGREIDK
                     YIARAKGKI"
     gene            4910..5092
                     /locus_tag="PKZ74_11010"
     CDS             4910..5092
                     /locus_tag="PKZ74_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPM93326.1"
                     /translation="MGKGDKKSKRGKIIMGSYGVRRPQRKTAAIAAPAPEPVATKPAK
                     KKSSPKSAKKENKAVE"
     gene            complement(5130..>5902)
                     /locus_tag="PKZ74_11015"
     CDS             complement(5130..>5902)
                     /locus_tag="PKZ74_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93327.1"
                     /translation="GAKMRILTEQKAGDIFIYNADIPGIKGRVASYEPVRRFLAFSAG
                     HRSGQGCYLQDNGIYMCNEKGNTLFAEITGDDYFLKGGHNRMNMMAAILAAIAAGAGS
                     EAIRAGLKTFKGLEHRLEYAGTYRDILFYNDSIATIPEAAIAAVKALPDTDTLILGGF
                     DRGLDYSGLVDFMVRSDVRNFIFLGKAGERIYKGFQTIENNKNLFYVASFEEAFQIIF
                     RVTNAGKICLLSPAAASYDIFRNFEERGKIFKKIARSY"
BASE COUNT         1526 a         1439 c         1449 g         1488 t
ORIGIN      
        1 tttggtggct acaaaaggat ttttagaaag ggctcaacaa ttataaaaca aaaaggatct
       61 gaccgaaatc cgcaacatat tgataaaata ctggggccat tccacattcc ggccgatgca
      121 ggaggagatc atccggtcgg cactggacgg aaaggatacc ctcgctttgt tgcccacggg
      181 cgggggcaaa tcggtctgtt tccaggttcc ggcgctggcc atgcaagggg tctgcctggt
      241 tataacccca ttaattgccc tgatgaagga ccaggtggaa aacctgaaaa agaaagggat
      301 aaaagcagtg gcggtctatt ccggtatgca ccgtaacgag atccagatgg cattcgataa
      361 cagcgttttc ggggatatga aattcctcta cctctcgccg gagcggttgc agaccgagca
      421 gtttcagcag gccctgcgcc ggatgaaagt ctgcctcctg gctgtcgatg aggctcactg
      481 catctcccag tggggatacg atttccggcc accgtacctt aagatcgccg atatcaggca
      541 gtttttgccc ggagtacctg tcctcgccct gacggccact gctactcccg gcgtgatcag
      601 cgatattcag aagaacctgg ccttccgggc tgaaaatgtc ttccggaaga gcttcgaacg
      661 gaaaaacctg acctatgccg tcatccggga ggaggacaag aacagccgcc tgttgcgcat
      721 cctcaaaaag gtcccgggtg taggtattat ctatgtcagg aaccgtaaac ggacggttga
      781 aacggccacc ttcctgcgca agcaggggat caaagccgat ttttaccacg ccgggctcga
      841 tatggcagcc aggagccgga agcaggaggc ctggatcagg gaggatatcc ggatcatatg
      901 cgctaccaac gctttcggga tggggatcga caagccgaat gtcaggctgg tggtccatat
      961 cgacctgccc gacagcatcg aagcctactt ccaggaagcg ggaaggggag ggcgcgatga
     1021 gaaaagatcc tatgccgtta cattgtataa tgcggccgat atcgccaagc tgaaagaaaa
     1081 cctcgaaaaa gagtatcctc ccgtcgaaat gataaaagcc gtctaccagt cgctcggtaa
     1141 ctatttccag ttgcctgtcg gaagcggcaa agatgagagt tttgattttg agctgtttgc
     1201 tttttgcagg aattacggtt ataacccggt tgttacatat aatgcactcc ggttcctgga
     1261 aagagaagga tatatcgcga tgaatgaatc gctggaaact ccttcgcgga tccattttga
     1321 agccgaccgg gaatcgatct accgctacca gatcgagaac cagtcgatgg atgcttttgt
     1381 caaactgctg ctaagatcct attccggggt ctttaccgag tttgtccagg taaatgaggt
     1441 ggagctggcc aggagagcag gattgaaaga ggaaaatgta gtggcgaacc tggtaaaact
     1501 gcaaaaagcc ggcatacttt cctatgcaca gcgcccggcc cggccccgca ttatttttcc
     1561 ggtggaaagg ctggatcaga aacatcttca gatttcccag gtcaattatc atgaccggaa
     1621 gaaatatgca ttgatccgta tggaatcgat gattgaatac atgacatcgg aagatcaatg
     1681 cagaagccag atgcttttac gatatttcgg tgaaaagaat gccccccggt gcggattgtg
     1741 tgatatctgt ctgcaaagga ataagctgaa cctgaatgag atggaattca acatggtgcg
     1801 cgatcacgtc cggacaaagc tgcaggcccg gccgatgacc ctggctgagc tggtatttga
     1861 atcgggtggc cacagcgaga aacacctttt gctggtactg cgctggctcg aagacaaagg
     1921 cgccgtggtc cgcgacgaac agatgcgcta cagctggcgc aagcaattca aacttggaat
     1981 ttagtggaac cctggaaccc tgcaaccctg gaactatgaa actccctctc tccttctacc
     2041 gtcgcccgga tgtagtgcag attgcaaaag atctcatcgg gaaatatatt tttacccgga
     2101 ttgatggcga ggtaacatcc ggtatcattg tcgaaacgga agcatatgaa ggggcaaccg
     2161 accgggcttc gcacgcctat ggcaaccgga ggacagcccg gacagaggtc atgtttgccg
     2221 aaggcggagt ggcttatgtc tatctttgtt atggtgtcca ctccttattt aatatcgtta
     2281 caaatagaaa aggcatccct cacgccatat tgctaagggg aatattccct gtcgggggaa
     2341 ttgaggtgat gaagcggagg gcaatgaaaa cgaaaatcgg cattgcctcg gggatcggcc
     2401 cgggcaatgc gtccaaattg ctcgggattc attattcgtt atccggtacg gatctgatgg
     2461 gcgggaagat ctggctggag gaccggggac tggatttatc ggcagaaata attacaggac
     2521 agcggattgg cattgcatac gccggccccg acgcgctgct tccataccgt ttttacgtac
     2581 gaccggaaaa gatcagggca taaaaaaacc ggcctcgcat aagcgacgcc ggttcttcag
     2641 ctttcgaaaa gaaggcttat ttcaccgtgt tggaaaggtc ggcgccggct ttgaaccgga
     2701 cgactttctt ggcagcgatc ttgatttctt ttcctgtctg cggattcctg cctttacggg
     2761 atgccctttt ggcaacggag aatgatccaa atccaactaa tgcaaccctg tcaccttttt
     2821 taagggcttt catggtgctg ccaacaaatg cttcgagagc tttcttggaa tcggctttgg
     2881 tcatgccggt ttcttttgcc atcgcatcga ttaattctgc tttgttcatg gttgtaagtt
     2941 ttttttaagg ttaacattac ataaaacact gcaaaattaa ggcattcaca aaaaaagtca
     3001 agcgaaaagc ccgtaattac ttgaaaatgt taataaatgg gtattattgt taataactta
     3061 agctgtaaaa gcgttgttag agtaaggttt taagactttt atcgactatt accccatcct
     3121 ccagtcagaa tttatccgga atccccttaa gaattcatcg gttttcatca tcttttttcc
     3181 tgactgttgc aactccagaa tttttatagt accatctgaa gtggctacat gcaagtaact
     3241 tctgttgtcg gtagtcaact gacccggata agttcccgga acatcccggc cgatcccggt
     3301 cctgaaaatc ttaacagtaa ccggttcgcc ttccggtggg atcagggatg taaatgctgc
     3361 agggtaagga ctgagccccc tgattttgtt gaaaatctct gtcgaccttc ccgaccagtc
     3421 gatacggcag aaatcgcgga aaattttcgg cgcgcttttc aggggttctg tttctctgtg
     3481 taattgcgat tggtctgtta gcttgtaatc gcctgaaata agggcatggg cagttttgag
     3541 caccaggcct gctccggtaa ccttcatccg gtcgtgcagc tcgccggctg tttcgtccgg
     3601 cccgatggct attttctccc ggaacaggat atcaccggta tcaatctcgt gtttaagtaa
     3661 aaaggtagtc agcccggtct ccgtttcacc gttgatgatc gcgtggttga tcggcgcggc
     3721 gccacggtac tggggcaaaa gcgaagcatg caggttgaag gttcccaggc gcggcatttg
     3781 ccagaccacc ccgggtaaca tgcggaatgc caccacaatg aaaagatcgg catccagtgc
     3841 tttcaggtca ttgataaatt cgggatccct caactttaca ggctgcaata ctttcaaccc
     3901 attttgaagg gcaaattcct taacgggaga gggcctgagt ttctggccgc gcccggcagg
     3961 ctggtcaggc gccgttacga ccgccgctat ttgcaagccg gaatccacaa gacttgccag
     4021 cgaatccacc gcaaattcag gggtacccag aaaaacgatt ttaatagctt cctgcatcag
     4081 aagccgaaat tacgaaaagt aaacggatta tatcttcccc ttggcccggg cgatgtattt
     4141 atctatttcc ctgccttcat tgctcgtcgg aaattcctgc ttgatcctct cgtacgtctt
     4201 gagggctttg ttataatcat tctgcacttc ataggcccag ccggctttca tcaggaaaag
     4261 cggcgcggtg aattcgtttt tggatgattc tgccgcatcg agataatagg taacggcttt
     4321 ggcagcctgg ttcagctcca tatatgcatc gccggccgca ccttttgcca tcggccccag
     4381 aatatgatct tttcctttga atgatttcag gtaatccagg gcttcctcat agtttcccat
     4441 cctcaggtaa cacaccccgg cgtaatattt ggaaagattg gcgcctttgg tcatgccata
     4501 atcgtcgata atatccagga aaccgggata catcccatcg ccattcaggg ccaggcttaa
     4561 cgaatcaccc tcgaaatagc gctctgccat gaacatctgc tccagcgatt cctgctccct
     4621 cggagccata taaaaacgct tgaaggcaaa gaataccagg actacaacta cgatggcccc
     4681 aatgacgatc gtcaggatct tctggttttt ctcaataaat cgttccgttt tacttaaagc
     4741 ttcttctact gcaactattc caccttcggt tttatcctgc tttttagcca taataggtta
     4801 cctttttaga tttcagggtg caaaagtaaa tctttttctt gatttggcaa gccataatgt
     4861 ttttattaac tttgttggtc aatcatataa tcatttaata ataaagatta tgggaaaagg
     4921 tgataaaaaa tcaaaacgcg gcaagatcat catgggatca tatggcgtca gaagaccaca
     4981 aagaaaaacg gccgcaatag ctgccccggc cccggaacct gtggcgacaa aaccggcaaa
     5041 aaagaaatcc tccccgaaat cggctaaaaa agaaaataaa gccgtggaat aatttcattc
     5101 atcttgttaa taaacagaat agcaaggagt taataacttc ttgctatttt tttgaatatc
     5161 tttccccgtt cctcaaaatt tctgaaaata tcgtaagagg cggctgccgg tgaaagaagg
     5221 catattttac ctgcatttgt tacccggaag atgatctgaa aggcctcttc aaatgatgcg
     5281 acataaaaca ggttcttgtt attttcaatc gtctgaaaac ccttgtaaat tctttcgcct
     5341 gcttttccca ggaaaatgaa atttctgaca tccgaccgga ccatgaaatc cacgagacct
     5401 gaataatcaa gcccccggtc aaacccgccg aggatcaggg tgtcggtgtc gggcaaggct
     5461 ttcaccgccg caatggccgc ctcgggtatc gtggcgatcg aatcgttgta aaacaggata
     5521 tcccggtagg tcccggcata ttccaggcgg tgttccaggc ctttgaaggt cttcagcccc
     5581 gcccggatgg cttcgctgcc ggccccggca gcgattgctg ccagtatggc agccatcatg
     5641 ttcatccggt tatggcctcc ttttaaaaaa taatcatcac cggtgatctc cgcaaaaagg
     5701 gtgtttcctt tttcattaca catataaatc ccgttatctt gcagataaca gccctgaccg
     5761 gaacgatgac ccgcagaaaa cgccaggaaa cggcgaaccg gttcatagga tgcaacacgc
     5821 cctttaatcc cgggaatatc agcgttatag atgaaaatgt cgcctgcctt ttgctctgta
     5881 aggatcctca ttttagcccc gt
//
LOCUS       DAOPNH010000219         5896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15596_length_5896_cov_5.240712, whole genome shotgun sequence.
ACCESSION   DAOPNH010000219 DAOPNH010000000
VERSION     DAOPNH010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15596_length_5896_cov_5.240712"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(742..2235)
                     /locus_tag="PKZ74_11020"
     CDS             complement(742..2235)
                     /locus_tag="PKZ74_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93328.1"
                     /translation="MKKLWMISALILAAATTTLSQTSTEAFRYSRTDIGGTARYMGLS
                     GAFGALGADFTTAGTNPAGLGLFKTSEFSITPAVHTGSVASSFNGTSGSDMRTNFYLG
                     NLGLVLTSKVQSKPNRPGWKYVTFATGLNRLNDFNYRYEMTGTNFTNSLLDVYAQDAD
                     KIKYTEIEQDPDGSFAYDLNLAWWDWLLDLKPGDPDSSSYVTPIPTGVDKLQTKTINS
                     RGSMNEYVFSIGSNYNNRLYLGLTLGIPSIRYFESTVYTESDIQSSDLKNFRRYEELE
                     TRGSGFNLKVGAIYRASDWLRVGAAFHTPTWYGNMRDYWQVTMTSEFNTPDLDGETIY
                     SRTSPSGYYEYSLQTPYRVQGNLAFIIGNVGLVSADYELADYSGARFDAYDYSFNGEN
                     SSIRNSYALTHHVRIGTEWRYNIFSFRAGAKYFTSPYQNDLNDGSRLGFSGGLGLKTE
                     WFFMDLAYAYNSMKDDYYFYNTEGISSGAVANTTVNHNILLTLGVKL"
     gene            complement(2255..3763)
                     /locus_tag="PKZ74_11025"
     CDS             complement(2255..3763)
                     /locus_tag="PKZ74_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93329.1"
                     /translation="MKNLAVLTVLAALFLASCSSTYNASAPYDEVYATSDKPSNTVSN
                     SKDVTVQSEPVESTTAYEGDYYSPGYEEGEFSSDEYYDYEYSSRIKRFHSDNPGFDYY
                     NNYYTDNYYYSYDPYYMGSSIYSGYGCCGPSFSLSFGPSWGYYGYPYYHYRPYYSYWD
                     PWYNPYYYGWGYGYPGYWGGSYWAGYNNGYWDGYWDGYYGGYYPIYPEPTPDGGYYGH
                     RGSRTGSTDGSNRTGLADRGSDRTGNDLKSSDPAGGDAKRDPNTSAIPSGTATRSEVT
                     TGGGNAVRTDVTPSTGGTATRNEVNPDGGNATRKEVQPATRTETPAEKPVYNKPAVER
                     PASTGGTATRTEGTSVSPSRRVEPEVKYEKPKTYTPPAARTTPSKQEYSSPSTRDSRT
                     YSGGQDGESTRKVYTTTPERKVNPVNTNPSQNYSTPARTNSRSTYTSPSGTQPRSYST
                     PAKSNSSPSYNSGTSTRSSSGSYSGGSSTRSSGSSSGSSSGSSSGSSRTGRR"
     gene            3876..5348
                     /gene="proS"
                     /locus_tag="PKZ74_11030"
     CDS             3876..5348
                     /gene="proS"
                     /locus_tag="PKZ74_11030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPM93330.1"
                     /translation="MAKDFSTREDNYAQWYNDLVIKADLAENSAVRGCMIIKPYGYAI
                     WEMMQSSLDKMFKDTGHVNAYFPLFIPKSFFSKEASHVEGFAKECAVVTHYRLKNSPD
                     GKGVVVDPEAKLEEELIIRPTSETIIWDTYRNWIQSYRDLPILVNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATEAEAVEEALKILDVYTRFAEEFLAMPVLRGVKSPNERFA
                     GAVETYCIEGLMQDGKALQAGTSHFLGQNFARAFDVKFLSRENKLEHVWATSWGVSTR
                     LIGALIMTHSDDHGLVLPPKLAPHQVVIVPIYKTAEQLAMITEKANSIKKDLESKGIR
                     VKYDGRDTQKPGWKFNEYEFKGVPVRIAIGPRDLENNTVEVARRDTLEKETLQADHLG
                     TKIEHLLQQIQKNLYQKALNYRENNTFKVDSWTEFKEKIEEGGFLLAHWDGTTETEEK
                     IKDETKATIRTIPLDGPKESGKCVYSGKPSAQRVVFARAY"
     gene            5611..>5896
                     /locus_tag="PKZ74_11035"
     CDS             5611..>5896
                     /locus_tag="PKZ74_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93331.1"
                     /translation="MKYLVLILALIIPSGILAQEFAPTGAIWHNSQSDWINPYLITYK
                     TIESVSDTIINGKSCKVLIEVERSYSDTAATFTHYMYSENDSVFFYKDNAF"
BASE COUNT         1420 a         1436 c         1614 g         1426 t
ORIGIN      
        1 gccggaacct ggagaacagg cacaatattt taccggtttc tgctcttcca accggaatgt
       61 acttttaccg gatcgttaat gaaaagaatg aggctattag cgtaggaaag tggattaaac
      121 tttagctaat atatacttta aaagcctaaa gtaccaaagg cacaaagggc ttcaagtcat
      181 aggcttatta ttagaattaa ctgctttgtt atattataag tattcatttg attaagaaaa
      241 taagaaggtt ggaaacccat ttacagttgg acagttgggc agttggacag ttgggcagtt
      301 ggacagttgg acaatgccat tacgcgattc ggcattcggc atggcgcatt ctcgatttag
      361 tttggtagtc cgacgggtaa atccccaaat ccccatattt ctcccgccag ggagaacagt
      421 atggtagcca gggaataaaa ggcgcaattc cgattatgag ttccgtcagg aacgacacgg
      481 atttaattga aatattatgg aaaatgtttc cgcgagctgg cggaccgaat cggttaattc
      541 tccgagactc catctgcccg atgtgagatg gagtctcatt gacaaataat ctaatatata
      601 attggaaccg acggattcac ccgtcagact gcaaacttat tgcggattcc cccgtcagac
      661 aaaaaaaaga ggctgctcaa actattgatg caacctcatg atttattcgt tcgcaatccc
      721 tttaagcaat tttaaagata cctacaactt cacccccagc gtcagcaata tgttgtggtt
      781 aacggtggtg ttagccacgg cgccggaact gatcccttcc gtattataga aataataatc
      841 atccttcatg ctgttgtaag cgtaggccag gtccataaaa aaccattcgg tcttgaggcc
      901 gagcccgcct gagaagccca gccggctgcc gtcgttaagg tcgttctggt aaggcgaagt
      961 aaagtacttc gcgccggccc ggaagctgaa aatattgtac ctccactcgg ttccgatccg
     1021 cacgtgatgt gttaaagcat aactgttgcg gatcgaggag ttttcgccgt taaaactgta
     1081 atcatacgca tcgaaccggg ccccggaata atcagccagt tcgtaatcgg cgctgacaag
     1141 ccccacattg cctatgataa atgccaggtt tccctgcacc cggtaagggg tttgcaggct
     1201 gtattcgtaa taacccgatg gcgaggtgcg gctgtagatt gtttcgccat cgaggtcagg
     1261 tgtgttgaat tccgacgtca tcgtcacctg ccagtaatcg cgcatattgc cgtaccacgt
     1321 tggggtgtga aacgctgcgc cgacgcggag ccagtcgctc gcccggtaga tcgcccccac
     1381 cttcaggttg aacccgctcc cccgggtctc aagctcctca tagcgccgga aattcttcag
     1441 gtcgctgctt tggatgtccg attcggtgta cacagtcgat tcaaaataac ggatggaagg
     1501 aatgcccagg gtgaggccga gataaagcct gttgttgtag ttgctcccga tgctgaaaac
     1561 atattcgttc atcgaacccc tggagttgat ggttttcgtc tgcaacttat ccacccctgt
     1621 cgggattggc gtgacgtacg aggacgagtc gggatcaccc ggcttcaggt cgagcagcca
     1681 gtcccaccag gccaggttga gatcataggc aaaactgcca tcggggtcct gttcgatctc
     1741 ggtgtatttg atcttgtcgg catcctgagc atagacgtcc agcaatgaat tggtgaaatt
     1801 tgtgccggtc atttcatatc ggtagttgaa atcattcagc cggttcaggc cggtagcgaa
     1861 cgtaacatat ttccaacccg ggcgattggg tttcgactgg actttggatg tcagcaccaa
     1921 accgaggttt ccgaggtaga aattggtccg catatccgaa ccggaggtac cgttgaaaga
     1981 agatgccacg cttcccgtgt gcacagccgg cgttatcgaa aactcagagg tcttgaaaag
     2041 cccgagccct gccgggttgg tgccggcggt agtaaaatcg gcccccaggg cgccgaaagc
     2101 gccgctgagg cccatgtagc gggcggtccc gcctatatcg gtccgggaat accggaaggc
     2161 ttcggtggag gtctgagaca gagttgtagt ggcggcggcc aggataagcg ccgaaatcat
     2221 ccataacttt ttcatactgg tgctttttta gaggttatct tctccctgtg cgtgagcttc
     2281 cgctgctgga tccgctgctg gatccgctgc tgctgccgct gctccgggta ctgcttcctc
     2341 cgctgtagct tccggaggag gaacgggtcg aggtccctga attatacgac ggtgaactgt
     2401 tcgatttagc cggcgtggaa tagctcctgg gctgtgtgcc ggaaggcgaa gtgtaggtcg
     2461 accgggaatt tgtccgtgca ggagtggaat agttctgcga cgggttggta ttgaccgggt
     2521 tcaccttgcg ttccggggtt gtggtgtaga ccttgcgggt gctttcaccg tcctgtccac
     2581 cgctgtatgt cctggaatcc ctggtgctcg ggctgctgta ttcctgtttt gaaggagtgg
     2641 tgcgggcagc cggcggggtg tatgtcttgg gcttttcgta tttcacttcg ggctcgacgc
     2701 ggcggctcgg tgaaacagag gtgccttcgg tacgggttgc agtgcctccg gttgaggccg
     2761 ggcgttcgac tgccggtttg ttatagaccg gtttctcggc cggtgtttcg gtgcgggtag
     2821 caggctgtac ctctttacgg gttgcgtttc cgccatcggg attcacttcg ttgcgggtag
     2881 ctgtgccgcc ggtcgaagga gtgacatcgg tgcgcactgc gttgccgccg ccggtagtca
     2941 cttcactgcg tgtggccgta ccggaaggga tggctgaagt attgggatcg cgtttggcgt
     3001 ctccgcctgc cggatcggaa gatttcaggt cgttgccggt gcggtcggag ccacggtcgg
     3061 cgaggccggt gcggttcgac ccgtcggtgg agccggttcg gcttccgcgg tggccataat
     3121 atccgccatc gggagtcggt tcaggataga tcgggtaata tcccccataa taaccatccc
     3181 agtagccatc ccagtagccg ttgttataac cggcccagta agagccgccc cagtagccgg
     3241 gataaccgta tccccatccg taataataag gattatacca tggatcccag tagctgtaat
     3301 agggcctgta gtgataataa ggataaccgt aatatcccca gctcggtccg aagctcaggg
     3361 agaagctggg cccgcagcat ccgtatccgc tgtagatcga acttcccatg tagtaaggat
     3421 cgtagctgta ataataatta tcggtgtaat agttgttgta atagtcaaat ccggggttat
     3481 cgctgtggaa ccgtttgatc cgggaagaat attcgtaatc gtaatattca tccgaactga
     3541 attccccctc ttcatatccg ggagaataat aatccccctc gtatgcggtg gtcgattcta
     3601 cgggttccga ctgaacggta acgtctttgc tgttgctgac cgtattcgac ggcttgtcgg
     3661 atgtagcgta aacttcatcg tacggggccg acgcattgta ggtcgaagaa caggacgcca
     3721 ggaacagggc ggcgagaacg gttaatacgg ctaaattttt catggttgta tcctccagta
     3781 ttttaattgc cttgtggcac ctgtcaaaga tcttatttga ttatttttgt gccacatttc
     3841 acctgaaaaa agcaaatact ataccaaaat aagaaatggc taaagatttt tctacaaggg
     3901 aagataatta cgcgcagtgg tacaacgacc tggtgatcaa agccgacctg gccgaaaact
     3961 ccgctgtaag gggctgtatg atcatcaaac cctatggata cgcgatctgg gagatgatgc
     4021 aatcatcgct cgacaagatg ttcaaggata cggggcacgt gaatgcctat tttccgctgt
     4081 tcatcccgaa atctttcttc agcaaggagg cctcgcatgt agaaggtttt gccaaggagt
     4141 gcgccgtggt gacccattac cggctgaaaa actcgcccga tggcaaaggg gttgtggtgg
     4201 atcccgaggc gaagctggaa gaagagctga tcatccgtcc cacctccgaa acgatcatct
     4261 gggacaccta ccgcaactgg atccagtcgt accgcgacct gcccatcctg gtcaaccagt
     4321 gggcgaacgt ggtcaggtgg gagatgcgca cccggttgtt cctgcgcacc gccgagttcc
     4381 tctggcagga agggcacacc gcgcacgcca ccgaggccga agctgtcgag gaagcgttga
     4441 agatcctgga cgtttacacc cgttttgccg aagagttcct ggccatgccg gtactgcgcg
     4501 gggtgaaatc gccgaatgag cggtttgccg gagccgtaga gacctactgc atcgaaggat
     4561 tgatgcagga cggcaaagcg ttgcaggcgg gaacaagcca tttcctgggg cagaactttg
     4621 cccgggcatt cgacgtgaag tttttaagca gggaaaataa gctcgagcat gtctgggcga
     4681 cctcgtgggg cgtttcaacc cgcctgatcg gagcgttgat catgacccac agcgacgacc
     4741 atgggctggt gcttccgcct aagctggcgc cgcaccaggt ggtgatcgta cctatctata
     4801 agactgccga gcagcttgcc atgatcaccg aaaaggcgaa cagcataaag aaagacctgg
     4861 aatccaaggg gatcagggtg aagtacgacg ggcgcgacac gcaaaaaccg ggctggaagt
     4921 tcaacgaata cgagttcaaa ggcgttccgg tgcgcattgc cattggcccg agagacctgg
     4981 agaacaacac cgttgaggtg gcccggcgcg acacgcttga aaaagagacg ctgcaggccg
     5041 accaccttgg caccaagata gagcacctgc tccagcagat ccagaagaac ctctaccaga
     5101 aagcgctgaa ttacagggag aacaatacgt tcaaggtcga ttcatggaca gaatttaaag
     5161 aaaagatcga agaaggcggg ttcttgctgg ctcactggga cgggaccacg gaaaccgaag
     5221 agaagatcaa ggatgagacc aaagccacga tccgtaccat tccgctcgac gggccgaaag
     5281 agagcggcaa atgtgtttac tccgggaaac catcagctca aagagtggtg tttgcaaggg
     5341 cgtattgagg atgggtcata agctcgctgc gttcgcgtca ttggctcact ttgttcgcgt
     5401 caaaagctcg ctgcgcgagc gtcataaagt agtcattaag tagtcatttg gtagtaataa
     5461 ggttgttatt tggaatctgg aaccttcacc gcaggtgatc cgatattgag gcagattata
     5521 tttcaaaccg cttgcttttc cctccggaat aacatatctt tattccgtta attcatcact
     5581 ctgcataaga atgtatttat ttgtaagaaa atgaaatact tagttttaat tctggcccta
     5641 ataatacctt ccggaatttt agctcaggaa tttgcaccga ccggcgccat ctggcataac
     5701 tcccagtcag actggattaa tccctattta atcacctaca agactatcga atcagtatcg
     5761 gataccataa taaacgggaa atcatgtaag gtcctgattg aagtggaaag atcatattcc
     5821 gatacagcag caacgttcac ccattacatg tattctgaaa acgatagcgt attcttttac
     5881 aaagacaatg cttttc
//
LOCUS       DAOPNH010000220         5856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15739_length_5856_cov_6.566282, whole genome shotgun sequence.
ACCESSION   DAOPNH010000220 DAOPNH010000000
VERSION     DAOPNH010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15739_length_5856_cov_6.566282"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..488)
                     /locus_tag="PKZ74_11040"
     CDS             complement(36..488)
                     /locus_tag="PKZ74_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPM93332.1"
                     /translation="MDLSKILAISGKPGLYKMLSQTKSGFIVESLTDGKRFPVFAHER
                     VSSLEEISVFTTKEEDLQLKDVFRRMYEKTEGKPAPDANSDSKVIRQFFQDTVAEHDP
                     ERVYVSDMKKAIAWFNLLLEKEMMIFEPEGNEPLQEAEREEERTEAQE"
     gene            complement(521..2341)
                     /locus_tag="PKZ74_11045"
     CDS             complement(521..2341)
                     /locus_tag="PKZ74_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM93333.1"
                     /translation="MKKFLRILAYIKPYWIYALLNVVSNMMVIVFSLVTFVMLIPFLN
                     LLFGIDDLVTTRPEIHLNAQSVIDFLNYIISKIILEQGKVDALIFICLFLFGTFFFRN
                     LFRFLAMFFLVKVRVHAVRDIRDEIYHKILILPLSFYNQRKKGDIMSRITNDVQEIDI
                     SIMHYLEIIFRDPVTIIAYFVTLLIMSPQLTVFVMVLLPVAGYVIGTIGRNLRKESKV
                     GQARFSGLLSVVEETIGGLRIIKAFTAIGFSDIRFRELNRNYSRLMVKVFRTRDLSSP
                     MSEFLSSVVIITVLWYGGRLVLSDSGTITAAVFITYILIFSQIIPPVKAFSTGYYNIL
                     KGVASAERIFEILDAEEVIVEKENPVRISKFKEKIEYRKVSFRYQTDDVLNKIDIEIP
                     KGKIIALVGPSGGGKTTFVDLLPRFYDTVEGGIYIDGVNIKDYRIDDLRSLMGMVTQE
                     SILFNDTVYNNIVFGKQGVTRDDVIAAAKVANAHDFIEAMEEGYDTNIGDRGAKLSGG
                     QRQRISIARAILRNPPILILDEATSSLDSESEKLVQDALLRVMKDRTSIVIAHRLSTI
                     KFADRILVLEKGRIVESGNHDELIKTGGLYKRLHDLQTFA"
     gene            complement(2360..3712)
                     /gene="purB"
                     /locus_tag="PKZ74_11050"
     CDS             complement(2360..3712)
                     /gene="purB"
                     /locus_tag="PKZ74_11050"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPM93334.1"
                     /translation="MELTSSNAISPVDGRYRKQTEPLAYYFSEAAYINYRVFVEIEYF
                     IALCNIPLPQLKDFDRNMFEELRAISRNFTFADAEEVKQIEKTTNHDVKAVEYYVKKR
                     FEKLKLKQYKEFVHFGLTSQDINNTAVPYATRLALHDVLLPLVNEVEETLTRKAKEWK
                     EIPMLARTHGQPASPTRLGKEIYVFADRLKNQLSLLKTVPFGAKFGGATGNFNAHKAA
                     YPEIDWAAFGDQFVNKSLGLTRQKVTTQIEHYDNLAALFDNLRRINTILIDLCRDMWT
                     YISMDYFKQKIKADEVGSSAMPHKVNPIDFENAEGNFGMANAIFSHLAEKLPISRLQR
                     DLTDSTVIRNIGVPIGHMMIAIKSLLKGLEKVILNEEKIKADLEDNWPVIAEAIQTIL
                     RRENYPDPYEKLKKLTRTNKKIDKASFHHFIDGLKIPEKLKMELKKITPENYTGIDLI
                     "
     gene            3889..4680
                     /locus_tag="PKZ74_11055"
     CDS             3889..4680
                     /locus_tag="PKZ74_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPM93335.1"
                     /translation="MNIKQHTKDLLYALKKKQISNSSAFLLCYLKHFWNPKNELERIL
                     DNLFKSKPGAFILQVGANDGFVNDPLYKFIKKYKASGILIEPQKKPFSVPKSIYHKDD
                     IEVINCAIDRVNGIRKLYRLSFSDERWASGLSSFNHDQMVKVINSGMIEQKAKKYKIK
                     LPADKNYWISFDEVESLNFETLEKNLHIKAVDLLMIDTEGFDAEIIKMFDFAKFRPLA
                     VVFEKTHLSDEDYLYCKELLKSFTYDLIEENANCIGIYNHKEWIN"
     gene            4668..5762
                     /locus_tag="PKZ74_11060"
     CDS             4668..5762
                     /locus_tag="PKZ74_11060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM93336.1"
                     /translation="MDKLIKILVVSDYNKRVSSRPEAEMFIKLANDPRFRISVMTYPG
                     SYYYGKFRDAGIEVIDFHPDKKLDKKEVEFIRNDIMKKGYDIIHLFNGKAIINGLKAA
                     RGLKVKVVLYRGYTGNIHWYDPAAYLKVLSPRVDKVMCNSQSVKDQIDRQLFFDKTKT
                     VVITKGHDHQWYSDIKPADLAEFGIGQDEFVVTTTADGRKMKGTEYLLRAFELMPPGL
                     NIRLLIMGGGEEFIRRFSGRIKNNAGKVIFTGFRKDNLDIMSSCDVFISPSIKGESFQ
                     KSVAEAMNLGVCPVITDIPGNEGMVIDKKCGLIIPSRDAVAIKDALLDLYSDRARCRE
                     FAKAAKKHISENFSLDKTVDTLAGFYQSLV"
BASE COUNT         1569 a         1304 c         1364 g         1619 t
ORIGIN      
        1 tcctcaactc ttttcatggg gccggtgtgt gaaggtcatt cctgagcttc tgttctttcc
       61 tcttcccttt cagcttcttg caatggttca ttgccctccg gttcgaaaat catcatctct
      121 ttttccagca gcaggttgaa ccaggcaatt gcttttttca tatcggaaac ataaacccgc
      181 tccggatcgt gttcagctac tgtatcctgg aaaaactgcc ggatgacctt gctgtcggaa
      241 tttgcatcgg gggccggttt gccttcggtt ttttcataca tcctgcgaaa aacatccttt
      301 aactgaaggt cttcttcttt cgtggtaaaa acactgatct cttccagtga gctgacccgt
      361 tcgtgggcaa atacaggaaa gcgctttcca tcggtcagtg attcgacgat aaaacctgac
      421 tttgtctgtg ataacatttt gtaaaggcct ggttttcccg agattgccaa aatcttactt
      481 aaatccatat actttgttta aagcttcaaa attacacatt tcaggcgaaa gtctgcaaat
      541 catgaaggcg tttatacaac ccgcccgttt tgatcagctc gtcgtggtta ccgctttcaa
      601 cgatccttcc cttttccagg acgagaatcc ggtcggcaaa ttttatcgtt gataacctgt
      661 gggcgatcac aatggaagtg cggtctttca tcactctcag caaggcatcc tggaccagtt
      721 tctccgattc ggagtcgagt gaagaggtgg cttcgtcgag gatgaggatc gggggattgc
      781 gcaggatggc ccttgcaatg ctgatgcgct gcctctgccc cccggaaagc ttggccccgc
      841 ggtcaccgat gttggtatca taaccttctt ccatcgcttc aataaaatca tgggcattgg
      901 ctactttcgc cgctgcgata acatcatccc ttgtaacgcc ctgttttcca aatacaatgt
      961 tgttgtaaac ggtatcatta aaaaggatag attcctgcgt gaccatcccc atcagcgagc
     1021 gtaaatcatc gatacgatag tctttaatat tcaccccatc aatataaata cccccttcaa
     1081 cggtatcgta aaaccgggga agaagatcga caaaagtggt cttcccgcct cccgaaggcc
     1141 ccaccagggc aatgatcttc ccttttggaa tttcaatatc gattttgtta agcacatcat
     1201 cggtctggta acggaaactg accttgcgat attcgatctt ctctttgaac ttgctgatcc
     1261 tgaccgggtt ctctttttca acaattacct cttccgcatc gagaatttca aaaattcgtt
     1321 cggccgaagc cactcctttc agaatgttgt aataaccggt tgagaaagcc ttaacggggg
     1381 ggattatctg ggaaaagatc aggatatacg tgatgaagac tgccgctgta attgtaccgc
     1441 tgtcgcttaa aacaagcctt ccgccatacc agagaacagt aataatgacg actgaggaaa
     1501 ggaactcact catcggtgag gaaaggtcac gcgtccgaaa aaccttgacc atcagcctgg
     1561 aataatttct attcagttcg cggaacctga tgtctgaaaa tccgatggcc gtaaatgctt
     1621 tgatgatcct caatcccccg atggtctcct cgactaccga aagcaaaccg gagaaccttg
     1681 cctggccaac ctttgattcc ttcctgagat ttcggccgat cgtcccgatc acataaccgg
     1741 cgacaggcag caggaccatc acaaataccg tcagctgagg gctcatgatc agcagggtca
     1801 caaaataggc gatgattgtc accggatcgc ggaaaatgat ctccaggtag tgcatgatgg
     1861 aaatatcgat ctcctggacg tcgttggtga tccgcgacat gatatcgcct ttttttcgct
     1921 gattataaaa cgatagaggc aggataagga ttttatggta aatctcgtca cggatgtcgc
     1981 gcacggcgtg gaccctcact tttaccagga agaacatggc caggaagcgg aaaaggttgc
     2041 ggaagaaaaa ggtgccgaac aagaacaggc agatgaagat cagggcatcc accttgcctt
     2101 gctccagaat gatcttactg atgatataat tcagaaaatc gatgactgac tgggcattca
     2161 ggtgaatttc cggccgtgtt gtcaccaggt catcgattcc gaacaggagg tttaagaagg
     2221 ggatcagcat gacaaaggtc accagcgaaa aaacaatgac catcatattg gaaacgacat
     2281 ttaacaatgc gtatatccag taaggcttga tgtatgctaa gatcctcagg aatttcttca
     2341 ttctgcgggt gttaattcat cagatgaggt caatgcccgt ataattctcc ggggtgattt
     2401 tttttaattc catttttaat ttttccggga ttttcagtcc atcgatgaaa tgatgaaacg
     2461 acgctttgtc aattttctta tttgttcgcg tcagcttttt caacttttca tatggatcgg
     2521 gataattttc cctgcgaaga atggtctgga tggcctcggc gattaccggc cagttatcct
     2581 cgagatcggc tttgatcttt tcttcattca gaataacttt ttccagccct ttcagaaggg
     2641 atttgatggc gatcatcata tggcctatcg ggacgccaat attccggatc accgttgagt
     2701 cggtcaaatc gcgttgcagg cgcgagatcg gcagcttttc ggcaaggtgg ctgaaaatgg
     2761 cattggccat accgaaattt ccttccgcgt tttcaaagtc aatgggattc actttgtgag
     2821 gcatggcaga cgaaccgact tcgtctgctt taattttctg tttgaaataa tccatcgaga
     2881 tatacgtcca catgtcgcgg caaagatcga tcaggatcgt attgatacgt cgcaggttat
     2941 cgaacagcgc agccaggttg tcgtaatgct cgatctgtgt cgttaccttc tgccgggtca
     3001 ggccgagcga cttgttgacg aactggtcgc cgaacgcggc ccagtcgatt tcgggatagg
     3061 ccgccttatg cgcgttgaaa ttgccggttg ccccgccgaa tttggctccg aacgggaccg
     3121 tcttaagcag ggaaagctgg ttcttcagcc ggtcggcaaa aacgtaaatc tctttgccga
     3181 gccgggttgg cgatgccggc tgtccatgcg ttctcgccag catgggaatt tccttccatt
     3241 ccttcgcttt gcgggtcagt gtttcttcca cttcattgac cagtggcagc agtacgtcat
     3301 gcagagccag cctggtggca tacggaacgg ccgtgttgtt gatgtcctgc gaggtcagcc
     3361 cgaaatggac gaactctttg tattgcttta acttcagctt ctcaaagcgt tttttcacat
     3421 agtactccac ggctttcaca tcgtggttgg tcgttttctc aatctgtttg acctcttcgg
     3481 cgtcggcaaa cgtgaagttg cggctgatgg cccgaagttc ctcgaacatg ttcctgtcaa
     3541 agtctttgag ctgcggcaga gggatgttgc acagcgcaat aaagtactcg atctcaacga
     3601 atacgcggta attgatatag gcggcttccg aaaaataata ggccagcggc tcggtttgtt
     3661 tgcggtaccg tccatcgacg ggcgatatgg cattggatga agtcagttcc atgatttcag
     3721 ctttaaagat tcaccgggta aatttatata caaaccattc gaaatgacaa gtaataattg
     3781 ttttttttta ccaaaatact acaattgcgc tctgtagctt gtttttgact ggtttcaaaa
     3841 gcatctcatt gcgtatttta aaatatcttt gttatccgat tggaagaaat gaatataaag
     3901 cagcacacaa aagacctttt atatgcttta aagaaaaagc agatcagcaa ttcatctgcc
     3961 tttcttttat gctatcttaa acatttctgg aatcctaaaa acgaacttga acggatcctg
     4021 gataaccttt tcaaatcaaa accaggtgcg ttcattctcc aggttggcgc caacgacggg
     4081 ttcgtgaatg atccgttata taaattcata aaaaaataca aggcttcagg catccttatc
     4141 gaaccacaga agaaaccctt ttcggtcccg aaatctatct atcataaaga tgacattgaa
     4201 gtgattaact gtgcaattga ccgggtgaac gggatccgga aactgtacag gctgtccttc
     4261 tcggatgagc gatgggccag cggattgagt tcattcaatc acgaccagat ggtaaaggtc
     4321 atcaactccg ggatgattga gcagaaagcg aagaaataca aaatcaagct tccggcggat
     4381 aaaaactact ggatcagttt tgatgaagtg gagtctttaa attttgaaac acttgaaaag
     4441 aacttgcata taaaagctgt tgatctattg atgatagata cggaaggatt tgatgctgag
     4501 attattaaga tgtttgattt tgctaaattc aggcccttgg cggtagtatt tgagaaaacc
     4561 cacctgtcgg atgaggatta tttgtattgt aaagaactgc tgaaatcgtt tacctatgat
     4621 ctgatcgaag agaatgcaaa ttgtatcgga atctataatc ataaagaatg gataaattga
     4681 taaaaattct tgtggtcagc gattataata aaagagtctc gtcaagacct gaggctgaga
     4741 tgtttataaa actggcaaac gatcctcgat ttcgcatctc tgtcatgacc tacccgggat
     4801 catactatta cgggaaattc cgggatgccg gaatcgaagt tatcgacttt catccggata
     4861 aaaaactgga caaaaaggag gttgaattta tccggaatga tatcatgaaa aagggttatg
     4921 atatcattca tttatttaat ggaaaagcca ttatcaatgg cctgaaagcg gccaggggcc
     4981 tgaaggtgaa ggtagttctc tatcgcggct atacaggaaa tatacactgg tacgatccgg
     5041 cagcttacct taaagtactc agcccccggg tcgataaagt tatgtgcaat tcgcagtctg
     5101 tcaaagacca gatcgacagg caactttttt ttgataaaac aaagaccgtt gtcataacga
     5161 aaggccatga ccatcaatgg tactcggata tcaaaccggc cgacctggct gaattcggaa
     5221 tcgggcagga tgaatttgtg gtgactacga ctgccgacgg ccgcaagatg aaaggtacgg
     5281 aatacctgct cagggctttt gagttaatgc ctcccggttt gaatatccgg ttattgataa
     5341 tgggaggggg agaagagttt atccggagat tcagcggaag gataaagaac aacgccggta
     5401 aggtaatttt tacgggtttc cgcaaggata acctggatat tatgtcctcc tgcgatgttt
     5461 ttatctcgcc gtccatcaaa ggcgaatcat tccaaaaatc ggttgcagaa gccatgaacc
     5521 ttggtgtttg cccggtcatt accgacattc ccggcaacga aggtatggtc atcgataaga
     5581 agtgcggact gataatccca tcacgggatg cggttgccat aaaagatgcg ctcctggacc
     5641 tgtattccga ccgggcccgg tgcagagagt ttgccaaagc agcgaaaaag catatttccg
     5701 aaaacttcag cctcgataaa accgttgaca cgctggccgg attctatcaa agcctggtgt
     5761 gatcagggtg ttatgtttag tgaattttcg tggttttgtg ctttggtggc tacaaaagga
     5821 tttttagaaa gggctcaaca attataaaac aaaaag
//
LOCUS       DAOPNH010000221         5849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15775_length_5849_cov_4.838108, whole genome shotgun sequence.
ACCESSION   DAOPNH010000221 DAOPNH010000000
VERSION     DAOPNH010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15775_length_5849_cov_4.838108"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKZ74_11065"
     CDS             <1..349
                     /locus_tag="PKZ74_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93337.1"
                     /translation="TFQCPFMVYVVDGRVHVDWTDFHTYPNPTQQLRAHLTVDGNRHF
                     HARGSGDLVIDLPPGMHTFELELDDYQINSAVFTEVFEVVQQPHNIPLADWAIFLAGL
                     LITVALIYRLYRR"
     gene            complement(363..1103)
                     /locus_tag="PKZ74_11070"
     CDS             complement(363..1103)
                     /locus_tag="PKZ74_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPM93338.1"
                     /translation="MKLLIMKFTLVFTLFTVHCSLFINPAMSQQNAKYVKTEVKKGKF
                     LESTFLLYLPEGYVKKDPKQWPLVLFLHGAGERGDFIELVKKNGPPMMIEEGKSFPFI
                     VVSPQCPEEQRWSVDVLDMLLNHLAEEYNVDEKCIFVTGLSMGGAGTWDLAMAFPKRF
                     AAIAPVCGRADVSQAEKIRDLPVWVFHGAKDDIVPFSEAENIVNALKAMGSPVKFTLY
                     PEANHDSWTETYNNPELWDWMLQQCKQR"
     gene            1268..3295
                     /locus_tag="PKZ74_11075"
     CDS             1268..3295
                     /locus_tag="PKZ74_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93339.1"
                     /translation="MKKNHILALLLVLPFLVSSQTFLTENFDAGAMPPAGWTIDAHST
                     NWTAEGSVNAGGTAPEARFAWEPDFVGQSRIISPAIDLAGQSQVVLMFKHFLDDYDGL
                     GGYSIGAATRSGGGAWNVVWTIDPMANVGPEEKIINISNSDVGSADFQFCIFFDGDSY
                     NLDYWYIDDIMLFVPYEVDGAMAKITTPAYVSGQAQVEGVVRNLGISPITSMVIEWKV
                     SDDMAYETIFTGLDIAFSESYSFVCDDPFYFPIGGYLLDVRITSVNGSPDLNPGNDFL
                     AKAVSVVSHTVYRKPAFEEFTSSTCAPCAAFNEDFNPWTEDHAGDITLIKYQMNWPGA
                     GDPYYTAEGGQRREYYGVSYVPWPQCNGSYVDYNVGAVEAAFQNAILQPGLAKIAATH
                     TLNGTVMTVNANFLPFANFTNFRGHIIVIENTTTENTGNNGETEFHHVMMKMMPGANG
                     TELNMDDRESATITEVFDLAGTNVEEFDDLSVVILFQDYTSKEIYQSAYSIENKAYAT
                     DSRVTGITYDGMPVPGFDPLVYDYEIELPAGTTEVLMVAAVSSDPNATAIVVPTWDLP
                     GTTVVDCFGEDLLARSTYNIHFSVAVGIGETDKENRISLYPNPASNTLYLKGLTGQNI
                     RIYNITGQLVMTVNNFADHSIDISSLGSGSYTLQAVTEDNTVIYGRFAVMR"
     gene            3409..3948
                     /locus_tag="PKZ74_11080"
     CDS             3409..3948
                     /locus_tag="PKZ74_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93340.1"
                     /translation="MKNSFFPKASFVILLIAATVLSSCNKVDELFLSDSEKLMKDLKG
                     TYNLVSVRYEDLASNDGGNTYYNVLDTTFAATGVLDIQTISKAEYTGILTIQFAGFNE
                     THAINGSASGFDDYDNLWIITNGGENIESMILFNPPASYLEGWLDEHDDDHILLRISE
                     TGVDYSAHKNRYFRFETAK"
     gene            complement(4135..>5849)
                     /locus_tag="PKZ74_11085"
     CDS             complement(4135..>5849)
                     /locus_tag="PKZ74_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93341.1"
                     /translation="EAGMNNRGTNVIGIGDYAGYTNRGDNVTALGERAAQDNTANDIA
                     AFGMFAAHLNTGGQVNAIGRYSAYENHGNQINAIGEGAASNNTGHDVNAIGLQAAEAN
                     AANDINAMGFQAAYTNINWGINALGRRAAAGNMMEKVNALGESAAEYNNGPNLNAFGF
                     AAARYNLGWDINAMGERAAFENSHGNVNAFGFEAAGVNRAEDVNALGYQSADSNLGSN
                     VNALGWQAAFANTQWGLNAFGRRAAAENEGWCINALGESAAEFNLSYDVNALGQFAAQ
                     QNSGWAVNAMGSSAARYNSSYIVNAFGPQACELNSGASVNAMGHLAAQDNDGSEVNTF
                     GAMAGQFNTKSSLNALGNSAAKNNTGSSVNALGPSAAENNTGDDVNALGLYAAVGNTN
                     YSVNAFGVYAAGYNSGHNLNAFGSSAAQFNSGIHVNAIGQSAAANNSGWHVIAVGYES
                     AYYNNGNDAIAIGQMALRGSETNGEGDGNIGIGYQAGMNIGAGFNNIAIGYDVPVPDG
                     DLSDQINIGNSIIRDADGVITLNDVIQLPALTSPPSNPQAGMIYFDGAMLYCYDGDFW
                     QALW"
BASE COUNT         1468 a         1510 c         1369 g         1502 t
ORIGIN      
        1 gacctttcag tgccctttca tggtatatgt agtggatggc cgcgtgcatg tcgactggac
       61 cgattttcat acctacccca atccgacgca acagcttcgt gcccatctga cagtcgacgg
      121 caaccggcat ttccatgcac ggggctccgg agacctggtc attgatctgc cgcccggaat
      181 gcacacattt gaactggagc ttgatgatta tcagattaat tctgcagttt ttacagaagt
      241 atttgaggtg gtacagcaac ctcataacat accactggca gactgggcca tattcctggc
      301 cggattgctt ataaccgtgg ctctgatcta tcgcctctac agaagatagt ttccgcggtc
      361 ttctatcgct gtttgcattg ctgcagcatc cagtcccaca gttcgggatt gttgtaggtt
      421 tcggtccagg agtcgtggtt tgcttcggga tacagggtga atttcaccgg gctgcccatc
      481 gctttcaaag cattgacgat attctcggct tccgaaaacg ggacgatatc atcttttgcc
      541 ccgtgaaata cccaaacggg aagatccctg atcttctctg cctggctcac gtcggccctt
      601 ccgcaaaccg gcgcaatggc tgcaaaccgt ttgggaaaag ccatcgcaag gtcccaggtg
      661 ccggcgccgc ccatgcttag tcccgtcaca aaaatgcatt tttcatccac attatattct
      721 tctgccaggt gattcagcag catgtcgagc acatccaccg accaccgctg ctcttcagga
      781 cactggggcg aaacaacgat gaacggaaat gattttcctt cctcgatcat catgggaggg
      841 ccgttctttt ttaccagctc gataaaatcg cccctttcgc ctgcgccatg gagaaaaaga
      901 acaagaggcc actgcttcgg atcctttttc acatatcctt caggcagata aagaagaaaa
      961 gtggattcaa ggaattttcc tttcttaact tcggttttta catatttggc gttttgctgc
     1021 gacatggcgg ggttaatgaa tagtgaacag tgaacggtga acagtgtaaa gaccagggta
     1081 aatttcataa taagaagttt catggctttt ttttcaaaat tcggaataat agccggatca
     1141 attaccgttg aaataaaatc ctgaatcatt ctaaataagg atgctgataa tgttttaatt
     1201 atggaaaaag catatttgtt tcatcaaatc gaatgcatca tcaataacaa ctcaaatcca
     1261 tttaacaatg aaaaaaaatc acattttagc gttgctgctg gttttaccat tcctggtcag
     1321 ctcacagact ttcctgacag aaaacttcga tgccggcgcc atgccaccgg ccggatggac
     1381 gatcgatgcc cattccacga actggacggc ggaaggcagt gtcaatgccg gaggaacagc
     1441 gcccgaggcc cggtttgcct gggaacccga ctttgtcgga caatcccgga ttatttctcc
     1501 cgccatcgac ctggcaggtc aatctcaggt agtgctgatg ttcaaacatt tcctggatga
     1561 ttacgacggc cttggaggct attctatcgg cgcagcaacc cgcagcggcg gcggagcctg
     1621 gaatgttgtc tggacaatag acccgatggc gaacgtcggg cccgaagaga aaatcatcaa
     1681 tatcagcaat tctgacgtgg gcagcgccga tttccagttt tgtattttct tcgacggcga
     1741 ttcttacaac ctcgattact ggtatatcga cgacatcatg cttttcgtcc cctacgaagt
     1801 cgacggcgcc atggctaaaa taaccacacc agcatatgtc agcggccagg cacaggtaga
     1861 aggcgtcgtc aggaatctgg gtatttcccc gatcacctcc atggttatcg aatggaaagt
     1921 atccgacgat atggcatatg agaccatctt caccggattg gatatcgctt tctccgaatc
     1981 atacagcttt gtttgcgacg atcctttcta tttcccgatc ggaggctatc ttctcgatgt
     2041 caggatcaca tccgtgaacg gctcgcctga cttaaacccc ggtaacgact tcctggcaaa
     2101 ggccgtgagt gttgtcagcc acacggttta ccggaaaccg gcttttgaag agttcaccag
     2161 ttctacctgc gcaccctgtg ccgccttcaa cgaggatttc aatccgtgga ctgaggatca
     2221 tgccggtgac atcaccctga tcaaatacca gatgaactgg cccggcgccg gtgatccgta
     2281 ctacacggca gaaggcgggc aaagaagaga gtattacggg gtttcctatg ttccgtggcc
     2341 gcaatgcaac ggttcttatg tcgattacaa tgtcggcgcg gtagaagccg ctttccagaa
     2401 tgccatcctg caacccggtc tggcaaaaat tgccgccacc catacattga acggcacagt
     2461 gatgacggtc aacgccaact tcctgccctt tgccaacttt accaacttca gagggcatat
     2521 tatcgtcatt gagaacacga ccacagagaa tacaggaaat aacggagaga ccgagtttca
     2581 ccatgtgatg atgaaaatga tgcccggggc aaacggaacc gaattaaata tggatgaccg
     2641 cgaatcggct accataaccg aggtattcga cctggccggt acgaatgtcg aggagtttga
     2701 tgacctttcg gtcgtgatcc ttttccagga ctatacttcc aaagaaatat accagtcggc
     2761 ctattctatt gaaaacaagg cttatgctac tgattcacga gtaacgggaa ttacctatga
     2821 tgggatgcct gtcccgggtt ttgatcctct tgtttatgat tatgagatcg aacttccggc
     2881 cggtactacc gaggtgctaa tggttgctgc cgtttcttca gacccgaatg ccacggccat
     2941 tgttgtaccc acgtgggact tgcccgggac gacggttgtt gattgctttg gagaggatct
     3001 gctggcccgc agcacgtata acatccattt ctccgttgcg gtagggatcg gcgaaactga
     3061 caaggaaaac cggatcagcc tgtatccgaa tccggcaagc aacactttat atcttaaagg
     3121 tttaaccggg cagaacatcc ggatctataa cattaccggc cagttagtta tgaccgtgaa
     3181 taatttcgct gatcattcaa tagacatttc atcgcttggt agcggctctt atactttgca
     3241 agcggtaacg gaagacaata ccgtcattta cgggaggttc gcggttatga ggtagcaaaa
     3301 agggtttcca acccgcgggt tggaaaccct ttttttaaca tcccgttaac aaggacgggt
     3361 gaaagcataa tgtattttcg cggccggtat tgaccaaaca aattcattat gaaaaactca
     3421 tttttcccaa aagcatcatt tgtaatctta ttgattgcag caactgttct aagctcctgt
     3481 aacaaagtcg atgagttgtt tctcagcgat agcgagaagc tgatgaaaga cctgaaaggg
     3541 acctataacc ttgtgagtgt aaggtatgag gatttggcca gcaacgacgg aggcaacact
     3601 tattacaacg tgcttgatac cacatttgca gccacgggcg tactcgacat tcaaacgatt
     3661 agtaaagcgg agtacaccgg gatactgacc attcagttcg ccggttttaa cgagacccat
     3721 gcgataaacg gcagcgcctc cggatttgac gattacgaca atctatggat cattaccaat
     3781 gggggagaaa acattgagtc gatgatcctt ttcaatccgc cggcttccta tctcgaaggg
     3841 tggcttgacg agcacgacga cgatcatatc cttctgcgga tctctgagac cggggttgac
     3901 tattcagcgc acaagaacag gtacttcagg tttgagacag caaagtaatc ccaattaaaa
     3961 tggctggcaa aaaagtccta atatttgccc tttttaggta gtccgacgac tgaagccgtc
     4021 ggttcaatca attctggaac caacggcttt agtcgttgga gcacgaacac atgcaaccga
     4081 cggattaacc cgtcggactg caaactcagc agagcccgtc ggagtatggt ttatttacca
     4141 cagcgcctgc cagaaatctc catcgtagca atacagcatg gccccgtcga aatagatcat
     4201 cccggcctgg ggatttgacg gcggcgaagt aagcgccggc aactggatca catcgttcag
     4261 tgtgatgaca ccgtccgcat cccggataat actgttcccg atattgatct ggtctgacag
     4321 gtctccgtca ggaacaggca catcatagcc gatggcaata ttattgaaac cagccccgat
     4381 gttcattccc gcctggtagc caatcccgat attgccatcg ccttccccat tcgtttcgct
     4441 gcctcttaaa gccatctggc caattgcgat ggcatcattt ccattgttat aataagcgga
     4501 ttcataacca acggcaataa catgccagcc tgagttgttc gcagcagcag attgcccgat
     4561 agcattgaca tgaattcctg aattgaattg ggccgcgctg gagccgaagg cgttaaggtt
     4621 atggcctgaa ttgtatccgg cagcataaac cccaaatgca ttgacggaat aatttgtatt
     4681 gcccactgca gcataaagtc cgagtgcgtt gacatcatct cctgtattgt tctccgcagc
     4741 tgaaggtccg agggcattga cactggatcc ggtattattc tttgcagctg aatttcctaa
     4801 tgcgttcagc gaggatttgg tattgaactg acctgccatc gcaccgaagg tgttgacctc
     4861 actgccgtca ttatcctgtg ctgccaggtg ccccatggca ttgaccgaag cgcccgaatt
     4921 taactcgcat gcctgcggcc cgaaagcatt gacaatataa gaagaattgt atcgtgcagc
     4981 agagcttccc atcgcattca ccgcccatcc tgaattttgc tgagccgcaa actgccctaa
     5041 agcattgaca tcatagctca ggttaaactc agctgcgctt tcgccaaggg cgttaatgca
     5101 ccacccttcg ttttctgctg cggctcttcg tccaaaagca ttcagtcccc attgagtatt
     5161 tgcgaaagcc gcctgccacc caagagcatt aacattggaa cctaagtttg aatcggccga
     5221 ctgatagcct aatgcattga catcttccgc acggttaacg ccagcggctt caaatccgaa
     5281 agcattgaca tttccatggg aattttcaaa tgcagcccgt tcacccatgg catttatatc
     5341 ccatcctaaa ttgtaccttg ccgctgcaaa tccaaaggca ttcaggttcg gcccgttatt
     5401 atattctgcg gcagattcgc ccaatgcatt gactttttcc atcatattac cagcagcggc
     5461 ccgtcgcccc agtgcattga ttccccagtt aatgtttgta taagcagcct ggaaacccat
     5521 ggcattgatg tcgttcgctg cattggcctc ggcagcttga aggccgatgg cattcacatc
     5581 atgtcctgtg ttattggatg ccgctccttc gccgatggca ttgatctgat tgccatgatt
     5641 ctcgtaagca gaatacctgc caatggcatt aacctgtccg cctgtattca gatgggcggc
     5701 aaacatcccg aatgccgcaa tatcgtttgc ggtattgtcc tgcgcagctc tttctcccag
     5761 tgcggtaaca ttgtctcccc tgttcgtgta acccgcatag tcaccaatgc cgatcacatt
     5821 ggttccccgg ttgttcatgc cggcttccc
//
LOCUS       DAOPNH010000222         5836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15827_length_5836_cov_5.894309, whole genome shotgun sequence.
ACCESSION   DAOPNH010000222 DAOPNH010000000
VERSION     DAOPNH010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15827_length_5836_cov_5.894309"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..5559
                     /locus_tag="PKZ74_11090"
     CDS             214..5559
                     /locus_tag="PKZ74_11090"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPM93342.1"
                     /translation="MIGPGLSAGLASSRADGFISGYVFNYYGVAISGATIGKENGPFT
                     TSDNNGYYILDRLPEGEHDIGCGKPGYNFSWITVTVISADTTYQNFTLTQPEMVINPL
                     LIEQTLNPGEYITTYINILNTGTGSLHWESVIDYSAQSREINSEWLTMGSYQGDVEPF
                     GGLANVPVYLNATGSVSGQTYSAAIMFSSVPNVGNITVPVTMTVMGNELIPPENLEVS
                     LLDNVDGIVDLSWEWSGDAFQFFLIRRNGQIIATPTQTSFQDILENHGIFCYTVQAVY
                     HEGVTSPAGPACIEWPDPLLQINPVELEGAVWPGYEVDVFTTLSNQGEGTLSFSFPEF
                     AALALAGNSSVRGAGGPDDFGYVWIDNDEPEGPSFKFLDISTTGNPVFGISDDNVVGP
                     FPVGFDFPYYGENKTQFWINSNGAVGFTPNYITLGNTGIPTNSSVYRDFIAWLWDDLV
                     FITGTSQVFYQTVGNKLYIQFKNIQHFNQDDLYINAEIILNKNGKIIVIYEDIDEGVI
                     LNTCTVGLQSHDPEVGLQVAFNQEYLHDDLVILYSVPADFITQVTPASGTIPQGTSTV
                     VTITYDSKDYGPGAYSDDLLLFTNDIENDQMVLENTMYVYLPGIFSGVVTDMDNDEPL
                     NGVSVTAGPFQTTTGENGEYTLFVDEGRYDVVFGKLGYIPVTIYDTATAQGNITPVNT
                     GLWDMNYAPGFVSASVNAGETWCNVAWSLPNGPYEVIMDDGEADDFFVYAQAGSWSAV
                     KFMPTGYPALVLGGKFYVGDGSFPGPFLGTDFGVAVFDDDGVDGKPGTMLDSTGVTVN
                     NSGWVSLDGLNVLITEGSFYLAMYQAGNVPFAAPIGIDSDNPVQYRSYIQFQDNGWML
                     SPLQDFMIRAWISGPENDMKMPVPQLSLKAIPKIPSSWNKYAYTKSGNIPLLVPGYER
                     YEFELTGVNRPAVRDVVSYQVVRFSDFDPDLPPQTGNETILDTLTDLSYPDSAFGSLQ
                     QGWYAYGVRALFTSGLYSDFRISNTVGRMMDCTAGFSISLSTGEEPSGVEISMTGYDF
                     PYATITETTDPDGNAILEPVWKGHYDIRIYKIGYDPYLITDEYIEDDVSFEVVLSEKK
                     YPPTCLQVDSLSLMGNWCEPLFSLLDEDFESAVFPPSGWQNLTSSQAGGWMRGSNGSS
                     PNWTIPAHDSFYAYVNDDAAGSESDGCCDYLVTPALDLRESEGFVLSFDSYYDGFYGQ
                     LASVEYSTDGGGSWETLYNLPPDTSWHSMDVDLSAFSGQNGYSEIWLAFHADDGGEYA
                     SGWAVDNVRVGVPAPAAGYLDFAVFLDGALLGMTSQTSWDFAPLPYGQTYTASVAARY
                     SSGLSEKDYDTFRSVYLFPPKNLTGSAPDDAVILHWDPPGVVVPFNLLGYNIYKNGAL
                     LFYKDHEGGWEPQEYVEPEMQPGIYHYEVTGVYDLASYGFPGETGESMKEGPETVTVD
                     YCTELEFLETWEIESFNETSWNSDGDNWFVNGRAGNPVPCAEFSQVPVVTDYFLALES
                     YPICAVGITEGDIWLTFDIAGYSVLNTGEEQMLVQVWNWESQIWSTVGEFSNIDGSFD
                     WTPGRINIRPYVMNSIFKIRFVARGINSANIRGWFLDNIHVFRSCTAPSELQTDPYSN
                     DGIHLSWLLGDYNGTRELNAVNIYRSANGGAFQALATLTGGHEYTDPDSNLSFGSFYC
                     YKVNAIWQSETDYCESTYSNEACIVWTGMEPPQNEVSGDISIYPNPAGDYFVVSSAMP
                     VRKVEVFSPEGKKLIEKQDVISGTRFSTTHLGSGIYIVHVSGDQWQAGRRLVIIR"
     gene            5597..>5836
                     /locus_tag="PKZ74_11095"
     CDS             5597..>5836
                     /locus_tag="PKZ74_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM93343.1"
                     /translation="MFSVQNISIHFGGTPLFRNVSFIINQKDRIGLAGKNGSGKTTLL
                     RIITGIQVPDEGEVVIPNEKTLGYLPQELHLQNTRP"
BASE COUNT         1440 a         1383 c         1442 g         1571 t
ORIGIN      
        1 ataatatcac aggataactg ataaatgaaa ggccggcctc gccggccttt tttttcgtca
       61 ttatccgaaa acatgcaacc aatcttaatt atttaatgtc ttatcattgt aacattaacc
      121 tgaaaaataa gtatatcagt aagtcatgcc gcattattaa tccggcttct tgaaatgagg
      181 ataaccatta aaatatccct tttatttttg attatgatcg gacctggcct gtctgcaggt
      241 ctggcctcat cgcgtgctga tggctttatt tcaggttatg tatttaatta ttatggtgtt
      301 gcaatatcag gcgctaccat aggcaaagag aacgggccgt ttacaacctc cgacaacaac
      361 gggtactata tattggaccg tttacccgaa ggggagcatg atatcggctg cggcaaaccg
      421 gggtacaact tctcatggat taccgtcacc gtaatttctg ctgatactac ctaccagaat
      481 ttcaccctca cacaaccgga aatggtgatc aatcctttac tgattgagca aacccttaat
      541 ccgggtgaat atatcacaac atatatcaat attcttaata ccggaacggg gtcattacac
      601 tgggaatccg tcattgatta ctctgcacag agcagggaga tcaattcgga atggttgacc
      661 atgggaagtt accagggtga tgttgagccg tttggcggcc tggccaacgt tccggtctat
      721 ttaaatgcaa cgggttctgt ttcgggacag acctattcgg ccgctatcat gttttcttcg
      781 gtcccgaatg tgggtaatat tacagttccg gttactatga cggtaatggg gaatgagctg
      841 atccctcctg aaaaccttga agtgagccta ttagataatg tcgatggaat tgtcgattta
      901 tcgtgggaat ggagcgggga tgcctttcaa tttttcctga tccggcgaaa cgggcaaatt
      961 atcgctaccc caacacagac ctcctttcag gatattcttg agaaccatgg aatcttctgc
     1021 tatacggttc aggctgttta ccatgaaggc gttacatcac ctgccggacc tgcatgcatc
     1081 gaatggcccg atccattgct gcaaataaat cctgtcgaac tggaaggtgc agtctggcct
     1141 ggttatgagg tggatgtttt taccactttg tccaaccagg gagaaggtac attgtcgttt
     1201 tcctttcctg aatttgctgc cctggccctg gccggcaact cttcggtccg tggcgcaggt
     1261 ggcccggatg actttggata tgtctggatc gataacgatg agccggaagg cccctctttc
     1321 aaattcctgg atatctccac caccggaaat cctgttttcg gtatctccga cgataacgtc
     1381 gtagggcctt ttccggtcgg ttttgatttt ccgtattatg gagaaaataa aacccagttt
     1441 tggataaatt caaatggtgc tgtcggtttt acccccaatt atattaccct gggaaatacc
     1501 ggtattccaa caaacagctc ggtttacagg gattttatcg catggttgtg ggatgacctt
     1561 gtttttatta ccggtaccag ccaggtgttc tatcaaacgg tgggtaacaa gctctacatc
     1621 cagtttaaga atatccagca ttttaaccag gatgatctct atatcaatgc cgaaatcatc
     1681 ctcaataaaa atgggaagat cattgttata tatgaagata ttgatgaagg tgtgatcctg
     1741 aacacctgca ccgtcgggct tcaatcccat gacccggagg taggattgca ggtggcattt
     1801 aaccaggaat atcttcatga tgacctggtt atcctttaca gtgttccggc cgattttatc
     1861 acccaggtga ctccggcctc cggtaccatc ccgcaaggta cttcaaccgt cgtaacaatt
     1921 acctatgatt caaaggatta cgggccgggt gcttattctg acgatttgtt gctgtttacc
     1981 aatgatatcg aaaatgatca gatggtattg gagaatacca tgtatgttta tctgcccggt
     2041 attttcagcg gtgtcgtcac ggatatggat aacgatgaac ccctcaatgg cgtttcggta
     2101 actgccggac cctttcagac aacgaccggc gaaaatggtg aatacactct ttttgtagat
     2161 gaaggaaggt atgatgtggt gttcggtaaa ctgggctata ttccggtcac gatatatgat
     2221 accgctaccg ctcagggaaa tattaccccg gttaatacag gcctctggga catgaattat
     2281 gcccccggat ttgtcagcgc ctcggtcaat gccggtgaaa cctggtgcaa tgtcgcctgg
     2341 agcctgccaa acggtcctta tgaagtgatc atggatgatg gagaagccga tgattttttc
     2401 gtttatgcac aggccgggag ctggtcggcc gttaaattta tgccaacagg gtatccggcc
     2461 ctggtcctgg gaggaaagtt ttatgttggt gatggcagtt ttccggggcc tttcctgggg
     2521 accgattttg gagtagccgt ttttgatgat gatggcgttg acggaaaacc cggcaccatg
     2581 ctcgattcta ccggcgttac cgtcaataat tcgggatggg tcagcctcga cggattgaat
     2641 gttctgatta ccgaaggaag tttttacctc gccatgtacc aggcaggaaa tgtccctttt
     2701 gccgcaccca tcggcatcga ttccgacaat cccgtccaat atcgcagtta tatccaattt
     2761 caggataatg gatggatgct gtcgccactc caggatttta tgatcagggc ctggatctcg
     2821 ggaccggaga atgatatgaa aatgcccgtt ccgcagttat cactgaaagc gatccccaaa
     2881 atcccatctt cctggaataa atatgcttat accaaaagcg ggaatatccc tttactcgtt
     2941 cccggctatg agcgatatga attcgagctg accggtgtga acaggcctgc agtcagagat
     3001 gtggtctcat accaggtagt ccggttttcg gattttgatc ccgatctgcc tccccagacc
     3061 ggtaatgaaa ccattctgga tacgctgacc gacctgtcgt accccgacag cgcatttggg
     3121 tcattgcagc aaggctggta tgcttatggg gtcagggcat tgtttaccag tggattgtac
     3181 tccgatttca ggatttccaa tacggttgga agaatgatgg attgcaccgc cggctttagt
     3241 atttcgctca gtacggggga agaaccttcc ggagtggaaa tttcgatgac cgggtatgat
     3301 tttccttatg caacgattac ggaaacaacc gatccggacg ggaatgcaat actggagccg
     3361 gtttggaaag ggcattatga cattcggata tacaagatcg gatatgatcc atatcttatt
     3421 acagatgaat atattgaaga tgatgtttct ttcgaagttg tcttaagtga gaagaaatac
     3481 ccgcccacct gccttcaggt tgattccctc tcgctgatgg gtaattggtg cgagccactg
     3541 ttctcattgc tggatgagga ttttgaatct gctgtttttc caccctccgg atggcaaaac
     3601 ctgacctcaa gccaggctgg aggctggatg aggggctcca atggcagcag tccgaactgg
     3661 acaattccgg cgcatgacag cttttatgct tatgtcaatg atgatgccgc cgggtcggag
     3721 agcgatggat gctgcgacta cctggtcaca ccggctcttg acctgcgaga aagtgaaggc
     3781 tttgtcctca gtttcgatag ttattatgat ggcttttacg gacaattggc atcggttgaa
     3841 tattccaccg acggaggcgg ttcttgggaa actctttaca acctgccgcc cgatacctcc
     3901 tggcattcga tggacgtcga cctttcggca ttcagcggac aaaacggcta ttcagagatt
     3961 tggctggctt tccatgccga cgacggcggc gagtacgctt caggctgggc agtagataat
     4021 gtcagggtcg gtgtgcctgc tccggctgca gggtacctcg attttgcggt gttcctggat
     4081 ggggccctgc tgggcatgac ttcacagact tcctgggatt ttgctcccct gccttacggg
     4141 caaacataca cggccagcgt cgcagcaaga tattcaagcg gactttcaga aaaagattac
     4201 gacactttcc gctcggtcta tctttttcca ccgaaaaacc tgacgggctc tgctccggat
     4261 gatgccgtta tcctccattg ggacccgccg ggagttgtcg ttcccttcaa tctgctggga
     4321 tacaacattt acaagaatgg cgccttacta ttttataaag atcatgaagg aggctgggag
     4381 cctcaggaat atgtggaacc ggaaatgcaa ccgggaatct atcactatga ggtaaccggt
     4441 gtctacgacc tggcttccta tggatttccg ggcgagacgg gcgaatcgat gaaagaaggc
     4501 cctgaaaccg taacagttga ttattgtaca gaacttgaat tcctggaaac ctgggagatt
     4561 gaaagtttca atgaaaccag ctggaattct gacggtgata actggtttgt caatggccgc
     4621 gccggtaacc cggtaccctg cgctgaattc tcccaggtgc ctgttgtaac ggattatttc
     4681 ctggctctgg aatcgtatcc catatgtgct gtaggcatta ccgaaggcga catctggctg
     4741 acttttgata tcgccggtta ttcggtcctg aatacggggg aggaacaaat gctcgttcag
     4801 gtctggaact gggaatcaca gatatggtca accgtcgggg aattcagcaa tattgacgga
     4861 tcctttgact ggactcccgg ccggatcaat atcaggccgt acgttatgaa ctcgatcttt
     4921 aaaatacgat ttgtagccag agggatcaat tccgctaaca tccgcggctg gttcctcgat
     4981 aatatccatg tgttccgctc ctgtaccgca ccatctgaac ttcaaaccga tccatactcc
     5041 aacgacggca ttcacctctc ctggctcctg ggcgattaca acggcacccg tgaacttaat
     5101 gccgtaaata tctaccgaag cgcaaacgga ggcgcattcc aggccctggc aactttaaca
     5161 ggcgggcacg agtacaccga tcccgactca aacctgtcgt tcggctcatt ctattgttat
     5221 aaggtaaatg ccatctggca aagcgaaacg gattactgcg aaagtactta ctccaacgaa
     5281 gcctgtattg tatggacagg tatggaaccg ccccaaaatg aggtttccgg tgatatttct
     5341 atctatccga atcctgcagg agattatttt gtggtctcat cggctatgcc cgtgcgcaag
     5401 gttgaggttt tcagcccgga agggaaaaaa ctgatcgaaa aacaggatgt tatcagcggg
     5461 acccgattca gtaccaccca tctcggttcc ggaatataca tcgtgcatgt ttcgggcgac
     5521 caatggcagg ccggccggag actcgtcatc atccgatagc gaaaattccc tatttttgcc
     5581 gccataccgg ttaaacatgt tttctgtcca gaatatatcc atccatttcg ggggtacacc
     5641 cctgttccgg aatgtcagct ttatcatcaa ccagaaagac aggatcggtc tggcaggcaa
     5701 gaatggcagc gggaaaacca ctttgctcag gatcatcacc ggtatacagg ttcccgatga
     5761 gggtgaagtc gtcatcccaa atgaaaaaac gctgggttat ctgccgcagg aactgcacct
     5821 gcaaaatacc cgcccg
//
LOCUS       DAOPNH010000223         5809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_15921_length_5809_cov_7.573688, whole genome shotgun sequence.
ACCESSION   DAOPNH010000223 DAOPNH010000000
VERSION     DAOPNH010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15921_length_5809_cov_7.573688"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..438)
                     /locus_tag="PKZ74_11100"
     CDS             complement(25..438)
                     /locus_tag="PKZ74_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPM93344.1"
                     /translation="MKNFLFNSGNHKAMASFALLVLRASFGLMMIVHGWDKLAHYNSY
                     ASGFPDPLHIGNEFSFWLVVFAEFFCAILIVLGLMTRFAAIPLIIAMAVVAFVINIKE
                     PFADRELSMLYLSAYLAILIFGAGSYSLDKAISGK"
     gene            complement(563..635)
                     /locus_tag="PKZ74_11105"
     tRNA            complement(563..635)
                     /locus_tag="PKZ74_11105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Thr,seq:cgt)
     gene            818..1945
                     /locus_tag="PKZ74_11110"
     CDS             818..1945
                     /locus_tag="PKZ74_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93345.1"
                     /translation="MKIVRKFQDLGSIIQPNKVLLILGPRQVGKTTLMKDYLKKSPLR
                     YKFITGDDLLIQKPFVTQSLSEIKSFCEGYDLLAIDEAQKIPNIGAAMKLMVDNIPGL
                     HVIATGSSSFELAGQTGEPLTGRKTTLLLYPVAQMELLNHFNKFELGQKINDFLVYGG
                     YPEVITRDSLEEKRKVLNEITGSYLFKDILAFERIRNPQFLIDLLRMLAFQIGSEVSV
                     NELSVKLGIDNKTVKKYIELLEKSFILHTVRGYSGNLRKEISKKPKFYFYDTGIRNAI
                     IANFNPPETRNDLGQLWENFIVMERIKKQTYLPLYANNYFWRSWTGKEVDFIEEREGK
                     LFGFEIKWSNARTKVPSLWLESYPEATFEIINKDNYLEFIT"
     gene            2018..3622
                     /locus_tag="PKZ74_11115"
     CDS             2018..3622
                     /locus_tag="PKZ74_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPM93346.1"
                     /translation="MILKDIKILILLLILFVPGMMRADHITVQGEVSGLWNADTVMVM
                     GDISVPDGEALMIAPGTVIKFQGDFHFAVNGAVEAIGEPGSMIVFTPNDTTGFSNDTI
                     PGGGWGGIRFDHNRLSNGVSEFYHCRFSYAKKVSADPATGHGGAFYIKAWDKVNFHGC
                     VFENNFSTYNGGAVYLDSSDVSIQHCTFTGNRCGPASAPWGYGGAVCSDNSVPDIRWN
                     IFNGNSSTGVGGALAVRFTDCNVYNNIFEGNVSALGGAFGFLHIPQCDNRISNNLIVN
                     NQSAFFGGGVANLNASPYYINNTIIYNSSTYGGGFYCKDSVSPDFYNTVIRGNTAAVG
                     SQGYLFEVYSQADFFYCNVEDGPGMFGGSGGGEAFFGAFESCLDTLPGFAGEGDYPYS
                     LNPSSPMIDAGAADTAGFLLPETDLAGVTRVYNNRIDIGAYEYLPVSVPAPDIDIVTG
                     IATVFPNPFRDDVNVKIDVIEGKKVFIEVFDMAGRRLFHSEEYCRKGNNQFVLHLPDT
                     PDGLLTLRLISGNGLYFFKLYRISHR"
     gene            complement(3687..5111)
                     /locus_tag="PKZ74_11120"
     CDS             complement(3687..5111)
                     /locus_tag="PKZ74_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPM93347.1"
                     /translation="MKRFLLLLFTVHCSLFTLHAQYTPDPDPQAMAKLQAWQDLKFGL
                     LMHWGPYSQWGVVESWSICAEDEGWCRRKSDNYTEYKKDYERLKNTFNPVHFDPAAWA
                     KAARHAGMRYVVFTTKHHDGFCMFDTKQTDYKVTDPGCPFSAHPKANIAAEIFNAFRN
                     EGLWTGAYFSKPDWHCPDYWWPNFATPDRNVNYDIKTYPDRWENYVQFTHNQIMELMT
                     DYGPIDILWLDGGWVRTLTDAEISKYINAPDYKFMHIQSQDIRMDELVQKARARQPGL
                     IVVDRAVPGKNQNYLTPENEVPEKTLPYPWESCIISGGSWSWVPDAKYKTPAQVIHLL
                     ADIVAKGGNLLLNIAPGPDGRWDAGAFEMLQGVGEWMEVNSVAIYNTQPLAPYKEGKV
                     CLTRGKDGTVNLIYLAGEGETGPPAKIWFSTLDMPAGATARMLGSRESLSWEKVANGV
                     IIHIPESLQKKPPCSSAWVVRFSN"
     gene            5475..>5809
                     /locus_tag="PKZ74_11125"
     CDS             5475..>5809
                     /locus_tag="PKZ74_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93348.1"
                     /translation="MKNFIAHSKNGSGKEHILTDHLEGVAAQLENFLKYLPYKKIFKV
                     TGLFHDFGVSQIPSHIAQKSWMDQSENEKLIYRTHVAKSVELARRFDPTLTERSLMMI
                     GQTHEKFNGT"
BASE COUNT         1562 a         1330 c         1392 g         1525 t
ORIGIN      
        1 aatgaccatc catgaccatc aaccctactt cccgctgatg gctttgtcaa gcgaatagct
       61 tccggccccg aagatcagga tggcaaggta ggccgaaagg tataacatgg ataactctct
      121 atctgcgaaa ggctctttta tattgatgac gaacgcaacg acggccatgg cgatgatcag
      181 cggaatggcg gcaaagcgtg tcattaatcc cagtacgatc aggatggcgc agaaaaactc
      241 ggcgaagacg accagccaga aagagaattc attgccgata tgcaaaggat cgggaaaacc
      301 ggatgcgtag ctgttgtagt gggccagttt gtcccatccg tgcacgatca tcatcaggcc
      361 gaacgaggcc ctcagcacca gcagcgcaaa gctggccatc gctttatggt tgccggaatt
      421 gaaaaggaaa tttttcatat attaattaac tatttacact atgaaatgca tcaattataa
      481 atgataaatg caatataaaa cgacaaatgt aaaatgaaat gaaaaatgca caatgcaaaa
      541 tgcaaaatga aaaatggcag tgagcctctc aggggactcg aacccccgac ctgctcatta
      601 cgagtgagct gctctaccaa ctgagctaaa gaggcgaaaa aatcgccggc aaaaatactt
      661 aaaatagttc aaagttcaaa gtgccaggcg aaattgcttt actccgctaa gtaaataaga
      721 atgctataag tttatattat ttggactaat aatccaaata atatcagtaa tattgggaat
      781 atgtcgaaaa taattctata tttgaagaaa attcaagatg aagatagttc gaaaatttca
      841 ggatctcgga agcatcatcc agcctaacaa agtattgctt atacttgggc ccagacaggt
      901 cgggaaaaca acgctgatga aggattatct aaaaaaaagt cctttgaggt acaaattcat
      961 aactggcgat gacctgttga ttcagaaacc atttgtcacc caatcgctca gcgaaataaa
     1021 atcattttgt gaaggttatg accttctggc aatagatgaa gcgcagaaga ttcctaatat
     1081 aggcgctgca atgaaattaa tggtagataa tattccgggg cttcatgtta ttgccaccgg
     1141 atcgtcatcc tttgaactgg ccgggcaaac cggagaaccg cttaccggga gaaaaacaac
     1201 attattgctt tacccggttg cccagatgga actattgaat catttcaata aatttgaact
     1261 cggtcagaaa ataaatgatt ttcttgttta cggaggatat cctgaagtta ttacccggga
     1321 ttctctggag gaaaaaagaa aagtcctcaa tgaaatcaca gggtcctatc tttttaagga
     1381 tatcctggct tttgagagaa tcaggaatcc gcagtttctg atcgatttac tgcgtatgct
     1441 ggcctttcag atcggcagtg aagtttcagt gaatgagcta tctgttaagt tgggaataga
     1501 taataaaacc gttaaaaagt atatagaatt attggagaag tcatttattc tccacaccgt
     1561 caggggctat agcggaaacc tgagaaaaga aatttcgaaa aagccaaaat tctatttcta
     1621 tgatacgggc atacgcaatg ctattatagc caatttcaac ccgcctgaaa ccaggaatga
     1681 ccttgggcag ttatgggaaa atttcatcgt tatggaacgg attaagaaac agacctattt
     1741 gccgttatat gctaataatt atttctggag atcctggacc gggaaggagg ttgattttat
     1801 tgaagaaaga gaaggaaagc tttttggttt tgagataaaa tggtcgaatg ccagaaccaa
     1861 agttccttct ttgtggcttg aatcctatcc tgaagcgact tttgagatta taaacaagga
     1921 taactatctg gagttcatta cataatatca aattgagcag caagccatta tttctaactt
     1981 tgccgttcgg cttcattatt gtcaaaacca tataaatatg attttaaaag atattaagat
     2041 cctgatcctt ttattgatcc tgtttgtccc cgggatgatg cgggccgacc atatcaccgt
     2101 acagggagag gtttccggcc tgtggaatgc cgatacggta atggttatgg gcgatatcag
     2161 cgttcccgat ggagaggcgc tgatgattgc gccgggcact gtgatcaaat tccagggcga
     2221 tttccacttt gccgtcaacg gggcggtcga ggccatcggt gaacccggat ccatgatcgt
     2281 tttcaccccc aacgacacga caggattttc gaacgacaca atcccgggag gaggatgggg
     2341 cggcattcgc ttcgatcata accgcctgag caatggcgta tccgaatttt accattgtcg
     2401 tttcagttat gccaagaagg tcagcgctga tccggccaca ggccatggag gggcttttta
     2461 tatcaaagcc tgggacaaag tgaattttca cgggtgcgtt tttgaaaaca atttttcaac
     2521 ctacaacggc ggggcggttt acctggattc gtccgatgtt tcaattcagc actgcacctt
     2581 taccggcaac cgctgcgggc cggcttcggc gccctggggc tatggcggag cggtctgttc
     2641 cgacaactcc gtgcccgata tccgctggaa tatcttcaac ggcaactcct cgacgggagt
     2701 cggaggggcg ctggcggtga gattcaccga ctgcaacgtc tacaacaata tctttgaagg
     2761 aaatgtcagc gccctgggcg gagcgttcgg tttcctgcat atcccacagt gcgacaaccg
     2821 gatcagcaac aacctgatcg tgaacaacca gtcggctttt ttcggtggcg gcgtcgccaa
     2881 cctgaatgcc agtccctatt atatcaacaa cacaattatt tataattcat cgacatacgg
     2941 gggaggattt tactgcaaag actccgtctc tcccgatttc tacaacacgg tcatccgggg
     3001 aaacaccgcc gctgtcggat cccaggggta ccttttcgag gtctattccc aggccgattt
     3061 tttctattgc aatgtggagg acggccccgg catgtttggc ggcagcgggg gaggagaggc
     3121 gtttttcgga gcctttgaat cgtgcctcga caccctcccg ggttttgccg gcgagggcga
     3181 ttatccttac agcctcaatc cttcgtcacc aatgatcgac gccggtgcgg ccgacaccgc
     3241 cggattcctg ttaccggaaa ccgacctggc cggagtaacc agggtttata acaaccggat
     3301 agatatcggc gcctatgagt acctccccgt ctccgtcccc gcccctgaca ttgatattgt
     3361 tactggtatt gccactgttt tccccaatcc gttccgggat gatgtgaatg tgaaaataga
     3421 cgttattgaa ggaaaaaagg tatttatcga agtttttgat atggcgggca ggcgattatt
     3481 tcactctgaa gaatattgca ggaaaggaaa taatcagttc gttctccatt tgccagacac
     3541 acctgatggc ttgctgacct tgcgattgat ttccgggaac gggttatact tttttaagct
     3601 ttatagaatt tctcacagat aacacagatt agtcacagat ctgtgtcaat ctgtgctgtt
     3661 catccgtgaa atctgtgaga aaacaatcaa ttcgaaaatc tcaccaccca ggccgaagag
     3721 cacggcggtt tcttttggag gctttccggg atatggatga tcacaccgtt cgcaactttt
     3781 tcccaggata aactttcacg gcttcccagc atccgagccg tggcgccggc aggcatatcc
     3841 agcgtggaga accaaatctt tgcgggcgga ccggtttctc cttcgcctgc caggtagatc
     3901 aggttgacag tgccatcctt tccccgggtc aggcacacct tgccctcctt gtaaggcgcc
     3961 agcggttggg tattgtaaat tgcaacactg ttgacctcca tccactcgcc cacaccctga
     4021 agcatttcaa acgcgcccgc atcccagcgg ccatccggtc ccggcgcgat attcagcagc
     4081 aggttgccgc ccttggccac gatatcggcc agcaggtgga tcacctgtgc cggtgttttg
     4141 tattttgcgt cgggcaccca cgaccagctt ccgccggaga tgatgcacga ttcccacggg
     4201 tatgggagcg ttttctcggg cacctcgttc tccggggtca ggtagttctg gttcttcccc
     4261 ggcaccgccc ggtcaacgac gatcaatccc ggctgccttg ccctggcttt ttgcaccagc
     4321 tcatccatcc ggatatcctg gctctggatg tgcatgaact tatagtcagg cgcgttgata
     4381 tactttgaga tttcggcatc ggtgagcgtc ctcacccatc cgccgtcgag ccagagaata
     4441 tctatcggcc cgtagtccgt cattagttcc atgatctggt tgtgggtgaa ctgcacataa
     4501 ttttcccatc tgtcggggta cgttttgatg tcgtagttca cattccggtc gggtgtggcg
     4561 aagttgggcc accagtaatc ggggcaatgc cagtcgggtt tcgagaagta ggcgccggtc
     4621 cacagccctt cattccggaa agcgttgaag atctcagcag caatattggc cttgggatgg
     4681 gcgctgaacg ggcatcccgg gtcggtcact ttgtaatcgg tctgtttggt gtcgaacatg
     4741 cagaagccgt cgtggtgctt cgtcgtaaag accacgtagc gcattcccgc gtgcctggcg
     4801 gctttggccc acgccgccgg gtcgaaatga accgggttga acgtattttt cagcctttcg
     4861 taatcttttt tatactccgt ataattatcc gattttcgcc ggcaccatcc ttcatcttcg
     4921 gcgcagatcg accacgattc caccacgccc cactggctat acggtcccca gtgcatcagc
     4981 aggccgaact tgaggtcctg ccaggcctgg agctttgcca tcgcctgcgg gtcgggatcg
     5041 ggagtgtact gggcgtgcag ggtgaacagt gagcagtgaa cggtgaagag aagaaggagg
     5101 aatcgtttca tggcttggga atttggaaat gagacaattt gggaatttgg aaatgagaca
     5161 atttgggaat ttggaaatga gacaatttgg aaattcatta agaattcaaa attaagcatt
     5221 cgaaatcaaa cattcataga aaaaagaaac tcccaccgaa gcgggagtat aggatttaca
     5281 tccttcggca taaagcctta ttgtggtaag atgtattata gcgaatataa tgatcagcta
     5341 tttttgttga taatttttat taataatcaa taatcttttc agaattaata ttactatttt
     5401 taaaatctga tttctgacct ttaatccttt agtcactttg tatctttata aagtaaagca
     5461 ttgctaatca aagaatgaag aattttattg cacattctaa gaatggatcc ggtaaagagc
     5521 atattcttac tgatcatctt gaaggagtag ctgctcaatt ggagaatttt ttgaaatatt
     5581 taccgtataa aaagatattt aaggttacag gattatttca tgattttggt gtttcacaga
     5641 ttccgtcaca catcgcacaa aaatcttgga tggaccaatc cgagaatgaa aaactcatct
     5701 atcgcactca cgtcgcaaaa tccgtggaac ttgctcggag gtttgaccct acgctcacgg
     5761 agcgcagctt gatgatgatt ggacagaccc atgagaaatt caacggaac
//
LOCUS       DAOPNH010000224         5784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16021_length_5784_cov_4.166870, whole genome shotgun sequence.
ACCESSION   DAOPNH010000224 DAOPNH010000000
VERSION     DAOPNH010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16021_length_5784_cov_4.166870"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKZ74_11130"
     CDS             <1..780
                     /locus_tag="PKZ74_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93349.1"
                     /translation="AAGLYPEWLAWPLKWCLDLMLGSVEWIQSWPGAVLRPFLPDAAT
                     TIFSYAAILFLFIMISTRKKKWLLPLMSAIAAVSVLFAVNQYNRIERCALYVYQHKGS
                     LAIDMMKNRRNLFVTDGRMQKDSTRLQMLVEANRIKNRISLTDTLQISEKGQIGRQEY
                     WYSRPFLNYMGKKIVFIHDEWLQGNAGQRVQCDLAILSGQSGFNDPAVLNCVKFERAI
                     LAPDLSYSREERIVKWLNDAGIPFHSVRKDGAFVMEWRSSE"
     gene            complement(805..3090)
                     /locus_tag="PKZ74_11135"
     CDS             complement(805..3090)
                     /locus_tag="PKZ74_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPM93350.1"
                     /translation="MKNLLISVILLAMSVTAFSSELFILNTGSVERTKAAFNQNTFVI
                     HFYNDDFIIGSSDDGSPAGSLIIDNNAWMTDDVHYFILYYPAENRDQYTALVAAYAKI
                     LYDSGNFLAVSTSGENTKQLIPAIHGGIVRIENKAARFPDQSFSYHPGSLQMRDYIAD
                     LIDLVDMDTLEAFVQRLQDFGTRNAYKAGGIQAQNWILAKLQSYGLETELHDFWMPGG
                     NASDNVIATLPGNVYPDEYVIIGAHYDSYAGGSNEPGADDNATGTAGVLEAARILSQY
                     QFDRTIIFAAWSGEEYGLYGSDAWATEAANEEMNILGYFNIDMAGYLQEGDAIHTDII
                     APASANELRQFYKDICAIYLPDFQTSNGMLTGGDSDHTSFNNNGYPGLFPFEDSQNYS
                     PYIHTSNDLIGPSVNNFEQHMTFVKAAIANVVSLSDLLPRPEDLTALSGDQQVELAWD
                     PVAEAASYNIYRDGGEEPYATTEETSFTDTGVENMVSYTYYVTAVFEGSGEESGPSNS
                     VTVMPMPPIILPFFDDFETGGLFWSREGTWGLQQGIYYSSSNSLTESPDEKYEANLEI
                     SSTLRPIDLTGAFSAELSFWTKYEIESGWDFMNLEISVAGLDWEPLANYSGLMEDWTQ
                     LTYSLDDYIGQSDVIVRFRFNSDGFVQEQGMYIDDLEISINGIGIHEKTSDQPIDFRI
                     NPNPATDLLRIDYTLESAGSVEILIADLKGTTVSVLDNSYREKGDHFIHTSLEPLPAG
                     SYMVILRHSGKQSSRKLIITE"
     gene            3201..4025
                     /locus_tag="PKZ74_11140"
     CDS             3201..4025
                     /locus_tag="PKZ74_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HPM93351.1"
                     /translation="MGQTEKPWPKWRNDLHRIIFGTDTFEGRLFDIILIFFISASVLV
                     VFLDSIGYIHKQYGRWLYGLEWFFTIVFTIEYIFRILSLRKPWQYVFSFYGLIDFLAV
                     MPTYISLIFTGGQYLIAIRALRLLRIFRVFKLGRFLWASRYLMVAIRNSRHKIVVFLW
                     FVIIIVVVMGSVMYLVEGPENGFTNIPDSIYWAIVTLTTVGYGDISPQTPLGKAIASV
                     IMIIGYGVIAVPTGIFTAELARMHSSSDKTCNHCQNAQNDPDAKYCKKCGQPLQDP"
     gene            4135..>5784
                     /locus_tag="PKZ74_11145"
     CDS             4135..>5784
                     /locus_tag="PKZ74_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93352.1"
                     /translation="MKKFYSLIIAISLAFPVFSQTYLSEDFSSGTMPPPGWTIFAFQQ
                     QFVNAPSDSAGGVAPECRIDGFAYNGTLRLMSPAMNMTGVNSVTLIFKHNYDKYASPS
                     PTIGVATKSSGAWNPVWTMVPSSDTGPEEIQVTINNSDLNKPNFQISFYVTGTIQNMN
                     HWYFDNILLMVPLELDGKISGITIPGTITSPQEVGGVFQNIGSTAITDFSVGFEAYNG
                     MIYDTSFTGLNLEMFDAFDFTFDQMWVQPFGTHNINMWINNVNGITDDNPGNDTVTKS
                     ITYIANILPRKVTFEEFTSSTCGPCASFNSGFVPWCESHPDIMLVKYQMNWPGAGDPY
                     YTAEGGTRRNYYGVSYVPDLFYNGARINASVSAVQTSYNQGLALTSYIDIASSFTITG
                     TTINITTNLLPWDNVGNVRVHNIVIEKVTTGNVSSNGETEFHHVMMDMIPDANGASVN
                     LQYATPVKLQYSVNLAGTNVEEYDDLLVAVLVQNQSTKEMLQSEYGEMNTAYSADARL
                     EMINLDGVPLEGFNPDIYEYDVILPEGTLFEPYLEAVLMDDG"
BASE COUNT         1498 a         1336 c         1388 g         1562 t
ORIGIN      
        1 gcagccggac tttaccccga atggttagca tggccgttga aatggtgcct tgacctcatg
       61 ctgggttcag tggaatggat ccaaagctgg cccggcgccg tcctccggcc gtttctgccg
      121 gatgcagcca ctactatttt ttcttatgct gcaatcctat tcctgtttat catgatttca
      181 acccggaaaa aaaagtggtt attgcctttg atgtcggcaa tcgccgctgt ttcggtcttg
      241 tttgcagtca accaatacaa ccggattgaa cgatgtgcac tgtatgtgta tcagcacaaa
      301 ggctctctcg ccattgatat gatgaaaaac aggcggaatt tgtttgtcac ggatggaaga
      361 atgcaaaagg attcgacccg tttacaaatg ctggttgagg ccaacaggat aaaaaacaga
      421 ataagtctga cagatacact gcagatcagc gagaaagggc agataggtcg tcaggagtac
      481 tggtattctc gtccttttct gaattacatg ggaaagaaga ttgtttttat ccatgacgaa
      541 tggctgcaag gaaatgctgg acaaagggtg caatgtgatc tggcgatcct atccggtcaa
      601 tccggattca atgatccggc tgttttaaac tgcgtgaagt tcgaaagggc catcctggct
      661 cctgatctgt cctattcccg ggaggagagg atagtcaaat ggctcaacga tgccggaatt
      721 ccttttcatt ctgtcaggaa agacggcgct ttcgtgatgg aatggagaag cagtgaatag
      781 tgaataatag ttgctaattg ctgtttattc agttattatc agttttctgg acgattgttt
      841 tccggagtgg cgcaggatga ccatgtagct gccggcggga agaggctcca gcgaggtgtg
      901 gatgaaatgg tctcccttct ccctgtagct gttatctaaa accgatactg ttgttccttt
      961 cagatcggcg atcaggattt ccacactgcc tgccgactca agggtgtagt cgatgcggag
     1021 caagtctgta gcaggattcg gattaatccg gaaatcaata ggctgatcac tggttttctc
     1081 atggatcccg atcccgttaa tgctgatctc aaggtcatcg atatacattc cctgctcttg
     1141 cacgaaaccg tcgctattaa accggaaccg gacaatgaca tccgattgcc cgatgtaatc
     1201 gtcgagcgaa taggtgagtt gtgtccagtc ttccatcaat cctgaatagt ttgccaatgg
     1261 ctcccaatcg aggccggcaa cggaaatttc caggttcatg aagtcccatc cggattcaat
     1321 ttcatacttg gtccagaacg acaattcggc cgaaaaggct cccgtcaggt cgatcggtcg
     1381 aagcgtactg gaaatctcaa ggttggcctc atatttttca tccgggcttt cggtaaggga
     1441 gttgctggag gaataataaa tgccttgttg caaaccccag gtaccttccc ggctccagaa
     1501 tagccccccg gtttcgaaat catcgaaaaa tggcagaata atgggcggca tgggcatgac
     1561 cgtaacgctg ttggaagggc ccgattcttc gcctgatcct tcgaatactg ccgtaacata
     1621 gtatgtgtag gaaaccatat tttccactcc ggtatcagta aacgaggttt cctccgtggt
     1681 ggcatacggt tcttcgcctc catcccggta aatattgtac gatgccgctt cggcaacagg
     1741 gtcccaggcc agttccacct gctgatcacc ggaaagtgcg gtcaggtctt caggacgggg
     1801 gaggaggtcc gacagtgaga caacattggc tattgctgct ttcacgaagg tcatatgctg
     1861 ttcgaagtta ttcacactgg gccctatcag atcgttggaa gtatggatgt acgggctgta
     1921 gttctgggaa tcttcaaagg gaaataagcc cgggtaaccg ttattgttga aggaggtatg
     1981 gtcgctgtcg ccaccggtaa gcattccgtt ggaagtttga aaatcaggca gataaattgc
     2041 acagatatct ttgtagaact ggcggagttc attggctgag gccggtgcga tgatatcggt
     2101 atggatggcg tcgccttcct ggaggtagcc ggccatatca atgttgaagt aacccaatat
     2161 gttcatttct tcgttggcgg cttctgttgc ccaggcgtcg cttccgtaaa gaccgtattc
     2221 ctcgccgctc caggctgcaa aaatgatcgt ccggtcgaac tggtactggc tgaggatcct
     2281 ggcagcttcc aggacaccgg cagtacccgt ggcattgtcg tctgcgcctg gctcattcga
     2341 accaccggcg taagaatcgt agtgggctcc tataatgacg tattcatcgg gataaacgtt
     2401 gccgggtaga gtggctatga cattatcgct ggcatttcct ccgggcatcc agaaatcgtg
     2461 gagttccgtt tccaggccgt acgactgtaa tttggccagg atccagttct gtgcctgtat
     2521 ccccccggcc ttataagcat tgcgggtgcc aaagtcctgc aatcgctgga caaaagcttc
     2581 cagcgtatcc atatcaacca ggtcgatcaa atccgcaatg taatcgcgca tctgaaggct
     2641 tccgggatga taagaaaaag attggtccgg gaaacgggcc gctttgttct ctatccggac
     2701 aattcccccg tggatggcag ggatcaattg ctttgtgttt tctccggatg tggagactgc
     2761 caaaaaatta cccgaatcat aaaggatctt tgcatatgca gcaacaagag cggtatattg
     2821 atcccggttt tctgcaggat aatacagaat gaaatagtgg acatcatcgg tcatccaggc
     2881 attattgtcg ataataaggc taccggccgg gctaccgtcg tcacttgatc cgatgatgaa
     2941 gtcatcatta taaaaatgaa tgacaaaagt gttttgatta aatgcagctt tagttctctc
     3001 tacagatcct gtattcagaa taaataattc cgaagaaaat gccgtcacac tcatggccag
     3061 cagaataacc gatattaaca gatttttcat tttattcata tttgacgcca aagatataaa
     3121 agtgttgggt gaaaaattga atgcgataaa aagatacaag ggtttattcg ctatttttga
     3181 taatactatt attgaatgcg atgggacaga cggaaaagcc ctggccgaaa tggcgaaatg
     3241 acctgcaccg gatcatattc ggcacagata catttgaggg caggctattc gatatcatac
     3301 tgatattctt tatttcagcg agcgtcctgg tcgtgttcct ggatagtatc ggttacatac
     3361 acaaacagta tggaagatgg ctatatggcc ttgaatggtt ttttaccatc gtattcacaa
     3421 tagaatatat attccgtatt ctgtctctta gaaagccatg gcaatatgtt tttagcttct
     3481 acggattgat agatttttta gctgtaatgc cgacctatat cagcctgatc ttcaccggcg
     3541 gtcagtatct cattgccatc agggcactcc gtttgctcag gattttccgg gtttttaagc
     3601 tgggtcggtt tctctgggca tccaggtacc tgatggtagc gatcagaaac agccggcaca
     3661 aaattgtggt attcctgtgg tttgtcatca ttattgtggt ggtcatgggt tctgtgatgt
     3721 acctggtgga agggcctgaa aatggtttta ccaacattcc cgacagcatt tattgggcta
     3781 tcgtaacact aacaacggtc ggttatggcg atatttcgcc ccagacaccc ctcggtaagg
     3841 ctattgcttc ggtcattatg ataatcggat acggtgtgat cgctgttccg actggtattt
     3901 ttaccgctga actggcacgg atgcactcat catcagacaa gacatgcaac cattgtcaaa
     3961 atgcgcagaa cgatcctgat gccaaatatt gtaaaaagtg tgggcagcct ttacaagacc
     4021 cttaaattta atcttttcat cctgttacgt ttaaaaaatc aaaattcaaa tcgacattat
     4081 attatatttg caacattaat tatcagcaat catcctcatc aaaaaaaaaa cactatgaaa
     4141 aagttctaca gcctgatcat tgctatttca ctggcatttc cggttttcag ccagacatac
     4201 ctgtcggaag atttcagcag tggcacgatg ccccctccgg gatggactat ttttgctttc
     4261 cagcagcaat ttgtcaatgc accatccgat agcgccgggg gtgtcgcccc tgaatgccgg
     4321 attgacggtt ttgcctataa cggaacactc agactgatgt cgccggctat gaatatgacc
     4381 ggagtaaatt cggtcaccct gattttcaag cataattatg ataaatacgc ttccccatca
     4441 cccacaattg gtgtggctac gaaatcaagc ggagcctgga atccggtctg gaccatggtg
     4501 ccctcttccg atacaggccc tgaagagatc caggtgacaa tcaataacag tgacctgaat
     4561 aaacccaatt ttcagatatc cttctatgtc accgggacta tacaaaacat gaatcactgg
     4621 tatttcgaca atatcctgct gatggttcca ttggaactcg acggcaagat atccggtatt
     4681 accataccgg gtaccatcac ctctccgcaa gaagtcggag gtgttttcca gaatatcgga
     4741 agtactgcga tcaccgactt cagcgtcgga tttgaagcct ataacgggat gatatatgat
     4801 acttcgttta ccggattgaa cctggaaatg tttgatgcgt ttgattttac atttgaccag
     4861 atgtgggttc agcctttcgg gacacacaac atcaacatgt ggatcaacaa tgtcaatggg
     4921 attaccgatg acaatcccgg aaacgacacg gtgacaaaat ccattaccta tattgccaat
     4981 attttgccgc gcaaagttac ctttgaagaa ttcaccagct cgacctgcgg cccgtgtgct
     5041 tcattcaaca gcggctttgt cccctggtgt gaaagccatc ccgatatcat gctggtcaaa
     5101 taccagatga actggcccgg cgccggcgac ccctattata cagcggaagg cggcacccgt
     5161 agaaattact acggcgtttc atatgtcccc gatttatttt ataacggcgc ccgtatcaat
     5221 gccagtgttt ctgctgtaca gacctcttat aaccaggggc ttgcgttgac ttcctatatc
     5281 gatatcgcat ccagttttac cattacgggc accacaatca acataacaac gaatctttta
     5341 ccctgggaca atgtcggaaa tgtcagggtg cataatattg tgatcgaaaa ggttactact
     5401 ggtaatgtca gttcaaacgg cgagaccgag tttcaccatg tgatgatgga catgatcccc
     5461 gatgccaacg gagccagtgt gaacctgcag tatgcaacac cggtcaaact ccagtacagt
     5521 gtaaaccttg ccggcaccaa tgtggaagag tatgatgacc ttctggtcgc tgttctcgtc
     5581 cagaatcagt cgaccaagga gatgctgcag tcggaatacg gtgagatgaa cactgcatac
     5641 tcagccgatg cccgcctgga gatgatcaac ctcgacggag ttccgctgga aggattcaat
     5701 cccgatattt atgaatacga tgtcattctt cctgaaggga cgctctttga gccttacctc
     5761 gaagctgtat taatggatga cggc
//
LOCUS       DAOPNH010000225         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16197_length_5736_cov_7.875022, whole genome shotgun sequence.
ACCESSION   DAOPNH010000225 DAOPNH010000000
VERSION     DAOPNH010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16197_length_5736_cov_7.875022"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKZ74_11150"
     CDS             complement(<1..184)
                     /locus_tag="PKZ74_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93353.1"
                     /translation="MKKLRLLILSLLLVNCFSNGQSVAPSGGAYQRANSLNYDAIVDT
                     AKLWSVAIEYMNIRYSL"
     gene            668..1351
                     /locus_tag="PKZ74_11155"
     CDS             668..1351
                     /locus_tag="PKZ74_11155"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPM93354.1"
                     /translation="MKLIGAGFGRSGTMSLKAALDELGYGPCYHMKTALPRFHHLHFF
                     IKAWKGETVNWKRFFRRYNSVVDWPTCSFYKELMQEYPDAKILLNVRDPEKWYDSMYE
                     TIWAIQPAFPFWFPGIVRRIHDEIIWNGNLQGAFNDRLMTVKIYREWIDEVRKTVPAE
                     RLLVYEVKEGWKPLCDFLGVPVPEKPFPHINERRSFRRLIRLLKVLNWLVPALIFMIF
                     VFTVLFLTD"
     gene            complement(1671..2981)
                     /locus_tag="PKZ74_11160"
     CDS             complement(1671..2981)
                     /locus_tag="PKZ74_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93355.1"
                     /translation="MFFLMLAPLFIHSQQDTTEQKQRKRLNFSGLPVVAYDADMGLQY
                     GLVANMYDYGDWKAYPEYRHMVKVEVSRFTKGSGINQVFYDSKYLIPGIRFTADLSYL
                     TEKGVDFYGFNGYQAIYRPEWEDDEDTSYITRMFYRHERKMFRFTSDFQGQIAGERLR
                     WLAGIGIMNFKTGTVDIDRLNKGKKESKILPDTITLYDKYVEWGIIPEDEKNGGNANF
                     IKAGLIFDTRDNEPNPMKGLWDELMLVYSPGFFFNDGNSFLKLIAIHRQYFTIVKKKV
                     NFGYRLGYQGTIAGHTPFYFQPYMLNSFSSITKTDGLGGAKNLRGILRNRVVGDGIVW
                     GNAEVRWRITDFVIAKQKFYVGLNVFGDVGMVVQKMDFNIPKTAETAEYFDWANSGDK
                     LHPSVGAGLRIGWNENFIIAVDYGFALDQRDGMRGLYIAFGNLF"
     gene            complement(3198..4535)
                     /locus_tag="PKZ74_11165"
     CDS             complement(3198..4535)
                     /locus_tag="PKZ74_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM93356.1"
                     /translation="MSRKTIGRFSFGFQIVLWIGGIFHRMYYRKFSVVNRERIPHNGP
                     VIFAANHQNALMDALAVIFSTRRQVVFLARADIFRKKYMARILYFLKILPVYRIRDGF
                     HAVDQNNEVFPEIIGVLKRNRPAGLFPEGNHLGEKRLRPLKKGAARLALQAEEANDFT
                     LGVTIVPVGLDYSNYYNAGSDLLVVFGNPIPALAYREEYLRNPAQAVSHMTTDLATEL
                     RKVMLDIRSEENYSVIHEAVKMYGPVERKRRKLRNNPYNLFLIKKELAENLMVKEKGL
                     ETLREELASYHAKLPALGIRDSQITSKIENRKSKIGTFIALFLLPIHLYGIVLNYIPY
                     GLPIFLARNIKDRHFLGSVRFGAGLLLFFTWYLLLFITSIFIFHNIFYAAVFMVSVSL
                     TGLCAFYNYRLMLKLRADWRWIFLKYRNRSEFEGLIKQRERIMKEIFRLLYRV"
     gene            complement(4542..5618)
                     /gene="meaB"
                     /locus_tag="PKZ74_11170"
     CDS             complement(4542..5618)
                     /gene="meaB"
                     /locus_tag="PKZ74_11170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPM93357.1"
                     /translation="MNEKELPEDSALRVNEGIDQPEAMNPEAARRFRDAIPKELDAEA
                     YVKGILEGSRPLLSKAITMVESALPRHQELAREIVNACVPHAGRSVRVGITGVPGVGK
                     STFIETLGKYLTAQGNRIAVLAIDPSSRRTKGSILGDKTRMEDLANDPNAYIRPSSSA
                     GTLGGVARNTRESVILCEAAGFNIIFVETVGVGQSETAVHSMVDFFLLLMLAGAGDEL
                     QGIKRGIMEMADGIVINKADGDNVRKAELAAREYKNALHLFPPPPSGWNPMVLTASAA
                     ENKGIAETWQMVLDYSRLAHESGYFDANRQEQALQAFTDTVEEAIRQQFYHSEIVISQ
                     IDTLRSDILAGKISPYEAARKLTQ"
     gene            5663..5735
                     /locus_tag="PKZ74_11175"
     tRNA            5663..5735
                     /locus_tag="PKZ74_11175"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5696..5698,aa:Lys,seq:ctt)
BASE COUNT         1394 a         1457 c         1413 g         1472 t
ORIGIN      
        1 ataatgaata tcgaatattc atatattcta tggcaacact ccataatttt gccgtatcga
       61 caatggcatc ataattcaag ctatttgctc tctggtaggc cccacctgat ggtgcaactg
      121 actgaccatt actaaaacag tttactaaca acaaggagag gatcaataat ctcaattttt
      181 tcataatatt tcggtattta ttgcgctttc ataccataaa accaaatcag aataatagct
      241 tacattaata agggtgagag ggaaataaac ctggttttca gaatttggat taacgtttca
      301 aatatagcgc aaaattcagt agatgtcaag atcttttaat caaatcttca taatttagat
      361 aagttctata taattcttca ttattggttt taagtatcag aaagtcttat gaatggctaa
      421 ttctcaaaag ttttccttac aactccatgc cggtattaat atgttggctt gaaaatcaat
      481 tttgttactt tgcagatgaa aagtgaagcg tgaagcgtga agggtgaaat tggaaaatag
      541 aaatttggaa ttggttccag gtttcgcggg aaagacgcga aacctggaac actagaactc
      601 tggaacactg gaacactgga acactggaac ccggaacccc ttacaaccct tccaacccct
      661 tgaacccatg aaactcatcg gcgcgggctt cggcagatcg ggaaccatgt cgctcaaggc
      721 ggcgctggat gagctgggct atggcccctg ctaccatatg aagaccgccc tgccgcgatt
      781 ccatcatctt catttcttta taaaagcctg gaaaggggag acggtcaact ggaagagatt
      841 tttcagaaga tataattccg ttgtcgactg gcccacctgc tcattttaca aggagctgat
      901 gcaggaatat cccgatgcga agatcctgct gaatgtacgc gatccggaga aatggtacga
      961 cagcatgtac gaaacgatct gggccatcca gccggctttc ccgttctggt ttccgggcat
     1021 cgtcaggagg atccacgacg agatcatatg gaacggcaac ctgcagggcg ctttcaacga
     1081 ccgcctgatg accgtgaaga tctaccggga gtggatcgat gaagtgagga aaacggttcc
     1141 ggcagagcgt ttgctggttt atgaagtgaa ggaaggttgg aaacccttat gcgatttcct
     1201 cggtgtgccg gtccccgaaa aacccttccc gcatatcaac gagcgcagga gcttccggag
     1261 gctgatccgg ctgctgaagg tgctgaactg gctggtgcct gctttgatct ttatgatatt
     1321 cgttttcaca gttttatttc tcacagatta atttctcaca gatttcacag attgaaagca
     1381 caaaatccac agaatgggac tgcaggactg taagacttta ggactgtagg gctgtaggaa
     1441 tgtgcaaatt tccagaagag agtttcttta gagtctgaac agcctctttt ttgtagtccg
     1501 acgactgaag ccgtcggttc cagagggatt ctcttccgac gggtaaatcc gtcggttcta
     1561 tcgttcctgg aaccaacggc tttagtcgtt ggagcacgaa taccaaaagg taagaactaa
     1621 tctgtggatt ctgtgctttc aatctgtgaa atctgtgaga aacaaaaacc ctagaacaaa
     1681 ttcccgaaag caatgtacag ccctctcatc ccgtcccttt gatccagcgc aaaaccataa
     1741 tcgacggcaa tgatgaaatt ctcgttccag ccgatgcgca ggccggcgcc gacactggga
     1801 tgcagcttgt cgccgctgtt ggcccaatcg aaatattctg ctgtttcagc cgttttcggg
     1861 atattgaaat ccatcttctg taccaccatt cccacgtcgc cgaaaacatt cagcccgacg
     1921 tagaattttt gcttggcgat gacaaagtcg gtaatccgcc agcgcacctc ggcattgccc
     1981 caaacaatac catctccgac cacccggttg cgcaaaattc cgcgcaggtt cttcgccccg
     2041 cccaggccgt cggtcttggt aatagatgag aaagaattga gcatgtaggg ctggaaatag
     2101 aacggcgtat gaccggcaat cgttccctgg tagcccagcc ggtagccgaa attcaccttc
     2161 ttcttaacga tggtgaaata ctgccggtgt atggcgatca gcttcaggaa tgaattgccg
     2221 tcgttaaaga aaaatccggg agagtaaacc agcatcagct catcccacag gcctttcatc
     2281 gggttgggct cgttgtcgcg cgtgtcgaag atcagcccgg ccttgataaa attggcatta
     2341 ccgccatttt tctcatcctc gggaataatc ccccattcaa catacttatc gtaaagggtg
     2401 atcgtatccg gaaggatctt gctctctttt ttacccttgt tcaacctgtc gatatccacc
     2461 gttcccgttt taaaattcat gatcccgata ccggccagcc agcggagcct ttcaccggct
     2521 atttgtccct ggaaatcact cgtgaagcgg aacatcttcc gttcatgccg gtaaaacatc
     2581 cgggtgatat acgatgtgtc ctcatcatcc tcccattccg gccggtatat cgcctggtat
     2641 ccgttaaatc catagaaatc aacgcctttc tccgtcagat aactcaggtc ggccgtgaac
     2701 cggataccgg gaataaggta tttggaatca taaaagacct ggttaatgcc acttcccttc
     2761 gtgaaccgcg atacctccac cttaaccata tgccggtatt cggggtaggc tttccagtcg
     2821 ccgtagtcgt acatgttggc cacaagtccg tattgcagcc ccatgtcggc atcgtaggcc
     2881 acgaccggca acccggagaa attcagtcgt ttgcgctgtt tctgctctgt cgtgtcctgc
     2941 tgggaatgga tgaaaagcgg cgccaacatt aaaaaaaaca aaaatccgtt ggttaatctt
     3001 tttatcatca tattatttcc tccctcagtg cttatctgtg ttaatcggtg taatctgtgg
     3061 taaaattata acttacacca cagattacac agaactttcg gttttattta tactcattct
     3121 ttgcaggttt gcggcagcaa agctgcttag aatgagttat accgagaaga acaactgtat
     3181 caaattcgca aacctgttta gactcggtat aaaagtctga atatttcttt cataattctt
     3241 tccctttgct ttatcaaccc ctcaaattca ctcctgttgc ggtatttcag aaagatccac
     3301 cgccagtcgg ctctcagttt cagcatcagg cggtaattgt agaatgcgca caggccggtc
     3361 aaagataccg aaaccataaa aacagccgca taaaaaatat tgtgaaaaat gaatatagag
     3421 gtgataaaaa gcaggagata ccaggtaaag aataacagga gcccggcgcc gaacctcacc
     3481 gagccgagga aatgccggtc tttgatattc cgcgccagga atatgggcag gccgtaaggg
     3541 atgtaattca ggactatgcc ataaaggtgg atggggagca ggaacagggc gataaaagtg
     3601 ccgattttcg attttcgatt ttcgattttc gaagtaattt gggaatcgcg gatgccgagg
     3661 gccgggagtt ttgcgtggta agacgccagt tcttcgcgga gggtttccag ccctttttct
     3721 ttaaccataa gattctcagc caattccttc tttattaaga aaagattata cggattgttc
     3781 ctgagttttc tgcgcttccg ttcaaccggc ccgtacattt tcactgcctc atggataacg
     3841 ctgtagttct cttccgaacg gatatccagc atcactttcc ggagctccgt tgccaggtcg
     3901 gtggtcatat ggctgactgc ctgggcaggg ttccgtaggt attcctcgcg gtaggccaat
     3961 gccgggatgg gattgccgaa cactaccagc aggtcgctcc cggcgttgta gtagttggaa
     4021 taatcgagcc cgacagggac gatggtcacc cccagcgtaa aatcattggc ctcctccgct
     4081 tgcagcgcca gccgtgccgc tccttttttc aacgggcgca accgtttctc ccccaggtgg
     4141 ttcccttcgg ggaacaggcc tgccggccgg tttctcttca gcacgccgat gatctcggga
     4201 aatacttcat tgttctgatc gacggcgtga aaaccatcgc ggatgcggta aacaggtaag
     4261 atcttcagaa agtagagaat ccgggccatg tattttttac ggaagatgtc ggcccgggcc
     4321 aggaacacca cctggcggcg ggtggaaaag attacagcca gcgcatccat cagcgcattc
     4381 tggtgattgg cggcaaaaat gaccggaccg ttatgcggaa tcctttccct gttgaccacg
     4441 gaaaacttcc ggtaatacat cctgtggaaa atgcccccga tccagaggac gatctggaac
     4501 ccgaaggaga atctgccgat ggttttcctg gacatagaaa attattgagt cagctttctc
     4561 gccgcctcat acggactgat ctttccggca aggatgtcgc tgcggagggt gtcgatctgt
     4621 gaaatcacta tttcactgtg atagaactgc tgccggatcg cctcttcgac ggtatctgta
     4681 aaggcctgca acgcctgttc ctggcggttg gcgtcgaagt aacccgactc atgggccaac
     4741 cggctgtaat ctaaaaccat ctgccaggtt tcagcgatgc ccttgttttc ggcggccgag
     4801 gcggtcagca ccatcgggtt ccatcccgac ggcgggggag ggaagaggtg gagggcattc
     4861 ttatattccc gcgctgccag ttcggctttc ctgacattat cgccatcggc tttattgatg
     4921 acgatgccgt cggccatctc catgatgccc cgcttgatcc cctggagctc gtcgccggcg
     4981 ccggccagca tcagcagcag gaagaaatcg accatgctgt ggacggccgt ttccgattgc
     5041 cccacgccga ccgtctcgac gaagatgatg ttaaatcctg ctgcttcgca gaggatgacc
     5101 gattcgcgcg tattgcgcgc cacgccgccc agcgttccgg cagaggagga gggtcggatg
     5161 taggcgttag ggtcgttggc caggtcttcc atccgggtct tgtcgcccag gatgcttcct
     5221 ttggtgcggc ggctgctcgg gtcgatcgcc agtacggcga tcctgttgcc ctgtgccgta
     5281 agatatttcc cgagcgtttc gatgaacgtg ctctttccca ctcccggcac ccccgtaatg
     5341 cccacgcgga ccgatctccc ggcatgcggt acgcaggcgt tgacgatctc ccgggccagc
     5401 tcctggtggc gtggcagggc gctttcgacc atcgtgatgg ctttgctgag gagcgggcgg
     5461 ctgccttcca ggatcccttt cacatatgct tcggcgtcaa gctctttcgg gatggcgtcg
     5521 cggaatcggc gtgccgcctc cgggttcatc gcttccggct ggtcgatccc ttcattcacc
     5581 cgcagagcgg aatcttccgg tagttctttt tcattcatta cgacaaatgt aaagaaaatt
     5641 atgtactttt gccgccgcaa aactctccgt agctcagttg gtagagcaat tgactcttaa
     5701 tcaatgggtc ccaggttcga atcctggcgg ggagac
//
LOCUS       DAOPNH010000226         5734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16207_length_5734_cov_6.943124, whole genome shotgun sequence.
ACCESSION   DAOPNH010000226 DAOPNH010000000
VERSION     DAOPNH010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16207_length_5734_cov_6.943124"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..953)
                     /locus_tag="PKZ74_11180"
     CDS             complement(45..953)
                     /locus_tag="PKZ74_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93358.1"
                     /translation="MKVIGVFFLLLISGSQFLHAQCCAPGNPVSGSEYVGALPRNTLR
                     TILFYKHSYSDTYYEGSERSDYQGTIAGYDFIGGAFSYGILKRLTAEAELGFYIDKYQ
                     DSDVLDRFTTYGLTNAVISAKYALLKSDKKAELTIGAGVKIPVSQKVFADEYGIPYPQ
                     EIQPSSGAYGFVGQVFFFKGFREDKWRLVIHGRYERNGYNKAEYRFGDAILASVFIGR
                     NFTKKWSATLQLRNEYRMEDWQGQTRYLVTGGDILFISPQISHTFKHRITVSVMGDVP
                     VFRHYNGVQLGPKYAFGVSLVKDFCF"
     gene            complement(978..1748)
                     /locus_tag="PKZ74_11185"
     CDS             complement(978..1748)
                     /locus_tag="PKZ74_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93359.1"
                     /translation="MANRILQFIIGLIFLSGEWAVSQPVAMDFSLDDCGGTNHHLFSE
                     LDAGKVVILDFVMLNCAPCIVATNALSEIVSGYEASHPGRVRIYSFGFLNSYTCEQMT
                     AWKNSNNYEHSVFSGGEEQVAYYGGMGMPTIVVLGTNEHNVLFKTVGYSATLDDKIRT
                     AIDSALQYSPTGTGDMPADESRFRAYPTFFSDRITVETPSHGTIELTDVLGRITGVYQ
                     VQDADVACINTDNFLPGLYFLRWSDDHGREYVVKMFKK"
     gene            1990..2646
                     /locus_tag="PKZ74_11190"
     CDS             1990..2646
                     /locus_tag="PKZ74_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93360.1"
                     /translation="MKKKEKVKLEKALAKAEKKLKKTLKKAQKAIKEAKKLGLELTGN
                     TLIKPSKQVKAKKITSDPKPVEIPEIKSETKAPARRGRPKKTEAVALPKPKKVRKTRV
                     VAKKEETPVVQEPVQVKETVKKPTRRAPKAKAPAAEIKEVKPKRVPKARIPKAEKKAE
                     APKAETEPKKPKARKPGTAKKPAAPKTKTTVKKSRTLKPKAAPEAPPELPEAPAAEMV
                     "
     gene            complement(2662..4158)
                     /locus_tag="PKZ74_11195"
     CDS             complement(2662..4158)
                     /locus_tag="PKZ74_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM93361.1"
                     /translation="MPTKVSIIGAGIAGLSAGCYLQKSGFDTEIFEAHNLPGGLCTSW
                     NKDGYLIDGCLHWLMGTHPSSPVYRLWNEIIDMDSLPVFVYDEFFRIQDASGKEIVAY
                     ADLERLREELKSKAPEDRALVDEFVNAARKLGRMKMRMDKAPELYSARDKLGETLSYL
                     PYLNLLIRYSKMTIHDFAAKCKNPLLRNFFEYSFTSEMPVLFIMMTFSWLDGKNAGYP
                     VGGSLKFAQLFEKKYLEQGGKIRYNSRVTGIITEKNGKRDRAAGIVTEKGEESRADIV
                     ISCADGYSTIFGMLGGRYADKRIQHYYESFQVFPSFFLVSLGVSRTFGNVPSRVIFPL
                     DKPFRLDPEKVTDHMNYRISSYDPTLAPEGKTLIMCLAKTFNSRYWMDLRKNDYRKYS
                     EEKQRIADFYIDQLDRKLGGIRENLEMTDIATPATLHRYTHNWKGSVEGWMVTRETGF
                     DSLPKVLPGLDDFYMAGQWVEPGGGVPAVFFSGRNLAQIIAGKYGQGF"
     gene            complement(4209..4751)
                     /locus_tag="PKZ74_11200"
     CDS             complement(4209..4751)
                     /locus_tag="PKZ74_11200"
                     /inference="COORDINATES: protein motif:HMM:NF033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HPM93362.1"
                     /translation="MTEKNLTSNGPSDPEDDYIKSWLRSLTILHRGHWDAARYYDKVN
                     LGLGLATAIAAAISGTTAFTQLQSQAEEGTLNTWLQVGIGIFALLAASLGAAQAFMRP
                     SELSARHKLAGQKYGKLRREIELAFYLGLPDQQEKKETFLTEFRLKWEAADEESLPVP
                     EKIYKSVEADYKNRTPRINV"
     gene            complement(4932..5414)
                     /locus_tag="PKZ74_11205"
     CDS             complement(4932..5414)
                     /locus_tag="PKZ74_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93363.1"
                     /translation="MKKIIQVATVVLIFLVAASGASAQEWSKEQTEVWKVVQDTWKGW
                     SSGDISGVTGNIHEKYQGWSDDSPLPIGKQTMIDWFNSMKDAVSFNYYDIQPARITVL
                     NSAAVVDYYYYFNATWNMGEEKKTEEMKGKIAEFYVKDGGKWLLLGDLMVHEEVEKDD
                     "
BASE COUNT         1602 a         1485 c         1225 g         1422 t
ORIGIN      
        1 gacccagccg aaagccccgg caaaaaagaa gagttaaagg ttacttaaaa acagaaatct
       61 tttaccaggc ttaccccgaa agcatacttc gggcctagct ggacgccatt gtagtgcctg
      121 aaaacaggca catctcccat aaccgatacc gtgatccggt gtttgaaggt gtggcttatt
      181 tggggagata tgaacaagat atccccaccg gttaccaggt aacgtgtctg cccctgccag
      241 tcctccatcc ggtattcatt cctcaattgc agtgtggccg accatttctt cgtaaaattt
      301 ctgccgatga aaacggaagc gagaatcgca tcaccaaaac ggtactcggc tttgttataa
      361 ccattgcgtt cgtacctgcc gtggataacc agtctccatt tatcttcgcg gaatccttta
      421 aagaaaaata cctgtccgac aaatccgtaa gcccccgacg aaggctgtat ctcctggggg
      481 taaggaatac catattcatc cgcaaatact ttttgcgaaa caggaatctt gacgcctgcc
      541 ccaatcgtta gctcagcctt cttatccgat ttcaacaaag catatttagc agaaataacc
      601 gcatttgtca gtccgtacgt tgtgaaccgg tccagcacat cgctgtcctg gtatttatcg
      661 atgtaaaaac ccagctccgc ctctgcagtc agccttttca gaataccata ggaaaaagcc
      721 ccgccgataa aatcatatcc ggcaattgtc ccctggtaat ccgaccgctc cgagccttca
      781 taataggtgt cggaataact gtgtttgtaa aacagaatag tacgtaaagt attccgggga
      841 agggcaccga catattcact gcctgaaacg ggatttcccg gtgcacaaca ttgggcatgc
      901 aggaactgcg atcctgaaat cagcaacaga aaaaaaactc caatgacttt caaggatttg
      961 ctattcaaat gaatgagtta ctttttgaac atctttacca cgtactcccg tccatggtca
     1021 tctgaccatc ttaaaaaata caatcccggc aggaaattat cggtattgat gcaagccaca
     1081 tcagcatcct gcacctggta aactccggtg atcctgccca gaacatccgt taattcaata
     1141 gttccatgag atggagtctc aaccgtgatc ctgtccgaaa agaacgtggg atacgcccgg
     1201 aaccgggatt cgtctgccgg catgtcgcct gttccggtcg gactgtattg cagagccgaa
     1261 tcgatggctg tccgtatctt gtcatccagc gtggccgaat agcctacggt cttaaaaaga
     1321 acattatgct cattcgtacc caaaacgaca atagtcggca tacccattcc cccgtaataa
     1381 gctacctgct cttcgccgcc gctgaaaacg gaatgctcat aattattgct gttcttccag
     1441 gcggtcatct gttcacaggt gtatgaattc agaaatccga aggaatagat acgcacgcgg
     1501 ccagggtgtg aggcctcata tccggaaaca atctccgata aagcattcgt ggcaacaata
     1561 cacggcgcgc aattcagcat cacaaaatca aggataacta ctttgccggc atccaactca
     1621 ctgaataaat ggtgatttgt tcccccgcaa tcatccagtg aaaaatccat tgccaccggc
     1681 tgggaaacgg cccattcgcc agaaagaaag atcaggccga taataaattg taaaatccta
     1741 tttgccatta ttttatcctt catgaacatt aatagtgcaa atatattcaa tttattatat
     1801 attttgatga aataccattt tcattaacgg tgttttaaca attcaatacc ataaatattc
     1861 cagtagtatc tggtttataa tataatataa tatttaatct atattgatta attattgcta
     1921 catttgtggc ctcttctaag taaacaggcg gatattactt tattatttat tttaaactct
     1981 gaaatcaaaa tgaaaaaaaa ggaaaaggta aaactggaaa aagccttggc taaagcggaa
     2041 aagaaattaa aaaaaacact gaagaaagca caaaaagcca ttaaagaagc caaaaagctt
     2101 gggttggaat taaccggcaa cacgttgata aaacccagca aacaggtaaa agcgaaaaaa
     2161 atcacttctg atcccaagcc ggtggagatt cctgaaatca aatccgaaac aaaagccccg
     2221 gcccgccgcg gacgtcccaa aaagaccgaa gctgtggcac tgccaaaacc aaaaaaagtc
     2281 cggaaaacca gggttgtcgc taaaaaagaa gaaacacccg tagttcagga accggtacaa
     2341 gtgaaagaaa ccgttaaaaa acctacacgc agggctccaa aagccaaagc accggcagcg
     2401 gaaattaaag aagtaaagcc gaagcgcgta ccgaaagcca ggattccgaa agcggagaaa
     2461 aaagccgaag cgccaaaagc tgaaaccgag cccaaaaagc cgaaagccag gaagcccggg
     2521 acagctaaaa agcccgcagc gcctaaaacg aaaacaacgg tgaaaaaatc caggaccctc
     2581 aagcccaaag ccgcaccgga agcgcccccc gaacttcccg aggctccggc tgcggaaatg
     2641 gtataaaatc cgttttgaga attaaaatcc ctgcccgtat tttccggcaa tgatctgcgc
     2701 aaggttgcgt ccggagaaga aaacagcagg gacgccgcca ccgggctcca cccattggcc
     2761 ggccatgtag aaatcatcca gccccggaag gactttcggc aaactgtcga accccgtttc
     2821 ccgtgtcacc atccaccctt ctacacttcc cttccagttg tgtgtataac ggtggagtgt
     2881 tgcaggagtg gcaatatcgg tcatttccag gttctccctg atccctccga gcttccggtc
     2941 gagctgatcg atataaaaat ctgcgatccg ttgcttttct tccgaatact tccggtaatc
     3001 attcttgcgc aaatccatcc agtagcggct gttgaaggtc ttggccaggc acatgatcag
     3061 cgtcttgcct tccggcgcca gcgtgggatc ataactggaa atccggtaat tcatatggtc
     3121 tgtcaccttt tcaggatcca accggaaagg tttatccaat ggaaatatta cccgcgacgg
     3181 aacattcccg aaagtccgcg atacccccag cgaaaccagg aaaaaagagg gaaatacctg
     3241 gaaactttca taatagtgct gaatcctttt atctgcatat cgccctccca gcattccaaa
     3301 gatggtggaa tacccgtcgg cgcaggagat gacgatatca gcccgcgact cctccccttt
     3361 ttcggtcaca ataccggccg cccggtcgcg cttcccgttc ttttcggtga tgatcccggt
     3421 caccctgctg ttgtacctga tcttacctcc ttgttccagg tacttttttt cgaagagctg
     3481 tgcgaatttc agcgaaccgc cgaccgggta gccggcgttc ttcccgtcga gccaggaaaa
     3541 ggtcatcatg atgaaaagca ccggcatctc tgatgtaaag gagtattcga agaaattccg
     3601 cagcaaagga tttttgcact tggccgcaaa atcgtgaatc gtcatcttgc tgtagcggat
     3661 cagtaaattc aggtaaggca ggtagctgag cgtttcgcca agcttgtccc tggcggaata
     3721 gagttccggg gctttgtcca tccgcatttt catcctgccc agtttccgcg ccgcattgac
     3781 aaactcatcg accaaagccc ggtcttccgg cgctttcgat ttgagttctt ctctaaggcg
     3841 ctccaaatcg gcataagcca caatctcttt gcccgaagca tcctgtatcc ggaagaattc
     3901 atcgtacaca aaaaccggca gcgaatccat atcgatgatc tcattccaga gccggtatac
     3961 cgggctggaa ggatgggtgc ccatcagcca gtggaggcag ccgtcgatca ggtaaccatc
     4021 tttgttccac gatgtgcaca ggcctccggg caggttgtgc gcctcaaaga tctccgtgtc
     4081 aaaaccgctc ttttgcaggt aacagccggc ggataagccg gctatgcccg ccccgataat
     4141 gcttactttt gtcggcaaat tgaattgatt ttccctcaaa aataggtgat ttattcacac
     4201 cggatctttc agacattaat ccttggcgtt ctgttcttat aatcggcttc caccgattta
     4261 taaatctttt ccgggaccgg caatgattct tcatcggccg cttcccattt caaccgaaac
     4321 tcggtcagaa acgtctcctt tttctcctgt tgatcgggca aacccaggta aaaagccagt
     4381 tcgatctcgc gccggagttt gccgtacttc tggccggcga gcttatgccg ggccgacagc
     4441 tccgacggtc gcataaaagc ctgcgcagcg ccgagggagg cagccagcaa cgcaaatatc
     4501 ccgataccga cctggagcca ggtgttcaga gtgccctctt cggcctggct ctgcagctgg
     4561 gtgaacgccg ttgttccgga gatagcggcg gcaatggcgg tggccaggcc cagtccgagg
     4621 ttcactttat cgtaataccg ggcagcatcc cagtgcccgc ggtgcaggat cgttagtgaa
     4681 cgcagccagc ttttaatgta gtcatcttcg ggatcggaag ggccgtttga ggtaagattt
     4741 ttttctgtca tcggattaaa tattaattat agaaaattat tgcccaaaac agggtataaa
     4801 tataataatt taatctgatt accagtcata tagttgttga atagtttttt atttccggtt
     4861 tccaatgcac tatgcacgaa ggcaaaaggt tatccttccg aaatcgtgca tagcgcctcg
     4921 gaaatcagaa atcaatcgtc cttctcaact tcctcatgta ccatcaaatc acccagcagt
     4981 agccacttcc ccccatcttt tacataaaac tcggcgatct ttcctttcat ctcctccgtt
     5041 tttttctcct cacccatatt ccatgtggcg ttgaaataat agtaataatc caccactgcc
     5101 gctgaattca ataccgtgat ccgtgccggc tgaatgtcgt aatagttgaa tgatacagcg
     5161 tctttcatac tgttgaacca gtcgatcatc gtctgttttc cgatgggcag cggcgaatcg
     5221 tcgctccacc cctggtactt ttcatggatg ttccccgtta cccctgatat atcaccggaa
     5281 ctccagcctt tccacgtgtc ctggaccact ttccatactt cggtctgttc tttcgaccat
     5341 tcctgggcgc tggcgcctga cgcagcgacc aaaaagatca agaccactgt tgcaacctga
     5401 ataatttttt tcatgtgttt tgattttagg ttttgttatt gaattgagaa actttaaagc
     5461 aaatccaaaa ttattcaaat aatcctggat atcaaatatt taactattct ttaacaaaat
     5521 cttaagcact ttacaaaagg tagctgcatg aaaatacctc aaaacacatg taccaccttt
     5581 gagacagatt gaccgctgag ggaaacagcc cggatccgat agatcccgtt gcatcatccg
     5641 ggtaattgcc ctctcctaca tatgactccg gtttaaaata ctgatgcaag gttaatgaat
     5701 aacgattccc tgttgcaaat tgaccctgag ttgc
//
LOCUS       DAOPNH010000227         5721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16261_length_5721_cov_5.549418, whole genome shotgun sequence.
ACCESSION   DAOPNH010000227 DAOPNH010000000
VERSION     DAOPNH010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16261_length_5721_cov_5.549418"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKZ74_11210"
     CDS             complement(<1..301)
                     /locus_tag="PKZ74_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HPM93364.1"
                     /translation="MKNKFTLIFALCLWSVATLGGGYQVRLQGHKQTGFGLIGTPFAR
                     DASSIFYNPGSLAFYEGKFSISAGASGIFSNVLFQMQGTDYQARTDNPTGTPYYGY"
     gene            complement(380..1396)
                     /locus_tag="PKZ74_11215"
     CDS             complement(380..1396)
                     /locus_tag="PKZ74_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPM93365.1"
                     /translation="MKKVLGLGNALVDIMTQLDHDDYLSAFELPKGSMILVDARRSHK
                     IYDDTIHLQKTVRSGGSAANTIHGLANLGVETGFIGKIGRDEMGKVFHDDMITAGINT
                     HLSQDDTATGRAIALISPDTERTFATYLGAAVELNRNDLSRETFSQYDFFHIEGYIVQ
                     NHSLLESALKMARDCRMTISLDLASYNVVAENRDFLEEMIRKYVDITFANEDEARYLT
                     GLEPEEALEVLAGMCEIAVVKVGREGSIVGSRQSAVGSRQSAVGSRQIKIGVIPVKSV
                     DTTGAGDLYASGFLYGLANGYDLEKCGRIGAVAAGKVIEMIGPKLNDQKWREVRDFIR
                     FNIL"
     gene            complement(1492..2439)
                     /locus_tag="PKZ74_11220"
     CDS             complement(1492..2439)
                     /locus_tag="PKZ74_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93366.1"
                     /translation="MKNRTISLPLAILLMVLIAAGGIIAFTVERNDKKQLLAEKEGIM
                     KANEEKVLAVYNRIEKNLASIREKESMISQGFAGPEYNSDLAPEDQIQNEINFIKNLI
                     DENNKLIASLNDQIDEKDSRISGYQKSIKEYQAKVTVYQQQLDQLIAEKEVLRRDLDY
                     TIQAKDQLATQVNLLDSAVDRQNSIIAGQQQELTEKEKALHTAYYRIDTYKTLRDQNI
                     LEKEGGFLGINRVTTLADQVDNNLFSRIDTREVVRIPVAAKQWEIVTGQDPASYDVVF
                     ESKNNVEWINITDPEKFWGKSKYLVVVVRENSSELASNR"
     gene            complement(2639..3982)
                     /locus_tag="PKZ74_11225"
     CDS             complement(2639..3982)
                     /locus_tag="PKZ74_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM93367.1"
                     /translation="MQKEHFRIFVVEDDEWYNRFLVHHLSMNPEYEVKGFSSGTDLLK
                     SLHETPDVITLDYRLPDIKGNDLLGKIKLHDESIEIIVISGQEDVQTAVDILKEGAFD
                     YLVKSENIQARLHNTIQKIRDNAGLRKRIQVLEKEVVTRYGFEKAIIGNSPAMAKVLE
                     IMHKGAESNITVTITGETGTGKEVIAKAIHYNSARKNKPFVAVNMSAIPAELFESEFF
                     GHEKGAFTGALARRKGKFEEAEGGTIFLDEITDTDISFQPKLLRALQEREIIRVGSNE
                     PVRFDCRLIVASNRNLQQEVKKGTLRQDLFYRIFGLPIELPPLRERGKDILLLSKYFI
                     ENFCKENNLPVKEISAEAFDRLMQYPWPGNVRELRSVIELSVIMSSGSSIKASDILFS
                     QADILYEIIGEEMSMKQYNKRILDYYLNKYQRNMPVIASKLGISMATIYRMLKDQ"
     gene            complement(3986..>5721)
                     /locus_tag="PKZ74_11230"
     CDS             complement(3986..>5721)
                     /locus_tag="PKZ74_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93368.1"
                     /translation="TTYDRVITDKLRAMISGLDQQAAKYVKEHTDRVEKEASKVRFII
                     LVFGIASAILLILAGTVIFLYIRRNNEYRKILKKAREDAEALAQARQQFLANMSHEIR
                     TPMNIISGYLDQILKMPLEPELNAQLGIVRKSSDYLLELLNNLLDLSRIQAGKMELIH
                     TEFNPAEITREMHHWFSRAAEEKNLKFMAEIDPKVSLKLVGDPVRLRQILFNLISNAI
                     KYTDQGSVTIKARSGPADADTFRAVFEVTDTGAGIAQDEMERIFSEFTRGAVALGKSE
                     GSGLGLNITSKLVELMDGKLEVTSTPGKGSTFRVEIPFGLATGSSEPAPSGYLPDPAR
                     LKGMNILVADDDTYNRSLLLMILDKYHCRVIECSNGQEAIQAAHDNDPDLVILDMRMP
                     GKSGPDAAIEMNRIFAGRGKTVPIIALSAAITEEDRERLKPAGITHWMAKPFEEQRLI
                     SAILDLTRPSREVCRVDLEALRKSCEGNVAFFKEMVYLFLENTQTGLKEMEEKIDNGS
                     WDDAAAVAHRISASCRHLRADKLYSLLKEAEEHSVEGKKKFLKEIVSKAKEEFERIQK
                     SILSEPEFKTP"
BASE COUNT         1230 a         1502 c         1363 g         1626 t
ORIGIN      
        1 catagccgta gtatggcgtg cccgtcgggt tgtcggtccg tgcctggtaa tccgttccct
       61 gcatctggaa cagtacattg ctgaagatgc cgcttgcgcc ggccgatatg ctgaacttgc
      121 cttcataaaa cgccagcgaa ccggggttgt agaagatgga gctcgcatcg cgggcgaaag
      181 gggtgccgat caggccgaag cccgtttgct tgtgtccctg caggcgtacc tggtagcctc
      241 ctccgagggt ggcaaccgac cataggcata atgcaaagat cagagtgaat ttgtttttca
      301 taaggtgagt tattggttag aaaaagggcg caatttacat atttattcaa tcttataaca
      361 aaatatgtaa aaaattgaat tacagaatat tgaaacggat aaaatcgcgg acctcccgcc
      421 atttctgatc attcagcttg ggcccgatca tttcgatcac ttttccggcc gccaccgctc
      481 cgatccgtcc gcatttttcc aggtcgtacc cattagccag tccgtaaagg aacccggagg
      541 catacagatc ccccgccccg gtggtgtcga cacttttcac cggtataacc ccaattttga
      601 tctgcctgct gccgactgcc gactgcctgc tgccgactgc cgactgccga ctgccgacta
      661 tcgacccctc tctccccacc ttcaccactg ctatctcaca catccccgcc agcacttcca
      721 gcgcttcttc cggttcgagg ccggtcaggt atcgggcctc gtcttcgttg gcaaaggtga
      781 tatcgacata cttacggatc atctcttcca ggaagtcgcg gttttcggcc accacgttgt
      841 agctggccag atcgagcgaa atagtcatcc ggcagtcgcg ggccattttc agggcgcttt
      901 ccaggagcga atggttctgg acgatatagc cttcgatgtg gaagaaatca tattgcgaga
      961 acgtttcccg ggagaggtcg ttgcggttga gctcaacggc agcgccgagg taagtggcaa
     1021 aggtccgttc agtatccggg ctgatcaggg cgatggcgcg gccggtggcc gtgtcgtcct
     1081 ggctgaggtg ggtgttgatg cccgctgtga tcatatcgtc gtggaacacc tttcccatct
     1141 catcccggcc gatcttaccg ataaaacccg tctccacccc caggttggcc agtccgtgaa
     1201 tagtattggc ggctgagcct ccggagcgga cggttttttg caaatggatg gtgtcatcgt
     1261 aaatcttgtg agagcggcgc gcatccacca ggatcatgct ccctttcggc agttcgaatg
     1321 cgctgaggta atcgtcgtgg tcgagctggg tcatgatgtc gacaagcgcg ttgccgaggc
     1381 cgaggacttt cttcattgcg gcagagggtt tagagttttg ctgccggcaa agataatgga
     1441 taaaaaaaag cgggtgacgg gtctccccgc cacccgggtc gatatataga attacctgtt
     1501 tgaagccagt tcactgctgt tctcacgaac gacaacaacc aggtacttgc tcttacccca
     1561 gaatttctcg ggatcggtga tatttatcca ctccacatta tttttacttt caaaaacaac
     1621 gtcgtacgat gcaggatcct gccctgtgac aatctcccat tgctttgctg caaccggtat
     1681 ccgaacaact tcacgggtat caatcctgga aaagagatta ttgtcaacct ggtctgcgag
     1741 tgtcgtaacc ctgttaattc caagaaatcc gccttctttt tcaagaatat tctggtcccg
     1801 caatgtttta taggtgtcga tacggtaata ggccgtgtgc aacgcttttt ccttttcagt
     1861 cagttcctgc tgctggccgg caatgatgct gttctgccgg tcaaccgcgc tgtcgagcag
     1921 gttgacctgc gtagcgagct gatcctttgc ctggatcgta taatccagat ctctccgcaa
     1981 cacctctttt tcagcaatga gctggtcaag ctgctgctgg taaaccgtta ccttcgcctg
     2041 gtattctttg atgctcttct ggtagcccga gatgcgggag tctttttcgt cgatctggtc
     2101 attcaggctc gcaatcagct tattattttc atcgatcagg tttttaatga aattgatctc
     2161 attttgaatc tgatcctcag gggccaggtc gctgttgtac tccggtcctg caaatccctg
     2221 gctgatcatg gattctttct cccggatact ggccaggttc ttttctatcc tgttatatac
     2281 cgccagcacc ttctcctcgt ttgccttcat aatcccttct ttttcagcaa gcagctgttt
     2341 cttatcgttt ctctcaactg taaaagcaat gattcccccc gcagcgatga gtaccatcag
     2401 cagaatagcc aggggtaatg agattgtcct gtttttcaat tgattgttca tttttttatc
     2461 ctccagaatt ttagtgagtt ttataatttt tataatagta tgaagatgtg ctctccataa
     2521 gcgtgccaac agagaaaaca gaccataact aattaataac caatatgata ggaaaattcg
     2581 gaaggaagtc tttatcgcat tctgaacaat ttcttctcaa tttgagaaat ccggatgact
     2641 actgatcctt gagcatgcga tagatcgtag ccatgctgat ccccagcttc gaagcgatga
     2701 ccggcatgtt ccgctgatat ttattgagat aatagtcgag tattctctta ttatattgtt
     2761 tcatactcat ttcttccccg atgatttcat atagaatatc ggcctggctg aaaaggatat
     2821 ccgatgcttt gatagaagag cccgacgaca taatgacgga cagttcgatc accgatcgca
     2881 gttccctgac atttccgggc cagggatatt gcatgagccg gtcgaacgcc tcagcagaga
     2941 tctccttgac cggcaggttg ttctctttgc aaaagttttc aatgaaatat tttgaaagca
     3001 acaggatatc ctttcccctt tcgcgcaacg gcgggagttc gatggggagc ccgaaaatgc
     3061 ggtaaaacag gtcctggcgc aaggtccctt tttttacctc ctgctgcaga tttcgattgc
     3121 tggcaacaat gagccggcag tcgaatctga ccggttcatt gcttccaact cggatgattt
     3181 ctctttcctg caaggccctc agcagcttgg gctggaagct gatgtcggta tcggtgatct
     3241 catccaggaa aattgtccct ccctcggctt cctcaaattt tcctttgcga cgggccaacg
     3301 cgccggtgaa agcccctttc tcatggccaa aaaactcgct ttcaaacagt tctgcgggaa
     3361 tggcactcat gttcacagcc acaaatggct tattttttcg ggcagagtta tagtggatcg
     3421 ctttagcgat tacctctttg cccgtacctg tttccccggt gatggtcacg gtaatattgc
     3481 tctccgcccc tttgtgcatt atttccagga ctttagccat ggccgggctg ttcccgatga
     3541 tcgctttctc aaaaccatac ctggtgacca cctctttctc aagaacctgg attctttttc
     3601 tgagcccggc gttatccctg attttctgaa tggtattgtg taaacgagcc tggatatttt
     3661 cactctttac caggtaatcg aaagcgccct ctttcaatat gtccacagcg gtctgcacat
     3721 cttcctgccc cgagatcacg ataatttcta tcgattcatc gtgaagcttg atctttccaa
     3781 gtaaatcatt tccttttata tccggaagcc ggtaatccag cgtgattaca tccggggttt
     3841 cgtgcaggct tttcagcaga tcggttccgc tggaaaaacc ttttacctca tattcagggt
     3901 tcatgctcag gtggtgtacc aggaaccggt tgtaccattc atcatcctcc acgacaaaaa
     3961 tcctgaaatg ttctttctgc atatcttatg gggtcttaaa ttccggttct gaaaggatgc
     4021 ttttttgtat ccgttcaaat tcctcctttg ctttgcttac aatttctttt aaaaacttct
     4081 tttttccttc gacagaatgt tcttcggcct ctttcaaaag gctgtatagc ttgtcggccc
     4141 tgaggtgacg acatgaggcg ctgatccggt gcgctacggc tgcagcatca tcccatgatc
     4201 cgttatcgat tttttcctcc atctctttaa ggcctgtttg tgtgttttcc aggaataaat
     4261 ataccatctc tttaaaaaat gcgacattgc cctcgcagct ctttctaagt gcctccaggt
     4321 caaccctgca aacctccctg cttgggcgtg tcaggtcgag aatggccgat atcagccgct
     4381 gctcttcaaa tggcttggcc atccagtgag tgatccctgc tggttttaat cgttcccggt
     4441 cttcttcggt aatagcggcg ctcagtgcaa tgatgggcac cgtcttcccc ctgccggcaa
     4501 agatacggtt catttcaatg gcggcatccg gaccactttt ccccggcatg cgcatgtcga
     4561 ggataaccag gtcgggatca ttgtcgtgcg ctgcctgtat ggcttcctgc ccgttgctgc
     4621 actcgatgac cctgcaatga tatttgtcaa ggatcatgag gagaaggctc cggttatagg
     4681 tgtcgtcatc cgctacaagg atattcatac ccttcagccg ggccggatca ggcaaatagc
     4741 cggaaggggc cggttcgctg ctcccggtgg caagcccgaa tgggatttcc accctgaagg
     4801 tcgatccttt tccgggtgtg ctggtcactt cgagctttcc atccatgagt tcgaccagtt
     4861 tggaggtgat attgagcccc agtccggaac cttccgattt tcctagagcc acagctcccc
     4921 gggtgaattc cgaaaagatc ctctccattt catcctgtgc gattccggca ccggtgtctg
     4981 tcacctcaaa gacagcacgg aaggtatcgg cgtctgccgg ccccgacctg gcttttatgg
     5041 tcactgagcc ctggtcggta tacttgatgg cattgctgat caggttgaac aggatttgcc
     5101 ggagcctgac cggatcgccg acgagtttca aagatacttt cgggtcgatt tcggccatga
     5161 atttcaggtt cttttcttcg gctgcacgcg aaaaccaatg gtgcatctcg cgtgtgattt
     5221 ccgcaggatt gaattccgta tggatcagtt ccattttccc ggcctggatg cgggaaaggt
     5281 cgagcaggtt attcagaagc tccagcaggt aatccgacga ttttctgaca atcccgagct
     5341 gggcattcag ctccggttcc aggggcattt tcaggatctg gtccaggtat ccggagataa
     5401 tattcatagg cgtccggatt tcatggctca tattggccag gaactgctgg cgggcctgcg
     5461 ccagcgcttc agcatcctcc ctcgcctttt tcaggatttt ccggtattca ttatttctac
     5521 ggatgtataa aaagatcacg gtcccggcta aaatcagcaa aatagctgat gcaatcccga
     5581 acaccaggat gatgaaccgg acctttgagg cttctttttc aaccctgtcg gtatgttctt
     5641 tcacatattt tgccgcctgt tggtccaggc cggagatcat agctctcagc ttatcggtga
     5701 tcacccggtc gtaggttgtc c
//
LOCUS       DAOPNH010000228         5693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16363_length_5693_cov_7.795495, whole genome shotgun sequence.
ACCESSION   DAOPNH010000228 DAOPNH010000000
VERSION     DAOPNH010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16363_length_5693_cov_7.795495"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKZ74_11235"
     CDS             complement(<1..295)
                     /locus_tag="PKZ74_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93369.1"
                     /translation="MPTLSKSTFLRGSQCRKSLYLHFNRPELKDPISPMQQAIFSQGH
                     EVGKLAQQLFPGGTDAGLYVPDRYTQSIALTRRLIEEGTEVIYEAGFAVDDLHC"
     gene            complement(479..760)
                     /locus_tag="PKZ74_11240"
     CDS             complement(479..760)
                     /locus_tag="PKZ74_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPM93370.1"
                     /translation="MFKVNEYFDGKVKSIAFTTPDGPATLGVMAPGEYEFGTSTVEYM
                     TVVSGMMTVKLPGEAEFRDYKPFDTFIVAKDSKFQLRIVTDAAYICLYK"
     gene            complement(852..1859)
                     /locus_tag="PKZ74_11245"
     CDS             complement(852..1859)
                     /locus_tag="PKZ74_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM93371.1"
                     /translation="MILVTGATGLVGSHLVYELVRSGQSVRALKRPGSDITMIRKVFE
                     LHQEDLFSKIEWVEGDILDIYSLEDAMAGVDTVYHCAALVSFLPKDRKKLMRINTEGT
                     ANVVNAALEKKIATLCHVSSIAALGRPEDQNDVIDESLVWKTSKHNSAYAVSKYGAER
                     EVWRGTAEGLDAVIVNPSIILGVAGPGMGSSRLFNTVWNGLKFYPPGKNGFVDVRDVV
                     RAMILLTDSHIRNERFILNAVNLDYKTLFTLMARELERPAPSISVSPFFSGFAWRLEK
                     IRSAITGIKPLITRETANTAVQQFEYSNLKIRKAIDFEFIPIEETLRHYCHIFKSTLN
                     S"
     gene            1907..3184
                     /gene="tyrS"
                     /locus_tag="PKZ74_11250"
     CDS             1907..3184
                     /gene="tyrS"
                     /locus_tag="PKZ74_11250"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPM93372.1"
                     /translation="MNFIEELRWRGMIHDMTPETEELFKKDEMVVGYVGIDPTADSLH
                     IGHLVSIMMLKHLQLAGHKPLALVGGATGMIGDPSGKSEERNLLSEEIIRHNEHCIKR
                     QLEKLLEFGNKPNAAEMVNNYDWMKEFTFLGFIRDVGKHITVNYMMAKDSVKKRLETG
                     ISFTEFTYQLVQGYDFLYLNEHKNCKLQMGGSDQWGNITTGTELIRRKNGGDAYALTC
                     PLITKADGGKFGKTETGNVWLDPVRTSPYKFYQFWLNTSDEDAEKYIRIFTLFTREEI
                     DEMIARHKEAPHQRELQKALAKDLTVRVHSEADYQAAIDASAILFGAGTTETLKGLSE
                     DLFLSVFEGVPQSEVSKVKLEQGIPLLEFLADETGIFPSRGEARRTVKENALSINKMK
                     VVEDKMVGVGDLLNEKYILVQKGKKNYFLVVAV"
     gene            3685..>5693
                     /locus_tag="PKZ74_11255"
     CDS             3685..>5693
                     /locus_tag="PKZ74_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM93373.1"
                     /translation="MKNFHLFRFIANSLKNWICTGLGICLVATFNVTSAQTVYQAGKY
                     SLLLENGMYYDISGPDTTKVTTNHMAIKLREGISENERSEFLSRHPLSYSGMGTTTSV
                     IIFNIDSLANFVNLIENIANDPDVELYDINHLVPICDTGKPNDGHDQVDYLLLNSYPN
                     LLWPYINTNLFEAWNFENGDSSIIVAEIDNGIWIDHPDISYGFDNYSNLWNNHDEING
                     ISNYDDDGNGFEDDFHGWNFTEGNGDLMHDNIFADKGTHGTIMSGIIAAKTNNELGIW
                     GTAGGYSTPGIRILPVKCNNLEDPNLLETDYIIAGIDYAIALGARVINMPFAGADFPG
                     IHNKISEYFNQGNVVFLAATGNDWQDDIDGEVYYPAKYEEVIGVGATECNPQDLNSLE
                     TRWILGHSWSSQYGVGIDICAPGSPFTTDFIWDGNQYSPTYNYYTIEDNGSTSRAVSF
                     ASGVVALILSANPCLTNEEVMDTLRNTAYKFPYPGLEWEYGWNKYYGYGRIDALKALQ
                     PVIIPPIEENTTWNSDMKIYNEINIENGAELTIQNCTISMSSWAKIIVKPGGTLILNN
                     CTLTGICNSIMWPGIEVWGYPNAPQFKYPLLPYPQGVIILNGATIENAVTAINLWKPD
                     DYTMTGGIVEAYNSTFKNNVMSAHALYYRNFNPLYPTQELDYYGI"
BASE COUNT         1576 a         1271 c         1322 g         1524 t
ORIGIN      
        1 agcaatgcag gtcatcaaca gcaaaaccgg cttcatagat aacctccgtg ccctcttcga
       61 tcagtctgcg cgtcaatgca atgctttggg tgtacctgtc gggaacataa aggccggcat
      121 cggtccctcc ggggaaaagc tgctgtgcca gtttacccac ctcgtgaccc tggctgaaga
      181 tggcctgctg catcggggaa atgggatcct tcagctcagg ccggttaaag tggagataca
      241 gcgatttacg acactgcgat ccgcggagaa aggtcgattt ggaaagagtt ggcattgcgg
      301 tggttgattt ggaggatttc tcaaaagtct tttatttaac cacaaagtcg cacaaagtac
      361 gtcacaaagt cgcacaaagt atctgattat ctaataataa gcttcgtgcg ccttcgtgaa
      421 atccttggtg ttcctttgtg gtttattact tttgatataa cctcagcaag aactctcatt
      481 atttatataa gcaaatatat gctgcatcgg taacgatccg gagctggaat ttcgagtcct
      541 ttgccacgat gaatgtgtca aagggtttgt aatccctgaa ttccgcttcg cccggcagct
      601 tgacggtcat catgccggac accacggtca tatattctac cgtggaggtt ccgaattcat
      661 actcgccagg cgccataacg cctaaagttg caggtccgtc cggggtggta aaagcaatcg
      721 atttcacttt tccgtcgaaa tattcattga ctttgaacat gatattttga tttttggtaa
      781 aaatacaaat ttatccctac agactgcacc accgggctgt attgcccgaa tcgaccggtg
      841 cgaaatccaa atcaggaatt cagagttgat ttgaagatat ggcagtaatg ccggagggtc
      901 tcttcgatgg ggatgaattc aaagtcaatc gccttcctga ttttgaggtt cgaatattca
      961 aactgctgaa cggctgtgtt ggctgtttcg cgggtgatga gcggttttat gccggttatg
     1021 gccgatcgga tcttctcgag cctccaggca aaaccggaaa aaaatggact gaccgatatt
     1081 gagggagccg gcctttccaa ttccctggcc atcagtgtaa acagcgtttt gtaatcaaga
     1141 tttaccgcat tcaggataaa ccgttcattg cggatatggc tgtcggtcag aaggatcatc
     1201 gcccgaacca catcgcgaac atcgacaaaa ccgttttttc cgggaggata aaacttcagg
     1261 ccgttccaga cagtgttgaa cagcctcgaa ctgcccattc ccggaccagc cacgccgaga
     1321 ataatagatg gattgacgat caccgcatcg agcccttcgg cggtgccgcg ccagacctct
     1381 cgttcggcgc cgtatttgct gacggcataa gccgaattat gttttgaggt tttccatacc
     1441 aggctttcgt cgatcacatc gttttgatct tccggccggc ccagtgcggc tatggaggaa
     1501 acatggcaca gcgtcgcgat cttcttctcc agcgccgcgt tcaccacatt ggctgtccct
     1561 tcggtattga tccgcatcag ctttttccgg tccttcggaa gaaacgaaac cagggcggcg
     1621 cagtggtaaa cggtgtcgac ccctgccatc gcatcctcca gcgaataaat atcgaggata
     1681 tcgccttcaa cccattcaat cttactaaaa aggtcttcct ggtgaagttc aaataccttt
     1741 ctgatcatgg taatatcgct gcccggcctt ttcaatgccc tgaccgactg ccccgaccgg
     1801 accagctcat ataccaggtg ggatcccacc aggcccgttg cgcctgttac cagtatcatg
     1861 cggcaaaatt aacgcttttt cctacctttg cataaacttt aggcttatga actttatcga
     1921 agaactccgc tggcgcggga tgatccacga catgacgccc gaaacggaag aactgtttaa
     1981 aaaggacgaa atggtggttg gttatgtggg tatcgatccg acggccgatt cgcttcatat
     2041 cggccacctg gtgagcatca tgatgctgaa gcacctgcag cttgccggcc ataagccgct
     2101 ggcgctggtc ggaggggcaa cgggcatgat cggcgacccg tcggggaagt cggaagaaag
     2161 gaatctgctt agcgaagaga tcatccgtca taacgaacat tgtatcaaaa gacagcttga
     2221 aaagcttctc gagttcggta acaagccgaa tgccgccgaa atggtcaaca actacgactg
     2281 gatgaaagag ttcaccttcc tgggttttat ccgcgatgtc ggcaagcata ttacagtcaa
     2341 ttatatgatg gcgaaggatt cggtaaagaa gaggctcgaa acagggatct cgttcaccga
     2401 attcacctac cagctcgtac agggatatga ttttctctac ctcaacgagc ataagaactg
     2461 caaactgcag atgggcggct ccgaccagtg gggcaacatc accacgggca ccgagctgat
     2521 ccggcgcaag aacggcggcg acgcctatgc cctcacctgc ccgctgatca ccaaagccga
     2581 cggcggcaag ttcgggaaga ccgaaaccgg caacgtatgg ctcgatccgg tacgcacctc
     2641 cccatacaaa ttctaccagt tctggctgaa tacttcggat gaggatgcag agaagtatat
     2701 ccggatcttt acgttgttta cgcgtgaaga gatcgatgaa atgatcgcac gccacaaaga
     2761 ggcgccgcac cagcgcgagt tgcagaaagc gctggcaaag gaccttacgg tgagggtgca
     2821 ttccgaagcc gattaccagg ctgcaatcga tgcgtcggcg atcctgtttg gcgccggcac
     2881 gaccgaaaca ttgaaaggcc tctccgagga tcttttcctc tccgtttttg aaggggttcc
     2941 gcaaagtgaa gtatctaaag ttaaactcga acaaggcatc ccgctcctgg agttcctggc
     3001 ggatgagacc ggaattttcc cgtcccgggg cgaggcgcgg cggacggtaa aagagaatgc
     3061 gttatcgatc aataaaatga aggttgtgga agataaaatg gttggcgtgg gtgatctttt
     3121 gaatgaaaag tatatcctgg ttcagaaagg gaagaagaat tattttctcg ttgtagctgt
     3181 ttgattttta accacataga cacaacttgt cccgaaacct cgggataggg cacaataggt
     3241 tacacataga tctgttgtgc tttctattgt gttcaaaggg tcagtgtgtt gaatggcgac
     3301 gtctcaaacg gtgtgccctg gtaaaggaag agataaggat tgagactcca tctgcccgaa
     3361 atgagatgcc atctcgattt tgagatggag tctccttgca atcgagatgc catctcatcc
     3421 aaaccagatg gcgtctcaaa ctaaatagtt atcaaccgca cttgactttc cattcaaatc
     3481 agcttatctt tatgccgatt agtccacttc cgcatttttc tatcaacaaa attaatcgcc
     3541 atgtctgctt ttggcacttc cgtagagaga gagagagaga gagagagttt aaattctcac
     3601 attcagcagg ttttttgtta ttctgtcaac tttcgaatat cctatttatt cctttctgtt
     3661 tattaaccgt taatccgaat aattatgaaa aattttcatt tgtttcgatt tattgccaat
     3721 agcttaaaaa attggatctg caccggactt ggaatttgtc ttgttgcgac attcaatgtg
     3781 actagtgcac aaaccgtgta tcaggcaggt aaatattctt tacttttaga gaatggaatg
     3841 tattatgaca tttccggtcc cgatacaact aaagtgacga caaaccatat ggcgatcaaa
     3901 ttacgtgagg gcatttcaga aaatgaacgt tcggaattcc tttcacgtca tcctctttcc
     3961 tattcaggaa tgggtacgac taccagtgta ataatattca acatcgatag cctggcaaat
     4021 tttgtcaatc ttattgaaaa tattgcgaat gatccggatg tggaattata tgatattaat
     4081 cacctggtcc caatttgcga taccggaaaa ccaaatgatg gtcatgatca ggttgattat
     4141 ttgctgctga acagctatcc taatttactt tggccatata taaatacaaa tctctttgaa
     4201 gcctggaatt tcgaaaatgg cgactccagt ataattgtag ctgaaattga taatggcatc
     4261 tggattgatc atccagatat ttcatatgga ttcgataatt attcaaacct atggaacaat
     4321 catgatgaaa tcaacgggat atcgaattat gacgatgatg gcaatggatt cgaggatgac
     4381 tttcatggat ggaattttac agaaggcaat ggtgacttga tgcatgataa catttttgct
     4441 gataaaggga cgcatggtac gataatgtca ggaataattg cagccaagac aaataatgaa
     4501 ctgggaatat ggggcactgc cggcgggtat tcgactcctg gtattaggat tttaccggtt
     4561 aaatgcaaca atcttgaaga cccaaatctt ctcgaaaccg attatattat tgctggcatt
     4621 gattatgcaa ttgccctggg agcaagagta attaatatgc catttgccgg tgctgatttt
     4681 cctggtattc acaacaaaat atctgagtac tttaatcaag gaaatgttgt tttccttgct
     4741 gcaaccggaa atgactggca agatgatatt gatggagaag tatattatcc ggcaaagtat
     4801 gaagaagtaa ttggggtcgg cgctactgag tgcaaccctc aagacctgaa tagtcttgaa
     4861 acacggtgga ttcttggaca ttcctggtcc tcacaatatg gagttggaat tgatatttgt
     4921 gcaccgggat caccctttac aactgatttc atatgggatg gaaaccaata ttcacctact
     4981 tataattatt ataccattga agataatggt tccacttcaa gagcagtatc atttgcttca
     5041 ggtgtggtcg cattaattct ttccgcaaat ccgtgtctca ccaacgaaga agttatggat
     5101 acattaagaa acactgctta taagtttcct tatccgggtc ttgagtggga atatggctgg
     5161 aacaaatatt atggatatgg aaggatagac gcactaaaag ctctgcagcc cgttatcatt
     5221 cctcccatcg aagaaaacac aacctggaac agcgacatga agatttataa tgaaattaat
     5281 atcgaaaatg gagcagaact gaccattcaa aattgcacca tttcaatgag tagttgggca
     5341 aagatcattg ttaaaccagg agggaccttg attctaaata attgcacttt aaccggtata
     5401 tgcaatagca ttatgtggcc aggcatcgaa gtctggggtt atccgaatgc tccgcaattc
     5461 aagtacccgc ttttacccta tccacaaggt gtaatcattc tcaatggtgc aacaattgaa
     5521 aatgccgtta cagccataaa tctatggaaa ccggatgatt acaccatgac gggaggcatt
     5581 gtggaagctt acaattctac ttttaaaaac aatgtcatgt ctgcgcatgc actgtattac
     5641 cgcaacttca atccattata tcccacacag gaacttgatt attacggaat att
//
LOCUS       DAOPNH010000229         5689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16391_length_5689_cov_6.122826, whole genome shotgun sequence.
ACCESSION   DAOPNH010000229 DAOPNH010000000
VERSION     DAOPNH010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16391_length_5689_cov_6.122826"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKZ74_11260"
     CDS             complement(<1..182)
                     /locus_tag="PKZ74_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93374.1"
                     /translation="MKKLLLLSLSLLFTWAAFSQGALYTDDFEGYAVGDYIAESNPTW
                     WTTWSNDPGSGEDGYIS"
     gene            complement(292..1245)
                     /locus_tag="PKZ74_11265"
     CDS             complement(292..1245)
                     /locus_tag="PKZ74_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPM93375.1"
                     /translation="MQNKLYPWNHLRRFNSYAEHLKKQFGGRVQKLAIDAGFTCPNRD
                     GSKGIGGCSYCDNDAFNPSYCKPEKTVSQQIEEGIEFHRKRYRRAAKYLAYFQPYSNT
                     YAPLPRLREIYRQAIEIPEIAGIVIGTRPDCIDADKLEYLAELAKTHYVMLEFGIESC
                     YDETLRRINRGHTFPETVEALDMAKHYGINTGIHLIFGLPGETREMMLHQAEIISRLP
                     VKTIKFHQLQIIRGTRMEREYREKPDDFTLFTLEEYIDFVIDFTEKLNPSIVIERFAG
                     EVPPRFLAGPGWGLIRNDQILRMIESRMEERETWQGKRNRE"
     gene            complement(1235..2773)
                     /locus_tag="PKZ74_11270"
     CDS             complement(1235..2773)
                     /locus_tag="PKZ74_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPM93376.1"
                     /translation="MMKQHPKGLLAAALANMGERFGFYTMMAILVLFLQAKFGLSGTH
                     AGIIYSVFYFSIYILAFVGGLIADKTRNYKGTIMIGQFVMAAGYLLIAIPTKTPVPNQ
                     VLFLIISCIGLFTIAFGNGLFKGNLQALVGQMYDNPTYGKMRDSGFSLFYMFINVGAL
                     FAPIAAIAMRNGWLMRHGYEYNPNLPELCHQYLKGTITPEAATKLQTLASEVSTAGPV
                     SDLTLFANDYLNVFATGFHYAFGISIIAMLISLTIYLVNKKIFPDPSLKVKTVVNGEH
                     HEITMDPKEVKQRLYALFAVFGIVIFFWFSFHQNGLTLTYFAKDYTDLSGIRINLGVM
                     EIKGAEIFQSINPIFIVLLTPIIMGIFGWLRARGKEPSTPKKIAIGMLIAATAYLVMT
                     IGSMGLPLKSEVEAAGGMADAERVTPMLLIGTYFILTVAELFISPLGISFVSKVAPPQ
                     YQGVMQGGWLGATAIGNQLLFIGAILYESVPIWLTWSVFVVACFLSFLTMLFMVRWLE
                     RVAK"
     gene            2943..3458
                     /locus_tag="PKZ74_11275"
     CDS             2943..3458
                     /locus_tag="PKZ74_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM93377.1"
                     /translation="MSPQEFKIEVIPLKNKLYRLSLRMLGATEEAEDAVQDAFVKLWT
                     KRNELSGYSSVEAFAMTVTKNLCLDRIKSKSFQTERISDKLNSSLGNDPGNVVENSDF
                     NGFIHRIIARLPDHQKVIMHMRDVEDYEYEQIAEVMGLNVNAVRVNLSRARKKVRDTI
                     LKMHDYELARN"
     gene            3439..4002
                     /locus_tag="PKZ74_11280"
     CDS             3439..4002
                     /locus_tag="PKZ74_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93378.1"
                     /translation="MNLQEIEKLLMKYFEGETSLPEEKILRDFFSGGDVPEKWMNLKV
                     YFSIIREEKKVLLENPELENRLETIPEAEKIAPLIDLRRPWIYWMTGVAASILILIAI
                     FVRFDPFPKKIEDTYKDPQVAYTEARKILQFVSSKFNQGTRSLKSVNAIETGLTQLKP
                     VGSYDKAVSEVNRLSDVEKVEKLIINN"
     gene            4053..4592
                     /locus_tag="PKZ74_11285"
     CDS             4053..4592
                     /locus_tag="PKZ74_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPM93379.1"
                     /translation="MKRTILAALTMLLLMSAQMIYAQNSSALALFDKYGGKEGYTTVS
                     ISKELFGMFADIESDDPDMKEVKTMMEQLEGIKILMFETENADDAELARFKSEVGKLQ
                     VTGYSELMVVKQTGEEVKFLAQKKGDKIGELLLVINGEKEAGFITISGLIDMSTIGKL
                     SKTMNFEGMDNLEKLNDNK"
     gene            4615..5157
                     /locus_tag="PKZ74_11290"
     CDS             4615..5157
                     /locus_tag="PKZ74_11290"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPM93380.1"
                     /translation="MKKLTLIILVAVLPVFAMAQKTPFTQLYDKYVSEPGIQTTQILP
                     GSTSFEWEKTADNTAIREMLNSIESIRIMKIQSDSTKVSPEKIWKNMQKAASEDAYRE
                     ILTISADKVQMRMLMLRGEEGRTREVALIAREGDGVFMMTMTGNMDFSSMFSPENMKN
                     FREIGEYYLENHSGCNKAEE"
     gene            5386..>5689
                     /locus_tag="PKZ74_11295"
     CDS             5386..>5689
                     /locus_tag="PKZ74_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPM93381.1"
                     /translation="MDIPSKYNPKEIEEKWYRHWMDNKYFSSKPDEREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDILVRRARMQGKNAEWLPGTDHASIATEAKVVASLRAKGID"
BASE COUNT         1664 a         1292 c         1360 g         1373 t
ORIGIN      
        1 gaaatgtatc cgtcttcgcc cgaaccggga tcattgctcc aggtggtcca ccaggtcgga
       61 ttactttcag cgatgtaatc tcccaccgca tagccttcaa aatcatcggt gtacaaagca
      121 ccttgtgaga aggcagccca ggtgaagagc aacgataacg atagtagtaa taactttttc
      181 ataaagcttt attttttggt taacactata agaatgcaca aaggcaaata taaaaagata
      241 attccggaaa tgacagaaaa aaaaccacca aacaaattca accctatttt actattccct
      301 attccttttt ccctgccacg tctccctttc ctccatcctt gactcaatca tcctcagtat
      361 ctgatcattc cgtataagcc cccagcccgg accggccagg aatcggggag gtacctcgcc
      421 ggcaaaccgt tcgataacga tggaagggtt taatttttcg gtaaagtcga tcacaaaatc
      481 gatatactct tccagggtga aaagggtaaa gtcgtccggt ttttccctgt attcccgttc
      541 catcctggta ccccggatga tctgtaactg atggaatttt atagtcttaa ccggaaggcg
      601 tgaaatgatt tctgcctgat gtagcatcat ttcgcgtgtt tcgccgggaa gtccgaagat
      661 caggtgaata cccgtattga tgccgtaatg ttttgccata tccagggctt ccaccgtttc
      721 cggaaacgta tgacccctgt tgatccggcg aagtgtctcg tcatagcacg attcgatccc
      781 aaactcaagc atcacataat gtgttttggc taattccgca aggtattcca gcttgtcagc
      841 gtcgatacaa tcgggccggg ttccgatcac aatccctgct atttcaggaa tttcgatggc
      901 ctgccggtag atttcgcgaa gcctgggcag gggagcatag gtattcgaat aaggttgaaa
      961 ataagccagg tactttgcag cccgccggta tctcttcctg tgaaattcaa tgccttcttc
     1021 aatctgctga ctgaccgttt tttcgggctt gcaataagaa gggttgaagg cgtcgttatc
     1081 acaataggaa caaccgccaa tgcctttgga cccatcgcgg ttgggacagg tgaagcccgc
     1141 atcgatggcc agtttctgca cccttcctcc gaactgcttt ttcagatgtt cggcatagga
     1201 attaaaacga cggagatgat tccagggata caacttattt tgcaacccgt tccagccatc
     1261 ttaccatgaa caacatggtc aggaaggaga gaaaacaggc tacgacgaat acgctccagg
     1321 tcaaccagat cggcacgctt tcgtaaagaa tggcgccgat gaacagcaac tggttcccga
     1381 tagcagtcgc cccgagccat cctccctgca taacgccctg gtactgcggc ggagctacct
     1441 ttgacacaaa tgaaatgccc agcgggctga tgaaaagctc tgcgaccgtc aggatgaaat
     1501 aggtgccgat cagcagcatt ggcgtgacgc gctcggcatc cgccattccg ccggctgcct
     1561 cgacctccga tttaagcggt aatcccatgg atccaatagt catgaccaga taagcagtgg
     1621 ctgcgatcag catcccgata gcgattttct tcggagttga tggttctttg ccccgggctc
     1681 ggagccatcc gaaaattccc atgataatag gcgttaacag gacgataaat attgggttga
     1741 tcgactggaa aatttccgct cccttgatct ccatgactcc aagattgatc cggataccac
     1801 tgagatcggt ataatctttg gcgaaatagg tgagtgtcaa tccgttctga tggaaagaga
     1861 accagaagaa gataacaatg ccgaacacag cgaacaaagc atacaatctc tgtttgacct
     1921 ctttgggatc catggttatt tcatgatgtt caccattcac cactgttttg accttgagcg
     1981 acggatcggg gaaaatcttc ttgttgacca ggtaaatcgt cagggagatc agcatggcaa
     2041 tgatcgaaat accaaaagca taatggaatc cggtagcgaa aacgttaaga taatcattgg
     2101 cgaataatgt caggtcgctt accggtcccg cagtgctgac ctcggaggcc agggtctgca
     2161 gtttggttgc tgcctccggc gtgatcgtgc ccttgagata ctggtggcaa agttccggca
     2221 ggttgggatt gtactcatac ccgtggcgca taagccagcc attccgcatg gcgatggcgg
     2281 caataggtgc aaacaaagcc ccgacattga tgaacatgta aaagagtgaa aatccggagt
     2341 ctctcatttt tccgtatgtg ggattgtcgt acatttgacc gaccagcgct tgcaggttgc
     2401 ctttgaacaa tccattaccg aaagcaatag taaacagtcc gatacaactg atgataagaa
     2461 acaagacctg gtttggcacc ggcgtctttg tcgggatggc aatcaacagg taaccggctg
     2521 ccatgacaaa ctggccgatc atgatggtcc ccttgtaatt tcttgtcttg tcagcaatca
     2581 atccgcctac aaaagccaga atgtagattg aaaaatagaa tactgaataa attatccctg
     2641 catgagtgcc tgaaaggccg aattttgcct gtaagaacaa tacgaggatc gccatcatgg
     2701 tgtagaaacc gaacctttct cccatgttgg ccagggctgc agcgagcaac cctttgggat
     2761 gttgtttcat cataacttta gattttagtt tgtttataga tttaatttga atccggaaag
     2821 tttaaggatg acaaaaataa ttaaaaatcg atcttttcaa agttccgttc agttttcggc
     2881 aaaatttgca gaaatttata caggtgatgt aatgaagtct ggtaaaattc gtcttacccg
     2941 tgatgagtcc acaagagttt aaaatagagg tcattccatt gaaaaacaag ttgtacaggc
     3001 tttcgctgag aatgctcgga gcaacggagg aagcagaaga cgccgttcag gatgcctttg
     3061 ttaaactctg gacaaaacga aatgaactta gtgggtattc cagtgttgaa gcctttgcaa
     3121 tgactgtgac aaaaaatttg tgccttgacc ggatcaaatc gaagagtttt caaaccgaaa
     3181 ggatttcgga taaactgaat tcatcccttg gaaatgaccc gggtaacgtg gttgagaatt
     3241 cggatttcaa tggattcatc caccggatca ttgcccggtt gcctgatcat caaaaggtta
     3301 tcatgcacat gagagatgtt gaagattatg aatacgagca gattgccgaa gttatggggc
     3361 tgaatgtcaa tgcggtaagg gtgaacctgt caagggccag aaagaaagtc agggatacga
     3421 tcttaaaaat gcacgattat gaacttgcaa gaaattgaaa aattactgat gaagtatttc
     3481 gaaggagaga cttcccttcc ggaagagaaa attctgagag attttttctc tggcggcgat
     3541 gttccggaaa agtggatgaa tcttaaggtt tacttttcaa taatcaggga agaaaagaag
     3601 gtgcttctgg aaaatccgga actggaaaac cggttggaaa ccattcctga agctgaaaag
     3661 atcgccccgc tgatcgacct tcgccggccc tggatctatt ggatgactgg tgtcgccgcc
     3721 tccatcctga tcctcatcgc catctttgtc cggttcgatc ctttcccgaa aaaaattgaa
     3781 gatacgtata aagatccaca ggtagcatat acggaagcgc ggaagatact tcagttcgta
     3841 tcctctaaat tcaaccaggg cacccgcagc ctgaaatcgg tcaatgctat cgaaacaggc
     3901 ctgacccagc ttaaacctgt cggctcatat gataaagccg tcagcgaagt gaaccgcctc
     3961 agcgacgtcg aaaaagtaga aaaactaatc atcaacaatt gaacaataga acattagaac
     4021 aatttaataa tcaaacaatt aatttaacaa ctatgaaacg tacaattctc gcagctttaa
     4081 caatgctgct tctgatgagc gcacagatga tttatgccca gaatagctcc gctcttgcgc
     4141 tgtttgataa atatggcggt aaagaggggt acaccacggt gagcatttcc aaagagctct
     4201 ttggcatgtt tgccgatatc gagtccgatg atccggatat gaaagaggtg aaaacgatga
     4261 tggaacaact cgaagggatc aagatcctga tgtttgaaac cgaaaatgcg gatgacgccg
     4321 aactggcacg cttcaaaagc gaggtcggca agctccaggt aaccgggtac agcgaactga
     4381 tggtcgtaaa acaaaccggc gaggaagtga aattcctggc ccagaaaaag ggtgacaaga
     4441 tcggcgaact actcctggtt atcaacggtg aaaaagaggc cggattcata accatcagcg
     4501 gattgatcga tatgagcacc atcggtaaac tatccaaaac gatgaatttc gaagggatgg
     4561 ataacctgga aaagctgaat gataacaagt aacagctcaa aaaaagatca ttccatgaaa
     4621 aagttgacct taatcatact ggttgccgtt ttaccggtct ttgccatggc ccagaaaacg
     4681 ccgttcaccc agctttatga caagtatgtc agcgaaccgg gtattcagac gacgcagatc
     4741 ttaccgggat cgaccagctt cgaatgggaa aagacggctg ataataccgc gatcagggag
     4801 atgctgaaca gcattgaaag tatccggatc atgaaaatcc aatccgattc cacgaaggtt
     4861 tcgccggaaa agatatggaa aaacatgcag aaagcggcat ccgaagatgc ctaccgggaa
     4921 atcctcacaa tctcggccga caaggttcag atgcggatgc tcatgctcag gggcgaggag
     4981 ggaagaaccc gggaagttgc cctgattgca agggaaggcg acggtgtctt catgatgacc
     5041 atgacaggca atatggactt ttcatctatg ttcagcccgg aaaatatgaa gaacttccgg
     5101 gaaatcgggg agtactacct ggaaaaccac agcggctgca ataaggcaga agaataggtg
     5161 aaccaataag agggtgtatc aaaagtccgg tatttcgaaa tgattgcgtg acataaggtc
     5221 gtgctccaac gactaaagcc gttggttcca agaagattgc aaagatggaa ccgacggctt
     5281 cagtcgtcgg actaacaata ataaacgaag atctggactt tagagacggc ctcttttttt
     5341 tctacttttg ccgtttattt tcgataccga ccgatacaca ataaaatgga tattcccagc
     5401 aaatacaacc caaaggagat tgaagagaag tggtaccgcc actggatgga taataaatat
     5461 ttcagttcaa aaccggatga acgggagcct tataccatag tcatccctcc tccgaatgtg
     5521 acgggcgtgc tgcacatggg ccatatcctg aacaacacca tccaggatat cctggtcagg
     5581 cgtgcccgga tgcaggggaa aaacgccgaa tggctgcccg gtaccgacca tgcctctatt
     5641 gctaccgagg ccaaggtggt ggccagcctc agggctaaag ggatcgata
//
LOCUS       DAOPNH010000230         5660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16503_length_5660_cov_6.256378, whole genome shotgun sequence.
ACCESSION   DAOPNH010000230 DAOPNH010000000
VERSION     DAOPNH010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16503_length_5660_cov_6.256378"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKZ74_11300"
     CDS             complement(<1..237)
                     /locus_tag="PKZ74_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPM93382.1"
                     /translation="MISFQLDTKQQEIIEKYRDFTKRWIIPNRMKYDELATFPWDIVK
                     AAYDEKIINASMPVEYGGHGHSIFEGAIASEELGY"
     gene            complement(328..1161)
                     /locus_tag="PKZ74_11305"
     CDS             complement(328..1161)
                     /locus_tag="PKZ74_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM93383.1"
                     /translation="MAYNLLKGKKGIIFGALDEKSIAWQVAVKAFEEGAEFTLSNTPT
                     AIRFGTIDDLAKTCNTIVIPADATKVEDIENVFEKSMERFGGKVDFVLHSIGMSMNVR
                     KKRVYNEVDYDYFKTTLDISALSFHKIIQVAYRLDAIKEWGSVVALSYIAAQRTLYAY
                     NDMADAKALLESIARSFGYIYGREKKVRINTISQSPTMTTAGSGVRVMGGLMDFTERN
                     SPLGNATAEECANYCITLFSDLTRKVTMQNLYHDGGFSSMGMSLKAMTMYNKSFNCEH
                     C"
     gene            complement(1184..2836)
                     /gene="recN"
                     /locus_tag="PKZ74_11310"
     CDS             complement(1184..2836)
                     /gene="recN"
                     /locus_tag="PKZ74_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPM93384.1"
                     /translation="MLTHLSISNYTLIDRLDIEFHNGFSAITGETGAGKSILLDALDL
                     VLGKRAESQVLLDPSKKCVVEASFVIGKYNLGPFFLSNSLDYDEECILRREIHPNGKS
                     RAFINDTPVTLLQMKALGDQLVDIHAQHATTSLQDPLFQLSVLDGYANLNEQLHQFRE
                     RFLALRNLRDHKEMLLDEERKARSSMDYRQFLFDELNAAALQSDEQESLEERLKLVSN
                     AEEIKNTLYFIQGILNEDDNSILNQLTHAESQLNKIEGYYPPLKQIAERLRSNLIDIK
                     DINLEISKLDEDVEFSQELMNDLQARLDLIYKLQHKHQVTNNGSLIEIQNTLKQQLES
                     FTSLEEKINETGKQILAMESALDEAASLLTAARRKTFPELEKEILGYLRTMGMPDAQF
                     EIRWNLSPEMTVFGRDNIEFYFNANLGHSLKPLAEIASGGELSRVMLAIKTVLSGKKS
                     LPTIIFDEIDSGLSGDIAGKVGDVMRDAAKRIQIITVTHLPQIAGKARHHYSVFKSTA
                     RDVTFSNIKYLDDQERITELAKLIGGRETTDASMAAAKELIN"
     gene            complement(2848..3744)
                     /locus_tag="PKZ74_11315"
     CDS             complement(2848..3744)
                     /locus_tag="PKZ74_11315"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPM93385.1"
                     /translation="MKRIIIMLFLLASVIQSYSQEFYCNVDVTSKQIQSSDKRIYESM
                     RNAIYEFMNNRQWTNYNYKFNEKLECSILINVVERASTELFRCEMTIASRRPVFNSNY
                     NSPLFNFIDKNVDFEYIENQPLDFNTGTFSSNLTSILAYYAYIMLGLDFDTFQLNGGN
                     PYYEAALSIVNAASTSSYTGWNSAEGNKNRFWLVENLTNPAYTGIRSFYYDYHMKGLD
                     IMYEKPEEGRKAILQSLGYLQQVKQSRPGLFFLQIISDAKRDEFVNVFSEGLPTEKTN
                     AVKILNELDPSNAMTYQKIIQN"
     gene            complement(3785..4984)
                     /gene="coaBC"
                     /locus_tag="PKZ74_11320"
     CDS             complement(3785..4984)
                     /gene="coaBC"
                     /locus_tag="PKZ74_11320"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPM93386.1"
                     /translation="MLKGKKIVIGVTGSIAAFKVPALIRLFKKEGAEIKVMMTKAATD
                     FVTPLTLSTLSENPVIIEPFDPVDGSWNSHVDLGRWADLYLLAPVSANTLAKMAGGIA
                     DNFFLTACLSAKCPVFFAPAMDLDMYKHPATMKNIQILQSYGNQLIEPSVGELASGLC
                     GAGRMEEPENIFGLVKSYFENKTGPLSGKRVLVTAGPTHEAIDPVRYIGNHSSGLMGF
                     SLAEQAAEAGAIVTLISGPTDLKLAGSSIERINVTSAAEMHHECLAASKESDIIIMAA
                     AVADFRPVSTSSQKIKKSGNSFLLELIPTKDILTEISANRKPGQVIAGFALETENEID
                     HATEKLVKKNLDLIILNSMREEGSGFKTPTNKVSILFKSGQIKHYPLKSKKEVACDII
                     EAISSMI"
     gene            complement(4993..5334)
                     /locus_tag="PKZ74_11325"
     CDS             complement(4993..5334)
                     /locus_tag="PKZ74_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPM93387.1"
                     /translation="MDYKKVKTDLEAITRDTRKFSEQTSNIYETAVIISKRSNQIALE
                     IKDELGKKIEEFTTTNDNLEEVFENKEQIEIARFYERLPKPTLIAVNEYLNDQIYYRN
                     PHREVKRKKAN"
     gene            complement(5339..>5660)
                     /locus_tag="PKZ74_11330"
     CDS             complement(5339..>5660)
                     /locus_tag="PKZ74_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93388.1"
                     /translation="IANLYQKMRLYESAITAYNNILKDYPDTQFKEVILFSIFKSHYN
                     FAQNSIASKQGERYQSALDAYNELVFQFPESKYIREAAPMNRNLQNEIKNYQQLQSQI
                     STNN"
BASE COUNT         1432 a         1221 c         1233 g         1774 t
ORIGIN      
        1 gtatccgagc tcttccgatg caatggctcc ttcaaaaatg ctatgtccat gaccgccata
       61 ttcaaccggc atggaggcat ttatgatctt ttcgtcatat gcggctttaa caatatccca
      121 ggggaatgta gccagttcgt cgtatttcat ccggttgggt atgatccaac gttttgtgaa
      181 atctctgtac ttctcgatga tttcctgctg cttggtatct aactgaaaac taatcatagt
      241 attaagggat ttggttgaga tcaggtattt aattccttaa caaatataaa atattatttg
      301 tatgacgctg gtttaagctg aaaaatctta acaatgctcg cagttaaagc ttttgttgta
      361 catagtcatc gccttcagac tcatacccat tgatgaaaaa ccaccgtcat ggtacaggtt
      421 ctgcatagtc accttgcggg tcaggtcaga aaacaaggtg atgcaataat tggcgcactc
      481 ttccgcagtg gcattgccca gcggtgagtt tctttctgta aaatccatca gcccgcccat
      541 tacccttacc ccgcttccgg ctgtggtcat agttggagat tgtgaaattg tattgatccg
      601 aaccttcttt tcccggccgt aaatgtagcc gaaacttcgg gcaatagact ccagcagggc
      661 tttggcatct gccatgtcgt tataagcata gagtgtcctt tgtgcggcaa tatacgataa
      721 tgcgactacg gagccccatt ccttgattgc atctaaccgg taggccacct gaataatttt
      781 atgaaacgac aaagcggaaa tatccaaagt agtcttgaaa taatcatagt ccacttcgtt
      841 atacaccctc tttttcctga cgttcatcga catacctata gaatgtaaga cgaagtcaac
      901 ctttccgccg aacctttcca tcgatttctc aaaaacattc tcaatatctt ctaccttcgt
      961 tgcatccgct ggaataacaa tcgtattgca ggttttcgcc aggtcgtcaa tggtaccgaa
     1021 acgaatggcc gtgggagtat tggaaagagt aaactcagcg ccttcctcga atgctttaac
     1081 ggccacctgc caggcaatcg atttttcatc gagtgcaccg aaaatgattc ctttctttcc
     1141 tttgagtaag ttatatgcca taatcgttga ttttaaagtt aaactaattg atcaattctt
     1201 ttgctgctgc catcgaggca tcggttgtct ccctgcctcc gatgagtttt gcaagctctg
     1261 ttattctttc ctgatcgtca agatatttga tattggagaa tgtgacatca cgggctgtgc
     1321 ttttaaatac tgaataatgg tgccttgctt tgcctgcaat ttgcggaaga tgcgttacgg
     1381 taatgatctg tattcgtttt gccgcatccc tcatgacatc tcccactttg ccggcaatat
     1441 cacccgacaa gccgctgtca atttcatcga aaattatggt cggcaacgat ttttttccgg
     1501 ataagactgt ctttattgcc agcattaccc tcgaaagttc tcctcccgac gctatctcgg
     1561 ccaggggctt caatgaatgg cccaggttgg cattgaaata gaattcaata ttatcgcggc
     1621 cgaagactgt catctcaggc gacagattcc accggatttc aaattgtgca tccggcatgc
     1681 ccatggtccg caaatacccc agaatctctt tttccagttc aggaaaggtc ttccggcggg
     1741 cagcggtcaa taaagatgct gcttcatcaa gggccgattc catagccagg atctgttttc
     1801 cggtttcatt tatcttttcc tccagagagg tgaatgactc cagttgttgc ttcagggtat
     1861 tctgaatttc gatcaaagac ccgttatttg tgacctggtg tttgtgttgt aatttataaa
     1921 tcagatcaag ccgggcttgt aagtcattca ttaattcctg gctgaattca acatcttcat
     1981 ctaatttact gatctctaaa ttaatatcct taatgtcgat cagattggac ctgagccgtt
     2041 ctgctatttg tttcagcggt ggataatatc cttcgatttt attaagttgg gattcggcat
     2101 gggtcagctg gttaaggatg ctgttatcat cctcattaag aattccttgt atgaagtata
     2161 gcgtattttt tatctcttca gcattggaga ccagtttcag tctttcttcc agcgattcct
     2221 gttcatcgga ctgcagagca gcagcattca gttcatcgaa taaaaactgc ctgtaatcca
     2281 ttgatgacct ggcctttcgc tcctcatcca gcagcatttc tttatgatcc ctgagatttc
     2341 gcagtgccag gaatcgttcc cgaaattgat gaagttgctc attcaggttt gcataaccat
     2401 ccagtacaga caattgaaac aaaggatcct gcaaggatgt ggttgcatgc tgcgcatgga
     2461 tatccaccag ttgatcgccc agtgctttca tttgaagcag ggttacggga gtatcgttga
     2521 taaaggccct cgattttccg ttcggatgaa tttcacggcg aagaatacat tcttcatcat
     2581 aatccagact attggaaaga aagaaagggc ccagattgta ttttccaata acgaaagatg
     2641 cctccacgac acattttttg gagggatcca aaaggacctg ggattcggcc cttttgccca
     2701 gaaccaggtc aagtgcgtcg agcaggatcg atttgccggc acctgtctca cctgtaattg
     2761 ctgaaaaacc attatggaac tcaatatcaa gccggtcaat taaggtgtaa tttgatatcg
     2821 agagatgagt cagcatttac gtggctttta attctggata atcttctggt acgtcatggc
     2881 attggaagga tcaagttcat tgaggatctt cactgcattt gttttttcag ttggtaatcc
     2941 ttccgaaaaa acattgacaa actcatccct ttttgcatcg gagataattt gcaggaagaa
     3001 caaaccgggg cgtgattgtt tcacctgctg aaggtagcca agcgattgca gtattgcttt
     3061 tctcccctct tccggttttt catacattat gtccaatccc ttcatatgat aatcgtaata
     3121 aaaacttctg atacctgtat atgcagggtt ggtcaggttt tccaccagcc agaacctgtt
     3181 tttattgcct tccgctgaat tccagccggt ataacttgaa gtggatgccg cattgacgat
     3241 gctcagagcc gcttcataat agggatttcc gccatttaac tgaaacgtgt caaaatcaag
     3301 cccgagcatg atataggcat agtaggcaag tatcgatgtg agattggatg aaaatgtccc
     3361 ggtgttaaaa tccaggggtt ggttttcaat atattcaaaa tcaacgtttt tgtcaataaa
     3421 gttgaacaat ggggaattgt agttggagtt aaaaacgggt cgcctggagg cgatggtcat
     3481 ttcgcaacgg aacaattcgg tcgaagctct ttcaacaaca ttgatcagaa tggaacactc
     3541 cagtttctca ttgaatttat aattataatt ggtccattgg cggttgttca tgaattcgta
     3601 aatagcattc cgcatggatt cataaattct tttatcggaa ctctgtattt gtttgctggt
     3661 gacatcgaca ttgcaataga actcttgtga gtacgactgt atgacagaag caagcaaaaa
     3721 gagcatgatt atgattcttt tcataaagtc tttatttaat tgacttagtg taaaaataac
     3781 aaaatcatat cattgaagaa atagcttcaa tgatatcaca ggcaacttct tttttacttt
     3841 ttaatggata atgttttatc tgaccggatt tgaagagaat ggagactttg ttggtggggg
     3901 ttttaaagcc gctcccctct tcgcgcatag aattcagtat gatcaggtcc agattctttt
     3961 tcaccagttt ttcggttgca tggtcaattt cattttccgt ttccagtgca aagccggcaa
     4021 taacctgccc cggttttctg tttgcagata tttcggtcag gatatctttt gtcgggataa
     4081 gttctaacag gaatgaattg cccgactttt taattttttg ggatgaggtg ctgacaggtc
     4141 tgaaatctgc cacagcggca gccatgatga tgatgtcgga ttctttgctg gcggcgaggc
     4201 attcgtggtg catctcggcg gcgctggtca cgttgatcct ttcgatggat gaaccggcca
     4261 gtttcaggtc tgtcggaccg gagatgagtg taacgatagc gccggcttcg gctgcctgtt
     4321 ctgccagtga gaatcccatc agcccggagg agtgatttcc gatataccgc accggatcga
     4381 tagcttcatg agtgggtccg gctgtaacca ggactctctt ccctgataat ggtccggttt
     4441 tattttcaaa ataggatttg acaagaccga aaatattttc cggttcttcc attcggccgg
     4501 cgccgcataa tccgctggcc agctctccga ccgacggttc gataagttga ttgccgtagg
     4561 attgaaggat ctggatattt ttcatggtcg ccggatgttt atacatatcc aggtccatag
     4621 ccggggcaaa gaaaacaggg catttcgccg aaaggcaggc tgtaagaaaa aagttatcgg
     4681 caattccacc ggccattttt gccagtgtat tggctgatac cggggccagc aggtagagat
     4741 cagcccatcg ccctaggtcc acatggctgt tccaggatcc gtctactgga tcgaaaggtt
     4801 ctatgataac cggattttca gataatgtgg aaagagtaag cggggtgaca aaatctgtcg
     4861 ctgctttcgt catcattacc ttgatttctg ccccttcctt tttaaaaagc cggatgaggg
     4921 caggaacctt gaatgcggca atacttccgg tgacgccgat gacgatcttt ttccctttca
     4981 gcatcagtca gactaattgg ctttcttccg tttgacttcc ctgtgaggat ttctgtaata
     5041 tatctgatcg ttcagatatt cgttcacggc aatcagggtt ggtttgggaa gccgctcata
     5101 aaaacgggca atctcaattt gttccttatt ttcaaatact tcctcaagat tgtcgttggt
     5161 ggtcgtaaac tcttctatct tcttaccgag ctcatctttg atctcgaggg cgatctggtt
     5221 tgatcttttt gaaatgatca ccgccgtttc gtaaatattg gatgtctgct cgctgaactt
     5281 ccgggtatcc cgggttatgg cttcaagatc ggttttaact ttcttataat ccattaaatt
     5341 aattatttgt tgagatttgt gattgtaatt gctgatagtt ttttatctcg ttttgcaaat
     5401 tcctgttcat cggcgccgct tccctgatat actttgattc agggaactgg aaaaccaatt
     5461 cattatatgc atccaaagca gattggtatc tttctccctg ttttgaagcg atgctgttct
     5521 gtgcaaaatt ataatgagat ttgaaaatgg agaacaaaat gacctcctta aattgtgtgt
     5581 ccggataatc tttaaggatg ttgttatagg ctgtaatggc tgattcatac agtcgcatct
     5641 tctggtacaa attggcaata
//
LOCUS       DAOPNH010000231         5614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16712_length_5614_cov_6.317503, whole genome shotgun sequence.
ACCESSION   DAOPNH010000231 DAOPNH010000000
VERSION     DAOPNH010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16712_length_5614_cov_6.317503"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKZ74_11335"
     CDS             <1..392
                     /locus_tag="PKZ74_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93389.1"
                     /translation="WLREQLASIGAETPIIISLHIPLLTTEAQILRGATAANDRNEVV
                     VNSKEVIDLFNGHNLKLVLQGHLHYYESLHVFGTNYVTAGAVSAAWWEGPFLGTEEGF
                     LLLKAKGDEISWEYVDYGWQAGKSSPL"
     gene            complement(337..1095)
                     /gene="rhaD"
                     /locus_tag="PKZ74_11340"
     CDS             complement(337..1095)
                     /gene="rhaD"
                     /locus_tag="PKZ74_11340"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF002963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HPM93390.1"
                     /translation="MQLNKTNQGAQGVAAGIAEVASLLWQKGWAEASGGNISVNVTEF
                     YPNVRIDFRTYPMIPLPVKYPAIAHNYIFITSKGSRMRTLAKDPGSGICLVKISKSGE
                     AYQVLFEDPENPLVPSSELPSHFAIHNLLVKQKREEKAVVHAHVHEMVAMSHISGFNN
                     EARLNELLMGMHTETAYFIPEGMGYVPLLEPGSQELAKATLKSLKDHKVILWEKHGCL
                     ATGKDVDEAFDRIDLVAKAAKIYLLASHNRHIPS"
     gene            complement(1082..1531)
                     /locus_tag="PKZ74_11345"
     CDS             complement(1082..1531)
                     /locus_tag="PKZ74_11345"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPM93391.1"
                     /translation="MFSDEYFMREALKEARKAFDLDEVPVGAVIVCNDRIIGRGHNLT
                     ERLTDVTAHAEMQAFTAAAGHLGAKYLDECTLYVTLEPCVMCAGAAFWTQIGRIVYGA
                     PDDKRGYTKTGAGLLYPRTTVTPGILAQDCADLLIEFFKSKRNDATE"
     gene            complement(1565..2905)
                     /gene="nhaA"
                     /locus_tag="PKZ74_11350"
     CDS             complement(1565..2905)
                     /gene="nhaA"
                     /locus_tag="PKZ74_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPM93392.1"
                     /translation="MMFYKKMKDREKFHFSNFNISSYIREESLGGFLLLLTAVIAVIW
                     ANSGFADAYDKLWHEIKLGISFGDFQMKYSLQHWINDGLMAVFFFMVGLEIKRELIAG
                     ELSTIKKASLPIAAAAGGMLFPALIYVAFNAGNPEHLSGWGVPMATDIAFALGILSLL
                     GSRVNINLKIFLTALAIADDLGAILVIALFYTDTINIMQLVNAGIFLVILMVSNRFGV
                     RRTSYYAIVGFLGVWISFMFSGVHATIAGVLIALTIPVKTKITEKEYVDELCVLVDKF
                     ERENPNDKDLLTQRQSHLVSKIGKLSDDANTPLQKLEHALHPVTAYIILPLFALANAG
                     VKIEGNILDMMFHPVALGIIAGLAVGKILGISLFSRLMVRLKLAELPEGVSWRQVYGA
                     GMLAGIGFTMSIFISGLAFKSEELIQIAKVGIFTASILSALVGMIFLYSSKTKK"
     gene            complement(2963..3400)
                     /locus_tag="PKZ74_11355"
     CDS             complement(2963..3400)
                     /locus_tag="PKZ74_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPM93393.1"
                     /translation="MGEIKTKPTESDVKLFIDSVENEKKRSDSYAILEMMSDVTGEKP
                     VLWGTSLIGFGKYHYKSKSGQEGDWFLTGFSPRKQNISLYLMAGYVRGEDEVFDDLMN
                     KLGKYKTGAGCVYINKLEDINVLYLKELIRLSVNFLKNKYPEI"
     gene            3536..>5614
                     /locus_tag="PKZ74_11360"
     CDS             3536..>5614
                     /locus_tag="PKZ74_11360"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HPM93394.1"
                     /translation="MKKLFFAILLVLLVNAGFSQARIQVIHNSADAALETVDIWLDET
                     LLLDNFSFRTASAFTDAVPGNVSIVVAEADSQNPDNPLWSGNVELTDNETYVIVAEGM
                     ISQTGYNPYVPFDLAVYSGAREIATSEEMTDMITHHGSTDAPDLDINEIAIGLGTIVD
                     NIGYSEFDGYLELATKDFMFDVTEAAGSPLIDTYTAYFRILGFKGMAITVLTSGFLDP
                     AGNSNGPAFGLWVATAAGGDLIELPPYNPTARVQIIHNSPDAAASTVDVWMNDTRIAD
                     DLAFRTATPFLDLNAEEEITITVCEAGSENPSDPLWSGNYTLTVNEKYILVADGIISP
                     SGYDPAPPFDLELFPQAREVAFFPDNTDVLIHHGSTDAPIVDIYEVGTGAGLLADNLA
                     YTGFAGYLELEPLDYIIEIRDESGTNKIVAYELPLETIGLDGFAISLIASGFLYPENN
                     SNGPAFGLWMATAEGGNLIKLTVYDPKARLQFIHNSADNAAEMVDIWMNETLLVDNLE
                     FRTASAFLDVPALQELTITVAGPDSQSPDDPVWANTYNFEKDKSYLFVADGIVSQSGY
                     EPAVPFDMLVYPNAREEASSGGQTDMLIHHGSTDAPTVDLVEVGIGLGVMVDNLSYGE
                     FAGYFGLATVNYIIQVRDETGMTKIAAYSVPLGNMGLQGEAVTVVASGFLVPDNNSGG
                     PEFGLYLAKAS"
BASE COUNT         1409 a         1442 c         1346 g         1417 t
ORIGIN      
        1 cctggctcag ggaacagctc gcctccatcg gagccgaaac gccaatcatt ataagcctcc
       61 acatcccgct gctgacaacc gaagcacaaa ttctaagggg agccacggca gccaacgacc
      121 gaaacgaggt cgtcgtcaat tccaaagagg tgatcgatct ttttaatggc cacaacctga
      181 agctggtttt acagggccat ctgcattatt atgaatctct gcacgttttc gggacgaact
      241 atgtgacggc tggcgctgta tcggcagcct ggtgggaagg gccgttcctg ggcaccgagg
      301 aaggctttct actgctgaaa gccaaagggg atgagatcag ctgggaatat gtcgattatg
      361 gctggcaagc aggtaaatct tcgccgcttt agccaccagg tcgatccggt cgaatgcctc
      421 atcgacatcc ttgcctgttg ccaggcagcc atgcttttcc cagagaatga ccttatgatc
      481 tttcagcgat tttaaggtgg ctttggccag ctcctgcgat ccgggctcca gaagaggcac
      541 atagcccatt ccttccggga taaaataggc cgtttcggta tgcataccca tcaacaattc
      601 attcaaccgc gcttcattat taaatccgct gatgtgcgac atcgccacca tttcatgtac
      661 atgggcatgc acgactgctt tttcttcccg tttttgtttc accaggaggt tgtgaatagc
      721 aaagtgggag ggcagttcgg aagaggggac caggggattt tcagggtctt cgaacagtac
      781 ctggtacgcc tcacccgatt tggagatttt gaccaggcaa attccggaac cggggtcttt
      841 tgccagtgtc ctcatcctgg atcctttgct ggtaatgaag atgtagttgt gggcaatggc
      901 cggatatttt accggcaggg ggatcatcgg gtacgtcctg aaatcaatcc tgacattggg
      961 ataaaactcg gtgacattga ccgaaatatt tcctccgctg gcttccgccc agcccttttg
     1021 ccagagcagc gatgccactt cggcaattcc ggcggcgaca ccctgcgccc cctggttggt
     1081 cttattcagt tgcatcgttc cgtttggatt tgaaaaattc gatcagtaag tcagcgcaat
     1141 cctgcgcgag aatgcccggt gttactgttg tcctggggta cagcagaccg gcgccggttt
     1201 tggtgtagcc ccttttatcg tcgggcgccc cgtagacaat cctcccgatt tgtgtccaga
     1261 aagcggcccc tgcgcacatt acacagggtt ccagggtgac atacagggtg cattcgtcaa
     1321 ggtatttcgc gcccagatgg ccggcagcag cggtaaaagc ctgcatctcg gcatgcgccg
     1381 tcacatcggt cagcctctcg gtaaggttat gtccgcggcc gatgatccgg tcgttgcaaa
     1441 caatgacggc acctaccggg acttcgtcga gatcaaaagc tttccgggcc tctttcaagg
     1501 cttccctcat aaaatattca tcggaaaaca ttatctaaaa ttaaataata atgatgaatc
     1561 tgacctattt ttttgtcttt gaggagtaaa ggaagatcat tccgaccagt gccgatagaa
     1621 tggatgcagt gaaaatacct actttggcta tctgaataag ttcttcgctt ttaaaagcca
     1681 ggcctgaaat aaagatagac atagtgaacc ctatcccggc cagcattcct gcgccataaa
     1741 cctgtctcca tgaaacacct tcgggtaatt cggccagttt cagcctgacc atcagccggg
     1801 aaaacaatga aatccctaaa atcttaccca ctgccagacc ggctataatg ccaagggcaa
     1861 ccggatgaaa catcatgtca aggatgtttc cttcgatttt cacccctgca ttggccaaag
     1921 caaatagggg aaggatgata taggccgtta cagggtgaag agcgtgttcc agtttctgaa
     1981 gcggagtatt tgcatcatcg ctgagcttgc cgatttttga caccaggtgc gactgccttt
     2041 gcgtgagcag atccttatca ttaggatttt cccgttcaaa cttatcgacc aggacacaca
     2101 attcgtcgac gtattccttc tctgtaattt tcgttttaac cggaatggtc agggcaatca
     2161 gaacaccggc tatcgtcgca tgtacaccgg agaacataaa cgaaatccaa actccgagga
     2221 atccgactat ggcataatag gaggtccttc gcacaccaaa ccggttggaa accattagaa
     2281 tgaccaggaa aatcccggcg ttgaccagtt gcatgatatt gatcgtatcg gtatagaaaa
     2341 gggcgatgac caggatagct cccaggtcgt cggcaatggc aagggcagtg aggaaaatct
     2401 tcaggttgat gttcaccctg ctcccgagaa gggaaaggat cccgagtgcg aaagcaatat
     2461 cggtagccat gggaacgccc cagccggaga ggtgttccgg attgccggca ttgaatgcaa
     2521 catagatcag cgctgggaat aacatgccgc ctgcggctgc ggctatgggt agtgatgctt
     2581 ttttaatggt cgatagttcc ccggcaatca attccctttt tatctccagc ccgaccataa
     2641 agaagaacac agccatcagc ccgtcgttga tccagtgctg aagtgaatat ttcatttgaa
     2701 aatccccgaa agatattccc agttttatct catgccataa tttatcataa gcatcagcaa
     2761 atcccgaatt ggcccagata acggcgatta cagcagtaag aagtaaaagg aatccaccca
     2821 gcgattcttc acggatgtaa ctggaaatat taaaattgct gaaatggaac ttttcgcgat
     2881 ccttcatctt tttataaaac atcatcttta ctatcaaaaa taatgaagaa ttctgatttg
     2941 gcgggatgta aaaccatcca tattatattt caggatattt gtttttgaga aaattgaccg
     3001 acaaccggat caattctttc aaatacaaca cgttgatatc ctccagcttg ttgatataaa
     3061 cacaaccggc ccctgttttg tatttgccca gtttattcat caggtcgtca aatacttcat
     3121 cctcgcccct gacataaccg gccatgaggt acagtgatat gttctgcttc cggggtgaaa
     3181 aaccggtcag gaaccagtcg ccttcctggc cgctcttgga tttgtaatgg tattttccga
     3241 atccgatcag cgaagtgccc cacaatacgg gcttctcacc ggtaacatcg ctcatcattt
     3301 ccaggatcgc gtaactgtcg ctcctttttt tttcattttc taccgagtca ataaatagtt
     3361 ttacatccga ttcggtaggt tttgtcttta tttctcccat aacggtaaaa ttgtatgtta
     3421 ttaaaccaaa tttgttagtc attgttcaaa tatggagaaa aattgcattc cgacttatcc
     3481 tatatttgta aaaataaatt ttgaagatat ttgaaatgtt ataatccgtt ataaaatgaa
     3541 aaaactattt tttgctattc tactcgtact gcttgtcaat gcaggatttt cacaggccag
     3601 gatacaggtc atccataact cggccgacgc tgcactcgag actgttgata tctggctgga
     3661 tgaaacgcta ttgctggata atttttcatt ccggacagcc tctgcgttca cagatgccgt
     3721 tcccggtaat gtgtcgattg tcgtggccga agccgacagc cagaatcccg acaaccccct
     3781 ctggtcggga aacgttgaac tgactgataa tgaaacttat gttatagtgg ccgaagggat
     3841 gatcagccag accggttaca atccgtatgt cccgttcgat ctggctgttt acagtggagc
     3901 ccgggagata gcgacatccg aagagatgac cgatatgatc acccatcatg gttccaccga
     3961 cgcccctgac ctggatatca atgaaatagc cattggactt ggcactatcg tcgacaatat
     4021 cggatattcc gaatttgacg ggtacctgga actggctacc aaagatttca tgttcgatgt
     4081 gacggaagct gccggaagcc cgctgatcga cacctatacg gcctatttcc gcatccttgg
     4141 attcaaagga atggccataa ccgtgctgac ttccggcttt ctcgatccgg ccggcaacag
     4201 caacggcccg gctttcggcc tgtgggttgc cacggctgcc ggcggtgacc tgatcgagct
     4261 tcctccctat aatcctaccg cccgtgtgca gatcatccat aactcccccg acgcagcagc
     4321 ttcgactgtc gatgtctgga tgaacgatac ccgcattgcc gacgacctgg cgttccgcac
     4381 ggcaaccccg ttcctggacc tgaatgcgga agaggaaatt accatcaccg tttgtgaagc
     4441 gggcagcgag aatccgtccg acccgctatg gtcggggaac tataccctga cggtcaatga
     4501 aaagtatatc ctggtggccg acggcattat cagcccttcg ggatacgatc ctgcaccgcc
     4561 tttcgatctg gaattattcc cccaggcccg tgaagtagct ttcttccccg ataataccga
     4621 tgtccttatc catcacggtt caacggatgc tccaattgta gatatctatg aagtgggcac
     4681 cggggccggc ctgctggccg acaacctggc atacaccggt ttcgccggtt atctcgaact
     4741 tgaaccgctg gactacatca tcgagatcag ggatgaatcg ggaaccaata aaatagtcgc
     4801 gtacgaactt ccgcttgaaa cgatcggcct ggatggtttc gccatcagcc ttatcgcttc
     4861 cggattcctt taccctgaaa acaacagcaa cggtcccgcc ttcgggttgt ggatggctac
     4921 cgccgaaggg ggaaacctga tcaaactgac ggtctatgat ccgaaagcca ggctgcaatt
     4981 catccataac tcggccgata atgccgccga aatggtagat atctggatga atgaaacact
     5041 gctggtcgac aacctcgaat tccgcaccgc atcagcattt cttgatgtac cggcattgca
     5101 ggaactgacg atcaccgttg caggccccga cagccagagt ccggatgacc cggtttgggc
     5161 aaatacctat aatttcgaaa aagacaaatc ttaccttttt gtggccgatg gtattgtcag
     5221 ccagtcgggt tatgaaccgg ccgtcccctt tgatatgctt gtctatccca atgcaaggga
     5281 agaagccagc agcggcggac aaaccgatat gctgatccac cacggttcaa ccgacgcgcc
     5341 gaccgttgac ctggtagagg taggtatcgg ccttggcgtt atggtagata atctctccta
     5401 cggtgaattc gccgggtatt tcggcctggc caccgttaat tacatcatcc aggtgaggga
     5461 tgaaacgggg atgacaaaga ttgccgctta ttcagtcccg ctgggcaata tgggcctgca
     5521 gggcgaagcg gttacagtcg tggcatccgg cttcctcgtg cccgataaca acagcggcgg
     5581 ccccgaattc ggactatacc ttgctaaagc ttcc
//
LOCUS       DAOPNH010000232         5612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16725_length_5612_cov_4.452042, whole genome shotgun sequence.
ACCESSION   DAOPNH010000232 DAOPNH010000000
VERSION     DAOPNH010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16725_length_5612_cov_4.452042"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="PKZ74_11365"
     CDS             <1..1939
                     /locus_tag="PKZ74_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93395.1"
                     /translation="FQIWPYTNSGLYIDYKTDGTVPLTNGWTDFMDFLATANGNWNSP
                     ATWSVSLPNGGGWTPATTEIPIYKTSDVYISDNFTVIYPPSYNAGKVKTLRVYGTGTL
                     KANSSSGSCYLYVYGDIMVDGVIGGPTDVLGLDIEGSNCNVYGSNSLVVSRIAKYTTH
                     SATTNLVLVRPLTLTYSHATNPALWNQNPATTTFNILVVDHAPVTINNANVDLNGCNF
                     YLNYPSSAILKSVSGSGTMTSYVTIPSWTDDAHGWLLLSSPMTSQSVSPAFTDAVPAN
                     YDFFAWDEVNDMWLNQKVAGNNITSFVPGHGYLAAYQNTPGRNFTGQVTTTDVTVNNL
                     TMSGGTYSGWNLLGNPYNSSLKWNDGTIWTVPATIAATAKVWNEPTAAYVDIDPGENI
                     PAFNGFMVQVLSGSPASLTIPVAARVHDFLSIYKDVPGNLKLVAYDRTGNTAQECAIG
                     ANDQATGGYDRDFDSRFLAGYAPKFYALAGGEMLSTHIVPALNTASAIDLGFEKNGQT
                     EFSIGLENAIPGLTVYLTDKKTGITTELKDGVEYSFISLEGDEPNRFLLHFSPLSIED
                     PGNVNPFEVYSTGDQIRILSHAEGESELYVTNLVGQVVLAGRAGEGMTTVNAASLTNG
                     VYLVNLASSKGIVSHKVIINK"
     gene            1994..2221
                     /locus_tag="PKZ74_11370"
     CDS             1994..2221
                     /locus_tag="PKZ74_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93396.1"
                     /translation="MKNRFKILALGLTLGFIIGLSNISIPQPPPPPDHGDPGDVPGGT
                     APVGSGLVLLIGMGAAYGSRKLYRAWKNIEE"
     gene            2400..2796
                     /gene="ssrA"
                     /locus_tag="PKZ74_11375"
     tmRNA           2400..2796
                     /gene="ssrA"
                     /locus_tag="PKZ74_11375"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2987..5092
                     /locus_tag="PKZ74_11380"
     CDS             2987..5092
                     /locus_tag="PKZ74_11380"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPM93397.1"
                     /translation="MKSLSILIPVFFMATLAAAQWNQVSPYPTIGNLFGSAFFVDEYE
                     GWVYKSGLFHTSDGGASWEKQLSDCTQEPLECLYFVNADEGWGAGLGTIMHTSDGGGS
                     WTVQFSGQDVVFESLHFIDEMHGWAVGYNFDGSTGWTWSELLETTDGGVNWNDVYIDR
                     TDMQGAYHDIFFTDSSHGWLLATINNNGYLKRTTDGGTTWTSQSFFSYMDQVFFADTL
                     HGWIINDSEIYHSSDGGATWVQQNAGQAEGLSEIHFSSPQKGMITGYDGLLLKTDNGG
                     QNWQATFITSPEYPYDRLDYLCVPDSLNCWVVGSLGGIYHSNDAGDSWELQSRKITNS
                     FINAIYFTDVENGWMVGNNGTVVGTADEGATWQTLGFGDGTLFDVTFTDADHGWIVGS
                     AGTILHTVNGGSDWDYQASPTTGTLYDVCFVNNLRGWIGCDNGILLHTFDGGLTWTEQ
                     DCGVEDYSDIKSVCFTDENNGWIALYSIPGELLHTNDGGQTWISQFTDNSARFNSIFF
                     TDSLNGWAAGNKVVHTGNGGLTWEVQLPLSVYQYYEVMFIDPNTGWIGPPLRYTLDGG
                     LTWKSYTCLEGPSDLWSFYFLDELNGWGAWADAVFHTTTGGFADLPERERITSIKHFC
                     YPNPFPEKIEICFNVGRPEKTGIQILDMMGKQVYRLIYDSTEGENIISLDLNALSKGL
                     YVYRIATKEKSVTGKIIKL"
     gene            complement(5215..>5612)
                     /locus_tag="PKZ74_11385"
     CDS             complement(5215..>5612)
                     /locus_tag="PKZ74_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93398.1"
                     /translation="FRKIVRVCFFFFPVIIAFVVFMYRSFIYMQKRAIKTKEKILSTA
                     RKLFAANVSRSLSPGGIWASLIGSTEGAARETGPPRRTASEVIASVEPYLEIVELKSV
                     LFRTNFPNAPKAWLCVSRQRKEPAQPSGR"
BASE COUNT         1434 a         1380 c         1386 g         1412 t
ORIGIN      
        1 tttccagatt tggccctata cgaattcagg attatacatc gattataaaa ccgatgggac
       61 ggttccgctg acaaatggat ggaccgattt tatggatttc ctggctacag caaacggtaa
      121 ttggaattca cctgctacct ggtcggtcag tttacccaac ggtggtggct ggaccccggc
      181 tacgacagaa attccgattt ataagacttc agacgtttat atcagcgata atttcacagt
      241 gatctatcca ccgtcctata atgcggggaa agtcaagacg ctgcgcgtat atggtaccgg
      301 tacgctgaaa gcaaattcat cttcaggaag ctgttatctc tatgtatatg gcgatatcat
      361 ggtcgatggg gtaatcggcg ggccaaccga tgtgctcggg cttgatatag aggggtcgaa
      421 ttgtaacgtc tacggcagca acagccttgt ggtatccaga atcgccaaat acacaactca
      481 ttcagcaaca accaacctgg ttcttgtccg tcccctgaca ttaacctatt cacatgccac
      541 caatccggca ctatggaatc aaaaccctgc cactaccacg tttaatatcc tcgttgttga
      601 tcacgcaccg gtaaccataa acaatgccaa tgtggacctc aacgggtgca acttttatct
      661 gaactatccg tcatccgcta tcctgaagag tgtgagcggt tcaggcacaa tgacatcata
      721 tgttactatt ccttcatgga ctgatgatgc gcatggctgg ctcctgctct cttcaccgat
      781 gacttcgcag tcggtctctc cggcattcac cgacgcagtg ccggctaatt atgatttctt
      841 tgcatgggac gaagttaacg acatgtggct taaccagaaa gtcgccggaa ataatattac
      901 cagtttcgtt cccggccatg gctaccttgc tgcctatcag aacactccgg gcagaaattt
      961 tacgggtcag gttacaacta ctgatgtcac tgtaaacaac cttactatga gtggcgggac
     1021 ttactcaggt tggaacctgc tgggaaatcc ctataacagt tccctgaagt ggaacgatgg
     1081 tacgatctgg acagtgccgg ccaccattgc cgctacggca aaagtctgga atgaacccac
     1141 tgcagcctat gtcgatattg atcccggtga aaacatcccg gcattcaatg gttttatggt
     1201 acaggtgctc tctggctcgc ctgcgtccct gaccattcca gtcgccgcaa gggtacatga
     1261 tttcctgtct atttataaag acgttccggg aaaccttaag ctggtcgcct acgaccggac
     1321 gggcaacacc gcccaggaat gcgccatcgg cgccaatgac caggccaccg gcggatacga
     1381 ccgcgacttc gactcccgct tcctggccgg atacgcaccg aagttctatg ccctggccgg
     1441 cggcgaaatg ctctctaccc acattgtgcc ggcactcaac accgccagcg ccatcgacct
     1501 gggatttgag aagaacggac aaaccgagtt cagcatcgga ctggaaaatg ctatcccggg
     1561 ccttaccgtt tatttgaccg ataaaaagac cggtattacc accgagctta aagacggcgt
     1621 cgagtactcc ttcatttcac tggagggtga tgaacccaac cgtttcctgc tccatttcag
     1681 cccgctgagt atcgaagatc cgggcaatgt aaacccgttc gaggtctatt ccacgggcga
     1741 ccagatccgc atcctgtcgc atgccgaagg ggagagcgag ctgtatgtca ccaatctcgt
     1801 ggggcaggtc gttctggccg gacgtgccgg tgaaggaatg acaacggtga atgccgcttc
     1861 gctgaccaac ggtgtatacc tggtgaacct ggcatcttcc aaaggtattg tcagccataa
     1921 ggttatcatc aataagtagt aagatatgga aggaaagata aacgagagat caacacaaga
     1981 aaaaagaatg actatgaaaa acagattcaa gatactggca ctgggtttga cccttggatt
     2041 tattatcgga ctgagcaaca tcagcattcc tcagccgccc ccgccacctg atcacggaga
     2101 cccgggagat gttcccggcg gaacggcccc ggtgggaagc ggactggtgc ttctgatcgg
     2161 catgggtgcc gcctacggct cccggaagct ctacagagcc tggaagaaca ttgaagaata
     2221 gaccggtata gacccgtctg attcacatcc ccgtcggcca gaatcctggc cggcggggat
     2281 tttatttacc ccgacatcaa aaaaaccacc gaaaatccct atttttgcac ggtatttgtg
     2341 caatcccaca tccgttcttt gtaaaattaa cacataacac aaaactcaac attcaatttg
     2401 gggctgccag gtttcgacag caggtttgtg gttgagtaag catgccgagc cttgcagata
     2461 cgctcgtaaa tatcatctgc gaaccaataa ctggcgaaaa taacttcgct cttgctgcct
     2521 aatctgcgta tcgcggaggt ggcttaatcc cgactcaagg agtcgggaca ggacgttctg
     2581 ccggcggatc tgcctgattc caaccggacc acagggcgct cgcaatttcg ggctggtcat
     2641 ggcgaagctc cggcgtcgtg gcgagataaa ggagataagg gtgggccttg ggtgccgttt
     2701 tccgaacccg ccccgacaat caacaagcgg ataagcatgt agaaagcgcc tctgctatct
     2761 gtttggacga gggttcgact ccctccagct ccaccttcgc ccgccgaagc attgcgaaca
     2821 aggcaaagct tcggcctttt tatttcatcc ctggccattg tcattgttcc tgtgaaataa
     2881 gaaatgccgg ctcaggaggc tatgaaattt caacagttgt cgtgcaacct catcaagttc
     2941 tttcttgtct tatgtttgtg atacgattcc tgcaaattgc ccgatgatga aaagcctttc
     3001 gatcctgatc cccgtctttt ttatggccac cctggcggca gctcaatgga accaggtaag
     3061 cccttatcct actattggca atctttttgg aagtgccttc ttcgttgatg aatatgaagg
     3121 ctgggtatac aaatccggac tttttcatac ttccgacggg ggggccagct gggagaaaca
     3181 actttcggat tgcacgcagg aacctctcga atgcctttat tttgtgaatg cagacgaggg
     3241 ttggggtgcg ggacttggaa ccattatgca tacttctgat ggtggtggta gctggactgt
     3301 tcagttctca ggccaggatg tggtgtttga atcgcttcat tttattgatg aaatgcacgg
     3361 atgggctgta ggatacaatt ttgatggaag tacaggatgg acatggtctg aacttctgga
     3421 aaccaccgac gggggcgtta actggaatga cgtttatata gatcgaacgg atatgcaggg
     3481 agcctaccat gacatttttt tcactgatag cagtcatggc tggttactgg ctacaataaa
     3541 taataacggt tacctcaaac gaacaactga cggaggcacg acatggacca gccagagctt
     3601 ctttagctat atggaccagg tgtttttcgc tgatactttg catggatgga tcattaatga
     3661 ttctgagatc tatcattcct cagacggcgg agcaacctgg gttcagcaaa acgcaggcca
     3721 ggccgagggt ttaagcgaaa tacatttctc ctccccgcaa aaaggcatga tcacaggcta
     3781 tgacggactt ttactgaaaa cagacaacgg aggccagaac tggcaggcca ccttcattac
     3841 aagccccgaa tacccgtatg acaggctgga ttacctttgc gtgcccgaca gcctgaactg
     3901 ctgggtcgtc ggtagccttg gcggcatcta tcattcaaac gacgcaggtg attcgtggga
     3961 gttgcagagt cgcaaaatca ccaattcgtt catcaatgcg atatatttta cggatgtgga
     4021 gaacggctgg atggtcggaa acaacggcac tgtcgtgggt actgcggatg agggggctac
     4081 ctggcagact cttggctttg gggacggaac tttattcgac gtaacattta ccgatgctga
     4141 tcatggctgg atcgtcggat ctgccggaac gatcctgcat accgttaacg gtggttccga
     4201 ctgggattac caggccagcc cgacaacagg aactctttat gatgtgtgct ttgtgaataa
     4261 cctccgggga tggatcgggt gcgacaatgg aattctcctg catacctttg acggagggct
     4321 gacatggact gagcaggatt gtggcgttga agattacagt gacattaaat cggtatgctt
     4381 taccgatgaa aacaatggat ggatagcgtt gtacagtatt ccgggtgaat tattgcatac
     4441 aaacgatggc ggacagacct ggatttctca gtttacggat aattctgccc gtttcaattc
     4501 catcttcttt acagatagcc ttaatggctg ggccgcagga aataaagtgg tgcataccgg
     4561 caacggtgga ctgacctggg aggtgcaact tccgttaagc gtataccaat actacgaggt
     4621 catgtttatc gacccgaata cgggttggat cggcccgccg ttgcgatata cgctggatgg
     4681 cggcctgacc tggaaatctt acacatgcct tgaaggtccg tcggatctct ggtcttttta
     4741 ttttctggat gagctgaacg gttggggcgc atgggctgat gccgttttcc atacaaccac
     4801 cggtggcttt gccgacctgc ctgaaagaga aaggatcaca tcaataaagc atttctgcta
     4861 cccaaaccct ttccccgaaa agatcgaaat ctgttttaat gtcggccgtc ctgaaaaaac
     4921 agggattcaa attcttgata tgatgggaaa acaggtttat cgcctgattt atgactccac
     4981 ggagggtgaa aacattattt cacttgattt gaatgcatta tcaaaaggat tatatgttta
     5041 caggatagcc acgaaagaaa aatcagttac cggtaagatt attaagttgt aggtttattg
     5101 gctataagtc ataagtcgtt agtcatcact aattacccca ttgctaatta acccattacg
     5161 cattaataca ttactcatta aacattaagc attaaacatt aattgaacat tcccttacct
     5221 gcccgacggc tgtgccggct cttttcgctg tcttgatacg caaagccagg ctttaggggc
     5281 attcggaaag tttgtccgga agagcactga ttttaattct acaatctcaa ggtacggctc
     5341 aacagaagcg atgacctcac tggctgtccg gcgtggtggt ccggtttccc ttgcagcgcc
     5401 ttccgtgctg ccgatcaggc tggcccagat ccctcccggc gacagtgaac ggctcacatt
     5461 ggcggcaaaa agcttgcgcg ccgttgaaag gatcttctcc ttcgtcttta tcgctctctt
     5521 ctgcatatat ataaaactcc tgtacataaa taccacaaat gcaattatta caggaaagaa
     5581 gaaaaaacaa acacggacaa tctttctgaa ga
//
LOCUS       DAOPNH010000233         5591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16815_length_5591_cov_7.135296, whole genome shotgun sequence.
ACCESSION   DAOPNH010000233 DAOPNH010000000
VERSION     DAOPNH010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16815_length_5591_cov_7.135296"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKZ74_11390"
     CDS             <1..694
                     /locus_tag="PKZ74_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93399.1"
                     /translation="PNVQNVPLFNSLNQANFTAGYRFGTYSRGWDIQSAYSITDHIGV
                     MGNYQHFGAQYTEWNNGTAVFDNGHFKGDYFEFGAGYYTLIPKEFIFESYAGTGWGKV
                     TNEFSYQFYPVESAVNYNRYFVQPSIGWRHSHIGLAFSTRFCVVHYTRYKMIDPQNTY
                     DINELMGLEHNPCWLLEPAFTFRGGGRIVKFQVQACLSFPLNNKEINYDPFSFSFGVV
                     AMINGKTGRKEK"
     gene            823..1524
                     /locus_tag="PKZ74_11395"
     CDS             823..1524
                     /locus_tag="PKZ74_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93400.1"
                     /translation="MISGCSQNYYVHNTVNVPAFKEKNEAQLAVSYGFSYYESYNLDV
                     QAAHAPFNHLAYMVNYHHYWDDLGENESYQGDFVEFGPGYYKCWADEFVFETYGGVGW
                     GWITNKFELDGENTTSKINYNRYFIQPSVSGRWDGEDWGNMFAKFSIAARFSILDYNT
                     MRLEPENNNYSMSTIIGFASNPTYLIEPGGGLTIGWKYLSVLIQGSIPILLNNYDMKI
                     EDSFHINVGLVGIIH"
     gene            1611..2453
                     /locus_tag="PKZ74_11400"
     CDS             1611..2453
                     /locus_tag="PKZ74_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPM93401.1"
                     /translation="MRWQGRRTSGNVEDRRGMSGGKLAVGGGLGTLVIIVIVYLLGGN
                     PGDIMNQLQLGGQEQTTMTTAEEDQLAEFVSVVLADCEDVWTKIFTESGQSYRKPKLV
                     LYTSQVNSACGMAGSASGPFYCPLDEKIYIDLSFMEDMERKLNAPGDFAMAYVIAHEV
                     GHHVQKMLGILGQVQELRGQVSEQEYNEYSVRLELQADFLAGVWAYHAEEMFGILEQG
                     DIQEALGAANAVGDDRIQMQSQGYVVPDSFTHGTSAQRMKWFNKGFNTGDISQGDTFN
                     QENI"
     gene            complement(2493..2885)
                     /locus_tag="PKZ74_11405"
     CDS             complement(2493..2885)
                     /locus_tag="PKZ74_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93402.1"
                     /translation="MKTFGIILAIIVLVVLVAGTFLYLGIYDEGVRAGTVVRVSKKGV
                     VFKTYEGQLNLQSFGALKNASPFAETFDFSVEKSKPEVVRELEAVALSGERVNLHYVK
                     RYMKFPWRGETKYFITSVEREGERPFEE"
     gene            3129..5207
                     /locus_tag="PKZ74_11410"
     CDS             3129..5207
                     /locus_tag="PKZ74_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM93403.1"
                     /translation="MKKFLLIPVFLMISFYSAKSSGVCIVDAYQGIYFRLIANHVSVL
                     VNDQIATVVTTQEFRNLTGADHVIKYAFPLHEEASATNIRWKLGGVWYTAVIAPEPQD
                     TTLPGGGNDPDPDLVDHLGETPLYFTLLDTIPDDSTLVIELTYVQLLPYEYSKVDFFF
                     PNNYELIQTFPLNFQSLDFRLYSQRTIEDIQLFSPSGGVIGNYGDSATIFYEITEVAA
                     DFDYSAQYELNSDELGLFAFSTFFPDSMVTCDDYGNGFFTFIVEPDPNDSLNIIQKVF
                     TLIIDRSGSMTGIKMVQAKEAANYIINNLNEGDYFNIVDFETNVSSFQPDHVPFTPEN
                     QQAALDYIYPMYASGSTNISGAFGEAIPDFAGSDSTIANIIIFFTDGQATSGIIGTDQ
                     ILEHIQNLISYNEVSNLSIHTFGIGADVNYSLLSQIASQNNGLCHFLENEELLEAITE
                     FYNMIRNPVLLNPVMTIDPPVIVETYPNPLTNLYIGHQLIITGRYDTAVPVTVTFSGE
                     AYGIQQEYIYNIDLADTVISQNQFLTKLWAKQKMEYLYVLYFTFDEESPEAEEIKNLI
                     IDISICYNVISPFTNYTGGGGTTTVEEDAGTQPDAQRICRFYPNPFSTSGTFNLLVEE
                     DYSGTAFLEIFDNTGKVVMILEIEINGQGNYEIRWDGKDNSGNPLKPGIYLFTVTFGD
                     KSYSGKIFKM"
     gene            complement(5234..>5591)
                     /locus_tag="PKZ74_11415"
     CDS             complement(5234..>5591)
                     /locus_tag="PKZ74_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93404.1"
                     /translation="TNPNGCNPARSAEYSSSYSNVATNAVLGVDDPNRELSISTYPNP
                     VKDKLTITTGEMLKGEVRVSISDLLGHTVYSENIQDPGSNGSFTVNTSDLKEGIYMIK
                     ISAENGQVTRKIVVNR"
BASE COUNT         1520 a         1326 c         1279 g         1466 t
ORIGIN      
        1 tccgaatgtg cagaacgtcc ctttgttcaa cagcctgaac caggcaaatt tcacagcagg
       61 ttaccggttt gggacctact cgagggggtg ggatattcag tcggcttatt cgatcaccga
      121 tcacatcggg gttatgggca attatcagca tttcggggca caatatacgg aatggaacaa
      181 cggcacggct gtttttgata acggtcactt caaaggggat tattttgaat tcggggcagg
      241 ttattataca ctcattccca aagaattcat attcgaatcg tatgccggaa ccggctgggg
      301 aaaagtcacc aacgaattca gttatcaatt ttacccggta gagtcggccg tcaattacaa
      361 ccggtatttt gtccagcctt cgatcggatg gcgtcattcc catatcgggc tggccttttc
      421 cacccgcttc tgcgtagtac attacacccg ttacaaaatg attgatccgc aaaacacata
      481 cgacattaat gaactaatgg gtctcgaaca caatccctgc tggttgctgg agcctgcttt
      541 taccttcagg ggcggcggaa ggatcgtcaa attccaggta caggcctgct tgtcgtttcc
      601 attgaacaat aaagagatca actatgatcc tttcagtttt agtttcgggg tggtggcaat
      661 gatcaacggt aagacgggga ggaaagaaaa ataagtcagt atgaatgcca attacattaa
      721 agcatcaatc attactagtt ttatattaat ctaaaacaca atcattttat gagtgattat
      781 cataagtctg ttcggaaatc ttgctggatc atattaagta tcatgatttc cggttgttcg
      841 cagaattatt atgtgcacaa taccgtcaat gtacccgctt tcaaagaaaa gaacgaagca
      901 caactggctg tatcttatgg attctcctat tacgaatcat ataatcttga cgtgcaggcg
      961 gcacatgctc cctttaatca tcttgcctat atggtcaact accaccatta ctgggacgat
     1021 ttgggggaga atgaatccta tcagggagac tttgttgagt tcggacctgg ttattataaa
     1081 tgctgggcag atgaatttgt ttttgagact tacggcggag tcggatgggg atggataacc
     1141 aataaattcg aattagatgg tgaaaacaca acttcaaaaa taaattataa ccggtatttt
     1201 atccaacctt cagtcagtgg ccgatgggat ggtgaagact ggggaaatat gttcgcaaaa
     1261 ttctcaatcg ctgcaagatt ctccatcctg gattacaata cgatgcggct tgagccagaa
     1321 aacaataatt actcaatgtc gacaattata ggttttgcta gtaatccgac ctacctgatt
     1381 gaacccggcg gtggattaac gataggatgg aagtatctga gcgttctgat ccagggttcc
     1441 ataccaattc tgcttaataa ttatgatatg aaaatagaag attcatttca catcaatgtc
     1501 gggttggttg ggatcattca ttaacagaag ctaaaagtta gggacgccat ccgccagccg
     1561 gcggatggcg tcctcttttt tttattacct ttatctcaaa accaagttat atgcgctggc
     1621 aaggaagaag aacaagcgga aacgtggaag accgccgcgg gatgagcggt ggcaaactcg
     1681 ccgtcggtgg cggcctggga accctggtga tcatcgtgat cgtctatctg ctgggcggta
     1741 atccgggaga tatcatgaac caactgcaac tgggcgggca ggaacaaacc accatgacaa
     1801 cagccgaaga agatcaactg gcggaattcg tttcggtggt gctggccgat tgtgaagatg
     1861 tctggaccaa aatctttacg gaatcggggc aatcgtaccg gaaacccaag ctggtgctgt
     1921 acacttcaca ggtcaattcg gcctgtggca tggccggatc agcctccggg cctttttact
     1981 gcccgctgga tgaaaagatc tatatcgacc tgagctttat ggaagatatg gagcggaaac
     2041 tgaatgcccc gggcgatttt gccatggcct atgtcatcgc gcacgaggtc gggcatcatg
     2101 tacagaaaat gctgggcata ctgggacagg tccaggaact gagaggacag gtcagtgaac
     2161 aggaatacaa cgaatattcg gtacgactgg agctccaggc cgacttcctt gcaggcgtct
     2221 gggcgtatca tgcggaggaa atgttcggaa ttcttgagca aggcgatatc caggaggccc
     2281 tgggcgctgc caatgccgtc ggcgacgacc ggatccagat gcaatcgcag ggctatgtcg
     2341 tccccgactc atttacccac ggcacctctg cccaacggat gaaatggttc aacaaagggt
     2401 ttaacaccgg cgatatcagt cagggagaca cttttaacca ggaaaatatc tgatccaaac
     2461 cttattcaac cttcaacatt caaccttcaa cattattcct caaacggcct ctccccttcc
     2521 ctttccacac tggtgataaa atatttcgtt tcgcccctcc agggaaattt catgtagcgc
     2581 ttgacataat gcaggttcac ccgttccccg ctcagggcca cggcttccag ttccctcacc
     2641 acttccggct tgcttttctc tactgaaaaa tcaaacgtct ctgcaaacgg agaggcattt
     2701 ttcaacgcac cgaacgattg caggttcaac tgaccttcgt aggtcttgaa cacgacccct
     2761 ttcttgctta ccctgaccac ggtgccggcc ctgacgcctt catcgtagat gcccagatac
     2821 agaaaagtgc ctgctaccag cacgacgaga acaatgatgg ccagaattat tccgaaagtt
     2881 ttcatgaagt ccgattttgt gatactcatt ccggttaaca aaggtaattc aaatatctcg
     2941 taaaagccag aatggcaaac aggcaccggg aaactacagg aattcaataa aaatattaac
     3001 cggatgcaac tgtttttcca tgagaactgt cttattatcg atgggttcaa ggttgaataa
     3061 caaacagggt tgatccgggc tgattgaata atttgctgaa tattaaagcc attccaaata
     3121 ccctcaatat gaaaaagttc ttactaatcc cggttttttt aatgatttcc ttctactccg
     3181 ccaaatccag cggcgtttgc attgtggatg cataccaggg aatttatttc aggctgatcg
     3241 ccaaccatgt aagcgttttg gtgaatgacc agatagctac agtggtcacg acgcaggaat
     3301 tcaggaacct cacaggtgcc gatcatgtta taaagtatgc ttttccgctt cacgaagagg
     3361 ccagcgcgac caatatccgc tggaaactgg gcggggtctg gtataccgcc gttatcgcac
     3421 cggaacccca ggacaccacg ctgccgggcg gaggaaatga cccggacccc gacctggtgg
     3481 atcatctcgg agaaacacct ctctatttta ctttactgga taccatcccg gatgactcaa
     3541 cgctggtcat tgaactcacc tatgtccagc tgctgcccta tgaatacagt aaggtggatt
     3601 tctttttccc caataattat gaacttatcc agacttttcc gctgaatttt caatcgctgg
     3661 atttcagact ttattctcaa cgaacgattg aagacataca gctattttct ccttccggag
     3721 gtgtaatcgg gaactacggc gacagtgcca caattttcta cgagattacg gaagtggccg
     3781 ccgatttcga ttattcggcg caatacgaac tgaactccga cgaattagga cttttcgctt
     3841 tcagcacctt tttccccgat tccatggtga cctgcgacga ctacggaaac ggtttcttca
     3901 ccttcatcgt ggagcccgat ccgaatgaca gcctgaatat tattcaaaag gtgtttaccc
     3961 tgatcatcga ccggtcggga agtatgacag gtattaaaat ggtgcaggca aaagaggcgg
     4021 ccaattacat tattaacaac ctcaatgagg gcgattactt caatatcgtg gacttcgaaa
     4081 cgaacgtcag ctctttccag cctgaccatg ttccctttac acctgagaac cagcaggctg
     4141 cgctggatta tatctatccg atgtacgcca gcggttcaac aaatatttca ggcgcttttg
     4201 gcgaggcaat acccgatttt gccggaagtg attcaacgat tgccaacatc atcatctttt
     4261 ttacggatgg ccaggctacc tccggtatta taggaacaga tcaaatcctg gaacatatcc
     4321 agaaccttat ttcatataac gaggtgagca atctttctat tcatactttc ggaattggtg
     4381 cggatgtcaa ttactcatta ctctctcaaa ttgcttcgca aaataacggc ctttgccatt
     4441 ttcttgaaaa tgaagaacta ctcgaagcca ttaccgaatt ctacaacatg atccggaatc
     4501 cggtactgct caacccggtg atgaccatcg atccgcccgt catcgtggaa acttatccga
     4561 atcccctgac gaatctttat atcggccacc agcttatcat taccggaagg tacgatactg
     4621 ctgttcccgt taccgtaaca ttcagcggcg aagcgtatgg tattcagcag gaatatattt
     4681 acaacatcga ccttgccgat acggtgatca gccagaacca attcctgaca aagctctggg
     4741 ctaagcaaaa gatggagtac ctctatgtcc tctatttcac ctttgatgag gaaagtccgg
     4801 aagcagagga aattaaaaat ctgatcatcg atatcagcat ttgctataac gtgatcagtc
     4861 cgttcaccaa ctataccgga ggaggcggaa cgaccaccgt agaagaagat gccggaaccc
     4921 agccggatgc ccaacgcatc tgccggttct acccgaatcc attttccacg tcaggcactt
     4981 tcaacctgtt ggtggaagag gattacagcg gaactgcctt cctggaaatc ttcgacaaca
     5041 cgggaaaagt cgtcatgatc ctggagattg agatcaacgg tcagggtaat tatgagatca
     5101 ggtgggatgg aaaggataat tccgggaatc ctttgaaacc cggaatatac ctctttacag
     5161 ttactttcgg cgataaatct tatagcggga agatttttaa gatgtagaaa gaattgaatt
     5221 taccggcaaa gatttatctg ttaacgacaa tctttcgggt cacctgccca ttttcggcac
     5281 tgatctttat catataaatc ccttctttca agtcagatgt attgactgtg aaggaaccgt
     5341 tcgatccggg atcctgaata ttttccgaat agaccgtatg ccccagaaga tcactgatag
     5401 aaaccctgac ctcgcctttc agcatttcac ccgtcgtgat ggtcagttta tccttgaccg
     5461 ggtttggata ggtggagatg ctcagctccc tgttcggatc atccacaccc agtacggcat
     5521 tggtggccac attggagtaa gagctgctgt attctgcgct cctggccgga ttgcagccgt
     5581 tggggttggt c
//
LOCUS       DAOPNH010000234         5579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16858_length_5579_cov_8.280413, whole genome shotgun sequence.
ACCESSION   DAOPNH010000234 DAOPNH010000000
VERSION     DAOPNH010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_5579_cov_8.280413"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..765
                     /locus_tag="PKZ74_11420"
     CDS             61..765
                     /locus_tag="PKZ74_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPM93405.1"
                     /translation="MIKQILALANDLSGLFFPKLCLACGEHLLRGEDAICSICHFHLP
                     KTYFHNDPDNPLNKVFWGRVNLEGVASYLYFQKGSTVQHLLHLLKYKGKEEIGVRLGQ
                     WYGLDLKFAAPFRDAGLIIPVPLHPAKKRKRGYNQSEAFAEGLASVMTGRLETRALYR
                     RVNTKTQTRKSRYDRWENVENIFAVRNPEKLRYQHVLLVDDVLTTGATLEACARALLD
                     VPGVKVSVVTIAFASY"
     gene            complement(766..3378)
                     /locus_tag="PKZ74_11425"
     CDS             complement(766..3378)
                     /locus_tag="PKZ74_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM93406.1"
                     /translation="MKKHLLFFVLLPVCLAAQVRDDFSDGDFTTGPAWQGDLGHFTLS
                     YSSAVPENQRPALQLNAPAAGISSLRLAEPFSGDLEWQFWIKMSFNTSSSNFARIYLM
                     TDSPDPQQPLNGYFIQAGGTSDSVSFWRQDSAVFTELLRLDQGYTGNSTNTLRLRITR
                     DSEGNWKFFCNTAGGMSLQPAGECADTVFPEGNYYSIYCQYTSSNITKFYFDDIYAGP
                     LIIDSIPPVLLKAKALNPSIVLLEFSEPLDATAAGNPLNYLFDEGIGHPAEALCLADP
                     SQVLLNLANNLSSGTACSLTITGIRDVAGNEAPLVILPVLYYKTQPYDIIITEIMADP
                     SPPLDLPEFEYLELHNRSGFAIDLEGLSLGISTSLHDLPSVILNPGHFVILCSDDAYG
                     TLHYLAPAIAIPSFNLPNSGAELYLRDSSGLTVCYLQYDLSWYGDDAKDDGGWALEMV
                     DTGNPCAGRENWTASYAMEGGTPGKPNSAYATEPLPRTVLSCCLLSDTLLSVTFSESL
                     DSLSATDPAFYEVTPYTGAPVSVALSTPDHTTALLAFAVPFISRQSFTLSVKPGMANC
                     TGILSEEFTEKAFALPELAEPGEIVINEVLFNPKDDGVDYVELYNRSEKAINLENWLL
                     ASISTDPAGIADTQALTIIAGCRVMLPGEYLALCSDPGMVQNQYETPAPEAFLEMSSF
                     PSFNNDEGYVLLMDDNDQKIDGMRYSEEMHFLMLLSYDGVSLERISPERSADDATNWH
                     SAAETSGFGTPGYRNSQYLAAVPEETSAISVEPMVFSPDGDGKDDNLGIFFDFTSPGK
                     LLTIIIFNANGYFARKLADNELPGSSAFYSWDGTLDDRTPAPDGIYVILAEVLGMDGK
                     MNRYKKACVLARGR"
     gene            3479..4480
                     /locus_tag="PKZ74_11430"
     CDS             3479..4480
                     /locus_tag="PKZ74_11430"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPM93407.1"
                     /translation="MKICVVGATGLVGSEMVKVLGESGPGHIEFYPVASERSVGKMVS
                     FRGKEHPVIGMQEAVEKRADIALFSAGAAASRQWAPLFAAAGTTVIDNSSAWRQDPEI
                     PLIVPEINAHILNSSHKIISNPNCSTIQMVMVLAPLHRRFTIRRVVVSTYQSVTGSGA
                     KGYLQLENERKGIDGPKFYPHTIDLNVIPHGGDFTDNGYTTEEVKLDFETRKIIGDDS
                     IKVTATVVRVPVYGGHSESVNVEFEQPFETEEVLRILANEKGIAVIDHPAKAEYPMPI
                     TARGKDEVFVGRIRRDLTVANGLNMWIVSDNLRKGAATNAVQIAEYLLENKLVGLDM"
     gene            4557..5483
                     /locus_tag="PKZ74_11435"
     CDS             4557..5483
                     /locus_tag="PKZ74_11435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPM93408.1"
                     /translation="MKIYHSIDLFQPVPNPVVTVGTFDGVHRGHQAIFDAMRNEALRC
                     GGETVVVTFYPHPRLVIHPDSKSLRFINTQERKYEIISRSRIDHLIIIPFTREFSNLS
                     SGEFVRRYLVEKIKIHQLIVGYDHHFGKDRLGGYNELKGLGKIHGFEVSEVPAMIIDG
                     IPVSSTKIRNALIEGNIRLANSLLGYNYSISGKVVYGNRIGRTIGFPTANIEQEDEYK
                     LISAVGVYACLVDHHGRTYKGMGNIGYRPTIDVGSLTIEVNIFDFDEEIYGDRIIIYF
                     IERIRDEVKFENLTALRDQLVADRAKVLEILR"
BASE COUNT         1343 a         1415 c         1578 g         1243 t
ORIGIN      
        1 cttcgtgcgc ctttgtggtt aaaaaacgac gttgcataaa tcttcaaaaa atccaataaa
       61 atgataaaac aaatacttgc ccttgccaat gacctttccg ggttgttttt cccgaagctg
      121 tgcctggcct gtggcgaaca tttgctgaga ggcgaagatg ccatctgctc catctgccat
      181 tttcaccttc ccaaaaccta ttttcacaac gatcccgata atccgctgaa caaagtgttc
      241 tgggggcggg tgaacctgga gggggtggcg tcgtatctct atttccagaa ggggagcacg
      301 gtgcagcatt tgttgcatct gctgaaatat aaagggaaag aagagattgg cgtcaggctt
      361 ggccagtggt atggccttga tctgaaattc gccgctcctt tccgcgatgc cggcctgatc
      421 atcccggttc cgctgcatcc ggccaaaaag cggaaaagag gatacaacca gagcgaagca
      481 tttgccgaag ggctggcatc ggtgatgacg ggccggcttg agacgcgggc cctgtaccgc
      541 agggtcaaca caaagacgca gacccgcaaa tcgcgctacg accgctggga gaacgtagag
      601 aacatctttg ccgtcaggaa cccggaaaag cttcgctacc agcatgtttt gcttgtggac
      661 gacgtgctga ccaccggcgc caccctcgaa gcctgcgcac gggctttgct cgacgtgccg
      721 ggcgttaagg tcagcgtggt cacgattgcc tttgccagct attgatcacc gtccgcgggc
      781 cagcacacac gctttcttgt accggttcat cttcccgtcc attccaagga cttccgcaag
      841 gatcacgtaa atcccgtcgg gagccggcgt ccggtcatcg agtgttccat cccaggagta
      901 aaaggcagat gatccgggca gctcattgtc ggccagcttc cgggcaaagt acccgtttgc
      961 attaaaaata atgatcgtca ggagtttacc gggagaagta aagtcgaaaa aaatgcccag
     1021 gttgtcgtct ttgccgtcgc catcagggga aaagaccatg ggctccaccg aaatggcgct
     1081 tgtttcttcc ggaacggccg cgaggtattg cgaattgcga tagcccgggg tgccgaaccc
     1141 cgatgtttcc gcggcggagt gccagttggt ggcgtcgtca gcggagcgct ccgggctgat
     1201 ccgctccagc gaaaccccat cgtacgacaa cagcatcagg aaatgcatct cttccgaata
     1261 cctcatcccg tcgatctttt gatcgttatc atccattaga agaacatatc cttcatcatt
     1321 attgaaggaa gggaaagagc tcatttccag gaatgcttcc ggggctggtg tttcatactg
     1381 gttttgcacc attccgggat cggagcagag cgcgaggtac tctcccggga gcatcacccg
     1441 gcagccggca atgatcgtca atgcctgcgt atcggcaatc ccggccggat ctgtgctgat
     1501 ggaagccagc agccagttct ccaggttgat agccttttcc gaccggttat aaagttcgac
     1561 ataatcgacg ccgtcgtctt tcggattgaa aagaacctca ttgatgacga tttcgccggg
     1621 ttcggccaat tccggcagcg caaaagcctt ttccgtaaac tcttccgaaa ggataccggt
     1681 acaattggcc attcccggtt tcaccgacag ggtaaacgac tgccgggaaa taaatggcac
     1741 ggcaaaagca aggagggcgg tggtatgatc cggtgtggag agcgccaccg aaacaggagc
     1801 gccggtgtaa ggcgtgacct cataaaaggc cggatcggta gctgaaaggc tgtcgagcga
     1861 ttcgctgaat gtcacggaca acagcgtatc gctgagcagg cagcaggaaa ggaccgtgcg
     1921 gggaaggggt tcggtggcgt aagcagaatt gggtttcccg ggcgtacctc cctccatggc
     1981 gtacgaggcg gtccagtttt cccggccggc gcagggatta ccggtgtcga ccatctccag
     2041 cgcccatccg ccgtcatctt tcgcatcatc tccataccac gaaaggtcgt attggagata
     2101 gcagaccgtt aagccggaag aatccctgag atacagctcc gccccggagt tcgggaggtt
     2161 gaacgaaggg atcgcgatgg ccggggcaag gtagtggagg gtgccgtaag cgtcgtcgct
     2221 gcagagaatg acgaaatgac ccggattcag tattacggat ggcaggtcat gaagcgatgt
     2281 ggaaatcccc agtgaaaggc cttcgaggtc aatggcaaag ccgctccggt tgtgcaactc
     2341 aaggtattca aactccggca ggtcgagggg cggggaggga tcggccatga tctcggtgat
     2401 gatgatgtcg tatggctgtg ttttatagta caaaaccgga agaatgacca gtggcgcctc
     2461 attcccggca acatcgcgga ttccggtgat agtcagcgaa caggcggttc cgcttgacag
     2521 gttgttcgca aggttcagca gcacctgcga cggatcggcc aggcacagcg cttccgccgg
     2581 atgaccgatc ccctcatcga aaaggtagtt caacggattt ccggccgcag ttgcatccag
     2641 cggttcgctg aactccagga ggacgatgga agggttcagc gcctttgctt ttagcaatac
     2701 aggcggaata gagtcgatga tcaacgggcc ggcatagatg tcgtcgaaat agaatttcgt
     2761 aatattggag gaagtgtact ggcaatagat ggaataataa tttccttccg ggaaaacggt
     2821 atcggcgcac tccccggccg gttgcagcga cattcccccg gcggtgttgc agaaaaattt
     2881 ccagttacct tccgaatccc gggtgatgcg caaccgtagc gtattggtcg aattgccggt
     2941 atacccctgg tcaagcctca acagttctgt aaaaacagcg gaatcctggc gccagaagct
     3001 cacgctgtcg ctggttccgc cggcctggat gaaataaccg ttcaggggtt gctgcggatc
     3061 gggggaatcg gtcatgaggt agatccgtgc aaaattgctg ctgctggtat tgaagctcat
     3121 tttgatccag aactgccatt ccaggtcacc ggagaaaggc tcggccagcc ggagcgacga
     3181 aatgccggcc gcaggggcat tcaactgcag cgccggccgc tggttttccg gtactgccga
     3241 tgaatagctc agcgtaaaat gccccaggtc gccctgccag gccggaccgg tggtgaaatc
     3301 accgtcggag aaatcgtcgc gcacctgcgc agccaggcag acggggagca gaacaaagaa
     3361 aaggaggtgt ttcttcatgg tcttttctct gttaatccga tttttggcct atttataccc
     3421 ggtcacctcc aaattttttt ggaaattcac tatttttgca ccgttcaaac aaccccgtat
     3481 gaaaatttgt gtggtcggcg ccaccggcct tgtcggcagt gaaatggtaa aagtcctcgg
     3541 tgaatcgggc cctggccata tcgaatttta cccggtcgct tcggagcgtt ccgttggaaa
     3601 aatggtttca ttccggggca aggagcaccc ggtgatcggc atgcaggaag cggtcgaaaa
     3661 aagagccgat atcgcgcttt tctccgccgg ggcggccgct tcacgccagt gggcgccgct
     3721 gtttgccgct gccggtacca cggtgatcga caactcctcg gcctggcgcc aggatccgga
     3781 gatcccgctc atagtccccg aaatcaatgc ccatatcctc aattcatcgc acaagatcat
     3841 ctctaatcca aactgttcca cgattcagat ggtgatggtc cttgccccgc tgcaccggcg
     3901 gtttacgatc agaagagtag tggtttccac ctaccagtcg gtgacgggaa gcggcgcaaa
     3961 aggctacctg cagctcgaga atgaacggaa agggatcgac gggccgaaat tttacccgca
     4021 tacgatcgac ctgaacgtga tcccccacgg cggcgatttt accgataacg gctacacgac
     4081 cgaggaggtc aagctcgatt tcgagacccg gaaaattatc ggcgacgatt cgatcaaagt
     4141 gaccgcgaca gttgtcaggg tcccggttta tggcgggcat tccgaatcgg tcaacgtgga
     4201 attcgaacag ccatttgaaa cggaagaagt gctgaggata ctggcgaatg agaaagggat
     4261 tgccgtaatc gaccatcccg cgaaagcgga atacccgatg cccattacgg caaggggaaa
     4321 agacgaagta tttgtgggga ggatcaggcg cgatcttaca gtagctaacg ggctgaatat
     4381 gtggattgta tcggataacc tgcgaaaagg ggcggcgacc aatgcggtgc aaatagcaga
     4441 gtatttgctc gaaaataaac tggttggact tgatatgtag tcgtaaagta gtcataaagt
     4501 agtcgtaaag ttgtcataaa gtagtcgtaa agtagtcgta tagttgtcgt aaagctttga
     4561 agatatacca cagcatagat ctttttcagc cggtacccaa cccggttgtg acggtgggga
     4621 cctttgacgg agtccatcgc gggcatcagg ccatttttga cgccatgcgc aatgaggcac
     4681 tccggtgcgg aggtgaaact gtggttgtaa cattttatcc ccatccccgc ctggtgatcc
     4741 atcccgacag caagagcctc cgctttatca atacccagga acggaaatac gagatcatca
     4801 gccggagccg gatcgatcat ctgatcatca tcccttttac cagagagttt tccaatctct
     4861 catcgggcga atttgtgcgc cgctacctgg tcgagaagat taaaattcac cagctgatcg
     4921 tcggctatga ccatcatttc ggaaaagacc ggctgggagg gtacaatgag ctgaagggtc
     4981 tgggaaaaat tcacgggttt gaggtcagcg aggtgccggc catgatcatc gacgggatac
     5041 ctgtcagctc taccaagatc agaaatgctt taattgaggg aaatatccgg ttggccaatt
     5101 ccctgctggg gtacaattac tcgatctccg gcaaagtggt ttacggcaac cggattgggc
     5161 gcaccatcgg ctttccgacc gcgaatatcg aacaggaaga cgagtataag ctaatctcgg
     5221 ctgtaggggt ttatgcctgc ctggttgatc accacggcag gacgtacaaa gggatgggaa
     5281 atatcggcta tcgcccgact atcgatgtgg ggagcctgac cattgaggtg aatatttttg
     5341 attttgatga agagatctac ggcgaccgga tcatcattta ttttatcgaa aggataagag
     5401 atgaggtcaa gtttgaaaac cttacggccc tgagagacca gcttgtggcc gaccgggcga
     5461 aagtgctgga aatactcaga taattccagg ttccaggttt caggtttcag gttccaggtt
     5521 tcaggtttca ggtttcaggt tttacatcag tattcctaat tcactattca ctgttcact
//
LOCUS       DAOPNH010000235         5561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_16938_length_5561_cov_4.700872, whole genome shotgun sequence.
ACCESSION   DAOPNH010000235 DAOPNH010000000
VERSION     DAOPNH010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16938_length_5561_cov_4.700872"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..614)
                     /locus_tag="PKZ74_11440"
     CDS             complement(108..614)
                     /locus_tag="PKZ74_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93409.1"
                     /translation="MKKILFITIIIATSIFSAHSQDRPGISIFLHQTGMYYSSDNITS
                     EGLGLGIGAHFLHKSQIAGQVDANILWGNGNAITTRIAAGYEKKGKWAPAIYATCNLL
                     WGQRTEILAENGDRPPLPAWAAGVRVTPLKFRTGRGYVSALEFGYGVGPAKAMSLEFS
                     VLSIGIRF"
     gene            complement(611..1612)
                     /locus_tag="PKZ74_11445"
     CDS             complement(611..1612)
                     /locus_tag="PKZ74_11445"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM93410.1"
                     /translation="MNRLLNLTIVTFLALLVACGEKEGDRSGFEEATVVAQQVNGENL
                     MNSVAELVDIHLNDIPIDNSECPPGDLFPTDHLTRNAAVGYISEEFRKMGYIPDTAVL
                     GEGAYITYNVFAEWPGTSDAEEVILVGSHLDAFYGGADDNTSAVAAMLEAARIARNFS
                     FSHTIRFIAFDLEELGSIGSTRYVEGGLAGDVKTAIVMDLIGYRSFEPGSQKSVMGIR
                     LPDVGDYLMVIGNRDSEEITRQITDMANSQSLSKALGVLAPGDGAYFLSQALMRSDHG
                     LMWYKGIPAVFFTDGANFRNPNYHLASDLPETIDADFLTGNTKVLMAAIALLAEVQL"
     gene            complement(1636..2178)
                     /locus_tag="PKZ74_11450"
     CDS             complement(1636..2178)
                     /locus_tag="PKZ74_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93411.1"
                     /translation="MKKIISSLVALLFFLPLFAQEKQDKKSFEVTAGYSMLFYQSAYN
                     LQNASGIEASIGREISDPLRWQAGLRVGLDPLLPEVFGRILLQEEVGSWRPQIGLELG
                     VTGRAKFEDGAFLLRETREAMQTGIDAGYLAIHTAPLSFRISKNWNLSIAEIDFGTHF
                     TRIGRTLRAQVTLISAGMKF"
     gene            complement(2250..2882)
                     /locus_tag="PKZ74_11455"
     CDS             complement(2250..2882)
                     /locus_tag="PKZ74_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM93412.1"
                     /translation="MDKNVLERKTLFVVLLTAVTMIVEIFFGLTTKSMALLADGIHMG
                     SHVLAIGLSWIAYVFIRRSKASGRFKGNPDKVLSLSGYSSGLMLLIFAFFILSEAIKR
                     SVNPLPIDYKEAIIVACIGLIVNIISAFILHHDKSQSDHNIRAAYLHVLADALTSVSA
                     ILGLTAALIWNIPIIDAMAAILSSLVIIRWSVGLILDSGKSLLDIHNQKK"
     gene            complement(2954..4366)
                     /locus_tag="PKZ74_11460"
     CDS             complement(2954..4366)
                     /locus_tag="PKZ74_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM93413.1"
                     /translation="MKILLIAPCQVDQQRPNSMDIPQLTLSLIAALTPPDHEVEIVEE
                     VYGETVNFDGDYDLIGISIMTQTAIRGYQVANEFRKRGKTVVFGGIHATSMPDEAIRY
                     GNAVVIGEAEGLWDIVLEDAKHNRLKSFYKLDKLPDLQKHIRPRRDLIKCSSGRFAIA
                     PIETTRGCPYNCDFCSVSRFFGTRQRHKPIPDIIADIESCREKNLFFLDDNITGDKKY
                     ARELFREMIPLNKIWVGQASVQVANDPELMNLAYRSGCRALLIGFESMSDSGISQYRK
                     TLKTIDENVSAVKKLQDNGIMTMASLIFGLDTDTPDVFDVAHDFLTRSKAAFFQACVM
                     TPYPGTPVFNKLRNEGRILTDDWTRFDASKVIIRPENLSPEELLEGYNKMKYHFYSNK
                     SILQRSYPNIKIGFYEALLYFTLNKGYQKKNNPSVFSQVYENPSGHPVDFNVAKYVRP
                     LINGHAPVFSEKLSEVNRIR"
     gene            4691..>5561
                     /locus_tag="PKZ74_11465"
     CDS             4691..>5561
                     /locus_tag="PKZ74_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HPM93414.1"
                     /translation="MKKKIKFCLVYRDMWQSSGKYQPHVGQLKAVAPLIIEMECFSRV
                     ETNGGAFEQVQLMYGENPNVAVREWTKPFNEAGIQTQMLERALNGIRMYPVPKDVRRL
                     MFTVKKAQGVDIARSFCGLNDHRNLELSIKYAKEAGMISQAALSITFSPVHTVNYYMD
                     VVKKMVKYGAEEICLKDMAGIGRPVMLGELVKKIKKKHSHIKVQYHGHSGPGLSMASI
                     LEVARAGADYIDVAMEPLSWGMVHPDILSVHALLRDAGFKVPDINMNAYMKVRSMTQE
                     FIDDSLGLFIDPRN"
BASE COUNT         1457 a         1333 c         1335 g         1436 t
ORIGIN      
        1 tttcccaggt tgacgatgac gtcaatatcc cgattgagcg gatcgttgat attgtcgaca
       61 ggatagtggc agcatcgcat tgaaaaaggg ttgctgcccc ggtaagatca aaatcttatc
      121 ccgatggaaa gcacagagaa ttccaggctc atcgcctttg caggtccaac gccataacca
      181 aattccaaag ctgagacata tcctcttccg gttctgaatt ttaacggtgt taccctgacg
      241 cctgccgccc aggctggtaa aggaggtctg tctccatttt ctgcaagtat ctctgttcgc
      301 tgaccccata acaggttgca ggtcgcatag atcgccggtg cccacttccc ctttttctca
      361 taacctgcag caattctggt tgttatcgca ttcccgttgc cccacagaat attggcgtca
      421 acctggccgg caatctggga tttatggaga aaatgggccc cgattcccag cccgagacct
      481 tcgctggtga tattatctga agagtaatac atcccggtct ggtgaaggaa aatgctgatc
      541 ccgggtctgt cctgagaatg ggcagaaaat atgcttgttg cgataataat ggtaatgaat
      601 aaaatctttt tcatagctgt acctccgcta gtaaggcaat ggctgccatc agaacttttg
      661 tgttaccggt cagaaaatct gcatcaatgg tttcaggaag gtcggaagcc agatggtagt
      721 taggattcct gaaattggct ccatctgtaa agaagacggc agggattcct ttgtaccaca
      781 tcaatccgtg gtcactccgc atcagggcct gcgacaggaa ataagctcca tcgcccgggg
      841 ccaggactcc caatgccttt gacaaacttt ggctattcgc catatccgtg atttgcctgg
      901 taatttcttc agaatccctg ttgccgataa ccatcagata atcacccaca tcaggcagac
      961 ggattcccat aacactcttc tggcttcccg gttcaaaaga acgataaccg atcaaatcca
     1021 tgacaatggc tgttttcaca tcgccggcca gtccaccttc aacataccga gtactgccaa
     1081 tcgaacccag ttcttcgagg tcaaatgcta tgaaccggat cgtgtggctg aaactgaaat
     1141 tccgggcaat tctcgcagct tcgagcatcg cagcgaccgc tgaagtgttg tcatcagccc
     1201 ctccgtagaa ggcatcaaga tggctcccca ccaggataac ttcctccgca tctgatgtcc
     1261 ccggccattc ggcaaaaaca ttgtaggtga tgtatgcacc ttcgcccagc acggcggtat
     1321 cgggaatata acccatcttc cggaattctt cgctgatata acccacagcg gcattccgcg
     1381 tcaggtgatc ggtcgggaac aagtcgccgg gcgggcattc gctgttgtcg atcgggatgt
     1441 cgttcagatg tatatcgacg agttctgcaa cgctgttcat caggttttct ccgttcacct
     1501 gttgtgccac aaccgtagcc tcttcaaatc ctgatctgtc accctctttt tcaccgcagg
     1561 ccactaacag tgccaggaaa gtgacgatgg ttaagttcaa taatctattc attttcatca
     1621 caataaaatt aatccctaga atttcattcc ggctgagatt aaggtcacct gagcgcgcag
     1681 ggttcggcca atccgtgtaa aatgggtgcc gaaatcaatt tccgcaatgc tgagattcca
     1741 gtttttactg atcctgaacg acaggggagc ggtgtggatg gccaggtagc ctgcatcgat
     1801 accggtctgc atagcttccc gggtttcccg aagaagaaag gccccatctt caaactttgc
     1861 cctgcctgta actcccagtt ccaaaccgat ttgcggacgc caggaaccga cctcctcctg
     1921 aagcaagatc cggccaaaaa cttcgggcag taaaggatct aatccaactc tgagcccggc
     1981 ctgccatctt aaaggatcgc tgatttctct cccgatggaa gcttcgatac cggaagcatt
     2041 ctgcagattg taagccgact ggtagaagag catgctatag ccggccgtaa cctcaaagct
     2101 ttttttatcc tgtttttcct gtgcgaacaa aggcaggaaa aaaagcaatg ctacgagtga
     2161 agaaattatc tttttcatat taacttttaa tgatccggga atgataacca atgccaatga
     2221 aattctcaac gggtgagggc attatgttgt tatttttttt ggttatggat atcgagtaaa
     2281 gattttcccg aatcaaggat cagccctacc gaccagcgga tgatcacaag tgagctgaga
     2341 atggccgcca tcgcatctat aatcgggatg ttccagataa gcgccgctgt cagacccagt
     2401 atagctgaaa cactcgtaag ggcatcagcc agcacatgca ggtaagcagc ccggatgttg
     2461 tgatccgact ggcttttgtc gtgatgcagg ataaatgcgc ttataatatt gacgatcaga
     2521 ccgatacatg ctacaatgat agcttccttg taatctatcg gaaggggatt gacgcttctt
     2581 tttatggctt cggaaaggat aaaaaaggcg aagatgagca acatcaggcc actgctataa
     2641 cctgagagcg acaggacctt atccggattg cctttgaatc tgccgctggc cttcgatcgc
     2701 ctgataaaaa cataggctat ccagctcagt ccgatggcca gcacatgcga tcccatgtgg
     2761 atgccgtcgg ccagtaaggc catcgatttt gtcgttaatc cgaaaaaaat ctcgacaatc
     2821 atcgtgacgg ccgttaaaag cacgacaaac agggttttcc tctccagtac attcttgtcc
     2881 atggcaccag caaatcagga ttgcgacgga aattagtgcc ggtaaaatta caaaaggatt
     2941 cgggatatta tccttaacgg attctattaa cctccgacaa tttttcactg aaaaccggag
     3001 catgcccgtt tatcaaaggc ctgacatact tcgccacatt gaaatccacc ggatgaccgg
     3061 atgggttttc atatacctgt gaaaacaccg acggattgtt ttttttctgg tatcctttat
     3121 ttaaagtgaa atagaggaga gcttcataga agccgatctt aatgttagga taagaccgtt
     3181 gaaggatgga tttgttcgaa tagaaatgat atttcatttt gttgtagccc tccaacaatt
     3241 cttccggtga aaggttttcc ggacggatga tgactttcga agcatcgaac cgggtccagt
     3301 cgtcggtgag aatccgtcct tcgtttcgta atttgttgaa taccggtgtc ccgggataag
     3361 gggtcattac gcaggcctgg aaaaatgcgg cttttgagcg ggtgaggaaa tcatgggcaa
     3421 catcaaacac atccggtgtg tcagtatcga ggccgaagat cagcgatgcc atggtcatga
     3481 tcccgttgtc ctgaagcttt ttcaccgcgc tgacgttttc atcaatagtt ttcagcgttt
     3541 tccggtactg gctgatcccc gaatccgaca tcgattcgaa cccgatgagc aaggccctgc
     3601 atcccgatct gtaagcgaga ttcataagct ccgggtcatt ggcgacctgt accgatgcct
     3661 ggccaaccca gatcttattc aacgggatca tctccctgaa cagttcccgg gcatattttt
     3721 tatccccggt aatgttgtcg tcgaggaaga acaggttctt ctcacggcat gattcaatgt
     3781 cagcgataat atcgggtatg ggtttatgcc gctggcgggt gccgaaaaac cgcgaaaccg
     3841 aacaaaagtc gcagttataa gggcaaccgc gggtggtctc aataggggcg atggcgaatc
     3901 ttcccgatga acatttgata agatccctgc ggggccttat atgtttttgt agatcaggaa
     3961 gtttgtcgag tttgtagaac gatttcagcc ggttgtgttt agcatcttcc aggacgatat
     4021 cccacagccc ttctgcctcg ccgataacga ctgcgttacc atacctaata gcctcatcag
     4081 gcatcgatgt agcatggatc cccccgaaaa ccactgtttt acctcgtttc cggaattcgt
     4141 tggccacctg gtacccgcgg atggccgtct gcgtcatgat cgaaatcccg ataaggtcgt
     4201 aatccccgtc gaaattgacc gtttctccat acacttcttc cacaatttcc acttcatggt
     4261 cagggggtgt cagggctgct atcaaagaaa gtgtcaactg gggaatgtcc atcgaattcg
     4321 gacgttgctg atcaacctga cagggtgcaa ttagtaatat tttcatcgtg ataattatgc
     4381 tttgcttaaa agctgtcgcc tcatttatcg cagagcatag gaaatattac cggatcaaat
     4441 gcagtatgac tgtaatgtcg atatcctctg taactttaag gtaatgtaac gaatataata
     4501 acgggctaaa agtaaataaa ttataaaagc ggtgatttag aataatggat atacgaattt
     4561 ttttaaaaga cgaaaataaa tacgcctgcc ggcgttgata tgatttaatc ctgataattt
     4621 tgcggaacct gtgaaaatgc gattttccgc aaatgactat taaattacgg accaatagat
     4681 ttataaccga atgaagaaaa agataaagtt ttgcctggtt taccgcgaca tgtggcaaag
     4741 ctcgggaaag taccagcccc atgtcgggca gcttaaggcg gtagcccctt taatcattga
     4801 aatggaatgt ttctcgcggg tggaaaccaa cggaggcgcc ttcgagcagg tacagttgat
     4861 gtatggagaa aatccaaacg tcgccgtcag ggaatggacc aaacctttca atgaagcggg
     4921 tattcagacg cagatgctgg aaagggcttt gaatggtatc aggatgtatc ccgttccgaa
     4981 ggatgtaagg cgattgatgt tcacagtgaa gaaagcacag ggagtcgata ttgcccggtc
     5041 gttttgcgga ttgaacgatc accggaacct tgaactatcc atcaaatacg cgaaggaggc
     5101 ggggatgatc tcgcaggccg ccctctccat cactttttcc ccggttcata cggttaatta
     5161 ctacatggat gtggtgaaaa agatggttaa atacggcgct gaagagatct gcctgaagga
     5221 tatggccggg atcggacggc cagtgatgct gggcgagctg gtcaaaaaga tcaaaaagaa
     5281 gcactcccat atcaaggttc aataccacgg acattcaggc ccggggcttt cgatggcttc
     5341 gatcctggag gtggccaggg ccggtgccga ttatatcgat gtggcgatgg aaccgctgag
     5401 ctgggggatg gtccatcccg atatcctctc cgttcatgcc ctcttgcgcg acgcaggctt
     5461 caaagtgcct gatatcaaca tgaatgctta tatgaaagtc aggtcgatga cgcaggaatt
     5521 tatcgatgat tccctcgggc tgtttatcga tccacggaat a
//
LOCUS       DAOPNH010000236         5535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17027_length_5535_cov_5.931569, whole genome shotgun sequence.
ACCESSION   DAOPNH010000236 DAOPNH010000000
VERSION     DAOPNH010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17027_length_5535_cov_5.931569"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="trmB"
                     /locus_tag="PKZ74_11470"
     CDS             complement(<1..523)
                     /gene="trmB"
                     /locus_tag="PKZ74_11470"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPM93415.1"
                     /translation="MKKKLAHFAENLTFSHLFQYTQLDLLQEFPLKGKWHSGFFMNEN
                     PVTLELGCGKGEYTVNLAMLHPERNFIGVDIKGARLWKGSKMTQDMGLKNVAFIRTRI
                     EHITYFFSHNEVDEIWLTFPDPQPQSNRINRRLTSPVFLERYRQFLTPGAVIHLKTDN
                     TMLYRYTLMVIREN"
     gene            complement(546..1568)
                     /locus_tag="PKZ74_11475"
     CDS             complement(546..1568)
                     /locus_tag="PKZ74_11475"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93416.1"
                     /translation="MKPVDHHLRQLLYEYDCVTIPGLGGFIMQSVPSRLNQTRQRIFP
                     PNRYPAFNSFLNHDDGLLISHIARHNNLSYSQASLFISEFVSECQIRFTLGQRVVLEG
                     IGEMFHGDDGTVQFIQKDTLNFNPGSTGMDSLALIPVSGESKTIRKQPKPEDRKTPAA
                     RKEKQPASARWTVAVSLPIILFLIYGIIFPASVQNLYTEFAGILSGLREESFYKTSDK
                     KPVSAPLTVNEPVRAKTSEPVPLPPETETEVKIDPVIQGAETGAIITGPRYYIIGGCF
                     EHDKNAERFLVTLISKGFDAELAGKTNRGHTRVSYKSFEEKEPALSYLIFIRKEENAS
                     AWLLKY"
     gene            complement(1623..2372)
                     /gene="kdsB"
                     /locus_tag="PKZ74_11480"
     CDS             complement(1623..2372)
                     /gene="kdsB"
                     /locus_tag="PKZ74_11480"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPM93417.1"
                     /translation="MNCLAIIPARYGSSRFPGKPLAMINGRSMILRVCDRVKSAGVFI
                     DQVVATDDERILTHVLDNGYQAIMTSEDHQSGTDRCLEALETWESSPASPVEFVVNIQ
                     GDEPFIQPEQIRLLYDTLISPGTGIATLAKLIDQKEDIFNPNVVKVVFSSEYKALIFT
                     RSPLPYVRRSGEMEWIHEQAHYKHIGIYGFRSEVLRQICRLPQGRLEKLESLEQLRWL
                     ENGFDIKLAITVLESVAIDTPADLLKITNTA"
     gene            complement(2379..2993)
                     /locus_tag="PKZ74_11485"
     CDS             complement(2379..2993)
                     /locus_tag="PKZ74_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPM93418.1"
                     /translation="MHIAIAGNIGAGKTTLTELLSRHFGWEPHFEDVEANPYLNSFYE
                     DMQRWSFNLQIFFLASRFRQILNIHASGKNVIQDRTIYEDAYIFAPNLHAMSLMETRD
                     FENYSSIFELISSLIKPPDLLIYLRSTVPNLVKQIQKRGRTYEESIRLDYLKSLNERY
                     ETWISRYKQGKLLIINVDEINFADNPHDLGVVIEKIQAEIHGLF"
     gene            complement(2998..4506)
                     /locus_tag="PKZ74_11490"
     CDS             complement(2998..4506)
                     /locus_tag="PKZ74_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPM93419.1"
                     /translation="MALINSVLYWLIKKRIHQIDLFIKYPHDVQAEWFRRLITTAKDT
                     EWGKEYGYSSISNVGQYRERVPVSTYEDLKGRIDLMRQGKQNILWPTDIRWFAKSSGT
                     TDDRSKFIPVSQESLEECHYKGGKDMLSLYCNNFPNTALFSGRGLAIGGSHKISEINA
                     ESYYDGDLSAIIIQNLPFWAEFIRVPRKSTALMDNWEEKIEMMADETIPHNLTSLSGV
                     PSWMLLLLRRVLEKTGKSDIMEVWPNLEVYFHGGVNFTPYREQFRQIIRAGEMNYMET
                     YNASEGFFGLQDCSDSSELLLMLDYGIFYEFIPVSELDNDHPRACNLDEVDTETHYAM
                     VISTNAGLWRYLIGDTVKFTSINPFRIRITGRTKNFINAVGEEVIIDNAEKALAVACR
                     KCHAVVNEYTAAPLFLEKEKKVAHEWLIEFDTPPPDLEYFTETLDNALKSLNSDYEAK
                     RFHDLILLPPVIRIMAPGTFYNWLKNKGKLGGQNKVPRLSNHRTIIEEILTV"
     gene            complement(4612..>5535)
                     /locus_tag="PKZ74_11495"
     CDS             complement(4612..>5535)
                     /locus_tag="PKZ74_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPM93420.1"
                     /translation="DYYLNFIPYFINLFSYLFTFISVIYFTSHMAGNSEIIAILSSGI
                     SYNRMLRPYFISAILLALLSFYLANFLIPHTNQKRRAFTDVYFGNLSKSKDEHIHLQI
                     EPGTYVYVESFNIKTKTGFRFSLEKYDGHDMKYKLMADRIIWDTLTSRWTIQNYTIRE
                     ISGNKEKISHGTRVDTTLNLMPSELYIKKENYEEMTFGELQDYIRKEKLRGAEEIVYY
                     DMERHNRLASPFATLVMTLIGVSLSSRKMRGGIGMHLGLGILLTFTYILLMRVTTVFA
                     TVGDLPPYLAAWIPNIFFGIIGLYLLKSAPK"
BASE COUNT         1335 a         1285 c         1349 g         1566 t
ORIGIN      
        1 tattttcgcg gatcaccatc aatgtataac ggtataacat ggtattgtcg gttttcaggt
       61 gtatgaccgc cccgggggtg agaaactgcc ggtatctttc caggaagacc ggcgatgtga
      121 gccgcctgtt tatcctgtta ctctgtggct gcggatcggg aaaggtcagc catatctcat
      181 ccacttcatt atgtgaaaag aaataggtga tatgttcaat cctcgtcctg ataaaagcca
      241 cattctttag ccccatgtcc tgggtcattt tgctgccttt ccaaagcctg gcacctttta
      301 tatccacgcc gatgaaattt ctttcaggat gtagcatggc caggttgaca gtatattctc
      361 ctttgccgca gcccagttcc agcgtaacag gattttcgtt catgaagaat ccgctgtgcc
      421 actttccttt caggggaaat tcctgtagca gatccagttg ggtatattga aacagatgac
      481 tgaatgtcag gttctcggca aaatgggcca gtttcttctt caccacttcg atcgggaaat
      541 ttgtatcagt atttcagaag ccaggcagaa gcgttttctt cctttcgaat gaaaatcaaa
      601 taagataagg caggctcttt ctcctcaaaa cttttatagc tgacccttgt gtggccgcgg
      661 ttggtctttc cggccaattc agcgtcaaat cccttgctga tcagtgtcac gaggaatctt
      721 tccgcgttct tgtcatgctc aaagcaaccc ccgatgatat agtacctggg tccggttata
      781 atagcaccgg tttctgctcc ctgtataaca gggtctattt tgacttctgt ttcagtttcc
      841 gggggaaggg gcacgggttc gctggttttt gcccggaccg gttcgtttac cgttagcggt
      901 gcggataccg gttttttatc cgatgttttg taaaaggatt cttctcttaa cccggataag
      961 atcccggcaa attcggtgta gagattttgc accgaagccg gaaagataat gccataaata
     1021 aggaaaagga taatcggcaa tgaaacggcc acagtccacc tggctgatgc cggctgtttt
     1081 tctttgcggg cagccggggt tttacggtct tcaggctttg gttgtttccg tatggttttg
     1141 gattcgccgg aaaccgggat cagggccagg gaatccattc cggtggatcc gggattaaaa
     1201 ttaagggtat ctttctggat gaattgcacc gtgccgtcat ctccatggaa catttccccg
     1261 attccttcca gcacgactct ttgcccgagg gtaaacctga tctggcattc gcttacaaat
     1321 tcactgataa aaaggcttgc ctggctatac gacaggttgt tatgacgggc aatatggctg
     1381 atcagaagcc cgtcgtcgtg attgagaaag ctgttgaagg ccggataacg gtttggcggg
     1441 aagatccgct gccgggtctg gttcagccgt gacgggaccg attgcatgat aaatccaccc
     1501 agtcctggga tggtcacgca atcatactca tacaataatt gccgcagatg atgatcaacc
     1561 ggtttcattc aaaagttttg ctggattttc ctttgcgaaa ataggaataa gtgccctaaa
     1621 ggtcaagccg tgttagtaat ttttaataaa tccgcaggag tatcaatggc aacgctttct
     1681 aaaaccgtga ttgccaattt tatatcgaat ccgttttcca gccatcgcaa ttgttccagg
     1741 gattcgagct tttcaagtct cccctgagga agccggcaga tctgccggag aacttctgac
     1801 cggaaaccgt agataccgat atgcttataa tgcgcctgtt cgtgtatcca ttccatttcg
     1861 ccggatcttc tcacataggg caagggagac ctggtgaata tcaatgcttt gtattcactg
     1921 ctgaaaacaa ctttcacaac gttgggattg aatatatctt ccttctggtc gattaattta
     1981 gccagggtag ctataccggt tcccggggag atgagtgtat cgtaaagcag cctgatctgt
     2041 tccggctgta taaagggttc atcgccttgt atattgacga caaactcgac cggcgacgcc
     2101 ggtgaggatt cccaggtttc aagtgcttcg aggcatctgt cggtgccgct ctggtgatct
     2161 tctgacgtca taatggcttg ataaccatta tccaggacat gggtaagaat ccgttcatca
     2221 tctgtcgcca cgacctggtc gatgaaaacg cctgccgatt tcacccggtc gcagacgcgg
     2281 aggatcatgg agcggccatt tatcatcgcc aggggttttc cagggaaccg cgatgacccg
     2341 taccgggccg gaatgattgc cagacagttc atagattatc agaaaagccc gtggatttca
     2401 gcctgtatct tctcgatcac cacgcccagg tcgtgcgggt tgtcggcaaa gtttatctca
     2461 tcaacattga ttattaggag tttgccttgt ttataacggg agatccaggt ttcataccgt
     2521 tcgttaaggc tcttcaggta gtccaaacgg atcgattctt cataggtcct gcctcttttc
     2581 tggatctgtt tgaccaggtt cgggaccgtc gaccggagat agatcaacag gtcgggaggt
     2641 ttgatgagcg aactgatcag ctcgaagatg gagctgtaat tctcgaaatc ccttgtttcc
     2701 atcaggctca tggcatggag gttaggagcg aagatatagg catcttcgta aatggtcctg
     2761 tcctggatga catttttccc actggcatga atattcagga tctgccggaa gcggctggcc
     2821 aggaagaaga tttgcaggtt gaacgaccag cgctgcatat cttcataaaa actgttgaga
     2881 taaggattcg cttctacgtc ttcaaagtgc ggttcccatc cgaaatgcct tgaaagaagc
     2941 tcagtgagtg ttgttttgcc ggcgcctatg ttgcctgcga tagctatatg catagtgtta
     3001 tacggttaat atttcttcaa taattgtacg atggttcgat aatcggggaa ctttattctg
     3061 tccacccagc ttgcctttgt ttttcagcca gttgtaaaaa gttcccggtg ccatgatccg
     3121 gatgaccggg ggcagtaaaa tgagatcatg aaaccgtttg gcttcataat ccgagttaag
     3181 cgatttaagg gcattatcca atgtttccgt gaaatattcc aggtcgggtg gaggagtgtc
     3241 aaattctatc agccactcat gagccacctt tttctctttc tcaaggaata acggcgcagc
     3301 agtatattca tttacaacag cgtgacattt tctacaggct acggccagcg ctttttccgc
     3361 attgtcgatg atcacttcct caccaactgc gttgatgaag ttttttgttc tgccggtaat
     3421 cctgatcctg aaaggattaa tggatgtgaa tttgacagta tcgccgatca ggtaacgcca
     3481 caggccggcg ttggtggaaa tgaccattgc gtaatgggtt tcggtatcca cttcatcaag
     3541 gttgcaggcc ctgggatgat cgttgtccag ttcactgacg gggataaact cgtagaaaat
     3601 gccgtaatcc agcataagca gcagttcgct ggagtcggag cagtcctgca gaccgaagaa
     3661 accttcagag gcattgtagg tctccatgta attcatctcc ccggcccgga tgatctgtct
     3721 gaattgttcc cggtaaggcg tgaaattgac cccgccatga aaatatactt ccaggttcgg
     3781 ccagacttcc atgatatcgc tcttgcccgt tttttcgagg acccttcgca aaagcaggag
     3841 catccacgac ggaacgccgg aaaggctcgt caggttgtgc ggaatggttt catcggccat
     3901 catctctatc ttttcctccc agttatccat cagggctgtg ctttttctgg gaacccggat
     3961 gaattcggcc cagaatggca gattctggat gatgatggcc gaaaggtcgc cgtcatagta
     4021 cgactcagcg tttatctcac tgatcttatg gcttccaccg atggccaggc cacgcccgct
     4081 gaacaaagcc gtattgggaa aattgttgca atagagcgaa agcatgtctt tgcccccttt
     4141 ataatggcac tcctccagcg attcctggct gaccggaatg aatttgctcc ggtcgtccgt
     4201 cgtgcccgac gatttggcaa accaacggat gtcggttggc cacagaatat tctgttttcc
     4261 ctgtcgcatc aggtcaatcc ggcctttcag gtcttcgtag gtgctgacag gtaccctctc
     4321 cctgtactgg ccgacatttg aaatggacga atagccgtat tctttgcccc attcggtatc
     4381 ctttgccgtt gtgatcagcc tgcggaacca ttcggcctgc acatcatgcg ggtatttgat
     4441 gaacaggtcg atctggtgga tccgcttctt gatcagccag taaagtacgg aattgattag
     4501 cgccatttcg gatttctgct tgtgcgattc atcttcaaaa ataacatttt cgtaaacaaa
     4561 gggagcgatt gttcaaactt tttgctttag cgccggagat gtaagaccgg cttatttagg
     4621 cgccgatttc agcaggtaaa ggccaatgat cccaaagaaa atatttggaa tccaggcggc
     4681 caggtagggg gggaggtcac ctactgttgc aaaaacagta gtcaccctca ttagtaaaat
     4741 ataggtaaat gtcagtaaga tcccaagtcc cagatgcatg ccgatccctc ctctcatttt
     4801 cctgctggat aatgaaacac cgattagcgt catcaccagg gtggcgaacg gagatgctaa
     4861 acggttgtgc ctttccatat cgtaataaac gatctcttca gctcccctga gcttttcctt
     4921 ccttatataa tcctgtagct ctccgaaggt catctcttca taattctcct ttttaatata
     4981 aagctccgaa ggcatcaggt tcagtgtggt gtcaacgcgg gttccatggc tgattttttc
     5041 tttgtttccc gaaatctccc ggatggtgta gttctgaatg gtccaccggc tggtcagcgt
     5101 gtcccagata atgcggtcag ccatcaattt atatttcata tcatgaccgt catacttttc
     5161 cagagaaaaa cgaaatccgg tttttgtttt aatattgaaa ctttcgacat aaacataggt
     5221 acccggttcg atctgcaaat gaatgtgttc atctttgctt ttcgacaggt tcccgaaata
     5281 gacatcggtg aaagcccttc tcttctggtt tgtgtggggg atcaggaaat tagccaggta
     5341 aaaggagagc aatgccagga ggatagccga gataaaatag ggcctcagca tccggttgta
     5401 actgataccg ctgctcagaa tggcaatgat ttctgaattt ccggccatgt gggatgtgaa
     5461 atagatgacc gatatgaatg tgaaaagata gctgaacagg ttaataaaat aagggataaa
     5521 attcagataa taatc
//
LOCUS       DAOPNH010000237         5532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17043_length_5532_cov_6.340515, whole genome shotgun sequence.
ACCESSION   DAOPNH010000237 DAOPNH010000000
VERSION     DAOPNH010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17043_length_5532_cov_6.340515"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKZ74_11500"
     CDS             complement(<1..224)
                     /locus_tag="PKZ74_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93421.1"
                     /translation="MNTILFLIILFLSFWVFILPKSYKYYYTLVLLAGGLALSTIWSF
                     RVFDGSDEQLIVRLDFQTFHSSIVFTIDRLS"
     gene            complement(269..4684)
                     /locus_tag="PKZ74_11505"
     CDS             complement(269..4684)
                     /locus_tag="PKZ74_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93422.1"
                     /translation="MRIKLRLILVAAITVLFNNLQAQVEKPEETYILRPLSAVDEVQL
                     MSLPELKPPAGFENRDLPYMVDNSTQMYMRPAYQQAGLSCGQASCIGYNFTYEMARER
                     NLNASLLQNQYPTHFAWNFMNGGEGWYGVSYLHSIQILKEYGMMNAVDYGGSLSYGGA
                     SRFLSGYNEYYNGMHNRITNAYQIQVGTPEGLLVLKHWLHNHLEGSAVGGVASFYAQH
                     MAVTTMLPWGTPEAGKYVLTYFGGSANHAMTIVGYNDSIRWDYNSDGLYTNNIDINGD
                     GTVNMKDWEIGGFKMVQSYGGVPNWGDQGFAYMMYKTVADNLGSGGIWNHCVHVLDVK
                     GALDPKLTAKIILKHDRRERIKVLAGIANNVGAQRPEHQLGFPIYDYQGGNRYMQGGT
                     TEADKTIEIGLDLSKLLSYSDIGDPVKIYLQVVENDPDNANTGEVIHFSIFDHTSGGS
                     QVVCAQTNVPLVNDDTTTLYLNHTFNFDRVHIVDETIPPAPEGEYYSHQFTVAGGTPP
                     YIWEFDKSYDESGQPASFPQITGTQLTPGNSSSGYVTQAIPFSFPFYDSAYSSITLHV
                     DGYLMFDQQLYPFPYYQDDKVLFNITRHISPFLNYHQEINAGQGCGIWYEGSADYVTF
                     RWKTALTENPAMTFNYAARLYPDGTIKFYYGQMNGCDMYLWMAGIGDGDGFNFQETRL
                     SNKPSVNANTSTTLVPYDYPEEMTLSETGLFSGTPEQSYGNEQITFKVTDNNFIQTKK
                     TFTFTSTGIIISDSVISGGDPVIAFSETANMSISVLNLKPETIPDAVMTITIDDPYIT
                     VTDNTENLGDLAFNLPRNVLNAFQFTVASDIPNERLIIVETSITSDTNVWISELYHYA
                     YAPVVTAENVEVNDENGRLDAGETSDVVVSYLNTGGVELTSVNTLLTTADPLVMINQS
                     VGNIPLLEVGILANLSYNVFVSPDCLPGHEIEFALNISGDGNYATTDTFSLVVGLYSE
                     NFESGDMELFTWGHEGDGQWRTDTYAPYEGTFAARSGNITHNQESVMVIDMDYLTDGV
                     ISFYQRTSCEGDTTSADNYDYLSFKIDGNEMERWDGETGWSYAEFPVTEGFHRFEWIY
                     HKDHNTNYRMDAAWVDLISFASSVNCSPGLQFSPSSVDFLLRTGETETKSLALSNPAS
                     GPLNFTADIAGIQPSVVKDGKGNRNIEGSYLVTDHDRFHAGQEYVWNFRTYNAGNDNE
                     WIKQIYITFPQGIMLVAATDFTGGSGGPMVFQGPFGNGVTAHWFGEDANGWGVVHMGE
                     LAASDVSVVPADNLLTDVQIYYEVQGEVYGSPPHTVTGNITLRNLGPVVPWLSIDNIE
                     GLLEGESVDSLRVTVDANGMDDGEYHAWLYLEDNFNGAFVIPVNMTIDTHMGELETGG
                     KRQEGIFNAYPNPFIAQTVLSILLPETTKTRIEISTLQGQIMKVIDQEVKGGIRHLIE
                     WDGNNEEGKPVGAGIYSGRLITGNKSYFVKIIKIR"
     gene            5068..>5532
                     /locus_tag="PKZ74_11510"
     CDS             5068..>5532
                     /locus_tag="PKZ74_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPM93423.1"
                     /translation="MFEKIKEAAGYLRSKGMVAPEVGIILGTGLGKLADHIDVEITID
                     YEDIPHFPISTVEFHHGVLIYGNLRGRKVLVMKGRSHAYEGYTTQQITFPIRVMKMLG
                     ISSLLVSNACGSMNPAFKKGSLMLIEDHINLLPNPLVGPNIDELGTRFPDMSQ"
BASE COUNT         1282 a         1375 c         1419 g         1456 t
ORIGIN      
        1 gaaagccgat cgatagtaaa gactatactg ctgtgaaaag tctggaaatc aagcctgaca
       61 ataagttgct catcgctccc gtcaaatacc ctgaaactcc atatggtgct cagggccagt
      121 cctcctgcca gtaaaaccag ggtgtaatag tatttataag atttaggcag aatgaatacc
      181 cagaaggata agaataaaat tatcagaaat aatatcgtat tcatgattta actgatgtgt
      241 ttacctggcc attcgcagct gcactttatt atcttatttt gattatttta acaaaatagg
      301 atttattacc ggtaatcagc cgcccgctat aaatacctgc gccgactggc ttgccctctt
      361 cattattgcc gtcccattct attaaatgcc tgatgccacc tttaacttct tgatctatca
      421 ctttcatgat ctgtccctgc agagtcgata tttcaatccg ggtttttgtg gtttccggca
      481 aaagaatact taaaacagtt tgtgcaatga atggattggg atatgcattg aatattcctt
      541 cttgccgctt gccgccggtt tcaagttctc ccatatgcgt atcgatcgtc atattcaccg
      601 ggatgacaaa cgcaccgttg aaattatctt cgaggtaaag ccaggcatgg tactcgccgt
      661 catccatccc attggcatcc accgttaccc gtagcgaatc aacactttct ccttccagca
      721 acccttcgat attgtcgatg ctcagccagg gtaccacagg gcccaggttg cggagggtga
      781 tgtttcccgt aacggtatga ggcggactgc cgtacacctc gccctggact tcataataga
      841 tctgaacatc ggtcagcagg ttatcagcgg ggaccaccga cacatcactg gcggccagtt
      901 cgcccatatg gacgacgccc cagccgttgg catcttcgcc aaaccagtgg gccgtcaccc
      961 cgttgccgaa aggcccctgg aaaaccatcg ggccgcctga gccacccgtg aagtcagtcg
     1021 cggcgacgag cataatccct tgcggaaaag taatatagat ctgcttgatc cattcattgt
     1081 cgttgcccgc gttgtaagtc ctgaaattcc agacatattc ctggcccgca tggaaccggt
     1141 catggtctgt gaccaggtac gatccttcga tattccggtt cccttttccg tctttcacca
     1201 cggaaggctg tattcccgct atgtcggccg tgaaattcaa cggccctgag gccggattcg
     1261 acaaagccag cgatttcgtt tcggtctcac ccgtccggag caggaaatcg accgatgagg
     1321 ggctgaattg caagccgggt gagcaattga ccgacgatgc aaacgagatc aggtcaaccc
     1381 acgcagcatc catccggtaa ttggtgttgt gatctttatg atagatccat tcgaaccggt
     1441 gaaagccttc cgtgacggga aattctgcat acgaccaacc cgtttcgcca tcccatctct
     1501 ccatctcatt tccatcaatt ttaaaactca ggtaatcgta attatcggcg gaagtcgtat
     1561 ccccttcaca actggtccgc tggtaaaaac tgatgacacc atccgtcaaa taatccatat
     1621 cgatgaccat caccgattcc tgattgtgtg taatattgcc cgagcgggct gcgaatgtac
     1681 cctcgtaagg ggcataggta tcagtcctcc actgcccgtc gccttcgtgc ccccaggtga
     1741 acaactccat atcaccggat tcaaagtttt cgctgtataa tccgacaacc agtgagaatg
     1801 tgtcggtagt cgcatagttc ccgtcaccgc tgatattcag cgcaaattcg atttcgtggc
     1861 ccggcaggca atcgggcgaa acgaagacgt tgtaactgag attggccagt attccgactt
     1921 ccagcaacgg gatattaccg accgactggt tgatcatgac cagcggatcg gccgtcgtca
     1981 gcaatgtatt gacactggta agctctacgc ctcccgtgtt caggtaggaa actacgacat
     2041 cggaggtttc gccggcatcc agcctgccat tttcatcgtt tacttctacg ttttcggctg
     2101 ttacgacggg agcataggca taatggtaca attcgctgat ccagacgttt gtgtcagaag
     2161 tgatcgaggt ttcaacaatg atcaggcgct cgttgggaat atcgctggcg acggtaaact
     2221 ggaatgcgtt gagaacattc cgcggcaggt tgaacgccag gtcgcccagg ttttccgtat
     2281 tgtcggtaac ggtaatgtaa ggatcatcga tggtgatggt catgaccgca tcgggaatgg
     2341 tttccggctt caggttcagc accgagatgc tcatattggc ggtttcgctg aatgcgataa
     2401 cgggatcgcc gccagaaatg acagaatcgc tgatgattat gccggtagat gtaaaagtga
     2461 aggtcttttt tgtttggatg aagttgttat cggttacttt gaatgtgatc tgttcattgc
     2521 cgtaggattg ttcaggcgtt cccgaaaaaa gccccgtttc gctcaacgtc atctcctccg
     2581 gatagtcata cgggaccaat gtagtggagg tattggcatt gaccgacggt ttgtttgata
     2641 accttgtctc ctgaaagttg aatccatctc cgtcgccgat gccggccatc cagaggtaca
     2701 tatcgcagcc gttcatctgg ccgtagtaga attttatggt cccgtcggga tagagacggg
     2761 ctgcatagtt gaaagtcatc gccgggtttt cggtcagggc cgttttccag cggaaagtca
     2821 cgtaatctgc gctgccttcg taccagatgc cgcacccctg gccggcattg atctcctggt
     2881 ggtagttcag gaaaggggag atgtggcggg tgatgttgaa cagcaccttg tcgtcctgat
     2941 agtaagggaa aggataaagc tgctgatcga acatcaggta accgtcgaca tgcagcgtga
     3001 tgctggaata agcgctgtcg tagaaaggaa aggagaacgg aatggcctgg gtgacgtacc
     3061 cgctggaact gttgccgggc gtcaattgtg ttccggtgat ttgcgggaaa gaggccggct
     3121 ggcctgactc atcgtaactt ttatcaaatt cccagatgta aggaggggtt ccgcccgcca
     3181 cggtgaactg atggctgtaa tattctccct ccggggccgg cgggatcgtt tcgtcgacaa
     3241 tgtggacgcg gtcgaaatta aaggtgtggt tcagataaag cgttgtcgta tcatcgttta
     3301 ccagcggcac attcgtctgc gcacaaacga cctgggagcc gcctgatgtg tggtcaaaga
     3361 tggaaaaatg gatgacttcg cctgtattcg cattgtcggg atcattctcg acaacctgga
     3421 ggtaaatctt aaccggatcg ccgatatcgg aataacttaa gagtttcgac aggtcgagcc
     3481 cgatttcgat ggtcttgtcg gcttcggtgg ttcctccctg catgtaccgg ttgccgccct
     3541 ggtagtcata gatcgggaag cccagttggt gctcgggtct ttgtgcaccg acgttattgg
     3601 caataccggc cagaactttt atccgttccc ggcggtcgtg cttcaggatg atctttgccg
     3661 tcagtttcgg atcgagggcc cctttgacat cgaggacatg gacgcagtga ttccagatac
     3721 cgccgctgcc caggttatcg gccacggttt tgtacatcat gtaagcaaat ccctggtcgc
     3781 cccagttcgg gacaccgccg taactctgca ccatcttgaa ccctccgatc tcccagtctt
     3841 tcatgtttac cgtcccatcc ccgttgatgt cgatgttatt cgtgtacagc ccgtcgctgt
     3901 tgtagtccca gcggatgctg tcgttgtaac cgacgatagt catggcatgg ttggcgcttc
     3961 caccgaaata ggtcagtaca tatttgcctg cctcgggtgt tccccagggc agcatggtgg
     4021 tgacggccat atgctgggca taaaagctgg ccacaccacc cacagccgat ccctccaggt
     4081 ggttgtgcag ccagtgtttg agcaccagca gcccttccgg tgtgcccacc tggatctggt
     4141 atgcatttgt gatccggttg tgcatgccgt tgtagtattc gttgtagccc gacaggaatc
     4201 gggatgctcc gccgtatgat aaagagccgc cgtaatcaac ggcattcatc atcccgtatt
     4261 ctttcaggat ctggatgctg tggagatagc ttacaccgta ccatccttcg ccgccgttca
     4321 tgaagttcca ggcaaaatgc gtggggtact ggttttggag caggctggca ttcagattgc
     4381 gttcccgcgc catctcatag gtaaaattgt acccgataca tgatgcttgt ccgcagctga
     4441 gtccggcttg ctgataggcg ggacgcatat acatttgtgt agaattgtcg accatgtagg
     4501 gcagatcacg gttttcaaat cctgcaggcg gtttcagctc cggaagcgac atcagttgca
     4561 cctcatctac tgcagatagc ggccgtagga tgtaggtctc ctccggcttt tctacctggg
     4621 cttgaagatt attaaataag accgtgatag cggctaccag gataagtcga agtttgattc
     4681 gcatagtttt ttattgaggc tttaaagata gataaaatta catttaaggt tgaaggttga
     4741 gtgtttgatt gtaattcgtg ctgaagctgt ctcaaaagtc tgttatttcg atttgtttgc
     4801 ctgaaaacag ttcgtgctcc aacgactaaa gccgttggtt ccgaatgttt tatgagggtt
     4861 ggaaccgacg gctttagtcg tcggactaca aagtttccac aataaccgat gggtttgccc
     4921 gtcggaagaa aaaaacatta accgacggtt ttagtcgtcg gactgcttag tttccacaat
     4981 aaccgacggg ttcacccgtc ggactatcaa gctcccttcg gagtgcaatc tgtttttttt
     5041 ttaacttcgt cgcctcaatt gaatagcatg tttgagaaga ttaaagaagc ggccggatat
     5101 ctgaggtcaa aagggatggt ggcgcccgag gtgggaataa tcctcggcac cgggctgggc
     5161 aaactggccg atcatatcga tgtggagata accattgatt atgaggatat tccccatttt
     5221 cccatttcca ctgtcgaatt tcaccacgga gttttgatat acggtaacct gcgggggaga
     5281 aaagtgctgg tgatgaaagg ccgttcgcat gcttacgaag ggtataccac gcagcagata
     5341 acttttccca ttcgggtcat gaaaatgctg ggaatcagca gcctgctggt ctccaacgcc
     5401 tgcggttcga tgaacccggc tttcaaaaag ggctcgctga tgctgatcga agatcatatc
     5461 aacctgttgc cgaatccgct tgtcgggccg aatatcgatg aactgggtac ccggtttccc
     5521 gatatgagcc ag
//
LOCUS       DAOPNH010000238         5507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17134_length_5507_cov_6.460382, whole genome shotgun sequence.
ACCESSION   DAOPNH010000238 DAOPNH010000000
VERSION     DAOPNH010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17134_length_5507_cov_6.460382"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKZ74_11515"
     CDS             <1..242
                     /locus_tag="PKZ74_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPM93424.1"
                     /translation="NPGFGGQSFIEGTLDKIQKLKEMMALKNTTAIIEVDGGVTLENA
                     PRLVKAGAQVMVAGQTVFGSPDPQKVISSLKKLTA"
     gene            complement(243..1694)
                     /locus_tag="PKZ74_11520"
     CDS             complement(243..1694)
                     /locus_tag="PKZ74_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93425.1"
                     /translation="MKQFIAFIFGSICFLFFTVNVNSQWAFNGTHIYNTNAGNVGIVT
                     NAPGTLLDVAKSMTEPTIRVYNLGGNGGATYQMKDALSGADWKFKATNLGGFKIRDHA
                     LSLDVMVIEQNSAANCIYIQQGGNVGIGTNSPLEKLDVNGAVRIGNTANSNAGAIKWD
                     GTNFLGYNGTSWINLDFTWNDPWILAPNVPVGNPEFSSGPQPMSLSFAFPGIPNPTAR
                     LYLTDMSAPGIFPHLLTIETITAGAAALNASQLFRISAGMNPPFNDYSLGIFGGDGTF
                     KLTFGSTLLPTAQGDNSTMIRSYPGGIIDFPNQSRVRAYQLDPNGFVQVIPPNIWTPV
                     NFTNDAPLIAGYDEQNEFTVAPAANMAVPPEMAYFTALTEGYYQVNARCEFAVYMAQV
                     MPGSYISIAIYTGPVPGATGPYAIGNNLAIATFGNMGEPVPLEWNNAPNVSDVVYLMP
                     GQIISIWVYHNALNPLNLIQGPDKCYVSIHKVS"
     gene            1845..3302
                     /locus_tag="PKZ74_11525"
     CDS             1845..3302
                     /locus_tag="PKZ74_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPM93426.1"
                     /translation="MSDIYHLKPERLWYFFGEVLKIPRPSKKEEKIIQYLIDFGQNHQ
                     LETIKDEVGNVVIRKPGSHGYENAPWVCLQGHVDMVCEKDADIIHDFDKDPIQAYVED
                     GWLKARGTTLGADNGIAVATMLALLEAKDIAHGPLECLFTIDEETGLTGAFGLDENLL
                     KSRILLNLDSEDDGEFFIGCAGGKDTVIEMNLIKEDIPAGYQTMRMKIFGLQGGHSGD
                     DIHRNRGNANKILNRFLWESAQKFGIRLCSFDGGNLRNAIAREAIGIFLVPAGKTNQV
                     AEFAAGYLEQVRFEFRVSEPDLNLEIEEFETPGFVMAKSTQEYLLNALYACPHGVIAW
                     SQDIPNFVETSTNLASVKMQNGKIRIATSQRSSIESAKEDICNMVASVFKLTGASISQ
                     SDGYPGWAPNPNSKVLELCVSTYEEMFGQKPVVRAIHAGLECGLIGDKYPGMDMISYG
                     PTMRGVHSPTERLEISTVDKFWVFSLELLKKLALV"
     gene            complement(3299..3880)
                     /locus_tag="PKZ74_11530"
     CDS             complement(3299..3880)
                     /locus_tag="PKZ74_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93427.1"
                     /translation="MVYNRMKTIWLTVFLILCLVPDMISQDTVRMPGRRARSDFWERV
                     DVGGNLGAQFGRVTVLNIEPRISYDITDHLYAGIGVTYLFYRDKRYAPAISLSYYGGS
                     IFGGYYIWRDLYAHVEYAPLYIPDFYDYFIPPIQGMEDQPPWAHDIYLGGGYRQWIGE
                     KIYVNLMILFNINETIYSPYSNPIIRIGFGAGL"
     gene            4010..5488
                     /locus_tag="PKZ74_11535"
     CDS             4010..5488
                     /locus_tag="PKZ74_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93428.1"
                     /translation="MDTKRIPTAFLLLVTGTLFMMSIMVSCEKDPIDHSPKVMLEFSQ
                     DTVFFDTVFTSLGSVTLPLIVHNRGDNRISISSIRLAGGGSSFYRMNVDGDPVFEAKD
                     VEIAANDSLYIFCRVTVDPTDTRNPYVVSDSIIFETNGNIQDVNLVAWGQNVHYILAD
                     SYIPGFPKYKIIAHEGETTVWTADRPYVIYGFAVVDSTGRLEIQRGAKIHFHNNSGLW
                     IYKGGTLKVKGSPDAPVIFQGDRLEEFYKNLPGQWDRIWINEGSVNNEIDYAIIRNGF
                     IGLQLETLQEQMGNQLILSNTRIENMTGYGLLTRFYNLTAFNNVVTECGQYLAAFTWG
                     GYYDIRHCTFANYWSTSVRVTPAIVLNNFAVDQDDVVYPFEFQGYFGNNVFDGRNEDE
                     LLIQGDAGAPFNYRLDHCLLKTVQDISNPEHFTQCLVNQDSLYLDLFNGNYQPDTLSP
                     LVDRGNPAVLTESFFDLSRDIIEGNREKSLPDIGAYEFIKPE"
BASE COUNT         1491 a         1357 c         1297 g         1362 t
ORIGIN      
        1 tcaatcccgg attcggcgga cagagtttta ttgagggtac cctcgacaag atacagaagc
       61 ttaaggagat gatggccctg aaaaatacca ctgcaattat tgaggtcgat ggcggggtga
      121 cattagaaaa cgcgccccgg ctggtaaaag ccggagcgca ggtgatggtg gcgggccaaa
      181 ccgtctttgg atcgcctgat ccgcagaagg ttatctcctc tttaaaaaag cttaccgcct
      241 gatcagctta ctttgtgtat ggagacataa catttatccg gaccctggat caggttcaac
      301 ggattcaatg cattatgata aacccagatt gaaatgattt gtccgggcat tagatatacc
      361 acatcagaaa cgttcggtgc attattccat tccagcggaa caggttctcc catattgccg
      421 aatgtagcga tcgccaggtt attcccaatt gcatagggcc cagttgcacc aggcaccgga
      481 ccggtgtaga ttgcaatgga tatataggat cccggcataa cctgcgccat atacactgcg
      541 aattcgcatc gcgcattcac ctggtagtag ccttctgtca gcgctgtaaa atatgccatt
      601 tccggtggaa cggccatatt tgcggcaggt gcaacggtga attcgttttg ctcatcataa
      661 cccgcaatca atggcgcatc gtttgtaaag ttgaccggag tccaaatatt cggcggtata
      721 acctggacaa acccgttcgg atccagctgg taagcccgta ccctgctttg gttgggaaaa
      781 tcaattatac cgcccggata agaacggatc atcgttgaat tatcgccttg cgctgtaggt
      841 aacaacgtcg acccgaaagt taatttaaat gtgccatcac caccaaagat ccccaggctg
      901 taatcattaa atggaggatt cataccggca gaaatgcgga aaagctgtga cgcattgagt
      961 gcggcagccc ctgcggtgat ggtctcaatc gttaacaggt gaggaaatat tcccggagcg
     1021 ctcatatcag tcaaataaag cctggccgtt ggatttggaa tccccggaaa tgcgaatgaa
     1081 agcgacatgg gttgcggacc ggaggaaaat tccggatttc ctaccgggac gttaggtgcc
     1141 agaatccatg gatcattcca tgtaaagtca aggttaatcc agctggtccc attatagccc
     1201 aggaaattgg tcccatccca cttaatcgct ccggcattgg aattggccgt gttaccgatc
     1261 cttacggcac cgttcacatc cagcttttcc agagggctgt tcgtacctat gcctacattg
     1321 cccccttgct ggatgtagat acaatttgca gcagaattct gctcaatgac cataacatcc
     1381 agacttaacg catgatcacg gattttaaaa ccacccaggt tggtggcttt aaacttccag
     1441 tcggctcctg aaagcgcatc tttcatctgg tatgtcgccc cgccgttccc tccgaggtta
     1501 taaacccgga tggtcggttc tgtcatgctt ttggcaacat ccagcagagt tcccggcgcg
     1561 ttggtgacaa tacccacatt tcctgcattg gtattgtaaa tatgcgtgcc gttaaacgcc
     1621 cactgactat ttacatttac ggtaaaaaac agaaagcaga tgctcccgaa aataaaggcg
     1681 atgaactgtt tcatggtgag ttttttttaa ttgataatga agactatgac ccaaacaaat
     1741 atacgaaaaa gaaatagata tgtcaaggaa ttcaccattt attttcaata ttcattgcat
     1801 caggaaaata ttaactttac agcattaaaa caacatgcaa aacaatgagt gacatctatc
     1861 atttgaagcc cgaaaggcta tggtactttt tcggcgaggt attaaagatt ccccgccctt
     1921 caaaaaaaga agagaagatc atccagtacc tgatcgattt tggtcagaat caccaactgg
     1981 aaacgatcaa agatgaagtt ggcaatgtcg tcatacgcaa gccgggttca cacgggtatg
     2041 aaaatgcacc ctgggtatgc cttcaggggc atgtcgatat ggtgtgtgaa aaagatgccg
     2101 acatcatcca cgatttcgac aaagatccga tccaggccta tgtggaagat gggtggctga
     2161 aagcgcgggg cactaccctt ggcgctgata atggaatagc tgtggccacc atgctggccc
     2221 tgcttgaagc aaaagacatt gcacacggcc cgctggaatg ccttttcacc atcgacgaag
     2281 aaaccgggct gaccggtgcg ttcggcctgg acgaaaacct cctgaaaagc cgtatcctgc
     2341 tgaacctcga ctcggaggac gatggagaat tcttcatcgg ttgtgccgga ggaaaagata
     2401 ccgtcatcga aatgaatttg ataaaagaag atattcctgc cggataccag accatgagga
     2461 tgaaaatatt cggtttgcag ggcggacatt cgggcgacga tatccatcgc aacaggggca
     2521 atgccaataa aatcctcaac cgtttcctct gggaatcggc tcaaaagttc ggaatcaggc
     2581 tctgttcctt cgacggtgga aatctccgca atgctatagc ccgcgaagcc atcggaatct
     2641 tcctggtgcc ggccggcaaa acaaaccagg tggcagaatt cgctgccggc tacctggaac
     2701 aggtccggtt cgaattcagg gtatcagaac ccgatctgaa cctggagatc gaagaattcg
     2761 agacacccgg gttcgtcatg gcaaaatcga ctcaggaata tcttctgaat gctctctatg
     2821 cttgtccgca tggcgtaatt gcctggtcgc aggacatccc gaactttgtg gaaacctcca
     2881 ccaacctagc ctctgtaaaa atgcagaacg gcaaaatcag gatcgctacc agccagcgga
     2941 gttccatcga atcagccaaa gaagatatct gcaacatggt cgcttcggtg tttaagctga
     3001 ccggagcctc catcagccag tccgacgggt atcccggctg ggcgcctaat ccgaattcca
     3061 aagttctgga actttgtgta agtacctatg aagaaatgtt cgggcagaaa cctgtggtca
     3121 gggctatcca tgccggcctg gaatgcggcc tcatcggcga caaatacccc gggatggata
     3181 tgatcagtta cggaccgaca atgcgtggcg tccactcccc taccgaacga ctggaaataa
     3241 gcactgttga taaattctgg gtattctcgc tggagctgct caaaaagctg gccctcgttt
     3301 agagacctgc gccgaatccg atccggatga taggattgct gtacggcgaa taaatcgtct
     3361 cattgatatt gaaaaggatc atcaggttca cgtagatctt ttcaccgatc cactgcctgt
     3421 accccccgcc caggtatata tcatgtgccc agggcggctg gtcttccatt ccctgtatag
     3481 gaggtataaa ataatcatag aaatccggaa tataaagcgg agcatattcc acatgagcgt
     3541 aaagatcccg ccagatgtaa taacctccga aaatgctgcc cccataataa cttagtgata
     3601 tagccggggc ataacgctta tccctgtaaa ataaataggt caccccgata ccggcataca
     3661 gatggtcggt gatatcatag ctgatccggg gctcgatatt caacactgtc acccttccaa
     3721 actgggcgcc caggttcccc ccgacatcca cacgctccca gaaatcactc ctggctcgcc
     3781 gcccgggcat tcgcaccgta tcctgcgaaa tcatgtcagg aacaagacaa aggatcaaaa
     3841 atacagtcag ccagatcgtt ttcatcctat tgtagaccat taccggatcg tttgatttga
     3901 aacggtaaat ttaccaatat atttttccgg cgatcacgat tgcacaataa agccggcctt
     3961 attccgtata ttgatggaga atcgctaatt ttgcccgaaa tttcaattaa tggacactaa
     4021 aagaatcccc accgctttcc tgttactggt cacgggcact ctttttatga tgtccattat
     4081 ggtttcctgc gaaaaagatc cgatcgacca ttctcctaaa gtaatgctgg aattttcgca
     4141 ggatacggtt ttcttcgaca cggtatttac cagccttggt tcggtaaccc ttccattgat
     4201 tgttcataac cggggagata acaggatcag catctcttct atacgcctgg ctggtggagg
     4261 ctcctctttt taccggatga atgtggacgg cgacccggtt tttgaagcca aagatgttga
     4321 aattgccgcc aacgacagtc tctatatatt ttgccgcgtg acggtagatc cgaccgatac
     4381 ccggaatccc tatgtcgttt ccgactcgat catcttcgaa acgaatggta acatacagga
     4441 tgtcaacctg gtagcctggg ggcaaaatgt ccactacatc ctggccgact cctacatccc
     4501 ggggttcccg aaatacaaaa taatagccca cgaaggagaa accacagtat ggacagccga
     4561 caggccttac gtcatctacg gttttgccgt agtcgattca accggcaggc tcgaaattca
     4621 aagaggggct aaaatccatt tccacaataa ctcgggattg tggatctata aaggtggaac
     4681 gcttaaagta aaaggttcgc ccgatgcccc ggtcattttc cagggcgaca ggctcgagga
     4741 gttttataaa aatttacccg gacaatggga ccggatatgg atcaacgaag gcagcgtaaa
     4801 caatgagata gattatgcca ttatccggaa cggttttatc ggattgcagc tcgagactct
     4861 tcaggagcaa atgggcaatc agttaatcct ctccaatacc cgtattgaaa acatgaccgg
     4921 gtatggtctt ctgacccgct tttataatct gaccgcgttt aacaatgtgg tgacggaatg
     4981 cgggcaatat ctcgctgctt ttacctgggg aggttactac gatatccgcc actgtacctt
     5041 tgccaattac tggtcgacct ctgtcagggt aaccccggct attgtcctga acaatttcgc
     5101 ggttgatcag gatgatgtcg tgtatccttt tgaattccag ggttattttg gaaataatgt
     5161 atttgacgga aggaatgaag atgaactgct gatccaggga gatgccggcg caccgttcaa
     5221 ttaccgcctg gatcactgtc tcctcaaaac cgttcaggat atctccaatc ctgaacactt
     5281 cacccaatgc ctggttaacc aggattcgct ttaccttgat ctgttcaatg ggaattacca
     5341 gcccgacact ttatcaccat tggtcgatcg cggaaacccc gccgttctta ccgaatcttt
     5401 ttttgatctc agcagggata ttattgaggg taacagggaa aaaagcctgc ccgatatcgg
     5461 cgcttacgag tttattaagc cggaataagg gttccaggtt ccaggtt
//
LOCUS       DAOPNH010000239         5450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17373_length_5450_cov_6.754958, whole genome shotgun sequence.
ACCESSION   DAOPNH010000239 DAOPNH010000000
VERSION     DAOPNH010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17373_length_5450_cov_6.754958"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKZ74_11540"
     CDS             complement(<1..563)
                     /locus_tag="PKZ74_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM93429.1"
                     /translation="MMKKISSFVDYFQQYHESVTNPEGYWAKIADTFQWHKKWDKVLD
                     WNFTEPKIKWFDGGKLNITENIFERNLPARKDQVALIWEPNDPKEPEKRITYGELFEK
                     VCIFANALKRQGIQKGDRVAIYLPMVPELPIAMLACARIGAIHSIVFAGFSANSLADR
                     INDSACKMVITADGLYRGSKNVGLKSIV"
     gene            complement(628..1869)
                     /locus_tag="PKZ74_11545"
     CDS             complement(628..1869)
                     /locus_tag="PKZ74_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93430.1"
                     /translation="MKKPLLLLFLAYFPVMLFSQDDKTFGIKFTGFVKTDVIWDSRQT
                     VDLREGHFLLLPKPVLNDLEGNDINAKPSFNFLSIQTRLKGDITGPDALGAKTSGSIE
                     GEFFGMADGDINGFRLRHAYVKLNWKTTELLVGQMWHPMFITSSFPEVVSFNTGAPFL
                     VFSRNPQIRLTKDIKSFRLILTALSQVDFKSNGPDGASTKYIRNSSIPSFNLNFEYSY
                     KNKETGTEILAGAAGNFKRLLPRMITDSGYQTNEGINSLSGMVYLKLGFQKVIFKMAG
                     LYGQNMFDYTMIGGYVVDDYLDKDKGIVSYRNITTASVWAEVMTTGKKMQGGLFLAYS
                     QNMGAGDIVIGPYYSRGANIYDLYRISPRFVYNAGKFRIAPEIEYTVARYGMTNNLDG
                     KVMDPEPVGNFRLLVGVFYFF"
     gene            complement(2102..2845)
                     /locus_tag="PKZ74_11550"
     CDS             complement(2102..2845)
                     /locus_tag="PKZ74_11550"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPM93431.1"
                     /translation="MSGHNKWSTIKHKKGAADAKRSKIFSKLVKEIQVAVKEGGPDPD
                     HNARLRLVIQNAKGVSMPKDNIERAVKGGNKDAASFFEVTFEGYLPNAVAVFIECTTD
                     NQQRSVSNIRSIFNKYNGNLGTNGSLSFLFERKGIFSIPKENYNADEHELELIDAGAE
                     DISDDGDYITITTAMDDFGKMQKKMEELKIVAENARLQRIPNDYKKLGLEETKKILKA
                     IETFEEDDDVQNVYHNMEIPDEYVDQLED"
     gene            complement(3235..4785)
                     /locus_tag="PKZ74_11555"
     CDS             complement(3235..4785)
                     /locus_tag="PKZ74_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPM93432.1"
                     /translation="MKTMQNTRGSFLGVLISILFMITTTAFSQKEIVRETRDLPAFNQ
                     IDAGGAFQFILVPGTPQKVGVEARQKDIGKIATEVKDNKLSVTGKGISNNPVLNVYIT
                     MPSLTRLEAHGAADVESEGTFKEEMMSLEVSGAASVDLSLDVKTLSSDVSGAADLELT
                     GKAERHSTVVSGAGTLNAFDLKTVTTDAEVSGAGTAKVSAGELTGESSGAGKIYSDDG
                     NWAGNDTKSIVEVDDDGDTTYIKVPGTSVIISEGDDSVKVKVGNRVIIIDDDGNVKTR
                     HHKWPKFNGHWAGVDIGLNGFVTPDFNMNLPREYEYLDLRMEKSIAVNLNFFEQNIPL
                     SKNQKWGIVTGLGLGFNDYRFLRPTRLSVDSSFLVGYIDKGIEIRKSKLSAMYLTLPV
                     LFEFQTAPVYHNNGFHINVGVVVGARLSSHTKKYYEDLNAEFDVTQYNPATGMYETVF
                     TAISPEQHKTRVYDDYFLQPFKFDATARIGWNFLNFWASYSLNSMFREGKGPELYPWG
                     AGITLVCF"
     gene            complement(4850..>5450)
                     /locus_tag="PKZ74_11560"
     CDS             complement(4850..>5450)
                     /locus_tag="PKZ74_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93433.1"
                     /translation="LLLMTVSRFWPEPLPEELVPDETPSALAETPSRIKETPSQIKET
                     PSALPETPSQIKETPSHRARMESRIIEMESQFLTPLLTRNIAIDQNPVLLQASLDYHP
                     LPENIMPETEEKGLLASVVSNLFGKARDAFRENPTFERLSETDLNLWAIAQAGVNGYN
                     RISDRDLALYVHKDQDGNVLSYALVEQDKLLLSKHLDKE"
BASE COUNT         1379 a         1253 c         1275 g         1543 t
ORIGIN      
        1 acaatcgatt tcagtccgac attcttggaa ccacggtaca aaccatcggc ggtaatgacc
       61 attttacagg cggaatcgtt gatgcgatcg gccagcgagt tggcggaaaa tccggcgaag
      121 acaatgctgt ggatggctcc gatacgggca caggccagca tcgctatggg aagttccgga
      181 accattggca gataaatggc taccctgtcg cccttctgga taccttgcct tttcagtgca
      241 ttggcaaaaa tgcaaacctt ttcgaataat tccccgtacg tgatcctctt ttccggttct
      301 ttgggatcgt tcggttccca gatcagcgcc acctggtcct ttcgggccgg cagattgcgc
      361 tcaaaaatat tctccgtgat attcagtttc ccaccgtcga accacttgat cttgggctcg
      421 gtgaaattcc aatccagcac tttatcccat tttttgtgcc attgaaaagt gtcggcgatc
      481 tttgcccaat acccctcggg attggtcacg ctttcatgat attgctgaaa ataatcaaca
      541 aaacttgaaa ttttcttcat catacgctta tgatttaaat ttgtaaataa tgattaggat
      601 atttagatgt tattgggttt caatttttta aaagaaataa aatacaccta caagcaagcg
      661 gaaattaccg accggttccg gatccataac tttaccgtcg agattgttgg tcatgccgta
      721 tctggcaacc gtatattcta tctcaggggc aatcctgaat ttgcctgcat tataaacgaa
      781 ccggggtgat atccggtaga ggtcataaat attcgctcct cttgaatagt aagggcctat
      841 cacaatatcg ccggcaccca tgttctgaga ataggccagg aataaccctc cctgcatttt
      901 cttgcccgtc gtcatcacct cggcccacac cgatgccgtg gtgatattcc ggtagctgac
      961 gatccctttg tctttgtcca ggtaatcatc caccacataa cccccgatca tggtataatc
     1021 gaacatgttc tggccgtata atcctgccat tttgaaaatg accttctgaa aacctaattt
     1081 caaatatacc atccccgaaa gggaattaat accttcattc gtctgatagc cactatcggt
     1141 gatcatcctc gggaggagcc gtttgaaatt accggcggcg cctgccagga tctccgtgcc
     1201 ggtttccttg tttttatatg aatactcaaa gttgaggtta aaagagggta tggaggaatt
     1261 tcggatgtat ttcgtgctgg ctccgtcggg gccgttgctt ttaaaatcga cctgtgacag
     1321 ggcagtgaga atcaggcgaa aacttttaat gtcttttgta agtctgatct gaggattgcg
     1381 ggaaaagacc aggaaagggg caccggtatt gaaagaaacc acctccggga atgaggaagt
     1441 gataaacatc gggtgccaca tctgcccgac cagcagctcg gtagttttcc agttcagctt
     1501 tacgtaggca tgacgcaggc ggaaaccgtt aatatcacca tcggccatac cgaaaaattc
     1561 gccttcgatc gatcccgagg tttttgcacc cagtgcgtca gggcctgtta tatctccctt
     1621 gagccgggtc tgtatgctga ggaaattaaa actcggcttg gcattgatat cgttgccttc
     1681 caggtcgttt aatactggtt tgggaagcaa aagaaagtgc ccttccctca aatcgaccgt
     1741 ttgccgcgaa tcccagatga catctgtttt aacgaacccg gtaaacttta ttccaaatgt
     1801 tttatcatcc tgcgagaata acatgacagg aaagtaagcc aggaaaagta aaaggagagg
     1861 tttcttcatt cgggattttc ttgttaattg attaacaata aaagatttat tacgggcgaa
     1921 gatagaattt tttttgactt aaacaagcca ttagcaggaa gggaagatag gtatccgaaa
     1981 tagtaaagat cggatattca atacattatt atatagttta aaaactatat gaattaatta
     2041 ccccgaaatt taaaagcatt ataaataaga agcgtgtagc gccgttttac cggtttggtt
     2101 atcagtcttc aagctgatcg acatattcat ccggaatttc catattatga tagacattct
     2161 ggacatcgtc gtcttcttca aaggtctcaa tggctttcag aatctttttg gtctcctcca
     2221 ggccgagttt cttgtaatcg ttgggaattc tttgcaaacg ggcattttcg gccacaatct
     2281 tcagctcttc catctttttc tgcatcttgc cgaaatcatc catagccgta gtgatcgtaa
     2341 tataatcacc gtcatcggaa atatcttccg caccggcatc gatcagttcg agttcatgct
     2401 catcggcatt ataattctct ttaggaatgg aaaagatgcc tttgcgttcg aacaggaagc
     2461 tcagcgaacc attggtaccc aggtttccgt tgtatttatt gaaaatggag cggatattgc
     2521 tgaccgatcg ctgctggtta tcggtagtac attcgataaa aacagccacg gcattgggaa
     2581 ggtacccttc gaaggtcact tcgaagaagc tggcggcgtc tttgtttcca cctttgacag
     2641 ccctttcgat attatccttg ggcatactga ctcccttggc attctggatg accagcctga
     2701 gccgggcatt gtggtcggga tcggggccgc cttcttttac ggcaacctgg atttctttta
     2761 cgagcttgga gaaaatcttg gagcgcttgg catctgctgc ccctttcttg tgcttgatcg
     2821 tagaccattt attgtgtccg gacatacgct gggaattttg agttttgagt tttaaatttt
     2881 aatttattgg tattaaatac tttaccctaa aatgttgcgg gttgtaaaat taggaaaatt
     2941 ttctgaacaa aaaagccaat gataattaaa gacattggat tggtaataaa agaaagtgag
     3001 tgtatcaaaa gtctgatatt tcgaattgat agcctgacaa tagtttgtgc tccaacgact
     3061 aaagccgttg gttccaagga gttgcaattg gaaccgacgg ctttagtcgt cggaaggaaa
     3121 acccatccag aaccgacggc ctcaaatccc taccatactg gcactcttat ggagttcaga
     3181 ggaattttaa ctttggaacc tggaacctgg aacccgaaac ctggaacctg gaacctagaa
     3241 acacaccagc gtgatcccgg ctccccaggg atacagttcc ggtcctttcc cttccctgaa
     3301 catcgaatta agcgaataac tggcccagaa gttcaggaaa ttccatccga tccgggcagt
     3361 tgcatcgaat ttgaaaggtt gcaggaaata atcgtcgtaa actctggttt tgtgctgttc
     3421 cggtgaaata gccgtgaaaa cggtttcata catcccggtt gcgggattgt attgtgttac
     3481 gtcgaattcg gcattcaaat cctcatagta ttttttggta tgcgatgaaa gcctggctcc
     3541 gaccacgact ccgacgttga tatgaaaccc gttgttatga taaacgggag cggtctggaa
     3601 ttcgaacagg acaggcaacg tcaggtacat agctgaaagt tttgatttcc gaatctcaat
     3661 gcctttgtcg atatagccga ccaggaatga tgaatcgacg ctcaagcggg ttggccgcag
     3721 gaaccggtag tcattgaatc ccaggccaag gccggtgaca atgccccact tctggttttt
     3781 agacaaaggg atattttgtt cgaagaagtt tagattgacg gcgatggatt tttccatgcg
     3841 caggtcgagg tattcgtact cgcgcggtag gttcatgttg aagtcgggag tcacaaagcc
     3901 attcaggcca atatccacgc cggcccaatg tccgttgaat ttgggccatt tgtggtgtct
     3961 tgttttgaca ttgccatcat catcaataat gatcacccgg ttaccgacct tcactttgac
     4021 ggaatcatcg ccttccgaaa tgatcacgct ggttcccggt actttgatat aagtagtgtc
     4081 gccgtcatca tccacttcaa cgatggactt ggtgtcattg ccggcccagt ttccgtcatc
     4141 cgaatagatt ttgcccgcgc cgctggattc cccggttagt tcgccggcgg aaactttcgc
     4201 tgttccggcc ccgctgactt cagcatcggt agtgactgtt ttcaggtcaa aggcattgag
     4261 tgtgccggct ccgctgacca ccgtgctatg ccgttccgct ttgcccgtca gttcaaggtc
     4321 tgccgctcca gatacatcag agctgagggt tttgacatcg agcgacaaat cgacggatgc
     4381 cgctccggac acttccagcg acatcatctc ttctttgaat gtcccttcac tctcaacatc
     4441 cgcagctcca tgagcttcca gccgggtcag ggaaggcatg gtaatgtaaa cattaagtac
     4501 ggggttattg ctgatccctt tgcccgtgac gcttagttta ttatctttca cttcggtagc
     4561 gattttcccg atatcctttt gccgggcttc gacccctact ttttgtggag ttcccggaac
     4621 caggataaat tgaaaggcgc ccccggcgtc aatctgatta aaagccggaa gatcgcgggt
     4681 ttccctgacg atctctttct ggctgaaggc ggttgttgtg atcatgaaca aaatgctgat
     4741 aagcactccc aaaaatgatc ctcttgtatt ttgcatcgtt ttcatataaa aatagtttat
     4801 aagttttttt gttgtgacgg gcagggtaat gaaaatgtta caggcaatat tattctttat
     4861 ccagatgttt ggataacaag agtttatcct gctcaacaag ggcataggac aacacatttc
     4921 catcctgatc tttatggaca tacagcgcca ggtcgcggtc ggagattctg ttgtatccat
     4981 tcactccggc ctgcgcgatc gcccacagat tgagatcagt ttccgaaagc ctttcgaagg
     5041 taggattttc gcggaaggca tcgcgggcct tgccgaagag attggagacc acgctggcca
     5101 ggagtccttt ttcttcagtt tccggcatta tattttcagg taaggggtga taatcaagcg
     5161 aagcctggag caatacgggg ttttgatcaa tagcaatatt gcgggttagg agcggggtca
     5221 ggaattgaga ctccatctca attatccgcg actccatccg ggcacggtga gatggagtct
     5281 ccttgatttg agatggagtc tctggaaggg cagatggagt ctccttgatt tgagatggag
     5341 tctccttgat ccgagatgga gtctcggcca gggcagatgg agtctcatcg ggaaccagtt
     5401 cctccggcaa aggttccggc caaaaacggg atacggtcat taataacaat
//
LOCUS       DAOPNH010000240         5408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17551_length_5408_cov_8.466841, whole genome shotgun sequence.
ACCESSION   DAOPNH010000240 DAOPNH010000000
VERSION     DAOPNH010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17551_length_5408_cov_8.466841"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1147)
                     /locus_tag="PKZ74_11565"
     CDS             complement(101..1147)
                     /locus_tag="PKZ74_11565"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPM93434.1"
                     /translation="MSRRKITVAVTALNAIDSPGPGVSVIRGLKESKIYDVRVVGLAY
                     ESLEPGIYMHDIVDRVYQIPYPSAGVESLFARLEYIHSRENLEVIIPNFDAELFPFIR
                     LSERFKKLGIRMLLPTEHQFEERHKINLPEFGKKHGIKVPGSVPAYQGAEIFKAAETI
                     GFPMLVKGKYYDAHVAFTIEHAREYFNKISAKWGLPIILQKYVKGVEYNVIGLGDGEG
                     NRIAAVPIRKQYITDKGKAWGGISVSDETMLKLTDHFIESTKWRGPFELELMKDEKNE
                     FSLLEINPRIPAWVYLAIGVGQNIPEAIVSLALGEKVEPFKSYEVGKMFIRYSWDMIV
                     DLKEYQQISTYGER"
     gene            complement(1134..1388)
                     /locus_tag="PKZ74_11570"
     CDS             complement(1134..1388)
                     /locus_tag="PKZ74_11570"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPM93435.1"
                     /translation="MKIKSNIALSESGFIFNPNTGESFTLNPTGQLLFGLIREGKEFP
                     EIREYFLENFETEEDVFEKDFEDFIHLLNSYQMMEPDEQA"
     gene            complement(1504..1932)
                     /locus_tag="PKZ74_11575"
     CDS             complement(1504..1932)
                     /locus_tag="PKZ74_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93436.1"
                     /translation="MSTGERSFNENLDLLIRLLRKMKDKSQFEGIPGVPKSFVANFEF
                     FIQNYERMKDQIAAQLLQQFGEPIKQMVADMVDQLKEELDEDPMLATPDEPASPAEIP
                     VITGQKKTVEEIDEMLRKPGLSEEEIDRLLDERKKVKSEE"
     gene            complement(2178..3542)
                     /locus_tag="PKZ74_11580"
     CDS             complement(2178..3542)
                     /locus_tag="PKZ74_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPM93437.1"
                     /translation="MKTGPINRTIFLNVWLLLIVLHSNNSFAQKDVIRLTFTAIDIAG
                     HIQLDSIKVMNRTQGGDTVLFWPDTVLSLNYFGITEINNPQEAFRVFQNYPNPVANRT
                     TISLFVPEMDHVILQVSEPTGRVIFKSDRILNQGVHTYEFTPGPGNLYFFTAKWRESS
                     GNIKILSSGFNLNRICTLEYTGTGISSPSFKVANGIQSLPFNPGDELLYIGYTGTLES
                     GMLDSPVESTIDTFQFSTNIPCPGTPTVDYEGQVYNTIQIFSQCWLKENLNVGTMIPG
                     AQNQMNNSIIEKYCYNNEPDSCNKYGGMYQWNEMMQNTTQQGAHGICPPGWHLPTEQE
                     WRVLQGAVDSEFGIEDGAWGFDGFSGFDAGTNLKTTSGWIENGNGSNLFGFSGLPGGS
                     RTYAGNVFGLVGSAAFWWTSMQGPEDSGWYRYIENHWQQVGRHYFTKEMGFNVRCLQD
                     SKTY"
     gene            complement(4106..4390)
                     /locus_tag="PKZ74_11585"
     CDS             complement(4106..4390)
                     /locus_tag="PKZ74_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93438.1"
                     /translation="MKSIYTFVKGKSYPVNPGLPGGRDLVQRTRIAQAGPEDCSLSIV
                     HCPLFIVHCPLSMAEGKIRKSNVENRKSTIIFPARGDTPTVTFNVCHSIQ"
     gene            complement(4908..5342)
                     /locus_tag="PKZ74_11590"
     CDS             complement(4908..5342)
                     /locus_tag="PKZ74_11590"
                     /inference="COORDINATES: protein motif:HMM:NF039408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HPM93439.1"
                     /translation="MNTRKTLQNEKINVKIILSALWVAVMFIYVYADIKTLFQPEIPE
                     QIISGIVGGMTINQSFLFAAAILMSIPTIMIILSLTLKPSINRWVNIIVSFLHIILII
                     ITRFVPGKIWYYYIYYQSIEAIFHFLIILYAWRWPIQEKQTV"
BASE COUNT         1361 a         1290 c         1229 g         1528 t
ORIGIN      
        1 gttcaccctt cactgttcac ccttcactgt tcacccttca ctgttcaccc ttcaccgttc
       61 acccttcact gttcaccctt cactgttcac cgttcaccct tcaccgttca ccatacgtcg
      121 atatctgctg gtattccttc aggtcgacga tcatatccca cgagtagcgg atgaacattt
      181 tgcccacctc gtatgatttg aatggctcga ccttttcgcc gagggccagg ctgacgatcg
      241 cttcggggat gttctgcccg acgccgatgg ccaggtaaac ccaggccggg atgcggggat
      301 tgatctccag gagcgagaat tcgttctttt cgtccttcat cagctccagc tcgaacggcc
      361 cgcgccattt cgtcgactcg atgaaatggt cggtcagttt gagcatcgtt tcgtcgctca
      421 ccgaaatgcc gccccacgct tttcccttgt cggttatata ttgtttgcgg atcggcacgg
      481 cggcgatgcg gttgccctct ccgtcgccca gcccgatcac attgtattcc acccccttca
      541 catatttctg gaggatgatc ggcaggcccc actttgcgct gatcttgttg aagtattccc
      601 gggcatgttc gatggtgaaa gccacatggg catcgtaata tttccccttg accagcatcg
      661 ggaagccgat cgtttcggca gctttgaata tctctgcgcc ctggtaggcc ggcacgctgc
      721 cgggcacctt gatgccgtgc tttttgccga attccggcag gttgatcttg tggcgctcct
      781 cgaactggtg ttccgttggc agaagcatgc ggatgcccag ctttttgaac ctttccgaga
      841 ggcggatgaa cggaaacagc tccgcatcga agttggggat gatcacctcc aggttttccc
      901 gggaatggat gtattccagg cgcgcaaaaa gggattcgac accggcggat ggatacggta
      961 tctggtatac ccggtcgacg atgtcgtgca tgtagatgcc cggctcgagc gattcgtaag
     1021 ccagtccgac gacccggacg tcgtaaatct tcgattcttt cagcccccgg atgaccgaca
     1081 ctcccggccc ggggctgtcg atcgcattca gcgccgtaac ggctacggtg atcttacgcc
     1141 tgctcatcgg gttccatcat ttggtagctg ttcagcaggt gaatgaagtc ttcgaaatcc
     1201 ttctcaaaga catcctcttc ggtctcaaaa ttttccagga aatattccct gatctccgga
     1261 aactctttcc cttcgcggat cagaccgaat aaaagctgcc ccgtgggatt cagcgtaaac
     1321 gactcgcctg tgttcggatt gaagatgaag cccgactcgc tcagggctat gttgcttttg
     1381 attttcattt atcgaggaac ttaaatataa ccttaaccgc acaacacccc ttttacccta
     1441 ccccagattc attgtccatt gtccattgtc cattgtccat tgtccagttc cacccttcac
     1501 tcttcactct tcactcttca ctttcttccg ctcatccagc aggcggtcga tctcttcttc
     1561 ggagaggccc ggtttgcgaa gcatctcatc gatctcctcc acggtctttt tctgcccggt
     1621 gatgaccggt atttctgccg gggaagccgg ttcatccggg gtggcgagca tcgggtcttc
     1681 atccagctct tcctttagct ggtcgaccat atcggccacc atctgcttga tcggctcgcc
     1741 gaactgctgc agcaactgcg cggcgatctg gtctttcatc ctttcatagt tctggatgaa
     1801 aaactcgaag ttggcgacga aagattttgg aacgccgggg atcccttcga actgcgactt
     1861 atctttcatc ttccggagaa gcctgataag caggtcgagg ttttcattaa aggaacgttc
     1921 gccggttgac ataggggaaa tttttttgca aaggtaaggg gtttttcggg atttggcgat
     1981 ttggcgattt ggcgatttga agatttgaag atttgaagat ttgaagattt gaagatttga
     2041 agatttgaag atttggattt ggaatttgtt tggaatttgg agctcggaat ttggaatttg
     2101 gtccgacagc tgatcactgg atgaattggc agacattgaa ggtaacggtt ggcgcatcgc
     2161 cccgggccgg aaaaatgtca ataagtctta gaatcctgaa gacagcgaac attaaaaccc
     2221 atctcctttg tgaaatagtg tcggcctact tgctgccaat ggttttcgat gtaacgatac
     2281 catccgctat cttcggggcc ttgcatcgat gtccaccaga aggcggcaga gccaactaaa
     2341 ccaaaaacat ttccagcata agtacgggag ccgccaggca gacctgagaa accaaaaaga
     2401 ttggagccgt tgccattttc aatccagccg ctggtggtct tcaggttggt accggcatca
     2461 aatccactaa atccatcaaa gccccatgca ccgtcttcaa ttccaaactc gctgtccacc
     2521 gccccttgaa gcactcgcca ttcttgttcc gttggcagat gccagcccgg gggacatata
     2581 ccgtgcgcac cctgctgggt ggtgttttgc atcatctcgt tccattgata catcccgccg
     2641 tatttgttgc aactgtcagg ttcgttattg tagcagtact tttcgatgat actgttgttc
     2701 atctggtttt gggctcccgg aatcattgtt cccacattca ggttctcttt aagccagcat
     2761 tggctgaaga tctggatggt gttgtacacc tgtccttcgt aatctaccgt tggtgttcct
     2821 gggcagggga tattggtgga gaactggaaa gtatcaatcg tgctctccac cggggagtca
     2881 agcatacctg attccagggt gcctgtatag ccaatataaa gcagttcatc gcccggattg
     2941 aaaggtaaac tttgaatgcc gtttgcaact ttaaacgaag gggaagaaat cccagtgcca
     3001 gtatattcaa gtgtacagat cctgttcaaa ttaaaaccag aactaagaat tttaatatta
     3061 ccgctacttt ccctccattt tgcggtgaag aaataaagat tcccaggccc gggagtaaac
     3121 tcgtaggtat gaacaccttg attcagtatc ctgtcggatt tgaaaattac acggcccgta
     3181 ggttctgaaa cttgcaaaat aacatggtcc atttcaggta caaacaagga tattgtagtt
     3241 cgatttgcaa cagggtttgg ataattctga aagacccgga atgcctcctg tggattatta
     3301 atctcggtaa ttccaaaata attaagactt aatactgtgt caggccagaa gaggactgta
     3361 tcacctcctt gcgtgcggtt catcacttta atactgtcaa gctggatatg accagcaatg
     3421 tcaattgctg taaatgttaa tctgatcaca tctttttgag caaatgaatt attgctgtgc
     3481 aggacaatta gtaaaagcca gacattcaaa aagattgttc gattaattgg tccagttttc
     3541 atatatcaaa gtatgttggg taactttcct gtgcagcgcc cccgttcggg caaggctggg
     3601 ccgggttaat ttgcacgggc aggcggcggc gcgaggatgt aaagttagat attagtaatt
     3661 taaattgcaa acagatccct ccttcgtacc aatgacatgt aagtaaattt ctaatgaact
     3721 gctctccgga ggtgagccct atttgtagca ataagtacgg gaaagaaaaa aagcccctcc
     3781 ctttggaaat attggaatat tgcgaaaaac cttcacaggg aggggttggg gagggaaacc
     3841 agaaatgcat tttcccctcc ccttaatccc ctcccatgct gatattacct tgaaaaactg
     3901 gattatcgcg ggaggggaaa attgtgcggg tgctatctcc tttgctacaa atgtggaact
     3961 cctccggagt aatgaaaagc taatgtcatc caatattgtg gagtttgtaa aagtctgaaa
     4021 actcgggagg acgcagttgg ccaatggaat tttcggcttc agatgtgcgc tgatagctgg
     4081 aaaacttctc ccgccaggga tgaaatcact ggatagagtg gcagacattg aaggtaacgg
     4141 ttggcgtatc gccccgggcc gggaaaatga ttgtcgattt tcgattttcg acgttcgatt
     4201 ttcgaatctt cccttcggcc atcgacaatg gacaatgaac aatgaacaat ggacaatgaa
     4261 caatggacaa tgaacaatcc tctggtccgg cctgggcgat gcgcgtccgt tgcactaaat
     4321 cccgtcctcc cggtaaaccg ggattgacgg gatagctttt tccctttaca aacgtataaa
     4381 ttgatttcac cacaaaactt ttatactatg gaaaattcct aacaagaaac cttcatgcag
     4441 ccgttataaa ttcaacgaat agaggccgtc tcaaaagtcc atatattcac ttttttaggt
     4501 agtttgacga ctgaaaccgt cggttccaac cctcataaaa cattcggaac caacggcttt
     4561 agtcgttgga gcacgaactg ttttaaggca atcaaatcga attatcagac ttttgagaca
     4621 gcctctccca ctttagaaac cttaaattca ataattgctt ttagtaaact gcgcgggacg
     4681 ggatgttccc acatgggcgg gacaagctgt gcaacatggc ttcatagcct gccccgattt
     4741 atcggggtta ggctacgcca cacgaagcct tattttattt gtggctgtaa atatttattc
     4801 ctctccgcaa ttgtccgctg ctattacgag gaaagaatat ctagagaaac gattgtatcg
     4861 aacgaaaaga caactatcac ttgcatcgac tttgccaatc ttatcggtca taccgtttgt
     4921 ttttcttgaa ttggccatct ccacgcgtac aaaataatta gaaaatgaaa aattgcttcg
     4981 attgattgat aatagatata gtagtaccat attttgccag gcacaaaacg tgtaatgatt
     5041 attagtatta tgtgcaggaa actaacaata atgtttaccc atctgtttat gctcggcttt
     5101 agtgtaagcg agagtataat cataattgta ggaatagaca ttaatattgc agctgcaaat
     5161 aaaaaacttt ggttaattgt cattccaccc acgattcctg aaataatctg ctcgggaatc
     5221 tcaggttgaa aaagtgtctt aatgtccgca taaacataga taaacattac agcaacccac
     5281 aaagctgaca atattatctt tacattaatc ttttcatttt gaagtgtttt tcttgtattc
     5341 ataattatta tcatttaagg gtacatctaa atactaaatt gttaataaga atgtttatat
     5401 ctacttgt
//
LOCUS       DAOPNH010000241         5391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17636_length_5391_cov_9.115255, whole genome shotgun sequence.
ACCESSION   DAOPNH010000241 DAOPNH010000000
VERSION     DAOPNH010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17636_length_5391_cov_9.115255"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1488)
                     /locus_tag="PKZ74_11595"
     CDS             complement(97..1488)
                     /locus_tag="PKZ74_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM93440.1"
                     /translation="MNETEEKIKARILIVDDDKDVLLAARLFLKQHVEVVHTEENPEN
                     LPNLINSENYDMVLLDMNFAPDATSGKEGFYWLNKILGIDPSIVVILITGYGDIELAV
                     QGIKEGATNFLLKPWENRKLLATIKTSLQLRQSKVELQDLRTKQKVLMADQNQPYETI
                     IGTSQAMQKVLLQAQKVAVTEANVLILGENGTGKELIARAIHRASARAGEVFISVDLG
                     AISESLFESELFGFKKGAFTDAKEDRPGRFEAAHKGTIFLDEIGNLSLALQSKLLSVL
                     QNRNVVRLGTNKEIPIDVRLVCATNMPLYQMVKEGKFRQDLLYRINTVEVHVPALRER
                     LDDIPLLIDHFLEIYCKKYKITQKRVAEDTLARLKKHDWPGNIRELQHSVERAVIMSD
                     SGILQPHDFFITGKEEDKSDAIDLNNLNLEEAERMLVRKVVDKHGGNISKAAKELGLT
                     RASLYRRIEKYGL"
     gene            1668..2918
                     /locus_tag="PKZ74_11600"
     CDS             1668..2918
                     /locus_tag="PKZ74_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPM93441.1"
                     /translation="MDRIIEKKKWTVKKILQYTAILVFAIFLIYIFLLRDRSSRLTID
                     KTQITTDKVALSNFQEFIPVDGIVLPKTTIYIDAVLGGTVETKYVEDGAEVKAGDMIL
                     KLSNANMQLSYMDQETRMYDAINNLQNSRIALEQNKYIRQREITNLLYQIDQVTTEFE
                     RAKKLYNEGVYTLKEYEDAKRDFDFSSKQLNISLELQRLDSISAEDQKRQIAASVDRM
                     KTNLALLQSNMERLLVLAPADGVLSSFNVEIGETRSAGERLGQIDIMDGYKMRANIDE
                     RYVSRVVPGQEAELDFGGKTYQLYVYKIYTGVTGGAFQVDMMFRDEVPANIKRGQTIQ
                     MRLQFSTPTEALILKRGGFFQQTGGNWVYVVDPSGKYAVKRNIKLGRQNTRFYEVLEG
                     LEAGEEVIVSSYDSFGEKDKLVLR"
     gene            3055..3318
                     /locus_tag="PKZ74_11605"
     CDS             3055..3318
                     /locus_tag="PKZ74_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93442.1"
                     /translation="MREIVIIVPDFEPEQNVEIEVTINGRKKVIHYRVELVNFEAEGV
                     PPKDKVTVLKHKIAEHEKDWELMEIGAPQGENVPLMFRRKVNV"
     gene            3422..>5391
                     /locus_tag="PKZ74_11610"
     CDS             3422..>5391
                     /locus_tag="PKZ74_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93443.1"
                     /translation="MLYVRDELSFDRHYRNSDRIARVCMIYDFGGVGENSASMPFPVA
                     YTLKSEYPDLVENVVRIFNFQSDRSLVEYEGKKFSESRFYFADSSFFEIFSQEFIKGD
                     PATALLEPHSVVITEKMAEKYFPGEDPYGKILKYEVNLPLKVTGVVKEPVSQTHFRFD
                     FIASLSTMKKVFNGRLPQTWVWNPCWTYILMPEGAQPARLEEKFPAFIQKYYYDAQRE
                     SIVMYLQKLTDIHLHSRIDYEIRPNSNYMYVVVLSAIAVFLLLIAAINFMNLATATAG
                     SRAREIGIRKVTGADRMRLIGQFLGESLAMTLLAFILALILIQAILPSFNDLTGKDIQ
                     TGYLADPINLAWLAGLWLLLGLLSGAYPALFLSSFKPIAILKPGLFINSRSGLPRKIL
                     VVFQFTISIGLILSTMMIFRQVNFLRNAEMGFDPENIIMVRINQTKVSKDFETFRNEL
                     IRNPNIESVTALDDIIGESHNTHEFWFEGMKEKEWRFFPALVVRYDFLQTFGIELVAG
                     RDYNRENKTDPEQALLVNEALVKHMGWSSNDEALGKRFHSLNGDEKIVGVFRDFQPTS
                     FREPAGPFILNMKEKAWEINFFMKYAAIRTTGKNNAEAIRYIESKWSEFEKEKPFDYF
                     MLKDELKRLYNDERNLGNLAVALHQQLVVRQNV"
BASE COUNT         1326 a         1360 c         1407 g         1298 t
ORIGIN      
        1 gtgaacttgt accgggggat ttccagcagg aagaaaaagg ccagcagggt cagggccatc
       61 aggatgaccc gcagggcgat ggcgatccgg aagcggctaa agaccatatt tttcgattct
      121 tctgtacaaa gatgcgcgtg tcagccccag ttcttttgcg gctttgctga tattgccccc
      181 gtgcttgtcg actactttcc tgacgagcat ccgttcggct tcttccaggt tcaggttgtt
      241 aaggtcgatg gcatcgcttt tatcctcttc tttaccggtg atgaaaaaat catgcggctg
      301 gaggatgccg gaatcgctca tgatgactgc tctttcgacc gaatgctgca attcgcggat
      361 attcccgggc cagtcgtgct ttttcagccg ggccagcgta tcctccgcga cccgcttttg
      421 ggtgatctta tattttttac agtagatttc gaggaaatgg tcgatcagca gcgggatgtc
      481 atccagccgc tcgcgcaggg ccggcacatg aacttcgacc gtattgatgc ggtacagcag
      541 gtcctggcgg aatttcccct ccttcaccat ctggtacaat ggcatgttgg tggcacacac
      601 cagcctgacg tcgatcggga tctccttgtt ggtgccgagc ctgacgacgt tgcggttctg
      661 caaaacgctc agcagcttcg attgcagggc cagcgacagg ttgccgatct cgtcgagaaa
      721 gatagtgcct ttgtgggccg cttcgaacct tccggggcgg tcttccttgg catcggtgaa
      781 agcccctttc ttgaagccga agagttcgct ctcaaacaac gactcgctga tcgcgccgag
      841 atcaacgctg ataaaaactt cgccggccct ggcggaggcg cggtggatcg ccctggcgat
      901 cagctcctta cctgttccgt tctcccccag gatcagcaca tttgcttcgg tcaccgcaac
      961 tttttgcgcc tgcaggagca ccttttgcat tgcctgcgaa gtgccgatga tcgtttcata
     1021 gggctggttc tggtcggcca tcagcacttt ctgcttggtg cgcagatcct gcagttcaac
     1081 cttggactgt cgcaactgca gtgaggtttt gatggtagcc agcaacttcc ggttctccca
     1141 cggcttgagg aggaaattcg tcgccccttc cttaatcccc tggaccgcca gctcgatatc
     1201 gccataaccg gtgatcagga tcaccacgat cgacggatcg atcccgagta tcttgttcag
     1261 ccagtaaaat ccctctttcc cactggtggc gtcgggggcg aaattcatgt cgagcagcac
     1321 catgtcgtaa ttctcgctgt tgatgagatt cggcaggttt tccggatttt cctccgtgtg
     1381 caccacttcc acgtgctgtt tcaggaacag ccgggcggcc agcaatacgt ccttgtcgtc
     1441 atccacaatc aggatccggg ctttgatttt ttcttcggtt tcgttcatag gtcaataatc
     1501 cctcattatg aaggcataaa attataaata atgttcacca gtgtacaaaa aagtgaacga
     1561 atttgaacac atctaataaa attacctgtt ttacgaatct gtaaatcagc atcgtatttt
     1621 tagtggcacg aatttggttc atgccgctta ccccaaaacc agactacatg gaccgaatca
     1681 ttgaaaagaa aaaatggact gttaaaaaga ttcttcaata cactgccatt ttagtttttg
     1741 caatattcct catctacatt ttcctgctca gagaccgttc cagccgcctc actatcgata
     1801 aaacccagat cacgaccgat aaagtcgcct tgagcaattt ccaggagttc atccctgttg
     1861 atggtattgt tttaccgaaa acaacgattt acatcgatgc ggtgctgggt ggtacggttg
     1921 agacgaaata tgtggaagac ggcgctgaag tgaaagcagg ggatatgatc ctgaagctga
     1981 gcaatgcgaa catgcagctt agctacatgg accaggagac gcggatgtac gatgccatca
     2041 acaacctgca gaattcacgg attgccctgg aacagaacaa atacatccgg cagcgggaga
     2101 tcaccaacct cctctaccag atcgaccagg tgaccaccga gtttgagcgt gcaaagaaac
     2161 tttataacga aggtgtttat accctgaaag aatatgaaga cgccaaacgc gattttgatt
     2221 tttcgagcaa acaattgaat atcagccttg agctgcagcg cctcgattcg atctcagcag
     2281 aagaccagaa acggcagatc gcagcctcag tcgaccggat gaaaaccaac cttgccctgt
     2341 tgcagagcaa tatggaacgg ctgctggtcc tcgccccggc cgatggtgtg ctctcttcct
     2401 tcaatgtgga aatcggcgaa acccggtcgg cgggagaacg gctcggccag atcgatatca
     2461 tggacggcta caagatgcgg gcgaatatcg atgagcggta tgtgtcgcgc gttgtgcccg
     2521 ggcaggaagc agagcttgat tttggcggaa aaacgtatca gctctacgta tataagattt
     2581 acaccggggt gacaggcggg gctttccagg tcgatatgat gttccgggac gaggtaccgg
     2641 caaacatcaa gcggggtcag acgatccaga tgcggttgca gttcagcacc ccgaccgagg
     2701 cgctcatcct gaaacggggt ggcttcttcc agcaaaccgg cggcaactgg gtctatgtcg
     2761 tggatccttc gggaaaatat gcagtgaaaa gaaatattaa actgggaagg caaaataccc
     2821 ggttctacga agtgctcgaa ggactggaag ccggcgagga ggtgatcgta tcgtcgtatg
     2881 attcgttcgg ggagaaggat aagctggtgc tcagatgagc agtcagttgg cagttggcag
     2941 ttggcggtcg tgaggcgttg gtcatcaatt taacagttta acagtttaac aatagaacaa
     3001 ttgaacaatt gaacaattga acccggaatc ctgaaacctg aaaaactctt ctttatgcgc
     3061 gaaattgtca ttattgttcc cgactttgag ccggaacaga acgtagagat cgaagttacg
     3121 atcaatggcc ggaaaaaggt catccattac agggtagaac tggtcaattt cgaagcggaa
     3181 ggcgttccgc cgaaagataa agtcaccgtt ctgaaacata agatcgccga acatgaaaaa
     3241 gactgggaac tgatggagat cggcgctccg cagggagaga acgtaccgct gatgttcaga
     3301 aggaaagtca atgtttaaca atttcataag gattttgctt cggggcttcg tcaagcgaag
     3361 ggtttacacc gttataaacc ttctcgggct tgcgattggc ctaacgagtt tcatcctcat
     3421 catgctttat gtaagggatg aactgagctt cgaccgccat taccggaact ccgaccggat
     3481 tgcccgcgtc tgcatgatct atgacttcgg cggcgttggc gagaattcgg ccagcatgcc
     3541 ttttccggta gcctatacac tgaaatctga gtatcccgac ctggtggaaa atgtcgtcag
     3601 gatcttcaat tttcagtcgg accgcagcct ggtggaatat gaaggaaaga aattcagtga
     3661 aagccgcttc tattttgccg attcgagttt ctttgaaatc ttctcacagg agtttatcaa
     3721 aggggatcct gctacggcgc tcctggagcc tcattcggtt gttattacgg aaaagatggc
     3781 ggagaaatat ttccccggtg aagatcctta cgggaagata ctgaagtatg aagtcaacct
     3841 gccgttgaaa gtgaccggcg tggtgaaaga accggtatcc cagacccatt tccggttcga
     3901 tttcatcgct tcgctcagca ccatgaaaaa ggtgttcaac ggccgactgc cccagacctg
     3961 ggtgtggaat ccgtgctgga catacatcct gatgcctgaa ggtgcacagc cggcccggct
     4021 ggaggagaaa ttcccggcgt tcatccagaa atactactac gacgcccagc gggagagcat
     4081 cgtgatgtac ctgcaaaaac tgaccgatat tcatcttcat tcccggatcg attatgagat
     4141 caggcccaac agcaattaca tgtacgtggt cgtgttgtcg gccattgcgg tatttctgct
     4201 gctgatcgcc gcgatcaact tcatgaacct ggcaacggcc acagccggct caagggcccg
     4261 ggagatcggt atccgcaaag tgaccggcgc cgaccggatg cggctgatcg ggcagttcct
     4321 cggcgaatca ctggccatga ccctgctcgc gtttattctc gccctgatcc tgatccaggc
     4381 catccttccc tccttcaacg acctgacggg aaaagatatc cagaccggtt acctggccga
     4441 ccccataaac ctggcctggc ttgccggtct ctggctcctg ctgggactgc tatcaggcgc
     4501 ataccccgcc ctgttcctgt catctttcaa gcccatcgcc attctgaaac cgggcttgtt
     4561 catcaactcc cggagcggtc tgccccggaa aatcctcgtg gtcttccagt tcaccatttc
     4621 catcggattg atactgagca cgatgatgat attccggcag gtaaacttcc tgcgtaatgc
     4681 cgagatgggc ttcgatccgg aaaacatcat catggtcagg atcaaccaga ccaaggtgtc
     4741 gaaggatttt gagactttcc gtaatgaact gatccgaaac ccgaatatcg aaagcgtaac
     4801 ggccctcgac gatatcatcg gggaatcgca caatacgcat gaattctggt ttgaagggat
     4861 gaaagagaag gaatggcgct tcttccccgc cctggtggtc cggtatgatt tcctgcagac
     4921 cttcggcatc gaactggtag cgggaaggga ttacaaccgg gaaaacaaga ccgatcccga
     4981 gcaggcgctg ctggtcaatg aagcgctggt aaagcacatg ggatggtctt ccaatgatga
     5041 ggcgctgggt aaaaggtttc attcccttaa cggggatgag aagatcgtcg gcgtgttccg
     5101 cgatttccag ccgacctctt tccgtgagcc ggccggtccg ttcatcctga atatgaaaga
     5161 aaaagcatgg gaaatcaatt tcttcatgaa gtacgccgcc atccggacaa caggtaagaa
     5221 caacgccgaa gcgatccgct atattgaatc gaaatggagc gaatttgaaa aagagaagcc
     5281 ttttgattat tttatgctga aggatgagct gaagagattg tacaacgatg agcgcaacct
     5341 cggcaacctg gctgtggctc tccatcagca acttgtagtt aggcagaatg t
//
LOCUS       DAOPNH010000242         5382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17687_length_5382_cov_7.608035, whole genome shotgun sequence.
ACCESSION   DAOPNH010000242 DAOPNH010000000
VERSION     DAOPNH010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17687_length_5382_cov_7.608035"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PKZ74_11615"
     CDS             complement(<1..982)
                     /locus_tag="PKZ74_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM93444.1"
                     /translation="MKSNFLKLLSLLFSPSPFLLLSLFPLLLFAQTPLPSNWDSIVAS
                     PWYTGNVSNIPAFCYLNPEDPDTGYFFIADKALPQVIPVSVKWKKGVPRSAAFTYQSD
                     KIKVKFTGTVSPDTIAGRIRTSRKSAIRLGISPELEILLIKEIPQIPQSPNLQIPKSP
                     NPPTSLLPRYLSPIFKKVTVTKDIAYGAAPGHYVSMPIEKDSDYDYQQIILDALKNMW
                     VNPGKKALLHLFNRDPVSFAVSDLQGLKMDLYEPEGDTLSFRPLILLLHGGAFILGDK
                     ATESIAELANDYARKGYVVASINYRIGFNPASKSSLERAAYRSVQDARAAL"
     gene            complement(1099..1671)
                     /locus_tag="PKZ74_11620"
     CDS             complement(1099..1671)
                     /locus_tag="PKZ74_11620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPM93445.1"
                     /translation="MKNNLILLVLLSLSTFLSAQEIKTKEPIKPALLVIDIQNKFLPM
                     MDAKDREMGMLYIKGYIDIFRRHNLPVICIHHTDPKEGPFPGTPEFAFPDDIGIAKDD
                     PVVVKTYANGFTKTDLDKVLKEKGVNTLFLCGLSSVGCVLATYIGATDHDYKAFLLKD
                     AIFSHKSEYTDQVETIFDAVGWDVVKLAIE"
     gene            1846..3342
                     /gene="crtI"
                     /locus_tag="PKZ74_11625"
     CDS             1846..3342
                     /gene="crtI"
                     /locus_tag="PKZ74_11625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPM93446.1"
                     /translation="MKTVAIIGAGAAGLSAANYLAIKGFRVTIFEKNAFPGGRCASFE
                     KEGHRFDIGATLLMMPQVYERMYADFGRNMRDELDLIRMDPVYRLRYADGSELKFTSD
                     LMRMQDQLEAMEPGSYTAFLRYMDKSFKAYKVSMETIIDRNYDNIFQFINPVNLFRLF
                     RLNAFGNHYRTSARYFKNENLRTAFTFQNIYVGQNPFEAMAIFSMLPFMELTDGVYFP
                     KGGMNRVSESLEKIAIGNGVEIQYRANVSGIRVAGERVAGIELADGTFYEADIVLANA
                     DLPYVYQELLPSGPQANRIKKLGYTCSAIVFHWGMDTVLPGIEQHNVYVSDNYRNNIE
                     EVFNGSGFAPEPSFYLHSPARADKSAAPEGHDSISVIVPVGHMGNGKFGDLGIDWEVK
                     KEEARAAVLKRLAMEGFADFEKHIKFEKVYNPVIWKNMFNLSKGATFGSLNHNLMQMG
                     YFRPHNQHNKYKNLFFAGGSTHPGNGVPLALISARLTAEKIIGVNGER"
     gene            3365..4336
                     /locus_tag="PKZ74_11630"
     CDS             3365..4336
                     /locus_tag="PKZ74_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HPM93447.1"
                     /translation="MNNLETIIAETFSKIDFHKIKDHPNILIAARFWEDERYEAAATI
                     YRFMRYIDDMIDDRKALDGMLSCMEKKIYTDQVNAWIDCLGLDRTNDPFFGEVTETIR
                     RFRIPLHFFYNFARSMVYDINHDGFRTFDDFLDYAEGASNGPASVFVHLCCLDKQQGE
                     YVPINLSISDVARPCAIFSYLVHIVRDFQKDQFNNLNYFALDLLEKHGLTAGDLREIA
                     RGSAIPDGFRELIRDYKEQAGKYKTDTEKMIRSLEGRLDHRHMLSLRIIYHLYLQIYN
                     RIDPDNGTFTMAELNPGPEEVKEAVAECIRVNFSERTPKVCPEIHPH"
     gene            complement(4364..>5382)
                     /locus_tag="PKZ74_11635"
     CDS             complement(4364..>5382)
                     /locus_tag="PKZ74_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPM93448.1"
                     /translation="FDQNPDALILNCERPDLADILESSDLSRIKMIFGDKKIIALDEA
                     LKIRNIGPLLKLVYDEPSFVQKIIATGSSSFELANTISEPLTGRNVKFLLLPLSIGEL
                     SGARGWLWIYENLNLMPIYGSYPGIIDISGEFKTRKLFDLASDYLFKDILIHENLRNP
                     SLLRKLLKLLALQVGSQVSFHELSKTLAVSSKTIEKYIDLLEKSFVIFSLPSFSRNQR
                     TEIRKSRKFYFYDLGIRNALITNFNDPDSRTDTGAIWENFCILERLKYNLIHQPFAQL
                     YFWRTYDGAEIDLVEEANGKINIFEFKWRGKSNARFPRSFSENYEINTGKLISKENIH
                     ELIA"
BASE COUNT         1431 a         1122 c         1400 g         1429 t
ORIGIN      
        1 ggagggcggc ccgtgcatcc tgcaccgacc ggtaggctgc ccgctccagg gagctcttgc
       61 tggccgggtt aaagccgatg cggtaattga tggaggcaac gacgtaaccc ttacgcgcat
      121 aatcatttgc cagctctgcg atggattcgg tggctttatc gccaagaatg aaggcgccac
      181 cgtggagcag gaggatgagt ggccggaagg ataaagtatc gccttcaggc tcataaaggt
      241 ccatcttcag cccttgcagg tcggatacgg caaaggagac cggatcacgg ttgaacaggt
      301 ggagcagcgc ttttttcccc ggatttaccc acatattctt caaggcatcc aggatgatct
      361 gctggtagtc gtaatcgctg tctttctcga tcggcatgct cacatagtgg ccaggcgcag
      421 cgccataagc aatatctttg gtaactgtga cttttttgaa gataggcgaa aggtatcgcg
      481 ggaggagaga ggttggggga tttggtgatt tggggatttg gagatttgga gattggggga
      541 tttggggaat ttctttaatt aaaagaattt caagttcagg gctgatgccc aggcggatag
      601 cggatttgcg ggaggtgcgg atcctgccgg cgatcgtgtc gggactgact gtaccggtga
      661 atttaacctt tatcttatcg gattggtatg taaatgccgc cgaccgcgga acacctttct
      721 tccattttac agacaccggg atcacctggg gaagcgcttt atcggcaatg aaaaaatacc
      781 ccgtatcggg atcttcggga ttaagatagc agaaagccgg tatattgcta acatttccgg
      841 tgtaccaggg tgaggctacg atgctgtccc agttagaagg aaggggagtc tgggcgaaga
      901 ggagaagtgg gaacaaggag agaaggagaa atggagacgg ggagaagagg agactcagta
      961 attttaagaa attggatttc atgatgttat aaaatgtaat aatcagagtg aaaattagcg
     1021 attttattat tccactgaat ttttcgaaca cgaattaaac gaatataacg aatgaacacg
     1081 aagagtattc aggaagaatc attcaatcgc cagttttaca acatcccagc ccacggcgtc
     1141 gaagatcgtt tcgacctggt cggtgtattc ggatttatgg ctgaatatag catctttcag
     1201 aaggaatgcc ttatagtcgt gatcggtggc cccgatatag gttgccagca cgcaaccgac
     1261 tgagctgaga ccgcagagga acagcgtatt tacccctttc tctttcaaaa ccttatccag
     1321 atcggttttg gtaaagccat tcgcgtaggt tttcacgacc accggatcgt ctttcgcaat
     1381 accgatatca tccggaaagg caaactccgg ggtgcccgga aagggtcctt ctttcggatc
     1441 ggtatggtgg atgcagatga cgggcaggtt atgccggcgg aagatatcga tataaccttt
     1501 gatgtaaagc atgcccattt cccggtcttt tgcatccatc atggggagga acttattctg
     1561 gatatcgatg accagaaggg caggtttgat aggttctttt gttttgattt cctgtgcgga
     1621 aaggaaggtg ctgagagaca gcaggacgag tagaatgagg ttatttttca tattgaataa
     1681 tattaaatgt aaaaatagtg ttttctcgca aattaatgct aatttttcgc ggattcccgc
     1741 aaatttatat ctgcgttcat ttgcgctgca atctgcgaaa atcagcgtga aataaaaact
     1801 ctgccatttt tgtaaccttt cgcttttttc ctgtcctttg cgaatatgaa aacagtagcg
     1861 atcataggcg cgggggcggc aggcctgtct gccgctaact atttggccat taagggcttc
     1921 agggtaacaa tatttgaaaa gaacgctttt ccgggcgggc ggtgtgccag tttcgaaaaa
     1981 gagggtcacc gcttcgacat cggagccact ttgctgatga tgccgcaggt gtacgaacgg
     2041 atgtacgccg atttcgggag aaacatgcgc gacgagctgg atctgatcag gatggatccg
     2101 gtgtaccgcc ttcgttatgc cgatgggtcg gagctgaaat tcacctccga cctgatgcgg
     2161 atgcaggacc agctggaggc gatggaaccg ggaagctaca cggcttttct gagatacatg
     2221 gacaagagct ttaaagcgta caaagtttcc atggagacca tcatcgaccg gaactacgac
     2281 aacatctttc aattcatcaa cccggtgaac cttttcaggt tgttccggct gaatgctttc
     2341 ggaaaccatt accggacatc tgccaggtat tttaagaatg aaaacctgcg gaccgctttt
     2401 acttttcaga acatttatgt agggcagaat ccatttgagg cgatggctat tttttcgatg
     2461 ctgcctttca tggagctgac cgatggggta tattttccga aaggggggat gaacagggta
     2521 tcggagagcc tggagaagat tgctattggg aatggtgttg aaattcagta ccgggcgaat
     2581 gtgtcgggga ttcgggttgc aggcgaacgg gtggcaggca ttgaactggc cgacggtacc
     2641 ttttatgagg cggatattgt gctggccaat gccgatctgc cctatgttta ccaggaactt
     2701 ttgccttcag gtcctcaggc caatcggata aaaaagctgg gatatacctg ctccgcaatc
     2761 gtttttcact gggggatgga cacggtttta ccggggattg agcagcataa tgtgtatgtt
     2821 tcagataatt acaggaacaa tattgaagag gttttcaacg gaagcgggtt tgcgcccgag
     2881 ccaagttttt acctgcattc cccggcacgg gctgataaat cggcagctcc ggaagggcac
     2941 gacagcattt cggtgattgt gccggtgggg catatgggga atgggaaatt tggggatttg
     3001 ggaattgatt gggaggttaa gaaggaggag gcgagggcgg cagtattgaa aagactggct
     3061 atggaaggat ttgcggattt tgagaaacat atcaaattcg agaaggttta caatcccgtc
     3121 atctggaaga atatgttcaa cctcagcaag ggtgccacct tcggcagttt aaatcacaac
     3181 ctgatgcaga tgggctattt tcgtcctcat aatcaacaca ataaatataa gaatcttttc
     3241 tttgccgggg gaagcaccca tccgggaaac ggtgtacctt tggcgctgat ttcggcaaga
     3301 ctgacagcgg agaaaataat tggagtgaac ggtgaacggt gaacggtgaa attgaataat
     3361 aactatgaat aatctggaaa ccattattgc tgaaaccttt agtaagattg attttcacaa
     3421 gatcaaagac caccccaaca ttctgattgc agcgcgtttc tgggaagatg aacgctacga
     3481 agcggcggca accatctacc gtttcatgcg ctacatcgat gatatgatcg acgaccggaa
     3541 ggcgctcgac gggatgttga gctgcatgga gaagaagatc tataccgacc aggtgaatgc
     3601 atggatcgac tgcctggggc ttgaccggac gaatgatccg tttttcggtg aggtaaccga
     3661 aacgatccgt cggttccgga tcccgttgca cttcttttac aactttgccc gttcgatggt
     3721 ctacgacatc aaccatgacg ggttccgcac attcgacgac tttcttgact acgccgaagg
     3781 ggcttccaac ggacccgcat cggtttttgt ccacctctgc tgtctggata aacaacaggg
     3841 agaatatgtg ccgatcaacc tttcaatctc ggatgtggcc aggccttgtg ctattttcag
     3901 ctaccttgtg catattgtgc gcgatttcca gaaagaccag ttcaacaacc tgaactattt
     3961 tgccctcgac ctgctggaaa agcacgggtt aactgccgga gatctcagag agatcgcccg
     4021 gggcagcgct attccggatg gtttcaggga actgatcagg gattataaag agcaggccgg
     4081 aaaatataag accgataccg aaaagatgat ccggtcgctg gaaggaaggc tggaccacag
     4141 gcatatgctc agcctgcgga tcatctacca tttgtacctg cagatttata accggatcga
     4201 tccggataac ggcacgttca cgatggccga gctgaatccg gggccggagg aggtgaaaga
     4261 agcggtggct gaatgtatca gggttaattt ttctgagaga accccgaagg tctgcccgga
     4321 gatacatcct cattgatttg ccggagttgg caacccgcaa gtatcaggca attaattcgt
     4381 gtatattctc ctttgatatc agtttgccgg tatttatttc atagttttcc gagaaagacc
     4441 tgggaaagcg ggcatttgat ttgcccctcc atttaaattc aaagatgttt attttaccgt
     4501 tggcttcttc aacaaggtca atttccgctc catcgtatgt tcgccagaaa taaagctgtg
     4561 caaatggctg atggatcagg ttatatttaa gtctttcgag gatgcagaag ttttcccaga
     4621 tcgctccggt atctgttctg gaatcgggat cattaaaatt ggttatcagc gcatttctaa
     4681 tacccagatc ataaaagtag aattttctgc tttttctgat ttcagttctt tgattcctgg
     4741 agaatgaagg aagggaaaat atgacaaagc ttttttcaag caggtcgata tacttttcaa
     4801 tagttttcga agatacagcc aatgtttttg atagttcatg aaatgatacc tgtgatccta
     4861 cctgcagagc cagcaactta agaagtttcc tcaataaaga aggatttctc agattttcat
     4921 gaatcaaaat gtctttaaac aggtaatccg aagccagatc aaacagtttc cttgttttaa
     4981 attctcctga tatatcaata attcccggat acgatccata tattggcatt agattcaaat
     5041 tttcataaat ccaaagccaa cctctggctc ctgataattc accgatagat aaaggaagaa
     5101 gtaagaattt aacatttctc ccggtcagtg gttcactaat agtatttgcc agctcaaaac
     5161 tgctggatcc ggtcgctatt attttctgaa caaaagaggg ctcatcatag accagtttca
     5221 gcagaggccc tatattccgg attttaagtg cttcatcaag ggcaataatt ttcttatccc
     5281 cgaagatcat ttttatcctc gataaatcag aggactcaag gatatcggca agatcaggcc
     5341 tttcacaatt caggattaaa gcatcaggat tttggtcgaa ca
//
LOCUS       DAOPNH010000243         5381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17693_length_5381_cov_5.246339, whole genome shotgun sequence.
ACCESSION   DAOPNH010000243 DAOPNH010000000
VERSION     DAOPNH010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17693_length_5381_cov_5.246339"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKZ74_11640"
     CDS             <1..1039
                     /locus_tag="PKZ74_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93449.1"
                     /translation="AQITITSEDMPVPGDTVRKSFTTFLQGFEYQVGGADQTWYFDEL
                     TVVSQQVDTFVEVEETPAIYQLFFNNQFIYPDYNSTVALKMAEFTAIPGLVLSDSYQF
                     IKVTDDEYREVGYGVNLEGVSLPIQLQEIDTIYRFPVEYGDIDSAHSLLEIEVPDLGY
                     LMVSKFRRNTVDGWGTLITPYGEFETLRMRTEITEYDSIYSDSLGIGMPLLRNIIEYK
                     WLANGYPEPLLVISEEPFLLTATYIDSVRTDFVSTPEIIKNDFQFSLYPNPANNHVSV
                     EYELAGDADVQISLLSVYGIELKRFLSAQQEQGLYKKVIYLKESGIPPGLYFLSLVID
                     QVPYTRKLIIN"
     gene            complement(1025..1540)
                     /gene="tnpA"
                     /locus_tag="PKZ74_11645"
     CDS             complement(1025..1540)
                     /gene="tnpA"
                     /locus_tag="PKZ74_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPM93450.1"
                     /translation="MAYTYSQINIHAVFTVLGRENLISPAFRGHLFGYISETMKGIGV
                     YPLAVNGTADHVHIFFELPPNISVSKALQEIKTSSSRWINNEGLVPGKFQWQKGFGAF
                     TNSRSQRDSVIKYILNQELHHAKNSFRDEYIELLQKYSVDFNPNNLFEFYVQDSENIL
                     PAETEGRLIND"
     gene            1805..4987
                     /locus_tag="PKZ74_11650"
     CDS             1805..4987
                     /locus_tag="PKZ74_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPM93451.1"
                     /translation="MLSDQRNFIVYKSSAGSGKTFTLVREYLKIALKNPDAFRQILAI
                     TFTNKAANEMKERVVRYLMALAEPTIYPEPTAEKYLLPELSAFLKMDEDTIRKRAADV
                     LARIIHHYAEFAISTIDSFTHRVVRTFAHDLRIPMNFEVELDSDMILVQAVDILLSMA
                     GSEEQLTRVLVEFVEKKAGDEKSWQIENDLARFGKLLLSESSFGAVSELRKLDLSGFL
                     EIRKELHRYTGAFESQIRELAMDCIRLIRDAGIAPDDFNYKDKGVYGYLEKLSQGRYD
                     KLKPGVHASNAFTSGEWLNRKASQQARDAFEMISDRLNYFAGTLTTLLDKDLQTYMLY
                     RLILGQIYSTALLSVLEKILNGICEDNNKLLISEFNRRISSIVRDQPAPFIYERLGEK
                     YHHYLIDEFQDTSVMQWHNLLPLVENSLASGRMNLVVGDAKQAIYRWRSGDVEQFEKL
                     PRLIKSYPDPILNQRENALVENYMEKSLAQNHRSAPVIVDFNNRFFTFVSELLPPGYG
                     QSYRNAVQTVTGKEKPGLVKIQQVTASEDEESSYDDRMMEAIYKQISELRDDNFEWKD
                     IAILCRKNEKAAKIAEYLVSKKIPVISSESLLVAKSAKVGFLLAWIRHLTDPEDEIQK
                     VHILAYLKTNGYLLNTFLEDFFNPKKDSPSSLFREVINGQEGFIGTEKLRTFEIYGLI
                     RYISHYYNLGADDDIYLRFFQDAVITFLGKERGGLPEFIDWWEEQQEKLSVIIPSGIN
                     AVRIMTIHKAKGLQFPVVIYPFADEKLRPGIDNIWVEIEDERLRPLRMACLPVAQALK
                     ETVYQQNFDDEMNRSLIDLVNVLYVVMTRPEERLYILMPPPPEKTEDANSVPKLLNRF
                     LKDGLQMTPSADVFLFGQRWKMPSILSSSDEKENNLAHDGRPVLNMLLKRRAPESWDI
                     ENPDRNREWGKLVHLALSLITVKDQTTRVLADLDSRGLIPHGKSDELKEQVLNVLNHP
                     AMAPFFTPMCEVRNEPEIIAPDGHLYRPDRVLINDGEAMLLDFKTGKKDESHKDQIAR
                     YGKLITEMGLQIRGGYLVYVQPVPDIIKVL"
     gene            5129..>5381
                     /locus_tag="PKZ74_11655"
     CDS             5129..>5381
                     /locus_tag="PKZ74_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93452.1"
                     /translation="MAALIIFPFAALTQDEARLLRFPAIHGDQVVFTYAGDLYTVAKS
                     GGMARKLTNHDGFEMFARFSPDGRQIAFTAQYDGNTEVYV"
BASE COUNT         1520 a         1192 c         1244 g         1425 t
ORIGIN      
        1 ggcccagatt actattacca gtgaagatat gccggtgccc ggcgatacgg tgcggaaaag
       61 ttttaccact tttcttcagg gttttgaata ccaggtcggg ggtgctgatc agacatggta
      121 tttcgatgag ttgaccgtcg tgtcgcagca ggtggataca tttgtggaag ttgaagaaac
      181 accggccata taccagttgt tcttcaataa ccagtttatc tatcccgatt acaattcaac
      241 cgttgccctg aagatggctg aattcacagc catacccgga ctcgttttgt cggactcgta
      301 tcagtttatc aaggttaccg acgatgaata ccgggaagta ggttatggcg taaaccttga
      361 aggagtctcg ctgccaattc agttgcagga gatcgatact atttaccggt ttcccgtcga
      421 gtatggcgat atcgattcag cccattcttt gcttgaaatt gaagtgcctg acctggggta
      481 tctgatggtg tcgaagttca ggcgaaatac ggtcgatggg tggggaacac tgataactcc
      541 ctacggcgaa tttgaaacat tgcggatgag aactgaaatt acggaatacg atagcattta
      601 cagcgattcc ctgggaatag gaatgccgct attaaggaat attatcgaat acaagtggct
      661 ggccaacggg tacccggaac cattactggt catcagcgag gaaccgtttt tgctgacagc
      721 aacctatatc gattcggtcc ggactgactt tgtgagcact cctgagatca taaagaatga
      781 ttttcagttt tctctatatc caaacccggc caataatcat gtttctgttg aatatgaact
      841 ggccggtgat gccgatgtac agatctcgct cctttcggtt tacggaattg aactaaaacg
      901 gttcttatca gcacaacagg agcaaggcct ttataaaaag gttatttacc tgaaagaatc
      961 gggcatccct ccaggactct actttttaag cttagtaatt gatcaggtgc cctatacccg
     1021 gaaattaatc attaattaaa cgcccttccg tttctgccgg caaaatattc tcgctgtcct
     1081 gaacataaaa ctcgaacagg ttattcggat tgaaatcaac gctatatttt tgcaacagtt
     1141 caatatactc gtccctgaag ctgttttttg catgatgtaa ttcctgattg agtatatatt
     1201 taataacact gtctctttgt gaccggctgt tggtaaatgc accgaatccc ttttgccatt
     1261 ggaatttgcc cggaacaagc ccctcattat tgatccacct tgaggaactg gtttttatct
     1321 cctggagagc tttagaaact gaaatattgg gtgggagttc aaaaaaaata tggacatgat
     1381 ccgctgtacc attgactgcc aggggataca ccccgattcc tttcatggtt tctgagatat
     1441 acccaaataa gtgacccctg aacgcaggcg atatcagatt ttccctgccc aatactgtaa
     1501 agacagcatg aatgttgatt tgtgaatagg tgtatgccat aatgattaaa ttttagggtt
     1561 aatgaatctt ttctattaat ccgaaaatcg accggaaaat acccggtgaa agcaaaataa
     1621 ttttttataa cactgaaata caaagattta ctaacgtcct tttgggacga tattacggta
     1681 tcaaacaaaa tcccccatcc catagagtcc catagggacg ccactatggt aaaaaaagaa
     1741 ttccccatcc tttagagtcc tgtagggacg ccactatggt aaaaaaaggt aattttatcc
     1801 catcatgtta tcagaccagc gaaatttcat tgtctataag tcatccgccg gatcggggaa
     1861 aacatttacc ctcgtgcgtg aatacctgaa aatcgcatta aagaaccccg atgctttccg
     1921 tcagattctg gccattacat tcaccaacaa agccgccaat gagatgaagg agcgcgtagt
     1981 ccgctacctg atggcgctgg ctgaaccaac catctacccc gagccaaccg cagaaaagta
     2041 cctcctcccc gaattatccg cattcctgaa gatggatgag gatacgatca ggaaaagggc
     2101 tgccgatgtg ctggcccgga tcattcatca ttacgccgag tttgccataa gcactatcga
     2161 tagttttact caccgtgtcg tgagaacgtt tgcccacgac cttcgcattc caatgaattt
     2221 tgaggtggaa ctcgactctg atatgattct tgttcaagcc gtagatatac tgcttagtat
     2281 ggctggttcc gaagagcaac tgacccgggt tctggtagaa tttgtcgaaa agaaagccgg
     2341 cgatgaaaaa agctggcaaa ttgaaaacga tctggccaga tttggcaaat tgctcctgtc
     2401 ggagtcaagt ttcggggcgg tcagcgagtt gcggaaactc gacctttccg gctttcttga
     2461 gatcaggaaa gaattgcacc ggtataccgg ggcttttgaa tctcaaatcc gtgaattggc
     2521 aatggattgt atacggttga tccgtgacgc cggaattgca ccggacgact tcaactataa
     2581 ggataaaggc gtgtatggtt acctggagaa attgtcgcag ggccggtacg acaagctaaa
     2641 accgggtgtg catgcctcca atgccttcac ttcaggtgaa tggctgaacc gcaaagcgtc
     2701 acaacaggcc cgcgatgcct ttgaaatgat cagcgaccgg cttaattatt ttgcaggtac
     2761 attgacaaca ctgctggata aggatttgca gacttacatg ctttaccggt tgattttagg
     2821 ccagatttat tcaacagcgc tgctttctgt tctggaaaaa atccttaacg ggatttgcga
     2881 agacaataat aaattgttga tcagtgagtt caatcgccgt atctcctcca ttgtcaggga
     2941 tcagccggct ccttttattt acgaaaggct gggcgaaaaa taccatcatt acctgataga
     3001 tgaatttcag gatacctccg taatgcaatg gcataacctg ctcccgctgg tggaaaactc
     3061 gctcgcttcc ggaagaatga acctggttgt cggcgatgca aagcaggcaa tttacaggtg
     3121 gcggtcgggg gatgtcgaac aatttgaaaa attaccccgt cttattaagt cttaccctga
     3181 tccaatactg aaccagcggg aaaacgccct cgtcgagaat tatatggaga aatcactggc
     3241 ccaaaaccac cggtcggctc ctgtaatcgt cgatttcaac aacaggtttt tcaccttcgt
     3301 ttccgaactg ctgccaccag gctatggcca gtcttaccgg aatgcggttc agactgttac
     3361 gggaaaagaa aagcccggac tggttaagat tcagcaggta accgcatcag aagacgaaga
     3421 aagctcctat gacgacagga tgatggaagc aatatataag caaatcagcg agttgcgcga
     3481 tgataatttc gaatggaaag atatcgcaat cctttgccgg aagaatgaaa aagcggcaaa
     3541 aattgccgaa taccttgtca gtaaaaaaat accggtcatt tcatccgaat ccttgctggt
     3601 cgctaaatcc gccaaagtcg gtttcctgtt ggcctggatc cgccatttaa ccgatccgga
     3661 agatgaaatc cagaaagttc atatccttgc ctatttaaaa accaatgggt atcttctgaa
     3721 tactttcctt gaagattttt ttaatccgaa gaaagattca ccttcatccc tgtttcggga
     3781 agtgattaat ggtcaggaag gattcatcgg aacagagaaa ttaaggactt ttgagatata
     3841 cggactaatc cggtatatat cccattatta taacctggga gccgatgatg atatttatct
     3901 ccggttcttc caggatgccg tcattacatt tctcggaaaa gaaaggggag ggttgccgga
     3961 gtttatcgac tggtgggaag aacagcagga aaaactgtcg gtcattatcc cttcgggaat
     4021 taatgcggtc aggatcatga caatacataa agcaaagggg ctacagttcc cggtagtcat
     4081 ttaccctttt gcggatgaaa aactaaggcc tggtattgat aatatctggg tcgaaataga
     4141 agatgagcgg cttcggccac tccggatggc ctgtctcccg gtcgcgcagg cccttaagga
     4201 aaccgtttac cagcaaaatt tcgatgatga aatgaaccga tccctcatcg atctggttaa
     4261 cgttctttat gttgtcatga caaggccgga agaaaggttg tatatcctta tgccacctcc
     4321 gccggagaaa accgaggatg ctaattctgt cccgaaatta ttgaaccggt tcctgaaaga
     4381 cgggcttcaa atgacaccct cagcggatgt ttttctgttc ggacaaaggt ggaaaatgcc
     4441 gtccatactg tcttccagcg atgaaaagga aaataatctg gcccatgatg gaagacctgt
     4501 attgaacatg ctgctgaaaa ggcgtgcccc ggaatcgtgg gatatcgaga atccggaccg
     4561 gaatcgggaa tggggaaaac tggtacacct tgctttatca ctcattacag ttaaagacca
     4621 aaccaccagg gtgttagcag acctggattc gcggggtcta atcccacacg gtaaatcaga
     4681 cgaactaaag gaacaggtgc ttaatgttct caatcatcca gcaatggccc catttttcac
     4741 gcccatgtgt gaggtcagga atgaaccgga aattatcgca cctgacgggc atctgtatcg
     4801 tcctgaccgt gtattgatca acgatggtga agctatgctg ctggatttta aaacagggaa
     4861 aaaagatgaa tcacataaag accagattgc caggtacggc aagctgatca cagagatggg
     4921 tttacagata cggggtggct acctggtcta tgtccagccc gtgcccgata tcataaaagt
     4981 tttgtaagac aattccgccc actcgtcaat cggaaattac caacggtgct tgttttgcgg
     5041 caaaagggca ttttaccggg tttggtttaa tctttcatat taatttagcc ggtaaaaaca
     5101 taacagtatg agaaatttga tattgacaat ggctgccctg ataattttcc cttttgcagc
     5161 ccttacacag gatgaagcca gattactgag attccccgca attcatggcg accaggttgt
     5221 ttttacctat gccggagacc tttatacggt tgcaaaatca ggtggaatgg cccgtaaact
     5281 gactaaccat gacggttttg aaatgtttgc ccggttctcg cctgatggca gacaaattgc
     5341 ctttacagcc cagtacgatg gcaataccga agtttatgtg a
//
LOCUS       DAOPNH010000244         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_17997_length_5315_cov_5.194487, whole genome shotgun sequence.
ACCESSION   DAOPNH010000244 DAOPNH010000000
VERSION     DAOPNH010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17997_length_5315_cov_5.194487"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKZ74_11660"
     CDS             complement(<1..987)
                     /locus_tag="PKZ74_11660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM93453.1"
                     /translation="MNISIIIPVYNNLQLSINCINSIAELSFYKNDLEVIIVDDGSDD
                     GTAIYFTGFEKENNWLIYHRNSSNLKFARSCNEGAKVARGQFIVFLNNDTLVTINWDK
                     ILLDTIENDRDTWMVGAKLLYPNSMIQHAGVYLPELTGVSFGHVYNGFPSDFPPANIG
                     KEVQCVTAACMIMRREDFLAIGGFDTGFINGSEDIDLCLRIVDKGKKIVYQPQCEIIH
                     LESKSEGRFDQSSQNSARLFERWKGKVRSDLVDLMEQDLKTCENNGQLILISREKINL
                     QAPVMKFSFAIPSRDLLVGNGLVIGLGCIATEKSIIRLSYSTRSGYSDDKILN"
     gene            complement(1009..3237)
                     /locus_tag="PKZ74_11665"
     CDS             complement(1009..3237)
                     /locus_tag="PKZ74_11665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM93454.1"
                     /translation="MESSILNQLNIIRSGERKKVLDQALALINNKGKDFEKPPVELIN
                     QLNRLSADIGMNNDFRRALENQDKLSNLLIKLYEGADPDLVQILPSTNDPAEFHRKMK
                     AYLPEQPVFKRLDPFFFLNKDVVVNKEMISVIVLNENGAPYLDDFFNSFAKFCSFNDY
                     RITMFDRASADNSIEIASSWCDRIPLEIIRFDERLTASYLYNTAVDNSSSQYILFVAN
                     SISFNQDILQKFLETIQKDESTGITGASLLLKDISGKSQEMELFGSILFRIEDIPGVP
                     DIPFPTINTRLLLKTGLDKHLKHYRKEGAVFVPPGLSMKGHKPFPASHTRKTGDEPVM
                     AVTGHALFCRRQDLIASGGFDLNFTDGFEEADLCLKFARRLKKKTILSHEIRVEMNNE
                     DSLKTMGGKFAIVNRYNLGTLINNHGGYLKRKYPADNTEPENQHIRIAIKTPAFDDER
                     TLMWGDYHFASSLKNAFVRKGYQARVDLHNFWYDNGYMTDDVVIVLRGTKRYHTRGSQ
                     VNILWNISHPEQVTEEEYKDYDYVFSASTFFADELKKKYGIMAENLLQCTDAGLFYPD
                     KENISEEEKNDVLFVGNSREVMRKSVAYSLEKGIPLAVYGSRWEQFLPAKMIKGNYLK
                     NEALRKYYSNCNLLLNDHWRDMADHGFISNRIFDALACGAIVLTDRVKGIEDVISKGL
                     YYYNTADELEHQIKWLKENGDQAKRLALENAEIIAHNHSFDQRAGRILEIALRIHNQK
                     TE"
     gene            3568..>5315
                     /locus_tag="PKZ74_11670"
     CDS             3568..>5315
                     /locus_tag="PKZ74_11670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM93455.1"
                     /translation="MTDGFIDRAYPELNEMAKSHEDDLSRKEAAWELALYHSVRKTKE
                     DAKGCMKFLSSSTDENADMSRTIQAAIIRSECHNLLGEKHKAAEILITAIEKCPSPEL
                     LLAYANLAGDLKTKVECFNKAFMMSGLGPGLKESYSLLPYFFRIRSLEVIPEGIHADV
                     ARPLVSIIIPTYNCSDTMGYVMDSVLSQTWKNIEVIVADDCSTDQTAGIVRGYLQKDK
                     RVKFIRTEANSGLFVAMNKALQIAKGEFVTCQDQCEWAHPEKISIQAGHLVGNPDYTA
                     NISRRIKVNSNLEPLRAEHSGLFIQNNIRSLMFRRQRVMTSLGFWDSVRAGADEEMLF
                     RLRRKFSDKRICYLETGPLAFLLEETSSFAKKRNPDVSPGALREYMESFKSFLAKNDS
                     PSYEFPLKARRYPVPEPFWPVAEKKTNGRRHFDVLLASEFRLPGGTTTSNVEQIKAQR
                     AAGLKTGLVHLKRYDINTSEPIRDKIRAEIDGDLVQMVVPGEKVSCDLLVVRHPPVLK
                     DFQLFVPDIQAGHLLVVINTSPLREYSIQGERVYDFRICKENLVKYFGKEGIWCPIGP
                     LVRDAVMQEDNILNII"
BASE COUNT         1453 a         1173 c         1170 g         1519 t
ORIGIN      
        1 attgagaatt ttatcgtctg aataccctga ccgggtgctg tagctgagcc tgataatgga
       61 tttttcagtg gcaatgcaac ccagtccgat cacaagccca tttccaacta aaaggtcacg
      121 ggaaggaatc gcaaaagaaa atttcatgac aggggcctga aggtttattt tttcccggct
      181 gatcagaata agttgcccat tgttctcaca ggttttcaaa tcctgttcca taagatcaac
      241 cagatcagac cgtaccttgc ctttccatct ttcaaagagc ctggccgagt tttgtgagga
      301 ctggtcgaac cgtccttcac ttttggattc caggtgaatt atttcacatt gcggctggta
      361 aacaatcttc tttcctttat ccactatcct caggcacagg tcaatatctt cggagccatt
      421 aatgaatcct gtgtcaaatc cacctattgc aagaaagtct tcccgccgca tgatcataca
      481 tgctgccgta acacattgca cttctttgcc aatattggca ggtgggaaat cagacggaaa
      541 tccgttgtaa acgtgaccga agctcactcc cgtcaattca ggcaaataaa caccggcatg
      601 ctgaatcata ctattcggat agagcagttt tgccccaacc atccaggtat ccctgtcatt
      661 ttcaatcgta tcaaggagaa ttttatccca gtttattgtc acaagggtat cattattcaa
      721 aaagacaata aattgacccc tggccacctt tgcgccttca ttgcatgatc gggcgaattt
      781 caggttggaa gagttcctgt ggtaaatcaa ccagttattt tctttttcaa atccggtaaa
      841 atagattgct gtgccatcat cggaaccatc gtcgacgatg atcacctcca ggtcattttt
      901 gtagaaagat agttccgcaa tactgtttat gcaattgatt gacagctgca ggttattata
      961 aacagggatt atgattgaga tattcatgat gaaattcttg actgcttgtc attctgtttt
     1021 ctggttgtgg attctcaagg ctatttccag tatccttcct gcacgctggt cgaatgaatg
     1081 attatgggcg attatttcag cattttccaa tgccaggcgc tttgcctgat caccgttttc
     1141 ctttaaccat ttaatctggt gctcaagttc atccgcagta ttataataat aaagtccttt
     1201 gctgatgaca tcttcgattc ctttcacccg gtcggtcaga acgatggcgc cgcaagccag
     1261 ggcatcgaag atccggtttg agataaaacc atgatcggcc atatcccgcc aatgatcatt
     1321 caataaaagg ttgcagttcg aataatattt ccgcaatgct tcatttttta aataattgcc
     1381 tttgatcatt tttgctggca ggaattgttc ccatctcgat ccatacacag caagcgggat
     1441 tcctttttcg agtgagtatg caaccgattt tctcatgact tcccttgaat ttccgacaaa
     1501 caaaacatca tttttctctt cctccgatat gttttccttg tcgggataaa acagccctgc
     1561 atcggtacac tggagaagat tttccgccat tatcccgtac ttctttttca attcatccgc
     1621 gaaaaaggtt gaagcggaaa acacgtaatc atagtctttg tactcttctt cggtgacctg
     1681 ctccggatgg ctgatattcc ataatatatt gacctgggag ccacgcgtat ggtatctttt
     1741 ggtccctctt aaaactatta ccacatcgtc ggtcatgtat ccgttgtcgt accagaagtt
     1801 atgcagatcg actcgggcct ggtagccctt ccggacaaag gcatttttaa gcgaactggc
     1861 aaagtgataa tcgccccaca ttaaggtccg ttcatcatca aaagcgggtg tttttatcgc
     1921 aatgcgtatg tgttgatttt ccggctcagt gttatctgct ggatattttc ttttcaggta
     1981 cccgccatgg ttattgatca atgtgccgag gttatacctg ttcacaattg caaattttcc
     2041 tcccatggtt ttcaatgaat cctcattgtt catttcaacc cggatttcat gggagagaat
     2101 tgtcttcttt tttagcctcc tggcaaattt caggcacagg tctgcctctt caaacccatc
     2161 cgtaaaattc aggtcgaaac cgcctgatgc gatcagatcc tgccgcctgc agaacagggc
     2221 atggcctgtc acagccatca ccggttcatc tcctgttttc cgagtgtgac tcgccggaaa
     2281 aggtttatga cctttcatcg acaaaccggg tggaacaaaa accgcccctt ctttcctgta
     2341 atgtttcagg tgcttatcaa ggccggtttt taagagcaaa cgggtattga tcgttggaaa
     2401 gggaatatca ggcacaccgg gaatatcttc aatgcggaac aggatactgc cgaacagttc
     2461 catttcctgg gattttccgg agatatcttt caataacaga ctggcgccgg taatgccggt
     2521 cgattcatct ttttgtattg tttccaggaa tttttggaga atatcctgat tgaatgagat
     2581 ggagttagca acgaaaagaa tgtactgcga tgaactgttg tcaacagcgg tattataaag
     2641 ataagaagcc gttaatcgct catcaaaccg gatgatctcc aatgggatcc tgtcgcacca
     2701 ggaagaggca atttcaatgc tgttatctgc cgaagccctg tcgaacatgg tgatccggta
     2761 atcattaaag gaacagaatt ttgcgaatga attaaaaaaa tcatcaaggt atggcgcccc
     2821 gttttcattg aggacaatta cgctgatcat ttccttgtta acgacgacat ctttattcag
     2881 gaagaagaaa ggatccaatc ttttaaaaac cggttgttca gggagataag ccttcatttt
     2941 cctgtgaaat tctgcaggat catttgtcga tggcagaatc tgaaccaggt ccggatcggc
     3001 gccttcatat aattttatca gcagattgct cagtttatcc tggttctcca gtgcacgcct
     3061 gaaatcattg ttcattccaa tatccgccga cagcctgttc agttggttta tcagctcaac
     3121 cggcggcttt tcaaagtcct tacctttgtt atttatcagc gcaagagcct ggtcaagaac
     3181 ttttttccgc tccccggacc tgatgatatt taactgattt aaaatagaag actccataac
     3241 taattacccc gtacgctgtt tatctgtctg ttataaaaac atcgaaggta aggaaaccag
     3301 aattaaaaat ttgtaccttg ccatatatta tactcattat atttgatcga aatggtgaat
     3361 ctaaaactgc ttttgcgatc actaaaatac caatttccgg gaaatttaag gctgataaaa
     3421 ctgccatcct ttcccgatat atccgctaac cggttgccag gacccgacaa agaaccgaaa
     3481 gtaatgaatt cggatgagtt tagtcctgac gatcttgaaa taattaagcg gaataaatgg
     3541 cgaattgcaa atattgccag caaattaatg accgatggtt ttatagatag agcctatcct
     3601 gaactgaatg aaatggcaaa atcccatgag gatgatctct cgcgcaagga agctgcatgg
     3661 gaacttgctt tatatcattc cgttcgtaaa acgaaggagg atgcaaaagg atgcatgaag
     3721 ttcttatcat catccactga tgaaaatgcg gatatgtcca ggaccatcca ggcagccatc
     3781 atacggtcag aatgccataa tttactggga gaaaagcata aagccgcaga aatactaatt
     3841 acggcaattg agaaatgtcc atcgccggaa ctgttgctgg catacgcaaa tcttgccgga
     3901 gatctgaaaa caaaggttga atgcttcaac aaggcattta tgatgtcagg acttggccct
     3961 ggccttaaag agagttattc acttttgcct tatttcttta ggatcagatc gctggaagtc
     4021 attcctgaag gtattcatgc cgatgttgcc aggccgcttg tttccataat cattcctact
     4081 tataattgca gtgacacaat gggttacgtc atggattcgg tgttgagcca gacctggaaa
     4141 aacatcgaag tgatcgtggc tgatgactgc agcactgatc aaactgccgg tattgtcagg
     4201 gggtatttgc aaaaagataa gcgggttaag tttattcgga cagaagctaa cagcgggctg
     4261 tttgtagcca tgaacaaggc tttgcaaata gcaaaaggag aatttgtgac ttgccaggat
     4321 caatgtgaat gggcgcatcc ggaaaagatt tccattcagg cgggccacct tgttgggaat
     4381 cccgattata ctgcaaatat ttcccggcgg ataaaggtga acagtaatct tgagccgctc
     4441 agggccgaac attcagggct tttcattcag aataatatcc gatccttgat gttcagaagg
     4501 caaagagtaa tgacgagcct tggattctgg gattctgtca gggccggcgc cgacgaggag
     4561 atgcttttcc ggctcaggcg aaaattcagt gataaaagga tatgttacct tgaaaccggg
     4621 ccattggcct tcctgttaga agaaaccagc tcatttgcaa agaaaaggaa cccggacgtt
     4681 tcgcctggcg ctttgagaga atacatggaa tcatttaaat catttttggc gaaaaatgat
     4741 tctccgtcgt atgaattccc gcttaaagcg aggagatacc cggttccgga gcccttctgg
     4801 ccggtggctg agaaaaagac aaacggcagg cggcattttg atgtcttact agcgtcagag
     4861 ttcaggttgc ccggcggtac gaccacgtcg aacgtagagc agatcaaggc tcaaagggct
     4921 gcaggattaa agacgggcct ggttcacctt aaacggtacg acatcaatac atcggagccc
     4981 atacgtgaca agatcagggc tgaaattgac ggagaccttg ttcagatggt ggttcccggg
     5041 gagaaagtat cgtgtgacct cctggtggtg cgacatccac cggttttaaa agatttccag
     5101 ttatttgtgc ctgatatcca ggccgggcac ctgctggttg tcatcaatac ttcgccatta
     5161 cgggaatata gcatacaggg agaaagagtt tatgatttcc ggatttgtaa agaaaacctc
     5221 gttaaatact ttggcaagga aggcatctgg tgcccgattg gcccactggt cagagatgct
     5281 gtcatgcagg aggataacat cctgaatatt attaa
//
LOCUS       DAOPNH010000245         5304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18043_length_5304_cov_5.613450, whole genome shotgun sequence.
ACCESSION   DAOPNH010000245 DAOPNH010000000
VERSION     DAOPNH010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18043_length_5304_cov_5.613450"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..635)
                     /locus_tag="PKZ74_11675"
     CDS             complement(294..635)
                     /locus_tag="PKZ74_11675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPM93456.1"
                     /translation="MIARQYEIWIADLNPRIGTEPGKVRPVVIVQTDLLNKHHPSTLI
                     CPVTTSVQPESEILRVHLAKGSSGLDEACDVMMDQVRAIDNARLLKKIGKLPSEAILK
                     VKENLSIVLDL"
     gene            complement(616..855)
                     /locus_tag="PKZ74_11680"
     CDS             complement(616..855)
                     /locus_tag="PKZ74_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93457.1"
                     /translation="MKTLSLKLDDNVFRETEQVTGYLKKSRNRYINEALDHFNKVHQR
                     KILADRLEMESKLVAKESADVMNEFEAFEDDSQAI"
     gene            complement(1055..1873)
                     /locus_tag="PKZ74_11685"
     CDS             complement(1055..1873)
                     /locus_tag="PKZ74_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPM93458.1"
                     /translation="MMRLLQIELRKILPYKMFWVMCGIYLVSLLFFSWGLPSLIDYFT
                     MKSNVPEIKLLTKFLYNFPDIWQNLSWAASLRFFIKIFIGIIMIILVTNEYSYLTIRH
                     NVFNGLSRTDFLMSKVYVAILISLAATLLILLTGITLGLFYSSTISVQAFFGKMAFLA
                     GYFIELLAFLLFSLMVGILIKRTGFAIGVLFVYPILELIIQQKVPESVQPFLPLNAIN
                     HILRTPNTSLIQFSSPEFDIELQTALHAQDILVALAYAVLFVLVSLWVIRKRDL"
     gene            complement(1870..2766)
                     /locus_tag="PKZ74_11690"
     CDS             complement(1870..2766)
                     /locus_tag="PKZ74_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM93459.1"
                     /translation="MKDIVLSINGLTKRYGSITAVSRLDLEIERGSVFGILGPNGSGK
                     TTTLGVLLDVTRPDSGSFSWFGGLPEKTQRKRIGAILEVPLFYPYLSGKQNLELVAKI
                     KDLPGDDVEEALQTVGLYDRMHSKFRTYSLGMKQRLALASTLIGKPEVLILDEPTNGL
                     DPRGIAEIRNIILNVAAQGVTIILASHILDEVQKTCSHVAVLEKGKKLFSGPVAQVLN
                     EAHWVELAAENMAGLQLALQEAEFIENLQEEGDRYIIKLKSDLSQADLNRYFFGKGIV
                     LSHLAIRKKSLEKYFLELLDKS"
     gene            2890..3402
                     /locus_tag="PKZ74_11695"
     CDS             2890..3402
                     /locus_tag="PKZ74_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM93460.1"
                     /translation="MTIGEKSVVSMTYTLREESSAGQVIQKVTEDRPFVYLFGVAGLL
                     PAFKANLQGKTSGEEFAFTLSNEDAYGLPSDENVLSLDKKIFEIDGVFDEANIKVGEV
                     VPMEDENGYPLSGKILEIGTDSVLVDFNHPLAGMNLFFEGKILEVREATDEEMAHGHV
                     HGPDGHHHHH"
     gene            complement(3403..4110)
                     /locus_tag="PKZ74_11700"
     CDS             complement(3403..4110)
                     /locus_tag="PKZ74_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPM93461.1"
                     /translation="MKGKLYLLPSTLGDGSPLSVIPPVVPETIAKLRFFIVEDIRSAR
                     RFLKKTNPAIVIDELTFLVLNEHTDPGEIPALLEPMINGNDAGQLSEAGLPCVADPGS
                     LLVGMAHESGITVVPLPGPSSIFLALMASGFNGQNFAFHGYLPIDKKERIQKIREIES
                     AVYTKDQTQVFIETPYRNKQMAEALNQACRPDTKICIAVDLTLPEEKIHHTTAKRLNP
                     DHPSGLNKKPAVFLMYH"
     gene            complement(4107..5066)
                     /locus_tag="PKZ74_11705"
     CDS             complement(4107..5066)
                     /locus_tag="PKZ74_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPM93462.1"
                     /translation="MIPDLQTIQAAAIRIKHYIHRTPVLTSRCINEMTGAEIWFKCEN
                     LQKVGAFKSRGACNAVFSLSDEEVTHGVGTHSSGNHAQALARAASIRGCKAYIVMPET
                     SPKVKSDAVRGYGGEITFCKPTLEARESTLAEIIHRTGATEIHPYNDYRIIAGQATAA
                     MELIEETGPPDMIMCPVGGGGLLSGTALATSYLSPSTKVIAAEPAGADDAFRSFTSGK
                     FVPSVNPKTICDGLLTSLGSRTFPIILEKVHRIITVQEETIIRAMMLTWERMKIIIEP
                     SSAVPLAAILENPSVFAGKRVGIIISGGNVSLDKLPWIKSSGS"
     gene            complement(5070..>5304)
                     /locus_tag="PKZ74_11710"
     CDS             complement(5070..>5304)
                     /locus_tag="PKZ74_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93463.1"
                     /translation="DRNNYNDVMGVARDSKDEKKVDYMLNLVYPGENRPVPDPWYGGK
                     EGFEKVYQMLDEACENLINELSHPLPPLQPLQP"
BASE COUNT         1337 a         1268 c         1315 g         1384 t
ORIGIN      
        1 ccttcggccg ctctttgcct tcaaaaagac ctttgctaat tgttatcttt ttcatgatgt
       61 ttgttttttc accacatagg cacataagga cacatagatt tcacataggc ttgtcctttt
      121 gagtttctct actatgtgtt tctatgtgcc ctaatgtgcc tatgtggtga aataatcgat
      181 ctctccatat agcttaatcc ggaaaacaga aaaaataccc ggtaggaatg gaaaaaagtg
      241 aaaaacgaat agtgaatagt gaataatgaa aaggaagaga ttagtttatc ttattataaa
      301 tcaagcacaa ttgatagatt ttctttcact tttagaatcg cttctgaagg cagctttcca
      361 atttttttta ataaccttgc attgtcaatc gccctaacct ggtccatcat cacatcacag
      421 gcttcgtcaa gtccactact gcctttggct aaatgaaccc gcaatatctc gctctccggc
      481 tgaacactcg tagtaaccgg acaaataagc gttgaaggat gatgcttatt cagtaaatca
      541 gtctgaacaa taacaacagg acgcaccttt cccggctcgg ttccaatccg cggattcaga
      601 tcggcaatcc agatctcata ttgcctggct atcatcttca aacgcctcaa attcattcat
      661 cacatctgcc gattcctttg cgacaagctt cgattccatt tccagccggt cagcaagtat
      721 tttccgctga tggaccttgt tgaaatgatc caaagcctcg ttaatataac ggttacgcga
      781 ttttttcaaa tatcctgtta cctgctcggt ctcccggaat acattgtcat ctaattttaa
      841 agaaagtgtt ttcatgatat gttatcagta tattgcaaaa gtatatactt tccggaattg
      901 aaccaatttc ttttacaaca taaactacac aaaagctgtt acgcaaagtt gcgcaaagtg
      961 aaagcaaagg ttcgcaaaga agtgttaagg gcagaattct aagcagttaa taaaataacc
     1021 cgaatttata attcaacatt caacattcaa catttcaaag gtcgcgtttc cgtatgaccc
     1081 acaacgaaac cagcacgaat aaaacggcat atgccagggc aacaagtata tcctgtgcat
     1141 gcaatgccgt ctgcagctca atgtcgaact cgggagaact gaactggatc agcgaggtgt
     1201 tgggtgtgcg gagaatgtga ttgatggcat tcagcggcag gaacggctgc acgctctccg
     1261 gcaccttctg ctggatgatg agctccagga tcggataaac gaaaagcaca ccgatggcaa
     1321 aaccggtccg cttgatcaaa atgccgacca tcagcgaaaa cagtaagaat gccagcaatt
     1381 caatgaaata gccggctaaa aatgccatct tgccaaaaaa agcctgaacg cttatcgtcg
     1441 aagaataaaa caatcctaac gtgatgccgg tgagtaaaat cagcaaggtg gccgccaaac
     1501 tgatcagaat ggccacatat accttcgaca tcaggaaatc ggtgcggctc agaccattga
     1561 aaacattatg ccggatcgta aggtaggaat attcattggt gaccaggatg atcatgatga
     1621 tcccgataaa aatcttaatg aagaaccgga gcgaggccgc ccacgacagg ttctgccaga
     1681 tatcagggaa attatacagg aatttggtca ggagcttgat ctcggggaca ttcgatttca
     1741 tggtgaagta gtcgatcagg ctcggcagtc cccaggaaaa gaataataac gacaccaggt
     1801 aaatcccgca cattacccag aacatcttgt agggcaatat tttccgtaat tcgatctgaa
     1861 gaagtctcat catgatttgt ccaataattc gagaaaatat ttttcaagag attttttacg
     1921 aatcgccagg tgcgataaca cgattccttt gccgaagaaa taccggttca ggtcggcttg
     1981 tgagaggtcg gatttcagct tgatgatgta ccggtcgcct tcttcctgca ggttttcgat
     2041 gaattctgcc tcttgcagcg ccaattgcag accggccata ttttccgcag ccagttccac
     2101 ccagtgggct tcattcagca cctgcgccac aggtcctgag aacaactttt tgcctttctc
     2161 caggaccgcg acatggctgc aggttttctg cacctcgtcg aggatgtggc tggccaggat
     2221 gatcgttaca ccttgtgccg ctacattcag gataatgttc ctgatctcgg ctattcccct
     2281 gggatcaagc ccgttggtgg gttcatccag gatcagtact tcgggtttgc cgatcagtgt
     2341 cgaggccagc gccagtcgtt gcttcatccc caggctgtaa gtccggaact tgctgtgcat
     2401 ccggtcgtac agccctaccg tctgaagcgc ttcctccaca tcgtcgcccg gaaggtcttt
     2461 gatcttcgcc acgagttcca ggttttgctt tccgctcagg taaggataaa aaagagggac
     2521 ttccaggatg gcgccgatcc gctttcgctg tgttttttct ggcagtcccc cgaaccagga
     2581 gaacgagccc gagtcgggcc gcgtgacatc gagaagcacg ccgagggttg ttgtcttacc
     2641 gcttccgttg ggcccgagga tgccgaaaac tgatccccgc tcaatttcca ggtccagtcg
     2701 gctgaccgcg gtgatcgatc cgtatcgttt ggtcaggcca ttgatggaaa ggacgatatc
     2761 tttcaaatgt tttgatttgc gttaaacaaa tataaaacga tactgcattg atttcattac
     2821 aacggaacgg aataattttc cacccaaact ttgttatctt tgccgccata acttaaaatt
     2881 ccaaacggta tgacaattgg tgaaaaatcg gtcgtttcga tgacctacac attgcgtgaa
     2941 gaaagctcag cggggcaggt gattcagaaa gttacggagg acaggccatt tgtctacctg
     3001 ttcggcgtgg ccggacttct tccggctttt aaagccaacc tgcagggaaa aacctccggt
     3061 gaagaatttg cttttacgtt aagcaatgaa gatgcttacg ggcttccttc ggatgaaaat
     3121 gtcctcagcc tggacaagaa gatatttgag attgatggtg tgttcgacga ggcaaacatt
     3181 aaagtagggg aggtggtacc gatggaagat gagaacggat acccgctgtc gggtaagatc
     3241 ctcgagatcg gcaccgattc tgtattagtc gatttcaacc atccgctcgc cggcatgaac
     3301 ctcttttttg aagggaagat cctggaagta cgcgaagcta ccgatgaaga gatggcccat
     3361 ggccatgtgc acggtcccga cgggcaccat caccaccact gatcagtgat acatcaggaa
     3421 tacggccggt ttcttgttca gaccggacgg atgatccggg tttagccgct tcgcggtcgt
     3481 gtgatgtatt ttttcttcgg gaagggtcag atccaccgca atacaaattt tcgtatcggg
     3541 cctgcaagcc tggtttaaag cctcagccat ctgtttgtta cgataaggtg tttcgataaa
     3601 gacctgggtc tggtcttttg tgtaaacagc cgactctatt tccctgatct tctgaatcct
     3661 ctctttttta tcgatgggaa gataaccgtg aaaagcgaaa ttctgcccgt taaatcccga
     3721 ggccatcagg gccaggaaga tcgacgacgg accgggcagc gggactactg tgatcccgga
     3781 ttcatgggcc attccgacga gcaaagagcc gggatcggcc acgcagggaa ggcccgcttc
     3841 cgataactgg ccggcgtcat ttccattgat catgggctcg agcagggcag ggatttctcc
     3901 gggatcggta tgctcattca ggacaagaaa agtcagttca tcgatgacga ttgccggatt
     3961 ggtctttttc agaaagcggc gggcgctccg gatatcctcc acgataaaaa acctaagttt
     4021 ggcgatggtt tccggcacaa ccggggggat caccgacagg ggcgaaccgt cgccaagggt
     4081 agagggaagc aggtaaagtt ttcctttcat gagcctgagc ttttgatcca gggcagttta
     4141 tcgaggctga cattgccgcc cgaaataatg atcccgaccc ttttcccggc aaaaacagac
     4201 gggttttcca aaatggcggc cagtggcaca gccgaggagg gctcgatgat gatcttcatc
     4261 ctctcccagg tgagcatcat ggccctgata attgtttctt cctgcacggt gattatccgg
     4321 tggacttttt ccaggatgat cggaaaggtc ctggatccca gagaggtcag cagcccgtcg
     4381 caaatggttt tcgggttcac cgaagggacg aatttcccgc tggtaaagga acggaaggca
     4441 tcgtcggcgc cggcgggttc tgctgcaatg actttagtgg aagggctgag gtaagatgtg
     4501 gccagcgcgg tgccactgag caggcctcca cctccgaccg ggcacatgat catatccggc
     4561 gggccggtct cttcgataag ttccatcgca gccgtagcct gtccggcgat gatcctgtaa
     4621 tcgttataag gatggatctc tgtggctccc gtccggtgaa tgatctcagc cagtgtggat
     4681 tcgcgcgctt ccagggtagg tttgcagaag gtaatttcgc cgccgtagcc tcttaccgcg
     4741 tcacttttta ctttcgggga ggtttcgggc atgacaatat aagctttgca tcccctgatc
     4801 gaggctgcac gggccagcgc ctgggcgtgg ttgcccgacg aatgggtgcc tactccgtga
     4861 gtaacctctt cgtcgctaag cgaaaatacg gcattacaag cgcctcgtga tttgaatgcg
     4921 ccgactttct ggagattttc gcacttgaac catatctcag ctccggtcat ttcattgata
     4981 catcgggatg tgagcaccgg tgtgcgatgg atgtagtgtt tgatccttat agctgccgct
     5041 tgtatggttt gaaggtcggg aatcattgtt cagggttgta agggttgtaa gggtggtaag
     5101 gggtgtgaaa gttcattgat taagttttcg caggcttcat cgagcatttg gtagactttt
     5161 tcaaagcctt ccttgccgcc gtaccaggga tcggggacag ggcggttttc tccgggatag
     5221 accaggttca gcatatagtc gacctttttt tcgtctttgc tgtcacgggc cacacccatt
     5281 acatcattat agttattacg gtcc
//
LOCUS       DAOPNH010000246         5288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18127_length_5288_cov_4.344735, whole genome shotgun sequence.
ACCESSION   DAOPNH010000246 DAOPNH010000000
VERSION     DAOPNH010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18127_length_5288_cov_4.344735"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2512)
                     /locus_tag="PKZ74_11715"
     CDS             complement(<1..2512)
                     /locus_tag="PKZ74_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93464.1"
                     /translation="MNLRNIKIRILLIAGLGLMLLMAFITGIIAYNQSRLIHEQTELI
                     YNHPFKVRRAIGHLTTDIVSIQRDVRNLMLFDDHDVVSENLIQIMHAREDAARQIEIL
                     ELHYLGPKSDVDSLKEAFSRWNATRDEKIGIILNTDSGRTQNSLQVLNALNRDVGVVF
                     HFIGDIDTFAKNKADQLYEDSQILSRKLNIRLVIVIIISMLVSIIIFNILIRNIRQPL
                     DELSSATQQFREGNYRIRSQYHSSNEFGLLSGAFNDMADYLESIIILNQKTEQIERLM
                     LAENDPRLFFRTIMTRLCESTGAQIATVYLYNKPSQTFDLFESIGLEEPARKSFPAEV
                     LEGDIGLAVTTRKVQHITHIPGDTPMRFPAAGGMITPREIITAPVAVGDDVIAVISLA
                     TIKNFSKQDSELVKEILAALSARIEGILSYQKVRELSAKLEIQNQELEMQKNELMTQS
                     TILGRQNTELETQKQHLAEASRLKTNFLSNMSHELRTPLNSVIALTGVLNRRLEGKIP
                     AEEHSYLEVIERNGKNLLALINDILDISRIESGRVEMEFTDFNLTELISGIVNMIAPQ
                     ASQKNITIDFAPEKESMVIHSDAGKIYHILQNIIGNAVKFTDKGGVQISTRHLDDKVG
                     ITIKDTGIGIAEEHLPYIFDEFRQGDSSTSKRYGGTGLGLALAKKYADLINGKINVQS
                     QVGKGSDFTLSIPVHLSTGEESGPFDYSAGNPYHLKKTAGTPDSPELAGSIMLIEDSE
                     PAIIQIQDVLSQQGFNVIVARDGDEALERMNSFTPDAIILDLMMPGKSGFDVLQAVRE
                     SEKTAYVPVLILTAKIVTKEELGFLKKNNIHQLIRKGDI"
     gene            complement(2509..3333)
                     /locus_tag="PKZ74_11720"
     CDS             complement(2509..3333)
                     /locus_tag="PKZ74_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPM93465.1"
                     /translation="MNTRIPAELKDIMADVIYTYDESFLMNIMAMRIEFTQCNSVPEY
                     VDLMMNDEEEQKIFRSSLTVNYTGFFRNPVAFAYLKSIILPHLILDKINHNKKLIRIW
                     AAGCSTGQEAYSLAFLLSDLEPVISRPFSWMIFATDNQPENLRKAEAALYSDDEMENL
                     TLGHARKYFTKEKSDHRVIRKINERINFSHSDLLNSSNSTPVSAIFGNFDVIICANLM
                     MYYSATIRNRIQHTLFRALDDNGYLIIGESEHDRIINDSFQEVYPQSFIFRKIQKL"
     gene            complement(3346..4260)
                     /locus_tag="PKZ74_11725"
     CDS             complement(3346..4260)
                     /locus_tag="PKZ74_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPM93466.1"
                     /translation="METQVRILHIDDNLHDRQLVLDAFGELSEQFHIIEADSREKFEN
                     YLKHESFDLVLSDFNILGFDGFKVLEIVKEKNPDIPVIIVTGTGSEEIAVQAMKMGAD
                     DYVIKTASHIRNLVPTVRNVLEKRKIQREHKFVLEELRESEERFRLLFENSMDAVLLT
                     CPDGRTLKANPAACRMFGWSEKEMTEKGRSLIMDTSDPRLEPAVKLRNETGWFYGELT
                     GVRKDGVKFPIELSSAIFRDKEGNLNTSVIIRDISERKAAEEAMQKKISQLEHFHQLT
                     VGRELAMIELKKEVNELLRQLGKPEKYT"
     gene            complement(4290..4733)
                     /locus_tag="PKZ74_11730"
     CDS             complement(4290..4733)
                     /locus_tag="PKZ74_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM93467.1"
                     /translation="MNRLAQILLVEDNEMDVVLTLDAFREARLKNKIIVARNGEEALE
                     YLFGRGEFSDRKSFPLPNLILLDLKMPKIDGFEVLRQIKATPKLKRIPVIILTSSKED
                     GDRILSYDIGANSYLAKPVSFDGFIEVVRKIDDYWFTLNIEAPPG"
     gene            complement(4742..>5288)
                     /locus_tag="PKZ74_11735"
     CDS             complement(4742..>5288)
                     /locus_tag="PKZ74_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93468.1"
                     /translation="MAFIGEASQRMENLINDLLGYSRLVRKTPDLQRVSLKDLYESIL
                     MVFKQELDKAGARIAIQDNLPEIVSDKTLLQQILTNLVSNAIKYRRGNVPLQIYCGYN
                     KEGASHVLFVSDNGIGIPEEYKMKIFEVFQRLHSENEYPGTGIGLASVKKAVALLDGK
                     IRVESVVNEGSTFYIKLPENK"
BASE COUNT         1252 a         1247 c         1208 g         1581 t
ORIGIN      
        1 caatatctcc tttccggatc agttgatgga tattgttttt cttcaggaat cccagttctt
       61 cctttgttac tatcttagcc gtcaggatca aaaccggcac gtatgccgtt ttttccgatt
      121 cccgaacagc ctggagcaca tcaaagccac ttttaccggg catcatcagg tcgagtatta
      181 tcgcatcagg cgtaaaagag ttcatccttt ccagggcctc atctccatct cgtgccacaa
      241 tcacattgaa tccctgctgg cttagcacat cctgaatctg gatgatggcc ggctcgctgt
      301 cttcaatcaa cattatggat ccggccagtt caggtgagtc aggtgtgcct gccgtttttt
      361 tcaaatgata tggattacct gccgaataat cgaacggtcc tgattcctct ccggttgata
      421 aatgtacggg tattgacagt gtaaaatcgg atccttttcc cacctggctc tgaacattta
      481 tttttccatt gatcaggtcg gcatattttt tggccagcgc caatcccaat cccgtaccgc
      541 cgtatcgttt cgatgtgctg ctgtcgccct gcctgaactc atcgaaaata tagggcaggt
      601 gctcttcggc gatgccaatc ccggtatctt ttatagttat accgacttta tcatccaggt
      661 gtcttgtgct tatttgcaca cctcctttgt cggtgaactt tacggcattg ccgataatat
      721 tctgcaggat gtggtagatc ttcccggcat cgctgtgaat gaccatcgat tctttttcgg
      781 gggcaaaatc gatggtgata tttttctgag aggcttgcgg agcgatcatg tttacgatgc
      841 cggagatcag ttccgtcagg ttaaaatcgg taaattccat ttccaccctt cccgattcga
      901 tccgggaaat atccaggata tcgttgatca gggccaggag gtttttcccg tttcgttcaa
      961 tgacttccag gtagctatgc tcttcggcag ggatcttacc ttcgagcctg cggtttaaaa
     1021 cccccgtaag ggcgatgacc gaattgaggg gagttcgaag ctcgtggctc atattcgaca
     1081 ggaaattcgt tttgagccgg ctggcttcgg ccaggtgctg tttttgtgtc tccagttcgg
     1141 tattctgcct gccgaggatg gtggattgcg tcatgagttc attcttttgc atctccagtt
     1201 cctggttctg gatttcgagt ttagccgaca gctcccggac tttctggtaa gacaatatac
     1261 cttcgatcct ggcgcttaag gcggccagta tttccttcac cagctccgaa tcctgtttac
     1321 tgaagttttt tatcgtcgcc aatgagatga cggcaatgac atcatcgccg acagcaaccg
     1381 gcgcggtaat aatttcgcgg ggtgtgatca taccgccggc tgccggaaaa cgcatcggcg
     1441 tgtcgccagg tatgtgggtg atatgctgaa ctttacgcgt ggttacagcc aggccgatat
     1501 cgccttccag cacctctgcc gggaatgatt tcctggccgg ttcttccaaa ccgatcgact
     1561 caaacaggtc gaacgtctgc gatggtttgt tatacaggta gaccgtggcg atttgtgctc
     1621 cggtagattc acacaaccgt gtcatgatcg ttctgaaaaa caaccgggga tcgttctccg
     1681 ccagcatcag ccgttcgatc tgttcggttt tttgattcag aatgatgatc gattcgagat
     1741 aatctgccat gtcgttaaaa gcaccggaaa gtaagccgaa ttcattactg ctatgatatt
     1801 ggcttcggat ccggtaattt ccttccctga attgttgtgt tgcggaactc agttcatcca
     1861 atggttggcg gatatttctg atcaggatat taaaaatgat gatggaaacg agcatagaga
     1921 tgatgatgac gatcaccagc ctgatattca actttctgct caggatttgt gagtcttcat
     1981 acaattgatc cgctttgttt ttcgcaaaag tatcgatgtc gcctataaaa tgaaacacaa
     2041 caccaacatc acgattaagc gcattcaaga cctggagact gttttgtgtc cggccggaat
     2101 cggtgttcag gatgataccg attttttcat cgcgggtggc attccacctt gagaaagcct
     2161 ctttcaacga atcgacatcg gattttggcc ccaggtaatg caattccagg atctcgattt
     2221 gcctggcagc gtcttcgcgg gcatgcatga tttgaatgag gttttcgctc acaacatcat
     2281 ggtcatcgaa gagcatcagg ttcctgacat ccctttgaat ggaaacaatg tcggtagtca
     2341 aatggccgat ggcgcgcctg accttgaaag gatgattata gatcagttct gtttgttcat
     2401 ggatcagcct gctctggtta taggctataa ttccggtaat gaaggccatg agtaacatga
     2461 ggccgaggcc ggcgatgagt agtattctga tttttatatt cctcaggttc ataatttttg
     2521 gattttccgg aagatgaaag attgaggata aacctcctga aatgaatcat taattatcct
     2581 gtcgtgttca gattcaccga taatgaggta gccattatcg tccaatgccc tgaacagcgt
     2641 gtgctggatc cggttccgga tcgttgcgct atagtacatc atcaggttgg cacagatgat
     2701 aacatcaaaa ttcccgaata ttgcggatac cggagtggag ttggagctat tcaacaggtc
     2761 ggaatgggag aaattgatcc gttcgttgat ttttcgaatg acgcggtgat cagatttctc
     2821 tttcgtgaag tatttccggg catgtcccag ggtcagattt tccatctcat cgtcggaata
     2881 cagggctgct tcagcctttc gaagattttc cggctggttg tcggtggcaa agatcatcca
     2941 ggagaatggc cgggaaataa caggttccag atcggaaagg agaaaagcca ggctataggc
     3001 ttcctggccg gtagagcaac ccgccgccca gatcctgatc aactttttat tatgattgat
     3061 tttatccagg atcaaatgag gcaggatgat cgatttgaga taggcaaaag ccaccggatt
     3121 acggaagaag cccgtataat tcaccgtgag cgacgacctg aagatctttt gctcttcttc
     3181 gtcattcatc atgagatcaa cgtattccgg cacgctgttg cattgggtga attctattcg
     3241 catagccatg atgttcatga gaaatgactc gtcataagta taaatgacat cggccataat
     3301 atccttcagt tccgctggaa ttcttgtgtt catccggtat taatactagg tatatttttc
     3361 cggtttcccc agttgcctca acaattcgtt gacctctttt ttcagctcga tcatggccag
     3421 ctctcttccg acggtaagtt gatggaagtg ctcgagctgg ctgatctttt tctgcatggc
     3481 ctcctcggcc gctttccgtt cggagatatc gcgaatgata acgctcgtat taagattgcc
     3541 ttctttatcc cggaaaatag cgcttgacag ttcaattgga aacttcaccc cgtcttttct
     3601 gacgcctgtg agttccccgt aaaaccagcc cgtttcgttt ctgagtttaa cagccggttc
     3661 cagccgcgga tccgatgtgt ccatgatcaa acttctgcct ttttccgtca tttccttttc
     3721 agaccaaccg aacatacgac aagctgccgg gttggctttc agcgtcctgc catcggggca
     3781 tgtcagcaat acggcatcca tgctattttc aaacaataac ctgaacctct cttcgctctc
     3841 tcgtaattct tccaggacga acttatgctc ccgctgaatt tttctttttt ccagaacgtt
     3901 cctgacggtg ggcaccaggt ttcggatatg gctggccgtt ttgatcacat aatcgtcggc
     3961 gcccattttc atggcctgaa ccgcaatttc ttctgaaccg gttccagtca caataatgac
     4021 aggaatatcc ggattttttt cttttacaat ttccagaact ttgaatccat cgaatcccag
     4081 gatattaaaa tcgctgagaa ccaggtcaaa ggattcgtgt ttcagatagt tttcaaattt
     4141 ttccctgcta tcggcctcaa ttatatgaaa ctgttccgat aattcgccaa aggcgtcgag
     4201 aaccagttgc ctgtcgtgca ggttgtcgtc gatgtgtagg attctgactt gtgtttccat
     4261 ttttaaggga ttaatcagtg aataatctgt cagcccgggg gagcttcaat attaagggta
     4321 aaccaatagt cgtcgatttt cctgacaact tcgataaaac catcgaagga gaccggtttg
     4381 gccaggtaac tgttggcacc gatatcgtag ctaaggatac ggtcgccatc ttcttttgaa
     4441 gaagtcagga tgatcaccgg gatccgtttg agctttggtg tagccttgat ctgacgtaaa
     4501 acttcaaagc cgtctatttt tggcattttt agatccaaca gaatcaggtt cggaagcgga
     4561 aaagacttcc tgtcggagaa ttcacctctt ccaaataaat attccaatgc ctcttctcca
     4621 tttcgggcta caatgatttt gtttttcagc cgggcttccc ggaaggcatc cagggtaagg
     4681 accacatcca tttcgttgtc ttcgaccaat aaaatctgtg ctaatctgtt cataaccgat
     4741 tttatttatt ttcaggcaat ttgatataaa aggtacttcc ttcatttaca actgattcaa
     4801 cccggatttt accgtcgagc agggctactg ctttttttac ggaagccagg cctatacccg
     4861 tacccggata ttcattttca ctatgcaagc gttgaaacac ttcaaaaatt ttcatcttat
     4921 attcttcagg gataccaatg ccgttgtccg atacaaaaag tacatggctg gccccctctt
     4981 tattgtaacc gcaataaatc tgcaaaggga catttcccct gcggtatttg atggcatttg
     5041 acactaaatt agttaaaatc tgctgaagca aagttttatc actgacaatt tcgggcaggt
     5101 tatcctgaat ggctatgcgg gcgccggctt tatccaactc ctgtttgaag accattagga
     5161 tgctctcata gaggtctttc aacgagaccc tttgcagatc gggtgtttta cgcaccaaac
     5221 gggaatagcc gagcaggtcg ttgatcaggt tctccatgcg ctggctggct tcaccgataa
     5281 aagccatg
//
LOCUS       DAOPNH010000247         5271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18207_length_5271_cov_4.961465, whole genome shotgun sequence.
ACCESSION   DAOPNH010000247 DAOPNH010000000
VERSION     DAOPNH010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18207_length_5271_cov_4.961465"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..863
                     /locus_tag="PKZ74_11740"
     CDS             57..863
                     /locus_tag="PKZ74_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93469.1"
                     /translation="MNSIRYTEWIDSYVEGELDEAGMRQFEEELEINRHLALEYKLDK
                     DINEALLEEDILDLRAKCMTAQNEYNLSRSRIARVVQFTRKYWYAAASVILIGLIAGG
                     LMLMNPGGYSSEKLFKMYYKSDPSVGISRSGNVNMVEALRVFSMNDYKAADKLFDKVL
                     ETDPGNIPVKYYSGISNIENRNFEKAIAMFDDIIIDGDNLYVENAQWYLGLSQLAAGN
                     IKDAVEIFGIISRKQDHFYHKDAESILEKINKSGNNKNILNKLFFLILPF"
     gene            1045..2223
                     /locus_tag="PKZ74_11745"
     CDS             1045..2223
                     /locus_tag="PKZ74_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM93470.1"
                     /translation="MNQRNSFITATPVFLAFLCMGFGDVVGPLTGLIKVEFQLSNFMA
                     QLIPFMGFIMFGLLSIPVGILQDKKGKATILLTGLVLALMGLLIPMTGKFSSFIAILG
                     GILLLGAGATFLQVSGNPLMRDVSAPGKYSRNLSLAQFIKAIGSLSGALIPLAAANYW
                     GGDWKLLFPVYSIVIAIVLLWLSLSGIRKTEVRENREKPATFRSSFSLLRNRYVATMV
                     TGIFLYVGAEVSMSSGLPIFLQDAFQLDIARMGLLGTLFFFLALTAGRFMGALVLNWI
                     KARTFLIISVVFSMFGFLGVFSGVQTLAMVGIFVIGLGFANIFPLIFSTTVETMPERT
                     NELSGLMVAAIIGGAIIPMLMGLVADNSSTSFAFIVPLACVIFILVASLIKPVRLIYN
                     "
     gene            2225..3202
                     /locus_tag="PKZ74_11750"
     CDS             2225..3202
                     /locus_tag="PKZ74_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HPM93471.1"
                     /translation="MNRRKFLKNGTAATAGILLYPGKNAGSALPLEPNDSGKRVLLST
                     WDFGQKANEKGWNCLLKGKKALDAVEEGIRVIEEDPDNLSVGLGGLPDRDGYVTLDAC
                     IMNEKGRAGSVTFLRDIVHPISVARLVMEKTPHVMLSGAGALQFALENGYKRENLLTE
                     KARKAWEEWKTTSHYQPVINIENHDTVGLLVLDSEGNLSGGCSTSGLAYKMHGRVGDS
                     PIIGAGLYVDNKVGAAVATGLGELVMRSLTSFLVVELMRNGSSPQKACESAIERIIMN
                     NPEYGEVQVGIIALDKSGNHGAFAIQPGFTYALRDENRDEVLASNSYLQ"
     gene            3199..>5271
                     /locus_tag="PKZ74_11755"
     CDS             3199..>5271
                     /locus_tag="PKZ74_11755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM93472.1"
                     /translation="MRGMIKSAALLLVLVFTVGCTQTGDFNPVEFVNPFIGTDGHGHT
                     FPGAALPFGMVQLSPDTRKDNWDACSGYHYSDSVILGFSHTHLSGTGVGDYGDIRFMP
                     LTGKLYNRPGNHELAETGYRSGFSHDSEEASPGYYKVYLTDHKTGVELTATRRCGFHK
                     YTFPSSDESHILIDLKESVVSEKILESSVRIINDHEISGFRRTSGWAEDQVVYFYAEF
                     SKPFSASGIFTNDTLSESNQADGKDIQAFVRYKTHEAEALLVKVGISAVTEEGALKNL
                     RAEIPDWDFERVRNSAADEWNRELGKIEVRDKNRDNLTKFYTALYHAFLAPNLFSDTD
                     GRYRGHDGAIHHGGFDVYTVFSLWDTYRALHPLMTIVQPERTNDFIKTFIDIYEKGGL
                     LPVWELAGNETFCMIGYHAVPVIADAYLKGYRGFDHEKAFAAMKGSAGQDHYGLDSYR
                     KFGFIPAEAEGEAVSKTLEYAYDDWCIAKVAAQMGREEDYRNFIMRAQYYKNLFDPET
                     GFLRGKRNGMFAEPFDPAEVNFMLTEANTWQYTFYAPQDISGLMALMGGKEQFENRLD
                     EMFSAPENLSGRQQADITGLIGQYAHGNEPSHHMAYLYNYTGSPDKTQRLVRQIMDEL
                     YGTGPDGLCGNEDCGQMSAWFVLSAMGFYPVTPGSGYYVIGSPLFDEVILHLEGGKTF
                     QISARGNDE"
BASE COUNT         1427 a         1056 c         1312 g         1476 t
ORIGIN      
        1 gaaattaaag gaaatgatta taaacgatcc gtattttaaa aagtatttat ctgattatga
       61 atagcataag gtatactgaa tggattgaca gctatgttga aggtgaactg gatgaagccg
      121 gaatgcgaca atttgaagaa gaattggaaa ttaaccggca cctggccctt gaatataagc
      181 tggataagga cattaacgag gcactcctcg aagaagatat ccttgacctg agggcgaaat
      241 gtatgactgc ccagaatgaa tacaaccttt cccggagccg aattgcccgg gtggtgcagt
      301 tcacgcggaa atactggtat gcagctgcat cagttattct gataggtctt attgccggcg
      361 gcctgatgct gatgaatccc ggcgggtatt cgtcggagaa attatttaaa atgtattaca
      421 aatctgaccc atcggttgga atatccaggt ccggtaatgt aaatatggtt gaagctctca
      481 gggtcttcag catgaatgat tataaagccg ccgataaatt gtttgacaag gttctcgaaa
      541 ctgaccccgg aaacatacct gttaaatact attccgggat ttcgaatatt gaaaacagga
      601 attttgagaa ggccattgcc atgttcgacg atataatcat cgatggagat aatctatatg
      661 tcgaaaatgc acagtggtac ctgggcttgt cacaattggc agctggaaat attaaagatg
      721 ccgtagagat atttggaatt atttccagga aacaggacca tttttaccat aaagatgctg
      781 agagtatcct tgaaaagata aataaaagcg gaaataataa aaatatttta aataaattgt
      841 tctttttgat attacctttt taatttttca ggattaactt cagattgaag aaataatcct
      901 tttgtttttt catgattttg ggtttaagta aagggtgccg gggggcaccc tttatttttt
      961 tcaaactttt ccagcctgct ttccttattg ttaaaactaa tatcttcgcc ttatctaatc
     1021 ataaagcagt aaatcttaat tattatgaac caaaggaata gttttataac tgcaactccg
     1081 gtttttcttg ctttcttatg tatgggtttc ggcgatgttg ttggcccact caccggcctt
     1141 ataaaagttg aattccagct ttcaaatttc atggcccagt tgataccttt tatgggtttt
     1201 atcatgttcg gattgctttc cattccggtg ggcatattgc aggataaaaa aggtaaagca
     1261 acaatattgc tgacaggact ggtcctggcc ctgatgggac tgcttatccc gatgacgggc
     1321 aaattttctt cattcatagc tattcttggc ggtatcttgc tattgggtgc cggcgctact
     1381 tttctccagg tttcgggaaa tccgctgatg agagatgtat cggcaccggg aaaatattcc
     1441 cgaaaccttt ctcttgccca gttcattaaa gcaataggct cactttccgg cgcattaatt
     1501 ccgctggctg ctgccaatta ctggggtgga gactggaaac tgttatttcc tgtatattcc
     1561 attgttattg ctattgtgtt gctatggttg tcgctttcag ggatcagaaa aaccgaagtg
     1621 cgtgagaaca gggaaaaacc agccactttc agatccagct tttccttact tcgtaaccgt
     1681 tatgttgcca ccatggtgac agggattttc ctgtatgtcg gagcggaggt gtcgatgagc
     1741 tcaggcctgc cgatatttct gcaggatgct tttcaacttg atattgccag gatgggtttg
     1801 ctgggcacat tgttcttttt cctggccctg acagccggcc ggttcatggg agcactggta
     1861 ctgaactgga taaaggcgag aacttttctt atcatttccg ttgtattttc tatgtttggt
     1921 ttcctgggcg tgttttcagg ggttcaaacc ctggcaatgg tcggtatatt tgtgatagga
     1981 ctgggttttg ccaatatttt ccccttaatt ttcagtacca cagtggaaac tatgcctgag
     2041 agaaccaatg agttgtcggg actgatggtg gccgcaatca taggcggggc tattattccc
     2101 atgcttatgg gtttggtagc agataacagc agtacatcct ttgcgtttat cgtaccatta
     2161 gcctgtgtca tttttatttt agtcgcttcg cttatcaaac ccgtcaggct catatataac
     2221 tgatatgaac aggaggaaat ttttaaagaa tggtactgcc gctacggctg gcattctact
     2281 ttatccggga aagaatgcag ggagtgcatt acctttagaa cctaatgatt cgggtaaaag
     2341 agttctactc tcaacatggg attttgggca gaaagccaat gaaaaaggat ggaattgcct
     2401 tcttaaggga aaaaaggcac tggatgcagt ggaagaagga attcgcgtca tcgaagaaga
     2461 tccggataat ttgtcggtgg gtttgggcgg tttgcccgac cgcgatggtt atgtcaccct
     2521 cgatgcatgc attatgaatg aaaaggggag ggcgggctca gtgaccttct tgcgggatat
     2581 tgttcacccg atctcagtgg cccggcttgt catggagaaa acgcctcatg tcatgctttc
     2641 aggggctggt gcattgcaat tcgcccttga aaatggctat aaaagagaaa accttctgac
     2701 tgaaaaggcc aggaaagcgt gggaagaatg gaaaacgacc agccactacc aaccggttat
     2761 caatattgaa aaccacgata cggttggctt attggttctg gacagtgaag gaaacctgtc
     2821 ggggggatgc tccacgagcg gattagccta caaaatgcat ggccgggttg gtgattcacc
     2881 catcattggc gccgggcttt atgtcgacaa caaagttggg gctgcagttg caacaggtct
     2941 gggagaactg gtgatgaggt ccctgacctc ctttcttgta gtggagctga tgcgcaatgg
     3001 atcgtcccct cagaaagcgt gtgaatcggc tatcgaaagg atcatcatga ataatccgga
     3061 atatggcgag gtccaggttg gtatcatagc tcttgacaaa tcgggtaacc acggcgcttt
     3121 tgcgattcag cccggcttta cgtatgcctt aagagatgaa aaccgggatg aagttttagc
     3181 atcaaatagt tatttacaat gagaggaatg attaaaagcg cggccctcct gctggttctt
     3241 gtattcacgg taggatgtac ccaaacaggc gatttcaatc cggtcgaatt cgtcaatcca
     3301 tttattggta cagacggcca tggccataca tttcccggag ctgccctgcc tttcggaatg
     3361 gtgcaactta gtccggatac gcgcaaagac aactgggacg cttgttcggg ataccattat
     3421 tcagactccg tcattctggg tttttctcat actcacctaa gcggaacggg agtaggcgat
     3481 tatggtgata tcaggttcat gccgttgacc ggcaaattgt ataaccggcc cgggaaccac
     3541 gaactggcgg agacgggtta ccggtcaggc ttttctcatg attcggagga agcatctccg
     3601 ggatattata aggtatattt aacagaccac aaaaccggag tcgagctgac ggccaccagg
     3661 cgttgcggat tccataagta tacttttcca tcttcggatg aatcgcatat actcatcgat
     3721 ttaaaggagt cggttgtctc tgagaagatt ctggaatcat ccgttagaat cattaatgat
     3781 catgagatca gcggatttcg gagaacttcg ggttgggcgg aggatcaggt ggtctatttt
     3841 tatgctgagt tttccaaacc cttttcagcc tccggaatat tcaccaatga cacattatcc
     3901 gaaagcaacc aggcagacgg taaagatata caggcttttg tcaggtataa aactcatgag
     3961 gccgaagctc tcctggttaa agtcgggatt tcggctgtga ctgaagaagg cgcgctcaaa
     4021 aatctgagag ctgagatacc ggactgggat tttgaaaggg tgagaaattc agcagccgac
     4081 gaatggaatc gtgaattggg aaagatagag gtgcgtgata aaaacaggga taacctgact
     4141 aagttttata cagctttata tcacgctttt cttgctccga acctgttttc tgatacggat
     4201 ggccgttacc gtggtcacga cggagccatt caccatggcg ggtttgatgt ttacactgtt
     4261 ttttccctct gggatactta ccgggcatta cacccattaa tgaccatcgt tcaaccggaa
     4321 agaaccaatg attttatcaa aactttcatc gatatttatg aaaagggggg cttgcttccg
     4381 gtttgggaac tggctggcaa tgaaacattc tgcatgatcg gctaccatgc agtccctgtt
     4441 attgccgatg cttatctgaa aggttataga ggatttgatc acgagaaagc atttgctgcg
     4501 atgaagggga gcgccggaca ggaccattac ggattggata gttaccggaa atttggtttt
     4561 atcccggccg aagcagaagg agaagcagta tcaaaaacac tggaatatgc ctatgatgac
     4621 tggtgcatcg caaaagtggc ggcgcagatg ggcagggaag aagattacag gaacttcata
     4681 atgagagctc aatattataa aaatctgttt gatcccgaaa ccgggttttt gagagggaaa
     4741 cgaaatggaa tgtttgccga acctttcgat cctgctgaag tcaactttat gctgactgaa
     4801 gccaacacat ggcaatacac attttatgcg ccccaggata tttccggttt aatggcgctg
     4861 atgggaggca aagaacaatt tgaaaatagg ctggatgaaa tgttttcagc accggagaac
     4921 cttagtggac ggcaacaagc cgatataacc ggactaatcg gacagtatgc ccatggaaat
     4981 gaacccagtc accatatggc ctacttatat aattatacgg ggagcccgga taaaacccag
     5041 cggctggtca ggcaaatcat ggatgaactt tatggcaccg gtcccgatgg attatgcggg
     5101 aatgaagact gcggccagat gtctgcatgg tttgtcctca gtgccatggg attttacccg
     5161 gtaaccccgg gatccggata ttatgttatc ggaagcccct tgtttgatga ggtcattttg
     5221 catctcgaag gaggtaaaac ttttcaaata tccgccaggg gcaatgatga a
//
LOCUS       DAOPNH010000248         5266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18241_length_5266_cov_6.009979, whole genome shotgun sequence.
ACCESSION   DAOPNH010000248 DAOPNH010000000
VERSION     DAOPNH010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18241_length_5266_cov_6.009979"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..693)
                     /gene="rplI"
                     /locus_tag="PKZ74_11760"
     CDS             complement(244..693)
                     /gene="rplI"
                     /locus_tag="PKZ74_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPM93473.1"
                     /translation="MEVILIQDVANVGYKDDIVTVKNGYGRNYLIPQGMAILATETNK
                     KVIAENKKQKSYKEDKIRSEATTIAKTLETITLKIGAKAGTSGKIFGSVNAIQIANAL
                     KEQFNFEFDRKQIEVDGESIKELGTYKAKVKLHKEIAAEINFEVFAE"
     gene            complement(709..978)
                     /gene="rpsR"
                     /locus_tag="PKZ74_11765"
     CDS             complement(709..978)
                     /gene="rpsR"
                     /locus_tag="PKZ74_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPM93474.1"
                     /translation="MAQQTGDIKYLTPINVDTKKKKYCRFKKAGIKYIDYKDANFLLK
                     FVNEQGKILPRRLTGTSTKYQKKVAQAIKRARHLALMPYVADLLK"
     gene            complement(1006..1362)
                     /gene="rpsF"
                     /locus_tag="PKZ74_11770"
     CDS             complement(1006..1362)
                     /gene="rpsF"
                     /locus_tag="PKZ74_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPM93475.1"
                     /translation="MANQYETVFIMTPVLSDDQMKETVGKYQKLLKDKGAEIVLEDNW
                     GLRKLAYPIQKKSTGFYYLIEFKSEGDIIKDVELAFKRDERIIRFLTVKLDKHAVAYN
                     EKKRRKAQEEQQPTEA"
     gene            complement(1426..2592)
                     /gene="wecB"
                     /locus_tag="PKZ74_11775"
     CDS             complement(1426..2592)
                     /gene="wecB"
                     /locus_tag="PKZ74_11775"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPM93476.1"
                     /translation="MKRFRIISIVGARPQFIKVAAICRAAREHFAERIEHLIIHTGQH
                     YDENMSKVFFDEMEIPEPDFNLETGSGSHGFQTAGIMLRTEKVLEQEKPDIVIVYGDT
                     NSTLAGALAAAKMHIPVAHIEAGLRSFNKLMPEEINRVVADHVATLLFTPTKAGQENL
                     AREGFAEAVPPYSADNPAVFHCGDVMYDNALYYSSAAEKKTEMLDRLDLHDIPFILLT
                     IHRENNTDSADRLERILGTLNELSREYGITCVFPMHPRTRKMTAGRQFQKLMSEIGAN
                     LLFKVTGPLSYFETLLLEKNCQMVITDSGGVQKESFFFRKPCLVLRDGSEWSELVELG
                     TARLVDDDTAKIRASFAHFLKNPPTHFPGIYGDGRSAEFILGELVKFLTGGLHE"
     gene            complement(2632..4752)
                     /locus_tag="PKZ74_11780"
     CDS             complement(2632..4752)
                     /locus_tag="PKZ74_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPM93477.1"
                     /translation="MKKLLLLPAAILIPILCMAQGRFITPGEAGAVAVKWYIGHMPGQ
                     RFTDPSDVTIAESFEIGNENDIFYYVCNIAAGGFVAVSAHTANPPVLCYAFSGQYETK
                     NQPENFRAWMAQYQRQISFSLQQKALPTGETARDWDYYLNTPVETIVPFEGREVAPML
                     HSNWDQGMYYNQQCPADESGPGGHCVTGCVATAIGQLMNYFRWPDTGTGSYSYECPPY
                     GTLSADFGNSTYEWDKMEPLLIRGTAEAAELLFHTGVSVDMVYGPDGSGMYNHKGAYT
                     MKTYFKYSPETQYLFRDSTSMDWDSVIVAHLDRKIPMYYAGWSVPNVMGHAFICDGYQ
                     GEDYFHFNWGWGGQLDGYFYTDNLTPGGNQFNLAQELIINAVPDTNLYTYPTHCEGDK
                     VLTNLGGTFGDGSGPLYGYGNGASCSWLIAPADSVINITLEFLHFELEPGDTVTVYDG
                     DTVTAPVLGTFSGNELPTAITTESPEMLVVFNSDDGGSGEGFLAHYNTVTPVYCSLMV
                     DLTAQSDTLSDGSNNWNYHNNAACLFRIMPAGASTVTLYFTEFATEEGVDELKIFDMQ
                     TQELLATYSGTYGQGVPDPVTSYNGKLFVVFSSDYDISDAGWTAYYESNLVNIRESEP
                     NNTILVYPNPANDILHIKINPAMPENIPASLVDISGRQMAVDLQYNSAFDATMDLRNI
                     LPGIYFLRLELPGTAPFFEKIIVQ"
     gene            complement(4959..>5266)
                     /locus_tag="PKZ74_11785"
     CDS             complement(4959..>5266)
                     /locus_tag="PKZ74_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93478.1"
                     /translation="CTRNHLHLRYCISENSPVTELSVFPNPANDKLHVEFNVNSTEDL
                     KIKLVNITGQTLYSEIHPGFSGNYQNEIAVGSLPAGMYFLEINAVTGTANKKIFINR"
BASE COUNT         1246 a         1280 c         1291 g         1449 t
ORIGIN      
        1 gattccctgc ccgggaatgc ctgtagtgga atacgaagga caggtgtaca acacgatcca
       61 gattttcagc cagtgctggc tgaaagagaa cctgaacgtg ggagtgatgg tcccagtgtc
      121 cgatgcctca gggatttcta gccatttttg atactcccgg ttcatttcat cctgacaagc
      181 agagataaaa agaaagggca gccctgcaaa ggccgccctc ctcaaattta gaaaattatc
      241 ttcttactcg gcgaaaacct cgaagttgat ctccgctgca atctccttat gaagcttaac
      301 ctttgctttg taggtgccca attccttgat cgattcgccg tcgacttcga tctgtttgcg
      361 gtcgaattcg aaattgaact gttctttcaa tgcattggcg atctggatcg cgttcaccga
      421 accgaagatc ttgcccgaag tgccggcttt ggcgccgatc ttcagggtga tcgtttcgag
      481 ggttttggca atcgtggtcg cttcgctgcg gatcttatct tccttgtaag atttctgctt
      541 tttgttctcc gctatcacct ttttattggt ttcggtggcg agaatggcca ttccctgcgg
      601 gatgaggtag ttccttccgt agccgttctt cacggttacg atatcgtctt tatagccaac
      661 gttagctacg tcctggatca aaataacttc cataacttgt tcctcctctt atttaagtaa
      721 atcagctacg tagggcatta aagccaggtg acgtgccctc ttgattgcct gggccacctt
      781 tttctggtat ttggtcgatg tgccggtcag tcgccggggg agaatctttc cctgctcgtt
      841 cacaaacttc agcaggaaat tggcatcctt gtaatcaatg tacttgatgc ccgctttttt
      901 aaaacggcaa tacttcttct tcttggtatc gacgttgatc ggggtaagat atttaatatc
      961 tccggtttgc tgtgccatgg ttctaaacgt ttttaggttt atacattaag cttcggtggg
     1021 ttgctgttct tcctgcgctt ttctgcgctt cttttcattg tatgcaaccg catgtttatc
     1081 gagcttaacg gtcagaaagc ggataatgcg ctcgtcgcgt ttgaatgcca gttcaacgtc
     1141 cttgatgatg tcaccttcgg atttgaactc gatgaggtag taaaaccctg tggatttttt
     1201 ctggatggga taggccagct tgcgcagacc ccagttgtct tccagaacga tctcggctcc
     1261 tttgtctttc agtaatttct gatacttacc taccgtttcc ttcatctgat catcagataa
     1321 aacgggagtc ataatgaaaa cggtttcgta ctgatttgcc attgtttaat cgtcgttgtt
     1381 tatgaatttg ttttttaaaa tgggctgcaa aggtaggaaa aatttttatt cgtgcaagcc
     1441 cccggtgaga aatttaacga gttcaccgag aatgaattct gccgaacggc catcaccgta
     1501 aatgccggga aaatgggtgg gaggattctt caggaaatgg gcgaatgagg ccctgatctt
     1561 tgccgtgtca tcgtctacca gccgggctgt gcccagttca accagctcgc tccattccga
     1621 cccgtcacgc aataccaggc agggcttccg gaaaaaaaac gactctttct gcaccccgcc
     1681 cgaatcggtg atcaccattt ggcagttttt ctccagcaga agggtctcga aatagctgag
     1741 aggcccggtg accttaaata ggagattggc cccgatttct gacatcagct tctgaaattg
     1801 ccggcccgct gtcattttac gggtcctggg atgcatcggg aaaacgcagg taattccgta
     1861 ttcccggctc aattcattga gggtccccag gatccgctca agcctgtccg ccgaatcggt
     1921 gttgttctcc cggtggatgg tcagcaggat gaaagggatg tcatgcaaat ccaatctgtc
     1981 cagcatttct gttttcttct ccgcggcaga ggaataatac agtgcgttgt cgtacatcac
     2041 atcaccgcaa tgaaaaacag ccggattgtc ggccgaatag ggcgggacag cctcagcaaa
     2101 accttcccgg gccaggtttt cttgtcccgc ttttgtaggg gtgaatagca atgttgcgac
     2161 atggtcggct accaccctgt tgatctcctc cggcatcaat ttgttgaacg agcgcagacc
     2221 cgcttcgata tgggcgacgg gtatatgcat ttttgcggca gccagcgccc cggccagtgt
     2281 cgaatttgta tctccgtaaa cgatgacgat atccggtttc tcctgttcca gtactttttc
     2341 ggttcgcagc atgatcccgg cggtctggaa gccatgtgag cccgatccgg tttccagatt
     2401 gaaatcgggt tccggtatct ccatctcgtc gaaaaacacc ttcgacatgt tctcgtcgta
     2461 atgctgaccg gtgtggatga tcaggtgttc gatcctttct gcaaaatgct cccttgcagc
     2521 ccggcaaatg gcggctactt tgatgaactg aggccgggcc ccgacaatcg aaatgatcct
     2581 gaatcttttc attctaattt ttataatgat gataaccgca cgatgattga tttattgtac
     2641 gattattttt tcaaagaaag gagccgttcc cggaagttcc agcctcagaa aatagattcc
     2701 cggaaggatg tttcgcaagt ccatcgtcgc atcgaatgcg ctgttatatt gcaggtcaac
     2761 agccatttgc ctgccggata tatccaccag ggaagccggg atgttttccg gcatagccgg
     2821 atttattttg atatgaagaa tatcattggc gggattggga taaacaagaa ttgtgttgtt
     2881 tggttcggat tcccggatgt ttacgaggtt ggattcgtaa tacgccgtcc atcccgcatc
     2941 cgaaatgtca tagtcggaac tgaacaccac gaagagcttt ccgttgtaag atgtcacggg
     3001 atcgggcaca ccctgcccgt aggttccgga gtacgtggcc agaagctcct gtgtctgcat
     3061 atcgaaaatt ttcagttcat cgacaccctc ctcagttgca aactcagtaa aatagagcgt
     3121 tacggtagaa gcgcctgccg gcatgatcct gaaaaggcag gcggcattgt tgtggtaatt
     3181 ccagttgtta ctgccgtccg acagggtatc ggactgggct gtcaggtcaa ccatcagcga
     3241 acaatagacc ggggtgactg tgttgtaatg ggccagaaat ccttccccgc tgccaccatc
     3301 atcactgttg aaaacgacca gcatttccgg gctttccgtt gtaatggccg tggggagttc
     3361 gtttcctgag aaagtcccca gcaccggagc tgttacggta tcgccgtcat agaccgtaac
     3421 cgtatcgccg ggttccagct caaaatggag aaattccagg gtgatattta ttactgaatc
     3481 ggccggtgca atcagccatg aacaggaagc tccgtttccg taaccgtata gcggcccgct
     3541 cccgtcgcca aacgtgccgc ccaggttagt cagcacctta tccccttcac aatgagtcgg
     3601 atatgtataa agattggtat cgggaacggc attgatgatc agttcctggg ccaggttgaa
     3661 ctggtttcca cccggagtca aattgtcggt gtagaaatat ccgtcgagct gccctcccca
     3721 tccccagttg aaatgaaaat aatcttcccc ctggtaaccg tcgcagataa aggcatgccc
     3781 catcacattg ggaacagacc aaccggcgta gtacatcgga atcttccggt caagatgggc
     3841 gacgatcacg ctgtcccagt ccatactggt cgaatccctg aataaatatt gtgtttcagg
     3901 tgaatatttg aaataggtct tcatcgtata agcgcctttg tgattgtaca ttcccgaacc
     3961 atcgggcccg taaaccatat ccaccgaaac gccggtgtgg aagagaagtt cggctgcttc
     4021 agccgttccc cggataagga gaggctccat tttatcccat tcgtaagtcg aatttccgaa
     4081 gtcggcactg agcgttccat atggaggaca ctcgtaggaa taagatcccg tcccggtgtc
     4141 gggccagcgg aaatagttca tcagttgccc gatggcggtg gccacgcatc cggtaacaca
     4201 atgtcctccg ggacccgatt catccgccgg gcattgctgg ttgtaataca tcccctggtc
     4261 ccagttcgaa tgcagcatgg gcgccacttc gcggccctca aacggaacaa ttgtttctac
     4321 aggcgtattc agataataat cccaatcccg tgcggtttcg cctgtaggta aggctttttg
     4381 ttgtaaagaa aaggagatct ggcgctgata ttgcgccatc caggcccgga aattttcagg
     4441 ctggttcttt gtttcatatt ggcctgaaaa tgcatagcaa agtacgggag gattggctgt
     4501 gtgggcagaa actgccacaa aaccaccggc agcgatattg cagacataat aaaaaatgtc
     4561 gttttcattc ccgatctcaa aactttcggc aatggtcaca tccgaaggat cggtaaagcg
     4621 ctgaccgggc atgtgcccga tataccattt tacagcaaca gcgccggctt caccgggagt
     4681 gatgaacctg ccctgcgcca tacataagat gggaataaga atggcggcgg gtaagagtaa
     4741 tagctttttc atcctggtca ttttacgata taaatgtaca aagaagttta taccgagtct
     4801 gaactgtttt gcgaatttta acagatgttc tccccggcac aactcattct aagcagcttt
     4861 gctgaggcaa acctgcaaag aatgagtata aaaaaaagag gctgatctga tttttcagat
     4921 agcctctttt cagagaaaat gaattctaaa tctcagtatt acctgttaat gaagattttc
     4981 ttattggccg tgccggtaac ggcattgatt tcaaggaaat acatgcctgc aggcagtgag
     5041 ccgacggcga tctcattctg ataatttccg ctgaagcccg gatgtatttc tgaataaaga
     5101 gtctggcccg taatgttgac cagtttgatt ttcaggtctt ctgtgctgtt cacgttgaat
     5161 tccacatgaa gcttatcgtt ggccgggttc gggaaaacac tcagttcggt aaccggggaa
     5221 ttttccgata tacaatatcg taaatgcaga tgatttcgcg tgcagg
//
LOCUS       DAOPNH010000249         5254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18301_length_5254_cov_6.062897, whole genome shotgun sequence.
ACCESSION   DAOPNH010000249 DAOPNH010000000
VERSION     DAOPNH010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18301_length_5254_cov_6.062897"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="pnuC"
                     /locus_tag="PKZ74_11790"
     CDS             complement(<1..560)
                     /gene="pnuC"
                     /locus_tag="PKZ74_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPM93479.1"
                     /translation="MLISIFQTFYDNLLASSWLEAVAVAAGLMSVWYARREDILVYPT
                     GIVNVLIYVYLCYEVKLYADMSINIFYFGMSIYGWIKWTRKTSDNQTRPITVMNRKEW
                     ILSTGGLVLFFFALWYILQKYTDSNVPVWDALTTAIFIIGMWLMALKKVENWIFWIAG
                     DLISVPLYASKSLVLSSFQFAVFLALA"
     gene            complement(560..817)
                     /locus_tag="PKZ74_11795"
     CDS             complement(560..817)
                     /locus_tag="PKZ74_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93480.1"
                     /translation="MKTSVEISYYPLDQEYVPPIKDFIGRMNRYEGLIVRTNGMSTQI
                     FGDYDCVMNALKDEIKISFQNPHSVFVMKVINTDLNTKEKE"
     gene            921..1475
                     /locus_tag="PKZ74_11800"
     CDS             921..1475
                     /locus_tag="PKZ74_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93481.1"
                     /translation="MKKNYFIWIGLIMTVLFLSGCLTCEKKEYTFQFTGKDSGRLTIK
                     YINILSTMDDTIDVSEEDFASLMTDYIEGSEIENEFPDATVVDKRLFEENGVLCGEIV
                     LEFSNLTACHLYQHKGKGPIMYCLGCYSIDSEYYGSSNGEYGNDIMPVVFWEDGLKTL
                     TLTTDVTTPDETTVSLLDRWKLGY"
     gene            complement(1478..2122)
                     /locus_tag="PKZ74_11805"
     CDS             complement(1478..2122)
                     /locus_tag="PKZ74_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM93482.1"
                     /translation="MRILSFIMIAAFVIAGHATHAQAKKTSEEILKQVSEKTKSFSSI
                     KIGFTYNMDNASAKIHETETGILTVKGDKYRLDIAGQRVISDSKTSWTYVADANEVQI
                     NTVEDDDNALTPTKLLTSYSENYKSKLVNEINKDGRAVYVIDLKPNTEKNFTNVELQI
                     DKELYRIVRIAIQDKSGNTFSYIVNKFEPNVPVKDTDFTFDPKEFPGVEVIDMR"
     gene            complement(2138..4549)
                     /locus_tag="PKZ74_11810"
     CDS             complement(2138..4549)
                     /locus_tag="PKZ74_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPM93483.1"
                     /translation="MARNTNGDKAEQGTRPKKEKSRDPEKNKKRLPAAFTDGRFARIM
                     GFFFLLSGVFLLLAFTSYFMSWKLDSNVLDNNSAGYIFDSSVTVHNWTGKLGAWFAHI
                     FINRGIGIAAYLLLFLMILTGFRLTTGIALLPFGKAFRYSLLGILWISVTLGFALRHN
                     PDMQLMGGVFGYESSKWLTGLLGAPGTVLLLAFILISFIVIINLIPSLTKKKQPATAD
                     EALDGEQGVSGVLKEDVYNTREYAVSPEPEAVPTEFTLYHREKEAPPEKPAPTDKGIE
                     LTIEETPKEEPVSPKEPERFGLDTLYDPTKDLSDYKYPTLDLLSDYGSDSVPVAKEEL
                     EQNKDKIVDTLNNYAIRIDKIKATIGPAVTLYEIIPAPGVRIAKIKNLEDDIALSLSA
                     LGIRIIAPIPGKGTIGIEVPNLKPEIVSIKSLLSSDKFKNSTFELPIALGKTISNESY
                     IADLTKMPHILMAGATGQGKSVGLNAILLSLLYKKHPSQLKFVMVDPKKVELTLFHKI
                     ERHFLAKLPDSEESIITDTRKVVRTLNSLCIEMDHRYELLKDAQVRNIKEYNAKFTSR
                     HLNPEEGHMFMPYIVMVVDEFADLIMTAGREIENPLTRLAQLARATGIHLVIATQRPS
                     VNIITGTIKANFPARFAYRVISKVDSRTILDSSGADQLVGKGDMLISTGSDLLRVQCA
                     FVDTPEVEKVTEFIGSQRGYPEAFALPEYYDENDEQEKGDFDPSARDELFEDAARIIV
                     QHQQGSTSLLQRKLKLGYNRAGRIIDQLEAAKIVGPFEGSKAREVLVANEMALEQFLK
                     DLYTK"
     gene            complement(4639..>5254)
                     /locus_tag="PKZ74_11815"
     CDS             complement(4639..>5254)
                     /locus_tag="PKZ74_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPM93484.1"
                     /translation="TSFATFAKEFKGVITYKVTIEGADVTDEMKAYLPKSMTMTIKGN
                     KSRTELIMSMMKTVSIADGDNKTAITLMDMMGQKFAIQSNYEEIMKEMGEAPEAKVEV
                     TGETKEIAGYVCKKAIITIAENDQELIVYFTDELGSSELNFDNPMYKDINGVMLEFEM
                     PNESFSMKFEATSVEKKNVDDSEFTIPEGYQVKTQEELKGMFGG"
BASE COUNT         1276 a         1243 c         1253 g         1482 t
ORIGIN      
        1 gccagcgcca ggaatacggc aaattgaaag ctgctcaata ccagcgattt actggcatat
       61 aagggaacgg atatcaggtc tccggctatc cagaagatcc agttctccac cttcttcaat
      121 gccatcagcc acatgccgat gatgaatata gccgtagtca gggcatccca taccggtacg
      181 ttgctgtcgg tgtacttctg taaaatgtac cataatgcga agaaaaaaag gaccaaaccg
      241 cctgtcgaca agatccactc ttttcgattc attaccgtaa ttggcctggt ttggttatca
      301 gatgttttcc tcgtccattt gatccagccg taaatgctca tcccaaaata aaaaatattg
      361 atcgacatat cggcatacaa cttcacctca taacacaggt aaacatagat caatacattc
      421 acaattccgg tagggtaaac caggatatct tccctgcgcg cataccagac gctcatgaga
      481 cctgccgcga ccgccacggc ttccagccat gacgaagcca gcaggttgtc ataaaaagtc
      541 tggaaaatgg agatcagcat tactcttttt ctttcgtatt gaggtcggta ttaatgactt
      601 tcatcacaaa aacggaatgt ggattctgaa aagaaatttt tatttcatcc ttcaaagcat
      661 tcattacaca gtcgtaatcg ccgaatatct gcgtactcat tccatttgtt ctgacgataa
      721 gcccttcata ccggttcatc ctgccgataa aatcctttat gggcggcaca tattcctggt
      781 caaggggata ataactgatt tcaacggatg ttttcatctc ttatattttt cacgaaatta
      841 aaaataatga aacactttta ttattgaagg atatccttaa atttgtttga agaaattccg
      901 cgcaataaac caagaatcat atgaaaaaga actattttat ttggatcggc ctgataatga
      961 ccgttttatt tctcagcggc tgccttacct gcgagaaaaa agaatacact ttccagttta
     1021 ccggcaaaga ttctggcaga ctgacgataa aatatattaa tatcctgtcg acgatggacg
     1081 atacgatcga tgtatcggaa gaggatttcg ccagcctgat gaccgactac attgagggct
     1141 cggagattga aaatgagttc cctgatgcta cggtagtcga taaaaggtta tttgaagaaa
     1201 atggcgtgct ttgtggcgag atcgtgcttg agttttcaaa tttaacggca tgccacctgt
     1261 accagcacaa aggcaaaggc cctatcatgt attgtctcgg atgttattcc attgattccg
     1321 aatactatgg ctcctcaaat ggagagtatg gaaacgatat catgccggtg gtattctggg
     1381 aagatggcct gaaaacgctg acgctaacga ccgatgtgac cacaccggat gaaacgaccg
     1441 tcagcctgct cgatcgctgg aagctgggct actgatctta tctcatatcg atcacttcca
     1501 caccgggaaa ctctttcgga tcgaaagtga aatcggtatc ttttaccgga acgttcggct
     1561 cgaatttgtt gacaatataa ctgaacgtat tcccgctctt gtcctgtatg gcaatcctca
     1621 caatccggta tagctccttg tcgatctgca gttccacgtt ggtgaaattc ttctcggtat
     1681 tgggttttag atcgataaca taaacagccc tgccgtcttt gttaatctca ttgaccagtt
     1741 ttgatttgta attctcactg tatgaggtca gaagctttgt gggagtaagg gcattgtcat
     1801 catcttccac cgtgttgatc tgaacttcat tggcatcggc gacataggtc catgatgttt
     1861 tgctgtcgga tataactcgc tggcccgcaa tatcaagcct gtatttatca ccttttacag
     1921 tcagtatgcc ggtttcggtt tcgtgtatct tggccgaagc gttgtccata ttgtacgtaa
     1981 atccaatttt tatggatgaa aatgattttg ttttctcgga tacttgtttt aatatctctt
     2041 cggatgtctt tttcgcttgt gcatgagtag catgccctgc gataacaaag gcagcaatca
     2101 tgatgaatga taaaattcgc atggtcaggt ttgttaatta tttagtgtaa aggtctttca
     2161 aaaactgttc caaagccatt tcgttggcga caagaacttc cctggctttg gagccttcga
     2221 acgggccgac gattttagcc gcttcgagct ggtcgatgat gcggccagcc cggttgtacc
     2281 ccagtttcaa tttccgttgc agcagcgacg tggagccttg ctggtgctgg acgatgatcc
     2341 gggcggcatc ctcgaacagt tcatcccggg cgctgggatc gaaatcgcct ttctcctgtt
     2401 cgtcattctc atcgtaatac tccggtaagg caaaggcctc cgggtagccg cgctgtgagc
     2461 cgatgaattc ggtcactttc tctacctccg gcgtgtcgac gaatgcgcat tgcacgcgca
     2521 acaggtcgct gccggtggag atcagcatat cgccttttcc gacgagctgg tcggccccgg
     2581 aactgtcgag gatggtacgg gagtcgactt tcgaaatgac gcggtaggca aaacgggccg
     2641 ggaagttggc tttgatcgta ccggtaatga tgttgaccga tggccgctga gtggcgatga
     2701 caagatggat accggttgca cgggctaatt gtgccagccg ggtcaaagga ttttcgatct
     2761 ccctccccgc agtcatgatc aggtcggcaa attcatccac caccatcacg atgtaaggca
     2821 tgaacatatg gccctcttcg ggattcaggt gccgggatgt gaatttggcg ttgtattctt
     2881 tgatattgcg cacctgggcg tctttcagca gctcataccg gtgatccatc tcgatgcaca
     2941 acgaattcag ggtccggaca accttgcgcg tatcggtgat gatcgattct tccgaatcgg
     3001 gcagcttggc caggaaatgc cgttcgatct tgtggaaaag ggtcagctcg acttttttcg
     3061 ggtcgaccat gacgaacttg agctggctgg gatgtttctt atacagcaac gaaagcagga
     3121 tagcgttcag gccgaccgat ttgccctggc cagtagcgcc ggccatcaaa atgtgcggca
     3181 ttttggtcag atcggcgatg tagctttcgt tggagatcgt tttgccaagg gcaatgggga
     3241 gttcgaaggt ggagttcttg aatttgtcgg aagataacag cgatttgatc gatacgatct
     3301 ccggtttgag gttcggcacc tcgataccga tcgtcccttt accggggatc ggtgcgatga
     3361 ttcggattcc cagcgcggag agcgataagg caatgtcgtc ttccaggttc ttgatcttgg
     3421 caatccgcac tccgggtgca gggatgatct cgtaaagcgt caccgccgga ccgatcgtcg
     3481 ctttgatctt atcaatccgg atggcgtaat tattcaaagt atcaacgatc ttatccttgt
     3541 tctgctccag ctcctctttg gctaccggaa ctgagtcgga gccatagtcc gacaatagat
     3601 cgagggttgg gtacttgtaa tcggaaagat cttttgtcgg gtcatacagt gtatcgaggc
     3661 cgaatctttc cggttctttc gggctgaccg gctcttcttt tggcgtctcc tcaatcgtca
     3721 gttcaattcc tttatctgtc ggtgccggtt tttccggagg agcttctttt tcccggtgat
     3781 ataaggtgaa ctcggtcggt accgcttcgg gctcagggct caccgcatat tcccgtgtgt
     3841 tgtaaacatc ttccttaaga acaccactga ccccctgttc gccgtcgagg gcttcatcag
     3901 ccgtagcggg ttgtttcttt ttagtgagtg aaggtatcag gttgataatg acgataaaac
     3961 tgatcaggat aaaggccagg agcagcacgg tgccgggggc cccgagcagg cctgtcagcc
     4021 attttgagga ttcatagccg aaaacgcctc ccatgagttg catgtcggga ttgtgacgga
     4081 gggcaaaccc gagagttact gaaatccaga ggatgcccag caaactgtaa cggaacgctt
     4141 tgccaaaagg cagtaatgca atgcctgtcg tcagccggaa accggtgagg atcatcagga
     4201 aaagcaacaa atacgctgca ataccgatgc cccggttgat aaagatatgg gcaaaccagg
     4261 ctcccagctt gcctgtccag ttatggaccg ttactgaact gtcaaagatg taccccgcac
     4321 tgttgttgtc gagaacattg ctgtcaagtt tccacgacat gaaataagag gtgaaagcaa
     4381 gaagcaggaa aacgcccgag agcaaaaaga aaaatcccat gatcctggca aaacggccgt
     4441 cggtaaatgc cgccggcaaa cgttttttat tcttttcagg atcgcgggac ttttcttttt
     4501 tcggccgggt tccctgttca gctttgtccc cgttggtatt tctggccatt gtggtttttt
     4561 ttacagaacg gcaaaattaa gaaaaattgc cctgcctgtc gatttacttt ttgaacagga
     4621 gtaatggatg cttacttctc agccgccgaa cattcctttc agctcttcct gggttttaac
     4681 ctggtagcct tccggaatgg tgaattcact gtcatcaaca tttttcttct ccaccgaggt
     4741 ggcttcgaac ttcatgctga aactttcatt gggcatctcg aactccagca tgacgccgtt
     4801 gatgtccttg tacatcggat tatcgaagtt cagctcgctc gatcccagtt cgtccgtaaa
     4861 ataaacgatc agttcctggt cattctctgc gatcgtaata atcgcttttt tgcagacata
     4921 accggctatt tccttggttt cgccggtgac ctccactttt gcctcgggtg cttcacccat
     4981 ctctttcatg atctcttcat aattcgactg aatggcaaat ttctggccca tcatatccat
     5041 cagggtaatg gctgttttat tgtcgccatc ggcaatggat acagtcttca tcatactcat
     5101 aatgagctcg gtgcgtgatt tgttgccttt gatggtcatc gtcatcgatt tgggcaggta
     5161 ggctttcatc tcatcggtta catcagcccc ttcaatagtt actttatagg tgatcacgcc
     5221 tttaaactcc ttggcaaaag ttgcaaaaga agtc
//
LOCUS       DAOPNH010000250         5156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18728_length_5156_cov_6.839051, whole genome shotgun sequence.
ACCESSION   DAOPNH010000250 DAOPNH010000000
VERSION     DAOPNH010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18728_length_5156_cov_6.839051"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="PKZ74_11820"
     CDS             <1..1600
                     /locus_tag="PKZ74_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPM93485.1"
                     /translation="APPPRVTTPSMVPSFGSLGPYDPNEGTIEGVVTLGGGAGSVTQV
                     TVTAGEYSTNPDNTGFYSMVVEEGTYDVIGTLQGYYPDTVFSVMVITGETTEDVDLTL
                     QALPTGFISGNVSLSEGNADLTEVEVSAGLHTVNPDANGDYIMEIEIGTYEVTASLDG
                     YMPETETGVVVVEGQTTTGVDFILDPVPTTGFITGIVLLAGGDGEVTEVNVTAGNVTV
                     NPGEDGIYLLEIVAGTYEVTASLPGYLTGVVPNVVVLVEQTTPDIDFYLYEAPDIGYI
                     EGWVTLNGPGDVTQAVVTAGNQSDNPNAQGYYFLGLPAGTYTVTADHPYTLPETLEGI
                     EVIAGQNTENVNFGLDVLRCNLSVKAVDQYGSLLSDVEVDIIGPEDIYTGIIENDSVI
                     FEDVPYGLYAGTAWLVMSPDTARTNDEINDSDQDMEFVFILGGMNDESQTRVRSFSIS
                     PNPFSEEVRLSFEVENPVEVILKVYSSTGRLIKILAEGRFDRGRHEVQWNGDEDGGNR
                     LPAGIYTVVFQAGGEREAKVLILR"
     gene            1867..2280
                     /locus_tag="PKZ74_11825"
     CDS             1867..2280
                     /locus_tag="PKZ74_11825"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPM93486.1"
                     /translation="MLALPFIVAGLTLNILYPEIFSVCGPTLGLIIASVIILIPGIII
                     WIWSVVLILTKIPRRELITTGPYALVKHPLYTGVALLVLPWVGFLCNTWLGALIGIVV
                     YIGSRLYSPEEEKILAKFFGQAWDDYCNKVLLPWI"
     gene            complement(2318..3412)
                     /locus_tag="PKZ74_11830"
     CDS             complement(2318..3412)
                     /locus_tag="PKZ74_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPM93487.1"
                     /translation="MRIIVSLFFGWMGFLPVWMFGQAALKPGENLAGIMLPAGYKIEV
                     YTDQVPNARAMDFAEDGTLFVGSMVEGKVYAVLPDRTVIIVDDSLNMPAGLDYYNGDL
                     YVSAVSRILKYEDIMQYLDEKPEPKEINGNFPDDRWHGWKFIRVGPDGKLYVPVGAPC
                     NVCLPDSVWYARILRMTLDGGGLEYYAEGVRNTVGFDWEPETRIMWFTENGRDEMGDD
                     FPPDELNKAPVPGAHYGFPFMNGKAFDPEYWPQRPRNKSFITPAMELPAHVAALGMRF
                     YTGKMFDKKYQGGIFIAEHGSWNRSKKTGYRISFITLSESRPIHYEVFASGWLKGEEV
                     WGRPADVQVGPDGSLFVSDDLAGCVYRIFK"
     gene            complement(3501..>5156)
                     /locus_tag="PKZ74_11835"
     CDS             complement(3501..>5156)
                     /locus_tag="PKZ74_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPM93488.1"
                     /translation="PSQFYVTLLYNQGLSLHMKIVRIIITAVAVISALTIQAQDQPGS
                     GTAMKGAFVDQAGSPVVLLGDTLFTIHSAYGAFSAGDRAAAISDKLMKVVKEPVFYAD
                     SLTGFYVESDYYIVYRNKVLMVIQPADTAGTGMTQDEISGRVFNLLNEELAAKSAKFN
                     ITATVTNIAYSLIVVFVLVLIIWLLNWLFRKIIKYAEKHKERLFKSIKINEYEFLTAE
                     REFVVARGLLRFFKIVLIIILFLVALPVIFSIFPSTEGITRKIIAMIWEPIQNILMGI
                     IGYLPKLITIIIIYLLFKYLIRLIRYLTKEVENKALVIKGFHPEWALPTFNIVRILLY
                     AFMFIIIFPYLPGSDSPIFQGVSVFLGLLISLGSTSLIGNTLAGLVITYMRPYKVGDR
                     ITIEDITGDVIEKTLMTTRLRTIKNEEVTIPNSKILTGYTVNFTAATLDEGLIIHTTV
                     TIGYDAPWRKVHELLIRAAEKTAGVAVQPKPFVLQTGLEDFYISYQINAYVKNSHGIV
                     RIKSDLHQNIQDEFNKAGVEIMSPHYRAERDGNKVTIPENSEE"
BASE COUNT         1311 a         1337 c         1189 g         1319 t
ORIGIN      
        1 tgcacctccg cccagggtca ctactccttc aatggtccct tcattcggat cgctcgggcc
       61 ttacgatccc aatgaaggga ccattgaagg agtagtgacc ctgggcggag gtgcaggcag
      121 tgtgacccag gtaaccgtta cggctggtga atacagcacg aatcccgata atacgggttt
      181 ctattcgatg gtggtggaag aaggcaccta cgatgtgatc ggtacgctgc agggttatta
      241 tcccgataca gtattcagcg taatggtgat caccggtgaa acaacagaag atgttgatct
      301 tacgctccag gccctgccga caggattcat cagcggcaat gtctcccttt ccgaaggcaa
      361 tgccgatctg accgaggtcg aagtctcggc gggattgcat acggtgaatc ccgatgcaaa
      421 cggcgactat atcatggaga tagagatcgg cacctacgag gtgacggctt cgctggatgg
      481 atatatgccg gaaaccgaga ccggtgttgt ggtcgtggaa ggtcagacaa cgaccggtgt
      541 cgactttatc ctcgatcctg tgcccactac aggatttatc acgggtattg tcctcctggc
      601 aggaggtgac ggagaggtga ccgaagtcaa tgttacggca ggcaatgtga ccgttaatcc
      661 cggtgaagat gggatttacc tgctcgaaat tgtggccgga acgtatgaag tgacggccag
      721 tctgcccggc tacctgaccg gggtagtacc caatgtggtg gtactggtgg aacaaacgac
      781 tcctgatatt gacttctatc tctatgaagc gcccgatatc ggttatatcg aaggatgggt
      841 aacgctgaac ggccctggcg atgtgaccca ggcggttgtg acggccggca accagtccga
      901 caatccgaat gcccagggtt attacttcct gggacttccg gcagggacat acaccgtgac
      961 tgccgatcat ccttatactt tacccgaaac cctggaagga atagaggtga tcgccgggca
     1021 gaatactgaa aatgtcaact tcgggctcga tgtcctgcgt tgtaatctgt cggtgaaagc
     1081 tgtcgaccag tacggttctc ttttatcgga tgttgaagtg gatattattg gacctgagga
     1141 tatttatacg ggaattattg agaatgattc ggttattttt gaagatgtgc cttacggatt
     1201 gtatgccggc actgcatggc tggtgatgag ccccgatacg gcccgtacca acgacgagat
     1261 caacgacagc gaccaggata tggagtttgt gtttatcctt ggcgggatga atgatgaaag
     1321 ccaaacccgg gtcaggtcat tcagcatttc accgaatccc ttctctgaag aggtcaggct
     1381 gtcgttcgaa gttgagaatc ctgtcgaggt gattctgaag gtttattctt ctacagggcg
     1441 attgataaaa atccttgccg aaggcaggtt tgatcgtggc aggcacgagg ttcagtggaa
     1501 cggtgatgag gacggaggca accgtctgcc ggcagggatt tacacggttg ttttccaggc
     1561 tggaggtgaa agggaggcta aggttttgat cctgagatag gaaagggatg gcgtccctcg
     1621 ggacctcgtc ccgaagggat ggcgtccctt tttttgagat ggagtctcat ttaaacgaga
     1681 ctccatctca atttaatgcc ttctgtttta tacctttacc taatgtttta tcaacttgaa
     1741 ggaatttgcg cttaaacaag gttgatgaaa atcaaagaga actaaaaggc aatcttcttc
     1801 taacaatctt attatctgta cattatgatg ctgtaaatcc ttgtcgggaa cggaagaaaa
     1861 atcggattgc tggccctgcc gttcatcgtt gccggtttaa ccctgaacat cctctacccc
     1921 gaaatcttta gcgtctgcgg accgacactt ggtcttatca tcgcttcggt catcatactc
     1981 ataccgggca ttatcatctg gatatggtca gtggttttga tcctgacgaa gattccccgc
     2041 agggaactga tcaccaccgg gccttatgct ctggtcaagc atccgctcta caccggggtg
     2101 gcgctactcg tcctgccctg ggtcggcttc ctgtgcaaca catggctcgg ggccctgatc
     2161 ggcattgtag tttacatcgg atcgaggctt tattcgccgg aagaagaaaa gatcctggcc
     2221 aagttcttcg gccaggcctg ggacgattat tgcaataaag ttctcctccc ctggatataa
     2281 gagaccatat cgaaaatcgt aaatcgtaaa tcgaatatca cttaaaaatc cggtaaacac
     2341 atccggctaa atcatccgaa acgaacaatg atccgtcggg cccgacctgc acatcggccg
     2401 gccttcccca aacctcctcc cccttcagcc atccgctggc aaatacttca taatggatcg
     2461 gccggctctc cgaaagggtg atgaatgaga tgcgataccc ggtctttttc gaccggttcc
     2521 atgaaccgtg ctcggctatg aaaatccctc cctggtattt cttgtcaaac attttcccgg
     2581 tgtagaatct catccctaaa gcagcaacat gagccggtag ctccatcgcc ggcgtaatga
     2641 aactcttatt ccgcggccgc tgcggccagt actccggatc gaaagctttg ccgttcataa
     2701 atggaaatcc gtagtgcgcc ccgggtaccg gcgctttgtt cagttcgtcc ggcggaaaat
     2761 catcccccat ctcatccctg ccgttttcgg taaaccacat gatcctcgtt tcaggctccc
     2821 agtcgaatcc taccgtattc ctcacccctt ccgcatagta ttccaggcct ccaccgtcca
     2881 gcgtcatcct taatattctc gcataccaga cactgtcggg caaacaaaca ttgcacggcg
     2941 cccccaccgg tacatacaac ttgccatcgg gcccaacccg gatgaacttc catccatgcc
     3001 agcggtcgtc cgggaaatta ccattgatct ctttcggttc cggcttctcg tcaaggtatt
     3061 gcataatatc ctcatacttc agtatcctgg aaacagccga aacataaagg tctccgttgt
     3121 aataatcgag ccccgccggc atattcagac tgtcgtctac aatgatgacc gtacgatccg
     3181 gcagtacagc gtaaaccttt ccctctacca tcgaaccgac gaacagcgtc ccgtcttccg
     3241 caaaatccat ggcacgggca ttcggcacct ggtcggtgta aacctctatt ttgtaacccg
     3301 ccggcaacat gatcccggca agattttcac ccggtttcag ggccgcctgt ccaaacatcc
     3361 acacaggcaa aaaccccatc catccaaaaa ataaactaac tattatcctc atatcctgaa
     3421 taaattctaa tcatcccaaa atcaaatccc aaaaccattc ccttttcact cttcactttt
     3481 cactcttcac ttttcacttt tcactcttca ctattctccg gaatcgtcac cttattccca
     3541 tcccgctcgg cgcgataatg cggcgacatg atctcaaccc ccgccttgtt gaactcgtcc
     3601 tgtatgttct gatgcaagtc cgatttgatt cttacgatgc catgagagtt tttaacataa
     3661 gcgttgatct ggtagctgat ataaaaatcc tccagcccgg tctgcaaaac aaaaggtttc
     3721 ggttgaacag ccacccccgc cgtcttttcc gcagcccgga tcagaagctc atgcaccttc
     3781 cgccacgggg catcgtagcc aatcgtcacg gtcgtgtgaa tgataagacc ctcatccagc
     3841 gttgccgctg tgaaattaac cgtataaccg gtcaatatct ttgaattggg aattgtaact
     3901 tcttcattct taattgttct gagccttgtc gtcatcaggg tcttttcaat aacatcaccc
     3961 gtaatatctt cgatggtgat cctgtcgccc actttataag gcctcatgta agtgatcacc
     4021 agcccggcca gggtgtttcc gatcagtgaa gtggaaccca gcgaaataag caatcccagg
     4081 aaaaccgata ccccctggaa tatcggggaa tcggaaccgg gaaggtatgg gaatatgatg
     4141 atgaacatga aagcatagag caggatgcgg acgatattaa aagtcggcag ggcccattcg
     4201 ggatgaaatc cttttattac caatgcttta ttttcaactt ccttcgtaag atacctgata
     4261 agccggatca ggtatttgaa taataagtag ataataatga tggttatcaa ttttggaaga
     4321 taaccgatga tccccatgag gatattttgt atcggctccc agatcatggc gataatcttt
     4381 cgggtaatcc cctcggttga tgggaaaatg ctgaaaatca ccggcaaggc taccaggaac
     4441 aggattatta ttaaaacaat cttaaaaaac cgcaacaatc ccctggctac cacaaactcc
     4501 cgctccgccg taagaaactc atattcattt attttgatgc ttttgaaaag acgttcttta
     4561 tgcttctctg catattttat tatcttcctg aataaccagt ttaataacca gatgatcaat
     4621 accaggacaa agaccacaat cagcgagtaa gcaatgttag taacagttgc cgtgatgttg
     4681 aatttcgcgc tcttcgccgc cagttcctca ttcagcaaat tgaacaccct acctgaaatt
     4741 tcatcctgtg tcatacctgt ccccgcggta tccgccggct gaataaccat cagcaccttg
     4801 ttgcggtaaa caatgtaata atcgctttca acatagaaac cggtcagcga atcagcataa
     4861 aaaacaggct ccttcaccac cttcatcagc ttatccgata tggcagccgc gcggtctccg
     4921 gctgaaaaag cgccataggc agaatggatt gtgaacaatg tatctccaag aagtacaacc
     4981 ggactaccgg cctggtccac aaaggcaccc ttcatggcgg ttccgctgcc cggctgatcc
     5041 tgtgcttgaa ttgtcaaagc tgatattacg gcaaccgcag ttatgattat cctgactatc
     5101 ttcatatgta aactaagccc ttggttatat aacaaagtta cataaaattg agatgg
//
LOCUS       DAOPNH010000251         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_18926_length_5118_cov_5.702153, whole genome shotgun sequence.
ACCESSION   DAOPNH010000251 DAOPNH010000000
VERSION     DAOPNH010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18926_length_5118_cov_5.702153"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="PKZ74_11840"
     CDS             complement(<1..2223)
                     /locus_tag="PKZ74_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93489.1"
                     /translation="MKKNDRNIKISLFLVFFLVMGLYWSSNAQVGQSSDFVDPYSIWL
                     NPGTDDFATIQQNVESYYANIDRSKKGTGYKQWKRWEYIQQNRLSPDGKIINYAARNL
                     EEFDTYYKGSGSREITATTYGYWGSLGADYFVDGNGWNGGIGRVNCITFHPSVANTFW
                     VGTPSGGLWRTTTGGTSWTPLTDGMPRIGVSGLAVNYNNTNIMYLLTGDGDGGDVSSI
                     GVMKTTDGGVTWYPTGLTWAATDFERGYKILMHPTNPAILFVVSTAGIFRTANSGTTW
                     TNVHSTAGSNYHDIEFKPGDPSIVYACAGSKFYRSLDGGVTWTQITSGVPTTATRMAI
                     GVSPNNDAYVYLFAGPSYTSGVFVGMYLSTSSGSTFSTRSTTPNILGYSSTGADDDDQ
                     TTYDHAIAVSRTDVADVIIGGINTWSSTNWGATWTLTSMWNNAPGPDYTHADIHALEI
                     NPLNNYLYCGSDGGIFRSTDFGANWTDLSDGLEITQSYRIAGYEPNEDLITNGTQDNG
                     SNKWSGGTTFLHMLGADGMDCMIDHSNPNNLYNCIQFGNIYKSTNGGSTFSFIAPLVD
                     AGPWVTPLAMSPANSQILYAGYSDIYKSTNGGASWTNIGYSGTGAIAVGTSNSSRVYA
                     SVGGTKTIYMSDDAGATFTDVSAGLPSGSITFIAVNPDNSFDVFVTYGGYTSGQKVYR
                     SVNAGTTWTNISGTLPNIPVNCIAYQDTDGAPNDAIYIGTDLGVYYRDDEIGDWIPFM
                     N"
     gene            complement(2242..2631)
                     /locus_tag="PKZ74_11845"
     CDS             complement(2242..2631)
                     /locus_tag="PKZ74_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93490.1"
                     /translation="MKTIKYMFLFLFVCVFADVFAQQENKTEGITKTGTGEGSPALTN
                     TPANRPPEGHIIPSVVTPALNGTVQELVKNDLNRVDEPNAASPDPKATSSGSVFEGEI
                     KDRETGIKEDNEPNAASPPKGDIPEIQ"
     gene            complement(2699..3679)
                     /locus_tag="PKZ74_11850"
     CDS             complement(2699..3679)
                     /locus_tag="PKZ74_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93491.1"
                     /translation="MKSLKITFGFFALVSFLGLNEAVGQWAPNGSHIYNTNAGNVGVG
                     YNTPASLLHVGKNMTEPTIRVQNFGGAGGATFQMIDNASTADWKFKATNTGGFKIRDN
                     AFAMDVITVEPNSLANAFYIDAAGFIGMGTNAPSDNLHIISDSYPFIFVEGTLAGGNQ
                     GIAFRENSVTRGWIYYSGSADGVRINAHSSGGSRNDIFIKSDGRVCIGTTTAATGYAL
                     SVNGKAACTEVLVDNTADWPDYVFSDEYDLMSLEELEQSIKENNHLPGIPSATEVEEN
                     GILLGEMQKNLLEKVEELTLYTIMQDKQIKELQMKIEALEKSKSGKGKNR"
     gene            complement(4005..>5118)
                     /locus_tag="PKZ74_11855"
     CDS             complement(4005..>5118)
                     /locus_tag="PKZ74_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93492.1"
                     /translation="GSNKWTGGATMTHTLGADGMDCMIDHSNQNTLYNNIQFGNLHKS
                     TDGGASFAFIAPLVDAGPWVTPFVMQVNNAQVIYGGYYDIYKSINGGASWSNLGYNGS
                     GAMALGGSSNPGRVYASVEGSRTIYMSNDYGASFTDVTSNLPSGSITFIAVNPGYSGD
                     VWVTYGGYSAANKVFRSTDAGTTWTNVTGSLPNIPVNCIAYQETGGTPNDALYIGTDV
                     GVYYRDDDIGDWVPFMNGLPAVMVFDLEINESAGVITAATYGRGFWRSELYSSCPTSY
                     FLDQANDPGNPNYTGFQHYEASSTVISSRIITGGIGTDVTYQAGTYVLLETGFHARAG
                     NKFQAVLGPCSGTAPPAASPDRVLKVTGTYSGQKID"
BASE COUNT         1303 a         1250 c         1188 g         1377 t
ORIGIN      
        1 gttcatgaac gggatccaat cgccaatctc atcatcacga taatacacac ccagatccgt
       61 gcctatgtaa attgcatcat tcggagctcc gtctgtgtcc tggtaagcaa tacagttcac
      121 cggaatattc ggcagagtgc cggagatgtt cgtccaggtt gtgccggcat ttacagaacg
      181 gtaaaccttc tggccgctgg tataaccacc atacgtaacg aaaacatcaa atgaattgtc
      241 gggattgaca gcgatgaacg tgatggagcc tgaaggcagc cccgccgaaa catcggtaaa
      301 tgttgccccg gcatcatccg acatatagat cgttttcgtg ccgcccaccg aagcataaac
      361 tctgcttgaa ttactggtgc cgacagcaat ggcgccggtc ccgctatagc cgatattggt
      421 ccacgatgcg ccgccgttgg tgcttttgta aatatcgctg tagccggcgt aaaggatctg
      481 tgaatttgcc gggctcatag ccagcggggt cacccagggg cccgcatcca ccaggggagc
      541 gatgaatgaa aatgtgctcc cgccgttggt ggatttgtaa atattgccaa actggataca
      601 attatacaag ttgttcggat tggaatgatc aatcatgcaa tccataccat cggcgccaag
      661 catatgaagg aaagttgtcc ctcccgacca cttgttggaa ccgttatcct gggtgccgtt
      721 ggtgatcagg tcctcatttg gctcataacc ggcaatccgg tatgattggg tgatctcgag
      781 gccatccgac aggtcggtcc agtttgctcc gaaatcggtc gaccggaata tccctccgtc
      841 acttccgcaa tagagatagt tattcaacgg gttgatctca agtgcatgaa tatcggcgtg
      901 tgtatagtca ggaccgggag cattgttcca catcgacgtc aaagtccagg tagcccccca
      961 gtttgtcgaa ctccaggtat tgatccctcc aatgattacg tccgcgacat cggtccttga
     1021 aacggcgatg gcatgatcat aggtagtctg gtcatcatcg tcggcccctg tggaactgta
     1081 accgagtata ttcggtgtgg tcgagcgggt tgagaaagta gatccactgg aggtggaaag
     1141 gtacattccg acaaaaacac cgctggtata agacggaccg gcaaacaggt aaacataggc
     1201 atcattattg ggggaaaccc cgatggccat cctggttgca gtggtcggaa ccccgctggt
     1261 gatttgagtc caggtaacac caccatccag cgaccggtaa aatttcgacc cggcacaggc
     1321 gtagacgatc gacggatcac ccggcttgaa ttcaatatcg tgataattgc tgccggcggt
     1381 tgaatgcaca ttggtccatg tagtacccga gtttgctgtt ctgaaaatac ctgctgtgct
     1441 cacaacaaat aagatcgccg ggttggtggg gtgcatcagg atcttatatc ctctctcgaa
     1501 atcggttgca gcccatgtca aaccagtcgg ataccatgtt accccaccgt cggtagtctt
     1561 catgacgccg atagagctga catctcctcc atcgccatca ccggtaagga gatacatgat
     1621 attggtgttg ttgtaattaa ccgccaaacc gctcacgccg attctcggca tcccgtcggt
     1681 cagcggtgtc cagcttgtac ctccggtggt ggttctccaa agcccgccgg atggtgtccc
     1741 cacccagaaa gtattggcga cggaaggatg aaatgtgatg cagttcaccc gtccgattcc
     1801 cccgttccag ccattgccat cgacaaagta gtcggctccg agagaacccc agtaaccata
     1861 agtggttgcc gtaatctccc ttgaaccgct tcctttataa tacgtatcga attcctccag
     1921 attccgggct gcatagttga tgatctttcc atcgggagaa agacggttct gctgaatgta
     1981 ttcccagcgt ttccattgct tgtatccggt cccctttttg gatcgatcaa tattggcata
     2041 atagctttcg acattttgct gaatggttgc aaaatcatcg gtaccagggt tcagccagat
     2101 tgagtatgga tcgacaaaat cggaggattg gccgacctgc gcatttgaac tccagtataa
     2161 tcccattacc aggaaaaata ccagaaatag acttattttg atgtttctgt cattcttttt
     2221 catagtcctg atttttaaga attattggat ttcggggata tcaccttttg gcggacttgc
     2281 cgcgttcggt tcgttgtctt cctttatgcc ggtttctctg tcttttattt caccttcgaa
     2341 cacagacccg gatgaagttg ctttcggatc cgggctggcc gcattcggtt catcaacccg
     2401 gtttaaatcg ttctttacga gctcctgtac tgttccattg agggcaggag ttacaacgga
     2461 tggaatgata tggccttcag gaggccgatt tgccggcgta ttggtcaatg cgggtgaacc
     2521 ttctcctgtc cctgttttag taattccttc tgtcttattt tcctgctggg caaacacatc
     2581 tgcaaaaaca cagacaaata agaaaaggaa catatatttt attgttttca tattcttcag
     2641 attaaaattt ataatgagga taatactaac cttcctgtac taaatatttc agggataatt
     2701 agcggttttt accttttcct gatttgctct tttccagagc ttcaattttc atctgaagtt
     2761 ctttgatctg tttatcctgc attatcgtat aaagagtaag ctcctcaact ttctccagga
     2821 ggtttttctg catttcaccc agtagaattc cattttcttc aacttcagtg gctgagggga
     2881 ttccgggaag gtggttgttt tctttaatgc tctgctcaag ttcttcaagg ctcatcagat
     2941 catactcatc agagaaaaca taatcgggcc aatcggctgt gttatctacc aatacctcgg
     3001 tacatgcggc ttttccattg accgacaaag catatccggt cgcagcagta gtcgttccga
     3061 tacaaaccct gccatcgctc ttgatgaaaa tatcattacg cgatccgccg ctggaatgtg
     3121 cgtttattct cacaccgtca gcagaaccac tgtagtaaat ccatcctctt gttacgctat
     3181 tttccctaaa ggcaattccc tgatttcctc cggctagtgt accctcgacg aaaataaacg
     3241 gataagaatc gctgatgata tgaagattat cggaaggggc gtttgtaccc atgccaataa
     3301 aacccgctgc atcgatataa aatgcattgg ccaggctgtt cggttcaacg gtgatcacat
     3361 ccatggcaaa agcattatcg cggatcttaa aaccgccggt attggtggct ttgaatttcc
     3421 agtcggctgt actggcattg tcgatcatct gaaaggttgc gccaccggct ccgccaaaat
     3481 tctgcacacg gatcgtgggc tcggtcatat tcttaccaac atgaagaagg gaggccggag
     3541 tgttatatcc gacacctaca ttaccggcat tggtattgta aatgtgtgaa ccgttcggag
     3601 cccattgtcc gactgcttca tttaatccca ggaatgagac taaagcaaaa aatccaaaag
     3661 taatttttaa agatttcata atcaattgtt tttagtttat aaattattat aaagaagtta
     3721 ttaatgacag attaatggtc agggtttgat caaaggtagg tatggatata tatccatatc
     3781 aaatttttaa caattactta agaattttaa ggaaaaatta agatatgaaa aaagaggttg
     3841 cccgaagcca tgagcaacct cctttttcgt taatagtccg acgactaaag ccgtcggttt
     3901 ttttgaaaga tttccttccg acgactaaag ccgtcggttc cggtttttta atttacttgg
     3961 aaccactggc ttgagtcgtt ggagtgagtt tacaaacaaa tatatcaatc gatcttctga
     4021 ccggaataag tcccggtgac cttcaacacc ctgtcggggg aagcggccgg tggcgcagta
     4081 ccggaacagg gcccgagaac agcctggaat ttgttcccgg ctctggcatg gaagcccgtt
     4141 tcaagcagaa catacgtccc ggcttgataa gtcacatctg tcccgatacc tcccgtgata
     4201 attcggctgg agatgacagt actgctggcc tcataatgct gaaaaccagt ataattcgga
     4261 ttacccggat cattagcctg gtccaggaag tacgaggtcg ggcagctcga atagagttcc
     4321 gaacgccaga acccacgtcc gtatgttgct gcagtaataa ctccggctga ttcattgatt
     4381 tccagatcaa ataccattac ggccggaagc ccattcataa acggaaccca gtcgccgata
     4441 tcgtcatccc tgtaataaac accgacatcg gtaccgatgt agagcgcatc gtttggcgtg
     4501 ccaccggttt cctggtaagc gatgcaattg acaggtatat ttggcaggga gccggtaaca
     4561 ttggtccagg tggtgccggc atctgtggaa cgaaacacct tattcgctgc agaatatcca
     4621 ccataggtaa cccaaacatc tcctgaatag cccggattga ctgcaataaa tgttatggaa
     4681 cccgaaggca gattgctcgt cacatcggta aaagaagcgc cgtaatcatt tgacatataa
     4741 attgtacgag atccttcaac agaggcatag acccgaccgg gattactgct gcctcccagt
     4801 gccattgcac ccgaaccatt atagccgagg tttgaccagg aagctccccc atttatactt
     4861 ttgtagatat cgtaataacc cccataaatc acctgtgcgt tgttcacctg catcacaaac
     4921 ggggttaccc atggccctgc atcgaccagc ggagcgataa aggcaaaaga tgccccacca
     4981 tccgtcgatt tgtgcaggtt cccgaactgg atattattat aaagggtatt ctggttgcta
     5041 tgatcgatca tacaatccat cccatcagcg ccgagggtgt gggtcattgt ggccccgccg
     5101 gtccatttgt tggaaccg
//
LOCUS       DAOPNH010000252         5089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19076_length_5089_cov_6.472388, whole genome shotgun sequence.
ACCESSION   DAOPNH010000252 DAOPNH010000000
VERSION     DAOPNH010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19076_length_5089_cov_6.472388"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKZ74_11860"
     CDS             complement(<1..476)
                     /locus_tag="PKZ74_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM93493.1"
                     /translation="MPFSNIAYRTPKYYFSDTSFNKLMRRRIYHVLLISSTYDAFVLE
                     DDGRIDEQIFIEYVSLNLRYPPQFILADSEKKAFDILHDEKIDLVVVMLSSEEMDTFS
                     LAEKIKMKNPAIPIVVLTPFSREVTLKLNDTTKSAVDYIFSWLGNADLLLAIIKLI"
     gene            complement(527..1078)
                     /locus_tag="PKZ74_11865"
     CDS             complement(527..1078)
                     /locus_tag="PKZ74_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPM93494.1"
                     /translation="MKSMNVKSLLALFITFIIFTACSSSMKSLQTVPKVDLERYSGKW
                     YEIARLPNSFEKGLICCTAEYSLRDDGRITVINTGYSEKDPSKISSVKGKAWNPDKNE
                     PGRLKVQFFWPFSGGYYIFHLDPEYRYVLVGSPSRKFFWLLARNPVIEENLYGELVRI
                     AADNGFATQNLIRVSQECHKQAK"
     gene            complement(1091..2338)
                     /locus_tag="PKZ74_11870"
     CDS             complement(1091..2338)
                     /locus_tag="PKZ74_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPM93495.1"
                     /translation="MEAEIISIGDELLIGQVINTNASWMARRLAENGIRARNMVVVPD
                     EAEDIRKAVDVAFGRSEVILVTGGLGPTKDDITKKVLCDYFGTRLVFHQPSFDNILRL
                     FPTRASNLSPANRQQADIPENCTPVMNQFGTAPGMIFRKEGRILVSMPGVPFEMEAMM
                     NNDVIPMLKKSANSDVMVCKTLLTQGVGESYLSELIAVWEESLPENMKLAYLPQPGIV
                     RLRLNAYGDNRETLQEQIDFQSAKLEEIIPDLIFGYGDDTMEAVAGKLLREKGKSLST
                     AESCTGGYLAHLITSVPGSSDYFKGSVVAYANDIKMALLGVSEGSLARHGAVSEEVVR
                     EMALGALEKFNTDYAVSTSGVAGPDGGTPEKPVGTVWVAVASRIGATTKLFRLGDHRG
                     RNIRRAALSALNMLRLELLKTKS"
     gene            2480..3328
                     /locus_tag="PKZ74_11875"
     CDS             2480..3328
                     /locus_tag="PKZ74_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPM93496.1"
                     /translation="MIVNSDGSIYHLKLRPEEIADLVLVSGDPARIGMISDHFDTVIF
                     RRQNREFVSHLGTYNGARILALSTGIGPDNIDIVMNELDALANIDLVSREILPEKRSL
                     TIIRIGTSGTIHADIPVGSFVVSSHGLGMDGSLNYYAGLKEVTDSELTQAFARQTAWP
                     AHLPVPYIIPGSSDLVEKLSGAGITGITATAGGFYGPQGRQIRLNSSFPEMLPALARF
                     QHNGHRIINFEMETSSLYGLGRMLGHRAASICVLLANRATGHYTRNIRQDEERLIGYV
                     LDKMTS"
     gene            3380..4252
                     /locus_tag="PKZ74_11880"
     CDS             3380..4252
                     /locus_tag="PKZ74_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPM93497.1"
                     /translation="MAKNGNKELEALISLLDEPDEDIFLTIRDRIFAYGLDAVPFLES
                     VWENAFDPLVQHRIEEIIHNLQFEDLKREMMQWARFGYHDLLQGALLVSKFQYPDLKE
                     DEIIKKVGQFSQDIWLELNQNLTGLEKVKVINHILFDTHKLAGNLANVNSADNYFLNS
                     LLDTKKGSPLLLGIVYISIAQSLKIPVYGVDLPRHFVLAYTDEIILAPRDIPEGDVMF
                     YINPFNKGAVFTKNEIDLFIKQLKLERKESYFTPCDNKTIIRRMMNELIYIYDQSGNP
                     VKKDEMTELMGMMD"
     gene            complement(4265..>5089)
                     /locus_tag="PKZ74_11885"
     CDS             complement(4265..>5089)
                     /locus_tag="PKZ74_11885"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM93498.1"
                     /translation="DRISAFDVVLPKGIPYKGQVLNQIAAKFLDATRDIVPNWMMAVP
                     DPMVMVGKFCEPFPVEMIIRGYLTGSSWRTYKSGAREICGVPIPDGMKEHERFPEPIV
                     TPTTKAKEGHDEDISREEIIRQGLVSEEDYNLLEKYTKELFRRGSEIAASMGLILVDT
                     KYEFGKHNGQIYLIDEIHTPDSSRYFYADGYEERFARGEQQKQLSKEFVREWLMANGF
                     QGKEGQAVPEMTPEFVESVSERYIELYEGITGEKFVRADAREVVKRVEENIRDFLK"
BASE COUNT         1173 a         1395 c         1294 g         1227 t
ORIGIN      
        1 tcgatcagct taatgatggc cagcagaaga tctgcatttc cgagccagct gaagatataa
       61 tcgacagcgc ttttcgtcgt atcgttcagt ttgagggtca cttcgcgtga gaagggtgta
      121 aggacaacga tcgggatcgc cgggtttttc atcttgatct tctcggcaag gctgaatgta
      181 tccatctctt cggagctgag catgaccacc accaggtcga tcttctcgtc gtgcaggatg
      241 tcgaacgctt tcttttccga atcggccagg atgaactgcg gcggatagcg aaggttcagt
      301 gagacatatt cgatgaaaat ctgctcgtcg atccggccgt cgtcttccag cacaaaggca
      361 tcgtaagtgc ttgatatcag caggacatga tagatcctcc gccgcatcag cttgttgaat
      421 gaggtatcgc tgaaataata ttttggcgtc cggtaggcta tgttggaaaa aggcatcgga
      481 ataatgttga attcttaatt cttaatgcta aactttggaa tttgaactat tttgcctgtt
      541 tgtggcattc ctggctcacc ctgatcaggt tctgtgttgc aaatccgtta tctgcagcga
      601 tccggaccag ttcgccataa aggttctcct caatgaccgg attacgggcc agcagccaga
      661 agaatttccg ggacggactc ccgacaagga cataccggta ttccggatcg aggtgaaaga
      721 tgtagtaccc gccgctgaat ggccagaaga actgcacctt cagcctgccc ggctcatttt
      781 tatcagggtt ccacgctttg cccttcaccg agcttatttt cgacgggtct ttttcactat
      841 aaccggtatt gatcaccgtg atccggccgt cgtcgcgcag gctgtattcc gccgtgcagc
      901 atatcagccc tttctcgaag gaattcggca agcgggctat ttcgtaccat tttcccgaat
      961 atctctccag gtcaaccttt gggacagttt gcagtgattt catagaagat gagcaagcag
     1021 taaagataat gaaagtgatg aacaacgcca aaagggattt cacattcatg gatttcatgg
     1081 cattttggtt ttaagatttc gttttcaaca attccagcct gagcatattc agggccgaca
     1141 acgcggcgcg gcggatattg cggcccctgt ggtcgccgag ccggaagagc tttgtagtgg
     1201 ctccaatacg ggaggccact gcaacccaaa cagtaccgac cggtttttcc ggcgtgcccc
     1261 cgtcaggacc cgccactccg gatgtggaaa ccgcgtaatc ggtattgaac ttttcaagcg
     1321 cccccagcgc catctccctg accacctctt cgctgacggc cccgtgcctt gccagcgatc
     1381 cttccgaaac gccgagcaag gccatcttga tatcattggc ataggcaacg accgagccct
     1441 tgaagtaatc cgatgagccc ggcacgctcg tgatcagatg cgcgagatag cccccggtac
     1501 agctttcagc cgtgctcagc gattttcctt tctccctgag caatttccct gctacagctt
     1561 ccatcgtatc atcgccatat ccgaaaatca gatcggggat gatctcctca agctttgcgg
     1621 attgaaagtc aatttgctcc tgcaatgttt ccctgttatc cccgtaggca ttcagcctga
     1681 gcctgacaat accgggctgc ggcaggtagg ccagcttcat gttctccggc aggctctctt
     1741 cccagacagc gatcagctcc gacaggtagg attcgccaac gccctgcgtc agcagggtct
     1801 tgcacaccat tacatcgcta ttggcgcttt ttttcaacat cgggatgaca tcgttgttca
     1861 tcatggcctc catttcgaaa gggactccgg gcatcgacac caggatccgc ccttctttcc
     1921 tgaagatcat tcccggagcc gtaccgaatt ggttcatcac cggggtgcag ttctcaggga
     1981 tatcggcctg ctgccggttg gcggggctca ggttgctcgc ccgggtgggg aagagcctca
     2041 ggatgttatc gaaggagggc tgatgaaaca ccagccgggt tccaaaataa tcgcacaaaa
     2101 ctttcttggt gatatcatcc tttgtcgggc cgaggcctcc cgtcaccagg atcacttcgc
     2161 tccggccgaa ggccacatcc accgctttcc ggatatcttc cgcctcatcc ggaaccacaa
     2221 ccatattcct ggcccggatt ccgttctccg ccagtcgccg tgccatccac gacgcgttgg
     2281 tattgattac ctgcccgatg aggagttcat ctcctatact gataatttcc gcttccatgg
     2341 tgtaaaatta atatctcccg ggaatggtaa tgtaacgggg ggaattttaa ttttgatgtc
     2401 ctttacataa ggtgattttt ttcaccacgg agtacacaga gttacacaga ggagagacag
     2461 agttatgaaa gaatcggatt tgatcgttaa ttcagacgga agcatatatc acctgaaact
     2521 gaggccggaa gagatcgcgg acctggtgct ggtatcgggc gatccggccc gcatcggcat
     2581 gatttcagac catttcgaca cggtgatctt caggcggcag aaccgggaat ttgtgagcca
     2641 ccttggaacg tacaacggcg cccggatcct ggccctatcg accggcattg gcccggataa
     2701 tatcgacatt gtcatgaacg aactcgatgc gctggccaat atcgacctgg tttccaggga
     2761 aatattgcct gaaaaacgtt ccctgacgat catccggatc ggcacctcgg gcacgatcca
     2821 cgccgatatt ccggtcggtt cctttgtggt ctcttcccac gggctgggca tggacggttc
     2881 gctgaactat tacgcaggtt taaaagaagt caccgacagc gaattgaccc aagcgtttgc
     2941 caggcagacc gcatggccgg cgcatttacc ggtgccctac attatcccgg gcagcagcga
     3001 cctggtggaa aagctttcgg gcgccgggat cacaggcatt acggcaaccg cgggcggttt
     3061 ttacggcccc caggggcgtc agatcaggct gaacagctcg tttccggaga tgctgccggc
     3121 attggcccgg tttcagcata acggtcaccg gatcatcaac ttcgagatgg aaacctcgtc
     3181 gctgtacggc ctgggccgga tgctcggcca ccgggcagcc agcatctgcg tattgctggc
     3241 caacagggct acagggcact acacgcggaa tatacgccag gatgaggagc gcctgatcgg
     3301 atatgtcctc gacaaaatga caagctgaac caacaaggca ttatctttgt tacagggata
     3361 ttgacaattg cggtattaca tggcaaaaaa cggaaataag gaactggagg cgctgatcag
     3421 tcttctcgat gaaccggatg aggatatctt cctgacgatc cgcgaccgca ttttcgcata
     3481 cgggctcgat gcggtgcctt tcctggagag cgtctgggag aatgcgtttg acccgttggt
     3541 ccagcaccgg attgaggaaa tcattcataa cctgcaattt gaagacctga agagagagat
     3601 gatgcaatgg gcccggttcg gctatcacga tctcttgcag ggcgcactgc tggtcagcaa
     3661 attccagtat cctgatctga aggaagatga gatcatcaag aaggtcggcc agttctcgca
     3721 ggatatctgg ctcgaactga accaaaacct caccggactc gaaaaagtca aggtgatcaa
     3781 ccacattctt ttcgacacgc acaaactggc gggcaacctg gccaatgtca actcggccga
     3841 taattacttc ctcaactcgc tgctggacac gaaaaaaggc agcccattgc tgctggggat
     3901 cgtttacatc tccattgccc agagcctgaa gatcccggtt tacggtgtcg acttaccgcg
     3961 gcatttcgtg ctggcttata ccgatgagat catactggcc ccgcgcgaca ttcccgaagg
     4021 cgacgtgatg ttctacatca atcctttcaa caagggagcg gtttttacga aaaatgagat
     4081 cgacctgttc atcaaacagc ttaaactgga gagaaaagaa tcctatttca ccccctgcga
     4141 caacaaaacc atcatccggc ggatgatgaa cgaattgatc tatatctacg accagtcggg
     4201 caatccggtg aaaaaagatg agatgaccga attgatgggg atgatggatt gagagaataa
     4261 gcagttactt caggaagtcc ctgatattct cctccaccct cttcaccacc tcgcgggcat
     4321 cggccctgac aaacttttca ccggtaatcc cttcgtagag ttcaatgtac ctttcggaaa
     4381 ccgattcgac aaattccggg gtcatctcag ggaccgcctg tccctcctta ccctggaaac
     4441 cgttcgccat cagccattcc ctgacaaatt ccttggatag ctgcttttgc tgctctcccc
     4501 gggcaaagcg ctcctcgtac ccatcggcat agaaatacct tgacgaatcg ggcgtatgga
     4561 tctcgtcgat caggtagatc tgcccgttat gcttcccgaa ttcgtacttg gtatcgacca
     4621 ggatcaggcc catggacgcg gcaatctccg atccccgcct gaaaagctct tttgtgtact
     4681 tctcgagcag gttgtaatct tcttccgata ccaggccctg ccggatgatc tcctcccgcg
     4741 aaatatcctc gtcgtgccct tctttggctt tagtcgtcgg ggtgacgatc ggctcgggga
     4801 agcgctcatg ctctttcatg ccatcgggga tcggcacacc gcagatctcc ctggcgccgg
     4861 atttatatgt tctccatgaa ctgccggtga ggtacccgcg gatgatcatc tccaccggga
     4921 aaggctcgca gaatttgccc accatcacca tcggatcggg aacggccatc atccagttcg
     4981 gaacgatatc ccgcgtcgca tccaggaatt tcgcggctat ctggttcaat acctggcctt
     5041 tgtaaggaat gcctttgggg agtacgacat cgaaggccga gatccggtc
//
LOCUS       DAOPNH010000253         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19394_length_5027_cov_4.809533, whole genome shotgun sequence.
ACCESSION   DAOPNH010000253 DAOPNH010000000
VERSION     DAOPNH010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19394_length_5027_cov_4.809533"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKZ74_11890"
     CDS             complement(<1..237)
                     /locus_tag="PKZ74_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93499.1"
                     /translation="MKGRFFLFTLALLLSGLIYGQTVPRDKVIVEIGTGTWCPYCPGA
                     AMGADDLIANGHEVAIIEYHNGDDYTNIYSNARNT"
     gene            complement(247..1941)
                     /locus_tag="PKZ74_11895"
     CDS             complement(247..1941)
                     /locus_tag="PKZ74_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93500.1"
                     /translation="MKTIYLALFAFSFLVFSVHSQSQRMVLIEEATNASCGPCASQNP
                     AFDNLLNQNRDILTAIKYHWYFPGYDPMHNHNTVENNARVSYYGINGVPTATIDGDIP
                     NGPTFSYPGGPHGYTQELLDEYAAIPSPFNLWLSHRISDDEDSIYVDMMIEATQAVSG
                     TLIAHMVVVEKHINFTTPPGSNGEKNFHDVMKKMIPDQFGTTLPASFQPGEYLILQGS
                     WELANVYKIEELGVVGFIQNNVNKDVLQAANSTAEPFTPLYDHEASITKVSNVSETNC
                     LGTVKPELTIRNNGAEPLTQVDVYYRINDETTYLYEWTGNLGFLGSDVITLPEISFNI
                     IDENSLYAYTVDPNGVPDEYIGNDTIIQSFSRAEITPLTVKLMIRTDENPDETTWEIR
                     NSAGELQFSGGPYSQPNTVIQETFQMEDMECYIFRIFDEGGNGLNIPGFYALYHGSNS
                     YISNGTDFGAIDTAYFEVNTQVGIDEMQSNPPMVIFPNPARDKFTTTLWLDGQKEVRI
                     FIYDIAGQLVTSVYAGQLMAGYQTVEINTKDLAPGLYFVRTNIGPSHQTQKITIIK"
     gene            2041..2772
                     /locus_tag="PKZ74_11900"
     CDS             2041..2772
                     /locus_tag="PKZ74_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93501.1"
                     /translation="MKKILLSLFAVCFLLSANTQSFVLSYEGNTLAPDAVLVVADDPT
                     VEVIEAWVNITNNAATAKSVLVKKVIHDGDTLAGSDNTFCWGLCYPNTTYVSPFPQTI
                     QPGATSDLFYGDYRPNNLIGISKITYVFWDEMNVNDSVAVNVHYKASPASVNDLAAKI
                     MASGVYPNPASGMAYIDYSIPNSGGNNRLVITNMLGARIKEIGLKNISGKATLDVSEL
                     MNGIYFYSIISDNQQVITGKFLVRH"
     gene            complement(2847..3038)
                     /locus_tag="PKZ74_11905"
     CDS             complement(2847..3038)
                     /locus_tag="PKZ74_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPM93502.1"
                     /translation="MNTGTVKFFNNSKGFGFIKDDASDKEYFVHVSGLKDEIKENDRV
                     TFDLQEGRKGLNAVNVKKS"
     gene            complement(3101..3655)
                     /locus_tag="PKZ74_11910"
     CDS             complement(3101..3655)
                     /locus_tag="PKZ74_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPM93503.1"
                     /translation="MLSTFDIELLRFIHHGRIAGLDEALYILSYSASFVSIGIVIVFL
                     VIAVKKKTWKHRRDFLKMMAALVAAALISLVLKTLIARERPFITYPDIEKLSSGGSAS
                     FPSGHTLEAFAIAMALTLVTRRKSIAVAVFTWAFLVAYSRMALGVHYPSDVAGGVFIG
                     LFTGWFFCRLLNNKRTGRQEIKKD"
     gene            complement(3659..4777)
                     /gene="lpxB"
                     /locus_tag="PKZ74_11915"
     CDS             complement(3659..4777)
                     /gene="lpxB"
                     /locus_tag="PKZ74_11915"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPM93504.1"
                     /translation="MKYYIIAGEASGDLHGSRLISEIRNNDPGAVFRVWGGDMMEQAG
                     GHLVKHYRDLAFMGFLEVAVNLKTILKNLNYCKKDINDFRPDLLILVDYPGFNLRMAK
                     YASRKGFKVVYYISPQVWAWKKSRVYSIRKYVDKMLVILPFEKAFYSKYQFDVDFVGH
                     PLMDILNEAKDSGSRETFLKKNELHDMPVIALLPGSRKQEIKRILPVMIRAAERYPGY
                     QCVVAGAPSLDREYYRSVTGDKDVRILFGQTHDLLRHSHAALVASGTATLEAALTGTP
                     QVVCYRGSAISYAIARRLVDVKYISLVNLIMDREVVTELIQNDLTADKLEAEMQKIIQ
                     GPRRTEMISGYHSLRERLGGPGAAARAAAIIHQLLKRG"
     gene            complement(4778..>5027)
                     /locus_tag="PKZ74_11920"
     CDS             complement(4778..>5027)
                     /locus_tag="PKZ74_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93505.1"
                     /translation="LIGLLLPVFLYGILWLIGSQVNTGSWWALPFETDRMMMLSLAVN
                     IIPIRIYFVSLKYDRTGRGILLSTFLLMVGGFLFIRFF"
BASE COUNT         1313 a         1183 c         1154 g         1377 t
ORIGIN      
        1 ggtgttcctg gcattggaat aaatatttgt gtaatcatcc ccgttgtgat actcaatgat
       61 ggctacttcg tgcccgtttg cgatcagatc atcagcaccc atggcagccc ctgggcaata
      121 cggacaccat gttccggtac cgatttcgac aatcacttta tcgcggggta ctgtctgacc
      181 gtaaataagg ccggagagta agagcgctag agtaaataaa aagaatcttc ctttcatatg
      241 ttttcgttat ttgataatgg taattttttg tgtctgatgg gatggcccga tgttggttct
      301 gacaaagtat aatcccggcg ccagatcttt agtatttatt tcaaccgtct ggtaacctgc
      361 cattagctgg ccggcataca cagaagtaac cagttggcct gcaatatcat aaatgaagat
      421 tcgaacctct ttttgtccgt cgagccataa agttgtggtg aacttatccc tggccggatt
      481 gggaaaaata accattggag gattgctttg catttcgtca attccgactt gcgtattcac
      541 ttcaaaatag gctgtatcga tcgcaccgaa atcggtcccg tttgaaatgt aactgttcga
      601 tccgtggtac aaggcataga atccggggat attcaaaccg ttgcctcctt cgtcaaatat
      661 ccggaagata tagcattcca tgtcttccat ctggaaggtc tcctggataa cggtattcgg
      721 ctgggaatag ggtccgccgg aaaactgcaa ttcgccagcc gaattgcgga tttcccaggt
      781 cgtctcatcc gggttttcgt ctgtccggat catgagctta accgtcagcg gagtgatctc
      841 ggccctgctg aaagactgga tgatcgtatc gtttccgatg tattcgtcag gtacgccatt
      901 cgggtcgacc gtatatgcat aaaggctgtt ctcgtcaata atattgaagc tgatttcagg
      961 aagtgtgata acatccgatc ccaggaagcc gagattaccg gtccactcat acaggtatgt
     1021 cgtttcatca tttatccggt aatatacatc cacctgggtt agcggctcgg ccccgttatt
     1081 cctgatcgtc agttcaggct tgacagtccc cagacaattg gtttcgctca cattcgaaac
     1141 cttcgttatg ctggcttcat ggtcgtagag cggagtgaaa ggttcagccg tgctgttggc
     1201 tgcctggagg acatctttat tcacattatt ctgaataaat cctacaactc ccagttcttc
     1261 aatcttataa acattggcca gttcccatga cccctgcagg ataagatatt cacctggctg
     1321 aaaggaggca gggagggtgg tgccgaattg atccgggatc atttttttca ttacatcatg
     1381 aaaattcttt tcgccgtttg atccgggagg cgttgtaaaa ttgatgtgtt tttccacaac
     1441 gaccatgtgg gcgatgaggg tgccgctaac ggcctgtgtt gcttcaatca tcatatctac
     1501 gtagatgctg tcctcatcat ccgaaatcct gtggctgagc cagagattga acggtgaagg
     1561 gatggcagca tattcgtcaa gcagttcctg ggtatatccg tgcggacctc ccggataact
     1621 gaaagtcggg ccgtttggaa tatcgccgtc gatggtggcc gttggaaccc catttatgcc
     1681 gtaataagag acccgtgcat tgttttcaac tgtattgtgg ttatgcattg ggtcatatcc
     1741 gggaaaatac cagtggtact tgatagcggt gagaatatcc ctgttttggt tgaggaggtt
     1801 gtcgaaagca gggttttgcg aggcacacgg tccgcaggat gcattggtag cctcttcgat
     1861 cagcaccatt ctctgggatt gggaatgaac cgaaaatacc agaaaagaaa atgcaaataa
     1921 tgctaggtag attgttttca ttggtatgat ttttgaaaaa taaaggacgt aaaattaaaa
     1981 atattttagt cgtttgttat atatttgcat aattaaataa gacttgtaat aaagattgtc
     2041 atgaagaaaa ttttactctc tctctttgct gtttgcttct tgttaagcgc taatactcaa
     2101 agctttgtcc tgtcctatga ggggaatacc ctggcgccgg atgccgtcct cgtagttgct
     2161 gatgacccta ctgtggaagt aattgaagcc tgggtgaata tcaccaataa tgcggccacg
     2221 gccaaatcgg tactggtgaa gaaagtgatt catgatggcg ataccctggc cggatccgac
     2281 aatacattct gctgggggtt gtgctatccc aacacgactt atgtaagccc gttcccgcaa
     2341 accatccagc cgggagccac aagcgacctg ttttatggcg attacaggcc gaataacctg
     2401 atcggtatat caaaaatcac ctatgttttc tgggacgaga tgaatgtgaa cgattccgta
     2461 gccgtgaatg tgcattataa agccagcccg gcatcagtga atgacctggc cgcaaaaatc
     2521 atggcttccg gtgtttaccc caatcccgcc tccggtatgg cttacatcga ttactccatt
     2581 ccaaattcgg gcggtaacaa ccggctggtc atcaccaata tgctgggcgc tagaattaaa
     2641 gagatcggct taaaaaacat atccggcaaa gcaacgctgg atgtttctga actgatgaac
     2701 gggatctatt tttattccat tatctccgat aaccagcagg tgatcaccgg gaagttcctc
     2761 gtaagacatt gatataaaat tccaaacaaa aaagccttac aatttttgta aggctttcct
     2821 gagatcaagg ttatgtgctt tttatattaa gatttcttaa cattaactgc atttaatcct
     2881 tttctgcctt cctgaagatc aaaggttact ctgtcgtttt ccttgatttc atcttttaat
     2941 cctgatacat gaacaaaata ttccttgtcc gatgcatcgt ctttaataaa tccgaaacct
     3001 ttggagttat taaagaattt tactgttcct gtgttcataa tgatgtattg attattaaat
     3061 ttttgcaaag atatgttttt ttgaattgat aacgtgataa tcaatctttt ttaatttcct
     3121 gccggccggt ccgtttgttg ttcaacaggc ggcagaagaa ccagccggtg aaaagcccta
     3181 tgaaaacacc gccggccaca tcgcttggat aatgaacccc cagggccatc cgcgaatacg
     3241 ccacaagaaa tgcccaggta aaaacagcta cagctatgct ctttctgcgg gtgaccagcg
     3301 ttaaagccat cgcgatggcg aatgcttcca gcgtgtgccc cgatggaaat gaagcacttc
     3361 ctcccgacga caatttctca atgtcaggat aggtaatgaa cggtctttcg cgggctatca
     3421 gggtttttaa tacgaggctt atcaatgcag ccgcaaccag cgcagccatc attttaagga
     3481 agtccctgcg gtgcttccag gtcttttttt tcacagcaat gaccaggaac acgatcacaa
     3541 tcccgatact tacaaaagag gccgaatatg aaaggatata cagggcttcg tcaaggccgg
     3601 caatcctgcc gtggtgaatg aaccggagaa gttctatgtc gaaggttgaa agcattgttt
     3661 atccccgctt taaaagttga tggatgatgg ccgcggctct tgccgctgcc cctggccctc
     3721 ccagccgttc ccggagggaa tgatacccgc tgatcatttc agtgcgacgt gggccctgga
     3781 tgattttttg catctcggcc tccagtttgt cagccgttag atcattctgg attagctccg
     3841 tcacaacttc tcggtccatg atcaggttca ccagcgaaat gtatttcaca tccaccaggc
     3901 ggcgggctat cgcataagaa atggcgctac cccggtaaca aacaacctgg ggtgtccctg
     3961 tcaaagctgc ctcaagcgtg gcggtgccag atgccaccag ggctgcatgc gaatgacgga
     4021 gcagatcatg agtctgtccg aagagtatcc ggacatcctt atctcctgtc accgaccggt
     4081 aatattcccg atcaagggaa ggagcaccgg caacaacaca ctggtaaccg ggatatcttt
     4141 cagccgcccg gatcatcacc ggcaatatcc tttttatttc ctgcttcctt gatcccggca
     4201 acagtgcaat cacaggcata tcatgcaatt catttttttt caggaaggtt tccctggaac
     4261 ccgaatcctt tgcttcattc agaatatcca tcaagggatg cccgacaaaa tccacatcaa
     4321 attgatattt tgaatagaaa gctttctcaa aaggaagaat gaccagcatt ttatccacat
     4381 atttcctgat actatagacc ctcgattttt tccaggccca aacctgcggt gaaatatagt
     4441 acacaacttt aaatcctttc cggctggcgt attttgccat gcgcaggttg aaacccggat
     4501 aatcgaccag tatcagaaga tccggtctga aatcgttgat atcttttttg caatagttca
     4561 ggtttttcag aatggtcttc aggttgacag ccacctccag aaaccccata aaagccaggt
     4621 cgcgatagtg tttgaccagg tggccgcctg cctgctccat catatcgccg ccccataccc
     4681 tgaaaactgc gccaggatca ttgttcctta tttcgctgat caggcgggag ccatgaaggt
     4741 ctcccgaagc ttcgccggca atgatatagt atttcatcta aaagaaccgg ataaagagaa
     4801 aaccgccgac cattaaaaga aatgtggaaa ggaggatgcc ccggcctgta cggtcatatt
     4861 tcaggctgac aaaatagatc cggatcggga tgatattgac agccagcgac agcatcatca
     4921 tccggtcggt ttcaaatggc aaagcccacc aggaaccggt attgacctgc gaaccaatca
     4981 gccacaatat cccgtacaga aagacaggga gcaacaggcc gatcaga
//
LOCUS       DAOPNH010000254         5010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19491_length_5010_cov_5.509990, whole genome shotgun sequence.
ACCESSION   DAOPNH010000254 DAOPNH010000000
VERSION     DAOPNH010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_5010_cov_5.509990"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..2273
                     /gene="ppk1"
                     /locus_tag="PKZ74_11925"
     CDS             165..2273
                     /gene="ppk1"
                     /locus_tag="PKZ74_11925"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPM93506.1"
                     /translation="MELNDQYFFNREISWLHFNERVLQEAIDPATPLLEQVKFLGIYS
                     NNRDEFFRVRVATLKRMIKLEQNETVPDLKYKVILDEVLEIVGGQEKLFIKAYNKVVR
                     ELARNDIYIINEKQVTPEQGEFITQFYKEKVRPNLFPLMLGNVRSSLSLQDASIYLTV
                     ILKKSAEPTVEDYALIQVPSDINGRFLVLPMDGRKSFIILLDDVIRYCLKDIFSAFGY
                     DRFESYTIKFTRDAELEIDNDVSKSFLELISVSVKKRKLGEAVRFVYDNTMPDDLLKI
                     LIKKLNIAKKDTIRGGGRYHNFKDFMDFPRIGDEKLRYPPAPPVPHRDLVNQPSMFKA
                     IRRKDIMLFFPYQSFQYIIDLLREASIDPDVRSIKMTFYRTTRNSAAMNALINAARNG
                     KYVTVFMEIQARFDEEANIYWTRRLQDEGIKVIQTIPGYKVHAKLILIRRRENGQNRL
                     YANVSTGNYNETTAKVFSDFSLLTCDPRICDDVYNIFELLESKFILPTFKNLIVSPFN
                     IRGFFIDMLDREIQNAKKGLEAWAIIKLNSLVDRRAAAKLYEAARAGVQINLINRGIC
                     VIKPGMPGVSLNINAFSIVDKYLEHSRVYIFCNGGYPEYFISSADWMPRNFDHRIEVV
                     CPIYDTGIQQELMDVLKIQMQDNTKSRYLGPEQLNRYRTTDDPVKHRAQFEIYDYYHK
                     KVKIKPHGQPGKSKSNCKEG"
     gene            2230..2697
                     /locus_tag="PKZ74_11930"
     CDS             2230..2697
                     /locus_tag="PKZ74_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93507.1"
                     /translation="MVNPENQNQTARRDKKIHIPSPARKIGYIVVIIILIVILYLIRN
                     YEKWNLTFLTKEFSKCLFYIELSIYINIGANILFIFYDNKWFKHLLQAITDVAGALSL
                     IMIYVIYPLNISNGDYDKWIRTGLLIIFVLTVISILINLIKGIRYLVREPEAV"
     gene            2722..>5010
                     /locus_tag="PKZ74_11935"
     CDS             2722..>5010
                     /locus_tag="PKZ74_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93508.1"
                     /translation="MQLPVSRSPRLQISWHSFLLLFVFCALRFELTQAQYFSLGTDAA
                     SVKWDRIKTENFSVIFPRQLDSQALRTANTLEYFREMALEGRNIRTGRWPVILHNGTV
                     IPNATTPFAPKRIDMLTIPPQDQYGQDWLDQLVIHEFRHSVQYATLDKGFTRAMRILF
                     GQQAVPAVLGLFVPFWYIEGDATVTETAMTRTGRGRSPSFEMRLRAQVIEKGIYSYDK
                     AYNGSYRDFIAGHYEMGYLLVGSGMLKYGGSNLIKPLQKSGTLPFTFVPFSHALYKAT
                     GTGKRKFYRNTLTELGEKWKEEDRNLDTTACSRIREGISRTYTNYTSPIPLPDGLVAA
                     LRSSLDDITRIVLIDKDGRETIACTPGPMIDPVISARGNMICWAELENDPRWALRDYS
                     VIKVHDQQSGRTRQITHKSRYFSPDPDPSAKAIAVVEVDMQNSYYITLVNAADGHAIK
                     RFPVPGNHYPSHPVWSNDGRRIAVILTRSEGKCIAVLDIETAKFEIALPFSYTEISKP
                     TWHEHYILFTGAYTGIDNIFALDLNTGELYQATSARFGATDAAISADGKTLYYASYSS
                     GGYDIVATGFSALSDASHPGDPDSLNPWNPQNNYSFELADGLSEQVGTRFDASAVPDS
                     GYTIRRYCKGLNLFNFHSWAPLYLDIDHLGIQPGVTLMSQNLLASSFATIGWAYDLNT
                     ESGKYMLKYSYEGLYPAFDINLDYGLRHGVIDHTIQENIIYKYHELNTGGWVRVPLNW
                     NIGSWFAGVQPFAGYSYIFRRML"
BASE COUNT         1342 a         1265 c         1190 g         1213 t
ORIGIN      
        1 cccctttgtt gcaagcataa caatatttgt ttgaataaaa aaacaaacat ataagttcca
       61 cgaaggactt ttaagccacc ctcttcatat ttcaactgac tatcaaagta aattcataaa
      121 tttgtgagat aaattcaaaa tgaaggaccg gataaaatca gcttatggaa ttgaacgatc
      181 agtatttttt taaccgtgag ataagctggc ttcatttcaa tgagcgggtt ctgcaggagg
      241 cgatcgatcc cgccactccc ctgcttgaac aggtgaaatt tctcgggatt tattccaaca
      301 accgcgatga gtttttcagg gtcagggtgg ctaccctgaa aaggatgatc aaactggagc
      361 aaaacgagac tgtacccgat ttaaagtaca aagttatcct ggatgaagtg ctggagatcg
      421 ttggcgggca ggagaagctg ttcattaaag cctataacaa ggtagtaagg gagctggcca
      481 ggaacgacat ttatattatc aatgaaaaac aggtcactcc ggaacagggt gaatttatca
      541 cgcagtttta taaagaaaag gtacgtccca accttttccc gctgatgctc ggcaatgtga
      601 ggagttcgct gtcattgcag gatgcttcga tctatctgac ggtcatttta aagaaatctg
      661 ccgagcccac ggttgaagat tatgcactga tacaggttcc cagcgatatc aacggccggt
      721 tcctggtcct gccgatggac ggccggaagt cgttcatcat cctgctggat gatgttatcc
      781 gttattgcct taaagacata ttctccgcct ttggatatga caggtttgaa tcctacacca
      841 tcaaatttac acgcgatgcg gaactcgaga tcgataacga cgtttcgaaa agtttcctgg
      901 aactgatcag tgtcagcgtg aaaaaaagga aactgggaga ggctgtgcgg ttcgtttacg
      961 ataataccat gccggatgat ctgctgaaaa tcctgatcaa gaagcttaac attgccaaga
     1021 aagacacgat ccgcggaggt ggaaggtacc acaatttcaa ggatttcatg gactttccga
     1081 ggattggcga cgaaaagctg aggtatccgc cggcgccacc ggtcccgcac cgcgacctgg
     1141 ttaaccagcc cagcatgttc aaggccatcc ggcggaaaga catcatgctg ttttttcctt
     1201 accagtcgtt ccagtatatc atcgatttgc tgcgggaagc atcgatcgat ccagatgtcc
     1261 gttcgatcaa aatgaccttt taccggacca cgcgcaactc tgcagccatg aatgccctga
     1321 tcaacgcggc acgcaacggg aaatacgtaa cggtttttat ggagatccag gcccggtttg
     1381 atgaggaggc aaatatctac tggacccgca ggctccagga cgaaggcatt aaggtaatcc
     1441 agaccattcc cggctacaag gtgcacgcca agctgatttt gatcaggcgc cgcgaaaacg
     1501 ggcaaaaccg gctctacgcc aacgtcagca caggcaatta caatgaaaca acagcaaaag
     1561 tattttccga tttcagcctc ctcacctgcg acccgagaat ttgcgatgat gtatacaata
     1621 ttttcgagct gcttgaaagc aaattcattc tgccgacatt caaaaacctg atagtatcgc
     1681 cattcaatat acgcgggttt ttcatcgata tgctcgaccg ggagatacag aatgcgaaga
     1741 agggtttgga ggcatgggcc ataatcaaac tgaacagcct ggtcgaccgc agggcagcag
     1801 ccaagctata cgaagccgcc cgggcaggcg tacagatcaa tttgattaac cggggcattt
     1861 gtgtcattaa gccgggaatg ccaggtgtca gcctgaatat caatgccttt tccattgtcg
     1921 ataaatacct cgagcacagc cgggtctata ttttctgcaa cggtggctac ccggagtatt
     1981 tcatctcttc ggccgactgg atgccgcgga atttcgacca ccgcatcgaa gtcgtttgcc
     2041 ctatttacga caccggcata cagcaggaac tgatggatgt tctgaaaatc cagatgcagg
     2101 ataacactaa atcgcgctac ctgggaccgg agcaactgaa ccgctaccgc accacggatg
     2161 atcctgtaaa acaccgtgcc cagtttgaaa tctacgacta ttatcataag aaagttaaaa
     2221 tcaagcccca tggtcaaccc ggaaaatcaa aatcaaactg caaggaggga taaaaagatc
     2281 catatccctt cgccggcccg gaaaatcggg tatatcgtcg tgatcatcat cctgatcgtc
     2341 atcctttatc ttatccgcaa ttatgaaaaa tggaacctga cgttcctcac gaaagaattc
     2401 agcaaatgcc tgttttatat tgaattatcc atatacatta acataggggc caatatcctt
     2461 tttatctttt acgataacaa atggttcaag cacctgctgc aggcgattac cgatgtagcc
     2521 ggcgccctct ccctgatcat gatctatgtc atctaccctt tgaacatcag caacggcgac
     2581 tacgataaat ggatcaggac gggattgctg atcatctttg tccttaccgt tatttccata
     2641 ctgatcaatc tgatcaaggg gatcaggtac ctggtgcggg agccggaagc cgtctgacaa
     2701 gcaataaaga attggaaagg tatgcagctc cccgtctccc ggtctccccg tcttcaaatc
     2761 tcctggcatt cctttcttct cctctttgta ttttgcgctt tgagatttga actaacccag
     2821 gcgcagtatt tttcactggg aaccgatgcc gcttcggtaa aatgggatcg gatcaaaacg
     2881 gaaaacttca gcgtcatctt tccccggcag ctcgattcac aggctctgcg caccgccaat
     2941 accctggaat acttccgcga aatggcactg gaaggtcgaa atatcaggac tggccgctgg
     3001 ccggtcatcc tgcataacgg aactgtcatt cccaatgcta ccaccccttt tgccccgaaa
     3061 aggatcgata tgctgaccat tcccccccag gaccaatacg ggcaggactg gctcgaccag
     3121 ctggtcatcc atgaattcag gcacagcgtg caatacgcca cgctcgataa aggatttacc
     3181 cgcgccatgc gtatcctgtt cggccagcag gccgtccccg ctgtcctggg cttatttgtc
     3241 cccttttggt atatcgaagg agatgctacg gtgaccgaaa cggccatgac ccgtaccgga
     3301 agaggccggt cgccttcctt cgaaatgagg ctcagggcac aggtcattga aaaaggcatc
     3361 tattcgtatg acaaagctta taatggttca taccgtgatt tcatcgccgg tcattacgag
     3421 atgggatacc tgctggttgg aagcggcatg ctgaaatatg gtggctcaaa cctgataaaa
     3481 cctttgcaaa aatcgggcac gctgcctttt acgtttgtcc cattttcaca tgcgttatac
     3541 aaagcaaccg gtacaggaaa aagaaagttt tacagaaata ccctgaccga actcggggaa
     3601 aaatggaaag aagaagaccg aaaccttgat accacggcct gttcgcgcat tagggaagga
     3661 atctccagga cgtacacgaa ttacaccagc cccatcccac tgcccgacgg cctggttgcg
     3721 gctctcagat cttcgctgga tgatattacc cgcattgtgc tgatcgataa ggacggtcgg
     3781 gaaactattg cctgcactcc cggtccgatg atcgatccgg ttatatcagc ccgggggaac
     3841 atgatctgct gggccgaact ggaaaacgac cctcgctggg cgctgcgcga ttattcggta
     3901 atcaaggtcc atgaccagca atcgggcagg acccggcaga taacccacaa aagccgctat
     3961 ttttcaccag atcctgaccc atctgcgaaa gcaattgccg tggtggaagt tgatatgcaa
     4021 aacagttact acattaccct ggtgaatgcg gctgatggcc atgccatcaa acgctttccc
     4081 gttccaggca atcattatcc gtcgcacccg gtgtggagta acgacggccg gcgaatagcc
     4141 gtgatcctga cccgcagcga aggaaaatgc atagcagtac tggatattga aaccgcaaaa
     4201 tttgagattg cccttccgtt ttcatatacg gaaatatcaa aaccaacctg gcatgaacat
     4261 tatatccttt tcaccggagc ctataccggc atcgacaata tttttgcact cgatctgaat
     4321 acaggggaat tgtaccaggc cacctcggcg cgtttcggcg ccacagatgc ggccatttcg
     4381 gcagatggaa agacgctgta ttacgccagc tattcatccg gcggttacga tattgtcgca
     4441 accggattct cagcgctatc tgacgcgagc catccgggtg atccggattc tttaaacccc
     4501 tggaacccgc agaacaatta ctccttcgaa ctggccgacg ggctgtcgga acaggtggga
     4561 acccggtttg atgcttctgc agtaccggat tcgggctata ccatccggcg ctattgtaaa
     4621 gggctgaacc tgttcaactt tcatagctgg gctcccctct acctggatat tgaccacctg
     4681 gggatccagc cgggcgtcac cctgatgtcg cagaaccttc tggcttcgag ctttgccacg
     4741 atcggctggg cttatgactt aaatacggaa tcgggcaaat acatgctgaa gtatagttat
     4801 gaaggtcttt acccggcatt tgacatcaat ctggattatg gcttacggca tggcgttatt
     4861 gatcatacca ttcaagaaaa tattatttac aaataccatg agctgaatac aggcggatgg
     4921 gttcgggtgc cgctcaactg gaacatcgga agctggtttg ccggggttca gccatttgcg
     4981 ggttattcct atatattccg tcggatgttg
//
LOCUS       DAOPNH010000255         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19780_length_4951_cov_4.650531, whole genome shotgun sequence.
ACCESSION   DAOPNH010000255 DAOPNH010000000
VERSION     DAOPNH010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19780_length_4951_cov_4.650531"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKZ74_11940"
     CDS             complement(<1..574)
                     /locus_tag="PKZ74_11940"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM93509.1"
                     /translation="MKTFFLTLLAGLMISMTAIAQNYPLTVTGTVVQVTQNTMIPLAD
                     YPVTIRIDSTGFGFSYENTVYTDDNGYYEDTAEIPGYNGYGYVTAETYDSCLGYTVSQ
                     GQFIAPGTTLMPMDFFLCTVINPECQAYFYYYQSNPVDPYTFTFENMSMGEYTEFFWD
                     FGDSTYSYEMYPQHTFPGPGYYNVCLTIGGT"
     gene            complement(649..3912)
                     /locus_tag="PKZ74_11945"
     CDS             complement(649..3912)
                     /locus_tag="PKZ74_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM93510.1"
                     /translation="MKKLIILILTGILVTGFSQGQGGWREYEMEVKIVFGRLEDASKL
                     GALNLNGDIYSAKGYAILYVTPDELDLINSKGFKTEILKQDLNLYYHDFWTSRDQYHT
                     YDEIIDVIDSLVETFPQIAKKYTYGTSLGGRQLCALKISDNVYADEPEPEVIFDAGIH
                     GDEIGGPENLVRFAIFLCENHTVDPYITGLINTREIWLYIMVNPDGRVNMTRYNDAGV
                     DLNRDYGYMWNGAGSSPAAYSQPETRAIRNCLLDHQFVLSSSYHSGTEFLAYTWSYRP
                     DTCPDRPQIEYLADLYSTTSGYDLLEYGQGYTGMYPINGSSKDAAYGSLGSVGWTMEI
                     SYAKQPPASQIQYFYDINEPAMLAVIDQAGNGLHGTVTDAETGYPVSAAIFVDNGYPC
                     YSDPVIGDYHKFLLPGTYTVKITANGYEPQTTEVLIDNTGNAVLNFSLQPADEVHAWR
                     VIACQIPASNFSDEAFTAAAIGPPDNANYSLGHLGWIILDMGRIVFDGPGDEIIIYEG
                     DNGPEGFTCHVSDNMDGPWQLAGSGTGTSAFDFFAGGIAGARYIRITDDGAGQVSGDN
                     AGFDLDAIEAPAQPELIFVTMDARIDDQAANGNARIDPGETATLVITLQSLGSVEVTG
                     GLAYLNYNAGYISIPNPETGFENIGYGESTELNFTMSCSSFCPAGSLVMMALNITTNN
                     GEYMQTFPFSFTVGPVDEDWESAGFSQFSWMPSGNKPWVINFQTPYEGNCSARSGNID
                     DGEVSALEITLDVIGYDDISFYRKVSSQGNSDYLRFYIDNNLVDYWSGELSWERMEYD
                     VLPGFHTFKWTFEKDGSGSSGSDAGFVDFIVFPSCNLDATFKVLANAWPNSYCGSGES
                     QLSAFTIGGAGDPVFSWSPPEGLSDPNDQYPVATLTQSAILTVTASDGGQPASSITAI
                     NVFNLPATPVILQQGDSLISQADSGNQWYTLEGPVTGATEKVFYPETEDDYFVIITNE
                     NGCESDSSNIIHFIFTGIQEEAEDELFKVFPNPFCERIIIQNKSNTKSELIVTLKDIL
                     GREIYRGTMKETDRQKIIDTSGLNLENGLYLIAVLDKECRLLSSSKLIKSYNILLSK"
     gene            complement(4011..>4951)
                     /locus_tag="PKZ74_11950"
     CDS             complement(4011..>4951)
                     /locus_tag="PKZ74_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93511.1"
                     /translation="FDVATLNYYSLGGLVYAGDYPLNNPDNQGDVGIASLYRLVNDQI
                     VFVEDQTFQEYGYFWFGYLFPGDYMVKVGLTPESPSYNEYFTTYYGDEVSWTKADILS
                     VSSVSQFDAEVHLAPVQELSSGAGTIKGYVKFEQGNQYNMPPIAQTNVILADKNHVPL
                     LFTHPNAAGYFEFSSLPVDSYYLSADATGKPSSTVLVTLTQAVPVVEGINLTVFGSNI
                     SFIPDETSGNQLMIKLFPNPAGDNLYISMYSVYSDPIEVTICDITGKIQHRSSVVFEP
                     GTNTWVIPVNNLPAGIYLLRIQQPGILQPITARFIK"
BASE COUNT         1266 a         1210 c         1199 g         1276 t
ORIGIN      
        1 tagtcccgcc aatcgtcagg catacattat aatatccagg gcccggaaaa gtatgttgcg
       61 gatacatttc ataagaatag gtactgtcgc cgaaatccca gaagaattcc gtgtattctc
      121 ccatcgacat gttttcgaat gtgaatgtgt aaggatcgac ggggttactc tggtaataat
      181 aaaaataagc ctggcattcc gggttaatta ccgtgcagag gaaaaaatcc atcggcatca
      241 atgttgtccc cggggcaatg aattgcccct gcgaaacggt atatccgagg catgagtcat
      301 aggtttcagc cgtaacataa ccatatccgt tgtaacccgg tatttctgca gtatcttcat
      361 agtaaccgtt gtcatcggta tagaccgtgt tttcatatga aaatccaaaa ccggtgctat
      421 cgatcctgat cgtgacggga taatcggcaa ggggtatcat cgtgttctgg gtgacctgca
      481 cgacagtccc tgttaccgtc agcgggtagt tctgggcgat cgcggtcatg ctgatcatca
      541 gcccggcaag aagtgtaagg aagaatgttt tcatgacaat agatttgaag tttttgatct
      601 tttataatcc ttaaacacct tttcaccgaa aggttgcctt ttatttgatt attttgaaag
      661 aagaatgttg tacgacttta ttaatttgct ggatgatagt aatctgcatt ccttgtccag
      721 cacggcgatc aaatataatc cattttcgag attcagtcct gaagtatcga taatcttctg
      781 ccgatcggtt tctttcatag tcccccgata aatttcccgt cccagtatat ccttcagggt
      841 tacaattaat tccgattttg tattggattt attttgaatg atgatccttt cgcaaaacgg
      901 attggggaat actttaaaga gctcatcttc cgcctcttcc tgaatcccgg tgaagataaa
      961 atgaattatg ttggatgaat cggattcgca accattttca ttagtaatga tgacgaaata
     1021 atcgtcttca gtttccgggt aaaagacttt ttctgtcgcc ccggttacag ggccttccag
     1081 ggtataccac tggttgccgc tgtcggcctg gctgatgagc gaatcgccct gttgcaggat
     1141 gaccggagtc gccgggaggt tgaatacgtt gatggcggta atagaagaag ccggctggcc
     1201 tccatcgctg gcagtcacgg tcaggatggc gctttgcgtt aacgtagcta ccgggtattg
     1261 gtcattcgga tcgctcagcc cttccggcgg cgaccaggag aagaccggat caccggcgcc
     1321 acctatggtg aaagcgctca gctgcgattc tccgctgccg caatagctat taggccatgc
     1381 attggccagt actttaaaag tggcatccag gttgcaggag gggaaaacga taaaatcgac
     1441 aaaccctgca tccgaacccg aactgcccga accatctttc tcaaatgtcc atttaaaagt
     1501 gtgaaatccc ggcaaaacat cgtattccat tctttcccag cttaattcac ccgaccagta
     1561 atctaccagg ttgttgtcga tgtaaaatct gaggtaatcg gaattaccct gggaagaaac
     1621 tttccggtaa aatgagatat catcgtagcc gatcacatcc agcgtaatct ccagcgccga
     1681 aacctctccg tcatcgatgt ttcctgaacg ggcagaacag ttgccttcat acggcgtctg
     1741 gaaattgatc acccacggct tgttgcctga aggcatccag ctgaattgac tgaatccggc
     1801 cgattcccag tcttcatcca ccgggccgac cgtgaagctg aaaggaaagg tttgcatgta
     1861 ctccccgttg ttggtagtga tattcaatgc catcatgacg agggaccctg ccggacagaa
     1921 agagctgcag ctcatcgtaa aattcagctc cgtgctttca ccgtatccaa tattctcaaa
     1981 accggtttcc gggttgggga tgctgatgta gcccgcattg taattgaggt acgccagccc
     2041 tcctgtcacc tcaacactgc cgaggctttg caaagtgatc accagcgttg ccgtttcgcc
     2101 gggatcgatc cttgcgttgc catttgcagc ttgatcgtcg atgcgtgcat ccatagtaac
     2161 gaaaattaac tccggttgtg ccggcgcttc gatggcatcc aggtcaaaac cggcgttgtc
     2221 gcccgaaacc tgccccgctc cgtcatcggt gatccggata taccgtgctc ctgcaatacc
     2281 gccggcaaag aaatcaaagg cagaggtgcc ggtgccactc ccggcgagtt gccaggggcc
     2341 atccatgtta tctgaaacat gacaggtaaa tccttccggt ccgttatccc cttcgtatat
     2401 gatgatttca tctcccggtc cgtcgaatac aatcctcccc atgtccagga tgatccatcc
     2461 caggtggcca agcgaataat tggcattgtc gggcggaccg atggccgccg ccgtgaaagc
     2521 ttcatcggaa aagttagaag ccggaatctg gcaggcaatg actctccagg catgaacctc
     2581 atccgctggc tgaagcgaaa aattgaggac agcgttcccg gtattgtcaa tcagaacctc
     2641 agtagtttgt ggttcatatc cgttggccgt tatcttcacc gtgtacgtcc cgggtaacag
     2701 gaatttatgg taatccccga tcaccgggtc actgtaacaa ggatagccat tgtcgacaaa
     2761 aatggcggca gacacagggt agcctgtttc cgcatccgtc accgtgccgt gcaggccatt
     2821 gccggcctgg tcgataaccg ccagcatagc cggttcgtta atatcgtaaa aatattgtat
     2881 ctgcgaggca ggaggctgtt tggcatatga gatttccatg gtccagccaa ccgaacccag
     2941 gcttccgtaa gccgcatctt ttgaacttcc attgatgggg tacattccgg tgtaaccctg
     3001 tccgtattca agcagatcat aaccggaagt ggtgctgtag agatcggcaa ggtattcaat
     3061 ttgtggcctg tcaggacaag tgtcgggcct gtaactccat gtataggcaa gaaattcggt
     3121 gccgctgtgg tagctggaac tcaacacgaa ctggtgatcc agaaggcaat tgcggatagc
     3181 acgcgtttcc ggttgtgaat aggctgcagg gcttgatccg gccccgttcc acatgtaccc
     3241 gtaatcacgg ttcaggtcaa caccggcatc attgtaacgg gtcatattta ccctgccatc
     3301 gggatttacc atgatatata accagatctc acgggtattg atcagaccgg ttatataggg
     3361 gtcaacagta tgattttcac agagaaagat ggcaaaacgg accaggttct cagggccacc
     3421 gatctcatcg ccatggatcc ccgcatcaaa aatgacctcc ggctccggct catcggcata
     3481 aacattgtct gaaattttca gggcacacaa ctgcctgccg cctaaagaag tgccgtaagt
     3541 gtatttttta gcgatctgcg gaaaagtttc caccagggaa tcaatgacat caatgatctc
     3601 atcatatgtg tgatactggt cccgggaagt ccagaagtca tggtaatata ggttcaggtc
     3661 ctgtttcagg atttccgttt tgaaaccttt gctattaata aggtccagtt catcaggcgt
     3721 tacatacaga atagcgtagc ctttagcaga gtatatatcg ccgttaagat tcagtgcacc
     3781 caatttactg gcatcttcca accggccgaa aacgatcttt acctccattt catattcgcg
     3841 ccagcccccc tgcccttgcg aaaatccggt aaccaatata ccggtaagta taagtatgat
     3901 gagttttttc atttgaaaat aatgcttcgc cggtcagcag ttcataaaga tgaacctgcc
     3961 ggggttgatc cacgctcaaa agtacgaaag aaattccccc ggggggccta ttattttata
     4021 aagcgtgccg taataggctg taatataccg ggctgctgaa tgcggagcag gtaaattccg
     4081 gccgggaggt tgttcacagg aatgacccag gtgtttgttc ccggttcaaa aaccactgat
     4141 gaccggtgct ggatctttcc cgtgatatcg caaatggtaa cctctattgg atcagagtaa
     4201 accgaataca tgctgatgta aaggttatca ccggctgggt tgggaaatag ctttatcata
     4261 agctggttac cacttgtttc atcggggata aaactgatat ttgaaccgaa caccgtcagg
     4321 ttgatccctt ccaccaccgg aaccgcttga gtaagggtca caagaacggt cgacgaaggt
     4381 tttccggtgg cgtcggcact cagataatag ctgtcgacag gcaatgagga aaattcgaag
     4441 taaccggccg cgttgggatg ggtaaacaac aacggaacgt ggtttttatc agccaggata
     4501 acattggtct gggcaatggg cggcatattg tactggttgc cctgttcgaa tttaacgtat
     4561 cctttgatag tgcctgcacc ggatgaaagt tcctgtacag gagcaaggtg tacctctgca
     4621 tcaaactggc tgacggatga aacggagagg atatctgctt tcgtccagga tacttcatcc
     4681 ccgtaataag tggtgaaata ttcgttataa ctcggcgatt caggcgtcaa tcccactttc
     4741 accatataat cgccggggaa tagataaccg aaccagaaat atccgtattc ctggaaagtc
     4801 tggtcttcca caaaaacgat ctggtcgttg accagccggt acaacgaagc gataccgaca
     4861 tcaccctggt tatccgggtt attcagcgga taatcaccgg catacaccag gcctcccagg
     4921 ctgtaatagt tgagcgtggc aacatcaaaa c
//
LOCUS       DAOPNH010000256         4943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19817_length_4943_cov_6.392390, whole genome shotgun sequence.
ACCESSION   DAOPNH010000256 DAOPNH010000000
VERSION     DAOPNH010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19817_length_4943_cov_6.392390"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKZ74_11955"
     CDS             complement(<1..1177)
                     /locus_tag="PKZ74_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM93512.1"
                     /translation="MIVAILDWGFDWEHDDLGPGTDGYENIYLNDQDTWTIWDEPNSG
                     DESDNDENGYIDDWKGWGEMHDVNHYPVFYQGNDVRNYDYDWLLEYCQYDEPLAKAFW
                     MHGTFLSGIIGAKTNNGYGISGIAGGWHDKGVSMLFYKLGVTGEEGALTSDVAWGIEY
                     AMYKLPHEHKAKIIELALQTLENSFITNALQDAYNNGAFIVAAAGNHGEGSDFSVSYP
                     ANLDFVFAVGATTDQDLRKSNSNYGDELDIGAPGENIFGLVPQTNSYNGFELIEGGNT
                     SAASAMVAGTAALMCSVNPDLTNQAIADTLKAAADKGTYYTYDENGHNDEIGHGRLNA
                     YKAVCKAGQSAPILEIEADETWDYSRSFLRSVHVKSGVTLTINADIQFGPEAALVIES
                     "
     gene            complement(1229..1651)
                     /locus_tag="PKZ74_11960"
     CDS             complement(1229..1651)
                     /locus_tag="PKZ74_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93513.1"
                     /translation="MKKIINPILLLSFSFICSLPIQASINIVGNDTIHYDNEKYFIIY
                     QTDTFYVDTSLIFIRYNGIANTINIESIENEYDLNLNHYFTFGWHSYTYDPGYRFVEL
                     ATDLSGENLVEAVEYNTSVYPACEPLEAADNYSLDQWY"
     gene            complement(2151..2714)
                     /locus_tag="PKZ74_11965"
     CDS             complement(2151..2714)
                     /locus_tag="PKZ74_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPM93514.1"
                     /translation="MILIGDTAVSDDLHLVRFCCHLARCQGACCVAGDAGAPLEEEEI
                     AILEDELEAIKPFMTERGIATVENSGVFDYDIAGKFVTPLVNDGECAFTNFNNGIAYC
                     AIEKACSEGKTRFLKPVSCHLYPVRITKYATFEAVNYHKWNICKPALKLGNKEGIPLY
                     KFLRTALVRKYGESWYRELEKEISSRE"
     gene            complement(2711..3328)
                     /gene="udk"
                     /locus_tag="PKZ74_11970"
     CDS             complement(2711..3328)
                     /gene="udk"
                     /locus_tag="PKZ74_11970"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPM93515.1"
                     /translation="MLVIGIAGGSGSGKTTVVNKIIEGLPPKSVSIMSQDAYYWDNGH
                     LSPEEKKLINFDHPDSIEWELLIHHLDKLIKGHSIEMPRYSYVECARLKETVKIEPKR
                     VIIVEGILILTHPELRNRFNVKVFVDADADDRLTRIIQRDILERGRDVAMVLRHYETY
                     VKPMHLQFIEPSKRYADLIIPQGGENSVAIDLLSSRINWKFNQER"
     gene            3532..4335
                     /locus_tag="PKZ74_11975"
     CDS             3532..4335
                     /locus_tag="PKZ74_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93516.1"
                     /translation="MKLLLSLTLFFSIVVESIAQVPLNWTRDEINPGEDFTLFQDNSV
                     FTEGEKSLHMQLNKGSVPYLVSDIFYISPGADYTFSVDVLDNDTAGQVKIYADFYDAY
                     GFDIFGEPPVFSADSADWVTISWQGTVPSQAVVGYILIKFYNQPNLYTFTKQANIWLD
                     NVRFIQEGGPNLVANGGFEEWAVGMDDDREEMELTVFPNPADDFIGLKFTDKINRVEL
                     YNSAGQRVRLQEANGTVAGIPVAGLMEGLYLLKIETGIGVVVKKLIIHH"
     gene            4421..>4943
                     /locus_tag="PKZ74_11980"
     CDS             4421..>4943
                     /locus_tag="PKZ74_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM93517.1"
                     /translation="MNKTKFIIILISGVLILFTSCNQQPPQQEKAQNEFTFAFLTDIH
                     VQREKDAISGFRKAITKVNELKPDFVITGGDLVMDALGQTYERADSLYHIYTTMMTEF
                     NMPVFNTMGNHEIYGWYVLKDGDTNHPEYGKKMFENRIGPRFQRIDRFGWIIFILDSV
                     VKDSTGDGYEGGVD"
BASE COUNT         1329 a         1151 c         1083 g         1380 t
ORIGIN      
        1 cggattcaat aaccaatgct gcttccgggc caaattgaat atcagcattt atagtaagcg
       61 taacgcctga tttaacatga actgatctta agaaagacct ggagtaatcc caggtttcat
      121 ctgcctcaat ttccagtatt ggcgctgatt gtcctgcctt gcaaacggct ttgtaagcat
      181 ttagccggcc atggccaatt tcatcattat gcccgttttc atcgtatgtg taataagtcc
      241 ctttatcggc cgctgctttt aatgtgtcgg cgattgcttg attggtaaga tcagggttca
      301 ccgaacacat caaagcagcg gtgccggcga ccatagccga tgctgcagag gtattgcctc
      361 cttcgatcaa ttcaaagccg ttgtaagaat tggtttgtgg tacgagtcca aagatatttt
      421 cacccggagc gcctatgtca agttcatcac cataattgga atttgatttt ctaagatcct
      481 gatcagtagt tgccccaact gcaaatacaa aatcaaggtt tgccggataa gacacactaa
      541 aatcgctacc ttcaccatgg ttgccggcag ctgctactat aaaagcgcca ttattatatg
      601 catcttgtaa agcatttgta atgaaagaat tttctaaggt ttgtaatgcc aattcaataa
      661 ttttagcttt atgctcatga ggcaatttat acattgcgta ttctattccc caagcaacat
      721 cactagttaa agctccttct tctccagtaa cacccagttt gtaaaatagc atagatactc
      781 ctttatcgtg ccatccgcct gcaattccgg atatcccata accattattt gttttggctc
      841 caataattcc tgaaagaaaa gttccatgca tccagaatgc ctttgctaac ggctcatcat
      901 attgacaata ttctaataac cagtcataat cgtaatttct tacatcattc ccttgataga
      961 aaacaggata atggttgacg tcgtgcattt ctccccagcc tttccagtcg tcgatataac
     1021 cattctcatc attgtcagat tcatctccgg aatttggttc atcccatatg gtccaggtgt
     1081 cctgatcatt aaggtaaatg ttctcataac cgtccgttcc aggtccaagg tcatcatgtt
     1141 cccagtcaaa accccaatca aggattgcaa ctatcacctc gggatcgcct gtagttatat
     1201 tccaggcatc tctggcttga atttgagttt aataccactg atccaaagaa taattgtctg
     1261 cggcttctaa tggttcacaa gccggataaa ccgatgtgtt atattcgacc gcttctacca
     1321 ggttttcacc acttaaatcc gttgccagtt cgacgaatct atatcccgga tcataggtgt
     1381 aagaatgcca gccaaatgta aaataatgat tcaaatttaa atcgtattca ttctcgatag
     1441 attcaatgtt aatggtattt gcaatcccat tatacctgat aaagataagg gaagtatcga
     1501 cgtaaaaggt atcagtctgg taaattataa agtacttttc attgtcataa tgaattgtat
     1561 catttccaac tatgttgata ctggcctgga taggaagaga acatatgaaa gagaaactta
     1621 aaagtaagat tggattaatt atttttttca tggatttgct ttttaaagat ttagtaatgg
     1681 agtttaaatg ggaaacttaa accgcattac ggggggatct ttattaaact ctctctctct
     1741 ctctctctct ctctctctac agaaacgcgc tattaggtaa aaccgaatca tttggatcaa
     1801 ttggatttgg ttaaaaatga aaacgcgaaa tgcatggaac aaatataaat aagatatatt
     1861 caaaagtcaa gggatggttg ataacttttt cgtacatgag actccatctc taatttgaga
     1921 tggagtctca ttaaattcag atggagtctc acagaaatac cccattacac tatcagttcc
     1981 agatggaatt gtcttccgac aggtgaatcc gtcggttcca aagatgcaca atatggcacc
     2041 aacggattta cccgttggag caatacaaac aacaaccaaa ggatttaccc gtcgtactac
     2101 caaatcaaat cgaaaatgcc gaatgccgaa tgccgaatcg cgtaatgcct ctactccctg
     2161 gagctgatct ccttctcaag ctcccggtac cagctttccc cgtatttcct caccagggcc
     2221 gtcctcagaa atttgtaaag cggtatcccc tctttgtttc ccagcttcag agccggttta
     2281 caaatattcc acttgtggta gttcaccgct tcgaatgtgg cgtatttcgt gatcctgacc
     2341 gggtaaagat ggcatgaaac aggcttcagg aaacgggttt tgccttcgct gcaggccttt
     2401 tcaatggcac aataagctat cccgttgtta aaattggtaa acgcgcattc gccgtcgttc
     2461 accaggggcg tgacaaactt tccggcaata tcgtaatcaa aaacaccgga gttctcaacc
     2521 gttgctatgc ctcgttcggt cataaacggc tttatcgctt ccaactcgtc ttccaggatg
     2581 gctatctcct cttcttcaag cggggcgccg gcgtcaccgg ccacgcagca cgccccctgg
     2641 caccgcgcca ggtggcagca gaacctgacc aggtgcaggt cgtcggaaac tgcggtgtcg
     2701 ccgatgagga tcatctctcc tggttaaatt tccagttgat gcggctgctc agcaggtcga
     2761 tcgccacgct gttctctcct ccctgcggga tgatcaggtc ggcatacctt ttcgatggct
     2821 cgatgaactg cagatgcatc ggtttgacat acgtctcgta gtggcgcagc accatggcga
     2881 catcacggcc gcgctccagg atgtcgcgct ggatgatgcg ggtcaggcgg tcgtcggcat
     2941 cggcatccac aaataccttc acattgaacc ggttacgcag ttccgggtgg gtgaggatca
     3001 ggatgccctc gacgatgatc acccgcttgg gctcaatctt aaccgtttct ttcaagcggg
     3061 cacattccac gtaagaatag cgcggcatct caatggaatg cccttttatc agcttatcga
     3121 ggtgatgaat gagcagctcc cattcgatcg aatcgggatg gtcgaagttg atcagctttt
     3181 tctcctccgg cgacaaatgg ccgttgtccc agtagtaggc gtcctgcgac atgatggaaa
     3241 ccgactttgg cggcaagccc tcgatgatct tgttgacaac ggtggttttc cccgaccccg
     3301 atccaccggc gattcctatg acgagcatgg tgctgatttt tttgctaaaa tacaaaaagt
     3361 tggcagcatg tcctgaaaag cttatcggcg agggatcatc gccgacatat gatcaatata
     3421 ataatgacgc gaacgcagtg agcttatggt gttcgcgtag cgaactaatg accaccttct
     3481 gacattatcg cactttatat tatttttact gaataaataa ttcctttcag aatgaagttg
     3541 ctactatcac ttacactttt cttttcaata gttgttgaat caatcgccca ggttccgctg
     3601 aactggaccc gcgatgagat caaccccggg gaggacttca cgcttttcca ggataattcg
     3661 gtattcaccg aaggcgagaa atcgctgcac atgcagttga acaaaggttc ggtgccctac
     3721 cttgtatcgg atatctttta catcagcccc ggcgccgatt atactttttc ggtcgatgtc
     3781 ctggataacg atacggccgg ccaggtgaag atctatgccg atttctacga tgcctacgga
     3841 tttgatattt tcggcgaacc gcccgtgttt tcagcggatt cggccgattg ggtgacgatt
     3901 tcctggcagg ggaccgtccc gagccaggcc gttgtcggtt acatcctcat caaattctat
     3961 aatcaaccta atctttatac atttactaaa caggcaaata tttggctgga taacgtcagg
     4021 tttatccagg agggcggccc gaacctggtg gcaaacggcg gatttgaaga atgggcggtc
     4081 ggtatggatg atgatcggga agaaatggag ttgactgttt ttccaaatcc ggccgatgat
     4141 tttatcggtt taaaattcac agataaaatt aaccgcgtcg aattgtataa cagcgccgga
     4201 caacgggtga ggcttcagga ggctaatggc acagtggccg gaatccctgt tgcaggcctg
     4261 atggagggac tatatcttct taagattgag accggcatcg gtgtcgttgt gaagaaactg
     4321 atcatccatc attaattaat atttaacaga ccaaaaggaa atatctgtga attttgtgtc
     4381 ctcaatctgt gaaatctgtg agaaacaata atcatcaact atgaacaaaa caaaatttat
     4441 aatcattctt atctcagggg tactgatcct cttcacatcc tgcaaccaac aaccgccaca
     4501 acaagaaaag gcgcagaatg agttcacctt cgcctttctg accgatattc acgtgcagcg
     4561 ggagaaagac gctatttcgg gcttccggaa ggccattaca aaggtcaatg aattgaagcc
     4621 cgatttcgtg atcacgggcg gcgacctggt aatggatgct ttgggccaga cctatgagag
     4681 ggctgattcc ctgtatcata tctacacaac gatgatgacc gagttcaata tgcctgtttt
     4741 caatacgatg gggaaccatg aaatatacgg ctggtatgtt ttgaaagatg gcgatacaaa
     4801 tcatcccgag tacggcaaga aaatgtttga aaacaggatc ggaccgagat ttcagcggat
     4861 cgaccgcttt ggatggatca tatttatcct ggattcggta gtaaaagaca gcaccggcga
     4921 cggctatgag ggtggcgtcg atg
//
LOCUS       DAOPNH010000257         4913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_19991_length_4913_cov_6.493001, whole genome shotgun sequence.
ACCESSION   DAOPNH010000257 DAOPNH010000000
VERSION     DAOPNH010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19991_length_4913_cov_6.493001"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..820
                     /locus_tag="PKZ74_11985"
     CDS             164..820
                     /locus_tag="PKZ74_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93518.1"
                     /translation="MKRIAFLIAGLVAFTMVNAQTETNENAGEINTVFGKNGRPAVGW
                     FIGLDHGYTQFESRDVWTGGLNFGMIIDHKFSIGLTGTGWTNRNGMYYENISGDDGAY
                     LEGGYGGLLLEYTLFPKSLVHLTIPVTIGGGGVSYISESDIPVWDEDEWDFDNVVIDD
                     DLFFVVEPGIRAEMNVFRFMRLNAGVSYRHTEGLQMINTSEDLMNNFTATVGLKFGKF
                     "
     gene            920..2638
                     /gene="sulP"
                     /locus_tag="PKZ74_11990"
     CDS             920..2638
                     /gene="sulP"
                     /locus_tag="PKZ74_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HPM93519.1"
                     /translation="MSYWFLMNAQFKNLFPVYTWLPGYRISFLKWDLIAGTTLAFFAI
                     PTSMAYATLAGLPPQTGIYCYLFAGLLYFVFGTSRHLAVGPTSAISIIVATSIGALAG
                     NDPAKAITLASATALLMAGLFLGAYIIRLSSLINFISDTVLTGFKAGAALVIASTQLP
                     KLFGIPDAGDNFIDRIRNLITNLGETNITVLVFGMIALILLLLGNHYLKGKPVSLIIV
                     IISILLVSFAGFKEFGITVVGEIPKGIPALRWSVPNSHEFNDIFFLALACFFLSYIES
                     ISAARSIAREKGYEIDPQQELLALGAANLASSLGGGYAVAGGLSQSTVNARSGAKSLI
                     SLIITSVILAVSLYFLTGLFKNLPHVILAVIVIDALISLVNLKELKHLFRVSRLEFWV
                     SALTIVAVVLFGVLKGILIAVILSIISVLRKSASPHMAVLGRIPGTTLFSDMLRHPDN
                     QPVDGVLILRPESSLLYFNVNFIRDSIKQALSNYPGKPYLVILDLSSANFVDVAGARF
                     LLWLEDDLEKKGIGFRIVDALGNVRDTLRKAGMEKEIGHISRKHTINEILAEYNGKPV
                     TEEESF"
     gene            2828..3811
                     /locus_tag="PKZ74_11995"
     CDS             2828..3811
                     /locus_tag="PKZ74_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93520.1"
                     /translation="MKTAKLFFFVICILAWAGINETFAQWGVNGSHIYNTNAGNVGIG
                     NNSPTTLLHVQKLMTEPTITIQNLGGTGGATYTMIDNASGANWKFKATLSGGFKIRDH
                     ANSLDVVVIEPNSAANSLYINSSGFIGIGTNAPAQKLTVFGGDFKIDDYYPFIFLNNT
                     STGGNMGITFWQAGEYRAWLYYDDGESLLRLNAEQGGGSRNDLVILNSGSVCLGTTTE
                     ATGYRLNVDGKVICTEVRVEALANWPDYVFGDNYRLMTLAELEQNIKSNGHLPGLPSA
                     SHVEANGFELGNMQRILLEKIEELTLYTIEQNKQITELQKKVSDLEKAGRK"
     gene            3808..4164
                     /locus_tag="PKZ74_12000"
     CDS             3808..4164
                     /locus_tag="PKZ74_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93521.1"
                     /translation="MKKFKYILLTTMVMGFLFVAGQQIPSTTPVDETGPVIDKNAQAT
                     PDKALTLPGRAAPEAEVPIGTSDFRDETMPPDIIPSTKTPVKNGQELREDDQKQPSGP
                     DEATGPVEVKPDKKQP"
     gene            4175..>4913
                     /locus_tag="PKZ74_12005"
     CDS             4175..>4913
                     /locus_tag="PKZ74_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93522.1"
                     /translation="MKTIKTMLILVLGLTITGYLFGQENPWELWKNPGTDDFATIQQN
                     VENYYADKDKFVRGSGYKQWKRWEYLQRNRLTDDGKIFNYTAKNFQEYHDYLSQKGAG
                     RDITTTYGYWESLGPTYFVDGNGWNGGIGRVNCITFHPSNASVIWVGCPSGGLWRTDN
                     GGTSWTPLTDGMPRIGVSGLAVNYNNTNIMYLLTGDGDGADVYSIGVLKTIDGGVTWN
                     STGLTWAPTDFEVGFKILMHPTNPAILF"
BASE COUNT         1273 a         1153 c         1218 g         1269 t
ORIGIN      
        1 tttcactatt ccctattcac tattcctttt ctaaacctca ccggctggat tccgcagttg
       61 gcgggtattt tgtttcgcag gtagagagcg gcgtgtactt tcgcatcatc aaatttaatt
      121 aatcacaatc aacattatta atcacaatta aaaactaagt acaatgaaaa gaattgcttt
      181 tttaatcgcg ggtctggttg ctttcacaat ggtaaatgcg cagacggaaa ctaatgaaaa
      241 cgccggtgag atcaatacgg tgttcggtaa gaacggcagg ccggcagtcg gctggtttat
      301 cggcctggat catgggtata cccaattcga aagccgggat gtatggacag gtggattgaa
      361 ttttggtatg ataatcgacc acaaattttc gatcggcctg accggtaccg gatggaccaa
      421 ccgtaacgga atgtattacg agaatatttc gggcgacgac ggggcttacc ttgaaggagg
      481 ttacggcgga ttgttactgg aatacaccct gtttcccaaa tcgctggtgc acctgaccat
      541 cccggtcacg atcggtggcg gcggtgtttc ctatatttca gaatccgaca tcccggtatg
      601 ggatgaagat gagtgggatt tcgacaatgt ggtgatcgac gacgacctgt ttttcgtggt
      661 tgaaccggga atccgcgccg agatgaacgt tttccgcttc atgcggctga atgcaggggt
      721 aagctacagg cataccgaag ggttgcagat gatcaatacg tcagaggacc tgatgaacaa
      781 cttcaccgca acggtggggc tgaagttcgg gaaattctga cggattgtga ttaatgatga
      841 aagggcggtg ccgaaagggc cgcccttttt gcgttttaag gtttgagagg atttcggaga
      901 aagtgtttaa atttgacata tgagttattg gttcctgatg aatgcgcaat tcaaaaatct
      961 tttccctgta tatacctggc tgccagggta ccggatatcc tttctgaaat gggatctcat
     1021 tgccgggacg acactggcat tcttcgccat ccctacttct atggcttatg cgacccttgc
     1081 cggtctcccg ccacagaccg ggatctattg ctacctcttc gccggcttgc tctattttgt
     1141 cttcggcacc tctcgccacc tggctgtcgg gcccacttca gccatctcca tcatcgtggc
     1201 aacttctatt ggcgctttgg cagggaatga cccggcaaag gcgatcacgc ttgcctctgc
     1261 gacagcactc ctgatggcag gactgttcct gggtgcttat attatcaggt tgagctcgct
     1321 gatcaacttt atcagcgata cggtactgac aggatttaag gcaggtgccg ccctcgttat
     1381 cgcttcaacc caattgccaa aattattcgg aataccggat gccggcgata attttatcga
     1441 caggatccgg aacctcataa caaatctggg ggaaacaaat atcacggttc tggttttcgg
     1501 catgatcgcc ttaatccttc tccttctggg taatcactat ctgaaaggca aacccgtttc
     1561 gttgatcatt gtgattatat ccattctatt ggtttctttt gctggattta aggaatttgg
     1621 gattactgtt gttggtgaaa taccgaaagg catcccagca ttgagatggt cagtgccaaa
     1681 tagccatgaa tttaacgaca ttttttttct ggcgctggcc tgttttttcc tgtcatatat
     1741 agagagcata tccgccgccc ggtctattgc aagggagaaa ggctatgaga tcgatccgca
     1801 gcaggaattg ctggccctgg gagccgctaa ccttgcatca tcattgggag gtgggtatgc
     1861 tgtggccggc ggactttcgc aatccacggt caatgcccgg tcgggtgcca aaagcctgat
     1921 ttcgcttatt attacctctg taatactagc tgtaagcctc tatttcctga cgggcctgtt
     1981 taaaaacctg ccccacgtga tcctggccgt tatcgtgatc gacgcgctga tcagcctggt
     2041 caacctgaag gagttgaaac acctgttcag ggtgagccgg cttgaattct gggtgtcggc
     2101 gctgaccatc gtggcggttg tcctctttgg cgtgctgaaa gggattctta tagcggtgat
     2161 cctgtctatc atctccgttt tgaggaaaag tgcctccccg cacatggcgg tattgggccg
     2221 gatacccgga acaacacttt tttctgacat gcttcgccat ccggataatc agccggtcga
     2281 cggagtgctg atcctccgcc ccgaatcatc acttctttac tttaatgtga attttatcag
     2341 ggatagtatt aagcaagcgt tatcaaacta ccccggcaag ccatatcttg tcatcctcga
     2401 cctctcttcg gccaattttg tcgatgtggc aggcgcccgg ttcctcctct ggctggaaga
     2461 tgacctggaa aagaaaggga tcggtttccg gatcgtcgac gccctgggta atgtgcggga
     2521 taccctgcgt aaagcaggca tggagaaaga gatcggccat atcagccgga aacataccat
     2581 taacgagata ctggctgaat acaatggcaa accggttacg gaggaagaga gcttttagca
     2641 gttattgtct ggcattgtaa atcatttgaa tttccctcgc tgaaagttcc tggtaatatc
     2701 tctgaaaatc tcgatttcac ttgacttttt atccgattgt acgtatattt acgtactatt
     2761 gaccaattcc ccccgcaagt ccctttaatg attgattttg aggatataac aaccaaatac
     2821 tatcattatg aaaacagcaa aacttttctt ttttgtcatt tgcatactgg catgggccgg
     2881 tatcaacgaa accttcgccc agtggggcgt taacggcagc catatttata acaccaacgc
     2941 cggcaatgtt ggtatcggga ataattcccc gacgacactg ctgcatgttc agaaacttat
     3001 gaccgaaccg acgattacca ttcagaacct gggcggtacc ggcggtgcta cttacaccat
     3061 gatcgacaat gccagcggcg ccaactggaa attcaaagct accctgagcg ggggatttaa
     3121 aatcagggac catgccaact cactggatgt ggttgtcata gagcctaaca gtgcagccaa
     3181 ttcattgtat attaacagtt caggatttat aggaataggg actaatgcac ccgctcaaaa
     3241 acttacagta tttggaggcg attttaaaat tgacgattat tacccgttca tattcttaaa
     3301 taataccagt accgggggaa atatggggat tacattctgg caggctggag aatatcgtgc
     3361 atggttatac tatgatgacg gtgagagttt gttgcgactg aatgcggagc agggcggagg
     3421 atcaagaaat gacctggtta ttttaaacag cggttcagtt tgcctgggaa ctactacaga
     3481 agcaaccggc tacaggctga atgtggacgg caaagtcatc tgcacagaag tcagggtgga
     3541 agcactggcc aactggcccg attatgtatt tggtgacaat taccgactga tgaccctggc
     3601 tgaattggaa caaaatatta aatcaaacgg acacctgccc ggcctgcctt ctgcctctca
     3661 cgtggaagcc aatggttttg aactgggcaa catgcagagg atcttactgg agaaaatcga
     3721 agaacttacc ctctatacca tcgaacaaaa taagcaaata accgaacttc agaagaaagt
     3781 cagcgatctt gaaaaagccg gaagaaaatg aaaaaattta aatacatatt attgaccacg
     3841 atggtgatgg gattcttgtt tgtcgccgga caacaaatac cttcaaccac gccggtggat
     3901 gagaccggtc cggtcatcga caagaatgca caggcaacgc ctgacaaagc attgactctt
     3961 cccggtcgtg ctgcgccgga agcagaggta cctattggca catccgattt tcgcgatgag
     4021 accatgcctc cggatattat cccatcgaca aaaacccctg ttaaaaatgg acaggaactc
     4081 cgggaggacg accagaaaca accctcaggc cctgatgaag ctaccggtcc tgttgaagtt
     4141 aagcctgata aaaagcaacc ttaaagccat ggatatgaaa acgataaaaa ctatgttgat
     4201 cctggttctg ggtttaacca tcaccggtta tttatttggc caggaaaatc cctgggaact
     4261 ctggaaaaat cccgggacgg acgattttgc caccatacag caaaatgtcg aaaactacta
     4321 tgctgacaag gataaatttg tacgcggttc gggttacaaa caatggaaac gctgggaata
     4381 cctgcaacgc aaccgcctga ccgacgacgg gaaaatattc aattatacag ctaaaaattt
     4441 ccaggagtat catgattatc tgagccagaa gggagcaggc cgtgatatca cgaccactta
     4501 cggttactgg gaatcgctcg ggcctaccta ttttgtagat ggaaacggct ggaacggggg
     4561 aataggacgt gtcaactgta ttacgtttca cccctccaat gccagtgtga tctgggtcgg
     4621 atgcccttcg ggcggattgt ggagaacgga taacggcgga acttcctgga cccccctgac
     4681 cgacggcatg ccccggatcg gcgtgtcggg actggctgtc aactacaaca atacgaacat
     4741 catgtacttg ctgaccggag atggcgacgg ggcagatgtc tattccatcg gcgtcctcaa
     4801 aactatcgat gggggcgtta cctggaacag tacgggattg acctgggcgc ccaccgattt
     4861 tgaggtcggc tttaagatcc tcatgcaccc gacaaacccg gccatcttgt ttg
//
LOCUS       DAOPNH010000258         4833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_20452_length_4833_cov_6.061532, whole genome shotgun sequence.
ACCESSION   DAOPNH010000258 DAOPNH010000000
VERSION     DAOPNH010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20452_length_4833_cov_6.061532"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..2544
                     /locus_tag="PKZ74_12010"
     CDS             367..2544
                     /locus_tag="PKZ74_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM93523.1"
                     /translation="MKNCFKVMIISGLACMFSLHAVSQDIWKAINDGDAVKTREWISK
                     DPALLNQRNADLLTPLNLAASLGQKEIAELLLDLGADPAIGDNENSQPIHLAAIYGHV
                     PVVELLLARGNPINTLDDNGMTPLLFAASRGQLDASRFLVEKGADTKIVSSRGINALQ
                     MAVIGGNAELVKYFISKGFSVNSAVRDGLTVLHTAASFGRTEIVKYLVEKGARIDAES
                     EEGAQPLALAVGRNSYDAAQFLISKGVEVGHRDKQGFAAIHEAAGRGNLGVVQLLLDH
                     GADINSLSDAGWTPLTNAAWAPTAAEIGRFLIQHGASVNPDPCREVKSCTCGPNFNTP
                     LHQACAMNKADLVKILIDNGAKVNLLNREGWPPMFSAVRSGNAEVVAYLINHGAFLNV
                     KDPRTGGTELHMAAALGFGEVAGMLIDRGADFKAKNNDGLTPVDFALNYGQNMIAYRM
                     LAAGAADSSLASYFGKECLLAKKYNAGEAEVWFLGHSGWAIKTSSHFLVFDYFDNPRS
                     QKPDNPCLSSGFITDEALAVQNTVVFSSHAHQDHYNRSIFNWDDNNDNVKYVLCHKPA
                     DADNPYTYIPVDESAEVAGMKIQVIRSTDSGGGFLVEVDGLVIFYMGDHSNGNDELMP
                     EFTAEIDKVAQLNKDIDLMFGPIRGCSLGTPEQVKAGTYYALEKLHPALFIPMHSGDY
                     TFEYRNFTEQAHNDGVDTPMKPVFAKGDRFHYLKGEPVAEVYQ"
     gene            2686..3390
                     /locus_tag="PKZ74_12015"
     CDS             2686..3390
                     /locus_tag="PKZ74_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93524.1"
                     /translation="MKKVLLSISIMVIASMAFTQQNQPSPFGNGGKETMNGISYAPTV
                     TCDNEKGYYDFPFTENGITVTGSGTGTYTVYQPGWGSCGIFAAANCVWIGTDGPATYT
                     NTFSSPVNNMIYNMTATDIGEVITITTDAGTPSITYTDGTCPSWFNISGNTITCVDGY
                     YPAAGGRFLITSTQDFSSITFSHNGTMNGTTMTMCFDQAITPPPPSAIPVSNWALILG
                     GALIATFVFVRFRRMI"
     gene            complement(3591..4247)
                     /locus_tag="PKZ74_12020"
     CDS             complement(3591..4247)
                     /locus_tag="PKZ74_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93525.1"
                     /translation="MKKIIFVLTLVSLTALNYKGFSQVSERMDSIYRNKEANLPAWTF
                     NFNPVGVALYGPIIQMEFKISNRGYLVPWLRYSYAGVVSTYNFTNFEDDSKYSPMSSA
                     LGIGYKKFIPGDVSRQHIYWGFFGEIMYDECLQNIGSTAGEEFNQTRIAAAAYLNVGY
                     RWKSRRNFYWSLGILPGYVYDLKHEGTFTNSGGPVQQDFKGHKLTGMIDFSMGWNFER
                     "
     gene            4619..4793
                     /locus_tag="PKZ74_12025"
     tRNA            4619..4793
                     /locus_tag="PKZ74_12025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4754..4756,aa:Pro,seq:ggg)
     assembly_gap    4643..4742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1182 a         1106 c         1195 g         1250 t
ORIGIN      
        1 ttctgcacat tcggagtgta ataacacggt atgcagcaag gcagtaaaat cgtgataatt
       61 aatatccggg taatgttgaa aagacctgag gtattgtagc acaacgttcg ccgtttcaat
      121 ttattacctc tgagcttcct gacaggatca tcggttttca tcggatctgc gtttaaaggt
      181 ttaacgcgtt tgttaaatgg caattaccgt aaagatacga ataaatttaa acgttgtcaa
      241 ttcgattctt catcccggga cgccaattgc aaaccgatcc gatcattctg taaatctggt
      301 cgttaaaaat ctgtttaccg ggtataaatt gaacgcaaat tcacctctcc taaagaaaaa
      361 gtacttatga aaaactgttt taaagtaatg atcatttcag gtttagcctg catgttctcc
      421 cttcatgccg tgtcgcaaga catctggaaa gccattaatg acggtgatgc cgtaaaaacc
      481 cgcgaatgga tttctaaaga tccggcactt ctcaaccagc gcaatgccga cctgctgacg
      541 ccgttgaacc tggccgcatc actggggcaa aaggaaatcg ctgaattgct cctggatctc
      601 ggcgccgatc cggccattgg tgataatgag aattcgcagc ctattcacct ggccgcaatt
      661 tatggccatg ttcctgtcgt tgaactgctc ctggcccggg gcaatccaat caataccctg
      721 gatgataacg ggatgacgcc gctgttgttt gccgccagcc gggggcaact ggatgcttcc
      781 cgcttcctgg tagaaaaagg ggccgacacg aaaatagtga gttcccgggg gatcaacgcg
      841 cttcagatgg cggtgatcgg gggaaatgca gagctggtta aatattttat cagcaaaggg
      901 ttctctgtca attccgctgt ccgcgacggc ctgacggtac tgcacacggc cgcttcgttc
      961 ggacggaccg aaattgtcaa atacctggta gagaaagggg ccaggattga tgcggagtcg
     1021 gaggaaggcg cacagccgct ggcattggct gtcggccgga attcttacga tgccgcccaa
     1081 ttcctgattt caaagggtgt ggaggtcggt catcgcgaca agcagggctt tgcggccatt
     1141 cacgaagccg ccggaagggg aaacctgggt gtggtgcagc tcctccttga tcatggcgcc
     1201 gatatcaaca gcctgtctga tgccggctgg actccgctca ccaatgcagc ctgggcgccc
     1261 accgccgctg aaattggccg cttcctgatc cagcatgggg cttccgtcaa ccccgatcca
     1321 tgccgggaag tcaaatcatg cacctgcggg ccgaacttca acaccccgct gcatcaggct
     1381 tgtgccatga ataaagccga cctggtgaag atactgatag ataatggcgc caaagtaaac
     1441 ctgctgaacc gtgagggttg gccaccaatg ttttctgccg tcaggagcgg aaatgctgag
     1501 gtcgtcgctt acctgatcaa tcacggtgcg ttcctgaatg ttaaggatcc ccgtacggga
     1561 ggcaccgaat tgcatatggc tgcagccttg ggctttggcg aggtagccgg catgctgata
     1621 gaccggggcg ccgatttcaa agcgaaaaac aatgatggcc tgacgccggt cgattttgcc
     1681 ctgaattacg gccagaacat gattgcctac cggatgctgg ctgccggcgc cgctgacagc
     1741 agcctggctt cttacttcgg caaagagtgt ttactggcta aaaaatacaa tgccggcgaa
     1801 gcggaggtgt ggttcctggg ccacagtggc tgggccatta aaaccagcag tcatttcctg
     1861 gtattcgatt atttcgataa tccccgcagc cagaaaccgg ataatccctg cctgtcatcg
     1921 ggttttatta cggatgaagc gcttgcagtg cagaatacgg tggttttcag ctcccatgcc
     1981 caccaggacc attacaatcg ttctatcttc aactgggacg acaacaacga taacgtgaaa
     2041 tatgtccttt gccacaaacc agccgatgcg gataatcctt atacatacat cccggtcgat
     2101 gagagcgctg aagttgccgg tatgaaaatc caggtgatcc gttcgaccga ctcgggaggt
     2161 ggttttctcg tggaggtcga cgggctggtc attttctata tgggagatca cagtaatgga
     2221 aacgatgagc tgatgccgga atttacggca gagatcgaca aagtggcgca actgaacaag
     2281 gatatcgacc tgatgttcgg gccgatccgt gggtgcagcc tcggaacacc cgaacaggtt
     2341 aaggcgggga cctattatgc cctcgaaaaa ctgcatcccg ctttgttcat tccgatgcat
     2401 tcgggcgatt atacattcga atatcggaat tttacggaac aggcgcacaa cgatggtgtc
     2461 gatactccga tgaaacctgt ttttgccaaa ggcgaccggt ttcattattt aaaaggtgaa
     2521 cccgtcgcgg aagtatatca gtaaggcctg cgtttagtta gaatttaatt atttagaaat
     2581 ttcgtgtacg aatggacgct taaagcgctt attcgtacac gatttatatt gaagaaaaga
     2641 tagaatcttt tttctttgta cgttgaaact cataatctga aacatatgaa aaaagtctta
     2701 ctaagtattt caattatggt aatcgcttcg atggctttta cccagcagaa tcaaccctct
     2761 ccttttggaa atggcggcaa agaaacgatg aatggaatca gctacgctcc tacagttacg
     2821 tgtgacaatg aaaagggcta ttatgatttt ccattcaccg aaaatgggat tacggttaca
     2881 ggttcaggaa caggtactta caccgtctat caaccgggtt ggggatcgtg tgggattttt
     2941 gctgcagcca attgtgtctg gatcggcact gatgggcctg ctacttatac gaatactttt
     3001 tcttcgccgg ttaataacat gatttacaac atgactgcta ctgacattgg cgaagtgatt
     3061 accatcacaa cggatgcagg aacaccgtcc atcacctaca cagatggaac atgtcccagt
     3121 tggtttaaca tctccggcaa taccattacg tgtgtggatg gttattaccc tgcagccggt
     3181 ggccgtttcc tgattacttc cactcaggat ttttcatcga ttacattttc acacaatggg
     3241 acgatgaatg gaacaaccat gaccatgtgc ttcgaccagg ctatcacccc acctcctcca
     3301 tctgcaatac ccgtatccaa ctgggccctc attctgggcg gcgcactgat tgccactttt
     3361 gtttttgtaa gatttcgtag gatgatttaa tgataattag tttttagaaa agaggctgct
     3421 cagataatga gacagccttt ttttttaatg gtagtccgga gggtgaatcc gtcggttcaa
     3481 caagggttct tattccgacg agcgtccagt ctgttccatc tttgtaatat tcccggaacc
     3541 aacagcttta gtcgttggat cacgagcttt tttcaagcaa tcaaatcgaa ttatcgttcg
     3601 aagttccagc ccatcgagaa atcgatcatg ccggtcagtt tgtggccctt gaaatcttgc
     3661 tgcaccggtc cgccgctgtt ggtaaaggtt ccctcgtgtt tcaggtcgta cacatagccg
     3721 ggcagaatgc ccaggctcca ataaaaattc cttctcgatt tccagcggta gcccacattg
     3781 aggtaagcgg cagcggctat ccgcgtttga ttgaattctt cgccggcagt cgaaccgata
     3841 ttctgaaggc attcatcgta catgatctcg ccaaagaaac cccagtagat atgctgccgg
     3901 ctcacatcgc cgggaataaa cttcttgtat cctatgccca gcgcgctact catcgggctg
     3961 tacttgctgt cgtcctcaaa attggtgaaa ttgtaagtgc tgaccacgcc ggcgtagctg
     4021 tacctaagcc aggggacgag atacccgcgg ttggaaatct tgaattccat ctggatgatc
     4081 ggcccgtaaa gagcgacgcc gacaggattg aagttgaatg tccaggccgg gagattcgct
     4141 tctttgttgc ggtaaatcga gtccatcctt tcagaaacct gagaaaagcc tttatagttg
     4201 agggcagtta gtgagaccag tgtcagaaca aatattattt ttttcatttt ggctgataaa
     4261 caatagttca aggctgtttt caaatgagcc gttaaagtta tggattaatc attagtacga
     4321 aattgttttt cataaaaatt tcgtattttt gcactccttt tgatttcaaa tttcagttat
     4381 tgtaaggaat ttggtgtttg gaatttggtg cttggaattt ggaattcgga atttaacatc
     4441 gcggggtgtg gcgcagcctg gttagcgtgt cccgactgag tcgggaaggt cgtgagttca
     4501 aagtggccat cgttctttta attagattac ggggtgtggc gcagcctggt tagcgtgtcc
     4561 cgacggaagt cgggaaggtc gtgagtccaa agtggccatc gttcttttaa ttagattacg
     4621 gggtgtggcg cagcctggtt agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nncgtgcttg actgggggtc aagaggtcgt gagttcaaat ctcaccactc cgacaggtca
     4801 attggacaat aggcagttgg acaacttgtc ccg
//
LOCUS       DAOPNH010000259         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_20458_length_4832_cov_8.059452, whole genome shotgun sequence.
ACCESSION   DAOPNH010000259 DAOPNH010000000
VERSION     DAOPNH010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20458_length_4832_cov_8.059452"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKZ74_12030"
     CDS             <1..383
                     /locus_tag="PKZ74_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPM93526.1"
                     /translation="DAFIIGGSTTGADNWESAHKTRWSDFFAKLEKAEIKGKPFALYG
                     LGNQVLYPNHFVDGMAFLKEKFEKRGAVLKGRWPVDGYDFEESESVENGLFYGLALDF
                     DTQDELTDERIAKWTGQIRKEFGI"
     gene            complement(387..1862)
                     /locus_tag="PKZ74_12035"
     CDS             complement(387..1862)
                     /locus_tag="PKZ74_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPM93527.1"
                     /translation="MILSKKIITFLKLFSSFFPLFLITIFFTSCLTDHQGKIKPIRAG
                     EDSSRKLRIHEIRERGKLIATTNYNSTDYFIYRGEPMGYQYEKLKMFADYLDVELEIK
                     VALNLDEAFTSLEEGKADVIAMGLAQTRDRVEKYDFSSPIFQTRQMLVQRKPDNWRKM
                     RTWDDIEKSLIRNPLDLAGKTVIVQKSSAYAGRLENLSEEIGAPINVQVDPLMEAEQL
                     VEAVAKGEIDFTVCDEHMAAFFERQHSELDIRTPISFPQNIGWVVRHDSDSLLAAIND
                     WLGESESTLASRHLMDKYFNNPRASFMARSEKINMGGKQISEYDEILRNISRQHDFDW
                     RLVASLVYQESQFQPEVKSWAGAFGLMQLMPATAKLFGIDSTASEFQQIEAGVMFMKK
                     IDDELPRDIKDRSERIKFILAAYNVGIAHVMDARRLAEKHGKDPNVWTGNVDYYILNK
                     SNPKYYRDSVVRYGYARGEETYQFVNEVLDRFEHYKNYVGD"
     gene            complement(2038..4524)
                     /locus_tag="PKZ74_12040"
     CDS             complement(2038..4524)
                     /locus_tag="PKZ74_12040"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPM93528.1"
                     /translation="MTKFYKQKLLLLLLMFGPVGLVLAQDFLPTMSDNYMGINQVVLQ
                     PASISDSRFMVDINVFGANSDIYNDALRLRSKWILNPSGYRNNENWWSENTYLDNPDG
                     LDKHMFSSQSILGPGFMISLPYSQAIGFTSRVRSITNVDGISEPLIRTVQSDYTNENY
                     WNQWVFDDHMRAVQHVFADYGLTYAATILNTGPHVLKGGVTVKLLQGIAASYIQSDSL
                     YYYFDGENNDGDYPMSYNASHIYSGVSDNWGDDNEAGIFDYSMNFQYTSKPSAGLDVG
                     FVYEFRPKYLDFRYDMDGEKDLERNDKNKYLLKVGASIVDIGRIKYKKEYNSFDFSAN
                     FTPDYADRYQNGDNSVPDNTYWLNAEVGSFSFQKYVNFVDTMYHRQLSGRGVGKVDGN
                     DEKFMVTLPTALSLQIDVRVVEGFYVNLTSFSGLHQGFSRAPNSHQMSNYSITPRYER
                     KWFTISMPFQYNQFQKMNVGLGFRAGFIYAGVTNLFSGLIKDPYGTNIYLGAKIPLFK
                     KGSPPDKDNDGVSDEKDICPDIPGPWQFYGCPDRNGDGVADSEDACPDIAGKKEFLGC
                     PDTDGDGVSDKDDKCPKVPGPKITNGCPDFDNDGVVDDQDECPEIAGPAKFAGCPDSD
                     GDGIPDIKDNCPDLAGLAEYAGCPYLDTDGDGVKDADDMCPDKKGPPENNGCPYSDSD
                     GDGVVDKEDRCPLTPGDPANFGCPILAVEDAEILNTAFQNLEFETAKAVIRSSSFPSM
                     DELARLLISKPSYKLKISGHTDNVGSDAYNMTLSKNRAQSTARYLIGKGVPQNRLLTD
                     WFGESQPIADNNTPEGRQINRRVEMQVVFD"
BASE COUNT         1248 a         1158 c         1135 g         1291 t
ORIGIN      
        1 acgacgcttt tatcatcggg ggctcaacca ccggcgccga caactgggaa agcgcccaca
       61 aaacgcgctg gagcgacttc ttcgccaaac tggaaaaagc ggaaatcaaa gggaaacctt
      121 tcgccctcta tggcctgggt aaccaggtcc tctacccgaa ccactttgtc gacgggatgg
      181 cgttcctgaa agagaaattc gagaaaagag gcgccgtgct gaaaggccgc tggcctgtcg
      241 atggttatga tttcgaagaa tcggaatcgg tcgagaacgg attgttttac ggcctggcac
      301 tcgatttcga cacgcaggat gaacttacgg acgaacggat cgccaaatgg accggccaga
      361 tccgtaaaga atttggcatc tagcggttaa tcgccgacgt agttcttgta atgctcgaag
      421 cggtcgagca cctcgttcac aaactgatac gtttcttcac cgcgggcata gccgtatcgt
      481 accaccgaat cgcggtaata tttcgggttc gacttattga ggatataata atcgacgtta
      541 cccgtccata cattcgggtc tttcccgtgt ttttccgcca gcctgcgcgc atccatcaca
      601 tgggcgatac ccacattgta ggccgccagg atgaatttga ttcgctcgga gcgatcttta
      661 atgtcgcgcg ggagctcgtc gtcgatcttt ttcatgaaca tcaccccggc ttcgatctgc
      721 tgaaactcgg aggcggtgct gtcgattccg aatagctttg ccgtagccgg catgagctgc
      781 atcaggccga aagcgccggc ccaggatttg acctcgggct ggaactgcga ctcctggtaa
      841 accagcgagg ccaccaggcg ccagtcgaaa tcgtgctggc gggagatatt gcgcaaaatc
      901 tcgtcgtatt cggagatctg tttcccgccc atgttgattt tctcgctcct ggccataaag
      961 gaagcacgcg gattgttgaa atacttatcc atcaaatgcc gtgatgccag tgtgctttcc
     1021 gattcgccca gccagtcgtt gatcgcggcc aggaggctgt cggagtcatg cctgaccacc
     1081 catccgatat tctgcggaaa gctgatcggg gtccggatat cgagttccga atgctgccgc
     1141 tcaaaaaagg cggccatatg ctcgtcgcaa acggtgaagt cgatctctcc ctttgcgacg
     1201 gcctcgacaa gctgttcggc ctccatcagc gggtcgacct gaacgttgat cggcgcaccg
     1261 atctcttccg agaggttttc gagacggccg gcataggcgg aagatttttg cacgatgacg
     1321 gttttaccgg cgagatccag cgggttgcgg atcagcgatt tctcgatatc gtcccaggtg
     1381 cgcattttac gccagttgtc gggtttgcgc tgcaccagca tctgccgggt ctggaaaatc
     1441 ggtgatgaaa aatcgtattt ctcgacgcga tcgcgggttt gggccaggcc catggcaatg
     1501 acgtcggctt tgccttcttc caggctggta aatgcttcgt cgaggttaag cgcgactttt
     1561 atctccagtt ccacatcgag gtaatcggca aacatcttca gcttttcgta ctggtatccc
     1621 atcggttcac cgcggtagat aaagtagtcg gtagagttat agttggtcgt ggcaatcagc
     1681 tttccacgct ccctgatctc gtgaatacga agcttacgcg acgagtcttc tccggctcgt
     1741 atcggtttta tttttccctg gtgatcagtc aggcaggaag taaagaagat tgtgattaaa
     1801 aatagaggga aaaaagagga aaaaagcttt aagaaggtga ttattttttt agataagatc
     1861 atctattctg ttttcgttca acactggcct tccgttaaat ggcactgcaa gcgcctctgg
     1921 atcaggcttt tatttaaaga tattaagtta ttggattagc gtcggcaaaa gtaagtaaaa
     1981 attccatccg gtttacatcc ggatggaatc ttatgaatat cagtttgtta aaaaatgtta
     2041 atcaaacacc acctgcatct caacccttcg gttgatctga cggccttcag gtgtattatt
     2101 atcggctatt ggctggcttt caccaaacca atcggtgaga agtctgtttt gcggcactcc
     2161 tttgccgata agataacggg cggtcgactg cgcccggttt ttcgacaggg tcatgttata
     2221 cgcatcgctt ccgacgttat cagtatgtcc tgaaatcttc agtttatagg atggtttgct
     2281 gataagcagt ctggcaagct catccattga ggggaatgaa gaagagcgga tcactgcttt
     2341 tgccgtttca aattccaggt tttgaaaagc ggtattcagg atttcagcat cttcaacggc
     2401 aaggatcggg cagccgaaat tagccggatc accgggtgtc agcgggcagc ggtcctcttt
     2461 atcgaccact ccgtcgccat cagaatcaga ataggggcaa ccattatttt caggtggtcc
     2521 tttcttatcc gggcacatgt catcggcatc ctttacgcca tcgccgtcgg tatcgaggta
     2581 tgggcaaccg gcatattcag ccagaccggc caggtcagga caattgtctt taatatccgg
     2641 aattccatca ccatcactat caggacaacc tgcaaatttc gcgggcccgg cgatttccgg
     2701 acattcatcc tggtcatcga ccacaccgtc gttatcaaag tcaggacatc cattggttat
     2761 tttggggccg gggactttcg gacatttgtc gtccttatca gagacaccgt cgccatcggt
     2821 atccgggcaa cccaaaaatt ccttcttgcc ggctatatca ggacaagcgt cttcactatc
     2881 agccacgccg tcgccattac gatcggggca gccatagaat tgccatggac cgggaatatc
     2941 cgggcaaata tctttctcat cagatacccc atcattgtct ttatctggtg gtgaaccctt
     3001 cttgaacagg ggaattttgg cgcccaggta aatattcgtt ccataaggat ctttgatcaa
     3061 acctgaaaac aggttggtaa cacccgcata gatgaaaccc gccctgaaac ccagcccgac
     3121 attcattttc tggaactggt tgtactggaa aggcatagat atggtgaacc atttacgctc
     3181 atagcgcggg gtaatgctgt aatttgacat ttgatgcgaa ttcggtgctc ttgaaaaccc
     3241 ctggtgcaat cctgagaatg atgtcaggtt gacgtaaaat ccctctacca cgcgtacatc
     3301 gatctgcagg ctaagtgcgg ttggaagcgt gaccatgaat ttttcatcat taccatccac
     3361 ttttcctact ccacgtcctg agagttgtct gtgatacatt gtatcgacga aattcacata
     3421 cttctgaaaa ctaaaactac cgacttctgc atttaaccag taggtgttat ctggaacgct
     3481 attatctccg ttctggtaac gatcagcata atccggcgta aaattggctg aaaagtcaaa
     3541 actgttatat tcttttttat acttaattct gccaatatcc acaatagaag ccccgacttt
     3601 cagcaaatat ttgttcttgt catttctttc cagatctttt tctccatcca tatcatagcg
     3661 aaagtccaga tattttggac ggaattcata tacaaaacca acatccagac cggcggacgg
     3721 ctttgatgta tattgaaaat tcatagaata gtcaaatatt cctgcttcgt tatcatctcc
     3781 ccagttatcc gacactccac tgtaaatatg cgaagcattg tacgacatcg gataatcccc
     3841 gtcattattt tcgccatcaa agtaatagta gaggctgtca ctttgaatat aagaagcggc
     3901 aataccctgc agcagcttga cggtgactcc tcctttaaga acatgagggc ccgtattcag
     3961 aatcgtggct gcataggtta agccgtaatc ggcaaataca tgctgaacgg cacgcatatg
     4021 atcgtcgaaa acccattgat tccagtagtt ctcattggta tagtcgcttt ggaccgttcg
     4081 aatcaggggt tcactgatgc catcgacatt ggtgatgcta cggacacgtg atgtgaagcc
     4141 aatagcctga ctatatggca gggagatcat aaagccgggc cccagaattg attgtgatga
     4201 aaacatatgt ttatccaatc cgtcgggatt atcaaggtag gtgttttcac tccaccagtt
     4261 ttcgttgttt cggtaaccgg atggattcag gatccattta gatcgtaaac gcaaagcatc
     4321 gttataaata tcactatttg caccaaaaac attgatatct accatgaagc gactgtcgga
     4381 aattgaagcc ggctgtaaaa caacctgatt gatccccatg tagttatcgc tcattgttgg
     4441 aaggaagtct tgtgctaaaa ccaggccaac cgggccaaac attaataata gcaacagcaa
     4501 cttttgtttg tagaattttg tcataatacc taagtttaaa gggttataag attgtattag
     4561 aaattgctca gattcaacct gcaaaggtca atcttatata tacggaaagc gttacacaat
     4621 taaaaataag agttatataa ttcacacatt gtcaggcaaa aagaaatttt cacctgaaac
     4681 tccgataaga ataatttgaa attggacccg aaatctatca gaaggccagg atatcattat
     4741 cgacatcggc gcggggttta cctttgaaaa cggggaattt tgcgccgata tatacattca
     4801 ggctggaagc atcattaaag atggcagaaa cc
//
LOCUS       DAOPNH010000260         4807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_20578_length_4807_cov_5.956229, whole genome shotgun sequence.
ACCESSION   DAOPNH010000260 DAOPNH010000000
VERSION     DAOPNH010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20578_length_4807_cov_5.956229"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /gene="secF"
                     /locus_tag="PKZ74_12045"
     CDS             <1..1338
                     /gene="secF"
                     /locus_tag="PKZ74_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPM93529.1"
                     /translation="AGIVLTLGMAVDANVIIYKRIKEEMRAGKGIRLAISDGYKAAYS
                     AIIDGNVTTLLTAIVLYIFGTGPVQGFATTLIIGILSSLFTAIFISRIIFTTLLDKNK
                     TISFEMPTTRGFLQNTKIDFVSLRKYAYIFSITVSVIGLISMFTRGFDQGIDFAGGRT
                     YVIRFDQPVSTIEVRNALEGQFEGNAPEVKTFGPASQVKVTTKYLIDNKGPEVDSIIQ
                     NKLFTGLKPFYTNPSFAYTDFTTDATEAGKLLGIRSSQKVDPTISDEILWRSIRAIAI
                     ALIIIFVYIALRFKKWQYGLGGLISLFHDALITLGIFSLLYSIMPFSMELDQALIAAI
                     LTIIGYSINDTVIIFDRIREYVTLHPKRDYKTNVNEAINSTLARTVNTAGTTLIVLIA
                     IFIFGGEVIRGFTFALLFGIAFGTYSSVFVATPIAYDLMMKKLKKSDKAKAIK"
     gene            1438..1794
                     /locus_tag="PKZ74_12050"
     CDS             1438..1794
                     /locus_tag="PKZ74_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPM93530.1"
                     /translation="MPQMLLFFDISGGELMVILFVAFLVFGPSKIPEVARKIGRGMNE
                     IRRASDEIKREINKESRKLEHEVNGEGSFYRDIKKSAEEIRQDFEDAAKPSDEVRKEP
                     PEENNHIGSRDSGDFR"
     gene            1856..4327
                     /locus_tag="PKZ74_12055"
     CDS             1856..4327
                     /locus_tag="PKZ74_12055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM93531.1"
                     /translation="MKNIIRYTLLLVFAILAISQAAGQKKTFIRTADEAFEDERFSVA
                     VEKYQKAYTKLKKNPVERDRISFRMAECYRMMGELKRADIQYKRLIKNGYDSKEPIIL
                     LHYADYQKADGNLTEAKEYYTKYIEKVPDDPRGKYGLEACEKIPNWQEYESKYQISEE
                     KGLNSRESDFAPTYASDNFNSLIFTSTREGSKGKGIDEWTNQGFSCLFQSRKDVKDAW
                     STPVLVDDAEEGGINTEANEGAPMMNKNYTRLYFTRCPTDEGRKNGCQIYTSNRTGRT
                     WSTPDLIPLGLDSTEAVGHPTVSDDEMILYFSSNRPGGMGGKDIWVAVRETPGGPFGR
                     PYNLGPRINTPGDELFPYLRYDTLLYFASDGHPGLGGLDIFYTFPDTARMWSTPVNIG
                     IPINSSSDDFAIIFQPKGEQGFFSSNRSGRKSREDIYSFVIPAVAFTLSGTVKDDRTL
                     QVIPGARVDIVGSDGTSMTARTSETGVYMFGKSQIMPNTTYEMTVSKDNYFNASGRET
                     TVGYEKSKDLVRNFVLEPIPEEPVVLPEILYDLAKWDLKPQYQDSLQGLITTLDENPT
                     LKIELASHTDARDTEERNDILSQRRAQSVVDYLIERGIDPDRLVAKGYGERVPRLLLK
                     DITRDGFTFKEGTILTEKYIDSLATTPEKEAAHQLNRRTEFRVISKDFVPKPKNVELG
                     KTVEILINPDDNILKYTPAPKTGNITAACIINGYTAQFTFDAKLIPQISVNEALRLLS
                     SKAIGKEDFVGNPDDILADGNIANRSKLVIKELTIANITIKNIEFLVISNLSYPIVIG
                     NSVFNAIGDYTIDNAQQQIIFRKKK"
     gene            4327..>4807
                     /locus_tag="PKZ74_12060"
     CDS             4327..>4807
                     /locus_tag="PKZ74_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HPM93532.1"
                     /translation="MADDLKYSLRGVSASKEDVHAAIKNVDRGLFPKAFCKIIPDMAG
                     NDPANCTVMHADGAGTKSSLAYIYWKETGDLSVWKGIAQDAIVMNLDDLLCVGATGPV
                     LLSSTIGRNKRLVPGEVITAIIDGTEEFLARMRSHGIEIYSTGGETADVGDLVRTIIV
                     "
BASE COUNT         1360 a         1157 c         1182 g         1108 t
ORIGIN      
        1 gccgggatcg tgttaaccct cggtatggcc gtggatgcca acgtaattat ctacaaacga
       61 atcaaggagg aaatgagggc cggcaaaggg atcagactgg caatctccga cggatataaa
      121 gccgcctatt ccgctattat cgacgggaac gtaacgactc tgcttacagc tatcgtactc
      181 tatatattcg gtacgggtcc tgttcagggt tttgccacaa cactgatcat cggtatcctc
      241 agctcgctgt tcacagctat tttcatttcc aggatcatct tcactacact tcttgataaa
      301 aacaagacca tatcatttga gatgcccacg acccggggat ttctgcaaaa cacaaagatc
      361 gactttgtca gtttacggaa atatgcttac attttttcaa tcacagtctc tgttattggc
      421 ctaatctcaa tgtttaccag gggatttgat caaggcatcg atttcgcggg cggcaggacc
      481 tatgtcattc gcttcgatca gccggtcagc accatcgagg tcaggaatgc ccttgaagga
      541 cagttcgaag gcaatgcacc cgaagttaaa acatttggac ctgcatcaca ggttaaagtg
      601 accactaaat acttgattga taataaaggg cctgaggtcg attcaattat ccagaacaaa
      661 ctctttaccg gtcttaaacc attttatacc aacccgtcgt ttgcctacac agactttacg
      721 acagacgcaa ctgaagccgg taaattgctg ggaatcagaa gctcacaaaa agttgatcca
      781 acaatatcgg atgaaatttt gtggcgttct atccgggcaa ttgctattgc tttgataatc
      841 atttttgtat atatagcgtt gcggttcaag aaatggcaat acggactcgg aggcctgatt
      901 tcattgttcc atgatgcctt gattactttg ggaatatttt cgctgcttta ttccatcatg
      961 ccgttcagca tggaattaga ccaggcgctg atcgccgcca tcctgacgat catcggttat
     1021 tccatcaacg atacggtgat catcttcgac agaatcaggg aatatgtcac cctgcatcct
     1081 aagcgggact ataaaacaaa tgtcaatgaa gctattaaca gcactctggc ccgtacggtc
     1141 aacacagccg gcactaccct gatcgtactg atcgccatct ttatttttgg cggtgaggtc
     1201 atccgcggat ttacttttgc cctgctcttt ggtattgcat tcggaactta ttcctcggta
     1261 ttcgttgcta cacccattgc atacgacctg atgatgaaga aattaaagaa aagcgacaag
     1321 gcaaaagcca tcaaatagat aaatcattac ataaaaccgg gctggttgac aaggaggtaa
     1381 aatttccgga caaccagcct tttttattac ttttgtgttc ttatcaatta cggccctatg
     1441 ccacagatgc tactgttttt tgatatcagc ggaggcgaac tcatggtcat cctgttcgta
     1501 gctttcctgg tttttgggcc cagcaagata cccgaagtcg cccggaagat cggcaggggg
     1561 atgaatgaaa tccggcgcgc atcggatgaa atcaaacggg agatcaataa agaaagccgg
     1621 aagctggagc acgaagtgaa cggcgaaggt tcgttttaca gagatataaa gaagtctgcg
     1681 gaagagatca gacaggattt tgaggatgcg gcaaaaccgt cagatgaagt caggaaagaa
     1741 ccaccggaag aaaacaatca tataggctca cgggattccg gcgacttcag gtgatcattc
     1801 aaataatttt gatgcttctc cgttaaataa aaaatgtatg aattgctgtt gtagcatgaa
     1861 aaatattatc cgatacacat tactcctggt atttgccata ctggccatca gtcaggctgc
     1921 cggacagaag aagaccttta tccgcacggc agacgaggca tttgaagatg aacggttttc
     1981 ggttgcagta gaaaaatacc agaaagccta taccaagctt aaaaaaaacc cggtggagcg
     2041 tgaccggatc tcctttcgta tggcggaatg ctaccggatg atgggagaac tcaaaagagc
     2101 cgatatccaa tacaaacggt tgataaagaa tggctatgac agcaaagaac ccatcatcct
     2161 gcttcattat gccgattacc agaaagctga cggaaacctg actgaagcaa aagagtatta
     2221 taccaaatat attgagaaag tccctgatga tccacgggga aaatatggcc tggaggcctg
     2281 cgaaaagatc ccgaactggc aggaatacga gtcgaaatac cagatttcgg aagagaaagg
     2341 attaaattcc cgggaatcag attttgcgcc gacttatgcc agcgacaatt tcaacagcct
     2401 gatctttacc tccacccgcg aaggaagcaa aggaaaaggc attgacgagt ggaccaacca
     2461 gggattcagt tgcctgttcc agtcgaggaa agatgtcaag gatgcctgga gcacaccggt
     2521 tctggtggat gatgccgaag agggcggcat aaatacggag gccaatgagg gagcgcccat
     2581 gatgaacaag aactacaccc ggctctattt tacccgctgc cccaccgacg aaggccgcaa
     2641 gaacggatgc cagatctaca catcaaaccg gaccggccgt acctggagca cgccggacct
     2701 gattccgctg gggcttgatt caacggaagc ggtcgggcat cccacggtca gcgacgacga
     2761 gatgatcctt tacttctcat cgaacaggcc gggcggaatg ggaggaaaag acatctgggt
     2821 agcagtaagg gaaacaccgg gaggcccgtt cggccgtccg tacaacctgg ggccccgcat
     2881 caataccccc ggcgatgaac tattccctta cctgagatac gatacgctgc tttattttgc
     2941 ctccgacggc caccccggac tgggaggact tgatatattc tataccttcc ccgataccgc
     3001 caggatgtgg tcaacacctg tcaatatcgg aatcccgatc aattcttcct ccgacgattt
     3061 tgctataatt tttcagccga aaggcgaaca gggatttttc tcatcaaacc ggagtggaag
     3121 aaaatccagg gaagatattt actcctttgt tattcccgca gtggctttca ctttatcggg
     3181 aactgtcaag gatgaccgga ctttgcaggt cattccgggg gcgcgtgtgg atattgtcgg
     3241 ttcggatggg acgtcgatga ccgcaagaac ctccgaaacc ggagtttata tgtttggcaa
     3301 gtcgcagatc atgccgaata ccacctatga gatgaccgta tcaaaagata actatttcaa
     3361 tgccagcggg cgggaaacaa ccgtaggtta tgagaaaagc aaagacctgg tgcggaattt
     3421 cgttctggaa ccgattcccg aagaaccggt ggtgctgccg gagattttgt atgacctggc
     3481 taaatgggat ttgaaaccgc aataccagga ttcgctgcag gggctgatca ccacactgga
     3541 tgagaatccg acactgaaga tcgagctggc ctcccatacc gatgcccgcg acaccgaaga
     3601 aagaaatgac atcctgtcgc aacgccgtgc ccagtcggtg gtcgattacc tgatcgaaag
     3661 aggtattgac cccgatcgcc tggtggcaaa aggctacggg gaaagggtcc cgaggctctt
     3721 gctgaaagac atcacccgtg acggattcac cttcaaggaa ggaaccattc tcacggaaaa
     3781 atacatcgat tcgctggcaa cgacgcccga aaaagaagca gctcaccagt taaacaggcg
     3841 gacagagttc agagtgatca gcaaggattt tgtccccaag ccgaaaaatg ttgaactggg
     3901 caaaaccgtt gagatcctga tcaatccgga cgacaatatc ttgaaataca ccccggcgcc
     3961 caaaaccggg aacattacgg cggcctgcat tatcaacggg tacacggcac aattcacatt
     4021 cgatgcgaag ctgatcccgc agatatctgt caacgaagca ctgagactgc tttcatcgaa
     4081 ggcgatcggg aaagaagact ttgtcggcaa tcctgacgac atcctggccg atggtaacat
     4141 tgccaaccgt tccaaactgg tcatcaaaga actgaccatt gccaacatta cgatcaaaaa
     4201 catagaattc ctggtgatca gcaacctgag ctacccgatt gtcattggca attcggtttt
     4261 caatgctatc ggcgattata cgatcgacaa tgcccagcag caaatcatct tccggaaaaa
     4321 gaagtaatgg cagatgatct caaatactct ctcaggggcg tatcggcatc caaagaagat
     4381 gtccatgctg caattaagaa cgtagatagg ggcctgtttc ccaaggcttt ctgcaagatc
     4441 atccccgata tggcgggcaa cgatccggcc aattgcacag tgatgcatgc cgacggggcc
     4501 gggaccaaat cgtcattggc ttacatttac tggaaagaga cgggcgatct gtctgtctgg
     4561 aaagggattg cacaggatgc gatcgtgatg aacctcgacg atctgttgtg cgtgggtgcc
     4621 accggtcctg tcctgctctc gtcaacaatc ggccggaaca aacgcctggt tccgggggaa
     4681 gtgatcacag caatcatcga tgggacggaa gaattcctgg ccaggatgcg cagccatggt
     4741 atcgaaatct actccaccgg tggtgaaacg gccgatgtag gcgacctggt gcggactatc
     4801 attgtcg
//
LOCUS       DAOPNH010000261         4784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_20722_length_4784_cov_7.112709, whole genome shotgun sequence.
ACCESSION   DAOPNH010000261 DAOPNH010000000
VERSION     DAOPNH010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20722_length_4784_cov_7.112709"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..632)
                     /locus_tag="PKZ74_12065"
     CDS             complement(78..632)
                     /locus_tag="PKZ74_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93533.1"
                     /translation="MKIFQTLINLINATAIVAFLFPLNSFSQEWERYEIDISECSVVL
                     PSEPEWYYEYSQDSSFVWSGEVQQGNMFFGVICIEFAVPFDTEEFDEYDLILIAEDYL
                     DYLKDQFSITSFTGYEHVSWIDSETGGTGISDYWTDAEGDPWAVQCWIDPYNLAVLYI
                     YSDPETDFSDYEEHFFNSFWFPEE"
     gene            complement(664..1332)
                     /locus_tag="PKZ74_12070"
     CDS             complement(664..1332)
                     /locus_tag="PKZ74_12070"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPM93534.1"
                     /translation="MAIAVLILMITAGACQKENNSVLATENEKVQANITEDSLDCYCL
                     INPSPTVSEAEIEILLFMREEEKLARDVYLAMHELYDIPIFKNIAKSEQHHMNQVLCL
                     LQFYNIPDPASPDTGIFANSDLQTLYGSLVQQGNISLIEALKAGALIEDKDIFDLESD
                     MNQTENPAILNTFSRLSCASGNHLHSFTKWLTSKGITYVPQLLSQQEYDEIITLPHQF
                     CGNR"
     gene            complement(1427..2149)
                     /locus_tag="PKZ74_12075"
     CDS             complement(1427..2149)
                     /locus_tag="PKZ74_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93535.1"
                     /translation="MNNASRIIDRIRNENIRPSPRWKFSLVNGISWLGFILAVIFGAL
                     AFSVILFAIQQVDFNILAYMPRSWLSMILSLVPLIWIVSLLLLIILAIFILRNSRKGY
                     KIPPLNLIGFCVFISMLVGTVFFITGGAGWLEQSFALRVNAYRSINEKKLDLWMMPDQ
                     GYLSGRVESVETANFELLDFNDHSWLIDYSEADIPPSVHLQAGEIIKIIGEKQSENEF
                     KALTIRPWGGGTGRQHRHHGGK"
     gene            complement(2169..2645)
                     /locus_tag="PKZ74_12080"
     CDS             complement(2169..2645)
                     /locus_tag="PKZ74_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM93536.1"
                     /translation="MFLRYEENMLRYIRRISGADKNWAQDILQDAFIKVWKNLNDYDQ
                     GLKFSSWIYRIVHNETVSSMRKGKSFGKNKEVDIDLFRNTLFEEVVDQTDQSPDPGKV
                     IQQITQLPVKYQEVMMLKFLEGKSYDEISDILRLPEGTIAIRISRAKKMLRQQHHE"
     gene            2964..3371
                     /locus_tag="PKZ74_12085"
     CDS             2964..3371
                     /locus_tag="PKZ74_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93537.1"
                     /translation="MKRIKLSKRAIILMVFSVMILTPIISFSQDIIEIQVSPNVLNLQ
                     NNGQVVTVHTEIAYWLVDAETVSMNGVAINSWKADDNGNFVAKFLMEEIVGLPGLVIG
                     DYNELTLDGTRYDGTTFTGTDEIMVINVIPKKR"
     gene            complement(3411..3950)
                     /locus_tag="PKZ74_12090"
     CDS             complement(3411..3950)
                     /locus_tag="PKZ74_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM93538.1"
                     /translation="MIAFSAVSLNAQSVTADVKNSSLKWHAKKVTGEHYGNISLKSGM
                     MEMKNNQIAGGKFVIDMNSITCTDLTDATYNAKLVNHLKSDDFFGVAKHPEAKIEIVK
                     ASAFVNGESTVDAKLTIKNITHPISFKVKQDGKSYTAEITVDRSKYDVRYGSGSFFDN
                     LGDNMIYDEFTMTVKILGS"
     gene            4225..>4784
                     /locus_tag="PKZ74_12095"
     CDS             4225..>4784
                     /locus_tag="PKZ74_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPM93539.1"
                     /translation="MGGFFGIISRADCVSDLFYGTDYNSHLGTKRGGMAVLSSKEFKR
                     AIHNIENSYFRTKFEPDLSKMYGNSGIGVISDGGTNLSELIAMLIDEESSFERGIENV
                     FDKVKGSCSILLLTREGLYAARDKLGRTPVVIGRKEGAFAASSESSAFFNLGYKTEKF
                     LGPGEIVFMTAEGYEQRRKPNDKMQI"
BASE COUNT         1383 a         1049 c          961 g         1391 t
ORIGIN      
        1 agtaattttt ttcatcgggt taatgaattt aaaacgagtt caaaggtact aaaaaaaggg
       61 ttggaaaccc tttcttttta ctcctccgga aaccagaagc tgttgaagaa gtgttcctca
      121 taatccgaaa aatcggtttc gggatcgctg taaatataga gcaccgcaag gttgtatgga
      181 tcgatccagc attgaacggc ccaggggtcg ccctctgcgt cggtccagta gtcggatatt
      241 ccggttcccc cggtttctga atctatccat gagacatgtt cataaccggt aaaagaagtt
      301 atactgaatt gatctttaag ataatccagg taatcctcgg cgatcaggat gagatcatat
      361 tcatcaaatt cctccgtatc aaaaggcacg gcgaactcga tacaaatcac accgaaaaac
      421 atattcccct gctgcacctc accgctccag acaaagctgc tgtcctgtga atattcgtaa
      481 taccactccg gctctgaagg caatacaacc gagcattcgc tgatatcgat ctcataccgt
      541 tcccattcct gggagaacga attcagcgga aataaaaaag ccactatagc agtggcattt
      601 ataagattaa taagggtttg aaaaatcttc atggtgcgat ttttcagtaa aggtataaaa
      661 actttaccgg ttgccacaga actggtgagg caaagtaatt atttcatcgt actcctgctg
      721 gcttaagagt tgcggcacat acgtaatccc tttgctggta agccattttg taaatgaatg
      781 cagatgattc cctgaagcac acgacaacct ggaaaatgta tttagaatcg ccggattttc
      841 agtttggttc atgtctgatt ccaaatcaaa gatatcttta tcctcaatca acgcaccggc
      901 tttaagtgcc tcaatgagag aaatattacc ctgttgcaca aggctgccat atagcgtctg
      961 caaatcagaa ttcgcaaata ttcctgtatc gggagatgcc ggatcgggga tattgtaaaa
     1021 ttgcagcagg cataaaacct gattcatatg atgttgctcg ctcttggcta tatttttgaa
     1081 gataggaatg tcatacaact catgcatagc caggtaaaca tcacgagcca gtttttcttc
     1141 ctcccgcatg aacagcagta tctcaatttc agcttctgaa acagtcgggg aaggatttat
     1201 aaggcaataa caatcgagtg aatcttcggt gatattagcc tgcacttttt cattctcagt
     1261 cgccaaaacc gaattattct ctttttggca ggctcctgcg gtaatcatta gtattaagac
     1321 ggcaatagcc accttaaccg ttaaattgaa caatctactc atataataaa gttttgtagt
     1381 taataaatcc gttatgttca ataatacgta acatcgaaat tatctttcat tttcctccat
     1441 gatgcctatg ttgtcggcct gttccaccac cccatggcct gattgttaac gctttaaatt
     1501 cattctcaga ctgcttttca ccgattattt taatgatttc gcccgcctgc agatggactg
     1561 acggaggaat atcagcctca gagtaatcaa ttagccagga atggtcatta aaatcaagca
     1621 gctcaaaatt agctgtttcc acagattcca ctctgccgct caggtatccc tggtccggca
     1681 tcatccaaag atccagtttc ttttcattaa tgctcctgta ggcgttgacc cgcaatgcaa
     1741 acgactgctc aagccatcct gccccaccgg taatgaaaaa tacagtgccg acaagcatgc
     1801 ttataaacac acaaaacccg atgaggttga gtggcggaat tttatacccc ttcctggaat
     1861 tcctcaaaat aaatattgca agaataatca gcagaagtaa ggaaacaatc catatcagcg
     1921 ggaccagact aagtatcata gataaccatg atcgcggcat gtacgcgaga atattgaaat
     1981 cgacctgctg aatagcaaat aggatcactg aaaatgccag tgctccgaaa atcactgcaa
     2041 ggatgaatcc cagccaggaa attccattta ctaaactgaa tttccaacga ggactgggtc
     2101 ttatattttc attcctgatc ctgtctataa tcctggaagc gttattcata tttctatttt
     2161 taacaatctc attcatgatg ctgttgtctc aacatttttt tcgcccggct tatcctgatg
     2221 gctatagttc cttccggcaa tcgcaggata tctgaaattt cgtcataact tttaccttca
     2281 aggaatttca acatcatcac ttcctgatat ttgacaggaa gttgcgttat ttgttgaata
     2341 acttttcccg gatccgggct ttggtcggtc tgatcaacaa cttcttcaaa aagggtatta
     2401 cggaataaat cgatatcgac ctctttattt ttgccaaaag attttccttt cctcattgag
     2461 gaaacggttt cattatgaac gatccggtat atccagcttg aaaatttcag tccctgatca
     2521 tagtcattga ggtttttcca aacttttatg aaagcatcct gcaggatatc ctgcgcccaa
     2581 tttttatctg ctcccgaaat ccgccggata tacctgagca tattctcttc atatcgcagg
     2641 aacaaacaag agaaataatc aagattctct atcgataatc ttgccaggtc cttatctgtt
     2701 aatcctttac aactttccgg ggttataatc atctcgaccg gccattaaac agatgaatta
     2761 gctttcaata aacatcaaac cttttatacg ctccggcaaa ctcaatcttt caaaataata
     2821 ttcttaaatc cagattacat tgtataaaaa gatagattaa tagctatatt tagagtactt
     2881 tttgaaagat ttactaatgc tacgcgtact aaaggtactt ccgtcatcct gtattttatt
     2941 caaaacaatt aaaaaaattc gacatgaaaa gaattaagtt aagcaaaagg gccattatcc
     3001 tgatggtttt ttctgtgatg atcttaaccc cgatcatcag tttctcacag gatattattg
     3061 aaattcaggt ttctcccaat gtactcaact tgcagaacaa cgggcaggta gtcacggttc
     3121 atacggaaat agcatattgg ctggtggatg ccgaaacagt gtcgatgaat ggagtcgcta
     3181 tcaatagctg gaaagccgat gataacggga acttcgtggc gaaattttta atggaggaga
     3241 ttgttggatt gccgggatta gttatcggtg actataacga gctgaccctg gatggtaccc
     3301 ggtatgacgg tacaactttt acgggtaccg atgaaataat ggtgataaat gtgattccga
     3361 agaaacgata agcaaggatg ctcaggtttc tgagcatcct attttgattt ttaactaccc
     3421 agaattttca cagtcatcgt aaattcatca tagatcatgt tgtcgcccag gttgtcgaaa
     3481 aatgaaccgg aaccgtagcg gacatcatac tttgaacgat cgacagtgat ctcagccgtg
     3541 taggattttc cgtcctgctt cactttaaag gagatgggat gggtgatgtt cttgatcgtc
     3601 agctttgcat ccactgttga ttcgccattg acaaacgccg atgccttcac aatctcgatc
     3661 ttcgcttccg gatgttttgc cacaccgaag aaatcatccg attttaagtg gttgaccagc
     3721 ttggcgttat atgtcgcatc ggtcaggtcg gtgcaggtga tgctgttcat atcgataacg
     3781 aacttcccgc cggcgatctg gttgtttttc atctccatca tgcccgattt caggctgatg
     3841 ttgccgtaat gttcaccggt aacttttttg gcatgccact tcagtgagga gttcttcaca
     3901 tcggctgtta cactctgcgc attcagcgac actgccgaaa acgctatcat aatcaaggaa
     3961 ataataagaa ttctcatttt catactgttt aaagatttat aaattaattt acacatgata
     4021 tctaataact tatatgtatc tatattgata cccgagtcga tgaaacaagc ttccggaaga
     4081 tttgtttaag catctgagaa aaaaatgtca caatcctgaa acttttgttc aatattactt
     4141 ttcaacaggt tggtcatcca ttacattttt cttatagttt catcggatcg caaaaactca
     4201 tttaaccaac caaaaaataa tcgtttgggc ggattcttcg gaatcatttc ccgggcagac
     4261 tgcgtctccg atctcttcta cggcaccgac tacaattccc atctcggcac aaagcggggc
     4321 ggaatggccg tgctcagttc caaagagttc aaacgggcga tacacaatat cgaaaactcc
     4381 tatttccgga ccaagttcga acccgatctc agtaaaatgt atggcaacag tgggattggg
     4441 gtgatcagcg acggcgggac caacctgtcg gaactgatcg ccatgctgat cgatgaagaa
     4501 tcatcatttg agaggggcat tgaaaatgta tttgataaag ttaaaggctc ctgttccatt
     4561 cttttactta cacgggaggg tttgtatgct gcccgcgaca agctgggccg gacgcccgtg
     4621 gtgatcggcc ggaaggaagg cgcttttgcg gccagtagcg aatcgagtgc tttttttaac
     4681 ctggggtaca agacagaaaa gtttctcggg cccggggaga tcgttttcat gaccgccgaa
     4741 ggctatgagc agcggcgcaa acccaacgac aagatgcaga tttg
//
LOCUS       DAOPNH010000262         4730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21046_length_4730_cov_6.461390, whole genome shotgun sequence.
ACCESSION   DAOPNH010000262 DAOPNH010000000
VERSION     DAOPNH010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21046_length_4730_cov_6.461390"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKZ74_12100"
     CDS             <1..306
                     /locus_tag="PKZ74_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93540.1"
                     /translation="SGLWKVYYPNGQVKQSGVFKNGISEGPWQFFHDNGAVKMKGTYV
                     NNKENGNWVHYYNTGKIKLDLYYKDGKLEGTQSGFDPEGNLAEKAIYSEGLLIEKIK"
     gene            309..884
                     /locus_tag="PKZ74_12105"
     CDS             309..884
                     /locus_tag="PKZ74_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93541.1"
                     /translation="MKPGILIIILFLLLGPVKNRINGQDIPPPNPGLVINIVTGYSIQ
                     FVFDEIDEYINGIQNAGQSTFIRIGAIYDWKLQFKADQDMFYGTFDPAHQMELNNVGV
                     TVNSIGTNLDDGTHILNYAKAAPLALEYTDVTLLTKGTLSNKGYGIENTFTLDWEMGT
                     RNGNMNQTRMLDQLIPADSYTLNIILTLSVY"
     gene            1112..1732
                     /locus_tag="PKZ74_12110"
     CDS             1112..1732
                     /locus_tag="PKZ74_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93542.1"
                     /translation="MKKIVLLFGLLFALQAAIMAQLTDQEVVVFNAHLNETFNLNVDG
                     VTQEITFAVAADYNNGVTEAGGIVPGFTLISVEATGDWELEISAPDFTPGGGPNPGTG
                     AIPINNLGVWCQATGTHQFGTEVTCAYQNAAAAMGLTNADQLLIGLGTTNAGDISDNA
                     FTLHWLMGTMQGSMNTESMFNQLALGTLFSTGDFTTTATLTLTEIP"
     gene            1882..2502
                     /locus_tag="PKZ74_12115"
     CDS             1882..2502
                     /locus_tag="PKZ74_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93543.1"
                     /translation="MKRIILLLICFTAIQLNSVTAQLTDDEEVVFNAILGGAFNLVVQ
                     DGDVQTATFGAADEYNLGISETMGNPGIDPGFTTVAMEATGNWYLKISAPDFTPIVGT
                     GVIPINNLGVWCEATGVHQFGTEVACAYQSADAALGLSNTDITLIDLLTDNSGDQTDN
                     LFVLHWLFGTMQGTMNPASMFSQLSDGIFSQGTYTSTVVLTMTEIP"
     gene            2607..3236
                     /locus_tag="PKZ74_12120"
     CDS             2607..3236
                     /locus_tag="PKZ74_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93544.1"
                     /translation="MDKLFKIRLLTTLLTGFSAIYAFSQETPMLISTEAKLDNVLLLK
                     MTPEKYVEFTIKKINENLYQITDQPDDVMFSVESTGNWNLSISAEDPYFTGVSDSSHK
                     IPVDFIGLFVENIGSNWDNGLFSNMANATKDTVVSLSPDKTSLLEAGVRGNVGGSLQN
                     AFILRWKFFYEDAALKTRKFTDYIIEDDTYKVGYYITLSESTANSKIDQ"
     gene            3455..>4730
                     /locus_tag="PKZ74_12125"
     CDS             3455..>4730
                     /locus_tag="PKZ74_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93545.1"
                     /translation="MIRIRNLSDSAIRIKPVLMLPDGWLSFTTPVNDTLIGANDSISI
                     PIRFQLPEKVSSEISHDIAFRAYSMQNKLLSETHCTVKPEAFHKWDVTLPDNRVFFYP
                     RNNNSDFTIFIENKGNIEEQIDLNIITDRKVELVSTGAWQPGQPINIKPFQDTTLKLN
                     AKYINDDQRVFDLSKIQVIAEAGEAKFNQSFMIEKYNDTYAPLIFDRTLPHQVELGIR
                     TFTGNQEFLPFIKARGIATIKSTSSFTYNFNYYSVTGAENIISNSYYNFLYKWKSLKV
                     GLGAFSSQLGRNLYTRNGIMASNILKLSDHLNMEVFLSQSFLVPKTSIASGFTYEKKK
                     LELRGSLAYDMDKDKGINTGSAMVQSGLIKIFKDHDFTFSLYGYHETHDVTNDYTLAG
                     VAWDINYFAKIGDMVSVQVTNNYGSPNMPGPQM"
BASE COUNT         1347 a         1136 c         1018 g         1229 t
ORIGIN      
        1 tcaggtctgt ggaaggttta ttatccgaac ggccaggtga aacaaagcgg tgtatttaaa
       61 aacgggattt cggaagggcc ctggcagttc ttccatgata atggcgcagt aaaaatgaag
      121 ggtacctatg ttaataacaa ggaaaacgga aactgggttc attattataa taccggaaag
      181 ataaagcttg atctgtatta caaagacggc aagctggaag gtacccaatc gggttttgac
      241 cctgaaggca acctggcaga gaaagccatc tattcggaag gattgctcat tgagaaaatc
      301 aaatgatgat gaaacccggc atcctcatca tcatcctgtt cttgcttctt ggaccggtca
      361 aaaaccggat caatggccag gatatccctc cgcccaaccc gggtcttgtc atcaatatcg
      421 taaccgggta cagcatccag tttgtttttg atgagatcga tgaatacatc aacgggattc
      481 agaatgccgg ccagtcgaca tttatccgta tcggtgcaat atacgactgg aaactccagt
      541 ttaaagccga tcaggatatg ttttacggca cctttgaccc tgcccaccag atggagctta
      601 acaatgtcgg tgttacggtc aactcgatcg gtaccaacct ggatgatggt acccatatcc
      661 tgaattatgc aaaggccgca cctttggccc tggaatatac cgatgtgact cttctgacaa
      721 aaggtacctt atctaataaa gggtatggca ttgaaaacac ctttacgctc gactgggaga
      781 tgggcacccg gaacggaaat atgaaccaga ctcgcatgct cgaccagctt attccggcag
      841 attcatatac cttaaacatt attctcaccc tttcggtata ttaagttttc gttttttttt
      901 cgagatttct gcgatttttt gaaacctatc catttttccc tcgttaaatt ttttacagaa
      961 gagttcatta tttatacttg atttaaatta ggagtgtccg gttaaatttt ttcaggtttg
     1021 gaataacctt ttatcaacaa gctgattaat attaacctga ccacctccca agaaattgtt
     1081 aataaatcat cttaatctta aaattaaaat tatgaaaaag attgttcttc ttttcggatt
     1141 gctgttcgca cttcaggcag ccattatggc tcaattgacc gaccaggagg tggttgtgtt
     1201 caatgctcat ctcaatgaaa ctttcaacct gaacgttgac ggtgttacac aagaaattac
     1261 attcgccgtt gcggccgatt acaacaacgg tgtcacggaa gccggcggta ttgttccggg
     1321 atttaccctg atcagcgtcg aagcaaccgg cgactgggaa ctggagatca gcgcccccga
     1381 ttttacaccg ggtggtggtc ctaaccccgg aaccggtgct atcccgatca acaacctggg
     1441 tgtatggtgc caggctacag gtactcacca attcggcacc gaagtgacct gcgcatacca
     1501 gaatgctgct gcagccatgg gtcttaccaa tgccgaccaa ttgctaatcg gactgggaac
     1561 aaccaacgcc ggggacatct ccgacaacgc ctttacgctg cactggctga tgggtaccat
     1621 gcagggatcc atgaacactg aaagtatgtt caaccagttg gctctcggta ccttgttctc
     1681 gaccggtgac ttcacgacca cagccactct gaccctgacc gaaattccgt aagaatttta
     1741 acgcatatga aagaagggga catgggacca attacgccat gtttcctttt ttcatgatga
     1801 atgtttccca cgcagacatt accagggaag tgtttttcac acccgattat cacccttcgt
     1861 tccaacatca gatttaaaat tatgaaaaga ataattctgt tacttatctg tttcactgcc
     1921 atccaattaa acagtgtaac agcacaactg accgacgacg aggaagtagt tttcaatgcc
     1981 attttgggag gagctttcaa cctggtcgtg caggatggag atgtccagac agctacattt
     2041 ggcgctgccg atgaatacaa cctgggtatt tccgaaacaa tggggaatcc gggtatcgat
     2101 cccggtttta caaccgtcgc gatggaagcc accggaaact ggtacctgaa aatcagcgca
     2161 cctgatttta cccccatagt gggaacaggc gtcattccga tcaataacct gggagtctgg
     2221 tgtgaggcta ccggcgttca ccaattcgga accgaggtgg cctgcgctta ccaaagtgcc
     2281 gatgccgccc ttgggctttc caatactgat atcaccctga tcgacctttt aacggacaat
     2341 tccggggatc agaccgacaa cctgttcgtt ttgcactggc tgttcggtac catgcaaggg
     2401 acaatgaacc ccgccagcat gttttcacaa ctgtctgacg gtattttcag ccagggaact
     2461 tatacttcga ctgttgtact gacaatgacc gaaataccct aacgatcttt tcttcataac
     2521 ttaaaaagga aataaactat taaatgttaa catcttaata gttctatttc ctttttttat
     2581 tgaggattac gattatcttt ggaagaatgg acaaactttt taaaataagg ctcttaacta
     2641 ctctgctgac agggttttcc gctatttatg cgttctccca ggagaccccg atgctgatca
     2701 gtaccgaggc taaactcgat aatgtgttgt tattgaagat gacgccggag aaatatgtcg
     2761 aatttaccat taaaaagatc aatgaaaatc tttaccagat aacggatcag ccggacgatg
     2821 tgatgttcag cgtcgaatcg accggcaact ggaacctttc catttctgca gaggatcctt
     2881 attttaccgg agttagcgat tcttcacaca agattcccgt cgatttcatc gggctgtttg
     2941 ttgaaaacat aggctctaac tgggataacg gtcttttctc caacatggct aatgcaacca
     3001 aagacacggt ggtcagcctg tcgcccgata aaaccagttt gctcgaggca ggcgtcaggg
     3061 gaaatgtcgg cggctcgctt caaaacgcat tcatcctccg gtggaagttc ttttatgaag
     3121 acgcggccct gaagaccagg aaattcaccg attacatcat tgaagacgac acatacaagg
     3181 tggggtatta tatcacttta tcggaaagta ccgcaaacag taagattgat caataaaatc
     3241 cctgcatagg ggccggcaga gaccggtaat ttatccgcta taaaaactga aacttgactt
     3301 taagatactc attttttcag ggctcctgtt cttatcgggg aaaagcctga tctcacaagc
     3361 ccggcaacct gacggaggac aggctggcgc catcctttcc gcttcattca tcaaacccaa
     3421 tgtcggcctg aaaagtggcg ctcttagttt taatgtgatc cgaatcagaa acctttccga
     3481 ttctgctatc cgaattaaac cggtactcat gctccccgac ggctggctct cctttaccac
     3541 acccgttaat gatacgctga tcggagccaa cgactccatc tccatcccga tccgttttca
     3601 acttcccgaa aaagtcagtt ccgaaataag ccacgacatc gctttccggg cctactccat
     3661 gcaaaacaaa ctcctctcgg aaacacattg tactgtcaaa ccggaggctt ttcacaaatg
     3721 ggatgttaca cttccggata acagggtatt tttttatccc cgtaacaaca attctgattt
     3781 caccattttc attgaaaata agggcaatat tgaagagcag attgatctta atatcattac
     3841 cgaccgaaaa gtggaactgg tcagtaccgg agcatggcaa cccggccaac ccataaatat
     3901 aaaaccgttc caggatacga ccctgaaact gaacgccaaa tatatcaatg atgaccagcg
     3961 ggtatttgac ctgagcaaga tacaggtaat cgccgaagcg ggcgaggcta aattcaacca
     4021 gtcgttcatg atcgaaaaat acaacgacac gtatgcaccg ctgatcttcg acaggactct
     4081 cccccatcag gtagagctcg ggatcagaac attcacaggc aaccaggaat tccttccgtt
     4141 catcaaagca agaggaatag cgacaataaa gagcaccagc tcatttacct ataatttcaa
     4201 ttactattcc gtaacagggg ctgagaacat catcagcaac agttattata atttcctgta
     4261 taaatggaaa tcactaaaag tggggctggg cgctttcagc tctcaactcg gaagaaacct
     4321 ctatacccgg aatgggataa tggcttccaa catcctgaaa ttatcagatc atctcaacat
     4381 ggaagtattt ctcagtcaaa gttttctggt tccgaaaacg agcattgcca gcggattcac
     4441 ctatgagaaa aagaaactgg aactgcgcgg ttcgctggct tatgatatgg ataaagacaa
     4501 agggatcaac acaggttctg ccatggtcca gtcgggcctc atcaaaattt tcaaggacca
     4561 tgatttcact ttcagcctct atggatacca tgaaacccac gacgtgacca acgattacac
     4621 gctggcggga gtagcctggg atatcaacta ttttgcaaag ataggcgaca tggtatcggt
     4681 ccaggtcacc aacaattacg gttcacccaa catgccgggc cctcagatgg
//
LOCUS       DAOPNH010000263         4718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21120_length_4718_cov_5.965044, whole genome shotgun sequence.
ACCESSION   DAOPNH010000263 DAOPNH010000000
VERSION     DAOPNH010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21120_length_4718_cov_5.965044"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PKZ74_12130"
     CDS             complement(<1..1515)
                     /locus_tag="PKZ74_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93546.1"
                     /translation="MKFPILPILLLTLLGWMVAPPAAAQKIVYSEKDNDDTRNLNFEI
                     IGKISGNFLIYKNTRARSWISLLDNDMLPVGESKLEFLPNDDRMINVDFFPYPDHAWM
                     IYQYQRRSVVYCMAARIDGKGMKTGELIELDTTHLGFAADNKIYSVISSEDKKKIGVF
                     KINNRNRQLYLMTTLLFNDKLELLKKSKLQIPMEERNENLSDFSLDNEGDIVFSKFLR
                     QGNDNISTASFIIKKAQEDTLIQKHLNIEKTWLDEIHIKVDNFNKRYFLTSYYYKERR
                     GNIDGYYFYIWDKVNGQPLVENIHIFSDELRNEARGDASARTAFNDYFIRQIITRRDG
                     GFLIGSEAYYTTSRFNSWNRWDYLYGGSPYYFPGASNYYSPYYDRFRWGRGGNSNQSV
                     RHHADNITVMSFNSQGQLEWNTVMGKTQFDDENGDMISYQLMNTGDQIHFLFNQMERR
                     NNLLNDYGVTPSGGMTRNPTLKNLDKGYEFLPKYGKQVSAKQMLIPCLYRNYICF"
     gene            1809..3785
                     /locus_tag="PKZ74_12135"
     CDS             1809..3785
                     /locus_tag="PKZ74_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPM93547.1"
                     /translation="MKQPANKVTAAGLLIALGIIYGDIGTSPLYVFNAIIGDRLVNEE
                     LILGTLSCIIWTLTLQTTMKYVVLVLRADNRGEGGTFALYALVRRRKKWLVIPAMIGG
                     ASLLADGIITPPISITSAVEGLKQLPQLGIEDGSNTVVIIVLSILTLFFFMQQFGTAS
                     IGKLFGPIMMIWFTMLLVLGVFHVFDDLAIFKALSPYYAIHFLQTYKEGFWLLGAVFL
                     CTTGAEALYSDLGHCGRSNIRMSWIYVKACLLIHYFGQGAYLLSHRAGLTITPEVKSA
                     LSINAFYNLMPQWFIIPGVIIATVAAIIASQAMISGAFTLISEAMRLNLWPKLRIRYP
                     SEAKGQLFIPTINFLMFLGCAGVVLFFRMSSNMEAAYGLAIIVTMLATTMLYANYLVL
                     QRAKPIWIYIFLGSYLVVEIGYLVALMVKFTHGGYITLIIGLVMFLVMYVWYRARKIK
                     NRYVEFVRLEHYIPKIQELSNDKSVPKYATHLVYMTSANNPKEIEHKIIYSILNRKPK
                     RADIYWFVHIDTVDDPYTCEYVVEHIIPNDIIRVDFRLGFRMEPRINLMFRKVVEDLV
                     ANKEVNIISRYESLASSGTVGDFQFMVMEKYLSQDNELPFFERVVMKFHFWLKDHSLS
                     EEKGFGLDLANVTVEKFPLIVAPVTNLKLKRVEE"
     gene            complement(4010..>4718)
                     /locus_tag="PKZ74_12140"
     CDS             complement(4010..>4718)
                     /locus_tag="PKZ74_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM93548.1"
                     /translation="LKNITPFKELDVAKTNFIATVSHELKTPLASSDFSLKLLEDERI
                     GKLTHEQKELVQNLKEDNKRLLRILSELLDMSQVESGKIQLTLQETSAISLVEKAINA
                     VSSVAREKKIALRMKKDEVAGEVYADAEKCAWVLNNFLTNAIRYSTEGQVIDVSVRPS
                     GKQLEFSVRDFGKGIDPQYKEKIFDRYFRVPGSKEGTGLGLAICKEFIEAQGGKIGVE
                     STPGEGSLFYFTLPLKE"
BASE COUNT         1162 a         1157 c         1063 g         1336 t
ORIGIN      
        1 aaaacaaata tagttacgat ataaacaagg aatgagcatt tgttttgcac ttacctgttt
       61 cccgtacttg ggtaaaaatt catagccctt atccaggttt ttcagcgtag gattccgtgt
      121 cataccaccg gagggtgtca ccccataatc attgagcagg ttattccttc tttccatctg
      181 gttaaaaaga aaatggatct gatcaccggt attcatcagc tggtaggaaa tcatatcccc
      241 gttttcgtca tcaaactggg ttttacccat caccgtattc cattccagct gtccctgact
      301 gttaaaggac atgacggtga tattatccgc atggtgccgg acggactgat tactgttacc
      361 tccccggccc catctgaacc ggtcatagta gggggaataa taattacttg cacccggaaa
      421 ataataaggg gagccgccat acaagtaatc ccagcggttc cagctattaa accgggaagt
      481 ggtataatat gcctcactcc cgatcaggaa tccgccgtcg cggcgggtaa taatctggcg
      541 gatgaaataa tcattgaaag cggtgcgggc actggcatcc ccgcgggctt cattccgcaa
      601 ctcatcggaa aaaatatgga tgttctccac caggggctga ccgtttacct tatcccagat
      661 atagaaataa tatccgtcaa tatttcctct tctctccttg taataataag aggtcaggaa
      721 atagcgctta ttgaaattat ccaccttgat atgaatttca tccagccagg ttttttcgat
      781 attcaggtgc ttttgaatca gggtgtcttc ctgggctttt ttaatgataa aggaggccgt
      841 gctgatattg tcattgccct gacggaggaa tttggagaag acgatatcgc cctcattgtc
      901 caggctgaaa tcactcaggt tttcattgcg ttcctccatc ggaatctgga gtttactttt
      961 tttcaggagt tccagtttat cattgaacag cagggtggtc atcaggtata actgccggtt
     1021 acggttgtta attttgaaga ccccgatttt cttcttgtct tcgctgctga tcactgaata
     1081 gatcttatta tccgcggcaa atccaagatg ggtggtatcc agttcgatca gttcgccggt
     1141 tttcattccc ttgccgtcga tccgggcggc catgcagtat accacactgc ggcgctggta
     1201 ttggtaaatc atccaggcat gatcgggata gggaaaaaaa tccacattga tcatccggtc
     1261 atcgttgggg aggaactcca gtttactttc ccctaccggc aacatatcgt tatccagcaa
     1321 actgatccag ctgcgggcgc gggtattttt gtaaatcagg aaattaccgc tgattttacc
     1381 gattatttcg aagttcaggt tccgcgtatc atcgttatct ttttctgaat acacgatttt
     1441 ctgggcggca gccggcggtg caaccatcca tccaaggagg gtcagcagca atatgggcag
     1501 aatggggaat ttcataaagt taaaatctta ttaaaggtaa gggttgaacc gaaacagtcc
     1561 ttcccttttc catgatgcaa tttggtacca atttacattc cgggtatccg acaacggtaa
     1621 aatcccgccc gaacccccgt tagtaattaa acaaaagact aagatgaaag ttcttcaggt
     1681 tgttcgttgt atagaatttt tgcctatacc tttgcctttc tgccgttaca gaagcaaaat
     1741 ctgtacccgc cttcccggta actggaagac cgtcgggccg ccagaaaaca attaacaaat
     1801 accaaaaagt gaaacaacct gctaacaagg tcaccgcagc cggcttacta attgcgttgg
     1861 gcattattta cggcgatatc ggtacttccc ccttgtatgt ttttaacgcg atcatcggcg
     1921 accggctggt caatgaagaa ttgattctgg gcaccctttc ctgtatcatc tggaccctga
     1981 ccctccagac caccatgaaa tatgtggtac tggtactccg ggccgacaac cgcggggagg
     2041 ggggcacttt tgccctgtat gccctggttc gccggcgtaa aaaatggctg gttattccgg
     2101 ccatgatcgg gggggcttcc ctgctggctg acggcatcat taccccacct atctcaatca
     2161 cttcggcggt ggagggacta aaacagttgc cccaactcgg tatagaagat ggcagcaaca
     2221 cggtggtgat catcgtactc tccatcctga cccttttctt cttcatgcag caattcggta
     2281 ctgcgtccat cggtaaattg ttcggaccga tcatgatgat ctggtttacc atgctgctgg
     2341 tactgggtgt gtttcacgtc tttgatgatt tagccatttt caaagccctc agtccgtatt
     2401 atgccattca ttttttgcag acctataagg aaggtttctg gttactcggt gcggtatttc
     2461 tctgtaccac cggggctgag gccctgtaca gcgacctcgg acactgcggt cgcagcaata
     2521 tccggatgtc ctggatttat gtaaaagcct gcctgctgat ccattatttc ggtcagggcg
     2581 cttacctgct ttcgcaccgg gccggactca ccattacccc tgaagtaaag agtgccctga
     2641 gtattaatgc cttttataac ctgatgcccc agtggtttat catccccggg gtgattattg
     2701 ctaccgtggc agccattatc gccagccagg ccatgatctc cggcgccttt accctgatca
     2761 gtgaagccat gcggctcaac ctctggccca aactccggat ccggtatcca tcggaagcca
     2821 agggacagct atttattccc acgattaact tcctgatgtt cctgggttgc gcaggcgtgg
     2881 tacttttttt ccgtatgtcc tccaatatgg aagcggctta tggtctcgct atcattgtga
     2941 ccatgctggc caccaccatg ctttatgcca attacctggt actgcaaaga gccaaaccca
     3001 tttggatcta tatttttctc ggatcctatc tcgtggttga aatcggctac ctcgtggccc
     3061 tgatggtaaa gttcacccat ggtggttata tcaccctgat catcggcctg gtgatgttcc
     3121 tcgtgatgta tgtctggtac cgggcccgca agatcaaaaa ccggtacgtg gaatttgtcc
     3181 ggctggaaca ttatatcccc aagatccagg aactcagcaa tgataaatcg gttcccaaat
     3241 atgccacgca ccttgtttat atgaccagtg ccaataaccc gaaggaaatt gagcataaga
     3301 ttatttactc gatcctgaac cgcaagccca agcgggccga tatttactgg tttgtacata
     3361 ttgatacggt ggatgatcct tatacctgcg aatacgttgt ggaacatatc attcccaatg
     3421 atatcatccg ggttgatttc cgtctcggtt tccggatgga gccccggatc aacctgatgt
     3481 tccgcaaagt ggtcgaagac ctggtggcca ataaggaagt gaatattatc agtcgttatg
     3541 aaagtctggc cagcagcggt accgtaggcg acttccagtt tatggttatg gagaaatacc
     3601 tgagccagga caacgaactg cctttcttcg aacgcgtggt aatgaaattc catttctggc
     3661 tgaaagatca tagcctttct gaagaaaaag gattcggtct tgaccttgct aatgtaaccg
     3721 tggaaaaatt cccgctgatc gtggcacccg tgacgaatct gaaactgaag agagtggagg
     3781 aataagtacg aagtacgaag ttagaagtac gaggagctgt tcgtaaaccg agtatcattt
     3841 gtcatttaag aaacatgatt cttagtacgg gctgttgact cccgactact aacttcggac
     3901 tcccgactga tttcagaagg ggctgtttcc caacaactaa cagcgaacaa cgaacacctt
     3961 tcagaatagg ctgtttcctg acatccgaca tctgacaacc gacaactttt tattctttca
     4021 gtggcaacgt gaaataaaac aagctccctt cgccgggagt ggattccact cctatttttc
     4081 caccttgtgc ctcgataaat tctttgcaga tcgcgagtcc aagtccggtt ccttcttttg
     4141 aacctgggac ccggaaataa cggtcaaata ttttttcctt atactgggga tcaatgcctt
     4201 ttccaaaatc ccggacactg aactcgagtt gttttccgga cggccggacg cttacatcaa
     4261 tcacttgtcc ctcggttgaa taccggatcg cattggtcag gaaattattc aggacccagg
     4321 cacatttttc agcatcggca tagacctcac ccgctacctc atccttcttc atgcgcagtg
     4381 ctattttttt ctccctggca acggatgaga ccgcattaat tgctttttca accaacgaaa
     4441 tggcggatgt ttcctgtagt gtcagttgaa tttttccgct ttctacctgt gacatatcca
     4501 gcaattcact cagtatccgc aacagtcgct tattatcttc cttcaggttt tgtaccagtt
     4561 ccttttgttc atgggtcaac ttaccaatgc gttcatcttc caggagtttc aggctgaaat
     4621 cagaagaagc caggggcgtt ttgagttcat gagagacggt agcgataaaa ttcgttttag
     4681 caacatccag ctctttaaag ggggtaatat ttttcagc
//
LOCUS       DAOPNH010000264         4673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21429_length_4673_cov_7.532915, whole genome shotgun sequence.
ACCESSION   DAOPNH010000264 DAOPNH010000000
VERSION     DAOPNH010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21429_length_4673_cov_7.532915"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKZ74_12145"
     CDS             complement(<1..639)
                     /locus_tag="PKZ74_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPM93549.1"
                     /translation="MAKEIIFDLAAREKLKKGVDELSNAVKVTLGPKGRNVIIQKSFG
                     SPTITKDGVTVAKEVELKDNAENMGAQMVKEVASKTSDLAGDGTTTATVLAQAIFTTG
                     LKNVTAGANPMEIKRGIDLAVEKVIKSLKEQTKEIGDSFEKIEQVATISANNDFAIGK
                     LIAEAMAKVKKEGVITIEEAKSMETYVDVVEGMQFDRGYISAYFVTDTEKMEA"
     gene            complement(678..947)
                     /locus_tag="PKZ74_12150"
     CDS             complement(678..947)
                     /locus_tag="PKZ74_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPM93550.1"
                     /translation="MSIKPLADRVIVEPAIAEEKTAGGIIIPDTAKEKPQRGTIVAVG
                     PGKKDEPLTVKVGDEVLYAKYAGTEFSYEGKNFLIMRESDIVAVI"
     gene            complement(1087..1413)
                     /gene="secG"
                     /locus_tag="PKZ74_12155"
     CDS             complement(1087..1413)
                     /gene="secG"
                     /locus_tag="PKZ74_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPM93551.1"
                     /translation="MGLYILVSVLVLVACILLILVVLVQNSKGGGLASNFGSTGQIMG
                     VRKTADFLEKTTWSLAIALLALSLISILVIPRNATTGSTQDTELREKIENMQPIPSDQ
                     VPPEQE"
     gene            complement(1426..2148)
                     /locus_tag="PKZ74_12160"
     CDS             complement(1426..2148)
                     /locus_tag="PKZ74_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93552.1"
                     /translation="MNSQQFYRWMDDPTALDSESLAELQQVAAVFPYFSTGRILYLLN
                     LKKLDDYRFEQELKNAAVFISDRSRLRGWINFLDDNENKNGGQTEILPLEPEQDDSLR
                     EIENQIRDNIREIEQKQMRLKELMEEKKTLLNMESGVPEKDKEQGNLPRPLPKDDLLE
                     DFLLQQQSHPSNKKSFYNPEDYARKSIEENDGIFSETLARLVAAQGKKEKAVKIYQQL
                     MLKYPQKSSYFAAQIEKLKKES"
     gene            complement(2165..2674)
                     /locus_tag="PKZ74_12165"
     CDS             complement(2165..2674)
                     /locus_tag="PKZ74_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPM93553.1"
                     /translation="MIFRNITGIFLLTAVMLTAGCGVYSFTGASIPPEAKTISISYFV
                     NNAQYVEPTLSQSLTDALRDRFQSQTSLTFIAEDGDLQLEGSITDYSTKPMAIQGNET
                     AALNRLSVTIKVRFTNSIDPAKDYEASFTRFEDYSSNQDLSAVKDQLIPLINEALVDD
                     IFNKAVVNW"
     gene            complement(2661..3899)
                     /locus_tag="PKZ74_12170"
     CDS             complement(2661..3899)
                     /locus_tag="PKZ74_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM93554.1"
                     /translation="MYRMEVQAIKQRFGIIGNSSLLIRSIDIARQVAPTDLTVLITGE
                     SGTGKEVFPQIIHQLSARKHGAYIAVNCGAIPEGTIDSELFGHEKGSFTGAVEARKGY
                     FEEANGGTIFLDEVGELPLSTQVRLLRVLESGEFIKVGSSKVLKTNVRLVAATNVNLE
                     NAIRQGKFREDLFYRLNSVPINVPPLRDRKEDIYLLFRKFVADFAEKYRMPAIELTDE
                     ARLMLENYRWPGNVRQLKNITEQISIIEQNRRITPESLRRYLPEGSFNELPVLYQGEM
                     KDASFSERDLLYKVLFDMKKDIHDLKKIVADILQDEKSPAKIEDSQPHFFAHPEEVKV
                     SIHPPDKTIPINDHFTDEFEDSIQDSEVVEESLSLQKKEIDMIRKALEKYNGRRKNAA
                     RELGISERTLYRKIKEYDIS"
     gene            complement(3987..>4673)
                     /locus_tag="PKZ74_12175"
     CDS             complement(3987..>4673)
                     /locus_tag="PKZ74_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HPM93555.1"
                     /translation="FPGPGIGGYCLPKDGGLGYWSYRHILGFEDGDDIFQITPTAIDI
                     NDTRSLHVAELTRDALRNMSRYIAGSRVLVCGASYRQDVGDTRYSGSEIVVRKLTEMG
                     AEISVHDPFVDHWYELETQDTYPAPGISWKRFFRNQEHLTEIRVQKDLKKSLKGTDGV
                     ILAVPHEPYMNLSPDNIVKWAGNPVAVIDCFGILTDDQIRRYFELGCEVKALGRGHIQ
                     RIKKEVQKGK"
BASE COUNT         1106 a         1148 c         1093 g         1326 t
ORIGIN      
        1 cgcttccatt ttctcggtat cggtcacaaa gtaagcagag atgtaaccgc ggtcgaactg
       61 cattccttcc accacatcga cataggtttc catacttttg gcttcctcga tcgtgatcac
      121 gccctccttc ttcactttgg ccatagcttc ggcaatcagt ttaccgatgg caaagtcgtt
      181 attggcagaa atagtggcta cctgctcgat tttctcgaag ctgtcgccga tctcttttgt
      241 ctgttctttc agggatttga tgaccttttc aacagccagg tcgatacccc tttttatttc
      301 catcgggttg gcgccggccg tgacattttt cagtccggtc gtaaagattg cctgagcgag
      361 gacagtagcc gtggtagtac catcaccggc aaggtcgctg gttttggaag ccacctcttt
      421 caccatctgg gcgcccatgt tctcggcatt gtctttcagt tccacttctt tggcaacggt
      481 aacgccgtcc ttggtaatgg tcggggaacc gaatgatttc tgtatgatga cattacgtcc
      541 tttcgggccg agggtcactt ttactgcatt ggatagttca tcgacacctt tcttcagttt
      601 ttctcttgcg gccaggtcga aaatgatttc ttttgccatg atgttcagat atttaagttt
      661 aaattaataa atttcaatta gataacagca acgatgtcgg attcgcgcat gatcaggaaa
      721 ttttttccct catagctaaa ttcagtgcct gcatattttg catagagaac ctcatcgcca
      781 actttcacag taagaggttc gtctttctta ccggggccca cggccacgat ggttccgcgt
      841 tgtggttttt cctttgctgt gtcgggaatg ataatccctc cggctgtctt ttcctctgca
      901 attgcgggtt caacgattac ccggtctgct aacggtttaa tgctgattgt tgtcatataa
      961 atagagtttt tagattatta aaattccgct caggtttgtc ataatttatg ccaaaccgga
     1021 taaaaaaaag aaatgtcaga atcttatgac attctgacat ttcataaaaa aaatatgttc
     1081 aaacaatcat tcctgttcag gcggaacctg gtctgacgga atgggttgca tgttctcgat
     1141 cttttcacga agttccgtat cctgcgtact tccggttgtt gcattgcggg gaatgaccag
     1201 gatggagatc aggctgaggg ccagaagggc aatagccagg ctccaggttg ttttttcaag
     1261 aaaatcggcc gttttacgga cacccatgat ctgaccggtc gaaccgaaat tggaagccag
     1321 gccgccacct ttggaattct gaactaaaac gacgagtatc aggagaatac aagcaactaa
     1381 gaccagcacc gaaactaaaa tatataaacc catttcgcta aatttttatg attctttttt
     1441 gagtttttca atttgggctg caaagtaact acttttttgg ggatatttca acattagttg
     1501 ctgataaatt ttaaccgcct tttctttttt gccctgggcg gcaaccagac gggccagggt
     1561 ttcgctaaaa ataccgtcgt tttcctcaat actcttcctg gcgtaatcct caggattgta
     1621 aaaagatttt ttatttgaag gatgcgattg ttgttgcaac aggaaatcct caagcagatc
     1681 gtcttttggt aaagggcggg gcaggttacc ctgctcctta tctttctccg gaacgccgga
     1741 ttccatgttc agaagcgtct ttttttcttc catcagctcc ttcaggcgca tttgtttctg
     1801 ctcaatttca cggatattat cccggatctg attttcaatt tcccgcaacg aatcatcctg
     1861 ctcaggttcc agcggcagaa tctctgtctg gccgccattc ttattctcat tatcatccaa
     1921 aaaattgatc cagcccctta gccgggaccg gtccgaaatg aagacagctg catttttcag
     1981 ctcctgttca aaccggtaat catccagttt cttaaggtta agcaggtaca ggatacggcc
     2041 tgttgagaaa taggggaaaa cggcagccac ctgttgcaat tcggccaggc tctctgagtc
     2101 gagagcggtg gggtcatcca tccaacggta aaactgctga ctattcataa gcacaatttt
     2161 aagcttacca gtttaccacg gctttattaa aaatatcatc caccagcgct tcgttgatga
     2221 gaggaatcaa ctggtctttg accgcggaga ggtcctggtt gctggaataa tcttcaaacc
     2281 gtgtaaaact ggcctcataa tcctttgccg gatcgatcga attggtgaac ctaactttga
     2341 tcgtgacaga aagcctgttc agtgcagccg tttcatttcc ctggatagcc ataggtttag
     2401 tggaataatc ggtaatggaa ccttcaagct gcaaatcacc gtcttcggca ataaaagtga
     2461 ggctggtctg cgactggaac cggtcacgaa gggcatcggt caggctctgg ctcaacgttg
     2521 gctcgacata ctgggcatta ttcacaaaat agctgattga gatggttttc gcttccgggg
     2581 gaatgctggc gccggtaaac gaatagacac cgcagccggc cgtcagcatg actgcggtca
     2641 gcaggaaaat gccggtaatg ttacgaaata tcatattcct tgatctttcg gtagagggtc
     2701 ctctccgata tacccagctc acgtgcggca tttttccgcc gtccgttgta tttttccagt
     2761 gctttccgga tcatgtcgat ctcctttttt tgcagggaga gcgattcttc cactacttcc
     2821 gaatcctgga tggaatcctc aaattcatcc gtaaaatggt cattgatagg tatcgtttta
     2881 tcgggtggat gaatactgac ctttacctcc tccggatggg caaaaaagtg cggctgtgaa
     2941 tcttcaatct ttgccggtga tttctcatcc tgaaggatat cggccacaat ctttttcagg
     3001 tcatggatgt ctttcttcat atcgaacaga accttataaa ggagatcgcg ttcggaaaaa
     3061 gaggcatcct tcatttcgcc ctggtaaagc acggggagtt cgttgaacga tccttcgggc
     3121 agataacggc gaaggctttc aggggtaatc ctgcggtttt gctcaatgat agaaatctgt
     3181 tcggtgatgt tcttcagttg cctgacattg cccggccagc ggtaattttc aagcatcaga
     3241 cgtgcttcgt cggtcagttc gatagccggc atcctgtact tttcagcaaa atcggccaca
     3301 aatttcctga acagcaggta aatatcctct ttccggtctc tcagcggcgg cacattgatg
     3361 ggtacgctgt tcagccggta gaaaaggtct tccctgaatt ttccctgccg gatggcattc
     3421 tcaaggttga cattggtggc ggcaaccagc ctgacatttg ttttcagtac tttagacgag
     3481 ccgactttga taaattcgcc cgattccagt acccgcaaaa ggcgtacctg tgtggacagg
     3541 ggcaattcac cgacttcatc caggaagatc gtacctccgt tggcttcttc gaaatagcct
     3601 ttccgggctt ccacggcgcc ggtaaacgac cccttttcat ggccgaacaa ttcgctgtcg
     3661 atagtcccct cggggatagc gccgcagttt acggcaatat aggcgccatg cttcctggcg
     3721 ctgagttgat gaatgatctg cggaaaaact tctttacccg taccactctc cccggtgatc
     3781 agtactgtca aatcggtcgg ggcaacctgc ctggcaatat cgatcgaccg gatcagcagc
     3841 gacgagtttc cgataatgcc aaagcgttgc ttaatagcct gaacttccat tctgtaaatc
     3901 ccaataatac tctggtacaa aggtactatg aaaatctgac atgaaatcac ccgcaggcaa
     3961 gaagaaaaga caatctgtca gaagtgctat tttcccttct gtacttcttt cttaattcgt
     4021 tgaatatgac ctcgtccgag ggccttaacc tcgcaaccga gttcaaaata gcgcctgatc
     4081 tggtcgtctg tcagaatgcc aaagcaatcg atgacggcga cgggattacc ggcccatttc
     4141 acgatgttat ccggcgacag gttcatatac ggttcatgcg gtacggcaag gataacgcca
     4201 tcggttccct tcagcgactt tttgaggtct ttctgaacgc ggatttctgt caggtgttcc
     4261 tggttcctga aaaatctttt ccaggagatg ccgggagccg ggtaagtatc ctgggtttcg
     4321 agctcgtacc agtgatcgac gaacggatcg tggacactga tctcggcgcc catttcggtt
     4381 aatttcctca cgacaatttc cgaaccgctg taacgggtat cgcccacatc ctgccggtag
     4441 ctggcgccgc acaccagcac cctggagccg gcaatgtagc gcgacatatt ccggagggca
     4501 tcgcgggtca gttccgccac atgaagcgag cgggtatcgt taatatcgat ggccgtaggg
     4561 gtaatctgga atatgtcatc gccatcttcg aatcccagga tatggcggta cgaccagtag
     4621 ccaagcccgc cgtctttcgg aaggcagtag ccgccgatac cgggtcccgg aaa
//
LOCUS       DAOPNH010000265         4669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21452_length_4669_cov_6.111183, whole genome shotgun sequence.
ACCESSION   DAOPNH010000265 DAOPNH010000000
VERSION     DAOPNH010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21452_length_4669_cov_6.111183"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKZ74_12180"
     CDS             complement(<1..705)
                     /locus_tag="PKZ74_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPM93556.1"
                     /translation="MEISNEVIFFSGFIVLILFLLFIDLGVFSKNSHIVSLRESVSWT
                     ILWVSLSIGFYFFIRHYGNLIHGYDSIADIQASIDKFKHPINIEGLTFDQALQVFNKN
                     LSIQYITGYIIEYSLSLDNVFVILLIFLSFNVPEKYYKRVLFWGILGAIIMRFIFIFS
                     LSAIIYKFSWVMYIFGLFLVFTAIKMFLDFIKKKEEKIDVEHHPMVRLTKRFFPVHPK
                     FEGNKFWIRKNRIVYVT"
     gene            839..2341
                     /locus_tag="PKZ74_12185"
     CDS             839..2341
                     /locus_tag="PKZ74_12185"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPM93557.1"
                     /translation="MKANTEMNPNPLVQYLNKPAEEFTRQDIIKYVEDNNIEMINFRY
                     VGWDGRLKTLNFIINGRDHLETILTMGERVDGSSLFSFIEAGSSDLYVIPKYRTAFIN
                     PFTEIPTIDIICSYFNKDGEPLESSPEYILKKADRALKEKTGYELEVMGELEYYIISP
                     KDDLFQAVDQRGYHESTPFTKWEHLRSEAMQAIAQAGGQIKYGHSEVGNFEVGDLMYE
                     QNEIEFLPCPLESAAEQLLIAKWILRSLAYKYGVTISFAPKITLGKAGSGLHIHTRLM
                     KDGKNVMIENGRLSDTARRMIAGFLDLAPSLTSFGNINPTSYFRLVPHQEAPTNICWG
                     DRNRSVLVRVPLGWTGKANMVQLANPLEEAPHSTLTEKQTVEYRGADGTADIYLLIAG
                     LAVAALHGLEMKDSLKYAERTYVDVNIFKDEHKDRLKGLSQLPTSCWESALALEKQKP
                     LYLKYGIFSEGMIDWIIRYLKSFDDQHLRRDIGQNEAEIMKLVQKYFHCG"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2717..3142
                     /locus_tag="PKZ74_12190"
     CDS             2717..3142
                     /locus_tag="PKZ74_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM93558.1"
                     /translation="MNYMRISHDPVMRMIMKDFMNQERETERKCRWTPATNITENTDS
                     FRIEMAVPGYNKEDFRIDLEKDVLTISGVIEKEENQEAKPEPNYRMREFARRNFCRSF
                     TVNETIEKDSIKAEYNNGILTVVLPRREEVKVSKEIRVE"
     gene            complement(3309..3818)
                     /locus_tag="PKZ74_12195"
     CDS             complement(3309..3818)
                     /locus_tag="PKZ74_12195"
                     /inference="COORDINATES: protein motif:HMM:NF006739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPM93559.1"
                     /translation="MTQKRIGIFYGSSTGQTEAVAEKLQRIIGEDQADLINVDIATET
                     DIEKYPFLIFGTPTWGVGEMQDDMEDFAEILAKADLKGKKIALFGLGDQDTYPDSFAD
                     GVGMLYEKIKDRANIVGFWPKNGYVFHESEAFRDNHFVGLIIDQENQSAKTNERLEKW
                     VEMLKKEFV"
     gene            4231..4569
                     /locus_tag="PKZ74_12200"
     CDS             4231..4569
                     /locus_tag="PKZ74_12200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM93560.1"
                     /translation="MSIKNLYLYQANKTFMPPDLSDVFVNHNFPRNRFEVDLDDDHQA
                     VLIYMIKAGLFVMLHTEVPPQFEGRGIAGKMAKEALQFAAREGLKVRSYCTFTTRYIE
                     RHPEYHYLLG"
BASE COUNT         1335 a         1043 c          988 g         1203 t
ORIGIN      
        1 cgttacgtaa acgatcctgt ttttccttat ccagaattta ttcccttcaa acttcgggtg
       61 aaccgggaag aatcttttgg tcagcctgac catcgggtgg tgctccacat caatcttttc
      121 ttctttcttt ttaatgaaat ccagaaacat ctttattgcg gtgaaaacca ggaagagccc
      181 gaagatatac atcacccatg aaaacttgta aataatggcc gataaggaaa agatgaaaat
      241 gaaccgcata atgatggctc ccaggatccc ccagaacagg acccgcttgt aatatttctc
      301 cgggacattg aaagagagga agatgagcag aatgacaaat acattatcca ggcttaaaga
      361 atattcgatg atataacctg taatgtactg gatcgataaa tttttattaa aaacctgcag
      421 tgcctggtca aatgtcagcc cttcgatatt tatcgggtgc ttgaacttat ctatgctggc
      481 ttgtatatcg gcgatggaat catacccgtg tatcaggttt ccgtaatgcc ggataaaaaa
      541 gtagaacccg atcgaaaggc ttacccacag tatcgtccat gataccgact cccgcagcga
      601 tactatgtgg ctgttcttgc tgaaaacccc gaggtcgatg aataaaagga aaaggattaa
      661 tacgataaat cctgaaaaaa aaataacctc gttgctgatt tccatcttga cacaaaaatt
      721 tcaacaaaat tagttattgt tttgaaacaa tctaatttac atggccggcc ctttttatag
      781 ttcaaaaatt tatatgtttg ccgtcctttg ttaaatacta attataaaga tgataataat
      841 gaaagcgaat accgaaatga atccgaatcc gctggtgcag tatctgaata aaccagccga
      901 ggagtttacc cgtcaggata tcatcaagta tgtggaagac aataatatag agatgataaa
      961 tttccgatat gtcgggtggg atggaagact gaaaacactg aactttatca ttaacggccg
     1021 ggatcacctg gaaaccattt tgacaatggg tgagagggtc gatgggtcga gccttttctc
     1081 ttttattgaa gccggatcga gcgacttgta tgttattccc aaatacagga cggcatttat
     1141 taaccctttc acggaaattc caactatcga tattatatgt tcctatttta acaaagatgg
     1201 cgagcctctg gaaagcagcc cggagtatat cctgaagaaa gccgaccggg ccctgaagga
     1261 gaaaacaggg tatgagctgg aggtgatggg cgagctggaa tactacatca tcagcccgaa
     1321 agatgatctc ttccaggcag tcgaccagag aggctaccat gaatccacac ctttcaccaa
     1381 gtgggaacat cttcgttccg aagctatgca ggccattgcc caggcgggcg gacagatcaa
     1441 gtacggccat tcggaagtcg gcaattttga agttggtgac ctgatgtatg agcagaacga
     1501 aatcgaattt cttccctgcc cgctggaaag cgcagcagaa caactactca tcgcaaaatg
     1561 gatcctccgg tcgctggctt acaaatatgg cgtcacgatc tcatttgcgc ccaagatcac
     1621 cctgggaaaa gcgggcagcg gcctacatat ccatacccgg ctgatgaagg acgggaaaaa
     1681 tgtgatgatc gaaaatggac ggctgagcga tactgcccgg agaatgatcg ccgggttcct
     1741 ggaccttgcc ccgtcgctca cctcgttcgg gaacatcaat ccgacttcgt atttccgcct
     1801 ggtgccgcac caggaggcgc cgaccaacat ttgctggggc gaccggaacc gctcggtgct
     1861 ggtcagggtg ccgctcggat ggaccggtaa ggccaacatg gtgcaactgg cgaatccgct
     1921 ggaggaggcg ccgcattcaa ccctcaccga gaagcagact gtggaatacc gcggcgccga
     1981 cggcacggcc gacatctacc tgctcatagc aggcctggct gtggctgccc tgcatggcct
     2041 cgaaatgaag gattcgctta agtatgccga acggacgtat gtcgatgtca atatctttaa
     2101 agatgagcac aaagaccgtc tgaaggggct gtcgcagctt ccgacctcct gctgggaatc
     2161 cgctctggcg ctcgaaaaac aaaaaccact gtacctgaaa tacggtattt tctccgaagg
     2221 catgatcgac tggatcatcc gttacctcaa atcgttcgac gatcagcatc tgcgtcgcga
     2281 cataggccag aacgaagcag agatcatgaa gctggtgcag aagtatttcc actgcgggta
     2341 atggggtctg gttattttca gataaaagag gctgtatcaa cagtagtaaa ccacaaagga
     2401 atactaagga tttcaccaag gcgcacaaag attatnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnngacat taatacaccc tctttatttt caaacagaac acaaaaatat
     2581 cagccagaat ggcattctga atcatcggaa aagcgtcatt atggcatatt tttagcaatt
     2641 ttctcccccg gaacaattct tgacaaaacc ccatctcata aatccataga gaattaaaca
     2701 ttaaaaatta agcattatga attatatgag aatatcccac gaccccgtta tgcggatgat
     2761 tatgaaagac ttcatgaacc aggagcgcga aaccgagcgc aaatgccgct ggacacccgc
     2821 aactaacatc accgagaaca ccgattcctt ccggatcgag atggctgttc cgggttataa
     2881 caaagaagat ttccggatcg accttgaaaa ggacgttttg acaatttcag gggtgattga
     2941 gaaagaggag aaccaggagg cgaagccgga acccaactac aggatgcgcg aatttgcccg
     3001 ccggaacttc tgccggtcat ttacagtgaa tgagaccatt gaaaaagatt ccatcaaagc
     3061 cgaatacaac aatggcattc tgacggttgt cctgcccagg agagaagagg tgaaagtgtc
     3121 gaaagagatc agggtcgagt aaaaaacctc actcaatata tagttgtttt cataaatgaa
     3181 agaaccggtt tatgaccggt ttttttttat aatgagatgg catctcatat ttcaagaaag
     3241 acttgctaat aaccttatct gcgttgtaaa agcaaactat tgcaagtgga tttgagatgc
     3301 catctcaatt aaacaaattc cttcttcagc atctccaccc atttctcaag ccgttcgttg
     3361 gttttggccg actggttctc ctgatcgatg atcaggccga cgaaatggtt gtcgcggaaa
     3421 gcttccgatt cgtgaaatac atacccgttt ttaggccaga aaccaacgat atttgcccgg
     3481 tctttgatct tttcatataa cataccaacg ccgtcggcga aactgtcggg ataggtatcc
     3541 tggtctccca ggccaaacag ggctattttc ttccctttca ggtcggcttt ggcaaggatt
     3601 tctgcaaaat cttccatgtc gtcctgcatc tccccgacac cccaggtagg ggttccaaaa
     3661 atcagaaacg gatatttttc tatatctgtt tccgtggcaa tatcaacatt gattaggtct
     3721 gcctggtctt caccaattat tctttgcaat ttttcagcaa ccgcctctgt ttgcccggtg
     3781 ctggatccgt aaaagatccc gattcttttc tgtgtcattt ctaaacagtt gaaatcagcg
     3841 gcaaaaatac gatttattat ttttatgtag atcgaatata aataatttcc ctgtcagtta
     3901 tacagggaag aacaactaca ttgaatacgc aaataccgaa aaatcggtac aggctctgtt
     3961 tagactcggt ataacaccga attggtcgtt cctttgggca tttcatcgct tagttttcca
     4021 gcaattctta ccctgccggg aattgaatca tgcctgaact gcctgtgact tcccttctgt
     4081 gaaacaagat accatcccct gctctcaatc aatttaatga tctctttcac tttattcccc
     4141 accaatattt tacaagggta atacaagtaa taccaattta tgtgatttat ttcatcattt
     4201 catgtatacc aattttttaa tatcgcatga ttgtctatca aaaacctata cctttaccag
     4261 gctaataaaa cctttatgcc ccctgatctt tctgatgttt ttgtcaatca taacttcccc
     4321 cgcaaccggt tcgaagttga tctcgatgat gaccaccagg ctgtcttgat ctatatgatc
     4381 aaagccggct tgttcgttat gttgcatacc gaagtgcctc cacagtttga aggcagggga
     4441 atcgcaggga aaatggcaaa agaggcgctg caatttgcgg ccagggaagg cctcaaagta
     4501 aggtcctatt gcacctttac cacccggtat atcgaacgcc accctgaata ccattacttg
     4561 ttgggctaaa gatccaggat tgtaatttgt aatttgaaaa tcttagatta taaaatgaac
     4621 ctaacgtata ccattaaaat ttcctaatat tgtggaaatg tacgttttc
//
LOCUS       DAOPNH010000266         4631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21700_length_4631_cov_6.341565, whole genome shotgun sequence.
ACCESSION   DAOPNH010000266 DAOPNH010000000
VERSION     DAOPNH010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21700_length_4631_cov_6.341565"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PKZ74_12205"
     CDS             complement(<1..1353)
                     /locus_tag="PKZ74_12205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPM93561.1"
                     /translation="MNKKLQVIRYLLADLFSAALAWSFFFIYRKYSIEPVTFENLKTI
                     LQDTNYFLGVVIVPAFWIFLYFILGTYNENKIFRKSRLREMSQTLFISLVGVIIIFFA
                     LILDDAISTYKSYYQLFAVLYLLHFGFTYLFRLLLTSRIVRKIHKKQLGFNTIIVGSN
                     GNAVKIFEEIEHQELPSGNKFIGFVSVKDYDHFKVARYLPYLGKFQDLKRIIAENQVE
                     EVILAIERSERETIRTILTMLEETDVVIKIIPDMQDYLMGMVKMSSIWHTPLIQVSQD
                     LMPPWQQSIKRFIDIIAALIALIILFPVFVIIAIGIKIGSKGPIIYSQERVGLHGHAF
                     KMHKFRSMYSDAERHGPQLSKKDDSRITRIGKFLRKVRLDEVPQFYSVLKGDMSLVGY
                     RPERQYYIDKIVEKAPEYRLLFKIKPGITSWGQVKYGYAENVEQMVERLKYDLLYIEN
                     M"
     gene            complement(1353..2273)
                     /locus_tag="PKZ74_12210"
     CDS             complement(1353..2273)
                     /locus_tag="PKZ74_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93562.1"
                     /translation="MRSLNRKASLPNILIWFLFSIFIITLTGCDKFEGDQTVPSYLKI
                     DSILFISDNELEGTSNQKISDAWVFIDDDLIGGFELPALIPILKKGNHKLEVRPGIIL
                     NGISDTRAPYPCIQPVIIESLELFPDSIVTLSLTSNYYENSEFVWMEDFEDASLAIKA
                     SQNSDTAIYRTNGNLAYHDVYSEFSGICYVDTANSFVQLVSVESNNQGFVFDRGDFIF
                     LEINYRNNIPIVVGVYIKLMDNTVQQRSFLIINPTEEWNKIYVNFTPIVNETTDAVYY
                     NLYLEAQQQDDVEKGYIMLDNIKLVTRPNL"
     gene            complement(2318..3304)
                     /locus_tag="PKZ74_12215"
     CDS             complement(2318..3304)
                     /locus_tag="PKZ74_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM93563.1"
                     /translation="MVKIGVLGAGHLGKIHIKCIRDIKNFELVGFHDADVKTASNVKS
                     EFGLYHFENIEELIDSVDVVDIVTPTVSHFDCASRALKKARHVFIEKPVVTSPEEARQ
                     LMVYADEANVKVQVGHVERFNPAFLAAQPFINKPMFIESHRLAQFNPRGTDVPVVLDL
                     MIHDIDIVLSAVRSNIKKINASGVSVVSDSPDIANARIEFDNGCVANLTASRISMKNM
                     RKSRFFQRDAYISVDFLKKDVEIIRISDPQDENPDPLAMIIDLGAKRGKKQISIEKPE
                     VQNINAIKTELESFHDAIVNNTTPPVTINDGYEALSVAYQILDKMKELPVGL"
     gene            3420..4070
                     /locus_tag="PKZ74_12220"
     CDS             3420..4070
                     /locus_tag="PKZ74_12220"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPM93564.1"
                     /translation="MIQDTFRQKGLRKKLVDILRDKGISDERVLNAINRVPRHLFLDS
                     SFLEFAYQDKAFPIGSGQTISQPYTVAFQTELLQVREGDKILEIGTGSGYQSCVLIEM
                     GAKVFTIERQKNLFDRTKAFLPGIGYFPKMFYGDGYKGLPSFAPFDKILITAAAPGIP
                     DELIRQLKNDGIIVAPVGSGESQTMMAMEKINDQQKKISEHGKFRFVPMLNKKAKD"
     gene            4186..>4631
                     /locus_tag="PKZ74_12225"
     CDS             4186..>4631
                     /locus_tag="PKZ74_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93565.1"
                     /translation="MKKYLYFIGAFVIASSLFLSSCTEDEDPVDLPPSISFVVEDGFI
                     SGNTTLTINTPFNVKLLAVANATSASKIASIEINRIYNNQTIWDTTLTYNDATVNLTV
                     GFVALANAGVENIEFIATDKDGQTDKINLQITTEEPYGGPTSLWEHR"
BASE COUNT         1298 a          990 c         1005 g         1338 t
ORIGIN      
        1 catattttcg atatacagca ggtcatattt caatcgttcc accatctgct ccacattctc
       61 cgcataaccg tatttaacct ggccccagga tgttatgccc ggtttgattt taaacagcag
      121 cctgtattcg ggtgcctttt ccacgatttt atcgatgtaa tactgccttt cgggtctgta
      181 tccgaccaac gacatatctc ctttgagcac cgaatagaac tggggtactt catcgagcct
      241 gacctttctg aggaatttac cgattcgggt aatcctgctg tcgtctttct ttgaaagctg
      301 cggcccatgt ctctccgcat cggagtacat cgacctgaat ttatgcattt taaatgcatg
      361 cccatgcaat ccgacccgct cctgggaata aatgatcggc cctttggagc caattttgat
      421 tccaatggca ataatgacaa atacagggaa taaaatgatc agggctatca gggccgcaat
      481 aatatcgatg aagcgtttga tggattgttg ccagggaggc ataagatcct gagatacctg
      541 gatcaggggc gtatgccata tggatgacat cttgaccatg cccatcaggt aatcctgcat
      601 gtcgggaata atcttaatga ccacgtctgt ctcctccagc atggtcagga tcgtccggat
      661 ggtttctctt tccgaccgct caatcgcaag gattacttct tcaacctggt tttcggcaat
      721 aattctctta agatcctgaa atttacccag gtaaggcagg tacctggcaa ccttaaaatg
      781 gtcgtaatcc ttgacgctga caaagccaat gaatttgtta ccagaaggca gttcctgatg
      841 ttcaatttct tcaaatattt tcacggcatt cccattgctc cccacaataa tcgtattaaa
      901 gccaagctgc tttttatgaa ttttccttac tatccggctt gtaagtaata aacggaagag
      961 gtatgtaaag ccgaaatgta atagatataa gacggcaaat aactgataat atgacttata
     1021 ggtgcttatg gcatcgtcca ggataagggc aaaaaatatt attatgactc cgaccagtga
     1081 aataaagagt gtctggctca tttcccgaag cctggatttc cggaagatct tattttcgtt
     1141 gtacgtgccg aggatgaaat acaggaaaat ccagaacgca gggacaataa ccacaccgag
     1201 gaaatagttc gtatcctgta agatcgtctt caggttttcg aatgtaaccg gttcaattga
     1261 atacttacga taaataaaaa agaatgacca ggccagcgca gcggagaaga ggtcggccag
     1321 aagatacctg attacctgta attttttatt catcaaagat ttggtctggt taccagttta
     1381 atattatcca gcatgatata acctttttca acatcatcct gttgttgagc ttcaagatat
     1441 aaattataat aaactgcgtc tgttgtttca ttgacaatcg gtgtaaagtt aacatagatt
     1501 ttattccatt cttcagtcgg gtttattatc aggaatgacc tctgctgcac cgtattatcc
     1561 ataagtttga tatatacccc gacaacaatt gggatattat tccggtagtt tatttccaga
     1621 aagatgaaat ctccccggtc gaaaacaaat ccctggttat tgctttccac tgagaccagt
     1681 tggacaaaac tattcgccgt atctacatag caaattcctg aaaattcgga gtatacatca
     1741 tgataagcca gatttccatt tgtccggtat atggccgtat cactgttctg gctggctttg
     1801 atggccaggc ttgcatcttc aaaatcttcc atccagacga actccgaatt ttcatagtaa
     1861 ttggatgtta atgacagggt tactatgcta tcggggaaaa gttctaacga ttcgatgatt
     1921 accggttgga tacagggata gggcgcccgg gtgtcggaga taccgttcaa tatgataccg
     1981 ggacggacct ccagcttatg attacctttt ttgaggattg gaatgagtgc aggtagctcg
     2041 aacccgccga taaggtcatc gtcgataaaa acccatgcat cggaaatctt ctggttgctg
     2101 gtcccctcta gctcattatc ggaaataaat aaaatggaat cgatcttcag gtatgatgga
     2161 accgtctgat ccccttcaaa tttatcgcat cctgtaagag ttatgataaa tatagaaaac
     2221 aggaaccaga tcagaatatt cggcaggctt gcttttcggt ttagacttct catgtttatc
     2281 caacggtgac aggcacgtga ttattgcttc gtttcggtta cagacccacg ggtaactcct
     2341 tcattttatc gaggatctgg taagctacgc tcagggcttc gtagccgtca ttaatagtaa
     2401 ccggaggggt tgtattgttg acgatggcat cgtgaaaact ttcgagttca gttttaatgg
     2461 cattgatatt ctgtacttcc ggtttttcga tggagatttg ttttttcccc cttttggcgc
     2521 ccagatcaat aatcatagcc agaggatcgg ggttttcatc ctgagggtcg ctgatccgga
     2581 tgatttctac gtctttctta agaaaatcga cggagatata tgcatcgcgc tggaagaaac
     2641 gggatttccg catattcttc atagagatcc ggctggctgt caggttggct acgcaaccgt
     2701 tatcgaattc tatcctggca ttggcaatgt cgggtgaatc gctgacaacg gaaacgccac
     2761 ttgcattgat cttttttata ttcgacctca cggcgctgag tacgatatcg atatcatgaa
     2821 tcatcaggtc gagcacaacg ggcacatccg ttccacgagg attaaactgg gccaggcggt
     2881 gtgattcgat gaacatcggt ttgttgataa aaggctgtgc ggcaagaaat gccgggttaa
     2941 accgttcgac atggccaacc tgcaccttca cgttggcttc gtcggcgtat accataagtt
     3001 gccgggcctc ttccggtgag gttacgacgg gcttctcgat aaaaacatgc cgggcttttt
     3061 tcagtgccct ggaagcacaa tcaaaatgag aaacagtcgg agtgacaata tcgacaacat
     3121 caacagaatc aataagttct tcaatattct caaaatgata aagaccgaat tcggatttta
     3181 cattggatgc cgttttaaca tctgcgtcgt gaaatccgac aagttcaaaa ttcttaatat
     3241 cccggatgca cttaatatgg attttaccca ggtgcccagc tcccaataca ccgattttta
     3301 ccattttgga ttttttgtgc aaatgtagct ttttgaccat aaccatcgaa ggaaaggttt
     3361 gtcatctttt caacggaatt attattttcg cggcaacaag aaggttaatt gttttcatca
     3421 tgatccagga cacatttcgt caaaaagggc tcaggaaaaa attggttgat attttgcggg
     3481 ataaaggcat ctccgatgaa agggtattga atgccatcaa ccgggttccc aggcacttgt
     3541 tcctggattc gtcattcctg gaattcgctt accaggataa ggcatttccg ataggatcgg
     3601 gtcagacgat ctcgcaacct tataccgtcg catttcaaac ggagctatta caggtaaggg
     3661 aaggcgataa aatattggaa attgggaccg gctcgggtta ccagtcgtgt gttttaatcg
     3721 aaatgggagc taaagttttt acgattgagc gtcaaaagaa tttattcgac aggacgaaag
     3781 cgttccttcc aggtatcggt tattttccaa aaatgtttta tggtgatgga tacaaagggc
     3841 tcccctcttt cgcccctttt gataaaatac tcattacggc tgctgcgccg ggcattccgg
     3901 acgaactaat ccgtcaactc aaaaatgatg gcataattgt tgctcctgtc gggtccggtg
     3961 aatctcaaac catgatggcc atggagaaga tcaatgatca acaaaaaaaa atttcggaac
     4021 acggcaagtt cagatttgtg cccatgttaa ataaaaaggc taaggattga ttgattggtt
     4081 aaatttcaac atgtacttag aatgatttta agtaaatttg ctgcggaata acaattaatc
     4141 tgtaatcacg ttaaactcta ttattaacta ataattaaaa agaaaatgaa aaagtatttg
     4201 tatttcatcg gagcgtttgt catcgcctca tcattgtttt tatcctcatg tacggaagat
     4261 gaagatccgg tagatcttcc accaagcatc agcttcgttg ttgaagacgg gtttatttca
     4321 ggcaatacca cgttaacgat caacacaccg tttaatgtaa agttgctggc cgttgccaat
     4381 gcgacctctg catcgaaaat cgcaagcatt gaaattaacc gtatttacaa taaccagacg
     4441 atctgggata cgacgctcac ttataatgac gcaacggtca acctgactgt cggttttgta
     4501 gccctggcga atgccggcgt tgagaacatc gaatttatcg ctaccgataa ggacggacag
     4561 actgacaaga tcaaccttca gatcacgacc gaagaaccgt acggtggtcc cacaagcctg
     4621 tgggagcacc g
//
LOCUS       DAOPNH010000267         4626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21737_length_4626_cov_7.154452, whole genome shotgun sequence.
ACCESSION   DAOPNH010000267 DAOPNH010000000
VERSION     DAOPNH010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21737_length_4626_cov_7.154452"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1557
                     /locus_tag="PKZ74_12230"
     CDS             76..1557
                     /locus_tag="PKZ74_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM93566.1"
                     /translation="MQIIELAYVDSVDIQDIVTKTIEQVLKELDPHSAYLSAEELKKS
                     NEQLEGSFEGIGVTFQIYHDTILVISPVPGGPSEKLGILSGDKIVKINGEDATGKDIN
                     NEWVFDRLRGKKGTSVDISIYRGKKDLLDFTIVRDKIPLNSIDASFMATPEIGYIRLN
                     RFSKTSQEEFVQSVTDLKSKGMNKLILDLRGNSGGLLNTAFELADEFLPAGELIVYTE
                     GLHNPRKDFYATSLGNFEKGKLVIIIDEASASASEIVAGAVQDLDRGIVIGRRSFGKG
                     LVQGPFDLSDGSLIRLTTARYYTPTGRSIQRPYENGTEDYYNDFSERFKHGEYVHADS
                     IKFPDSLKYKTRAGRIVYGGGGIMPDIFIPWDSTMFSDYFVELRRKNVLNTFTLDYVE
                     DNRDKINRQYPGFREFNKEFKITPDIIREFKETAEREGVKFDEEGWRASEALILAQIK
                     AFIARNIWDVNAFYEVMSEIDNEYHRAIDLLEDGQVFGILERN"
     gene            1613..2599
                     /locus_tag="PKZ74_12235"
     CDS             1613..2599
                     /locus_tag="PKZ74_12235"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPM93567.1"
                     /translation="MADLSTSYMGLKLRNPIIAGSSGLTSTVDNIQKISENGAGAVVL
                     KSLFEEQINFAINKTFVQNENNFNYPEALDYISHYTRENDLEKYLELIREAKKSVDIP
                     VIASINCVSAAEWVTFAKNIQDAGADALELNIFVLPSDPRRKAEQHEQIYLDILQSVM
                     RQVRIPVAAKVSYYFSGLTNMALQLSWTGIKGLVLFNRFYSPDIDIEKFEVTSGHVFS
                     TEGESTLTLRWIAMLSDKVQCDLAASTGVHDGKGVIKQMLAGAKAVQVASVLYQKGFD
                     HIHVMTKEIEQWMARHDFKTTSDFIGRMSFKEVSNPAAYERVQFMKHFAGIE"
     gene            2865..3200
                     /locus_tag="PKZ74_12240"
     CDS             2865..3200
                     /locus_tag="PKZ74_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93568.1"
                     /translation="MKKRFIFLVIWIILIGLNSCKQNDPIQLDCDAHNYGFIYFQNNS
                     NDTYGIWIDGIFKMEVTGGTVSQAVNISEGNNRKLKAKQLDGYLIWPTEVETSFNVLS
                     CSSYTWQFP"
     gene            3804..4340
                     /locus_tag="PKZ74_12245"
     CDS             3804..4340
                     /locus_tag="PKZ74_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93569.1"
                     /translation="MKRRNVLILMGLFILIIGCTNSLTRSNAKKQILNEMKDEWKTFR
                     IAVSTRFTDGSVEEISTNINVVDLPESSISDLKRIEKILSDANVIVDNDYNPLIKPYI
                     DRDGNNRMWDFIPLGSAKKIEVDGIKTINDNYKKVEFSVFYEWNNLAKELGCTFPLVV
                     KRVIGFEKYDDGWRIDID"
BASE COUNT         1424 a          920 c         1062 g         1220 t
ORIGIN      
        1 ttattcctga cggccacggt atcaacggga cagaacctgg cgaaccggaa agagatccag
       61 aaactgatcc agaccatgca gatcatcgaa ctggcttatg ttgacagtgt ggatatccag
      121 gacattgtca ccaaaaccat cgagcaggtg ctgaaagaac tggatccgca ctcggcctat
      181 ctctcagcgg aagagcttaa aaaatcaaat gagcagcttg aaggcagctt cgaagggatc
      241 ggcgtgactt tccagattta ccacgacacc atcctggtga tctcaccggt gcccggcggt
      301 ccgtccgaaa aactgggaat cctgtcgggt gataaaatcg taaaaataaa tggagaagac
      361 gccaccggaa aggatatcaa taacgaatgg gtttttgacc ggctccgggg gaaaaaaggc
      421 acctcggtag atatatccat ttaccgggga aagaaagatt tgctcgactt caccattgta
      481 cgggataaaa tcccactgaa ttccatcgac gcatccttta tggctacacc ggaaatcggt
      541 tatatccggt taaaccgttt cagtaagaca tcccaggaag agtttgttca atccgtcacc
      601 gatctgaaaa gcaaagggat gaacaagctg atcctcgacc tgaggggtaa ttcaggcgga
      661 ttgctcaaca cggcctttga gctggccgac gaattccttc ccgccgggga actgatcgtt
      721 tacaccgaag ggctccataa tccgcggaaa gatttttatg ctacatcgct cggaaatttt
      781 gaaaaaggca agctggtgat catcatcgat gaagcatccg cttcggccag cgaaatcgta
      841 gccggcgccg tgcaggatct cgacagaggc attgtgatcg gcagaagatc atttggaaaa
      901 gggctcgtcc agggcccgtt cgacctgtcc gacggttccc tgatccgact caccacggct
      961 cgttattaca ctccgacagg ccgctctatc cagcgccctt acgagaatgg aacggaagat
     1021 tactacaacg acttcagcga acggttcaaa catggcgaat acgtgcatgc cgacagtatt
     1081 aaatttcccg attcactcaa atataaaacc agggccggaa ggattgtata tggcggagga
     1141 ggcataatgc ccgatatttt cattccatgg gattctacta tgttttccga ttattttgtc
     1201 gaactgagaa gaaagaatgt actgaacact ttcaccctcg attatgtgga agataaccgc
     1261 gataagatca atcgtcagta ccccggcttc agagagttca ataaagagtt caaaattacc
     1321 cccgacatta tccgcgagtt caaggaaacc gccgaaaggg aaggggtgaa attcgacgaa
     1381 gagggatgga gagcgtcaga agctttgatc ctggcgcaga tcaaagcgtt tattgccagg
     1441 aatatctggg atgtcaacgc attctatgaa gtgatgtcgg aaatcgacaa tgaataccac
     1501 cgcgccatcg atttgctgga agacggacag gtgttcggca tcctggaaag gaattaatag
     1561 attttacttg cctgataaga ttaatgatta aatttgcttc aaaactttaa tcatggccga
     1621 tctctcaaca tcctacatgg gtctgaaatt aaggaatccc atcattgccg gaagttccgg
     1681 attgacaagc acggttgata atatacaaaa gatcagcgaa aacggcgccg gggctgtcgt
     1741 tcttaaatcg ctattcgaag agcagatcaa tttcgctatc aacaagacct tcgtccagaa
     1801 cgaaaacaat ttcaattatc ctgaagcgct cgactacatc agccattata cccgggaaaa
     1861 tgacctggaa aagtacctgg aactgatccg ggaggcaaaa aaaagtgtcg acattccggt
     1921 aattgccagc atcaactgcg tttctgcagc cgaatgggtc acttttgcca aaaatatcca
     1981 ggatgccggc gccgatgcac tggaactgaa tattttcgtc cttccgtccg atcctcgcag
     2041 gaaagctgag cagcatgaac agatctacct tgatatcctc cagtcggtga tgcgccaggt
     2101 gaggatcccg gtagcagcca aggtcagcta ttatttctcc ggcctcacta acatggctct
     2161 ccagttgagc tggacaggca tcaaaggact ggtgctcttt aaccgttttt attcgcccga
     2221 tatcgatatc gaaaagttcg aagtgacttc cggccatgtt ttcagcacgg aaggggagag
     2281 tacgctgacc ctgcgttgga tagccatgtt aagcgataag gtccagtgcg atctcgctgc
     2341 ttccaccggc gttcacgacg gcaagggggt catcaaacag atgctggccg gggccaaagc
     2401 ggtccaggta gccagcgtcc tctaccagaa aggatttgac cacattcatg tgatgaccaa
     2461 agagatagag caatggatgg cccgtcacga tttcaaaacc acttccgatt tcatcggccg
     2521 gatgagcttc aaagaggtca gcaaccccgc cgcctacgaa cgggtgcagt tcatgaagca
     2581 ctttgcaggg atagagtaaa atctcagcaa catcgagacc ggggtgttgg gattttggaa
     2641 ctttggaacg ctttttcaag ttaaagagta aacggtagac tgctgccact aaaacctgat
     2701 aggctatcgt gcttggtcgc attaacagag tttgttcttc atcatatgac ttcattctct
     2761 ttaacctaat actttccaaa actgattaat cagttatcct tcattttctt tggaataata
     2821 tttattttta taccaagtta ataatccgaa aattagtgtt gaaaatgaaa aaacgattta
     2881 tttttctggt tatctggata attctaatcg gcctcaattc ctgtaaacag aatgatccca
     2941 ttcaattaga ctgcgatgct cacaattacg gatttattta tttccagaat aactccaatg
     3001 atacctacgg gatttggatt gatggcattt ttaaaatgga ggttacaggc gggactgtga
     3061 gccaggcagt aaatatttcg gaaggcaaca accggaaatt gaaggcaaaa caacttgatg
     3121 gttacctgat ctggccgaca gaagttgaga cgagtttcaa tgtactttca tgttcatcat
     3181 atacatggca atttccataa aaaaaactgg ctataaagac ggcggcgtaa cagtcggtga
     3241 aattaatcct gctggctgat tttttttaag cgatagcgta agactttggt cactgtttcc
     3301 aggtgaagag tgagcggtga agcgtgaacg gataaaggaa aagggaaaaa cgaaaagggg
     3361 aggggaaaca attctggaac tgtgaaacca ggaatcccgg aacgaacctc cctcccccgt
     3421 gtgggggagg gttagggtgg gggctgaatt cggatagcgg tagactttgg tcactgaaac
     3481 tccgtgtcgt tctgaattgc cgatccttta gccggtttaa gcgatagcgt aagaatttgg
     3541 tcactaggct tgggtaaatg gtgaattaaa aaaggaattg agaaaaagga aaaggagcga
     3601 gttaagatct cagaaacttt tgaatcgtta aaataaaacc gaaaaccaga ttccgtttgc
     3661 taggctaagc aagacttgag gcaatgaaac ttatgtacta ctatattaaa aaaatatagg
     3721 gttgagtatt tcttttaata taaataaaaa ttaattttac tattattcat atgagagaaa
     3781 ttagtcaaac taaaattatt tttatgaaaa gacgtaatgt tttgattctg atgggattgt
     3841 ttatcctaat tattggctgc accaatagtc ttactagaag taacgctaaa aagcaaattc
     3901 tgaatgaaat gaaagacgag tggaaaacat ttagaattgc agtttcaaca agatttacag
     3961 atggatcggt cgaagaaatt agtaccaata ttaatgttgt cgatcttcca gagagttcaa
     4021 ttagtgactt gaaaagaatt gaaaagattc tttcagatgc taatgttatt gttgataatg
     4081 attataatcc tctaattaag ccatatattg accgtgatgg aaataatcga atgtgggatt
     4141 ttattccttt gggatcagct aagaaaattg aagttgatgg tattaaaact attaatgata
     4201 attacaaaaa ggtggagttt agtgtattct atgaatggaa taatcttgcc aaagaacttg
     4261 ggtgcacttt tccattagta gtaaaaagag taataggatt tgaaaaatat gatgatggtt
     4321 ggcgtattga tattgattaa ttgggattga ataataacgt cgaaattgta attgaattag
     4381 tttaggtatc gggggttgat cgttcaattt gaatagtttt tcaccttaat catcttttat
     4441 ggtttccagc aaacgatgta gttatgagaa gttcttcata aatcatgtga aaaaaacacc
     4501 aaacagagat gttgaattct ttaacctcat tataaaggtt aactttacta aactagcaat
     4561 aaaattattg atataatcac tcattacttt gagatcgcta acatgatgta tccatcctaa
     4621 tcatga
//
LOCUS       DAOPNH010000268         4619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_21797_length_4619_cov_5.611963, whole genome shotgun sequence.
ACCESSION   DAOPNH010000268 DAOPNH010000000
VERSION     DAOPNH010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21797_length_4619_cov_5.611963"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /locus_tag="PKZ74_12250"
     CDS             <1..2353
                     /locus_tag="PKZ74_12250"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPM93570.1"
                     /translation="KGIDKFDIGREKFLEYAWEWKEKHGGIILEQLKKLGASCDWDRT
                     RFTMDPALYESVINVFIDLYNKGLIYRGVRMVNWDPRALTALSDEEVIYKEVNSKLYY
                     IRYFIENSNNEFITIATTRPETILGDTAVCVNPNDERYKGLAGKKVLVPLLNRPVPVI
                     ADEYVDMEFGTGCLKITPAHDINDYEIGRKFKLESIDIFNDNGTLGEKAILYVGEDRF
                     VVREKITRELGDKGHIVKIEDYANKVGYSERTDEVIEPKLSLQWFMKMDVLAKPALDA
                     VMNEDVKFYPSKIKNSYRHWMENVRDWCISRQLWWGQRIPAYYLPNGDFIVAKSAEVA
                     LAKARSEFNMPGLKPEDLKQDEDVLDTWFSSWLWPISTFDGIRHPENEDIRYYYPTND
                     LVTAPEILFFWVARMIMAGLEYRGEVPFRNVYFTGIVRDKLGRKMSKSLGNSPDPILL
                     MDHYGADGVRIGMLFMSPAGNDLPFDEALCEQGRNFSNKIWNALRLVKGWQVDPGLET
                     PETNRASTAWFENKLNQTLALIDDHFLRFRISDALMAAYKLTWDEFCSWYLEMIKPEY
                     QKPIDRETYEKTISFFETLMKLLHPFMPFITEDVWHRLADRPTGDDIIIAPFPVAGEY
                     HDELLKHFDREREVIMAIRNERNAKGIPNKDKIHLRVKKNHGQEPDTYFDCVVKKMSG
                     LADLEYVEEKVPGALTIIVGSTEFYIPVEEEKIDIPAEIARLETDLEYARGFLATVMA
                     KLSNEKFVNSAPEKVVEMERKKQSDAESKIRVIEAQLLNLRTK"
     gene            complement(2398..3054)
                     /locus_tag="PKZ74_12255"
     CDS             complement(2398..3054)
                     /locus_tag="PKZ74_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPM93571.1"
                     /translation="MKIKLGTILVIAFLTLSCTAQKGVQVALLKYSGGGDWYANPTSL
                     TNLVRFCNDNLKMGIEAGYATVEAGSAEIFNYPFVHMTGHGNVFFNESEASNLRNYLL
                     AGGFLHIDDNYGMDPFVRPQMKKVFPELDFVELPFDHPVYHQKFNFPNGLPKIHEHDN
                     KTPQGFGLIYEGRLVCFYSYETDLGDGWEDQDVHHDSDATRQKALQMGANLLSYVFQR
                     "
     gene            complement(3154..4485)
                     /gene="metK"
                     /locus_tag="PKZ74_12260"
     CDS             complement(3154..4485)
                     /gene="metK"
                     /locus_tag="PKZ74_12260"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPM93572.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALLDEFLRHDPESKVACETL
                     VTTGLVVTAGEVRTKGYVSIDEVVRNTIKKIGYTKAEFKFDADSCGVISTIHGQSDDI
                     YRGVERGSKTEEEKYRQGAGDQGMMFGFASTEMDNFMPMPIEVAHILLQQLARIRKET
                     NLMPYLRPDAKSQVTIEYNEMNQPVRIDTLVISTQHDEFDKNPDIMQAKIKEDIRNIL
                     IPMALKEMPDRIVRLFDSEYKLYVNPTGNFVIGGPHGDSGLTGRKIIVDTYGGRCAHG
                     GGAFSGKDASKVDRSAAYAARHIAKNLVAAGVAGEVMVQVAYAIGVAEPVGFLVNTSG
                     TAKVKDKNGLIMSDKQISDIAGKLFDLRPYAIIKKFGLQNPVFSPTSTYGHFGRDHYE
                     AEVEVFYEDRDTFRKTVIENEKEVEKIFKKVTFFGWEKLDAVEAIRSAFGI"
BASE COUNT         1197 a         1163 c         1134 g         1125 t
ORIGIN      
        1 taaagggatc gataaattcg atatcggacg ggagaaattc ctcgagtatg cctgggaatg
       61 gaaagaaaaa cacggcggga tcatcctgga gcagctcaaa aaactgggcg cttcatgcga
      121 ctgggaccgg acccgcttta cgatggaccc ggcactctat gaatctgtca tcaacgtatt
      181 catcgatctg tacaataagg ggttgatata caggggtgta agaatggtga actgggaccc
      241 cagggcactg accgctctat cggatgaaga agttatttac aaagaggtca attccaaact
      301 gtattatata agatatttta ttgaaaactc aaataatgag tttattacga tcgcgacaac
      361 ccgtcccgaa actatccttg gcgatacagc cgtttgtgtg aacccgaatg atgagagata
      421 taaaggcctg gccgggaaga aagtcctggt ccctttgctc aaccggccgg tcccggtcat
      481 tgccgatgaa tatgtggata tggaattcgg tacgggctgc ctgaagatca cgccggctca
      541 cgatatcaac gattatgaga tcgggaggaa gttcaagctc gaatcgatcg atatattcaa
      601 cgacaacggc accctgggcg aaaaagccat cctttacgtg ggcgaagacc gctttgtggt
      661 gcgtgaaaag atcacccgtg aacttggtga caaaggccat atcgttaaga tagaagacta
      721 tgcgaacaag gtgggctatt cggaaaggac cgacgaggtt atcgagccca aattgtcgct
      781 ccagtggttc atgaagatgg atgtactggc aaaaccggcg ctggatgcgg tgatgaatga
      841 agatgtgaaa ttctacccgt cgaagatcaa aaattcttac cgccactgga tggagaatgt
      901 gcgcgactgg tgcatcagca ggcaattgtg gtggggccag cgtatcccgg cctattacct
      961 tccaaacggc gattttatcg tggctaagtc cgctgaagta gccctggcca aagcccgttc
     1021 ggaattcaat atgcccggat tgaaaccgga agacctgaaa caggatgagg atgtactgga
     1081 tacctggttc tcatcgtggc tttggccaat ctccactttc gacgggatca ggcaccctga
     1141 aaatgaagat attcgttatt attacccgac caacgacctg gtgaccgccc cggagatcct
     1201 ctttttctgg gtagcccgta tgatcatggc gggattggaa taccgggggg aggtaccctt
     1261 ccggaatgtt tacttcaccg ggatcgtccg cgacaagctg ggacgcaaaa tgtcgaaatc
     1321 gcttggcaat tcacccgatc cgatcctgct gatggaccac tacggagcag atggcgtcag
     1381 gatcggcatg ctcttcatgt cgcccgccgg caacgacctg ccttttgatg aagcattgtg
     1441 cgagcagggg aggaacttca gcaacaagat ctggaacgcc cttcgcctgg tgaagggctg
     1501 gcaggttgat cccggcctgg agactccgga aacgaaccgg gcttcgacgg catggtttga
     1561 aaacaagctc aaccagaccc tggccctgat cgacgaccat ttcctcaggt tccgtatttc
     1621 cgatgccctg atggcggcct acaagctgac ctgggacgag ttctgttcgt ggtacctgga
     1681 gatgatcaaa ccggaatacc agaagccgat cgaccgggag acctatgaaa aaacgatctc
     1741 tttcttcgaa accctgatga agctgctgca tccattcatg ccttttatta ctgaagatgt
     1801 ctggcaccgg ctggccgacc gccccaccgg cgacgatatc atcatcgcac ctttcccggt
     1861 ggcgggagaa taccacgacg agcttttgaa gcatttcgac agggaacgcg aagtcatcat
     1921 ggccattcgg aacgaacgga acgcaaaagg catcccgaat aaagacaaga tccatctccg
     1981 ggtcaaaaag aaccacggcc aggaaccgga tacctatttc gactgtgtcg tcaaaaaaat
     2041 gtcgggactt gctgacctgg aatatgttga ggaaaaggta cctggagcgc tgactattat
     2101 cgtcggttca accgagtttt acatcccggt agaagaggag aagatcgata tcccggcgga
     2161 aattgccagg ctcgaaactg atcttgaata tgcacgcggt ttcctggcaa cggtaatggc
     2221 taaactcagc aatgaaaagt ttgtgaacag cgccccggaa aaagtggttg agatggaacg
     2281 caaaaaacag tccgacgccg aatcaaagat cagggtgatc gaagcccaat tgttgaattt
     2341 acgtacaaaa tgaattacgg taaattgcag gatcgttgca tgcaacgatc ctgcaattta
     2401 ccgctgaaac acatagctaa gcagattcgc ccccatttgc agcgcctttt gccgcgtggc
     2461 atccgagtcg tggtgcacat cctggtcttc ccagccgtcg cccaggtcgg tctcataaga
     2521 atagaaacaa accagccggc cttcgtaaat caacccgaat ccctgcggtg ttttattatc
     2581 atgctcatgg atcttgggca aaccgttggg aaaattgaat ttctgatggt agaccgggtg
     2641 gtcgaacggg agttccacaa aatcgagctc gggaaagacc tttttcatct gcggcctgac
     2701 aaacgggtcc attccatagt tatcgtcgat atgcagaaag cccccggcaa gcaagtaatt
     2761 ccgcaggttt gaagcttcgc tttcgttgaa aaaaacattc ccgtggcctg tcatgtgtac
     2821 gaacggataa ttgaagatct ctgcgctgcc cgcttcaacc gtagcataac cggcctcaat
     2881 gcccatcttc aggttatcgt tgcagaaccg gaccaggttg gtcagcgacg taggattggc
     2941 ataccagtcg cctccgccac tgtacttcaa aagagcaacc tggactcctt tctgagcagt
     3001 gcaggataat gttaagaatg caatgaccag gatggtacct aacttaattt tcatcagttt
     3061 tcaatgaaaa acataaaagg aaaaatgcca tgaaaaatga aaaaggcaaa acgcaaaatg
     3121 taacatttat tgtcaaatga acaatgccag atatcaaatg ccaaacgcgg agcggatcgc
     3181 ttccactgcg tcaagctttt cccaaccaaa gaatgtcacc tttttgaaga tcttttcaac
     3241 ctctttttcg ttttcaatca ctgtttttct gaaagtatcc ctgtcttcat aaaacacttc
     3301 gacctctgcc tcgtaatgat cccggccaaa atgcccgtag gtactggtag ggctgaaaac
     3361 ggggttctgc aagccgaatt ttttaatgat ggcataaggg cgaaggtcaa ataacttacc
     3421 cgcaatatcg gagatttgtt tatccgacat gataaggcca ttcttatctt taacttttgc
     3481 cgttccgctg gtattcacca ggaaacccac cggttcggcg acaccgatcg cataagccac
     3541 ctgcaccatg acctctccgg caacacctgc cgcaacgagg tttttagcga tgtgtcttgc
     3601 tgcatacgct gccgaacggt cgactttcga ggcgtctttc cccgaaaatg ccccgccccc
     3661 gtgggcgcaa cggccgccgt aagtatcgac aatgatcttt cttccggtca agcccgagtc
     3721 gccgtgcggt ccgccaatga cgaaattgcc cgtcggattg acatagagct tatactccga
     3781 atcgaacaac ctgacgatcc ggtcaggcat ctcttttaaa gccatcggga taaggatatt
     3841 ccgaatatcc tctttaatct tcgcctgcat aatgtccgga tttttgtcaa attcgtcatg
     3901 ctgcgttgag ataaccaaag tatcgatcct gaccggctgg ttcatttcgt tgtattcgat
     3961 cgtgacctgc gatttggcgt caggccgcag gtaaggcatc aggttcgtct ccttgcggat
     4021 ccgggccagt tgctgcagca ggatatgcgc tacttcgatc ggcatcggca tgaaattgtc
     4081 catctcggta ctggcaaatc cgaacatcat cccctggtcg cctgctcctt gcctgtattt
     4141 ttcctcttcc gttttcgatc ctctctccac gccccggtag atatcgtccg actgcccgtg
     4201 gatcgtcgag atcaccccgc acgaatctgc gtcgaatttg aattctgcct ttgtataacc
     4261 tatctttttt attgtattcc tgactacttc atcgatactg acataccctt tggtgcgcac
     4321 ctcgccggcc gtcacaacca ggcccgtcgt taccagtgtt tcacaggcca ccttcgattc
     4381 gggatcgtga cgtaagaact catccagcag ggcgtctgat atctggtcgg ctaccttgtc
     4441 gggatgacct tccgacaccg attccgaagt aaataaatag ctcattatat cttatttttt
     4501 aattatggaa ttagaggctg tctcaaaagt ctgatatttc gatttgattg cctgaaaaca
     4561 gttcgtgctc caacgactaa agccgttggt tccgaatgtt ttatgagggt tggaaccga
//
LOCUS       DAOPNH010000269         4587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_22004_length_4587_cov_4.660194, whole genome shotgun sequence.
ACCESSION   DAOPNH010000269 DAOPNH010000000
VERSION     DAOPNH010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22004_length_4587_cov_4.660194"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1561
                     /locus_tag="PKZ74_12265"
     CDS             317..1561
                     /locus_tag="PKZ74_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93573.1"
                     /translation="MKKVLYFLLVLVIATSCYKQGQVDETQDKWNGYQKFLKTGEQVH
                     TLWAGRNINVGTVTYGLDDNANFYAKYDCSASGWTISETHMFAGDKLALPRNKPGNPK
                     IGHFPYSGCHNPRVSTYTYYVPLSTLPPCEEPGFVVSAHCVVRSPAGKNETAWAEGDY
                     KFTDKDWGWYDIYFYNQPPNEFTILYATSFTNDTLKLFHVDVTNGNTDLILKETINTG
                     GRIDAAAYDALSGMFFFADYDYNTLYANQMHDTDPSYVSGTITGKAASATFYNNEYYY
                     VDEYTNTINKVSFNTDWTVASETILDTIPSSVLVNDITMNPEGTVIYMIGEVNGGGRE
                     LISWDVATESFYSMAISITTGGQIAYGSDGELYVIAPISEGGSHSLAYTLDPTTGTLT
                     PIEDDIIIIDDPFSDLSKGPIL"
     gene            1816..2676
                     /locus_tag="PKZ74_12270"
     CDS             1816..2676
                     /locus_tag="PKZ74_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93574.1"
                     /translation="MNSFWKYIISFSVSSVLLCGLLGLAIPANEFTDLVKYRLKGKVK
                     SVMEIKYTLDGKGKKATRDRIVYQKFTEFSEEGYESEVTLFRGGSAYLTSRFIPGMNG
                     KPEQMHEYNPDGSVNLTITFLYDEKGYKSAGLYNWSESHNIGEICENSDYYYEIIQND
                     IFTKVVFTNEYRGFCIEENYLKADSTLSFQITAKYDFRGNKLESAYYHGNGRLSWITK
                     YKYDRYDNMIESRIFKSNYIAVLSLYEYQFDPTGNWTTKKEVRDVHINILTEGLEQSD
                     MITERIIEYY"
     gene            2699..4549
                     /locus_tag="PKZ74_12275"
     CDS             2699..4549
                     /locus_tag="PKZ74_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93575.1"
                     /translation="MRKILLFLLSFQVLTLLAQVGVHTDTPDNSSAMDITATNKGLLI
                     PRVTLTSNLSSPSPVTSPAVGLLVFNSGANQPIGFYYWNGSAWTVIGGGGGTGGSDWT
                     LTGNAGTIPGTNFLGTTDNNHFLVYTNNTERMRFESDGQAVFGSTTPNSDSHLFSVIG
                     NTTQNFAVAAYSPTVGFYSFGGRYGMFSQLDSACTPSPGFAMYAKNTDVSGYGMYAVG
                     SNVSSLPLITSHTAAITSYGFDGLHAWGRNTTTGHGICAVGSGQNSSLALTNISAGVS
                     AVGNYGIISRGTAATGRGIVAIGSGQASPSTSSDSEGGAFTGFHGIYARGVNSNGIGV
                     IGVGSNAASYSTVSGGIGGSFAGNYGAYGKGLASDGIGVLGLGSNGSGYYTVTGGSGG
                     AFTGYHGALAVGTDVTMGTGIIAAGNNSSYVIMPAGAGGAFTGVTTGAAGFGTNSSSG
                     IGIIGAGNNQAAWVPSTGCGGAFTGNYGVFCRSVAAAGTGILAAGNNIANPLTYTGGS
                     GGAFTGTEAGVVGYGTTAATGIGVIGVGNNATVSVPSTGCGGAFFGSVCGVYGKANNT
                     DNNNRYGGYFETSGAYAYVGVRYSNTNRKILGTNSVSTIVKNTKGDLVLC"
BASE COUNT         1242 a         1046 c         1091 g         1208 t
ORIGIN      
        1 aagctgtcgg ttcccgcatc acatttatta cggagcttat ttgcagcgaa ttatttatat
       61 tcgttacaaa taagcttttt attttcacat tacacaattt ccacgtgtaa cctttataat
      121 agtaacggga ttaatgtttc ctgggtctgt tttttttctg ctatttcccc gccatcccga
      181 aaaaaataaa accttattga aacctgcccg tataaccagg tcggtatcgg taacgattat
      241 cattttttgt cctttgaatt gaattgaatt gaaaacaaac taaatcatcg aaaattaact
      301 ttttaaaaac tgaattatga aaaaagtact ctatttcttg ttggtactgg tcatagcaac
      361 ctcctgttac aaacaaggac aggttgacga aacccaggac aaatggaacg gttaccagaa
      421 attccttaaa acgggcgaac aggtccatac attatgggcc ggcaggaaca ttaatgtcgg
      481 gactgtaact tatggcctcg atgacaatgc aaacttctat gcaaaatatg actgcagcgc
      541 ttccgggtgg acgatctcgg aaacccatat gtttgccggt gataaactcg ctttaccgcg
      601 aaataaaccg ggtaatccta agattggcca tttcccctat tccggttgcc ataatccaag
      661 ggtcagcacc tatacctatt atgttccgct gagcacgctt cctccatgcg aagagcccgg
      721 atttgtcgtt tcggcccatt gcgtggtgcg cagccctgcc gggaaaaatg aaactgcctg
      781 ggcagaaggt gattacaaat ttacagataa agactgggga tggtatgata tctattttta
      841 caatcagcct ccgaatgaat tcactatttt atacgccact tccttcacga atgatacctt
      901 gaagctgttc catgtggatg tgactaacgg aaatacggat ctgatcctga aggaaacgat
      961 caataccggc ggccggatcg atgcagcagc atatgatgca ttgtcgggca tgttcttctt
     1021 tgccgactat gattataaca ccctgtatgc caaccagatg catgataccg atccttccta
     1081 cgtttccgga acgattaccg gtaaagccgc cagcgctacc ttctacaaca atgaatatta
     1141 ttatgtcgat gagtacacca atacgatcaa taaggtctct ttcaataccg attggactgt
     1201 tgcctctgaa actatcctgg atacgatccc gagttcggtc ctggttaatg atatcactat
     1261 gaatccggaa ggtaccgtga tatacatgat tggtgaagtg aacggtggag gaagagaact
     1321 gatctcctgg gatgtcgcta ccgaatcgtt ctattctatg gcaatcagta ttactacggg
     1381 cgggcagatc gcttacggaa gcgacggtga attgtatgtg atcgccccga tatcggaagg
     1441 aggaagccat tccctggcct atactctgga tccgaccacc gggactttga caccgattga
     1501 agatgatatt atcattattg atgatccgtt ctcggatctt tccaaagggc caatccttta
     1561 aataaaatag atgaatgaga ggctgtcatg atttacctgg cagcctcttt ttttgtcctc
     1621 atggaaagta acagtatcga tcaggcgctt ttttaactaa ttttcatcaa cacggccatg
     1681 tggataaatt gtcgctaaag cccagtgtat taaatgatat ctatattact tttttacttt
     1741 ttttcgataa acctgagcat gttgagcaat gcattcgaat tatatgcttt aaaccactaa
     1801 attgatcatc tcccggtgaa ttccttttgg aaatatatta tttctttttc tgtatcttct
     1861 gttctccttt gcggattgct cggcctggcc attcctgcaa atgaatttac tgacctggtg
     1921 aaataccggc ttaaagggaa agtcaaatcg gtcatggaaa tcaaatatac attggatgga
     1981 aaaggaaaga aagccacccg tgacaggatc gtttaccaga aattcaccga attcagcgag
     2041 gagggttatg aatcggaggt cacccttttc agaggcggat cggcctacct gacttccagg
     2101 tttattccgg gcatgaacgg aaaacctgaa cagatgcatg aatacaatcc cgacggttcg
     2161 gttaatttaa ccataacatt tttatacgat gaaaaaggat ataaatctgc aggcctttac
     2221 aattggtccg aaagtcacaa catcggtgaa atctgtgaga actctgacta ttattatgaa
     2281 atcattcaaa acgatatttt cacgaaagtg gtgtttacaa atgaatacag gggcttttgt
     2341 atcgaagaaa attacctgaa agcagacagt accctctctt tccagatcac tgccaaatac
     2401 gacttccggg gaaataagct cgaatcggcg tattaccatg gtaacggacg cttatcgtgg
     2461 atcaccaaat acaaatatga ccgctatgat aacatgattg aaagccgaat ctttaaaagc
     2521 aattatatcg ccgttctatc cctgtatgaa taccagttcg acccgacagg caactggacc
     2581 acgaaaaaag aggtacggga tgtccatata aatattctga ctgaaggtct ggaacagtcg
     2641 gatatgataa ccgaacgcat catcgaatat tactaacccg cttgaaaata tcagggctat
     2701 gagaaagata ttactttttt tgctctcctt ccaggttctg acactcctgg cgcaggtggg
     2761 ggtgcatacc gatactcccg acaactcctc tgcaatggat atcactgcaa cgaataaagg
     2821 gctgctgatc cccagggtta cccttaccag caatttgtca agcccttctc cggtaacatc
     2881 gcccgctgtg ggccttctcg tttttaattc aggcgctaac cagccaatag gtttttacta
     2941 ttggaacgga tcggcatgga cagtcatcgg cggaggtgga ggcacgggcg gtagtgactg
     3001 gaccctgacc ggtaatgcgg ggacgattcc cggcacaaat ttcctcggga caacggacaa
     3061 caatcatttc ctggtataca ccaacaacac ggaacggatg cgcttcgaat ccgatgggca
     3121 agcggtcttt ggcagcacaa ctcccaactc cgattcccac cttttttcgg tcatcggtaa
     3181 cactacccag aattttgcgg tggcagcgta ttcgccgaca gtcggttttt attcctttgg
     3241 cggaagatat ggaatgttct cccagctcga ttcggcctgc acccccagcc cgggatttgc
     3301 catgtatgca aaaaatacag acgtttccgg ttatggcatg tatgcggttg gcagcaacgt
     3361 ctcttcactc ccgttgataa ccagccacac ggcagctatt acaagctacg ggtttgatgg
     3421 cctgcacgcc tggggaagga atacaacgac cgggcatgga atatgtgccg ttggcagcgg
     3481 acagaacagc tccttagcat tgaccaatat atctgcaggg gtctcagccg ttggcaatta
     3541 tggaataatc tccagaggaa cggcggctac cggcaggggc attgtggcca tcggcagcgg
     3601 tcaggcctcc ccgtcgactt ccagcgacag cgaaggaggt gcctttacag gattccacgg
     3661 tatctatgcc cgtggcgtga acagcaacgg aatcggcgtg atcggcgtgg gaagcaacgc
     3721 agcatcctat tcgacggtat caggcgggat cgggggcagt tttgcaggaa attatggcgc
     3781 ttacggaaaa ggcctggctt ccgatggcat cggtgttctc ggcctgggca gcaatggttc
     3841 gggatattat accgttacag gaggaagcgg aggcgctttt accgggtatc atggggcgct
     3901 ggccgtcgga accgatgtaa ccatggggac aggcatcatt gccgccggga acaacagcag
     3961 ttacgtgatc atgccggccg gcgccggggg agcgtttaca ggcgttacca caggagccgc
     4021 cggttttgga acaaattcca gcagtggcat aggtattatc ggtgctggaa ataaccaggc
     4081 agcctgggta ccgtccaccg gatgcggcgg ggccttcacc ggtaattacg gcgtcttctg
     4141 ccgctcggtt gctgcagcag gaacagggat attagctgcc ggtaacaaca tcgccaatcc
     4201 gctgacatat accgggggca gcggcggcgc tttcaccggg acagaagccg gtgtcgtcgg
     4261 ttacggaaca actgcagcta ccgggatcgg cgttatcgga gtgggtaata atgctacggt
     4321 aagtgttcct tccaccggtt gtggaggcgc tttctttggc agcgtttgtg gagtttatgg
     4381 aaaagctaat aatactgata ataataaccg atatggggga tattttgaaa cttccggagc
     4441 ctatgcttat gtaggagtca ggtacagcaa tacaaacaga aaaatattgg gcactaactc
     4501 tgtaagtact atagttaaaa acactaaagg tgatctagtg ttatgttaaa catgaattga
     4561 cggatataat cgtttaagtt ttattct
//
LOCUS       DAOPNH010000270         4525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_22405_length_4525_cov_5.892170, whole genome shotgun sequence.
ACCESSION   DAOPNH010000270 DAOPNH010000000
VERSION     DAOPNH010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22405_length_4525_cov_5.892170"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKZ74_12280"
     CDS             complement(<1..1253)
                     /locus_tag="PKZ74_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM93576.1"
                     /translation="MRRKILVLTVLMHFMLSGANAGKIVHVYEFNRPEISYSGPYGIV
                     YFEGTKLAGQTGKATLPFYPVKILLPPGEKAESISISYKDPVVLQEKIMLMPFQEVRP
                     LSDPGESLMAFDETFYQSTTPYPSYFKPHLETQFFNGCGIALSSFTPISYLPKENQIT
                     YYQRVEIEIITLTDPAQSHNKHFFGSEQKSLWLKDLVQNPEKISDYLSGREFLTSEYD
                     ILVITKNQYLGEFDTLKSFYELQGLKTKVAATETIGSSMSGIDLQQKIRNFIIQEYDN
                     NGISYVVLGGDTEIVPYRGFYCYVQSGGGYSSTNIPADVYYSALDGSWNNDGDARWGE
                     PDEDDLYPEVAIGRITFSDTAELHNILHKSFLYQCDPVEGELTKPLLAGEELYDDPYT
                     IGSDYLDLLIGYRTDNGYSTHGIPPS"
     gene            complement(1267..1971)
                     /locus_tag="PKZ74_12285"
     CDS             complement(1267..1971)
                     /locus_tag="PKZ74_12285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM93577.1"
                     /translation="MNIVVTGTSRGIGYETVKLFAAAGHNKILAVSRHKARLEKLRSD
                     CEKENETSKIFIVDVDIAGDNFESRVVREVRENLGTVDILLNNAGALVNEPLDRLTKG
                     DFDLMFGVNVKSAFLMIQNLLPYFNPGAHIVNISSMGGVQGSVKFNGLSLYSASKGAL
                     AVLTECLALELKDSGISVNCLALGAAQTEMLAEAFPGYQAPLGATEMAGFIADFSMNG
                     HKWFNGKILPVALSTP"
     gene            2075..2479
                     /locus_tag="PKZ74_12290"
     CDS             2075..2479
                     /locus_tag="PKZ74_12290"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPM93578.1"
                     /translation="MRAILLPLILFVLAQSLPASLKAQDEPFDDISKAIRESDARSLA
                     AFFSATIEISLPDAENTYSASQGEMVMKDFFNRIPADNLTLVQKGSLNTSSHYSISTY
                     HSGGKNYQLYMEIRKEKEKFRLFKIKLEEKIK"
     gene            2490..3332
                     /gene="nadC"
                     /locus_tag="PKZ74_12295"
     CDS             2490..3332
                     /gene="nadC"
                     /locus_tag="PKZ74_12295"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPM93579.1"
                     /translation="MTIEEIIRKALVEDLGGGDHTSLATIPVGATGRSRLLIKQEGIL
                     AGIDVAREVFRQVDPRLAFEKLLDDGAAIRPGDVAFYLSGPGISMLSAERLALNFMQR
                     MSGIATATHNLVKLIEGTRAKILDTRKTTPLLRELEKMAVRAGGGFNHRMGLYDMIMI
                     KDNHVDFAGGISRAIEAVHQYMSQLKNGNDLKIEIEVRNFRELDEVLQKGGIDRIMLD
                     NFTVEDLARAVSIIGGKYETEASGGITAATIRSYAETGDDFISVGALTHHINSLDMSL
                     KAVI"
     gene            3334..4308
                     /locus_tag="PKZ74_12300"
     CDS             3334..4308
                     /locus_tag="PKZ74_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPM93580.1"
                     /translation="MIKSPISNITENVKSLATKLFKLVLLPLIILSRNIYLPGFKGVP
                     LYDVGLFFVRGMQKSSISIRANSISYSFIIAFVPAVLFLFTLIPYIPVAGLKENLLMT
                     LEEVIPREAFVVLRSTIEDIITNQQSGLLSISFLISLYFASNGVTAIMNAFNQSSHSI
                     ESRTLFKRQLVALLLVFILALNMILSAAALAFNSVFLYFMEKQGFISNNITLLLLEAG
                     QWVIIFLTSLMAFSSIYYMAPAKKRIFPFFSAGSILASFLSVIAFKIFTVFIENFTDY
                     NRFYGSLGSMIMIIVWINLTALMLLIGFELNASIYEAKKQGEKKNTGQ"
BASE COUNT         1156 a         1107 c         1050 g         1212 t
ORIGIN      
        1 gatggcggga tgccgtgcgt gctatagccg ttatctgtgc ggtaaccgat cagcaggtcg
       61 aggtaatccg acccgatcgt ataaggatca tcatacagct cttcgccggc cagaagaggc
      121 tttgtcagct caccctccac cggatcacac tggtaaagaa agcttttgtg caggatgttg
      181 tgaagttcgg ccgtatcgct gaaagtaatt ctcccgatgg caacttcggg gtagaggtca
      241 tcctcatccg gctcgcccca gcgcgcatcg ccgtcgttgt tccacgaacc gtcaagtgca
      301 gaataatata catcggccgg aatgtttgtg ctggaatagc cgccccccga ttgcacataa
      361 cagtaaaatc cgcgataagg aactatctcc gtatctcctc caagaacaac ataactgatg
      421 ccgttgttgt catactcctg gataataaaa ttcctgatct tctgctgcag gtcgatgccg
      481 ctcatcgaag agccaatggt ctcagtagcc gccactttgg ttttcagtcc ctgtaattca
      541 taaaaggatt tcaacgtatc gaattcgccc aggtattgat tctttgtaat aaccaggata
      601 tcatattcac tcgtcaagaa ctctcttccg gacaaataat cgctgatctt ttccggattt
      661 tgaacgaggt ctttcagcca gagtgatttt tgctctgatc cgaaaaagtg cttgttatgg
      721 ctttgcgcag ggtcggtaag ggtgatgatc tctatttcaa cacgttgata ataagttatc
      781 tgattttctt tcgggagata agaaattggg gtaaaagaag acaaggcgat tccgcagccg
      841 ttgaaaaatt gagtttccag gtggggctta aaataggatg gatagggtgt ggtgctctgg
      901 taaaaggttt catcgaaagc catcagtgat tcgcccggat cagacagagg acgcacttcc
      961 tggaaaggca tcagcatgat cttctcttgc aggacgacag gatctttgta cgaaatcgat
     1021 atggattcag ccttctcgcc gggcggcaaa aggatcttca ccgggtaaaa cggcagtgtc
     1081 gctttgcccg tttgcccggc cagtttcgtt ccttcaaaat atacgatccc gtatggtccg
     1141 gaataactga tctcagggcg gttaaattca tatacgtgaa caatctttcc ggcgttagct
     1201 ccgctaagca tgaagtgcat cagaaccgtt aaaaccagta tttttcgtct cattggaata
     1261 ttcaggttac ggagtcgaca gtgcgacagg taaaatcttc ccgttaaacc acttatgacc
     1321 attcatcgaa aagtcggcga taaagccggc catttcggtt gcacccagag gagcctgata
     1381 accggggaaa gcttcggcca gcatctctgt ctgtgccgct cccagtgcca ggcaattcac
     1441 gctgatcccg ctgtctttca gttccagggc caaacattct gtcagcacgg ccagcgcccc
     1501 tttgctggca ctatagagcg acaggccatt gaacttgacg ctaccctgaa ccccacccat
     1561 actactgata ttgacaatgt gcgcaccggg gttgaagtaa ggtaaaagat tctgtatcat
     1621 caggaaagcg ctttttacat ttacaccgaa catgaggtcg aaatcccctt ttgtcaatct
     1681 gtccaggggc tcattcacca gcgcgccggc attgtttagc aggatatcca ccgtaccgag
     1741 attttccctg acttcgcgaa ctacccggga ttcgaaattg tcgccagcaa tatcaacatc
     1801 aacaatgaat attttactgg tttcattctc cttttcgcaa tccgaccgca gcttctcaag
     1861 tcgagcctta tgacgtgaaa ccgccagtat cttattatgc ccggcagcgg cgaaaagttt
     1921 taccgtttcg taacctattc cccggcttgt tcccgtgaca acaatattca tatattcgat
     1981 attatattac aaaaatagta aaaacgctct gttcatcgca acgaatatta cacctgacga
     2041 tacaattatt gtaattttgc atgaaaatcc gggtatgaga gcaatacttc ttccgttgat
     2101 tctgtttgtc ctggcgcagt ccttaccggc ctcattaaag gcacaggacg aaccgttcga
     2161 cgatatcagc aaagctatcc gcgaatcgga cgcacgaagc ctggcagcat ttttcagtgc
     2221 tacgatagag atcagccttc ccgatgctga aaacacttac agcgcctcgc agggcgagat
     2281 ggtgatgaaa gactttttca acaggatccc ggccgataat ctcactcttg tccagaaagg
     2341 ttcgctcaac acctcttctc attattcgat ttctacatat cactcgggag gtaagaatta
     2401 ccagctttat atggaaatcc ggaaagagaa ggaaaagttc cggttgttca aaattaaact
     2461 ggaagaaaaa ataaaataat tgcgctaaga tgacgatcga agaaatcatc cggaaagcgt
     2521 tggtagaaga cctgggcggc ggggatcaca cctcgctggc aacgattccc gtcggcgcaa
     2581 cggggcggtc aaggctgctg atcaagcagg aagggatcct ggcaggcatt gacgtggcca
     2641 gggaagtttt ccggcaggtc gatccccgtc ttgcctttga aaaactactg gacgatggag
     2701 cagcgatccg gccgggtgat gtggcctttt acctgtccgg gccaggcatc tcgatgcttt
     2761 cagcagaacg gctggccctg aacttcatgc agcggatgag cggcattgcc acggccacgc
     2821 acaacctggt gaagctaatt gagggtaccc gcgcaaagat cctggatacc cggaagacta
     2881 ctcccctgct ccgtgaactg gaaaaaatgg ccgtgcgggc cggtggagga ttcaatcaca
     2941 ggatgggcct gtacgacatg atcatgatca aagacaatca tgtcgacttt gccggcggta
     3001 tcagccgggc tattgaggcc gtacatcaat acatgagcca actgaaaaat ggaaatgatc
     3061 tcaaaattga gatcgaggta aggaatttcc gggaactgga tgaagttttg cagaaagggg
     3121 gcatcgaccg tatcatgctc gataatttta cagtcgaaga tctcgcccgt gccgtcagca
     3181 tcattggagg caaatacgaa acagaggctt cgggtggaat tactgcggcc accatccggt
     3241 cgtatgcaga aacaggcgat gatttcatct ccgtcggagc gctcacacat catatcaaca
     3301 gcctggatat gagccttaaa gcggtaatct gacatgatca agtcgccgat cagcaacatc
     3361 accgaaaatg taaaaagcct ggccacaaag ctatttaagc tggtgctgct ccccctgatc
     3421 attctttcaa gaaatattta tcttccgggc tttaagggtg tcccgcttta cgatgtggga
     3481 ttgtttttcg tccgtggcat gcagaaaagc tccatctcta tccgggccaa ttccatctcc
     3541 tattccttta tcatcgcctt tgtcccggca gtcttattcc tgttcaccct cattccttac
     3601 attcctgtcg ccggattgaa agaaaacctg ttgatgacgc tcgaagaggt gatcccccgg
     3661 gaagcattcg ttgttctccg gtcaaccatc gaagacatca ttaccaacca gcaatcgggc
     3721 cttttatcca tcagctttct catctcgctc tactttgcca gcaacggcgt tacggctatc
     3781 atgaacgctt ttaaccagtc gtcgcattcg atcgaatcgc gcacactttt caaaaggcaa
     3841 ctggtggctt tattgctggt ttttatcctg gccctgaaca tgatcctgtc ggcggccgcg
     3901 ctggcattta attcggtttt tctttatttt atggaaaaac agggctttat cagtaacaat
     3961 ataacgttgc ttctcctgga agcaggccag tgggtcatta ttttcctgac ctcgctcatg
     4021 gcattttcca gcatctacta tatggcaccg gccaagaaaa gaatctttcc cttcttctcc
     4081 gccggatcga tcctcgcctc gttcctttcg gtcatcgctt ttaaaatatt tacagttttt
     4141 attgagaatt tcaccgatta taaccggttc tacggttcat tggggtcgat gatcatgatc
     4201 attgtctgga taaacctgac tgcgttgatg ctgctgatcg gatttgaact gaatgccagc
     4261 atttatgagg cgaagaaaca gggggagaag aaaaataccg gacaatgaat ttaaatttat
     4321 tgaattcttt tctttaaatt tactccatat tcgttatcaa tgaatctggt gatccaatgt
     4381 agccgattat tcaggcgtta tctattcaga ttctgtatta catttttatc tttcattgta
     4441 ccttgttcaa taatctttgg tcaatcggga acagggatgc cggtctggtg catttcacct
     4501 gcaagtgaag cgggtgtcct tgtta
//
LOCUS       DAOPNH010000271         4494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_22625_length_4494_cov_6.562064, whole genome shotgun sequence.
ACCESSION   DAOPNH010000271 DAOPNH010000000
VERSION     DAOPNH010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22625_length_4494_cov_6.562064"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="PKZ74_12305"
     CDS             complement(<1..1932)
                     /locus_tag="PKZ74_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93581.1"
                     /translation="MIRITDKAKLREIVTGTLLGLLVAIVTFPAPDWSYGPGIDPPLA
                     WVFNSLFLNGSGSDPVIIFPHGPLAFFTYPTAENAWLVMIVTIFLKIMLILSIKQFIT
                     AGKTWTKWLAASAVTYLVSVSGGFSHLLLANIMAGYAAGFHQGFSKWTARYFLVLLLT
                     AIAFYVKAYTAIIAGILFISSGIYHLIKTKKVLPFLTDSFILLIIILLFWLFLFRSFH
                     GFITYVNGMVHLAQDNSSAAAYYPYNNWLVLSSFILLIIIIIIVNRNKESLFFSSLMG
                     LSLFAAWKHGMAREDIYHVMGFFVFVMMSLALFILFSGKRLIINILIATAALFLLSLN
                     SKNAMNYSPRKYEWFSAGNFFSFISDHKTLKDDAQTATSRAIAINILPDYLLEFINDY
                     TTDVYPWDYSIIPANNLNWQHRPVIHPYASYTPWLDRQNAGHFSSAMAPDYIVWEKDK
                     ITSDVNGGKFNSIDGRYLLNDEPQTLLELINRYDFVAEEGKFILLGKRETSLQPVIHT
                     ISTVQGHFREWFPVPDTRGDILRVKLNFKKTFLQRLKSFLYKDEQFWIYLKLTNGEIH
                     KYRIVPANANVGIWIKPYLFNYDKEFEVDEIMFVASNPGILTENLSLEWEMIDFDDDG
                     NRAVSFFNIDDDREDSLIFV"
     gene            complement(1963..3474)
                     /locus_tag="PKZ74_12310"
     CDS             complement(1963..3474)
                     /locus_tag="PKZ74_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPM93582.1"
                     /translation="MKILPVEKIREADAYTIAHEPVESVDLMERAAMKCYNWLKKRVD
                     ADRPVKIFCGPGNNGGDGLAIARMMAESHHQVSVYVIRYSEKSSRDFLINYERLTGHS
                     SVPVFDIKDGDRLPEISEEDVVVDALFGSGLNKPAEGFSAAVIRHINASRSLIVAIDM
                     PSGLFCDMPTDEKAGPVVRADYTLTFQAPKLAFLFAENDIRVGEWVVLDIGLHPGFTD
                     SLEVKNFLLTREMASGLLKPRTKFAHKGHFGHALLIAGGYGRMGAAVLAAEACLRSGV
                     GLLHAHAPAKGVNVIQTAVPEAMITIDADEECLSSLPDLSPFTAIGIGPGIGFDMKTK
                     NALKLLIQNTSVPMLFDADALTILAENRTWISFVPRNSVFTPHPKEFERLAGKATDDF
                     HRNRLQREFCIRHGVWIVLKGAHSCICGPDGVCYFNTTGNPGMATGGSGDVLTGLILG
                     LLAQRYSPRDACLLGVFLHGLAGDLAAKKIGMASLLAGDITRNIGKAFKKLSS"
     gene            3599..>4494
                     /locus_tag="PKZ74_12315"
     CDS             3599..>4494
                     /locus_tag="PKZ74_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPM93583.1"
                     /translation="MSRRSKNRQPLPLLEKVEIIDAGAEGKAVARVENRVVFIPFGVP
                     GDIVDVQVTGRKKSFFEGRIVKFHVMSSSRVEPACVHFGLCGGCRWQNMDYDRQLHFK
                     QKQVQDNFDRIGHFKYPEIRPILGSDNIYFYRNKLEYTFSNRKWFTGARPEAGSNENT
                     NGLGFHLPGMFDRILDIEKCHLQADPSNEIRQAAREYAFENNLSFYDVKTWQGLMRNL
                     VIRTTQAGEVMVIVVFHDKEDEVIRGFLSFLERRFPEITSLLYVINGKHNDDISDQEI
                     RLFAGKPWLEEILKAFHPGDPPL"
BASE COUNT         1178 a         1107 c         1137 g         1072 t
ORIGIN      
        1 aacgaaaatc aaactatcct cccgatcatc gtctatatta aaaaaactca cagccctgtt
       61 cccatcatca tcaaaatcaa tcatttccca ttcaagagaa agattttcgg tcaatattcc
      121 gggatttgag gcgacgaaca tgatctcatc cacctcaaac tctttatcat aattaaataa
      181 atacggtttt atccaaatcc caacattggc gtttgcagga acgatgcggt atttatgaat
      241 ctccccgttg gtaagtttca ggtaaatcca gaattgttca tctttataaa gaaagctttt
      301 cagtcgttgc aggaacgtct tcttaaaatt gagcttcacc cggaggatat cgcccctggt
      361 atcaggcacc gggaaccact ccctaaaatg cccctgaaca gttgaaatcg tatgaattac
      421 aggctgcaag gatgtttcac gcttacccag cagaatgaat ttcccctcct cagccacaaa
      481 atcataacga ttgatcagtt ccaggagagt ctgtggttca tcattgagaa ggtatcgccc
      541 gtcgatacta ttaaatttgc cgccattaac atcactggtg attttatcct tttcccagac
      601 gatgtaatcg ggggccatgg ctgaactgaa atgcccggcg ttttgccggt cgagccaggg
      661 cgtgtacgaa gcataggggt ggatcaccgg ccggtgctgc cagttcaggt tattggccgg
      721 aataatggaa taatcccagg gataaacatc tgtcgtataa tcatttataa attccaggag
      781 ataatccgga agaatattta ttgcaattgc cctggaagta gcggtttgcg catcatcttt
      841 taaagtttta tgatccgaaa taaaactgaa gaaattcccg gcactgaacc attcgtattt
      901 ccggggagaa taattcatgg catttttact attgagcgac aaaagaaaca gggcggctgt
      961 tgcaattaaa atattaataa taagtcgttt acctgaaaaa aggatgaaca gggccaggct
     1021 catcattaca aatacgaaga atcccatcac atgataaata tcttccctgg ccattccatg
     1081 tttccaggca gcgaagaggc ttaatcccat cagggagcta aagaagagcg attctttatt
     1141 ccggtttacg attataataa tgataatcaa taatatgaat gaggacaaaa caagccagtt
     1201 gttgtatgga taatacgctg ccgcggatga gttgtcctgt gccaggtgga ccatgccgtt
     1261 cacataggtg atgaatccat ggaagctccg aaacagaaat aaccagaata aaagtatgat
     1321 aatgagcaat ataaagctat cggtcaaaaa aggtaatacc ttttttgttt taatgagatg
     1381 ataaattccg gaagaaataa acaggatccc ggcaatgata gccgtgtagg ctttaacgta
     1441 gaaagctatc gctgtaagca gcagcaccag gaagtatctc gcggtccatt tcgaaaaccc
     1501 ttgatgaaat ccggcggcgt aaccggccat gatattcgcc aggagcaggt gggagaatcc
     1561 gccgctcacg gagaccaggt aagtgacagc cgatgcagcc agccacttcg tccaggtctt
     1621 tccggcagta ataaattgct tgatgcttag aatcagcatt atcttgagaa atatcgtcac
     1681 gatcatcacc agccaggcat tttcagccgt cggataggta aagaaagcca aaggtccgtg
     1741 ggggaagatg attaccggat cgctcccact cccgttcaag aacagggagt taaacaccca
     1801 ggccagcgga gggtcaatgc ccgggccgta tgaccagtcg ggcgccggaa aagtgacgat
     1861 ggctaccagc aatcccagca gggtcccggt gactatttct ctcagcttcg ctttgtcggt
     1921 tatccggatc attcagaatg gtggtaatta agatatttaa tatcaggaag ataacttttt
     1981 aaacgctttc ccgatattcc tggtgatatc gccggccagc agcgacgcca tcccgatttt
     2041 cttcgcagcc aggtctccgg ccaggccatg gaggaaaacg cccagcaggc aggcatcgcg
     2101 cggactatag cgctgcgcca gcagtcccag gatcagcccg gtgagcacgt cgccgctgcc
     2161 gccggttgcc atccccgggt tgccggtagt gttgaaatag caaactccgt ccggaccgca
     2221 gatgcaggaa tgggctcctt tcagcacgat ccagacgccg tgccggatac agaattcgcg
     2281 ctgcagccgg ttccggtgga aatcatcggt ggcttttccc gccagccttt caaattcttt
     2341 cggatggggt gtgaagacgc tgtttcgggg cacgaacgag atccatgtcc ggttctcggc
     2401 caggatcgtc agcgcgtcgg catcaaaaag cattggaacg gacgtgttct gtatcaggag
     2461 tttaagtgca tttttggtct tcatatcaaa accgatgccc gggccgatgc caatggcagt
     2521 gaagggcgac agatcgggca atgatgagag gcactcctcg tcagcgtcga tcgtgatcat
     2581 cgcttcggga actgctgtct ggatcacgtt cacccccttt gccggggcat gggcatgcag
     2641 caagccgacg cccgagcgga ggcaggcctc tgcggccaga acggcggccc ccatccggcc
     2701 gtagccgccg gcaatgagca acgcatggcc aaaatgccct ttgtgggcaa atttggtgcg
     2761 gggttttagc agtcccgagg ccatttcgcg agtcagcagg aaatttttaa cctccaggct
     2821 gtcggtaaaa cccgggtgaa ggccgatgtc gagcaccacc cactcgccta ccctgatatc
     2881 gttttcggcg aacaggaagg caagcttggg cgcctggaaa gtaagggtat agtcggccct
     2941 gacgaccggc ccggctttct catccgtggg catgtcgcag aacaggcccg acggcatgtc
     3001 gatggcaacg atcagcgacc ggctggcatt gatatgccgt atgactgcgg cggaaaaacc
     3061 ctccgccggt ttattcagcc ccgaaccgaa aagagcatcg accactacat cctcttcact
     3121 gatttccgga agacggtctc catcttttat atcaaaaaca ggaacactgc tgtggccggt
     3181 cagccgttcg tagttgatca gaaaatcccg ggacgacttt tccgaatagc ggatcacata
     3241 aaccgaaacc tggtgatggc tttcggccat catccgggcg atcgccagcc cgtcgccgcc
     3301 gttgttcccc ggtccgcaga atatcttcac cggccggtct gcatccactc tctttttcag
     3361 ccagttgtag catttcatgg cggcccgttc catcagatcg accgattcga caggctcatg
     3421 agcgatggtg taggcatccg cttcgcgtat cttttcgacg ggcaggattt tcatggcata
     3481 tctggtattt catacaaagg tagtgtttga cctgttacaa taattgatcc gctacgcgga
     3541 aattgaattt cacttcaggg ataaaccaat cggcaataaa cattattttt accgtgaaat
     3601 gagcagaaga tcgaaaaaca ggcaaccgtt gcctttactg gagaaggtgg agatcatcga
     3661 cgcaggcgct gaaggcaaag ccgtggcacg tgtcgaaaac cgcgtggtat tcatcccgtt
     3721 cggagtcccc ggtgatattg tggatgtgca ggttaccggg aggaagaaat cgttctttga
     3781 agggagaatc gtcaaatttc atgtgatgtc ttcatcgcgg gttgagccgg cttgcgtcca
     3841 ttttggcctg tgtggcggat gccggtggca gaacatggat tacgaccggc agcttcactt
     3901 caagcaaaaa caggtgcaag acaatttcga ccggatcgga cattttaaat acccggaaat
     3961 caggccgatc cttggcagcg ataatattta tttctaccgg aacaagctcg aatacacatt
     4021 ctcaaaccgc aaatggttca ccggggcacg gccggaagct ggaagcaatg aaaacaccaa
     4081 cggactgggt tttcacctgc ccggcatgtt cgaccggatc ctggatattg aaaaatgtca
     4141 tttgcaggcc gatccatcca atgagatccg ccaggcagcc agggagtatg cgtttgagaa
     4201 caatctcagt ttttacgatg ttaaaacctg gcagggcttg atgcgtaacc tggttatccg
     4261 cacgacgcag gccggcgagg tgatggtcat tgtggttttt catgataagg aggatgaggt
     4321 catacggggt tttctatcgt ttcttgagag gcggtttccg gaaatcacat cacttctgta
     4381 tgttataaac ggcaagcaca acgatgatat ctccgaccag gagatcaggt tgtttgcagg
     4441 aaagccctgg ctggaagaga tcctgaaagc ctttcacccc ggcgatcctc cgct
//
LOCUS       DAOPNH010000272         4481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_22727_length_4481_cov_6.406236, whole genome shotgun sequence.
ACCESSION   DAOPNH010000272 DAOPNH010000000
VERSION     DAOPNH010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22727_length_4481_cov_6.406236"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKZ74_12320"
     CDS             complement(<1..540)
                     /locus_tag="PKZ74_12320"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPM93584.1"
                     /translation="MRFTIEATDIQSNARAGILETGHGLIHTPIFMPVGTAGTVKALH
                     FKDLKDVVRAEIILGNTYHLYTRPGMPVIESAGGLHRFNGWEGPILTDSGGYQVYSLA
                     DSRKLTENGVTFQSHIDGSRHTFTPESVIDIQRSIGADIIMAFDECPPAQAPRQYVEE
                     SMHLTHRWLNRCMGRFIETS"
     gene            606..1748
                     /locus_tag="PKZ74_12325"
     CDS             606..1748
                     /locus_tag="PKZ74_12325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM93585.1"
                     /translation="MIEFPFKPSVHEILPFAVFILFGITWLIQMVYYWGIFSRLAFFR
                     RKATTADTPPVSVIICAKNEYRNLIQNLPLILEQDYPDYEVIVVNDASDDDTIELLSS
                     FTRKYSHLRIFNLEQNLNFFSGKKFPLSLGIKSARHEIVLLTDADCQPSGKEWIRKMM
                     ENYGPGTEIVLGYGTFRKTAGFLNQIIRYDAFLVAVQYLSFALSGSPYMGVGRNLSYK
                     KNLFFKNGGFISHYKLKSGDDDLFINRVARRRNTRIEIHPDAHTISSPKSGFYQWILQ
                     KRRHYTTARFYRPAFKFLLSLGYVSKLLLYLSFIALMVVRYNWMIVLSAFSLFFISHW
                     IILSFAANKMKENDLAVLSPIYEVLLLIISPLLYISNIVHKQERWK"
     gene            1739..2359
                     /locus_tag="PKZ74_12330"
     CDS             1739..2359
                     /locus_tag="PKZ74_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM93586.1"
                     /translation="MEISQRMTGKAIRDFDLVKAALEKGDQNAYAELMKNYRDALYFM
                     MLKMTNNPHDAEDLTIEAFGKAFRNLRQYTPDYAFSTWLFKIASNNCIDFIRKKKLNE
                     NISFNLNDLPDSGDGLADILPTTSRNPEEDIIRKQKIETLRLVIDKLKPNYRRLIELR
                     YYEEKSYEEISEALDLPIGTVKAQLFRAREFLYHFLSNRHDKLYEE"
     gene            2443..3318
                     /locus_tag="PKZ74_12335"
     CDS             2443..3318
                     /locus_tag="PKZ74_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93587.1"
                     /translation="MKRLKILIIILMGALMITSCGKKKEQQILDLQAKNAELLAESNR
                     KDSLMNELFTSINDIERNLADVTAREKLITDAPVSEQRMNEDVRTKIMDEIILINNIM
                     EENKKQIAALKDQLKKSGAKVAALQETINLLTQRLEEKEMEIADLKNQLVRQNFRIEE
                     LNATVDTLQEEKASQSRIISQQDALIGEMNTIWYVSGPKKELLEKGIIEKAGGMLSGK
                     MKMSDLINPGYFVTADMRKISVIPFEGDKAEMVTVHPEGSYKFLMEGDTFKGIEILNP
                     GEFWKSSRFLVIMTK"
     gene            3321..>4481
                     /locus_tag="PKZ74_12340"
     CDS             3321..>4481
                     /locus_tag="PKZ74_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPM93588.1"
                     /translation="MKLIDFQKAIATGIGDVLPEPKPYDRSVSHAPRRKDILTPEEKR
                     LALRNALRYFKPEHHPVLASEFATELERFGRIYMYRFRPDYPMFARPIGEYPHRSLQA
                     AAIMLMIQNNLDPAVAQHPHELITYGGNGAVFQNWAQYLLTMRYLSEMTNEQTLVMYS
                     GHPLGLFPSHPDAPRVVVTNGMVIPNYSSQDDYERFNALGVSQYGQMTAGSYMYIGPQ
                     GIVHGTTITILNAGRRLDPGNGSLAGKVFVTSGLGGMSGAQAKAAVIAGAVGVVAEVN
                     PAAVSKRHEQGWVDEVFTDLDLLLARIQKARTLREAVSIAYQGNIVDLWEKMAEQQFD
                     VELGSDQTSLHNPWAGGYYPAGITFEESNRLMAEDPARFRELVKGSLIRHVKA"
BASE COUNT         1242 a         1022 c         1075 g         1142 t
ORIGIN      
        1 tgacgtttcg ataaacctgc ccatgcagcg gtttagccac cggtgggtaa ggtgcatcga
       61 ctcttccaca tattgccggg gcgcctgggc cggcggacat tcgtcgaaag ccatgatgat
      121 atcggcgccg atgcttcgct ggatatcgat gaccgattcg ggtgtaaatg tgtgcctcga
      181 gccgtcgata tgcgactgga atgtaactcc gttctcggtc agtttgcggc tatcggccag
      241 cgaatacacc tggtagccgc cactgtcggt taaaataggc ccttcccagc cgttgaaacg
      301 atgaagtccc ccggccgact cgatgaccgg catcccggga cgtgtatata aatgataggt
      361 attgcctagg attatttcag ccctgacaac gtctttcaaa tctttgaaat gtaatgcttt
      421 aacggttcct gcagtaccaa ccggcatgaa gatgggagta tgtattaacc catgcccggt
      481 ttcgaggatt cctgctcttg cattcgactg aatatcagta gcttcaattg taaatctcat
      541 tgttaataaa aaatgatcaa agataaattt tattcctgcc ggattaaata attttgtttt
      601 tgagaatgat tgagtttcct tttaaaccat cggtgcatga gatactgccc ttcgcagtgt
      661 ttatcctttt cgggataaca tggctcattc agatggtata ctactgggga atcttcagca
      721 ggcttgcatt tttcaggcgt aaagccacta ctgcggatac cccgcccgta tcggtcatta
      781 tttgtgctaa aaatgaatac cggaacctga tccagaatct tccgctgatc ctggaacagg
      841 actatcccga ttacgaagtc attgtcgtta acgacgcatc ggatgacgat acgatcgaac
      901 tgctgagttc ttttacccgg aaatatagcc atctcagaat atttaacctg gaacagaacc
      961 tcaatttttt ctccggcaag aagtttccgc tttcgctggg aataaaatcc gccaggcacg
     1021 aaatcgtgct tctcaccgac gccgattgcc agccttccgg caaggagtgg atccggaaaa
     1081 tgatggagaa ttacgggcca gggaccgaaa tagtgctggg ctacggaact ttcagaaaaa
     1141 cagcaggctt tctcaaccag atcatccgct acgacgcatt cctggttgcc gttcagtatc
     1201 tgtcgtttgc cctctccggc tcgccttata tgggtgttgg ccggaacttg tcttacaaaa
     1261 aaaacctctt ctttaaaaac ggcggcttca tctctcatta taaactcaaa tccggcgacg
     1321 acgacctgtt cattaaccgc gtggcccgcc ggagaaatac ccggattgag atacatcctg
     1381 atgctcatac catttcatcg cccaagtcag gattttacca gtggatcctg caaaaaagaa
     1441 ggcattatac aaccgcaaga ttttaccggc ctgcattcaa gtttttgctc tcgctgggat
     1501 acgtctccaa gttgttgctc tacctttcat ttattgcact gatggtggtc cgctacaact
     1561 ggatgattgt tctgtccgct ttttcgctgt tttttatcag ccactggatc atcctctctt
     1621 ttgctgctaa taaaatgaaa gagaacgatc tggcggtatt atctcccatc tacgaggtgc
     1681 tgctattgat tatcagtccg ttactttata tttccaatat cgttcacaaa caggagagat
     1741 ggaaataagc cagcggatga ccggtaaagc gatcagggat tttgacctgg ttaaagctgc
     1801 cctggaaaag ggagaccaga acgcctatgc cgaactgatg aagaattacc gtgacgcttt
     1861 gtatttcatg atgctgaaaa tgaccaataa tccgcatgat gcggaagacc ttactattga
     1921 ggcctttgga aaagctttca ggaatctgcg tcagtatacc ccggattatg ctttcagtac
     1981 atggctcttc aagatcgcct caaataattg catcgatttt atccgcaaga aaaaactgaa
     2041 tgaaaatatc tcattcaatc tgaatgactt accggattcc ggtgacggac tggccgatat
     2101 tcttcctacg acatccagga atccggaaga agatatcatc cggaaacaaa aaattgaaac
     2161 cctgcgcctt gtgatcgata aactcaaacc gaactaccgc aggctgatcg aattaaggta
     2221 ttacgaggag aaatcgtacg aggagatatc ggaagcactt gatctcccta ttggtacggt
     2281 taaagctcag ctcttcaggg ccagggaatt tctttatcac tttctgagta accgtcacga
     2341 caaactatac gaggagtgat cccggaaatc aatatttttt attcggataa tatatttgta
     2401 ttaatttagt tatttcgaat gagttaatta aaacttcagc acatgaaaag actaaagatt
     2461 ttgatcatta ttctgatggg agcattgatg atcacttcct gtggtaagaa aaaagaacag
     2521 cagatcctgg atctccaggc aaaaaatgcc gaattactgg cagaatccaa ccggaaagat
     2581 tcactgatga acgaattatt tacttcgatt aatgacattg aaaggaacct ggcagatgtc
     2641 acggcccgtg aaaagctgat aaccgatgca cccgtatcgg agcagcggat gaacgaagat
     2701 gtacgcacaa aaattatgga tgagattatc ctcatcaata acataatgga agaaaataaa
     2761 aagcagattg ccgctttgaa agaccagctg aagaaatcag gcgccaaagt ggctgctctt
     2821 caggaaacca tcaatctttt aacccagcgc ctcgaagaaa aagaaatgga gattgctgac
     2881 ctgaaaaatc agcttgtacg tcaaaatttc agaattgaag aattgaatgc taccgttgat
     2941 acccttcagg aggaaaaagc ctcgcagtcg aggatcatca gtcagcagga cgccctcatc
     3001 ggggagatga acacgatctg gtacgtttca ggtccgaaaa aagaacttct ggaaaaaggg
     3061 ataattgaga aagccggggg aatgttgagc ggaaaaatga agatgagcga cctgatcaac
     3121 ccgggatatt tcgttaccgc cgatatgcgg aagatttcgg taataccttt tgaaggtgac
     3181 aaagcggaaa tggtaacggt tcatcccgaa ggttcataca aatttttgat ggaaggcgat
     3241 acatttaaag ggattgagat tctgaatccg ggggaattct ggaagtcatc ccgctttctg
     3301 gtgattatga ctaaatgatg atgaaattga ttgattttca aaaagctata gcaactggta
     3361 tcggagatgt cctgcccgaa ccgaagcctt atgatagaag tgtaagccac gcacccagaa
     3421 gaaaagacat cctgacgccg gaagagaaaa ggctggctct gcgcaatgct cttcgctatt
     3481 ttaaaccgga acatcatccg gtgctggcat ccgaatttgc gacggaactt gagagattcg
     3541 gccgcattta catgtaccgg ttcaggccgg attacccgat gtttgcacga cccatcggcg
     3601 aatatcccca tcgctctttg caggccgccg ccattatgct gatgatccag aataacctcg
     3661 atccggctgt tgcgcagcat ccgcatgaac tgatcaccta tgggggtaac ggggctgtat
     3721 ttcaaaactg ggcgcagtac ctgctgacaa tgagatattt gtcggaaatg accaatgaac
     3781 agaccttggt aatgtattcg ggtcatccgc tcggcttatt tccgtctcac cccgatgcac
     3841 ccagggtggt ggttaccaat ggaatggtca ttccgaatta ttcttcgcag gacgattacg
     3901 aaagatttaa tgcgctcggc gtttcgcaat atgggcagat gacagccggt tcgtacatgt
     3961 acatcggccc gcaggggatt gtgcacggta caacgatcac catcctcaat gcaggacgca
     4021 ggcttgatcc gggaaacgga agcctggcag ggaaggtttt tgttacttcc ggcctgggcg
     4081 gtatgtcggg tgcgcaggct aaagcagcgg taattgccgg cgcagtggga gtcgttgcag
     4141 aagttaaccc cgcggcagtc agtaaaaggc atgaacaggg atgggtagac gaagtgttca
     4201 ctgatcttga cctgttactc gccaggattc aaaaagccag aaccctccgg gaagccgttt
     4261 ccattgctta ccagggaaat attgtcgacc tgtgggaaaa gatggcggaa cagcaatttg
     4321 acgtggaact gggctccgat cagacctcgc tgcataaccc ctgggcaggc gggtattacc
     4381 cggccgggat cacttttgaa gaatctaacc gcctgatggc ggaagatccg gcccgtttca
     4441 gggaattggt gaaaggatcg ctcatccgcc atgtgaaagc t
//
LOCUS       DAOPNH010000273         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23059_length_4438_cov_5.973306, whole genome shotgun sequence.
ACCESSION   DAOPNH010000273 DAOPNH010000000
VERSION     DAOPNH010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23059_length_4438_cov_5.973306"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..559
                     /locus_tag="PKZ74_12345"
     CDS             11..559
                     /locus_tag="PKZ74_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM93589.1"
                     /translation="MNEEKSLLVPEEVIMSKIYLIRDQKVMLDADLAELYEVETRRLN
                     EQVKRNIERFPDDFMFQLTKEEFEHLKSQIATSSWGGRRKLPFAFTEHGVLMLSSVLN
                     SNRAIRVNIQIMRIYTRIRQMMLTHKDILLRQELIEKTLSTHDDKILLIFGYLKQLEQ
                     DRQQKQEQQERKRIGFRRDGRE"
     gene            complement(635..1468)
                     /gene="tsf"
                     /locus_tag="PKZ74_12350"
     CDS             complement(635..1468)
                     /gene="tsf"
                     /locus_tag="PKZ74_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPM93590.1"
                     /translation="MANITAAEVNKLRQITGAGMMDCKAALIESDGDTEKAIEVLRKK
                     GQKVAAKRADKEATEGIVIAKTNDANDFGALVKVSCETDFVAKSDDFIHYVKGVIDLA
                     IAKKPASTADLLALDLNGRTVEQNLTDLVGKIGEKMELAGYHTVGAPLVSAYNHLGNK
                     LATLVGMNQKSPAVAEAAHNVAMQIAAMSPIAIDRDDVDKSVIEKEIEIGMDQARNEG
                     KPENMLEKIAIGKLNKFYNENTLLNQEYVKDNTKNIRTYLQGIDKDLTVTAFVRQKLG
                     D"
     gene            complement(1488..2384)
                     /gene="rpsB"
                     /locus_tag="PKZ74_12355"
     CDS             complement(1488..2384)
                     /gene="rpsB"
                     /locus_tag="PKZ74_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPM93591.1"
                     /translation="MARTTFEELLDAGVHFGHLRRKWNPNMAPYIFMERNGIHIIDLY
                     KTMAKLEEAASALKQISKSGRKILFVATKKQAKEIVATQVQKVGMPYVVERWPGGMLT
                     NFATIRKAVRKMGSIDKLMSTSNFKSLSKRERLQINRERQKLEKQLGSIAELNRLPSA
                     IFVVDILKEKIAIAEARKLNIPTFAIVDTNSDPNIIDFPIPGNDDASKSIELILRVLV
                     QAVEEGLAERKIDKEARGEDDDRDEFDQPNLLVDGLDDEEDAEAMRKKGLEKLAKAKK
                     EEEDRMAEAGKRPRRGLTKTRK"
     gene            complement(2502..2888)
                     /gene="rpsI"
                     /locus_tag="PKZ74_12360"
     CDS             complement(2502..2888)
                     /gene="rpsI"
                     /locus_tag="PKZ74_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPM93592.1"
                     /translation="MEVINATGRRKTAIARVFLKEGNGQITVNKRDVKEYFPTAVLQY
                     TVHQAFEITQTENKFDLNVNLDGGGYKGQAEALRMAIARALVKLNPEFRKALKDKGLL
                     RRDPRMVERKKPGQPGARRRFQFSKR"
     gene            complement(2894..3349)
                     /gene="rplM"
                     /locus_tag="PKZ74_12365"
     CDS             complement(2894..3349)
                     /gene="rplM"
                     /locus_tag="PKZ74_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPM93593.1"
                     /translation="MDTLSYRTVSANKETVEKQWILINAEGQVLGRLASQTAKILRGK
                     NKPYFTPHVDCGDNVVIINAEKVVLTGNKLEGKDYVHYSGYPGGDKHHSPKEMLKKRP
                     TYLIEEAVRGMLPRTRLGRAIFGNLHVYAGPEHPHQGQAPKEIDINTIK"
     gene            complement(3447..4331)
                     /locus_tag="PKZ74_12370"
     CDS             complement(3447..4331)
                     /locus_tag="PKZ74_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93594.1"
                     /translation="MISPDIGSIDKLNFAPSGDVYAVGWGGILRSTNGGSVWDTLYDP
                     VNSQFFSDIDFGLDGEIYAVGGNFGEGNGFYRSLDNGLTWEAIGFPDDFRRSLRVNSE
                     GNILVGGFGYNGMYQSTDQGSTWTFCSSIIADVMESFNDDQLIAGRCVNGYRGCWYSS
                     DWGQSWVTLTDDQFNPRVDQISVSPSGYAYIKADTSGSLDYKLFRSVNPLVGINSVEK
                     NAGFVLYPNPMKDQLFISGNQIKKIDKIAIYSLTGQKMAVSKYQTNPILFPEIIPGIY
                     IIEIQSGSGVFRKKIIHY"
BASE COUNT         1084 a         1075 c         1006 g         1273 t
ORIGIN      
        1 atcaatggtt atgaatgagg aaaaatcgtt actggttccc gaggaggtga tcatgagcaa
       61 aatttatctg attagggatc aaaaggtgat gctggacgct gatttagcgg aattatatga
      121 agttgagacc cgtcgtttaa atgaacaggt aaaaagaaat attgaacgat tccctgatga
      181 ttttatgttc cagcttacca aagaggagtt cgaacacttg aagtcgcaaa ttgcgacttc
      241 aagttggggc ggcagacgaa agctcccgtt tgcgtttact gagcacggcg tgctaatgct
      301 gtcaagtgtt ttaaacagca atcgtgcgat cagagtgaat atacaaatca tgcgtattta
      361 cacaagaata cgccaaatga tgctaactca taaagatatt ttgttgagac aggaactaat
      421 tgaaaagacc ctctcaaccc acgacgacaa aatcctttta atcttcgggt acctgaaaca
      481 actcgaacag gaccggcagc agaaacagga gcaacaggaa agaaaacgca tcggcttcag
      541 gcgggatggc cgggagtagc gccaaagaaa taaaaaaagg ctgcccgatg agacagcctc
      601 tttatatttt caggttgaat aaccggttgc gctactagtc gcccagcttc tgcctaacaa
      661 acgccgttac ggtcaggtct ttatcgatac cctgaagata tgtccggata tttttcgtgt
      721 tatccttcac atattcctgg ttaaggaggg tattctcgtt atagaactta ttgagcttgc
      781 cgatggcgat cttttccagc atattctccg gctttccttc attgcgtgcc tggtccatac
      841 cgatctcgat ctctttttcg atgacgctct tgtcgacatc gtcgcggtcg atggcaatcg
      901 ggctcatggc agcgatctgc atagccacat tgtgtgccgc ttcggctacg gcagggctct
      961 tctggttcat cccaacgagc gttgcgagct tgttgccaag atgattgtag gccgatacta
     1021 acggagcgcc gacagtatga tatcctgcca gttccatttt ttcgccaatt ttaccaacca
     1081 gatccgtcag gttctgctca acagtccggc cgttcaggtc aagggccagc aggtcggcgg
     1141 ttgaggccgg ctttttggct atggccaggt cgataacgcc tttcacgtaa tggatgaaat
     1201 catcgctctt agccacgaaa tcggtctcgc agctaacttt caccagcgcg ccaaagtcat
     1261 tggcatcatt ggtctttgca atgacgatcc cctcagttgc ttctttatcg gcacgtttag
     1321 cagcgacttt ctgacctttc ttgcgcagca cttcaatcgc tttctccgta tcgccgtcgc
     1381 tttcgatcag ggcggctttg caatccatca tgccggcccc ggtgatctgt ctcagtttat
     1441 taacttcagc agcagtaata tttgccataa tcaaatttct ttaaatgtta ttttctggtt
     1501 ttagtaagcc ctctgcgcgg acgtttacct gcctcggcca tccggtcttc ttcttctttc
     1561 ttggctttgg ccagtttctc aagtcctttt ttacgcatag cttccgcatc ttcttcgtcg
     1621 tccagtccgt cgaccaacag gttcggctga tcgaattcgt cacggtcatc atcttctccg
     1681 cgggcttcct tatcgatctt tctttctgca agcccctctt ccacagcctg taccaggacc
     1741 cggaggatca gctcgatcga tttcgacgcg tcgtcgttgc ccgggatggg gaaatcgatg
     1801 atgttcggat cggaattggt atcaacgatc gcgaaggtgg ggatattcag tttccgggct
     1861 tcggcgatgg cgattttttc tttcaggata tcgaccacaa aaatcgccga gggaaggcgg
     1921 ttgagttcgg cgatactgcc gagctgtttc tccagtttct gcctttcgcg gttgatctgc
     1981 agcctttccc ttttcgacag gctcttgaag ttgctggtcg acatcagttt gtcgatcgag
     2041 cccattttgc gcaccgcctt gcggatagtg gcaaagttgg tcagcatacc gcccggccag
     2101 cgttcgacca catagggcat tcccactttt tgcacctggg tggcgacgat ttccttggct
     2161 tgtttcttgg tagcgacaaa taatattttg cgccccgatt tggagatctg tttcagggct
     2221 gaagccgctt cttccagctt ggccatcgtt ttgtagaggt cgatgatgtg gatcccgttg
     2281 cgttccataa aaatataggg cgccatgttg gggttccatt tcctgcgcag atgcccgaaa
     2341 tgtactccgg catccagtaa ttcttcgaaa gttgttcttg ccattaattt gtagatttat
     2401 gtttaccttc tgattaagca acccccgggt agcagtccgc atgcagcggt gatggtcccg
     2461 ggcgtttaga tactaaacac tctttatcgg ggagcgatcc gttaacgctt gctgaactgg
     2521 aagcgcctgc gagcaccggg ctggccgggt ttcttgcgtt ccaccatgcg gggatctctg
     2581 cgtaagaggc ctttatcttt taatgctttc cggaattcgg ggttcagttt gaccagtgcg
     2641 cgggcgatgg ccatacggag cgcttcggcc tggcctttgt aaccgccacc gtcgagattg
     2701 acgttcaggt caaatttatt ctctgtctgg gttatttcaa atgcctggtg taccgtatac
     2761 tgaagcactg cggtcggaaa atattctttc acgtcacgct tgttcactgt gatctggcca
     2821 ttgccttcct ttaaaaatac gcgggcgatg gctgtctttc ttcttccggt tgcgttaatg
     2881 acttccatat tgattattta atggtattga tatcgatttc tttaggagcc tggccctggt
     2941 gcggatgctc gggaccggca taaacgtgaa ggtttccgaa gattgcccgt ccgagcctcg
     3001 tcctggggag catgcccctg acagcttcct cgatgagata ggtcggcctt ttcttcagca
     3061 tctccttcgg agaatggtgc ttgtcaccgc ccgggtaacc gctgtaatgc acataatctt
     3121 ttccttccag cttatttcct gtcaggacga ccttttcagc attaataatc acaacattgt
     3181 ccccacaatc cacatgaggt gtaaaatagg gcttgttttt tccgcggaga attttagcgg
     3241 tctgtgatgc cagccgtccc agcacctggc cttcagcgtt gatcaaaatc cactgtttct
     3301 caacagtttc cttattcgcg ctgacggttc tgtaactcaa agtatccatc gatggctatg
     3361 tttcaaatat taaatcgttt accttctgaa aatggggtgc aaagatagaa tgattttttg
     3421 gtattaacaa acagaagcgg aaaaaattaa taatgaatga ttttcttcct gaaaacgcct
     3481 gatccagatt gaatttctat aatataaatc cctggaataa tctcaggaaa caaaatcgga
     3541 ttagtctgat atttactaac cgccatcttt tgcccagtca aactatagat agcaatttta
     3601 tctatttttt tgatttgatt acctgatata aaaagttgat ctttcatagg attgggataa
     3661 agtacaaagc cagcattctt ctccacacta ttaattccga caagcggatt tacggaccga
     3721 aaaagtttgt aatcaaggga accggacgta tctgctttaa tataggcata tccagatgga
     3781 gaaacagaaa tctgatcaac acgtggattg aactgatcat cggtgagtgt cacccatgac
     3841 tgtccccaat cgcttgaata ccagcatccc cgatatccat ttacacaacg gccagcaatc
     3901 agctggtcat cgttaaatga ttccatgaca tctgcgatga tcgatgagca aaaggtccat
     3961 gtggaaccct ggtcggtcga ctgatacatt ccattatatc caaatcctcc tacaaggata
     4021 tttccttctg aattaacacg taacgatcgt cgaaaatcat caggaaatcc gatggcttcc
     4081 caggtcagtc cattgtctaa tgaccgataa aatccattgc cttcgccgaa attgcctcca
     4141 accgcatata tctcaccatc caggccaaaa tcaatatcgc tgaagaactg agaattcacc
     4201 ggatcgtaaa gggtatccca aactgagcct ccattcgtgg atctgagaat ccctccccaa
     4261 ccgactgcat agacatcccc ggaaggtgca aagttcaatt tatcaatgct gcctatgtct
     4321 gggctgatca attggtaatg atcgccatta tcatcagata caaagatgcc aaaactctca
     4381 attaaaaaaa ggagaattta tggccgatgc gaataacaaa gagataaagt taaaaatt
//
LOCUS       DAOPNH010000274         4430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23113_length_4430_cov_8.979657, whole genome shotgun sequence.
ACCESSION   DAOPNH010000274 DAOPNH010000000
VERSION     DAOPNH010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23113_length_4430_cov_8.979657"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..767
                     /locus_tag="PKZ74_12375"
     CDS             303..767
                     /locus_tag="PKZ74_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HPM93595.1"
                     /translation="MKNSNQKLIIEQVDRKLQPFKSLDAMVVPPKGWINTVRTALRMS
                     LRQLGNRLNISPQSVKELENREANGSLTIKSLKEAGLALNLRLVYGFIPMDGSIEKMI
                     EARAFEVAREIVARTSQSMKLENQENKEERLKKAIEMKAREIMDNMPKYLWE"
     gene            758..1354
                     /locus_tag="PKZ74_12380"
     CDS             758..1354
                     /locus_tag="PKZ74_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HPM93596.1"
                     /translation="MGIELNYIEGQTPVDEDEKYGLRILSITTREELDEFEQLNIEKA
                     VEWTMRRKFSQEIIFTEQFVKELHLRMYDDVWKWAGKFRTTNKNIGVDWQIISVSLKQ
                     LLDDCKFWIESNTFPEEEIAVRFSHRIVCIHCFPNGNGRHSRLIADVIISHIFNKPVF
                     SWGSSNLVKQGEVRKQYIDAIQSADKGNILPLLYFARS"
     gene            1987..3138
                     /locus_tag="PKZ74_12385"
     CDS             1987..3138
                     /locus_tag="PKZ74_12385"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93597.1"
                     /translation="MIIERTIQKSIVERISESGHPRKVIIIYGARQVGKTTLVKEILS
                     GYDLKKEYFNCDYLDVQSAFSYENAGNFGNIVKNLRLIILDEAQRINNIGMVLKILHD
                     EFPQLKIIATGSSSFELSGKISEPLTGRKLVYQLYPFSFRELEAVYSKMDAQRLISKI
                     MRFGTYPSVILEDDQHAVENLNEIASSYLFKDIFTYQSLKKPEIILDLLKLLAFQVSG
                     EVSYTELARKLKVDQTVIQRYIQLLEDSFILFRLQALRKNLRTEVGKTRKVYFWDIGI
                     RNILIQNTNTLDFRDDHGKLWENFCVSERMKYLNSRPSGQIQSFFWRTYNQKEIDYIE
                     ESSGSFSAFEFKWSSKKAGKLPVDFSQAYSCSEIRNANPGNFSDVLYDI"
     gene            3245..3748
                     /locus_tag="PKZ74_12390"
     CDS             3245..3748
                     /locus_tag="PKZ74_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPM93598.1"
                     /translation="MSRGFVKEDDQEEVPMVPPRAYLPPGVINYVTPAGMEELLAEQQ
                     ALMDEIKNLEITSEKERRIAVNHITARLNLLNNRISEARIIDPEDQPKDEVRFGAAVT
                     LKTSDTNEIQTFRIVGVDEADISGGKISFISPLARVLTNKKTGEKVVLKRPKGDQVLE
                     ITGIAYL"
     gene            3781..>4430
                     /locus_tag="PKZ74_12395"
     CDS             3781..>4430
                     /locus_tag="PKZ74_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93599.1"
                     /translation="MKKGNRSFENNSFERIVTVSLLLAFVLVLVTGILSFVKLNGIIS
                     TIDDTIRPNRSINLIKDIYNDLMQAENHVKSYNLTSSPEDLDRYNGLINTTAEKVKNL
                     KGLVKTEEVFAPYADTMEVLTEKKFNGLDMLVIIKDTYPVKEAIQDFMYDFSGKILPP
                     GMADSVDSKSFEQMAEKIRKTTKEALAAEYTRNELEQEWTTYDRVITDKLRAMISGL"
BASE COUNT         1366 a          869 c         1001 g         1194 t
ORIGIN      
        1 tttttgattt gacgatttga cgatttgacg atttggcgat ttgacgattt gacgatttga
       61 cgatttttcg gtttgcggtg tgttttgagt ggactggccg atcatcctca tcactacccg
      121 ccggtttagt ctggcccatt taacccactt aggatcaatt cgcttgaggg gccactgaca
      181 accggccttc gatggtcaga tctaccggct tttcaactta tttagaatat attcttaaaa
      241 tttgtttgct actttcaggt tatatcctta attttgcctt tcatttttaa gacaataccc
      301 ttatgaaaaa ctcaaatcaa aaattaataa tagaacaggt cgacagaaaa cttcaaccgt
      361 tcaagagcct tgatgctatg gtcgttcctc caaaaggctg gataaatacg gtcagaactg
      421 cactgagaat gtcgttaagg caacttggga acaggcttaa tatttctccc cagagcgtta
      481 aagagcttga gaacagggag gccaatggat cattgactat taaatcattg aaagaggccg
      541 gactggcttt aaacctgaga ctggtgtatg gctttatccc gatggatgga agcattgaga
      601 agatgataga ggcccgggct tttgaagtag ctcgtgaaat cgtcgcccgc acttcccaat
      661 ccatgaaact tgagaaccag gagaacaaag aggaaagact gaagaaagca atcgaaatga
      721 aggcaaggga aataatggac aatatgccaa aatatttatg ggaatagaac tcaattatat
      781 tgaaggccag actccggttg atgaggatga aaagtatggt ctccggattc tttccatcac
      841 cacccgtgaa gaactcgatg aatttgagca gttgaacatc gagaaagctg ttgaatggac
      901 tatgagaaga aaattcagtc aggagattat ttttacagaa caatttgtca aagaactgca
      961 cttgcggatg tatgatgatg tttggaaatg ggcaggaaaa ttcagaacaa cgaataaaaa
     1021 cattggagtg gactggcaaa taatctcagt ttccttaaaa caattattag atgattgtaa
     1081 attctggata gagagtaata cctttcccga agaagaaatt gcggtaaggt tcagtcatag
     1141 aattgtctgt atccattgct ttccaaatgg aaatgggaga cattccagat taatcgctga
     1201 tgtgatcatc agtcatattt tcaataaacc tgttttctcc tggggaagtt caaacctggt
     1261 gaaacaagga gaggtaagaa agcagtatat agatgctatt caatcggctg ataaaggaaa
     1321 tattctccct ttgttgtatt ttgcgaggtc ttaagttacc cgatcaagcc ataatgattt
     1381 agaaattagc gaacctcggg gagccgcaaa aaagcccgtc ccgaattctc gggagggctt
     1441 ttttgatttg acgatttgac gatttgacga tttggcgatt tgatgatttg atgatttggt
     1501 gatttgatga tttggtgatt tgatgatttg gtgatttggt gaagtaattt ttttaattgg
     1561 cagatacgtc aaaaatccaa ttttttgata tcaattatag tatggccaaa tcgaaaaacc
     1621 gggacaacgc caaaccgacc aatcggaata tcggtaaatt cctggactaa tttgtgtgaa
     1681 attatcatat tattttgatt ttttcataga tttcggtact tattgcaata aatcaactca
     1741 cgttgtttga aacgaagaat tagtgaacag ctcattttgt ggaaagacaa aagggaccgg
     1801 atgccgctta ttattaatgg cgcaaggcag gtgtggattt agctgagtat tttccggttc
     1861 ggcatatgct cctatttatc cggatctttc gatcttgatg ataaataaaa cgaaaaatgt
     1921 catgcaaata gcattaattg catgacttgt gtcttgctat ttagtatctt tgcaaaaaac
     1981 aaacctatga ttattgaaag gacaatccaa aaaagcatcg ttgaaagaat ttccgaatcc
     2041 ggccatcccc ggaaagtaat catcatttat ggcgccaggc aggttggcaa aacaactctg
     2101 gttaaagaga tcctttccgg ttatgatctg aaaaaggaat actttaactg cgattacctg
     2161 gatgtccagt cggctttttc atatgagaat gcaggaaact tcggcaatat tgtaaaaaac
     2221 ctccggttaa tcatcctgga tgaagcgcaa agaatcaata atatcggaat ggtcctgaaa
     2281 atactgcatg atgagtttcc tcaattaaaa attattgcaa caggatcatc cagctttgaa
     2341 ctttcaggaa agatcagcga gccactaaca ggcagaaaac tggtttatca gttatatcct
     2401 ttctcgttca gggaattgga agctgtttat tcaaaaatgg atgcacaacg attgatcagt
     2461 aaaatcatgc gtttcggaac ctatccttca gtaatcctgg aagatgacca gcacgctgtg
     2521 gaaaacctga atgaaatcgc ttcaagttat ttattcaagg atatttttac ttatcaatcg
     2581 ctgaaaaaac ctgagatcat tcttgacctg ctgaaacttc tggccttcca ggtttcaggt
     2641 gaagtttcct atacagaact ggctcgtaaa cttaaagtcg atcaaactgt gatccagcga
     2701 tatatacagc ttttggaaga tagtttcatt cttttcaggc tccaggccct gagaaagaat
     2761 ctcagaacag aagtggggaa aacccgtaag gtttatttct gggatatcgg aataagaaac
     2821 atccttattc aaaataccaa cacccttgat ttcagagatg accatggcaa attatgggag
     2881 aatttctgtg tttctgaaag gatgaaatat ttaaacagcc gcccgtccgg gcaaatccag
     2941 tcattcttct ggcgaacata caaccagaaa gaaattgatt atatcgaaga gagtagcggc
     3001 agtttcagtg cgtttgaatt caaatggtca tcaaaaaaag ccggcaaact gccagtggat
     3061 ttttcacaag cttactcatg ctccgaaatt agaaatgcga atccgggaaa cttctcagac
     3121 gtcctctatg atatttagat atcagccaac cgcgggggct gttggatcgg cgaaagcacg
     3181 atcaaccgat cgtcgatcga atgatccggc attttcgtac ttttacccct ggtaatttcg
     3241 acccatgagc agaggatttg tcaaggaaga cgaccaggaa gaggtcccga tggtgccgcc
     3301 cagggcctat ttgccgccgg gagtcattaa ctatgttacc ccggcaggta tggaagagct
     3361 gctggccgag caacaggccc tgatggatga aataaaaaac cttgagatca ccagtgaaaa
     3421 ggagagaaga attgcggtga accatatcac ggccagactg aatctgctca acaaccggat
     3481 cagtgaagcc aggatcatcg atccggagga tcagccgaaa gatgaagtgc ggttcggcgc
     3541 tgcggtgacc ctcaaaacat cagataccaa tgaaatacag actttccgga tcgtaggtgt
     3601 cgacgaggcc gatatctccg ggggaaagat ctcttttata tcgcccctgg cacgggtgct
     3661 gaccaataaa aaaaccgggg agaaagttgt tctgaaacgt cccaaaggtg accaggtact
     3721 tgaaatcaca ggtattgctt acctttaatc agcgtcagga tatcgttgaa cccatcaatc
     3781 atgaaaaaag gaaacagatc atttgaaaat aacagtttcg aaaggatcgt aacggtttca
     3841 ctgctgctgg cttttgtgct ggtgctggtc accggtatcc tctcctttgt taagctgaac
     3901 ggcatcatct ctacgattga tgatacgatc cgcccaaacc ggtcgatcaa cctgatcaaa
     3961 gacatttata acgacctgat gcaggctgag aaccatgtca aatcctacaa tctcaccagt
     4021 tccccggagg atctcgaccg ctacaacgga ttgatcaata ctaccgcgga aaaggtgaag
     4081 aacctgaaag gcctggtcaa aaccgaagag gtttttgccc cgtatgccga tacgatggag
     4141 gtgctgaccg aaaagaaatt caacgggctg gatatgctgg tgatcatcaa agacacttat
     4201 cctgtaaaag aagccatcca ggatttcatg tatgattttt ccggtaagat tttgcctccc
     4261 ggtatggcag attccgttga ttccaaatcc tttgagcaaa tggcggaaaa gatcaggaag
     4321 acgacaaagg aggcgctggc cgcagaatat acgcgcaatg agctggaaca ggaatggaca
     4381 acctacgacc gggtgatcac cgataagctg agagctatga tctccggcct
//
LOCUS       DAOPNH010000275         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23152_length_4425_cov_6.044165, whole genome shotgun sequence.
ACCESSION   DAOPNH010000275 DAOPNH010000000
VERSION     DAOPNH010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23152_length_4425_cov_6.044165"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKZ74_12400"
     CDS             complement(<1..222)
                     /locus_tag="PKZ74_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93600.1"
                     /translation="MGLDTLDPLPMNRVDFMLNLAGLLLWLEWRAVRLGAPLAAAGSI
                     SAVLKHAKPRAARRWLHLAGLVALLGIRCV"
     gene            293..1765
                     /locus_tag="PKZ74_12405"
     CDS             293..1765
                     /locus_tag="PKZ74_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPM93601.1"
                     /translation="MKHRHLVLWATAIALLLSSTGCISLNGADATGTPIPFGPVPTER
                     QLRWHAMEFYGFLHFTVNTFTDKEWGYGDESPDLFHPTAFDADQLVESAADGGLRGLI
                     LTCKHHDGFCIWPSRYTEHSVKNSSFMDGKGDVVKAVSDACRKYGLKFGIYLSPWDRN
                     QPAYGSAAYIDYYRNQLGELLTDCGPVFEIWFDGANGGEGFYGGARENRQIDRRTYYQ
                     WPLTIDMARQVQPDVLFFSDAGPDIRWCGNESGFAGETNWCLINPDTLYAGKSGIEGL
                     LNSGSADGTRWIPAEVDVSTRPGWFYHPSQDSQVKSPEKLFEIYLSSVGRGSNLLLNI
                     PPDRRGLLHENDVKSMLGFKKLRDEAFAYNLAFNARVTASSFRGSSPQFNPANLVDNN
                     KETFWATDNTITTSDIVIDLGIEKCIRYLVLSEYIRLGQRVRSFEIEVLKDKDWVTVA
                     TGTTIGYKRIISLDPVFAQKIRISITGSKACPVLSEIEVY"
     gene            complement(1813..2088)
                     /locus_tag="PKZ74_12410"
     CDS             complement(1813..2088)
                     /locus_tag="PKZ74_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93602.1"
                     /translation="MKFIYYDELVSGKEEIIFPDSLSREDQKKLITERLKKGPDDTVQ
                     SISGMPYTRDDLIHAIENDEDMGNMTMEAELIMLRDLLRLIAGQRAK"
     gene            complement(2229..3356)
                     /locus_tag="PKZ74_12415"
     CDS             complement(2229..3356)
                     /locus_tag="PKZ74_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93603.1"
                     /translation="MKIGKLNFKDYAAKKPVLLSPSGKFLTIKEVTATPKFEFGSLHT
                     LSGESQAKLALERYKLEPDFKLGIFEQGVMTKADVMKHIQDGTEFGQLAVQVEMQYCN
                     ELMQSLKMTTVPAFPKLPDKPLPAVPEWKQIKRCFWFRIKNIVLFCENTTDAVTTPFA
                     LYRIANVHSVFAARGFNIVALTGTNVTRTNFEATAKKPLTVYLSGIGHGNYDVYTGHA
                     GGIILKVGAYDPAEVKGKAIHFLSCRTAARLGPDTVAKGAKCYAGYDENFTFVWDNPA
                     TPINEVDLFKKSDSIFDIYMANGFTAQQAYNAAIATFNACIALVPGTAAASWLSYDRD
                     HFRLLGDPNTRISPYRFVRICLPLKTPLHEEMLAEVGELMD"
     gene            3986..>4425
                     /gene="nuoE"
                     /locus_tag="PKZ74_12420"
     CDS             3986..>4425
                     /gene="nuoE"
                     /locus_tag="PKZ74_12420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPM93604.1"
                     /translation="MRIERISPAVAQEKTEDIDLSRLDHLIEKYKNRKGNLIPLLQGT
                     QAIFGYVPRDAFVKISRETGLELSDMYGVATFYAQFRLKPVGKHIIKVCHGTACHVQN
                     ANTISDSLEEALKIKDGETTEDGMFTLESVACLGCCSLAPVMMIG"
BASE COUNT         1044 a         1158 c         1122 g         1101 t
ORIGIN      
        1 cacacaccga atccccagca gcgccaccag gcccgccagg tggagccacc gccgcgccgc
       61 gcgcggcttg gcgtgcttca ggaccgcgga aatcgagccc gccgccgcca gcggcgcgcc
      121 caaccgcacc gcccgccact cgagccacag aagcagcccc gccagattca gcataaaatc
      181 aacgcgattc atcggcagcg gatcgagtgt gtccagaccc aacccagggt tcaatcatca
      241 attcgccggc ctgtctggac tcctggatgc gccgccgcta gactgcgcgc ccatgaaaca
      301 ccggcacctc gttctctggg ccactgcgat cgctctgctc ctctcttcga ccggctgcat
      361 ctccctcaac ggcgccgatg caaccggcac ccccatcccg ttcggacccg tcccgacgga
      421 gcgtcaactg cgttggcacg cgatggagtt ttacgggttt cttcacttca cggtgaacac
      481 gttcaccgac aaggaatggg gctacggcga cgaatccccg gatctcttcc acccgaccgc
      541 cttcgacgcc gaccaactgg tcgagagcgc ggccgacggc gggctccgcg gcctgatcct
      601 gacctgcaag caccacgacg gcttctgcat ctggccttcc agatacaccg aacattcggt
      661 taaaaacagt tcatttatgg acggcaaagg cgacgtggta aaagcagttt cagatgcttg
      721 ccggaaatac gggctgaaat tcgggatcta tctctcgccc tgggaccgga accagccggc
      781 atacggttct gcggcataca tcgattacta ccggaaccag cttggcgaac tgttaacgga
      841 ttgtggccct gtgttcgaga tatggttcga cggagccaac gggggagaag gcttctacgg
      901 cggcgcaaga gagaaccggc aaatcgaccg caggacttac taccaatggc ctctgacgat
      961 cgatatggca aggcaggtcc agccggatgt gctgtttttc agcgatgccg gccccgatat
     1021 ccgctggtgc ggaaatgagt cgggtttcgc cggcgaaacc aactggtgcc tgatcaatcc
     1081 cgacacactg tatgcaggga aaagtggcat tgaagggttg ttgaacagcg gcagcgcaga
     1141 cggcacccgg tggatacccg cagaggtcga tgtctccacc cggcccggct ggttttatca
     1201 tccatcccag gattcgcagg tgaaatcccc ggaaaagctg tttgagatct atctcagctc
     1261 ggtgggccgg ggctcaaacc tgctgctgaa tatccctccc gatcgtcgtg gattgcttca
     1321 cgagaacgac gtgaaatcca tgctgggttt taaaaagcta cgggatgaag cctttgctta
     1381 caaccttgcc tttaatgccc gggtcactgc cagctcattc cggggtagtt ccccccaatt
     1441 caaccctgcc aacctggtcg ataacaataa ggaaaccttt tgggccaccg acaatactat
     1501 cacaacttct gacattgtca ttgatcttgg tattgagaaa tgcatccgtt atcttgtcct
     1561 tagtgaatat atccggctcg ggcagcgggt gagatcattt gagattgaag tgttgaagga
     1621 taaggattgg gtcactgtgg ccaccggaac aacaataggc tataagcgga tcatcagcct
     1681 tgatcctgtt tttgcacaga agatccggat cagcattacc ggttccaaag cctgccccgt
     1741 actgtctgaa atcgaggttt attgaaggtc gttagctcgc tgcgcccgcg tcatttggtg
     1801 gtgatttagg aatcatttcg ctctctgtcc cgcaattaga cgcaggaggt cgcggagcat
     1861 gatcaattcg gcttccatag tcatattccc catatcttca tcgttttcaa tcgcatggat
     1921 caggtcatca cgggtgtaag gcatcccgct gatcgactgg actgtatcat ccgggccttt
     1981 tttcagcctt tcggtgatca gttttttctg atcttcccgc gaaagggaat caggaaagat
     2041 tatctcctcc tttcctgaaa ccaattcatc ataataaatg aatttcatcc ggtcaggatc
     2101 atagcataag ggttgctttg ccaaaaaatt gttcataact cgatagtatc caatattttt
     2161 ttctggtgaa agggatcacc gcctggcggc ggtgatcccg tgatcttgtc ataaaagtaa
     2221 tcaaattttc aatccatcag ctctccgact tccgccagca tttcttcatg caacggggtt
     2281 ttcagcggca gacagattct cacaaagcgg tacggcgata tccgggtgtt cgggtcgccc
     2341 agcaaccgga aatgatcgcg atcgtacgac agccagctgg ccgctgccgt cccgggaacc
     2401 agggcgatac aggcattgaa agtggcaatg gctgcgttgt aggcttgttg cgctgtgaaa
     2461 ccgttagcca tgtaaatgtc gaaaatgctg tcggatttct tgaagagatc gacctcattg
     2521 atcggcgtgg cggggttatc ccagacaaag gtgaagtttt cgtcgtaccc ggcatagcac
     2581 tttgcgcctt tggcaaccgt gtcgggcccc agtctggcag cagtgcggca gctcaggaaa
     2641 tggatggcct tcccttttac ttctgcggga tcataagctc ccaccttcag gatgattcca
     2701 ccggcatgac cggtatagac atcgtaattg ccatgcccga taccgctcag atagaccgtc
     2761 aatggctttt tagccgtagc ttcgaaattg gtccgggtga cgttggtgcc ggtcagggcg
     2821 acaatattga atcccctggc agcaaatacg ctgtggacat tcgcaatccg gtatagcgca
     2881 aaaggagtgg tcaccgcgtc ggtcgtgttt tcgcagaaca aaacaatgtt tttgatccgg
     2941 aaccagaaac acctttttat ctgcttccat tcgggtacgg ccggaagcgg tttgtcgggg
     3001 agtttgggaa aagcgggaac tgtggtcatt ttcaatgatt gcatcagttc gttgcagtac
     3061 tgcatttcga cctgcacggc cagctggcca aactcggtgc cgtcctggat atgcttcatt
     3121 acatccgctt tcgtcatcac cccctgttcg aaaataccca gtttaaaatc aggttccagc
     3181 ttgtaccttt ccagtgccag ttttgcctgc gattccccgg atagggtgtg aagcgatccg
     3241 aattcaaatt ttggtgttgc cgtcacttct ttgatggtta aaaacttgcc ggaaggagaa
     3301 agcaggaccg gtttttttgc tgcatagtcc ttgaaattta attttccgat tttcataatt
     3361 aattgatgtt aagattttcc tactctgtta gggcttttcg gttacgccct cgttttttca
     3421 tgaaactgag ccggcaaagt ttatcatgcc agggggtcag cctgttgagt aaccaatcct
     3481 taaggtttac agtgaataaa tatcaatgtc aacaacatgt ataatatgtt tgtaattatt
     3541 ttcagaatca atagaatcaa agaaaatttc ggatgccggt ttagtgattg gcagtcgggg
     3601 ggaagtttca ggtcaacgat gccagaaggc tttttaaaga aataagaaag taaaacaggg
     3661 gggattttac tctttcggga gtttcaactt tgccacccca aacttacgaa atattaagca
     3721 aaatgtcaag tgttcagatg gatattttta aagatgtcat cagctcgctg tgcgagcgtc
     3781 attaagtaga tattaggttg tgattttgaa ttttattttt tgaatttgtt tggaatttgg
     3841 aatttggagt ttggaatttg tttggatttt ggtgcttgga atttggaatt tgtctcctct
     3901 ttagactgcc tccaaattag cccggaccac ttatggtcag aagaagaatc catactttag
     3961 cctccgcttt ttaaaggcta aaattatgcg catcgaaaga atctctccgg cggtcgcgca
     4021 ggaaaaaacc gaagacatcg acctgtcccg cctggatcac ctcattgaaa aatacaagaa
     4081 ccgtaaaggc aatctcatcc cgctgctcca gggtacgcag gctatcttcg gatacgtgcc
     4141 ccgggatgca ttcgtgaaga tcagccgcga aacaggcctt gaactatcgg atatgtacgg
     4201 cgtggctacc ttctacgccc agttccgcct caaaccggtc gggaaacata tcataaaggt
     4261 atgccacggt acggcatgcc acgtgcaaaa cgccaacacg atctccgatt cgctcgagga
     4321 agcactcaag atcaaagacg gcgaaacgac cgaagacggg atgttcaccc tcgaatcggt
     4381 cgcctgcctg ggctgctgct ccctcgcccc cgtcatgatg atcgg
//
LOCUS       DAOPNH010000276         4416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23208_length_4416_cov_5.686769, whole genome shotgun sequence.
ACCESSION   DAOPNH010000276 DAOPNH010000000
VERSION     DAOPNH010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23208_length_4416_cov_5.686769"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKZ74_12425"
     CDS             <1..385
                     /locus_tag="PKZ74_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM93605.1"
                     /translation="ADGLYVGDAPQTTVAVDAEIRIMRTVTLNAGWYYFDRYYADFSP
                     NTRTDPDDRSQPFRIPSYHLLDLQALCPFKIGNLKAEASLGCLNVLNNKYIVRGQDGP
                     SHTINDFTGFWGFGRNFFAALKVNF"
     gene            578..3919
                     /locus_tag="PKZ74_12430"
     CDS             578..3919
                     /locus_tag="PKZ74_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM93606.1"
                     /translation="MKQIYKLLMTAVPVLPAAGLNGKISTSKRLLLMIVIMISSIGVM
                     YSQVTTSGINGKIYGSNYITLPGATVILVHTPTGTQYSTITDDKGIFRIPNMQSGGPY
                     EMKISYVGYKTLQKSDITLALGQTLTFNESLTEAAVGLTEVEVIEKRDALIDGNRTGS
                     STNVNSNQITTLPSINRSITDYTKLSPQANGNSFAGRDGRMNNITIDGANFNNNFGLS
                     SKALPGGDAQPISLDAIEQISINIAPFDIRQSNFTGANVNAVTKSGTNKWSGTAYTYI
                     RGKNFYGKKIEDKEIELTDQNNLNYGLSVGGPIIKNKLFVFANFERETSDFPGILWNP
                     SDPDNGVNADPDNYISRTTVADLSTMKEYLMTTYGYDAGTYQDFGNFQTKNYKILGKI
                     DWNINKNHRFSIRYNYVKSTNDQQVNATSSPGTRSAFGRIGEMSMSFNNANYGFLNTV
                     GSLSAELNSTWSKSANKFLFTYTQIRDTRSSESDIFPFVDIYKDGNPYMSFGYELFSL
                     DNDVKNNVLTFTDNFNYYLGQHTLTVGASFDKLFFGNSYKRYGTSYYRFASPEDFYNG
                     AAPTTFGITYPYAQAGDGYAELNFGYASVYGQDEWQVLDNLKITGGIRLELPIYFNEL
                     LPNEAITELTFKDLDGNDLILDAGLWPDPKMTVSPRLSFNWDAKNDKSLQVRGGTGIF
                     TGRLPFVWFTNQPTNSGTLQNTVEITNADDLAGLTFNADPFYHLTNTDLFPSAPTKAP
                     GSIAVVDKNFKMPQVWRTDLALDVKLPWNLIGTFEAIYSKAINDVIQYNANQAIADSM
                     FFGADDRERFMNRKINSTVSNAMVLTNTKEGYSYSLTAQLAREFENGFYGFLAYTYSA
                     TKDLTSNPGSAAASAWASNPTYTNQNAPTLSYSQFSVPHRVVGCVSYGIEYAKHLKTT
                     VSLFYQGSSQGRLDYIYSNDMNGDGNSADLMYIPKDESEIIFADITNGDGEVIATAAE
                     QGAAFWQYVDQDKYLSDHKGEYAERYGVVMPWVGRFDVKILQDVFTKIGKTKHTLQFS
                     LDFLNIGNLINSDWGIYRKQTLGNYDITLLKYKSSNSEGVPTYQLNYTKTDSDGKPVF
                     PTSTYSPVLGTTSTWGMQIGIRYIF"
     gene            4177..>4416
                     /locus_tag="PKZ74_12435"
     CDS             4177..>4416
                     /locus_tag="PKZ74_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPM93607.1"
                     /translation="MTYKNYKLFLVIAFLSLRTFISAQEKPYVVMLSMDGFRWDYPER
                     CGTPNLDKIAAEGVRAESLQPAFPSKTFANHYTMAT"
BASE COUNT         1278 a         1007 c          977 g         1154 t
ORIGIN      
        1 tgccgacggg ttgtacgtgg gcgatgcacc gcagacgacg gtggccgtcg acgcggaaat
       61 ccggatcatg cgcacggtga cattgaatgc tggctggtat tactttgacc gttattacgc
      121 cgatttcagc cccaacaccc gaacggatcc ggatgaccgc agccagcctt tccggattcc
      181 ctcctaccat ctgctcgacc tgcaggcttt atgcccgttc aaaattggta atctcaaagc
      241 cgaagccagc ctgggctgcc tgaatgtatt gaataacaaa tacattgtac gcggccagga
      301 tggcccttca cacaccatca atgattttac cggattctgg ggtttcggaa gaaatttttt
      361 cgctgcatta aaagtcaatt tctgatctaa tccattgatt ttcagattat acaagttgtt
      421 aacaatgatt tcttcaacaa atttctgatt ttcaattgga tatcaggtta tcttcatttt
      481 atcaacctta tttgttaaca taattttaac aagtcatccg gcctgattct ataaattcgt
      541 ccgcaaataa accatttaaa attaaataaa agttgatatg aaacagatct acaaattact
      601 aatgacggcg gttccggttt tacctgctgc cgggctgaac ggaaagattt ccacgtcaaa
      661 gagattgctt ctcatgatcg ttattatgat cagcagcatc ggcgtgatgt attcacaggt
      721 caccacatcc ggaattaacg gaaaaattta tggctcaaat tacattactt tgcccggagc
      781 cactgtaatt ctggtacata caccgaccgg aacccagtac agtaccatta ccgacgataa
      841 agggatcttc agaattccta acatgcagag cggaggtcca tatgaaatga agatatcata
      901 tgtcggttat aagactttac agaaaagcga tatcactctc gcgctgggcc agactctgac
      961 ttttaatgaa agcttgacgg aagctgcagt aggattgaca gaagttgaag tgattgaaaa
     1021 gagagacgca ctgatcgacg gtaaccgtac cggttcttcg accaatgtca attcaaatca
     1081 aattaccacg ctgccttcca ttaacaggag catcaccgat tataccaaac tgagcccaca
     1141 ggccaatggc aacagttttg ccggtcgcga cggcaggatg aacaacatca ccatcgacgg
     1201 agccaacttt aacaacaact tcggattgag cagcaaagca ctgccgggcg gtgatgccca
     1261 gccgatcagt ctggatgcta ttgagcagat cagcataaat attgccccgt ttgatatcag
     1321 gcaatccaat tttaccggcg ccaatgttaa tgccgtcacc aaaagcggta caaacaagtg
     1381 gagcgggact gcctatactt atatccgcgg caagaatttc tacggcaaaa agattgaaga
     1441 caaggaaatt gagcttacag accaaaataa cctgaattat ggattatctg tcggcggacc
     1501 tattatcaag aataaattat tcgtctttgc aaatttcgaa agagagacct cggatttccc
     1561 cggcatctta tggaatccca gcgatccgga taatggagtt aatgcagatc cggataatta
     1621 tatctccagg actaccgttg ctgacctgag cacgatgaag gaatatttga tgactactta
     1681 tggctatgat gccggtacct accaggattt tggaaatttt caaaccaaaa actacaagat
     1741 cttaggaaag attgactgga acattaataa gaatcaccgg ttcagcataa ggtataatta
     1801 cgttaaatca accaatgatc agcaagttaa cgcaacgagc tcacccggta caagatccgc
     1861 attcggaagg atcggcgaaa tgtcgatgtc atttaataat gcaaattatg gtttcctgaa
     1921 tactgtcggt tcgctttcgg ctgaattgaa cagcacctgg agtaaatcgg ccaataaatt
     1981 cttgttcacc tatactcaga tcagggatac ccggagcagc gaaagcgaca ttttcccgtt
     2041 cgtcgatatt tacaaagacg gaaatcccta tatgtcgttt ggttatgaac tcttctcact
     2101 ggataatgat gttaagaaca atgtgctgac cttcaccgat aacttcaact attacctggg
     2161 acaacatacc ctgacggtag gtgcatcgtt cgacaaatta ttcttcggaa actcttacaa
     2221 aagatacgga accagctatt accggtttgc atcgcccgag gatttctaca atggagccgc
     2281 tcctaccaca tttggtatta cctaccccta tgcacaggcc ggcgacggat atgctgaact
     2341 gaattttggt tatgcaagcg tttacggaca ggatgaatgg caggtgctgg ataacctgaa
     2401 aattaccggt ggcattaggc tggaattacc gatctatttc aatgaactgc ttccgaatga
     2461 ggcgatcaca gagttgacct tcaaagacct cgacggaaat gacctgatcc tggatgccgg
     2521 cctgtggccc gatccgaaga tgaccgtttc accacgttta agctttaact gggatgctaa
     2581 aaatgacaag tcgctccagg tcagaggcgg tacaggtatt ttcacaggcc gcttaccctt
     2641 tgtatggttc accaatcagc cgaccaacag cggaaccctg cagaatacgg ttgaaatcac
     2701 caatgccgat gatctggccg gtttgacctt caatgccgac ccgttctatc atctgaccaa
     2761 taccgatctg ttcccgtcgg ctccgaccaa ggcacccggc tcgattgccg ttgtggataa
     2821 gaatttcaaa atgccacagg tgtggaggac tgatcttgct ctcgacgtta aattaccctg
     2881 gaacctgatc ggaacctttg aagccattta ttccaaggct atcaatgatg tgatccagta
     2941 caatgccaac caggcgattg ccgactccat gttcttcggt gcagatgaca gggaacggtt
     3001 catgaaccgg aagatcaact caactgttag caatgccatg gtgctgacca atacaaaaga
     3061 agggtattcc tattcgttaa cggctcaatt ggccagagaa tttgaaaatg gcttctatgg
     3121 tttcctggcg tacacctatt ctgctacgaa ggatctgacc tcgaatccgg gatcggcagc
     3181 cgcttcggca tgggccagca atccgaccta taccaaccag aatgccccga ccctgagtta
     3241 ttcgcagttc agtgttcctc accgcgtggt tggttgtgta tcgtatggca ttgaatacgc
     3301 caaacatttg aaaacaacgg tatcgttgtt ctaccagggc agcagccagg gacgcctgga
     3361 ttatatctat tccaatgata tgaacggtga tggtaactct gccgatctta tgtacattcc
     3421 taaggatgag agcgaaatca tttttgccga cattaccaat ggcgacggcg aggtgattgc
     3481 aaccgcggca gagcaaggtg cggcattctg gcagtatgtc gaccaggaca aatacttgtc
     3541 tgaccataaa ggggaatatg ctgaaagata tggtgttgtg atgccctggg tgggtagatt
     3601 cgatgtgaaa attttacagg atgtatttac taaaatcggt aaaacaaaac acaccttaca
     3661 attcagttta gacttcctga acatcggcaa cctgattaat tcggactggg gtatttatcg
     3721 caaacaaacc ctgggtaatt atgatatcac cttactgaaa tacaaatcat ccaattcgga
     3781 aggagttccg acttatcagc tgaattatac gaaaactgat tcggatggca agcctgtttt
     3841 cccgacatct acctattctc cggtattagg tactaccagt acatggggaa tgcagattgg
     3901 tatcaggtac atcttttaac taatctctaa ataactcccg gaggctgtct caatcatttg
     3961 agcagcctct ttttttttaa attaaggatg tttaaatgat gaaaaagttt ctttatttgt
     4021 tgaccttttg cattagtttt gtttaaccac aaaggaacac aaagggtttc actaaggaca
     4081 caaagggttt cacaaagggc acaaaggatt tcacaaggga cacaaagatt cttaagccgg
     4141 ataatttagg agattatgta aattaatgta ttgaacatga cgtataaaaa ctataaactc
     4201 ttcctggtca ttgccttttt gagtctgaga accttcatct cggcgcagga aaagccatat
     4261 gtcgtgatgc tgtcgatgga cgggttccgg tgggattacc cggaaaggtg cggaacgccg
     4321 aacctggaca agatagccgc agagggcgtc agggcagaat cgcttcagcc tgccttcccg
     4381 tcaaagacat ttgccaacca ttatacgatg gccaca
//
LOCUS       DAOPNH010000277         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23271_length_4406_cov_4.574581, whole genome shotgun sequence.
ACCESSION   DAOPNH010000277 DAOPNH010000000
VERSION     DAOPNH010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23271_length_4406_cov_4.574581"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKZ74_12440"
     CDS             <1..437
                     /locus_tag="PKZ74_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93608.1"
                     /translation="SPCADYASVLLYSIINGEHTWPGADILIGVTNQDIEASNEIWNF
                     FTRFTLQGPVSIDENEPFAVISLDIFPNPVEGLATVDISGLESDGFIFRLMDINGRVC
                     KEQNNFREKRFLLDASLLPAGLYLAQIVQDRNIVVSTKLMIR"
     gene            complement(438..1463)
                     /locus_tag="PKZ74_12445"
     CDS             complement(438..1463)
                     /locus_tag="PKZ74_12445"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPM93609.1"
                     /translation="MSGVIDLRSDTVTRPSPAMLEYMFNAEVGDDVLGDDPTVIKLES
                     RIAGMFGKEDALFCPSGTMTNQIAVKVHTQPGDEVICESTSHVYRFEAGGMAFNSGCS
                     VRLIQGDRGRLKPHQVLENLNPDNVHYPQTRLVVAENTSNMGGGSIYDYADLVELSAT
                     CKEKNLRFHLDGARIFNALVETGQKPEDYGRLFDSVSVCFSKGLGAPVGSALIGSREF
                     ITKARRIRKVFGGGMRQSGYLAAAAIFALENNITRLKEDHRRAKVLSGHLAGMSYVEE
                     VYPAETNIIIFKLNSKYTQPQLLRLFEDRSLWAVGFGHDKIRLVTHFDFDDQLLARSL
                     EVFSSID"
     gene            1532..2104
                     /locus_tag="PKZ74_12450"
     CDS             1532..2104
                     /locus_tag="PKZ74_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93610.1"
                     /translation="MKSGKSHLYVLAVIVLVITACYEEWKTIVVAKGNHSSNEISPML
                     FQTDRIDFYFIADSSWYYPPPVSPGWNKIRGLSHGHHQNNSSARLVYQCIGNTLLVVG
                     AYCYADGISPQQNPALKGIIDTIQPGIKYFCSIHRKDDQYIFKFEDKTWIGPAGKYLS
                     WGYLLNPYIGGEFTLEKDWVVKIKDEKISE"
     gene            2120..2566
                     /locus_tag="PKZ74_12455"
     CDS             2120..2566
                     /locus_tag="PKZ74_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93611.1"
                     /translation="MNLYPILIHLHSIFRWVVLILIVMAIIDAVIKLRAKYKPVVKDS
                     KWKLFAMIVLHVQFIIGLILYFISPKVIFDAASMKNSMQRFFLVEHIALMIIAIVLIT
                     VGHVKSKRAVDNIRKQKLVIVYYGIGLLLILLSIPWPFRNFGAGWL"
     gene            2711..4402
                     /locus_tag="PKZ74_12460"
     CDS             2711..4402
                     /locus_tag="PKZ74_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93612.1"
                     /translation="MKKLVILLIISVLTGTGYLGAQDQPIQPSIVKTGVYHGISPALR
                     DLPAITDAEFQQLVIKSEQKMLNKKLRERHYPYASTAQPQGPDPVWQDQMGKTPGMKA
                     PIMNFSGQTSPYYPPDANGSAGPNHYMQTINTVYTIYNKSTGALVAGPTNLNLLFSGV
                     TGAEYNDGDPICLYDEQADRWLVAEFSISGSNDYMLIAVSTTNDPTGTWHKYSFDVAD
                     MPDYEKFGIWQDGYYMGTNNSTGNDIYVFERSKMLDGLTAQMVGFNNPWRPTTIDGFM
                     CVPPVDNDGTFAPAGSPGLFITINDDAIGGGTDQLWIYELAVNWTTPSNSTFTRSQQI
                     NVPAFDSNFGSNWDNIKQPNTTRELDAIPMVIMNVPQYRNFGSYQTIVCCHTVDVDNT
                     DHAGIRWYELRRTTGTWTVRQSGTYAPDANSRWMGSIALNGNNQIGLGYSISSSTVYP
                     GIRYCGQSASAYTSGNGVLDIAEEVIQNATSSQTSYNRWGDYAGICVDPSDNNTFWFT
                     TQYGGSRQTRIASFAFSNPPLTADFSGTPTIACTGSQVTFTDLNGRGCFMSVRPG"
BASE COUNT         1194 a         1077 c          986 g         1149 t
ORIGIN      
        1 attcaccttg cgccgattat gccagcgtac tgctttacag cataataaac ggagaacaca
       61 cctggccggg agcagatata ctaataggtg taactaacca ggacatagaa gccagcaatg
      121 agatatggaa ctttttcacc cggtttaccc ttcaaggacc agtgagtatc gatgaaaatg
      181 aaccctttgc ggtaatcagc ctggatattt ttcctaatcc tgtggaaggc ctggctacgg
      241 ttgatatttc cggtttggag tctgatgggt tcattttcag gctgatggat ataaatggga
      301 gagtctgcaa ggagcagaac aatttccgtg aaaaaagatt cttgctggat gcttccttac
      361 ttcctgccgg attatatctg gctcaaattg tgcaagatag aaatattgtt gtcagcacca
      421 agctgatgat caggtaatca atctatactg ctgaaaacct ccaggcttcg ggcgagcaat
      481 tgatcatcga agtcgaagtg tgttaccagc cgtatcttgt catggccgaa acccacagcc
      541 catagcgacc ggtcttcaaa caaacgcagc aactggggct gtgtgtattt gctgtttagc
      601 ttgaaaatga tgatgttcgt ttcggcagga taaacttctt caacataact cataccggcc
      661 agatgccccg aaagtacctt agcgcgcctg tgatcctctt ttaaccgggt aatattgttt
      721 tccagtgcaa atattgccgc ggcggccagg tagcccgatt gcctcatccc gccaccgaat
      781 acttttctga ttctcctcgc ttttgtgatg aattccctgc ttccgattaa agccgatccg
      841 accggtgccc ccaatccctt tgaaaaacaa acagaaacag aatcaaataa tcttccgtaa
      901 tcttccggct tctgacccgt ctccaccaaa gcattgaata tccgggctcc gtcgaggtga
      961 aacctgaggt tcttttcttt gcaagttgcg ctaagttcaa ccaggtcagc gtaatcgtaa
     1021 atgctcccgc cgcccatgtt ggatgtgttt tcagcaacta ccagccgggt ttgaggataa
     1081 tggacattat cagggttcag gttttccaat acctgatggg gtttgagccg accgcggtca
     1141 ccctggatca gtcgtacgga acaacctgaa ttaaaggcca ttcctccagc ctcaaaccgg
     1201 tatacatgtg aagttgattc gcagatcact tcatcgcccg gttgtgtgtg gactttaact
     1261 gcaatttgat tggtcatggt gccggaggga caaaaaagcg catcttcttt gccaaacata
     1321 ccggcaatcc gtgattccag cttaataacc gtcggatcat cgcccaggac atcatctccg
     1381 acttccgcat tgaacatgta ttcaagcatg gcaggtgagg ggcgcgttac agtatcgctt
     1441 cgtaaatcta ttacgccaga catattatct ttgtttaaaa tcaggttata aaattataaa
     1501 tgtatgatga ggtaatacca aattaatcga aatgaaatca ggaaaaagcc atttatatgt
     1561 attagcggta atcgttttag taataacagc ctgttacgaa gaatggaaga ccattgttgt
     1621 cgctaaagga aaccactctt ccaatgaaat cagcccgatg ctatttcaaa ccgacagaat
     1681 tgacttttat tttatagccg attccagttg gtattatccg ccacctgttt ctccaggatg
     1741 gaataaaata cgtggtttga gccatggtca tcatcagaat aactcttcgg caaggcttgt
     1801 ataccaatgt atcggcaaca cgctgctggt tgtcggcgca tattgttatg ctgatggaat
     1861 cagcccgcag cagaatcctg cacttaaagg aataatcgac acaattcagc ctggaattaa
     1921 gtacttttgc agtatacaca gaaaggacga tcaatacata tttaagtttg aagataaaac
     1981 ctggattggc ccggctggaa agtatttatc atggggctac ctgctgaatc catatatcgg
     2041 cggggaattt acgctggaaa aagactgggt tgtgaaaatc aaagatgaaa aaatcagtga
     2101 ataacgaaaa tctgaagcca tgaacctata tcccatactg atacatttgc acagcatttt
     2161 tcgctgggta gtgcttatat tgattgttat ggccataatt gatgcggtca taaagctgag
     2221 agcgaaatac aaacccgttg ttaaagacag caagtggaaa ttattcgcca tgatcgtcct
     2281 gcacgttcag tttatcatcg ggctgatact ctattttatt agtcctaaag tcatttttga
     2341 tgctgcctcc atgaaaaaca gcatgcagcg ttttttcctg gtcgaacaca ttgccctgat
     2401 gataatcgca attgtgttga taacagtagg gcatgtaaaa tccaaacgcg cggtcgacaa
     2461 tatcagaaag caaaagttgg tcatagtcta ttacggaatc ggtttgctat tgatcctgct
     2521 ttccattcca tggcctttcc ggaactttgg cgccggctgg ctatagaaat cttttttttg
     2581 cattctttcc ccggaaccga ataaccttta ccggtctttg gtgattaatc atgtcgtgtt
     2641 gcaaccatct gcagggtgca ccgtaaaagt ttaaatcact tatgcaataa ccttaaacca
     2701 aaaatcaatt atgaaaaagc ttgtaatttt attaattatc agcgttttga caggcacagg
     2761 ttatcttggg gcacaagacc aacccatcca gccatccatc gtgaaaaccg gtgtttacca
     2821 tgggatttcg cctgctttgc gggatcttcc ggctataaca gatgcagagt ttcagcagct
     2881 tgtaattaag agcgagcaga aaatgttgaa caaaaaactt cgcgagcggc attacccata
     2941 tgcgagcacc gctcagccac aggggcctga tccggtctgg caggaccaga tgggcaaaac
     3001 gcctggaatg aaagccccga tcatgaattt cagtggtcag acgtcgccct attatccgcc
     3061 tgatgccaat ggctcagccg gaccgaacca ttacatgcag acgatcaata ctgtgtatac
     3121 catatataat aaatctaccg gagccctggt tgccgggccc accaatctga acctgctttt
     3181 cagcggcgtg accggtgcgg aatataatga cggggatccg atctgtttgt atgacgaaca
     3241 agccgaccgc tggctggttg ccgagttctc catcagcgga tccaacgact atatgctgat
     3301 cgccgtttct accaccaacg acccgacagg gacctggcac aaatactctt tcgatgtcgc
     3361 cgatatgcca gactacgaga aattcggcat ctggcaggac ggttattaca tgggaaccaa
     3421 taacagtacc ggtaacgata tttatgtttt tgaaaggtcg aaaatgctgg atggactcac
     3481 cgcacaaatg gtcggtttta acaacccctg gaggcccacc acgattgacg gttttatgtg
     3541 cgttcctccg gtagataacg acggcacatt tgctccggcc ggctcacccg gattgttcat
     3601 caccattaat gacgatgcta tcggcggtgg taccgaccag ctctggattt acgaactggc
     3661 agtcaactgg accacaccat caaattccac attcacccgg agccagcaaa tcaatgtgcc
     3721 tgcattcgat agcaatttcg gcagcaactg ggataatatc aaacaaccta acacaacccg
     3781 tgaactcgac gccataccca tggtgatcat gaatgttccg caataccgga actttggatc
     3841 ctaccagacg atcgtttgct gccatacggt cgatgtcgac aataccgatc atgccggtat
     3901 tcgctggtat gaactccgcc ggacaactgg cacctggact gtcagacaat caggtaccta
     3961 tgcgccggat gcaaattccc ggtggatggg aagcatcgcc ctgaacggca ataaccagat
     4021 cggtctcgga tactccatct ccagttcaac cgtctatccg ggaatccgtt attgcggtca
     4081 gtcggccagc gcttacacct ccggaaacgg ggtgctcgat attgccgagg aggtcattca
     4141 aaatgcaacc tcttcacaaa catcctacaa ccgttggggc gactatgccg gtatctgcgt
     4201 tgatccctcc gataataaca cattctggtt taccacacag tatggcggaa gccgtcaaac
     4261 gcgcatcgca tcgtttgcat tctccaaccc gcctttgaca gccgacttta gcggcacacc
     4321 gacaatagct tgcaccggaa gccaggttac cttcaccgat ctcaatggcc gcggctgctt
     4381 catgtccgtc cggcctggat gatcct
//
LOCUS       DAOPNH010000278         4327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_23881_length_4327_cov_7.408942, whole genome shotgun sequence.
ACCESSION   DAOPNH010000278 DAOPNH010000000
VERSION     DAOPNH010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23881_length_4327_cov_7.408942"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PKZ74_12465"
     CDS             <1..881
                     /locus_tag="PKZ74_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPM93613.1"
                     /translation="LNRQGIENTWCDIRTVVRTDSVFRDASIDWEVSEKQSDAILKPL
                     LKSVGGIRPVVVTQGFIGSDDLGRSTSLGREGSDYTASVLASLLDASEVITWKDVPGI
                     LNADPRHFSQTIKLERISYSEATELAYYGAKVLHPKTIKPLQNKNIPLQVRSFEEPDQ
                     PGTLIQEETGVESLVPSFIFKFGQVLMSVSAKDFSFIDEKVFRDVFSLLSKYAIHVNI
                     IQNSALSLSFCTDSHGHLVELIDGLRDDYIVKYNEGLELITIRHSNDRASEIVLSGRE
                     VLLEQHNRVVSQYVVR"
     gene            941..2158
                     /locus_tag="PKZ74_12470"
     CDS             941..2158
                     /locus_tag="PKZ74_12470"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM93614.1"
                     /translation="MKESIVILAGGGPAPGINTVISSIAKVFLKDGYRVIGMHEGYKT
                     LFTSNPKTQDFDFELADKIKNQGGSALVMSRHKPKDSEFNTDFFVANNVKLLVTIGGD
                     DTASTANRISQFLSNHDIKIQNIHVPKTIDNDLPLPEGQPTFGYQSAKEEGVRIASTI
                     YEDSRTSGNWFIISAMGREAGHLAFGIGGACHLPMIIIPEMFNKTDISFDKITRMIIS
                     SILKRKILGISYGVAIISEGVFHFMTDEEIESTNISFTYDDHGHPELGNVSKAHIFNL
                     LLQRKLKELNISVKSRPEELGYELRCISPTAFDLMYCSLLGLGVKILYDQGMSACMVT
                     SDPKGDVYPLFLKDVTDEKGKVKPRMVNINSQKVTMVYEYNLQFLTEEDYEAARKYVP
                     DPENYDFRKILNW"
     gene            complement(2162..3238)
                     /locus_tag="PKZ74_12475"
     CDS             complement(2162..3238)
                     /locus_tag="PKZ74_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93615.1"
                     /translation="MKIKIQFLLSFFILVMMNVIFGQAPRTLDEDINRLAGKIVQYPA
                     REKYLTEIKSKYDEACRIDRERIQELRAGGQPDIWYEIFRTYQVLENRQAVVKRIPDA
                     SLQKTGIVIADYSKEMDEAKYKVTAYLYAHALKLLETGSVPDSRQAYAELMKAASLNN
                     SYKEIDKLIRKAVLKGASDVEFEMDNRTGKVVSSSMIRYLSVIVWEFKKANYGHEKPG
                     KPDKSFPFTLRVVLDELQIGKDQVRELQYQEERDVMRDFMIVDTIRCQISETRQLKKA
                     VLSGRLEYIDRLTGKVVNRVPVKVESIFTNAYATLQGNPDAAGDETRRLLKSSRVAYP
                     SNEQMILDATEEFAKKATEIILGE"
     gene            3362..>4327
                     /locus_tag="PKZ74_12480"
     CDS             3362..>4327
                     /locus_tag="PKZ74_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPM93616.1"
                     /translation="MAQLLVRFILRNRLANLLVILAMTGFMGYMATGLKISYDFQQML
                     PYTDSISIDYHHFKEVFGEDGSVMFIGIKSDNLFELEEFNDWWDLTYSIKAMEGVGEV
                     VSLARIYQLEKDDSLRKLNFDLVFQGKPQSQAKLDSLKNVVLSIPFYEGFLINKETGA
                     TLMMVTLNKEEIKNRNRVRLVKDIVDLTDKFGQNHNIKVHYSGLPYIRIKIAEKLQKE
                     QFMFVLSALALTVLILLAFFRSFKIALFTLIIVLINVAWILGLNVVLGYKLTILTAIL
                     PPLLIVIVVENCIFMLTKFHHEFKLHRNKVKALSRMIMRLGTTNLL"
BASE COUNT         1099 a         1069 c         1053 g         1106 t
ORIGIN      
        1 atttaaaccg gcagggtatt gaaaacacgt ggtgcgatat ccggaccgta gttcgcaccg
       61 attctgtttt ccgcgacgct tcgatcgact gggaagtgag tgaaaagcag tcggatgcca
      121 ttttgaaacc gctgctaaag agcgttggag gcatccggcc ggttgtcgtg acacaggggt
      181 ttatcggcag cgatgacctg gggcggagca catcgctggg ccgggaaggg tcggattata
      241 ccgcttcggt gctggcctcc ctgctcgatg ccagtgaggt gatcacctgg aaagatgtgc
      301 ccggcatcct gaatgccgat cccagacatt tcagccagac catcaagctg gagcggattt
      361 cttacagcga ggcgacagag ctggcctatt acggcgccaa agtccttcat cctaaaacaa
      421 taaaaccgtt acaaaacaaa aacatacctc tccaggtcag gtcgttcgaa gaacccgacc
      481 agcccggcac cctgatacag gaagagaccg gcgtcgaatc gctggtcccc agttttatct
      541 tcaaattcgg acaggtgctg atgagtgttt ccgcaaaaga tttttctttt atcgatgaaa
      601 aagtattccg ggatgttttc tcccttttat cgaaatatgc catccatgtg aatattattc
      661 agaactcggc cctgagcctt tcgttctgca ccgacagcca cgggcacctg gttgaattga
      721 ttgacggcct tcgtgacgat tacatcgtaa aatacaacga ggggctggag ctgatcacca
      781 tccgccattc gaacgacagg gcatcggaaa tagttctgag cggccgggag gtactgctgg
      841 aacaacataa ccgggtggtt tcgcaatatg tagtcagata gggccgcgtc tcgcaaatct
      901 tgctattttt gcaccttcaa acacaaactt tcccacgtat atgaaagaat ccattgtcat
      961 tttagccggc ggcgggcccg cacccggcat caacaccgtt atcagcagca ttgccaaggt
     1021 ttttctgaaa gacggttacc gtgtcatcgg gatgcatgaa ggatataaaa ctttgttcac
     1081 gtccaatccg aagacacagg attttgattt tgaactggcc gataagatca aaaaccaggg
     1141 tggttcggcg ctggtcatga gccgtcacaa gccgaaagac agcgaattca acactgattt
     1201 ttttgtggcc aacaatgtaa aactgctggt caccattggc ggggatgata cagcttcgac
     1261 ggccaacagg atctcacagt tcctgtcgaa tcacgacatc aaaattcaga acatccatgt
     1321 tccaaagacg atcgataacg atctgccatt accggaaggg cagccgacat tcggttacca
     1381 gtcggccaag gaggagggcg tccgtatcgc atcgaccata tatgaggatt cccggaccag
     1441 cggtaactgg ttcatcatct cagccatggg acgcgaagcg gggcatctcg ctttcgggat
     1501 cggcggcgca tgccatcttc ccatgatcat tatccctgag atgttcaaca aaaccgatat
     1561 ttccttcgat aaaatcaccc ggatgatcat ctcttccatc ctgaaaagaa aaatcctggg
     1621 gatcagttat ggcgttgcca ttatcagcga aggcgttttc catttcatga ccgatgagga
     1681 gatcgagtcg accaatatca gcttcactta cgacgatcac ggccacccgg aactgggcaa
     1741 tgtcagcaag gcgcatatct ttaacctgct gttgcaaaga aagttaaagg aactgaatat
     1801 ctcggttaaa agccgcccgg aagagctggg gtacgaactc cgctgtatca gcccgaccgc
     1861 tttcgacctg atgtactgct cattgctcgg gcttggcgta aagatcctct acgaccaggg
     1921 gatgtcggcc tgtatggtca cttccgatcc caaaggggac gtatatccgc ttttcctgaa
     1981 agatgtcacg gatgaaaagg gcaaagtaaa accgcgaatg gtcaacatca attcacagaa
     2041 agtgaccatg gtttacgaat acaacctcca attccttacc gaagaggatt acgaagcggc
     2101 ccggaaatac gtacccgatc ctgaaaatta cgatttcagg aaaatcctga actggtagga
     2161 tttactcacc caggataatt tctgtcgctt tctttgcaaa ttcctccgtt gcgtcgagga
     2221 tcatttgttc attggaagga taagccaccc gtgaagattt cagcaggcgg cgggtttcat
     2281 cgccggctgc atcgggattt ccctgcaggg tagcatacgc attggtaaag atcgattcca
     2341 ccttcaccgg cacccggttg accaccttgc ccgtcagccg gtcgatgtat tccagcctcc
     2401 cggataatac cgctttcttc aactgacggg tttcggaaat ctggcaccgg atcgtatcga
     2461 ctatcataaa atcgcgcatc acatctctct cctcctggta ctggagttcc ctgacctggt
     2521 ctttgccgat ctgcagctcg tcgagaacta cccggagggt aaaagggaaa cttttatcgg
     2581 gcttccccgg tttttcatgg ccgtaattcg ctttcttgaa ctcccagacg atgacggaga
     2641 ggtaacggat catggaagag ctgaccactt tgcctgtacg gttatccatt tcgaattcca
     2701 catcggaagc gcctttgaga acggctttcc ggatcagttt atcgatctct ttataggaat
     2761 tgttcagaga agcggctttc atcagttcgg cgtatgcctg ccgtgaatcg ggtacgctgc
     2821 ctgtttcgag cagtttcagc gcatgggcat aaagataggc cgttaccttg tatttggcct
     2881 catccatctc cttggaataa tcggcaatta caattccggt tttttggagc gaagcgtcag
     2941 ggatcctttt aactaccgcc tgcctgtttt ccagtacctg gtacgtcctg aagatttcat
     3001 accagatatc cggctggccg ccggcacgca gttcctggat ccgttcccgg tcgatcctgc
     3061 aggcttcatc atattttgac ttaatctccg tcaaatactt ttccctggcg ggatactgta
     3121 caattttgcc cgccaaccgg ttaatatctt cgtccagcgt ccggggagcc tgtccgaata
     3181 ttacgttcat catgaccaat ataaagaatg ataacaggaa ctggattttt attttcatga
     3241 ccggtaattt tttcgttgtg tgtcctaatt aaaattaaat ttgaacccgc gataaagcag
     3301 agactttcaa ttgcaccaaa ttttgtgcaa aactattata aagtaaaaat atttttcttt
     3361 catggcccag ttactggtcc ggttcattct tagaaacagg cttgccaacc tgctcgtaat
     3421 cttagcgatg acaggtttta tggggtatat ggcgaccggg ttgaaaatat cctatgattt
     3481 tcagcagatg ttgccataca ccgatagcat cagcatcgac taccatcatt tcaaagaagt
     3541 tttcggagaa gatggttcgg tgatgttcat cgggataaaa agtgacaatc tttttgagct
     3601 ggaagaattc aacgactggt gggacctcac ctattctatt aaggcgatgg agggggttgg
     3661 agaggttgta tctctggcca ggatatacca gctggaaaaa gacgacagtc tgcggaagct
     3721 gaatttcgac ctggtcttcc agggaaaacc gcaaagccag gccaaactcg acagccttaa
     3781 gaatgtcgtg ctttcaatcc ctttttacga aggtttcctg ataaataaag agaccggggc
     3841 caccctgatg atggtcacgc tgaacaaaga ggagatcaaa aacaggaacc gggtcaggct
     3901 ggtcaaagat atcgtggatt taaccgataa gttcgggcag aaccacaaca tcaaggtaca
     3961 ctattcggga ttaccctata tccggatcaa gatcgccgag aagcttcaga aagagcagtt
     4021 tatgtttgtc ctctcggccc ttgccctgac cgtactgatc ctgctggctt ttttccggtc
     4081 gttcaagatc gcgctgttca cactgattat agtcctgatc aatgtagcct ggatcctggg
     4141 actgaatgtt gtcctggggt acaagctgac gatcctgacg gctattttac cgccattgct
     4201 gatcgtgatc gtcgtggaga actgcatctt catgctcacc aagtttcacc acgagtttaa
     4261 gttgcaccgg aataaggtaa aggcgctttc gcggatgatc atgcggctgg ggacgaccaa
     4321 cctgttg
//
LOCUS       DAOPNH010000279         4297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24135_length_4297_cov_3.964168, whole genome shotgun sequence.
ACCESSION   DAOPNH010000279 DAOPNH010000000
VERSION     DAOPNH010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24135_length_4297_cov_3.964168"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..2721)
                     /locus_tag="PKZ74_12485"
     CDS             complement(352..2721)
                     /locus_tag="PKZ74_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93617.1"
                     /translation="MKYYRLLFFTAALLIVLAGYGQTEEEINTPIVSKAKYFDTTPPL
                     RDMPIVLPGERDRSWKDGIIQNKSMEDEYKNREADIPEGFVDPVLQKNYSANREISGP
                     VVNMDGIGNLCSCYPPDTDGDVGPNHYFQMINLSFAIWDKQGNLLYGPVDNSTLWDGF
                     IGPWTGTNDGDPIVLYDEMADRWMASQFAVNTNNGSYWQLIAVSTTPDPTGPYHRYAF
                     QMVAFNDYPKLSVWPDGYYATWNMFGSYTRVGVAAFERDAMLAGDPDAQMVYFNQPSG
                     TFSMLPADVDGTPPPEDAPCYFGYIRMFSTQNFEVYEFDVNWEFPNMSTFTMVNSMST
                     AAFNPNLDGIPQPNTGTTLDDLGMMMMFRLQYRNFGSYQTLVANHSVNAGSNRAGIRW
                     YELRKETDDWYIYQQGTYAPNDGQHRWMGSIAMNGNGDIALGYSLSSTTTYPSIYYVG
                     RRADAPLGEMNLDEVKIMSGTGSQSGINRWGDYSCLSVDPVDDTTFWFTTEYNRNGWK
                     TRIASFDFGPILPPTIDAGPNDTICEDVLFLADATASYQQSVLWQSSGDGFFPDPNVV
                     DVTYLRGNGDLANGQVTLWATVTGYIEGQTDTDSVTVYFTKTSKAFAGPDTTICDDDV
                     IQLSGSAIFQDSIYWTTEGDGTFDDPGIFTPVYTPGSEDISKGYAWLRLTAYDSLPCD
                     NSHFDRVKITIDECTGISEPEMGKLGLRVVPNPATSRLNFEVTGMEAGTEAVLNLTNQ
                     QGAPVFTMKLPATVGSYSNSMNTVNFPRGVYYLKVTGLKEQAIQKVILQ"
     gene            complement(2793..3206)
                     /locus_tag="PKZ74_12490"
     CDS             complement(2793..3206)
                     /locus_tag="PKZ74_12490"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPM93618.1"
                     /translation="MASDTIIHPGVIDHIEGDKAFVKILSQSACGSCHAKGMCTVAEV
                     EEKIVEVGINSSFSHKEGDAVMVRMDESLGRKAVWMGYGLPFIILMSSIVIFLSVLNN
                     EGLAALLSIAMLAPYYLVLYLMRGRLHKEFRFRID"
     gene            complement(3246..>4297)
                     /locus_tag="PKZ74_12495"
     CDS             complement(3246..>4297)
                     /locus_tag="PKZ74_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM93619.1"
                     /translation="HGIPPSHPRDTLYDDSTYYWSKATLISHINSGRPWLHHVGHANQ
                     SYVMKLSLVDITNANFAQTNGVTHNYTVVYTHGCICGGFDYTDCIGERMIGIDNFASA
                     FVGNSRYGWFNQGTTDGPSQHLHREFMDALYYDSLYRIGMAHLKSKSETAPFVEIGNE
                     EFEPGATRWCFYDNNVLGDPVMALRTEEPQGIALVSETMVPASSASVAVQLESSTGNV
                     KGLSCALMKNDTLYGSALTDGSGLAVIYPDSVLAEGEYHLWITGYNILPQMIPIHACN
                     YWLGYSPDWGNPANWFTGHIPDSTSYIIIPSQPAGTKFPSFNSSTERHCRHFMIEENA
                     QFRIGPGETLKVWGD"
BASE COUNT         1103 a         1066 c         1049 g         1079 t
ORIGIN      
        1 gaccaatcgc actgagcgac acaacggcaa aaatcaatac actgttcact ttgttctctt
       61 tttaatactg ccttacgcat taagggctta aggccggttt aggatgagca aatgtaaaaa
      121 caattgtgaa aacagtaaca ataaggatcg aaaatcggat tgggtataaa atactgatta
      181 ttaatattct atcggataaa aatagaagct tcagaaatat tgttcctgaa gcttctgaaa
      241 atcacggggt tcctgtaaaa atttagaaac cgtctaaatt attgaatctt aatcattttt
      301 aagtcggatg atcctgcata ttgcccggtt tatccaatta ttattttctg tttactgaag
      361 aatgactttt tggatagcct gttctttcaa gccggttact ttcagataat aaacccctct
      421 cgggaaattc acggtgttca ttgaattgga ataactaccc acggtagcgg gcaatttcat
      481 ggtaaacaca ggtgcgcctt gctggtttgt caggtttaac acggcttcag taccggcctc
      541 cataccggtt acttcgaaat taagcctcga tgtggccggg ttcggtacca ccctgagccc
      601 cagtttaccc atttccggtt ctgaaatacc ggtacattca tcgatagtga tcttgaccct
      661 gtcgaagtgc gaattatcac agggcaatga atcataagca gttaacctca accaggcgta
      721 tccttttgat atatcttcgg atccgggcgt ataaaccggt gtaaaaatcc ccggatcatc
      781 gaatgtaccg tcgccttcgg tagtccaata gatggaatcc tggaaaatgg ccgaaccgga
      841 gagctggatg acatcgtcgt cgcaaatcgt cgtgtcgggg ccggcaaaag ccttggaagt
      901 ctttgtgaaa taaacggtta cagaatccgt atcggtctgg ccttcgatat aacctgtcac
      961 cgtagcccag agtgtcacct gtccgttggc aaggtcgcca ttacccctga gataggttac
     1021 atccacgaca ttcggatcgg gaaagaagcc atctccggac gactgccaca ggacgctctg
     1081 ctggtaggat gcagtggcat cggcaagaaa caggacatct tcacaaatgg tgtcattggg
     1141 cccggcgtca atagttggtg gcagaatcgg gccaaagtca aaggaggcga tccgggtttt
     1201 ccatccattc cggttatatt ccgttgtaaa ccaaaaggtc gtatcatcga ccgggtcgac
     1261 cgacaaacaa ctgtaatcgc cccagcgatt gatcccgctc tgggatccgg tacccgacat
     1321 aatcttgacc tcatccaggt tcatctcgcc cagtggagca tcagctcttc ttcctacata
     1381 ataaatggag ggataagtag tagtgctcga cagactgtaa cccagcgcaa tatcgccatt
     1441 gccattcatg gcaatacttc ccatccagcg gtgctggccg tcgttcgggg cgtaggtccc
     1501 ctgctggtaa atgtaccagt cgtcggtctc ttttctcagc tcataccatc ggatcccggc
     1561 cctgttgctg cccgcattca ccgaatggtt ggccaccagc gtctggtagg aaccaaagtt
     1621 cctgtactgc aggcggaaca tcatcatcat acccaggtca tccagtgtcg taccggtgtt
     1681 aggttgcggg atcccgtcaa gatttggatt gaaagcggct gttgacatgg aattgaccat
     1741 ggtaaaagtc gacatgttcg ggaattccca gttgacatcg aattcataca cttcaaagtt
     1801 ctgggttgaa aacatccgga tatagccaaa atagcaggga gcatcttccg gtggcggggt
     1861 tccgtcgacg tcggccggga gcatgctgaa ggtcccggaa ggctggttaa aatagaccat
     1921 ctgggcatcg ggatcaccgg ccagcatggc atcacgctca aatgcggcaa caccaacgcg
     1981 ggtatagctt ccgaacatgt tccaggtggc gtaatagccg tcgggccaaa ccgaaagttt
     2041 cggataatcg ttaaaggcta ccatctggaa agcatagcgg tgataaggcc cggtggggtc
     2101 gggtgtggta ctgacggcaa tcagctgcca gtaggaaccg ttattggtat ttacggcaaa
     2161 ctgcgaagcc atccagcggt cggccatttc atcgtaaaga acgataggat ctccgtcgtt
     2221 cgtgccggtc caggggccga taaagccatc ccacaacgtg ctgttgtcga ccgggccata
     2281 gagcaggttc ccctgtttat cccagatggc aaacgaaaga ttgatcatct ggaaataatg
     2341 gttaggcccc acatccccgt cggtgtcggg aggataacaa ctgcagaggt tcccgatccc
     2401 gtccatattg acaacaggcc cgctgatttc gcggtttgca ctgtaatttt tctgtaaaac
     2461 aggatcgaca aatccttccg gaatatcagc ttccctgttt ttatattcgt cctccattga
     2521 tttattctgt atgatgccgt ctttccatga acggtcgcgt tcacccggca ggacaatcgg
     2581 catatccctc aacggagggg ttgtgtcgaa atacttcgcc ttcgacacga ttggcgtatt
     2641 tatttcttct tctgtctggc cgtagcctgc cagaacaatt aacaaagcgg cagtaaaaaa
     2701 cagtaatcgg taatacttca tgacacttct ccggttaaat tattagttag ataaaatagt
     2761 tcttaaacaa caatattgca tcaatgttca ttttaatcaa tcctgaatct gaactccttg
     2821 tgtaatcttc cgcgcatcag gtataatacc agataataag gggccagcat ggcaatcgac
     2881 agcaatgcgg caaggccttc attattcaat accgacagga atatcacaat ggacgacatc
     2941 aggataatga acggaaggcc atagcccatc cagactgctt tccgcccgag cgattcatcc
     3001 atcctcacca tcaccgcgtc cccctcttta tgagagaaag aagaatttat cccgacttcc
     3061 acaattttct cttcgacttc ggctacagtg cacatccctt tggcatggca ggaaccgcat
     3121 gccgactgtg acagtatttt cacaaaagct ttatcgcctt cgatatggtc aataacaccc
     3181 gggtggatga tcgtgtcgga agccatgatt ctattccgtt aagcggctgc aaagataacg
     3241 gaaaatcaat ctccccagac tttcaaagtt tcaccggggc ctatcctgaa ctgtgcgttc
     3301 tcttctatca tgaaatgacg gcagtgtcgc tctgtgctcg agttaaacga ggggaatttc
     3361 gtcccggccg gttgggatgg aataataata tacgacgtac tatccggtat gtgcccggtg
     3421 aaccagtttg caggattgcc ccagtctggt gaatagccga gccagtaatt gcaggcatgg
     3481 ataggaatca tctggggcag aatgttatag ccggtaatcc agagatggta ttcgccctcg
     3541 gccagcaccg agtcgggata gataaccgcc aggcccgatc catcggtcag tgcgctgcca
     3601 taaagcgtgt cgtttttcat cagcgcacag gaaagccctt tgacatttcc ggttgaagat
     3661 tccagttgca ctgcgactga agcggaagaa gccggcacca tggtctcgct gaccagtgct
     3721 ataccctgtg gttcttcggt acggagcgcc atcaccggat cgccgagcac attattgtcg
     3781 taaaaacacc agcgcgtagc tccgggctcg aactcttcat tgccgatctc cacaaatggc
     3841 gctgtttcgc tcttggattt caaatgggcc atgccgatcc ggtaaaggct gtcgtaatac
     3901 aaagcgtcca tgaattcccg gtgaagatgc tgcgaagggc cgtcggtggt accctggtta
     3961 aaccagccgt acctggagtt accgacaaaa gcggaggcga aattatcgat cccgatcatc
     4021 ctttcaccaa tacaatcggt ataatcaaac ccaccgcaaa tacaaccgtg ggtgtaaacg
     4081 accgtgtaat tatgggttac cccgttggtc tgtgcgaagt tagcattggt aatatcaacc
     4141 agggaaagct tcataacata cgactggttg gcatgcccca cgtggtgcag ccagggtctt
     4201 ccactgttga tgtggctgat gagggtggct ttggaccaat aataagtcga gtcgtcgtaa
     4261 agcgtgtcgc gcggatggga tggcgggatg ccgtgcg
//
LOCUS       DAOPNH010000280         4249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24518_length_4249_cov_5.305675, whole genome shotgun sequence.
ACCESSION   DAOPNH010000280 DAOPNH010000000
VERSION     DAOPNH010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24518_length_4249_cov_5.305675"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PKZ74_12500"
     CDS             complement(<1..1092)
                     /locus_tag="PKZ74_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM93620.1"
                     /translation="MTEMIQNTLRESKVARWTALGLVAFTMLAAYFFVDMMAPLQSLL
                     EKKYTWQPDTYGFFSGSEYMLNVLGFLIISGIILDKMGIRFTGMTAATTMLVGGLIKY
                     YGLTDYFNHGGFGYEFFNSFWTSMPASAKVASIGYAIFGIGVEMAGITVSKTIVKWFR
                     GKELALAMGMEMATARLGASVAFFFSSAIAGAGKPGEDPIKSIWFGLGLLAIGFLTFL
                     TYIFMDAKLDKQDNTNKSEEPAEEFRLSDLGKILTNRGFLLISLLCVLFYCGVFPFLK
                     YAVNMMQNRLGISNLAAGYISGLLPVGTIILTPIIGNFLDRKGKGASLMIYGAALLTI
                     AHLTFALAPLNMALAIIAIMVLGVAFSLVP"
     gene            complement(1159..1854)
                     /gene="rsmG"
                     /locus_tag="PKZ74_12505"
     CDS             complement(1159..1854)
                     /gene="rsmG"
                     /locus_tag="PKZ74_12505"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPM93621.1"
                     /translation="MNFELINFHFPSLTAGQLDQFRQLGILYPEWNAKINVISRQDIG
                     NLYERHILHSLGIAKVITFKTGTRILDAGTGGGFPGIPLAILFPEVYFHLVDSTAKKL
                     MVVREIASAAGITNVTVQHQRLEEHLNEYDFVVSRAVASLDQMVDWVFKNIKKDGFND
                     MINGILYLKGMETGEEYNNRTIEQLNNRTIEQLNNRSGEVAGRRRIEYRNYPLSDYFA
                     APFFATKAVVHLF"
     gene            2247..3212
                     /locus_tag="PKZ74_12510"
     CDS             2247..3212
                     /locus_tag="PKZ74_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPM93622.1"
                     /translation="MKLRSIFLCITCSGIVSLTYSQERITLAQYIEQYKDLAIEEMET
                     YRIPASITLAQGILESDCGNSPLAREANNHFGIKCHKGWTGATYHQDDDAPNECFRKY
                     RKAEESYRDHSLFLTTRDRYKLLFDLEVTDYKGWAHGLKQAGYATNPRYPELLIKIIE
                     ENRLDAYDRADSRQQPLISRNNASDTSNTTFNLQPSTLPPDNFPIAGRGENDRIIFKN
                     NGVKFVLARKGDTYRKIAGEFGIYTWQVEKYNEAGKKSKLKAGDKVYLEKKKGSAEAK
                     SHVVRAGETIRSVSQDYGVRLKALCRKNRIKDKDKIREGEVLLLK"
     gene            3470..>4249
                     /locus_tag="PKZ74_12515"
     CDS             3470..>4249
                     /locus_tag="PKZ74_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93623.1"
                     /translation="MKRIFFTFLILVAFLAGYSQMTNNGGTITVENGATLVIEGDYTS
                     TNSGSIVIDGAVQLKGDFVNNGGSIDAASAGSITFNGTAPQEITGNTPTNFYCAVTVN
                     NTNGVALTNTQTGASQTMSNTFTLSGGKVTLNNFDLTVGSTGVTGADASKYIVTNGTG
                     QLKASVANTNFLMPVGSATYYNPVILNEAGTSDVYGVIFSEGKPGGWTGTDHAVDGYW
                     TVTEGLAGGANLSVTPQWAGAQEDANFDNQDCAVGVSSDLGG"
BASE COUNT         1194 a         1005 c         1045 g         1005 t
ORIGIN      
        1 cggaaccagc gaaaaagcca cgccgagtac catgatggcg atgatggcca gggccatgtt
       61 cagcggcgcc agtgcaaagg taaggtgggc aatggtcaaa agggcagcgc catatatcat
      121 caggctcgct ccttttcctt ttctgtcaag aaaattacct ataattggcg taaggattat
      181 ggttcccaca ggcaaaaggc cactaatgta accggcagcc aggttgctga tcccaagcct
      241 gttctgcatc atgttgacag catatttgag gaaggggaaa acaccgcaat agaacaggac
      301 acaaagcagg gagatcagga gaaatccgcg gttggtcagg attttcccaa ggtcgctcag
      361 gcggaactct tcggcaggtt cttccgactt attcgtatta tcctgcttgt cgagtttggc
      421 atccataaaa atgtatgtca ggaaagtcag gaacccgatg gccagcagtc ccagtccgaa
      481 ccagatcgac tttatcggat cttcgcccgg ttttccggcg cctgcgatgg cggaggagaa
      541 gaagaatgcc accgaagcac cgaggcgggc tgtcgccatc tccattccca tggccagggc
      601 cagctctttg cccctgaacc acttgacaat agtcttggat accgtgatcc cggccatttc
      661 cacaccgata ccgaaaatgg catatccgat ggaagccact tttgccgaag ccggcatact
      721 ggtccagaac gaattgaaga actcatagcc aaatccgccg tgattgaaat aatcggtcag
      781 gccgtagtat ttgatcagtc ctccgaccag cattgtggtg gctgccgtca taccggtaaa
      841 gcggataccc attttgtcga ggatgatccc ggaaatgatc aggaaaccga gaacgttcag
      901 catgtactcc gatccggaga agaatccgta agtgtcaggc tgccaggtgt atttcttttc
      961 aaggagcgat tgtaaaggcg ccatcatatc gacaaagaaa taggccgcca gcatggtaaa
     1021 agctaccaat cccagggcag tccagcgggc taccttggat tctctcaggg tgttttggat
     1081 catttcagtc atataggttt tgtttaggtt aaaaagacag gcgaagataa taaataatta
     1141 tatcgggaaa gatgacagct aaaaaaggtg aacaaccgcc ttagtcgcaa aaaacggcgc
     1201 tgcaaaatag tcgctgaggg gataattcct gtattcaatc cttctcctgc ccgccacttc
     1261 gcctgatcta ttgttcaatt gttcaattgt tctattgttc aattgttcaa ttgttctatt
     1321 gttatattct tctcccgtct ccatcccttt cagatacaga ataccgttta tcatatcatt
     1381 gaagccatct ttcttaatat ttttaaacac ccaatccacc atctgatcca gtgaagccac
     1441 ggccctgctg accacaaaat cgtattcatt cagatgctct tccagccgct gatgctgaac
     1501 cgtaacattg gtgatcccgg cagctgaggc gatctccctg acaaccatca gcttctttgc
     1561 cgtgctgtcg accaggtgaa aatagacttc ggggaataaa attgccagcg ggatacccgg
     1621 aaaacctcct ccggtgccgg catccaggat acgggtaccg gtcttgaagg tgatcacttt
     1681 ggcaattccc agggaatgaa ggatatgccg ttcatacaaa ttgccgatat cctgcctgga
     1741 aatgacgttg atcttcgcat tccattccgg gtataaaatt ccgagctgcc tgaactgatc
     1801 cagttgcccg gcagtcaggg aaggaaagtg gaaattgatc agttcaaagt tcaaagttca
     1861 aagttcaaag ttggtcacaa aggtcgaaaa aaatcttatc aggaaacttt tcctggaacc
     1921 gatgtgacac tcgtcggaag gaaaacccgg caggaagagg gtgtcgcaaa agtctgatat
     1981 ttcgatttga ttgccttaca acagttcgtg ctccaacgac taaagccgtt ggttccgaga
     2041 atattacaaa gatggaaccg acggcttcag tcgtcggaag agaatctttc aaggaaccga
     2101 cggcttcagt cgtcggactg ccggcccctc ctccatcgtc ggaatgccga tttcagcctt
     2161 ttaacacgac aatgtgaata aaacgatccg attgtcgctg cccggcggat agatcacggt
     2221 tacatttgca aattaggggt cgaaaaatga aactaagatc gatattttta tgtataacct
     2281 gttcaggaat agtatcgttg acctattccc aggaacggat tacactggcg caatatatcg
     2341 agcagtacaa agacctggca atagaagaaa tggaaaccta ccggataccg gcaagcatta
     2401 ccctggcgca gggcatcctg gagtcggact gcggaaacag cccgctggcc cgggaagcca
     2461 ataatcattt cgggatcaaa tgccacaagg ggtggaccgg ggccacctat caccaggatg
     2521 acgacgcccc gaacgagtgt ttccgcaaat accggaaagc agaagagtcg taccgcgacc
     2581 actccctgtt cctgaccacc cgcgaccggt acaagctcct gttcgacctt gaggtcaccg
     2641 attacaaagg ctgggcgcac ggtctcaaac aagcaggtta tgctaccaat ccgcggtacc
     2701 cggagctgct tataaaaatc atcgaggaga accggctgga tgcgtatgac agagccgata
     2761 gccgccaaca gccgttaatc agccggaaca atgcctcaga taccagcaat acaactttca
     2821 accttcaacc ttcaacatta ccccctgata attttcctat tgctggccgg ggtgaaaatg
     2881 atcggatcat ttttaaaaac aacggcgtga aattcgtcct ggccaggaag ggggatacgt
     2941 accggaagat tgccggagaa tttgggattt atacctggca ggtggaaaaa tacaatgagg
     3001 cagggaaaaa atcgaagctc aaggcggggg acaaagtata cctggaaaag aaaaagggat
     3061 ctgcggaagc aaagagccat gtcgtccggg ctggtgaaac aatacgttct gtttcacagg
     3121 attatggagt acggttaaaa gcattatgcc ggaagaacag gataaaggat aaagacaaga
     3181 tcagggaagg ggaggtgtta ttgttaaaat gaggttaaat tttgctattt ttagacaatt
     3241 gacggtaatt ttatttcgat tattattact tttgttatga tgagtgatta attttaaaaa
     3301 tatttcggga aaagccctga aatttaaatt tattattgtt ttggtaatta aatttgtttt
     3361 actttgcatt attggaaacc aagtctgttc aaaaatcggc tgtttaagca tgaagcaaca
     3421 caaatgatta tgaactaaga atttagagca ggaacaaaaa cttatcatta tgaaaagaat
     3481 attctttacc tttttaattc tggtggcttt tttagccggt tactcacaaa tgaccaacaa
     3541 tggcggcact attactgttg aaaatggtgc cacattggtt attgaaggcg actataccag
     3601 cactaattca gggagtatcg tgattgacgg ggcggtacaa ctgaaaggcg atttcgtcaa
     3661 taacggcggt agcatcgatg cggcgtctgc cggcagcatt actttcaacg gcacggcccc
     3721 gcaggagatc acgggcaaca caccaaccaa tttttactgt gctgttacgg taaacaacac
     3781 caacggtgta gcgctgacca acacccagac cggggccagc cagacgatga gcaatacctt
     3841 caccctttcc ggagggaagg tgacgctcaa caattttgat cttacagtgg gcagcaccgg
     3901 ggtaaccggg gcagatgcca gtaaatatat tgttacaaat ggtaccgggc agcttaaagc
     3961 ttcggtggcc aacaccaact tcctgatgcc cgtcggatcc gccacctatt acaacccggt
     4021 gatcctgaat gaagcaggca catcggatgt ttacggtgtg atattctcgg aagggaaacc
     4081 cggtggatgg accggcaccg accatgccgt cgacggctac tggacggtaa cggaaggact
     4141 ggcaggtggc gctaacctct ctgtgacacc gcagtgggcc ggcgctcagg aagatgccaa
     4201 cttcgacaac caggattgcg ccgttggcgt ctcctccgac ctcggtggc
//
LOCUS       DAOPNH010000281         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24571_length_4242_cov_6.161691, whole genome shotgun sequence.
ACCESSION   DAOPNH010000281 DAOPNH010000000
VERSION     DAOPNH010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24571_length_4242_cov_6.161691"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKZ74_12520"
     CDS             <1..212
                     /locus_tag="PKZ74_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM93624.1"
                     /translation="RNFYGIINEYRIKEAKDLIMDQENSQIKIEVLGEMVGFQSKSSF
                     NACFKKMTGMTPSEYRHKHKNNFSK"
     gene            complement(204..896)
                     /locus_tag="PKZ74_12525"
     CDS             complement(204..896)
                     /locus_tag="PKZ74_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPM93625.1"
                     /translation="MIQLLTGFLAAAVHVVTGPDHLAAVTPLAIENRKKAWNIGLMWG
                     IGHVAGMLLIGLLYLAFKSMINVEKISGYSEFLVGFVLIGIGAWAIVKTLGRFHFHHS
                     HPHYHEKPSPQVHIHRHDHHDETNHTHIHSKSTVQNSITALLIGTLHGFAGISHFLLI
                     LPTLALPSVSESVIYLAGFAAGTISAMIIYAFIMGYIGQKTANFAYSAAFRNLRIIAG
                     ALAIAVGVWWLF"
     gene            complement(911..1525)
                     /locus_tag="PKZ74_12530"
     CDS             complement(911..1525)
                     /locus_tag="PKZ74_12530"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HPM93626.1"
                     /translation="MMTTKGQDIHGHSGQSGVIFSGFPVHQEDYARDVKEIMAETIKK
                     YGEEEWRLVALTSEIHGHLGIYAIIGAKMGLYAKEILEAGHDELRITSYAGSKPPVSC
                     LNDGLQVSTGATLGHGLIKIGESESPLPAATFTLHDKSIRLTLKRDIFAGIENQIKAA
                     LKESGGLTPAYWVRIRELGLAVWKGYDRKGLFEVIEGTGTLKIN"
     gene            complement(1605..1997)
                     /gene="nikR"
                     /locus_tag="PKZ74_12535"
     CDS             complement(1605..1997)
                     /gene="nikR"
                     /locus_tag="PKZ74_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPM93627.1"
                     /translation="MNIKRFGVSLEDELLQALDEYARQNKFPNRSQAIRHLIRKNLIK
                     RQWEGNQEVAGAIVLVYDHHKRDLQHNSTHIQHDYHDLILSVQHVHLDHHHCLETIAV
                     KGRADRLTDLANKLIGLKGIFHGELVMS"
     gene            2099..2854
                     /gene="truA"
                     /locus_tag="PKZ74_12540"
     CDS             2099..2854
                     /gene="truA"
                     /locus_tag="PKZ74_12540"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPM93628.1"
                     /translation="MHHRYFIKLAYNGARYHGWQIQDNAITVQQVITDAVKLMWSRDF
                     RMIGCGRTDTGVSAKQFYAHFDLPDEKSREELSGLAHRLNRYLPEDIVIYDIFPVPPD
                     LHSRYDAVFRTYEYHIHTRKDPFLNPMSWYVYPAPGVDRMNEACLVLKEYEDFTSFSK
                     FNKKRKTNLCTILDAGWLKDDHRLIFHITANRFLHDMVRAIVGTMMDVGHQKITLEEL
                     RQIIESKDRCRAGESVPAKGLYLTRVEYPVAIG"
     gene            complement(2820..3782)
                     /locus_tag="PKZ74_12545"
     CDS             complement(2820..3782)
                     /locus_tag="PKZ74_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPM93629.1"
                     /translation="MAIYLNRGNVATKTGFGEGVREAARINPNVVGLGADITASVGMN
                     LFADEFPERFYSLGIAEQNCVGVAAGMALAGKVPVFSTYGVFAALRTTDFIRISLCYN
                     NLHAIIGGAHAGISVGPDGATHQALEDLSVMRSLPNMTVISPCDATQTRILTIKAINE
                     QNGPVYVRFGREPVPDFSNGNGKIEIGKSELLIDGEDATIIATGHLVWAAIQAAAILK
                     ESGINVRVLNMHTIKPIDKEAIIEAAVETGRIVTAEEHQVTGGLGSAVAEVIAQRYPV
                     PVRFVGMPDCFGESGKPDELMKKYGMSVEAVCEAIRSLPGIPRG"
     gene            3901..>4242
                     /locus_tag="PKZ74_12550"
     CDS             3901..>4242
                     /locus_tag="PKZ74_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM93630.1"
                     /translation="MVYLDYNATTPVHPAVAEVIRPYFEKFFGNPSSSHAYGIEARLA
                     VEKARNQVSKLIGCKSSEIIFTSGGSESNNMAVKGAALANRAKGNHIITSSIETASAC
                     CCTVSMIPATIE"
BASE COUNT         1120 a         1077 c          935 g         1110 t
ORIGIN      
        1 agcggaattt ttacggcatc atcaatgaat atcgtataaa agaagcgaaa gatcttataa
       61 tggatcaaga gaacagccag atcaaaattg aggttcttgg cgagatggtg ggtttccagt
      121 caaaatcttc ttttaatgcc tgctttaaaa aaatgaccgg tatgacaccc tccgaataca
      181 ggcacaagca taaaaacaat ttctcaaaat aaccaccata cacccactgc gattgccagg
      241 gcgccagcga ttatcctcag gttccggaat gcggcagaat aggcgaaatt cgctgttttt
      301 tgcccgatat atcccatgat aaatgcgtat atgatcatcg cagagattgt ccccgctgcg
      361 aatcccgcca ggtagataac cgactcgctg acagaaggca atgccagtgt aggcagaatc
      421 agcagaaaat gagaaattcc ggcaaatcca tgcagcgttc cgatcaataa tgcagttatg
      481 ctattttgaa cagtagattt tgagtgaatg tgggtatgat tggtttcgtc atgatgatcg
      541 tgacgatgga tatggacctg tggagatggt ttttcgtggt aatgaggatg ggaatgatgg
      601 aaatgaaacc gtcccagcgt tttgacaatg gcccaggcgc caattccgat caatacaaat
      661 ccgaccagaa actcactgta acccgaaatc ttttccacgt tgatcataga tttgaatgcc
      721 aggtagagaa gcccgataag caacatcccg gcaacatggc cgatgcccca catcagaccg
      781 atattccatg cttttttgcg gttctctatg gccagcgggg tcaccgctgc caggtggtcg
      841 ggccctgtca caacatgcac ggccgctgcc agaaaacccg taagtaattg aatcataact
      901 taaaaaatgt ctaattaatt tttaacgtac cagtaccttc tattacttca aacaaccctt
      961 tcctgtcata acccttccag actgcaagcc ccaactctcg tatccggacc cagtatgccg
     1021 gcgttagacc tcccgattct tttaaagcag ccttgatctg gttttcaatc ccggcaaaaa
     1081 tgtctctttt taatgtgagc ctgatcgact tgtcgtgaag cgtaaaggtg gcagcaggca
     1141 aaggactttc ggattcgccg attttgatca gtccatggcc cagtgtggct cccgtgctga
     1201 cctgcagtcc atcgttcaga caactgaccg gcggttttga tccggcataa gaggttattc
     1261 gtaactcatc atgccccgct tccaggattt cttttgcata caaacccatc ttggcgccta
     1321 tgattgcata aatccccagg tgaccgtgaa tttcactggt aagtgccacc agccgccact
     1381 cctcttctcc atattttttt attgtttcag ccatgatttc ctttacatcc ctggcataat
     1441 cctcctgatg aacaggaaat ccgctgaaaa ttaccccact ttgcccggaa tgcccgtgta
     1501 tgtcctgccc ttttgttgtc atcattatca ttatcaataa tatcgtaaat atatctttca
     1561 taacctaatt ccatcgaaca tcgacaatcg acaatcgaca atctctaact catcaccaac
     1621 tctccatgaa agatcccttt caggccgatg agtttgttgg caaggtcggt cagcctgtca
     1681 gccctccctt tcaccgcaat ggtctccagg caatgatgat gatcaagatg aacatgttgt
     1741 accgaaagga tcaggtcgtg atagtcatgc tgaatgtgag tcgaattgtg ctgaaggtcc
     1801 cttttatgat gatcataaac cagcacaatg gctcccgcaa cttcctggtt accttcccat
     1861 tgccttttga tgagattttt ccggatgaga tggcggatgg cctgcgaccg gtttgggaat
     1921 ttgttctgcc gggcatattc atccaacgcc tgaagcaatt catcttccag cgatacacca
     1981 aatcttttta tattcatggt aacacgaatt ttaaaatgat aacacaaaag taacaaattc
     2041 gatgaagcat agcgattgat cagcggattc ctttaaattt gaaacgattt taaataagat
     2101 gcatcaccgg tactttataa agctggcgta taacggcgcc cgttatcatg gatggcagat
     2161 ccaggacaat gccattaccg tgcagcaggt tattaccgat gcggttaaat taatgtggtc
     2221 cagggatttt cggatgatag ggtgtgggcg gaccgatacc ggtgttagtg caaagcagtt
     2281 ctatgcccat ttcgatctgc cggatgaaaa gagcagagaa gagctatccg gcctggctca
     2341 ccgcctgaac cggtatcttc cggaagatat cgttatctac gatatctttc ccgttccacc
     2401 tgacctgcat tcacggtatg atgccgtctt caggacttac gaatatcata ttcacacacg
     2461 gaaggatcca tttctgaatc cgatgtcgtg gtatgtttat cctgctccag gtgtcgatcg
     2521 catgaatgaa gcttgtctgg ttttaaaaga atatgaggac tttacgagtt tttcaaaatt
     2581 taataagaaa aggaaaacga atttatgtac gattctggat gccggctggc tgaaggatga
     2641 ccacaggtta atcttccata tcacggctaa ccgcttttta catgatatgg tccgcgcgat
     2701 tgtcggtacg atgatggatg tgggccatca aaagatcacc ctggaagaac tcaggcaaat
     2761 tattgagagc aaagaccggt gcagggcagg ggagtcggtc ccggccaaag gcttgtacct
     2821 tacccgcgtg gaatacccgg tagcgatcgg atagcttcac agacagcttc aaccgacatc
     2881 ccatatttct tcatcagttc atcgggctta cctgattccc cgaaacaatc cggcattcca
     2941 acaaatctga ccggcaccgg gtagcgttgg gcgatcacct cagctacggc acttcccagc
     3001 ccaccggtca cctggtgttc ctcggcggtc acgattcggc cggtttccac cgctgcctcg
     3061 ataatcgctt ccttatcaat gggtttgatg gtgtgcatat ttaaaaccct cacgttgata
     3121 cccgattcct tcaaaattgc ggcagcttgt atcgccgccc agacaaggtg cccggttgca
     3181 atgatcgtcg catcctcccc gtcaatcaat aattccgatt ttccaatttc gattttccca
     3241 tttccatttg aaaaatcagg taccggttct cttccaaacc ttacgtatac cggcccgttc
     3301 tgttcgttga tggcttttat cgtcaggatg cgcgtttgcg ttgcatcaca gggcgaaatg
     3361 accgtcatat tcggcaacga acgcatgacg gacagatcct caagggcctg gtgtgtggcg
     3421 ccgtcaggtc caacagaaat tccggcatga gcccctccga taatagcatg tagattgttg
     3481 tagcataatg agatacggat gaaatcggtg gtccgaagcg cggcaaaaac accataggtc
     3541 gaaaataccg gtaccttgcc ggccagagcc atcccggcag ccacaccgac acaattctgc
     3601 tccgcaatgc caagggaata gaaccgttcc gggaattcgt ccgcaaacag gttcatccca
     3661 accgatgctg tgatgtcggc ccctagcccg accacatttg gattgatccg cgctgcctcc
     3721 cgcactccct caccaaaacc ggttttggtt gcaacattgc ctcggtttaa atatattgcc
     3781 atccttataa tatatgaatc ccgacgaact atcggatcgt aaaattagca aatcctgaaa
     3841 tcatattaac tttgaacttt tcacttttca ctttgaacct tgaactttaa actaaaccaa
     3901 atggtatatc tcgattacaa cgctaccacc cctgtccatc cagccgttgc ggaggtgata
     3961 cgtccttact tcgaaaaatt tttcggcaat ccttccagca gccatgctta tggtatcgaa
     4021 gcccggctgg ccgtagaaaa ggcccgaaac caggtgtcga aactgattgg gtgcaaaagc
     4081 tccgaaatta tctttaccag cggtggcagc gagtcgaata atatggcggt caaaggtgcg
     4141 gcattggcaa acagggccaa aggcaaccac attatcacca gcagcatcga gaccgcctcc
     4201 gcctgctgct gcaccgtatc catgatcccg gccacgattg ag
//
LOCUS       DAOPNH010000282         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24712_length_4224_cov_6.202686, whole genome shotgun sequence.
ACCESSION   DAOPNH010000282 DAOPNH010000000
VERSION     DAOPNH010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24712_length_4224_cov_6.202686"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKZ74_12555"
     CDS             complement(<1..301)
                     /locus_tag="PKZ74_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93631.1"
                     /translation="MKKLVLSLAIIGFVAFGAIGIQNVVASTAQVEVVNFDKDPKKDE
                     NKKAADNKDVKAETKTTEGASKSCASSCASTCTDKSETAKSCCAGEKSSCEKSCPD"
     gene            445..1689
                     /locus_tag="PKZ74_12560"
     CDS             445..1689
                     /locus_tag="PKZ74_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM93632.1"
                     /translation="MKKNSFLLVVLINSAALIGIIITQYFWIRNAFNLLEEDFAGSVR
                     IAVKSVASQIPAGDVQLISANISQVEYRIGEEFKILRLDEEFHFAILDNRNNSILGGK
                     YGHYDRQILTSAHKIPIPGTGDEYSLAVFFPQENNHIISKLTGWIAIWIIFTLVLIIG
                     FPVSIFIFNRQKKLSGMKTDFINNMTHEFKTPIATISLAGEMLLNEKVLEDKRKIEKY
                     ARVILDENTRLQNHVEQILSVSQLERGQFRLKKKELDVHQLIAEVVKTFSLSFNESGG
                     TIKTHYCANRFIIKADKEHLTNVIFNLLDNAKKYSKENPWIRIGTQNVSNGLVISVED
                     KGIGISPENQRHIFRNLYRVPTGNLHKVKGFGIGLFYVKTIVEAHGGHINLTSELNKG
                     SRFDLFLPFVYNLNKEDEYTQT"
     gene            1670..2377
                     /locus_tag="PKZ74_12565"
     CDS             1670..2377
                     /locus_tag="PKZ74_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM93633.1"
                     /translation="MNTPKPRILLVEDDPNLSLVLQDYLEMMNFDIHLCRNGKEGLDA
                     FRNRKFNICILDIMMPVMDGFTLAEEIRKTDDTVPIVFLTAKSLKEDRIKGFRMGCDD
                     YITKPFSTEELSLRIQAILKRCMNQPARAEKEEPPVYNLGNYIFDHKNLILSINGEQK
                     VLTRKENALLKLLFERQNQLLPREVALKSVWGNDDYFIGRSMDVFITKLRKYLKDDPS
                     IFITNVHGTGFKLEVKK"
     gene            complement(2378..>4224)
                     /locus_tag="PKZ74_12570"
     CDS             complement(2378..>4224)
                     /locus_tag="PKZ74_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM93634.1"
                     /translation="TGRIKQTDMSVPYVENGYYYYTRYEEGKEYPLYCRKKGSLDSVE
                     EIMLNVNEMAAGYAYYDVGGLEVSDDNRLLAFSVDTVSRRIYTLYFKNLETGEIYPDR
                     IGQTSGNLAWAADNKTLFYSTRDESLRPDKIWKYRLGQSEKESELVYFEEDVTYNTFV
                     YRSKSRKYLMIGSGSTMSDEYRYMDAGDPNGRFTVFQPRIRGLEYSVAHYEDRFYIRT
                     NDNAVNFRLMETPEGATGRENWKEVIGHREDVLLEDFDVFKGFLAIQERKEGLKTIRI
                     FNRAGLDYYLDFTEQAYTVTLGSNAEMETEVVRYNYTSLTTPMTVYDYNTRTHESEMK
                     KRQEVVGGYEPGDYFTERVWVTASDGKQIPLSMVYRKDLKRDGHVPVLLYGYGSYGYS
                     TDPYFNSVRLSLLDRGFIYAIAHIRGGEEMGRMWYEDGKLMNKKNTFTDFIACGEYLV
                     NRGYTNPGQLYAMGGSAGGLLVGAVINMRPDLFKGVIAAVPFVDVVTTMLDESIPLTT
                     SEYDEWGNPTDSAYYKYMLSYSPYDNVAAMSYPALLVTTGLHDSQVQYWEPAKWVAKL
                     RVTKTDKNPLYLWTNMDYGHGGASGRFERYRETALEYAFLLDLEGVSE"
BASE COUNT         1164 a         1044 c          899 g         1117 t
ORIGIN      
        1 tatcgggaca agatttttca caggatgact tttcgccggc acagcatgac tttgcagttt
       61 ctgatttgtc ggtgcaggtt gaagcacacg acgaagcgca ggatttcgaa gcgccttcgg
      121 ttgtcttggt ttcagccttt acatctttat tgtcggcagc ttttttgttt tcatcctttt
      181 ttggatcctt atcgaaattg acgacttcaa cctgagccgt tgaagcaaca acattctgga
      241 tgccaatggc gccaaaagct acaaatccta tgattgcgag tgataaaacg agttttttca
      301 taattttacc tgtgttatat taataattat taatgcacaa atttaccccc atataacgga
      361 caggcttgtt aaccggttgt taattactgt taatgatttg ttaaatgggg tcacgaagcc
      421 gctatacggc ctaatttagg agccatgaag aaaaactcct tcctgctcgt tgtgctgatt
      481 aattcggcag ctcttatcgg gattatcatt acacaatatt tctggataag gaatgccttc
      541 aatctcctgg aagaagattt tgccggatcg gtgagaatcg cggtcaaaag cgtcgcgagt
      601 cagattccgg ccggcgatgt tcaattgatt tctgccaaca tcagccaggt tgaatacagg
      661 atcggtgaag aatttaaaat cctccgcctg gatgaagaat tccattttgc aattttagat
      721 aacagaaata attccatcct ggggggcaaa tatggtcatt acgaccggca aatactaaca
      781 tcagcccata aaatcccgat cccgggcacc ggggatgagt attccctggc ggtatttttc
      841 cctcaggaaa acaaccacat tattagcaag cttaccggat ggatcgctat ctggatcatc
      901 ttcaccctgg tgctgatcat cggttttccc gtttccatct tcatttttaa ccggcagaaa
      961 aaactgtcgg ggatgaaaac ggacttcatt aataacatga cgcacgagtt caaaacccca
     1021 atagccacta tctcccttgc cggtgaaatg ttactgaacg aaaaagtgct ggaagacaag
     1081 cgtaagattg aaaaatatgc ccgggtgata ctcgacgaaa atacccgttt gcaaaaccat
     1141 gtggaacaga ttttaagcgt ttctcaactg gaacgaggac aattcaggct taaaaagaag
     1201 gaattggacg ttcatcaatt aatcgcagaa gttgtcaaaa ctttttctct atctttcaat
     1261 gaatcagggg gtaccattaa aacccattat tgcgctaacc gtttcatcat caaagcagat
     1321 aaagaacatc ttaccaatgt tatcttcaat ttgctggata atgcaaaaaa atactcgaag
     1381 gaaaatccct ggatcagaat cgggacgcaa aatgtctcaa acggcctggt gatctcggtg
     1441 gaagacaagg gaatcgggat cagcccggag aaccagcgcc atattttcag gaacctctac
     1501 cgggtgccga cgggtaacct ccataaagtt aaaggatttg ggatcggttt attttatgtc
     1561 aaaacgattg tggaagcaca cggcggacat attaatttaa caagcgaact gaataaggga
     1621 agcagatttg atttattttt accttttgtt tataacttaa acaaggaaga tgaatacacc
     1681 caaacctaga attctcctgg ttgaagatga tccgaatctg agcctggtgc ttcaggatta
     1741 tctggaaatg atgaatttcg acatccattt gtgccgcaat ggcaaggaag gccttgatgc
     1801 tttccggaat aggaaattca atatttgtat tctcgatatt atgatgccgg tgatggatgg
     1861 atttaccctt gctgaagaaa tcagaaagac tgacgatacg gttccgattg ttttccttac
     1921 cgccaaatcg ctgaaagaag accgtatcaa gggattcaga atgggttgcg acgactatat
     1981 tacaaaacca ttcagcaccg aagaactgag tctccgtatc caggctattc ttaaaagatg
     2041 catgaatcag ccggcccggg ccgaaaaaga agaaccaccc gtatacaatc tggggaatta
     2101 catcttcgac cacaaaaatc tgatcctgag cattaacggt gagcaaaagg tgctgacgcg
     2161 taaggagaat gcccttctga agttactttt tgaacgtcag aaccagttgc tcccgagaga
     2221 agttgcacta aaatcggtct ggggaaacga tgattacttt attggcagaa gtatggatgt
     2281 ctttattact aagctcagaa aatatttaaa agacgaccca tccatcttta tcaccaatgt
     2341 tcacggaaca ggcttcaaac tggaagtaaa aaaatagcta ctccgagacg ccttccaggt
     2401 cgagcaaaaa agcatactcc agcgcggttt cccggtatct ttcgaaccgc ccggaagccc
     2461 cgccgtggcc gtaatccata ttggtccata aataaagcgg gttcttatcg gtcttggtta
     2521 ccctgagttt agccacccac tttgccggtt cccagtactg aacctgcgag tcgtgcaacc
     2581 ctgtcgtgac aagcaatgca ggatagctca tcgccgcaac attatcatac ggcgaatagg
     2641 agagcatata tttataataa gcagaatcag tgggattccc ccactcatcg tactcactgg
     2701 tagtcaaggg gatgctctca tccagcatgg tcgtgaccac atcaacaaat ggaactgccg
     2761 cgattacacc cttgaataga tcgggccgca tgttgatcac cgcgccgacc agcaggccgc
     2821 cggcactccc gcccatagca taaagctgcc ctggattcgt atatccacga ttgaccaggt
     2881 attcgccgca ggctataaaa tcggtgaacg tattcttctt gttcatcaat ttgccatctt
     2941 cataccacat ccggcccatc tcctccccgc cccggatatg ggcaatagcg tagataaaac
     3001 ccctgtcaag caaactgagc ctcacggaat taaaataagg atcggtcgaa taaccgtatg
     3061 aaccgtaacc gtatagcagg accggaacat gcccgtcccg cttcaaatct ttccggtaaa
     3121 ccatcgacag gggaatctgt tttccatcgg atgcagtgac ccacacgcgt tcggtaaaat
     3181 aatccccggg ttcgtaaccg ccaaccacct cctgcctctt tttcatttcg ctctcatggg
     3241 tcctcgtatt ataatcataa accgtcatgg gagttgtcaa ggaggtgtaa ttgtacctga
     3301 ccacctccgt ctccatctcc gcattgctgc ccagggtgac cgtataagcc tgctctgtga
     3361 aatccaggta ataatccagg ccggcgcggt tgaaaatacg gatggtcttc aatccctctt
     3421 tgcgctcctg aatcgccaga aatcctttga acacatcaaa atcctccagc aagacatcct
     3481 cccggtgccc gatcacctct ttccagttct ccctgccggt cgccccttcc ggagtctcca
     3541 tcagcctgaa gtttaccgca ttatcattgg tccggatgta aaacctgtct tcataatgcg
     3601 caaccgaata ctcaagcccc ctgatcctcg gctgaaacac cgtaaaccgt ccattcggat
     3661 caccggcatc catgtaacgg tattcatccg acatcgtgct ccctgatccg atcatcaggt
     3721 atttcctgga tttactcctg tagacaaagg tgttatacgt aacatcttct tcaaaataaa
     3781 ccagttcgct ttctttttct gattgtccga gcctgtattt ccagatctta tcgggccgca
     3841 gtgactcatc ccgcgtcgaa taaaacaagg tcttgttatc ggcggcccag gccaggttgc
     3901 ctgatgtctg gccaatccgg tcaggataga tctcaccggt ctccaggttt ttgaagtaca
     3961 gggtgtagat ccggcggctc acagtatcaa ccgaaaaagc aagcaggcgg ttgtcgtcgc
     4021 tgacttccag tccgcccaca tcataataag cgtacccggc agccatctca ttgacattca
     4081 gcatgatctc ctcaacactg tccaggcttc ctttttttct gcaatataaa ggatattctt
     4141 tcccctcttc gtaccgggta tagtaataat aaccgttttc cacgtaaggc accgacatgt
     4201 ccgtctgctt gatccttccc gtta
//
LOCUS       DAOPNH010000283         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24903_length_4204_cov_7.891299, whole genome shotgun sequence.
ACCESSION   DAOPNH010000283 DAOPNH010000000
VERSION     DAOPNH010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24903_length_4204_cov_7.891299"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..578
                     /locus_tag="PKZ74_12575"
     CDS             159..578
                     /locus_tag="PKZ74_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93635.1"
                     /translation="MKKTVFSLAIIMALVFTGMQSAIAQISVEVTWTLCDDTCFTWDD
                     CRYRADVIVYNQCGEEQFEMCKSFETADCWETSATVDLDCSCLDTSTEPCYLVVVTVH
                     KLCIGHGGTITVCSGYGYDHFTCGELMNGVSVESTWQ"
     gene            1487..4099
                     /locus_tag="PKZ74_12580"
     CDS             1487..4099
                     /locus_tag="PKZ74_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM93636.1"
                     /translation="MKLISDSALFKDSGILVGTGPIWGLQLARDGKIYCTPHYTAPEA
                     DQYYLSVVHKPWIRGEGCQFEKNNLNFFPHDVGWTLPNILVDYLLRFEWTGETCQGYP
                     IQFKPNFIPMPQTIQWDFDDGAGSTSWQLSPTYSFKNPGIHEVKVDVWYPSGRYEHTS
                     REIEISPSPLPLLGPDTLICNGASITLNANCDADFFSWSTGQFGVSSITVSDSGTCWV
                     RGRFNETGCEGYDTIHIGFHAPTIIDDTNLQIIPTTCNGASGSITGLTALGPTPYAFQ
                     WLDLSGNDFGTSIDATNLPAGQYQLTIADGNGCETVSEVYTIEDAGNLQVLNVELAQP
                     HCGRPDGEIVIHAFSPAGSALQYSIDDGVTYQADSVFSGLVAAGYVVRVTDGAGCFGF
                     YIDNPVLLEDIPGPQVQPPVVIDETDFLGNGSIEITSTGSTTTLYYSNDNGITYQSNN
                     GTFNNLAAGTYICIVKDENDCDTTFTVEIQNIILTYLHAVTSEGGHCLGNTAMVPVNV
                     DNFNSVATFHLKLSYNADNLQCEGFTNVHPQLSDSLTGWVDQASGTLNLAWNSPSPLT
                     FNQPETVAELVFTTKNPGQGELTWYTGETESYFTNSSGNPIPAEFQTGEVNIYEPPEI
                     ILGQSKTVCTGQFVSLMSIAVGNQPPIDYQWIYPDGDTSTNDPLFFSVSSTDAGIYTL
                     LATDRVGCTDQKAIELMVSDNPVAAFHGTDTLEMHTGDVLDAGTGLSSYHWNTGDTTE
                     SIVINTEGMYKVEMESPVGCLGSDSVYVKLVSEEIPEFEIYIPNAFSPNGDGVNDVFR
                     VEADGSRIEDLGLRIYDRWGGEVFSAKGIENGWDGKKAGKYCLGGVYVYKIVFSVDGV
                     PGNKERVGTVMLVR"
BASE COUNT         1172 a          872 c          960 g         1200 t
ORIGIN      
        1 attccgatcg tgcgtactgc tttttctcat gtgacaatat tttcattaga gatatccgat
       61 cggaatactg aacgttcaat ttatctctca tcttaaactg ccaaagtttt aaccgccgaa
      121 aaatcaaact agttattaac cttttaaatc ttacaaatat gaaaaaaaca gttttttcct
      181 tagccattat tatggcattg gtttttaccg gaatgcagag tgcaatcgca caaatcagcg
      241 tagaagtaac ctggactctg tgtgatgata cttgttttac ctgggatgat tgccgataca
      301 gagctgatgt gattgtttat aatcaatgcg gtgaagaaca atttgaaatg tgcaaatctt
      361 ttgaaaccgc agattgttgg gaaacttcag caactgtaga tttagattgc tcttgtttgg
      421 atacatcaac agaaccatgt tatcttgtag ttgttacagt tcataaattg tgcattggcc
      481 atggtggtac tattacagta tgttcaggtt atggttatga tcattttacg tgtggagagt
      541 taatgaatgg tgtgagtgtt gagtctactt ggcaataata tttaatcagt tccggtgtga
      601 ttttacaccg gaacttttta tatctgaaaa ctatatgaaa accgttttga taataactat
      661 aacctttatt gccatccaat cagtctttgc ccaaaatgaa gccaacgtct ggtattttca
      721 gcataaatgt ggtcttgatt tcaattcggg aataccggag attttaaacg atggatttat
      781 taatactgga tgggcaaacg cttcaatttc tgattcattg gggaattatt tatttagtgt
      841 ggattgggat catatttata ataaggatgg aattatgatg aatgggacag gatttgagaa
      901 tgcagatata tcaggtaaag caattgtcaa atggcctaat caaaatggaa tgtatctact
      961 tttcaccaca aaatccggtg gggggtttaa ttattctata attgatatga atctttatgg
     1021 tggattgggg gctgtaattg agaagaatat tccggttgaa tctgcctttg atgcttccgg
     1081 tcgggtagct gttgtgaaaa aagaaagttc agaaaacata tggataatta caagaaaata
     1141 tattgaagat gcatttgcaa ctttccttat cgatgaaaat ggcttcaact ccaatccatt
     1201 gatttcagtt atgccggacc gggatgaaaa tgctagtatt ctttatggct atattaaaat
     1261 ttcttccgat aaaaaaatta taatatcaag ttataatatg tatggtgaaa ttgaagtttg
     1321 cagttttgat tcttcaaatg gaaggataga atatttgtac acaataagga ccccggtaat
     1381 cgggccatat ccgatcaaag ggatcgagtt ttcacccgat gccaaatatt tgtacgcttg
     1441 ttatgacatc ctgaataata acactagcgt aatttatcaa tatgatatga aactcatatc
     1501 agatagtgct ttgtttaagg attcgggaat attagtcggc acaggaccta tatggggcct
     1561 tcaacttgca agggatggga agatatattg cacaccacat tatacagcac cggaagcgga
     1621 tcaatattat ttgagcgtgg ttcataagcc atggattagg ggagaaggat gccagtttga
     1681 aaaaaataac ttaaattttt ttccacatga tgtaggctgg actttaccga atattttagt
     1741 cgactacctc ctccgcttcg aatggaccgg cgaaacatgc cagggatacc cgatccaatt
     1801 taagccgaac ttcatcccaa tgcctcaaac cattcaatgg gatttcgatg atggggcagg
     1861 aagcacctcc tggcaacttt ctccaacata ctctttcaaa aatcccggta ttcatgaggt
     1921 gaaggttgat gtctggtacc cttccgggag atatgagcac acatcaagag agatagaaat
     1981 ttccccttcc cctcttcccc tcctcggccc ggatacacta atttgtaatg gcgcttccat
     2041 cacgcttaat gccaactgtg acgccgattt cttctcctgg tccaccggcc agttcggcgt
     2101 gtcttccatt acagtttccg atagtggaac gtgctgggtg agaggccgct tcaacgaaac
     2161 cggctgcgaa ggctatgata cgatccacat cggattccac gcacccacga tcatcgacga
     2221 taccaatctg caaataatcc caacaacctg caacggcgca agtggctcga ttacaggctt
     2281 aactgctcta ggccctacgc cctatgcatt tcaatggctc gatctatcag gaaatgactt
     2341 cggcacaagc atagatgcaa cgaacctacc ggccgggcag taccaactta ctatcgccga
     2401 cggcaacggt tgtgaaacgg tttcagaggt ttataccatt gaagatgcgg ggaatttgca
     2461 ggttcttaat gttgaactcg cccagcctca ctgcggaaga ccggatggag agatcgtaat
     2521 tcatgcgttt tctcccgctg gttctgcgtt gcagtattcg atcgatgacg gcgtgacgta
     2581 ccaggccgac agcgttttct ccgggctggt tgctgcgggt tacgtggtac gggttacgga
     2641 tggggctggg tgcttcggat tttacattga taatccggtt cttttagaag atattcccgg
     2701 tccgcaggtt cagccgcctg ttgtaataga cgaaacagat ttcctgggga acggctcgat
     2761 tgaaattact tcaaccggaa gcactaccac cctctattac tccaatgaca acggcatcac
     2821 atatcaatcg aacaatggaa cctttaacaa tttagccgca ggtacatata tctgtattgt
     2881 gaaagacgaa aatgactgcg acacgacttt caccgttgaa attcagaata ttatactgac
     2941 atacttacat gctgttacaa gcgaaggagg gcattgtctg gggaataccg ctatggttcc
     3001 ggtgaatgtc gacaacttca actctgtagc gaccttccat ttaaaattaa gctacaacgc
     3061 cgacaacctt caatgcgaag gatttaccaa tgtccatccg caactttccg acagccttac
     3121 cggttgggta gatcaggctt caggcacatt aaacctggcc tggaattcac cgtcaccatt
     3181 gaccttcaac caacctgaaa ctgtcgcaga actcgtattc actaccaaga atccggggca
     3241 gggagaactt acctggtaca ctggagaaac agaaagctat ttcaccaatt cttccggcaa
     3301 tcccatccct gccgagtttc agaccggtga ggtgaacatc tatgagccgc ccgaaattat
     3361 cctgggtcag agcaaaaccg tttgcaccgg gcagttcgtt agcctgatga gcatcgcggt
     3421 tggcaaccag cccccgatcg attatcaatg gatttatccc gatggcgata cttccactaa
     3481 tgatcccctc ttcttttctg tctcctctac agacgcagga atttacacac ttttggccac
     3541 tgatagagta ggctgcacag atcaaaaagc catcgaactg atggtcagcg acaatcctgt
     3601 cgccgctttc catggcaccg atacgctgga aatgcatacg ggtgatgtac tggatgcagg
     3661 aacaggctta tcttcctatc attggaatac gggcgatacc acagaaagta ttgtcatcaa
     3721 taccgaagga atgtacaaag ttgagatgga gtcgccggtg ggttgtttgg gttccgattc
     3781 ggtttatgta aaactggttt cggaagaaat tccggaattt gagatttata ttccgaatgc
     3841 cttcagtcct aacggggatg gggtgaatga tgtatttcgg gtagaagctg atggatctag
     3901 gattgaggat ctaggattga ggatctatga tcgttgggga ggtgaggtgt tttctgccaa
     3961 aggcattgag aacggatggg atggaaagaa ggccggcaag tactgtctgg gtggagtgta
     4021 tgtttacaag atcgttttct cggttgatgg tgttccgggg aacaaggaac gggtcgggac
     4081 ggtgatgctg gtacggtaaa tcgcctcacg cttcacgcct cacgtctcac caaaacaaat
     4141 ctttaatata aattattggg gctaacgttt ggcgcaactg ccggccggtg tttttggccg
     4201 caca
//
LOCUS       DAOPNH010000284         4201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24923_length_4201_cov_5.822479, whole genome shotgun sequence.
ACCESSION   DAOPNH010000284 DAOPNH010000000
VERSION     DAOPNH010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24923_length_4201_cov_5.822479"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKZ74_12585"
     CDS             <1..473
                     /locus_tag="PKZ74_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPM93637.1"
                     /translation="GGNYAASLAPAARAKEEGCSTVLFLDAKEKKYIDEAGPANFFGI
                     KGNTYVTPESHTILPSITNKSLQVLAKEMGLNVEVRKVAVEELAEFDEVGACGTAAII
                     SPIKQIHDRETGQIYKFCSDGNAGPVSTKLYNKLRAIQYGEEPDTHGWCTIVEL"
     gene            481..1695
                     /locus_tag="PKZ74_12590"
     CDS             481..1695
                     /locus_tag="PKZ74_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM93638.1"
                     /translation="MNIAIIGGGAAGFFAAIAAKENHPGSKVIIFEKSGRLLAKVKVS
                     GGGRCNVTNGCDSISELCQAYPRGGKALKKAFYRFSNKDTIRWFETRGVPLAVQDDLR
                     VFPESGDSQSIIDCLLKEAQRLGIMIQTGMPVREIRAEGEKLAIIFAGDENPARLFDR
                     VIVAAGGSPLRRGLDWLEKMGHKIEDPVPSLFTFNMPGEKIKDLMGVSATKALAKIQG
                     TKFLAEGPLLITHWGMSGPAILKLSSFGARELSRMEYKFKLQVNWACEQNNEIVLAYL
                     QEMASTHPQKMLVNLRPHDLPLRLWQYLLQKADLAENKPWAATGRKGLNKLVNILTND
                     VYEVDGRTTFRDEFVTCGGVSLESVDINTMQSKVVKNLYFAGEVLDIDGITGGFNLQA
                     AWTTGWIAGISC"
     gene            complement(1785..2339)
                     /locus_tag="PKZ74_12595"
     CDS             complement(1785..2339)
                     /locus_tag="PKZ74_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPM93639.1"
                     /translation="MVPIAKNITSDDIVFLGDNLTEGFDLQHYFNLPNLKNRGISGDT
                     TYQVLYRLEEIWNSKPAGLFLMIGINDMFQGEDEPVILQNITLIISELQKHSPGTALF
                     VQSILPVNLSVMFADEFIDLSIFSFNNNLRLVCKELNVRYIDLYADFLDDAGELDRQY
                     TYDGVHLSPEGYDLWAKLIRPFLI"
     gene            complement(2388..2720)
                     /locus_tag="PKZ74_12600"
     CDS             complement(2388..2720)
                     /locus_tag="PKZ74_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93640.1"
                     /translation="MGLNKGKHIVGEMDGVRCTIVESGISKERAAFLKELLEFNGYEV
                     RIQEINADPPDDTKTFTLGVTDIVFNPVIAVYQKTLKRKDGKKVTPAFWNQTGIGEGL
                     PYWLVGRA"
     gene            complement(2733..4154)
                     /locus_tag="PKZ74_12605"
     CDS             complement(2733..4154)
                     /locus_tag="PKZ74_12605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPM93641.1"
                     /translation="MTFEDLLLMRHEIVLTIVAIIILIAELALDDKSRLKVIPLAIGL
                     FALHTVIGFLPLRDGVLFGGMFQTTGTITLMKNVLNIGVLIVFIQSWTWIRHPDNHPK
                     VSEYFLLTISTLIGMDFMISSGDFLMFYLGLELATIPLAALAAFERYKNRSAEAGIKL
                     ILSSALSSAVLLYGLSMIYGTTGSIYFNEISGLISANPLQVLAFIFFFSGMAFKISIV
                     PFHLWAADVYEGAPVNITSYLSVISKGAAVFILMMVLYKVFPAITGMWQDVLWVTAVL
                     TMTIGNLFALRQKNLKRFLAFSSISQAGFIFLGIIGSSPLGMSSVIYFALVYIFSNLG
                     AFGVVAAIANATGKENMDDYNGLYRTNPKLSLVMMLALFSLAGIPPVAGFFGKFFLFT
                     AAAEQGFYYLVLIALLNTIVSLYYYLLIIKAMFLTKSDDAIAYFKSDRPTRLGLILCV
                     AGIVVVGFYSPIYEMIKSLSFGI"
BASE COUNT         1139 a         1001 c         1058 g         1003 t
ORIGIN      
        1 tcggcggaaa ctatgccgcc agcctggcac cggctgccag ggccaaggaa gaaggctgtt
       61 ccaccgtatt gttccttgat gcaaaagaaa agaaatacat cgatgaagcg ggccctgcca
      121 acttcttcgg catcaaaggc aatacctatg tcacccccga atcgcatacc attcttccat
      181 cgatcaccaa taaaagcctc caggtgctgg ctaaagaaat gggattgaac gtggaggtgc
      241 gtaaggtggc tgttgaagaa ctggctgaat ttgatgaggt gggcgcttgc ggaaccgcag
      301 ccattatttc gcccatcaag caaatccatg accgcgaaac aggccagatc tataaatttt
      361 gctcggatgg caatgcgggc cccgtctcta caaaactcta caataaacta cgcgccattc
      421 agtacggcga agaaccggac acacacgggt ggtgtacgat cgttgaactc tgatccggag
      481 ttgaatattg ccatcatcgg cgggggagcg gctggatttt ttgctgcgat cgctgcaaag
      541 gaaaatcatc ccggttcaaa agtcatcatt tttgagaaat ccggccggct gctggcgaaa
      601 gtgaaagtct caggcggtgg ccggtgtaac gttaccaatg gttgcgattc tatctctgaa
      661 ctgtgccagg cctatccgcg gggtggtaag gcgctgaaaa aagcatttta ccgattcagt
      721 aataaagaca cgataaggtg gtttgaaaca cgtggtgttc cattggccgt acaggatgat
      781 ttgcgcgttt ttcccgaatc gggcgattca caaagtatta tcgattgtct cttaaaagaa
      841 gcacaaagac tcggtattat gatccaaacc ggaatgccgg tgagggagat cagagcggag
      901 ggtgagaaac tagcaattat ttttgcggga gacgaaaatc cagccaggct ttttgacagg
      961 gttatcgtgg ctgccggagg ctctcccctg cgcagggggc tggattggct cgaaaagatg
     1021 ggccacaaaa tcgaagatcc cgttccgtcg ttgtttactt tcaatatgcc cggggaaaag
     1081 atcaaagacc tgatgggcgt ttcagccact aaggctctgg caaaaatcca ggggacaaag
     1141 tttcttgctg aaggaccttt gctgatcacg cactggggca tgagcggacc ggctattttg
     1201 aaattgtcat cctttggcgc ccgggaattg agccggatgg agtataaatt caaattgcag
     1261 gtcaattggg cctgtgagca aaacaatgaa atagtccttg cttatctgca ggagatggct
     1321 agtacccacc cccaaaaaat gctggtaaat ctccggccgc atgatctgcc gttacgctta
     1381 tggcaatatt tacttcaaaa agccgacctt gctgagaata aaccctgggc tgcgaccggc
     1441 cgcaaagggc tcaataaact ggtcaatatc ctgaccaatg atgtttatga agtggatggc
     1501 cggactactt tcagggatga gtttgtgacc tgcggcggcg ttagcctgga gagtgtcgat
     1561 ataaacacga tgcaaagcaa agtcgttaaa aatctctatt tcgccggaga agtacttgat
     1621 atcgatggaa ttaccggtgg ttttaatctc caggccgcct ggacgaccgg ttggattgca
     1681 ggaatatcct gctgagttca taccaaagtc attcgtataa tccgtgtcat cagtgttcca
     1741 ttatctgaac acaaatacaa tgaattatac aaatttatac agggttaaat caggaatggt
     1801 ctgatcagct tagcccagag gtcatatcct tccggtgaaa gatgtacccc gtcataggta
     1861 tattgccggt ccaattcccc ggcatcatcc aggaagtcgg cgtacagatc aatatagcgg
     1921 acgttaagtt ccttgcaaac cagccttaaa ttgttattaa aactgaagat tgacagatcg
     1981 atgaactcat cagcaaacat aaccgaaagg tttaccggca gaatgctttg tacaaacagt
     2041 gccgttcccg gtgaatgttt ctgcagttca ctaataatca acgtaatatt ttgaaggatg
     2101 accggttcat cctcaccctg gaacatatcg ttgatgccga tcatgagaaa aagcccggca
     2161 ggtttggagt tccatatctc ctccaggcga tagagcacct ggtaggtcgt gtcgccactg
     2221 ataccccggt tcttcaggtt tggaagattg aaatagtgtt gaaggtcaaa gccttccgtc
     2281 aggttgtcgc ccagaaaaac gatgtcatcg gatgttatgt tctttgctat cgggaccacc
     2341 ttgaacctgg aatttggaac ctggaacctg gaacctggaa cctggaacta tgctctccca
     2401 accagccagt aaggcagccc ttccccgatc ccggtctggt tccagaaagc gggggtaacc
     2461 ttttttccgt ctttccgctt caacgttttc tggtaaaccg caatgaccgg attgaagacg
     2521 atatcggtta ccccgagagt gaatgttttc gtgtcgtctg gcggatcggc gttgatttcc
     2581 tggattctca cttcataacc gttgaactcc agcaactctt tcagaaaagc ggccctctct
     2641 tttgatatcc ccgattcaac tattgtgcag cggactccgt ccatttcacc gacaatgtgt
     2701 tttcctttat tcaatcccat tattctgtca aattaaatcc cgaaactcag gctttttatc
     2761 atttcataga tcgggctgta aaatcccaca acgacgatgc cggccacgca aaggatcaat
     2821 cctaaccggg tgggccggtc gcttttgaaa taagcaatgg cgtcgtcgct tttcgtcagg
     2881 aacatagctt tgatgatcag cagatagtag tagagcgaaa cgatcgtatt cagcagagcg
     2941 atcaggacca ggtagtagaa tccctgttct gccgcggcgg taaacaggaa gaactttccg
     3001 aagaacccgg caaccggagg aatacccgca agggagaaca gcgccagcat cataaccagg
     3061 ctcagtttcg gattggtgcg gtacaaaccg ttgtagtcat ccatattctc ctttcccgtt
     3121 gcatttgcga tagcagccac cacgccgaat gcgcccaggt tggagaagat atagacaagt
     3181 gcaaagtaga tcaccgaact catcccgagc gggctgctgc cgattatgcc caggaagata
     3241 aacccggcct gcgagatgga tgagaaggca aggaaccgct tcagattctt ttgcctcagt
     3301 gcgaataagt taccgatggt catcgtgagt acggcagtga cccataaaac atcctgccac
     3361 atgccggtaa tggccgggaa aactttgtac agcaccatca tcaggataaa aaccgctgcg
     3421 cctttggaga tcaccgaaag gtaagaggtg atattgaccg gagcgccttc gtaaacatcg
     3481 gcagcccaca ggtggaaagg cacgatcgag atcttgaagg ccattccgct gaaaaagaag
     3541 atgaacgcca gtacctgcag cggattggcc gaaataagcc cggagatctc gttgaaatag
     3601 atcgaacccg ttgtcccata gatcatcgac aggccataaa gcaggacagc cgaagacagg
     3661 gcagaggata ggatgagttt gatgccggct tcggcagaac ggtttttata ccgttcgaaa
     3721 gcagccaggg cggccagtgg tatagtagcc agttccaggc ccagatagaa catcaggaaa
     3781 tcgcccgagg agatcatgaa atccataccg atcagggttg aaatggtaag gaggaaatat
     3841 tcgctgacct tcggatggtt atccggatgc ctgatccagg tccacgactg gatgaaaacg
     3901 atcagcacac cgatgttcag cacattcttc atcaatgtga tcgtgccggt tgtctggaac
     3961 attccgccga acaatacgcc atcccgtaag ggtaaaaagc cgatgaccgt atgcagggca
     4021 aacaagccga tggccagcgg tattaccttt aacctggatt tgtcatccag cgccagctcg
     4081 gcgatcagga taatgatggc aacgattgtc agtacaatct catgccgcat cagtaacagg
     4141 tcttcaaatg tcatatcggg ttaaattgtt ctattgttaa attgttatta ttcacttcac
     4201 c
//
LOCUS       DAOPNH010000285         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_24963_length_4197_cov_5.730082, whole genome shotgun sequence.
ACCESSION   DAOPNH010000285 DAOPNH010000000
VERSION     DAOPNH010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24963_length_4197_cov_5.730082"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="pepE"
                     /locus_tag="PKZ74_12610"
     CDS             <1..376
                     /gene="pepE"
                     /locus_tag="PKZ74_12610"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPM93642.1"
                     /translation="VIRDRANDGMHYMGWSAGANVACPTLRTTNDMPIVQPDSFRTCD
                     LVPFQINPHYLDANPEGHGGETRQQRIEEFLAVNRDITVIGLRESSLIQVEGDKYELK
                     GKRPMRVFRYGLEPVETVSFDY"
     gene            complement(391..1089)
                     /locus_tag="PKZ74_12615"
     CDS             complement(391..1089)
                     /locus_tag="PKZ74_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93643.1"
                     /translation="MKKPALILLMVLLTCSGITQVNPTFIGLKTGVSVPVGQFGGHDL
                     VTGSFTQAGFQGAIEGAWFFKPWIGAGLHGFAALHPIDVGTLGYEKVIADPFMDDVTI
                     RSEPFRTFGGYAGLFFHVPLVKNLSVTGKALGGMIYAKTPYQLYKAEYYLVGEQWFEI
                     TSAADYEASFLAGVGIRYDINKGTAFCLDTEFTWNKADFEFITTGNDIRIEEKDISFL
                     NIGVGVIWKISAGE"
     gene            complement(1091..1258)
                     /locus_tag="PKZ74_12620"
     CDS             complement(1091..1258)
                     /locus_tag="PKZ74_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93644.1"
                     /translation="MIIGLFIYSLIFIAVGILFALRKKKLIAAMFFLLALLLAVVGVA
                     AIVVYPHIWPF"
     gene            1299..2111
                     /locus_tag="PKZ74_12625"
     CDS             1299..2111
                     /locus_tag="PKZ74_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM93645.1"
                     /translation="MNDPHSNEMELKKTAVVTGATGAIGKAIVRQLAASGNYNVIMVA
                     RDESKAIRVVKDIQAKTGSLDIVYELADLSEKVSIYELASRINRPVHVLINNAAFTPQ
                     KRIENSEGIEMQFAVNVMGYFRMIDALTPQLKAGAPARIVNVASYWAGGLDLDDPEFR
                     KRKYDNDSAYRQSKQANRMLTVAFARKLMPYRITVNSCHPGDVNSALSNSLGFGGHES
                     PDQGADTPVWLATSRETQEITGKYFEHRKEAPCSFSQDSQKVGLLYEYCSCR"
     gene            complement(2215..2997)
                     /locus_tag="PKZ74_12630"
     CDS             complement(2215..2997)
                     /locus_tag="PKZ74_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPM93646.1"
                     /translation="MNIPFYQVDAFTDRLFGGNPAGVCPLEEWLPDEVMQKIAMENNL
                     SETAFYIRKEEGYHIRWFTPKVEVNLCGHATLASAHIIFQTTGISDDTIPFQSRSGLL
                     NVRKEGDLLVLDFPANKPQRTGLPEDFIHSLNITPIQCHRGKEDYLLLYKTQQEIEAL
                     LPDFKRLEKTDARAVIVTAPGNHVDFVSRFFAPRVGVDEDPVTGSAHTVLIPFWAEKL
                     GKNEMKALQLSRRGGSIFCRLRDDRVDIGGKAVTYLKGEITI"
     gene            complement(3116..>4197)
                     /locus_tag="PKZ74_12635"
     CDS             complement(3116..>4197)
                     /locus_tag="PKZ74_12635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM93647.1"
                     /translation="HVEPGETTIEAAFREAQAIAEEIRSRNELKDVYEGLSGIYSKTG
                     DFKNAYNYQSLYTLMKDTLYNDEMDKKLQSMTLSYEVEKKQGDIDLLTKDNALQELNL
                     QRQRIAIYAAGSTGFLLLLLALGIFNRYKYVRRTKKIIENEKDRSEKLLLNILPVETA
                     QELKEKGFATPKHYDVVSVLFTDFKGFTKIAEKLTAQELIEELNYCFFEFDKIIDKHN
                     LEKIKTIGDAYMCAGGIPVANKSNPVDIVRAGLEIKEFMEKLRSDKEKQGKDYWELRI
                     GIHTGEVVAGVVGKNKFAYDIWGDAVNTASRMESSGIPGQVNISGTTYEYIKEHFVCT
                     HRGKIQAKNKGEIDMYIVERERELI"
BASE COUNT         1105 a         1048 c          936 g         1108 t
ORIGIN      
        1 tgtgatccgt gatcgcgcca atgatggaat gcattacatg ggatggagcg ccggggccaa
       61 tgtggcctgt ccgacgctcc gcacaaccaa cgatatgccg atcgttcaac ccgatagctt
      121 caggacatgc gacctggtac ccttccagat caatccgcat tatctcgacg ccaatccgga
      181 aggacacggc ggtgaaaccc ggcagcagcg gatcgaagag ttcctggcgg taaaccgcga
      241 tataaccgtg atcgggctga gggaatcctc tctcatccag gtggaaggcg acaaatatga
      301 gctgaagggg aaaaggccga tgagggtttt tcggtatgga ctggagcctg tggaaacagt
      361 cagttttgat tactgaaagc tgttactttg ctattctccg gctgaaatct tccagatcac
      421 gccgacgccg atattcagaa aggaaatatc cttttcttca atccggatat cgttaccggt
      481 tgtaataaac tcaaaatcag ctttattcca ggtgaattcc gtgtccaggc agaatgcggt
      541 acctttgttg atatcgtacc ttattcccac accggccagg aaactggctt cataatcggc
      601 tgctgaggtt atctcaaacc attgttctcc aaccaggtaa tattcggctt tgtagagctg
      661 gtaaggcgtt ttggcataga tcatcccccc cagggctttt ccggtaaccg acaggttctt
      721 aaccaacgga acatggaaaa acaagccggc gtatccaccg aaggtccgga aaggttctga
      781 ccggatcgtg acatcatcca tgaacggatc ggctattact ttttcgtaac caagcgttcc
      841 gacatcaatc ggatgcaatg ccgcaaaacc atgtaacccg gcacctatcc agggcttaaa
      901 aaaccaggcc ccctcaatgg cgccttgaaa acccgcctga gtgaaactgc ctgtaacaag
      961 atcatgacct ccaaattgtc ctaccgggac ggacactccg gtttttagac caataaaagt
     1021 gggattgacc tgggtgatcc cgctgcaggt cagcaatacc atcaacagta taagtgctgg
     1081 ttttttcata ttaaaagggc cagatatgag gataaacgac aattgcagcc acaccaacca
     1141 cggcaagcaa cagggccaga aggaaaaaca tggctgcgat cagttttttc ttccgcaagg
     1201 caaagaggat tccaacggcg ataaaaatca gggaatagat aaacaggcct attatcatga
     1261 tattttcaaa acaaatttaa aagcgaaaat aactatattt gaatgatcct cactcgaatg
     1321 agatggaatt aaagaaaaca gccgttgtta ccggtgcaac aggagccatt ggtaaagcaa
     1381 ttgtacggca attagccgca tccggtaatt acaacgtaat aatggtagcc cgcgatgaat
     1441 ccaaagccat ccgggttgtg aaggatatcc aggctaaaac cggttcgctc gatattgttt
     1501 atgaacttgc cgacctgtct gaaaaagtgt caatatacga attggcttct cggataaaca
     1561 ggcctgtgca tgtattgatc aacaatgccg cattcacacc ccaaaagcgg atagaaaatt
     1621 cagaagggat cgaaatgcag tttgcggtca atgtcatggg ctacttccgg atgatcgacg
     1681 cattgacgcc gcagcttaaa gcaggcgctc cggcccggat cgtgaatgtg gccagctact
     1741 gggccggcgg gcttgatttg gatgatcccg aattcagaaa aagaaagtac gataacgatt
     1801 cggcctatcg acaatccaag caagccaacc ggatgcttac ggtcgcattt gcccgcaaac
     1861 tgatgccata ccgcatcaca gttaattcct gccatcccgg ggatgtaaat tcggcattaa
     1921 gtaactcttt aggatttggc ggtcatgaat cgcccgacca gggtgccgat acgcccgttt
     1981 ggctggccac ctcccgtgaa acgcaggaaa ttacgggtaa atattttgag cacagaaagg
     2041 aagccccttg cagtttttca caggacagcc agaaagtcgg gcttttatac gaatattgtt
     2101 cctgcagata atccagtcgc aaaacttaat cctggcaatt tacactttac actttaagat
     2161 ttaagttttg aactttgcac tttgcacttt gcactttgca ctttgcactt tgaactaaat
     2221 tgtaatttcc cctttcagat aagtcaccgc tttcccgccg atatctactc tgtcgtcgcg
     2281 caggcggcag aaaatgcttc ccccgcggcg ggaaagctgc agggccttca tttcattctt
     2341 cccaagcttt tcggcccaga aggggataag aaccgtatgg gcagagccgg taacgggatc
     2401 ttcgtcaaca cccacacggg gagcaaagaa acgggatacg aagtcgacgt ggttcccggg
     2461 agctgttacg attacagccc gcgcatccgt tttctccagc cgcttgaaat ccggtaaaag
     2521 tgcttcaatt tcctgctgcg ttttatacag cagcagataa tcttccttgc cccgatggca
     2581 ttggatagga gtgatattca gggaatggat gaaatcttcg ggaagcccgg tccgttgcgg
     2641 tttattggcc gggaagtcga gcaccagcaa atctccttct ttcctgacat tcaaaaggcc
     2701 gctcctggat tggaaaggaa tagtatcgtc ggagatgccc gtggtctgaa aaataatgtg
     2761 agctgatgct aaagttgcat gaccgcaaag gttcacttcc actttcggcg taaaccaacg
     2821 aatatggtag ccttcttctt tcctgatata aaaagctgtt tcggagaggt tattctccat
     2881 ggcgatcttt tgcatcacct catcgggaag ccactcttca agagggcaaa cccctgccgg
     2941 attgcctccg aataatcggt cggtaaaggc atccacctgg taaaacggaa tattcatgcg
     3001 tattttgatt ttggttcaag gccgcttttg gagttgaaat caaaattaga aaatattcct
     3061 tcccaaaaac taaaatcata aattcataaa atcacattat cacattatca aattatcaaa
     3121 ttaattcccg ctctctctcc acaatataca tatctatctc tcctttgttc ttggcctgga
     3181 ttttcccccg gtgggtgcac acaaaatgtt ctttaatata ttcataggta gttcccgaaa
     3241 tattcacctg tcccggaata ccgctcgatt ccatccggct ggcggtattg accgcatcgc
     3301 cccagatatc gtaggcgaac ttgtttttac cgacgacccc ggccacgact tctccggtgt
     3361 gtatcccgat cctgagttcc cagtaatcct tgccctgctt ctccttgtct gacctgagct
     3421 tttccatgaa ttccttgatt tccagtccgg cccgcacgat atcaaccgga ttcgacttgt
     3481 ttgcaaccgg tatcccgccg gcacacatgt atgcatcccc gatcgtcttg attttctcaa
     3541 gattgtgttt gtctatgatc ttgtcaaatt caaagaagca gtaattgagc tcttcaatca
     3601 gctcctgggc tgtcagtttc tcggcaatct tggtaaatcc tttaaaatcg gtgaaaagaa
     3661 cggaaaccac atcgtagtgt ttcggtgtgg caaagccttt ctctttcagt tcctgtgcgg
     3721 tttcgaccgg caggatattc aacagtagtt tttcggagcg gtctttttca ttctcgatga
     3781 tctttttcgt ccgcctgaca tatttatacc tgttaaaaat acctaaggcc agcaacaaca
     3841 gcagaaatcc cgtactccct gccgcgtata tggcaatcct ctgtcgctgc aaatttaatt
     3901 cctgcagggc attatctttg gtcagcagat cgatatcccc ctgttttttc tccacctcgt
     3961 agctgagcgt catcgactgc agcttcttat ccatttcatc attatacaaa gtatccttca
     4021 tcagggtata taatgactgg taattatatg catttttaaa atcaccggtt ttcgaataaa
     4081 tccctgataa tccttcatat acatccttga gttcgtttct cgaacgaatc tcctcagcaa
     4141 ttgcctgggc ttcacggaat gctgcctcga tggtggtctc tccgggctcg acgtggc
//
LOCUS       DAOPNH010000286         4172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_25194_length_4172_cov_5.778237, whole genome shotgun sequence.
ACCESSION   DAOPNH010000286 DAOPNH010000000
VERSION     DAOPNH010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25194_length_4172_cov_5.778237"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKZ74_12640"
     CDS             complement(<1..419)
                     /locus_tag="PKZ74_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPM93648.1"
                     /translation="MANYSIKDLERISGVKAHTIRIWEKRYGIVEPKRTDSNIRYYCD
                     HDVKKLMNVAILLNHGYKISKLAGLDCSEISKKVLEVSMVANGYDSQIENLVIAMIEL
                     DESRFEKLLNQSIIKDGFENTVFKVLYPFFERIGVLW"
     gene            545..1351
                     /locus_tag="PKZ74_12645"
     CDS             545..1351
                     /locus_tag="PKZ74_12645"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPM93649.1"
                     /translation="MKRLDKEIQDIVVIGSGNVAYHLICAFTGIGISVSQIYSRNEKT
                     AALLSGKFGIPYIVDPNLLNRNAGLYILAVQDSNIREAALSLNLEDHLLVHTSGFSPL
                     GILEGTAAFTGVIWPVQTLTSGKDTDYRSIPIFTEGSCNETRIMLNNLAGRISHKVVL
                     TDTPTRQRVHLAAVISSNLVNHLYSVSASILESVNLPFDILRPLIHETAIKATAALPS
                     DAQTGPAARHDMEVIRQHLEMLRDNPLQRDIYQLITNSILNSGPDKHEKL"
     gene            1338..1859
                     /locus_tag="PKZ74_12650"
     CDS             1338..1859
                     /locus_tag="PKZ74_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPM93650.1"
                     /translation="MKNYKELLRHIKAFVFDYDGTLTDGTVILLDEGEPLRTANVRDG
                     YAIQLAVKKGYQVVIISGGRSKSMSNRFRMLRVQHVFLGIEHKLETLRKFLEENGLEP
                     ENVLYLGDDIPDYHAMKYCGVAACPADAAEEIKSISHYISHYKGGEGCARDVIEQVLK
                     VQGQWMNDDAFAW"
     gene            1862..2809
                     /locus_tag="PKZ74_12655"
     CDS             1862..2809
                     /locus_tag="PKZ74_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPM93651.1"
                     /translation="MNLKAVISLVRPINLAIMAGMLLLVRYAIFEPVFAMNHLSGLMP
                     FWQFLLLILATVLIGAGGYVINDVLDIEIDLINKPDRQIIGKKISVETGNKLHFNLTA
                     SGIVLGIAFSYLSGNIFLAILFVIIPTALFYYSFKYKYLPIAGNLVVALLAALTVIIY
                     WLFEFYHLKKTPEVFIEAASSFAILNRFILAFASFAFLTTLTREIIKDAEDMTGDSRF
                     GCNTLAIVLGQKKVKYLIIALEVVLILLLAWFQLYLHRSGLVLMSWSLLITQVLIVVT
                     LFRTIKTGSNSAFRQLSLFFKIIMVTGMLSLLTVYFRNI"
     gene            2813..3394
                     /locus_tag="PKZ74_12660"
     CDS             2813..3394
                     /locus_tag="PKZ74_12660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPM93652.1"
                     /translation="MFDNLKGRHIILASKSPRRRYLLSQLGLEFSEMAADIDESFPDS
                     LTPDEAALYLAEKKADHFADMISQPANIIITADTLVLLDGNILGKPEGKAGAKAMLKA
                     LSGRMHQVVTGICIRDSKKNRSFTCWTDVYFKELSDEEITYYLEAYQPYDKAGSYGIQ
                     EWIGYIGISGINGSYFNVMGLPVHQLYDELRRF"
     gene            3423..>4172
                     /gene="surE"
                     /locus_tag="PKZ74_12665"
     CDS             3423..>4172
                     /gene="surE"
                     /locus_tag="PKZ74_12665"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPM93653.1"
                     /translation="MNSKPPTILITNDDGIFAPGLRTLIKVMRTMGNVTVVAPDRPMS
                     GSGHAITVRSPLRLHLIAREEGYAEYSCNGTPVDCVKLGEQVVLKAKPDLLVSGINHG
                     SNAAINIIYSGTMAAVLESAIGGVPSVGFSMLDYSHNADFSACEPFVRLIAGRVIEHS
                     LPDGICLNVNIPALNGSEIKGVRICRQGRARWVEEFDTRTDPHKREYHWLTGYFEKLE
                     ENDDTDQWALENHYVSVVPVHFDLTAHYALNT"
BASE COUNT         1064 a         1031 c          988 g         1089 t
ORIGIN      
        1 tgccacagca cgccaattct ttcaaagaaa gggtaaagca ccttgaacac tgtattttca
       61 aatccgtctt taatgataga ctgattcagc agtttctcga acctggattc atccagttcg
      121 atcatggcga tgaccaggtt ctcaatctgg ctgtcgtacc cattggcaac catcgaaact
      181 tccagtacct ttttggaaat ctcgctgcaa tcgagaccgg caagtttgga gatcttatag
      241 ccatgattca gcaggatagc gacattcatc agctttttca catcatggtc acaataatag
      301 cggatattgg agtcggtcct ctttggctcc acgatgccat accgcttttc ccagatacgg
      361 atggtatgtg cttttacgcc gcttattctc tcaagatctt taatgctata gttcgccatg
      421 gaatgatgaa tacgtcggga taaacaatac gaatatgatt ttgttcactt ttaatgcaaa
      481 gcaaattacg cacaatttct gaaatcaaac catccggtca aatatatcgg ttaattttgc
      541 acgaatgaaa aggttggaca aagagattca ggatattgtc gttatcggat cgggaaatgt
      601 tgcctatcat ttaatatgcg cttttaccgg cattggcatt tccgtctccc aaatctattc
      661 ccgaaacgaa aaaactgccg ctcttctgtc cggcaaattt ggtatacctt atatagttga
      721 ccccaacctt ctcaaccgaa atgccggctt gtacatactg gcggtccagg acagcaacat
      781 ccgggaagcg gcgttaagcc tcaacctgga ggatcatctg ttagttcaca catcgggttt
      841 ctccccgctc ggcatcctgg aaggcacagc ggcttttacc ggcgtgatct ggccggttca
      901 gaccctcact tccggaaaag ataccgatta ccgttccatc ccgattttta cagagggttc
      961 ctgcaatgaa accaggatca tgctgaataa cctggccggc aggatcagcc ataaagttgt
     1021 tttaaccgac actccgacgc ggcaaagggt tcacctggcc gcggtcattt catccaacct
     1081 ggtcaatcat ctgtattcag tttcagcatc cattctcgaa tcggttaacc ttccgttcga
     1141 tatcctgcgt ccgttgattc atgaaactgc aataaaggcc accgctgcct tgccctccga
     1201 tgcacagaca ggtccggcgg cccggcacga tatggaggtg atccggcaac atctcgaaat
     1261 gctgcgggat aatccccttc agcgggatat atatcaattg ataaccaaca gtattttaaa
     1321 ctcaggccct gataagcatg aaaaactata aagaactgct ccggcatatt aaagcttttg
     1381 tattcgatta tgatggaacc ctgaccgacg gcacggtcat ccttctcgac gaaggggaac
     1441 cgctgcgaac ggccaatgta agggatggat atgccattca gctggcggta aaaaagggtt
     1501 accaggtagt aattatctcc ggcggaagat caaaatcgat gtcaaaccgg ttccggatgt
     1561 taagggtgca gcatgttttt ctgggaatcg agcataaact ggagacactc aggaaatttt
     1621 tggaggagaa cggcctggaa ccggaaaacg tcctctacct gggcgacgat attcccgact
     1681 accatgcgat gaaatattgc ggtgtggcag catgcccggc cgatgcagcc gaagagatca
     1741 aatccatttc gcattacatt tctcattaca aaggcggcga aggttgtgcc cgcgatgtga
     1801 tcgaacaggt tcttaaagtg cagggacagt ggatgaacga cgatgctttt gcctggtaga
     1861 aatgaatctt aaagccgtca tatcccttgt ccggccaata aacctggcca tcatggccgg
     1921 catgctgctg ctggtcagat atgccatatt cgagccggta ttcgccatga accacctctc
     1981 cgggctcatg cctttctggc aattcctcct gcttatcctg gcaacggttc tcatcggggc
     2041 cggcggttat gtgatcaacg atgttctcga cattgagatc gacctgatca acaaacccga
     2101 caggcagatc atcggaaaaa agatcagtgt ggagaccggg aacaagctcc acttcaacct
     2161 gacagcctcc gggatcgttc ttggaattgc attcagctat ttgtccggga atatttttct
     2221 tgccatcctc tttgtcatta ttcccacagc ccttttttat tacagtttca aatacaaata
     2281 cctgccgatt gccggcaacc tggttgttgc tcttctggca gcattaaccg tgataatata
     2341 ctggctgttt gagttctatc atctgaaaaa aacaccggag gttttcattg aagccgcttc
     2401 atcttttgca atcctgaacc gtttcatcct ggcatttgcc tcttttgcct ttctgaccac
     2461 cctgacgcgg gaaatcatca aagacgccga ggatatgacg ggcgacagcc ggtttggctg
     2521 caatacgctg gctattgttt tagggcagaa aaaggtgaag tatctgatca ttgctttgga
     2581 ggtggtgctg atattacttc tggcatggtt tcagctatat cttcacagat cgggattggt
     2641 gttgatgtcc tggtcgcttc tgatcacaca ggtcctgatc gtggttaccc ttttccggac
     2701 cataaagacc ggctcgaatt cggccttcag gcaattgagc cttttcttta agatcatcat
     2761 ggtaaccgga atgctctcct tattaacagt ctatttcaga aatatttagt ttatgttcga
     2821 caatctgaaa ggccgtcata tcatcctggc gtcaaaatct ccccggagaa ggtatttgtt
     2881 aagtcagctc ggcctggaat tctcggaaat ggctgccgat atcgacgaat cattcccgga
     2941 ttcactgaca cctgacgaag cggcccttta cctggcggaa aagaaagcag accattttgc
     3001 ggatatgatc tctcaaccgg caaacattat catcacggcc gataccctgg tgctgctcga
     3061 cggtaatatc ctcggaaaac cggaaggaaa ggcaggtgca aaagcaatgc tgaaggcgct
     3121 gtcgggcagg atgcaccagg tggtaaccgg catttgcata cgcgacagca aaaagaaccg
     3181 ctcttttacc tgctggaccg atgtctattt caaagaactt tcggatgaag agatcaccta
     3241 ttacctggaa gcatatcagc cttatgataa agccggttca tacggtatcc aggaatggat
     3301 tggttacatc ggcatatccg ggatcaacgg atcatatttt aatgtgatgg gattgcccgt
     3361 gcatcaactt tacgacgaac ttcgccggtt ttgatccggt taaataatat tatctttgcc
     3421 gcgtgaactc aaaaccccct accatactta tcactaatga tgacggtata ttcgcaccag
     3481 gtctgcgaac actgatcaaa gtcatgcgga ccatgggaaa tgttacggtc gtcgcgcccg
     3541 acaggccgat gtcaggctcg gggcatgcca taacggtcag aagcccactt cggctgcatc
     3601 tcatcgcccg cgaagagggt tatgccgaat acagttgcaa cggtactccg gtcgattgtg
     3661 ttaagctggg cgaacaagtc gtattgaaag ccaaacccga cttgctcgtt tcggggatca
     3721 atcatggttc aaacgctgcg atcaacatta tttactccgg aaccatggcc gctgtgctgg
     3781 agagcgcgat cggcggggtc ccctcggtcg gtttttcgat gctggattac agccacaatg
     3841 ctgacttttc tgcatgtgaa ccctttgtca ggctcattgc cggcagagtc atcgaacata
     3901 gccttcctga tggtatttgc ctgaatgtca acataccggc gttgaacggc tccgaaataa
     3961 aaggggtgag aatctgccgg cagggacggg caagatgggt agaggagttc gatacccgga
     4021 ccgatcccca caaacgggaa tatcactggc tgacgggtta tttcgagaag ctggaggaaa
     4081 atgatgatac cgaccagtgg gccctggaaa atcattacgt ttcggtggtg ccggtacatt
     4141 tcgacctcac cgctcattat gccctgaata cg
//
LOCUS       DAOPNH010000287         4131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_25529_length_4131_cov_7.222767, whole genome shotgun sequence.
ACCESSION   DAOPNH010000287 DAOPNH010000000
VERSION     DAOPNH010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25529_length_4131_cov_7.222767"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1058
                     /locus_tag="PKZ74_12670"
     CDS             <1..>1058
                     /locus_tag="PKZ74_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93654.1"
                     /translation="SAFRNLLRNKFYAFLNILGLSVGLAAFIFILIYVLDEINYDKHN
                     EKHARIYRIESDFNISNRHDRFAIVPVPMGPAFKLEFPEVEAVTRLNPVGNALFRYGE
                     KEYYEDDFYFADSNIAEVFTLNYLSGVPEKALNEPFTMVLTKKIAKKYFGDANPMGEI
                     ITTGSGRSYKVTGVIEDLPANSHLRYGALLSTVSLAELYGRDNFNSMEPLAFWNIGVF
                     TFILLNENASMENVVEKFPSFYEKYMKPIGDQISASFSLLYTPLAKTHFAQGLGSELP
                     TGNMAYIYIFSAVAFFILLLATINYMNMATARSANRAREVGMRKVVGAYRNQIISQFL
                     SESVIMALMALVIALCIV"
     assembly_gap    1059..1158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1159..1705
                     /locus_tag="PKZ74_12675"
     CDS             <1159..1705
                     /locus_tag="PKZ74_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93655.1"
                     /translation="QPRYFMTARIKEGREKEALAYIGDKWNTFQAGRPFDYKYVSQIL
                     EEQYGGEKKTGIIFNIATIITIFIALLGLLGLSSFVTEQRTKEIGIRKILGATVPGIL
                     NLLYREFVILILIAFVLAVPVAWWRLDIWLNDSFIYHTSLNWTYFLLAGGLAFAVGIM
                     TISFYIIRAANSNPVDAVKWE"
     gene            1718..4126
                     /locus_tag="PKZ74_12680"
     CDS             1718..4126
                     /locus_tag="PKZ74_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM93656.1"
                     /translation="MYEHYLKTSLRNFWRYRGYTLINMLGLATGIATCLIIFTYISHE
                     LSYDKFHEDYEDVYRVAVKGRFAEDFFNVACSMPPIGPTLKEKFPEVMAFTRIDKFQN
                     NPFLAYEDKKFYEDGLYFADSAFLDVFTFEMINGDPATALVEPNCIVLTEGIADKYFE
                     NENPVGKVLRLNDQVNMKVTGVLRDIPENTHLKFSMIGSISTVNGRLGREPMDENWGS
                     LFLHNYIRLYPGTGAADFGEKIRYVIKDAFGEAAETYNIEMIPYLQPLASIHLYSNLM
                     AELEPGSDISYIYIFAAVALFILIIACINFMNLTTARSTKRSREVGMRKVAGATRKQL
                     IGQFLSESMLISLLCMVFAFITAEAVLPFFSDLTGLNLEPWFHTPVIIPVILGLGLFV
                     GVFAGAYPAFILSSFQPIRAIRGELYRGMKKSVLRNLLVVLQFAISIAMLVSTWLIYK
                     QMNYIGQKKLGYDQENVVIIPLKSERLRERGQLLKAEFGNLPAVSLASLTSSIPGEGL
                     DGRGFSPEGIDSKSPWILYTVRADVDLVDVLGIKIKEGRKLSRDYGTDSTAAVINETL
                     VKKLGWDDPIGKKIYDFGQGDSARTFFTVVGVAEDYHFKSLHDPIEPSIIYINREDAP
                     FLALKLHPGNTEAAIEQLRLKWEEVESAFPFDYFFMKEQYGDLYKREQKMAGLFITFT
                     VLAMVIACLGLFGLALFSIEQRTKEIGIRKILGSSVFSIMYRLSLEFSKWIVVASVIA
                     WPVAWLLVDKWLQSFAYRIEIGHFAWIFIASAVIALVIAQTTILYHAYRAATRNPVDA
                     VKWE"
BASE COUNT          977 a         1061 c         1019 g          974 t
ORIGIN      
        1 tccgcattcc gtaatttgct gagaaacaag ttctatgctt ttctcaacat actcgggcta
       61 tcggttggcc tggccgcttt catcttcatc ctgatctatg tcctggatga gatcaattac
      121 gacaaacaca acgaaaaaca tgcccgcatc taccgcatcg aatcggattt taatatatcc
      181 aaccgtcacg accggttcgc tatcgtcccc gttcccatgg ggcctgcatt caagctggag
      241 ttcccggagg tggaggccgt tacccgcctg aacccggtcg gtaatgccct gttccgatac
      301 ggagaaaagg aatactatga ggatgatttc tatttcgccg attccaatat cgccgaagtg
      361 tttacgctta attacctgtc gggcgtgccg gagaaggccc tgaacgagcc tttcaccatg
      421 gtgctgacaa aaaagatcgc gaagaaatat ttcggcgatg cgaatcccat gggcgagatc
      481 atcaccaccg gatcaggccg gtcatacaag gtgacaggcg ttatcgaaga tctgccggcc
      541 aattcgcatt tgcgttacgg cgccctgctt tcaaccgttt cactggccga actctacggg
      601 cgcgacaact ttaacagcat ggagccgctg gcattctgga atatcggcgt attcaccttc
      661 atcctgttga acgaaaatgc cagtatggaa aatgtcgtcg aaaagtttcc gtcattttac
      721 gaaaagtaca tgaaaccgat cggcgaccag atcagcgcca gcttctcgct gctctatacc
      781 ccgctggcca aaacccactt tgcgcagggg ctcggctccg agctgccgac aggaaacatg
      841 gcctacatct acattttctc tgctgtcgct ttcttcatcc tgctgctggc caccatcaac
      901 tacatgaaca tggctaccgc ccggtcggcc aaccgggccc gggaagtggg catgcgcaag
      961 gtggtcggcg cctaccgcaa ccagatcatc agccagttcc tctccgaatc ggtgatcatg
     1021 gccctcatgg ccctggtcat cgccttgtgc atcgttttnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnngc agcccaggta ctttatgacg gcccgtatta aagagggccg
     1201 ggaaaaagag gcgctggcat acatcggcga caagtggaat accttccagg caggaaggcc
     1261 gttcgattac aaatatgtgt cacaaatcct ggaagaacaa tacggaggtg agaaaaaaac
     1321 cgggatcata ttcaatatag ccaccatcat aacgattttc atcgctttgc tcggattgct
     1381 gggactctct tcgttcgtca cggaacaacg caccaaagag atcggtatcc ggaaaattct
     1441 cggagcaacc gtccccggaa tcctgaattt gctctaccgc gaattcgtca tattgatcct
     1501 tattgcattc gtactggctg tgcctgtagc ctggtggcga ctagacatct ggttgaacga
     1561 cagctttatc tatcatacca gcctgaactg gacctacttt ctgctggccg gaggactcgc
     1621 ctttgcggtg gggatcatga ccatcagctt ttatattatc cgggctgcta acagcaatcc
     1681 ggtagacgcc gttaaatggg aataaaatca tcttaaaatg tatgaacatt acctgaagac
     1741 aagcctccgg aacttctggc gctaccgggg atacaccctg atcaacatgc tgggtcttgc
     1801 aacgggtata gccacctgcc tgatcatttt cacctatatt tcgcatgaac ttagttatga
     1861 taagtttcat gaagattatg aagatgttta ccgggttgcg gtaaaaggcc gttttgccga
     1921 agactttttc aatgtggcct gctctatgcc cccgatcgga ccgacattga aagaaaagtt
     1981 ccctgaagtg atggccttta cccggatcga taaattccag aacaatcctt tcctggctta
     2041 tgaagataaa aagttctatg aggatgggct ctactttgcc gattcggcat tcctggatgt
     2101 tttcactttt gagatgataa acggagaccc ggcaacagcg ctcgtggaac cgaactgtat
     2161 cgtgctgacc gaagggatcg ccgataaata ttttgaaaat gaaaacccgg tcggcaaagt
     2221 gcttcggctg aacgaccagg tcaatatgaa agtgaccggt gtgttgaggg acataccgga
     2281 aaacacccac ctcaaatttt ccatgatcgg atcgataagc acggtcaatg gccgattggg
     2341 gagggagccc atggatgaaa actggggatc gctatttctg cacaactata tccggctcta
     2401 tcccggcacc ggtgcagcgg atttcggcga aaaaatccgc tacgttatca aagatgcttt
     2461 cggagaagca gcggaaacgt ataacattga gatgatcccg tatcttcagc cactggcgtc
     2521 catacacctg tattccaacc tgatggccga gctggaaccc ggcagcgaca tctcgtacat
     2581 ttacatcttt gccgccgttg ctctttttat cctgatcatc gcctgtatta atttcatgaa
     2641 cctgaccacg gcccgttcca ccaaacgaag ccgggaagtg ggcatgcgga aagtggcggg
     2701 cgccacgcga aaacaactga tagggcagtt cctcagcgag tcgatgctga tcagcctgct
     2761 gtgcatggta tttgccttca ttacggccga ggcggtcctg ccctttttca gcgacctgac
     2821 cggtctgaac ctcgaaccct ggttccacac acccgtcatc atcccggtca tcctggggct
     2881 ggggctgttt gtcggggtat tcgccggcgc ctatcctgcc ttcattctct catcgttcca
     2941 gcccatccgg gccatccggg gagaactcta caggggaatg aagaaatcgg tgctccgcaa
     3001 cctgctcgtg gtgcttcagt ttgctatctc cattgccatg ctggtaagta catggctcat
     3061 ttacaagcag atgaactata tcggacagaa gaaactgggc tacgaccagg aaaatgtggt
     3121 catcatcccg ctgaagtcgg agaggctgag ggagcgcggg caactgctca aagccgaatt
     3181 cgggaacctg ccagccgtca gcctggccag cctgacctcg tcgataccgg gagaaggcct
     3241 tgacggcagg ggcttttccc ccgaagggat cgacagcaaa tcgccgtgga tcctttacac
     3301 ggtcagggcc gatgtcgacc tggtggatgt gctgggaatt aagattaaag aaggaagaaa
     3361 gctctccagg gattatggca ccgattccac ggctgcggtc atcaacgaaa ccctggtgaa
     3421 aaagctcggc tgggatgacc ctatcgggaa gaaaatctat gacttcggac agggcgacag
     3481 cgcccggaca ttttttacag tggttggagt agctgaggat taccatttca aatcgttaca
     3541 cgatcccatc gagccctcca ttatttacat caacagggaa gatgctccct tcctggctct
     3601 gaaactccat ccgggcaata cggaagcggc tatcgaacaa ctccggctga aatgggaaga
     3661 ggtggaatct gcttttccgt tcgattattt tttcatgaag gaacaatacg gcgatttgta
     3721 caaaagggaa cagaaaatgg ccggtctctt tatcacattc acggtactgg cgatggtcat
     3781 tgcctgcctc ggcctgtttg gcctggcgct tttcagcatc gaacagcgga ccaaagagat
     3841 cggtatccgg aagatattag gatcttcggt tttcagcatc atgtaccgcc tttcgctgga
     3901 attcagcaaa tggatcgtgg tcgcgtcggt aatcgcctgg cctgttgcct ggctcttggt
     3961 cgacaaatgg ctgcaatcct ttgcctaccg gattgagata gggcattttg cctggatttt
     4021 cattgcatcg gccgtcatcg cgctggtcat tgcccagacc acgatactct atcatgctta
     4081 ccgcgccgca acacgcaatc cggttgacgc ggtgaagtgg gaatagggaa t
//
LOCUS       DAOPNH010000288         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_25697_length_4113_cov_5.197634, whole genome shotgun sequence.
ACCESSION   DAOPNH010000288 DAOPNH010000000
VERSION     DAOPNH010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25697_length_4113_cov_5.197634"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            959..1927
                     /locus_tag="PKZ74_12685"
     CDS             959..1927
                     /locus_tag="PKZ74_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93657.1"
                     /translation="MTGSVLGPETTMPLYAEGLAIKTNWTGYYLPVVQSPFDAIGEEL
                     LEDMNMLKGQYWSCFKKDPGHTKTTGSIWGCACQQGRVEIKYGDMIEIYPTEDIPEFH
                     WQMAWMQQESEPKPPSLLFQYEEKAEYDALFIELDTLNLPEEIGAFAGDSCIGATTVL
                     PSDTMALICAYGEGFEGEEITFELLYPTKSARPRCRDYSVLNTTTGIQEKRRIVAGEN
                     QPYFLVSFKSQENIPAKETAFNVIVRPNPAGNEFTVSYFTGQEALVELSLINALGLPV
                     RSWQRGIHGAGSYSMAISTAGLPSGCYYLKVKAGNAVEIQKIMIIH"
     gene            2103..>4113
                     /locus_tag="PKZ74_12690"
     CDS             2103..>4113
                     /locus_tag="PKZ74_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPM93658.1"
                     /translation="MKIKNIIVIFWFMILLINQVYPQVITVKQDGTGDFTIIQEAVDI
                     ANDGDTVIVYPGVYFENVDLTEKDIVLASTWLLFGQDSLISQTIIDGNQLGSCIKTKS
                     GSEWSEVLGFSLQHGVGTKHGDLQPELYGYGGGIYISKSKLEVTRCKITDNFGMYGGG
                     IVALESSVRFTGNNIHNNWAAGGGGGILTSNSIVEFDSIMLNSIYLNYGSFATDIAVK
                     LNDSLQKIWLDTCTVLNPDQYYIGRFENHAIHVSRPPISVLNGMIEQVNADLFVSTSG
                     DDSNSGLTIGDPLKTISFALLKISSDSLDPKTVHVTDGIYSPSQTGEHVPIQLKNHVN
                     LVGQSRENTVIDCEVKYEGARFAFGQDYSMAKNISFVNGNGYPTWLNGGISTGYSKKI
                     VLDSIAIINATGDHSVGIYSDSGDSIIIKNCIFENCAGYCPVEIFTGYQSPPTYAELV
                     SNRFSLNHPDTNAEWEFRQVTLFFVGSISTPGKIMAKVVNCLFNDNSDSTVFAPDPVP
                     IALLALYGVFVEMANCTFANNTTTNDLGAALSLAYGSHANLYNCIFFGNVPKQATLAN
                     TPDEPSTLGIYNSLIQDGLDGIYNNGGVNFIEWGAGNLDANPIFFGSGEHPYSIDYGS
                     PCIDAGTTDLPPWIDLPEYDIAGNPRVYGESVDMGAYEYGPWVNVP"
BASE COUNT         1216 a          894 c          919 g         1084 t
ORIGIN      
        1 tgagcattat gaaataattg atttcgaaat attgaataat ggagattgta tggccctggc
       61 gacagaaaat gggatttatg gttgtaaact tttaaagata gataagaata caaatgaaat
      121 agttgcaacc ttgccattgt caagaacacc tgtcggacat agagatggtc ttttttataa
      181 tagctctaaa aatcaacttt atcttctatc agaatattat ttaacagtca ttgatccatt
      241 aacactccaa caaataagtg aaattacaca acccgaagat gatttatttt taaaatgcca
      301 ctattcacca agaagcgaaa aagtattggt gatttgtaaa aataatcgga aagtactcag
      361 ctacaatgcc aataattttt cattaaataa tcaattcgag ttgccggtaa atgctatcat
      421 caaatcttca ttttataaca gtattaatga tttgctttac ctgcatcata ctaaatatga
      481 tctgaatctt tacaaaaatg aaataagact cttatcaata agatgtgacg atttccaggt
      541 tcaaagtgat atttatttac agcaaaatac tcttaaaaat gtattttcgg cgactctgac
      601 agcacaaata gtctgggatg aatccaccaa taccattttt atacctaatg agggattagg
      661 taatattagt aaagtaaact gttctgatga ccagctgatc ctccagcccc gcacctggaa
      721 ctggatctct ttcccgcggc ttgaaagaga aggtaacgga gaggtttcta ccgtggagca
      781 gttggaaaat atcgctcctt ttccagataa tattacaatg actcgttggg tacctttagg
      841 tcagggaaca attgataaag tatggaatgg aattatttgg actggaaact taaatacagt
      901 tcagagcaca ttaggctata agctggagac caataatcct ggggtttcaa tacttcccat
      961 gacaggaagc gttctcggcc cggagacaac gatgccgcta tatgccgagg gcttagccat
     1021 aaaaacgaac tggaccggct attatctgcc ggtggttcaa agccctttcg atgccatcgg
     1081 ggaagaattg ctcgaggata tgaatatgct caaaggccaa tactggtcgt gctttaaaaa
     1141 agacccgggg cataccaaga caaccggaag tatttgggga tgtgcctgcc agcagggaag
     1201 ggtcgagatc aaatacggtg acatgatcga gatctatccc acagaagata tccccgaatt
     1261 ccactggcag atggcctgga tgcaacagga atcggaacct aaaccgcctt ccctgttatt
     1321 ccagtatgag gaaaaggcgg aatacgatgc ccttttcatc gaactggata ccctgaacct
     1381 gccggaagag atcggcgctt ttgccggaga ttcctgtatt ggtgcaacta cggtattgcc
     1441 ctccgacacc atggccctga tctgcgctta cggcgaagga tttgaaggtg aagagatcac
     1501 cttcgaactg ttgtatccga ccaaatcggc caggccaagg tgcagggatt attctgtatt
     1561 gaataccacc actggaatac aggaaaaacg caggatcgtg gctggagaaa accagcccta
     1621 tttcctggtt tctttcaaat cgcaggaaaa tattccggct aaggagacgg cgttcaatgt
     1681 cattgtccgg ccaaatccgg ctgggaacga atttaccgta tcgtacttca ccgggcaaga
     1741 ggcgttggtc gagttgtcgt tgatcaatgc gttgggattg ccggtcagaa gctggcaacg
     1801 aggaatccat ggcgcgggga gctattcgat ggcgatcagc acggccggtt tgccttccgg
     1861 ttgttattac ctgaaggtca aggccggtaa tgctgttgaa atacaaaaaa ttatgatcat
     1921 tcattaacat ttaacgggag tgcatcaaaa gtctttaata aaccacaaag gcgcacaaaa
     1981 tatatcacaa aggcgcgcaa agtacctgat tacaggatta aaacctttgt gcgccttcgt
     2041 gcaacccttt gtgtgccttt gtggttaagc actttcgaga aatcctcatt aaaaaaagag
     2101 cattgaaaat taaaaatata atagttatct tctggtttat gatcctgttg atcaaccagg
     2161 tttatcccca ggttatcacc gtcaaacagg acggaaccgg cgatttcacg ataatacaag
     2221 aagcggttga cattgcgaac gatggggata cggttatcgt ctatccggga gtctacttcg
     2281 aaaacgtgga cctgaccgaa aaagacattg tcctggcaag tacatggctc cttttcggtc
     2341 aggatagcct gatctctcaa accatcattg acggcaacca actgggaagt tgcattaaaa
     2401 cgaaatcagg gagcgagtgg tcggaggtgc tcggattctc cctgcagcac ggcgtgggga
     2461 caaaacatgg agacttgcaa ccagagctgt atggatatgg aggaggtatt tatataagca
     2521 aatctaaatt agaagtgacc agatgtaaga ttacagacaa ttttgggatg tatggcggag
     2581 gaattgtcgc gctggaatcc tcggtaagat ttacaggaaa taatatacat aataactggg
     2641 ccgcgggtgg aggaggagga attctcacct caaactcaat tgttgaattc gatagtatta
     2701 tgttaaacag tatctatctg aattatggat ctttcgcaac tgatatagcg gtaaaactca
     2761 atgactcgct ccaaaagatc tggcttgaca cttgtacggt actcaatcct gatcaatatt
     2821 acatcggccg gttcgaaaat cacgccatcc atgtcagccg tcccccgatt tcggtgctaa
     2881 atggaatgat tgagcaagtc aatgccgacc tgttcgtcag tacatcaggc gatgattcca
     2941 attcagggtt gacgatcggc gatccattga aaaccatctc ttttgctttg ctaaaaattt
     3001 cttccgacag ccttgaccct aagacggtcc atgtaaccga tggcatatat tccccatccc
     3061 agacaggaga acatgttccc atccaactta aaaatcatgt aaaccttgtt ggccaaagcc
     3121 gggaaaacac cgttattgac tgcgaagtta agtatgaagg cgcacgattt gcattcgggc
     3181 aggattacag catggctaag aacatctcct ttgttaatgg gaatggttac cccacgtggc
     3241 ttaacggagg catatcgaca ggttattcta aaaaaattgt attagatagt attgcgataa
     3301 tcaatgcaac cggtgatcac tccgtcggaa tctactcaga ttcaggcgac tcaattatta
     3361 ttaagaactg tatctttgaa aactgtgcag ggtactgccc agtcgagatt tttaccggtt
     3421 atcaaagtcc gccaacttac gcagaactgg tctcgaaccg cttttccctg aaccatcccg
     3481 atacaaacgc agaatgggaa ttcagacaag ttacattatt ctttgtagga tcaatttcca
     3541 ccccaggaaa gattatggca aaggttgtta attgcctgtt taatgataat tccgacagta
     3601 cggtcttcgc ccctgatccg gtgcccattg cgttattggc actttacggt gtgtttgttg
     3661 aaatggccaa ctgcaccttt gccaacaaca ccaccactaa tgatcttggc gccgccttga
     3721 gccttgccta tggttcccac gccaatctgt acaattgcat tttttttggt aatgtcccaa
     3781 aacaagccac cctggccaat accccagatg agcccagcac actgggaatt tacaactccc
     3841 tcatccagga cgggctggac ggcatatata ataacggcgg ggtgaatttt atcgaatggg
     3901 gagcaggcaa cctggatgca aatcccatat ttttcggctc gggagagcat ccttattcca
     3961 tcgattacgg ctctccctgc atcgacgccg gcaccacaga cctgccgcca tggatcgatc
     4021 tacccgaata tgatatcgca ggcaatcccc gcgtttatgg agaatccgtt gatatgggag
     4081 catacgaata tggcccctgg gtcaatgtgc ctt
//
LOCUS       DAOPNH010000289         4081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_25949_length_4081_cov_5.829111, whole genome shotgun sequence.
ACCESSION   DAOPNH010000289 DAOPNH010000000
VERSION     DAOPNH010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25949_length_4081_cov_5.829111"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1611)
                     /gene="lepB"
                     /locus_tag="PKZ74_12695"
     CDS             complement(97..1611)
                     /gene="lepB"
                     /locus_tag="PKZ74_12695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPM93659.1"
                     /translation="MNIFLLLFIIFFISSLVGLWKIFIKAGYPGWTVLVPFYNFYIWL
                     KIIKKPLWWYIFLLIPFINVFTILLMIVELLKCFNKFGLGAQALGVLFPFAYLPYLGF
                     TEKEKYIHPDKRKEFKKTWLREWVDAIIFAVIAATIIRTFLIEAYTIPTPSMEKTLLV
                     GDFLFVSKMSYGPRVPMTPLSFPFVHHTLPMTTETKSYLEWIKLPYYRFPGFGKVQRN
                     DAVVFNYPDGDTLSVKFQSNRSYYSMVREYGWSRVNRNSREFGEIISRPVDKRENYIK
                     RCIAIAGDTLQIIDQQVYINGEKAFNPGITEYQYLIKSNISQLNPRLLDELDVTDKVW
                     PVGPGEFVTTLTDKAANELRRVKGIESVEVLMEPAGKWDRETFPYSGDYPWNRDNYGP
                     IVMPKAGTSIDLTLKNLPLYERIIKNYELNDLKVTDGRIYINGVESSQYTFKMNYYWM
                     MGDNRHNSADSRYWGMVPEDHIVGKAVFVWFSMDKNKSLFDGKIRWNKLLRTVK"
     gene            complement(1698..2420)
                     /gene="dapB"
                     /locus_tag="PKZ74_12700"
     CDS             complement(1698..2420)
                     /gene="dapB"
                     /locus_tag="PKZ74_12700"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPM93660.1"
                     /translation="MNIILSGYGRMGHEVEKAAIARGHRIVARIDTEDDWATCGPLFS
                     SADVVIDFSRPASAVANIRHCFDHHIPVVTGTTGWNKDEPLVRKWCADENQSLFVGSN
                     FSIGVNILFALTGKLGELMNRFDEYNISLEEIHHIHKLDAPSGTAIKLAEMILSRVER
                     KKSWVNQTERTPEELQVISVREGEVPGTHSIVCDSEADRITLRHEAKNRKGFALGAVM
                     AAEWLPGKPGYFDMNDLLGLTL"
     gene            complement(2510..3001)
                     /locus_tag="PKZ74_12705"
     CDS             complement(2510..3001)
                     /locus_tag="PKZ74_12705"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPM93661.1"
                     /translation="MNLTFPDKNLILSFLAVLFLILACKTAASQDTTKTRREPAKHSV
                     GKAAIFSAVLPGLGQAYNRKYWKIPVVYAGFGAIGYFVVTNDKQYDTFKEAYLYVANA
                     DTTPIDNPYVTKYSKDQLRSSMEYYRRNRDLSIIIGALWYAVVILEAYADAHFLIMTS
                     AKT"
     gene            complement(2998..3885)
                     /locus_tag="PKZ74_12710"
     CDS             complement(2998..3885)
                     /locus_tag="PKZ74_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPM93662.1"
                     /translation="MTSKKKALGRGLDAILQSPETDITSRDISGDYVAGAVAEIEIGK
                     IEANPFQPRTDFEAESMEELARSIGEQGIIQPLTVRKAGFDRYQLIAGERRLKASKMA
                     GLSTVPCYIRVANDEQMLELALIENIHRKDLNAIEIGISYQRLIEELNLTQEEVSKKV
                     GKDRATISNYIRLLKLPVEVQYALRNDQISMGHARTLIGISDEEKQLKTLLKIVEKGL
                     SVREAEKLAREIQAEPAVKEPALSSLPEHLQEAGLKLHEKIGAAVRIKSNTKGAGSLV
                     IKFGSGNELERILKLLNES"
     gene            complement(3875..>4081)
                     /locus_tag="PKZ74_12715"
     CDS             complement(3875..>4081)
                     /locus_tag="PKZ74_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93663.1"
                     /translation="FQQMVFDTIIHRNTRLSEAPSFGETVIMHDATSIGAINYLNLAR
                     EILQKNGMTHLSEADKTIDIVNDI"
BASE COUNT          951 a          980 c         1008 g         1142 t
ORIGIN      
        1 aaaagttgca aaagaagtca gcataaatac ggctgccagc agccaggtta acatagaact
       61 ttttttcatt ttttaattta tttaggtaaa acttaattac ttgacagtgc gcaaaagctt
      121 gttccaacgg atcttgccat cgaagagcga tttgttctta tccatagaga accatacaaa
      181 gaccgctttt ccgacgatat ggtcttccgg gaccatcccc cagtagcggc tgtcggctga
      241 attgtggcgg ttatcgccca tcatccagta ataattcatc ttaaaggtgt attgtgagga
      301 ttccacaccg ttgatataaa tcctgccatc cgtcactttc aggtcattca gttcgtaatt
      361 cttaatgatc cgttcgtata agggtagatt ttttagtgta aggtcgatgg aagtacccgc
      421 ttttggcatc acgatcggtc cgtaattgtc acggttccag ggataatcgc cggagtaggg
      481 gaaagtttcc ctgtcccatt ttccggccgg ctccatgagc acctccaccg attcgatccc
      541 tttgactcga cgcagttcgt ttgcagcttt atcagtcagc gtagtaacga actctccggg
      601 ccctaccggc cagaccttgt cggttacatc cagttcgtcg agcaaccgtg gattaagctg
      661 gctgatgttc gatttaatca ggtactggta ttcggtgata cccgggttga aggctttctc
      721 gccgttgata taaacctgct ggtcgatgat ttgaagcgta tcgcctgcta tggcaataca
      781 gcgtttgatg taattttccc ttttatcgac cggcctggaa atgatctctc cgaattccct
      841 tgaattccgg ttcacgcgcg accagccgta ttccctgacc atggaataat aactccggtt
      901 gctctgaaac ttaacggaaa gtgtatcgcc atccggataa ttaaagacga ccgcatcgtt
      961 tctttgtact ttgccgaatc ccgggaaccg gtaatagggc agtttgatcc attccaggta
     1021 ggatttggtc tccgtggtca tgggcagggt gtgatgaacg aacgggaagg agaggggggt
     1081 catggggacg cggggcccgt agctcatttt actcacaaac aggaaatccc cgaccagcag
     1141 ggttttttcc atggagggcg ttgggattgt gtaggcttcg atcaggaatg tgcggatgat
     1201 cgtagcggcg atgacagcaa aaatgatcgc gtcgacccat tcgcgaagcc aggttttctt
     1261 gaattctttc cttttgtcgg gatgaatgta tttttctttt tccgtaaacc cgagataggg
     1321 gagatatgcg aaggggaaaa gtacgccgag tgcctgggcg cccagtccga atttattgaa
     1381 acacttcaac agttccacga tcatcagcag gatcgtgaat acattaataa atgggatgag
     1441 gagaaaaata taccaccaga gcggtttttt aatgatttta agccagatgt agaaattgta
     1501 aaacggaacc agcaccgtcc atccgggata tcccgcttta ataaagattt tccataaacc
     1561 aacaagggaa ctgatgaaaa aaatgatgaa aaggaggagg aaaatgttca ttgagttgtg
     1621 atttggttgc gaaagtaacg aataaatgga aaaccgttgc gggacttagc tgttaatttt
     1681 tgttaagccg gtgaaaatta tagagtaagc ccgagaaggt cgttcatgtc aaaatatcct
     1741 ggcttgcccg gaagccattc tgcggccata actgctccga gggcaaatcc tttcctgttt
     1801 tttgcttcat gccgaagcgt gatccggtcg gcctccgaat cgcagacaat agaatgggta
     1861 cctggtactt ccccctccct gacagatatc acctgcaatt cttcgggagt acgttcggtt
     1921 tggttcaccc atgacttttt ccgctccacc cggctcagga tcatttcggc cagtttgatt
     1981 gccgttccgc ttggcgcatc aagcttatgg atgtggtgaa tctcttccag tgaaatattg
     2041 tattcatcaa accggttcat caattcgcca agtttgccgg tcagtgcgaa caggatattt
     2101 accccgatgc tgaaattgct tccgacgaaa agggattgat tctcatccgc acaccatttt
     2161 ctgacaaggg gttcgtcttt attccagccg gtcgttccgg ttaccacggg aatatgatga
     2221 tcgaagcaat gccggatgtt ggcaacggca gatgcgggcc ggctgaaatc gatcactaca
     2281 tcggcgctgc taaacaacgg gccgcaggtc gcccaatcgt cttcggtatc gatccgggca
     2341 acaatccggt gcccgcgggc gatagcggct ttttcgacct catggcccat tcttccgtat
     2401 cctgaaagga ttatattcac tgtttaaaac tttaaggtaa tatgtatgcc cggtaccggc
     2461 tgacgtggcc gttccggtga tggaagcgct gccgggccta ctttcagcgt caggtcttcg
     2521 ctgacgtcat aatcaaaaaa tgtgcatcgg cgtaagcttc caggatgacg actgcgtacc
     2581 ataacgcccc gataatgatc gacaggtcgc ggttacggcg gtagtattcc atcgagctcc
     2641 tgagctggtc tttggagtat tttgtgacat atggattatc gatcggcgtc gtgtcggcat
     2701 tggccacata caggtacgct tctttgaatg tgtcgtattg cttgtcgttg gtcaccacaa
     2761 aataccctat cgccccaaaa ccggcataca caacaggtat tttccagtat ttccggttgt
     2821 aagcctgacc caaccccggc aagacggccg aaaaaatggc tgctttgcca acagagtgtt
     2881 ttgccggttc tctcctggtc tttgttgtat cctgggaagc tgctgtttta caggccaata
     2941 tcaaaaataa tacagccaga aaggagagaa ttagattttt atcgggaaaa gtgaggttca
     3001 cgattcgttc agaagtttta aaattcgctc cagttcatta ccggatccga atttaataac
     3061 gagcgaaccg gcccctttcg tattactttt gattcttacg gccgccccga ttttttcatg
     3121 gagtttcagt cccgcttcct ggaggtgttc cggaaggctg ctcaaagctg gttcctttac
     3181 ggccggttcc gcctggatct cgcgggccag tttttccgct tcccttaccg ataatccttt
     3241 ttcgacgatt ttcagcagcg tcttcaattg cttctcttca tcggaaatgc cgatcagggt
     3301 gcgcgcatgg cccatggaaa tctggtcgtt gcggagagca tactgcactt caaccggaag
     3361 tttcagcaaa cggatgtagt ttgaaatggt ggcgcggtct tttccgacct ttttactgac
     3421 ctcttcctgg gtcaggttca gttcttcgat cagacgttgg taactgatgc cgatctcaat
     3481 ggcgttcagg tctttccggt ggatgttctc gataagcgcc agttccagca tctgctcatc
     3541 gttagctacg cggatataac aaggtaccgt agaaagccct gccatttttg aagctttcag
     3601 tctccgttcg ccggcgataa gctggtagcg gtcgaatccc gcctttctta ccgtaagagg
     3661 ctgaatgata ccctgttcgc caatgctgcg ggcaagttct tccatgcttt ccgcttcaaa
     3721 atcggtacgg ggctgaaacg ggttggcttc tatctttccg atctctattt cggcaacggc
     3781 acctgccacg taatcgcccg agatatcgcg ggatgtgata tcggtctcgg ggctctgcag
     3841 aatggcatcc aatccccggc caagcgcttt tttcttagat gtcattaact atatcgattg
     3901 ttttgtcagc ttcactcaga tgggtcattc cgttcttctg gaggatttcc cgggccaggt
     3961 tgagataatt gatcgctccg atactggtgg cgtcgtgcat gatcaccgtt tcaccgaaac
     4021 tgggcgcttc gctcagccgt gtgttacgat ggataatagt gtcgaagacc atttgctgga
     4081 a
//
LOCUS       DAOPNH010000290         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_26091_length_4064_cov_6.161886, whole genome shotgun sequence.
ACCESSION   DAOPNH010000290 DAOPNH010000000
VERSION     DAOPNH010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26091_length_4064_cov_6.161886"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2098)
                     /locus_tag="PKZ74_12720"
     CDS             complement(125..2098)
                     /locus_tag="PKZ74_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM93664.1"
                     /translation="MSLGRQLDDKAPNYLKQALGWSYHAPYAGHDGIQLAIGQAFTVG
                     KDHLFPYYRDMLTAISAGLTAEEIILNGLSKKDDIASGGRHMSNHFAKPEWNIHNVSS
                     ATGNHTLHAVGVARAMKYYKSGGVAISSQGESSTSEGYVYEAINGASNEELPVIFVFQ
                     DNGYGISVPKEDQTANYYAATNFEGFKNLRIIYCNGLDVFSSMNAMAMAKKHVLETGK
                     PVIVHAHCVRMHSHSNSDRHELYRDDTEIDEARGRDPLQKFRKRLLFSEIFSEDELNA
                     LDEKAKKEISAAHKKVIMMPDPDPATIFDHVIPAPYIPVNFPEGLHQESGTPKKLIQA
                     LNDTLKEEFRLNPDTFIWGQDVANKEKGGVFNVTKGMQQEFGPDRVFNGPIAEDYIVG
                     TANGMSRFSDKIRIVIEGAEFADYFWPGMEQFVEMTHDYWRSNGQFVSNVTIRLASGG
                     YIGGGLYHSQSIEGALSTFPGIRIVYPSFADDAAGLLRTSIRSKGPTVFMEPKTLYND
                     PVAETFVPEGFEVPFGKARIRREGKDLSIITYGNTTHMSIQAAGKIEKELGKQVEVLD
                     LRSLIPLDKQAVLDSVKKTGRALIVHEDKVFAGFGGEIAAMITEEAFEFLDAPVKRVG
                     STFTPVGFNRILERAILPNSDKIYHAAAELLGY"
     gene            complement(2302..3090)
                     /locus_tag="PKZ74_12725"
     CDS             complement(2302..3090)
                     /locus_tag="PKZ74_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPM93665.1"
                     /translation="MKARISIIQFRPELADPAKNIGNLDNRMDEAKGSQLIVLPELSN
                     SGYNFTDASQALSVAETLDGQGAYQRFIADKAKKLNAFIVSGICEKDGNDLYNSAILS
                     GPAGIIGSYRKMHLFMNEKDIFRPGNSALQVFEAGGLKIGMMICFDYLFPEPWGILAR
                     HGADIICHPSNLLTGNPHKCMPGLALMNRIYIATANRIGTEGSLTFNGKSFFTDPAGN
                     VPVIASPDQEEIICLDINTDISSDKMITQRNHVFTDRRPEMYAG"
     gene            complement(3093..3656)
                     /locus_tag="PKZ74_12730"
     CDS             complement(3093..3656)
                     /locus_tag="PKZ74_12730"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPM93666.1"
                     /translation="MDEEINSTVSFLKKGRIILYPTDTIWGIGCDATMTKSVDQIYRL
                     KQRAEKKSMIILLDSAEKLAYYIRKVPSIAYDLLERYLEPLTIIYPGAMNVAKNVIAD
                     DGSIAIRIVRDEFCRKMIEQLGKPVVSTSANLSGQPAPLVFSKIAPEIKNGVDYIVNY
                     NRDVMVRSKPSTIIRLLGEGEFEVLRK"
     gene            complement(3686..>4064)
                     /locus_tag="PKZ74_12735"
     CDS             complement(3686..>4064)
                     /locus_tag="PKZ74_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPM93667.1"
                     /translation="PFYGDHVSLFTLLMTISTIIYTYLNNQMMGSTSQQLPGMKTMMY
                     LMPIMFLGIFNNYSSGLSYYYLLVNLITFAQMYIFRYFVDEDKLHKKIQENKLKPVKV
                     SGFQKRLEDMAKQRGYNPNTRKK"
BASE COUNT         1007 a          931 c          937 g         1189 t
ORIGIN      
        1 tcaggaataa aacggcagtc aaaagcaatc cggcgataaa atttttcttc atcttcatgt
       61 atttaaaata agaacggaaa aacagccaat ctaatataaa aagcgcctta ttaattagtc
      121 tttatcaata tccaagcaat tctgcggcgg catggtaaat tttatcggaa ttgggaagaa
      181 tggcccgttc caggatccgg ttaaagccga caggcgtaaa ggtcgatccg acccttttta
      241 cgggtgcgtc aaggaattca aatgcctctt ccgttatcat cgccgcgatt tctccgccaa
      301 atccggcaaa gactttatct tcatggacga tcaatgcgcg gcccgttttc tttacagaat
      361 cgaggacagc ctgtttatca agcggaatga gagaacgcag gtcgagaacc tcaacctgtt
      421 tacccagttc tttttctatt tttccggccg cctgtatcga catatgcgtc gtatttccgt
      481 aagttatgat gctcaggtcc ttcccttccc ttctgatccg ggctttaccg aaaggcactt
      541 caaatccctc gggcacgaag gtttcggcca caggatcgtt atagagcgtt ttcggttcca
      601 tgaaaacggt tggccctttt gagcggatgc tggtccggag caggccggcg gcgtcatcgg
      661 caaatgaagg gtagacaatt cggattcccg ggaatgtgga gagggctcct tcaattgatt
      721 gagaatgata cagtccaccg ccgatataac cgccggaagc cagcctgatc gtcacattgg
      781 aaacaaactg gccgttcgaa cgccagtagt cgtgtgtcat ttccacgaac tgttccatgc
      841 ccggccagaa atagtcggca aattcggcgc cttcgatcac aatcctgatt ttatcgctga
      901 aacggctcat tccgtttgcc gtgccgacaa tgtaatcctc tgcgataggt ccgttgaaaa
      961 ccctgtcggg gccgaattcc tgctgcatgc ctttcgtgac attgaacacc ccgccttttt
     1021 ctttattggc cacgtcctgg ccccagataa aggtatcggg attcagccgg aattcttctt
     1081 tgagggtatc gttcagagcc tggatgagtt ttttgggagt gccggactcc tggtggagcc
     1141 cttccgggaa atttaccgga atatatggcg ccgggatcac atgatcaaaa atggtagccg
     1201 gatcgggatc gggcatcatg atcactttct tatgggcagc agaaatctct ttttttgctt
     1261 tttcatccag tgcattgagt tcatcttccg aaaatatctc actgaataat aatctcttgc
     1321 ggaacttctg gagcggatca cggccacggg cttcatctat ttccgtgtca tcccggtaaa
     1381 gttcatgcct gtcggaattg gaatgggaat gcattctgac gcaatgggca tgaacgataa
     1441 ccggtttacc tgtttccagg acatgttttt tggccatggc catcgcgttc atgctgctga
     1501 atacatctaa tccattgcag taaatgatcc tgagattttt aaatccttca aaattggtag
     1561 cggcgtaata attggcagtc tggtcttctt tcggaaccga tatgccgtag ccgttatcct
     1621 ggaaaacaaa gattacgggc agttcttcat tgctggcccc gttaatggct tcataaacat
     1681 acccttcgga ggtcgaagat tcgccttgcg aactgatggc cacgccgccc gatttataat
     1741 atttcatggc cctggccaca ccgacggcat gcagggtgtg atttcccgtt gcggaggata
     1801 cattatgaat attccattcc ggtttggcaa aatgatttga catatgccgg cctccgctgg
     1861 caatatcgtc tttttttgag aggccgttga gaatgatctc ttctgcagtc agcccggcag
     1921 atatggcagt cagcatatcc ctgtagtacg ggaacaaatg gtctttacca acggtgaaag
     1981 cctggccgat agcgagctgg atgccgtcgt gccctgcata tggcgcatgg taggaccatc
     2041 ccaatgcctg tttcaggtaa ttcggggctt tgtcgtccaa ctgccgtcca agcgacatca
     2101 gataatacca tttttggaga gtttccctgc ttactttttt aattgaatag agcttttctt
     2161 ccttaccgat ccttaatcgt ttattttgat acatacgatg agatacttaa acttgtttta
     2221 taacaatccg agggatggaa tgttcggcaa aattacacaa taattggtta ttatttgtaa
     2281 atgatctaaa tatgagatga actatccggc gtacatttca ggccgtctgt cggtgaaaac
     2341 atgatttctt tgagtgatca ttttatcgga tgaaatatcc gtgttgatat ccaggcaaat
     2401 gatctcctcc tgatcggggg atgcaattac aggaacattc ccggcaggat cggtaaaaaa
     2461 tgatttgccg ttaaacgtaa gtgatccttc tgtcccgatc cggttggcgg tggcgatata
     2521 tatccggttc atcagggcca ggcccggcat gcatttatgg ggatttcccg taagcaggtt
     2581 ggaagggtgg caaataatat cagcgccatg gcgggccagt ataccccagg gttcggggaa
     2641 gagatagtca aaacaaatca tcataccgat ttttagccct ccggcttcaa aaacctgcaa
     2701 tgctgaattc ccgggccgga atatatcctt ttcattcatg aacagatgca ttttccggta
     2761 actgccgatg atacctgctg gccctgacaa gatggccgaa ttatagagat cgttcccgtc
     2821 tttctcacat atgccactaa caatgaatgc attcagtttt ttggctttat cggcaataaa
     2881 ccgttgatat gcgccttgtc catccagtgt ttcagccaca gatagggcct ggctggcatc
     2941 agtaaagtta tatcctgaat tcgacaattc gggaagaacg atcagttggc tcccctttgc
     3001 ttcatccatg cgattatcga gattaccgat atttttggcg gggtctgcca gttcagggcg
     3061 aaattgaata atggatatcc tggctttcaa tgttattttc ttaatacttc aaattcacct
     3121 tcaccgagta agcggataat ggttgagggt ttacttctga ccattacatc cctgttgtaa
     3181 ttcacaatat aatccactcc atttttaatt tccggagcta ttttactgaa taccagtggc
     3241 gccggttgtc cggagagatt ggcggatgta gaaacaaccg gctttcccaa ttgctcgatc
     3301 attttcctgc aaaattcgtc gcgtacgatc cggatagcga tagaaccatc gtcggcgatg
     3361 acatttttcg ctacgttcat agcgcccggg taaataatcg tcagcggttc caggtatctt
     3421 tccaacaggt cgtaggcaat cgacgggact ttccggatgt agtaagccag tttctcagcg
     3481 ctgtcgagca ggatgatcat gctttttttc tctgcccttt gtttgaggcg gtatatctga
     3541 tcgactgatt ttgtcatcgt agcgtcgcag cctattcccc atatcgtatc ggttggatac
     3601 agtatgatcc tacctttctt caggaagctg acggtactgt taatctcttc atccatagag
     3661 cattcagggt caaatcaaag aattcttact ttttacgtgt gtttggatta taccctctct
     3721 gtttggccat atcttccagt cgtttctgaa atcctgagac tttaacgggt ttgagtttat
     3781 tctcctggat ctttttatgc aacttatctt catctacaaa ataacggaag atatacattt
     3841 gagcgaatgt tatcaaattg accagcaaat aataataact cagtcctgaa gagtaattat
     3901 tgaatattcc gaggaacatg atgggcatca ggtacatcat ggttttcatg ccggggagtt
     3961 gctggctggt tgagcccatc atctggttgt tcaggtatgt ataaatgatg gtagaaattg
     4021 tcatcagcag ggtaaaaagg ctcacatgat ctccgtaaaa ggga
//
LOCUS       DAOPNH010000291         4060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_26139_length_4060_cov_5.768539, whole genome shotgun sequence.
ACCESSION   DAOPNH010000291 DAOPNH010000000
VERSION     DAOPNH010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26139_length_4060_cov_5.768539"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKZ74_12740"
     CDS             <1..387
                     /locus_tag="PKZ74_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPM93668.1"
                     /translation="NIDELGTRFPDMSQAYSREINAKITAIAERNGITLHKGVYVAVT
                     GPNLETRAEYRFLSRIGADVVGMSTTPEVIVANHMKLPVAAISVITDECDPDHLEPVD
                     IDKILYYASQAEKGLVKIISDLVEEL"
     gene            397..1881
                     /locus_tag="PKZ74_12745"
     CDS             397..1881
                     /locus_tag="PKZ74_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPM93669.1"
                     /translation="MPDILTVTSPYDRHFIREFPLADEKEIEKTLAVAHGLFRDPSKW
                     LPKYQRIAILEKLATVMESQVEELTMMAAEEGGKPYKDSKVEVLRAINGVKLAVAEMY
                     HLRGEEIPMGMTAASVNRLAFTTREPIGVVLSISAFNHPLNLVVHQVIPALAAGCPVI
                     VKPASTTPVSCFNLVRLLKGCGLPDGWCTALVCRRELAEKLAADPRIGYMTFIGSHEV
                     GWKLKNLLAPGTRFALEHGGVAPVIVDGTAGIDEIVPLIAKGGFYHAGQVCVSVQKVF
                     VHRSKARELAGKLAEEADKLIVGDPLDPATDIGPMIAESEAQRVQEWVEEAISSGAEL
                     VQSSKFKVQSSKCKSKIKSSNRPIVQSSNRQIVQSPNRQIVQSPNRPIVLFAPPDSAK
                     VSREEVFGPVICVYPFDDLGDAVARANSLRYAFQAAVFTQNINTALEAAQRLNASAVM
                     INDHTAFRVDWMPFGGRDESGIGTGGIPYTMREMTREKLIVFKS"
     gene            complement(1940..3496)
                     /locus_tag="PKZ74_12750"
     CDS             complement(1940..3496)
                     /locus_tag="PKZ74_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93670.1"
                     /translation="MKRIAFILCIVSFIIYNKSKSQDYPRIYGGNFHAIVKDVHETYD
                     NGYIIAGLGQVPYGFSRFSWIIKIDINGNLIWDKKIGDWTHKYYLSGSDVTNDFGLIY
                     SGATTKYAGGYNPLFVKMDACGDIEWCKVLVSDDDNYSTGVINLQDGNYVGMLKYYGD
                     GDQYARISLVKLDQTGEPVWIQRLAQEDSLITNEEGDHLLLTSENNFLVSGRCFYPNM
                     NAYYILTDTLGNNIWDIKWNMITGISFQSTELTVGRFYSIGYCVLPGTNNLPVIFKFD
                     QSGNTLGSYLLMGDTIRGGGRSISKHNDSTLIAGFTWSEYADDNWQSEVVIIDTLGNH
                     IHRRFLLNEPDAPDDIIVTNDGKILVVVSAMVNAYVDIYLWKMNANLEDDTLYTQPLT
                     YDSLCPFEIQSDTMDLDCDLFVNIDELPTREEYESTVKIYPNPARDWVALTLPDVVAE
                     GKVEIAVYDVFGREVETGRGGDKEMEGQGEVPVNRMLVLDVSGYPPGMYVVVVTDRKG
                     RRYTGKVIVR"
     gene            complement(3524..>4060)
                     /locus_tag="PKZ74_12755"
     CDS             complement(3524..>4060)
                     /locus_tag="PKZ74_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93671.1"
                     /translation="NDQLTTHQQYLDYFDILKRIADSSWIANDLDTVSVQTLLEIKDY
                     GASAISACARGLLVKGGFLDYIETITLPQLASSEEYSYSNKIGNNSAQNKDFLKLFPN
                     PAGEYVIVYYDITSNSQLITLRIIDNQGIVIRTFLFDSSKNQVVVDLENIPNGIYFFT
                     LYIDGIQIESQKLIRLRN"
BASE COUNT         1072 a         1032 c          907 g         1049 t
ORIGIN      
        1 aatatcgatg aactgggtac ccggtttccc gatatgagcc aggcctattc cagggagatc
       61 aatgcgaaga tcaccgccat agccgaaagg aacgggatca cgttgcacaa aggcgtctac
      121 gtagccgtca cggggccgaa cctcgaaacc cgggccgaat accggttctt aagccggatc
      181 ggcgccgacg tcgtcggtat gtcaaccact cccgaggtga tcgtggccaa ccatatgaaa
      241 ctaccggtag cggccatctc cgtcatcacc gacgaatgcg accccgatca cctggaaccg
      301 gttgatatcg ataaaatatt gtattacgcc tcccaggccg agaaaggact ggttaagatc
      361 atatccgacc tggtggagga attatagctc ctctttatgc ctgatattct taccgttaca
      421 tcgccgtacg accggcattt catccgggag tttcccctgg cggatgagaa agagattgaa
      481 aagacgctcg ctgtggctca cgggttgttc cgcgatccgt cgaaatggct gccgaaatac
      541 cagcggatag cgatactgga gaagctcgca accgtgatgg agtcgcaggt ggaagagctg
      601 acaatgatgg ctgcggagga aggagggaag ccatacaaag attcgaaggt ggaggtgctg
      661 cgggccatca acggcgttaa gctggcggtg gctgagatgt accacctgcg gggcgaggag
      721 atcccgatgg ggatgacggc cgcgtcggtc aaccggctgg ccttcaccac ccgtgagcct
      781 atcggtgtgg tgctgtccat cagcgccttc aatcacccgc tgaacctggt cgtccaccag
      841 gtgatcccgg cattggctgc cggttgcccg gtgattgtca agccggcgtc gaccacaccc
      901 gtctcgtgct ttaacctggt caggttgctg aaaggatgcg gattacccga cggctggtgc
      961 accgcgctgg tttgccgccg cgagctggcc gaaaagcttg ccgccgatcc ccgtatcggc
     1021 tacatgacct ttatcggttc gcacgaagtc ggctggaagc tgaaaaacct cctggcgccc
     1081 ggcacacggt ttgccctgga gcacggcggc gtagcgccgg ttatcgtgga tggtacggcc
     1141 ggcatcgacg agatcgttcc gctcattgcc aaaggcgggt tctaccacgc cggccaggtg
     1201 tgcgtcagcg ttcagaaggt gtttgtccac cgctccaaag ctcgcgaact ggccgggaaa
     1261 ctggccgaag aagcggacaa attaatcgtt ggcgacccgc tcgatcccgc caccgacatc
     1321 ggccccatga ttgccgaaag cgaagcgcag cgggtacagg agtgggtaga agaagccata
     1381 agcagcggag cggaattagt tcaaagttca aagttcaaag ttcaaagttc aaagtgcaaa
     1441 tccaaaatca aatcgtccaa tcgcccaatc gtccaatcgt ccaatcgcca aatcgtccaa
     1501 tcgccaaatc gccaaatcgt ccaatcgccc aatcgcccaa tcgttttatt cgctcctccc
     1561 gattccgcaa aagtctcccg cgaggaggtc ttcggcccgg tgatctgtgt ttatccgttc
     1621 gacgacctgg gtgatgccgt tgcgcgcgcc aatagcctgc gttacgcttt ccaggcggct
     1681 gttttcacac agaatatcaa cacggctctc gaagcggcac agcggctgaa cgcttcggcg
     1741 gtcatgatca acgatcacac cgccttccgg gtcgactgga tgcctttcgg cggccgcgac
     1801 gagtcgggga tcggaacggg cggaataccg tatacgatgc gggagatgac gagggagaag
     1861 ttgatcgttt ttaaatcgta ggagcttgtt ttaccatgag atgccatctc gcttaaagca
     1921 gatggagtct catgaataat tacctcacaa tcactttccc cgtatatcgc ctccctttcc
     1981 tatccgttac caccaccaca tacatccccg gcggataacc cgacacatcc aaaacaagca
     2041 tgcgattcac cggtacctct ccttgtccct ccatctcctt gtctcctcct ctcccggtct
     2101 ccacctccct tccaaacaca tcatacaccg ctatctccac tttcccctca gccaccacat
     2161 ccggcagcgt tagcgctacc caatcccttg ccggattcgg gtagattttt acagttgatt
     2221 catactcttc ccgggttggc agttcatcga tattcacaaa caggtcgcaa tccaggtcca
     2281 tcgtatccga ttggatctca aacgggcaca agctatcata tgtgagtggc tgggtgtaga
     2341 gggtgtcgtc ttcgaggttg gcgttcattt tccagaggta gatgtcgacg taggcattga
     2401 ccatcgcgga aacaacaacc aggattttcc catcattagt tacgataata tcatccggtg
     2461 catccggttc atttagcaaa aacctgcgat gaatatgatt tccaagagtg tcaatgatta
     2521 ctacttcact ttgccaatta tcatcagcat attccgacca tgtaaaacct gctatcaggg
     2581 tggaatcatt atgtttagaa atacttcttc ctccacctct aattgtatct cccattaaca
     2641 ggtaacttcc aagtgtgttt cctgactggt caaacttgaa tattacagga aggttattgg
     2701 ttccgggaag gacgcaatag ccaatactat agaatcttcc aactgttagt tcggttgatt
     2761 gaaaactaat tcctgtaatc atattccatt ttatatccca aatattattg cccagcgtat
     2821 cggttaatat atagtaagca ttcatatttg ggtaaaaaca ccttccggaa accagaaaat
     2881 tattttcaga tgtaagtaag aggtgatctc cttcttcatt agtaataaga gaatcttctt
     2941 gggctagtcg ttggatccag actggttcgc ctgtttgatc caatttaact aggctgattc
     3001 ttgcatattg gtctccatcg ccgtaatatt taagcatccc aacataattt ccatcctgca
     3061 aattaatcac tcctgtactg taattatcat catcacttac caaaacctta caccattcga
     3121 tatccccaca ggcatccatt ttcacaaata aaggattata accaccggca tatttagtag
     3181 ttgcaccact gtaaatcaat ccaaaatcat ttgtaacatc agaaccagac aagtaatact
     3241 tatgcgtcca gtctccaatc tttttatccc aaatgagatt gccattaata tcaattttta
     3301 taatccagct aaagcgacta aagccatacg gtacttgccc aagtcctgca attatgtatc
     3361 cattgtcgta cgtttcatga acatctttta caatcgcatg aaaattacca ccatatatcc
     3421 gtggataatc ctgggatttg gatttattat atataataaa tgaaacaatg cataaaatga
     3481 aggcaattct tttcatgtcg aaaaattgtt ctatgaagga ttcttaatta cggagtctga
     3541 tcagtttctg agattcaatc tgaatgccat caatgtacag agtgaagaag taaatcccat
     3601 ttggtatatt ttcaagatca actacaacct gattcttaga ggaatcaaat agaaatgttc
     3661 tgataacaat accttggtta tcaattattc ttaaagtaat taattgtgaa ttcgatgtta
     3721 tatcgtagta gacgataaca tattctccag ccggattagg aaatagttta agaaaatcct
     3781 tattctgggc actattattc ccaatcttat ttgaataaga atattcttca gatgaagcta
     3841 attgaggtaa tgtgatagtt tcaatgtaat cgagaaatcc acctttcacg agcaaacccc
     3901 ttgcacaggc agaaattgca gaagccccat aatccttaat ttctaaaagt gtttgaaccg
     3961 agactgtatc caggtcgttg gctatccaac tgctgtcggc aattcgtttc agaatatcaa
     4021 aataatccag atattgttga tgagtcgtca attgatcgtt
//
LOCUS       DAOPNH010000292         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_26287_length_4042_cov_5.497116, whole genome shotgun sequence.
ACCESSION   DAOPNH010000292 DAOPNH010000000
VERSION     DAOPNH010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26287_length_4042_cov_5.497116"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PKZ74_12760"
     CDS             <1..1412
                     /locus_tag="PKZ74_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPM93672.1"
                     /translation="NNVVDLEAIMERGKRFNAEMRELDDSQAEMRTKGTAHLTNIMND
                     YDLLPTHNYKYGSHPDANAIHSEVWKARFTQGIPDGCWIGCNMACAKGVDNYELRTGP
                     YKGQKVIVDGPEYENAGGLGSNCGIFNPDYIIEANFYCDTYGICTISWGTIMAFVMEC
                     YENGILNEQRTGGLKLNFGNADSAMELLHQLARGEGMGMIAGMGVRKMKQMFIENGWG
                     DPQFLQDIGMENKGLEYSEYVSKESLAQQGGFAMTNKGPQHDEAWLIFMDMVNNQIPT
                     FENKAEALHYFPMFRTWFGLVGLCKLPWNDVEPSNNAETDEPAKVPEHVDNYVTIYKA
                     VTGKPFDKHELIRMSERVYNFQRIFNIRRGYGIRKFDAQPYRAAGPVTREEYESRADR
                     YDKQLREKLGIDPAGMPTEEKMKILRKHREEQYEKLLDAVYFRRGWTKNGVPKIEHLQ
                     KLGMDLPELIEIVKDKQGE"
     gene            1553..1930
                     /locus_tag="PKZ74_12765"
     CDS             1553..1930
                     /locus_tag="PKZ74_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPM93673.1"
                     /translation="MGRVKPSKFEIFLNYKLWLSSVTGEGIIEEDRYELLQLIKERGS
                     LKAAADEMKISYRKAWGDLKKAEELLGYELLVRQRGGKDGGQSQLTDKAIKLLAAYHE
                     LHVKMDDAVENAYEEFKKFTSIK"
     gene            2025..2972
                     /locus_tag="PKZ74_12770"
     CDS             2025..2972
                     /locus_tag="PKZ74_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPM93674.1"
                     /translation="MIFLLSCNNQPNPPRDKSTGLSGDLIIFHAGSLSYPFQKIARAF
                     EAENPGVRVLLEAAGSVDCARKITELHKPCDLMASSDYLVIQKFLIPGHTGWFLPFAG
                     NEIVLAYTDKSAYGDSISSQNWPEILMKSDVHYGRSDPDADPCGYRTLMVMQLAGDFY
                     GNQGLTESLASKDNKYIRPKEVDLLALLELNETDYIFIYRSVAVQHGLKFIKLPDEIN
                     LKITENASFYRKASVEIKGTSPGTKMMINGEPISYAITMLNESPNPGVARQFLHFMLD
                     PDKGMKILEESGHNSLIPVPYKDEFNLPGFLTKYALYQK"
     gene            3011..3997
                     /locus_tag="PKZ74_12775"
     CDS             3011..3997
                     /locus_tag="PKZ74_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93675.1"
                     /translation="MISEPARSQTQTNDFYDDAETYNLAGITKIMLDGEIGNPGTVEI
                     TAFPLRSLIIKEAVLSGDSNRFTGAYRYDGYSLYDILNHVVLKKKNEAEFPPIIDLYV
                     KVENAKGESVVFSWGEIYYPVHRHEVIIATKVARIVPSKTKDLWPLPTDIKIVAGHDL
                     ITDRNISNPLKITVLSANQSYPVNKGLSPLFSPEIKVMSDGKLLDQIDPSRDLGMKLT
                     YETIFYGRGRGIHSTMPFQGIPLKEVLKKYFKITDKNLKTGYFVVASVDGYRVVFTFS
                     EIFNRNDQAELLLIEDKDNHDGGAFRLFPAADFFSDRAVKAVSEIRFEMESQ"
BASE COUNT         1135 a          937 c         1032 g          938 t
ORIGIN      
        1 tgaataatgt tgttgacctg gaagccatta tggagcgcgg aaaacggttc aatgccgaga
       61 tgcgcgaact tgatgactca caggcagaaa tgcggacgaa agggaccgca cacctgacca
      121 atatcatgaa cgattacgac ctgctgccca ctcataatta caaatacggt agtcatcccg
      181 atgccaacgc catccattcg gaagtctgga aagcccgctt cacacaaggg attcccgacg
      241 gttgctggat aggatgcaac atggcctgtg ccaaaggcgt cgataattac gaattacgca
      301 cgggccctta caaaggccag aaggtgattg ttgatggccc cgaatatgaa aatgccggcg
      361 gactgggttc caactgcggt atcttcaacc ccgattatat catcgaagcc aatttttatt
      421 gcgatactta cggcatctgc acaatcagct ggggaacgat tatggcgttt gtgatggaat
      481 gctatgaaaa cggaatcctc aatgaacaac gtaccggagg cctgaagctg aatttcggca
      541 atgccgattc ggctatggaa ttgctccacc agctggcccg tggcgaagga atgggcatga
      601 tcgccggaat gggggtcaga aagatgaaac agatgtttat cgaaaacgga tggggcgatc
      661 cacagtttct gcaggatatc ggaatggaga acaaagggct ggaatactct gaatatgttt
      721 ccaaagaatc gctggcgcag cagggcggtt ttgccatgac caacaaaggg ccgcagcacg
      781 acgaagcctg gctgatcttc atggatatgg tcaacaacca gatcccgact tttgagaaca
      841 aagccgaagc gctgcactat ttcccgatgt tccgcacatg gttcggcctg gttggcctct
      901 gcaagctgcc ctggaacgac gtggagcctt ccaataatgc cgaaaccgat gaaccggcaa
      961 aagtgcccga acatgtcgac aactatgtca cgatctataa agcggtcaca ggaaagccgt
     1021 tcgacaagca cgaactgatc cggatgtcgg aaagggttta caacttccag cgcattttca
     1081 atatcaggag gggttacggc attcgaaaat tcgatgcgca gccctaccgc gccgcagggc
     1141 cggttacccg ggaggaatat gaatcaagag ccgatcgtta cgacaagcag ctcagggaaa
     1201 agctcggcat cgatccggcc ggtatgccta ccgaagagaa aatgaagatc ctcaggaaac
     1261 accgcgaaga gcaatatgaa aaactgctcg atgccgtcta tttccgcagg ggctggacga
     1321 aaaacggcgt gccaaagatc gagcacctgc aaaagcttgg gatggacttg ccggaactga
     1381 tcgaaatcgt aaaggacaag cagggagaat agttcccggc tgtattaggt cctggagttt
     1441 tgtcttaccg caaaatcggt cactaaatcc attgcctttt tttgacatga taatccattg
     1501 tattactttt gcaggtgata tgcacctgta tgcatatcga taattttaag atatgggaag
     1561 agtaaaaccc agcaaattcg agatatttct caactacaaa ctgtggttgt cgtcggtgac
     1621 cggagaaggg atcattgaag aggaccgcta tgaactgtta cagcttatta aagagagagg
     1681 atcgttgaaa gcagcggctg atgaaatgaa gatcagttac cggaaagcct ggggggattt
     1741 aaagaaggca gaagagctcc ttggatatga gctcttagtg cgacagcggg gaggaaaaga
     1801 cggcgggcag tcgcaattga ccgacaaagc cattaaactg ctggcggcct atcacgagct
     1861 tcatgttaaa atggacgatg ccgtggagaa tgcgtatgaa gaattcaaaa aattcacctc
     1921 tattaagtag tccgacgact gaagccgtcg gttccaattt atataaattc ttcggaacca
     1981 acggctttag tcgttggagc aagatattaa tatcacaact tgctgtgata ttcctgcttt
     2041 cctgcaacaa tcagccgaat ccgccccgtg ataaaagcac aggattgtct ggcgacctca
     2101 tcattttcca cgccggcagc ctgtcgtacc cgttccagaa gattgcccgc gcttttgagg
     2161 cggagaatcc gggtgtgcgc gttttgctgg aagcggccgg cagcgtggat tgtgcccgga
     2221 agatcaccga actgcacaaa ccctgcgacc tgatggcctc ttccgactat ctcgttatcc
     2281 aaaagtttct tatcccgggg cataccggct ggttcctgcc tttcgccggc aacgagatcg
     2341 tgctggcgta cacagataag tcggcttacg gagactccat ctcaagccaa aactggcccg
     2401 aaatcctgat gaaaagcgat gtgcattacg gaaggtccga tcccgatgcc gatccctgcg
     2461 gataccgcac gctgatggtc atgcaactgg caggcgattt ttacggtaac caaggcctta
     2521 ccgagagcct tgcttcaaaa gacaataaat acatccgccc gaaggaggtc gacctgctgg
     2581 ccctgctgga actaaacgag acagattata tcttcattta caggtcggtt gccgtgcagc
     2641 atgggctgaa attcattaag ctgccagatg aaataaacct gaaaataact gagaatgctt
     2701 ccttttacag aaaagcaagt gtggagatca aaggcaccag ccccggcaca aaaatgatga
     2761 tcaacgggga accgatttca tacgcgatta ccatgctgaa tgaatcgccg aatcccggag
     2821 tggcccggca attccttcat tttatgctgg acccggataa ggggatgaaa atcctggaag
     2881 aaagcggcca caactccctc atcccggtcc cttacaaaga cgaattcaat ttacccggtt
     2941 ttctaactaa atatgctctt tatcagaaat gaaaaagatg acgattattt ctcttatgat
     3001 tatttcagca atgatttcag aaccagccag aagccagaca cagaccaatg atttttatga
     3061 tgatgcggag acctataacc ttgccgggat tacgaaaata atgcttgacg gtgagattgg
     3121 taatccggga actgttgaga taaccgcctt tccgctgagg tcgctgatca ttaaagaggc
     3181 cgtgttgtcg ggcgattcaa accgcttcac gggcgcttac cggtacgatg ggtattcgtt
     3241 gtatgatatt ctcaatcatg tcgttctgaa aaagaagaac gaagccgaat ttcccccgat
     3301 catcgatctg tacgtgaaag tcgaaaatgc aaaaggggaa tcggttgtct ttagctgggg
     3361 tgagatctac tatcccgttc accgccatga ggtcattatt gcgacgaaag tcgcacgcat
     3421 tgtgccgtct aagacgaaag acctatggcc gctgcctaca gatattaaaa ttgtggcggg
     3481 ccatgacctg atcaccgaca ggaatatttc caacccgcta aagatcacgg ttctttctgc
     3541 caatcaatcc tatccggtca ataaaggcct gagtccgctc ttttctccgg aaattaaggt
     3601 aatgagcgac ggcaagctat tggatcaaat tgacccatcc agggatttgg gaatgaaact
     3661 gacctacgaa acgatatttt acggcagagg gagagggata cacagcacga tgccctttca
     3721 agggattccg ctgaaagaag ttcttaaaaa gtattttaag attaccgata aaaaccttaa
     3781 gactggttat tttgtggtgg cttctgttga tggttaccgg gttgttttca ccttcagcga
     3841 gatattcaac cggaacgacc aggctgaatt gcttttgata gaagataagg acaaccatga
     3901 cggcggggcg ttccggttgt tcccggcagc cgatttcttt tcagaccggg cagtgaaggc
     3961 ggtttcggaa ataagatttg agatggagtc tcagtaaatg agatggagtc ccttttgagg
     4021 agatggagtc ccattcacgt tt
//
LOCUS       DAOPNH010000293         3982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_26876_length_3982_cov_6.017316, whole genome shotgun sequence.
ACCESSION   DAOPNH010000293 DAOPNH010000000
VERSION     DAOPNH010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26876_length_3982_cov_6.017316"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..718
                     /gene="pdxH"
                     /locus_tag="PKZ74_12780"
     CDS             77..718
                     /gene="pdxH"
                     /locus_tag="PKZ74_12780"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPM93676.1"
                     /translation="MQNHDLSRIRNEYLQGELRTENVLPDPMQQFAVWMDQAILAEIE
                     EPTAMTLATVGPDGAPSARMVLLKGIDEEGFIFFTNYDSRKGQEISRNHRVALVLYWR
                     ELERQVRIEGMVFKASGQESDEYFMSRPAESRVNAIISPQSTVIPGREHLENLRKEYF
                     RTFTGEHKRPPYWGGYHVVPEMIEFWQGRPNRLHDRLRYSRKGGEWIVERLAP"
     gene            759..3020
                     /locus_tag="PKZ74_12785"
     CDS             759..3020
                     /locus_tag="PKZ74_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM93677.1"
                     /translation="MRTGFCAILLLLSSLALGQAGSAGISDKFQGNYDFAWAGGLNAC
                     QFGKIDIDLDGIDDLVVFDRVGDRLLPFVAVTGNNGPDYRYAPEYERNFPDLVHWAIF
                     ADYDRDGKTDIFTYSPPYAGMKVFRNTSGTELEFSLAVFPYLTSFQGGGYVNILVTYA
                     DYPAITDIDDDGDLDILTFWGLGSFVEKHQNMSMEKYGHSDSLDFVKTDYCWGYFAES
                     EESNEIFLDTCLRCLNVIPGIARDRHTGSTFCVTDLNGDQLPDLLLGDVDYPNLVALY
                     NGGNSDTARMTSYDWQYPPSSPVDLFTMPVAFYNDIDFDGIAELLVSPFDPNPFQAEN
                     FQSIWFYDNYGTNDEPVFGLNSTSFIQDRMLDFGAGAYPVFTDINADGLMDIIAGNYG
                     YYDSSYYDQYGLLHTVQTGKLAYLRNIGSASQPAFILEERDFAGISGWKLKGIVPAFG
                     DLDNDGDTDMLTGNETGSLIAYRNNAGAGNIAEFELWENNNPVSDINVGDYSAPQLFD
                     LDNDGKSDLIIGERAGNLNYYRNTGTLNDPVFTYITDSLGKINVTDYSVSLDGFSTPC
                     FFRGNDDITRLVVGSESGELHYFTNIDGNLAGAFEPTSGLSSLLDIQPFDTDRGYRTS
                     AAIIDLDNDGYPEMIAGNFSGGLEYFSRNGQSPVSGIHHESDATFQVNVFPNPSSGKF
                     RIWVESGFPDLTSEVNLFDITGKPLFSSKFVSARDISIQVPDLQPGLYILRIKFMSDP
                     TRFYYHSEKIIVY"
     gene            3147..>3982
                     /locus_tag="PKZ74_12790"
     CDS             3147..>3982
                     /locus_tag="PKZ74_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93678.1"
                     /translation="MKIYIFPVIALLLISLGANAQTVSFENKTAQPTGYTTVVVTASG
                     FPSNVAAVTIYIGVDANVAQCIGSIPGVLPGANVSQASNNRIGIVWTGGGGGYNINGT
                     LCTLIMHYNCGSTVLDGIEGIDTMSIVTPLFEEIPYTYINGSVSAGIYTINTYYVDAA
                     RPSSGDGLSWGTAKKTIQEAANLSLKPGEQVLIKPGTYAETVTIKSNGGYLLRPTTGV
                     VLSDNNKITFPSGAVLPCIDLVNYPGQYYAYIYRSWVSNNGYYKITEVNTGSNFIRVD
                     GAS"
BASE COUNT         1011 a          945 c          952 g         1074 t
ORIGIN      
        1 tccttcgtga aatccttggt gttcctttgt ggtttattac ttttgataca gcctcacttt
       61 tctctacctt tgcctcatgc aaaaccacga cctttccagg ataagaaacg aatatctgca
      121 gggggaactg cggaccgaaa acgttcttcc cgatccgatg cagcaatttg ccgtctggat
      181 ggaccaggct atcctcgcgg agatcgagga gccgacggcc atgaccctgg ctactgttgg
      241 gcctgatggg gcaccatcag cacggatggt ccttcttaaa ggtatcgatg aagaaggatt
      301 tatctttttc acaaactatg atagccgtaa aggtcaggag atttcccgca atcaccgggt
      361 ggctcttgtc ctttactgga gagaactgga gcggcaggtg cggatagaag gaatggtatt
      421 taaagcttcc ggacaggaat cggatgagta ctttatgtcg cgtccggccg aaagccgggt
      481 gaatgcgatc atatccccgc aaagcacagt catacccggg cgtgaacatc tcgagaattt
      541 gcgaaaagag tatttccgga cctttaccgg cgaacataaa cggccgcctt actggggcgg
      601 ctatcatgtc gtccccgaaa tgatcgaatt ctggcaggga agaccgaacc ggctgcacga
      661 ccggctcaga tattcccgga aaggagggga gtggatagta gaacggctgg caccgtgagc
      721 aggaaaaggg aatagggaaa tgggaaatag gataagtatt gagaacaggt ttttgtgcca
      781 tattactttt attatcctcc ctggccctgg gtcaggcagg ttctgccggt atttctgata
      841 aatttcaggg aaattacgat tttgcatggg ccggaggact gaatgcctgc cagttcggta
      901 agatcgatat cgacctcgac ggaatcgacg acctggtggt gttcgaccgc gtgggcgaca
      961 ggcttctccc tttcgttgcc gtaaccggca ataatggccc ggattaccgc tatgccccgg
     1021 aatatgaacg caattttccc gacctggtac actgggccat ctttgccgat tatgaccgtg
     1081 acggaaaaac cgatatcttc acctattccc cgccatacgc cgggatgaag gtcttccgga
     1141 atacctccgg tacggaactg gaattctctc tggcagtttt cccatacctg acctctttcc
     1201 agggaggagg ttatgtaaat atactggtta catatgctga ttacccggcg ataactgata
     1261 ttgacgacga tggcgatctt gatatattga ctttctgggg attagggtca ttcgttgaaa
     1321 agcaccaaaa tatgtcgatg gagaaatacg gccattccga ttccctcgac ttcgtcaaaa
     1381 cggattactg ctggggctac ttcgccgaaa gcgaagaatc caacgaaatc ttcctcgata
     1441 cctgcctccg ctgcctgaac gtcatcccgg gcatagcgag ggacaggcat accggctcca
     1501 ctttttgtgt cacagatctg aatggcgatc aacttcccga cctgttgctc ggcgacgtcg
     1561 attaccccaa cctggtggct ctttataacg gcggtaattc cgatacagcc cggatgactt
     1621 cctacgactg gcagtatccg ccttcttccc cggtagattt gtttactatg ccggtagcat
     1681 tttacaacga tattgatttc gatggcattg ctgaattact ggtctcgcct ttcgatccga
     1741 acccgttcca ggcggaaaat tttcaaagta tctggtttta tgataattat ggcacaaatg
     1801 atgagcctgt ttttggcctt aattcaacct catttatcca ggaccggatg ctcgatttcg
     1861 gagccggcgc ttaccctgtt tttaccgaca tcaatgcgga tggcctgatg gatatcatcg
     1921 ccggcaatta tggctattac gattcatcgt attacgacca gtatggcttg cttcatacag
     1981 ttcaaaccgg caaactggct tatctgagaa atatcggatc ggccagccag ccggcattca
     2041 tcctggaaga gagggatttc gccggtattt ccggttggaa gctcaaaggg attgtccctg
     2101 ctttcggcga tctcgataac gatggtgata cagatatgct gaccggcaat gaaacgggta
     2161 gccttatcgc ttatcgcaat aatgcaggag ccggaaatat tgcagaattt gaattatggg
     2221 aaaacaacaa cccggtttca gacattaatg tcggggatta tagcgcccct cagctgtttg
     2281 acctggataa cgacggcaaa tcagacctca ttatcgggga acgcgccggt aatctgaact
     2341 attaccggaa taccggcacg ttgaatgatc ccgtctttac ttatataacc gacagcctcg
     2401 ggaagatcaa tgttaccgat tattcggttt cgcttgacgg cttcagtacc ccttgttttt
     2461 tcagggggaa tgacgacatc acccgcctgg ttgtcggttc ggaaagtggg gaattgcatt
     2521 actttacgaa catcgatggc aacctggccg gagcgtttga accgacctcc ggcctcagct
     2581 cattacttga tatccagccg tttgataccg atcgtgggta caggacttcc gcagccatca
     2641 tcgatcttga caacgatgga taccctgaga tgatcgcagg gaatttctcc ggcggactag
     2701 aatatttcag ccggaacgga cagtcgccgg taagtggtat tcatcacgaa agtgatgcca
     2761 catttcaggt aaatgttttc ccaaacccat catcgggtaa attcagaatt tgggttgaaa
     2821 gcgggttccc tgatcttact tccgaggtta atcttttcga tatcaccggt aaaccattat
     2881 tctcttccaa atttgtttct gcccgcgata tctcaataca agtacctgat ctacaaccgg
     2941 gattatacat tctcaggatt aaattcatgt ccgatcctac ccgtttttat tatcattctg
     3001 aaaaaatcat cgtttactga aaattgttat ttttttgtta tccggcctta gtgcagcaaa
     3061 ataattagga tttttgaggc gttttattta gaattgttat aaatttgaaa tttgaagttt
     3121 aaaaatttgc tgttgttata aaaatcatga agatttatat atttcctgta attgccttat
     3181 tattaatatc tttaggggct aacgctcaaa ctgtttcgtt cgagaacaag acagctcaac
     3241 ccaccggata cacaaccgtg gtcgttacag cttcaggatt tccttctaat gtcgcagcag
     3301 ttactattta tatcggagtt gacgctaatg tggcccaatg catcgggagc atcccgggag
     3361 tccttccggg tgcaaatgtc tcccaggctt caaacaacag gatcggtatt gtctggacag
     3421 gcggcggcgg cggatacaat ataaatggta cgctatgcac ccttattatg cactacaact
     3481 gcggttcaac cgtcctcgac gggattgaag gaatcgatac catgtcgatc gttacgccgt
     3541 tgtttgaaga aattccatat acctacataa acggctctgt ttcagccggc atttatacca
     3601 taaacaccta ttatgtcgat gccgctcgtc cttccagcgg tgatggatta agctggggaa
     3661 cagcaaagaa aacgatacag gaagcagcaa acctgtcttt aaagcccggt gagcaggtgc
     3721 tgatcaaacc cggtacttat gctgaaaccg taacgattaa atcaaatggc ggctacctgc
     3781 tcaggccgac caccggtgtt gtgctttcgg ataacaataa aatcacattt cccagcggtg
     3841 cggtattgcc ctgcatcgac ctggttaatt atcccgggca atattatgcc tatatatacc
     3901 ggagctgggt gtcgaataac ggatactata aaataacgga agtcaacact ggttccaatt
     3961 ttatcagggt cgacggggct tc
//
LOCUS       DAOPNH010000294         3967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27015_length_3967_cov_4.025818, whole genome shotgun sequence.
ACCESSION   DAOPNH010000294 DAOPNH010000000
VERSION     DAOPNH010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27015_length_3967_cov_4.025818"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1229)
                     /locus_tag="PKZ74_12795"
     CDS             complement(120..1229)
                     /locus_tag="PKZ74_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPM93679.1"
                     /translation="MFAVKAICLTFILATTGYLSSGQIPEGYYSTASGLSGSALKAAL
                     HDIIDDHTELSYTATVNALKVTDEDPNNSSNVILIYTGWSYPKSSYSYDWNREHVWAK
                     SHGDFGDYPPEGTDLHHLRPCDADVNSTKSNRDFSKGTSPYFLNGIPTGCYTAPYIWE
                     PRDEDKGDVARMMFYMAVRYEGDNGETNLELVSYVNSSPDNEPLYGNLDTLFKWHNND
                     PVSAWEISRNNKIYSQYQGNRNPFIDHPEYVDLIWGDGIADEGEPVSHASDFTASNII
                     LNWNEPTTGVLPDAYLIGISTSSFEAIPVPADGVPVENSSISKNIAYGIGQYTFKNAI
                     PGTTYYFKIYPYKGFGEAINYKTDGNIQQVMIEIN"
     gene            complement(1399..1857)
                     /locus_tag="PKZ74_12800"
     CDS             complement(1399..1857)
                     /locus_tag="PKZ74_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93680.1"
                     /translation="MKAFKSDILPKQGFGEIMFGETSEKVIKLLGQPEDVENIEDVDG
                     FNTVVLYYYEKGITIFFEGKEKSVVACVETENPEATMYGKRIFEMTEKDIVALMKEKG
                     FEVAETEMETTGERRVSYDDAMIDFFFLDGDLVVVNWGVLVNEKGEIEEI"
     gene            complement(1863..2669)
                     /locus_tag="PKZ74_12805"
     CDS             complement(1863..2669)
                     /locus_tag="PKZ74_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM93681.1"
                     /translation="MKDKVIIVTGASSGIGKALAAELSRRGAIVVLAARSADKLSELE
                     KELGGKGSKVLGVVTDVSKEEDCRKLVSKTVEKFGKIDVLINNAGISMRAIFEEVELD
                     VIRRLMDINFWGTVYCTKYALPYLLKTKGSVVGVSSIAGFKGLPGRTGYSSSKFAMQG
                     FLETLRIENLKKGLHVMIACPGFTSSNIRNTALAADGSMQGESPRDEAGMMSPEEVAR
                     RIAKGIENRRKLLVMTSQGKMTVILNKFFPYFIDKMVFNHMAKEPDSPFK"
     assembly_gap    2794..2893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3032..3105
                     /locus_tag="PKZ74_12810"
     tRNA            3032..3105
                     /locus_tag="PKZ74_12810"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3066..3068,aa:Met,seq:cat)
     gene            complement(3196..>3967)
                     /locus_tag="PKZ74_12815"
     CDS             complement(3196..>3967)
                     /locus_tag="PKZ74_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPM93682.1"
                     /translation="KTNYLHTYNNIMSSLRSFFNHLIKYEEFDIRNIFTLVPVHSVHY
                     DPVTFTAEEFDKILEVTTIENGYDKTKKRNHYRSWLKTSFKLGAFTDLRLDELVHMKY
                     SDIEMKDGVLVLQAENMKATKLIGAKQPKDKRIKRIPVIEELRKVLYEECEYEQNIGS
                     DKFIIAPELARITVHNTICKGFTHFKRLAGIDEQKCFKELRKTYMNQSEAQFGITLTT
                     LVSDHSNDEVVRKHYLAQMEAVKKTAGLKIFPKKDENE"
BASE COUNT         1004 a          857 c          804 g         1202 t
ORIGIN      
        1 caaaatttat gatatggtca gtaccggtac tggtaatcga aagttgacca gatgcagttt
       61 cggttagtat aacaattgca gataatacta aaatgggtaa tagggttatc tttctcatat
      121 taattaattt ctatcattac ttgttgaatg tttccgtcgg ttttataatt gatggcttct
      181 ccgaagcctt tgtaaggata tatcttaaaa taataagtcg ttcccggaat ggcattttta
      241 aaggtgtact gaccgattcc atatgcaata tttttactga tggaactgtt ctccaccggg
      301 acgccatccg ccggaacagg aatagcctca aaactgctgg tgctgatgcc gatcaggtaa
      361 gcatcgggca atacccccgt cgtgggctcg ttccagttca gaatgatgtt ggaggccgtg
      421 aaatcagagg catgcgatac aggctcccct tcatcggcaa tgccatcacc ccatatcaga
      481 tcaacatatt ccggatgatc gatgaatgga ttacggttgc cctggtattg gctgtagatc
      541 ttgttgttac gggatatctc ccaggcgctc accgggtcgt tgttgtgcca tttaaacaac
      601 gtatccaggt tcccgtacaa tggttcgttg tcaggggagg agttgacata actgaccaat
      661 tccaggttgg tttccccgtt gtcgccttcg taccttaccg ccatataaaa catcatccgg
      721 gccacatcac ccttgtcctc atctctcggt tcccagatat aaggagcggt atagcatcct
      781 gtaggaatac catttaaaaa ataaggtgag gtcccttttg aaaaatcacg gttgctcttt
      841 gtggaattga cgtctgcatc gcagggcctg agatgatgca ggtcggttcc ttccggagga
      901 tagtccccga aatcgccatg cgatttagcc cacacatgtt cccggttcca atcataggaa
      961 taagaagatt tcggatacga ccatccggta taaattagta tgacattgct gctattgttc
     1021 ggatcctcat ccgtcacttt taatgcatta acggttgccg tataactcaa ttcggtgtga
     1081 tcgtcaataa tatcatgcag tgcggctttt aatgcactcc cgctcagccc tgatgcggtc
     1141 gagtaatatc cttcgggaat ttgacccgaa gataaatacc cggtagtggc cagaatgaag
     1201 gtcaggcata tggcttttac tgcaaacatc agtaaatcat ggtgttataa agcggctaaa
     1261 atgtcctgaa ttttcaggag tgcaaagtta ctaacagttg caggacattt ttgtgatgtt
     1321 gataaatata aagaaaagtt aatgtttgaa acgatatgga cctattaata aaacgaatgt
     1381 attgttacaa acgatatatt aaatctcttc gatttcccct ttttcgttaa cgagaactcc
     1441 ccagtttacg acaaccaggt cgccgtcgag gaagaaaaag tcgatcatgg catcgtcata
     1501 cgataccctg cgttcacccg tcgtttccat ctctgtttcg gccacctcga aacctttttc
     1561 tttcatcagc gcgacaatgt ctttttcggt catttcaaag atgcgcttgc catacattgt
     1621 ggcttccggg ttctcggttt cgacgcaggc caccaccgac ttttccttgc cttcgaagaa
     1681 aattgtgata cctttttcat aataataaag gaccacagta ttgaacccgt cgacatcttc
     1741 gatattttcg acatcctcgg gctgaccgag cagtttgatg accttttccg atgtctctcc
     1801 gaacatgatt tccccgaagc cctgtttggg gagaatgtct gatttaaatg ctttcatttt
     1861 atttatttaa aaggtgaatc cggttctttg gccatatggt taaagaccat tttatcgata
     1921 aagtaaggga aaaatttgtt aagaatgacg gtcattttac cttgagaggt catgaccagc
     1981 aactttctcc tgttttcaat tcctttggcg atccttcggg caacttcctc gggcgacatc
     2041 ataccggctt catcccgggg tgattccccc tgcatcgagc catcggcggc taaagcggtg
     2101 ttcctgatat tggaagaggt aaatccaggg caggcgatca taacatgcag tcccttcttc
     2161 aggttctcaa tgcggagcgt ctccagaaat ccctgcatgg caaattttga tgaggaataa
     2221 ccggttctgc ccggcaaacc cttaaaaccg gctatggaag aaacacccac gacggatcct
     2281 tttgttttca aaagataggg gagggcatat tttgtgcagt aaaccgttcc ccagaagttg
     2341 atgtccatca gcctgcggat tacatccagt tcaacctctt caaagattgc ccgcatggag
     2401 atcccggcat tattgatcaa aacatcgatc tttccgaatt tttccaccgt tttgctgacc
     2461 aacttccggc aatcctcctc tttgctcaca tcggtaacaa caccaagtac cttgctgcct
     2521 tttccaccaa gttctttttc cagctccgac agcttatcgg cagagcgcgc tgccagtaca
     2581 acgatcgctc ctcggcggct tagctctgca gccagggctt tcccgatacc cgatgaagct
     2641 cccgtaacga ttataacttt atctttcatt acatacaatg ttaggatatt tttcaatagt
     2701 tggaagatat tttttgttaa gaatttaaaa aatcctggca tatgagaatt aaagataaaa
     2761 aagtgctaat tttgcccgct caaaaatggc gagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnncatatga gaattaaaga taaaaaagtg ctaattttgc ccgctcaaaa
     2941 atggcgaggt agctcaggtg gttagagcgt cggattcata tccgccggct ggcggacacg
     3001 agttcaattc tcgcaaagtt atttgtttaa tggcgaggta gctcaggtgg ttagagcgtc
     3061 ggattcataa cccggaggtc gagggttcaa ttccctctct cgctacattg aaagtcaggt
     3121 atttacctga cttttttatt ttctgtctat ttatgaggta aggtgacgag gtaaggaaaa
     3181 tttttgaaag tttctttatt cattctcatc tttctttggg aagatcttta gcccagccgt
     3241 ctttttaacg gcttccatct gggcgagata atgtttcctt acgacttcat cattactatg
     3301 atcgcttaca agcgtggtga gtgtaatacc aaattgagct tcagattggt tcatataggt
     3361 ctttcgtaat tctttaaaac acttttgttc atcaattcca gccagtcgtt taaaatgagt
     3421 gaaccctttg catattgtgt tatgaaccgt tattcttgct aattctgggg caattatgaa
     3481 cttatccgac ccaatgttct gttcatactc acattcttca taaagaacct ttctcaattc
     3541 ttcgataacg gggattcttt taatacgttt atcttttggc tgtttcgccc caatcaattt
     3601 ggttgctttc atattttctg cttgtagaac caatacgcca tctttcatct ctatgtcgga
     3661 atatttcata tgcactagct cgtccagccg taaatcggtg aatgctccca gtttgaatga
     3721 tgttttcaac cagcttcggt aatgatttcg ttttttggtt ttatcatacc cattctcgat
     3781 ggtggttact tccaatattt tgtcaaattc ttccgcagta aatgtcacag ggtcatagtg
     3841 gacagaatgg actggcacta gggtaaatat gttcctgatg tcaaactctt catatttaat
     3901 caaatggttg aaaaagctac gcagagaact cataatatta ttataggtat gcagataatt
     3961 ggttttc
//
LOCUS       DAOPNH010000295         3964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27043_length_3964_cov_5.632387, whole genome shotgun sequence.
ACCESSION   DAOPNH010000295 DAOPNH010000000
VERSION     DAOPNH010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27043_length_3964_cov_5.632387"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PKZ74_12820"
     CDS             <1..983
                     /locus_tag="PKZ74_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM93683.1"
                     /translation="EFANPGFLYLLLLVPMLIAWYIFRHGRSIPTLQVSDTAVFENNR
                     RSFRHYLSHLLFVLRMVAVALLIIAFARPQSTTKRQNVSIEGIDIVLGLDISGSMLAQ
                     DLKPDRLEAAKKVSLDFFQGRPNDRIGLVVFAGEAFTQCPLTTDHVIIEDMLDDIESG
                     IIQDGTAIGDGLATAINRLKESQAVSKVIILLTDGQNNAGSIAPLSAAEIAKIYGIRI
                     YTIGVGTIGFAPYPVQTPFGVQYQNMEVKIDEDLLQKVANMTDGKYFRATDNTKLREV
                     YQEIDQLEKSKIDVTEFRKKHEEYLILAVWALILLALESLMRFTLFRSLP"
     gene            983..2020
                     /locus_tag="PKZ74_12825"
     CDS             983..2020
                     /locus_tag="PKZ74_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM93684.1"
                     /translation="MYRFEHIEYLYALLVIPVLTVLFILYLIWKKKAVKKFGDQAVVS
                     LLMPMASARKLTTKFVVLMLAFATLVIGLANPQIGSKLEKVQRKGADLMIALDVSNSM
                     LSQDIRPDRLTRAVQGISKLIDRLEGDRIGIVVFAGKAYVQLPITTDYSAAKMFLSTI
                     STQMVPSQGTAIGEAIDLSLKSFKTDERSKAIIIITDGENHEGNAVEAAQAAAENGIK
                     IFTIGMGSPEGSPIPLFDKFGRQTGYKADRDGRTIISRLDETTLQQVASAGNGIYVRA
                     STGQDGLSKILDEINALEKQEIETRMFSEYEGRFQYFLAIALFFLILEFFIPDKKSLW
                     TDKIKLFNRLS"
     gene            2023..2790
                     /locus_tag="PKZ74_12830"
     CDS             2023..2790
                     /locus_tag="PKZ74_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93685.1"
                     /translation="MKRHLHILLLLLLPAPLLVAQQENRFIRQGNKQYDDEKFKEAEV
                     SYIKSTQAKNASHKGFYNLGNAWYMQENYLQASAAFDTLRTFRMDDDTRAKSYYNLGN
                     AMLKSAQDSAQLAGKFLPASVEAYKQSLRLNPEDEEAKYNLAYAQKLMQQQQQQQQQQ
                     QQNQDQKDQQKEQQKDQQKQQDQKDQQQDQQQQDQQNAEQANQDQQQANAEPQQISKE
                     DAERILEALKNDEKETLEKLSEAKIQSVRVVKTDKDW"
     gene            2807..>3964
                     /locus_tag="PKZ74_12835"
     CDS             2807..>3964
                     /locus_tag="PKZ74_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPM93686.1"
                     /translation="MNRILKIMFVLALMIPVFGILNGQDIEFSAATKNNVQVGEQFRA
                     VYTVNSKVDRFNGPDFEGFNVLSGPNSSQNQSYQFINGKVSQSFQITYTYYLQATREG
                     TFTIPAASISIGNQTFQSNPLTINVSQSASGQAPPAQGNVRQANRNTGTPGNNEIFIK
                     ASVDKAKPFQGEQVIITYKIYTAVPVSQINITKISSFPGFWSKNLLGDDEQLKQTTEY
                     INGKEYVVAELRKLALFAQRSGELKIEPMEMECLAQVKVESQRTRDPFFDSFFNDPFF
                     SRNYQNVEIKLESNPLTIDVTPLPASGKPLDFSGAVGSFTLSSEIDRNELKTNEALNL
                     KVTITGKGNLELIEALPLTFPPDFEVYDPRITNNLSKSTTGVSGSRVFEILG"
BASE COUNT         1096 a         1025 c         1006 g          837 t
ORIGIN      
        1 tagaatttgc caatcccgga tttctgtacc tgctgcttct ggtaccgatg ctgatcgcat
       61 ggtacatttt caggcatggc agaagcattc cgaccctgca ggtttcggat acggcggtat
      121 ttgaaaacaa ccggagaagc ttcaggcact atctttctca tctcctgttt gtgttgcgca
      181 tggtggccgt tgccttgctt atcattgcct ttgcccggcc ccaatcgact accaagcggc
      241 agaatgtcag tatcgaaggc attgatatcg tgctgggact ggacatttcg ggcagtatgc
      301 tggcgcagga tctgaaaccc gaccggctgg aagcggccaa aaaggtcagc ctcgatttct
      361 tccagggccg cccgaacgac cggatcggcc tggtggtttt tgccggagaa gcctttacgc
      421 aatgcccgct gaccaccgac catgtgatca ttgaagatat gctggacgac atcgaaagcg
      481 gtatcatcca ggacggaacg gccattggcg acggactcgc caccgccatc aaccggttga
      541 aagagagcca ggctgtcagc aaggtgataa tcctgctgac cgacggccag aacaatgccg
      601 gtagcattgc gcccctctcg gccgccgaaa ttgctaaaat atatggtatc cggatctata
      661 ccatcggcgt cggaaccatc ggttttgccc cttaccccgt gcagaccccc ttcggcgtgc
      721 aataccagaa catggaggtc aagatcgatg aggatctcct gcaaaaagtc gccaatatga
      781 ccgacgggaa atatttccgt gctaccgata acaccaaact gcgcgaagtt taccaggaaa
      841 ttgaccagct tgaaaagtcc aagatcgatg tgaccgaatt caggaaaaaa cacgaggaat
      901 acctcatcct ggcggtctgg gccttgatat tgctggcact ggagtcgttg atgagattta
      961 ctttattcag aagtttaccc tgatgtaccg attcgagcac atagaatacc tttatgccct
     1021 gctggtgatc ccggttctga ccgtcctttt cattctttac ctgatatgga aaaagaaagc
     1081 tgtgaagaaa ttcggcgatc aggctgtagt ttcgctcctg atgccgatgg cctcagccag
     1141 gaagctgacg accaaattcg ttgttttgat gctggctttc gccacgctgg tgatcgggct
     1201 ggccaacccg cagatcgggt caaagctgga aaaagtccag cgtaagggcg ccgacctgat
     1261 gatcgccctc gacgtgtcga acagcatgct ctcgcaggat atccgccctg accgattaac
     1321 ccgggccgtg cagggaatct ccaagctgat cgaccggctg gaaggcgacc ggatagggat
     1381 cgtcgttttc gccgggaaag cctatgtcca gcttcccatc accaccgatt attcggctgc
     1441 aaaaatgttc ctgtcgacca tatccacgca aatggtaccc tcacagggca ctgccatcgg
     1501 cgaagccatc gacctctccc tgaaatcctt caaaacggat gaaaggagca aagcgatcat
     1561 catcatcacc gacggggaga atcacgaggg aaatgcagtg gaggccgcgc aggcagcggc
     1621 tgagaacggg atcaagatct tcaccatcgg gatgggatca cccgaagggt cgcctatacc
     1681 cttgttcgac aaattcggca ggcaaacggg ctacaaagcc gaccgggatg gccgcacgat
     1741 catctcccgg cttgatgaaa caaccctgca gcaggtagcc agcgccggga acgggatcta
     1801 tgtcagggcc tccaccgggc aggacgggct ttccaaaatc ctggacgaaa ttaatgccct
     1861 tgaaaaacag gaaattgaaa cccggatgtt ctcggaatat gaagggagat tccagtattt
     1921 tctggccatt gccttattct ttttgatcct ggagtttttt attccggata aaaaaagctt
     1981 atggacagac aaaatcaaac tgtttaaccg tttatcataa ctatgaaacg ccatctccat
     2041 atccttctcc tgttgctgct gccggcgccg ttactggtgg cccagcagga aaaccgcttt
     2101 atccggcagg ggaacaagca atatgatgat gaaaagttta aagaagccga ggtcagctac
     2161 ataaaatcca cccaggcaaa gaacgcctcg cacaaaggat tttacaatct cggaaatgcc
     2221 tggtatatgc aggaaaatta cctccaggca tccgctgcat tcgataccct gcggaccttc
     2281 agaatggatg atgatacccg ggcaaaatca tattataacc tgggaaatgc catgctcaag
     2341 agcgcccagg acagtgcaca gctggccgga aaattcctgc cagccagcgt tgaagcttac
     2401 aaacagtccc ttcgtctcaa cccggaagac gaagaagcca aatataacct ggcttatgcc
     2461 cagaaactga tgcagcaaca gcagcaacag cagcagcaac aacagcaaaa ccaggatcag
     2521 aaagatcagc aaaaggagca gcagaaagat cagcagaaac agcaggatca gaaagatcag
     2581 caacaagatc agcagcagca ggatcagcag aatgcggaac aggccaacca ggatcagcag
     2641 caagccaacg cagagcccca gcagatttca aaggaggatg ccgaaaggat cctggaagcg
     2701 ctgaaaaatg atgaaaaaga gacgctagag aagttgtcgg aagctaaaat tcaatcggtc
     2761 agggtggtaa agaccgataa agactggtag gcaatacgga attattatga atagaatcct
     2821 gaaaataatg tttgttctcg ccctgatgat cccggtcttc ggcattctca acggccagga
     2881 cattgagttc tcggcggcca cgaagaacaa tgtccaggtc ggggaacaat tcagggctgt
     2941 atatacggtg aacagcaagg ttgaccggtt taacggcccc gattttgaag gatttaatgt
     3001 actgagcggg ccgaattcct cgcaaaacca gagctaccag ttcatcaacg gcaaggtgtc
     3061 gcagtcgttc cagatcactt acacttatta cctgcaggct acccgggagg ggacattcac
     3121 gatcccggcg gcctcaattt cgatcggcaa ccagaccttc cagtccaatc cgctgaccat
     3181 caatgtcagc cagtcggctt caggacaagc acctccggca cagggcaatg taaggcaggc
     3241 aaaccggaac acaggcacac ccggaaacaa tgagattttc atcaaagcca gtgtcgataa
     3301 agcaaaaccg ttccagggag aacaggttat catcacctac aaaatttaca cggctgtgcc
     3361 cgtttcacag atcaatatca cgaaaatctc ctctttcccg ggtttctggt caaaaaatct
     3421 gttgggtgat gacgaacagt taaaacagac gactgagtat atcaacggga aggagtatgt
     3481 cgtggccgaa ttgcggaaac tggcgctttt tgcccagcgc agcggcgagt tgaagatcga
     3541 gccaatggag atggagtgcc tggcccaggt caaagtggag agccaacgga cacgggatcc
     3601 cttcttcgac agttttttca atgatccttt tttcagcagg aattaccaga acgtggaaat
     3661 aaagcttgaa tccaatccgc tgacgattga tgtaaccccg ctgccggctt ccggtaagcc
     3721 cctggatttc agcggagcgg tgggatcgtt caccctttcc tcggagattg accgtaacga
     3781 actgaaaacc aatgaagcgc tgaatctgaa agtgaccatt acgggcaaag gcaacctcga
     3841 actgatcgaa gcgcttccat tgacttttcc tcctgatttc gaggtctatg atcccagaat
     3901 caccaataat ctcagcaaaa gtaccacagg ggtttcgggc agcagggtat tcgagatcct
     3961 ggga
//
LOCUS       DAOPNH010000296         3945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27231_length_3945_cov_5.601542, whole genome shotgun sequence.
ACCESSION   DAOPNH010000296 DAOPNH010000000
VERSION     DAOPNH010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27231_length_3945_cov_5.601542"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKZ74_12840"
     CDS             <1..442
                     /locus_tag="PKZ74_12840"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPM93687.1"
                     /translation="NLLKKSIIFFIITYLLVTVITFFFLPHLTDNLLENAILLVVPVL
                     AFLSIANIPRLVSKGRYVGAFLFSALTMSFLLILVAVELYPVMVLSTVDPAYSITVYN
                     AASSEKSLGIMLLLTAIGFPLVITYTVFVYKTFWGKVKLDETSY"
     gene            594..974
                     /locus_tag="PKZ74_12845"
     CDS             594..974
                     /locus_tag="PKZ74_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93688.1"
                     /translation="MFSLIPFQAKANVYQKDALDDSPGTGNFALFLRHYQPGFKWVAR
                     PVQPFNPQEHEKKLPEPSAEQFLDKSKRKGISSQGPEPGAPVFTGIFSGYPDHVNNFD
                     PHADLGIDFQYCGIHYRVRPPPAG"
     gene            complement(1179..2621)
                     /locus_tag="PKZ74_12850"
     CDS             complement(1179..2621)
                     /locus_tag="PKZ74_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HPM93689.1"
                     /translation="MKWRSLFIVLAFAAFGVTAQSFNKKEPAKLKTSIIITAQNTNYR
                     LSHQMQEQFKPTPQPWESIPCVFVDTAKLFQTFVGIGGALTDASAETFAKLPAKKQEE
                     FLKAYFDREEGIGYSIIRTNMNSCDFSSGSYTYVDEGDISLKSFNIAHDEQYKLPLIK
                     KAMETAGGKLPLFISPWSPPAWMKGNKNMLQGGTLLREYHPAWAEYCVKFIKAYEERG
                     VPVWGLSVQNEPMAKQTWESCIYTAEEERDFIKNFLGPALWNSGLKDKKLIAWDHNRD
                     LVYHRASTILSDPEAAKYVWGIGFHWYESWTGAPTPENVKRVAEAFPNTNLLLTEACN
                     YPFSWDTFDQWNWGENYGAAMISDFNNGAVAWTDWNVLLDETGGPNHVKNFCFAPVHG
                     DTRDGSLHYMNSYYYIGHFSKFIEPGAKRMAVSSNRAQLQTTGFRNPDGKHVVIVMNQ
                     ADTEVKFRLYVGEQAAEMLSLPHSIMTFVF"
     gene            complement(2642..>3945)
                     /locus_tag="PKZ74_12855"
     CDS             complement(2642..>3945)
                     /locus_tag="PKZ74_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPM93690.1"
                     /translation="LFQRQTDLLVMDSLITNHPVVRIDTSATMQEIEGFGAALTGSSA
                     YLLHQELDPAARTATLKDLFDPAAGIGISYLRLTMGASDFSLSDYTYNDLPAGQTDFE
                     LQQFSLAQDLDDVVPVIKEIIEIAPDIGLMGSPWSPPAWMKTNGSLKGGKLKPECYEV
                     YASYFVRYIQAMAGQGIIIDAVTPQNEPLHFTAGYPCMEMQPEEQGDFIKNHLGPAFQ
                     QAGISTKIIIYDHNWDDTQYPISILNDAAAAQYIAGSAFHAYAGDVSAMSTVHNVHPG
                     KGLYFTEISGGEWAVNFSDNLMWYMDNILIGTTRNWSKTALFWNLALNQANGPQNNGC
                     ADCRGVITIYSNNGSVVKNEEYYALAHFSKFVRPGAVRVSLTIPQLLANMGIVSFQNP
                     DGSKVLVACNYSAADRTFSAQQETRHFTATVPAKSVVTIIW"
BASE COUNT          911 a          968 c          962 g         1104 t
ORIGIN      
        1 caacctgctg aaaaagagca tcatcttctt cattatcacc tatttgctgg tcaccgtcat
       61 tacctttttc tttctccccc atctgaccga taacctcctg gagaatgcca ttttgctggt
      121 ggtgccggtt ctggccttcc tgagcatagc caacattccc aggctggtca gtaaaggccg
      181 gtatgtcggc gccttcctgt tttccgcact gaccatgagt ttcctgctca tcctggttgc
      241 cgttgagctt tacccggtta tggttctttc aaccgttgat ccagcataca gcattaccgt
      301 ttacaatgcc gcctcgtcgg aaaaatcgct ggggatcatg ttgctgttga ctgcaattgg
      361 atttccattg gtcataacct acactgtatt tgtttataag acattctggg ggaaagttaa
      421 gctggatgag accagttatt aatacgaacc gggaggcaat gattggcatt tattgcctat
      481 ttttacgcct gattttccgg ataagagaaa cgatgtttga aatgatcagg gataagaaaa
      541 tattacacat catcggcagg tacacacgta tcccgatttt gtgttgttta ttcatgttct
      601 ccctgattcc ttttcaggcc aaagcgaatg tttaccaaaa agatgccctt gatgattcgc
      661 ccggtacagg gaatttcgcc ttatttctga ggcattatca gcctggtttc aaatgggttg
      721 cccggccggt tcagcctttt aacccgcaag agcatgaaaa aaagcttcct gaaccatctg
      781 ccgaacagtt cctcgacaag tcaaaaagaa aagggatcag ttcgcagggg cccgaacccg
      841 gagcgcctgt gtttaccgga atcttttcag gttatcctga tcatgtcaat aattttgatc
      901 ctcatgctga tttaggtatt gatttccaat attgcggaat tcattaccgg gtgcggcctc
      961 cgcccgctgg ttaggaaaac cgggccgact ttcggttttc cattttcctt cacattctat
     1021 ttatcataca gcatcacctc tgcggagctg tattattatt tcaacaattt aattttatca
     1081 agatggaaga aaagaaaaaa tccagcaatt gggtcgtatt cgccttttta atcggcgtgg
     1141 tagtattgat gatcctggta aaggttttgt ttttctgatc agaaaacaaa agtcatgata
     1201 gaatgaggca ggctgagcat ttccgcagcc tgctctccta catagagcct gaatttcact
     1261 tcggtatcgg cctggttcat aacgatcacc acatgttttc cgtccggatt cctgaaccct
     1321 gtggtttgca gttgggcccg gttggatgag acggccatcc gtttcgcccc gggttcgatg
     1381 aatttggaga agtggccgat gtaataatag gaattcatat agtggaggct gccgtcgcgt
     1441 gtatcgccat ggaccggtgc aaagcagaag ttttttacat gattggggcc tcccgtttca
     1501 tcgaggagca cattccagtc ggtccaggcc acagcgccgt tgttaaaatc ggagatcata
     1561 gccgcgccgt agttttcacc ccaattccac tggtcgaaag tgtcccagct aaaagggtaa
     1621 ttacaggctt ctgtcagcag gagattggta ttcgggaaag cctcggccac ccgtttcacg
     1681 ttttcaggcg tcggtgctcc ggtccagctt tcataccagt gaaaaccgat gccccacaca
     1741 tatttggcgg cttccggatc gctcaggatg gtactggccc ggtgatagac caggtcgcgg
     1801 ttatggtccc aggcgatgag ttttttatcc ttcagtccgc tgttccacaa tgcaggtccg
     1861 aggaaattct tgatgaaatc acgttcttct tcagccgtat atatgcatga ttcccaggtt
     1921 tgcttcgcca tcggttcgtt ctgtacgcta agcccccaca cgggtactcc cctctcttcg
     1981 tatgctttga tgaatttcac acaatattcg gcccatgccg ggtggtattc cctcaaaagt
     2041 gttcctccct gcagcatatt tttatttcct ttcatccatg ccggcgggct ccaggggctg
     2101 atgaacaggg gaagttttcc tcctgccgtt tccatagctt ttttgatcaa cgggagctta
     2161 tactgttcat catgagctat attaaacgat ttcaggctga tatcgccttc atccacgtaa
     2221 gtgtaactgc cgctcgaaaa atcacagctg ttcatattgg ttcttatgat ggaataacca
     2281 atcccctctt ccctgtcgaa ataagccttc aggaactcct cctgcttttt ggccggcagt
     2341 ttggcgaacg tctcagcgga tgcatcggtc aacgccccgc cgattcccac gaatgtctgg
     2401 aacagtttcg cggtatcgac aaaaacacag gggatcgatt cccatggctg gggcgtaggt
     2461 ttgaactgtt cctgcatctg gtgtgataaa cggtaatttg tgttctgcgc ggtgataatg
     2521 atggatgtct tcaattttgc gggttctttt ttattgaaac tctgtgctgt cactccaaaa
     2581 gcagcgaaag cgaggactat gaataacgat ctccatttca tctttttaaa attttgaatg
     2641 attaccaaat gattgttaca acagatttcg cggggacggt tgccgtaaaa tgtcttgttt
     2701 cctgctgtgc gctgaaggta cggtcggcag ctgaataatt gcaagccacg agaacttttg
     2761 aaccgtcggg attttgaaat gaaacgatgc ccatattggc gagcaactgc ggaattgtta
     2821 aagaaacccg caccgcgccg ggccggacaa attttgaaaa gtgggccagg gcatagtact
     2881 cttcattctt caccaccgat ccgttattgg aatagatagt gatcacaccg cggcagtcgg
     2941 cacagccgtt attttgcggg ccgttggcct ggttcagggc aaggttccag aacagggccg
     3001 ttttcgacca attccgggtc gtcccgatta aaatattatc catataccac atcaggttat
     3061 cactgaagtt aaccgcccat tctcctccgg aaatttcggt aaagtagagg ccttttcccg
     3121 ggtggacatt atggaccgtc gacatggccg aaacatcgcc ggcatatgca tggaaagcgg
     3181 agccggcaat atattgcgcg gctgccgcat cgttcagaat cgatatggga tactgtgtat
     3241 catcccagtt atgatcatag ataatgatct ttgtgctgat accggcttgc tgaaatgcag
     3301 gtcccaggtg gtttttgatg aaatcgccct gttcctcagg ctgcatctcc atgcaagggt
     3361 acccggcggt gaagtggagt ggttcattct gaggcgtcac cgcatcgata atgattccct
     3421 gcccggccat agcctggatg taacggacaa aatatgaggc atacacttca taacattcgg
     3481 gtttcagctt accgcctttc aggctgccgt tggttttcat ccatgccggc gggctccatg
     3541 gcgagcccat cagtccgata tcgggcgcta tctcaatgat ttccttaata accgggacca
     3601 catcgtccag gtcctgggcc aggctgaatt gctgaagttc aaaatcggtc tggcctgccg
     3661 gaaggtcgtt ataggtatag tcggataatg aaaagtcaga tgcccccatg gtcagcctca
     3721 ggtaagagat tccgatcccc gctgccgggt cgaacaggtc tttcagggtg gcagtccggg
     3781 cggcgggatc gagctcctgg tgcagcagat aggctgatga tccggtaagg gcggcgccaa
     3841 aaccttcgat ctcctgcatt gtcgcggaag tatcgatccg gacgaccggg tggttggtga
     3901 tcaatgaatc catcaccagc aggtctgtct gacgctggaa caacg
//
LOCUS       DAOPNH010000297         3936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27319_length_3936_cov_5.130894, whole genome shotgun sequence.
ACCESSION   DAOPNH010000297 DAOPNH010000000
VERSION     DAOPNH010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27319_length_3936_cov_5.130894"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PKZ74_12860"
     CDS             complement(<1..1537)
                     /locus_tag="PKZ74_12860"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93691.1"
                     /translation="MRYLFTLSVFCILFIFQSRAQNVSPGISDYSSYPYWIEMMSDAS
                     VNFYDVQEAFNTYYEGKDLTKLKGWKPFKRWEYSMLQGRIFPDGTRRPEDHIVKAYES
                     YLATHPGARSQAGDWTNLGPFVLPDGKGYKGLGRLNAIAFDPEDPDIVWVGSPSGGLW
                     KTTTHGGEWTSSTQELPSLGVSAILIDPTNPDVMYIGTGDRDAGDAYGIGVYRSTDGG
                     DTWEPWNLGMTNYVVGRLLMHPSNHMIIFAATSGGLFRTTNGGATWTRVQTGDFKDVV
                     FKPNNPDIVYAAANGNFYRSSNNGLNWTQITSGLPGSSRSAIAVTPDNPNYVYVILTY
                     GDSFMGLYRSTDAGLNFTMMSNSPNIMSWGCNGGDGGQAWYDLDIACDPNDADVIFAG
                     GVNCFKSVTGGSIWSISSHWWGDCGVPSVHADLHVLEYNPLNDRLYAGNDGGVYYTDD
                     QGETWTEITNGLPIGQVYKIGQSATMRDKTVNGYQDNGTSTYMGNYWQFILGGDGMEC
                     AVDP"
     gene            complement(1644..2501)
                     /gene="cyoE"
                     /locus_tag="PKZ74_12865"
     CDS             complement(1644..2501)
                     /gene="cyoE"
                     /locus_tag="PKZ74_12865"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPM93692.1"
                     /translation="MPLINSYLELGKVKITFAVALTTITGYLLGGGSFDSGLILPTLG
                     IFLLACGSSAINHYQERERDALMERTRHRPIPSGRISPGAALAFAFLMSMAGSGLLYI
                     GSGILAMQLGILALIWYNVIYTPLKKKSAFAVVPGAVIGAIPPLVGWVAAGGSLTDPR
                     AMFMAFFFFIWQVPHFWLLMLKYGEEYTKAGYPSITSVYSSRQIKHITFIWILATAVS
                     TLMLPAFNVVESWVVMGGLLISSMWLVGQFTRLLSLKEMPFSPIRYFMKINYFVLAVI
                     IFLSIDRFI"
     gene            complement(2592..3497)
                     /gene="coxB"
                     /locus_tag="PKZ74_12870"
     CDS             complement(2592..3497)
                     /gene="coxB"
                     /locus_tag="PKZ74_12870"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPM93693.1"
                     /translation="MSGASNFVHGVDLTFIIILGISLFFLVGITAVMIYFVIRYSRKR
                     NPKATNIEGNTKLEILWTVIPLILVLVMFWFGWVGYQPMRKVPEGAIPVKAIGQMWSW
                     SFEYPNGKKSAELVVPLNKPVKLDLFSRDVLHSLYIPAFRIKEDVVPGKNNYMWFTAL
                     ELGEYDLYCAEYCGERHSYMLSKVKVLPEAEYESWLVQSDIPADEHPGLSVLKQNGCI
                     ACHSLDGSKIVGPSFKGIYGHEVEVITDGKERKIIVDDEYILKSIYEPNADVVKGFNP
                     GLMISYKEQLKEEDVKKIIEYIKGL"
     gene            complement(3508..3828)
                     /locus_tag="PKZ74_12875"
     CDS             complement(3508..3828)
                     /locus_tag="PKZ74_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HPM93694.1"
                     /translation="MNQHPNQHKEHSAEVHIVSYREHASTFLALILLTVMTVTVSVFG
                     ADLYTLTVLTALAIASAKAVVVGLYFMHLKYDPKIYKAMMAVVLILFTVFLILTLIDY
                     LIRP"
BASE COUNT         1037 a          994 c          931 g          974 t
ORIGIN      
        1 aagggtctac ggcgcattcc attccgtcgc cgcccaggat gaactgccag tagttaccca
       61 tgtaggtcga tgtgccattg tcctggtacc cgttgaccgt tttatcgcgc atcgtggcgc
      121 tttgcccgat cttgtatacc tgtccgatag ggaggccatt ggtaatttca gtccatgttt
      181 caccctggtc gtctgtgtag taaaccccac cgtcattacc agcatacagc ctgtcgttca
      241 gtggattgta ttcgagcaca tgcaaatcgg catgcactga cggaaccccg cagtcgcccc
      301 accaatggga gctgatcgac cagatcgatc cgccggtaac cgatttaaag caattgactc
      361 caccggcaaa gatcacatcg gcatcattcg gatcgcaggc aatgtcgagg tcgtaccagg
      421 cttgtccgcc atcgccgccg ttacagcccc aactcataat gttaggggaa ttcgacatca
      481 tggtgaaatt caggccggcg tcggttgacc ggtacaaacc catgaaggaa tcaccgtatg
      541 tcaggattac ataaacgtaa ttaggattgt cgggggtaac ggcaatagcg gatcgcgaac
      601 ttcccggaag gccgctggta atctgggtcc agttcagccc gttgttggaa gaacggtaaa
      661 aattgccgtt ggccgcggcg taaacgatat cagggttatt cggtttaaag accacatctt
      721 tgaaatcgcc cgtctgcacc cgggtccagg tagctccgcc attggtggtg cggaatagtc
      781 ctccgctcgt tgcggcaaag atgatcatat ggttagaggg atgcatcagg agccgtccga
      841 caacgtaatt cgtcattccc aggttccatg gttcccatgt gtccccgccg tccgtcgaac
      901 gatacacgcc aattccgtaa gcatcgccgg catcccggtc gcctgtgccg atgtacatca
      961 catccggatt ggtcggatcg atcagaatgg ctgatacacc cagcgaaggc agttcctgtg
     1021 tcgaagaggt ccattcaccg ccatgcgtgg tggttttcca taagccgccg gagggcgaac
     1081 ccacccaaac gatatcagga tcttccgggt caaaagcgat ggcgtttaac cttccaagcc
     1141 ctttatagcc tttgccgtca ggaagtacga aaggtcccag gttggtccag tcgccggcct
     1201 gtgaccgggc tcccggatgg gttgccaggt atgattcata agccttaacg atatgatcct
     1261 caggtcttcg tgttccgtcg ggaaatatcc ggccctggag catgctgtat tcccaccttt
     1321 taaagggttt ccaacccttc agcttggtca ggtcttttcc ttcataatac gtattaaatg
     1381 cctcctgaac atcataaaaa ttcacagacg catccgacat catttcgatc cagtacggat
     1441 agctggagta atcggatata ccgggagaaa cattttgtgc acgtgattga aaaataaaca
     1501 ggatgcaaaa aacggaaaga gtgaataaat atctcatctt cattaatata aatcaaaata
     1561 taggcaaatg ccggattcca aaaatacaca tttttaatta ctgaatgtat ttaaggttaa
     1621 aaaacgttcg gaaaaaaaac ggtttagatg aaccggtcga tactgagaaa gataatcaca
     1681 gcaagtacga aataattgat tttcataaaa tagcggatgg ggctgaaagg catctctttc
     1741 agggaaagca gccgggtaaa ctgtccgacc agccacatgg aagagatcaa taatcccccc
     1801 ataacgaccc atgattcgac gacattaaaa gcggggagca tcagggtgga aacggctgtg
     1861 gccagaatcc agataaaagt gatatgttta atttgccggg atgaatacac tgacgtgatg
     1921 gatggatagc cggctttggt gtactcttcg ccgtatttta gcatcaggag ccagaagtgg
     1981 gggacctgcc agatgaaaaa gaagaaggcc atgaacatcg ccctgggatc cgtaaggctt
     2041 ccgccggctg caacccagcc gaccagggga ggtattgcgc cgatgaccgc tcccggaact
     2101 actgcaaaag cgctcttttt cttcagagga gtgtaaatga cgttgtacca gatgagcgcc
     2161 aggataccca attgcatggc cagtatgccc gaaccgatgt aaagcaaacc agagccggcc
     2221 atcgacataa gaaaagcaaa agctaatgca gcccccggag agatccttcc tgaagggatc
     2281 gggcggtgac gggtccgctc catcagggcg tcacgttctc tctcctggta gtgattgatg
     2341 gccgacgaac cgcaggccag cagaaaaata cccaatgtcg gcaaaatcaa tcccgaatca
     2401 aacgacccgc cacccagcag gtaccccgtg atcgtcgtca gcgccaccgc aaacgtgatc
     2461 tttacctttc ccagctcaag atatgaattg atcagtggca ttgttcaatt gttcaattgt
     2521 tctattgtta tttttttcca gtctgaccgt cctactgtct taacatctta ccgtcatacc
     2581 gtcataccgt cctaaagtcc tttaatatac tcaattatct tcttcacatc ctcctctttc
     2641 aactgttctt tgtaggagat catcagcccc ggattgaatc cttttaccac atctgcattc
     2701 ggctcataga tcgattttag tatgtattca tcatcaacaa taatcttcct ctcttttccg
     2761 tcggtaatca cctcaacctc atgcccgtat atacctttga acgaagggcc tacgatctta
     2821 ctgccgtcga gtgagtggca ggcaatacaa ccattctgct tcaaaacgct caaccccgga
     2881 tgttcatcgg cagggatatc cgattgaacc agccaggatt catattccgc ttcaggcaat
     2941 acttttactt tgctgagcat gtaggaatgg cgttcgccgc agtattcggc gcaatagagg
     3001 tcgtattcgc ccaattccag ggcagtaaac cacatatagt tgtttttacc ggggaccaca
     3061 tcttccttga tacggaaagc cggaatgtaa aggctgtgaa gcacgtccct ggaaaacagg
     3121 tcgagtttga ccggtttatt cagcggtacc accagttccg ccgacttttt accgtttgga
     3181 tattcaaatg accagctcca catctggcca atggctttaa caggtatggc gccttccggt
     3241 acctttctca tcggctggta tccgacccat ccgaaccaga acatcaccag caccaggatc
     3301 agcgggatga ctgtccaaag gatctcgagt tttgtatttc cctcgatgtt ggtggctttc
     3361 ggatttctct tccggctgta ccggatgaca aaatagatca ttacagccgt gatcccgacc
     3421 aggaaaaata acgagatccc caggataatg atgaaggtca gatcgacccc atgaacaaaa
     3481 tttgatgctc cggacatgtc tggcaattta aggtctgatt aaataatcga tcaacgtcag
     3541 aatcaggaaa acggtgaaca agatcaacac aactgccatc atggctttgt agatcttcgg
     3601 atcgtatttc aggtgcataa aatacaaccc taccactact gctttagccg aagcgatggc
     3661 cagggccgtt aaaacggtta atgtataaag gtcggcgccg aaaacggaaa cggtaacggt
     3721 catgaccgtc agcaggataa gtgccagaaa cgtgctggca tgctcacggt aactcacaat
     3781 atgcacttcg gcggaatgct ctttatgttg atttgggtgt tggttcatga tagctctgtt
     3841 ctattgttct attgttctat tgtttcattg ttaccgtcct aacgttctaa tggatcaggt
     3901 aaaacagcgg gaaaaggtag atccagatca gatcga
//
LOCUS       DAOPNH010000298         3923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27452_length_3923_cov_6.630558, whole genome shotgun sequence.
ACCESSION   DAOPNH010000298 DAOPNH010000000
VERSION     DAOPNH010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27452_length_3923_cov_6.630558"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..251)
                     /locus_tag="PKZ74_12880"
                     /pseudo
     CDS             complement(1..251)
                     /locus_tag="PKZ74_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(316..831)
                     /gene="trxA"
                     /locus_tag="PKZ74_12885"
     CDS             complement(316..831)
                     /gene="trxA"
                     /locus_tag="PKZ74_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM93695.1"
                     /translation="MKKPGYLIIFSTLLFISCSGTSNEKPANAAVNNTVQTKDGGDEQ
                     KETAAGKTIHLNAQTFKAKVMNYEKNQQQWVFEGDKPAIVDFYADWCRPCRMIAPIME
                     ELAAEYEGKIDIYKVDTDDERELAAVFGIQSLPSVLFIPMTGKPSLQKGAMSKEAYKQ
                     IIDDFLLKQQQ"
     gene            939..1346
                     /locus_tag="PKZ74_12890"
     CDS             939..1346
                     /locus_tag="PKZ74_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM93696.1"
                     /translation="MGFGFKDISQDILNNVLNDEDRCLAFIAEEKWRDGFMCRKCGHT
                     NYCKGKKPHSRRCTRCKQEESATSHTIFHGCHLPVTQAFRLAYQVCHNPSISTYELSR
                     QLETRQMTCWKLKKRMLECIETQGTLTVIPPNL"
     gene            complement(1364..2059)
                     /gene="radC"
                     /locus_tag="PKZ74_12895"
     CDS             complement(1364..2059)
                     /gene="radC"
                     /locus_tag="PKZ74_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPM93697.1"
                     /translation="MFYESRIPIKQWSEDDRPREKLLLKGKSALSNAELIALLLGSGN
                     RNESAVDLAKRVLDKSGQNLAELSRFQISDLIKIKGIGQAKGLTIIAALELGNRKRSQ
                     DALSREKVAGSHDVYELFHGELADAQYESFWVLLLNRANRVIRKVNISEGGISGTVAD
                     PKKIFKMSLEHNASSLILCHNHPSGNTQPSEADIRLTRKLKEAGLLLDLPVLDHLIVG
                     ADSYFSFADEGIL"
     gene            complement(2220..2474)
                     /gene="rpsT"
                     /locus_tag="PKZ74_12900"
     CDS             complement(2220..2474)
                     /gene="rpsT"
                     /locus_tag="PKZ74_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPM93698.1"
                     /translation="MANHKSALKRIRANENRKIQNRYHGKTMRNAIKKFRSIEAKEEA
                     TAQLPKLVSMIDKMAKRAVIHKNKAANLKSKLTRKVNAIA"
     gene            2630..>3923
                     /locus_tag="PKZ74_12905"
     CDS             2630..>3923
                     /locus_tag="PKZ74_12905"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM93699.1"
                     /translation="MHKKIDLLFALLLVSLAAFSQDKTPSFITDSLDNYIERSLRDWN
                     IPGAAVAVVHNDRVVVTKGYGFLEMGKKDKVDENSLFMIASNTKAFTGTAIAILESEG
                     KCSMDDRVQKYLPGFRMKDPWVAEHLTLTDVLSHRIGMETFQGDFMYWESELTSDQVV
                     EKFGQLTPVYDYRAKWGYCNAGFLIAGMCLEAMTGASWDAFIRTRFLQPLEMNRTLAL
                     TSEVKNATNLASAHTLVDGKLQVIPHCRIDNLAPAASISSSVSDMSHWLIALLDSGRY
                     GGRQVIPFKAIKSAQTPRSFVRKAGHPFNTAHYSLYGLGWDLRDYEGVEIVSHTGGVD
                     GFVTSVTLIPEQDLGIVVLTNTDANGFYEALKWEIIDAYMDFPYRDYSRFYLNRYKFF
                     QEQDLDQVNAWRDSVVLKLPLPVEMSRFEGRYVDEVYGY"
BASE COUNT          927 a          979 c          931 g         1086 t
ORIGIN      
        1 ctgcggatac cgaaaacgcc ggccagttcc tgctgctctt ccgtgtctac cttatatata
       61 ttgatcttcc ctttgtactc ctcggccagt tcctccagta tcggggctac catcttgcac
      121 ggtccgcacc agtcggcgta aaaatcgatc aggcacggca gctctccggc aaaattccat
      181 tccttgtttt tttcgtaatc aaataccttg ttcagaaaag tatcttttgt caaatgttcc
      241 agcttcatgt taatgttaat gttaatgtta atgttaatgt taatgaacta atgcatatta
      301 ttcccttttc cctgcctact gttgctgctt cagcaagaag tcatcgatga tctgtttata
      361 agcctctttc gacatagctc ctttttgcaa cgacggcttg ccggtcatcg gtataaagag
      421 caccgaggga aggctctgga tgccgaatac ggccgccagt tcgcgctcat cgtcggtatc
      481 caccttataa atatcaattt tcccttcata ctcggcggcc agttcctcca tgatcggcgc
      541 aatcatccgg caggggcggc accagtcggc gtaaaagtcg acgattgccg gcttatcgcc
      601 ttcaaaaacc cattgctgct ggttcttttc gtaattcatc accttggctt taaaagtctg
      661 ggcattgaga tgaatggtct ttccggcagc agtttccttt tgttcgtcac ctccgtcttt
      721 tgtctgcacg gtattgttta cagcggcatt ggccggtttt tcgttggatg ttccgctgca
      781 gctgatgaac agcaaggtcg aaaaaataat gagatatcct ggttttttca ttttcttttt
      841 attttttaaa tcatgcggca aaggtaaaat atattttgat attaaagcgc attagtatca
      901 ctattttttt tatttttgtc aaaaattttt ttacccgcat gggatttggt tttaaagaca
      961 tatcacagga tatattaaat aatgtattga acgatgagga ccgctgcctg gcattcattg
     1021 cggaagaaaa atggcgcgat gggtttatgt gccggaaatg cgggcataca aattactgca
     1081 aaggcaagaa gccccattcc aggagatgca cccggtgcaa gcaggaagaa tcggccacat
     1141 cgcacaccat ttttcatggc tgccatctgc cggttacaca ggcttttcgc ctggcttacc
     1201 aggtttgtca taatccttcc atctcaacct atgagctttc ccggcagctt gaaacccgcc
     1261 agatgacatg ctggaagctg aaaaagagaa tgctggaatg cattgaaacc cagggcaccc
     1321 tcactgtcat ccctccaaac ttataactcg caacccaaac tcactagagt attccctcat
     1381 ccgcaaaact aaaatagctg tcggcaccga cgatcaggtg atccagcacc ggcaggtcga
     1441 gcagcagccc ggcctccttc agtttccggg tcagccggat atccgcttca ctcggctggg
     1501 tgttgccgct cgggtgatta tggcagagga tcagtgagct ggcattgtgt tccagcgaca
     1561 tcttgaagat cttcttcgga tcggccacgg tacccgagat ccctccttcg ctgatgttga
     1621 ctttccggat gacccggttt gcccggttca gcagcagcac ccagaatgat tcatactgtg
     1681 cgtcggccag ctcgccatgg aacagctcat acacatcgtg gctaccggcc actttctccc
     1741 ttgatagcgc gtcctggctt cgtttacggt tgcccagttc cagcgccgct atgatcgtca
     1801 gccctttggc ctgcccgatt cctttgatct tgatcaaatc gctgatctgg aaccgcgaaa
     1861 gctccgccag gttttgcccg cttttgtcca ggaccctttt agccaggtcc acggccgact
     1921 cattccggtt gcctgaaccc agcagcaaag cgatcagttc ggcattgctc agagccgatt
     1981 tgcctttcag caatagcttc tccctgggcc ggtcatcttc cgaccattgc ttgattggaa
     2041 ttcttgattc gtaaaacata cccttttttc ctgtttatcc ggaaaaaggg ctgaaaatac
     2101 ccggtcgtgg taaaaaaaag ttttatgacc cggataacaa attgatttat aataacaaat
     2161 ggaaataaaa aaagctgcct gtcgggcgac aggcagccgt aaaacagtct gaaaattctt
     2221 taagcgatag cgttgacttt gcgggtcagt ttcgacttca ggttcgccgc tttgttcttg
     2281 tggatgacag cgcgcttggc catcttgtcg atcatcgata ccagcttggg cagttgggca
     2341 gtcgcctctt cctttgcttc aatggatctg aatttcttaa tggcattacg catggtcttg
     2401 ccatggtaac gattctggat tttcctgttt tcattggccc tgatcctctt cagcgccgat
     2461 ttatgatttg ccatgtttta gcgttttata ttccaattgg gggtgcaaag gtatagatta
     2521 tttttacaat agaaaaagtt ttatgaaaat atttgaaaag actgggtgct gataccggaa
     2581 gccaaaggaa aaattaacgg gttatgatta tttttaccga aaaaaaagta tgcataaaaa
     2641 gattgatttg ctgtttgcat tactcctggt gagcctggct gctttttctc aggataaaac
     2701 accttctttt ataactgaca gcctggataa ttacatcgaa cgatcactca gggattggaa
     2761 tatcccgggc gcggccgtgg ccgtggtgca taacgacagg gtggtggtca caaaaggtta
     2821 tggttttctt gaaatgggaa aaaaggataa ggtcgacgaa aactcacttt tcatgattgc
     2881 ttccaatacc aaggccttta ccggaacggc gatcgcaatc ctcgaatcgg agggtaagtg
     2941 ctcgatggat gaccgggtgc agaaatacct tcccggtttc cggatgaaag acccctgggt
     3001 ggcggagcat ctgacactca ccgatgtact gtcgcaccgg atcggaatgg agaccttcca
     3061 gggtgatttc atgtactggg aatcggaact gaccagcgac caggtcgttg aaaaattcgg
     3121 acagcttaca cccgtctacg attaccgggc aaaatggggc tactgcaacg ccggcttcct
     3181 cattgccgga atgtgccttg aagctatgac cggagcaagc tgggatgctt ttatccgaac
     3241 ccgcttcctg cagcccctgg agatgaaccg tacactggcc ctgacaagcg aagtgaaaaa
     3301 tgccacgaac ctggcttccg cccatacact ggttgatggc aaacttcagg tcattccgca
     3361 ctgccggatt gataacctgg ctccggctgc cagcatcagt tcgtcggtca gcgatatgag
     3421 ccactggctc atcgccctgc tcgacagcgg ccgctacgga ggccggcagg tcatcccttt
     3481 caaagccatt aaatcggcgc aaaccccgcg atcattcgtg agaaaagctg gccacccgtt
     3541 caataccgcc cactattctt tgtatggcct gggatgggat ctgcgcgatt atgaaggcgt
     3601 tgagatcgtg tcgcatacgg gtggtgtcga tggatttgtc acttctgtta ccctgatccc
     3661 ggagcaggac ctggggatcg tcgtgctgac caataccgat gccaacggct tttacgaagc
     3721 gctcaaatgg gagatcatcg atgcctacat ggattttcct taccgcgatt atagccggtt
     3781 ctatctgaac cgttacaaat tcttccagga acaggacctg gatcaggtga atgcctggcg
     3841 cgattcggtt gtgctgaaac tgcctcttcc ggttgaaatg agccgttttg aaggacgata
     3901 tgttgatgag gtttatggct atg
//
LOCUS       DAOPNH010000299         3922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27461_length_3922_cov_5.925782, whole genome shotgun sequence.
ACCESSION   DAOPNH010000299 DAOPNH010000000
VERSION     DAOPNH010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27461_length_3922_cov_5.925782"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2957
                     /locus_tag="PKZ74_12910"
     CDS             <1..2957
                     /locus_tag="PKZ74_12910"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93700.1"
                     /translation="KNHNVVWAGTGENNHQRALGYGDGVYKTTDGGKSWTNMGLKNSR
                     QIGMIAIHPENTDVVFVAAEGSVWGPGGDRGLYRTSDGGKTWEKVLDISENTGVNNVL
                     IHPENHNIMFATSEQRRRHVFTKIGGGPETAIYKSTDGGKTWNKLSSGLPGGDMGGIG
                     IAISPVNPAYIYAIIEAEGESGGFFRSTDCGESWSKMSDHTAQGQYYNEIICDPKDVN
                     KVYSMETVSHVTEDGGKTWQSVGLDNRHVDDHAMWIDPSDTEHFFIGGDGGVYETFDG
                     GDHYFFKTNLSVTQFYRVNVDDTEPFYWVYGGTQDNNSLGGPSQNISYAGVSSYEWIP
                     TLGGDGFFQAIEPGNPDIVYSAYQYGNIYRFDKKSREGIKIRPEPVKGEETYKWNWNT
                     PFILSPHSPTRLYIAANKVFRSDDRGQSWKIISGDITTQTDRNTWPVMGRYWGIDAVA
                     KDVSTSPFGTAISLAESKIKENLLYAGTDDGVIQVTEDAGRNWTKITRFPGVPEYTLV
                     SDILPDRFDENVVYACFDNIKRDDFKPYLLKSTDKGKTWTSIAANLPENHTVLTIAQD
                     FIDKDILFAGTEFGVFFTLDGGKIWTQLNAGLPTIKVPDIVIQERECDLVIATFGRGI
                     YILDNYSPLRQLKKETREKEAFIFPVKDALMFSRDGGYYGQGSNFFYAPNPEYGATIT
                     YYLKDVPKTLKDIRKEKESKLAEEKKPIPIPTLEEMRAEENELPPYLLFTIKDMSGNT
                     IRKMAASPSKGTSRMTWDLRYANGYNVDLDGDKFNPYSAGNGGFMAMPGNYTVSMAMV
                     FNGEVKELAGPVEFTARVLNNTTLPNADRAGLVAFQDKVSRMINAVNAADEVAKELMK
                     RTQYVRQSIQNTPGAPFGMMETAAALEKKLGDILWALHGEEPKASDEENRPAPPPVMM
                     RLGAIMEASWGNTSSPTQTQRDQLALLEEELPPLLQQLRQIAETELKDLESELDKLGA
                     PWTPGRIPEWKK"
     gene            3094..>3922
                     /locus_tag="PKZ74_12915"
     CDS             3094..>3922
                     /locus_tag="PKZ74_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93701.1"
                     /translation="MKYLALLFLFVATLVSAVIAIHLIINKPAPETGKIPNDWFFLQR
                     SFPYPEINYDARAMAWKQSQTLREESAARGEGWLLKGPLNIGGRIAAMAMITYGTQSV
                     YAGAASEGIFKSSDLGQSWRPIFDEAMSLSIGDIAIAPSDTSVIWVGTGEANAGGGSM
                     AYDGNGIYLSWDAGLSWSPVGLNNSGSIGRLVVHPDDPQTCYVAAMGRLFSDNPERGI
                     FKTSDGGGTWDQVLYINDSVGAIDIVMHPAKPDTLYAAMWERVRRPHRRDYGGPGSGI
                     "
BASE COUNT         1014 a         1047 c         1047 g          814 t
ORIGIN      
        1 cgaagaacca taacgtggtc tgggccggta ccggcgaaaa caaccaccag cgggcgctgg
       61 gctatggcga tggcgtctat aaaaccaccg acggcggtaa aagctggacc aatatgggac
      121 ttaagaattc ccgccagatc gggatgatcg ctatccaccc cgaaaatacc gatgtcgtgt
      181 tcgtggcagc cgaaggatct gtctgggggc ccggcggcga ccgcggcctt taccgcacca
      241 gcgacggtgg aaagacctgg gaaaaggtac ttgacatcag cgaaaatacc ggcgtgaaca
      301 atgtgcttat tcacccggaa aaccacaata ttatgttcgc cacctcagaa cagcgccgcc
      361 ggcatgtgtt tacgaaaatc ggcggaggcc ccgaaaccgc gatctataaa agcaccgacg
      421 gcggcaaaac ctggaataag ctttcttccg gattacccgg cggcgatatg ggcggtatcg
      481 ggatcgccat ctcaccggtc aatcccgctt acatctatgc catcatcgaa gccgaaggcg
      541 aatcgggcgg atttttccgc tctaccgact gcggcgaatc gtggtcgaaa atgagcgacc
      601 atacggccca gggtcagtat tacaacgaaa taatctgcga tccgaaggat gtgaataagg
      661 tctattcgat ggagactgtt tcgcatgtta ctgaagacgg aggcaaaacc tggcagtcgg
      721 tcggcctgga taaccgccat gtcgatgacc acgccatgtg gatcgaccct tctgataccg
      781 aacacttttt catcggcggc gatgggggag tatacgaaac gttcgacggc ggtgatcatt
      841 atttcttcaa gaccaatctg tcggtaaccc agttttaccg ggtcaatgtc gatgataccg
      901 aacctttcta ctgggtttac gggggaacac aggacaataa cagcctcgga ggacccagcc
      961 agaatatcag ttacgccgga gtttcatcgt acgaatggat ccccaccctg ggtggcgacg
     1021 gattcttcca ggccattgaa ccgggcaacc ccgatatcgt ttattcagct taccagtatg
     1081 gcaatattta ccgatttgat aaaaagagcc gtgaagggat caaaatacgc cccgaacccg
     1141 ttaaaggcga agagacttat aaatggaact ggaatacgcc gttcatcctc agcccgcatt
     1201 ctccaacccg tctttatatt gcagccaata aagtgttccg cagcgacgac cgagggcaga
     1261 gctggaaaat catcagcggc gatataacca cgcaaaccga ccgcaatacc tggcctgtca
     1321 tgggccgcta ctgggggatc gatgccgtgg ccaaggacgt ctccacatca cccttcggaa
     1381 cagccatatc actggccgaa tcaaagatca aagagaattt gctctacgcc ggtaccgacg
     1441 atggcgttat ccaggtaacc gaagatgcag gccggaactg gacgaagatc accagattcc
     1501 ccggtgtgcc ggaatatacc ctcgtcagtg atatccttcc cgaccgtttc gacgaaaatg
     1561 tggtctatgc ctgcttcgac aacatcaagc gcgatgattt caaaccctat ctcctcaaaa
     1621 gcacggataa gggaaaaacc tggacctcca ttgccgccaa ccttccggaa aatcataccg
     1681 tcctgacgat tgctcaggat ttcatcgaca aagacatcct atttgccggg accgagttcg
     1741 gtgttttctt taccctcgac ggagggaaga tctggacaca actgaatgca ggcctgccca
     1801 cgatcaaagt gcccgatatc gtcatccagg aaagggaatg cgatctcgtc attgccacct
     1861 tcggccgcgg tatctacatt ctggataact attccccgct tcgccagttg aaaaaggaga
     1921 cccgcgagaa ggaggccttt atcttcccgg tcaaagatgc cctgatgttt tcccgcgacg
     1981 ggggttatta cggccaggga tccaatttct tttatgctcc caacccggaa tacggcgcca
     2041 cgatcaccta ctatctcaaa gatgtgccaa aaacattgaa ggatatcagg aaagaaaaag
     2101 aatcgaaact tgcggaagag aagaagccca tcccgatccc gaccctggaa gagatgaggg
     2161 ccgaggagaa tgaattgccg ccgtatcttc ttttcacgat taaagatatg agcggaaata
     2221 ccatcaggaa gatggcggca tcgccctcca aaggcacctc gagaatgacg tgggatttgc
     2281 gctatgctaa cggctataac gtcgatctcg acggggacaa attcaatcca tacagcgccg
     2341 gtaatggcgg atttatggcc atgccgggaa attatacggt ttcgatggcc atggttttca
     2401 acggagaggt gaaagaactg gccgggccgg tagagtttac cgcccgtgtg ctgaacaata
     2461 cgaccctgcc gaatgctgac agggccggcc tggtcgcttt ccaggataag gtttcccgga
     2521 tgatcaatgc cgtgaatgca gccgacgaag tggccaaaga gctgatgaaa aggacacagt
     2581 atgtcagaca atcgatccag aacacgccgg gcgccccgtt tggcatgatg gaaacggctg
     2641 cggccctgga aaagaagctg ggcgatatcc tttgggcgct tcatggcgaa gagccgaaag
     2701 ccagcgacga agagaaccgc cccgccccgc caccggtcat gatgaggctg ggagcaatca
     2761 tggaagcctc ctggggcaac acctcctcac cgacgcaaac ccagcgcgat cagctcgctc
     2821 tgcttgaaga agagcttccg ccgctgctgc agcaactgag gcaaatcgct gaaacggagt
     2881 tgaaagacct ggaatcggaa ctcgacaaac tgggcgcccc ctggacaccc ggccgcatcc
     2941 cggagtggaa gaaataaatg catcagattg ctctgattga gcaacttcgt gtaacttcgt
     3001 gacttcttcg tgatcttcgt gtaacaagtt tttaaactaa agtatttcac gaagttcacg
     3061 aaggatacac gaagatccac gaagaaaaac aaaatgaagt atttagcatt actattcctc
     3121 tttgttgcta cgctggtttc tgccgttata gcgattcatc ttatcatcaa taaacctgca
     3181 cccgaaaccg ggaaaatccc gaacgactgg ttcttccttc agcgttcctt tccgtacccg
     3241 gaaattaatt acgatgccag ggcaatggcc tggaagcagt cgcaaacact acgggaagag
     3301 agcgctgcaa ggggtgaggg atggctttta aaaggcccgt tgaacattgg cgggcggatt
     3361 gcggcgatgg ccatgatcac ttacggaacc caatcggttt acgccggcgc agcatccgag
     3421 ggcattttca aatcatctga cctgggtcaa agctggaggc cgatattcga tgaggccatg
     3481 agcctttcca tcggcgatat tgccattgcc ccgtcagata cctcggtgat ctgggtcgga
     3541 accggcgaag ccaatgccgg cggcggttcc atggcatacg acggcaacgg tatctatctt
     3601 tcgtgggatg cagggctttc ctggagcccg gttggcctca ataattccgg gagcatcggc
     3661 cgattggttg tacacccgga tgatccccaa acctgttatg tggcggcaat gggccgtttg
     3721 ttctcggata atccggagcg gggaatattt aaaaccagcg acggaggcgg tacctgggac
     3781 caggtcctct acatcaacga ttcggttggc gctatcgata ttgtcatgca tccggcgaag
     3841 cccgataccc tttacgccgc catgtgggaa agggtgcgcc gaccgcaccg gagggactat
     3901 ggcgggcccg gcagcggtat ct
//
LOCUS       DAOPNH010000300         3912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27560_length_3912_cov_6.146746, whole genome shotgun sequence.
ACCESSION   DAOPNH010000300 DAOPNH010000000
VERSION     DAOPNH010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27560_length_3912_cov_6.146746"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKZ74_12920"
     CDS             complement(<1..500)
                     /locus_tag="PKZ74_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93702.1"
                     /translation="MKRKIIFGAIIFSIAFAVLFSLMYKWGQTGNPFRAETVVYGAVI
                     LLNLLITGSVGYVIFRKFSKKASGQLEKFIIPAGISFVLFALIVSLSLVSLGVYLLYL
                     IRGNDMTNFAGQLFTVELVSALKQFSAWILIGSALFFYMIWRKAIEREQLLREENLKY
                     KYRNLKS"
     gene            complement(519..1352)
                     /locus_tag="PKZ74_12925"
     CDS             complement(519..1352)
                     /locus_tag="PKZ74_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93703.1"
                     /translation="MKTSKILLMALLLVSNGLGAQKNSIDRYLSLKPSEIKARKAEIQ
                     EYAVTLKWQNLDAIHGNKFNCDAVSATYIVGLENDSVSWKDVSSAQISDFLQTDYSGA
                     SLPSFNGFTYKINETNFLNEDFYESIPVEQRDLAKWLVSDAVQMQGLAWYIFDSLEFN
                     KPFQMKFLNNYEIKFEDWVTFTSRYQKFIWSGITKYNNEVCAIIRFESLYNPLKIDNS
                     QMSVLGRALYYGEMWISLEDKQVEYATMVEDDVLKLKSPLFPEEQLIDLQREVIFKKK
                     E"
     gene            complement(1540..3324)
                     /gene="uvrC"
                     /locus_tag="PKZ74_12930"
     CDS             complement(1540..3324)
                     /gene="uvrC"
                     /locus_tag="PKZ74_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPM93704.1"
                     /translation="MDTSLVLRTLPDRPGVYQFYDQEGKLLYVGKAKNLKKRVSSYFN
                     REHFGGKLNMLVRKTAEIRHIVVGSEFDALLLENNMIKEHQPRYNVMLKDDKTYPWIC
                     IKNEPFPRVFPTRTIVKDGSLYFGPYASVKMMNTLLELIRQIYPLRTCSLKLTKQNID
                     AGKFSVCLEYHLGNCLGPCAGLQAEENYMAAFAGIREMIKGNFSGVLKELRARMESHA
                     QAYEFEKAQAVKEKISLLERYQAKSTIVNPKLGEMDVFSIADGEEEAYVNYMKVANGA
                     VIQAHTVELKKKLDESLEELLAYVVFDLRTRLASEAREILVPFELDLELPGVILTVPK
                     IGDKKHLLLLSEKNAKYYKLEKEKQLDLVDPERKVNRIMATMMKDLRMKEPPVHIECF
                     DNSNFQGDQAVSAMVCFRNGKPDKKEYRHFNVRTVAGPDDFATMEEVIMRRYKRLVEE
                     AKPLPQLIVVDGGKGQLSAALKSLEELGLRGKITIIGIAKKLEEIYYPDDPLPMYIDK
                     KSETLKILQRLRDEAHRFGITHHRKKREKATLKTELTEIRGIGEETAKKLLSDFKSVK
                     NIKNAPVEELEKSVGKAKAGVVFMYYHS"
     gene            complement(3408..>3912)
                     /locus_tag="PKZ74_12935"
     CDS             complement(3408..>3912)
                     /locus_tag="PKZ74_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPM93705.1"
                     /translation="LEREKAAFQGDPNLQPDLEGFDYILGRQLKPEPRTDIIRLLKEI
                     HVKPTAMIDISDGLGSEILHICKGSKLGCRIYEEKIPIDPTTVNMAGEFGIQPVTAAL
                     NGGEDYELLFTIRQEDFEKIRTIPNVSVIGHMTESAAGAHIVAPDGVLVEITAQGWNA
                     LKKNVEF"
BASE COUNT          937 a         1025 c          877 g         1073 t
ORIGIN      
        1 gatttcaggt tcctgtattt atatttcagg ttttcttccc gcagcaactg ttcccgttca
       61 atggctttac gccagatcat gtaaaaaaac agagcagagc cgatcagaat ccaggccgaa
      121 aactgtttca gcgcgctgac cagctcgacc gtaaacaact gcccggcaaa attcgtcata
      181 tcatttcccc ggatcaggta aagcaaatag actcccaggc tgaccaggga aagtgaaacg
      241 atcagcgcga acagcacaaa actgatgccc gccggaatga taaatttttc cagttgtccg
      301 gatgcctttt tcgagaactt ccggaatatg acatagccaa cactcccggt tatcagcagg
      361 ttcagcagga tgaccgcacc gtacacaacc gtttctgccc tgaacggatt gccggtctgt
      421 ccccatttat acatcagcga gaacagtacc gcgaaggcga tggaaaagat tatggcgccg
      481 aatattattt tccgtttcat ttccgtcaaa tatagcagtt attccttttt cttaaaaata
      541 acttcccttt gcaggtcgat caactgctct tccgggaaca acgggctctt cagttttaag
      601 acatcgtcct cgaccatggt ggcatattca acctgtttat cctccagcga tatccacatc
      661 tccccgtaat acaacgcccg gcccagaacc gacatctggc tgttgtcgat cttgaggggg
      721 ttgtataaag attcaaacct gattatagca cacacctcgt tattgtattt tgtgatgccc
      781 gaccagatga atttctgata acggctcgta aaagttaccc aatcttcgaa tttaatctca
      841 taattattta agaatttcat ttgaaagggt ttgttgaatt ccaggctgtc gaatatatac
      901 caggccaatc cctgcatttg aactgcatcc gacacaagcc actttgccag gtccctttgt
      961 tcaaccggaa tagattcata aaaatcctca ttgaggaaat tcgtttcgtt gattttataa
     1021 gtgaaaccgt tgaacgacgg caggctggcc ccactgtaat ccgtctgcag aaaatcgctt
     1081 atttgcgctg aactgacatc tttccagcta accgaatcat tttccagtcc cacgatatag
     1141 gttgcgctga ccgcatcaca attgaattta ttaccgtgga tggcatccag gttctgccat
     1201 ttcagtgtaa cagcatactc ctggatttcc gcttttcgcg ctttgatctc agacggtttg
     1261 agagagagat accggtcgat actgtttttt tgagccccca ggccatttga taccagcaat
     1321 aaagccatta ataaaatttt tgaagttttc atttttagaa aatttaagtt aataatcagc
     1381 aaaagaagcg gatgggattc cgattaaaaa atcaaagttg cagagttgct gattttcatg
     1441 gctgagtcgt caattaccgg aatcgggccg gttgtgaggg aaaagggaaa aagaaaaaag
     1501 aaaaaagaaa aagggaaaaa ggaatgactg ccgactgccc taagaatggt aatacatgaa
     1561 caccacccct gccttagcct tcccaaccga cttctcaagt tcctccaccg gggcattttt
     1621 gatattctta accgacttga agtcggaaag cagctttttc gccgtctcct ctcctattcc
     1681 ccggatttcg gtcagttcgg tcttcaaagt ggctttctcc ctttttttcc ggtgatgggt
     1741 gatcccgaag cggtgtgctt catcgcgtaa acgctgcaaa atctttaagg tctccgactt
     1801 tttatcgata tacatcggaa ggggatcgtc gggataatag atctcttcca gttttttagc
     1861 gatgccgatg atggttatct ttccgcgcaa cccaagctct tccaggcttt tcagcgcggc
     1921 gctgagctgc cctttcccgc cgtcgaccac gatgagctgg ggcaagggtt ttgcttcttc
     1981 caccagccgt ttatagcgac gcatgatcac ctcttccatc gtggcaaaat catcgggccc
     2041 tgcaaccgtc ctcacgttga aatgccggta ttctttttta tcgggtttcc cgttgcggaa
     2101 acacaccatg gccgataccg cctggtcgcc ctggaaatta gagttatcga aacattcgat
     2161 atggaccggc ggctccttca ttcgcaggtc tttcatcatc gtggccatga tccggtttac
     2221 ttttcgttcc ggatcgacca gatccagctg cttttctttt tcgagcttat aatattttgc
     2281 gttcttctcg gagagaagta aaaggtgctt tttatccccg atcttcggca cggtgaggat
     2341 cacgcccggc aattccaggt cgagttcaaa cgggaccagg atctcccggg cttcgctggc
     2401 cagccgggtg cgcaggtcaa aaaccacata cgccaggagc tcttccagtg attcgtcgag
     2461 ttttttcttc agctccacgg tatgagcctg gatgaccgcg ccattggcca ccttcatgta
     2521 gttgacatac gcctcttcct ccccgtcggc gatggaaaaa acatccatct ccccaagctt
     2581 cgggttcacg atcgttgatt tggcctggta gcgctccagc agggagatct tctctttgac
     2641 ggcctgcgct ttctcgaatt cataggcctg tgcatggctc tccattctcg cccgcaattc
     2701 cttcagtacc ccgctgaagt tgcctttgat catctcccgg atgccggcaa aagcggccat
     2761 atagttctct tccgcctgca acccggcaca gggcccgagg cagtttccca ggtgatattc
     2821 taaacagacg ctgaattttc ccgcatcaat attctgcttc gtcagtttca ggctgcaggt
     2881 gcgcaacggg tagatctggc ggatgagctc cagcagcgta ttcatcattt ttaccgaagc
     2941 atacggcccg aaatagaggg aaccgtcctt cacgatcgtc cgcgtcggga acaccctggg
     3001 gaaaggctca tttttaatgc agatccaggg ataggtcttg tcgtctttca gcattacgtt
     3061 gtagcgcggc tgatgctcct tgatcatgtt gttctccagc agcagggcat cgaactccga
     3121 tcccaccacg atatgcctta tctcggcggt tttccgcacc agcatattca gtttcccgcc
     3181 gaaatgctcc cggttgaaat acgacgaaac acgctttttc aggttcttcg ctttcccgac
     3241 atagagcagc tttccctcct ggtcgtagaa ctggtaaacc ccggggcggt caggcaaagt
     3301 cctcagaacg agagaagtat ccatacagat taatgttgaa tgttgagtgt tgaatgttga
     3361 atgattgatt aattattata aaattacccg aattcaaaat tcaacattca aaattcaaca
     3421 ttctttttaa gtgcattcca tccctgcgct gtgatctcca ccagcacccc gtcaggagct
     3481 acgatatggg cgcctgccgc ggattccgtc atgtgcccga tcaccgaaac attcggaatc
     3541 gtcctgatct tctcaaaatc ctcctggcgg atggtgaaca gcagctcgta gtcttctccg
     3601 ccgttcaagg cagcggtaac cggctggata ccgaattcac cggccatatt gaccgttgtc
     3661 gggtcgatgg gaattttttc ctcatagatc cggcagccca gcttcgagcc tttgcaaatg
     3721 tgcaggatct ccgatcccag tccgtcggat atatcgatca tagcggtcgg ttttacatga
     3781 atttccttca gcagacgtat gatatcggtc cggggttcgg gtttaagctg gcgccccagg
     3841 atataatcaa agccttcgag gtcgggctgt aaattgggat cgccctggaa tgccgccttt
     3901 tccctttcga gc
//
LOCUS       DAOPNH010000301         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_27857_length_3881_cov_5.444851, whole genome shotgun sequence.
ACCESSION   DAOPNH010000301 DAOPNH010000000
VERSION     DAOPNH010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27857_length_3881_cov_5.444851"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..688
                     /locus_tag="PKZ74_12940"
     CDS             110..688
                     /locus_tag="PKZ74_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93706.1"
                     /translation="MAKLYKNRYRIKSARCPNWDYGSKGAYFITICTKGRNHYFGEIF
                     NKTMILSKAGLIVDNCWHELPCHFPNIQIDAFIVMPDHVHLIIIIHQNPNKPGLAEDK
                     TTARNKDGTGEINQVIPEKDGYMSKISPKSGTVSTIVRSYKSACTNAINKSLPAINFG
                     WQPRYYDIIVRDYRSLDRIRRYIRLNPKYWKG"
     gene            752..1375
                     /locus_tag="PKZ74_12945"
     CDS             752..1375
                     /locus_tag="PKZ74_12945"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPM93707.1"
                     /translation="MTYTDYIPPDIKSLYEVHDFKHAAAILANEFPGEFEEICDAMRK
                     FRFSLNDIMEAGGSESHIPKIFSGILRPMEWKEKNLSAELKVYEVKGKSSELKSTINH
                     GSHMVDYLKGRVAFDLEWNSKDQTFDRDLYAFRAFFEYDAISVGVLVTRSNELDPLFR
                     KLGIMPKYGASTTHMGKLLPRLEAGRNGGCPVLVFGITTKLITDYEQ"
     gene            1365..2093
                     /locus_tag="PKZ74_12950"
     CDS             1365..2093
                     /locus_tag="PKZ74_12950"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HPM93708.1"
                     /translation="MNNDLSAADDFRRFAGGKKFATILADPPWQFENRTGKMAPEHKR
                     LARYSTLTLAEIKKIPVADAAAEAAHLYLWVPNALLSEGLEVMKAWGFNYKTNLIWYK
                     IRKDGGPDRRGVGFYFRNVTEMILFGVRGKNARTLQPGRTQENMIVKQKREHSRKPDE
                     QYDLIEACSPGPYLELFARGLRPKWECWGNQAEEYFPEWETYKNHSQKERKDRIAVPP
                     KIIDPKVPEQLKLLENIGKYKKKI"
     gene            complement(2202..2894)
                     /locus_tag="PKZ74_12955"
     CDS             complement(2202..2894)
                     /locus_tag="PKZ74_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM93709.1"
                     /translation="MKAKKAESANLENKRTIFLEIGMIITLVLVLCAFNWKTYDKKIV
                     TGFERGIDNTPVEMIPVTIQKQPELPQPKKPRVIHTINIIDDHIMVDEDYIVDAEIDN
                     MDTVPVYVPVKSLGDEESIVEDEIFRVVESAPEFPGGEEALYAFLRKNTEYPRLAREV
                     GISGKVYITFVVERDGRITDAQVVRGIGGGCDEEALRVVNMMPQWKPGLQRGHAVRVQ
                     FIMDIKFTLSQM"
     gene            complement(3012..>3881)
                     /locus_tag="PKZ74_12960"
     CDS             complement(3012..>3881)
                     /locus_tag="PKZ74_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPM93710.1"
                     /translation="DPLRGIADFKVGYSPERINPGDKEHTLITITKVVSGCDEESLDI
                     IARTYELVVKAGVHRAPSIKVAEAAKIIENTQRDVNIALMNELSIIFNRMGINTYDVL
                     EAAGTKWNFLRFFPGLVGGHCIGVDPYYLTFKAKELKYHPQVIDSGRFVNDSMGPYVA
                     KQLVKKLIAAGKNVMNSRILIMGTTFKENVSDIRNSKVADVYNELKSYGVHIDVVDPH
                     ANSGEVKHEYGYEVAKAPAGKYDAVIVAVAHKDYESLQESDFAALMNENGILVDLKGL
                     YRKRISRLTYWSL"
BASE COUNT         1052 a          903 c          923 g         1003 t
ORIGIN      
        1 tgttgataac cgttgttgtt gatattgtag agacgttgca tgcaacgtct ctacaatggc
       61 ggaaatggcg gaaatggcga aaatggcgga aatgtaaaaa atatcggaaa tggcgaaatt
      121 gtataaaaac cgatacagga tcaaatcagc gcggtgcccc aattgggatt atggttcaaa
      181 gggcgcatat tttatcacaa tatgcaccaa gggcaggaat cattattttg gggaaatttt
      241 caataaaacc atgatattgt cgaaggctgg tttgattgtt gataattgct ggcacgaatt
      301 accttgccat tttcccaata ttcagatcga tgcgtttatc gtcatgccag atcatgttca
      361 tttaataata attattcatc aaaatccaaa taaacctgga ttggcagaag acaaaacaac
      421 agcccgtaat aaagacggca ctggtgaaat caatcaggtt atacctgaaa aagatgggta
      481 tatgtcaaaa atatctccaa agtcaggaac ggtttcaaca atagtcagat catataaatc
      541 cgcatgtacg aatgcgatta acaaatcatt gccggcaata aattttggct ggcagccacg
      601 atattatgat attattgtcc gggattacag atcgcttgat agaattcgca ggtatatcag
      661 gctgaatccc aagtactgga agggatgaaa attaaataag cccaacggga tatttgtgta
      721 aatttggaat taattaaaat ggtattgaat aatgacttat accgactaca ttccgcccga
      781 catcaagtct ctttacgagg ttcatgactt caagcatgcc gcagctatcc tggctaatga
      841 atttccggga gaattcgaag agatttgcga tgctatgcga aagttcaggt ttagtttgaa
      901 tgatattatg gaggctggtg ggagcgaaag tcatattccg aagatatttt ccggaatatt
      961 gaggccgatg gagtggaaag agaagaacct gtcggctgaa ttgaaggttt atgaggtcaa
     1021 aggaaagtcg agcgaattga aaagcacgat caatcacggt tctcatatgg tagattacct
     1081 gaaaggacgc gttgcattcg atcttgagtg gaatagcaaa gaccagacct tcgaccggga
     1141 tctttatgcc ttccgggcct tttttgagta tgatgccatc agtgttggcg tattggttac
     1201 gcgcagcaat gaacttgatc cgctttttag aaagctgggt ataatgccca aatacggagc
     1261 cagtacgaca catatgggga aactgcttcc gcgtctggag gcaggacgga acgggggatg
     1321 cccggtattg gtttttggaa ttactacaaa attaattact gactatgaac aatgacctat
     1381 ctgcagcgga tgatttcagg agatttgccg gcgggaagaa gtttgcaacc atccttgcag
     1441 atccgccctg gcagtttgaa aaccgcaccg gtaaaatggc gccggaacat aaacgccttg
     1501 cgcggtattc taccttaacg ctggccgaaa taaagaaaat cccggttgcc gatgctgctg
     1561 ccgaagccgc tcatctctac ctgtgggtgc caaatgcgtt gttatcagaa ggccttgaag
     1621 tcatgaaagc atggggattc aactataaga cgaatcttat atggtataaa atccgtaaag
     1681 acggtggccc tgaccggaga ggcgttggct tttatttccg gaatgtcacc gaaatgatcc
     1741 tcttcggtgt tcgcggaaaa aatgcccgta ccctgcaacc cggccgtacc caggagaaca
     1801 tgatcgtcaa gcaaaaacgg gagcacagca gaaaacctga tgaacagtat gacttgatag
     1861 aagcatgcag tccggggcct tacctggaac tgtttgcccg gggtttgcgg ccgaaatggg
     1921 aatgctgggg caaccaggct gaagaatatt tccccgaatg ggaaacctat aaaaaccatt
     1981 cacaaaaaga aagaaaagac cgcattgccg tcccaccaaa aattatcgac ccaaaagtgc
     2041 cggaacagtt gaaattgctg gaaaatatag ggaaatacaa aaagaagatt taattgctga
     2101 tccaccccga aagtttgttt tacccttaat gttcacattt gcgctactct atagcttgaa
     2161 ttacaaaccc caacccttta ccccccttac aaccctcacc tttacatctg gctcagcgta
     2221 aacttgatat ccataatgaa ctgcaccctg acggcgtgtc cgcgctgcag cccgggtttc
     2281 cactgaggca tcatgttcac tacgcgcagc gcctcttcat cgcagccgcc accgatcccc
     2341 ctgactacct gtgcatccgt gatccggccg tcgcgctcca cgacaaatgt gatgtacacc
     2401 ttgccggaaa tgcctacctc tctggctaac cgtggatatt cggtgttttt cctgagaaat
     2461 gcgtacagcg cctcttcacc tcccggaaat tcaggcgccg attccaccac cctgaaaatt
     2521 tcgtcttcaa caatactctc ctcatcgccg agcgacttga ccggcacata aaccggaacg
     2581 gtgtccatgt tgtcgatctc cgcatccaca atataatcct catctaccat gatgtggtcg
     2641 tcgatgatgt tgatggtgtg gatcacccgg ggtttcttgg gttgcggcag ctcaggctgt
     2701 ttttgaatag taacgggaat catctcgaca ggcgtgttgt cgatgccccg ttcaaatcct
     2761 gttacaatct ttttgtcata agtcttccag ttgaaagcac acaagaccag gaccaatgtg
     2821 atgatcatcc cgatctccag gaaaatagtg cgcttgtttt caaggttggc tgattcggct
     2881 ttttttgctt tcatggctct taatattaag ggtttaatat tctaactata aatttagttt
     2941 atagaagagc cgaaatcaag gagaaagaag ttaaaggcag ttttgggtca ggagaaggcg
     3001 gatttattac cctacaaact ccagtaggtc agccggctga tccgtttgcg gtatagcccc
     3061 ttgaggtcga ccaggatgcc gttttcgttc atcagtgcag caaaatcact ttcctgcagt
     3121 gattcgtaat ctttgtgagc caccgcaaca atcaccgcgt cgtatttgcc tgccggggcc
     3181 tttgccactt cgtagccgta ttcgtgcttc acctcaccgg aattggcatg cggatcaacc
     3241 acatcgatat ggacgccgta cgacttaagc tcgttgtaga catcggcaac cttggaatta
     3301 cgaatgtcgc ttacattctc cttgaaggtc gttcccatga tcaggatgcg gctgttcatc
     3361 acattcttgc cggcggcaat cagcttcttc accagctgct tggcaacata cgggcccatc
     3421 gagtcgttca caaaccgccc gctgtcgatc acctgcggat ggtatttcag ctcttttgcc
     3481 ttgaaagtca ggtagtacgg atcgacaccg atgcaatggc cgccgaccag gccggggaag
     3541 aagcggagga aattccactt ggtaccggca gcttccagca catcgtaggt attgatcccc
     3601 atccggttaa agatgatcga caactcgttc atcagggcta tgttcacatc gcgctgggtg
     3661 ttctcgatga tcttggcggc ttctgccact ttgatgctcg gggcgcggtg aacgcccgct
     3721 ttcaccacca gctcatacgt gcgggcaatg atatcgagcg attcttcatc acaacccgat
     3781 actactttgg taatggtgat cagggtatgc tccttgtcgc cgggattgat ccgctccggg
     3841 gaatagccga ctttaaagtc ggctattccc cggagcggat c
//
LOCUS       DAOPNH010000302         3863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_28026_length_3863_cov_7.169643, whole genome shotgun sequence.
ACCESSION   DAOPNH010000302 DAOPNH010000000
VERSION     DAOPNH010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28026_length_3863_cov_7.169643"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..908
                     /gene="lipA"
                     /locus_tag="PKZ74_12965"
     CDS             54..908
                     /gene="lipA"
                     /locus_tag="PKZ74_12965"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPM93711.1"
                     /translation="MEENRGRKKPDWLKIKVPKGKEYIGVRDIIDRNRLHTICSSGQC
                     PNKEDCWGRGTATFMILGNICTRSCKFCAVPTGRPLPADEAEPARIAESVQKMKLKHC
                     VITSVDRDDLEDGGANIWAATIRAVKELNPATTIETLVPDFRGRKELLQIVIDAWPEV
                     ISHNLETVRRLTPQVRSAAIYERSLEVVGWVAESGIRSKSGIMLGLGETEEEVVQVMK
                     DLRRVGCEVVTIGQYLQPTAAHLPVKEYIRPEQFEKYRQTGLEMGFRHVESGPLVRSS
                     YHAEKHVL"
     gene            968..1669
                     /gene="lipB"
                     /locus_tag="PKZ74_12970"
     CDS             968..1669
                     /gene="lipB"
                     /locus_tag="PKZ74_12970"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPM93712.1"
                     /translation="MNFELKYEDLGLIPYRKAWDYQEKLFGGLMKKKLAGEKSFPGHL
                     LFCEHPPVYTLGKSGKKENLLLNEQMLERQGIEFYHINRGGDITFHGPGQIVGYPILD
                     LEQVSIGIREYIEGLEEAVILVLSRFGIKSGRLSGATGVWLDPEIPAKARKICAIGVK
                     ASRHITMHGFAFNVNTDLSFYRYINPCGFIDKGVTSLGKEMGAMQNIEEVKGLLKNCL
                     ESTLGISISEYPNLP"
     gene            complement(1673..2401)
                     /gene="recO"
                     /locus_tag="PKZ74_12975"
     CDS             complement(1673..2401)
                     /gene="recO"
                     /locus_tag="PKZ74_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPM93713.1"
                     /translation="MFQATRGIVFRQTRYSDTSLVARILTEESGLRSYIVKGAHRPKA
                     RIRASIFQPLTLLDMVVSHKEKADLQHIREVRIAYPFQTIPADIRKTSILLFLNELFY
                     KSIQEEASNQELFRFIYDNLILLDQSDENPANFHISMALHLSHFLGFFPQGHYSGFHT
                     VFDLAEGTFTESEPLPAENFISGEVCSWFAKFLTVPVERSYVPEVSAVIRSELLEKIL
                     RYYHLHLPLTGVFKSHKVLHEVLR"
     gene            complement(2401..>2589)
                     /locus_tag="PKZ74_12980"
     CDS             complement(2401..>2589)
                     /locus_tag="PKZ74_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93714.1"
                     /translation="TVKITDIRGSLIYETMAEYNDTQVEWNGLDMNGRRPNTGVFLVF
                     VSNNDGSETMVTKILFVN"
     assembly_gap    2590..2689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2690..3822)
                     /locus_tag="PKZ74_12985"
     CDS             complement(<2690..3822)
                     /locus_tag="PKZ74_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93715.1"
                     /translation="MTYNDTAFFAATESGIFYAAIDDPNLAFYGNWHKIPDLPYPNGY
                     YNLIANYGGRIIVNHADPDITGNNAYVFENGEWNLFEEAEPGRVYYNIRVSGDKLVVS
                     YGYFVIVFNSSLEQELKIYTYGESSPMPSDAMIGTDGYYWLADRSKGLVKVWGGGFNH
                     EFIKPSGAPTADIFDMACSGGKLYLVPGGLTSTWNNSWKGARLYSLIDETWKTYSSSN
                     VPPLDTLRDFVSVAVDPQNSNHVFAGSWNRGMAEFNEGELVVIHNSDNSGLAPNMGLG
                     YPNVKVGGIAFDSENNLWATSSGSEKILAVRRPGGVAAGEWEAYNLGSSTTGAEVKKV
                     IVDSYNQKWIIPRTTQTNPNYIFVFNENNDPGKQFRGLKSGAG"
BASE COUNT          979 a          908 c          966 g          910 t
ORIGIN      
        1 gaacgccgga tcaatgcaac aatgccctta cctttgcaaa gaaattcata aagatggaag
       61 agaacagagg aagaaaaaaa ccggattggc tgaaaattaa ggtccctaaa gggaaagaat
      121 atattggcgt tcgcgatatt attgaccgga acaggcttca cacgatctgt tcatcaggtc
      181 agtgccccaa taaggaagat tgctggggcc gcggaacggc cacatttatg atcctcggga
      241 atatctgcac gcgctcatgc aagttttgtg cggtacccac ggggcgcccg ctgccggccg
      301 atgaagcgga gccggcgcgg attgccgaat cggtacaaaa aatgaagctg aaacactgcg
      361 tcattacatc ggtcgacagg gatgacctgg aagacggagg cgcaaacatc tgggcggcaa
      421 cgatccgggc cgtaaaagag cttaatcccg ccacgacgat cgagacgctg gtgcccgatt
      481 tccgtggccg gaaagagctg ttgcagatag tcatcgatgc ctggccggag gtaatctccc
      541 acaacctgga gacggtaagg aggctgacgc cacaggtacg ttcggcggct atttacgagc
      601 ggagcctcga ggtggtcggc tgggtggccg aatcgggaat ccggtcgaag tcgggcatta
      661 tgctggggct aggagaaacg gaggaagagg tggttcaggt catgaaagat ctccggaggg
      721 tcggttgcga ggtagtgacc atcgggcagt atcttcagcc cactgcggca catttgccgg
      781 ttaaggagta catccggccg gagcagttcg aaaaataccg gcaaacgggg cttgaaatgg
      841 gattccggca tgttgagagc ggcccgctgg tgcgatcgtc ctaccacgcc gaaaaacatg
      901 tgttatgagg gatcgattgt tcaattgttc gattgctaaa tgttaaacta actttgaact
      961 ttgaactttg aactttgaac taaaatatga agatctgggg ctcatccctt accgcaaggc
     1021 ctgggattac caggaaaagc ttttcggcgg gctgatgaaa aagaaactgg ccggtgaaaa
     1081 atcctttccg ggtcacctgt tgttttgtga acatcctccc gtttacacgc ttggaaagag
     1141 cggcaaaaag gaaaaccttc tgttaaatga gcagatgctg gaaaggcagg gaattgaatt
     1201 ttatcacatc aaccggggcg gcgatattac gtttcacggc cccggccaga tcgtcggata
     1261 ccctatcctt gacctggaac aggtttcgat cgggatcagg gaatatatcg aaggactgga
     1321 agaggcggtc atcctggttc ttagccggtt tggaataaag tcggggcggc tttccggcgc
     1381 caccggtgtc tggcttgatc cggagatccc tgccaaagcc agaaagatat gcgccatcgg
     1441 cgtcaaggcc agccggcata tcaccatgca tggatttgct ttcaatgtca ataccgatct
     1501 ttccttttac cgatacatca atccctgtgg ttttatcgac aaaggggtga catctctcgg
     1561 gaaagagatg ggggccatgc agaacataga agaagttaaa ggattgttaa agaattgttt
     1621 agaaagcacc cttgggatat ccatttccga atatcccaat ctcccgtaat aattacctta
     1681 gaacttcatg taatacctta tgcgacttga atacaccggt cagtggcagg tgaagatgat
     1741 aatacctgag gattttttca agcaattcgc tacggattac agcggaaacc tcgggcacat
     1801 aagacctctc gacaggcaca gtcagaaact ttgcaaacca gctgcaaact tccccggaaa
     1861 taaaattctc cgcgggtaat ggttctgact ctgtaaaggt tccttccgca aggtcaaaaa
     1921 cagtatgaaa gcccgagtag tggccctgag gaaagaatcc aagaaaatgt gaaaggtgca
     1981 atgccatcga aatatgaaaa ttggccggat tttcatccga ttggtcaagc agtatcaaat
     2041 tatcataaat aaaccggaac aattcctgat ttgatgcctc ctcctggatc gatttataaa
     2101 acaattcatt caaaaataaa aggatcgatg ttttgcgaat atcggccggg atggtctgga
     2161 aaggatacgc aatgcggacc tcccggatat gttgcaggtc ggctttttcc ttatggctga
     2221 ccaccatgtc gagaagggta agaggttgaa aaatactggc cctgatcctg gctttcggcc
     2281 tgtgggcccc tttgacaatg taggaacgca ggcccgattc ctcggtcagg atccgggcta
     2341 ccaggctggt gtcggagtag cgggtctgcc ggaaaacgat gccgcgcgta gcctggaaca
     2401 ttaattaaca aacaatatct ttgtcaccat cgtttcgcta ccgtcgttat tggagacaaa
     2461 caccaggaaa acgccggtat tgggtcttct tccgttcata tcgagcccgt tccattccac
     2521 ctgtgtatcg ttatattcgg ccatggtttc atagataaga gatccccgga tatcggtgat
     2581 tttaacggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaccggcgcc
     2701 cgatttcagg ccacggaatt gtttccccgg atcgttattt tcattaaaaa caaaaatata
     2761 gttgggattg gtttgagtgg tcctggggat aatccatttc tggttatagg aatccacgat
     2821 cacttttttc acttcggcgc cggtggtgct gcttcccagg ttatatgcct cccattcacc
     2881 tgccgcaacg ccgccaggcc gcctgacagc caaaatcttt tcagagccgg aagaggttgc
     2941 ccataagttg ttctcgctat cgaaagcgat tcccccgact ttcacattcg gatatcccag
     3001 ccccatattc ggagccaggc cgctgttgtc ggagttgtgg atcacaacca gttctccttc
     3061 attaaactca gccattcccc tgttccagga accggcaaac acgtggttgc tgttttgcgg
     3121 atcgactgcg accgagacaa agtccctcag ggtgtcgagt ggcgggacat tggagctgct
     3181 gtaggttttc caggtttcat cgatcagcga atacaatctt gcccctttcc atgaattatt
     3241 ccaggtggag gtcaagccgc cggggaccag gtatagcttt ccgccgctgc aggccatatc
     3301 gaaaatatcg gctgtggggg cgccgctggg tttgataaat tcatggttaa atcctcctcc
     3361 ccatactttc acaagtccct tgctcctgtc ggccaaccag taatatccat ccgtccctat
     3421 catggcatcc gatggcatcg ggctggattc gccgtaagta tatatcttaa gttcctgttc
     3481 caggctggaa ttaaaaacaa tgacaaaata cccgtaagaa acgaccagct tatctccgct
     3541 tacccgaata ttataataga cccggccagg ttcagcctct tcgaacaggt tccattcccc
     3601 gttctcaaaa acataggcat tatttccggt gatatcagga tcggcatgat tgacgatgat
     3661 cctccccccg taattggcga tcaggttgta atacccatta ggataaggta aatccgggat
     3721 tttgtgccag ttaccgtaga aagccaggtt cgggtcgtcg atggcagcat aaaaaatccc
     3781 gctctctgta gcggcaaaga aagcggtatc gttgtatgtc atcccgaaaa cattcaaccg
     3841 gcttccttcc ggcccgatat aat
//
LOCUS       DAOPNH010000303         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_28496_length_3819_cov_4.048618, whole genome shotgun sequence.
ACCESSION   DAOPNH010000303 DAOPNH010000000
VERSION     DAOPNH010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28496_length_3819_cov_4.048618"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKZ74_12990"
     CDS             complement(<1..193)
                     /locus_tag="PKZ74_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPM93716.1"
                     /translation="MAVIKAFRGLRPPVEIVKELASRPYDVLDSEEARIEARGNDYSL
                     LHVIKPEIDLPPEIDHYARE"
     gene            complement(218..682)
                     /locus_tag="PKZ74_12995"
     CDS             complement(218..682)
                     /locus_tag="PKZ74_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPM93717.1"
                     /translation="MDAASNDRNLTFFRFEDLRIYHKALDYVAWIHKASLQFPEAQKD
                     NIGIRYNESARAIALNIAEGSARNKSQFIYYLKMAKSSIRETLVLTSALRKCGYLTDK
                     QETESRGYLMELTKMIGALISSLQRGPAGNHHADDEDDMEFPEHPSFKPESY"
     gene            complement(787..1707)
                     /locus_tag="PKZ74_13000"
     CDS             complement(787..1707)
                     /locus_tag="PKZ74_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPM93718.1"
                     /translation="MKKVLVATDKPFAAIAVKGIRQIVDNAGYELLLLEKYTDPADLL
                     KAVEAADAMIVRSDLVTPEVIIAGKNLKIVVRAGAGFDNIDLPASTAAGVVVMNTPGQ
                     NSNAVAELVFGMLVYQIRNHFNGSSGTELMGKKLGLHAYGNVSKNVARIAKGFGMEVF
                     AFDPFITPDEILKDGVTPVADLKELYSTCQIISLHIPANEKTKKSINFDLLSLMPKNA
                     ILANTARKEVICEDSLQRIFAEREDFRYVSDVAPDCLDSLKEKYSGRFFVTPKKMGAQ
                     TSEANVNAGLAAARQIVEFFEKGDIRFKVN"
     gene            complement(1737..2810)
                     /gene="serC"
                     /locus_tag="PKZ74_13005"
     CDS             complement(1737..2810)
                     /gene="serC"
                     /locus_tag="PKZ74_13005"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPM93719.1"
                     /translation="MKKHNFFAGPSILPAFTVEKTSEAIKDLNGIGLSLMEISHRSKD
                     FQAIIDEAVALFKELLNIPEGYSVIFLGGGASLQFTMVPYNMLETKAAYLNTGEWATK
                     AIKEAKLFGEVVTVASSEDKLFNYIPRGFNVPNDVDYLHVTSNNTIYGTELFEDFNFG
                     VPLVADMSSDILSRPIDVSKYAMIYGGAQKNLGPAGVTFVIVKNDAVGKVTRKIPTML
                     NYKTHIDKGSLYNTPPVVPIFACLQTLKWLKEIGGISAVEKMNISKSDKLYAEIDRNT
                     LFRPTIPNPADRSRMNVCWVMNDNYKHLEEGFQAFATSQGMVGIKGHRSVGGFRASLY
                     NAMPSSSVDALIACMQEFEKQNM"
     gene            2950..>3819
                     /locus_tag="PKZ74_13010"
     CDS             2950..>3819
                     /locus_tag="PKZ74_13010"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPM93720.1"
                     /translation="MIHNEQIIKLISTLGKEFGDAGRVLKENRIGEGNDGSNLVNAAF
                     RAYYENPWFIPEFVGISFISWSKAMTEEKISGWISRYNTGKEKPAKSKRVGIIMAGNI
                     PMVGLHDLICVLASGNQAVIKASSTDQVLIRAVADYLISQEKGIADQIFFTDEPLRDI
                     DAIIATGSNNSFRYFDYYFSKYQHIIRKNRNSIAVITDQDSPEDFEALGDDIFTYFGL
                     GCRNVSKVYHPEKFDINLLINSFNKFSFIKDHHKYRNNYDYQKSVFLVNGISHLDNGF
                     VLARESTSLISPVS"
BASE COUNT         1021 a          869 c          835 g         1094 t
ORIGIN      
        1 cttcccgggc ataatggtct atttcagggg gcagatctat ttcgggcttt atcacatgca
       61 acagggaata atcattcccc cgggcttcga tgcgcgcttc ttccgaatcc agtacatcat
      121 aaggccggga tgccagttct ttaacgattt caaccggggg cctgagtccc cggaaagctt
      181 taattacagc catagttttt agtagttagg ttagtgatca ataagattcc ggtttaaaag
      241 atggatgttc cggaaattcc atatcatctt catcatcagc gtggtgattg ccagccgggc
      301 cccgctgaag ggatgaaatc agggcgccga tcatcttggt cagttccatc aggtaacctc
      361 tggattccgt ctcctgctta tctgtaagat aaccgcattt cctcaatgca gaggtcagta
      421 ccaatgtttc cctgatagaa cttttagcca tcttcaggta gtagataaac tggcttttat
      481 ttctggccga cccttcggca atattcaaag ctatcgcacg ggccgattca ttgtacctga
      541 tcccgatatt gtctttctgt gcctcgggaa actgcagact ggctttgtgg atccaggcca
      601 cgtagtcgag ggccttgtgg taaatcctca ggtcctcaaa cctgaaaaac gtgagatttc
      661 tatcatttga tgcagcatcc ataatggtca atttattggt ttactttcag aataataagc
      721 aggcaggggc tgttccacat tttaaattaa atgctgcatc gaaacagccc ctgatatgtt
      781 tatacactaa tttactttga aacggatgtc acctttttca aagaattcaa cgatctggcg
      841 ggctgctgcc aacccggcat tcacattggc ttccgaggtt tgcgcaccca ttttcttcgg
      901 ggtgacgaaa aatcttccgg aatatttctc tttcaatgaa tccaggcaat ccggcgcaac
      961 gtctgaaaca tacctgaaat cttcgcgttc agcgaagatc ctttgcaggg aatcctcaca
     1021 gattacctct tttctcgctg tattggcgag aatggcattc ttgggcataa gggataacaa
     1081 atcaaaattg atcgattttt ttgttttctc gttagccgga atgtgcagcg aaatgatctg
     1141 acaggtcgaa taaagctctt tcaggtcggc aacaggagta actccatctt ttaaaatttc
     1201 atccggtgta ataaaaggat caaaggcaaa aacctccatt ccaaatcctt tcgcaatacg
     1261 ggcgacattt ttactgacat tgccataggc gtgaaggccc agctttttac ccatcagctc
     1321 agtacctgaa gatccgttaa aatgattccg gatctggtac acaagcatac cgaaaacaag
     1381 ttcggccact gcattggaat tctgcccggg cgtattcatc actacaacgc cagcggcggt
     1441 ggaagcaggt aaatctatat tgtcgaatcc ggcgcctgcc ctgacgacaa ttttcagatt
     1501 ttttccggct ataatcactt ccggtgtaac caggtcgctg cgaacaatca tcgcatcagc
     1561 cgcttcaacg gctttcagca aatcggcagg atcggtatat ttttccagta aaagcagctc
     1621 ataaccagca ttgtcaacta tctggcgaat gcccttcacg gcaatggcag caaaaggctt
     1681 atcggtagca accagtactt ttttcatagt ttcatctatt attttaatct tccgtcttac
     1741 atattctgct tttcaaactc ctgcatacag gcgatcaacg catcgacact cgatgaaggc
     1801 atggcattat agagggaagc cctgaatccg ccaacgcttc tgtgtccttt gattccaacc
     1861 attccctgtg atgtggcaaa agcctggaat ccttcttcaa ggtgtttgta attgtcgttc
     1921 atcacccagc aaacgttcat ccgggaacga tcggccgggt tcggtatggt cggacggaag
     1981 agggtgttgc gatctatctc agcgtaaagt ttgtctgatt tgctgatatt catcttttcc
     2041 acggcactga ttccaccgat ctccttgagc catttcagcg tctggaggca agcgaagata
     2101 ggaacaacag ggggggtatt gtacagcgac cctttatcga tgtgtgtttt ataattcagc
     2161 attgttggga ttttgcgtgt cacttttccc actgcatcgt ttttgacgat cacgaacgtg
     2221 acgccggcag gacccaggtt cttctgggca ccaccataga tcatcgcata tttcgataca
     2281 tcgatcggcc tgctgaggat atccgacgac atatcggcga ccaggggtac accgaaatta
     2341 aaatcttcaa acagctcagt accgtaaata gtattgttgg atgtaacatg gaggtaatcc
     2401 acatcgttcg gcacgttgaa tccgcgggga atataattga agagtttgtc ctcagatgaa
     2461 gccacagtca ccacttcacc gaataatttg gcctctttga ttgccttggt cgcccattcg
     2521 cccgtattca ggtaagcggc cttagtctcc agcatattgt acggaaccat tgtaaactga
     2581 aggctggcac cacctcccag gaaaatcacc gagtaacctt ccggtatgtt gagcaattct
     2641 ttgaaaaggg caacagcttc atcgataata gcctggaaat ctttgctgcg atgtgaaatt
     2701 tccattaacg acaatccaat tccattgaga tctttaatgg cttccgatgt tttttctaca
     2761 gtaaatgccg gaaggataga cggaccggca aaaaagttgt gttttttcat aatagttatc
     2821 gtttttagaa ccttgatttg gtcattgttc attatttaac aaaaagcaaa gatataattt
     2881 ttgtcgtcag cttcaccatc gtgttcttaa ataaataagg gctaaatttg caccgctata
     2941 ttttcattta tgattcataa tgaacagatt ataaaattaa tctccactct cgggaaggaa
     3001 ttcggcgatg ccggtcgggt cctgaaagaa aaccggatcg gggagggtaa cgacgggagt
     3061 aacctggtta atgcggcctt tagggcctat tacgagaacc cttggttcat tcccgaattt
     3121 gtgggtattt cttttatttc gtggtcgaaa gcaatgactg aggagaaaat atccgggtgg
     3181 atcagccgat ataatacagg aaaagagaaa ccagccaaaa gtaagagagt agggataatc
     3241 atggccggga atattccgat ggtgggcctt catgatctga tttgcgtgct ggcctccggg
     3301 aaccaggccg taatcaaagc ttcgtcaact gaccaggttc tgatcagggc agtcgcggat
     3361 tacctgattt cacaagaaaa aggaatagct gaccaaatct tcttcaccga cgaaccgctc
     3421 agggatattg atgccatcat tgcaaccggc agcaataact ctttcaggta ttttgactat
     3481 tacttttcaa aatatcagca tatcatccga aaaaacagga atagtattgc ggttatcaca
     3541 gatcaggatt ctcccgaaga ttttgaagcg ctgggagatg atatctttac ctactttgga
     3601 ctgggttgtc gaaatgtttc gaaagtatat catcccgaaa aatttgatat caacttatta
     3661 atcaacagct tcaacaaatt ttcattcatt aaggatcatc ataagtaccg gaacaattat
     3721 gactaccaga agtctgtatt ccttgtcaat ggtatttctc atctggacaa tggtttcgtg
     3781 ctggcaaggg aatccacttc tttgatttct ccggtatcg
//
LOCUS       DAOPNH010000304         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_28657_length_3803_cov_3.900480, whole genome shotgun sequence.
ACCESSION   DAOPNH010000304 DAOPNH010000000
VERSION     DAOPNH010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28657_length_3803_cov_3.900480"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2180)
                     /locus_tag="PKZ74_13015"
     CDS             complement(96..2180)
                     /locus_tag="PKZ74_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPM93721.1"
                     /translation="MDNPKQNNQNRRELLIKIGLFVFAVVLLVIVLPKEGKFRYEFAK
                     GKPWLHDDLYAPFDFAIIKFDEEIKAEQEQTLKNLRPYFTLDTLVASSQAQLFINTFE
                     NGWKERYGSGNALYQQKEHLINTGLAILDSLFGKGIIELNASIENKPAGFEVYILRKN
                     EAREAPLHKVYTLQQATSFISMKLNRLGGEDTETLRNALVNSITYNLFFDKEKTEAEK
                     NSALESISPFRGMVQRGELIIPRGARVNDDHFRILTSLKNNYEVHLGAAKARYAITGG
                     QAILTSISILILILFLYSYRKDILANNKRIALILLLIFSMVFIASMVVRYEVSYFYLV
                     PVCLVPVIIRVFYDTRLALFVHLITIFITGFIAPNSFQFVFIQLIAGVVAIISIVRLE
                     RRSQFFYTAFWVFLTYVAIYVGLNLMQEGNFHSIHIRHIYQMAGNAGLLLFAYPLMFL
                     IERSFNYITDVTLIELGNSNNKLLRELARKAPGTFQHSLQVANLAEEASFAIGGDSKL
                     VRVGALYHDIGKIEAPLYFIENQVSGINPHDQISHEESARIIIRHVTRGVEIAKKHKL
                     PPEVIDFITTHHGTRKVEYFYIKQKLENPKEGLDEEAFTYPGPIPFSKETALVMMADS
                     VEAASRSLTNPDDKAIDELVERMIGRQLDSGQLAEANLTLRDIAQLKTIFKRNLQNIY
                     HVRISYPEEGGK"
     gene            2245..3024
                     /locus_tag="PKZ74_13020"
     CDS             2245..3024
                     /locus_tag="PKZ74_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPM93722.1"
                     /translation="MIQFGFCELAVIPVRSEPSDKAEMSTQLLFGDIFKVNEIAGPWL
                     FITSFYDGYEGWIDQKQARMISGEEYKRLSSAPLFVNRRIQGDSVKLEGQLVLLPAGC
                     SFYDVKGRVMAVEDQNYVVEGNIFPFRFDNTGSLMETAFSYLSAPYLWGGRTCFGLDC
                     SGFTQIVFKQHGIRLFRDASQQASQGELIPFLSDGMPGDLAFFDNEEGTITHTGILLD
                     NQKIIHCSARVRIDDIDHQGIFNREKMTYTHKLRLIRRVTG"
     gene            complement(3014..3646)
                     /locus_tag="PKZ74_13025"
     CDS             complement(3014..3646)
                     /locus_tag="PKZ74_13025"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPM93723.1"
                     /translation="METPRNVLLPTAYFPPVSYFASLFSSNHALIEACETFPKQTYRN
                     RCEILTSSGRQNLVVPVSKPYGNHTLTRDVLISYREPWHQHHWKSITTAYRSSPYFYY
                     YADLLHPLFNRMESSLLIHNQNILIALIKILGIRPEISLTVDYSKIADGCNDLRTKMK
                     PGRKEPGIFPEYPQVFSHLTGFIPDLSILDLIFNLGPASGDYLNKVVITP"
     gene            complement(3650..>3803)
                     /locus_tag="PKZ74_13030"
     CDS             complement(3650..>3803)
                     /locus_tag="PKZ74_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HPM93724.1"
                     /translation="ELAGVFGIRSIPSLLFCPKDGQPQMAMGALPKDTFRKAIDDILL
                     KAEVKN"
BASE COUNT          933 a          896 c          982 g          992 t
ORIGIN      
        1 cttattaata tctttcggag tgattaaaat ataatataca aatgaaccag aaacctggaa
       61 ccaggaacca ggaacccaaa acccgaaacc cggaactatt tcccgccttc ttccgggtag
      121 ctgatccgga cgtggtagat attctgaaga tttcttttga agatcgtttt tagctgggcg
      181 atgtcgcgca gggtcagatt ggcttcagca agctgccccg aatcgagctg gcggccgatc
      241 atcctttcca ccagctcgtc gatggccttg tcatccgggt tggtcaggct gcgggatgca
      301 gcctccaccg aatcggccat catcaccagg gcggtctcct tggagaaagg gatcgggccc
      361 ggataggtaa aggcctcttc atccaatcct tcttttggat tttccagttt ttgcttaata
      421 tagaaatact ccactttccg ggttccgtga tgagtagtga tgaaatcgat cacctccggc
      481 ggcagtttat gctttttcgc gatctccacg ccgcgggtca catggcggat gatgatccgg
      541 gcactctctt catggctgat ctggtcgtgt ggatttatcc ctgacacctg gttctcaatg
      601 aaatacagcg gcgcctctat tttcccgata tcgtggtaga gggcgccgac ccgcaccagc
      661 ttgctgtcgc cgccgatggc aaacgatgcc tcttcggcca ggttagccac ctggagtgaa
      721 tgctgaaagg tccccggcgc tttccgggcc agttcccgga gaagtttgtt gttgctgttg
      781 cccagttcga tcagcgtgac gtcggtaata taattgaacg accgctcgat caggaacatc
      841 agcgggtaag caaatagcag caatccggca ttgcctgcca tctggtaaat atgccggatg
      901 tgaatgctat ggaagttgcc ctcctgcatc aggttaagcc ctacataaat cgccacataa
      961 gtcaggaaca cccagaatgc cgtgtaaaaa aactggctcc gcctttccag cctgacaatg
     1021 ctgatgatcg ccaccacccc ggctatcaac tggataaata cgaactggaa actgttcggg
     1081 gcaatgaagc cggtgataaa gatggtgatg aggtggacga aaagcgccag gcgtgtgtcg
     1141 taaaacaccc ggataatcac cggtaccagg cataccggta ccaggtagaa gtagctcact
     1201 tcatacctga cgaccatgct ggcgataaat accatggaaa agataagcaa caggatgagg
     1261 gcaatacgct tgttattagc caggatatct tttctgtaac tatagagaaa caggattagg
     1321 atcagaatgg aaatggaagt caggatagct tgtccgcccg taatggcgta ccgggctttg
     1381 gcagccccca ggtgtacctc gtaattgttt ttgagcgagg tcaggatgcg gaagtgatcg
     1441 tcgttgaccc gcgccccccg gggaatgatc aattcgcccc gttggaccat gcctctgaac
     1501 ggactgatgc tttcgagcgc tgagtttttc tcagcctcgg tcttctcctt gtcgaagaac
     1561 aggttgtagg taatggaatt gaccagggca ttccggagcg tctctgtgtc ctcgccgccg
     1621 aggcggttta atttcatgga aatgaaactg gtggcttgct gtaaagtgta taccttatgc
     1681 aaaggtgctt cccgggcttc gttctttctc aggatgtaaa cctcgaaacc ggccggtttg
     1741 ttttcaatcg aagcattcag ttcgataatg cccttgccga ataacgaatc caggatcgct
     1801 aacccggtgt taatcaggtg ttctttctgc tgatacaagg catttccgct cccatacctc
     1861 tctttccatc cgttttcaaa cgtattgatg aacaattggg cctgggagga agccacaagc
     1921 gtgtcgaggg taaaatatgg cctgagattc tttaatgtct gctcctgttc cgctttgatc
     1981 tcttcgtcga acttgataat ggcaaaatca aaaggcgcat acaaatcgtc gtgcagccag
     2041 ggtttccctt tggcaaactc gtagcggaac tttccctcct tgggtagtac gatcaccagc
     2101 aataccacgg caaaaacgaa taaaccgatt ttgatcagta actcgcggcg gttttgatta
     2161 ttttgctttg gattgtccat gatgcttact tcctgctgct aatttagaga ttttaggttt
     2221 aaatttgcac gaaatcatat ttttatgata caattcggtt tttgtgaact cgccgtaatt
     2281 cctgtcaggt ccgaaccttc tgataaagcg gaaatgtcga cacagctgct gtttggcgat
     2341 atatttaaag tgaatgaaat agcgggtccc tggcttttta ttaccagttt ttatgacggt
     2401 tacgaaggat ggatcgacca gaaacaggcc cggatgatca gcggggaaga atataaaagg
     2461 ctatcctcag ccccattatt tgtaaacagg agaattcagg gtgattcggt aaaattggaa
     2521 ggacagcttg ttttattgcc ggccggatgt agtttttatg atgttaaagg acgggtgatg
     2581 gccgtcgaag accagaatta tgtcgtggaa ggaaatatat tccctttccg gtttgacaac
     2641 actggcagct taatggaaac ggccttcagc tatctatcgg cgccttatct ctggggaggc
     2701 aggacttgtt ttggcctcga ttgttcgggt tttacccaga tcgtctttaa acagcacggc
     2761 atccggcttt tccgggatgc ttctcagcag gcatcccagg gcgaactcat tccctttctg
     2821 agcgacggca tgccgggcga tctcgccttt ttcgacaacg aagagggcac tatcactcac
     2881 acgggcatcc tgctcgataa ccagaagatc attcattgta gcgccagggt aaggatcgat
     2941 gatatcgatc accagggtat cttcaaccgt gaaaaaatga cctacacgca caagctgagg
     3001 ctgatcaggc gggtcacggg gtaataacca ctttgtttaa ataatctccg gaagcagggc
     3061 cgagattgaa aatcaggtcc agtatgctca ggtcagggat aaatcctgtc agatgactga
     3121 aaacctgggg gtattcggga aagattccgg gttctttcct gcccggcttc attttggtcc
     3181 gaagatcatt gcaaccatct gctatcttag agtaatcaac ggtcaggctt atctccggtc
     3241 ttatccccag tattttaatt aaagcaataa gaatattttg attatggata agaagcgagc
     3301 tctccatccg gttgaagagc gggtgcagca ggtcggcgta gtagtaaaaa tagggcgatg
     3361 agcggtatgc ggtagtaatg gatttccagt gatgctggtg ccagggttcc cggtaagaga
     3421 tcaaaacatc ccgggttaat gtatgatttc cgtaaggctt tgataccgga actaccaggt
     3481 tctgcctgcc cgaggaggtc aggatctcac atcggttccg gtaggtttgc tttggaaagg
     3541 tttcacaggc ctcgatcaga gcatggttgc tgctgaaaag gctggcaaaa taagagaccg
     3601 gcgggaaata cgctgtagga agaagcacat ttctcggggt ttccacagct taatttttta
     3661 cttccgcttt caggagaatg tcgtcgatgg ctttccggaa ggtgtctttc ggcagggcgc
     3721 ccatggccat ctgcggctgg ccgtctttcg ggcagaacaa cagcgaaggg atactgcgga
     3781 taccgaaaac accggccagt tcc
//
LOCUS       DAOPNH010000305         3747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29252_length_3747_cov_6.620531, whole genome shotgun sequence.
ACCESSION   DAOPNH010000305 DAOPNH010000000
VERSION     DAOPNH010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29252_length_3747_cov_6.620531"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PKZ74_13035"
     CDS             <1..1183
                     /locus_tag="PKZ74_13035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM93725.1"
                     /translation="EIKIPNIPYYGMLDDGIGYIKLTNFTQNAGGEVKKAFNELKTNN
                     ELKGLIIDLRGNGGGLLNEAVNISNIFVDQGQLIVSTKGKIAAKTQDHFTRSAAIDKE
                     IPLAVLVDKNSASASEILAGAMQDLDRGVVIGQRTFGKGLVQNVIPLTYNSQVKITVA
                     KYYIPSGRCIQSIDYFHKDENGKSDKIPDSLISEFKTKNGRPVYDGGGIEPDIKLPAL
                     KLSKISQALVTKYLIFDFANQYASKHATIPVAGEFEVTDEIYTEFTAFLQDKDYDYKT
                     ASEEALEKLKSNAVREKYFDSIEEEYNALKGKILHDKSQDLITNKKEIKMLLKDEIVA
                     RYYFQTGRVVASLNDDPEIEKAIEVLTGRDTYLAILDGSLKPDQKEKNTETDNGSDEE
                     E"
     gene            1170..1661
                     /locus_tag="PKZ74_13040"
     CDS             1170..1661
                     /locus_tag="PKZ74_13040"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPM93726.1"
                     /translation="MKKSEIRIIVEDYLSAEELPVQDRQLLAEAITGASTSYSPYSSF
                     RVGAAVRLDNGEIITASNQENAAYPSGLCAERVALFYAQANYPESSVTGIAIYAESAE
                     FTIERPVTPCGSCRQVMAEHEYRHGQKMRIIMGSSKGNVSVVEGIESLLPMQFVMEGL
                     RRE"
     gene            1865..2734
                     /locus_tag="PKZ74_13045"
     CDS             1865..2734
                     /locus_tag="PKZ74_13045"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPM93727.1"
                     /translation="MKRGSVVLGVLFMLTVAMKSDKPAYIIFDVKGKQADYKDLLKSA
                     ADADIVFFGEMHDSPICHWLQYELTNDLFKEVNEDLILGAEMFETDNQLILDEYLAGK
                     IKDKNFEDEAKLWDNYKTDYKPLVKYALDKKLRFIATNVPRRYASLVNKSGFEGLDSL
                     TTEANAYLPPLPITYDPELEGYKSMMEMMEGAGGHASPNLPKAQALKDATMAHFILKN
                     IKKGDIFLHFNGTYHSNNFEGIVWYIKKERPDLKIITIASVEQDSPEELTEENSGLAD
                     FTICIPSTMSKSY"
     gene            2738..3493
                     /locus_tag="PKZ74_13050"
     CDS             2738..3493
                     /locus_tag="PKZ74_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPM93728.1"
                     /translation="MLSKNQVKYIQSLRLGKFRDEYRVFIVEGVKMVDELLKSSFHIR
                     QILGTTSWIETHKDILKAKNPEVQVITEAELQRISHLVTPNEVLAVAGYPENDLPARE
                     GLGKIILVLDGIQDPGNLGTIIRTADWFGIRQVICSENTADIFNPKVVQATMGSIFRA
                     GIYYTVLKDFLEGLGPDWKIYGADTEGENIFGLEPGFPLAIVIGNESKGISPGLYPLL
                     TQKIGIPSGSTGAESLNAAVAAGIIMAVFGRKI"
BASE COUNT         1112 a          802 c          953 g          880 t
ORIGIN      
        1 agagataaag ataccgaata ttccttatta cgggatgctc gacgacggca tcggttatat
       61 taaactgacc aattttaccc agaatgcggg gggtgaggtc aaaaaggcct ttaacgagct
      121 gaaaaccaat aatgagctta aaggcctgat catcgacctg aggggcaatg ggggcggttt
      181 attgaacgaa gctgtcaata tcagcaacat ttttgtcgac caggggcaat tgatcgtcag
      241 cacaaaaggt aaaattgcag ccaagaccca ggatcatttt acccggtcgg ctgccatcga
      301 taaagaaatc ccgctggcgg tgctggtaga caaaaacagc gcatccgctt cagagatcct
      361 cgccggcgcc atgcaggacc tggaccgggg agtggtcatc ggtcagcgca cctttggcaa
      421 aggattggta cagaacgtta taccgctcac ctacaattcg caggttaaaa ttaccgtagc
      481 caaatattat attcccagcg gccgttgcat tcaatcgatc gattatttcc ataaagatga
      541 aaacgggaag tcggataaaa ttcctgactc actgatttcc gagttcaaaa caaagaatgg
      601 aaggcctgta tatgacggcg gcggcatcga acccgatatt aaattgcctg cactaaagct
      661 cagcaaaata tcacaggccc tggtcacaaa atacctgatt tttgattttg caaatcaata
      721 tgcttcaaaa catgcaacga tcccggttgc cggcgaattc gaagtaactg acgaaatata
      781 taccgaattc acggcttttc ttcaggataa ggattacgac tataagaccg ccagcgaaga
      841 agccctggaa aaactgaagt caaatgcggt cagggaaaaa tacttcgatt caatcgaaga
      901 agagtacaat gctcttaaag gcaagatcct gcacgacaaa tcccaggatt tgatcacgaa
      961 taagaaggag atcaaaatgt tgctgaaaga tgagattgtt gcccgttatt attttcaaac
     1021 cggccgggta gttgcttctc tcaacgacga tccggaaatt gaaaaagcca ttgaagtatt
     1081 gaccggaagg gacacctacc tggcgatcct cgacggaagt ttgaagccgg atcagaagga
     1141 aaaaaacaca gaaaccgaca acggcagcga tgaagaagag tgagatcagg atcatcgtgg
     1201 aagattacct gtcggctgaa gagttgcctg ttcaagacag acaattgctg gcagaggcca
     1261 taacaggagc ttccacctct tactccccgt attcttcatt cagggtcgga gctgccgtaa
     1321 gactggataa cggtgagatt atcacagcca gcaaccagga gaatgcagct tatccttcag
     1381 ggctttgcgc cgaacgtgtc gccctcttct atgcccaggc caattaccct gaatccagtg
     1441 tgaccggtat cgctatttat gccgaatcag ctgagttcac cattgaacgg ccggttacac
     1501 cctgtggatc atgccggcag gtcatggccg aacacgagta ccgccatggt cagaaaatgc
     1561 ggatcatcat gggaagcagc aagggaaacg tcagcgtagt tgaaggcatc gagtccctgt
     1621 tgccgatgca gttcgtgatg gaagggttgc gcagggaata gtgaacagtg aacagtgaac
     1681 ggtgaacagg gaacagtgag cagtgaacgg tgaacggtga acagtgaaca gggaacagtg
     1741 aacagtgaac ggtgaacagt gaacagtgaa cagtgaacag tgaacagtga acagtgaacg
     1801 gtgaacagtg aacagggaca gggaactggg aactggggaa agttaatgat aatattaggt
     1861 taatatgaaa agaggaagtg tagtacttgg agttttgttt atgctgacgg tggcgatgaa
     1921 atcggataaa ccggcgtata ttatttttga tgttaaaggg aaacaggctg attacaagga
     1981 tttattgaag agtgccgcag atgccgatat tgtatttttc ggtgagatgc acgattcccc
     2041 gatatgccat tggttgcaat atgaactcac aaatgattta ttcaaagagg tcaatgaaga
     2101 tctgatcctg ggggctgaaa tgtttgagac cgacaatcag ctgatcctcg atgaatacct
     2161 ggccggaaaa ataaaggata agaattttga ggatgaagcc aaactctggg ataattacaa
     2221 gaccgattac aagcccctgg tcaagtatgc cctggataaa aagctccgtt ttatagccac
     2281 gaatgtcccc cggcgctatg cttcgctggt caacaaatcg ggttttgaag ggcttgacag
     2341 tctgacaaca gaagctaatg cgtatcttcc tccccttcca ataacttatg atccagaact
     2401 ggaagggtat aaaagcatga tggagatgat ggaaggagcc ggcgggcatg cttcaccgaa
     2461 cctgcccaaa gcccaggcgc tgaaagatgc gaccatggct cattttatcc tgaagaacat
     2521 taaaaaagga gatattttcc tgcattttaa cgggacttat cattccaata acttcgaagg
     2581 cattgtctgg tatataaaga aagaacgtcc tgacctgaaa atcattacca tagcctcagt
     2641 ggagcaggat tcgccggagg agttgacaga agaaaactct gggcttgcag attttactat
     2701 ctgcatccct tcgacgatga gtaaatccta ctagaagatg ctatccaaaa accaggtaaa
     2761 atatatccaa tcccttcgcc tggggaagtt cagggatgaa taccgggttt ttattgtgga
     2821 aggggtaaaa atggtcgatg agctattgaa aagctctttt catatccgcc agattctcgg
     2881 gacaacctcc tggatcgaaa cccataaaga tattctaaaa gcaaaaaatc ctgaagtcca
     2941 ggttatcact gaggctgagc tgcagaggat cagccaccta gtgacgccga atgaagttct
     3001 tgccgtggcc ggttatcctg agaatgacct tcccgccagg gaaggcctgg gaaaaattat
     3061 tctggtcctc gacggcatcc aggatccggg gaacctggga acgataatcc ggacggccga
     3121 ctggttcggg atcaggcaag tcatatgttc tgaaaatacg gcggatattt tcaatcccaa
     3181 ggtcgtgcag gctacgatgg gatcgatatt ccgggcaggc atttactata ccgtcctgaa
     3241 agatttcctg gaaggccttg gccccgattg gaagatttac ggggccgata cggagggcga
     3301 gaacattttc ggccttgagc ccggttttcc tttggccatc gtaatcggca atgaatcgaa
     3361 agggatctct cccgggctat atcctttgtt aacgcagaaa ataggtattc cctccggttc
     3421 taccggcgcc gaatcgctga atgcggctgt tgcggcaggg atcatcatgg cggtgtttgg
     3481 caggaaaatt taagtagtaa tgggcaggaa gttctttgtt agcaataaaa tgcccgattc
     3541 ggcattcggc attgcgcatt ttcgatttaa tgtggtagtc cgacgggtaa gtccgtcggt
     3601 tccaggataa tatctttcct atcggaagaa aaatccacat tgaactgctt gttgagtagg
     3661 gtatttctgt gagactccat ctcaattcat ttgagatgcc atctcatgca aaccagatgg
     3721 agtctcaaac caaaaagtta tcaaacg
//
LOCUS       DAOPNH010000306         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29460_length_3730_cov_5.421224, whole genome shotgun sequence.
ACCESSION   DAOPNH010000306 DAOPNH010000000
VERSION     DAOPNH010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29460_length_3730_cov_5.421224"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..650
                     /locus_tag="PKZ74_13055"
     CDS             135..650
                     /locus_tag="PKZ74_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93729.1"
                     /translation="MYRISITGPESTGKSALAKELAAHFGTVWVPEYAREYLEKLGRR
                     YEFEDIAIIAIGQLEQENRMAAKADQIVFCDTDMLVCKIWSEFRYGHCDAWLQKIVRS
                     HVYDLYLLCDIDLPWEEDPLRENPKERQELFDIYLRELQKMNANFGIVTGTGDQRLRN
                     AISAVVGRLSW"
     gene            785..1963
                     /locus_tag="PKZ74_13060"
     CDS             785..1963
                     /locus_tag="PKZ74_13060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM93730.1"
                     /translation="MSSPDKNKVLFMARWYPNRYDPMPGLFIERHARSVSEHYDMAVL
                     YIHQDEELKNTAFEIEKFRDKELFQVKLYYRPVQLRIPAIGRFINFLLYIFYSFKGLL
                     LLEKEWGQPDLIHVNVLTRIGAIALIHKWLTRVPYVITEHWTRYLPHMDNFRGRIRKA
                     VTRLVVKEASAVLPVTDNLRMAMESHGLTNKNYRIIPNVVDMNMFTVMDWTMTGKKHF
                     IHVSCFEDRQKNISGILRVLKRLSETRDDWECVMIGEGIHLERLIDYAKELEIEGKFV
                     RFTGLKENGELAELMAKTGFQVMFSRFENLPVVILESYACGVPVLSTDVGGIKEHLTD
                     DLGILIRSEDEEELFLKLNEMLDRYGSFDKNKIRDYAERHFSREVIGRQLAEIYREAI
                     "
     gene            1976..3262
                     /locus_tag="PKZ74_13065"
     CDS             1976..3262
                     /locus_tag="PKZ74_13065"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPM93731.1"
                     /translation="MFGKIINTFGTRALAAVINLGIAIALSQYLGPAGKGVQSLIITT
                     ITFVLVFANLIGGATLVYLVPRREPSLLLLPSIVWTLVVSALSFAILQVFPLVDKTYS
                     AHICILSAINSFTSITTSILIGREKIRAANAVGLVQPAVLILSLFLFFGNSPSPGVDH
                     YIWSLYFSFIFSAIAGMIYYQRYCGKIRFFGMAEYLKVSLEMARYGVLNQVAHVTQML
                     SFRLSFYVLDRYHGEAAVGIYSNGISIAESIWLIAKSISLVQYARISNLNNREESAKL
                     TVSLMKFSLAASLVLILPVLLLPESFYLWVFGEGFGEMRYVIQALAGGVLVYNFSILA
                     GHYFSGTGRYQVNAITSTLGLIASIILYYSMIPSLGITGAGFATSLSYLFTSLILMYL
                     FGREYKTWYREIIPVKGDLIRLRDGLKEMMNKKPGS"
     gene            complement(3259..>3730)
                     /locus_tag="PKZ74_13070"
     CDS             complement(3259..>3730)
                     /locus_tag="PKZ74_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPM93732.1"
                     /translation="NNWPPQYTAEFDLFYGPKGSNLVRIDLPYSMKLAWDPAVTVNSF
                     SCHAKVHDSMLRVLTAVLNHYGIAEIRRLKLDLFGGCYNQRQIRGGTKWSMHSWGIAV
                     DFDPARNKLEWGRDKAAFAGQEYDKWWELWETEGWVSLGRQRNFDWMHVQAAKL"
BASE COUNT          928 a          841 c          965 g          996 t
ORIGIN      
        1 ccagtaaatc gctggtattg agcagctttc aatttgccgt attcctggcg ctggccattg
       61 ccggatatat tgaatggagg aagaaattta aactcaaaac tacttcaatt taacactaag
      121 ttttagtcct gattttgtac cgtatttcga tcaccggccc ggaatccacc gggaagtcgg
      181 ccctggcgaa ggagcttgcc gcacatttcg gtacggtctg ggttcccgaa tatgcccgcg
      241 aatatcttga gaagcttggc aggaggtatg agtttgagga tattgcaatt attgccatag
      301 ggcagcttga acaagaaaat agaatggcgg ccaaagccga tcaaattgta ttttgtgata
      361 ccgatatgct ggtttgcaaa atctggagtg aattcaggta cggacattgc gacgcctggc
      421 ttcagaagat cgtaagaagc catgtctacg acctgtacct tctgtgtgac atcgatcttc
      481 cctgggaaga agatcctttg cgggaaaatc caaaggaaag gcaggagtta tttgatatct
      541 atctccgtga gcttcagaaa atgaatgcta actttggcat cgtgaccgga accggggatc
      601 aacgtcttcg gaatgcaatt tcagcggttg tcggacgttt gtcctggtag gcagccggca
      661 gtcggcagtc ggcagtaagc agtcggcagt cggcagtcat aagttcatta gatcagaata
      721 tcaaaatatc acaagttcaa aatatcataa tatcaaaata tcaaaaatca acccaagaaa
      781 cggcatgtct tctccggata aaaacaaggt tctttttatg gcccgctggt atccaaaccg
      841 ttacgatccg atgcccggtt tattcattga gcggcatgcc cggtcggtgt cggagcatta
      901 cgacatggct gtgctatata ttcatcagga tgaagaatta aaaaacactg catttgagat
      961 tgagaaattc agggataaag agcttttcca ggtaaagctt tattaccggc cggtacagtt
     1021 aaggattcct gcaatcgggc ggttcatcaa tttcctgctt tatatttttt actccttcaa
     1081 agggttatta cttttagaaa aggaatgggg ccagcccgat ctgatccatg ttaacgtatt
     1141 gacccgcatt ggagccatag cactcattca caaatggtta accagggttc catatgtgat
     1201 caccgagcac tggacccggt atttaccgca tatggacaat ttcaggggaa ggatcaggaa
     1261 agccgtcaca cggctcgtgg tgaaagaagc ttctgctgtt ctcccggtaa ccgacaattt
     1321 gcggatggct atggagagcc acgggctgac caataaaaac taccgcataa tacccaatgt
     1381 ggtggatatg aatatgttca cggtaatgga ttggacaatg accggcaaaa agcactttat
     1441 ccatgtttcc tgctttgaag accgccagaa gaatatatcg gggatactgc gcgtgttgaa
     1501 gagattgtcg gaaacaaggg atgattggga atgtgtgatg atcggcgaag ggatccatct
     1561 ggaaaggctt attgattacg caaaagagct tgagattgaa ggaaagttcg tccggtttac
     1621 cggcttgaaa gagaacggtg aactggcgga attaatggca aaaacaggat tccaggtgat
     1681 gttcagccgg tttgagaacc tgccggtggt tatcctggaa agctatgcct gtggcgtgcc
     1741 ggttttgtcg acggatgtgg gcggaataaa agaacatttg actgacgacc tgggaatttt
     1801 gatcaggagt gaagacgaag aagagctttt cctgaagctg aatgagatgc ttgaccggta
     1861 cggatctttc gataaaaaca agatcaggga ctatgccgag cggcatttta gccgggaagt
     1921 tatcgggcgg caactggcag agatatatcg ggaggcgatt taacaaccaa cgaccttgtt
     1981 tggtaaaatc ataaatacat tcggaacgcg ggcgcttgcg gctgtgatca acctggggat
     2041 tgcgattgca ttgtcgcagt atctcgggcc ggcaggcaag ggggtacaga gcctgatcat
     2101 aaccacgatc acttttgtgc tggtatttgc caaccttatc ggcggggcga cactggtata
     2161 ccttgttcca aggcgcgaac cctcgctcct gctactgcct tccatcgtat ggacattggt
     2221 cgtaagcgcc ctgtcgtttg cgatattgca ggttttcccg ctggttgaca aaacctactc
     2281 ggctcatatc tgtatactgt ctgcgataaa ttccttcact tccatcacaa cctccattct
     2341 gatcggcagg gaaaagatca gggcagccaa tgcagtcggt ctggttcagc cggccgtact
     2401 gatcctttca ttgtttctgt ttttcggaaa ttctccttcc ccgggagttg atcattacat
     2461 ttggtcgctc tatttttcct ttattttttc ggcaattgca gggatgattt attaccaaag
     2521 gtattgcgga aagatcaggt tttttggtat ggcagaatac cttaaagtca gcctggagat
     2581 ggcccgctac ggagttctga accaggtggc ccatgtgaca cagatgctca gtttcaggtt
     2641 gagcttttat gtgctcgacc ggtatcatgg cgaggcggct gtcgggattt attctaacgg
     2701 gatctccata gccgaatcca tctggctgat tgccaaaagc atatcgcttg tacagtatgc
     2761 ccgtatttcc aatttgaata acagggaaga atcggcaaag ctgacggtca gtctgatgaa
     2821 attcagcctg gcagccagcc tggtattgat cctccccgta ttattgttac ctgaatcatt
     2881 ttacctgtgg gtattcggtg agggattcgg ggagatgcgt tacgttatcc aggccctggc
     2941 aggcggtgtg ctcgtctata atttttccat cctggccggg cactattttt cagggacagg
     3001 ccggtaccag gtcaatgcca ttacctcgac actcgggttg attgcttcga taatccttta
     3061 ttattcgatg atcccatcgc tgggtataac aggagccggc tttgccacct ccctgtcata
     3121 tctctttacc agcctgatcc tgatgtattt gttcggtcgc gaatataaaa catggtacag
     3181 ggagatcatt cctgtgaaag gtgatcttat ccgtttgcgt gacgggttga aagagatgat
     3241 gaacaagaaa cccggaagct agagtttggc tgcctgcaca tgcatccagt cgaagttgcg
     3301 ctgccggccc aggctcaccc acccttccgt ttcccagagt tcccaccatt tgtcgtattc
     3361 ctgcccggcg aaggcagctt tatctctccc ccattcgagc ttgttccggg ccggatcgaa
     3421 gtcgaccgca atcccccagc tgtgcatcga ccattttgtt ccgcccctga tctggcgctg
     3481 gttatagcat ccgccgaaca ggtcgagttt gagccgccgg atttcggcga ttccgtagtg
     3541 gttcagcacg gcagtcaaca ccctgagcat actgtcgtgc acttttgcgt ggcaggagaa
     3601 tgaattaacc gttacagcgg gatcccaggc cagtttcatg ctgtaaggaa ggtctatcct
     3661 gacaaggttg gagcctttgg ggccgtagaa caaatcgaac tcagcagtgt actggggtgg
     3721 ccagttattg
//
LOCUS       DAOPNH010000307         3718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29597_length_3718_cov_6.538084, whole genome shotgun sequence.
ACCESSION   DAOPNH010000307 DAOPNH010000000
VERSION     DAOPNH010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29597_length_3718_cov_6.538084"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PKZ74_13075"
     CDS             <1..640
                     /locus_tag="PKZ74_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPM93733.1"
                     /translation="TILITNEKALSIIQEKLAIYDKSGEMHYDVISAFIKSLRGSDPN
                     GAVYWLARMIEGGEDPLFIARRMLILACEDVGLANPNALLLANACFDAVHKIGMPESR
                     ILLSECAVYLATSQKSNASYMAIEEALEEVRRSGDLPVPLHIRNAPTKLMKEIGYAKD
                     YKYAHSYEGNFVMMEFLPEKIKGKKFYSPGKNKREEEISLWLRSRWKEKYNY"
     gene            714..1526
                     /gene="kdsA"
                     /locus_tag="PKZ74_13080"
     CDS             714..1526
                     /gene="kdsA"
                     /locus_tag="PKZ74_13080"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPM93734.1"
                     /translation="MIDKLTKISHTSSGNFFLIAGPCVVEDEKMPFEIAEAVLKICDR
                     LNIPYIFKASYKKANRSRLYSFTGIGDEKALGIIRNVGEQFGIPVLTDIHNEQEAVLA
                     AAYADVLQIPAFLCRQTELLVAAAKTGKFVNVKKGQFVSPEMMKFVVEKVQQSGNPNV
                     ILTERGSTFGYQDLVVDFRAIPVMKSFGVPVVLDVTHSLQVPNRASGTSGGQPQFIET
                     LAKAGIAAGVDGLFLETHPDPGCAKSDGENMLRLDLLDNLLTKLITLKKALL"
     gene            1717..2067
                     /locus_tag="PKZ74_13085"
     CDS             1717..2067
                     /locus_tag="PKZ74_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPM93735.1"
                     /translation="MSAESLILFFILGAFLVGIALLFSSYIPPRSTNPQKAEPYECGI
                     PTTGLSWLQFNIGYYLFAILFLIFEVETVFLFPWAVVMKELGMVAFIEILIFLVILGL
                     GLLYAWNKKALVWE"
     gene            2073..2630
                     /locus_tag="PKZ74_13090"
     CDS             2073..2630
                     /locus_tag="PKZ74_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPM93736.1"
                     /translation="MKETDFHVSPDTDRQKTNIVLTTVENLVNWGRSNSVWPLTFATS
                     CCGIEMMATGASRHDFARFGVEVYRATPRQADVIIIAGTIVHKMAPVLKRLYDQMAEP
                     KYVIAMGACAISGGPFFYNTYSVIRGADQVIPVDVYIPGCPPRPEALLYGIMQLQRKI
                     QQGNIAAGRIEPVDPEEYPEPPKVY"
     gene            2653..3114
                     /locus_tag="PKZ74_13095"
     CDS             2653..3114
                     /locus_tag="PKZ74_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPM93737.1"
                     /translation="MDNTNIKEFVARHIPEATFEENKQFLSATVEPSKLHGLLKALKE
                     NPETLFDYLFCITGADVQGKLMVIYHLESTTLKHQVVVKTGTPDRENPVFDSITDLFS
                     GAELHENEIFDLFGIIFKGHPNLRRMFMPDDWKGYPLRKDYTDEVNIVELS"
     gene            3125..>3718
                     /locus_tag="PKZ74_13100"
     CDS             3125..>3718
                     /locus_tag="PKZ74_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPM93738.1"
                     /translation="MEENTKPMTAPETEEEYFINMGPQHPSTHGVLRLLVSLQGEIVK
                     RLDPHLGYIHRGIEKMCEKDTYIQIIHLTDRMDYLSAHMNNEAVCLLVEDALEIEVPE
                     RVKYIRTIMAELQRLNSHQLWWATMGMDLGALTTFFYGLRDREKILDIFEETCGGRLL
                     LSYNTPGGVMYDIHPNFQKRVKEFIKYFRKKIRDYDDI"
BASE COUNT          989 a          906 c          905 g          918 t
ORIGIN      
        1 taccatcctc attaccaatg aaaaagcctt aagcattatc caggagaagc tggccattta
       61 tgataaatca ggtgagatgc attacgatgt catttcggct tttatcaaat cgctgagagg
      121 cagcgatccg aacggggcag tctactggct ggcccggatg atcgagggcg gcgaagatcc
      181 gctgttcatt gcgcgtagga tgctgatcct ggcctgtgaa gacgtcggcc ttgccaatcc
      241 gaacgcattg ttgctggcaa atgcctgttt cgatgcagtg cacaagatcg gcatgcccga
      301 aagccgcatc ctcctgtcgg aatgtgcggt atacctggcc acatcacaaa aaagcaacgc
      361 ctcctacatg gcgatcgaag aggcacttga agaggtcaga agatcgggcg acctgcctgt
      421 tccgcttcat atccggaatg ccccgacaaa gctgatgaaa gagatcggtt acgcaaaaga
      481 ttacaaatat gcacactcct acgagggaaa tttcgttatg atggagttcc tgcccgaaaa
      541 gatcaaaggg aaaaagtttt actcacccgg gaaaaacaag cgggaagaag agatcagcct
      601 ctggctcagg agcaggtgga aggagaaata caattattag ttccaggttc caggttccag
      661 gttcattccg ccagttggcg gaccaagttt caaatttcaa agttctaatg tcaatgatcg
      721 ataaactaac caaaatcagt cacacttctt ccgggaattt ctttctgatc gccggacctt
      781 gtgtggtgga agatgaaaaa atgccgtttg aaattgctga agcggttttg aaaatatgtg
      841 accggttgaa tataccttat atttttaagg cgtcctacaa aaaagccaac cgatccaggc
      901 tctattcttt caccggcatc ggagatgaga aagcactcgg gatcatccgg aacgtcggtg
      961 agcagttcgg catcccggtc ctgaccgata tccacaacga acaggaagcg gtgctggcag
     1021 cggcctatgc cgatgtgctt cagatccccg cctttctttg ccggcaaacg gagttgcttg
     1081 tcgcggcagc taaaacaggg aaattcgtga atgttaaaaa aggacagttt gtatcgccgg
     1141 agatgatgaa attcgttgtc gaaaaggtgc agcaatcggg caatccgaat gttatcctga
     1201 ccgaacgggg aagcacattc ggttaccagg acctggttgt ggatttcagg gccattcctg
     1261 taatgaaatc tttcggcgtc ccggttgtgc tcgatgtgac ccactccctg caggtaccca
     1321 accgtgcatc cggtacctcc ggcggccagc cccaattcat cgaaacactc gccaaggccg
     1381 gtattgccgc cggagtggat ggactgttcc tggaaacgca cccggatccg ggatgtgcta
     1441 aatctgacgg ggaaaatatg ctgagactcg acctgcttga caatctcctc acaaaactca
     1501 tcactttaaa aaaagccctc ctctaaggga agtttttaac attacttaat ttagaatcaa
     1561 tttatattat tgtatatgat tgtaattcaa taatataaga tgatgtatta atcatccgct
     1621 gtttattaac atatcagata tatcgtttta tgaatattta aaaataaaag attttagatt
     1681 tgcggaaatt tttgagttgt ttaaaacaat caaagtatga gcgcagaatc tctaatcctg
     1741 tttttcatcc tcggggcctt cctggtggga atcgcattgc ttttttcatc ctacatcccg
     1801 ccccggtcga ccaatcccca gaaagcagaa ccctatgagt gcggtatccc gactacgggt
     1861 ttatcctggc ttcagttcaa catcgggtac taccttttcg ccatcctttt cctgatcttc
     1921 gaagtggaaa cggtattcct gtttccatgg gcagttgtca tgaaagaact gggaatggtc
     1981 gcttttattg aaattttaat tttcctggtc atcctgggtc ttggtctttt atatgcatgg
     2041 aataaaaaag cattggtatg ggaataaaat cgatgaaaga gactgatttt cacgtctctc
     2101 ccgacacaga tcgtcaaaaa acaaatattg tactgactac tgttgagaac ctggtcaact
     2161 ggggccgttc caattcggtg tggcctctga catttgccac cagttgctgc ggtatagaga
     2221 tgatggccac gggtgcatcg cgacacgatt ttgcccggtt cggagtagaa gtttaccgtg
     2281 ccaccccccg tcaggccgac gtgatcatca tcgccggcac gatcgtgcat aagatggcgc
     2341 cggtactgaa gcggttgtac gaccagatgg ccgagcccaa gtatgttatc gccatgggcg
     2401 catgcgccat ttcgggcggg ccgttctttt acaatactta ttcggtgatc cgcggagccg
     2461 accaggtcat cccggtggat gtttacatcc ccggctgccc gccccggccc gaagctctcc
     2521 tttacggaat catgcagctc cagcgaaaga tccagcaggg aaacattgct gccggcagga
     2581 tcgaaccggt tgaccccgaa gaataccccg aacctccgaa agtttactga tcatccatta
     2641 aacttacctc aaatggataa tacaaatatc aaggaatttg tggccaggca cataccggaa
     2701 gccacattcg aagagaacaa gcaattcctc tcagcgacag tggaaccctc gaaacttcat
     2761 ggccttctga aagcgctgaa ggaaaatcct gaaaccctgt tcgattatct cttctgcatt
     2821 accggtgcgg atgtgcaagg caaactgatg gtgatctatc acctcgaatc gacaacattg
     2881 aagcaccagg tcgtcgtaaa aaccggcacg ccggatcgcg aaaatcctgt tttcgatagt
     2941 attactgatc ttttcagcgg agccgaattg cacgaaaatg agattttcga cctgttcggg
     3001 atcatcttta aagggcaccc gaaccttcgc cgcatgttca tgcccgatga ctggaagggc
     3061 tatccgctgc gcaaagatta caccgacgag gtcaatattg ttgaactttc ttaaccgaac
     3121 cgatatggaa gagaacacaa aaccaatgac ggcgccggaa acggaagagg agtatttcat
     3181 caatatgggt cctcagcacc cgtcgacgca cggtgtgctc cggttgcttg tctcccttca
     3241 gggtgagatc gtaaagcgtc tggatccgca cctgggctat atccaccgtg gtattgagaa
     3301 gatgtgtgaa aaagatacct atatccagat cattcacctg accgaccgga tggattacct
     3361 ctcggcgcac atgaacaacg aggctgtttg cctcctggtt gaagacgccc ttgagattga
     3421 agtcccggaa agggtcaaat acatccgcac catcatggcc gagctgcagc gtctgaattc
     3481 gcaccagcta tggtgggcca ccatggggat ggacctcggg gcgctgacca cttttttcta
     3541 tggtttacgc gaccgcgaaa agatcctgga cattttcgaa gagacctgcg gcgggcgttt
     3601 gctgctcagc tacaacactc cgggtggcgt gatgtacgat atccatccga atttccagaa
     3661 aagagtcaaa gagttcatca aatatttcag gaagaagatc agggattatg atgacatc
//
LOCUS       DAOPNH010000308         3707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29705_length_3707_cov_6.262322, whole genome shotgun sequence.
ACCESSION   DAOPNH010000308 DAOPNH010000000
VERSION     DAOPNH010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29705_length_3707_cov_6.262322"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKZ74_13105"
     CDS             complement(<1..1099)
                     /locus_tag="PKZ74_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM93739.1"
                     /translation="MIQRWPNLAVIIKTYLTVLAIFTGFRLFLFLTELRRIESFSGEL
                     GDIIRAFIMGVRFDVVISGYILALPALVLSVLFILNRHPQWIYKLFFWWVFILFTIAF
                     IVCAVDVPYFNQFFSRLTVAAFQWTDNMGMVMRMLLEEPRYYIILIPLILFIYFFIRI
                     LKRIYNKIPEQRSEVHVLLRIAVSLVLLGMVFIGIRGRIQAKAPIKVGTAYFSDNAFL
                     NQLGLNPVFTFMRSYLDVRDQRNRSVILMEPAKAVEKVREYLEISEPIGDYPVARQVL
                     ADSSEKKEYNVVLVLMESMSAANMARHGNKENLTPFLDSISHQGYYFENIYSAGIHTF
                     NGVFSTLFSFPAIYRQNPMRESTIFKYNGIGY"
     gene            complement(1096..2802)
                     /locus_tag="PKZ74_13110"
     CDS             complement(1096..2802)
                     /locus_tag="PKZ74_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPM93740.1"
                     /translation="MPENKSVRTIQSLTEKAWWKEAVVYQVYPRSFKDSDGDGVGDLK
                     GIIEKLDYIKSLGVDVVWLNPIFASPNDDNGYDISDYRAIMKEFGTMEDFDNLLQGMH
                     DRGIKLVMDLVANHCSSEHPWFVEARSSRENTFRDYFHWWPAEKGKPAKRYSFFDVNN
                     DAWKYDSLTDSYYLHYFSVKQPDLNWENPKVRTEIYDMMKFWFDKGIDGFRMDVIPFI
                     SKDTTFPELPAEYNGDMVRYYANGPHLHEYLQEMNREVLSKYDIMTVAEGAGITYREA
                     LDFVDPERKELNMAYHFDGMNIGYLPGRFKVMNPEGYDLLEFKRVYSLWDSVFAEKGW
                     GTIYLGNHDQPRMVTRWGNDSPEYRELSSKMLTTFLLTMRGTPYYYFGDELGMANIKF
                     ERIEDYRDIESINMYRQVKERGGDLQAFLEAQKISARDNGRTPFQWDDSPNAGFTAGT
                     PWLKVNPNYTGVNAAAQENDPNSVLNHFRKMVRLRKSEPALVYGHYTLLDPANPDVYA
                     YLREFEGTRILVVLNFKDKTAVTSLGIEMGQPEILAGNYPAPFSFRELRPYEAIVCKL
                     KD"
     gene            complement(3051..>3707)
                     /locus_tag="PKZ74_13115"
     CDS             complement(3051..>3707)
                     /locus_tag="PKZ74_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93741.1"
                     /translation="NSSANLRTAIEPCTLCDEGSVFCNTPSPYAGVGLSDDQRCFVCY
                     DNFYNVNSPIGKITFWGSDLTYTYNYVGCIPSEGKTFTFAFYENAEGAVGNLVTSFQA
                     IPAKAYCLPPFAYQDAPIFKYEVELPAPITMTDGWLSILGTGGTDGCWFGWCIASSGD
                     NFMYQACYSSFSYYELDLAFTLGPPPENPGVVIPISNWALMLALGLILTFAVFRFRKL
                     "
BASE COUNT          974 a          911 c          902 g          920 t
ORIGIN      
        1 tgtagcctat gccgttgtat ttgaagatgg tgctttcgcg catcgggttc tggcggtata
       61 tggccggaaa agagaacagg gtgctgaaaa ccccgttgaa ggtatggatc ccggcactat
      121 aaatattttc gaaataataa ccctggtgtg aaatgctgtc gagaaacgga gtaaggttct
      181 ctttgttccc gtgacgggcc atattggcag cgctcatgct ctccataagc accaggacga
      241 cattgtattc tttcttctcc gatgaatcgg ctaaaacttg ccgtgcaacg ggataatcgc
      301 cgatcggttc ggaaatttcc aggtactcac ggactttttc aaccgccttt gctggttcca
      361 taagaattac agaacggttt ctctgatccc tgacatccag gtagctgcgc ataaatgtaa
      421 agaccgggtt caacccgagc tggttcagga aagcgttgtc ggaaaaataa gcagtcccga
      481 ctttgatcgg cgcctttgcc tggatcctgc cccttatccc gatgaagacc attcccagca
      541 aaaccaggct caccgcaatc cttaacaaaa cgtggacctc ggatctctgt tcaggaatct
      601 tattataaat tctttttaat atcctgataa agaaataaat aaacaatatg agggggataa
      661 ggatgatgta gtaccggggt tcttcaagca acattctcat taccattccc atgttgtcgg
      721 tccactggaa agcggcaacg gtcaaccggg agaagaactg gttgaaatag ggcacgtcga
      781 ccgcgcaaac aataaaagca atggtaaaga gaataaatac ccaccagaaa aagagcttat
      841 aaatccattg aggatggcgg ttgaggatga aaaggaccga aagcaccagg gccggcaatg
      901 caagaatgta tccggaaatt acgacatcga acctgacacc catgatgaaa gcccggataa
      961 tatctcccag ttctccgctg aatgattcaa tccttcttaa ttccgtaagg aacaaaaaca
     1021 acctgaaccc ggtgaagatc gccaggacgg tcaggtaagt cttaataata acggccaggt
     1081 tgggccaccg ctgtatcaat cctttaattt gcatactatc gcttcatacg gacgtaattc
     1141 tctgaaagaa aagggggccg gatagtttcc ggccaggatt tcgggctgac ccatctctat
     1201 tcccaggctg gtcacagcgg ttttatcttt aaagttcagc acaaccaaaa tccgggtccc
     1261 ctcaaattcc cgaaggtagg catacacatc gggattggcg ggatcaagca aagtgtaatg
     1321 tccataaacc agggccggtt ccgatttgcg gagcctgacc atcttccgga aatgattcag
     1381 gacggagttc ggatcgtttt cctgtgcggc agcgttgacc ccggtataat tggggttgac
     1441 cttcagccag ggcgttccgg cggtaaaccc ggcattcggc gaatcgtccc actggaaagg
     1501 ggtacggccg ttgtcgcgtg ccgagatctt ctgcgcttcc aggaaggcct gcaggtcgcc
     1561 tcccctctcc ttcacctgcc ggtacatgtt gatcgactcg atatcgcggt aatcttcgat
     1621 acgctcgaat tttatgttag ccatgcccag ttcgtcgccg aagtaatagt aaggcgttcc
     1681 gcgcatcgtt agcaggaaag tggtcagcat tttggaagat aattcacggt actccgggct
     1741 gtcgttgccc cagcgggtca ccatccgggg ctggtcgtga ttgccaagat agatcgtgcc
     1801 ccagcctttt tcggcaaaga cactgtccca gagcgaataa acccgtttaa actccagcag
     1861 gtcgtagcct tcggggttca tcactttgaa ccggccgggc aggtagccga tattcatccc
     1921 gtcgaaatga taggccatgt tcagttcttt ccgctcagga tccacgaaat cgagggcttc
     1981 gcgataggtg atcccggcgc cttcggcaac ggtcatgata tcgtattttg aaagcacttc
     2041 cctgttcatc tcctgcaggt attcgtgcag gtgcggcccg ttggcatagt atctcaccat
     2101 gtcgccgttg tattcggccg gcagctccgg gaaagtcgta tccttgctga tgaaagggat
     2161 cacatccatc cggaagccgt cgatcccttt atcgaaccag aatttcatca tgtcgtagat
     2221 ctccgtcctg accttcgggt tttcccagtt caggtcgggt tgtttgaccg agaaatagtg
     2281 caggtagtac gaatcggtca ggctgtcgta tttccacgca tcgttgttaa cgtcgaagaa
     2341 gctgtaccgt tttgccggct tacccttctc ggccggccac cagtggaaat agtcgcggaa
     2401 agtgttttcg cggctgctgc gggcctccac aaaccagggg tgttcggagc tgcagtggtt
     2461 ggccaccagg tccatcacca gtttgatccc ccggtcatgc atcccttgca gaaggttatc
     2521 aaagtcctcc atcgtgccga actctttcat gatcgcccgg taatcactga tgtcgtaccc
     2581 gttgtcgtcg ttcggcgagg cgaagatggg attaagccag actacatcga caccgaggct
     2641 tttaatgtaa tcgagctttt cgatgatccc tttcaggtcg ccgacgccat cgccgtctga
     2701 atctttaaag ctgcgggggt agacctggta gacgaccgct tctttccacc aggctttttc
     2761 agtaagtgat tgaatggttc taactgattt attttcaggc atctgacagg aggagattaa
     2821 tatagatatc agaaagatga ttttacaaaa cgataatttg attttcatga taaattgata
     2881 aagccaatat tggaaccggc gcatcgggat gcaaagatat atccggaaat caaatgaaag
     2941 gaagaatcgt gcaaatccgt ttaatccgtc tcattcgtgt tccattaatt gaacacgaat
     3001 cacacggata aaacgaatta tcgcggatgt attatggaaa tgctgaccgt ttacagtttt
     3061 ctgaagcgga aaacggcaaa agtcaggatc aagccaagtg ccagcatcaa cgcccagttt
     3121 gagatgggga tgaccacgcc cgggttctcc ggtggcggac ccagcgtaaa tgccagatca
     3181 agctcataat aggagaaaga tgagtaacac gcctggtaca taaaattatc accggaagag
     3241 gcaatgcacc atccaaacca gcaaccatcc gtaccacccg ttcccagtat cgacagccat
     3301 ccatcggtca tggtgattgg agcaggcaat tctacttcat atttaaaaat tggagcatcc
     3361 tggtatgcaa aagggggcag gcaataagcc tttgcgggaa tagcctggaa agaggtaacc
     3421 aggtttccta ccgcgccttc cgcattctca taaaaagcaa aggtaaaagt tttgccttca
     3481 gaaggtatgc aacccacata attataagta taggtcagat cacttcccca gaatgtaatt
     3541 tttccaatag gtgaattcac attgtaaaaa ttatcgtagc acacaaaaca ccgctggtca
     3601 tcacttaatc caaccccagc gtaaggactt ggcgtattgc agaacacaga accttcgtca
     3661 cacaatgtgc atggttcgat agcggttctt aaatttgcac tgctgtt
//
LOCUS       DAOPNH010000309         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29744_length_3703_cov_6.073191, whole genome shotgun sequence.
ACCESSION   DAOPNH010000309 DAOPNH010000000
VERSION     DAOPNH010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29744_length_3703_cov_6.073191"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKZ74_13120"
     CDS             <1..492
                     /locus_tag="PKZ74_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPM93742.1"
                     /translation="FFYPDFSEEIHHEAELVLKINKVGKSIPEKYALDYIESIGLGFD
                     FTARDLQQKCKEKGLPWEISKAFDYSAPISPEFIPKDHFDDLRNIGFHLDVNGKTVQK
                     GNSADMIFSFEKIISYVSQFMTLRTGDLVFTGTPAGVGPVKIGDKMEGHLNDRLLLTC
                     LIK"
     gene            complement(485..775)
                     /locus_tag="PKZ74_13125"
     CDS             complement(485..775)
                     /locus_tag="PKZ74_13125"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPM93743.1"
                     /translation="MKSSNDRPLGDVIREIIETYRLEGKLNEIKILHSWENVVGNMIA
                     RHTKNLYIRNNKLIVKVDSPALKNELTYSSSLILEKLNAAAGCKVVDEIVFI"
     gene            complement(772..>1685)
                     /gene="recF"
                     /locus_tag="PKZ74_13130"
     CDS             complement(772..>1685)
                     /gene="recF"
                     /locus_tag="PKZ74_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPM93744.1"
                     /translation="DFFSLHGTYLKNGDSPDLVSCIQRSEQRKVFKINQKEYERLADH
                     IGLFPLVMVSPYDRDLINEGSEVRRRYLDGVISQFDKQYLDDLIRYNKALLSRNSLLK
                     QASEGKALNHSLIELLDMQLADYGQKIHEKRAGFLEAFSTVFQKYFAFISEGKESVTL
                     HYSSQLFSRSLGELLAVSLDKDRSARFTTQGIHKDDLEFRISGFPLKKFGSQGQQKSF
                     VIAIKLAQFDYTRDVKGFKPVLLFDDIFDKLDDKRVHQIIRLVSENSFGQVFLSDTQR
                     SRIEKLFEEVNIEHRIFFICEGKVEPL"
     assembly_gap    1686..1785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1786..2842)
                     /gene="porQ"
                     /locus_tag="PKZ74_13135"
     CDS             complement(<1786..2842)
                     /gene="porQ"
                     /locus_tag="PKZ74_13135"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPM93745.1"
                     /translation="MRTLIHYFILVLPLVFILEQAGGQVGGKGTYQFLNLPNSARIAA
                     LGGNFLSINDDDITLTLANPSLINEDMHNQLGFSFVDYYTDVNYGFVQYGRTFNKAGS
                     FVGTMQYIDYGKFQEADEAGNQYGTFHAGEYALNIGWGRKLTPRWAIGANFKMIYSGL
                     ASYHSFGLGVDVAGSYFSKDELFDASLLFRNIGYQVVAYQNGNHEPLPFEIQAGISKK
                     FEHIPFRIIALVTHLEKWDVRYESSVNPKGGLDPVTGEPNKISGVDEFADNLMRHVIF
                     GGEVTIAKTVSLQGSYNYQRRQEMKIPGKLSTVGFSWGIGIRVKQFQFNYARSAYHLS
                     GSPNFITVMIDMDGFPKK"
     gene            complement(2933..>3703)
                     /locus_tag="PKZ74_13140"
     CDS             complement(2933..>3703)
                     /locus_tag="PKZ74_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPM93746.1"
                     /translation="VSLDEPVGIDFPVNGFAVQDNGYQPPAEDGSAVNVIVDPESDRL
                     QLLAPFPAWNGEDLTGLHLLIKARGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAMN
                     DFNGKTNLVKNQVTGEYGEVPAVARFYKANGTGSIVVGDENYGEGSSREHAAMEPRHL
                     NVRAILVRSFARIHETNLKKQGMLALTFANPSDYDKIKEDDRMDILGLKEMAPGKPLS
                     IVLRHADGSVDQFPANHTYNKNQIEWFKAGAALNLFRE"
BASE COUNT          952 a          858 c          819 g          974 t
ORIGIN      
        1 ttcttttacc cggatttttc cgaggaaatt catcatgagg ccgagttggt actgaagatc
       61 aacaaagtag gcaaatccat cccggagaag tatgcgctgg attatataga gagcatcggg
      121 ctcggttttg attttacagc ccgtgacctg caacagaaat gcaaagaaaa aggcttaccg
      181 tgggaaatct caaaagcttt tgattattca gcgcctatca gtccggaatt tattccgaaa
      241 gatcatttcg acgatctgag gaacatcggt tttcatttag atgtaaacgg aaaaacagtt
      301 cagaagggaa attcagcgga tatgatcttc agctttgaaa agatcatttc gtatgtttca
      361 caattcatga ctctgcgtac cggagatctc gtatttacag gcacaccggc cggcgtcgga
      421 ccggtaaaga tcggggataa aatggaagga cacctgaacg accgcctgtt gcttacctgt
      481 ctgatcaaat aaaaacgatc tcatcgacca ctttgcaccc tgcagcggca tttaattttt
      541 ccaggatcag ggatgaagaa taggtcagtt catttttcaa ggccggcgaa tcgactttga
      601 cgataagttt attattccgg atgtacagat tcttagtatg gcgggcaatc atattaccga
      661 ccacattctc ccatgaatgc aggattttga tctcgttcag tttcccttca aggcgatacg
      721 tttcaatgat ctcccggatc acatctccca atggcctgtc gttactgctt ttcataacgg
      781 ttccacctta ccttcacaaa taaaaaaaat acggtgttca atattaacct cttcaaaaag
      841 tttttcgatc cttgacctct gggtatcgga taagaaaacc tgaccgaaac tattttcgct
      901 gaccagccgg ataatctggt gaacgcgttt atcgtcaagc ttgtcgaata tatcatcaaa
      961 caataaaaca ggtttaaatc ctttcacatc gcgtgtatag tcgaattggg ccagttttat
     1021 ggcaatgaca aatgatttct gctgtccctg tgaaccgaat ttcttcagcg gaaatccgga
     1081 aattctgaat tccaggtcgt ctttatgaat tccttgtgtg gtaaaccttg cagaacggtc
     1141 tttatcgagc gaaacggcaa ggagctcacc caggctccgg ctgaacagct gggaagaata
     1201 atgcaatgtc accgattctt taccttcgga aataaaggca aaatactttt gaaataccgt
     1261 ggagaatgct tccaggaatc cggctctctt ttcgtgtatt ttctggccat aatcggccag
     1321 ttgcatgtcc agtaattcga tgagggaatg attaagggcc ttcccttccg atgcttgttt
     1381 cagaaggcta ttgcggctca aaagagcttt attgtagcgg attagatcgt ccaggtactg
     1441 tttgtcgaac tgggagatca ctccgtcgag gtaccgcctt ctaacctcac tgccttcatt
     1501 gatcagatca cgatcataag gagaaaccat gaccagtggg aacaatccga tgtggtcggc
     1561 aagccgttca tactcctttt gattgatctt aaaaactttc cgttgctcgg acctttggat
     1621 acaggaaaca aggtcgggac tgtcgccatt cttcaggtaa gttccgtgca ggctgaaaaa
     1681 atcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcttt ttagggaagc
     1801 cgtccatatc aatcataacg gtaataaaat taggtgaccc ggagagatga taagccgaac
     1861 gggcataatt gaactggaat tgtttcaccc ggatcccgat gccccacgag aagcccacgg
     1921 tggaaagttt tccggggatc ttcatctcct gtcgtctctg gtagttataa gatccttgca
     1981 ggctgacggt tttcgctatg gtgacttccc ctccgaagat tacatgccgc atcaggttat
     2041 cggcaaattc atccacaccg ctgatcttat tcggttcgcc cgtgaccggg tcgagacctc
     2101 ctttgggatt gacactgctt tcgtagcgga catcccattt ttcaaggtgt gtgaccaggg
     2161 caatgatacg aaaagggatg tgttcgaatt tctttgaaat accggcctgg atctcgaaag
     2221 gcaaaggctc atggttaccg ttctggtaag ccacaacctg gtaaccgata tttctgaaca
     2281 gcaatgaagc atcaaagagt tcatctttgc tgaaataaga gcctgccacg tcaactccca
     2341 acccaaatga atgatacgaa gccagtccgg agtagatcat cttaaaatta gcgccgattg
     2401 cccacctcgg ggtcagtttc cttccccatc cgatattgag tgcgtattct ccagcatgaa
     2461 aggtgccgta ttgatttccg gcttcatcgg cttcctggaa cttgccgtaa tcgatatact
     2521 gcatggtgcc gacaaaactc ccagctttat taaatgtcct cccgtattgc acaaaaccgt
     2581 aattcacatc tgtataataa tcgacaaaac tgaaccctag ctggttatgc atatcttcat
     2641 tgatcaatga agggtttgcc agggtcaagg taatatcatc atcgttaatg gataggaagt
     2701 tgcctccaag agcggcaatt ctggctgagt tcggcaggtt caggaactgg taggtgcctt
     2761 tgcctcccac ctggccgccg gcttgttcca gaataaaaac cagcggaagg accaggatga
     2821 aataatggat taaagttctc aatgagatca gtttttggtt ccaatccata aacggtaatt
     2881 acttaaaaat attatatcct cgcaaaacca attatcccca actcactatt cactattccc
     2941 tgaaaaggtt aagcgcagca ccagccttga accattcaat ctgattttta ttatacgtgt
     3001 ggttcgccgg gaattgatcg accgaaccat cggcgtgccg gagaacaatc gaaagaggtt
     3061 tgcccggagc catctctttc agcccaagga tatccatccg gtcatcttct ttaatcttat
     3121 cataatcaga aggattagca aatgtcaaag caagcatccc ctgctttttg agattagtct
     3181 cgtggatacg agcaaacgaa cggaccagga ttgccctcac attcagatgg cggggctcca
     3241 tagcggcatg ttcacgcgat gagccttcac catagttttc atcgcccaca acgattgagc
     3301 ccgttccgtt tgctttgtag aaccgggcaa cggcaggcac ttcaccgtat tcaccggtta
     3361 cctggttttt caccaggttg gtcttgccgt taaaatcatt catagcgccg atcagcaggt
     3421 tgttggagat attgtcgaga tgaccgcggt atttgagcca gggaccggcc atactgatgt
     3481 ggtcggtcgt gcatttgccc cgtgctttaa taagcaggtg cagccctgtc aggtcctcgc
     3541 cattccaggc agggaacgga gcgagcagct gtagccggtc ggattcaggg tcgacgatca
     3601 cattcacggc gcttccgtct tctgccggag gctgatagcc gttatcttga accgcaaaac
     3661 cgttcaccgg aaagtcgata ccgacgggct catccaggct gac
//
LOCUS       DAOPNH010000310         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29925_length_3685_cov_7.592011, whole genome shotgun sequence.
ACCESSION   DAOPNH010000310 DAOPNH010000000
VERSION     DAOPNH010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29925_length_3685_cov_7.592011"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PKZ74_13145"
     CDS             <1..1959
                     /locus_tag="PKZ74_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM93747.1"
                     /translation="AYSSALNLDPLTPLYETNPDTLSLPPFSEQPVVKTPDGKVYGIS
                     DYLDAEVVNPIALLEIQTGETRVDKFVGNIFGEITFMEGLKFRSTLGTDLAYVHWDTY
                     GPLYFLNGSQSNTDKTNVSKSMDRYFNWQWENTLTYTKKIKDHSFSLLAGTSAKEDNY
                     ENLSGFNADVPITDPDNVYLNMATDTSWRANGGARDYALFSVIGRVTYDFKSRYAFTG
                     IIRRDGSSNFGPNNRYGWFPSVGVSWLLTEEKFIPELGPVDFLKLRASWGINGNDRIG
                     YYQYVSTIDKSRGYIFGGATNTGASPGYIENADIKWEESEQIDIALDAGLFNNRLTAT
                     FDYYIKNTIDLLETIEIPGYVGNNPPVANVGSVRNEGVEMSINWRHYKEKIRYYAGVN
                     AAFNKNKMTEIANEQGFLTGATWAVAGFVTRCEEELPIAYFWGYKTDGIFQNENEVFQ
                     HYGTDGVPLQPNAKPGDVRFIDVNGDNKINEEDRTMIGNPTPDWTLGINGGIEYMNFD
                     LSFLFTGAFGHQIFNGAQRQDLFYTNRTTAILDRWTGEGTSDDIPRMAWVDVNNNYRV
                     SDLYIEDGDFLRLKNLQIGYTLPKKILDRIKSGAWRFYISAENLVTFTKYTGADPEIG
                     AISSLDIGIDRGMYPSAVTFRFGTSISF"
     gene            1978..3534
                     /locus_tag="PKZ74_13150"
     CDS             1978..3534
                     /locus_tag="PKZ74_13150"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM93748.1"
                     /translation="MKKSIIYPISIIVLVMLTIGCSKDFLDRQPLDREVSTNFYRTEA
                     DAMMALAAAYDALGWDQMPGVSWAPFCTVSDILSDDSYAGGADANDGMDENELNNFTI
                     PTTNLLVKATWMKNYVGIYRANLLMEKMDQITMADEKKARMIAECKFLRAYYHFDLVR
                     LFENIPLLTATIKGPSEYNQDQETPKEVYDQIALDLVEAAAVLPATIPADESGRVSKW
                     AAQSLLGRVYLFYNGVYSADLVAGDMTIDRAKALEYLEDVITNSGHDLFPEYSTNFRL
                     AGEYGIESVFEISHGDTPAWWDWNYVVGGEGNLAAQMQGPRVTNSSNWDRGWSFGVVS
                     QKLVDDFGDDPRLAFTIVKEEELDGTLTKGFQHTGYYSRKYSSDAEHWGSDGQFELNR
                     TCNFRVIRFSDVLLMAAELGSPNAQNYLDRVRSRVNLGSIPATPENIYRERRLELSLE
                     GIRYFDVLRKGIAFASQEFTVVDVRGPKYTDEQVVYDHTFNSATKGFLPIPQTEIDMS
                     AGVLKQNEGY"
BASE COUNT          953 a          984 c          910 g          838 t
ORIGIN      
        1 gcctacagca gcgcccttaa cctcgatcct ttaacaccgc tctatgaaac caatcccgat
       61 acgctcagcc tgcccccgtt ttcagaacag ccggtggtga aaaccccgga tgggaaagtt
      121 tatggcatat ctgattacct tgatgccgag gtagttaatc ctattgcttt actcgaaatc
      181 cagaccggag aaacccgggt tgataaattc gtcggaaaca ttttcggtga aatcaccttt
      241 atggaagggc tgaagttccg gtcaaccctc ggaaccgacc tggcctatgt ccattgggat
      301 acctatggcc cgctctattt tctgaacggc tcccagtcaa ataccgataa gaccaacgtt
      361 tccaaatcga tggaccgcta tttcaactgg cagtgggaaa ataccctgac ctacaccaaa
      421 aagattaaag atcattcttt ctcgctattg gccggtactt ctgctaaaga agacaattat
      481 gagaacctga gcggttttaa tgctgacgtc cccattaccg atcccgataa tgtttacctc
      541 aacatggcca ccgacacctc ctggagagcc aatggcggcg ccagggatta tgcgttgttt
      601 tccgtgatcg gcagggtcac ctacgacttt aaaagcagat atgccttcac cgggatcatc
      661 cgccgtgacg gatcctccaa cttcggcccg aacaaccgtt acggttggtt cccgtcggta
      721 ggcgtatcct ggctgctgac ggaggagaaa ttcatccccg aactgggtcc cgttgatttc
      781 ctcaaactca gggcttcatg gggtattaac ggaaatgacc gtatcggtta ttatcagtat
      841 gtttcaacca tcgataagag ccgcgggtat attttcggag gcgcaacaaa taccggtgct
      901 tcgcccggat atatcgaaaa tgccgatatc aagtgggaag aatcggaaca gatcgatatc
      961 gcactcgacg ccggattgtt caacaacagg ctgaccgcaa cgttcgatta ctacattaaa
     1021 aataccatag acctgctgga aaccattgag attcccggat atgtgggtaa caacccgccg
     1081 gttgccaacg tgggcagcgt caggaacgaa ggggtcgaaa tgtcaatcaa ctggaggcat
     1141 tacaaagaaa agatccgcta ctatgccggg gtcaatgcag cctttaacaa gaataaaatg
     1201 acggaaatcg ccaatgagca gggattcctt actggagcta cctgggcggt agccggattt
     1261 gtcacccgct gcgaagaaga gctgccgatt gcctatttct ggggctataa aaccgacggc
     1321 attttccaga atgaaaatga agtcttccag cattacggta ccgacggcgt tccgctgcaa
     1381 cccaatgcaa agccgggcga cgtcaggttc attgatgtca atggcgataa taaaatcaac
     1441 gaggaagacc gtacgatgat cggtaatccc acccctgact ggacgctggg aatcaatggt
     1501 ggcatcgagt atatgaactt cgacctctcc ttcctgttta ccggcgcttt cggccaccag
     1561 atctttaacg gtgcccagcg ccaggattta ttttatacca atcgtacaac ggctatcctc
     1621 gacaggtgga ccggtgaagg gacatccgat gatattccac gcatggcctg ggttgacgtc
     1681 aataataatt acagggtctc cgacctctat attgaggatg gagatttcct gcgcctgaag
     1741 aacctccaga tcgggtatac cctccccaaa aagatcctgg accgtataaa aagcggcgcc
     1801 tggagatttt atatctctgc tgaaaacctt gtcactttta ccaaatacac cggtgcagat
     1861 cccgaaattg gcgcgatcag ctccctggat atcggtatcg accggggcat gtacccatcg
     1921 gccgtgactt tcagattcgg aaccagtatt tctttctaat cttaaaaaca aggaaaaatg
     1981 aaaaaatcaa taatataccc gatttcaatc atagtactgg tcatgctgac aattggatgc
     2041 agcaaggatt ttcttgaccg tcagccgctc gaccgggaag tcagcacgaa cttttaccgg
     2101 accgaagccg atgccatgat ggcactggcc gcagcatacg acgcacttgg atgggaccag
     2161 atgcccggcg tttcatgggc ccccttctgc accgtttccg acatcctttc cgatgattct
     2221 tacgccggtg gcgcagacgc taacgacggc atggatgaaa acgagctgaa taactttacc
     2281 attccaacga ccaacctgct ggtcaaggca acgtggatga agaactacgt gggaatttac
     2341 agggctaacc tgctgatgga aaaaatggac cagatcacga tggccgacga aaagaaagcc
     2401 cggatgatcg ccgaatgcaa gttcctgcgg gcttactacc atttcgacct ggtcaggctc
     2461 tttgagaata tcccgctgct gaccgctacg atcaaaggcc cgtcggaata caaccaggac
     2521 caggaaacac cgaaagaagt ctatgaccag atcgccctcg acctcgtcga agcggcggca
     2581 gtccttcccg caaccatccc ggccgatgag tccggcaggg tgagcaaatg ggcagcccag
     2641 tcgcttctcg gcagggttta cctgttctat aacggtgttt acagtgccga cctggtggcc
     2701 ggcgatatga caatcgaccg ggccaaggcg ctcgaatatc tcgaagatgt cattaccaac
     2761 agcggccacg acctcttccc ggaatattct accaatttca gactggccgg cgaatatggc
     2821 atcgaatcgg tgtttgagat ctcccacggc gataccccgg catggtggga ctggaattat
     2881 gttgtcggag gtgaaggcaa cctggctgcc cagatgcagg gccctcgcgt taccaattct
     2941 tccaactggg accgcggctg gagcttcggt gtcgtcagcc agaaactggt tgatgatttc
     3001 ggcgacgatc cccgcctggc cttcacgatt gtgaaagaag aagaactgga cggcacgctc
     3061 accaaaggat tccagcacac aggctattac tcgcggaaat acagctccga tgccgaacat
     3121 tggggttccg acgggcagtt tgaactgaac cgtacctgca atttcagggt gatccgcttc
     3181 tccgatgtgc tgctgatggc cgccgaattg ggcagcccga acgcgcagaa ctatctcgac
     3241 agggtcagat caagagttaa cctgggaagc atcccggcca cgcccgaaaa tatctaccgt
     3301 gaacgccgcc tcgaactttc cctcgaaggt atccgctatt tcgacgtgct tcgcaaaggg
     3361 attgcttttg cttcccagga attcaccgta gtggatgtgc gcggcccgaa atataccgac
     3421 gaacaagtcg tatacgacca cacctttaac tcagccacga aaggcttcct gcccatcccc
     3481 cagaccgaga tcgatatgtc ggctggagtg ctgaagcaga atgaaggata ttgatcaaga
     3541 caggctgtct taaaagtcca taaattcact tttttaggta gtccgaccac taaagccgtc
     3601 ggttcctgag ggattttcct tccgacgact aaagccgtcg gttccatctt tgtaatattc
     3661 ttggaaccaa cggctttagt cgttg
//
LOCUS       DAOPNH010000311         3678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_29991_length_3678_cov_7.541540, whole genome shotgun sequence.
ACCESSION   DAOPNH010000311 DAOPNH010000000
VERSION     DAOPNH010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29991_length_3678_cov_7.541540"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKZ74_13155"
     CDS             <1..1009
                     /locus_tag="PKZ74_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HPM93749.1"
                     /translation="FIDPRNKQLSSLLIGSGLPGGMMGSLMADLKGAQRSINTSLKGR
                     GIPELSEDELMVRMFNEVEYIWPKLGYPPLVTPFSQYVKNMAIMNILQMAGGKERYTM
                     IDNNTWDMILGKAGKLPGPLAPEIVTLAGEQGKEFYQGVPQDAFPDELEKYRKEMTAN
                     NWDTGPDEEELFEFAMHDKQYRDFKSGIAKTRFEAELAKAKELAEGPKNMPAAPEPAP
                     APAPVKEKPVEEKPKEVDPDIREITAPAKGQIIYNLFGEDTEFSTIGEHISPGDRLCY
                     IQTSAYIGEITCPYNGIISEIVVQQGVQVKKGEVIFRIKEVVGEIKPKTPKKKSQTAG
                     K"
     gene            complement(1038..1922)
                     /gene="ygiD"
                     /locus_tag="PKZ74_13160"
     CDS             complement(1038..1922)
                     /gene="ygiD"
                     /locus_tag="PKZ74_13160"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HPM93750.1"
                     /translation="MDRRSFLQSVSVLGLTGITMKLNALNYASGTFGNTSKMPALFLG
                     HGSPMNAIEENEFVRGFREMGKALPRPNAILCVSAHWETRGTWVTAMDKPRTIHDYGG
                     FPRELYQVRYPAPGNPDLAFESKRLITSADVGLDHQWGLDHGCWSVVKHLYPDADIPV
                     IQLSLDQTKDAGYHYNLAKELSSLRNKGVLIVGSGNMVHNLRMVAWDRLNDVEFGYDW
                     AIEAGTRMKEYILSGDHQRLIDYKSQGKEFEMAIPTPEHYLPLLYVLALKEGNEEVGL
                     FNDKAIAGSLTMTSAIVR"
     gene            complement(1935..2471)
                     /locus_tag="PKZ74_13165"
     CDS             complement(1935..2471)
                     /locus_tag="PKZ74_13165"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM93751.1"
                     /translation="MSQQTNWISDPKHSEILFKVKHMMITNVKGEFRKFHIEADSDGE
                     QFIASSIRVVVDTFSVFTNVDDRDNHLRSADFFDVENYKEMVFNGSSLKKTGDGNFEL
                     TGILNIRGVSKEVKLDVEFGGINKDPWGNEKAGFSISGKINRKEWGLNWNAALEAGGV
                     LVSDEVRISAEVQLVKTS"
     gene            complement(2689..3567)
                     /locus_tag="PKZ74_13170"
     CDS             complement(2689..3567)
                     /locus_tag="PKZ74_13170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM93752.1"
                     /translation="MRKFSIIDEHDPALIHYLDELPLWSAPFALQILDKVIPAKNLRV
                     LDIGFGTGVPLIELASRLGNSCKIYGIDPWKEACDRAGEKIRLFGINNITLFNAPAET
                     LPLGDNSVDLIISNNGLNNVDDMEQVLKESFRVLVPSGKLIFTMNTEETMTEFYSIMQ
                     EVLSEYNLAASVTAMKNHIRQKRKPLPEVAGLLEKSGFIITSVDHDWFHYTFTDGTAL
                     FSHFFIRLAFLGPWLKTVPKNYQQTIFSDIENRINTISSEKGHFRLTIPFVTMTSEKS
                     DNSIISHSCQTFGFPD"
BASE COUNT          981 a          832 c          888 g          977 t
ORIGIN      
        1 gtttatcgat ccacggaata agcagctctc ctcactgctc atcggcagcg gtttgccggg
       61 cgggatgatg ggaagcctga tggccgacct gaaaggagcc caaaggagca tcaatacctc
      121 gttgaagggg agaggaatac cggagctgag tgaagatgaa ctgatggtca ggatgttcaa
      181 cgaggtggaa tacatctggc cgaagctggg ttatccgcca ttggttaccc ctttcagcca
      241 gtatgtcaag aacatggcta tcatgaatat tttgcagatg gcaggtggaa aagagaggta
      301 cacgatgatc gacaacaata cctgggatat gatcctgggt aaggccggaa aactgcccgg
      361 accgctggcg ccggaaatcg ttacgctggc cggagaacaa ggaaaggagt tctaccaggg
      421 cgtaccccag gacgcatttc cggatgaact ggaaaaatac aggaaagaga tgacggctaa
      481 taactgggat acgggtccgg atgaagaaga gttgttcgag tttgccatgc atgacaaaca
      541 gtacagggat tttaagtcag gcatagccaa aacccggttt gaggctgaac tggcaaaagc
      601 gaaggagctg gcggagggcc cgaagaacat gccggctgct ccggaacctg cacctgctcc
      661 tgctcctgtg aaggaaaagc cggtggaaga aaaaccaaaa gaggtcgatc ccgatatccg
      721 cgagattact gctcctgcca aaggccagat catttacaat cttttcgggg aggataccga
      781 gttttctacc ataggcgaac atatatcacc gggcgaccga ctgtgctata tccagacctc
      841 ggcctatatt ggcgagatca cctgccctta caacggcatt atttcggaaa tagtggtcca
      901 gcagggagtt caggttaaaa agggagaggt tatattcagg ataaaagagg ttgttggcga
      961 aataaaaccc aaaacgccga agaaaaagtc tcaaacggca ggcaaataaa agactttaga
     1021 catcttgttc tggatgatta tcttacaata gccgaagtca ttgtcagtga gccggcaatg
     1081 gctttatcgt tgaacaaacc cacctcttca tttccttctt ttaaagccag gacatacaac
     1141 aacggaaggt aatgctcagg tgtcgggatg gccatttcaa attctttacc ctgggattta
     1201 taatcaatta atctctgatg atctccggac agaatatatt ctttcatccg ggtaccggct
     1261 tcaatcgccc agtcgtagcc gaattccaca tcattcagcc tgtcccatgc caccatccga
     1321 aggttatgta ccatattgcc gctgccgacg atcaggactc ctttgtttct gagcgatgat
     1381 agttcttttg ccagattgta atgatatcct gcatctttag tttgatcaag gcttaactgg
     1441 ataaccggga tatccgcatc cgggtaaagg tgttttacaa cactccagca accgtggtca
     1501 aggccccatt gatgatccag gcctacatcg gcagaggtga tcaatctttt agattcaaac
     1561 gccagatcag gatttcccgg tgcggggtac cgcacctggt aaagctcacg ggggaatccc
     1621 ccgtaatcat ggatggtcct cggcttgtcc atggccgtaa cccatgtgcc gcgggtttcc
     1681 cagtgggcgg aaacgcagag tatggcattc ggcctgggca gggctttccc catttccctg
     1741 aaacctctca caaattcatt ctcttcaatg gcattcatag ggcttccgtg acccaggaag
     1801 agcgccggca tctttgatgt gttgccaaaa gtgccgctgg catagttcaa tgcattaagc
     1861 ttcatagtga tccccgtcag ccctaacact gaaacagact gaagaaatga tctgcggtcc
     1921 atatcctgaa tttttcacga agttttaacc agctggacct cggcgctgat cctcacctca
     1981 tcgctgacca gcactcctcc ggcttcgagg gctgcattcc agttgaggcc ccactctttg
     2041 cggtttatct tgccgctgat agaaaaacct gctttttcat taccccaggg gtctttgttg
     2101 ataccaccga attcaacatc aagtttcacc tctttgctta ctcccctgat attcaggatt
     2161 ccggtcagct caaaattgcc atccccggtt ttcttcaaag aagaaccatt gaaaaccatt
     2221 tccttatagt tttcgacgtc gaaaaagtcg gcgcttctca gatggttgtc acgatcgtcg
     2281 acgttcgtga agactgagaa ggtatccacc actacacgaa ttgaagaagc gatgaactgt
     2341 tctccgtcgg aatcggcttc gatatgaaat ttcctgaatt cacccttaac gttggtgatc
     2401 atcatatgtt tgactttgaa caaaatttcg ctgtgcttgg gatcagagat ccagtttgtt
     2461 tgttgtgaca tcagatataa tttttaattc ttaattttta attcttaatt tttaattctt
     2521 aatttttaat tcttaatgta aatttctttt aagggctttc agagccacaa aatcaggaaa
     2581 cacgaaaatt catgaaaaac cattataaag atgaccggtt tttataagat tttgtgcttt
     2641 aaactataat gaaaatcttg acggattgtt cacacggaaa atgatttttt aatcgggaaa
     2701 accaaaagtt tggcacgaat gtgaaataat ggagttatct gatttttccg aagtcatggt
     2761 tacgaaaggt atagtcagac gaaaatgtcc tttttcggac gagattgtgt ttatcctgtt
     2821 ttctatgtcg ctgaatatgg tctgctgata attttttgga acggttttga gccagggtcc
     2881 gagaaaagcg agtcggatga agaaatggct aaaaagggca gtgccgtcgg tgaaggtata
     2941 atggaaccag tcgtggtcaa ctgatgttat tatgaatccc gatttctcaa gcaggccggc
     3001 aacttcgggc aaaggttttc ttttttggcg gatatgattt ttcatggctg tgaccgaagc
     3061 tgccagatta tattccgata agacttcctg catgattgag taaaactccg tcatagtttc
     3121 ttcggtattc atggtaaaga tgagtttacc gctcggtaca aggacacgga aagactcttt
     3181 cagcacctgt tccatatcat cgacattatt cagcccgtta ttgctgatga taagatcgac
     3241 agagttatcg cccaggggaa gggtttctgc cggcgcattg aacagggtga tgttattgat
     3301 cccgaacagt ctgattttct cgccggctct gtcgcacgcc tctttccacg gatcgatgcc
     3361 gtagatttta catgaattgc ccaggcggga agccagttcg atcagcggta cccctgtgcc
     3421 gaacccgata tcgagaacgc ggaggttttt agccgggatc actttatcga ggatttgcag
     3481 ggcaaacggt gcagaccaaa gcggcagttc atccaggtaa tggatcaggg cagggtcatg
     3541 ttcgtcgatg atagaaaatt ttctcatgag actccatctc atttatttcg agactccatc
     3601 tcacccatcg ggatggagtc ttatatgtgc gcccggcatg ggcgataact atagggtgaa
     3661 agacccgaac acacttgg
//
LOCUS       DAOPNH010000312         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_30022_length_3675_cov_6.851105, whole genome shotgun sequence.
ACCESSION   DAOPNH010000312 DAOPNH010000000
VERSION     DAOPNH010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30022_length_3675_cov_6.851105"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PKZ74_13175"
     CDS             <1..1534
                     /locus_tag="PKZ74_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPM93753.1"
                     /translation="MTSFNEVDGIPASGNRWLMTEVLRDQWGFDGFVVTDYTAINEME
                     AHGMGDLQDVSALALKAGVDFDMVGEGFLTTLEKSLEEGKVTKAEIDLACRRMLEAKY
                     KLGLFEDPYRYCDFERAKNEISTPEHKRIAREAATQTFVLLKNDGQALPLEKKGKIAL
                     IGPLADNRFNMAGMWAVACDHEQSMTVLEGFRNVLGDKAEVLYAKGSNIVDDPEYDKR
                     FNWGQSGVDPRTPEQMRQEAVALAARCDVVVAVMGEGAEMSGESSCRTEIGLPGSQQE
                     LLKALLKTGKPVVMVLFTGRPLGLNWEQENIPAILNVWFGGTESGNAIADVVFGDVNP
                     SGKLPVTFPQNVGQVPLYYNHKNTGRPLGESVWYQKYRSNYMDVTNDPLYPFGYGLSY
                     TTFTYGDLKIDRTELSAKKTVTASIDVTNTGNIDGAEVVQLYIRDKVGSITRPVKELK
                     GFQKIFLKAGETKTVTFTITPEDLKFYNSDLVYDWEPGEFEIMAGGNSREVASQLVSW
                     MK"
     gene            1643..1867
                     /locus_tag="PKZ74_13180"
     CDS             1643..1867
                     /locus_tag="PKZ74_13180"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HPM93754.1"
                     /translation="MPTAVRISDDLINSARKISKVENRSLTGQIEYWAKIGRIAEANP
                     DLSYKLIKDILTGMNEVESGEFSDYQMNEP"
     gene            1867..2142
                     /locus_tag="PKZ74_13185"
     CDS             1867..2142
                     /locus_tag="PKZ74_13185"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPM93755.1"
                     /translation="MKIIQSGAFRRKVKRFNTRQKLQLDEAIRNIIKNPSLGTRKKGD
                     LGSVYIHKFYIDKTLYLLAYSFSADQLELIMLGPHENYYSYLKTYLK"
     gene            complement(2156..3061)
                     /locus_tag="PKZ74_13190"
     CDS             complement(2156..3061)
                     /locus_tag="PKZ74_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM93756.1"
                     /translation="MDNRILLADNFSLSRIIHGQMRMNDWKMTKIELLDLFHELLDLG
                     ITTFDHADIYGNYTCEAKMGEVLAMEKGLREKIEIVTKCGIKLVSDKFPERKVKHYDY
                     SYEHIVTSVSNSLKNFGTDRIDLLLLHRPAPFFDPDPVAKAFSDLKKQGKVLHFGVSN
                     FLPGQFELLNSFTDENLVTNQVEISPYCLEHFENGNIDFFLKEKIKPMAWSPVAGGRL
                     LNPHDGKGPRIMRVIREIADELNTSSVDKVIYAWLLKHPVQMMPVVGTGRIGRIREAV
                     AALRLDMTLEQWYRIYIAAMGKELA"
     gene            complement(3071..>3675)
                     /locus_tag="PKZ74_13195"
     CDS             complement(3071..>3675)
                     /locus_tag="PKZ74_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM93757.1"
                     /translation="GNNDEVTCEVTSNDICASPATVNSDMIVITVFPAFEITCSAEDS
                     ICSGSPVILTPGSGFAAYLWNDGSSTESVTVEEPGVYWVMVTDGNGCTATDSVLLWPC
                     EVMPEIYAPTAFTPNGDGRNDRFLLFSTSPDAVFDFEISIYSRWGQLIFRSKDISEGW
                     DGTLNGVPSPSEVFTYFARFRTGSANGNTQTMAGSVTLIR"
BASE COUNT          939 a          897 c          996 g          843 t
ORIGIN      
        1 gatgacatct ttcaacgaag ttgacggcat cccggcatcg ggcaaccgct ggctcatgac
       61 tgaagtattg cgcgaccaat ggggcttcga cggatttgtt gtgaccgact acacagcaat
      121 taatgagatg gaagcacacg gaatgggaga tttgcaggat gtttcggcgc ttgcactgaa
      181 agccggtgtc gatttcgaca tggtcggcga agggttcctg acgacattgg agaaatcgct
      241 ggaggaaggg aaagtgacaa aggcggagat cgacctggcg tgccgacgca tgctggaagc
      301 caaatacaag ctgggattat tcgaagatcc ctaccggtac tgcgatttcg aacgggctaa
      361 gaatgagatc tccaccccgg aacacaaacg gattgcccgg gaagccgcaa cccaaacgtt
      421 cgttctgctt aaaaatgacg gccaggccct gcccctggag aaaaagggaa agatcgcact
      481 gatcggtccg ctggccgaca accgcttcaa tatggcgggg atgtgggccg tagcctgcga
      541 ccatgagcaa tcgatgacgg tgctggaggg tttccggaat gtgctgggcg acaaggccga
      601 ggtcctttat gccaaaggga gcaatatcgt cgacgatccg gagtatgaca agcggttcaa
      661 ctggggccag tcgggcgttg atccccgcac cccggaacag atgcggcagg aggccgttgc
      721 cctggctgcc cgctgcgatg tggtggtggc cgttatgggc gagggtgcgg aaatgagcgg
      781 agagagctcg tgccggacag agatcggcct gccgggaagc cagcaggaac tgctgaaagc
      841 attgctgaaa acgggaaagc cggtcgtgat ggtcctgttc accggccgtc cgctggggct
      901 caactgggaa caggagaaca tcccggccat cctgaatgtc tggtttggag gtacggaaag
      961 tggcaacgcc attgccgacg tggttttcgg cgatgtgaac ccttcgggga agctgccggt
     1021 gacctttccg cagaatgtcg gccaggtgcc gctttattac aatcacaaga ataccggccg
     1081 cccgctgggc gagagcgtgt ggtaccagaa ataccgctcg aattacatgg acgtgaccaa
     1141 cgatccgctg tatccgttcg gctacgggct cagctacacc acatttacct acggtgatct
     1201 gaagattgac cggacagagc tttctgccaa gaagacggta acggccagca tcgacgtgac
     1261 caataccggc aacatagacg gcgccgaagt agtacagctt tatatccgcg ataaggtcgg
     1321 cagcatcacc cggccggtga aggagctgaa aggatttcag aagatattcc tgaaagcggg
     1381 tgaaacaaag accgtgacct ttacaattac gccggaagac ctgaagttct ataattcgga
     1441 cctggtctac gactgggagc cgggcgagtt cgagatcatg gccggcggga actcaaggga
     1501 ggtggcttcg caattggtga gctggatgaa gtagaggatt taacgattgg gcgatttgac
     1561 gatttgggga tttggggatt ttatggcata aaaatagcaa acagttgcat tttgtagtat
     1621 atttgtacca aattgtttca ttatgccaac agcagtcaga atatcggatg atttgataaa
     1681 ctctgccaga aaaatcagta aggttgagaa ccgttcactg acagggcaaa tcgaatactg
     1741 ggcaaaaatc gggcgtattg cggaagccaa tcccgatttg agttataagt tgatcaaaga
     1801 tattctcacc ggaatgaacg aagtagaatc gggagaattt tctgattatc agatgaatga
     1861 accctgatga aaattatcca gtcaggcgca ttcaggcgta aagttaaacg attcaatact
     1921 cgtcagaaac tacaacttga tgaggcaatc aggaatatta ttaagaatcc atcattgggc
     1981 acaaggaaaa agggagatct gggatcagta tatatccata agttctacat cgacaaaacc
     2041 ctttatctgc ttgcctattc cttttcggcg gaccaacttg agttgatcat gctcggacct
     2101 catgagaatt attattcgta cctgaaaact tatttgaaat aggtaattat ccaaatcacg
     2161 ccagttcttt ccccatcgcg gcgatataga tccggtacca ctgttccagg gtcatgtcga
     2221 gccggagtgc ggcaacggct tccctgatcc gcccgatacg tccggttccg acaacgggca
     2281 tcatctgcac cgggtgtttc agcagccagg catagatcac cttatcgacc gatgatgtat
     2341 tcagctcatc cgctatctcc ctgatcaccc gcatgatccg cgggcctttc ccgtcgtgag
     2401 ggttcaggag acgaccgccg gccacgggcg accacgccat cggcttaatc ttttctttca
     2461 aaaagaaatc gatgttacca ttctcaaaat gctcgaggca atagggcgag atctccacct
     2521 ggttggtgac gagattttca tccgtaaaag aattcaaaag ttcaaactga cccggtaaga
     2581 aattggaaac gccgaaatgc agcaccttcc cctgcttttt caggtcggag aaggcttttg
     2641 ctaccggatc aggatcgaag aagggcgccg gacggtgcag caaaagcaga tcgatccggt
     2701 cggtgccgaa gtttttcagc gaattgctga ccgatgtgac gatatgctca tagctgtaat
     2761 cgtagtgttt gactttccgt tccgggaatt tatccgaaac cagcttgatc ccgcatttgg
     2821 tgacaatttc gattttttcg cggagcccct tttccattgc cagcacctcc cccatcttcg
     2881 cttcacaggt ataattcccg taaatgtcgg catggtcgaa cgtggtgata ccaaggtcaa
     2941 ggagttcgtg aaacaggtcc agtaattcaa ttttggtcat tttccagtcg ttcatccgca
     3001 tctggccgtg gatgatgcgc gacaggctga aattgtctgc cagtaaaatt ctgttatcca
     3061 taatgaaggt ttatctgatc aatgtcaccg aaccggccat ggtttgtgtg ttgccattgg
     3121 ctgacccggt cctgaacctg gcaaaatatg taaagacttc cgaaggagag ggaacgccat
     3181 tcagggtgcc atcccaccct tccgagatgt ctttcgaacg gaagatcaat tgaccccatc
     3241 ggctgtatat gctgatctcg aagtcgaaaa cggcatccgg agaggtactg aacagcaaga
     3301 accggtcgtt gcggccgtcg ccgttcgggg tgaatgctgt cggggcatag atctccggca
     3361 tcacttcaca gggccatagc aaaacggaat cggttgcggt acagccgttt ccatcggtca
     3421 ccatcaccca gtaaacaccc ggttcttcca ccgtgaccga ttctgtactg ctgccatcat
     3481 tccataaata agccgcaaat ccgcttccgg gcgttaagat tacaggtgat ccgctgcaga
     3541 tggagtcttc ggccgaacag gtgatctcga atgccgggaa tacggtaatc acaatcatat
     3601 cgctgttcac cgtagccgga gacgcacaaa tgtcatttga agtcacttca caggtcactt
     3661 cgtcattgtt tccgg
//
LOCUS       DAOPNH010000313         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_30043_length_3673_cov_5.644555, whole genome shotgun sequence.
ACCESSION   DAOPNH010000313 DAOPNH010000000
VERSION     DAOPNH010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30043_length_3673_cov_5.644555"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PKZ74_13200"
     CDS             <1..848
                     /locus_tag="PKZ74_13200"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPM93758.1"
                     /translation="INGHKVKMLSANNPEDIDYEAEGIHNALLIDSTGVWTKREDLAR
                     HLKAKGVSKVMLTAPAKGDVPNIVYGVNHDDGTVDYKTETIYSAASCTTNAIVPILYV
                     IENRLGIDKGHIETIHAYTNDQNLTDNYHKKPRRGRSAPMNMVLTSTGAASAAAKAIP
                     SLKGKLTANAVRVPIPNVSLAILSLTVQKQTTQQEVVEILRDAALNGRLVEQIRFSAS
                     TEAVSTDFIGDSCAGIFDAPATLVSADGKSIILYVWYDNEFGYTIQALRLAKHIAGVQ
                     WETYY"
     gene            871..2502
                     /locus_tag="PKZ74_13205"
     CDS             871..2502
                     /locus_tag="PKZ74_13205"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPM93759.1"
                     /translation="MPDTKYIFVTGGVSSSLGKGIISASLAKLLQARGFSVTIQKLDP
                     YINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVPTSQANNVTTGRIYKTVIE
                     KERRGDYLGETVQVIPHITDEIKRNIQILGNKYGYDFVITEIGGTVGDIESLPYIEAV
                     RQLKWEMGGHCLVVHLTLVPFLAAAGELKTKPTQHSVKTLLESGVQPDIIVCRTEKHL
                     NQNIRNKIALFCNVESTSVIESIDARTIYEVPLLMREEKLDAEVLRKLGIRPTQPPEM
                     GKWEEFIYRIRNPKYDVRICLVGKYVELKDAYKSIQESFIHAGAANYCKVHVQTIQSE
                     NLNETNAAEKLKGFDGILVAPGFGSRGIEGKIEAIRFARENNIPFLGICLGMQCAVIE
                     FARHVLGLEKAHSTEMDPDTPHPVIDMMEEQKTIQEKGGTMRLGVYDCRILPDTKVSE
                     IYPDTDFKERHRHRFEFNNRYLNDFEKNGMIAAGINPQRNLVEIMELRDHPWFIGVQF
                     HPEYRSTVAQPHPLFVKFIEAAVRQKQLRHPEEHS"
     gene            2569..>3673
                     /gene="yidC"
                     /locus_tag="PKZ74_13210"
     CDS             2569..>3673
                     /gene="yidC"
                     /locus_tag="PKZ74_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPM93760.1"
                     /translation="MNNSRNTIIGFLLIFAILIGYYFLTAPSKEELAEQRRRQDSVMA
                     LQRQKDSIALARQILADQQKATEPAQEVVADQITEPAKQLTGLVDRYGAFALASQGEN
                     KKYFLENDLIRIGLSSKGGRISYVELKNFKTWDGQPLILMDSDTNQFGFTFFSGNRTI
                     NTGNFYFQPEWIDGRRQTGDTLKVSGSEEARFAFRLYTSSDSIINKDQYIEYLFTLKG
                     DNYMLGFDVNFRNLTQTVDAARGYIDFEWSSDLRQQEKHTDQFNGATIYYKLKDDKVE
                     YLSETKDDNESLKDQIQWVSFKEQFFSSVLIADEHFADPVMQVSTLESHPSDRYLRSL
                     AYKIGVPYKGSPDESIGMSMYFGPNKYNILRKYK"
BASE COUNT         1069 a          875 c          870 g          859 t
ORIGIN      
        1 tgatcaatgg tcataaagtg aagatgcttt cagctaataa tccggaagat attgattatg
       61 aggcagaagg gattcataat gcactgctga tcgacagtac cggtgtgtgg accaagcgtg
      121 aagaccttgc ccggcacctg aaggccaaag gggtgagtaa agtgatgctg acggcgcccg
      181 ctaaaggaga cgtaccgaat atcgtttacg gtgtgaatca cgatgacggc acggtggatt
      241 ataagacaga aacgatctat tcagcagcat cctgcaccac caatgctata gtgcccattc
      301 tttacgtgat cgagaacaga ctgggcattg ataaaggcca tattgaaaca atccacgctt
      361 acaccaacga ccagaacctg accgataact accataaaaa accgagaaga ggccgttccg
      421 ctccaatgaa catggtgctt acctctacgg gcgccgcttc tgctgccgcc aaagccatcc
      481 cttcgctgaa aggaaagctg accgccaacg cggtcagggt cccgatccct aacgtttcac
      541 tggcaattct ctcattgaca gtgcaaaagc aaaccacaca acaggaagtg gtggagatac
      601 ttcgtgatgc cgcgctgaac ggaagactgg tcgaacaaat ccggttctca gcatcaaccg
      661 aagccgtgtc aactgatttt atcggagata gctgcgccgg gatttttgat gcccccgcta
      721 cgctggtttc agccgacggg aaatcgatca tcctgtacgt ttggtatgac aacgagtttg
      781 gctacacgat ccaggcactc cgtctggcta aacatattgc cggagtgcag tgggaaacct
      841 actattaaaa ttttagatca tataaataac atgccggata caaaatatat attcgttact
      901 ggtggcgttt catcatccct tggcaagggg atcatctcgg cttcgctggc aaaactgctg
      961 caagccaggg ggttctcagt cactattcag aaactggatc cttacattaa tgttgaccct
     1021 ggcaccctta atccctatga acacggggag tgttatgtta cggaagatgg tgcggaaacc
     1081 gacctggacc tgggccacta tgagcgattt ctgaatgtgc ccacatccca ggccaacaat
     1141 gtaacaaccg gcaggattta taaaaccgtc atcgaaaaag agcggagagg cgattacctc
     1201 ggagaaaccg tccaggtgat cccgcatatt acagacgaaa ttaagcggaa tatccagatc
     1261 ctgggcaata aatacggata tgattttgtg atcacagaga tcggtggaac agtcggcgat
     1321 atcgaatcat tgccttatat cgaagccgtc aggcagctga aatgggaaat gggtggccat
     1381 tgcctggtcg ttcacctcac cctggtccct tttctggctg ctgccggcga actcaagacc
     1441 aaaccgaccc agcactcggt caagacgctg ctcgaatccg gcgtacagcc ggatattatc
     1501 gtgtgccgga ccgaaaaaca tctgaaccag aacatccgga ataaaattgc cctgttttgt
     1561 aatgtcgagt ctacttcggt catcgaatcc attgacgcca ggacgatcta tgaggtgccg
     1621 cttctgatgc gggaagaaaa actggatgcg gaagtgctgc gaaagctggg aatacggcca
     1681 acacaaccgc ccgagatggg gaagtgggag gagttcattt accgtatcag gaacccaaag
     1741 tacgatgtca gaatctgcct tgtcggtaaa tacgtcgaac tgaaagatgc ctacaaatcg
     1801 atccaggaat cgtttatcca tgccggggcg gcaaattact gcaaagtgca tgtccagaca
     1861 atccaatccg aaaacctgaa tgaaaccaat gcggctgaaa aactgaaagg attcgacggg
     1921 atactggtag cgccgggatt tggttcccgg ggaatagaag gaaagataga agcgatccgt
     1981 tttgcacgcg aaaacaatat ccctttcctg ggtatctgcc tgggaatgca gtgtgccgtt
     2041 atagaatttg ccaggcatgt cctgggtctg gaaaaagccc attcaacgga aatggatccg
     2101 gatacgcctc atcccgtcat cgacatgatg gaggaacaga aaaccatcca ggaaaaaggc
     2161 ggcaccatgc gtttgggtgt atacgactgc cgtatcttgc ccgatacaaa agtcagtgaa
     2221 atttatccgg atactgattt caaggagagg cacaggcacc gctttgaatt taataaccgc
     2281 tatctcaatg acttcgagaa gaatggcatg atcgctgccg gtatcaatcc acagagaaac
     2341 ctggtggaaa ttatggagct aagagatcat ccatggttta ttggcgtaca gtttcatccg
     2401 gaataccgca gcacagtagc acagcctcac cctttattcg tgaaattcat cgaagccgct
     2461 gtccgtcaaa aacaacttcg tcatccggaa gagcattcat agttttttta atccctttga
     2521 ggcaattgat ttatctttgc cccactaaat aaaaaccagg aaaattgtat gaataattcc
     2581 agaaatacga tcataggatt tctactcatt tttgccatcc tgatcggata ttattttctt
     2641 accgcaccat ccaaagagga attagcagaa caaagacgcc ggcaggactc ggtcatggcc
     2701 ctccagcggc aaaaagattc cattgccctg gcccggcaga ttctggccga tcagcaaaaa
     2761 gcaacggaac ccgcacagga agttgtcgcg gaccaaatta cagaaccggc gaagcaattg
     2821 acaggcctgg ttgaccgcta tggggctttt gcactggctt cacaaggtga gaacaaaaag
     2881 tattttctgg aaaatgacct gatcaggatt ggactttctt ccaaaggagg ccggatttct
     2941 tatgttgaac tcaaaaactt caaaacctgg gacggacaac cactcatcct gatggattcc
     3001 gacaccaacc agttcggatt taccttcttt agcggtaacc gcacaatcaa taccggtaat
     3061 ttctattttc agcccgaatg gattgatggc cggcgacaga caggagatac tcttaaagtc
     3121 agcggaagtg aagaggcccg gtttgccttc aggctttata catcctccga ctccatcatt
     3181 aataaagatc agtatattga gtacttgttc accctcaaag gcgataacta catgctcggc
     3241 tttgatgtga atttccgcaa cctcacccag acggtcgatg cagccagggg ttatattgat
     3301 tttgaatgga gttcagacct ccggcaacag gaaaaacata cagaccagtt caatggcgcg
     3361 actatttact ataaactgaa ggatgataaa gttgaatacc tgtcggaaac caaagatgat
     3421 aatgaatctt taaaggatca gatccaatgg gtgtcgttta aagaacaatt cttctcctca
     3481 gtattgattg ctgatgagca ttttgcagat cctgtcatgc aggtatccac actggaatca
     3541 catcccagcg atagatatct gcgttcatta gcctataaaa taggtgttcc atacaagggc
     3601 agccccgatg aatccatcgg tatgtcgatg tattttggcc ccaataaata caatatcctc
     3661 cggaaataca agc
//
LOCUS       DAOPNH010000314         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_30169_length_3663_cov_5.015521, whole genome shotgun sequence.
ACCESSION   DAOPNH010000314 DAOPNH010000000
VERSION     DAOPNH010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30169_length_3663_cov_5.015521"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKZ74_13215"
     CDS             <1..557
                     /locus_tag="PKZ74_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93761.1"
                     /translation="GTNLIEEVKWEYKFSSWSPYLKISYLYNEDQLVESDYYTYQNDW
                     TISAKDVAVLFDGRIDQVIMYNCLSGDCTDDYLFKIFYNSDRVSRIEEYYKFGSTWDT
                     TGLYQFRYDAHKNLTSERFTGEGEDNSIEYKYEAGPGNFLQIKIPGGGLINTGNYPVP
                     SCTYRNVTSLQTLRPFQPLARFHQ"
     gene            complement(502..2010)
                     /locus_tag="PKZ74_13220"
     CDS             complement(502..2010)
                     /locus_tag="PKZ74_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPM93762.1"
                     /translation="MKTRIKFQTTLTTLITLTILTTLTTLPLTAQIPVQIMISDARTA
                     AGNQLSRHGRVDRTLQMETREIRVNDTKMAYVFDLDPTGYLVIGASSGLPPVIAYSFE
                     SDFGQMDEQNPLYTLVKTDLASRWTFVRTPGYSSEGYYENRWESILNEVVLQKTAPLF
                     EQWPAAGDGWLKTNWTQNPPYNNFCPMDPVTGTRSYAGCPATAMAQIMNFHNTTNLTR
                     FDDTDDYYHNYAGRQYWIDDDAETVDFPSFPELNTYLDTLDAHYLAGTTLTDADKAAL
                     TFACGTAATQVYTSQGSGTFGVSQAHEAYLRFGCNSVALLDSDDADLFDRLKQNIKDT
                     LPAHLAVVNESWTTGHNVVVDGYNTDDYYHLNFGWGGSYNGWYLLPDEIPYELTVIEG
                     VVVDIMKKSPVGIDESADGSWQSAVRIYPNPATGLIFIELPANYFRFQAYPESKFQIS
                     IFGINGQKPVQYQNMEQSMAFDATGLAPGIYFVKVTDGSGLRVGKVAKFVKK"
     gene            2174..2902
                     /locus_tag="PKZ74_13225"
     CDS             2174..2902
                     /locus_tag="PKZ74_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93763.1"
                     /translation="MKRVIVISLSLLMIGPVLLSAQESQRKPATLTASEIFLAEGDQD
                     QNAGLPLTPHDASGNRDLNYPIDQIQPLAGAYMAQFYQTDLAQSFVPSADKICGAGIG
                     IYNFYNNTGDVTIMLYDNLPNAGGNLLASGTTGFSFGEGISLIWADVSWPEVSITPGN
                     TYFLVFTCTNPQPMVAGNVENPYPYGMVYANPGYFPFPSFDYTFRTYTCTDMSNTIPL
                     SNWAIYLAIALMGLAVIWVVIRRR"
     gene            complement(2959..>3663)
                     /locus_tag="PKZ74_13230"
     CDS             complement(2959..>3663)
                     /locus_tag="PKZ74_13230"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM93764.1"
                     /translation="ADGVTSDPFFALVPPDEQFLNNYIISAGTGNIPNNYLNITSPTA
                     NTGTVLVDGNPVVNWTPIPSTTFSGAQVSINNGIHTITSNLPIGLLVYGFGNYDSYGY
                     IGGQAFAAVATINALDLTPTTGTAGINNLRCWEAEVTDSNGDPVSGIRVDFTITGANP
                     GAGFAFTDANGIATYCYTGTNAGQDVIVATTGALADTAQFTWTDQSAPVPVSNWALFI
                     GLFLIIATVIWKFRRA"
BASE COUNT          933 a          854 c          905 g          971 t
ORIGIN      
        1 caggaacaaa ccttatcgaa gaggtcaaat gggaatacaa attcagttca tggtcaccct
       61 atctaaagat atcttacctg tataatgagg atcaacttgt agaatcggat tattacacct
      121 atcaaaatga ctggacgata agtgcaaaag atgttgcggt tcttttcgat ggcaggatcg
      181 atcaggttat tatgtataac tgcctctccg gtgattgtac tgatgattat ctgttcaaga
      241 ttttttataa cagcgatcgg gtatcaagaa ttgaagaata ttacaaattt ggttccacgt
      301 gggacacgac aggcttgtat cagttccgtt acgatgcgca taaaaatctc acttcagaaa
      361 gattcaccgg cgaaggcgag gataattcca ttgagtataa atatgaagca ggacctggaa
      421 atttcctgca aataaagatc cctggaggtg gcctgatcaa tacgggaaat tacccggttc
      481 cttcctgtac ctatagaaat gtcacttctt tacaaacttt gcgacctttc caacccttag
      541 cccgcttcca tcagtaactt taacaaaata gatccccgga gcgagccctg tggcatcaaa
      601 ggccattgat tgttccatat tctggtattg aacgggtttt tggccgttta taccgaaaat
      661 ggagatttga aatttggact ccggatatgc ctggaacctg aaataatttg ctggcagttc
      721 gatgaagatc aacccggtag ccggattggg ataaatcctg actgccgact gccaactgcc
      781 gtctgccgac tcatcaattc caaccggcga tttcttcata atatctacaa caacgccttc
      841 gatcacagtg agttcgtatg ggatttcatc tggtaacagg taccagccat tgtaactgcc
      901 gccccatccg aagttgagat ggtaataatc atcggtatta tagccatcga ccaccacatt
      961 gtggcccgtc gtccacgatt cgttaaccac ggccaggtgg gccggcaacg tatccttgat
     1021 attttgtttc aatcgatcaa acagatcggc atcatcggaa tcgagcaagg ccaccgaatt
     1081 gcaaccgaag cgcaggtagg cttcatgggc ctggctgacg ccaaaagtgc ccgaaccctg
     1141 cgatgtgtaa acctgagtcg ctgccgtacc acaggcaaaa gtcaatgctg ctttatcggc
     1201 atccgtcagg gtcgtcccgg caaggtaatg cgcatcaagg gtgtcgaggt aagtattcag
     1261 ttccggaaag gagggaaaat cgaccgtttc ggcatcatca tcaatccagt actgccgccc
     1321 ggcataattg tggtaatagt cgtcagtatc atcaaaccgg gtgagattgg tggtgttgtg
     1381 gaagttcatg atctgtgcca tggcggtagc cggacagccg gcatagcttc gggtgccggt
     1441 aaccgggtcc atcgggcaga aattgttgta cggcggattc tgggtccagt tggtcttaag
     1501 ccatccgtct cccgcagccg gccattgctc gaaaagcgga gcggttttct gtaggaccac
     1561 ctcatttaat attgattccc agcgattttc atagtatcct tccgatgaat agccaggagt
     1621 acgtacaaat gtccaacggc ttgccagatc ggtcttcacc agggtgtaca acggattctg
     1681 ctcatccatt tgcccgaaat cactttcaaa ggaataggca atcaccggag gcagcccgga
     1741 agaggccccg ataaccaggt aaccggtggg atccaggtca aagacataag ccattttggt
     1801 atcgttcacc cggatttccc tggtctccat ctgaagtgtc cgatcaaccc ggccgtgtcg
     1861 ggaaagctga ttgccggcag cggtccgggc gtccgaaatc attatttgca ctggaatctg
     1921 tgctgttaag ggaagggttg taagggtggt aaggattgta agggtgataa gggttgtaag
     1981 ggtggtttgg aatttgattc ttgttttcat atcaggtgat gaaatttatt ttggtttggt
     2041 tttcgaatat acggttttca aagaagggtt tccaaccctt gagaacaaat ttatttgttt
     2101 ttgtgaagat tatatcttaa tttcgtcgat aagtttcctt gtcacaaatt aatcaaacct
     2161 taacaactta attatgaaga gagtaattgt tatttcactt tctttgctga tgatcggtcc
     2221 ggttttactc tcagcacagg aaagtcaaag aaaacccgct accttgacgg ccagtgagat
     2281 attccttgcc gaaggagatc aggaccagaa tgccggctta ccattgactc cgcatgatgc
     2341 aagcggcaac cgggatctta actatcctat cgaccagatt caaccgctgg caggcgcata
     2401 catggcccag ttctaccaga cagaccttgc ccaatcgttt gtcccctcag cagataaaat
     2461 ctgcggggcc ggcattggta tttacaattt ttataacaat acgggcgatg ttactatcat
     2521 gctttatgac aacctgccga atgccggggg aaatctgctg gcttcaggaa caaccggttt
     2581 ctcgtttggt gaaggtatat cgttaatctg ggccgacgta tcctggcctg aggtaagtat
     2641 cacaccggga aatacatatt ttttagtttt tacctgtaca aatccacagc ccatggttgc
     2701 cggaaacgtt gagaaccctt atccatatgg catggtatat gccaatccgg gctattttcc
     2761 gtttccatcc ttcgattaca ccttccggac ctatacctgt actgatatgt cgaataccat
     2821 tccactgagc aactgggcca tttacctggc cattgcgctg atgggactgg ctgttatctg
     2881 ggtcgtgatc aggagaagat aaaaaaaggc tgcccctcgc cggatgcagc ctctttgtgt
     2941 attaagaagg gaattctgtt atgctcttct gaatttccag atcaccgtgg cgatgatgag
     3001 gaaaagaccg atgaacaagg cccagttgct gaccggtacc ggcgccgact gatcggtcca
     3061 ggtgaactgg gccgtatcgg ccagggcgcc tgttgttgcg acgatcacat cctgacctgc
     3121 attggtacct gtataacaat aggttgcaat accgttcgca tcggtaaaag caaatcccgc
     3181 accgggattg gcacccgtta tcgtgaagtc gaccctgatt ccggataccg gatctccatt
     3241 gctatcggtc acctccgctt cccagcaccg caggttgttg atgcctgccg tgcctgtcgt
     3301 tggtgtcaga tccagcgcat tgatggtggc aacggctgca aaagcctgcc ctccgatata
     3361 gccgtatgaa tcataattgc caaacccata cacaagcaat ccgatcggaa ggttactggt
     3421 aatggtatgg atgccgttat tgatggacac ctgggcgccg gagaaagtag tactcggtat
     3481 gggggtccag ttgacaacgg ggttcccgtc gaccagaact gttcccgtat tggctgtagg
     3541 agaagtaatg ttgaggtagt tattggggat attccctgtc cctgctgaaa taatgtaatt
     3601 gttgaggaac tgttcatccg gtggtacgag ggcaaagaac ggatcagagg ttacgccgtc
     3661 ggc
//
LOCUS       DAOPNH010000315         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_30625_length_3622_cov_6.698626, whole genome shotgun sequence.
ACCESSION   DAOPNH010000315 DAOPNH010000000
VERSION     DAOPNH010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30625_length_3622_cov_6.698626"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PKZ74_13235"
     CDS             complement(<1..1118)
                     /locus_tag="PKZ74_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPM93765.1"
                     /translation="MANLDKVRKDLESYGVKDVAEIIHNPSYDYLYEAELDPVLEGYE
                     KGYLTESGAVNVLTGRFTGRSPKDKYIVLDEKTKDTVWWAAPNIRSDNKPIDHQTWEY
                     CYKLGAEQLSGKKLYVMDGYAGTNKDTRLNIRFVMEVAWQAHFVKNMFIRPTEEELEN
                     FKPDFVVLNAAKTINPEWKNMKRTDGVPFNSEVFVMFNLTEGRAVIGGSWYGGEMKKG
                     IFSVMNYYLPLKGVAAMHCSANVGKAGDTAIFFGLSGTGKTTLSTDPKRALIGDDEHG
                     WDDEGIFNFEGGCYAKCINLDKESEPDIYQAIKRDALLENLVVDANGKIDYKDGSITE
                     NTRVSYPIYHIDNIVKPVSKAGPAKKVIFLTADAYGVMP"
     gene            complement(1420..3622)
                     /gene="ppdK"
                     /locus_tag="PKZ74_13240"
                     /pseudo
     CDS             complement(1420..3622)
                     /gene="ppdK"
                     /locus_tag="PKZ74_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656792.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
BASE COUNT          765 a          974 c          860 g         1023 t
ORIGIN      
        1 ggcatgaccc cataggcatc agccgtcagg aaaatgactt tctttgccgg gcccgctttc
       61 gacaccggtt tgacgatgtt gtcgatatgg taaatagggt aggagacacg cgtattttcc
      121 gtgatggagc cgtctttata gtcgatcttt ccattggcat ccaccaccag gttttccagc
      181 agtgcatccc tttttattgc ctggtagata tcgggctcgc tctctttatc gaggttgata
      241 cattttgcat aacatccgcc ctcgaaattg aaaattccct catcatccca tccgtgctcg
      301 tcatcgccga tcagcgcccg tttcggatcg gtggaaaggg tcgtttttcc cgtacccgaa
      361 agtccgaaga aaatggccgt atcaccggct ttgcccacat tggccgagca gtgcatggcc
      421 gcaacgccct tcaggggcag ataatagttc atcaccgaaa aaataccctt cttcatttcg
      481 ccaccatacc agcttccccc gatcaccgct cttccttccg tcaggttgaa catcacaaaa
      541 acttccgaat tgaaaggaac accatcggta cgcttcatgt tcttccattc cgggttgatc
      601 gttttggctg catttaaaac tacgaaatcg ggcttaaaat tctccagttc ctcctcagtc
      661 gggcgaataa acatattttt cacaaaatgg gcctgccagg ctacctccat gacaaaccgg
      721 atattcagcc gggtatcttt gttggttcct gcataaccat ccattacgta aagctttttt
      781 cctgaaagct gctctgcgcc cagtttatag cagtattccc atgtctggtg atcaatgggt
      841 ttattatcgg aacggatgtt aggcgcggcc caccaaaccg tatctttcgt cttttcatcc
      901 agtacgatat acttatcttt aggcgagcgt ccggtaaatc ttcccgtcag tacattcaca
      961 gcgcctgatt ccgtcaggta acctttttca tagccttcca gaacaggatc aagttccgct
     1021 tcatacaagt aatcatagga aggattatga ataatttccg caacatcttt aacaccatac
     1081 gattcgaggt ctttcctgac cttatccaga ttagccattt cttcagatat tataaggtta
     1141 ataaagcagg caaaattagg aaatattcct gttacttttt ataccggtat tttggtgtat
     1201 gtattcttac gcagttaacc ggaaccaaag gaaccgaaac tgaaaaaagc cagtagccgg
     1261 cagacaatca ataaaaaaga ggatgctcat ttattaagac atcctcttca aacttatgat
     1321 tgagattcac tactattttt tcgctttctt tcctttttta gaagattttt cctcttcctt
     1381 ctttcctttc ttgtcggatt tctttgccag tttctcgcgg atcacggctt gagcagcggc
     1441 cagacgggcg attggaacac ggaaaggtga acaggaaaca tagttgaagc caagggaatc
     1501 gcagaattct acggaggatg gctcgccgcc atgctcgcca cagataccga tcttaatgtt
     1561 cgggcgggtc ttacggcctt tctttaccgc aatcttcatc agtttgccga caccgtcctg
     1621 gtcaagtacc tggaacggat cgtgcttcag gatgcctttc ttgaggtaca ccgggaggaa
     1681 ttttccggcg tcgtcgcgcg aatagccgaa tgtcatttgg gtcaggtcgt tcgtgccgaa
     1741 ggagaagaac tccgccgatt cggcgatttc atcggctgtc actgctgccc gcggcacttc
     1801 gatcatcgtt ccgaccaggt aatcgatccg gtctttcctt tcggcgaaaa ccttttcggc
     1861 cgtcgacctc acgatatctt cctgcatctt catctctttc agtgttcccg tcaatgggat
     1921 catgatctcc ggcttggcgt tcaccccttt tttcttcagg ttgagggccg cttcgataat
     1981 agccctggcc tgcatctcgg agatttcagg ataggtgttg ccaaggcggc atccgcggtg
     2041 acccagcatc gggttcattt cgtgcagttc atccactttc tgcttgatcg ctttgacgct
     2101 gacgcccatc tgcttggcca taaccttctg gttctcatct tcgtgcggta caaactcatg
     2161 cagtggcggg tcgagcaggc ggatagtcac cggcaggtcg tgcatagcct ccaggatacc
     2221 ctggaaatcg ccgcgttgga tcggaagcag cttgtcgagg gctttccggc gccctgcttc
     2281 atcatcagcg aggatcattt cacgcatggc gatgatccgg tcgccttcga agaacatgtg
     2341 ctcggtgcgg cagaggccga tgcccttggc gccgaagttt cgggcaacct gtgcatcatg
     2401 cggggtgtcg gcattggtgc ggacatgcat acgggcaaaa cggtcggcca gctccatcag
     2461 gtcgccgaaa tcgccgctga gctccgggtc ctgtgtggca atcttacctt cgtacacctc
     2521 gccggtcgat ccgttcagag agatccagtc gccttccttg aattcttttt tatcgatggt
     2581 catcgtgcgg ctcttgtaat cgatcttgat gcttccggca ccggagacgc agcatttacc
     2641 cattccgcgg gcaacgacgg cggcgtgtga ggtcatcccc ccgcgggccg taagaatgcc
     2701 attggcaacg ttcatccctt taaggtcttc aggagacgtt tctatacgca caagaatggt
     2761 ggattttcct tgagcagccc aggtttcggc ttcgtcggca aagaaaacca tctgaccggt
     2821 tgcggcgccc ggagaggcgg gaagtccttt tgccagcacc ttggcttttt tgatggcatc
     2881 tttatcaaaa acagggtgga gcagttcatc gagcttattg ggttcgatcc gcaacagggc
     2941 ggtttttgta tcgatcattt tctctttcag catatccatg gcaattttta ccatggcgga
     3001 gccggtacgc tttccgttgc gggtttgcag cagccacagc ttgccttcct ggatggtgaa
     3061 ctccaggtcc tgcatgtctt tatagtggtc ttccagtttt tgctggatct tgtctaattc
     3121 tacgtaaagc acaggcatcg attcttccag cgaagggaat ttcgaagcgc gggttttttc
     3181 agggacattg gccagctttg cccagcgcag tgaaccttct ttggtgatct gctgcggtgt
     3241 ccggataccg gcaaccacat cctcgccctg agcattgatc aggtattcgc cgttgaatag
     3301 gttctctccg gtacctgcat cgcgcgtaaa agcaacgccg gttgccgaat tttcgcccat
     3361 attaccgaaa accatagcct ggacgttcac ggctgtgccc cattcttcag ggatgttatt
     3421 gagcttccgg tagtaaatcg ccctgtcgtt catccagctg ctgaacacag cgacgatagc
     3481 cccccagagc tgttcccacg gatcggtggg gaagttcttt cctgtctctt ttttaacggc
     3541 tgctttgaac cttttcacca gttccttcag gtcgtcggcc gtcagttcgt tatccagctc
     3601 aactttcttc tcttttttca tc
//
LOCUS       DAOPNH010000316         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_31045_length_3588_cov_4.795358, whole genome shotgun sequence.
ACCESSION   DAOPNH010000316 DAOPNH010000000
VERSION     DAOPNH010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31045_length_3588_cov_4.795358"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..843)
                     /locus_tag="PKZ74_13245"
     CDS             complement(196..843)
                     /locus_tag="PKZ74_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPM93766.1"
                     /translation="MNHPLLKYNFIAIEGNIGAGKTTLATRLSDDYNARLILERFENN
                     SFLPKFYEQPDAFAFPLEMSFLADRYQQLKDKLAMPELFSTQTISDYFIDKSLIFARN
                     NLGSDEFNLYARLFQIISAFLPRPDLLVYLYLDIDGLLKNIKKRGRDYEQNIQPGYLE
                     QIQTGYLNHFRVLSGMRILIIDTGRVDFMENESDYRSFLEIINMDYEPGIHRILL"
     gene            complement(848..1342)
                     /gene="folK"
                     /locus_tag="PKZ74_13250"
     CDS             complement(848..1342)
                     /gene="folK"
                     /locus_tag="PKZ74_13250"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPM93767.1"
                     /translation="MNTVYLLLGGNMDDRAAWLEKAKDEIYLSIGKIVAQSSLYESEP
                     WGFQAEKCFLNQVVKTETDMEPAEILNEILSIENKLGRIRNNAETYQSRTIDIDILFY
                     NDQIVTEKNLVIPHPKIEERMFTLVPLAELDQSFVHPASRKTIGQLINECTDSISVYL
                     YHPD"
     gene            complement(1350..2363)
                     /gene="gap"
                     /locus_tag="PKZ74_13255"
     CDS             complement(1350..2363)
                     /gene="gap"
                     /locus_tag="PKZ74_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPM93768.1"
                     /translation="MTKIKVAINGFGRIGRITFRKLIENNRMEVVAVNDLADSATLAH
                     LLRYDSVHGRFPGTVINNGDHFLVNGSRILVYNVENQGDLPWNQLGIDVVIESTGRFV
                     SRQDAIRHVTEAGARKVIISAPTKTESDDVTYVVLGVNEEIIDRNEVIISNASCTTNN
                     VAPLIMILDRFWGIERAFITTVHSYTRDQNLIDAPHKDLRRARAAATSIVPTTTGAAK
                     AATRIFPHLKSNLGGAGIRVPVADGSLTDLTCTLRHPTTREEINWKFREAAQTYLAGI
                     LEYTEDPIVSVDIIGNTHSAVFDAGLTAVLGEKNKLVKVVAWYDNEMGYAARLVELVE
                     KFA"
     gene            complement(2375..>3588)
                     /locus_tag="PKZ74_13260"
     CDS             complement(2375..>3588)
                     /locus_tag="PKZ74_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93769.1"
                     /translation="VGEYSQLAANAGGGSENYTYTWTSNPPGFSSDIPDPVVQPSVNT
                     TYHVTVNDGYNSVQGQVLVHVNALPIPEAGENDTIWYGTIGKLYGSAIPGGSTYSYSW
                     EPADSLINPTAQNPTTYKLHSTNQFWLTVTDLNTGCVSADPDPVSVVVNGGPLAVTIS
                     TPDNLICLGDSTQLFAIPSGGNQVYHYNWSSVPPGMDDTTSAVTVSPTVNTTYKVEIF
                     DGFNYTHTEMEVSVAPAPIVTLGADRIVCPLDSLVLSSNAIGNFYWFNGSTDQSIIIG
                     STGIGFDIRTVWLNVENENGCIGTDTIQIVFDFTQCSGVDELDNNPYLYLYPNPSSGI
                     VYYECGGLGEYIGIEVSDLQGNRIYNGNIVALPGKIHKGSLSLEGFSKGIYLFKLISD
                     NDVMVRKVLLQ"
BASE COUNT          952 a          836 c          856 g          944 t
ORIGIN      
        1 aggaccagca ataccggtat gttatttgaa aatctaggga aagctatcca gatgaatgag
       61 gcctataaag ctgaagctca gatcgacagg gaatttgtta aatatttcac cgatcctaac
      121 tttaccagcc ttgtaaaata aggagatggt gtaaaacaca agaggctgcc ctttgaaagt
      181 ggcagcctct tttttttata gcaggatccg gtgtattccc ggctcgtagt ccatattgat
      241 gatttccagg aaggacctgt aatcggattc attctccatg aagtccaccc ttccggtgtc
      301 gatgataagg atcctcatcc cggataggac acgaaaatgg ttcaaatagc ccgtctggat
      361 ctgctcgagg tacccgggct gaatgttttg ttcataatcc ctcccccgct tcttgatatt
      421 tttcaggagg ccatcaatat ccaggtacaa ataaacaagc agatcgggtc ttggaagaaa
      481 tgcgctgatg atctggaaaa gacgggcata caggttaaat tcgtcggagc ccaaattatt
      541 tctggcgaaa atcaaagact tgtcgataaa ataatccgaa atagtctgtg tggagaatag
      601 ttccggcatg gccagtttat ccttcagttg ctgataacgg tcggcaagga aggacatctc
      661 cagcggaaag gcaaaagcgt ccggttgttc ataaaatttg ggcaggaagg agttattttc
      721 aaatcgttcc agtatcaggc gggcattata atcatctgac aaccgggtgg caagcgttgt
      781 cttgccggca ccgatattgc cttctatggc aatgaagttg tatttcagca atggatggtt
      841 catagcgtca atcgggatga taaagataga cagatatcga atccgtacat tcgttgatca
      901 gttgcccgat tgtcttccgg cttgcggggt gaacaaagga ttgatcaagt tcggccaggg
      961 gtacaagggt gaacatcctc tcctctatct ttgggtgtgg aataaccaga ttcttctcgg
     1021 taacaatctg gtcattataa aaaaggatat caatatcaat agtcctcgat tgatacgttt
     1081 cggcattatt ccggattcta cccagcttat tctcgataga taagatttcg ttaagaatct
     1141 cagccggctc catgtccgtt tccgttttaa cgacctggtt gagaaaacat ttctcagcct
     1201 gaaaacccca gggttcagac tcatagagtg acgattgggc tacaattttt ccgatcgaca
     1261 gataaatttc atccttcgcc ttttccagcc aggcagcccg gtcatccata tttcctccta
     1321 aaagcagata caccgtattc ataggcaggt caggcaaatt tttcaaccag ttctaccagc
     1381 ctggcggcgt atcccatttc gttatcgtac caggccacaa ctttgacaag cttatttttt
     1441 tctccaagta cagccgtaag gccggcatca aaaacggcag aatgtgtatt tccgataata
     1501 tcgactgata caatcggatc ttcggtgtac tccagtattc cggccagata tgtttgcgcc
     1561 gcctcccgga atttccaatt gatctcctcc cgtgtggttg gatgacgcaa ggtgcaggtc
     1621 agatcagtca gggagccgtc tgcgacaggc actcgaatgc cggctccgcc caggttggat
     1681 ttaaggtggg ggaagatgcg ggtagcggct ttggcagcgc cggtggttgt agggacaatt
     1741 gaagttgcgg cggccctggc ccggcgaaga tccttgtggg gcgcatcgat caggttctgg
     1801 tctcttgtat aggaatgcac cgtggtgata aatgcccgtt ctatccccca gaacctgtcg
     1861 agaatcataa taagcggtgc aacattgttt gtagtacagg atgcattgga aataatgacc
     1921 tcgttccggt cgataatctc ctcatttaca cccaacacaa cgtaggtaac atcatcgctt
     1981 tctgttttcg tcggagcgga tatgataacc tttcgtgcac ccgcttcggt cacatgccgt
     2041 atggcatcct gccgggagac aaaccggccg gtagactcaa tgaccacgtc gatacccaat
     2101 tgattccatg gcagatcgcc ttgattctca acattataga caagtatcct ggaaccattt
     2161 accaggaaat ggtctccatt gttaattacg gtaccgggga accgtccgtg gaccgaatca
     2221 tacctgagga gatgggccag ggtggcagaa tcagcaaggt cattgaccgc caccacttcc
     2281 atccggttat tctcgattaa tttcctgaag gtaatccttc caattcgccc aaacccatta
     2341 atcgcaacct taatttttgt catgaaaata ttacctactg caataaaact tttctgacca
     2401 tgacatcgtt gtcggagatc agcttaaata aataaattcc ttttgaaaat ccctccaaag
     2461 atagggaacc tttgtgaatt tttccgggta aagcgacaat attcccgttg taaatccggt
     2521 ttccctgcag gtcggaaact tcaattccga tatattctcc cagcccgcca cattcataat
     2581 aaacaattcc tgaagatggg ttgggataaa ggtagaggta agggttattg tcaagttcat
     2641 caacaccgga acactgggta aaatcaaaaa caatctggat ggtatcggtt ccaatacaac
     2701 cattttcatt ttcgacattc agccagaccg tccggatatc aaaaccgatc ccggtactgc
     2761 ctattataat ggattgatca gtggatccat taaaccaata aaaattccca atggcattgg
     2821 aggataaaac caatgaatca agcgggcata caatccggtc tgctcccagg gtcacaatgg
     2881 gagccggtgc tacacttact tccatttccg tatgagtata attgaacccg tcaaagatct
     2941 ccactttgta ggtcgtatta acagtcggag agacagtgac ggctgaagtc gtatcatcca
     3001 tgccgggagg aacggaactc cagttgtaat ggtacacctg gtttccgcct gacgggatgg
     3061 caaataattg ggtagaatcg ccgaggcaaa tgaggttatc gggagtcgaa atagttacag
     3121 ccagcggccc gccgttaaca acaacactca ccggatcggg atctgccgac acgcaacctg
     3181 tgttcaggtc ggtaactgtc agccagaact ggtttgtgga atggagttta taagttgtcg
     3241 ggttctgcgc ggtcgggttt atcagagaat cggcaggctc ccaggaataa gaataggtgc
     3301 ttccgcccgg aatggctgat ccgtaaagtt tgccgatagt accataccag atcgtgtcgt
     3361 tttctccggc ctccgggatc gggagggcgt tcacatgtac gagtacctgg ccctgaacag
     3421 aattgtatcc gtcgttgacc gtcacatggt aagttgtgtt gacactgggt tgaacaacag
     3481 gatcaggaat atctgagctg aatcccgggg gatttgaggt ccacgtatat gtatagttct
     3541 cggagcctcc gccggcattg gccgccagtt gcgaatattc gcccacac
//
LOCUS       DAOPNH010000317         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_31063_length_3586_cov_6.788162, whole genome shotgun sequence.
ACCESSION   DAOPNH010000317 DAOPNH010000000
VERSION     DAOPNH010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31063_length_3586_cov_6.788162"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKZ74_13265"
     CDS             <1..680
                     /locus_tag="PKZ74_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPM93770.1"
                     /translation="FVNEDSAYGTGQLPDKDGQMYAIGLDKLYAIPTAEVPVTNVYRD
                     VILKADDLPVKNVAYSSCFRREAGSYGKDVRGLNRLHQFDKVEIVQISHPDLSYAQLE
                     EMRLHVASLLQKLELPFRIVRLCGGDMSFTSALTYDFEVWSGAQKRWLEVSSVSNFEN
                     FQANRMKLRFKDEEGKTRLAHTLNGSALALPRVVAALLENNQADDGIRIPAALQKFTG
                     FEKIILK"
     gene            658..2559
                     /locus_tag="PKZ74_13270"
     CDS             658..2559
                     /locus_tag="PKZ74_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93771.1"
                     /translation="MKKSSLNKFYKTLFPLVVIAFITAMAPLNTFSQVFPEVSTPEAS
                     KKQQEQEKEKLAFQYYGNKEYEKALELFRDLYGIQPTYHRYTYYFYCMTYLNLFDEAE
                     KVVKGRMKAEPESYRYQVDLGYLYLLTDKPEKATKIFDEVLAGMKPTKAAVNELANAF
                     VMRQLYDYTIKVYLKGREILGYTEMFHQDLARIYEVSGNYSGMIEEYLDLLQADPMQL
                     ETVQNRLQNALNKDIDDKISNLLRESLLRRNQRNPDNRNYGEMLLWLSVQQKDFEFAL
                     IQARSLDTRFNEGGFLVLEVGDLALSNKEYDVAIDAFSYITRKGQMHPLYAECLTGSL
                     QARFLRLTEGYGSGEDNLEDLEKEYLTAIELLGKNARTLILMRDLSHLQAFYLNKLDD
                     AVALLEEILSIPNAPDLVKADIKLELGDIYLFVGEVWEASLLYSQVEKMFKNDPKGHE
                     AKFRNARLSYYIGEFDWAKAQLDVLKAATSKLIANDAMDLSLLIGDNMDADSTYTTLS
                     CFARAELLKYRNQDSLAVITLDSIAMMGLYNPLDDEVLFSKAEIFIRQKKFDLADSLL
                     ATLVKVYSRDILADNALFKRAEMQAEVFGNDPLALELYQQLMLDYPGSLFATEARKRF
                     RQLRGDFNN"
     gene            2571..3341
                     /gene="rsmA"
                     /locus_tag="PKZ74_13275"
     CDS             2571..3341
                     /gene="rsmA"
                     /locus_tag="PKZ74_13275"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPM93772.1"
                     /translation="MNNFVRPKKHLGQHFLKDENIARKIVGSRQSAVGSLLEIGPGMG
                     VLTKYLLDLPGIECYFIDTDREAIDYLNNAFPGHEARFIHGDYLRMNTAKIFTGSYAV
                     IGNFPYNISSQIFFRILEERQNVTEVVGMVQKEVAERLAAGPGSKTYGILSVLLQAYY
                     RIEYLFTVNENVFVPPPRVKSAVIRLTRNDTERLDCDEELFFRVVKTAFNQRRKTLRN
                     SLKSFYIDYERGKILHLAGKRAEQLSVEDFVRVTRVAV"
     gene            complement(3376..>3586)
                     /locus_tag="PKZ74_13280"
     CDS             complement(3376..>3586)
                     /locus_tag="PKZ74_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPM93773.1"
                     /translation="PGQGRVVLSVYDIRGELVRDLYIGKQSPGIYTIEWNGLNNAGAP
                     VSPGIYFCRLQTDGYSETIKMFLLK"
BASE COUNT          945 a          872 c          928 g          841 t
ORIGIN      
        1 tcttcgtcaa tgaggattcg gcctacggca ccggccagtt gcccgacaaa gacgggcaga
       61 tgtacgccat cgggctggac aagctttacg cgatcccgac agccgaagta cctgttacga
      121 atgtgtaccg cgatgtcatc ctgaaagccg acgacctgcc ggtgaagaat gtcgcctatt
      181 cgagctgttt ccgtcgggaa gccggatcct acggcaaaga tgtcagggga ttgaaccggc
      241 tgcaccagtt cgataaagtc gaaattgtac agatatcgca tcccgatctc tcctacgccc
      301 aactcgagga gatgcggctt catgtcgcat cgcttctgca aaagctcgag ctgccgttcc
      361 ggattgtcag gctttgcggg ggcgatatga gctttacctc tgcactgacc tatgatttcg
      421 aagtatggtc gggcgcgcaa aagcgatggc tcgaagtgag ctcggtgtca aacttcgaga
      481 atttccaggc caaccggatg aagctcaggt ttaaagatga agaagggaaa acacggctgg
      541 cccacacact aaacggcagc gcccttgcgt tacccagagt ggtcgcagca ttgctggaaa
      601 acaaccaggc ggatgacggg atcaggattc cggcggccct gcaaaaattc accggttttg
      661 aaaaaatcat ccttaaataa attctataaa accttattcc ctttagtggt catcgcattc
      721 ataacggcaa tggccccact gaatacgttt tcccaggttt ttccggaggt cagtaccccc
      781 gaggcttcta aaaaacagca ggaacaggaa aaagagaagc tcgctttcca gtattatgga
      841 aacaaggaat atgaaaaagc gctcgaatta ttccgtgatc tctacggtat tcagccaacc
      901 tatcaccggt atacctatta tttctattgc atgacttacc tcaacctgtt tgatgaagcg
      961 gaaaaggtgg tcaaaggaag gatgaaagcc gaacccgaat cgtaccgtta ccaggtggat
     1021 ctcggttatc tatatctcct gacggataaa ccggagaaag caactaaaat atttgacgag
     1081 gtactggccg gcatgaaacc gaccaaagca gcggttaatg aactggccaa tgcttttgtg
     1141 atgaggcagt tatacgatta tacgataaag gtttatctca aagggcggga aatactgggc
     1201 tacaccgaaa tgttccacca ggacctggcc aggatctatg aagtcagcgg caattacagc
     1261 gggatgattg aagaatacct ggatctcctg caagccgatc ccatgcaact ggaaacggtt
     1321 caaaacaggc tgcagaatgc actgaacaaa gacatcgacg acaagatcag caacctgctt
     1381 cgggaatcgc tgctgagaag aaaccagcgt aatcccgaca accgcaatta cggcgaaatg
     1441 ctgctctggc tttccgttca gcagaaagac ttcgaatttg cgctgatcca ggcccggtcg
     1501 ctcgatacgc gcttcaatga aggcggtttc ctggtgctgg aggtaggcga cctggcgctg
     1561 tcgaacaaag aatatgacgt ggcgattgac gcattctcct acattacccg caaaggtcag
     1621 atgcatccgc tgtatgccga atgcctgacc ggatcgctcc aggccaggtt cctgagactg
     1681 accgaaggtt acggaagcgg ggaagataac ctggaagacc ttgaaaaaga atatctgaca
     1741 gccatcgaac ttctcggcaa gaatgcccgt accttgatcc tgatgcgtga cctgtcgcat
     1801 ctgcaggcct tctacctcaa caagctcgat gatgcggtag cactgctcga agagattctc
     1861 agcatcccga atgcccccga cctggtaaag gccgacatca agctggaact cggcgatatc
     1921 tacctgttcg tcggggaggt gtgggaagca tcgttgcttt attcacaggt agaaaaaatg
     1981 ttcaagaacg atccgaaagg gcatgaagcc aagttcagga atgcccgcct gtcgtactat
     2041 ataggcgaat tcgactgggc taaagcgcag ctcgatgtgc tgaaagccgc cacatccaag
     2101 ctgatcgcca acgatgcaat ggatttgtcg ttgctgatcg gcgataatat ggatgccgac
     2161 agcacttaca ccaccctttc ctgctttgcg cgggccgaac tgctaaaata ccgcaaccag
     2221 gacagcctgg cggtaatcac cctcgattcc attgccatga tggggcttta caatcccctg
     2281 gatgatgagg ttttattcag caaggcagaa atctttatcc ggcagaagaa atttgatctg
     2341 gccgactcac tgctggctac ccttgtcaaa gtatattcga gggatatcct ggccgacaat
     2401 gcgctgttta aaagagcgga gatgcaagcg gaagtattcg ggaatgatcc gcttgccctg
     2461 gagctttacc agcagcttat gctcgattat cccggcagtc ttttcgccac cgaagccaga
     2521 aaacgtttcc ggcagctgcg cggcgatttt aataattaag aactttttgt gtgaataatt
     2581 ttgtcaggcc gaaaaagcac ctggggcagc attttctgaa agatgagaat attgccagga
     2641 aaatagtcgg cagtcggcag tcggcagtcg gcagtttgct tgagatcggg ccgggaatgg
     2701 gggtgttgac gaagtacctg ctggatttgc cgggaattga atgctatttc atcgatacgg
     2761 acagggaggc gatcgattat ttgaataacg cgtttcccgg tcatgaggcg cgattcattc
     2821 acggtgatta tctcaggatg aacacagcga agatttttac agggtcttat gctgttatcg
     2881 ggaattttcc atacaatatt tcgagccaga tatttttccg gatcctggaa gagaggcaga
     2941 acgtaacaga ggttgtcgga atggttcaga aagaggttgc ggagaggctt gccgctggac
     3001 cggggtcgaa aacctacggc atcctgagcg tattgctcca ggcctattac cggatcgaat
     3061 acctgtttac ggtgaacgaa aacgttttcg tcccgccacc cagggtcaaa tcggctgtga
     3121 tacggcttac caggaacgat acggaaagac ttgactgcga tgaagagttg ttcttccgcg
     3181 tggtcaagac cgctttcaac cagcgccgga aaacgctccg gaattcttta aagtcgtttt
     3241 atatcgatta cgaacgcggt aaaatccttc acctggcggg caaaagggca gaacagctca
     3301 gtgtggagga ttttgtgagg gtgacgaggg tagcggtgtg attcatgttc cgttttgcat
     3361 tttacatttt tcattttatt tcaaaaggaa cattttaatc gtttccgaat acccatccgt
     3421 ctgcagcctg cagaaataaa tccccgggct caccggcgct ccggcattgt tcaagccgtt
     3481 ccattcaatg gtataaatgc ctggagattg tttcccgatg tagagatccc tgaccagttc
     3541 tcccctgata tcatatactg acaggacaac cctgccttgt cccggc
//
LOCUS       DAOPNH010000318         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_31338_length_3562_cov_8.316510, whole genome shotgun sequence.
ACCESSION   DAOPNH010000318 DAOPNH010000000
VERSION     DAOPNH010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31338_length_3562_cov_8.316510"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKZ74_13285"
     CDS             complement(<1..639)
                     /locus_tag="PKZ74_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPM93774.1"
                     /translation="MSTSTPPEDKPGKSLNFIELIIEEDIKNNKNDGRVLTRFPPEPN
                     GYLHIGHAKSICLNFGLGMKYGGKTNLRFDDTNPTKEEQEYVDSIMDNVRWLGFDWEE
                     RLFFASDYFDQLYEWAVMLIKEGKAYVDDHSAEEVSENRGTPTQPGLESPFRNRSVEE
                     NLDLFERMKNGEFPDGTRILRAKIDMASPNMHMRDPVLYRILHAHHHRTGDKW"
     gene            complement(760..1182)
                     /locus_tag="PKZ74_13290"
     CDS             complement(760..1182)
                     /locus_tag="PKZ74_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93775.1"
                     /translation="MKPQAENDDLIRNLVRRKGIEKAPDHFTDKVMERINSPSLADDS
                     PLISRGTLLAIIVGVAAMIVVILTVDIPYFDNLFSSDRIQKISMNIFTDGFFRTMASF
                     FREFSSITWVVIGAALGLVLIDRILRKRFSETKGMMIW"
     gene            complement(1194..1757)
                     /locus_tag="PKZ74_13295"
     CDS             complement(1194..1757)
                     /locus_tag="PKZ74_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM93776.1"
                     /translation="MDDNHYINQVIAGNSALFAVLIDRHKDLVFTLCLNITRSREDAE
                     EVAQDSFLKAFQKLSTFRNDSGFRTWLYRIAYNEAITKVRKKKVVTTSLDDRFIDELP
                     DEEYEESIAGMDEAEQKEVIGRVLEKLPETDRVIVTLYYLESCPVREISEITGLGESN
                     VKVRLHRIRKRIYSELQHIFKIKSLSL"
     gene            2036..2365
                     /locus_tag="PKZ74_13300"
     CDS             2036..2365
                     /locus_tag="PKZ74_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93777.1"
                     /translation="MLELTPIFVLTIIFGFIYAIIYLNIRRKERMALLEKGADPSIFQ
                     EPKTEKQTSLRYGLFLIGLAIGILMGNVLEVSTELGPEASYFSMVFLFGGIALVISHF
                     LGRKVQS"
     gene            complement(2362..2916)
                     /gene="thpR"
                     /locus_tag="PKZ74_13305"
     CDS             complement(2362..2916)
                     /gene="thpR"
                     /locus_tag="PKZ74_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPM93778.1"
                     /translation="MKRLFAAVKIHPSPEFLKTYNSLKAGLRFCRITWVKPESIHVTL
                     KFFGETEEKKIPEISRVLKEAASRHEPFSCELVNVGIFGSSYSPKVIWFGIDQSEPLK
                     KLGTDVLQSVEKIGWEPDRQNFVPHLTIGRIKDVPDKQLFQKMIDMHKTDRLQEFSVS
                     EFNLYESILLKDGPLYKVVESYRL"
     gene            3041..>3562
                     /locus_tag="PKZ74_13310"
     CDS             3041..>3562
                     /locus_tag="PKZ74_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93779.1"
                     /translation="MFYRIGLAFFLISAFFLVRAQDTTYLRYSLTITKEELSAHVYKL
                     ASEEFSGRYTGSKGEYEAGKYILEEFAEDGLQAPVINGKVTYRQVFALDNCRWKDQRL
                     MVNGGELKVGRDFLFLSDPVDIKGNFPVIFAGFGIEDKAYSDFSGLDVKGKILLVFSG
                     EPRDKKGRSVITGK"
BASE COUNT          882 a          834 c          863 g          983 t
ORIGIN      
        1 ccatttgtcg ccggtgcggt ggtgatgggc atgcaggatg cggtacaaca ccgggtcgcg
       61 catgtgcatg ttgggcgaag ccatatcgat cttagccctg agaatgcgtg tgccatccgg
      121 gaattcgcca ttcttcatcc tttcaaacag gtccaggttc tcttctaccg aacggttccg
      181 gaaagggctt tctaagccgg gctgggtagg tgttccgcgg ttctcgctga cctcctcggc
      241 gctgtggtca tccacatagg ctttgccctc tttgatgagc atgacagccc attcatagag
      301 ctggtcgaag tagtcggatg cgaagaacag ccgttcttcc cagtcgaacc cgagccagcg
      361 gacattgtcc atgatcgagt cgacatattc ctgctcttct ttggtcgggt tggtatcgtc
      421 gaagcggagg ttggtcttgc cgccgtattt catgccgagg ccaaaattca ggcagatcga
      481 tttggcatgg ccgatatgaa ggtagccgtt gggctcggga gggaaacggg tgaggacgcg
      541 gccgtcgttt ttgttgttct ttatgtcttc ttcaatgatc agctcaatga aattgagtga
      601 tttaccgggt ttgtcttcgg gaggagtgct ggttgacatg gattcgctga ttttcagtaa
      661 taagggtgca aaattaaaat attttagggc gcgaccgggt atttttcagc gaaaaagcgg
      721 attaataggt atatgagaaa taaagcggat tgtcagatct caccagatca tcatgccttt
      781 ggtttccgag aagcgcttgc gcaggatccg gtcgatcagc accaatccca gcgctgcgcc
      841 gatcaccacc caggtaatgc tcgaaaactc acggaaaaaa gaggccatcg tcctgaaaaa
      901 tccatcagta aaaatattca tcgatatttt ctgtatcctg tcggaagaga agagattatc
      961 aaaataaggt atatccaccg taagaatcac cacgatcatg gccgcaaccc ccacgatgat
     1021 cgccagcaac gtcccccggg agatcaacgg cgagtcatcc gccagtgaag gagaattgat
     1081 cctctccatc actttgtctg taaaatgatc cggggccttt tcaatgcctt tgcggcgaac
     1141 aaggttccgg atgagatcgt cattttcagc ctggggtttc atatcaacag attttatagg
     1201 gataacgatt tgattttaaa aatatgctgc aattctgaat aaattctttt tctgatccgg
     1261 tggagcctca ctttcacatt gctttcgccc agcccggtaa tttccgagat ctccctgaca
     1321 gggcaacttt ccaggtaata cagcgtcacg atcacccggt cggtttcagg cagcttttcc
     1381 agaacccgtc caataacctc tttttgctcc gcttcatcca tccctgcaat gctttcctcg
     1441 tattcttcat ccggcaattc atcgatgaac cggtcgtcca ggctggtcgt caccaccttc
     1501 ttcttcctga ctttcgtgat cgcttcgttg taagcgatcc ggtaaagcca tgtccggaat
     1561 cccgaatcgt tccggaaggt gcttagcttc tgaaaggctt tcaggaaaga atcctgtgcc
     1621 acctcttcag catcttcccg gctgcgtgtt atattcaggc acaacgtgaa cacaaggtcc
     1681 ttgtgcctgt cgatcaacac agcgaacaac gcgctgttgc cggcgattac ctggttgata
     1741 taatggttgt cgtccatcgg gattaactgt tcattggacg gtgaagtgta gtaaaggtta
     1801 caaagttaaa tcgaaaccag gtaaattctt tataggttcc gtgaaatctg tgcccttaat
     1861 ctgtgttaat ctgtgagaaa tctactggtt ctcacagatt aacacagatt gaaaacacag
     1921 aaaggcacag atttaaattg cgatttttac caatagtgta acctctcccg aattcatgcc
     1981 gtcaaatgtt caaatcgtca atcaaataaa aattaagaat taaaaattta aaattatgtt
     2041 agaactcact ccaatcttcg tcctgaccat catctttggt ttcatttatg cgatcattta
     2101 cctgaatatc cgcaggaaag aacggatggc actgctggaa aaaggtgcag atccttccat
     2161 cttccaggaa cccaaaacgg aaaaacagac atcgctccgc tatggtttat tcctcatcgg
     2221 gctggccatc ggaatcctga tgggcaacgt gctggaggtc agcaccgagc tgggccccga
     2281 agcctcttac ttctccatgg tcttcttatt tggcggcatc gcactggtga tcagccactt
     2341 cctggggagg aaagttcaaa gttaaagtct gtagctctcc acaaccttgt aaagaggccc
     2401 gtctttcagc aatatgctct catacaaatt aaactccgac acgctgaact cctgcagcct
     2461 gtcggttttg tgcatgtcaa tcatcttctg aaataattgc ttgtcaggca catcttttat
     2521 tcttcctatg gtaagatgcg gaacaaaatt ctgcctgtcg ggctcccagc ctattttctc
     2581 gaccgactgt aagacgtctg ttcccagttt tttgagtggt tccgactggt ctattccaaa
     2641 ccagatgact ttagggctgt aggagctgcc aaagattcca acattcacca gttcgcagct
     2701 aaatggttcg tgccgggagg ctgcttcttt caataccctg ctgatctccg gtattttctt
     2761 ctcctccgtc tctccgaaga acttcagcgt aacatggata ctttcgggct tcacccaggt
     2821 gatccggcag aatctcaacc ccgctttcag actgttatag gtctttaaga actccggtga
     2881 aggatggatc tttacggcgg caaagaggcg tttcataacg ttagcaattt ctggtaaaag
     2941 tagttaattg atgatcacag tttcatttga aacactttgc atgggagggg attaagggga
     3001 ggggaaagaa taattaaatt tgaagaataa ttgtacgagt atgttttacc gaataggatt
     3061 agctttcttc ttaattagtg cttttttcct ggttcgagcc caagatacca cttatcttcg
     3121 gtattccctg acaatcacca aggaagagct ttccgctcac gtttacaaac tggcttctga
     3181 agaattcagc ggccggtaca ccgggagcaa aggagagtat gaagccggga aatatatttt
     3241 ggaggagttt gcggaggatg ggttgcaggc cccggtcatc aatggaaagg ttacgtacag
     3301 gcaggtgttt gcgctcgata actgccggtg gaaggatcag aggttgatgg tcaatggcgg
     3361 ggagctgaaa gtcgggcgtg atttcctttt tttgagcgat ccggtggata tcaaaggcaa
     3421 tttcccggtc atttttgccg gatttggcat agaagacaaa gcctattccg atttcagcgg
     3481 cctggatgta aaagggaaga tcctgctggt tttttccgga gagccgaggg ataagaaggg
     3541 ccggtcggtc ataaccggga aa
//
LOCUS       DAOPNH010000319         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_31845_length_3521_cov_7.718119, whole genome shotgun sequence.
ACCESSION   DAOPNH010000319 DAOPNH010000000
VERSION     DAOPNH010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31845_length_3521_cov_7.718119"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PKZ74_13315"
     CDS             <1..1585
                     /locus_tag="PKZ74_13315"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM93780.1"
                     /translation="AVAAQYDITSLVDYVILNSYVVCSDWMNWNVGWWRGLDPEGSHR
                     KWAYILWDEDATFGHYINYTGIPAQSPYVSPCFPEDLTDPWQDPEGHIAVLNKLRTND
                     EFNQYYISRYIDLLNTTFTNDYMLNLLDSMAAIIAPEMPQHTQRWGGSVTQWQNNVQK
                     IRNFITTRNSIMDAGLRNCWDLTGPYDLVIDAEPEGVGKVQINSLELNSFPWDGDYFG
                     GVDVKLSAIETSPYFEFDRWVLKNHEVTPSDTMKDVRLSLIMGDSVTALFKPRVFEDS
                     LVINEINYNSDGGFDPGDWVEFYNPHPYPLDISGWVFKDEDDAHEYEFPAGTVIGQEG
                     YLVLAGDTAAFDSLFPDVDNYLGPMGFGLSGNGELIRLYDNEGTLIDTVLYDDEDPWP
                     TEPDGDGATLELINPAWDNALAESWAASELYGTPGKENGNYVKVPDPVATPDISCQVI
                     PNPFRSNAIFRIRSEEILSNASIEVFDLYGRKVKEINGIQSNYIPLSGQGLNAGIYIY
                     RILDSKGMVLFQGKFVMTE"
     gene            complement(1639..1899)
                     /locus_tag="PKZ74_13320"
     CDS             complement(1639..1899)
                     /locus_tag="PKZ74_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPM93781.1"
                     /translation="MKCPTCNVPLVMSERQGIEIDYCPQCRGVWLDRGELDKIIERSM
                     NEDQPKGFNQPPPQQRGHHDDYRHHGDYYKGRKKGWLGDLFD"
     gene            complement(1945..2520)
                     /locus_tag="PKZ74_13325"
     CDS             complement(1945..2520)
                     /locus_tag="PKZ74_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93782.1"
                     /translation="MNDRFSAPNVPEKNVNNANGLGGLIEELKAADQKGKRLVSRFMI
                     IFFVLLGVYAIQIATKNGPLSIGYELLAAGFIFILVYFFRKIILIRRIDYSAPVIIFL
                     RKAEKRYTFITAADWFIIIPILGVIGTGGGYIVYYSFIKYFEDSPWPLVIYIIFFVSV
                     IIFGFWAGKKDWKKENGVILDKIKKSIDENR"
     gene            complement(2513..3049)
                     /locus_tag="PKZ74_13330"
     CDS             complement(2513..3049)
                     /locus_tag="PKZ74_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM93783.1"
                     /translation="MEKEVLFRQIADENKGRIFRICRYFFQDREDCNDACQEALIRIW
                     QNLSTFRNQSQLSTWIYRVTVNTCLTFIRKDKSRKTLFELSGIRSAENRPDEPIIEEN
                     PQEEYKLHFFRRFMEELSPVDRTAVSLYLEELSTREIADVTGLSESNVRVKIHRLKEK
                     IKIKWKEEQNGTGRIQNE"
     gene            complement(3178..>3521)
                     /locus_tag="PKZ74_13335"
     CDS             complement(3178..>3521)
                     /locus_tag="PKZ74_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPM93784.1"
                     /translation="PVETLHATPVETLHATPVETLHATPVETLHATPVETLHATSLPG
                     LLDQAMRRRNPYYNDLIRGGILEPLHIVPLPAGTFKRYLESQGKLGGQNKVVHVCDDR
                     RIAEALMDLAI"
BASE COUNT          908 a          835 c          801 g          977 t
ORIGIN      
        1 cgcagtagct gcacaatacg acatcaccag cctggtggat tatgtaatac ttaactccta
       61 tgtggtgtgt tccgactgga tgaactggaa tgtaggctgg tggcgcggat tggatccgga
      121 agggagtcac cggaaatggg cgtatatcct ctgggatgaa gatgccacct tcgggcatta
      181 catcaattat accggtattc cggcccaatc accgtatgtt tcaccctgtt ttcccgaaga
      241 ccttaccgat ccgtggcagg acccggaggg gcatattgca gtgctgaaca aactgcgcac
      301 caacgacgag tttaaccagt actatatttc ccggtacatc gaccttctca acaccacatt
      361 taccaatgat tacatgctga acctgctcga cagtatggcg gcgatcatcg cccctgaaat
      421 gccccagcat acgcagcggt ggggaggctc ggttacccaa tggcagaata acgtacagaa
      481 gatcaggaat ttcatcacta cccggaacag tattatggac gccggcctga gaaactgctg
      541 ggacctgacc gggccttatg atttggtcat tgatgccgag ccggaaggtg tcggcaaagt
      601 gcagatcaac tcgctggagt tgaattcttt cccatgggac ggcgactatt ttggcggtgt
      661 cgatgtaaaa ttgtcggcaa tcgaaaccag cccctatttt gaattcgacc ggtgggtctt
      721 aaagaatcat gaagtaacac cttcggatac catgaaggat gtcaggctga gcctgattat
      781 gggcgattcg gtgactgctt tgttcaagcc cagggtattc gaggattccc tggtgatcaa
      841 cgaaattaat tataactctg acggaggatt tgatccgggc gactgggtgg agttttataa
      901 tcctcatcct tatccgcttg atatttccgg gtgggttttt aaagatgaag atgatgcgca
      961 tgaatatgaa ttcccagccg gaaccgttat cggtcaggag ggatatctgg ttttggcggg
     1021 cgatacggcg gcattcgaca gtttatttcc agatgttgac aattacctgg ggcctatggg
     1081 ttttggcctt tccggaaatg gcgagctgat acggttatat gacaatgaag ggacgctgat
     1141 cgataccgtg ctttacgatg atgaggatcc ctggcccaca gaacctgatg gcgacggggc
     1201 gactcttgaa ctgatcaacc cggcctggga caatgcgctt gccgaaagct gggcagcttc
     1261 cgaactttac gggacacccg ggaaagagaa tggtaactat gttaaagtcc ccgatcctgt
     1321 tgcaacacct gatatcagtt gccaggtgat cccgaatcca ttcaggtcaa atgctatttt
     1381 ccggatccgg tcggaagaaa ttctgagcaa cgcatccatt gaggtttttg acttgtatgg
     1441 acgcaaagtg aaagagataa acggcattca atccaattat attcctctgt caggccaggg
     1501 cctgaacgca ggaatataca tctacaggat attggatagc aaagggatgg tgctgttcca
     1561 ggggaaattt gtaatgacgg aatagggtaa aaagatgaga ctccatctca aatcttgaga
     1621 tggagtctca ctgatccatc agtcaaaaag atcacccagc cagcctttct ttcttccttt
     1681 atagtaatcg ccatgatggc ggtaatcatc atgatgcccg cgctgttgtg gcggaggttg
     1741 attgaatcct ttcggctgat cttcgttcat tgaccgctcg atgatcttgt ctagctctcc
     1801 acggtcaagc catacgccac ggcattgcgg acaataatct atttcgatgc cctggcgttc
     1861 ggacatcacc aacggaacat tgcaagtcgg acatttcata gctaatattt tttgctaatt
     1921 taaacattta tccttcctga aagtttaacg gttttcatct attgacttct ttattttgtc
     1981 aagaatgaca ccgttctctt ttttccagtc cttcttcccg gcccagaatc cgaagatgat
     2041 caccgacaca aagaatataa tgtaaatgac aagcggccag ggactgtctt cgaaatattt
     2101 gataaagcta taataaacga tgtaaccacc cccggttcca attacaccta agattggaat
     2161 gataatgaac caatctgctg cagtgataaa agtgtacctc ttttctgctt ttcttaaaaa
     2221 tataatgacc ggggctgaat aatcaattct gcggataagg atgatttttc ggaagaaata
     2281 gaccagaata aaaataaaac cggctgccag caattcataa ccgatggaga gcgggccgtt
     2341 tttagtggca atctgtattg catatactcc gagtaaaaca aaaaaaatga tcatgaatcg
     2401 ggataccaat cttttcccct tctggtcagc cgctttcaac tcttcgatca gtccgccaag
     2461 cccatttgca ttatttacat ttttttccgg cacgttaggg gccgaaaacc tatcattcat
     2521 tttgaattct tccagttcca ttttgttcct ccttccattt gatttttatt ttttctttaa
     2581 gacgatgtat cttcaccctt acattactct ccgaaaggcc tgttacatct gctatttccc
     2641 ttgtgctcaa ttcctcaagg taaagcgata cagcagttcg gtcgacgggg gaaagttctt
     2701 ccataaacct ccggaaaaaa tgcagtttat attcttcctg tggattttct tcgataatag
     2761 gttcgtcggg tctgttctca gctgatctta tgcccgacaa ctcaaataga gtctttcttg
     2821 atttatcctt ccgtatgaag gtcaggcagg tattcaccgt gacacggtag atccaggtgc
     2881 tcaactgtga ctggttccgg aatgtcgaca ggttctgcca gatacggatc agtgcttcct
     2941 ggcaggcatc attgcaatct tctctatcct gaaaaaagta acggcaaatg cgaaaaattc
     3001 tgcccttgtt ttcgtctgca atctgcctga ataacacttc tttttccatc aggatcgatc
     3061 ttttgctgat ttagattcag gtacaagtta attgttacaa aatataccaa tataaatcgc
     3121 acatcataat tccaaatccc aaatcgtcaa atcccaaatc gtcaaatcgt caaatcgtca
     3181 aatcgccaaa tccatcagcg cctccgcgat tctcctgtca tcacaaacat gcaccacctt
     3241 gttctgcccg cccagttttc cctgcgattc caggtatctt ttaaatgtcc ctgccggcag
     3301 gggaacgata tgcagcggtt ccaggatacc gccccggatc aggtcgttgt aatatggatt
     3361 acgccggcgc attgcctggt ccaataaccc cggtagagac gttgcatgca acgtctctac
     3421 cggggttgca tgcaacgttt ccaccggggt tgcatgcaac gtttctaccg gggttgcatg
     3481 caacgtttct accggggttg catgcaacgt ttctaccggg g
//
LOCUS       DAOPNH010000320         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_31928_length_3515_cov_4.829769, whole genome shotgun sequence.
ACCESSION   DAOPNH010000320 DAOPNH010000000
VERSION     DAOPNH010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31928_length_3515_cov_4.829769"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKZ74_13340"
     CDS             <1..1023
                     /locus_tag="PKZ74_13340"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPM93785.1"
                     /translation="CYAFSQDTFSIVAVDTVTGEIGSAGASCIDESQIEGGALIISDV
                     MAGRGAIHTQSYWNATNQQNAHNRMVEGMSPQEIIDWLTTHDAQGNPAVRQYGVVDFD
                     EEGHARAAAFTGNNCMNYKNHIVGPNYAIQGNILLGQQILDSIEARFLNTQGSFAERM
                     MSALQGANVVGADTRCTNNGTSSLSAFIRIARPQDTTGTFYCDLNVPSVPDGMEPIDS
                     LQVIFDEWLETAVGIDENADQDGFVIYPNPAGTWFSVGQHVLSPAKPGEGSHLDNSAP
                     GLTSSKDHRIFIQNMLGERIREIEIQGNLDGIRIDVSECPNGLYFVSIYSDSVLKTSN
                     KLIILR"
     gene            complement(1032..1478)
                     /locus_tag="PKZ74_13345"
     CDS             complement(1032..1478)
                     /locus_tag="PKZ74_13345"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPM93786.1"
                     /translation="MTIIRISKEFNFDMAHALLGYDGLCRNIHGHSYTLVVTIIGTPV
                     ADPASPKTGMLIDFKDLKNIIKTNIVDPYDHALVLNADTPKDLLDTLVRNYEKIVVTP
                     YQPTTENMIADLADRISRLLPGHVKLYSLKLRETPSSFAEWYAEDQ"
     gene            complement(1533..2090)
                     /locus_tag="PKZ74_13350"
     CDS             complement(1533..2090)
                     /locus_tag="PKZ74_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HPM93787.1"
                     /translation="MPYTILLFPLIISFIPLSVAVSTSIYRCVEWKEAFRTAIVFALF
                     QAAMMTIGWAIGYGVKGMLYSMAVPIGAVIVFFIGIRLMFDSRRLTRENRIMAVENRR
                     ILIGFALVSSINTALLGMGLGLLYPDILFLAAPVFGFTFLGVVAGVQAGKRGMVNLGR
                     NLELLGSLGLIIASIIIALQHLRIL"
     gene            complement(2113..2565)
                     /locus_tag="PKZ74_13355"
     CDS             complement(2113..2565)
                     /locus_tag="PKZ74_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPM93788.1"
                     /translation="MNKEERYQRIYNQLDELLETGHGPLSRMATIVAVLHHKMEYFFW
                     TGFYLLEKGELMVGPYQGSLACIRLKKDTGVCWAGINRAETVIVPNVHAFPGHIACDS
                     RSKSEIVVPLKDRSGIIMGVLDVDSKELASFDETDALWLQKITNLVYL"
     gene            2703..3278
                     /locus_tag="PKZ74_13360"
     CDS             2703..3278
                     /locus_tag="PKZ74_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPM93789.1"
                     /translation="MNSIKGTQTEKNLLKAFAGESQARNRYEFYAKEAKKEGLEQIAA
                     IFEETAENERSHAKQFFRFLEGGMVEITAAYPAGIIGNTLENLKAAADGENEEWTHLY
                     PEFARTAEQEGFKEVAAAFKIISKIEKAHEERYRKLYDNLESGKVFEREGKVVWKCRV
                     CGYLHEAKKPPLNCPACHHPQAYFELHTENY"
     gene            complement(3272..>3515)
                     /locus_tag="PKZ74_13365"
     CDS             complement(3272..>3515)
                     /locus_tag="PKZ74_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPM93790.1"
                     /translation="IGTLLADRDPVTRENRYPKLELMRLTIPRRVYTNNHMDYIAACL
                     KNIYDRREEITSGLRIVREAPILRHFTIELDKFGDQ"
BASE COUNT          915 a          857 c          909 g          834 t
ORIGIN      
        1 tgttatgctt tttcccagga cactttctcg atcgtagccg tagatacggt gacgggtgag
       61 atcggcagcg ccggcgcatc ctgtatcgac gagagccaga tcgaaggggg cgccctgatc
      121 atcagcgatg tgatggccgg acgcggagct atccataccc agtcgtactg gaatgccacc
      181 aaccagcaga atgcccacaa ccggatggtg gaagggatga gcccccagga gatcatcgac
      241 tggctcacaa cacatgatgc gcaggggaat ccggccgtca ggcagtatgg tgtcgtcgat
      301 ttcgatgaag agggacatgc acgggctgcc gcattcaccg ggaacaactg catgaattac
      361 aaaaatcata tcgttggacc gaattatgcc atacagggaa atatcctcct gggacagcag
      421 atcctcgaca gtattgaggc acggtttctt aatacgcagg gaagttttgc cgagcgaatg
      481 atgtcggccc tgcagggagc aaacgttgtt ggagccgaca cccggtgcac caacaacggc
      541 acctcatcgc tctctgcatt tatccgtatc gcccgtccgc aggacactac cggcactttt
      601 tactgcgatc tgaatgtccc ttcggtaccc gacgggatgg aaccgatcga ctcgctccag
      661 gttattttcg acgaatggct ggagactgcc gtaggaattg atgagaatgc agaccaggac
      721 ggtttcgtca tctacccgaa tccggccgga acgtggtttt cagttggaca gcatgtcctg
      781 agtccggcga agccgggcga aggatcacac ttggacaatt cggctcccgg gttgacttct
      841 tcaaaggatc acaggatttt tattcaaaac atgctgggtg aaaggatccg ggagattgaa
      901 atacaaggaa atcttgacgg gatcaggata gatgtttccg aatgtccgaa tggcttatac
      961 ttcgtatcta tctattccga ttcggtatta aaaacttcaa ataagctgat tattctcagg
     1021 taattatctg actactgatc ctccgcgtac cattccgcaa aagagcttgg ggtctcccgc
     1081 agtttcaggc tgtatagttt cacatggccg ggcagtaacc ggctgatccg atcggcgagg
     1141 tcggcgatca tattctctgt tgtcggctga taaggcgtta ccacgatctt ttcatagttc
     1201 ctgaccaatg tgtcgagcag gtccttcgga gtatcggcat tcagtacaag ggcgtggtcg
     1261 taggggtcca caatatttgt tttaatgatg ttcttcaggt ctttgaaatc gatcagcatt
     1321 ccggttttgg gagatgccgg atcggccaca ggggtaccga tgatagtgac cacgagcgta
     1381 taggaatgtc cgtgaatatt cctgcacagg ccgtcataac ccaggagtgc atgagccata
     1441 tcgaagttga attctttact gatccggatg attgtcattt ggaaatgtga taatttggaa
     1501 atttgaaaat tagttaattt gaaaatgctg atttataata tgcgaaggtg ttgcagagct
     1561 atgataatac tggcgatgat gaggcccagg cttcccagga gctccaggtt ccggcccagg
     1621 ttgaccatac cccgtttccc ggcttgaacg ccggctacga cgccaaggaa agtaaaaccg
     1681 aagacagggg ccgccaaaaa cagtatatcc gggtagagaa gtcccagccc catcccgagg
     1741 agtgctgtat tgatactgga taccagggcg aatccgatca aaatgcgccg gttctccacc
     1801 gccatgatcc ggttctcccg ggtaagccgc cgggaatcga acataagcct gatgccgata
     1861 aagaatacga ttaccgcgcc gattggcacg gccatgctgt aaagcattcc tttgacgcca
     1921 tacccgatgg cccagccaat agtcatcatc gccgcctgga acagggcaaa gacgattgct
     1981 gttctgaacg cctctttcca ttccacacac cggtaaatgc tggtgcttac cgcaaccgat
     2041 agcggtataa agctgatgat gagcgggaaa agcaagatag tatacggcat ggctgaggct
     2101 atatgttaat gatcagagat ataccaggtt tgtaatcttt tggagccaca gggcatcggt
     2161 ttcatcaaat gaagccagct ctttgctgtc gacatccaga acgcccatga tgataccgct
     2221 acgatccttc agtggcacaa cgatttccga ctttgacctc gaatcgcagg cgatatgacc
     2281 cgggaaagca tgcacattgg gtacgatgac cgtttctgcc cggttgatcc cggcccagca
     2341 aacgccggtg tcttttttca gccggataca ggccaggctg ccctggtaag gccctaccat
     2401 cagttcgccc ttttctaata ggtaaaatcc ggtccagaag aaatattcca ttttatgatg
     2461 aagaaccgcc actatcgttg ccatgcggga taaaggccca tggcccgttt ctaacagctc
     2521 atcgagttga ttgtaaatcc gttggtatct ttcttcttta ttcatttaaa taattaaaat
     2581 aatgaaatca tcttaaatgc cgttgcttct cctaatttct accatgaagg cacgaagaca
     2641 ctaagaggga tcaaaaataa atataattca gtatctttat tcgctaacaa aaaccaatac
     2701 ccatgaacag tataaaaggg acgcagaccg aaaaaaacct gctgaaggca tttgccggtg
     2761 aatcgcaggc acggaaccgt tatgaatttt atgccaaaga ggcaaagaaa gaaggattgg
     2821 agcaaattgc tgcaattttt gaagaaaccg ctgaaaatga gcgctcccat gccaagcaat
     2881 ttttccgttt tctcgagggc ggaatggtgg aaattaccgc tgcttaccct gccggcatca
     2941 tcggaaatac gctcgagaac ctgaaagcgg ccgccgatgg cgagaatgag gagtggaccc
     3001 atctataccc cgaatttgcc cggactgccg agcaggaagg gtttaaggaa gtggccgctg
     3061 ccttcaaaat catctcgaag atagagaaag cgcacgagga gcgttacagg aagctttacg
     3121 acaacctgga atcagggaaa gtgtttgagc gtgaagggaa agtggtctgg aagtgccggg
     3181 tatgcggata cctgcatgaa gccaaaaagc ctccgctgaa ctgcccggcc tgccatcatc
     3241 cgcaagccta cttcgaatta cataccgaaa actactgatc cccgaactta tccagttcta
     3301 ttgtgaaatg cctgagtatg ggggcttctc gcacgatccg gaggcctgag gtgatctctt
     3361 cgcggcggtc gtaaatattt ttcaggcacg cagcgatata atccatgtgg ttgttcgtat
     3421 ataccctccg ggggatggta aggcgcatca gttccagttt cggataccgg ttttcccgtg
     3481 tcaccgggtc gcggtcggcc agcagggtgc caatc
//
LOCUS       DAOPNH010000321         3444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_32841_length_3444_cov_4.690174, whole genome shotgun sequence.
ACCESSION   DAOPNH010000321 DAOPNH010000000
VERSION     DAOPNH010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32841_length_3444_cov_4.690174"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKZ74_13370"
     CDS             complement(<1..199)
                     /locus_tag="PKZ74_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM93791.1"
                     /translation="MDKIRILWVDDEIEILKPHILFLEQKGYEVETSNNGDDGLEMIL
                     NRYYDIVFLDENMPGLTGIETL"
     gene            374..1399
                     /gene="lpxD"
                     /locus_tag="PKZ74_13375"
     CDS             374..1399
                     /gene="lpxD"
                     /locus_tag="PKZ74_13375"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPM93792.1"
                     /translation="MEFSASQIAAVLGGQTEGNPDVKISRLSKIEEGEPGTLSFLANP
                     KYTPYIYTTKASIIIVSRDFIPDQPVSATLVRVDDAYTAFAKLLELYNQIKNEKKGIS
                     SLAFISKSAVIGPDVYIGEFTFIGENVVIENNTKIFPHVFLGDNVKVGSHTTLFAGVI
                     VYSDNSIGSNCTIHGGVVIGADGFGFAPQQDKNYKKVAQIGNVVIEDNVEIGSNTTID
                     RATLGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQTGISGSTKIGKNCMIAGQVGFV
                     GHLIIGDDVKIGAQSGVSGNLKEGSISMGSPAIDIAIHRRALVHFKNLPGIVKRIDEL
                     EKRLNKE"
     gene            1444..2865
                     /locus_tag="PKZ74_13380"
     CDS             1444..2865
                     /locus_tag="PKZ74_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPM93793.1"
                     /translation="MTENQKTIKRPVSLAGVGLHTGQEVNLTFRPAPINYGRRFVRVD
                     LDGRPEIEAVVENVVDTARGTSIASNGAKVQTVEHVLAAVAGSGIDNIIIEVDQAETP
                     IMDGSSRFFVEALDQCGIQEQPALKEYLELRTPVFYEEPENKIEMVAMPYPRFKVTTM
                     INFETKVLGAQYAIMENMEDFRREISMCRTFVFLHELEFLLKNELIRGGDLNNAIVFV
                     NRAVSQDELNRLAKLFNKPSVEVLNEGILNNLELHFENEPARHKLLDVVGDLSLLGKP
                     IKAHIIARRPGHTANAKFAQLIRQQLLKDKKMTQAPLYDPNKPPILDINQIKNILPHR
                     PPFLLIDKIIEMTETRVVGVKNVTMNEQFFEGHFPDEPVMPGVLQVEAMAQAGGVLVL
                     STVPNPQDYTTLFLKIDQVKFRQKVVPGDTLIFDLKLAGPIRRGIAHMEGVAYVGNKV
                     VMEAVMMASIVEKGTNNIANPKK"
     gene            2862..>3444
                     /gene="lpxA"
                     /locus_tag="PKZ74_13385"
     CDS             2862..>3444
                     /gene="lpxA"
                     /locus_tag="PKZ74_13385"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPM93794.1"
                     /translation="MIQPLAYVHPQAKVANNVIIDPFVTIEKNVEIGEGSWIGHHATI
                     MEGARIGKNCRIFPGAVISAIPQDLKFDGEDTTVHIGDNTTIREFVTVNRGTKASYKT
                     VIGNNCLLMAYVHVAHDCIIGNHVILANAATLAGHIEIDDFAIVGGLAAVHQFVKIGA
                     HSMISGGGMARKDIPPYTKAAREPLSYVGVNSIG"
BASE COUNT          968 a          786 c          835 g          855 t
ORIGIN      
        1 ccagagtctc gatcccggtc agtccgggca tattctcatc cagaaatacg atatcataat
       61 aacggttaag gatcatttcc aggccatcat ccccattatt ggaagtttcc acttcatacc
      121 ctttttgttc aagaaaaaga atgtgtggtt tcaatatttc aatctcatca tcaacccaga
      181 gaattcttat cttatccatg cttaaatatt atttagaata aagacttata ttaagaaatg
      241 aaaataatag atatttattg ttcagatgat gaattatccg tgaaaagtgg tttcgacaaa
      301 caggccttca aatttataat tttaatacct ttgtcccacc taaaaacatt gttttcaaat
      361 aaatccgatc cgcatggagt tttcagccag ccagatagcc gctgttttgg gaggacagac
      421 agaaggtaat cctgacgtaa agatcagcag gctatccaag attgaagaag gagaaccggg
      481 aacgttatca tttcttgcca atccaaagta caccccgtac atttatacta cgaaagcatc
      541 cattattatt gtgagccggg attttattcc cgaccagccg gtatcagcca ccctggtccg
      601 tgttgatgac gcctataccg catttgccaa acttttggag ctgtataacc agatcaagaa
      661 tgagaagaaa gggatttcct ccctggcttt tatttccaag tcggctgtta tcggtccgga
      721 tgtttatatc ggtgaattta cctttatagg cgaaaatgtt gtgatcgaaa acaatacgaa
      781 gattttcccc catgttttcc tgggtgataa tgtgaaagtc ggaagccata cgactttgtt
      841 tgccggcgtt attgtatatt ccgacaactc catcggaagc aactgcacca tccatggcgg
      901 tgtggttatc ggggcagacg ggttcgggtt tgcaccgcag caggataaga attacaagaa
      961 agtagctcag atcggcaatg tcgtcattga agacaatgtt gaaattggct ccaacaccac
     1021 gatagaccgg gctacattgg gatcgaccat tatccggaaa ggggccaaac tcgacaatct
     1081 tatccagatt gcgcacaatg tagagattgg cgagaatacg gttattgctg cacaaacagg
     1141 catatccgga tcaacaaaga tcggtaagaa ctgtatgatt gcggggcagg tcggatttgt
     1201 ggggcacctc attatcggcg acgatgtaaa gatcggagct caatcaggcg tttcgggcaa
     1261 cctgaaagaa ggctctatca gcatgggttc cccggccata gatattgcca ttcatcgccg
     1321 cgccctcgtc catttcaaaa atcttcccgg gatcgttaaa agaatcgacg aactcgaaaa
     1381 gcggcttaac aaggaatgat ccggatcgcg aacgggaaga aatgttaatt ttaccggcta
     1441 gttatgacag aaaatcaaaa aacaataaaa agaccggtca gccttgccgg agtcggattg
     1501 catacgggcc aggaggtgaa tctgactttc aggccagccc caattaatta cggaaggaga
     1561 tttgtccggg tagatttgga tggccggcct gagattgaag ccgttgttga aaatgtggtg
     1621 gatacggccc ggggcaccag cattgcgagc aacggggcca aggtgcagac cgtcgaacat
     1681 gtactggctg ccgtagccgg atcgggtatc gacaatatca tcatcgaagt agaccaggct
     1741 gagaccccca ttatggacgg cagttccagg ttttttgtgg aagcgctcga ccagtgcggc
     1801 atccaggaac agcccgcttt aaaggaatat cttgagctca ggacgcctgt tttttatgaa
     1861 gagcctgaga ataagatcga gatggtggct atgccctacc ccaggttcaa agtcactacg
     1921 atgatcaatt ttgagaccaa ggtcctgggc gcacaatacg cgatcatgga aaatatggag
     1981 gatttccgca gggaaatctc catgtgcagg acttttgttt ttttacatga gcttgaattt
     2041 ttgctgaaaa acgagctgat ccgcgggggt gatctcaata acgccattgt atttgtaaac
     2101 agggcggtga gccaggatga gctcaacagg ctggccaaat tgttcaataa accttcggta
     2161 gaggtattga atgaaggtat tttaaacaac cttgaactcc attttgagaa tgaaccggcc
     2221 cggcataaat tactggatgt ggtcggcgat ctgtcgttgc tcggcaaacc gatcaaagcc
     2281 catatcatag cccgcaggcc gggacatacg gccaatgcga aatttgcgca actcatcagg
     2341 caacaactct taaaagataa aaagatgaca caggcaccgt tgtacgatcc aaacaagccg
     2401 ccgattcttg acatcaacca gatcaagaat atattaccgc accggccgcc gtttctgctg
     2461 attgataaaa tcattgaaat gactgagaca cgtgttgtcg gggtgaaaaa tgttactatg
     2521 aatgaacaat tttttgaagg tcattttccg gacgagccgg tcatgccggg tgttctgcag
     2581 gtcgaagcca tggcccaggc cggcggtgtg ctggttttaa gcacggtacc caatccccag
     2641 gactatacga cccttttcct gaaaatcgac caggttaaat tccgtcagaa ggtcgttccg
     2701 ggcgataccc ttattttcga cctgaaactg gccggcccga tcagaagagg cattgcccat
     2761 atggaaggtg ttgcttatgt cggcaataaa gtggtgatgg aagcagttat gatggcttcc
     2821 atcgtagaaa aaggaactaa taatatcgca aacccaaaga aatgatccag ccgttagctt
     2881 acgttcatcc gcaggcaaaa gtcgccaata atgtcattat cgaccctttc gtcactattg
     2941 aaaaaaatgt ggagatcggg gaaggttcct ggatcgggca ccatgcaacc attatggaag
     3001 gggcccggat cggtaaaaat tgccggattt ttcccggagc cgttatttca gccattccac
     3061 aggatctgaa attcgacggg gaagatacca cggtccatat tggtgacaat acgaccatcc
     3121 gcgaatttgt taccgttaac cgggggacaa aagccagtta taaaacggtg atcggcaaca
     3181 actgcctgct gatggcctat gtgcatgtcg ctcacgactg cattatcgga aaccatgtga
     3241 tcctggccaa cgctgccacg ctcgccggtc acattgagat agacgacttt gcgattgtcg
     3301 gcggcctggc ggctgttcac cagtttgtca agatcggcgc ccactccatg atctcgggcg
     3361 gcggtatggc ccgtaaagac atacctccat atacgaaagc agctcgtgaa cccctctcct
     3421 atgtcggggt taactccatc ggac
//
LOCUS       DAOPNH010000322         3435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_32959_length_3435_cov_4.118639, whole genome shotgun sequence.
ACCESSION   DAOPNH010000322 DAOPNH010000000
VERSION     DAOPNH010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32959_length_3435_cov_4.118639"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2852
                     /locus_tag="PKZ74_13390"
     CDS             <1..2852
                     /locus_tag="PKZ74_13390"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM93795.1"
                     /translation="RMIYSPAKHPVPVSQPAQPGELIRNTLIQDFESYADFSLVFNPW
                     TTNDVDGSTTYGIQDVEFPHAGEPMSYIVFNPASTTPSMSGDAALQPHGGSRFAACFA
                     STTPANNDWIISPQIALGSSGVLKFWVKSYTSQYGLERYKVGISTTTNNPSSFTIISP
                     GAYLEAPAGAWEQKTFDLSAYSGQNIYVGIQCVSNDAFIFMLDDLEVTSQTVTGGTLT
                     GKVTDAINGNPIQNALVSIAGLTDLTDANGNYAITGIPAGALNAEFNATPTSGTAPLA
                     VQFTDLSTEGTQSVTCSATGYTTYTNNQVVIPAGGSLELQISLSPTLAAGQYRVVLTW
                     GEQPLDIDSHLKTPVIEGTAYHIYYQYFGSVSAPPYAQLDIDDTESFGPETVTIYQLK
                     PGEYHYYVHNYSQTPAITTSNAVVQVYNENGLMQTFNVPTAGEGLYWDVFKLSGSGVI
                     TPVNQIVTAEPGSAPFLTPEQMVKKPAIGPKNIVSWNWTFGDGATSTQQNPNHTYAAN
                     GTYTVSLTVSDGTNSNTETKTGYITVTGGSGGTATLTGKVTDAVNGNPVPNALVSIAG
                     LTDLTDANGNYSITGIPAGALKANFTANPTAGTAPLVVQFTDLSAENAHIVTCSATGY
                     TTYSNSQVVIPDGGSLELQISLSPILAAGQYRVVLTWGEQPLDIDSHLKTPVIEGAAY
                     HISYQNFGSAATPPYALLDIDDTESFGPETVTIYQLKPGEYRYYVHNYSTSPEITVSN
                     AVVQIYNESGLLYTFNIPTSGSGLYWDVFKLNGSTGAVTSINQIVANEPGGKDFYIPG
                     KFEKKQVNTKRNVVSWSWNFGDGGVSTEQNPSHKYLNNGSYTVSLTVSDGTANNTETK
                     NAYIVVGPAGTEEPGWEKEVSIFPNPANDKLNINSGVPVHSASLVDMNGNPVIMLTEC
                     GKSFSIDAHNLPNGVYILRIITDRGMMVRKVNIQR"
     gene            3193..>3435
                     /locus_tag="PKZ74_13395"
     CDS             3193..>3435
                     /locus_tag="PKZ74_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93796.1"
                     /translation="MKKILILWIIPDLLLVTLVLPLSGQWAINGSHIYNTNSGNAGIG
                     TSTPASLLYVARNTTEPTVTIRNSGGSGGATFSMVDV"
BASE COUNT          913 a          851 c          788 g          883 t
ORIGIN      
        1 agcgaatgat ttattcgccg gctaagcatc cggtaccggt atcacaacct gcccaacccg
       61 gtgaattaat cagaaatact ctcatccagg attttgaatc gtatgccgat ttcagcctgg
      121 ttttcaaccc gtggacaacc aatgatgtgg atggatctac gacctacggg attcaggatg
      181 ttgaatttcc gcacgccggg gaaccgatgt cctatatcgt tttcaatccg gcttcgacga
      241 caccttcaat gagtggtgac gccgctcttc agcctcatgg aggcagccgg tttgccgctt
      301 gtttcgcttc gacgacaccg gccaataatg actggatcat ctctccgcag attgcgcttg
      361 gttccagcgg ggttctgaaa ttctgggtca agtcgtatac cagccagtat ggacttgaaa
      421 gatacaaggt tgggatctct actaccacta acaatccctc cagttttacc atcataagcc
      481 ctggagcata cctggaagct cctgccggcg cctgggaaca gaaaaccttt gatttatcgg
      541 cttacagcgg gcaaaatatt tatgtcggca tccagtgcgt ttccaatgat gcctttattt
      601 ttatgctgga cgacctggaa gtgacttccc aaacggttac gggaggaacc ctcacaggta
      661 aggttaccga tgccataaac ggtaatccga ttcaaaatgc cctggtgagc attgccgggc
      721 tcaccgatct cacggatgcc aacggaaact acgccatcac gggaatccct gccggtgcgc
      781 tcaatgctga attcaacgct acgcccactt ccggtaccgc gccactggct gttcagttta
      841 ccgatctgtc gaccgaagga acccagtctg ttacctgttc agcaacaggc tatacaactt
      901 atacaaataa ccaggtggtg attcctgcag gaggatctct ggaattgcag atttcccttt
      961 caccaacgct ggcagccggt cagtaccgtg tggttttaac ctggggagag cagccgctgg
     1021 atatcgactc gcatctgaaa actcctgtca ttgaaggaac ggcttatcat atttattatc
     1081 aatatttcgg atcggtcagt gctccgcctt atgcccagct tgatatagat gacacggaaa
     1141 gtttcggacc ggaaaccgtt accatatacc agcttaaacc cggggaatac cattattacg
     1201 tccataatta ctcgcaaact cctgcgatca caacatccaa tgcagttgtc caggtttaca
     1261 atgaaaatgg cctgatgcag acgttcaatg tccccacagc cggagaagga ttgtactggg
     1321 acgtcttcaa gttaagcggc tccggggtga tcacaccggt gaaccagatt gttactgcag
     1381 agcccggttc ggctcctttc ctgacacctg agcaaatggt gaagaagcct gcgatcggac
     1441 caaagaacat tgtttcctgg aactggactt tcggagacgg cgcaacgagc acccagcaaa
     1501 atccgaatca tacttacgct gccaacggaa cctatacggt ttcactgacc gtttccgacg
     1561 gaaccaacag taataccgaa accaagaccg gttatatcac ggtgacggga ggcagcggtg
     1621 gcacagcgac actgaccgga aaggtgaccg acgcggtgaa cggcaatccg gtaccgaacg
     1681 ccctggtttc tattgccggc ctgaccgatc ttactgatgc caatggcaat tattcgatca
     1741 ccgggattcc tgctggcgct ctcaaagcca attttaccgc taatcccaca gccggtactg
     1801 cgccgctggt tgtacagttt accgatctgt cggcagaaaa tgcccatatt gtaacctgtt
     1861 cagccacagg atataccaca tattccaata gccaggtggt tattccggac gggggatcgc
     1921 ttgaactcca gatttcattg tcgccgatac tggctgccgg acaataccgg gttgtgctga
     1981 cctggggcga acagcctctg gatatcgatt cacatctcaa aacgcctgtt atagaagggg
     2041 ctgcctatca catctcctat cagaattttg gatctgccgc cactccgcca tatgcactgc
     2101 ttgatataga cgatacggaa agcttcgggc ctgaaaccgt tacaatctac caattgaagc
     2161 caggcgaata ccggtattat gtccataatt attctacatc gccggagatc accgtttcaa
     2221 atgccgttgt ccagatctat aacgaatcag gattacttta tacattcaat attccgactt
     2281 ccggttcggg tttatattgg gatgttttca aactgaacgg ttcaacagga gcagtaacgt
     2341 ctataaacca gattgtagcc aacgagcccg gtggcaaaga tttttacatc cccggtaagt
     2401 ttgaaaagaa acaggtaaac acaaagagaa atgttgtttc ctggtcatgg aatttcggtg
     2461 acggcggcgt cagcaccgag caaaatccat cgcataaata tctgaacaat ggaagctaca
     2521 ccgtttctct gaccgtttcc gacggtactg caaacaacac tgaaaccaaa aatgcctata
     2581 tcgtggtggg gcctgcaggg accgaagaac ctggctggga aaaggaagtt tccatattcc
     2641 cgaatcccgc caatgataaa ttgaatatca attcaggagt tccggttcat tcagccagcc
     2701 tggtcgacat gaacggaaac ccggtgatca tgctcactga atgcgggaaa tcattttcga
     2761 tcgacgcaca caacctgccc aatggggtat acatcttaag gatcattacc gacagaggga
     2821 tgatggtcag aaaagtaaat attcagagat agttccttcc catttcgcta ttattgaaga
     2881 ggaggtctca aaagaatttt accacaaagg aacacaaagg aattcaccaa ggcgcactaa
     2941 ggtttattaa ttggtattta attacttagt gttccttcgt ggtgtacttt gtgcgccttc
     3001 gtggttaata aaaagccttt tgagacggcc tcttttttta ttgactttat cccaatattt
     3061 gattatatta gcaccggtta tttgttaaca cgataaacca ttcctttcaa atggcttata
     3121 gttaggaaag acctttctag cggtctgttc tgaagccatg ataataatca tcttcataaa
     3181 tcctataccc atatgaaaaa gatattaatc ctttggatta ttccggattt gctattggta
     3241 actttggttt taccgttatc cggacagtgg gccattaatg gcagtcatat ttacaatacg
     3301 aattcgggta atgcaggtat cggtaccagt actcctgcca gtctactgta tgttgcacgg
     3361 aatactacgg agccaaccgt tacgatacgg aactcaggcg gttcaggtgg tgcgacattt
     3421 tcaatggtgg atgtg
//
LOCUS       DAOPNH010000323         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_33011_length_3430_cov_8.170074, whole genome shotgun sequence.
ACCESSION   DAOPNH010000323 DAOPNH010000000
VERSION     DAOPNH010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33011_length_3430_cov_8.170074"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKZ74_13400"
     CDS             <1..895
                     /locus_tag="PKZ74_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93797.1"
                     /translation="VLKCRPNYTIPAGYYITNIVFTIKWPETSPVTQLQNIVSTVNSY
                     YQITPQQFSVNGGYRYQVYSMVGAKNVTWSANQEYPVLEIRVNYPGGCTNLEIANDSY
                     TNFVLNGAYYISITGSNKTGIRYQPSVSLISQGGVVTPDESLCLGSSTSVMVLSGYSG
                     NILMWQKKHDSFGWINISGTAGMWEYFTTPDSSGTYRYRAVVKRGSCDTAYSTANVVG
                     VAANSVWTGSADTLWHNPSNWNACGVPAASRDVEIPAVAGGLYPAITAPGVCKSLLIR
                     SGATLKLKGSGSLDIDGTLVK"
     gene            935..3283
                     /locus_tag="PKZ74_13405"
     CDS             935..3283
                     /locus_tag="PKZ74_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93798.1"
                     /translation="MNSCKVISFLAVTCLITLPSFSQQEEIDAGIFSGMTADTLILKA
                     KPSFTLSGQSLTNAQITVKWSQNSPVTSLQWNYSPIGLFPQGQTEISEGYRYQVYGSF
                     GGSGINWVAGQEYVILKLVTNSPSGSCTEFLIAQDDWTQNNNGDYYMEVLGEDKTGLL
                     YEPSVNFGSVGGNIVSDSALYLGNSTGIMELTGYSGDIVYWERSINDGAWDILPGTAG
                     QAQFEDVPTIEGTWSYRVKVEKPGCQPVYSAPGDITVELLAIWTGAVNNSWDDPGNWN
                     VAGVPDENLDGLVPQTETGMYPVINGYENGKNLLIGPGASLILSKEGALTLSGYLKND
                     GLFSMESDTGASASLLDNGISGTGVFVSSVIYTPGLHHQISSPVIHSAKNAFGNTDLF
                     EWNEPQNEYIPVPDDDDTLEVLKGYLAINNDQVMNPAVFEGTGLFSGIYSADFTNNGA
                     GNGHAGGFNLAGNPYPSAMNWNDAENVTLSQVDPALYVLNGEAGNFGTFIRGDHASST
                     LGIDSIIGTHQGFFVHVNHASDSGSLTVNNGARFHNPQPLRAATAARSDDPVLKLKVS
                     GPEGSFRDEIIVRFHSEASEYFDIQYDAYDLEGFQNAPQVFTRCADTLRLAVNTYPSL
                     DANRSVVMGFQSGEAGEFTLAVQELLNFGTDTLVFVEDLILDTTFRVTESFQYVFEGT
                     PSDPVDRFRLHFIVDPVFEDEGSGLPEISITLAGQIMRVNFGGESQCGYFELFDLTGK
                     RLMSQRVTGPVAMIHFPLESGMYIIRINIQKAVLVKKIIACF"
BASE COUNT          844 a          827 c          903 g          856 t
ORIGIN      
        1 ggtgctgaaa tgccggccga actacactat cccggcggga tattacatca ccaacatcgt
       61 gtttaccata aaatggccgg aaacgagccc cgttacccag ctccagaata ttgtttccac
      121 cgttaactcc tactaccaga tcacgcctca gcaattttcc gtcaatggag gataccggta
      181 ccaggtttac tcgatggtcg gggcgaaaaa tgttacctgg agcgccaacc aggaataccc
      241 ggtacttgag atccgggtga attatccggg agggtgcacc aaccttgaga ttgccaatga
      301 ttcctatacc aatttcgttt tgaacggagc ttattatatt tcgataaccg gttcgaataa
      361 aacgggaatc cggtatcagc catcggtcag cctgatctcg caaggaggcg tggtaacgcc
      421 ggatgaatcc ctatgcctcg gcagcagtac ttccgtgatg gtcttatcgg gctattcggg
      481 aaatatcctg atgtggcaga agaaacacga ttcctttggc tggatcaata tttcaggaac
      541 ggcggggatg tgggaatatt tcaccacgcc cgattcatcg ggaacatacc gctacagggc
      601 cgtggtaaaa agggggagct gcgatacggc ctattcgacg gccaatgtag ttggcgtggc
      661 ggcaaactcc gtctggacgg gcagcgccga cacattgtgg cataatcctt cgaactggaa
      721 tgcctgcgga gtgcccgcag ccagccggga tgtcgaaatt ccggccgtgg ccggtggatt
      781 atatccggcc attacggcgc ccggcgtttg caaatccttg ctgatccgat cgggagccac
      841 attgaaatta aaaggttcag ggagcctgga catcgacggt acactggtaa aataattcgc
      901 atagcaatct tttccggcca aacagacact taatatgaat tcctgtaaag ttatatcgtt
      961 cctggcagtc acctgcctga tcacactgcc gtcgttttcg cagcaggaag agatcgatgc
     1021 gggcattttt tccggcatga ccgccgatac ccttatttta aaggctaaac cctcattcac
     1081 cctttccggt caatccctga ccaatgcaca gataacggtc aaatggagcc agaacagccc
     1141 ggtgacctcc ctgcaatgga attacagtcc gatcggattg ttcccgcagg gccagacgga
     1201 aatttcggaa ggataccggt accaggttta cggttctttc ggcggttcgg ggatcaactg
     1261 ggtggccgga caggaatatg ttatcctgaa actggtcacc aattcaccgt cgggctcctg
     1321 taccgagttc ctgattgcgc aggatgactg gacacaaaac aacaatggcg attattacat
     1381 ggaagttctg ggagaggata agacgggcct tctctatgag ccttcggtca attttggatc
     1441 ggtcggcgga aatattgtga gcgattcggc gctctatctc ggcaacagca cggggatcat
     1501 ggagctgacc ggttacagcg gggatatcgt ctactgggaa agaagtatta acgacggcgc
     1561 ctgggatatc cttcccggca ctgcaggaca ggctcagttc gaagatgtac ccacgatcga
     1621 aggaacctgg tcgtaccggg taaaggtgga gaagccgggc tgccagccgg tttactcagc
     1681 accgggcgat atcaccgtgg aattgctcgc tatttggacc ggtgctgtca acaactcctg
     1741 ggatgacccg gggaactgga acgtcgcggg agtgccggat gaaaacctcg atggcctggt
     1801 cccgcagaca gagaccggga tgtatcctgt gatcaatggt tatgaaaatg gcaaaaacct
     1861 gctgatcggt cccggcgcat ccctgatcct ctcaaaagaa ggagctttga ccttgtcggg
     1921 atatcttaaa aatgacggcc tgttctcaat ggagtccgac accggggcaa gcgcttcatt
     1981 actggataac ggcatctccg gtacaggggt gtttgtcagt tccgtaattt atacccctgg
     2041 tttacatcat caaatttcat ccccggtaat ccattctgca aaaaatgcat tcgggaatac
     2101 ggacctgttt gaatggaacg agccgcagaa tgaatatatc ccggtgccgg atgatgacga
     2161 tacacttgaa gtattaaaag ggtaccttgc cataaataat gatcaggtaa tgaatccggc
     2221 ggtttttgaa gggaccggcc tgttttccgg catttactct gcagatttca ccaataacgg
     2281 cgccggaaac ggacacgccg gcggttttaa cctcgccgga aatccgtatc cttcggccat
     2341 gaactggaat gatgcggaga atgtgactct gtcgcaggtt gatccggccc tgtacgtttt
     2401 gaatggcgag gccggcaatt tcggtacgtt tattcgcggc gaccatgcca gttccacatt
     2461 gggtatcgac agcattatcg gcacccacca gggttttttt gtgcatgtaa atcatgcttc
     2521 cgattccgga tctctgacgg tgaacaatgg ggcaagattt cacaaccctc agcctttgcg
     2581 ggcggcaacg gcagcacgct ctgatgatcc tgtcctgaaa ttaaaagtat ccgggcctga
     2641 aggttcattc agagatgaaa tcattgtaag atttcactcg gaagcatcgg aatactttga
     2701 cattcaatat gatgcctatg atctggaagg gttccaaaat gccccgcagg tattcacccg
     2761 gtgtgccgat accctgagac tggctgtcaa tacttatcct tcgctggatg ccaaccggtc
     2821 tgttgtaatg ggttttcaat ccggtgaagc cggagagttt accctcgcgg tacaggaact
     2881 gcttaatttt ggcacggata cgctggtatt tgtggaagat ctgatccttg acacgacatt
     2941 ccgggttacc gaatcattcc aatatgtttt tgagggaaca ccgtccgacc cggtcgaccg
     3001 gttcaggctt cactttattg tggatcctgt ttttgaagat gaaggaagtg gtcttcctga
     3061 gatttccata acattagcag ggcagataat gcgggttaat ttcggtggag agtcgcagtg
     3121 tggttatttt gaattgtttg acctgaccgg aaaacgactg atgagccaga gggtaacagg
     3181 gccggttgct atgatccatt tcccgctgga aagtggcatg tatataatcc gcattaatat
     3241 tcaaaaagct gttcttgtca aaaaaatcat agcttgcttt tgaaatcctg accggtacgg
     3301 atgatttcat tctgggtaaa atgtatttat ctttacagaa aaaaacatgc ttaccctcat
     3361 attcaggttc cgtttcctga tccttctgcc gttccttggc gggatggtgg ttgtgaacgg
     3421 gcagaacgca
//
LOCUS       DAOPNH010000324         3416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_33200_length_3416_cov_5.710800, whole genome shotgun sequence.
ACCESSION   DAOPNH010000324 DAOPNH010000000
VERSION     DAOPNH010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33200_length_3416_cov_5.710800"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKZ74_13410"
     CDS             <1..897
                     /locus_tag="PKZ74_13410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM93799.1"
                     /translation="VITVSFTFVATVEVIALLKLTPILVDVEPGTFNMDIDALRKAIT
                     PKTRAIVPVHLFGQCANMEEIMDIAREHNLYVIEDACQAIGSDYTFSDGTIKKAGTIG
                     HLGTTSFFPSKNLGCYGDGGAIFTDDDALATRLRFIVNHGMHVRYYHEEIGVNSRLDS
                     IQAAILDIKLKHLDEYAAARQKAAAAYNKAFGGNPHLQVPVKSPQSTHVYHQYTLVTK
                     GIARDMLLDFLLAKNIPCAIYYPVPMHMQKAYLDPRYKEGDLPVTEHLSRTVISLPMH
                     TELDEEQIEYITGTILEFIEKS"
     gene            1060..1641
                     /locus_tag="PKZ74_13415"
     CDS             1060..1641
                     /locus_tag="PKZ74_13415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM93800.1"
                     /translation="MNNRTDYFAHETAVIDDGCRIGAGSKIWHFSHIMAGCEIGEKCN
                     IGQNVVVSPGVKLGHNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIKNPRSAIIRKDQ
                     YATTLVRHGASIGANATIVCGITIGEFAFIGAGAVVVKDVPPYALVLGNPARHIGWMS
                     EFGHRLEFDENNIAVCPESGEKYRLEPGKVSKI"
     gene            1860..2807
                     /locus_tag="PKZ74_13420"
     CDS             1860..2807
                     /locus_tag="PKZ74_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM93801.1"
                     /translation="MTQNFALIGAAGFIAPRHMKAIKDTGNNLVAAVDPFDCVGILDS
                     YFPNTNFFVETERFDRHLDKLRRKGENKVDYVSICSPNYLHDAHIRLALRNEAHAICE
                     KPLVLNPWNADALHEIEIETGRKINVILQLRYHPTIVELKKTIENGPKDKIYDVDLTY
                     ITSRGKWYFISWKGDMEKSGGVATNIGIHFFDMLSQIFGNTTENTVHYSDKFTAAGFL
                     QLERARVRWFLSVDYNTLPDDVRAKGQRTYRSIRIEGKEMEFSDGFTELHTESYRQIL
                     AGQGFGVGDARNSIETVYQIRNAAPAGKKGDYHPFLNRL"
     gene            2898..3377
                     /locus_tag="PKZ74_13425"
     CDS             2898..3377
                     /locus_tag="PKZ74_13425"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93802.1"
                     /translation="MNLYEQLLDKQAKLSVIGLGYVGLPIALEFARKIKVIGFDLNAA
                     RVEMMKNRIDPSEELPSEAFDGCDIEYTADPARLRDAQFHIVAVPTPIDKHNLPDMTP
                     LLKATLTVGKALKKGDYVVYESTVYPGATEEDCIPILELESGLKHIVDFKVDEMLQS"
BASE COUNT          957 a          848 c          835 g          776 t
ORIGIN      
        1 gtcatcactg tctccttcac cttcgtcgcc accgttgagg tgattgcact gctgaaactg
       61 acgcctatcc tggtggatgt cgaacccggc actttcaata tggatatcga tgcgctgcgc
      121 aaagccatca cgccgaaaac cagagcgatc gtgcccgtgc atctctttgg ccagtgcgcc
      181 aatatggaag agatcatgga cattgccagg gagcacaatc tttatgtgat cgaagacgcc
      241 tgccaggcga tcggttcgga ttacaccttc agcgacggga ccatcaaaaa agcgggtact
      301 atcgggcatt taggtacgac gtcgttcttc ccttcgaaaa acctgggctg ctacggcgac
      361 ggcggtgcga tcttcacgga tgacgacgcg ctggctacgc gtctgcgttt tatcgtcaac
      421 cacgggatgc atgtccgtta ctaccacgaa gaaatcggtg tcaactcccg cctcgacagc
      481 atccaggccg ctatcctcga cattaagctg aagcacctcg acgaatatgc cgccgcccgt
      541 cagaaagccg ccgccgccta caataaagcc ttcgggggca atccgcacct tcaggtgccg
      601 gtgaagtcgc cgcaatccac acacgtttac caccagtaca cgctggtcac aaaaggcatc
      661 gcccgggata tgctgctgga tttcctgctg gcgaagaata ttccctgtgc gatctattat
      721 cccgtgccga tgcatatgca gaaggcttac ctggatccac gctataaaga aggcgatctg
      781 cctgttaccg agcatctcag ccgcacagtg atctcgctgc cgatgcatac ggagttggat
      841 gaagaacaga ttgaatatat tacagggaca atacttgagt ttattgaaaa gtcataagct
      901 cgctgcgctc gcgtcgttgg tcattaggaa tgaaaaaagc agaataggac aacaaaacag
      961 caataaaaca gtaaaacaat ctgacagttc aacaatttaa cagtttaaca atttaacgga
     1021 tgaacaattg aacagtttaa caatttaaca gttgaacaat tgaacaatag aacagactac
     1081 ttcgcacatg aaacggccgt tattgatgac ggctgccgga tcggcgccgg atcgaagatc
     1141 tggcacttct cgcatatcat ggccggttgc gagatcggag aaaaatgcaa tatcggccag
     1201 aatgtggtcg tctcaccggg tgttaaactc ggacacaatg tgaaggtgca gaataacgtc
     1261 tcgatttata ccggcgtgat ttgcgaagac gatgttttcc tcggtccctc catggtattc
     1321 accaacatca aaaacccgcg cagtgcgatt atacggaaag atcagtacgc aaccaccctg
     1381 gtcaggcacg gcgcctcgat cggggccaat gctacaatcg tatgcggcat cacgatcggt
     1441 gagtttgcct ttatcggggc aggggcggtt gtggtgaaag atgttccgcc ctatgcgctg
     1501 gtgctgggaa accctgcccg tcatatcggc tggatgagcg aattcggcca caggctggaa
     1561 tttgatgaga ataatattgc tgtatgcccg gagagcgggg agaaatacag gcttgagcca
     1621 ggaaaagtca gcaagattta gttcaaagtt taaagttcaa aattcaaagt ttaaaaaaca
     1681 ctaataatag attaacaatg acaattaatg acgcgagcgc agtgagccaa tgacgcaaac
     1741 gtcagtaagc ctttgacgcg agcgcagtga gctaatgacg cgaacgccag taagcctttg
     1801 acgcgatcga agcgagctaa tgactaatga ttaatgacaa atataatcgc caactatcca
     1861 tgacccagaa ttttgcactc atcggagcag cgggtttcat cgccccacga catatgaaag
     1921 ccattaaaga taccgggaac aacctggtgg cggcggtaga tccgttcgat tgtgtcggga
     1981 ttctggattc ttattttccg aataccaatt ttttcgtgga aacggaacga ttcgaccggc
     2041 atctcgacaa actgcgccgc aaaggggaga acaaagtgga ctatgtatcg atctgctcgc
     2101 cgaattacct gcacgacgcc cacatcaggc ttgcactgag aaacgaagcg catgctatct
     2161 gcgaaaagcc gctggtgctc aatccctgga atgccgatgc ccttcatgaa attgaaattg
     2221 aaacaggccg gaaaataaat gtcatccttc aattacgata tcatccaacg atagttgaac
     2281 taaaaaaaac aattgagaac gggccgaaag ataagatcta tgacgtcgat ctgacatata
     2341 ttaccagccg tggtaaatgg tattttatct cctggaaagg ggatatggag aaatcgggcg
     2401 gagtggccac caatatcggc atccactttt ttgatatgct ttcgcagata ttcggcaaca
     2461 ccacagaaaa caccgttcat tactcggata aattcacagc cgctggtttc ctgcaactgg
     2521 aaagggcccg tgtccgctgg ttcctcagcg tggattacaa tacccttcct gatgatgtaa
     2581 gggccaaagg tcagcggaca taccgctcca tccggatcga agggaaagag atggagttca
     2641 gcgacggttt taccgaactg cataccgaga gctaccggca aatcctggcg ggtcagggct
     2701 ttggcgtcgg agatgcccgg aacagcatcg aaacggtata tcaaatccgt aatgctgcac
     2761 ctgcaggtaa gaaaggcgac tatcacccat ttctgaacag gctttaacgt tcgtgccctt
     2821 tgtgaaaccc tcggtgaacc tctgtaagaa acaaataatc cggaaataag atcattaatt
     2881 gaaacaaaca cttacctatg aatctatacg aacaactgct tgacaaacaa gccaaactgt
     2941 cggttatcgg cctgggctat gtcggactgc ccattgccct cgaatttgcc aggaaaatca
     3001 aggtcatcgg ctttgatctg aacgccgcgc gtgttgaaat gatgaagaac cgcatcgatc
     3061 ccagtgaaga gcttccatca gaagcttttg atggttgcga tatcgaatat accgctgatc
     3121 cggcccggtt gcgcgacgct cagtttcaca ttgttgctgt ccctaccccg atcgacaagc
     3181 acaatctccc cgatatgacg ccgctgctca aagctacgct taccgtgggc aaggcattga
     3241 aaaaagggga ctacgtcgtc tatgaatcga ccgtttaccc gggagctact gaagaagact
     3301 gcattcctat cctggaactg gaatcaggat tgaagcatat cgtcgacttt aaagtcgacg
     3361 aaatgcttca atcctgattc cagttccagg ataggaatgc agtcttcttc agtagc
//
LOCUS       DAOPNH010000325         3386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_33586_length_3386_cov_4.618433, whole genome shotgun sequence.
ACCESSION   DAOPNH010000325 DAOPNH010000000
VERSION     DAOPNH010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33586_length_3386_cov_4.618433"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1251)
                     /locus_tag="PKZ74_13430"
     CDS             complement(109..1251)
                     /locus_tag="PKZ74_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93803.1"
                     /translation="MKNLILTVTAMMLVAVVFGQTNKRTSAFNYNNYGKLDLAKEAID
                     EAAQHEKTIMDAKTWFYRGNIYYNIGVSLDSNYRKLDPDPFGVSLKSYLKAKELDVKG
                     EFKENIDQNIVVISQGYFNMGVFYYNEQNYPLAAKNFEQAYEVSKIANQLDTTALFNA
                     AISARDAHDRATAKKYYLEIIKMNVSNPVVYDSLANIYKAEGDTAIALQTIQQGRQVF
                     KEDLNLVIAETNIYLQTNQQDKALANLETAAKLDPTNPTVFWASGTIYDQLGEQDKAI
                     KAYETAIQLKPDYFVAYFNLGILYFNQATEVMAKANDLPYDTPQDVYDAEKAKAKEIY
                     LKSVPYLEKSFELEPGDMDAANSLRAVYGVLEMADKLKALNESINK"
     gene            complement(1330..>3386)
                     /gene="gyrA"
                     /locus_tag="PKZ74_13435"
     CDS             complement(1330..>3386)
                     /gene="gyrA"
                     /locus_tag="PKZ74_13435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPM93804.1"
                     /translation="EPKVLPAKIPNLLVNGASGIAVGMATNMPPHNLAQVIDGTIALI
                     DNRDITIEELMQYIKAPDFPTGGIIYGYEGVREAFETGRGRIVVRGEAKIEPTPGGKE
                     KIIVTSIPYMVNKAEMIRRTAEIINDKKIEGIVDIRDESDRNGLRIVYELKRDAVANV
                     ILNKLYQMTALQTAFNVNNVALVHGKPKTLNLKELIHYFVEHRHEVVIRRTRYDLDQA
                     EKRAHILEGLLIALDHLDEVIALIRASRTPDEARDGLMREFQLTEIQAKAILEMRLQR
                     LTGLERDKIKLEYEELQKQIAYLRQVLADESLRMKIIKDELTEIKDKYGDEPRTTIEY
                     TAEDFRMEDVIPEEEVVITISHLGYIKRTPLAEYRRQNRGGRGARGSDLRGEDFLEHL
                     FVANTHNYMLLFTEKGKCFWLRTYEIPEGAKNSKGRAIQNLINIETDDKVRAFIKIKD
                     LTDENYLSSNYIILCTQRGIIKKTTLEAFSRPRQAGIIAITVNEGDQLLAAKLTNGTN
                     EVMLALRSGRAIRFNESNVRPMGRNAAGVKGITPGNSNDEVIGMICVETENFDVLVVS
                     ENGYGKRSSLADYRITNRGGKGVKTINITQKTGSLIAIKEVSDSDDLIIINKSGILIR
                     LAVKELRVMGRATQGVRLINLRDEDAIASVAKVEVDESETDDADYDIDPGSEPENGET
                     TE"
BASE COUNT          764 a          857 c          712 g         1053 t
ORIGIN      
        1 cagggataat ttctggcgac atcttggcgt agagtaattt gtcccataat taatattatg
       61 ttaaatgaca attcacaaat taagggggaa cttactcccc cttatctgtt atttattgat
      121 cgattcgttc agcgctttca gtttatcagc catctccaat acgccataaa cggcccgcaa
      181 tgaattagcg gcatccatat cccccggttc aagttcaaat gacttttcca ggtaaggaac
      241 agatttcaga tagatctcct ttgccttagc tttctcagca tcataaacat cctgtggcgt
      301 atcataaggc aaatcattgg cttttgccat cacttccgta gcctggttga aataaagaat
      361 gcccaggtta aaataggcta cgaaatagtc gggttttagc tggatagccg tttcataggc
      421 ctttatggcc ttgtcctgct ctcccagttg gtcgtatatg gtaccactgg cccagaacac
      481 cgtcggattt gtcggatcga gcttggctgc cgtttcaagg ttggccagcg ctttatcctg
      541 ctgatttgtc tgaagataaa tattggtctc ggctataaca agattcaggt cctctttaaa
      601 tacctgacgc ccctgttgaa tggtctgcag agctatagcc gtgtcgccct ccgctttata
      661 aatattggct agactatcat aaaccaccgg attcgaaaca ttcattttga tgatctccag
      721 gtaatatttt tttgccgtgg ccctgtcgtg agcatctctc gccgaaatgg cagcattaaa
      781 taaggcagtg gtatccagtt ggttagcaat cttacttacc tcgtaggcct gctcaaagtt
      841 ttttgctgcc aacggataat tctgttcatt atagtaaaat acacccatgt tgaaatatcc
      901 ctggctgatc accacgatat tctgatcgat attttccttg aactcgcctt ttacatccag
      961 ttctttagct ttcagataag atttgagaga tacaccgaaa ggatccgggt ccagtttccg
     1021 gtaattggaa tcgagactca caccgatatt ataatagata ttccccctgt aaaaccatgt
     1081 ctttgcatcc atgatcgttt tttcatgctg cgctgcttca tcaatcgcct ctttggcaag
     1141 atcgagcttt ccgtaattat tgtaattgaa ggcgcttgtt cttttgttgg tctggccgaa
     1201 tactacagca actaacatca ttgcggtcac tgtcaggatt aagtttttca tacgtaatgc
     1261 tattttatta gttgttaaat cattaacaaa attatcaata ttattaaatc aagcaattat
     1321 tttgccaatt tactcggttg tttcgccgtt ttccggttca ctgccaggat cgatatcata
     1381 atcggcatcg tctgtttccg actcgtcaac ctcaactttt gccacagaag caatggcgtc
     1441 ttcgtcacgg agattgatca gccttactcc ctgggttgcc ctgcccatta cccgtaactc
     1501 ttttacagct aatcggataa gtatgcccga tttattgata atgatcaggt cgtcggaatc
     1561 gctcacctct ttaattgcaa tcaggcttcc ggttttttgc gtgatattga tcgttttaac
     1621 acctttgccc ccacggttgg ttatcctgta gtcggccagg ctcgaccttt tgccgtatcc
     1681 gttctccgat acaaccaaca cgtcaaaatt ctcggtttcc acgcatatca taccaataac
     1741 ctcatcgttg ctatttcctg gggttatacc cttgacaccg gcagcatttc gacccattgg
     1801 cctgacattg ctttcattaa accggatggc gcgcccactc ctcaaagcca gcatcacttc
     1861 attggtgccg ttggtcagct tggctgccag caactggtcg ccttcgttga ctgtgatagc
     1921 gataataccg gcctgccgtg gcctcgaaaa cgcctccagc gttgtttttt tgatgattcc
     1981 gcgttgggtg caaagtatta tatagttgga agacaggtag ttctcatccg ttagatcttt
     2041 aatctttata aaggccctta ccttatcgtc ggtctctatg ttgatcaggt tctggatcgc
     2101 ccttcctttg ctgttcttcg ccccttcagg aatttcatag gtcctcaacc agaaacactt
     2161 ccccttttcg gtgaagagca gcatataatt atgggtatta gcgacaaata agtgttcgag
     2221 gaagtcctcc cctctcaggt cactgccccg ggctcccctc ccacccctgt tctgacgccg
     2281 gtattcggcc agtggcgtgc gttttatata gccaagatga gaaatggtaa tgaccacctc
     2341 ttcttccggg ataacatctt ccatccggaa gtcctctgcc gtatattcaa tcgtcgtacg
     2401 cggctcatcc ccgtatttat ccttgatttc ggtcagctca tccttaatga ttttcatccg
     2461 gagcgattcg tcggcaagga cctggcgtaa ataggcaatt tgtttctgaa gttcctcgta
     2521 ttcaagtttt attttatccc gctcaaggcc tgtcagacgt tgcagcctca tctcaaggat
     2581 ggctttggcc tgtatctccg taagctgaaa ttcacgcatc agtccatccc tggcttcatc
     2641 cggcgtccgg ctggcacgga tcagggcgat cacttcatcc aggtggtcta gagcaatcag
     2701 taatccttca aggatatgag cgcgtttttc agcctggtca agatcatacc ttgttcttct
     2761 gatcaccact tcatgcctgt gctcgacaaa gtagtgaatc agttccttca ggttgagggt
     2821 cttgggttta ccgtgaacga gggcaacatt gttgacattg aacgctgttt gcagggctgt
     2881 catctggtac agcttgttca atatcacgtt ggcaaccgca tctctcttca gttcgtaaac
     2941 aatccggagg ccgttccggt ccgattcgtc cctgatgtcg acaatgcctt caatcttctt
     3001 gtcattgatg atttctgccg tccgccggat catctctgct ttgttgacca tgtatgggat
     3061 actggtcaca atgatttttt ccttcccgcc gggtgtaggt tcgattttcg cttcgccccg
     3121 tacgacaatc cggccccggc cggtttcaaa agcttccctg acaccttcgt acccatatat
     3181 gatacccccg gttggaaaat caggtgcttt gatatattgc atcagctcct caatcgtgat
     3241 gtcgcggtta tcaataagag caatggtccc gtctatgacc tgggcaagat tgtgaggggg
     3301 catattggtt gccataccga cagcaatccc ggaagcgccg tttaccagca ggttcgggat
     3361 tttagccggc aatactttgg gctcct
//
LOCUS       DAOPNH010000326         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_33988_length_3357_cov_6.899455, whole genome shotgun sequence.
ACCESSION   DAOPNH010000326 DAOPNH010000000
VERSION     DAOPNH010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33988_length_3357_cov_6.899455"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2780)
                     /locus_tag="PKZ74_13440"
     CDS             complement(81..2780)
                     /locus_tag="PKZ74_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93805.1"
                     /translation="MRRIALIVILWVVFLLFAFKLSAQYIEVSQPEINGPVFIGYFDG
                     ERMQKRVVSDYKTNPMARLDIKGEIVNLDLTVFRDGYENRVSNPSREAIQWLEPGAPV
                     EGDLHSDIAYTRDGTMFAVIYQNSDNMTFFDAQTCEPIETVQLVRQPVDLYMGEQNAY
                     VACHEGRSIVAVSLEDFSIVNYIPVDGYPCQVEVSPGEDTAFIACDSYRDGWMIAVDM
                     ANNNEVLYLTHDPYFHHYGWGGAMGRTLYKFSKFHLSPKGGQFICGDTSTYQPTIFNT
                     SNGHPVKTFWIGGWRGAGYSPTGDTIYIYSNNEDTVKMLRVNADDFTVIDAIYSDIGC
                     MIGFVSYTDLAISHDGNEVLVSDRANDRYCRFDFDDYTCQVITPWILNEDAHIFSSSD
                     GQYAVVANYLKLQIVNLETGALNDNWPSSMRVGSPLCVSPFENTLVVGNSPGMGIEKL
                     WVVDFSNVNTLIQEASITCGEPPEADMPHVAAVSENGQKAFSVNYLTRDISIIDIYNN
                     TLDTLIYCEDISGLKLIPRTDYALIYGEQKVFSHIVSQLDFSEVAEIQAGGIRDALVS
                     SDGQMAFLLEYQVYDWMRITKVRIDGPGSFIEKQIMSLGCDCGISFTNDEVDIETRPA
                     LSPDDKMLLIGDDNDTLGPVVNIIDTETLEILCQVPVLDECVYDFAFTDDSRRVAVVN
                     FYNSLIPIIYLDGENSFVESYIPIATRSYGAAYNKMDGLFYILDQTNYIFKADPLTGE
                     VVGQMNTFDDTNWDIEIDLRGNPLVLTNYSMIYSGEAYAMPGYTSILSYDAEHDLFIS
                     PVPGPDKVCIFDPALVGIKTLNPADQTCIKIFPNPSADDFILESDDKILQVEIFTLKG
                     DMVFRKEFDDKTAVIPSKSLKPGIYLFRITTESGIFSRKAIVS"
     gene            complement(2853..>3357)
                     /locus_tag="PKZ74_13445"
     CDS             complement(2853..>3357)
                     /locus_tag="PKZ74_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93806.1"
                     /translation="AVSFFNIDDDREDSLIFVTLNGFEEDKIAGWSSVKEELKCKDEF
                     SGEISVQVNAESFSPGFAMNLDSIPFGDLRFATDARLSAPGYKFKNKILPVISVETEK
                     GTILWQSIPADLQLIDEKGWNHIYNQALYYHSTPGCTLKIYFWNQSDIPLLLDDFRVM
                     VFLPATS"
BASE COUNT          920 a          832 c          761 g          844 t
ORIGIN      
        1 ctttagtcgt tggagcacga tcactggcaa ccaacggatt cacccgtcgg actaccctcc
       61 tttaaatgca tcataaagtc tcagcttaca attgccttcc tcgaaaaaat acctgattcc
      121 gttgtgatcc tgaataagta aattcccggt tttaacgatt ttgaaggaat aacggctgtt
      181 ttatcatcga actccttcct gaaaaccata tctcctttta aagtaaagat ttctacctgt
      241 aaaattttat cgtctgattc caggataaaa tcatcggcgg aaggattcgg aaatatcttt
      301 atgcaggtct gatcagccgg attcaatgtt ttaataccga ccaaagccgg atcaaaaatg
      361 cagactttat cggggcccgg aaccgggctg ataaacagat catgttcggc atcatagctg
      421 aggatggagg tatacccggg cattgcgtaa gcttcgccgg aataaatcat ggagtaatta
      481 gtgagaacca gcgggttgcc gcgaagatcg atctcaatgt cccagttggt atcgtcgaaa
      541 gtattcatct ggccgacaac ctcacccgtc aggggatcgg ccttgaagat ataattcgtt
      601 tgatcgagga tatagaacag cccgtccatt ttgttgtacg ccgcgccata gctccgggtg
      661 gcaatcggta tataactctc cacaaacgaa ttctctccat caaggtaaat gatcgggatc
      721 aaggaattgt aaaagttaac caccgccacc cttctggagt cgtcggtaaa ggcgaaatca
      781 tagacacatt cgtccaaaac cgggacctgg caaaggattt caagggtttc cgtatcgatg
      841 atgttcacta ccgggcccag ggtgtcgttg tcgtcgccga tcaggagcat tttatcgtcg
      901 ggactcaggg caggccgggt ttcgatatcg acctcatcgt tggtgaaact tatcccgcaa
      961 tcacatccca ggctcatgat ctgcttttca ataaagctgc cgggcccatc gatcctaact
     1021 ttagtgatcc tcatccagtc atacacctgg tattccagaa ggaacgccat ctggccatcg
     1081 gaagaaacca gcgcatccct gattcctcct gcctgtattt cagcaacttc cgagaaatcg
     1141 agctgggaga caatatgact gaacaccttt tgttcgccat agatgagggc ataatcggtt
     1201 ctcggaataa gcttaaggcc cgagatatcc tcacaataga tcaatgtatc caaagtattg
     1261 ttataaatat cgatgatact gatatcccgt gtcaggtaat tgacggagaa agctttctgt
     1321 ccgttttcgg agactgccgc gacatgcggc atatcggctt ccggaggctc accgcaggta
     1381 atggaagctt cctggatcaa agtattcaca ttgctgaaat caacaaccca aagtttttca
     1441 atccccatgc cgggcgaatt accgaccacc agcgtatttt cgaatggcga tacgcaaagg
     1501 ggcgaaccca ctctcatact tgacggccaa ttgtcgttca acgcccccgt ttccaggtta
     1561 actatctgca atttcagata attagccaca acggcatatt gcccatccga agatgaaaat
     1621 atatgcgcat cctcattcag gatccaggga gtgataacct ggcaggtgta atcgtcaaag
     1681 tcaaaccggc agtaccggtc gtttgccctg tcgctgacca gcacttcgtt gccatcgtgg
     1741 ctgatcgcca ggtcggtgta actgacaaag ccgatcatgc agccgatatc gctgtaaata
     1801 gcgtcaatta ccgtaaaatc atctgcattg accctcagca tcttaaccgt gtcttcatta
     1861 ttggaataaa tgtaaatcgt atcgccggtg ggggaatagc ccgccccgcg ccagccgccg
     1921 atccagaatg tctttaccgg atggccgttt gatgtattaa aaatggtggg ctggtatgtg
     1981 gacgtatcgc cgcagataaa ctgtccgcct ttgggtgaaa ggtgaaattt actgaattta
     2041 tagagggtcc ggcccatggc gccgccccag ccataatggt gaaaataggg atcgtgggta
     2101 aggtacaaaa cctcgttgtt attggccata tcgacggcga tcatccagcc gtcgcggtaa
     2161 ctgtcgcacg caataaaggc cgtgtcttcc cccgggctca cctcaacctg gcagggatag
     2221 ccgtcgacgg gaatataatt gacaatgctg aaatcctcca gggagacagc aacgatactg
     2281 cggccttcat ggcaagccac ataagcattt tgttcaccca tatacaggtc gaccggctgc
     2341 cggaccaact gcaccgtttc gatcggctcg caggtctggg catcaaaaaa agtcatgtta
     2401 tcgctgttct ggtaaattac ggcaaacatt gttccgtcgc gggtataggc gatatcggag
     2461 tgaaggtcgc cttcgaccgg cgcaccgggc tcgagccatt gaatggcttc ccgggaaggg
     2521 ttggaaaccc tgttctcata accgtccctg aagacggtca agtctaaatt gacaatttca
     2581 cccttaatat cgagccgtgc catcgggttc gttttgtaat cgctgacaac ccgcttttgc
     2641 atgcgttcac cgtcaaagta accgataaaa accggcccgt ttatctccgg ttgagaaact
     2701 tcgatatact gagcggaaag tttaaaggcg aaaagaagaa agactaccca gagaatgaca
     2761 attaatgcga tgcgtctcat aaccaggatt tttaaagcgc aacaaattta aatattatta
     2821 atgtcaatga caaataagta aagaataact tatcaggagg tcgccggaag aaacaccatc
     2881 acccggaaat catccaggag cagcgggatg tcgctttgat tccagaaata gattttcagg
     2941 gtgcagccgg gagtgctgtg ataatataaa gcctggttat atatatggtt ccatcccttt
     3001 tcatcaatta gctgaagatc ggccgggatg ctttgccaga ggatggttcc tttctccgtt
     3061 tcaaccgaaa tcaccggcaa gatcttattc ttaaatttat aacccggagc actgagccgg
     3121 gcatccgttg caaatctcaa atcaccaaag ggaatgctgt cgagattcat cgcaaacccg
     3181 ggagaaaacg attctgcgtt tacctgaaca gaaatctctc ccgaaaactc atccttacat
     3241 ttaagctctt ctttaacaga actccatccg gctattttat cttcttcaaa accgttcagg
     3301 gtaacgaaaa tcaaactatc ctcccgatca tcgtctatat taaaaaaact cacagcc
//
LOCUS       DAOPNH010000327         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_34139_length_3346_cov_7.880583, whole genome shotgun sequence.
ACCESSION   DAOPNH010000327 DAOPNH010000000
VERSION     DAOPNH010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34139_length_3346_cov_7.880583"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKZ74_13450"
     CDS             <1..984
                     /locus_tag="PKZ74_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM93807.1"
                     /translation="GWVDQAAGIINLAWNSSSPLTFTQPETVAELVFTTKNPGQGELA
                     WYTGTTESYFTNASGNPIPADFHTGEVKIYEPPVIILDQSKTACVGQMITIPNSAIGN
                     QPPIDFLWIYPSGDTSIFYPVLFNTTIADAGIYTLLATDRVGCTDQKSIELIVSENPV
                     ASFHGTDTLEMQAGDVLDAGAGLSAYLWNTGDSTESIVINAEGMYSVEMKSPVGCLGS
                     DSVYVKLTSEEIPEFAIYVPNAFSPNGDGINDIFKIKFPNSTFNIQHLTLSIFDRWGG
                     MVYTTDDVAVGWDGKKNGKNCPGGVYVYKIVFSVDSVPGNQERVGTVMLVR"
     gene            1311..>3346
                     /locus_tag="PKZ74_13455"
     CDS             1311..>3346
                     /locus_tag="PKZ74_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93808.1"
                     /translation="MLFLSTSLFAQNEANIWYFGVHCGLDFNTGEPVALHDGQTATYH
                     FGYSTICDSSGELLFYMDYSFVFNKIHEIMQGGTDLVNPSVGGRAIVKWPGQDGMYYI
                     FSPGYAGHDAFSYSVVDMKLDGGKGSITEKNVPVEAALDASGRVATVRQTNTDNVWVI
                     TRKFLHDAMVAFLVDEDGFDSIPVSCDMPDQVGNDFGNWGYIKVSYDKKYIVSTYQGG
                     SGGDFEFCRFNASNGSAEYMYSLNSMNGRIAPRGVEFSPDSKFMYVAFGGWDSVDVYQ
                     FPMSLIENPGLFSISGILVGTGAAWTLQLARDGMIYCGNMLDYPYYSMNVISKPWISG
                     PGCSFEMCNVSTYPGELEEQFNNILVDYLLRFEWTGEQCQGYPIQFKPNFIPTPQTIQ
                     WDFDDGPGSNSWQLSPAYAFKNPGLHEVKVDVWYPSGRYEHTSREIEISPSPQPDLGP
                     DTLICNGASITLNANCEADFFSWSSGQFGVSSITVSDSGTYRVRCRFNESGCMGYDTI
                     HVGFHLPTLIDETNLVIIPTTCNGASGSITGLTALGSTPFAYQWRDLSGNDYGVNIDA
                     SGLPAGQYYLTIIDGNGCETESPVYTIEDAGNLQVLNVELTQPHCGRPDGQVFVHAFS
                     PSGSTLEYSINDGADYSSDSIFTGLSGGIYVVHIRDVNGCEGFYLANPVILADIP"
BASE COUNT          851 a          792 c          771 g          932 t
ORIGIN      
        1 ggctgggtcg accaggcggc aggaataatc aatctggcgt ggaattcatc ctcacctttg
       61 accttcaccc aacctgaaac tgtcgctgaa ctcgtattca caaccaaaaa tcccggacag
      121 ggtgaattgg cctggtacac cggaaccacg gagagctatt ttaccaacgc aagcggcaat
      181 cccattccgg ctgactttca tactggcgaa gtcaaaattt acgaaccacc agtcatcatc
      241 ctggatcaaa gcaaaaccgc ctgtgtgggg cagatgataa ctattccgaa ttcggctatc
      301 ggcaatcagc caccaattga tttcctttgg atttatccat caggagatac ttccattttc
      361 taccccgttt tatttaacac gacaattgct gatgccggca tttacactct tctcgccacc
      421 gaccgtgtcg gctgcaccga tcagaaaagc atcgagctga tcgtcagcga aaatccggtt
      481 gcatcctttc acggcaccga tacccttgaa atgcaggcag gtgatgttct tgatgcagga
      541 gcaggcttat ctgcttatct atggaatacg ggcgacagca cggaaagtat tgtcatcaat
      601 gctgaaggaa tgtactcggt cgagatgaag tctcctgtgg gttgcttggg ttcagattcc
      661 gtttatgtaa aactcacaag cgaagaaata ccagagtttg caatttatgt tccgaatgcg
      721 tttagtccga acggtgacgg cataaacgac atttttaaaa taaaattccc gaattcaaca
      781 ttcaacattc aacatttaac cttaagcatt tttgatcggt ggggtgggat ggtttataca
      841 actgacgatg ttgcggttgg ctgggatggc aagaaaaacg gaaagaattg cccaggaggg
      901 gtgtatgttt acaaaatcgt tttctcggtt gatagtgttc cggggaacca ggagagggtc
      961 gggacggtga tgctggtgcg gtaaaattcc aaatccaaaa ttcaaaatcc attgtaatat
     1021 cgccggatcg cctgatcgtg ttttcgcctg attattttgt ccaactgtcc aactgtccaa
     1081 ccgtccaact gaaattctct tgtttttctc cccaaaacat cctactttta tcccacccat
     1141 ttcatccgca acgatcttca taatgatgta aaatgaaaac cctgaaatta cacttacact
     1201 ttcctaaatt cctacagtct tgccgtctta ccgtcctaaa gtcctacagt cttacagtcc
     1261 tcaagtccta ctgtcatacc gtcttacagt cctaccgtct tacagtcctg ttgctcttcc
     1321 tctccacctc cctcttcgcc cagaatgaag ccaatatctg gtactttggc gtgcattgcg
     1381 gtctggattt taatacgggt gaaccggtag cgcttcatga tggtcaaacc gcaacatatc
     1441 atttcggtta ttcaaccatt tgcgattctt ccggcgagtt attattctac atggattact
     1501 cctttgtttt taataagatt catgagatca tgcagggggg aacagacctg gtcaatccga
     1561 gtgttggggg aagagccatt gtcaaatggc ctggacagga tgggatgtac tatatttttt
     1621 cgcccggtta tgcaggacat gatgcttttt catactccgt cgtcgacatg aaacttgacg
     1681 ggggtaaagg atccattaca gaaaaaaatg tacctgtcga agcggcgctc gatgcttcgg
     1741 gcagggttgc cactgtgaga caaaccaata ctgataatgt atgggtgatc acccggaaat
     1801 ttcttcatga tgccatggtc gctttcctgg tggacgaaga tgggtttgat tccatcccgg
     1861 tctcctgtga catgcctgac caggtaggaa atgattttgg taactgggga tatattaaag
     1921 tttcgtatga taaaaagtac attgtgtcaa cttaccaggg aggttcggga ggagattttg
     1981 aattttgccg ctttaatgcc tccaatggaa gcgctgaata catgtattca ttgaatagca
     2041 tgaacggacg aattgctcca cggggagttg aattttcgcc ggattcgaaa tttatgtacg
     2101 ttgcttttgg gggttgggat tcggttgatg tttatcagtt tcccatgagt ttgatcgaga
     2161 atccgggctt gttcagcatt tcgggcatcc tggttggcac aggagctgca tggaccctgc
     2221 aacttgcaag ggatggaatg atttattgcg gaaatatgct ggactatcct tattattcta
     2281 tgaatgtgat cagcaaaccc tggataagcg ggccgggatg ctcctttgag atgtgtaacg
     2341 taagcacata cccgggtgaa ttagaggaac aatttaataa catcctcgtg gactatcttc
     2401 tccgcttcga gtggaccggc gaacagtgcc agggatatcc aatccagttt aagccgaact
     2461 tcattccaac tccacaaact atccagtggg atttcgatga cggaccggga agcaactcat
     2521 ggcagctttc accggcctat gcttttaaaa acccaggctt gcatgaagtg aaggttgatg
     2581 tctggtatcc ttccgggaga tatgagcata cttcaagaga gatagaaatc tccccatcgc
     2641 ctcaacccga tcttggtccg gatacactca tttgcaacgg cgcttccatt acgctcaatg
     2701 ctaattgcga agccgatttc ttttcctggt ccagcggaca attcggcgtt tcgtcaatta
     2761 ccgtttctga tagcggaact taccgggttc gatgtcgttt caacgaatcc ggctgcatgg
     2821 gttatgacac gatccatgtg ggcttccacc taccaactct catcgacgaa accaatcttg
     2881 ttataatccc caccacctgc aacggagcaa gcggttcaat aaccggactc actgctcttg
     2941 gctctacgcc cttcgcatat caatggcgcg acctgtcagg aaacgattac ggcgtgaaca
     3001 tcgatgcatc gggcctcccc gcagggcaat attacctgac aattattgat ggcaacggtt
     3061 gcgaaacgga atctccggtt tacaccatcg aagatgccgg caatctgcag gttttaaatg
     3121 ttgaactcac tcaaccacac tgtggaaggc cggatggcca ggtctttgtg catgcttttt
     3181 ctccttcagg atcaacactt gaatattcta tcaatgatgg agcggactat tcatcggata
     3241 gcattttcac cggattatct ggcggtattt acgtcgtcca tatcagggat gtcaatggct
     3301 gcgaaggatt ttatcttgca aacccggtta tcctggccga tattcc
//
LOCUS       DAOPNH010000328         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_34480_length_3325_cov_4.870642, whole genome shotgun sequence.
ACCESSION   DAOPNH010000328 DAOPNH010000000
VERSION     DAOPNH010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34480_length_3325_cov_4.870642"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKZ74_13460"
     CDS             complement(<1..261)
                     /locus_tag="PKZ74_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPM93809.1"
                     /translation="MDLSISIWIPLIPFFVFIFLGLAGHKFKPAFAGIVGTTGLLISF
                     ILSLLVAYQYFFVEGLQNGVYQQIIAFNSTWLRMSDTLVVSMG"
     gene            complement(295..612)
                     /gene="nuoK"
                     /locus_tag="PKZ74_13465"
     CDS             complement(295..612)
                     /gene="nuoK"
                     /locus_tag="PKZ74_13465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPM93810.1"
                     /translation="MIEGIPIVHFLIVTTIMFFVGVYGFLVRRNLITILMSLELMLNS
                     VAINFVVFNKYLYPGHLQGHFFSLFIIAVAAAEAAVAIAIIINVYRRFNNINVEDVSN
                     MKH"
     gene            complement(631..1140)
                     /locus_tag="PKZ74_13470"
     CDS             complement(631..1140)
                     /locus_tag="PKZ74_13470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPM93811.1"
                     /translation="MNILMFYLFASVIVIFSILTVTSRRILRSAVFLLFVLIATSAFY
                     FMLRYTFLAAAQLALYAGGIVVLIIFSILLTSHISERFPQITFKKAFFSALVAGAGAV
                     LSIVTLLQFDFDQQYDGGQTAELPVRDIGKSLMDFGEEGFVLPFEVISILLLAAMIGA
                     IVIAKKKKD"
     gene            complement(1142..1624)
                     /locus_tag="PKZ74_13475"
     CDS             complement(1142..1624)
                     /locus_tag="PKZ74_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM93812.1"
                     /translation="MKGIVNYIREIIQGVITLIKGMAVTGKYFVTSPKTIVTQKYPEN
                     RKELEMFDRFKGEVIMPHNENNEHRCTGCGICEMNCPNGSIEIITKTEEIDGKKKRVI
                     DKHIYHLSMCTMCALCVKTCPSNALAFGQTFEHAVYDRKQLTKVLNKPGSVLTKEIKE
                     "
     gene            complement(1661..2671)
                     /gene="nuoH"
                     /locus_tag="PKZ74_13480"
                     /pseudo
     CDS             complement(1661..2671)
                     /gene="nuoH"
                     /locus_tag="PKZ74_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
     gene            complement(2768..>3325)
                     /locus_tag="PKZ74_13485"
     CDS             complement(2768..>3325)
                     /locus_tag="PKZ74_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPM93813.1"
                     /translation="RLPEYDQLLTGNVIFQERTKGIGRLTREEAVNYGVTGPSGRGSG
                     FSCDIRKKFPYSAYPYVDFKEILGVDGDALSRYKVRMEEMWESMHIIEQLIDNIPEGP
                     YTAKMKAVLKLPAGEYFQRVETARGELGVYVISDGNKTPYRMKFRSPNMCNLSALDHI
                     SRGAKIADLVAIGASFDFVIPDIDR"
BASE COUNT          860 a          822 c          848 g          795 t
ORIGIN      
        1 tcccatgctg accaccaacg tgtctgacat cctgagccag gtgctgttga aggcaataat
       61 ttgctgataa acgccatttt gcaggccttc gacgaaaaag tactggtatg ctaccagaag
      121 tgaaaggata aaggagatga gaagtcctgt cgttccgact atcccggcaa aggcgggttt
      181 gaatttatga ccggccagtc ccaggaatat aaaaacgaaa aatgggatga gcggtatcca
      241 gatgcttatg cttaaatcca taactttctt ggtttcagtt ttgattcatt ccttttaatg
      301 cttcatgttg ctgacatctt caacatttat gttgttgaac cgcctgtaaa cattgatgat
      361 gatggcgatg gccactgccg cttccgctgc cgccaccgcg atgatgaaca gggaaaagaa
      421 atgtccctgc aagtgccccg ggtagaggta tttattgaaa acgacaaaat tgatggcgac
      481 cgagttcagc atcagttcca gcgacatcag gatggtgatt aggttgcggc gcaccaggaa
      541 gccataaacc ccgacaaaga acatgatcgt ggtgacgatc aggaaatgga cgattggaat
      601 gccttctatc atgatgattg gttttacagg ttaatctttt ttcttttttg cgatgacgat
      661 tgctcctatc attgcggcca gaagcaggat ggaaatgacc tcaaacggca ggacaaatcc
      721 ttcttccccg aaatccatca ggctctttcc gatatccctg acaggtaatt cggcggtctg
      781 gccgccgtcg tactgctgat cgaagtcgaa ctgaagcaag gtgactatgc tcagaaccgc
      841 cccggcaccg gctaccaggg ctgagaaaaa ggccttttta aaggtgattt gcggaaaccg
      901 ctcgctgata tggctcgtca gaagaatgga aaagatgatc agaacaacga tgccgcccgc
      961 atacagtgcc agctgagccg ctgccaggaa agtgtaccgc agcatgaaat aaaatgccga
     1021 ggtggcgatc agcacaaaca aaaggaaaac ggctgaacgg agaatcctcc ggctcgtaac
     1081 ggtcaggatc gaaaaaatga cgatcacgct cgcgaaaaga tagaacatca atatattcat
     1141 actattcttt gatttctttg gttaatactg agccgggttt gttcagcact ttcgtcagtt
     1201 gcttccggtc ataaacggca tgctcgaatg tctgcccgaa ggcaagggca ttggaagggc
     1261 atgttttaac acataaggcg cacatggtgc acatggagag atgataaatg tgcttgtcga
     1321 tcacccgctt cttcttaccg tcgatttcct cggttttagt gatgatctcg atagagccgt
     1381 tggggcagtt catttcgcag attccgcaac cggtgcaacg gtgctcgttg ttttcgttgt
     1441 gtggcatgat gacttcccct ttgaaccggt cgaacatttc gagttctttc cggttctcag
     1501 ggtatttctg cgtgacgatg gtcttggggc tggtgacaaa atatttgccg gtcaccgcca
     1561 tgcctttgat cagggtgatc accccctgga ttatctctct tatataattg acaatgcctt
     1621 tcatatgttc atttttacgt tgaaagatca gctctaccgg ttagaaatgc catttcatca
     1681 tggtcaggaa agcgatcagg accgtattca ccaggttgat cggcagcaaa tatttccatt
     1741 cgagcgtcag aagctggtcg atcctcaacc gggggaaggt ccatttgaac cacatcatta
     1801 aaaagatcac gaaagcggtc tttcccatat accatactac cggcgggata tagtccatta
     1861 cccggttaaa ggcgtcaaaa ccccagaagt gcaggggcat ccagcctccc aggaaaaccg
     1921 tggcggcgat ggaagcgaca atgaacatgt tcatgtattc ggccaggaag aagaaggcga
     1981 atttgatccc ggagtattcg gtgtggtagc cggccgtcag ttccgattcg gcttcggcca
     2041 ggtcaaaggg tccgcggtta gtttcggcag tggctgcgat caggaagatc acaaaggaga
     2101 tgaccgccgg gatgtgccct ttgaagatcc accaggcatc ggcctggctc tcgacgatct
     2161 ctgagaattg cagcgtgcct gaaaagataa cgaccgaaag cagcgccagg ccgaccgaca
     2221 gctcgtagct gacaatctgc gcaccgctcc ggatggcccc gatcagcgaa tacttgttgt
     2281 tgctcgacca tcccgcaagg agaataccca caacacccat ggatgatacc gccatcacat
     2341 aaaaaacgcc tatgttaaaa tcgatggcat ggagcccttt tgcaaatgga attgcagcga
     2401 tggccatgaa ggaggcgatg atgacgataa acggagccag gttgaacagg aacttgtcgg
     2461 cctccttgat ctttaccagc tctttcatca acagcttgat aaagtcggcg atggtctgca
     2521 gcaatcccca ggggccgacc cggttgggtc ccagccggtt ttgcatgaag gcacagacac
     2581 gcctctcggc atagaccagg atcaggccgg taacggcata aaaaatcagg aataccaggc
     2641 cgatgatgac catctcccag gcaatggccc agaatggggt gaacgtttcc tggagccagg
     2701 cttcgatgcc ggcggtcagg gaagtgaaat cgtataacca gtgatctttc ataaggtaat
     2761 gcgaatatta gcggtcaata tctggaataa caaaatcaaa ggaagccccg atggccacca
     2821 ggtcggcaat ctttgcgccg cgcgagatgt ggtcgagggc cgaaaggttg cacatattcg
     2881 gcgagcggaa tttcatccgg taaggggtct tgtttccatc gctgatgacg tacacgccaa
     2941 gctctccacg ggctgtttcc acccgctgga agtactcgcc ggcaggcagt ttcagcaccg
     3001 ccttcatctt ggcggtataa ggcccttccg ggatattatc gataagctgc tcgatgatgt
     3061 gcatcgattc ccacatctcc tccatcctca ccttgtagcg gctcagcgca tctccatcca
     3121 ctccgaggat ctctttgaaa tcaacataag gataagcact gtaagggaat tttttacgga
     3181 tatcgcatga aaagcccgaa ccgcgaccgg aaggccctgt tacaccataa tttacagctt
     3241 cttcccgcgt cagtctgccg attcctttgg tccgttcctg gaaaatgaca ttgcctgtca
     3301 gcaactggtc gtattcagga agcct
//
LOCUS       DAOPNH010000329         3307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_34753_length_3307_cov_6.974477, whole genome shotgun sequence.
ACCESSION   DAOPNH010000329 DAOPNH010000000
VERSION     DAOPNH010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34753_length_3307_cov_6.974477"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKZ74_13490"
     CDS             complement(<1..301)
                     /locus_tag="PKZ74_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93814.1"
                     /translation="MKSRIEFAQQELSKRNLYNSIIDGLLSADTLAALDALPLSYTRI
                     KPEWEALRKVYGFIQIACLEAGFDPGEIDGRWGPNTENAFNNYRYLLANGKPPAPW"
     gene            complement(345..1223)
                     /locus_tag="PKZ74_13495"
     CDS             complement(345..1223)
                     /locus_tag="PKZ74_13495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM93815.1"
                     /translation="MKKLFKAFRAIGLILRNPWLLNKILEDDQFWKKKINRDLGRKDV
                     LPVIGPDSLSGEFSAFIQPFAFGDGGSLPTDLSLLVKIAEMIPGCKYFEIGTWRGESV
                     ANVARVAKECYTLNLSDEEMLAMDLPLDYIAQHRVFSLGLSNVVHLQGNTKSFDFASL
                     NMKFDLIFIDGDHHFEMVKNDTQKVFQHLVHDKTIVVWHDYARSPETVRWEVLAGILA
                     GTPKEIQHHIYHVAHTLCAVYLPGFEKPDYLRPDLVKDFFQVEIKYFNETPSRKFEME
                     SHNPQTPSQPDQMESH"
     gene            1395..2042
                     /locus_tag="PKZ74_13500"
     CDS             1395..2042
                     /locus_tag="PKZ74_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM93816.1"
                     /translation="MESQKLLLIQKLNTMVSKKLKSPVCPDRYYHIYNRGNNKEKLFY
                     HSGDYHFFLEKYKQYVTPYADTYAFCLLPNHFHFLIKTGEKTNASISAVSNQLRRLFI
                     SYTKRINNIHNRSGSLFTKNYQRIEIDSDRYLTQVIRYIHKNPVKHNIQNDYENYHYS
                     SYRIIVSDHETELKRNEVIDWFINKKEFIDYHRNDQVDPTTFNLLSIEDENSPDG"
     gene            2206..>3307
                     /locus_tag="PKZ74_13505"
     CDS             2206..>3307
                     /locus_tag="PKZ74_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM93817.1"
                     /translation="MVASEKYTEDSIKSLDWREHIRLRPGMYIGKLGDGAAQDDGIYV
                     LMKEILDNCIDEHVMGFGSRIDVAIKEKTVVIRDYGRGMPLGKVIDCVSKINTGAKYD
                     SKVFKKAVGLNGVGSKAVNALSSYFKAQSVREGKTKIVEFERGDLIRDHEIAPCDLPG
                     GTEITFIPDDTIFGHYYYITEYVEKLLWNYAFLNNGLILTLNGEKFQAKNGLLDLLSL
                     YLNGNGGALYPTIHIKEEEFECAFTHSSKQYGEEYYSFVNGQYTPQGGTHLQSFREAI
                     VKTIREFYNKDYDTNDIRASIIAAISLKVQEPIFESQTKTKLGSQHMEPEGQTIRNYI
                     MDMVKKHLDNYLHKNPETAEALQLKILQSEKER"
BASE COUNT         1001 a          745 c          710 g          851 t
ORIGIN      
        1 gccatggagc tggcggttta ccgttggcca gtaggtagcg gtagttgtta aatgcattct
       61 cggtgttggg cccccagcgg ccgtcgatct ctcccggatc gaagccggct tccaggcagg
      121 ctatctggat aaatccgtaa actttgcgaa gcgcttccca ttccggtttg atccgggtat
      181 atgacaaagg caaagcatcg agggcagcca gcgtatcggc acttagtaat ccgtcaatta
      241 tggagttgta aagatttcgt ttcgaaagtt cctgctgggc gaattcgatc cttgatttca
      301 tggggtactg gtttttagat gtttaagtga gactccatct cgatttaatg agactccatc
      361 tggtcgggtt gagatggagt ctggggattg tgagactcca tctcaaattt acgagatgga
      421 gtctcgttaa aatacttgat ctcaacctgg aaaaaatctt taaccaaatc aggtcgtaaa
      481 tagtctggct tttcaaatcc gggaagatag acagcacata aagtatgagc aacatgataa
      541 atatgatgtt ggatttcttt cggggtgccg gccagtatcc cggcaagtac ttcccatctg
      601 actgtttccg ggctgcgcgc ataatcatgc cagaccacga tggttttatc atgtaccagg
      661 tgttgaaaca ctttttgtgt atcattctta accatttcga agtgatggtc accatcaata
      721 aatataagat caaatttcat gttcagtgac gcaaaatcaa atgattttgt atttccctga
      781 aggtgaacga cattcgaaag ccccagggaa aatacccggt gttgtgcgat ataatccaaa
      841 ggtaaatcca ttgcaagcat ctcttcatcc gacaggttca acgtgtagca ttccttagcc
      901 acccgtgcaa cattggcaac actttcgccg cgccatgtgc caatttcgaa gtatttgcag
      961 ccgggaatca tttcggcaat ttttaccagg agggagaggt ctgtcggtag cgagcccccg
     1021 tcgccgaatg caaaaggttg aataaatgcc gagaactctc cggaaagtga atcaggaccg
     1081 attacgggca atacatcctt tcgcccaaga tcccggttta tttttttctt ccagaattga
     1141 tcatcctcca ggattttgtt cagaagccag ggatttcgca aaatcaatcc aatggcgcga
     1201 aaagctttga ataatttttt caaattgatg aattctttac caaatttata aaaatttaca
     1261 actcaccaaa tcgagactcc atctcaattt atccagatgg agtctcaaat attttcaacc
     1321 gaccgggtat ttttcatcgc ttttccggat taatagaata tgagggatgt gagactccat
     1381 ctcaaaaaaa ccagatggag tctcaaaaat tattattaat ccaaaaactt aacaccatgg
     1441 taagcaagaa attaaagtca ccggtttgcc cagacagata ctatcacatc tataacaggg
     1501 gcaataataa agagaaatta ttctaccatt caggcgacta ccacttcttt ttggagaagt
     1561 acaaacagta tgtaacacct tatgcagata catatgcatt ttgcctgtta cctaatcatt
     1621 tccatttcct gataaaaaca ggcgagaaaa caaatgcttc gatatcggcc gtatccaatc
     1681 aattaagaag gctttttatt tcatacacaa aaagaataaa taatattcat aaccgcagcg
     1741 gaagcctttt tacgaagaat tatcaaagaa tcgagattga tagtgatcgt tatctcaccc
     1801 aggtaattcg ctatattcat aaaaatccgg ttaagcacaa tatccagaat gattatgaga
     1861 attatcacta tagctcatac aggataatcg taagcgatca tgaaaccgaa ctcaaaagga
     1921 atgaggtgat cgattggttt attaacaaga aggaattcat tgattatcat cgcaatgacc
     1981 aggttgaccc tacaacgttt aacctattga gcatcgaaga tgaaaattct ccggatggtt
     2041 gagatggagt ctcatgattt tgagatggag tctcccttct ttgagactcc atctcaattt
     2101 atccagatgg agtctcaatt ccgattatca acaaaaaatt cttcataagt ttcatgtacc
     2161 gcccgtaaaa taaaggattc atgattgtac atttgcggct gaattatggt agcctcggaa
     2221 aaatataccg aagatagtat taaatcgctc gactggcggg aacatatccg gctcagaccc
     2281 ggtatgtaca ttggcaaact gggcgatggc gccgcacagg acgacggaat ctacgtcctg
     2341 atgaaggaaa tcctcgataa ttgcatcgac gagcacgtca tgggctttgg aagccggatc
     2401 gatgtggcga tcaaggaaaa aacagtcgtt atacgcgatt acggcagggg gatgcctctg
     2461 ggaaaggtga tcgactgcgt ttcgaaaatc aataccggcg ccaaatacga ctccaaagtc
     2521 tttaaaaaag cagtcgggct gaatggcgtg ggttcaaaag ctgtcaatgc gctgtcatcc
     2581 tacttcaaag cccaatctgt tagagaagga aaaaccaaga tcgttgaatt tgaacgcggc
     2641 gacctgatcc gcgaccatga aatcgccccg tgcgacttgc ccggcgggac cgaaattacg
     2701 ttcatccccg acgatactat tttcggacac tattattata tcacggaata tgtcgaaaaa
     2761 ctgctctgga attatgcatt tttgaataat ggactgatcc tcaccctcaa cggtgaaaaa
     2821 ttccaggcaa agaacgggct gctcgacctg ctcagcctct acctcaacgg caacggcggc
     2881 gctctctatc ctaccatcca catcaaagag gaagaattcg agtgcgcctt tacgcatagc
     2941 tccaagcaat acggcgaaga gtattattcc tttgtaaacg gacagtacac cccgcagggc
     3001 ggcacacacc tgcagtcgtt ccgcgaagcc atcgtcaaaa cgatccgcga gttctacaac
     3061 aaagattacg atacaaacga tatccgcgca tccatcatcg ccgccataag cctgaaggtg
     3121 caggaaccga tattcgaatc gcagaccaag accaagctgg gctcccagca tatggaaccg
     3181 gagggacaaa ccatccggaa ctatattatg gatatggtca aaaaacacct cgacaattac
     3241 cttcacaaga atcccgaaac ggccgaagcg cttcagctta agatcctgca atcggaaaaa
     3301 gagcgaa
//
LOCUS       DAOPNH010000330         3215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_36187_length_3215_cov_3.873734, whole genome shotgun sequence.
ACCESSION   DAOPNH010000330 DAOPNH010000000
VERSION     DAOPNH010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36187_length_3215_cov_3.873734"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PKZ74_13510"
     CDS             complement(<1..806)
                     /locus_tag="PKZ74_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPM93818.1"
                     /translation="MKKISTVICFIFIILAVPGCLLEDELKQSYTGFIPLEIGDGWDI
                     SSPAAENIDSAELENIYKAVYADDDLWMTKSLLVFRNGKLVSESYLKDDADRTRIDAV
                     WSCTKQVNAIITGIAIDDGHIAGVGATIEDYLPEYTSKYPDKAGITLENLLMMAGGIA
                     FDNGDQSDVFRTHSTENSLDFVLSLDLNFEPGDGFWYNDGEPQVVSGMVQAATGKPMD
                     EYAKEVFFNRIGMTNYQWVRYSDGVTLGGFGILTSPRELAKIAQCVLDSG"
     gene            complement(808..1560)
                     /locus_tag="PKZ74_13515"
     CDS             complement(808..1560)
                     /locus_tag="PKZ74_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93819.1"
                     /translation="MKRIAILVSCLMVLCGMTTAQTNHTHRHMLTINHLQVKESLNYG
                     LVFKGPGLGYMYSSLWENDRRVMEYTGVLSLNVPLTRGIISGSVNLVPARLDYRFKFD
                     PEGKVTLGPYVSLDYNMMIYPDLQSGYLFWLTNYSLGGVLSGWFSSKESRFDLCLHFT
                     VLGLTSRTKPYDNPYFFSGSFGELIGDLHSNLTFNPGQYTVNEIELRWTPKPSSRLAY
                     GYSFRYHGYFQEPELNMINHSVIIHFLPKKDK"
     gene            1713..2669
                     /locus_tag="PKZ74_13520"
     CDS             1713..2669
                     /locus_tag="PKZ74_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93820.1"
                     /translation="MKKTSMLFFAVLLSAVAYAQPVINKLYDFQPGDSYTFKKLAPGG
                     SIDYNSIETAGANLVWDLSFLQLEENPYTDTIIAYENSDWPNSFPGCNFVWKEYSGTE
                     QYYRKNGDSLLYMGNAVWAPSKFEPNPPTIVYPGNYTASGYMYSGFQSYLNSSALWTF
                     EARYNAYGTLVLPGNINVPNVALYSSYGGPSNSGYSDFMWVRENESLPIMRIQFYHSG
                     SSITVQYCYVLHAALTGIETIDETLVRAHVYPNPAKDFIYIESEQELSETKLTDLSGR
                     ILYQTKGSGNKVVIPAGDLTEGIYMVKFSDRNGRSAVTRIVH"
     gene            2936..>3215
                     /locus_tag="PKZ74_13525"
     CDS             2936..>3215
                     /locus_tag="PKZ74_13525"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM93821.1"
                     /translation="MSPKKKSLRIGILSSGGDCPGINAAIRGVGKTAIVEYGMEVIGI
                     SSGFLGLINEEYEILTEEKLSGILTLGGTILGTSREKPFKKDKEGGQDP"
BASE COUNT          894 a          771 c          691 g          859 t
ORIGIN      
        1 ttcccgctgt cgagcacaca ctgggctatt tttgccagct ccctcggcga ggtcaggatc
       61 ccgaatccac ccagggtgac gccatccgaa tacctgaccc attggtaatt ggtcatacct
      121 attctgttaa aaaacacctc ttttgcgtat tcatccatcg gtttgcccgt ggcggcctga
      181 accatgcccg agacaacctg cggctcgcca tcattgtacc agaatccatc gccaggctca
      241 aaattcaaat ccagggagag aacaaaatca agactgtttt cggttgaatg tgtcctgaac
      301 acatccgact gatctccatt gtcaaaagca atgccgccgg ccatcatcag caggttctcc
      361 agcgtgatgc cggctttgtc cggatatttc gaggtgtatt ccggcaggta atcttcaata
      421 gttgcaccga cgcccgcgat atggccatca tcaatagcaa ttccggtaat gatggcattt
      481 acctgcttag tgcatgacca taccgcgtcg atccgggtcc tgtcggcatc atctttcagg
      541 tagctctccg aaaccagttt gccgttacga aaaaccagca gactcttcgt catccacaaa
      601 tcgtcatcgg catagaccgc tttgtaaata ttctccagtt ccgctgaatc gatattctcc
      661 gctgccggcg atgaaatatc ccatccatcg ccgatttcca gggggataaa tcccgtatac
      721 gactgcttca gttcatcttc cagcaaacat cccgggaccg ccaggattat gaaaataaaa
      781 catattaccg tactgatctt tttcatctta tttatctttt ttcggaagaa aatgaattat
      841 gacagaatgg ttgatcatgt tcaactccgg ttcctgaaaa tatccgtgat accggaatga
      901 atacccgtag gccagccttg acgaaggttt cggagtccat cttagttcga tctcattgac
      961 ggtgtactgc ccgggattga acgtcagatt cgagtgcagg tcaccgatca gctcaccgaa
     1021 agaaccgctg aaaaaatagg gattatcata gggtttagtc cttgaagtga gtcccaaaac
     1081 agtaaaatga agacagagat cgaaccggct ttccttcgac gaaaaccatc ccgacaggac
     1141 acctcccagg ctatagttgg tcagccagaa aaggtaaccg ctctgcagat cgggatagat
     1201 catcatgtta taatcaaggc taacataggg cccgagcgtg accttgcctt ccggatcgaa
     1261 tttaaagcgg taatctaacc gggcaggtac caaatttacc gatcctgaaa taatgccgcg
     1321 ggtaagcggt acattcagcg acaatacgcc cgtatattcc atcaccctgc ggtcattttc
     1381 ccaaagggat gaatacatgt aacccaatcc gggtcctttg aagacgaggc cgtaattcaa
     1441 agattccttg acctgcaaat ggtttatggt tagcatgtgc cggtgagtat ggttcgtttg
     1501 cgccgtggtc atgccacaaa gcaccatcaa acaggaaacc agaatagcta ttcttttcat
     1561 ggtaaggtgt tgattctgtt ttggataata atggcacaag ttataaataa tattcacttc
     1621 aataaattaa aatgtaataa attaattttt atttaaattg attctaaatt gattattttt
     1681 gcgtaaaaca gcctaattaa tcagatcaaa ttatgaaaaa gacatctatg cttttctttg
     1741 ccgttctttt atcggctgtc gcatatgcac agcctgtcat taacaaactt tacgattttc
     1801 agcccggcga cagttatacc ttcaagaaac tggctccggg cggaagcatt gattacaaca
     1861 gtattgaaac tgccggggcc aatctcgtct gggacctgtc atttcttcag ctggaagaga
     1921 atccttacac agatacgatt attgcttatg aaaattcgga ctggccgaat tcttttccgg
     1981 gatgtaattt tgtatggaaa gaatacagcg ggacggagca atattacagg aaaaacggcg
     2041 attcgctcct gtacatgggc aatgccgttt gggcgccttc gaaatttgag ccaaaccctc
     2101 ccactatcgt ttatccggga aattacactg cctcaggtta tatgtattca ggcttccaat
     2161 cctatctgaa ttcttccgcg ctatggactt tcgaagcccg gtacaatgct tacggcaccc
     2221 tggtcctgcc gggcaatatt aatgttccga atgttgccct ttattcaagc tacggtggcc
     2281 cctccaattc gggttactcc gactttatgt gggttcggga gaatgaatcc ttacccataa
     2341 tgagaatcca gttctaccat tcaggatctt ccattaccgt tcaatactgt tatgtgcttc
     2401 atgccgcttt aaccggaatt gagacaatag atgaaaccct cgtccgggct catgtgtatc
     2461 ctaatcctgc taaagatttc atttacatcg aatctgaaca agaactcagt gaaacaaagc
     2521 ttacggattt gtcaggcagg attttatatc aaacaaaagg atcaggaaac aaggttgtta
     2581 tcccggcagg ggatctgaca gaaggtattt atatggtgaa gttttcggat cgaaacggaa
     2641 ggtcggcagt caccaggatc gtccattaac gaattcctgt tttgtttctc attgatttcg
     2701 agcacaaaat caaaggtcag tagaataagg ctgaaaagtt tattcattcg tgatcattcg
     2761 ataaaattcg tgtcattcgc gttctataat gcaacacgaa taaatacgga tgcaacgaat
     2821 acacgcgaat agcgcccgta gtttttcaat aacaagaata tcacatcaaa tctgtgaata
     2881 aaaactctgc cactcgttaa gaaaaaacct aatttcggtt ttcaaatttt ctcccatgtc
     2941 acctaaaaag aaatccctcc gaatcggcat cctcagctcc ggcggcgact gcccagggat
     3001 caatgccgcc atccgcggcg tcgggaagac cgctattgtc gaatacggga tggaagttat
     3061 cggcatctca tccgggtttc tcggcctgat caatgaagag tatgagatac tcaccgaaga
     3121 aaagctctcc ggcatcctga ccctgggcgg gactattctg gggacttccc gggaaaagcc
     3181 gtttaaaaag gataaggaag gcggacaaga cccgg
//
LOCUS       DAOPNH010000331         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_36222_length_3212_cov_5.206208, whole genome shotgun sequence.
ACCESSION   DAOPNH010000331 DAOPNH010000000
VERSION     DAOPNH010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36222_length_3212_cov_5.206208"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKZ74_13530"
     CDS             complement(<1..883)
                     /locus_tag="PKZ74_13530"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HPM93822.1"
                     /translation="MYDIIELNGKSLPELQDIAKALNVPKFEKLMKQDLIYQILDQQA
                     LIPVKDEKPEFNPEKPRRGRKKKVVTEEPKPPVQEEKPKEEVRQMEPRHKEKPVFSTE
                     PQQTQAEDAPREGMRKPKLVKKHERQQQGLFETRKDDRPGKNIESDLILPEIVNGDSD
                     EDLIPSPEAETEVTPESTVTVEKSEMDNFLEPKDEEVQPPLRDPGFRPRLNKKKDEFP
                     FEYEGFINSEGVLEIMPDGYGFLRSSDYNYLNSPDDIYVSQSQIKLFGLKTGDTVEGS
                     IRPPKEGEKYFPLIKVEM"
     gene            complement(1071..>3212)
                     /gene="gcvP"
                     /locus_tag="PKZ74_13535"
     CDS             complement(1071..>3212)
                     /gene="gcvP"
                     /locus_tag="PKZ74_13535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPM93823.1"
                     /translation="MSLVLLTPPGEWGADVVVGSSQRFGIPMGFGGPHAGFFATKEEF
                     KRNIPGRIIGISRDAAGNQAYRMALQTREQHIKRERATSNICTAQALLAIMAGMYGAY
                     HGQEGLRRIASKIHSLAQSLAEKASKAGFINENTLFFDTLKLALPSGKSAHDLLQVAN
                     HHEMNFRLIDEKHIGISLDETTSEKDILDILKVLAEFNGLKGGGDETVVWHERPKGIP
                     EPFLRKSKILQSPCFNRYHSETEMMRYMKKLENRDLALNRTMIPLGSCTMKLNAAAEM
                     FPLSWPEFGAVHPFVPAEQAEGYYRMVRDLEHDLSIITGFAGVSLQPNSGASGEYTGL
                     LVIRQYHISRGESHRNICLIPASAHGTNPASAVMAGMQVVVTKTDLSGNVDLDDLTEK
                     ATLHRDSLAAIMITYPSTHGVFEEHIREVIDVVHQNGGQVYMDGANMNAQVGYTNPGF
                     IGADVCHLNLHKTFAIPHGGGGPGIGPIAVAAHLKPFLPSHPVVRTGGDQGITAVSAA
                     PFGSALILPISYGYIKMLGKKGLKEATAYAILNANYIKARLEKHYKILYTGKNGWVGH
                     EMILDCNEFHKTADITVVDIAKRMMDYGFHAPTVAFPVHGTLMVEPTESEPLSELDRF
                     CDAMISIRNEIAEIESGTVDKTGNVIKNAPHTADMLTSDDWGFPYSRRKAAFPMANPS
                     QDKYFPPVTRIDDAYGDRNLVCTCNPVEDYQ"
BASE COUNT          724 a          802 c          753 g          933 t
ORIGIN      
        1 tcatctccac tttgatcagc gggaagtact tttcgccttc tttcggcggg cggatactac
       61 cttcaacggt atccccggtt ttcagcccga agagttttat ctgggattgt gatacatata
      121 tgtcgtcggg agaattcagg taattgtaat cggatgacct caggaaaccg tatccgtcgg
      181 gcatgatctc aagcactcct tccgaattga tgaatccttc atattcaaaa ggaaattcgt
      241 cttttttctt gttcagtctc ggtcggaaac caggatcccg cagaggaggc tgtacttctt
      301 cgtcttttgg ttccaaaaag ttgtccatct ccgatttttc aaccgtcaca gtagattctg
      361 gagtgacctc ggtttccgct tcgggtgacg ggatcaaatc ttcatccgaa tccccattca
      421 caatttcggg tagaataagg tcgctttcga tattttttcc aggccggtca tccttgcgtg
      481 tttcaaataa gccttgctgt tgcctttcgt gctttttcac cagttttggc tttctcatcc
      541 cttcacgcgg agcatcttcg gcctgtgttt gctgcggctc agtgctaaat accggctttt
      601 ccttatggcg aggctccatt tgacgaacct cctcttttgg tttttcttcc tgtaccggag
      661 gctttggctc ttctgtaacg acttttttct ttcttcccct gcgcggtttt tccggattga
      721 actccggctt ttcatcttta acagggatta atgcctgctg gtcgaggatc tggtaaatca
      781 gatcctgctt cataagtttt tcaaatttcg ggacattcaa cgccttcgca atgtcctgca
      841 attctggcag cgatttgcca ttcaattcaa tgatatcgta catttttatt tattataagt
      901 caatgatgaa acttttacca ggaaaaccaa ttgttgaaaa cgtaataccc agatgtaaaa
      961 gctgtagttt ctaatcctgt tataaaatgg gtttatgaga aataaacgga attctttcga
     1021 atgaggttgc aaatatacaa atatttctct taaacaataa ataaaaaaat ctattgataa
     1081 tcttcgacag gattgcaggt gcacaccagg ttgcggtccc cgtaagcgtc atcaatccgc
     1141 gtaacaggcg ggaaatattt gtcctgcgac ggattggcca tcgggaaagc cgcttttctc
     1201 ctggaataag gaaatcccca atcatcagag gtcagcatat ctgccgtatg aggggcattt
     1261 tttatgacat tgccggtttt atctaccgtt ccggattcaa tttcggcaat ttcattacgt
     1321 atgctgatca tggcatcaca gaaccggtcg agttcgctta gcggttcgct ctcagtgggt
     1381 tcaaccatca aagtgccatg taccgggaaa gcgactgtgg gtgcatggaa tccgtaatcc
     1441 atcattcttt tggcaatatc caccacggtg atatcggcag ttttatggaa ttcattgcag
     1501 tcaaggatca tttcatgacc cacccatccg ttcttgcccg tgtataaaat cttgtaatgc
     1561 ttttccagtc tggctttaat gtagttggcg ttcagaatgg cataggctgt tgcttctttc
     1621 aggccttttt taccgagcat ttttatatac ccgtaagata tgggaaggat aagtgcgctt
     1681 ccgaacgggg ctgcagacac agccgtgatg ccctggtcac ctccggttct gacaacaggg
     1741 tgggagggga ggaatggctt cagatgggcc gccacggcaa tcggtcctat gccagggcca
     1801 ccgccaccat gcgggatggc aaatgtcttg tgcaggttaa gatggcaaac gtcggccccg
     1861 atgaaacccg ggttggtata cccgacttgt gcgttcatat tggcgccgtc catatatact
     1921 tgtcctccat tttggtggac gacatcgatc acttccctga tatgttcctc aaaaacgccg
     1981 tgcgttgagg gataggtgat cattatcgct gcaaggctgt cacggtgtaa cgtggctttt
     2041 tctgtaaggt cgtccagatc aacatttccg gagagatctg tcttggtaac cactacctgc
     2101 attcctgcca tgacggcact ggccggattg gttccgtggg ccgatgccgg gatcaggcag
     2161 atattgcgat ggctttctcc tcggctgata tgatactgcc gaatgaccag cagaccggta
     2221 tactctccgg atgccccgga attgggctgt aacgataccc ccgcaaatcc ggtaatgatg
     2281 ctcagatcgt gctccaggtc ccgtaccatc ctgtaataac cctcggcctg ttcagcaggg
     2341 acaaagggat gtacggcccc gaattccggc cagctcaatg gaaacatttc ggcggccgca
     2401 ttcagcttca tcgtgcagga tcccagcggg atcatagtcc ggtttaaggc caggtcccgg
     2461 ttttccagct ttttcatgta gcgcatcatc tctgtttccg aatgataacg attgaaacaa
     2521 ggcgattgca aaatcttgct cttgcgcagg aatggctccg gaattccctt aggtctctcg
     2581 tgccaaacga cggtttcatc cccgccgccc ttgagcccgt tgaactctgc cagtactttg
     2641 agtatatcca ggatatcctt ttctgaagtt gtttcatcga ggctgattcc aatatgcttt
     2701 tcgtcgataa gcctgaaatt catttcatgg tgattcgcaa cttgaagcag gtcatgagcc
     2761 gactttccgg agggaagcgc cagtttgagt gtatcgaaaa agagcgtgtt ttcattgata
     2821 aaccctgctt tagaagcttt ctcagccagt gattgcgcga ggctgtgaat ctttgaggca
     2881 atccggcgaa gtccttcctg gccatgatat gcgccataca ttccggccat gatggccaga
     2941 agggcctggg ctgtgcaaat attggatgtt gccctttccc tcttgatatg ctgttcacgc
     3001 gtctgtaaag ccatacggta agcctggttt ccggcggcat cccttgaaat ccctataatt
     3061 cttcctggaa tattcctctt aaactcctct ttggtcgcaa aaaaaccggc gtgcggccct
     3121 ccaaaaccca tcgggatgcc gaaacgctgg ctggatccca ccaccacatc ggctccccat
     3181 tcccccggag gagtcagcag gacaagactc at
//
LOCUS       DAOPNH010000332         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_36596_length_3190_cov_7.663158, whole genome shotgun sequence.
ACCESSION   DAOPNH010000332 DAOPNH010000000
VERSION     DAOPNH010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36596_length_3190_cov_7.663158"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1560
                     /locus_tag="PKZ74_13540"
     CDS             82..1560
                     /locus_tag="PKZ74_13540"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HPM93824.1"
                     /translation="MLFRIFLDHQWKEYRRSPVWQRNMVANIFVGFFSFLMIVYLLMI
                     GFFLGPLLSEIMPDSDPVAIINGIILYFLGFDLLIRYLMQALPTFAIESYLHLPVRKS
                     SMVHFVISKSLFHLLNFLPLLVFIPFAARTILPSHGGAASVAWIVSILFLVISNNFLA
                     TYLKRILVSRPVITLLAGIVMIGLAAMDIYGLIRLSDISASAMGAVLKNPFLAILPVI
                     LAFLMYALNYFYLKSKVYPDEVIRHKTIQVQDIPRIKYLNSMGLTGDLIMLDIKLWWR
                     HKRTKTMLYMFPIFVLYGLFFYPNPIYRNDFSWLIFVGTFMTGGMTMNYLNYAFGYES
                     NHFDGLLTSRVNMDLYIRAKLTIGMLITTFCFIITIPYFLFSMDILLINFVAFLFNLG
                     FLSYLLLYMATFNKKRMDLAKGSSFNYQGVGLMNWLVLIPAFVLPLIIFAPFNLTGNR
                     YTGLAAIGLLGILGLISRRFWVKMIEKGFYARKYTMAEGFRE"
     gene            1773..2468
                     /locus_tag="PKZ74_13545"
     CDS             1773..2468
                     /locus_tag="PKZ74_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM93825.1"
                     /translation="MIGVSNLSKIYSGVTVLNIDSLEIKAGESFGLVGNNGAGKTTLF
                     RLILDLIRPQTGFVTSKEQDVSRSENWKLYTGSYLDRNFLIDFLTPEEYFDFNARIHG
                     MSPEDLQGFLESMTEFFNDEILGKKKFIRDLSKGNQQKIGVAAAFMADPEVIILDEPF
                     NGLDPSSQIRLINILNRLKENKRTTLLISSHDLNHVTEVCSRIAILEKGIIVKDLQTE
                     ENTLKELRNYFSV"
     gene            2490..>3190
                     /locus_tag="PKZ74_13550"
     CDS             2490..>3190
                     /locus_tag="PKZ74_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM93826.1"
                     /translation="MRNILLKSLLLISFLLAFQPDAMSQHPYSVSGRVIDAETREPLA
                     FVNIIQTGTKTGISTDIDGKFTLKSDRPIDAIWLTYVGYFPLQYSIPYGEVDKILIKL
                     QRKTFDLNEVVILPTANPAHRIIDSVLANRYRNDPEKLASFSYTSYEKTILTAKLDTM
                     MRVGKDSTGNWYSDTLIMEPDTAVMMTDTTLSDTALAEMKEFFDKQDIAIMESVVERK
                     FLAPDRNYENVKANRV"
BASE COUNT          772 a          805 c          753 g          860 t
ORIGIN      
        1 gtagtccgac gggtaaatcc gcgggttcca aagttagtgt aaaaatattt ttttactttg
       61 caggaagaaa actttcctgt tatgctgttc agaatattcc tggatcacca gtggaaagaa
      121 taccggcggt cgcctgtctg gcaacggaac atggtggcga acatcttcgt cgggttcttc
      181 tccttcctca tgatcgtcta cctgctgatg atcggttttt tcctcgggcc tttgctttct
      241 gaaataatgc ccgacagcga tcctgtcgcc ataataaacg gaatcatcct gtatttcctg
      301 ggtttcgacc tgctgatccg ctacctgatg caggcgcttc caacgtttgc catcgaatcg
      361 tacctccatc tcccggtcag gaaaagctcg atggtgcatt tcgttatttc caaatcgctg
      421 ttccacctgc ttaattttct gccgctgctg gtatttatcc cgtttgcagc caggaccatc
      481 ctcccgtccc acggcggagc tgcatctgtg gcatggatcg tttccatcct tttcctggtc
      541 atcagcaata actttctggc aacttacctg aagcggatcc ttgtctcccg cccggtaatc
      601 actttgctcg ccgggatagt aatgattggc cttgcggcga tggatatata cggtctgatc
      661 cggttatcgg atatttcagc ttccgccatg ggcgccgtcc tgaagaatcc cttcctggcc
      721 attctcccgg taattctggc ctttctaatg tatgcgctga attatttcta cctgaagtcg
      781 aaagtctatc ccgatgaggt gatccggcat aaaaccatcc aggtacagga tattccgcgt
      841 atcaagtatc tgaactccat gggattgaca ggcgacctga tcatgctcga catcaaattg
      901 tggtggaggc ataagcggac taagacgatg ctgtatatgt ttcccatttt cgtcctgtac
      961 gggttgtttt tttatccgaa cccgatttac aggaatgatt tctcctggct gatctttgtc
     1021 gggaccttta tgacgggagg gatgacgatg aattacctga attacgcttt cggctacgag
     1081 agcaatcatt tcgacgggct tctgaccagc cgcgttaaca tggatcttta catccgggcc
     1141 aagctcacga tcgggatgct gatcactacc ttttgtttca tcatcaccat tccgtacttt
     1201 ctgttttcga tggatatcct gcttatcaac ttcgtggcat tcctgtttaa cctgggtttc
     1261 ttatcatacc ttttattata tatggcaact ttcaataaga agaggatgga cctggcgaag
     1321 ggatcgagct tcaactacca gggcgtcggc ctgatgaact ggctggttct gattccggca
     1381 tttgtccttc cgttgatcat cttcgccccg ttcaacctta ccgggaacag gtacacgggc
     1441 cttgccgcca tcggtctgct gggcattctg ggtttgattt ccaggagatt ctgggtaaag
     1501 atgattgaaa agggatttta tgcaagaaaa tatacgatgg ctgaggggtt tagggagtaa
     1561 ggatgaatat caaccaataa atgctccgac gactaaagcc gtcggttgat gataatgtgc
     1621 tccgacgact aaagccgtcg gttgctgata atgtgctccg acgactaaag ccgtcggttc
     1681 tggggaggtt accttgaacc gatgggttta cccgtcggac tgcaatctta aaaaatacct
     1741 tcggactgca atcttaaaaa aatagtaatc atatgatcgg agtatccaat ctgtcaaaaa
     1801 tctactctgg cgtcaccgtc ctgaatatcg attcgctgga gatcaaagcc ggtgagagtt
     1861 tcggccttgt cgggaataac ggtgcgggta aaacaaccct tttccgcctg atcctcgacc
     1921 ttatccggcc tcagaccggt tttgtcactt ccaaagaaca ggatgtatcg cggtcggaga
     1981 actggaaact ttacaccggt tcttacctcg accggaattt cctgatcgat ttccttacgc
     2041 cggaagagta ttttgatttc aatgcccgta tccacgggat gtcgccggaa gatctgcaag
     2101 gtttcctgga atcgatgacg gagtttttta atgacgagat cctcgggaaa aagaaattca
     2161 tccgcgacct ttccaaagga aaccagcaaa agatcggcgt ggccgcagca ttcatggccg
     2221 atccggaagt catcatcctc gatgaacctt tcaacggcct tgatccgtca tcacagataa
     2281 ggctgatcaa tatactgaac aggttgaagg aaaacaaaag gacaacactg ctcatctcca
     2341 gccatgatct caaccacgtg accgaggtct gcagccggat cgccatcctg gaaaagggga
     2401 tcatcgtgaa agacctgcag accgaagaga acacccttaa agagcttcgg aattatttct
     2461 cggtctgagg aattgcctat ttttgcagca tgcgcaatat tttactgaaa tcactgctgc
     2521 ttatctcttt tttgctggca ttccagccgg atgccatgtc acagcaccct tactccgttt
     2581 ccggccgggt gattgacgcc gaaacccgcg aaccgctggc ttttgtcaat atcatccaga
     2641 ccggaaccaa aaccggtatt tccaccgata tcgacggcaa attcaccctg aaatccgacc
     2701 gcccgatcga tgcgatatgg ctgacgtatg tcggttattt cccgctacag tattccatcc
     2761 cctacgggga agtggataag attttgatta aattgcagcg taagactttc gatctcaatg
     2821 aagtcgttat tctgcccacg gctaatccgg cgcaccggat catcgacagc gtgctggcca
     2881 accgttaccg gaacgatccc gaaaaactgg catccttttc ttacacatct tatgaaaaaa
     2941 ccatcctgac agcaaaactg gatacgatga tgcgggtggg caaagacagc accggcaact
     3001 ggtactccga tacgctgatc atggagcccg acacggcagt gatgatgacc gacacgactc
     3061 tttctgacac ggcgctggcc gagatgaagg agtttttcga caaacaggat attgccatca
     3121 tggaatcggt ggtggaacgc aaattcttgg cacccgaccg caactatgag aatgttaagg
     3181 cgaaccgggt
//
LOCUS       DAOPNH010000333         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_37603_length_3131_cov_4.447334, whole genome shotgun sequence.
ACCESSION   DAOPNH010000333 DAOPNH010000000
VERSION     DAOPNH010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37603_length_3131_cov_4.447334"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKZ74_13555"
     CDS             <1..526
                     /locus_tag="PKZ74_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPM93827.1"
                     /translation="TWGEYLGHDDNSKINVYTRSDACGAAEMWGKFLGKNQESLLGVG
                     VFGDPGIADAVKNDALGLGFNNVIYAYDIKTRMKYPGMEVIPIDLNGNRVIDPDEDFY
                     GTLDLIVQAIQTGKYPSPPARDLYLVSKGKPDDMTVITFLKWILTDGQKYVHEGGYVD
                     LQEEKIRQELLKLE"
     gene            529..1431
                     /gene="pstC"
                     /locus_tag="PKZ74_13560"
     CDS             529..1431
                     /gene="pstC"
                     /locus_tag="PKZ74_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPM93828.1"
                     /translation="MAGLRIGRHLRSKLHLIWMMIGLVLVIFLPFFLGVGLYIKSELL
                     LRDQSVFDLILSGDWRPLSGKFGFFPFVISSIWITILSLLIAGPICLLTAIHLTQYAK
                     GWVLRIMHPVIDILAGIPSVIFGVWGILVIVPFISKYMAPLFGVSTSGYTIMAGAIVL
                     AVMIIPFVLNILIDVFKTIPDELKEASLSLGASQWQTIKLVLLRKAFPGIVSALGLGI
                     SRAFGETIAVLMVVGNVAKIPSGVFQPGYPLPALIANNYGEMLSIPMYDSALMLAALI
                     LFFVVLIFNFLSRLAIVRLEKQTS"
     gene            1431..2288
                     /locus_tag="PKZ74_13565"
     CDS             1431..2288
                     /locus_tag="PKZ74_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPM93829.1"
                     /translation="MKKLKYIEEKFFRVLMFVSTNLIVLVLLLIIFSILYKGLPSLSW
                     EMISQTPKGGFYFGKEGGILNAIIGSLYLSIGATLLAVIIGLPVALLMNVILVKRKKL
                     INSIRFLLDLLWGIPSIVYGAFGFTIMIYLGIKTSLLAGIITVTLFIIPIMVRAMDEV
                     LKTVHVGLLETSLSLGSTHSETAFKVFLRQASPGLVTAVLLSFGRAIGDAASVLFTTG
                     YTDHIPVSLGQPTATLPLSIFFQLSSPIEEVKNRAYASAVVLTVIILIISILARLYSK
                     RYQKNKINF"
     gene            2290..3051
                     /locus_tag="PKZ74_13570"
     CDS             2290..3051
                     /locus_tag="PKZ74_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPM93830.1"
                     /translation="MDQGVKILIKDLDLHIGKQHILKNINVAIPEKKITVILGPSGCG
                     KTTLMKSMNRLTDIYPEVTVTGQIFINDDDILHTTMDITKLRQKMGLLSQRPYPLPMN
                     IYNNIAYGLRISGRRKKKFLDKRVEHYLRVANLWDEVKDRLHDPASKLSIGQQQRLCL
                     ARGLAVDPEIILADEPTSALDPVSSQAIEEKFIELKKNYTIVLVTHILRQARRIADNV
                     IFMYMGEVVEAGTADQIFNDPKEEKTKSYFKGLFY"
BASE COUNT          801 a          727 c          738 g          865 t
ORIGIN      
        1 cacctggggc gagtatctcg gacatgatga caatagcaag atcaatgtct atacccggtc
       61 ggatgcgtgc ggagcagctg aaatgtgggg caagttcctg gggaagaacc aggaaagcct
      121 attaggcgtc ggagttttcg gagatccggg aattgcagat gccgttaaaa atgatgcact
      181 gggcctaggg tttaataatg tgatctatgc ctacgacatc aaaacgcgca tgaaatatcc
      241 gggtatggaa gtcatcccca ttgacctgaa tggcaacaga gtgatagatc ccgatgaaga
      301 tttttacggt acgctggacc tgatcgtaca agctatccag accggtaagt acccctctcc
      361 gcctgcccgc gacctgtatc ttgtatccaa agggaaaccg gatgacatga cggtcatcac
      421 cttcttaaag tggattttaa ccgatggcca gaaatatgtt catgaagggg gatatgtgga
      481 tcttcaggaa gaaaaaatca ggcaggaatt attaaaactt gaataattgt ggctggtctc
      541 agaatcggaa gacatctcag aagcaagctt caccttattt ggatgatgat cggcctggta
      601 ctggtcatct ttcttccttt ttttctggga gtgggattat atatcaaatc tgaactatta
      661 ttaagggacc agtcggtttt cgatctgatc ctgtcgggtg actggcggcc gctgtcggga
      721 aaattcggct ttttcccgtt tgtcatcagc tccatctgga taaccattct atctctttta
      781 attgccgggc ctatttgtct gctgacggcc atacatctca ctcagtacgc taaagggtgg
      841 gtactgcgaa tcatgcaccc ggttatcgat atcctcgcag gaataccttc ggtaatcttc
      901 ggcgtctggg gtatcctggt gattgtccca tttatctcga aatatatggc ccccctgttt
      961 ggcgtatcaa cctcgggata taccataatg gctggggcta tcgtgctggc ggtcatgatc
     1021 ataccttttg tactgaatat cctgatcgac gtattcaaaa ccattcccga tgagctgaaa
     1081 gaggcttccc tttcgctcgg agccagccaa tggcaaacca tcaagctggt actgcttaga
     1141 aaagcattcc cgggaatcgt atcggcgctc gggctgggta tttcacgcgc cttcggcgaa
     1201 acaatagctg tactgatggt ggtgggaaat gtggccaaaa tcccttccgg ggttttccag
     1261 cccggctatc ccctgccggc tttgatcgcc aataattacg gcgagatgct gtcgatcccc
     1321 atgtatgatt cagcgctgat gctggcggct ttgatcctgt ttttcgtggt gctgattttt
     1381 aactttttat cacggcttgc aatagtcagg ctggaaaaac aaaccagttg atgaagaaat
     1441 taaagtatat agaggaaaag tttttcaggg tgctgatgtt cgtctccacg aacctgatcg
     1501 tcctggtatt gcttctgatc atcttcagca ttctttataa aggccttcct tcgctctcct
     1561 gggaaatgat ctcgcaaaca cccaaaggag gcttttattt tggtaaggag ggcggaatac
     1621 taaatgctat aatcggatcg ctttacctgt caatcggcgc caccctgctg gcagtgatca
     1681 ttggcctgcc cgtcgccctg ctgatgaacg tcatcctggt caaacgaaaa aaactgatca
     1741 attccatccg gttcctgctc gatctgctct ggggcatccc gtcgattgtt tacggagctt
     1801 ttgggttcac aatcatgata tacctgggta tcaagacttc gcttctggcc ggtatcatta
     1861 cggttaccct atttataatt ccgatcatgg ttcgcgccat ggatgaagta ttgaaaacgg
     1921 ttcacgttgg tttgcttgag acttcattat ccctgggatc cacacattcg gaaacggctt
     1981 tcaaagtatt tctccggcag gccagccccg gactggttac agctgttctg ctctccttcg
     2041 gccgcgccat tggcgatgcc gcttcggttc tttttacaac gggctatacc gaccatatcc
     2101 cggtttctct cggccagccg actgccacgc tgccattgtc catctttttt caactttctt
     2161 caccaataga agaagtgaaa aacagggctt acgcttctgc cgttgttctg actgttatta
     2221 ttctgattat cagtattctg gccaggctct actcaaaaag gtaccagaaa aacaaaataa
     2281 atttctgaca tggaccaagg tgttaaaatc ctgataaaag atctcgacct gcatataggt
     2341 aaacagcata ttctgaagaa tatcaatgtg gcaatacctg agaagaaaat tactgtcatt
     2401 ctggggccat cgggttgtgg aaaaacgacc ctgatgaaga gcatgaaccg gttaaccgat
     2461 atatacccgg aagttaccgt gaccggccag atcttcatca atgatgatga tattcttcat
     2521 actacgatgg atatcacgaa actccggcaa aagatgggtc tgctttctca gcgcccctac
     2581 cctcttccga tgaacatcta taataatatc gcatatgggc tcaggatcag cgggagaaga
     2641 aaaaagaagt ttctcgataa acgggtggag cattatctcc gggtagccaa cctttgggat
     2701 gaggtgaaag acaggcttca tgaccccgcg tcgaaattat ccatcggtca gcaacagcgg
     2761 ctttgcctgg ccagaggact ggccgtggat ccggaaatca tcctggccga tgagcctact
     2821 tctgcccttg atcccgtttc cagccaggct attgaagaga aatttatcga actcaagaag
     2881 aattacacta tcgttttagt aacgcacatt ctcaggcaag ccaggcgcat tgccgataat
     2941 gtgattttca tgtatatggg tgaagttgtg gaggccggta cggcggacca aatcttcaat
     3001 gatccgaaag aagaaaagac aaagtcttat tttaaaggac tattttatta ataatcaaac
     3061 ctatacgtat tacgatgaaa aaaactgcat tgtttttctt attatttgca ttagccggat
     3121 caggagctta c
//
LOCUS       DAOPNH010000334         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_37852_length_3117_cov_7.428805, whole genome shotgun sequence.
ACCESSION   DAOPNH010000334 DAOPNH010000000
VERSION     DAOPNH010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37852_length_3117_cov_7.428805"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1685)
                     /locus_tag="PKZ74_13575"
     CDS             complement(123..1685)
                     /locus_tag="PKZ74_13575"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPM93831.1"
                     /translation="MHDRHKEIQQLLDKREQAKLGGGIQRIEAQHNKGKLTARERIDM
                     LLDEGSFEEYDMFMVNRGEDSGPRDQKILGDGVVTGHGTIDGRVVYVFSQDFTVFGGS
                     LSETFALKICKIMDQAMKVGAPLIGINDSGGARIQEGVRSLAGYAEIFERNILASGVI
                     PQISAIFGPCAGGAVYSPALTDFILMSDKTSYMFVTGPKVTKTVTGEDITEDDLGGAQ
                     VHSMKSGVSHFLAESEEEGIMLVRKLLSYMPQNNLEDPPVIECTDPIDRLDDHLNEII
                     PEDPNKPYDIKDVIYGIVDNTEFLEIQRHFAKNIVIGFARLNGFPVGIVANQPVFMAG
                     VLDINASRKAARFVRFCDAFNIPILTLVDVPGFLPGSAQEYGGIIIHGAKLMYAYGEA
                     TVPKVTVTLRKSYGGAHDVMSSKQLRGDINYAWPSAEIAVMGPAGAIEIIEGKTLKNM
                     ASEEERAEFIAIKEKEYRDKFANPYEAARYGYIDDVIEPRNTRFRVIRAFRTLATKKD
                     TNPLKKHGNIPL"
     gene            complement(1709..2119)
                     /gene="mce"
                     /locus_tag="PKZ74_13580"
     CDS             complement(1709..2119)
                     /gene="mce"
                     /locus_tag="PKZ74_13580"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPM93832.1"
                     /translation="MKISHIEHIGIAVKNLEEAIPFYENVLGLKCYAVEEVKDQKVKT
                     AFFKVGQTKIELLESTDPEGPIGKFIANRGEGVHHMAYAVENLEECLKEAEEKGIRLI
                     DQQPRKGAEGLHIAFLHPKSTAGVLTEFCEDKNH"
     gene            complement(2317..2553)
                     /gene="atpC"
                     /locus_tag="PKZ74_13585"
     CDS             complement(2317..2553)
                     /gene="atpC"
                     /locus_tag="PKZ74_13585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPM93833.1"
                     /translation="MKLEIITPEQTLFTGEATLVQMPGIDGSFEILSNHAPMISVLSA
                     GRIKIQDAQKQLQYFDVKGGVVEVLNNKVLVLAE"
     gene            complement(2555..>3117)
                     /locus_tag="PKZ74_13590"
     CDS             complement(2555..>3117)
                     /locus_tag="PKZ74_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPM93834.1"
                     /translation="QERITSTKRGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLN
                     RKIFEIGIYPAVDPLDSTSRILSPEIVGEDHYRTAQRVKQILQRYKELQDIIAILGMD
                     ELSDEDKLTVSRARRVQRFLSQPFHVAEQFTNIPGTIVPIEDTIKGFKMIMDGEVDEY
                     PEAAFNLVGTIEEAIEKGKKMLAENE"
BASE COUNT          680 a          806 c          791 g          840 t
ORIGIN      
        1 gccaaatact ctgtaaagga caaagagggc ggtaaaaacg atcacaatac cgactatgct
       61 gatgactata ccgtccttaa ctaaaatact tatttcctca ttcatattca ggtattttat
      121 ggtcagagag gaatgttacc gtgttttttc agcgggttgg tgtccttctt tgtcgccagg
      181 gtccggaagg cacggataac gcggaaccgt gtattccggg gttcgatgac atcatcgata
      241 tatccgtacc gggcagcttc gtatgggttg gcaaatttat cgcggtattc cttttcttta
      301 atggcgataa attcggcacg ctcttcttcc gaggccatat ttttcagggt tttcccttcg
      361 atgatctcga tggcgccggc agggcccata acggctatct ctgctgaagg ccaggcatag
      421 ttgatatctc cccgaagctg cttggagctc atcacatcgt gcgctcctcc gtaagatttc
      481 cgcagggtaa cggtcacttt ggggacggtg gcttcgccgt aagcatacat cagcttggcg
      541 ccgtggataa tgatcccgcc gtattcctgg gcgctgccgg gaagaaagcc gggaacatcc
      601 accagggtca ggatcgggat attgaaagca tcgcagaagc ggacaaagcg ggccgccttg
      661 cgggatgcgt taatgtcgag cacaccggcc atgaaaaccg gctggttggc gacgatcccc
      721 accgggaaac cgttgaggcg ggcaaaaccg atcacgatat tcttggcaaa atgccgctgg
      781 atctccagga attcggtgtt atccacaata ccgtaaatca catccttgat gtcgtaaggt
      841 ttattcgggt cttcgggaat gatctcgttc agatgatcat ccagcctgtc gatgggatcg
      901 gtacattcaa tgaccggcgg atcttcgagg ttattctggg gcatgtagct cagcagcttg
      961 cgcaccagca tgatcccctc ttcctcgctc tccgccagga aatgcgacac ccccgatttc
     1021 atggaatgga cctgcgcccc gccaaggtca tcttcggtaa tatcttcgcc ggtaaccgtc
     1081 ttggtgactt ttggcccggt gacgaacatg tagctggttt tatcgctcat gaggatgaaa
     1141 tcggtgaggg ctggcgaata gacggccccc cctgcacatg gaccgaaaat ggccgatatc
     1201 tgcgggatca cgccggaagc caggatgttc cgctcgaaaa tctcggcata gccggccagg
     1261 ctcctgacgc cttcctggat acgggcgccg ccgctgtcgt tgataccgat caaaggcgca
     1321 ccgactttca tggcctggtc catgatcttg caaatcttca gggcaaaggt ttccgataag
     1381 gaaccgccga atactgtgaa atcctgcgaa aacacataca ccacccgccc gtcgatcgtc
     1441 ccgtgtcccg ttaccacacc gtcacccagg attttctggt ctctcggccc cgaatcttct
     1501 ccccggttga ccataaacat gtcgtactcc tcgaagcttc cttcatcaag cagcatgtcg
     1561 atcctttccc gggctgtaag ttttcctttg ttatgctgcg cttcaatcct ctggattccg
     1621 ccgcctaatt tagcctgttc ccgcttgtca agaagttgct ggatctcctt gtggcggtca
     1681 tggataaatc tctcttttgc catatttact aatgattttt atcttcacag aattcggtca
     1741 gtaccccggc tgtcgatttt ggatgcagga acgcgatatg cagtccttcg gctcctttgc
     1801 gcggttgctg gtcgatcagg cggatcccct tttcttcggc ttctttcagg cactcttcca
     1861 ggttttcaac cgcataagcc atatggtgaa ccccttcgcc ccggttggca atgaacttcc
     1921 cgatcggccc ttcgggatcg gtcgattcca gtaattcgat cttggtctgc ccgactttga
     1981 agaacgccgt cttcactttc tggtctttca cctcttctac agcataacac ttgagtccga
     2041 gcacgttctc ataaaatggg atcgcttctt caagattttt gacagcaatt ccaatatgct
     2101 caatatgaga tattttcatg atagaaaaag attattgtta attttttcac aaaggtagga
     2161 aatattttaa gagatggcat ctcagaaatg gttatttttt tctgaaatgc tttgataatc
     2221 ttgcattccg gaagattcag atgaaatagt gagatgccat ctcaaagagt tataaaaaag
     2281 atttataact acctgtagtt ctacgggtta caacaactat tcggctaaaa ccagcacttt
     2341 gttgttcagc acctctacca ctccgccttt tacgtcgaaa tactgaagct gtttctgggc
     2401 gtcctggatc ttgattcggc cggccgacag cacggagatc atcggtgcat ggttgctcag
     2461 gatctcgaaa gagccgtcga tgcccggcat ctgaaccagg gtggcttcac cggtgaatag
     2521 cgtctgttcg ggggttatga tttccagttt catatcattc gttctctgcc agcattttct
     2581 tccctttctc gatagcctct tcgatggtgc ctacgagatt gaaagctgcc tccgggtatt
     2641 cgtcgacctc gccatccatg atcatcttga atcctttgat ggtatcttcg atcgggacga
     2701 tggtgccggg aatattggta aactgttctg ctacatggaa cggctgcgac aagaagcgct
     2761 ggacacggcg ggcacggctc accgtgagct tgtcttcatc cgagagctcg tccattccga
     2821 ggatggcgat gatatcctgc aattccttat agcgctgcag aatctgcttc acgcgctgtg
     2881 cggtccggta atggtcttcg ccgacgattt cagggctgag gatgcgggat gttgaatcca
     2941 gcgggtcgac agccgggtag ataccgatct caaaaatctt acggttgagt acggtagtgg
     3001 catccaggtg ggcaaaagtc gtggccggtg cgggatcggt aaggtcgtcg gctggtacgt
     3061 agaccgcctg tacggaggtg atggaaccgc gcttggtgga ggtgatgcgt tcctgca
//
LOCUS       DAOPNH010000335         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_37860_length_3117_cov_4.455911, whole genome shotgun sequence.
ACCESSION   DAOPNH010000335 DAOPNH010000000
VERSION     DAOPNH010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37860_length_3117_cov_4.455911"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PKZ74_13595"
     CDS             <1..1064
                     /locus_tag="PKZ74_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM93835.1"
                     /translation="RHTDIQIAIERTQKFLQLNRKLKNMEERHSLISKTLEDKIDRHF
                     IGKSPQIKEVFEKAVTAARYPDANVLITGESGTGKENIARIIHYSSDRKDHTFFAVNS
                     SAITDSLLESEFFGHKKGSFTGAINDKMGYFEVCHEGTLFLDEIADMPLNLQAKLLRA
                     AEEKVITRIGDTKPIQTNFRIISATNHDINEMVREKKFRLDLLHRLNTVHIHIPPLRE
                     RREDIKPLLMHFAQEYAVRFNKPDFKITKEVIDALMKYDFPGNVRELKNMTERAFILC
                     RGNEFTCYDFPVSTQPMIAENTSGTFNMKNLEQELVRDALKHCNYNQKAAANMLGITR
                     DALIRKMKKYNITINKSDT"
     gene            1301..1987
                     /locus_tag="PKZ74_13600"
     CDS             1301..1987
                     /locus_tag="PKZ74_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM93836.1"
                     /translation="MNATTTTLNFTESESILTPLLNLVDRRQTSTRGHKIVYTNEAII
                     YGLKARDEKVISYIYKQSYAQVRFFITSNNGTVMDAEDIFQDALVLIYKNIASEKFVL
                     TCSFSTYLFSICRHLWLQKLNRNLRNVSIDDNMTSCDPFEDEEKLKDHLMESEKYRLF
                     QKHFNRLKAGEQKILKLYMSKTHGREVASIMGYKSDKYAKYRKYVIKEKLKAMIMSDE
                     LYDKVCSLNN"
     gene            2429..>3117
                     /locus_tag="PKZ74_13605"
     CDS             2429..>3117
                     /locus_tag="PKZ74_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93837.1"
                     /translation="MSLLLLLPGSLSGQTEPVYPSLIVTGEYLGLTPPLRDLPAISEM
                     ETMKMRNDAAGKILNSRLRTRSYPYADQAFPKGADPVWQKASGTRNSDRAPDLNISGQ
                     DSPFYPSDPTGAAGPGHYMQAVNAAYTIYDKNGVLLAGPTALNTLFEGVAGSECNSGD
                     PIVLYDEQAGRWLVAEFSICGANDFVLIAISTTSDPTGAWHKYSFDVDDIPDYEKFGI
                     WRDGYYMGTNNA"
BASE COUNT          881 a          796 c          679 g          761 t
ORIGIN      
        1 tccggcatac cgacatccag atcgccatcg aacgcaccca gaagtttctt cagctcaacc
       61 ggaaactgaa aaacatggag gagcgccatt cgctcatctc caaaacactt gaagacaaaa
      121 tcgaccgcca tttcatcggg aaaagccccc agataaagga ggtattcgaa aaagccgtta
      181 cggctgcccg ttaccccgat gccaatgtac tgatcaccgg cgaaagcggc accgggaagg
      241 aaaatatcgc ccggatcatc cactattcca gcgaccggaa agaccatact ttttttgccg
      301 tcaacagcag cgccatcacc gactcactgc tggaaagcga attcttcggc cataaaaagg
      361 gctcctttac cggagccatc aacgataaaa tgggctactt tgaagtgtgc cacgaaggga
      421 cgcttttcct cgatgaaatt gccgacatgc cgctgaacct gcaggccaaa ctcctgcggg
      481 ccgcagaaga gaaagtcatt acccgtatcg gcgataccaa accgatccag accaatttcc
      541 ggatcatctc ggcgacaaac cacgacatca acgagatggt cagggagaaa aaattccggc
      601 tcgacctgct ccaccggctc aataccgttc atatccacat cccgccgctg agggaaagaa
      661 gggaagacat caaacccctg ctgatgcact ttgcccagga atacgcggtg aggttcaata
      721 aacccgattt taaaatcacc aaagaggtga tcgatgccct gatgaagtac gacttccccg
      781 gtaatgtcag agagctgaaa aacatgaccg aacgggcctt catcctttgc cgggggaatg
      841 agttcacctg ctatgatttc cccgtaagta cccagccaat gatcgctgaa aacacatccg
      901 gaactttcaa catgaagaac ctggagcagg aattggtcag agacgcgctg aagcactgca
      961 attacaacca gaaggcagcc gccaacatgc ttggcatcac ccgcgatgcc ctcatccgta
     1021 aaatgaaaaa atacaatatc acgatcaata aatcggatac ctgaccaaac cggattgcgc
     1081 acacctgtgc gattattttt tcgggcttcc tattattctc ttattatatt cataatattc
     1141 atatgtctga atgttttttt atgattcgta atcctctcat tatgtttaaa ttaaaaaata
     1201 ttttatcaaa aaaatgaatt gtccggcttc ttaggtatgg ggtttgcaaa tgggcggcca
     1261 gtgtttaact caaaatacaa acaacaaaga tcccatccca atgaacgcaa cgactaccac
     1321 cctgaacttt acagaatccg aaagcattct gactccgctt cttaatttgg ttgaccgcag
     1381 gcaaacatca acccgggggc ataaaatcgt ttataccaat gaagccatca tttatggcct
     1441 gaaggcccgg gatgaaaagg tgatcagtta tatctacaag caatcgtatg cacaggtgag
     1501 gttttttatc acctccaaca acggcacggt gatggatgca gaagatattt tccaggacgc
     1561 gctggtgctc atctataaaa acatcgcctc agaaaaattc gtattgacct gttccttcag
     1621 cacttacctg ttttcgatct gcaggcacct ctggcttcaa aaactgaaca ggaacctgag
     1681 aaatgtaagc attgatgata acatgaccag ttgtgatcct tttgaagatg aagaaaagct
     1741 gaaagatcat ttgatggaat cggagaaata ccgcctgttt caaaagcact tcaacaggct
     1801 caaagccggg gagcaaaaaa tactgaaact ctatatgagc aagacccacg ggcgggaggt
     1861 ggcgtcgatc atgggttaca agtcggataa atacgcaaaa taccgtaaat acgtcattaa
     1921 agaaaaactg aaggctatga tcatgagtga tgaactttat gataaagtct gcagccttaa
     1981 taattaataa ttagtagtgc ataatattta aaaaactctt ttcataattt ttttaagtat
     2041 ttgttttcaa ttcacgtggc tgttcattca tttgaacagc ctttttttat taccgacgag
     2101 tgtcccgtcg gttcttagaa agattctctt ccgacgactg aagccgtcgg ttctattttt
     2161 tttggaacca acggctttag tcgttggagc acgatcacca tcaaccgacg gatttacccg
     2221 tcggagcacc aaacattcac cgacgactaa acccgtcgga gtatcgaaaa aaaccggtta
     2281 gacggacttt tgagatggcc tctttcttaa tttcgacaat ctatgtcgaa aaaggcaaaa
     2341 tttatattat cttcgtaacc cttttccatt tttgccagta aacaagctaa ccagaccttg
     2401 tcgaatgaag gctgttattt ttttgccctt gtctttactg ctcctgctgc cgggatccct
     2461 ttccggacag acggaacccg tttatccttc tttgatcgtg accggcgagt acctgggtct
     2521 gactccgccg cttcgtgatc tgccggccat ttcggaaatg gagacaatga aaatgaggaa
     2581 tgatgccgcc gggaagatcc tcaacagccg tttgcgcacc cggtcgtatc cgtatgccga
     2641 tcaggctttc ccgaaaggtg ctgatccggt atggcaaaaa gcctccggta cgcggaattc
     2701 ggatcgtgcc cccgacctca atatctccgg ccaggattcg cccttttatc catccgatcc
     2761 caccggggcg gcaggtcccg gccattacat gcaagctgtt aatgccgcct atactattta
     2821 tgacaagaac ggcgttctgc tggccggtcc gaccgccctc aacacccttt tcgaaggggt
     2881 cgcgggttcg gaatgcaaca gtggcgaccc gatcgtcctt tacgacgaac aggccggacg
     2941 gtggctcgtc gccgagtttt ccatctgcgg tgccaacgac tttgtgctta tcgccatttc
     3001 caccaccagt gatccgaccg gcgcctggca caaatattcg ttcgatgtgg acgatatccc
     3061 cgattacgaa aaatttggca tctggcgcga cggatattac atgggcacca ataatgc
//
LOCUS       DAOPNH010000336         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_37967_length_3111_cov_4.947644, whole genome shotgun sequence.
ACCESSION   DAOPNH010000336 DAOPNH010000000
VERSION     DAOPNH010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37967_length_3111_cov_4.947644"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKZ74_13610"
     CDS             <1..561
                     /locus_tag="PKZ74_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93838.1"
                     /translation="LRHIMPNLTLVILTCPEAPETVFQDFGIGQLINGYAHIPIDPDL
                     VININVSDQHPLKVYVTPEGDCNGLYVTNKSAEGFDVFELQGGKSNISFSWQIVATRA
                     DEQYMLTNGKTEVAVNSQRFPPAPGPLESEGFDLRPVKLTDMEILKIETEKYKTKNLV
                     EDKLIESQTLEPVEDHVIEIIDNPHE"
     gene            554..2104
                     /locus_tag="PKZ74_13615"
     CDS             554..2104
                     /locus_tag="PKZ74_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93839.1"
                     /translation="MNKLLRHTIHGLRIMPALVFSILSHLAAAQVSISPGTWITVKEG
                     GSLYIGTDLHIKSTADSSGYFVDQTIDGDVTITGDITVDRYMAPDIWHNVASPVSNET
                     SACFTGTDLVYWYDETQIWNDWNFGWVWYTGATGGPLTVFRGYDVFFPDNPVTVNYSA
                     TGAETLNTGNYIYNVTVTDPTPNPIEIPSHVGWNLAGNPYPSPVDWLAASGWDKSDIN
                     DAKYIWDGDNDIYTIFIGGGSPIGINGGTRYIPSNQGFWVQAVQNGTLGISNAVRLGN
                     MTGTPDFYKNETPGYPLISLIAESNGRSDEAVVRFIEGTTAGFDKNFDASKLFSPSDE
                     VPQLSIRAGKQSLALNTFPNPFLSNPIELDFQCGEKGIFTIRLGERTWIPDTLKVYLK
                     DELENRMTLLNSDAPYNYFHHPNNSRDRFKLYINPPAAITENLPGVSNFIVYSSGNRV
                     TIIKNTSLVLSGEIDIINLLGQVVEKAHYNNEETRNLTLNLPGGEYFIMIKTKSNIFR
                     SNFFINNK"
     gene            2201..3016
                     /locus_tag="PKZ74_13620"
     CDS             2201..3016
                     /locus_tag="PKZ74_13620"
                     /inference="COORDINATES: protein motif:HMM:NF017810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fn3-like domain-containing protein"
                     /protein_id="HPM93840.1"
                     /translation="MKRTRYPLLLLVSAAICLLGMDTYAQELEVSPGTLMFATNPGSS
                     QTQQITLRNKGKTEQSFIFNLSDWLTDENGEVKYFDPGTTTRSCTDWITVSPALVTLQ
                     PNEQATINVTMLVPNDNPSTKWAVLFVQSAVEQTGPRAVDKEMALGVQLALRIAVTIY
                     QSPASNTLYKATIEGLTEKIGEDNSRTYGSQVINLGDKVLNCKVYFTISNLETAEEFT
                     SAPFEFSLLPETSKKVSYKHDKPLEKGKYSVAAILDYGFNNELDGVQLEIDVK"
BASE COUNT          911 a          752 c          672 g          776 t
ORIGIN      
        1 ttacgtcata ttatgcctaa cttaacacta gtaattttaa cctgtcccga agctccggaa
       61 actgtctttc aagattttgg aataggacag cttattaatg gttatgcaca tatcccgatc
      121 gatcctgatt tagtgatcaa tataaatgta tctgatcaac atccactaaa agtgtatgta
      181 acacctgaag gggattgtaa cggactctat gtgacaaata aatctgctga aggttttgac
      241 gtgtttgaac ttcagggtgg gaaatctaat atttctttct catggcaaat tgttgcaaca
      301 agagctgatg aacaatacat gctcactaac ggcaaaacag aagttgctgt taatagtcaa
      361 aggtttccac ctgctccagg cccattggag tctgaaggat ttgatcttcg gcctgtaaaa
      421 ctgacagaca tggaaatact aaaaatcgaa acagaaaaat ataaaactaa gaatcttgtc
      481 gaagacaaat taattgaatc ccagacttta gaacctgttg aagatcatgt gattgaaata
      541 atagacaatc cgcatgaata aactcctccg gcatactatt catggcttga gaataatgcc
      601 agcactggta ttttcaattc tatctcatct ggcagccgcc caggtatcca tcagcccagg
      661 cacatggata acggtgaaag agggcggttc gctctacatc ggaaccgacc tgcatatcaa
      721 atccacagcc gattcttccg gctatttcgt cgatcagacc atcgatggcg atgtcaccat
      781 taccggggat ataaccgtag accggtatat ggctcccgat atctggcata atgtggcgtc
      841 gcccgtcagc aatgaaacca gcgcctgctt taccggtacc gacctggttt actggtacga
      901 tgagacccag atctggaacg actggaactt tggctgggta tggtataccg gcgcgaccgg
      961 aggcccgctc acggtattcc ggggttatga tgttttcttc ccggataatc cagtaacggt
     1021 caactattcc gccaccggag ccgaaaccct gaataccggc aactatattt acaatgtgac
     1081 cgtcaccgat ccgacaccca acccgatcga gatcccttcg catgtcggat ggaacctggc
     1141 cgggaacccc tacccttcgc cggtcgactg gctggcggcc agcgggtggg acaagtcaga
     1201 catcaacgac gccaaatata tctgggatgg ggataatgac atctacacga tttttatcgg
     1261 aggcggatcc ccgatcggga tcaacggcgg aacacgatac attccgtcaa accagggatt
     1321 ctgggtccag gcggtacaaa acggtactct gggcatcagc aatgcggtca ggcttggaaa
     1381 tatgaccggc acccccgact tttacaaaaa cgaaacgccc ggctatccgc tgatcagcct
     1441 tatcgccgaa agtaatggcc gcagcgatga agccgtcgtt cggttcatcg aagggacgac
     1501 cgccgggttt gacaaaaatt ttgacgcatc caagcttttc agcccttcag atgaggttcc
     1561 gcaactgagt atccgggccg gaaaacagtc ccttgccctg aacacctttc cgaatccctt
     1621 ccttagtaat cctattgagc tggattttca gtgcggggaa aaagggatct ttacaatccg
     1681 gctgggagaa aggacctgga ttcccgatac actgaaagtt tacctcaaag acgaactgga
     1741 gaaccgtatg acactgctga acagtgatgc tccatacaat tattttcatc atcccaataa
     1801 ctccagggat agatttaagc tttacatcaa ccctcctgca gcgatcaccg aaaatctccc
     1861 gggtgtcagt aactttatcg tatattccag cggaaaccgg gtaaccatta taaaaaacac
     1921 ttccctggtg ctatcggggg aaattgatat tataaaccta ctcggacaag tagttgaaaa
     1981 ggctcactat aataatgaag aaaccaggaa tttgacacta aatttgccag ggggtgaata
     2041 tttcatcatg ataaaaacaa aaagtaatat atttagatcg aattttttta ttaacaacaa
     2101 ataaaggaac catctgaata actacaggtt aaaaatattc ctgttgatat ttaagattat
     2161 caatcgaact attattaatt atctttgaaa aaaaaatctt atgaaaagaa ctaggtaccc
     2221 tcttttacta ttggtttctg cagcgatctg cttacttgga atggatactt atgctcagga
     2281 actggaagtc tctcccggca cactgatgtt tgccaccaat cccggctcct cccagactca
     2341 gcaaattacc ctgagaaaca aaggaaagac ggaacaatct ttcatattta atcttagcga
     2401 ctggttgacg gatgaaaacg gtgaagtgaa atatttcgat cccggaacaa caacacgatc
     2461 ctgtaccgac tggatcaccg tatctcccgc attggttacg ctgcaaccca atgagcaggc
     2521 tacgatcaat gtgaccatgc tggttccgaa tgataaccct tccactaaat gggcggttct
     2581 tttcgtacaa tcggctgttg aacagaccgg tccccgggct gtcgacaaag aaatggcact
     2641 gggtgtgcag cttgccctcc ggattgctgt gaccatctac cagtcgcctg ccagcaatac
     2701 actgtataaa gccaccattg aagggcttac ggaaaagatc ggcgaagaca actcccgcac
     2761 ttacggttca caggtgatta acctgggaga taaagtattg aactgcaagg tgtacttcac
     2821 catttccaac cttgaaacgg ctgaagaatt cacttccgcc ccattcgagt tttccctgct
     2881 accggaaacc agcaagaaag tttcctataa gcacgacaaa ccactggaaa aaggcaaata
     2941 ctcagtagcg gccatccttg actacggatt caacaacgag ctggatggcg tgcaacttga
     3001 aatagacgtc aagtagcgcc tgcaatgaat aaacggtgtc tgctcttcct tctgttcctc
     3061 catccggttt tcattatctc ctacgcacag gacgtgcagt atattgatac t
//
LOCUS       DAOPNH010000337         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_38476_length_3082_cov_7.000000, whole genome shotgun sequence.
ACCESSION   DAOPNH010000337 DAOPNH010000000
VERSION     DAOPNH010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38476_length_3082_cov_7.000000"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKZ74_13625"
     CDS             <1..320
                     /locus_tag="PKZ74_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPM93841.1"
                     /translation="QPLLHGEAIAAGMICETYLSFVLCGLGRKVLEDLTGFIAGHFRL
                     PALNGDATRDMNRIMAHDKKNRSDEIRFTLLRDAGNPLTGVSAGNSLVNEAFLYYSEI
                     AAK"
     gene            317..1570
                     /locus_tag="PKZ74_13630"
     CDS             317..1570
                     /locus_tag="PKZ74_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPM93842.1"
                     /translation="MISISVNSTTLNGTVILPASKSISNRLLILQSRYGNRLKIENLS
                     DSDDTLLLASTLDIVNRYMLTEENGLLRIDARNAGTVLRFLVPLLSITPGHFLLTGIE
                     RMKNRPVGALVDAMRELGASIEYAENTGFPPLLIKGRRIYGHRISVDSSVSSQFVTAL
                     LLLAPTLPDGLTIDLKGTSVSSPYVRMTTGILGKLGIEVISQQDSIRVFPKPELNASF
                     KVEADWSAASFWYCLMSMAESGRVVLPGLKKSNMQGDQSLIDIFRYLGVRTLEENDEI
                     IIERYGTPAEFFAADFTDTPDLALPVIMACAVAGTDGRFTGLESLRLKESDRIEALGA
                     GLAKTGVILFEEYPGAWRIKGRMAARGQLTPEDFSDHRVAMTFACLAMKGYRVHLNNP
                     SVVNKSYPGFWKDLQSVGFNCKVSN"
     gene            1641..2363
                     /locus_tag="PKZ74_13635"
     CDS             1641..2363
                     /locus_tag="PKZ74_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM93843.1"
                     /translation="MVTVDIFIPCFIDQIYPQTGFNMIKILEKYDIDVYYNEEQTCCG
                     QISFNSGFWDDAKAIGEKFILEFNHNRPVVVPSASCAGFVRNYYEQLFLNTGLHLEFR
                     QLQKNIVEFTDYLVNTLKVSDLGAVFEHKVTYHDSCAALREYKLGNEPRTLLSHVRGM
                     ELVEMKDTDVCCGFGGTFSVKNEPISTAMAQQKVQNALDTGAEYIVSTDASCLIHLDG
                     YIRKHNIPLKTIHIIDVLASGW"
     gene            2454..>3082
                     /locus_tag="PKZ74_13640"
     CDS             2454..>3082
                     /locus_tag="PKZ74_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93844.1"
                     /translation="MITSTKKAPGIFPVILMFSLLTAHCSLVFSQVPTVQDCMGAIPV
                     CQDVYVEENSYSGDGNYNNEIYEDLPCNTACPGSCLAGEVNSVWYIWTVQVGGLLRLT
                     IDPVQSTDDYDWAVYDVTDLRCDDIYTSYALMQKSCNAYGAGSNGNTGISTQNGGNSN
                     CNNCGATNIWNKDLPVVEGRTYVLIIENWSGTTQWHTSKHSVPTRGPPR"
BASE COUNT          785 a          725 c          768 g          804 t
ORIGIN      
        1 cacagccact tttacacggt gaagcgattg ctgcgggcat gatctgcgaa acctatcttt
       61 cttttgtgct ctgcgggctc ggccgaaaag tccttgaaga tttgaccggt tttatcgccg
      121 ggcatttcag actgcccgcg cttaacgggg atgccacccg tgatatgaac cggatcatgg
      181 cgcacgacaa gaagaatcgt tcggatgaaa tccggtttac actgctccgt gatgcgggga
      241 accctctaac aggcgtcagt gccggaaata gccttgtaaa cgaggcattt ttatattata
      301 gtgaaatagc ggcgaaatga tatccatctc tgtaaatagc acgacactga atggtacggt
      361 catcttaccg gcctcgaaaa gcatttcgaa tcggttgctg atccttcagt cgcgctatgg
      421 taaccggctt aaaatcgaga atctgtcgga ttcggatgat accctgttac ttgcatccac
      481 gctggatatc gtcaacaggt atatgttgac ggaagagaac gggctgctga gaatcgatgc
      541 gcgaaatgcc gggaccgttc ttcgttttct tgttccgttg ctctcgataa cacccggcca
      601 ttttctgctc accggcatcg aaaggatgaa aaatcgtccg gttggtgcat tggtagatgc
      661 catgcgggaa ctgggagcct ccattgaata cgctgagaat accggatttc cgccgctgct
      721 gatcaagggc aggcgtattt acgggcaccg gatttcggtt gacagttcgg taagcagcca
      781 gtttgttaca gcgcttttgt tactggcccc aacacttcca gatggactga ctatcgacct
      841 gaaggggaca tcggtatcat cgccttatgt tcggatgacg accgggattc ttggcaaatt
      901 gggcatcgag gtaatcagcc agcaggattc catccgtgtt tttccgaaac cggaactgaa
      961 cgcttcattt aaggttgaag ccgactggag cgcagcatcg ttctggtatt gcctgatgtc
     1021 gatggccgaa agcggacggg tagttttacc gggactcaaa aaatcaaata tgcaggggga
     1081 ccagtcgctg atcgatattt tcaggtatct gggggtaagg actttggagg agaatgatga
     1141 aataatcata gagagatacg gaactccggc ggaattcttt gctgccgatt ttaccgatac
     1201 gcccgatctc gctttgccgg tgatcatggc ttgtgcggtg gccggaaccg atggccgttt
     1261 taccgggctg gagagcctcc ggctgaaaga atcagaccgg atcgaagcgc tgggagccgg
     1321 gctggctaaa accggggtca ttctttttga agaatacccg ggcgcctggc ggattaaagg
     1381 aagaatggca gccaggggcc agctgacccc tgaagatttc agcgatcacc gggtggccat
     1441 gactttcgcc tgcctggcca tgaaaggtta ccgggttcat ttgaacaacc cgtcggtcgt
     1501 gaataaatcc tatcccggat tctggaaaga ccttcaatct gtaggtttta actgcaaagt
     1561 ttcaaattga tgaggaattg aatccgcctt aagataattt gatacctttg cccctgattc
     1621 gtcatttttt catcagaatt atggtaactg tcgatatttt catcccgtgc ttcatcgatc
     1681 agatctatcc ccaaacgggc ttcaatatga tcaaaatact ggagaaatac gatatcgatg
     1741 tttactataa tgaagaacaa acctgctgcg gacagatctc tttcaacagc ggattctggg
     1801 atgacgcaaa agccatcggc gaaaagttta tcctggagtt caaccacaac aggccggtcg
     1861 ttgttccgtc tgcttcctgt gcaggttttg tcagaaatta ttacgaacaa ttgttcctga
     1921 atacaggttt acatctcgaa ttcaggcagt tgcagaaaaa tatcgtcgaa tttaccgatt
     1981 acctggtcaa tactcttaaa gtttccgacc tgggcgccgt atttgagcat aaagtcacct
     2041 atcatgactc ctgcgccgcc ctgcgggaat ataaactggg aaatgagccc cgaaccctcc
     2101 tcagccatgt cagaggcatg gaactggtcg aaatgaaaga tacagatgtg tgctgcggat
     2161 ttgggggcac tttttcggtc aaaaacgaac ccatatcaac ggccatggcc cagcaaaaag
     2221 tccagaacgc cctggatacc ggtgcagagt atattgtatc gaccgatgct tcctgcctga
     2281 tccatctcga cgggtacatc cgtaaacata atattccttt gaaaaccatt catattattg
     2341 atgttctggc ttcaggatgg taaaaaatca cccgcagcat tcaaatttta tatccgtttt
     2401 tattcgattt taactatttt tggagtggca tcagcttcat taacctattg cttatgatta
     2461 cttcaacgaa aaaagcgcca ggcatatttc ctgttatttt aatgttttca ctgctcaccg
     2521 ctcactgttc acttgtcttt tctcaggttc ctacggttca ggactgtatg ggtgccatcc
     2581 cggtttgcca ggatgtatat gtcgaagaaa attcatacag tggcgacggc aattataaca
     2641 atgaaattta tgaagacctg ccgtgcaata ccgcttgccc gggcagttgc ctggctggtg
     2701 aggtgaacag tgtctggtat atctggaccg tccaggtcgg aggccttttg aggctcacga
     2761 tcgaccctgt tcagagtacc gatgattatg actgggctgt ttatgatgta accgacctgc
     2821 gttgcgacga tatttatact tcatacgccc tgatgcaaaa aagctgcaac gcttatggcg
     2881 ccggttcaaa tggcaacacc gggatcagca cgcaaaacgg aggaaacagc aattgcaata
     2941 attgcggagc tactaatatc tggaacaaag accttcccgt cgtcgaagga cggacctatg
     3001 tgttgatcat cgaaaactgg agcggaacca ctcaatggca tacaagtaag cattcagtcc
     3061 cgacacgcgg accgcccagg tg
//
LOCUS       DAOPNH010000338         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_38555_length_3078_cov_5.957658, whole genome shotgun sequence.
ACCESSION   DAOPNH010000338 DAOPNH010000000
VERSION     DAOPNH010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38555_length_3078_cov_5.957658"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKZ74_13645"
     CDS             <1..775
                     /locus_tag="PKZ74_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93845.1"
                     /translation="FTAFTGVSAPFALAPDYREQLQVSEYPYEEADLLLAAVEPTDPA
                     LVDELIQLPALSDNEKSLLQYNYAYRNADFAVAGTHLASYSPTDEEGTDFKILALYDL
                     DIIMYGRDILTGGDIEIIRAIAEKLSVNSNFAITLLNNSPEYRDHLFEPVPLPEVLAG
                     ADIKRIGEEAMHLVIRPNPASDKVFIESVFSPLSEVRVEMHDVSGKLITGYSLQRAAG
                     GIELDISRLREGFYFVVLTDPATGTVKTGKLIKVKSPGN"
     gene            821..2698
                     /locus_tag="PKZ74_13650"
     CDS             821..2698
                     /locus_tag="PKZ74_13650"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPM93846.1"
                     /translation="MVFLLAGWAGGQSASPLQGGETIANAFVITGTLPFSASGTTLGY
                     TDDYDEACPYDSSQAPDVVYSYTPAASVIVDIDLCGSEYDTKIFVYENTVTPGIPYDC
                     NDDFYYEEPCGLYVSKIEGLLLTGGNTYYIIVDGYSSGDFGNYDLSVTQSDNTPQCII
                     GINFICPAWAVDEPEDCGQDENGGCNMIPGNESWESVPSAGATYCGSLFADNGDKDSD
                     WYELVLSEPSSVVLTANAEQQISYGLAETTTPGAPTCITLTGDTEPGAVAGPCSETSV
                     DLGTLAPGTYWLVIEMTATSGFPCSNHYVFDIDVNPVPCPPPDFLTATGITSFGATLG
                     WFETGTAASWEYQYGVSGFTPAGSGISTVANPVAVTGLAISTAYDFYARAVCGPGIYS
                     AWSGPYTFTTLCGTINSIPWSEGFESGWPPACWTDDNALPGYGWDHGVYGSPGSGSKW
                     AYSNLSGSVLTSPSISLSADAYLFFSYRAENKDFPRNISVKINNDEVFQVNGFTSEDY
                     SVGAVSLATYIGQTVAIAFETTAGSGGQDFGICLDDVTVKFAVKWTGNLGTAWSNPGN
                     WSTSSIPGINDVVVVPSVPSGGNFPEVGSGITATCYFLELAPGATLKVKTGGTLNVLN
                     Y"
BASE COUNT          715 a          830 c          771 g          762 t
ORIGIN      
        1 attcactgcc ttcaccgggg tcagcgcacc gttcgccctg gcccccgact acagggaaca
       61 gctccaggtt tctgaatatc cttatgaaga ggccgacctt ctcctggctg ccgttgaacc
      121 cacggatccc gctttggtcg atgagttgat tcagttgccc gctctgagcg acaatgaaaa
      181 atcgctcctg cagtacaatt atgcttaccg gaatgcggac tttgcagtgg ccggaacgca
      241 cctggcaagc tattctccca cggatgaaga aggcaccgat ttcaagatcc ttgccctcta
      301 cgacctggat atcatcatgt atggccgcga tatactcacc ggcggcgaca ttgagatcat
      361 ccgggcgatc gccgaaaaac tgtcggtcaa ttccaacttc gccatcaccc tgctgaacaa
      421 ttcgcccgaa taccgggatc atctctttga acctgttccg ttgccggagg tattggccgg
      481 agctgatatc aaacggatcg gggaggaggc catgcacctg gtcatccggc ccaacccggc
      541 atccgataag gtgtttatcg aatcggtctt cagcccgctg tccgaggtcc gggtcgaaat
      601 gcacgacgtg agcggcaagc tcatcaccgg ctattccctg caaagggcag ccggaggtat
      661 cgagctggat atcagccgcc tcagggaagg gttctatttc gtggtcctga ccgatccggc
      721 gaccggcacg gtgaaaaccg ggaagctgat caaagtgaaa tctcccggta attaatcctt
      781 agcattcggt cgtttgaaaa caacatcgct aatatatgtt ttggtttttc tccttgcggg
      841 atgggcaggc gggcaatccg cttcccctct ccagggaggc gaaaccatag ccaatgcatt
      901 tgttataacc ggaaccctgc ccttctcggc cagtggtact acgctgggtt atactgacga
      961 ttatgatgaa gcctgccctt acgacagcag ccaggcaccc gatgtggtct attcctacac
     1021 cccggctgct tctgttatcg tggatattga tctctgcgga tcggaatatg acacgaagat
     1081 ctttgtctat gaaaataccg taactcccgg tattccatat gattgtaatg atgatttcta
     1141 ttatgaagaa ccctgcgggc tttatgtctc aaagatcgaa gggcttttgc taaccggtgg
     1201 aaatacgtat tacatcattg tcgatggata cagttcgggc gattttggca actatgacct
     1261 ttcggtcacc cagtccgaca acactcccca gtgcatcata ggaattaatt ttatctgccc
     1321 ggcctgggcc gttgacgaac cggaggattg cggtcaggat gagaacggcg gctgcaatat
     1381 gattcccggg aatgaaagct gggaatcggt tccgtctgcc ggggctacct attgcggcag
     1441 tttatttgcc gacaatggag acaaggactc agactggtat gagctcgttt tgtcagaacc
     1501 ttcgtcagtg gtcctgactg ccaatgcaga acagcaaatt tcttacggcc tggccgaaac
     1561 cacaaccccc ggcgcaccga cctgcattac tctgaccgga gacacggagc ccggggctgt
     1621 ggccggccct tgcagcgaaa catccgtgga cctgggcacc cttgcgcccg ggacttactg
     1681 gttggttatc gaaatgaccg ccaccagcgg tttcccgtgt agcaatcact acgtcttcga
     1741 tatcgatgtc aaccccgtcc cctgcccgcc ccctgatttt ctcactgcta cagggatcac
     1801 ctcttttggt gccacactgg gctggtttga gaccggcaca gcagcatcct gggaatacca
     1861 gtatggcgtg agcggcttta ccccggcagg ttcgggcatt tcgacggttg ccaatccggt
     1921 tgccgttacg gggctggcga tcagcaccgc ttatgatttc tatgccaggg cggtttgcgg
     1981 gcccggtatc tacagcgcct ggtcgggacc ttacaccttc acaacgctat gcggtacaat
     2041 aaactcaatc ccctggtcgg aaggatttga gtccggctgg ccgccggcat gctggaccga
     2101 cgataatgcc ttacccggct acggctggga ccatggcgtt tacggctcgc ccgggagcgg
     2161 ctcgaagtgg gcttacagca acctttcggg ttccgtgctt acctctcctt ccatcagcct
     2221 gtcagccgat gcttacctgt tcttcagtta ccgggctgag aacaaggatt tccccaggaa
     2281 tatatctgta aaaattaaca atgatgaagt tttccaggtt aacggtttta cctctgagga
     2341 ttattccgtc ggtgccgttt cactggctac ctatattggg caaaccgttg cgattgcgtt
     2401 cgaaacaact gcaggaagcg gcgggcagga cttcggcatt tgcctcgacg atgttacggt
     2461 gaagttcgct gtaaaatgga ccggaaattt gggcactgcc tggagcaatc ccggtaactg
     2521 gtcaacctct tccatcccag ggatcaacga tgtggtggtg gtcccttccg tgccatccgg
     2581 aggcaacttc ccggaagtcg gcagcggcat aacggcaacc tgttacttcc tggaactggc
     2641 acctggcgca actctaaaag ttaaaaccgg cggcaccctg aatgttttga attattgatc
     2701 aaactctctt tcattccttg ttcagtttga ccatgcaggg ctttgggcca atattttttg
     2761 ccaccaaaaa acgaatacac aaaactccac taaaggattt agcagactgc caatccgcgt
     2821 gaatccgtta aatccgcgtc atccgtgtgc tatttaattg aaagtagatt ccacaaaata
     2881 tcataaataa tcaaaatatc aggccatatt tctaatatat agtcgtattt ttacgccata
     2941 tttttaaact atgtataaaa gagatattga aaatacaatt aaagaatggc tgccgggtaa
     3001 agaagtcgtt attttaaacg ggcctcgtca gtcgggcaaa acaacacttc tcaccatgtt
     3061 gttcgaccaa aatcctga
//
LOCUS       DAOPNH010000339         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_38680_length_3073_cov_4.017893, whole genome shotgun sequence.
ACCESSION   DAOPNH010000339 DAOPNH010000000
VERSION     DAOPNH010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38680_length_3073_cov_4.017893"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKZ74_13655"
     CDS             <1..1371
                     /locus_tag="PKZ74_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93847.1"
                     /translation="AGTMAYRQAGLLFSDGTIRSAADPAKDIFFASDPTGLMPNSDFY
                     IVVYHRNHMPVMSGAPVNIPNSAMLNLTDLTQAYGYIPASAVEQPLITLETGVYGMIA
                     GDHYGAIPAGEGWSYFDYPDGWLYYSGNPNDRDPILAGIVSVTGLPYINGVINNVYFK
                     EDLTMNRQVKYSGSGNDPRLIIQNLIELKGSNVLTNFYESKVPGWAIPHVKKEGLLSM
                     TGPLDIGLYEAPRELLINITANETFTGAVVSNIQFILCWDESNAEAGRLIAGYQGDFL
                     LEPQGDPVKFAGKMYQVFATVEPVALPDPLNSGEQVTVLRFANSDAAPVAANGLAIAD
                     NAWTLANNGGYYIAVWGAGQTGRIVTGALGTDQPGDSGPVVRIYPNPTATGWIYLTTN
                     PAVSGNLAVRLTDLTGRQLRQSQWSVKSGTANAMTFDLEGLSHGAYLLNINGESFSFS
                     ERIIIR"
     gene            1517..>3073
                     /locus_tag="PKZ74_13660"
     CDS             1517..>3073
                     /locus_tag="PKZ74_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93848.1"
                     /translation="MKRRLLVMLIFGLALSSVWSQTTRYVNPDGNCGGSTPCYTTIQA
                     AINASVDGDIIMISAGTFTETLNVGKSLTFIGSGPVSNPTTIITSTANPMIQLSVTNK
                     NFTFQNLIIEGNVTNNGIWAGGSININSLTLKDFIGRNCQVALYLAEYWPGDDPLTTT
                     VSNLSFDNVTMTNNKFIGVYIGKTVLSGTVTNSTFTGNGYSDELPASWQKTGLQFVNF
                     DEASVPHVVVTNSTFSNNGTGASNIERTGLIIYTAYNALSANEIMTVSGCTFTNHPQY
                     AVRIKNGYNVGNTATVNGTFTNNYLDIWFNNIIGTTTSTTLVRNTFLGTRTVGPGPTY
                     DYNTIQAAINAAAAGDIVRVSDGTYNENVTVNKNISLLSVNGAAVTTIDGLNSGGELG
                     TVYLTAGRDGVTIGDTGQGFTIKGIDGTPGLEKAAIYLQGAQTNITIEGNIIEARGDA
                     GLQGEYNAANDNIIIHNNQFTGQTFTGANPAGVGFAAQFDLPNVPRQLVVFGGGSGTT
                     NTQNINFTNNT"
BASE COUNT          819 a          819 c          764 g          671 t
ORIGIN      
        1 gccggaacga tggcctaccg ccaggcaggc cttttgttca gcgacggcac gatccggagc
       61 gcagccgacc ccgccaaaga tatcttcttt gcttccgatc ccaccggttt aatgccgaat
      121 tccgacttct acattgtggt ttatcaccgc aaccacatgc ccgtcatgag cggtgcaccg
      181 gtcaatattc ccaacagcgc catgctgaac ctgaccgatc tcacccaggc ctacggctat
      241 attccggcga gcgctgtcga acagccgctc atcaccctcg agacgggggt ttacggcatg
      301 atcgccggcg accactacgg ggccataccg gcaggcgaag ggtggagtta cttcgattac
      361 cccgatggct ggctttacta cagcggcaac ccgaacgacc gcgacccgat cctggccggg
      421 attgtgagcg tgaccggcct gccctatatc aatggggtga tcaacaatgt ttatttcaaa
      481 gaagacctga cgatgaacag gcaggtcaaa tactcgggat cgggcaacga tccgaggctg
      541 atcattcaga acctgatcga gctgaaggga tccaatgtgt taacgaactt ctatgaatca
      601 aaagtccctg ggtgggctat cccgcacgtt aaaaaagagg gcctactatc catgactggc
      661 cccctggata ttggcctgta cgaagccccc cgggagcttt tgatcaacat aaccgccaac
      721 gagaccttca caggcgccgt ggtgtcgaac atccagttca tcctttgctg ggatgaaagc
      781 aatgcagagg cgggccggtt gatagccgga taccagggcg acttcctgct cgaaccccag
      841 ggagatcccg taaagttcgc cgggaagatg taccaggtgt ttgccacagt cgaaccggtg
      901 gctttgcccg atccgctgaa cagcggcgag caggtgaccg tgctgcggtt tgccaacagt
      961 gatgcggcgc ccgttgcagc caatggactg gccatagccg acaatgcctg gacgctggcc
     1021 aacaacggcg gatattatat cgctgtctgg ggtgccggcc agaccggccg tatcgtgacc
     1081 ggagcactgg gcacagacca gcccggcgat tccgggccgg tggtccgtat ctatcctaat
     1141 ccaacggcga ccggctggat ctacctaacg acaaatccgg cggtttccgg aaacctggcc
     1201 gtcaggctga ccgatctgac cggcaggcag ctccgtcaga gccagtggag tgtaaaatca
     1261 gggacggcca atgccatgac tttcgacctg gaaggcctga gccatggagc ttatctgctg
     1321 aatatcaacg gcgaatcctt cagtttctcc gaaaggatca ttatccggta gccccccgga
     1381 ccggataaca ggcctgcctc tcttagcccg ctgcaggcga ttattctgca gcgggcgacg
     1441 gaggggtatc tgaatctgtg cactgacaca aaccaattaa ataacaacat tttcaaatct
     1501 caaaaaacaa aaaattatga aaagaagatt actggtaatg ctaattttcg gtttagcatt
     1561 gagtagtgtc tggtcgcaaa cgacccgata cgtaaacccc gacggcaact gcggaggcag
     1621 cactccctgt tacacgacca tccaggctgc cattaatgca tctgttgatg gagacatcat
     1681 aatgatttct gcgggaacct tcacagaaac gctgaatgta ggtaaatcgc tgactttcat
     1741 cggatccggg ccggtatcca atccgaccac gatcattaca tcgacagcca atcccatgat
     1801 ccagttatca gtcaccaata aaaatttcac ctttcagaac ctgatcattg aagggaatgt
     1861 gacaaataac ggtatttggg ccggtggctc catcaacatt aattcactga cattaaaaga
     1921 tttcatcgga agaaattgcc aggtggcact ttacctggca gaatactggc ccggtgatga
     1981 tcctctgacc accacagtca gcaatttaag ctttgataat gtcactatga caaacaataa
     2041 attcatcggc gtctatatcg ggaaaacagt actgtcaggg acagtgacca attcgacatt
     2101 taccggtaat ggatattcgg atgaattacc agctagctgg caaaaaaccg gcctgcaatt
     2161 tgtaaacttt gatgaagcat cggtacctca tgtagtggtc acaaattcca ctttttccaa
     2221 taacggaaca ggcgcttcca atatcgaaag gacggggctc atcatctata cagcctacaa
     2281 tgcgctcagt gccaatgaga tcatgactgt atccggctgc acctttacca atcatcctca
     2341 atatgcagtg aggataaaga atgggtataa tgtaggaaat acagcaaccg taaatggaac
     2401 tttcacgaat aactacctgg atatctggtt taataatata atcgggacca ccacatccac
     2461 tactcttgtt agaaatacat ttttggggac caggactgtt ggtccgggtc ctacttatga
     2521 ttacaacacg atccaggccg ccatcaatgc cgccgcagcc ggagatatcg tcagggtttc
     2581 tgacggcacc tacaatgaaa acgtgaccgt taacaagaat atcagcctgt tatcggtcaa
     2641 cggtgcggct gtaaccacta tcgacgggtt gaattccggc ggtgaactgg gaacggttta
     2701 cctgacggca ggaagggatg gcgtgaccat tggggatacc ggccagggct ttaccatcaa
     2761 aggaattgac ggtacacccg gacttgaaaa agcggcaatt tatctccagg gtgcccaaac
     2821 caatatcacc attgaaggaa acatcattga agcaagaggt gacgccggct tacaaggtga
     2881 gtacaatgct gccaacgaca acatcatcat ccataataac cagttcaccg gacagacctt
     2941 tacaggagct aatcccgcgg gggttgggtt tgctgctcaa tttgacctgc cgaatgttcc
     3001 caggcaactg gttgtatttg gcggcggatc aggcaccaca aacacccaga acatcaattt
     3061 caccaataac act
//
LOCUS       DAOPNH010000340         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_39188_length_3047_cov_4.194519, whole genome shotgun sequence.
ACCESSION   DAOPNH010000340 DAOPNH010000000
VERSION     DAOPNH010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39188_length_3047_cov_4.194519"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PKZ74_13665"
     CDS             <1..1284
                     /locus_tag="PKZ74_13665"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HPM93849.1"
                     /translation="EDAIGSNIADFLIKPVKPNQILLSLKKNLENKKLFSEKTTSIYQ
                     QEFRNISMDISNNPDYSGWMDIYRNLTRWELELEKSNDASMQEVLQHQKSEANQVFSK
                     YVENNYLNWINGRSRTYPVMSPNLFKDKVFPLLDSNESLFVILIDNLRYDQWKAIQPI
                     LEEYFRIDREEMYYSILPTTTQYARNALFAGLMPLEIERKYPKYWVAEEDEGTKNQFE
                     AELLGEYLKRYGRDIKYSYNKILNLTAGRKLVETLPNLMSNKLSVIVYNFVDMLSHAR
                     TEMEIIRELADDEKAYRSLMVSWFNNSPLLDIIKYLAEKKVNVVITTDHGSVKAQDPI
                     KVIGDRNTTTNLRYKSGRSLNYSPKEVFEVRNPHDAYLPRTNVSSSFIFCRRNDFFAY
                     PNNYNYYVNYYRNTFQHGGISLEEMLVPVAYLRAK"
     gene            complement(1297..1998)
                     /locus_tag="PKZ74_13670"
                     /pseudo
     CDS             complement(1297..1998)
                     /locus_tag="PKZ74_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
     gene            complement(2014..>3047)
                     /locus_tag="PKZ74_13675"
     CDS             complement(2014..>3047)
                     /locus_tag="PKZ74_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93850.1"
                     /translation="VHFTDLSAYCPGTWQWSFSPVSVEYREGTDEFSRNPVVEFRESG
                     PYDVTLSVTNNNGESVLTRPGYIQSGGYNLPFVETFESGTLEERYWTVLNPDFDYTWT
                     DYLIEETGNHTVRMKNYAYFNMGERDQLISPYFNFSELSNVYLTFDHAYAQRFTLRDS
                     LIIYISGECEGAWTRVWANGPDGNGIFETAPATPYEFVPLQNDDWCGLGWGADCFTID
                     LSDWAGENNVRIIFEGYNNLGNNLFLDNITVSNTTGHQDIKPAIGSFTLFPNPGNGIF
                     TLRSSGITGIIQLDILNSQGQMVLSESIVNHSNTYEETLDLTSLAKGIYIVRLAGSTG
                     LQVKKLIIR"
BASE COUNT          832 a          702 c          728 g          785 t
ORIGIN      
        1 gaggatgcca tcggttcgaa tatagcggat ttcctgatca aaccggttaa gcccaaccaa
       61 atactcctga gcctgaaaaa gaacctggag aataaaaagc tattcagcga aaaaaccact
      121 tcaatttacc agcaggaatt caggaatata agtatggata tatccaataa tccggattat
      181 tccggatgga tggatattta ccgcaacctg acccggtggg aactggagct ggagaaatcg
      241 aatgacgcca gtatgcagga ggttcttcag catcagaaaa gcgaggcaaa ccaggttttt
      301 tccaaatatg tggagaacaa ttaccttaac tggattaacg gtcgttcgcg gacctacccg
      361 gttatgtcgc ctaatttgtt taaagacaag gtctttccat tgcttgacag caatgagtca
      421 ctgttcgtta tccttattga taacctgcgg tacgatcagt ggaaggccat acagccgata
      481 cttgaggaat atttcaggat cgacagggag gagatgtact acagcattct tccgacgaca
      541 acgcaatatg cccggaatgc attgtttgca ggcctaatgc ccctggagat cgaacgcaag
      601 taccccaaat actgggttgc ggaagaggat gaagggacca aaaaccagtt tgaggcggaa
      661 ttactgggtg aatacctgaa aaggtacggg cgtgatatca aatattccta caataaaatc
      721 cttaacctga ccgccggccg gaagctggtg gaaactcttc ctaacctgat gtcgaacaag
      781 ctatctgtga tcgtttacaa ctttgtagat atgctctccc atgcccggac tgaaatggag
      841 atcatcaggg aattggcgga tgatgaaaaa gcctaccggt cgctgatggt ttcgtggttt
      901 aataattcac cgttgctcga tattatcaaa tacctggcag aaaagaaggt aaacgtggtt
      961 attacgactg accatggttc tgtcaaagcg caggatccga ttaaggtgat cggagaccgg
     1021 aatacgacaa caaatcttcg gtataaatca ggcagaagcc tgaactacag cccgaaagaa
     1081 gtattcgagg tgcgtaatcc gcatgatgca tatcttccca ggaccaatgt cagttcatct
     1141 ttcatattct gccggagaaa tgatttcttt gcctatccca acaattacaa ttactatgtg
     1201 aattattaca ggaacacctt tcagcatggc gggatctctc tggaagagat gctggtacct
     1261 gtagcttatc tcagggcgaa ataggggaag ggataattcc tggtttaaca gccgtatcgt
     1321 atggcacgca accagggctg cggtttcggt gcgcagtcgg caatcgccga gcgaaacgca
     1381 ctgaaaacca tgctctgaag ccagtttcaa ttcctgttga ctgaaatcac cttcgggccc
     1441 gatcaaaata acagaatggg aatccttttt ataggctctt tttaataaaa tcggattttc
     1501 tccttcgaga tgggctataa accggccctc aggatgatcc cgggcgataa atttgttaaa
     1561 atcggtcaat tcgttcagct tcggtctgta agctttgtaa gattgtttca tggcagccgt
     1621 gatcactttc tcaaggcgct cactccggag ctgccggcgt tcggaatggt cgcacaccag
     1681 cggggtgatc tcatcaatac cgatctcggt tgatttctcc aggaaccatt ccatgcggtc
     1741 aatatttttt gtcggggcca cggcaatatg cacatagccg gccatgcgcc cataggcttc
     1801 ttgttttccg gtaatctcaa cggtgatcct tttgcttttt gaatcgacga tcctggattc
     1861 ataaaggttt cccaatccgt ctgtcatatg gatcacagcg ccttcgctaa ggcggaggac
     1921 cattttacaa tggcgtgatt cttcgtcatc gagggtatat aggcctgctg ttacgccggg
     1981 cgcatagaac aggttcatga cggcacgact gaattaccgg atgatcagtt ttttgacctg
     2041 caaaccggta ctacccgcaa gcctgacgat atagatgccc ttggccagag atgtgaggtc
     2101 aagcgtctct tcataagtat tactatgatt tacaatactt tctgaaagaa ccatttgtcc
     2161 ctgcgaatta aggatatcca actggataat gcctgttata ccggaagaac gcaatgtaaa
     2221 gataccatta cccggattgg ggaagagggt aaaacttccg atcgcgggct ttatatcctg
     2281 gtgaccggtc gtgttagaga cggtaatatt atctaaaaat agattgtttc ccaggttgtt
     2341 atagccttca aagataatcc tgacgttatt ttcgccggcc caatcgctca ggtcgatcgt
     2401 aaaacagtcg gctccccagc ccagtccgca ccagtcatca ttttgaagcg gaacgaactc
     2461 atagggtgtt gccggcgctg tttcaaaaat accgttgccg tccggaccat tggcccaaac
     2521 gcgtgtccat gccccttcac attcacctga aatataaatg atcagtgaat ctctcagtgt
     2581 aaaccgctgg gcataagcat gatcgaatgt cagataaaca ttgctcagtt cactgaaatt
     2641 gaagtagggg ctgatcaact ggtcgcgctc gcccatgttg aaataggcgt aattcttcat
     2701 cctgacggta tgatttccag tttcttcgat caggtagtcg gtccatgtgt aatcgaaatc
     2761 gggattgagc acggtccagt agcgctcttc cagggtgccg gattcaaatg tctctacgaa
     2821 aggcaggtta tatcctcccg actgtatata gccgggccgg gtcagtaccg attcgccgtt
     2881 gttgttggtt accgacaatg taacatcata cggacccgat tcacggaatt caacgaccgg
     2941 gttcctggaa aactcatcgg taccttcccg gtattcaacc gaaacaggtg aaaacgacca
     3001 ttgccaggtg ccggggcaat aagctgaaag atcggtaaaa tgaacgg
//
LOCUS       DAOPNH010000341         3045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_39224_length_3045_cov_4.834783, whole genome shotgun sequence.
ACCESSION   DAOPNH010000341 DAOPNH010000000
VERSION     DAOPNH010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39224_length_3045_cov_4.834783"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2992)
                     /gene="uvrA"
                     /locus_tag="PKZ74_13680"
     CDS             complement(122..2992)
                     /gene="uvrA"
                     /locus_tag="PKZ74_13680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPM93851.1"
                     /translation="MFREFDDSENIDNRIGTDYLEVFGARVHNLQNIDVRIPRNSLVV
                     VTGLSGSGKSSLAFDTIYAEGQRRYMETFSIYARQFIGNLERPDVDKISGLSPVVSIE
                     QKSVNKNPRSTVGTITEVYDFLRLLYARIGEAYSWVTGEKMVRYSEDQIIRLITEKYQ
                     NQPLVILAPVVRGRKGHYRELFENIRKMGYLKVRVDGEIKDLVFGMQADRFKVHDIEI
                     VIDRIRMDGTKGTDLRLVQSVQTAMKHGKDALILLQPDTNEVRPFSSKLMCPVSGISY
                     DEPEPNTFSFNSPYGACPNCNGLGSITEVDLKKILPDPKLSIKKGGIAPLGEYKNNWI
                     FSQMEAIGYKYGFTLDTPVKDIPDEAISTILYGSDETVHVKKEYLGVTSSYTLSFEGI
                     INFITNQLNDTTSEGIHRWAISFMNKVQCPVCYGSRLKKESLHFKIDEKSIADISRMD
                     LVTMGDWIDSLEAKLDERQRMISHDIIREITNRLQFLLNVGLDYLSLERSARSLSGGE
                     SQRIRLATQIGSRLVDVLYILDEPSIGLHQRDNVRLINALKELRDIGNSVIVVEHDRE
                     MIESADWVVDIGPGAGRHGGKIVGAGKPGELKDCTTITCDYLSGRKKISVPEKRREGN
                     GQNIILEGASGNNLKNVTLLLPLGKFICVTGVSGSGKSTLINETLYPILSSHFYHSSQ
                     KPLPYRSVAGLEHIDKVIEIDQSPIGRTPRSNPATYTKVFDEIRKLFVQIPEAKIRGY
                     SPGRFSFNVKGGRCETCKGAGLRVIEMNFLPDVYVICESCNGLRYNRETLEVRYKGKS
                     INDVLEMTIDQAVDFFSNIPSIIQKIKTLQDVGLGYITLGQQSTTLSGGEAQRVKLAA
                     ELAKRDTGKTLYILDEPTTGLHFEDVKVLLEVLNRLIERGNTILVIEHNMDVIKVADH
                     IIDLGPEGGERGGRIICEGTPEEISHHPGSHTARFLKLELSR"
BASE COUNT          701 a          799 c          728 g          817 t
ORIGIN      
        1 accgccgcca ctcagtacga gcccgtattt gtgttttact ttctgcatta acacttaatc
       61 acacttcaat aacgcaaagt tatgcaaagg aagaccaaag gtaagcaaaa gagggggtaa
      121 attaccttga caattccagt ttgagaaacc tggccgtatg gcttccggga tgatggctaa
      181 tttcttccgg ggtaccttca caaatgatcc ggccgccacg ctcaccgcct tctggaccaa
      241 ggtcgataat gtggtcggcc actttaatca catccatatt gtgttcgatc accaaaatgg
      301 tatttcctct ttcgatcagc ctgttcaaca cctcaagcag caccttcaca tcttcaaaat
      361 gaagtccggt cgtaggctca tccaggatat aaagcgtttt gcccgtatcg cgcttggcca
      421 gctcggccgc taacttgacc cgttgcgcct caccccccga gagcgtggtc gattgttggc
      481 ctagcgtgat ataacccagg cccacatcct gcaaagtttt gatcttctgt ataatagaag
      541 gaatgttact aaagaaatca accgcctggt caatggtcat ctccaggaca tcattgatcg
      601 acttgccttt atacctgact tcgagcgttt cgcggttgta tctcagtccg ttgcaagact
      661 cacaaatgac atatacatcc ggaaggaaat tcatctcgat aacccgcagg ccggcccctt
      721 tgcatgtctc gcaccgcccg cctttgacgt tgaatgagaa cctgccgggt gaataacctc
      781 ttattttggc ttccgggatc tggacaaaga gttttcttat ctcatcgaaa acctttgtgt
      841 aagtagcggg attcgatctc ggcgtacggc cgatcggcga ctgatcgatc tcgatgactt
      901 tatcgatatg ctccagcccg gcaacagaac ggtatggaag gggcttctgg ctggaatggt
      961 agaaatggct gctcaggata gggtagaggg tttcgttgat cagcgtagat tttccgcttc
     1021 ccgacacacc cgtaacacag atgaattttc ccagtggaag taggagcgta acatttttca
     1081 gattatttcc cgaggcacct tccaatatta tgttttggcc gtttccttca cggcgctttt
     1141 ccgggaccga aatttttttc ctgccggaga ggtagtcgca ggtaatggtt gtacaatcct
     1201 ttaactcacc cggtttaccg gcgcctacaa ttttaccgcc atggcgcccg gctccgggcc
     1261 caatgtcgac cacccaatcg gccgattcga tcatttcccg gtcgtgttcc acgacgatca
     1321 ccgaattacc gatatcgcgc agttccttga gggcattgat cagcctgaca ttatcccttt
     1381 ggtgcagccc gatgctgggt tcatccagga tatacaacac atcaaccagc cgggaaccga
     1441 tctgggttgc cagccggatg cgctgcgact caccgccgga aaggctcctg gccgaccgtt
     1501 ccagggagag gtagtcaaga ccgacattca gcaggaactg cagcctgttc gtgatctcgc
     1561 ggatgatgtc gtgtgaaatc atccgctggc gttcatctaa tttggcttcc aaagaatcga
     1621 tccaatcgcc catcgtcacc aggtccatcc ggctgatatc tgcaatactt ttctcatcga
     1681 tcttaaaatg gagcgactct tttttcaacc gggagccata gcatacaggg cattgaacct
     1741 tattcatgaa cgagatggcc caacggtgta ttccttccga tgtggtatca ttcaactgat
     1801 tggtaatgaa gttgatgatc ccttcgaaac tcagggtgta tgatgatgtt actccaagat
     1861 attccttttt gacatgcacc gtttcatcgg atccataaag gatcgtggag atagcttcat
     1921 ccggaatgtc ctttacaggt gtatcaaggg taaatccgta tttgtatccg atcgcctcca
     1981 tctggctgaa tatccagttg ttcttgtatt cgccgagcgg ggcgatcccc ccttttttta
     2041 tgcttagttt gggatcgggc agaattttct tcaggtcgac ctccgtaatg ctgcccagtc
     2101 cgttgcaatt cgggcaggca ccgtaaggcg aattaaagga gaatgtgttc ggttcgggtt
     2161 catcgtagga tatccctgac accgggcaca tcaatttact gctgaatggc cgtacctcat
     2221 tggtgtcggg ctgtaacagg atcagcgcat ctttgccgtg tttcatggcg gtctggaccg
     2281 attgtaccag gcgcaggtcg gtgcctttgg tgccatccat cctgatccgg tcgatcacga
     2341 tttcgatgtc gtgtaccttg aaccggtcgg cctgcatacc gaaaacaaga tctttgatct
     2401 ccccgtcgac ccgaaccttc agatacccca tcttgcgaat gttctcaaac agctcccggt
     2461 agtgcccttt gcggcctctg accaccggtg ccaggatcac caggggctgg ttctggtact
     2521 tttccgtgat gagccggatg atctggtctt ccgaataacg gaccatcttt tcaccggtca
     2581 cccatgagta ggcttccccg atacgggcat acagcaggcg gagaaaatca taaacctccg
     2641 taatggtacc tacagtagag cgggggtttt tattgaccga tttctgctcg atcgaaacga
     2701 cgggcgagag gccgctgatc ttgtcgacat cgggccgttc caggttaccg atgaattgcc
     2761 tggcgtatat agaaaatgtc tccatatacc ggcgctgccc ttcggcgtag atcgtgtcga
     2821 atgccaggct tgacttgcca cttccgctga gaccggttac aacaacaaga gagttacggg
     2881 ggatcctgac gtcgatattc tgcaggttat ggacacgggc gccgaatact tcaagataat
     2941 cggtgcctat ccggttgtca atattctcac tatcatcgaa ttccctgaac attcttgagg
     3001 ataaattgat ttacaattta cagtttacag tttacaatct ataat
//
LOCUS       DAOPNH010000342         3044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_39237_length_3044_cov_7.696554, whole genome shotgun sequence.
ACCESSION   DAOPNH010000342 DAOPNH010000000
VERSION     DAOPNH010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39237_length_3044_cov_7.696554"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1037)
                     /gene="gldA"
                     /locus_tag="PKZ74_13685"
     CDS             complement(126..1037)
                     /gene="gldA"
                     /locus_tag="PKZ74_13685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HPM93852.1"
                     /translation="MSIEVSNVIKIYDTQKALDNVSMSVKPGEIVGLLGPNGAGKSTL
                     MKIITCFIPPTEGAATICGYDIFENSLKVREKVGYLPEHNPLYLDMYVGEYLEFVAGI
                     HKIKSNVKSRIGEMIELTGLTDERKKKIGALSKGYRQRVGLAQALIHDPEVLILDEPT
                     SGLDPNQISEIRHLIRSVGATKTVMLSTHIMQEVEAICDRAIIIHKGRIVADDATRNL
                     TTGIKNRNRITVEFSGKIDSQDFKRISGITGIERKSEKVWALETAEGRDIREELFSLA
                     VSKGIAILSMNREELRLEEVFQILTQG"
     gene            1168..>3044
                     /locus_tag="PKZ74_13690"
     CDS             1168..>3044
                     /locus_tag="PKZ74_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93853.1"
                     /translation="MFLIIHCTVVNGQNESIIISSDSINPLTDNTYFNASLFGHLPFT
                     GTSDIFQLNPALYRNSAAGTFLIDGFSTSGDYTLFDGIPMDFIEEMPLRLIGEVKFND
                     FHDFLTCNNTITGYARLEPLRQSDSLTIRLEPSTRIIHGQFNDADLQLIISGPLIKQT
                     PGKNNSTSLNFTLAGRLFSASDPNPSYIGRDQASADYLDYLADESLRPSEFSGTYPNA
                     CFTHATNAVSSYFNSNAAKKGFSLYGSVSAEAGNGMFLKLGTYTVKKKETIPVYDNFF
                     FNQDKNPDQSTLYSNNYITLGQILKSGRSTRFTYQVQGNYSIQKNKIEDPDYGDDFFK
                     YGYVGKFETFKRPTYELGSIQVGDTYYEQALILNSWDYDTLVDFSPGEINPGLAAYTS
                     SYFDIFVGEPDNHYGNKDQIQLGGGILNGQNPDKVYGLWNNTGTVYNYYSKEDNRRIN
                     IAASGMLELDNHKIKIGFQFRRDSYSSYQANPVGLWGLMRSMTNFHINELDLSNPEVH
                     EGGLWDSVFFYRKYDAASQHYFDRNLREALGLDPDGTDFIDIDSYDFITSTINYFDKE
                     GLKHTIDVGKELFSLDMFSADELLNDGIASFVTYSGYDHLGNKNGNKVAFEDFFTATD
                     G"
BASE COUNT          788 a          693 c          665 g          898 t
ORIGIN      
        1 gagggttgga accgacggct tcagtcgtcg gactacctaa aaaaagtgaa tatatagcct
       61 tttgagacac cctcttgcac attatttttt taagggcaca aatctatgaa taaaagcaat
      121 atgaatcagc cttgagtcag aatctggaaa acctcttcca gccgcagctc ttcccggttc
      181 atggaaagta tggcgatgcc cttgcttacg gccaggctga acaattcctc gcggatatct
      241 cttccttcgg ccgtttccag agcccagact ttttcgcttt ttctttcgat gccggtaatc
      301 ccgcttatcc gtttaaaatc ctggctgtcg attttaccgc tgaactctac ggtgatccgg
      361 ttcctgtttt taatgccggt ggtgaggttc ctggttgcat cgtcggccac gatcctgcct
      421 ttatggatga tgatggcccg gtcgcagatc gcttccacct cctgcatgat gtgcgtcgac
      481 agcatcaccg tttttgtcgc cccgaccgac cggatcaggt ggcgtatctc gctgatctgg
      541 ttgggatcaa ggcccgacgt cggctcgtcg aggatgagca cctccgggtc gtggatcagc
      601 gcctgcgcca gcccgacgcg ctggcggtac cctttcgaca aagccccgat cttcttcttc
      661 cgctcatcgg tcagacccgt cagttcgatc atttcaccga tcctggactt tacattgctt
      721 ttgatcttgt ggattccggc gacgaattcc agatattcgc cgacatacat gtcgagatag
      781 agcgggttgt gttccggcag gtagccgact ttttccctca ctttcagcga attctcgaaa
      841 atatcgtacc cgcagatggt agcagcccct tccgtcggcg ggataaagca ggtaatgatc
      901 ttcatcagcg tggatttccc ggcgccgttc ggaccgagca gtccgacaat ctcaccgggt
      961 ttgacactca tggaaacatt gtccagggct ttctgcgtat cgtaaatttt aattacattg
     1021 ctgacttcaa tcgacatatt gctttttcga atagggatgc cgaaattagt aaaatattta
     1081 tatttatgct cttgaaatta ttaacctttt cctttaagtt ctttcggttt tatgaaaaaa
     1141 atcaaattta ttggatcgat tttgatcatg tttttgatca ttcattgcac agttgttaac
     1201 ggtcaaaatg aatcaataat tatttcatcc gatagcataa atccgttaac agataatact
     1261 tacttcaatg ccagcttatt cgggcatctt ccttttaccg gaacgtctga tatttttcag
     1321 ttaaatccgg ctctttaccg gaacagcgcc gccggaacct ttttaataga tggattcagc
     1381 acttcaggcg attatacctt atttgacggg atcccgatgg attttatcga agaaatgccg
     1441 ctcagactga tcggtgaagt taagtttaat gattttcatg attttctcac ctgcaacaat
     1501 accatcaccg gttatgcccg gcttgagccg ctccggcaat ccgacagcct gaccatccgc
     1561 ctggaaccat ccacccgcat aattcatggg cagttcaatg acgcagacct gcagttaatc
     1621 atttccggtc cgttaatcaa acaaacaccc ggaaaaaaca attccacttc actgaacttt
     1681 acgctggcag gccgcttgtt ttctgcatct gatccaaacc cttcgtatat cgggagagac
     1741 caggcatctg cagactacct tgactatctg gcggatgaat cccttcgccc ttctgaattt
     1801 agtggtactt acccaaacgc ttgtttcacc catgccacca atgctgtttc gtcttatttt
     1861 aattcaaatg ctgctaaaaa aggattttcg ctatatggtt ctgtgagtgc ggaagctgga
     1921 aatgggatgt ttctgaaatt gggcacatac acggtgaaaa aaaaggaaac cattccggtc
     1981 tatgacaatt ttttctttaa ccaggataag aatccagatc aatcaacctt atattcaaac
     2041 aattacatta ctctcggtca gattcttaaa tccgggagat caacccggtt tacctatcag
     2101 gtgcaaggta attactcgat tcagaagaac aagattgaag atcccgacta tggcgatgat
     2161 tttttcaaat acggatatgt tggaaaattt gagacattca aaaggccaac ttatgagtta
     2221 ggctctatcc aggtcggtga tacgtattat gaacaggcat tgatactgaa ttcctgggac
     2281 tacgataccc tggtcgattt ttcaccgggt gaaataaatc cgggattagc cgcttatact
     2341 tcgtcgtatt ttgacatctt tgttggtgaa ccggataatc actacggaaa caaagaccag
     2401 atccaattgg gaggagggat actgaatggt cagaatcctg acaaggttta tggtctgtgg
     2461 aataataccg gcactgttta taattattat tcaaaagagg ataacaggag gatcaatatt
     2521 gctgcgagtg gtatgctgga gcttgacaac cataagataa aaattgggtt tcagttccga
     2581 agagacagtt attcttccta tcaggccaac ccggtgggtc tgtggggttt aatgcgctct
     2641 atgaccaatt ttcatatcaa cgaactcgat ttgtcaaatc ctgaagtcca tgaaggtgga
     2701 ctttgggact ccgttttctt ctacaggaaa tatgatgcag ccagccaaca ttactttgat
     2761 aggaacctga gggaagcgct tgggcttgac ccggacggaa cagattttat cgatatcgat
     2821 tcctatgatt tcatcacaag tacaataaat tattttgaca aagaaggttt aaaacacacg
     2881 attgatgtcg gaaaggaatt gttttccctg gatatgtttt ctgctgatga gctgttgaac
     2941 gatggcattg cttcctttgt tacttacagc ggatatgatc atttaggcaa taagaacggg
     3001 aataaagttg cttttgagga ttttttcaca gctacggatg ggga
//
LOCUS       DAOPNH010000343         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_39416_length_3035_cov_3.757383, whole genome shotgun sequence.
ACCESSION   DAOPNH010000343 DAOPNH010000000
VERSION     DAOPNH010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39416_length_3035_cov_3.757383"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PKZ74_13695"
     CDS             complement(<1..1538)
                     /locus_tag="PKZ74_13695"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPM93854.1"
                     /translation="MVQEILIGGGVETFNIVYTGNNISRGKFWGGPGNIGIEEGIILT
                     SGAVTVAPGPNNSGSAGSNSGQGSDPDLVLLSGTSINDRCLLEFDFIPQSTVVTFKYC
                     FGSEEYHEYVDQFNDAFGFFISGPGISGPYSNNSKNIALIPLTNIPVTINTVNCGNPY
                     NCQEECDNCQYFVNNTQNFVQYDAFTKPLVAWANVIPCETYHIKLAIGDGIDHAYDSG
                     VFLEKNSFSSVGIASQEEYSEPEFEFAIEGCNNVTIDFQLSVQPDADFYMPLMISGSA
                     VNGVDYEEISDSLLFPQGYTQASLDIITIADGISEWNENIRIVYNSSLCGIDYDTINI
                     GLQDYKLNLNMTPDTTINCATDAYIGVNSIIGFGPYDFVWSTGDTTPFITVSPLITTT
                     YYVTCLALCDSLTVDSVTVFVDGPESHAGQDQSIPYGTTTTLQGSASQGSGEYTYSWE
                     PAALLVDPASPTPTTLQMEQTTQFTLTVTDLAGNCQDLDAMMLYVTGGPLNVGPMASP
                     AEICV"
     gene            1872..>3035
                     /locus_tag="PKZ74_13700"
     CDS             1872..>3035
                     /locus_tag="PKZ74_13700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPM93855.1"
                     /translation="MKDIKDYIRENKERFLEELFGLIRIPSISSEAARKEDMNSAAEY
                     WKKTLLKAGIAKAEIIPTEGNPVVYGELIIDPGKPTVLVYAHYDVMPVDPVELWNSPP
                     FEPEIRDGKIFARGADDDKGQSFMQAKAFEYLVKSGTLPCNVKFMIEGEEEIGSVNLG
                     KFCDDYKEMLKADVILVSDTSMIAPDIPSITSGLRGLSYMEVEVTGPNKDLHSGLFGG
                     AVANPANILVDMIASLKDKNQRITIPGFYDDVVEISTEERQKMARAPFNLENYKKALD
                     IDEVCGEAGFTTPERTGIRPTLDINGIWGGYTGEGAKTVIPSKAYAKISMRLVPNQDH
                     HRISRLFEEHFKAIAPPYVKVKVTALHGGQGYVSPTDMPAYKAAEKAYMKTYSK"
BASE COUNT          888 a          687 c          725 g          735 t
ORIGIN      
        1 acacaaattt cggctggtga agccataggg cctacgttca acgggccacc ggtcacatat
       61 agcatcatgg catccagatc ctggcaatta ccggcaagat cggttacggt cagtgtgaac
      121 tgcgttgtct gctccatctg taaggtagtg ggcgtcgggc tggcaggatc aacaagcaaa
      181 gctgccggtt cccagctata ggtatattca ccggagccct ggctggccga tccttgcagg
      241 gttgtagttg ttccgtacgg aatactctga tcttggcccg catggctttc gggaccgtcg
      301 acaaaaaccg tcacgctatc gacggttaac gaatcacaaa gcgccaggca ggttacataa
      361 tatgttgtcg taatcaaagg gctcaccgtg ataaacggtg tagtatcgcc cgtgctccag
      421 acaaagtcgt agggcccgaa tccaataatg ctgttgacac cgatataggc atctgtggca
      481 cagtttattg tcgtatcagg agtcatgttc agattgagct tataatcctg aagcccgata
      541 ttgatcgtat cataatcaat cccgcaaagt gaagaattat aaactatgcg gatattctca
      601 ttccattcgc tgataccgtc ggcaatggtt attatatcta aagaagcctg cgtatacccc
      661 tgcgggaata ataaagaatc cgatatttct tcatagtcaa ccccattgac ggccgacccg
      721 gaaatcatca gaggcatgta aaaatcagcg tcgggctgga ccgacagttg aaaatcgatc
      781 gtcacattat tgcaaccttc aatggcgaac tcaaattcag gctccgaata ttcctcctgg
      841 gaggcaatgc ctacactgga gaagctgttc ttttcgagga aaaccccgga atcataagca
      901 tgatcgatac catcgccgat ggccagcttg atatggtatg tttcacacgg aatgacattc
      961 gcccaggcta ccaggggttt ggtaaaggcg tcgtactgga caaaattctg ggtattattg
     1021 acgaaatact ggcaattatc gcactcttcc tggcaattat agggattgcc gcaattaacg
     1081 gtgttgattg tcactggaat attggtaagc gggattaaag caatattttt gctgttattg
     1141 gaatagggac ccgagatccc cggtccgctt ataaaaaatc cgaaagcgtc gttaaactga
     1201 tccacatatt cgtggtactc ttccgatccg aaacaatact taaaagtaac gactgttgat
     1261 tgagggataa aatcaaattc aagtaaacac ctatcattaa tagaggtgcc ggagagcagt
     1321 accagatcgg ggtcactgcc ctgtccggag ttggatcccg cgctcccgct gttgttgggt
     1381 cccggagcca ccgtaacggc ccctgaagtc aggattattc cttcctcgat gccgatattt
     1441 ccgggtccac cccagaattt accccgcgaa atattattac cggtgtaaac aatgttgaaa
     1501 gtttcgacac ctcccccgat caatatctcc tgtaccatct gctctggagt aatactgctg
     1561 gaaacctgca gtacttgtgc atttgagcca aaaggaatca aaaatgttgc cggtagccat
     1621 gcaaatacaa aaagtctgga caagtaggtg taaagattcg tcatgctatt taggtataaa
     1681 taattgattt tatgtatagt aatgaaacat cccgcccata gcatgcaaat ataaataaaa
     1741 ataaatcaaa aatatatcaa tgaaaattag ctgaattacc gggactttac gaagggcatc
     1801 tcgctaattt gcaatatatt tgcatcaaag cagttattca aaaatcatta aaaaaagaaa
     1861 aaataattat tatgaaagat attaaagact atatccggga aaataaggaa aggtttctcg
     1921 aggagttatt cggattgatc cgaatcccct ctatcagcag tgaagcggcc aggaaagaag
     1981 atatgaacag tgccgcagaa tattggaaaa agacgttgct caaagcaggc atcgcgaaag
     2041 cggaaataat acccacagaa ggcaacccgg ttgtttatgg cgaactgatc atcgatccgg
     2101 gtaaacctac cgtgctggtg tatgcccatt acgacgttat gccggtggat ccggtagagt
     2161 tgtggaactc gccaccgttt gaaccggaaa tccgggatgg aaaaattttt gcacgcggcg
     2221 ccgatgatga caaagggcag tcgttcatgc aggctaaagc tttcgaatac cttgtgaaat
     2281 cggggacctt gccctgcaac gtcaaattta tgatcgaagg ggaggaagag atcggatcgg
     2341 tcaaccttgg caagttttgc gacgattaca aagagatgct gaaagcagat gtgatcctgg
     2401 tttccgacac cagtatgatc gctcccgaca ttccttccat cacgagcgga ctgcggggtt
     2461 tgagctacat ggaggtggaa gttactggac ccaataaaga ccttcactcg ggccttttcg
     2521 gaggtgctgt agccaatccg gccaatattc ttgtggatat gatagcctct ctgaaagata
     2581 aaaaccagag gatcaccatc ccgggattct acgatgatgt tgtggagata agtacggaag
     2641 agcggcaaaa gatggccagg gccccattca acctggaaaa ctacaaaaaa gccctcgata
     2701 tcgatgaagt ttgcggcgaa gcaggtttta ccaccccgga acgaaccggt atccggccaa
     2761 ctctcgacat caacgggatc tggggcggtt acaccggcga gggtgcaaaa acggttatcc
     2821 cttctaaagc ctacgcgaaa atttctatga ggctcgttcc taaccaggac catcatagga
     2881 tcagcaggct gtttgaagag cactttaagg ctattgcccc gccatatgta aaagtaaaag
     2941 taacggcgct ccatggcggc cagggttatg tttcgccaac cgatatgccg gcatataaag
     3001 cagcagaaaa ggcctatatg aaaacttaca gcaag
//
LOCUS       DAOPNH010000344         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_39776_length_3016_cov_4.925701, whole genome shotgun sequence.
ACCESSION   DAOPNH010000344 DAOPNH010000000
VERSION     DAOPNH010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39776_length_3016_cov_4.925701"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PKZ74_13705"
     CDS             <1..1768
                     /locus_tag="PKZ74_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HPM93856.1"
                     /translation="NIITKKNSLKGLSGIINLSAGTSPDYSGSLLLNYRTRKASYSLG
                     ADFGSREMPMFRESYRESYIGDTTEFLQSNAENLMKRQSISVKGGVDYSITDKNTITA
                     EGSYRFFSMSRGGDTRNENWSSTATDREYYFTDALDESDHPSFQFTLRDIHKFNEKGT
                     ELTTQFTFNQGDDSGEQITQQYYSDSTWSETGAYLYNYRQKTTESEKEFRGDIDFEHN
                     FNDKSKLEAGFQLRIDRTEEDYEYLNFDTLYTSWILDLGQSNQYDFSHDIYALYGTYA
                     TEIKKLGIKAGLRAEYTDRNLHQVTGGGDYPYEKFDLYPSLYLTYKLPYNQQMQLSYS
                     KRVQRPRGHMLNPYTMFSDAFSSFAGNPQLEPEFSHNMELNYQKYFGYSFLTIETYYR
                     LTDNKMTRVQKLNDEGVMMITMENINNDRSLGIEASGNIKANTWLTINPIASLYDYAL
                     NQTENGEIETRRSTNWMASLELAANLKTGTKIRLNGNYDSPSITVDGTRKGTFYTGFS
                     ARQDLLNNNLSITLNIRDIFDSRHMKETSEGENYYITGENWRKAPVFNIALSYKFNNY
                     NRKRSADSFDGDYDVINMNSYF"
     gene            1781..2608
                     /locus_tag="PKZ74_13710"
     CDS             1781..2608
                     /locus_tag="PKZ74_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93857.1"
                     /translation="MKKNILILAAVLFCLALSVNAQKVAYYESKWYPGVVKDNEADLL
                     NLMQYNEKSGMLFLVSNDDSRLYITLVATDKAAIQKIMRFGLTTWIDPEAKGKKRYAI
                     EFPVSSVKLPAPAADPGRGDRKEARMAMMAAKNGLMILQGFAEKNESLEIDPRMDENF
                     NGRFEMMEGGKLSVSLILPLDRMGLSIAGAAGHPVSVAFETGYMDVTGAMPAGGGQPG
                     GGGMHGGGMYGGGMPGGGPPPGAGGQAGSDTQDRPDIGQLASPTRLRIKTVKLAPKP"
     gene            complement(2732..>3016)
                     /locus_tag="PKZ74_13715"
     CDS             complement(2732..>3016)
                     /locus_tag="PKZ74_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93858.1"
                     /translation="VIFVGMDEMNGTGFSIFPIPNDGKFVANIAINGEDVFSIKVYSD
                     LGVKVYERNNIRVNGKAQIPVDLNNPGKGVYTVVLQGDNQTFTRKMLVTR"
BASE COUNT          892 a          689 c          717 g          718 t
ORIGIN      
        1 caacatcatc accaaaaaga acagcctgaa agggctttcg gggatcatca atctcagtgc
       61 cggcaccagc cccgactatt caggcagttt gttgttgaac taccggacca ggaaagccag
      121 ctattcattg ggcgctgatt ttggaagccg tgaaatgccc atgttccggg aaagctacag
      181 agaatcatat ataggagata caacggaatt cctgcaaagt aatgccgaaa acctgatgaa
      241 aaggcaaagc atcagcgtta agggaggtgt cgattacagt attactgaca agaacacgat
      301 tactgcggaa ggaagttacc gcttcttcag catgtcgagg ggcggggata ccagaaatga
      361 aaactggagt tctactgcaa ccgatcgcga atactatttt accgatgccc tcgatgaatc
      421 cgatcacccc tctttccagt tcactctcag ggatatccat aaattcaatg agaaagggac
      481 cgaactgact acacagttca cgtttaacca gggtgatgat tccggggaac agattacaca
      541 gcagtattat tcggattcaa cctggagtga aacaggcgct tatctttaca attacaggca
      601 gaaaacaacc gaaagcgaga aagaattcag aggggatatt gattttgaac ataattttaa
      661 tgataagagt aaactggaag ccggttttca gctccggata gaccgcacgg aagaagatta
      721 tgaatattta aatttcgata cgctttacac tagctggatc ttagatttag gtcaaagcaa
      781 tcagtacgat ttctctcatg atatttacgc tctgtatgga acgtatgcca ctgaaattaa
      841 aaaacttggc atcaaagcgg gcctgagggc tgaatacacc gaccggaacc tgcaccaggt
      901 cacgggaggt ggcgattacc cttatgagaa atttgatctt tacccaagcc tttacctgac
      961 ctataaactg ccttataacc aacagatgca gctttcttac agtaaacggg tccagcggcc
     1021 cagggggcat atgctgaacc cttatacgat gttttcagac gcattcagca gttttgccgg
     1081 caatccgcag cttgagcctg aattttcaca caacatggag ctgaattacc agaaatattt
     1141 cgggtactca tttcttacaa ttgaaaccta ttacaggctg accgacaata agatgaccag
     1201 ggttcagaaa ttaaatgatg aaggggtgat gatgataacg atggaaaata tcaataatga
     1261 cagatctttg ggtattgaag cctccggcaa tatcaaagcc aatacctggc tgaccatcaa
     1321 tccgatagct tctctttacg actatgcttt gaatcaaacg gaaaacggtg aaatagagac
     1381 ccgcaggagc accaactgga tggccagcct tgaactggcc gccaatctga aaacagggac
     1441 taaaatcagg cttaacggca actatgacag cccttccata actgtcgacg gcacccggaa
     1501 aggaaccttt tataccggtt tctctgcgcg ccaggatctg cttaataata acctgtcgat
     1561 cacacttaac atcagggata tatttgactc aaggcacatg aaagagacca gtgaaggcga
     1621 aaactattat ataaccggcg aaaactggcg taaagcgcct gttttcaata tcgccctgag
     1681 ttataaattc aacaattaca accgcaaaag atctgccgac agtttcgacg gtgactacga
     1741 tgtgatcaat atgaattctt atttctaaaa aaagaatatg atgaaaaaaa acattcttat
     1801 actggctgcg gttctgtttt gcctggccct cagcgtgaac gcgcagaagg tagcttacta
     1861 cgaatcgaaa tggtatccgg gtgtcgtgaa agataatgag gccgatctgt taaacctgat
     1921 gcagtataat gaaaagagcg ggatgctctt tctcgtttca aatgatgatt caaggttata
     1981 tatcacgctg gtggcaaccg ataaagcagc catccaaaag atcatgcgtt ttggcctgac
     2041 cacctggatc gaccctgaag cgaagggcaa gaaaagatat gccatcgaat ttccggttag
     2101 ttcagtcaaa ctgcctgcgc ctgcggccga tccggggcgt ggcgaccgta aagaagcccg
     2161 tatggccatg atggcggcta aaaacggttt aatgatcctt cagggttttg cagaaaagaa
     2221 tgagagcctg gaaattgatc cccgtatgga tgaaaatttc aatggccggt ttgaaatgat
     2281 ggaaggggga aaactctctg ttagtttaat ccttcccctc gaccggatgg gattatccat
     2341 tgccggtgca gccggtcatc ctgtcagtgt tgcttttgaa acagggtaca tggatgtgac
     2401 cggagcgatg ccagccggag gtggtcagcc gggaggtggc ggaatgcatg gcggaggcat
     2461 gtacggcggt gggatgcccg gaggaggacc gcctcccggt gcaggcggcc aggcaggcag
     2521 cgacacccag gatcgtcccg atattggcca actggcctct ccgacccggc tacggatcaa
     2581 aacagtaaag cttgcaccaa aaccttagtt cttaagactc taatcattaa ccgacggctt
     2641 tagtcgtcgg aagacccaca aaaaagaggc tgcctgatga acaagcagcc tcttcacatt
     2701 ccggatcaga tgatgatcct aaatcaattc attaccgggt gaccagcatc tttctggtaa
     2761 aggtctggtt atcaccctgg agcacaaccg tataaactcc cttaccagga ttgttcaggt
     2821 cgacagggat ttgtgcctta ccattcaccc ggatgttatt ccgctcatat actttgaccc
     2881 cgaggtcgct gtatacttta atggagaaca catcctcgcc gttaatggca atattggcaa
     2941 cgaacttacc atcgttgggg atcgggaaga tggagaatcc ggttccgttc atttcatcca
     3001 taccgacaaa gatcac
//
LOCUS       DAOPNH010000345         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_41061_length_2951_cov_4.816989, whole genome shotgun sequence.
ACCESSION   DAOPNH010000345 DAOPNH010000000
VERSION     DAOPNH010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41061_length_2951_cov_4.816989"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKZ74_13720"
     CDS             complement(<1..552)
                     /locus_tag="PKZ74_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93859.1"
                     /translation="MKQMHKALILFVFICLNSFAQEKIVVYQADAGCTPENKTIDISH
                     MKADLRIDPYDTLVEGEVEFTFRFINASADSVVFWAPDIEFSDVSIPGQKINYSKSGD
                     NLVVHNLESINYVKDDDYIIKMKYTSRPKYDLFFIGWNEPAVRDNKQIWAHRPFHWLP
                     YYSDRLTTDMRITFDGNYKVMTNG"
     gene            648..1994
                     /locus_tag="PKZ74_13725"
     CDS             648..1994
                     /locus_tag="PKZ74_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93860.1"
                     /translation="MKNSIYTFFFGAFFMSMTSNAQVNTMNLPGHEPSITNDQVRAFG
                     EASFESKSKVVVLDFEGIGNLDEITQFYNGGASSAGFSGKNYGISFGKSALALVDAQH
                     GGTGNFNTRPMPNTVMFFLKGNTTVINVHQGFKGPFAFDYTSSVLGTVAFYTEPDGGG
                     TLISTEQFQPLATGSKGIENGYFDNWKNYSASFSGTAKSVVITCAADQCAFDNILIGD
                     ETYKNSLPGGGPVVTGSKTGDTGGAFQAASTGSYYFPGTKGKLFVAGYSGLDISAGTR
                     KSKYNGEIDDGSKYSLFNIYFDPKAGYFIIDNLVAGLFIDLNYYSSKSKDEFGYSNKG
                     TTLEIGPFARYYFPICDKLVPYGEAQIGVGVDNSSSRSSSSSEWYKSKSSVFAYQLGG
                     GATYFFNSNVGADAFIGFSHYSNKYKDSESGERSPGSKYIYNELVLQLGIVIVLGY"
     gene            complement(2000..>2951)
                     /locus_tag="PKZ74_13730"
     CDS             complement(2000..>2951)
                     /locus_tag="PKZ74_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM93861.1"
                     /translation="MLQSERVKLLHMEEELHKRVIGQDEAIGALADAIRRSRAGLQDA
                     KRPVGSFIFLGTTGVGKTELAKALAEYLFNDENAMVRIDMSEYQERHTVSRLVGAPPG
                     YVGYEESGQLTEAVRRKPYSVILLDEIEKAHQDVFNILLQVLDDGRLTDNKGRIVNFK
                     NTIIIMTSNLGSQVIQERFAGLTVSNKELIVSQTREEVFNLLRLTIRPEFLNRIDDII
                     MFKPLNENEIRKVVLLQFNQLQAKLAKNNMDIRMTDGAADWVARQGFDPQYGARPVKR
                     IIQRTILNELSKMILSAEITSEKPVLIDCSDDRIIFKNVP"
BASE COUNT          798 a          677 c          657 g          819 t
ORIGIN      
        1 cccattggtc ataaccttgt aatttccatc aaaagtgatt ctcatatcgg ttgttagcct
       61 gtcgctataa tacggtaacc agtgaaaagg acggtgcgcc cagatctgct tgttatccct
      121 gaccgccggc tcattccagc cgataaaaaa aagatcgtat tttggacggg aagtatattt
      181 catcttaata atatagtcat catccttaac atagttaatt gattctaaat tatgtacgac
      241 taaattatcg cctgattttg aatagtttat cttttgacct ggaatggaca catcgctgaa
      301 ttcaatatca ggggcccaga aaacaacgga atcggcagaa gcgttaataa agcggaatgt
      361 gaactccacc tccccttcaa ccagcgtatc ataaggatcg atcctcaggt cggctttcat
      421 atggctgatg tcaatggttt tattttcagg ggtgcatccg gcatcagcct ggtatacaac
      481 gatcttctcc tgagcgaaac tgttcaggca aataaaaaca aataagatca gggccttatg
      541 catttgtttc atagggtgtt aatttttccc gaaaataaaa aaaaatgcat tggttttata
      601 ggattgtatt tatttttgat gtagcaatta atctaatctc agacaatatg aaaaattcca
      661 tctacacttt cttttttggt gcatttttta tgtcgatgac atcaaatgca caggttaata
      721 ccatgaatct gccgggacat gagccatcta taacaaacga ccaggtgaga gcattcggcg
      781 aagccagctt tgaaagcaaa tcgaaggtcg ttgtcctcga ttttgagggg atcggaaacc
      841 tggatgagat cacacagttt tataacggcg gagccagttc agcgggattc tcagggaaga
      901 attacggcat cagtttcgga aaatcggctc tggcactggt cgatgcgcag cacggcggca
      961 cgggcaactt taataccaga ccgatgccga atacggtcat gttcttcctg aaaggcaata
     1021 caaccgtgat caatgtacac cagggattta aaggaccgtt cgcatttgat tacacatctt
     1081 ctgtcctggg gactgttgct ttctacacag aacctgacgg aggcggtacg ctgatctcaa
     1141 ccgagcagtt tcaacccctg gccaccgggt ctaaagggat agaaaacggg tatttcgaca
     1201 actggaagaa ctattcggct tcattttcag gaacagccaa atcggtcgtg atcacatgtg
     1261 ctgccgatca atgcgcattc gacaatatcc tgatcgggga tgaaacatat aaaaattctt
     1321 tacccggagg agggccggta gttacaggat ccaaaaccgg cgataccggc ggcgcttttc
     1381 aggcagcatc gacgggaagc tattatttcc ccgggacaaa aggcaagctc ttcgtggccg
     1441 gttacagcgg tctggatatt agcgcaggca ccagaaaatc aaaatataac ggggaaatag
     1501 atgatggctc gaaatattcc ctcttcaata tttatttcga tccaaaagcc ggatatttta
     1561 ttatcgataa cctggtggcc gggcttttta tcgatctgaa ctattacagt tcgaagtcaa
     1621 aagacgaatt tggttatagt aataagggaa caacactgga aataggacct tttgccaggt
     1681 attattttcc gatttgtgac aagctggtcc cttacggaga agcacagatc ggtgtgggtg
     1741 tagataatag cagttcgcgc agttcctcct cttccgaatg gtacaaatcg aaatcctccg
     1801 ttttcgctta ccagcttggc ggcggggcca cctatttctt taactcaaac gtgggggcag
     1861 atgcatttat agggttcagc cattattcta ataaatacaa agattcggaa tccggggaaa
     1921 gaagcccggg cagcaaatat atttataatg aactggtact gcaactgggt attgtcatcg
     1981 tgcttggata ttaatccggt cagggcacat ttttgaaaat tatccggtca tcggaacaat
     2041 cgatcaatac cggtttttcg gatgttattt cggcactgag gatcattttt gacaattcat
     2101 tcaggattgt tcgctgaatg atccgtttca ccggccttgc accgtattgc ggatcaaatc
     2161 cctgcctggc cacccaatcc gctgcaccat cggtcatcct tatatccata ttgttttttg
     2221 ccagcttagc ctgcaactgg ttgaactgca acaggacaac cttacgtatt tcattctcat
     2281 tcaatggttt aaacataatg atatcgtcga tccggttaag gaactcaggt cggatcgtca
     2341 agcgaagaag gttgaatacc tcttcacggg tctggcttac aattagttct ttatttgaca
     2401 cggtcaaacc ggcaaaacgt tcctggatca cttgtgagcc tagatttgaa gtcatgatta
     2461 tgatagtgtt tttaaaattc actatccggc ctttgttatc agtcagcctt ccgtcgtcaa
     2521 gaacctgcag caggatatta aacacgtcct ggtgcgcttt ttcaatttca tcaagaagaa
     2581 tgacagaata aggcttgcgg cgaaccgctt cggttagttg cccgctctct tcatagccca
     2641 cataccccgg gggtgccccg acaagcctgg acacagtatg ccgttcctgg tattccgaca
     2701 tgtctatcct taccatcgca ttttcatcgt tgaaaaggta ttcggccagc gccttggcaa
     2761 gttctgtttt acccactccc gtagtaccca ggaagataaa ggaaccgaca ggccttttgg
     2821 catcctgcaa accggccctg gaacggcgga tcgcatcggc cagagctccg atcgcttcat
     2881 cctgccctat cacgcgcttg tgtaactctt cttccatgtg cagcagtttc acgcgctcac
     2941 tctgaagcat c
//
LOCUS       DAOPNH010000346         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_42702_length_2871_cov_3.871449, whole genome shotgun sequence.
ACCESSION   DAOPNH010000346 DAOPNH010000000
VERSION     DAOPNH010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42702_length_2871_cov_3.871449"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKZ74_13735"
     CDS             complement(<1..530)
                     /locus_tag="PKZ74_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93862.1"
                     /translation="MKSLIFNLFLILLAVSSAGQIAPDKYFVQFTDKNNSPYSIDNPS
                     EFLSERAIERRASQGISVDIYDLPVNPSYLSGVAGTGATLLNPTKWLNGVTVFTDNPA
                     VLEAIDALPYVAGITGYPQEKSQPGRIQYDKEKPFFYHEMYNQAPGNSYKSKAQTGSF
                     DYGMGFNQIQMLNGDV"
     gene            630..1316
                     /gene="trmD"
                     /locus_tag="PKZ74_13740"
     CDS             630..1316
                     /gene="trmD"
                     /locus_tag="PKZ74_13740"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPM93863.1"
                     /translation="MRIDIITLFPDIFRGPFDFSIIKRAIDKNLVTIGLHDLREYATN
                     KYKSVDDYPFGGGAGMVMAIEPIDRCISLLKQQRHYDEIIFLTPDGELLDQPLGNRLS
                     MCENIILLCGHYKGIDERVRQHLVTREISIGDYVLSGGELAAAVLTDCLVRLLPGVLG
                     DETSALSDSFQDGLISPPVYTRPAVYKGWAVPEILLSGHEAKIKEWRLDQAVERTKIR
                     RPGLLKDFSE"
     gene            1425..1778
                     /gene="rplS"
                     /locus_tag="PKZ74_13745"
     CDS             1425..1778
                     /gene="rplS"
                     /locus_tag="PKZ74_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPM93864.1"
                     /translation="MSKNELMQYVDTLTTVKEFPDFKAGDTITVTYKIREGNKERLQK
                     YQGVVLQRVGSGPTETFTVRKISANIGVERIFPISSPFIDEIQVNKRGRVRRARIFYL
                     RDLKGKKARIKEARF"
     gene            1866..2084
                     /gene="yidD"
                     /locus_tag="PKZ74_13750"
                     /pseudo
     CDS             1866..2084
                     /gene="yidD"
                     /locus_tag="PKZ74_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            2255..2695
                     /locus_tag="PKZ74_13755"
     CDS             2255..2695
                     /locus_tag="PKZ74_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPM93865.1"
                     /translation="MSLQFKPEIVTVCRKVHFNATHRLHNPNWTDEKNEKVFGKCNNE
                     NYHGHNYDLVVRVTGPVDPETGYVIDLKVLNDLVRQHVIERYDHKNLYLDVPDFKDLN
                     PSAEYIAIKIWQIIRERIKPELRISIQLFETERNFVEYNGPVNQ"
BASE COUNT          838 a          580 c          653 g          800 t
ORIGIN      
        1 aaaacatctc cgttcagcat ctggatctgg ttaaatccca tcccataatc aaatgaaccg
       61 gtttgggctt tacttttata ggagtttccg ggcgcctggt tatacatctc atgataaaag
      121 aacggcttct ctttatcgta ctgaatcctg ccaggctgag acttttcctg tggataaccg
      181 gtgatgccag cgacatatgg caaggcatcg attgcttcca gaacagccgg attatcggta
      241 aaaacagtaa ccccattcag ccatttggtt ggatttaaca gggtggcacc tgtaccggca
      301 acacccgaaa ggtaggaagg atttaccgga aggtcataaa tatctacgga gattccctgg
      361 ctggcccggc gttcaatggc ccgttcagac agaaattctg aaggattatc gattgaatag
      421 ggggaattat ttttatcggt gaattggacg aaatatttgt cgggtgcaat ctggccggca
      481 gatgaaactg caagaagaat cagaaataag ttgaatatca gtgatttcat atttgtgaat
      541 ttttgcaaaa atagtaaata tcaggtgatt gaatcatgat cgtaacaatc cggcggggga
      601 tttgtttaaa aaacatatct ttgaaaatta tgaggattga tatcattacc ttatttccgg
      661 atattttcag ggggcctttt gatttttcaa ttatcaaaag agcaattgat aagaacctgg
      721 tgaccattgg gcttcatgac ctccgggaat acgcaaccaa caagtataag agcgtggatg
      781 attatccgtt cggaggtgga gcaggaatgg tgatggcgat tgaaccgatc gacaggtgca
      841 tcagcttgtt gaaacagcaa aggcattatg acgaaatcat ttttctgaca cccgatgggg
      901 aattgctgga tcaaccactg ggcaaccgtc tctccatgtg tgagaatatt attttattgt
      961 gcggacatta taaaggtatt gatgaaagag tccgtcagca ccttgttaca cgggaaatat
     1021 ccatcgggga ttacgtcctt tcaggtggcg agctggcagc ggccgtgctg accgattgcc
     1081 tggtccgcct gttacccgga gttttaggtg atgaaacttc cgccctgtcc gattcattcc
     1141 aggatggttt aatttctccg ccggtttata ctcgccctgc tgtatacaaa ggctgggctg
     1201 tcccggaaat cctgttatcc ggacatgagg ccaaaataaa ggaatggcgg ctggatcagg
     1261 ctgttgaaag aactaagatc aggcgtccgg gcttattaaa ggatttttcc gaataaaatc
     1321 gatcccactt cttaaattat tttgagaata agcctttgat tactggtaaa tagttgtata
     1381 tttgcacact tttttgaaag aaccgcttaa aagatttgac agacatgagc aagaacgaat
     1441 taatgcaata tgtggatacc ttaacgactg ttaaggaatt tcccgatttc aaagcaggtg
     1501 atacgatcac cgttacatat aagatccgcg aaggtaataa ggaaagattg cagaagtatc
     1561 agggtgttgt attacagcgc gttggatcag gccctaccga aacgtttacg gtacgaaaaa
     1621 tttcagctaa cattggcgtc gaaaggatat ttccgatctc ctctcctttt atagacgaaa
     1681 tccaggtcaa taagaggggc cgtgtaagaa gggccagaat tttctacctc agggatctta
     1741 aaggcaagaa agcccgtatt aaagaagcca ggttctaaaa cttcggatat agttattttg
     1801 acaagatatt cagagggcgc tttccaaaag agcgccctct tgctttttgt tattatcttt
     1861 gaatcatgcg acgctatggt acatggccct ttatcggact gatctatgtt tacaggtata
     1921 ttatttcccc actattaccc aggggctgca ggcattatcc aagttgttcg gaatatgcca
     1981 tcacggcttt aaaacagcac ggtttactca aaggaggact tctggccgca aaccgtattt
     2041 cccgttgcca gccctggggt accagcggaa ttgatccggt tcctcgcatt attgcaggaa
     2101 aaataaaata ttccaggttc cgtcagcgac aaaaaatgaa aagattccca acctgtaatc
     2161 gtctcaaacg gccataattc ttttgaattt gctatttttg tataaccggt gacctggtca
     2221 tcaattgaat ataatcattg aaaattccac atccatgagc ttgcaattta aacctgagat
     2281 cgttacggtg tgccgcaagg tccatttcaa tgccacgcac cgtttgcata atcctaactg
     2341 gacggatgaa aagaatgaga aggtatttgg aaaatgcaac aatgaaaact accacgggca
     2401 taattatgac ctggtggttc gagtcaccgg tccggtagat ccggaaacag gctatgtcat
     2461 cgatctgaag gttctcaatg atctggtcag gcagcatgtc atagaaaggt atgaccataa
     2521 aaacctctat ctcgacgttc ctgattttaa agatctgaac ccttcagcgg aatatattgc
     2581 catcaagatc tggcagataa tccgggaaag gatcaaaccg gagctgcgaa tctccattca
     2641 gttatttgaa acggaacgaa actttgtcga gtacaatggt cctgttaatc aatgatataa
     2701 ttattaacta gaacaatctg aagggattaa tggaacatca ccatgaacat gcagcgcctc
     2761 tgagtgctga aaatctgctc tctgtgcttc agcatacttt aatggtcacg ttttttgtgc
     2821 tcattgtcat gctgatgatc gagtatatta ccgtacagac aaaaggaaga t
//
LOCUS       DAOPNH010000347         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_43343_length_2841_cov_4.305456, whole genome shotgun sequence.
ACCESSION   DAOPNH010000347 DAOPNH010000000
VERSION     DAOPNH010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43343_length_2841_cov_4.305456"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PKZ74_13760"
     CDS             <1..1309
                     /locus_tag="PKZ74_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM93866.1"
                     /translation="VWAVTTLMTVLAALSYGELASMLPHVGGIYVYLKEAYSPLFGFL
                     YGWTFFLVIQCGTIAAVAMAFAKFTGVLLPVISEQNILLDLGLFKFNTTQLLAIMMIL
                     FLTWINTRGIKEGKRVQNIFTYSKVFILLAFIVIGIFVAKATQLNVFHSPGFWEASKI
                     VDNQQVPVSGFSLIIAIGLAMVGSIFAADAWYNITYTSDEVINPKKTIARSLFLGTLT
                     VCLIYFLVNVVYIIALPVRGIPDGATVMERGIQFATADRVATAAIYGIFGKNTELLMA
                     VVVVISTFGCNNGIILSGARVTYAMAKDRLFFKKTGELSKTGVPAFGLWIQAIWSTLL
                     CLTGTYSQLLDYVVFAALLFYILVIFAVFMLRIKHPEWERPYKAFGFPVFPIIYIAAC
                     LLIIIILLIYKPLFTWPGLGIVISGIPVYYLWKRKSHKGPVTI"
     gene            complement(1310..>2841)
                     /locus_tag="PKZ74_13765"
     CDS             complement(1310..>2841)
                     /locus_tag="PKZ74_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM93867.1"
                     /translation="EGYGMTEASPMITFPRPGKVVIGTAGQALPGLKVEIREGEVVAK
                     GPNIMQGYYKRPEETAEVLRDGWLYTGDLGEIDSKGYLRITGRKKEIIVLPNGKNVNP
                     VELEQKLEKEYACVKEAGVLVQNDILHAVILPDFTALANAGIKDADLYFRTDVFPAFN
                     EELTSYKRIMQFTLVKEELPRTRLGKIQRFKLDELAVGKTKEKRQSAEEDSSEEYLAV
                     KTFLESQVDMTISPEDHLEFDIALDSLGKLSLIDYIEKTFGVKIEEEHLPKFPQVKSL
                     VEYIKINKKWQKEDVTSWASALKEKVQVKLPRTWPTLPMIKNSAKGFFAVYSRLKSRG
                     TENIPEGPCIIAPNHESFFDGLYLVAALKMKTMKKSYFYAKKTHVNNWFLRFMANRNN
                     VIVMDVNKDLKDSIQKLAEVLRKGKNVIIFPEGTRTKTGKMGDFKKTFAILSKELNIP
                     VVPVAISGAYKAMPYRAIFPRPLSKISICFAEPVYPDSFSSDELTAIVRDRISNMLNC
                     R"
BASE COUNT          673 a          692 c          604 g          872 t
ORIGIN      
        1 ggtctgggct gtcaccaccc tgatgacagt actggctgcc ctgagctacg gcgaactggc
       61 cagcatgctg ccacatgtcg gcggcattta tgtttacctg aaagaggcct attctccatt
      121 attcggtttt ctgtacggat ggaccttttt cctggtcatt cagtgtggta ccattgccgc
      181 tgtcgccatg gcttttgcaa aatttacagg agtgctgctg ccggttatct cagaacaaaa
      241 cattctcctg gatctgggat tattcaaatt caacactacc caattgctcg ccattatgat
      301 gatccttttc ctgacatgga tcaatacccg gggaatcaaa gaagggaaaa gagtgcagaa
      361 tattttcacc tattccaaag tatttatcct gctcgccttt atcgtcatcg gaattttcgt
      421 ggccaaggct acacaattga atgtttttca ttcacccgga ttctgggaag cttccaaaat
      481 cgttgataac cagcaggtcc cggtatcggg tttctcactg atcatcgcga tcggattggc
      541 gatggttggc tctatcttcg ccgcagatgc ctggtacaat atcacgtata cttcggatga
      601 ggtcatcaat cccaaaaaga caatcgcccg aagtcttttc ctgggaactt taacggtctg
      661 ccttatctat tttcttgtta atgtggtata tatcatcgca ttgccggttc gagggatacc
      721 cgatggtgct accgttatgg aaagaggaat ccagtttgct acagccgaca gggttgcgac
      781 cgccgctatt tacggtattt tcggaaagaa tacggaattg ctgatggctg ttgtagttgt
      841 tatctccacc tttggatgta ataatggcat tattctttcc ggagccaggg ttacttatgc
      901 catggccaaa gaccggcttt ttttcaaaaa aacaggtgaa ctgagcaaaa caggtgtccc
      961 ggctttcgga ttatggatcc aggccatctg gtcaactctc ttatgcctga cgggtactta
     1021 cagtcaatta cttgattatg ttgtgtttgc agcattactg ttctatatcc tggtcatatt
     1081 tgccgtcttc atgctcagaa taaaacaccc ggaatgggaa cgcccataca aagcattcgg
     1141 atttcctgtc ttcccgataa tttatatagc agcatgcctg ctgatcatca ttattcttct
     1201 catttacaaa cccctgttta cctggccggg attgggaatt gtcatttcag gaatacctgt
     1261 ttactattta tggaaaagga aaagccacaa aggcccggtt accatctgat caccggcagt
     1321 tgagcatgtt gctgatcctg tccctgacta tggcggtaag ttcatcactg gaaaaactat
     1381 ccggataaac cggttccgca aaacaaatac ttatcttact caaaggacgc ggaaaaattg
     1441 ccctgtaagg catggcttta taagcgccgc taatggcaac gggcaccacc gggatattca
     1501 gttccttgct gaggatggcg aaagtcttct tgaaatctcc catcttgccg gtctttgtcc
     1561 gggtcccttc cgggaagatg atgacgttct tgccctttct aagtacctcg gccagtttct
     1621 ggatggaatc ttttaaatct ttattcacat ccatgactat gacattgttc cgatttgcca
     1681 tgaaccttag gaaccagtta ttaacatgcg ttttcttagc atagaaataa ctctttttca
     1741 tggtcttcat ctttaaggcc gctacaaggt ataggccatc gaaaaaactc tcatgattag
     1801 gggcaataat gcaaggtcct tccggaatgt tttcggttcc cctgcttttc agcctggaat
     1861 aaacagcaaa aaatcctttt gccgagtttt ttatcatagg tagggttggc caggttctgg
     1921 gcagtttgac ctgcactttc tccttcaatg ctgaagccca gctggttaca tcttcttttt
     1981 gccatttctt atttatttta atatattcaa cgagtgattt gacttgtggg aactttggca
     2041 ggtgttcttc ttcaatcttt acgccgaatg ttttttcgat atagtcgata agactcaact
     2101 tacccagcga atcaagtgca atgtcgaatt caaggtgatc ttccggggaa atcgtcatat
     2161 ccacctggct ttccaggaag gttttcacgg ccaggtactc ctcggaagaa tcctcttcgg
     2221 ccgattgtct tttctctttc gtttttccta ctgcaagttc atccagctta aaccgctgaa
     2281 tttttccaag acgggtccgg ggtagctctt ccttgaccag cgtaaactgc atgatccttt
     2341 tatatgaagt aagttcttca ttaaaggcag gaaaaacatc ggttctgaaa tacaggtctg
     2401 catcttttat acctgcattg gccagcgcag tgaaatcggg cagaattacc gcatgaagga
     2461 tgtcattctg aacaagtacg ccagcttcct taacacaggc atactctttt tccagttttt
     2521 gctcaagttc gacaggatta acattcttcc cgttcggaag cacgattatt tctttctttc
     2581 tgcctgtgat cctgagatat ccttttgaat cgatctcccc caggtcaccc gtataaagcc
     2641 agccatctct cagtacctcg gccgtttcct ccggcctctt ataatagccc tgcatgatat
     2701 ttgggccttt ggcgacaact tccccttccc ggatctcaac tttcagtccc ggtaacgcct
     2761 gtcctgccgt gccaatcacg accttgcccg gcctgggaaa ggtgatcatg ggtgatgcct
     2821 cggtcattcc atagccctcc a
//
LOCUS       DAOPNH010000348         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_43475_length_2835_cov_6.960791, whole genome shotgun sequence.
ACCESSION   DAOPNH010000348 DAOPNH010000000
VERSION     DAOPNH010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43475_length_2835_cov_6.960791"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKZ74_13770"
     CDS             <1..984
                     /locus_tag="PKZ74_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPM93868.1"
                     /translation="LATAKGAAGVIIIARKDSDFRDFLKNRDQYETEPNISYPEPGEE
                     DVTKKEVFSAFVDLKTAAMLVGEKPAVLSAAMAEMESSLATSAGRFAGRVEIDASSDC
                     VALPTANIVAIVEGSEKKHEAVVVVAHYDHLGEFAGDIYYGADDNASGTAAVMELAEA
                     FSRAAAEGVRPKRTVVFLAVSAEELGLYGSRHYTDHPLIPLDSTVACINIDMIGRSSE
                     KQVTSDYISGYVYRSPELYALTQHAHRLAAPGLLDRMELRQSVRGGSDHYYFAKHGIP
                     SVFYFTGFHEDYHEPTDTPDKILYTRMEQIVRAIFAVTWKLANGEEKMDSE"
     gene            1132..1205
                     /locus_tag="PKZ74_13775"
     tRNA            1132..1205
                     /locus_tag="PKZ74_13775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1166..1168,aa:Ala,seq:ggc)
     gene            1316..2212
                     /locus_tag="PKZ74_13780"
     CDS             1316..2212
                     /locus_tag="PKZ74_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93869.1"
                     /translation="MKTKALYECIDFLTKWKTIKYDDHIHETYRDAIYGEKYVEPFRR
                     ELNKNLVFLNQTKQNKVIKFYITELIRQIDSEFILSKYKTQDSEESGTLEIKDEAYLK
                     EKEEKYSMADAITYLNMILFFELQMICRSYNIPVKKIIDEANIDLDKYYFIPALKKFK
                     GFEESFTLSIRPRFTAEYIPQIFEFLKDFFDKRDQPEFLTILETGEDAYRPLLFIDKG
                     SRLADVFKQLFSVDIIRGCQKKELEAWICKNFRYRSGKTIKIFTKRYVSDVISSNQVR
                     ITKPILDLKLDKTTGQYLITKL"
     gene            2315..2653
                     /locus_tag="PKZ74_13785"
     CDS             2315..2653
                     /locus_tag="PKZ74_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM93870.1"
                     /translation="MTEITFEKLPQAVTQIYSKLESIERLLHAQNNLSHPAEDKLLTI
                     REAAEVIHLSVPTIYGLVQRQDIPVCKKGKRLYFSRQELNDWIKSGRKKTVAEITAEA
                     GNYLQIRNKR"
BASE COUNT          841 a          570 c          688 g          736 t
ORIGIN      
        1 ctggctactg cgaagggcgc cgcgggggtc atcataatag cccggaagga tagcgatttc
       61 cgcgatttcc tgaagaaccg ggatcagtat gaaacggagc cgaacatctc ttacccggaa
      121 ccgggggaag aagacgttac aaagaaggag gtcttttcgg catttgtcga tctgaaaacc
      181 gcggccatgc tggtagggga gaagcctgcc gttttatcgg cggcgatggc cgagatggaa
      241 tcgtcgctgg ctacatcggc aggacgcttt gccgggaggg tggagatcga tgcgtcgagc
      301 gattgtgtgg cattgccgac ggccaatatt gtcgccatcg tggaaggatc ggagaagaag
      361 cacgaagccg tggtggtggt ggcccattac gatcacctgg gcgagtttgc gggcgatatc
      421 tattacgggg ccgatgacaa tgcttcgggt acggcggctg tgatggagct ggcggaagcc
      481 ttttcccggg ctgctgctga aggcgttcgc ccgaagcgga ccgtcgtatt cctggcggtg
      541 tcggccgaag agctgggcct ttacggatcg cgccattata cggatcatcc gctgattccg
      601 ctcgacagca cggtcgcctg catcaatatc gacatgatcg ggcggtcgtc ggagaagcaa
      661 gttacgtccg attacatttc cggttatgtt taccgctccc cggagctata tgcccttacc
      721 cagcatgccc accggctggc agcgccgggt ttgctcgacc ggatggagct gcgccagtcg
      781 gtgcgcggcg gttcggatca ttactatttt gcaaagcacg ggatcccgtc tgttttttac
      841 ttcaccgggt tccacgaaga ttaccatgaa cccaccgaca cccctgacaa gattctttac
      901 acccgcatgg agcagatcgt ccgtgccatc tttgccgtca cctggaagct ggcgaacggc
      961 gaagagaaga tggatagtga atagtgatta gggaataacg aatagtgatt agggaaaaat
     1021 ggttagtact ttaaaaaaaa agtttatttt tgcaccgcgt tttaggattt caggttttga
     1081 atttgtttgg aatttggaat ttggagcttg tgatttggaa ttttaaaatt tggggtatta
     1141 gctcagttgg ctagagtgct tgactggcag tcaagaggtc atcggttcga ctccgatata
     1201 ctccacagaa aatcaggcgg ttacgaaagc aaaaagtagc cgcctgattt atttactaac
     1261 attttcacta acatttgatg ccgggctggc atctgacttt accttaacat atcgcatgaa
     1321 aacaaaagca ttgtacgaat gcatagattt tttgaccaaa tggaagacaa tcaaatatga
     1381 tgatcacata catgaaacat atagggatgc tatatatggt gaaaaatatg ttgaaccttt
     1441 tagaagggaa ctgaataaaa accttgtatt cctgaatcag accaagcaaa acaaagtaat
     1501 taaattttac atcacggaat tgataaggca aatcgattct gaatttatat taagcaagta
     1561 taaaactcag gatagtgagg aaagtggtac attggaaatt aaagatgaag catatctcaa
     1621 ggaaaaagaa gagaaatatt caatggccga tgcaatcaca tacctgaata tgattctatt
     1681 ctttgagctt cagatgattt gcagatctta taatattccg gttaaaaaaa ttatcgacga
     1741 ggcaaatatc gatctcgata agtattattt tattccagcc cttaagaagt ttaaaggatt
     1801 tgaggaatct tttacgttaa gtataagacc aaggtttact gctgaatata tcccccagat
     1861 atttgagttt ctaaaggatt tctttgataa gcgtgatcaa ccggaatttc tcactatatt
     1921 ggagacagga gaagatgcct atagacctct tttattcatc gataagggtt cgcgattggc
     1981 tgatgtattt aaacagcttt tcagcgttga tattataaga ggatgccaga aaaaagagct
     2041 tgaagcctgg atttgtaaaa actttcgtta ccgcagcggt aagactatca aaatcttcac
     2101 taaaagatat gtctccgatg ttatctcatc aaatcaggta agaatcacaa aacctattct
     2161 tgatttaaaa ctggataaaa caactggaca gtatctgatt acaaagctat aaaatcttat
     2221 acggatttta tacgtgtggg tgcccaccgt attgtaaccc cgctcaagac catagtacca
     2281 ttgcagcaag ttaaacaatt taaacttgta agcaatgaca gaaattacct tcgaaaaatt
     2341 accccaggcc gtcacccaga tctatagcaa gctggaaagt atcgaacggc tgttacatgc
     2401 tcagaacaat ctatcccatc ctgcagagga taaactgctg accattcggg aagctgctga
     2461 agtcattcat ctttcagttc ctacaattta cggactggtt cagcgccagg atataccggt
     2521 ttgtaaaaaa ggcaagcgtc tttacttttc ccgacaggaa ttgaatgact ggattaaatc
     2581 aggaaggaag aagaccgtgg ctgagatcac tgctgaagcc gggaactatt tgcaaatcag
     2641 aaataagcga tagccggata tgaaagccgc gaaaaaacaa cctccaattg ttgaaatcca
     2701 gcatgtactg aattcctggt acgatctacg tttcaatgag atcaggggag tggttgaagg
     2761 ccggaagaag gatgaggagg attacaaaga gctgaatgaa aagatacttg agataaaact
     2821 ttcaatatac tatcc
//
LOCUS       DAOPNH010000349         2833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_43529_length_2833_cov_6.168107, whole genome shotgun sequence.
ACCESSION   DAOPNH010000349 DAOPNH010000000
VERSION     DAOPNH010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43529_length_2833_cov_6.168107"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKZ74_13790"
     CDS             <1..871
                     /locus_tag="PKZ74_13790"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93871.1"
                     /translation="VIWEQTVQVNLAGENSSNNWNSPNPGGLLGQAIIALDSTGGDFD
                     GNLYLLCSVERNNTSDPRDVMFSRSTDGGWTWSAPVRINDDLGNNAYQWFGTMSVAPD
                     GRIDVVWLDTRNNLGSVNSALYYSYSLDGGVTWTPNEQLSEIFNPHVGWPQQNKMGDY
                     FDMYSDEEGAHLAWAATFNNEQDVYYSVITPDYIGVDEKYGITGITLLQNYPNPFKGS
                     TTIRYNLTDNTQVALQIFTTDGREIATLVDGYEQAGSHQVSFDAVNLKNGVYLYKLTT
                     DKSSITKRFTLIN"
     gene            895..1758
                     /locus_tag="PKZ74_13795"
     CDS             895..1758
                     /locus_tag="PKZ74_13795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPM93872.1"
                     /translation="MNDLIRIAIAYDFDGTLAPGNMQKHSFIPKLGLDKDEFWRESNA
                     MSKEHDMDDILSYMQLMLQKARERNIPVTRRALLDHGRQIRFFSGVESYFEEINRYAG
                     SKNVVIDHHIISSGVRDIIKGTVIAPYFKNIFASGYVFDNKDVAIWPALAINYTNKTQ
                     YLFRINKGIDNSFDNSLINKYVEEERRPIPFSRMIYLGDGETDVPAMKMIKFKGGTAI
                     AVYDESKLQDHGKASAREICEDLIRQNRADFIAPADYSKSSKLFKILQHLINRMVEEE
                     RLLRLKNGDFA"
     gene            2049..>2833
                     /locus_tag="PKZ74_13800"
     CDS             2049..>2833
                     /locus_tag="PKZ74_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM93873.1"
                     /translation="MIPRQLEEIILAKLFQGKAVIVLGPRQVGKSTLLKLIQQKRDEK
                     VLFLNCDFNDIRKQLTEPGINDLRRLIGDHRLVFIDEAQRVMNIGMTMKLITDQMEGI
                     QLVVTGSSSLDLSNVINEPLTGRKFEYYLYPLSIKEIVDYYGFVETNRRLEQFMIYGT
                     YPDVLNNPGHEAEILSNLVSSYLFKDVLIFQDIRKPEFIEQLLEALALQVSSEVSFNE
                     LAQLLKTDPHTIQRYISLLEKAFIISRMGSARATGSLSSRVELV"
BASE COUNT          779 a          591 c          739 g          724 t
ORIGIN      
        1 cgtgatctgg gagcagacgg tacaggttaa cctggcaggg gagaacagca gcaacaactg
       61 gaattcgccg aatcccggag gtcttctggg ccaggccatc atagcgcttg attcgaccgg
      121 aggagacttc gatggtaatc tgtatttgtt gtgttcggtg gaacgcaata atacctcaga
      181 tccgcgtgat gtgatgttct cacgcagcac agatggtggc tggacctgga gtgctcccgt
      241 caggatcaat gatgacctgg gcaataacgc ttaccagtgg ttcggaacga tgtcggttgc
      301 cccggatggc cggattgacg tagtatggct cgatacccgc aataacctcg ggtcggtgaa
      361 ttcggcgctc tattattcct attcgctgga tggcggtgtc acctggacgc caaacgaaca
      421 attgtcggaa atattcaatc cccatgtcgg ctggccccag cagaacaaaa tgggtgacta
      481 ttttgatatg tattcggatg aagagggcgc tcacctggcc tgggccgcta cattcaacaa
      541 tgaacaggat gtctattatt cggttattac acccgattat attggagtgg atgagaaata
      601 cggcataacc gggatcactt tgctgcaaaa ctacccgaat ccgttcaaag ggtcgacaac
      661 cataaggtac aatttaacag acaatacgca ggttgcttta cagattttta caaccgacgg
      721 gcgggagatt gccacgctgg ttgacggcta tgagcaggcg gggtctcacc aggtaagctt
      781 tgatgccgtt aacctgaaaa acggggttta cctctataag ctgacgacgg ataaatcatc
      841 cattaccaaa agatttactt taattaatta gttataatta gttgaataac aattatgaat
      901 gatctgatcc ggattgccat agcctacgac ttcgacggca cgctggcgcc gggaaacatg
      961 cagaagcatt cctttattcc caaacttgga ctggataaag atgagttctg gcgggaatcc
     1021 aatgccatgt cgaaggagca cgatatggac gatattcttt cgtatatgca gcttatgttg
     1081 cagaaagccc gggagaggaa tataccggtg actcgccggg cgttgctgga tcatggccgg
     1141 cagataaggt ttttcagcgg tgtggaatct tattttgaag agatcaaccg gtatgccgga
     1201 tcgaagaatg tggtgatcga tcatcatatc atttcttcgg gggtaaggga cattatcaaa
     1261 gggaccgtga ttgcgccgta tttcaagaat attttcgcgt cgggttatgt gtttgataac
     1321 aaagatgttg cgatctggcc ggcgctggcg ataaactaca ccaacaagac ccagtattta
     1381 ttccggatca ataaggggat cgacaattct ttcgataatt cgttgataaa caaatatgta
     1441 gaagaagagc ggaggccgat cccgttttcc aggatgattt acctgggtga cggggaaacg
     1501 gatgttccgg ccatgaagat gatcaaattt aaaggcggga cggcgattgc agtatacgat
     1561 gaaagcaaac tccaggatca cgggaaagct tcagcgcggg agatctgtga ggacctgatc
     1621 cggcagaacc gggccgattt tattgcaccg gccgattatt cgaaaagttc gaaactgttt
     1681 aagattttgc agcatttgat caaccgcatg gtggaagaag agcggctgct ccggttaaaa
     1741 aacggggatt ttgcttgatg ctgtcgggta cttccgacgc tgacaggtca accttagtcg
     1801 gacgctgaca ggtcggcatc ctttcaatcc tgaacacgtc cgccggctgg cggacctgtc
     1861 agcgatggaa agaatttccg atccggtact ggttgcgcaa aaatttcatt tctaacccgg
     1921 cgcgccagat gatcaaatga gaagagtgga gggggtagta aagtctgaat cataactcat
     1981 gattattata atattcctga tctacgttct aaatttatta tctttgtatc gttaaaatat
     2041 gccgtgatat gattccaaga caacttgaag aaataatcct tgcaaagctt tttcagggaa
     2101 aagccgttat agtactgggt ccaaggcagg taggtaaatc cacgctgctt aaattgattc
     2161 agcaaaaaag ggatgagaag gtgctctttc tcaattgcga cttcaacgat atcagaaaac
     2221 aactgacaga gccaggcatt aacgatcttc gaaggttgat cggggaccat cggctggtct
     2281 ttatcgatga agcccaacga gttatgaata tcggaatgac aatgaaactg atcacagatc
     2341 agatggaagg catacaattg gtagtgacgg gctcttcgtc actggatttg tcaaatgtca
     2401 tcaatgaacc gctcaccggc agaaaattcg aatattacct ttatccgttg tcgattaagg
     2461 aaatagttga ttattacgga tttgttgaga ccaataggag gctggaacag tttatgatct
     2521 acggaaccta tcctgatgtt ttaaataacc cgggacatga agcagaaata ttaagtaacc
     2581 ttgtcagcag ttacttgttc aaagatgtgc ttattttcca ggatatccgg aagccagaat
     2641 tcatcgagca actacttgag gcgcttgcgc tacaggtttc gtcggaggtt tcattcaatg
     2701 agctggcgca gttactgaag acggatccac acactattca gaggtatatc agcctgcttg
     2761 aaaaagcctt tatcatttcc aggatgggca gcgcgcgggc gaccgggtcg ctgtcgagcc
     2821 gggtggagtt ggt
//
LOCUS       DAOPNH010000350         2725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_46032_length_2725_cov_5.462547, whole genome shotgun sequence.
ACCESSION   DAOPNH010000350 DAOPNH010000000
VERSION     DAOPNH010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46032_length_2725_cov_5.462547"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKZ74_13805"
     CDS             complement(<1..229)
                     /locus_tag="PKZ74_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPM93874.1"
                     /translation="MKLPKIKNIALVAHDKMKADLVEWVRWNKEVLAQHKLICTGTTG
                     RLIQDLLSKEKDSLPGQDFEVIRLKSGPLGGD"
     gene            complement(317..1675)
                     /gene="thrC"
                     /locus_tag="PKZ74_13810"
     CDS             complement(317..1675)
                     /gene="thrC"
                     /locus_tag="PKZ74_13810"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPM93875.1"
                     /translation="MLKYYSTNGKSPSISFREALLQGQAPDKGLYMPETIPQISPEEI
                     YTFINKSYPEIAFDVTWKFLEGEIPAEDLKKIVEEAYDYPVPLEPVYERKYILRLDQG
                     PTASFKDFAARMMGRLMNYYPARENKQLLILTATSGDTGSAIANAFYGLDNIKVLVLF
                     PEKEVTLRQRKQMTTLGKNVEVISVEGKFDDCQALVKEAFADPDLLHLNLSSANSINI
                     GRLIPQIVYYFYAFARLRVGNPDDDVIFSVPSGNFGDMMGGVLAMKMGLPVWRFIIAV
                     NENDEFPNYLGTGNYMKVVPSRNCISSAMNVGHPSNLARLVALYGGKMDEKGQMSAQA
                     DLHTMRKELFAVSITDEETRRTIIDTWNMHNVLLEPHGAVGWAGLMAYLNEEGKDMDK
                     DQLCISLETAHPAKFPEEIDKLLGIDPPLPPSLQGIESKTEVFGKMKNSYEEFRERLK
                     EE"
     gene            complement(1731..2435)
                     /locus_tag="PKZ74_13815"
     CDS             complement(1731..2435)
                     /locus_tag="PKZ74_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPM93876.1"
                     /translation="MQTELIREILEKHLSPNDRYTYGFADLKNLIDKTFGDFHYGIAI
                     GRRLDPGIVNGIMNGPTLEYYRHYRAINTELDASTQKIADDLKKEGINAICISPTVTT
                     EELDTVYAGNLTYPVSHKMLATRAGLGWIGKSDLFISKAFGPRLRLVSILIDTPLDTS
                     PEPVETSRCGNCRICMDRCPAGAINGKPWDVSVHRDEYFDAFMCREMCKELGEKTLKM
                     NVRVCGICVAVCPFGW"
BASE COUNT          622 a          688 c          627 g          788 t
ORIGIN      
        1 ggtcgccacc cagcggcccc gacttgagcc ggataacctc aaaatcctgc ccgggaagtg
       61 aatccttttc ttttgacagc aggtcctgga tcaatcttcc ggtcgtcccg gtgcagatca
      121 gcttgtgctg agccagtacc tctttattcc acctgaccca ttccaccagg tcggctttca
      181 tcttatcatg cgccaccagc gctatgttct tgatcttggg cagtttcatc gctcaaatat
      241 tagttgttcc ggagttccag gttccaatgt tcgtttgttc tattgttcta ttgttaataa
      301 attccctttt cccttttcac tcttccttta atcgttccct gaactcctca taagagttct
      361 tcatttttcc aaacacctcc gtctttgatt ctattccctg gaggctgggg ggaagcggcg
      421 gatcgatgcc caggagcttg tcgatctctt cggggaactt tgccggatgg gctgtttcca
      481 gcgatataca aagctggtcc ttgtccatat ctttcccttc ttcattcaga taggccatca
      541 atccggccca accgacggcg ccgtgcggtt caagaaggac attgtgcatg ttccaggtgt
      601 cgataatggt gcggcgtgtc tcttcatccg tgatactgac ggcaaacaat tcctttcgca
      661 tcgtatgaag atctgcctgt gcagacatct gccctttctc gtccattttt cctccgtaca
      721 gagccacaag cctggccagg ttgctgggat gcccgacgtt catggcgctg gagatacaat
      781 tccgggaagg caccaccttc atataattcc ctgttccgag atagttggga aattcatcat
      841 tctcattcac ggcaataata aatctccaca caggcaaacc catcttcatt gccagcaccc
      901 cgcccatcat gtcgccaaag tttccggatg gaactgaaaa aatgacatcg tcgtcagggt
      961 ttccaaccct gagcctggca aatgcataaa aataatagac gatttgcggg atcaggcggc
     1021 cgatattgat ggagttggcc gacgacaaat tcagatgcag aaggtcggga tcggcaaaag
     1081 cttccttcac cagcgcctgg caatcgtcaa acttcccttc caccgagatc acttcaacgt
     1141 ttttacccag tgtcgtcatt tgttttctct gccggagggt cacctccttc tccgggaaca
     1201 agaccaggac tttaatatta tccaggccat aaaatgcatt ggcgatcgca ctgccggtat
     1261 cgcctgaagt ggctgtaagg atcagcagtt gtttgttttc tcgggccggg taataattca
     1321 tcaggcggcc catcatcctg gcggcaaagt ctttgaagga agcagtcggc ccctggtcga
     1381 ggcgcagaat gtactttctc tcgtaaaccg gctcgagagg aaccgggtaa tcataggctt
     1441 cctccactat ctttttcaaa tcttctgccg ggatctctcc ttccaggaat ttccaggtta
     1501 catcgaatgc tatttcagga tacgatttat tgatgaaagt ataaatctct tccggggaga
     1561 tctgcgggat ggtttcgggc atgtataggc ctttatccgg tgcctggccc tgcagtaatg
     1621 cttcacggaa gctgatcgac ggtgattttc cgttggttga atagtacttt agcataaggg
     1681 ttcgtttggt tcctcaagat gattcaaagg tagaaaaaat tagtcgaggg tcaccaaccg
     1741 aaaggacaaa cggcgacaca aatgccacaa accctcacat tcatttttag cgtcttttcg
     1801 cccagctctt tacacatttc tcggcacatg aaagcgtcga agtattcgtc gcggtgcacc
     1861 gaaacgtccc agggctttcc gttgattgcg ccggccgggc accggtccat gcaaatcctg
     1921 cagttaccgc agcggcttgt ttcaaccggt tccgggctgg tatcaagcgg tgtatcgatg
     1981 agtatcgaaa ccagtctgag cctgggcccg aaagctttcg agatgaacag gtcggatttg
     2041 ccgatccagc ccaaccccgc ccgcgtagct aacattttgt gcgaaacggg ataggtcagg
     2101 ttgccggcat atacggtatc cagttcttct gtggtaacgg ttggcgagat gcatatagca
     2161 ttgattcctt ctttcttcag atcatcggct attttctgtg tcgatgcatc caattcggta
     2221 ttgatggccc tgtaatgcct gtaatactcc agtgtcgggc cgttcatgat cccgttcacg
     2281 atgccgggat ccagccttct gccaatggca atcccatagt gaaaatcgcc aaatgtctta
     2341 tcgatcagat tcttaagatc agcaaaacca tatgtgtacc tgtcatttgg agaaagatgc
     2401 ttttcgagaa tttcacgtat taattcagtt tgcatggtgt aacattggat ttatgcagtg
     2461 agggtgtctc ttcaagtgat ttttcatgct gtgggagtct ttaagttgaa ataaattctt
     2521 atctaatgga tttacttgtt agagcacata ttcatttacc tgtatattaa taactatatt
     2581 tgtggcacat agtataaacc ttgtaaaatt aaaagaattg cttggatctg cgctgcatat
     2641 cactcttgct ttagtctttt actccctggg ggtctgggct gaaaaaattg cccgttatct
     2701 caagccctgg cacgttgtgg ctttc
//
LOCUS       DAOPNH010000351         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_46147_length_2720_cov_4.754597, whole genome shotgun sequence.
ACCESSION   DAOPNH010000351 DAOPNH010000000
VERSION     DAOPNH010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46147_length_2720_cov_4.754597"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="groEL"
                     /locus_tag="PKZ74_13820"
     CDS             <1..998
                     /gene="groEL"
                     /locus_tag="PKZ74_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPM93877.1"
                     /translation="VELDDPFILIHDKKISIMKDLLPILEKAAQTGRPLVIIAEDVES
                     EALATLVVNKIRGALKVVAVKAPGFGDRRKEMLEDIAILTGGTVISEEKGYKLEDANL
                     GYLGNAEKVSVDKENTTIVGGKGQKENIDARVKQIKMQIENTTSDYDREKLQERLAKL
                     AGGVAVIHVGAASEVEMKERKDRFDDALHATRAAIEEGIIPGGGVAYLRATETINDLK
                     GENEDQTTGVAIIRRALEEPLRQIVANAGLEGSVIVQKVKEGKGDFGFNARTEKYENL
                     FSSGVIDPTKVARVALENAASVAGMLLTTECLLADAKEEKEPKMPMGGGMPDGMY"
     gene            1129..1677
                     /locus_tag="PKZ74_13825"
     CDS             1129..1677
                     /locus_tag="PKZ74_13825"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HPM93878.1"
                     /translation="MKLILSVLSVLLAINNAVSQTETNAGYTIDETTGLITYKEVVEE
                     QGTKDDLYNRCSSWLPTFYANPVEAAKVRDQSTGLIKIQHQFRLSETDKDGNIIEGNM
                     IMYNARIEFKDGRYRYVIDNFVVKEASRVPLEKWLDKKAPGYNPKWETGLGQVDAYIR
                     NELIKSLKEKMKPPKEVKEDIW"
     gene            1687..2691
                     /locus_tag="PKZ74_13830"
     CDS             1687..2691
                     /locus_tag="PKZ74_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93879.1"
                     /translation="MTDRKKDHIDLAFKSKTGTDEIDRRFYYEPLLSHHPVNDLRPFR
                     FLGHTLRAPLWVSSMTGGTELAGPINRNLARVCREFGLGMGLGSCRPLLKSDEHFADF
                     DMREIIGKDLPFYANLGIAQLESFVLNGDITPVDRLVDKLKADGLIIHVNPMQEWLQP
                     EGDLFSQPPIDTIRSFLDMAAYPVIVKEVGQGMGPESLRALLQLPLEAIELAAFGGTN
                     FAKVELLRSDETRRQYYEPLSHIGHDAFEMTEMINTIVEEKISINCRQIIISGGIQTF
                     LDGYCLISRCKLPAIYGQASGFLKYASKSYDDLYTFVKYQVDGIKLANAYFVTKDLKK
                     "
BASE COUNT          748 a          687 c          712 g          573 t
ORIGIN      
        1 aggtggaact ggatgacccg ttcatcctca tccacgacaa gaagatctcc atcatgaaag
       61 atctgcttcc gatcctggag aaagcagccc agactggtcg tccgctggtg atcatcgccg
      121 aggacgtaga aagcgaagcc ctggcaaccc tcgtagtcaa taagatcagg ggcgccctga
      181 aagttgtggc ggtaaaggcc ccgggattcg gcgacagaag gaaggaaatg ctcgaggata
      241 tcgccatcct gaccggcggt accgtaatct ccgaagaaaa aggatacaaa ctggaagacg
      301 ccaacctcgg ttacctgggt aacgctgaaa aagtgtcggt cgataaggaa aatacgacga
      361 tcgtaggagg caaaggacag aaggagaaca tcgacgcccg cgtcaaacag atcaagatgc
      421 agattgaaaa caccacttcc gattatgacc gcgagaagct gcaggaacgc ctggccaaac
      481 tggccggtgg tgtggctgtg atccatgtcg gcgctgccag cgaagtggag atgaaggaaa
      541 gaaaagaccg gtttgacgat gcactgcacg caacccgcgc tgccatcgaa gaggggatca
      601 tcccgggtgg cggcgtcgct tacctgcgcg ctaccgaaac catcaacgac ctgaaaggcg
      661 agaacgaaga ccagacgacc ggtgtggcta tcatccgccg cgccctcgag gagccgctcc
      721 gccagatcgt ggctaatgcc ggactggaag gctcggtaat cgttcagaaa gtgaaggaag
      781 gaaaaggcga tttcggattc aacgcacgca ctgaaaagta tgagaacctc ttcagctccg
      841 gtgttatcga tcctacgaaa gtggctcgcg ttgccctcga gaatgcggca tctgtagccg
      901 gaatgctgct caccaccgaa tgcctgctgg ccgatgcaaa agaggagaag gagccgaaaa
      961 tgcctatggg cggtggaatg cccgacggga tgtattagaa tttcgaatga aatctttata
     1021 aggctgccgg cattggcagc cttttttttt gcctgcccct tcaaccaaaa caatccattg
     1081 ttcgtttatt attttgtatt tttgtttaat tcatataaaa accagtaaat gaaactaatt
     1141 ctcagcgttt tgtctgtgtt gctggccatc aacaatgccg tatcacaaac tgaaaccaat
     1201 gccggatata ccattgatga aacaaccggc ctgataactt acaaagaagt cgtggaagag
     1261 caggggacaa aggatgacct ttataaccgg tgctcttcgt ggctcccaac tttttatgct
     1321 aatcccgtgg aagcggctaa agtcagggac cagtcgaccg gcctgataaa aattcagcat
     1381 cagttcagac tttccgaaac cgataaagac ggaaatatca tcgaaggcaa catgatcatg
     1441 tacaatgccc ggatcgaatt caaagacggc cgttaccgtt atgtgatcga taactttgtt
     1501 gtgaaagagg cctcccgggt tccgcttgag aaatggctcg ataaaaaggc gcccggttac
     1561 aatccgaaat gggaaaccgg cctcggacag gtcgacgctt acatccgcaa cgaactgatc
     1621 aaatcgttaa aggagaagat gaaacctccc aaagaggtga aagaagacat ttggtaaccc
     1681 gatatcatga ccgaccgaaa gaaagaccat attgaccttg cattcaaatc aaagacagga
     1741 accgatgaaa tagaccgccg gttttactat gaaccgctgt tgtcgcatca ccctgtcaat
     1801 gacctgcgtc ctttccggtt cctgggacat accctgcgtg cccccctctg ggtttcgagc
     1861 atgaccggag gtacggaact ggccggcccg atcaacagga acctggccag ggtgtgcagg
     1921 gagttcggcc tgggcatggg ccttggatcc tgcaggccgc tgcttaaaag cgacgagcat
     1981 tttgccgatt tcgacatgcg ggaaataatt ggcaaagacc tgccatttta tgccaacctg
     2041 ggtatagccc agctcgaatc cttcgtgctg aacggcgaca ttacaccggt cgaccggctg
     2101 gtggataaac tgaaagccga cgggcttatc atccatgtga acccgatgca ggagtggctg
     2161 cagccggaag gagacctctt cagccagccc ccgatcgata ctatccggtc atttcttgat
     2221 atggcagcgt atccggtcat tgtcaaagaa gtcgggcaag gcatggggcc cgaaagcctg
     2281 cgtgcactgc tgcaactgcc gctcgaagcc atcgaactgg ccgctttcgg gggaaccaac
     2341 ttcgccaaag ttgaactgct caggtcggat gagacccgcc ggcaatatta cgaaccactc
     2401 agccatatcg gccatgatgc ttttgaaatg accgaaatga tcaatactat tgtggaggaa
     2461 aagatcagca tcaactgccg gcaaatcatt atttccggcg gtatacagac gttcctggat
     2521 gggtattgcc tgatcagccg ctgcaaactg ccggccatct acggccaggc atccggtttt
     2581 ctgaagtacg cttcaaaatc gtacgacgat ctttacactt ttgtaaaata ccaggttgat
     2641 gggatcaaac tggccaatgc ctattttgtc accaaggatc tgaaaaaatg aacattaacg
     2701 gcattatcaa cggtttctcc
//
LOCUS       DAOPNH010000352         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_46286_length_2714_cov_5.599850, whole genome shotgun sequence.
ACCESSION   DAOPNH010000352 DAOPNH010000000
VERSION     DAOPNH010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46286_length_2714_cov_5.599850"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKZ74_13835"
     CDS             <1..287
                     /locus_tag="PKZ74_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93880.1"
                     /translation="ETGRPGDKETGRGLEVWPNPASQVLSVKCLGLNSGSDINLSVYD
                     VFGREVAEIKVPERQDQVQINVESYPPGVYIAVLKSGCDFLGSSKFVVAR"
     gene            complement(327..1835)
                     /locus_tag="PKZ74_13840"
     CDS             complement(327..1835)
                     /locus_tag="PKZ74_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93881.1"
                     /translation="MGLNIFTFSRNLSIVTYLLSVAFLLISFQAFARYDYNSNCRQAM
                     QSILDLKFRDARVFIANEKTVHPDNGYAIFLEHYADAIELIVTEEEELYERLIDNFED
                     RMDRMNKLDDGSPDNRWLQAEILFHTGLAQIKYGSRFGGATKLMSSYRKTREHRQKYP
                     DFWQNRKLTGIFNIILDYIPPFMRWAADMFGYSGNAELGLFQLRQYCDDAAATPGFAE
                     EGVMMAALGYKLTWGEPEGFAFLAGQPESMQSKTLVKYLYASAATYTYRNDLALSLLS
                     DIRREDLQVNFYHLDYITGRCKLNHLEPDAAVYLQKYLDEFPGLDYKKDVCNRLSFHF
                     LVHGEMERYREYREMTGRVGQELRDRDQEAVLESRSGYDPHPGLLRARLLCDGGYFEE
                     AMQVLLAIRPDEMNTPPFRLEFLYRKGRILQLSGHPAEAILELTACFEQGEKFPYTFA
                     CRSALYLGKIYEESKDSAKAAEWYEKCIGVYSTSHTTEGIREMASRALKRLK"
     gene            complement(1884..>2714)
                     /locus_tag="PKZ74_13845"
     CDS             complement(1884..>2714)
                     /locus_tag="PKZ74_13845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM93882.1"
                     /translation="IEDISKSQSPETFGIIAIVVLILIPYGSKTMIRNKQWRTEYTLY
                     KADMPYLYDSFKGNDLYANEIMKSVNRELAKPVNVLKFVDPQVKLAISHWQRAIEILP
                     EAASPYRNLGIIYSRVYKEYDTAVVYFNKTLEREPDDPLTYFNLGLAYEGMKDYGKAL
                     ENLKKSIQIDSAAINARSRLANIYYGLGEFKEAIRLNQEIMSIDPDESLPYVNIGNYY
                     IFQKDTLNGIKYYERAVELGAPADASVFLAKYYRLKGNTEKMNYYSRKADEISRKNAQ
                     "
BASE COUNT          677 a          663 c          673 g          701 t
ORIGIN      
        1 aggagacggg gagaccggga gacaaggaga cggggagagg gttggaggtg tggccgaacc
       61 ctgcttcgca agtcttaagt gttaagtgtt tagggttaaa ttcgggcagc gatattaatt
      121 tatctgtata cgatgtgttc ggacgggaag tcgctgaaat caaagttcct gagaggcagg
      181 atcaggtaca gatcaatgtg gaaagttatc cgccgggagt gtatatcgcg gttctgaaaa
      241 gtggatgcga ttttttggga agcagtaagt ttgtggtggc gaggtgatgc aggcggagca
      301 cgaattttaa aaagcaattt caaaagtcat ttcaatctct tcagcgccct ggaagccatc
      361 tccctgatcc cttcggtggt atgtgaagtt gaataaactc cgatacattt ctcataccat
      421 tctgcagcct ttgcagaatc cttgctctct tcatagattt tccccaggta cagcgccgaa
      481 cggcaggcaa aggtatacgg gaacttttct ccctgttcaa agcaggctgt taattcaagg
      541 atcgcttcag caggatggcc tgacaactga agtatgcgtc ctttcctgta aagaaactcc
      601 aaccggaaag gaggtgtgtt catttcatcc ggccggatgg ccagaagaac ttgcatggct
      661 tcttcaaaat aaccgccgtc gcatagcagc cgggccctca gcaaaccagg gtgcggatcg
      721 taaccggatc ggctctccag gacagcctcc tggtcgcggt cacgcaactc ctgccccacc
      781 ctgcctgtca tttccctgta ttccctgtac ctctccatct caccgtgaac caggaaatga
      841 aacgataacc ggttacaaac atctttcttg taatcaagcc ccgggaattc atccaggtat
      901 ttctgcaggt aaaccgctgc gtcgggctcc aggtgattga gtttgcaccg gccggtgatg
      961 tagtcaaggt gataaaaatt aacctgcagg tcctcccggc gaatatcgga taaaagactc
     1021 agcgccaggt cattccggta ggtgtaggtc gcggcgctgg cataaaggta tttcaccagc
     1081 gttttcgatt gcatggattc cggctggccc gccaggaaag caaagccttc cggctccccc
     1141 caggtcagct tgtaacccag cgcggccatc attacccctt cctcggcaaa accgggagtg
     1201 gccgcggcat cgtcgcaata ctgcctgagc tggaaaagcc cgagctctgc attgcccgaa
     1261 taaccgaaca tgtcggcagc ccaacgcata aatggcggaa tataatcgag aatgatattg
     1321 aaaatacccg tcagcttccg gttctgccaa aaatcgggat atttctggcg gtgctccctg
     1381 gtcttccggt aggaactcat cagcttggta gcgccgccga aacgggagcc gtatttgatc
     1441 tgggccagcc cggtgtgaaa aaggatctcg gcctgcagcc acctgttatc cggggaaccg
     1501 tcgtccagtt tattcatccg gtccatccgg tcttcaaaat tatctatcag ccgctcatac
     1561 agttcttctt cttccgtgac gatcagttcg atcgcatcgg cataatgttc aaggaaaata
     1621 gcatagccat tatcaggatg aaccgtcttt tcattggcga taaaaaccct tgcatccctg
     1681 aacttaagat ccaggataga ctgcatagcc tgccggcaat tggaattata atcatagcgg
     1741 gcaaaagcct gaaaagatat aagcaggaat gcgaccgaaa gaaggtatgt aacaattgac
     1801 aggttccggg aaaaagtgaa aatattcaga cccatagtca ttgtatatag ttcatttccc
     1861 gaaacttaaa atcaaagcca gaactattga gcgttcttcc tgcttatttc atccgctttc
     1921 cggctgtaat agttcatctt ttcggtatta cctttcagcc ggtaatattt ggccaggaaa
     1981 accgatgcgt cggccggggc acccagttca acggccctct cgtagtattt tatcccgttc
     2041 agggtatctt tctgaaaaat gtaataattg ccgatattca cgtaagggag cgattcatcc
     2101 gggtcgatac tcatgatctc ctggttcagg cggatggctt ctttgaactc tcccagcccg
     2161 tagtaaatat tggccaggcg ggagcgggca ttaatagccg ccgaatcgat ctgaatgctt
     2221 ttctttaggt tctccagcgc tttaccgtaa tctttcatcc cttcataggc caatccgagg
     2281 ttgaaataag tcaacggatc atccggctcc cgttcgagag ttttgttgaa atagacgaca
     2341 gccgtatcat attctttata gacccgggag tagatgatcc ccaggttccg gtaaggcgat
     2401 gctgcttccg gcaggatctc gatggccctt tgccagtggc tgatggccag ttttacctgc
     2461 ggatcgacaa atttgagcac attcaccggt ttggcgagtt cacggtttac gcttttcatt
     2521 atctcattgg catagagatc gttacctttg aaggaatcgt acaggtaagg catatcggcc
     2581 ttatagagcg tatactcggt acgccattgc ttattgcgga tcatggtctt ggatccgtag
     2641 ggaatgagga tcagcactac aatagcaata attccaaatg tttcagggct ttgtgatttt
     2701 gaaatatcct caat
//
LOCUS       DAOPNH010000353         2627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_48508_length_2627_cov_5.947900, whole genome shotgun sequence.
ACCESSION   DAOPNH010000353 DAOPNH010000000
VERSION     DAOPNH010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48508_length_2627_cov_5.947900"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="hypD"
                     /locus_tag="PKZ74_13850"
     CDS             complement(<1..626)
                     /gene="hypD"
                     /locus_tag="PKZ74_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPM93883.1"
                     /translation="MRFVDEYRDRTLVLKLAENIRSISRRKIALMEVCGGHTMSIQRF
                     GIPALLPPTISLLSGPGCPVCVSSRRYIDLAIALSRIPGVIITTYGDLIRVPGSSTSL
                     DKEKSKGADIRIVYSPMDALAVAQKNPTAEIVFLGIGFETTAPASAAAIIGAERYGLS
                     NFYLLSTHKIMPPAMAALIDEGVKIDGYIAPGHVSAITGTSIYTDVAE"
     gene            complement(629..1120)
                     /locus_tag="PKZ74_13855"
     CDS             complement(629..1120)
                     /locus_tag="PKZ74_13855"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM93884.1"
                     /translation="MNRTLYLLATAILAILSLTACRTEESKRQETIRELMDIDRRFSD
                     LSLEKGSHEAFLHYIDDSCVLLRPNRNPIIGRANIEALYKVPDTTFILTWDPLSGDVS
                     RSGDMGYTYGIFTVKMDSPAGEKVEKKGTYVTIWKKGRDGNWKFVLDTGNQGIGSPNS
                     VSD"
     gene            complement(1170..>2627)
                     /locus_tag="PKZ74_13860"
     CDS             complement(1170..>2627)
                     /locus_tag="PKZ74_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPM93885.1"
                     /translation="PIVILKNKKTGKSLAPAVSNSFETTGVMLPYMPVHYLVFERVKL
                     PAIVLTSGNLSDEPVIISNEEALSRLNKVADSFLVYNRDIYNRTDDSVLFLDVDKTRM
                     IRRSRGFAPSPVHLLKDVDGIFAAGAELVNCFCVGKGKKAFLSQHIGDLKNLETLEFY
                     RESHSRFLDMFRIKASLVACDLHPDYLSTRFAHDFAKEKGNIPLVGIQHHHAHIAACM
                     AEHGIDEKVIGIALDGVGYGTDGNIWGFEVFICDEVDFERKIHLEYIPQPGGDKANHE
                     PWRMAVSYLYHSIGADFDEEILPFSRKIGREKIDVIRTAIRNNINCPLTSSAGRLFDA
                     VSALTGLCSESMFHAEAPMRLEDCLDPDEIRRYDYSFANNIKISDMFRAILDDIRKGT
                     PASRISARFHRTVVDMLLRSSLTVRSESGINKVVLSGGTFQNRFLLKETARTLNSHGF
                     TCCFPEMIPANDGGIALGQLVIASARRKKMFIPDL"
BASE COUNT          685 a          634 c          612 g          696 t
ORIGIN      
        1 ctttcagcca catcggtgta gatcgaggtg cctgttatcg cgctaacgtg gcccggggcg
       61 atataaccgt cgattttgac cccttcatcg atcaatgctg ccattgccgg gggcataatc
      121 ttatgggtgc taagcaggta aaaattactg agaccgtatc tttcggcgcc aatgatcgcc
      181 gctgcgctgg caggggccgt ggtttcaaaa cctatgccca ggaatacaat ttccgcagtc
      241 ggattttttt gtgcaacagc cagggcatcc attggagaat aaacgatccg gatgtccgca
      301 ccttttgatt tttctttatc aagagaagta gaagacccgg gtacacggat gagatctccg
      361 tatgtagtaa tgattacgcc cggaatcctt gataatgcta tggccaggtc gatataacgc
      421 ctgctggaaa cgcaaaccgg gcagcccggg cctgacagca gggagatggt cggtggtaac
      481 aatgcaggaa tcccaaaacg ctggatggac atggtatgcc cgccacagac ctccatcaaa
      541 gctatcttcc gcctggaaat gcttcggatg ttctccgcca gtttgagtac cagggtgcgg
      601 tccctgtatt catcaacaaa cctcattttt aatcgcttac actattaggg gagccgattc
      661 cctgattgcc ggtgtcgagc acaaatttcc agttgccatc tctgcctttt ttccagatgg
      721 tgacataggt tcctttcttt tcaacttttt caccggcagg agaatccatt tttaccgtaa
      781 aaatgccata tgtatagccc atgtcacccg acctggaaac atcgccggag agaggatccc
      841 atgtaaggat aaaagtagtg tcaggtactt tatataaagc ttcaatattg gcccggccga
      901 taatcggatt ccggttaggt ctgagtaata cgcagctgtc gtcgatgtaa tgtaaaaaag
      961 cttcatggga acctttttcc agagaaagat cggagaagcg gcggtcgata tccatgagtt
     1021 cacggatggt ttcctgccgt ttactctctt ccgtccggca ggcagttaag cttagaatgg
     1081 cgaggatggc ggtggcgagc agatacagag ttcggttcat acgatttggg atagcttttc
     1141 agtcatataa aaatatataa aatgatcgat tataaatccg gaataaacat ttttttccta
     1201 cgggctgatg caataaccaa ttgcccaagg gcaatacccc cgtcattggc aggtatcatt
     1261 tccgggaaac agcatgtaaa accatgactg tttaatgttc tggcagtttc tttcagcaga
     1321 aacctgttct gaaaagtgcc accggatagc actactttgt tgatgcctga ttcagacctg
     1381 acggttaaag atgacctgag caacatgtcc acgaccgttc ggtgaaaccg tgcagaaatg
     1441 cggctggcag gagtgccctt gcggatatca tccagaatgg cacggaacat atctgaaatt
     1501 ttaatattat tagcgaagga atagtcatac cttcttattt catccggatc caggcaatcc
     1561 tcaagacgca tcggcgcttc ggcatggaac atcgattcag aacagagccc ggtcaatgcg
     1621 gatacggcat caaataaccg gcctgcactg gaggtgagag ggcaattgat gttattccgg
     1681 attgccgttc ttatcacgtc aattttttcc cttccaattt ttcgtgagaa tggaagaatt
     1741 tcttcgtcaa aatctgcacc gatggaatga taaaggtagg agacagccat cctccagggt
     1801 tcatggttgg ctttatcgcc cccgggctgc gggatgtact ccaggtggat ttttctctca
     1861 aaatcaactt catcgcatat aaatacttca aatccccaga tatttccatc cgtaccatat
     1921 ccaacgccat ccagcgcaat tccgattact ttttcgtcga tcccgtgttc agccatacat
     1981 gccgcaatat gggcatggtg gtgctgaata ccgaccagcg ggatatttcc cttttctttg
     2041 gcaaagtcat gtgcaaaacg ggtcgagaga tagtcggggt gaagatcaca ggcaaccagg
     2101 ctggctttaa ttcggaacat atccaaaaac ctggaatgcg actcccggta gaattcaagg
     2161 gtttccaggt ttttcaggtc accaatatgc tggcttagaa aagctttttt ccctttgccg
     2221 acacaaaagc agttaaccag ttcggcccct gcagcaaata tcccgtcaac atctttcaac
     2281 agatgcaccg gtgacggcgc aaatcccctc gatcgcctga tcatccgggt tttatcaaca
     2341 tccaaaaaca gcaccgaatc atccgtccgg ttatatatat cacggttgta taccaggaat
     2401 gaatctgcta ctttatttaa tcggctaaga gcctcttcat tgctgatgat cacaggctca
     2461 tccgataaat tgccgctggt cagcacaata gcaggcaatt ttacccgctc gaataccagg
     2521 taatgcaccg gcatataggg aagcataacc ccggttgttt caaagctatt gctaacggcc
     2581 ggcgccagtg attttccagt tttcttgttt ttcaggataa cgatggg
//
LOCUS       DAOPNH010000354         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_49062_length_2607_cov_5.632837, whole genome shotgun sequence.
ACCESSION   DAOPNH010000354 DAOPNH010000000
VERSION     DAOPNH010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49062_length_2607_cov_5.632837"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKZ74_13865"
     CDS             <1..754
                     /locus_tag="PKZ74_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPM93886.1"
                     /translation="GALVNMNGELVGINAAIASNTGSYAGYSFAIPANIVKKVVDDLL
                     NYGLVQRAYLGVSIREVNSKLVKEKQLATDNGVFIESVTETGGAKEAGIRQGDIIVSV
                     NAHPVRTNAELLEVIGQHNPGDIVSVLVNRDGTDKEFRVELRNQEGSTSVEKKGEDFY
                     LSDLGATLEQVPDDEKAKLSITGGLKVVELQDGMLKKGGVQKGFIILKINGIKISSRQ
                     DANYALANLRSGVIRIEGVYPNGMKMNYGFIL"
     gene            874..1740
                     /locus_tag="PKZ74_13870"
     CDS             874..1740
                     /locus_tag="PKZ74_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPM93887.1"
                     /translation="MRQLKISKSITNRETASLDKYLQDIGREELITAEEEVELARRIR
                     SGDDKALDKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRLVRLPLNQVGSLNKIKKATSRLEQEYERLPSVEEIAK
                     KLDIPEHKLDKAMRITTRYVSMDAPIAEDEDTKFLDVFVSDDTPRTDTNLMRESLNKE
                     IQRSLSTLTDKERDVINLYYGIGMNHGLTLDEIGAKFNLTRERVRQIKEKAIRRLKHT
                     SRSKLLKAYLGE"
     gene            1865..>2607
                     /locus_tag="PKZ74_13875"
     CDS             1865..>2607
                     /locus_tag="PKZ74_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPM93888.1"
                     /translation="MSESSQSKKKEQFHEYVNSLENWITDEKSGFEFVNLVGQLYYDK
                     SIELIIFRSQLIDRSSSVILKKHQHSLSVLGKELKIQVSVMLAKELLSMDIPPARIDI
                     GRLNKEWTEEGKNYLSPRAFLQDKLKNLLGKKAGYDKPVDVIVYGFGRIGRLVTRELI
                     IQGNGTQLNVKAVVTRGNSPEEITKRASLFKNDSIHGPFRGVAIEDHENKLLLINGHK
                     VKMLSANNPEDKYVPVTTFFKSSSTASAA"
BASE COUNT          799 a          576 c          609 g          623 t
ORIGIN      
        1 cggagccctg gtcaatatga acggcgaact ggtcgggatc aatgcggcca ttgccagcaa
       61 taccggttcc tacgcaggct attccttcgc cattccggcc aatattgtca aaaaagtcgt
      121 ggacgacctg ctgaactatg ggctcgtgca acgggcttac ctgggagtgt caatccgtga
      181 agtgaattcc aagctggtca aagaaaaaca actggccacc gataacggcg ttttcatcga
      241 atccgttacc gaaaccggcg gcgcaaaaga agccgggatc cggcagggcg atattatcgt
      301 ttcggtcaat gcccatcccg tcagaacaaa tgccgaactg ctcgaagtta tcggccagca
      361 caacccgggc gatattgtca gcgtcctggt caacagggat ggaacagaca aggaattccg
      421 cgtggaactg agaaaccagg aaggcagtac atcggtggaa aagaaagggg aggacttcta
      481 cctttccgat ttgggggcaa cccttgaaca ggttcccgat gacgaaaaag ccaaattgag
      541 tattaccgga ggcttgaaag ttgtggaatt gcaggatggt atgctcaaaa aaggcggagt
      601 acagaaaggt tttatcatct tgaagattaa cggaattaaa atcagttcgc gccaggacgc
      661 caattatgca ctggcaaacc tgcgaagtgg cgtgatcaga atagaaggcg tctatcccaa
      721 tggaatgaag atgaactatg ggtttatcct ttaatctatt taaaaaaatt ctaaatagcc
      781 caaaacaatt tgtatctttg cacgtttttc ttgacaaaga cggattttat ccgtatattt
      841 aaggattaca atttttgatt ttagaagaga ttcatgagac aacttaagat atcaaaatca
      901 attaccaacc gcgaaaccgc ttcacttgat aagtatctgc aggacatcgg acgggaggaa
      961 ttgattacag ccgaagaaga agttgaactg gcccggcgga tcaggtcagg tgacgacaaa
     1021 gccctggata agctggtaaa agctaatctc aggtttgttg tttccgtagc caaacaatat
     1081 cagaaccagg ggcttagcct accggacctg atcaatgaag gtaatctcgg gttgattaaa
     1141 gccgcacaga gattcgatga gactcgcgga tttaaattta tttcctatgc cgtctggtgg
     1201 atccggcaat cgatcctcca ggcactggct gaacaatcca ggctggtcag gttacccctc
     1261 aatcaggtcg gatcgctgaa taagattaaa aaggctacct cgcgcctgga acaggaatat
     1321 gaacggctcc catctgttga agaaattgcc aaaaaactcg atattcccga gcataaactg
     1381 gataaagcca tgcggatcac cacacgctat gtgtcgatgg acgcaccgat cgcagaagat
     1441 gaagacacca aattcctgga tgtatttgtt tcggacgata cgccacgaac cgatacaaac
     1501 ctgatgcggg aatcgctcaa caaagaaatc cagcgctcgc tttcaaccct gaccgacaaa
     1561 gaaagagacg tgatcaacct ttattacggt attggtatga accacggtct gaccctcgac
     1621 gagatcggcg caaagttcaa cctcacccgt gaaagggtca ggcaaatcaa agaaaaggcg
     1681 atcagaagac tgaaacatac ttcgcgcagt aaactgctga aagcctacct cggagaatag
     1741 cggttttata aaggactttt tcaggccata gatcatttac gaactatggc cttttgcttt
     1801 tttatttttg ctattattgc aacatgatct ttaaccaatt acaaacggat aaaaccacat
     1861 aactatgtca gaatcaagcc aatcaaagaa gaaggaacag ttccatgaat atgtcaattc
     1921 attggagaac tggataacag acgaaaagtc gggttttgaa ttcgttaacc ttgtcggtca
     1981 gctttattat gataaatcca ttgaactgat cattttcaga agccagttga ttgaccgctc
     2041 ctccagtgtg attctgaaaa aacatcaaca ctcgctttcc gttcttggaa aagaacttaa
     2101 gatccaggtt tcggtcatgt tggccaaaga gctgttaagc atggatatac cgcctgcccg
     2161 gatcgatatc ggccggctga ataaagaatg gacggaagag gggaaaaatt atctttcacc
     2221 cagggcattt ttgcaggata aactgaaaaa tcttttaggc aaaaaagccg gttacgataa
     2281 accggtcgat gtgatcgtat atggcttcgg ccgtatcgga cgtctggtta cccgggagct
     2341 gatcatccag gggaacggga cgcagctgaa tgtcaaagct gtcgtcacaa gaggcaattc
     2401 acccgaagag atcacaaaac gtgcctcctt gtttaaaaat gattcaatcc atggcccttt
     2461 ccgtggcgta gccattgaag accatgaaaa taaactgctg ctgatcaatg gtcataaagt
     2521 gaagatgctt tcagctaata atccggaaga taaatatgtt ccggtaacaa ccttctttaa
     2581 gtcttcatct acagcttccg cagcatt
//
LOCUS       DAOPNH010000355         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_50189_length_2566_cov_4.708483, whole genome shotgun sequence.
ACCESSION   DAOPNH010000355 DAOPNH010000000
VERSION     DAOPNH010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50189_length_2566_cov_4.708483"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKZ74_13880"
     CDS             <1..880
                     /locus_tag="PKZ74_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPM93889.1"
                     /translation="TRVSSLMPLKGLLKLEHLDFSGSMIDSLNGLENHTSLEKLNIER
                     TAVDNLLPLYSCKNLRFLYCDNTRAGKADIDSFLDHNPGCLVIYRSNPLRSWWSSLPA
                     AWKDALRTHTELSDVPTKEQLHTLAGLTSVDVSNVRGIGSLDPLTTLHRLEELNVSNT
                     GLNNINPVSSLVRLRKLDVSGNPVADLSPVSTLPVLTHLNISNTAVLKLDALDGIRSL
                     ESLNCSGTQIKKLDPLSGLVNLRKLECYGTGINNLKPLAGLTMLKQLVCYNTRLSAKK
                     IAAFKEGHPGVEVVFY"
     gene            complement(938..1645)
                     /locus_tag="PKZ74_13885"
     CDS             complement(938..1645)
                     /locus_tag="PKZ74_13885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM93890.1"
                     /translation="MEIRFDNKIVLVTGSSRGIGAAVAKKFAGLGATVIIHYSQNKKK
                     AKETFDGLHGSCHMLLQADLSVPEDVKRLFDKIIRQKEKIDILVNNAGIYEEEKWWEL
                     EFDQWQEHWKRTIETNLTGMANLSFLVAKQMIGQGYGRIINISSRGAFRGEPSALPYG
                     AAKAAMNSFGQSMAWAMADKNVFVYTLAPGWVDTDMTHDILETDRGKAVKAQSPMNRG
                     FTRRAGPCRHAPGHPRK"
     gene            complement(1659..2258)
                     /gene="thrH"
                     /locus_tag="PKZ74_13890"
     CDS             complement(1659..2258)
                     /gene="thrH"
                     /locus_tag="PKZ74_13890"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HPM93891.1"
                     /translation="MNFLCSDLEGVFIPEVWINVAEKTGIPELRRTTRDEPDYGKLMR
                     FRMALLREKGLKLKDIQDVIATIRPIDGANEMLDWIKHRMPMCIVSDTFTQFADPLIA
                     QLNRPTLFCNELIIDQEGYIAGFALRQPDQKRKVVQAMQSLNYTVIAMGDSYNDTSML
                     IQAEHGILYRPPENVIRDFPQFPVATGYDELKTLIANVL"
     gene            complement(2323..>2566)
                     /locus_tag="PKZ74_13895"
     CDS             complement(2323..>2566)
                     /locus_tag="PKZ74_13895"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPM93892.1"
                     /translation="LGAMIAEGKVHVLIFFWDPMEQQPHDVDVKALLRIAVLYNILTA
                     CNRSTADFLISSALFDEEDYDTRQKDYGDYLQRSIP"
BASE COUNT          603 a          641 c          636 g          686 t
ORIGIN      
        1 aacacgcgtc agtagcctga tgccgctgaa agggcttctc aagctggaac atctcgattt
       61 ttcaggtagc atgatcgatt cactgaacgg actggaaaat cacaccagcc ttgaaaaatt
      121 aaacatagag cgaacggctg tcgataattt attacccctt tacagttgca aaaacctccg
      181 gttcctctat tgtgacaata cccgggccgg caaagccgat atcgacagct tcctggatca
      241 taatccgggt tgcctggtga tttaccggtc gaacccgctc cggagttggt ggtcaagcct
      301 tccggctgcc tggaaggatg ctctcagaac ccatacagag ctttctgacg ttcctacaaa
      361 ggagcaactg cataccctgg cggggcttac ttctgtggat gtttcgaatg tcaggggcat
      421 tggctccctg gatccgctga caacgttgca ccggctggaa gagctgaatg tgtcaaatac
      481 agggttgaat aatattaacc cggtgtcaag cctggtgaga ctgagaaaac tggatgtgtc
      541 gggaaatccg gttgcagacc tttcaccggt cagcacgctg cctgtcctta cccatctgaa
      601 catcagcaat acggcggtgt taaaactgga tgcactggac gggattcgtt cgctcgaatc
      661 gctgaactgt tccggaactc agatcaagaa gctcgatccg ctgtcgggac tggtcaatct
      721 aaggaaactg gagtgctacg gtaccggtat caataattta aagccgctgg cggggctgac
      781 aatgctgaaa caactcgttt gttacaatac caggctttct gccaagaaga tcgcagcgtt
      841 taaggaaggg catcccggag tagaagttgt tttctattaa gatcagctcc tgaggtaact
      901 cacgccgttc atatccacaa tacaacccga catgaattca tttccgggga tggccaggag
      961 catgacggca tgggccagct cttcgggtga agcctcggtt catcgggctt tgcgctttga
     1021 cggccttccc tctgtcggtt tccagtatat catgggtcat atcggtgtcg acccatcccg
     1081 gggcaagagt atagacaaat acgtttttat cagccatcgc ccaggccatc gactggccga
     1141 acgagttcat ggctgctttg gcggcaccgt atggcaatgc gctcggctcg cccctgaatg
     1201 cgccacgcga tgagatattg atgatccgcc cgtaaccctg gccgatcatt tgtttcgcta
     1261 ccaggaagga gagattggcc atccccgtca ggtttgtttc aatggtcctc ttccagtgct
     1321 cctgccactg atcgaattcc agttcccacc atttttcctc ctcatatata cctgcattat
     1381 tgaccaggat atcgatcttt tccttttgcc ggattatttt atcaaaaagc cgttttacat
     1441 cttcgggtac cgacaagtcg gcctggagca acatatggca ggagccatgg agcccatcga
     1501 aggtttcttt tgctttcttt ttattttggg aataatgtat gatgacggtg gcgcccaggc
     1561 ctgcgaattt tttagccacg gctgcgccaa tgcctctcga agatccggta accaggacaa
     1621 ttttattatc aaatcgaatt tccatctatt ctatactctt ataatacatt tgctatcaat
     1681 gtctttaact catcataacc ggtcgctacc ggaaattgcg gaaagtccct gatgacattt
     1741 tcaggcggac ggtagagaat tccatgctcg gcctgtatca gcatgctggt gtcgttatag
     1801 gaatcgccca tggcaatgac ggtatagttc aggctttgca tagcctgcac aacttttcgt
     1861 ttctggtcgg gctggcggag cgcaaagccg gcaatatacc cctcctggtc gatgatcaac
     1921 tcgttgcaaa acaaagtggg ccggttgagc tgtgcaatca acggatcagc aaactgggta
     1981 aatgtatccg aaacgatgca catcggcatc ctgtgcttta tccagtccag catttcattt
     2041 gcaccgtcaa tcgggcggat ggtcgcgata acatcctgga tgtctttcag tttaagccct
     2101 ttttccctga gcagggccat gcggaatcgc atcagttttc cataatcggg ttcatcccgg
     2161 gttgtccggc gaagttcggg gatgccggtt ttttcagcca catttatcca cacttcgggg
     2221 ataaaaacgc cttcgagatc gctgcagaga aagttcatgg tttgggatta tcagtgttca
     2281 gggttcaatt gttcgattgt tctttatagg catggattat ctctaaggga ttgagcgttg
     2341 aaggtaatcg ccatagtctt tttgccttgt atcatagtct tcttcatcga aaagggccga
     2401 tgagatcagg aaatcagccg tgcttctgtt gcaggccgtc aggatattgt agagtaccgc
     2461 aattctgagc agcgctttga catccacatc atggggctgc tgctccatcg ggtcccagaa
     2521 aaagatgagg acatgcactt tgccttcggc aatcatggcg cccaac
//
LOCUS       DAOPNH010000356         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_50269_length_2563_cov_6.524721, whole genome shotgun sequence.
ACCESSION   DAOPNH010000356 DAOPNH010000000
VERSION     DAOPNH010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50269_length_2563_cov_6.524721"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKZ74_13900"
     CDS             <1..934
                     /locus_tag="PKZ74_13900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213989.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPM93893.1"
                     /translation="KTLEDHGAMPYTIVISATASDPAAMQFYAPFAGAAIGEFFRDTG
                     RPALVVYDDLSKQAVAYREVSLLLRRPPGREAYPGDIFYLHSRLLERAARVISADVIA
                     AKMNDLPDSLRDKVKGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETSLFNA
                     GIRPAINVGISVSRVGGSAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDPSTKAV
                     IEKGKRNVEILKQSLHETMPVEKQIAIIFCGTRGLVKNVPVPSIKDFEVEFLHHMEVN
                     HPKVLQELKAGRLNDDDVKLMEQAAKDVAKKYEK"
     gene            934..1821
                     /gene="atpG"
                     /locus_tag="PKZ74_13905"
     CDS             934..1821
                     /gene="atpG"
                     /locus_tag="PKZ74_13905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPM93894.1"
                     /translation="MPSLKEVRIRIESIKSTQQITSAMKLVAASKLRRTQNAVQALAP
                     YASKLREILGNLSASIDNTEEAVYTTVRPVQKVLLLVVTSNRGLCGPFNGNIIKAAKA
                     HIEKNYGDINRNGHLDIMTIGKKGTDFFRKNKYNLVSDNHEIFDSLTFTNTTAMATAL
                     MADFAAKKYDRIEIIYNHFKNAGQQVITIDQYLPIIPVTTGKPADCKQKADYLFEPEK
                     KVLIRELLPKMLKVQFYKIILDSFAAEHGARMTAMSQATENASEILKQLKLSYNKARQ
                     SAITKELLEIVSGAEALKG"
BASE COUNT          732 a          646 c          611 g          574 t
ORIGIN      
        1 gaagacactg gaagaccacg gcgcgatgcc atacacgatc gtcatctcgg cgaccgcttc
       61 cgatccggcg gccatgcagt tctatgcgcc gtttgccggc gccgccatcg gcgaattttt
      121 ccgcgatacc ggaaggccgg ccctcgtggt gtacgacgac ctgtcgaaac aggcggtggc
      181 ctatcgcgag gtctcactgc tgctcagaag gccccccgga cgggaagctt acccgggaga
      241 tattttctac ctgcactcga gattgctgga aagagccgcg cgcgttatct cagccgatgt
      301 tatcgccgca aaaatgaacg acctgcccga ctcgctccgg gataaagtca aaggcggtgg
      361 ttcactcact gccctcccga tcatcgagac ccaggccggc gacgtctcgg cctacatccc
      421 gaccaacgtg atctccatta ccgacgggca gatcttcctg gagaccagcc tgttcaacgc
      481 cggtatacgt cccgccatca acgtcggtat ctcggtatcc cgcgttggcg gctcggccca
      541 gatcaaatcg atgaagaaag tggccggaac gctgaagctc gaccaggcgc agtaccgcga
      601 actggaagcc ttctccaagt tcggttccga cctcgatcca tcaacaaagg ctgtgatcga
      661 aaaaggaaaa agaaacgtcg agatcctgaa acaatcgctg cacgaaacaa tgccggttga
      721 aaagcagatc gccatcattt tctgcggaac acgcggcctc gtgaaaaacg tccctgtacc
      781 aagcatcaag gattttgagg tggaattcct gcaccatatg gaggtgaacc atccgaaagt
      841 tctgcaggag ctgaaagccg gccgacttaa cgatgatgat gtgaagctga tggaacaggc
      901 agcgaaagat gtagctaaga aatacgagaa ataatgccga gtttaaaaga agttcggatc
      961 cggatcgagt cgatcaaatc gacccagcag attaccagcg cgatgaagct cgttgccgcc
     1021 agtaagctgc gccggacgca gaatgcggtg caggcgctgg ctccttatgc gtcgaagcta
     1081 cgggagatcc tcggcaacct cagcgccagc atcgacaata cggaagaagc ggtttacacc
     1141 acggttaggc ccgtgcagaa ggtgttgctg ctggtcgtta cctccaaccg cggcctctgc
     1201 ggacctttca acggcaatat cattaaagcc gccaaagcgc atatcgaaaa gaattacggc
     1261 gatatcaacc ggaacggcca cctggacatt atgaccatcg ggaagaaagg gaccgacttc
     1321 ttccggaaaa ataaatacaa tctggttagc gataaccatg agatcttcga ctccctcacc
     1381 tttacgaata caacggcaat ggccaccgca ttgatggccg attttgccgc aaaaaaatac
     1441 gaccggatcg agatcattta caatcatttc aagaacgccg gacagcaggt gatcaccatc
     1501 gaccagtatt tgccgatcat cccggtgact accggaaagc cggctgattg caaacagaaa
     1561 gccgattatc tcttcgaacc ggagaagaag gtgctgatcc gggaactgct tccgaagatg
     1621 ctgaaggttc agttctacaa aattattctg gattcgttcg ccgccgagca cggcgcccgg
     1681 atgaccgcta tgtcgcaggc tacggagaac gcatcggaaa tcctcaaaca gcttaagctt
     1741 tcttacaaca aagcccgcca gagcgcgatc accaaagaac tccttgagat cgtcagcggc
     1801 gccgaagcgc ttaaaggatg agggtggctc aaaagtacaa atattcactt ttaattgtta
     1861 gtccgacgac tgaagccgtc ggttcccacc ttcgtaaaac cataggaacc aacggcttta
     1921 gtcgttggag cacgaactaa tatcaggtca ttattgtgaa atatcaggct tttgagacag
     1981 cctttaaatc attagtcttt cgttttatat tgcgataatt tatttcgatc atagtcgaaa
     2041 taaatttcat atctttataa atatacgttc ataatcgaaa tatttattta ttgatgagat
     2101 ccaggaaatt gaaggatggg aaaaagcagt ctcgggattt ctttaaactc agggaaagct
     2161 caggtgctgg caacccgggt gaagaattca ctccactaga acgcattaat gacaattatc
     2221 ccaagttcgt tttaacaatg gataagtatt ttgatgaaaa tagagcaggt attcaatggc
     2281 agaacctggt ctactatctt gcttcccctt aaaagtagta atccgtaata gtattcggac
     2341 gattttcaat ttaacaattg aacaattgaa caattgaaca gtttaacatt ctaccttaag
     2401 tcttatgacc gctgtattct tttataataa atatctttgc aaataattta aaaactccga
     2461 accatgatat caaagaaagt cgaaaaagcc attaacgagc agataaagaa agaggagtac
     2521 tcctcccggc tttatttagc catggcatcc tggtgccaga aga
//
LOCUS       DAOPNH010000357         2496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_52291_length_2496_cov_4.555510, whole genome shotgun sequence.
ACCESSION   DAOPNH010000357 DAOPNH010000000
VERSION     DAOPNH010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52291_length_2496_cov_4.555510"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKZ74_13910"
     CDS             <1..217
                     /locus_tag="PKZ74_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93895.1"
                     /translation="LFRKQFILYPVYSGWFVMIKIREQKEPLIGGFGGGLRTRLLGYF
                     IRADLAWGVEELKIKKPVFYFSLSLDF"
     gene            774..>2496
                     /locus_tag="PKZ74_13915"
     CDS             774..>2496
                     /locus_tag="PKZ74_13915"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPM93896.1"
                     /translation="MPKVLQPYPYEEVQKAATEYFKGDTLAANVWINKYALKNSANQI
                     FELTPDDMHWRLAKEIARIERKYPNPMTEDEIYFLLKDFRYIVPQGGPMAGIGNDFQI
                     SSLSNCFVIGNKGNSDSYGGIMKTDQEQVQLMKRRGGVGHDLSHIRPTGSPVKNSALT
                     STGIVPFMERYSNSTREVAQDGRRGALMLSISSKHPDAEMFIDAKLEQGKVTGANVSV
                     KIDDEFMNAVKNNTTYVQQFPIVSDNPVVRKEIDAMQLWNKIIHNAWRSAEPGVLFWD
                     TIKREAVPDCYADLGFATVSTNPCGEIPLCPYDSCRLIAINLYSYVENPFTQHAVFNG
                     SLFARHARHALRIMDDIIDLELEKIDRILDKIITDPEDEDVKLIEKNLWLNIREKCIQ
                     GRRTGIGITAEGDMLAALGLRYGSEEAISFSTEVHKMLALEACRSSVDLARDRGPFPV
                     YSTEREKNNPFINRIREADPELYEDMKKYGRRNIAMLTIAPTGSVSICTRTSSGIEPV
                     FSVSYRRRRKVNPNDLDVRIDFVDEVGDSWEEYNVFHPKFEEWLKINGYDTEEVKAYH
                     EDELNRLI"
BASE COUNT          672 a          608 c          622 g          594 t
ORIGIN      
        1 cctattccga aaacaattca ttttatatcc ggtatattca ggatggttcg ttatgatcaa
       61 gatcagggaa cagaaggagc cgctgatcgg cgggttcggg ggtggtttgc ggacccggtt
      121 gctcggttat tttatccggg ccgacctggc ctggggcgtg gaagagttaa agatcaaaaa
      181 gcccgttttc tatttttctt taagtcttga cttttaatgg aagatctcat gaaggctgtt
      241 cattattttc tgatttatga agtattacct gttgaaaact atatttcgtt ttttccttgt
      301 tctttattat cctcctgata atcttgaatt tggcatgatg ataatgacag gcgttattcc
      361 gtatagcatt taaaatattt atcaataatt gttgaaaaaa gggtttgaga taacgtataa
      421 cgtttaatat catcatatta agatttttca accccttaat gttgaaaagt tcattaggaa
      481 aatcctttct tcttatagta ttttagcctt agagaatttt ccccaggaat gcttacttaa
      541 acagttgatt tgtaatcgtt aaggcgtagg taattttatt aatcaaaggc gagatgaagg
      601 aattggaaaa cacactattc gacagcattc aaaaggcaga agagaaggac caggccatta
      661 gtaatggtgc cgattatttt cagagcatgc tggagcacag gcaacgccct gcaacggata
      721 aggttgcaaa gcaaagcctg atcgggctgg cggatgagaa taattcggaa ccaatgccaa
      781 aggtcctgca accctatccc tatgaagaag tccagaaagc agccacggaa tactttaaag
      841 gagataccct ggcagcaaat gtgtggatca ataaatatgc cttgaaaaac agcgccaacc
      901 agattttcga gctgacgccc gacgatatgc actggcgcct ggcaaaggaa attgcccgca
      961 ttgaaaggaa atatcccaac ccgatgaccg aggacgagat ctatttcctt ttgaaagatt
     1021 tccgctatat cgttccgcaa ggggggccga tggccggcat cggcaatgat tttcagattt
     1081 cttcactctc caattgtttt gtcattggca acaaggggaa ttccgattca tacgggggca
     1141 tcatgaagac cgaccaggag caggtacagc tgatgaaacg gcgcggtggc gttgggcacg
     1201 acctgtcgca tatccgcccg acgggaagcc ctgtgaagaa cagcgccctt acttcgaccg
     1261 ggatcgtgcc cttcatggag cgctattcca acagtacccg cgaagtggcc caggatggcc
     1321 gccgcggcgc actcatgctc tccatctcca gcaagcatcc cgatgccgag atgtttatcg
     1381 acgcgaagct cgaacagggg aaggtaaccg gcgccaatgt ttcggtcaag atcgacgatg
     1441 aattcatgaa cgccgttaag aacaatacaa catatgtcca gcagttcccg atcgtctccg
     1501 acaatcctgt ggtgcgaaag gagatcgatg ctatgcagct ctggaacaag atcattcaca
     1561 atgcctggcg ttcggccgag ccgggtgtgc tgttctggga tacgatcaaa cgcgaagccg
     1621 ttcccgattg ctacgccgac ctgggctttg ccacggtgag taccaacccc tgcggcgaga
     1681 taccgctctg cccgtacgac agctgccggc ttatagcaat caacctttac agttacgtcg
     1741 agaacccatt cacccagcat gcggtattca acggcagcct ctttgcccgt catgcccgac
     1801 acgccctccg gatcatggat gatatcatcg acctggaact ggaaaaaata gaccgtatcc
     1861 tggataagat cattacggat ccggaagatg aggatgtcaa actgatcgaa aagaacctct
     1921 ggctgaacat ccgcgagaag tgcatccagg ggaggcgcac cggaattggc atcaccgccg
     1981 aaggcgatat gctggccgca ctcgggctgc gctatggttc cgaagaagcc atcagcttct
     2041 ctaccgaagt gcataaaatg ctggccctgg aggcatgccg ctcctcggtc gacctggccc
     2101 gcgaccgcgg cccgttcccg gtctacagca ccgaacggga aaagaataat ccgttcatca
     2161 atcgtatccg cgaagccgat cccgagctgt atgaggatat gaaaaaatac gggcggcgaa
     2221 atattgccat gctcaccatc gccccgaccg gtagtgtgag catctgcacc cgtacctcct
     2281 ccggcatcga accggtattc tccgtttctt accgccgcag aaggaaagta aacccgaacg
     2341 atctcgacgt ccggatcgat tttgttgatg aagtcggcga ttcgtgggaa gaatacaacg
     2401 tattccatcc gaaattcgag gagtggctga aaatcaacgg gtacgacacc gaagaggtaa
     2461 aagcatatca cgaagatgag ctgaaccggc tgatcg
//
LOCUS       DAOPNH010000358         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_53434_length_2459_cov_4.632696, whole genome shotgun sequence.
ACCESSION   DAOPNH010000358 DAOPNH010000000
VERSION     DAOPNH010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53434_length_2459_cov_4.632696"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PKZ74_13920"
     CDS             <1..963
                     /locus_tag="PKZ74_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93897.1"
                     /translation="VSESSTITKQVQVGLREFHHLGGHVFASYFPIDYGLAYLYLYDD
                     HNNLIPVDTTRIDTLGYYFFYEVPVGKYLTKTRLESNSVHYGQFMPTYFRNAIDWNDA
                     TPIIVDDNDNWECDINLRPSAGLGAGSGEILGQISYDTSLVVRTLVPAGNIEIVLMDA
                     KGGCLTCTLSDMEGYFLFNDLAYGTYQLFPDVAGVTSTPMYITISEENPALDDISLVI
                     YPGEITFSINENVSDYVGNAMMVYPNPVNDQARLSVEIKKNTTATVVITDIAGRMVYR
                     QETRLSEGMQNIVIPVRDLQKGIYQVTLIPEDRVLVSGKFLKMD"
     gene            1054..1644
                     /locus_tag="PKZ74_13925"
     CDS             1054..1644
                     /locus_tag="PKZ74_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM93898.1"
                     /translation="MNSDEQIISGCREGKRKAFSKLFDTYAPVMMGVCMRYCKNRIDA
                     EDVMQDGFIKVFGQIHKFRGDGSLEGWIKRIMINAAIDSYQANLKHAFHEDVTENKLS
                     AQIADMDDHCDDDLPPELQIPHKKLMEMIQELPDGYRMVFNLFAIENYSHKEIAALLG
                     ISENTSKTQLLKARKALRRKIEAIIEHSHINRISTV"
     gene            1650..>2459
                     /locus_tag="PKZ74_13930"
     CDS             1650..>2459
                     /locus_tag="PKZ74_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93899.1"
                     /translation="MNKIDDLFREAMTGYKMTPAPGLWLRIERHFFPPSKYRPSGLIT
                     SVLLLSIAGLMPWILIPANDQDEKKPVLPEGNFKRGYLIETITPVEIKPDGNGDYRIS
                     ARSFTVRPTVYEEKEAIPEAGASEQFIASINDPADDPAIRPILIANRQHIADLAEPAI
                     PNTAVKEGLLKDASIAFLESHPVGLIQYSRYIEEMKISAELRKTSLTPYHEKSYVRKG
                     EFSAGLNFNPSIVFYDPNPYNKMLGGDATISYKMFSFSIMTGIGYSRMEDVS"
BASE COUNT          654 a          604 c          590 g          611 t
ORIGIN      
        1 gtttccgaat cgagcaccat caccaagcag gttcaggtcg ggctgcgcga attccaccac
       61 ctgggcgggc atgtgttcgc cagctacttc ccgatcgatt atggtctggc ttacctctac
      121 ctgtatgatg atcacaacaa tctgatcccg gtagatacga cacggattga taccctggga
      181 tattatttct tctatgaggt cccggtagga aaatatctca ccaagacccg gctggagtcg
      241 aattcggtgc attatggcca gttcatgcca acctatttcc gcaatgccat cgactggaac
      301 gatgccactc ctatcattgt cgatgataat gataactggg aatgtgatat taatttacgt
      361 ccgtcggcag gattaggcgc tggttcgggc gagattcttg gccagatatc ctatgacacc
      421 tcgcttgtag tcagaaccct ggtccctgca ggcaatattg agattgttct catggatgcc
      481 aaagggggct gccttacctg taccctgtcg gatatggaag ggtatttcct gttcaacgac
      541 ctggcatacg ggacatacca gttgttcccg gatgtagccg gcgttacttc caccccgatg
      601 tatatcacca tctccgaaga aaatccggca ctggatgata tcagcctggt tatttatccc
      661 ggagaaataa ccttctccat caatgaaaat gtttcggatt atgtaggaaa tgccatgatg
      721 gtttatccga acccggtgaa cgaccaggcc aggctttccg tagaaataaa gaaaaacacg
      781 acagctactg ttgtcatcac cgatattgcc ggccggatgg tttaccgcca ggaaacccgg
      841 ctctccgaag gaatgcagaa tatcgtcatc cccgtaaggg atctgcaaaa aggaatttac
      901 caggtcaccc tcatcccgga agaccgggtg ctggtttcag gcaaattcct caaaatggac
      961 tgatgttttt tcccgattat ttttggtatt tacagcacct ccgggcaact tttcttattt
     1021 attcccgtct gacttaatgt aagatcttac aggatgaatt ccgatgagca aatcatatcc
     1081 ggttgccgtg aaggcaagcg aaaagctttc agcaagcttt tcgacaccta tgcaccggtg
     1141 atgatgggtg tttgcatgcg atactgcaaa aaccggatcg atgcggaaga tgtgatgcag
     1201 gatggattta tcaaggtatt cggccagatt cacaaatttc gcggcgacgg ctcccttgag
     1261 ggatggatca aacggatcat gatcaatgcc gctatcgaca gttaccaggc caatcttaag
     1321 cacgctttcc acgaggatgt gacggagaat aagctcagcg cccagatagc cgatatggat
     1381 gaccactgtg atgacgacct gccgccggaa ttacagatcc cgcataaaaa actgatggaa
     1441 atgatccagg aactgcccga cggttacagg atggttttca acctcttcgc cattgaaaat
     1501 tacagtcata aggagattgc cgctctgctg gggatttcgg agaatacatc gaaaacccag
     1561 cttctgaaag cgagaaaagc actccgccgg aaaatagaag ccattataga acacagtcat
     1621 atcaacagga tatcaacggt atgaacaata tgaacaaaat agacgatctt ttcagggaag
     1681 ccatgaccgg ctataagatg acgcctgccc ccggtctctg gctgaggatc gaaaggcatt
     1741 ttttcccgcc ttccaagtac aggccatccg ggctgatcac ctcagtcctg ctcttatcta
     1801 ttgccgggct tatgccgtgg atactgatcc cggctaatga ccaggatgag aagaagccgg
     1861 ttttgcctga aggcaatttc aaaagaggtt atctgatcga gaccattacc ccggttgaga
     1921 tcaaacccga tggaaacggc gattaccgga tatcggccag aagttttacg gtcaggccga
     1981 ctgtttatga agagaaagag gccattcctg aggccggtgc ttccgaacag ttcattgctt
     2041 caatcaatga tcccgctgac gatcctgcca ttcggcccat cctcatagcc aaccggcagc
     2101 atattgccga tctggccgaa ccggctatac ccaatacagc cgttaaggaa gggttgctga
     2161 aagatgcaag cattgctttc ctggagtcgc atcctgtcgg actgatacag tacagccgtt
     2221 atattgaaga aatgaagatc agtgccgaac ttcgtaaaac ttcgctgact ccctaccatg
     2281 agaagagtta tgtcagaaaa ggtgaattct cggccggtct taatttcaac ccttccatcg
     2341 ttttctatga tccgaacccg tataataaaa tgctcggcgg ggatgcaacc attagctata
     2401 agatgttctc cttcagcatt atgaccggta tcggttattc gcggatggaa gatgtgagc
//
LOCUS       DAOPNH010000359         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_55493_length_2395_cov_6.519231, whole genome shotgun sequence.
ACCESSION   DAOPNH010000359 DAOPNH010000000
VERSION     DAOPNH010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55493_length_2395_cov_6.519231"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..166)
                     /locus_tag="PKZ74_13935"
     tRNA            complement(93..166)
                     /locus_tag="PKZ74_13935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Ile,seq:cat)
     gene            508..963
                     /locus_tag="PKZ74_13940"
     CDS             508..963
                     /locus_tag="PKZ74_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPM93900.1"
                     /translation="MGRGNESFGKKDVRSKKEKKRKEKEQKRLERKENKGATHPDDMI
                     AYVDEFGNITSTPPDRAQRTTVNAEDVAIGVPKAAELNPEDLLRKGIVSFFDESKGFG
                     FIKDLETKQDIFVHINGLLQPVREGNAVTFEVVKGKKGPNAVNVKLKTD"
     gene            complement(1013..2035)
                     /locus_tag="PKZ74_13945"
     CDS             complement(1013..2035)
                     /locus_tag="PKZ74_13945"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HPM93901.1"
                     /translation="MENTSNPAQSDTSWQNIILRYNKPRPSASLFQVVNSFGPYLLLW
                     IIMILLIPVSPWLALALTIPASGFLVRIFIIFHDCGHGSFTRSKKMNRIIGMIAGILV
                     FTPYDRWTDSHRTHHATVGNLDKRGIGDVWTLTVDEYLKRSPRDRFIYKVFRNPIIMI
                     GIGGFLMFMLGNRFTRKSNTRKQKLNIYLTNAIIAVLITGMIWLTGWKVYLMIQLPVM
                     YFASVAGVYLFYLQHQYEEVIWERDAEWNYKEMALHGSSFFKLPGLLRWFTGNIGYHH
                     IHHLGPTIPNYNLVKCHNENPVFQEVRPINFFRSLRSLSVRLWDEERKKIITFRQLRR
                     YYPAGR"
BASE COUNT          700 a          539 c          570 g          586 t
ORIGIN      
        1 gatatctgcc atgcaaattg tcgaatcgtc aaatgaaaaa gccgctcttt gccttattaa
       61 aaacttaaac ggacaaagga gcggcttttt tgtgggccca cctgggctcg aaccagggac
      121 ctactgatta tgagtcagtt gctctaaccg actgagctat aggcccgaac atcatcattt
      181 gacgatttga cgatttggag atttgacgat ttacgattta cgatttgggg atgttgcgat
      241 tttattaaat tgtcgaatct tcaaatttcc gaattgtcaa ttttcgggag tgcaaaagta
      301 gggaaaaatc agatgaaaat ccatactcgc gggtaagggc ctgaaaaaat attccatttc
      361 tgccactctg tcaaattctt ccggattatt tcgtgccatt tgaataaaga aggttacctt
      421 tatgcctgcg gtcaaacaaa ataaacccta taagcaataa gcatccgtta tacatattaa
      481 atatctgtaa ttcaatataa taaacctatg ggaagaggaa atgaatcgtt tgggaaaaaa
      541 gatgtccgtt caaaaaaaga gaagaaaaga aaagaaaaag aacaaaaacg cctggagcgg
      601 aaagagaata aaggggctac ccatcccgac gatatgatcg cttacgtcga cgaattcggc
      661 aatatcactt ccactccgcc cgacagggca caacggacca ccgtcaatgc cgaagatgtg
      721 gcaatcggtg tgccaaaggc tgcggaatta aaccctgaag atctgctgcg caagggcatt
      781 gtatcgttct ttgatgagag caaaggcttt gggtttatca aagatctgga gaccaaacag
      841 gatattttcg tccatatcaa cgggctcctc caaccggtcc gcgaaggcaa cgcggtgaca
      901 ttcgaagtgg tcaaagggaa aaaagggccg aatgcggtga atgtcaagct gaaaacagac
      961 tgaatccgaa agtcaccagg gttcgatcaa tgactgaaaa ccggtcggtc gcttaccggc
     1021 cagcagggta ataccggcgc aactgcctga aggtaattat ctttttacgc tcctcatccc
     1081 acaatctgac cgataaagac cgcaggctcc ggaaaaaatt aatcggcctg acctcctgga
     1141 aaaccggatt ctcgttatgg catttcacca gattgtagtt aggtatcgtc ggccccaggt
     1201 gatgaatatg gtgataaccg atattccctg taaaccagcg caataacccg gggagcttaa
     1261 aaaaagaact gccgtgcagg gccatttcct tatagttcca ctccgcatct ctctcccaga
     1321 tcacctcttc gtactggtgc tgcagataaa acaggtaaac gccggctacc gaggcaaaat
     1381 acatcacggg caactggatc atcaggtaaa ccttccatcc tgtcagccag atcatgccgg
     1441 ttatgagtac ggcaataatt gcatttgtta gataaatatt cagcttttgt tttctggtgt
     1501 tggattttcg ggtaaaccgg ttcccgagca tgaacatcag gaacccgcct atgccgatca
     1561 tgatgatggg attccggaaa accttgtaga tgaaccggtc acggggcgat cttttcagat
     1621 attcgtcaac ggtcaatgtc catacgtcgc cgattcccct tttatccagg ttccctaccg
     1681 ttgcatgatg cgtccggtgg ctgtctgtcc agcggtcgta gggggtaaaa acaaggatgc
     1741 cggcaatcat gccgatgata cggttcattt tctttgacct ggtaaaggat ccgtggccgc
     1801 aatcgtggaa gatgatgaat atacggacca ggaaccccga tgccgggatg gtcaatgcca
     1861 gggccagcca gggagaaacc ggtatcagaa ggatcatgat aatccacaaa agcaaataag
     1921 ggccaaatga attgacaacc tggaacaggc tcgcggatgg cctgggcttg ttatagcgca
     1981 gaatgatgtt ttgccaggat gtatcggatt gagcggggtt tgatgtattt tccatttcag
     2041 tcggtataga atgatcatta tttatgcaaa tcctgcataa ggacgttcaa aagcaaaccg
     2101 gctttttaaa tggaatttga atttaaagag aaggtcgttg cgaatacaaa agtatgcaac
     2161 tttttggaat attcttacga aatcagcaaa ataccagcaa tcatagcgac ttcacctgtt
     2221 aatcctgcaa caatccgggc atcgggcctg cgaaaatccc tttcggggag gcgaagctca
     2281 tcgccggaac ccctccctgg caagctccgc ggagtgttac aaatttcgat cccctgacaa
     2341 cctggaatcc ctggttgagg aaaatctggt cggaggccct gtaaatggcc tcctg
//
LOCUS       DAOPNH010000360         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_55900_length_2383_cov_4.018900, whole genome shotgun sequence.
ACCESSION   DAOPNH010000360 DAOPNH010000000
VERSION     DAOPNH010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55900_length_2383_cov_4.018900"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKZ74_13950"
     CDS             complement(<1..1093)
                     /locus_tag="PKZ74_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HPM93902.1"
                     /translation="MFKRYLLFFVLIVLIASQSAGQGLLCEESEPFCTGSIYTFPAGT
                     TGQAQYGAYYDCLSTQPAPAWYHLLIDDPGSITIYMYSTPLVDIDFCCWGPFTDPWEP
                     CVQGLTANTVVDCSYSPAPTEYCVIPNGQTGQYYILLITNYSQQPCEITFSQTAGNGS
                     TDCTILPPPVGNNGPLCVGETLQLYADTINNAQYYWSGPNGFLSTQQNPIIPNVTLNN
                     AGAYSCVITVNGQSSDPAITNVVIYSLPGAVLTSPDTTVCPGEPAYAIFNFTGWGPFK
                     VTYNNGSNTFIQPGLYGPVDTIFLYPPVPTMYTFTKVEDLHCAKMLLFMDMYVNTYPE
                     TSGTMTGSAEICAGEPVELTFELTGSPPWD"
     gene            complement(1102..1509)
                     /locus_tag="PKZ74_13955"
     CDS             complement(1102..1509)
                     /locus_tag="PKZ74_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM93903.1"
                     /translation="MSAFSFGQTEVTNTSVQDLVHYDLSFNQDDFGARFSVLSHSDDN
                     YDYYAVDLTKFNDDFERVYFMVLTFTDKRIVNLDSDILQKDQTWFKSYYTSKEEEITC
                     LFNELREKTTEAGLRMSKEEKDVWMTRNNKYKK"
     gene            complement(1648..>2383)
                     /locus_tag="PKZ74_13960"
     CDS             complement(1648..>2383)
                     /locus_tag="PKZ74_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM93904.1"
                     /translation="STTITAYGFGGNYGTYTITWFDGAEIVKVDYQSPSTLIVSPDDE
                     GPHIYNVEIDDGFNTSSNFLKITVFPSPVFNILGSPQIISCPTDTVRLSPDQLIPGAE
                     YYWSNGSTDVSLPVVSTGIGFNSRTFDLTVTNLQGCVHKDTVTVVFDFTACTGIDEFG
                     EDPGINLYPNPSEGIINVEFLDASGLNTMQIINPYGVVVLNRPITNPGNDFLKTEINL
                     AGLPSGIYFFKALFNRNLSYHKVIIR"
BASE COUNT          658 a          511 c          569 g          645 t
ORIGIN      
        1 tatcccacgg cggggatccg gtcagttcaa atgtcagttc taccggttct ccggcgcata
       61 tctccgccga tccggtcatg gtgcccgatg tctccggata ggtgtttaca tacatatcca
      121 taaacaaaag cattttcgca caatgcaggt cttctacttt tgtaaaggta tacatagttg
      181 gtacaggggg atagaggaag atagtatcca ccgggccgta taatccgggc tggataaagg
      241 tattagagcc attattatag gttactttaa aaggccccca tcctgtaaaa ttgaatatgg
      301 cataagccgg ttcacccggg cagacagttg tatcgggact tgttaagact gctcccggca
      361 ggctgtaaat gacgacgttg gttattgccg gatcgctgct ttgtccgttt acggtaatta
      421 cacaggaata agcccctgcg ttattcagtg ttacattggg gatgattgga ttttgctggg
      481 ttgacaggaa tccgttcggc ccgctccagt agtactgggc attattgatc gtatcggcat
      541 aaagctggag ggtttcgccg acacaaagcg gcccgttatt ccctacggga ggcggaagga
      601 tcgtacagtc tgtgctaccg ttgccggcgg tctggctgaa tgtgatctcg cagggctgct
      661 gtgaatagtt ggtaatgagg aggatataat actgaccggt ctggccgttg ggaataacac
      721 aatactctgt tggcgcgggt gaataactgc aatctacaac cgtattagcc gtaagtccct
      781 ggacgcaagg ctcccaggga tcggtgaacg ggccccagca gcagaaatcg atgtcgacca
      841 gcggggtgct gtacatgtaa atcgtaatac ttccgggatc atcgatcagc aaatggtacc
      901 aggcaggagc cggctgggtg gaaagacagt cgtaataagc accatattgc gcctgccctg
      961 tcgtaccggc cgggaaggta taaatcgaac ccgtgcagaa aggttccgac tcttcgcata
     1021 acaaaccctg cccggcgctt tgagaagcta taagaacgat cagaacaaaa aaaagtaaat
     1081 atcgcttaaa catgttcgtc atcatttttt atatttattg ttcctggtca tccaaacatc
     1141 tttctcctct ttcgacattc tcaatccggc ctctgttgtc ttttccctca gctcattaaa
     1201 gagacaggta atttcttctt ccttactcgt ataataactt ttaaaccagg tctgatcctt
     1261 ctgtaaaata tcggaatcca ggtttacaat ccttttatcg gtgaaggtca gtaccatgaa
     1321 atagacccgt tcaaaatcat cgttaaattt tgtcagatca actgcataat aatcataatt
     1381 atcatcggaa tggctcaaaa ccgaaaacct ggcaccgaaa tcatcctgat taaaggaaag
     1441 atcataatgc accaggtcct gaacagaagt attggtaact tccgtctgtc cgaatgaaaa
     1501 tgcggacatg attgcgaaaa gtatgatcaa acaagttctc attaagggta aaatgtttaa
     1561 gaatgaatat atcttttttt aaagcgggct aagataacta tatttttaat caagggtatg
     1621 atattgaacc ttatcgggta aaaaagttta ccttataatg accttgtgat aacttaaatt
     1681 tctgttaaat aaagccttga agaaataaat acccgatggc aggccagcca gattgatttc
     1741 ggttttcagg aaatcattac cgggatttgt gattggccgg ttcagcacta ccactccgta
     1801 tggattgata atttgcatgg tattcaaacc ggaggcatcc aggaattcaa cgttaataat
     1861 accttcggaa gggttggggt aaagattgat tccgggatcc tcgccaaatt cgtcgattcc
     1921 tgtgcaggca gtaaagtcaa acacaaccgt aacggtatct ttgtgaacac atccctggag
     1981 atttgtaacg gtcaggtcga atgtcctgga attaaatcct atgccggttg agacgaccgg
     2041 cagtgagaca tcggtacttc cgttcgacca gtaatattct gcgccgggga taagctgatc
     2101 gggcgaaagc ctgacggtgt cggtcgggca ggatatgatc tgcggagagc cgaggatatt
     2161 gaagacaggt gaagggaata ctgttatttt cagaaagttg gaggatgtat taaatccatc
     2221 gtcgatctcc acattataaa tatgtggtcc ttcgtcatcc gggctaacga tcagcgtaga
     2281 agggctttga taatcgacct taacaatctc ggcgccatca aaccatgtta tcgtataggt
     2341 tccataatta ccaccaaacc cgtatgccgt gatggtggtg ctt
//
LOCUS       DAOPNH010000361         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_56385_length_2369_cov_7.395419, whole genome shotgun sequence.
ACCESSION   DAOPNH010000361 DAOPNH010000000
VERSION     DAOPNH010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56385_length_2369_cov_7.395419"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKZ74_13965"
     CDS             complement(<1..613)
                     /locus_tag="PKZ74_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM93905.1"
                     /translation="MGKVIALANQKGGVGKTTTAINLGASLAVLEYKTLLVDADPQAN
                     TTSGVGFDPKNIKTSVYECIIDDLDPNNILLNTQTPNLDLLPAHIDLVGAEIEMINLP
                     NREKMMRRVVQKVRHKYDFILIDCSPSLGLVTINALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQTRLNPELDIEGILLTMFDQRLRLSKQVAEEVKTHF"
     gene            complement(758..1123)
                     /locus_tag="PKZ74_13970"
     CDS             complement(758..1123)
                     /locus_tag="PKZ74_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPM93906.1"
                     /translation="MEEFGNKEIRKEEVFSRVIRAGKRTYFFDVKATRSGENYLTITE
                     SKRRFDNEQGKFFYEKHKIFLYKEDFEKFLTGLNQVMHFVETGEKPEGIEDEAFSGEE
                     AHTDFDFDDLGDHSKDTDH"
     gene            1345..2316
                     /gene="nusB"
                     /locus_tag="PKZ74_13975"
     CDS             1345..2316
                     /gene="nusB"
                     /locus_tag="PKZ74_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPM93907.1"
                     /translation="MLSRRHLRVKALQALYAFFQSDGQDLVAGEKQLLRSTDKLYELY
                     IWQLSFLVRFADYSLNRIEEAKKKFYPTEEDLNPNTRLVDNKIIAQIDKNRDFRRWHE
                     KLKINWSDEENLFHKLFQELKTHEEYNKLIKLPYKGYQDDKELLNFIIQNLLPDFELL
                     RMLYEDQSIYWSDEDYDISLMMLLKTVRIFREHWGPEHSLPTLFKSEEDEEEDELDDR
                     QYMIKLFRYVILRNTETGKMIEEQAENWESERIAVMDMIIMKMALTELMIFPEIPVKV
                     TINEYIEISKSYSSIKSKLFINGILDKLVVKLKEEGKIKKSGRGLLE"
BASE COUNT          677 a          504 c          550 g          638 t
ORIGIN      
        1 ggaaatgggt tttgacctcc tcggccacct gtttggaaag gcgcagccgc tgatcgaaca
       61 tggtcagcag gattccttcg atgtcgagtt caggattcag cctcgtctga acgatcttaa
      121 tggtattgag cagttttccc aggccttcga gggcgaaata ttcgcattgt accgggatga
      181 tcactgaatc ggcggctgtt aaggcgttga tcgtaaccag gcccagcgaa ggcgagcagt
      241 cgatcaggat gaaatcatac ttgtgcctga ctttctgcac gacccgacgc atcattttct
      301 cccggttggg gaggttgatc atctcgattt cagcacctac caggtcgata tgcgcaggca
      361 acagatccag gttcggggtt tgcgtattga gaaggatatt gttgggatcc aggtcatcaa
      421 tgatgcattc ataaacgctg gtcttgatgt ttttgggatc aaaacccacg cccgatgtgg
      481 tattggcttg cggatcggca tcaaccagaa gcgttttgta ttcaagaaca gccaggctgg
      541 cgccgagatt gatcgcagtt gtggtttttc cgacgccccc tttctggttg gctaatgcaa
      601 tgaccttacc catggtttat ttttcttgaa cggtcaaaaa taaggtaaaa aacggcagtg
      661 gcttgcctgg aattatgata atttatcaac tgatgaaatg ctctaaaaat aagcatgttg
      721 acggcacgcg ggaaaaacat gcgaacgccg gatagaatca atgatctgta tctttactat
      781 ggtctcccag atcatcaaaa tcgaagtcgg tatgggcctc ctctccacta aacgcctcgt
      841 cttcaattcc ttccggtttt tcgccggtct ctacaaaatg cattacctgg ttgaggcctg
      901 tcaggaactt ctcaaaatct tctttgtaaa gaaaaatctt gtgtttctcg taaaaaaact
      961 ttccctgctc attgtcaaac cgtcttttgc tttcggtgat cgtaaggtaa ttctcacctg
     1021 aacgggtagc cttcacgtca aaaaagtagg tccgtttccc ggcacgaatc accctggaaa
     1081 aaacctcttc ttttctaatc tctttgtttc caaactcttc catcttcttt tccgttttta
     1141 ttccttcaga tttatcacaa aaataaaaat atttcttcac acaacacttt catgcttttt
     1201 tgcggctgat tggctggaga ctctcctaag acagcaaaat aacttcaaag gttgcattta
     1261 tattttgtgg caggagataa aaaattccga cgcggtgccg gaaaagttta cctttgtagc
     1321 tttaaattca gttcatttcg aatcatgctg agcagaaggc acctcagggt caaagcatta
     1381 caggctttgt acgcgttttt ccagtcggat ggccaggatc tggttgcagg agaaaaacag
     1441 ttactccgca gtaccgataa attgtacgaa ctttatatct ggcaattatc cttcctggtc
     1501 cgttttgccg attattccct caaccggata gaagaagcaa aaaagaaatt ttatcctacc
     1561 gaagaagacc tcaatccgaa tacccgcctg gttgataaca agatcatcgc ccagatagat
     1621 aaaaacaggg atttcaggcg ttggcacgaa aagctgaaga tcaactggtc ggatgaagaa
     1681 aacctgttcc acaaactctt tcaggagctg aaaacccacg aggaatacaa taaactgatc
     1741 aaattacctt ataaaggata ccaggacgat aaagagctcc tgaacttcat tatacagaac
     1801 cttttacccg attttgagct tctcaggatg ctatatgaag accagagcat atactggtcg
     1861 gatgaagact atgatatttc gctgatgatg ttgttgaaaa cggtcagaat cttccgcgag
     1921 cattggggcc cggaacactc tttgccaaca ttgtttaaaa gcgaagaaga tgaggaggaa
     1981 gatgagctcg atgaccggca atacatgatc aagcttttca ggtatgttat actgcgcaat
     2041 accgaaacgg gcaaaatgat cgaggaacag gcagagaact gggaatcgga gcggattgcg
     2101 gtgatggata tgatcattat gaaaatggca ctcaccgaac tgatgatttt tcccgaaatt
     2161 cccgttaagg tgacgatcaa cgagtatatt gagatttcca aatcctacag ctccatcaaa
     2221 agcaaactgt ttatcaacgg gatactggat aaactggtgg ttaagctgaa agaggaaggg
     2281 aagatcaaaa aatcggggag aggactgttg gagtagtgaa gggtgaacag tgaagggtga
     2341 acagtgaagg gtgaacagtg aagggtgaa
//
LOCUS       DAOPNH010000362         2083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_68081_length_2083_cov_4.909270, whole genome shotgun sequence.
ACCESSION   DAOPNH010000362 DAOPNH010000000
VERSION     DAOPNH010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68081_length_2083_cov_4.909270"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKZ74_13980"
     CDS             complement(<1..467)
                     /locus_tag="PKZ74_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPM93908.1"
                     /translation="MNDLNDGALSPENKIIKVNIESQMKSAYIDYSMSVIVSRALPDV
                     RDGLKPVHRRVLFGMLDLGVLSNRPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQE
                     WSLRYPLIDGQGNFGSIDGDNPAAMRYTEARLRKIAEELLEDIDKNTVDFQLN"
     gene            800..>2083
                     /locus_tag="PKZ74_13985"
     CDS             800..>2083
                     /locus_tag="PKZ74_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPM93909.1"
                     /translation="MEAKFSQRVKDVLTYSREEAIRLGNNYIGLEHLLLGILREGEGL
                     AVQLMRYFGMDLNEIRKMIEKAVASPEETLHHTDNLPLVRQAERALKITYLEAKMFNS
                     ELIGTEHLLLSILRDEENIVTKFFEKNGIDYDLVREELKKMHEVKGSDDKPAPRNELP
                     GDPQDEESEEAGRMFGSTTRKQSDSKSKTPVLDNFGRDITKAAEEGRLDPIVGREKEL
                     ERIAQILSRRKKNNPILIGEPGVGKSAIAEGLADRIVKRKVSRALFDKRIISLDLASL
                     VAGTKYRGQFEERMKAVLNELEKNPDIILFIDEIHTIVGAGNASGSLDASNMFKPALA
                     RGELQCIGATTLDEYRQYIEKDGALERRFQKVLVDPTTTEETFQILNNIKEKYEDHHL
                     VKYSDDAIHACVQLTNRYITDRYLPDKAIDALDEAG"
BASE COUNT          605 a          456 c          472 g          550 t
ORIGIN      
        1 aaattcagct gaaaatcaac cgtgttttta tctatgtctt cgagaagttc ttccgcaatt
       61 tttcgcagac gtgcttctgt atacctcatg gcagcagggt tatcaccgtc gattgatccg
      121 aagtttccct gcccgtcgat caacgggtat ctcaatgacc attcctgtgc cattctcacc
      181 atggcgtcat aaaccgatga gtcgccatgc ggatggtact ttcctaatac ctccccgaca
      241 attcttgctg atttcttgta aggcctgtta gaaaggacgc ccaaatccag catcccgaat
      301 aaaaccctcc tgtgtaccgg tttcaatccg tccctgacat cgggaagagc cctggataca
      361 ataaccgaca tcgaataatc gatgtaggcc gatttcatct ggctctcgat attgacttta
      421 attattttgt tttcaggtga taatgcgcca tcatttaaat cattcataat caacatttta
      481 aattatcttc tgctatgatg cgaaggtact aatttttcat tgccgaaatc acctggggta
      541 cctattttta ttactattta ttactaacag gattctgttt atattattca taacttataa
      601 atcaacttca aagtttgaat agtagttttg tttaaggttg aaagtattgt tttacggatg
      661 aactttatag cataaccgat gtttatttta tccgtataac atttccgggg agataactga
      721 cagaggtata atgatggtcc ggtttatgtt aatgggctga ttttagctaa ttttgtcgaa
      781 tcgtttcata atttaaagaa tggaagcaaa attttcccag agggtcaaag atgttctgac
      841 atacagtcgt gaagaagcga tccgtcttgg taataattat atcggactgg aacacctgtt
      901 actgggtatt ttaagagaag gcgaaggcct tgcggtccaa ttgatgaggt atttcgggat
      961 ggatctgaat gagatcagaa aaatgattga aaaagcggtc gccagtcctg aagaaacatt
     1021 gcatcatacg gataatctgc cgctggtccg acaggctgag cgtgccctga agatcaccta
     1081 cctggaagcc aaaatgttca atagcgaatt aattggcacc gagcatcttc tgctttccat
     1141 tctgcgggat gaagaaaata tcgtcacaaa atttttcgaa aaaaatggga tcgattacga
     1201 cctggtcagg gaggagctga agaaaatgca tgaagtgaaa ggatctgacg ataaacccgc
     1261 tccccggaat gaactgccgg gtgatcctca ggatgaagag tcagaagaag ccggcaggat
     1321 gttcgggagt accacacgga aacaatccga ttcgaaatca aagacaccgg tgctggataa
     1381 ttttggccgg gatataacca aagctgccga ggaaggacgc cttgacccaa tcgttggccg
     1441 tgaaaaggaa ctggaaagga tagcccagat actaagccgg cgcaaaaaga acaatccgat
     1501 cctgatcgga gagccgggcg tgggcaaatc tgcgatagcc gaaggccttg ccgaccgcat
     1561 cgtgaaaagg aaagtatcgc gggcactgtt cgacaaacgc atcatttcac ttgatctggc
     1621 ttctctggta gccggtacca agtaccgcgg acaatttgaa gaacgaatga aagcagtgct
     1681 taatgaattg gaaaagaacc cggatataat cctgtttatc gatgagatcc atacgatcgt
     1741 tggggccggt aacgcttccg gttccctcga cgcatccaat atgttcaaac cggctctcgc
     1801 ccgcggcgaa ctgcagtgca tcggcgcaac taccctcgac gaatacaggc aatatattga
     1861 aaaagatggc gcacttgagc ggcgttttca gaaagtactg gttgatccca ctacaaccga
     1921 agagaccttc cagatcctta ataatatcaa ggagaagtat gaagaccatc atcttgtcaa
     1981 gtattctgat gatgcaatac atgcctgtgt gcaactcact aaccgctata tcacagaccg
     2041 ttacctgcct gataaagcca tcgatgccct ggacgaagcc gga
//
LOCUS       DAOPNH010000363         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_75319_length_1945_cov_5.908995, whole genome shotgun sequence.
ACCESSION   DAOPNH010000363 DAOPNH010000000
VERSION     DAOPNH010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75319_length_1945_cov_5.908995"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="PKZ74_13990"
     CDS             <1..1677
                     /locus_tag="PKZ74_13990"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HPM93910.1"
                     /translation="DLIKLPLYDPKARIQIIHNSADTMAEIVDVWLNQTLLLDNFAFR
                     TASAFMDAPANEQFTISIKGPESVDPYNPLFARNYTLVEGETYLLVADGIISESGYDP
                     DQPFDIKMYGFAREESVQTGYTDILVHHGATDAPVVDIIEVGLGAGLIVNDLAYGNFS
                     NYLELPAINYILEVRNASNSALLGTFRAPLETLGLENEAVSVVASGFVVPGNNSDGPA
                     FGLWLARAQGGALIELQAYTPLARAQVIHNSADAVVSTVDVWLDNTKILDDFAFRSAS
                     PFLDVPANTQVTIAIKGADSQDPSNPLWSKSYTFTEDEKYILVAGGILSPTGYDPQKP
                     FTVFDYEGALEQAAETTNTDVLVFHGVTDAPLVDVVVVGDGIGTIVDDLDYGLFDGYM
                     QLETGNYLLSVRDESGTTEVAYFETNLQDLNLQGKSLTLLASGFLDPTVNNDGPAFGL
                     LGVKADGSTVLFTNTLGIPEPSVNLTGLNVFPNPANDYFNVTFEMMSKGIVTVELIDI
                     MGRKAAEHYFGELNAGKVIEKITLDGIQDGYYLLRINAGDDQVVRRLIIK"
     gene            complement(1693..>1945)
                     /locus_tag="PKZ74_13995"
     CDS             complement(1693..>1945)
                     /locus_tag="PKZ74_13995"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPM93911.1"
                     /translation="IVFFSNIFAIMGLRSLFFLVSHFFSKFFYLKVGLAALLAFIGLK
                     MLTHDFYKISTDASLIVISAILGLSIIASLVHNKREQQV"
BASE COUNT          479 a          487 c          507 g          472 t
ORIGIN      
        1 gacctgataa agctccccct gtatgacccg aaagcccgca tccagattat ccacaactct
       61 gcggatacca tggcggaaat tgttgatgtc tggctgaacc agacactgct gctcgacaat
      121 tttgctttcc gcacagcatc tgccttcatg gatgccccgg ccaacgagca gtttactatt
      181 tcgatcaagg gtcctgaaag cgtggatccg tataatccct tatttgcccg taactatacc
      241 ctggtcgaag gcgagaccta tctcctggtt gccgatggaa tcatcagcga aagcggatat
      301 gatcccgatc aaccttttga tattaaaatg tatggatttg ccagggagga atctgtccag
      361 acaggatata ccgacatcct ggtccatcac ggcgccacgg atgcgccggt ggtggatatt
      421 attgaggttg gcctcggtgc cggactgatt gtgaatgatc ttgcttacgg caacttcagt
      481 aattatctcg agttaccggc cataaattat atcctggagg ttcgaaatgc atcaaactca
      541 gctcttctcg gcactttccg ggccccgctg gagacgttgg gtcttgaaaa cgaagccgtt
      601 tccgttgtgg cttccggttt tgtcgttccc ggtaacaaca gcgatggccc cgccttcggt
      661 ttgtggctgg ccagggcaca gggcggcgcc ctgatcgaat tgcaggctta cacgccgctg
      721 gcccgtgcgc aggtgattca caattcagcc gatgcagtcg tttcgacggt ggatgtctgg
      781 ctcgacaata cgaagatcct ggatgacttt gctttccgct ccgccagccc gttcctcgat
      841 gttccggcca acacccaggt aaccatcgcc atcaagggag ccgacagcca ggatccttcc
      901 aatccgctgt ggtcgaagag ctataccttc acggaagatg aaaaatatat cctggtagcc
      961 ggcggtatcc tgagcccgac cggttatgat cctcaaaagc cattcaccgt gttcgattac
     1021 gagggagcgt tggagcaggc ggcagaaacg accaataccg atgtgcttgt ctttcatgga
     1081 gtgaccgatg caccgctggt ggatgttgtt gtagttggcg acggcatcgg aacgatcgtg
     1141 gatgacctgg actatggcct gtttgatggc tacatgcaac tggaaacagg caattatttg
     1201 ctcagtgtca gggacgaatc gggaaccacc gaggtcgcct atttcgagac caacttacag
     1261 gatctgaacc tgcaaggcaa atcgttgacc ttactcgcat ccggcttcct ggatcctacg
     1321 gttaataacg acggccctgc attcgggctc ctcggcgtca aagccgatgg cagtacggta
     1381 ctgtttacga atacgctggg tatccctgag ccgtcggtca acctgaccgg cctgaatgta
     1441 tttccaaatc cggccaacga ttatttcaac gtgacttttg aaatgatgtc gaaaggaata
     1501 gttactgttg aactgatcga tatcatggga aggaaagctg ctgaacacta ctttggtgaa
     1561 ctgaatgccg gaaaagtgat cgaaaagata acgttggacg gaatacagga tggatattat
     1621 ttgttaagga ttaatgccgg ggatgaccag gtggtcagga gattgatcat taaataatcc
     1681 cgggcatcgg tcttagacct gctgttccct tttattgtgc accagcgagg caataatgct
     1741 cagtcccagt attgccgata tgacaatcag cgaagcgtct gtggagattt tgtagaaatc
     1801 atgcgtgagc attttcagcc ctatgaacgc cagcaaggca gccagcccga ccttcaggta
     1861 gaagaacttg ctgaagaaat gcgacaccag gaagaacagt gacctcagtc ccatgatcgc
     1921 aaaaatattc gagaagaaaa cgata
//
LOCUS       DAOPNH010000364         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3605
            NODE_76351_length_1927_cov_5.043269, whole genome shotgun sequence.
ACCESSION   DAOPNH010000364 DAOPNH010000000
VERSION     DAOPNH010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865576
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:07:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,773
            Genes (coding)                    :: 2,759
            CDSs (with protein)               :: 2,759
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76351_length_1927_cov_5.043269"
                     /isolate="AMR_MDS_3605"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKZ74_14000"
     CDS             <1..352
                     /locus_tag="PKZ74_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM93912.1"
                     /translation="NYELEQTSVEQEVIDFEQNVFLNVMEFNMQKDQLFIAAKADTVA
                     QKRYDVTQKRYMIGQVNDILDLNYAQIDNDNAKKGYFSALRNYWINYFQLRKMTLYDF
                     RENKLLIFDIRDIM"
     gene            458..1093
                     /locus_tag="PKZ74_14005"
     CDS             458..1093
                     /locus_tag="PKZ74_14005"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPM93913.1"
                     /translation="MSVLVGKKAPLFEADAVVNGGEFVEKFSLEQFIGKKYVVFFFYP
                     LDFTFVCPTEILAFQEKLEEFDRRNVAVVGCSVDSKYSHWAWLNTEVKEGGIKGVKFP
                     LVSDLSKTISENYDVLAGEYEYNEDGEMDFVGDAIAYRGLFLIDKKGIVRHQLVNDLP
                     LGRSIDEAIRMVDALQCYEEYGEVCPANWHAGDEAMTATHEGVSSYLAKKH"
     gene            complement(1090..1833)
                     /locus_tag="PKZ74_14010"
     CDS             complement(1090..1833)
                     /locus_tag="PKZ74_14010"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPM93914.1"
                     /translation="MKSCLIILSFLIMILAMAAGCAKDDGICAGSTGKVIRESRPVSS
                     FHYVEVWDNINLFLTQDTNATGLEIEAGENLIDGITTETERGRLIIRNRNTCNWLRSF
                     EVPVNVYLKYRWLDTLITHAAGNISGMNTWTGNTIWVTVEEGGGIIDLDLNVAKSFIT
                     LKYGTFALDFSGFSQVTFISDLGYGPLHAENLYSKFTYIYTASPNDIFVTAHVQITAE
                     IRNAGNVYYHGDPQSVSTQLIGSGRLYKF"
BASE COUNT          541 a          402 c          458 g          526 t
ORIGIN      
        1 gaactatgaa ctggaacaaa cctctgtcga acaggaggta atagatttcg agcagaacgt
       61 ctttttgaat gtgatggaat tcaatatgca gaaagaccag ctttttatcg cagccaaggc
      121 cgatacggtt gctcagaaaa gatatgatgt gacccagaaa cgttacatga tcgggcaggt
      181 aaacgatatt ctcgacctga actatgctca gattgataac gataatgcca aaaaagggta
      241 cttttccgct ctgcggaatt actggataaa ctattttcaa ctaagaaaaa tgacccttta
      301 tgattttcgt gaaaataaat tattgatttt cgatattagg gatatcatgt aacgtgttgt
      361 ttatttgcag gattgattat ttttgcccaa aatttccaaa cctcatttat tatatcaaac
      421 aatacataag aattaaaaat taaaaattat acttaaaatg tcagttttag taggcaaaaa
      481 ggcccctttg ttcgaagcgg atgcagtggt taacggcgga gagtttgtcg agaaattctc
      541 actggaacag tttatcggta agaagtatgt tgtattcttc ttttatccgc tggattttac
      601 ttttgtgtgt cccaccgaaa ttctggcttt ccaggaaaaa cttgaagagt tcgacagaag
      661 gaatgtagcc gttgtcggtt gttcggtcga tagcaagtat tcccactggg catggctgaa
      721 tactgaggtt aaagaaggag ggatcaaagg ggtgaaattc cccctggtgt ctgacctttc
      781 aaaaaccata tcagagaatt atgatgtcct tgccggagaa tatgaataca atgaggacgg
      841 cgaaatggat tttgtgggtg atgccattgc ctaccggggt ttgttcctga tcgataaaaa
      901 agggatcgtc aggcatcagc tcgtgaatga tctgccgctc ggccgcagca ttgatgaagc
      961 tatccggatg gtcgacgcac tgcagtgcta tgaggagtac ggggaagttt gcccggcaaa
     1021 ctggcacgcc ggcgatgagg ccatgacggc aacgcatgaa ggcgtttcca gctacctggc
     1081 caaaaagcat tagaacttgt aaagtcttcc gctcccaatc aattgggtgg ataccgattg
     1141 aggatccccg tgataatata cattccctgc attccttatt tcagcagtga tctggacatg
     1201 ggccgtcacg aaaatgtcat tcggactcgc tgtataaata taggtaaact tggaataaag
     1261 gttttccgca tgaagcgggc cgtaacccag atctgagatg aatgtcacct gcgagaaacc
     1321 cgagaaatcc agggcaaaag tcccgtattt taaggtaatg aaggatttgg ccacgttgag
     1381 gtccaggtcg attattcccc ctccctcttc aacggtcacc cagatggtat ttcctgtcca
     1441 tgtgttcatg cccgagatat tccctgcagc atgtgtgata agtgtatcga gccaacggta
     1501 tttgagataa acatttaccg gtacctcgaa acttctgagc cagttgcatg tattgcggtt
     1561 gcggataatg agcctgccgc gctctgtttc cgtagtgatg ccatcgatca gattctctcc
     1621 ggcttcgatc tcaaggccgg ttgcgttagt atcctgcgtc aggaaaagat tgatgttatc
     1681 ccatacttcc acataatgga atgaagatac cgggcggctt tcccggatga ccttcccggt
     1741 tgatcctgcg cagatcccgt catctttcgc gcaaccagcc gccatcgcca ggatcattat
     1801 caaaaaggat agaataatta aacagctttt catcggtaat attttaaacg aagacgatac
     1861 cctaaaccca gggtcaggta atccgctttg ccgtaatggg ctttgagggt gaaactggta
     1921 aagaggc
//
